LOCUS       DBNHXB010000001         8525 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2181093, whole genome shotgun sequence.
ACCESSION   DBNHXB010000001 DBNHXB010000000
VERSION     DBNHXB010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8525)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8525
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2181093"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..523)
                     /locus_tag="AC162G_00005"
     CDS             complement(275..523)
                     /locus_tag="AC162G_00005"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR5074227.1"
                     /translation="MQLALPGPVDRQPLAQIDAPIPVPGAGDIRLRVRACGVCHTDLH
                     IVEGDIPPARLPIVPGHQVVGAVDFGASAHIAIQVARH"
     gene            complement(529..1989)
                     /locus_tag="AC162G_00010"
     CDS             complement(529..1989)
                     /locus_tag="AC162G_00010"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5074228.1"
                     /translation="MLLSPGNVLHTRYRIVGLLGQGGMAAVYCAHDRTFDRMVAIKQL
                     RPDPFASERALAEARQQFKQEAQILATLDHPNLPRVTDYFESEGFEYLVMDYVEGQTL
                     SDLVAQHGNGLDEGQVLDWADQLLAALEYIHQHEHVHRDVKPSNIRLTPDGRIFLVDF
                     GLVKLFDPTNPKTATIMHGLGTPEYAPPEQYDAHVGHTDPRSDVYALGATMYHLFTGR
                     APATATQRVADPSSFRAPHALGAHISADVERAILRALELQPAKRFDSATTMRAALRQA
                     RRQVTVADATMTRRLPRWSAPLSRTNWRRFAAIGAIALLLAGGVIVQSGSAVPGGLPP
                     ALGTTATPTGSIASPSSVVSSPTAPAVVASPTRTLRPSPTRSNTPTLTATSTPTSTLA
                     ATPTGTRTRTRTATPTRTSTPTPTFTSRPSQPTNTPTPTPEPTTPIPTTPVPTTPPPT
                     TPPRPIPTVPTDTPVPTDTPAATVEPAASTTPATPP"
     gene            complement(2067..2636)
                     /locus_tag="AC162G_00015"
     CDS             complement(2067..2636)
                     /locus_tag="AC162G_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074229.1"
                     /translation="MEQNFDERAGDVRGDTVNIQDGGARDVYATNVTLSRGGARSVKA
                     DTVTVRQGGVQKVEAQSIALKQGGALSVAAERAEIVASAAAFVRAGDARIGPSCRVGG
                     LIAQQTVVEDSMLAAVLARGEARLEQSAVGVVVSNRAVLSGSAVGFAVAEVVEGDVRV
                     QVKPQVAALFGAAFGAALGLLVLIGRRIR"
     gene            complement(2731..3558)
                     /locus_tag="AC162G_00020"
     CDS             complement(2731..3558)
                     /locus_tag="AC162G_00020"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HIR5074230.1"
                     /translation="MSDYATDYTRDELMCVCISRQVRDGEAVGQGIATPLVMAGVILA
                     KLTHAPSVLLASAIGQSVCRDWAPLGLARVEEHWIGKGLTFLTFSEVSCELLPRVRVK
                     EFFRPAQVDSRGNFNNVVVGDRYERPQMRLPGSGGIPDVTGYNDKSCLYVPRHSRAVF
                     VEPIDFVSGLGHVSRRADGVGPVYLVSNLGQFDFASGRMRLTTFHACESVERIQAKTG
                     FPLEIAPDVHETEPPSAEEVRLLRDVIDPLGVRRLELLSGAQRKQLIRDILRKEGVL"
     gene            complement(3634..4485)
                     /locus_tag="AC162G_00025"
     CDS             complement(3634..4485)
                     /locus_tag="AC162G_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR5074231.1"
                     /translation="MLDFLLDPLAHSFMQRALLASVLAGGLCAIVGCYVVLRGMAFLG
                     DALAHAVLPGIAIAFLLGGDLFAAALATAILVALGMGYLGRSGRLREDAAIGVVFSGA
                     FALGVALISSAPSYQTDLTHILFGNVLGVTDRDLLLLIGFGMAVLVIVLALYKELLAF
                     TFDPTFASSIKLPVEKLRYLLMVVLAVTVVVSLRTVGVGLVAALLVTPAAAASMLTRR
                     LSRMMVVSALIGVASAVVGLYVSYYGDLSAGASIVLTCTVLFLLALVFSPRDGMLARL
                     RPERRSG"
     gene            complement(4587..5384)
                     /gene="aztA"
                     /locus_tag="AC162G_00030"
     CDS             complement(4587..5384)
                     /gene="aztA"
                     /locus_tag="AC162G_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein AztA"
                     /protein_id="HIR5074232.1"
                     /translation="MAAGKHAHSRHIAIHLDGVSVAYDGKLALTDVTLDISGGLRLAV
                     LGPNGAGKSTLFKAIVGLLPLRSGHILIHGDPPRQREKHIAYVPQHGDVDRRFPVTVR
                     DVVMMGRYGRIGWLRRPGRDDRRAVDQALQRLDIARLASAPIDELSGGQLQRAFLARA
                     LAQDADVLLLDEPFTGVDAATESAIYQVLDDLKARRMTVLVATHDLDKAMSHFDDLLL
                     VNGTVIACGCAEDVFRPEILRRAFGSQVTLMPTGERVMAATDEHYSQ"
     gene            complement(5400..6350)
                     /locus_tag="AC162G_00035"
     CDS             complement(5400..6350)
                     /locus_tag="AC162G_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013465.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter solute-binding protein,
                     Zn/Mn family"
                     /protein_id="HIR5074233.1"
                     /translation="MKRLLSMGCIVALAAGACAGATGSPDSLPDGRLPVVATHSILGE
                     FVTIVGGDRVAVRTLVGPQGDAHEFEPSPADAAAIAEAAILFENGLGFEPWLDDLYAA
                     SDSGAVRVVATDGIVPLPAASLSGRGGAEDGPGDLGQLDPHVWHDVTNAMRMVENVQA
                     ALLKTDPPHADVYAANADAYLAQLTELDAWVRQQVERLPAGRRMLVTSHDTLGYFAAR
                     YGFDVVGTALGGTTAGLADPSAGAIAELVEAIKAAGVPAIFAENVSNPGLMQQIAAEA
                     GVELAPLLYTDALGEPGSAGDTYVKMIRYNVSTIVGALSK"
     gene            complement(6347..6802)
                     /locus_tag="AC162G_00040"
     CDS             complement(6347..6802)
                     /locus_tag="AC162G_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR5074234.1"
                     /translation="MFDRDFVTRQLAARQFKITRQRRAVLNAIASSQARMSAADICAR
                     ARRECRDLGLATVYRTLEILAEIGAIRRVHLDDDCEGFAAAAAEHGHHLVCSRCGAAL
                     EIEGCDLTGMLERVEASTGFKVDQHWLELVGRCPACQARPRQRALGRQP"
     gene            7312..8424
                     /locus_tag="AC162G_00045"
     CDS             7312..8424
                     /locus_tag="AC162G_00045"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018396259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5074235.1"
                     /translation="MQEHTIELRDVVKQFPGATRGGDPVLAVDHVTLQIRDGEFFSLL
                     GPSGCGKTTTLRMIAGFETPTAGEIYIHGRSIGHTPPYQRPVNTVFQHYALFPHMTVG
                     QNVAFGLEMKKAPRGEIKKRVDEALELVRLPGMHDRMPKQLSGGQQQRVALARALVNH
                     PEVLLLDEPLGALDLKLRKEMQLELKQLQERVGITFIYVTHDQEEALTMSDRIAVMDH
                     GRALQIGTSLEIYEAPNSRFVADFIGETNFISGKVTSLNGADAEFEADGLKMIAAVEA
                     GVGLGAQATLAVRPERIVLSDTQPEDTPNAYLGEVIEMIYVGNDTRFRVQLAPRITLS
                     VRRQNILPIGLPALQGPTGMRVWVSWHPGSARVLVN"
BASE COUNT         1555 a         2862 c         2755 g         1353 t
ORIGIN      
        1 gtcgccgacg acgacgttgt tgaaattgcc tcgcgagtcg acctacgccg ggcggaaaaa
       61 ctctttcaca ctgacgcgcg gcagcagctc gcaggacact tcgctgaagg tcaaacatgt
      121 cacattatac gcccaaatgt gcgattgggg ttgcggtcaa ccggagcggc gctccggccg
      181 caaacgggcg agcatgccgt cgcgcgggct gaagaccagc gccagcagga acaatacggt
      241 gcaggtcagc acgatggatg cgcctgctgc aagatcaatg ccgcgccacc tgaatggcaa
      301 tgtgcgccga cgcgccgaag tcgaccgcgc ccacgacctg gtgccctggc acgatcggca
      361 gcctggcggg cgggatgtcg ccttcgacga tgtggagatc ggtatgacac acaccgcagg
      421 cgcgaacgcg gaggcggatg tcgcccgcgc cgggaacggg gatcggcgcg tcgatctgcg
      481 ccaggggctg cctgtcgacc ggcccaggca gcgccaattg catggcaatc acggcggcgt
      541 agcgggcgta gtgctcgcag cgggttccac cgtcgcggcc ggcgtgtctg tcggaacggg
      601 cgtgtctgtc ggaacggtgg gaattggtct gggtggcgta gtaggtgggg gtgtcgttgg
      661 cacaggcgta gttggaatcg gagtcgtcgg ctcgggcgtc ggggtaggtg tattcgtggg
      721 ctgtgaaggc cgactggtga acgtcggggt tggcgtcgac gtgcgcgtcg gcgtcgccgt
      781 tctcgtccgc gtccgcgtgc ccgtcggcgt ggccgccagg gtcgacgtcg gggtcgacgt
      841 cgccgtcaag gtcggtgtgt tggaccgcgt cggcgacggc ctcagcgtac gcgtcggtga
      901 tgcaaccacc gccggcgcgg tcggggacga gaccacagac gacggagatg cgatagaacc
      961 tgtcggggtg gcggtcgttc ccagagcagg cggcaacccg cccggtacgg cgctgccaga
     1021 ctgtacgatc actccaccgg cgaggagcag cgctatcgcc ccaatggcgg cgaagcggcg
     1081 ccaattcgtg cgcgacagcg gtgcgctcca gcgcggcaac cggcgcgtca tggtcgcgtc
     1141 cgccacggtc acctgccgcc gcgcctgcct gagcgccgcg cgcatcgtgg tcgcgctgtc
     1201 gaagcgcttg gccggctgca gctccaacgc gcgcaggatg gcgcgctcca cgtccgccga
     1261 gatgtgcgcc cccagcgcgt gcggcgcccg gaacgacgac gggtcggcga cccgctgcgt
     1321 cgccgtggcc ggcgcgcggc ctgtgaacag atggtacatc gtcgcgccca gcgcgtacac
     1381 atcggagcga ggatcggtat ggcccacgtg cgcgtcgtac tgctccggcg gcgcatactc
     1441 gggtgtgccc aggccatgca tgatcgtcgc cgtcttgggg ttcgtcggat cgaacagctt
     1501 gaccagcccg aagtccacca ggaagatgcg cccatcgggg gtgaggcgaa tgttcgacgg
     1561 cttgacgtcg cgatgaacat gctcgtgctg gtgaatgtac tcgagcgccg ccaggagttg
     1621 atccgcccag tccagcacct gcccttcgtc caggccgttg ccgtgctgcg ccaccagatc
     1681 gctgagcgtc tggccttcga cgtagtccat caccagatac tcgaagccct cgctctcgaa
     1741 gtaatcggtc acgcgcggca ggttgggatg gtcgagcgtg gccaggattt gcgcttcttg
     1801 cttgaactgc tgccgcgcct cggcgagggc tctttcgctc gcgaaggggt cgggacggag
     1861 ctgcttgatc gccaccatcc ggtcgaaggt gcgatcgtgc gcgcagtaca ccgccgccat
     1921 gcccccttgc cccagcaggc cgacgatgcg gtagcgcgtg tggagaacgt tacccggcga
     1981 cagcagcatt gtgcatcctc aatcttcgcg cgcccgagcc gcctgcgggc gctggcccga
     2041 taccggagct gtgcggagtc ggcctctcag cggatgcgcc gaccgatcaa gaccaacagc
     2101 ccgagcgctg cgccgaacgc cgcgccgaac agcgccgcga cttgcggttt gacctgcacg
     2161 cgcacatcgc cttccacgac ttcggctacg gcgaagccga cggccgaacc ggagagcacc
     2221 gcccgattcg atacgacgac gcccaccgcc gactgctcga gacgggcctc gccccgcgcc
     2281 agcaccgcgg ccagcatgct atcttctacg accgtctgct gggcgatcag cccacccacg
     2341 cgacagctcg ggccgatgcg ggcgtccccg gcgcgcacga aggccgcggc cgaggccacg
     2401 atctcggctc gctctgcggc gacgctcaag gccccaccct gcttcagcgc gatcgactgc
     2461 gcctcaactt tctgaacgcc gccttgtctc accgtcaccg tgtcggcttt cacactcctc
     2521 gcgccgccgc gcgaaagcgt gacgttcgtc gcatacacat cgcgcgcgcc gccatcctga
     2581 atgttgaccg tatcgccgcg cacgtcgccg gcccgctcgt cgaaattctg ctccatgtcg
     2641 cgcacctctt cccgtctccg acctgcctcc tagtatactc catatgtgca agaggcgatc
     2701 aggtgaatgc ccaggcggca aagcgccgga ttaaaggacg ccctctttcc gcaggatgtc
     2761 ccgaatgagc tgcttgcgct gcgccccgct cagcagctcc agccggcgca cacccagcgg
     2821 atcgatcacg tcgcgcagca ggcgcacttc ctccgccgag ggtggctcgg tctcgtgaac
     2881 gtccggcgca atctcgagcg ggaaacctgt cttcgcctgg atcctttcca cgctctcgca
     2941 ggcgtggaaa gtcgtcagcc gcatacggcc gctggcgaag tcgaactggc caaggttgct
     3001 gaccaggtac accgggccaa cgccatcggc gcgacgagac acgtgcccca gcccactgac
     3061 gaagtcgatc ggctcgacga acacggcgcg ggagtggcgc ggcacgtaca ggcacgactt
     3121 gtcgttgtag cccgtgacgt ccgggatgcc gccgctgccc ggcagccgca tctgcgggcg
     3181 ctcgtaacgg tcgccgacga cgacattgtt gaaattgcct cgcgagtcga cctgcgccgg
     3241 gcggaaaaac tctttcaccc tgacgcgcgg cagcagctcg caggacactt cgctgaaggt
     3301 caagaacgtc agccctttgc cgatccaatg ctcctcgacg cgcgccaggc ccagcggcgc
     3361 ccaatcgcgg cacacagact ggccgatggc cgaggccagc aagacgctcg gcgcatgcgt
     3421 caacttggcg aggatcacgc ccgccatcac caacggcgtg gcgatgccct gcccaaccgc
     3481 ctcgccgtcg cggacctggc gcgagataca cacacacatc aactcgtcgc gagtgtaatc
     3541 cgtcgcgtaa tcagacatat tgggcagcgc actcgccgat gaggcgcaca gacatgtcac
     3601 attatacgcc caaatgtgcg attggggttg cggtcaaccg gagcggcgct ccggccgcaa
     3661 acgggcgagc atgccgtcgc gcgggctgaa gactagcgcc aggaggaaca atacggtgca
     3721 ggtcagcacg atggatgcgc ctgccgaaag gtcgccgtag taggacacgt ataacccgac
     3781 gactgccgac gccacgccta tgagagcgga gacgaccatc atgcgtgata aacgccgggt
     3841 caacatcgag gccgccgcgg ccggcgtgac gagcagggct gcgaccaggc cgacgccgac
     3901 cgtgcggagc gagacgacga cggtgacggc caacacgacc atgaggagat agcgcagctt
     3961 ctcgaccggc agcttgatcg acgacgcaaa ggtcggatca aacgtgaagg cgagcagctc
     4021 cttgtacagc gccaggacaa ttaccaagac cgccatcccg aagccgatca gcaacaggag
     4081 atcgcgatcg gttacgccca gcacgttgcc gaagaggatg tgcgtcagat cggtctggta
     4141 ggagggcgcg ctgctgatca gggcgacgcc gagcgcgaag gcgcccgaga agacgacgcc
     4201 gatcgccgca tcctcgcgca gccgcccgct gcggcccagg tatcccatgc cgagggcgac
     4261 gagaatcgcc gtcgccaacg ccgcggcaaa caagtcgccg cccagcaaga aggcgatcgc
     4321 gatgccgggc aagacggcgt gcgcgagcgc gtcgccgagg aaggccatgc cgcgcagcac
     4381 gacgtagcac ccgacgatag cacacaaccc gccggccagc acggacgcga gcagcgcgcg
     4441 ctgcatgaag gagtgagcga gaggatccag caagaagtcg agcatgcgca agctaacaga
     4501 agatggtagt cacgacttca atcgttacag cagcgactaa agtcgccact acgtttcgtt
     4561 tggcgtgatg cttttcagac aggctcttac tgcgaataat gctcgtctgt ggcggccatg
     4621 acgcgctcgc ccgtcggcat gagcgtcacc tgagacccga atgcccgccg cagaatctcc
     4681 ggccggaaca catcctcggc gcagccgcag gcaatcaccg tcccgttcac cagcaagaga
     4741 tcgtcgaagt gagacatggc tttgtccagg tcgtgcgtag cgaccagcac cgtcatgcgg
     4801 cgcgccttta gatcgtccaa cacctggtag attgcgctct cggtcgctgc gtcgacgccg
     4861 gtgaacggct cgtccagcag cagaacgtcg gcatcctggg ccagcgcgcg agccaggaag
     4921 gcgcgctgca actgtcctcc ggaaagctcg tcgatgggtg cgctcgccag gcgcgcgatg
     4981 tcgagccgct gcagcgcctg atcgaccgcg cggcgatcgt cgcggcccgg ccggcgcagc
     5041 cacccaatcc gcccatagcg ccccatcatc accacgtcgc gcacggtcac cggaaaacgc
     5101 cgatcgacgt cgccgtgctg gggcacgtag gcaatgtgct tctcgcgctg gcgaggcggg
     5161 tcgccgtgga tcaggatgtg tccggagcgc agcggcagta acccgacgat ggccttgaac
     5221 agggtcgact tgcccgcgcc gttcggcccg agcacggcca ggcgcagccc cccggagata
     5281 tccagcgtca cgtcagtcag ggcgagcttg ccgtcgtaag ccacactcac gccatcgaga
     5341 tgaatggcga tatgcctgga atgggcgtgt ttgccggctg ccatcgcatc cctacgcggt
     5401 catttgctca gtgcgccgac gatggtcgac acgttatacc ggatcatttt gacgtacgta
     5461 tcgccggcgc tgcccggctc gccgagcgcg tcggtataca gcaggggagc cagctccacg
     5521 ccggcctcgg ctgcgatttg ttgcatcagg ccaggattcg acacgttctc ggcgaagatg
     5581 gcggggacgc cggctgcctt gatggcctcc accagttctg cgatcgcgcc ggccgacgga
     5641 tcggcaagcc cggccgtcgt tccacccagc gccgtgccga ccacgtcgaa gccgtagcgc
     5701 gcggcaaaat agccgagcgt gtcgtgcgac gtgacgagca tgcgacgtcc ggcgggcaac
     5761 cgctcgacct gctgcctcac ccaggcgtcc aactccgtca actgcgcaag gtaggcatcc
     5821 gcgtttgcag cgtacacgtc cgcatgcggc ggatcggttt tcagcagcgc agcctgcacg
     5881 ttttcgacca tgcgcattgc attcgtcaca tcgtgccaga cgtgaggatc gagctggccc
     5941 aaatcgcccg ggccatcctc agcccctccc ctcccagata gactcgctgc cggaagaggc
     6001 acaatgccat ccgtcgcgac aacccgcacc gccccagagt cagacgcggc atacagatcg
     6061 tccagccacg gctcgaatcc caggccgttc tcgaagagga tagccgcttc ggcaatggcg
     6121 gccgcatccg ccggactagg ctcgaattca tgcgcatctc cttgcgggcc gaccagggtg
     6181 cgcacggcga ccctgtcgcc gcccacaatc gtcacaaact cgcctaagat gctgtgcgta
     6241 gccacaaccg gcagccggcc atcgggcagg ctatccggtg agcctgtcgc gccggcgcat
     6301 gcgcctgccg cgagcgcgac gatacagccc atcgagagca gcctcttcac ggttgcctcc
     6361 cgagcgcccg ctgccgcggc ctggcctggc atgccgggca gcggcccacc agctcgagcc
     6421 agtgctgatc caccttgaag ccggtgctgg cctcgacgcg ctcgagcatc cccgtcagat
     6481 cgcagccttc gatttcgagc gcagcgccgc accggctgca aacgaggtga tgcccgtgct
     6541 cggcggcggc ggccgcgaac ccttcacagt cgtcgtcgag gtgcacgcgc cgtatcgcgc
     6601 cgatctcggc aagaatctcc agcgtgcggt acacagtcgc caggcccaag tcgcggcact
     6661 cgcgcctggc cctggcgcaa atatcggccg cgctcatgcg cgcctgcgac gacgcgatcg
     6721 cgttcagcac cgcgcggcgc tggcgcgtga ttttgaactg ccgcgcggcg agctgccgtg
     6781 tgacgaagtc ccggtcaaac atgcaaacgt ccaattgaga agcgttctca attagagaga
     6841 ttctagcacg cgtgcctgtt gacgtcaagg cgcaatcacc cggtgattgc ccaccagtgc
     6901 tcaagccgcc gtctcggcgg cgcatcgctc gcgccggggc ctgccctgcg aagtccccga
     6961 ccccgagcaa agcgaggggg agtggggagc ctgacgaagg gacggcgctg ggagcgaaat
     7021 gtgggtaatc accaggcgat cccggtggac gactccgatc gcgccgcgga gataccagag
     7081 cgcggagcag acttatttgc cgtatgtcgc tttgatgcta taatcattct cgcctcaagc
     7141 cgcgctcctc cggtcaggga gtgagcagtg tcaattttca gtccaagggt tacaacccgc
     7201 cgtggcgcat agcaggtgcc catcatttgt tgtgtgcacg ttgccgtcag gtcgtgattg
     7261 acggttggcg attcggcagc cgcacgcgcc ggccgccggt gaggagatcg aatgcaagag
     7321 cacaccattg agctgcgcga tgtggtcaag cagttccccg gagcaacgcg cgggggcgat
     7381 ccggtgctgg ccgtcgatca cgtcacgctc cagatccgcg acggcgaatt cttctcgctc
     7441 ttgggaccca gcggctgcgg aaaaacgacg acgctacgca tgatcgccgg gttcgagacg
     7501 cccactgcgg gcgagatcta catccacggc cggtccatcg gtcatacccc gccgtaccag
     7561 cgcccggtca acaccgtgtt tcagcattac gccctgtttc cccacatgac ggtgggccaa
     7621 aatgtcgcct tcgggcttga gatgaaaaaa gcgccccgcg gcgagatcaa gaagcgcgtc
     7681 gacgaggcgc tcgagctggt gcgcttgccg ggtatgcacg atcgcatgcc caagcagcta
     7741 tccggcggcc agcagcaacg cgtcgccctg gcccgcgcac tggtcaacca tccggaggtg
     7801 ctccttctgg acgagccgct cggcgcgctt gacttgaagc tgcgcaagga aatgcagctc
     7861 gagctcaagc agctccagga gagagtcggc atcacgttca tctacgtgac ccacgaccag
     7921 gaagaagccc tgaccatgtc cgatcgcatt gcggtcatgg atcacggcag ggcgctgcaa
     7981 atcggcacgt cactagagat ttacgaggcg ccgaatagtc gtttcgtcgc cgacttcatc
     8041 ggcgagacca acttcatcag cggcaaggtc acgtcgctca acggcgcgga cgccgagttc
     8101 gaggccgacg gcctgaagat gatcgccgcg gtcgaggccg gcgtggggtt gggcgcgcag
     8161 gcgacgcttg ccgtgcgacc cgagcgcatc gtgctgtccg acacgcagcc cgaagatacg
     8221 ccgaacgcct atctaggcga agtgatcgag atgatctacg tcggcaacga tacgcgcttc
     8281 cgggtgcaac tggcgccgag gatcacgctc agcgtgcgcc ggcaaaacat tctcccgatc
     8341 ggcctgccgg cgctgcaagg ccctaccggc atgcgcgtct gggtgtcctg gcacccgggc
     8401 agcgcgcgcg tgctggtcaa ctgacggcga ggcggcggcc atgctcagac gactgcgcga
     8461 gaacaagtcg ctccggggca ccctgctggc ctcacccgcc atgttgtgga tgatcatcct
     8521 gctga
//
LOCUS       DBNHXB010000002         4887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_168278, whole genome shotgun sequence.
ACCESSION   DBNHXB010000002 DBNHXB010000000
VERSION     DBNHXB010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_168278"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1616
                     /locus_tag="AC162G_00050"
     CDS             <1..1616
                     /locus_tag="AC162G_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HIR5074236.1"
                     /translation="PSEQFKGTALFDEIIEGDLLEGALAFAEKVVAQKLPLKRVRDIE
                     IDTSNAEGFLQFARNNIAVAAKNLPAPLKCVDAVAAAVSKPFDEGLQVERELFAYLME
                     TPESKALRHAFFAERAASKIPDVPEDTPTRPIRKVAIIGAGTMGGGISMNFLNAGIPV
                     TLLEIKQEALDRGVSTIRKNYENSMKKGRLTPDQLEERMGLLQPTLSYDDIQDADLVI
                     EAVFEDMGVKESVFKTLDELAKAGAILATNTSTLDVNKIAAFTKRPQDVVGTHFFSPA
                     NVMKLLEVVRGEKTAKDVLATVMQLAKTIKKTAVVSGVCDGFIGNRMLEPYSRQALFL
                     LEEGASPQQVDQALEKFGFAMGVFRVGDLAGNDIGWAIRKRRYVEKPHVHYSKIADRL
                     CEQGRFGQKTGMGWYRYEAGRRDAIPDPAVEELIDNYRKEIGITSRPISDEEIVARCV
                     FALVNEGARLLEEGIAQRASDIDMVYLTGYGFPLHRGGPMFYADTVGLYSVVRAMGRF
                     AQNSHADPKSWEPAPLLAKLAEEGKTFNG"
     gene            1695..2873
                     /locus_tag="AC162G_00055"
     CDS             1695..2873
                     /locus_tag="AC162G_00055"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR5074237.1"
                     /translation="MNEAVVVSTARTGLAKSWRGSLNMTHGATIGGHVVKHAIERAEL
                     DPGEVEDVLMGCALPEGTTGGNIARQIALRAGCPVTVPGATVNRFCSSGLQTIVMAAQ
                     RIIAGEGDIFVAGGVESISCVQNEANRHMAREPWLLEKKPEIFWSMLQTAENVSQRYN
                     IPREKQDEYGVQSQQRAAAAAAAGKFDDEIVPITVTMGVADKASGRLYTKEVTLAADE
                     GIRPDTTYEGVSKIRPALPGGVIAAGNASQFSDGASAAVVMNAKVAEKKGLQPLGIFR
                     GFAVAGCEPDEMGIGPVFAVPKLLERAGKRIEDIDLWEMNEAFAVQVIYCRDRLGIPN
                     DMLNVDGGAIAVGHPYGVSGARLVGHALIEGRRRGAKYVVTTMCVGGGMGAAGLFEVA
                     "
     gene            2898..3671
                     /locus_tag="AC162G_00060"
     CDS             2898..3671
                     /locus_tag="AC162G_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5074238.1"
                     /translation="MGVKELFDIGGKAALITGGSRGLGLQMAEALGEMGARVAITARK
                     SAELEQAAAHLEKLGIDALTIVNDLSKFDTIPGLVDQVVSGLGDIDILVNNAGATWGA
                     PAEDYPAEGWHKVINLNVNGLFFLTQEVGKRCMIPKRAGKIVNIASVSGLSGNSLGVR
                     TIAYNTSKGAVVNFTRALAAEWGKYNINVNAICPGFFPTKMSRGVLEQITPAVIAATP
                     LQRIGGDEDLKGVIAFLASEASRHITGQTIVVDGGASIA"
     gene            3681..4109
                     /locus_tag="AC162G_00065"
     CDS             3681..4109
                     /locus_tag="AC162G_00065"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR5074239.1"
                     /translation="MEKSLFGLSIPFLELLGVQAEHREKGRAVVSLDVRHELTNSWEV
                     AHGAVVVALLDVSMAAAARSVNDHAEGVVTVEMSVNFMKSGAGRLTAEGRVLRNGRAL
                     VFCEGEVRDDSGRLVAKAIGTFKLRRRRHGTSSRSKRKNT"
     gene            4106..>4887
                     /locus_tag="AC162G_00070"
     CDS             4106..>4887
                     /locus_tag="AC162G_00070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR5074240.1"
                     /translation="MSEMNQQILLAHRPTGAPQTSDFRWVETAVPEIGDGQFLVRSHY
                     LSLDPYMRGRMSEAKSYAKPVELGEVMIGGAVGQVVTSRHPKFKAGDMVVGNFGWQLY
                     AVSDGAGVRKITDRNLPLSAYLGVVGMPGVTAYVGLLDIGQPKSGETVVVSAAGGAVG
                     GVVGQIARIHGCRTVGIAGGPEKCRYVVEELGFDACVDYKTGRLWEDVKEATPQGIDV
                     YFDNVGGEILDTILRRMNPFGRIPLCGVISQYNATQPYGVKNT"
BASE COUNT          965 a         1409 c         1634 g          879 t
ORIGIN      
        1 ttccctccga gcaattcaaa gggaccgcgc tgttcgacga gatcatcgag ggcgatctgc
       61 tcgagggcgc gctcgccttc gccgagaagg tcgtggcgca gaagctgccg ctcaaacgcg
      121 tgcgtgacat cgagatcgac acctcaaacg cagaaggctt cctccagttc gcccgaaaca
      181 acatcgccgt ggcggcgaag aatctccccg cgccgctcaa atgtgtggac gccgttgcgg
      241 ctgccgtgtc caaacccttc gatgagggct tacaggtcga gcgcgagcta ttcgcgtatc
      301 tgatggaaac gcccgagtct aaggcgctgc gtcacgcctt cttcgccgag cgtgcggcct
      361 ccaagattcc ggatgtaccg gaggacacgc cgacgcgccc gatcaggaaa gtcgcgatca
      421 tcggcgccgg cacgatgggc ggcggcatca gcatgaactt cctcaacgcc ggtatccccg
      481 tgacactcct cgagataaaa caagaggcac ttgatagggg cgtctcaacc attcgcaaga
      541 actacgagaa ttcgatgaag aagggcagac tgacgcccga tcagttggag gagcggatgg
      601 ggttgctcca gccgacgctg tcttacgacg acatccagga cgccgacctg gtgatcgagg
      661 cggtattcga agacatgggc gtcaaggaga gcgtgttcaa gacgctcgac gagctggcaa
      721 aagccggtgc tatcctggcc accaacacct ccacgctcga cgtgaacaag atcgccgctt
      781 tcaccaaacg gccccaagat gtagtcggca cccatttctt cagcccggcc aacgtcatga
      841 agctgctgga agtggtgcgc ggtgagaaaa ccgccaagga tgtgctggcg accgtgatgc
      901 agttagccaa gacgatcaag aaaaccgcag tcgtgtcggg cgtgtgcgat ggtttcattg
      961 gcaatcgcat gctcgagcct tactcgcggc aggccttgtt cctgctcgaa gaaggggcct
     1021 cgccgcagca ggtggatcag gcccttgaga agttcggctt cgctatgggg gtgtttcggg
     1081 tcggcgacct ggccggcaac gacatcggct gggccatccg caagcgccgc tacgtcgaga
     1141 agccgcacgt gcactattcc aagatcgcgg atcgtctgtg cgagcagggc cgctttgggc
     1201 agaaaaccgg catgggctgg taccgctacg aggccggccg gcgcgacgcc attcccgatc
     1261 cggcggtgga agaattgatc gataattacc gcaaagagat cggcatcacc tcgcgcccga
     1321 tcagcgacga ggaaatcgtc gcgcgctgcg tcttcgccct ggtgaacgaa ggcgcgcgcc
     1381 tcctcgaaga gggcattgcg cagcgtgcct ccgacatcga catggtttac ctgaccggtt
     1441 atggtttccc gctgcaccgg ggggggccta tgttctatgc cgatacggtc ggcctgtaca
     1501 gcgtcgtgcg cgccatgggc cgctttgctc aaaactcgca cgccgacccg aagtcctggg
     1561 agcctgcgcc tctgctggcc aagctggccg aggaaggcaa aacgttcaac ggttgaccct
     1621 cggagcgcac aagacgagcg ccttgtctgc gctccgaggg ttgatgggtc ttctcattct
     1681 ccaggagtga catcatgaac gaagcagtag tcgtttctac cgcacgcacc ggcctggcca
     1741 aatcctggcg cggatcgctc aacatgaccc acggcgcgac gatcggcgga catgtcgtca
     1801 agcatgccat cgaacgcgcc gaactcgacc cgggggaggt cgaggatgtg ttgatgggct
     1861 gtgccctccc tgaaggcacc actggcggca atatcgcccg ccagatcgcg ctgcgcgccg
     1921 ggtgcccggt cacggtgccg ggtgcgacgg tcaaccgctt ctgctcgtcc ggcctgcaga
     1981 cgatcgtcat ggccgcccag cgcatcatag ccggcgaagg ggacatcttc gtggccggcg
     2041 gtgtggagtc catctcctgc gtgcagaacg aggcgaacag acatatggcg cgcgaaccct
     2101 ggctgctgga gaaaaagcca gagatcttct ggtcgatgtt gcagacggcg gagaacgtgt
     2161 cccagcgcta caacattccg cgcgaaaaac aggacgagta cggcgtgcag agccagcaac
     2221 gcgcggcggc agccgcggcc gcgggcaagt tcgatgatga aatcgtgccg atcacggtga
     2281 cgatgggtgt ggcggataag gcgtccggtc gcctgtatac aaaagaggtg acactcgcgg
     2341 cagacgaggg tatccgtccc gacaccacct acgaaggtgt gtccaagatc aggccggcgc
     2401 tgccgggcgg cgtgatcgcc gccggcaacg ccagccagtt ctccgatggc gcttcggctg
     2461 cggtggtgat gaatgccaaa gttgccgaga agaaagggtt gcagccgctg ggcatcttcc
     2521 gtggcttcgc cgtggccggt tgtgagcctg atgagatggg cattggtccc gtcttcgcgg
     2581 ttccgaagct gctcgagcgg gcgggcaaga ggatagagga tatcgatttg tgggagatga
     2641 acgaagcctt tgccgtgcag gtgatctatt gccgcgaccg cctcggcatt ccaaacgaca
     2701 tgctcaacgt cgacggcggc gcgatcgccg tggggcatcc ctacggtgta tccggcgcgc
     2761 gtctggtggg ccacgcactg atcgagggca ggcgccgtgg cgcgaagtac gtcgtaacca
     2821 ccatgtgcgt cggcggcggc atgggagccg ccggcctgtt cgaagtggcg taaggggaac
     2881 gcagaaagga atccagaatg ggcgtgaagg agctatttga tatcggcggc aaagcggctc
     2941 tcattaccgg cggttcgcgt gggctgggac tgcagatggc cgaagccctg ggcgaaatgg
     3001 gcgccagggt ggcgatcacc gcgcgcaaat cggccgagct ggagcaggcg gccgcccatc
     3061 tggagaagct gggcatcgat gccctgacta tcgtcaacga cctgtccaag ttcgatacta
     3121 ttcccggtct ggtcgatcag gttgtgagcg gactcggcga catcgatatt ctggtcaaca
     3181 acgccggggc gacatggggc gcgccggccg aggattatcc ggccgagggc tggcacaagg
     3241 tgatcaacct caacgtcaat ggcctgttct tcttgacaca ggaggtggga aagcgctgca
     3301 tgattccgaa gcgcgcgggt aagatcgtga acatcgcttc ggtctccggg ttgagcggca
     3361 attcgctagg tgtgaggacc atcgcctaca acaccagtaa gggtgccgtg gtcaatttca
     3421 cgcgcgcgtt ggcggcggag tgggggaagt acaatatcaa cgtgaacgcc atctgtcctg
     3481 ggtttttccc caccaagatg tcgcggggag tactggagca gataacgccg gccgtcatcg
     3541 ccgcgacacc gctccagcgg atcggaggtg acgaggattt gaaaggtgtg atcgcgttcc
     3601 tggcttccga agcctcgcgc cacatcaccg gccagaccat cgtcgtggac ggcggcgcat
     3661 cgatcgcgtg atggtgcctt gtggaaaaat cgttgtttgg cctgtctatc cccttccttg
     3721 agctattggg cgtgcaagcc gagcatcggg aaaaggggcg ggctgtggtg tcgctcgatg
     3781 ttcggcacga gcttacgaac agttgggaag tggcccacgg cgccgtggtc gtggcgctgc
     3841 tcgacgtgtc gatggccgcg gcggcacgca gcgtcaacga tcatgccgag ggagtagtga
     3901 cggtggagat gtcggttaac ttcatgaagt ctggcgcggg ccggctgaca gccgagggcc
     3961 gggtgctgcg taacggccgc gcactggtgt tttgcgaagg cgaggtacgc gacgacagcg
     4021 gccggctcgt ggccaaggcc atcggcacct tcaagctgcg tcgccgccgg catggaacat
     4081 cgtcccgatc caagaggaaa aacacatgag cgagatgaat cagcagatcc ttctggctca
     4141 tcgtccgacc ggcgcgccgc agacaagtga cttccggtgg gttgagacgg ccgtgccgga
     4201 aatcggcgac gggcagttcc tggtgcgcag tcactatctc tcactcgatc cctacatgcg
     4261 cggtcgcatg agcgaggcga agtcgtacgc caagccggtt gagttgggcg aggtgatgat
     4321 cggcggcgcc gtgggccaag tggtgacctc gcgccatccg aagttcaaag cgggcgacat
     4381 ggtcgtgggt aacttcggct ggcagctcta tgctgtgagc gacggcgcgg gcgtacgtaa
     4441 gatcacggac cgcaacctcc cgctttcggc ttacctgggc gtcgtgggta tgcccggcgt
     4501 gacggcgtat gtcgggttgc tcgatattgg gcagccgaag agcggcgaga cggtggtcgt
     4561 gtcggccgcc ggcggcgcgg tcggcggtgt agtcggacag atcgccagga ttcacggttg
     4621 tcgcaccgtg ggtattgccg gcgggccgga aaaatgccgc tacgtagtcg aagaattggg
     4681 cttcgatgcc tgcgtggact acaaaacggg ccggttgtgg gaagatgtga aggaagccac
     4741 gccccagggt atcgacgtct acttcgacaa cgtgggtggc gaaatactgg ataccatcct
     4801 gcgccgcatg aacccgttcg ggcgcatccc gctgtgcgga gtgatctcgc agtacaacgc
     4861 cacccagccg tacggcgtga aaaacac
//
LOCUS       DBNHXB010000003        20414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_503897, whole genome shotgun sequence.
ACCESSION   DBNHXB010000003 DBNHXB010000000
VERSION     DBNHXB010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 20414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20414
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_503897"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="AC162G_00075"
     CDS             complement(<1..238)
                     /locus_tag="AC162G_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006909340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HIR5074241.1"
                     /translation="MDAQSVQLSHEEIRRYSRHLIMPEVGMAGQKKLKAASVLTVGAG
                     GLGSPLAMYLAAAGVGRIGVVDFDVVDESNLQRQI"
     gene            complement(378..953)
                     /locus_tag="AC162G_00080"
     CDS             complement(378..953)
                     /locus_tag="AC162G_00080"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025105.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR5074242.1"
                     /translation="MPERILIAVALIGLGVLAYRLLLSAQRRRATSAAGRGRTPSAFL
                     RLPLRLRFPLPLPFPSLRVGVGDLAGQGSGQALRGQATNDGRPALIVFTSPTCAPCKL
                     QQMPVVDRLMAGWAERIDLRVVDVTEQPDVAARFGVWSLPTTIVLDAGGRVSALNQGV
                     ASEQKLREQFERVSGWATNQRMGGPANQPAH"
     gene            complement(957..1409)
                     /locus_tag="AC162G_00085"
     CDS             complement(957..1409)
                     /locus_tag="AC162G_00085"
                     /inference="COORDINATES: protein motif:HMM:NF025698.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HIR5074243.1"
                     /translation="MNERKVDHSALRTNQAFIIGLLILAFVFDVWPLVALVAAVMLGG
                     TIWPSAALFKAVYLYVLRPLKIARPDMRVDNPEPHLFAQGVGGAVLTLATIAFAVGAP
                     AAGWILAWVVVALAALNLFAGICVGCMMYYWFNRLGVPGFKASPIPRK"
     gene            complement(1577..1888)
                     /locus_tag="AC162G_00090"
     CDS             complement(1577..1888)
                     /locus_tag="AC162G_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HIR5074244.1"
                     /translation="MNVSIVIPTPLRQYAGGQKVVTVNGVTVGQALDALTTTHPDLKR
                     HLFSEDGKLRSFVNVYLGDEDVRHLQQSETPLMAGATLSIVPSVAGGGNELPSAQRIN
                     G"
     gene            complement(2008..2445)
                     /locus_tag="AC162G_00095"
     CDS             complement(2008..2445)
                     /locus_tag="AC162G_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR5074245.1"
                     /translation="MSLVLSDMQLDAIKQHGQSTYPEEGGGLLLGHAENGRTIVKEVR
                     AIPNTWETNAERRRRYLIPGDLIWRVEEPAAEARGLEIVGYFHSHPDHPAVPSEFDRE
                     HALPNWSYVIVSVQQGRAGDVLAWRLREDRSAFEPQSIAIDDK"
     gene            complement(2459..3847)
                     /gene="cysS"
                     /locus_tag="AC162G_00100"
     CDS             complement(2459..3847)
                     /gene="cysS"
                     /locus_tag="AC162G_00100"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR5074246.1"
                     /translation="MALRLYDTYTRSLRAFEPLHPPEVGLYACGLTVYDYAHLGNLRT
                     YIFEDILRRVLEFDGYVVKHVQNITDVGHLVSDADTGADKMEKGSLRTGRSAWEIAEL
                     YTQVFKDDLRRLNVLEPAIWCKATDHIAEQIEFIRCIEAKGFTYRTSDGIYFDTSKLP
                     EYGYLARLDVEGLKAGARVDAAEKRNPTDFALWKLSPPGQSRQMEWNSPWGVGFPGWH
                     IECSAMAIRYLGAFFDIHCGGEDHIPVHHTNEIAQTQVCYGTRLANFWLHGTFLQVDE
                     ARMSKSSGEFLQLQSLIDRGVDPLAYRYFCLGAHYRSRLSFTWESLDGAATALNRLRM
                     AAHEMGEPGQVDEGYAARFAAEVNDDLNTPRALALAWDLVRSDLPAPVKKATLLRFDQ
                     VMGLRLAEWQPAVTVVPDDIMALVQQRQQARSAKQWQDADTLRDQIVAAGYEIEDTTD
                     GPRVRSRSPVAV"
     gene            complement(3967..4905)
                     /locus_tag="AC162G_00105"
     CDS             complement(3967..4905)
                     /locus_tag="AC162G_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR5074247.1"
                     /translation="MIATLPVLNRLAVQTDLLDLVGNTPLIPLRRVLSARPGVELYAK
                     AEWFNPSGSVKDRPAREIILDAEQSGDLREGVRLLDATSGNMGIAYATLCAARGYEVE
                     LAIPANASPERIAILRTLGASLILTDPMDGTDGAQLEAKRRYAEDPEQYFYADQYNNP
                     ANWQAHYRTTGVEIWEQTDGRVTHFVAGLGTSGTMMGTGRRLREFNLNVQLVSIQPDS
                     PLHGLEGLKHMPTALCPGIYDEGLADENLEIATEDAFAMARRLAREEGLFVGVSAAAA
                     VVAAVRVASGLREGVVVTILPDAGFKYVSERFWKDE"
     gene            complement(5193..5600)
                     /locus_tag="AC162G_00110"
     CDS             complement(5193..5600)
                     /locus_tag="AC162G_00110"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR5074248.1"
                     /translation="MSLHKNKRLWQKHVLAENRRSIQGLLDTLCDEPVYTIMATGEEY
                     RGKEAVAAFYSGLFEAVPDATFDLINAFVAEDGVVEESVLRGTHQGKWMGAEPSGRKI
                     DLPLTIVFPMKNGQILGERMYFDMGTLMRCLGK"
     gene            complement(5775..6425)
                     /locus_tag="AC162G_00115"
     CDS             complement(5775..6425)
                     /locus_tag="AC162G_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074249.1"
                     /translation="MERKAPFQAAVLAAIVAFVCAILLGFVFRAPDPGVSLQPVTPGP
                     AAEFVRPINDYPELMLRFLASDTLFVLSYLVVFAGLYATVADRARVVAIVGLGAGVLT
                     ALFDATENAYFVTYAAASLNGVPLTDPDVSTVYVLANLKWAGAFATLYAFGLAWPRGD
                     RLGWIISALMLLFVLVGVLGIAVPGLIPLRGIFFLAGMPLFAWHFWRQSQRVKRDV"
     gene            complement(6558..9938)
                     /locus_tag="AC162G_00120"
     CDS             complement(6558..9938)
                     /locus_tag="AC162G_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5074250.1"
                     /translation="MHKARHILVAIAVVIGLLMPAAALAQDGAPPRRGLAAVNDEGVT
                     VMVRSGEQISGTIGVVVYLNKPSLARAGEHMTPARRATYAASVSALQNDVAAKIEALG
                     GAIVGRFETLSSGLAVSIDAAKIGDLRNIREVVTVRKAIDYQLELGEAVPWIGAADVQ
                     ALGVDGEGVDVATIDTGADYTHVKLGGPGTAEDYIDAYCGPSFDPTVDGPVDPTNPAC
                     AAHAAKDMTGLFPGSKIVGGYDWLGDLWPVGSTTISPDPNPIDFHGHGTHVADIIAGL
                     PGGGIGPGVAPGANLWTFKACSSLSSACNGLSVLLAMDDAADLDDDPKTTDPADVINL
                     SLGAIYGQPEDDLTAFANEATLIGAIVVAGGGNSGDKPFIVAPPSTAIGAIGVAWMSA
                     PTEKLVRIDTSTADAHGRWQTWSAPQTATVSGTLFYDTTSDFTRRGCIHDTLGNHLGS
                     PWTPGQHAGRVLFMDRGGCAVSHKVSNAAAAGAVLAIIANNVPQAPHEPPPDFIFSGG
                     TPSIPGYTITLADADALKATALGDSATVDPAMAVSLQDSLVSSSSRGPRNHDNAIKPD
                     IAAPGTWVSARVGTGSSTTAFGGTSGASATVAGAAALLKQKYGSLELPASIAAELGGE
                     MPVYMYKALLMNTASTEVYLGVRDFGAPLAPITVIGGGRLDMAGALDAQTLAWDETDR
                     PIDSRFPTGDDDDCDDGEEDDETDCPIDPRFRTGSMSFGYQAITRREVAVRRGVVANL
                     SDEGRWYDLSASFRYAEDENRGVSLKVYPKRLYVPAGSYRAFFVKMHLDAETLRDWPF
                     LTLDAVDKGFNGANGGALTRQEYDGTIHIDGGEYNTVHLVWHVLPKRVAAIRPMRHTL
                     GFGQHVTRTLTLHNSAMFQDGDVDVFSLVDLNPNTYFYTVLDDIGFINECMSGPPLSR
                     GHPGPGCNETPIDIKEVGVRDFSDTFVEFGLTIWDSPYRSSQVPVEFDIFVDSNLDGN
                     NDWVVLNYPFNSNLSDGRNVVWVCQPDFTDCTIPFFVDSGFNTQNWILAVPAAAIGVA
                     PGQQFNFQVRARDFYFTGEYTDVSPADGVSYHTYTMGRPRYTVDNLLPVVPAEGETPL
                     TVTRSAEGEAASPSQIGLLLMYRQAPVERESDSVLIKP"
     gene            complement(10026..11012)
                     /locus_tag="AC162G_00125"
     CDS             complement(10026..11012)
                     /locus_tag="AC162G_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074251.1"
                     /translation="MACAPTTPTPGAGPIIEVIEDTFRMPALITPLSSPLAPPDVRPS
                     VGGTQAGIPAASPTFAAGVSQLPANTAAPNAEPAATPAVPPAAPDGQTPLPRLSPTVP
                     PAAATGNAPATLIRETAQSGATPSPSATLSPGATTTGSPLAIRNDQSCPDTEGGVLYI
                     FGEIVNSSSQFYDIDDIEVTIYGLTGVIPLLHESLDMPGDYFVPPNNTMPFQITAQLE
                     QLDFIDYNLSISAEPSQHSPRDDLTIELAGTTEEEGDLLVDVTWTNPSLVSTYVAPFV
                     VAYDAQRRVSNLAYEPLAGSHTYTGTHTIEMTLYANPCWSADDMLVTGIVGE"
     gene            complement(11587..14553)
                     /locus_tag="AC162G_00130"
     CDS             complement(11587..14553)
                     /locus_tag="AC162G_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR5074252.1"
                     /translation="MNGETQVTLRVAHRLRRRAANKSSKNIPKLLAGCLALLVSLCAL
                     TFFGSIGAAAAVYTSLTQDLPDPSQIEAEFTQGSDEFFETTKIYDRTGQVLLYEVIDE
                     SAGDRQWVHLDQIPEICQNATIAIEDRTFRTNVGFDILGITRAFVNNLRGLPVQGASS
                     ITQQVVKNTLIDPEERYAQSYERKIKEAILAVEISRRYSKEQILEWYLNTNNYANLAY
                     GIEAAAQVYFGKSTGELNLAECAMLAPIPQFPRLNPIDSPLDAKERQAIVLDAMLREG
                     YLTPEEAVAAKFQDLQISGGVAERFDIQAPHFSVYVRKWLEEHFGYETVNRGGLNVYT
                     TVDMDLQNFAQKAIASQVTELDAEGHNVDNGALVAIRPSTGEILAMVGSADYWNDAIN
                     GKFNVASGLRQPGSSFKPFTYVTLLSQGYPASYAFLDVPTRFDQPGANPPVYVPENYD
                     RKYHGYQRMRLALARSYNIAAVQALRLAGVDNVIRLAHKMGITTLDRGLDFYGLALTL
                     GGGEVKLLDMVYAFSVFATTGSMNGTPVLEERPGYRKLDPVPVLRVEDSNGNVLWQYS
                     AGAVYNGQETMQTQPILDAPLAYLMNSILSDNAARWAAFGSPNVLELDRPAAVKTGTT
                     NDFKDNWTLGYTPQIAVGVWIGNTDNTEMKNVSGITGAAPVWHDVMAYYLKDKPVEPF
                     VRPPGLVEKTVCATSGLLPTKYCPTTTELFIPGTEPTSYDTLHQAFLIDKATGKLATV
                     FTPPDQVEEKIFEIYPPEAAEYVASAEIPQPPSEYDTVFGPPPNAYGDVAIIDPGPYA
                     YIRGQTVITGNAKIGNFASYRLDFGQGLNPTQWQQIGPDHTNPVDQGPLELWDVSSSD
                     GLYSLRLTVFQNDGNAATSVVQVTVDNISPTLKLIYPPDNEEYFFGNDEWISLNPDVQ
                     DNIAIDRVEFFVDDEQTPFLVRPVPPYDAKWLLNDGKKMGNHTFYARAYDKAGNVIES
                     NKVRVAVRPRPPEDSP"
     gene            complement(14682..15464)
                     /locus_tag="AC162G_00135"
     CDS             complement(14682..15464)
                     /locus_tag="AC162G_00135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5074253.1"
                     /translation="MTSSRITPHTMSTGGSQPGIKQSKSALRGEPSYVWRFGQNRRLD
                     MILRALPLDARTILVDGCGIGTYVKHLADLGYNTFGLDVDLERVAQGSGAGAEQLHVA
                     AGEHLPYADAAFDAVLSHEVVEHVADDRLAAREMVRVLRPGGRAIVFCPNRWYPFETH
                     GHFWRGQYHFGNTPLINYLPNPIRDRLAPHVRVYTGRGLRSLFTGLPVRVVVHTQIYP
                     GYDNVAARRPALGKALRAVTYAFERTPLHAFGLSHFLVLEKT"
     gene            complement(15518..16450)
                     /locus_tag="AC162G_00140"
     CDS             complement(15518..16450)
                     /locus_tag="AC162G_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5074254.1"
                     /translation="MQFLVTGGAGFLGTHLCNRLARDGHTVRALDDLSAGDPSRLAAS
                     VLFTRGDVADIPKLWSLLQGIDCVYHLAARVSVPQSILYPRDYNAVNVGGTVALMEAV
                     RDAGVKRVVLASSGAVYGQQAAQPVREDMPPYPDSPYAVSKLSAEFYVRTIGALWNIE
                     TVILRIFNAFGPGQAVPVSHPPVVPQLLKQATGGGSLVVFGDGTQTRDFVFVDDVVDA
                     LVAAATAERVAGEVINVGSGQETSIVDLIRHIEQVVGRQVEPLHNSSVSGGVRRLVAD
                     ITKARRLLNFQPRISLDEGLRQTLSRDLNYRPDR"
     gene            16659..16961
                     /locus_tag="AC162G_00145"
     CDS             16659..16961
                     /locus_tag="AC162G_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024139.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5074255.1"
                     /translation="MVKSKPVIEPNAVYSREEAAEILGVSLSTVKRMIAEGYLQVSRL
                     NGGRRLFIRGVNILAMLDQATVQNQTSRKPALSRSPDPERSEGDRAGKGSKRALKK"
     gene            17150..18550
                     /locus_tag="AC162G_00150"
     CDS             17150..18550
                     /locus_tag="AC162G_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074256.1"
                     /translation="MRLWRQWRPLLQQVFERSLTLGVIAFFLVGDVLPSASFDAQLTR
                     AVGADRFDFLGWELQSFGEKGAQAALPIQAYLDEKQRAQVVVDYLELSRRLDGVERDI
                     NRMYADPDVSDPESASAAQRAERDRLRADVERRRPTAEAIVQDQIAAVLADEGLAAGG
                     RVFPPVLARITPLPNILVISPRDEIKREPGMGLSAGLGIDRADEIEDALFESTDKSAL
                     VTPIGGLSLYPSMIVETTDLLGLLQVVGHEWVHHWLFFRPLGIELLLTSIAGGDVLTM
                     NETVASLVGDEAGILALKRFYPEIAKRDYPFVYEPPKPVEDPDAPPSESDPNAFNFNR
                     EMHETRVQVDEYLATARELKRKAGEAAAAGRDEEAATLRSEALDWIVRAETYMEDRRK
                     LFVENGYWWIRKLNQAYFAFYGSYADRPGASGEDPVGPAVRELRAKIPRIADFLNAVA
                     PVTSFDELKRVLEQYP"
     gene            complement(18551..19687)
                     /locus_tag="AC162G_00155"
     CDS             complement(18551..19687)
                     /locus_tag="AC162G_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR5074257.1"
                     /translation="MNDLLDACTRRLPDALALLKQLVALESPSTDKAAVDRCSAFTEA
                     KFRELGLATQRIPSATAGDQLIADWPGGDGRSGRVLALLHLDTVWPIGTLERMSIREE
                     NSRLYGPGAYDMKGSVAIAWQAIGALRDSGRAPRRPLRLLFTSDEEIGSNASVDLIRA
                     EALQSDLVLVMEPALPNGELKTARKGVGGFKIVAHGRSSHAGGNHDKGVNAIEELAHQ
                     VLALQKLTDYERGTTINVGVIAGGTRSNVVPDRAEIDVDFRAASMAEAERVRQAFQSL
                     RPVLPEARLEVSGGLNRPPMERDATMIATFEKARRIGAAIGLELKEGSTGGGSDGNFT
                     AALGIPTLDGLGPLGDGAHALHEHVVIESLVRRAALLAALWTQW"
     gene            complement(19762..20304)
                     /locus_tag="AC162G_00160"
     CDS             complement(19762..20304)
                     /locus_tag="AC162G_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074258.1"
                     /translation="MSISQSTWNALKNVTIVMSLAINIVLIVVVLILIGQIGAIKATL
                     NGVLTQLDMAFSSLGAAVIQDTIHIDQRVPVRFDLPVDQNVTAVTTAPVPLAIPASFS
                     LGSFGHINGTVSLSLPQNLSLPIRISMVVPVSNEIPVVFDQPVAIPLGQKGLGPVINQ
                     LRGVTQPLIQLVDAIPDSIP"
BASE COUNT         3782 a         6281 c         6507 g         3844 t
ORIGIN      
        1 tgatttgccg ctgcaggttg ctctcgtcga cgacgtcgaa gtcgaccacg ccgatgcgcc
       61 ccacgccggc cgccgccagg tacatcgcta gcggcgatcc cagcccgccc gctccgaccg
      121 tgagcacgct ggcggccttg agtttcttct gccccgccat gccgacttcg ggcatgatca
      181 ggtgacggct gtaacgccgg atttcttcgt gacttaattg aacggattgt gcgtccatcg
      241 cgctccccct gctatatggt cgttacactg cccgcactgg tcgaagcttc cgattggagt
      301 cgaggcttta gccggatccg cctgaaggct cgactccggt cctcggaagt ctggcgtgcg
      361 ctgtgtataa cagttcgcta atgggccggt tggttcgccg gcccgcccat ccgttgattc
      421 gttgcccatc cgctgacacg ttcgaactgc tcgcgcagct tctgctcgct cgccacacct
      481 tgattgagcg ccgacacacg gccgcctgcg tccagcacga tcgtcgtcgg cagcgaccac
      541 acgccgaagc gcgcggcgac gtcgggctgc tcggtcacgt cgacgacgcg cagatcgatg
      601 cgctcggccc acccggccat caaccggtcg acgacgggca tttgctgcag cttgcacggc
      661 gcgcaggtcg ggctggtgaa gacgatcagc gcgggtcgtc cgtcattcgt cgcctgccct
      721 cgtagcgcct gtcctgagcc ttgcccggcg aggtccccga ccccgaccct gagcgaaggg
      781 aaggggagag ggagtgggaa gcgcagtcga aggggcagcc gaaggaaagc ggagggggtt
      841 cgcccgcgcc ctgcggcgga cgtcgcccgt cgtcgctgag ccgagagcag cagccggtag
      901 gcgagcacgc ccagcccgat caacgcgacc gcgatcagaa tacgctcagg catagatcac
      961 ttccgtggaa tcggcgaggc cttgaagccc ggtacgccga gccggttgaa ccagtaatac
     1021 atcatgcagc cgacgcagat gccggcgaac agattcagcg cggccagcgc cacgacgacc
     1081 cacgccagga tccagccggc ggcaggcgct cccacggcga aggcgatcgt cgccagtgtc
     1141 aagactgccc cgcccacgcc ctgcgcgaac aggtgcggct cgggattgtc cacgcgcatg
     1201 tcaggtcgcg caatcttcaa tggcctcaga acatacagat acacggcttt gaacagcgcc
     1261 gcgctcggcc agatggttcc gcccagcatg acggctgcga cgagcgccac gagcggccac
     1321 acatcgaaca cgaacgccag aatcagcagc ccgatgatga acgcctggtt cgtccgcagt
     1381 gcggaatgat cgaccttacg ctcattcata atctcgtccc cttccatgac accattaact
     1441 ggacgaccgc aagagtcgct aagagcctat ccgaaaacgt agcctggccc ctcgtgggcg
     1501 gcgggcacaa ggcccgatgc tacggggttt tcggataggt actaaatcac gtatccacca
     1561 cagcccggtc gtcgactcat ccgtttatcc gttgtgccga aggcaattcg ttgcccccac
     1621 cggccaccga cggcacgatg ctcagcgtcg cgcctgccat cagcggcgtt tcgctttgct
     1681 gcaagtgtcg cacgtcctcg tcgcccagat agacgttgac gaaagagcgc agctttccgt
     1741 cctcgctgaa caggtgacgc ttcaggtcag ggtgcgtcgt ggtcagcgcg tcgagcgcct
     1801 ggccgacggt gaccccgttc accgtcacta ctttctggcc gccagcgtac tgtcgcagtg
     1861 gggttgggat gacgatgctc acgttcatag cctgtatctc ctcaccttga agataatgat
     1921 gtcgctcgcc aggggtctgc gaccccggcc ccttggccgc cgggcgacac acccggccgg
     1981 agcagttgtg ctttcagtcg tcgccgatca cttgtcgtcg atcgcgatcg attgcggctc
     2041 gaacgcgctg cgatcctcgc gcaaacgcca cgccagcacg tcgccggccc ggccctgctg
     2101 caccgagacg atgacgtagc tccagttggg cagcgcgtgc tcgcggtcga actccgacgg
     2161 cacggccgga tgatccggat gcgaatggaa gtagccgaca atttcaagcc cgcgcgcttc
     2221 ggccgcgggt tcctccacac gccaaatcag gtcgccagga atcaggtatc gccgccgtcg
     2281 ctcagcattt gtttcccagg tgttgggaat agcccggact tccttcacga tcgtgcgccc
     2341 gttttcggca tgccccagta gcagcccgcc gccttcctcg ggatacgtag attgcccgtg
     2401 ctgcttgatg gcgtcgagtt gcatgtcgct caaaaccagg ctcatgcgag tcccttcctc
     2461 agacggcgac gggcgatcgg ctccgcacgc gcggcccgtc cgtcgtgtcc tcgatctcat
     2521 agcccgccgc gacgatttga tcgcgcagtg tatcggcgtc ctgccactgc ttcgccgagc
     2581 gcgcctgctg ccgctgctgc acgagcgcca taatgtcgtc ggggacaacc gtcaccgccg
     2641 gctgccattc ggccaggcgc aagcccatca cttgatcgaa gcgcaggagc gtcgccttct
     2701 tgaccggggc aggcaggtcg ctcctgacca ggtcccaggc cagcgccagc gcgcgcggtg
     2761 tgttcaggtc gtcgttgacc tctgcggcaa acctggcagc gtaaccttcg tccacttgac
     2821 ccggctcgcc catctcgtgc gccgccatgc gcagacgatt cagcgccgta gccgccccgt
     2881 ccaggctttc ccaggtgaag ctgagacgcg accggtaatg cgcgcccaga cagaagtaac
     2941 gataagccag cggatcaact cctcgatcga tcagcgactg cagttgaaga aactcgccgg
     3001 acgacttgga cattctcgct tcgtcgactt gaaggaaggt gccgtgcagc cagaaattcg
     3061 ccaggcgcgt gccatagcac acctgcgtct gcgcgatctc gtttgtatgg tgcaccggaa
     3121 tgtggtcctc gccgccgcag tgaatgtcaa agaacgcgcc caggtatctg atggccatcg
     3181 ccgagcactc gatatgccaa ccggggaagc cgacgcccca cgggctgttc cactccatct
     3241 gccggctctg gccaggcggg ctgagcttcc acagcgcgaa gtcggtcggg ttacgcttct
     3301 ccgctgcgtc cacccgcgcg ccggccttaa gcccttcgac gtccagccgc gccaggtagc
     3361 cgtattccgg caatttggac gtatcgaagt aaatcccatc tgacgtgcga tacgtgaagc
     3421 ctttcgcctc gatgcagcgg atgaactcga tctgctccgc gatgtgatcg gtcgccttgc
     3481 accagatcgc cggttcgagc acgttcagcc gccgcagatc gtctttgaag acctgcgtgt
     3541 acagctcggc gatctcccag gccgaccggc ctgtgcgcag cgatcccttc tccatcttgt
     3601 ctgcgccggt gtcggcatcg gacaccagat ggcccacgtc ggtgatgttc tggacgtgct
     3661 tgacgacgta gccgtcgaac tccaatacgc ggcgcaagat gtcctcgaag atgtacgtcc
     3721 gcaagttgcc gaggtgggcg tagtcatata cggtcaggcc gcacgcatac aagccgacct
     3781 ccggcggatg cagcggctcg aaagcgcgca ggctgcgggt ataagtgtcg tatagacgca
     3841 gagccatggg tgtcctgaag ttaccagtgg ccagtaatca gtaatcagcg atcagtatgc
     3901 tgacgtcgat agcctgttac tgattgaccg ttcaccgtta actgattact ggtcactgat
     3961 tactgatcac tcgtccttcc agaagcgctc actcacgtac ttgaatcctg catccggcag
     4021 gatcgtgacg acgacgcctt cgcgcaggcc tgacgccacg cgcacggcgg cgacgaccgc
     4081 cgctgccgcc gacacgccga cgaagagacc ctcctcacgc gccaaccgcc gcgccatggc
     4141 gaaggcatcc tccgtcgcga tctcgaggtt ctcatccgcc agcccctcgt cgtagatgcc
     4201 ggggcacagc gcggtcggca tgtgcttgag accttccagt ccatgcagcg gcgaatcggg
     4261 ttgaatcgag acgagctgca cattcaggtt gaattcgcgc aggcggcgcc cggtgcccat
     4321 catcgtcccg ctcgtgccca agccggcgac gaagtgcgtc acgcggccat cggtctgctc
     4381 ccagatttcg acaccggtcg tgcgatagtg cgcttgccag ttggccggat tgttgtactg
     4441 gtcggcataa aagtactgct cggggtcctc ggcgtagcgc cgctttgcct ctaactgcgc
     4501 gccgtccgtg ccgtccatgg gatccgtcag aatcaggctc gcgccgagcg tccgcaggat
     4561 ggcgatgcgc tccgggctgg cgttggccgg gatggccagc tcaacctcgt agccgcgcgc
     4621 cgcacacagc gtggcatagg cgatgcccat gttgcccgac gtcgcgtcga gcagcctgac
     4681 gccttcacgc aggtcgcccg actgctcggc atccaggatg atctcccgcg cggggcggtc
     4741 tttcaccgag ccgctcggat tgaaccattc tgccttggcg tacagctcga cgccgggccg
     4801 ggccgacagg acccgccgga gcggtatcaa gggcgtgttg ccgaccaagt cgagcaggtc
     4861 ggtttgaacg gcaagtcgat tgagaactgg aagggttgcg atcatcattg cctcccggcc
     4921 ggggcgccgc gaggacgagg gcaatggcga tgcgcaggag cgcggttagt ggcaaacaaa
     4981 aagaccaacg tgtgcgccag tacgcaacgt gactatgctt ctcccccgcc tcgcggaagc
     5041 acagccactg gccttcgtcg gtctcatgta tgaggtaaac gaggcggcgg ctagcgcttc
     5101 cgcctcaaac acgtgcaaaa ggacactacc aacctcaccc tggtaagctg ctcaacatta
     5161 tatccataat gacgacgcgc gtcaagtaaa tcttacttgc ccaggcaccg catcagcgtg
     5221 cccatgtcga agtacatccg ttcgcccaga atctggccgt ttttcatagg aaacacgatg
     5281 gtcagcggca gatcgatctt gcggccagac ggctcggcgc ccatccattt gccctgatgc
     5341 gtgccgcgca gcaccgattc ttcgaccacc ccgtcctcgg cgacgaaggc gttgatcagg
     5401 tcgaacgtcg catcgggcac cgcctcgaac agtccgctgt agaatgcggc gaccgcctct
     5461 ttgcctcggt attcttcgcc ggttgccatg atcgtgtaca ccggctcgtc gcacagcgtg
     5521 tcgagcaggc cctggatgct gcggcggttc tcggccaaca cgtgtttttg ccaaaggcgc
     5581 ttgttcttgt ggagcgacat ggagagtcct ttcctggccg gttgtcgatt ctcagaaact
     5641 gtctcaaaac ctaccactaa gacacgaaga caccaagaaa agccttgaaa gccttcgtgt
     5701 cttggtgtct tcgtggtcaa taaaggcctt tttaagacgc gttgtcagga tgcgtcccgg
     5761 actttcatca ctcgtcacac atcacgcttc acccgttggc tctgccgcca gaaatgccag
     5821 gcgaacagcg gcatgccggc caggaagaat atgccgcgca gcggaatcag gccgggcacg
     5881 gcgatgccca acacgccgac gagcacgaac agcagcatga gggcggagat gatccagccc
     5941 aggcggtccc ccctcggcca ggcaaggcca aaggcataca gcgtggcgaa agcgcccgcc
     6001 cacttgaggt tggcgaggac gtaaacggtg gacacgtcag ggtctgtgag cgggacgccg
     6061 ttgagcgagg ccgcggcgta cgtgacgaaa tacgcgttct ccgtcgcgtc gaacagcgcg
     6121 gtgagcacgc ccgcgcccag gccgacgatc gcgacgacgc gcgcgcggtc ggcgaccgtc
     6181 gcgtacaggc cggcgaacac gacgaggtaa ctcagcacga acagcgtgtc gctggccaga
     6241 aaacgcagca tcagctccgg atagtcattg atggggcgga cgaactcggc tgccggaccc
     6301 ggcgtgaccg gttgaaggct cacgccggga tcgggcgcgc gaaaaacgaa gcccagcaag
     6361 atcgcgcaaa cgaaggcgac gatcgcggcg agcacggcgg cctgaaacgg tgcttttctc
     6421 tccatctggc ggcagtcctt tcctggcaga caatcaccac agagttatcc gtgcgcctgt
     6481 ggcgcgcccc gaccgatgaa aacacaccgc tcacgccggg tctgtgaccc gacgtaggta
     6541 tacggggtca aggatgacta tggcttgatc aacacagagt cggactcgcg ctcgaccggc
     6601 gcctggcgat acatcaggag caaaccgatc tgcgagggcg acgccgcctc gccttccgcc
     6661 gatcgggtga ccgtcagtgg ggtctcgccc tcggccggga cgaccggtaa cagattgtcg
     6721 acggtgtatc tcggccggcc catggtgtaa gtgtgatagc tcacgccgtc ggccggcgag
     6781 acgtcggtgt attcaccggt gaagtagaag tctcgggccc gaacctgaaa attgaactgt
     6841 tgcccaggcg ctacgccgat ggccgcagcc ggcacggcca ggatccagtt ctgagtgttg
     6901 aagcccgagt cgacgaagaa gggaatcgtg cagtccgtga aatccggctg gcaaacccat
     6961 accacgttgc ggccatccga caagttgctg ttgaacgggt agttcagcac gacccagtcg
     7021 ttatttccgt ccaggttgga gtcgacgaaa atgtcgaatt cgaccggaac ctggctggat
     7081 cgatacgggc tgtcccatat tgtcaggccg aattcgacga aggtgtcgga aaagtcacgc
     7141 acaccgactt ccttgatgtc gatgggcgtc tcattgcagc cgggaccggg atgcccgcgc
     7201 gagagaggcg ggccgctcat gcactcgttg atgaagccaa tgtcgtcaag tacagtgtaa
     7261 aagtaagtgt tcgggtttag gtcgacgagt gagaacacgt caacatcgcc atcttggaac
     7321 atggctgagt tgtgcagcgt cagggtccgc gtgacgtgct ggccgaagcc cagcgtatgc
     7381 cgcatcggcc ggatggccgc gacgcgttta ggcaagacgt gccagacgag gtggacggta
     7441 ttatactcac cgccgtcgat gtggatggtg ccgtcgtatt cctggcgggt gagcgcgccg
     7501 ccattcgctc cgttgaagcc cttgtccacc gcgtcgagcg tcaggaaggg ccagtcgcgc
     7561 agcgtctcgg cgtcgaggtg catcttgacg aagaaagcgc gatatgagcc ggccggcacg
     7621 tacaggcgct tgggatacac tttaagactg acgccacgat tctcatcttc ggcatatcgg
     7681 aacgatgccg acagatcgta ccagcggcct tcgtcgctca ggttggccac cacgccgcga
     7741 cgcacggcaa cctcgcgacg ggtgatggcc tgatagccga aagacatgct gccggtgcgg
     7801 aaacgagggt cgatcgggca atctgtctcg tcgtcctctt cgccgtcatc gcagtcgtcg
     7861 tcatccccgg tggggaagcg ggagtcgatc gggcgatctg tctcgtccca ggcgagcgtc
     7921 tgggcatcca acgcgccagc catatcgagg cgcccacccc cgatgacggt aatcggcgcg
     7981 agcggcgcgc cgaaatccct gacgcccagg tacacctcgg tgctggcagt gttcatcagg
     8041 agcgctttat acatataaac cggcatctcg ccgccgagtt cggcggcaat cgacgcgggc
     8101 agctctaagc tgccgtactt ctgcttgagg agggccgccg cgccggcaac cgtcgcgctt
     8161 gcgccagagg tgccgccgaa ggcggttgtg ctgctaccgg tgccgacacg cgccgagacc
     8221 catgtacccg gcgcggcaat gtccggcttg atagcattgt cgtgattacg cggcccgcgc
     8281 gacgaggacg agaccagcga gtcttgcaat gacacggcca ttgccggatc gactgttgca
     8341 ctgtcgccga gagcggtcgc cttgagcgcg tcggcgtcgg ccagcgtgat cgtgtagccc
     8401 gggatgctgg gcgttccacc gctaaagatg aagtccggcg gcggttcatg aggggcctgc
     8461 ggcacgttgt tcgcgatgat ggccaacacc gcgccggccg ccgccgcgtt tgacaccttg
     8521 tggctcacgg cgcagccgcc gcggtccatg aagagcaccc tgccggcgtg ctggccgggt
     8581 gtccacgggc tgcccaggtg gttgcccagt gtgtcgtgaa tacagccacg ccgggtaaag
     8641 tcgctagtcg tgtcatagaa cagtgtgcct gagacggtcg ccgtctgggg cgccgaccac
     8701 gtctgccacc gcccatgcgc atcggcggtg ctcgtgtcaa tcctgactag cttctcagtt
     8761 ggcgcgctca tccacgccac gccgatcgcg ccgatagccg tcgagggcgg tgccacgatg
     8821 aacggcttgt cgccggagtt gccaccgcct gccacgacga tggcgccgat gagtgtggct
     8881 tcgttggcaa aggccgtcag gtcgtcctcg ggctggccgt agattgcgcc taacgacagg
     8941 ttgatcacgt cggccgggtc agtggtcttc ggatcgtcgt cgagatcggc ggcatcgtcc
     9001 atggccagca agactgaaag tccgttgcac gccgacgaca gcgacgagca agccttgaat
     9061 gtccagagat tcgcgcccgg cgcgacgccc ggcccgatgc caccgccggg caggccggcg
     9121 atgatgtcgg cgacgtgcgt gccatgacca tggaagtcaa tcgggttagg gtcgggcgag
     9181 atggtcgtgc tgccgaccgg ccagagatcg ccgagccagt catagccgcc gacgatcttc
     9241 gagcctggaa acaaccctgt catgtctttc gccgcgtggg cggcgcacgc cgggttcgtc
     9301 gggtctacag gcccatccac tgtgggatcg aagctcgggc cgcaataagc gtcgatgtaa
     9361 tcctccgccg tcccgggacc gcccagcttg acgtgggtat agtcagcgcc agtgtcgatc
     9421 gtggcgacgt cgacgccctc gccgtccact cccagggcct gtacgtcggc tgcgccaatc
     9481 cacggcacgg cttcgccaag ctccagttgg tagtcgatcg ccttccgcac cgtcacgacc
     9541 tcgcggatat tgcgaagatc gcctatcttt gccgcgtcga tgctcacagc cagcccactg
     9601 gacagcgtct cgaatcgccc gacgatcgcg ccgcccagcg cctcgatctt cgcggcgacg
     9661 tcgttctgca gcgcggacac cgaggcggcg tacgtggcgc gccgcgcggg tgtcatgtgt
     9721 tcgcctgcgc gcgcgagcga gggcttattc aggtacacaa cgacgccgat cgtcccggag
     9781 atctgctccc cgctgcgcac catcaccgtg acgccttcgt cgttcacggc tgccaggcca
     9841 cgcctgggag gagcgccatc ctgcgcgagc gcggctgcgg gcatcaacag gccgataact
     9901 actgcgatcg cgacgaggat gtggcgcgcc ttgtgcattt gggttcctcc tcggtcagac
     9961 gaccgggtcg cgctggccgg atatggcaca cctgtgtcgc gccgtacgcc gaaggcggtc
    10021 gatggctatt cgccaacgat accagtgacg agcatgtcgt ctgcgctcca gcatggattg
    10081 gcgtacagcg tcatttcgat cgtatgcgtg ccggtatacg tatgagagcc ggctagaggc
    10141 tcgtaagcga gattgctcac gcgcctttgt gcgtcgtagg cgacaacgaa gggcgctacg
    10201 tatgtcgata cgagactggg gttcgtccac gtgacatcga ctagaagatc gccttcttct
    10261 tcggtcgtgc cggccagttc gatagtcaaa tcgtcgcgcg gcgaatgctg gcttggctcg
    10321 gcggaaatgg acagattgta atcgatgaag tcgagctgct caagctgcgc ggttatttgg
    10381 aagggcattg tattgttggg cgggacgaag tagtctccgg gcatgtcgag agactcgtgc
    10441 aggaggggga tgactcctgt cagtccgtag atggtcacct cgatatcatc gatgtcgtaa
    10501 aattgagaag aggagttgac tatctcgccg aaaatgtaca acacgccccc ctccgtatcg
    10561 ggacagctct gatcgttgcg gatggcgagt ggcgatcctg tggtcgtcgc gccgggcgac
    10621 agtgtcgcac tgggcgatgg cgtcgcgccg gattgcgccg tttctcgtat cagtgtggca
    10681 ggggcattgc ctgtggccgc tgccggcggg acggtaggac tgaggcgcgg gagaggcgtc
    10741 tggccgtcag gagcagcagg cggcacagcc ggcgtggcgg ccggctctgc attcggcgcg
    10801 gcggtattcg ccgggagctg agagacgccg gccgcgaagg ttgggcttgc agccggtatc
    10861 cctgcctgcg tgccgccgac cgatggtcgc acgtcaggtg gcgcgagcgg gcttgagagc
    10921 ggtgtgatta acgcgggcat gcggaaagtg tcctcgatga cctcgatgat agggccggcg
    10981 cccggcgtcg gcgtcgtcgg cgcgcacgcc actgcgatcg ctatcagggc gagcgccagc
    11041 gggatgcgga ggcgatgact cccgcagaac gatttgcgat cgaatgctct gcgtgaccat
    11101 agacgcgctc ggctgattgc gtcccgccca gcggggtcgc cggcgcgacg ttcaagagcg
    11161 aagcgcccgg attgctcagc ctcgttgacc cgagagcgcc tgaacagagt gataacagat
    11221 ccaatcggtt tggtccagat cggacacaac cgtacaagtc gcagccgccg tgcggcacag
    11281 cagtcaccga acgatcgtat gggaccggac acaaagtcac gcacattata ggggatgtat
    11341 ggtgaatttt cgggtaagaa ttcgttagag tttgctagat tcacgcgcaa actcgatgcg
    11401 attgaaccca gagcgtcgcg atcaggcatg gtttggtggg attctgacaa gtttgtgcag
    11461 acgaggatac gacttattac ccatcacgtc caagatggtt ggagatgtgt ttgcaatgca
    11521 tcgcccacta gaaaaaggcg cgcccttccg gggcatggaa gggcgctgcg catagcttcg
    11581 cacgagttac gggctgtctt ccggtggcct gggcctcacc gccaccctga ccttgttgct
    11641 ctcgatgacg ttgccggcct tgtcataggc gcgcgcgtag aaggtgtgat tgcccatctt
    11701 ctttccgtca ttcaggagcc acttcgcatc gtacggtggc acagggcgca ccaggaaagg
    11761 cgtctgctcg tcgtccacga aaaactcgac ccgatcgatc gcaatattgt cttgcacgtc
    11821 gggattgagg ctgatccatt catcattgcc gaagaaatac tcttcattgt caggcgggta
    11881 gatgagcttc aaggtcggcg agatattgtc gacagtgacc tgcacgacgg aggtcgccgc
    11941 gttgccgtcg ttctggaaga ccgtcaggcg caggctgtac agcccgtcgg acgacgacac
    12001 atcccacagc tcgagcggac cttgatcgac cgggttcgtg tggtccggtc cgatttgctg
    12061 ccattgcgtg ggattcaacc cttgcccgaa gtcgagccgg taagatgcga agttccctat
    12121 cttggcgttg cccgtgatca cggtctgccc gcggatgtac gcgtacggtc cgggatcgat
    12181 aatggcgacg tcgccgtagg cgttgggcgg ggggccgaag acggtatcgt attcggaggg
    12241 cggctgtggt atctcggccg aggcgacgta ttcggcagcc tcgggtggat agatctcgaa
    12301 gatcttctcc tccacctgat cgggcggcgt gaacacggtc gccagcttgc ccgtcgcctt
    12361 gtcgatcagg aaggcctgat gcagcgtatc gtaggaagtc ggctcagtgc ccgggatgaa
    12421 caactccgtc gtcgtcgggc aatacttggt cggcagaagg ccgctcgtgg cgcacactgt
    12481 cttctcgaca aggccgggcg ggcggacgaa cggctcgacc ggcttgtctt tgagataata
    12541 cgccatcacg tcgtgccaga ccggcgcggc gccggtgatg ccggagacgt ttttcatttc
    12601 cgtgttgtcg gtattgccga tccagacgcc gacggcgatc tgcggcgtgt agccgagcgt
    12661 ccagttatcc ttgaagtcgt ttgtggtgcc ggtcttgacc gcggcaggcc gatccagttc
    12721 cagcacgttt ggcgaaccga acgcggccca gcgcgcggcg ttgtctgata ggatgctgtt
    12781 catcaggtaa gccagtggcg cgtcgagaat cggctgcgtc tgcatcgtct cctgaccgtt
    12841 atatacggcc ccagccgagt attgccacag cacgttgccg ttgctgtctt cgacgcgcag
    12901 cacgggcacc ggatcgagct tgcgatagcc gggtcgctct tcgaggacgg gggtgccgtt
    12961 catgctgccg gtcgtggcga agacgctgaa ggcgtacacc atgtcgagca gcttcacctc
    13021 gccgccgccg agcgtgagcg ccaacccgta aaaatcgagt ccccggtcga gggtcgtgat
    13081 gcccatcttg tgggccaggc ggatgacgtt gtccacgccc gccaggcgca gcgcctggac
    13141 ggccgcgata ttgtacgagc gcgccagcgc cagccgcatg cgctgatagc cgtgatattt
    13201 gcgatcgtaa ttctccggga cgtaaaccgg cgggttagcg ccgggctgat cgaagcgcgt
    13261 cggcacgtcg aggaaggcgt atgaggccgg gtagccctgc gacagtaacg tcacataggt
    13321 gaacggcttg aaggaggaac ccggctggcg caggccggag gcgacgttga acttgccgtt
    13381 gatcgcatcg ttccagtaat cggcgctccc gaccatggcg agaatctcgc cggtagatgg
    13441 acggatggcg accagcgcgc cgttgtcgac gttgtggccc tcggcgtcaa gttccgtcac
    13501 ctggctggcg atggccttct gtgcgaagtt ctgcaggtcc atgtccaccg tggtatacac
    13561 attcaagccg ccgcgattga ccgtctcgta gccgaagtgc tcttcgagcc acttgcggac
    13621 gtacacggag aagtgcggag cctggatatc gaaacgctcc gctacgccgc cgctgatttg
    13681 caggtcctgg aacttggccg ccaccgcctc ttcgggcgtg aggtaaccct cgcgcagcat
    13741 ggcgtcgagc acgatcgcct gccgctcttt cgcgtcgagc gggctgtcga tcgggttcag
    13801 tcgcgggaac tgcgggatcg gcgccagcat ggcacattcg gccaggttca gctcgcccgt
    13861 gcttttgccg aaatacacct gcgccgccgc ttcgatgccg tacgccagat tggcgtagtt
    13921 gttggtgttg aggtaccact ccaaaatctg ttctttgctg tagcgccggc tgatttcgac
    13981 ggccaggatg gcctctttga ttttgcgctc gtatgactgg gcgtagcgct cctcgggatc
    14041 gatcagcgta ttcttgacga cctgctgcgt gatcgacgag gcgccctgca caggaaggcc
    14101 gcgcaagttg ttgacgaagg cgcgggtgat gcctaagata tcaaagccga cgttggtgcg
    14161 gaacgtgcgg tcctcgatag cgatcgtcgc gttctggcaa atctccggga tttgatcgag
    14221 atgcacccac tgccggtcgc cagcgctctc gtcaatcact tcgtacagga gtacctggcc
    14281 ggtgcgatcg tatatcttgg tcgtttcgaa gaactcgtcg ctgccttgag tgaactcggc
    14341 ttcgatctgg ctgggatcgg gcaggtcctg agtgagcgat gtatacaccg ccgccgccgc
    14401 gccgatacta ccgaagaagg tcagcgcgca caggctgaca agcagagcga ggcagccggc
    14461 cagtagcttg gggatgtttt tgctggactt gttcgccgcg cgccgtcgca gccggtgcgc
    14521 cacacgcagc gtgacctgcg tttctccgtt caaaatggtg cgcccctggg gaaactgggg
    14581 cgaaatgtat cagagattgg ccgtttgggc aaggataagg gaagggttcg ctgtcaacga
    14641 atgggaaacg aataaacgac tttttgtcac caccaatgaa gtcaggtctt ctcgagaacc
    14701 agaaaatgcg acagcccaaa ggcgtgcaac ggcgtgcgct cgaaagcgta cgtcaccgcg
    14761 cgcagagctt tgcccaacgc cgggcggcgg gcggcgacgt tatcgtagcc gggatagatc
    14821 tgcgtatgga cgacgacacg gacgggcagc ccggtgaaca gcgaccgaag cccccgccct
    14881 gtgtagacgc gcacgtgcgg cgcgaggcga tcacggatcg ggttggggag atagttaatg
    14941 agcggcgtat tcccgaaatg atactgccca cgccagaagt gtccgtgcgt ctcgaacggg
    15001 taccaccggt tagggcaaaa tacgatcgcg cgtccccccg gtcggagcac gcgaaccatt
    15061 tcccgcgcgg cgagccggtc gtcggcgacg tgctcgacga cttcgtgcga gagcaccgcg
    15121 tcgaaggcgg cgtcggcgta aggcagatgc tctccggcgg cgacgtgcag ttgctcggcg
    15181 cccgctccgg atccctgcgc gactcgttcc aagtcgacat ccagcccgaa tgtgttgtag
    15241 cccaggtcag ccaaatgctt gacgtacgtg ccgatgccgc aaccgtcgac cagaatcgtg
    15301 cgcgcgtcga gtggaagcgc tctcaagatc atgtcgagcc ggcggttctg cccgaagcgc
    15361 cagacatacg aaggttcgcc gcgcaaggcc gatttcgact gtttgatgcc gggttgtgag
    15421 ccgccggttg acatggtgtg tggcgtgatg cgtgacgacg tcatcacgtg attacgtgat
    15481 catgtgatga cgtgatgacg tgatcacgga aggtagatca tcggtcaggc ctgtagttga
    15541 ggtcgcgcga caaagtctgt cgcaggccct cgtcgaggga aatgcgcggc tggaagttga
    15601 gcaagcgccg ggctttcgta atgtcggcga ctagccgccg gacgccgccg ctgaccgacg
    15661 agttatgcag cggttctacc tgtctgccca cgacctgctc gatgtgcctg atcaagtcga
    15721 caatcgacgt ctcttgcccg ctgccgacgt tgatcacttc accggcgacg cgctcggccg
    15781 tcgcggcggc gaccagagca tcgacgacgt cgtccacgaa cacgaagtcg cgcgtctgtg
    15841 tcccgtcgcc gaaaacgacc agggagccgc cgccggtggc ctgtttcagg agctggggga
    15901 ccactggcgg atgcgagacc ggcacggcct gccccgggcc gaaggcgttg aaaatgcgga
    15961 gaatcacggt ctcgatattc cacagcgcgc cgatggtccg cacatagaac tcggcggata
    16021 gcttggaaac cgcgtagggc gaatcgggat agggcggcat gtcttcacgc acaggctgtg
    16081 ccgcctgctg gccgtatacg gctccggacg aggcgagcac gacacgtttt acgccggcgt
    16141 cacgcacggc ctccataagc gctacggttc cgccgacgtt gactgcgttg tagtcgcgcg
    16201 gatagaggat cgattgtggg actgagacgc gcgcggcgag atggtatacg cagtcgatgc
    16261 cctgcagcag cgaccacaat ttcggaatgt cggccacgtc gccgcgcgta aagagcacgg
    16321 aagcggccaa gcgagagggg tccccggcgc tgagatcgtc gagcgcgcgg acggtgtgcc
    16381 cgtcgcgcgc gagccggttg cacaaatgcg tccctaagaa gccggcgccg ccggttacga
    16441 gaaattgcat tccagatcgg tcgagcactg ctatagaaag gtctttctac agcgtacctc
    16501 tacggattat agcacagggt ataccgtgac tgcgcgcccc atgcgatgcc cttgctatga
    16561 accgtcataa cccataataa cctcttaaca accgaaataa accgttgaca atcctaataa
    16621 gccgtgataa actgtttata tctggttggg ggccagagat ggtcaagtcg aaaccggtca
    16681 tcgagccaaa tgcggtgtat tcccgggaag aggccgcaga gattcttggg gtgagtctca
    16741 gcactgtgaa gcgcatgatc gccgaaggct atctgcaggt gagccggctg aacggcggcc
    16801 ggcggctatt tatacgcgga gtgaacatac tggctatgct cgatcaagcg acggtgcaaa
    16861 accagacatc aaggaagcct gccctgagcc gaagccctga ccctgaacga agtgaagggg
    16921 atagggcagg caaagggtcg aagcgcgcat tgaagaaata ggacggtttc cgggtgttgg
    16981 ctcaagacgg cccatcttgt cgaaggtggg cgcttgggcg cggagcgagc ggcgttgaga
    17041 agccgcggct cgctccgacc gattcaggag cctgatcgtc tggcgacgat caggctcctc
    17101 atttgccagt gcctgagtgc cacggtatca tttccgtcaa ggagacatca tgcgcctatg
    17161 gcggcagtgg cgacccctgc ttcaacaggt cttcgagcga tcgctcacgc tgggggtgat
    17221 cgcgttcttc ctggtcggcg acgtcttgcc gtccgcgtcg ttcgacgcac agttgacgcg
    17281 cgccgtcggc gcggatcgct ttgacttcct cggctgggag cttcagtcat tcggcgagaa
    17341 aggcgctcag gctgcgctgc cgatccaggc gtatctcgac gagaagcaac gcgcacaggt
    17401 cgtcgtcgac tacctcgaat tgtctcgacg cctggacggc gtcgagcgcg acatcaatcg
    17461 gatgtatgcc gacccggacg tgagcgatcc cgagtcggcg agcgccgcac agcgcgcgga
    17521 gcgcgatcga ctgcgagccg acgtcgagcg gcgccggccg acggccgagg ccatcgtgca
    17581 agaccagatt gcagccgtgc tggccgacga agggctggcc gccggcgggc gtgtcttccc
    17641 tccggtgctg gcgcgcatta cacccctgcc gaacattctg gtcatttctc cacgcgacga
    17701 aatcaagcgg gagccaggaa tgggcctgtc agccgggttg ggcatcgatc gggccgacga
    17761 gatcgaggac gcactattcg aatcaacgga taagtcggcg ctcgtcacgc ccatcggcgg
    17821 gctgtcgctt tatccatcga tgatcgtcga aacgacggat ttgctgggtc tgctccaggt
    17881 tgtggggcac gagtgggtgc atcactggct gttctttcgt ccgttgggca tagagctgct
    17941 gttgacttcc attgccggcg gagatgtgtt gacgatgaac gagacggtgg cgtcgctggt
    18001 gggagacgag gccggcatcc tcgccctgaa gcgtttttac ccggagattg cgaagcgtga
    18061 ttacccgttt gtgtacgagc ctccgaagcc ggtcgaggac cccgacgcgc cgccgtccga
    18121 atccgatccg aacgctttca atttcaaccg cgagatgcac gagacgcgcg tgcaggtgga
    18181 cgaatacctg gccacggcgc gcgagctaaa aaggaaagcc ggcgaagcgg cggctgccgg
    18241 tcgggacgag gaagcggcaa ccctgcgtag cgaggcgctc gactggatcg tcagggccga
    18301 aacgtatatg gaggatcgcc gcaagctctt tgtggagaac ggctactggt ggatccgcaa
    18361 gctcaaccag gcttacttcg cgttctacgg ttcgtacgcc gatcgcccgg gcgcgtcagg
    18421 cgaagatccc gtcgggccgg ccgtgcgcga gctgcgcgcg aagataccgc gcatcgccga
    18481 tttcctgaat gccgtcgcgc ccgtcacatc attcgacgaa ctcaagcgcg tgctcgaaca
    18541 gtacccgtag tcaccattgc gtccacaatg cggcgagcag ggcggcgcgc cggacgagac
    18601 tttcgatgac gacgtgctcg tgcagcgcgt gcgcgccgtc ccccagcggg cctagcccgt
    18661 cgagcgtggg aatgcccagc gcggccgtaa agttgccgtc gctgccgccg ccggtcgatc
    18721 cttccttcag ctccagcccg atcgccgcgc cgatgcgccg cgctttctcg aaggtcgcga
    18781 tcatcgtcgc gtcccgctcc atgggcgggc ggttgagacc cccgctcact tcgagccgcg
    18841 cttcgggcaa gacggggcga agcgactgaa acgcctgccg gacccgctcg gcttcggcca
    18901 tcgacgcggc acggaagtcg acgtcgatct cggcgcgatc ggggacgaca ttgctgcgcg
    18961 tgccgcccgc gatcacgccg acgttgatcg ttgtgccgcg ctcgtagtcg gtaagcttct
    19021 gcagcgcaag cacctggtgg gccagctctt cgatcgcgtt gactcctttg tcgtggttgc
    19081 cccccgcgtg cgacgaccgc ccgtgggcga caattttgaa tcccccgacc cctttgcgag
    19141 cggttttcag ctcgccgttc ggcaaggccg gctccatgac cagcacgagg tcgctttgaa
    19201 gcgcctccgc acgaatcaga tcgaccgagg cattgctccc gatctcttcg tcgctggtga
    19261 agagcaggcg aagcggccgg cgcggcgcgc gtcccgagtc gcgcagcgcg ccgatagcct
    19321 gccaggcgat cgcgacgctt cccttcatgt cgtacgcgcc gggaccatac aaccggctgt
    19381 tttcttcccg aatagacatt ctctcgagcg tgcctatcgg ccatacggta tcgaggtgca
    19441 ggagcgcgag cacgcgcccc gaacggccat cgccgcccgg ccagtcggca atcagttgat
    19501 cgccggcggt cgccgacggg atacgctgcg tcgccagccc cagctcgcga aacttcgctt
    19561 ccgtgaaggc cgagcaccga tcgaccgccg ctttgtcggt cgagggcgat tcgagtgcga
    19621 cgagctgttt gagcagcgcg agcgcatcgg ggagtcgccg ggtgcaggcg tcgagcaggt
    19681 cgttcatcgg aattcctttc gcaacttcaa actccaaacc tcaaactcca aagtcccatt
    19741 cttctaaacg cttacaaata ctcagggaat cgagtctggg atcgcatcga cgagctggat
    19801 cagcggctgg gtgacaccgc gcaactggtt gatcaccggg ccgaggccct tctggccgag
    19861 tgggatggcg accggctggt cgaagacgac cgggatttcg ttgctcaccg gcacgaccat
    19921 gctgatgcga atgggtagtg ataggttctg cggcagcgag agagagactg tgccgttgat
    19981 gtgtccaaaa gaccccagcg agaagctggc cgggatggcc agcggcacgg gggcggtggt
    20041 cacggcggtc acattctggt cgaccggcag atcgaagcgc accggcacgc gctggtcgat
    20101 gtgaatggtg tcctggatga cagccgcacc cagcgaggaa aaggccatgt cgagctgcgt
    20161 cagcacgccg ttcagcgtcg ccttaatcgc gccgatctgg ccgatcagga tcaggacgac
    20221 gacgatcagg acgatgttga tcgcgagcga catgacgatc gtgacgttct tcagggcgtt
    20281 ccaggtgctc tgcgaaatgg acacggcaac ctcctcggga ctcgattccg gggtgcaggc
    20341 gcgaatctta tcattaccgc tctgtgagtg caaacggtat atgttgtgcg tagtgcgtcc
    20401 cgttgtaaaa tgtc
//
LOCUS       DBNHXB010000004         3728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1007418, whole genome shotgun sequence.
ACCESSION   DBNHXB010000004 DBNHXB010000000
VERSION     DBNHXB010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1007418"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="AC162G_00165"
     CDS             complement(<1..266)
                     /locus_tag="AC162G_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074259.1"
                     /translation="MGDRSSSTANALANLIMLLGFALLVVGGILAYPSLRESLDLEVA
                     PQGFGNEVALGAAIVQAAESLPQPAPDRPPAQAEIVPPPAVLPET"
     gene            complement(407..1123)
                     /locus_tag="AC162G_00170"
     CDS             complement(407..1123)
                     /locus_tag="AC162G_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074260.1"
                     /translation="MEITSLLPFVSTAVVFVFAVLVFRRYAEKGGKHLLLWGIGLVMY
                     GLGTFAEAALAFAYSPFILHGWYLFGAILTPAWLGQGTVYLLVRRPRVADVLLVILTI
                     ASVFAAIKVFSLPTNDALYDVTLPVSAQYQSVMERDSLTRAMLAIFAAYGTITLVGGA
                     LYSAYIFWRKRIMPQRVLGNVLIAAGALSPALGGVLVTRGLGDYLFASELLGAIIMFI
                     GFRLATTQQPVEAPVAQPAT"
     gene            complement(1222..1377)
                     /locus_tag="AC162G_00175"
     CDS             complement(1222..1377)
                     /locus_tag="AC162G_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074261.1"
                     /translation="MGEPRKTRRQTPRVPSNLLYGKLVPIAIGLMAVILLAVIVAVLL
                     PLFRWGY"
     gene            complement(1370..2011)
                     /locus_tag="AC162G_00180"
     CDS             complement(1370..2011)
                     /locus_tag="AC162G_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024139.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5074262.1"
                     /translation="MADEAGVKSKFGGEWLSLRDAAQVMGVHPATLRSWADQGRIASR
                     RTAGGHRRFSRSELQAWIDARRSHDAGAQVLVQNALGRMRVGLESADAPWLSRFDEAT
                     RRAHRELGRRLLQQLARAASAPGLTDSVRSAADDIGREYAALSLGEVMPLVEAIRAFL
                     FFRDNIVDSIIQMSGALEPIAAPSWLMMHRQICAFLNEVLFALVRAYAENRSG"
     gene            complement(2118..>3728)
                     /gene="priA"
                     /locus_tag="AC162G_00185"
     CDS             complement(2118..>3728)
                     /gene="priA"
                     /locus_tag="AC162G_00185"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR5074263.1"
                     /translation="SGKTEIYMRAVDAMLQRGRQAIVLVPEIALTPQTVRRFAARFPG
                     RVAVLHSGLSEGERYDVWRRARSGAIDILIGPRSAIFAPMARLGLIAIDEEHDGSYKQ
                     DHRPYYHAREVALELARRTGALVILGSATPSLESAARAMRGEFRALALPRRVLGHAQR
                     IRDHEQRFQIASRYEAASGEAFYRELPPVEIVDLRAELKAGNTHMFSRRLQAALKETL
                     DNGEQAILFLNRRGRATFVSCRDCGHVLKCPRCDTPLTYHEPASQRVGESANQLVCHT
                     CNYRQVQPEQCPNCDSRRIRYFGVGTQSVEAAVRELFPDARVLRWDRDTTASRDKTAH
                     EGFLQRFIDGQADVLVGTQMIAKGLDLPLVTLVGVVTADTALNLPDFRSGERTFQLLA
                     QVAGRAGRGLHAGRAIVQTYHPDHYAIVAASQHDYDAFAAQELAFRAEHAYPPATRLA
                     RLIVRDPDASEARRAAETVAASLDAFLARRGSERESIIGPAPCFFSRIAGQSRWHIVV
                     RADDPASLLRDFPLPPNWRLDIDPVNML"
BASE COUNT          721 a         1243 c         1169 g          595 t
ORIGIN      
        1 gtttccggca ggacggccgg gggcggcaca atttcggctt gcgcaggtgg ccggtccggc
       61 gcaggctgcg gcagcgactc ggccgcctgg acgatggccg cgcccagcgc aacctcgttg
      121 ccgaagccct gcggcgcgac ctccaggtcg agcgattccc gaagactggg atacgccagg
      181 atgccgccaa ccaccaaaag cgcgaacccc aaaagcatga tgaggtttgc gagcgcattc
      241 gccgtagaag aagaacggtc tcccatgagc ggagattata cctgtcttcc gtgggcccgc
      301 caatggccct ccggtagtca acgtgcgacg cacttcggaa gtgcgtcgca cgtcagccgt
      361 gcgcgcggat ctcggaagcc gtcacgagag gaaaaatgat gtaaagtcag gtcgccggct
      421 gagcgaccgg ggcctcgacg ggctgctgcg tcgtcgccag gcggaacccg atgaacatga
      481 tgatcgcgcc gagcagctcg ctggcgaaca gataatcgcc caagccgcgc gtgacgagca
      541 caccacccag ggcgggtgag agcgcaccgg cggcgatcag gacgttgccc agcacacgct
      601 gcggcatgat tcgcttgcgc cagaaaatgt acgccgagta cagcgcgccg ccgaccagtg
      661 tgatggtgcc atatgccgcg aagatagcga gcatggcccg cgttagcgag tcgcgctcca
      721 tgacgctctg gtactgcgcg gagacgggta gagtgacatc gtacaacgcg tcgttcgtcg
      781 gcaggctgaa gaccttaatc gccgcgaaca cgctcgcgat cgtcaggatc accagcaaga
      841 cgtcggcgac tctgggccgg cgcacgagta gatacaccgt gccctgcccc agccaggccg
      901 gcgtcagtat cgcgccgaac aggtaccacc cgtgcagaat gaacggcgag tatgcaaagg
      961 ccagggccgc ttcggcgaac gtgcccaggc cgtacatcac caacccgatt ccccatagca
     1021 acaagtgttt gccgcctttt tccgcatagc gacggaacac cagaacggcg aacacgaaca
     1081 cgacggccgt ggatacgaac ggcagcagac ttgtgatttc cattgcaacc tccttaagtt
     1141 ggctccgtga gtcccttgag tttcctgagt tcccattgtt gaggaactca ggaaactgaa
     1201 ggaactcata tcctcacaag gctaataacc ccacctgaat agcggcagca ggacggccac
     1261 aatcaccgcg agcaaaatca cagccatcaa cccgatcgct atcggcacca gtttgccgta
     1321 aaggagattc gacggcacgc gcggcgtttg cctgcgggtc ttgcgcggct cacccactcc
     1381 gattctccgc gtaggccctg acgagcgcga acagcacctc gttgagaaac gcgcagattt
     1441 gccggtgcat catcagccag ctcggcgcgg caatcggttc cagcgcgccg gacatctgga
     1501 tgatcgaatc gacgatgttg tcccggaaga acaggaaagc gcgaatcgct tcgacgagag
     1561 gcatgacctc gcccagtgac aaagccgcat attcgcgccc gatgtcatcc gcggccgacc
     1621 tcaccgaatc ggtcaacccg ggagccgagg cagcgcgagc gagctgctgc agcaggcggc
     1681 ggcccagctc gcgatgagcg cggcgcgtcg cttcgtcgaa gcgagacagc cacggcgcgt
     1741 ctgcgctttc cagccctacg cgcattcgcc ccaatgcgtt ctggaccagc acctgcgccc
     1801 cggcatcgtg cgagcgacgc gcatcgatcc acgcctgtag ctcggagcgg ctgaagcgcc
     1861 gatgtcctcc ggccgtgcgc cggctcgcga tgcggccctg atccgcccag gagcgcagcg
     1921 tcgccggatg cacccccatc acctgcgccg cgtcacgcag ggaaagccat tcgccgccaa
     1981 acttcgattt gacaccggcc tcgtctgcca tgggcaagag gtacgaggaa aatctacaaa
     2041 aaccctcaca cgatggcaca gattgtagcc ggttcgttaa agttcgtcaa ctgactaacg
     2101 tcatcgttcc cgtgattcta caacatgttt accgggtcga tgtcgagtcg ccagttcggg
     2161 ggtagaggaa agtcgcgcag cagtgacgcc ggatcgtcgg ctcgcacgac gatgtgccag
     2221 cgcgattggc cggcgatacg cgagaagaaa cacggcgctg gcccgatgat cgattcgcgc
     2281 tcgcttccgc gccgcgcgag gaaagcatcc aggctggcgg ccaccgtctc cgctgcgcgt
     2341 ctcgcttcac tcgcatccgg gtcgcggacg ataagccgcg ccaggcgcgt cgccggagga
     2401 taggcgtgct cggcccgaaa agcaagctct tgcgcggcga atgcatcgta gtcatgctgt
     2461 gatgcggcga cgatcgcgta atggtcgggg tggtacgtct gcacgatggc gcgcccggcg
     2521 tgcagccccc gcccggcccg gccggcgacc tgcgccaaca actgaaacgt ccgttcgccg
     2581 gatcggaaat cgggcaggtt caacgccgtg tccgccgtga ccacgccgac cagggtgacc
     2641 aacggcagat cgaggccctt ggcgatcatc tgcgtgccga ccagcacgtc tgcctgcccg
     2701 tcgatgaagc gctgcagaaa cccctcgtgc gccgtcttgt cccgcgaagc ggtcgtgtcg
     2761 cgatcccaac gcagcacgcg cgcgtcgggg aacagctcgc gcacggcggc ttccacactt
     2821 tgcgtgccca cgccgaagta gcgtatgcgc cggctatcgc aattcgggca ctgctcgggt
     2881 tgcacttgcc gataattgca ggtgtggcaa acaagctgat tcgctgactc gccgactcgc
     2941 tgactcgctg gttcgtggta agtgagcggc gtatcgcagc gaggacactt cagcacgtgg
     3001 ccgcagtcgc gacaggacac gaacgtcgcc cgcccacgcc ggttgagaaa cagaatcgcc
     3061 tgctcgccgt tgtcgagcgt ctccttcagg gcagcctgca ggcggcggct gaacatgtga
     3121 gtgtttcccg ccttcagctc ggcgcgcagg tcgacgatct cgaccggcgg cagctcgcga
     3181 tagaaggctt cgcccgacgc cgcttcgtaa cgcgacgcga tctggaagcg ctgctcgtgg
     3241 tcgcggatgc gctgcgcgtg gccgaggacg cgccggggga gcgccagcgc gcgaaactcg
     3301 ccgcgcatcg cgcgcgccgc cgattcgagc gagggcgtcg cgctgcccag gatcaccagc
     3361 gcgccggttc gccgcgccag ctccagcgcc acttcgcgtg cgtgataata cgggcggtga
     3421 tcctgcttgt atgacccatc gtgctcttcg tcgatcgcga tcaggccaag ccgagccatc
     3481 ggcgcgaaga tcgccgagcg cgggccgatc aggatgtcga tcgcgcccga gcgcgcgcgc
     3541 cgccacacgt cgtatcgctc gccttcgctc aggcccgaat gcagcaccgc gacccgcccc
     3601 ggaaagcgcg ccgcgaaacg cctcaccgtc tgcggcgtca gcgcgatctc cggcacgagg
     3661 acgatcgcct gcctgccgcg ctgcagcatc gcgtcgaccg cccgcatata aatttcagtc
     3721 ttgcccga
//
LOCUS       DBNHXB010000005         6078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1259, whole genome shotgun sequence.
ACCESSION   DBNHXB010000005 DBNHXB010000000
VERSION     DBNHXB010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1259"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..386
                     /locus_tag="AC162G_00190"
     CDS             75..386
                     /locus_tag="AC162G_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074264.1"
                     /translation="MLIVHSQSGVPVRLSEERWQHIVHRHPELTDQRERVLETVAEPD
                     AIQQGDFGELLAIRLYPETPLTRKFLAVAYRELGSEDGFILTAYLTSRPSARRVTIWK
                     R"
     gene            374..598
                     /locus_tag="AC162G_00195"
     CDS             374..598
                     /locus_tag="AC162G_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017303951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HIR5074265.1"
                     /translation="MEAVRILEKPTAITWDYDEEADVLYLSIGEPQPAVGIDIGEGII
                     LRYDDTHKEVVGLTLIGLRARLLKGLAGGD"
     gene            653..1117
                     /locus_tag="AC162G_00200"
     CDS             653..1117
                     /locus_tag="AC162G_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HIR5074266.1"
                     /translation="MTIQVNSSAFADGSPIPSKYTCDGNDTSPPLKWSGAPQGTKSLA
                     LICDDPDAPAGIWVHWVIYDIPATVTELPEGVPASDVVLGSARQGVNDSRRIGYGGPC
                     PPRGSPHRYYFKLYALDTELTLKPKATKKDVVNAMRGHILAEGQLMGSYKRR"
     gene            1207..1425
                     /gene="xseB"
                     /locus_tag="AC162G_00205"
     CDS             1207..1425
                     /gene="xseB"
                     /locus_tag="AC162G_00205"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011746745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR5074267.1"
                     /translation="MPKKATPAKIEDLTFEQAFEELEATVARLESGDLSLEESLALFE
                     RGQRLSAHCSRLLEQAELKVTQLVTRDE"
     gene            1509..3134
                     /locus_tag="AC162G_00210"
     CDS             1509..3134
                     /locus_tag="AC162G_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR5074268.1"
                     /translation="MELNLRRIPFFAELPDDVLDAIRRRLRVERHPRGAILFREGDLG
                     DTLYLVQSGQLKVYSDATGEERIFAYIGPGGFVGELALLLEQPRSATVAVTIDAEVAL
                     LTKGDLEDLLRDYPAIAIHLGRELGRRLVATSHQPYRREEINLIAVEGPALALAASLM
                     TQAGGRIGLFNLASPIAPERGRGVAIIDPPADMTVDSLAQILGELVGFYERLVVALPY
                     GGGKFHEKIVELADGVAVIGSGKTPAWAQGARRYWSLINEQHEIDRLARLIGRRTVGL
                     VMSSGGAKGIAHVGVLKALKGADVPIDLVAGSSAGSLFGAMFCAGKSIDEIIAFAKEL
                     KKIIQLRGGLWDVAIPPISGLIHGRKTAHFLDKALGGKTFDDLRIPLYIVAADITVGE
                     QVVFNWGPVSDAVRASIGIPGIFAPWPWNGRYLVDGGVVNPVPISVLREHGADRVIVS
                     SVVRTPEERGQVPPDAKRPNFIELMTSMMGAMEGEILKIRLSEVDVFIHPQVHTYSAL
                     DYEKAEELIEIGEMAAREKLPAIQEMLRAQPHY"
     gene            complement(3258..4754)
                     /gene="murJ"
                     /locus_tag="AC162G_00215"
                     /pseudo
     CDS             complement(3258..4754)
                     /gene="murJ"
                     /locus_tag="AC162G_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
     gene            5196..>6078
                     /locus_tag="AC162G_00220"
     CDS             5196..>6078
                     /locus_tag="AC162G_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HIR5074269.1"
                     /translation="MESIILKPTTGPRRLLFVFAHPDDESFGPGGAIARYAREGVEVH
                     YACATRGEVGRVLPEMLTPFEHLPEDQRLGALREGELRCAAMKLGLTGLHLLGYRDSG
                     MPGSADNRDPRAFINADPDRVTGQLVQLIRSIRPQVVVTFDPYGGYGHPDHIFSHQRT
                     TEAFYAAGDPSRYPEAGHPHRPQKLYWSSIPRRMLRIIVNLMPLFGRNPSKFGHNGDI
                     DLRKIVQHDFPITSRIDVRAYLPIKAEAWMCHASQGESSMLFRRFPGVLQRRVLGYER
                     FTRVEPPPNGLRHIEEDL"
BASE COUNT         1215 a         1890 c         1963 g         1010 t
ORIGIN      
        1 aagtgaagtc gggcgacagg ctgaacgtac gggtcataga tggggagttt ggagtgcagg
       61 gggagtgagt cgggatgtta atcgtgcact ctcaaagcgg cgtccctgtt cgcttgagcg
      121 aagagcggtg gcagcatatt gtgcaccgtc atccagaact gacggatcag cgcgaacgcg
      181 ttttggaaac ggtggcggaa cccgatgcga ttcaacaagg tgactttggt gaactacttg
      241 ccatccgact ttatcctgag acgccactga cccgcaaatt ccttgcggtg gcatatcggg
      301 aactcggttc ggaagatggg tttatcctga cggcctatct caccagtcga ccgtcagcca
      361 ggagggtgac gatatggaag cggtaaggat tttggaaaaa ccgacggcaa tcacgtggga
      421 ctacgacgag gaggcagacg ttctctacct gtcgattgga gaacctcagc cggctgtggg
      481 aatcgatatc ggcgagggta ttatcttgcg ctatgatgac acccataaag aagtagtagg
      541 acttaccttg atcgggctac gcgcgcggct gctgaagggc ctggcaggcg gcgactgaaa
      601 gggctcctct tccacgcgag gaccacgctc gtgccagaga agggacatta ccatgaccat
      661 ccaagtcaat agttccgcgt tcgccgatgg atcgcccatt ccgtccaaat acacgtgcga
      721 cggcaatgat acttccccgc cgctgaaatg gagcggcgcc ccgcagggga cgaagagcct
      781 cgcgctgatc tgcgacgacc ccgacgcgcc ggccggcatc tgggtacact gggtgatcta
      841 cgatattccc gcaaccgtca cagaactgcc agaaggcgtg ccggccagcg atgtcgttct
      901 aggcagtgcg aggcagggcg tcaacgattc caggcggatc ggctacggcg gcccgtgccc
      961 gccgcgcggc agcccgcacc gctactattt caagctctac gcgctcgaca cggagctcac
     1021 tctcaagccg aaggcgacaa agaaggacgt cgtcaacgcc atgcggggtc acattctggc
     1081 cgaagggcaa ttgatgggat cgtacaagcg gagatgaagt gagggacttg gaagccagga
     1141 gcacttcaaa ctccaaagcc caaactccag actctaactc taactctctc acttcgagag
     1201 gccatcatgc cgaagaaagc gacccctgcc aaaatcgagg atctgacatt cgagcaagcc
     1261 ttcgaggagc ttgaggcgac cgtggcgagg ctcgagagcg gcgatctgtc gctcgaagag
     1321 tcgctggcgc tgttcgagcg cggccagcgc ctgagcgcgc attgcagccg cctgctggag
     1381 caggccgagc tgaaggtgac gcaactcgtg acacgtgatg agtgaaacgt gacgggagcc
     1441 tcgtcacgca tcacgtttca cgtttcacgc atcacgtttt acgtttcacg catcacgctt
     1501 cacgctgaat ggaactcaac cttcgacgca tcccgttttt cgccgagctg cctgacgatg
     1561 tgttggacgc gatcaggcgc cgcctgcgcg tcgagcgcca cccgcgcggc gcaatcctgt
     1621 tccgcgaggg cgacctggga gatacgctgt atctcgtcca gtcgggccag ttgaaggtgt
     1681 attctgacgc gaccggcgaa gagcgcattt tcgcctacat cggcccgggc ggcttcgtcg
     1741 gcgagctggc gctgctgctc gagcagccgc gctcggcgac cgtcgcggtc acgatcgacg
     1801 ccgaagtggc gctgctgacc aagggcgacc tggaggatct cctgcgcgac tacccggcga
     1861 tcgcgatcca cctcgggcgc gagctgggga ggcggctggt cgccacgtcg caccagccct
     1921 accggcgcga ggagatcaac ctcattgcgg tcgaaggccc agcgctcgcc ctggcggcca
     1981 gcctgatgac acaggcggga ggccgcatcg ggcttttcaa cctggcgtcg ccgatcgcgc
     2041 cggagcgcgg gcggggtgtc gcgatcatcg atccgcctgc cgacatgacc gtggacagcc
     2101 tggcgcagat tctgggcgag ctggtcggat tctacgagcg cctggtcgtc gcgctgccgt
     2161 acggcggcgg caagttccac gagaagatcg tcgagctggc cgacggggtt gccgtgatcg
     2221 gctcgggcaa gacgccggcc tgggcgcagg gcgcacgccg ctactggtcc ctgatcaacg
     2281 agcagcacga gatcgatcgc ctggcgcgtc tcatcgggcg gcgcacggtg gggttggtga
     2341 tgtcgagcgg cggtgcgaag ggcatcgcgc acgtcggcgt gctcaaggcg ctgaaaggcg
     2401 ccgacgtgcc gatcgacctc gtggccggat cgagcgccgg atcgctgttc ggggcaatgt
     2461 tctgcgccgg caagtcgatc gacgagatca tcgccttcgc caaagagctg aagaagatca
     2521 tccaactgcg cggcgggctg tgggacgtcg cgatcccgcc gatctccggc ttgatccacg
     2581 gtcgcaagac ggcgcacttc ctcgacaaag cgctgggcgg caagaccttc gacgacctgc
     2641 gcatcccgct ctacatcgtc gccgccgaca tcacggtcgg cgagcaggtg gtgttcaact
     2701 ggggaccggt ctccgacgcg gtgcgcgcgt cgatcggcat tcccggcatc ttcgcgccgt
     2761 ggccatggaa tggacgctac ttggttgacg gaggcgtcgt caaccccgtg ccgatcagcg
     2821 tgctgcggga gcacggcgcg gatcgcgtca tcgtttcgag cgtcgtgcgc acgcccgaag
     2881 agcgcggcca ggtgccgccg gacgccaagc ggcccaattt catcgagctg atgacgtcga
     2941 tgatgggcgc gatggaaggc gagatcctca agatacgcct atcggaagtg gacgtcttca
     3001 tccatcccca ggtgcacacg tacagcgcgc tcgattacga gaaggccgaa gagctgatcg
     3061 agatcggcga gatggctgcg cgcgagaaac tgccggccat tcaggagatg ctgagggcgc
     3121 agccccatta ttaagaacct atccgaaaac gcaggtggga cggataacgt aggcgggcat
     3181 ccccatgctc gcccagacgc gcggcacgtg tcgcgcagcg cctgcccgcg cccctgaacg
     3241 agctgccgca cgctgcgcag ctcgtcggcg cgcagcagga atgccgctcc ggcatacacg
     3301 atcaccccca ctgcgatccc ggccgcgcac agcgccaatg tgctgctcat cggcatttgc
     3361 agcagcccca gcagcgccag tcccatcgcg gccgaggcca ggccggatcg agcgagcgcg
     3421 ccccacaccc tgcgaccttc gatgccgctc agccgcccgc ggatcaacca gaccagggcg
     3481 agcgcttcaa ccgtcgtcgc gatcgagttt gccagcgcca ggccgccctc cgccagcggg
     3541 ccgatcagca gcagcgacag cgccacgttc aaaagcattg ccgccacgcc ggcgatcacc
     3601 ggcgtgcgtg tatcgtgcat cgcgtagaag ccgcgcgtga cgatctccag cagcgaatgt
     3661 gcgaccagtc ccagcgcgaa gaactgcagc gtataggcga cggcttgcgt ggactcaggc
     3721 gtgaactcgc cgcgctcgaa caacaactgc accagcggcg tgcgcaggac gatcaggccg
     3781 acggcggcgg gcaggctgag aaacagtgtc gcccgcagcg cgccggacag cgtgtgacgc
     3841 atcgggtcga tttcgttccg ggcggcctgc gcggcgaagg tcgggaaggc ggcggtggcg
     3901 atcgactgcg cgaacacgcc cagcggcagg agcatgagaa gccaggcata attcagcgcc
     3961 gacacggcgc cctcgcccag gcgcgaagcg aggttcgtgt tgatcaggaa gttaagctgg
     4021 acggcggcca ggcccagcac gcgcggtccc atgagccgcg caacgtgtac cacgccggga
     4081 tcgcgcacgt cgaagacggg aaagtagcgc gcaccgaacc tcaccaggcc gggcgcctga
     4141 atcaggagat gccccgccgc gccggcgacg acgccaatgg ccagaccggc cacgccgacg
     4201 aacggtgaca ggaatgccgc tcccgcgatg atcgcaaggt tgtacgcgat cggcgcaagc
     4261 gcaggcaaga ggaaatgctg ttgcgcgttg agcgcgccca tgatcacacc gctcgcgccg
     4321 aagatgacgg gggcgatcag catcacccgc atcagggata cggccagcgc ctgcgtggcg
     4381 gggtcgaacc cgggcgcgat gaccgtggct accaatgccg gcgcgaatat cgcggcgagc
     4441 gcagccgccg tggagagaat cagcgtgatg atgttgacga cggcgctggt gagtcgccac
     4501 gcgcccagac ggtcgccggc ggtgaggtaa cgcgtgaagg tgggaataaa ggcggatgcc
     4561 agcgcgcccc ctgctatcag catgtagagc agatcgggca gccggaaagc agccaggtag
     4621 gcgtcgtact cgccggaagt gccgaaccgc gcgccgatca ccatctcccg caccaggccc
     4681 aggatgcgtg aggcaataaa agccagcatc acaatgctgg cggcgcgagc gatttggcgc
     4741 gcaggggagg tcatggcgag gattatacca ctcaggtgcc cggcaaaata aagccaaaag
     4801 agtcaaaggt cgctgccctt tgactctcgg ctaaaggagg agaagaagag gtttccgccc
     4861 gctactcttc gagagtcaat atacaccgaa ccggcctgga gggcatgtcg cccgccctac
     4921 gctttgccta cgctggcgtt aaccattcct tacaaatgcc atggttttgc aatgctttgg
     4981 cgtccaggct gaacggattg ccagagacag cacaaactcc gatccgctct cggcggattg
     5041 acaaatccgc cgctggattt ggcaatcgct tatccggcgg cgtgggagcc gcctccgctg
     5101 acatgttttg cgtacacact cccatttctg gaactgacag tctgaaagga ttgtgatatg
     5161 atcagtctcg gatcaagcaa aggcgctccc ggattatgga gtccatcatt ctgaagccga
     5221 cgacaggccc caggcggctg ctgttcgtct ttgcacatcc tgacgacgaa tccttcgggc
     5281 ctggcggcgc catcgcgcga tacgcccgtg agggtgtcga agtgcattat gcctgcgcga
     5341 cgcgtggcga agtcggccgg gtcctgccgg agatgctcac gccgttcgag cacttgccgg
     5401 aggatcagcg gctcggcgcg ctgcgcgagg gcgagctgcg ttgtgcggcg atgaagctgg
     5461 gcctcaccgg gctgcacctg ctgggctatc gcgactccgg catgcctggc agcgccgaca
     5521 atcgcgatcc gcgcgccttc atcaacgccg atcctgatcg agtaaccgga caactggttc
     5581 agttgatacg ctcgatccgg ccgcaggtcg tcgtcacatt cgatccgtac ggcggctacg
     5641 gtcaccccga tcacatcttc tctcaccagc gcacgacaga ggcattctat gccgccggcg
     5701 atccgagtcg ctacccagag gccggccacc cgcaccgtcc gcagaaactg tactggtcga
     5761 gcattcccag gcgaatgctc aggatcatcg tgaacttgat gcccctgttc gggcgcaacc
     5821 cgtcgaaatt cggccacaac ggcgatatcg acctccgaaa gatcgtacag cacgatttcc
     5881 cgatcacgtc caggatcgac gtccgggcgt atctaccgat caaagctgaa gcgtggatgt
     5941 gccatgccag ccagggcgag tcgagtatgc tctttcgaag attccccggc gtgttgcaac
     6001 gccgggtact gggatacgag cggttcacac gcgtggaacc gccgccgaac gggcttcgtc
     6061 atatcgaaga ggacttgt
//
LOCUS       DBNHXB010000006         4209 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_169182, whole genome shotgun sequence.
ACCESSION   DBNHXB010000006 DBNHXB010000000
VERSION     DBNHXB010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_169182"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /gene="atpA"
                     /locus_tag="AC162G_00225"
     CDS             <1..1324
                     /gene="atpA"
                     /locus_tag="AC162G_00225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR5074270.1"
                     /translation="QLGVIILGEYAGIEEGQLVRRTGRIISVPVGGTLLGRVVDPLGR
                     PVDGKGSIATTRYRNVERIAPGVIERQNVDTPVETGIKPIDAMIPIGRGQRELIIGDR
                     QTGKTAICIDAIINQKGKSMVCIYVAIGQKEAQVARTVATLEKYGAMDYTIVVDASAS
                     DPAALQYIAPYAGCAMGEEVMEEGVTVGGHLIKDALVVYDDLSKHAWAYRQVSLLMRR
                     PPAREAYPGDVFYLHSRLLERAARLSQARGGGSLTALPIIETLLGDVSAYIPTNVISI
                     TDGQIYLETDLFNAGIRPAINVGISVSRVGGDAQIRAMRQVAGRLKGDMAQFRELAAF
                     AQFGSDLDAATRRQLDRGQRLTEILKQVQYAPMPIENQIMVIFAGTNGFLDQVPVDRA
                     REWENAFHKFMDTQHPQIGEEIAREKKMSDALIAKLRSACEEFSKTWS"
     gene            1496..2428
                     /gene="atpG"
                     /locus_tag="AC162G_00230"
     CDS             1496..2428
                     /gene="atpG"
                     /locus_tag="AC162G_00230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR5074271.1"
                     /translation="MATIREIRRRIRSVKNIAQVTRALEAVSASKVRKAQAQVLATRP
                     YAQQAWEVLTSLALQAGAGASIHPLLAARPVRSVGLIAITGDRGLAGAYNFNVVRMTM
                     EFIRAQTAPIQIVAVGRKGRELLIRARQNVVAEFSNLSAAPTALEIGPIGRIVVDDFL
                     AEKIDEVYVAYTDFISTLQQKPAIKRLLPLSIEEGERVMAEYTAGAQAGRLTRRDYIY
                     EPGREELLDVVIPRFVGLQVYQAVLESLASEHSARMVAMRNATDSALGLVGELTLTYN
                     KARQQAITSELLDIAGGAEALKQALARTPVPSVP"
     gene            2481..3896
                     /gene="atpD"
                     /locus_tag="AC162G_00235"
     CDS             2481..3896
                     /gene="atpD"
                     /locus_tag="AC162G_00235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR5074272.1"
                     /translation="MATNRMTGKVVQVQGGVVDVEFPEGDLPEIYYALEIPRDGSQTL
                     ILEVQQQLGNSRVRTVAMGTTDGLSRGAPVRNTRQPISVPVGQVTLGRIFNVLGQPVD
                     GKGPVESQVKYAIHRKAPPFEEQSTRTEVFETGIKVIDLIAPFTKGGKTGVFGGAGVG
                     KTVIIMELIRSIAAEHGGYSVFAGVGERTREGTQLYREMIESGVIDRTVMVFGQMNEP
                     AGVRLRVGLTGLTMAEYFRDEGRDVLLFIDNIFRFSMSGSEVSALLGRLPSAVGYQPT
                     LGNEMGELQERITSTKRGSITSMQAVYVPADDYSDPAPVATFAHLDATIALERAIAEK
                     GIFPAVDPLASTSRILDPNIVGPDHYTTAREVQRVLQRYKDLQDIIAILGVEELSEDD
                     KLLVARARKIERFFSQPFYVAQQFTGRDGRYVPLRETVRGFREILDGKHDDVPEQAFY
                     MCGPIDDVVANAENLRRGESI"
     gene            3956..>4209
                     /gene="atpC"
                     /locus_tag="AC162G_00240"
     CDS             3956..>4209
                     /gene="atpC"
                     /locus_tag="AC162G_00240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR5074273.1"
                     /translation="MPTHLEIVTMERRVYSEDVDMVIAPGSEGELGILPNHTPLLTHL
                     KEGVLRVRRGGQEEAFAIGGGIMEVLPNKVIVMADAAEHS"
BASE COUNT          810 a         1309 c         1382 g          708 t
ORIGIN      
        1 tcagcttggc gtcatcatct taggcgaata tgcaggcatc gaagaaggcc agttggtgcg
       61 ccgcaccggg cgcatcatct ccgtgccggt cggcggcacg ctgcttggcc gcgtggtcga
      121 tccgctggga cgcccggtcg acggcaaagg ctcgatcgct acgaccaggt atcgcaacgt
      181 cgagcgcatc gcgccaggcg tgatcgagcg ccagaacgtc gatacgccgg tcgagacggg
      241 catcaagccc atcgacgcga tgattccgat cggccgcggc cagcgcgagc tgatcatcgg
      301 cgaccggcag acgggcaaga cggcgatttg catcgacgcc attatcaatc agaagggcaa
      361 gagcatggta tgcatctacg tcgccattgg ccagaaagag gcccaggtgg cgcgcacggt
      421 agccacgctc gagaaatacg gcgcgatgga ttacacgatc gtcgtcgacg cctcggcctc
      481 cgatccggcc gcgctgcagt acatcgcccc ctatgcgggc tgcgcgatgg gtgaagaagt
      541 gatggaagag ggcgtcacgg tcggcggcca tttgatcaaa gatgccctgg tcgtctacga
      601 cgacctgagc aagcacgcct gggcgtatcg ccaggtgtcg ctgctcatgc gccgcccgcc
      661 cgcgcgcgag gcgtatccgg gcgacgtgtt ctatctccac tcgcggttgc tcgagcgcgc
      721 ggcccggctg agccaggccc gcggcggcgg ctcgttgacg gcgctgccca tcatcgagac
      781 gctgctcggc gacgtgtcgg cgtatattcc caccaacgtg atctcgatca ccgatggcca
      841 gatatatctc gaaacggatc tgttcaacgc cggcattcgc ccggcgatca acgtcggcat
      901 ctccgtgtcg cgcgtcggag gcgacgcgca gattcgggcg atgcgccagg tcgccggccg
      961 ccttaaaggc gacatggccc agttccgcga gttggctgca tttgcccaat tcggctccga
     1021 tctggacgct gctacccgcc ggcaactgga tcgcggacag cgcctgaccg agatcctcaa
     1081 gcaggtgcag tacgcgccga tgcctatcga aaatcagatc atggtcatct tcgcgggcac
     1141 gaacggcttc ctcgatcagg ttccggtgga tcgcgccagg gaatgggaga acgcgttcca
     1201 caagttcatg gatacgcagc accctcaaat cggcgaagag atcgcgcgcg agaagaagat
     1261 gagcgacgcg ctgatcgcga agctgcgctc ggcgtgcgaa gagttcagca agacgtggtc
     1321 gtgagcgtaa atcgtgaagc gtgaagcgtg atgcgtggtg cgtggtgcgt gaagcgtgaa
     1381 ccgggagtcg ccgctcatgc gatgggtaac aagtgatagg cgacgagcga taggcgacag
     1441 acgatacgcg atatgcgata ggcgatacgc gatacgcgat acgcaatagg cgaccatggc
     1501 aacgattcga gagattcgac ggcgtatccg cagcgtcaag aacatcgccc aggtgacgcg
     1561 ggcgctcgaa gccgtctcgg cgtcgaaggt gcgcaaagca caggcgcaag tgctggcgac
     1621 gcggccgtac gcgcagcagg cctgggaagt gctgacctcc ctggcgctgc aggcgggggc
     1681 cggagcgagc atccatcccc tcctcgccgc gcgtcccgtg cgatccgtcg gcctgatcgc
     1741 catcaccggc gatcgcggtc tggccggcgc gtacaacttc aatgtcgtgc gcatgacgat
     1801 ggagttcatc cgcgcccaga cggcgccgat ccagattgtc gccgtcggcc gcaagggtcg
     1861 cgagctgttg atccgcgcgc gccagaatgt cgtcgcggag ttttccaatc tgtccgccgc
     1921 gccgacggcg ctggagatcg ggccgatcgg ccgcattgtc gtcgacgatt ttctcgccga
     1981 gaagatcgac gaggtctacg tagcctacac agacttcatc agcacgctgc agcagaagcc
     2041 ggccatcaag cgcctgctcc cgttgtcgat cgaagagggc gagcgcgtga tggccgaata
     2101 cacagccggc gcgcaagcgg ggcgcctgac ccggcgcgac tacatctacg agcctggccg
     2161 cgaggaactg ctcgacgtcg tgattccgcg cttcgtcggc ttgcaggtct atcaggccgt
     2221 gctcgagtcg ctggccagcg agcattcggc gcgcatggtc gccatgcgca acgccacgga
     2281 cagcgcgctc ggcctcgtcg gcgagctgac gctgacctac aacaaggcac gccagcaggc
     2341 gatcacgagc gagctgctcg acatcgccgg cggcgccgaa gcgctcaagc aggcgttggc
     2401 tcgtacacca gttccttcag ttccctgagt tcccgcaagg agactcaagg aactcaagga
     2461 actcagggga ggataacttg atggcaacga atagaatgac gggcaaggtc gtgcaagtgc
     2521 aaggcggcgt ggtcgacgtc gaattcccgg aaggcgatct gcctgagata tattacgcgc
     2581 tcgagattcc ccgagacggg agccagaccc tgattctgga agtgcagcag cagctcggca
     2641 acagccgcgt gcgaacggtc gccatgggca ccacggatgg cctgtcgcgc ggcgccccgg
     2701 tgcgcaacac ccggcaaccc atcagcgtcc cggtcggcca ggtgacgctg ggccgcatct
     2761 tcaacgtgct gggacagcct gtcgacggaa aaggcccggt cgagagtcag gtgaagtatg
     2821 cgattcaccg caaggcgccg ccgttcgagg agcagtcgac gcgcaccgaa gtgttcgaga
     2881 ccggcatcaa agtcatcgac ctgatcgcgc cgttcaccaa aggcggcaag accggcgtgt
     2941 ttggcggcgc gggcgttggc aagaccgtca ttatcatgga gctgatccga tcgatcgccg
     3001 ccgagcacgg cggctattcc gtgtttgccg gcgtcggcga gcgcacgcgc gaggggacac
     3061 agctttacag agaaatgatc gaatcgggcg tcatcgacag gacggtaatg gtgttcggcc
     3121 agatgaacga gccggccggc gtgcgcctgc gtgttgggct gaccgggctg acgatggccg
     3181 agtacttccg cgacgagggc cgcgacgtcc tgctgttcat cgacaatatc ttccgcttct
     3241 cgatgtccgg ctcggaagtg tccgccctgt tgggacgctt gcccagcgcc gtcggctatc
     3301 agccgacgct cggcaacgaa atgggcgagc tgcaggagcg catcacttcg accaagcgtg
     3361 ggtcgatcac ttcgatgcag gcggtctacg ttccggccga cgactactcc gaccccgctc
     3421 cggtggcgac atttgcccac ctcgacgcga cgatcgcgct cgagcgcgcc atcgccgaaa
     3481 aaggcatttt cccggccgtc gatccgctgg cttcgacgtc gcgcatcctc gatccgaaca
     3541 tcgtcggccc cgatcattac accacggcgc gcgaggtgca gcgcgtgctc cagcgctaca
     3601 aagatctgca ggacatcatc gccatcctcg gcgttgaaga gctgagtgag gatgacaagc
     3661 tgctcgtggc gcgtgcgcgc aagatcgagc gctttttctc ccagccgttc tacgtcgcgc
     3721 agcagttcac cggacgcgac ggtcgctatg tgccgctgcg cgagacggtg cgtggcttcc
     3781 gagagatcct ggacggcaag cacgacgacg ttcctgagca ggcgttctac atgtgcgggc
     3841 cgatcgacga cgtcgtcgcc aatgccgaga acctcaggcg cggcgagagc atctagcgcg
     3901 cgacgccgtc cgcgtgcgcg cgaatacgca acgctgaaag tcgactggag ccgatatgcc
     3961 cactcacctt gagatcgtca ccatggaacg ccgcgtctac tccgaggacg tcgatatggt
     4021 catcgcgccg ggttcggagg gcgagcttgg catcctgccg aaccacacgc cgttgctcac
     4081 ccacctgaag gaaggggttt tgcgcgtcag gcgaggcggc caggaggagg ctttcgcgat
     4141 tggcggcggg atcatggagg tgcttcccaa caaggtcatt gtgatggccg atgcggccga
     4201 gcattccga
//
LOCUS       DBNHXB010000007         3509 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1679032, whole genome shotgun sequence.
ACCESSION   DBNHXB010000007 DBNHXB010000000
VERSION     DBNHXB010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1679032"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..978)
                     /locus_tag="AC162G_00245"
     CDS             complement(28..978)
                     /locus_tag="AC162G_00245"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR5074274.1"
                     /translation="MTAVGIDVAKKTLAVDAMLPTGKRRQKACANTAAGHAELVRWLG
                     KHAVAPVVIGLEATGGYQEAVALALHDAGYAVSVLNPSVIAAYGQSQLRRAKTDPTDA
                     ALIADYMRTQQPPAWTPLPRETRHLQALARRLEALLEMRTQEANRLDLAAAVVRPSID
                     ATIAHLDAQIAGVKRQMADHIDRHPTLRQQRDLVQSIPGIGATTAAIILGELDVKRFA
                     SARQLAAYTGLVPRVRQSGTSVRGRGVLVKIGAGRIRKALYFPALTAVRCNPSLVRFA
                     ARLRAAGKASLVIVAAVMRKLVHQIYAVLSSGRPYDPAKA"
     gene            1414..1842
                     /locus_tag="AC162G_00250"
     CDS             1414..1842
                     /locus_tag="AC162G_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017941949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR5074275.1"
                     /translation="MAFIEDPLVHEVIGCAIAVHRELGPGLLESAYNRCFRLELVHRK
                     VRFSNEVWLPILYRGTSLDNAYRMDFMVEDWLIVEVKAIEKVLPVHRAQLLTYMRLSR
                     ARQGLLFNFNEPRLKDGIHSVILGGSASSPSTTPSTVENQ"
     gene            complement(1894..>3509)
                     /locus_tag="AC162G_00255"
     CDS             complement(1894..>3509)
                     /locus_tag="AC162G_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR5074276.1"
                     /translation="RKLGFLVVQGYGLTETAPIVTLNHPLRASKGTVGRPMHGVDVKI
                     APDGEILVKGDNVTTGYYGHQSDSAFDANGYFHTGDIGEIDPDGRLLIKGRKKEMIVT
                     PQGLNVFPEDVERALLAQPGVKDAAVVGRNVDGEERVHAVLVLESGADERAIVRGANA
                     VLEDHQRVRSTSVWPGESLPRTDGTQKLKRREIQRWAAGETAGVGVTSAAGTSVESVV
                     ARFASGQDITPGTTMEELGLSSLDRVELMMALEEAFQTTLDESVLTTARTVGELKALL
                     GSGPQVPGSGATAAEPEPGTRNLEPGTRNQEPEPSAEPRWNRTSLSWLLRRISLPTWI
                     LPLARYFLRPLTVHGLEHLAALKGPAIFASNHQSHMDTPVIMLAMPAKWRYSVAPAMA
                     KEFFQAHFFPARFTRKEWFTNSLNYYLSCQFFNAFPLPQREAGTRRTMRYMGEILADG
                     YSILIFPEGKRTQDAIAPFRPGIGMMGSKLGVPVVPVRIEGVDKVLHPKWKWPAKGPV
                     VVKFGKPLTLAGDDYAALATQVEEAVKGL"
BASE COUNT          631 a         1148 c         1067 g          663 t
ORIGIN      
        1 atcaacatga agataccgtg ttgactgtca agccttcgcg ggatcgtacg gccggccgga
       61 gctgagaacg gcgtagatct gatggacgag cttgcgcatc acggccgcca cgatcaccag
      121 ggaggctttg ccggcggcgc ggagacgggc cgcgaagcgc acgaggctcg gattgcagcg
      181 aaccgcggtg agcgcgggaa agtacagggc tttgcggatc cgtccggcgc cgatcttcac
      241 gagcacgccg cgaccgcgga ccgacgtgcc agactgccgg acgcgtggca ccaggccggt
      301 gtaggccgcc agttgccgcg cactggcgaa gcgtttgacg tcgagctcgc cgaggatgat
      361 cgccgccgtc gtcgcaccaa tcccgggaat cgattgcacg agatcccgct gctggcgcaa
      421 cgtcggatgg cgatcgatgt gatcggccat ctggcgcttg acgcccgcga tctgcgcgtc
      481 cagatgcgcg atcgtcgcgt cgatcgacgg ccgcacgacc gcggcggcca gatccagacg
      541 attggcttcc tgcgtccgca tctcgaggag cgcctcgagc cgccgcgcca gcgcctgtaa
      601 gtgccgcgtt tcccgcggta acggggtcca ggccggcggc tgctgcgtgc gcatgtagtc
      661 ggcaatcagg gccgcatcgg tcggatccgt tttcgcgcgc cgcagctggc tctggccata
      721 cgcggcaatg acactggggt tgagcacact gacggcatag cccgcgtcgt gcaacgcgag
      781 cgccaccgcc tcctgatacc cgccggtcgc ttccagcccg atcaccacag gcgccacggc
      841 gtgcttgccc agccagcgca cgagctccgc gtgccccgcc gccgtattcg cacaggcttt
      901 ctgccgccgc ttgcccgtcg gcaacatcgc gtccaccgcg agcgttttct tcgccacatc
      961 aatcccgacc gctgtcattg tcgtgatcct ttctgtggcc gccggagaca gtcacccttg
     1021 cacatgcagg ctcagccctg acgaggcggc cttcgatacc gttcgacctg gcgccacaca
     1081 gcgagcgccg cgcgctgctc ttcatgacag gctcgaagcc tcaggcatga accagcgtca
     1141 cggcgctcct actcaagcgt ccccgagtag actgcgtcac cgcagcgtcg tcaacataca
     1201 aggcgtgaag gacatgaaga gagctgaaga aaacctacgg attctcgacg gtcgaagaaa
     1261 ggctgaaggc ctcagaaaga acccacttca agaaggacat tgaggaaatt gccaactggc
     1321 tcgataccga cttcgcagaa aacgcgatcg cgctttcgac atccggcgac tgatgcgcaa
     1381 cgcgcaggga tcgaatcttg ctcttaggtg agcatggctt tcatcgaaga cccgctggtt
     1441 catgaggtta taggctgtgc cattgcagtt catcgagaac ttggaccagg gcttctcgag
     1501 tcggcgtaca accggtgttt caggctggag ctggtgcacc gcaaggtccg gttctccaat
     1561 gaggtttggt tgccaatcct gtatcgagga acctcgctcg acaatgcgta ccggatggat
     1621 ttcatggttg aggactggtt gattgtcgaa gtcaaggcga ttgaaaaggt ccttccggtg
     1681 cacagagcgc aactcttgac ctacatgcgt ctctcgcgcg cgcggcaggg actgctgttc
     1741 aacttcaacg agccgcgcct gaaggacgga atccacagcg tgatccttgg agggtcggcg
     1801 tcttcaccgt ctacaacccc ttcgaccgtc gaaaatcagt aggttttctt cagcactctt
     1861 cacgtccttc acgccttcat gttgataggt cttctacaat cccttcaccg cctcctcgac
     1921 ctgcgtcgcg agcgcggcgt agtcgtcacc ggcgagcgtc agcggcttcc cgaacttcac
     1981 gaccaccggc cccttggccg gccacttcca cttcgggtgc agcaccttgt ccactccctc
     2041 aatgcggaca gggacgacgg gtaccccgag cttcgatccc atcatgccga tgccggggcg
     2101 gaagggcgcg atggcgtcct gggtgcgctt gccttcggga aagatcagga tcgagtagcc
     2161 gtccgccaga atctcgccca tgtaccgcat ggtgcggcgc gtgccggctt cgcgctgcgg
     2221 cagcggaaac gcgttgaaga actgacacga taagtaatag ttcaggctgt tcgtgaacca
     2281 ctccttgcgc gtaaagcgcg cggggaagaa gtgcgcctgg aagaactcct tggccatggc
     2341 cggcgccacg ctgtagcgcc acttcgccgg catcgccagc atgatcaccg gcgtatccat
     2401 gtggctctga tgatttgacg caaagatcgc gggccccttc agcgcggcca gatgctccag
     2461 tccgtgcacc gtcagcggac gcagaaagta tcgggcgagc ggcagaatcc acgtcggaag
     2521 gctgatgcgg cgaaggagcc aggaaaggga agtgcggttc cagcgaggtt cagctgatgg
     2581 ttcgggttcc tggttcctcg ttccaggttc gaggttccgg gttcctggtt ctggttcagc
     2641 cgccgtagca cccgaacctg gaacctgggg cccggaaccc agcagcgctt tcaactcccc
     2701 aacagtccgt gccgttgtga ggaccgactc gtcgagcgtc gtctgaaacg cttcttcgag
     2761 cgccatcatc agctcaacgc gatccagcga gctcagtccc agctcctcca tcgtcgtgcc
     2821 gggcgtgatg tcctggccgc tcgcaaagcg cgccaccacg gactccacac tggtgcccgc
     2881 tgcggacgtg actccgacgc ccgcggtttc gcccgcggcc cagcgctgaa tttcgcgccg
     2941 cttcagcttc tgtgtgccgt ctgtgcgggg aagcgattcg cccggccaga cggacgtgct
     3001 ccgcacccgc tgatggtcct cgagcacggc gttcgcccct cgcacgatcg cgcgttcgtc
     3061 ggcgcctgac tccaggacca acacggcgtg cactcgctct tcaccgtcaa cgttccggcc
     3121 gaccaccgcg gcatccttca cgcctggttg cgcaagcagc gcccgctcga catcctcagg
     3181 aaacacgttc agtccctgcg gcgtgacgat catttccttc ttgcggccct tgatcagcag
     3241 ccgcccgtca ggatcgatct ccccgatgtc gcccgtgtgg aagtatccgt ttgcgtcgaa
     3301 agcgctgtcc gattgatggc cgtagtaacc ggtggtgacg ttgtcaccct tgaccaggat
     3361 ctcgccgtcg ggcgcgatct tgacgtccac gccgtgcatc ggacgtccga ccgtgccctt
     3421 cgaggctcgc aacgggtgat tgagcgtaac gatgggggcg gtttccgtga gtccgtagcc
     3481 ctgcacgacg agaaagccga gcttccgcc
//
LOCUS       DBNHXB010000008         2110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3524427, whole genome shotgun sequence.
ACCESSION   DBNHXB010000008 DBNHXB010000000
VERSION     DBNHXB010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3524427"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="AC162G_00260"
     CDS             <1..729
                     /locus_tag="AC162G_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5074277.1"
                     /translation="MRHVRGRQIAMIFQDPMTSLNPVMPIGRQIGEMLELHIGMTHRQ
                     ARDRAAELLTMVGIPRAADRLDDYPHQFSGGMRQRVMIAMALSCAPQMLIADEPTTAL
                     DVTIQAQIVDLVRQLRGELGMAIVWITHDLGVVARLVQKVMVMYAGYVVERAPVSDLY
                     ERPGHPYTLGLLGSLPRLDDLERRRLVSIEGSPPDLVDLPKGCPFAPRCRFVIGRCRE
                     ENPPLLPVADGHEAACWVAATTWL"
     gene            768..1787
                     /locus_tag="AC162G_00265"
     CDS             768..1787
                     /locus_tag="AC162G_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009785036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5074278.1"
                     /translation="MRERSGDRSRTGNSSDSNSMLTVQNLKKYYPIRRGVFRQHAGDI
                     KAVDGVSFTIRRGETLGLVGESGCGKSTTGRSILQLERPTAGEVIFEGRELTKLRGEE
                     LRRMRRHMQIIFQDPYASLNPRMTVGEILNEPLQAHGAAASGARRERVRELLSLVGLD
                     PSFAARFPHEFSGGQRQRIGIARALALNPLFVVCDEPIAALDVSIQAQIVNLLKDLQA
                     RLGLTYLFIAHDLSMVRHISDRVAVMYLGRLVEIADWGDVYADPKHPYTQALLSAIPI
                     PDPKVEARRQRIVLEGDVPSAASPPKGCHFNTRCPIRQERCYHEDPELREIAPGHWAA
                     CHFAA"
     gene            1907..>2110
                     /locus_tag="AC162G_00270"
     CDS             1907..>2110
                     /locus_tag="AC162G_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HIR5074279.1"
                     /translation="MNRKFAMTGILILAVLASACAPASTPTAPPATQAPGAAEPPQVE
                     PTKPAEPTAAPAAPEPKDKETFVQ"
BASE COUNT          370 a          673 c          714 g          353 t
ORIGIN      
        1 atgcggcacg tgcgcggcag acagattgcg atgatcttcc aggacccgat gacgagcctc
       61 aaccccgtca tgccgatcgg ccggcagatc ggcgagatgc tggagctgca cataggcatg
      121 acccaccgac aggcgcgcga tcgggctgcc gagctgctga cgatggtcgg catcccgcgc
      181 gccgccgatc gactggacga ctacccacat cagttctccg gcgggatgcg ccagcgcgtg
      241 atgatcgcga tggccctgtc gtgcgcgccg cagatgctga tcgccgacga gccgacgacc
      301 gcgctggacg tgacgatcca ggcgcagatc gtcgacctgg tgaggcagct gcgcggcgag
      361 ctgggcatgg ccatcgtctg gatcacgcac gacctgggtg tggtcgcccg gctggttcag
      421 aaggtgatgg tcatgtacgc cggctacgtg gtcgagcgcg caccggtgag cgatctgtac
      481 gagcggcccg gccaccccta cacgttgggg ttgttaggct cgctaccgcg cctggacgat
      541 ttggagcggc gtcgcctggt gtcgatcgaa ggctcgccgc ctgacctggt cgatctgccg
      601 aagggctgcc cgttcgctcc gcgctgccgt ttcgtgatcg ggcgctgccg tgaagaaaac
      661 ccgccgctcc tgcccgtggc cgacgggcac gaggccgcgt gttgggtagc ggccacgacc
      721 tggctctaag tctaactctg ttcggcgaag gtcttccggc cgagccgctg cgagagcggt
      781 caggagaccg ctcccgaacc ggaaacagta gtgactctaa ctccatgctc actgtgcaga
      841 atctcaagaa atactacccg atccggcgcg gcgtgttccg gcagcatgcg ggcgacatca
      901 aggccgtcga cggcgtgagc tttacgatca ggcgagggga gacgctgggt ctggtgggcg
      961 agagcggctg cggcaagtcg acgacgggcc ggtcgatctt gcagctggag aggccgacgg
     1021 cgggcgaggt gatcttcgag gggcgtgaac tgacgaagct gcgcggcgaa gagctgcgcc
     1081 gcatgcgccg ccacatgcag atcattttcc aggatccgta tgcgtcgctc aacccgcgca
     1141 tgacggtggg cgagatattg aatgagccgc tgcaggcgca cggcgccgcc gccagcgggg
     1201 cgaggcgcga gcgcgtccgc gagctgctca gcctggtcgg cctggatccg tcgttcgccg
     1261 cgcggtttcc ccacgaattt tccggcggcc agcgccagcg catcggcatc gcgcgcgcgc
     1321 tggcgctgaa tcccctcttc gtcgtgtgcg acgagccgat cgccgcgctg gacgtgtcca
     1381 ttcaggcgca gatcgtcaat ctgctgaaag acctgcaggc gcgcttgggg ctgacctacc
     1441 tgttcatcgc gcacgacctg tcgatggtgc ggcacatcag cgaccgggtg gcggtgatgt
     1501 atttgggacg cctcgtggag atcgccgatt ggggggacgt ctatgccgat ccgaagcatc
     1561 cctatacgca ggcgctgctg tcggccatcc ccatccccga tccgaaagtc gaggcgagac
     1621 gccagcgcat cgtcctggag ggcgacgtgc cgagtgcggc cagtcctccc aaaggctgcc
     1681 acttcaacac acgctgcccg atccgacagg agcgctgcta tcacgaagat cccgagctgc
     1741 gcgagatcgc gccggggcac tgggcggcct gccattttgc ggcgtaagga atgtgttttc
     1801 gcgcaccgga aggaggtgaa cgttatcata ctcactcagc gtcatacgag gcactgcatg
     1861 gagggctggt aagtctattt cagtttccac tctggaggag aagaaaatga atcgcaaatt
     1921 cgcaatgacc gggatcctga tcctggccgt actcgcgtct gcatgtgcgc ccgcatcgac
     1981 cccaaccgcc ccacccgcta cccaggcgcc gggagcggcg gaaccaccgc aagtggagcc
     2041 gacgaagccg gcggagccga ctgccgcgcc ggccgcgccc gagccgaagg acaaggagac
     2101 cttcgtccag
//
LOCUS       DBNHXB010000009         7827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2853639, whole genome shotgun sequence.
ACCESSION   DBNHXB010000009 DBNHXB010000000
VERSION     DBNHXB010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2853639"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..1284)
                     /locus_tag="AC162G_00275"
     CDS             complement(283..1284)
                     /locus_tag="AC162G_00275"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR5074280.1"
                     /translation="MSSTGASNNGAVTALLSAMLILAVIVLVGVAVVATPTFTESAEA
                     EPGPPLVDASTVVPTITPPPTWTPTSTSTPADTPLPSATPEPSLTPTPALGSRPQPDG
                     VQRTARVPILMYHYISAPPDPGDRLRVDLSVLPENFDAQMRWLAENNYHTITLADLYT
                     HLAVGAPLPENPIILTFDDGYVDHYENAFPILQKYGFTGAFFVLAGPADVASPRYLTW
                     EMIAEMSATGQDMQVHGRAHVDLRRRSGEFLFFQIVGARQAIEAHTGKPAPFFAYPAG
                     QYDADVLRFLGERDFWMAVTTNYGRDHSLDSALELGRVRIRGGDSLQRFISKVTTGK"
     gene            1274..1945
                     /locus_tag="AC162G_00280"
     CDS             1274..1945
                     /locus_tag="AC162G_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074281.1"
                     /translation="MDDTGEGYPGDGGVSRRAWRIAVAVLGMIIGVTGLAAIAIAIRP
                     DVRRAAPIWSDDFDRASREWTIDPGDGEAAAGHLHLHPTRPGASALATHALPVDDFVV
                     ETQAGVVAGSPDNGYGIVVGRADEMTAFLVGGDGYFGVQRRIDGRWSEAQPWRQWPHV
                     RRGGAVNTLRVECHAAACAFYVNDELTAHDAVAHEWSLIGAMAQRLSEERLEVEFDYL
                     KVWQR"
     gene            2052..2249
                     /locus_tag="AC162G_00285"
     CDS             2052..2249
                     /locus_tag="AC162G_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074282.1"
                     /translation="MFDDLRRAEVDPNVRYEEASPSLVERLPVFSVLKPQQRFVLALL
                     LFMNVTVLGCGCLLAFDRLIL"
     gene            2256..2948
                     /locus_tag="AC162G_00290"
     CDS             2256..2948
                     /locus_tag="AC162G_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5074283.1"
                     /translation="MAETILIIEDDPMQCDVVQDLLQLEGYTSVAAMDGPEGLRKLYE
                     TQPDLVILDVMLPTMDGWEVCRRIRELSTVPVIIMTSRTSDEEKVKGLRLGADDYIVK
                     PFNPKELGARVNAVLRRTRLPPPAKSNIMRFAGGDLIVDPKSPTVIRDGKPIELTPTE
                     HRILMYLVEHPGRVVSVDDIFTAVWGFETEVNLNNVKWYIWRLRQKLEGDAEQTRFIF
                     TERGVGYRFTAV"
     gene            3119..4321
                     /locus_tag="AC162G_00295"
     CDS             3119..4321
                     /locus_tag="AC162G_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074284.1"
                     /translation="MHRFLIPTRRVTLILLFLGLLLSLYAGSAAAAYFDSAPHSITEN
                     GECLTKGTDDRPPDLVVFLRVLPREQVAQVVVVNRSESNCYNVGLAAYERQSPSSAPM
                     YFSNAASAVPPGQSRSLQVDVPCRGDAYLFLGPAIVNAPIDYGDRLLRVKPFLIPRPY
                     CHPQPLLTATPTPTPTDTATPTPTDTATPTATDTATLTPTDTVTPTPTDTSTPTDTPT
                     PTPTETATSTPTDTATPTPTNTTTPQAITSKSAVVYRDGPTTPEPGVVQPGDRIVYTV
                     WFTNTGAVDILQARISDVIPNDTTYLPGSASSNGSLTIGNPLVSVMDPLTPGQVFTLT
                     FTVIVNQTPQATQIVNLAIINALNIDPPDPSLILLVDTGGTAIPDIIGSAPRKTIYLP
                     VRPPDLGD"
     gene            4342..5028
                     /locus_tag="AC162G_00300"
     CDS             4342..5028
                     /locus_tag="AC162G_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5074285.1"
                     /translation="MPERVLVIDDDPAHVRMIELALLDARFQPVTATSGEEGLRKLLA
                     YRPDLVILDVIMPGMDGLETCARIRQVSTVPVIFLTAKQSIDDKVAGLQLGGDDYLVK
                     PFSPGELIARVEAVLRRSHRPLAERDAVLRVGGRLVIDRGTRQVVVRNQSVSLRPAEY
                     NLLLILAERAGQVVSAEQIGELMGITDPGARPRRVKWHIWKLRQSIESDPRKPELVLT
                     RRGGYLLANS"
     gene            complement(5121..6332)
                     /locus_tag="AC162G_00305"
     CDS             complement(5121..6332)
                     /locus_tag="AC162G_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR5074286.1"
                     /translation="MSNSEFAGLSLTPAGDAPGTSSRECSGDTAVPSLSPGWSMVWQQ
                     VFEHPIALPPEVTDTQLILLERADPFPGEFRDTVWVLNPQTGAVLWRFDGADNAALPV
                     RWIKNMAWSQKYIAVNVHYQKGLRFEIPEEYLVVLDRSSGEVVYTEQVHVLGMTVSDG
                     AVYYRGEWRELNRVDLPAGEHRWRMPWKGSFPRGDFVIGSWLYSFNLDRNVYKLNAAD
                     GALVATATLGIPPILGDVLIEDQRAVIRSERDGVVLFDLQALRPRWATHVDYLIAKDS
                     NAFWGDIPSMTLTPNALHLFDAMDNLLQVELASGHIVWRIPSPGAQAISRPIVFHDLV
                     YGLYADGTLRAYSAADGSPVGTVMKVPLWYRIRSDAWAWRSLVGGLDATSDTLIATTG
                     CRSVYAIQRGN"
     gene            complement(6677..6928)
                     /locus_tag="AC162G_00310"
     CDS             complement(6677..6928)
                     /locus_tag="AC162G_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074287.1"
                     /translation="MTLTFTGTWAGFGYLAAPLVQVLVQEEAGGKTAYLYGVTRVGEQ
                     QPAGWVYHLPDALGSVRQLVDNSVQVVLARGNEPRRQRI"
     gene            7088..>7827
                     /locus_tag="AC162G_00315"
     CDS             7088..>7827
                     /locus_tag="AC162G_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR5074288.1"
                     /translation="MADRVPAVDRALRILNAFKDGHAEYGVTDLSRLLGINKSTVHDI
                     LHTLAHYNLLERNPATQKYRLGPGLMEFGDLARQRRDVHEVAHPFLRPLMEATTLTAL
                     LGVFEADSITIVDKVEPPDEMRIAASVGQRMPFCAGSFGRAFLAWIDEASVDRLLVSP
                     GLRKFTATSITDPAAYKASLASVRRQGYAVDDTEEYLEGAWAASAPIRDATRVVAAMT
                     VVGFTGRISAARKKTAIRALLQTTRRIS"
BASE COUNT         1555 a         2449 c         2325 g         1498 t
ORIGIN      
        1 acaaaaagcc cgggccttat ggcgtgcggg ctgagccatc tggtgcataa tatagcatca
       61 gtctgccttc aaaaactatc aggcctgatt cttgagggga tttgagacga tctcgctact
      121 gcacaagcgc acgaaggggc gcaagagttc gatacacact cacctggatt cttcgagtag
      181 acctggcagg tcttcgccaa acatccgttg acaagacttg ccaggtctat agagatcatt
      241 agattgtgag agcaagatga gaaaagaaga agagaggaag aattacttgc cagtcgtcac
      301 tttgctgatg aacctctgca gggagtctcc accccggatg cgaacgcggc ccaattccag
      361 ggcgctatcg agactgtgat cgcggccata attcgtcgtg accgccatcc agaaatcgcg
      421 ctcgcccagg aagcgcagca cgtcggcgtc gtactgcccg gcggggtagg cgaaaaacgg
      481 cgcaggtttt ccggtgtgcg cttcgatcgc ctgccgcgcg ccgacgatct ggaagaacag
      541 aaactcgccg gatcggcggc gcaggtcgac gtgggcgcgg ccgtgcacct gcatgtcttg
      601 tccggtcgcg ctcatctcgg cgatcatctc ccaggtgaga tagcgcggcg acgcgacgtc
      661 cgccggcccg gccaggacga agaaggcgcc ggtgaagccg tacttctgga gaatgggaaa
      721 ggcgttttcg tagtgatcga cgtagccgtc gtcgaacgtg agtatgatcg ggttctccgg
      781 caagggcgcg ccgaccgcca gatgggtgta caggtcggcg agcgtgattg tgtgataatt
      841 gttctcggcc agccaccgca tctgggcgtc gaagttttcg ggcaggacgg aaaggtcgac
      901 gcgcagccgg tcgccgggat cgggcggcgc cgagatgtag tggtacatca gaatgggcac
      961 gcgcgccgtg cgctgcacgc cgtcgggctg cggccgggat cccagtgcgg gagtaggcgt
     1021 caggctcggc tccggcgttg cgctcggcag aggtgtgtcg gccggcgtcg atgtggatgt
     1081 gggcgtccag gtcggtgggg gagtgatcgt cggcacaacg gtggacgcgt cgaccagcgg
     1141 cggtcccggt tcagcctcgg cgctctccgt aaacgtcgga gtcgccacga cggcgactcc
     1201 cacgagcacg atcacggcga ggatcagcat cgcgctgagg agcgctgtga ccgcgccgtt
     1261 gttggaggct ccagtggacg acacgggcga aggatacccc ggcgatggcg gtgtgtcaag
     1321 gcgcgcctgg cgaatcgccg tcgccgtgct gggcatgatc atcggcgtga ccggcctggc
     1381 ggcgatagcc atcgccatcc ggcccgacgt tcgtcgcgca gcgccgatat ggtcagacga
     1441 tttcgatcgc gcatcgcgcg agtggacgat cgatccgggc gacggggagg cagccgcagg
     1501 ccatctccac ctgcatccaa cccggcccgg agcgagcgcc ctggcgacgc acgccctgcc
     1561 ggtcgacgat ttcgtcgtgg aaacgcaggc cggcgtcgtc gccggatcgc ccgacaacgg
     1621 ctacggaatc gtggtcggaa gagcggacga gatgacggct tttctggtcg gcggcgacgg
     1681 ttatttcggt gtgcagcgcc gcatcgacgg tcgctggtcc gaggcgcagc cgtggcggca
     1741 gtggccgcac gttcggcgcg gcggggccgt caacacgctg cgtgttgaat gccacgccgc
     1801 agcgtgcgca ttctacgtga acgacgagtt gacggcgcac gacgcggtcg cccacgagtg
     1861 gagccttatc ggcgcgatgg cgcagcgact gtccgaggag cgcctggagg tcgagtttga
     1921 ctatctgaag gtgtggcaga gatgaggttg gaaggtggtt acaactcccc tcaaaccatt
     1981 tgacttttac gcgtgcctca ggtatcctgg taggtggccg cacaagagat acgcagcttt
     2041 ccggagggct tatgtttgac gatctgcgca gagcagaggt cgatccgaat gtgcgctacg
     2101 aagaggcgtc gccgtcgctc gtcgagcggc tgcctgtctt ttcggtgctc aagcctcagc
     2161 agcggtttgt cctggcactg ctgctgttca tgaacgtgac cgtcctggga tgcggatgcc
     2221 tgctcgcttt cgatcggttg attctgtgag cggccatggc tgagacgatt ctgatcatcg
     2281 aagacgatcc gatgcagtgc gacgtcgtgc aggacctgct gcagctcgaa ggctacacga
     2341 gcgtcgcggc gatggacggc ccggaggggc tgcgcaagct gtacgaaacg cagcccgacc
     2401 tggtgatcct ggacgtcatg ctgccgacga tggacgggtg ggaagtgtgc cgccgcatcc
     2461 gcgagctgtc taccgtgccg gtgatcatca tgacctcgcg caccagcgac gaagagaagg
     2521 tcaagggcct gcggctgggc gcggatgatt acatcgtcaa gcctttcaac ccgaaggagc
     2581 ttggcgcgcg agtgaacgcc gtgctgcgcc gcacgcggct gccgccgccg gccaagagca
     2641 acatcatgcg cttcgcgggc ggcgacttaa tcgtcgaccc gaaatcgccc accgtcattc
     2701 gcgacggcaa gccgatcgag ctgacgccca ccgagcatcg tatcctgatg tacctggtcg
     2761 agcaccccgg ccgcgtcgtc tccgtagacg acatcttcac cgccgtgtgg ggattcgaaa
     2821 cggaggtgaa tctcaacaac gtgaaatggt acatctggcg gctgcgccag aagctcgagg
     2881 gcgacgcgga gcagacccgt tttatcttca ccgagcgcgg cgtgggatat cgattcacgg
     2941 cagtctagcc gcaacagagg ttggaaattg ggatgtggtg tatatggtag ggttctgcat
     3001 gcttggtcgt ctattttcta accattgacg cttgctaact caaaccggtc tcttaccaaa
     3061 ggagtcctat gtatttggta gaatgtatgc attatcggct ccggaggcaa gaacgccggt
     3121 gcacaggttt ttgattccaa ctcgccgtgt tacacttatc ctcctattcc tgggcctgct
     3181 gctgtcgctg tacgccggca gcgccgccgc tgcttatttc gattccgcgc cccacagcat
     3241 taccgaaaat ggcgagtgcc tgaccaaggg gaccgatgat cgcccgcccg atctcgttgt
     3301 gttcctccgg gtcctcccgc gcgaacaagt cgcccaggtt gtggtggtta atcgctccga
     3361 gtccaactgt tacaacgtcg gcctcgcagc ctacgagcgg caaagcccct cgagtgcgcc
     3421 gatgtatttc tccaacgcgg cgtcagccgt gccgcccggg caatctcgct cgctgcaagt
     3481 ggacgtgccc tgtcgcggtg acgcctactt gttcctcggt cccgcaattg taaacgcgcc
     3541 gatcgattac ggcgatcgcc tgcttcgagt gaagcccttc ctcatccccc gcccttactg
     3601 ccatccacag ccattactta ccgcgacgcc cacaccaacg ccgaccgata ccgcgacccc
     3661 gacgcccact gacacggcta caccgacagc aaccgacacc gcgaccctga cgccgacgga
     3721 cacggttaca ccgacgccga ccgacacgag cacgccgaca gatacgccga cgccgacgcc
     3781 gaccgagacc gcgacctcca cgccgacgga cacggcgacg ccgacgccca cgaacacgac
     3841 cacgcctcag gccatcacga gcaaaagcgc tgtcgtctac cgcgacgggc cgaccacgcc
     3901 ggagccaggc gtggtgcagc cgggcgaccg catcgtgtac acggtgtggt tcacaaatac
     3961 cggagcggtc gatattctcc aggctcgcat cagcgatgtg atcccgaatg acacgaccta
     4021 tctgcctggc agcgcatcca gtaacggcag cctgacgatc ggcaatccat tggtgtcagt
     4081 gatggatccg ttgaccccgg gtcaggtctt cacgctcacc ttcaccgtga tcgtcaacca
     4141 gacgccgcag gccacgcaga tcgtcaacct cgccatcatc aacgcgctga acatcgaccc
     4201 gcccgacccc tccctgattc tcctcgtcga cactggcgga actgcgattc ctgatataat
     4261 aggtagtgcg ccgaggaaga ctatctacct gcccgtccgt ccacctgacc tcggcgactg
     4321 agattctcag agcgcctggg aatgcctgaa cgagtcctcg tcatcgacga cgatccggcg
     4381 cacgtgcgca tgattgagct ggcgctgctc gacgcccgct tccagccggt cactgcgacg
     4441 agcggcgaag aaggcctgcg caagctgctc gcgtatcgcc cggacctggt catcctcgac
     4501 gtgatcatgc cgggcatgga cggcttggag acgtgcgctc gcatccgcca ggtctccacg
     4561 gtgcccgtca tcttcctcac cgcgaagcag agcatcgacg ataaagtagc cggcttgcag
     4621 ctcggcggcg acgattacct ggtcaagcct ttcagcccgg gcgagttgat cgcgcgcgtg
     4681 gaagccgtgc tgcggcgctc gcaccgcccg ctcgccgagc gggacgcggt tctccgggtc
     4741 ggcgggcgcc tggtcatcga tcgcggcacg cgccaggtcg tcgtgcgcaa tcagtcggtg
     4801 tcgctgcgcc ccgccgagta caacctgctc ttgatcctgg ccgagcgcgc cgggcaggtg
     4861 gtcagcgccg agcagatcgg cgagctgatg ggcatcaccg atccgggcgc gcgcccgagg
     4921 cgcgtcaagt ggcacatctg gaagctgcgc caatcgatcg agagcgatcc ccgcaagccc
     4981 gagctggtcc tcacgcggcg gggcggatac ctgctggcca actcttaacg ggtgtgggct
     5041 cgtctagcga ctatccgcac atgccagtgg tagacggtgt cggcggaagg ccagcgacgt
     5101 tctgcgtgat gacgacgccg ctagtttccg cgttggatag cataaacgct acggcagcca
     5161 gtcgtagcaa tcagtgtatc acttgtggca tccaatccac caaccaaact acgccacgcc
     5221 cacgcatctg accttatccg ataccatagc ggtaccttca tgaccgtacc tacaggtgac
     5281 ccatctgctg ctgagtaagc acggagtgtt ccgtcagcgt ataaaccata caccagatcg
     5341 tgaaatacaa tgggtctgga tattgcctgc gctccgggcg atggaatgcg ccagacaatg
     5401 tgaccgcttg ccaattcgac ttgcagcaag ttgtccatgg catcaaagag atgcaacgcg
     5461 ttgggggtta gagtcatcga ggggatatca ccccaaaatg cgttggagtc ctttgctatc
     5521 aaatagtcga catgggtcgc ccatctgggt cgcagggctt gcagatcgaa caaaacgacg
     5581 ccgtcgcgtt ctgatcggat aacagcacgt tgatcctcaa tgagcacatc accgagaatg
     5641 ggcggtatcc ccagtgtcgc ggttgcgacc aatgcaccat ccgctgcgtt gagtttataa
     5701 acgtttcgat caagattgaa tgaatataac caggagccga taacaaaatc acctctaggg
     5761 aaacttcctt tccatggcat gcgccatcgg tgctcacctg cgggcaagtc aactcgattt
     5821 agctctcgcc attcaccacg ataatacact gcaccgtccg acacagtcat tcctaggacg
     5881 tgcacctgct cagtgtagac aacttcccca gacgatctat ccagcaccac caaatactct
     5941 tccggaattt cgaacctgag ccctttttga tagtgaacat tcactgcaat atacttctga
     6001 ctccaagcca tgtttttgat ccaacgcaca ggcagcgcag cattatcggc gccatcgaat
     6061 cgccataaaa cagcacccgt ctgcggattt agtacccata cggtgtccct gaactcaccc
     6121 ggaaaagggt ctgctcgttc cagaagaatg agttgcgtgt cagtgacttc aggcggcaac
     6181 gcaatggggt gctcaaaaac ctgctgccag accatgctcc agccaggaga taacgagggg
     6241 actgccgtat ccccgctgca ctctcgactc gacgtgcccg gagcatcccc agctggcgtc
     6301 aaggataatc cagcaaattc ggaattgctc atcgatgtac tcgtaggtgg aatctcgggc
     6361 gagtaatcga gacttgtaca actgattagg ctgatgcaga ggataaggat acgcgctatg
     6421 tgcctgttaa cttcgatgct cttcacccca attccgtaat tctccataca cagtcacccc
     6481 ttctcatttc ggaccatgct gcattatgta ggttactgca ttattaagtt catccactgt
     6541 agcatcatgt tcttcttcaa gcggtacgtg gcttgctacc atttgcatga ttgtcccaag
     6601 tgaaggaccc gagattgata ccgtcccttg taaaaccaca gacaaccata tcggtccagc
     6661 cgtgttgaat gtgacgtcat atgcgttgac ggcggggttc gttgccgcgc gccagcacga
     6721 cctgtacgct attatccacc aactgccgca cgctgcccag cgcgtcgggc aaatgataca
     6781 cccatcccgc cggctgctgc tcgccgaccc gcgtcacacc gtacagatat gccgtcttgc
     6841 ctcctgcctc ctcctgcacc agcacctgca ccagcggcgc agccaggtag ccgaagccag
     6901 cccacgtgcc ggtgaaggtt aacgtcaccg tctggttggc ggtgtcgctc cagacgccgg
     6961 tcgctatccg ccatcagcta tccgctaccg gccctccgca actcccgctt gacaagcgcg
     7021 ccgatttcaa atatactgtt gttgtctata gcgaacgtcg ttcggcatat agaacaggct
     7081 atgacccgtg gctgaccgcg tccccgccgt cgatcgcgcg ctgcgcattc tgaacgcctt
     7141 caaggacggc cacgccgaat acggtgtgac cgatctcagc cgcctgctgg gcatcaacaa
     7201 aagcaccgtc cacgatattc tccacactct tgcacattac aacctgctcg aacgcaaccc
     7261 ggccacgcag aagtaccgcc tcggccccgg actgatggaa ttcggcgacc tggcccgcca
     7321 gcgccgtgac gtccacgaag tggcgcaccc gttcctgcgc ccgctcatgg aggcgacgac
     7381 cctaactgcc ctgctggggg tcttcgaggc ggactcgatc acgatcgtcg acaaggtcga
     7441 gccaccggac gagatgagga tcgcggcgtc ggtcggccag cgcatgccgt tttgtgcagg
     7501 ctccttcggc cgcgcatttc tggcctggat agacgaggcc agcgtggacc gcctgctggt
     7561 atcgccgggc ctgcgtaagt tcaccgccac gtcgatcacc gatccggctg cttataaagc
     7621 cagcctggcc tcggtccggc ggcagggtta cgccgtcgac gacacggagg aatacctgga
     7681 aggcgcgtgg gccgcctcgg cgccaatccg cgacgccact cgcgtcgtcg cggcgatgac
     7741 cgtcgtcggc ttcaccggcc gcatcagcgc ggcccgaaag aagacggcga ttcgcgcctt
     7801 gcttcagacg acgcgccgca tctcgca
//
LOCUS       DBNHXB010000010         5370 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_505904, whole genome shotgun sequence.
ACCESSION   DBNHXB010000010 DBNHXB010000000
VERSION     DBNHXB010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_505904"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..97)
                     /locus_tag="AC162G_00320"
     CDS             complement(<1..97)
                     /locus_tag="AC162G_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017475194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5074289.1"
                     /translation="MLVVEDIHTYYDESHVLQGLSLTVNQGEVIAL"
     gene            complement(97..864)
                     /locus_tag="AC162G_00325"
     CDS             complement(97..864)
                     /locus_tag="AC162G_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5074290.1"
                     /translation="MSRLKADNLRKNFGGIAALAGVTLEVEPGERRAIIGPNGAGKST
                     LFNLIAGELPPAGGRVYFDGRDMTGLSVDERANLGVGHTFQRNNLFPGLTTFQNARLA
                     VQHRRGVARHAFRDARRFDAIERETGELLSRVGLAEQRDQVVGTLAYGQQRALEVALA
                     LATGPKVLLLDEPTAGMSPAETADMVRLIESLPRSLTMLIVEHDMDVVFSLADRITVL
                     HYGEVIAEGTPGDVRANPLVQEIYLGAREAQALTGES"
     gene            complement(861..1919)
                     /locus_tag="AC162G_00330"
     CDS             complement(861..1919)
                     /locus_tag="AC162G_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5074291.1"
                     /translation="MGRLTARLSAALLWLNRHRRWVSIAALVFAFAYPSIIARIYFVT
                     LAEDVLIYAIFAMSLDLLLGYAGLISFGHAAFFGVGAYILAYLTTRFGVTNLLVSVPV
                     VLLGTAAVALLIGFFALRTRGIYFLMVTLAFAQMLFSIAINWTVATGGSDGLAGVPRP
                     SLGVGPFSYTFDSRESFYYLVLVFFLLTFWLLRRVVSSPFGWTLRGIRENEARMRALG
                     YNTFRFKLASFAIGGAFAGLAGLLLAHSARIASPENLYWTTSGQVMIMVIVGGAGTLS
                     GPVFGAATVRLLRNFISSATPRWETLMGVIFILFVIFARRGIMGMLSDARKRFVVEYP
                     ASAARPAEISESPQVEAE"
     gene            complement(1922..2803)
                     /locus_tag="AC162G_00335"
     CDS             complement(1922..2803)
                     /locus_tag="AC162G_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5074292.1"
                     /translation="MGVTVMENFLIQLLNGLVSGMLLFVMAAGLSLIFGLMDVINLSH
                     GALYLLGGYIGLTMVRQLDNYWLALLAAPLLVGSLGMIIQYLFLRRLHGRQRHLDQVL
                     FTFGVALITIDLSRATWGAYVESVSPPALLTGLVPVLTFEFPVYRLALLGFGLALALA
                     LWLFIERTRLGAIVRAGVSDAEMVGGLGINIQLVFAGVFALGTALAGLAGVVGAPVLN
                     LYPGLDSEILILALVVVVVGGLGTLKGAFFGSLLIGLADTFGKALLPEFARFLIFAVM
                     AAVLLIRPSGLFGQKGS"
     gene            complement(2857..4083)
                     /locus_tag="AC162G_00340"
     CDS             complement(2857..4083)
                     /locus_tag="AC162G_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5074293.1"
                     /translation="MTTQTKSRLTRRQFLKAAGLTAGATAASPLLSACGPAAPAGPVK
                     IGVLLPYSDIYAALGESITAAMEMYFEEVGGTAGGRQLEVIKEDEGTAPDAALQKARK
                     LVEQDEVAFLTGIVSSGVLNALRDYVDANKKLLICSNAGANPLSRGAKTRYIWRTSFT
                     NWQPNWPLGTWAYENVAQQVFVSVPDYAAGHNMRDAFLNSFQAAGGEVLGTQLTPFPN
                     MGDPAPFITEIQSADPPMVYCFYSGGAAVTFVKAYGEFGLSGNIPLVCAGFTVEEDVL
                     PAQGDAALGARSGLHWSLLLDNPENQKFTAAYKQKTGKDANVFAVQGYDTGRVIVDML
                     NAVQGDTSNVDKMIDALGGVSFASPRGPFALDANSQNPRQHIYVREVQNVEGVLHNVV
                     IADLGDIADPGDDSKG"
     gene            complement(4303..5121)
                     /locus_tag="AC162G_00345"
     CDS             complement(4303..5121)
                     /locus_tag="AC162G_00345"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008388782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR5074294.1"
                     /translation="MDTKGEPGGEDVREGEGEPRPYTAAELMVAVAAREINDGDVVFV
                     GMRLPLLGFQLAKQAHAPNAVGVFELGIVRDAPAPEPIFTMGDLPNLHRATWLADTGD
                     VMGLLQQGLVDVSFIGGAQVDRFGNLNTSYIGGPDQIETRLPGSGGACDLASLARRHI
                     IIMAHEKRRFVPKVDYITSPGYGDGAGWRERAGLPRGGPSTVITTLAILRFDPETREM
                     ILSSVHPGTTIEDVMAHTGWLLKVAASVAETPAPTEDELSILRRFDPQGFWTGA"
     gene            complement(5111..>5370)
                     /locus_tag="AC162G_00350"
     CDS             complement(5111..>5370)
                     /locus_tag="AC162G_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR5074295.1"
                     /translation="RVSAVAHAPWGAHPSPVPGHYNRDHQMFLDYQEESQTPEAFVEW
                     QARWVDGVRSSEDYVARLGEERVAGLRLKHHAPAEAVDYGY"
BASE COUNT         1005 a         1787 c         1715 g          863 t
ORIGIN      
        1 gcagcgcaat cacctcgccc tggttcaccg tcaacgaaag cccttgcagc acgtggctct
       61 cgtcgtagta cgtgtgaatg tcctcgacga ccagcattaa gactcccctg taagcgcctg
      121 cgcctccctg gcgcccagat aaatctcctg caccagcgga ttggcgcgca cgtcgcccgg
      181 cgtgccctcg gcgatcacct cgccgtaatg cagcacggtg atgcgatcgg ccaggctgaa
      241 cacgacgtcc atatcgtgct ccacaatcag catcgtgagc gagcgcggca gcgattcgat
      301 caggcgcacc atgtcggctg tctcggcggg tgacatgccg gccgtcggct cgtcgagcag
      361 aaggaccttc ggcccggtcg ccagcgccag cgccacctcc agcgcgcgct gctggccgta
      421 agccagggtg ccgacgacct gatcgcgctg ctcggccagg ccgacacggc tgagcagctc
      481 tccggtttcg cgctcgatcg cgtcgaagcg gcgcgcatcg cgaaacgcgt ggcgcgccac
      541 tccgcgccga tgctgcacgg ccagccgcgc gttttggaaa gtggtcaggc ccgggaacaa
      601 gttattacgc tggaaggtgt ggcccacgcc caggttcgcc cgctcgtcga cggagaggcc
      661 ggtcatgtcg cggccatcga agtaaacgcg gccgccggcc ggcggcagct cgccggcgat
      721 caggttgaac aaggtgctct tgcccgcgcc gttcgggccg atgatcgcgc gtcgctcgcc
      781 cggctctact tccagcgtca cgccggcgag tgccgcgatg ccgccgaaat tcttgcgcag
      841 gttatccgct ttcaggcggc tcattcggcc tccacctgcg gcgactctga aatttcagcc
      901 gggcgcgctg cggacgccgg atactcgacc acgaatcgct ttcgagcatc cgacagcatg
      961 cccatgatgc cgcgccgcgc gaatatcacg aacaagatga agataacgcc catcagcgtt
     1021 tcccagcgcg gggtggcgct gctgatgaag ttgcgcagca gccgcaccgt cgccgcgccg
     1081 aagaccggcc cgctcagcgt gcccgcgccg ccgacgatga ccatgatcat cacctgtccg
     1141 gacgtcgtcc agtagagatt ctccggcgag gcaatgcgcg ccgaatgagc gagcagcagg
     1201 ccggccaggc cggcgaatgc gccgccgatc gcgaacgagg cgagcttgaa tcggaacgtg
     1261 ttatagccca gcgcgcgcat gcgcgcctcg ttctcgcgga tgccgcgcag cgtccacccg
     1321 aacgggctgc tcacgacgcg gcgcagcagc cagaaggtga gcagaaagaa gacgagcacc
     1381 agatagtaaa agctctcgcg gctgtcgaac gtgtaactga acgggccgac gccgaggctc
     1441 gggcggggca cgcccgccag cccgtccgag ccgccggtgg ccaccgtcca gttaatcgca
     1501 atgctgaaca gcatctgggc gaaagccagc gtgaccatca ggaaatagat tccccgggtg
     1561 cgcaatgcga agaagccgat caacagcgcc actgcggccg tgccgagcaa gacgaccggc
     1621 acgctgacca gcagattggt cacgccgaat cgtgtcgtga ggtaggccag gatgtacgcc
     1681 cccacgccga aaaaagcggc gtgtccgaac gagatcaggc cggcgtatcc caacagcagg
     1741 tcgaggctca tcgcgaagat ggcgtaaatc aggacatctt ctgcgagtgt cacaaagtag
     1801 atgcgagcga tgatggaggg ataggcaaag gcgaagacga gagcggcgat cgatacccat
     1861 cgacgatgcc ggttcagcca cagcagtgcg gcggacaggc gcgccgtgag acgccccatc
     1921 gctagcttcc cttctgcccg aacaacccgc tggggcggat caagagcacc gccgccatca
     1981 ccgcgaagat caagaagcgc gcgaattccg gcagcagtgc cttgccgaac gtgtcggcca
     2041 ggccgatcag aagactgccg aagaacgcac ccttgagcgt gccgaggccg ccgaccacga
     2101 cgacgacgag ggcgaggatc aggatctccg agtcgaggcc gggatacagg ttgagcacgg
     2161 gcgcaccgac tacccccgcc aatccggcca gcgccgttcc gagcgcgaag acgccggcga
     2221 acactaactg aatgttgatg cccaagccgc cgaccatctc cgcatccgac acgcccgcgc
     2281 gcacgatcgc ccccagccgc gtgcgctcga tgaacagcca cagcgccagc gccagtgcca
     2341 ggccaaagcc gagcagcgcc aggcggtata ccggaaactc gaacgtgagc accggaacca
     2401 gcccggtcag cagcgcgggc ggcgacaccg attcgacgta tgcgccccac gtcgcgcgcg
     2461 acagatcgat cgtgatcagc gccacgccaa acgtgaatag cacctggtcg agatggcgct
     2521 ggcggccgtg taagcgccgc aggaataaat actggatgat catgcccaga ctgccgacca
     2581 gaagcggggc ggccagcagc gccagccagt agttatcgag ctggcggacc atcgtcaggc
     2641 cgatgtagcc gcccagcaga tagagcgcgc cgtgcgacag gttgatgaca tccatcaagc
     2701 cgaagatgag cgaaagcccg gcggccatca cgaacagcag catgccggac accagcccat
     2761 tgagcagttg gatcaggaag ttttccatga cggtgacccc cacccgcacc ctcccctgtc
     2821 aaagacgggg gagggtgcgg gtgggggtag gtaaggctag cctttggagt cgtcgccggg
     2881 atcggcgatg tcgcccaggt cggcgatgac gacgttgtgc agcacgccct cgacgttctg
     2941 cacctcgcgc acgtagatgt gttggcgcgg gttctgagaa ttggcgtcga gcgcgaacgg
     3001 gccgcgcggg ctggcgaagc tgacgccgcc caacgcgtcg atcatcttgt cgacgttgct
     3061 ggtgtcgccc tgtaccgcgt tgagcatgtc gacgatgacg cgcccggtgt cgtacccctg
     3121 cacggcgaag acgttggcgt ccttgccggt tttctgcttg taggccgcag tgaacttctg
     3181 gttctccggg ttgtccagca gcagtgacca gtgcaggccg cttcgcgcgc ccagcgccgc
     3241 gtcgccctgg gccggcagca catcctcttc gacggtgaag ccggcgcaga ccaggggaat
     3301 gttgccgctc aggccgaact cgccataggc cttgacgaac gtgaccgccg cgccgccgct
     3361 gtagaagcag tacaccatgg gcgggtcagc gctctggatt tcggtgatga acggggccgg
     3421 atcgcccatg ttcgggaacg gcgtgagctg ggtgcccagc acctcgcctc cagccgcctg
     3481 gaaactgttc aagaaggcat cccgcatgtt atggccggct gcatagtccg gcacgctgac
     3541 gaacacctgc tgggcgacgt tttcataggc ccaggtgccc agcggccagt taggctgcca
     3601 gttcgtgaac gaggtgcgcc agatgtagcg ggtctttgcg cctcggctca gggggttcgc
     3661 gccggcgttc gagcaaatca gcagcttctt gttggcgtcg acatagtcac gcagggcatt
     3721 cagcacgccc gagctgacga tgccggtgag gaaggccacc tcgtcctgct ccaccagctt
     3781 gcgcgccttc tgcagcgccg catccggcgc ggtgccctcg tcctctttga tgacttcaag
     3841 ctggcggccg ccggccgtgc cgccgacctc ttcgaagtac atctccatcg ccgcggtgat
     3901 gctctcgccg agggcggcgt aaatgtccga atacggcagc agcacgccga tcttcaccgg
     3961 accggcaggg gcggccgggc cgcacgcgct gagcaggggt gaagcggcgg tcgcgccggc
     4021 cgtcaggccg gcagccttca ggaattggcg gcgtgtcaac ctcgacttcg tctgggtggt
     4081 catgggtgtt ctcctccgat aggtggacgc agcttcggac gaaccggatc gacaagcacg
     4141 ggagtcagac ccgacgtggc ccacgtgtga cgcttgacgg catcacctcc ttctctctct
     4201 gacttggcag ttccggctct tcgaggtttg gcgagaaaac atccgcattg tagcatagta
     4261 tgctacccac gaatgagcag ggagcaatcg cagtcaacga cctcacgctc cggtccaaaa
     4321 tccctgcgga tcaaaccgtc tcagaatcga caattcgtct tcagtcggcg caggcgtctc
     4381 cgcgacgctc gccgcaactt tcaggagcca gcccgtatgg gccatgacgt cttcgatagt
     4441 ggtgcccggg tgaacggatg acagaatcat ctcgcgcgtc tcgggatcga agcgcaggat
     4501 ggcgagtgtc gtgatcaccg tcgaggggcc gccgcgcgga aggccggccc gctcgcgcca
     4561 gccggccccg tcgccgtagc ccggcgaggt gatgtagtcg accttcggca cgaagcggcg
     4621 cttctcgtgc gccatgatga tgatgtgccg ccgggccagg ctggccagat cgcatgcgcc
     4681 gccgctgccg ggcagccgcg tttcgatctg gtcaggaccg ccgatgtagc tcgtgttcaa
     4741 gttgccgaac cgatcgacct gcgcgccgcc gatgaagctc acgtcgacga ggccctgctg
     4801 cagcaggccc atcacgtcgc cggtgtcggc cagccacgtc gcgcgatgga gattcggcaa
     4861 gtcgcccatc gtgaagatcg gttcgggcgc gggcgcgtct cgcacgatgc ccagctcgaa
     4921 gacgccgacg gcgttgggtg cgtgtgcctg cttcgccagt tgaaagccga gcagcggcag
     4981 gcgcatgccg acgaagacca catcaccatc attgatttca cgcgcagcca ccgctaccat
     5041 caattcagcc gccgtgtagg ggcgaggctc tccctcgccc tcacgcacat cctcgccccc
     5101 aggttcgcct ttagtatcca tagtcaacgg cctccgccgg cgcatggtgt ttgagcctca
     5161 gcccggcgac tcgttcttcc cccagccgcg ccacataatc ctcgctgctg cgcaccccgt
     5221 ctacccagcg cgcctgccat tccacgaacg cttccggcgt ctggctctcc tcctggtaat
     5281 cgaggaacat ctgatgatcg cggttgtagt gacccggcac gggcgaggga tgcgcgcccc
     5341 acggcgcgtg ggcaacggcg ctgacgcgaa
//
LOCUS       DBNHXB010000011         3812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_673610, whole genome shotgun sequence.
ACCESSION   DBNHXB010000011 DBNHXB010000000
VERSION     DBNHXB010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_673610"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="AC162G_00355"
     CDS             <1..897
                     /locus_tag="AC162G_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5074296.1"
                     /translation="GQFAAPAGQAVIMFYQDPTLTIGPGIVKSILNQFMDGMAGAKIA
                     VSVALQEMGTLDSNRIGQIVQLYLAGSTTQTGDPAAALLDVRASGASQPATNPLLAIV
                     GPIMGGMMIFFAFYTGTATAQTILEEEEEQTLPRLFTTPTPQAVILTGKFLAVFLTVL
                     VQVVVLLVAARLIFRIEWGELPAVALVVAGTVIIAASFGIFFMSLLKNAKQGGAIFGG
                     VLTVTGMIGAISIFAQGSPAAQQLGSTVSLLVPQGWAVRGLIQAMNGLPVVDVLLTTL
                     VMLVWSAVFFTVGVLRFNRRYA"
     gene            907..2106
                     /locus_tag="AC162G_00360"
     CDS             907..2106
                     /locus_tag="AC162G_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5074297.1"
                     /translation="MIRIIDIALKDLMQLLRERETFLFLLILPIIFTLLFGFAFGGFG
                     GGESDPRLPVGYLDQDDSPLSRELHGLLDASEVIRLDEDDTRAPTDLEKLVADEKLAA
                     AIVVPAGYGEATLAGAPTRLILFADTASPAGRSVQNEALTAVNRLASAVRTANIVTQV
                     AGDRAAFDSALQKTIAAWERPPIAVAETTSTAIEQKSNSALSLAHTSPGMMVQFAIAG
                     LLTAAQIIVAERKSRALQRLLTTATARVHILLGHYLAMLALILGQFVILIAFGQIVLK
                     VDYLREPGATTLMAVTTAMCVAALGLLIGTLARSEEQAIIFSLVPMFVLAALGGAWVP
                     LEVTGAAFQAIGHVSPVAWAMDGFKNVVSRGLGLESVLLPALALLGYAVLFFAVAVWR
                     FHSSEEQ"
     gene            2116..>3812
                     /locus_tag="AC162G_00365"
     CDS             2116..>3812
                     /locus_tag="AC162G_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5074298.1"
                     /translation="MTITNLAPASNRPERQFTLAQIVGLWAVVTVPVAFLAWFVVPAV
                     VDRVNFPAVIIYWLIVMVGMAWQTAVSLWVISRQEGNLRWETIRRFIWLNKPRDPKTG
                     VPRLRSFWRMLPRWPIVVVVLGIGVLMPVWLLFMIRFGVFDYYDIPVLLWPASANITE
                     LASPEFAGQWWLVIVVFLGWAWSALLAEELLFRSVLLPRMGGVFGKRDWSVNALLYAL
                     YHLFQYWMIPFRLIEGLIVARQTRRFSSYWMAVAIRSVEGLAVLALVVLGVTSQPLTT
                     SPVPDQMPYISRRPAPSVLYRGPIAAIPAYNPTSGSPYQVDLRGADLSALDLRNTIND
                     LAYADFDTRTVWPAADQMPAGYDPTQILELGKSPGLGIHILHAQGITGRGVGVAIIDQ
                     PLLTEHKEYADRLRWYEEIPGIFESPAQMHGLAVASLAMGQTVGVAPEADLYYIGGAG
                     TGAQSLVLYSHDYAQAIRRIVQINEQLPAERKIRVISISSGWLPWIAGYHDVVMAVRE
                     AEAAGMLVVNVGTGTALPNNVWLSGLGRPPMADPGTFESYEPGIFWAEEFYTHGIQH"
BASE COUNT          647 a         1142 c         1202 g          821 t
ORIGIN      
        1 gggcagttcg ccgcgcccgc cggccaggcc gtgatcatgt tctatcaaga cccgacgctg
       61 accatcggcc ccggcatcgt caagtccatc ctgaaccagt tcatggacgg catggcgggt
      121 gcgaagatcg ccgtgagcgt cgcgctgcaa gagatgggaa cgcttgacag caaccggatc
      181 gggcagatcg tccagttgta cctggccggc tcgacgacgc agacgggcga tccggccgcc
      241 gcgctgctcg acgtgcgcgc ctcgggcgca tcgcagccgg cgaccaaccc gcttctcgcc
      301 atcgtcgggc cgattatggg cggcatgatg atcttcttcg ctttctacac cggcacggcc
      361 accgcccaga ccatcttgga ggaggaggaa gagcaaacac tgccgcgcct gttcaccacg
      421 cccacgccgc aggcggttat tctcaccggc aagttcctgg ccgtctttct gaccgtgctg
      481 gtgcaggtcg tcgtgctgct cgtcgccgcg cggctgatct tccgcatcga gtggggagag
      541 ctgcctgcgg tcgcgctggt ggtggccggc accgtgatca tcgccgcgtc gtttggcatc
      601 ttcttcatgt cactgctcaa gaacgccaag cagggcggtg ccatcttcgg cggcgtgctg
      661 acggtgacgg gcatgattgg ggcgatcagc atcttcgcgc aaggctcgcc ggcggcgcag
      721 cagctcggca gcaccgtctc gctgctggtg ccgcagggct gggcggtgcg cggcctgatt
      781 caggcgatga acgggctgcc ggtcgtggat gtgctgctga ccacgctggt catgctcgtc
      841 tggagcgcgg tcttcttcac ggttggcgtc ttgcgtttca atcgacggta tgcgtaggag
      901 agagccatga ttcgcatcat cgacatcgcc ctgaaagacc tgatgcaatt gctgcgcgag
      961 cgcgagacct ttctgtttct gctcatcctg ccgataattt tcaccctgct gttcggcttc
     1021 gccttcggcg gattcggggg cggcgaatcg gacccgcgcc tgccggtcgg ctacctggat
     1081 caggatgaca gtcccctcag ccgagaactc cacggcctgc tggatgcgtc ggaagtcatc
     1141 cgtctggatg aagacgacac gcgcgctccc accgatctgg agaagctggt agccgatgag
     1201 aagctggctg ccgccatcgt cgtcccggcc ggatacggcg aggcgaccct ggccggcgca
     1261 ccgacgcggc tgattctgtt cgccgatacg gccagcccgg ccggcaggag cgttcaaaac
     1321 gaggcgctga ccgctgtcaa tcgactggcg agcgccgtgc gcacggcgaa catcgtgaca
     1381 caggtcgccg gtgaccgggc ggcgttcgat tccgccctgc aaaagacgat tgccgcttgg
     1441 gagcggccac cgattgccgt ggccgagaca accagcaccg ccatcgaaca gaaaagcaac
     1501 agtgccctgt cgctggcgca tacctcgccc ggaatgatgg tgcagttcgc cattgccggg
     1561 ttgttgaccg ccgcgcagat catcgtcgcc gagcgcaagt cgcgcgcctt gcagcgcctg
     1621 ctgaccacgg ccacggcgcg cgtccacatc ctgctgggtc attacctcgc catgctcgcg
     1681 ctcatcctcg gccagttcgt gattctgatc gcctttggcc agatcgttct gaaggtggac
     1741 tatctgcgcg agccgggtgc gaccactttg atggccgtca cgacggcgat gtgcgtcgcc
     1801 gcgctggggc tgctcatcgg cacgctggcg aggagcgaag agcaggccat catcttctcg
     1861 ctggtcccga tgttcgtcct ggccgcgctg ggcggcgcgt gggtgccgct cgaagtcaca
     1921 ggcgcagcct tccaggccat cggccacgtc tcgcccgtcg cctgggcgat ggacggcttc
     1981 aagaacgtag tctcgcgagg gttagggctg gaatcggtgc tgctgccggc gttggcactg
     2041 ctgggctatg ccgtgctgtt cttcgcggtt gcggtgtggc gtttccattc gagcgaagag
     2101 cagtgatact gaaagatgac cataaccaat ctcgcgccgg ccagtaaccg gccagaaaga
     2161 cagttcacac ttgcgcagat agtcgggctt tgggcggtcg tgaccgtgcc agtagcattc
     2221 ctggcctggt ttgtcgtccc ggctgtggtg gaccgggtta acttcccagc cgtgatcatc
     2281 tactggctta tcgtgatggt aggcatggcc tggcagacgg cagtttcgct ttgggttatc
     2341 tcacgtcaag aaggcaacct acgctgggaa accattcgcc gctttatctg gctgaacaag
     2401 ccacgtgatc ccaaaacggg cgtgccacgc ttgcgctcgt tttggcgaat gctgcctcgc
     2461 tggccgattg tggtcgttgt tttgggtatc ggtgttctca tgccggtttg gttgctcttc
     2521 atgataagat tcggtgtctt tgattactac gacattcctg ttttgctatg gcctgcatct
     2581 gccaacatca ccgagttggc cagcccggag tttgccggtc agtggtggtt ggtaatcgtg
     2641 gtgttcctcg gctgggcctg gagtgcgctt ttggcagagg aactcctgtt tcggagtgtg
     2701 ctgcttccca ggatgggcgg tgtgtttggc aaaagggatt ggagcgtcaa tgcgctgttg
     2761 tatgcgcttt accatctgtt tcaatactgg atgattccgt tccggctgat cgaggggtta
     2821 atcgtcgctc gacagacccg gcgtttctcc agctactgga tggctgttgc cattcgaagt
     2881 gtagaagggc ttgccgtgtt ggcactcgtg gtgttgggcg tcacctctca accgttgacg
     2941 acatcccccg tccccgacca gatgccctac atcagccgga ggccagctcc atcagtcctc
     3001 tacagggggc ctatcgcggc gatccccgct tacaatccaa ccagtggctc accttaccag
     3061 gttgacctgc gcggagcaga tctatccgcg ctcgatctac ggaacaccat caacgatttg
     3121 gcttatgctg actttgatac ccggacggtt tggcctgctg cggatcagat gccggcgggc
     3181 tatgatccaa ctcaaatcct tgagttaggc aagagccccg ggttgggcat tcacatcctc
     3241 catgcccagg gcatcaccgg tcgtggtgta ggcgtggcca tcattgacca accactgctc
     3301 accgagcaca aggagtacgc cgatcgcttg cggtggtatg aagagatccc cggcatcttt
     3361 gaatccccag cccaaatgca cggcctagcg gtcgcttcgc ttgccatggg tcagacggtc
     3421 ggcgtagcgc ccgaggccga tttgtattac atcggcgggg cgggaactgg agcgcagtca
     3481 ctcgttctct atagtcacga ttacgctcag gcgatccggc gcatcgtgca gatcaatgaa
     3541 cagttgccgg ccgagcgcaa gatccgtgtg atttccatct cgagtggatg gctgccctgg
     3601 attgcaggat accacgatgt cgtgatggca gttcgggagg cagaagcggc agggatgctg
     3661 gtggtcaatg tcggaacggg aaccgcgttg ccgaacaatg tttggctgag tgggttaggg
     3721 cgtccgccca tggccgaccc gggtaccttc gagtcttacg agccaggcat attttgggct
     3781 gaagagtttt acacacacgg catccagcac ag
//
LOCUS       DBNHXB010000012        18800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1009105, whole genome shotgun sequence.
ACCESSION   DBNHXB010000012 DBNHXB010000000
VERSION     DBNHXB010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 18800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18800
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1009105"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="AC162G_00370"
     CDS             <1..601
                     /locus_tag="AC162G_00370"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR5074299.1"
                     /translation="ERELAEQFGVSRTAVREAVKALRQRGLVEVQPGRGTFLTDGTSQ
                     AVRHSLDLLMKIGGAVGSANLVEVREIIEPEIAALAAARASEAHLAALREAVTTMDAA
                     LHDPDLFIEADLDFHLALAEATQNALILTLIDSIVDVLREQRARIFRVAGGPQRGQFH
                     HKRILETIVGHDPDAARAAMRAHLRQVREDSEASVRLSG"
     gene            626..1771
                     /locus_tag="AC162G_00375"
     CDS             626..1771
                     /locus_tag="AC162G_00375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF028222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR5074300.1"
                     /translation="MPTLGFVGLGVMGSTSRNTQHASLRGSAGMLAVVFPRPDEFTLA
                     DVPEPEAGLGQVLVRVKSTTVCATDFKIFHGQFPGVTFPHTPGHEWGGEIVAIGAGVS
                     GLRVGDNVGVEAHVGCGACPRCSEGLYNLCENYGRPETGHAHIGFTVPGGLAELCAIP
                     ARAAHVLPESLDSDHGAFTDTLGIVLWTFERAGGVQAGEAVAVVGPGALGLLAVQLAR
                     ALGAGQVIAIGAAEDTDRLALALRLGADRALNVAEAANPAQVVRDLTGGRGADLVIEF
                     AGSAEAGRQSLEMARRGGRVVLGGSTAPGRRLDVDLSTIVRGHLSVFGSVGNPRGISR
                     RANALMQKGFVDVRPLITHHLPLSEFARAWEIFGDRTEGAIRVMLHP"
     gene            1768..2802
                     /locus_tag="AC162G_00380"
     CDS             1768..2802
                     /locus_tag="AC162G_00380"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR5074301.1"
                     /translation="MRALVKRERGPGHLELRDVPVPQITTDEVLIRVRACGICGSDLK
                     IQDDHHPYTPPVVIGHEFAGEIAEVGSGVTGWAAGDRVVSEQHTRACGRCRQCLTGNA
                     FACASKRAPGYFVDGAFAEFIKVPAWLLHRIPDNLSFVEASFSEPSAVAAHGLLDRTG
                     VVPEDVVLVLGCGPIGLVAARMAQAAGASKVIITGVERDEKARLPKARELGIDCVVNV
                     EQTDLAALVADLTGGEGADVVVELSGAPSAIAQSFRLARRLGRVGIIGQPPSDDITIP
                     YREALFRALTVSFSYSSKYTSWERALSLFGRGAIRPAQFVTHVLPLAEWERGFELSRS
                     GEAVKVVLEP"
     gene            2832..3899
                     /locus_tag="AC162G_00385"
     CDS             2832..3899
                     /locus_tag="AC162G_00385"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF028222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR5074302.1"
                     /translation="MDIPKTMKAAVLFDYGDLRVTQKPVPDLTTGEALVKVDSVAICG
                     SDPAIIAKGWPNHPPLGEFTPGHEFTGTVVDVAPDVTELKVGDRIAVEPHKGCGRCVN
                     CLRGLYTTCLNYGKPEKGHRHYGFSSNGGYAEYAACHVNTLHKLPDSISFEEGALLTT
                     AGTVLYGYERIGWVKPGETVVVTGPGAIGLISVQAAKILGAGRVILTGTREDRLTVGR
                     QLGADVTLNIKEVNAREAIMELTDGLGADLVVECSGQPGPAAEALEIVCKNGRICYNG
                     IYHEPVTLHLNKIVQWNLLIAGPKAEGMWNLERAIPLMADGRLKMKPLITHAYCLDDI
                     NRAFDTFTGRAGGAIKVIVKP"
     gene            3911..4879
                     /gene="iolN"
                     /locus_tag="AC162G_00390"
     CDS             3911..4879
                     /gene="iolN"
                     /locus_tag="AC162G_00390"
                     /EC_number="3.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-scyllo-inosose hydrolase"
                     /protein_id="HIR5074303.1"
                     /translation="MGNWQIPAQGGHLDLPDGIYFQNMTMKEVEARLKKNDLIIIPVG
                     STEAHGPHACYGEDTFLVTRMAEAVAQRTGCTVSQPVWFGSHPYHHIGMPGTVPVDED
                     TFTAYLSSIIAGFWNTGFRKQILLNGHGQEYVIPTAIHRFAKRYQVPSILINLNWYYA
                     IQPHIKDKAHGGPFETPFIHADECETSYSLALFPEMIHMEDAVDTEFHGFLPPGHVDS
                     AANAYQRAIPWYAHAGLGTIEIVGNPEGVVGKATLADVEKAGAGVEALLDYMVKLQDD
                     IMERFPPGKLPDAPLVTQRFTQAEVDELLKGPLNGGKHLYTVAWPP"
     gene            5029..5154
                     /locus_tag="AC162G_00395"
     CDS             5029..5154
                     /locus_tag="AC162G_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074304.1"
                     /translation="MAVGGNLVHPAAIAAAEFWRLTDTARQLFTEVQKGRGAKSP"
     gene            5154..6089
                     /locus_tag="AC162G_00400"
     CDS             5154..6089
                     /locus_tag="AC162G_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5074305.1"
                     /translation="MRVAFTDFVFENREAIRLDGCEFSLHGPLRTADEIVSAAAGADV
                     LCMRDQFGRVTREVLERLPDLKLIVTRSAGYDHIDLVEAARRGIPVCNVPDYGAHMIA
                     ELAFGLLLAVARNIVLGHERYRNERRFSDEGLKGIELRGKTLGVIGTGRIGLHCIRIA
                     SGFGMKVIAHDVAENEAAAQEMGFAYAPLDDVLVESDAVTLHVGLNETTHHLINAERL
                     AHMKPGAILINTSRGAVVDTRALIALLRSGHLGGAGLDVLEEERETYHDFSGLNVVVT
                     PHLGWYTDGAVHRILAITLENIAAVMRGKPINRVA"
     gene            6095..7084
                     /locus_tag="AC162G_00405"
     CDS             6095..7084
                     /locus_tag="AC162G_00405"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5074306.1"
                     /translation="MIIDTHAHIIVPEILRETAPEEAWRPRVFCESGKQVVDYAGKQI
                     KSAVREFVRIESILEAQMAAGIDRVLLCPWASLVRYDVEPDEGLRTSRIQNEALARLA
                     QAHPDRVSALGTVPLQDAALAARELESLMQEPGLHGVEVAASVRDAYLGDDRFRPFWA
                     AAEATGALVFIHPTTRGFSMPVFNDYYLWNTVGNPLETTITAAQMIMSGVMEAHPGLK
                     VLLAHGGGALLSLRGRLRHAHTFQPQAKSRLRESPEQSLKRFYFDTVTHDVDVLRGLV
                     DYVGVDRVLLGSDYPFDMGDERPAEIVRGLGLPAENEAKILGGNAARLLNPEV"
     gene            7362..8894
                     /locus_tag="AC162G_00410"
     CDS             7362..8894
                     /locus_tag="AC162G_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014683.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HIR5074307.1"
                     /translation="MALWLVALIPIAVVLVGMVGLRWSGARAGTVSWVVALGLAVVFF
                     GAGPSLLANAQLKSILLSLYVLYIIWMALLFYHVVNEAGVIKTLAAKLPGLTADKGLQ
                     VLVLAWAFGSFLQGVSGFGVPTAVVAPLLVGVGLGAEVAVVAAGLGHAWAVTFGSLAS
                     SFLALMAATGRGGPELASESAIMLGLACFACGVAVLWAVGGARMAAGHWWQVLVVGSV
                     MSVTQYAVAQLGVYTLAAAAAGLAGLGATVLLARLRRSTSDPPETPPPLSLGWALFPY
                     IALIVIVGSANLVGPLSDFLDGVMIRIRFPELTTALGYTTPAETGRTISVFGHAGALL
                     LYTSLISYAVFRARGNYSNGAARRIVRSTVKGSINSTIGILTMVAMAVTMEHAGMTRA
                     LAEGLATAAGSTYPAIAAFIGALGAFMTGSNTNSNVVFASLQDQTAQLLGYAPVVILA
                     AQTTGGAIGGAFAPAKVILGCSTVGLGGREGPVVRATLIYGLVILIIVGVMAFAWVGV
                     LS"
     gene            8894..9892
                     /locus_tag="AC162G_00415"
     CDS             8894..9892
                     /locus_tag="AC162G_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0003714 - transcription corepressor
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_function: GO:0140297 - DNA-binding transcription factor
                     binding [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HIR5074308.1"
                     /translation="MGLRSRIAVLGTRFGPPTIEAEELSDFDVEFVLGAAATADALVQ
                     DAGDAVAILAGSGPRFTREVLNRLPACRAIVRYGVGTETVDLQAATQAGILVANVPDY
                     CTEEVATHTLALILACERKLLQAQRATAGGQWQIAGVRPLFSTEDQVLGIVGFGRIGQ
                     AVARKAQPFGFEILVFDPIVTAGMAAERGVTRVDLAALLSRSDVVSLNAPLIDDTYHL
                     INAATIEHMKPTAFVVNTSRGGLIDEAALLDALNRGRLAGAALDVFEIEPVPGDHPLL
                     HSERCLVTPHMAWYTEQASERMRRLASREVARVLRGESPHHLVNPDVVRILADRKD"
     gene            9938..10702
                     /locus_tag="AC162G_00420"
     CDS             9938..10702
                     /locus_tag="AC162G_00420"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR5074309.1"
                     /translation="MSQPEAAALLARSPIAVIPTGSVEQHGPHLPFGTDYFAAWIIAR
                     RMARDLDALLLPFTPLGVTPIHMSFVGTISISPESYMGLLTDVCESIIQHGAQRLVIV
                     NWHELNKPFIDQVASRLQLKHAVRFVLVQAHFVALGLFGQEVGLTHGGLLEALPVLAY
                     DPSLVKLERATNPSPRDTASRLDKLRRGKEVYTVVKDVREMYPTGWYGTLEGATVEKA
                     HAFLEAVSAHCTQQVREALETLATSKTLTPHSNSPL"
     gene            10724..11869
                     /locus_tag="AC162G_00425"
     CDS             10724..11869
                     /locus_tag="AC162G_00425"
                     /inference="COORDINATES: protein motif:HMM:NF019912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR5074310.1"
                     /translation="MLSSSNAAEAWNVRLIGHSDLGGYGDGMQLMLKDGYLHVGHLKS
                     MGTSILDVSDPASPRVVKQLKNPPGTHTHKVQIGGDVLLINVEQHGSGVPERVGFQIM
                     DISNPVEPRELGFFATSGRGVHRMWYTGGDTVYLSATLDGYSDRILMIVDISNPTQPR
                     EVSRWWLPGMWRAGGEQPTWSPDLRVGAHHPIVVGDRAYLGFWDGGMCIMDLSDLANP
                     RLISRVSWAPEQGGHTHTALPLPDRNLLVVTDEATEPECQGPPTRVRLFDISDETQPR
                     LVSLFPEPQGDFCRRGLRFGPHNLHENRPGSLVSDQVMYVTYCNAGLRVVDISRPETP
                     KEIAYYIPRLPEGQTAIQTNDVFVATNGLIYISDRVLGGVDILELTV"
     gene            12032..13066
                     /locus_tag="AC162G_00430"
     CDS             12032..13066
                     /locus_tag="AC162G_00430"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5074311.1"
                     /translation="MSYEPVRVACVGIGRWSDVIARAVGRSDKLKVASCFTRSADKRA
                     AFAQKFGCDQAATYEELLADDRVEAVLVTTPNPMHLDTIAQAAAAGKHVWVEKPIAHS
                     MDHTKRIGEAVSKAGVTFSVGHSARLLGGSRKMKELVDQGDLGKVVLVEADYSNERAL
                     ELTPDKWRYYASNSPGGPLVQLLVHHFDTVQYILGPISEVQAYKRRLHTTAEVDDVAV
                     VIAQFDKGYLGTFGGSWSSPGAYSIDIYGTDANLYHTLDFAYWTALDVDQHCTLVREA
                     RGAKQRVPVELPTTDMFREELENFADAIRNGSRPEVGWEESARAMAVVEAAIRSSDIR
                     RPVSIAEVMA"
     gene            13110..13901
                     /locus_tag="AC162G_00435"
     CDS             13110..13901
                     /locus_tag="AC162G_00435"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5074312.1"
                     /translation="MLEVMAEHLTVEYELIRAKQTLTAIWDINLSVRPREFLVIVGPS
                     GCGKTTFINAVVGLVRPSRGRVLVGGKLVKSPGPDRAMVFQDYGLMPWRTAVDNVKFG
                     LELQARAQGSVDKIAYYINLVGLRGFEKNLPHELSGGMRQRVGLARALVTDPQVLLLD
                     EPFAALDAMTREFMQAEFERILAQTKQTVILITHSIDEAITMGDRIVVMTAGPGRIKA
                     ILTNNIPRPRWEGDLHLHPRYPELREEIWNLLKAEQHDQLSNPAH"
     gene            13924..14778
                     /locus_tag="AC162G_00440"
     CDS             13924..14778
                     /locus_tag="AC162G_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5074313.1"
                     /translation="MTATLNAPVESQRSVPRLRPIERPLGFEGFWARYGNIVGTLINV
                     STFFIIWELAARAQVLPRLLLPPVSEIIGEFLELAQSGQLFYHLTFTVSTLTTGFLIA
                     TAAGVPIGLLLGASRKMERILGPYMWALFATPRLALLPLITVAMGFGFAPKVLLIFLA
                     SFFVIAIDTWAGVKTVEPSLIRAARVFGANRAQIFAKVVVPYTLPFIVNGLRVGVTRA
                     IITALVAEMIASSRGLGYVVVRSMDTFNTARMYSMVLLLVFTSLAIVSLLRWLEAKVA
                     PWREVGSI"
     gene            14928..16010
                     /locus_tag="AC162G_00445"
     CDS             14928..16010
                     /locus_tag="AC162G_00445"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5074314.1"
                     /translation="MSARHNNRRGLSRREFLGLSAGLGAGAGSAWLLGACAPAAPAAT
                     PTAAANLMAETKHLINGTYNPNWANNVLVELADAQGWFKEEGIESREIVIIDQGQIIP
                     ALIGGSLMTAQQDTDAIAAANLAGEPVFFISTYRDKEPWIFGVAKGINSAEDLKGKQV
                     SGGQLGSRNENNGKEMLRRLGLDPEKDVEWVPVGGGSDGRVQGVIGGVIAGTVMQDRH
                     MKLIEDAGGKILYNERESVPQDCYCAHRSWLDKNERTAIGFIKATFKAREYVLDLSRK
                     DEIIEFMKSRGYEFPEAFVLGYEDLLAIMSPTGTFTVDTMVKLIQDAVKAGTLEKEVD
                     WHTFVDLKYQNQAFKELGRDDLVMNV"
     gene            16096..17373
                     /locus_tag="AC162G_00450"
     CDS             16096..17373
                     /locus_tag="AC162G_00450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR5074315.1"
                     /translation="MLNDWTEVRYDLDKSANFVEICNSPWYGDAIYDRFSDAEMARRH
                     TLAREMMAREGVAALILTGSPNIYSMGGAVTWASGLIDERGMCQYMLLPRAGEPMLIY
                     PHAGCHIEAARRMVSVQDVRGSQGGHFGKVLAERLLALGLGESRIGIVACDRNGPEYM
                     GISCYRELTEHLPRAEFVFLPGLLHELTYLKSTEEIGAMARAGELAVEAMDAVAAAAR
                     PGAREYQLESAGTFAIMNGGGRVHLMMIGTTSMHDPRLVFPNPRPSHRILQEGDIVVP
                     EIVAMYKGYSAKIGHPVTIGPPTQEVDAFFKEVTLGGFHALREQLMPGKTLEDVRQAG
                     AYFRRHGAQARPLMVHGLDLITATPYISVDQVKARPAEMTIVPGMTFAIEITPINPDG
                     TFGMFMGRTYAITEDGQRDLTPYPMDEIIVAPA"
     gene            17469..18233
                     /locus_tag="AC162G_00455"
     CDS             17469..18233
                     /locus_tag="AC162G_00455"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR5074316.1"
                     /translation="MKEEVRLERLSQPEAEARLKASRTAVLAPGSIEQHGPHLPLGTD
                     AFAADALAEEVARKLGSVLVPMPLVGVSPYHLSWAGSLSFKPQTMMAVVEDICECLAA
                     HGVERILLVNWHEGNTPALRLAGDNVQRKFNLRVVVAESHLITHQLFGERAQLTHAGN
                     METAAVLMVDPSLVDLSKATNPTPHDEGDEGHRIFRQRDVYPIMRDFHEVARTGWYGK
                     PEEATPELAAEIRQKVSDTIVERARIEFERERHIPH"
     gene            18246..>18800
                     /locus_tag="AC162G_00460"
     CDS             18246..>18800
                     /locus_tag="AC162G_00460"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR5074317.1"
                     /translation="MNSIPTTMRAAVTRDWNDIRLEQVAIPELEPGEVLVRVGACGIC
                     GTDLKIVSGVYKGSWPPSLPFIQGHEWGGAVAALGEGVTSLQVGDRVASENHKGCGAC
                     AMCRRGRYNLCEVARTKGKAYKLYGHSAQGAFAEYAARPAGLLHKLPDSVSFEESTIV
                     NQGALGLHAIRRCRIDPGDTVAVIG"
BASE COUNT         3354 a         5735 c         6342 g         3369 t
ORIGIN      
        1 cgagcgtgag ctggccgaac agttcggcgt cagccggacg gccgtgcgcg aggcggtcaa
       61 ggcgcttcgc cagcgcggcc tggtcgaggt gcagcccggc cgcgggacct ttctcaccga
      121 cggcacgtcg caggcggtgc ggcattcgct ggacctgctg atgaagatcg ggggcgccgt
      181 cggctcggcc aacctggtgg aggtgcgcga aatcatcgaa cccgagatcg ccgccctggc
      241 cgccgcgcgc gcgagcgaag cgcacctggc ggcgctgcgc gaagcggtca cgacgatgga
      301 cgccgcgctg cacgacccgg acctgtttat cgaagccgac ctggactttc acctggcgct
      361 ggccgaagcg acgcagaatg cgctcattct cacgctgatc gattcgatcg tcgacgtgct
      421 gcgcgagcag cgcgcgcgca tcttccgtgt cgcgggcggc ccgcagcgcg gccagtttca
      481 ccacaagcgc atcctggaga ccatcgttgg gcacgatccc gacgcggccc gcgcagcgat
      541 gcgtgcgcat ctgcgccagg ttcgtgaaga cagtgaggcc tcggtccgcc tgagcggctg
      601 aggcctcgcg cttgggagga gcgagatgcc cactctcggc tttgtcggcc tgggtgtcat
      661 gggcagcact tcacgcaaca cgcaacacgc ttccctacga ggttcggcag gcatgctcgc
      721 cgtcgtgttt cccaggccgg atgaattcac tctcgccgac gtgcccgaac cggaagccgg
      781 gctgggtcag gtgctggtgc gagtcaagtc gaccaccgtc tgcgcgacgg acttcaagat
      841 ttttcacggt caattccccg gcgtgacgtt cccgcacacg cccggccacg agtggggtgg
      901 cgagattgtt gctattggcg caggcgtaag cgggctgcgt gtcggtgaca atgtgggcgt
      961 ggaggcccac gttgggtgcg gcgcgtgtcc gcgctgctcc gaggggctct acaacctgtg
     1021 cgagaactac ggtcgtccgg agaccggtca cgcgcacatc ggcttcaccg tcccaggtgg
     1081 gctggcggag ctgtgtgcca tcccggcgcg cgccgcgcac gtgttgccgg aaagcctcga
     1141 cagcgatcac ggcgcgttca ccgacacgct cggcatcgtg ctgtggacct ttgagcgcgc
     1201 gggtggcgtg caggcgggtg aagcggtagc cgtcgtgggg ccgggcgctt taggactgct
     1261 cgccgtgcag ctcgcgcgtg cgctcggcgc ggggcaggtg attgccatcg gagcagccga
     1321 agatacggat cgattggcgt tggcgctgag gttgggcgcg gatcgcgcgc tcaacgtggc
     1381 cgaggccgcc aatccggcgc aggtagtgcg cgacctgacc ggcggacgcg gcgccgatct
     1441 cgtcatcgag tttgccggta gcgccgaggc cggtcgacag tccctcgaaa tggcccgacg
     1501 cggcgggcgt gtggtcctcg gtgggtccac cgctcccggg cgacgcttgg acgtcgattt
     1561 gagcacgatt gtgcgaggtc acctttccgt gttcggctcg gtggggaatc cgcgcggcat
     1621 atccaggcgc gcgaacgctc tgatgcagaa aggattcgta gacgtgcgcc cgctgattac
     1681 gcatcacttg ccgttgagcg aatttgcgcg ggcgtgggag atctttggcg atcgcacaga
     1741 gggcgcgatc cgggtgatgt tgcatccatg agggcgctcg tcaagcgcga acgtggcccc
     1801 ggccacctgg aactgcgcga tgtgcctgtg ccgcagatca cgacggatga agtgttgatt
     1861 cgagtgcgcg cctgcggcat ctgcggaagc gacctgaaga ttcaagacga ccatcacccg
     1921 tacacgccgc cagtggtgat cggacacgag ttcgccgggg agatcgccga agttggctca
     1981 ggcgtgacgg gctgggctgc gggcgatcgc gtcgtatccg agcagcacac acgcgcctgc
     2041 gggcgctgtc gccagtgcct gaccgggaac gccttcgcct gcgcatccaa acgcgcgccc
     2101 ggctactttg tggatggtgc attcgctgag ttcatcaaag tcccggcctg gctgctgcat
     2161 cgcatcccgg acaatctgag tttcgtcgag gcctctttca gcgaaccttc ggcggtggcg
     2221 gcgcatgggc tcctcgatcg tacgggcgtt gtgccggagg acgtggtgct cgtcctgggt
     2281 tgcgggccga tcggcctggt ggccgccagg atggcgcagg cggcgggtgc gtcgaaagtg
     2341 atcatcaccg gcgtcgagcg cgacgaaaaa gcccgactgc ccaaggcgcg tgaactcggc
     2401 atcgattgcg tggtgaacgt ggagcagacc gacctcgcgg cgctggtggc ggacctaacc
     2461 ggcggggagg gggcggacgt ggtcgtcgaa ctctcgggcg cgccgtcggc gatcgcgcag
     2521 agtttccggt tggctcgccg cctgggtcgg gtgggcatca tcggtcagcc gccgtcggac
     2581 gatatcacca tcccgtatcg cgaggcgttg ttccgggcgc tcacggtctc gttcagttac
     2641 agttcgaagt acacgagctg ggagcgtgcc ctgtcgttgt tcgggcgtgg cgcgatccgc
     2701 ccggcccaat tcgtcacgca cgtcctgccg ctcgcggaat gggagcgtgg ctttgagctc
     2761 tcgcgcagcg gcgaggccgt gaaggtggtt cttgaaccgt agcgggggat actcaagaga
     2821 aaggaatgcg aatggatatc cccaaaacaa tgaaagccgc agtgttgttc gactacggtg
     2881 atttgcgggt gacacagaag ccggtgcctg atctgacgac gggtgaggca ctggtcaagg
     2941 tagattcagt ggcgatctgc ggcagcgacc cagccatcat tgccaaaggt tggccgaacc
     3001 atccgccgct gggtgaattc acccccggcc acgagttcac cgggacggtc gttgacgtgg
     3061 cgccggacgt cacggaattg aaggtgggcg atcggatcgc ggtggagccg cacaagggat
     3121 gtggccgctg cgtgaactgt ctgcgcggcc tgtacaccac ctgcttgaac tacggcaagc
     3181 cggagaaggg ccaccgacac tacggcttca gcagcaacgg cgggtacgcc gagtacgctg
     3241 cctgccacgt caacacgctc cacaaactgc cggacagcat ttcgttcgag gagggtgcgc
     3301 tgctcaccac cgccgggacg gtgctgtatg gctacgagcg catcggctgg gtgaagccgg
     3361 gcgagacggt ggtcgtcacc gggccgggag ccatcgggtt gatctcggtg caggctgcca
     3421 agatcctcgg cgcggggcgc gtgattctca ccggcacgcg cgaggatcgg ctgaccgtcg
     3481 ggcggcagtt gggcgcggac gtcacgctca acatcaagga agtgaacgcg cgtgaggcga
     3541 tcatggaact caccgatggt cttggcgccg acttggtggt ggaatgtagc ggccagccag
     3601 gaccggccgc cgaggcgctc gagatcgtct gcaagaacgg gcgcatttgt tacaacggca
     3661 tttatcacga gccggtgacg ctccacctca acaagatcgt ccagtggaac ttgctgatcg
     3721 ccggccccaa ggccgagggc atgtggaacc tcgagcgtgc gatcccgttg atggccgatg
     3781 gccgcctcaa gatgaaaccg cttattacgc acgcgtactg cctcgacgac atcaacaggg
     3841 ccttcgatac cttcaccggt cgtgccggcg gggccatcaa agtcatcgtc aagccgtaaa
     3901 ggagaccgac atgggcaact ggcaaatccc tgctcagggt ggacatctgg acttgccgga
     3961 cggcatctac tttcagaaca tgaccatgaa agaggtcgaa gcccggctga aaaagaacga
     4021 cctgatcatc attcctgtgg gcagcactga agcgcacggg ccacacgcct gctacggcga
     4081 agatactttc ctggtcacac gcatggcaga agcggtggcg cagaggactg gctgcaccgt
     4141 gtctcagcca gtgtggttcg gcagccatcc ctatcatcac atcggcatgc ccggtaccgt
     4201 gccggtggac gaggacacgt tcactgccta cttgagctcg atcatcgcgg ggttctggaa
     4261 caccggcttt cgcaagcaga tcctgctcaa cggtcacggg caggagtacg tcatccccac
     4321 ggccattcat cgcttcgcca aacgctatca ggtgccctcc atcctgatca acctgaattg
     4381 gtactatgct atccagccac acatcaagga caaggcgcac ggcggcccgt tcgagacgcc
     4441 attcatccac gccgacgagt gcgagacatc gtattcgctg gcgctgttcc cggaaatgat
     4501 ccacatggag gacgcggtgg acaccgaatt ccacggcttt ctgccgcccg gccacgtcga
     4561 ctcggcggcc aacgcgtatc agcgcgcgat tccgtggtac gcgcacgccg gcctggggac
     4621 gatcgagatc gtcggcaatc ccgaaggtgt cgtgggcaag gcgaccttgg cggacgtgga
     4681 gaaagccggt gctggcgtcg aggcgttgct cgactacatg gtgaaactgc aagacgacat
     4741 catggagcgc ttcccgcccg gcaaactgcc tgacgcgccg ctcgtcacac agcgcttcac
     4801 gcaggccgaa gtggacgaac tgctgaaggg tccgttgaac ggcggcaaac acctgtacac
     4861 agttgcctgg ccaccgtgaa gacctcccag gtctcaaaga cctgggaggt ctaggcgcgc
     4921 gtgtggcggg cttcctcacc ggttgccttt gccccacata gggttggttc ccaccggcgg
     4981 ggtgagcgcg gccaacgcag gcgactagat ccgcgcggcg cagtggcggt ggctgttggc
     5041 ggaaacctgg ttcatcctgc cgccatcgcg gcggcggagt tctggcggct gaccgacacc
     5101 gcgcgccagt tgttcaccga ggtgcagaag ggcaggggag cgaagtcccc gtgatgcgcg
     5161 tcgccttcac cgacttcgtc tttgagaacc gcgaggcgat tcgcctggat ggctgcgagt
     5221 tcagtctgca cggcccgctg cgcacggccg acgagatcgt cagcgccgcc gccggagccg
     5281 acgtgctgtg catgcgagat cagttcggac gcgtgacgcg cgaggtgctt gagcgcctgc
     5341 ccgatctcaa gttgatcgtc acgcgctcgg cgggatacga ccacatcgat ctggtcgagg
     5401 ctgcgcggcg cggtattccg gtgtgcaacg tgccggacta tggcgcgcat atgatcgccg
     5461 agctggcctt cgggctgctg ttggccgtgg cgcgcaacat cgtgctgggc cacgagcggt
     5521 atcggaatga gcggcgtttt tccgacgaag gtctgaaggg tatcgagttg cgcggcaaaa
     5581 cgctgggtgt gatcggcacg gggcgcatcg gtctgcactg catccgtatc gcgagcgggt
     5641 tcgggatgaa ggtcatcgca cacgacgtgg cagaaaacga ggcggcggcc caggaaatgg
     5701 gattcgcgta cgcgccgttg gacgatgtgt tggtcgaaag cgatgccgtc actcttcacg
     5761 tcggtctcaa cgagacgacg catcacctca tcaacgcgga gcggctggcg cacatgaagc
     5821 cgggcgcgat cctcatcaac acctcgcgcg gcgcggtagt ggacactcgg gcgctcatcg
     5881 cattgctccg ttcggggcat ttgggcggcg cgggactgga tgtgttggaa gaggagcgcg
     5941 agacgtacca cgacttctcc ggcctgaatg tggtcgttac gccgcacctc ggctggtaca
     6001 ccgacggggc ggtccatcgc atcctggcca ttacgctgga gaacatcgcc gctgtcatgc
     6061 gcggcaaacc catcaaccgg gtggcgtaga aaacatgatc atcgacacgc acgcccacat
     6121 tatcgttccc gaaatcctgc gcgaaaccgc gcccgaagaa gcctggcggc cgcgcgtatt
     6181 ttgcgaaagc ggcaagcagg tcgtcgacta tgccgggaag caaatcaagt cggctgtccg
     6241 cgagttcgtc cgcatcgagt cgatcctgga agcccagatg gccgccggca tcgatcgcgt
     6301 cttgttgtgc ccgtgggcgt cgttggtgcg ctacgacgtc gaaccggacg aagggctgcg
     6361 caccagccgc attcaaaacg aggcgctggc ccggctggca caggctcatc cggatcgggt
     6421 gagcgcgctg ggcaccgtgc cgttgcagga cgcggctctg gcggcgcgcg aacttgagag
     6481 cctgatgcag gaaccgggcc tgcacggcgt agaagtggcc gccagcgtgc gcgatgcgta
     6541 tctgggcgac gatcgtttcc gcccgttctg ggcggcggcc gaggcaacgg gggctttggt
     6601 gttcatccat cccaccacgc gagggttctc tatgcccgtg ttcaacgatt actacctgtg
     6661 gaataccgtc ggcaacccgc tggagacgac catcacggcc gcgcagatga tcatgtctgg
     6721 tgtgatggaa gcccatcccg gcctcaaggt gctgctggcg cacggtggcg gcgcgctgtt
     6781 gagcctgcgc ggtcggctgc ggcacgcgca taccttccag cctcaggcca aatcgcggct
     6841 gcgggaatca ccggaacagt cgctcaagcg cttctatttt gacaccgtga cgcacgatgt
     6901 cgatgtcttg cgcgggctgg tggactacgt cggcgtcgat cgcgtactgc tcggctcgga
     6961 ttatcctttc gatatgggcg acgaacggcc ggcggaaatc gtccgcggcc tcggattgcc
     7021 ggccgaaaac gaagccaaga tcctcggcgg caatgcggcc cgcttgctga acccggaggt
     7081 ctgacgatgg ccaaactctt cccgaagaac gagccgtgcg tgccttcact cgctacgccg
     7141 ctcgcgcgct ggacaaagta gccagtggta gggcgagtca tggaggattc tcgtgatgcc
     7201 gggggccatc gttgaattat cgagtgccgg cagcgccgac aaaggagatc cgtgcttgac
     7261 gcgaagatac gggctgccat cgacagtcgg gcaccgcgcc tcatcgtgca gagtctggac
     7321 gacaggtacc tgcaccatac ctcagcccga gaggtgggtt gatggctctt tggttggtcg
     7381 ctctcatacc cattgcagtc gtgctggtcg ggatggtcgg tctgcgttgg agcggtgccc
     7441 gggcgggcac tgtgagttgg gtggtcgcgc tgggcctcgc ggttgtcttc ttcggcgcgg
     7501 ggccaagcct gctggccaat gcccaactca aaagcatctt gttgtcgctg tatgtgctgt
     7561 acatcatctg gatggcactg ctgttctatc acgtggtgaa cgaagcaggt gtcatcaaga
     7621 cgctggccgc caagctgccc ggcctgacag ctgacaaggg tcttcaagta ctggtgctgg
     7681 cctgggcgtt tggctcgttc ctgcaaggcg tgtccgggtt tggcgtgccc acggcggtag
     7741 tggcgccgct cctggtggga gtcggcttag gggcggaggt ggcggtggtt gccgccgggc
     7801 tgggccatgc ctgggctgtc acgtttggat cactggcatc gtcgtttctg gcgctgatgg
     7861 cagcgaccgg acgtggcggg ccggaactcg cttcggagtc ggccatcatg ctcggcctgg
     7921 cctgttttgc ctgcggcgtg gccgtgttgt gggcagtcgg cggagcgcgc atggccgccg
     7981 gtcactggtg gcaagtgctc gtcgtcggaa gcgtgatgag cgtcactcaa tacgcggtgg
     8041 cccaactggg cgtctacaca ttggcggccg cggcggccgg cttggcgggc ctgggtgcga
     8101 ccgtgctgct ggccaggctg cgtcgatcca cgtcggatcc acccgagacg ccgcctccgc
     8161 tctcgctagg atgggcgctg ttcccttaca tcgcgctgat cgtcatcgtg ggcagcgcca
     8221 accttgtcgg gccgctgagc gactttctgg atggcgtgat gatccgcatc cgatttcctg
     8281 aactgacgac ggcgctcggc tacaccacgc cggccgagac cgggcgcaca atttcggtgt
     8341 tcggccacgc cggcgcgctg ctgctgtaca cgtcgctgat cagttatgcg gtgttccgcg
     8401 ccagaggcaa ctactcgaac ggcgcggctc gccgaattgt gcgatcgacc gtgaaggggt
     8461 cgatcaactc gaccatcggc atcctgacga tggtggctat ggccgtgacg atggagcacg
     8521 cgggaatgac gcgggcgctg gctgaggggc tggccacggc ggccggatcg acctacccgg
     8581 caatagccgc cttcatcggc gcgcttggcg cgtttatgac cggcagcaac acgaacagca
     8641 acgtcgtgtt cgcgagcctc caggatcaga ccgcccagct cctgggctac gcgccggtgg
     8701 tgatcctcgc cgcgcagacg acgggcgggg ccatcggcgg tgcgttcgcc ccagccaaag
     8761 tgattctagg atgcagcacg gtgggcttgg gtggccgaga aggccccgtc gtcagagcga
     8821 cgctgatcta tggattggtc atcctgatca tcgtaggcgt gatggcgttc gcctgggtag
     8881 gggtcttgag ctgatgggac tgcgctcacg catcgccgtg ctgggaacgc gtttcggtcc
     8941 gccgacgatt gaagctgaag aactgtcgga ctttgacgtc gaattcgtgc tgggtgccgc
     9001 ggcgacggcg gacgcgcttg tccaggacgc cggtgatgcc gtcgcgatcc tggccggctc
     9061 ggggccgaga ttcacgcgcg aggtgttgaa tcgcctgccg gcttgccggg ccattgtgcg
     9121 ctacggcgtg ggcaccgaaa ccgtcgacct tcaggccgcg acccaggcgg gcatcctggt
     9181 ggcgaacgtg ccggactatt gcaccgaaga agtggcgacg cacacgctcg ctttgatcct
     9241 ggcttgcgag cgcaaactgc tgcaagcgca gcgcgccacg gcaggcggac agtggcagat
     9301 cgccggcgtg cggccgctat tcagcacgga agatcaggtg ctgggcattg tgggcttcgg
     9361 caggattggc caggccgtgg cccgcaaggc acagccgttc gggttcgaga tactcgtctt
     9421 cgatccgatt gtcacggccg gcatggcggc cgagcgcggt gtgaccaggg tcgatttggc
     9481 ggcgctgttg tcacgctcgg atgttgtgtc tctgaatgcg cccttgatcg acgacactta
     9541 tcacctcatc aacgcggcga cgatcgagca catgaagccg actgcgtttg tggtcaacac
     9601 cagccgggga ggactgatcg acgaggctgc actgctggac gcgctgaacc gtgggcggct
     9661 ggctggtgcg gcgctcgacg tgttcgaaat cgagccggtg cccggtgatc atccgcttct
     9721 gcactcggag cgatgcctgg tgacgccgca catggcctgg tacaccgagc aggcttccga
     9781 gcgcatgcga cgcctggcga gccgcgaggt ggctcgtgtg ttgcggggcg agtcgcccca
     9841 ccatctggtg aatcccgatg tggtgaggat tctcgccgat cggaaggact gagagacaag
     9901 tgatatgggc acggaaggcg aacacgcgtt gatagacatg tcccaacccg aggcggcagc
     9961 gctactggcc cgcagtccca ttgccgtgat acccaccggc agcgtcgaac agcacgggcc
    10021 gcacctgccc ttcggcaccg actactttgc cgcctggatc atcgcccggc gcatggcgcg
    10081 cgatctagat gccttactgt tgcccttcac gccgctgggc gtgacaccga ttcacatgtc
    10141 gtttgtcggg accatcagta tcagcccaga gtcttacatg ggcctgctga ccgatgtatg
    10201 cgagagcatc atccagcacg gcgcgcagcg actggtgatc gtcaactggc acgagctgaa
    10261 taagccgttc atcgaccagg tggcgagccg gctgcaactg aaacatgcgg tgcgctttgt
    10321 gctggttcag gcgcacttcg tagcgctggg actcttcggc caggaggtgg gattgacgca
    10381 cggcggcctg ttggaagcgc tgccggtgct ggcgtacgac ccgtccctgg tgaaactcga
    10441 gcgcgccacg aatccgtcgc ccagagacac ggccagcagg ctggacaaac tgcgccgggg
    10501 taaagaagtg tacacggtcg tcaaggacgt gcgcgagatg tacccgaccg gctggtacgg
    10561 cacgctggag ggcgcgaccg tggagaaggc gcacgcgttt ctggaagcgg tgtcggccca
    10621 ctgcactcaa caggtgcgag aggcgcttga gacgttggcg acgtccaaga ctctaactcc
    10681 ccactctaac tccccactct aactcccagc ggaggtcaca ctcatgttga gttcgagcaa
    10741 cgcagccgaa gcctggaatg tccgtttgat cggacactcc gacctgggcg gttacgggga
    10801 tggtatgcag ttgatgctca aggacggcta tctgcacgtc gggcatctca agagcatggg
    10861 caccagcatc ctcgacgttt ccgatccggc gtcgccccgt gtcgtcaagc aactcaagaa
    10921 cccgcccggc acgcacacgc acaaggtgca gatcgggggc gacgtgctgc tgatcaatgt
    10981 cgaacaacac ggcagcgggg ttcccgagcg ggtcggcttc cagatcatgg acatctccaa
    11041 cccggtcgag cctcgtgagc taggcttctt cgcgaccagc ggcaggggcg tgcatcgcat
    11101 gtggtacacc ggcggcgaca ccgtgtatct ctcggcgacg ctcgacggct acagcgatcg
    11161 catcctgatg atcgtcgaca tttccaatcc gacgcaaccg cgcgaagtct cgcgctggtg
    11221 gctgccgggg atgtggaggg ccggcggcga acagcccacg tggtcgccgg atttgcgggt
    11281 cggcgcgcac cacccgatcg tggtggggga tcgagcctac ctgggcttct gggacggagg
    11341 catgtgcatc atggacctct ccgatctcgc caacccacgg ctgatctcgc gcgtgtcgtg
    11401 ggcgccggaa cagggcggcc acacgcatac cgcgctgcca ctcccggatc gcaatttgct
    11461 cgtcgtcacc gacgaggcca ccgagccgga gtgccagggg ccgccgacgc gcgtccgcct
    11521 gttcgacatc agcgacgaga cgcaaccgcg cttggtcagc ctgtttccgg aaccgcaagg
    11581 cgacttttgc cggcgtgggc tgcgctttgg cccgcacaac ctgcacgaaa atcggcccgg
    11641 ctcactggtc agcgatcagg tgatgtacgt gacctactgc aatgcgggac tgcgcgtggt
    11701 ggacatcagc cggcccgaga cacccaaaga gatcgcctat tacatcccgc gcctgccgga
    11761 gggtcagacg gcgatccaga ccaacgatgt gtttgtcgcc acgaacggcc tgatctacat
    11821 cagcgatcgc gtgctgggcg gcgtggatat cttggagctg acggtgtgat gcgcagggaa
    11881 gcgggcatat tcctggggca gatcaccatc atcaccgacg cccgccacga gcgtgtggcc
    11941 cgtcatccgc tcgagctggc ctaattactg atcactgatt actgttcact gttcactgat
    12001 cactgatcac tgatgaggga ggcgtattca aatgagttat gaacccgtgc gcgtcgcgtg
    12061 tgtcggtatc ggccgctggt cggacgtcat cgccagggcc gtgggccgca gcgacaagct
    12121 caaagtggcg agctgtttca cccgctccgc cgacaagcgc gccgccttcg cccagaagtt
    12181 cgggtgcgat caggcggcga cctacgagga actgctggcc gacgatcggg tcgaggcggt
    12241 gctggtcacc acccccaacc cgatgcattt ggacacgatc gcgcaggcgg ccgccgccgg
    12301 gaaacacgtg tgggtcgaga agcccatcgc ccacagcatg gatcatacca agcgcatcgg
    12361 cgaagcggtg agcaaggcgg gcgtgacgtt ttcggtgggg cacagtgccc gcttgctggg
    12421 cggcagccgc aagatgaagg aactggtgga ccaaggcgat ctgggcaagg tcgtgctggt
    12481 ggaggccgac tattccaacg agcgggcgct ggaattgacg ccggacaagt ggcgctacta
    12541 cgcatcgaac tcgcccggag gtccgctcgt ccaattgctc gtccatcact tcgatacggt
    12601 tcaatacatc ctcggcccga tcagcgaggt gcaggcctac aagcgccgcc tgcacaccac
    12661 ggccgaggtg gacgacgtag ccgtggtgat cgcccagttc gacaagggtt acctgggcac
    12721 tttcggcggc agctggtcgt cgccgggcgc gtattccatc gatatctatg gcaccgatgc
    12781 caacctgtat cacacgctgg actttgccta ttggacggcg ctggatgtcg accagcactg
    12841 cacattggtc cgtgaagctc gcggcgcgaa gcagcgcgtg ccggtcgagc tcccgaccac
    12901 tgacatgttc cgcgaggaac tggagaactt tgcagatgcg atccgcaacg gcagccggcc
    12961 cgaggtcggc tgggaagagt cggcccgcgc catggccgtg gtcgaggccg ccatccgctc
    13021 gtcggacatc cggcggccag tgtctatcgc cgaagtgatg gcctgacgcg gggcaggccc
    13081 aagcgaaaca gtagtgctgg gaggacgaaa tgctggaagt catggccgaa cacctcaccg
    13141 tcgagtacga gctgatccgc gcgaagcaga cgctgacggc catctgggac atcaacctgt
    13201 ccgtcaggcc ccgcgagttt ctggtgatcg tcggaccgag cggctgcggc aaaacgacct
    13261 tcatcaacgc ggtggtcggt ctggtgcgac ccagccgagg acgggtattg gtcggcggca
    13321 agctggtcaa gagccccggt ccggatcgtg cgatggtgtt tcaggattat ggtctgatgc
    13381 cctggcggac ggccgtcgac aacgtgaaat tcgggttgga gctgcaggcc cgagcgcagg
    13441 gctcagtcga caaaatcgct tactacatta acctggtcgg gctgcgaggg tttgagaaga
    13501 atctgccgca tgaactgtcg ggcggcatgc gccagcgcgt gggcctggcc cgtgccttgg
    13561 tcaccgatcc ccaggtgctt ctgctggacg agcctttcgc cgctctcgac gccatgactc
    13621 gcgagttcat gcaggccgaa ttcgagcgca tcctggcgca gaccaagcag acggtgatcc
    13681 tgatcacgca ctcgatcgac gaggcgatca cgatgggcga tcgtatcgtg gtgatgacgg
    13741 ccggcccggg ccgcatcaag gccatcttga ccaacaatat ccctcgcccg cgctgggagg
    13801 gcgatctgca ccttcatccg cgctaccccg agctgcgtga ggagatctgg aatctgctca
    13861 aggcagaaca gcatgatcaa ctgagcaacc cggctcacta gactccggcc aggaggatgt
    13921 gccatgacag ccacgctgaa tgcgcctgtc gagtcccaac ggtcggtgcc gcgcttgcgg
    13981 cccatcgagc gcccgctggg atttgaagga ttctgggcgc gctacggcaa catcgtcggg
    14041 accttaatca acgtctcaac ctttttcatc atctgggagc tggccgcgcg cgcccaggtg
    14101 ttgccccgtc tgctgctgcc cccggtttcc gagatcatcg gcgagttcct cgaactggcg
    14161 cagagcggcc aacttttcta ccacctcacc tttacagtct caaccctgac gacgggcttc
    14221 ctgatcgcga ccgccgccgg ggttcctatc ggactgctgc tgggcgcctc cagaaagatg
    14281 gagcgcatcc tggggccgta catgtgggcg ctgtttgcga cgcctcgcct ggcgctgctg
    14341 ccgctgatca cggtggcgat gggcttcgga tttgcgccca aagtgctgct gatcttcctg
    14401 gcctcgttct tcgtcatcgc gatcgatacc tgggccgggg tgaagacggt cgagccgagc
    14461 ctgatccgcg cggcgcgcgt gttcggcgcg aatcgtgccc agatcttcgc gaaagtggtc
    14521 gtgccctaca ccctgccgtt catcgtcaac gggctgcgcg tcggcgtcac gcgtgccatc
    14581 atcacggccc tggtggcgga aatgatcgcc agctcgcgcg gcctgggcta cgttgtggtc
    14641 cgctcgatgg acactttcaa caccgcccgg atgtattcga tggtgctgct gctggtcttc
    14701 acctcgctgg ctatcgtgtc gctcctgcgc tggctggagg cgaaggtggc gccgtggcgg
    14761 gaagtggggt ccatctgacg ggtcgcggcg agcttctgtg ccgccctgtg agacgggcag
    14821 aaaggaggct tctggggaga gcgttccacc acatcatgcg ctgaggtgac tgccgggacg
    14881 gcggtcgaca tcgacttcga ttcgctaaac aaccgaggag gaagacaatg tctgctcgtc
    14941 acaacaatcg aagagggttg agccggcgtg agtttctggg gctgagcgcg gggttgggcg
    15001 ccggcgctgg ctctgcctgg ctcctgggcg cttgtgctcc ggccgcgccg gcggccacgc
    15061 ccacggcggc cgcgaatctg atggccgaaa ccaagcacct gatcaacggc acctacaatc
    15121 ccaactgggc caacaatgtg ctcgtcgagc tggccgatgc ccagggttgg ttcaaggagg
    15181 agggcatcga gtccagggaa atcgtgatca tcgatcaagg gcagatcatc ccggccctga
    15241 tcggcgggag cctgatgacg gcccagcagg atacggatgc catcgccgca gccaacctgg
    15301 ccggcgagcc tgtctttttc atctccacct accgcgacaa agagccatgg attttcggcg
    15361 tggcgaaggg catcaactcc gcagaagacc tgaagggcaa gcaagtcagc ggcggccagc
    15421 tcggctcgcg caacgagaac aacggcaagg agatgctgcg ccgcctggga ctggatcctg
    15481 agaaggacgt ggagtgggtg ccggtcggcg gcggctcgga cgggcgcgtg cagggcgtga
    15541 tcggcggggt gatcgccggc acggtgatgc aagaccgcca catgaagctc atcgaggacg
    15601 cgggcggcaa gatcctgtac aacgagcgcg aatcggtgcc gcaagattgc tactgcgccc
    15661 atcgctcctg gctggacaag aacgaacgca cggccatcgg cttcatcaag gccaccttca
    15721 aggcgcggga atacgtgctg gatttgagcc gcaaagacga gatcatcgaa ttcatgaagt
    15781 cgcgcggcta tgagttcccg gaggccttcg tcctgggcta cgaggatctg ttggccatca
    15841 tgagccccac cggcaccttc acggtggata ccatggtgaa gctgatccag gatgccgtca
    15901 aggcgggcac gctcgaaaag gaagtggact ggcacacttt tgtcgatctg aagtaccaga
    15961 atcaggcgtt caaggaactg ggacgcgacg atctggtgat gaacgtgtag aacgctggct
    16021 ggtcgggcgc agcgaaaccg gtccggcggg gctgaacgcg ccggaccggc ccacccatgg
    16081 agcgagggga agcctatgct caacgactgg actgaagtgc gttacgacct cgacaagtcc
    16141 gcgaacttcg tggagatatg caactctccc tggtacggcg acgccatcta cgacaggttc
    16201 tcggacgccg agatggcgcg ccgccacacc ctggcgcggg agatgatggc ccgcgaaggc
    16261 gtggcggcgc tgatcctgac cggcagcccc aacatctaca gcatgggtgg cgccgtcacc
    16321 tgggcctcgg gattgatcga cgagcgcggc atgtgccagt atatgctgct gccgcgcgca
    16381 ggcgagccga tgctgatcta cccgcacgcc ggctgccaca tcgaggccgc ccgtcgcatg
    16441 gtctcggtgc aggacgtgcg cggctcgcag ggcgggcatt ttggcaaggt gttggccgag
    16501 cgcctgcttg cgcttggcct gggcgaaagc cgcatcggga tcgtggcctg tgaccgcaat
    16561 ggcccggaat acatgggcat tagctgctat cgcgagttga cggagcacct gccgagggca
    16621 gagttcgtgt ttctgccggg cctgctgcac gaactcactt atctcaagag caccgaagag
    16681 atcggagcca tggcccgggc cggcgagctg gcggtggagg ccatggacgc cgtggctgcc
    16741 gcagcgcggc cgggcgctcg tgaatatcaa ctggaatccg ccggcacctt cgccatcatg
    16801 aacggcggcg gccgcgtgca cctgatgatg atcggcacga cctcgatgca tgatccgcgg
    16861 ctggtctttc ccaacccacg cccgtctcac cgcatcctgc aggagggcga catcgtcgtg
    16921 ccggagatcg ttgcgatgta caaaggctac tcggccaaga tcggccatcc ggtgaccatc
    16981 ggcccgccga cgcaagaggt cgacgccttc ttcaaagagg tgacgctggg cggctttcac
    17041 gcgctgcgcg aacaactgat gccgggcaag acgctggagg acgtgcgcca ggcgggggct
    17101 tactttcggc gacacggggc gcaggcccgg ccactgatgg tgcacggtct ggacctgatc
    17161 acagctacgc cgtacatctc ggtggatcag gtcaaagccc ggccggccga aatgaccatc
    17221 gtgcctggca tgacgttcgc catcgagatc acgcccatca atcccgatgg cacgtttggc
    17281 atgttcatgg gccgcaccta cgccatcacc gaagacggcc agcgggactt gaccccttat
    17341 cccatggacg aaatcatcgt cgcgccggcg tgagtggcat aacagcggat ggcgtgaatt
    17401 cagcggatgg gaggcggatg gtgtgaattc agcggatagg agagtgacat gcgtctggag
    17461 ggtatggcat gaaagaggaa gtccgtctgg aacgtctctc acagcctgaa gccgaggcgc
    17521 ggctgaaggc gagccgcacg gcggtgcttg cgccgggcag catcgaacaa cacggcccgc
    17581 acctgccgct gggcacggac gcgtttgcgg ccgacgcgct ggctgaagaa gtggcgcgca
    17641 agctcgggag tgtgctggtg cccatgccgc tggtcggcgt ctcgccctat catctatcgt
    17701 gggcaggcag cctctcgttc aagccgcaga cgatgatggc ggtggttgag gatatttgcg
    17761 aatgcctggc ggcgcacggc gtggagcgca tcctgttggt caactggcac gaaggcaaca
    17821 cgccggcact ccggctggcc ggcgacaacg tgcagcgcaa gttcaacctg cgcgtggtgg
    17881 tggccgagtc gcacctcatc acgcatcaac tctttggcga gcgcgcccag ctcacgcatg
    17941 ccggcaacat ggagacggcg gcggtgctga tggtcgaccc gtcgctggtg gacctgagca
    18001 aggcgacgaa ccccacgccg catgatgaag gtgacgaggg gcatcgaatc ttccgccagc
    18061 gagacgttta cccaatcatg cgcgactttc acgaggtggc gcgcacgggc tggtacggca
    18121 agccggaaga ggccacgccc gagctggcgg ctgagatccg ccagaaggta tcagacacca
    18181 ttgtcgaacg ggcgcgaatc gaattcgaac gcgagcggca tatcccacac tgaaaaggag
    18241 ctgtcttgaa cagtattccc accacgatgc gggctgccgt cacccgcgac tggaatgata
    18301 ttcgcctgga acaagtcgcg attcccgagc tggaaccggg tgaggtgctc gttcgcgtcg
    18361 gggcctgcgg tatctgcggc acggatctga aaatcgtgtc gggcgtatac aaaggctcgt
    18421 ggccgccgtc gctgcccttc atccagggtc acgaatgggg cggcgcggtc gcggcgctgg
    18481 gcgagggcgt cacgagtttg caggtgggcg atcgcgtcgc gtctgagaac cacaaaggct
    18541 gcggggcgtg tgccatgtgt cgccgaggcc gctacaacct gtgcgaggtc gcccgcacga
    18601 aaggcaaggc atacaagctg tacggccact cggcgcaagg cgccttcgcc gaatacgccg
    18661 cacggcccgc cggcctgttg cacaaactac ccgactcggt ctcgttcgag gagagcacca
    18721 tcgtcaatca aggcgcgctg ggcctgcatg ccatccgccg ctgccgcatc gatcccggcg
    18781 acacggtcgc ggtcatcggc
//
LOCUS       DBNHXB010000013         8805 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4196882, whole genome shotgun sequence.
ACCESSION   DBNHXB010000013 DBNHXB010000000
VERSION     DBNHXB010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8805
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4196882"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..529)
                     /locus_tag="AC162G_00465"
     CDS             complement(149..529)
                     /locus_tag="AC162G_00465"
                     /inference="COORDINATES: protein motif:HMM:NF017353.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HIR5074318.1"
                     /translation="MLYRYQEITPFTRPVVPPPPARSVVQNNRSMRLRDQHDQDQPEQ
                     LRLAGYAGVMDGAIILHGKWHELKGQARQHWGKLTDDDIARLSGKSEELAGVMQQRYG
                     YGKAQAEREINNWLRDHDKARPTA"
     gene            complement(537..812)
                     /locus_tag="AC162G_00470"
     CDS             complement(537..812)
                     /locus_tag="AC162G_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR5074319.1"
                     /translation="MNIIVYLIAGGIIGWLASIVMKTNAQQGILMNVIVGVVGAFVAG
                     WFLTPLFGVGTINQGDFSMPALLVSLGGAILLLAVVRLFSRGGLRMR"
     gene            complement(1091..1390)
                     /locus_tag="AC162G_00475"
     CDS             complement(1091..1390)
                     /locus_tag="AC162G_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074320.1"
                     /translation="MSSYGISSVRYDARREYATELRIHMHDGALYFPSSGWWKRDNVL
                     RGLSISDQFVTIMPAPGDKYQKDEDIHVVTVSGTRYLRIDNELVPADDLGKLLEG"
     gene            complement(1668..2102)
                     /locus_tag="AC162G_00480"
     CDS             complement(1668..2102)
                     /locus_tag="AC162G_00480"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR5074321.1"
                     /translation="MSEQENIKAAHAFFDAWNAGDLSKTAPYEADNFMAEGPGAAGPM
                     NREQNRIYSQNFLTAFPGSKFEVLLTIAQGDYIVTHWKAGGGAHTGPLTTPSGGTIPP
                     TGKRATVTGSTTSQIKNGKITRSWTFWDMASLLGQLGLLPPM"
     gene            complement(2221..2658)
                     /locus_tag="AC162G_00485"
     CDS             complement(2221..2658)
                     /locus_tag="AC162G_00485"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5074322.1"
                     /translation="MAKIESSIVIKRPTEEVFAVLSNSENNPKWQSGSLENKKTSNGP
                     IGVGTTWHSVSQFLGRRIESESEITEYEPNRKYTLKSKLPFPVEARTTLERVEGGTRI
                     NLKTEAEIGGFFKLAEPLVVSMGKRQFEGDLANLKDLMEAHAV"
     gene            complement(3027..3470)
                     /locus_tag="AC162G_00490"
     CDS             complement(3027..3470)
                     /locus_tag="AC162G_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5074323.1"
                     /translation="MNPDAIDMVSHIHSRGSSPATTTAEGAILVIDDERLMREALIDI
                     LGALTEMEVYTASNGREGLQIFQQLRQSITVVLLDLDMPVMNGEETYARLRQIAPQVK
                     VIVSSSHSPAEARLRFGEHELPAFLQKPYDTSTLLNAVQTVSAIM"
     gene            complement(3511..3873)
                     /locus_tag="AC162G_00495"
     CDS             complement(3511..3873)
                     /locus_tag="AC162G_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5074324.1"
                     /translation="MPRVFLVDGQSALRSALRLLLLDLNMQVVGEAADWSTALAQAPA
                     THPDMLVVHWGLLPPEAGAALAELRVACPVVRVVVLSGQLGVRQAALSAGVDAFISIG
                     EAPDRVAERLRAAAGAPA"
     gene            complement(3963..4604)
                     /locus_tag="AC162G_00500"
     CDS             complement(3963..4604)
                     /locus_tag="AC162G_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5074325.1"
                     /translation="MTPSQPIRVMLVDDHTMVRRGLATFLKVFDDLELASEAASGEAA
                     IQLCAQVLPDVVLMDMVMPDMDGATATRAIRGQFPMVQVIALTSFKEEGLIQSALQAG
                     AIGYLLKDVSADELARAIRAAHAGRATLSPEAAQALVHAATQPPAPGHDLTERERAVL
                     ALMVEGLNNTEIAERLVVSPSTIKSHVSNILSKLGVTSRTEAVALAVRHRLIA"
     gene            complement(4601..6331)
                     /locus_tag="AC162G_00505"
     CDS             complement(4601..6331)
                     /locus_tag="AC162G_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR5074326.1"
                     /translation="MPPSPDFYALLSSLFHQTARAPDAPPVIPLERWPAGSQEQRLLE
                     DLQAALGALHARSQTLQSQAEEKERPYRSIFEAASDGLIINDVETGLVLDANPAAGAM
                     HGYAREEFIGLHPTAFIHPESRHLFIEYVQAVQSRGVFEALAVHVRRDGSPFYVEVRG
                     TAFTYQSRRCLLGVVRDVSQRVQAEQLLQQRVEARTREQSTLLEISQTLASALELKPG
                     LILDQLRVLVEYTHAMLFALEDSALVALTVRGPQRLEQAMPFRIRLDGPETLAALFNG
                     ERPNRIADVWSADPAAQFLRSLLTDQAAVLLEGVKAWMWVPLAVKGRVIGVVSVAHTT
                     PDTFTTQHADLALTIANQAAVAMVNAELHEQAQTLAALQERQRLARNLHDAVNQSLFS
                     AGLIAEVLPRLWERDPDEGRRSLEDLRRLTRGALAEMRALLAELRPSVLTDTDLGDLL
                     RQLGNALTGRTNIPVAVTVVGQRALPADVQVAFYRVCQEGLSNIAKHATANQVAIHLQ
                     VDAGAVELRIRDDGCGFDPRHVSPGRSGLSIMRERAEAVGAVLLVTSQPDHGTEIALR
                     WTVPSEQEAR"
     gene            complement(6650..6955)
                     /locus_tag="AC162G_00510"
     CDS             complement(6650..6955)
                     /locus_tag="AC162G_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR5074327.1"
                     /translation="MEAKLYVGNLSYTTSDDDLRTLFAQAGTVVSVAVIKDRHTGTSK
                     GFGFVEMTSQVEAQKAISMFNSYQMGERALTVNLAKPREERSGARRSGSGGSDRFRR"
     gene            complement(7463..>8805)
                     /locus_tag="AC162G_00515"
     CDS             complement(7463..>8805)
                     /locus_tag="AC162G_00515"
                     /inference="COORDINATES: protein motif:HMM:NF015714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR5074328.1"
                     /translation="VLWATYLGRQATALNSLFIAAILMTLLDPFTLGDVGFQLSFAAT
                     LGLVLYTRPLQNAAERGLARWTGPERAKKTVGVLSDAVIVTLAAQITTLPILVVTFRQ
                     ISLLTLIVNALVLPAQTGVMLFGGLAMLAGMVFLPLGQVVGWAAWLFLAWTIQVIHLF
                     AQVPGATIELGYVDPLWGVAYVVALAALTFYVSRNTEQRAGIRRRMTKMISLRLALPA
                     IGIVVLLGAVALSWQPDGKLHVHALNVDGGPVFVQTPTGKQILIGGSNSPSALLAALG
                     SRMPFWDRDIDVVVVTSDDAKSMNGLLAVVDRYYVGAILSVDIGDTRAGREWLDVIAA
                     KNINVIEPGSGLGVADGIALTLDKGGWVKIETGATSISIGTPNGDSHIDVIVLAEVTD
                     ETVVWIKSNQPAIVVTRSPVTPERLVERIAFVNVEIRSAELIFDGVQWEVSAAP"
BASE COUNT         1658 a         2742 c         2646 g         1759 t
ORIGIN      
        1 ccaagtgtgg cgccaacttg ctgggcttct tctgcgctaa tccgacgttt gttgctcacg
       61 atgtacctcg ctctcaataa catgtttcta cccagtgcgc ggcccggaat gaagcccgtg
      121 cgccgcccaa cctgtggtcg tcctgtgttt aggctgtggg gcgcgctttg tcgtggtcgc
      181 gcagccagtt gttgatttcc ctttccgctt gtgctttgcc gtagccatac cgctgttgca
      241 ttacaccggc gagttcttcc gacttgccac tcagtcgggc gatgtcatcg tcggtgagtt
      301 tgccccagtg ctgcctcgct tggcctttga gctcatgcca cttgccgtgc aagatgatag
      361 ctccgtccat gacaccagca tagccggcaa gccgaagttg ttctggttga tcttgatcgt
      421 gctgatcacg aaggcgcatc gaccggttgt tctgtactac gctgcgggcg ggcggcggcg
      481 gaacaaccgg tctcgtgaac ggggtgatct cttgatagcg ataaagcaca accccgttaa
      541 cgcatgcgca gccctccccg gctgaagagt ctgacgaccg ccagtaggag gatggcgccc
      601 cccaacgaca ccagcaaggc cggcatgctg aagtcgccct ggttgattgt gccgacacca
      661 aacagagggg tgaggaacca accggccacg aacgcgccca cgacgcccac aatgacattc
      721 atcagaatgc cctgctgtgc gttcgtcttc atgacgatac tggccagcca gccgatgatc
      781 cctccagcga taagatatac aatgatgttc attttgtcct ctcctgtgtg agtgttgggc
      841 atacaccggt tccccgtgta tgccagtcta tccgcgctct ggcgcctctc ctgtcctgca
      901 taagggcatc ggccagatag acacgggtca tgagtccatg ctacaacgaa tactcctcga
      961 ccacatccat cggccgggga gtatttggct ccgccagttg ggggagatta tttggtttga
     1021 agcttctttg attgccattc agcgagcagc atattgttac tccttatgcc tgacggagtg
     1081 tgggcgcaac tcacccctcc agcaacttcc cgaggtcatc ggctggcact agctcgttgt
     1141 cgatcctcaa gtaccttgtg ccactgaccg ttacgacgtg aatatcttca tccttctggt
     1201 acttatcgcc gggcgctggc atgatcgtca caaattggtc gctgatactc agcccacgta
     1261 acacgttgtc acgcttccac cagccactgc tggggaaata gagcgcgcca tcgtgcatat
     1321 gaattctcaa ttctgtagcg tactcccgac gcgcatcata gcggacgctg gagatgccgt
     1381 agctggacat gttctcccct ttctgggatc gtgattcaaa ggcttagcat gacggcgaaa
     1441 gtcctgagaa aataagtccg cttccacgcc ctctacacga ggttgcggct ctgtgggggc
     1501 cattgtcttc gggcagccgc catgaatatg ggacaagccc cgcctatcct tcagctaagc
     1561 tgccccagct ctatgccggc gctccgggca gcttcctgtt ggggcaggcg gttgcgtgaa
     1621 gcgcgctgcc ccaacagcgt gtcttacagt gaagcgacgg actcttctta catgggcggc
     1681 aacaagccca actggcccaa gagtgaggcc atgtcccaga atgtccaaga gcgagtgatc
     1741 ttgccgttct tgatctgtga cgtagtgcta cctgttactg ttgccctctt acccgtaggc
     1801 ggaatggtgc cgcccgaggg ggtggtcagc ggaccggtat gtgcgccgcc gccggctttc
     1861 cagtgcgtga cgatgtagtc gccttgcgcg atggtgagca gcacctcaaa cttagagccg
     1921 gggaaagccg tcaagaagtt ctggctgtat atccgatttt gctctcggtt catcgggccg
     1981 gctgcgccag gcccctccgc catgaagtta tcggcttcat agggggccgt cttgctcaag
     2041 tcgccggcgt tccaggcgtc gaaaaaggca tgtgcggcct tgatgttctc ttgctcagac
     2101 atggtttgtt ctctcctgat gtgataggtg tgcgcctcac ggcgaggcgc acactagcga
     2161 ttgactcccg gcgatagttg ggtgcagctt tacgaatgcg cccagggtag cccacacact
     2221 ctagacagcg tgagcttcca tcaggtcctt caggttggcg agatcgcctt caaactgtcg
     2281 cttgcccatg ctcacgacaa gcggctctgc cagcttgaag aagccaccga tctccgcttc
     2341 agtcttgagg ttgatccggg tgccaccttc aacacgctcc aaggtcgtcc gggcttcaac
     2401 cgggaaaggc aatttgcttt tcagcgtgta ttttcggttc ggctcgtact cggtgatctc
     2461 ggattcgctt tcgatccgcc ggccgagaaa ctggctgact gagtgccacg tggtgcccac
     2521 cccaatcggg ccgttcgaag ttttcttatt ctcgagactt cccgactgcc acttcgggtt
     2581 attctcactg ttgctcagaa ctgcaaagac ctcctcggta ggtcgcttga tcacgatact
     2641 ggattcgatt tttgccattt cgttattctc cgtcagatag agcatagggc gtgaatgaca
     2701 gcgacatggt agcttcactg agtccataga cgccgaccgc agctcaacct cgaactgcgt
     2761 tggattgggc tgcatctgaa tcgcactgca acctatgcgc cctgtctcgt agaggctacg
     2821 ccggtcggtg accgtgatgt aggcagcctg tcgtgccatg ccattgccgt cattgcccag
     2881 caacggggtg acggttgtgg cgcttcagac gccattgtag tgaagagcgg cccaacgcag
     2941 gtatcaggcg cgaagcgaag cgcgccgggt ttcaatccgc tcctccgcct tgaggctaac
     3001 ctcggctagc agaaagcaac cgggtgctac attattgccg acaccgtctg tacggcattg
     3061 agtagggtgc ttgtgtcgta gggcttctgc aagaaagcgg gtaactcgtg ttccccaaaa
     3121 cgaagccgtg cctctgcagg gctgtgactc gatgagacaa tcaccttaac ctgaggcgcg
     3181 atttgccgca gccgggcgta agtctcttcg ccgttcatca ccggcatgtc caggtcgagc
     3241 aagaccacgg tgatgctctg gcgcagctgc tgaaaaatct gcaagccctc gcgtccattg
     3301 gatgcagtgt acacttccat ttctgttaga gcgccaagga tatcgattag cgcttcgcgc
     3361 atgagacgct cgtcgtcaat aacgagaatg gcgccttcgg ccgtggttgt ggccggggag
     3421 ctgcctcggc tgtgaatatg gctgaccatg tcgattgcat ccgggttcat gctgtgatcc
     3481 tcctggacct tcggaatcag ttgcccccgt ttacgccggc gctcccgccg cggctcgcag
     3541 gcgttcagcc acgcggtctg gcgcctcgcc tatgctgata aaggcatcga ccccggcgga
     3601 gagcgcggcc tgacgaacac ccagctggcc gctgaggacg acgacgcgca caactgggca
     3661 agccacgcgc agttcggcca gggccgcgcc ggcctctggg ggaagcaagc cccaatgcac
     3721 caccagcatg tctgggtggg ttgccggggc ctgggctaag gcagtagacc agtcggcggc
     3781 ttcaccgacc acctgcatgt tgagatccag cagcagcagc cgaagggccg agcgcagtgc
     3841 ggattgacca tcgactaaaa atacgcgggg catgagttca tgctacaacg aatacgcccc
     3901 ggccacatcc atcggccggg gagtatttga ctccgccagt tgggggagat acagaggggg
     3961 tgtcaggcga tgagccggtg gcggacggcc agcgccacgg cttcggtgcg gctggtgacg
     4021 cccaatttgg agaggatgtt gctgacgtgg gatttgatgg tggacgggct gacgaccagc
     4081 ctctcggcga tctcggtgtt gttcaagcct tcgaccatca gagccaaaac ggcacgctcg
     4141 cgctccgtca ggtcgtggcc cggcgccggc ggctgggtgg cggcgtggac caatgcctgg
     4201 gcagcctcgg gagacagggt ggcgcggccg gcgtgcgccg cgcggatggc ccgggccagt
     4261 tcgtcggccg acacatcctt gagcaaatag ccgatggccc cggcctgcag cgcgctctgg
     4321 atcagcccct cctctttgaa actcgtcagc gcaatcactt gcaccattgg aaactgcccg
     4381 cggatggcgc gcgtggcggt cgccccatcc atatccggca tgaccatatc catcagcacg
     4441 acgtcaggca gaacctgggc gcagagttgg atcgcggcct cgccgctggc cgcctcgctc
     4501 gccagttcca ggtcgtcaaa gaccttcaag aaggtcgcca gtcctcggcg caccatcgta
     4561 tgatcgtcga cgagcatcac gcgaataggc tgggaggggg tcatcgggcc tcctgttctg
     4621 agggtactgt ccagcggagg gcgatttcgg tgccgtgatc cggctggctc gtgaccagca
     4681 acaccgcgcc cacggcttcg gcgcgctcac gcatgatgct caggccagag cgaccgggcg
     4741 atacgtgcct aggatcgaaa ccgcagccgt cgtcgcggat gcgcagttcc accgcaccgg
     4801 cgtccacctg cagatggatc gccacttggt tggccgtggc atgcttggcg atgttgctca
     4861 gtccttcctg gcacacacgg tagaacgcca cctggacatc ggcgggcagg gccctttgcc
     4921 ccaccacggt cacggcgacg gggatgttcg tccggcccgt gagcgcgttg cccaactggc
     4981 gcagcaggtc gcccaggtcg gtgtcggtca ggaccgaggg ccgcagctcg gccagcagcg
     5041 cgcgcatctc ggccagtgcg ccgcgcgtca accggcgcag gtcctccagc gaccgccggc
     5101 cctcgtccgg gtctcgctcc cacaagcgcg gcaggacttc ggcgatcaga ccggcggaga
     5161 agagcgactg gttgacggca tcgtgcaggt tccgcgccag acgctgacgc tcctgcagcg
     5221 cagcgagcgt ttgtgcctgt tcgtgcagtt ctgcattgac catggcgacc gcggcctggt
     5281 tggctatcgt taaggccagg tcggcgtgct gggtcgtgaa ggtgtccggc gtggtgtggg
     5341 cgacgctcac aaccccgata acgcggcctt tcaccgccag ggggacccac atccacgctt
     5401 tcaccccctc caacagcacg gctgcctgat cggtcaggag cgaccggaga aactgcgctg
     5461 ccgggtcggc gctccacacg tcggcgattc ggttgggtcg ctccccgttg aacaacgccg
     5521 ccagggtctc cggaccgtcc agccggatgc ggaagggcat ggcttgttcc agtcgctgcg
     5581 gcccgcgcac ggtcagggcg accagggctg agtcttccag cgcgaagagc atggcatgcg
     5641 tgtactcgac aagcacacgc aattggtcga ggatcaaacc cggcttgagt tccagggcgg
     5701 aagccaatgt ttgagagatt tccaataggg tggactgctc ccgcgtgcgg gcctccactc
     5761 gctgctggag gagttgctct gcctgaaccc gctggctgac gtcgcggacg acacccagga
     5821 gacaccggcg gctctgataa gtgaacgccg tcccacgcac ctcgacgtag aacggtgagc
     5881 cgtcccggcg aacatggacg gcaagcgctt caaacacgcc gcgggactga accgcctgga
     5941 cgtactcgat gaacaggtga cggctttcgg ggtggatgaa tgccgtcggg tgcaggccga
     6001 tgaattcctc gcgggcatag ccgtgcatcg cgcctgccgc gggattggcg tcgagcacca
     6061 ggccggtctc cacatcgttg atgatcaacc cgtcgctggc ggcttcgaag atgctccgat
     6121 acggccgttc tttttcctcg gcttgagact gaagggtctg gctgcgggcg tgcagcgcgc
     6181 ccagcgcggc ctgaaggtct tccagcaggc gctgttcctg agatccggca ggccagcgct
     6241 cgagagggat gacgggaggc gcgtctggag cgcgagccgt ttggtggaac aggctggata
     6301 acagcgcata gaagtcgggc gaaggaggca tagagtatag tataactcag agcggcgcgg
     6361 ctgtcactcg ccggcaccgt cgccgtatct gcatgcggga tatgcgccag cagatgatgc
     6421 acacctgcgc tgcgccgcac gcagttctgt agttgacgca cacttcgcga agtgcgtcgg
     6481 cggccggcat gcttgcacct gcccacgggt tttaagaggg tctcttggtt accgcctggc
     6541 caattaacgc acggaggcgg tgacacagat caccgcctcc gccatatcct aaaggccgat
     6601 ttcggccttg acgctgaaag cgccatggca cgctacgagg gcagactgct taccgccgga
     6661 accggtccga tccaccgctg ccggaacgtc gcgcgccgct tcgctcttcg cgcggcttcg
     6721 ccaggttcac cgtcagcgca cgctcaccca tctggtagga gttgaacatg ctgatcgctt
     6781 tttgggcctc gacctggctg gtcatctcga cgaacccaaa gcctttcgag gtgccggtgt
     6841 ggcgatcttt gatcaccgcc acggatacga ccgtgcccgc ctgcgcgaac aacgtgcgca
     6901 gatcgtcatc ggaggtcgtg tacgacaagt tgcccacata cagttttgct tccatttccg
     6961 agtctcctta gtgaccggct gtgcgccgga aagaaaacgc cgccagaccg taagagagtc
     7021 tggcggcaag cgacacactc aaatccagtt gtacctggtt atattatacc acagatcgtg
     7081 gttttgtgtc cgcacgcaga ctatcagcta tcagccgcaa ggcaatcaca tctcaaacgc
     7141 cgagggctgt gcgaagataa tccgcgacct gtggtacgtt ccttgtcaga gaggcagcaa
     7201 ggtttagccg ccctgctctg ccgcaggcgg gccagtggaa ccgcgcgtga gggttctgca
     7261 gaatcccgca ggatacgaac ctccgtcccg caggttatga gctccactca caaagcgtat
     7321 atcacagggc attcgagaca aagcaaacac cgcagatttt cactctgcgg tgtcgtctga
     7381 gtagcggggg gtggattaga acctgcggcc tccgggtgat gagcccgacg caattgccac
     7441 ccgtggccca acgtcggcag cctcaaggcg ctgcactcac ctcccactgc accccatcga
     7501 agatcaactc ggcggagcga atctccacgt tgacaaacgc gatgcgttcg accaacctct
     7561 ccggcgtaac cggcgatcgc gtgaccacaa tggccggctg attcgatttg atccacacga
     7621 cggtttcgtc tgtcacctca gccagtacta tcacgtctat gtgcgaatcg ccgttcggtg
     7681 tgccgatgct gatggatgtc gcgcccgtct cgatcttcac ccagccgcct ttgtccagcg
     7741 tcaacgcgat gccgtccgct acaccgagcc ccgatcccgg ctcgatcaca ttgatgttct
     7801 tcgccgcgat gacatccagc cactcgcgcc cagcgcgcgt atctccgatg tcaaccgaca
     7861 gaatcgctcc gacgtaatac cgatccacca ccgccaacaa tccattcatc gatttcgcgt
     7921 cgtcgcttgt cacaaccacg acgtcgatgt cccgatccca gaacggcatc cgcgacccga
     7981 gcgccgccag cagcgccgac gggctgttcg agccgccgat caggatttgt ttgccggtcg
     8041 gcgtctggac gaaaaccggt ccgccgtcga cgttcaacgc atggacgtgc agcttgccgt
     8101 ccggctgcca cgacagcgcg accgcaccca gcagcacgac gatgccgatg gcgggtaagg
     8161 ccaggcgaag agagatcatt ttggtcatac ggcgccttat ccccgctcgc tgttccgtat
     8221 tgcgtgaaac gtaaaacgta agggcggcta gagcgacaac atacgccaca ccccacagcg
     8281 gatcgacgta gcccaactcg atcgtcgcgc cgggcacttg cgcgaacagg tgaatcacct
     8341 ggatcgtcca ggccaggaac agccacgccg cccagccgac gacctgtccg agcggcagga
     8401 agaccatgcc cgccagcatc gccagcccgc cgaagagcat cacgcccgtc tgcgccggca
     8461 gcaccagtgc gttgacgatc agcgtgagga gcgagatttg ccgaaaggtg acgaccagga
     8521 tcggcagcgt cgtgatctgc gcggcgagcg tcacgatgac ggcgtcgctc aacacgccga
     8581 cggtcttctt cgcgcgttcc ggtccggtcc agcgcgccag tccgcgctcg gcggcatttt
     8641 gcagcggacg cgtgtacagc accagcccca acgtcgccgc gaagctgagc tggaagccga
     8701 cgtcccccag cgtgaacgga tcgagaagcg tcatcaggat cgcggcgatg aagagcgaat
     8761 tgagcgcggt cgcctggcgg ccgaggtaag ttgcccatag cacca
//
LOCUS       DBNHXB010000014         2573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1009816, whole genome shotgun sequence.
ACCESSION   DBNHXB010000014 DBNHXB010000000
VERSION     DBNHXB010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1009816"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="AC162G_00520"
     CDS             complement(<1..331)
                     /locus_tag="AC162G_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5074329.1"
                     /translation="MQTVIKPRASKRVLIIDDDIDAAHTLFMLLLEMGHAPAYATSGR
                     AAIAQATKLRPEFVFLDIGLPDRDGSEVAKQLRQTGGCEHALIIAVTGLGNEHRERML
                     QAGCDAYY"
     gene            582..896
                     /locus_tag="AC162G_00525"
     CDS             582..896
                     /locus_tag="AC162G_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5074330.1"
                     /translation="MTRIVYVNERDVAAREIYPGVRKRVLWQGPNGAKAQILEIDAGA
                     KFLKLDVHHPGPEEIYVVSGTFNDGARDYPAGSFVHNPAGSSHLPQSRQGCVLFVFFP
                     EG"
     gene            complement(778..1815)
                     /locus_tag="AC162G_00530"
     CDS             complement(778..1815)
                     /locus_tag="AC162G_00530"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR5074331.1"
                     /translation="MDSPETLADIAIFAAVIREGSFTRAADKLELSTSQVSKCVNRLE
                     RNLGARLLHRTTRRLRLTEAGSALYENSSTALAAIEDAKLAVSKLQGAPRGKLKVSTS
                     TAFGTMQLPRVIRDLSAQYPELEVELVLEDRHVDLVREGIDVAIRITGEAPDSGLVYR
                     KLAPNRQVICASPDYIGRRGLPRTPDDLANHNCIAHSARTTPRIWHFTGPDGGQISVP
                     INGRPTINSALAVRQAALEGLGVIELNSYLVGEDIAAGRLARLLPQYQPKELNFYAVY
                     AERRFLAPKIRVFVDAMLARMSPEPPWDSFLNLPEKTRIERTPASTAEDATIRQGYGR
                     KNQPGNRARRR"
     gene            1915..2082
                     /locus_tag="AC162G_00535"
     CDS             1915..2082
                     /locus_tag="AC162G_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074332.1"
                     /translation="MATIQVVLGLLCLTHPLLPGVGVSLAACAIGLGLIVSGLLAGVD
                     DEEAHDLDFSR"
     gene            2093..>2573
                     /locus_tag="AC162G_00540"
     CDS             2093..>2573
                     /locus_tag="AC162G_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HIR5074333.1"
                     /translation="MEHVHHAHHQHHARQPSLNRTAWSATLHCLTGCAIGEVLGMVIG
                     TALGWGNGATIALAVALAFLFGFALTLLPLLRATGFNTALKLALASDTASITVMEIVD
                     NAIMLVIPGAMDAGLGTLLFWGSLAFALGVAAIAAFPINRWLIARGKGHAVVHAYHHH
                     "
BASE COUNT          448 a          788 c          797 g          540 t
ORIGIN      
        1 cgtagtacgc atcgcagccg gcttgaagca ttcgttcgcg atgttcgttt ccgaggcctg
       61 tgacggcgat tatcagcgcg tgttcacatc ctccggtttg tcttagctgt tttgctacct
      121 cgcttccatc ccggtccggg agtccgatat ccagaaagac gaattccggc cgtaacttcg
      181 tcgcctgtgc aatcgccgcg cgtccgctcg tggcgtaagc cggagcgtgc cccatttcca
      241 gcaggagcat gaaaagggtg tgcgccgcat cgatgtcatc gtcgatgatg aggactcgtt
      301 tgctcgcgcg cggtttgatc acagtttgca ttttgtatca acggaggtat cacgctcaaa
      361 actgctatta gcgcatcttc gctccgatca gtcgctggcg taccgcacag ctctttaccc
      421 tgattccgta gtgcgttggc tacatctgta gccattgcgc cgcttcaacg cggtgagcct
      481 cgccgcccac taggcgttcg cgaggattgt gcatatgcgc gcaaagtgat tttcggggtg
      541 caaatcgatt gtcgccgagg atatcgggac aatgcgcgcg gatgaccagg atcgtttacg
      601 ttaatgagag ggacgtggct gcacgcgaga tctatcccgg cgtgcgcaaa cgcgtgctat
      661 ggcaagggcc gaacggcgct aaggctcaga tccttgagat cgatgccggc gcaaagttcc
      721 tgaagctcga cgttcatcac ccgggtccgg aggagattta cgtcgtttcc ggcacgttca
      781 acgacggcgc gcgcgattac ccggctggtt ctttcgtcca taaccctgcc ggatcgtcgc
      841 atcttccgca gtcgaggcag gggtgcgttc tattcgtgtt ttttccggaa ggttgaggaa
      901 agaatcccac ggcggctcgg gcgacatacg ggccagcatg gcatcgacaa acacccggat
      961 cttgggagca aggaaacgac gttcggcgta gacggcgtag aagttcaact ctttcggttg
     1021 gtactgaggc agcagccgtg cgagccgccc tgccgcgatg tcttcgccga ccagatatga
     1081 attcagctcg atgacgccga gcccttcaag cgcggcctgg cggacggcaa gcgcgctatt
     1141 gatggtgggt cggccgttga tcggcactga aatctgcccg ccgtccggac ccgtgaaatg
     1201 ccatatacgc ggggtggtgc gggcgctgtg ggcgatgcag ttgtggttcg ccaagtcgtc
     1261 gggcgtacgc ggaagtccac ggcgtccgat gtagtccggc gaggcgcaaa tcacttgccg
     1321 gttgggcgcg agcttgcgat agaccagccc cgagtcgggc gcctcgcccg taatccggat
     1381 cgcgacatcg attccctccc tgacgagatc aacgtggcgg tcctccagta ccagctcgac
     1441 ctcgagctcc gggtactgcg cgctcaggtc gcgaatgacc cgggggagct gcatcgtgcc
     1501 gaacgcagtt gaggtgctga ccttcagctt gccacgtggt gcaccctgaa gtttggatac
     1561 ggcgagcttc gcgtcttcga tggcggcgag cgctgtcgag ctattctcgt agagcgccga
     1621 gccggcctcg gtaagacgaa gccggcgcgt cgtgcggtgc agcagccggg cgccgaggtt
     1681 acgttccagc cggttgacgc acttgctgac ctgcgaggtt gagagctcca gtttgtctgc
     1741 ggcgcgcgta aagctgccct cgcgtattac cgcggcgaag atcgcgatgt cggccagcgt
     1801 ctccgggctg tccattgttt ccgattgtgc ataaagaatt tccccatcgc cagatgatct
     1861 ccgcggcgcg cgggcgaaca attcacaccg gttcgatcga aaggaggggc acacatggcg
     1921 acgatccagg tggtactagg actgctctgc ctcacccacc cgctgcttcc cggcgtcggt
     1981 gtcagcctcg ccgcatgcgc catcggcctg ggcctcatcg tcagcgggct tctcgccggc
     2041 gtcgacgacg aggaagccca cgacctggac ttctcgagat aaggaaatcg acatggaaca
     2101 tgttcatcac gcgcatcacc agcaccatgc gcgccagccg tcgctgaatc gcaccgcgtg
     2161 gtcagcgacg cttcactgcc tgactggctg tgccatcggc gaagtgctcg gcatggtcat
     2221 cggtaccgcg ctcggctggg gcaacggcgc cacgatcgca ctcgccgtgg cgctcgcctt
     2281 cctgttcgga tttgcgctga cgcttctgcc gttactcagg gcgaccggat tcaacacggc
     2341 cctgaagctc gccctcgcgt ccgataccgc ttccatcacc gtgatggaga tcgtggataa
     2401 cgcgatcatg ctggtcatcc cgggggcgat ggatgccggt ctgggcacac tgctcttttg
     2461 gggcagcctc gctttcgcac tcggcgtcgc cgctatcgcg gcgttcccga tcaatcgctg
     2521 gctgatcgcc cgcggcaagg gccacgcggt ggtccatgcc tatcaccacc acc
//
LOCUS       DBNHXB010000015         4578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3694394, whole genome shotgun sequence.
ACCESSION   DBNHXB010000015 DBNHXB010000000
VERSION     DBNHXB010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3694394"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..372
                     /locus_tag="AC162G_00545"
     CDS             94..372
                     /locus_tag="AC162G_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014643.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR5074334.1"
                     /translation="MTQRYSIAEARHNLDALVHELERKAHIELTRGGEPVAILLSMRE
                     YQRLTAEKRGFWQAYESFRRAVDLPRLKIEPDVLEGVRDRSAGREMRW"
     gene            369..788
                     /locus_tag="AC162G_00550"
     CDS             369..788
                     /locus_tag="AC162G_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006526959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR5074335.1"
                     /translation="MSARYLLDTNVLSEPLRPAPHSKVIERLRRHQDELATASIVWHE
                     LWFGCRRLPDSAKRQAIEDYLTHVIAPSLPILPYDERAAEWHAAERARLTQTGKTPAF
                     ADGQIAAIARTQDLILVTFNRADYAEFQDLTLEDWRR"
     gene            complement(813..1601)
                     /locus_tag="AC162G_00555"
     CDS             complement(813..1601)
                     /locus_tag="AC162G_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012945370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIR5074336.1"
                     /translation="MSHDQRFTVQPFPSARGIVVDAGRLAMRRHIVHGLLELDVTRAR
                     EFMRAHKARTGEPLSFTAFVVACFARAIDRHKPVHAYRDWRNRLVIFDDVDVVTMIEA
                     ERGGVAMAHIARAANARSVFDIHREIRSIQARPAKSDQHKWLAGIGPRVPAFVRDVFY
                     WALRKNPHWFKRYAGTCVVTSVGMFGSGGGWGVVFLPMHTVGLTIGGIAQKPGVVDGQ
                     IVIREYLDLTLSFDHDIVDGAPAARFAERLRQLIEGADGLDPGP"
     gene            1923..2441
                     /locus_tag="AC162G_00560"
     CDS             1923..2441
                     /locus_tag="AC162G_00560"
                     /inference="COORDINATES: protein motif:HMM:NF016001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HIR5074337.1"
                     /translation="MAHSRRIPIWLWRWMHRLNRRISGGYRRGARLGRLVLLLTTTGR
                     KTGLPRTTPVQYEIVDGAIYVASARGQEADWFRNIVANPRVRVQIKDEQFEALAEPIT
                     DPTRVADFLELRLKRHPVMIRLMLLAEGLPLRFNRTALETFAADKAVVAIRMPQVPAT
                     PGKTSVDAQEAT"
     gene            2632..3507
                     /locus_tag="AC162G_00565"
     CDS             2632..3507
                     /locus_tag="AC162G_00565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR5074338.1"
                     /translation="MRRSQSSLTAAGIAIVRAIESEKPESERICYDPYARRLVNSALF
                     HLVRFFDRLGYGEWRGPGVMGFLAARDRHIDEYLMVCLAEGRDQLVILGAGYDARAYR
                     FEQQLKGRVKVFEVDHPATQQAKLVKLRQVFGRAPEHVVYVSIDFNTQTLAGRLSECG
                     YDERLKTLFIWQGVTQYLTPEAVDNTLAFVAHHSGPGSSIIFDYMYTSLLDGTVRHGE
                     VSKMRQDRWISGETLSFGIPEGTIESFLEQRGFGRIHNADHVFLHNAYFTGPSQKRKV
                     ADGYAIVSAVVSPAP"
     gene            3613..4326
                     /locus_tag="AC162G_00570"
     CDS             3613..4326
                     /locus_tag="AC162G_00570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR5074339.1"
                     /translation="MRTNITQPQLDTKPGITRRAIQVFATIAFLAAMLFLPSGRLDWV
                     WAWVLIGLYLLGILANFVLLMRFSPATIAERAEARGMKAWDKIVGGLWSLMYFVATPV
                     VAGLDARFGWTGSYPPALHSLGVVAFALGLALFSWAMVSNAFFATVVRIQTDRGHTVC
                     TTGPYRFVRHPGYVGGIVQSLAVPLLLGSPWALVPGGLAALLMIIRTALEDRTLREEL
                     PGYVDYAQRTRYRLVPGVW"
     gene            4465..>4578
                     /locus_tag="AC162G_00575"
     CDS             4465..>4578
                     /locus_tag="AC162G_00575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014601.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR5074340.1"
                     /translation="MRILVCGAGPLGSLFAARLQQGGHGVSILARGQRLVDL"
BASE COUNT          843 a         1463 c         1424 g          848 t
ORIGIN      
        1 cagggatcat caccgtgatc ggcggagtga tactcgttca gtggaaacca acatgataga
       61 atagccgttg aaatggctat cctggaggtc ggcatgacac aacgctactc gatcgccgaa
      121 gcccgacaca acctggacgc cctggtgcac gagctggagc gcaaagcgca catcgaattg
      181 acccgcggtg gcgagccggt ggcaatcttg ctatctatgc gcgagtacca gcgcctgacg
      241 gctgaaaaac gcggattctg gcaggcgtat gaatccttcc gtcgcgccgt cgacctgccc
      301 aggctcaaga tcgagccaga tgtgctcgag ggggtgcgcg accggtcggc cgggcgggag
      361 atgcgctggt gagcgcgcga taccttctgg acaccaatgt cctctccgag ccgctgcgcc
      421 cggctccgca ttcaaaggtt atcgaacggc tgcgccggca tcaggacgag ctggcgacgg
      481 cctccatcgt ctggcacgaa ttgtggttcg gctgccggcg cctccctgat tccgccaagc
      541 gccaggctat cgaagattat ctcacccacg tcatcgcgcc gtccctcccg atcctgccgt
      601 acgacgagcg cgccgccgag tggcatgccg ccgagcgcgc ccgcctgacg cagactggca
      661 agacgcctgc tttcgcggac ggtcagatcg ccgccatcgc caggacgcaa gatctcatcc
      721 tcgtcacgtt caaccgcgcc gactacgccg agtttcagga cctgaccctc gaagactggc
      781 gtcgatagcg ctacagcgac ttccggatgg atttacggcc cggggtcgag gccgtcggcg
      841 ccttcaatca actgcctgag gcgctcggcg aagcgcgccg ccggcgcgcc gtccacgatg
      901 tcgtgatcga aactgagcgt caggtcgagg tattcccgga tcacgatctg cccgtccacc
      961 acacccggtt tctgcgcgat gccgccgatc gtcaggccga ccgtgtgcat cggtaggaag
     1021 acgacgcccc acccgccgcc gctgccgaac ataccgaccg acgtcacaac gcacgtccct
     1081 gcgtagcgct tgaaccagtg cggattcttc cgcagcgccc agtaaaagac gtcgcgcacg
     1141 aaggccggca cgcgcgggcc aataccggcc agccatttgt gctgatcgct cttggccggt
     1201 cgcgcctgaa tcgatcggat ctcgcggtgg atgtcgaaga cggatcgggc gttcgcggca
     1261 cgggcgatgt gcgccatcgc gacgcctcct cgttcggctt cgatcatcgt gacgacatcc
     1321 acgtcgtcga agataaccag ccgattgcgc cagtcgcgat aggcgtgcac gggcttgtgg
     1381 cggtcgatgg cgcgggcgaa gcaagcgacg acgaacgccg tgaacgacaa tggctcgccg
     1441 gtgcgtgcct tgtgcgcgcg catgaactcg cgcgctcgcg tcacgtcgag ttcgagcagg
     1501 ccgtgaacga tgtgccgccg catcgccaat cgtccggcgt cgacgacgat cccccgcgct
     1561 gaaggaaaag gttgaaccgt gaatcgctga tcgtgtgaca tcgattacct ccctccccgc
     1621 ccgacccggt gagcgcaagc accgcgccgg cgatttcggt ttgatgcctt ttcaggccgt
     1681 gccttccgtc ctgaacacag ccagtataac ccggcgcttg gccgtgtctg ctgtcaatcg
     1741 tcccgctacc agactgaggc atatccatga tgtagacggc cacttgattt tagagacagc
     1801 gataagcgaa atgccatccc ggccagcgat cgtgagagtt gtcacctcga attcagacct
     1861 ccggcagatg acaagtgcgc cacaatgccg gacactgagc acagtagtcg aggagttcga
     1921 tcatggctca ctcacgccgc attccaatct ggctctggcg ctggatgcat cggctcaatc
     1981 gccgaatctc gggcggctat cgtagaggcg ctcgattggg tcgcctggtg ctgctgttga
     2041 ccaccacggg gcgcaagacc ggcctgcctc gcacgacacc cgtgcagtac gaaatcgtcg
     2101 acggcgcgat ctacgtcgcg tcggcgcgcg gacaggaggc cgactggttc cgcaacatcg
     2161 tcgcgaaccc acgcgtgagg gtgcagatca aagacgagca gttcgaggcg ttggccgaac
     2221 cgattaccga tccaacccgt gtcgccgact ttctggaact gcgtctgaag cggcatcccg
     2281 tcatgatccg gctcatgctg ctggccgagg gcctgccgct gagattcaac cgtaccgctc
     2341 tcgaaacatt cgcagccgat aaagccgtcg tcgctattcg gatgccgcag gttccggcga
     2401 cgcctggcaa gacatctgtt gacgcccagg aggccacatg actgcctgca tccccgaatc
     2461 ccaccgtgat ctgctgatcc gccccatcca cgggagcgcc cgtgagagcg ccggcttcgc
     2521 atgcggctgg atggtcggca caagaaggcg cgttaggaac ggagcgcact gtgctgcgca
     2581 gcacctgcgc cggagtcaag cgcgtcgcac gagaacaacc tggaggtcgg tatgcgcagg
     2641 agtcaatcga gtctaaccgc agccggtatc gccattgtgc gcgccattga atccgagaag
     2701 ccggagagcg aacgtatctg ctacgaccca tacgcgcgtc gactcgtgaa cagcgcgctg
     2761 ttccacctgg tgcgcttctt cgaccggttg ggctacggag agtggcgcgg ccccggcgtg
     2821 atgggctttt tggccgcgcg cgaccggcac atcgacgagt accttatggt ctgcctggcc
     2881 gagggccgcg atcagttagt catcctgggg gccggctatg atgcccgcgc ttaccgtttc
     2941 gagcagcagc ttaaagggcg ggtcaaggtg ttcgaggtag accatcccgc tacccagcag
     3001 gccaagctgg tcaagctcag gcaagtcttc gggcgcgcgc cggagcacgt cgtctacgtc
     3061 tcgattgact tcaacacgca gacgctggcc gggcgcctgt cggagtgcgg ctacgatgaa
     3121 cggctcaaga ctctatttat ctggcagggc gtcactcaat acctgacgcc cgaggcggtg
     3181 gataacacac tggcgtttgt cgcccaccat tccgggccgg gcagttcgat catcttcgac
     3241 tacatgtaca cctctctgct ggatggcacc gtcagacatg gtgaggtcag caaaatgcgc
     3301 caggatcgct ggatcagcgg tgagaccctg tccttcggca ttcccgaagg cacaatcgag
     3361 tcctttctgg agcagcgcgg ctttggccga atccacaacg ccgatcatgt cttcctgcac
     3421 aacgcctatt ttactggccc gagccagaag cgcaaggtgg cggacgggta tgcgatcgtc
     3481 tcagccgtcg tcagtccggc accctgaaaa tcctggcgct tgaacgagaa cttcccggct
     3541 gtagaagcat gaggcagacg catgaacctg cccaaactca agcagcatgt cctgcttact
     3601 ttaaggagga ccatgcgcac aaatatcacg caacctcaac tcgatacgaa gcccggcatc
     3661 accaggcggg ccattcaggt attcgccacg atcgccttcc tggctgcgat gttattcctc
     3721 ccgtcaggcc gattggactg ggtgtgggcc tgggtgttga tcggcttgta cctgctcggc
     3781 atactggcca acttcgtcct cttgatgcgc ttcagcccgg cgaccatcgc cgagcgcgcc
     3841 gaggcccggg ggatgaaagc ctgggacaaa atcgtcggcg gcttgtggag cctgatgtac
     3901 ttcgttgcca cgccggtcgt tgccggtctg gacgcgcgat tcggatggac gggatcgtac
     3961 ccgccggcac tccacagcct tggcgtggtg gctttcgcgc tcggactcgc gctgttcagt
     4021 tgggcgatgg tctccaatgc cttcttcgcg acggtcgtcc gcatccagac cgatcgcggg
     4081 cacaccgtct gcaccacggg gccatatcgc ttcgtgcgcc atccgggata tgtcggcggg
     4141 atcgtccagt cgctcgcggt accgctgctg ctcgggtcgc cgtgggcgct cgtcccgggt
     4201 gggctggcgg cgctcctgat gatcatccgc acagcgttgg aagatcggac actgcgtgag
     4261 gaactgcctg gctacgtcga ctatgcacag cggacgcgct accgtctagt cccgggagtg
     4321 tggtgatcgg ccgctggtgc catttggagg tctcaggagt gagcgccggc actttcgtta
     4381 cggacaagcc tgcatggcgt agcggcgccg caccgggtta ctgatgccga ggcggcgaca
     4441 gaagagagac acggaggatc acccgtgaga atcctggtat gcggcgctgg cccgctgggc
     4501 agcctgttcg cggcgcggct ccagcagggc gggcacggcg tgtcgatcct ggcgcgcggt
     4561 cagcgcctcg tcgacctg
//
LOCUS       DBNHXB010000016         6329 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4533038, whole genome shotgun sequence.
ACCESSION   DBNHXB010000016 DBNHXB010000000
VERSION     DBNHXB010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6329)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6329)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6329
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4533038"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="AC162G_00580"
     CDS             <1..525
                     /locus_tag="AC162G_00580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5074341.1"
                     /translation="RIEKARTLIRPGIPGPGGVWADLGCGDGIFTAALHTLTRPAAEI
                     FGVDRDLRALDALARHFAEGFPEARIRPMRADFTQPLTLPPLDGILLANALHFARDKQ
                     AALKHAAALLKPGGRLIVVEYNTARGNPAVPYPLDEMGFLELTRAIGLREGRILARIP
                     STFLGEMYAGIGVA"
     gene            complement(565..1221)
                     /locus_tag="AC162G_00585"
     CDS             complement(565..1221)
                     /locus_tag="AC162G_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5074342.1"
                     /translation="MRFVFRIPSAVNRRAFLRLAGAGVSGVLLWRLGESTVARAALPG
                     SRRRFTGSYETGSFTGVFPVTAWLLDNPLPVEVQHWRLIVDGFVERTLALTYAQLGPL
                     AHEEATATLDCTGGWYSTQVWQGITIAQLLNLAGVKPGARSLTVEGITGYGRRFSIEQ
                     ARGYVLATHVAGRPLDHGHGFPLRLVAVDHRGFDWVKWVSRIRVNDTSEVWQPPVPLQ
                     "
     gene            complement(1671..2189)
                     /locus_tag="AC162G_00590"
     CDS             complement(1671..2189)
                     /locus_tag="AC162G_00590"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF012436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HIR5074343.1"
                     /translation="MLISENLAKAFNQQVGNEFGASLQYVMLAAHFEREALPQLAAKF
                     YAQAEEEKAHALKIAHYVTEAGGMLEIPAIPAGRARFESAEEAVQLALDWELAVTRQI
                     NDLVGLAMKESDHLAQLFLQWFVNEQLEEVTSMDMLLRTIKRAGDNLLLVEDFVARHT
                     QKGGSAGGGEAV"
     gene            complement(2475..4622)
                     /locus_tag="AC162G_00595"
     CDS             complement(2475..4622)
                     /locus_tag="AC162G_00595"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase family protein"
                     /protein_id="HIR5074344.1"
                     /translation="MKNRRAVFDLFLVSALGLFVELIFIRWAASELRVLAFYKNFALI
                     AAFLGLGLGFAVRRNDPAKGRFGRYFFPLLAVSVVVVLLLGRTPLSEAVLLNRATSQE
                     FIWAGTLGVSTPLVSAFLDVAFYVVLLMLFVLITVLFIPLGNLTARKFAAFRPLPGYT
                     INVLGSLAGILSYTLISFLGWPPAVWFLLVGAAGLYFLPRDLGRRWIVQAILAAVPVV
                     LALAWPTGADRTLWSPYYRIDITAQSAPSDPRLRLGYELAVNQAWHQSLWDLGPAFVA
                     DHYAAAPTHFDVLLAQYDTPQAVAPRLDRVLVVGAGTGNDVAGALRAGARHVTAVEID
                     PLILRIGEELHPEGPYADPGRVTLVNQDARSFFRHDSGQYDLIVFGLLDSHILFSTAS
                     SVRLDNFVYTQESLAEARDLLADDGLLALSFGVPRANEWVGLRIYRTLTDVFGHPPQV
                     YEFPSDDILFLIARDPLPAPLVRDPRVTPRPDYAYREDLGSVTDDWPYLYLQQHSVPT
                     TYLVGLCGAILMSVLLIRRALPNFRQIDLHFFFMGAAFFLLETKSITEMALLFGSTWV
                     VNAAVIAAILTMIVIANLVVERFHLTDARPYYVLLVAALLFDYFVPVSSFLGLPLAWR
                     LVLVSLAQAIPLFFAGMIFAITFSQTDSIEIALGSNLLGGVLGGIVEYASLALGIRSL
                     YLLALVFYMLSAMALRRVRVRRPSTVPDASLRT"
     gene            4843..6219
                     /locus_tag="AC162G_00600"
     CDS             4843..6219
                     /locus_tag="AC162G_00600"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5074345.1"
                     /translation="MPFLLRLNATLRSVLRRLLLLDRPVAPLSDAEVAAEVERHYRWN
                     FSVNLMDVAWFFFGVSFISASTLVPLFISKLTDSPLPVGFAAVIAQSAWFLPQLFTAN
                     AVERLARKKPVVVNLGLFLERVPIWVLVLAALTAGQSGGLALGLFLIGYAWHGLGAGV
                     VATAWQDMIARCFPVDRRGRFFGVSMFIGTSMGTMGAALSAQLLERFPFPTNFVYTFL
                     IAATGITVSWFFLSLTREPAQPVSTPRQSQREFWAQIPHIPRRDHNFRRFLIARLLLA
                     MGGMGAGFVTVAAVRQWQVPDSMVGMFTAAMLVGQTLGNLIFGLLADRFGHKLSLEFG
                     ALASLAAFALAWQAPSAEWYFAVFALLGITLGSIIVSGILVVMEFGEPSRRPTYVGMV
                     NTGVGLVGLVAPLLGAGLADVDYDLLFALSAVINLAALVVMRWWVKEPRFVRPAVALP
                     DATAQQRN"
BASE COUNT         1253 a         2007 c         1958 g         1111 t
ORIGIN      
        1 cgcatcgaaa aggcgcgcac gctgatccgc cccggtattc ccggcccggg cggcgtgtgg
       61 gccgacctgg gctgcggcga cggcattttc acggccgcgc ttcacacgct gacgcgacct
      121 gccgccgaga tattcggcgt cgaccgagat ctacgagcgc tagatgcgct ggcgcggcat
      181 ttcgcagagg gcttccctga agcccgaatt cgtcccatgc gggctgactt cacacagccg
      241 ctgacactcc caccgctcga cggcatcctc ctggcgaacg cgctccattt cgcccgggac
      301 aagcaggcgg cgttgaaaca cgccgccgcc ctgctgaagc cgggcggccg gttgatcgtc
      361 gtggaatata acaccgcgcg tggcaacccg gcggttcctt atccgctcga tgagatgggc
      421 ttcctcgaac tcacccgtgc gattgggctg cgcgaaggtc gtatcctggc gaggattcca
      481 tccacctttt tgggcgagat gtatgcaggc ataggcgttg cctgaatccc attcactact
      541 ccggcatttt tgccaaagtc cagttcactg caagggcacc ggcggctgcc acacttcgct
      601 cgtatcgttg acgcgaatcc ggctcaccca ctttacccaa tcgaaaccgc gatgatcgac
      661 cgccacgagc cgcagcggga agccatgccc gtgatcgaga gggcgcccgg ccacgtgtgt
      721 ggccaacaca tacccccgcg cctgctcgat tgagaaacgt cgcccgtatc ccgttatgcc
      781 ttcgacggtg aggctgcgag caccaggctt cacgccggcc aggttcagta attgggcaat
      841 agtaattcct tgccacactt gagtagaata ccagcctccg gtgcaatcga gcgtcgccgt
      901 ggcctcctcg tgggcaagcg gcccgagctg cgcataggtc agcgccagag ttcgttcgac
      961 aaagccgtcg acgatcaggc gccaatgctg gacctccaca ggcagcggat tgtcgagcag
     1021 ccaggccgta accggaaaca cgcctgtgaa gctgccggtc tcgtacgaac cagtgaatcg
     1081 ccggcgcgag ccgggcagtg cggccctcgc gacggtgctt tcgcccagtc gccacagcag
     1141 cacgccgctc accccggcgc cggccagccg caagaacgcg cgccggttca ccgccgaggg
     1201 aatgcgaaag acgaatcgca tgtacagcgt atgccacgcc agcagcccaa cacacgcgat
     1261 ggcaagaatc gcgtgtatcg ttatcagact gaatcccccg agagtcgtcc gcccggggca
     1321 gcgccgggcg ccgccgacgc acccacgcgt gcagaatgac cgcccctttc cagatcagga
     1381 gaacgacgat cgcgtagccg ccgatgccgt gcagccacag cacccagcgg aagtcttccg
     1441 caccgctcac caggccgcca acaccggtga ccaactgccc gagcagcaag acgagcagaa
     1501 cggtgttaac ccacggatac cgcatctacc caccaccggc ggagactgct cagaaggtcc
     1561 gaagttaacc gcttcacgcc catagtggcc ggtgctcgcg gccccttcgg gtgatccatc
     1621 caccgcccct gaactgccgg acggatcgcg cgaagcggag cgggagcgag ttagacagcc
     1681 tctccgcccc ctgcactgcc gcccttctgc gtgtgtcggg cgacgaagtc ttccaccagc
     1741 agcaggttgt cgccggcgcg cttgatcgtg cgcagcagca tatccatgct ggtgacttct
     1801 tcgagctgct cgttcacgaa ccactgcagg aagagctgcg ccaggtggtc cgattctttc
     1861 atcgccaggc cgaccagatc attgatctgc cgggtcacgg cgagttccca atccagcgca
     1921 agctgcacgg cttcctcggc ggactcgaag cgggcccggc cggctggaat ggcggggatt
     1981 tccagcatgc cgcctgcctc tgtcacgtag tgcgcgattt tcaatgcgtg cgccttttct
     2041 tcttcggctt gcgcataaaa cttcgcggca agctgcggca gcgcctcgcg ctcgaagtgt
     2101 gccgccagca tcacgtactg gagcgacgcg ccgaactcgt tgccgacctg ttggttgaac
     2161 gcctttgcga gattttcact gatgagcata gaagcctcct caatcgtggt tttcaggtgt
     2221 gggactcgag atgccacgca gggtaacgga acagcacaat gataccccaa tttgggccaa
     2281 tccggcacgt gatgaaccgc acggaactga tggacggcag ttgcgctgcc gtccgccctg
     2341 gcagcagtcc aactgcggtg gaaactgcgt gtggaatccg ccggatttcc atctggcggt
     2401 ttgagggcgg cggattgctc acccgaaggg gctgcgagca ccgcctgatt ttcgcacaga
     2461 gtgcgaaatc acggttaagt gcgcaggcta gcgtcgggga ccgtcgacgg acgccggacg
     2521 cgaacgcgcc tcagcgccat cgccgacagc atgtagaaga caagcgccag gagataaagg
     2581 ctccggatgc ccagcgccag cgaggcgtac tcgacgattc cacccagcac cccacccagc
     2641 aagttcgagc cgagcgcgat ctcgatcgaa tccgtctggc tgaaggtgat cgcgaagatc
     2701 atcccggcaa aaaacagtgg gatagcctgc gccaggctca ccagcacaag ccgccaggcg
     2761 agaggcaacc ccagaaaact gctgaccggc acgaagtagt caaacaatag tgcggccacc
     2821 agaaggacgt aatagggccg ggcatcggtc aggtggaaac gctcgacgac cagattggcg
     2881 atgacgatca tcgtcagaat cgcggcgatg acggccgcgt tgacgaccca cgtcgagccg
     2941 aacaggagcg ccatctcggt gatgctcttg gtctcgagca ggaagaaagc agcgcccatg
     3001 aagaagaagt gaagatcgat ctgtcggaag ttgggcagcg cccggcggat cagaagcacg
     3061 ctcatcagga tagcgccgca cagtccgacc aggtacgtcg tcggcacact gtgctgctgc
     3121 agatagaggt agggccagtc atcggtgacg gatccgagat cctcgcgata ggcgtaatcg
     3181 gggcgcggcg tgacgcgcgg atcgcggacg agaggagcgg gcagcgggtc acgcgcaatc
     3241 aggaacagga tgtcgtcgct gggaaattcg tagacctggg gtgggtgccc gaatacatcc
     3301 gtgagcgtgc ggtagatgcg cagtccaacc cactcgttcg cccgaggcac gccgaacgac
     3361 agcgccagta gcccgtcatc ggccagcagg tcacgcgctt cggccaggct ttcctgtgtg
     3421 tacacgaagt tatcgagccg cacactgctc gccgtgctga acaaaatgtg cgagtccagc
     3481 aagccgaaca cgatcaaatc gtactggccc gaatcgtgcc ggaagaaaga ccgcgcatcc
     3541 tggttcacca gtgtgacgcg cccgggatcg gcatacggcc cttcgggatg cagctcttcg
     3601 ccaatgcgca ggataagcgg atcgatctcg acggccgtga cgtgcctggc accggcgcgc
     3661 agcgccccgg cgacgtcgtt gcccgtgccc gctccgacaa ccagcacccg atccaggcgg
     3721 ggcgccacgg cctggggcgt gtcgtattga gcgagcagca catcgaagtg cgttggcgcg
     3781 gccgcgtaat gatcagccac gaaggccgga cccaggtccc acaggctctg gtgccatgct
     3841 tgattgaccg cgagttcata gccaagccgc agcctgggat cggatggagc agattgggca
     3901 gtgatgtcga ttcgataata cggcgaccac agcgtgcgat cggcgccggt cggccaggca
     3961 agcgccagta cgaccggcac ggccgctaga atggcctgga cgatccagcg gcgcccgaga
     4021 tcgcgtggca ggaaatacag ccccgccgcg ccgaccagca agaaccacac cgccggcggc
     4081 catcccagaa aagagatcag cgtgtacgat agaattccgg ccaggctgcc gaggacgttg
     4141 atcgtgtatc ccggcagagg ccgaaatgca gcgaacttgc gagccgtgag attgccaagc
     4201 ggtataaaga gcacggtgat caacacgaac agcatcaaca gcacgacgta gaaggccacg
     4261 tcgaggaaag cggacacgag cggcgtcgag acgccgagcg tccccgccca gatgaattcc
     4321 tgagaggtgg cgcggttgag gagaacggcc tcgctcaagg gggtgcggcc cagaagaagc
     4381 acgacgacga cgctgacggc cagcagcgga aagaagtacc gcccgaaccg gcccttcgcc
     4441 gggtcgttcc gtctcacggc aaatcccagc cccaacccga ggaacgccgc gatcagcgcg
     4501 aaattcttgt agaaggccag cacgcgaagc tcggaggccg cccaacgaat gaagatcagc
     4561 tcgacgaaca ggccgagcgc gctcaccagg aacaggtcga agactgcacg ccgattcttc
     4621 atgcgcaaca ctccttcaca gcaggctcgg tgaagaaatt cgcgctgagt atagcatgat
     4681 gcatacgggc tgataggttg caattctatt acaagttgcg gcggcaggct acgttgtgct
     4741 atagtgttaa cgcaggccgc gaaatttctt cacggggaga agtcagatgg aagccttctc
     4801 cagttcgtcc aaccattgta ctgccggacg ccagagagct ctatgccatt ccttctgcga
     4861 ctcaatgcga ccttgcgctc agtcctgcgc cggcttctgt tgctcgaccg gccggtagcc
     4921 ccgctcagcg acgccgaagt cgccgccgag gtcgagcgcc attaccgctg gaatttctcc
     4981 gtcaacctca tggatgttgc ctggttcttc ttcggcgtga gtttcatctc cgccagcaca
     5041 ctcgttccgc tgttcatcag caaattgacc gatagcccgc tgccggtggg cttcgccgcc
     5101 gtcatcgccc agagcgcctg gtttctgccg cagttgttca ccgccaatgc cgtcgagcgt
     5161 ctggcgcgca agaaacccgt cgtcgtcaac ctgggcttgt tcctcgaaag agtgccgata
     5221 tgggtattgg tgctcgccgc gttgacagcc ggtcaatccg gcggcctggc gttggggctg
     5281 ttcctcatcg gatatgcctg gcacggactg ggcgcgggag tggtcgccac cgcctggcaa
     5341 gacatgatcg cccgctgctt tccggttgat cggcgcgggc ggttcttcgg agtcagcatg
     5401 tttatcggaa ccagcatggg cacgatgggc gcggcgctca gcgcccaact gctggagaga
     5461 ttccccttcc ccacgaattt tgtctacacc ttcttgatcg ccgcgaccgg cattaccgtc
     5521 agttggttct ttctgtccct gacgcgcgag ccggcgcagc ctgtcagcac ccccagacaa
     5581 agccaacggg aattctgggc gcagatcccc cacattccgc gccgcgatca caacttccgg
     5641 cgcttcctga ttgcgcgact gctgctggcg atgggcggga tgggcgccgg cttcgtcacc
     5701 gtggccgccg tgcggcaatg gcaggttcca gacagcatgg tgggcatgtt taccgcagcg
     5761 atgttggtgg ggcaaacact gggcaacctg atctttgggc tgctggccga tcggtttggc
     5821 cacaagctgt cgcttgagtt tggcgcgctg gcatcgcttg ccgctttcgc gctggcctgg
     5881 caggcaccgt cggccgagtg gtacttcgca gtcttcgccc tgctggggat cacactcggc
     5941 tcgatcatcg tttccggcat tctggtggtg atggagttcg gagagccgtc acgccgaccg
     6001 acctacgtcg gcatggtgaa taccggcgtg ggattggtcg gcctggtcgc gccgctgctc
     6061 ggcgccgggt tggccgatgt cgactatgac ctgctcttcg ctctgagtgc agtcatcaat
     6121 ctggcggcgc tggttgtgat gcgttggtgg gtgaaagagc cgcgctttgt tcgcccggcc
     6181 gttgcgctgc cggacgccac ggcacagcag cgcaactgat gtggcaacaa cgacacgttt
     6241 gttcgtccgg cagttgcact gccgggcgcc acggcacagc agcacaactg atgtgccaac
     6301 agcgactgga gtgtgctata gttacagtc
//
LOCUS       DBNHXB010000017         4702 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2855733, whole genome shotgun sequence.
ACCESSION   DBNHXB010000017 DBNHXB010000000
VERSION     DBNHXB010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2855733"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="AC162G_00605"
     CDS             <1..1244
                     /locus_tag="AC162G_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR5074346.1"
                     /translation="QTKLFAKSKTLYGLHFARNAIRESGRAVIVEGYMDAIAAHQAGF
                     ANVVASLGTALTEMQFRQLQKLAPHFILALDADAAGVNAMLRGLDVARETLDRDLQPM
                     FNPRGLIGFEGKLKVDIRVLALPPEMDPDDLIRSAPQKWSELVDGAKPVVQFVTDTLA
                     AGRDLGDPKEKAALSREVAPFIRDVADPVERAAYAQRLARLLKIDERAVLDQVGLAGA
                     SASRRAKTSEPVQPPTPARAALDRERYCLAELLRATSPKETLHAIDDTLTRVDLPPFG
                     AEDFESVTHREVFLAIHSALEEIGSLSLDDVLDRLDPSLHADVPAWLESPPPRPQGGP
                     DVDETRGVVDAALLLRERNLRTQGAQIEDLIRSASEEGDAESVRELGQAKLVLSDQMR
                     RLSRIRYAPEFIRQSYADGGR"
     gene            1342..2934
                     /gene="rpoD"
                     /locus_tag="AC162G_00610"
     CDS             1342..2934
                     /gene="rpoD"
                     /locus_tag="AC162G_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR5074347.1"
                     /translation="MPKRPKIAPRLSEPISDDLALDETEPIEVDLATGEVLLGEEETF
                     VEDEPAEADLEEIEFEEEEEDEEREPLEWSAELADDPVRMYLKEIGQVRLLDVDQEVW
                     FATQMNAVEHLHQIEDRARERLHREPAETDVMREIYANLQASWKGVEKASRKTHARKP
                     NLAALASEVRTLRTSPSMTNPSTLRPYIGNGRVERVAGWTELTGMLFDVYALLYLMPI
                     ETLDWLFERLRKKRTLPDTRSFLKALPDAQRIRESFQILAEYSERAKQSLIRANLRLV
                     VSVAKRYIGRGISFLDLIQEGNIGLLRAVEKFDHTRGYKFSTYATWWIRQAISRAIAD
                     QARTIRIPVHMVETINRLLRAQRELMQELGREATAEEIAVELGLLEPDETQAIQAAWA
                     TNQPLDPALDRKVRRAAGKVRRIVRISQEPMSLEMPVGQEESSMLSDFIEDETVVGPV
                     DAASNQLLKEQVQSSLDALSEREREVLEMRFGLRDGQSHTLEEVGQFFGVTRERIRQI
                     EAKALRKLRHPYRSRKLRDYLT"
     gene            complement(2974..3801)
                     /locus_tag="AC162G_00615"
     CDS             complement(2974..3801)
                     /locus_tag="AC162G_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vacuolar protein sorting-associated family 26
                     protein"
                     /protein_id="HIR5074348.1"
                     /translation="MSALHIELDAAEAIYRPGDTLRGRLVLTLPSEQHPRSITLHAVG
                     TEFTNWGTQPSYIARTHPLDQAIEVWKAASESSALPAGTHAFPFEVVLPPHLPPSFDG
                     MLTEIGYGIRAKVDLPRHVDLHAEAGFVVLASVPAVVEPPAPVEAHDASGRKIKLALP
                     RSVYRLGETVEGAARVTRAGEGRARRLTIELLSRERGGAQGIWAEDVEQEAESRVELE
                     HVAGDMTYPFTFKAPDWAVPTFRGEHSALTWHVRARLEVARMPDLIAEAEITIVEPS"
     gene            complement(3798..4325)
                     /locus_tag="AC162G_00620"
     CDS             complement(3798..4325)
                     /locus_tag="AC162G_00620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR5074349.1"
                     /translation="MDAYKTIVSLRAVRQFQDTPIPDDALHRILQAGRMSGSSKNTQP
                     WWFVVIRDAETRLRLSKCGDFASHLIGAPLAIGMVLDPQYYRGEFDSGRAVQNMMLAA
                     WASGIGSCIASMHREEDAKAVLGVPAEYRMQHVLSFGYPVPQPGDTIEGKPRASVLKR
                     TGRKPMEDIVRHERW"
     gene            complement(4412..>4702)
                     /locus_tag="AC162G_00625"
     CDS             complement(4412..>4702)
                     /locus_tag="AC162G_00625"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR5074350.1"
                     /translation="REMIERGEAPFDLIFVDADKKAYVEYLNLSIRLAHPGTVMLADN
                     VIRRVMSPAPGDEDSRGIHAYNEAIAAHPRLESLILPIIRENLDGLSISIVR"
BASE COUNT          853 a         1532 c         1550 g          767 t
ORIGIN      
        1 cgcagaccaa gctcttcgct aagagcaaga cgctgtacgg cctgcatttt gccaggaacg
       61 cgattcgcga atcgggcaga gccgtgatcg tcgaaggata catggacgcc atcgccgcgc
      121 accaggcggg gttcgcgaac gtcgtcgcct cgcttggcac ggcattgacc gagatgcagt
      181 tcaggcaatt gcagaagctg gcgccgcact tcattctcgc gctcgacgcg gacgcagccg
      241 gcgtcaacgc catgctgcgc gggcttgacg tggcgcgaga aacgctcgac cgggacctgc
      301 agccgatgtt caatccgcgc ggcttgatcg gcttcgaggg caagctgaag gttgacatcc
      361 gcgtgttggc gctgccccca gagatggatc cggacgatct catccgctcg gcgcctcaga
      421 agtggagcga actggtcgac ggcgcaaagc cggtcgtgca gttcgtgacg gacacgctgg
      481 ccgctggacg cgatctgggc gacccgaagg agaaggcggc gctgtcgcgc gaagtcgcgc
      541 cgttcattcg cgacgtcgcc gatccggtcg agcgagcggc gtacgcgcag cgcctggcgc
      601 gactgctgaa aatcgacgag cgcgcggtgc tggatcaggt cgggctggcc ggcgcgtctg
      661 catcgaggcg ggctaaaacg tccgagccgg tccagccgcc aacgcccgcg cgggcggcgc
      721 tcgaccgcga gcgttactgc ctggccgaat tgctgcgtgc gacatctccg aaggagacgc
      781 tccacgcgat cgacgatacc ttgacgcgcg tcgacctgcc gccgttcggc gccgaggact
      841 tcgaaagcgt cacacaccgt gaggtcttcc tggcgattca ttcggcgctg gaagagatcg
      901 gcagcctatc gctcgacgac gtgctcgatc ggctcgatcc ttcgctgcac gccgacgtgc
      961 cggcctggct ggaatcgccg ccgccacggc ctcaaggggg tccggacgtc gacgagacac
     1021 gcggcgtcgt ggatgcggcg ctgctgctgc gcgagcggaa cttgaggacg cagggcgcgc
     1081 agatcgaaga cttgatccgc agcgcctccg aagaaggcga cgcggagagc gtccgcgagc
     1141 tgggtcaggc caagctggtc ctgtccgatc agatgcggcg cctgtcacgc atccgatatg
     1201 cgccggaatt catccgccag tcttacgccg acggcgggcg ctgaccctac ttgccacaat
     1261 cggacgacgc gcgtagaata gtgcgccgta cacgcctggt tcagcatagg cgattcgaat
     1321 cagccgttgc gcgaccgatc tatgcccaag cgaccgaaga tagccccgcg acttagcgaa
     1381 ccgattagcg acgacctggc cttggacgag accgaaccca tcgaggtcga cctggcgacc
     1441 ggcgaagtct tgctcggcga ggaggagacg ttcgtcgagg acgagccggc ggaggccgat
     1501 ctcgaggaga tcgagttcga ggaagaagag gaagacgagg aacgcgagcc gctcgagtgg
     1561 agcgctgagc tggccgatga tcccgtgcgc atgtacctca aggagatcgg ccaggtgcgt
     1621 ctcctcgacg tcgatcagga ggtgtggttc gccacgcaga tgaacgcggt cgagcacctg
     1681 caccagatcg aagatcgcgc acgcgagaga ctgcaccgcg agccagccga gaccgacgtg
     1741 atgcgcgaga tctacgccaa cctgcaggct agctggaaag gcgtcgagaa ggccagccgc
     1801 aagacgcacg ccaggaaacc gaatctggcc gcgctggcca gcgaggtgcg cacgctgcgc
     1861 accagcccga gcatgaccaa cccttccacg ctgaggccgt acatcggcaa cgggcgcgtc
     1921 gagcgcgtcg ccggctggac cgagctgacc ggcatgctgt tcgacgtgta cgccttgctg
     1981 tatctcatgc cgatcgagac gctcgattgg cttttcgagc gtttgaggaa gaagaggacg
     2041 ctgcccgaca cgcgctcgtt cctgaaggcc ctgcccgatg cgcagcgcat ccgcgaatcg
     2101 tttcagattc tggccgagta ttcggagcgc gccaagcaga gcttgatacg cgccaatctg
     2161 cggctggtcg tcagcgtcgc caagcgctac atcgggcgcg gcatttcgtt cctggacctg
     2221 atccaggaag gcaacatcgg cttgctgcgc gctgtcgaga agttcgatca cacacgcggc
     2281 tacaagttca gcacgtacgc cacgtggtgg atccgccagg cgatcagccg cgccatcgcc
     2341 gaccaggccc gcaccatccg catccccgtt cacatggtcg agacgatcaa ccgtctgctg
     2401 cgcgcgcagc gtgagctgat gcaggagctg ggtcgcgaag cgacggccga ggaaatcgcc
     2461 gtcgagctgg gcctgctcga gccggacgag acacaggcga tccaggcggc gtgggcgacc
     2521 aaccagccgc tcgacccggc gttggatcgc aaagtgcgcc gcgccgccgg caaggtgcgc
     2581 cgcatcgtgc gcatcagcca ggagccgatg tcgctggaga tgcccgtggg ccaggaagaa
     2641 tcgagcatgc tcagcgactt catcgaggac gagaccgtcg tcggcccggt cgacgccgcg
     2701 tcgaaccagt tgctcaaaga gcaggtgcag tcgagcctcg acgcgctatc cgagcgcgag
     2761 cgcgaggtgc tcgagatgcg tttcggcttg cgcgacggcc agagccacac gctcgaggaa
     2821 gtcggccagt tcttcggcgt cacccgcgag cgcatccgcc agatcgaagc caaggcgctc
     2881 cgcaagctgc gccaccccta ccgcagccgc aagctgcgcg attacctgac gtgattagac
     2941 ctggcaggtc tcgaagacct gccaggtcta tcgtcaggac ggctcgacga tggtgatttc
     3001 cgcctcggcg atcagatcgg gcatgcgcgc cacctcgagc cgcgcgcgga cgtgccacgt
     3061 cagcgcggaa tgctctcccc gaaaagtggg cacggcccaa tcgggcgctt tgaacgtgaa
     3121 cggatacgtc atgtctcctg cgacgtgttc cagctcgacg cgcgattcgg cctcctgctc
     3181 gacgtcctcg gcccaaatgc cttgtgcgcc gccgcgctcg cgtgaaagca gctcgatcgt
     3241 caaacgccgc gctcgcccct cgcccgcacg ggtgacgcgc gccgcgccct cgacggtctc
     3301 gcccaagcgg tacaccgagc ggggcaacgc caacttgatc ttgcggcccg atgcatcgtg
     3361 cgcctcgacc ggcgcgggcg gctcgacgac ggccggcacg cttgccagga cgacgaaccc
     3421 tgcctcggcg tgcaggtcga cgtggcgagg caaatccact ttcgcgcgaa tgccgtagcc
     3481 gatctccgtc agcatgccgt cgaagctggg cggcaggtgc ggcggcagga cgacctcgaa
     3541 cgggaacgca tgcgtcccgg ccggcagcgc gctgctctcc gaggcggcct tccagacctc
     3601 gatcgcctga tccagcgggt gcgtgcgcgc gatgtacgat ggctgcgtcc cccagttcgt
     3661 gaactcggtg ccgacggcgt ggagcgtgat gctgcgcgga tgctgctcgc tcggcagggt
     3721 caagaccagc cggccgcgca gcgtgtcgcc gggacgatag atggcctcgg cggcatccag
     3781 ttcgatgtga agtgcgctca ccatctctcg tggcggacga tgtcttccat cggcttgcgg
     3841 ccggtgcgct tgagcacact cgcgcgcggc ttgccttcga tcgtgtcgcc cggctgcggg
     3901 accggatacc cgaatgagag cacgtgctgc atccggtatt ccgccggcac gccgagcacg
     3961 gccttggcgt cttcttcgcg gtgcatcgac gcgatgcacg agccgatccc gctcgcccag
     4021 gcggccagca tcatgttctg aacggcgcgg cccgaatcga actcgccgcg atagtactgc
     4081 ggatcgagca ccatcccgat cgccagcggc gcgccgatca ggtggctggc gaagtcgccg
     4141 cacttcgaga gccgcaggcg tgtctccgcg tctcgaatga cgacgaacca ccagggctgc
     4201 gtgttcttgc tgctgccgct catgcgcccg gcctgcaaga tgcggtgcag cgcgtcgtcc
     4261 gggatgggcg tgtcttgaaa ctggcgcacc gctcgcagtg aaacgatcgt cttgtacgcg
     4321 tccatgtccc tctccatctg tgtatctatc tcgacagtgt cagaatcacg tgattacgtg
     4381 atcacgtgat cacgtatcac gtaattactc gtcatcggac aatcgagatg gataggccat
     4441 ccagattctc ccggatgatc ggcaatatca gagactccag gcgcgggtgg gcggcgatcg
     4501 cctcgttgta cgcatgaatg ccgcgcgaat cctcgtcgcc gggagcgggc gacatcacgc
     4561 gccggatcac gttgtcggcc agcatgactg tgccgggatg cgcgagccgt atcgagagat
     4621 tcaggtactc gacgtacgcc ttcttgtcgg cgtcgacgaa gatcaagtcg aaaggcgcct
     4681 caccgcgctc gatcatctcg cg
//
LOCUS       DBNHXB010000018         3638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4370, whole genome shotgun sequence.
ACCESSION   DBNHXB010000018 DBNHXB010000000
VERSION     DBNHXB010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4370"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="AC162G_00630"
     CDS             complement(<1..724)
                     /locus_tag="AC162G_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR5074351.1"
                     /translation="MPYEAILTEAKDRVGLIRLNRPQQLNALNAQQMEEVATALEEFD
                     RDENVGCIVITGNERAFAAGADIKEMAGASAADMLTHDAISRWDRIKKIKKPIVAAVS
                     GWCLGGGCEVAMACDIIVASETAQFGQPEINLGVMPGAGATQRLTRAIGKSKAMEMVL
                     TGRTLSAREAEQAGLASRVAPVEVYLDEALKLAGEIASRAPIAIRLAKEAVNKSYEMP
                     LADGLDYERRLFYFLFATEDQKE"
     gene            complement(743..2188)
                     /locus_tag="AC162G_00635"
     CDS             complement(743..2188)
                     /locus_tag="AC162G_00635"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5074352.1"
                     /translation="MNLKGQTLGDYHLESVIGRGPRATVYRARHVADSRIVAVKVYDE
                     SIDPDRARQAVESTQALTGAHVLPVEGFGVHHGLAYVAMRYMPKGSLKARWRRAVPMA
                     DIARILPQIASALDHAHGLGVWHLNLKPGNVLLDRPGNAFVADFGIPPLPDSPYTAPE
                     IGRGSGGDARADVYALGAVLYEMLTGRAPLARRPRDELSNQRMADLPSPRSIRPGIPT
                     AVEAVTMRALSIDPESRYAAPGALAQAFAEASEAAAGEQLLQEPSRRRPPLGWIAGSV
                     VLLLLVAVVVLSGGLNAPAAAPAPSPTLTATEVVTATPSPIPTLTNTYTPTAKRATAT
                     ATNRARTPEPTRMSEPTASATGIPAPVTGFTQTPTFRVVSLALKPPANRPGPPDRLDL
                     YFDAVIEPAAGGPFGQLFAYLPQIDSLVSSRIGAQVSSGTQLLHVALVVDCLQLPEPF
                     TTNQVFLEIRETDRSPALYAQTIDYTKTWCR"
     gene            complement(2210..>3638)
                     /locus_tag="AC162G_00640"
     CDS             complement(2210..>3638)
                     /locus_tag="AC162G_00640"
                     /inference="COORDINATES: protein motif:HMM:NF015755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR5074353.1"
                     /translation="CMSYDVEARPSRSRVGLWIAILAVFLILGGLASIAAFNAVRDFV
                     RDLPLDLPSQPAAPAGTPGAASPIVVPEWDGVERVNILLLGIDEREVEAGPWRTDTMI
                     LLTIDPATKSAGMLSIPRDLWVPIYGYDIEGKINTAHFIGDAQDYPGGGPALAIATVQ
                     YNLGIPVHNYIRLNFSAFERLIDLIGGVEICVDQPIDDPQYPDSGFGYEPLHIDAGCQ
                     VMDGRLALKYARTRHTEFGDFDRAHRQQQVILAVRNKVARADMLPSLVAQAGPLLETL
                     GDSVKTDFEPDQLIRLASLASKIDLDKIQSLVIEPSMTLSYMAPTDPPQAVLIPIREE
                     IRKLRDRLLDVGGVTAPEAADDPARLAAEAAAIRVENGTQTPGLAARTRDFLSERGFV
                     IADVGDAFDGLTDHAATLIIDHGNKPFTAAKLAEALGLPLVAIRTAATNGDGVDVRIV
                     LGSDFASTLGAQLLLTPAPTATAAP"
BASE COUNT          578 a         1175 c         1250 g          635 t
ORIGIN      
        1 cttccttctg gtcttcggtc gcgaacagga aatagaacag gcggcgctcg tagtcgaggc
       61 cgtcggccag cggcatttcg tatgacttgt tgacggcctc tttcgccagc cgaatcgcga
      121 tcggcgctct cgacgcgatc tcgccggcga gcttgagcgc ctcgtccaga tacacttcga
      181 cgggcgcgac gcggctggcc agcccggctt gctcggcctc gcgcgccgac agggtgcgcc
      241 cggtgagcac catctccatc gccttgctct taccgatggc gcgcgtcagg cgctgcgtcg
      301 cccctgcgcc cggcatcacg ccgaggttga tttccggctg gccgaactgc gccgtttccg
      361 aggcgacgat aatgtcgcag gccatcgcca cttcgcagcc gccgcccagg caccaccctg
      421 acacggcggc gacgattggc ttcttgatct tcttgatgcg atcccagcgg ctgatagcgt
      481 cgtgcgttag catatcggcc gccgacgcgc ctgccatctc cttgatgtcg gccccggcgg
      541 cgaaagcgcg ctcgttgccg gtgatcacga tgcagccgac gttctcgtcg cggtcgaact
      601 cctccagcgc cgtcgcgact tcctccatct gttgggcgtt cagcgcgttg agctgctgcg
      661 gccggttgag gcgaatcagg ccgacgcggt ctttggcctc ggtcaggata gcttcgtagg
      721 gcatagtgct cctttgatcc gactaacggc accacgtctt cgtgtaatcg atcgtctgcg
      781 catacagcgc cgggctgcgg tcggtttccc gtatctccag gaacacctgg ttcgtcgtga
      841 acggctctgg caattgtaag cagtcgacga cgagcgccac gtgcaacaac tgcgtccccg
      901 acgacacctg cgcgccgatc cgcgacgaca ccagcgaatc gatctgcggc aggtacgcaa
      961 acagttggcc gaacggcccg ccggctgccg gctcgatcac agcgtcaaag tataagtcca
     1021 aacggtctgg aggtcctggc cgattggcgg gcggtttcag tgcaagtgac acgacacgga
     1081 acgttggcgt ttgcgtgaac ccggtaacgg gggccggtat gccggtggcc gatgcagtcg
     1141 gctctgacat gcgtgtcggt tccggtgtcc gcgcgcggtt agtcgcggtg gctgttgcgc
     1201 gtttcgctgt gggcgtatag gtattcgtga gcgttggaat gggtgatggc gtcgcagtga
     1261 caacttccgt cgccgtgagg gtcggcgatg gcgcgggcgc ggcggcgggc gcgttcaatc
     1321 cgcccgagag cacgacgacg gcgacgagca gcaggagcac aacggagccg gcaatccatc
     1381 ccagcggcgg gcggcggcgt gacggttcct gcaataactg ctcgccggcc gccgcctccg
     1441 acgcctctgc aaacgcctgg gccagcgcgc cgggcgccgc atagcgcgac tccggatcga
     1501 tcgacagcgc gcgcatcgtc accgcttcga cggcagtcgg gatgcccggc cgaatcgatc
     1561 gcggcgaggg caggtcggcc atgcgctgat tggacaactc gtcacgcggg cggcgggcga
     1621 gcggcgcgcg cccggtcagc atctcgtata atacggcgcc gagcgcgtac acgtcggcgc
     1681 gcgcgtcgcc tccgctgccc cgcccgatct ccggcgcggt gtagggtgaa tcgggcagcg
     1741 gcgggatgcc gaagtcggcg acgaaggcat ttccgggccg gtcgagcagg acgttgcccg
     1801 gcttgagatt gaggtgccac acgccgagac cgtgcgcgtg atcgagcgcc gaggcgattt
     1861 gcggtagaat gcgagcgatg tcggccatcg gcacggcgcg tcgccagcgt gccttgagcg
     1921 agccttttgg catgtaacgc atggcgacgt aggccagccc gtgatgaacg ccgaaaccct
     1981 cgaccggcag gacgtgcgcc ccggtcagcg cctgcgtcga ttcgacggcc tgccgggcgc
     2041 ggtcgggatc gatcgattcg tcgtagacct tgacggcgac gatgcgactg tcggccacat
     2101 ggcgcgcgcg gtacaccgtg gcgcgtggcc cgcgcccgat gacgctttcc aggtggtaat
     2161 cgcccagcgt ctggccctta aggttcatgc agattccgta tcccgggcgt cacggcgccg
     2221 ctgtcgctgt tggcgctggc gtgagcagca actgcgcgcc gagcgtcgac gcaaaatcgc
     2281 tgcccagcac gatgcgcacg tcgacgccgt cgccgttggt cgcggccgtg cgaatcgcca
     2341 ccagcggcag gcccagcgct tcggccagtt ttgcggcagt gaacggctta ttgccgtgat
     2401 cgatgatcag cgtggcggcg tggtcggtca ggccgtcgaa ggcgtcgccg acgtcggcga
     2461 tgacgaatcc tctttcgctc agaaaatcgc gcgtgcgcgc cgccaggccg ggcgtctgcg
     2521 tgccgttttc gacgcggata gcggcggcct cggcggccag gcgcgccggg tcgtcagccg
     2581 cttccggcgc ggtgacgccg ccgacgtcga ggagacggtc gcgcagcttg cggatctctt
     2641 cgcggatcgg aatcagcact gcctgtggcg ggtcggtcgg agccatgtac gacagcgtca
     2701 tcgacggctc gatgaccagc gactgaatct tgtccagatc gatcttcgag gcgaggctcg
     2761 ccagccggat gagctggtct ggctcgaaat cggtcttgac cgagtcgccg agcgtttcga
     2821 gcagcggccc ggcttgcgcg accagcgacg gcagcatgtc cgcgcgcgcc accttgttgc
     2881 gcacggcgag gatgacctgc tgctggcggt gcgcgcggtc aaaatcgccg aactcggtgt
     2941 ggcgcgtgcg ggcgtatttc agggcgaggc ggccgtccat cacctggcag ccggcgtcga
     3001 tgtgcagcgg ctcgtagcca aacccggagt cgggatactg cggatcatcg atgggttgat
     3061 cgacgcagat ctcgacgccg ccgatcagat cgatcaggcg ctcgaacgcg ctgaagttga
     3121 gacggatgta attgtgaacc gggatgccaa gattatactg gacggtggct atggccagcg
     3181 ccggcccgcc gccggggtaa tcctgcgcgt cgccgatgaa atgcgccgtg ttgatcttgc
     3241 cttcgatgtc gtagccgtag atcggcaccc acaggtcgcg tgggatcgac agcatgcccg
     3301 ccgatttcgt cgccgggtca atcgtcagca ggatcatcgt gtcggtgcgc cacggtccgg
     3361 cctcgacctc gcgctcgtcg atgcccagca gaaggatgtt cacccgctcg acgccgtccc
     3421 actcgggcac gacgatcggg ctggctgcgc cgggcgtgcc ggccggggca gccggctgcg
     3481 acggcagatc gagcggcaga tcgcgcacga aatcgcgcac ggcgttgaac gcggcgatcg
     3541 acgccaggcc gcccaagatc aggaagacgg cgagaatggc aatccacaac ccgacccgcg
     3601 atcgcgacgg tcgagcttcg acatcatacg acatgcag
//
LOCUS       DBNHXB010000019        16820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4365981, whole genome shotgun sequence.
ACCESSION   DBNHXB010000019 DBNHXB010000000
VERSION     DBNHXB010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 16820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16820
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4365981"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..990
                     /locus_tag="AC162G_00645"
     CDS             115..990
                     /locus_tag="AC162G_00645"
                     /inference="COORDINATES: protein motif:HMM:NF026954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR5074354.1"
                     /translation="MTRVRRALLYMPGDDMRKIGKAATLDVDCVCLDLEDGTALNRKQ
                     AARETIVQALRTLDFGRSERLVRINPVGSGMEVDDLAATIDGRPDGYVLPKVESAEEV
                     EWLCWRLTGAEQARGWPLGSTNVLAIVETARGIINLRDISGCDRRLVALIFGAEDLAG
                     DLGATRTREGWEVFYARSAVVTHAAAFDLQVIDMVCIDFRDTEALIRESLEGARMGFT
                     GKQIIHPNQVEPVQAAFTPSDEAIAHARRIVEAFEAHQAEGAGAFALDGKMVDMPVVK
                     AAQKVLEKAKAAGKV"
     gene            1005..1814
                     /locus_tag="AC162G_00650"
     CDS             1005..1814
                     /locus_tag="AC162G_00650"
                     /inference="COORDINATES: protein motif:HMM:NF016816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HIR5074355.1"
                     /translation="MAADMEGISGVVHWDHVTPGHAEYARFRRIMTHDVNAAIAGAFD
                     AGAEEVAVTDGHRDNLNILVEELDPRARLNSGRPSPLSMVQDIGMDVSGVMFVGYHAR
                     AGAQNAILDHTWSDMCVANLWLNGRLTGEIGLNAAVCGHFDAPVIMISGDQTACAEAV
                     ETLGAVEVAVVKHATGRMAAECLPPEAAQRKIREAAGRAVNRLRMGRAASPYRPESPI
                     TVAIEFTKSEMADRAMLLSGARREGRRVELTTEDMPSAYRLFRAAVTLAKG"
     gene            1824..2447
                     /locus_tag="AC162G_00655"
     CDS             1824..2447
                     /locus_tag="AC162G_00655"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5074356.1"
                     /translation="MTKDQRRRPAGMQARADTPALEFYPLTPDRWRDLEALFGPRGAV
                     GGCWCMWWRIKRTEFERRQGDGNKRAFKKIVKSGEEPGILAYAGGQPIGWCAVAPREA
                     YPVLDRSPTLKRVDDRPVWSVTCFFIARPWRRQGVTVALLKAAIEYAARRGATIVEGY
                     PVAPKKDSMPEMYAFTGTASAFRKAGFVEVARRSETRPIMRYMLPIP"
     gene            2515..3435
                     /locus_tag="AC162G_00660"
     CDS             2515..3435
                     /locus_tag="AC162G_00660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5074357.1"
                     /translation="MGTSELNVVTGAFGYTGKYVARRLLSMGKRVKTLTTHPDRPSPF
                     GDRVSVAPFDFDRPEALIESLRGAAALYNTYWVRFDYGSATFEAAVENTKTLFRAAKE
                     AGVRRVVHVSVSNPSEDSPLSYFRGKALLEKALVESGLSHAIVRPTVTFGDEDILINN
                     IAWLLRRFPVFAILGEGDYRVQPIFVEDVAELMVSAGSQSGNLVVDAAGPEVYTFDTL
                     VRTIKSAVRSRARIVHLPPRLALFLSQVIGVFVGDVVLTQEEVDGLMAGLLVSSGPPT
                     GRTHLSEWLREHAHTVGAKYASEVTRHFRS"
     gene            3598..4581
                     /gene="trpS"
                     /locus_tag="AC162G_00665"
     CDS             3598..4581
                     /gene="trpS"
                     /locus_tag="AC162G_00665"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR5074358.1"
                     /translation="MAKKGRVFSGARPTGRQHLGNYLGAIQNYVALQADYECVYCIVD
                     LHALTTLEQTDDLKAYTREMVLDWLAAGIDPALGTIVFVQSHVPMVTELHWILSTITS
                     LGKLTDLPTFKEKVRQQPHNVNYALPGYAVLMAADIALYKTDVVPVGVDQESHLEFTR
                     EIVRTFNHRFKPVLVEPQMKLTEYPKILGIDGENKMSKSLDNHIELAATPEETQQRVM
                     MMVTDPQRTHRNIPGRPEVCNVYSLHRVFSPGQVDQIYTDCTTAGIGCVDCKKILARN
                     LNAHLEPFRARRAVLAAQPNLVDDVLQDGARKATAIAAQVLAEVKDAVGLP"
     gene            4581..5402
                     /gene="mutM"
                     /locus_tag="AC162G_00670"
     CDS             4581..5402
                     /gene="mutM"
                     /locus_tag="AC162G_00670"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIR5074359.1"
                     /translation="MPELPEVESYVRALRAPLIGRTITAVHVYWPRTVAHPSPREFTR
                     RLPGLRVNAIDRRAKYLVFALSAKSSQVPTDYLLIHLKMSGQLDVTSHPKPIDKHDRV
                     IFDLSGGRRLCFNDMRKFGRLYLVPHPEVVTGRLGPEPLSDEFTLDIFKRRLGKRKGR
                     LKSLLLDQTFVAGIGNIYADEALWLARLHPLRRADSLSDAETRALYRAIRAALREGIK
                     RNGASFDRVYRSGDYEFRVYGRADRPCRRCRQPIRRTVVGQRGTYFCATCQSLRG"
     gene            5413..6108
                     /locus_tag="AC162G_00675"
     CDS             5413..6108
                     /locus_tag="AC162G_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074360.1"
                     /translation="MEPEQLILIVGALLIGALLVLGGILAGLRGRRRSKSDRDSLPAA
                     ASEQVSKREPPSWRNEWQRKLPPDVVLLARDAATGEWVVEIEGQRYRRLSDVHDDKAA
                     TKIVSAIDGLKAFAGLASKASPESTAPQRSSVVSDPGSLVPPPTGPRRPKLATYSAPE
                     GSIIAQIEQILQLELGRRPDLANRSIHMGALPDGSLVVEVDGSFYRTPDEIPDAAARE
                     IVLRAVRMWETSS"
     gene            complement(6188..7312)
                     /gene="add"
                     /locus_tag="AC162G_00680"
     CDS             complement(6188..7312)
                     /gene="add"
                     /locus_tag="AC162G_00680"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIR5074361.1"
                     /translation="MNQVRRSDAAPLRLSDPSALPPSGVAGQALRHVVHSLPKADLHR
                     HLEGSLRLSTLVEIAQEHGVDLPSYNIEDLRPYVQITDDPANFHRFLEKFHLLRRFYK
                     TPEAVARVAYEAVADAAQDHVHYLELRFNPAALARTQGFSYEEVTDWVIEAVDRAQAD
                     YRITVRLIATAVRNEGVENAKRIAEVAIARKERGMVGLDLAGDEVNFDAAPFAPVFRE
                     AREAGLGITIHAGEAGGADNVQHAVLDMGATRIGHGVRAIENSDVIKLLRERHVTLEV
                     CPTSNLQTGVMHNFSHHPLRDLYMLGVAVTLNTDDPSVSDTTLTDEYLVALMTMGIRL
                     SHLRRMNVNALRAAFLPEAVRREMEQKVMAEFDRLLEGLT"
     gene            complement(7309..11265)
                     /locus_tag="AC162G_00685"
     CDS             complement(7309..11265)
                     /locus_tag="AC162G_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5074362.1"
                     /translation="MSDAPESPDRKNQLPLNPALSGVEGEAVSHAVDAGDPLRSTTRE
                     GGAARRPSGADRRSLTVLQEIALALNSAARMADVPELILTQLQRVLPYHSASIFLTED
                     GVLRSVVTRSPHGAPLVHATRDRDYTDLSPWREVVTTQRSILLSDTRGDPRWPGLERS
                     ALSRSPERSAGGVEGPGSVGCWLGVPLLVRDQLIGVLALEHVERAAYGEDDVRVAEVF
                     ATQAAIAIENARLLEEARRRADHMAALNKVAATVSQELDLEQTLRIALDKALDVMGVE
                     AGAISLIEEAAGELVIRAHRGWRNSDLAQGMRIKLGQGLSGQAVVTGEVVVTGDVRGD
                     PRLAVPRFAEEGVQAMALAPMRARGRVVGVFSVMHYTPYEFTEDAIRFLTALADQIGV
                     AIDNAQLYEAEYIRRRTSEALRHISGALASTLDLDQAFRITLQHLPEVVHYDRAGIAL
                     LHGDQFRMCAAHGFDSIDMVLRTQAPLQPDSLLGRLIREKRPLIVSDGQDQSVWGTGA
                     LDDPGLRSRMGAPLLARLDTVGALIVESRRARAYLEEDAAILFTLANHLAIAVEKARL
                     FTAESRRAAQLAVINEVARHITATLDLDELLQRAASLIHQHFGCRSVGLFALDPHARE
                     SVFSHGAGEWGERIERGHRQSIDRGLLGRALRTGQLVVANDLAEAAEYFSPIGEKASG
                     SALVIPLQRSGAVIGALAMRHADVDHFRGDVLETAQTLADQLSIAVDNARLYQQTKRR
                     LDELTVVHEMALAGTSALDVGEVSQRAVEALQRRLNFDFMALFLLDAPGAALELRATS
                     KPEEASTRHARIEVGRGLVGTAAQTGQLVNSEDVLRDARYLPGLPGIRSELAVPLTVG
                     ERVVGVLDAQSARPSAFSHDDERLLLTVASQLAVMLDKARLHQETEKRLKDVLTLQSF
                     AQQISGSLDLSEVLDSIVITLKEVLSCRGVSIALLVPDSQMLEIRAAAGIQARWKREA
                     KLKLGEGISGKVASTARSIYVPDTLAEENFIFFDPVVRSLLCVPLTAKDRVIGTLTVD
                     QSVADAFTDGDERLLTIAAAQAAVAIENAQLYEELKERARKLEHAYRELQEADRLKDE
                     LVQNVSHELRTPLTFVKGYVELLLEEDMGPINERQRESLAIVAEKASQVTRLVSDIIF
                     LEQIERESLQLGDLQVVEVAQLALQGCEVTAAAAGIRLRLEVEPDLPTISADRDRLNQ
                     VFDNLLANAIKFSPSGGVITVSVYREMDAILVTVADMGIGIPRDRLHRVFERFYQVDG
                     SSTRRFGGAGVGLAIVKRIVEAHGGRIWVESEPGKGTTFYFTLPFSRPPARTGSSP"
     gene            complement(11265..11573)
                     /locus_tag="AC162G_00690"
     CDS             complement(11265..11573)
                     /locus_tag="AC162G_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074363.1"
                     /translation="MILLNIPVSVSGLAAVMVVLYILAALSRRLGAVTKMKPYYRGFY
                     VAMACLAVAIGAAVLRQPMMPSDLSIYLFLFYLPLLLSGIISLVVAYRYWGWLFRERT
                     "
     gene            complement(11570..11860)
                     /locus_tag="AC162G_00695"
     CDS             complement(11570..11860)
                     /locus_tag="AC162G_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074364.1"
                     /translation="MIPLAGILTIAFWSIVEILLVILYAIARFYQATSGQVSHYRLFV
                     IPMILLGAGGVRYALLGDFVDDPLGDALFVAGSVLLVTLSAWLMRLMTGGRR"
     gene            complement(11981..12745)
                     /locus_tag="AC162G_00700"
     CDS             complement(11981..12745)
                     /locus_tag="AC162G_00700"
                     /inference="COORDINATES: protein motif:HMM:NF018455.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAD55 family ATPase"
                     /protein_id="HIR5074365.1"
                     /translation="MSERVKTGIAGLDEMLGGGFLSETANLVEGAPGTGKSTLGMQFI
                     VNGIQRYDEPGIILTFEEFPAQYYRDAETFGWPFRALETAGKLRVIMTSPEVSRADLE
                     RVGGRIESHVREIGARRILVDSLSHFERITRDPIELRGVVYSFVNALKREGLTSLLTR
                     ESPTLIGEGDLSDEDVAFIMDSFLMLRYVEIDSAVRKALLVLKVRGSEHAKDIRQFEI
                     TGNGIEVRSKFEGREGILSGSPHRMAESFVEAFVRR"
     gene            complement(12742..13119)
                     /locus_tag="AC162G_00705"
     CDS             complement(12742..13119)
                     /locus_tag="AC162G_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074366.1"
                     /translation="MTNAGGDLAARLMGDMDPELLDFLKTKVNSFVKWDLVRFFYENP
                     NSTDTADNVARYVGRDVSAIEVELGELVGTGVLERTRISDLWVYSLASDAETRKLVDQ
                     FILACDDRQFRVKAIYHIIRGMR"
     gene            complement(13406..16342)
                     /gene="secA"
                     /locus_tag="AC162G_00710"
     CDS             complement(13406..16342)
                     /gene="secA"
                     /locus_tag="AC162G_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR5074367.1"
                     /translation="MLKNLFTKVVGDPNDRELARLHPLVDQINALEPELERLSDGDLR
                     AITDEFRARVAEATGELQAQLDTARAEAQAEEDLDARRRLDLNVKKLDDELFKIEQGL
                     LDGFLPRAFAAVREAAKRTITQRHFDVQLIGGIVLNQNRIAEMKTGEGKTLVATLPLY
                     LNALAGHGAHLVTPNDYLSKLGAQWMGPIYHALGLTVGVIQHESAFRFDPDFKTEDDR
                     YDKLRPVPRREAYTADVTYGTNNEFGFDYLRDNMVWSLSQTVQRPLYYAIVDEVDNSL
                     VDEARTPLIISGPAEESSDNYRKFAELVRRLRPSTNGDNKDEEPDGDYVVEEKTRNAT
                     LAESGVEKVERALSLDNLYSPENSALLPYLDNALRAHAVYKRDKDYIVKDGEVIIVDE
                     FTGRLMYGRRYSEGLHQAIEAKENVTVQRESLTYATITFQNYFRMYRKLAGMTGTAKT
                     EEEEFQKIYNLDVVVIPTHRPMIRDDYADVIYKTEAAKFKAVVDEIDELHEMGRPVLV
                     GTVAIETSERLSGTLRRRGIPHNVLNAKEHEKEAGVIAQAGRAGTVTVATNMAGRGVD
                     ILLGGNPEGLARETLRRQGKDLTQITKEERRAALEEAERKCAEEKKKVIELGGLHILG
                     TERHEARRIDNQLRGRAGRQGDPGSSRFYISLEDDLMRRFGGDRIKGIMDWAKMEEDV
                     PLEHGLLSKTIEQAQTRVEGYNFDVRKHVLEYDDVINKQREVIYAHRRKILTEKNLRP
                     IIFDMIADEIGAMTRQHTQAKYADEWDLGGLMSAARGLVPLPATLTIQDWKGLGADEI
                     EDQLLEYAEKAYDEKARQLGEIMPEAERITLLRAVDLHWVRHLTDLDILREGIGLRAY
                     GQQNPLIAYKKEAFETFDEMNESIRSQVARDIFRVQASSPSARPDQAAAPAARATRAN
                     LTGGAKGQRPVRKVAVETVGRNAPCPCGSGKKYKHCHGRPGAEPLPAGVGAKARPGAV
                     QGRPAK"
     gene            16627..16791
                     /locus_tag="AC162G_00715"
     CDS             16627..16791
                     /locus_tag="AC162G_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074368.1"
                     /translation="MEKTFSHLFGVITDLGPKGAGSTLATRIDIPVADHLMLIDEIAS
                     VLHIDFPPVL"
BASE COUNT         2964 a         5364 c         5437 g         3055 t
ORIGIN      
        1 ccggcctcga aacggtataa tagggctgtg gggagataga accttggtgt cttcgtgtct
       61 tggtggtgtg cgaatcccgg gagtgcgcgg attgagacag cggaggcagg aaagatgacc
      121 cgcgttcggc gcgcgctgct gtacatgccg ggcgacgaca tgcgcaagat cggcaaggcg
      181 gcgacgctag acgtcgactg cgtgtgcttg gacctcgaag acggcacggc gctcaaccgc
      241 aagcaggcgg cgcgcgagac gatcgtccag gcgctgcgca cgctcgactt cggccgatcc
      301 gagcggttgg tgcgcatcaa cccggttggg tcgggcatgg aggtcgacga cctggcggcg
      361 acgatcgacg gccggcccga tggctacgtg ctgccgaagg tcgagtccgc cgaggaggtc
      421 gagtggctgt gctggcggct gaccggcgcc gagcaggcgc gcggctggcc gctcggctcg
      481 accaacgtgc tggccatcgt cgagacagca cgaggcatca tcaacctacg ggacatctcc
      541 ggctgcgacc ggcgcctggt cgcgctgatc ttcggcgcgg aagacctggc cggcgatctc
      601 ggcgcgacgc gcacgcgcga aggatgggaa gtcttctacg cgcgcagtgc ggtggtgacg
      661 cacgcggccg cgttcgatct ccaggttatc gacatggtgt gcatcgactt ccgggacaca
      721 gaagccctga tacgcgagtc gctcgaaggg gcgcgcatgg gcttcaccgg caagcagatc
      781 atccatccca atcaggtcga gccggtgcag gcggcgttca cgccgtcgga cgaggcgatt
      841 gcacatgcca ggcgcatcgt cgaagcgttc gaggcgcacc aggccgaggg tgcgggcgcg
      901 ttcgcgctgg acgggaagat ggtcgatatg ccggtcgtca aggcggcgca gaaggtgctg
      961 gaaaaagcaa aagcagcagg aaaggtgtaa cgattaaaat tctcatcgcc gccgacatgg
     1021 aaggcatttc cggcgtggtg cactgggacc acgtcacgcc cgggcacgcc gagtacgcgc
     1081 gcttccggcg tatcatgact cacgacgtca acgccgcgat cgccggcgcg ttcgacgccg
     1141 gcgccgagga ggtggccgtc accgatggcc accgggacaa cttgaatata ctggtcgagg
     1201 agcttgatcc acgcgcgcgg ctgaattccg gcaggccgtc gccgctgtcc atggtgcaag
     1261 atatcggcat ggacgtaagc ggtgtgatgt tcgtgggcta tcacgcgcgc gccggcgcgc
     1321 aaaacgccat cctcgatcac acctggtcgg acatgtgcgt ggcgaacctg tggctcaacg
     1381 gcaggctcac cggcgagatc ggcctgaacg cggcggtgtg tggccacttc gacgcgccgg
     1441 tgatcatgat ttccggcgat cagacggcgt gcgccgaggc agtcgagacg ttgggcgccg
     1501 tcgaggtcgc cgtcgtcaag cacgcgaccg ggcggatggc agccgaatgt ctgccgcccg
     1561 aggccgcgca gaggaagatc cgcgaggcgg cgggccgcgc cgtcaaccgg ctgcgcatgg
     1621 gtcgggccgc gtcgccctat cgcccggagt cgccgatcac cgtcgcgatc gagttcacga
     1681 agtccgagat ggccgatcgg gcaatgcttt tgtccggcgc gcgtcgggaa ggcaggcgcg
     1741 tggaactgac gacggaagat atgccgtcag cgtaccggct tttccgcgcg gcggtgacct
     1801 tagcgaaagg atgacgacga cgaatgacca aagaccagag acgaagacct gccggaatgc
     1861 aggcgcgcgc ggacacgccc gctttagagt tctaccccct gacgccggat cgttggcgcg
     1921 atcttgaagc gttattcggc ccgcgcggcg cggtcggggg atgctggtgc atgtggtggc
     1981 gcataaagcg gacggaattc gaacggcgcc agggcgacgg caataagcgc gccttcaaga
     2041 agatcgtcaa atcgggcgag gagccgggca tcctcgccta cgccggcggc caaccgatcg
     2101 gctggtgcgc cgtcgcgccg cgcgaggcgt atcccgttct cgatcggtcg ccgacgctga
     2161 agcgcgtcga cgaccggccc gtgtggtcgg ttacgtgctt cttcatcgcg cgaccctggc
     2221 ggcgccaggg tgtgacggtc gcgctgctca aggcggctat cgagtacgca gcccggcgcg
     2281 gcgcgacgat cgtcgagggc tatccggtcg cgccgaagaa agacagcatg cctgagatgt
     2341 acgccttcac cggcacggcc tccgcgtttc gcaaggcagg cttcgtcgaa gtggcgcgcc
     2401 gctcggagac gcgcccgatc atgcgataca tgctcccaat cccttgagtt tcatcagttc
     2461 ccggcaaagg ggtcctgcat aaggaactaa aggaactcaa ggaaagggag gttcatggga
     2521 acttctgaac tgaatgttgt gaccggcgca ttcggctata ccgggaagta cgtggcacgc
     2581 cgcttgctat ccatgggcaa gcgcgtcaag acgctgacca cgcatcccga ccggcccagc
     2641 ccgttcggcg atcgggtgag cgtcgccccg ttcgacttcg accggcccga ggccttgatc
     2701 gagagcctgc gcggcgcggc ggcgctgtac aacacctact gggtgcgctt cgactacggc
     2761 tcggcgacct tcgaggcggc ggtcgaaaac accaagacgc tgttccgcgc ggcgaaggag
     2821 gctggcgtcc ggcgcgtcgt gcacgtcagc gtctccaacc cgtccgagga ttcgccgctg
     2881 tcctatttca gggggaaggc gctgcttgaa aaggcgttgg tcgaatccgg cctgtcgcac
     2941 gccatcgtcc gcccgacggt gaccttcggc gacgaagaca ttctgatcaa taacatcgcc
     3001 tggctgctgc gccgtttccc ggtctttgcc attctgggtg agggtgatta ccgggtacag
     3061 cccatcttcg tggaagacgt ggccgaactg atggtaagcg ccggcagcca gagcggcaat
     3121 ctggtcgtcg atgcggccgg cccggaggtg tacacgttcg acacgctggt gcgcaccatc
     3181 aagagcgctg tgcgcagccg ggcgcggatc gtccacctgc cgccgcggct tgcgctgttc
     3241 ctgtcgcaag tgatcggcgt cttcgtcggc gacgtcgtgc ttacgcagga agaggtcgac
     3301 ggcctgatgg ccggcctgct ggtgtcaagc ggcccgccga ccggtcgcac gcacctgagc
     3361 gaatggctgc gcgagcatgc ccatacggtc ggggcgaaat acgcgtcgga agtgacgagg
     3421 cattttcgat cgtagggact ggaggttaga gattagagat tggagattgg agattggagg
     3481 ctggagattg taggttggac tgcctgcttg agatattctc ataatgggtc aggctttgcc
     3541 ggcaggcggg ggtgtggtaa actcgccccg aactgatctg catcgtggag accgcgcatg
     3601 gctaagaaag gccgtgtctt ttccggcgcc cgcccgacgg gccgccagca cctcggcaat
     3661 tacctcggcg cgattcaaaa ctacgttgcg ctgcaggctg actacgagtg tgtttattgc
     3721 atcgtcgacc tgcacgcgct gacgacgctg gagcagaccg acgatctcaa agcctatacg
     3781 cgcgagatgg tgctcgactg gctcgccgcc ggcatcgacc cggccctggg caccatcgtc
     3841 ttcgtccagt cgcacgtgcc catggtcacc gagctgcact ggatcctatc gactatcacg
     3901 tcgctcggca agctgaccga tctgccaacc ttcaaagaga aagtgcgcca gcagccgcac
     3961 aacgtcaact acgcgctgcc cggttatgcg gtgttgatgg ccgccgacat cgcgctgtat
     4021 aagaccgacg tcgttccggt cggcgtcgac caggaaagcc atctggaatt cacgcgcgag
     4081 atcgtccgca ccttcaacca ccgcttcaag ccggtgctcg tcgagccgca gatgaagctc
     4141 accgagtatc ccaagatcct cggcatcgac ggcgagaaca agatgagcaa atcgctcgac
     4201 aatcacatcg agctggcggc gacgccggaa gagacgcagc agcgcgtgat gatgatggtc
     4261 accgacccgc agcgcacgca ccgcaacatt cccggccggc cggaagtgtg caatgtttac
     4321 tcgctgcacc gggtcttctc gccgggccag gtcgatcaga tttacacaga ttgcacgacg
     4381 gcgggcatcg gctgtgtcga ctgcaagaag attctggcgc gcaacctgaa cgcacacctc
     4441 gaaccgttcc gcgcccggcg cgccgtgctt gccgcgcagc ccaacctggt cgacgacgtg
     4501 ctccaggatg gcgcgcggaa ggcgacggcc attgccgcgc aggtgttggc cgaggtaaag
     4561 gacgccgtcg ggctgccctg atgcctgaac tccccgaagt cgaatcctac gtgcgcgccc
     4621 tgcgcgcgcc cctgatcggg cgtaccatta ctgccgtgca cgtgtactgg ccgcgcaccg
     4681 tcgcgcaccc atcgcccaga gagttcacgc ggcgcctgcc cggcctgcgc gtgaacgcga
     4741 tcgaccgccg cgccaagtac ctggtcttcg cgctgagcgc caaatcgtct caagtgccta
     4801 ccgattacct actcatccac ctgaagatgt ccggccaact cgacgtgaca tcgcacccaa
     4861 agcctattga caagcacgat cgcgtgatct tcgatctatc gggcggccgc cggctgtgct
     4921 tcaacgacat gcgcaagttc gggcggctgt acctggtgcc gcatccagag gttgtcaccg
     4981 ggcggctcgg ccctgagccg ctgagcgatg aattcacact agacatcttc aaacggcgac
     5041 tcgggaagcg gaaaggccgc ctgaagtcgc tgctgctcga tcagacgttc gtcgccggga
     5101 tcggcaatat ctacgccgac gaagcgttgt ggctggccag gctgcatccc ttgcggcgcg
     5161 ccgattcgct gagcgacgcc gagacccggg cgttgtatcg ggctatccgc gctgcattgc
     5221 gggagggcat caagcggaac ggcgcgtcgt tcgatcgggt ataccgatcg ggcgactacg
     5281 agtttcgcgt gtacggtcgc gccgatcggc cctgccgccg ctgcaggcag ccgatccgcc
     5341 gcaccgtcgt cggacagcgg ggtacttact tttgcgcgac ctgccaatca ctcagaggtt
     5401 gagactggaa cgatggagcc ggagcaactc attctcatcg tgggagcgct gctgatcggt
     5461 gcgctcctgg tgctgggagg cattttggcc ggactgcgcg gccgccgccg gagcaaatcc
     5521 gatcgtgaca gtctgccggc cgctgcctcc gagcaggtgt cgaagcgaga gccgccttcc
     5581 tggcggaacg aatggcagcg gaaactgccg ccagatgtgg tgttgctggc gcgcgacgcg
     5641 gccaccggcg agtgggtcgt cgagatcgaa ggccagcggt atcgccggct cagcgatgtc
     5701 cacgacgaca aagcggcgac gaagatcgtc agcgccattg atggactcaa agccttcgcc
     5761 gggcttgcct cgaaggcatc gcccgaatcg acagcgcctc agcgtagcag cgtcgtcagc
     5821 gatcccggat cgctcgtgcc gcctccgacc gggccgcgca gaccgaaact ggctacctac
     5881 tctgccccgg agggcagcat catcgctcaa atcgagcaga tccttcagtt ggaattggga
     5941 cggcgccccg acctggcgaa ccgctcgatt cacatgggcg cgttgcccga tggcagcctc
     6001 gtggtcgagg tcgacggcag tttctatcga actccagacg agataccgga tgcggccgcg
     6061 cgcgaaatcg tgctgcgcgc cgtgcgcatg tgggagacat cgagctaaac tcgataccgt
     6121 tcaaacaaag accggtgtca ccgcagactg cgtagcgagc cgaggatgcg aagtggattc
     6181 acgcgcccta cgtaagacct tccagcagcc ggtcgaattc ggccatcact ttctgttcca
     6241 tctcgcggcg cacagcctcg ggcagaaaag ccgctcgcag cgcgttcacg ttcatccggc
     6301 gcaagtggct caggcgaatg cccatcgtca tcagcgccac cagatattcg tccgtgagcg
     6361 tcgtatccga cacgctggga tcgtcggtgt tgagcgtcac tgccacgccc agcatgtaca
     6421 ggtcgcgcag cgggtgatgg ctgaaattgt gcatgacgcc tgtctgcaag ttgctggtag
     6481 ggcacacttc cagggtgacg tgcctttcgc gcagcagctt gatgacgtcc gagttctcga
     6541 tcgcccgaac gccgtgcccg atgcgcgtgg cgcccatatc caggacggca tgctgcacgt
     6601 tgtccgcgcc gcccgcttcg ccagcatgga tcgtgatgcc caggccggct tcgcgtgcct
     6661 cacgaaagac cggcgcgaag ggcgccgcgt cgaagttgac ctcatcgccg gcgagatcca
     6721 gcccgaccat accgcgctcc ttacgggcga tggcgacctc cgcgatccgc ttggcgtttt
     6781 ccactccctc gttccgcacg gccgtcgcaa tcagcctcac tgtgatgcga tagtccgctt
     6841 gcgcccgatc caccgcttcg atcacccagt cggtcacctc ctcgtatgaa aaaccctgcg
     6901 tgcgggccag cgccgcggga ttgaagcgca gctcgagata atgcacatgg tcctgggccg
     6961 cgtcggccac cgcttcgtag gcgacccggg cgaccgcttc aggcgtcttg tagaagcggc
     7021 gaagcaggtg aaacttttcc aggaagcggt gaaaattggc gggatcgtcg gtgatctgga
     7081 cgtagggccg caagtcttcg atgttataag atggcaggtc cacgccgtgc tcttgggcaa
     7141 tctcgaccag cgtcgagagc cgcagcgatc cttccaggtg gcggtgcaga tcggccttgg
     7201 gcaggctgtg cacgacgtgg cgcagagcct gccctgcgac ccccgaaggg gggagtgccg
     7261 aagggtccga caagcgcagc ggggcggcgt cggagcgtcg gacctgattc atggggagct
     7321 gcccgtccgc gccggcgggc ggctgaaagg aagtgtgaaa tagaacgtcg tgcctttgcc
     7381 cggctcgctc tcgacccaga tacgcccacc gtgcgcttcg acgatgcgct tgacaatggc
     7441 caggccgacg ccggccccgc cgaatcgccg cgtggacgag ccgtcgacct ggtagaaacg
     7501 ctcgaacact cgatgcaacc gatcgcgcgg aatgccgata cccatgtccg cgacggtgac
     7561 caggatcgcg tccatctccc gatacacact gacggtgatg acgccgccgc tcgggctgaa
     7621 tttgattgcg ttagccagca ggttgtcaaa cacctggttg agacgatcgc ggtccgcgct
     7681 gatggtcggc aggtccggct ccacctccag gcgcaggcgg ataccggcgg ctgcggccgt
     7741 gacctcgcag ccctgcagcg ccaactgcgc cacctcgacc acttgcaggt cgccgagctg
     7801 tagcgactcg cgctcgatct gctccagaaa aatgatgtcg ctgaccaggc gtgtgacctg
     7861 gctggctttt tcggcgacga tagccaggct ctcgcgttgg cgctcgttga tcggccccat
     7921 atcttcttcg agcagcagtt cgacatagcc tttgacgaaa gtgagcggtg tgcgcagctc
     7981 gtgactcacg ttttgcacta gctcgtcctt gagccgatcc gcctcctgaa gctcgcggta
     8041 cgcgtgttcc agcttgcggg cgcgctcttt cagctcctcg tacaactgcg cgttctcgat
     8101 ggcgacggcg gcttgcgcgg cggcgatcgt caacaggcgc tcgtcgccat cggtgaaggc
     8161 atcggccaca ctctgatcga cagtcagcgt cccgatgaca cgatccttag cggtcagcgg
     8221 cacgcacagc agcgagcgca cgaccggatc gaagaagatg aagttctctt cggcaagcgt
     8281 gtcgggcaca taaatcgacc tggcggtgct ggcgaccttg ccgctgatgc cttcgccaag
     8341 cttgagctta gcttcgcgct tccacctagc ctgaatgcct gccgcggccc ggatttccag
     8401 catctgcgaa tcggggacga gcagcgcgat gctcacgccg cggcagctca gcacctcttt
     8461 gagggtgatg acgatcgagt cgagcacttc gctcaagtcg agagagccgc tgatctgctg
     8521 ggcgaaggat tgcagcgtca gcacgtcctt caggcgcttc tccgtctcct gatgcaggcg
     8581 ggccttgtcg agcatcaccg ccaattggct ggcgacggtg aggagcaggc gctcgtcgtc
     8641 gtgcgagaaa gcagatgggc gcgcgctctg cgcgtcgagc acgccgacga cgcgctcgcc
     8701 caccgtgagc ggaacggcca actccgaccg gatgccgggc aggcccggta gatagcgcgc
     8761 atcccggagc acatcctctg agttcacgag ctgtcccgtc tgcgccgccg tgccgaccag
     8821 gccgcgcccg acctcgatgc gtgcgtggcg cgtgctcgcc tcctccggct tcgaagtcgc
     8881 gcgcagctcg agcgccgcgc ccggcgcgtc gagaagaaac agcgccatga agtcgaagtt
     8941 cagtctgcgc tgcagcgcct cgaccgcgcg ctggctgacc tcgccgacgt ccagcgccga
     9001 cgtgccggca agtgccatct cgtgcaccac ggtcaattcg tccagacgcc gcttggtttg
     9061 ctggtacaac cgcgcgttat cgacggcgat ggacagttgg tcggccagtg tttgcgccgt
     9121 ctcgagcacg tcgccgcgaa agtgatccac gtctgcgtgt cgcatggcga gcgcgccgat
     9181 gacggcgccg ctacgctgaa gcgggatgac gagcgccgat ccgcttgcct tctcgccgat
     9241 cggcgagaag tattccgcgg cctcggccag atcgttggcc acgacgagtt ggcccgtgcg
     9301 cagcgcacgc ccaagcaaac cgcgatcgat cgactggcga tgaccccgtt cgatgcgctc
     9361 gccccactcg cccgcgccgt ggctgaagac tgattcgcgg gcatgggggt cgagggcaaa
     9421 caggccgacc gagcggcagc caaaatgctg atgaatcaga ctggcagcac gctggagcag
     9481 ctcgtccagg tcgagcgtcg ccgtaatgtg tcgagcgacc tcgttgatca cggctaactg
     9541 cgcagcgcgg cgagattccg ccgtgaagag gcgcgctttt tcgacggcga tggccaggtg
     9601 attcgccagc gtgaacagga tagccgcgtc ttcttccagg taggctcgcg cgcggcgcga
     9661 ctccacgatc aacgctccga cggtgtccag gcgcgcgagc agcggagcgc ccatccgcga
     9721 tcgaaggccg ggatcgtcca acgcacccgt gccccaaacg gactgatcct gcccgtcgga
     9781 gacgatgagc ggacgcttct ctcggatcag gcgaccgagc agggaatcgg gctgaagggg
     9841 ggcctgcgtg cggagcacca tgtcgattga gtcgaaaccg tgcgcagcgc acatgcggaa
     9901 ctggtcgccg tgaaggagcg cgatgccggc gcggtcgtaa tggacgactt cgggcaagtg
     9961 ctgcagggtg atgcgaaacg cttgatccag gtccagcgtt gaggcgagcg cgccggagat
    10021 gtggcgcagg gcctcggacg tgcgccggcg tatgtactcc gcctcgtaaa gctgcgcgtt
    10081 gtctatggcg acgccgatct gatcggccag cgccgtcagg aatcggatgg catcctcggt
    10141 gaattcgtac ggcgtatagt gcatgacgct gaacacgccg acgacgcgtc cccgcgcacg
    10201 catcggagca agcgccatcg cctgcacgcc ttcttctgcg aagcggggca cggccaggcg
    10261 tggatcgccg cgcacgtccc cggtgacgac aacctcacct gtgacgacgg cttgcccgga
    10321 cagcccctgg ccgagcttga tgcgcatgcc ctgagccaaa tcgctgtttc gccaacctcg
    10381 gtgggcacga atcaccagct cgccggccgc ctcttcgatc aggctgatcg cgcccgcctc
    10441 gacgcccatg acgtcgagcg ctttatcgag cgcgatgcgc agcgtctgct ccaggtcgag
    10501 ctcctgactg accgtcgcgg cgactttgtt cagcgcggcc atgtgatcgg cgcggcgccg
    10561 ggcttcctcc agcagccggg cattctcaat cgcgatagca gcctgcgtgg cgaatacttc
    10621 ggccacgcgc acgtcatctt cgccgtaagc tgcccgctcg acgtgctcca gcgcgagtac
    10681 gccgatcaac tgatcgcgca cgagcagcgg cacgcccagc cagcagccaa cgcttccagg
    10741 cccctcgact ccccctgcgc tccgctcagg gcttcggctc agggcagacc tttccagacc
    10801 cggccagcgt ggatcgcccc gcgtatcgct cagcaagatc gagcgctgcg tcgtgacgac
    10861 ttcgcgccac ggactgaggt cggtgtagtc gcgatcacgc gtagcgtgga cgagcggcgc
    10921 gccgtggggg ctgcgcgtca ctactgagcg cagaactccg tcttccgtca aaaagatcga
    10981 ggcgctgtgg taaggcagca cgcgctggag ctgcgtgagg atcagttccg gcacgtcggc
    11041 catgcgcgcc gcagagttga gcgcgagcgc aatctcctgg agcacggtca acgaccggcg
    11101 atcggcgccg gatggcctgc gcgccgcacc gccttccctc gtcgtggagc ggagggggtc
    11161 gcccgcgtcg acggcgtgcg agactgcctc cccttcgact ccgctcaggg caggattcag
    11221 cggaagctga tttttgcgat cggggctttc gggtgcatcg ctcactaggt acgctcccgg
    11281 aacagccacc cccagtatcg ataagccacg acgaggctga tgatgcccga cagcagcagc
    11341 ggcaggtaga agaggaagag ataaatggaa agatcggacg gcatcatggg ctgccgcagc
    11401 actgccgcgc cgatggcgac ggcgaggcac gccatggcga cgtagaagcc gcgatagtac
    11461 ggcttcattt tcgtgacggc gccaagccgg cggctcaacg cggccaggat gtacagcacg
    11521 accatgacgg cggccaggcc ggaaacactg acggggatat tgagcaggat caccgccgcc
    11581 ctccggtcat caggcgcatc agccaggccg atagcgtgac caaaagaacg cttcccgcta
    11641 cgaacagcgc atctccgagc ggatcgtcca caaagtcgcc aagcagcgca taccgcacgc
    11701 cgccggctcc cagcaggatc atcggaatga cgaacaacct gtagtgcgat acctgccccg
    11761 aagtggcttg atagaagcga gcgatcgcgt acaggatgac gagcaggatc tcaacaatgc
    11821 tccaaaaggc gatggtgaga atgccggcca gcgggatcat agcggactgg gaaccctgcc
    11881 ctgagcggag tcgaagggcg tgcctcgtcg tgaggagcac aggtcacaga gcctgccctg
    11941 cgcgaaggcg aagggacagc ggcggcgggg caggccgccg tcagcgccgc acaaaggcct
    12001 cgacaaacga ttcggccatg cggtgcgggc tgccgctcag gatgccttcg cggccctcga
    12061 acttggagcg cacctcgatg ccgttgccgg tgatctcgaa ctggcgaatg tctttggcat
    12121 gctcgctgcc gcgcactttc aggacgagca gcgctttgcg gacggcgctg tcgatctcga
    12181 cgtagcgcag catgaggaag gaatccatga tgaaggccac gtcctcgtcg ctcagatcgc
    12241 cttcgccgat caaggtcggg ctttcgcgag tcagcaggct ggtcaatccc tcgcgcttga
    12301 gcgcgttaac gaaggaatac acgacgccgc gcagctcgat cgggtcgcgc gtgatgcgct
    12361 cgaagtgcga caggctatcg accaggatgc gccgggcgcc gatctcgcgc acgtgcgatt
    12421 cgatgcgtcc gccgacgcgt tccaggtcgg cccggctcac ttcggggctg gtcatgatca
    12481 cgcgcagctt gccggccgtc tccagtgcgc ggaagggcca gccaaaggtt tccgcatcgc
    12541 gataatattg tgccggaaac tcttcgaagg tcaggatgat gcccggctcg tcgtagcgct
    12601 gaatgccgtt gacgatgaac tgcatgccca gcgtgctctt gccggtgccg ggcgcgcctt
    12661 cgaccagatt ggccgtctcg ctcagaaaac cgcccccgag catctcgtcc agtccggcga
    12721 ttcccgtctt gactcgctcg ctcatcgcat gcctcggata atgtgataga tagccttcac
    12781 gcggaattgc cggtcgtcgc aggccaggat gaattgatcg accagtttgc gcgtctcggc
    12841 gtcgctggcc agcgagtaca cccacagatc gctgatccgc gtgcgctcca gcacgccggt
    12901 acccaccagc tcgcccagtt cgacctcgat ggcgctcacg tcacgcccga cgtagcgcgc
    12961 cacattgtcc gccgtgtctg tcgaattggg gttctcataa aagaagcgca cgagatccca
    13021 tttgacaaag gagttcactt tggttttcaa gaaatccagc agctcgggat ccatgtcgcc
    13081 catcagccgg gcagccagat cacctcccgc attcgtcatc gatgacctcc tcaacgctac
    13141 tctccgtaaa gaactggact tgggcaaccc cgctgggcgg ggcgcaagca gctcgccgga
    13201 gtcaaggctt tagccggaca tatctggcag tcaacaccgg ctgaatcctc gattccgacc
    13261 caagatgtgg cacttgccaa actccagtaa ggaactgccg ggcgctaccg gcggattgtc
    13321 caacgtttcg aatcgcgccg cccgcgcacc gcaaacacac gtggccgagc ctcctgaacg
    13381 cggccgcctc tgggaatagg tccggttact tcgccggcct gccttgaacc gcgcccggac
    13441 gcgctttcgc gccgacgcct gccggcagcg gctcggcgcc gggccggccg tggcagtgct
    13501 tgtatttctt accgcttccg cagggacacg gcgcgttgcg cccgaccgtc tccacggcaa
    13561 ccttgcgcac gggtctctgg cctttcgcgc cgcctgtgag gttggcgcgc gtggctcgcg
    13621 cagccggcgc cgcggcctga tctggccgag ctgaaggcga agatgcctgc acgcggaaaa
    13681 tgtcgcgcgc cacctggctg cgaatcgact cattcatctc gtcgaaggtt tcgaaggctt
    13741 ccttcttgta tgcgatcagc gggttctgct ggccgtaggc gcgcaggccg atgccttcgc
    13801 ggaggatgtc gagatccgtc aggtggcgca cccagtgcag atcgacggcg cgcagcagag
    13861 tgatccgctc ggcttccggc atgatctcgc cgagctggcg cgccttttcg tcgtacgctt
    13921 tctcggcata ttcgagcaac tgatcctcga tctcgtccgc gccaaggccc ttccagtcct
    13981 ggatcgtgag cgtggccggc aacgggacta ggccgcgcgc ggccgacatc agcccgccca
    14041 ggtcccactc gtcggcgtat ttggcctgcg tgtgctgccg tgtcatcgcg ccgatctcgt
    14101 cggcgatcat atcgaagatg atcggacgga ggttcttctc cgtcaggatc ttgcggcgat
    14161 gcgcgtagat cacctcgcgc tgcttgttaa tgacgtcgtc gtactccagc acgtgcttgc
    14221 gcacgtcgaa gttgtagccc tccacgcgcg tctgcgcctg ctcgatggtc ttgctcaaca
    14281 ggccgtgctc cagcggcaca tcctcttcca tcttcgccca gtccatgatg cccttgatgc
    14341 gatcgccgcc gaagcgccgc atcaggtcgt cttccagcga gatgtaaaag cgactggagc
    14401 cgggatcgcc ctggcggccg gcgcgcccac gcagctggtt gtcgatgcgg cgcgcctcgt
    14461 gccgctcggt gccgaggatg tgcaggccgc ccagctcgat caccttcttc ttctcttcgg
    14521 cgcacttgcg ctctgcctct tcgagcgcag ctcgccgttc ctctttcgtg atctgggtga
    14581 ggtccttgcc ctgccggcgc agcgtctcgc gggccaggcc ctcgggattg cccccgagca
    14641 ggatgtccac gccgcggccg gccatgttgg tggcgacggt cactgtgccg gcgcgacccg
    14701 cctgggcgat gacgcccgct tccttctcgt gctccttcgc gttcagcacg ttgtgcggga
    14761 tgccccggcg tctgagcgtg cccgacagcc gctccgacgt ttcgatcgcg accgtgccga
    14821 cgagcaccgg ccgccccatc tcgtgcaatt cgtcgatttc gtcgacgacg gctttgaact
    14881 tggccgcctc ggtcttgtag atcacgtccg cgtagtcgtc gcggatcatc ggccggtgag
    14941 tcgggatgac gacgacgtcg aggttgtaga tcttttggaa ctcttcctct tcggtcttgg
    15001 ccgtgccggt catgcctgcc agcttgcggt acatgcggaa gtagttctgg aaggtgatcg
    15061 tcgcgtaagt caggctctcg cgctggacgg tgacgttctc cttggcctcg atcgcctggt
    15121 gcaggccctc gctgtagcgg cggccgtaca tcaggcggcc ggtgaactcg tccacgatga
    15181 tcacttcgcc gtctttgacg atgtaatcct tgtcgcgctt gtacacggcg tgggcgcgca
    15241 gcgcgttgtc gaggtacggc agcagcgcgg agttctccgg cgagtacagg ttgtcgaggc
    15301 tgagcgcgcg ctccaccttc tcgacgcccg actcggccaa cgtggcattg cgcgtcttct
    15361 cctcgacgac gtaatcaccg tccggctctt cgtctttgtt gtcgccgttc gtgctgggcc
    15421 gcaggcggcg caccagctcg gcgaacttgc ggtagttgtc gctggactct tccgccgggc
    15481 cgctgatgat caggggcgtg cgcgcctcgt cgacgaggct gttgtccacc tcgtcgacga
    15541 tcgcgtaata cagcggccgc tgaaccgtct ggctcaggct ccacaccatg ttatcgcgca
    15601 ggtagtcgaa gccaaactcg ttgttggtgc cgtaggtgac gtcggcagtg tacgcttcgc
    15661 gccgtggaac ggggcgcagc ttgtcgtagc ggtcgtcctc ggtcttgaaa tccggatcga
    15721 agcggaaagc gctttcgtgc tggatcacgc cgacggtcag gccgagcgcg tgataaatcg
    15781 gccccatcca ctgcgcgccg agcttggaca ggtagtcgtt gggcgtcacc aggtgcgcgc
    15841 cgtggcccgc cagcgcgttg aggtacagcg gcagcgtggc gaccagcgtc ttgccctcgc
    15901 cggtcttcat ttcggcgatc ctgttctggt taaggacgat gccgccgatc aactgcacgt
    15961 caaagtggcg ctgagtgatc gtgcgcttgg ccgcctcgcg gacagcagcg aacgcgcgcg
    16021 gcaggaaccc atcgagcagc ccttgctcga ttttgaacaa ttcgtcgtcg agtttcttga
    16081 cgttcagatc gagccggcgg cgcgcgtcga ggtcttcctc ggcctgagct tcggcccgcg
    16141 ctgtgtccag ttgcgcctgg agttcgccgg tcgcttcggc gacgcgcgcg cgaaattcgt
    16201 ccgtgatggc gcgcaggtcg ccgtcggaga gccgttccaa ttcaggttcg agcgcgttga
    16261 tctggtcgac aagcggatga aggcgggcaa gctccctatc gttcggatcg cccaccacct
    16321 tcgtgaacag attcttgagc attcagcatc ctttgacggg taattatagc acaatcgaaa
    16381 tcgcttttga caggtactgt ggtcacctga aacacagacc gcacccaccc ccttgcccct
    16441 tcggtttgca ggagagggaa ggggagagtg tccgcgcagc ggacgggaag gggaaggtga
    16501 ggtataaagt aaaaacgccc gccactcaat tgcggggggc agcatgcatg gtttacgcat
    16561 tgttaggagg tttctctggt tggagccagt cttcgagcgt gctattgttt gactgaaggt
    16621 cgcagtatgg agaagacctt cagtcatctg ttcggcgtga tcaccgactt ggggccgaag
    16681 ggagctggtt cgaccttggc gacacgtatc gacatcccgg tcgccgatca tcttatgcta
    16741 atcgacgaaa ttgcgtcagt tttacatatt gacttccccc ccgtactgta atatggtgtg
    16801 tccagtttag catcccagcc
//
LOCUS       DBNHXB010000020         7905 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3024066, whole genome shotgun sequence.
ACCESSION   DBNHXB010000020 DBNHXB010000000
VERSION     DBNHXB010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3024066"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="AC162G_00720"
     CDS             <1..779
                     /locus_tag="AC162G_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HIR5074369.1"
                     /translation="AKEAGIRRIALSLDAPSPEVHDAFRQVPGSWERTMKILRNAQSI
                     GLSAQINTTVTRHNVDLLPDMVQFVQEVGAVQWSVFFLVPTGRGQIADMISPEEHERV
                     FNWLYDLSQRGAFDIKATAAPMYRRVAIERKRLTGVGGEVFFQGAGFQYADGLNRPVK
                     GVNDGRGFLFISHLGEIMPSGFLPIAAGNVREVDVVETYRHHPLFRDLRDPARLKGRC
                     GICEYKVVCGGQRGRAYALTGDYLESDPACIYQPRGRIHA"
     gene            772..1461
                     /locus_tag="AC162G_00725"
     CDS             772..1461
                     /locus_tag="AC162G_00725"
                     /inference="COORDINATES: protein motif:HMM:NF018484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HIR5074370.1"
                     /translation="MPEETPRTLNHFALFTFKDAHGALPPTERAEFHQAWLSGLRVAA
                     KKVDVYQVFPAEDRADILVWSAVPIEDQCHTAAFFERFAQATNPYRAYLQISTTLWGY
                     TRQSQYTKTRSAQEIDPFAEARKPYLVIYPFSKTAEWYLMRREARQGLMNEHIRIGKQ
                     YEDITQLLLYSFGVQDQEFVVVYETDDLMRFSDLVNELRGTEVRRYTLRDTPLHTAIY
                     HPAEETLALWK"
     gene            1452..2501
                     /gene="hemE"
                     /locus_tag="AC162G_00730"
     CDS             1452..2501
                     /gene="hemE"
                     /locus_tag="AC162G_00730"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIR5074371.1"
                     /translation="MEMTDSRFLRACRREPVDRTPVWFMRQAGRYMAEYRAVRAKHSL
                     LEICRQPELAVEVTLQPVRALGVDAAILFADILLPLIPMGIDVEFSAGEGPVIHNPIR
                     APADVSALRTVEPCESLGHVLEAVRLIRRELDGKVPLIGFAGAPFTLASYLIEGGSSR
                     NFVRTKRLMVNDPPMWHALMGKLARVVANYLVAQVEAGAQAVQLFDSWVGALGPDDYR
                     EYVLRHSRLVFDALRATGVPAIHFGTDTATLLPLMKDAGGDVISLDWRTPLDWGWSAL
                     GPEVAVQGNLDPVALFAPRGELERRVRAILKQAGGRPGHIFNLGHGILPETPVENVKA
                     VVEMVHEFSVLEFGI"
     gene            2498..3427
                     /gene="hemH"
                     /locus_tag="AC162G_00735"
     CDS             2498..3427
                     /gene="hemH"
                     /locus_tag="AC162G_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR5074372.1"
                     /translation="MKFEVMIGVLVMAYGGPNSLDEVEPYLLDVRGGRPTSSALIHDV
                     RERYRQIGGRSPILERTQAQAAALEGALNANGQVFKTFVGMRHWHPFVKDALAQMEIA
                     GLRRAVGLVMAPHYSRMSVGAYFKKARDAHSPVEVAPIERWHLLPGYITALADRVRAA
                     LQTFPADVRADVPIIFTAHSLPQRILQWDDPYPDELHATVEAVMQRLGPRPYAFAYQS
                     AAMTPEPWLGPDAGEWIERFAAEGKKHVLIAPIGFVCEHVEILYDVDIAFKRQAEALG
                     MQLERIEMLNDAPQMIAGLAELVRDKAKEAGWL"
     gene            3424..4851
                     /gene="hemG"
                     /locus_tag="AC162G_00740"
     CDS             3424..4851
                     /gene="hemG"
                     /locus_tag="AC162G_00740"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HIR5074373.1"
                     /translation="MTETKRVVIVGGGIAGLAAAYRLRQTAPDVQVVLIESENRLGGK
                     IVTERVDGFVIEGGPDTFLSYKPRGVGLCSELGLEDRLHGTNEKLRRTYVMRRGKLYE
                     LPEGLTGLIPSRFGPMAKTGLISPLGKLRMGLDFFIPPRPGDGDETLASFVERRLGRE
                     LYDRLIEPLLSGIYAGDGEQLSLSATFPQLRQIELEHGCLIKGMLATRRNSLSSNGRK
                     PDSKIRSAFLTPTTGLAEIVEVLEAKLAAVEIRLGARVTHVEANASGYVVALAGGTPL
                     HADAVVLATPAFVTADLVSGLDTQLRNTLKSIPYASTATVSVAYPLSDIPRPLDGYGY
                     IVPRAEGRSILACTWTSTKFPHRAPEGHGLIRAFIGRAGQEDILDRSDDELLTLARDE
                     LRAVLGITAPPMLHRIFRWPKTMPQYTLGHLDRLRGIEQRLAHHPGLFVAGNGYRGIG
                     IPDCIASGEQAAQSALAYIRLLIYA"
     gene            4937..6229
                     /gene="hemL"
                     /locus_tag="AC162G_00745"
     CDS             4937..6229
                     /gene="hemL"
                     /locus_tag="AC162G_00745"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIR5074374.1"
                     /translation="MNIVRSQQLFAEAQTLLPGGVDSPVRAFRAVGGQPLFIDRAEGA
                     YLYDVDGNRYIDYVLSWGPLILGHAHPHVVQAIAQAAARGTSYGAPSPLELELAKLIM
                     QFVPSIEMIRFVNSGTEATMTALRLARAFTGRNKIVKFEGCYHGHADMLLVQAGSGVA
                     TLGLPDSPGVPPATVTDTLVAPYNDIEAVRQLFERFPKDIAAVIVEPVAGNMGVVPPV
                     GGFLAGLREVTSAYGALLIFDEVMTGFRVHPGGAQALYGIKPDLTTLGKVIGGGLPVG
                     AYGGRRDVMQMVAPTGPMYQAGTLAGNPLAMTAGIETLKEIQKPGIWETLEARSAQLA
                     IGLSAAAEDARVRVVLQRVGTMFTAFFTERPVVNWATAKTADTKQYAAYFRAMLERGV
                     YLAPSQFEAGFMSAAHGGEEIGLTVQAAEKALKQLADL"
     gene            6273..6752
                     /locus_tag="AC162G_00750"
     CDS             6273..6752
                     /locus_tag="AC162G_00750"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5074375.1"
                     /translation="MSTMKDGADLEQPGGTPESSQREYPHGPIPSAHAIVLRNDRVLL
                     VKRAHDPGKGRWSLPGGMIRLGETIYEAVQREVREECSMEIEPVRILDVFDSILKDEQ
                     GRIRFHYLVVYLLARYLTGEAQAGSDASEVRWATREELNALDMHPRVRETVHATWLS"
     gene            complement(6777..7514)
                     /locus_tag="AC162G_00755"
     CDS             complement(6777..7514)
                     /locus_tag="AC162G_00755"
                     /inference="COORDINATES: protein motif:HMM:NF025559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HIR5074376.1"
                     /translation="MKPIVPNRRNEQWALLLGVTLGTLGSLALRRRPAVPGRAHRLDL
                     WQRLLAKRLGEVDAALLAARVQARYEELYAHRPRFTHRALRMHLERYILPALALYQIL
                     CETHGDLQAALAEWDDLLGAPEGSEMRKALALLGHLPWKFAIFRAAVRQRMKRDFPPE
                     GWAYEWVEDSDRRVAFNIRSCLYLKVLTAYGVPELTEHFCRLDDLAAEALPLSIAWAR
                     TTTLGRGGPVCDFCWTHTAPNRTGPSA"
     gene            complement(7542..7850)
                     /locus_tag="AC162G_00760"
     CDS             complement(7542..7850)
                     /locus_tag="AC162G_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor-like protein"
                     /protein_id="HIR5074377.1"
                     /translation="MSGMRIGQVTHYYDRLRVAVLALTDVIRVEDYVHIVGHKTDFHQ
                     KVTSLQINHQPVHEAQPGQDVALMVGKRVRPHDVVYRATAEETEQAQKEDILDLLKSE
                     "
BASE COUNT         1475 a         2470 c         2500 g         1460 t
ORIGIN      
        1 aggcgaaaga ggccggcatc cgccgcatcg cgctcagcct cgacgcgccc tcgcccgaag
       61 tccacgacgc gttccggcaa gtgcccggat cgtgggagcg cacgatgaag attctgcgca
      121 acgcgcagtc gatcggcctg agcgcgcaga tcaacacgac cgtcacgcgc cacaacgtcg
      181 atctcctgcc ggacatggtg caattcgtac aggaagtcgg cgcggtgcag tggagtgtgt
      241 tcttcctcgt gccgaccggg cgtgggcaga tcgccgacat gatctcgccc gaagaacacg
      301 agcgcgtgtt caactggctg tacgacctgt cgcagcgcgg cgcattcgat attaaagcca
      361 ctgctgcgcc gatgtatcgc cgcgtcgcca ttgagcgcaa gcgcctcacc ggggtggggg
      421 gtgaggtctt cttccaaggt gcgggcttcc agtatgccga cggcctcaat cgcccggtga
      481 aaggcgtgaa cgacgggcgc ggctttctgt tcatctcgca tctcggcgaa atcatgccgt
      541 ccggcttcct gccgatcgcg gcgggcaacg tccgcgaagt ggatgtggtc gaaacctatc
      601 gccatcatcc gctcttccga gatctgcgcg acccggcgag gctgaagggc cgctgtggaa
      661 tctgcgaata caaagtcgtg tgcgggggcc agcgcgggcg tgcctacgcg ctgaccggcg
      721 actatcttga atctgatccg gcttgtatct accagcccag ggggagaatc catgcctgag
      781 gagacaccgc ggacgctgaa tcatttcgcg ctgttcacat tcaaagatgc tcacggggca
      841 cttccgccga cggagcgcgc cgagttccat caagcatggt tgtcgggcct acgggtcgct
      901 gcgaagaagg tcgacgttta tcaggtgttc ccggcagaag accgcgccga catcctcgtg
      961 tggagcgccg tgccgatcga agaccagtgc cacaccgccg cgttcttcga gcgcttcgcg
     1021 caggcgacga acccgtatcg cgcctacttg cagatcagca cgaccctgtg gggctacacg
     1081 cgccagtcgc agtacaccaa gacgcgctcc gcgcaggaga tcgatccgtt cgcggaagcg
     1141 cggaagccgt acctcgtgat ctatcccttc tcgaagacgg ccgaatggta tctgatgagg
     1201 cgcgaggcgc ggcagggttt gatgaacgag cacattcgca tcggcaagca gtacgaggac
     1261 atcacgcaac tgctgctgta ctcgttcggc gtccaagatc aggaattcgt cgtcgtgtac
     1321 gagaccgatg acctgatgcg cttctcggat ctggtcaacg aactgcgcgg caccgaggtt
     1381 cgacgttaca cgctgcgcga cacgcctctg cacacggcga tctaccatcc tgccgaagag
     1441 acgctcgctc tatggaaatg acggactcgc gttttctgcg cgcctgccgc cgcgagccgg
     1501 tcgatcgcac gccggtctgg ttcatgcggc aggccggccg ctacatggcc gagtatcgcg
     1561 ccgtgcgcgc caaacattcg ctgctggaga tttgccgcca gcccgaactc gcggtcgaag
     1621 tcacgcttca gccggtgcgc gcgctcggcg tcgatgccgc gatcctcttc gccgacatcc
     1681 ttctgccgct gatcccgatg ggcatcgatg tggagttctc ggcaggcgaa gggccggtga
     1741 ttcacaaccc gattcgtgcg ccggccgacg tctccgcttt gcgcaccgtc gagccgtgcg
     1801 aatcgctcgg ccatgtgctg gaagccgtgc gcctgatccg gcgcgaactc gacggcaagg
     1861 ttccgctgat cggcttcgcc ggcgcgccgt tcacgcttgc cagttacctc atcgagggcg
     1921 gctcgtcgcg caactttgtg aggaccaagc ggctgatggt caacgatccg ccgatgtggc
     1981 acgcgctaat gggtaaactg gcgcgcgtcg tcgccaatta tctcgtcgcg caggtggaag
     2041 cgggcgcgca ggcagtgcag ctcttcgaca gttgggtcgg cgcgctcggc cccgatgact
     2101 atcgcgaata cgtgctgcgg cactcgcgcc tggtcttcga cgcgctgaga gcgacgggcg
     2161 tccccgcgat tcacttcggc accgacacgg ctacgctgct gccactcatg aaagatgccg
     2221 ggggcgacgt catcagtctc gattggcgca caccgctcga ctggggctgg agcgctctcg
     2281 gccccgaagt cgccgtgcag ggcaaccttg atcccgtagc gctctttgcg ccgcgcggcg
     2341 aactcgaacg gcgcgtgcgc gcgatcctga agcaggccgg tggtcgcccc ggccacatct
     2401 tcaatctcgg tcacggcatt ctgccggaaa cgccggtcga gaacgtcaaa gccgtagtcg
     2461 agatggtgca cgagttctcc gttttggagt ttggcatttg aaatttgaag ttatgatcgg
     2521 cgtgctcgtg atggcctacg gcggaccgaa tagcctcgac gaagtcgagc cgtacctgct
     2581 cgacgtgcgc ggcggccggc cgacgtcgtc cgctctcatc catgacgtgc gcgaacgcta
     2641 ccggcagatc ggcggacgct cgccgattct cgaacggaca caggcgcagg ctgcagcgct
     2701 cgaaggggct ttgaatgcga acggacaggt cttcaagacc ttcgtcggca tgaggcactg
     2761 gcatcccttc gtcaaggacg cgctggcgca gatggaaata gcggggctga gacgcgctgt
     2821 cggattggtg atggcgccgc attactcgcg tatgagcgtc ggcgcgtatt tcaaaaaggc
     2881 acgcgacgca cattcgcctg tcgaggttgc gcccatcgag cggtggcatt tgcttcccgg
     2941 ctacatcact gccttagccg atcgcgtgcg cgcagcacta cagacgtttc cggcggatgt
     3001 tcgcgccgat gtgccgatta tcttcacagc ccacagcctg ccgcaacgaa tcctccaatg
     3061 ggacgatcca taccctgacg aactacatgc tacggtcgaa gccgtgatgc agcggcttgg
     3121 gcctcgcccg tacgcgtttg cctaccagtc cgctgcgatg acgcccgagc cgtggctggg
     3181 gcctgacgcc ggcgagtgga tcgagcgctt cgctgctgag ggcaagaagc acgtgctgat
     3241 cgcgccgatc ggcttcgtgt gcgagcacgt cgagattctg tacgatgtgg acatcgcctt
     3301 caagcggcag gccgaggcgc tcggcatgca attggagcgc atcgagatgc tgaacgatgc
     3361 gccacagatg atcgcagggt tggccgaatt ggtacgagac aaagccaagg aagcaggctg
     3421 gttatgaccg aaacgaagcg ggtagtgatt gtcggcggcg ggatcgcggg gctggccgcc
     3481 gcctatcggc tccggcaaac tgctccagac gtgcaggttg tcttgatcga gagcgaaaac
     3541 cgtctcggtg gcaagatcgt caccgagcgc gtggacggtt tcgtcatcga aggtgggccg
     3601 gacactttcc tttcgtacaa gccgcgcggc gtcgggctgt gcagcgaact cggcctcgaa
     3661 gatcgactcc acgggacgaa cgagaagctt cgccgcacgt acgtcatgcg gcgcggcaag
     3721 ctctatgaac tgcccgaagg gctgacgggc ttgattccct cgcgcttcgg gccgatggcg
     3781 aagactggcc tgatctcgcc gctgggcaaa ctgcggatgg ggctggactt cttcattcct
     3841 ccgcgacccg gcgacggcga cgagacactc gcctcgttcg tcgagcggcg cttgggccgc
     3901 gaactgtacg atcggctgat cgaaccgctg ttgagcggca tctacgcggg cgacggcgag
     3961 cagttgagcc tcagcgcgac atttccgcag ttgcgccaga tcgaactcga acatggttgt
     4021 ctgatcaaag gcatgctggc gaccaggcgc aatagcctgt catctaatgg ccgcaagcca
     4081 gacagcaaaa tacgatcggc ctttctcact ccgacgaccg gcctcgccga gatcgtcgaa
     4141 gtcctcgaag cgaaactggc cgccgtggag atccggctgg gggcgcgcgt gactcacgtc
     4201 gaagcgaatg cgtccggtta cgtcgtggct ctggccggcg gcacgcccct gcacgccgac
     4261 gcggtcgttc tcgccacgcc cgccttcgtc actgccgatc tcgtctctgg tctcgataca
     4321 caactccgca ataccctgaa aagcattccg tacgcttcaa ctgcgactgt ctccgtcgct
     4381 tatccgctgt ccgatatccc gcgcccgctc gatggctacg gctatatcgt cccacgcgcc
     4441 gaaggccgat cgattctcgc ctgcacctgg acatcgacca aattccctca tcgtgcgccg
     4501 gaagggcatg gtctgatccg cgccttcatc ggccgggccg gacaggagga catactcgat
     4561 cgaagcgacg acgagctgct gacgctggca cgcgacgagc tacgcgccgt gctaggcatc
     4621 accgcgccgc cgatgctgca tcgcatcttc cgctggccga agacaatgcc gcagtacacg
     4681 ctggggcatt tagatcgcct gaggggaatt gaacagcgcc tcgcgcacca ccccggcctg
     4741 ttcgtcgcgg gcaatggcta tcgtggcatc ggcatccccg actgcatcgc ctctggcgag
     4801 caggccgcac aaagtgcact tgcttacatc cggttgctaa tctacgcatg acgccataca
     4861 aaccaccaag gcgccgagac acaaagattg aagaagactt ggcgtcttgg tgtcttgatc
     4921 gtgaacaagg agtcagatga acatcgtacg ctcgcagcaa cttttcgccg aagcgcagac
     4981 gctcctcccc ggcggcgtcg attcgcccgt gcgcgccttc cgcgccgtcg gcggacagcc
     5041 gctgttcatc gaccgagctg agggcgcgta tttgtacgat gtagacggca accgctatat
     5101 cgactacgtg ctgtcgtggg ggccactgat tctgggccat gcccatccgc atgtcgtgca
     5161 agccatcgcg caagccgccg cgcgcggcac gagttacggc gcacccagcc cgctcgaact
     5221 cgaactggca aagttgatca tgcagttcgt gcctagtatc gagatgatcc gcttcgtcaa
     5281 ctccggcacc gaggcgacga tgaccgcgct gcggctagcg cgcgccttca ccgggcggaa
     5341 caagatcgtc aagttcgaag gctgttatca cggccacgcc gatatgctgc tggtgcaggc
     5401 cggatcgggt gtggcgacgc tcggactgcc cgactcgccc ggcgtgccgc cggcaaccgt
     5461 caccgatacg ctcgtcgcgc cgtacaacga catcgaagcc gtgcggcagc tgttcgagcg
     5521 gtttcccaaa gacatcgcgg cggtgatcgt cgagccggtg gcaggcaaca tgggcgtcgt
     5581 gccgccggtg ggaggattcc tggcggggct tcgggaagtc acgtcggctt atggcgcgct
     5641 gctcatcttc gacgaggtga tgaccggctt ccgcgtgcat ccgggtggcg cgcaggcgct
     5701 gtacggcatc aagccggatt tgacgacgct gggcaaagtg atcggcggcg gcttgccggt
     5761 cggcgcgtac ggtggccggc gtgacgtgat gcagatggtc gcgcccaccg ggccaatgta
     5821 tcaggcgggc acgctcgcgg gcaacccgct ggcgatgacg gcgggcattg agacgctgaa
     5881 ggaaatccag aagccgggaa tatgggagac gctcgaagca cgttcggcgc aactggcgat
     5941 aggtttgagc gcagcggcgg aagacgctag ggtccgcgtc gtgcttcagc gcgtcgggac
     6001 gatgttcacg gcgttcttca ccgagcgccc ggtcgtcaac tgggcgacag ccaaaacggc
     6061 cgacacgaaa cagtacgcag cgtacttccg cgccatgctg gaacgcggtg tatacttagc
     6121 gccgtcgcag ttcgaggctg ggtttatgtc ggcagcgcac ggtggcgaag agattgggct
     6181 gaccgtgcag gcggccgaga aggctctcaa gcaactcgcc gacctttgat agaggatacc
     6241 atcgctcatg cgtaggctca agggagatca tcatgtctac catgaaagac ggcgcagacc
     6301 tggaacagcc agggggcaca cctgaatcca gccagcgtga gtatccccat ggcccaattc
     6361 cttcggccca tgccatcgtc ttgcggaatg atagggtcct cctggtgaag cgtgcccacg
     6421 accccggcaa agggcgctgg agcctgccag gcgggatgat ccggcttgga gagacgatct
     6481 acgaggccgt ccagcgcgag gtgcgcgaag agtgcagcat ggaaatcgag cctgttcgca
     6541 tcctcgacgt gttcgacagt atcctcaaag atgaacaagg gcgcatccgc tttcattacc
     6601 ttgtggtgta tctgctggcc cgctacctga ctggagaggc gcaggcaggt tcggatgctt
     6661 cagaggtgcg ctgggcgacg cgcgaggaac tgaacgcgct cgacatgcac ccgcgcgttc
     6721 gagagacggt acacgcgacc tggctgagtt gatgctcgaa ggcgcaccat tcgctctcat
     6781 gcggatggcc ctgtcctgtt aggcgctgtg tgtgtccagc agaaatcgca caccgggccg
     6841 ccacgcccga gggtggtggt gcgcgcccat gcaatggaga gtggcaacgc ctcggcggcc
     6901 agatcgtcca atcgacagaa gtgttcggtc agctccggca caccataggc ggtgagcacc
     6961 ttcaggtaaa ggcaactgcg gatgttgaaa gccacccgcc gatcactgtc ttccacccac
     7021 tcgtaggccc acccttccgg cggaaaatcg cgcttcattc gctggcgcac ggctgcccgg
     7081 aagatggcaa acttccaggg cagatggccc aataacgcga gcgccttgcg catctcggag
     7141 ccttcggggg cgcccagcag atcatcccat tccgccagcg ctgcctgcag atcaccgtgc
     7201 gtctcgcaca ggatttggta gagagccaaa gctggcagga tgtagcgttc caggtgcatg
     7261 cgcagggcac gatgggtgaa gcgcggtcgg tgggcataca gttcctcgta gcgcgcctgc
     7321 actcgcgcag ccagcagcgc cgcatccacc tcacccaggc gcttggctaa caagcgttgc
     7381 cagagatcga ggcggtgcgc ccgcccgggc acggccggcc gccgcctgag ggccaggctg
     7441 cccagtgtgc ccagagttac gccgagcaag agtgcccatt gttcgttgcg ccggttggga
     7501 acgatgggtt tcatagcgtg ctcctttatt ggaatggaca atcactctga cttcaacaga
     7561 tccaggatgt cctccttctg agcctgctca gtttcttccg ccgtagcccg gtacacgaca
     7621 tcgtgcgggc gaacccgttt gcccaccatc agcgccacgt cttgccccgg ttgggcctca
     7681 tgcacgggtt gatgattaat ctgcaaagac gtgacctttt gatggaaatc ggtcttgtgg
     7741 ccgacaatat gcacgtagtc ctccactcga atgacatcgg tgagcgccag cacggccacc
     7801 ctgaggcggt cgtagtagtg ggtcacttga ccaatgcgca tgccagacat ggttcagacc
     7861 tcctttatct tgtcgagagc gtaaaacagc gccgtaactg actcc
//
LOCUS       DBNHXB010000021         6484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1179328, whole genome shotgun sequence.
ACCESSION   DBNHXB010000021 DBNHXB010000000
VERSION     DBNHXB010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1179328"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="AC162G_00765"
     CDS             <1..1343
                     /locus_tag="AC162G_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR5074378.1"
                     /translation="GGTDILIELDRRIRTPSALIDITRIPELDRIRLESGTFHLGPLV
                     THNHVVGSDLLVERAYPLARACWEVGAPQIRNRGTIAGNLITASPANDTITPLWALGA
                     SVTLKSHRGERTLPFEQFFHGVRKTAIAPDEMLTDISFPAMQPNERGVFVKLALRRTQ
                     AIAVVNIAILLGFEGNRIGKARITLGSVAPTIVRAPDAENFLEGKSLGDAAINAAGEL
                     ATAAARPIDDVRGTAEYRSEMIRVTTIRALRMLRDGTERADWPVAPAMLWGTTDGHFP
                     AGNLPGTHHAEDGEQPIEARINSKPYTIRGANDKTLLRMLREDAGLIGTKEGCAEGEC
                     GACTVFLDGIAVMACMVAAPRAHGSEIVTIESLARDGALHPVQQAFVAEDAVQCGYCT
                     PGFVMAGAKLLEERPYPTRYQIEQSISGNLCRCTGYFNILNAIERAARQRVDNG"
     gene            1355..2785
                     /locus_tag="AC162G_00770"
     CDS             1355..2785
                     /locus_tag="AC162G_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5074379.1"
                     /translation="MTADRSIRLSDGHTLPVWKKIAYGAGDSGFSLTSTALALVYLDF
                     LINVVGLDPQLAGISIGVGRVWDAFNDLFVGTLSDRTRSRWGRRRPYLLFGAIPFGVA
                     FVLMWLVPPANEQALLAMYYTAMYIVFDTTFTLVNVPYIALTPELAPTYDGRTSLHSY
                     RMAFSIGLGLAGAIAPLAIVDAVAGPGTSLEARQAAYALMSIVLAVASVIPIYITFAA
                     TRERPEYQALPMPALRESFRIAASNKAFLIAVGVYLLTWMPIDLIQFVLVFLIRDALG
                     LGSVERDTIFLLIFGVAILALPLWVWLSRQWDKKRAYQLGMAFLAAVLIALSFISPGM
                     VGAIYLLAVLAGIGLSAAHAIPLAILPDTIEWDELRTASRQEAAYYSVVTLIQKLAGA
                     ATIALTGTLLAASGYIQGARPDAIIAQPESALNAIRALTGPLPAAFFIAGILLVSFYP
                     ISRERHARIIKALDKKRELRKRSVSE"
     gene            2984..5227
                     /locus_tag="AC162G_00775"
     CDS             2984..5227
                     /locus_tag="AC162G_00775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR5074380.1"
                     /translation="MTESNVIGQSIPRTDAIAKVMGAALYPGDLAMPNMAHMKILFAG
                     RPHARIRRIDASKAEQAPGVIAVFTSKDVPVNEYGLILNDQPVLCGPLGEGATGAPGC
                     DVVRFVGDQVALVIAETEKQAAHARDLIEVDYEDLPVVADIRAAMRPGAPQLHPDKPG
                     NILNHYRIRKGDAGAAWAKCDAIVEAEYHTPPQEHAYLQPEAGLATLDEAGRVTVQVA
                     GQWTHEDRKQIAHALGLPEEQVRVIYPAIGGAFGGREDMSVQIVLALAAWRLHQRGID
                     RPVKIVWSREESIIGHHKRHPMIVKTKWGASKEGKVLAAEVQLYSDAGAYAYTSTKVQ
                     GNATLMCTGAYEIPNVKVDTYAVYTNNIPSGAFRGFGGPQGLYAAEMQMDKLALALGM
                     DPVEFRLKNVLREGSILSVGTPVPKGVSLETVIERCAEESGWTRTESGWQRPPRTSGR
                     HAHKKYGVGFACGFKNVGFSFGFAEHCAAKVELRGQAEIEEAIVYHAGADVGQGAHTA
                     MRQMAAEALDIPFERVRLVASDTATSGDSGSASASRMTFMAGNAILGAAEFAMKKWID
                     EERPAIGEYIYRPPKTTPFDPQTGQSMPNFTYGYVAETVEVEVDTETGHVRVLCVWCA
                     DDVGKAINPKLIRGQIEGAIVQALGYAIIENFQTQDGRVLTPYLSNYLIPTTLDAPQE
                     VHSVLVEERDSLGPWGARGMAEMPYLPFAPAVCAAVYDAIGVWFDEFPLTPDRVLKAI
                     RHHKKRG"
     gene            5483..>6484
                     /locus_tag="AC162G_00780"
     CDS             5483..>6484
                     /locus_tag="AC162G_00780"
                     /inference="COORDINATES: protein motif:HMM:NF015140.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HIR5074381.1"
                     /translation="MPSQAGSIQSSTRSGAAPIIAMLLALALALTVTVLVLAATDNNL
                     TSRLSIGDVSVVEGDGDGVEAVFTVTMSTAMTETVSVEYATADGTATAPADYTAIPTT
                     TLFFAPGETARTITVTVHGDTLIETDETFFVNLANTRNATIAVGQGVGTITNDDALPS
                     LSITDVSALEGNSSKSNVMFTVTLSAASDQQVTVGYASANGTATWPSDYFRIRPATLN
                     FPPGTITQTITAEIKGDTLYESDETFFVNLFNATNATLADDQAVGTLLNDDPEPTVQF
                     SAATYTVHEGAGSATITAALTGATAVTATVSYATSAASGTLAFAPGVTNQTFSVPIIK
                     "
BASE COUNT         1203 a         2096 c         2096 g         1089 t
ORIGIN      
        1 ccggcggaac cgacatcctg atcgagctgg atcgcagaat caggacgccg agcgcgctga
       61 tcgacatcac gcgcatcccc gagcttgacc gcatccggct cgaaagcggc acgttccacc
      121 tcggtcccct ggtgacgcac aaccacgtcg tcggatcgga tctgctggtc gagcgcgcgt
      181 acccgctggc gcgagcatgc tgggaggtcg gcgcgccgca gattcgcaac cggggcacga
      241 tcgccggcaa cctcatcacg gcctcgcccg ccaacgacac gatcactccg ctgtgggcgc
      301 tgggcgcgtc ggttaccttg aaaagtcacc gtggcgagcg gacgctgccc ttcgaacagt
      361 tcttccacgg cgtgcgcaaa acggctatag cgccggacga gatgctgacc gacatctcgt
      421 tcccggccat gcagcccaac gagcgcgggg ttttcgtcaa gctggcgctg cgccggacgc
      481 aagcgatcgc tgtcgtcaac atcgccatcc tactggggtt cgagggcaat cggatcggca
      541 aggcgcgcat cacgctggga tcggtcgcgc cgacgatcgt ccgcgcgccg gacgcggaga
      601 atttccttga gggcaagtcg cttggcgacg ccgccatcaa cgccgccggc gagctggcca
      661 cggccgctgc ccgcccgatc gacgacgtgc gcggcacggc cgaatatcgc agcgagatga
      721 tccgggtgac gacgatccgc gcgctgcgca tgctgcgcga cggaaccgag cgcgccgact
      781 ggccggtcgc gccggcgatg ctgtggggaa cgacggacgg ccactttccg gccggcaacc
      841 tgcccggcac gcaccatgcc gaggacgggg agcagcctat cgaggcgcgg atcaatagca
      901 aaccgtacac gattcgcggc gcaaatgaca agacattgct gcgcatgctg cgcgaagacg
      961 cgggactgat cggcacgaag gagggctgcg ccgagggcga gtgcggcgcg tgcacggtgt
     1021 tcctcgacgg catcgcggtc atggcgtgca tggtcgctgc gccgcgcgcg cacgggtccg
     1081 agatcgtcac gatcgagagc ctggcgcggg atggcgcttt gcatcccgtc cagcaggcgt
     1141 tcgtcgccga ggacgcggtg cagtgcggct actgcacgcc gggtttcgtg atggccgggg
     1201 ccaagctgct cgaagagcgc ccctatccca cccgttatca gatcgagcaa tccatcagcg
     1261 gcaatctgtg ccgctgcacg gggtacttta acatcttgaa tgcgatcgaa cgagcagcaa
     1321 ggcaacgggt tgacaacgga taaacggata caccatgacg gccgaccgtt ccatccgttt
     1381 atccgatggt catacgttgc ccgtttggaa aaagatcgcc tatggcgcag gcgacagcgg
     1441 cttctcgctg acgtcgaccg cgctggcgct ggtgtacctg gattttctga tcaacgtcgt
     1501 cgggcttgat ccacagctcg ccggcatctc gatcggcgtc gggcgcgtgt gggacgcctt
     1561 caacgatctg ttcgttggca cgctgagcga tcgcacgcgc agccggtggg ggcggcgcag
     1621 gccgtacctg ctgttcggcg cgatcccttt cggcgtcgcc ttcgtgctga tgtggctcgt
     1681 cccgcctgcg aacgagcagg cgctgctggc gatgtactac acggcgatgt acatcgtctt
     1741 cgacacgacg ttcacgctcg tcaacgtgcc atacatcgcg ctgacgcccg agctggcgcc
     1801 cacgtacgac gggcgcacct cgctgcactc gtaccgcatg gcgttctcga tcgggctggg
     1861 gctggccggc gcgatcgcgc cgcttgccat cgtcgacgcc gtcgcggggc cgggcacgtc
     1921 gctcgaggcg cggcaagcgg cctacgcgct gatgtcgatc gtcctcgccg tcgccagcgt
     1981 catcccgatc tatatcacgt tcgccgcgac gcgcgagcgg ccggagtatc aggccctgcc
     2041 gatgccagcg cttcgcgaat cgttccgcat cgccgcgagc aacaaggcgt tcctgatcgc
     2101 cgtcggcgtc tacctgctca cctggatgcc gatcgacctg atccagttcg tgctcgtatt
     2161 cctcattcgc gacgccctcg gtttgggcag cgtcgagcgc gacacgatct tcctgctgat
     2221 cttcggggtc gccattctgg cgctgccgtt gtgggtgtgg ctatcgaggc aatgggacaa
     2281 gaagcgcgcg taccagttgg ggatggcctt tctcgccgcc gtgctcatcg cattgagttt
     2341 catctcgccc ggcatggtcg gcgcgattta tctgctcgcc gtgctggctg gcatcggcct
     2401 ttcggcggcg cacgcgatcc cactggcgat cctgccggac acaatcgagt gggacgagct
     2461 gcgcacggcc agtcgccagg aagcggccta ttacagcgtc gtgacactga ttcagaagct
     2521 tgccggcgcg gccacgatcg cgctgaccgg gacgctgctg gccgcctccg gctacattca
     2581 gggagcaagg ccggatgcga tcatcgcgca gcccgaatcg gcgcttaacg ccatccgcgc
     2641 gctcaccggc ccgctgccgg ccgcgttctt catcgcgggg atcttgttgg tatcgttcta
     2701 cccgatctcg cgcgagcgac atgcaaggat cataaaggcg ctggataaga aacgggagtt
     2761 gaggaaacga tccgtgagcg agtgagtatg attgggaatg gtgctgatga tcttgaccgg
     2821 ccagaccacg tgggggtgct ggctatcgtg cctgagcctg ccggccacgg cgctgttcct
     2881 gtggatcgcg ctgcgagcga tacccaaatg agagaaatga aggtttacct ggagaagagg
     2941 tgaacgacac cattaagatg ccagtcacct gaacgaggtc aagatgaccg aatcaaacgt
     3001 gatcggacaa agcattccac gcaccgacgc catcgccaaa gtcatgggcg cggcgctgta
     3061 tcccggcgat ctcgccatgc cgaacatggc gcacatgaaa atcctgtttg ccgggcggcc
     3121 gcatgcccgc atccgccgca tcgacgcgtc gaaagccgag caggcgcccg gcgtgattgc
     3181 cgtgttcacg tcgaaggatg tgccggtgaa cgagtatggc ctgattctca acgatcagcc
     3241 ggtcttgtgc ggcccgctcg gagagggcgc gaccggcgca ccgggctgcg acgtcgttcg
     3301 cttcgtcggc gaccaagtcg cgctggtgat tgccgagacc gaaaagcagg cggcgcacgc
     3361 gcgcgatctg atcgaggtcg attacgaaga cttgccggtc gtcgccgaca tccgcgcggc
     3421 gatgcggccc ggcgcgccgc aattgcatcc cgacaagccc ggcaacattc tgaatcacta
     3481 ccgcattcgc aagggcgacg ccggtgcggc gtgggcgaag tgcgatgcca tcgtcgaagc
     3541 cgaatatcac acacccccgc aggagcacgc ctatctccag cccgaggccg ggctggccac
     3601 tctcgacgaa gcgggccggg tgacggtgca ggtggccggg cagtggacgc acgaagaccg
     3661 caagcagatc gcgcacgcac tcggcctgcc ggaggagcag gtgcgcgtca tctatccggc
     3721 gatcggcggc gcattcggcg ggcgcgagga tatgtcggtg cagatcgtgc tcgcgctcgc
     3781 cgcgtggcgg ctgcatcagc gcggcatcga ccggccggtc aagatcgtgt ggagccgcga
     3841 ggagtcgatc atcggccatc acaagcgcca cccgatgatc gtcaagacga agtggggcgc
     3901 gagcaaggaa gggaaggtcc tcgccgccga agtgcaacta tacagcgacg cgggcgcgta
     3961 cgcctacacc tcgaccaagg tgcagggcaa tgccacgctg atgtgcaccg gcgcgtacga
     4021 aatcccgaac gtcaaggtgg atacctacgc ggtgtacacg aacaacatcc cgagcggcgc
     4081 gttccggggc ttcggcgggc cgcaggggct gtatgctgcc gagatgcaga tggacaagct
     4141 ggcgctggct ctgggtatgg acccggtcga gttccgcttg aagaacgtct tgcgcgaggg
     4201 atcgatcctc tccgtcggca cgccggttcc aaaaggcgtc agcctggaga cggtcatcga
     4261 gcgctgcgcg gaggagtctg gctggacgcg gaccgaatcc ggctggcagc gcccgccgcg
     4321 cacgtcgggc cgccatgcgc acaagaaata cggcgtcggc ttcgcctgcg gcttcaagaa
     4381 cgtcggattc tcgttcgggt ttgcggagca ctgcgcggcg aaggtcgagc tgcgcggcca
     4441 ggccgagatc gaagaggcca tcgtgtacca cgccggcgcc gacgtcggcc agggcgcgca
     4501 caccgccatg cgccagatgg ccgccgaggc gctggatatc ccgttcgagc gggtgcggtt
     4561 ggtggcgtcg gacacagcga cgagcggcga ctcgggcagt gcgtcggcgt cgcgcatgac
     4621 gttcatggcc ggcaatgcca tcctcggcgc ggccgagttc gccatgaaga aatggatcga
     4681 cgaagagcgg ccggccatcg gcgagtacat ctatcgcccg ccaaagacga cgccgttcga
     4741 tccgcagacc ggccagagca tgccgaactt cacgtacggc tacgtcgccg agacggtcga
     4801 ggtcgaggtc gataccgaga cggggcacgt ccgcgtgctg tgcgtatggt gcgccgacga
     4861 cgtgggcaag gcgatcaacc cgaagctgat ccgggggcaa atcgagggcg cgatcgtcca
     4921 ggcgctgggc tacgcgatca tcgagaactt ccagacgcag gacggccggg tgctgacgcc
     4981 gtacctgagc aactacctga tcccgaccac cctcgacgcg ccgcaggaag tgcactccgt
     5041 cctggtcgag gagcgtgatt cgctcggccc gtggggggcg cgcggcatgg ccgagatgcc
     5101 atacctgccc ttcgcgcccg ctgtatgcgc agcggtgtac gacgcgatcg gcgtctggtt
     5161 cgacgagttt ccgctgacgc cggatcgagt gttgaaggca atccggcacc acaagaagcg
     5221 aggataaccc cggatccgac aggatctcga ccagtgtccg cctaaaatgc ggtaatgacc
     5281 gctccagaca cagaacccat ctgcctcctc gccggctcca tgatttgcaa acccagtaca
     5341 gacttagttc taactttttc gatccaattt tctaacttct ttggctcaaa ctagagcaaa
     5401 gcttccactt tgtcgaaagc cagctcatag actgtcaagc cataggtcgt ggcagctatc
     5461 gcacgtcgtc acggaggatt tgatgccatc ccaggcaggc tcgatacagt ccagtacacg
     5521 gtctggcgct gcgccgatta tcgccatgct gctggccctg gcgctcgccc tgacggtcac
     5581 cgtgctggtg ctggccgcga ctgacaacaa cctcacgtcg aggctgtcga tcggtgacgt
     5641 gagcgtcgtc gagggagacg gcgacggcgt cgaagcggtt ttcaccgtga cgatgtcgac
     5701 ggccatgacg gagaccgtgt cggtcgagta cgccaccgcc gacggcacgg ccaccgcgcc
     5761 ggcagactac accgccatcc ccacgacgac attgttcttc gcgccgggcg agacggcacg
     5821 cacgatcacc gtgacggtac acggcgacac cttgatcgag actgatgaga cattttttgt
     5881 caacctagcc aacaccagaa atgcaacgat cgctgtcggc cagggtgtgg gcacgatcac
     5941 gaacgacgat gcgctaccca gcctgtcgat taccgacgtg agcgccctcg aggggaatag
     6001 cagcaagtcc aacgttatgt ttaccgtcac gctctcggct gcgagcgacc agcaagtgac
     6061 ggtgggctac gcttctgcca acggcaccgc cacctggccg tccgactact ttcgtattag
     6121 gccggcgacg ctgaacttcc ctccgggcac gatcacccag accatcacag ccgagatcaa
     6181 gggcgacacc ctgtacgaga gtgatgagac cttcttcgtc aacctgttca acgcgaccaa
     6241 cgcgacgctc gccgacgacc aggctgtcgg cactctcctc aatgacgacc cggagcctac
     6301 cgtccagttc agcgccgcca cctacactgt gcacgaaggc gccggctcag cgacgatcac
     6361 cgcggccctc accggcgcga cggccgtgac ggccacggtg agctacgcca cgagcgccgc
     6421 cagcggcacg ctggcattcg cacccggcgt aaccaaccag acattcagcg tgccgatcat
     6481 taag
//
LOCUS       DBNHXB010000022         4907 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2689157, whole genome shotgun sequence.
ACCESSION   DBNHXB010000022 DBNHXB010000000
VERSION     DBNHXB010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2689157"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="AC162G_00785"
     CDS             complement(<1..344)
                     /locus_tag="AC162G_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012447.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HIR5074382.1"
                     /translation="MRRIDGDMPHTALAIDGASLTIENVMKVARDAQPVALRDEARSR
                     VAQSRAWVDRVVAEGSPTVYGVNTGFGVFASRQVAREDAERLSRNLILSHAVGVGDPL
                     PEDVVRAAMLIRA"
     gene            complement(331..1068)
                     /locus_tag="AC162G_00790"
     CDS             complement(331..1068)
                     /locus_tag="AC162G_00790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5074383.1"
                     /translation="MRKRLVRFGLQLLYNELAWTYDFVAGCVSLGNWKAWGRTSIRYL
                     RGKRILELAHGPGHLLAALKQASYEPVGIDLSPRMSRQAARGLRRAGIDVPLVRCRAE
                     ALPFRSGAFDAAVATFPTTFILDPRTLRDIARVTDRAGRLVIVAAASLGGRGLLSKPI
                     EALYEVTGQSEPMPHGNELAFVQAGWAIQTAYEAVGGSNVFLVIGDKVDRHFGSAGQP
                     VEKVGSRDIPLTTGEKSTTKDAASAEN"
     gene            complement(1121..1975)
                     /locus_tag="AC162G_00795"
     CDS             complement(1121..1975)
                     /locus_tag="AC162G_00795"
                     /inference="COORDINATES: protein motif:HMM:NF016668.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HIR5074384.1"
                     /translation="MTKKLIVNADDFGRTHRVSEGILTAHREGIVTSTTAMMNIPGAA
                     HDLHRALAEAPNLGLGVHLVFTLGRPLLPPEWASTLIDEHGHFLAQEAILEKPERLDP
                     DELLSEFKAQVSAFEHSVGRRPDHVDCHHFAHVHPHLFAVYHNLALELGLPMRIPFPR
                     TEAGLADPALMPGMASAIPEATVKAMARANWARLAERPVRAPDHFIVGFYGDHVSVEY
                     LLSLLDRLPDGISELMAHPGYSDETLAAESAYSARREDEIAALTDPRVRQRIADLGIE
                     LTTYASVA"
     gene            complement(1944..2762)
                     /locus_tag="AC162G_00800"
     CDS             complement(1944..2762)
                     /locus_tag="AC162G_00800"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR5074385.1"
                     /translation="MNAFLAERDRPLNLAHRGASAYAPENTLAAFRLAAEMGADGLEI
                     DAKLSRDGAIVILHDATVDRTTSGSGRVSDLTLSQLKSLDAGSKFRSRFAGEHVPTLD
                     EVIDAVGDRLIINIELTNYTSRRDGLEERVVEFVNRNGLVQRVLISSFSPLSLRRVKR
                     AAPHLVCGLLYPAGGAVFFRRAWLACIVPGLEARHPHFSLVDAASVRRFHRRRQKVNT
                     WTVNEEADMRRVITAGVDAIMTDKPDVLQRIVNPRASRDRQEAQHDQETHRQRR"
     gene            complement(2944..4191)
                     /locus_tag="AC162G_00805"
     CDS             complement(2944..4191)
                     /locus_tag="AC162G_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR5074386.1"
                     /translation="MPRSIAESGLNMGFLSDLVLKVMYFEGYLSGSQIAERIRLPFSG
                     IVDQVLEFLKREQLTEVKGQGGFGEQAYQYAITLKGRDKAHEALDRSHYAGAAPVTLD
                     TYQKAMHRQSMQEFTVHQRMMRQGLAHLVINERTFGQIGPAVNSARSIFLYGPPGNGK
                     TAIAESVGRLILAENIYVPHAVEVDGQVIKVFDSVNHQRAGEGNGQSRGRSIDPRWVQ
                     IRRPVIITGGELTLETLDLVWDEVNKYYEAPFQMKANGGLFLIDDFGRQQVRPRDLLN
                     RWILPLEKRVDYLTLHTGRKIEIPFDVLIVFSTNLAPRDLVDEAFLRRIRHKIEIGDP
                     SFEEYREIFKRVCQEKNVPYDDRALAYLLQEWYIKPRRKMRAVHPRDLIEQLVDIARY
                     MNTQPLLSKDLIDRACEAYFVEI"
     gene            4475..4894
                     /locus_tag="AC162G_00810"
     CDS             4475..4894
                     /locus_tag="AC162G_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR5074387.1"
                     /translation="MYQQIVIVGNLGRDPEMRYTPSGTPVTSFSVATSRKYTGSDGQQ
                     VKETAWFRVSVFGKQAEACAQYLKKGSACLVEGRLNPDLQTGGPRMWTRQDGTTGASF
                     EVHANTVRFLSARGEGAAPTAESVDEAPAGGEDEIPF"
BASE COUNT          873 a         1511 c         1599 g          924 t
ORIGIN      
        1 gcgcggatca gcatcgcggc gcggacgacg tcttcgggca gcggatcgcc gacgcccacg
       61 gcgtggctga ggatcaggtt gcgtgacagg cgttccgcgt cttcgcgcgc gacctggcgg
      121 ctggcaaata cgccgaagcc ggtgttgacg ccgtatactg tcggtgatcc ttcggccacg
      181 acgcgatcga cccaggcgcg cgactgcgcg acgcgtgagc gggcttcgtc gcgcagcgcg
      241 accggctgcg cgtctcgcgc aaccttcatg acgttttcaa ttgtgagcga tgcgccgtcg
      301 atggccaggg cagtatgggg catgtcgccg tcaattctcc gcactcgccg cgtcctttgt
      361 ggttgatttt tctccggtgg tcaggggtat gtcgcggctg ccaacctttt ccaccggttg
      421 accggcactt ccaaagtgcc ggtcaacttt gtctcctatg accaggaaca cgttactgcc
      481 gccaactgcc tcgtaagcag tttgtatggc ccaacccgcc tgcacgaatg ccaattcgtt
      541 gccgtgcggc atcggctcgc tctggccggt gacctcatac aaggcctcga tcggcttcga
      601 cagcagccca cgcccgccca ggctggcagc ggcgacaatg accagacggc ccgctcgatc
      661 ggtcacccgc gctatgtcgc gcagcgtgcg tggatcgaga atgaaagtcg tcggaaaggt
      721 tgccacggct gcgtcgaagg cgcccgatcg gaacggcagc gcctctgcgc ggcagcggac
      781 gagcggcacg tcgatacctg cgcggcgcag cccgcgcgcg gcctgccggc tcatgcgtgg
      841 tgacaggtcg atgccgaccg gctcgtagct cgcctgcttc aaggcggcca acaggtggcc
      901 cgggccgtgc gccagctcca gtatccgctt gccgcgcaga tacctgatcg acgtgcggcc
      961 ccaagccttc cagttgccga gcgacacgca cccggcgacg aaatcgtacg tccaggccag
     1021 ctcattgtac aacaactgca ggccgaagcg aacgaggcgt ttccgcatgg gagtgatcag
     1081 tgacacgtga tcacgtgatc acgtaatgac gtggttctga tcacgcgaca gaggcatacg
     1141 tcgtcagctc gatgccgaga tcggcgatgc gctgccgcac gcgcggatcg gtcagcgcgg
     1201 cgatctcgtc ttcgcgcctg gcgctgtaag cggattcggc ggcgagcgtt tcgtcgctgt
     1261 agccagggtg agccatcagc tcgctgatcc catccggcag ccggtcgagc aggctcagca
     1321 gatactcgac cgatacgtga tcgccgtaga agccgacgat gaagtgatcc ggcgcgcgca
     1381 cgggcctctc tgccaggcgg gcccagttgg cccgtgccat ggctttcacc gtcgcctctg
     1441 ggatcgccga ggccataccg ggcatgagtg ccgggtcggc caggccagct tcggtgcgag
     1501 ggaaagggat gcgcatcggc aggcccagct ctaacgccag gttgtgatac acggcgaaca
     1561 gatgcgggtg gacgtgcgcg aagtggtgac agtcgacgtg gtccggccgg cggccgacgc
     1621 tgtgctcgaa cgcgctgacc tgggctttga attcggagag cagctcgtca ggatcgaggc
     1681 gctctggctt ctcgaggatg gcctcctgcg cgagaaagtg gccatgctcg tcgatgagcg
     1741 tcgacgccca ttccggaggc agcagaggcc gccctaacgt gaacacgagg tggacgccca
     1801 gcccgaggtt gggcgcttcg gccagtgcgc ggtggaggtc gtgcgccgcg ccgggtatgt
     1861 tcatcatcgc cgtcgtgctg gtgacgatgc cttcccggtg cgccgtcaag atgccttcgc
     1921 tgacacgatg cgtccggcca aagtcatctg cgttgacgat gagtttcttg gtcatgttgt
     1981 gcctcttgcc tatcgcgcga agcgcgcggg ttgactatcc gctgcaggac gtccggcttg
     2041 tcggtcataa tcgcatcgac gcccgcggtg atcaccctgc gcatatcggc ttcctcgttc
     2101 accgtccagg tattgacttt ctgccgacgc ctgtgaaatc tccgcacgga cgccgcatcg
     2161 accagtgaga agtgcgggtg ccgtgcctcg aggccgggga cgatgcacgc gagccaggcg
     2221 cggcggaaga agacggcgcc tccggccggg tagagcagcc cgcacaccag gtgcggcgcg
     2281 gcgcgcttca cccggcggag cgacaagggg ctgaacgaag aaatcagtac acgctggaca
     2341 aggccgttgc gattgacgaa ttccacgacc cgctcttcca atccatcgcg gcggctggtg
     2401 tagttcgtca gctcgatatt gatgattaag cgatcgccga cggcatctat gacctcgtcg
     2461 agggttggga catgctcgcc ggcgaatctc gaacgaaact tcgacccggc gtccagagac
     2521 ttgagctggc tgagcgtgag atcggacaca cgtcccgacc cgctcgtcgt ccggtcgaca
     2581 gtcgcgtcgt gcagaatgac gattgcgccg tcacggctga gcttggcgtc gatctcgagg
     2641 ccgtcggcgc ccatctcggc agccagccga aacgcggcga gcgtgttttc cggcgcgtag
     2701 gctgatgcgc cccggtgggc gagattgaga gggcgatctc tctcggccag aaacgcgttc
     2761 atcgcgcggc gtgaaccacg aagcgtaact gctcagcccg cgtcattgcg agcgcttttt
     2821 gcgaagcaat ctccggcacg gaccgccctg cgcgctcgtg gcggctgagc agtcaccccg
     2881 aaggcacggc gactcgaaaa tgatacgcgt ctgtgcgcct tcgcgagcat ggccactatc
     2941 cgcctagatt tccacaaaat acgcctcgca ggcgcggtcg atcaggtcct tgctcaacaa
     3001 gggctgcgtg ttcatgtagc gcgcgatgtc gaccagttgc tcgatcaggt cgcgggggtg
     3061 gacggcgcgc atcttgcggc gtggcttgat ataccactcc tgcagcaggt atgccagcgc
     3121 gcgatcgtcg tacggcacat tcttctcctg gcatacgcgc ttgaagattt cacggtattc
     3181 ttcgaacgag ggatcgccga tctcgatttt gtgacggatg cggcgcagga acgcctcgtc
     3241 gaccaggtcc cttggcgcca ggttggtcga gaacacgatc agcacgtcga acggaatctc
     3301 gatcttgcgg ccggtgtgca gcgtcaggta gtcgacgcgc ttttccagcg gcaggatcca
     3361 gcggttgagc aggtcgcgcg ggcgcacttg ctggcggccg aagtcgtcga tcaggaacaa
     3421 gccgccgttg gctttcatct ggaacggcgc ctcgtaatac ttgttgacct cgtcccatac
     3481 caggtcgagc gtctccagcg tcagctcgcc gcccgtgatg atgaccggac ggcggatctg
     3541 cacccagcgc gggtcaatgc tgcggccgcg cgactgcccg ttgccttcgc cagcgcgctg
     3601 gtggttgacg ctgtcgaaga ccttgatgac ctgcccatcg acctcgacgg cgtggggcac
     3661 gtagatgttc tccgcaagga tcaggcggcc gacggattcg gcaatcgccg tcttgccgtt
     3721 gccgggcggc ccgtacagga agatcgacct ggcagagttg accgccgggc cgatctgacc
     3781 gaaggtgcgc tcgttgatca ccaggtgggc caaaccctgg cgcatcatgc gctggtgaac
     3841 cgtgaattcc tgcatcgact ggcggtgcat ggccttctgg taggtgtcga gcgtgaccgg
     3901 cgccgccccc gcgtaatggc tgcggtcgag cgcttcgtgg gccttatcgc gtcccttcag
     3961 tgtgatggcg tactgatagg cttgctcccc gaatccgccc tgacctttca cttccgtcaa
     4021 ttgctcgcgt ttcagaaact ccaggacctg gtcgacgatc ccgctgaacg gtaggcgaat
     4081 gcgctcggcg atctgagagc ctgagaggta tccttcgaaa tacatgacct tcaggacgag
     4141 atcggagagg aagcccatgt tcaggccgga ctcggcgatc gatcgcggca tgggaggctc
     4201 gtatggcagt ggcttcacgg taagggtggg ggcgggttgg ggctgcgctt tcaattcgtc
     4261 tgcctcgctt cgggttggcc ggggtgaaac aacacaatcg gcggtggcat ggcccaggtg
     4321 aatgtggttc ttgcgaagac cgttgcgatt ataccataag gtagggggct tctgaccgtt
     4381 aagaactcgt tgacaatgca tttaacttgc ggtaaactat cggcacaaat gttctgttgt
     4441 gggcgtccat accataccca agaggaggca tctcatgtat caacagatcg tcatcgtcgg
     4501 caacttggga cgcgatcctg aaatgcgcta cacccctagc ggcacaccgg tcaccagctt
     4561 cagcgtggcg acaagtcgta agtacaccgg cagcgatgga cagcaggtca aggaaaccgc
     4621 atggtttcgg gtgagtgtat tcggcaaaca ggccgaagcc tgcgcgcaat accttaagaa
     4681 aggcagcgcc tgtctcgtgg agggacgcct gaaccccgat ctgcagacgg gcggcccacg
     4741 catgtggacc cgccaggacg ggacgaccgg tgcatcgttc gaggtgcacg ccaacaccgt
     4801 tcgattcctg agcgcccgag gcgagggtgc ggcgcccacg gcggaaagcg tcgacgaggc
     4861 gcccgccggc ggcgaagacg aaatcccctt ctgagccact ctcgccg
//
LOCUS       DBNHXB010000023        22274 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1011939, whole genome shotgun sequence.
ACCESSION   DBNHXB010000023 DBNHXB010000000
VERSION     DBNHXB010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 22274)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22274)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22274
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1011939"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="AC162G_00815"
     CDS             <1..302
                     /locus_tag="AC162G_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR5074388.1"
                     /translation="PGSIRPHTTFDAEVYVLKKEEGGRHKAFFPGYRPQFYIRTMDVT
                     GEIALPAGVEMVMPGDNVNMTVKLIMPVALEAGSHFAIREGGLTVGAGVITKVYE"
     gene            367..675
                     /gene="rpsJ"
                     /locus_tag="AC162G_00820"
     CDS             367..675
                     /gene="rpsJ"
                     /locus_tag="AC162G_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR5074389.1"
                     /translation="MVKQRIRIRLKAYDHRLLDQSAKRIVEAAERTGATTVGPVPLPT
                     RIERFTVQRGAFIDKNSQEHFEIRTHKRLIDVVDPDSKTIDTLMRLNLPAGVDIEIKF
                     "
     gene            773..1450
                     /gene="rplC"
                     /locus_tag="AC162G_00825"
     CDS             773..1450
                     /gene="rplC"
                     /locus_tag="AC162G_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR5074390.1"
                     /translation="MMKGILGRKIGMTQIFAENGERVPVTVIEAGPCTVTQVRTVKEN
                     GYSAIQIGFDPAKKAQRLSRGERGHLGLLKPDEKHPKRRKFPTDVPPLKSLREIRVRE
                     DEQYTEGQIIKADIFEVGERVDVIGTSKGRGFAGTVKRHGFSGGSKTHGQSDRMRAPG
                     SIGAGTTPGKVIKGKKMAGHMGTDRVTAQHLDVILVDAERNLIGVRGSVPGANGDLVL
                     VRETRKK"
     gene            1560..2183
                     /gene="rplD"
                     /locus_tag="AC162G_00830"
     CDS             1560..2183
                     /gene="rplD"
                     /locus_tag="AC162G_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR5074391.1"
                     /translation="MMKVPVKNMAGEHVGEVELNKLIFEAPINTALMHQALTRQLANA
                     RQGTAKTKTRGEVAGSTRKVWRQKHTGRARQGSRKAPQWRKGGTVFGPSPRSYRQRMP
                     KNMRHAALRSALSAKAADHQIVVLDALMLDAPKTKAMADMLRNLEAGRSALILLSEGS
                     PTVERSIRNLEGVQYLRAQYLNVRDLLGADTVVVPQSALAAIDELLG"
     gene            2282..2593
                     /gene="rplW"
                     /locus_tag="AC162G_00835"
     CDS             2282..2593
                     /gene="rplW"
                     /locus_tag="AC162G_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR5074392.1"
                     /translation="MQVYDVLKRPLLTEKMTYLRDLNQYAFEVDGRANKEQIKQAVEA
                     IFKVDVVAVRIMHQPAKRRRHLRSRQIGKKAKQAVRHPGWKKAIVKLAANQRIELFEG
                     V"
     gene            2738..3574
                     /gene="rplB"
                     /locus_tag="AC162G_00840"
     CDS             2738..3574
                     /gene="rplB"
                     /locus_tag="AC162G_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR5074393.1"
                     /translation="MAIKKYKPTSPGRRGMTGDTFEDITKTKPERSLTVSLKKHSGRN
                     VQGRVTVRHRGGGAKRRYRLIDFRRDKHGMAAKVAAIEYDPGRSARIALLHYADGEKR
                     YIIAPSGMKVGDTVFSGPGSDVRVGNCLPLKDIPLGTLVHNVELYPGQGAQFVRSAGT
                     HAQVLARDAGYVTLRMPSGETRKVLDSCFAVIGQVGNLDHANVKLGKAGRKRHMGIRP
                     AVRGTAMSPRDHPHGGGEGRSPVGMPGPKSPWGKPTRGARTRVNKRTNRLIVRRRVSK
                     RR"
     gene            3650..3934
                     /gene="rpsS"
                     /locus_tag="AC162G_00845"
     CDS             3650..3934
                     /gene="rpsS"
                     /locus_tag="AC162G_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR5074394.1"
                     /translation="MSRSLKKGPFIDPKLLRKVEAMNRSGDKKVLRTWSRASTIFPQM
                     VGHTIAVHDGRRHVPIYITENMVGHKLGEFAPTRTYRGHSTREKTTEVRG"
     gene            3950..4288
                     /gene="rplV"
                     /locus_tag="AC162G_00850"
     CDS             3950..4288
                     /gene="rplV"
                     /locus_tag="AC162G_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR5074395.1"
                     /translation="MAFEARCATRFVPLSPRKVRLVADQVRGLQVDDALALLKFMPMA
                     AAAPVSKAIASAVANGEENLGLARDDLFVKEIRADEGPRRQWRRFGARGRFKPITRRS
                     SHITVVLSER"
     gene            4349..5014
                     /gene="rpsC"
                     /locus_tag="AC162G_00855"
     CDS             4349..5014
                     /gene="rpsC"
                     /locus_tag="AC162G_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR5074396.1"
                     /translation="MGRKVHPYGFRLKVVRDWQGRWFGKRGGPYLAMLSEDLALRKRI
                     MAKVSRSGVSRIEIERFPNQLIITIHTAKPGIIIGRKGAQVKELRESLEAMTGKKVKL
                     EVEEVTKPDLEASLVAENIAGQLERRISHGRAMKRAIQQAMRAGAEGIKITVAGRLGG
                     SEMSRTETVMEGRVPRSTLRADLDYARAEALTTAGRIGVKVWIYRGEILPEARAEDLA
                     TAH"
     gene            5047..5487
                     /gene="rplP"
                     /locus_tag="AC162G_00860"
     CDS             5047..5487
                     /gene="rplP"
                     /locus_tag="AC162G_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR5074397.1"
                     /translation="MLMPKRVKFRKQQRGRMKGKELRGAEVSFGEYGLQALEPCWMTA
                     RQIEAARRAVVHFAKRRGKLWIRVFPDKPVTQKAAETRMGKGKGAVDHWVAVVKPGRV
                     ILEVGGMDSGVAREALRLAAHKLPIKTQVITRRDWVAAEEGGAK"
     gene            5484..5735
                     /gene="rpmC"
                     /locus_tag="AC162G_00865"
     CDS             5484..5735
                     /gene="rpmC"
                     /locus_tag="AC162G_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR5074398.1"
                     /translation="MKVSEIRALRTEEIQSKMDEAHKSLFNMRFQAAVGQLEDFNRMR
                     AIRRDIARMKTILRERELAAQGRSPLLSSATGEGPGVRE"
     gene            5732..5989
                     /gene="rpsQ"
                     /locus_tag="AC162G_00870"
     CDS             5732..5989
                     /gene="rpsQ"
                     /locus_tag="AC162G_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR5074399.1"
                     /translation="MTEQQRKRLVGRVTSDKMDKTVVVTVQRLQRHPLYKKVIRRDRR
                     FMAHNEGNQAREGDLVRIVESRPLSRHKRWVIEEVIEKARG"
     gene            5994..6365
                     /gene="rplN"
                     /locus_tag="AC162G_00875"
     CDS             5994..6365
                     /gene="rplN"
                     /locus_tag="AC162G_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR5074400.1"
                     /translation="MVQHETRLNVADNTGAREILCMHVLGGSTRRYGHVGDVIVAAVK
                     QAAPNGSVKKGEVVRAVIVRTAKEYARPDGSYIRFDDNAAVIIDPESLNPKGTRIFGP
                     VARELRDKGFMKIVSLAPEVL"
     gene            6414..6734
                     /gene="rplX"
                     /locus_tag="AC162G_00880"
     CDS             6414..6734
                     /gene="rplX"
                     /locus_tag="AC162G_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013921150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR5074401.1"
                     /translation="MARIKKGDTVIVIAGENKGTQGTVQRVDAKTQRVVVTGVNIVKK
                     AQRPVRAGRGQTQTGIIEFEAPIHISNVMLVDPKSSEPTRVGHTVDASGAKRRVTRKS
                     GTEI"
     gene            6742..7284
                     /gene="rplE"
                     /locus_tag="AC162G_00885"
     CDS             6742..7284
                     /gene="rplE"
                     /locus_tag="AC162G_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR5074402.1"
                     /translation="MANLKKRYREEIVPALMRDFGYKNVMQAPRIQKVIVNIGAGDAL
                     ENAKTLDGAVADLRLITGQQPVITKARKDIANFKLRAGRSIGVKVTLRGERMWSFLDV
                     LANLALPRIRDFRGISPDAFDGRGNYTLGLREQIMFPQIQYDKIDKLRGMEITIVTTA
                     RSDEEGRRLLHLIGMPFRSN"
     gene            7287..7469
                     /locus_tag="AC162G_00890"
     CDS             7287..7469
                     /locus_tag="AC162G_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR5074403.1"
                     /translation="MAKKSMINRESRRKYPTRVRNRCRQCGRPRGYIRRFGLCRICFR
                     ELALRGVIPGVAKASW"
     gene            7590..7997
                     /gene="rpsH"
                     /locus_tag="AC162G_00895"
     CDS             7590..7997
                     /gene="rpsH"
                     /locus_tag="AC162G_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR5074404.1"
                     /translation="MNISDPISDMLTRVRNAIAVRHASVALPSSQIKIAIATILREEG
                     YIQDFEVTESGKRKTLRLWLKYTGERRDRQPAISGLQRVSRPGRRVYTSRDSIPWVLS
                     GMGIAILSTPKGVITGQQARRLNVGGEVLCYVW"
     gene            8021..8566
                     /gene="rplF"
                     /locus_tag="AC162G_00900"
     CDS             8021..8566
                     /gene="rplF"
                     /locus_tag="AC162G_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004695131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR5074405.1"
                     /translation="MSRIGRKPVPIPSGVAVTIDGPRVTVKGPRGELTRAFHPDMAIA
                     MDDGTLVVSRPSDARQHRALHGLTRALLANMVTGVTQGFRKTLVVEGVGYRADLTGKV
                     LLLHLGYSHPIRVEPPAGVKFSADSKARTITVEGADREQVGQIAALIRKLRPPEPYKG
                     KGIRYQGEVIRRKAGKAGKVG"
     gene            8604..8945
                     /gene="rplR"
                     /locus_tag="AC162G_00905"
     CDS             8604..8945
                     /gene="rplR"
                     /locus_tag="AC162G_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR5074406.1"
                     /translation="MRLRRQKRVRRGVNGTAGRPRLNVFRSLEHIYAQVIDDEQGRTV
                     VSASTIDRELRAEIASLSKTEQARRVGAALARRAQAAGVKQVVFDRGGYRYHGRVKAL
                     ADASREVGLEF"
     gene            8945..9511
                     /gene="rpsE"
                     /locus_tag="AC162G_00910"
     CDS             8945..9511
                     /gene="rpsE"
                     /locus_tag="AC162G_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR5074407.1"
                     /translation="MAKRRVSTERRDREVFEEPREELDERVVDIARVAKVIKGGRRFA
                     FRVVVVAGDNRGNVGVGVGKSRAVPDAIRKASERARSAMRPVVLEGTTIPHPVMAKSG
                     GARVMLRPASPGTGVIAGGGVRAVLEAAGVHDILTKSMGSSNKLNVIRATLAGLRQLK
                     SPDAVAQERGKPITDVAPFWRRTHVKQP"
     gene            9627..9848
                     /gene="rpmD"
                     /locus_tag="AC162G_00915"
     CDS             9627..9848
                     /gene="rpmD"
                     /locus_tag="AC162G_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR5074408.1"
                     /translation="MRITLVKSPTGYDEHQKRTVRALGLRRLRQTVEQADTPVVRGMI
                     AKIPHLVSVEPAPSEARPERSRRVEGEVK"
     gene            9845..10315
                     /gene="rplO"
                     /locus_tag="AC162G_00920"
     CDS             9845..10315
                     /gene="rplO"
                     /locus_tag="AC162G_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006093108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR5074409.1"
                     /translation="MKLHELKPVEGSKKRRKRVGRGLGSGSGTYAGRGRKGQHARQGG
                     VKGPYFEGGNLPLVRKLPFARGVGFTSRGKIHYVPINLSDLAALASRSPEWSEGEPVT
                     PEVLHRHGLLKSADDPVVILAYGEIERAVTVRAHRFSASARRKIEAAGGTVEML"
     gene            10324..11658
                     /gene="secY"
                     /locus_tag="AC162G_00925"
     CDS             10324..11658
                     /gene="secY"
                     /locus_tag="AC162G_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR5074410.1"
                     /translation="MIQAVRNAFVLPDLRRKILFTLLILVIYRAAAHVPVPGINREQL
                     EAVLAGQGAGAPLAQILNLLSGGGLSNFSLLAMGVYPYITASLILQLLTGVVPALQRL
                     QQEEGSEGRRTLEQYTFLLMIPMALLQAIGQIQLFGGTALIDNWGTDTLQMITVLSGM
                     VAGTTFAIWLSNLITEQGIGQGMSIIIFGGIVARLPANIGSLVSDPQGALQSLIAFVF
                     VLVAVIFVIVIFQEGQRRIPVQYGKRVRGRKVYGGGATYIPLRVNMAGMIPLIFAQSI
                     LTLPAILANLFSASPNTSVALLFQNISQIFSGASGHPAAWIYWLMYFLLVVGFTFFYT
                     TVILQQQNLAESLQKQGGFVPGIRPGKRTEEYMNSVTGRITLVGALFLGGVAILPYLL
                     SGITLNGIPLIPNATNFSILSTGLLIVVGVVLDTMRQLEAQLLMRHYEGFIK"
     gene            11783..12499
                     /locus_tag="AC162G_00930"
     CDS             11783..12499
                     /locus_tag="AC162G_00930"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR5074411.1"
                     /translation="MADYILLFGPPGVGKGSQAERLSESLKLPHVATGDLFRDNVKRE
                     TPLGLLAKGYMSRGELVPDEVTVAMLKERLDRPDAVRGALLDGFPRTVAQAAALARLL
                     AERGGSIRVVLFISAPEEVLLTRLASRWTCGRCGAIYNATTSPTREPGVCDRCGGPVE
                     QRADDSPDVQRQRIRVYLEQTLPLVEYYRQRGMLVEIDGRRSIEEVYTDILEAIERSR
                     RDKRELGEFTDGDRPISDNL"
     gene            12496..13245
                     /gene="map"
                     /locus_tag="AC162G_00935"
     CDS             12496..13245
                     /gene="map"
                     /locus_tag="AC162G_00935"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR5074412.1"
                     /translation="MIVLKSAQEIALMRQAGRIVAEVLEKMRALARPGVTTAEIDRAA
                     TELIEQRGGRPTFKGYRGYPANICTMINYELVHGIPSARRRLKEGDILSLDVGVTYKG
                     VVGDAGTTYPIGAISPVARKLLEVTEASLYVGIAQACAGNRAGDISSAIQRYVESHGF
                     TVIREYTGHGVGRQMHEEPQVPNFGKAGRGIVLKPGMTLAIEPMVAQGDWRTRVLDDG
                     WTVVTADGSLSAYFEHTIAVTSGEPEILTKL"
     gene            13311..13424
                     /gene="rpmJ"
                     /locus_tag="AC162G_00940"
     CDS             13311..13424
                     /gene="rpmJ"
                     /locus_tag="AC162G_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR5074413.1"
                     /translation="MKVSASVRKRCPKCKIIRRKGKIRVICSNPKHKQVQG"
     gene            13437..13805
                     /gene="rpsM"
                     /locus_tag="AC162G_00945"
                     /pseudo
     CDS             13437..13805
                     /gene="rpsM"
                     /locus_tag="AC162G_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
     gene            13847..14248
                     /gene="rpsK"
                     /locus_tag="AC162G_00950"
     CDS             13847..14248
                     /gene="rpsK"
                     /locus_tag="AC162G_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR5074414.1"
                     /translation="MAGSQRGRRASAPKKQRRGVSRGQAFIYARFNNTIVSITDPGGN
                     VLSWSSAGQVGFKGSRKSTPYAARLAGQAAARAALDTGMTEVDVFVKGPGPGREQALR
                     ALGASGLRVLSITDVTPIPHNGCRPKKKRRV"
     gene            14281..14931
                     /gene="rpsD"
                     /locus_tag="AC162G_00955"
     CDS             14281..14931
                     /gene="rpsD"
                     /locus_tag="AC162G_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR5074415.1"
                     /translation="MARTIGPVCKLCRREGEKLFLKGARCLSPKCAIERRGYAPGQHG
                     RTAQFKRGRGRSTDYLLQLREKQKARRIYGVMERQFRRYFNIALRSKGLTGVMLLQML
                     ESRLDNVVYRLGLAASRGQARQLVQHGHVDVNGHRLDAPSYLVKMGDVISVRPASRTR
                     SYFKTLAQELEDRQASVPRWLSADPSQLAGRVLALPNREDIDIKLNEQLIVEYYSR"
     gene            15095..16099
                     /locus_tag="AC162G_00960"
     CDS             15095..16099
                     /locus_tag="AC162G_00960"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR5074416.1"
                     /translation="MASLSMVLPKIETDESTQNYGRYVIGPLEQGYGITLGNALRRVL
                     LSSLPGAAVTSMRMSDVYHEFTHIPHVREDMVQVILQVKQLRVKLHGEEPVRLRLEVR
                     GEGTITASDIQCPPEVEIINPDLYLFTVDSDKARLEMELTVEPGRGYSPAEERGRLPI
                     GELPVDAIFSPVRRARFDVERARVGQMTNYDKLVMEIWSDGTMRPQEALSQGARILMQ
                     HFSLVAGVTEEAQMEKVPQAASMIPQEIYDTPIEQLDLSVRVFNSLKRTGITNVGEVL
                     EMLARGEDAMLTIRNFGTKSYLELKEKLVAKGFLKEDEVLPGEVQPSEEPAEAETESN
                     "
     gene            16179..16679
                     /locus_tag="AC162G_00965"
     CDS             16179..16679
                     /locus_tag="AC162G_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013369.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL17 family ribosomal protein"
                     /protein_id="HIR5074417.1"
                     /translation="MRHRVAGKKLNRSSDQRAALRRGLISDLFRHEAIITTQARAAAI
                     RGQAEKLITLARNRGNAGDLIELAKGGDRATLARRTTPTQAEMLLRVAQQKPDELDKA
                     AAGIAASARRQAARVLYGPDVIKRLFDEIAPRYSTRNGGYTRTFKLGMRKGDAAPMVK
                     IELVGE"
     gene            16691..17425
                     /gene="truA"
                     /locus_tag="AC162G_00970"
     CDS             16691..17425
                     /gene="truA"
                     /locus_tag="AC162G_00970"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR5074418.1"
                     /translation="MRIRATIAYDGTAYHGFQRQSAEREPTIQGELERALSRIVARST
                     TALGAGRTDAGVHASGQVVAFDAEWRHALPDLQRALNAVLPEDVVVLDIAQAADGFHP
                     RYQARSREYRYTIYNAAWRDPLCRLYALHVADPIDIPAMREAARHLIGEHDFAAFGQA
                     TSGDSTVRVMHRVEIGGGRPWITVDLEANGFLYRMVRSIVGSLIEVGRGKISVDRFVE
                     ILHSRERGQAETTAAPHGLCLMRVNY"
     gene            17521..17949
                     /gene="rplM"
                     /locus_tag="AC162G_00975"
     CDS             17521..17949
                     /gene="rplM"
                     /locus_tag="AC162G_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR5074419.1"
                     /translation="MKTYNVTKEEIQREWVLVDAEGQTLGRLASRIAAILRGKHKPTF
                     APWLDCGDYVIVVNAGKIKVTGNRMDSKVYYRHSGYVGGLKSVTLRRQLEKHPERVLA
                     AAVRGMLPNNRLGRKMVKKLKLYTGSDHPHAAQQPKKLEL"
     gene            17960..18352
                     /gene="rpsI"
                     /locus_tag="AC162G_00980"
     CDS             17960..18352
                     /gene="rpsI"
                     /locus_tag="AC162G_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR5074420.1"
                     /translation="MAGEYYEGVGRRKTAVARVRLTPGGGGIVVNDKPAEEYFGREAD
                     RHTISSPLIATGTQGAYGISVKVAGGGITGQAAAVRMGVARALLAVDPDFRPVLRQNG
                     FLTRDARAKERKKPGLKRARKAPQYTKR"
     gene            18503..19867
                     /gene="yabN"
                     /locus_tag="AC162G_00985"
     CDS             18503..19867
                     /gene="yabN"
                     /locus_tag="AC162G_00985"
                     /inference="COORDINATES: protein motif:HMM:NF052436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     methyltransferase/pyrophosphohydrolase YabN"
                     /protein_id="HIR5074421.1"
                     /translation="MALITLVGLGPGDLRLLTREAWDVLTGAHEVYLRTVRHPAVSGL
                     PASLRIHSFDAIYEQKDDFAQVYETIAAQVVALGRRPEGVIYAVPGHPRVGEATVSRI
                     LELAGAEGLQVRTVAGLSFVEPALDALGIDALDGLQVADAIEVAARHHPPLDPDRAVL
                     LAQLYSRAVASNVKLTLMNQYPPEHIVKLVHAAGTAEARVVALSLAEMDRRDDVTHLT
                     ALYVPPLPRTSGFSSFQDTIAHLRAPDGCPWDREQTHQSLRPYLLEEAHEVLEAIDAD
                     DMEALREELGDLLLQVVLQTQVATDAEAFRMADVIAGINEKIIRRHPHVFGDVDVAGV
                     DDVKRNWEAIKVAEREESDKPSRESILDGIAKGLPALAQAEMYGARAARVGFDWRDVS
                     GVLDKVAEELREIADARDPETREAEFGDLLFSLVNVARWLEIDPESALRAANARWSMR
                     FREV"
     gene            20049..20633
                     /locus_tag="AC162G_00990"
     CDS             20049..20633
                     /locus_tag="AC162G_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR5074422.1"
                     /translation="MEVIAGVAAIGAVIALVGAAIFFYQTREAVRASRRALFDTERQV
                     YLDRARRGRLLALGFLILTSVLFVVNVGGSAIANPPPTPTPTLTPTPTRPPPTPGPSP
                     TPTPPPTATPTEAPPTPTPPPRTATVTGAGSLGLRLRDAPNGNLIDYLPDGTVVVLLP
                     VESVRTDDGIEWVNVVDPQGREGWMALQYLAINP"
     gene            complement(20623..21915)
                     /gene="aceA"
                     /locus_tag="AC162G_00995"
     CDS             complement(20623..21915)
                     /gene="aceA"
                     /locus_tag="AC162G_00995"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HIR5074423.1"
                     /translation="MHDQEQAIELANDWTTNPRWAGITRAYPASEVVRLRGSIQLHYT
                     LATMGATRLWHLLHTEPFIRALGALTGNQAVQQVAAGLKAIYVSGWQVAGDANDALHM
                     YPDQSLYPVSSVPNLIKRVNNALIRADQIQHMQGKNDVHWFAPIVADAEAGFGGPLNT
                     FELIKAMIEAGAAAVHLEDQLSSLKKCGHMGGKVLVAVHEFVPKLIAARLASDVLGVP
                     TIIIARTDANGAHLIRSINDPVDEKFATGETTVEGYWVVNGGLEYAIAKGLAYAPHVD
                     AIWCETSTPDIGEAREFAQAIHEKFPGKILAYNCSPSFNWKRNLDQKSMATFQEQLGE
                     MGYKFQFVTLAGFHTLNASMFELAHAYKTEGMLAYSRLQEREFEMERTHGYRAIKHQS
                     FVGAGYFDEIQIAVTGEGVSTTAMTGSTEEEQFAVAMD"
     gene            complement(21998..>22274)
                     /locus_tag="AC162G_01000"
     CDS             complement(21998..>22274)
                     /locus_tag="AC162G_01000"
                     /inference="COORDINATES: protein motif:HMM:NF044584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HIR5074424.1"
                     /translation="LMEDTATAEISRAQVWQWINHPTASLPDGRKVAAELVRSMLPEE
                     MEKIRSLYGNELFAAGKFQLAGEIFERLVTAKDFAEFLTLIAYDYLD"
BASE COUNT         4289 a         6579 c         7672 g         3734 t
ORIGIN      
        1 ggccggggtc gatccggccg cacaccacgt ttgacgcgga ggtgtacgtg ctgaagaagg
       61 aagaaggcgg gcggcacaag gcgttcttcc cggggtatcg gccgcagttc tacattcgga
      121 cgatggatgt gacgggcgag attgcgttgc cggccggggt ggagatggtg atgccggggg
      181 acaacgtgaa catgacggtg aagctgatca tgccggtggc cttggaggcg ggcagccact
      241 tcgcgatccg cgaggggggc ttgaccgtcg gcgccggcgt catcaccaag gtgtacgaat
      301 aagcatcgtc tcagacttcc gaagtcttca agacttcgga agtcttcgaa gggatcgagc
      361 gagcctatgg tcaagcagag aatccgcatc cgcctgaaag catacgatca tcgtcttctg
      421 gatcagtccg ccaagcgcat tgtcgaggcg gcggagcgca caggcgcgac caccgtcggt
      481 ccggtgccgc tgcctacacg catcgagcgc ttcacagtgc agcgcggggc cttcatcgac
      541 aagaacagcc aggaacactt cgagattcgc acccataagc gcctgatcga tgtcgttgat
      601 cctgactcga agaccatcga cacgctgatg cgattgaacc tgccggctgg cgtcgacatc
      661 gagatcaagt tttagtcgtg tagtccggta gtcgggtggt cctgtagtcg ggtggttcgg
      721 cagtctggca gctatccgtg tctccgtgac cgaatccgtg tttgagaggg tcgtgatgaa
      781 gggtatcctc ggcagaaaaa tcggaatgac acaaatcttt gctgagaacg gagaacgcgt
      841 accggtcacc gtgatcgagg ctggtccgtg caccgtgacg caggtgcgca ctgtcaaaga
      901 gaacggctac agcgcgattc agattggatt cgatccggct aagaaagcgc agcggctgtc
      961 gcgcggcgag cggggtcatc tcggcctgct caagccggac gagaagcatc ccaaacggag
     1021 gaaattcccc acggatgtgc cgccgctgaa atcgctgcgc gagattcgcg ttcgagagga
     1081 cgagcagtac acggaaggcc agatcatcaa ggccgatatc tttgaggtag gggagcgcgt
     1141 cgacgtcatc ggcacgtcca aaggtcgagg attcgccggc accgtgaagc ggcacggctt
     1201 cagcggcggc tccaagaccc acggccagtc cgaccggatg cgagcgcccg ggtccatcgg
     1261 cgcaggcacg acgcccggca aggtgatcaa aggcaagaaa atggccggcc acatgggcac
     1321 ggatcgcgtg acggcccagc atttggatgt gatcctggtc gatgcggagc gaaatctgat
     1381 cggtgtgcgc gggagcgtgc ccggcgcaaa cggcgactta gtcctggttc gagaaacgcg
     1441 aaagaagtga tgtggtcgag cggccgagtg gtcatgtagt cggttaggtg agcgggtggt
     1501 cagacggccg gctacaggac gattggacta agtgaccact ggactgaccg aggagcggcg
     1561 tgatgaaggt tcccgtcaaa aacatggccg gtgagcatgt cggcgaagtc gagctgaaca
     1621 agctgatttt tgaggcgccc atcaatacgg cgctgatgca tcaggcgctc acgcgccagt
     1681 tggcaaatgc gcggcagggc accgccaaga ccaagacacg cggcgaggtg gccggcagca
     1741 cgcgcaaggt gtggcggcag aagcataccg ggcgcgcgcg ccagggcagc cgcaaggcgc
     1801 cgcagtggcg caagggcggc acggtgttcg gcccgagtcc gcgctcgtac cggcagcgca
     1861 tgccgaagaa catgcggcat gcggcactgc gctcggcgct gtcggcgaag gcggcagacc
     1921 atcagatcgt cgtgctggat gctctgatgc tggatgcgcc caagacgaaa gcgatggccg
     1981 acatgctgcg caacctcgag gccggccgct cggcgttgat tctgttgtcc gagggcagcc
     2041 ccaccgtcga gcgctcgatt cggaacctgg aaggcgtgca gtatctgcgc gcgcaatacc
     2101 tgaacgtgcg ggatttgctg ggtgcggata cggtcgtcgt tccgcaaagc gcgctggctg
     2161 ccatcgacga gctgctcggt tagagtatgc cctgagcgta gtcgaagggt cgaagggacg
     2221 cggcggagga ttgccctcag cgtctcagcg attagcgccg tgcgacggag gaatggtagc
     2281 gatgcaagtc tatgacgtgt tgaagcgccc gctgctcaca gaaaagatga cctatctgcg
     2341 cgatctcaac cagtacgctt tcgaggtcga tgggcgcgcc aacaaagaac agatcaagca
     2401 ggccgtcgaa gcgatcttca aggtcgatgt cgtggcggtg cggatcatgc atcagccggc
     2461 caagcgccgc cgccatctac gcagccgcca gatcggaaag aaggccaagc aggctgtccg
     2521 gcatccgggc tggaagaagg cgattgtcaa gctggcggcc aatcagcgca tcgagctgtt
     2581 cgagggcgtg tagccggtgt gcaagggcgc gtcagcacgc tgaacgcctg cgctcgcgcg
     2641 gcaggcgcgg gcgtgcgaac ccgacgggcc tgccctgagc gtagtcgaag ggtcgaaggg
     2701 gcgccgttca agcgtgaagg cgtgaggaga caccatcgtg gcgatcaaga agtacaaacc
     2761 gacctcgccg gggcgtcgcg gcatgaccgg cgatacattc gaagacatca ccaagacgaa
     2821 gccggagcgc tcgctgacgg tcagcctcaa gaagcacagc ggccggaacg tgcaagggcg
     2881 cgtgacggtg cgtcaccggg gcggcggggc gaaacggcgc tatcgcctga tcgactttcg
     2941 acgcgacaag cacggcatgg cggcgaaagt ggcagccatc gaatatgacc cgggccgctc
     3001 ggcgcgcatt gcgctgctgc actacgcgga tggcgagaag cgctacatta ttgcgccgtc
     3061 cggcatgaaa gtcggcgata cggtgttcag cggcccgggc agcgacgtgc gcgtgggcaa
     3121 ctgccttcct ctgaaggata ttccactcgg cacgctggtg cacaacgtgg agctgtaccc
     3181 gggccagggc gcgcagttcg tccgctctgc tggcacgcac gcgcaagtgc tggcgagaga
     3241 cgccggctac gtcacgttgc gcatgccctc cggggagacg cgcaaagtgc tggattcgtg
     3301 ctttgcggtg atcggccagg tcggcaacct ggatcacgcc aacgtgaagt taggcaaagc
     3361 gggccgtaag cgccacatgg gtattcgacc ggccgtgcgc ggcacggcga tgtcgccacg
     3421 cgaccaccca cacggcgggg gcgaaggccg ctcgccggtc ggcatgcccg gcccgaaaag
     3481 cccctggggc aaacccacgc gcggcgcgcg cacgcgggtc aacaagcgca ccaatcgctt
     3541 gattgttcgc cgtcgcgtga gcaagcgacg ttagagcttg ccctgagccg aagccctgac
     3601 cctgaacgaa gtgaagggga agggcaggcg aagggcggga ggctgaacca tgtcacgatc
     3661 gctgaagaaa ggaccgttca tcgatccgaa gctgctgcgg aaggttgagg cgatgaaccg
     3721 cagcggggat aagaaggtgc tgcgcacgtg gagccgcgcg tcgacgatct tcccgcagat
     3781 ggtcgggcac accatcgccg tgcacgacgg tcgccggcac gttccgatct acattacgga
     3841 aaacatggtg ggccacaagc tgggcgaatt cgcgccgacg cgcacgtatc ggggtcactc
     3901 gacgagagag aagaccaccg aagtgcgcgg ctagaaaggg aagagcgata tggcgtttga
     3961 ggcacgatgt gccacgcgct tcgtcccgtt gtcgccgcgc aaagttcggc tggtggccga
     4021 tcaggtgcgc ggcttgcaag tcgacgatgc gctggcgctg ctgaagttca tgcccatggc
     4081 cgccgccgcg ccggtctcca aggcgatcgc ttcggcggtt gcgaacggcg aagagaacct
     4141 gggtcttgca cgcgacgatt tgttcgtcaa ggagattcgc gccgacgaag ggccgcggcg
     4201 ccagtggcgg cgcttcggtg cgcgtgggcg gttcaagccg atcacgcgcc gttcgtcgca
     4261 tatcacagtc gtgttgagcg agcgttagag cttgcccctt cgcaggctca gggcaagctc
     4321 tgacagagga agggacgagg acgagaatat ggggcgcaaa gttcatccct atgggtttcg
     4381 actgaaagtc gtgcgcgatt ggcaggggcg ctggttcggc aaacgcggcg ggccgtacct
     4441 ggccatgttg agcgaggatt tggcgctgcg caagcgtatc atggcgaaag tcagccgctc
     4501 gggcgtgtcg cgcatcgaga tcgagcgctt ccccaaccag ctcatcatca ccattcatac
     4561 cgcgaagccg ggtatcatca tcggccgcaa aggcgcgcag gtgaaagagc tgcgcgagtc
     4621 gctcgaagcc atgaccggca agaaggtgaa gctggaggtc gaggaggtca ccaagccgga
     4681 tctggaagcc tcgctcgtcg ccgagaacat cgccggccaa ttggagcggc gcatcagcca
     4741 cggtcgcgcg atgaagcgcg cgatccagca ggcgatgcgc gccggagccg agggcatcaa
     4801 gatcaccgtc gcgggccggc tgggtggctc ggaaatgtcg cgcacggaaa cggtgatgga
     4861 gggccgcgtg ccgcgcagca cgctgcgcgc cgatctggac tatgcccgcg cggaggccct
     4921 gaccaccgcc gggcgtatcg gcgtgaaagt gtggatctac agaggtgaga tactgcccga
     4981 ggcgcgggcc gaggacctgg cgacggcgca ctaaccgcac gagtggtctc gaagagggtg
     5041 aaagcgatgt tgatgccaaa acgggtgaaa ttccgcaagc agcagcgcgg ccgcatgaag
     5101 ggcaaggagc ttcgcggcgc cgaagtctcc ttcggcgagt atggtctgca ggcgctcgaa
     5161 ccgtgctgga tgacggcgcg ccagatcgaa gcggcgcggc gcgcggtcgt gcacttcgcc
     5221 aaacggcgcg gcaagctgtg gatccgcgtc ttccccgata agcccgtgac ccagaaggcg
     5281 gcggagaccc gcatgggtaa gggcaagggc gcggtcgacc actgggtggc ggtcgtcaag
     5341 ccgggccggg tcatcttaga agtcggcggc atggattccg gcgtcgcgcg cgaggcgctg
     5401 cgcctggcgg cgcacaagct gccgatcaag acgcaggtca tcacacgccg cgactgggtg
     5461 gctgccgaag agggaggcgc gaaatgaagg tcagcgagat tcgcgccctc cgcacggagg
     5521 agatccagtc gaagatggac gaggcgcaca agtctctctt caatatgcgc ttccaggcgg
     5581 cggtcggcca gttagaggat ttcaatcgca tgcgggcgat acgccgggac atcgcgcgca
     5641 tgaaaacgat tctgcgcgag cgcgagctgg cggcgcaagg gcgctctcct ctcctgtcgt
     5701 ctgcgacggg agaggggccg ggggtgaggg aatgacggag caacaacgca agcgactggt
     5761 tgggcgcgtg accagcgaca agatggacaa gacggtcgtc gtgaccgtac agcgcttgca
     5821 gcgccacccg ctgtacaaga aggtgattcg ccgcgatcgg cgtttcatgg cgcacaacga
     5881 ggggaaccag gcgcgcgagg gcgatctggt gcgcatcgtc gagtcacggc ccctcagccg
     5941 ccacaagcgt tgggtcatcg aagaggtcat cgagaaggcg cgaggataga ggcatggtcc
     6001 agcacgaaac gcgactgaac gtggccgaca ataccggcgc gcgagagatc ctgtgcatgc
     6061 acgtcctggg cggctcgacg cggcgctacg gacacgtcgg cgacgtgatc gtggcggccg
     6121 tcaagcaggc cgcgccgaac ggctccgtga aaaaaggcga agtggtgcgc gccgtgatcg
     6181 tgcgcacggc caaggaatac gcgcgcccgg acggcagtta catccggttc gacgacaacg
     6241 cggccgtgat catcgacccc gagtctctca acccgaaagg gacgcgcatt ttcggcccag
     6301 tggcccgcga gctgcgcgac aagggcttca tgaagatcgt gtcgctcgcg ccagaagtgc
     6361 tgtaggaggc atctccagat gccgactgtc ggcgcctgac agggagaacg aacatggcca
     6421 ggattaagaa gggtgacacc gtcatcgtca tcgccggcga gaataagggc acgcaaggca
     6481 ccgtgcagcg cgtggacgcc aagacccagc gggtggtcgt gaccggcgtg aacatcgtca
     6541 agaaagctca gcgccctgtg cgcgccggac gcggccagac gcagacgggc atcatcgaat
     6601 tcgaggcgcc catccacatc tcgaacgtga tgctggtcga tccgaaatcc agcgaaccga
     6661 cgcgcgttgg ccacacagtc gacgccagcg gcgccaagcg gcgcgtcacg cgtaagagcg
     6721 gaacggagat ttagaaatca aatggcgaat ctgaagaagc gatatcgaga agaaatcgtc
     6781 ccggcgctga tgagggactt cggctacaag aacgttatgc aggcgccgcg cattcaaaaa
     6841 gtgatcgtca acatcggggc gggcgatgcc ctggagaatg ccaagacgct ggacggcgcg
     6901 gtggccgacc tgcggctgat caccggccag cagccggtga tcaccaaggc gcgcaaggat
     6961 atcgccaact tcaagctgcg cgccgggcgg tcgatcggcg tgaaggtgac gctgcgcggg
     7021 gagcgcatgt ggtcgtttct ggatgtgctc gccaatctgg cgctgccgcg cattcgcgac
     7081 tttcgaggta tttcgccgga cgctttcgac gggcgcggca attatacgct cggcttgcgc
     7141 gagcaaatca tgtttcccca gattcagtac gacaagatcg acaagctgcg cgggatggaa
     7201 attacgatcg tgaccacagc gcgctccgac gaagagggac gccggctgct acatcttatc
     7261 gggatgccgt tcaggagcaa ttgattatgg ccaagaagtc gatgatcaat cgagagtctc
     7321 ggcgcaaata tccgacgcgc gtgcggaacc gctgccgcca gtgcggccgg ccgcgcggct
     7381 acatccggcg cttcgggctg tgccggattt gcttccgcga gctggcgctg cggggagtga
     7441 ttcctggcgt ggctaaagca tcctggtagg ccagcagcca agccctgagc ggagccgaag
     7501 ggcctgccta gagcgaagtc gaaggggcag tcgacgaata cgctcgctcc ggggatgcgg
     7561 gggagcgggg ttcagaggag caatcggcta tgaatataag tgatccgatc agcgacatgt
     7621 tgactcgcgt gcgcaatgct attgccgtga ggcacgccag cgtggcgttg ccgagcagcc
     7681 agatcaagat cgccattgcg accattctga gagaagaagg ttacatccag gatttcgagg
     7741 tgaccgaaag cggcaagcgc aagacgcttc gtctgtggct gaagtacacc ggcgagcggc
     7801 gcgatcggca gccggcgatc tccggcctgc agcgcgtgag ccgacccggt cgacgtgtct
     7861 ataccagcag ggacagcatc ccttgggtgc tctcgggcat gggtatcgcc atcctgtcga
     7921 cgcctaaggg cgtgattacg gggcagcagg cgcgtcgatt gaacgtcggc ggcgaggtgc
     7981 tgtgctacgt gtggtaaacg cgggagctga ggagtctatc atgtcgagaa ttggacgcaa
     8041 gcccgtgccg attccgtcgg gagtcgccgt gaccatcgac ggaccacgcg tcacggtcaa
     8101 aggcccgcgc ggcgagttga cgcgggcatt ccatccggac atggctatcg cgatggatga
     8161 tggcacgctg gtcgtcagcc gtccgagcga tgcgcgccag catcgtgcgc tgcacggcct
     8221 gacgcgcgca ttgttggcca acatggtgac cggcgtgacg caaggattcc gcaagacgct
     8281 cgtcgtcgaa ggcgtaggct accgcgccga cttgaccgga aaagtgttgt tgttgcacct
     8341 gggttactcg catccgatcc gggtcgagcc gccggcgggc gtcaagttca gcgccgattc
     8401 caaggcgcgc acgatcactg tcgagggcgc cgatcgcgag caggtgggac agatcgcggc
     8461 cctgattcgc aaactgcggc cgccggagcc gtacaagggc aagggcattc gctaccaggg
     8521 cgaagtgatt cgccgcaagg ccggcaaggc cggtaaggtc ggttagggct gaggggtgaa
     8581 cgatgccgaa aagtcgcaat gagatgcggt tgcgccgaca gaagcgcgtc cggcgaggcg
     8641 tgaatgggac agccggccgg cctcgcctga acgtgttccg cagcctggag cacatctacg
     8701 ctcaggtcat cgacgacgaa cagggacgga cagtcgtgtc ggcctcgacc attgacaggg
     8761 aactgcgcgc ggaaatagct tcgctgagca agacggagca agccaggcgg gtcggcgccg
     8821 cgttggcccg gcgcgcgcag gccgccggcg tgaagcaggt ggtcttcgat cgaggcggct
     8881 atcgttatca cggccgcgtc aaggcgctgg ccgatgccag ccgagaggtg ggattggagt
     8941 tctgatggca aagcgaagag tctccaccga aagacgtgat cgcgaggtgt tcgaggagcc
     9001 gcgcgaggag ctcgacgagc gcgtggtcga cattgcgcgc gtggccaaag tgatcaaagg
     9061 cggtcgccgg tttgcgtttc gcgtcgtcgt cgtcgcgggc gacaatcgcg ggaacgtcgg
     9121 cgtcggcgtc ggcaaaagcc gagccgtgcc ggacgctatc cgcaaggcgt cggagcgcgc
     9181 gcgcagtgcg atgcgcccgg tcgtgctgga aggcacgacg attccgcatc cggtgatggc
     9241 caagtcggga ggagcgaggg tgatgctgcg cccggcatcg ccaggcacgg gcgtgatcgc
     9301 gggcggcggt gtgcgcgccg ttctcgaagc cgccggcgtg cacgacatcc tgaccaagtc
     9361 gatggggagc agcaacaagt tgaacgtgat acgcgccacg ctggccggcc tgcgccagct
     9421 caagtcgccg gatgccgtgg cgcaggagcg cgggaagccg attacggatg tggcgccgtt
     9481 ttggagaagg actcatgtca agcagccgta agcctgccct gagcgaagcc cgccctgagc
     9541 gtagtcgaag gggcgaaggg tccaccggcc agctgcttga cgcagatcct cgtagcggag
     9601 cggaggggac gccccgccca gcgaggttgc gcatcactct ggtgaaaagc ccgaccggct
     9661 acgacgagca ccaaaagcgc acggtgcggg cgcttggcct gcgtcgcctt cgccagaccg
     9721 tcgagcaggc ggatacgccg gtcgtgcgcg gcatgattgc caagatcccg cacctggtat
     9781 ccgtcgagcc tgccccgagc gaagcccgcc ctgagcgcag tcgaagggtc gaaggggaag
     9841 tgaaatgaaa ttgcacgaac tgaaaccggt agaaggttcg aaaaagcggc gtaagcgggt
     9901 agggcgcggc ctcgggtccg gttccggcac gtacgcgggg cgcgggcgca agggccagca
     9961 tgcaagacag ggcggcgtga agggtccgta tttcgagggc gggaaccttc cgctcgtgcg
    10021 caagctgccg ttcgcgcgcg gcgtcggctt cacttcgcgt gggaagatcc actatgtccc
    10081 gatcaacctg tccgacctgg ccgcgctcgc cagccgaagc cctgagtgga gcgaggggga
    10141 gccggtgacg ccggaggtgc tgcaccgcca cggcttgctc aagtcggccg acgatccggt
    10201 ggtgattctc gcctacggcg agatcgagcg cgccgtgacg gtgagggcgc atcgtttttc
    10261 cgcgtctgct cgccgcaaaa tcgaggcggc cggcggcacg gttgagatgc tgtagaggcc
    10321 actatgattc aagcggtccg aaatgccttc gtgttgccgg atctgcggcg caagatcctt
    10381 tttacgctgc tgatcctggt catctaccgc gccgcggctc acgttccggt gcccggtatc
    10441 aatcgggagc agttggaggc ggtgctggct ggccagggcg ccggcgcgcc gctggcccaa
    10501 atcctgaact tgctgtccgg cggcgggttg tcgaatttct cgctgctggc gatgggcgtg
    10561 tacccgtaca tcacggcgtc gctcatcttg cagttgctga ccggcgtggt gcccgctctg
    10621 cagcggctcc agcaggagga gggatcggaa gggcgacgca cgctcgagca atatacgttt
    10681 ctcttgatga tcccgatggc gctgctgcag gcgatcgggc agattcaatt gttcggcggc
    10741 accgccctga ttgacaactg gggcacggac acactgcaga tgatcacggt tctgtcggga
    10801 atggtcgccg gcacgacgtt cgcgatctgg ctcagcaact tgatcaccga gcaaggcatc
    10861 ggccagggca tgtcgatcat catcttcggc ggcattgtgg cgcggctgcc tgccaacatc
    10921 gggagcctgg tgagcgatcc gcagggcgcg ctgcagtccc tgattgcctt cgtcttcgtc
    10981 ctggttgcgg tgatcttcgt catcgtcatc ttccaggaag gccagcggcg catcccggtg
    11041 cagtacggca agcgtgtgcg gggccgcaag gtctacggcg gcggcgcgac ttacattccg
    11101 ctgcgcgtca acatggccgg catgattccg ctgatcttcg cgcagtccat cctgacgctg
    11161 ccggcgattc tggccaacct gttttccgcg tcgccgaaca cgtcggtggc gctgttgttc
    11221 cagaatatca gccagatctt cagcggcgca tcaggccatc cggcggcatg gatatactgg
    11281 ttgatgtatt tcctgctggt cgtgggattc acgttctttt acacgaccgt catcttgcag
    11341 cagcagaacc ttgccgaatc gctgcagaaa cagggcggat tcgtcccggg tatccggccc
    11401 ggcaagcgca cggaagagta catgaattcg gtgacgggcc gcatcacgct ggtcggcgcg
    11461 ctgttcctgg gtggggtagc gatcttgcct tacttgctca gcgggatcac gctcaacggt
    11521 attccgctga ttccgaacgc gacgaacttc tcgatcttga gcacgggcct gctgatcgtc
    11581 gtcggcgtgg tgctggacac gatgcgccag ttggaggcgc agttgctgat gcgccattat
    11641 gaaggcttta ttaagtaatc agtaattagt aatcagtgac cagtcatcag tgaccggtgt
    11701 gttgagataa cccatcatgt gctaactgac gaccggtcat tgatgactga gcagagcggc
    11761 agagaaaggc tggggcgaga tcatggccga ctacatcttg ttgttcggcc cgcccggcgt
    11821 cggcaagggc agccaggccg agcggctcag cgaaagcctg aagctgccgc acgtcgcaac
    11881 gggcgatctg ttccgcgaca atgtgaagcg ggaaactccg ctcggtttgt tggcgaaggg
    11941 ttacatgtcg cgcggcgagc tggtgcccga cgaagtcacg gtcgcgatgc tgaaggagcg
    12001 gctcgatcgg cctgacgccg taagaggcgc gctgctcgac ggcttcccgc gcacggtggc
    12061 acaagcggcc gcgctggccc ggttgctggc cgaacgcggt gggagcattc gtgtcgtgct
    12121 cttcatctcg gctccggaag aagtgttgct cacgcggctc gccagccgat ggacgtgcgg
    12181 gcggtgcggc gcgatttaca acgcgaccac cagcccgacg cgcgagcctg gtgtatgcga
    12241 tcgatgcggc ggcccggtgg agcagcgggc ggacgacagc ccggatgtgc aacgccagcg
    12301 cattcgggtc tacctcgagc agactttgcc gctggtggag tattatcggc agcggggaat
    12361 gctggtcgaa atcgatgggc gccgatcgat agaggaagtt tacacggaca ttttggaggc
    12421 catcgaacgg tcgcgccgtg ataagcgcga attgggtgaa ttcacggatg gcgatcgtcc
    12481 gatcagcgac aacttatgat cgttcttaag tcggcgcagg agattgcatt gatgcgccag
    12541 gctgggcgca tcgtggccga ggtcctggag aagatgcgcg cgctggcgcg gccgggcgtg
    12601 acgacggctg agatcgaccg ggcggcgacg gagctgatcg agcagcgcgg cggacgcccg
    12661 accttcaaag gctatcgggg ctatccggcg aacatctgca cgatgatcaa ctacgagctc
    12721 gtgcacggta tcccgagtgc tcggcgccgc ctgaaggaag gcgacatcct gagcctggac
    12781 gtgggcgtga cctacaaggg agtcgtcggc gacgccggca cgacgtatcc tatcggcgcg
    12841 attagcccgg tcgccagaaa gctgctggag gtgacagagg cgagcttgta cgtcgggatc
    12901 gcgcaggcgt gcgccggcaa ccgcgcgggc gacatttcgt ccgccattca gcggtatgtc
    12961 gagtctcacg gctttactgt gatccgcgag tataccggcc atggggtcgg gcggcagatg
    13021 cacgaggagc cgcaggtgcc caatttcggc aaggccgggc gcggcatcgt gttgaagccg
    13081 ggcatgacgc ttgccatcga gccaatggtc gcgcagggtg actggcgcac gagggtgctg
    13141 gacgatggct ggacggtggt gactgccgac gggtcgctat cggcctactt tgaacatacg
    13201 atcgccgtca ccagcggcga accggagata ttgacgaagt tatagtaagt ttcagacttc
    13261 cgaagtcttg gagacttcgg aagtctggag cgatagagga gtgttctagc atgaaagtct
    13321 ctgcatccgt tcgaaagcgc tgcccgaaat gcaagatcat ccgccgcaag ggcaagattc
    13381 gcgtgatctg ctcgaatccc aagcacaagc aggttcaagg ctaggcagga ggcaacatgg
    13441 cacgcatcgc aggggtcgat ttgccgcgca acaagcgagt ggcagtcggg ctgacctcca
    13501 tctacggcat tggtccatcg aacagcctcg acatactgca tcaggctcag gtgagtcctg
    13561 acatgcgcat caaggatctg tcggaagcgg aagtatccag gctgcgcgag ctgatcgagc
    13621 agaactacgt cgtggagggc gatctacggc gcgaagtgac gctgaacatc aagcggctca
    13681 cggagatcgg ctgctatcgc gggatccgcc atcggcgcgg cttgccggtg cgcggccagc
    13741 gcacccgcac caacgcgcgc acgcgcaaag gccctaaaaa gacggtggcc ggccgcggcc
    13801 ggcgtcgcgg cgcaagaaga agtaaaagac ataggagaga gaagcgatgg caggcagtca
    13861 acgaggccgg cgcgcgagcg cgccgaagaa gcagcgccgc ggtgtgtcac gcggccaggc
    13921 gttcatctac gcgcggttca acaacactat cgtgtcgatc accgatccgg gcggaaatgt
    13981 attgagctgg agctcggccg ggcaggtcgg attcaagggc agccgcaaga gcacgcccta
    14041 cgccgcgcgc ctggccgggc aggccgccgc ccgggccgcg ctcgacacag gcatgaccga
    14101 agtggacgtg ttcgtgaaag ggccggggcc gggccgcgag caggccctgc gtgcactggg
    14161 cgcgtccgga ctgcgcgtcc tatcgattac ggatgtcacg ccgatcccgc acaacggctg
    14221 ccgtccgaag aagaagcgtc gcgtttagtc acatagtcgt ttagtcatac aggaggaagc
    14281 atggccagga cgattgggcc tgtgtgtaaa ctgtgccgcc gcgaaggcga aaaactgttc
    14341 ctcaaaggcg cacgctgttt atcgcccaag tgtgccattg agcggcgcgg ttacgccccg
    14401 gggcagcacg gcaggaccgc tcaattcaag cgcgggcgcg ggcgctcgac ggattatctc
    14461 ttgcaactgc gcgagaagca gaaggcgcgc cgcatctacg gcgtgatgga gcggcagttc
    14521 cgccgttatt tcaacattgc gctgcgcagc aaagggttga caggcgtcat gttgctacaa
    14581 atgctggaaa gccgcctgga caacgtcgtg taccgcctgg ggctggccgc gtcgcgcggg
    14641 caagcgcgcc agttggtgca gcacggccac gtcgacgtga acgggcaccg cctcgacgcg
    14701 ccgtcctatc tggtcaagat gggcgacgtg atttcggtgc ggcccgccag ccgcacgcgc
    14761 agctatttca agacgctggc gcaggagctg gaagatcgcc aggcgagcgt gccgcgctgg
    14821 ttgagcgcgg acccttccca gctcgcgggc cgggtgctcg cgctgccgaa tcgtgaagat
    14881 attgacatca agctcaacga gcagctcatc gtcgaatact actcgcgcta acatcgctgc
    14941 ttacgccccg cccagcgggg tcgtgaagaa gtcgggcaac tggccgatat ccgcatcgcg
    15001 ctccgagcgc tgtgtgatat cgctagacag gcaacctttg gttgccgctt gccggtacga
    15061 ctgatgggca ggcggtcgaa atggaggcct tacattggct agtttaagca tggtcttgcc
    15121 gaagatcgag acggacgaat cgacccagaa ctacggccgg tacgtcatcg gaccgctcga
    15181 gcaagggtat ggcatcaccc tgggcaacgc gctgcgccgc gtgctgctca gcagcttgcc
    15241 gggcgcagcc gtcacgtcga tgcgcatgag cgatgtgtat cacgagttca cgcacatccc
    15301 gcacgtgcgc gaagacatgg tacaggtgat cctgcaagtc aagcaattgc gcgtcaagct
    15361 gcacggcgag gaaccggtgc gactgcgcct cgaagtgcgc ggcgaaggga ccatcaccgc
    15421 cagcgacatc caatgcccgc cggaagtcga gatcatcaat cccgacctgt atttgttcac
    15481 cgtcgacagc gacaaggcgc ggctggagat ggaattgacc gtcgagcccg gccgcggcta
    15541 cagtccggcc gaggagcgtg gacgcctgcc catcggcgag cttcccgtcg acgccatctt
    15601 cagcccggtg cgccgcgctc gcttcgacgt agagcgcgcc cgcgtcggcc agatgaccaa
    15661 ctacgacaag ctggtgatgg agatttggag cgacggcacg atgcgcccgc aggaggcctt
    15721 gtctcagggg gcgcgcattc tgatgcagca cttctcgctg gtcgccggcg taaccgaaga
    15781 ggcgcagatg gaaaaagtgc cgcaagcggc ctcgatgatc cctcaggaga tttacgacac
    15841 gcccatcgag cagcttgacc tgtcggtgcg cgtcttcaac tcgctcaagc gcacgggcat
    15901 cacgaacgtc ggcgaggtcc tggaaatgct cgcccgcggc gaggatgcga tgctcacgat
    15961 acgcaacttc ggcaccaagt cgtatctgga gttgaaggaa aaactcgtcg ccaaaggctt
    16021 cctcaaggaa gacgaagtgc tgccgggcga ggttcaaccg tccgaagaac ccgccgaggc
    16081 agagacggaa tcgaactgac acgggcgccc tgagcggagt caaggcttta gccgggacga
    16141 agccatcgtt ttcagcgtct cggcgatagg gtgagggtat gcgacatcga gtagcaggca
    16201 agaaactgaa tcgctcatcg gatcagcgcg cggcgctgcg gcgcggcctg atcagcgacc
    16261 tgttccgcca cgaggcgatc atcacgacgc aggcgcgggc ggctgccatt cgcgggcagg
    16321 ccgaaaagct gatcacgctg gcgcgcaacc gaggcaatgc cggcgatctg atcgagctgg
    16381 cgaagggcgg cgatcgcgca acgctcgcgc ggcgcacgac gccgacgcag gccgagatgc
    16441 tcctgcgcgt cgcacagcag aaaccggacg agctcgacaa agcggccgcc ggcatcgccg
    16501 ccagcgcgcg gcggcaagcg gctcgcgtgc tgtacggccc ggacgtgatc aagcgattgt
    16561 tcgacgagat cgcgccgcgc tacagcacgc gtaacggcgg ctacacgcgc accttcaagc
    16621 tgggcatgcg caaaggggac gccgcgccga tggtgaaaat cgagctggtg ggcgagtaag
    16681 gtggtgtggg gtgcgcatcc gcgccacgat cgcgtacgac ggcacggcgt atcacggttt
    16741 tcagcggcag tcggcggagc gtgaaccgac gattcagggc gagttggagc gcgcgctttc
    16801 ccggatcgta gcgcgctcga cgacggcgct gggagcgggg cgcaccgacg ccggcgtgca
    16861 cgcgagcggg caggtcgtcg ctttcgacgc cgaatggcgg catgccctgc cagacctgca
    16921 acgggcgctc aacgcggtgc tgcccgaaga cgttgtcgtg ctggacatcg cgcaggccgc
    16981 cgatgggttc catccgcgct atcaggcgcg cagccgcgaa tatcgttaca cgatctacaa
    17041 cgcggcatgg cgcgacccgc tgtgccggct gtacgccctg cacgtggccg atccgatcga
    17101 tattcctgct atgcgggaag cggcgcggca tttgatcgga gagcatgact ttgccgcatt
    17161 cggacaggcg acgtcgggcg attcgacagt gcgcgtcatg catcgagtcg agatcggcgg
    17221 cggccggccg tggatcacgg tcgatctgga agcgaacgga tttctgtacc gcatggttcg
    17281 cagcatcgtc ggctcgctga tcgaagtcgg gcgcggaaag atctcggtcg accggttcgt
    17341 cgagattctc cactcgcgag agcgggggca agctgaaaca actgccgcac cgcacggtct
    17401 gtgcctgatg cgggtgaact actgaggccg gcgggacaga cctgccctga cccgaagccc
    17461 tgagcggagt caaggcttta gccggagggt cggcgagcac cgcatggcag aggagtcaac
    17521 gtgaagacgt acaatgtgac gaaagaggag attcaaagag agtgggtcct ggtcgatgcg
    17581 gagggacaga cgctgggccg cctcgcgtcg cgcatcgcgg ccatcctgcg cggtaagcac
    17641 aagccgacct tcgcgccgtg gctcgattgc ggcgactacg tcatcgtcgt caacgccggc
    17701 aagatcaagg tgaccggcaa ccgcatggac agcaaggtct actatcgcca ctccggctat
    17761 gtgggcggct tgaagtcggt gacgctgcgc cgccaactgg agaaacaccc ggagcgtgtg
    17821 ctggcggcgg cggtgagggg catgctgcct aacaatcgcc tgggtcgcaa gatggtcaag
    17881 aagctcaagc tgtacacggg cagcgaccat ccgcatgctg cgcagcaacc taagaagctg
    17941 gaactgtagg gagaggatta tggcaggcga gtattacgag ggtgtgggcc ggcgcaagac
    18001 ggcggtcgcg cgcgtgcgcc tgacgccggg cggcggcggc atcgtcgtca acgataagcc
    18061 ggctgaagaa tatttcgggc gcgaggccga tcgccacacg atcagcagcc cgctgatcgc
    18121 cacgggcacg cagggcgcct acggcatcag cgtgaaggtc gcaggcggcg gcattaccgg
    18181 acaggcggcc gccgtgcgga tgggggttgc gcgtgcactg ctggccgtcg atcctgattt
    18241 tcggcctgtg ctgcgccaga acgggttcct cacccgcgac gcgcgcgcta aagagcgcaa
    18301 gaagcccggc ctcaagcgcg ctcgcaaagc gcctcagtac acgaagcgct gagtcgttct
    18361 gaggcgcatc cgtttgcgcc gaagaatctc agcacgcggc cgcctgaacc acccggagtc
    18421 aaggctttag ccggattcgc ctggcataag ccggctgccg tggggcgggc agcgcgcctg
    18481 ccccctcagc gcctcagcga tcatggcatt gattaccctc gtcggcctcg gtcccggcga
    18541 tctccgcctg ctcacccgcg aagcgtggga tgtgctaacc ggcgcacatg aggtatactt
    18601 gcgcacggtt cgccatccgg cggtctcggg cctgccggcg tcgctgcgca ttcattcgtt
    18661 cgacgcgatt tacgagcaga aggacgactt cgctcaggtc tacgagacta tcgcagcgca
    18721 ggtggtggcg ctgggccgca ggccggaggg cgttatttac gccgtgccgg gacatccccg
    18781 agtcggcgag gcgaccgtca gccggattct ggaactggct ggggccgaag ggcttcaggt
    18841 tcggacggtc gccgggcttt ccttcgtcga gccggcgtta gacgccctgg gcatcgacgc
    18901 cctggatgga ttgcaggtgg ccgacgcgat cgaggtcgcg gcgcggcatc atccgccgct
    18961 cgacccggat cgagccgtcc tgctcgccca actgtactcg cgcgcggtgg cctcgaacgt
    19021 gaagctcacg ctgatgaacc agtatccccc agagcacatc gtgaagctgg ttcacgctgc
    19081 cggcacggcc gaggcgcgcg tcgtggcact gtcactggcc gagatggatc ggcgcgacga
    19141 cgttacccat ctgacggcgc tgtacgttcc gcctctgccg cgcacaagcg ggttcagcag
    19201 ctttcaggat acgatcgctc acctgcgcgc gccggacggc tgcccgtggg atcgcgagca
    19261 gacgcaccag tcgctccggc cgtacctgtt ggaggaggcg cacgaggtgc tggaggcgat
    19321 cgacgccgac gacatggagg cgctccgcga ggagctgggc gatctcctgc tgcaggtcgt
    19381 gcttcagacc caggttgcaa cggatgcaga agcgtttcgc atggcggacg tcatcgcggg
    19441 catcaacgaa aagatcatcc gccggcaccc gcacgtcttc ggcgacgtcg acgtggcggg
    19501 cgtggacgac gtgaagcgca actgggaagc gatcaaagtt gcggagcgcg aggaatcgga
    19561 caaaccttcc cgcgaatcga ttctggacgg catcgccaag gggctgccag cgctggcgca
    19621 ggcggagatg tacggcgcgc gcgcggcgcg cgtcggattt gactggcgag acgtttcggg
    19681 tgtgttggac aaggtggccg aggaactgcg tgaaatcgca gacgctcgtg atccggagac
    19741 gcgcgaggcc gaatttggcg acctgttgtt ttcgctggtg aacgtggcgc gctggctgga
    19801 gatcgatccg gaatcggcgc tgcgcgcggc gaatgcgcgc tggtcgatgc gatttcgcga
    19861 agtctagcgc gcggcgcggg cgcagggtaa ggtggtcagc gagctatcgc tcgacgaaat
    19921 ggagcgtttg tggcaggcgg cgaaggctgc cctgagcgga gtcaaggctt tggccggagg
    19981 ggcaaaggag cgcgaggcct gatgggcaac cacaagggtc gcccgtacat catggagggg
    20041 cgaatcccgt ggaagtgatc gctggtgtgg ctgccatagg cgccgtgatc gccttggtcg
    20101 gcgctgccat cttcttttat cagactcgcg aagccgtgcg cgcctcgcgg cgggcgctgt
    20161 ttgacaccga gcgccaggtt tacctggatc gcgcccggcg cgggcggctg ctcgcgctag
    20221 ggtttctgat cctgacctcc gtcttgttcg tcgtcaacgt gggtggcagc gccatcgcca
    20281 atccgccgcc gacgcctacg ccgacgttga cgcctacgcc gacgcggccg cctccgacgc
    20341 caggcccgtc tccaacgccg acgcctcccc cgacggcgac gccgaccgaa gcgccgccga
    20401 cgccgacccc gccgccgcgc acggcgacgg tgaccggcgc aggctctctc ggactccgcc
    20461 tgcgagacgc ccccaacggc aacctgatcg actatttgcc ggatggcacc gtcgtggtgt
    20521 tgctaccggt agagtctgtt cgaaccgacg acggcatcga gtgggtgaac gtcgtcgatc
    20581 cgcagggccg ggaaggctgg atggcgctcc agtacctggc gatcaatcca tagcgactgc
    20641 aaactgctct tcctcggtcg agcctgtcat cgccgtcgtc gacacgccct cgccggtgac
    20701 ggcgatctgg atctcgtcga agtagcccgc gccgacgaag ctctggtgct tgatcgcgcg
    20761 gtagccgtgc gtgcgctcca tctcgaactc gcgttcttgc aggcgcgagt acgcgagcat
    20821 gccctcggtc ttgtacgcat gcgccagctc gaacatactg gcgttcaagg tgtgaaagcc
    20881 ggccagcgtg acgaactgga acttgtagcc catctcgccg agctgctcct ggaacgtcgc
    20941 catcgacttc tggtcgaggt tgcgcttcca gttgaacgac ggggagcagt tgtaggccag
    21001 tatcttgccc ggaaactttt cgtgaatggc ctgggcgaac tcgcgcgcct ctccgatgtc
    21061 tggcgttgac gtctcgcacc aaatcgcgtc gacatgcggc gcgtaggcca gacccttggc
    21121 gatggcgtac tccaggccgc cgttcaccac ccagtatcct tccaccgtcg tctcgccggt
    21181 cgcgaatttc tcgtcgaccg gatcgttgat gctgcgaatg aggtgtgcgc cgttggcatc
    21241 cgtgcgggcg atgatgatcg tcggcacgcc cagcacgtcg gacgcgagcc gcgccgcgat
    21301 gagcttaggg acgaactcgt ggacggcgac gagaaccttg ccgcccatgt gcccgcactt
    21361 tttcagcgac gacaactgat cttccaggtg cactgccgct gcgccggctt cgatcatggc
    21421 cttaatcagc tcgaaggtat tgagcgggcc gccgaagccg gcctcggcgt ccgcgacgat
    21481 cggcgcgaac cagtggacgt cgtttttgcc ctgcatgtgc tgaatctgat cggcccggat
    21541 cagggcgttg ttgacgcgct tgatgaggtt cggcacactg ctcacggggt acaggctttg
    21601 gtcgggatac atgtgcagcg cgtcgttagc gtcgccggcg acttgccagc cgctgacgta
    21661 aatggctttc agcccggccg ccacctgctg cacagcctgg ttgccggtca gcgccccgag
    21721 cgcgcggatg aacggctcgg tgtgcagcaa gtgccacagg cgcgtcgcgc ccatcgtggc
    21781 aagcgtgtag tgcaactgga tcgacccgcg caggcgcacc acttccgagg ccggataggc
    21841 tcgggtgatg cccgcccaac gcggattcgt cgtccagtcg ttggcgagct cgatggcttg
    21901 ttcctggtcg tgcataggaa gtctccttgg gtatgattcg cagacctgcc aggtttcgca
    21961 gacctgccag gtttcgcaag cctggcaggt cttacaatca atccaggtag tcgtacgcga
    22021 ttaaggtcag gaactcggcg aagtctttcg cggtgacgag gcgctcgaag atctcgccgg
    22081 ccaactgaaa cttgccggcg gcgaatagct cgttgccata aagcgatctg atcttctcca
    22141 tttcctccgg cagcatgctg cgcaccaact cggcagccac cttacggccg tccgggagcg
    22201 acgcagttgg atggttaatc cactgccaca cttgtgcgcg cgagatttcg gcggtggccg
    22261 tgtcttccat gagg
//
LOCUS       DBNHXB010000024         5911 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1179987, whole genome shotgun sequence.
ACCESSION   DBNHXB010000024 DBNHXB010000000
VERSION     DBNHXB010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1179987"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2137)
                     /locus_tag="AC162G_01005"
     CDS             complement(<1..2137)
                     /locus_tag="AC162G_01005"
                     /inference="COORDINATES: protein motif:HMM:NF021635.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HIR5074425.1"
                     /translation="MPEWLPSASKKVLAPVDASLLAVLLIALIAALPLVTRPGLPRHT
                     DLELHVFRAAEFERLLAERPLEYPRWSPNFYYGYGYPIFNYYAPLTYYAVGAIALVPV
                     LDIVDAMKIVLVLTFVSAACGAFFFARRHFGGASGVVAAAAYVLSPYVLFVDPFMRGD
                     AAEFLALGILPWVFLAFDRPLSSARDIARAALALAALVLSHNLMALIGAGLLAAWLAW
                     RGVFVDGAGRWLRDVAAVALAAGLTAVFWLPFFAERSAVRLDVAGPGHFDFRNHFVEL
                     GTLLRPSPVLDLGATAPHFIYNLGFAQWLLALPAPLAILRRRSQPAARIAAFFVLASL
                     ALIFLMMPASQAWWEAVPPAALIQFPWRFLGLAAFALAMCAGCTVSTFRRAEIAAAVA
                     LSLVLLTALPAMYPPSWDAEFGDTSPRGAVEFELSGIALGTTSTGDFLPRTFAETPPP
                     AQPLIDAYRTGGAVDRFDYASAAGARVRRSLVRIGDLDAEYDVESSDDFTARFFIFAF
                     PGWRAYVDGKPVPLRPSGGDGFILFDVPASAHTFGVRFESTAPRTAGAIVTLGSLLVA
                     AAIVAVSRLRKSPASADLMAGPSVQRSPRSAIRLLVPLLAAFLAVKVLIVDRCDTCFR
                     YTSPPGQALAAQHAQRASFGGHVELLGFDLPPGDVEAGQVAPLTLYWRATAPVPANYQ
                     VFAHVTRPPFVLWGQSDKLNPGDFPSTRWP"
     gene            complement(2366..3553)
                     /locus_tag="AC162G_01010"
     CDS             complement(2366..3553)
                     /locus_tag="AC162G_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074426.1"
                     /translation="MIRRTFTALASLGLAFVALLVTMAAATRLPPAAALAIVPSHWTV
                     QTVDSPNAGPGASMRLDSQGRPHVSYFDQGLGVLKYAVLSGTQWMTEVVDPRHQTGAE
                     TSLVLDASDRPAVAYLGNVNLNVRYAARISPTWVFSDVDQTGSGPGGQGFSRPALAFD
                     GQGRPHIVYFNFTTLRLMHAYREGGTWLKEAIARVGNLSMLVAAPSVVISGQAPHLAF
                     LSESPDPLSSTQVVTRLYYATLSGTQWVTAVVADSPEAAYTAIALDAQGRPSIAYNEP
                     GAGLRYAVLSGTVWLSETIDRIGDPGEYVSLAFDTDGIPHVGYLADTVGGKARYAYKL
                     GPTWLVQTVTTARPVRGYTAVAVDAAGDPHMAYYTLSVTRTLEYAYVKTRHIYLPVVL
                     KSQ"
     gene            complement(3609..4475)
                     /gene="sucD"
                     /locus_tag="AC162G_01015"
     CDS             complement(3609..4475)
                     /gene="sucD"
                     /locus_tag="AC162G_01015"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR5074427.1"
                     /translation="MSILVDKNTRLIVQGITGREGEFHTRKMVEYGTNVVGGVRFGKG
                     GEWVAGVPVFDTCKEAVDATGANASVIFVPARSAPDAMLEAADAGIPLVVCITEGIPV
                     LDMVRVRAYLDRRGTRLIGPNCPGLITPGVAKVGIIPGQICASGNIGIVSRSGTLTYE
                     VIAALTNRGMGQSTCVGIGGDPIYGTSFLDVLEMFEEDPLTDHVVLIGEIGGNDEERA
                     AEFISARMTKPVTAFVAGRTAPPGKRMGHAGAIIEGGTGTAAEKIAAFERHGVRVAQH
                     PEEIPELVAAMA"
     gene            complement(4726..5859)
                     /gene="sucC"
                     /locus_tag="AC162G_01020"
                     /pseudo
     CDS             complement(4726..5859)
                     /gene="sucC"
                     /locus_tag="AC162G_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
BASE COUNT         1121 a         1947 c         1927 g          916 t
ORIGIN      
        1 gcggccagcg cgtcgaggga aaatcaccag ggttgagctt gtccgattgt ccccatagga
       61 cgaaaggcgg gcgcgtgacg tgagcgaaca cctggtagtt cgcaggcact ggcgcagtcg
      121 ccctccaata cagggtgagc ggcgcaacct ggcctgcctc gacgtcgccg ggcggcagat
      181 cgaacccgag cagctcgacg tggccgccaa agctggcgcg ctgcgcgtgc tgagcagcga
      241 gcgcctggcc gggcggcgac gtgtaacgaa agcaagtgtc acagcggtcg acgatcaaga
      301 ctttgacggc gagaaatgcg gccaacagcg gcacaagcag gcgaatcgcc gatcggggcg
      361 acctctgcac agacgggcct gccattagat cggccgaagc gggcgacttg cgcaatcgag
      421 acaccgcgac gatggcagcc gcgaccagga ggcttccgag cgtgacgatc gcgccggccg
      481 ttcgcggcgc agtcgattcg aatcgaacgc cgaacgtgtg cgccgacgcc ggcacgtcga
      541 acaggatgaa gccgtcccca ccggatggcc gcagcggcac aggctttccg tccacgtagg
      601 cccgccagcc cggaaaagcg aagatgaaga atcgcgcggt gaagtcgtcc gaagattcga
      661 cgtcgtattc cgcatccaga tcgccgatcc gaaccaggct cctgcggacg cgtgcgccgg
      721 ctgcgctggc atagtcgaac cgatcgacgg cgcctcctgt gcgataggcg tcgatgagcg
      781 gctgcgccgg cgggggtgtc tcggcgaagg ttcgcggcag gaagtctccg gtcgacgtcg
      841 tgcccagtgc gatgccgctc aactcgaact cgacggcgcc gcgcggcgac gtgtcgccga
      901 actcggcgtc ccacgacggc ggatacatcg ccggcagcgc ggtcagcaga acgaggctga
      961 gcgcgacggc tgccgcgatc tcggcgcggc ggaaagtgga taccgtacag ccggcgcaca
     1021 ttgccagtgc gaaagcagcc agcccgagga agcgccacgg gaactggatc agcgcggcag
     1081 gcggcacggc ttcccaccac gcctgcgatg ccggcatcat gagaaatatc agcgcgagcg
     1141 aggcgagcac gaagaacgcc gcgatgcgcg cggccggctg cgatcgccgg cggaggatcg
     1201 ccagcggcgc aggcagcgcg agcagccact gcgcgaaacc gagattgtag atgaagtgcg
     1261 gcgccgtcgc gcccagatcg agcacgggcg agggacgcaa gagcgtgccc agctcgacaa
     1321 agtggttgcg gaaatcgaag tggcccggac cggcaacgtc gaggcgcacc gcgctccgct
     1381 cggcgaagaa cggcagccaa aacacggcgg tcaggccggc ggcgagcgcg actgccgcga
     1441 cgtcgcgcag ccagcgccct gccccgtcga cgaacacgcc gcgccaggca agccacgccg
     1501 cgagcaagcc ggcgccgatg agcgccatca agttgtggct gagcacgagc gccgccagcg
     1561 ccagcgccgc gcgcgcgata tcgcgagccg acgacaaggg gcgatcgaat gcgagaaata
     1621 cccagggcaa aatgccgagc gccaggaatt cggcggcgtc cccgcgcatg aaaggatcga
     1681 cgaacagcac gtagggcgag agaacgtagg cggctgcggc gacgacgccg ctcgctccac
     1741 cgaagtgccg ccgcgcgaag aagaatgcgc cgcatgccgc cgacacgaac gtcagcacga
     1801 ggacgatctt catggcgtcg acgatgtcca gaaccggtac gagcgcgatc gcgccaacgg
     1861 cataataagt gagcggcgcg tagtagttga agatcgggta gccgtagccg tagtaaaagt
     1921 tgggcgacca gcgtggatac tcgagcggac gctcggccaa cagccgctcg aattcggcgg
     1981 cgcggaagac gtgcagctcg agatcggtgt ggcgcggcag gcccggccga gtgacgagag
     2041 gcagcgccgc gatgagcgcg atcagcagca cggccaggag tgaggcgtcg acgggcgcga
     2101 ggaccttctt agacgcggat ggtagccact caggcatcat cgcgagttct tcatggccaa
     2161 gcacggcatc cggtctggca cacagggacg taccacccga tccgcgacag acgtgtcatg
     2221 ccgaggagga acgacgaggc atctctaccc cggcgtgctg cgttcgccgt ttggagtaga
     2281 gattcctcac tgcgttcgga atgacacgtc acaatcggaa attcgacggg cgggcagaat
     2341 aatgaaggct ttccgtgcac gtcattcact gggacttcag cacaacgggc agatatatat
     2401 ggcgtgtttt gacataggcg tactcgaggg ttcgcgtgac ggacagcgtg taatacgcca
     2461 tgtgcggatc gccggcagcg tccacggcga cggctgtgta accgcgcaca ggtcgagccg
     2521 tggtcacggt ctgcaccagc cacgtcgggc ccagcttata ggcataacgc gctttcccgc
     2581 cgaccgtatc cgccagatag cccacatgcg gaatgccgtc agtgtcgaac gccaacgaca
     2641 catattcccc cggatcgccg atgcggtcga ttgtttcact cagccagacc gtcccgctca
     2701 acacggcata gcgcaagccg gcgcctggct cgttataagc aatcgatggc cgcccttgcg
     2761 catccagcgc gatcgcggta tacgcggctt ccggcgaatc ggctacgaca gcggtcaccc
     2821 actgtgtccc gctgagcgtc gcataataca accgggtgac gacctgcgtc gacgacagag
     2881 gatcagggga ttcagacaga aaggcgaggt gaggcgcctg gccgctgatc accacggatg
     2941 gtgcagcgac cagcatcgac aggttgccga cccgcgcgat agcttccttc agccacgtgc
     3001 ctccctcccg ataagcatgc atcagcctga gcgtcgtaaa gttgaagtag acgatgtgcg
     3061 gtcgtccctg tccgtcgaac gccagcgccg gccgggaaaa accctgtccg ccaggcccgg
     3121 agcctgtttg atcgacgtcg ctgaagaccc aggtcggact gatgcgcgcg gcgtaccgaa
     3181 cgttgaggtt cacgttgccc aaataggcga cggccggtcg atccgacgcg tccaagacga
     3241 gcgacgtctc ggcccccgtt tgatggcgag ggtcgacaac ctccgtcatc cactgagtgc
     3301 cgctcaagac ggcatacttc agcacgccca gcccctgatc gaagtaactg acgtgcgggc
     3361 gaccttgcga atcgagccgc atcgacgctc cggggccggc attgggagaa tcgaccgtct
     3421 gaaccgtcca gtgcgaaggc acgatagcca gcgcagcggc aggcggcagg cgtgtcgccg
     3481 ccgccatcgt gacgagcagg gcaacgaaag ccaggcccag gctggccagt gccgtgaagg
     3541 tgcgtcgtat cattctcacg taatggcaac gaccagcagg cacggcatcc ccatgccgtg
     3601 cccctcgttc aagccatggc agcgaccagc tcggggatct cttcagggtg ctgcgcgaca
     3661 cgcacgccgt gccgctcgaa ggccgcgatt ttttcggcgg ccgtacccgt gccgccctcg
     3721 atgatcgccc cggcatgccc catgcgcttg cccggcggcg cggtccggcc ggcgacgaag
     3781 gccgtgaccg gcttggtcat gcgcgcgcta atgaactcgg cggcgcgctc ctcgtcgttc
     3841 ccgccgattt cgccgatcag cacgacgtga tcggtgagag gatcctcttc aaacatctcc
     3901 aacacgtcga ggaaggaggt gccgtagatc gggtcgccgc cgatcccgac gcaggtgctt
     3961 tgccccatgc cacgatttgt cagcgcggcg atcacctcgt aggtcagtgt gccgctgcgc
     4021 gagacgatgc cgatatttcc cgaggcgcag atctggccag ggataattcc gaccttggcc
     4081 acgccaggcg taatcaggcc ggggcagttt gggccgatca ggcgcgtgcc gcgccggtcg
     4141 agatacgcgc gcacgcgcac catgtccagc accgggatgc cttcggtgat gcacacgaca
     4201 agagggatgc ccgcgtcggc cgcttccagc atcgcatccg gcgcggagcg cgccggcacg
     4261 aaaatgaccg acgcgttcgc gccggtggcg tccacggcct ccttgcacgt gtcgaagacc
     4321 ggcacgccgg cgacccactc gccgcccttt ccgaaacgca caccgccgac cacgttcgtg
     4381 ccgtattcga ccatcttgcg cgtgtgaaac tcgccttcgc gtccggtgat gccctggaca
     4441 atcaggcgcg tattcttatc gacgagaatg ctcatcaagc cttcctcatg gcgtatagct
     4501 gatagcacat agcaagtcgt cagctcaaga cttccgaaga cttggtcgtt tcttgccggc
     4561 gcctttcgcc gtcgaccgct tgctggtctg cgccggcttt cgagcggccg ctttcttcgc
     4621 cgggcgctga ccggcagcct tttttctgga cggttcaggc ggcgccttgg ctcgacgcgc
     4681 gggcaagggc gcggcagccg cggtcggcag atcgatcaca gcctcttcgc cgcgcgccaa
     4741 cgtcaccgcc tttttaatgc cctcgaccag cgtgacggcc gtctccatgc gcgcctcggc
     4801 caaaatgcga cgcccttcct cttcgttcgt gccgaccagg cgcgccacca tcggcaccgc
     4861 cggattcacg tcgcgcagcg ccgccacgat gccgcgcgct acctcgtcgc agcgcgcgat
     4921 gccgccgaag atgttgaaca gcaccgcctt gacgttcgga tcggcgagga taatgcgcag
     4981 cgcggctgcg actttatcgg actgcgcgcc gccgccgatg tccaggaagt tggccgggct
     5041 gccgccgaac agcttgatca aatccatcgt cgccatggcc aaaccggcgc cgttgaccat
     5101 gcagccgatc tcgccgtcga gctggatgta tgacagcccg cgctggcgcg cttcggcctc
     5161 ggacggcgtt tcttcgctca cgtcgcgcat ggccgccagg tcgggatggc gaaacagcgc
     5221 attcgcgtca agcacgatct tcccgtcgag cgcgacgagc ttcttgccgc ccgtgacgac
     5281 gagcgggtta atctcggcca ggctcccgtc gacgtcgagg aaacactggt acaggccgcg
     5341 cgcaatcgtc acgaagtcgc gcaccaggtc tcgatgcaga ccaatgccgg cggcgatctg
     5401 ttgggcctga taatcgcgca gccccaggaa cgggtcgatc gcagtgcgca cgattttttc
     5461 gggcgtgtct cgggcgacct cctcgatgtc cacgccgccc gccgacgacg ccatcacgac
     5521 ggcgcggcgc tgcgcacggt cgatgaccac gccaagatag atctccttcg caatatccgc
     5581 cgcttcgtcg acgagcactt tgcgcacggg cagccctttg atctccatcg agagaatctc
     5641 ggcggcgatg cgctcggctt cgtcgggatc gcgggccagc cgaatgccgc cggctttgcc
     5701 tcggccgccg accaggactt gcgccttgat gacggcgcgc ccgccgagct cgcgcgcgat
     5761 gcgccgggca tcttcaggcg tcgtcgccac ctcacctttg agaatcgcga tcccgtgctg
     5821 ggcgaacagg gacttggact ggtattcgtg aagcttcacg gaatggcccc cgctgagaag
     5881 tagccgcgcg gcgattatac catgcggtcg g
//
LOCUS       DBNHXB010000025        13490 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3696613, whole genome shotgun sequence.
ACCESSION   DBNHXB010000025 DBNHXB010000000
VERSION     DBNHXB010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 13490)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13490)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13490
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3696613"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="AC162G_01025"
     CDS             complement(<1..810)
                     /locus_tag="AC162G_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074428.1"
                     /translation="MQLIAWLLALAALTLGLYIFFLNVRHTANRHAGALLLLFAAHLL
                     AVGALTGAADAGQAAWPSVVLAATTVVQPALLIVTLVLIKPEWLHERRWLRWLLYGPA
                     ALPAALTAIDLVLGAQLWYTGLDAATYSGGFVPLSAYVNGRLALMFRVTLNYLVAVAT
                     ILFLAVVVLSRSVKSPTSRLNLARILLGVQITAIVIPIVLGQLSSGPVLTSLLGALFV
                     VGYAYAASWQMISERRLQTGRLQPRLTALILSTTIPLVGAVSLIVGVVVQQA"
     gene            complement(834..3551)
                     /locus_tag="AC162G_01030"
     CDS             complement(834..3551)
                     /locus_tag="AC162G_01030"
                     /inference="COORDINATES: protein motif:HMM:NF017078.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HIR5074429.1"
                     /translation="MTLRLKTLLLVSAALVGLIVAAYLTVSLVLQAGFADVEAENTRQ
                     NVRRALEGLSQKIAGLDKSARDYAGWDDTYAFIEDRNQDYVDTNLSDETFANNELSLM
                     MFVNLGGEVVFAKAYDLHGEVEVPIPPGLLDQLPALGRLTRHESVDSGLAGVLLLSDG
                     PLLVASRPILTSEYEGPIHGALIMGRNLDTALVHELAAITRMDLTLARLDDPQLPQDI
                     QRAVSVLSGTEQIHVEALSEESIAGYTLLSDISGQPRLVLRVATPRPVYQQGQRSLQA
                     ILLSITGLGLVFVVLVLLLLERSVLHRLARLRDVVQRLGQGGVSPTHQALEGSHELAQ
                     LSRREPADEIGTLTRVLNHMSGQLLDLIASLETRIQARTAQLLASADVGHAAASILDT
                     DRLLREVVTSISSRFGFYFTAVFTLDQAGKYAVLREATGEAGRTLKERGHKLEVGGES
                     MVGYVTAQGNPRVALDAAKEPLHFANPLLPDTRSEIALPLVVGDRVLGALDVQSTQEA
                     AFDEASVAVLQSMADQVAVAIANAQSFETIQRALDTTTRLYEAGRALFAAMMDGDAYE
                     AARRAWTSASMPDLDRLGIFMISQRDETDSPVEYWAAAEWDPRSGSPLLPSATGEGPG
                     VRAGARYRPDEMPLTRLVTGDAVLVVRDADDPRLPAPARLAMQQAGVKAALVIPLLIR
                     GRLEGLVIGTARQPIDLREDDVRFMQAVAEQLSVVLGALRAKEETQAALARVEHLNRH
                     LVGEAWRSYLALRPGGLAVESGDSPRPSSATGEGPGVRGVSEEASRLAIPIVVRGRTL
                     GALALEDDDPARQWSDEEWDLLTTVAGEVAQMLENARLLEQTQLRAARESQLNEIARK
                     IRRTDDIDLILKIAAEELGQALDASHARAMLGAPADLLGSPDGHDPPSV"
     gene            complement(3548..6388)
                     /locus_tag="AC162G_01035"
     CDS             complement(3548..6388)
                     /locus_tag="AC162G_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5074430.1"
                     /translation="MSQEVLKNRLEELFTSPGGPPPTGPLTLDGKPAPAGPKPALSEA
                     EGAVVSARQTHEGVYRALNRVSQTLNRLAAPSEILELIYTAAGEVLDNRNFSIALLDE
                     ANRSLSFPICTIDGARREVGSHPMGNGLAEYVIRTRAPLFLARDVQPELQKRGIDEIE
                     PPAHSLLSVPLMAGDKLVGVVTLQDYENVDVYTTEHLELLTILASQAAGALEHARLFE
                     RMSAALAETQRVGQDLVKFKLGIERSTEAMFMTDIEGRIVYANPAFEKVYGFGVEEAL
                     EKTPRIIKSGLIPQERYKEFWDALLSKQVVAGEIVNKTKDGRFILVDGSNNPILDDAG
                     NLLGFLAIHRDITERKRAEEALSRRAVQLQTAAEVSRAASSILNPDELLPRVVDLIRD
                     AFDLYYVGIFLVDLDGQHAALRAGSGEAGRLMLERGHRLAIGDSSMIGWCIAHCEPRV
                     AQDVGGETVRFDNPLLPETRSELALPLVARGDVLGAMTVQSRLASAFSEQDISVLQTM
                     TDQVAHAIANAQLFEQESRRSTELSVLNEMGRTLASVLEVDAVYELIYEYTARLMDAS
                     NFFIALYHEEEGQISFPIAYNDNQRISVPSRSLRSGLTDYILRHRQPLLLSEDVPAHM
                     ERLGIEFLALGNATPALSWLGVPVNYGDRILGAIVVQSVTAPALYRENDRDVLTAIAS
                     QTAVALENARLFEQARRQLDHLATIQQTLSALTATYTREEMIDALLPRVAGAVRADTV
                     SMFLIEGEHLIRVGRYPLDDEGNAAPRVGQAIALADYPLTQRVIETRRPLAFLADDPR
                     LQEHARQAFRAAGITASATIPLVGREGVLGTLAVNLSQPGRAFSQDDLGLLQTLADQA
                     TVALEKVRLLEETRHLAQHRQLINQVTGKMRSAMTVDEVLRIAVDELRQATQATRAVA
                     RLSPPAPAHDNGQTGRTDVQALGDAPGQETAP"
     gene            complement(6524..6961)
                     /locus_tag="AC162G_01040"
     CDS             complement(6524..6961)
                     /locus_tag="AC162G_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5074431.1"
                     /translation="MPTEAVATRAAIKPSDAHVLIVEDNIQNFVLLARLMAFLGVRQC
                     QWKASGWQVLEFADSMPQVDLILMDIHLPYEDGFEAMLRLRGEPRFRDTLIVAVTADA
                     NESTMSRAREAGFDGFIGKPLDPDRFPDQIKKILGGEAVWSIA"
     gene            complement(6951..7337)
                     /locus_tag="AC162G_01045"
     CDS             complement(6951..7337)
                     /locus_tag="AC162G_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5074432.1"
                     /translation="MDGKCILYIEDNDTNRMLVRRVLTAEGYTLLEAADGPAGVRAAE
                     EHVPDLILMDINLPELDGYQVTSHLKSNPQLSHIPVIAITANVMQGDREKSLDAGCDG
                     YIQKPIDVDRLPEQVANFITRSRHAH"
     gene            complement(7589..8422)
                     /locus_tag="AC162G_01050"
     CDS             complement(7589..8422)
                     /locus_tag="AC162G_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5074433.1"
                     /translation="MNTPVGSTLLSGAHLMVVDDERTTRLSLSEIFTLRGAHVVTAAD
                     GQEALNKLRESTVDLMVLDLKMPGMSGLQVAEEAQRLSPGTVIILLTAHASVDSAIGA
                     LRQGVFDYILKPAQPRAIVEVVERALVKRQEYLRRQNLVGLMEQTVNALRGGEPPAAD
                     RVQKMPPAGAAQVGPIRVDLQRREAFVLDQPLVLTPTEFDTLAYLVQHPDRVVSCREL
                     VRAVHGYDTTEHEARPIMRVHIHRLRQKIETDPEHPERLLTVRAAGYMLSAEPRPRPG
                     A"
     gene            complement(8520..12752)
                     /locus_tag="AC162G_01055"
     CDS             complement(8520..12752)
                     /locus_tag="AC162G_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5074434.1"
                     /translation="MSSKPAFTDVTILAVDDDAEILEMLGYIFKRHGYRMRQAFNGRD
                     ALDMLAADPSIDVILLDLLMPGDLNGMDTLRVIRADPALQQVPVLIVTALDSTEQQVA
                     GLDAGAIDYIAKPIQPRELIARVNAALRVSRGQRALRGAEERYRLLVEAARDLVFAVD
                     GAGRTTYVSPSSLAILGYFPEVFTTGQVSLLDLTHSDDRQRVGEMFALALQGVSGDDV
                     QFRVRRRDGEERWLSVSWAAMHDAAGRPVGAQAMARDVTLRKRSEAAIYQRSQELAAL
                     NLIASRVTQSLDLNTTLSDALDALMEVLSIDYGALYVVEGGRATARAWRGLGDDALDR
                     VRSLALSDAPWLDRFGVCDTWPEASIAPFDTLARLLRAPAWLTAPLRERETLQGAIVL
                     GSRSAGRFGENEVILVRTVADQIGVAITNSRLYEETRRRVDELALLNEVGRALTSTLD
                     LNEVLSVIMEEAVGVLQGEAGSVLLLDEMRDELVFASSVGPAADQLPGLRLPSGAGIA
                     GEALRSGYPLLVADTQNDPRFYRNVDALTGMVTRSLMAAPLRARGRMIGVMEVLNKRG
                     GHFTPNDVRLLDSLAQTAATAIVNAQLFEREQHRAHQLAALNEIGQRIASSLEADALL
                     PEVARLIAVRLGYAFAAVGLVDENGLDLVLHGVARGSGEAGTAETSLGIGMRLPLNQG
                     AIGRVVTQGRSLIVNEAQVQPDDLLGRGRGAGGSLLVTPITLEGIVIGVILVESQPRQ
                     VVSVIDVTTLDAVANQLAASIANSRLYAQVRRRNRELTAMHAISAAVSQSLELQNVLD
                     AALTLAQPLFEADGCRICLIEGTDLVYRAGYGPLNEELPEPRRKPIHGSFEGLAARQG
                     SVEILPDVPALRSRRPKGVGTPTLNDSEGEGSGADPDWRETWRVPTARPLGAIVALPL
                     WGHDRVQGVMTLMWREPRPFAEGNRQLLAAIGQQIGVAIDRARLYEDTRRRERELGAL
                     NDILRSVTSTLDLEQVLSAAMQGVRDVMGVEIGSLLLVDDADGSLLFRKALGPAGDAA
                     ALVDQHLAPEEGIAGWVVAHRQSLRVNDAPSGAPYARRFEQATGMQVRSIMAAPLVVK
                     ERTIGAIEVVNKPDGMSEADERLLNALAASIAVAVDNARLYQELARSARELERSHAQL
                     VQSEKLAATGRLSLSLAHEINNPLQAIANCLHLSLEPGLSEERRHEFLSMAREEVDRL
                     SVLVQRMLEFYRPSPGDQTTSSVNSAIARVLALAEPKLRRNAVEVLTDLAPDLPEVRI
                     AGDQLTQVFLNLVVNAAEAMETTRGARLAVRSRLDESGDWVEVTFADNGPGIPLDVLP
                     HVFEPFFTTKAAGSGLGLAVSYGIMERHGGVLAVQSFPGHGTTFTARLPAGSGAASPD
                     LSLAHVGINAPTDVTLGGAKDLSPSGRDASLR"
     gene            13065..>13490
                     /locus_tag="AC162G_01060"
     CDS             13065..>13490
                     /locus_tag="AC162G_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074435.1"
                     /translation="MKINIIGAGPAGLYFAILMKKIDPAHDVTIVERDGPNDTFGWGI
                     VFSDKTFAFLKDSDETSYLDITAAAESWDNVDVVHRDQRISVRGNEFLGIARITFLNI
                     LHKYCRNLGIDLRFHTNVTTPDRIAELADCDLLVGGDGAN"
BASE COUNT         2364 a         4348 c         4543 g         2235 t
ORIGIN      
        1 ggcctgctgg acgacgacac ccacgatcag ggatacggca ccgacaaggg ggatggtggt
       61 cgacagaatg agcgcggtca ggcgcggctg caggcggccc gtctgcagcc gacgctccga
      121 tatcatctgc catgatgcgg cgtacgcgta accgacgacg aagagtgcgc cgagcaggga
      181 tgtgagcacc gggccggaac tgagctgacc gagtacgatg gggatcacga tagcggtgat
      241 ctgcacgccc aacagaatac gcgccaagtt taagcgcgaa gtgggggatt tcacggatcg
      301 gcttaggacg acgaccgcca gaaagaggat ggtggcgact gccaccagat agttgagagt
      361 taccctgaac atcaacgcca ggcggccatt cacgtacgca gagagtggca caaatccgcc
      421 actgtacgtc gcggcatcca gaccggtgta ccacaattga gcgcccaaca ccaggtcgat
      481 tgcggtgagc gccgccggaa gcgcggcagg cccatacaga agccagcgca gccagcgtcg
      541 ctcgtggagc cactcgggct tgatcaagac gagcgtgacg atgagcagcg ccggttgcac
      601 gacggtggtg gcggccagca cgacgctcgg ccacgccgcc tggccagcat ccgccgcgcc
      661 tgtcaaggcc cccacggcaa ggagatgtgc cgcaaacaac agcagcaaag cgccggcgtg
      721 gcgattggcc gtgtgcctta cgttgaggaa gaagatgtac aggcccaggg tgagggcggc
      781 gagagcgagc agccaggcga tcagttgcac ggtgtcacac ggatttcctc ggattacacg
      841 gaaggcgggt catgaccatc gggcgagccg aggagatcgg ccggcgcgcc caacatggcg
      901 cgcgcgtgcg acgcatccag ggcttgccca agctcctcgg cagcgatttt gagaatgagg
      961 tcgatatcgt ccgtccggcg tattttccgg gcgatctcgt tgagctggct ctcgcgcgct
     1021 gcacgcaact gggtctgctc gagcaagcgg gcgttctcca gcatctgggc gacttccccg
     1081 gccacagtcg tcagcaagtc ccactcctcg tcgctccact gccgggcagg gtcgtcgtct
     1141 tcgagggcga gggcgcccaa ggttcggccg cgcacgacga tggggatggc aaggcggctg
     1201 gcctcctcgg agacgcccct cacccccggc ccctctcccg tcgcagacga cgggcgaggg
     1261 gagtcgccgg actcgacggc cagcccgccg ggccgcaaag ccagatagct gcgccaggct
     1321 tcgccgacga gatggcggtt caagtgctcg acgcgcgcca gcgccgcctg ggtctcttcc
     1381 ttggcgcgga gcgctccaag gacgacggag agttgctcgg cgacggcctg catgaagcgc
     1441 acatcgtcct cgcgtaaatc gatgggctgc ctcgccgttc caatgacgag gccctccaag
     1501 cggcctcgga tcaggagggg aatcacaagc gccgccttca ctccggcctg ctgcatagcc
     1561 aggcgcgcgg gcgccggcag tcgcggatcg tcggcgtcgc gcacgaccaa aacggcatcg
     1621 ccggttacga gccgagtcag cggcatctcg tccggccgat agcgagcgcc ggccctcacc
     1681 cccggcccct ctcccgtcgc agacggcagg agaggggagc cggagcgcgg gtcccactcg
     1741 gcggctgccc aatattcgac gggactgtcg gtctcatcac gctgcgagat catgaagatg
     1801 cccagccgat ccagatcggg catagaggcg gaggtccagg cgcggcgggc cgcctcgtaa
     1861 gcgtccccgt ccatcatcgc cgcaaacaag gcgcggccgg cttcgtacag gcgcgtggtc
     1921 gtgtcgaggg cgcgctggat ggtctcgaac gactgggcgt tggcgatggc gacggccacc
     1981 tggtcggcca tggactgcaa cacggcgacg ctggcctcat cgaaggcggc ttcctgcgtc
     2041 gactgcacgt cgagcgcgcc caggacgcga tcgccgacca ccagcggcag ggcgatctcg
     2101 gagcgtgtgt cggggagcaa gggattggcg aaatgcagcg gctctttggc tgcatccagc
     2161 gccacgcgcg gattgccctg tgccgtcacg tagcccacca tcgactcgcc gccaacctcg
     2221 agtttgtgac cgcgctcctt caatgtgcgg ccagcctcgc cggtcgcctc gcggagcaca
     2281 gcatacttcc cggcctggtc gagggtgaac acagccgtga agtagaagcc aaagcggctg
     2341 ctgatcgagg tgaccacctc acgcagcagg cgatcggtgt cgaggatgga tgcggccgcg
     2401 tgaccgacgt cggcgctggc aagcaactgc gccgtgcgcg cctggatgcg tgtctccagc
     2461 gacgcgatga gatcgagcaa ctggccggac atgtggttga gcacgcgcgt gagcgtgccg
     2521 atctcgtctg ccggttcgcg gcgggacagc tgcgccaact cgtggctacc ttccagcgcc
     2581 tgatgggtgg gcgagacccc cccctgaccg agccgctgaa caacgtcccg gaggcgggca
     2641 agcctatgca gaaccgatct ctccaggaga agcaaaacca gcacgacgaa gaccaaaccg
     2701 aggcccgtga tggagagcag aatggcttga agactgcgct gaccctgctg gtataccggc
     2761 ctgggggtgg ccactcgcag aaccaagcgc ggctgcccag agatatcgga cagcagtgtg
     2821 tagcctgcaa tggactcttc gctcagcgcc tcgacgtgga tttgttcagt tcccgacaac
     2881 acggacacgg cccgttgaat gtcttgcggc agctgcggat catcgagtcg cgcaagggtc
     2941 aagtccatgc gcgtgatggc tgccagttcg tgcactagcg ccgtgtccag gttgcgaccc
     3001 atgatcagcg cgccatgaat cggcccctca tactcgctcg tgagaatcgg gcgcgaggcg
     3061 acgagcaatg ggccgtcgga tagcagcagc actccggcca atccgctgtc gacggattca
     3121 tgccgcgtga gccggcccaa ggccggcaac tggtcgagca gtcccggggg gattgggacc
     3181 tcgacttcgc cgtgcaggtc gtaggccttg gcgaagacga cttcaccgcc gaggttcaca
     3241 aacatcatca ggctcagctc gttgttggcg aaagtctcgt cgctgaggtt ggtgtcgacg
     3301 taatcctggt tgcgatcttc aatgaaggcg taggtgtcgt cccagccggc gtagtcgcgg
     3361 gcggacttgt ccagtccggc aatcttttga gacagcccct ccagagcacg ccggacgttc
     3421 tggcgcgtgt tctccgcttc cacatctgca aagccggcct gcagcaccag cgacacggtc
     3481 aaatatgcgg cgacgatcag gccgacgagc gccgcgctga ccagcagtaa ggtcttgagg
     3541 cggagcgtca tggagccgtc tcctgcccag gcgcgtcgcc gagcgcttgc acgtctgttc
     3601 ggccggtttg gccattgtcg tgcgccggcg cgggtgggct gaggcgagcc accgcgcgcg
     3661 tggcctgggt ggcctggcgc aattcatcca cggcgatgcg cagcacctca tctacggtca
     3721 tcgccgaacg catcttgccg gtgacctggt tgatcaactg ccggtgctga gccaggtggc
     3781 gcgtctcttc caagagccga accttctcca aggcgaccgt ggcctgatcg gccagcgtct
     3841 gcaacaagcc gaggtcatcc tgactgaacg cacgtcccgg ctggctcagg ttgaccgcca
     3901 gtgtccccag aactccctcg cgaccgacga gtggaatggt ggcgctggca gtgatgccgg
     3961 ccgctctgaa ggcctgccgg gcatgctcct gcaatcgggg atcgtctgcc aggaaggcca
     4021 gcggccggcg cgtctcgatc acccgctgtg tgagcggata gtcggccagc gcgatggcct
     4081 gcccgacgcg cggcgcggcg ttgccctcgt catccaacgg ataccggccg acacgaatca
     4141 gatgttcccc ttcgatcaga aacatgctga ccgtatcggc gcgcacggcg ccagcgacac
     4201 ggggaagcag agcgtcgatc atctcctcgc gcgtataggt cgcggtgagc gcagatagcg
     4261 tctgctggat cgtcgccaga tggtcgagtt gtcgccgggc ctgctcgaac agacgcgcat
     4321 tctcgagcgc gacagcggtc tggctggcaa ttgccgtgag gacgtcgcgg tcattctctc
     4381 gatagagagc gggtgccgtg acgctctgca cgacgatcgc gcccaggatt cgatcgccat
     4441 agttcacggg cacacccagc caagataggg caggcgtggc gttgcccaag gcgaggaatt
     4501 cgatgcctag cctctccatg tgagccggga cgtcctcgct gaggaggagc ggctggcgat
     4561 ggcggagaat gtagtcggtc agcccggagc gcagcgagcg cgaaggcacg ctgatgcgct
     4621 ggttgtcgtt gtaagcaata ggaaacgaaa tctgcccttc ttcttcgtgg taaagcgcaa
     4681 tgaagaagtt ggaggcgtcc atcaggcgcg ccgtatactc gtaaatcaac tcgtagacgg
     4741 cgtcgacttc gagaacagag gccagcgtgc gccccatttc gttcagcacg gatagttccg
     4801 tagagcggcg gctttcctgc tcgaagagct gcgcgttggc gatggcatgg gcgacctggt
     4861 cggtcatcgt ctgcagcacc gagatatcct gctcggagaa agccgacgcc agacggctct
     4921 gcacggtcat cgcgccgagc acgtcgccgc gcgccacgag cggcagcgcc agctcggatc
     4981 gggtctcggg cagcagggga ttgtcgaagc gcaccgtctc gccgccgaca tcctgggcca
     5041 cccgcggctc gcagtgcgcg atgcaccagc cgatcatgga cgagtcgccg atcgccagtc
     5101 ggtgcccccg ctcgagcatc agccggccgg cttccccgct gccggcgcgc agcgcggcat
     5161 gctggccatc gagatcgacg aggaagatgc cgacgtagta gaggtcgaac gcgtcgcgga
     5221 tgaggtcgac gacgcgcggc agcagctcgt cggggttcag gatagaagac gcggcgcgcg
     5281 agacttcggc ggcggtctga agctgcacgg cgcgccggga caacgcttcc tcggctcgct
     5341 tgcgctcggt gatatcacgg tgaatggcca ggaagcccaa gaggttgccg gcgtcgtcca
     5401 ggatcgggtt gttggagccg tcgacaagga tgaaccgccc gtctttcgtc ttgttgacga
     5461 tttctcctgc cacgacctgc ttgctgagaa gagcatccca gaattccttg tagcgctcct
     5521 gggggatcaa gccggacttg ataatgcgag gcgtcttttc gagggcttcc tcgacgccga
     5581 atccgtagac tttctcgaag gccggattgg catagacgat acggccctcg atatccgtca
     5641 tgaacatcgc ctccgtcgag cgctcgatgc caagcttgaa cttgacgagg tcctgtccga
     5701 cccgctgcgt ctccgcgaga gccgcgctca tgcgctcgaa caggcgggcg tgctcgagcg
     5761 cgccggccgc ctgtgacgcc aagatcgtga gcagctccag atgctccgtc gtatagacgt
     5821 cgacgttctc atagtcctga agcgtgacga cgccgacaag cttatcgccg gccatcaagg
     5881 gcacgctcag caggctgtgg gccggcggct cgatctcgtc gatgccgcgc ttctggagct
     5941 ctggctgcac gtcacgcgcc aggaagaggg gcgcgcgtgt gcggatgacg tactcggcca
     6001 gcccgttacc catggggtgg ctgccgacct cgcgacgggc gccgtcgatg gtgcagattg
     6061 ggaaggagag ggatcgattc gcctcgtcaa gcagggcgat cgagaaattg cgattgtcca
     6121 gcacctcgcc ggcggcggtg taaatcagct ccaagatttc ggacggggcg gccagccggt
     6181 tgagggtctg gcttacgcgg ttcagcgcgc ggtacacgcc ctcgtgtgtc tgccgcgcgc
     6241 tgacgaccgc cccttcggct tcgctcaggg caggcttggg gccagcaggg gcaggcttcc
     6301 catccagggt gagcggcccg gtgggcggcg gcccacctgg ggaggtgaat aactcttcta
     6361 atcggttctt caagacttcc tggctcatcg gtcatccgct ccgcggccga accgcgcccg
     6421 gggcgccgct ggcagatgcg gaaacacgcc ggttacgcgg ccgcacacat gggcatggcg
     6481 ctggcggcag caagtgtctc ccttcaaaag gagcgcgccg gaactaggcg atcgaccaga
     6541 ccgcttcgcc gccgaggatc ttcttgatct ggtcggggaa gcgatccgga tcaagcggct
     6601 tgccgatgaa gccgtcgaag cccgcctcgc gggcgcggct catggtggac tcgttcgcgt
     6661 cggcagtgac tgcgacgatc aaggtgtccc tgaaacgcgg ctcgccgcgc aggcgcagca
     6721 tggcttcgaa gccgtcttcg tagggcaaat gaatgtccat caggatcaag tcgacctgtg
     6781 gcatgctgtc ggcgaactct agaacctgcc agcccgacgc tttccactgg cactgcctga
     6841 cgccgagaaa ggccatcagg cgcgcgagca gcacaaagtt ctggatgttg tcttcgacga
     6901 tgaggacgtg agcgtcggat ggcttgatgg cggcgcgtgt ggcaacggct tcagtgggca
     6961 tgacgagacc tcgtaatgaa atttgcgact tgctcgggga gccgatcgac gtcgatcggt
     7021 ttctgaatgt atccgtcgca gccggcatcc aggctcttct cgcgatcgcc ctgcatgacg
     7081 ttggctgtga tggcgatgac cgggatgtgg ctgagctgcg ggttgctttt gaggtgggac
     7141 gtcacctggt agccgtccaa ttcgggcagg ttgatgtcca tcaagatgag gtcgggcacg
     7201 tgttcttcgg cggcgcgcac gccagccggg ccgtcggccg cttcgagcag ggtgtaacct
     7261 tcggcggtca gcacgcggcg cacgagcatg cgattcgtgt cgttgtcttc gatatacagg
     7321 atgcacttgc cgtccatgac ctcgctccgt ccgacgagac gcgcgatgag tccggccgat
     7381 ccggccaggt tcagaatacc atgcgcaaac tgtattgtga ctttcgagag cgttgcggga
     7441 cgcttgcgaa tacgtaatct gaagcgcgcc gcggcacgac acggatttcg gtcacggatt
     7501 gcacggtatg aggatgtggg gggcgcagac gccggagagg ggtccggcga ttgtcgatgc
     7561 gcaggggcgg gtcttgcgcc cgccccgatc acgcgccggg ccgagggcgc ggctcggctg
     7621 acagcatgta cccggcggca cgaacggtca gcaatcgctc gggatgttct ggatcggttt
     7681 caatcttttg acgcaggcga tggatgtgaa cgcgcatgat cgggcgggcc tcgtgctcgg
     7741 tcgtgtcgta gccgtgcacg gcgcgcacca gctcgcggca tgatacgacg cgatccgggt
     7801 gctgcaccag ataggccagc gtatcgaact cggtcggcgt gagcaccaga ggctgatcca
     7861 acacgaacgc ctctcgccgc tgcaagtcga cgcggatcgg gccgacctgc gcggcgccgg
     7921 ccggaggcat tttctgcacg cgatcggccg ccggcggctc gccgcccctg agcgcgttga
     7981 ccgtctgctc catcaggccg acgaggttct ggcgccggag gtactcctgg cgcttgacca
     8041 gggcgcgctc cacaacctcg acgatcgcgc gtggctgcgc cggcttgagg atgtaatcga
     8101 atacgccctg tcgcaacgcg ccgatggccg agtcaaccga ggcatgcgcc gtgagcagga
     8161 tgatgacggt gcccggcgac aggcgctgcg cttcttccgc cacctgcagg ccgctcatgc
     8221 cgggcatctt caagtcgagc accatgaggt cgacggtcga ttcgcgcagc ttgttgagcg
     8281 cttcctgccc atcggccgcc gtgacgacgt gcgcgccgcg cagggtgaag atctccgaga
     8341 gcgacaggcg cgtcgtgcgc tcgtcgtcca cgaccatcag atgcgcgccg ctcagcagcg
     8401 tgctaccgac tggcgtattc atcgtctgga ctcctcccat ccattcgcgg gtgattgcac
     8461 acaggtcatt atgccggtgt gtcatgctga gcgaagcgaa gcatcagcga agcgaagcat
     8521 cagcgaagcg aagcatctcg acccgatggg gagagatcct tggctccgcc tagagtgaca
     8581 tctgtcggcg cgtttatgcc cacatgcgcc agcgacaaat ccggcgacgc ggcgccggaa
     8641 cccgccggca agcgggcagt gaaggtcgtc ccatggcccg ggaagctctg gacggcgagc
     8701 acaccgccgt ggcgctccat gatgccgtag ctgacggcaa gccccagccc actgccggca
     8761 gccttcgtcg tgaagaatgg ctcgaagaca tggggcagca cgtcgagcgg aatgcccggg
     8821 ccattgtcgg cgaaagtgac ctcgacccag tcgcccgact cgtcgagccg gctgcggacg
     8881 gccaggcgcg cgccgcgcgt cgtctccatc gcctcggcgg cgttcacgac caggttgaga
     8941 aacacctgcg tcaactgatc gccggcgatg cgcacctcgg gaagatcagg agcgagatcg
     9001 gtgaggacct cgacggcgtt gcggcgcagc ttgggctctg ccagggccaa cacccgcgcg
     9061 atggcgctgt tgacgctgct cgtcgtctgg tcgccagggg aggggcgata gaactcgagc
     9121 atgcgctgga cgaggacgct cagccggtcg acctcctcgc gcgccatcga caggaactcg
     9181 tggcggcgct cctcgctcag gccgggttcg agcgagaggt gcaggcagtt ggcgatggct
     9241 tgcagcgggt tgttgatttc gtgcgcgagg ctgagcgaca gccggccggt ggccgccaac
     9301 ttttcggact gcaccaactg cgcgtgcgag cgctccagct cgcgcgcgga gcgcgccagc
     9361 tcctgataca accgcgcgtt gtcgacggcc acggcgatcg aggccgccag ggcgttaagc
     9421 agtctctcat ccgcttcgct catgccgtcc ggcttgttga cgacttcgat cgcgccgatc
     9481 gtgcgctcct tgacgacgag gggcgcggcc atgatactgc gcacctgcat gcccgtcgcc
     9541 tgctcgaagc ggcgcgcgta aggcgccccg gacggcgcgt cgttgacgcg cagcgattgg
     9601 cggtgggcga cgacccagcc ggcgatgccc tcctcgggcg ccagatgttg atcgacgagc
     9661 gctgccgcat cgccggccgg accgagggcc ttgcgaaaga gcagggagcc gtctgcgtcg
     9721 tcgacgagta gcagggatcc gatctcgacg cccatgacgt cacgcacgcc ttgcatggcg
     9781 gcgctcaaca cctgctccag atcgagcgtc gaggtgaccg agcgcagaat gtcgttgagg
     9841 gcgccaagct cgcgctcgcg gcggcgtgtg tcctcgtaca ggcgagcgcg atcgatggcc
     9901 acgccgatct gctggccgat agcggccagc aactgccggt tgccttctgc aaacgggcgc
     9961 ggctcgcgcc acatgagggt catgacaccc tgcacgcggt cgtgccccca caagggcagg
    10021 gcgacgatcg cgcccagcgg cctcgctgtc gggacgcgcc acgtctcgcg ccagtccgga
    10081 tccgcgcctg acccttcccc ttcactgtcg ttgagggtcg gggtccccac tcccttcggt
    10141 cggcgacttc gcagggcagg cacgtcgggc aggatttcga ccgatccctg gcgcgcggcc
    10201 aagccctcga aggagccgtg gatgggcttg cggcgaggct caggcagttc ctcgttcaac
    10261 ggcccatacc cggcgcggta caccaggtcc gtgccttcga tcaggcagat gcggcagcca
    10321 tccgcctcga acaggggctg cgccagggtg agtgccgcat ccagcacgtt ctgcagctcg
    10381 agcgattggc tgaccgccgc cgagatggcg tgcatcgcgg tcagctcgcg attgcgccgg
    10441 cgcacctgcg catacaggcg cgaattggcg attgacgcgg cgagctgatt ggcgacggcg
    10501 tcaagtgttg tgacgtcgat gacgctgaca acctgacgcg gctgggattc gaccaggatc
    10561 acgccgatga cgatgccttc cagggtgatg ggcgtgacca ggaggctccc gccggcgccc
    10621 cggccacgcc cgagcaaatc atccggctgg acctgggctt cgttgacgat caggctgcgg
    10681 ccttgggtga cgacgcggcc gatggcgccc tgattcagag gcagccgcat gccgattccg
    10741 agcgaggtct ccgccgtccc cgcctcgccg gagccgcgcg ccacgccatg caagaccaga
    10801 tcgaggccat tctcgtccac cagccccacg gcggcaaagg cgtagcccag gcggacggcg
    10861 atgaggcggg ccacctcagg caggagcgcg tcggcttcga gggaggacgc gatgcgctgc
    10921 ccgatctcgt tgagcgcggc cagttgatgg gcgcggtgct gctcgcgctc gaacagctgc
    10981 gcgttgacga tggccgtcgc ggcggtctgg gccagcgaat cgagcaggcg cacgtcgttc
    11041 ggcgtgaagt gcccgcctcg cttgttgagc acctccatca ccccgatcat gcgcccgcgc
    11101 gcgcgcagcg gcgctgccat gagcgagcgc gtcaccatgc ctgtcaacgc gtcgacgttc
    11161 cggtagaaac gcggatcgtt ctgcgtgtcg gccactaata gaggatagcc gctgcgcagg
    11221 gcttctccgg caatgcccgc gccgctcggc aggcgcagcc ccggcaactg gtcggcggcc
    11281 ggaccgaccg aggatgcgaa caccagctcg tcgcgcatct cgtcgagcag gaggacggag
    11341 ccggcctcgc cctgaagcac gccgacggct tcttccatga tcacgctcag gacttcgtta
    11401 aggtcgagcg tcgacgtcaa tgcgcgcccg acttcgttca acagcgccag ctcgtcgacg
    11461 cggcggcgcg tctcctcgta caggcgcgag ttcgtgatcg ccacgccgat ctgatcggcc
    11521 accgtgcgaa cgagaatgac ctcgttctcg ccgaagcgcc cggcggagcg gcttcccagc
    11581 acgatcgcgc cctgcagcgt ctcgcgctcg cgcagggggg cggtcagcca ggccggcgcg
    11641 cggagcaggc gcgcgagcgt gtcgaagggc gcgatcgacg cctcgggcca ggtgtcgcag
    11701 acgccgaagc gatccagcca cggcgcgtcg gagagcgcca gagaacgcac ccggtcgagc
    11761 gcgtcgtcgc ccagcccgcg ccaggcgcgc gccgtggcgc gcccgccctc gacgacgtaa
    11821 agtgcaccgt agtcgatgct gagcacttcc atcagcgcgt ccagcgcgtc cgaaagcgtg
    11881 gtgttgagat cgagcgactg ggtcacgcgc gaggcgatca gattgagcgc ggcaagctcc
    11941 tgcgagcgct ggtagatcgc cgcctcggat cgcttgcgca gcgtcacgtc gcgagccatc
    12001 gcttgcgcgc ccaccggccg cccggcggcg tcgtgcatgg ccgcccacga caccgacagc
    12061 cagcgctctt ccccgtcgcg gcgccgcacg cggaactgca cgtcgtcccc cgacacaccc
    12121 tgcaaggcca gcgcgaacat ttcaccgacg cgctgccgat cgtcggaatg cgtcaggtcc
    12181 agcaggctca cctgccccgt ggtgaacact tccgggaaat agccgaggat ggccagggac
    12241 gaggggctga cgtaggtagt gcgccctgcc ccgtcgaccg cgaacaccag gtcgcgagcg
    12301 gcctcgacca gcaggcggta tcgttcctcg gccccgcgca gcgcgcgctg gccacgcgag
    12361 acgcgcagcg cggcattgac ccgcgcgatc aactcgcgcg gctgaatcgg cttggcgatg
    12421 tagtcgatgg cgccggcgtc caggccggcc acctgctgct cggtagagtc gagcgccgtg
    12481 acgatgagca ccggaacctg ctgcagcgcc ggatcggcgc ggatgacgcg cagcgtgtcc
    12541 atcccgttca ggtcgccggg catcagcagg tcgagcagga tgacatcgat cgagggatcg
    12601 gcggcgagca tgtccagcgc gtcacggccg ttgaacgcct gccgcatgcg atagccatgc
    12661 cgcttgaaga tgtagccgag catctccaga atttcggcgt cgtcgtcgac ggccaggatc
    12721 gtgacgtcgg tgaaggcggg cttgctgctc ataggctgcc tgcaatggct gtgattataa
    12781 catcgacggg acgcgaggca cagagggggc cgttgatctc ccgcgccccc ttgccgaggt
    12841 tgtaatgaca tcgacctgat caatcgtcgg aggagtcaaa cgaggcaacc cggcctcacc
    12901 ctgcccacct gactcgctgg ccggcttggg gtgtgagcgc gctgagctta ctccgtggtg
    12961 agtgtcgatg atataatgtc gcccgcaagc gaggagcctt tctaagcagg ccgctgtcac
    13021 atgggcgggg cctgttcacg cgacattcgg gagatcgacg cactatgaaa atcaatatca
    13081 tcggcgccgg gccggccggg ttgtattttg cgatcctgat gaagaaaatc gatccggccc
    13141 acgacgtcac gatcgtcgag cgggacgggc cgaacgatac attcggctgg ggcatcgtct
    13201 tctccgacaa gacgttcgcc ttcctcaaag acagcgacga aacatcctac ctcgacatta
    13261 cggccgccgc cgagagctgg gacaatgtgg acgtcgtcca tcgcgatcag aggatcagcg
    13321 tgcgcggcaa cgaattcctg ggcatcgccc gcatcacttt cctgaacatt ctccacaagt
    13381 actgccggaa cctgggcatc gatctccgct tccacacgaa cgtcaccacc cccgatcgca
    13441 tcgccgagct ggccgattgc gacttgctcg tcggcggcga cggcgcgaac
//
LOCUS       DBNHXB010000026         3651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4199935, whole genome shotgun sequence.
ACCESSION   DBNHXB010000026 DBNHXB010000000
VERSION     DBNHXB010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4199935"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="AC162G_01065"
     CDS             <1..998
                     /locus_tag="AC162G_01065"
                     /inference="COORDINATES: protein motif:HMM:NF025067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HIR5074436.1"
                     /translation="LLFVFGGLFVAADAVFEDFVARLFDWDVDEVVTRTILISFWTIL
                     AGGFLRRTLMAKEWTGSAFRQPKSLSLGSVEIGVALGMLNALFLAFVVVQVRYLFGGA
                     GVVESSIHLTYAEYARRGFFELVAVAALVLPTLLVAHWLLPEGDTAARRLFSLLAGAL
                     IGLLFVIMASAVQRMLLYQREFGLTELRVYTTAFMGWLAIVFVWFAVSVLRGRRERFA
                     FGALAAGFTVILALHALNPDDLIARVNTNRPDAAVPFDARYVTALSADAVPALVEALP
                     LMNERDRCLVAAHLLAGWSPPATPDWRTWNFSRAQAWATVDANRAYLQSVACPGR"
     gene            1186..2694
                     /locus_tag="AC162G_01070"
     CDS             1186..2694
                     /locus_tag="AC162G_01070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR5074437.1"
                     /translation="MKSHRWIVATLCALAFALQLGPAMQNRFHPDEALYTAWAMQIVS
                     GRDALLSGAPVDKPPLSMYIMATSLWALGRSEIAARLPNLIAATLSVALVWQWAKAFY
                     PARSRAGEEGGGVVAALAVALSPFTIAFGGTAFLDPLMVMWGLAACVTASRGRPGWAG
                     IFLGLSFATKVQGLLFAPLVILTAQLAESAERWKRRISTRSASSAAKQCLSSASGFGI
                     GVALVIAWSLLRSGTPFWEQQAINYGGLRFAFAAELGPRLISWSGFLPHLFGPILGGI
                     LVVCLISLLVHAFGHGKGTRGAAIDLLLVAYIVGFVALHWLLAFPVWDRYLLILVPVA
                     GVLLDRSVAVTLAWLSSHSHVAIGRSSALAVAAIGTIVLPFALQAAHSEILIGGDHGP
                     HDGIDRAAAYLRELPVGTVVYDHWLSWEFDYYLWDAPLYRAYFDTPADLARDLRVFGR
                     SSTRYLVVPANESLGKIERAVADAGFRIAPVMKSADRLGRTAFVVYRTVLRE"
     gene            complement(2727..>3651)
                     /locus_tag="AC162G_01075"
     CDS             complement(2727..>3651)
                     /locus_tag="AC162G_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5074438.1"
                     /translation="AYHGATAGAISLTGDPRRWANEPGLPGIVRVFDPYKYRSPLYQP
                     GDSDQVFARKCLDQIEEVIMYEGAHTIAAIFIETVTGTNGLIVPPDGYLQGLREICDR
                     HGILLICDEVMCGLGRAGEWFAVDHWNVVPDIITMAKGLTSAYMPLGCVALSEKIAHY
                     FDDRVFSGGLTYNGHPMCLAAAVANLKVLEEDDLVGNARRMGNVMAGLLDDLKAEHPS
                     VGDVRSIGLFGIVELVKSRKTKEPLAPFDGTSEPMQKLAAFFKQKGLYIFTHSNTFFT
                     NPPLCITEAQLKEVFEIINEGLAIADGAVVA"
BASE COUNT          535 a         1145 c         1190 g          781 t
ORIGIN      
        1 tcctgctgtt cgtcttcggc ggcctgttcg tcgcggcgga cgccgtgttc gaagacttcg
       61 tcgcccggct gttcgactgg gacgtcgacg aagtcgtgac gcgcacgatc ttgatctcgt
      121 tctggacgat cctcgccggc ggtttcctgc gccggacttt gatggcgaaa gaatggaccg
      181 gttccgcctt caggcagccg aagtccctgt cactcggcag tgtcgagatc ggtgtcgcgc
      241 tcgggatgtt gaacgcgctg ttcctcgcct tcgtcgtcgt gcaggtgcgt tacctcttcg
      301 gcggcgcggg cgtggtcgag tcgtctattc atctgactta cgccgaatac gcccggcgcg
      361 gcttcttcga gctggtcgcg gtcgcggcgc tggtcctgcc gacgctgctg gttgcccatt
      421 ggcttttgcc cgaaggcgat acggcggccc ggcggctgtt cagcctcctg gccggcgcgt
      481 tgatcggcct gctgttcgtg atcatggcct cggccgtgca gcgcatgctg ctgtatcagc
      541 gcgagttcgg cctgaccgag ctgcgcgtgt acacgacggc gttcatgggc tggctggcga
      601 tcgtcttcgt ctggttcgcc gtcagcgtgc tgcgcggacg gcgggaacgc ttcgccttcg
      661 gggcgcttgc ggcaggcttc accgtgattc tcgcgctgca cgccctcaac cccgatgact
      721 tgatcgcccg cgttaacacg aaccgcccgg acgcggccgt cccgttcgac gcgcgctacg
      781 tcaccgcgct cagcgccgac gccgtgcccg cactcgtcga agcgctgccg ctgatgaatg
      841 agagggatcg gtgtctggtc gccgcgcacc tgctggcggg atggtcgccg cccgcgaccc
      901 ccgactggcg cacctggaac ttcagccgcg cgcaggcgtg ggccaccgtc gacgcaaatc
      961 gagcctactt gcagagcgtc gcgtgcccgg ggcggtagca ttcgcacatg agatcagcgc
     1021 gctggtggag ctggccttgc catttaagcg tcattcgttt agagtcgcag ttggacggcg
     1081 gctctttttg tttgttgtca gccaacttca caggcgagtc agggcgtcgc tgcaccttcg
     1141 tcgtgatgat gacagagatt gactttgagc cttcgtgtct ttgtggtgaa atctcataga
     1201 tggatcgtcg ctacattgtg cgccctggcc ttcgcgcttc agctcggccc ggctatgcag
     1261 aaccgctttc atcccgacga ggcgctatac accgcctggg cgatgcagat tgtgagcggg
     1321 cgtgacgcgc ttctctccgg cgcgcccgtc gataagccgc cgctgtcgat gtatatcatg
     1381 gcgaccagcc tgtgggcgct cgggcgcagc gagatcgcgg cgcggctgcc aaacctgatc
     1441 gccgctacgc tcagtgtcgc gctggtgtgg cagtgggcca aagcgttcta cccggcgcgc
     1501 tcgcgcgcag gggaggaggg tgggggcgtc gtcgccgccc tggctgtggc gctctcgccg
     1561 ttcaccattg ccttcggcgg cacggcattt ctcgaccccc tgatggtgat gtggggactc
     1621 gcggcttgcg tcacggcgtc gcgcggccga ccagggtggg ccggcatctt cctcggcctg
     1681 tccttcgcga cgaaggtgca ggggctgcta ttcgctccat tggtcattct caccgcacag
     1741 ctcgcggaga gcgcagagag gtggaagaga cgcatctcca cgcgctctgc gtcctcggcg
     1801 gcaaaacagt gcctgagctc cgccagcggc tttggcattg gcgttgccct cgtcatcgcc
     1861 tggtcgctcc tgcgcagcgg gacgccgttc tgggagcagc aggcgatcaa ctacggcggc
     1921 ctccgattcg cgttcgccgc cgagctgggg ccgaggctga taagctggtc aggatttctg
     1981 ccgcatttgt tcgggccgat actgggaggt attctcgtcg tttgcctgat aagtttgctc
     2041 gtgcatgctt ttgggcatgg caaaggaacg agaggcgcgg cgatcgactt gctgctcgtc
     2101 gcatatatag tgggattcgt tgcgctgcac tggctgctgg cattcccggt gtgggatcgt
     2161 tacttgctga ttctggtgcc ggtcgcaggg gtgctgttgg atcgaagcgt ggctgtgacg
     2221 ctcgcctggt tatcttcgca tagccatgtt gctatcggga gaagcagtgc attggcagtg
     2281 gctgcaatcg gcacgatcgt gctgcccttc gcgctccagg ctgcgcatag cgagatcctg
     2341 atcggcggcg atcacggccc gcacgacggg attgatcgcg ccgccgcgta tctgcgcgaa
     2401 ttgcccgtgg gaaccgtcgt gtatgatcac tggctgagct gggagttcga ttactatttg
     2461 tgggacgcgc cgctttatcg cgcctacttc gacacgccgg ccgacctggc gcgcgatttg
     2521 cgcgtctttg gccggtcgtc gacgcgctac ctcgtcgtcc ctgcgaatga gtccctgggc
     2581 aagatcgagc gcgcggttgc cgatgcagga ttcaggatcg cgcccgtcat gaagtctgcc
     2641 gatcgcctcg gacgaactgc cttcgtcgtt taccgcacgg tccttcgtga gtgacttacc
     2701 cggtcgtgtg gcgaacttcc tgcctgctat gcaacgaccg caccatcggc gatcgccagg
     2761 ccctcgttga tgatctcgaa gacttccttc aactgcgcct cggtgatgca cagcggcggg
     2821 ttggtgaaga aagtgttgct gtgcgtgaaa atgtaaagcc ccttctgctt gaagaacgcg
     2881 gccagcttct gcatcggctc cgacgtgccg tcgaacggcg cgagcggctc cttcgtcttc
     2941 cggctcttca ccagctcgac gatcccgaac aggccgatcg agcgcacgtc gccgacggac
     3001 gggtgctcgg ccttcaagtc gtcgagcagg ccggccatca cgttgcccat gcgccgggcg
     3061 ttgcccacca ggtcgtcttc ttcaagcacc ttcagattgg cgacggccgc cgccagacac
     3121 atcggatgcc cgttgtaggt caatccaccg gagaacacac ggtcgtcgaa atagtgcgcg
     3181 atcttctcgc tcaacgcgac gcagccaagc ggcatgtacg ccgaggtcag ccccttcgcc
     3241 attgtgatga tgtcgggcac gacgttccag tgatcgacgg caaaccattc gcctgcccgg
     3301 cccagcccgc acatcacctc gtcgcagatc agcagaatcc cgtgccggtc gcagatctcc
     3361 cggagaccct gcaggtaacc atccggcggc acgatcaggc cgttggtgcc ggtgacggtc
     3421 tcgataaaga tggccgcgat cgtgtgcgcg ccttcgtaca tgatcacttc ctcgatctgg
     3481 tcgaggcatt tgcgggcaaa gacctgatcg ctgtcacccg gctgatagag cgggctgcgg
     3541 tatttgtatg gatcaaagac gcgcacgatg ccggggaggc ctggctcgtt ggcccagcgt
     3601 cgcgggtcgc cggtcaggct gatggcgccg gcggtggccc cgtggtaggc g
//
LOCUS       DBNHXB010000027         8592 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1012437, whole genome shotgun sequence.
ACCESSION   DBNHXB010000027 DBNHXB010000000
VERSION     DBNHXB010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8592)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1012437"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="AC162G_01080"
     CDS             complement(<1..616)
                     /locus_tag="AC162G_01080"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5074439.1"
                     /translation="MARVGFIGLGRMGVPMCRHILEAGHALTVFDHKPTAIEEMARAG
                     ASPAASPAEVGAASEVILVMVPTDDDVTAVVAGESGVLAGAAPGAVIAICSSAMPETC
                     VQIAAVAVQKGVGVVDAPVARGVRGAETGDLTVFAGGEAGDIEKCRPVFETFAKHIFH
                     MGPVGAGQITKTVNNLIHWVEVVGIYEALRLGAAHGVHPNKLRPA"
     gene            complement(633..2093)
                     /locus_tag="AC162G_01085"
     CDS             complement(633..2093)
                     /locus_tag="AC162G_01085"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR5074440.1"
                     /translation="MVATAIPKVEESSTTSGLRSWLEAVEACGELRRVTGASWQEDIG
                     MATEILQQTRDAPAVIFDEIPGHEPGTRVLVNNFGSMRRIALALDLPPDQDTRTLLDL
                     WRKKLRQVKPVPPVFVSDGLVLENVMRGDEVDVLKFPTPIWHEQDGGRYIGTASADIT
                     RDPDEGWVNLGTYRVMVHDKNRVGFYISPGKHGRLHRQKYFDRGEPCPVAVAIGMHPL
                     LYLASATESQYGESEYDWAGGILGHPVEVFKGEATGLPLPVDAEIILEGFAYPQESLV
                     EGPFGEWTGYYASASREEPVIHVERIYHRHDPIIVGSPPTRPPDEQARYRAFLRSAVM
                     FDQLEQAGVPDIQGVWCHEVGGSRLWVAVSIKQRYPGHARQVGHLAASVRAGGYLGRY
                     VIVVDEDIDPTNLEEVVWALCTRSDPERSIDLMKRAWSGPLDPAIRPGQKGFNSRAII
                     DATRPFEWRDEFPPVSAMSRERRQEAMKRWGYLLES"
     gene            complement(2120..2590)
                     /locus_tag="AC162G_01090"
     CDS             complement(2120..2590)
                     /locus_tag="AC162G_01090"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5074441.1"
                     /translation="MSKDELLVGEAGKAVIQKFRTRNTLEGLHHKNLKGMSLRIWADR
                     GGNSIWFDTYTRAMEAHLSEIPPGEHKRAHRHFHEALIYIVRGKGYSLIWADGEAPKK
                     YEWEEGDLLTIPVWHWHQHFNTDSQEVARYLAITNINHMDALGLFRHEEAPWPE"
     gene            complement(2693..3160)
                     /locus_tag="AC162G_01095"
     CDS             complement(2693..3160)
                     /locus_tag="AC162G_01095"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5074442.1"
                     /translation="MTQTRIVDGAAGREIITRFMHEHSHKGLFFKDLKNMPLDPWDER
                     GKNSRSTWFDTNTRMMEAHVSEIPPGTNKKAHRHFNEAIIYIVKGRGYSLIWAEGEEP
                     ARYDWEEGDMFAVPMNYWHQHFNRDPANTARYLAITNISFMESLGLFDTAQQE"
     gene            complement(3217..4461)
                     /locus_tag="AC162G_01100"
     CDS             complement(3217..4461)
                     /locus_tag="AC162G_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5074443.1"
                     /translation="MPETQTTSSASDALAGVRVVEFAVFAAGPVVGKHLAEHGAEVIR
                     VESRTRPDGFRVHYPPFKENRPGLERGGSFAIFNNDVLGVTLNLKSARGVELARALVA
                     RADVVIENFVPGVMGRLGLSYAALREVNPGLIMLSSCNQGQSGPRAAQRGFGSQLTSM
                     SGFTYLTGYGDGESPMLLYGPYIDFIAVGYGLIAVLAALDYRRRTGKGQHIDLSQYEC
                     GLQFVIPMLLDFQVNGQIQGRQGNRDPHAAPHGTYPCRGEDAWCVIAVFEEEEWRALC
                     AVTGHPEWAADPRFASLAARKAHEDELDRLVSMWTSEHTPHEVMEKLQAVGVQAGMVQ
                     SVGDLFACPQLAHRRQWRTLPHREFGAYAYEAPPFLLSETPAEIRRPSPLLGEHNRYV
                     FGELLGMPEAEIDKLLADGVIE"
     gene            complement(4479..5747)
                     /locus_tag="AC162G_01105"
     CDS             complement(4479..5747)
                     /locus_tag="AC162G_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5074444.1"
                     /translation="MTDEAGRTPGLLAPYRVLDLSDASGFLCGKILADLGADVIKVEP
                     PGGDPARWMEPFPTPQAQEKQGLYWLSYNTDKRGITLNLGASRGQELFGRLVRSADFV
                     IETYPPGRSVELGLGWETLRRLNPRLVLISITPFGQSGPYRDYKGSDLIAMAMSGMMS
                     LVGQPDQPPLRVSLPQAPLWAGMYAAAGGLIAHTHRELTGVGQQVDISLQSALLWALA
                     NAPAFWATNRTSLSRAGTVVTGRSITGATMRAIYPCQDGHINFIIYGGAAGRRSNEAL
                     VEWLAEEGLATGGLLKKDWDHFDIVTSTQTEIDELENPTQELFRRTTKAEFLEQGFKR
                     EMLGYPVANAHDILDDPHPQARGFWHTLDCPALGRTLKFPGGFARFSEASAGPCRPAP
                     RVGEHNAEIYSGELGLAEFELAELQRDGVI"
     gene            complement(5795..7384)
                     /locus_tag="AC162G_01110"
     CDS             complement(5795..7384)
                     /locus_tag="AC162G_01110"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR5074445.1"
                     /translation="MAVAVQTQMQETRAQVAKDIRSLRSTLDWLRAEDNLIETNVEVD
                     PDLEITGIQKLLDGSLPILFNNVKGYPHARALTNFFANMDILDRMFGWKDRKDRTHKL
                     AYALTHPIPPVEVPQDEAPCQEEVITDDLDVNKYVTAIRHTELESERTIGSGNSVVVG
                     PYFHGGSHIGYNRMNARWGNVCTFQSAPGSHMWQVITEHYHDDKPIPLTMCFGVPPAA
                     TLIAGGGYDYVVLPRGGDELGAAGAVQGFPIRIVKARTVDAWALADCELVLEGHLYPR
                     DKRYETAEAEAAGVQGKLPFHPEWAGYMGKAYRAPTFHVTGITLRRRETKPIFYVLGV
                     HMLDCNNIDTTVREAAIFELCNRIQPDLVQDVNIPFCMTDWAGCIIQVKKRLKTDDGW
                     VRNFLVATLACSAGMRLAIAVDSDVDIYNMDEIIWCLTTRVNPRTDILNPVPGGAGQT
                     FIPEERLTAGDRQWTAMNTRFEGGMAIDTTVPYGYESDFQRPVYPTGRVELDKWFKPD
                     EIAHAKSLMKGWVELLARTGR"
     gene            complement(7415..8143)
                     /locus_tag="AC162G_01115"
     CDS             complement(7415..8143)
                     /locus_tag="AC162G_01115"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR5074446.1"
                     /translation="MFRKVQAVRASKDVVAQVEQAIFSGRLSPGDRLPSERELAEQFG
                     LSRITVRDALRILESNGLIEIKVGASGGAFVREPNFDPLRNSLSSMLKFKKASILELA
                     EARKVIETATAELATQRATPEDLQALRSAVEAARQSLDTGDLYYMPHSVSFHIALAQA
                     AKNSVLYLTVNSFRTLFFNVLEQLLPTVDMAERAIEDHWAILQAIEAGDGARARQLMM
                     EHLTYFENKVRALQDGPLFEREES"
     gene            complement(8297..>8592)
                     /locus_tag="AC162G_01120"
     CDS             complement(8297..>8592)
                     /locus_tag="AC162G_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014671.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIR5074447.1"
                     /translation="HNTVDRLWGKAMKQNVPTEGLVLLATGRISSEMLGKAAKMGVPV
                     VASRTSATSLSVALGRAWNISVIGYVRRNSLRVYAAPQRIQSAREPARVTRAG"
BASE COUNT         1557 a         2720 c         2568 g         1747 t
ORIGIN      
        1 gtgccgggcg caacttgtta ggatggacgc catgcgccgc gcccaggcgc agcgcctcgt
       61 agatgcccac cacttcaacc caatggatca agttgttgac cgtcttggtg atctgcccgg
      121 cgccaactgg ccccatatgg aagatgtgtt tggcaaaggt ctcaaaaacc gggcggcact
      181 tttcgatatc accggcttcc ccaccggcga ataccgtcag gtcgccggtc tcagcgccgc
      241 gcacccctcg cgccaccggc gcgtctacca ccccaacgcc tttctgtacg gcgacggcgg
      301 cgatctggac gcacgtctcc ggcatggctg aactgcaaat ggcgatcacc gcccccggcg
      361 cagcgccagc tagcacgccg gactcgccgg ccaccactgc cgtcacgtca tcgtcggtgg
      421 gcaccatcac cagaatgact tcgctggccg cgcccacctc cgctggcgac gcggcaggtg
      481 aagcacctgc cctggccatc tcttcaatcg ccgttggctt gtgatcaaag acggtcagcg
      541 cgtgaccggc ctccaaaata tgccggcaca tgggcacccc catgcgtccc agaccgatga
      601 aacctactcg ggccacgatt atcccttcct tgttaagact ccaataaata tccccagcgt
      661 ttcatggcct cttgtcgccg ctctcggctc atcgcgctca ccggcggaaa ttcgtcgcgc
      721 cactcgaagg gtcgcgtcgc gtcgatgatc gctcgcgagt tgaagccttt ctggccgggg
      781 cggatcgccg gatcgagcgg cccgctccag gcgcgcttca tcaggtcgat cgagcgttca
      841 ggatcggagc gggtgcacaa ggcccagacc acctcttcaa ggttggtcgg gtcgatgtct
      901 tcatctacca cgatcacata gcgccccagg tagcctccgg cccgtaccga ggcggccaga
      961 tgccctacct gtcgcgcatg gcccggataa cgctgtttga tgctgacggc cacccacaac
     1021 cggctacccc ccacttcgtg gcaccacacc ccctgaatat cgggcacgcc ggcctgctcc
     1081 agttggtcaa acatgacggc cgagcgcaaa aaagcccggt agcgcgcctg ctcgtccggt
     1141 gggcgagtcg ggggcgagcc gacgatgatc ggatcgtggc gatgatagat acgctcgaca
     1201 tgaatcaccg gttcttcgcg gctggcgctg gcgtagtagc cggtccattc tccgaacggc
     1261 ccctcgacca ggctctcctg tggatacgcg aacccttcca ggatgatctc ggcatccacc
     1321 ggaagcggta ggccggtggc ttctcctttg aacacctcaa ccgggtgtcc caggatgcca
     1381 ccggcccagt cgtactcgct ctcgccgtac tgcgactcgg ttgcgctggc caggtagagc
     1441 agcggatgca tgcctatggc cacggccacc ggacagggtt cgccgcggtc gaaatatttc
     1501 tgccggtgca ggcgaccgtg cttgccgggg gagatgtaga accccacccg attcttgtcg
     1561 tgcaccatca cacggtaggt gcccaggttc acccagccct catccggatc gcgggtgatg
     1621 tcggcgctgg cggtgccgat gtagcggccg ccgtcctgct cgtgccagat cggggtggga
     1681 aacttcagga catcgacttc gtccccacgc atcacattct ccagcaccag gccgtcgctg
     1741 acgaacaccg gcggcacagg cttcacctgg cgcagtttct tccgccagag gtccagcagg
     1801 gtcctcgtgt cctggtccgg cggcaagtca agcgccaggg cgatgcgccg catcgagccg
     1861 aaattgttga ccaggactcg agtgccaggc tcgtgtccgg gaatctcgtc aaagattacg
     1921 gccggcgcat cccgggtttg ctgcaaaatc tccgtcgcca tgccgatgtc ttcctgccag
     1981 gaagcgccgg tgacccggcg caattcgcca caggcttcga cagcctccag ccagcttctc
     2041 agccccgagg tcgtcgatga ctcttcgact ttcggtatcg ccgtagcgac catgttcgct
     2101 cctcaatgac cgatgagctc tactccggcc acggcgcctc ctcgtgtcta aaaagcccca
     2161 aggcgtccat gtggttgatg ttcgtgatcg ccaggtagcg cgccacctct tgcgaatcgg
     2221 tgttgaaatg ctggtgccag tgccagacgg ggatggtgag caggtcgccc tcttcccact
     2281 cgtatttctt cggcgcctcg ccgtcggccc agatgagcga gtaccccttg ccgcgcacga
     2341 tgtagatcag cgcctcgtga aaatggcgat gcgcgcgctt atgctccccc ggcggaatct
     2401 ccgacagatg ggcttccatc gcccgcgtgt aggtgtcgaa ccagatcgag ttgccacctc
     2461 gatcagccca gatgcgcagg ctcatccctt tcaggttctt atggtgcagg ccctcaaggg
     2521 tattccgggt ccggaacttc tggatgacgg ccttacccgc ctcgccgacc aacaactcgt
     2581 ctttggacac actgcctcct ttagatccga aaaccggagt gcaggcttca gcctgcatgg
     2641 ccaaagcggg ctgaagcccg cactccgagg ttaaacagat gcccttgctg aactactcct
     2701 gttgcgccgt gtcgaacagc cccaggctct ccatgaaact gatgttggtg atcgccaggt
     2761 agcgcgccgt attggcggga tcgcggttga aatgttggtg ccagtagttc atcggcacgg
     2821 cgaacatgtc gccttcttcc cagtcgtagc gcgcaggctc ttcgccctcc gcccagatga
     2881 gcgaatagcc tctgcccttg acgatgtaga tgatcgcctc gttgaagtgg cgatgcgcct
     2941 tcttgttcgt gcccggtgga atctcggaga cgtgcgcttc catcatgcgg gtgttcgtgt
     3001 cgaaccaagt cgagcggctg ttcttgccgc gctcgtccca gggatcgagc ggcatgttct
     3061 tcaggtcttt gaagaacagg cccttgtgcg aatgctcgtg catgaagcgg gtgatgattt
     3121 cgcgcccggc ggcgccatca acgatgcgtg tctgtgtcat ggggttcgcc tccttttgaa
     3181 gtttgaactt tgaagtttga attttgcatc gatgccttat tcgatcacgc cgtcggcgag
     3241 cagcttgtcg atttcagcct ccggcatgcc gagcaattct ccaaagacgt atcgattgtg
     3301 ttctcctaag aggggactcg ggcgacgaat ttcggcgggt gtttccgaaa gcaagaaggg
     3361 cggggcctcg taggcatatg cgccgaattc gcggtgcggc agcgttcgcc actgtcttcg
     3421 gtgcgccaac tgtgggcaag cgaacaagtc gccgacactc tgaaccatgc cggcctggac
     3481 cccaacggcc tgtagtttct ccatgacctc gtgtggtgtg tgttcggagg tccacatgct
     3541 caccaggcga tccagctcgt cttcgtgcgc tttgcgcgct gccagagagg caaagcgcgg
     3601 atcggcagcc cattctggat gtccggtcac tgcgcagagc gcgcgccatt cctcttcctc
     3661 gaagacggcg atgacgcacc aggcatcttc gccccggcac gggtaggtgc cgtgcggcgc
     3721 ggcgtgcgga tcgcggttcc cctgccgccc ctgaatctgc ccgttgacct ggaagtcgag
     3781 cagcatcggg atgacgaatt gcaggccgca ctcgtactga gagagatcga tgtgttggcc
     3841 tttgccggtt cggcggcgat aatccagcgc cgccagcacg gcgataagcc catacccgac
     3901 cgcgatgaag tcaatgtaag gaccatacag cagcatcggc gattcgccgt cgccatagcc
     3961 ggtcaggtac gtaaagccgc tcatcgaagt aagctgcgag ccaaagcccc gctgcgcggc
     4021 gcgagggccg gactgtccct ggttgcacga actgagcatg atcaatccgg ggttgacttc
     4081 tcgaagggcc gcgtaactca ggccgaggcg ccccatgacc cctggcacga agttctcaat
     4141 gaccacgtcc gcacgggcga cgagcgctct ggccagctcc acgccacgcg cgctcttcag
     4201 attgagcgtc acgccgagca catcgttgtt gaatatggcg aacgagcccc cgcgctcaag
     4261 gccaggacga ttttccttga agggcgggta gtggactcgg aacccatccg ggcgagtgcg
     4321 cgactcgacc cggatgacct ccgcaccgtg ttcggccagg tgcttcccca cgaccgggcc
     4381 ggcggcaaac actgcgaact cgacgacgcg caccccagct aatgcgtcgg acgccgaaga
     4441 tgtcgtttgt gtctctggca tggcgagtgg tgacaggtct aaatcactcc gtccctttgc
     4501 agttcggcca gctcgaattc ggccaggccc agttcacccg aatagatctc ggcgttgtgc
     4561 tccccgactc tcggcgcggg ccggcacggg ccggcgctgg cctccgagaa acgcgcaaag
     4621 ccgcctggaa acttcagcgt tcggccgagc gccgggcagt cgagggtatg ccaaaaacct
     4681 cgggcctgtg gatggggatc gtccaggata tcgtgcgcgt tcgccaccgg gtaaccgagc
     4741 atctcgcgtt tgaatccctg ctccaggaat tccgccttcg tggtgcgccg aaacaattcc
     4801 tgggtgggat tctcaagctc atcgatctct gtctgagtgg atgtcacgat gtcaaagtga
     4861 tcccagtcct ttttcaacaa gccgccggtc gccagaccct cctccgccag ccattccacc
     4921 agggcctcgt tcgagcgccg gccggctgcg ccgccgtaaa tgatgaagtt gatgtggcca
     4981 tcctggcaag gatagatcgc ccgcatggtt gccccggtga tgcttcgacc tgtcactacg
     5041 gtaccggcgc gactgaggct tgttcggttt gtcgcccaaa aggccggggc gttagccaag
     5101 gcccacaaca aggcgctttg aagcgaaatg tccacctgct ggccgacgcc cgtgagttcg
     5161 cgatgggtgt gggcgatcaa accccctgcg gcagcgtaca tgcctgccca tagcggcgct
     5221 tgtggaagcg agacccgcaa tggtggctgg tccggctgtc cgacgagtga catcatcccg
     5281 ctcatcgcca ttgcgatcag atcgctccct ttgtaatctc tatatgggcc gctctggccg
     5341 aagggtgtga tcgagatcaa caccaggcgt gggttaagcc gacgcagcgt ctcccaaccc
     5401 agcccaagct caaccgaccg gcccggtgga tacgtttcga tcacgaagtc ggcggaacgg
     5461 accaatcgtc cgaaaagttc ctgcccccga gaggcaccca ggttgagtgt gattccccgc
     5521 ttgtccgtgt tgtaactcag ccaatagagg ccttgtttct cctgcgcctg gggggtcgga
     5581 aacggctcca tccagcgcgc cgggtcaccg ccgggtggct cgaccttgat cacgtctgcc
     5641 cccaggtcgg ccaggatttt cccgcacaga aagccggagg catcggacaa atccagcacg
     5701 cgataggggg ccaacagacc gggtgtgcgt cctgcctcgt cagtcattga cttcacgtcc
     5761 ctaaactgag cagagcgata cgggctttcc ccggctaacg gcccgtgcgg gccagcaatt
     5821 cgacccagcc cttcatcaat gacttagcgt gcgcgatttc atctggcttg aaccacttgt
     5881 ccagctcgac gcgtccggtg gggtacaccg gtcgctgaaa gtctgactcg tagccatagg
     5941 gcacggtggt gtcaatcgcc atgccgcctt cgaagcgcgt gttcatcgcc gtccactggc
     6001 ggtccccggc tgtcagtcgc tcctcgggaa tgaaggtctg cccggctccg cctgggaccg
     6061 ggttgaggat gtccgtccgc gggttcacgc gcgtcgtgag acaccagata atctcgtcca
     6121 tgttatagat gtccacgtcg ctgtccacag cgatcgcgag gcgcatgccc gcgctgcacg
     6181 cgagcgtggc gacgaggaag ttgcgcaccc agccatcgtc tgtcttgagc cgcttcttca
     6241 cctggatgat gcagccggcc caatccgtca tgcagaatgg gatgttcacg tcctggacaa
     6301 ggtccggctg aatccggttg cagagttcga aaatggcggc ctcgcgcacc gtcgtgtcga
     6361 tgttattgca atcgagcatg tgcacgccga gcacgtagaa gatgggtttg gtctcacgcc
     6421 ggcgcagggt gatgccggtg acgtgaaagg tcggcgcccg gtaggccttg cccatgtagc
     6481 cggcccactc cgggtggaag ggcagcttgc cctgcactcc ggcggcctcg gcctcggccg
     6541 tctcgtatcg cttgtcgcgt gggtacaggt gcccttccag cacgagctcg caatcggcca
     6601 gcgcccacgc atccaccgtg cgcgctttga cgatgcggat ggggaagccc tgtacggccc
     6661 cggccgcacc caactcgtct ccaccacgcg gcaagaccac gtaatcgtat ccaccgccgg
     6721 cgatgagggt cgcggctggg ggcacgccga aacacatggt caatgggatc ggcttatcgt
     6781 cgtggtagtg ttccgtgatg acctgccaca tgtgagagcc gggcgccgac tggaaggtgc
     6841 acacattgcc ccagcgggcg ttcatgcggt tgtagccgat gtggctgccg ccatgaaaat
     6901 acgggccgac gaccacgctg ttgccgctgc cgatggtgcg ctctgattcg agctcggtgt
     6961 ggcggatcgc cgtcacatac ttgttgacat ccagatcatc tgtaatgact tcttcttggc
     7021 agggcgcttc gtcttggggt acttcgaccg gcgggatggg atgcgtgagc gcataggcga
     7081 gtttatgggt gcggtccttg cggtccttcc agccgaacat gcgatccaga atatccatgt
     7141 tggcgaagaa attggtgagc gcacgcgcgt gaggatagcc cttcacgttg ttgaacagaa
     7201 tgggcagact cccatccaac agtttctgaa tgccggtgat ctcgaggtcc gggtctactt
     7261 ccacattcgt ctcgatcaaa ttgtcctcgg cccggagcca atccagcgtg ctgcgaaggc
     7321 tacggatgtc ctttgcgacc tgcgcgcgcg tctcctgcat ctgggtctgc actgcgactg
     7381 ccatagattt cctccttgtg tcgggtcaca tccttcacga ctcttcccgt tcgaacagtg
     7441 ggccgtcttg cagcgcccga accttgttct cgaagtaggt caaatgctcc atcatcaact
     7501 ggcgcgctcg cgcgccatcg ccggcttcga tcgcctgcag gatggcccag tggtcctcga
     7561 tggcccgttc cgccatgtcg acggtgggca agagttgttc aagcacattg aagaacaggg
     7621 tgcgaaacga gttgacggtc agatacaaga cgctgttctt ggccgcctgg gccagagcga
     7681 tgtgaaagct cacgctgtgt ggcatgtagt acagatcgcc tgtatcgagc gactgcctag
     7741 cggcctcgac ggcgctgcga agcgcttgca gatcttcggg ggtggctcgc tgagtagcca
     7801 gctcagcggt tgcggtttcg atcactttcc gcgcctcggc cagttccaga atcgaggctt
     7861 tcttgaactt cagcatggag gaaagcgaat tgcggagcgg atcgaaattc ggttcacgga
     7921 cgaaggcccc tccgcttgcg ccgactttga tctcgatcag gccattcgac tcaaggatgc
     7981 gcaaggcatc gcgaaccgtt attcggctca gcccgaattg ttcggccagt tcgcgctcgg
     8041 aaggtaagcg atcgccgggc gacaatcgcc cggaaaagat cgcttgctct acctgcgcga
     8101 caacatcttt cgaggcccgg accgcctgaa ctttgcgaaa cataaccaat ccgtgattaa
     8161 gaatataatg ttaaatggta tgatggtaat accattatac atagaagtat ttcattgtca
     8221 agaagagatt cccacaatgt gtaagcgtgt cttgcctgat gtagatgatc ggctgttctt
     8281 ggggagatgc ccatgttcac ccggcgcgtg tcacgcgcgc cggttctctg gcagactgga
     8341 tgcgctgtgg ggcggcgtac acgcgcaggc tgttgcggcg cacgtagccg atcaccgaga
     8401 tgttccaggc ccggcccagc gcgaccgaca ggctggtcgc cgacgtgcgc gaggcgacga
     8461 cgggtactcc catcttcgcc gccttgccga gcatctccga gctgatccgc ccggtcgcca
     8521 gcagcaccag gccctcggtg ggcacgttct gcttcatggc cttgccccac aaccggtcga
     8581 ccgtgttgtg gc
//
LOCUS       DBNHXB010000028         3190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3864631, whole genome shotgun sequence.
ACCESSION   DBNHXB010000028 DBNHXB010000000
VERSION     DBNHXB010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3864631"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="AC162G_01125"
     CDS             <1..1418
                     /locus_tag="AC162G_01125"
                     /inference="COORDINATES: protein motif:HMM:NF022481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein O-mannosyl-transferase family"
                     /protein_id="HIR5074448.1"
                     /translation="WGGGDFATFQTKAFTRQIESNVFGHPMWVVLAQPFVALPVGDVA
                     FRANLVSAVFASAALAFVFLAAQRITRSMGASLLATGVLMVSHTFWTYAVMPKPYSLN
                     ALMLAMCVYLVVRWGQEKRGRDLYLFAIVYGLSPLNHLVMFTTAAGFLVFIVLVARRA
                     SEARRQTAIAGVIYLAALLPYMLLVTSAGEGEGTGATIGAFLRGFATALTSPLALALG
                     LAAGATLLVYQFMFSLPLGLLGLARSWQTDRSIAALLLLIVLGNVAFLLGATDPRTGG
                     EYVWNLHYYLMTYTVFSLWIAIGFAHLWPRLTRTRLRQAASLALFMAVPVTVYAVAPA
                     IARPLATNLPGFRELGGRDNLTYVLSPWKHNETGARPFAESVLAALPPHSTLFADYSI
                     WAIVNYLQVVEGVRPDVIVHKLPGTGSTQQLPFILERADTGHLYLADVNRYYDLADIQ
                     TRFDVTPAGPIYRLVPTQAPD"
     gene            1418..2557
                     /locus_tag="AC162G_01130"
     CDS             1418..2557
                     /locus_tag="AC162G_01130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5074449.1"
                     /translation="MNILHIYKDYFPVLGGIENHIRVLAEASASLGHDVTVLVTSLDR
                     RTRIETSNGVKLVKTGRWINVSSAPISPAMFAWARKLGRRADIVHLHFPYPLGETAHL
                     FSGSRARTVITYHSDIVRQKNLLRLYRPLLWRVLRKADRIITTSPNYIDTSPFLSHVR
                     DKCMVIPPGADVERFASVDSARTSALRGQLTHHEAAPILLSVGRLRYYKGLDTLLQAL
                     PALPTARCVIVGSGPMEAEWRALAASLDVADRAIFAGDVPDADLPLYYHAADLYVLPA
                     NARAEAFGVALVEAMAAGLPCVTTEVGSGTSWVVQDGVTGLVVPPRDPGALSVAIAGL
                     LDDEEKRATMGRAGRARARAEFSVDRMIDRVFSAYASLLEGIAAD"
     gene            2671..>3190
                     /locus_tag="AC162G_01135"
     CDS             2671..>3190
                     /locus_tag="AC162G_01135"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5074450.1"
                     /translation="MRVLIMGGAGFIGSHLAERALRDGWQVRVLDNLSTGRRENLAGL
                     EVDFVEGDLCDGRLVREVTQGCDLVCHLAAVVGVPVVLKDVLGALRSNVRGTEVVLEA
                     AAALGVRVCLVSSSEVYGRSDRLPLREDDASLLGPTRVPRWSYAAAKIVDEYLALAYH
                     AQRGLPVSIVRYF"
BASE COUNT          497 a         1046 c          996 g          651 t
ORIGIN      
        1 tgtggggcgg cggtgacttc gccacgtttc agacgaaagc cttcacccgc cagatcgaaa
       61 gcaacgtctt cggtcatcca atgtgggtcg tcctggcgca gccatttgtc gcgctaccgg
      121 tcggcgacgt cgcctttcgc gccaatctcg tctcggccgt gttcgcttcg gcggcgcttg
      181 cattcgtttt ccttgccgcg cagcgcatca cccgctcgat gggcgcttcg ctgctggcga
      241 ccggtgtgct catggtctct cacacgttct ggacgtatgc cgtgatgccg aagccctact
      301 cgctgaacgc gctcatgctc gccatgtgtg tctatctcgt cgttcgttgg ggacaagaga
      361 agcgcggtcg tgacctgtac ctgttcgcga tcgtttatgg gcttagccca ctgaatcatc
      421 ttgtcatgtt caccaccgca gccggattcc tggtgttcat tgtgctcgtc gcgcgccggg
      481 cctcggaggc gcgccggcag accgccatcg cgggcgtgat ctacctggcg gccctgctgc
      541 cgtacatgct actggtcacg agcgcgggcg aaggcgaggg cacgggcgcg acgattggcg
      601 cgtttctgcg cggcttcgcc actgcgctga cctcacccct tgccctggcg ttgggactcg
      661 ccgccggcgc gacgctgctg gtctaccagt tcatgttcag tctgccgctc gggcttttgg
      721 gtctggcacg atcctggcag accgaccgat ccatcgccgc actgctgctg ctgatcgtgc
      781 tgggaaacgt cgcctttctg ctcggcgcga ccgatccgcg cacgggcggc gagtacgtct
      841 ggaatctgca ctactatctc atgacctaca ccgtcttcag cttgtggatc gcgattgggt
      901 tcgctcacct gtggccgcgc ctgacgcgaa cccggttgag gcaggcggcg agtctcgcgt
      961 tgttcatggc cgtgcccgtg accgtgtatg ccgtcgcgcc ggcgatcgcg cgtccactcg
     1021 cgacgaacct gcccggtttt cgcgagctgg gtggccgcga caatctcacc tatgtgctgt
     1081 cgccgtggaa gcacaacgaa acaggtgccc ggccgttcgc cgaaagcgtc ctcgccgcat
     1141 tgccgccgca cagcacgctg ttcgccgatt acagcatctg ggccatcgtc aactatctac
     1201 aggtcgtgga gggcgtgcgg ccggacgtga tcgtacacaa gctgcccggc acaggctcaa
     1261 ctcagcagtt gccgttcatc ctggagcgcg cggatacggg ccatctttat ctcgccgacg
     1321 tgaatcgcta ttatgatctg gccgatatcc agactcgctt cgacgtcacg ccagccgggc
     1381 cgatttatcg gttggttcca acacaggcgc cggactgatg aacatcctgc acatctacaa
     1441 agactacttc cccgttctgg gcggaattga aaaccacatt cgcgtgctcg ccgaagcgag
     1501 cgcctcactc ggccacgacg tgacggtgct ggtcaccagc ctcgaccgac gcacgcgcat
     1561 cgagacgtcg aacggcgtga aactggtgaa gaccgggcgc tggatcaacg tgtcttctgc
     1621 gccgatcagc ccggcgatgt tcgcctgggc gcggaagctg ggccgccgcg ccgacatcgt
     1681 tcatctacac ttcccctacc ctctcggcga aaccgcgcac ctcttcagcg gatcgcgcgc
     1741 aaggacggtc atcacctatc acagcgacat cgtgcgccag aaaaacctgc tgcgcctcta
     1801 tcgtccgctg ctgtggcgcg tcctgcgcaa agccgaccgc atcatcacca ccagcccgaa
     1861 ctacatcgac acatcgccgt tcctcagcca tgtccgagac aaatgcatgg tcatcccgcc
     1921 gggcgccgat gtcgagcgat tcgcatccgt cgattccgcg cggacctctg cgctccgcgg
     1981 ccagttgacc catcacgaag ccgcgccgat cctgttgagc gtcggccggc ttcgctatta
     2041 caagggcctc gacaccttgc tgcaagcgct gcccgcgctt cccaccgcac gatgcgtgat
     2101 cgtgggatca gggccgatgg aggccgagtg gcgtgcgctg gctgcgtcgc ttgacgtggc
     2161 ggatcgcgcg atctttgccg gcgacgtgcc cgatgcggac ttgcccctct attatcacgc
     2221 cgccgatctc tacgtgttac ccgcgaacgc gcgcgccgag gcgttcggcg tcgcgctcgt
     2281 cgaggcaatg gcagccgggt tgccgtgtgt cacgaccgag gtcggctccg gcacgtcgtg
     2341 ggtcgtgcag gatggcgtga ccggcctcgt cgtcccaccg cgcgatccgg gtgcattgtc
     2401 ggttgcgatc gcaggcttgt tggacgacga ggaaaagcgt gcgacgatgg gccgggctgg
     2461 gcgggcgcgg gcgcgggccg aattcagcgt cgatcggatg atcgaccgcg tattctccgc
     2521 ttatgcgtca ttgctcgaag gaattgccgc tgactgaaca ccggtcactg ttcactgatt
     2581 actgactact gatcactgat cactgattac cgatcactga caagtgactc gtcaccaccc
     2641 ttttcaccgc ctaaggtata attggtttct atgcgcgtgt tgatcatggg cggcgcaggc
     2701 ttcatcggct cgcacctggc cgagcgcgcg ctgcgcgacg gctggcaggt gcgggtgctg
     2761 gacaatctca gcacgggacg acgcgaaaac cttgccggct tggaggtcga tttcgtcgag
     2821 ggcgacctct gcgacgggcg actcgtgcgc gaggtcacgc agggctgtga cctggtatgc
     2881 cacctggccg cagtcgtcgg cgtacccgtg gtcttgaagg acgtactcgg cgcgctgcgc
     2941 tccaacgtgc gcggcacgga agtggtgctg gaagcagccg ccgcgctcgg cgtgcgcgtg
     3001 tgcttggtct cgtcatccga ggtgtatggg cggagtgacc ggctgccact gcgcgaggac
     3061 gacgccagcc tgctcggccc gacccgggtt ccgcgctggt cgtacgccgc agccaagatc
     3121 gtcgacgagt atctggcgct ggcttatcac gcgcagcgcg ggctgccggt ctcgatcgtg
     3181 cgctatttta
//
LOCUS       DBNHXB010000029         8847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3361676, whole genome shotgun sequence.
ACCESSION   DBNHXB010000029 DBNHXB010000000
VERSION     DBNHXB010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8847
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3361676"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="AC162G_01140"
     CDS             <1..414
                     /locus_tag="AC162G_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR5074451.1"
                     /translation="ELGADEVVDYTATDAVEAVRGLTGGRGVDCAFECAGTPAAMVTA
                     VNCVRRGGRVALVGLTGNKPVELNLDRLVLDEIDVFGVRSSPNAYPELIQLIAAGKVN
                     VRKLVSRVYPLEQINDAFEAFRQREGGAIRMVIKI"
     gene            411..2126
                     /locus_tag="AC162G_01145"
     CDS             411..2126
                     /locus_tag="AC162G_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR5074452.1"
                     /translation="MSRHAGDVLAEMLIGYGATAVFGLAGGQTLPLYDAIRSRAGDIR
                     HVPLRDERSAAYAADGYARLSGRVGVCDATVGPGAIKFTSGLAEALNSSIPMVALVSD
                     MPSDWLAVRYRGGGNQLVDQLAVLAPLCKWTGRLPNPGKMPELVQRAFQMATTGRPGP
                     VVIELPEDLFKASFEAATPAVDARFGAAPAFRPVPDPDSVRAALALLEPAARPVMIAG
                     GGAMLSGAGRSVQALAERLGLPVATTLSGKGIIPETHPLALGVLGALGGGAAARKAVE
                     EADVIFAVGFKFGQNPTYRWTLPKRGQRIVHLDIDAAELGKMFPVEVGMVADARAGLD
                     AMLAASTAERPMDAVASKVAALRAEWQAQLAADATQARPIKPQQVGMALDEVAGPDSV
                     LVCDASFASGWGGMYFTVRNERRAIFPRGMAGLGWGLPAAIGAQIARPKSKVIVLAGD
                     GAMTYCLGELATLVQEGLNVTVVVLNNSAMGWIKWEQAVFWDGKFQSTDLSQVDFAMV
                     ARGLGCLGMNVSEPGDLNEALAQALAADAPAVIDVRTAAGEAAVPKFTESAQARALMQ
                     DKLAG"
     gene            2161..3222
                     /locus_tag="AC162G_01150"
     CDS             2161..3222
                     /locus_tag="AC162G_01150"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HIR5074453.1"
                     /translation="MREYRIAVIPGDGIGKPVIAEGLKVLQRAAAVQGGFQLKPRHFD
                     WGCEYYLAHGRMMPADALEQLAGFDAIYLGAVGYPSVPDHISLGGLLLPIRRGFDQYV
                     NLRPIQLLPGVQSPLRTAEPIDFVCVRENTEGEYAGIGGRFCAGTEHEVVTQTALFSR
                     RGTERVIRYAFELARQRPRRKLTSATKSNALQYSMVFWDEVFAEVAADYPDVETEKWH
                     VDALAARFVTCPQTFEVVVASNLFGDILTDLGGALQGSLGLPASANINPTRQYPAMFE
                     PVHGSAPDIDGKGIANPIAAIWAGAMMLDVLGETQAARLVMDGVRATTAAGRTLTPDL
                     GGGAKTVEVGDEICRQISL"
     gene            3315..4796
                     /locus_tag="AC162G_01155"
     CDS             3315..4796
                     /locus_tag="AC162G_01155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5074454.1"
                     /translation="MDQAHRTTALSAKIGLSAREQWLLATLMLPSAMELIDSGMLAVA
                     LPRIQADFGVPVDVLSWAVAAGFLPRVTLMPVYGHIGDLFGKKRLFVLGLTVFLVGAT
                     LGIFAPSFSWLVVGRLLQGLGSSSLPLAMALIAAEFPYDRRGRALGIWNTAAPVGIIL
                     GPVLGGFIINAFGWHAMFVFVSLGSALALAATAWLLPETTSASARSGFDWVGAVSLML
                     VILGLLLATTTESLVPIGSPVNLAFWGISALAIVSLLRNARRKRDAFVGLDILRNRRF
                     MSPALAVSLRMFAHDGARFLLALILANVFRQSPRSVGVQMLFYAVPLMIGVATGGYLA
                     DRWASRVVGVVGMIGQVAGLLWLAVADASLGAAALVPGMIVAGLASGMNLVALTKEAI
                     GALGERRVGLASGLYNTIRFAGTAAATPLLGLLLASGFAQQGGDQTVSGPYQLAFGVA
                     AGVAALGAVAAALIPAPAPTPTLPRAAGEGAKAPVSTRGGPSL"
     gene            4793..5890
                     /locus_tag="AC162G_01160"
     CDS             4793..5890
                     /locus_tag="AC162G_01160"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5074455.1"
                     /translation="MIDDSKIGEALLHWTPRFLANGVDYNELQRIIARMRKWDDWCRA
                     WSEIGAEHEAQAIVALAAGHAITAAGAFTRAAIYYHFGQMIFYTDTAQKNAAHLKKVE
                     CYARAAPLLRPPAVRLEIPYEDITLPAYVRLPEGGGPHPCVILVCGLDSVKEQESHWE
                     EPLLSRGMATLSFDGPGQGETWFRMKMRLDYETAVTAVIDTLSAWPEIDVARVGLLGH
                     SMGGHFGARAAARDPRVAACALIAGFFTLRRWEEMSVFLRAGLKHVFGVGSEAEARER
                     AAQLTLEGFAGDIRCPLLIVHGAKDTLLPVEEAHRLKAAVSGPADLLIFPEGNHSCNN
                     MPFRVEPAVADWLADRLVAGPSRVGRREARI"
     gene            5926..6336
                     /locus_tag="AC162G_01165"
     CDS             5926..6336
                     /locus_tag="AC162G_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5074456.1"
                     /translation="MKLKKCSVDDVEARPAPNRHIRELFSGQTLGAQGVTFRVVDMLP
                     ASRQEPRRPHSHPDFEEVIFILSGQGRIWAEGEWLEVQAGDAVLVPPGVVHATFNTTE
                     QTLRLASFFPVAEGVDQRTRAAQYVTLDLEPGNA"
     gene            6370..7602
                     /locus_tag="AC162G_01170"
     CDS             6370..7602
                     /locus_tag="AC162G_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase"
                     /protein_id="HIR5074457.1"
                     /translation="MATRSLLSKLYVKVLAPDRPPYQVRDEEYHRFDQRNNMTVGRPN
                     WDESIKAFTGKTETTRVRRIQSGRPGYAVQDYSLFLASGVVAYRLGTGINHANRGLTA
                     WASLGTNLPKGVDRWQGTPEAATAMVKRAAHFLGADRVGVAPLDRRWIFSHAFWSDGS
                     HKEIVFEDVEAPVETDKQMIIPNRMQWVIVMGTQMDPEMMKYTPTPVGCAETRVTYSR
                     LALQVAGLAEFLRGVGYQAIPSINDLGLNIPMAIDAGFGEQGRHGKLVTPEFGPSVRL
                     CKVITDLPLVRDYPIRFGVTEFCEVCQKCAEACPSNAISTGGRAWSGPNLSNNPGVFT
                     WHLDNESCRRYWELGPADNCTACIRACPFTKGRSRAHDLTRTVISNAPRLNRLWRKLD
                     DVLGYGAEQDATHFWEGK"
     gene            7841..>8847
                     /locus_tag="AC162G_01175"
     CDS             7841..>8847
                     /locus_tag="AC162G_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR5074458.1"
                     /translation="MKALVIEEFGGPMIPKEMPVPDPGPGEVLLRVKACAVDQFDLRI
                     RDGKVPHARVPLILGHEVAGEVAACGQGVTEWTAGQRVTSTLYLTCGRCRKCQIGRET
                     ICENFQGYLGIQTPGGYAEYTLVPAVNLVALPDSISFADASVIANAIGTPYHALTKRM
                     RLQPGERVIITGAGGGVGVHAIQLARMMGALVMAVDIGSAKLEAAREYGADIAADPTQ
                     GDFAGMAREWTKGEGVEAVLELVGAATIPTSLRALGKGGRLVIVGHHGGSEFTLQASQ
                     VLANEWEILGSRNVSKRELAEVVSLVAAGRVRPIVTGLHPLEEAESVHARLRQQQIVG
                     R"
BASE COUNT         1449 a         2660 c         3141 g         1597 t
ORIGIN      
        1 gaactcggcg cggacgaagt cgtcgattac accgcaacag acgccgtcga ggccgtacgt
       61 ggcttgacag gcgggcgcgg cgtggactgc gccttcgagt gcgccgggac gccggcggcg
      121 atggtgactg cggtgaactg tgtgcggcgc ggcggccgcg tggcactggt gggtctgacc
      181 ggcaacaagc cggtcgaact caacctcgat cgtttggtgc tcgacgagat cgatgtcttc
      241 ggggtgcgct ccagcccgaa tgcgtatccc gaactgatcc agttgatcgc ggccggtaaa
      301 gtcaacgtgc gcaagctggt ctcgcgcgtt tacccgctgg agcagatcaa cgacgccttc
      361 gaagcctttc gacagcgtga gggcggcgcg atccggatgg tgatcaagat atgagccggc
      421 acgctggcga cgtgctggcc gaaatgctga tcggctatgg cgcgacggca gtcttcggac
      481 tggccggcgg gcagacgctg ccgttgtacg atgccatccg gtcgcgagcg ggtgacatcc
      541 ggcacgtgcc gctgcgggac gagcgcagcg cggcctacgc cgccgatgga tacgcgcggc
      601 tgtcaggccg ggtcggcgtg tgcgatgcca ccgtcggtcc cggcgccatc aagttcacgt
      661 ctggactggc cgaggcgctc aattcttcca ttcccatggt ggcgcttgtc agcgacatgc
      721 ccagcgactg gttggccgtt cgctatcgcg gcggcggcaa tcaactggtg gatcagttgg
      781 cggtgctcgc gccgctgtgc aagtggacgg gccgcctgcc gaaccccggc aagatgccgg
      841 aactggtcca acgtgcgttt cagatggcga ccaccggccg gccaggcccg gtggtcatcg
      901 aactgccgga ggatctcttc aaggcgagtt ttgaggcggc gaccccggcg gttgacgccc
      961 gtttcggcgc ggcgccggca ttccgccccg tgcccgatcc ggactcggtg cgggcggcgc
     1021 ttgcgctcct tgagccggcg gcccggccgg tgatgatcgc cggcggcggg gcgatgctgt
     1081 ccggcgcggg ccggtcggtg caagcgctgg ccgagcgcct ggggctgccg gtggcgacta
     1141 cgctgtcggg caagggcatc attccagaga cacatccttt ggcgctgggc gtgctgggtg
     1201 cgctcggcgg tggcgcggcg gcgcgcaaag cggtcgaaga agccgacgtg atcttcgccg
     1261 ttggcttcaa gttcgggcag aaccccacct atcgctggac cctgcccaag cggggccagc
     1321 gtatcgtcca tcttgacatc gacgccgccg agcttggcaa gatgttcccg gtcgaggtcg
     1381 gcatggtggc tgacgcccgc gcggggttgg acgcgatgtt ggcggcgagt acggccgagc
     1441 gtccgatgga cgccgtcgcg tcgaaggtgg cggcgctgcg ggccgagtgg caggcgcaac
     1501 tcgcggccga tgcgactcag gccaggccga tcaagccgca gcaggtgggg atggcgctgg
     1561 acgaagtggc cgggccggat tccgtgctgg tgtgcgatgc gagcttcgcc agcggctggg
     1621 gtggcatgta ttttacggtg cgcaacgagc gacgcgccat cttcccgcgc gggatggccg
     1681 ggttgggctg gggcttgccg gctgccatcg gcgcacagat tgcccggccg aaatcgaagg
     1741 tgatcgtcct ggccggggat ggggcgatga cgtactgtct cggcgaattg gcgactctgg
     1801 ttcaagaggg gctcaacgtc accgtcgtag tgctcaacaa cagcgctatg ggctggatca
     1861 agtgggagca ggccgtgttt tgggacggca agttccagtc gacggacttg agccaggtcg
     1921 acttcgcgat ggtggcccgc gggctgggat gcctgggcat gaacgtcagt gagccgggcg
     1981 acctgaacga ggcgctggcg caggccctgg ctgccgatgc gccggcggtg atcgacgtgc
     2041 gcacggcggc cggcgaagcg gccgtgccga agttcaccga gtctgcgcag gcgcgagcgt
     2101 tgatgcaaga taagctcgcc ggttgagggg atgagtcgtc cgcaagagac cgatgttgcc
     2161 atgcgtgaat atcgcattgc cgtgatcccc ggcgatggca ttgggaagcc ggtcatcgca
     2221 gaggggctca aggtcctgca gcgcgcggcg gccgtgcagg gcggcttcca gttgaagccc
     2281 cggcatttcg attggggctg cgaatactac ctggcacacg ggcgcatgat gcccgccgac
     2341 gcgctggagc agcttgccgg cttcgatgct atctacctgg gcgccgtggg ctatccgagc
     2401 gtgcccgatc acatttcgct gggcggcctg ctgctgccca ttcggcgcgg gttcgaccag
     2461 tacgtcaacc tacgtccgat ccagttgctg ccgggagttc aaagcccgct tcgcaccgcc
     2521 gagccgattg atttcgtgtg tgtgcgggag aataccgaag gcgaatacgc cgggatcggt
     2581 ggtcgtttct gcgccgggac ggagcacgag gtagtgacac agactgcgct cttttcacgc
     2641 cgaggcacgg aacgcgtcat ccgctatgcc tttgaattgg ctcgccagcg accgcgccgg
     2701 aagttgacga gtgccacgaa gtcgaatgcc ttgcagtaca gcatggtctt ctgggacgag
     2761 gtttttgccg aggtcgccgc cgattatcct gacgtcgaga ccgagaagtg gcatgtggat
     2821 gcgctcgcgg cgcgcttcgt cacctgcccg cagaccttcg aggtggtggt ggcctccaat
     2881 ctgtttggcg acattctcac tgacctgggc ggggcgctcc agggcagcct gggcttgccc
     2941 gccagcgcca acatcaaccc gacgcggcag tatccggcga tgttcgagcc ggtgcacggt
     3001 tcggcgcctg atatcgacgg gaaaggcatc gcgaacccca tcgctgcgat atgggccggc
     3061 gcgatgatgc tcgatgtcct gggggagacg caggccgcca ggctggtgat ggacggcgtg
     3121 cgcgcgacga ccgccgctgg gcgcacgctc acgcccgacc tgggcggtgg cgccaagacc
     3181 gtcgaagtgg gtgatgaaat ttgtcggcag atttccttat agggtgtccg atcaaagctt
     3241 gacgatggct cactgttcac tgatgactgt tcactgataa ctgtgaaaac ctggcaggtc
     3301 tagcggataa ggtgatggat caggctcatc gcacgaccgc attgtctgca aagatcggcc
     3361 tgtccgctcg cgagcagtgg ttgctggcca cgctgatgct gcccagcgcc atggagctga
     3421 tcgactccgg catgttggcc gtggcgctgc ctcgcatcca ggccgacttc ggggtgccgg
     3481 tggacgtgct gtcctgggca gtcgccgcgg gattcctgcc gcgcgtgacg ctaatgccag
     3541 tctacggcca cattggcgat ctgttcggaa agaagcgctt gttcgttctg ggcctgaccg
     3601 tctttctcgt gggagcaacg ctaggcatct ttgctccctc tttttcctgg ttggttgtgg
     3661 gtcgtttgct gcagggcctc ggcagctcca gcctgccgtt ggccatggcg ctgatcgccg
     3721 ccgagtttcc ctacgaccgc cgtgggcgag cgctaggcat ttggaatacg gccgctccgg
     3781 tgggcattat cctgggcccg gtgctgggtg gcttcatcat taatgccttt ggctggcatg
     3841 ccatgttcgt atttgtatcg ctgggatcgg cactggcgct ggctgcgacg gcctggctgc
     3901 tgccggagac aacctcggcc agtgcgcgaa gcggctttga ctgggtgggg gctgtctcgc
     3961 ttatgctggt catcctaggg ctgctgctgg cgacgacgac cgagagcctt gtcccgatag
     4021 gctccccggt gaacctggcg ttctggggca tatcggcatt ggccatagtg agcctgcttc
     4081 ggaatgcgcg ccgaaagcgg gacgccttcg tcggcctgga cattctgagg aaccggcgct
     4141 tcatgagccc tgcgctggcg gtgagtttgc gaatgttcgc gcatgacggg gcacggttcc
     4201 tgctggcgct gattctggcc aacgtattcc gccaaagtcc gaggtcggtc ggcgtgcaga
     4261 tgctcttcta cgccgtcccg ctcatgatcg gtgtagccac cggcggatat ctggccgatc
     4321 gctgggccag ccgggtggtg ggcgtggttg gcatgatcgg ccaggtggcc ggcctcttgt
     4381 ggctggctgt ggccgatgcc agtctgggcg cggctgccct ggtgccgggc atgatcgtcg
     4441 ccggcctggc cagcggcatg aacctggtcg cgctcacgaa ggaggctatc ggcgcgctcg
     4501 gggagcgacg cgtgggcctg gcctccggcc tgtataacac gatacgcttc gccgggacgg
     4561 cggcggccac gcctctgctc ggcctgctgc tggcgagcgg attcgcgcaa caagggggcg
     4621 accagactgt gagcggccct taccagctcg cgttcggtgt ggcagccggc gtggccgcgc
     4681 tcggcgctgt cgccgcagcc ctgatcccgg ccccggcccc caccccgacc ctcccccgcg
     4741 cggcagggga gggagccaag gcgccggtct cgacccgcgg aggacccagc ctatgattga
     4801 cgacagcaag atcggcgaag cgctcctcca ctggacaccg cgcttcttag ccaacggcgt
     4861 ggattacaac gaactgcagc gcatcatcgc ccgcatgcgt aagtgggacg actggtgccg
     4921 tgcctggtcc gagatcggcg ctgagcacga agcccaggcc atagtggcac tggctgccgg
     4981 tcatgcgatc acggcggccg gggcttttac tcgcgccgcc atctactacc actttgggca
     5041 gatgatcttc tacaccgaca ccgcgcagaa gaacgccgcc cacctgaaga aggtcgaatg
     5101 ctacgcgcgg gccgcgccct tgctgcgacc gccggccgtg cgcctggaga tcccgtacga
     5161 ggacatcacc ttgcctgcgt atgtgcgcct gcccgaaggc ggcgggccac acccgtgcgt
     5221 gatcctggtg tgcggcctgg actcggtcaa ggagcaggag agtcactggg aggagccgtt
     5281 gctgtcgcgg ggcatggcca cgttgagttt cgacgggcct ggtcagggtg agacctggtt
     5341 ccggatgaag atgcggctcg actacgagac ggcggtgacg gccgtgatcg acactctatc
     5401 ggcgtggccg gaaatcgacg tcgcgcgcgt cggcctgctg gggcacagca tgggcggcca
     5461 cttcggcgca cgcgcggccg cccgcgatcc acgcgtggcc gcttgcgcgc tgatcgccgg
     5521 cttcttcacg ctgcgccggt gggaggagat gtccgtcttt ctgcgggcgg gcctgaaaca
     5581 cgtttttggc gtcggcagcg aggccgaggc cagggaacga gcggcgcagc tcacgcttga
     5641 gggatttgcc ggcgacattc gttgcccact gctcatcgtg catggggcca aggatacgct
     5701 gctcccggtc gaagaagcgc accggctgaa ggccgcggtc agcggcccgg cggacctgct
     5761 cattttcccg gagggcaatc actcgtgcaa caacatgccc ttccgcgtcg agccggctgt
     5821 ggccgactgg ctcgcggatc ggctggtggc gggaccatcg cgggttggcc ggcgggaagc
     5881 gcggatttga cgaattcgcg gagggtgggt cgaggaggca tgagcgtgaa gctgaagaaa
     5941 tgcagcgttg acgacgtcga agcccggccg gcgcccaatc gccacattcg cgagctgttc
     6001 tcgggccaga cgctcggcgc gcaaggcgtg acctttcgag tggtggacat gctgccggca
     6061 tcccggcagg aaccgcgtcg accgcatagc cacccagatt ttgaggaggt gatcttcatc
     6121 ctcagcggtc aaggtcgcat ctgggccgaa ggcgaatggc tggaggtgca ggccggcgac
     6181 gcggtgctcg tcccgcccgg ggtcgtgcac gccactttca acacgaccga acagacgttg
     6241 cggctggcca gcttcttccc cgtggctgag ggtgtggacc agcgcacgag agctgctcaa
     6301 tacgtgactc tcgatctgga accaggaaat gcttagcgcc gaacgcgcat agttctggag
     6361 gatgacctca tggcaactcg cagcctgctg tccaagttgt acgtgaaagt ccttgctccc
     6421 gatcgaccgc cttaccaggt gagggacgag gaataccatc gcttcgatca gcgcaacaac
     6481 atgaccgtcg gtcgacccaa ctgggacgag agcatcaagg cctttacggg taagacggag
     6541 acgacgcgcg tgcggcgcat ccagtccggc cgtcccggct acgcggtgca ggactacagc
     6601 ctgttcctgg cgtcgggcgt cgtcgcctac cgcctgggga cgggcatcaa tcacgccaat
     6661 cgcgggttga cagcctgggc ttcgctcgga accaatctgc cgaagggggt cgatcgttgg
     6721 cagggcacgc ccgaggcggc gacggcaatg gtcaagcgcg ccgcgcattt cctgggcgcc
     6781 gatcgggtgg gcgttgcccc cctcgaccgg cgctggatct tctcgcacgc gttctggtcg
     6841 gacggctcgc acaaggagat cgtgttcgag gatgtcgaag cgcccgtgga gacggacaag
     6901 cagatgatca tccccaaccg catgcagtgg gtgatcgtga tgggcacgca gatggatccg
     6961 gagatgatga agtacacgcc gacgccggtg ggttgcgccg agacgcgcgt tacctacagc
     7021 cggctggcgc tgcaggtcgc cgggctggcg gagttcctgc gcggcgttgg ctaccaggcc
     7081 attccttcca tcaacgacct gggcctgaac attccgatgg cgatcgatgc cggcttcggc
     7141 gaacagggcc gccacggcaa gctcgtgacg ccggaattcg ggccgagcgt gcgcctgtgc
     7201 aaagtcatca cggacctgcc attggtgcgc gactacccca tccgcttcgg cgtgaccgag
     7261 ttttgcgaag tgtgccagaa atgcgcggag gcctgcccgt ccaacgcgat ctcgaccggc
     7321 gggcgagcct ggtcgggacc gaacctgtcg aacaaccctg gcgttttcac ctggcacctg
     7381 gataacgaaa gctgccggcg ctattgggag ctcggcccgg ccgacaattg cacggcttgc
     7441 atccgtgctt gccccttcac caaaggtcgc agccgtgccc acgacctgac gcggacggtc
     7501 atttcaaacg cacccaggct caaccggctg tggcgcaaac tggacgacgt gttgggctac
     7561 ggcgcggagc aggacgctac gcacttttgg gaaggcaagt gacatgacgg ataggaacaa
     7621 cggattaggc gaatggagcg gatcggatta ggcgaatgga gtggatcgga ttaggcgaat
     7681 ggagcggatc ggatcaggcg aatggagcgg atcggattag agcgaattcc tgtttggttt
     7741 acgctggagt cgaggcttta gccggaaacc gcctaaaggc tcgactccag tggagcgtaa
     7801 acggaatcgg aaaccactct aggcgaatgg agcggatcaa atgaaggcgt tggtcatcga
     7861 agaattcggc gggccgatga tccctaagga gatgccggtg cccgatcccg gcccgggcga
     7921 ggtgctgttg cgggtgaagg catgcgcggt ggatcagttc gacctgcgca tccgcgatgg
     7981 caaggtgccg catgccaggg tgccgctgat tctgggacac gaggtggctg gcgaggtagc
     8041 ggcctgcggg cagggcgtga cggagtggac ggcgggccag cgcgtcacca gtacgctgta
     8101 cctgacgtgc ggtcgctgcc gcaagtgcca gattgggcgc gagacgatct gcgagaactt
     8161 ccagggctat ctgggtatcc agactccggg cggctatgcc gagtacacgc tcgtcccggc
     8221 cgtgaatctg gtggcgctgc cggactcgat ctccttcgcc gatgcttccg tgatcgccaa
     8281 tgcgattggc acgccctatc atgctttgac gaaacgaatg cggctgcagc cgggcgagcg
     8341 ggtgatcatt accggcgcgg gcggcggtgt gggcgtgcac gcgattcaac tggcgcgcat
     8401 gatgggcgcc ctcgtcatgg ccgtggacat cggatcggcc aagctggaag cggcgcgcga
     8461 atacggcgcc gacatcgccg ccgatccgac tcaaggcgac tttgccggaa tggcgcgtga
     8521 gtggacgaaa ggcgagggcg tggaggctgt gctggaactg gtcggggcgg cgaccattcc
     8581 caccagcctg cgggcgctgg gcaagggcgg ccggctggtg attgtgggtc accacggtgg
     8641 cagcgagttc acgctgcaag ccagccaggt attggcaaac gagtgggaga tactggggtc
     8701 gcgcaacgtg tccaagcgcg agttggcgga agtcgtctcg ctggtggcgg cggggcgcgt
     8761 gcggcccatc gtcaccggtt tgcacccgct cgaagaggcc gagtcggtgc atgcacggct
     8821 tcgccagcag cagatcgtgg ggcgcga
//
LOCUS       DBNHXB010000030        21085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3865686, whole genome shotgun sequence.
ACCESSION   DBNHXB010000030 DBNHXB010000000
VERSION     DBNHXB010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 21085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21085
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3865686"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2101)
                     /locus_tag="AC162G_01180"
     CDS             complement(<1..2101)
                     /locus_tag="AC162G_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HIR5074459.1"
                     /translation="MFHLWTISNDSFDPRHLYHKETIFTIGNGYLSTRGAFEEGYPGD
                     RRATFIHGVFDEAPIVLTELANAPDWLPLTVLLNGERFSLTGGTAQAFEQTLDLRTGV
                     LTRTVRWRSPSGRVATLTFERFASLADPHTLFLRCRVTPEFEGTLEFRAALNGNTDND
                     VVTYWLAHWCWVGQGIRDGVGFLLNRTRRSKIDVACAMRLNTTIGRELARDLWDVENR
                     PTLVVKMEAVPGQEVIVDKHVAVFTSRDVDAQRVVDAAVERVSAVVGWTSALKAQVDA
                     WAEEWARTDVVIEGDDQAQLAIRFNLFQMLIAAPRHDDRVNIGAKTLSGFGYRGHSFW
                     DTEIFMLPLFTYTAPYIARNLLNYRYRNLPAARRKARANGFEGAQFPWESADTGDEVT
                     PTWLPHFADKTRLVRIWTGDIEIHISADIAYATYQYWQVTGDDEWFIDKGAELILDTA
                     RFWASRAEWNADARRYDYNDVIGPDENHEHVDNNFFTNRMAQWNLQTALGVLDWLEAH
                     APAKAAELGDKLSLDARSLAHWRDVVEKTYLPVDPSGLIEQFEGYFRRRDVDLAALEP
                     RTRSMQEVFGIEGANEIRAIKQPDVLMLLYLLGDHYSSDVVRVNYDYYTPRTDHTYGS
                     STGPPIQAIMACEVGKLDEAYDHFIRAARADLRDVRGNAGDGIHAASAAGTWQAVVFG
                     FAGLRVTPDGWTTRPRLP"
     gene            complement(2122..2745)
                     /locus_tag="AC162G_01185"
     CDS             complement(2122..2745)
                     /locus_tag="AC162G_01185"
                     /inference="COORDINATES: protein motif:HMM:NF016726.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HIR5074460.1"
                     /translation="MIKAFYVIGRAIALWWREFILLTLFNLAWLALQVPIVTGPPATA
                     AMYALARRVVDAEWIGPLDGWLALRQMFLPAWKWGAVNLVIGLVLVVNFLAYWEAPGL
                     GWVILRLLWGAIALVWFAINLFYWPFWLVQSDRRMMTTARNSLLLLVKRPAFGLTLAV
                     ISASLVVVSLLTVLPLVAVLMSWLGLIGVLAVDEELKRVNATTLSQL"
     gene            complement(2742..3680)
                     /locus_tag="AC162G_01190"
     CDS             complement(2742..3680)
                     /locus_tag="AC162G_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015189874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5074461.1"
                     /translation="MPNAALLRAKRSEERRKTIMAPVSVSRARMLRREPITLWRRVRT
                     WLSYALLFLLALSFIYPFLLASTTSFKSLPEIAADPVNLVPENWVLEGYQRMQGLSVD
                     RWAFNSAFVAVAVTLGNVLFASMAGYALARIRFPGSNLVFLGILGIMMIPGIVQLIPM
                     FIVLKQLSLIDVYGGLILPKLITPFGVFLMKQFFEAVPVELEEAARIDGANRFQMFFR
                     VVLPLARPALVALTIFSFQGNWNEFMHPLIVINTNTDLFTLPLGLAILRGGIGQSLQW
                     NAILAGSMLTTLPMALIFLFFQRYFIEGVSYAGIKG"
     gene            complement(3643..4674)
                     /locus_tag="AC162G_01195"
     CDS             complement(3643..4674)
                     /locus_tag="AC162G_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020517882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5074462.1"
                     /translation="MATGIKEQDRQDVVAGWLFMTPALIIFIVFVVIPILFAVYYSLT
                     DWNGISPPGEANFVGLKNYQAVLVENGIRQADFFKALKNTTYFALGVVPLQTALALVL
                     AVVINQRLLRFKGFFRTAYYLPAITSSIAISMVFLFFYQQSGLINRVLQTVTVGAWQP
                     IAWMNDSRGLIHIVLESLGVTLRTGPEFLRTEALGLTVWDWISGPSIALTGIMLMNTW
                     TTIGTMMIIFVAALQDVPGFVYEAAQIDGATGWTTFRKITLPLLRPTLFFVVTLGLIG
                     TYQVFDQIYVMTSGGPAKTTMTMAYMVYRNGFRNSEMGLGAAIAILLFVIIFILTLAQ
                     RRITEGKAV"
     gene            complement(4823..6130)
                     /locus_tag="AC162G_01200"
     CDS             complement(4823..6130)
                     /locus_tag="AC162G_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5074463.1"
                     /translation="MKPLLRILSLLCIGTLILAACGPAATPTAAPTTEPPEATEPPEA
                     PTEEPTEPPAAEVVELQLMGWSSSDAENTRLQAVVDTFNEANPDIKVTLNLVPDYDTK
                     LQTALAGGVPPDVFYVDSFRLPDLVDAGAIEPAEGNVENPDDFYESLRNAFTVGGTFY
                     CPPKDFSTLALEYNKDLFDAAGQEYPTADWTWEDLRAAAEALTDADAGVYGMVLSADF
                     ARWIAFLYQAGGSVTDPDFTQMTINSPEALESLNFYTNLVLDGYAAQPPDLDSGWPGE
                     AFGKARAAMAMEGNWIVPFLADQFPDVNYGLVELPAGPGGEATMAFTVCYAVPANGAH
                     KEESFRLVNFLTGEEGMKEWTDLGLAMPTRESLRSGWLEKFPDLEPFLNGAAYAHKWQ
                     FRPGFQDVLDTINNGLQQAFEGTMLPEDVLTEAEEVGNEVLNR"
     gene            complement(6311..7327)
                     /locus_tag="AC162G_01205"
     CDS             complement(6311..7327)
                     /locus_tag="AC162G_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR5074464.1"
                     /translation="MAKRVTIKDVAAQAGVSYQTVSKVINRRARVSPETEARIQTVAR
                     SLGYTPHHTARNLRAQRTWMIGYSWVPARSGQVNSVLDEFLTSMVEEAEAAGYHLLPF
                     PYREGDDQVSGYRELIDTGKVDGFVLSSVNYHDPRVRFLLERQYPFVAFGRSDPELDF
                     PYVDVDGTIGLRLVTQHLIARGHRRIAVLAWPEDSRVGDDRIAGYFAALRSARLPLDT
                     DLIARGEGRYEFGRSATQRWLAQEADRRPTAVVALNDAMAIGAMQAGQACGLAIGRDL
                     AVAGFDDSPMAQYLAPPLTSVRQPIRQAGQKCVEILTGLLEGRAPVERQVLLQPELIV
                     RASA"
     gene            complement(7409..8185)
                     /gene="cofC"
                     /locus_tag="AC162G_01210"
     CDS             complement(7409..8185)
                     /gene="cofC"
                     /locus_tag="AC162G_01210"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HIR5074465.1"
                     /translation="MGSAGAHHNWHARGRRQAARTRTHHLQGRLSGVGQIHPSLPAHA
                     QRGFTLHPTIWAVVPVKPLRRAKSRLARALKADQRAALARSVFARTLDVLASVDRIER
                     ILVMSSDLTVQDIARSKGALALAESEPGLNVALSQACAFASSRRASGVLIVPTDLPLL
                     TAHDIESLIDLACEPACLVIAPDRREEGTNALLLRPPHVIQPAFGPSSFEAHQARATI
                     AGIPVHVYRSPTLALDIDGPADLQYYGELALLLDAPPLHE"
     gene            complement(8086..8706)
                     /locus_tag="AC162G_01215"
     CDS             complement(8086..8706)
                     /locus_tag="AC162G_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR5074466.1"
                     /translation="MVDQLADTLLTLIRSRRSIRRYRPEPVPRETIERLLEAATWAPS
                     AHNRQPWRFAVVTSPEARQRLATAMGDRLRADRSRDGDPPDAIERDVKRSYERIVNAP
                     LVVVTCLSMRDMDHYPDERRASAERTMAVQSVAMAVQNLLLLAHALGLGACWMCAPLF
                     CPDTVREALRLPDDWEVQALITIGMPADEGKPRGREPIISKAVYLE"
     gene            complement(8748..9290)
                     /locus_tag="AC162G_01220"
     CDS             complement(8748..9290)
                     /locus_tag="AC162G_01220"
                     /inference="COORDINATES: protein motif:HMM:NF017020.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR5074467.1"
                     /translation="MRADTQNWIATAEYDLETARHMLATGRYLYVVFLCHLALEKMLK
                     AHVAAHSARLSRINSPRLSEADRGGASMAEATPQLTDVIQRFCEELRRMGIVCERVLL
                     FGSRAKGAASEGSDIDLFVVSSDWARYNQRERLEMLGVAAARLLEPIQARGVTPEEIA
                     THQLTPLWEQVLQEQAIVIA"
     gene            complement(9376..10113)
                     /gene="cofE"
                     /locus_tag="AC162G_01225"
     CDS             complement(9376..10113)
                     /gene="cofE"
                     /locus_tag="AC162G_01225"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HIR5074468.1"
                     /translation="MPVPGIPLIQPGDDLPGILADALKSTAPAHGDVLVVAQKVVSKA
                     ENRFVDLATVTPSARAVQLAAITQKDPRLLEVILSESDEVVRARPGLIIVRHRLGWVC
                     ANAGVDRSNVAPTGQDVVLRLPIDPDASARQIRAALCERFQMNVGVIIADSHGRPHRM
                     GTIGVAIGVAGLPGLEDWRGRRDLFGYTLEHTEVALADQIASAATLLLGQAAEAVPAV
                     LVRGVPFERRDGSARELVRPREMDLFR"
     gene            complement(10161..10838)
                     /gene="npdG"
                     /locus_tag="AC162G_01230"
     CDS             complement(10161..10838)
                     /gene="npdG"
                     /locus_tag="AC162G_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006740 - NADPH regeneration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR5074469.1"
                     /translation="MAQDTVLTLGILGGTGKQGSGLALRWAHAHYAVIVGSRDRARAE
                     ATAESINEKLGRDGVVHGMTNLEAAYACDIAVLSVPYQAQRATLESVRDALKGKILVD
                     VTVPLNPEKRRNVYIPPGGSAGAEAQALLGEEVRVVSAFQNIGFVHLQELNAPIECDV
                     LITGNDKEAKRQVTALAEAIGMRAFDAGPIENAVVAETLVSALLNINVRHKVRSAGIR
                     IVGIYET"
     gene            complement(10979..12820)
                     /locus_tag="AC162G_01235"
     CDS             complement(10979..12820)
                     /locus_tag="AC162G_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074470.1"
                     /translation="MRARHRLILPLAGLLISMLAFTDTAWLSAQARPPNRSESAVLAG
                     CGIVSCTTYLPVLARDFPSPWPIEVTQGVQQPDNSVTLIQDRPTFVRLTITSTTPHTG
                     VSASLHGMRGGAPLPGSPIPAINNPRALESSADRAVLNDTFNFELPASWLNGTITLYG
                     AAANGTTFNVTGGSSSFTFVNADPLRVTVLPISYVCTSGGSGTTTPQGPYTYLTEYTY
                     RTYPVPSLAMATHAALIFNGGPCTPSGVPDPDFYEWDTMLDLVTTVWVSEGRRDSYYY
                     GLVKVYCGSSCIAGIGWIGYSKAAVGFDGVNAGHSGAGPTHAHEVGHNHGRSHAPGCG
                     AAGIDSAFPYIFGGRGYIGNSANPNFGFDAGTQTIYPYSPYYDFMGYCDPTWISDYTF
                     EALRGWNQAHRAGDRPGVERQRVMLVSGRLSQDGSMAISPVYTLDASALLPEPGDYIL
                     ELLDAAGRIVAAYPFAPGQAVADRWRAGTAFERAGFHMALPYVAGVKAMRVRRGAAVL
                     GLRQADLRKPSLVAGRNALSTDGQSVVFDWVGSDGDGEMPVYLVRASTDGGTSWQTIG
                     VNLTTPSIELSLADFAGESVLVEVLGSDGLNTTSLMLGPYNAPAYDN"
     gene            complement(12920..13288)
                     /locus_tag="AC162G_01240"
     CDS             complement(12920..13288)
                     /locus_tag="AC162G_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074471.1"
                     /translation="MTAQNDQPSSIQDTQKAALNKRLESIGWGLFLIMIGGIWLVPDE
                     QVPGGTWLVGAGLIMLGLNAARYLNGIRMSGFTIVLGILALASGLSDFAGIDLPLFPI
                     LLILIGASILLKPVFERRQG"
     gene            13634..15559
                     /locus_tag="AC162G_01245"
     CDS             13634..15559
                     /locus_tag="AC162G_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5074472.1"
                     /translation="MSIVLEQLTKRYDGHPVVNHVSLEIADGELFVLLGPSGSGKSTI
                     LRMIAGLTDSDEGRMVLHGRDVTYLSPQQRGVGFVFQHYALFRHMSVAANVEFALRIR
                     KVSAAERRKRRDELLELVGLAGLGGRMPSQLSGGQQQRVALARALAHRPDVLLLDEPF
                     GALDAKIRVELRRALRAIQEELGMTTIFVTHDQEEAFELADRLGVMNFGRLLEVGQPE
                     ELYQRPQTEFVATFLGTANLLVGQATPTGVQLGPVHFPLSTVATPAAEQRVQVLFRTE
                     DVALAESSDLLGCPHIGCGEVEHTTFAGSFEKLRLRLPPLPGVRPISPPVSFGDNSIL
                     VEATRSQDLTHRFPLTPGSKVWVGVRRIHALAHPGLSFLFLTDGSPQAKAALDVGGQI
                     ARMAHARVTILGFGLAEETLQRHLQEVKEQLGSGLASLDLRATDDDAVEAVGREIERQ
                     PFDLIVLGRQLRGGVELAEELMQSGEHHLLLVPGPQPAPSRALVCVTSGEPGKHDVLF
                     AGRLIRHLGAEATLLSVMRPGDDTLRRNQTERFLAGGVRSLEVLGVQAKSKIRFGAVR
                     DEVVEEVNEGQYDLLVLGAPLPDREGRISLNGLVGQVLSGVADRATLIVRSSYTTAKL
                     PILVPLGRLSLVEEDQL"
     gene            15556..16599
                     /locus_tag="AC162G_01250"
     CDS             15556..16599
                     /locus_tag="AC162G_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5074473.1"
                     /translation="MKKRIVSTLVVLALIGLLAACTGPAATQPSSQGSIKLILGAYTT
                     PREAYGKLIPLFQAQWKKQAGQDVTFEESYLGSGAQSRAIVEGFEADVAALSLEGDIT
                     RIEKAGLITQDWKSGTYKGMVSTSIVVFGVRKGNPKGIQDWADLAQPGLEVLTPDPKS
                     SGGAQWNIVALYGAAIRGKISGVPAGDEAAALEFLKTVLRNVTVFDKGARESITNFEK
                     GVGDVIITYENEILVGQKNGQDYEMIFPQSTILIENPVAVIDTYVDKHGTRAAAEAFV
                     DFLFTRQAQEIFAEYGLRSVDPEVAQATASQYPTIEDLFTIRDFGGWSEVADKFFDDD
                     GIYSVAIAQVQGQ"
     gene            16672..17532
                     /gene="cysT"
                     /locus_tag="AC162G_01255"
     CDS             16672..17532
                     /gene="cysT"
                     /locus_tag="AC162G_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HIR5074474.1"
                     /translation="MATTSLTATQSPPRISAPWGRWGVRGIAISYLALMLIIPLVVIF
                     QDGLREGLAGVWEAASKPIAASALRLTLWTAAIMALINGAMGLLTAYVLVRYDFRGKS
                     ILNAIIDLPLAIPTLVTGVMLVVLYGPQSAVGSWLDSTLGLRVIFAPPGIILALLFIT
                     FPFVVRAIQPVLLDLERAQEEAAATLGAGGLTIFWRIVLPRLAVPMISGMLLSFARAI
                     GEFGSIVIVAGNIPFKSQTAAVYVLGEVESENRLAASALSIVMLSIAFGLVLLVSRLQ
                     KRGHKRAESS"
     gene            17611..18441
                     /locus_tag="AC162G_01260"
     CDS             17611..18441
                     /locus_tag="AC162G_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HIR5074475.1"
                     /translation="MTSLAVAVPQRRAGRWGQRLLIGVVLAYVAFLILAPIAALVAGA
                     LQDGLGSALAALAAPDLLAAFGQTLSIGLIVVCVHVVFGTAVAWVLVRHRLPGRNLLN
                     GLIDLPFAVSPVVVGYMLLLLFGRRGVLAPILQTLDIQVAFAVPGMILATLFVSLPFM
                     IRELMPILEEFDIEQERAAATLGASGWQTFRLVTLPALRWGFVYGIVLTFARALGEFG
                     AVLVIGGGVQGRTETATLYIFRALDERQYVGAFSAALVLGLCSLLLVLGADLIRRKQG
                     "
     gene            18512..20575
                     /locus_tag="AC162G_01265"
     CDS             18512..20575
                     /locus_tag="AC162G_01265"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5074476.1"
                     /translation="MALRNPLRGLRLSALLIGINVGLLLLAVAGVAAVAVSLLQRLAD
                     EQALARVAQAGASAQNAIERAGDEILRDAQLLAERPTLLRLIQSSDAQALSAFLSQFQ
                     LTSQLDATAIIVSDRVFASSGAALPWDALWPEHQDESGYYFHRQAEGALLIMGAQVEV
                     PSLTGATVLAAKLLDESFAAQIASEVGLTVTILERESALADVDLRQLRSAATGQTVTA
                     RRDDLGVYLAVSPLRAPSGQIAGFVETVLPSTLLADSLRRLIETLVALTLGVAALAAL
                     VSFLFGRWLVHPLLRLRNAAARIGHGDLATPVVVVSGGEIGTLAVTLEDMRRRLLQLT
                     ADLKRQQAESNAIVTSIIEGVFTVDRERRIRYLNPQAAVLLGVNAEEAIGQFCGNVLN
                     PQGPDQVRPCEEQCPIVHARFRAGASATEHLLLRHGARRTVVITSAPPSENRQVQVIR
                     DETEVEATRRLRDAVLANISHEFRTPLSAQLASIELLLDQLPDLSTEQIGTLILSLQR
                     GTLRLTQLIDNLLESVRIEAGEHAIRRQPVLLDEVIEQALEFTQPLLAQREQEVEIEL
                     PYPIPAISGDATRLTQVFVNLLANANKFAPAGSTIRIGGAVEPTTIRVWVEDQGPGIP
                     PQAGQSLFTRFVRSTGEEPEQGGVGLGLWIVKSIVERHGGHVEAFSDSTGTRMRMTLP
                     RGNES"
     gene            20572..>21085
                     /locus_tag="AC162G_01270"
     CDS             20572..>21085
                     /locus_tag="AC162G_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5074477.1"
                     /translation="MKILAVDDDRELLNLISFALRQSGYLVIEASNGDAALSAFEREQ
                     PDLVILDVNLPGLSGFDVCRRMREQASTPIMMLTVRSSEEDEVKGLDLGADDYLTKPF
                     SPRTLLARVRALLRRADAERPAPALGAGDVVLDVEMQTVSVRGGAPVRLTNLEFRLLQ
                     YLLANAGRALS"
BASE COUNT         3924 a         6472 c         6834 g         3855 t
ORIGIN      
        1 tgggcaggcg cgggcgcgtc gtccagccgt cgggcgtcac gcgcaggccg gcgaagccga
       61 agacgactgc ctgccacgtc ccggctgccg aagccgcgtg gatgccgtcg ccggcgttgc
      121 cgcgcacgtc gcgcaggtcg gcgcgggccg cgcggatgaa gtggtcatac gcctcatcga
      181 gtttgcccac ctcacaggcc atgatggctt gaatgggtgg gccggtcgac gagccgtagg
      241 tgtgatcggt gcgcggtgtg tagtagtcgt aattgacgcg caccacgtcg gagctgtagt
      301 ggtcgcccag gagatagagg agcatgagca cgtccggctg tttgatcgcc cggatctcgt
      361 tggcgccctc gatgccgaac acctcctgca tcgacctggt gcgcggctcg agcgcggcaa
      421 ggtcgacgtc cctgcgccga aaatagccct caaactgctc gatcagaccg gatggatcga
      481 ctggcagata cgttttttcg acaacatccc gccagtgagc gagagatcgc gcgtcgagcg
      541 agagtttgtc gccgagttca gccgctttgg ccggcgcgtg tgcttcgagc cagtccagca
      601 cgccaagcgc agtctgcagg ttccactgcg ccatgcggtt cgtgaagaag ttgttgtcca
      661 cgtgctcgtg gttctcgtcg ggtccgatca cgtcgttgta atcgtagcgc ctggcgtccg
      721 cgttccactc ggcgcgcgag gcccaaaatc tagcagtatc caggatcaat tctgcgcctt
      781 tgtcgatgaa ccactcgtcg tcgccggtca cctgccagta ttgatatgtc gcgtaagcga
      841 tgtccgccga gatgtgaatc tcaatgtcgc cggtccagat gcgcacgagc ctggtcttgt
      901 cggcaaaatg cggcagccag gttggcgtca cttcgtcgcc ggtatccgcg ctttcccatg
      961 ggaactgtgc gccctcgaaa ccgttggccc gcgccttgcg ccgcgcggcg ggcaagttgc
     1021 gatagcggta attgagcagg ttcctggcga tgtacggcgc ggtgtacgtg aacagcggaa
     1081 gcatgaagat ttccgtgtcc cagaacgagt gcccgcgata gccgaagcct gaaagcgtct
     1141 tggccccgat gttcacgcgg tcgtcgtgcc gtggcgcggc gatgagcatc tggaagagat
     1201 tgaagcggat cgcgagctgc gcttggtcgt cgccctcgat gaccacatca gtgcgcgccc
     1261 attcttcggc ccacgcgtcg acctgggctt tcagtgccga cgtccaaccg acgacggcgc
     1321 tgacgcgctc gacggcggcg tctaccacac gctgcgcatc cacgtcgcgc gaggtgaaga
     1381 cggcgacgtg tttatcgact atcacttcct ggccggggac ggcctccatt ttcacgacga
     1441 gggttggcct attctcgacg tcccacaggt cgcgggccag ttcgcggccg atggtcgtgt
     1501 tcagccgcat ggcgcaggca acgtcgattt tggatcggcg cgtgcggttg agcaggaagc
     1561 ccacgccatc gcggatgccc tgacctaccc agcaccagtg ggccagccag tacgtcacca
     1621 catcattgtc cgtgttaccg ttgagcgcgg cgcgaaattc cagcgtgcct tcgaactcgg
     1681 gcgttacgcg gcagcgcagg aacaacgtat gcggatcggc gagagacgcg aaacgctcaa
     1741 aggtcagggt tgccacccgg ccggaggggg aacgccagcg cacggtgcga gtcagcacgc
     1801 cggtgcggag gtcaagcgtt tgctcaaagg cctgcgccgt gccaccggtc agcgagaagc
     1861 gttcgccgtt cagcagcacg gtgagaggca gccaatccgg cgcgttggcc agttcggtga
     1921 ggactatcgg agcctcgtcg aacacaccgt gaatgaacgt cgcccgccgg tcgccgggat
     1981 agccttcttc gaacgcgccc cgtgtcgaga ggtagccgtt gccaatggtg aagatggttt
     2041 ctttgtggta gagatgacgt gggtcgaacg aatcattgga gatggtccag agatgaaaca
     2101 tgacaggagt tcacccatcg gtcaaagttg agagagtgtt gtggcattga cccgcttcag
     2161 ttcttcatcg actgccaaca cgccaatcag ccccaaccag gacatcagga ccgcgaccag
     2221 cgggagtaca gtcagaagac tgaccacgac caagctggcg ctgatgactg caagcgtcaa
     2281 accgaaggcc ggccgcttga ccagcaagag caggctgttg cgggccgtgg tcatcatgcg
     2341 ccggtcagac tgcactaacc agaatggcca atagaacaga ttgatcgcaa accagacaag
     2401 tgcaatcgcg ccccacagca gccgcaagat cacccaaccc aggcctggcg cttcccagta
     2461 agccaagaag ttgacgacga gcacaagacc gatcaccaag ttgacggcac cccatttcca
     2521 ggcgggtaga aacatttggc gcaacgccaa ccagccgtcg agggggccta tccattcggc
     2581 gtcgactacg cgccgggcaa gggcgtacat ggccgcggtg gcgggcgggc cggtgacgat
     2641 gggaacttgc agcgccaacc aggccagatt gaacaaggtg agcagtatga actcacgcca
     2701 ccacaaggcg atggcacggc cgatgacata gaatgccttg atcatccctt tatgccggcg
     2761 taactcacgc cttcgatgaa gtagcgctgg aaaaagagga agatcaaggc catcggtaga
     2821 gtggtgagca tcgatccggc cagaatggca ttccactgca aactttgacc tatgccgcct
     2881 cgcagtatgg ccaggcccag aggcagggtg aagagatccg tgttggtatt gatcacgatc
     2941 aggggatgca tgaattcatt ccagtttccc tggaaactga agatcgtgag cgccaccagg
     3001 gccgggcgag ccaagggcag caccacgcgg aagaacatct gaaagcgatt ggcgccatcg
     3061 atacgggccg cttcctcaag ctcgacgggc acggcctcaa aaaactgttt catcaagaag
     3121 acgccgaacg gcgtaatcag cttgggcaaa atcaggccgc cgtagacatc gatcaacgat
     3181 agctgtttga gcacaatgaa catggggatc aactgcacga ttccaggaat catcatgatg
     3241 cccaggatgc ccaggaagac aaggttgctg ccgggaaagc ggatgcgggc cagggcatag
     3301 ccagccatgc tcgcaaacag cacgttgccc agggtgacgg cgacagcaac gaacgcgctg
     3361 ttgaaggccc agcggtctac gctgaggccc tgcatccgct gatagccttc caggacccag
     3421 ttttcgggca ctaagttgac tggatccgca gcgatctcag gcagagactt gaagctggtg
     3481 gtcgaggcca gcagaaatgg gtagatgaag gagagagcca ggagaaagag cagcgcgtag
     3541 gagagccagg tgcggacacg cctccacaac gtgatcggct cgcgccggag catgcgagcc
     3601 ctcgatacgg atacaggcgc catgatggtc ttcctccttt cttcagaccg ctttgccctc
     3661 agtaatgcgg cgttgggcaa gagtcaggat gaagatgatg acaaagagca gaatagcgat
     3721 ggctgcgcct aggcccatct cgctgtttcg gaagccgttc cgatagacca tgtaagccat
     3781 cgtcatggtg gtttttgccg ggccaccact cgtcatcacg tagatctgat cgaacacctg
     3841 gtaagtaccg atcaagccaa gcgtgaccac gaagaagagg gtgggacgca gtaacggcag
     3901 cgtgatcttc cgaaatgtgg tccagcctgt ggcgccatcg atctgagcgg cctcatagac
     3961 aaagcccgga acgtcctgca aagccgcgac gaaaatgatc atcatggtgc cgatcgtggt
     4021 ccaggtgttc atcagcatga tgccggtgag cgcgatgctg gggccgctga tccaatccca
     4081 gaccgtcaaa cccagggcct cggttcgcag gaattcgggg ccggtgcgca gggtgacccc
     4141 cagactctcc aggacgatgt ggatcagccc tcgggagtcg ttcatccaag cgataggctg
     4201 ccaggcgccg acggtcaccg tttgcagcac ccgattgatc aggccactct gctgatagaa
     4261 gaagagaaac accatgctga tggcgatgga cgaggtgatg gcgggcagat agtaggccgt
     4321 gcgaaagaaa cccttgaaac gcagcaagcg ctgattgatc accaccgcca gaaccagagc
     4381 cagggctgtc tgcaagggca cgacccccag agcaaaatag gtcgtgttct tcagggcttt
     4441 gaagaagtcg gcctggcgga taccattctc aaccagcacc gcctggtaat tcttcagacc
     4501 gacgaagttg gcctcgccgg gcgggctgat gccgttccag tcggtcagac tgtaatagac
     4561 ggcgaacagg atggggatga cgacgaagac tatgaagata atcaaggccg gcgtcataaa
     4621 cagccagccg gcgacgacat cctggcgatc ctgttcttta attccagtcg ccatagtgca
     4681 cctcgcagaa acggggtgaa cggcagacag tgactagcca gcagtcagtg gcgaatggcg
     4741 aaccgacaac tgaacatcag ggaacggcga ccgggacgcg cgccacatgt tcccggtcgc
     4801 cgtttaccga tcgcgatgcg cctcagcggt tcagcacctc attgcctact tcctcggcct
     4861 cagtcagcac gtcctcggga agcatcgtgc cttcaaaggc ctgttgcagg ccgttgttga
     4921 tcgtgtctag cacatcctgg aagccagggc ggaactgcca cttatgggcg taggcagcgc
     4981 cgttcaggaa aggctccaga tcagggaatt tctccagcca gccagatcgc aggctttcgc
     5041 gagtaggcat ggccaggccc agatcggtcc attccttcat gccttcctca ccggtcagga
     5101 aattgaccag cctgaaggac tcttctttgt gagcgccatt ggccggcaca gcataacaca
     5161 cggtgaaggc cattgtcgcc tcgcctccag ggccggccgg cagctccacc aacccgtagt
     5221 tcacatcggg gaactgatcg gccaggaagg gcacgatcca gttgccttcc atggccatcg
     5281 cggccctggc cttgccgaag gcttcgccag gccatccgct gtccaggtca ggcggctgag
     5341 ccgcataacc atccaacacc aggttggtgt agaagttcaa ggactccagc gcctctggac
     5401 tgttgatggt catttgggtg aaatcggggt cggtcacgct gccgccagcc tggtacagga
     5461 aagcgatcca gcgggcgaag tcggcgctga gcaccatgcc gtagacaccg gcgtcagcgt
     5521 cggtcagggc ttcggctgcc gcacgcaagt cctcccaggt ccaatcggcg gtgggatact
     5581 cctgtccggc tgcgtcgaag aggtctttgt tgtactcgag ggccaacgtg ctgaagtcct
     5641 tgggtgggca gtagaacgtt ccgcccacgg tgaaggcgtt acgcaggctc tcgtagaaat
     5701 catcggggtt ctcgacgttg ccttcagccg gttctatcgc gccggcgtcc accagatccg
     5761 gcagtctgaa gctgtcgaca tagaagacgt caggcggaac accccccgcc agagcggtct
     5821 gcagcttggt gtcgtagtcg gggaccagat tcaaggtgac cttgatatcg ggattggcct
     5881 cgttgaaggt gtccacgaca gcctgaagcc gggtgttctc ggcgtcggaa ctagaccacc
     5941 ccatcaactg aagctcgacc acctcggcgg ccgggggttc agtcggctcc tccgttgggg
     6001 cttcgggcgg ttcagtagcc tcgggcggtt cggtggtcgg agcagcggtc ggggtagctg
     6061 ccggcccaca ggccgccagg atcaacgtcc caatgcacag cagggacagg attctaagca
     6121 gcggtttcat gggaattctc ctccatcgtg atgcagcatg atttgcccac gttgggcaaa
     6181 agcgaaatgc gtggtaaagg ctcacgctcc agttcttgca tcgtcagatg gcctcacctc
     6241 cttatgtgtg taagccgggc gtccctcatc gaatacgaac ctgatgcttc tgtactgcca
     6301 tgcttcaatt ctaggccgaa gcccgcacga ttaactccgg ctgcaacagc acctgccgct
     6361 caaccggcgc gcgtccttcc aacagcccgg tcaatatctc cacacacttc tgtccggcct
     6421 gccggatggg ttggcgcacg ctcgtcaacg gtggcgcaag atattgggcc attggggaat
     6481 catcgaagcc ggcgacagcc agatcacgac caatcgcaag gccgcatgcc tgcccggcct
     6541 gcatggcgcc aatcgccatg gcgtcgttca gggcgacaac agcggtcgga cggcgatccg
     6601 cttcctgcgc cagccagcgc tgggtcgcgg aacggccaaa ctcgtaacga ccttctccac
     6661 gcgcgatcaa gtccgtatca agtgggagcc gcgccgagcg caaagcggcg aagtagcctg
     6721 ctatccgatc gtcaccgacg cgagaatctt cgggccaggc cagcacggcg atgcgccgat
     6781 ggcctcgcgc aatcaggtgc tgtgtgacga gccgcagccc gattgtgccg tctacatcta
     6841 cataaggaaa gtccagttct ggatccgagc ggccaaaagc gacgaaggga tattgcctct
     6901 ccagcaaaaa gcgcacacgt ggatcgtggt agttcacact ggacaggaca aaaccatcta
     6961 ctttgcccgt atcgatcagt tcgcgatagc cggatacctg gtcgtcgccc tcgcggtacg
     7021 ggaagggcag caaatgatac ccggcggcct cggcctcctc aaccatactg gtgagaaatt
     7081 cgtcgaggac ggagttaacc tgtccggacc gagccggcac ccacgaatac ccgatcatcc
     7141 aggtgcgctg ggcgcgcaaa ttgcgggcgg tatggtgagg tgtgtagccc agtgagcgcg
     7201 cgacggtctg aattcgagcc tcggtctccg gggatacacg cgcacgtcga ttaatgacct
     7261 tggagacggt ctggtagctg acgcccgctt gcgccgcaac gtccttgatg gtgactcgtt
     7321 tagccatact tgggcgaacg ttcgcctaag tagtatataa cgagatttgg gtagtagtca
     7381 agtacggatt ttcggaacaa cgtgtatgct actcgtgaag cggcggcgcg tcaagcagca
     7441 gcgcgagctc tccatagtac tgcaggtcgg ctggcccgtc gatgtcgagc gcgagcgtcg
     7501 gcgatcgata aacgtgcacc gggatgccgg ctatggtggc tcgcgcctgg tgcgcctcga
     7561 atgacgacgg gccgaacgcc ggctggatga cgtgcggcgg gcgcagcagc agcgcgttgg
     7621 tgccttcctc gcgccgatcc ggggcgatga cgaggcacgc tggctcgcaa gccagatcga
     7681 tcaacgactc gatgtcatgc gccgtgagca gtggcaggtc cgtcgggacg atgagcacgc
     7741 cggacgcgcg gcgcgacgat gcgaacgcgc acgcctgcga cagcgcgacg ttgaggcccg
     7801 gctcgctttc ggcgagggcc agcgcgccct tggagcgcgc gatgtcttgc acggtcagat
     7861 cgctgctcat gacgaggatg cgctcgatcc ggtcgactga ggccagcacg tcgagcgtgc
     7921 gcgcgaacac ggagcgcgcc agcgccgcgc gctggtccgc tttcagcgcg cgcgccaggc
     7981 ggctcttggc gcggcgcagc ggcttgacgg ggacgacagc ccatatcgtg ggatgaaggg
     8041 tgaacccccg ctgcgcgtgg gcaggtaatg aaggatgaat ctgacctact ccagataaac
     8101 ggccttggag atgatgggtt cgcgtccgcg cggcttgcct tcgtccgcgg gcatgccaat
     8161 tgtgatgagc gcctgcactt cccaatcgtc cgggagtcgt agtgcttcac gcacggtgtc
     8221 cgggcagaac agcggtgcac acatccagca cgcgccgagt cccagcgcgt gcgccaggag
     8281 cagcaggttt tgcacggcca tcgcgacgct ctgcacggcc atcgtccgct cggcggaggc
     8341 gcgccgctcg tcggggtagt ggtccatgtc tcgcatcgac aggcaggtga cgacgacaag
     8401 cggggcgttc acgatacgct cgtaagagcg cttcacgtcc cgctcgatgg catccggtgg
     8461 atcgccgtcg cgagatcgat cggcacgaag gcgatcgccc attgccgtgg ccagccgctg
     8521 tctggcctca ggggacgtga cgacggcgaa gcgccacggc tggcggttgt gcgcggatgg
     8581 cgcccacgtc gccgcttcca gaagccgctc gatcgtctcg cgcggaacgg gttcgggacg
     8641 atagcggcgg atggagcggc gcgagcggat cagggtgagc agagtgtccg cgagttgatc
     8701 gaccatgtgt agccgcttgc ccctcggttc aggccttctt cgtggtgtta cgctattacg
     8761 atagcctgtt cctgcagcac ctgttcccag agcggtgtca attggtgcgt ggcaatttcc
     8821 tcaggcgtga ctccgcgtgc ctggataggc tccaggaggc gcgctgcagc aacgcctagc
     8881 atttccagcc gttcgcgctg gttgtagcgt gcccagtccg acgagacaac gaacaaatca
     8941 atgtcactgc cctcactggc tgcacccttg gctcgagagc caaatagcag cacacgctcg
     9001 cagacaatcc ccatccttct cagctcttca cagaagcgct gaatcacgtc tgtaagttgg
     9061 ggtgttgctt cagccatcga agcacctcct ctgtctgcct cagatagtcg cggactattg
     9121 attcgggata gtcgcgcaga gtgcgctgca acgtgggctt tcagcatctt ctccagagcc
     9181 agatggcaga gaaacacaac atagagatag cgacccgtcg ccagcatgtg ccgcgcggtt
     9241 tccagatcgt attcggctgt agcgatccag ttctgtgtat cggctctcac acagtacctc
     9301 ctgaacccaa tgcctcctca acctgcttca ccaccgtccg ctgtggtgag ccatcgccgg
     9361 gctctcgttc gtcgatcatc taaacagatc catctctctc ggcctcacca gttcccgcgc
     9421 cgagccgtcg cgccgctcga aaggaacgcc tcgcacgagg acagccggta ccgcttcggc
     9481 ggcttgtccg agcagcagcg tcgccgccga ggcgatctga tccgctagcg cgacctcagt
     9541 gtgctcgagg gtatagccga acaggtccct tcgcccgcgc cagtcttcca acccgggcaa
     9601 accggcgact ccgatcgcga ctccgatcgt gcccatgcgg tgcggccggc cgtgactgtc
     9661 ggcgataatt accccgacgt tcatctggaa gcgctcgcac agcgcagcgc gtatctgccg
     9721 ggcagacgcg tctggatcga tcgggagtcg cagcacgacg tcttgccctg tcggcgccac
     9781 gttgctgcga tcgacgccgg cgtttgcgca tacccagccg agccggtggc ggacgatgat
     9841 caagccgggg cgcgcgcgaa caacttcgtc gctctccgat agaatcactt cgagcaggcg
     9901 tgggtctttt tgtgtgattg ccgcaagctg cacggcgcgc gcagatggtg tgaccgtcgc
     9961 cagatcgacg aagcggttct ccgccttgct gacgaccttc tgcgcgacga ccagcacgtc
    10021 accgtgggcc ggcgccgttg acttcaatgc gtcggctaga attcccggca ggtcgtctcc
    10081 gggctggatg agaggaatgc cgggtaccgg cacgagcgta atcagtttgc caatccacgc
    10141 cgagcggtgc gaaatatgag ctaggtctcg tagatgccga cgatgcggat gccggcggac
    10201 ctgaccttgt gcctcacgtt gatgttgaga agggccgaca ccagtgtttc ggccaccact
    10261 gcattctcga tcgggccagc atcaaacgct cgcatgccga tcgcctcggc cagtgcggtg
    10321 acctgccgct tcgcctcttt gtcgttgccg gtgatcagca catcgcactc gatcggcgca
    10381 ttgagttcct gcaagtggac aaaaccgatg ttttggaacg ccgacaccac tctgacctct
    10441 tcgcccagca gcgcctgggc ctctgccccg gccgagccgc ccggtggaat atacacattg
    10501 cggcgcttct cagggttgag tggcacggtc acgtcgacga gaatcttgcc cttgagcgcg
    10561 tctctgaccg attccagtgt cgcgcgctgc gcctggtatg gcacgctcaa tacggcaata
    10621 tcgcaggcat aggctgcctc gaggttcgtc atgccgtgca cgacgccgtc tcggccgagc
    10681 ttttcgttga tcgattcggc cgtggcctcg gcgcgcgccc ggtcacgcga gccgacgatc
    10741 accgcatagt gagcgtgcgc ccagcgcagc gccagccccg atccttgctt gcccgtgccg
    10801 cccaggatgc ctagcgtgag aacagtgtcc tgtgccattg actcgtctct cggtgaggtt
    10861 agggtgatta tatcagagat aagcgcctgt ccgaaaacga gcgggctggg ctcccacgcc
    10921 cggcaaaccg gcggcatggg agccgcctcc gctaaggtct aagactgccc ggtgtgtatc
    10981 agttgtcgta cgcgggcgca ttgtacgggc cgagcatcag cgacgtcgtg ttcagtccat
    11041 cgctgcccag aacctcgacc aggacgctct cgccggcaaa atcggcaagg ctcagttcaa
    11101 tcgaaggcgt ggtcaggttc acgccgatcg tctgccacga cgtgccgccg tctgtcgagg
    11161 cccgcaccag gtagaccggc atctcgccgt cgccgtccga gccgacccaa tcgaagacga
    11221 ccgattgccc gtctgtgctg agcgcatttc ttccggcgac gagcgatggc ttgcgcaggt
    11281 ctgcctgccg caagccaaga actgctgcgc cgcgccgaac gcgcatagcc ttgacgccag
    11341 cgacgtaagg cagggccatg tgaaaaccgg ctcgctcgaa ggcggttccg gctcgccacc
    11401 ggtcggccac ggcctgcccg ggggcgaagg ggtaggcggc gacgatccgg cccgccgcgt
    11461 cgagcaactc gaggatgtag tcacccggct ccggcagcag ggcactcgcg tcgagcgtgt
    11521 agaccgggga gatcgccatc gagccgtcct ggctgagccg gccggacacg agcatcacac
    11581 gctgccgctc cacgcccggc cgatcgcccg cgcgatgcgc ttgattccag ccccgcagcg
    11641 cttcgaaggt atagtccgag atccacgtcg ggtcgcagta gcccatgaaa tcgtagtacg
    11701 gcgaataggg atagatcgtt tgcgtgccgg cgtcgaagcc gaagttgggg ttggcgctgt
    11761 tgccgatgta gcctctgccg ccaaatatgt agggaaacgc cgagtcgatt ccggccgcgc
    11821 cgcagccggg cgcgtgtgat cgcccgtggt tgtgcccgac ctcgtgagcg tgcgtcgggc
    11881 ctgcgccgct gtgcccggcg ttgacgccgt cgaagccgac ggcggctttg ctgtacccaa
    11941 tccagccgat accggcaata caactgctgc cgcagtatac cttgacaagc ccgtagtagt
    12001 agctgtcccg gcggccttcg ctcacccaca cggtcgtcac caggtcgagc atggtgtccc
    12061 attcgtagaa gtcgggatcg ggcacgccag atggggtgca aggcccgccg ttgaaaatca
    12121 gcgccgcgtg cgtggccatc gcgagcgaag gcacgggata cgtgcgatac gtgtactcgg
    12181 tgaggtaggt gtaagggcct tgcggggtcg tcgttccgct cccgccactc gtgcacacat
    12241 aggaaatcgg cagaaccgtg acgcgcagcg gatcggcgtt cacgaacgtg aacgaagatg
    12301 atccgccggt tacgttgaag gtcgttccat tggccgcagc gccgtacagc gtgatcgtcc
    12361 cgttcagcca cgacgccggc aactcgaagt tgaacgtgtc gttcaaaaca gcccgatcgg
    12421 cgcttgattc gagcgcgcgc gggttgttga tggcagggat gggcgatccg ggcagaggcg
    12481 caccgccgcg cattccgtgc agggaggcgc tgacgcccgt gtgcggagtc gtcgacgtga
    12541 tggtcaggcg gacgaacgtg ggtcgatcct gtatcagtgt gacggagttg tccggctgct
    12601 gcacgccctg cgtcacctcg atcggccacg gggacgggaa atcgcgagcc aataccggca
    12661 ggtaagtcgt gcaactgacg atgccgcagc cggccagaac cgccgattcg ctccgattgg
    12721 ggggccgtgc ctgggcgctc aaccacgccg tgtctgtgaa ggcgagcatg ctgatcaaca
    12781 gcccggccag cgggaggatt agtcggtgac gtgcgcgcaa gaatgtgcct ttatccaatg
    12841 gcagccacac gttgggatta caagcgttgg tgaattgtag cacgcacgag cgggccgcga
    12901 caatgcgacg gactacctat cagccctgcc ttctctcgaa caccggcttg agaagaatgc
    12961 tcgctccgat caggatgagc aggatcggga agagcggcaa gtcgatgccg gcgaagtcgc
    13021 tcagcccaga ggccagcgcc aggatgccca acacgatcgt gaagccgctc atccggatgc
    13081 cgttgaggta gcgggcggcg ttgagtccaa gcatgatcag gccggccccg accagccacg
    13141 tgccccccgg cacctgttcg tctggcacga gccagatgcc gccgatcatg atgaggaaca
    13201 gcccccagcc gatgctttcg agacgcttgt tcagtgcggc tttctgtgtg tcttggatgc
    13261 tcgagggttg gtcgttctgt gcggtcatgg tatccccccc tgtgtcgtga gatatccatt
    13321 ccagtataag gcattcccta tgaaattgaa actcacgcct gaccgcaaat ataaccgccg
    13381 ggtagtacag taatcatagc ttgacaatgc cagatccggc caacgccgcc ggagcgtgcc
    13441 ctgcgaagtc cgccctgagc gtcgtcgaag ggccgaaggg acggcggctt gagcctaact
    13501 gtgacattgt cgaaataggt ctttcgggtt tacgggaaac cggcagtgtg tcatacggcc
    13561 gtaagcatgg aaaagctttg tcaccagatt gtcaccgctt tggatccgcc ctgagaccgc
    13621 aagaaggcaa actatgtcca tcgtcctcga gcaactcacc aagcgctacg acggccatcc
    13681 ggtcgtcaac cacgtctcgc tggagatcgc ggacggcgag ctgttcgtgc tgctcgggcc
    13741 aagtggcagc ggcaagagca ccatcctgcg catgatcgcc ggcctgaccg acagcgacga
    13801 agggcgcatg gtgctccacg ggcgcgacgt gacatatctg tcaccgcagc agcgcggtgt
    13861 gggtttcgtg tttcaacact acgcgctgtt ccgccacatg agcgtggccg cgaacgtcga
    13921 gtttgcgctg cgcatccgca aagtatctgc cgccgagcgg cgcaagcgac gcgacgaact
    13981 gctcgagctc gtcggactgg caggattggg cggccgcatg ccgtcgcagt tgtcgggcgg
    14041 ccagcagcag cgcgtggcgt tggcgcgcgc gctggcgcac cggcccgatg tgctgctgct
    14101 ggacgagccg ttcggcgcgc tggacgccaa gatccgcgtg gagctgcgcc gtgcgctgcg
    14161 agcgatacag gaagagctag gcatgacgac gatcttcgtc acgcacgatc aggaggaggc
    14221 cttcgagctg gccgatcgat tgggcgtgat gaacttcggc cgcctgctcg aagtcgggca
    14281 gcccgaagaa ctgtaccagc gcccgcagac cgagttcgtg gcgacgttcc tcggcacggc
    14341 caatttgctg gtggggcagg cgacgccgac cggcgtgcag ttggggccgg tacactttcc
    14401 actgagtact gtggccaccc ccgccgccga gcagcgcgtg caggtgttgt tccggacgga
    14461 ggatgtggcg ctggccgaat catccgacct gctgggctgc ccacacattg gctgcggcga
    14521 ggtcgagcat accaccttcg ccggctcgtt cgagaagctg aggctgcgac tgccgcccct
    14581 acccggcgtg cggcccatct cgccgccggt gtcgttcggc gacaactcga tcctggtcga
    14641 agcgacgcgc tcgcaagacc tgacgcaccg ctttccgttg acacccggca gcaaggtatg
    14701 ggtcggcgtg cggcgcatcc acgcgctggc gcatccgggt ctgagcttcc tgttcttgac
    14761 cgacggctcg ccgcaggcga aggccgcgct ggacgtcggc ggccaaatcg cccgcatggc
    14821 gcacgcgcgc gtgacgattc tcggtttcgg cctggccgaa gagacgctac aacgccacct
    14881 gcaggaagtg aaggagcagt tgggcagcgg gctggcctcg ctcgacctgc gcgcgaccga
    14941 tgacgacgct gtcgaggctg tcgggcgcga gatcgagcgc cagccgttcg acctgatagt
    15001 gctgggacgc cagctgcggg gcggtgtcga gctggccgag gagctgatgc agtcgggcga
    15061 gcaccacctg ctgttggtgc ccgggccgca gccggcgccg tcgcgcgcgc tggtgtgcgt
    15121 gacgagcggc gagccgggca aacacgacgt gctgttcgcc ggccggctga tccgtcacct
    15181 gggcgcagag gcgacgctgc tgtcggtgat gcggcctggc gacgacacgc tgcgccgcaa
    15241 tcaaaccgag cgcttcctgg ccggcggcgt ccgctcgctg gaagtgctgg gcgtccaggc
    15301 gaagtcgaag attcgattcg gcgcggtgcg cgacgaggtc gttgaggagg taaacgaggg
    15361 ccagtacgac ctgctggtgc tcggcgcgcc gctgcccgat cgcgaaggcc gcatctcact
    15421 gaacgggttg gtgggtcagg tgttgagcgg cgtggcggat cgcgccacgc tgattgtgcg
    15481 atcgagctat acgacggcca agctccccat tcttgtgcca ctcggccgtc tcagtctagt
    15541 tgaggaggat caactgtgaa aaagaggata gtgtctaccc ttgtcgtcct ggcgctgatt
    15601 gggctgctgg cggcgtgcac cgggccggcg gccacgcagc cgtccagcca gggatcgatc
    15661 aaactgatcc tgggcgcata caccacaccg cgcgaggcgt acggcaagct gatccctctc
    15721 tttcaggcgc agtggaagaa gcaagccgga caggatgtga ctttcgaaga gtcttactta
    15781 ggctcgggcg cgcaatcgcg cgccatcgtc gagggcttcg aggccgatgt cgccgctctg
    15841 tccttggaag gtgacattac gcgcatcgaa aaagcgggcc tgatcactca ggactggaag
    15901 agcggtacct acaaaggcat ggtcagcacc tcgatcgtcg tcttcggcgt gcgcaagggc
    15961 aaccctaagg gcatccaaga ttgggccgac ctggctcaac cgggcctgga agtccttaca
    16021 cccgatccga agtcgagcgg cggcgcgcag tggaacatcg tggcgctcta cggcgcggcc
    16081 atccgtggca aaatcagcgg tgtgccggcg ggcgacgaag cagcggcatt ggagtttctg
    16141 aagacggtgc tcaggaatgt gaccgtgttc gataagggtg cgcgcgaaag catcacgaac
    16201 ttcgagaagg gcgtcggcga cgtgatcatt acctacgaga acgagatcct ggtcgggcag
    16261 aaaaatgggc aggattacga aatgatcttc ccgcaatcga ccatcctgat cgagaatccg
    16321 gtagccgtga tcgatacgta tgtcgacaag cacggcacac gcgcggcggc cgaggcattc
    16381 gtcgacttcc tgttcactcg tcaggcgcaa gaaatcttcg ccgaatacgg cctgcggtcg
    16441 gtcgatcccg aagttgccca ggcgacggcc agccagtacc cgacaatcga agacctgttc
    16501 accatccgag actttggcgg ctggtcggag gtggccgaca aattcttcga cgacgacgga
    16561 atctactccg tcgcgatcgc gcaggtgcaa ggacagtaga attcccctcg tccctgctct
    16621 ctcctgtcgc tttgcaacgg gaaagggtgc gggagggggg cgcattgacg catggcaacc
    16681 acgtcgttaa ccgcaactca atcaccgcct cgcatcagcg cgccctgggg ccgctggggc
    16741 gtgcgcggca tcgcgatcag ttatctcgcg cttatgctga tcattccgct tgtggtgatc
    16801 ttccaggatg gtctgcgcga gggtctggcc ggcgtctggg aggcggcgtc gaaaccgatc
    16861 gctgcttctg cgctgaggtt gacgctgtgg acggcagcga tcatggcctt gatcaacgga
    16921 gcgatgggcc tgctgaccgc ctatgtcctg gtgcgctatg actttcgagg taagtcgatc
    16981 ttgaatgcga tcatcgatct gccgctcgcc atcccaacgc tggtcacggg cgtgatgctg
    17041 gtcgtgttgt atgggccgca gagcgcggtc ggaagctggc tggatagcac gctcggtttg
    17101 cgggtgatct tcgcgccgcc cggtatcata ctggcgctgc tgttcatcac cttccccttc
    17161 gtcgtgcgcg ccatccaacc ggtgctgctc gacctggagc gggcgcagga ggaagcggcg
    17221 gcgacactgg gggcgggcgg cctgaccatc ttctggcgca tcgtgttgcc gaggctggcc
    17281 gtgcccatga tcagcggcat gttgttgagc ttcgctcgcg cgatcggtga gttcggatcg
    17341 atcgtgatcg tcgccggcaa catcccgttc aagtcgcaga cggcggcggt atacgtactg
    17401 ggcgaggtcg aatcggagaa ccggctggcg gccagcgcgc tgtcgatcgt catgttgagc
    17461 atcgccttcg ggcttgtcct gttggtcagt cgactgcaaa agcgcgggca caagcgagcg
    17521 gagtcaagtt aagcttatgg cccgggttct gtgggcatct ttccgcgccg gccgcgcgac
    17581 gcggccggga gcatctcgga gcagaatcgc atgacaagtc tggcagtggc cgtgccccaa
    17641 cgccgggccg ggcgatgggg acaacgactt ctcatcggcg tcgtcctggc gtatgtggca
    17701 tttctcatcc tcgcaccgat cgccgcgcta gtcgccggcg cgcttcagga tgggctgggt
    17761 tccgctcttg cagccctggc cgcgccagac ttgctcgcgg ctttcgggca gacattgtcg
    17821 atcgggctga tcgtcgtgtg cgtccacgtg gtctttggca cggctgtcgc ctgggtactg
    17881 gtgagacatc gtttaccagg ccgcaatctg ctcaatggtc tgatcgacct accgttcgcc
    17941 gtgtcgcccg tcgtcgtcgg ctatatgctg ctgctgctgt ttggccggcg cggagtgctg
    18001 gcgccgatcc tacagacgct tgatatccag gtcgcattcg ctgtgccggg tatgatcctc
    18061 gcgacgctgt tcgtgtcgct gcctttcatg atccgcgagc tgatgccgat cctggaagag
    18121 ttcgacatcg agcaggagcg tgcggcggcg acgctcggcg cgagcggctg gcagacgttt
    18181 cggctggtga ctctgccggc gctgcgttgg ggcttcgtct acggcatcgt gctcacgttc
    18241 gcccgtgcgt tgggcgaatt cggcgcggtg ctggtcatcg gcggcggcgt gcaggggcgc
    18301 accgagaccg ctacgttgta catatttcgc gccctcgacg agcgccaata cgtcggcgca
    18361 ttcagcgctg cactggtgct cgggctgtgc tcactgctgc tggtgctcgg cgccgacctc
    18421 attcgcagaa agcagggata gggcgccgat gacctcaccc actcccggca cgcagtgccg
    18481 gaagagggaa ggtgaggtaa tatagtcctg tatggctttg cggaatcctc tgcgtgggct
    18541 gcgcctgagc gcgctgctga tcggcatcaa cgtcgggtta ctgctgctgg cggtggcggg
    18601 tgtcgccgcc gtcgcggtga gtctgttgca gcggctggcc gacgagcagg cgctggcgcg
    18661 cgtggcgcag gccggggcga gcgcgcagaa cgcgatcgag cgcgcgggcg acgagatcct
    18721 cagggacgcg cagctcctcg ccgagcgtcc cactctactg cgcctgatcc aatcgagcga
    18781 cgcccaggcg ctgagcgcct tcctcagcca gttccagttg acgagccagc tcgacgcgac
    18841 ggccatcatc gtgagcgatc gggtcttcgc cagcagcggc gcggcgcttc cctgggatgc
    18901 gctgtggccg gagcaccaag acgagagcgg ctattatttc catcgtcaag ccgaaggcgc
    18961 tctgctgatc atgggcgcgc aggtggaggt tccatcgctg accggagcta cggttctggc
    19021 cgcaaaactg ctggatgaat ccttcgcggc gcagatcgca agtgaggtgg gcctgaccgt
    19081 cacgatcctt gagcgcgaat cggccctggc ggacgtcgat ctgcgacaac tgcgatccgc
    19141 cgcgaccggg cagaccgtca ccgcgcgccg cgacgatctg ggcgtgtacc tggcggtctc
    19201 cccgctgcgc gcgccctccg gccagatcgc cggcttcgtc gaaacggtgc tgccgtccac
    19261 tctcctcgcc gactcgctgc gccgcctgat cgaaacgctg gtcgcgctga ccctgggtgt
    19321 cgcggcgctg gcggcgttgg tcagctttct cttcggccgc tggctcgttc atcccttgct
    19381 caggctcaga aacgcggcgg cgcgcatcgg ccacggcgac ctggcgacgc cggtcgtcgt
    19441 cgtctccggc ggcgagatcg gcacgctcgc cgtgacactg gaagacatgc gccgccgcct
    19501 gctgcagctc accgccgatc tcaagcgcca gcaggccgag tcgaatgcca tcgtcaccag
    19561 catcatcgag ggcgtgttca ccgtcgatcg cgagcggcgc atccgctacc tgaacccgca
    19621 ggcggcggtg ctgctcggcg tcaacgccga agaagcgatt ggacaattct gcggcaatgt
    19681 gctaaaccct caggggccgg atcaggtgag gccgtgcgaa gagcagtgcc cgatcgtcca
    19741 tgcgcgattc cgggccggcg cgagtgcgac cgagcatctg cttctcaggc acggcgcacg
    19801 gcgcacagtc gtcatcacca gcgcgccgcc gtcggagaac cggcaggtgc aggtcattcg
    19861 tgacgagacc gaggtggaag cgacgcgccg cctgcgcgat gcggtgctgg cgaacatttc
    19921 gcacgaattc cgcacaccgc tctcggcgca gttggcgtcg atcgagctgc tgctcgatca
    19981 actgccggac ctatccactg agcagatcgg cactttgatc ctgtcgctgc agcgcggcac
    20041 gctgcggctg acgcagttga tcgacaacct gctggagagc gtgcgcatcg aggcgggcga
    20101 gcacgccatc cgccgccagc cggtgctgct cgacgaggtg atcgagcagg ccctggagtt
    20161 cacgcaaccc ctgctggcgc agcgcgagca ggaggtggag atcgagttgc cctatcccat
    20221 accggcgatc tcgggcgacg cgacgcggct gacgcaggtg ttcgtcaatc tgctggcaaa
    20281 cgcgaacaag ttcgcgccgg ccggctcgac gatccgcatc ggtggcgcgg tcgagccgac
    20341 caccatccgc gtgtgggtcg aggatcaagg ccccggcatt ccgccacagg ccgggcagtc
    20401 gctcttcacg cgcttcgtgc gctcgacggg agaagagccg gagcagggtg gcgtagggct
    20461 ggggctgtgg atcgtgaaat cgatcgtcga gcgccacggc gggcacgtcg aggcattcag
    20521 cgattcgacc ggcacgcgaa tgcgcatgac tttgccgcga gggaatgagt cgtgaagata
    20581 ctggcggtcg acgacgaccg cgagctgctc aacctgatta gtttcgcgct gcgccagagc
    20641 ggttacctgg tgatcgaagc ctcgaacggc gatgcggcgt tgagcgcgtt cgagcgcgag
    20701 cagcccgacc tggtgatcct cgacgtcaac ctgccgggcc taagcggttt cgacgtctgc
    20761 cgccgcatgc gcgagcaggc cagcacgccg atcatgatgc tcaccgtgcg cagcagcgaa
    20821 gaggacgagg tgaaagggct ggacctgggc gcagacgact acctgaccaa gccgttcagc
    20881 ccgcgaacac tcctggcccg tgtgcgcgca ctcttgcgcc gcgccgatgc cgagcgacct
    20941 gcgccggcgc tgggcgctgg cgacgtcgtg ctggatgtcg agatgcagac cgtgagtgtg
    21001 cgcggcggcg caccggtccg gctgacgaac ctggagtttc gattgcttca atatctgctc
    21061 gccaatgccg ggcgcgcgct gtccg
//
LOCUS       DBNHXB010000031        12148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1852927, whole genome shotgun sequence.
ACCESSION   DBNHXB010000031 DBNHXB010000000
VERSION     DBNHXB010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 12148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12148
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1852927"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="AC162G_01275"
     CDS             complement(<1..1330)
                     /locus_tag="AC162G_01275"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HIR5074478.1"
                     /translation="MTTISTAENQPKKASSVRHTRAIQRDRTKRPPAAPPDDQVTARL
                     TEIVHPATLNQVGYYHQLGLRERTLTLPVMVALVLSLIWRQISGVSELVRLVRTEVLL
                     WVPPLKISQQSLSERLNTLPSELFLRVLVSLLPFLQSRWSQRQRPLPSVIAWAQARYT
                     QLAMCDGSTLDALIRKVGLLRDLPANPLAGRMTALLDLCSRLPFHVWYDEDAQAHDQR
                     FWPKILAFLKAGALLIFDLGYTNFTVFTQLTAAQITFITRAKRNLAYQLERALRRTAS
                     VHDLVVWIGSGEDRQLVRLIAVLYRGKWYRYLTNELDPDRLPTEYVVALYWQRWRIED
                     AYAIVKRLLGLAYFWCGSQNAVELQVWATWLLYAVLVDLTDAVAEALQQPFAALSLEM
                     VYRSLYYFTQAYHRGETADIVIYLAANARWLGLIKRKRRRKPLPLELSPLT"
     gene            complement(1349..1741)
                     /locus_tag="AC162G_01280"
     CDS             complement(1349..1741)
                     /locus_tag="AC162G_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HIR5074479.1"
                     /translation="MVEVTICLLVKGHPPTQLLLGWKKTGLGQGKYAGLGGKVEAGET
                     REVTAIRELHEEAGVIVSLDDLEYLGHLTFLFPSKPEWDELAHVFLVRKWDGEPVETR
                     EMKPTWFALAHWSQVNDFWLLSSPALNA"
     gene            complement(1761..2519)
                     /gene="ribA"
                     /locus_tag="AC162G_01285"
     CDS             complement(1761..2519)
                     /gene="ribA"
                     /locus_tag="AC162G_01285"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HIR5074480.1"
                     /translation="MPLTHMQIKELLEENRTHDCDGYGPDKVCVRIAAIADLPSRFGD
                     FHVVAFYNNRDNKEHAAFVHGDVCGRENVPVRVHSECLTGDAIGSLRCDCRDQLEAAL
                     KQIGQMDKGILLYMRQEGRGIGLTNKIRAYGLQDHGYDTVDANIALGFRDDERDYAIA
                     AHMLESLKVKSIRLMTNNPRKIDDLTRHGVVVTERIPLIIPPNPHNEFYLQTKAERSG
                     HLIDLRGKEHLLEQGDRPIVEGMTPEQVTALPQE"
     gene            complement(2558..3247)
                     /locus_tag="AC162G_01290"
                     /pseudo
     CDS             complement(2558..3247)
                     /locus_tag="AC162G_01290"
                     /inference="COORDINATES: protein motif:HMM:NF013989.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
     gene            complement(3491..3739)
                     /locus_tag="AC162G_01295"
     CDS             complement(3491..3739)
                     /locus_tag="AC162G_01295"
                     /inference="COORDINATES: protein motif:HMM:NF046758.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FitA-like ribbon-helix-helix domain-containing
                     protein"
                     /protein_id="HIR5074481.1"
                     /translation="MPILHIRNVPDDLYARIRRLASARSRSISAQVVQLLDQAVQAEE
                     ARQSQARILAGIRRRRVSYTKTPSTPDSVALLREDRER"
     gene            3985..5016
                     /locus_tag="AC162G_01300"
     CDS             3985..5016
                     /locus_tag="AC162G_01300"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR5074482.1"
                     /translation="MANVSRRDFLRFAGAALATPLIGPDPPDPAGSARPIGLGRVTDF
                     VTWAYVDPKPDAAKVKSFRRDTVLKIYDSVTDETLLPHNPVWNLTPFGWVHAWALQPV
                     AYQPNRPVRDVPEAGFWAQVTVPYVAMRSRPDDEARLLYRLYYSTVHLVIAHSEDARG
                     RSWYQLRDDQQPNRLEYVRAEGLRRIRPEEMTPISPDAEDKVIEVSIKEQMVYAYENG
                     SLVFSTQCATGAAFTVEGLGVVNFSTPYGEHSVVRKRPSRHMIGFLGRSDAYDLPGVP
                     FCTYFTTAGAAIHGAYWHNDYGHPRSHGCVNVLPEAAQWVYRWTMPAAAYEDALLEVE
                     SDGSPIIVS"
     gene            5017..5655
                     /locus_tag="AC162G_01305"
     CDS             5017..5655
                     /locus_tag="AC162G_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR5074483.1"
                     /translation="MPTYLIILSPALLHREAWRALLTGQPDVIVSGALAEPAHIATLI
                     QPHQPATLLIDLPDPQPDLARHIRSSAPEVGVLFLAQSYDLAEIVPCLQAGATGFVSR
                     DESTGDLVRAIIAAGRGEIVLPPSIAARVLIALARGEAVATNPTEALSEREIEVVRLL
                     GQGLTNKDIAQTLILSVRTVEAHLRNIYGKLGVRSRTEAALWAVRHGYGPEQ"
     gene            5736..6353
                     /locus_tag="AC162G_01310"
     CDS             5736..6353
                     /locus_tag="AC162G_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074484.1"
                     /translation="MNAQPRAPTPTPIAVLGTLAEFHREPIPYDLNALVQLVTDLRPD
                     LLCLDITPEQWRRRDFSNLPPEYRDALLPLAHQTDIVVVPIGEDLSLQEPTAPGWRGR
                     VIGVLRRWLAHLQRTAPGPAAINEGWRHHAADLLYSLIAWLAGSDAQRAWRAHTAHLT
                     RQVLEVARRDPGSRIMVVVNVRHCHHIRPALRQSPNIRVVKYSQL"
     gene            complement(6396..7001)
                     /locus_tag="AC162G_01315"
     CDS             complement(6396..7001)
                     /locus_tag="AC162G_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004951297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5074485.1"
                     /translation="MIRVLLAEDQAMVRGALAALLRLEGDIEVVAEAVNGNEVLPAAL
                     KTQPDVALLDIEMPGADGLSAAQALHERLPSCRIMILTTFGRSGYLRQAMESGAVGFL
                     LKDAPAVELATAIRRVMRGERVVDPELALAALSEGDNPLTRREREVLKAALDGASIAE
                     IAARLYLSEGTVRNHFSIAIQKMGAHNRIEAARLAEAKGWL"
     gene            complement(6998..8266)
                     /locus_tag="AC162G_01320"
     CDS             complement(6998..8266)
                     /locus_tag="AC162G_01320"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5074486.1"
                     /translation="MDIEQVAEERSQDQPAELNSGTPRPREVVASSGISIRLWRLYAQ
                     AWLVCLLFPIVFLAQTPLAAVRLLIAVAGLVIFAVTYTWFMWPHPLESEAHARPGSRP
                     SLMLFAGLVALTLFLSMAYGSAFLWLFVGVSAVSGVALSARSAFVTVLVLTLLTLGVG
                     VAISGGIARTDWLHILPLVLLVRGLGLDIVGLVRLSDALRELHATRGELARQAVMEER
                     LRLARDLHDLLGHTLSMITLKSELAGLLIEKEPARAAQEIHEVERVARQALREVREAV
                     AGYRQPTLRSELNGAQQMLEAAGITCTIENSVRALPPATAAVLAWTVREGVTNVIRHS
                     RARQCTIRVSSGNGKASAEVINDGCREQERDLGRTGASSGLSGLTERVIAQGGQIEAG
                     PLPSESNPSFRLWVELPIRHGEAVEEEQPQ"
     gene            complement(8346..8912)
                     /locus_tag="AC162G_01325"
     CDS             complement(8346..8912)
                     /locus_tag="AC162G_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074487.1"
                     /translation="MNLYSLMLFLHVSGDIGIFIGIGVQLLSLAALRRAKSVEQVRAL
                     AGLISLSDPISVISALVTIAAGLYMALTVWGLQTGWIAVALASLIVLLPPLIAGVVEP
                     RMRAIVTMAKEVPDGPLPATLDTRIHDPVLGTALYTVAALVLGIVFLMTNKPSLTASI
                     LAMVVALALGLASGLPLWRTAHTIRTRL"
     gene            complement(8977..11082)
                     /locus_tag="AC162G_01330"
     CDS             complement(8977..11082)
                     /locus_tag="AC162G_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR5074488.1"
                     /translation="MNPLLLPLTEVDRGFLPLVGGKGANLGEMARARLPVPPSFCVTT
                     VAYTRVAAQAQIESLAVELAQMPARSIERLAAQASRIRDLIEATPIPADIADTISAAY
                     RQLGDGVPVAVLSSATAEDLPGAAFAGQQDTYLNVVGAVALLDAVRRCWASLWTDRAT
                     AYRERQEIDQRTVKIAVIVQRMVPAEVAGVLFTIFAEMFPVRPYPLDQTTWVHAISAA
                     AVEPIFGLIGIAVPSIEQMFIEEDGVVVRFSGKVVVPPTPAILLAPIRLLRLARRYDP
                     THWSADPLLAEARSRARALEMQDLGTLSWQDLLATVRDALALAQPLAGEMRRRYLPRA
                     LLAAGLLRVALALLRRGHRFGTLLSGVESTTLEANRALEALAARVRPDPTLAETFARR
                     EAGELWTALEQQPSSRAFLSELQAFLGRYGHREVVLSTVLQPTWKDAPELALGIIKGL
                     ALTEPRHETERPAWEVARDNVLAHPVLRFSPLRAAILTLLAAARSLWQIRENTHYDAT
                     RILPILRRTLLELGRRLVTVDVIDAPEDVFHLKLDELEGIGGTWPSAPERADELRAIL
                     LRRKERRAALEGTPLVDPRLYRQREPSSDALLRGTSGSPGVAEGPVRVIRDGSEFGKL
                     RAGEVLVAPYTNPAWTPLFQRAAAVVVDSGGAGSHAAIVAREYGIPAVMGTIDGTRRL
                     ADGQHVRVDGDHGRVTLLD"
     gene            complement(11401..11985)
                     /locus_tag="AC162G_01335"
     CDS             complement(11401..11985)
                     /locus_tag="AC162G_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5074489.1"
                     /translation="MSHSTRIEAPDVSTTFEAYYDRIHRYILSVIRDPAEADDLTQET
                     FLRAYRRYDTLHDAGALSTWLYRIATNVCLDQLRQRARRAPLESETDPDDVDLADPDI
                     PSLQRTVERNEMSTCVQQYLENLPDSYRAVILLHDLHGLTGPAIAGTLGVPLATVKIR
                     LHRARRKLQAALEAGCAFSHDERDVLVCEPNADP"
BASE COUNT         2306 a         3850 c         3752 g         2240 t
ORIGIN      
        1 ttgtcaaggg tgagagttcg agcggcaacg gcttgcggcg gcgttttcgc ttgatcaggc
       61 cgagccacct ggcgttggcc gcgagataga tgacgatatc ggcggtttcg ccacgatgat
      121 aggcttgagt gaagtaatac aggctgcggt agaccatctc cagcgagagc gcggcaaagg
      181 gttgctggag ggcttcggcc accgcgtccg tgaggtcaac caagacggcg tacaacagcc
      241 acgtggccca gacttgcagt tcgacggcgt tctgggagcc gcaccagaag taggccaggc
      301 ccagcaagcg cttgacgatg gcgtaggcat cttcaatccg ccagcgctgc cagtacagtg
      361 ccacgacata ctcggtcgga aggcggtcgg ggtcgagttc gttggtgagg tagcgatacc
      421 acttgccgcg gtacagcacc gcaatcagac ggacgagttg gcggtcctcg cccgacccaa
      481 tccagaccac caggtcatgc acgctagccg tgcgacgcag cgcgcgctcc agttgatagg
      541 ccaggttgcg cttggcccgg gtgatgaagg tgatctgcgc ggcggtcaac tgggtgaaga
      601 ccgtgaagtt ggtgtagccc aaatcaaaga tgagcaaggc gccggccttg agaaaagcca
      661 ggattttcgg ccaaaaacgc tggtcatggg cctgggcgtc ctcatcgtac caaacgtgaa
      721 agggcagccg cgaacacaga tccagcaggg ccgtcatgcg cccggccaag gggttcgcgg
      781 gcaggtcgcg caacaggccg actttgcgga tgagcgcatc cagggtcgac ccatcacaca
      841 tggccagttg cgtgtaacgg gcttgcgccc aagcgatcac tgacggcagt gggcgttgcc
      901 gctgggacca gcgactctgg aggaatggca gcagactgac caacacgcgc aagaacaatt
      961 ccgagggcaa ggtattgagg cgttccgata acgattgctg gctaatcttc aggggcggca
     1021 cccacaggag cacttcggta cgcaccaggc gaaccaactc gctcacgccg ctgatctgcc
     1081 gccaaatcag gctcagcacc aaggcgacca tcaccggcag cgtgagcgtc cgctcgcgca
     1141 gccccaactg atggtagtaa cccacttggt tcagtgtggc ggggtgaacg atctcggtga
     1201 gccgggccgt gacctgatcg tccggtggcg cggccggggg gcgtttggtg cgatcccgct
     1261 gaatcgcccg cgtatgtcgt actgaactgg ctttcttagg ttggttttcg gccgtgctaa
     1321 tcgtggtcat gcgccaatct taccgcagtt atgcgttcaa ggcagggctt gacaacagcc
     1381 agaaatcgtt aacctgtgac caatgggcta acgcgaacca tgtgggcttc atctccctgg
     1441 tttcgaccgg ctcaccgtcc catttgcgga cgagaaagac gtgggctagt tcatcccact
     1501 ccggcttcga cggaaacagg aaagtgaggt gacccaggta ttcgaggtcg tccagggata
     1561 cgatgacgcc tgcttcttcg tgaagctctc tgatcgcggt aacctctctg gtctcaccgg
     1621 cttctacctt accgcccagg ccggcgtact tgccctgacc gaggccggtc ttcttccagc
     1681 ccaggaggag ctgtgtgggt ggatgacctt tgacgaggag gcaaatggtg acttcgacca
     1741 tagcatattt tccaggggcg tcattcctgc ggcagcgctg tgacctgctc cggcgtcatg
     1801 ccctcgacga tggggcggtc gccctgttcg agcaaatgct ctttgccgcg caggtcgatc
     1861 aggtgccccg atctctcggc tttggtctgg aggtagaact cgttgtgcgg attcggcgga
     1921 atgatgagcg ggatgcgctc ggtgacgacg acgccgtgcc gcgtcaggtc gtcgatcttg
     1981 cgcgggttgt tcgtcatcag ccgaatcgac ttgaccttca gcgattccag catgtgcgcg
     2041 gcgatcgcgt agtcccgctc gtcgtcgcgg aagccgagcg cgatgttcgc gtcgacggtg
     2101 tcgtaaccgt gatcctgcaa gccgtaggcg cggatcttgt tcgtcaagcc gatgccgcgc
     2161 ccttcctggc gcatgtacag cagaatgcct ttgtccatct gcccgatctg cttgagcgcc
     2221 gcctccaact gatcgcggca gtcgcagcgc agcgagccga tcgcgtcgcc cgtcaggcac
     2281 tcggaatgca cgcgcacagg gacgttctcg cggccgcaga cgtcgccgtg aacgaaggcg
     2341 gcgtgctcct tattgtcgcg attgttgtag aaggcgacga cgtggaaatc gccgaagcgc
     2401 gaaggcaggt cggcgatcgc cgcgatgcgg acgcacacct tgtccgggcc ataaccgtcg
     2461 caatcgtgcg tccggttttc ctcgagcagc tccttgatct gcatgtgagt gagtggcatg
     2521 gtttctcttc ttttcctgtc cgtgtagaat tctttaacta gagcacccgg taatgtacca
     2581 cgaccccgcc atcctccagt tgctcgacgt caagctcctg cagccggagg gcttcttcgc
     2641 gcgggaagcc cggcccgccg gcgagcgtgg gcgcgtccgc tccgccgaag atgagcggcg
     2701 cgacgtagat gatgacttcg tcgacgagcc gctggtagag cagcgccgca ttcagcgtgc
     2761 tgcccccttc gacgagcagc cgggccacgc cgtgcgttcg cagcgagcgc atcatcgccg
     2821 gcagatcgac ctgcctctca cccatgacga acacgctgac gccctgcgcg cgcagacgct
     2881 cgatttgcgc caggccggtc tgctgcgccg tgtacaggaa tacacgcgcc gggccggcgg
     2941 tcacgaactg gctgtccgat cgcagcccgg cagcgtcgga gaggacgccg actttgatcg
     3001 gattctcgtc caggtcgcgc gcgcggcgcc cggcgcgcag tgccgcggac ttcacggtct
     3061 attttggatc gtcgcccagc agcgtgcgcc cgccgaccat caccgcatcg ctctcggcgc
     3121 gcagccgatc gacgcgctct cggtcgcgcg gcgacgagat ggccgcgccg cgccgcgcgg
     3181 ccgtgtcgat cttcccgtcg gcggtcatcg ctacgttcag gatgacgaat gggcgctcgc
     3241 cgatcatccg atctcccgcg agcggatcac cgcgaaagtg ttatccgtct catcccacgc
     3301 caagcggaat gtcaaaatcc cccggccaac gcacatcata cgaagtagtc gccaacaagc
     3361 gcgccagttt ttcatccgcc gtcacgcagg gcacgcccaa atgctgtgcc agcgccacgt
     3421 agcaagcatc atacgatgaa tagcttgatc ccaacactgg tgtcgcccac acactcaagc
     3481 cgggccgcca tcatcgctca cgatcctcgc gcagcagcgc cacgctgtct ggcgtggagg
     3541 gcgtcttggt atacgaaacg cggcgacggc ggattccagc caagattctg gcctgcgact
     3601 gcctggcctc ttcggcctgt accgcctgat ccaacagttg aacaacctgc gcgctgatgg
     3661 agcggctcct ggcgctggca agtcgccgga tacgtgcata caagtcgtcg ggcacgtttc
     3721 gaatatgaag aatgggcact gcagtttccc cctcgtggaa tgattgaagt atatcactgg
     3781 tggttgcgtt atgcatccgc ctgactcgat cctgcgcctc gacctgtcgc atagacctga
     3841 ttccgcttta gtttacgtcg gattcaagac ttcatccgga aaccgcctaa aggctcgatc
     3901 ccggagcggc gtaaacgcaa tttgataact tgcggcagcg tgatatactt tatccccagc
     3961 agacaaacat gaggagattg actcatggcg aatgtgagcc gacgcgactt tctccggttt
     4021 gccggtgcag ccttagcgac gccgctgatc ggacccgatc cgcccgatcc ggcaggatcg
     4081 gcccgcccca tcggcctcgg ccgcgtgacc gatttcgtaa cctgggccta cgtggatccg
     4141 aagccggacg cggctaaggt gaaatccttc cggcgtgaca ccgtgttgaa gatttacgat
     4201 tcggtcaccg acgaaaccct cctgccgcac aacccggtct ggaatctgac gccgttcggc
     4261 tgggtccatg cctgggcgct gcagccggtg gcgtatcaac cgaatcgccc ggtgcgcgat
     4321 gtgccggagg ccggtttctg ggcgcaggtc accgtgccgt acgtcgccat gcggtcaagg
     4381 ccggacgacg aagcgcgctt gctctaccgg ttatactaca gcaccgttca tttggtcatt
     4441 gcccacagcg aagacgcacg gggtcgatcg tggtatcaac tgcgcgacga ccagcagccg
     4501 aatcggttgg agtacgtgcg ggccgagggt ctgcgtcgca tccggcccga agaaatgacg
     4561 ccgatctcac ccgacgcgga agacaaagtg attgaagtca gcatcaagga acagatggtg
     4621 tatgcgtacg agaacgggtc actcgtgttt tcgacgcagt gcgcgacggg cgcggccttc
     4681 accgtcgaag gtctgggcgt ggtgaacttc agcacgcctt acggcgagca ctcagtcgtt
     4741 cgcaaacgtc cgtcgcgcca catgatcggc ttcctgggcc gctccgacgc ctacgacctg
     4801 ccgggcgtgc cgttctgcac ttacttcacc acagccggcg cagcgatcca tggcgcctac
     4861 tggcacaacg actacggcca tccgcgcagc cacggctgcg tcaacgtgct gcccgaggcg
     4921 gcgcagtggg tgtatcgctg gacgatgccg gcggcagcat acgaagatgc gctgctggaa
     4981 gtggaaagcg acggatcgcc cattatcgtc tcgtagatgc cgacttacct tatcatcctg
     5041 tctccggcct tgctgcatcg cgaggcgtgg cgggcactgc tgaccggcca gcccgacgtg
     5101 atcgtctccg gtgcgctcgc cgaaccggct cacattgcca ctctgataca accccatcag
     5161 ccggcaacac tcctgatcga tctgcccgat ccccagcccg atctggcccg tcacattcga
     5221 tcgtccgcgc cggaggtcgg agtgctgttt ctcgcgcagt cttacgatct ggccgagatc
     5281 gttccatgct tgcaggccgg tgcgacgggt ttcgtgtcgc gagatgagtc gaccggcgat
     5341 cttgtgcgtg cgatcatcgc cgccgggcgc ggcgagatcg tcctgccgcc gtcgattgcc
     5401 gcacgcgtgc tgatcgctct cgcgcgagga gaagccgtcg cgacgaatcc gacggaagcg
     5461 ctgtcggaac gcgagatcga ggtcgtgcgc ctgctcggcc agggcctcac caacaaagac
     5521 atcgctcaga cacttattct cagcgtgcgc acggtcgaag cgcacctgcg caacatctac
     5581 ggcaagttgg gcgtccgctc gcgcaccgag gcggccctgt gggccgtcag gcacggctat
     5641 gggccggagc aataggtgat ttcaccgatg ccgattcagg cgattctgcc atagtctcaa
     5701 gaagtgtgcg cacatatact gatggcacaa aatagatgaa cgcacagcct cgcgctccaa
     5761 cacccacacc gatcgccgtg ctcggcacac tggccgaatt tcaccgcgag ccgatcccct
     5821 atgacctgaa cgcgctggtg cagttggtga ccgatctgcg ccctgacttg ttgtgcctgg
     5881 acatcacacc cgagcagtgg cggcggcgcg acttcagcaa cctgccgccc gaataccggg
     5941 acgcgttact gccattggcg catcaaaccg acatcgtcgt cgtgccgatc ggcgaagacc
     6001 tctctttaca agagccaaca gcaccgggct ggcggggaag ggttatcggc gtcctcagac
     6061 gctggttggc acacttgcag cgcaccgcac ccggtccggc ggcgatcaac gaaggctggc
     6121 gtcatcacgc cgccgacttg ctgtacagcc tgatcgcatg gctggccgga agcgacgcgc
     6181 agcgcgcctg gcgcgcgcac acggcacacc tcacccggca agtgctcgaa gtcgcccggc
     6241 gcgaccccgg cagccggatc atggttgtcg tcaacgtacg gcattgtcac catattcgtc
     6301 cggccctgag gcagtctccg aacattcgcg tcgtgaagta ttcgcaactg tgaaagcccg
     6361 ctgatgctct ggggtttggc gacagccatt cacttctaca accatccctt cgcttcagcc
     6421 agccgtgctg cttcgatccg attgtgagcg cccattttct gtatggctat ggagaaatga
     6481 ttgcgtacgg ttccctcaga cagatacagc cgggcggcga tctccgcgat gctggcgccg
     6541 tccaacgcag ccttcagcac ttcccgctcc cgccgggtga gcgggttgtc gccctcgctc
     6601 aatgctgcaa gggcgagctc aggatcgacg acgcgctcgc cacgcatcac acgacgaatc
     6661 gcagttgcca actcaactgc tggagcatcc ttgagcagga acccaacggc gccgctctcc
     6721 atcgcctggc gcagatagcc gcttcgccca aaggtcgtga ggatcataat tcggcaggat
     6781 ggcaggcgct cgtgtagcgc ttgagccgcg ctcagcccat cagcaccagg catctcaata
     6841 tccagcagcg ccacatcggg ctgagtcttc aacgctgccg gtagcacctc gttcccgttt
     6901 acagcctcag caacaacctc aatatcgcct tccaggcgta gcagcgccgc cagtgcgcca
     6961 cgcaccatcg cctgatcttc ggccagcagt acccggatca ttgtggctgc tcctcttcaa
     7021 ccgcctcgcc gtgtcggatc ggcagctcaa cccataagcg aaaactcgga ttgctttcgg
     7081 aaggcagcgg gccggcttca atctggcctc cttgtgcgat gacgcgctcg gtgagaccgg
     7141 agaggccgct gctggctccc gtgcgtccga ggtcgcgctc ctgctctcga cagccatcgt
     7201 tgatcacctc ggcgctggct tttccgttcc cactgctcac ccggatagta cattgccgcg
     7261 cgcggctgtg ccggatcacg ttggtcacgc cttcgcgcac cgtccaggcc aacacggcag
     7321 cggtggccgg cggcaatgcc ctgacgctat tctcgatcgt acaagtgatc ccggctgcct
     7381 ccagcatttg ctgcgcgcca ttcaattcgc tgcgcagtgt tggctgtcga tacccggcga
     7441 cggcctcgcg cacctcgcgc agcgcctgac gcgctacgcg ctctacttcg tggatttctt
     7501 gcgccgcccg cgcaggctcc ttctcgatta agagccccgc gagctcgctc ttcagagtga
     7561 tcatggacag agtatgcccg agcaaatcgt gcagatcgcg ggccagccgc agtcgttctt
     7621 ccatcaccgc ctggcgagcc agttcgcccc gcgtggcgtg caattcgcgc agcgcatccg
     7681 ataagcggac caggccaacg atatccaggc ccagtccccg gaccagcagc acgagcggga
     7741 ggatgtgaag ccaatcggtc ctggcgatgc cgccactgat cgccacgcca acgccaagcg
     7801 tgagaagcgt cagcaccagg acggtcacga aagcgcttcg cgccgagagc gcgaccccgg
     7861 agacggcgct gacgccgacg aaaagccaaa gaaaggcgct cccatacgcc atgctgagaa
     7921 agagcgtcag cgccacaagg ccggcgaaca gcatgagaga cggacgggag ccaggtcggg
     7981 cgtgcgcctc actctccaag gggtgaggcc acataaacca ggtataggtg accgcgaaga
     8041 tcaccagccc ggccacggcg atgagcaggc gcaccgctgc taacggcgtc tgcgccagaa
     8101 agacgatggg gaacagcagg cacacgagcc acgcctgcgc atatagccgc cagaggcgga
     8161 tggaaatccc gctggatgcg acaacctccc gtggccttgg tgtgccgctg ttgagttctg
     8221 cgggttgatc ctggcttcgt tcctctgcca cctgttctat gtccatacct tcgacaacct
     8281 tatctgaatc accgcccgcc ttacggagtg attatactct tccgtccgcg agtgagccaa
     8341 ggactttaca accgtgtgcg gatcgtgtga gctgtgcgcc agagcggcag acccgacgcc
     8401 aaaccgagag ccagcgcgac gaccatcgcg aggatagagg cagtgagcga gggcttattg
     8461 gtcatcagga agacaatacc gagcacaagg gcagccaccg tgtagagcgc agtgcccaag
     8521 accggatcat ggatgcgcgt gtcgagcgtt gcagggagcg ggccgtccgg gacttcttta
     8581 gccatcgtca cgatagcgcg cattcgtggt tctacgacac ccgcgatgag aggtggcagg
     8641 agcacgatca ggctagccag cgcgacagca atccagccgg tctgcaagcc ccagaccgtt
     8701 agtgccatgt acagcccggc cgcgatggtg accagcgcgc tgatcacgga aatcggatcg
     8761 gatagggaga tcagccctgc gagcgcacgc acctgctcga cggacttcgc ccgccggagc
     8821 gctgcgagac tgagcagttg gacgccaatg ccgataaaaa tgccaatgtc accactcacg
     8881 tgtagaaaca acatgaggct atacaggttc atcaaacgct ctccttttgc atgaagcgat
     8941 acgaatcatc cgtgtcatct gtgtatcgaa catctttcaa tccaaaaggg tgacacgccc
     9001 atggtcaccg tccactcgca cgtgctggcc gtctgccagc cgccgggtgc cgtcaatggt
     9061 tcccatcaca gcggggatgc cgtactcgcg cgccacaatg gccgcgtgtg acccggcgcc
     9121 gccgctgtcc accacaaccg cggcggcccg ctggaagagt ggcgtccagg ctgggttcgt
     9181 gtacggggcg accagcactt cgcccgcgcg cagcttgccg aactccgagc cgtcgcggat
     9241 gacgcgcacc ggtccttcgg cgaccccggg actgccggac gttccgcgta gcagtgcatc
     9301 gctgctgggc tcgcgctggc ggtagagtcg gggatccacc agcggtgttc cttcgagcgc
     9361 ggcacgccgt tccttgcgcc gcagcaagat ggctcgcagc tcatcggcgc gttccggcgc
     9421 tgacggccac gtcccgccaa taccttcaag ctcgtctagc ttcagatgga agacatcctc
     9481 aggagcatcg atcacatcga cggtgacgag gcgccggccc agctcaagca gcgtgcgccg
     9541 gagtatgggc aagatgcgcg tcgcgtcgta gtgggtattc tctcgaatct gccagaggct
     9601 gcgcgctgcg gcgaggagtg tgagaatggc ggcgcggagg ggtgagaatc tcagcacggg
     9661 atgagccagc acgttgtccc gcgctacctc ccatgccggt cgttcggtct cgtgccgtgg
     9721 ctctgtcaag gcaagtccct tgataatacc gagcgccaac tccggtgcat ctttccaggt
     9781 cggctgcaac acggtgctga gtaccacctc gcggtggccg tagcgcccca ggaacgcttg
     9841 cagctcggac aggaaggccc ggctcgatgg ctgttgctcc agcgcggtcc acaattcacc
     9901 ggcctcgcga cgtgcgaagg tctcggctag agttgggtcg ggacggacgc gcgccgccag
     9961 cgcctcaagt gcgcggttcg cctccagcgt cgtgctctcg acacctgaga ggagcgtgcc
    10021 aaaacggtgg ccacgcctca gcagcgcgag tgcgactcgg agaagcccgg cggcgagtaa
    10081 agctcgcggc aaataccggc gacgcatctc cccggcgagc ggctgcgcga gcgccagcgc
    10141 atcccgaacg gtagccagca gatcctgcca cgagagagtt cccaagtcct gcatttccag
    10201 agcgcgagcg cgcgagcgtg cctcggccag cagcgggtcg gcgctccagt gggttgggtc
    10261 gtagcggcgg gcaagccgta gcagccgtat cggcgcgaga aggatcgccg gggtcggagg
    10321 gacgacaact ttcccgctga accggacgac gacgccgtcc tcctcgatga acatctgctc
    10381 gatggacggg accgcaattc caatcagccc gaagatgggt tcgacggcag cagccgagat
    10441 cgcgtgcacc cacgtcgtct ggtcgagtgg gtagggccga accgggaaca tctcggcgaa
    10501 tatggtaaac agcacgcccg ctacctccgc cggaaccata cgttgcacga tgacagcgat
    10561 cttcaccgtg cgctggtcga tttcctggcg ctcacggtag gcggtggcgc gatcggtcca
    10621 cagcgaggcc caacagcgtc gcacggcgtc cagcagtgcc actgctccga caacgttgag
    10681 gtaggtgtcc tgctggccgg cgaatgccgc ccctggcaag tcttcggctg tcgcactcga
    10741 aagcacggcc accggtacgc catcacccaa ttggcggtac gcggcgctga tggtgtccgc
    10801 gatatcggcc gggatggggg tggcttcaat taagtcgcga atgcggctcg cctgcgcagc
    10861 gagtcgctca atcgagcggg ctggcatttg cgccagctca acggcaagcg attcgatctg
    10921 cgcctgcgcg gcaacgcgcg tatacgcgac agtggtgacg cagaaactgg gcggcacggg
    10981 caaacgggcg cgcgccatct cgccgaggtt agcgcccttg ccgcccacca gtgggagaaa
    11041 gcctcggtca acttcagtga gagggaggag gagcgggttc atttcgtttt agagtgtagc
    11101 ggggaagtga gatgaaggga agtgaagcct gtcttgatta tgagtacgac aaataggatg
    11161 ataagggacg tgacaattgt catggggaga tgcgggtagc gcgctgcacg acttccgcat
    11221 cagacgtcgc acacgcggtg agtcgcctgc gcacgatgaa caacgtgcaa ttgccgaaca
    11281 tgatgcccgc aacaaacgag acgcctccag tcgaaggcga caccgactgg agacgtcttt
    11341 aatgacgagg gttttcgagt ccgaggcttc cgatctcacc gcctatgagg cggtaagcaa
    11401 ttacggatca gcgtttggct cgcacaccag cacgtcgcgc tcgtcgtgcg agaacgcgca
    11461 gccggcttcc agcgcggcct gcaatttgcg gcgggcacgg tgcaggcgga tcttcacggt
    11521 ggccagcggc acgccgaggg tccccgcgat cgctggcccg gtcagcccat gcagatcgtg
    11581 tagcaggatc actgcgcggt aactatccgg caaattttcg aggtattgct gcacgcaggt
    11641 gctcatctcg tttcgttcta cggttcgttg cagggagggt atgtccggat cggctaggtc
    11701 cacgtcgtcg gggtcggtct cagactccag cggtgcccgg cgggcgcgct ggcgtagctg
    11761 gtcgagacag acgttggtgg caatgcggta caaccaggtg ctcagtgccc cggcgtcgtg
    11821 cagcgtatcg tagcgacggt acgcacgcag aaacgtctcc tgtgtcaggt cgtccgcctc
    11881 cgccgggtcg cgtatcacac tcagaatgta gcggtgaatc cggtcatagt aggcctcgaa
    11941 agtcgtcgag acgtccggtg cttcgatacg ggtgctgtgg gacatgcaat ccttccttcc
    12001 attcagatga cactcacagc caagaagata cacccaagat gttctgctct gcccatttct
    12061 cacctgccgg gcagactcct ttcactccat ccacgatgac cgaaaaatcc ggcgtcactg
    12121 tcgccattgt tgactccttt cgtctatt
//
LOCUS       DBNHXB010000032         3555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_343363, whole genome shotgun sequence.
ACCESSION   DBNHXB010000032 DBNHXB010000000
VERSION     DBNHXB010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_343363"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1244)
                     /locus_tag="AC162G_01340"
     CDS             complement(120..1244)
                     /locus_tag="AC162G_01340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5074490.1"
                     /translation="MRIGLIVPGFSSDENDWCIPALLHLARALAARHEVHVFALHYPY
                     RRDHYSVFGARVHAIGGANRRGWRLPGVLKAAANEIVLEQRRGRFDVMHAFWVYEPGL
                     IAAWLKRRLGVRVIVTVAGGELVNLPGISYGLAGRRWLLRLMRWAMQQADVVTAGASG
                     LLDAVRNVTGCDADHLAFAPLGVDTAMFHPANRRPEMPAVLVNVGALQAVKGHADLIR
                     AFRFVVDREPNVRLRIVGAGELRAELEALSAELNLNDHVHFVGEVRHDQLPDVYRRAT
                     MFVQASYHEAQGMAVLEAAACGLPIVGTCVGALADLAPDAAVATPPGDPIRLGQNILT
                     MLGDPARLRALGHAAQDRAQKIYSLEASVARFESLYGLRL"
     gene            complement(1241..2851)
                     /locus_tag="AC162G_01345"
     CDS             complement(1241..2851)
                     /locus_tag="AC162G_01345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR5074491.1"
                     /translation="MYENQSAGKAHFFVVAGCLVLYLLVALRHLEILPPVYEDEPWLA
                     STGLKLATRGIFGSDMFAGFYGMESHCYACMPLHPSLLAVTYRLVGFGLFQTRLEPVM
                     MGLLALALTYALGRRLFGPGVGLLAVGLMLFTRFTAAAPSRLTGILLLDVARIARYDI
                     VVSVCGLASLHAYVSARKRADARLYALAGLMAGLSGLAHVYGAFWIVALAILAVWDRA
                     ARRAFVALAIGFVAPWLVYLVYVLQNPADWAGQVRGYAPRFDLLNPAWYWDNLRNEYQ
                     RYAPGIDSIRQVATRPGLALITVVWPAALLALFRRAIRLSDGAARTLAVPALVMPALF
                     ALLLHIKMSNYLIAVLPLGALVVAWGLVSLWRWAGRTWQPFASRWMQVAVAGLTCAVF
                     AEGIARIGMLESLAATTTPYPEFIARVRAFVPHDARVLGLHNYWLGLHGLDYRSWLVP
                     VLQADARYSSAPLPVEAVLDRIAPDVILLDPRMRAYLDDPGAGDPVGGIRDWMERQHF
                     VRIGVVNDGTYGVMEILARPGVSSPSSP"
     gene            complement(2806..>3555)
                     /locus_tag="AC162G_01350"
     CDS             complement(2806..>3555)
                     /locus_tag="AC162G_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074492.1"
                     /translation="GAWRYALPIGLFYLQPLVVPSYVFPLFIPLWLAGLAALSRLDAP
                     VRALLIGWPLVVYMFLAGIAWQNPRFALAFFPPLAVWVGVGFDRVWESRPAWRKGLVG
                     LAAIALVGTLAWSGRVVANFAHAKAADLARVERVSSRLPGGSRVITFGVSLSLDHYTD
                     LEVIDLYSESPESLSDRLSDCVSAFVYVDVENLERQWRGLSPEVNYRWLREEAGLEEV
                     DRFQDYTLYSVTRNGASCTRISQPARLTSSS"
BASE COUNT          776 a         1190 c         1072 g          517 t
ORIGIN      
        1 cctaggcagg taacgttatt gtctatcgcg tctcctatta acaggaaacg ttacgcacag
       61 actgtcgcca gggattggga gtagacctgg caggtcttcg cagacaaggc ctgccaggtc
      121 tatagcctca ggccgtacag cgactcgaag cgggcgaccg acgcctccag gctatagatc
      181 ttctgggcgc gatcctgcgc cgcgtggcct agggcccgca ggcgcgccgg atcgcccagc
      241 atcgtcaaga tattctggcc caatcgaatc gggtcgccgg gcggcgtcgc cacggcggca
      301 tcgggcgcca gatcggccag cgcgccaacg catgttccga cgatcggcag gccgcaggca
      361 gccgcctcca gcacggccat accctgcgct tcgtgatacg acgcctgaac gaacatcgtc
      421 gccctgcgat acacgtcagg caactgatcg tgccgcacct cgccgacaaa gtgcacatga
      481 tcgttcaggt tcagttctgc gctcaaggct tccaattccg cgcgcaactc ccctgccccg
      541 acgattctca acctcacgtt cggttcacgg tcgacgacga agcgaaaagc cctgatcaga
      601 tcggcgtgtc ccttgacggc ctgcaacgca cccacgttga cgaggacggc aggcatctcc
      661 gggcggcggt tagcggggtg aaacatcgcc gtgtcgacgc ccagaggcgc aaaggcgaga
      721 tgatctgcgt cgcagccggt gacgttgcgc acagcgtcca gcaatccgct cgctccagcg
      781 gtgacgacat ccgcctgctg cattgcccag cgcataaggc gcagcagcca gcgccgtccg
      841 gccagcccgt atgagatgcc gggcagattc accagttcgc cgccggcgac ggtcacgatc
      901 acacgcacgc caagccgccg cttcagccag gcggcgatga ggcccggctc gtacacccaa
      961 aacgcatgca tgacatcgaa tcggccgcga cgctgctcaa gcactatctc gttcgccgca
     1021 gctttcagaa cgccgggcag ccgccagcct ctgcggttcg ccccgccgat cgcatgcacg
     1081 cgcgctccga aaacggaata gtgatcgcgc cggtaagggt aatgcagcgc gaacacgtgc
     1141 acttcgtgcc gcgccgccag ggcacgcgcg agatggagca gcgccgggat gcaccagtcg
     1201 ttctcgtcgc tgctgaagcc ggggacgatc agtccgatcc tcatggcgaa ctcggcgacg
     1261 acacacctgg ccgagccaga atctccatca cgccgtacgt gccatcgttg acgacgccga
     1321 tccgcacgaa atgttggcgc tccatccagt cgcggatacc gcccaccgga tcgcccgcac
     1381 ccggatcgtc caggtaggcg cgcatgcgcg gatcgagcag gatcacgtct ggtgcaatgc
     1441 gatcgagaac ggcttcgacg ggcaacggcg cggatgagta tctcgcgtcc gcttgcagca
     1501 ccggcaccag ccaggatcga tagtcgagac cgtgcagccc cagccagtag ttgtgaaggc
     1561 ccagcacgcg ggcgtcgtgc ggaacgaagg cacgcacgcg cgcaatgaat tcgggatacg
     1621 gcgtcgtggt cgccgcaaga ctctccagca tgccgatgcg cgcaatccct tcggcgaaca
     1681 cagcgcacgt gagaccggcc acggccacct gcatccagcg cgaggcgaac ggctgccatg
     1741 tgcgtcccgc ccatcgccaa agcgatacca gtccccatgc gaccaccagc gcgccgagcg
     1801 gcagcactgc gatcaggtaa ttgctcattt tgatgtgaag gagcagggcg aataacgcgg
     1861 gcatgaccaa cgccgggact gcgagtgtcc gcgctgcgcc gtcggacagc cggatcgccc
     1921 gcctgaaaag ggcgagcagt gccgcaggcc acacaaccgt tatcagcgcc aagcccggtc
     1981 gcgtcgcaac ctgccgaatc gagtcaatgc ccggcgcgta gcgctggtat tcgtttcgca
     2041 ggttgtccca ataccacgcc gggttgagca ggtcgaagcg aggcgcgtaa ccgcgcacct
     2101 ggcccgccca gtccgcagga ttctggagca cgtagacgag gtacaccagc cagggtgcaa
     2161 caaaacctat tgccagtgca acgaatgcgc gccgcgcggc tcggtcccac accgccaaaa
     2221 tcgcgagcgc gacgatccag aaagcgccat acacatgagc cagccccgag agtccggcca
     2281 tcagtccggc aagcgcgtac agccgcgcgt cggctcgttt gcgcgccgac acgtacgcgt
     2341 gcagcgacgc caatccacac acagacacga caatgtcgta gcgggcgatg cgtgcaacgt
     2401 caaggagcaa aatgcctgtc aggcggctcg gcgcggcggc tgtgaatcgg gtgaaaagca
     2461 tcaagccgac tgccagcagg ccaacgcctg ggccgaatag ccgccgcccc aaagcatacg
     2521 tcagggcgag cgcgaggagt cccatcataa ccggttcgag ccgagtctga aagaggccaa
     2581 acccaaccag gcgataggtg acggccagga gtgaggggtg aagcggcatg cacgcatagc
     2641 aatgcgactc cattccgtag aagcccgcga acatatccga tccgaaaatg ccacgcgtcg
     2701 ccagtttcaa gccggtggag gccagccacg gctcgtcttc atagactgga ggcagaatct
     2761 ccagatggcg cagcgcgaca agcaggtaaa gaaccagaca gcccgctacg acgaagaagt
     2821 gagccttgcc ggctgactga ttctcgtaca tgatgcgcca ttcctcgtca cgctgtacaa
     2881 tgtgtaatct tgaaagcggt ctacctcctc aagcccggcc tcctcgcgca gccaccgata
     2941 gttcacttca ggcgacagcc cacgccactg ccgttcgagg ttctccacat cgacataaac
     3001 aaacgccgag acacagtcgc tcaggcgatc gcttagcgat tccggcgatt cggaatatag
     3061 atcgatgacc tccaagtccg tatagtgatc gagcgacagg ctgacgccga aagtgatcac
     3121 acgtgatccg cccggcagcc gagacgatac acgctcgacg cgagccaggt cggcagcctt
     3181 ggcgtgggcg aaattggcga ccacgcgccc ggaccaggcg agcgtcccga ccagcgcgat
     3241 ggccgccagg ccgaccagcc ccttccgcca tgccgggcgg ctctcccaga cccgatcgaa
     3301 cccgacgccg acccagaccg ccagcggtgg aaagaatgca agcgcaaaac gaggattctg
     3361 ccaggcgatc ccagccagaa acatgtacac gaccagcggc cagccaatca gcagagcgcg
     3421 gacgggagcg tctaatcggc tgagcgcagc cagaccggcc agccacaatg gtatgaacag
     3481 cggaaatacg tagctcggaa cgaccagcgg ttgtaggtag aacaggccga tcggcagtgc
     3541 atagcgccac gcgcc
//
LOCUS       DBNHXB010000033         4842 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3195081, whole genome shotgun sequence.
ACCESSION   DBNHXB010000033 DBNHXB010000000
VERSION     DBNHXB010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3195081"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="AC162G_01355"
     CDS             <1..875
                     /locus_tag="AC162G_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HIR5074493.1"
                     /translation="WIERYAAWLKSYPDQGGPASVEAFLTHLAVAEHVSASTQNQARS
                     ALLFLYRDVLRQDPGAIHAVRAKRGVRVPIALTHAEALRLINALRGDYQLLAQLMYGS
                     GLRVMEALRLRVKDVNFARLQITVRDGKGDKDRFTILSPSLVEPLRAKLAERRALHRR
                     DLAAGLGEAYLPDALARRYPNAPREFSWQFIFASPRLTTDPRDGKPRRHHILPTNVQR
                     QIKRAAHTLGLDKRAPIGPHTLRHSFATRLVEKKAPIHEVKELMGHASIETTMIYLRA
                     AEHSGHTVVSPLDT"
     gene            881..2143
                     /locus_tag="AC162G_01360"
     CDS             881..2143
                     /locus_tag="AC162G_01360"
                     /inference="COORDINATES: protein motif:HMM:NF016310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HIR5074494.1"
                     /translation="MLKLTSHDEALLAGKHGPAAQLAMSIVTRMAEVYGAPELIDITG
                     AHIDSAIYIGEAGLEFAERLAGLGAKVAVPTTLNVSGLDEHHWQEWAVPADWAPKAHR
                     QMLAYQSMGCIPTWTCAPYQTEMRPSFGQQIAWGESNAIVFANSVLGARTERYPDLLD
                     ICCAITGRVPAVGLHLTENRAGEILLRLAGVPITLQNDDSFYAVLGHLTGKIAQDRIP
                     VVDGLAAQPTEDQLKALGAAASSSGAVGLFHLVGVTPEAPTLEAAFQDRRPQQAIDVT
                     LDDLRQARRELTTAGDEQLHMVVLGSPHFSLAEFKRLAPLLVGKRAHPDVKFLVTSSR
                     AMTLLARRYGYLQALEDFGGRVTVDTCILASPMLPPEVRLLMTNSAKYAYYAPGLLGA
                     RVTFGSLEDCVRSAVAGRVVRDESLWET"
     gene            2140..2571
                     /locus_tag="AC162G_01365"
     CDS             2140..2571
                     /locus_tag="AC162G_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014091.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X swivel domain-containing protein"
                     /protein_id="HIR5074495.1"
                     /translation="MIRGRVVIKGSAEGEALVSHEPLSFWGGYDYRTGEIIDRRHPLS
                     GHIAAGRVLAVPFARGSSTTTAVLLEAVRAGTAPAAIVTTGVDSFFALASIVADEMYA
                     KPIPLIALEPDDFARLRTGTRVRIDEAGTIVAEEAFTANET"
     gene            2712..3296
                     /locus_tag="AC162G_01370"
     CDS             2712..3296
                     /locus_tag="AC162G_01370"
                     /inference="COORDINATES: protein motif:HMM:NF047646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase"
                     /protein_id="HIR5074496.1"
                     /translation="MPNFRRYYIPNAIVFITVVTGGRFPFFATEDNVVLLFETMRLVQ
                     EIHPFRLLAYVVLPDHLHWLMRVDEKSGNFSTVMHSIKRNTTLNYKKARGITGSLNLW
                     QERFWDHIVRDEHDFDNHFDYIHWNPVKHGYVQRPEDWPHSTMLGPLRIRVTIVVDLR
                     CGNVHVPAPSLADQLPRLRPARTRAARRPDSHPR"
     gene            3205..4419
                     /gene="recF"
                     /locus_tag="AC162G_01375"
     CDS             3205..4419
                     /gene="recF"
                     /locus_tag="AC162G_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014516.7"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR5074497.1"
                     /translation="MYLRHLSLTNFRAYARLELELPAGPILIHGDNAQGKTSLLEAVY
                     YLATGKSPHTSSDRQLIHWLAAEESGAPYARLVGDVQRADRAIHLDVTLLLEPASTID
                     GYRFRKQIKLNGAHARLADLAGQVSAVLFLPQDVDTVSGSPGERRRYLDNAVGQIDPD
                     YARAIDRYADVLAQRNALLKQLQESEGDPDQLAYWDDRLVADGAAITLKRQIALDELS
                     RLADRIHRDLTGGRVDLRLRYQPAFDAARSSIPERQIAMRLDVPQVAMTDARRLESAF
                     QAQLQERRRDEINAGVTLVGPHRDEFRFISDQIDLGTYGSRGQQRTAILALKLAEVEW
                     VHQKTGDWPILLLDEVMAELDAQRRAYLLARLGEANQSLVTTADLAMFDAPYRTRAKL
                     LHVTAGRIEEVY"
BASE COUNT          870 a         1690 c         1447 g          835 t
ORIGIN      
        1 gctggattga gcgctacgcg gcctggctca agtcctatcc tgatcaaggc ggccccgcca
       61 gtgtcgaggc gttcctcacc catctggccg tcgcagaaca cgtcagcgcc tccacccaga
      121 atcaagcccg ctcggcgctg ctcttccttt accgcgacgt gctcagacaa gatcccggcg
      181 ccatccacgc cgtgcgcgcc aaacgcggcg tccgcgtccc tatcgccctc acccacgccg
      241 aagccctgcg cctgatcaac gccctgcgcg gcgactatca actgctcgcg caactgatgt
      301 acggctccgg cctgcgtgtc atggaagccc ttcgcctgcg cgtgaaagat gtgaactttg
      361 cacgcctgca aatcacggtg cgcgacggca aaggcgacaa agaccgcttc accatcctgt
      421 cgccttcgct ggtcgaaccg ctgcgcgcga agctcgccga gcgccgcgcc ctccacaggc
      481 gcgatctggc cgccggcctg ggcgaagcct acctgcctga tgctctcgcc cgtagatacc
      541 ccaacgcccc gcgcgagttc agctggcagt tcatcttcgc ctcgccgcgc ctcaccacgg
      601 atccacgcga tggcaaaccc aggcggcatc acatcctgcc caccaatgtc cagcgccaga
      661 tcaagcgcgc cgcgcacacg ctcggcctcg acaagcgcgc ccccatcggc ccgcacacgt
      721 tgcgccactc ctttgccacc cgcctcgtcg aaaagaaagc tcccatccac gaagtcaaag
      781 aactgatggg acatgcctcg atcgaaacga cgatgatcta cctgcgcgcc gccgagcaca
      841 gcggtcacac cgtcgtcagc ccgctcgaca cctgatcccc atgctgaaac tgacctccca
      901 cgacgaagcc ctgctcgcag gcaaacacgg cccggccgcg caactggcaa tgtcgatcgt
      961 cacccgtatg gccgaagtct acggcgctcc ggaattgata gatatcaccg gcgcgcacat
     1021 cgacagcgcg atctacatcg gcgaagccgg gcttgaattc gccgagcggc tggcgggcct
     1081 cggcgcgaag gtcgccgtgc ccacgacgct gaacgtcagc ggcctcgacg agcatcactg
     1141 gcaggagtgg gccgtgcccg ccgactgggc gcccaaggcg catcggcaaa tgctggctta
     1201 ccagagcatg ggctgcatcc ctacctggac gtgcgcgccg tatcagaccg agatgcgccc
     1261 ctcgttcggc cagcagattg cgtggggcga gtcgaacgcg attgtgttcg ccaactccgt
     1321 cctcggcgcg cgcaccgagc gctatcccga cctgctcgac atttgctgcg cgatcacggg
     1381 acgtgtcccg gctgtcgggc tgcacctgac cgagaaccgc gcgggcgaga ttctcctgcg
     1441 cctggccggc gtcccgatca cgctgcagaa cgacgacagc ttctatgccg tgctcggcca
     1501 tctcaccggg aagatcgcgc aggatcgcat tccggtcgtc gacggcctgg cggcgcagcc
     1561 aaccgaggat cagctcaagg cgctcggcgc ggcggcgtcc tcttccggcg cagtcgggct
     1621 gttccacctg gtcggcgtga cgcccgaagc accgacgctg gaggcggcct ttcaagatcg
     1681 caggcctcag caggcgatcg acgtgacgct ggacgatctg cgccaggccc gtcgcgagct
     1741 gacgactgcc ggggacgagc agttgcacat ggtcgtgctg ggcagcccgc acttttcgct
     1801 cgccgagttc aagcggctcg cgccactgct ggtcgggaag cgcgcccacc cggatgtgaa
     1861 attcctggtc acctcgagcc gcgcgatgac gctcctcgcc aggcgatacg gctatcttca
     1921 ggcactggag gatttcggcg ggcgcgtgac cgtggacacc tgcatcctgg cctcgccgat
     1981 gctgccgccg gaggtcaggc tgctgatgac aaactctgcg aagtacgcct attacgctcc
     2041 cggcctgctc ggtgcgcgcg tcaccttcgg cagcctggaa gactgcgttc gctccgccgt
     2101 ggccggtcga gtcgtgagag atgaatcgtt gtgggaaacg tgatacgcgg tcgagtcgtc
     2161 atcaagggca gcgccgaggg tgaggcgctc gtcagccacg agccgctgtc cttttggggc
     2221 ggctacgatt atcgaaccgg cgaaatcatc gaccggcggc atccgctgtc cggccacatc
     2281 gcggcggggc gggtactggc cgtgcccttc gcacgcgggt catcgacgac gacagccgtg
     2341 ctgctggaag ccgtgcgagc cggaaccgcg cccgcggcta tcgtgacgac cggcgtcgac
     2401 tcgtttttcg cgctggcgtc gatcgtcgcc gacgaaatgt acgcgaagcc catccccctg
     2461 atcgccctgg aacccgatga cttcgcaaga ctgcgaacag gcacgcgggt gaggattgac
     2521 gaggctggca ccatcgtggc ggaagaagcc ttcaccgcga atgaaacgta gggcggcttt
     2581 ccatagccgc cgcaggtcgg gcaggtatgg aaacttccct acggtgattg agacgtaggg
     2641 cggctttcta tagccgcctc agccgggcag gtatggaaac ctgccctacg tgaatgcgac
     2701 gggggttgcg catgccgaac tttcgccgat actacattcc gaacgccatt gtgttcatca
     2761 cggtcgtgac cggcggtcga ttcccattct tcgcgacgga agacaacgtc gttctgctgt
     2821 tcgagactat gcgcctcgtg caggagattc atccatttcg tctcctcgcc tatgtcgtct
     2881 tgccggatca cctccactgg ctgatgcgtg tcgatgagaa gagcggcaat ttctctactg
     2941 tgatgcatag cataaagcgc aataccacgc tcaactacaa gaaggcgcgt ggcatcaccg
     3001 gctcactcaa cttgtggcag gagcgcttct gggatcacat cgttcgcgac gagcacgatt
     3061 tcgacaacca cttcgactat atacactgga atccggtcaa gcacggttac gtccagaggc
     3121 cggaggattg gccgcattct acgatgctcg gcccgctgcg cattcgtgtt acaattgtcg
     3181 tcgatctccg ctgcggcaac gttcatgtac ctgcgccatc tctcgctgac caacttccgc
     3241 gcctacgccc ggctcgaact cgagctgccc gccggcccga ttctcatcca cggtgacaat
     3301 gcgcagggca agaccagcct gctcgaagcc gtttactacc tcgccaccgg caagtcgccg
     3361 cacacgagca gcgaccggca gttgatccac tggctggccg ccgaagagag cggcgcgccc
     3421 tacgcgcgcc tcgtcggcga cgtgcagcgg gccgatcgcg ccatccacct cgacgtgacg
     3481 ctcctgctcg agccggcgag cacgatcgac ggctaccgtt tccgcaagca gatcaagctc
     3541 aacggcgctc acgcgcgcct agccgacctg gccggccagg tcagcgcggt gctcttcctg
     3601 ccgcaggacg tggataccgt ctccggctcg ccgggcgagc gccgccgtta cctcgataac
     3661 gccgtcggcc agatcgatcc cgactacgca cgcgcgatcg accgctacgc cgacgtgctg
     3721 gcgcagcgca acgcgctgct caagcaattg caggagagcg aaggcgatcc tgatcagctc
     3781 gcctactggg acgaccggct cgtggccgac ggcgcggcga tcacgctcaa gcggcagatc
     3841 gcgctcgacg agttgagccg gctggccgat cgtattcatc gcgatctgac cggcggccgg
     3901 gtcgacctgc ggctgcgcta tcagcccgcg ttcgacgcgg cccgttcctc catccctgag
     3961 cgccagatcg ccatgcgcct cgacgtgcca caggtcgcca tgacggacgc gcggcgtctc
     4021 gaatcggcgt tccaggcgca attgcaggag cggcgccgcg acgagatcaa cgccggtgtg
     4081 acgctcgtcg gcccgcaccg cgacgagttt cgcttcatct ccgatcaaat cgacctcggc
     4141 acgtacggat cgcgcggcca gcagcggacg gcgatccttg cgctcaagct cgccgaggtc
     4201 gagtgggttc accagaaaac cggcgactgg ccgatcctac tgctcgacga ggtgatggcc
     4261 gagctggacg cgcagcgccg ggcctacctg ctggcgcgat tgggcgaggc caaccaatct
     4321 ctggtgacca ctgccgacct cgccatgttc gacgccccct atcgcacccg ggcgaagctg
     4381 ctgcacgtta ccgcaggtcg catcgaagag gtgtattgac attctcatcg atctggttat
     4441 actcatccct atccgacccg ctgccgtgcc cgcaactgag aagcctattg cgccgtcgaa
     4501 cccggcctgc cgcgttcgcg ccagagggag gcgcgctacc gccagcgatc agtctaggtt
     4561 cgagacccgt gaggagattc tcaatgtaca ccaagcccga gatcaccaag cacgaagacc
     4621 tgtcccaggt cacctttagc agccactaat cgcgcggtgg cgttggccac ctagcacgac
     4681 tgcagctaac cgaaggaccc ggtcacatga ccggtcagcc gttgcactcg tagcgcgcct
     4741 ccctctggtc aacagcgccc atggcttccg gcctgtctgc gacgcagctc attctcgacg
     4801 ccttgcgtcc tcaccagact cccatgacct gggagtctga tg
//
LOCUS       DBNHXB010000034         9451 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_678790, whole genome shotgun sequence.
ACCESSION   DBNHXB010000034 DBNHXB010000000
VERSION     DBNHXB010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9451)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9451)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9451
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_678790"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="AC162G_01380"
     CDS             complement(<1..523)
                     /locus_tag="AC162G_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074498.1"
                     /translation="MKDHLRIGIVVTGALLMLVGLVVVACTPAGPTATTAPTTPAGPA
                     ATTAPTTPPEPELAGDAVRGGQLYDTWWAVAPMEEEEHKEGEEHEAAGPTTDQPLWKT
                     QTTNTRTGEETWRCKECHGWDYRGVDGAYGSGSHKTGFVGIFNAQSKPAAELLAILKG
                     STNPDHDFSKDIDE"
     gene            complement(627..2570)
                     /locus_tag="AC162G_01385"
     CDS             complement(627..2570)
                     /locus_tag="AC162G_01385"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5074499.1"
                     /translation="MGEAAYRAFAPDLTFYQQTVACQFACPVRTDGRAYVTAIARGEY
                     ERAYLIARETNPFASTCGWVCGAPCEAACRRGKIDAPIAIRALKRFVNDRYGVYLGES
                     GRTRQPPAWPGYVGPTDAFDLGNTTFPTSRSGLAAARRRSPKTGKHVAVVGAGPAGLS
                     CAHDLTLLGHEVTIFDAASITGGMLRLGIPEYRLPRELIDLEIQSILALGPTLQLNQA
                     LGRDFTLADVRRDYDATFIAIGTYKSRKLNVEGEDLDGVLRAVDFLINVNLGGYNLDL
                     GRRVLVIGGGNVAMDVARTAARLGHPAQSGGDLEVALDVARAARRLGATQEVRCLVVE
                     DRHEMLADPFEIAEAEEEGILIHNHAAPKRIVGGNSRAVGVETLDVARAFDERGRFNP
                     QLIPDTERVWECDSVIVSIGQTGALDWIRPADGLEVTPRGTLAVDRETLMTTAPGVFA
                     GGDIAFGPRLIINAVADGQRAARGIHAYLQHVQPRLVRKAFFTPISKRQYPGSGPLRD
                     YLRWPRRQPPSLPVERRIGVAKVEIGFDEAAAREQGGRCLICTLNPIFDGELCILCNG
                     CVDVCPTDCLKLVPASALQGDEHVAAVIAHRSSEWPAASAMLLDPTRCIRCGLCAARC
                     PTEAVQMESFRFTEEVLFEPADA"
     gene            complement(2643..3323)
                     /locus_tag="AC162G_01390"
     CDS             complement(2643..3323)
                     /locus_tag="AC162G_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5074500.1"
                     /translation="MAQQTILLIDDDTTLLELLAGHLQAAGYRPLTALDGRNGLRLAA
                     EAQPHLVVLDVMIPGTDGWTVCQQLREKSRVPIIMLTAKGEEFDKLRGFRLGVDDYVT
                     KPFSFAELVARVGAVLARASQAPMSAHRLTSDDLAIDFDRRRVTVADRAIELTPTEYR
                     LLETLARHANRALPTEALLAEVWGPEYAGETEHVKHYIWALRKKIEADPGDPKHILTE
                     RGFGYRFE"
     gene            complement(3326..4747)
                     /locus_tag="AC162G_01395"
     CDS             complement(3326..4747)
                     /locus_tag="AC162G_01395"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR5074501.1"
                     /translation="MFVTGPALRQRIAKLRWVLPLGFSALATLYQLLWARWVHDTYGD
                     PAHFAIEILFYSTAGPLLAFWMLTLIGRWLDEKERAERQAQASEQRLASITSASADAI
                     LSLDDQGRIASWNRGAELIFGYSAHEIRDRPFTDLYREGETAEVEFRWLVEAVREAGF
                     VRGHETTCRDATGRTIAVELTATRLPGESGRRPAMSIILRDITDRKRREEEIRRLNAS
                     LNRQVAERTRELAEKVEELARANEDLQKLDRMRSEFVSLVSHQLRAPLTNMHGAVEHI
                     EANCSVMNATCSRMLSVLNQQVRRLDRLVRDVLSAARIEAGDVVLQPEPISVLPVVRQ
                     VTEQIRARTTRRPFRLPTKPGLPLAFADRDRTAEVIANLLDNADKYSPPGKDVVIDVQ
                     ADEIEVIVSVRDFGSGLPPSDLDRVFQKFYRTDGSDAQAAYGYGLGLYICRQLVEAQG
                     GRIWAENHPDGGAIFSFALPVAS"
     gene            complement(4782..5300)
                     /locus_tag="AC162G_01400"
     CDS             complement(4782..5300)
                     /locus_tag="AC162G_01400"
                     /inference="COORDINATES: protein motif:HMM:NF019350.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HIR5074502.1"
                     /translation="MSDFSKRLTLGRMQNPRRERRLSHVAFRRRFRLLQKLVPAALVL
                     LVVIYEIGPARWIHHVLGEDHHFVAEILVYGTVGPALAFLLLDFVGRWLDERDTSEFQ
                     AQVLADARESERIGRELSDEALQTLFAASALLASLKSTLPEVRTETAGALHETEEALD
                     RAIQKLRDHLQT"
     gene            complement(5366..5521)
                     /locus_tag="AC162G_01405"
     CDS             complement(5366..5521)
                     /locus_tag="AC162G_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074503.1"
                     /translation="MVINPGERTWVSMQFMMHGNMGGMHDFRLHLPTNDPANPQKEVV
                     VLSNWVQ"
     gene            complement(5546..5896)
                     /locus_tag="AC162G_01410"
     CDS             complement(5546..5896)
                     /locus_tag="AC162G_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074504.1"
                     /translation="MSQKKKRKPGASRHKTSQSKAPLLIIAGGVALVAIAALLIFGGG
                     APASPPATVEVSGTPAAIEVSGAPALKVDKEKVDLGTVRLGEWVTVNFELANAGDQTL
                     RFTEQPYVTVVEGC"
     gene            complement(5893..6897)
                     /locus_tag="AC162G_01415"
     CDS             complement(5893..6897)
                     /locus_tag="AC162G_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5074505.1"
                     /translation="MSAERIARTIVIVLAVGLPVAAVIAGKAESVAGQTIELHGTMSE
                     TGGWTPSDITAKAGEPIRLRLTSDDVIHGFAIGQSDLPALDVKPGEVIETTLTFDEPG
                     KVTFYCTRWCGPNHWRMRGTIEVISDQSVSDQWAVEQPLYVRLGIDIDAPHPTDVLPD
                     RAPSAQRGAALGIAIPVNYLTAEFYRTHSPADAWKELRDEPFTQGLTEGQVWDLVADV
                     WRSNTTAQALETGKKLFAQNCAACHGENGAGDGVMAASLTGETGAGDMQRHDTTAPVD
                     FTDASSMLGASPALLHGKIVRGGMGTGMPYWGPIFTEDQLWSVISYLWTFQFDLEGNG
                     "
     gene            complement(6894..8267)
                     /locus_tag="AC162G_01420"
     CDS             complement(6894..8267)
                     /locus_tag="AC162G_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024211.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR5074506.1"
                     /translation="MVQYPRFDLAELPLVKPALRSRWPQFLVRVIALAGFVFAIVAGL
                     AGTPVGSRNFGIVFVWIAWWAVLMLIAVPLFGRGWCSICPIPLPGEWLQNGAVLGPQG
                     RGFSLGRRWPRRLRSMWIQNGAFVAVALFSLVVLTQPGVTALVLALFLVLAIVTSLIF
                     ERRAFCRYLCPVGGFIGLYSQLAPIEVRAKDAALCASHTEKTCYTGSAGGYGCPWQVF
                     PGGLAKNTYCGACVECLRTCPHDNIALNLRPFGADLLKPSGRKLDEAFKAFIMLGSAV
                     VYSAVMLGQWGELKAAAYSIGSVEWLVYAVSFLAGVLVALPGLFLFAVHLGRRLSGSK
                     MKSKKAFTAFAYALVPLGLAAWIAFSISFVLANLSYVWPALSDPMGEGWNLLGTAGIA
                     WAPYLTQVVPVLQATALLGGLAWASMTARRIAGEQLPKRTATLQALPIIAFCLLVTIG
                     LLGLLIG"
     gene            complement(8415..8867)
                     /locus_tag="AC162G_01425"
     CDS             complement(8415..8867)
                     /locus_tag="AC162G_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5074507.1"
                     /translation="MTRTKVSRRWRWPIVILAGLAILLVPIPALSAAPGERYFRVEAS
                     SFEYTPAEIAVNPGDRVTIELASTDVVHGLYLDGYDLNTTADPGQPARLTFTADKAGA
                     FRFRCSVTCGALHPFMIGKLTVGENDLLWRAIGLAVLATFAGVWLFRK"
     gene            complement(8892..9347)
                     /locus_tag="AC162G_01430"
     CDS             complement(8892..9347)
                     /locus_tag="AC162G_01430"
                     /inference="COORDINATES: protein motif:HMM:NF025170.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HIR5074508.1"
                     /translation="MFYQRRFFLLLVTGLLALGGLATGCDASASGDTAAHPFQMADLA
                     PMPVEVQTAPVAVQQAYQFAVANPDVLKQIPCYCGCGDMGHTSNYACYVAGTNAGGTL
                     KYDPHALGCSICVDITQDTMRLLRQGKSVSEIRAIVDETYSRYGPSNMP"
BASE COUNT         1643 a         3054 c         3090 g         1664 t
ORIGIN      
        1 gttcgtcgat gtccttcgag aagtcgtggt cggggttagt gctgcccttg agaatggcga
       61 gcagctcggc ggcgggcttg ctctgggcat tgaagatgcc gacaaagccg gtcttgtgcg
      121 agccgctgcc gtacgcgccg tcaacgcccc tgtaatccca gccgtgacat tctttgcagc
      181 gccaagtctc ttcaccggtg cgcgtgttgg tggtctgcgt cttccacaag ggttgatcgg
      241 tagttggccc ggcagcctcg tgttcctccc cttctttgtg ctcttcctct tccatggggg
      301 ctaccgccca ccacgtgtcg tagagctggc cgcctcgcac cgcgtcgcct gcaagctccg
      361 gttcaggcgg agtggttggc gcggtggtcg ccgcaggccc tgccggcgtg gtcggtgcgg
      421 tggtcgccgt aggccctgcc ggcgtgcagg ccaccacgac caggccgacc agcatcagca
      481 gcgcgcccgt cacaacgatt ccgatcctca ggtggtcttt catatggcat ctcctcacgg
      541 tgcatgaaga cggcggatag agacagtggg tgcgcgtttg tttttcattg ccctcctctc
      601 agataggagg cagatcgcat gtttgcctat gcgtctgccg gctcgaacaa cacctcttcc
      661 gtgaagcgga acgattccat ttggacggcc tcggtcgggc agcgcgcggc gcacaggcca
      721 cagcggatgc agcgcgtcgg gtcgagcagc atggccgaag ccgccggcca ttcagatgat
      781 cggtgcgcga tgacggcggc cacgtgctcg tcgccctgca gcgccgaggc gggcacgagc
      841 ttcaggcagt cggtcgggca cacgtcgacg cagccgttgc acagaatgca cagctcgccg
      901 tcgaagatcg ggttgagcgt gcagatcagg cagcgcccgc cctgctcgcg cgccgccgct
      961 tcgtcgaacc cgatctcgac ctttgccacg ccgatgcgcc gctcgaccgg cagcgacggt
     1021 ggttggcggc gcggccagcg caggtaatcg cgcagcgggc cgctccccgg atactggcgc
     1081 ttcgagatgg gcgtgaagaa ggctttgcgc acgaggcgcg gctggacgtg ctgcagatag
     1141 gcgtggatgc cgcgcgcggc gcgctgaccg tcggcgacgg cgttgatgat caggcgcggg
     1201 ccgaaggcga tgtcgccgcc ggcaaacacg ccgggcgcgg tcgtcatcaa ggtctctcgg
     1261 tccaccgcca gcgtgccgcg cggtgtgacc tccagcccgt ctgcaggccg tatccagtcg
     1321 agcgcgccgg tctgaccgat cgagacgatc acgctgtcgc actcccaaac cctttcggtg
     1381 tccgggatca gttgtggatt aaagcggcct cgctcgtcga aggcgcgggc cacgtccagc
     1441 gtttcgacgc cgacggcgcg gctgttgccg ccgacgatgc gcttaggcgc agcgtgattg
     1501 tggatgagga tgccctcttc ttcggcctcg gcaatttcaa acggatcagc cagcatctcg
     1561 tgccggtctt caacgactaa acaacgcact tcctgcgtcg cgccgaggcg gcgggcggcg
     1621 cgcgccacgt cgagcgcgac ttcgaggtca ccgcccgact gtgccggatg gccgagtcgt
     1681 gcggcggtgc gcgccacgtc catcgccacg ttgccgccgc cgatcaccag cacgcgccgg
     1741 cccaggtcga ggttgtagcc gccgaggttg acgttgatca gaaagtcgac ggcgcgcagc
     1801 acgccgtcca ggtcctcacc ttcgacattg agtttgcggc tcttgtacgt gccgatggcg
     1861 atgaaggtcg catcatagtc gcggcgcaca tcggcgagcg taaaatcgcg cccgagcgcc
     1921 tgattgagtt ggagcgtcgg gccgagcgcg aggatcgatt ggatttcgag gtcgatcagt
     1981 tcacgcggca ggcgatattc ggggatgccc aggcgaagca tgccgcccgt gatggaggct
     2041 gcatcgaaga tcgtcacctc gtgtccgagc agcgtcaggt cgtgcgcgca cgacaggccc
     2101 gccggacccg caccgacgac ggcgacgtgc ttgccggtct tggggctgcg ccggcgcgca
     2161 gcggcgagac cgcttcgact ggtggggaag gtcgtattgc cgaggtcgaa ggcatccgtc
     2221 ggcccgacat agcccggcca ggccggaggc tgccgggtcc ggccgctctc gccgaggtac
     2281 accccgtagc gatcgttgac gaagcgcttc agcgcgcgaa tcgcgatggg cgcgtcgatc
     2341 ttgccgcgcc ggcaggcggc ctcgcacggc gcgccgcaca cccagccgca ggtcgaagcg
     2401 aagggattgg tctcccgcgc gatcaggtaa gcccgctcgt attcgccgcg cgcgatggcc
     2461 gtgacgtagg cacgcccgtc ggtgcgtacc gggcaggcga actggcaggc gaccgtttgc
     2521 tggtagaagg taaggtcggg cgcgaacgcg cggtaggcag cctcgcccat ggggcctcct
     2581 gccgcgcagt gtatcaggcg aaggtgtcga ttaggtgtac gtgaggtgtg ggttgagtga
     2641 ggtcactcga atcgatagcc gaagccgcgt tcggtgagga tatgtttcgg gtcgccggga
     2701 tcggcttcga tcttcttgcg cagcgcccag atgtaatgct tgacatgttc ggtctcaccg
     2761 gcgtattccg gcccccacac ttcggccagc agggcctcag tcgggagcgc gcggttcgcg
     2821 tggcgggcga gcgtttcgag caggcggtat tcggtcgggg tgagttcgat ggcgcggtcg
     2881 gccaccgtga ctcgccgccg gtcgaagtcg attgccagat cgtcgctggt cagacggtgc
     2941 gccgacatcg gcgcctgact ggcgcgcgcc aggactgctc cgacgcgcgc tacgagttcc
     3001 gcgaacgaga atggcttcgt cacgtagtcg tccacgccga ggcgaaagcc gcgcagcttg
     3061 tcgaactctt cgcctttggc cgtgagcatg ataatgggga cgcgcgactt ctcgcgcagt
     3121 tgctggcaca cggtccaccc atccgtgccc ggtatcatca cgtccagcac caccagatgg
     3181 ggttgcgcct cggctgccaa gcgcagaccg ttcctgccgt cgagcgcggt cagcgggcga
     3241 tagcctgcag cctgcaagtg cccggccagt agttcgagca gcgtcgtatc gtcgtcgatc
     3301 agcaagatgg tctgctgcgc catcctcagc tcgccaccgg gagcgcaaaa gagaagatcg
     3361 cgccgccgtc gggatgattc tcagcccaga tgcgcccgcc ctgcgcttcc accagttggc
     3421 ggcagatgta cagcccaagc ccgtaaccgt aggccgcctg cgcgtcgctg ccgtcggtgc
     3481 gataaaactt ctgaaaaacg cgatcgagat cggacggcgg aagaccggag ccgaagtcac
     3541 gcaccgagac gatcacttca atctcatcgg cctgcacgtc gatgacgacg tctttccccg
     3601 gcggcgaata cttgtccgcg ttgtcgagca gattggcgat cacctctgcc gtgcggtcgc
     3661 gatcggcaaa ggcgagcggc aggccgggct tcgtcggcaa gcggaagggt cgccgcgtcg
     3721 tgcgggcgcg gatctgttcc gtgacttggc gcacgacagg cagcaccgaa atcggttcgg
     3781 gctgcagcac gacgtcgccg gcttcaatac gcgccgcgct caacacgtct cgcaccaggc
     3841 gatcgagtcg ccggacttgc tggttcagga cactcaacat gcgcgagcac gtcgcgttca
     3901 tcacgctgca gttggcctcg atgtgctcga ccgcgccgtg catgttggtg agcggcgcac
     3961 ggagttgatg cgacacgagc gagacaaatt ccgaccgcat gcggtcgagc ttttgcagat
     4021 cttcgttcgc gcgggcgagt tcttcgactt tctcggccag ttcgcgtgtc cgctcggcga
     4081 cctgccggtt caggctggcg ttgagccggc gaatttcctc ttcgcgtcgt ttgcgatcgg
     4141 taatgtcccg caggatgatc gacatggcag ggcgccgacc ggattcgcct gggaggcgcg
     4201 tcgccgtcaa ttccacggcg atcgtgcgcc ccgtggcatc gcgacaggtc gtctcatgcc
     4261 cgcgcacgaa tcccgcttcc cggacggcct cgaccagcca cctgaactcg acttcggcgg
     4321 tctcgccttc ccggtacaga tccgtgaacg gccgatcccg tatctcgtgc gccgaatagc
     4381 cgaagatgag ttcggcgcca cgattccagg atgcgatgcg cccctggtcg tcgagactga
     4441 gaatggcatc ggccgaggcg gatgtgatcg acgccagacg ctgttcgctg gcctgagcct
     4501 gcctctcggc gcgctccttt tcgtcgagcc agcggccgat gagtgtcagc atccagaacg
     4561 ccagcaaagg accggccgtg ctgtaaaaaa ggatctcgat ggcgaagtgc gccgggtcgc
     4621 cataggtgtc gtgtacccag cgtgcccaca gtagttggta cagtgtcgcc agcgcggaga
     4681 agccaagcgg caatacccag cgaagcttgg cgattcgctg tctgagagca gggcctgtca
     4741 caaacataaa cactcctcct cgcctgtaac gccgtcgaga gtcaggtctg caggtgatcg
     4801 cgaagtttct gaatggcgcg atccagcgcc tcttctgttt cgtgcagtgc gccggcggtt
     4861 tcggtccgaa cctcgggcag cgtggatttg agcgaggcca gcagcgcgct ggcggcgaac
     4921 agcgtctgca gcgcctcgtc gctcagctcg cggccgatgc gctcgctctc gcgagcgtca
     4981 gccagcacct gcgcctgaaa ctcggacgta tcccgctcgt ccagccagcg gccgacgaaa
     5041 tcgaggagaa ggaaggcgag ggcaggccct accgtgccat acaccaggat ctcggcgacg
     5101 aagtgatgat cctcgcccaa gacatggtgg atccagcgcg ccgggccgat ctcgtagatg
     5161 acgaccagaa gcacgagcgc cgccggtacc agcttctgaa gcaaacgaaa gcgccgccgg
     5221 aaagcgacgt gcgacagccg tcgctcgcgc ctcgggtttt gcatacggcc gagcgtcagg
     5281 cgtttcgaga aatcagacat tctgctttcc gcatccgacc tcacgtgcga cgcacttcgc
     5341 aggtgcgtcg cacgtgactg cccgctcact gcacccaatt cgacaggacg accacttctt
     5401 tctggggatt cgccggatcg ttcgtcggca ggtgcaggcg gaagtcgtgc atgccgccca
     5461 tgttgccgtg catcatgaac tgcatcgaca cccacgtgcg ctcgcccggg ttgatgacca
     5521 tcgaaccgat ggtcggggtg ggcggtcagc atccctccac gacggtgacg tatggctgct
     5581 ctgtgaagcg cagcgtctgg tcgccggcgt tcgccagctc gaagttgacg gtcacccact
     5641 cgcccagccg cacggtcccc agatcgactt tctccttgtc gaccttgagc gccggcgcgc
     5701 cgctcacttc gatggccgcc ggcgtgccgc tcacttcgac ggtcgccggc gggcttgccg
     5761 gcgcgccgcc gccgaagatc agcagcgcgg cgatagcgac gagcgccacg ccgccggcga
     5821 tgatcagcag cggagccttc gattgagaag tcttgtgcct ggaagcgcct ggcttcctct
     5881 tcttcttttg actcatccgt tcccctccag gtcaaattgg aatgtccaca gatagctgat
     5941 gacggaccac agttgatcct cggtgaaaat cggtccccag tatggcatgc ctgtgcccat
     6001 gccgccacgc acgatcttgc cgtgcagcag cgccgggctt gcgccgagca tcgacgaggc
     6061 gtcggtgaag tcgacgggcg ccgtcgtgtc gtgccgctgc atgtcgccag cgcccgtctc
     6121 tccggtcaac gatgcggcca tcacgccgtc gcctgcgccg ttttcgccgt gacacgcggc
     6181 gcagttttgg gcgaacagtt tcttccccgt ttcaagcgcc tgcgcagttg tattcgatcg
     6241 ccacacgtct gcgacgagat cccacacctg cccctcggtt aagccttgcg tgaacggctc
     6301 gtcgcgcagt tccttccagg catcggccgg gctgtgtgtg cggtagaact cggccgtcag
     6361 gtaattgacg gggatggcga tcccgagcgc cgcgccgcgc tgtgccgacg gcgctcgatc
     6421 gggaaggacg tccgtgggat gcggcgcatc gatgtcaatg ccgagcctca cgtacagcgg
     6481 ctgttccact gcccactgat cactgacaga ctgatcactg atcacttcga tcgttccccg
     6541 catacgccag tgattcggcc cgcaccagcg cgtgcagtaa aacgtgacct tgccaggctc
     6601 gtcgaaggtc agcgtcgtct cgatcacctc gccgggtttc acgtcgagcg cgggcaggtc
     6661 gctctggccg atggcgaagc cgtgtattac gtcgtcggag gtcaatcgca ggcggatcgg
     6721 ctcgccggcc ttcgccgtga tgtcggacgg cgtccagcca ccggtctccg acatcgtgcc
     6781 gtgcaattcg atcgtttggc cggcgaccga ttcggccttg ccggcgatga ccgccgcgac
     6841 cggcaggccc acggcgagca cgatgacgat agttcgggcg atacgttcgg cgctcatccg
     6901 atcaacaacc ctagcagccc gatcgtcacg agcaggcaga aggcgataat gggcagcgct
     6961 tgcagcgtag ccgtccgttt gggtaactgc tcgcccgcga tgcggcgcgc cgtcatgctc
     7021 gcccacgcca gcccgcctag tagcgcggta gcctgcaaga caggcacaac ctgcgtgaga
     7081 tacggcgccc acgcgattcc cgccgtgcca agcaggttcc agccctcacc catcgggtcg
     7141 gacagcgcag gccatacgta cgagaggtta gcgagcacga acgaaatgct gaacgcgatc
     7201 cacgccgcga ggccgagcgg cacgagcgcg taggcgaaag ccgtgaacgc cttcttcgac
     7261 ttcattttcg agccggaaag ccgccggccg agatggacgg cgaagagaaa cagtcccggc
     7321 agcgcgacga gcacaccggc gaggaaactc accgcgtaga cgagccactc gaccgatccg
     7381 atggagtatg cggcggcctt cagctcaccc cactgcccga gcatcaccgc cgaatacacg
     7441 acggcgctgc cgagcatgat gaacgccttg aacgcctcgt cgagtttccg cccgctcggc
     7501 ttgagcagat cggccccgaa cgggcgcagg ttgagggcga tgttgtcgtg cgggcacgtg
     7561 cgcaggcact cgacgcacgc gccgcagtaa gtgttcttcg ccagcccgcc ggggaagacc
     7621 tgccaggggc agccgtagcc gccggcgctg ccggtgtagc acgtcttctc ggtgtgcgag
     7681 gcgcacagcg ccgcatcctt ggcgcgcact tcgatcggcg cgagctgcga gtacaggccg
     7741 atgaagccgc ccacggggca cagatagcgg cagaacgcgc gccgctcgaa gatcaggctg
     7801 gtgacgatgg cgaggacgag gaacagcgcg agcacgagcg ccgtcacgcc gggctgtgtg
     7861 agcacgacga ggctgaagag cgcgacggcg acgaacgcgc cgttttgaat ccacatgctg
     7921 cgcaagcgcc ggggccagcg cctgccgagc gagaaccccc ggccctgcgg acccagcacc
     7981 gcgccatttt gcagccactc gccgggcagc gggatcgggc aaatgctgca ccagccgcgc
     8041 ccgaagagcg gcacggcgat cagcatcagc accgcccacc acgcgatcca cacgaacacg
     8101 atgccaaagt tgcggctgcc gaccggcgtg ccggccagcc cggcgacaat ggcgaagacg
     8161 aatcctgcga gcgcgatcac gcgcaccagg aactgcggcc agcggctgcg cagggccggc
     8221 ttcaccagag gaagttcagc cagatcgaat cgtgggtact gtaccatgat ggccggttac
     8281 gtaaggcttt tctcaccacc aagacacgaa gggaccaagg ctttggaata tttgcccgtc
     8341 gtgaagacga atcttcacac ctcttcgtgt cctggtgtct tcgtggtgaa aactgctcct
     8401 aaactcgaat tgtcctattt gcgaaacagc catacgccgg cgaaggtagc cagaaccgcc
     8461 agcccgatcg cgcgccacag caggtcgttc tcgccgacgg tgagcttgcc gatcatgaac
     8521 gggtggagcg cgccgcacgt caccgagcag cggaatcgaa acgcgccggc cttgtcggcg
     8581 gtgaacgtca gtcgcgccgg ttggccggga tcggcggtgg tgttcagatc gtagccatcc
     8641 aggtacaggc cgtggacgac gtccgtcgag gcaagctcga tcgtcacacg gtcgccagga
     8701 ttgaccgcga tctcggcggg cgtgtattca aaactgctcg cctcgacgcg gaagtatcgt
     8761 tcgcccggcg cagcggagag cgccggaatg ggaaccagca ggatggccag gccggcgaga
     8821 atgacgattg gccagcgcca gcggcgcgac accttcgttc gagtcatggt tcccccgctc
     8881 acgactccga actacggcat gtttgaaggg ccgtaccgcg agtaggtctc gtcgacgatg
     8941 gcgcggattt cggagacgct cttgccctgc ctgagcaggc gcatggtgtc ctgcgtgatg
     9001 tccacgcaga tcgagcagcc gagcgcgtgc ggatcgtact tcagcgtgcc gccggcgttc
     9061 gttcctgcca cgtagcaggc ataattcgag gtgtggccca tgtcgccgca gccacaatag
     9121 caggggattt gcttgagcac gtcgggattc gcgacggcga actggtaggc ctgctgcacc
     9181 gcgaccggcg cggtctgcac ttccaccggc atcggggcca ggtcggccat ctgaaaggga
     9241 tgtgcggcag tgtcgccgga tgcagaggcg tcgcatccgg tggccaggcc gccgagcgcc
     9301 agcaggccag tcaccaacag caaaaagaat cggcgttgat agaacatccg tttcccctct
     9361 tgggctttca ttcgtgaaaa tgatacccac gtatgagcca tatttatagt gatattcgtc
     9421 atccaattag ggtgatgggc cacagcccga c
//
LOCUS       DBNHXB010000035         7660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1685265, whole genome shotgun sequence.
ACCESSION   DBNHXB010000035 DBNHXB010000000
VERSION     DBNHXB010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1685265"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1877
                     /locus_tag="AC162G_01435"
     CDS             24..1877
                     /locus_tag="AC162G_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR5074509.1"
                     /translation="MPSDIEIAQAVKLKPILQIAEETGLKPSELELFGPYKAKVHLEV
                     RERLAKRPNGRYIDVTAITPTPLGEGKSTTMVGLSQALGAHLGRKVFTCIRQPSQGPT
                     FGIKGGAAGGGYSQVVPMEDFNLHLTGDIHAVSAANNLLAAAIDTRMLHERAQLDDEK
                     LAKAMTKDGQFAEGQRRRLAKLGVDGKTLSDLSPEDKRRLFRLDIDPNSITWQRVVDI
                     NDRLLRGIQVGLGQEERGNERFTGFDISVASEVMAILGLTTGLEDMRERLGRIVVGTS
                     KSGEAITAEDLGVAGAMTVLMKDAIMPNLMQTLEGTPAFVHAGPFANIAHGNSSIVAD
                     QIALKLVGPDGFVVTESGFGADCGMEKFMDIKCRYSGLIPNVVVIVATIRALKMHGGG
                     PKVVAGRPLDKAYTEENLALLEKGAANLAKHIENARLFGVPAVVAINAFQYDTEAEIE
                     LMRKIAIEAGAEGAYKCTNWMHGGKGAIELAEAVVVAAEKKNHFQFLYPLDIPIKQKI
                     ETIATQIYGADGVDYMPEAEAKIDLYTRLGFDELPINMAKTHLSLTHDPNIKGRPTGW
                     RLPIRDIRASVGAGFLYPLCGAMRTMPGLPTRPVFMDVDLDLESGKVVGLF"
     gene            2027..2875
                     /locus_tag="AC162G_01440"
     CDS             2027..2875
                     /locus_tag="AC162G_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR5074510.1"
                     /translation="MLSEELSVAALPLFEGLSVEDMELIAPLFRRVTFPSGTQVFSKG
                     EEASYLYVLESGEVAICVPLHDGGCMDVATVQRGGIWGWSAALGRTEYTANATCRSET
                     TALAVRGSDLRQLIQTRTHLGNQLVERMAQLARNRSDGLRDQVMKLFAQSNGEKSMER
                     LTLNLPAMYGDHHVTEVRRILSALPGVTKVYASAARQRVDVEYDASQIDPPAIEQALR
                     ARGYTADGQAPMLVSARTKTLTEYAIGPGGIEQFVERVPAWGAPLGACPGFEIRQPGE
                     VHPADR"
     gene            2981..4978
                     /gene="cooS"
                     /locus_tag="AC162G_01445"
     CDS             2981..4978
                     /gene="cooS"
                     /locus_tag="AC162G_01445"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HIR5074511.1"
                     /translation="MARKQRTAEERSIDPASQQLLLRAEELGLETAFSRADAMAACPI
                     GAGVSGLCCKNCFMGPCRITKEGQVGVCGATASTIAARNLARAIAAGSASHSDHGRDV
                     AFTLLAAAEGEAQGYQIRDAAKLRAVARHYGVAPDGKSVEQLAKEVALKAIAEFGQQK
                     GELAFVKRAPKKRQELWRKYGIVPRGIDREVVDVLHRTHMGDDQDAEHILKGALRCAL
                     ADGWGGSMIATELSDVLFGTPAPKIGQVNLGVLKDDEVNVVLHGHEPTLSEMIVAASQ
                     DPELIAYAKSKGANGINLAGICCTSNEILLRQGVASAGNFLNQELALITGAVDAMVVD
                     VQCIMQALPNLAEKFHTKFITTSPKVMISGGMHIEFDEHHALDIARRILRVAIDNYPN
                     RGEVVIPDEAEDLVPGFSHEYINYMLGGSYRASFRPLNDAIMSGRIRGVVADVGCNNP
                     RVTQDKSHTWLVREFLKNDVLVVQTGCGALAAGKMGYLTGEAALEYAGPGLREVCEAT
                     GMPPVLHMGSCVDNTRILTVLTQMAQESGLGDDISDIPAVGLAPEWMSEKALAIGAYA
                     AASGACVIFGIESPVEYSQEVTRVMDKVWEKELGGRMIFVSDDETMFRETLAHIDKKR
                     AALGLPEYDPNRFGRSGDWRMEELVALPLETQVEALYGGVR"
     gene            4975..7254
                     /gene="acsB"
                     /locus_tag="AC162G_01450"
     CDS             4975..7254
                     /gene="acsB"
                     /locus_tag="AC162G_01450"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HIR5074512.1"
                     /translation="MSRYIATRAIRGANYVVNEFEMMLGQALVEKGPETPVAFPNTAY
                     NLPLILGMTGRPVEKLGDLQPVLDTAKGLLHPVPPDRRWTPYLGETLDAGMATLLAEE
                     GIEAVRFVYGQQPEPLAGFKLAGGTSFTSPDFAGNGDGRLNGPIDDIQLRSWGIQLVD
                     GRMPGFAAIVGCARSNEVAVKIVRELQRRNILCFLSGNVNGRSVIHQLMEEGVELGYD
                     TYTVPFGTDTISAIYALGFAVRSALTFGGMKGGMARDILLYNKNRVFAFVLALGEVDD
                     LKYATAAGAINFGFPVIADTVIPQILPTGITTYEHVISMPFNEIEGKDDLERAEKLVQ
                     KCIEVRGVKVKISEVPVPVPYGSAFEGEVVRKQDMRLEFGGKKARCFEYLRMLDFDAV
                     QDGKIEVVGPDFSHVPEGEAMNMGIVVEVAGRQMQKDFEPVLERQIHHFVNGASGIQH
                     IGQRDITWIRISKTAAEKGFSLRHFGDILHARFHEDFGAIVDKVQVTIYSDPEQFDQW
                     LVTARAAYDERNVRMGSLTDEAVDEFYSCTLCQSFAPNHVCIVTPERLGLCGAYNWLD
                     CKASYQINPTGPNQPIKKGGAIDPEKGYWQGSNDFVEGASHQQVQTVAIYSIMENPMT
                     ACGCFECIAMVIPEANGVMIVSREDTSMTPAGMTFSTLAGMAGGGLQTPGIMGHGKFY
                     ILSPKFLSADGGFKRVVWMSSILKEQMAEQLKKVSEREGDPDLIERICDERIATDVEG
                     LVAYVTEKGHPALEMPSLL"
     gene            7260..7634
                     /locus_tag="AC162G_01455"
     CDS             7260..7634
                     /locus_tag="AC162G_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5074513.1"
                     /translation="MTTLLVRDVMTIGVPVCRDAETCGDVEARLARQATEAEVVGVLD
                     EDGSACGWISRERLRQKSTHTVGEVMEEDIPTVPPNIPAEAAAQIMRDRGVEYLFLMH
                     DWPGEPRPSAVISLKAIEQRIG"
BASE COUNT         1467 a         2372 c         2594 g         1227 t
ORIGIN      
        1 cccgcctcaa gttgctcagc cccgtgccga gcgacatcga gatcgcgcag gccgtcaagc
       61 tcaagccgat cctgcaaata gcagaagaga ccggtttgaa gcccagcgag ctggagctgt
      121 tcgggccgta caaggccaaa gtgcatctgg aggtgcgcga gcgactcgcc aaacgcccaa
      181 acggtaggta catcgacgtg acggcgatca cgcccacacc gctcggcgag ggcaaatcga
      241 ccaccatggt tggcctgagt caggcgttgg gagcgcacct gggtaggaaa gtcttcacct
      301 gcatccgcca gccttcgcaa ggcccgacgt tcgggatcaa gggcggtgcg gccggcggcg
      361 gctacagcca ggttgtgccc atggaggatt tcaacctgca cctgacgggc gacatccacg
      421 ccgtcagcgc ggcgaacaac ctgctggctg ccgcgatcga cacgcgcatg ctgcacgagc
      481 gggcacagtt ggacgacgag aagctggcga aggcgatgac gaaagacggc cagttcgccg
      541 aggggcagcg acgccgcttg gcgaagctcg gcgtcgacgg caagacgctc tccgacctct
      601 cgccggaaga caagcgccgg ctgttccgcc tggacatcga tcccaacagc atcacctggc
      661 agcgcgtcgt cgacatcaac gatcgcctgc tgcgcggcat ccaggtcggc ctcggccagg
      721 aggaaagggg taacgagcgc ttcaccggct tcgacatctc ggttgccagt gaagtgatgg
      781 ccatcttggg cctcaccacc ggcctggagg atatgcgcga gcggctgggc cgcatcgtcg
      841 tcggcacgag caagtcgggc gaagcgatca ccgccgaaga tctgggcgtg gccggagcga
      901 tgaccgtgct gatgaaagac gcgatcatgc ctaacctgat gcaaacgctg gagggcacgc
      961 cggccttcgt gcacgccggg ccgttcgcca acatcgcgca cggcaactcg tcgatcgtcg
     1021 ccgaccagat cgcgctcaag ctggtcggcc ccgacggctt cgtcgtcacc gagtcgggct
     1081 tcggcgcgga ctgcggtatg gaaaagttca tggatatcaa gtgccgctat tcggggctga
     1141 tcccgaacgt ggtcgtcatc gtggcgacga tccgcgcgct gaagatgcac ggcggcgggc
     1201 cgaaggtcgt cgccgggagg ccgctcgaca aggcttacac ggaagagaat ctcgcgctgc
     1261 tggagaaagg cgcggccaac ctggccaagc acattgagaa cgccaggctg ttcggcgtgc
     1321 cggctgtcgt cgccatcaac gcgttccagt atgacaccga agccgagatt gagctgatgc
     1381 gcaagatcgc gatcgaggct ggagccgagg gtgcttacaa gtgcacgaac tggatgcacg
     1441 gcggcaaagg cgcgatcgag ctggccgagg ccgtcgtcgt ggcggcggag aagaagaacc
     1501 acttccaatt cctttacccg ctcgacatac cgatcaagca gaagatcgaa acgatcgcga
     1561 cccagatcta cggcgccgac ggtgtggatt acatgccaga agccgaggcg aagatcgatc
     1621 tgtacacgcg gctgggtttc gacgaattgc cgatcaacat ggcgaagacg cacctcagcc
     1681 tgacgcacga tccgaacatc aagggccggc cgaccggttg gcgcctgccg atccgcgaca
     1741 ttcgggcgag cgtcggcgcg ggcttcctgt atccgctgtg cggcgcgatg cgcacgatgc
     1801 ctggcctgcc gacgcggccg gtgttcatgg acgtcgatct tgacctggag agcggcaagg
     1861 tcgtcgggct gttctaggga cgggggcggg cctcggctcg ccccttgtca gttgggagta
     1921 gggagttaga gttgggagtt agagttggga gttagagtta ggagttagag ttgggagttt
     1981 tgctggcggt tcacgcatcc ttcgacaggc tcaggacaag attgccatgt tgtcggaaga
     2041 attgagcgta gctgcccttc cgttgtttga gggcctcagc gttgaagata tggagttgat
     2101 cgccccgctg ttcaggcggg tgacgttccc ttccggcacg caagtctttt cgaaaggcga
     2161 ggaagccagc tacctgtatg tgctcgaatc cggcgaagtc gccatctgcg tgcccctgca
     2221 cgacggcggc tgtatggacg tcgccaccgt tcagcgcggc ggcatttggg gctggtcggc
     2281 ggcgctgggg cggacggagt atacggcgaa cgccacgtgc cgcagcgaga cgactgccct
     2341 ggccgttcgg ggcagcgacc tgcgccagtt gatacaaaca cggacccatc tcggcaatca
     2401 gttggtggag cgcatggctc aactggccag gaatcgctcc gatggccttc gcgatcaggt
     2461 catgaagcta ttcgcgcaat caaacgggga gaaatccatg gaaaggctta ccttgaactt
     2521 accggcaatg tacggcgatc accatgtgac ggaggtgcgg cgcatcctga gcgcgcttcc
     2581 cggcgtgacg aaggtgtacg ccagcgccgc gcggcagcgc gtcgacgtgg agtacgacgc
     2641 cagccagatc gatccgccgg cgatcgagca ggcgctgcgc gcgcgcggat atacggcaga
     2701 cgggcaggcg ccgatgctgg tctcggcgcg cacgaagacg ctgaccgaat acgcgatcgg
     2761 tcccggcggt attgagcagt tcgtcgagcg cgtgccggcc tggggcgcgc cgctcggcgc
     2821 gtgtcccggc ttcgagattc gccagccggg tgaggtgcat ccggccgatc ggtaactacg
     2881 gatgacacgg atgacatgga cttctcagcc atccgtgcca tctgtgtaat ccgtggttag
     2941 aacacacatt actactctaa cagcaaacga gggagacagc atggctagaa aacaacgaac
     3001 cgccgaagaa cgatcgatcg accccgcgtc ccagcagttg ctgctgcggg ccgaggaatt
     3061 gggcctggaa acggccttca gccgggccga cgcgatggct gcctgtccaa tcggcgccgg
     3121 cgtgtcgggc ctgtgctgca agaactgctt catgggcccg tgccggatca ccaaggaagg
     3181 ccaggtcggc gtgtgcggcg cgaccgcgtc gacgatcgcc gcgcgcaacc tggcgcgcgc
     3241 gattgccgcc ggatcggcgt cgcactcgga ccacgggcgc gacgtcgcct tcacgctgct
     3301 ggcagccgcc gaaggcgagg cacaaggcta tcagatccgc gacgccgcca agctgcgcgc
     3361 cgtcgcacgc cactacggcg tggcgccgga cggcaagtcc gtcgagcagc tcgcgaagga
     3421 agtcgcgctc aaggcaattg ccgagttcgg ccagcaaaaa ggcgagctgg ccttcgtcaa
     3481 gcgcgcgccg aagaagcgcc aggagctgtg gcgcaagtac ggcatcgtcc cgcgcggcat
     3541 cgaccgcgaa gtcgtcgacg tgctgcaccg cacgcacatg ggcgacgacc aggacgccga
     3601 gcacatcctg aagggcgcgc tgcgctgcgc gctcgccgac ggctggggcg ggtcgatgat
     3661 cgccaccgag ctatccgacg tgctgttcgg cacgcctgcg ccgaagatcg ggcaggtcaa
     3721 tctcggcgtg ctgaaagacg acgaggtcaa cgtcgtgctc catggccacg agccgacgct
     3781 gtccgagatg atcgtggcgg cctcccagga tccggagctg atcgcgtacg cgaagagcaa
     3841 gggcgcgaac ggcatcaacc tggccggcat ctgctgcacc tcgaacgaga tcctgctgcg
     3901 gcagggcgtg gcttcggcag gcaacttcct gaaccaggag ctggcgctga tcaccggcgc
     3961 ggtcgacgcc atggtcgtcg acgtgcagtg catcatgcag gcgctgccca acctggccga
     4021 gaagttccat accaagttca tcaccacctc gccgaaggtg atgatttccg gcggcatgca
     4081 catcgagttc gacgagcatc acgcgctcga cattgcgcgc cggattttgc gcgtcgccat
     4141 cgacaactat cccaaccgcg gcgaagtcgt gatcccggac gaggccgaag acctggtgcc
     4201 gggcttctcg cacgagtaca tcaactacat gctcggcggc tcgtaccggg cttcgttccg
     4261 cccgctcaac gacgcgatca tgtccgggcg cattcgcggc gtcgtcgccg acgtgggctg
     4321 caacaacccg cgcgtcacgc aggacaagtc gcacacctgg ctcgtgcgcg agttcctgaa
     4381 gaacgacgtg ctggtcgtgc agacgggctg cggcgcgctg gccgccggca agatgggcta
     4441 cctgaccggc gaggcggcgc tcgaatacgc cggtcccggc ctgcgcgaag tgtgtgaggc
     4501 gacgggtatg ccgcccgtgc tgcacatggg ctcgtgcgtc gacaacacgc gaatcctcac
     4561 cgtgctgaca cagatggcgc aggagagcgg ccttggcgac gacatctccg acatcccggc
     4621 cgtcggcctg gcgccggaat ggatgagcga gaaggcgctg gcgatcggcg cgtatgccgc
     4681 ggcgtccggc gcgtgcgtca tcttcggcat cgaatcgccg gtcgaataca gccaggaagt
     4741 gacgcgggtc atggacaagg tgtgggagaa agagctgggc ggccggatga tcttcgtgag
     4801 cgacgacgag acgatgttcc gcgagacgct ggcgcacatc gacaagaagc gcgccgcgct
     4861 cggcttgccc gaatacgatc ccaatcgctt cggcaggagc ggcgactggc gcatggaaga
     4921 gttggtcgcc ctgccgctcg aaacacaggt tgaagcgctt tacggaggtg tcagatgagc
     4981 cggtacatcg ccacgcgcgc gattcgcggc gcgaactacg tcgtcaacga gttcgagatg
     5041 atgctgggcc aggccctggt cgagaagggg ccggagacgc cggtcgcgtt cccgaacacc
     5101 gcgtacaacc tgccgctgat cctgggcatg accgggcgcc cggtcgagaa actcggcgat
     5161 ctgcagccgg tgctcgatac ggcaaaaggt ctgctgcatc ccgtcccgcc cgaccggcgg
     5221 tggacgccct acctgggcga gacgctcgac gccggcatgg cgacgctcct ggccgaagag
     5281 ggcatcgagg ccgtgcgctt cgtctacggt cagcagcccg agccgctcgc aggattcaag
     5341 ttggcgggcg gcacgtcgtt caccagtccc gacttcgccg gaaacggcga cgggcgtctg
     5401 aacgggccga tcgacgacat tcagctccgc tcgtggggca tccagctggt cgacgggcgc
     5461 atgcccggct tcgccgcgat tgtcggctgc gccaggtcga acgaagtggc ggtgaagatc
     5521 gtgcgcgagc tgcagcgacg caacatccta tgcttcctgt ccggcaacgt caacggccgg
     5581 agtgtgatcc atcaactgat ggaagaaggc gtcgagctcg gctacgacac gtacaccgtg
     5641 cccttcggca cggacacgat cagcgcgatc tacgcgctcg gcttcgcggt gcgctcggcg
     5701 ctgaccttcg gcggcatgaa gggcggcatg gcgcgcgaca tcctgctcta caacaaaaac
     5761 cgcgtgttcg ccttcgtgct ggcgctgggc gaggtggacg acttgaagta cgccacggcg
     5821 gcgggcgcga tcaacttcgg cttcccggtg atcgccgaca cggtcattcc ccagatcctg
     5881 ccgaccggca tcacgacgta cgagcacgtc atttccatgc cgttcaacga gatcgaaggc
     5941 aaggacgacc tggaacgcgc cgagaagctc gtgcagaagt gcatcgaggt gcgcggcgtc
     6001 aaggtcaaga tctccgaagt gccggtgccg gttccgtacg gatcggcctt cgaaggcgaa
     6061 gtcgtgcgca agcaggacat gcgtctcgag ttcggcggca agaaggccag gtgcttcgag
     6121 tatctgcgga tgctcgactt cgacgcggtg caggacggca agatcgaggt cgtcggcccg
     6181 gacttcagtc acgtgcctga aggcgaggcg atgaacatgg gtatcgtcgt cgaggtcgcc
     6241 gggcgccaga tgcagaagga cttcgagccg gtgctcgagc ggcaaatcca ccacttcgtc
     6301 aacggcgcgt cgggtatcca gcatatcggc cagcgcgaca tcacgtggat ccgcatcagc
     6361 aagaccgccg ccgaaaaggg tttcagcctg cgccacttcg gcgacatcct gcacgcgcgc
     6421 ttccacgagg acttcggcgc gatcgtcgac aaggtgcagg tgacgatcta ctccgacccg
     6481 gagcagttcg accagtggct ggtcaccgcg cgcgccgcct acgacgagcg caacgtgcgc
     6541 atgggcagcc tgacggacga ggcggtcgac gagttctact cctgcacgct gtgccagtcg
     6601 ttcgcgccca accatgtgtg tatcgtcacg ccggagcggc tcggcctgtg cggcgcgtac
     6661 aactggctcg actgcaaggc gtcgtaccag atcaacccga ccggcccgaa ccagccgatc
     6721 aagaagggcg gcgcaatcga tccggagaag ggctactggc aaggctcgaa cgacttcgtc
     6781 gagggcgcgt cgcaccagca ggtgcagacc gtcgccatct actcgatcat ggagaacccc
     6841 atgaccgcct gcggctgctt cgagtgcatc gccatggtca tccccgaggc gaacggcgtg
     6901 atgatcgtca gccgcgaaga cacgagcatg acgccggcgg gcatgacctt ctcgacgctg
     6961 gcgggcatgg ccggcggcgg cttgcagacg ccgggcatca tgggccacgg caagttctac
     7021 atcctcagcc cgaagttcct atcggcggac ggcggcttca agcgcgtggt gtggatgtcg
     7081 tcgatcctga aggagcaaat ggcggagcag ttgaagaagg tctccgagcg cgagggcgac
     7141 cctgacctga tcgagcgaat ttgcgacgag cgcatcgcta cggatgtcga gggcctggtg
     7201 gcgtacgtca ccgagaaggg gcatccggcg ctggagatgc cgtcgctgtt gtaacgccga
     7261 tgacgaccct ccttgtccgc gacgtgatga ccatcggcgt tccggtctgc cgagacgccg
     7321 agacgtgcgg cgacgtcgaa gcgcgcctgg cgcggcaggc gactgaggcc gaggtggtcg
     7381 gggtgctgga cgaggacggc tcggcctgcg gctggatctc gcgcgagagg ctgcggcaga
     7441 agtccacgca cacggtcggc gaagtgatgg aagaagacat accgaccgtg ccgcccaaca
     7501 ttccggccga ggcagccgcc cagatcatgc gcgatcgcgg cgtcgagtat ctctttctga
     7561 tgcacgactg gcccggcgag ccgcgcccat cggcggtcat ctcactgaaa gccatagagc
     7621 agcggatcgg gtgaattcag cggatgtatg gacagggagc
//
LOCUS       DBNHXB010000036         9035 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2524502, whole genome shotgun sequence.
ACCESSION   DBNHXB010000036 DBNHXB010000000
VERSION     DBNHXB010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9035)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9035)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9035
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2524502"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="AC162G_01460"
     CDS             <1..673
                     /locus_tag="AC162G_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HIR5074514.1"
                     /translation="LAHVYLPMVARMGVKAECRLDAWGFYPIGGGQITADVYGKRRTF
                     APLSVLERGDLKRIRGAAVACNLPAHIAQRIANRARNVLVDAGLPVDVTPKRERGAGP
                     GAGLFLVAEYEHSLAGFSALGAKGKPSEQVADEACRDLLAHHAGGAPVDGHLADHLLL
                     PMALAAGRSEFRAACVTQHLLTNAHIIEQFIPARIEVGGSEEEPGTIVIARRGPATPD
                     LLAAG"
     gene            complement(712..1326)
                     /locus_tag="AC162G_01465"
     CDS             complement(712..1326)
                     /locus_tag="AC162G_01465"
                     /inference="COORDINATES: protein motif:HMM:NF025052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop nucleotide/nucleoside kinase family
                     protein"
                     /protein_id="HIR5074515.1"
                     /translation="MGEAARSHTIDLQLAVDQIESLLSIPDDRALPPLLIVLVGLPGT
                     GKSTLARALAQAMPAVVVESDFVRKQLFDSPTHSGQESQLVHSVCHAVIRRLLRRGVS
                     AVSDATNLIESQREFLYHLADQCDARLLVVRVAASEPVIRQRLGQRQVARSPHDWSDA
                     DWSVYLRMQQRQDAIGRPFVTVKMDGDVAEAVGKVLRAARQLRR"
     gene            complement(1500..1829)
                     /locus_tag="AC162G_01470"
     CDS             complement(1500..1829)
                     /locus_tag="AC162G_01470"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR5074516.1"
                     /translation="MKRGELYRVPHPSARDPKKFRVFVVVSRQVLIDSRFSTVVCAPI
                     YSTHDGLSTQVLVGVDEGLKHESSIHCDELVSLPKSTLTHYVGTLSPEKIEALNQALC
                     IALDIPD"
     gene            complement(1826..2056)
                     /locus_tag="AC162G_01475"
     CDS             complement(1826..2056)
                     /locus_tag="AC162G_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HIR5074517.1"
                     /translation="MKVKTSITLSEDLLAAIDKRAGQFKNRSDFIETALRAFITQMAR
                     DEQNARDLAIINQQADRLNREAVDVLAYQVDL"
     gene            2218..2853
                     /gene="mobA"
                     /locus_tag="AC162G_01480"
     CDS             2218..2853
                     /gene="mobA"
                     /locus_tag="AC162G_01480"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HIR5074518.1"
                     /translation="MATSATGIILAGGQSRRMGTDKAFVLFDERTLIERTMDALRQVC
                     GEIVVVANDIAPYTRFGARVVPDTIPDFGPLAGLHAGLEAMRTDLGVAVAVDMPFLNP
                     ALLRAMIDAAEGWDAVIPALASEVHAADVKRHRAKDLDIHPLHAVYHRTCLPRIQAAI
                     DRDDRRLNAFLPDIRVRYLYADEMRAYDPDLRSLINLNTPDDLERASRQRA"
     gene            2966..4273
                     /locus_tag="AC162G_01485"
     CDS             2966..4273
                     /locus_tag="AC162G_01485"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR5074519.1"
                     /translation="MSVVNAGELERRTRELHALTEVAKTLTAPLDLPTLLGAVMDKLT
                     DVLGPAELGALMLWDESAGIFRPEATFGYDLAVMRDMGLRAGESITGKVHDAGTASLF
                     ATPEQVAQAMADMRPANRAAMARALGSDVLPRGAVAAPLQVGDHRYGVLVLETLHGPA
                     EFSASDLPLVQTLADLIALAIDRERLEAEAIAIRDAQQADRLRSEVMATLSHELRTPL
                     GSIKGYATALLLDEVTWPDEKRDEFLRLIDEECDNLQAMISDILDSSLIDVGQLVIEK
                     QPVRLPHLAREVADDMQRRTDGHRLIVDFPANFPIVDADPRRIRQVLRNIVDNAIKYS
                     PDGGLIIIRGEVEHDAQRVVVSISDQGVGISPEDLIPLFEKYFRVKAPTGYHVPGTGL
                     GLPVARAIVEAHGGRIWAESKVGQGTTLHFSLPTTGLSAATGE"
     gene            4332..5042
                     /locus_tag="AC162G_01490"
     CDS             4332..5042
                     /locus_tag="AC162G_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5074520.1"
                     /translation="MTKPRILVVDDEPKLVRLVHEVLTATGYAVLSSGSGEAAIEMVA
                     LEQPDLILLDIVLAGAIDGYEVARRVREFSGVPIIMLTAKARESDLLRGFDVGADDYL
                     TKPFSSKELLVRVRAVLKRARQEPVARTEAEIVCGNLRIDLARRRVTVDDREVHLTRT
                     EYNLLHELARHRDQVLLHAQLLTAVWGPEYRDDLDYLRAYIRYLRQKLEADPSNPKLI
                     VTHPGVGYMLACSDNPPS"
     gene            5253..5732
                     /locus_tag="AC162G_01495"
     CDS             5253..5732
                     /locus_tag="AC162G_01495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE family
                     protein"
                     /protein_id="HIR5074521.1"
                     /translation="MHITIDEINAVIDREARGDRSLIAALEAIQERYRYLPPEALILA
                     SERLGVPLSQTYSVATFYNAFSLVPKGKHCLNVCTGTACHVRGSLQVLNRLETKLGVK
                     AGHTTRDRVFTLETVNCLGACALGPIVVTDGEYSGQMTPQKADILLKRILRAEAEHG"
     gene            5725..7581
                     /locus_tag="AC162G_01500"
     CDS             5725..7581
                     /locus_tag="AC162G_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HIR5074522.1"
                     /translation="MARLNTLVDLDARRAQLTAGEPDLPCITVCGGTGCRAYGSEKVW
                     TAFRDEIARQNIDAALHFDVKVTGCHGFCEKGPLVVIRPQAIFYTHAMVEDVPEIVAE
                     TIVGGRVVERLLYRDPRSGDNIEREGDVPFYRHQRRVILDLNGDLDPTQIDEYVERGG
                     YAALAKVLTSMSPQQVIAEVKASGLRGRGGAGFPTGAKWELCRKQPGDTKVVICNADE
                     GDPGAYMDRSIIEGNPHRVIEGMIIGAYAIGAHQGYVYIRAEYPLAVKTLRTAARQAE
                     EAGLLGKNILGSGFDFSLGIRLGAGAFVCGEETALMASIEGRVGEPRPRPPYPAESGL
                     WGWPSNINNVKTWASVPIIINRGADWYAGIGTPKSKGTMIFSIVGKINNTGLVEVPMG
                     ISLRTLIFDIGGGIPNGKAFKAAQIGGPSGGCLPAEHLDVPIDYESLTALGAIVGSGG
                     LVVADEDTCMVDFARYFMAFTQEESCGKCVPCRAGTKAMLATLERICAGEGQPGDIEY
                     LQEMGEHVKHTSLCGLGQTAPNPVLSTLRYFRDEYEAHIFDRTCPAKVCKGLITFSVI
                     PEKCTGCMVCLRNCAVNAITGAKQVVHTIDAGLCTRCGMCKSVCKFDAIRVS"
     gene            7590..8282
                     /locus_tag="AC162G_01505"
     CDS             7590..8282
                     /locus_tag="AC162G_01505"
                     /inference="COORDINATES: protein motif:HMM:NF024900.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR5074523.1"
                     /translation="MVHLTINDQPVEVAEGRTLLEACREHNIHVPTLCYHPALEPYGA
                     CRLCMVEVSHPPREPRLVAACAYPCEEGAAVKTDTETVRHSRRLTAELLAGVARTPEI
                     TTLAESLGVQEVRYTMPDGDACVLCGLCVRACREIVGVSAISLIRRGIAKKVSPPFEI
                     ASSTCIGCGTCVLICPTGALKLSDVTGYRGVHPSHSEYSRVYCQVCGDVDLSPRFLED
                     VESLLAGAQDEG"
     gene            8287..>9035
                     /locus_tag="AC162G_01510"
     CDS             8287..>9035
                     /locus_tag="AC162G_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074524.1"
                     /translation="MSEESSSPSAARHPTSAIRLGVVICDCGKAIARRLDTESLRRRA
                     AALPDVVYTAREAYPCSKDGQARLRQAIRDQGLNRVLVAGCTPRLVEKLFREAGRAAG
                     LDDACVEVTDIREQCAYVHDGDAQTMTEKAHNLIEMGVERLAAISLPQVHTGRVVKSA
                     MVMDGGLSGLTVALSLADEGIDVTLVEPGGALGDLQPPLSSPPSDGGNRGGAGARRLI
                     AERIEAVSRHPRIRTLLNARVTDVTGRPGDY"
BASE COUNT         1680 a         2908 c         2864 g         1583 t
ORIGIN      
        1 cctggcgcac gtgtacctgc cgatggtggc gcgcatgggg gtgaaagcgg aatgccgcct
       61 ggatgcctgg gggttttatc cgattggagg aggccagatc acggccgacg tttacggcaa
      121 gcgcagaaca ttcgcaccgc tttcagtcct cgagcgtgga gacttgaagc gaatccgagg
      181 cgcggccgtc gcctgcaacc tgccggctca catcgcccaa cgcatcgcta atcgagcacg
      241 gaatgtgctg gtcgacgccg gtctgcctgt cgacgtcacg ccgaagcgcg agcgaggcgc
      301 cggtccaggc gcgggcttgt tcctggtagc agagtacgag cacagcctgg ccggcttctc
      361 ggcgctcggg gccaagggta agccgtcgga gcaggtcgcc gacgaagcct gccgcgatct
      421 gctcgcgcat cacgccggtg gcgcgccggt cgatgggcac ctggccgatc atctgctcct
      481 gccgatggcg ctggcggcgg gccgctcgga gttccgcgcc gcctgcgtga cccagcacct
      541 gctgaccaac gcgcacatca tcgagcagtt catcccggcg cgcatcgagg ttggcggcag
      601 cgaggaagaa cctggaacaa tcgttatcgc tcgtcgggga cctgcgaccc ccgacctcct
      661 ggctgccggg tgacacaccc ggcgggaaca atataccgcc aagccgttgc atcagcggcg
      721 tagctgtcgc gcggcgcgca gcaccttgcc gacggcttcg gcaacatccc catccatctt
      781 caccgtcacg aacgggcggc cgatcgcgtc ctgccgctgc tgcatgcgca ggtaaacgct
      841 ccagtccgca tccgaccaat cgtgcggaga gcgggccacc tggcgctgcc cgagccgctg
      901 ccggatgaca ggctccgatg cggcgacgcg cacgacgagc aggcgcgcgt cacattggtc
      961 ggcgaggtga tacaggaact cgcgctgcga ttcgatcagg ttggtcgcgt cgctgacggc
     1021 cgaaacgccg cgccgcagca gccgccggat cacggcgtgg catacgctat gcacgagctg
     1081 gctctcctgg ccgctgtgag tcggcgagtc gaacagttgc ttgcgcacga aatccgactc
     1141 gacgacgact gccggcatag cctgcgccag cgcacgcgcc agcgtcgact tgccggtgcc
     1201 cggcagaccg acgagcacga tcagaagagg cggtagcgca cggtcgtccg ggatgctcaa
     1261 cagcgattca atctggtcga cggccagttg taaatctatc gtgtgtgatc tggctgcctc
     1321 gcccacgact taacccgcat acctctgagc ggcgaaatac tcgtggagca acgccgcgaa
     1381 tccgtaagcg atcggcaatg acgcaacatc tccgaactga ttctcaacaa tgaccgcaac
     1441 tactggtatg ccagccatca gccgatcccc gattgcctgc cgtttctttg ttctcgacat
     1501 taatctggga tgtccagagc aatgcataag gcttgattga gagcctcgat tttctccggg
     1561 gaaagcgtgc ctacgtaatg cgtcagcgtg gacttgggca gactcaccag ctcgtcgcag
     1621 tgaatactgc tttcatgttt taagccttca tctacgccga ccaggacttg cgtggataaa
     1681 ccgtcatgtg tcgagtaaat cggggcgcag accaccgtcg agaagcgcga atcaatcagc
     1741 acttgcctgc tgacgacgac gaagaccctg aatttcttgg ggtctcgggc ggaaggatgg
     1801 gggacgcgat acagctcgcc gcgtttcaca gatcgacctg ataggcgaga acgtcgacag
     1861 cttcccggtt gagtcggtca gcctgctggt tgatgatagc cagatccctg gcattttgtt
     1921 cgtcgcgcgc catctgggta atgaaagccc ggagagccgt ttcgatgaaa tcggaacggt
     1981 ttttgaactg cccggcgcgt ttgtcaatgg ccgccaacaa gtcttcagaa agagtgatag
     2041 aggtcttgac tttcatacta caaatatact ctcattcgga ttacctgtca agcgcttcgc
     2101 tttctatgat ctggagtttg gcaaattccg gaatagaggc tttagccggt tcgattgtcc
     2161 gccacatctt cggctgaagc ctcgattcca gaagaagaac tgcggtagaa taaccatgtg
     2221 gcgacatcgg cgacaggcat catcctggcc ggcggtcaga gccggcggat gggcacagac
     2281 aaagcatttg tcctcttcga cgaacggacg ctgatcgagc gcacgatgga tgcgctgcgg
     2341 caagtatgcg gcgagatcgt cgtcgtggca aacgacatcg cgccttacac gcggttcggc
     2401 gcgcgcgtcg tgcccgacac gattcccgac ttcggcccgc tggccggcct gcacgccggc
     2461 ctggaagcga tgcgcaccga cctgggcgtc gccgtggccg tcgatatgcc gtttctcaac
     2521 ccggcgctgc tgcgcgcgat gatcgatgcc gccgagggct gggacgccgt gattcccgcg
     2581 ctcgcctccg aggtccatgc ggcggacgtg aagcgccatc gcgccaagga cctggatata
     2641 cacccgctgc acgcggtgta tcaccgcaca tgcctgccga ggatccaggc ggccatcgat
     2701 cgtgacgacc ggcggctcaa tgcgttcctg cctgacatcc gggtgcgtta cctctacgcc
     2761 gacgaaatgc gggcctatga tccagacctg cgatcgctga tcaacctgaa cacacccgat
     2821 gatctggagc gggccagtcg ccagcgggcg tgacattcat catgatgaac ccatacaatc
     2881 cctctgttga tctggcgggt aggcttgcgt acaatcgacg tgggtcaacc catggccttg
     2941 aaatccaacc tgccgaggct ctcccgtgtc cgttgtaaat gctggcgaac tcgaacgccg
     3001 cacgcgcgaa ctgcacgccc tgaccgaggt cgccaagacg ctcaccgccc cgctcgacct
     3061 gccgacgctg ctcggcgcgg tgatggataa gctcaccgat gtgctcggcc ctgccgaact
     3121 cggcgcactt atgttgtggg atgagtcggc gggcattttc cggccggaag cgacgttcgg
     3181 ttacgatcta gccgtgatgc gcgacatggg tctgcgcgcg ggcgagtcga tcacgggcaa
     3241 agtgcacgac gcaggcactg caagtctgtt cgcaacgccc gaacaggtcg cgcaggcgat
     3301 ggccgacatg cgcccggcca accgcgcggc gatggcccgt gcgctcggct ccgatgtcct
     3361 gccgcgcggc gcggtagccg cgccgctcca ggttggcgat catcgctacg gcgtgctggt
     3421 gctggaaaca ctgcacggcc cggccgagtt ctccgcaagc gatctgcccc tggtgcaaac
     3481 gctggccgac ttgatcgcac tggcgatcga ccgtgaacgg ttagaagcgg aagccatcgc
     3541 catccgcgac gcgcagcagg ccgatcggct gcgctcagaa gtgatggcca ccctgtcgca
     3601 cgagctgcgc acgccgctcg ggtcgatcaa aggctacgcc accgcgctct tgctggacga
     3661 agtcacctgg cccgacgaga aacgcgacga gttcctgcgt ctgatcgacg aagagtgtga
     3721 caaccttcag gcgatgatca gcgacattct cgactcgtcg ctcatcgacg tgggccagct
     3781 cgtcatcgag aagcagccgg tgcgcctgcc gcacctggca cgcgaggtcg ccgatgacat
     3841 gcagcgtcgc accgatggtc accgcctgat cgtcgacttc ccagcgaact tcccgatcgt
     3901 tgatgccgac ccgcgccgca tccggcaggt gctgcgcaat atcgtcgata acgccataaa
     3961 gtattcgccg gacggcggac tgatcatcat tcggggcgaa gtggagcacg acgcgcagcg
     4021 cgtcgtcgtc agcatctccg atcagggtgt gggtatttcg ccggaggatt tgatcccact
     4081 cttcgagaaa tacttccggg tgaaagcgcc gacgggttat cacgtgcccg gcaccggcct
     4141 gggtctgccg gtggcgcggg ccatcgtcga ggcgcatggc ggccgtatct gggccgagag
     4201 caaggtcggg cagggcacga cgctgcattt ctcgctgccg accacgggat tgagcgcagc
     4261 aaccggcgag tagtcactgt ccagacttcc gaagtctgcg agacttcgga agtctcggtt
     4321 gagatacgcc tatgaccaaa ccacgtatcc tcgtcgtcga cgacgaaccc aagctcgtcc
     4381 ggctggtgca cgaggtgctg accgccaccg gctatgcggt gctgtccagc ggcagcggcg
     4441 aagcggcgat cgagatggtc gcgctcgaac aacccgacct cattctgctg gacatcgtcc
     4501 tcgccggcgc gatcgatgga tacgaagtgg cccgccgcgt gcgcgagttc tccggcgtgc
     4561 cgatcatcat gctcacggcc aaagcccgcg agtccgattt gctgcgcggg ttcgacgtgg
     4621 gcgcggacga ctacctcacc aagccgttca gctctaagga attgctggtg cgcgtgcgtg
     4681 cggtgctcaa gcgagcgcgc caagagccag tcgcacgtac cgaagcggag atcgtctgtg
     4741 gcaatttgcg gatcgacctg gcccgccgcc gcgtcacggt cgacgaccgc gaagtccacc
     4801 tgacccgcac cgaatacaat ctgctgcacg aactcgccag gcaccgcgac caggtgctgc
     4861 tccacgcgca actgctcacc gccgtgtggg ggccggaata ccgcgacgat ctggactacc
     4921 tgcgcgccta cattcgttac ctccgccaga agctcgaggc cgacccctcc aacccgaagc
     4981 tcatcgtgac gcatccaggc gtgggctaca tgctggcctg ctccgacaac ccgccttctt
     5041 gacctctgct ccaatccatc tcagaacctc ccaaaggtcc ggaacctttg ggaggtttcc
     5101 gtttttcacg cgcttttcat gcgcgcctcg tcgttttcta tcgttttctc acatccccgg
     5161 ccctaaactg aagtcgcacg cttccacgta gaacgggcat ccctatgccc gttctggtgc
     5221 gtgcggcaat gaagcaagac gggagtgcac ccatgcacat taccatcgac gaaatcaacg
     5281 ccgtcatcga ccgcgaggcg cgcggtgatc gctcgctcat cgctgcgctg gaagcgatcc
     5341 aggagcgcta tcgctacctt ccccccgagg ccctgatcct cgccagcgaa cgcctgggcg
     5401 tgccgctgtc gcaaacgtac agcgtggcca ccttctacaa cgctttcagt ctcgtgccca
     5461 aaggcaaaca ttgcctgaac gtgtgcacgg gcacagcctg ccacgtgcgt ggcagcctgc
     5521 aggtgctcaa tcggctggag acgaagctgg gcgtcaaggc cggccatact acgcgcgatc
     5581 gggtgttcac gctggaaacc gtcaactgcc tgggcgcgtg cgcactgggg ccgatcgtcg
     5641 tgaccgacgg cgagtactct ggccagatga cgccacagaa agccgacatc ctcctgaaac
     5701 gcattctgag agcagaggcg gagcatggct agactcaaca ccctggtcga cctggacgcc
     5761 cggcgcgccc aactcaccgc cggcgagccg gacctgccgt gcatcaccgt gtgtggcggg
     5821 accggttgcc gcgcgtacgg gagcgagaaa gtctggacgg ctttccgtga cgagatcgcc
     5881 cgacagaaca tcgacgccgc gctccatttc gacgtgaaag tcaccggctg ccacggcttc
     5941 tgcgagaaag gccccctggt cgtcattcgg ccgcaggcga ttttttacac gcacgccatg
     6001 gtcgaagatg tgccggagat cgttgccgag acgatcgtcg gcggccgcgt ggtcgagcgg
     6061 ctgctgtatc gcgatccacg gtccggcgac aatatcgagc gcgagggcga cgtgccgttc
     6121 tacaggcacc agcgccgcgt catcctcgac ctgaacggcg atctcgatcc gacacagatc
     6181 gacgagtacg tcgagcgcgg cggatatgct gcgctggcca aagtgctcac atcgatgtcg
     6241 ccccagcagg tcatcgccga ggtcaaggcg tcgggcctgc gcgggcgcgg cggcgcgggc
     6301 ttccccaccg gtgccaagtg ggaactgtgc cgcaagcagc ccggcgacac caaggtcgtc
     6361 atctgcaacg ccgatgaggg cgatccgggc gcgtacatgg atcgctccat catcgagggc
     6421 aacccgcacc gcgtcatcga agggatgatc attggcgcgt acgccatcgg cgcgcaccag
     6481 ggctacgtgt acatccgcgc cgaatatccg ctggccgtga agacgctgcg cacggcggca
     6541 cggcaagccg aagaggcggg gctgctgggc aaaaacatcc tcggcagtgg cttcgatttt
     6601 tcgctcggca tccggctggg agccggcgcg ttcgtgtgcg gcgaggaaac ggcactgatg
     6661 gcctccatcg aagggcgcgt cggcgagccg cgcccgcgcc cgccctatcc cgccgagtct
     6721 ggactgtggg gctggccgag caacatcaac aatgtcaaga cgtgggcgtc ggttccgatc
     6781 atcatcaacc ggggcgcgga ctggtacgcc ggcatcggca cgccgaaatc caagggcacg
     6841 atgatcttct cgatcgtcgg caagatcaac aacaccggcc tggtcgaggt gccgatgggc
     6901 atttcgctgc gcacgctgat tttcgacatc ggcggcggca tccccaacgg caaggcgttc
     6961 aaggccgcgc agatcggcgg gccgtcgggg ggctgcctgc ccgccgagca tctcgacgtg
     7021 cccatcgact acgagtcgct gacggcgttg ggcgcgatcg tcggctcggg cgggttggtg
     7081 gtggccgacg aagacacgtg catggtggac ttcgcgcgct acttcatggc gttcacgcag
     7141 gaagaatcgt gcggcaagtg cgtcccgtgc cgtgccggca ccaaggcgat gctggccacg
     7201 ctcgagcgca tttgcgcggg cgagggtcag ccgggcgaca tcgagtacct gcaggagatg
     7261 ggtgaacacg tcaagcacac gtcgctgtgc ggcctggggc agaccgcgcc caacccggtg
     7321 ctctccaccc tccgctactt ccgcgatgaa tacgaagcgc acattttcga tcgcacgtgc
     7381 cccgccaaag tgtgcaaggg gctgattacg ttctcggtca tccccgagaa gtgcacaggt
     7441 tgcatggtgt gtctgcgcaa ctgcgcagtg aacgccatta ccggcgcgaa gcaggtcgtg
     7501 cacaccatcg atgccggcct gtgcacacgc tgcggcatgt gcaagagcgt gtgcaagttc
     7561 gatgcgatcc gggtgtcgta gaggcgagca tggttcatct gacgatcaat gatcaacctg
     7621 tcgaagttgc cgaaggtcgc acgctgctgg aagcctgccg cgagcacaac attcacgtgc
     7681 cgacgctgtg ctaccacccg gcgctcgaac cctacggcgc gtgccggctg tgcatggtcg
     7741 aggtgtcgca tccgccgcgc gaaccacggc tggtcgctgc ctgcgcgtat ccgtgcgaag
     7801 aaggcgccgc cgtgaagacc gacaccgaaa cagttcggca cagccgccgc ctcaccgccg
     7861 agctgctggc cggagtagcg cgcacgccgg agatcacgac gctggccgaa tcgttgggtg
     7921 tgcaagaagt ccgctacacc atgcccgatg gagacgcctg cgtgctgtgc ggattgtgcg
     7981 tacgggcctg ccgcgagatc gtgggcgtca gcgccatcag cctgatccgg cgcggcatcg
     8041 ccaagaaagt cagcccgccg ttcgagattg cctcctcgac atgcattggc tgcggcacgt
     8101 gcgtgctgat ctgcccgacc ggcgcactga aacttagcga tgtgacaggc taccgcggcg
     8161 tccacccttc gcactcggag tacagccgcg tctactgcca ggtatgcggc gatgtggact
     8221 tgagcccgcg cttccttgaa gacgtggagt cgctgctggc gggcgcacag gacgaaggat
     8281 gaagacatga gcgaagagtc gtcgtcgcca tcagccgccc gccacccgac gtcagccatt
     8341 cggcttggcg tcgttatctg cgactgcggc aaagccattg ctaggcggct ggacactgaa
     8401 tccctccgtc gacgagcagc cgcgctgccc gacgtggttt acaccgcgcg cgaagcctat
     8461 ccgtgcagca aggatggcca ggctcgcctg cgccaggcga ttcgagatca gggattgaat
     8521 cgcgtgctgg tcgccggctg cacaccgcgc ctcgtcgaga aactgtttcg ggaagcgggt
     8581 cgggcagccg ggctggatga tgcttgcgtc gaggtgaccg acattcgcga gcagtgtgcc
     8641 tacgtccacg acggcgacgc gcagaccatg acggaaaaag cgcacaactt gatcgaaatg
     8701 ggcgtcgagc gcctggccgc aatcagcctg ccacaggtgc acaccgggcg cgtcgtcaaa
     8761 tcggcgatgg tcatggatgg cggactaagc gggttgacgg tggcactgtc gctggccgac
     8821 gaaggcatcg atgtcacgtt ggtcgaaccg ggcggcgcgc tgggcgacct tcagcccccc
     8881 ctgtcgtccc ccccgtcaga cggggggaac agaggggggg ccggggcgcg acggctgatc
     8941 gctgaacgca tcgaggcggt ctcgcgccat cctcgcatcc gcacgctcct gaacgcgcgc
     9001 gtcaccgacg tgaccggccg cccgggcgac tacga
//
LOCUS       DBNHXB010000037         9190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2524716, whole genome shotgun sequence.
ACCESSION   DBNHXB010000037 DBNHXB010000000
VERSION     DBNHXB010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9190
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2524716"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1728
                     /locus_tag="AC162G_01515"
     CDS             97..1728
                     /locus_tag="AC162G_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR5074525.1"
                     /translation="MVVGNQGWQRVTLTMLVALLLMGAGLTIDDISADSSSVVSGVVV
                     NQAGQPLVDAHVRVRATDNLTYTSADGRFMLTGLTPGLGVEIAAWDSGGYYIAGQVIT
                     PPVSGITLTLRPYHTQDNPDYVWLEPTANPSVTLQCGNCHTPILPQWQTNAHGTATSN
                     PRFFSLYNGTDLTGTITLSPGFKLDFPGVAGNCATCHAPGAAANAPFNTDVNTVRGNV
                     TATMHCDFCHKVGGIYLNPTTRVPYANAPGVLSLRLLRPPAGDQIFFGPYDDIKDPDT
                     FLPEMTQSAYCAPCHSFTFWGTPIYQSYNEWLASPYAAKGVECQACHMPPNGDHYFVP
                     PERGGLWHPTEKIPSHLDLGSKDTAFMQNTVAMTLTAQSIVNTVQVSVTLTNVGAGHH
                     VPTDHPGRHMILTIQAMDEAGQPLTQLSGPIVPDWGGAQSGLPGQAFAKVLQDVASGQ
                     APVINYWKQTRIISDSRIPAMGSAASTYTFLAPASGGSITVTAELRFRRAFASLMETK
                     GWDTADVVMETEQVAVAAPTWWGVYLPLVLAEHPR"
     gene            complement(1751..2401)
                     /locus_tag="AC162G_01520"
     CDS             complement(1751..2401)
                     /locus_tag="AC162G_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5074526.1"
                     /translation="MNKIRILLADDHTILREGIRALLEDQPDIVVVGEAEDGRWTVHL
                     AHELRPDVVVMDIAMPLLNGLEATRQIKRDCPDTRILVLTMHDNEEYIRQVLKAGASG
                     YVLKHAAANELVEAIRTVHRGDAALSPTIARKVIEDYLRTDAVRSMPAPDELTPRERE
                     VLQLIAEGHTSKEIAELLGLSIKTVQAHRASLMQKLNLHDRSDLVKYAIQKKIIEV"
     gene            complement(2394..3476)
                     /locus_tag="AC162G_01525"
     CDS             complement(2394..3476)
                     /locus_tag="AC162G_01525"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019350.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR5074527.1"
                     /translation="MAPLRGWTTRVRRRLSRLPIFKRLMLGNSIIIIGGAIAGTLLTH
                     YLSILPGEVFARWILLFASIGIVTSLLVNYWIVKTTLRPLHELGEAVERAQAGRADIP
                     DRVTAEADPDISRLAGALGSMLDRLEGRALQLRALSERAINAQEEERKRIARQLHDET
                     IQSLSTLIINLERLESAVAAGTSNGNLQSRLAAARQLATRSVEDLRKVIYDLRPTMLD
                     DLGLVPAIRWYARSNLEEIGVQVTLDAPDEAMRLPSQLETTLFRIAQEAINNIVRHAE
                     AKSVAISLRRDGGSVCLGIDDDGRGFDVAKITGQALRLRRMGLLGIQERAELVGGEVT
                     LDSVLGRGTRLRVCLPLLAMEEARNE"
     gene            3791..4786
                     /locus_tag="AC162G_01530"
     CDS             3791..4786
                     /locus_tag="AC162G_01530"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR5074528.1"
                     /translation="MCAAPAIPQRHTAEADLLTRAIAGDRDAAGACLDQLGSDNKWLQ
                     HMMLEAMGNCADVRLWQRLLTYLALHRWGEHAHRGHRADSEASKRIDAATVSLFVQDD
                     VPEVAPVKLAVLHEALTEPECRVRNAAATLLGLRGDVRAIEVLVEVVRSGEPDCKLRA
                     VAALGKLNDERAGWPLIEALALDDEQVHHEAGRALGELGDKAVPALIDALKYPQPHVR
                     WHAARALGNIGDARSAAGLLEALTDNDSGVRWAAADALVAIGEPAVPSILEWLSRHTL
                     SEDVRQAVYHALHQMPSRRIQARLQPVLEALRSPASGIEAPGAAYRLLQAWESRS"
     gene            4908..5162
                     /locus_tag="AC162G_01535"
     CDS             4908..5162
                     /locus_tag="AC162G_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074529.1"
                     /translation="MSESRATQAKGEFAMEEPEAALEIMYIEEYLRGRGHTLQSLQSL
                     PAAEARHLLIEASSYASAKLAEVETRAQLMQDLHGTAPPL"
     gene            5178..5522
                     /locus_tag="AC162G_01540"
     CDS             5178..5522
                     /locus_tag="AC162G_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074530.1"
                     /translation="MSRVAEDSSPLLEVIRRKEAEAKRRLAAEREAVEAALAEAERRA
                     RDLLAAAEVEGQRAGEAGRQAVLAEAERNAQAIVAQAQAEAEMLQRAGERQMVAAVAR
                     AFEIVIGGPHET"
     gene            5524..7431
                     /locus_tag="AC162G_01545"
     CDS             5524..7431
                     /locus_tag="AC162G_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013647.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HIR5074531.1"
                     /translation="MTCVQIIGLKTHLMPVVHVLHQLGCVQIDDIAEAGDVSARPLHL
                     DPETVRTQEELGYLVARIEGLLSTLDAGQIESGAPIADQAAGERPISDCVAEARAGLD
                     TLGPSVQSLVSRREALEAEQASLPRYEAVLRKLLPIAPPSARDAANISIGVLVSRGHL
                     GVLDAIAERVLNLTGGRAEIAAQDVDATMRAMLMVIPREFAGEVEALLGREDISRLRL
                     PAGFADQPPDAAVAALSRRIAAIPLELSQIKTELASLAEEWGPRLARWRASLRECLEE
                     FAILSRLGETDLTFVLVGWTPERDVPRVRRALRDEVGPAVVMQTLSLAPEDRRARTPV
                     ALANPGPARPFQSLVRMIALPRYEGIDPTLLMTLCLPLFFGMILGDVGYGALLFLLCL
                     AMLRRFRQPGALRDLVQVVMMGSAWGVVFGFLYGEAFGTLGEKLGLHPIWIDRTSADQ
                     VAGLLLFTVAVGAVHVTLGLVLGVWEAFRERSRHLLLERGGMLVGLIGLFLIVSVAVK
                     WLPAGFMTPGAVVLIVGIGLLGSSLGWLGLLMGPIEFIGLVGNILSYLRIAAIGLASV
                     YLARVANDLAGSVGNVVVGVIIAVLIHALNLALGAFSPTIHSLRLHYVEFFRKFYEGG
                     GRPFEPFGRTT"
     gene            7515..7772
                     /locus_tag="AC162G_01550"
     CDS             7515..7772
                     /locus_tag="AC162G_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR5074532.1"
                     /translation="MTEYLEAHMDATGLVAIGAGLAVGLAAIGTGLAQGRIGSAGVGA
                     VTEKREMLGPVILLIAIPETMVILGFAVAAMVLLLGGGLGG"
     gene            7769..8419
                     /locus_tag="AC162G_01555"
     CDS             7769..8419
                     /locus_tag="AC162G_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014093.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HIR5074533.1"
                     /translation="MSAEAILDAIEASGETEMAHLRADAEARAQRILDEAERKAATIR
                     EQARRAALRPAAGERAQRLHQAKLEALRMVGEVRARLVEEALAETRQRLAGLRAEPGY
                     PLILRRLVEEAIHALGDEKTDGGCPLLEIDPHDDVLLRRILDELGLDLPIVPSLKCCG
                     GVVARSGDGRIVVTNTLEARLERATPFLRRDLAAFFEKEQKEQKEKRELTPVPSLP"
     gene            8438..>9190
                     /locus_tag="AC162G_01560"
     CDS             8438..>9190
                     /locus_tag="AC162G_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HIR5074534.1"
                     /translation="MSDFDYGNARLRAMKSRLLSRRTLDGLAEVGSVQGLITALTNTA
                     YRQTIGAALVRLTGMECLAEALRNDLVATVGQARTFYSGPEGELAALVLRRYDLHNVK
                     TVLRGLAQHVPVNEILANTLPIGELRPADLAELARAANVRPAIDLMATWHLPLAQPLL
                     ELRAAERGRNGAELLEMEVALDRWHYRTAMAAAGKTDSVLSEALALEADAANIMTALR
                     LIGVSDAATVLRERFGSENATPLFVGPGRVLPA"
BASE COUNT         1669 a         2837 c         2967 g         1717 t
ORIGIN      
        1 gaagtcttca tcggcagcgc accccggcag gtgtatctgc cgctgatgtt gaaataatcg
       61 cgccggccgg cccactcggc gcgacacagg aggtgcatgg tggtaggtaa ccagggatgg
      121 caacgcgtga cgctcaccat gctcgtcgcg ctgctgctga tgggcgcggg actgaccatc
      181 gacgacatat ctgccgactc ttcgagtgtg gtgagtggcg tcgtcgtgaa tcaggccggt
      241 caaccgttgg tcgacgcgca tgtccgcgtg cgggccaccg acaacctgac gtacacctct
      301 gctgatgggc gcttcatgct gacgggcctg acgcctgggt tgggtgttga aatagccgca
      361 tgggactccg ggggatacta cattgccggt caggtgatca cgccgccggt gtccggcatc
      421 acgctgacac tgcgccccta tcatactcag gacaacccgg attatgtgtg gctggagcca
      481 acggccaatc ccagcgtgac gcttcaatgc ggcaactgcc acacacctat tctgccacaa
      541 tggcagacga acgcgcatgg cacggcgacc tctaatccac ggttcttttc gctctacaac
      601 ggcacagacc tgaccggcac aatcaccctc tcgcccggct tcaaacttga ctttcccggt
      661 gtggccggta actgcgccac ttgccatgcg ccgggggcgg cagccaacgc gccattcaac
      721 accgacgtga ataccgtgcg cggcaatgtg accgctacca tgcactgtga cttctgccat
      781 aaagtcggcg gcatctactt gaacccgacc acacgcgtcc catatgccaa tgcccccggc
      841 gtgctctcac tgcgcttgct gcgtccgccc gctggcgatc aaatcttctt cggcccgtac
      901 gatgatatca aagacccgga taccttcctg ccagagatga cgcaaagcgc ctattgcgct
      961 ccatgccata gtttcacttt ctggggcacc ccgatttacc agagctacaa tgagtggcta
     1021 gccagtcctt atgcagccaa aggagtagag tgccaggctt gccacatgcc tcccaacggt
     1081 gaccactact tcgtcccgcc cgagcgcggt ggcctttggc atccgaccga gaagattccc
     1141 agccacctcg acctgggtag caaggacacg gctttcatgc agaatacggt tgccatgacg
     1201 ctaacggctc agtcgatcgt caataccgtg caagtcagcg tgacgttgac gaacgtgggg
     1261 gccggtcatc acgtcccgac cgatcatcct ggccggcaca tgatcttgac gattcaggcg
     1321 atggatgaag caggacagcc cttaacccag ttgtcaggcc cgatcgtgcc ggattggggt
     1381 ggcgcacaaa gcgggctgcc tggccaagcc tttgcaaagg tgctgcaaga tgttgccagc
     1441 ggccaggcgc cggtcatcaa ctactggaag cagacgcgca tcatcagcga cagtcgcatc
     1501 ccggcaatgg gcagcgccgc atcgacctac acatttcttg cgccggcatc aggcggatcg
     1561 atcaccgtca cggccgagct gcgcttccgc cgggcgtttg cctcgctgat ggaaactaag
     1621 ggctgggaca ccgccgacgt cgtgatggag actgagcaag tcgctgtcgc cgccccaacg
     1681 tggtggggcg tctatttgcc tctggttcta gctgagcacc cacggtgaaa gtttcggctc
     1741 ataatgcgga tcagacttcg atgatcttct tctgaatcgc gtacttgacc aggtcacttc
     1801 gatcgtgcag gttcagtttt tgcatcaggc tcgcgcgatg ggcctgcacg gtcttgatcg
     1861 acaggccgag cagctcggcg atttctttgc tggtatggcc ttcggcgatc agttgcaata
     1921 cttcacgctc gcgcggcgtc aactcgtccg gcgccggcat ggaccggacg gcgtccgtgc
     1981 gcaaatagtc ttcgatgact ttccgggcga tggttggcga gagcgccgca tcgccgcgat
     2041 gcaccgtgcg gatagcctcc accagttcgt tcgcagcggc atgcttgagc acgtagcccg
     2101 atgcgccggc cttcagcacc tgccggatgt actcctcgtt gtcatgcatg gtgagcacca
     2161 ggatgcgggt gtcgggacaa tcacgcttaa tctggcgcgt ggcctccagg ccgttcagca
     2221 ggggcatggc aatatccatc acgaccacgt ccggccgcag ctcatgcgcc aggtgaacgg
     2281 tccaacgacc atcttccgct tcgccgacca ccacaatgtc cggctggtct tccagcaggg
     2341 cgcgaatgcc ctcgcggagg atggtgtgat cgtccgccag caatatccgg attttattca
     2401 ttgcgagcct cctccatcgc cagcaggggt aggcacaccc gcagccgggt gccgcgaccg
     2461 agcaccgagt cgagcgtcac ttcgcccccg accaattctg cccgctcctg aatgcccaac
     2521 agtcccatgc gtcgcagtcg cagcgcctgc ccggtgatct tggctacatc gaagccgcga
     2581 ccatcgtcgt ctatccccaa gcagacgctg ccgccatccc gcctgaggct gatagcgact
     2641 gacttcgcct cggcgtgacg cacgatgttg ttgatagcct cttgcgcaat gcggaacaga
     2701 gtcgtctcca actgtgacgg cagacgcatg gcctcgtccg gcgcatcgag cgtgacctga
     2761 accccgatct cctccaggtt cgatcgggcg taccagcgga tcgccggcac caacccgaga
     2821 tcgtccagca tcgtcgggcg caggtcataa atgaccttgc gcaggtcttc cacgctgcgg
     2881 gtggccagtt ggcgcgcggc ggccagccga ctctgcaggt tgccattcga cgtcccggcc
     2941 gcgaccgcgc tctccagtcg ctccaggttg atgatgagag tggacagaga ctgaattgtt
     3001 tcgtcgtgca gttgccgtgc tatgcgctta cgctcctcct cttgcgcatt gatggcccgc
     3061 tcggagagtg cgcgcagttg gagtgcgcgc ccctccagcc gatcgagcat cgaaccgagc
     3121 gcgccggcta accggctgat gtcggggtcg gcctctgccg tcacgcgatc aggaatatcg
     3181 gcccgcccgg cctgtgcacg ttcgacggcc tcgcccaatt cgtgcagcgg gcgcagcgtt
     3241 gtcttgacga tccagtagtt gaccaacagg ctggtgacaa tgccgatcga tgcgaacagg
     3301 agaatccacc tggcgaaaac ctcgcccggc aggatagaca ggtagtgcgt gagcagcgtg
     3361 ccggcaatgg cacctccgat aatgataatc gaattcccca gcatcagccg cttgaagatc
     3421 ggcagacgag agaggcgtcg ccgtacacga gtcgtccagc cacggagagg agccattacg
     3481 agcattatgt tataggcgcc cgggggcgaa tagtgccagg attccctgtc caggctgata
     3541 ttcgtcacct cacccggatc gattttaccc caaaataggt tcttcacctg gccgtatctc
     3601 aattcctcag ccgatgcgca gaccagcagg ttactctaca ctgttcacag tacaccgcgt
     3661 ggagggtatg cacacaaaac atgtcaggcc agacgtaggg cggctttcca tagccgccaa
     3721 tttgtggcgc gtatggaaac gcgccctacg gtggatgaca acctttgcgt gcacaccgtg
     3781 gaggataacc atgtgcgctg caccggccat tccacagcgg cacacggcgg aagcggattt
     3841 gctgacacgt gcgatcgccg gcgaccgcga cgctgccggt gcctgcctgg atcagttggg
     3901 ttcagataac aagtggctcc agcacatgat gctggaggcg atgggcaact gcgccgacgt
     3961 gcggttgtgg cagcggcttc tgacctacct ggcgcttcat cgctggggag aacacgctca
     4021 ccgcgggcat cgggcggaca gcgaggcatc caaacggatc gacgcggcga ccgtcagcct
     4081 gtttgttcag gacgatgtgc cggaagtcgc gcccgtgaag ctggccgtgc tgcacgaagc
     4141 gctgaccgag ccggaatgcc gcgtccgaaa cgccgccgcg accttgctcg gcctgcgggg
     4201 cgatgtgcgg gcgatcgagg tgctggtcga agtcgtgcgc agcggcgagc cggactgcaa
     4261 gttgcgggcg gttgccgcgc tcggcaaatt gaacgacgaa cgtgcaggct ggccgctgat
     4321 cgaagcgctg gcgctggacg atgagcaggt gcaccacgaa gccgggcggg cgttgggcga
     4381 actgggggac aaagccgtac ccgccttgat cgatgcgctt aagtatcccc agccgcacgt
     4441 gcgctggcac gcggcccgcg ccttgggcaa tatcggcgat gcccgatcgg cggctggcct
     4501 gctcgaagcg ctgaccgata atgactccgg cgtacgctgg gcagcggctg atgcgctggt
     4561 ggccatcggc gaaccggccg tacccagcat actggaatgg ctatcacgtc acacgctctc
     4621 agaagacgtg cgtcaggctg tctaccacgc gctgcatcag atgccgtcgc gccggatcca
     4681 ggctcgcctg cagcccgtcc tggaggctct gcgcagcccg gcctcaggga tcgaagcgcc
     4741 gggagcagcc taccgcttgc ttcaggcctg ggagagccga tcatagtggt ttccggctaa
     4801 agcctcgact ccggcgtaaa ccgaagtgga atccgctctg gcgcggtttg ccgtggcgat
     4861 gcgcgccggg ctgtttcgaa gacacagtga gaaagggtgg tgaagtaatg agcgaatcac
     4921 gcgcgactca agccaaaggc gaattcgcca tggaagagcc ggaggctgct ctggaaataa
     4981 tgtatatcga agaatacctg cgcggcagag gccatacgct acaaagctta cagagcttgc
     5041 cagcggcaga agccaggcat ctcttgatcg aagcgtcatc gtatgcgtcg gccaagcttg
     5101 ccgaggtgga aacacgcgcg caactcatgc aagaccttca cggtaccgcg ccccctcttt
     5161 agcgggaaga gttcgccatg tcgagagtgg ccgaggattc cagcccgctg ctcgaagtca
     5221 ttcgccgcaa agaggccgag gccaagcgcc ggctcgccgc cgaacgcgaa gcggtcgagg
     5281 ctgccctggc tgaagcggaa cggcgtgctc gcgacctgct ggccgccgcc gaggtcgagg
     5341 gacagcgcgc cggcgaggcg ggacggcagg cagtcctggc cgaggcggag cgcaatgccc
     5401 aggccatcgt cgctcaggca caggccgagg cggaaatgct tcagcgcgcg ggagaacgcc
     5461 agatggtcgc cgccgttgcg cgggcctttg agatcgtgat cggaggtccg catgagactt
     5521 agaatgacgt gtgtgcagat catcggcctt aagacgcatc tcatgccggt ggtgcatgtg
     5581 ctgcaccagt tgggctgcgt gcagatcgac gacatcgccg aggcgggtga tgtttccgcg
     5641 cgccccctgc atctcgatcc cgaaacggtg cggacacagg aagagcttgg ctacctggtg
     5701 gcaaggatcg agggcctgtt gtccacactc gacgccggcc agattgaatc cggcgcaccc
     5761 atcgccgatc aggccgcagg cgaacggccc atctccgact gcgtagccga ggcgcgcgcc
     5821 ggactggaca cactgggtcc ttccgtgcaa tcactcgttt cgcgacgaga ggctttggag
     5881 gcagagcagg catcgctgcc gcgctacgaa gccgtgctac gcaaactgct gccgatcgcg
     5941 ccgccgtctg cgcgcgatgc cgcgaacatt tcgatcggcg tgctggtgag tcgtgggcac
     6001 ctcggcgtgt tggacgccat cgccgagcgc gtgctgaatc tcaccggcgg ccgcgccgag
     6061 atcgcggcac aagacgtgga cgcgacgatg cgggccatgc tcatggtgat cccgcgcgaa
     6121 ttcgccggcg aggtcgaagc gctcctcggc cgcgaggaca tctcgcgcct gcgcctcccc
     6181 gccggatttg ccgatcagcc gccggacgcg gctgtggcgg cgctgagccg gcggatcgcg
     6241 gccataccgc tcgaactgtc gcagatcaag accgagctcg cctcattggc tgaggagtgg
     6301 ggaccacgcc tggcacggtg gcgcgcgtct ctgcgtgagt gcctggaaga gttcgccatc
     6361 ctgtcacgcc ttggcgagac cgacctgacc ttcgtgctcg tcggatggac gcccgagcgc
     6421 gatgtgcccc gggtgcgtcg ggcgctgaga gacgaggtgg gtcctgcggt cgtgatgcag
     6481 accctatcgc tcgcacctga agatcgcaga gcgcgcacgc cggtcgcgct ggcgaacccc
     6541 ggcccggccc ggccgtttca gagcctggtg cgtatgattg ctcttccgcg ttacgagggc
     6601 attgatccca cactgctgat gacgctctgc ctgccgttgt tcttcggtat gattctcggc
     6661 gacgtaggct atggtgcgct cctcttcctg ctgtgtctcg ccatgctgcg gcgattccgc
     6721 cagccgggtg ctctgcgcga tctcgttcag gtagtgatga tgggatcggc gtggggcgtg
     6781 gtcttcgggt ttttgtacgg cgaggcattc ggcacgctcg gcgagaaact gggcctgcac
     6841 ccgatctgga ttgatcgcac gagtgccgat caggtggccg ggctgctgtt gtttaccgtc
     6901 gccgtggggg ccgtccacgt cacactcggt ctcgtcctcg gcgtgtggga ggcattccgg
     6961 gagcgaagca ggcatctgtt gctcgaacgc ggcgggatgc tcgtggggtt gatcgggctt
     7021 ttcctgatcg tgtcggttgc ggtgaagtgg ctgccggccg gattcatgac gcccggggcc
     7081 gtggtgctca tcgtcggcat cggcctgctt ggctcgtcgc tcggctggct ggggttgctc
     7141 atggggccaa tcgagttcat cgggttggtg gggaatattc tctcttatct gcgtattgct
     7201 gccatcgggc tggcctcggt ctatctggcg cgcgtcgcca acgacctggc ggggagcgtc
     7261 ggcaacgttg tggtcggggt catcatcgcc gtgctcattc acgcgctcaa cctggcactc
     7321 ggcgcattca gccctaccat ccacagtttg cgtctgcact atgtggaatt cttccgcaag
     7381 ttctacgagg gtggagggcg accgtttgag ccgttcggac gtacaacgtg aggcgtgaca
     7441 acgtgatgcg taaaacgtga tgcgtgaaac gtaaaacgta aaacgtgaaa cgtaaaacgt
     7501 aagacgttgc acgcatgacg gaatatctgg aggcgcacat ggatgctaca ggattggtcg
     7561 ctatcggcgc gggattggcg gttggcctgg ccgcgatcgg cacgggattg gcccagggcc
     7621 ggatcggatc ggccggcgtc ggcgcggtta ccgagaaacg agaaatgctg gggccggtga
     7681 tcttgctcat cgccattcct gagacgatgg tgatcctcgg gttcgccgtc gccgcaatgg
     7741 tcctgctcct cggtggagga ctgggcggat gagcgcagaa gcgatcctcg acgccatcga
     7801 agcttcgggc gagactgaaa tggctcacct gcgagccgac gccgaagcac gggcgcagag
     7861 aatactggac gaggccgagc gcaaggccgc gacgatacga gaacaagccc ggcgagcagc
     7921 cctgcgaccg gctgccggcg agcgagcgca gcggttgcac caggccaaat tggaagcgct
     7981 gcgcatggta ggcgaggttc gggcgcggct cgtggaggaa gcgctggctg aaacgcgcca
     8041 gcgcctggcc ggtctgcgcg ctgagcctgg ctatccgctc atcttgcgcc ggctggtcga
     8101 ggaagcgatt cacgcgcttg gcgacgagaa gacagacggc ggttgtccat tgttggagat
     8161 cgacccccat gatgacgtgc tgctgcgtcg catcctggac gagctgggtc tcgatctgcc
     8221 gatcgtgccc tctctcaagt gttgcggagg cgtcgtggct cgcagcggcg atggacgcat
     8281 cgtggtgacg aatacactcg aggcgcgcct cgagcgggcc acgccgttcc tgcgtcgaga
     8341 cctggcggcc ttctttgaga aggagcagaa ggaacagaag gaaaagaggg aactcacccc
     8401 agttccttca cttccttaga gttccctgtt ttgactcatg tccgactttg attacggcaa
     8461 cgctcgtttg cgagcgatga aatctcgcct gctttcgcgc agaacgctcg atgggctggc
     8521 cgaagtgggg agcgtgcagg gtctgatcac ggcgctcacc aacacggcct atcgccagac
     8581 gataggagcg gcgctcgtgc gcctgaccgg tatggagtgc ctggccgagg cgctgcggaa
     8641 cgatctggtc gccacggtgg gccaggcacg cacattctat tccgggccgg agggtgagct
     8701 ggcggcactg gtgctgcgcc gctacgacct gcacaacgtc aagactgtgc tgcgcgggtt
     8761 ggcccaacac gtgccggtta atgaaatcct ggcgaatacg ctgccgatcg gcgagctgcg
     8821 gccggcggac ctggccgaac tggcgcgcgc ggcgaatgtc cgcccggcga tcgatttgat
     8881 ggcaacctgg cacctgccgc tggcacagcc gttgctcgag cttcgcgccg ccgaacgagg
     8941 ccggaatggc gcagagctgc tcgaaatgga agtcgcgctt gatcgctggc attaccgcac
     9001 ggcgatggcc gccgcgggca agacagacag cgtcttgagc gaggcactgg cgctggaggc
     9061 cgacgccgcg aacatcatga cggcgctgcg cctcatcggc gtctcagatg cggcgacggt
     9121 tttacgtgag cgattcggat ccgaaaatgc cacacccctg ttcgtcggcc ccggccgcgt
     9181 gctgccggcg
//
LOCUS       DBNHXB010000038         4641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2860478, whole genome shotgun sequence.
ACCESSION   DBNHXB010000038 DBNHXB010000000
VERSION     DBNHXB010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2860478"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            401..1141
                     /gene="rpiB"
                     /locus_tag="AC162G_01565"
     CDS             401..1141
                     /gene="rpiB"
                     /locus_tag="AC162G_01565"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR5074535.1"
                     /translation="MSAQEAHIRDVVRRVLHRTLGLSDRPSGRDAPSASASTLITEQA
                     VAGVERGGRISVPPGALVTPLARQIALERNIALVETPSPRRQAIARPATAPERRTVAI
                     GADHGGFALKETLKAFLEEMGYTSVDCGTHSSDPVDYPDYALAVAELVSSGRAWRGIV
                     VDGAGIGSCMAANKVPGVRAAMCYDQATAVNSREHNDANVLTLGAGLIGATLAKQIAK
                     TWLETDFGGGRHARRVDKIAAIEKRFSK"
     gene            1215..1784
                     /locus_tag="AC162G_01570"
     CDS             1215..1784
                     /locus_tag="AC162G_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR5074536.1"
                     /translation="MAVQVESQTKTHGVTYADYAAMPDDGHRYQVIHGELVMTPAPNE
                     PHQRSLLNLASLIREHARTAALGKVYIAPFDVVLSEDNVVQPDLLFISKERLNIITDA
                     NVQGAPDLVVEFLSPGTAHLDRTQKRDLYARFGVREYWLVSPEAGTIEVLVLRGGKFE
                     RLGLFGAGDSVKSEVLSGLSFKTDVVFEA"
     gene            2052..2744
                     /gene="deoC"
                     /locus_tag="AC162G_01575"
     CDS             2052..2744
                     /gene="deoC"
                     /locus_tag="AC162G_01575"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR5074537.1"
                     /translation="MTSGLGVVPSDAGIAALIDHTLLKPDATEAQIAQLCHEARKYNF
                     AAVCVNPANVKLCAQLLKGSAVRVCTVVGFPLGATPTEVKAYETQQAIDDGATEIDMV
                     INIGALKSKDYVLAARDVATVVATAHAGGAIVKVIIEAALLTDEEKVVASKLCKEAGA
                     DFVKTSTGFASGGATAHDVALIRRAVGPSIGVKAAGGIRTYADAQQMIEAGATRIGAS
                     ASVKIIQEAGAR"
     gene            2780..3073
                     /locus_tag="AC162G_01580"
     CDS             2780..3073
                     /locus_tag="AC162G_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HIR5074538.1"
                     /translation="MNDDDPGTCPACHIGTLQRKSAPYAAWHDDEFVVFSSMAIWICD
                     VCGERIDDESLLDRLFALAGPALSVLDAGGSTGASLPADPSASNDGPRTRRRA"
     gene            3076..3708
                     /gene="ccmP"
                     /locus_tag="AC162G_01585"
     CDS             3076..3708
                     /gene="ccmP"
                     /locus_tag="AC162G_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015176726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031470 - carboxysome [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-carboxysome protein CcmP"
                     /protein_id="HIR5074539.1"
                     /translation="MTDLRSYVFLDSLQPQHAAFLGTVAQGFLPLPGDASLWIEIAPG
                     IEINRITDVALKATRVRPGMQIIERLYGLLEVHSESQAEVRAAGAAILEALDLTEEDR
                     WRPRILSSQVIRNVDPHHTQLINRMRHGHMLLAGHTLYVLEVEPAAYAAIAANEAEKR
                     AEINILEVRAFGSFGRVYLGGEERDIMAGYQAAVDAVEGIRGKEATGRKE"
     gene            3813..4121
                     /locus_tag="AC162G_01590"
     CDS             3813..4121
                     /locus_tag="AC162G_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006599051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HIR5074540.1"
                     /translation="MAEALGMIECKSFAAMVEASDAMVKAAKVELVGYEKTGGGFVTA
                     IVRGDVAAVKAAVEAGVRNAEKVGEVVSVHVIPRPHLNVDLVLPLGREAEAKQEMQGG
                     "
     gene            4121..4411
                     /locus_tag="AC162G_01595"
     CDS             4121..4411
                     /locus_tag="AC162G_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HIR5074541.1"
                     /translation="MQLARVIGTLVATRKDEKMEGMKFLIVRPINPDGQEIASAVVAV
                     DAVGAGVGEVVMIATGSSARQTVLTDRRPCDAVIMAIVDTWEVGGEVKYRKE"
BASE COUNT          869 a         1446 c         1559 g          767 t
ORIGIN      
        1 gcctggatcg tgtattggcc gccgacagac tgggcgatcg ttccccacca tacgtatgcg
       61 gccgccaacg cgccatagcc cccctggcat ctccggccgg cgcgagcgcg tcgatcgccg
      121 cccggcgttc cccaagtctg taggcgcgcc aggccctggg ttatggacca tgttggaaac
      181 gccgcttcag gcgcccactc ggccagccga gtcgtcctcg ccggaggacg cgccgcccgc
      241 gcggcggtcg cgctggctgc gcgaggttag cctggatttt gcacccgctc ccaccgctgc
      301 tcgaaggcgc gccaagtccg gcggctgggg tgcggaattg ccaatccacg ccgagcaatg
      361 cgaaatgtgg gcttaggcgc gggagcgcac gggagggtat gtgtcggcgc aagaagcaca
      421 catccgcgac gtcgtgcggc gggtgctgca tcgcactctg ggcctgagcg atcgcccatc
      481 cggacgcgac gcgccttccg cgagcgcctc gacgctgatc accgagcagg ccgtcgccgg
      541 cgtcgagcgc ggcgggcgga tcagcgtgcc acccggcgcg ctcgtcacgc cgctggcgcg
      601 gcagatcgcc ctcgaacgta acatcgcgct cgtcgaaacc ccctcgccgc ggagacaggc
      661 gattgcccgt ccggcaaccg cgccggagcg ccgcaccgtg gcgatcgggg ccgatcacgg
      721 cggttttgcg ctcaaagaga cactcaaggc ttttcttgaa gaaatgggtt atacttcggt
      781 cgattgcggc acccattcga gcgacccggt cgactaccct gattacgccc tggccgtcgc
      841 cgagctggtg tcgagcggtc gcgcctggcg cggcatcgtc gtcgacgggg ccggcatcgg
      901 gtcgtgtatg gcggcgaaca aggtgccggg cgtgcgcgcc gcaatgtgct atgatcaggc
      961 gactgcggtg aacagccgcg agcacaacga cgcaaacgtg ctgacgctgg gcgccggctt
     1021 gatcggcgcc acgctggcga agcagatcgc caagacctgg ctggagacgg atttcggcgg
     1081 cggccgccac gcccgtcgcg tggacaagat cgccgctatt gaaaagcggt tctcaaaata
     1141 aattgaagga ggcggctctc ggagacccct aaagcagagc ggagggggct gtcgccgaca
     1201 caggacacat cgctatggct gttcaagtag aatctcaaac gaagacgcac ggcgtgacct
     1261 acgccgatta cgcggcaatg ccggacgacg ggcatcgcta tcaggtgatc catggagaac
     1321 tggtcatgac acctgcgccg aatgaacctc atcagcgctc tcttctgaac ctggcctcac
     1381 tgattcgtga gcacgcccgt accgccgcgc tgggcaaggt gtatattgct cctttcgatg
     1441 tcgtactgtc cgaggataat gtcgttcaac ccgatctgct cttcatctct aaggagcggc
     1501 tgaacatcat taccgatgcc aacgtgcaag gcgcgcccga tctggtggtc gaattcctct
     1561 cgcccggtac ggcccatctc gacagaactc agaagcgtga tctgtacgcg cgcttcggcg
     1621 tgcgcgaata ctggctggtg agcccggagg caggcaccat cgaggtactg gtgttgcgcg
     1681 gaggcaagtt cgaacgactg ggcctattcg gcgcgggcga ttccgtgaag tcggaagtgt
     1741 tgagcggtct ctcgtttaag accgacgtcg tgtttgaagc gtaagcggca tctcaaaaat
     1801 tcacgcaacc ctgcaacaga cggcggggca gacggcgcaa agcacgtcaa gaatataagc
     1861 gaatgcaagt cgatacggcc actatcgaga gactcgtcga acagatcacg cgcgaagtgt
     1921 tgctgtcgct gagcgagcag caggccaaag ccgccggctc gttgtctgac gtatgccagt
     1981 gcaaggacgg cgaatgctcg cgcgactgcg ccgatcgcgt tcgcgaagtc gtggcggcgg
     2041 gcgcagtgcg catcacctcc ggcctggggg tggtgccctc cgacgccggt atcgccgccc
     2101 tgatcgatca caccctgctc aaacccgatg cgaccgaggc gcagattgcc cagttgtgcc
     2161 acgaagcgcg caaatacaac ttcgccgctg tgtgcgtcaa tccggcaaac gtcaaattgt
     2221 gcgcgcagtt gctgaagggc agcgccgtgc gcgtgtgcac cgtggtgggg ttcccgctcg
     2281 gcgccacgcc gaccgaggtt aaagcctacg agacgcaaca ggcgatcgac gacggcgcga
     2341 ccgagatcga catggtgatc aatatcggcg cgctgaagag caaggattac gtgctggcgg
     2401 cgcgcgatgt cgcgactgtg gtcgccacgg ctcacgcagg cggtgcgatc gtcaaggtga
     2461 tcatcgaagc ggcgctgctc accgacgaag agaaagtggt cgccagcaag ttgtgcaagg
     2521 aagcgggcgc cgacttcgtg aagacctcga ccggcttcgc gtcaggcggg gcgacggccc
     2581 acgacgtggc gctcattcgg cgcgccgtag gcccgagcat aggcgtgaag gcggcgggag
     2641 gcattcgcac ctacgccgac gcgcagcaga tgatcgaagc cggcgcgacg cgcatcggcg
     2701 ccagcgccag cgtcaagatc atacaggagg ccggcgcgcg ctgaccggcg ctgcttctcc
     2761 gatcaaatca aacatcgcga tgaacgacga cgatcccggg acatgcccgg cctgccatat
     2821 cggcacactc cagcgcaagt cggcgccgta cgcggcctgg cacgacgacg agttcgtcgt
     2881 cttttcgtcg atggcgatct ggatctgcga cgtatgcggt gagcgcatcg acgacgagag
     2941 cctgctcgat cggttgttcg ccctggccgg cccggccctg tccgtgctcg acgcgggcgg
     3001 ctcgaccggc gcctccctgc ccgccgatcc gtccgcatca aacgacggtc cccggacgcg
     3061 taggagagcc tgaccttgac tgatttgaga tcgtacgtct tcctggactc gctgcagccc
     3121 cagcacgccg cctttctagg caccgtcgcg caggggttcc tgccgctgcc cggcgacgcc
     3181 tcgctgtgga tcgaaatcgc gcccggcatc gagatcaatc gcatcaccga cgtggcgctc
     3241 aaggccaccc gcgtgcggcc cggcatgcaa atcatcgagc ggctgtatgg gctgctcgaa
     3301 gtacactccg agtcgcaagc cgaggtgcgc gcggccggcg cggcgatttt ggaggcgctc
     3361 gatctcacgg aagaagaccg ctggaggccg cgcatcctgt ccagccaggt gattcgaaac
     3421 gtcgatccgc atcacacgca gttaatcaac cgcatgcgcc acgggcacat gctgctggcc
     3481 ggtcacaccc tgtacgtcct ggaggtggag ccggcggcct acgcggcgat cgctgcgaac
     3541 gaggccgaga agcgcgccga gatcaacatc ctggaagtgc gcgcctttgg ctcgtttggc
     3601 cgcgtgtacc tgggcggtga agagcgggac atcatggccg gctaccaggc ggcggtcgac
     3661 gcggtcgagg gcatccgggg caaagaggcc accggtcgta aagagtaggg agtagtaatc
     3721 agttatcagt aatcagttat cagtagtcag ttatcagtaa tcagtgcttg tattccactc
     3781 tctactgatc actcacacga ggagggaagc gaatggctga agcattgggc atgatcgagt
     3841 gcaagagctt cgcggcgatg gtcgaggcgt ccgatgcgat ggtcaaggca gccaaggtcg
     3901 agctggtggg ctacgagaag accggcggcg gcttcgtgac ggcgatcgtg cgcggcgacg
     3961 tggcggcagt gaaggccgcc gtcgaagccg gcgtgcgcaa cgccgaaaag gtcggcgaag
     4021 tcgtgtccgt ccacgtcatc ccgcgcccgc acctcaacgt cgatctggtg ctgccgctgg
     4081 gccgcgaagc cgaggccaag caggaaatgc agggcggctg atgcaactcg ctcgtgtgat
     4141 tgggacactc gtcgccacgc gcaaagacga gaaaatggag ggcatgaagt tcctgatcgt
     4201 ccggccgatc aacccggacg gccaggagat cgccagcgcg gtggtcgcgg tggacgccgt
     4261 aggggccggc gtcggagagg tcgtgatgat cgccaccggc tcttcggcgc ggcagacggt
     4321 gctgaccgac cgccggccgt gcgacgccgt catcatggct atcgtcgaca cgtgggaagt
     4381 gggtggcgag gtgaagtacc gcaaggaata gtcgtccctc gtaggggccg gcgcgaagcg
     4441 tgcccctgcc ccaaggaagt gtcgtcgaat gaagcacacg ctcgacatgc caccatcgat
     4501 cgaggaaacg ctcaagcggc gcgaggcgca gttgcgccgg gtgcgcgagg cgtcgctggc
     4561 gatctcggct tcgctcgacc tcgagcgcgt tctggccgag attgtggatc gctgcgcgcg
     4621 gatgctggaa ggcgcgtctg t
//
LOCUS       DBNHXB010000039         6795 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_679819, whole genome shotgun sequence.
ACCESSION   DBNHXB010000039 DBNHXB010000000
VERSION     DBNHXB010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6795)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_679819"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="AC162G_01600"
     CDS             complement(<1..850)
                     /locus_tag="AC162G_01600"
                     /inference="COORDINATES: protein motif:HMM:NF022481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-mannosyl-transferase family"
                     /protein_id="HIR5074542.1"
                     /translation="MTRACPERRRRDEATLVENARARRSSVIPGLAVAAAAGALLGLF
                     LARFWYELDPIGFSALGEWPGTIGLALAASAFLTAQWWFLLRHLLGPNEALPVHLPFA
                     LLLVYVFWPEVDSRLAAILLLASVGLALALVVTGLLTTPSPYPAPFRLRPLAIHRLAP
                     LVLMLVVFAMYALTLGTHVGEADTFEFQVVAPALGIAHPTGYPLYVILGKLFSLLPFG
                     SMAFRVNLTSAVMATLAAVVVYAVTARLGGRRLVAFTAALAFASTVVMWSQSIVAEVY
                     ALNALIA"
     gene            complement(847..2916)
                     /gene="mrdA"
                     /locus_tag="AC162G_01605"
     CDS             complement(847..2916)
                     /gene="mrdA"
                     /locus_tag="AC162G_01605"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR5074543.1"
                     /translation="MQPVSKPQSNPRVAVLYGMVALLMAMFVARLWQMQVIEAGRYET
                     LADSNRFRTIAIDAPRGIIYDRVGALLARNMPAYDVTIVPAQLPDGEENEMAVYRRLS
                     ELLGMPISSRTASLPLRAAGGRGIKDIVDEARGIAPYRPVTVRSNVPRDVALQIAEEN
                     LRLPGVKTVIVSSREYPAGLLASHVVGYTSRISIDLVDRLEESMIEMLSDVSQDSVTA
                     FYRNVQAAKNYNPDTDRVGLTGVEATHEDWLSGTKGERSIEEDVAGREIRTVGTPIDP
                     APGHNLYLTIDRELQLVALEALQQQIDQINTYAGTLRTKRGAVVAMDPRNGQVLALVS
                     LPAYDNNLFARGITFEEYEALLNEPFLPIFNHAIGDQVQPGSVFKIIPAASALQEEII
                     TRRWTWYDSGSIVVPNKYFPNDPRQAQTFYGWYRQGFGNTNIVDALANSVNTFFYVTV
                     GGLDVPGYSKVKGFEDLNSFLDYVRAFGLGRLTGIDLPGEAPGHVPDSQWKRLTYGEN
                     WSLGDTYNLSIGEGFLTATPIQMLNATAAVANGGTLYKPEVVLRITDADGNEVESFRP
                     HILHTLPISPDNLAIVREGMQAAVQWGTAQNAQVAGLNVAGKTGTAEFCDDLAQRLGF
                     CAAGLPKPTHAWFTAYAPADDPQIALVVYIYNGGEGSQVAAPVAQKILQFWHDRQASG
                     GSATNDP"
     gene            complement(2966..3466)
                     /gene="mreD"
                     /locus_tag="AC162G_01610"
     CDS             complement(2966..3466)
                     /gene="mreD"
                     /locus_tag="AC162G_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR5074544.1"
                     /translation="MNPYVAIPFLVVVALVQSTLSPRLQIGAVWPDFMLLVVVSWALL
                     GRLTEALVWSLAGGIVVDLTSGGPFGGTGFALMVATLAAAVTAEGVFRGRAVLPIVTA
                     LVATLAFHGAYLLALLLVGQAVDGLDVLLRVALPATVYNAALSLPVYGLMAAIDRRIK
                     PKPLRW"
     gene            complement(3469..4335)
                     /gene="mreC"
                     /locus_tag="AC162G_01615"
     CDS             complement(3469..4335)
                     /gene="mreC"
                     /locus_tag="AC162G_01615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR5074545.1"
                     /translation="MNRGRSRVWLFPVLSLVTIALLLLHESGFIRPLEDAAQVVIAPL
                     QRALSGFFDGIGDTIGAFRDLRELRERNEELQRLNDALITENVRLKEIEAENATLRDL
                     VNFKQENPTYATQAAAVIGRDPSLYRQFITINAGRREGLEPGMPVVTAGSTLVGRVAE
                     VGLRSSKVQLLADSTSAVNVRIQSSRVTGLAEGQPDAELSMTQIPLDATVNVGDIVLT
                     SGLGGNLPRGLIVGQVTDVSRRDIDLFQSARLRPAADLNRLELVLVITDFEPLPVEPE
                     IEEPTPTPGPTP"
     gene            complement(4372..5454)
                     /gene="mreB"
                     /locus_tag="AC162G_01620"
     CDS             complement(4372..5454)
                     /gene="mreB"
                     /locus_tag="AC162G_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR5074546.1"
                     /translation="MFNPINWLLGLFSLDIGIDLGTANTLVNVRGKGIIINEPSWVAI
                     DKRSKKPLAIGAEAKEMVGRTPGNIIAIRPLRDGVISDFEVTEAMLDYFIKKAHASMF
                     LPVPRPRVVVGIPSGVTEVERRAVYDACVNAGAREAYSIEEPVAAAIGVGLPIMEATG
                     SMIVDIGGGTTEVAVFSLGGIILSRSIRVAGDEMDEDIVQYMRQKYNLLIGERMAERV
                     KISIGSAYPLPEEKVMTVRGRSVVTGLPEAVDVSSIEIREALAVSSQTIVDTLKTTID
                     ETPPEVIADLMETGIALAGGGALLQGIAQRLTEEVKIRVWVAPDPLTAVAVGCGKLLE
                     NVDTLQSVIDSVERSRRKTSRVPISV"
     gene            5589..>6795
                     /locus_tag="AC162G_01625"
     CDS             5589..>6795
                     /locus_tag="AC162G_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5074547.1"
                     /translation="MSVKCLECGFESPEGFSFCGHCGARLSAPPAATAGDDREAAIRR
                     IKEAGDHAKRSDRAAAAFERYQEALALLDSAILASDATLHVQFLNARFDILAERYPLW
                     TAIGQPDRAESDLQDMLGIARRAGDGTRLAKAIIALARQYLDSRQYELARPLLEEEVS
                     LLRTQADRAGEAAALAKLAHLNWRTGRFETVADAFQRAHELRRRAGEPTGLARSYFDL
                     GLLYRDGLSHPFHAVGHFEKAIELARHSGDADLETQGLIGLGMSWTRLGDYAQARTVL
                     EQARQKAGEIQPAEQAAWVIVAQADVLRETEPTDARSTVEQALAPALEVDAPDLRWHA
                     LLENARISQAAGAWSDAYTSLEHMQALERSGQMYAYCAMWSHALLARSYLHTGRRDQA
                     AATSEQAVTL"
BASE COUNT         1294 a         2242 c         2112 g         1147 t
ORIGIN      
        1 ctgcaatcag cgcgttgagc gcatacacct cggcgacgat cgattggctc cacatcacga
       61 ccgtcgaagc gaaggccagc gcggctgtga acgcaaccag gcgccgcccg cccagcctcg
      121 cagttacggc gtacacgacg accgccgcaa gagtcgccat caccgccgat gtcaggttga
      181 cacggaatgc catcgacccg aatggcagca ggctgaagag cttgcccaga atcacgtaca
      241 acgggtatcc ggtgggatgc gcaatgccga gcgccggcgc gacgacctga aactcgaagg
      301 tgtctgcttc gccgacgtgc gtgccgagcg taagcgcgta catcgcgaag acgaccagca
      361 tcaagacgag aggcgcaagc cggtgaatcg ccagcgggcg gaggcgaaag ggcgcggggt
      421 acggcgaagg agtggtgagc aggcctgtca caaccagggc cagcgctaat cccaccgaag
      481 cgaggagcag aatcgcggcc aggcgcgaat cgacctcagg ccagaacacg tataccagga
      541 gcagcgcgaa cggcagatgg acgggaaggg cctcattcgg ccctagcaga tgccgtaaca
      601 gaaaccacca ctgggcggtg aggaaggccg aggcagcaag cgcaagcccg atcgtcccgg
      661 gccactcgcc gagcgcggag aaaccaatgg ggtcaagctc gtaccagaat cgggccagga
      721 acagcccaag cagcgcgccg gccgctgccg cgaccgccag gccggggatc accgacgagc
      781 gacgcgctcg ggcgttttcg acgagagtcg cctcgtccct tcgccttcgc tcagggcagg
      841 cccgcgtcat gggtcattgg tcgcgctgcc gcccgacgcc tggcgatcgt gccagaactg
      901 gagaatcttc tgcgccaccg gcgccgccac ttgcgatccc tcgccgccgt tgtagatata
      961 cacgacgagc gcgatctgtg gatcgtcagc cggcgcataa gcggtaaacc acgcgtgtgt
     1021 gggcttaggc aagcccgccg cgcagaagcc tagccgttgc gccagatcgt cgcagaactc
     1081 ggccgtgccg gtcttaccgg cgacattcag accggcgacc tgcgcgttct gcgccgtacc
     1141 ccactgaacg gcagcctgca taccttcacg cacgatcgcc aggttatccg ggctgatcgg
     1201 aagcgtgtgg aggatgtgcg gccggaagga ttccacctcg ttaccgtcgg cgtcggtgat
     1261 acgcagcacc acttccggtt tgtacagcgt gccgccgttc gctacggctg ctgtcgcatt
     1321 gagcatctgg atcggcgtcg cggttaggaa gccttcaccg atcgacaagt tgtacgtgtc
     1381 gcccagcgac cagttctcgc cgtacgtcag gcgcttccac tgactatctg gcacgtggcc
     1441 cggcgcttcg ccgggcagat cgataccggt tagtcggccc aaaccgaacg cgcgcacgta
     1501 atctaagaaa gaattgagat cttcgaagcc cttgaccttg gaataacccg gcacgtccaa
     1561 cccgccgacg gtcacgtaga agaacgtgtt cacagagttg gccagtgcgt ccacaatatt
     1621 cgtattgcca aagccctgcc gataccagcc gtagaacgtc tgggcctggc gcggatcgtt
     1681 cgggaagtac ttgttgggaa ccacgatcga gccggagtcg taccacgtcc agcgccgtgt
     1741 gataatctcc tcctgcagcg ccgacgccgc cgggatgatc ttaaacactg agccgggctg
     1801 cacctggtcg ccgatggcgt ggttgaatat cggcaagaat ggctcgttca ggagcgcctc
     1861 gtactcctcg aaggtgatgc cgcgcgcgaa aaggttattg tcgtaggccg gaaggctgac
     1921 gagcgccagc acctggccgt tccgtggatc catcgcgaca acggcaccgc gcttggtgcg
     1981 cagtgtaccc gcgtaggtat tgatctggtc gatctgctgc tgcagcgctt ccagcgcgac
     2041 cagttgcagc tcgcggtcga tcgtaagata gaggttgtgt cccggcgccg ggtcgatagg
     2101 cgtcccgacc gtgcgaatct cgcgccctgc gacatcctcc tcgatactgc gctcgccctt
     2161 tgtgccgctc agccaatctt cgtgcgtcgc ctcgacgccc gtcagcccga cgcgatcggt
     2221 atccgggttg tagttcttgg cagcttgcac gttccgataa aacgccgtca ccgaatcctg
     2281 gctcacgtcc gacagcattt cgatcatgct ctcttcgagt cgatcgacca gatcgatcga
     2341 gatacggctc gtgtagccca cgacgtgcga ggcgagcaag ccggcgggat attcgcgaga
     2401 ggagacgatc acggtcttca cgccgggcag cctcaggttt tcctccgcga tctgaagcgc
     2461 cacgtcacgc ggcacgttgc tgcgaacggt caccggacgg tacggcgcga taccccgcgc
     2521 ctcgtcgacg atgtccttga tgccgcgccc gccggcggcc cgcaacggca gcgacgccgt
     2581 ccggctgctg atcggcatgc cgagcaactc gctcagtcgc cgatacacgg ccatctcgtt
     2641 ctcttccccg tcgggcagtt gcgctggcac gatcgtcaca tcgtacgctg gcatgttgcg
     2701 agccagcagg gcgcccacgc gatcgtaaat gatgccgcgc ggcgcgtcga ttgcgatcgt
     2761 ccggaagcgg ttgctgtcgg cgagcgtctc atagcggccg gcctcgatca cctgcatctg
     2821 ccacaggcgc gccacgaaca tcgccatcag cagcgcaacc atgccgtata acacggcgac
     2881 gcgcggattc gattggggct tagagaccgg ctgcatacga aacgactacc cgactacgtg
     2941 accaaacgac tatttgaccg acagactacc accgcagcgg ctttggcttg atgcgccgat
     3001 cgatggccgc cataagcccg tacaccggca ggctcaacgc tgcgttgtac actgtcgcgg
     3061 gaagcgcgac gcgcaggagc acatccaggc cgtctaccgc ctggcctacc agcagcagcg
     3121 ccaatagata cgcgccgtga aacgccagcg tcgctaccag ggccgtcacg atcggtagga
     3181 cggcccggcc gcgaaacacg ccttcggccg tgacagccgc cgccagcgtc gccaccatca
     3241 gggcgaaccc cgtgccgccg aacgggccgc cggacgtcag atcgacgacg atgccgccgg
     3301 ccagggacca gacgagcgcc tcagtcaggc ggccgagcaa cgcccatgac acgacgacca
     3361 ggagcataaa atccggccag acggctccaa tctgcaagcg cggggacaat gtcgattgta
     3421 ccagcgccac gacgacaaga aaagggatgg cgacataagg gttcatggct acggggtcgg
     3481 gccgggcgtg ggcgtcggct cttcgatttc gggctcgacc ggcagtggct cgaaatccgt
     3541 gatcacgagt accagctcga gccggttgag gtcggcagcc ggtcgcaggc gggccgattg
     3601 aaacaggtcg atgtcacggc gagatacatc cgtcacctgg cctacgatca ggccgcgcgg
     3661 cagattgccc cccagaccac tcgtcaagac aatgtcgccc acgttcaccg tcgcgtcgag
     3721 cggaatctgc gtcatgctca gctccgcatc gggttgtccc tcggccaggc ccgttacacg
     3781 cgagctttgg atgcgaacgt tgaccgcgct cgtgctgtcg gccagcaatt gcactttgga
     3841 agagcgcagc cctacctcgg ccacacgacc gaccagcgtc gaccctgctg tgacaaccgg
     3901 catgcctggc tccagccctt cgcggcggcc ggcgttgatg gtgatgaact gccgatacag
     3961 gctgggatcg cggccgatga cggcggcagc ctgtgtcgcg tacgtcgggt tctcttgctt
     4021 gaagttcacc aagtcgcgga gcgtggcatt ctccgcctcg atctctttga ggcgaacgtt
     4081 ctcggtgatc agcgcgtcgt tcaggcgctg caattcctcg tttcgctccc gcagctcgcg
     4141 caggtcgcgg aacgcgccga tcgtgtcacc gattccatcg aagaagcccg acaacgcgcg
     4201 ctgcagcgga gcaatgacga cctgcgccgc atcttccagc ggccggatga agcccgactc
     4261 gtgcagcagg aggagcgcga tcgtcaccag gctgagcacc gggaacagcc atacgcgcga
     4321 acgaccgcgg ttcatataga tttatcccta cccggcgtga ggcgcagcag atcaaacaga
     4381 gatgggcacg cgcgaggtct tgcggcggct gcgctccaca ctgtcgatga ccgactgcag
     4441 ggtgtccaca ttttcgagca gcttgccgca gccgacggcc acggcggtca gtggatccgg
     4501 cgctacccac acccggattt tcacttcctc ggtcaggcgc tgcgcgatac cttgaaggag
     4561 cgcgccgccg cctgccagcg cgatgccggt ctccataagg tcggcgatca cctcaggtgg
     4621 cgtctcgtcg atggtcgtct tcaacgtgtc cacgatggtt tgcgacgaga cagccagagc
     4681 ctcgcggatc tcgatcgaag acacgtcgac ggcctcgggc agaccggtca ccacgctccg
     4741 tccacgcacc gtcatcactt tctcttccgg cagaggatag gccgacccga tgctgatctt
     4801 cacccgttcg gccatacgct cgccgatcag caggttgtac ttctggcgca tgtactggac
     4861 gatgtcttcg tccatctcat cgccggccac acggatgctt cgactgagga tgatgcctcc
     4921 gagcgagaag accgccacct cggtcgtgcc tccgccgatg tcgacgatca tgctgccagt
     4981 cgcctccatg atcggcaggc cgacgccgat ggctgccgcc accggctcct cgatcgagta
     5041 agcctcgcgg gcgcccgcat tgacgcacgc atcgtacacg gcgcgtcgct ctacctccgt
     5101 gacgccggac gggatgccga cgaccacacg cggccggggg acggggagga acatcgacgc
     5161 atgcgccttc ttgatgaagt aatccagcat ggcctcggtc acttcgaaat cgctgatcac
     5221 gccatcgcgc aatgggcgga tggcgatgat gttgcctgga gtgcggccga ccatctcctt
     5281 ggcctccgcg ccgatcgcca gaggcttctt gctgcgcttg tcgatcgcca cccaggacgg
     5341 ctcgttgatg atgatgccct tgccacggac gttgaccagg gtgttcgccg tgcccaggtc
     5401 gatgccaata tccagggaaa atagccccaa cagccagtta atggggttga acaagctgta
     5461 ctccttgcga ggtttgcggc gttcggcgcg gattatacca caagtaccga aagcgcctcc
     5521 gcattcgacc tatccaggaa gccggttttc ggcagcccat accaatgctg atataattgc
     5581 gcaccaatat gtctgtgaaa tgcctcgaat gcggattcga aagccccgag ggattctcgt
     5641 tctgcggcca ctgcggggcc agacttagcg ccccgccagc agccacagcc ggcgacgacc
     5701 gcgaggcggc gatccgccgg atcaaggagg ccggtgacca cgccaagcgc agcgacaggg
     5761 ctgccgccgc gtttgagcgc taccaggagg cgctggccct gctcgactcg gccatcctgg
     5821 cctccgacgc cacactacac gttcagtttc tcaatgcccg tttcgacatt ttggcggagc
     5881 gctacccatt gtggacagcc atcgggcagc ctgatcgggc cgaatctgac ctgcaggaca
     5941 tgctgggaat cgctcgccgc gcaggcgacg ggacacggct ggcgaaggcg atcatcgcgc
     6001 tggcgcgcca atacctagat agccggcaat acgagttggc ccgccccttg ctcgaagaag
     6061 aggtatcctt actgcgaacc caggccgacc gcgccggcga ggcggccgcg ttggccaagc
     6121 tggcacacct gaactggcgc accggtcgat ttgagacggt cgccgacgcg tttcagcgcg
     6181 cgcacgagct gcgccggcgc gccggcgagc ctaccggact ggctcgctcg tacttcgatc
     6241 tcgggctgct ttatcgtgat ggcctgtcgc acccgttcca cgccgtcggc cactttgaga
     6301 aagccatcga gctggcgcgt cactcgggcg acgccgatct agaaacacaa gggctgatcg
     6361 ggctgggcat gagctggaca agactgggcg actacgccca ggcgcgcacc gtgttggagc
     6421 aggcgcgcca gaaggccggc gagatccagc cagccgagca ggcagcctgg gtcatcgtcg
     6481 cgcaggccga cgtgctgcgc gaaacagaac caaccgatgc ccgctcgacg gtcgagcagg
     6541 ctctcgcacc ggcgctcgaa gtcgacgcgc ctgatcttcg atggcatgcg ttgctcgaga
     6601 acgcgcgcat ctcgcaggcc gctggcgcat ggtctgacgc gtacacgtcg ctcgagcata
     6661 tgcaggcgct ggaacgcagc ggccaaatgt acgcctactg cgcgatgtgg tcccatgcgc
     6721 tcttggcgcg cagctatctg cacaccggcc ggcgcgacca ggccgccgcc acgtccgagc
     6781 aggctgtcac gctgc
//
LOCUS       DBNHXB010000040         5932 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3196133, whole genome shotgun sequence.
ACCESSION   DBNHXB010000040 DBNHXB010000000
VERSION     DBNHXB010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3196133"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="AC162G_01630"
     CDS             <1..509
                     /locus_tag="AC162G_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020756101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HIR5074548.1"
                     /translation="QPFDVSADLPELARTPVVVVCAGAKAILDLPLTLEWLETHGVPV
                     IGYQCDEFPAFYSRRSGLPVDARVDTPEEAAAIIVAKWRLEIEGGVLVAVPVPAELEL
                     DTSAGEAAIQAALAEAERNGVRGKAITPFLLERVSRLTGGESLRANVALLENNARVAA
                     RLAKCLAE"
     gene            complement(1036..1887)
                     /locus_tag="AC162G_01635"
     CDS             complement(1036..1887)
                     /locus_tag="AC162G_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR5074549.1"
                     /translation="MIKIGDFSRISRVPVKTLRYYDEVGLLKPVEVDRFTGYRYYSLD
                     QLSRLNRILALKDLGFSLEQIAQLLDENLPAAQIRGMLRLKQAEIQNRVQEEQERLAR
                     VEARLRQIEQEDAMSTNEIVIKKIAPQKVAAIRGVIPTYADQGRLWNELDAYLARRKV
                     NPIGPCLTVYYDTEFKERDVDVQVCEPIGGSLTGEGPVAIAELPGVEMMACALHRGDF
                     HNIGETYDRLIRWIEANGYRIAGPNREVYLRAIASPDTSVVYPAEYLTTDPANRLTEV
                     QFPVEKA"
     gene            complement(2034..2801)
                     /locus_tag="AC162G_01640"
     CDS             complement(2034..2801)
                     /locus_tag="AC162G_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074550.1"
                     /translation="MQTLLTQISTVLLLAGVAFTLFALRKTIRQFRQSRRAAYYILRE
                     EAARSAGRWTIVTIVAVAATIALGVYASRAMPAPAPAPTPTATATQAVPTVGAAPTHT
                     LKPTDTPSLAPTDTPTLTPTATIAPDVPEVLLTPIPNAATPNPAARFEFLTLASRLDG
                     NLNPVDPGLQFPEGASRVYVLFRASGVNNGATWGIFCYEDGAIFDSFVGLWDDGPASQ
                     TSRAFCAHDGRPGAFELRAYLGTMLQFQVQYSLTATP"
     gene            3241..3750
                     /locus_tag="AC162G_01645"
     CDS             3241..3750
                     /locus_tag="AC162G_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR5074551.1"
                     /translation="MIEVAIDSIRVSLMTQQRVVILKDLESDRYLPIWIGPCEAESIT
                     IELQGVAVARPLTHDLLKTVVINLGGKVSQVVVNDLRNDTFYARILLSVNGRQMEIDA
                     RPSDAIALAVRARVPIYVAEAVMEAASIVPEEDMDAERADTDARLSQSPFGDLIDNLD
                     LDDLGPEDK"
     gene            3821..5719
                     /gene="dnaB"
                     /locus_tag="AC162G_01650"
     CDS             3821..5719
                     /gene="dnaB"
                     /locus_tag="AC162G_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR5074552.1"
                     /translation="MATAPIDRMVPHSVEAEEAVLGSLLIDPETIFRVSPFLRPDDFY
                     IVKNRWVYEACLALHERREPIDFVTVTEELEKREQLAEIGGPAFVSHLINVVPTAIHA
                     EGYGRIVERAAVRRKLLQAASDIAQYAYDEAAEIDETIDQSEQALFAVSQRRQSRDLL
                     PISDVIRSYYERIEYLYEHRGEPLGIPTGFVDLDKLLGGLQRSDLIIVAARPGIGKSS
                     LMLSMALSAARKFHQRVAIFTLEMSSEQLVQRMVSAETGIDSQRLRLGELRDDEWPLF
                     VQASSSLSDSLIYLDDTPSISALQLRTKARRLHAEHGLDLILVDYLQLMTGEARAENR
                     VQEISYISRALKGLARELNLPVMAASQLSRSVEQRSDKHPVLSDLRESGCLTGDMLVT
                     LADTGARVPIRSLVGQSGFRVWALNERTLKIEATEVSRAFSTGRKPVYRLETRLGYMI
                     RATGNHKFRMPLGWQRLDKLQVRDWIAVPQPDVILGSESPAYVEYADTGVKSVFVRVH
                     PRPEILQPARQTGALFTTPAITDVYWDEIVSIEPDGEEDVYDLTVPGLSNFVANDIIV
                     HNSIEQDADVVMFIYRDDVYDPQSERKNVAEIIVAKHRNGPTGTIELYFRSHLAQFVN
                     ATRREIAL"
     gene            complement(5830..>5932)
                     /locus_tag="AC162G_01655"
     CDS             complement(5830..>5932)
                     /locus_tag="AC162G_01655"
                     /inference="COORDINATES: protein motif:HMM:NF028226.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HIR5074553.1"
                     /translation="HLLAEEVERQLREAIAGLERVTVHVEPPEGRDD"
BASE COUNT         1115 a         1817 c         1909 g         1091 t
ORIGIN      
        1 gccagccgtt cgacgtgtcg gccgatctgc ccgagctggc gcgcacgccg gtcgtcgtcg
       61 tgtgcgccgg cgcgaaagcg atcctcgatc tgccgctcac gctcgagtgg ctggagacgc
      121 acggcgtacc ggtgatcggc tatcagtgcg acgagttccc ggcgttttac tcgcgtcgca
      181 gcgggctgcc cgtcgacgcg cgcgttgaca cccctgagga agcggcggcc atcatcgtgg
      241 cgaaatggag attagagatt gagggcggcg tgttggtagc cgtgcccgtg cctgcagaac
      301 tggaactcga cacatcagca ggcgaggcgg cgattcaggc ggcgctggct gaagccgagc
      361 gaaacggcgt tcgcggcaag gcgatcacgc cgttcctgct cgaacgggtc agccggttga
      421 ccggcggcga gagcctgcgc gctaacgtcg cgctgctgga gaacaacgcc agggttgcgg
      481 ccaggctagc gaaatgcctg gccgagtagt gactacgtgc gtcgcacgta cccctcttta
      541 ttggctagcc aggatgagcg cgtcgggata ggctgcgaag tactctcgcg cgctgatttg
      601 atcgtagcgc gtaccccagc gggtctcgaa ttccatgatc ccaaaaggcc cttcgccgaa
      661 tagcgagagc acgcgagcgg tcttcgcgat cttatcggtg gcagggtctg agctgcgtcc
      721 accgtgtcca tcgaaaatgg ggacgacgac acctccatcg tactgccggc gagcaaggcc
      781 atcttgtggc gcgctcgctc cgaccggtat gcttcgcgca gcggattgat gtcgcgctcg
      841 gcttccagca agccgggaag cagcttgccg ttaatgcgtg ctgcagcgcg tccctttcca
      901 gagtgatctg gcgctgagcc agtgctgcac cgcacctggt aatgacgtct tctgcctcgg
      961 ccagggcaac gaagtagacg ttcagcaaga agcctcccac aggttcccag cctgcgggag
     1021 ggtgatcttg cttccttacg ccttctcgac tgggaactgc acctcggtga gacgattggc
     1081 agggtcggtc gtcaggtact cggcgggata cacaacgctt gtgtccggcg aggcaatggc
     1141 gcgcaggtac acttcgcggt tggggccagc aatgcggtag ccattggcct cgatccaccg
     1201 gatcagcctg tcgtacgtct cgccgatgtt gtggaagtcg ccgcgatgca gcgcgcaggc
     1261 catcatctca acaccgggca gctcggcaat ggccaccggt ccctcaccgg tcagcgagcc
     1321 gccgatcggc tcgcacacct gcacgtccac gtcgcgctcc ttgaactcgg tgtcgtagta
     1381 gacggtcagg cacggcccga tcggattgac cttgcgccgg gcgagatagg cgtccagttc
     1441 gttccacagg cgtccctgat cggcgtaggt ggggatcaca cctcggatcg cagccacttt
     1501 ctgcggcgcg atcttcttga tcacgatctc gttagtggac atggcatcct cctgttcgat
     1561 ttgcctcagc cgcgcttcga cgcgcgccag tcgctcttgc tcttcttgta cgcgattctg
     1621 aatctcggcc tgcttcaacc gcagcatccc gcgaatctgg gccgcgggca ggttctcgtc
     1681 gagcagttgc gcgatctgct ccagcgagaa gccgaggtcc ttcaaggcga gaatgcggtt
     1741 gagccgggac agttggtcga gcgagtagta gcgataaccg gtgaagcgat cgacctcgac
     1801 gggcttgagc agcccgactt cgtcgtagta acgcagcgtc ttcaccggca cgcggctgat
     1861 tcgagaaaag tctccgatct tgatcatgct gtgcgtggtt ctgcgcgcag aacggtccgg
     1921 ccgaacaact tcgcttacag gatacaccct ccagcgacgg gagagtcaag cggccggtgg
     1981 gggatatttt ctaaggaatc cttccgcagg cacttgacct gcgggaggtt gggtcacggt
     2041 gtggctgtca gcgagtactg cacctggaac tggagcatcg tgcctagata ggcacgcagc
     2101 tcgaacgcgc cggggcggcc gtcgtgcgcg cagaacgccc gcgaggtctg ggaggccggg
     2161 ccgtcatccc atagaccgac gaacgagtcg aagatcgcgc cgtcctcgta gcagaagata
     2221 ccccatgtcg cgccattgtt gacgcctgac gcgcggaaga gcacgtacac gcgcgacgcg
     2281 ccctcaggga attgcaggcc cggatcgacc ggattgaggt ttccgtcgag gcgggaggcc
     2341 agcgtgagga attcgaacct ggccgccggg ttcggcgtgg cggcgttcgg gattggcgtc
     2401 aaaagaactt ccggcacatc cggcgcgatc gtcgcagtcg gcgtgagtgt cggtgtgtcg
     2461 gtcggcgcca ggctgggtgt atcggtcggt ttcagcgtgt gagtgggcgc cgcgccaacc
     2521 gtcggcacgg cttgggtcgc agtcgccgtc ggcgtgggcg cgggcgcggg tgccggcatg
     2581 gcccgcgagg cgtagacacc tagagcgatc gtcgccgcca cggccacgat cgtcacgatc
     2641 gtccagcggc cggcgctgcg ggcggcttct tcgcggagga tgtaatacgc cgcgcggcgc
     2701 gactgcctga actggcggat ggtcttacgc agggcgaaga gcgtgaatgc gacgccggca
     2761 agcaacagga cagtggagat ttgtgtcagc agcgtctgca tgcgttaggc acgttcctcg
     2821 gattggcgcg gcagacaacc cgcaacgggc cgggcagcga ggccgacaga ggcggcgacg
     2881 accagaactc gccgcagcaa gcccccgccc aaccgaagct cactccgttg tcgacacgct
     2941 gcttgtggag tgtccacagt ataccacact cttgtgcaaa aacacgcgat gaggcgtgcg
     3001 cacgcaagga ttatcaggcg agaagccggc atccccagaa cctgcccctt cgcagactca
     3061 gggcaagctc tgagcttgcg aagggtgcgg catctaggac gccttacgcc gtcatcggcc
     3121 ggccaagccg ctgagacgat ttgcgtgcac accgcgaggc tctagagatc acgcctgtgc
     3181 ctcgctgtgg tataatttca gtcgcagggc acgaacgggc tgtgtgagga ggaagtgcgc
     3241 atgatcgaag tcgccatcga cagtattcgg gtcagcttga tgacgcagca gcgcgtcgtc
     3301 atcttgaaag atctcgagag cgatcgatac ctgccgattt ggatcggccc gtgcgaagcg
     3361 gaatcgatca cgatcgagct gcaaggcgtg gccgtggccc ggccgctgac gcatgacttg
     3421 ctcaagaccg tcgtcatcaa cctgggcggc aaggtcagcc aggtcgtcgt caacgacttg
     3481 cgcaacgata ctttctacgc ccgtatcctg ctcagcgtca acggccgcca gatggaaatc
     3541 gacgcgcgac cgagcgacgc catcgcgctg gccgtgcgcg ccagggtgcc gatctatgtc
     3601 gcggaagcgg tgatggaggc cgcgtccatc gtgccggagg aagacatgga tgccgagcgc
     3661 gcggacaccg acgccaggct gtcgcagtcg ccgttcggcg atctgatcga caacctcgat
     3721 ctggacgatc taggccccga agacaaataa cccggctcac ccgattttca gacttcggaa
     3781 gtctcctgaa gacttccgaa gtcttggtcg tggctcagac atggcgactg ctcccatcga
     3841 ccgcatggtt ccgcattccg tcgaagccga ggaagccgtc ctcggctctt tgctcatcga
     3901 ccccgagacg atctttcggg tcagcccctt cctgagaccc gacgacttct atatcgtcaa
     3961 gaaccgctgg gtctacgagg cgtgcctggc gctgcacgag cgccgcgagc cgatcgattt
     4021 cgtgaccgtc acggaggagc tagagaagcg cgagcaactg gccgagatcg gaggcccggc
     4081 tttcgtctcc cacctgatca acgtcgtccc gaccgccatc catgccgagg gctacggccg
     4141 catcgtggaa cgggccgccg ttcggcgcaa gctgctacag gccgccagcg acatcgccca
     4201 gtacgcgtac gacgaagcgg ccgagatcga cgagacgatc gaccaatccg agcaggcgct
     4261 gttcgccgtc agccagcgcc ggcaaagccg cgacctgctt ccgatcagcg acgtgatacg
     4321 ctcgtactac gagcgcatcg aatacctgta cgagcatcgc ggcgagccgc tgggcattcc
     4381 caccgggttc gtcgacctcg acaagctgct gggcggcctg cagcgatccg atctgatcat
     4441 cgtcgcggcg aggcccggca tcggcaagtc cagtttgatg ctgagtatgg cgctcagcgc
     4501 ggcgcgcaag ttccaccagc gtgtggcgat cttcacgctc gaaatgtcga gcgagcaatt
     4561 ggtgcagcgc atggtcagcg ccgagacggg catcgacagc cagcgcctgc ggctgggcga
     4621 gctgcgcgac gacgagtggc cgctgttcgt gcaggcgtct tcgtccctca gcgattcgct
     4681 catctacttg gacgacacgc cgagcatctc cgcgctgcag ctccgcacca aggcgcgccg
     4741 cctgcacgcc gagcacggcc tcgacctgat cctcgtcgat tatctgcaac tgatgacggg
     4801 cgaggcacgc gccgagaacc gcgtgcagga gatctcgtac atctcgcgcg cgctcaaggg
     4861 cctggcgcgc gagctgaacc tgccggtgat ggccgcctcg cagctcagcc gctcggtcga
     4921 gcagcgcagc gacaagcacc ccgtgttgag cgatttacgc gagagcggct gcctgaccgg
     4981 cgatatgctc gtgacgctgg ccgataccgg cgcgcgagtg ccgattcgca gcctcgtcgg
     5041 tcaatcgggc tttagagtct gggcacttaa cgagcggact cttaagatcg aagcaactga
     5101 ggtcagccgc gccttctcga cgggacgaaa gccagtgtat cggctggaga cacgattagg
     5161 ttacatgatt cgcgctacag ggaatcacaa gtttcgaatg cctctgggct ggcaacgcct
     5221 tgataagctg caagtgcgag attggattgc agttccgcag cccgatgtta tcttaggctc
     5281 tgagagtcca gcttacgtag aatacgcaga cactggcgtc aaatccgtgt ttgtccgcgt
     5341 tcatccgcgt cccgaaatct tgcagcccgc gagacaaacg ggagcgctgt tcacaacgcc
     5401 agcaatcacc gatgtctact gggatgagat cgtctcgatt gagcctgatg gagaagaaga
     5461 tgtttacgat ctgaccgttc ccggcctgag caactttgtg gcgaacgaca tcatcgtgca
     5521 caactcgatc gagcaggacg cggatgttgt gatgttcatc taccgcgacg acgtgtacga
     5581 tccgcagagc gagcgcaaga acgtggccga gatcatcgtc gccaagcacc gcaacggccc
     5641 gaccgggacg atcgagctgt acttccgcag ccacctggcg cagttcgtca acgcgacgcg
     5701 gcgggagatt gcgctgtgag tgagagtggg gagttagagt ggggagttag agtggggagt
     5761 tggagttggg ggttagagtg ggtcagatgt acaaactcta actctaactc taactctaac
     5821 tctcatatct caatcgtccc gaccttccgg cggctcgacg tgtaccgtga cccgctccag
     5881 gccggcgatc gcttcgcgca actgccgctc gacttcttcg gcgagtaaat gc
//
LOCUS       DBNHXB010000041         2492 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_176831, whole genome shotgun sequence.
ACCESSION   DBNHXB010000041 DBNHXB010000000
VERSION     DBNHXB010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_176831"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="AC162G_01660"
     CDS             <1..579
                     /locus_tag="AC162G_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074554.1"
                     /translation="MQGRARYITRGGEPIAGFAPDVHDALIASAERLGSDASGALRLF
                     EIDARQALARKIDSLAQARVETPGGRPLPLPIEFEGGLTLLGYDLRAEADRPIDLITY
                     WRARHPAVARQTVFAHMLDRDNRLLTQGDGLNVRLSALEADDVILQHFAIDHPPDATA
                     LAIGLYDPATGRRFTLVADPAIDRVRVTLPGN"
     gene            579..>2492
                     /locus_tag="AC162G_01665"
     CDS             579..>2492
                     /locus_tag="AC162G_01665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR5074555.1"
                     /translation="MTRSTPSASSSNPSGTPAVPAIAAALLLVAFALRTLDIANVPPG
                     LTHDELAQLDVARQVASGDWRLLYPGSYGVEPAYHALLSTAQSIWGTNPLAMHLPSIF
                     GGMLAVVCVYVLARRLFGEGVGLLTLGTIAVVWWPVVLSRVVLREVWTIPLYALALYG
                     FWRGFEAVARHDASRTRRPFVLGGVALGVTQYVHTIPRGLFIVFILFGVYLWIVHRDL
                     FKRAWRDLMVLVIVAEAIAAPLLISAALQPDVDRLPLALYGRPDVGTALIDQLQSSVP
                     WILGQFAFAGDSFWHFNIPYRPIFEPLGAVLFGLGWLAALRRWRQPAHAFALIVFVVS
                     LLPSIFLDPHFPFARLSSVQAIAFAFVGLGGEAIGAFLGRVTSSRIQTVSMAALMGGL
                     FAINAIRTAQDLFGVWSSSPGTRSTYNAELRMLGRYLDTLPEPPPISQCTLWIVYPFD
                     PQYQHSVPQSALPYFVQRRDLEVRWHDCRYSLVIPSSGQFLFAHPDLQPLSDFLGRSL
                     QNPWLENARPIPGVDGALQVDARPALAQKLAEWRRLPVSWPPEVSETTPARLPVNFGH
                     ALELIGYQVQPGDDVRVITYWRVIAPLTEDVLIFTHLYNTPTEIMAQQDQFDAAANSL
                     EPRDMVIQVHDFIH"
BASE COUNT          375 a          787 c          781 g          549 t
ORIGIN      
        1 atgcaggggc gcgcccgcta catcacacgc ggcggcgagc cgattgccgg cttcgcgccg
       61 gacgtccatg acgcgctcat cgccagcgcc gagcgactgg gcagcgacgc cagtggcgcg
      121 ctgcgcctgt tcgagatcga tgcgcgccag gcgcttgctc ggaagatcga ttcgctggcg
      181 caggcccgcg tcgagacgcc ggggggccgg ccgctccctc tgccgatcga gttcgagggt
      241 ggtctgaccc tgctggggta tgatctgcgc gccgaagccg atcgaccgat cgacctgatc
      301 acttactggc gagcacgcca cccggccgtt gcgcgtcaga ccgtattcgc gcacatgctc
      361 gatcgagaca accggctgct cactcagggt gacgggctga acgttcggct cagcgccctc
      421 gaagcggacg atgtgattct tcagcacttc gcgatcgatc acccacccga tgccaccgca
      481 ctggcgatcg ggctgtacga tccggcaacc ggccgccgat tcacgcttgt ggcggacccg
      541 gcgatcgatc gggtgcgcgt gactttgccc ggtaactgat gactcggagc actcccagcg
      601 cctcatcgag caatccctcc ggcacgccgg cggtgccggc gattgcggct gcgctcctgc
      661 tcgtggcgtt tgctctgcgc acactcgaca tcgccaatgt cccgcccggc ctgacgcacg
      721 atgagcttgc gcagctcgac gtggcacggc aggtggcaag cggcgattgg cgtttgttgt
      781 atccgggtag ctacggggtg gaaccggcct accatgcgct cttgtccact gcgcagtcta
      841 tctggggaac aaacccgctg gccatgcatt tgccgtcaat tttcggcgga atgctcgccg
      901 tggtctgtgt ttacgtgctg gcccggaggc tttttggcga aggcgtggga ctgctcacac
      961 tgggcaccat cgccgtcgtc tggtggccgg tggtgttaag ccgggtcgtt ttgcgcgagg
     1021 tgtggacgat accgctgtac gcgctggcgc tgtacggatt ctggcgtggc ttcgaagcgg
     1081 tggcgcgcca cgacgcttct cgaacacgac gcccatttgt cctggggggt gtcgcgcttg
     1141 gcgtcacaca atatgttcac accatcccgc gtgggctgtt tatcgtcttt atcctgtttg
     1201 gagtgtatct gtggatcgtt catcgagacc tgttcaagcg cgcctggcgc gatctcatgg
     1261 tgttggtgat cgtggccgaa gccatcgccg cgccgctgct catcagcgcc gccttgcagc
     1321 ccgacgtcga tcggctgccg ctggcactct acggacggcc cgatgtgggc acggcgctca
     1381 tcgatcaact ccaatcgtct gtcccttgga tactcggcca atttgcgttt gcgggcgaca
     1441 gtttttggca ctttaacatc ccctaccgcc cgatcttcga gccgctgggc gctgtcctgt
     1501 ttgggctggg atggctggcc gcgctccggc gttggcgtca accggcgcac gcctttgccc
     1561 tcatcgtttt cgtcgtctcg ctcttgccca gcatctttct cgatccgcat tttccatttg
     1621 cgcgcctgag cagcgtgcag gcgattgcgt tcgcattcgt cgggcttggg ggcgaggcga
     1681 tcggcgcatt cctggggcgt gtcacgtcga gccgaattca aaccgtatcg atggcggcgc
     1741 tgatgggcgg gctgtttgcg atcaatgcca ttcgcaccgc gcaggacttg ttcggcgtgt
     1801 ggtccagctc gcccggcacg cgctcgacgt acaatgccga actgcgcatg ctggggcgtt
     1861 acctggatac gctgccggag ccgcctccga tttcccagtg tacattgtgg atcgtctacc
     1921 cttttgatcc tcagtatcaa cattcggttc cccagtctgc gctgccgtac ttcgtgcagc
     1981 gccgtgatct ggaggtacgc tggcacgatt gccggtacag cctggtcatc ccatcttcgg
     2041 gacagttcct gttcgctcat cccgatcttc agcccttgtc tgattttctc ggtcggtcgc
     2101 tccagaaccc gtggctggaa aacgcgcggc cgattcccgg tgtggacgga gcgctgcagg
     2161 tggatgcgcg gccggcgctg gcgcagaagc tggcggagtg gcgccgattg ccagtgagtt
     2221 ggccgcctga agtgagcgaa accacgccgg cccgactacc ggtcaatttc ggtcacgctc
     2281 tcgagttgat cggctatcag gttcagccgg gtgatgacgt tcgggtcatc acgtactggc
     2341 gcgtcattgc cccgttgacg gaagacgtgt tgatcttcac acatctttat aacacaccga
     2401 ccgaaatcat ggcccagcag gatcagtttg atgcggcggc aaactctctc gagccgcgcg
     2461 acatggttat ccaggtacac gactttatcc at
//
LOCUS       DBNHXB010000042         5322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2525150, whole genome shotgun sequence.
ACCESSION   DBNHXB010000042 DBNHXB010000000
VERSION     DBNHXB010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2525150"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..1654)
                     /locus_tag="AC162G_01670"
     CDS             complement(245..1654)
                     /locus_tag="AC162G_01670"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR5074556.1"
                     /translation="MKAMDALLQSLQNSVAPSDGRVAVADEARLKTRMDGLVYEAAFG
                     DGETRAAARWLIWEIAQALGIRPASIHELYMARGRGAIKRSFTVPAMNLRMLAYDSAR
                     AVFRAACAIDAGAIIFEIARSEIGYTDQRPAEYASAVLAAAIKEGHRGPLFIQGDHFQ
                     INAKKFAETPDAEMRAVCDLSSEAIAAGFYNIDIDTSTLVDLSQPTTYEQQRANFERC
                     AEVSRFVRERQPAGVTISLGGEIGEVGGKNSNEEELRAFMDGYNAALGGMTGISKISV
                     QTGTSHGGVVLPDGTLADVAIDFDVLRRLSDVARAEYHLGGAVQHGASTLPESAFGKF
                     VECGAVEVHLATAFQTLIMDHETIPRQLRDEIRDWLYHNAMNERKAKDTDEQFIYKTR
                     KKAIGPFKRDFWNLSAEARAAIGQALEARFAFLFEQLGVRDTAHIVAEHVHPVEIHQP
                     MPAGAAKAVKAESVEGLAD"
     gene            complement(1920..2120)
                     /locus_tag="AC162G_01675"
     CDS             complement(1920..2120)
                     /locus_tag="AC162G_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HIR5074557.1"
                     /translation="MKVRIDREDDALYFELDDAEIVESEEVQPGIILDFDNDGRLVGI
                     EILDLSTRLTPEKLRLMQFETV"
     gene            complement(2117..2365)
                     /locus_tag="AC162G_01680"
     CDS             complement(2117..2365)
                     /locus_tag="AC162G_01680"
                     /inference="COORDINATES: protein motif:HMM:NF025442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HIR5074558.1"
                     /translation="MQEIEFSKHAKYMLLRRKIPEEWVWRVINTPNKKRRGDDDNMHY
                     TKAIREKEGRVLHVVVNTHVHPHRIVTLFFDRRLGQKK"
     gene            complement(2682..2981)
                     /locus_tag="AC162G_01685"
     CDS             complement(2682..2981)
                     /locus_tag="AC162G_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074559.1"
                     /translation="MASELEKSLKSAAEKIAQYIDDAAMLTVETRAIEIGAEGSPQFD
                     QARAVARSIIRMDGDSETVVPVRPSGKPGQTEVDAALTSCTSATSPRRSSIARTS"
     gene            complement(2971..3780)
                     /locus_tag="AC162G_01690"
     CDS             complement(2971..3780)
                     /locus_tag="AC162G_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074560.1"
                     /translation="MSDHQASPPSFEFQASVALAGVLDFWSAAPPLAEAVSFEAGSAV
                     EDMPVWRVDLPGDLGAASAALATGESQVEAAQRALDAAVERLQTFVSAQAAAPSFDAS
                     SPAAALGAPEAEALALLNEIQTGAPAVSFGLGEKIVAGLDTVTRQFQEFVDALLRNVG
                     SYAWVETRIEGRLVCRTSIGWSGDAKHLWLGPVEAQVRALHQRALSIAIESRNTFVRT
                     FIVSAQSAAKLVTLLSVPGGAILALPTAWRYISQVLAEIKAHSYEETSHGK"
     gene            complement(3854..>5322)
                     /locus_tag="AC162G_01695"
     CDS             complement(3854..>5322)
                     /locus_tag="AC162G_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015113683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074561.1"
                     /translation="PYRFQYQLDVQYAVGRIHFDSLEEYAQYARSVVDAEKGKIALPR
                     QAAFFGVQNPDDKATNLSAVELVKPLADELAQDQKNWTFRTLLKDEATKANLSALLGG
                     AETPALLFSASHGMGFPSGDERQLPHQGALLCRDWPGPNAWRKPIPEDHYFSAGDVGE
                     DARLVGLIAFLFACYGGGTPRFDEFIRKDGKRREIAPHSFMARLPQRLLGHPKGGALA
                     VAGHVDRAWGYSFMWERAGRQTEVFKSTLKRLMEGHPLGSAIEYFNERYAELSSDLSV
                     ELEEIEFGATPDDLKLAGMWTANNDARSYVVLGDPAVRLPVADKPAAERPEIGHVTIQ
                     STAAPREPAPAEVLAATSSGTVAAVDYGLIETIGKAKAGLTDSLQKFADKLGETLQKA
                     VDDISSLEVLTYTADDLNDVKYEAGRFSGSAQLRAMSRFTIDGDALVCIPSRQGEVDE
                     ALWAIHMDMTRQAQVNRVEMLKTAVSAAAGLLGALKAL"
BASE COUNT          917 a         1710 c         1625 g         1070 t
ORIGIN      
        1 ttgtctggcc tcgccgcccg cgatcggcag gctgtagagc tgccagcgcc cggtcttgtt
       61 ccacatgaag acgagggact tgccgccggg agacaggtga aatcccccgc cccaggcgaa
      121 aacgttgggg atgccgaaca gcgtatcgag atcgggaagg gtaggggaca cgggcctctc
      181 caaaggtgat acgtgatcac gtgatcacgt gatcacatat cacctatcac gcaccacgta
      241 ttactcagtc cgccaaccct tcgacggatt ccgccttgac tgctttcgcc gcaccggccg
      301 gcatcggctg gtgaatctcg accggatgaa cgtgctcggc gacgatgtgc gccgtgtcgc
      361 gcacgccgag ctgctcgaac aggaaggcga accgcgcttc gagcgcctgg ccgatcgccg
      421 cgcgcgcctc ggcagacagg ttccagaaat cgcgcttgaa cgggccgatg gctttcttgc
      481 gcgtcttgta gatgaactgc tcgtcggtgt ctttggcctt gcgctcgttc atcgcgttgt
      541 gatacagcca gtcgcggatc tcgtcgcgca gttgcctggg gatcgtctcg tggtccatga
      601 tcagcgtctg gaaagcggtc gccagatgca cctcgaccgc accacactcg acgaatttgc
      661 cgaaggcgga ttcgggcagc gtcgacgcgc cgtgctgcac cgcgccgccc aggtgatact
      721 cggcgcgcgc cacgtcgctc agccgtcgca gcacatcgaa gtcgatcgcc acatcggcga
      781 gtgtgccgtc gggcagcacc acgccgccgt gcgacgtgcc ggtctggacg ctgatcttgc
      841 tgatgccggt catgccgccg agcgcggcgt tgtatccgtc catgaaggcg cgcagctctt
      901 cttcgttcga gttcttgccg ccaacctcgc cgatctcgcc gccgagcgag atcgtcacgc
      961 cggcgggctg ccgctcccgc acgaagcggc tcacctctgc acaacgctca aagttggcgc
     1021 gctgctgctc atacgtcgtc ggctgcgaca ggtcgacgag cgtcgaggta tcaatgtcga
     1081 tgttgtagaa gccggcggcg atggcttcgc tcgacaggtc gcataccgcc cgcatttcgg
     1141 catccggtgt ttcggcgaac ttcttagcgt tgatctgaaa gtgatcgccc tggatgaata
     1201 gcgggccgcg atgtccctct ttgatggcgg cggccagcac agcgctggcg tactcggccg
     1261 ggcgctgatc ggtgtagcca atctcggagc gggcgatctc gaagatgatc gcgccggcgt
     1321 cgatggcgca cgcggcgcgg aacacggcgc gcgccgagtc gtaagccagc atgcgcagat
     1381 tcatcgcggg cacggtgaag ctgcgcttga tcgcgccccg gccgcgggcc atatacagct
     1441 cgtgaatcga ggccgggcgg atgccgagcg cctgcgcgat ctcccagatc agccagcgcg
     1501 cggcagccct cgtctcgccg tcgccgaacg cagcctcgta gacgagaccg tccatacgcg
     1561 tcttgagcct ggcttcgtcc gcgacggcca cgcgcccgtc gctcggcgcg acggagttct
     1621 gcagcgattg cagcagtgca tccattgcct tcataggtca tcttctccgg gttgatgcga
     1681 tgtgtgatgg tgcttcaagc cagcggaaca gcgcccgccg gatggcgcga ttccagcgtg
     1741 cgtcgcgcgg aaacttctcg cgcgtggtga cgatcagtct aacgagatcc gatgcatagg
     1801 ggcgggcatc ggcaggcttc cgtgtgtgtg ccatggggcg acacttgcct tgactgccct
     1861 gatctctgct gttggcgacc tgggagagtc agaacggata cccgcttttg ccactgcctt
     1921 tacaccgtct cgaattgcat gagacgtaac ttctctggcg tcaggcgggt gctcaaatcg
     1981 agaatctcaa tcccaactaa ccggccatca ttgtcaaaat ccaggatgat gccgggttgg
     2041 acttcttcgg actcgactat ctccgcgtca tccaactcaa agtacagcgc gtcgtcttcc
     2101 ctgtcaattc tgactttcat ttcttctgtc ccaaacgacg gtcaaaaaac agagtcacaa
     2161 tccggtgagg gtgaacatgg gtgttgacca cgacatgcaa tacccgcccc tccttttcgc
     2221 ggatggcttt ggtgtagtgc atattgtcat cgtcgccccg ccgtttcttg ttgggtgtgt
     2281 tgatgactcg ccaaacccac tcttcgggga tttttcgtct caggagcatg tacttcgcgt
     2341 gcttcgagaa ctcaatctcc tgcatgggta cgttcccagt tcacatgaac cggctagagt
     2401 gtaccacaac ttttcacccg ctcctattgt gggccttctg gaaccatcgc gtccaatgac
     2461 ctgctcagcc tgtcgtgcca tgcttggagt tgacgtaccc ttacatcgcg atagcacagc
     2521 cagtcgccag actgcgccac tatcgcatca accgatccgg catccgtcgg cgcgcagagc
     2581 gtgttccgcg gcaaatgagt ctccgagccg ttgcgaaatt cgacaggtcg ggagagatgt
     2641 tgcttaggtg ccgctcgtct tcaaggcgtc gagcagcgcg ctcaggatgt gcgcgcgata
     2701 ctcgatcgcc gtggcgacgt tgcgctggtg cagctcgtaa gcgccgcgtc gacctccgtc
     2761 tggcccggct tgcccgaagg ccgcaccggc acgacagtct cactgtcgcc gtccatgcgg
     2821 ataatgctgc gcgcgacagc ccgggcctga tcgaactggg ggctgccctc cgcgccgatt
     2881 tcgatggcgc gcgtctcgac cgtcagcatg gcggcgtcgt cgatgtactg cgcgatcttc
     2941 tcggccgcgc tcttcagtga tttctccagt tcacttgcca tgcgatgtct cctcgtatga
     3001 atgcgccttg atttcagcca gcacctggct gatgtaccgc cacgccgtgg gtagtgccag
     3061 gatggcgccg ccaggcacgc tcagcagcgt cacaagcttc gccgcgcttt gcgcggagac
     3121 aatgaaagtc ctgacgaagg tgttgcgcga ttcgatcgcg atgctcagcg cgcgctgatg
     3181 cagtgcgcgc acctgcgcct cgacgggacc gagccacagg tgtttcgcgt cgccactcca
     3241 gccgatcgac gttcggcaca ccaatcgacc ctcgatccgg gtttcgaccc acgcgtagct
     3301 gccgacgttt cgaagcagcg catcgacgaa ctcctggaac tgccgggtta cggtgtccag
     3361 gccggcgacg atcttctcgc ccagaccgaa gctgacggcc ggcgcgccgg tctgaatctc
     3421 gttcagcagt gcgagagctt ctgcttccgg agcgcccagc gcggccgcgg gcgacgaggc
     3481 gtcgaacgac ggcgcggccg cctgtgcgct gacaaacgtt tgcagccgct cgacagcggc
     3541 gtccagcgcg cgctgagcgg cctcgacctg agattctccg gttgccagcg ccgctgaagc
     3601 ggcgccgaga tcgcctggca ggtcgacgcg ccagaccggc atatcctcga ccgccgatcc
     3661 ggcttcgaac gagaccgcct ccgccaaagg aggtgcggca gaccagaagt ccagcacgcc
     3721 ggcgagcgcg acagacgcct gaaactcaaa tgagggtggt gatgcctgat gatcgctcac
     3781 gtgcaatacc gtagggcagg tttcttacct gccctcaaca atggcggcta tggaaagccg
     3841 ctttacattg tgtttagagc gcctttaacg ctcccagcag ccctgcagcc gccgacacgg
     3901 ctgtcttgag catttctacc cggttcacct gcgcctgcct cgtcatgtcc atgtgaatcg
     3961 cccacagcgc ctcgtccact tctccctgcc gggaggggat gcacaccagc gcatcgccgt
     4021 cgatcgtgaa gcgcgacatg gcgcgcagtt gagccgaccc actgaaccgg cccgcttcat
     4081 acttcacgtc gttcaaatcg tctgccgtgt acgtcagcac ttccaggctg gagatgtcgt
     4141 cgaccgcttt ctggagggtc tcgccgagct tgtcggcgaa tttctgcagc gagtcggtca
     4201 ggccggcttt agctttgccg atcgtttcta tcaagccgta atccaccgcc gccaccgtcc
     4261 cactgcttgt ggcggccagg acctcggctg gcgccggctc acggggagcc gcagtggatt
     4321 gaattgttac atggccaatt tccggccgtt cggcggcagg cttgtctgcg acaggcagtc
     4381 gtaccgccgg atcgccgagt acgacgtagc tgcgcgcgtc attgttcgcc gtccacatgc
     4441 cggccagctt cagatcgtcc ggtgtcgcgc cgaactcgat ctcttcaagc tcgacgctca
     4501 agtcggaaga taattcggcg tatcgctcgt tgaagtattc gatcgcggag ccgagagggt
     4561 ggccctccat cagacgtttc aaagtgctct tgaagacttc tgtctggcgg cctgcgcgct
     4621 cccacatgaa tgagtagccc caggcgcgat cgacgtggcc ggcgaccgcc agcgcgccgc
     4681 ccttgggatg gccgagaagc cgctgcggca ggcgcgccat aaacgagtgc ggcgcaattt
     4741 cccggcgttt gccgtccttg cgaatgaact cgtcgaagcg gggcgtgccg ccgccgtagc
     4801 aggcgaacag gaacgcgatc agacctacca ggcgcgcatc ctcgccaacg tcgccggcgg
     4861 agaagtagtg atcttcaggg atcggcttgc gccaggcatt cggcccgggc cagtcccggc
     4921 acagcaaagc gccttggtga ggcaactggc gctcgtcgcc gctggggaag cccatcccgt
     4981 ggctcgccga gaacagcagg gcaggtgttt ccgccccacc gagcagggcg ctcaggtttg
     5041 ccttggtcgc ttcgtctttc agcagtgtgc ggaacgtcca gttcttctga tcctgcgcta
     5101 actcatcggc aagcggcttg accagttcga ctgcgctgag gttcgtcgct ttgtcgtcgg
     5161 gattctgcac gccgaagaac gccgcctgtc tcggcagcgc gatcttgccc ttttcggcgt
     5221 cgacgacgct gcgcgcatac tgtgcgtatt cttccagcga gtcgaagtga atgcggccga
     5281 cggcatactg cacgtcgagc tggtactgaa agcgataggg ga
//
LOCUS       DBNHXB010000043         6273 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3532034, whole genome shotgun sequence.
ACCESSION   DBNHXB010000043 DBNHXB010000000
VERSION     DBNHXB010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3532034"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2698
                     /locus_tag="AC162G_01700"
     CDS             <1..2698
                     /locus_tag="AC162G_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013124.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5074562.1"
                     /translation="STLKELADERVAAREAITRLRQAPVMFELGARPHPPRELYRAYV
                     DQSHIFVGIYWQKYGWVAPGETLSGLEDEYRLSGDRPKLIYIKSPAPDQEPHLAKLLD
                     RIRGDDRASYKSFSTCAELCELIENDLAMVLTERFEQVQRGEALPAAPVTRASHVPRP
                     PTPLVGRERELAAVKLMLARSDVGLLTLTGPGGTGKTRLAIQAALDLIDSFKHGVSFV
                     ELAPIGDPKLVPTAIAQELDIREARDGRSILENLKDYLRDKHVLLVLDNFEQVVSAGP
                     VVVELLGVSPRLKVLVTSRTLLRVRGEQEMPVRPLEVPERRRSLDAERLSQYASVELF
                     IQRAQAAKPDFEITNESAPAVAEICYRLDGLPLAIELAAARIRILTPQALLARLERRL
                     DVLRGGPRDLPARQQTLRSTIDWSHNLLDNHAKVLFRRLSVFVGGWTLEAAEAVCNAT
                     GDLDVDTLDEMESLIDNSLLKPTEEAAGEMRFGMLETIREYARERLSESGEEDSLRER
                     HAEYHLALAEAIQPRLLTSERRRWLDRLEIEHDNMRAALDCCRLGRCDASVGLRLSAA
                     LAWFWQTRAYLHEGRKHLEAVLARPDAAARTAARANALYGAGVLAWSLGDYAVARACG
                     EESAAIARDVGDQRQAAYALTQLGLVIASQGDTETASALYAEAVKLFREVKDAWGEAF
                     ALTWLAEATLVSDHPLMAQPLYEEGLALWRRVGDPWGTGIALLVLGALALVQSDAAKA
                     YALVEEGMTLLREAGDRWTLSFGIAGLGDVLLWQGDYAQAGAQFAEGLRLSRESGNPL
                     TMVMSLVGLAGVTAGRSLRESPEQRDENLRRTATLVGAADALREALGLHLFRVLRLTY
                     EPRVALIRAQVDEAIWGPAWAEGHAMSLDRAIAYALEKEPHA"
     gene            2691..5447
                     /locus_tag="AC162G_01705"
     CDS             2691..5447
                     /locus_tag="AC162G_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5074563.1"
                     /translation="MPESKSVIRTPDQRLRVFVSSTLQELAEERVAARQAITHLRLAP
                     VLFELGARPHPPRELYRAYLDQSHIFVGVYWQKYGWIAPSETISGLEDEYRLSGERPK
                     LIYVKTPAPDREPCLKALLDRIRSDDTASYKPFTTAAELRDLIENDLAMILTERFESA
                     HLAAGAPSEEATRAQGNLPVVRTPLVGRARELALTRELLLRESSPARLVTLTGPGGIG
                     KTRLALEAANSLGEWFSDGIYLISLAPLTDPSLIVPAIAQTLGMRETADRPLRDNLID
                     YLHDKRILLLLDNFEHVVAGAGAVGELLEACPLLKIVATSQTPLRIRGERELPLPPLA
                     LPRIGALAARSGDIVSQLSQYAAVELFIQRARDVRPDLSVTNETAPAVAEICYRLDGL
                     PLAIELAAARIKVLSPQALLARLERRLDVLKRGARDLPARQQTMRGALDWSHDLLCES
                     ERTLFRRVSVFVGGCTLEAVEFVCVGLGTDEVDLLDQLTSLVDKSLLVQDEMPGGEPR
                     FRMLQMIQGYARQKLVESGEANVFLRRHAGFYCMMAEEAEPYLMGAARTAWLDRLEME
                     LDNLRAALDWSRQSGADMLLRLSGSLAWFWFMRGHLSEGRAWLDSAIACAPRLAATAD
                     AARARALWAAGGLAWAQGDYARARLLIQESLAIYREVHDRHGSAHALSFLGLVMLSLA
                     EPSEAHRLCEEAVSLSREVNDKWGEAFALRCMGDAALLLGDVVGARALYETSLGLWRK
                     VNDPWGISMPLNDLGRVAYVREDYAEARAAYEESVALLRRVGDKWGLALVLTNLANVL
                     IHQDVQAARTVLQECLSTWRELGNKTGVAQCLAAYAGLAAVERRPALAAQLFGAADAQ
                     LGAIGYRPEGMFRAQIERNRSVGRAQLDETTWRAEWERGRGLPTDEAIALAMQLEQLP
                     TQIE"
     gene            complement(5594..>6273)
                     /locus_tag="AC162G_01710"
     CDS             complement(5594..>6273)
                     /locus_tag="AC162G_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074564.1"
                     /translation="YLPAIHKSGCAPIPGVSYGAVAPDPVIGIDPQNDYRINLALRGY
                     TPVDELKAIFDIGTGSDPNAPQFRYLFADVRTPAISTVYALYRWDTTCNCPSTQIVTK
                     PAVSVAGLATAPGEIIHLPDSGYEISPGYDAMVLYASQTRLVAKYTREDDIVAGYTLY
                     LENICLEPSLLALYQALNAAGRQQLPALAGSQPVGRAIGTEIGISIRDAGRLQDPRAR
                     NAWWLYP"
BASE COUNT         1067 a         1911 c         2187 g         1108 t
ORIGIN      
        1 ttcgacgctg aaggagctgg ccgacgagcg cgtggccgcg cgcgaggcca tcacccgact
       61 gcgccaggcg ccggtgatgt tcgagctggg cgcgcgcccg catccgccgc gcgagctgta
      121 tcgagcctac gtcgaccaga gccacatctt cgtcggcatc tactggcaga agtatggctg
      181 ggtggcgccc ggcgagacac tctcgggcct ggaagatgag taccgcctgt caggcgatcg
      241 acccaagctg atctatatca agtcgccggc gcccgatcag gagccgcatc tggccaaatt
      301 gctagaccga atccggggtg atgaccgggc ctcgtacaag tcattctcaa catgcgccga
      361 gctgtgtgaa ttgatcgaga acgatctggc aatggtcttg accgagcgct tcgagcaggt
      421 gcagcgcggg gaagcactac ctgccgcgcc cgtgacgcgc gccagccatg tgccgcgccc
      481 gccgacgccg ctcgtcggac gcgagcggga actcgccgct gtgaaactca tgctggcgcg
      541 cagcgacgtg ggcctgctga cgctcaccgg gccaggtggc accggcaaga cgcgtctggc
      601 gatccaagct gcgctcgatc tgatcgacag cttcaagcac ggcgtgtcct ttgtcgagtt
      661 ggcgccgatc ggcgatccca agctggtccc gacagccatc gctcaggagc tggacattcg
      721 cgaagctcgc gatggccggt cgatcctgga gaacctgaag gattatctgc gtgacaagca
      781 cgtgctgctg gtgctggaca acttcgagca ggtcgtgtcg gcggggccgg tcgttgtgga
      841 gctgctggga gtcagcccca ggctcaaggt gctggtgacg agccggacac tgctgcgtgt
      901 gcgcggcgag caagagatgc ccgtcaggcc gctcgaagtg ccggagcgca ggcgctcgct
      961 cgacgccgaa cgcctgtcgc agtatgcttc ggtcgagttg ttcattcagc gcgcgcaggc
     1021 ggcgaagccc gacttcgaga ttacgaacga gagcgcgccg gccgtcgccg agatctgcta
     1081 ccggctggac gggctgccgc tggcgatcga actggcggcg gcgcgcatca gaatcctcac
     1141 gccgcaggca ctgctcgcgc gcctggagcg ccggctggac gtcttgcgcg gtgggccgcg
     1201 cgacctcccg gcgcgccaac aaacgctgcg cagcacgatc gattggagtc acaacttgct
     1261 ggacaatcac gccaaggtgt tgttcaggcg gctgtcggtc tttgtcggcg gatggacgct
     1321 tgaagccgcg gaggcagtct gcaatgcgac aggcgatctc gacgttgaca ccctggacga
     1381 gatggagtcg ctgatcgata acagcctgct gaagccgacc gaggaagcgg ccggcgagat
     1441 gcgattcggg atgctggaga cgatccgcga gtacgcccgc gaacgcctgt cggagagcgg
     1501 cgaggaggac agcttgcgcg agcggcatgc cgagtatcat cttgcgcttg cggaagcgat
     1561 ccagccgagg ctgcttacct cagaacggcg gcggtggctc gatcgactgg agatagagca
     1621 cgataacatg cgagccgcgc tggattgctg caggctgggc cgatgtgatg cgtcggtcgg
     1681 cttgcgcctg agcgcggcgc tggcatggtt ctggcagaca cgtgcctatc ttcacgaggg
     1741 gcgaaagcac cttgaggctg tgctggcgcg cccggatgcg gcggcgcgca ccgcggcgcg
     1801 cgcgaacgcg ttatatggcg ccggcgtact cgcgtggtcg ctcggcgact acgccgtagc
     1861 ccgcgcgtgc ggcgaagaga gcgctgccat cgctcgcgac gtgggcgacc agcgccaggc
     1921 agcctacgcg ctcacccagc tcggcctggt gatcgccagc cagggtgata ctgaaaccgc
     1981 ctcggcattg tacgcggagg cggtgaagct gtttcgagaa gtgaaggacg cttggggaga
     2041 agcatttgcg ctcacctggc tggccgaggc gacacttgtg tcggaccacc cgctcatggc
     2101 gcagccgctg tacgaagagg gcctggcgct gtggcgcaga gtcggcgacc cgtgggggac
     2161 aggcatcgcg ctcctggtac tgggcgccct cgcgttggtg cagagtgacg cggcgaaagc
     2221 gtatgcgctg gtcgaagaag gcatgacact cttgcgggag gcaggagatc gctggacttt
     2281 gtccttcggc attgccggat taggcgatgt gctgttatgg cagggtgact atgcgcaggc
     2341 gggggcgcaa ttcgcggaag gcttgaggct ctcgcgggaa tcggggaacc cgcttaccat
     2401 ggtcatgagt ctggttggcc tggcgggggt gaccgccggg cgcagcctgc gcgagtcccc
     2461 ggagcagcgc gatgaaaatc tgcgaaggac agcgacgctg gtcggcgcgg ccgacgcact
     2521 gcgtgaggcg ctgggccttc acctgttccg cgtcctgcgt ctgacttacg aacctcgagt
     2581 ggctttgatt cgagcccagg ttgatgaggc catctggggg ccagcctggg ccgaaggcca
     2641 cgccatgagc ctggatcgcg cgatcgccta cgcgctggag aaagaacctc atgcctgaaa
     2701 gcaagtccgt gatccgcacc cccgaccagc ggctgcgcgt gttcgtcagc tccacgctgc
     2761 aagagctggc agaagaacgc gtggccgcgc gccaggcgat tacccatctg cggctggcgc
     2821 cggtgctgtt cgagctgggc gcgcgcccgc acccgccccg cgagctgtat cgggcctatc
     2881 tcgatcagag ccacatattc gtcggcgtct actggcagaa gtatggctgg atcgcaccga
     2941 gcgagacgat ctcagggctg gaggatgagt accgtttgtc gggcgagcgg ccgaagctga
     3001 tctacgtcaa gacgcccgca cccgaccgcg agccttgcct gaaggctctg ctcgatcgca
     3061 tcaggagcga cgacacggcc tcctataagc cgtttacaac ggctgccgaa ctgcgcgatc
     3121 tgatcgagaa tgatctggcc atgatcttga cggagcgctt cgagtcggct cacctggccg
     3181 cgggtgcgcc gagcgaagag gcgactcgcg cgcaaggcaa tttgcccgtc gtgcgcacgc
     3241 cgctcgtcgg gcgcgcgcgc gagctggccc tgacacgcga gttactgctg cgcgaatcgt
     3301 cgccggcgcg attggtgacg ctgaccggac ccggcggcat cggcaagacc cggctggcgc
     3361 tggaagccgc caacagcctc ggcgaatggt tctcagacgg tatctatctg atcagcctgg
     3421 cgccgctcac cgatccaagc ctgattgttc cagcgatcgc acagaccctc ggcatgcgcg
     3481 agacggccga ccgcccgctg cgcgacaatt tgatcgacta tcttcacgac aaacggatct
     3541 tgctgctgtt ggacaacttc gaacacgtcg tggccggcgc cggggccgtc ggtgaattgc
     3601 tcgaagcctg ccccctgctg aagatcgtcg ctactagcca gacaccgctg cgcatccgtg
     3661 gggagcgtga gctgcccctg ccgccgctgg cgctgccgcg catcggcgcg ctggcggccc
     3721 gctctggcga catcgtgtct caactgtccc aatacgctgc cgtggagctg tttattcagc
     3781 gggcgcggga tgtcaggcct gacctcagcg tgacgaacga gacggcgccc gccgtcgccg
     3841 aaatttgcta ccggctcgac ggcttgccgc tggcgatcga gctggcagcc gcgcgcatca
     3901 aggtgctgtc gccgcaggct ctgctggcgc gcctggagcg ccggctcgac gtgctcaaga
     3961 ggggcgcacg tgacctgccg gcgcgccagc aaaccatgcg cggggcactc gactggagcc
     4021 acgatctgct ctgcgaaagc gaaagaacgc tgttccggcg tgtgtcggta tttgtcggcg
     4081 gctgcacgct ggaagccgtc gagttcgtat gcgtgggtct gggcaccgac gaagtcgatt
     4141 tgctcgatca gttgacgtcg ctggtcgaca agagcctgct cgtgcaggat gagatgcccg
     4201 gcggcgagcc tcgcttccga atgctgcaaa tgattcaggg gtacgcgcgc cagaaactgg
     4261 tcgagtccgg cgaggcgaat gtctttctcc ggcgccatgc cggtttctac tgtatgatgg
     4321 ccgaagaggc tgagccttat ttgatgggcg cggcgcgcac ggcgtggctg gatcgtctgg
     4381 aaatggagtt agataacctg cgtgcggctc tggactggag ccgacagtcg ggcgccgaca
     4441 tgctcttgag actgtccggc tcacttgcat ggttctggtt catgcgcggg cacttgagcg
     4501 agggccgcgc gtggctggac agcgccatcg cctgcgcgcc gcgcctggcc gccacggcgg
     4561 acgcggcgcg cgccagggcg ctgtgggcgg caggcgggtt ggcttgggca cagggagatt
     4621 acgcccgtgc gcgtctcctg atccaggaga gcctggcgat ttatcgagag gtgcatgacc
     4681 ggcatgggag cgcgcatgcg ctatcgtttc tcggactggt catgttgagc ctagccgagc
     4741 cgagtgaggc gcatcggctg tgcgaagaag ctgtgtcgct ctcgcgggaa gtgaacgaca
     4801 agtggggtga agcgttcgca ctccgctgca tgggcgacgc cgcgctgctg ctgggcgatg
     4861 tcgtcggcgc gcgcgcgctg tacgagacca gcctcgggct atggcgcaag gtgaacgatc
     4921 cgtggggcat ctccatgccg ctcaacgacc tgggacgcgt cgcctatgtg cgcgaggatt
     4981 acgcggaggc gcgcgctgcc tacgaggaaa gcgtcgcctt gctgcgtcgc gtcggagaca
     5041 aatggggact cgccttggtg ttgaccaacc tggcaaacgt gctgattcat caggatgtgc
     5101 aggcggcgcg caccgtcctc caagagtgcc tgagcacgtg gcgcgagctt ggcaacaaaa
     5161 ccggcgtggc gcagtgcctg gctgcgtatg ccggactcgc cgctgtcgag cgccggccgg
     5221 cgctggctgc gcagttgttc ggcgccgccg atgcgcagtt aggggccatc ggctatcgac
     5281 ccgagggcat gttccgtgcc cagatcgaac gaaaccgcag cgtcggccgc gctcagctcg
     5341 acgagactac ctggcgcgcg gaatgggagc gcgggcgggg cttgcccacc gacgaggcta
     5401 tcgcgttggc gatgcagctt gagcaactgc ctactcagat agaatagtct gacaggctac
     5461 tgagtcggaa gaggcgtgag cagacttccg aagtctcaaa gacttcggaa gtctctcttg
     5521 tcaggccgat gcgccggagt aatgccgcag tccaccgtct cagagcttcg ttgatgacca
     5581 ctttgtcaga acgtcatggg tagagccacc aggcgttgcg cgctcgtggg tcttgtagcc
     5641 tgcctgcgtc gcggatgctg atgccgattt cggtgccgat ggcgcgcccg actggctggc
     5701 ttcctgccag ggccggtagc tgctgccgcc cggcggcatt caacgcctgg tacagcgcga
     5761 gcaggcttgg ctctaagcaa atgttttcca ggtagagcgt gtagccggcg acgatgtcat
     5821 cttcgcgcgt gtacttggcg acgaggcgcg tctggctggc gtacaggacc atcgcatcat
     5881 agccggggct gatctcgtag cccgagtccg gcaaatggat gatctcgcct ggcgcggtgg
     5941 ccagccctgc gacggacacc gccggtttcg tcacgatttg cgtcgatggg cagttgcagg
     6001 tcgtgtccca tctgtacagc gcgtacaccg tgctgatcgc aggcgtgcgc acgtcggcga
     6061 acagatagcg gaactgtggc gcgttcggat cgctccccgt gccgatgtcg aagatcgcct
     6121 taagctcgtc gacgggcgtg tagccgcgca gagccagatt gatgcggtaa tcgttctgcg
     6181 gatcgatgcc gatcaccggg tcgggcgcga cggcgccgta cgaaacgccc ggtatgggag
     6241 cgcacccgga tttatgaatg gccggaaggt aaa
//
LOCUS       DBNHXB010000044         4486 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3867212, whole genome shotgun sequence.
ACCESSION   DBNHXB010000044 DBNHXB010000000
VERSION     DBNHXB010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3867212"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="AC162G_01715"
     CDS             <1..1207
                     /locus_tag="AC162G_01715"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR5074565.1"
                     /translation="AGIRAGEGDVIPVGQTIAWILAPGETVPSVPESIPSVQSVPESV
                     SMATVSASPLARRIAEEHGIDIARVKPGGGRVEKADVLAYLTPHPGLAATPPPSPNLE
                     DWERGEGRGRGVRVLASPKARRLAVERGLDLATLYGSGPGGAVLAADVPTTVPLVPSV
                     SGAAVPAVPAPPSGEIESLGTVWRVMAERMTGSWTSVPHFYLMREIVASGLVDMRARI
                     LPAVEKRSGIKPSYTDLLVKLVATALRDHPRLNASWVDGGIRLNREINIGIATAIEEG
                     LIVPVIHGAQGKSIGEIAARRNDLVMRANENKLRLNDIAGGTFTITNLGMYNVDVFNA
                     IVNPPQAAILAVGRITDRVVAVNGQPAVRPTMIVTLSCDHRVVDGARAAKFLDDLANL
                     MEEPWGVLA"
     gene            1204..2703
                     /locus_tag="AC162G_01720"
     CDS             1204..2703
                     /locus_tag="AC162G_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5074566.1"
                     /translation="MTTLERLRTAAPVAAAKIGDETPALGEYGCFVGGSWLKTGHAVE
                     VRSPYDESLVAIDHRAGPKEIEAAIAVALRAFQLTRKLPSWKRADVLEKISAGIAARR
                     EDFARTIALEAGKPIRTARAEVDRAIFTFKVAAEESRRIYGEIVPLDWIPGTEGREGI
                     MRRVPLGPIAGISPFNFPLNLVAHKVAPALAAGDPIIIRPASQTPVSALKLAEVISES
                     GWPEGAFSVVPSTTEDAAPLVEDDRIRLLTFTGSPAVGWALKRRAGRKRVTLELGGNA
                     GVIVHHDADLRYASERVAWGGFSYAGQSCISVQRVYAHAEIVEAFLDDLLPRVKALKV
                     GDPLDEATDIGPLIDRSAAERVEAWVNEAKQGGVEVLAGGTRQGNLWQPTVLAAIQPA
                     MRVSCQEVFAPLVGVYRYTDVRQAIAAVDDSDFGLQAGLFTNDWNVIQAAFDDIEVGG
                     LMVNDVSTFRIDHMPYGGAKQSGMGREGLRYAIEEMTEMKLLAINRRGL"
     gene            2838..4121
                     /locus_tag="AC162G_01725"
     CDS             2838..4121
                     /locus_tag="AC162G_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5074567.1"
                     /translation="MKRTHLLVAGTIILSFLLVACGGPATTAPPVPTQPPVATQPPAP
                     TQPPPTEAPTEVPPIRIGASLPLTGDFSEPGTAAMRGYEVWVEMVNEAGGLLGRPVEL
                     IVLDNASDQDTAVADYEKLITVDKVDLVVGPFSSRLVIPTSEVAARYGYAFVEPAGGA
                     PDVFNRGLTNLFFAQPARGAEQADPFADYILSLPEDVRPKTFAVVSQDDPFALGVVER
                     LKEQLTEGGLELVFDEIYAPDTTDFSAIAAQVADLDPDLLVGGTILEDSIGQIRAYQE
                     AGYQPRGAFFTTGPSLPGPFREALGDATEGVFAAMSWFPEAEEYQNAEFVARYNEKFG
                     GTLADIPEDAANAFTVGQVLEQAVGNTDNIDNAELIEELHEGVYQTVVGPLSFDEVGA
                     PKGSFMILQWQGDTFVIVGPPGREQAEPIWPKPEW"
     gene            4221..>4486
                     /locus_tag="AC162G_01730"
     CDS             4221..>4486
                     /locus_tag="AC162G_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5074568.1"
                     /translation="MNLLSTNVLIQVAIGGLLTGGVYALMAGGLSLIFGVLRVINIAH
                     GAFLVFSAYIAYWLFTLYGLDPILSILITIPLFFLIGAVVQRFML"
BASE COUNT          793 a         1416 c         1502 g          775 t
ORIGIN      
        1 cgccggcata cgcgcgggcg agggcgatgt gatcccggtg ggacagacca tcgcctggat
       61 tcttgcgccc ggtgaaaccg tcccatccgt gcccgaatcc ataccatccg tgcaatctgt
      121 gcccgaatcc gtgtctatgg cgacggtcag cgcttccccg cttgcgcgcc ggatagcgga
      181 ggagcacggc atcgacatcg cgcgggtgaa gcccggtggc ggacgtgttg aaaaggcgga
      241 cgtgctggcg tacctcaccc cccaccccgg cctggcggcc acccctcccc cctctcccaa
      301 tctggaggat tgggagaggg gggaggggcg ggggaggggg gtgagggtgc tggcctcccc
      361 caaagcgcgg cgactggcgg tcgagcgcgg cctcgacctg gcgacgctgt acggctccgg
      421 cccaggcggc gcagtgttgg cggcagatgt ccccaccaca gttcctttag ttccttcggt
      481 ttctggcgca gccgtgcctg ccgtgcctgc tccgccgtcg ggcgaaatag agtctctcgg
      541 cactgtctgg cgcgtcatgg ccgagcgcat gaccggtagc tggacgagtg tgccgcactt
      601 ctatctgatg cgcgagatag tcgcgagcgg gctggtcgac atgcgcgcgc ggatcctgcc
      661 tgccgtcgaa aagcgcagcg gcatcaaacc gtcctacacc gacctgctcg tcaagctggt
      721 cgcgaccgcg ctgcgcgatc acccacgcct gaacgcgagt tgggtcgatg gcgggatccg
      781 gctgaatcgc gagatcaaca tcggtatcgc gacggcgatt gaagagggct tgatcgttcc
      841 ggtgattcac ggcgcccagg ggaagagcat tggcgagatt gccgcgcggc gaaacgacct
      901 ggtgatgcgc gcgaatgaaa acaagctgcg cctcaacgac atcgccggcg gcacgttcac
      961 catcacgaat ctgggcatgt ataacgtcga cgtgttcaac gccatcgtca atccgccgca
     1021 ggccgccatc ctggcggtag gccgcatcac ggatcgagtg gtggcggtga acggccagcc
     1081 cgccgtgcgc ccgacgatga tcgttacgct ctcgtgcgat catcgcgtgg tggacggcgc
     1141 gcgcgcggcg aagtttctgg acgatctggc aaacctgatg gaagaaccgt ggggggtact
     1201 ggcatgacaa cactggaacg ccttcgcact gccgctccgg tcgcagcggc gaaaatcgga
     1261 gatgaaaccc cggcgctggg agaatatggc tgcttcgtcg gcgggtcgtg gttgaagaca
     1321 ggccatgccg tcgaagtccg cagcccgtac gacgagtcgc tcgtggcgat cgaccatcgc
     1381 gcaggcccga aggagatcga agcggccatc gcagtcgccc tcagggcttt ccagctcacg
     1441 cgcaaattgc cttcgtggaa gcgcgccgac gtgctcgaaa agatcagcgc gggcatcgcc
     1501 gcgcggcgcg aggacttcgc ccgaacgatc gcgctcgaag ccggcaagcc gatccgcacc
     1561 gcgcgcgccg aagtcgatcg tgcgatcttc accttcaaag tggccgccga ggaatccagg
     1621 cgtatctacg gcgagatcgt cccgctcgac tggattccgg gaaccgaggg gcgcgaaggc
     1681 atcatgcgac gcgtgcccct cggcccgatc gccggcattt cgcccttcaa cttcccgctc
     1741 aacctcgtcg cgcacaaggt cgcgcccgcg ctggcggcgg gcgatccgat catcattcgt
     1801 cccgccagcc agacgccggt gagcgcgctc aagctggccg aggtgatttc cgagtcaggc
     1861 tggccggaag gcgcgttcag cgtcgtgccc agcaccacgg aggacgccgc gccgctcgtg
     1921 gaagacgacc gcattcggct gttgaccttc acgggcagcc cggcggtcgg gtgggcgctc
     1981 aagcgtcggg cagggcgcaa gcgagtgacg ctcgagcttg gcgggaacgc tggcgtgatc
     2041 gtccaccacg acgccgatct gcgttacgcc agtgagcgcg tcgcgtgggg tgggttctcg
     2101 tacgcagggc agtcgtgcat ctcggttcag cgcgtgtacg cgcatgccga aattgtcgaa
     2161 gcgtttctcg acgatctcct gccgcgcgtg aaggcactga aggtcggcga tccactcgac
     2221 gaggcaaccg acatcgggcc actgatcgat cgcagcgcgg cagagcgcgt cgaagcctgg
     2281 gtgaacgagg cgaaacaggg cggcgtggag gtgctggccg gtgggacgcg ccagggcaac
     2341 ttgtggcagc cgaccgtgtt ggccgcgatt caaccggcga tgcgcgtgag ctgccaggaa
     2401 gtatttgcgc cgctggtcgg cgtgtatcgc tacacggacg tgcggcaggc gatcgccgcc
     2461 gtggacgaca gcgacttcgg tctgcaggcc gggctgttta ccaacgactg gaacgtgatc
     2521 caggccgcgt ttgacgacat cgaagtgggc gggctgatgg tcaacgacgt gtccacgttc
     2581 cgcatcgacc acatgccgta cggcggagcc aagcaatcgg gcatggggcg tgaggggctg
     2641 cgttacgcga tcgaagagat gaccgagatg aagctgctgg cgatcaatcg acgcggcctg
     2701 tagccgtagc tgcactccgg caatttgcca aagttcagga gtaaagaaag gaggagacgc
     2761 tcgacgtcga cgcagtcaac tttcaagcta gtgcaacccc gatccggtca acaacattca
     2821 gagaggagtg aggagagatg aaacgaaccc atttgctcgt cgcaggaacg attatcttgt
     2881 cttttctgct ggtggcctgc ggaggtccgg cgaccacggc cccgcctgtg cccacacagc
     2941 ctcccgtcgc gacacaaccg cctgctccga cccagccgcc gccgacagaa gcgcctaccg
     3001 aagtccctcc cattcgtatc ggcgcatcgc tgccgctcac cggcgatttc agcgaacccg
     3061 gcaccgccgc gatgcggggc tacgaggtct gggtggagat ggtgaatgag gccggcggcc
     3121 tgctgggccg gccggtcgag cttatcgtcc tcgataacgc cagcgaccag gacaccgccg
     3181 tcgccgacta tgaaaagtta atcacggtgg ataaagtgga cctggtcgtg gggccattct
     3241 ccagtcgcct ggtgatcccg acctccgagg tggccgcgcg gtacggctac gcgttcgtcg
     3301 agccggccgg gggcgcgccc gatgtattca accggggcct gaccaatctg ttctttgcgc
     3361 agccggcgcg cggcgcggag caggccgatc cgtttgccga ttacatcctc agcctgccgg
     3421 aggatgtgcg gccgaagacg ttcgccgtcg tgagccagga cgatccgttc gcgctggggg
     3481 tggtggaacg gctcaaggag cagctcaccg aaggcgggct ggagcttgtc ttcgacgaga
     3541 tctacgcgcc agacacgacc gacttctctg ccatcgcggc gcaagtggcc gatctcgacc
     3601 ccgatctgct cgtgggcggc accatcctgg aagattcgat cggccagatt cgcgcctacc
     3661 aggaagccgg ttatcagccg cgcggcgcgt tcttcacgac cggcccgtcg ctgcccgggc
     3721 cgttccgcga ggcgctgggc gacgccacgg aaggcgtctt cgcggcgatg tcgtggttcc
     3781 cggaggcaga agagtatcag aatgccgagt tcgtcgccag gtacaacgag aagtttggcg
     3841 gcacgctggc cgatattccc gaagacgccg cgaatgcctt caccgtcggc caggtgctgg
     3901 agcaggccgt ggggaatacc gataacatcg acaacgcaga gctgatcgaa gagctgcacg
     3961 agggcgtata tcagaccgtc gtcgggccac tcagctttga cgaggtcggc gcgccgaaag
     4021 gctcgtttat gatcctgcag tggcagggcg ataccttcgt catcgtcggc ccgcccggcc
     4081 gcgaacaggc tgaaccgatc tggcccaagc cggagtggta gacctcaccc cctcccccaa
     4141 cccctccccc tctcctgtgc gttgcgcaca ggagaggggg aggggagcgg agcggtgagg
     4201 gggtgaggcg cgagacgcgc gtgaatcttc tatccaccaa cgtattgatt caagtcgcca
     4261 ttggcgggct gctgaccggc ggcgtgtacg cgttgatggc cggtggcctg tcgctgatct
     4321 tcggcgttct gcgcgtgatc aacatcgcgc atggcgcctt cctggtgttc agcgcctaca
     4381 tcgcctactg gctgttcacg ctgtacggcc tcgatccgat cctatcgatc ctgatcacta
     4441 tcccgttgtt tttccttatc ggagccgtcg tgcagcgctt catgct
//
LOCUS       DBNHXB010000045         7804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3196506, whole genome shotgun sequence.
ACCESSION   DBNHXB010000045 DBNHXB010000000
VERSION     DBNHXB010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3196506"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="AC162G_01735"
     CDS             complement(<1..163)
                     /locus_tag="AC162G_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR5074569.1"
                     /translation="MSNGHFDRTKPHVNVGTIGHIDHGKTTLTAAITKTLALKKMAEF
                     RSFDSIDNAP"
     gene            complement(242..314)
                     /locus_tag="AC162G_01740"
     tRNA            complement(242..314)
                     /locus_tag="AC162G_01740"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(279..281),aa:Thr,seq:ggt)
     gene            complement(839..911)
                     /locus_tag="AC162G_01745"
     tRNA            complement(839..911)
                     /locus_tag="AC162G_01745"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(876..878),aa:Thr,seq:tgt)
     gene            1039..1111
                     /locus_tag="AC162G_01750"
     tRNA            1039..1111
                     /locus_tag="AC162G_01750"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1073..1075,aa:Glu,seq:ttc)
     gene            1240..2187
                     /locus_tag="AC162G_01755"
     CDS             1240..2187
                     /locus_tag="AC162G_01755"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5074570.1"
                     /translation="MHYRNLGRSGLKVSAVGLGTNRFGNKCDLDTTRRILHRALDLGV
                     NLVDTADVYAGGKSEEFIGESIRDRRDRYVIATKVRSGVGDGPNDAGASRYHILNGVE
                     ASLRRLQTDYIDLYQIHSWDDETPIEETMRALDDLVRMGKARYVGASNFAAWQLCRAN
                     DLAEMHRWTAFASVQPEYHMLERGIEAELIPYCIAFDVGILPYFPLAGGFLTGKYKRG
                     QGAPPGSRGESSTYVQKYMTGANYTSIERLTQFAETRRHTITELAVAWLLAQPQVSSV
                     ISGATKPEHVEANVKGAEWVLSEDEVSEVDGILSVKREA"
     gene            2243..3997
                     /locus_tag="AC162G_01760"
     CDS             2243..3997
                     /locus_tag="AC162G_01760"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5074571.1"
                     /translation="MNTEFAITLDWRSDQRTPLRWVFSHLLRSKLLILIVIVGAFGNA
                     ALAAAVPLYTGIAFDAILKLPPDLATVGAAALAIVISQAVRGVLQFGRNYGNEIIGQR
                     LERDARAELYTSLLGKSMTFHDRQSTGDIMARATNDVREMNLMLNPGINLVIGSAMFL
                     VVPILAAPRIHPQLILVPLLFCAGYFLALWRFLVELRPVTERVRRRFGGMNAILAETI
                     EGIETVKGAAKEEYEIARFDEGATAYRDAFVRQGDIEARFVPLLLMGLATGAGLFQAL
                     ALYRVGEISVGDIVAYMGLLGLFGFPTFISLFAYAQISTGFASARRILELIHVKTELD
                     ENKEGHAAPMQGAIRFEHVSFFYRGKAQAVEDVSFEVRPGQTLAIVGQTGSGKSTIIK
                     LINRIYDANAGRVLIDGVDVRDWNLESLRRQISIIEQDLFLFSRTIAENIAFGCPGAT
                     REEIERAAQEAQAHEFITSFADGYDTVVGERGVTLSGGQRQRIAIARAFLTDPRILIL
                     DDSTSAIDSATEDQIQQAIERASQNRTTILITHRLSQIRWADVILVMRNGRVAAIGTH
                     EELMRTSESYRRIFARYE"
     gene            4073..5920
                     /locus_tag="AC162G_01765"
     CDS             4073..5920
                     /locus_tag="AC162G_01765"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5074572.1"
                     /translation="MFAGLDAEAYDREYSDRVLIRRIALYFAAYRGKVVSISLLVAAM
                     AIFGAALPIVIARGVDALEEQPPGLLYVALPAFVALVGVLNWAANWARRRLTQRVTGD
                     VMLSLRRDAFRASVQHDLSFYDEFKTGRIVSRITSDTQEFAQVVVLVTDLISQVLVVL
                     ILLPILFSIEWRLTLLLLATTPVVYVVAAGFRKLARHVTRQGSRAMAEVNAAIQEAVT
                     GIHIAKNFRQEQAIYESFNRVNALSYRINLRRGMVLATIFPVLNALGGVGTALLVYFG
                     GQAAVAGAITAGAWFLFVTSVDRFWFPMINIAAFWSQFQGGLSASERIFALIDAEPVV
                     RQIDNRPAPKLRGEICFDRVNFRYSKQETVLEDFSLCIAAGENVALVGHTGAGKSSIA
                     KLIARFYEFQSGRLTIDGHDIRSFDLQSYRRLLGIVSQTPFLFSGTVADNIRYGSLEL
                     SDGDIEAIARRIGGGEWLDALPDGLRADVGERGDRLSMGQRQLVALTRVLAQQPAIFI
                     LDEATASVDPFTESQIKEALDLILAGCTSILIAHRLSTVKSADRIIVLREGEIIEEGN
                     HAALMAQGGHYAELYDTYFRHQSLEYVLSGPKQRAPVFAREARAAVIRK"
     gene            6033..6380
                     /locus_tag="AC162G_01770"
     CDS             6033..6380
                     /locus_tag="AC162G_01770"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR5074573.1"
                     /translation="MPESIDDTFWRRRLENELKRYVPILVAQYEPERIILFGSLAADR
                     AHAWSDIDLVVVGHTEKRFLDRTKEALLLLRPIVGLDILIYTPEEFDQLSQERQFFKH
                     EILRGKVLYERSR"
     gene            6364..6765
                     /locus_tag="AC162G_01775"
     CDS             6364..6765
                     /locus_tag="AC162G_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009768125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR5074574.1"
                     /translation="MSEAADWLRFATEDLQVAEWALSSRIYNQVCFHAQQCVEKALKA
                     WLIHHHLVSPRAHQMSALLGVAPRPLPFSAEVEEGVVGLDRFYIPTRYPDALPGMLPE
                     GLPSAADADEALTLARQVFTAVSESIERSQA"
     gene            6898..7302
                     /locus_tag="AC162G_01780"
     CDS             6898..7302
                     /locus_tag="AC162G_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR5074575.1"
                     /translation="MEELEITVSQEQGRAPVTVFHLKGRMNLGTVEQLQAKAQETTQA
                     GARYMLLDLTGVQSLTSAGLRVIHQIYQLLRDASTAGGDEAARKAQAGALKSPHLKLL
                     NPTPDVRRVLQIAGFDMFLEIHDDKKDAVASF"
     gene            7503..>7804
                     /locus_tag="AC162G_01785"
     CDS             7503..>7804
                     /locus_tag="AC162G_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074576.1"
                     /translation="MSSAETLNIVGYRNEAVPNTFFRQKGETRHVAILFPGLGYTAHM
                     PVVYYPGRLLAARGADVLRVEYTYNRRPDFQALPDEEQDRWFHADVTAACDAALAQR"
BASE COUNT         1490 a         2436 c         2378 g         1500 t
ORIGIN      
        1 ccggcgcgtt gtcgatcgag tcgaacgacc gaaactccgc catcttcttc agcgccagcg
       61 tcttggtgat cgccgccgtc agcgtcgtct tgccgtggtc gatgtgcccg atcgtcccca
      121 cgttcacatg cggcttcgtc cgatcaaaat gcccgttcga catgaatgtc gacctccctg
      181 atgtttagaa aaactgccgc gcttgcgcgc gggggtaggt aacgctctgc tctgcatgca
      241 aagcccacga tgggaatcga acccatgacc acacccttac caagggtgtg ctctaccgac
      301 tgagctacgt gggcacattg cgaccgcgcc aatctccggc aaccggctga gcatggaaat
      361 tatagcgaaa atcccaaaat gcgcaagatg cggaacgcat gttacacaca aatgcctcgc
      421 ccctctgaga ggggcgagtg ggcggtgaca gattcgaact gccgaaggca tttgccagct
      481 gatttcgtcc cagctacagc tttcgctgcc agcgctgcgc tgtttgtggg ctggactttg
      541 tcttcatccg ttctggatgc tccccgtcaa gtctctacac cttgccccga tatgggacct
      601 ggctcggcgt tgccactccc agaggtttcg ccgaatttgg ggagttttca cctgcggatt
      661 cctccgcagg gctgcaacta tcgcctgtgc ctggaagcaa atacctcact ggcgagtgcc
      721 aggttgcctt tctgctgatt attcattgct ccgggcaacc tgactccttc acagtcagcc
      781 ccctttgtcc acttgggtaa ccgcccaggg tgcgacatca cgacgcgacc cgcctccaag
      841 ccgacagcgg gaatcgaacc cgcgacccat cgcttacaag gcgattgctc tgccggctga
      901 gctatgtcgg cgcttgctaa cccatcgcgc gtggattata ccattggcat cgaggcgggt
      961 caagcaattc tcgaatcttg accgatcaga tcaaggctgg tagaatcgac tcgcttcagt
     1021 tcgtcgactg gcgcggctgc ccccatcgtc tagtggacca ggacgtggcc ctttcaaggc
     1081 caagaccggg attcgaattc ccgtgggggc actgtgcaac ctgtccatct cctcttgaag
     1141 tgcctgcttc aagaggattt tgttgtgccc gacgcacagt tatttaacgg aatgatataa
     1201 tcgtcactct catacttcca cacccggagg agccaacaca tgcactaccg caatctcgga
     1261 cgctccggcc tcaaagtatc cgccgtcggg ctgggcacga accgttttgg caacaagtgc
     1321 gatctggaca cgacgcggcg catcttgcac cgcgcgcttg acctcggcgt gaacctcgtc
     1381 gacaccgccg atgtgtatgc cggcggcaag tcggaggagt tcatcggcga gtcgattcgc
     1441 gatcggcgtg accggtacgt gattgcgacc aaggtccgct ccggagtggg ggatggcccg
     1501 aatgacgccg gcgcatcgcg ctaccacatc ctcaacggcg ttgaggccag cctgcggcgt
     1561 ttgcagaccg actacatcga tctgtaccag attcacagtt gggatgacga gacgccgatc
     1621 gaagaaacga tgcgcgcact ggatgacttg gtgcgaatgg gcaaggcgcg ctacgtcggc
     1681 gcgtcgaact tcgccgcgtg gcagctctgt cgcgcgaatg acctggctga gatgcatcgc
     1741 tggacggcgt ttgcctcggt tcagccggaa taccatatgc tcgagcgggg catcgaagcc
     1801 gagctgattc cgtattgcat agcgttcgac gtcggcatcc tgccctactt cccgctggcc
     1861 ggcgggttcc tcaccggcaa gtacaagcgc ggccagggtg cgccgcccgg atcgcgcggt
     1921 gagagcagca cgtacgtgca gaagtacatg accggcgcaa actacacgag catcgagcgc
     1981 ctgacgcagt ttgccgaaac gcgcaggcac acgataaccg agcttgccgt cgcctggctg
     2041 ctggctcagc cccaggtctc gtccgttatc tctggcgcga cgaagcccga gcacgtcgag
     2101 gcgaatgtca aaggggcaga gtgggtgttg agcgaggatg aagtgagcga agtcgacggg
     2161 atactgagcg taaaacgtga agcgtgaaac gtaaggcgat cagccatccg ccacctgcta
     2221 tctgccatct gcgatcagcc ccgtgaacac agaattcgca attacactcg attggcgctc
     2281 cgatcagcgc acgccgctgc gttgggtctt ctcgcacctg ttgcgcagca agctgctgat
     2341 tctgattgtg atcgtcggcg cgttcggcaa cgcggcactc gcggcggcgg ttccgctgta
     2401 caccggcatc gccttcgacg cgatcctgaa attgccgccc gacctcgcca cggtgggcgc
     2461 cgctgcgctg gccatcgtca tctcccaggc cgtgcggggc gtcctgcagt tcgggcgcaa
     2521 ctacggcaac gagatcatcg gccagcggct ggagcgcgac gcgcgcgccg agctgtacac
     2581 cagcctgctc ggcaagagca tgacgttcca cgaccggcag tcgaccggcg acatcatggc
     2641 gcgtgcgacg aacgacgtgc gcgagatgaa cctgatgctc aacccgggca tcaacctcgt
     2701 catcggatcg gcgatgttcc tcgtggtgcc gatcctcgct gcgccgcgca ttcaccctca
     2761 actgatcctc gtgccgctgc tgttctgcgc cggctatttc ctcgcgctgt ggcgctttct
     2821 cgtcgagctg aggccggtca ctgagcgtgt ccgccgccgc ttcggcggga tgaatgccat
     2881 cctggccgag accatcgaag gcatcgagac ggtcaaaggc gcggcgaagg aagaatatga
     2941 gatcgcaaga ttcgacgaag gcgcgaccgc gtatcgcgac gctttcgtgc gccagggcga
     3001 catcgaagcg cgcttcgtgc cgctgctgct gatggggctg gcgaccggcg ccggcctatt
     3061 tcaggcgttg gccctatacc gcgtaggcga aatcagcgtc ggcgatatcg tcgcctacat
     3121 ggggctgctg ggcctcttcg gctttccgac cttcatctcg ctgttcgcct acgcgcagat
     3181 atcgaccggc ttcgccagtg cgcgccgcat cctggagctg attcacgtta agaccgagct
     3241 ggacgaaaac aaagagggtc atgccgcgcc gatgcagggc gcgatccggt tcgagcacgt
     3301 ctccttcttc tatcgcggca aggcgcaggc cgtcgaggac gtgtcgttcg aggtcaggcc
     3361 aggccagacg ctggccatcg tcgggcagac cggatccggc aagagcacca tcatcaagct
     3421 gatcaaccgc atctacgacg cgaacgcggg ccgcgtgctg atcgacggcg tcgacgtgcg
     3481 cgactggaac ctggaatcgc tgcgcaggca gatttcgatc atcgagcagg acctgttcct
     3541 cttctcgcgc acgatcgccg agaacatcgc cttcggctgc cccggcgcga cgcgcgagga
     3601 gatcgagcgc gcagcgcaag aagcgcaggc gcacgaattc atcacgtcct tcgccgacgg
     3661 ctacgacacc gtcgtcggcg agcgcggcgt gacgctgagc ggcgggcagc ggcagcgcat
     3721 cgccatcgcg cgcgccttcc tcaccgaccc gcgcatcctg atccttgacg attcgacgag
     3781 cgccatcgac agcgcgactg aggatcaaat ccagcaggcg atcgagcgcg cctcgcagaa
     3841 ccgcacgacg atcctgatta cgcaccgact gtcgcagatc cgatgggcgg acgtgatcct
     3901 ggtgatgcgc aatgggcgcg ttgcggcgat cggcacgcac gaggagctga tgcgcacgtc
     3961 ggagtcgtat cgccgcatct tcgcgaggta tgagtgacct caccccctcc ccctctcctg
     4021 ggcaaggccc aggagagggg gaggggacgg gggtgggggt gaggcagtgt ctatgttcgc
     4081 cggcctcgac gccgaggcct acgaccgcga atattcggac cgtgtcctga tacgccgcat
     4141 cgcgctctac tttgcggcat accgtggcaa ggtcgtgtcg atctcgctgc tcgtcgcggc
     4201 gatggcgatc ttcggcgccg cgctgcccat cgtcatcgcg cgtggagtcg acgcgctgga
     4261 agagcagccg cccggcctgt tatacgtcgc actgcccgcc ttcgtcgcgc tcgtcggcgt
     4321 gttgaactgg gcggcgaact gggcgcgccg tcgcctgacg cagcgcgtga cgggcgacgt
     4381 gatgctcagc ctgcggcgcg acgccttccg cgcgtcggtg cagcacgacc tgtcgttcta
     4441 cgacgaattc aagacggggc gcatcgtcag ccgcatcacg tccgacacgc aggagttcgc
     4501 gcaggtcgtc gtgctcgtca ccgacctgat cagccaggtg ctggtcgtgc tgatcctcct
     4561 gccgatcctg ttcagtatcg agtggcggct gacgctcctg ctcctggcga cgacgccggt
     4621 cgtgtacgtc gtcgccgccg gcttccgcaa gctcgcgcgc cacgtcacgc ggcagggatc
     4681 gcgcgcgatg gccgaggtga acgcggcgat ccaggaagcc gtgaccggca tccacatcgc
     4741 caagaacttc cggcaagagc aagccatcta cgaatcattc aaccgcgtca acgcgttatc
     4801 gtatcgcatc aacctgcggc gcgggatggt actggcgacg atctttcccg tgctcaacgc
     4861 actgggcggc gtggggacgg cgctgttggt gtatttcggt gggcaggccg ccgtcgccgg
     4921 cgcgatcacg gcgggcgcct ggttcctgtt cgtcacgagc gtggaccgct tctggttccc
     4981 gatgatcaat atcgccgcgt tttggtcgca gttccagggc ggcctatccg catccgagcg
     5041 catctttgcc ttaatcgacg ccgagccggt cgtgcggcag attgacaacc ggcctgcccc
     5101 taaactgcgc ggcgagatct gcttcgaccg cgtcaacttc cgttactcga agcaggagac
     5161 cgtgctggaa gatttctcgc tgtgcatcgc ggccggcgag aatgtcgcgc tcgtcggtca
     5221 caccggcgcg ggcaagagca gcatcgccaa gctcatcgcg cgcttctacg agttccagtc
     5281 cggccgcctg acgatcgacg gtcacgacat ccgctcgttc gatctccagt cataccggcg
     5341 gctgttgggc atcgtctcgc agacgccgtt cctgttctcg ggcacggtcg ccgacaacat
     5401 ccgctacggc agtctcgagt tgagcgacgg cgacatcgaa gcgatcgcgc ggcgcatcgg
     5461 cggcggcgag tggctggacg cgctgcccga tggcctgcgg gcagacgtcg gcgagcgcgg
     5521 cgatcggctg tcgatgggcc agcgccagct cgtcgcgctg acgcgcgtgc tcgcgcagca
     5581 gcccgccatc ttcatcctcg acgaggcgac ggcgagcgtc gatccgttca ccgagtcgca
     5641 gatcaaggaa gcgctcgatt tgatcctggc cggctgcacg tcgatcctga tcgcgcaccg
     5701 cctgtcgacc gtcaagtccg ccgaccggat catcgtgctg cgcgaaggcg agatcatcga
     5761 agaaggaaat cacgccgcgc tgatggcaca gggcggccac tacgccgagc tgtacgatac
     5821 gtacttccgg caccagtcgc tggagtatgt cctctcaggc cccaaacaac gcgcgccggt
     5881 gttcgcacgc gaggcacgag ccgccgtcat ccgcaaatga ggaaaccaca gatggtgcac
     5941 cctcattccc taaagcggtg tacaatcaag agcagaattc tccgcgggag atcggacggc
     6001 aattgatgac ggacgcaaaa ccggcgccag ctatgcctga atcgatcgac gacacatttt
     6061 ggcgccgccg cctcgaaaac gaattaaagc gatatgtgcc gatcctcgtt gctcagtatg
     6121 agccggaacg gatcatcctg tttggatcgc tggcagcaga tcgagcacac gcctggtctg
     6181 acatcgacct cgtggtggtg ggtcataccg agaaacgctt tctagatcgc accaaggagg
     6241 ccttgttgct gttgcgccct atagttggct tggatatctt gatttacacg cctgaagagt
     6301 ttgaccagct cagccaggaa cggcagttct ttaagcacga gatactcaga gggaaggtgc
     6361 tgtatgagcg aagccgctga ttggctgcgc tttgccacag aggatttgca ggtagccgag
     6421 tgggcgctat cgtcccgcat ctataatcaa gtctgcttcc atgcccagca atgtgtagaa
     6481 aaggcactca aggcctggct gattcaccat catctggttt ctccacgagc gcatcagatg
     6541 agtgccctgc ttggagtggc cccgcgccca ttgccgtttt cagccgaagt ggaagagggc
     6601 gttgtgggcc tcgaccggtt ctatattcca acccgctatc cggatgcgct tcccggcatg
     6661 ctgccggaag gactgccaag cgcagccgat gcagatgaag ctctgacgct agcgcggcag
     6721 gttttcacgg cggtgagtga gtctatcgag aggtcccaag cgtagtgtgg atatcggcgg
     6781 cagagcgaag gaagaatctt catgctctct gtgcctcagt ggctgtttct gatgtagaac
     6841 ctaccttcct tcgagaatga acgtataata gcgtgaaaag ggcaggggag gcgcatcatg
     6901 gaggagttgg agatcaccgt ctcgcaggag caaggccgcg caccggtcac cgtctttcac
     6961 ctgaagggcc gcatgaatct cggcaccgtc gagcagttgc aggcaaaggc gcaggagacc
     7021 acccaggctg gcgcgcgcta tatgcttctc gatctgaccg gcgttcagtc cctgaccagc
     7081 gccgggcttc gagtcatcca ccaaatctac caactgctcc gcgacgcttc gactgcaggg
     7141 ggagatgagg cggcgcggaa ggcgcaggcg ggcgcgctca agtcgcctca cctcaagctg
     7201 ctcaacccca cgcctgacgt tcgaagagtg ctccagatag cagggttcga tatgtttctg
     7261 gagattcacg acgacaaaaa agatgccgtc gcgtctttct aagtcagcct ccagcgcggg
     7321 ctgcgcccga tatcctatgg aatttgcaaa cagcaaatca gggagttatg caccgaaggc
     7381 tatatgtgag agttgtatcg agcgtcaact gttttctgtc ctcaatatag agacgaacga
     7441 gcgagccaaa tggtaacatc tcagacagac atcatcaaac aaggtgaaac ggagcgcatc
     7501 cagtgtcttc cgctgaaacc ttgaacatcg tcggctatcg aaacgaggcc gtccccaaca
     7561 cgttcttccg ccaaaagggc gagacgcgcc atgtggccat cctgttcccc ggtctcggtt
     7621 atacggcgca catgccggtc gtgtattacc ccggacgctt gctcgccgca cgcggcgcgg
     7681 atgtccttcg cgtggagtac acctacaacc ggcggccaga tttccaggca ctgccggatg
     7741 aggaacaaga ccgctggttt catgcagacg tcacggcggc atgtgacgcg gccctggctc
     7801 aacg
//
LOCUS       DBNHXB010000046         4857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2693799, whole genome shotgun sequence.
ACCESSION   DBNHXB010000046 DBNHXB010000000
VERSION     DBNHXB010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2693799"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="AC162G_01790"
     CDS             <1..411
                     /locus_tag="AC162G_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074577.1"
                     /translation="IRRENPADKYLVQARVTPRRTEAGPAWFRVLFALDEVLPFWWNV
                     QTHEYRPVTAEREACFGLSALRDIARTIARISRLDIFSTEIAQRAGDGVFVSIDYVND
                     PIDLRLQSKAPDGVPDPAVRLIAERIAGLADSLT"
     gene            408..1205
                     /locus_tag="AC162G_01795"
     CDS             408..1205
                     /locus_tag="AC162G_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HIR5074578.1"
                     /translation="MSLPLRLLAGFALSTLIGYAGYGRHALTRSGWLGAVITGTAIFG
                     FGGLAPGLLLIAFFVSSSLLTRYKEGAKAEVAESFAKGGPRDLGQTLANGGVASVVAV
                     LYSIAGEPPWLFAAFVGAMAEANADTWATELGVLSAHRPRLITNGRVVAPGASGGVTW
                     LGTSAALGGAALIGGLAAVFYGDWRLLPLGAIAGLLGSMLDSLLGATVQGIYYSERRG
                     KETEKPIERDGTPNLRVRGWTWMNNDVVNLLGTLAGSLIGLLAYPIR"
     gene            1211..1981
                     /locus_tag="AC162G_01800"
     CDS             1211..1981
                     /locus_tag="AC162G_01800"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR5074579.1"
                     /translation="MKHLLVLFRAILIALAGLCFGCTPDPVSQVIDLVPVPLPSPIPA
                     DVFDFPLDPTRFGPYVHGVTGPLNVDTRFGVQNPGLGSAGKCFVDRNGDRVPFDKLYH
                     AGEDWFALDARGQVSPGLAAGAPVVAVAHGVVSWTQPAGGDGDIIVIEHLLRDGARVW
                     SAYWHLDRVAVVRGQIVRRGDLVGRVLDRGPNSHLHWEIRDWADGSNLFPPDSAGGRG
                     TCNGRAPALGYTWDDRPSRAAPHAWGYLDPAQFVRDHR"
     gene            2113..2412
                     /locus_tag="AC162G_01805"
     CDS             2113..2412
                     /locus_tag="AC162G_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074580.1"
                     /translation="MQKHALVVHPDRGTGILLESWLRKEGYLAQSVRRGSDAVLLARR
                     APVDLIVLDRQAPDSEFSDVILALATDPKSACIPIAFANGNSQDLPIVGTSRLVH"
     gene            complement(2585..3736)
                     /locus_tag="AC162G_01810"
     CDS             complement(2585..3736)
                     /locus_tag="AC162G_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR5074581.1"
                     /translation="MAKKKETAKKPAPKAARRPDASVGTSKPAARAGPGRGLNIDLRY
                     ITDFLVNLLKIPSPTGYTEDAVGYVREVMADLKLDTRLNNKGALVASWKGKASNRPRA
                     LTAHVDTLGAMVKEIDATGRLSLTRIGGVSWTSVETEGCTVITAAGKKIRGAIMPRRA
                     SVHIHGRDGIESARRDDENMWVRLDARTSSNEETRKLGVEVGDFVVFDPRVEVTDTGF
                     IRSRHLDDKAGIACIVGAVKALLDAGQQPSQRLTILISNYEEVGHGASTGIPADVKEL
                     LTVDMAAVGPGQNSDEFSVGICIKDSGGPYHIEMRRKLVALAENAGVPYKLDVYPYYG
                     SDGEAAWRAGADMTVGLVGPGVEASHSYERTHKESLAATAQLIAEFMLS"
     misc_binding    3859..4110
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4328..4495
                     /locus_tag="AC162G_01815"
     CDS             4328..4495
                     /locus_tag="AC162G_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074582.1"
                     /translation="MSAQLDSPVSVKWIVAPEIEDEWQHAGCCVALRPDDPQPPVADD
                     EEDWWPGRCIV"
     gene            4602..>4857
                     /locus_tag="AC162G_01820"
     CDS             4602..>4857
                     /locus_tag="AC162G_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR5074583.1"
                     /translation="MSERYEDSHLYKVRHSAAHIMAQAVMEMFPPGEARIAIGPPIEN
                     GFYYDFDLPRSLTPEDLEAIEKRMREIVAEDHPFVRKEVSA"
BASE COUNT          832 a         1551 c         1539 g          935 t
ORIGIN      
        1 atccggcgag aaaaccccgc cgacaaatac ctcgtccagg cgcgcgtgac ccctcggcga
       61 accgaggccg ggccggcctg gtttcgcgtc ctgttcgcgt tagacgaagt gctgcctttc
      121 tggtggaacg ttcagacgca cgaatatcga cccgtgacgg ccgaacggga agcgtgtttc
      181 ggtctctctg cgctgcgcga cattgcccgg acgatcgccc gcatcagccg gctcgacatc
      241 ttctcgaccg agatcgccca gcgcgccggc gacggtgtgt tcgtgagcat cgactacgtc
      301 aacgatccga tcgatctgcg cctgcaatcc aaagcgccgg acggcgtgcc tgacccagcc
      361 gtgcgcctca tcgccgagcg cattgccggc ctggcggatt ctctcacatg agccttccgc
      421 ttcgtctcct cgccggcttc gcgctgagca cgttgatcgg ctacgccgga tatgggcgcc
      481 atgcactgac acgatccgga tggctgggcg cggtgatcac cggcacggcg atcttcggtt
      541 tcggcgggct ggcgccgggc ctgctcctga tcgcgttctt cgtctcgtcc tcactgctca
      601 cccggtacaa agagggcgcc aaggccgagg tcgccgagtc gtttgccaag ggcggcccgc
      661 gcgacctggg tcagacgctg gccaacggcg gcgtcgcatc cgtcgttgca gttctgtata
      721 gcattgcagg agagccgccc tggctgttcg cggcctttgt cggcgcgatg gccgaagcca
      781 acgccgatac ctgggcaacc gagctgggcg tgctgtcggc gcacaggccg cgcctgatca
      841 cgaacggccg cgtcgtcgcg cccggcgcgt cgggcggcgt gacgtggctg ggaacttcgg
      901 cggcgctggg cggcgcagcg ctgatcggcg ggctggcggc cgtgttttac ggcgactggc
      961 ggctgctgcc cctcggcgcg atcgccgggc tgctcggctc aatgctcgac agcctgctcg
     1021 gcgcgacggt ccagggcatt tactactcgg aaaggcgcgg caaggagacc gagaagccga
     1081 tcgagcgcga tggcacaccc aatctgcgcg tgcgcggctg gacgtggatg aacaacgacg
     1141 tggtcaactt gctggggacg ctggccggct cgctgatcgg actgctggcc tacccaattc
     1201 gctgactatt atgaaacacc tcttggttct gttccgagcg attctgatcg ccctggccgg
     1261 tttgtgcttc gggtgtaccc ctgatccggt cagccaggta atcgatcttg tgcccgtccc
     1321 gcttccttcc ccgatccccg ccgacgtctt cgacttcccc ctcgatccaa cccgcttcgg
     1381 tccgtacgtg cacggcgtca caggcccgct gaacgtcgat acgcgtttcg gcgtgcagaa
     1441 tcccgggctg ggcagcgcgg gcaagtgctt cgtcgatcgg aatggcgacc gcgtcccgtt
     1501 cgacaagctg tatcacgcgg gggaggactg gtttgcgctc gacgcgcggg ggcaggtgtc
     1561 gccggggctg gcagccggcg cgccggtcgt ggccgtcgcg catggggttg tctcgtggac
     1621 gcagccggcc ggcggcgacg gcgacatcat cgtgatcgag catctcctcc gggatggcgc
     1681 gcgtgtgtgg tcggcgtact ggcatctgga tcgtgtcgcc gtcgttcgcg gccagatagt
     1741 gcggcgaggc gatctcgtcg gccgcgtgct cgatcgcggg ccgaactcgc acctgcactg
     1801 ggaaatccgt gattgggccg acggatcgaa tctatttccg cccgactcgg ccgggggacg
     1861 aggcacgtgc aacggccgcg cgccggcgtt gggttacact tgggacgacc gcccgtcgcg
     1921 cgccgcgcca cacgcctggg ggtatctcga tccggcgcaa ttcgttcgag accatcgcta
     1981 aatatcacaa cgctacgcaa actcaatatc cgcccactcc aaacattcct gaatcccatc
     2041 cggcatatac tcactgaagt ttagagttgg ctcccgctga attgacgaat tcagcggaaa
     2101 agcggggtcg atatgcaaaa gcatgcgttg gtcgtgcatc cggaccgggg aacgggcatc
     2161 ttattggaaa gttggctgcg caaggaaggc tacctggcgc agtcggttcg ccgaggctct
     2221 gacgcggtgc tgctcgcccg ccgcgcgccg gtcgatttga tcgtcctcga ccggcaggcg
     2281 cccgattcgg agttctcgga tgtcattctc gcgctggcga ccgatccgaa atccgcatgc
     2341 atcccgatcg cgttcgcgaa cggcaactcg caggatttgc ctatcgtggg caccagccgc
     2401 ctcgtgcatt agatcaagtt cctcctaaag cctcgactcc ggcgtaagcc aaagcggaat
     2461 ccgctctagt ccggttcaga tgcggcaggc tcaagcctgc acttcagttt tcggataggc
     2521 tctaaacaat cgagccgcaa gcgaagccgc ttgcggctcg aaacggatct ctattcgcct
     2581 cggatcacga gagcatgaat tcggcaatga gctgcgccgt ggcggcaagc gattccttgt
     2641 gcgtgcgctc gtacgaatgg ctggcctcga cgcccgggcc gaccagcccg accgtcatgt
     2701 cggcgcccgc acgccaggct gcctcgccgt ccgatccata gtacgggtag acgtcgagct
     2761 tgtacgggac gcctgcattt tcggccaacg cgaccagctt gcgccgcatt tcgatgtggt
     2821 acggcccgcc agagtccttg atgcaaatgc ctaccgagaa ctcgtcgctg ttttggcccg
     2881 ggccgacggc cgccatgtcg accgtgagca gctccttcac gtcggcaggg atgccggtcg
     2941 acgcgccgtg gccgacctct tcgtaattgc tgatcaagat cgtaagtcgc tggctcggct
     3001 gctggcccgc gtcgagcagc gccttgaccg cgccgacgat gcaggcaata ccggccttgt
     3061 cgtccaggtg ccgcgagcga atgaaacccg tgtcggtgac ctcgacgcgc ggatcgaaca
     3121 cgacgaaatc gcccacctcg acgcccagct tgcgcgtctc ctcgttcgac gaggtgcgcg
     3181 cgtcgaggcg cacccacatg ttctcgtcgt cgcgcctggc gctctcgatc ccgtcgcggc
     3241 cgtggatgtg gaccgacgcc cggcgcggca tgattgcgcc gcgaatcttc tttccggcgg
     3301 ccgtgatcac agtgcagccc tcggtctcga cggacgtcca cgataccccg ccgatccggg
     3361 tcaacgagag tcgccccgtc gcatcgattt ccttgaccat cgcgcccagc gtatcgacgt
     3421 gcgccgtcag cgcgcgcggc cggttcgacg ctttgccttt ccaggaggcg acgagagcgc
     3481 ccttattatt caggcgggtg tccagcttca aatcggccat cacctcgcgc acgtagccga
     3541 cggcatcctc ggtgtagccg gtcgggctgg ggattttcag gagattgacc agaaaatccg
     3601 ttatgtatcg caggtcgatg ttgagaccgc gcccagggcc ggctcgagcc gcaggtttgc
     3661 ttgttccaac cgatgcatct ggccttctgg ctgccttggg agccggtttt ttggcagttt
     3721 cctttttctt ggccatggtc atcctctcga cataagtgtt cgaccacgct catctacagt
     3781 aagttaaatg cattatagca caagccattc ggttgacaat ttgatgcaat ccatttataa
     3841 tgtccgcgtc tttgaaaatt gaaagcgtcg agcgggacga gtaaccggcg cgagcgcgcc
     3901 agggagtggg ggtcgaagac tgagagcctc caccgcagcc ctcccggtga ataccccccg
     3961 cgagcgggcg gctgaacgca gaatccaagt aagtcgcccc gggccggccc gttatagcca
     4021 agagtgcggc aacggatata caacggataa acggatacgg atccgctgcc gaaattgggt
     4081 ggaaccgcga gagcgcctct cgtcccatat ggatgagagg cgcttatgtt tggcagcgaa
     4141 aacgctcgcg ccgatgggac gcacgttctg tcgtgaaggg aggtgatgcc gaatgaccga
     4201 agacggcgct ggcactcgca ggaggacgta tcgtatccgc tacgggatga ggcatcgcct
     4261 gtcccgctcg ccggcgaggt aacccgcact gcgcggttgc ctccgccgag acggtttgga
     4321 ggcaaccatg agcgctcaac tcgattcgcc tgtttccgtc aagtggatcg tcgcgccgga
     4381 gatcgaagac gaatggcagc acgccggctg ctgcgtcgcc ctgcggcccg acgacccgca
     4441 gccgccggtc gccgacgacg aagaggattg gtggccgggt cgctgcatcg tgtaagatgc
     4501 cgtgactgga ttatgattcc ttcagttcct tgagttccct tagcgccctt gcctgcttaa
     4561 ggaactcaag ggactcaagg agctgaagaa ggagtttcgc catgtccgag cgatacgaag
     4621 actctcatct gtataaggtc cgtcactccg ccgcgcacat catggcgcag gcggtgatgg
     4681 agatgttccc gcccggcgag gccaggatcg ctatcggccc gccgatcgag aacggcttct
     4741 actacgactt cgatctgccg cgcagcctga cgccggaaga cctggaagcg atcgagaaac
     4801 gcatgcgcga gatcgtcgcc gaggatcacc cgttcgtgcg caaggaagtc tcggcgg
//
LOCUS       DBNHXB010000047         3013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3532786, whole genome shotgun sequence.
ACCESSION   DBNHXB010000047 DBNHXB010000000
VERSION     DBNHXB010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3532786"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            594..669
                     /locus_tag="AC162G_01825"
     tRNA            594..669
                     /locus_tag="AC162G_01825"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:627..629,aa:Arg,seq:tcg)
     gene            complement(812..1222)
                     /locus_tag="AC162G_01830"
     CDS             complement(812..1222)
                     /locus_tag="AC162G_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HIR5074584.1"
                     /translation="MRAVVDTNIFIRALIRPQGSVGPIIPRLAQNEYTLVYSIPLRNE
                     LIAKLALPRIRQKYHVSDTQVEALLDLIALRGDLVTPTRTVRVCRDPKDDMLIEAALA
                     GAAKYVVTGDEDLLVSRRFETMRFVTPRTFLTEF"
     gene            complement(1222..1518)
                     /locus_tag="AC162G_01835"
     CDS             complement(1222..1518)
                     /locus_tag="AC162G_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014643.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR5074585.1"
                     /translation="MNKIIGVTELQRKFRSIFDEVVKRQTPYILTRGSRPEAVLISYE
                     QYLRFIRADEAGVLKRIDDALAYMARVNAQYSDEEVEADVIEATKTVRSRKSKA"
     gene            complement(1819..>3013)
                     /locus_tag="AC162G_01840"
     CDS             complement(1819..>3013)
                     /locus_tag="AC162G_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5074586.1"
                     /translation="HGRVLAALISQFGDFTLAEDALQDALVNALERWEIDGVPRNPGA
                     WLLTVARRRAIDRLRRAATLARKAAILDSLATPDEPEMDDAIPDDRLKLMFTCCHPSL
                     SLEAQVALTLHTLGGLSTPEIARAFLVPVPAMAKRLARARAKIRDAGIPYRVPPPDLL
                     PQRLDALLAVIYLIFNEGYCATSGDTLTRHELCGEAIRLCRVLVALLPALRSAEARGL
                     LALMLLHDARRETRLNAAGELVLLDEQDRARWDQAKIQEGIAVLDEALALHDPGPYQV
                     QAAISALHAEAPAAEATDWRQIAALYGTLATMTPSVVVEVNRAVAAAMAWGAQAGLQM
                     LLRLAGQADGYYPSHAARADLLRRTNQREAAADAYARALALCGNSAERAYLQRRLDEM
                     LNPAV"
BASE COUNT          573 a          921 c          979 g          540 t
ORIGIN      
        1 gcaatgcccc gtccagcgca tagccggcga cggcctggcc accggcccat gccagcccgg
       61 tcaggcgaaa cacgttcggc tgtcgattag gctcgacgac gaaaagcagc tcggacagat
      121 ccagtgacga cgaattaacg taggtagcca cctgctccac ggacaggcgg tgcgacgagt
      181 agtcaaagtg gaccgacagg gcatagcggg tgcgcgcgga agaggatgag gaggcgagag
      241 gcgttgattg agtggctgaa gcaggcgcag gcggtgatcc ggttgcggtg accgcgaccc
      301 cggcgccgga tgggatggcc tcggctgcgc gggatgttgt ggccggcgag tggacaggga
      361 tcgcgtcgca ggacgcgaga accaaaccga taagcgcaac gagtgctgcg cgaacggcga
      421 tgtcaatccg catcacacgg cattttcgcg agtcgagtat catgtacggc tacgtgatcg
      481 cggcgagacc catcctggcc gccggctgtg aaaggtatgg atttgtgtcc aattaccaat
      541 taccagaatt agtttgacgg gtcaatacgc gtgtgctaga atgaccgcag ttggcccccg
      601 tagctcaacg gatagagcga ctgacttcgg atcagtaggt tgtgggttcg attcctgccg
      661 ggggcgccaa tgtatgaggc atggtgattc gtcagaccgg acgtctggcg gtatacaccg
      721 tgcctctctt ctttggtgaa ggaattggtg aaggaatcaa gagtggaagt cgtaactgtc
      781 caggtccttc tcaatggtgg cgatacccat ctcagaattc cgtaagaaag gtgcgcggcg
      841 tgacgaagcg catcgtctcg aacctcctcg acacaagcaa atcctcgtcg cccgtgacga
      901 cgtatttcgc cgcaccggcc agcgcggcct caatcagcat atcatccttc ggatcgcggc
      961 aaaccctcac cgttcgggtt ggcgtaacca agtcgccgcg cagggcaatc aggtccaaca
     1021 gggcttcgac ctgggtatcg ctgacgtgat atttctggcg aatacggggc agcgccagct
     1081 tggcgatgag ctcgttgcgc agaggtatgg aatacaccag ggtgtattcg ttttgggcca
     1141 ggcgtggaat gattgggccg actgaaccct gtggccgtat gagcgcgcgg atgaagatgt
     1201 tcgtgtcgac gacggcacgc atcaggcctt cgatttccgc gagcggacgg tcttggtggc
     1261 ttcgattaca tccgcctcaa cttcctcatc agagtattga gcattgactc gagccatgta
     1321 cgccagcgcg tcgtcaatgc gcttgagcac gcccgcttcg tccgcgcgga tgaagcggag
     1381 gtattgctcg taggaaataa gcaccgcctc gggccggctg ccgcgcgtga gaatatacgg
     1441 ggtctggcgc ttgacgacct cgtcgaagat ggagcggaac ttacgctgca gctcggtcac
     1501 gccgatgatc ttgttcatag aattcccctg gttcgagtac acaccttgct atgtattcta
     1561 gtctgttcgc gggaaaagtc aacccctcaa gtttcttctc cgccacatac gccgccgttt
     1621 cgaccatcgt cgtgtccagc acaccgtagc ccaggccggc gacggcgtga atatgtgtct
     1681 tgggaatcag tatctcctcg cgccaccggc ggaacgaggt gaggaagaaa cgtcgtgacg
     1741 gaggaacgca gcgtaaccaa gttaaggccg gctacgctgg aaggcaggct gaggaaaagt
     1801 cagtcttagc ttctttaatc acactgcggg attgagcatc tcgtcaagcc gccgctgaag
     1861 ataagcgcgc tcggcgctgt tgccgcacag ggcgagcgct cgcgcgtagg catcggcggc
     1921 cgcctcgcgc tggttcgtgc gacgcagcag gtcggcgcgc gccgcgtgag agggataata
     1981 gccgtccgcc tggccggcga ggcgcagcag catctgcaaa cccgcctgcg cgccccaggc
     2041 catcgccgcc gccaccgcgc ggttcacttc gaccaccacc gacggcgtca tcgtcgccag
     2101 cgtgccatag agcgcggcga tctgacgcca gtcggtcgcc tccgccgccg gcgcttcggc
     2161 gtgcagcgcg ctgatcgccg cctgcacctg atagggtccc ggatcgtgaa gggcgagcgc
     2221 ctcgtccaga accgcaatcc cctcttggat tttggcctga tcccagcgtg cccgatcctg
     2281 ttcgtcgagc aggacgagtt cgcccgccgc attaagccgc gtctcgcgcc gcgcatcgtg
     2341 cagcagcatc agcgccagca ggccgcgcgc ctcggcgctc cgcagcgcgg gcagcagcgc
     2401 gaccagcacg cggcacagac ggatcgcttc cccgcacagc tcgtgccggg tgagcgtatc
     2461 gccgctggta gcgcagtaac cctcgttgaa gatcaggtag atcaccgcca gcagcgcgtc
     2521 gagtcgttgc gggagcaaat cggggggcgg cacgcgatag gggatgcccg cgtcgcggat
     2581 ttttgcccgc gcccgcgcca gccgctttgc catcgccggc acaggcacga gaaacgcccg
     2641 cgcgatctcc ggcgtagaca gcccgcccag cgtgtgcagc gtcagcgcga cctgcgcttc
     2701 cagcgacagc gaaggatggc agcaggtgaa catcagtttc aggcgatcat cgggaatcgc
     2761 gtcgtccatc tcgggttcgt ccggggtggc gagggagtcg aggatggccg ctttgcgcgc
     2821 cagcgtcgcc gcgcggcgca ggcggtcgat ggcgcggcgc ctggcgacgg tcaacaacca
     2881 ggcgccgggg ttgcgcggaa cgccgtcgat ctcccagcgt tccagcgcat tgaccagcgc
     2941 atcttgcagc gcgtcctccg ccagcgtgaa atccccgaac tggctgatga gcgccgccag
     3001 gacgcgcccg tgc
//
LOCUS       DBNHXB010000048         8340 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1016339, whole genome shotgun sequence.
ACCESSION   DBNHXB010000048 DBNHXB010000000
VERSION     DBNHXB010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8340)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8340
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1016339"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="AC162G_01845"
     CDS             <1..212
                     /locus_tag="AC162G_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074587.1"
                     /translation="EVSALVRDEAQVDHVEFRVDGRTWASLSAAPFRVWWSLAEGKHR
                     LVAIAVDRQGRRSVSDPVTVVVEEQ"
     gene            234..6116
                     /locus_tag="AC162G_01850"
     CDS             234..6116
                     /locus_tag="AC162G_01850"
                     /inference="COORDINATES: protein motif:HMM:NF019323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5074588.1"
                     /translation="MSYRTARIVRIGTALAVTALVILTLSRCATASSVRVIEWSPGVI
                     DDAPINAPISLTFDQRMDADSIAAAFHIRPAVSGRFVATHYEPPSTLQSPLVSAAAHS
                     PLASQDGPFSVIVFLPDRPLEKATQYQIAVDASVRSQSGQTLREPFTAAFTTTGQLHV
                     GFYPAIGKTNVPTGTAIGLSFNAPFSATGQVPIFITPPVTGAGRWLSSRRYEFEPSAL
                     QPATRYTVRLPAGLRGPGGEKLLSDAVTTFTTTLPAVADHAPDGYLVAPSTAVTLTFN
                     LPVDPASVESRFAMRGMDRQTISGTLAWVDDQTLVFTPADRLLEGETYSATVANGIRT
                     PLGDLPSAQPFTYDFRIAPKPELVSSSPSDGDLKAAIGDRVTLRFNVPMDSVSVERAL
                     RIEPAVAEGEPRFEWDTSHTRLDIVYNIDPTALYTLALGDEARDFLNRPLTGNRSIVF
                     QAGPQKAVTWLVGPRGYWNNVYGTYNPNPQVRQYTQFRNVEQITYRLSSITRKDFLSA
                     FRPNWYWDENKILPASTPIFTWTQAVSAPLNALAFATTDVTLPDGSPLPSGAYLLETD
                     GMPGHSEDWRVLVVSPLNLALKRSERQLFVWATDLRTGQPVSGLSLTVYDQDKHLVTS
                     GATNQVGVFITDQLPECFDRWECAYGWRPLYVVAEAESGEWGFVASEWDEGIATRDFR
                     MPYYYGEPSHTVFLYTDRPIYRPGQTVYFKGVVRADDDGRYRLPAFSRVPVVIQDAQG
                     NEIFREELPLTDVGTLYGEFTLSAEAALGSYSVQVRHAEDEPPAYGNFRVAEYRKPEF
                     KVSVTPNTTTPLHGEAVTVVVQADYYFGAPLPNAAVEWRLSSEDYLFSLPGAWYSFGD
                     VDEYYWLWGEESDSGQNIYANGTGRTDVNGTFTFTVPIDLSQAKRGQVLTFEADVVDA
                     NHQVTSARGYAVAHKSALYVGARPRWYVTRPNNSLEIQLVAADPGKNRLPGMLLTVEL
                     YDRVWKSVRVRDSYGTYHWNSTSSDTLISTQVVTTGVNGEVGAVVVPPRGGSYRVVAR
                     GKDVHGVESVASTFFWTWGGEYVNWGIQNDDRISVIADKRTYQPGETAHLLITAPFAD
                     STALVSVERGGVLRYWTLPIKSTSALIDVPIHADYAPNAFVSVILIKGQAPDFPAADF
                     KIGYAALNVELTEQRLNVELIPDKTRYAPRDTALYIVRVTDHLGQPVDAEVSLSLIDA
                     AVLALVGDEDRDILTAFYRQRGLGVHNSLTLVTSVDRLTAILDKKAKGGGGGGEVTER
                     DLFADTAFWRAAVRTGPAGETTVSVPLPDNLTTWRMRAKAVSTDTQLGQTEVDVIATQ
                     DFVLRPVLPRFFTVGDQAQVGAIFHNYTPITETLNVSLDFAGRDDFTPQQITIGPNGS
                     APAYWNVSVPRTLSVTVRLQAVTADGRGDAVRLPLPVNAFFDRVSYSNYRSITGTQAI
                     PIALPPDVDRNLDELVIEVEPTLAASIDSGLEYLIGFPYGCVEQTMSSFLPDVVVMRL
                     IQQVGIDTRPDFREQLSDMIESGMQRLYGYQHNDGGWGWWKDDDSNPAITAYVLYGLH
                     QMELGGRPIDAKVRDAAVSYLLNWLQRTSVDAPIGPTHGADAVSSGANVRAYALYVLA
                     ELGHGNPGLTGRLYEQRDKLDPYGKAYLALALYLTNDRAIDARIETLVQELRDAAKKE
                     GNTLYWEDRQRDYWGMSTSVRSTAIAIDALVRLFIEDPTIDPAARWLLMQRQEGRWNT
                     TQETSLSLAALTDYVVSTFEREGNFTYTVRVNGQVVATESVTPQTLGRHGKWVVPLAN
                     LGAGDPVVEIARGEGPGAPLRVSVSLRHYRAGDDIAPIDNHGVHVTRTYSKPLDQLRP
                     GDVVSVTLNVRFDDGGTYVLVEDPLPAGWEPIDASLATTSQQFQGRSGDWVWTHVELR
                     DDRVALFATWIPGGHTYTYTYRARVTTAGRFYALPMQAYAMYSPDIVGRTPGTVMQVS
                     ER"
     gene            complement(6135..6278)
                     /locus_tag="AC162G_01855"
                     /pseudo
     CDS             complement(6135..6278)
                     /locus_tag="AC162G_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6826..>8340
                     /locus_tag="AC162G_01860"
     CDS             6826..>8340
                     /locus_tag="AC162G_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074589.1"
                     /translation="MSTRLHTVFAVLVVWGVLAAQPAAVTMTVEPYFGGHFKFGEWLP
                     LHVSLANDGPPVQAEVRADTTEAGGQTTYSTPIELPTGARKRLTLYVQPPSFAQAVRV
                     RLMDGTRELASRSVQVTVERNVNYVVGIVALRTEAFAVLAGLTLNPSSTDQNAQIVGI
                     VGGQQQPRLVTRISMPLADIPDRFEGLRALDALIISGVDTSELTSDQGRALQAWVEQG
                     GRLILGGGAGAARTLAGLPQALVENWRPTSGPAEISSLSAFEEFTGQAVRVPGPFSVT
                     WPETGRALLEQGGRALLAEERLGEGRVNYAALDLAGSPFDAWAGATRFWEKLLTPGSA
                     YPLNVPPDVSPRVMRANSIYFALQNLPVLELPSIDWLTGLLGVYIALVGPVNYLVLRR
                     MRKLAWGWITILALTLLFSAGAFALGFNIRGSDVIVNKISMLTFDTRGTAASMQSYVG
                     VFSPERSAYTLSFPGRALVAPIAFEGSPWGPWSPGGPAVEYARVEIVQGEPAQAR"
BASE COUNT         1490 a         2727 c         2618 g         1505 t
ORIGIN      
        1 tcgaagtctc cgctctcgtt cgcgacgaag cacaagtcga tcacgtcgag ttccgggtgg
       61 atggcaggac ctgggcgagt ctcagcgccg cgccgtttcg cgtctggtgg tcactggccg
      121 aaggtaaaca tcgcctcgtc gccatcgccg tcgatcggca gggccggcgc tcggtcagcg
      181 atccggtcac cgtcgttgtg gaagagcagt gagtctcatt gaggagtgct gcaatgtcct
      241 accgaactgc acgcatcgtc cgcatcggca cagccctcgc ggtcaccgcg ctggttatac
      301 tgacgttaag ccgctgcgcc actgcgtcca gtgtgcgcgt gattgaatgg tcgccgggcg
      361 tgattgacga cgcgccgatt aatgcgccca tctcgctcac attcgatcag cgcatggatg
      421 cagactcgat cgcggcggcg tttcacatca ggccagccgt ttcgggacgc ttcgtcgcca
      481 cacattacga accgccgtcg acgctccagt cgccgctcgt ctcggcggcc gctcattctc
      541 cgctagcgag ccaagatgga ccatttagcg tgatcgtatt cctaccggat cggccattgg
      601 agaaagcgac gcagtatcag attgcggtcg atgcctcggt gcgcagccag tcggggcaga
      661 cattgcggga acccttcact gccgcgttca cgacgaccgg ccaactgcat gtcgggtttt
      721 acccggccat tgggaaaacc aacgtcccta ccggcaccgc catcggcctg tcattcaacg
      781 cgccattttc cgccactggt caggtgccca tcttcatcac tccaccggtt accggcgcgg
      841 gccggtggct gagcagccgg cgctacgagt tcgagccttc cgcgttgcag cccgccacgc
      901 gttacaccgt gcgcctgccc gccggtctga gaggccccgg tggtgaaaag ctgctcagcg
      961 acgccgtgac gaccttcacc acaacactgc cggccgtagc cgaccacgcg cccgacggct
     1021 acctcgtcgc gccgtcgacc gccgtcaccc tcactttcaa tctcccggtg gatcccgcga
     1081 gcgtcgagtc gcgcttcgcg atgcgcggca tggacaggca gaccatctcc ggaacgctcg
     1141 cctgggtcga cgaccagaca ctcgtcttca caccggcgga caggctgctg gaaggcgaga
     1201 cctactcggc cacggtagcc aatggcatcc gtacgcctct gggcgacctg ccttccgcgc
     1261 agccgttcac ttatgatttc cgcatcgcgc cgaagcccga gctggtcagc tcgagcccga
     1321 gcgacggcga cttgaaagcc gctatcggcg atcgcgtgac gctgcgcttc aacgtgccga
     1381 tggacagcgt ctcggtcgag cgcgcgctgc gcatcgagcc ggctgtcgcc gagggcgagc
     1441 cgcgcttcga atgggatacc agccacaccc gtctcgacat cgtctacaac atcgacccga
     1501 ctgctctata caccctcgcg ctaggcgacg aagccagaga cttcctgaac cggccgttga
     1561 cgggcaatcg cagcattgtc tttcaggccg ggccgcagaa agctgtgacg tggctggtgg
     1621 ggccgcgcgg ctattggaac aatgtctacg gcacgtacaa cccgaacccc caggtgcggc
     1681 agtacacgca gtttcgcaac gtcgagcaga tcacgtatcg cctttcatcg attactcgca
     1741 aggatttcct gtcagccttc aggccgaact ggtattggga cgagaacaag atccttccgg
     1801 ccagcacgcc catcttcacc tggacacaag cggtcagcgc gccgctcaac gcgctggctt
     1861 tcgccacgac ggacgtcacg ctgcctgatg gatcgccgct gccgtccggc gcatatttgc
     1921 ttgaaaccga tggcatgccc ggccattccg aagactggcg cgtactggtt gtgtcgccgc
     1981 tcaatttggc gctcaagcgc agcgagcgac agctctttgt gtgggccacc gatctgcgaa
     2041 cgggacaacc cgtgagcggc ctttcgttga ccgtgtacga ccaggacaag cacctcgtta
     2101 cgtccggcgc gaccaaccag gtgggcgtat tcattaccga tcaactgccg gagtgcttcg
     2161 accggtggga gtgcgcctac ggctggcgcc cgctgtacgt cgtcgccgag gccgagtcgg
     2221 gcgagtgggg tttcgtggca agcgaatggg atgagggcat cgccacgcgc gacttccgca
     2281 tgccgtacta ctacggcgag ccgtcgcaca ccgtctttct ctacaccgat cgccccatct
     2341 atcgtcccgg ccagacggtg tacttcaaag gcgtcgtccg cgccgacgac gacgggcgct
     2401 accggctgcc cgccttcagc cgcgtgcccg tcgttatcca ggacgcgcag ggcaacgaga
     2461 tctttcgcga agagctgccg ttgaccgacg tcggcacgct gtacggcgag ttcacattga
     2521 gcgcggaagc cgcgttgggg tcgtattcgg tgcaggtccg ccatgccgag gacgagccgc
     2581 cggcctacgg caacttccgc gtcgccgagt atcgcaagcc ggagttcaag gtgagcgtga
     2641 cgccgaacac gaccacgccg ctgcatggcg aggccgtcac cgtcgtcgtg caggccgatt
     2701 actacttcgg cgcgccgctg cccaacgcag ccgtcgagtg gcgtctgtcg agcgaggact
     2761 atctcttctc gctgcctggt gcctggtaca gcttcggcga cgtcgacgaa tactactggt
     2821 tatggggtga ggaatccgat agcgggcaaa acatctatgc caatggaacc ggccggaccg
     2881 acgtcaacgg aacgttcacc ttcaccgtgc ccatcgacct gtcacaggcc aaacgcggtc
     2941 aggtgctcac ctttgaagcc gacgttgtcg acgccaatca ccaggtcacc agtgcgcgcg
     3001 gctacgccgt ggcgcacaag agcgcgttgt acgtcggcgc gcgcccgcgc tggtacgtca
     3061 ccaggcccaa caactctctc gaaatccagc tcgtcgccgc cgaccctggc aagaacaggc
     3121 tgccgggcat gctgctgacc gtcgagctgt acgatcgcgt gtggaagtct gtccgcgtgc
     3181 gcgacagcta cggcacctac cactggaaca gcacctccag cgatacgctg atcagcacgc
     3241 aggtcgtcac caccggcgtg aatggagaag tgggcgccgt cgtcgtgccg ccgagaggtg
     3301 gctcctatcg cgttgtcgcg cgcgggaagg atgtgcacgg cgtcgagtcg gtcgcctcaa
     3361 ctttcttctg gacgtggggc ggcgagtacg tcaactgggg cattcagaac gacgatcgca
     3421 tcagcgtcat cgccgacaag cgcacgtacc aacccggcga gaccgctcat ctgttgatca
     3481 cggcgccctt cgccgacagc accgcgctgg tcagcgtcga gcgcggcggc gtgctgcgct
     3541 actggacgct gcccattaag agcaccagcg cgctgatcga cgtgccgatc catgccgact
     3601 acgcgcccaa tgcgttcgtc agcgtgattt tgatcaaagg ccaggcaccc gatttccccg
     3661 ccgccgattt caagatcggc tacgcggcgc tcaacgtcga gctgaccgag cagcgactca
     3721 acgtcgagct gattcctgac aagacccgct acgcgccgcg cgacaccgct ctctacatcg
     3781 tgcgcgttac cgatcacctc ggccagccgg tcgacgccga agtgtcgctc agcctgatcg
     3841 acgcggcggt gctggcgctg gtcggcgacg aagaccgcga catcttgacg gccttttatc
     3901 gccagcgcgg gttgggtgtg cacaacagcc tgacgctggt gacgagcgtc gatcgactca
     3961 ccgctatcct cgacaagaaa gccaaaggcg gcggtggcgg cggcgaggtc accgagcgcg
     4021 acctgttcgc cgacactgca ttctggcggg cagccgtccg caccgggccg gcaggcgaga
     4081 cgacggtgag tgtgccgctc cccgacaatt tgacgacgtg gcgcatgcgg gccaaagccg
     4141 tcagcaccga cacgcagctc ggccagacgg aggtcgacgt gatcgccacg caggactttg
     4201 tgctgcggcc ggttctgccg cgtttcttca cggttggcga tcaggcgcag gtcggcgcca
     4261 tcttccacaa ttacacaccc atcaccgaga cgctgaacgt gtccctcgac tttgcggggc
     4321 gagacgactt cacgccgcag cagattacta tcggcccgaa cgggtcggcg cccgcttact
     4381 ggaacgtgag cgtgccgcgc acactgtcgg tcacggttcg gctgcaggcg gtcactgccg
     4441 acgggcgcgg cgatgccgtc aggctgccgc ttcctgtcaa cgctttcttc gatcgggtct
     4501 cgtacagcaa ctaccggtcg atcacgggca cgcaggcgat cccgattgcg ctgccgcctg
     4561 acgtcgaccg gaatctcgac gagctggtga tcgaagtcga gccgacgctg gcggccagca
     4621 tcgacagcgg gctggagtat ctgatcgggt tcccctacgg ctgcgtggag cagacgatga
     4681 gcagcttcct gcccgacgtc gtcgtgatgc gcctgatcca gcaggtcggc atcgacacgc
     4741 ggcccgactt ccgcgagcag ttgagcgaca tgatcgaatc gggcatgcag cggctgtacg
     4801 gctaccagca caacgacggt ggctggggct ggtggaagga cgacgacagc aacccggcga
     4861 ttacggccta cgtactgtac ggtctgcatc aaatggagtt gggcgggcgg cccatcgatg
     4921 ccaaagtgcg cgacgcggcg gtgagctatc tcctcaactg gctgcagcgc acatcggtcg
     4981 acgcgccgat cgggccgacg cacggcgccg acgcagtgtc gagcggggcg aacgtccgcg
     5041 cctacgcgtt gtacgtgctg gccgagctgg gacacggcaa ccccggcctc accggccggt
     5101 tgtacgagca gcgcgacaag ctcgatccat atggcaaggc ctacctggcg ctggcgctgt
     5161 acctgacgaa cgatcgcgcc attgacgcgc gcatcgagac gttggtgcag gagctgcgcg
     5221 acgcggcgaa gaaggaagga aacacgctct actgggagga tcggcagcgc gattactggg
     5281 gcatgagcac cagcgtgcgc tcgacggcca tcgccatcga cgccctggtc cggctgttca
     5341 tcgaagaccc caccatcgat ccggcggcgc gctggctgtt gatgcagcgg caggaaggtc
     5401 gctggaacac cacacaggag acgagcctca gccttgctgc gctgaccgat tacgtggtca
     5461 gcaccttcga gcgcgagggc aactttacct acacggtgcg cgtcaacggc caggttgtgg
     5521 cgacggagag cgtcacgccg cagacgctcg ggcggcatgg caagtgggtc gtgccgctgg
     5581 ctaatctcgg cgcaggtgac cccgtcgtcg agatcgcgcg cggcgaaggt cccggcgcgc
     5641 cgctgcgggt gtcggtcagc ctgcgccatt accgcgccgg ggacgacatc gcgccgatcg
     5701 acaatcatgg cgtccacgtc acccggacct acagcaagcc gctggaccag ctccggccgg
     5761 gcgacgtcgt cagcgtcacg ctcaacgtcc gcttcgacga cggcggcacc tacgtgctag
     5821 tcgaagatcc gttgcctgcc ggatgggagc cgatcgacgc gagcctggcg acgaccagcc
     5881 agcagttcca ggggcgctcc ggggattggg tgtggacgca cgtcgagctg cgtgacgacc
     5941 gcgtggcgtt gttcgcgacg tggatacccg gcggccacac ctacacctac acctatcgcg
     6001 cgcgggttac gacggccggc cggttctacg cgctgccgat gcaggcctac gccatgtaca
     6061 gcccggacat cgtcgggcgc acgccgggca cggtgatgca ggtgagcgag cgctgaggcg
     6121 cgagaggact ggactcacaa tcgtggagcc accggctcac tctccagcgc cggtatccag
     6181 aacacgcact cgtgtacgcg atagggcggc tcaccgcgag cgaaacgctc catcacctcg
     6241 acgcttagcg cgaactcacg tagggcgagt gaccgtttac cagagaatcc acttctgtcg
     6301 cccgcatcac gccctgcgct tcagactgga tatcccccag gcacatctcg gacgccaagc
     6361 gccaagccag ctgtgctggt cggctcggct gatcacatct ggagtgtgga aggcaacacg
     6421 gttcgaacta acgtcgagat atgctatggc cgaacttggc agacggctac aatcaggggg
     6481 gaattcaaac ctgatataat cctcgtcaca tcgtccatcg tcgcgcttgc ccggtctgtg
     6541 ccggtccgcc tacaagcgac gatccacgtt ggacacggac agtcgtgcac gccttcgcca
     6601 tccggattct gggttcgcta agaagggacc tatttttctg gtggaggaac ttatcatctt
     6661 ccgtcagcgt cttagaatcg cttccgcttg gatttacgac ttggaatcga ggctttagcc
     6721 ggatccgcct gaaggctcga ctccggaata ccgtaaactc aactggaaac cgctataagt
     6781 aggaacttgc cggatttgca ctccctcctc tgaagtggag accctatgtc tacccggctg
     6841 catactgtct ttgcggtgtt ggtcgtctgg ggcgtgctcg ccgcacagcc ggccgccgtg
     6901 acgatgacgg tcgaacccta cttcggcggc catttcaagt ttggtgagtg gttgccgctg
     6961 cacgtcagcc tggcaaacga tggccctccc gtccaggcag aagtccgcgc ggatacgacc
     7021 gaggcgggcg gtcaaaccac ctacagcacc ccgatcgaac tgcctactgg cgcgcgcaag
     7081 cggctgactt tgtacgtgca gcctccctcg ttcgcgcagg ccgtccgcgt gcgattgatg
     7141 gatgggacgc gcgagttagc cagccggtcc gtgcaggtga ccgtcgagcg taacgtcaac
     7201 tatgtggtcg gcatcgtcgc actgcgcacc gaggcgttcg ccgtactggc tgggctgacg
     7261 ctcaatcctt cgtccaccga tcagaatgcc cagatagttg ggatagttgg gggacagcag
     7321 cagccgcgcc tcgtcacgcg gatttcgatg cccctggccg acatccccga tcgttttgag
     7381 gggctgcgcg cactggacgc gctgatcatt tccggcgtgg atacgagcga gctgacctcg
     7441 gatcaagggc gcgccttgca agcctgggta gagcagggcg ggcggctgat attgggcggc
     7501 ggcgcaggcg cggcccgaac actcgccgga ctgccccagg cgctcgtgga gaattggcga
     7561 cccacaagtg gccctgccga gatttcatct ctgagcgcct tcgaggaatt cacgggtcaa
     7621 gcggtgcgtg tcccaggccc cttcagtgtg acatggcctg agaccggtcg cgctctactc
     7681 gaacaaggcg gccgcgcatt gctggccgag gagcgcttgg gcgaaggccg tgtgaactac
     7741 gctgcgctcg acctggccgg cagtcccttt gatgcctggg cgggggccac acgcttttgg
     7801 gaaaaactgc tcacgcccgg ctctgcctat ccgctcaatg tgcccccgga tgtttcacca
     7861 cgtgtgatgc gtgcaaactc gatctacttc gccctgcaga acctgcccgt cctcgaactt
     7921 ccttcgatcg attggctgac cggcctgctg ggggtctaca tcgcgctcgt cggcccggtc
     7981 aattacctgg tgttgcgccg gatgcgaaaa ctggcctggg gttggatcac catcctggcg
     8041 ttgacactgt tgttttcagc cggggcgttc gcgctcggct tcaacatccg aggcagcgat
     8101 gtgatcgtca acaagatttc gatgctcact ttcgatacac gggggacggc cgcgtctatg
     8161 cagtcgtacg tcggcgtatt ctcgccggaa cgcagcgcct acacgctgag cttccccggc
     8221 cgtgcactcg tggcgccgat cgcgttcgaa ggtagcccct ggggcccttg gagtccgggc
     8281 ggtccagcag tcgagtatgc gcgggtggag atcgtgcagg gcgaaccggc tcaggcgcgc
//
LOCUS       DBNHXB010000049         4061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_177895, whole genome shotgun sequence.
ACCESSION   DBNHXB010000049 DBNHXB010000000
VERSION     DBNHXB010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_177895"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1135
                     /locus_tag="AC162G_01865"
     CDS             224..1135
                     /locus_tag="AC162G_01865"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR5074590.1"
                     /translation="MADTFVIVNPASGSGKTGRRWPGLDARIRAEGGEFDVEFTHEPG
                     HAEHLARTAAAGGAKTVIVVGGDGTLNEVVNGLIAGDVPIRDVTIGILPVGTGSDFAR
                     AMGVPRDPLAAAIHLLRRARPQPLDVGRIDCVLGGQSSTRYFVNVAGLGFDGEVADRV
                     NRAGKSGGTLTYQLALLQSLGAYQNKHVRVTIDGHSREGVMNSVILANARYIGGGMFI
                     APNARWDDGRFDVILLGDFGKLEVVANLPRLYRGTHLTHPKVTELHATEVTVEARERM
                     FIQAEGELAGEAPATFRILPGALKILR"
     gene            1440..1841
                     /locus_tag="AC162G_01870"
     CDS             1440..1841
                     /locus_tag="AC162G_01870"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5074591.1"
                     /translation="MSDSTPPGQPATILIVDDEPETRALFRMMLKPYANWRILEAETG
                     QRAIEIAKAERPDVILLDVMMPDLDGFGVCARLRQSLRLYAGKIVMLTVLHTPTSRKA
                     AVAAGADDFWTKPISPQALRSGLARLLGGGA"
     gene            complement(1786..>4061)
                     /locus_tag="AC162G_01875"
     CDS             complement(1786..>4061)
                     /locus_tag="AC162G_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013239.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HIR5074592.1"
                     /translation="DKGYNYWRIQLPAFRSKQRVTYRAHAGQGERRLSSESLSFVVAG
                     WRSMGDVVEYHLASDHLELVCAYGEPTLRPRVVISFRGPYLLHLRLTVENSASLSRSS
                     APAEKQQVSTSLRYRILEETNEHLRIATAGLRVTIHRHPYRLEVAKSDGTPLLYEADP
                     PAWLIGEDGRALHVRQVFISPPDEGFYGFGERYNALDQRGSELDVRVFEQYKNQGRRT
                     YLPVPFFISSRAYGLYLATHRHVVYDLAASRPGHWSLDAEVGPDGSLESYVIVGDPKQ
                     VITAFTDLTGKPALPPAWTFGPWMSGNEWNSQAVVMEQVRKTLEHHIPASVLVIEAWS
                     DESTFYIWNDAHYAPKPAHEPFTYGDFTFPPDGRWPDPKGMIDELHRLGIRVLLWQIP
                     VIKKLDVPHPQQEADEAYALANGYCVREADGQPYRIRPFWFHGGLVLDFTDPTAVQWW
                     LSKRAYLLDELGIDGFKTDGGEHLWGRNLRFADGRRSDELWNLYPNLYVGAYHRFTRD
                     RRNGDALTFSRAGFTGAQAYPCHWAGDENSTWEAFRASILAGLNAGLSGIPFWGWDLA
                     GFSGEIPSAELYLRAAAMAAFCPIMQYHSEYNEHQKPCRDRTPWNIAERTGEPRVIEI
                     YRKFAQLRMQLLPYIEAEAAHGAVTGEPLMRALFLDWPEDPITWKITDQYSFGRALLV
                     APVVEPGITERRIYLPAGEWEDFWSGAQFSGRQWITRSAPLDVIPVFRRRGVTWPGAA
                     KRHRRAGARDRCAALVD"
BASE COUNT          835 a         1243 c         1211 g          772 t
ORIGIN      
        1 cacaccgatt tccccgggga cgtttttagg ctccgaagtt ggcaagtatc ccgctatcga
       61 cctgggcgat tgcgcattca ttttgcacct tgaccgtgcc ggaatacttg cccatccgtg
      121 taatccgcct gccctcgcct gctgtgtgcg ggggtgggaa atccgtgtgt cgggtctatg
      181 acttcagtat ataattgcga cacctctgcg cgagggacac ggcatggccg acacgtttgt
      241 gatcgtcaat ccagcgtcgg gcagcggcaa gaccggcagg cgctggcccg gtctcgacgc
      301 gcgcatacgc gccgaaggcg gggagtttga cgtcgagttc acgcacgagc cgggacatgc
      361 cgagcatctc gcgcgtacgg cggcagcagg cggcgctaaa accgtcatcg tcgtcggcgg
      421 cgatggaact ttgaacgaag tcgtcaacgg cctgatcgcc ggcgatgtgc cgatccgcga
      481 cgtgaccatc gggatactgc ccgtgggtac cggttcggat tttgcccgcg cgatgggcgt
      541 gccccgtgat ccgctcgcgg cggcgatcca cctgcttcgg cgcgcccgtc cgcagccgct
      601 agacgtcggc cgcatcgact gcgtcctcgg cgggcagtcg tcgacgcgct acttcgtcaa
      661 cgtcgccggt ctcggcttcg acggtgaagt cgccgaccgc gtcaaccgcg cgggcaagag
      721 cggaggcaca ctgacctacc agctcgcact gcttcagagc ctgggcgcgt atcagaataa
      781 gcatgtccgg gtcacgatcg acggccactc gcgcgaaggc gtcatgaact cggtaatcct
      841 cgccaatgcg cgctacatcg gcggcggcat gtttatcgcc ccgaacgcgc gatgggacga
      901 cggccgcttc gacgtgatct tgctgggcga tttcggcaag ctggaggtcg tcgccaactt
      961 gccgagactg taccgtggca cgcacctgac ccacccgaag gtcaccgagc tgcacgcgac
     1021 cgaagtgacc gtcgaggcgc gcgagcgcat gttcatccag gccgagggcg agctggccgg
     1081 cgaagcgccc gcgacgttcc gcatcctgcc gggtgcgctg aagatactgc gctgaagaag
     1141 cagaaccccg gtttttgcca tttctcctcg aaactggggc aaatcgcctc acgcatcagg
     1201 ctctattgaa aatgctccca aaatgcatgg caatccgtat gggttgaggc cagaccactc
     1261 atcccagcaa tgaaatgcgc attcgttata cgactgttat gcgcgggcca taactaaaag
     1321 aggggtcgca cgttctgaca agtagccgat gtgcagcgct ctgtacagaa caaggggagg
     1381 cgcgaggaca atctgcttga gcgtgcaccg cgatatctac ttcgcggaaa ggagtgtccg
     1441 tgtccgactc aactccgcct gggcaacctg caacgatctt gatcgtggac gacgaaccgg
     1501 agacacgcgc gctgttccgg atgatgctca agccgtacgc caactggcgc attctggagg
     1561 cggagacggg tcagcgagcc atcgaaatcg ccaaagccga gcggcccgat gtgatcctgc
     1621 tcgacgtcat gatgcctgac ctcgacggct ttggggtgtg cgcccggttg cgccaatcac
     1681 tccgcctgta tgccggcaaa atcgtgatgc tgactgtgct ccacaccccg acgtcacgca
     1741 aggccgccgt cgctgccggg gcggacgatt tctggaccaa gccgatcagt ccacaagcgc
     1801 tgcgcagcgg tctcgcgcgc ctgctcggcg gtggcgctta gccgcgccgg gccatgtgac
     1861 gcctcggcga cgaaagacgg gaatcacgtc cagcggagca gaccgcgtga tccactggcg
     1921 gccggaaaat tgcgctccgc tccagaaatc ctcccactct cccgccggca gatatatgcg
     1981 tcgctctgtg atacctggtt caaccactgg agcgaccagt aacgcacgcc caaagctata
     2041 ctggtcggta atcttccacg tgatcggatc ttcaggccag tccaggaata aggccctcat
     2101 gagcggctcg cctgtcaccg caccgtgcgc cgcctccgct tcaatatagg gcagcaactg
     2161 catgcgcaac tgggcgaact tgcgatagat ctcgatcacc ctcggttcgc cagtgcgttc
     2221 ggcgatgttc cagggcgtgc ggtcgcggca gggcttttgg tgttcgttgt actccgagtg
     2281 atactgcatg attgggcaga aggctgccat agctgcagcc cggagataaa gctcggccga
     2341 aggaatctcg ccactgaacc cggccaggtc ccagccccag aatgggatgc ccgagagtcc
     2401 ggcgttcaac ccagccagga tggaagcgcg gaaggcttcc caggtggaat tctcgtcacc
     2461 ggcccagtga cagggataag cctgggcacc cgtaaatcca gcccggctga atgtgagggc
     2521 atcgccgttg cgcctgtccc gtgtgaagcg atgataagcg cccacgtaca ggttcgggta
     2581 gagattccac aactcgtcac tccggcgacc gtcggcgaag cgcagatttc gtccccacag
     2641 gtgttcgccg ccatcggtct tgaagccatc gattccgagc tcgtccaaca aataagcccg
     2701 cttgctcaac caccactgca cggcggtggg gtcggtaaaa tccaatacca gaccgccgtg
     2761 aaaccagaat gggcggatgc gatagggctg accgtctgcc tcgcgaacgc aatagccatt
     2821 ggccaatgcg taagcttcat cagcctcttg ttgcggatgg ggcacatcca gtttcttgat
     2881 cacgggtatc tgccacaaga gcactcggat acccagacgg tgaagctcgt caatcatgcc
     2941 tttcggatcg ggccagcgcc catcaggtgg aaaggtgaag tcaccgtagg tgaatggttc
     3001 gtgagcgggc ttcggcgcat agtgcgcatc gttccagatg taaaaggtag attcatcact
     3061 ccaggcctca atgaccagga cgctggccgg gatgtggtgc tccagggtct tgcgaacttg
     3121 ctccatcacc acggcctggc tgttccactc gttgccgctc atccaaggcc cgaaggtcca
     3181 ggcaggcggc agcgccggtt tgcccgtcaa atccgtaaag gctgtgatga cctgtttggg
     3241 atcgcccaca atgacgtagg actccaggct gccgtccggt cccacttccg cgtcgagcga
     3301 ccagtgcccc ggccgagagg ctgccagatc atagacgaca tgccgatggg tagcgagata
     3361 caaaccatag gctcgcgatg agatgaaaaa aggaacaggc aggtaggtgc ggcgcccctg
     3421 attcttatac tgctcaaaga ccctcacatc caattcgctg ccgcgctgat ccagggcgtt
     3481 gtatcgttct ccgaagccat agaagccttc gtcaggcggg ctgatgaaga cctgtcgcac
     3541 gtgtaaggcc cggccatcct caccgatcag ccaagctggc gggtctgcct cgtacagaag
     3601 cggagtccca tctgacttgg ctacttctaa ccggtaggga tggcgatgga tagtaacccg
     3661 caagcctgct gtagcgatcc taagatgttc attcgtttcc tccaagatcc gataccgcaa
     3721 cgaggtggac acttgttgct tttcagcagg ggcggatgaa cgtgagagtg atgcactgtt
     3781 ttcaacggtt agccgcaggt gtaacaggta tgggccacgg aaggatatca caacacgagg
     3841 ccgcagagtc ggctcaccgt atgcgcacac caactccaga tgatccgaag ccagatgata
     3901 ctcgacgaca tcgcccattg agcgccatcc agccacgaca aaagaaagac tctccgaact
     3961 cagtcgtcgc tcaccctgac cggcgtgcgc gcgataggtc actcgctgct ttgaacgaaa
     4021 agccggaagt tggatccgcc agtaattgta tcccttatca t
//
LOCUS       DBNHXB010000050         5548 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_681201, whole genome shotgun sequence.
ACCESSION   DBNHXB010000050 DBNHXB010000000
VERSION     DBNHXB010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_681201"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="AC162G_01880"
     CDS             <1..299
                     /locus_tag="AC162G_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR5074593.1"
                     /translation="VQHATLEEHGAVSGETAIEMAYGARAALGADVGLSVTGIAGPGG
                     GTPEKPVGLVYVALSAGDWEQVERFVWSGSREENKAQSAEAALVMLRDYLSGRG"
     gene            complement(318..1649)
                     /locus_tag="AC162G_01885"
     CDS             complement(318..1649)
                     /locus_tag="AC162G_01885"
                     /inference="COORDINATES: protein motif:HMM:NF021436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HIR5074594.1"
                     /translation="MRYAIRDKRLAKGLLGIILLAYFAIAAQYAARTPDWQAPDEPAH
                     YNYIRYVVEHSALPVLRFGDYDQAYLERFTSPASTPDMSIDPLRYEFHQPPLYYLLAA
                     PVYALTGGDLLAMRMMSVALGGALIAVTYLVVCAIKPDRPDLALGTAAFVAFVPQHVA
                     MMSSVNNDSLAELLLALILLQTFRVVKAGKESELKELLVLGVLLGLGLVTKTTDYLAV
                     PIVLVALLLPALHRQPRNTQHATRNTYHMLLVFAPALLIGSLWWFRNINVYGDFDVLG
                     LQRHGAVVAGQPLTAGWIASMGFGPFLLGALTKTFHSFWGQFGWMGVPMPEWVYLGLG
                     VLSLIALAGWLLPVRPRRPVSHAAILLTLLVFFTFAAYIWYNLTFVQHQGRYLFPALI
                     PLGLGFSIGIACWTRVLPRPIGILVYVGVFAGLAALCVLALYRFVIPALSV"
     gene            complement(1679..3109)
                     /locus_tag="AC162G_01890"
     CDS             complement(1679..3109)
                     /locus_tag="AC162G_01890"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HIR5074595.1"
                     /translation="MNRLHRPVFATWIALSFVLLLGWTIARSDDDANPLHSRAFAPER
                     PLWGDDARANTDNSRMAQQEPHLAISRRNPDVMIVAAKDFRNTENITRAVWIYTSHDG
                     GRTWPWNQPFPNSSENIFRNSDPVAMARDDGRLYVLTLGTGRPVIENHGLFITWSDDD
                     GVTWREAVTVTAREDTGFDDKEWLAIDQSPRSPYYHRMYVAWRPAFYDGMWLASSADG
                     GLTWSDPITAAAGDIHAAYPIVDGNGRLLLFFVDPLKIDEPGAIRFVTSNDGGETFGL
                     PQHVADIRQPKSPLNEEDKFRVFSIISAAVDPSDPGRMYGAWTDARDAGSNGADAMLA
                     YSHDGGATWSEPMRLSGEPPGRVRDDFLPVIHAGARGRLHAFWMDRRGDPDNVLVNAV
                     YRASDDGGQTWGPTSRVSDLATDHNVAVPESSPSPGDYWGLDSMGNRVCASWTDTRHG
                     NEDVFVDCAWMADLEPPYYLPILLIE"
     gene            complement(3223..3816)
                     /locus_tag="AC162G_01895"
     CDS             complement(3223..3816)
                     /locus_tag="AC162G_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR5074596.1"
                     /translation="MESLQRFRVPIMIALTVIAGAVIYLVLRPAPAPPAITVTLRPSA
                     TVAPTPTARTTVRVYVSGAVLHPDVYSLPVDSIVRDAMIAAGGPASDADLDRINLAAP
                     LGDGMQVHFPRQGEPAAAQGGSVSGAAPAGATIDINTATLEQLDSLPGIGPVIAQRII
                     DYRTTNGPFASIEQIKEVKGIGDALFENIKDRITVGP"
     gene            complement(3912..>5548)
                     /gene="metG"
                     /locus_tag="AC162G_01900"
     CDS             complement(3912..>5548)
                     /gene="metG"
                     /locus_tag="AC162G_01900"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR5074597.1"
                     /translation="MVSGSDSHGTPITVQADKEGVTPREVFERYHLLFLETYQQLGIT
                     FDLFTHTDTENHHRVSQDMFLALHDHGYLFKQKQKQLYSEGAGRFLPDRYVEGKCPIC
                     GFDRARGDQCDNCGNLLDATQLIDPRSKIDDSTPVVRETEHYFFDLPAFTSRILEYLE
                     DKGYWRPNVMNFVLNYLREGLKPRPITRDLDWGIPVPLPEFQDKVLYVWFEAVIGYLS
                     ATIEWAKNNGTPDGWKEWWYDPSARTVYFVGKDNIPFHAIIWPAQLMGTQRLYEDDAS
                     KSLNLPADVPAAEFLNLEGQKFSTSRNWAVWTPDFLSRYDADALRYYLTANAPEQRDT
                     EFTWADFVRRNNDELVAAWGNLVNRVISLANKHFEGKIPGPGLMDHEDAQLLAQIDIA
                     FGPIGDLIGACKFKMAQGAVMAQAREVNKYLDTKAPWFQVKEDRVRAGTTLYIALRAI
                     DSLKTLFAPFLPFSSQRVHKMLGYGGDLLGRQVVRAYKESERSHQALTYDASSLTEKW
                     KPSQLPVGQAFGPIAPLFEKLDEKIIEEEKARLGVG"
BASE COUNT         1029 a         1712 c         1809 g          998 t
ORIGIN      
        1 gcgtgcagca cgcaacgctt gaagaacacg gcgcagtcag tggagagacg gcgatcgaga
       61 tggcgtacgg ggcgcgcgcg gccctcggcg cggacgtcgg cctgagcgtg acgggcatcg
      121 ccgggccggg cggcggcacg ccggaaaagc cggtcgggct ggtgtacgtc gcgctatcgg
      181 cgggcgactg ggagcaggtc gaacgattcg tatggagcgg aagtcgggaa gagaataagg
      241 cgcaatcggc tgaggcggcc ctcgtcatgc tgcgcgatta cctgtcagga cgcggataag
      301 cgcggattca cgcagattca caccgacagt gccgggatga cgaatcggta caaggcgagc
      361 acgcagagcg ccgccagccc ggcgaacacg cccacgtaaa cgaggatgcc gatcgggcgc
      421 ggcagcactc gcgtccagca cgcaataccg atcgagaacc ctaacccaag cgggatgagc
      481 gccggaaaca ggtagcgccc ctggtgctgg acgaaggtga ggttgtacca gatgtaggcg
      541 gcgaaggtga agaagacgag cagggttaac aggatcgctg cgtggctgac tggacgcctg
      601 ggccggacag gcaggagcca gccggcgagc gcgatcagag atagcacgcc aagtcccagg
      661 tacacccact ccggcatcgg cacgcccatc cacccgaact gtccccaaaa cgagtgaaag
      721 gtcttcgtca gcgcaccgag caggaacggg ccgaatccca tgctcgcgat ccagccggca
      781 gtgagcggct gcccggcgac gacagcgccg tgtcgctgca ggccgaggac gtcgaagtca
      841 ccatacacat tgatgttacg aaaccaccag agtgatccta tgagcagtgc aggcgcaaag
      901 acgaggagca tgtgatacgt gttgcgtgtt gcgtgttgcg tgttgcgtgg ctgtcgatgc
      961 agcgcgggca gtagtagagc cacgaggacg atgggcacag cgagataatc ggtcgtcttc
     1021 gtcacaagcc ccagtcctag caggactccc agcaccaaca gttccttgag ttcgctttct
     1081 ttccctgctt tcactaccct gaacgtctga agcaggatca aggcgagcag cagctccgcc
     1141 agcgagtcgt tgttgacgct agacatcatc gcgacgtgct gcggaacaaa cgcgacgaag
     1201 gccgccgtcc cgagcgcgag atcgggtcga tccggtttga ttgcgcacac gacgaggtac
     1261 gtgacggcga tcagcgcgcc gcccagcgca acggacatca ttcgcatggc gagcaggtca
     1321 ccgcctgtga gcgcgtacac cggcgcggcg agcagatagt acaagggcgg ctggtgaaac
     1381 tcgtaacgca aggggtcaat cgacatgtcc ggcgtgctgg ccgggctggt gaaccgttcg
     1441 agatacgcct ggtcgtagtc gccgaacctc agcaccggca gcgcactatg ctcgacgacg
     1501 tagcgaatgt agttgtagtg cgctggctcg tcgggcgcct gccagtccgg cgtgcgcgcg
     1561 gcgtactgag cggcgatggc aaagtacgcg agcaggatga tgccgagcag acctttggca
     1621 agtcgcttat cgcgtatcgc atatcgcatc ttcgctaaac cttcgcaagg gtgagcagtc
     1681 actcgatgag caggatgggc aggtaatacg gcggctccag gtcggccatc caggcgcagt
     1741 cgacgaagac gtcttcgttt ccatggcgcg tatccgtcca ggacgcgcat acgcgattgc
     1801 ccatgctgtc gaggccccag tagtctcccg gcgacggcga gctctccggc acggcgacgt
     1861 tgtgatccgt cgccaggtcg ctcacgcggc tcgtcggccc ccacgtctgg ccgccgtcgt
     1921 ccgacgcgcg atacaccgcg ttgacgagca cgttgtccgg atcgcctcgc cgatccatcc
     1981 aaaaggcgtg cagcctcccc cgagcgccgg cgtgaatcac cggcaggaag tcgtcgcgca
     2041 cgcgaccggg cggctcgccg ctaaggcgca tcggctcgct ccacgtcgca ccgccgtcgt
     2101 gcgaatacgc cagcatcgcg tctgcgccgt tggacccggc gtcccgcgca tccgtccagg
     2161 cgccgtacat gcggcccggg tcagagggat cgactgcggc agagataatg ctgaagacgc
     2221 ggaacttgtc ctcctcgttc aagggagact tcggctggcg aatgtcggcc acgtgctgcg
     2281 gaaggccgaa cgtctcgccg ccatcattcg acgtgacgaa gcggatcgcg cccggctcgt
     2341 cgattttcaa tggatcgacg aagaacagga gcaagcgacc attcccgtcg acgatcggat
     2401 aagcggcgtg gatgtctccg gctgcggccg tgatcggatc gctccacgtc agcccgccgt
     2461 cggcggagga tgccagccac atgccgtcgt agaaagccgg ccgccaggcg acgtacatgc
     2521 gatgatagta cgggctgcgc ggcgactggt cgatcgccag ccactctttg tcgtcgaaac
     2581 cggtatcttc ccgcgccgtg accgtcacgg cctcacgcca cgtcacgccg tcgtcgtccg
     2641 accaggtgat gaacaagccg tggttctcga tgaccggccg gcccgtgccg agcgtcagca
     2701 cgtacagccg cccgtcgtcg cgcgccatcg cgacggggtc gctgttgcgg aaaatatttt
     2761 cggacgagtt gggaaagggc tggttccacg gccaagtgcg gccgccgtcg tgcgaggtgt
     2821 agatccacac ggcgcgtgtg atgttctccg tgttgcggaa atccttggcg gcgacgatca
     2881 tcacgtccgg gttgcggcgg ctgatcgcca ggtgcggctc ttgctgcgcc atgcgggagt
     2941 tgtcggtgtt ggctcgcgca tcgtcgcccc acagcgggcg ttcaggagca aaggcgcggg
     3001 aatgcagggg gttggcgtcg tcatcgcttc gtgcgatcgt ccatccgagg agcaggacga
     3061 aactgagagc gatccatgtg gcgaagaccg ggcggtgtaa tcggttcaaa gcggaatcat
     3121 ctgaccggtg attagtaatc agttatcagt aatcagttat cagtaatcag ttatcggtgg
     3181 ccactggtta ctgatcactg atcactggtc actgataact gatcacggtc cgaccgtgat
     3241 gcggtctttg atgttctcga acagcgcgtc gccgatgccc ttcacctctt tgatctgctc
     3301 gatagaagcg aacggcccgt tggttgtccg atagtcgatg atgcgctgcg cgatcaccgg
     3361 cccgatgccg ggcagcgaat cgagctgctc gagcgtagcc gtattgatgt caatcgtcgc
     3421 gccggctggc gcggcgccgg aaacagaccc gccctgcgcg gcggccggct caccctggcg
     3481 cgggaagtgg acctgcatgc cgtcgccgag cggggccgcc agattaatcc gatcgagatc
     3541 agcgtcgctc gccggtccgc ccgcggcgat catcgcatcc ctgacgatgc tgtcgaccgg
     3601 cagcgagtat acgtcgggat gcagcaccgc gccgctgacg tacactcgca cggtcgtgcg
     3661 cgccgtcggc gtgggcgcaa cggtcgccga cgggcgaagc gtaacggtga tggccggagg
     3721 ggccggcgcg ggccgcagta ccaggtagat caccgcgccg gcgatgaccg tcagcgcgat
     3781 catgattggc acgcggaaac gctgcaggct ttccatagcg atatttctcg ctactcgtgg
     3841 acaatcaggt ctatgtcggc gtctgtcccc tccgctccgc gtcggggatc ttcgagagac
     3901 gcctcctaca actaacccac gcccagacgc gccttctctt cttcaatgat cttctcgtcc
     3961 agcttctcga acagcggcgc gatggggccg aacgcctggc cgaccggcaa ctggctcggc
     4021 ttccacttct ccgtcaacga cgacgcatcg tacgtcagcg cctggtgcga ccgttcggac
     4081 tccttgtacg cgcgcacgac ctgccggccc agcaggtcgc cgccgtagcc cagcatcttg
     4141 tgcacgcgct ggctggagaa gggtaggaac ggcgcgaaca gggtcttgag cgaatcgatc
     4201 gcgcgcagcg cgatgtacag tgtcgtgccg gcgcgcacac ggtcttcttt gacctggaac
     4261 cacggcgctt tggtgtcgag atacttgtta acctcgcgtg cctgcgccat cactgcgccc
     4321 tgcgccatct tgaacttgca ggcgcctatc aagtcgccga tcggcccgaa ggcgatgtcg
     4381 atctgcgcca ggagctgcgc gtcctcgtga tccatcagtc ccggcccggg aatctttcct
     4441 tcgaagtgct tgttggccag gctgatcacg cggttgacca ggttgcccca ggcggcgacc
     4501 agctcgtcgt tgttgcggcg cacgaagtcg gcccacgtga attcggtgtc gcgctgctcc
     4561 ggcgcgttgg ccgtgaggta gtagcgcagc gcgtcggcgt cgtaccgtga caggaaatcg
     4621 ggcgtccaca ccgcccagtt gcggctggtc gagaacttct gtccctccag gttgaggaac
     4681 tcggcggcgg gcacgtcggc cggcaggttc agcgatttgg atgcgtcgtc ttcgtaaaga
     4741 cgctgcgtgc ccatcaactg cgccggccag atgatcgcgt ggaatgggat gttgtccttg
     4801 ccgacgaagt agactgttct ggccgacggg tcataccacc actctttcca cccgtcgggc
     4861 gtgccattgt tcttggccca ttcgatcgtc gccgacaaat aaccaatgac cgcttcgaac
     4921 cacacataca agaccttatc ttgaaactcg ggcagcggca cggggatgcc ccagtcgagg
     4981 tcgcgcgtga tcgggcgcgg cttcaatccc tcgcggaggt agttgaggac gaagttcatc
     5041 acgttcggcc gccagtaccc cttgtcctcc aagtattcga ggatacggct ggtgaacgcg
     5101 ggcaaatcga agaaatagtg ctcggtctcg cgcacgaccg gtgtcgagtc gtcgatcttg
     5161 ctgcgcgggt cgatcaattg cgtcgcatca agcaggttgc cgcagttgtc acactggtcg
     5221 ccgcgcgcgc ggtcgaagcc gcagatcggg cacttgccct cgacgtagcg atcgggcagg
     5281 aagcggcccg cgccttcgga ataaagctgc ttctgcttct gcttgaacag gtagccgtgg
     5341 tcgtgcaacg ccaggaacat atcctgcgac acgcggtggt ggttctcggt atcggtatgc
     5401 gtgaacaggt cgaacgtgat gccgagctgc tgatacgttt cgagaaagag caggtggtag
     5461 cgctcgaaca cctcgcgcgg cgtcacgcct tccttgtcgg cctgcaccgt gatcggcgtg
     5521 ccgtggctgt cgctgccgga caccatca
//
LOCUS       DBNHXB010000051         3303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1016800, whole genome shotgun sequence.
ACCESSION   DBNHXB010000051 DBNHXB010000000
VERSION     DBNHXB010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1016800"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="AC162G_01905"
     CDS             <1..517
                     /locus_tag="AC162G_01905"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5074598.1"
                     /translation="GSTPVDETRLARLAAAAPAPLAEKMRAAHLEGERKQVTVLFADV
                     VGSTALAEQMDPEEWTAIMNRAFEQLSPAIYRYEGAIARLMGDALLAFFGAPLAHEDD
                     PMRALRAALDLLKAARAYGDELRRERGIEFAVRVGLNTGLVVVGAVGSDLKYEYTAMG
                     DAQIDLKRHSA"
     gene            514..1836
                     /locus_tag="AC162G_01910"
     CDS             514..1836
                     /locus_tag="AC162G_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5074599.1"
                     /translation="MIRLVPFRRLVDLAARIGADPADSDEIRLQKTLLVLGGSFMFIP
                     AGVIWGVIYFLFGEPLAGAIPLSYSIFSTISIVIFAFTRNYRFLRFSQLLLILLLPFL
                     LQVALGGFVNASAVIMWSLICPFGALLFGEPRDAPRWFVAYVGLVAASGLIQPYVRLV
                     NNLPPALVLAFFVANIAAVSAIAFTLLYHFVGQKDTFLRLLRVEQEKSENLLLNILPK
                     EIAAILKSENRTIADYFDGASILFADMVNFTPLTAAMAPRQMVDLLNEVFSYFDSLVE
                     KYDVEKIRTIGDSYMVAAGVPRPRPDHARVLARMALDIQAYVRSNPVCVDKHIDFRMG
                     LNSGPVVAGVIGRKKFIYDLWGDAVNTASRMESHGAPGQIQITRETYELIKDEFVCEP
                     RGKVMVKGKGEMETWYLVGMKDGLQSPAPAGSVTQRLTQDKTRVTDPL"
     gene            complement(1945..2937)
                     /locus_tag="AC162G_01915"
     CDS             complement(1945..2937)
                     /locus_tag="AC162G_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5074600.1"
                     /translation="MSSASTRSEQLPEFVIRSKDFEPAYMQLYRSGGLQRRACEAVAG
                     LAHCLACPRNCGVDRLADKTAACKTGRYARVSSYFPHVGEEDCLRGWNGSGTIFFSWC
                     NLRCVFCQNADISQAGEGRETRPERIAGMMLELQARGCHNINFVTPEHVVPQILEALA
                     LAVERGLRLPIVYNTSAYDSMESLHYMDGIVDIYMPDFKIWDSRTALRYLLAKDYPEA
                     ARQAIQEMHRQVGELKLDEQGLAKRGVLVRHLVMPGHIAGTREIMEFLAREVSPHTYV
                     NIMAQYYPAGRVTAEKFPEINRHITGKEYAQAVAVAREAGLYRFDERRMRVRWN"
     gene            complement(3008..>3303)
                     /locus_tag="AC162G_01920"
     CDS             complement(3008..>3303)
                     /locus_tag="AC162G_01920"
                     /inference="COORDINATES: protein motif:HMM:NF028226.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HIR5074601.1"
                     /translation="EGLPDKIKTAVEAIPGVVDCHRVRVRLSGPHLFVDAHVALAGNP
                     PLSVAHDMVLKIEEAIHHVAPRADIVVHADPEATVTARLMRKDAAEELRYTPD"
BASE COUNT          581 a         1074 c         1005 g          643 t
ORIGIN      
        1 cggcagcacg ccggtcgacg aaacgcgcct ggcccgcctc gccgccgctg cgccggcgcc
       61 gctggctgag aagatgcgcg ctgcccacct ggaaggtgag cgaaaacaag tgaccgtctt
      121 gttcgccgac gtagtcggct cgaccgcctt ggcagagcaa atggaccctg aagaatggac
      181 ggcgatcatg aatcgcgcct tcgaacagtt gtcgcccgcg atttaccgct acgagggcgc
      241 gatcgcgcgg ttgatgggag atgctctgct ggccttcttc ggcgcgccgc tcgcccacga
      301 ggacgatccc atgcgcgcgc tccgcgccgc gctcgatctg ttgaaagcgg cgcgggcata
      361 cggcgacgag ctgcgccgcg agcgcggcat tgagttcgcg gtgcgcgtcg gcttgaacac
      421 cgggctggtc gtcgtcggcg cggtgggcag cgacctgaag tacgagtata ccgcgatggg
      481 cgacgcgcaa attgatctga aacgccattc ggcatgattc gcctcgtccc gttccgcagg
      541 ctcgtcgatc tggctgcccg catcggcgcg gaccccgccg acagcgacga gattcgcctt
      601 cagaagacgc tcctggtgct cggcggcagt ttcatgttca ttcccgccgg cgtgatctgg
      661 ggtgtgattt acttcctctt cggagagccg ctcgccggcg cgattccgct atcctactcc
      721 atattctcga cgatcagcat cgtcatcttt gcgtttacgc gcaactatcg attcctgcgt
      781 ttcagccagc tcctcctgat attgctgctg cccttcttgc tccaggtggc gctgggcggc
      841 tttgtcaacg ccagcgccgt catcatgtgg tcgctgattt gtccttttgg tgcgctgctc
      901 ttcggcgagc cgcgcgacgc tccgcgctgg ttcgtcgcct atgtcggcct ggtcgcagcc
      961 agcggcctga tccagccgta cgtgcgactc gtgaacaatc tgccgcctgc gctggtgctg
     1021 gccttcttcg tcgccaacat cgccgccgtc tcggccatcg ccttcacgct gctctatcac
     1081 ttcgtcgggc agaaagacac gttcctcagg ctgctgcgcg tcgaacagga gaaatccgag
     1141 aatctgctgc tgaatatcct gccgaaggag atcgcggcga tcctgaagag tgagaatcgt
     1201 accatcgccg attacttcga cggcgccagc attttgttcg ccgacatggt gaacttcacg
     1261 ccgctgacgg cggcgatggc gccacggcag atggttgatc tgctcaacga agtcttctcc
     1321 tacttcgatt cgctggtcga gaagtacgac gtggagaaaa tcaggacgat tggggacagc
     1381 tacatggtcg ccgccggcgt gccgcgcccg cgcccggatc acgcccgcgt gctcgcgcgc
     1441 atggcgctcg acatccaggc gtacgtgcgc agcaacccgg tctgcgtcga caagcacatc
     1501 gacttccgca tgggcctgaa ctccggcccg gtcgtggcgg gcgtgatcgg ccgcaaaaag
     1561 ttcatttacg acctgtgggg cgacgcggtg aacaccgcca gccgcatgga gtcgcacggc
     1621 gcgcccggcc agattcagat cacgcgcgag acctacgagt tgatcaagga cgagtttgtc
     1681 tgcgagccac gtgggaaggt gatggtaaaa ggcaagggcg agatggagac gtggtatctc
     1741 gtgggaatga aagacgggct acagtctccc gctcccgctg ggtctgtgac ccagcggctc
     1801 acacaggata agacgcgggt cacagacccg ctttgagcat gcaaacctac caccaagaca
     1861 cgaagacacc aagaaaaact ctgaaatcct tcgtgtcttg gtgtcttcgt ggttaagaag
     1921 agccaattta agacgcgctc tgagtcaatt ccaccgtacc cgcatgcgcc gctcgtcgaa
     1981 gcgatacagc ccggcttcgc gcgctacagc cacagcctgc gcatattcct tgccggtgat
     2041 gtggcgattg atctccggga atttctctgc ggtcacccgt cctgccggat aatattgtgc
     2101 catgatgttc acgtaagtgt gcggcgagac ctcgcgggcc aggaactcca taatttcgcg
     2161 cgtgccggcg atgtgccccg gcataaccag atgccgcacc agcacgccgc gcttcgccag
     2221 cccctgctcg tccagtttca gctcgccgac ctgccggtgc atctcctgga tagcctgtcg
     2281 cgccgcttcc gggtaatctt tggccagcaa gtacctcagt gccgtccgcg aatcccagat
     2341 cttgaaatcc ggcatgtaga tgtcgacgat gccgtccatg taatgcaggc tctccatcga
     2401 gtcgtaggcc gaggtgttgt aaacgatcgg caggcgcagc ccgcgctcga cggcgagcgc
     2461 cagcgcttcc aggatctggg gcacgacgtg ctcgggcgtg acgaagttga tgttgtggca
     2521 gccgcgcgcc tgcagctcca gcatcatccc cgcgatgcgc tccggccggg tctcgcgccc
     2581 ctcgccggcc tggctgatat ccgcgttctg gcagaagacg cagcgcaaat tgcaccacga
     2641 gaagaagatc gtcccgctcc cgttccagcc gcgcaggcag tcttcctcgc cgacgtgcgg
     2701 aaaataactg ctcacccgcg cgtatcgccc ggtcttgcag gcggccgtct tgtcggccag
     2761 ccgatccacg ccgcagttgc gcgggcaggc caggcaatgc gccaggccgg cgactgcttc
     2821 gcaggcgcgg cgctgcaatc ccccgctccg gtagagctgc atgtaagctg gctcgaagtc
     2881 cttcgaccgt atgacgaact caggcaattg ctctgaccgg gtcgatgccg acgacatctc
     2941 accctccgtg cctgctcacg cgccgggcac gcccttgcga tacgggcatt ataccccacc
     3001 cccgagatca atcgggtgtg taacgcagtt cttccgccgc gtccttgcgc atcaagcgcg
     3061 cggtcacagt cgcctctgga tcagcatgca ccacgatgtc ggcgcgcggc gcgacgtgat
     3121 gaatagcctc ttcgattttg aggaccatgt cgtgcgcaac gctcagtggg ggattgccgg
     3181 ccagggccac gtgcgcatcg acgaacagat gcgggccgga caggcgcaca cgcacgcgat
     3241 ggcagtcgac cacgccgggg atggcctcga cggcagtttt gattttgtcc ggcagccctt
     3301 ccg
//
LOCUS       DBNHXB010000052         4484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_10791, whole genome shotgun sequence.
ACCESSION   DBNHXB010000052 DBNHXB010000000
VERSION     DBNHXB010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_10791"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..158
                     /locus_tag="AC162G_01925"
     CDS             9..158
                     /locus_tag="AC162G_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074602.1"
                     /translation="MLTAIGADVISGDVSFINALIRHRIDEQTTLTVWREGETLTLNV
                     VLKAR"
     gene            complement(181..1797)
                     /locus_tag="AC162G_01930"
     CDS             complement(181..1797)
                     /locus_tag="AC162G_01930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR5074603.1"
                     /translation="MNDRPRSHSTTGNLAGPPRPVSWLLLVILFGFAIRFAAIDFQSL
                     WRDEIDAGGFGAPEMRMGEVLAAGDFQTLAQSLTQGGFNGPLYFIALQQWTRVAGDTA
                     FALRFPSALFGALAIPLIYVLTRRLLRGTPIDGFSRHVALIAAWLVATSPYFVWYSQE
                     AKMYTEVTALAIAAIYALCRAVDAAESRRAWPWWALAVAATTLAMYSHILAALLIGVE
                     IALFLLWWPQSKRHWLGGLIALAALTLPYLPLIGWQLTYALTPGSQGFAFYRFDQILQ
                     VMLAAFANGVQPFDYSLSTIGVEWDAGPMTPALRPTMWGLWLLSLLAIAGAVTWRDKR
                     DRSQRIGLAAWALIPMAIIALVSLNRPIFTDRYLIWIGPAIYLLAALGIVEAGHWNRA
                     LGRAALVAITGVALVSTLAQSTAPYKADFRSAASYVEKHYRGEVIVFQIPYGRYNFDY
                     YFEPPFPFLEGPYTNYRNPDGSYQIDPAAADAHIAGQLGGIESVWLVATEVEMWDERH
                     LLQEWLGRNGRVTDQADFMRVTVARYELGP"
     gene            complement(1787..2047)
                     /locus_tag="AC162G_01935"
     CDS             complement(1787..2047)
                     /locus_tag="AC162G_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074604.1"
                     /translation="MSRFVLFALFVSCATAILGLPLASIPQWQSPVATPAPPASPLPT
                     STSTATLEASGAVTFLLVGIAVGLILGAAVVLIVQRAAPNER"
     gene            2144..>4484
                     /locus_tag="AC162G_01940"
     CDS             2144..>4484
                     /locus_tag="AC162G_01940"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR5074605.1"
                     /translation="MRILRTVLVLVLIVVIVLGMVGVGGFFYLARRPLPQTDGTTQLA
                     ALNAPVTVIRDKFGIPHIYASSVHDLFFAQGYVHAQDRLWEIEAGRMGISGRTSELRP
                     SVSALEQDKFVRTLGWRRAAEADFQAASEQSQAILQAYADGFNAFISTHQDSLPIEFF
                     IVGLFGSKGPGYKPDPWTPVDSLQWAKVMAWRLSGNWENEWLRSQILAKFGEEKGKTM
                     IADLEPPYDYEKRPIIVPEDVAWERVPAEVRELRTLDEISGPRGNDIGSNSWAVAGSR
                     STTGMPLLANDPHLGYQMPALWYFNSLHCEPVGPDCPFDVIGASLPGAPGIIIGHNAR
                     IAWSMTNVGPDVQDLFLEKVDGNQYEFQGTMLDLTIVPETWTIKGKLPEGYEPSPNET
                     AEYDETSNTTTITLNVRYTRHGPLISDVDATAAEIDPQYAVAFAWTAINAPEHTLESV
                     LRFNAAQNWEEFREALRVFGTPSQNMVYADVDGNIGYQTPGRIPIRARGDGLLPVPGW
                     TGEYEWTGYIPFDGLPSAFNPAQGYIATANNAVVEPDYPYFLTTEWNRGYRARRIVDL
                     IEAKDKLSPDDMAAIQGDNFSLSAPDIVAYLDGLAVDGGGQKALDAIRSWDFNDVIDS
                     TGAAAYEVFWLYLLRNTFDDDLGDLAADYVDGGDVNSQAVTQLLAQPDAGWWDDKTTP
                     DVVETRDDILEKSLADAAAVLTAEFGADPAGWKWGLLHRVTFASQALGDSPVAFIFNR
                     GPFDVNGGAELVNNNGTSFGQAYPDDPDDPAPEFTSIFQS"
BASE COUNT          884 a         1421 c         1443 g          736 t
ORIGIN      
        1 cgggcgacat tctcacggcg atcggcgccg acgtcatcag cggcgacgtg tctttcatca
       61 acgcgttgat ccgccacagg atcgacgagc agacgacgct cacggtgtgg cgggagggtg
      121 aaacgcttac gttgaacgtg gttctgaaag cacgatagtg aacaggaatg ccgcctcgtc
      181 tcacggaccc agctcgtacc gcgcgacggt tacgcgcatg aagtccgctt ggtcggtaac
      241 gcgcccgttg cgtcccagcc actcctggag caggtggcgc tcgtcccaca tctccacctc
      301 ggttgccacc agccagaccg actcgatccc cccgagctgg ccggcgatgt gcgcgtccgc
      361 cgccgcaggg tcgatctggt agctgccgtc gggattgcga taattcgtgt acgggccttc
      421 cagaaatgga aatggcggct cgaagtagta atcgaagttg taccggccgt acggaatctg
      481 gaagacgatc acttcacccc gatagtgttt ctccacgtag ctcgccgccg atcggaagtc
      541 ggccttataa ggtgcggtcg actgcgcgag cgtgctgacg agcgcgacgc ccgtgatggc
      601 gacgagcgct gcgcgcccca gcgcccggtt ccagtgtcct gcttcgacga tccccagcgc
      661 cgccagcaag tagatcgccg gtccgatcca gatgaggtat cgatccgtaa aaatgggccg
      721 gttgagcgac accagcgcga tgatcgccat cgggatgagc gcccacgctg ccaacccgat
      781 gcgctggctg cgatccctct tgtccctcca ggtgactgcg ccggcgatcg ccagcaagct
      841 cagcagccac agcccccaca tcgtcgggcg cagcgccggc gtcatcgggc cggcatccca
      901 ctcgaccccg atagtgctga gcgaataatc gaagggctgc acgccgttgg caaacgcggc
      961 gagcatcacc tggaggatct ggtcgaagcg atagaatgcg aagccttgac tgcctggagt
     1021 caaagcatac gtcagttgcc acccgatgag tggcaagtag ggtagcgtga gcgcggcgag
     1081 cgcgatcaag ccacccagcc aatgccgctt cgattgcggc caccacagga gaaatagtgc
     1141 gatttccacc ccgatcagca gtgcggccag gatgtggcta tacatcgcca gcgtggtcgc
     1201 cgcgacggcg agtgcccacc acggccacgc gcgccggctc tccgcggcgt cgacggcccg
     1261 gcacagcgcg tagatcgcgg cgatcgccaa ggccgtgacc tcggtgtaca tcttggcctc
     1321 ttgcgaatac cacacgaaat acggcgaggt ggccaccagc caggcggcga tcaaggcgac
     1381 gtggcgagaa aatccgtcga tgggcgtccc acgcagcagg cgacgggtca ggacgtagat
     1441 caggggaatg gcgagtgcgc cgaaaagggc ggacgggaag cgcagcgcga aagccgtgtc
     1501 gcctgccacg cgcgtccact gctgcagcgc gatgaaatac agcggcccgt tgaacccgcc
     1561 ctgggtgagc gattgcgcga gcgtttgaaa gtcgccggca gccaacacct cgcccatacg
     1621 catctcaggc gcgccgaagc cgcccgcgtc gatctcgtcg cgccacaagc tctggaagtc
     1681 gatcgcggcg aagcgaatgg cgaaaccgaa caggatcacg agcagcagcc acgacacggg
     1741 gcgcggcggc ccggccagat tccccgttgt ggaatggctg cgcggcctat cgttcattcg
     1801 gagctgcgcg ctggacgatc aagacgacgg cggcgccgag gatcagcccg acggcgatcc
     1861 cgacgaggag gaacgtgacc gccccgcttg cctcgagcgt ggccgtcgaa gttgacgtcg
     1921 gcagcggact ggctgggggc gcaggcgtgg cgacgggcga ctgccattgc ggaatagacg
     1981 ctaaaggcaa gcccaggatg gcggtggcac aagacacgaa cagggcgaaa aggacgaatc
     2041 gactcacggc atggattata gtgaaccttg tcggctttga caatcgtcgt gaagtatgtt
     2101 acaaagacat tatccccgcc ctgcatcacg gtatggaggc aaaatgcgca tacttcgcac
     2161 agtccttgtg cttgtcttga tcgtcgtcat cgtcctcggc atggtcggcg tcggggggtt
     2221 cttttacctg gcgcgccgcc cgttgcccca gaccgatggc accacgcaac tggccgcgtt
     2281 gaatgccccg gtcaccgtca ttcgcgacaa gttcggcatt ccgcacattt atgcgagcag
     2341 cgtgcacgat ctcttctttg cgcaaggcta cgttcacgcg caggatcgcc tgtgggaaat
     2401 agaggccggg cgcatgggca tctccggccg cacctccgag ctgcgcccgt cggtgtcagc
     2461 gctcgagcaa gacaagttcg tccgcacact cggttggcgc cgagcggccg aggccgattt
     2521 tcaggccgcg agtgaacagt cccaggcaat ccttcaggcg tacgccgacg gcttcaacgc
     2581 cttcatctcg acgcaccagg acagcctgcc gatcgaattc ttcatcgtcg gcttgttcgg
     2641 cagcaagggg ccgggataca agccagaccc gtggacgccg gtcgactcgc tgcaatgggc
     2701 caaggtgatg gcctggcggt tgagcggcaa ctgggagaac gagtggctgc gctcgcagat
     2761 tctcgcgaag ttcggcgagg aaaagggcaa gaccatgatc gccgatctag agccgcctta
     2821 cgattacgag aagcggccga tcatcgtgcc cgaagatgtc gcctgggagc gcgtgccggc
     2881 cgaggtacgt gaactgcgca cactggatga aattagcggc ccacgcggca acgacatagg
     2941 ctcgaacagt tgggccgtcg ccggcagccg ctcgacgacc ggcatgcccc tgctcgccaa
     3001 cgatccgcac ctcggctacc agatgccggc gctatggtat ttcaacagtc tccactgtga
     3061 gccggttggg ccggactgtc ccttcgacgt gatcggcgcg tcgctgccgg gcgcgcccgg
     3121 catcatcatc ggccacaacg cgcgcatcgc ctggagcatg acgaacgtcg gcccggacgt
     3181 gcaagacctg tttctggaga aggtcgacgg caaccagtac gagttccagg gtacgatgct
     3241 cgacctgacg atcgttcccg agacgtggac gatcaaaggc aaactgcccg aagggtacga
     3301 gccgtcgccg aacgagaccg ccgaatacga cgaaacgtcg aacaccacga cgatcacgct
     3361 caatgtgcgt tacacgcggc acggcccgct catcagcgac gtcgacgcga ccgcggcgga
     3421 gatcgatcca cagtacgcgg tcgctttcgc ctggacggcg atcaatgcac cggagcacac
     3481 gctcgaatcc gtgctgaggt ttaacgccgc tcagaactgg gaagagttcc gcgaggcgct
     3541 gcgtgtcttc ggcacgccca gccagaatat ggtctatgcg gacgtggacg gcaatatcgg
     3601 ctaccagacg ccgggccgca tcccgatccg cgcccgcggc gatggcctgc tgccggtacc
     3661 gggctggacc ggcgagtacg agtggaccgg ctacatacca ttcgatggac tgccgtctgc
     3721 cttcaacccg gcgcagggat acatcgccac ggcgaacaac gcggtcgtcg agccggacta
     3781 cccgtacttc ctgacgacgg aatggaaccg tggctaccgc gcccggcgca tcgtcgatct
     3841 catcgaggcg aaggacaagc tcagcccgga cgacatggcg gccattcagg gcgacaattt
     3901 cagcttgtcc gcgccggaca tcgtggccta tctcgacggt ctcgccgtcg acggcggcgg
     3961 gcagaaggcg ctcgacgcca tccgaagctg ggatttcaat gacgttatcg acagcacagg
     4021 cgcggccgcc tacgaggtgt tctggctgta cttgctgcgc aacaccttcg acgacgatct
     4081 cggtgacctt gcggctgact acgtcgacgg cggcgacgtg aatagccagg cggtgacgca
     4141 gttgctggcg cagcccgatg cggggtggtg ggatgacaag acgacgcccg acgtggtcga
     4201 gacgcgagat gacattctgg agaagtcgtt agcggacgcc gcagccgtgc tcacggccga
     4261 gtttggcgcc gacccggcgg gctggaagtg gggcctgctg caccgggtga cgttcgccag
     4321 ccaggcgctg ggcgacagcc cagtcgcgtt cattttcaac cgtgggccgt tcgacgtgaa
     4381 cggcggagcc gagctggtta acaacaatgg caccagtttc ggccaggcgt atccggacga
     4441 tcccgacgac cctgcgcccg agttcacgtc tatcttccag tcga
//
LOCUS       DBNHXB010000053         3576 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_682018, whole genome shotgun sequence.
ACCESSION   DBNHXB010000053 DBNHXB010000000
VERSION     DBNHXB010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_682018"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..318)
                     /locus_tag="AC162G_01945"
     CDS             complement(124..318)
                     /locus_tag="AC162G_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074606.1"
                     /translation="MKITVDFKAEGGQSIGAVTVSPKAFKTGSKGFYGQGKIEFEGKR
                     YQVQVQLVEIGSKERAKAGA"
     gene            complement(448..1635)
                     /locus_tag="AC162G_01950"
     CDS             complement(448..1635)
                     /locus_tag="AC162G_01950"
                     /inference="COORDINATES: protein motif:HMM:NF020371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIR5074607.1"
                     /translation="MSAEPETQPAQSATQKYTCPKCGGDMNFDAAKGLLSCPFCGHTM
                     PTPETSEVVQEHDLVQALRDTSGKARGFGTAVRSYKCQACGATNNVDPHVTSTACPFC
                     GSNQVLEQEPDPDLIQPESLVPFGVDEARAHQMFRDWLGRGLFTPNDLKKTGGGQRLA
                     GVYLPFWTFDAHAESDWRAESGEYYTVHETVWVTRDGKQVQEVRPVQKIRWYPSSGRH
                     AARYDDVLVYATNSVDVTILEKVYPFDTKKLEPYNPSYLSGWGAESYRIALSQAWELG
                     QSIIQREEYNACGREVPGDTHRNLRVSTHLSHLKYKHVLLPVWLASYRYRNKVYRFMI
                     NGQTGEVQGQKPISWIKVAIAVVIALIVIAIVLYLVSQSQSSSGELLSYYPYAMEHML
                     PVG"
     gene            complement(1704..>3576)
                     /locus_tag="AC162G_01955"
     CDS             complement(1704..>3576)
                     /locus_tag="AC162G_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074608.1"
                     /translation="RRAAYFFVLTSTPIMLLPTALAVNEITPSNLRAMGMMPMVFVVP
                     ALGTWFIFKWLIALRRKTMDEGRNIVVRRAKRSFVAVLLLVVLLGAADTASTYAGYAR
                     LPELYYASDGDLVDIAHLLNRADLQQTTAYVHALHYRHPTLAALAQSFHEIRSITGPD
                     VLVFPQGRSTHIFANLAQPADRAWRERFLPPPARLDAAIGPDGRTAYEIVTVDAQPSI
                     RPQNMVDANFGDTIRLLGYDIESRATSGGEIDVTLIWEVLNRPDRGDYATFAELRDAW
                     GFQWGQAGSFDYPSEQWTPGEIIVQRLRVPIADGAPRGDYRLDAGWHAGDRRLPVVKA
                     GGSFGGTTASLGPIPIARARREPSKGSLGMGTRLGVHPIDGLFLLGSSLETPVAPQGG
                     PLIFTLFWRVESHVPDQPVGIRMSAPGDVIYALSTASPVHDTYPFDEWRPGEVVADRY
                     GLRVPVDALPGEYALEARVRPGKWIRFGTVRVEETQRRFDVPPIAHPAGATFGDQIEL
                     LGYDLNETREAIELTLYWRALKTPDDDYTVFTHLLDAAGVQRGGKDNPPVNGTYNTSL
                     WIEGEVIEDTYTIPIPAGAPPGEYTVEVGLYRFDTGERLPLSSGGDALRLATIEIAP"
BASE COUNT          610 a         1200 c         1111 g          655 t
ORIGIN      
        1 gccacttctc caggtgggcg ttgtcaagcg agacgcgccg agtgttcctg ccggcggtct
       61 tgaagaactg atggacgggc tgattaacca aaaggtgcag tatgccgtgg gatggctgga
      121 ttctcacgcc cccgcttttg cccgctcttt gctcccaatc tccaccaact gcacctgcac
      181 ttgataccgc ttcccctcga actcaatctt gccctggccg taaaacccct tcgagccggt
      241 cttgaacgcc ttgggcgaga ccgtcaccgc cccgatcgat tgcccaccct ccgccttgaa
      301 gtcgacggtg atcttcatgc acataccccc gatctgagct ttgtctgccg ctggtggcct
      361 atcgtcaacc gagccgggtg agccagggca tccttgcgtc ccaacgtatc caccgctatc
      421 cgactacgcg actacgcgac catcagacta cccgactggc aacatatgct ccatcgcata
      481 cgggtaatat gacagcaact caccgctgct gctctggctc tggctcacca gatacaagac
      541 gatggcgatg acgatcagcg cgatcacgac ggcgatcgcc accttgatcc acgagatcgg
      601 tttttgcccc tgcacctcgc cggtctggcc attgatcatg aagcgataga ctttgttccg
      661 gtagcggtac gaggccagcc ataccggcag cagcacgtgc ttgtatttca gatgcgacag
      721 gtgcgtgctg acgcgcaggt tgcggtgagt gtcgccgggc acttcgcggc cgcaagcatt
      781 gtattcttcg cgctggatga tgctctgtcc caactcccaa gcctggctta gggcgatgcg
      841 ataggattcc gcaccccagc cggacagata actcgggttg tacggctcga gttttttcgt
      901 gtcgaaggga tagacctttt cgaggatcgt gacgtcgacc gagttggtcg cgtacaccag
      961 cacgtcgtcg tagcgggcgg cgtggcgccc gctcgacggg taccagcgga tcttctgcac
     1021 ggggcgcact tcttgcacct gcttgccgtc gcgcgtcacc cacaccgtct cgtggacggt
     1081 gtagtattcg cccgactccg cacgccagtc gctctccgcg tgcgcgtcga acgtccagaa
     1141 cggcaggtac acgccggcga gtcgctgccc gccgccggtc ttcttcaggt cgttgggcgt
     1201 gaacagcccg cgccccagcc agtcgcggaa catctggtgt gcgcgcgctt cgtccacccc
     1261 gaaggggacg agggattcgg gctggatcag gtcgggatca ggctcctgct cgagcacctg
     1321 gtttgatccg cagaaggggc acgccgtcga cgtgacgtgc ggatcgacgt tgttcgtcgc
     1381 gccgcaggcc tggcacttgt acgaccgcac cgccgtaccg aagccgcgcg ctttgccgga
     1441 cgtgtctctc agcgcctgaa cgagatcgtg ctcctgcacg acttccgacg tctcgggcgt
     1501 gggcatcgtg tggccgcaga acgggcaact gagcaggcct ttggcggcgt cgaaattcat
     1561 gtcgccgccg cacttggggc aggtgtattt ttgagtagcg gattgggcgg gctgtgtttc
     1621 aggctctgcg ctcacgggaa acctcctcaa gacttccgaa gtccggcgta agtatactca
     1681 catggcgaca gacgacaaaa ctatcacggc gcgatctcga tcgtcgccag ccgcagcgca
     1741 tcgccgccgc tcgacagcgg cagtcgctcg ccggtgtcga accgatacag cccgacctcg
     1801 accgtgtact cgcccggcgg cgcgccggcg gggatcggga tcgtgtaggt gtcctctatc
     1861 acttctcctt cgatccacag cgaggtgttg tacgtgccgt tcacgggcgg gttatccttt
     1921 ccgccgcgct gcacgcccgc cgcgtcgagc aaatgggtga agacggtgta atcgtcgtcg
     1981 ggcgtcttca gcgcgcgcca gtacagcgtc agctcgatcg cctcgcgggt ctcattcagg
     2041 tcatatccaa gcagctcgat ctgatcgccg aacgtcgcgc cggcgggatg cgcgatcggc
     2101 ggcacgtcga accggcgctg cgtctcttcg acgcgcaccg tgccaaaccg gatccacttg
     2161 cccggccgga cgcgcgcctc cagcgcatat tcgcccggca gcgcatccac cggcacgcgc
     2221 agcccgtagc ggtcggcaac cacctcgcct ggccgccatt cgtcgaatgg gtaggtatcg
     2281 tgcacgggcg aggccgtcga caacgcgtag atcacatcgc ccggcgccga catgcgaatt
     2341 ccgaccggtt gatcgggcac gtggctctcc acccgccaaa acagcgtgaa gatcagcggc
     2401 ccaccctgcg gcgcgaccgg cgtttcgagc gacgaaccca ataggaacaa cccatcgatc
     2461 ggatggacgc cgaggcgcgt tcccatgccc aacgatccct tcgacggctc acgtctcgcg
     2521 cgcgcgatcg ggatcgggcc gaggctggcc gtcgtcccgc cgaagctgcc gccggccttc
     2581 acgacgggca ggcgtcgatc gcctgcgtgc cagccggcat ccagtcgata atcgccgcgc
     2641 ggcgcgccgt cggcgatcgg cacgcgcagg cgctggacga tgatttcgcc cggcgtccac
     2701 tgctcggatg ggtagtcgaa cgaaccggcc tgcccccact gaaaacccca cgcgtcgcgc
     2761 agctcggcga acgtcgcgta atcgcctcgg tcgggccggt tgagcacctc ccagatcagc
     2821 gtcacgtcga tctcgccgcc ggacgtcgcc cgcgattcta tgtcgtagcc gagcagtcgg
     2881 atcgtgtccc cgaagttggc gtcgaccatg ttctgcggcc ggatggaggg ctgcgcatca
     2941 accgtcacga tctcgtaggc cgtccggcca tccgggccga tggccgcgtc gaggcgcgcc
     3001 ggcggcggca ggaatcgctc gcgccaggct cgatccgccg gctgcgcgag gtttgcgaag
     3061 atgtgcgtag accggccctg cgggaatacc agcacgtccg gcccggtgat cgaccggatc
     3121 tcgtggaaac tctgcgccag cgccgcaagc gtcgggtggc gatagtggag cgcgtggacg
     3181 taggcggtcg tctgctgcag atcggctcgg ttcagcaggt gggcgatgtc gacgagatcg
     3241 ccgtcgctcg cgtaatacag ctccggcagg cgcgcgtagc cggcgtacgt cgaagccgtg
     3301 tcggccgcgc ccagcaggac gacgagcagc agcacggcga cgaacgaccg cttcgcgcga
     3361 cgaacgacga tattccgtcc ttcgtccatc gtctttcgtc gcagcgcgat cagccatttg
     3421 aagatgaacc acgtccccag cgccggtacc acaaacacca tcggcatcat gcccatcgcg
     3481 cgcaggttgc tcggcgtgat ctcgttgacg gctaatgccg tcggcagcag catgatcggc
     3541 gtcgaggtca gaacgaagaa atacgccgcg cgccgc
//
LOCUS       DBNHXB010000054         5200 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3701797, whole genome shotgun sequence.
ACCESSION   DBNHXB010000054 DBNHXB010000000
VERSION     DBNHXB010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3701797"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="AC162G_01960"
     CDS             complement(<1..333)
                     /locus_tag="AC162G_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074609.1"
                     /translation="MSKPWIILSLVALGFILAACSGSTSSAPGPDRLTPKPADTAGSA
                     SESAAQPAANAMQFNDEAAVRVEVTPLNLDDPSAATIDFRIAMNTHSVDLNYDLTKIA
                     TLSSDAGEE"
     gene            complement(330..860)
                     /locus_tag="AC162G_01965"
     CDS             complement(330..860)
                     /locus_tag="AC162G_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074610.1"
                     /translation="MNEAFDTVAVRSRLLTWATREARWHKYRLPILMGVGGALALFAL
                     YMTIVTLAQGWEHAVELFGQDAWLVIPIMAGFGTQVGLYTYLRGVLRHGSRASKAMMG
                     AGGGTSTAAMVACCAHHVADLLPLLGLSAAASFLAAYKIPFMLVGLAMNVIGIIIIVR
                     TIRKEQRHTQMMESHS"
     gene            complement(1061..1279)
                     /locus_tag="AC162G_01970"
     CDS             complement(1061..1279)
                     /locus_tag="AC162G_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016881559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR5074611.1"
                     /translation="MFGMGLGGGLIMILFWVGLIVGAVWLAQALFNSGRTSSSTGRDL
                     TAKEILDQRFARGEITREEYDLMKQDLQ"
     gene            complement(1298..1552)
                     /locus_tag="AC162G_01975"
     CDS             complement(1298..1552)
                     /locus_tag="AC162G_01975"
                     /inference="COORDINATES: protein motif:HMM:NF021378.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR5074612.1"
                     /translation="MMTGFGDFGTFGWIGMILNWVITIGLILGVVLLIAWLVRRIDAN
                     GRASQAAPSSVSPRDILQVRYARGEITREQYQQMLADLNT"
     gene            complement(1653..2786)
                     /locus_tag="AC162G_01980"
     CDS             complement(1653..2786)
                     /locus_tag="AC162G_01980"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5074613.1"
                     /translation="MIASHIRRHLGWKIFLSYLIVIVVGVAVLATAANFAAPGAFQRH
                     IAGMDSMMMGGQMMDMGDLLADFRGAVSEALTLAALAALIGAVAVSLFVSRQVVAPVR
                     AMTAATQRIAGGHYGERVRVSGDPAKNELDELAQLALHFNQMADKLERTESMRRQLIG
                     DVAHELRTPLTAIKGSMEGLIDGVLPAEVETFQQVYREADRLQHLVADLQELSRVEAG
                     AYSLNRRPVQVHQLVDTLAARLGRQFTEKQVALETDLPAGLPAVMVDEDRIGQVLLNL
                     AGNALQYTPAEGKVRVAARRHGGEIYIFVSDTGIGIPPEHLPHVFTRFYRVDKSRSRA
                     GGGSGIGLTIAKHLVEAHGGRMWAASPGPGEGSTFTFTLPIAN"
     gene            complement(2783..3469)
                     /locus_tag="AC162G_01985"
     CDS             complement(2783..3469)
                     /locus_tag="AC162G_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5074614.1"
                     /translation="MSSTKILLVDDEQSILNLATAYLQKEGYETYTAMDGPSALKAAR
                     AFRPDLIVLDIMLPGMDGLEVLSHLRRESDVYVILLTAKSEEADKVIGLSVGADDYLT
                     KPFSPRELVARVKAALRRLRTGASLLSGEVLAFRHVRIDTGSRQVWLDDEPVELTTIE
                     FDLLKTLAEHHGKVLSREQLLERVWGYDYYGEIRVVDVHVGHIRQKLGEPFIATVRGV
                     GYRFEDEGVG"
     gene            complement(3595..4461)
                     /locus_tag="AC162G_01990"
     CDS             complement(3595..4461)
                     /locus_tag="AC162G_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M4"
                     /protein_id="HIR5074615.1"
                     /translation="MNRTLRWTLIIVGIVVVAAALVWSGVLIARGASGWAGYGPTMMG
                     RSYGYDPDQTGSTQVPYGYGMGPGMMGYAQTYSGTLPHGPGMMGGGMMGGSGMMGRGM
                     MGGYSGSLYGVEPISLEQARVAVEDYVAGLGNPDLIVGEVMVFDNHAYAQIVEKSTGI
                     GALEVLVDPVTLAVYPEYGPNMMWNQKYSPMGDFGGMMGGGMMGRTWPPQTSGQMPVS
                     AGDAIQAARSYLDTNLPGTKADERATPFYGYYTLDILRDGQPVGMLSVNGYTRQVFVH
                     TWHGDFVEMSEE"
     gene            complement(4545..4784)
                     /locus_tag="AC162G_01995"
     CDS             complement(4545..4784)
                     /locus_tag="AC162G_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074616.1"
                     /translation="MEHEQVEREIVRRSALIYLPISAGAALLFLIAASAFGDYPTVAR
                     VGGMVWVGLLSLIVSMPTVTSRVKKQWRDKSLHNL"
     gene            complement(4798..>5200)
                     /locus_tag="AC162G_02000"
     CDS             complement(4798..>5200)
                     /locus_tag="AC162G_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR5074617.1"
                     /translation="ALAQADVGMAIGSGTDVAKETGHVILIKDDLRDVVVALETAKAT
                     MRKVKQNLFWAFVYNTLGIPIAAGLLYPAGQLVVSPELAAAFMAFSSVSVTLNTLLLK
                     RFTPSTRRGEGGPLGHTDSRKPQLTAAAAGK"
BASE COUNT          977 a         1688 c         1545 g          990 t
ORIGIN      
        1 ttcctcgccc gcgtcgctgc tgagcgttgc gatcttcgtc aggtcgtaat tcagatcgac
       61 cgagtgggtg ttcatcgcga tccggaagtc gattgtggct gctgatggat cgtcgaggtt
      121 gagcggagtg acctcgactc tcactgccgc ttcgtcgttg aactgcatgg cattggctgc
      181 aggctgcgcg gcactctcgg acgccgagcc agcagtgtca gccggtttcg gggtcagcct
      241 atcgggaccc ggcgcagagc tggtcgagcc gctgcacgcc gcaagaatga acccaagcgc
      301 gacgagcgac aaaatgatcc agggcttgct catgaatgac tctccatcat ttgggtatgg
      361 cgctgctctt tgcggatcgt ccgcacgatg atgatgatgc cgatcacgtt catcgccagg
      421 ccaacgagca tgaacggaat cttgtacgcg gcgaggaagg atgccgcagc cgacaaaccc
      481 aggagcggca gcaaatcggc gacgtggtgt gcgcagcagg cgaccatcgc cgcggtcgac
      541 gtgccgccgc ctgcacccat catcgccttg gacgcccgcg agccgtgccg cagcacgccg
      601 cgcaggtagg tgtacagccc gacctgtgtg ccgaagccgg ccatgatcgg aatcaccagc
      661 caggcatctt gtccgaacaa ctcgactgca tgttcccatc cctgggccag cgtcacgatc
      721 gtcatgtaca atgcgaacag cgccagcgcg ccgccgacgc ccatcaggat cggcaggcga
      781 tacttgtgcc accgcgcctc gcgcgtcgcc caggtcagta gcctgcttcg aaccgctaca
      841 gtatcaaatg cctcgttcat gctcgacttc ccttgcaaac tgccgccagt gtagcggaat
      901 agcgggctgc ctgccagcgt ggatcggcct gattgcatcc ccgccaaatg gcgggtgtca
      961 tgtgtcatgc cgctcacctg acacacgacg cccgcttaaa gaaagcctcc cgctcgcaga
     1021 acgggaggcg actgccgggg gtattcatcg agcggtgctg tcactgcaaa tcctgcttca
     1081 tcaggtcata ctcctcgcgg gtgatctcac cgcgcgcaaa gcgctgatcg agaatctcct
     1141 tcgcggttag atcgcgtccg gtcgatgacg aagtccgccc gctgttgaac agcgcctgag
     1201 ccagccacac cgccccaacg atcaacccga cccagaacag aatcatgatc aacccgccgc
     1261 ctaaacccat gccgaacatg cctcacgctc ctttccgtta ggtgttgaga tccgccagca
     1321 tctgctgata ctgctcacgc gtgatctcgc cgcgcgcata gcgaacttgc aggatgtcgc
     1381 gtggtgagac gcttgagggg gccgcttggg acgcccgccc gttcgcgtcg atgcgcctga
     1441 ccagccaggc gatcagcagc acgacgccga ggatcaggcc aatcgtgatc acccagttca
     1501 agatcatgcc gatccagcca aacgtcccga agtctccgaa tccagtcatc atggttacca
     1561 ctccttgttc agtttacgcg ctcatcatag cgaagttggt taaaggcgtc ttgaagcgaa
     1621 tttaaagatt cggtaaagat gggtatgtcg attcagttgg cgatgggaag ggtgaaggtg
     1681 aaggtgctgc cctcaccggg gcctgggctg gcggcccaca tacgcccgcc gtgcgcttcg
     1741 acgaggtgct tggcgatggt caggccgatg ccgctgccgc cgcctgcgcg agagcgggat
     1801 ttgtccaccc gatagaagcg cgtgaagacg tgcggcaggt gctcgggggg gatgccgatg
     1861 ccggtgtcgc tgacaaagat gtagatttcg ccgccgtggc gccgcgcggc cacgcgcact
     1921 ttgccctcgg ccggtgtgta ttgcagtgca ttaccggcca ggttgagcag tacctgaccg
     1981 atgcggtctt catccaccat cacagcaggc aaaccggcag gcaggtcggt ttcaagcgcg
     2041 acctgtttct cggtgaactg gcgaccgagc cgggcggcca gggtatccac taattgatgc
     2101 acctgcaccg gacgacggtt gagtgaatag gcccccgcct cgacgcggct gagctcttgc
     2161 aggtctgcta ccaggtgctg caggcggtcg gcttcgcggt acacctgttg gaacgtctcg
     2221 acctcggcgg gcagcacgcc gtcgatcagg ccctccatcg agcctttgat cgccgtcagc
     2281 ggcgtgcgca gctcgtgagc cacgtcgccg atcagttggc ggcgcatgct ctccgttcgt
     2341 tccagtttgt cggccatctg gttgaagtga agcgccagtt gggccagttc gtcgagttca
     2401 ttcttggccg ggtcgccgga tacacgcacg cgctcgccgt agtgcccacc ggcgatgcgc
     2461 tgggtggccg ccgtcatggc gcgcaccgga gcgacgacct gccggctgac gaacaggctg
     2521 accgcgacgg ctccgatgag cgctgccaat gcagccagcg tcagcgcctc gctgactgca
     2581 ccgcggaagt cggcgagcag gtcgcccatg tccatcattt gcccgcccat catcatcgag
     2641 tccatgccgg cgatatggcg ctgaaacgcg ccgggcgcag cgaagttcgc agccgtcgcc
     2701 agcactgcga cgcccacgac aatcacgatc aggtacgaca gaaaaatctt ccagcccaga
     2761 tgccgtcgaa tgtgactcgc aatcatccca ctccctcgtc ctcaaagcga tagcccacgc
     2821 ctcgcaccgt ggcgatgaac ggctcgccca acttctggcg gatgtgccca acgtgcacat
     2881 cgacgacgcg tatttcgccg taataatcgt agccccaaac tcgttccagg agctgctcgc
     2941 ggctgagcac cttgccgtga tgctcggcca gcgtcttcag cagatcgaat tcgatcgtcg
     3001 tcagttccac cggctcgtcg tccagccata cctggcgact gccggtgtcg atgcgaacgt
     3061 gccggaaagc cagcacctcg ccggatagca ggcttgcgcc ggttcgcagc cggcgcagcg
     3121 cagccttgac gcgggcaacc agctcgcgcg ggctgaacgg cttggtcagg taatcgtccg
     3181 cgccaaccga caggccgatc accttgtcgg cttcttcgga cttggccgtg agcaggatga
     3241 cgtacacgtc cgactcgcgg cgcaggtgcg atagcacttc caacccgtcc atgcccggca
     3301 gcatgatgtc caacacgatg aggtcaggcc tgaaggcgcg cgcggctttc agcgcggatg
     3361 gcccgtccat tgccgtgtac gtctcgtagc cctccttctg tagataggcg gtcgccaggt
     3421 tgaggatact ctgctcgtca tcgaccaata gaatctttgt ggaggacacg ccatactcct
     3481 caaggggaca aaggtatgac acgcattcta tccgaaaaca acgaccgggc aaagacggcc
     3541 cggtctgttc acctacgtcc ttatcagcgt ttatccgcgt tcatccgcgt cctgttactc
     3601 ttcgctcatc tcgacgaagt cgccgtgcca ggtgtgaacg aagacctgcc gggtgtaccc
     3661 gttgacgctg agcatgccta ccggctggcc atcgcgcagg atatcgagcg tgtagtagcc
     3721 gtagaacggg gtcgcgcgtt cgtcggcctt tgtaccgggc agattggtgt ccaggtagct
     3781 ccgcgcggcc tggatcgcat ccccggcgct gaccggcatc tggccggacg tctgaggagg
     3841 ccacgttctg cccatcatgc cgccgcccat cataccgccg aagtcgccca tcgggctgta
     3901 cttctggttc cacatcatgt tgggaccgta ttcgggatag acggccagcg tcaccggatc
     3961 gaccagcact tccagcgcgc cgataccggt gctcttctcg acaatctgcg cgtaagcgtg
     4021 gttgtcgaag accatcactt cgccgacgat caggtcggga ttgccgagac cggcgacgta
     4081 atcctcgaca gcgacccgcg cctgttcgag cgagatgggt tcgacgccgt acaggctgcc
     4141 gctatatccg cccatcatgc cgcggcccat catccccgat ccgcccatca tgccaccccc
     4201 catcatgcca gggccatggg gcagcgtgcc ggagtaggtc tgagcgtagc ccatcatacc
     4261 tggccccatg ccatagccgt agggaacctg ggtcgatccg gtctggtccg gatcgtaacc
     4321 gtaggagcgg cccatcatgg tcggaccata gccggcccag cccgaagcgc cgcgcgcgat
     4381 aagcacgccg ctccacacca gcgccgccgc cacgacgacg atgccgacga taatcagtgt
     4441 ccagcgtaaa gtcctgttca tggttgcctc cagtcgatct tcctttcagc ctgaattgat
     4501 ggcctcactt taaccggcct gctcaaaggt cacgtgaagg caagttaaag attgtgtaaa
     4561 gatttgtcgc gccactgctt cttgacgcgc gaggtgacgg tcggcatcga cacgatcagg
     4621 ctgagcaacc cgacccatac catgccaccc acacgcgcca ccgtcggata gtcgccgaag
     4681 gcgctcgccg cgatcagaaa cagcagcgcc gcgccggccg aaatcggcag gtagatcagc
     4741 gcggatctgc gcactatttc tcgctcgact tgttcgtgtt ccattgggcc tctcctgtca
     4801 ttttcctgca gcggccgccg ttagttgagg cttgcgcgaa tcggtgtgcc ccaacggccc
     4861 gccttcgccc cgccgcgtcg acggcgtgaa acgcttgagc aagagcgtat tgagcgtaac
     4921 cgataccgag ctgaacgcca tgaaggccgc cgccagttcg gggctgacga ccaactgacc
     4981 ggcagggtac aacagcccgg cggcaatggg gatacctagc gtgttgtaga cgaaagccca
     5041 gaacaggttc tgcttcacct tgcgcatggt ggccttggcg gtttcgagcg ccacgacgac
     5101 atcgcgcagg tcatctttga tcaggatcac gtggccggtt tctttcgcca cgtccgtgcc
     5161 tgacccgatc gccatgccga catcggcctg ggccaatgcg
//
LOCUS       DBNHXB010000055         5155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3031008, whole genome shotgun sequence.
ACCESSION   DBNHXB010000055 DBNHXB010000000
VERSION     DBNHXB010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3031008"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="AC162G_02005"
     CDS             <1..910
                     /locus_tag="AC162G_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074618.1"
                     /translation="PFDDEGLTAGGDAWHTVASLIIPPRSAPPLEQTLRQVFGGRIAV
                     LGVAPIGIVPKSSDGQARLNWSRIAEVGDGGIRVSLSLVDAAGHVVWSNVFEPYDGAF
                     PVAEWPSGNLQTVLGFHTPDQEGAYSLRLAFLDETGAPLPARCGWLEPATSGCPIGSV
                     QVEGEAISDAINFDYQVLLTEWLVDRAEMRPGETIDVQLTWRGLKEWEDDYTVTVQLI
                     GPDGRLHGQVDAWPVQGTLPTSTWKAGQTIADPYVVTLRPDAPSGRYRVEVGWYLLKT
                     LRRLPILDANGRPTDDRFVVGEFVVP"
     gene            1046..2071
                     /locus_tag="AC162G_02010"
     CDS             1046..2071
                     /locus_tag="AC162G_02010"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5074619.1"
                     /translation="MKVRIHTTAAAFDVLAPEWNTLLHGSAADTPFLTHEWQTAYWRA
                     LGEEALKIIEVRDAGGALAGLAPLFLAEAESKRSVRLVGGVDPSDYLDFILAFEREAE
                     LGSAILDALAAEEDWDRIDLYNVPEASPTRAWLAQAAATRGWTYTDERQVPSPILWLP
                     DSFDEYVARLDKRDRHELRRKLRRADAMEGLRWYVVDGEYASELEPEVDAFLDLMARS
                     HPDKAGFMTPKMRGFFYEAVRAAHRGRWLQLAFLEVEGRKAATYLSFDYGDRLMIYNS
                     GYDPDTSGGFSPGIILIARLIAQAIQGGKRAVDFMRGDEEYKYRLGAKDTWVHHLSVE
                     RRMRDEG"
     gene            2196..3470
                     /locus_tag="AC162G_02015"
     CDS             2196..3470
                     /locus_tag="AC162G_02015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5074620.1"
                     /translation="MAIHRVAMLSVHTCPLATLGGKETGGMNVYVRDLAREFSRRGIM
                     VDVFTRSQNPHLPHVMHKLGARGRVVHVPAGPEEPCDKDKVFDYLPQFTAGVLDFART
                     EGIEYDVIHSHYWLSGWVARELRAAWGKPIVHMFHTLGLMKNRVAGSASEMETGRRID
                     VETEIVGFADRLIAATPAEKCQLEWLYNTDPDCIAVIPPGVDTNHFFPIPQLAARDHI
                     GAPPDDWIILFVGRIERLKGIDTLIQAIALLAHECPPWVQHMCVAIVGGDPTTNGNAE
                     MERLKAMCGELGLSDLVAFLGARDQDQLRYYYNAASFVVMPSRYESFGMVALEAMACG
                     RPVIASEVGGLAHLVRHGETGFHVPEDDPVALASTIARLLQDEALRERLGRQAAAYAH
                     KYAWPHVADRLLAVFEPERSMPWPAFVPVKCA"
     gene            3658..3924
                     /locus_tag="AC162G_02020"
     CDS             3658..3924
                     /locus_tag="AC162G_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074621.1"
                     /translation="MKVTIDRNICTAALNACEHCFSFFAQNPMGVDRYCIIEQIDDGS
                     DELTLTIRSDNTERTIVLDDARRELVVTEGWSSLVDFIPDFYRV"
     gene            4326..4955
                     /locus_tag="AC162G_02025"
     CDS             4326..4955
                     /locus_tag="AC162G_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR5074622.1"
                     /translation="MGTWLAGWGLGMSVAAPIGPVNVATIQLGLGRGFAPACAFGLGA
                     ATIDALYCFAVYLGVAPLLVAAPWLRVVLYAVGALMLARMGWGALHRRIALASDAGRR
                     PGTAWRSYLAGVSLTAVNPATILSWLAIGGAALSAIPSGEGLALVVGIFSGSACWFAL
                     LSAGASAGRRLANERILKGVSVVCGLALLGFAAVFTWGALTESLTLFSN"
BASE COUNT          906 a         1500 c         1739 g         1010 t
ORIGIN      
        1 gcccttcgac gacgaaggct tgacggccgg cggcgatgcc tggcacacgg tcgcatccct
       61 gatcatcccg ccgcgctctg cgccgccgct cgagcaaacg ttgcgccagg tcttcggcgg
      121 ccggatcgcc gtattgggcg tcgcgccgat cggcatcgtt ccgaagtcga gcgacgggca
      181 ggcgcggctg aactggtcgc gcattgcgga ggtgggagat ggggggatca gggtatcgtt
      241 gtcgctcgtc gatgcggcag ggcatgtcgt ctggtcgaat gtgttcgagc cttacgatgg
      301 cgcgtttccc gtcgccgagt ggccttcggg aaatcttcag actgtgctcg gctttcacac
      361 cccggatcag gaaggcgctt attcgctgcg gctcgccttc ctcgacgaga caggcgcgcc
      421 gctcccggcc aggtgcggct ggcttgagcc ggcgacgagc ggctgcccga tcgggagcgt
      481 acaggtcgag ggcgaagcca ttagcgatgc gatcaacttc gactatcagg tgctgctgac
      541 ggagtggctg gtcgaccgcg ccgagatgcg gccaggcgag acgatcgacg tccagctcac
      601 ctggcgcggg ttgaaggagt gggaggatga ttacacggtc acagtgcaac tgattggccc
      661 ggatgggcga ttgcacggcc aggtcgatgc gtggcccgtg cagggcacgc tgcccacgtc
      721 gacctggaag gccgggcaaa ccatcgccga tccatacgtt gtgactttgc ggcccgatgc
      781 gccgagcggc cgttatcgcg tcgaggtcgg ctggtacctg ctgaagacgc tgcggcgttt
      841 gcctatactc gatgccaatg gccggccaac cgacgacagg tttgtcgtcg gcgagttcgt
      901 cgtcccataa gtgccgtgta gatggtataa ttctgcgcgt gactgcttca ggaacccgtt
      961 ttcggaatga aaacgggttc ctgaacgaaa tgacattgtt cgcttataat gctctcgatc
     1021 ttcgtccaca ccgcatagca acggcatgaa agtcaggatt cataccaccg ccgccgcgtt
     1081 cgacgttttg gctcccgagt ggaatacgct actgcacggc agcgcggccg acacgccgtt
     1141 cctgacgcac gagtggcaga ctgcctattg gcgggcgctg ggtgaagagg cgttgaaaat
     1201 catcgaagtg cgggatgccg gcggcgcgct ggctggcctg gcccctctct tcctggcgga
     1261 ggccgagagc aagcgatctg tgcgcctggt tggcggcgtc gatccttcgg attacctcga
     1321 cttcatcctg gcgtttgagc gtgaggcaga actcggttca gccattctcg atgcactcgc
     1381 ggcggaggag gattgggacc gcattgactt gtacaacgtg ccggaagcct cgccgacgcg
     1441 ggcgtggctg gcgcaggcgg ctgcaacccg aggctggacg tacacggacg agcgccaggt
     1501 gcccagccct attctctggt tgcccgattc gttcgacgag tatgtggccc ggctagacaa
     1561 gcgcgatcgg cacgagctgc gccgcaaact gcggcgcgcc gacgcgatgg agggcctgcg
     1621 ctggtacgtc gtcgacggcg agtacgccag cgagctggag ccggaggtcg acgcgttcct
     1681 cgacctgatg gcgcgcagcc accccgacaa ggccggcttc atgacgccga agatgcgcgg
     1741 tttcttctac gaggccgtgc gcgcggcgca tcgcggccgg tggctgcagt tggctttcct
     1801 cgaagtggag ggccgtaaag cggcgactta cctgagcttc gactacggcg accgcctgat
     1861 gatctataac tctggctacg atccagacac gtcgggcggc ttcagcccgg gcatcatcct
     1921 gattgcgcgg ctcatcgcgc aagctatcca gggaggcaag cgcgccgtcg atttcatgcg
     1981 tggcgacgag gagtacaaat accgtctcgg cgcgaaagac acctgggttc atcacctgag
     2041 cgtggaaaga aggatgaggg atgaaggatg aaattcagaa tggtgaattt agaatgatga
     2101 atgcggaatg gggaaaccag gattggcgat acgcgacagg cgataggcga tacgcgataa
     2161 gcgatacgca gcagacgata cgcaaaggat tgtctatggc gatccatcgt gtagcgatgt
     2221 tgagcgtgca cacctgcccg ctggcgacgc tggggggcaa ggagaccggc ggcatgaacg
     2281 tgtacgtgcg cgatctggcc cgcgagttct cgcggcgcgg gatcatggtc gacgtgttca
     2341 cccggtcaca gaacccgcac ctgccgcacg tcatgcacaa gctgggcgcg cgggggcgcg
     2401 tcgtccacgt gccggccggg ccggaagagc cgtgtgacaa ggacaaagtg ttcgactacc
     2461 tgccccagtt caccgcgggc gtgctggatt tcgcgcgcac ggagggcatc gaatacgacg
     2521 tgatacacag ccactactgg ctgtccggtt gggtcgcgcg ggagctgcgc gctgcctggg
     2581 gcaaacccat cgtccacatg tttcacacgc tggggctgat gaaaaaccgg gtcgcaggca
     2641 gcgcgtcgga aatggagacc ggccgccgca tcgacgtgga gacggagatc gtcggctttg
     2701 ccgatcggtt gattgcggcc acgccagccg agaaatgcca gttggaatgg ctgtacaaca
     2761 ccgatccgga ctgcatcgcc gtgatcccgc ccggcgtcga tacgaaccat tttttcccga
     2821 taccgcagtt ggcggcacgc gaccacattg gcgcgccccc cgacgactgg attatcttgt
     2881 tcgtcggccg gatcgagcgg ctgaaaggga tcgacacgct gatacaggcg atcgcgctcc
     2941 tggcgcacga atgccctccc tgggtgcagc acatgtgcgt agcgattgtg ggcggcgatc
     3001 cgacgacgaa cggaaacgcc gaaatggagc gcctgaaggc gatgtgcggg gagcttggcc
     3061 tgagcgacct ggtcgctttc ctgggcgcac gtgatcagga tcaactgcga tactactaca
     3121 acgccgccag tttcgtcgtg atgccctcgc gctacgaatc tttcgggatg gtagcgctgg
     3181 aggcgatggc gtgtgggcgg ccagtgatcg cgtcggaggt cggcgggctg gcgcatctcg
     3241 tgcgccacgg tgagacgggt tttcacgtgc cggaagacga tccggtggct ttggcatcga
     3301 ccatcgcccg cctgctgcag gatgaggcgt tgcgcgagcg cctcggccgc caggccgccg
     3361 cgtacgccca caagtacgcc tggccgcacg tggcggatcg gctgctcgcc gtgttcgagc
     3421 ccgagcggag tatgccgtgg cctgcgttcg tgccggtcaa atgtgcctga ttgcacaagt
     3481 caagtcgatt cttgaggacc ggcgcctgct gaccacgagt cacttttctc agtgtctcag
     3541 cgttcagtga tgactgtcac ggcagggatc ggccattcgt cactgtagcg cgccttcctg
     3601 tcacggtaag cttaagtcgt gtaactctgc cgtgcagagc caatcgagga ggcagccatg
     3661 aaagtgacga ttgatcgcaa catctgcacc gctgcgctga acgcctgtga gcactgcttc
     3721 agcttcttcg cgcagaaccc gatgggcgtc gatcgctatt gcatcatcga gcagatcgac
     3781 gacgggtcgg atgaactgac cttgacgatt cgcagcgaca atacggagcg gaccatcgta
     3841 ttggacgatg cccggcgcga gctggttgtc acggaaggct ggtcgtcgct ggtcgatttc
     3901 atccccgatt tctatcgcgt gtaagactca cgtaagccga tcaagccggg actgcgccac
     3961 tagccgaacg ctgattcgcc tctgggaatt atccacatct gctcattgtc cgcgaggacg
     4021 cgggccttga gcgacttgtg tgcaatcacc agattcgcct cgcttgacgg gttcgcaggc
     4081 tcctggcata atgtcgctca tgataagtat agtcaggcga agcatctctg ctggcgatgt
     4141 gcagcaggcc gcgcctgccg gtgcggcagc ggacggtctg tcgatgattg cgtgatgcgt
     4201 tgttgacctg ttgcgcggac tgcccccggc gttcttcctc gccgggggct ttttgttgcg
     4261 cggctgcccg cgatacaccc tatgcgataa gcgatacgcg ataagcgaaa agcaggaggg
     4321 cgatcatggg aacgtggcta gccggctggg gattgggcat gtcggttgca gcgccgatcg
     4381 ggccggtgaa tgtcgcgacg attcaattgg ggcttgggcg aggatttgcg ccggcctgcg
     4441 cgttcgggtt gggcgcggcg acgatcgacg cgctgtattg ctttgccgtc tacctcggcg
     4501 tcgcgccgct gctcgtcgcg gcgccgtggc tgcgcgtcgt gctatacgcc gtgggcgcgc
     4561 tcatgctcgc ccgcatgggc tggggcgcgc tgcaccgccg gatcgcgctc gcatcggacg
     4621 ccgggcgccg gcccggcact gcctggcgat cctatctcgc cggggtgagc ctgacggcgg
     4681 tcaatcccgc gacgattctg tcctggctgg ccatcggcgg cgccgcactg tcggcgattc
     4741 catccggtga gggattggcg ctggtggtcg gcattttctc gggcagcgca tgctggttcg
     4801 ccctactatc ggcgggcgcg agcgcaggca ggcggctggc caacgagcgc atcttgaaag
     4861 gcgtatccgt cgtgtgcggg ctggcgctgc tcggatttgc agcggtgttc acctggggcg
     4921 cgctgacgga gagcctgacg ttatttagca actgagtctc ggtagagtat ggatttgatt
     4981 cgtagtagcg acttcagtcg cttctgtgct ggaacgactg aagtcgtcac tacggaatcg
     5041 tgatctactg agtctcggta gatccagatt tggaagcaga gcaggagcgg cgcaaagcac
     5101 gcattccttt acgatgtgct ctgatcgcgg tacaattcgc cgcgcttgag gatca
//
LOCUS       DBNHXB010000056        18861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4540901, whole genome shotgun sequence.
ACCESSION   DBNHXB010000056 DBNHXB010000000
VERSION     DBNHXB010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 18861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18861
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4540901"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="AC162G_02030"
     CDS             complement(<1..189)
                     /locus_tag="AC162G_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HIR5074623.1"
                     /translation="MTKRKSTARRASAPKADKTKVLVLVGTRKGGFIFTSDRKRREWK
                     VSGPHFKGWTVMHLTLDPR"
     gene            354..1550
                     /locus_tag="AC162G_02035"
     CDS             354..1550
                     /locus_tag="AC162G_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HIR5074624.1"
                     /translation="MAKKKSTARRASAPKADKTKVLVFVGTRKGGFIFTSDRKRREWK
                     VSGPHFKGWTVMHLTLDPRDRRIHAATSHYVYGPNTHYSDDLGKTWTQSKESPAFPQP
                     STSGRPLGTPDEAADPEAAKSKLEKVIKPWNVTPGRTDEPGVLYAGIEPAALFKSTDR
                     GETWHIVDGLYNHPHRPTWFPGNGGLCLHTIILDPANPNRMYVGLSTGGCYRTDDGGA
                     TWQPRNKNVRADFLPDKFPEYGQCLHKMVMHPGRPDVLYQQNHCGVYRSDNAGDDWID
                     IGEGRLPSRFGFPIAVHPHDPKTIYVVLEESDDYRMSVDGKFAVWRSKNGGDLWKRLN
                     SGLPDRAHLVALREAVAVDTLDDAGVYVGANTGQVFHSRDAGDSWGLLVDFLPPVLSV
                     ETAVMR"
     gene            complement(1592..1921)
                     /locus_tag="AC162G_02040"
     CDS             complement(1592..1921)
                     /locus_tag="AC162G_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HIR5074625.1"
                     /translation="MKLILAVIQKEDEDALTDALEQSNFGATRIGSSGGFLRASNVTL
                     MIAVEDGRVSQVLELMRQHCKRRTRHLNPMLPSLEARERFLGSVPVEIGGATVFVLNL
                     EQMEKIG"
     gene            complement(1996..3237)
                     /locus_tag="AC162G_02045"
     CDS             complement(1996..3237)
                     /locus_tag="AC162G_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5074626.1"
                     /translation="MQLSRPPHRRSSPRRVVVYLALIAGGLLFLRFEGDLRRPVLPTP
                     TATRTAQSYVAEGQAHYEAGRFSAAIDAYTQAAALEPNRVDVLITLSRILALADRTGQ
                     ALQRAQRAVQIDSKSAPAHAALSMAYDWHGTWLLLHGRDVEASDHYEKAVSAALEAIQ
                     LDPNYPEARAYLAEAYMDLGRLVDAIESAQQAVEIDPKRPDVQRAVGYVRESQGNYRG
                     AAEAYTEALRTDPNVPYLWLALGRNYRILTTQTDAGYFDDAVGAFERAIQIDPNYGPA
                     HDELGWTYYNFQDYRKATEVLEEAVRVDPSSWSAHNHLGIAYYSNRNYEGAVETLPRA
                     IELMNEAFDADRYCVNATSRACDRLVEAYYILGLADFYLANCEESYRLFDKALALRPE
                     EPNALEGIRLCREAEADTSAP"
     gene            3381..5207
                     /locus_tag="AC162G_02050"
     CDS             3381..5207
                     /locus_tag="AC162G_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HIR5074627.1"
                     /translation="MRCQSCGAALPPDVRLCPNCGTLQQDTRPAPRIRSSAPDPGATA
                     GASRQIGLAIMVVTGFACALLVVLAGSGYAGYEAGKSDRATLRAQEADKYYGRGLEDL
                     GAGRLELAAADFAYVLQLDPNYPDAALQLQAARDRIAELARVEPTPTSAPAEMAATLV
                     QAAREAYDAGDLQAAIERLVQARELEPGFERQAVEDLLFRASYTHGLQMLDRDRLEEG
                     VYYLEQAAALRPLDADAALQLEYAKLYLTARGYWNVNWDRAIERFGELVNIAPGYKDT
                     YSRYVTAHILHADSYVARGDYCPAQPLYQKAISLRPDAAVQAKLEEATAGCLSATPVP
                     LTGTLPISGTPVAVPGITVGRLAYPVYDEATGSYTIYAISPGAGPFPAATGGQPAWQP
                     NGSSLAYRVLGIGVNVIDISSGLASSVAPAGSAWPTWSPDGSRMAYAQRDTAGNFQIM
                     IVGLDGASPPVILGPGASPIWGPNGVLAYSGCDASGCGILVDNPDNADPPVRLTASAG
                     DTPTSWSPDGFNIAYFSNSDGDWDIYFVNTAGGVAQVINSPDDDGMPAWSPDGAHLAF
                     ASNRDGEWAIYTVRFDGQELTKSITLGPSSPNWLNERLAWAP"
     gene            5252..5725
                     /locus_tag="AC162G_02055"
     CDS             5252..5725
                     /locus_tag="AC162G_02055"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5074628.1"
                     /translation="MEVRPIDEGDREWVGRFLAERWGAAVVVTRGKAHHADRLPGFLA
                     FQDRAPIGLITYRIDGAECEIVTLDSVREGMGVGSALIGAVKDAARRAGCTRLWLITT
                     NDNLPALRFYQRRGFSLVAVHRNAIEESRKLKPSISAIGIDGIPIRDEIELELRL"
     gene            complement(5762..7129)
                     /locus_tag="AC162G_02060"
     CDS             complement(5762..7129)
                     /locus_tag="AC162G_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5074629.1"
                     /translation="MSNDLQSRVDAYIQDHLDEWIAELSTLCAQPSVSAQNLGIRECA
                     ALVADMLRKRGFVAEIMPTAGHPVVYAEARGKSSKTLMFYNHYDVQPPEPLELWDSPP
                     FEPALREGALYARGVSDDKGHIVCRLAAIDAIKAVTGELPCNVKFLIEGEEEISSVSL
                     PPFIKANREKLAADACIWEFGGVNEAGIPMQDAGMRGICYVELRAKTAILDAHSGLGG
                     SIFPNAAWRLVWALNTLKDPDEHIRIPGFYDNVVPPTQRDLELLDALPDESQNYLKRY
                     GLKGFLKGLAGGLELKREGVFVPTCTICGLTSGYQGPGSKTVLPAEASAKVDFRLVPN
                     QTPEEIVAKLRKHLDAEGFGDIEVAFIGGEAPGRIDPDHPFLKLVVEAARGIYEHDML
                     VAPMIGGSGPNHAFIEYLNVPIATAGVGYPGSRAHAPNENMRLDLFFNGARHTARIIQ
                     MFAGG"
     gene            7524..8840
                     /gene="gntE"
                     /locus_tag="AC162G_02065"
     CDS             7524..8840
                     /gene="gntE"
                     /locus_tag="AC162G_02065"
                     /inference="COORDINATES: protein motif:HMM:NF041362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis PLP-dependent
                     transaminase GntE"
                     /protein_id="HIR5074630.1"
                     /translation="MALNRTRTRELYQRARQVFPLGVNSNFRYWGDEHTNVFVRGEKG
                     YVFDADENRFIDYRLAYGPVILGHGYPAVVERVAQAIKDGGVFAATHPLEISAGERVQ
                     RLTGMDKVRFANSGTEATMHALRIARAYTGREVVIKFEGNYHGFHDYVLFSTAMAGRG
                     TLGARRSPLNVPASSGIPKVINELLINLPFNDFEALERTVKARWGNIAAIILEPCQGN
                     SACVMPAEGWLQHIRRLCDEHGIVMIIDEVKTGFRIANGGAQEYFGVRADLATYAKSI
                     ANGFPLAAIAGKEDIMSIVGPGSVAQGGTYVGNIPGTAAADATLEILETQDVIGSIAK
                     RGKKLMGGLDRILTDEGIPHAIMGLPQMFGFVLGIDETPRDYRACLDCDLELYEHIIM
                     EMIQRGAMAEPDYREPWFLCYEHSDADIDETLNAYADAVKAVKRRH"
     gene            complement(8989..9783)
                     /locus_tag="AC162G_02070"
     CDS             complement(8989..9783)
                     /locus_tag="AC162G_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074631.1"
                     /translation="MAFHLHRDRTRALRRLALTSVLFIVATAAAVGVGRLAQARPQAT
                     TLATIFSENFEGGFGAWQSSAPPGSLGPRWNFVGSGSPYPKPAGGPTGPNAIWFGDPA
                     TGQYGGACLPDNPCPRYAGTLTYIGTPILVPSNETYVYMTFMSWELTEKSFYWGCAPG
                     DFGTPYCVNDVRQVWISGTSDSTWRLKWNTYQRPIVEQVWRSIGLIDLSEYKGQSIRI
                     RFGFDTVDGNYNPGPSPSKPAGWYVDNIQLFTFTPTDRVYLPLIRK"
     gene            complement(9833..10681)
                     /locus_tag="AC162G_02075"
     CDS             complement(9833..10681)
                     /locus_tag="AC162G_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR5074632.1"
                     /translation="MHIAVSSWSFHRSLYGGTLHLPDVPLAVYECGIRYVELNDLFLA
                     PPQPGRLARLFGTRAAQASGPDYGRASLMSVHQGRLRSGTRLACWTIETDLTAGTAEE
                     QSAQRAYIATAIEAARFLGSPIVRLTLGGESGDHSAVGRAVNLLRGVLPVAVASGMRL
                     AIENHGGLSSEAGVLEEVTGAFASIDSAGAPAIGVCLDLGHFAEGEREAGTARLAPLA
                     IHVHAPARAFDERGEETTIDYRGAMGALRTAGYDAAISIEYTGDDDAREGIRKTQALI
                     ERHWNG"
     gene            complement(10726..11577)
                     /locus_tag="AC162G_02080"
     CDS             complement(10726..11577)
                     /locus_tag="AC162G_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5074633.1"
                     /translation="MTKDVGLSSFVRRRLVECMPHLHVNGVTLNTVDEGSGDVIVFLH
                     GLGSCGEDWVFQTAFFCANYRVLAPDLRGHGRSGKPAGPYSIALFASDVAAMLDALSI
                     GRAHVVGLSLGGLVALQMALDFPDRVRSLALTNAFAHLLSVDAARLARLMWRGIISLA
                     LPLDRSAQRVARSLFPFPHQAELRRLTAARIAANDRAAYRASIAAIRRFDSRRRLHRV
                     ARPVLIVTGDRDRTVPRSLQRRLAQSIPGARWEIVRDSGHATPIDQPEAYNTLLASFF
                     RNVGERD"
     gene            complement(11595..12440)
                     /locus_tag="AC162G_02085"
     CDS             complement(11595..12440)
                     /locus_tag="AC162G_02085"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020034.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5074634.1"
                     /translation="MPQLAFVPADEYSLDQLSRLLTRAYTGYAVPIDVDAIGLEAMIA
                     AYDIDLAGSRVGVRGGQPVAIALLGVRERRGWIGGMGVAPEMRGERAGLAVTRAVIGS
                     ARRMRLKSIDLEVLTDNLPAIRIYESLGFKRRRALDVWLRDSSATFPMPPGPEVGPLE
                     VPACLAAFDELHVIAPPWQRDLPVLRRAASSLHALGTTDGGRITSYLLYRMDGPRVNI
                     LDTAAAPRRRTQAIEGLLRALIRERAGSLVRFVNLPQDDPASEAMHRVGAEVERQQHE
                     MTLDL"
     gene            complement(12440..12742)
                     /locus_tag="AC162G_02090"
     CDS             complement(12440..12742)
                     /locus_tag="AC162G_02090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR5074635.1"
                     /translation="MIVVANRIRVTPGFEAEFEQRFAERESLLRDEPGFIRNLVLRPV
                     KSDVYVVMTFWQDLAAFERWTQSESFRQSHARPTPSGMFAAPNVLEIHDVAHMVEA"
     gene            12873..13406
                     /locus_tag="AC162G_02095"
     CDS             12873..13406
                     /locus_tag="AC162G_02095"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIR5074636.1"
                     /translation="MTRVKDRPVSVIFVCSGNICRSPMAEAIFAHLVEKAGLTDRFHI
                     ASAALGDWHVGETPHRGTLDVLRRHGVPPTAGKRAQTVDAAMLARADYIIAMGGEHVR
                     ELRAVYGDVIGGKASRLLDYWSTARMRDVPDPYYTGDYQEVYDLVEPAARALLEHIRR
                     RERLEAPADSESLPRVP"
     gene            13403..14278
                     /locus_tag="AC162G_02100"
     CDS             13403..14278
                     /locus_tag="AC162G_02100"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HIR5074637.1"
                     /translation="MIPDTLRSAIAEALAVTGDSTPVRSLEPVGGGDISEAARVKTDR
                     AHYFVKWNRRPLPNVFEAEARGLRLLASARAVRVPGVIAVIERPPALVLEWIDTRGAK
                     SDASETLGRQLARQHRMLGAGYGLDHDNYIGANPQRNETAPTWIEFFRDRRLGFQMQL
                     ARRNGYLDARRERRLERLMSHLEKWIEESACAASLLHGDLWGGNFVADAAGDPVLIDP
                     AVYYGDREAEIAFTELFGGFGPRFYAAYGEAWPLAPGYAERRDLYNLYHLLNHLNLFG
                     EGYGGSVDAILRRYV"
     gene            14386..14958
                     /gene="xpt"
                     /locus_tag="AC162G_02105"
     CDS             14386..14958
                     /gene="xpt"
                     /locus_tag="AC162G_02105"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010387491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HIR5074638.1"
                     /translation="MEILKQRILAEGRNLGRGILKVDSFINHQVDPVLMHACAKELAR
                     RFQPLRASRVLTAEISGIAPALLTALELGVPIVYARKSKPITMPASVFLTTAPSHTKG
                     VDVNLMVSPEYLSAGERVLIIDDFLATGHTIMGLVRLAQAAGSTVVGIGAVIEKTFEG
                     GRDVLKSLGVPIESLAVIVDMKGGRIVFQK"
     gene            14942..16522
                     /gene="guaA"
                     /locus_tag="AC162G_02110"
     CDS             14942..16522
                     /gene="guaA"
                     /locus_tag="AC162G_02110"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR5074639.1"
                     /translation="MSSRSDAIAILDYGSQYTQLIARRVREARVYSEIFPWDAPPERV
                     MALRPRGFILSGGPNSVTDPGAPALPQYVLDSGLPVLGLCYGMQLLAHALGGRVAPSQ
                     AREYGPAMVELTDPSCELFNHLAPIHPSTNYQVWMSHGDRIESVPPGFHPVACSQNSP
                     IAAMADPARKLYALQFHPEVAHTPRGQDILRAFVLDIAGCAPGWTPASFVDEAVERIR
                     AQVGDELVIAGLSGGVDSAVAATLIHRAIGDQLTAIFVDTGMLRRGEPEQVVETCRQA
                     LGMKLVAVNAIEEFLTALGGVTDPEQKRMIVGEKFVRVFEREVRRLEQVAGHEVRFLA
                     QGTLYPDVIESRGPERRQAAKIKTHHNVGGLPPNMKLELVEPLRYLFKDEARAVGAAL
                     GLPDSIVWRQPFPGPGLAVRVIGEVTWERLETLRAADAIVREEIDRAGLSFAVAQAFA
                     VLLPVRSVGVMGDGRTYADVVAVRAVTTDDFMTADWARLPYDMLARISNRVVNEVQGV
                     NRVVYDISSKPPATIEWE"
     gene            16577..17662
                     /locus_tag="AC162G_02115"
     CDS             16577..17662
                     /locus_tag="AC162G_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074640.1"
                     /translation="MSKIIPIALAALILLGVTACGQPSLPEGHLSRALTFVPNNASEF
                     MYIDWALIKKYKDVADLTSRSDMEKRREFMLSIGKDQAGASGYALARFFQHAEAWGWD
                     STDLIWETMLMYDGAPPAYVLRLRDDFDTTAIVRRFDERGFTQSQHNGATIYSHEMDL
                     TADWLRLTEFGILNTAVVADEKTLVLSTSIDNVKAIVDAYRDQSRSSGGETAFQAIAD
                     RLGEVAAAALSRAEVVCRSLNEANISAQTEAVKAILGPARALHAYQAFGIGYRYENEK
                     PIGLLVFHYANAADAQADLDARRRLADEATSLSTRQPYSVALFTLNQALVDGSDLTLQ
                     VSPVNDLPRRLFDMVRRRDVIFAVCPQ"
     gene            17765..17926
                     /locus_tag="AC162G_02120"
     CDS             17765..17926
                     /locus_tag="AC162G_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074641.1"
                     /translation="MWRDQLGGPDAAVRWAVAEEIGGEGEWQSLQNGDAISLTRYGVL
                     IVLADGTWK"
     gene            17923..18633
                     /locus_tag="AC162G_02125"
     CDS             17923..18633
                     /locus_tag="AC162G_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074642.1"
                     /translation="MSLRAIIRWMSVAGLVIGAACAPPPALTQPTATVTPTRPPTWTP
                     TPGQSDTETPTATPHPTRTLVPPATPAEHKVVVAQDNAFSIQVPSNWEVTEGTRRVEG
                     RTNYEVKYFAAVGPGDPPQPGVIIFYEWPSSLSITNDNAWEVTNDNAWESAYALTALV
                     IKSCATQLGQEKLPVDFGGEPAFGAEYVDACGAAGLVAGAVHNGVNYGALFEAPAPYF
                     GEWFDTLREILLSITFGR"
BASE COUNT         3441 a         6158 c         5977 g         3285 t
ORIGIN      
        1 gcgcggatcg agcgttagat gcatcacggt ccagcccttg aagtgcggcc cggagacctt
       61 ccactctctg cgcttgcggt cgctggtgaa gatgaagcca cccttgcggg tgccgacgag
      121 gaccaaaacc ttcgttttgt cggccttcgg cgcggaagcg cgccttgccg tcgacttcct
      181 cttggtcatg gtgtccatcc cctttggttt agtggtgtca gggtaattct aatcgaattc
      241 gtgagggctg taatccccct ggacaggggc atgcccttgt ccagcgggat tacagccctc
      301 gcgaattcga ttacaattac cctgacacca ctaaaccaaa ggggatggac accatggcca
      361 agaagaagtc gacggcaagg cgcgcttccg cgccaaaggc cgacaaaacg aaggttttgg
      421 tcttcgtcgg cacccgcaag ggtggcttca tcttcaccag cgaccgcaag cgcagagagt
      481 ggaaggtctc cgggccgcac ttcaagggct ggaccgtgat gcatctaacg ctcgatccgc
      541 gcgacagacg gattcatgca gccacctcgc attacgtgta cggtccgaat acccattact
      601 ccgacgatct gggcaagacg tggacacagt cgaaagagtc gccggctttc cctcagccgt
      661 ccacgtctgg ccggccgctc ggcacgccgg acgaagcggc cgatcctgag gcggcgaagt
      721 cgaagctgga aaaggttatc aagccgtgga acgtcacacc cggccggacg gacgagccgg
      781 gcgtcctgta cgcgggaatc gagcctgccg cactgttcaa atcgactgat cgcggcgaga
      841 catggcacat cgtcgacggt ctgtacaacc acccgcaccg gccgacgtgg tttcccggta
      901 acggcggatt gtgtctgcac acgatcatcc tcgatccggc caatcccaat cggatgtacg
      961 tgggcctctc gaccggcggc tgctatcgca cggacgacgg cggcgcgacg tggcagccac
     1021 gcaacaagaa cgtgcgcgcc gatttcctgc cggacaagtt ccccgaatat gggcagtgtc
     1081 tccacaaaat ggtgatgcat ccgggccgcc cggacgtgct ctaccagcag aatcactgcg
     1141 gtgtctatcg cagtgataac gcgggagacg actggataga catcggcgaa ggcaggctgc
     1201 ccagccgctt cgggttcccg atcgccgtcc acccgcacga ccctaagacg atctacgtcg
     1261 tgctggagga aagcgacgac tatcggatga gcgtggacgg caaattcgcg gtatggcgca
     1321 gcaagaacgg cggcgacttg tggaagcgtc tgaacagcgg cctgccggat cgcgcccact
     1381 tggtggcgct gcgagaggcc gtggcggtcg atacactgga cgacgcggga gtgtacgtcg
     1441 gcgccaatac cgggcaggtg ttccacagcc gggacgcagg cgactcgtgg gggctgctgg
     1501 tcgacttcct gccgccggtg ttgtcggttg agacggcggt gatgagataa tacgagacct
     1561 ggcaggtctg agagacctgc caggtcttct cttacccgat cttttccatt tgctccagat
     1621 tcaggacgaa caccgtcgct ccgccgatct cgaccggcac cgagccgaga aaccgctcgc
     1681 gcgcctccag gctgggcagc atggggttga ggtggcgcgt tcgccgcttg caatgctggc
     1741 gcatcagctc cagcacctgg ctcacccgcc cgtcctcgac ggcaatcatc agagtgacgt
     1801 tgctggcccg gagaaatccg ccgctcgagc cgattcgcgt ggctccaaag ttgctctgct
     1861 ccagagcgtc ggtcaatgca tcttcgtcct ctttttggat cacggccagg atcagcttca
     1921 tggtctcctc acccccaccc cttgccccac ccccactcca gcgcaagagc gcaggagagg
     1981 gggaggggtg aagtgttacg gcgccgatgt gtccgcttcc gcctcgcggc acagacgaat
     2041 cccctccagc gcgttaggct cctccggtcg cagcgccagc gccttgtcga ataggcggta
     2101 cgattcctcg cagttggcca ggtagaagtc ggccagtccc agtatataat atgcctcgac
     2161 caaccgatcg catgcgcggc tggtcgcgtt gacacagtac cgatcggcgt cgaaagcctc
     2221 attcatcagc tcgatcgcgc gcggcagcgt ctcgactgcg ccttcgtagt tgcgattcga
     2281 gtaatacgcg atgccgaggt gattgtgcgc cgaccacgag ctgggatcga cgcgcacggc
     2341 ttcttccagc acttccgtgg cttttcgata gtcctggaag ttgtaatacg tccagcccag
     2401 ctcgtcgtgc gccgggccgt aattcggatc gatctgaatc gcccgctcga aggcgccgac
     2461 cgcatcgtcg aagtacccgg cgtccgtctg ggtggtcagg atgcgatagt tgcgccccag
     2521 cgccagccac aggtacggca cattcggatc ggtgcgcagc gcctccgtgt acgcctcggc
     2581 agcgccgcga tagttgccct gcgactcgcg cacgtagccg acggcgcgct gcacgtcggg
     2641 ccgtttgggg tcgatctcga ccgcctgctg cgcggattcg atcgcgtcga cgagccgccc
     2701 caggtccatg tacgcctcgg ccaggtaggc gcgcgcttcg ggataattgg gatcgagctg
     2761 aatcgcttcc agcgcggccg acaccgcctt ttcgtagtga tcgctcgcct cgacgtcacg
     2821 cccatgcagc agcagccacg tgccgtgcca gtcgtatgcc atcgaaagag cggcgtgcgc
     2881 gggcgccgat ttcgaatcga tctgtaccgc ccgctgcgcg cgctgcaggg cctggcccgt
     2941 gcggtcggcc agcgccagga tacgcgacag cgtgatcagc acgtcgacac gattcggctc
     3001 aagcgcagcg gcttgagtat aggcgtcgat cgccgcactg aaccgccccg cctcatagtg
     3061 ggcctggccc tcggcaacgt aggactgagc ggtccgggtg gcggtcggcg tcggcagtac
     3121 cgggcgccgc aaatcgcctt caaagcgcag aaacaacagc ccgcccgcga tcagcgccag
     3181 atagacaacg acgcggcgcg gcgacgaccg gcggtgcggt ggacgactga gctgcatggg
     3241 aactccggag aatgagccgt gtaaatcgat cttagcatgc ggatagggga gcgtcaaaca
     3301 gtcccgatgc tggacattcg ccgcctctcc ggctataatc gcgccatccc tcatccggtg
     3361 ctatccacgg cgatcctcgc atgagatgtc aatcttgcgg cgcggcgctt ccgcccgacg
     3421 tgcggctgtg tcccaactgc ggcacgcttc agcaagacac acggcctgcg ccacgcattc
     3481 gatcgagtgc gccagaccca ggcgcgacgg caggcgcttc acggcagatt gggctggcga
     3541 tcatggttgt caccggattc gcttgcgctt tgctcgttgt actggcaggc tcaggctacg
     3601 ccggctacga ggccggaaaa tccgatcgtg cgacgttgcg cgcccaggag gccgacaagt
     3661 attacgggcg gggtctcgaa gacctcggcg ccggccggct cgaactggcc gcagccgact
     3721 tcgcctacgt tctccagctt gatccgaact accccgacgc cgcgttgcag ctccaggccg
     3781 cgcgcgaccg catcgccgag cttgcgcgcg tcgagcctac acccacgagc gcgccggccg
     3841 agatggccgc tacgctggtc caggctgcac gcgaagctta tgacgcgggc gacctgcaag
     3901 ccgccatcga gcgcctcgtg caggcgcgcg agctggagcc tggcttcgag cggcaggctg
     3961 tcgaagacct gcttttccgc gcgtcctaca cgcacggtct gcagatgctc gatagggatc
     4021 gcctggaaga aggagtctat tacctggagc aagccgccgc actgcgcccg ctcgacgcag
     4081 atgctgcact gcagctcgag tacgccaagc tctacctgac cgcgcgcggc tactggaacg
     4141 tcaactggga tcgagccatc gagcgtttcg gtgagctggt caacatcgcg cccggctaca
     4201 aggacaccta cagccgctac gtgaccgctc atatcctgca cgcggattcc tacgtcgcgc
     4261 gcggcgacta ctgcccggct cagccgctgt accagaaggc gatctcactg cgccccgacg
     4321 cggccgtgca agccaaactg gaagaagcga cggcgggctg tctatccgcc acgccggttc
     4381 cactcaccgg gacgctgccg atcagcggca cacccgtcgc cgtgcccggc atcaccgtcg
     4441 ggcggctcgc ctatcctgtg tatgacgaag cgaccggctc gtataccatc tacgcgatca
     4501 gccctggcgc cggccctttt cccgccgcga cgggcggcca gccggcgtgg caacccaacg
     4561 gctcgtcgct cgcctaccgc gtcctcggca tcggcgtcaa cgtgatcgac atttcgagcg
     4621 gtctggcttc gtccgtagcc ccggccggat ccgcatggcc gacgtggtct cctgacggct
     4681 cgcgcatggc ctacgctcag cgcgacaccg ccggcaattt ccagattatg attgtgggac
     4741 tcgacggtgc gtcgccgcct gtcatactcg gtcccggcgc gtcgccgatc tggggaccga
     4801 acggcgtcct cgcctattcc ggctgcgatg cttccggctg cgggatattg gtcgacaacc
     4861 cggataacgc cgatcctcct gtccgcctga ccgcgtccgc cggcgacaca cccacctcgt
     4921 ggtcgcccga cgggttcaac atcgcgtact tttccaattc cgacggagac tgggatatct
     4981 acttcgtgaa tacggcgggc ggcgtggcgc aggtgatcaa ttcgccggac gacgacggca
     5041 tgcctgcctg gtcacccgac ggcgcgcatc ttgccttcgc ctccaaccgc gacggcgaat
     5101 gggcgatcta caccgtgcgc ttcgacggcc aggagctgac gaagtctatc acgctcggcc
     5161 cctcaagccc caactggctg aacgagcggc tggcgtgggc gccgtgagtg aggggattgg
     5221 aggttagaga ttggagattg gagattggag gttggaggtt cgtccgatag acgagggtga
     5281 tcgggaatgg gtgggtcgct ttcttgcgga gcgttggggc gcggcggtgg tcgtcactcg
     5341 tggcaaggcg caccacgccg atcgcctgcc gggatttctg gcctttcaag atcgcgcgcc
     5401 gatcggattg atcacgtacc gaatcgacgg cgccgagtgc gagatcgtca ccctcgacag
     5461 cgtcagggaa ggcatgggcg tcggttcggc actgatcggg gccgtcaaag acgccgcgcg
     5521 gcgggccggc tgcacgcgcc tgtggctgat cacgacgaac gacaacctgc cggcgctgcg
     5581 cttttatcaa aggcgcggct tctcgctcgt cgccgtgcat cgtaacgcga tcgaagaatc
     5641 gcgcaagctc aagccgtcga tttcggcgat cggaatcgac ggcattccga tccgagacga
     5701 gatcgagctg gagctgcggt tgtaaaactt caaactccag cccgctatta gccataagcc
     5761 atcagccacc agcgaacatc tgaataatcc gcgccgtgtg tcgcgcgccg ttgaagaaaa
     5821 gatccagccg catgttctcg ttcggcgcgt gcgcgcgtga gccgggatag ccgacgcccg
     5881 ccgtcgcgat cggcacgttc aggtactcga tgaaggcgtg gttcgggcct gagccgccga
     5941 tcatcggcgc gaccagcatg tcgtgctcgt agatgcccct ggctgcctcg acgacgagct
     6001 tgaggaacgg atgatcggga tcgatgcggc ccggcgcttc accgccgatg aatgcaacct
     6061 caatgtcgcc gaacccttcg gcgtccaggt gcttgcgcag cttcgcaacg atctcctcgg
     6121 gcgtctggtt tggcaccaat cgaaaatcga ccttggcgct cgcctcggcg ggcagcacgg
     6181 tcttggagcc aggtccttgg taaccggagg ttaagccgca aatcgtgcag gttgggacga
     6241 acaccccttc gcgtttgagc tccagtccgc cagccaggcc cttcagaaag cctttcaggc
     6301 cgtagcgctt gagatagttc tgcgactcgt cgggcagcgc gtccagcagt tcgagatcgc
     6361 gctgcgtcgg cgggacgacg ttgtcgtaga agccggggat gcgaatgtgc tcgtccgggt
     6421 ctttgagcgt attgagcgcc cagaccagac gccacgccgc attaggaaag atcgatcccc
     6481 ccaacccgga gtgcgcgtcg aggatggccg tcttcgcgcg cagctcgacg tagcagatgc
     6541 cgcgcatgcc ggcatcctgc atggggatgc ccgcttcgtt cacgccaccg aactcccaga
     6601 tacacgcgtc agccgcgagc ttctcccggt tcgccttgat gaacggcggc agagagacgc
     6661 tgctgatctc ctcttcgccc tcgatgagga acttgacgtt gcacggcagc tcgcctgtca
     6721 cggccttgat cgcgtcgatc gcggccaggc ggcacacgat gtgccctttg tcgtcgctca
     6781 cgcctcgggc gtacagcgct ccctcgcgca gcgccggctc gaacggcggc gaatcccaca
     6841 gctctagcgg ctccggcggc tgcacgtcgt aatggttgta gaacatcagc gtcttgctcg
     6901 atttgcctct ggcctcggcg tatacgacgg gatggccagc cgtgggcatg atctcggcga
     6961 cgaagccgcg tttgcggagc atgtcggcga caagcgcggc gcactcccgg atgccgagat
     7021 tctgcgccga cacactgggc tgcgcgcaca gcgtcgacag ctcggcaatc cattcgtcaa
     7081 gatgatcctg gatgtaggcg tcgactcgtg actggagatc gttggacatg cgctgttcct
     7141 ttccgtgtga ggtgacgcca attgtagacg aatcgggtca ggaggcaaac gacatattga
     7201 cgtcctggtg tatacttatc atatgcggcg cgcacgacac tggctgccct cataccatcc
     7261 tcgcggcgat cagccgttta ttccctggac ggttcgcaca ttcctgccca tcgatcgacc
     7321 ctacgtccat ccggactacc actgggttca atgggccacc ggcgactgct gagtctcccg
     7381 tgcgtctttt ccctcgccca actggttgtt gactaccgaa tttattcaac ttgcacatgg
     7441 gcagccgcaa gggcagcccc tacagctcgc ccaaccgggt attgactata gtatccattc
     7501 aatctagctc aggaggctca gccatggcac tcaatcgcac ccgcacgcga gaactatacc
     7561 agcgcgcgcg ccaggtcttt ccactcggcg tcaactcgaa ctttcgctac tggggcgacg
     7621 agcataccaa cgtcttcgtc cggggtgaaa agggctacgt gttcgacgcc gacgagaatc
     7681 gcttcatcga ctatcgcctg gcctacgggc cggtcatcct cggccacggc tacccggccg
     7741 tcgtcgagcg tgtggcgcag gcgatcaagg acggcggcgt gttcgccgcg acgcacccgc
     7801 tggagatcag cgccggcgag cgcgtgcagc gcctgaccgg catggacaaa gttcgtttcg
     7861 ccaactccgg caccgaagcg acgatgcacg cgctgcgcat cgcccgcgcc tacaccggcc
     7921 gcgaggtcgt catcaagttc gagggcaact accacggctt ccacgactac gtgctgttct
     7981 cgacggcgat ggccgggcgg ggcacgctgg gcgcccggcg cagcccgctc aacgtgcccg
     8041 ccagcagcgg cattcccaag gtcatcaacg agctgctgat caatctgccc ttcaacgatt
     8101 tcgaagcgct cgagcgcacg gtgaaggcca ggtggggcaa catcgccgcg atcatcctcg
     8161 agccgtgcca gggcaacagc gcctgcgtca tgcccgccga gggctggctg cagcacatcc
     8221 ggcgactgtg cgatgagcac ggcatcgtca tgatcatcga cgaggtgaag accggcttcc
     8281 gcatcgccaa cggcggagcg caggagtact tcggcgtgcg ggccgatctg gcgacctacg
     8341 ccaagtcgat cgccaacggc ttcccgctgg ctgccatcgc cggaaaagag gacatcatga
     8401 gcatcgtcgg ccctggctcg gtcgcgcagg gcggcacgta tgtaggcaac attccgggca
     8461 ccgccgccgc cgatgccacg ctggagattc tcgaaacgca ggacgtgatc ggcagcatcg
     8521 ccaaacgtgg aaagaagctg atgggcggcc tcgaccgcat cctgaccgac gagggtatcc
     8581 cgcacgcgat catgggcctg ccgcagatgt tcggtttcgt gctggggatc gacgagacgc
     8641 cgcgcgacta ccgcgcctgt ctcgactgcg acctggagct gtacgagcac atcatcatgg
     8701 agatgatcca gcgcggcgcg atggccgagc cggactaccg cgagccgtgg ttcctgtgct
     8761 acgagcattc cgacgcagac atcgacgaga cgctgaacgc gtacgccgac gccgtcaagg
     8821 cggtcaagcg caggcactag ccggcaccgg atccggacgg ttccgtcttc gagggaactg
     8881 agggaaccga aggaactggc ctgaagaccc cacttcggcg atttgccgga ttccagtccg
     8941 taaagagcgc gcgaggtccc cgatgtctga cctgatcggg gacttcgctt acttgcggat
     9001 cagaggcagg taaacgcgat ccgtcggcgt gaaagtaaac aactgtatat tgtcaacgta
     9061 ccatcctgcc ggtttacttg gggaagggcc gggattgtag ttcccgtcta cggtgtcgaa
     9121 cccaaatcgg atacgtatac tctggccttt atactcgctc agatcgatca ggccaatcga
     9181 acgccagacc tgctcgacga tcggccgctg ataagtattc cacttcaacc tccatgtgga
     9241 gtcacttgtg cccgaaatcc acacttgccg tacatcgttt acgcagtagg gtgtaccgaa
     9301 atctccgggg gcgcagcccc aatagaacga cttctcggta agctcccagc tcataaaggt
     9361 catatacacg tacgtttcgt tagacggaac cagaatcggc gttccgatgt aagtcagagt
     9421 gccggcatat ctggggcacg gattgtcggg aaggcaggca cctccgtact ggccggtggc
     9481 tggatcgccg aaccaaatcg cattcggtcc tgtagggcct ccggccggct tggggtacgg
     9541 gctgccgctc cctacgaagt tccaccgcgg gccgagcgat cctggcggtg ctgacgattg
     9601 ccaggcgccg aagccgccct cgaagttttc ggagaaaata gtggcaagcg tcgtagcctg
     9661 aggccgggcc tgggccaggc gccctacccc gaccgcggcc gccgttgcga caatgaacag
     9721 cacactcgtc agcgccaatc gacgcaacgc ccgagtccga tcgcggtgca gatgaaatgc
     9781 catcactcac cctcctttca cgggtcgcgg ccctgtcttc acacgtgccc gctcaaccgt
     9841 tccaatgcct ctcgatgagc gcctgcgtct tgcgaatgcc ctcgcgcgca tcgtcgtcgc
     9901 cggtatactc gatgctgatc gccgcatcgt agcctgccgt ccgcagcgcc cccatcgcgc
     9961 cgcgatagtc gatcgtcgtt tcctctccgc gctcgtcgaa cgcgcgcgcc ggtgcgtgaa
    10021 cgtggatcgc gagcggggcc agtcgggccg tgcccgcctc acgctcgccc tcggcgaagt
    10081 gacccaggtc gaggcacacg ccgatggcgg gcgcgccggc gctgtcgatg gacgcaaacg
    10141 cgccggtcac ctcttccagc acacccgctt cgctgctcaa accgccgtga ttctcaatcg
    10201 ccaggcgcat gcccgacgcg acggccaccg gcagcacgcc gcgcaacagg ttgacggcgc
    10261 ggcccaccgc agagtggtcg ccgctctcgc cgcccagcgt caggcgcacg atgggcgagc
    10321 cgaggaatcg cgctgcctcg atcgccgtcg cgatgtaggc gcgctgcgcc gattgctctt
    10381 cagccgttcc ggcggtcagg tcggtttcga tcgtccagca cgcgagccgc gtgccgctgc
    10441 gcagccggcc ctggtgcacg ctcattagcg atgcgcgccc atagtcgggg ccgctcgcct
    10501 gcgccgctcg cgttccaaac agccgcgcca gcctgcccgg ctgcggcggg gcgagaaaca
    10561 gatcgttcag ctcgacgtac cggatgccgc actcgtacac ggcaagcgga acgtcgggca
    10621 ggtgcagcgt cccgccgtac agggagcggt gaaacgacca tgaggaaacg gcgatgtgca
    10681 ttcaatatcc tccgggcggg ggcaaacgtc aatagcccga tcctgctagt cgcgctcgcc
    10741 gacatttctg aagaacgacg cgagcaaggt gttgtaggct tcaggctgat cgatcggcgt
    10801 cgcatgtccc gaatcgcgga cgatctccca acgcgcaccg gggatgctct gcgccagacg
    10861 tctttggagg ctgcgcggaa ccgtgcgatc gcgatcgccc gtgacgatca gcacgggcct
    10921 ggcaacccga tggagacggc ggcgcgaatc gaagcgccgg atcgcggcga tcgacgcgcg
    10981 atacgctgcc cgatcgtttg ccgcgatgcg cgccgcggtc aagcggcgaa gctccgcttg
    11041 atgcggaaat gggaacaggc tcctcgccac gcgctgcgcg ctgcggtcga gcggcagcgc
    11101 cagcgagatg atgccgcgcc acatcagccg agccaaccgc gctgcgtcca cggacagcag
    11161 gtgcgcgaac gcgttggtca gcgccaggct gcgcacccgg tcaggaaagt cgagcgccat
    11221 ctgcagggcg accagcccgc ccagcgacag ccccacgacg tgcgcgcgac cgatcgatag
    11281 tgcgtcgagc atggcggcca cgtccgacgc aaacagcgca atcgagtacg gcccggcggg
    11341 cttgcccgaa cggccatgcc cgcgcaggtc cggcgcgagg acgcgataat tcgcgcagaa
    11401 gaacgctgtc tgaaagaccc aatcctcgcc gcacgatccc agcccgtgca agaagacgat
    11461 cacgtccccg ctgccttcat cgacggtgtt cagcgtgacg ccgttgacgt gtaggtgggg
    11521 catacattca accaggcgac gacggacgaa cgacgacaga ccaacgtctt tcgtcattgg
    11581 tcattcgtcg ttcgtcacag gtcgagcgtc atctcgtgct gctgccgctc cacttctgcg
    11641 ccgacccggt gcatggcctc ggacgccgga tcgtcctgag ggagattcac aaagcgaacc
    11701 aggctgcccg ctcgctcgcg gatgagggcg cgcagcagcc cttcgatcgc ttgcgtgcgc
    11761 cgtcggggcg ccgcagccgt gtcgagtata ttgactcggg ggccatccat gcgatacaac
    11821 aaataagagg tgatgcggcc gccgtccgtg gttcccagtg cgtgcaagga cgaggccgcg
    11881 cgcctcagca caggcagatc gcgctgccag gggggcgcga tcacgtgcag ctcgtcgaac
    11941 gcagccaggc aggccggaac ctccaacggc ccgacttcag gcccgggtgg catggggaac
    12001 gtcgcggagg aatcgcgcag ccagacgtcc aaagcccgcc ggcgtttgaa gcccagcgac
    12061 tcatagatgc ggatggcggg aaggttgtct gtgagcactt cgaggtcgat cgacttcagg
    12121 cgcatgcgcc gcgccgaccc gatgaccgcc cgtgtcaccg ccaggccggc gcgctcgccg
    12181 cgcatctcgg gcgccacgcc catcccgccg atccagccgc gccgctcgcg cacgccgagc
    12241 agcgcaatcg cgaccggctg gccgccccgc acgccgactc gcgatccggc caggtcgatg
    12301 tcgtacgccg cgatcatcgc ctccagaccg atcgcgtcga cgtcgatggg cacagcgtag
    12361 ccggtatagg cgcgcgtcag gagccggctc agttgatcga gcgagtattc atcggcggga
    12421 acgaaggcga gctgcggcat tacgcttcaa ccatgtgcgc gacgtcatgt atttcgagca
    12481 cgttcggcgc ggcaaacatg ccggacggcg tgggccgtgc gtgcgactgc ctgaacgatt
    12541 cgctctgagt ccagcgttcg aacgcggcca agtcctgcca gaaggtcatg accacgtaca
    12601 cgtcgctctt gaccgggcgc agcacgagat tgcggatgaa tccaggctca tcgcgcagga
    12661 gcgattcgcg ctcggcgaaa cgctgctcga actcggcctc gaaacccggg gtgacacgga
    12721 tgcggttggc gacgacgatc atgtgcgcga tctccagtca gggcaatgaa tcagcaacga
    12781 tattagcatt ttcacggcgg cttggcaaac acgggcatgc ttgacaaact cggcatccac
    12841 cggaacaata ttggggctaa acggagttgg gtgtgactcg tgtgaaagat aggccggtca
    12901 gtgtcatctt tgtctgctca ggcaacatct gccgatcgcc gatggcggaa gcgatattcg
    12961 cgcacctcgt cgaaaaggcc ggcctgaccg atcgattcca tatcgcgtcc gccgccctgg
    13021 gcgactggca cgtcggcgag acgccgcatc gcgggacgct cgacgtgctg cgccggcacg
    13081 gcgtccctcc caccgccggc aagcgcgccc agaccgtcga tgcggccatg ctcgcgcgcg
    13141 ccgattacat catcgccatg ggcggcgagc acgtgcgcga gctgcgcgcc gtgtatggcg
    13201 acgtgatagg cggaaaagcg tcgcgtctgc tcgactactg gtccaccgct cggatgcgcg
    13261 acgtgcccga tccctactat acaggcgact accaggaggt ctacgacctc gtcgagccgg
    13321 cggcgcgcgc gttgctcgag cacattcgca ggcgcgagag actcgaagcg ccggctgact
    13381 ccgagagcct tccgcgcgtt ccatgatccc tgacacgctg cgcagtgcaa tcgccgaggc
    13441 attggcggtc accggcgaca gcacgcccgt ccgctcgctc gagcccgtcg ggggcggaga
    13501 catcagcgag gccgcccgcg tcaagacgga tcgagcgcac tatttcgtca agtggaaccg
    13561 gcgcccgctc cccaacgtgt ttgaggccga agcgcgcggt ctgcgcctcc tggcgtcggc
    13621 gcgcgccgtg cgcgtgcctg gcgtgatcgc cgtcatcgag cggccgcccg cgcttgtctt
    13681 agagtggatc gacacacgcg gcgcgaagtc agatgccagc gaaacattgg ggcggcaact
    13741 cgcgcggcag catcgcatgc tcggcgccgg ctacggcctg gaccacgaca attacatcgg
    13801 ggccaatccc cagcgcaacg agacggcgcc gacgtggatc gagttctttc gcgatcggcg
    13861 gctcggcttc cagatgcagc tcgcgcgccg caacggctac ctcgacgccc ggcgtgagcg
    13921 ccggctcgag cgcctgatgt cgcacctcga aaaatggatc gaggaatcgg cgtgcgccgc
    13981 ctcgttactg cacggcgacc tgtggggcgg caatttcgtc gccgatgcgg cgggcgaccc
    14041 ggtgctgatc gaccccgccg tgtattacgg cgatcgcgaa gccgagatag ccttcaccga
    14101 gctgttcggc ggcttcggcc cgcgcttcta tgccgcttac ggcgaagcct ggccgctcgc
    14161 gccgggctac gccgaacggc gcgacctgta caacctgtat cacctgctca atcacttgaa
    14221 cctgttcggc gaaggctacg gcggtagcgt cgatgccatt ctgcgaaggt atgtgtgatg
    14281 agaacgtgcg aggatacgtg attacgtgac tacgtgatta cgtattacgc ctttacgttt
    14341 tacgcttttt cgatacaatg tcatcatccc ctatggaggc tgtgtgtgga gatactcaag
    14401 caacgaatcc tcgccgaggg acgcaacctg gggcgcggca ttctgaaagt cgattcgttc
    14461 atcaaccatc aggtcgatcc cgtgctcatg catgcctgtg cgaaagagct ggcacggcga
    14521 ttccagccgc tgcgcgccag ccgggtgctg accgccgaaa tcagcggcat cgctcccgcg
    14581 ctgctcaccg cgctcgagct cggcgtgccg atcgtgtacg cccgtaagag caagccgatc
    14641 accatgccgg catcggtctt cctgacgacg gcgccttcgc acacgaaagg cgtcgacgtc
    14701 aacctgatgg tctcgccgga atatctgtcg gcgggagaac gcgtgctgat catcgacgac
    14761 ttcctcgcca cgggccatac gattatgggt ctggttcgcc tggcgcaggc tgccggctcg
    14821 accgtcgtcg gcatcggcgc ggtcatcgag aagactttcg aaggcgggcg agacgtgctg
    14881 aagtcgctgg gcgtaccgat cgagtcgcta gccgtgattg tcgacatgaa ggggggcagg
    14941 attgtcttcc agaagtgacg cgatcgcgat tctcgactac gggtcgcaat acacgcagct
    15001 catcgcgcgg cgcgtccgcg aagcgcgcgt gtactccgag atcttcccgt gggacgcgcc
    15061 gcccgagcgc gtcatggcgc tgcgcccgcg cgggttcatt ctgtctggcg ggccgaacag
    15121 cgtgaccgat cccggcgcgc cggctctgcc gcagtatgtc ctcgattcgg ggctgccggt
    15181 gctcggactg tgctacggca tgcaactgct cgcgcacgcg ctcggcggac gtgttgcgcc
    15241 ttcacaggcg cgcgagtatg gcccagcgat ggtcgaactg accgatccct cgtgcgaact
    15301 cttcaatcac cttgcgccaa tccacccatc taccaattac caagtctgga tgtctcacgg
    15361 cgatcggatc gagtccgtgc cgcccggctt ccacccggtc gcctgcagcc agaactcacc
    15421 catcgccgcc atggccgatc ccgcgcgcaa gctgtacgcg ctgcagtttc atcccgaagt
    15481 cgcgcacacg ccgcgcgggc aagacattct ccgggcgttc gtgctcgaca tcgcgggctg
    15541 cgcgccgggg tggacgccgg catcattcgt cgacgaagca gtcgagcgca tccgggcaca
    15601 ggtcggcgat gagctggtca tcgcgggcct gtcgggcggc gtcgactccg ccgtcgcggc
    15661 gacgctgatc caccgcgcca taggcgacca gctcaccgcc atcttcgtcg ataccggaat
    15721 gctgcgacgg ggagaacccg agcaagtcgt cgagacatgc cgccaggcgc tcggcatgaa
    15781 gctcgtcgcc gtcaacgcga tcgaagaatt cctcaccgcg ctcggaggcg tgaccgatcc
    15841 ggagcagaag cggatgatcg tcggcgagaa attcgtccgg gtcttcgagc gcgaagtaag
    15901 gcgactcgaa caggtggcgg gacacgaagt ccgctttctg gcacagggaa cgctgtaccc
    15961 cgacgtgatc gaatcgcgcg ggccggagcg ccggcaagcc gcgaagatca agacacacca
    16021 caacgtcggc gggctgccac ctaacatgaa gctcgagctg gtcgagccgc tgcgctacct
    16081 gttcaaggac gaggcgcgcg ccgtcggcgc ggcgctggga ctgccggact ccatcgtctg
    16141 gcgccagccg ttccccggcc cgggcctggc cgtgcgggtg atcggcgagg tcacgtggga
    16201 gcggctcgag acgctgcgcg ccgccgacgc catcgtgcgc gaggaaatcg atcgcgccgg
    16261 tctgagcttc gcggtcgcgc aggcctttgc cgtgctgctg ccggtgcgca gcgtcggcgt
    16321 gatgggcgac gggcgcacgt acgccgatgt cgtcgccgtg cgcgcggtga ccaccgacga
    16381 tttcatgacg gccgattggg cgcgcctacc ctacgacatg ctggcgcgca tcagcaatcg
    16441 cgtcgtcaac gaagtgcagg gcgtcaaccg cgtcgtgtac gacatctcgt ccaagccgcc
    16501 ggcgacgatc gaatgggagt gaggggccat ctggacattg gggaataacg acagcatcgc
    16561 gaacaaggag gctcccatgt cgaagatcat accgattgcg ctggccgccc tcatcctgct
    16621 cggcgtgacc gcgtgtgggc agccctcact ccccgaaggc catctctctc gcgcgttgac
    16681 gttcgtcccg aacaacgctt ccgagttcat gtacattgac tgggcgctca tcaagaagta
    16741 caaggatgtc gccgacctga ccagtcgcag cgacatggag aagcgcaggg agttcatgtt
    16801 gtctatcggc aaggatcagg ctggagcgtc cggctatgcg cttgcgcgat tcttccagca
    16861 cgccgaagcg tggggctggg acagcaccga cctgatctgg gagacgatgc tgatgtacga
    16921 cggcgcgccg ccggcgtacg tccttcgcct ccgcgatgat ttcgacacga cggcgatcgt
    16981 gaggcgattc gacgagcgcg gctttacgca gagccagcac aacggcgcga cgatctattc
    17041 gcacgagatg gacctcacgg cggactggct gcgactgacc gagttcggca tcctcaacac
    17101 cgccgtcgtg gccgatgaga aaacactggt gctctccacc tcgatcgata acgtgaaggc
    17161 cattgtcgac gcgtatcgcg atcaaagccg gtcgtccggc ggggagacgg ccttccaggc
    17221 catcgccgat cgattgggtg aggtcgccgc ggccgctttg agtcgcgccg aagtcgtatg
    17281 ccgcagcctg aacgaggcaa acatcagcgc acagaccgaa gccgtgaaag cgatactggg
    17341 tccggcgcgg gcgctgcacg cttaccaagc gttcggcatc ggctatcgtt acgagaacga
    17401 gaaaccgatc ggcctgctgg tttttcatta cgcgaatgcc gccgacgccc aggccgatct
    17461 ggatgcgcgc cgccggctcg ccgacgaagc caccagcctt tcaacgcgac aaccgtacag
    17521 cgtagccctg ttcacgttga accaggcgtt ggttgacggc agcgacctca cgctacaggt
    17581 gagtccggtc aacgacctgc cgcgccgcct gttcgatatg gtccgcaggc gggatgtgat
    17641 cttcgctgtg tgcccccagt agtctgaaag gggtggccgg tagtaacgac tggagacaga
    17701 gccggaaagc ggctactacc cgccgccgcc gaacctggtc gagccgaagc gcggcttcgg
    17761 caaggtgtgg cgcgatcaac tcggcgggcc ggatgcggcg gtcaggtggg cggtggccga
    17821 agaaatcggc ggggagggag agtggcaaag cctgcagaac ggcgacgcga tcagtctcac
    17881 tcggtacggt gtgctgatcg tgctggccga tggaacgtgg aaatgagcct gcgcgccatc
    17941 atccgctgga tgagcgtggc agggctggtg atcggcgcgg cctgtgcgcc gccgcctgcc
    18001 ttgacgcaac ccacggcgac cgtcactccc acgcggccgc cgacgtggac gccaacgccg
    18061 ggccagagcg atactgagac accgacggcc acaccgcacc cgacacgcac gctcgtgccg
    18121 ccggccacgc ccgccgagca caaggtcgtc gtcgcgcagg ataacgcgtt ttccatccag
    18181 gtcccctcca actgggaggt caccgagggg acgcggcgtg tggaagggcg caccaactac
    18241 gaagtcaagt atttcgccgc agtcgggcca ggcgatccgc cacaacccgg cgtgatcatc
    18301 ttttacgaat ggccgtcgtc gctcagcatc accaatgaca acgcatggga agtcaccaat
    18361 gacaacgcat gggagtctgc gtacgcgctg acggcgctcg tgatcaagtc ctgcgccacg
    18421 cagcttggcc aggagaagct gccggtcgat ttcggcggcg agccggcctt cggcgccgag
    18481 tacgtcgacg cgtgcggcgc ggcagggttg gtggccggcg ccgtgcacaa cggcgtcaat
    18541 tacggcgcgc tgttcgaagc gcccgcgccc tacttcggcg agtggttcga tacgctgcgt
    18601 gaaatcttgc tgagcatcac gttcggaagg tgatggcgta tcgcatagcg ccgtttccag
    18661 ccgcaaccaa acatgtagga ggttgcccct tcgacttgca tctctgaaaa atttggctat
    18721 aatctagcca gatatgcgct acgccatcat cctctcgcct gaagccgtcg aggatttgcg
    18781 aggcctgaaa gccgatgtgc gcgctacggt gcgtgacgcc attgaaatcc atttgcggca
    18841 cacaccgact aagaccagta a
//
LOCUS       DBNHXB010000057         9362 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2024726, whole genome shotgun sequence.
ACCESSION   DBNHXB010000057 DBNHXB010000000
VERSION     DBNHXB010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9362)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2024726"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="AC162G_02130"
     CDS             <1..806
                     /locus_tag="AC162G_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR5074643.1"
                     /translation="AMPTADLQREVEARRAQAARLEQERLDLQIEIEAFRLAYLSHAG
                     AAQAELQALEIHIAEYQLRNELMRLRGKSLSASQLEAEVDWQMRRRREEFTAYQDSVF
                     RAERETSQPPPTPDPATQNDLKSLYRDLAKRAHPDLATDDAERASRGALMVEINEAYR
                     RSDLTALKAIFARWSVSGGEVPDDRERLREEIARLDGLISKIRAEIADLNRSDWMTMK
                     LEAALARARGIDWFQLARRQTEMRAAERRAELDDLIVEFMDRVRSAGLA"
     gene            939..3017
                     /locus_tag="AC162G_02135"
     CDS             939..3017
                     /locus_tag="AC162G_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5074644.1"
                     /translation="MSSTLWQPLGELAMRMHAAAHEEDVFRIAGDGLKALGLRSAIAL
                     LDSALERFRLVYASTARPVLIAAERLFGARGIGYEIAIGESFTLGRAVHDRAMIYVRD
                     ALSYEGVPDIRPRTIKRGIELLDAAPHIAAPLIARDRVLGAIAVQSSRLAEEDGPAVM
                     AFAGHLALAVDALRRQAEAASRERQLAIIVQINQNVSADLTNVARAYDSVLHEVKRLV
                     SFDEAEVALIEPESRQVRISSPGTTLPGEDSGASTYTLRGSVIEWMVTYGQPYVAKDT
                     NVDREFAEARTASARRMRSLIALPMRHRGQVLGAFILLNRTPYFYAESDCDRLAPIVD
                     QMASTLVNYRLFEQVERGRRQLQAVLDSTGDAVIAVDMSGRITLMNPAAVRLFGFEDS
                     PEAGRPVWDVVNVPVLAGAFRQALAGKFSAPLGLEVPLLSDRVLFADLAPIQDSRAHA
                     LGWMAVIRDITHHKQMEALRSETIATAAHDMKSPLHLVAGALGVLAEDAPLLAEEQRE
                     AIAIAQSGLRRMRRLIDEILDLKKIEDGFGIDKRECDLASVLRSVAGEAAAPAAARSQ
                     ELVLDMPGELPVIRADPDRLHQVFANLVSNAIKYTPSGGAIRIRALASGDLVHVTVID
                     NGSGISVEDQARIFEKFYRTRSSSKIDGTGLGLAIVKSIVEQHGGHVIVQSTPGQGSE
                     FVVSLPVTSD"
     gene            3158..5743
                     /locus_tag="AC162G_02140"
     CDS             3158..5743
                     /locus_tag="AC162G_02140"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HIR5074645.1"
                     /translation="MAPKEIGVVRQIDIDSEMQQAYLSYAMSVIVARALPDARDGLKP
                     VQRRILYAMHDMGLRPDAPYKKSARIVGEVLGKYHPHSDAAVYDTMARLAQDFSMRYL
                     LVDGPGNFGSVDGDAPAAMRYTEARLGPLATEMLRDLEKQTVDFTYNFDGTLTEPSVL
                     PSALPNLLVNGSTGIAVGMATNVPPHNLGEVCDALCYLIERWDRLDRVPVDELMQFIK
                     GPDFPTGGVLFRYSDAVEGGDAIRAAYATGRGRVIVQAKAYIEEAERGKSRIIVTELP
                     YAVNKAALIERIADLVRDGRIEGITDLRDESDRNGMRIIIELSRSIEDPRKTLGLLYK
                     YTPMQSTFGMNLLALVDGEPRLLPLKRALQIYVEHRQTVVMRRSEHDLARAKERAHIL
                     EGLLKAIANLDEVIRIIRRSADAEAAKQSLMKRFRLTEMQATAILDMPLRRLARLERE
                     KLEAEYKEKKKLIKYLEDLLGSPKKILGVIKDELTALKVHYGDARRTVVVERKVGDQI
                     VTAAELLPDESTWVTINRDNKMLRMPVGPTIVKGRAPVAITHSNTRDVYVAINARGQC
                     GSIAVHQISNESMPVAEVVGLPSDQRLVAAFSIPRGILAEQDGAENEHKAYLFLATRS
                     GSVKRVAASDVIRQTGAFSVMNVPEDDEIVSAFATEGGGEVVVVTAAGKAIRFSEEEV
                     RPMGLAAGGVAGIRLQEKDSVVAAALVRPRSDLLIVTEAGRGKRSSLAEFPSQGRAGQ
                     GVVAANLGGKDRIVGAAVVQAPDPLAVVTSRGKARLIRARALKSVQRASPPVAAIHLT
                     KADSVAHIVVPIGRIAPPEPPAPAKPKRAARRASAMAKGKPAKTRPDKAKSKKSASRK
                     AARKS"
     gene            6187..6309
                     /locus_tag="AC162G_02145"
                     /pseudo
     CDS             6187..6309
                     /locus_tag="AC162G_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012367.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
     gene            6334..7125
                     /locus_tag="AC162G_02150"
     CDS             6334..7125
                     /locus_tag="AC162G_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5074646.1"
                     /translation="MRRSNLSPATIERLQARIRDGEKARDELVLHNMRLVISVARQYQ
                     NRGLPLSDLIQEGNVGLVKAADKFDVERGVRFATHAVWWIRQSVTRALSNKSRTIRLP
                     IQFAQRASELFRTRERLEQDLGRPPTPDELSVAMRWPVEKVRHMLTVSQPPLDLDAKH
                     GAENSEDELLDFVADESGQQPEGVASDNMLLEEITTLLEELPANEARVLRMRFGLSDG
                     RALSLSEIGRRMGLSRERVRQINNSALSTLRASAARHDLRDYLFN"
     gene            complement(7202..8275)
                     /locus_tag="AC162G_02155"
     CDS             complement(7202..8275)
                     /locus_tag="AC162G_02155"
                     /inference="COORDINATES: protein motif:HMM:NF021539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HIR5074647.1"
                     /translation="MSDGIQASSEPVWSYRGYHLKPSEFTTAMVHFYRGEVQRANVWR
                     QRLDNTTNWAVITTGAAISFALGTESGHHGVILLNTLLITLFLYLEARRYRYYELFAS
                     RVRLMETDFFATMLVPPFAPSADWAEGLAENLLHPQFTISMWEALGRRFRQNYLWIYV
                     VLAIAWILKSVLFPIPVTSWAEFIQRAALGTIPGEVMLLIGFIFNGLLFLIGIATLGL
                     KHATGEVLPRYTDTSLVHSLSSLFEHDKDQEASDRRPWFRHARKRPQAVALIISQHND
                     EVGKAILAEMKRGATRLEGVGVYTGQSQPVLMTALTLTEINHLKAIVSQADPNAFVIV
                     MPAQEVLGRGFQPLPRKQAATQP"
     gene            8477..>9362
                     /locus_tag="AC162G_02160"
     CDS             8477..>9362
                     /locus_tag="AC162G_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5074648.1"
                     /translation="MKVDTILAHGAVLTMDDAFTIVPDGALAIRGEDIVAVGASPEVL
                     AAYSADEIVDCSSCAIIPGLINAHTHVPMTLLRGLVDDLRLDVWLMGYMMPVEREFVN
                     PEFVRVGALLGCAEMIRSGVTTFCDMYYFEDDVAQATAEAGLRGVLGQTVLIFPSPDA
                     QSYEDSIEAGRKFIEKWKGHPLVVPAIAPHAHYTCPPEVLRACVDLATEFSVPLHTHV
                     SETALEVEESRKQHGMPVVPWIKRNGLLDTKLIAAHCVHVDPGEIRTLQRAGAGVAHN
                     PSSNLKLASCVAPVAQMLE"
BASE COUNT         1798 a         2812 c         3046 g         1706 t
ORIGIN      
        1 gagcaatgcc cactgccgat ttgcagcgcg aagtcgaggc ccggcgcgca caagcggccc
       61 ggctggagca ggagcgcctc gacttgcaga ttgagatcga ggcgttccgg ctggcatatt
      121 tgagccacgc cggcgcggcg caggccgaac tgcaggcgct ggagattcac atcgccgaat
      181 accagctccg aaacgaactg atgcgcctgc gaggaaagtc cctctcggca tcccagttgg
      241 aggcggaagt cgactggcaa atgcgccggc gacgcgagga atttactgcg tatcaggatt
      301 cggtctttcg agcggagcga gagacatcgc agccgccgcc cactccggat ccggccacgc
      361 agaacgatct gaaatcgctt taccgtgacc tggcaaagcg cgcccatcca gacctggcga
      421 cggatgacgc agaacgagca tcccgcggcg ctttgatggt cgagatcaac gaggcttatc
      481 gacggtcaga tctgacggcg ctgaaggcta tctttgcgcg ctggagtgtt tcgggcggtg
      541 aagtccccga cgacagggag cgcctgcgcg aggagatcgc gcggctcgat ggtctgatct
      601 ccaagatacg agcggagatc gccgatctga accggtcgga ttggatgacg atgaagctgg
      661 aggcagccct ggcgcgcgct cgcgggattg attggttcca gctggcgcgc cgccagactg
      721 aaatgcgcgc cgccgagcgc cgggccgagt tggatgattt gattgtcgag ttcatggatc
      781 gggttcgcag cgcgggcctg gcataaatcc ttgccccggc gatgatgtgt tatactgccc
      841 gcggttgaga acctcaagcc gtggacctgg ccagtctatc ggataagatg ctagacctca
      901 caggtctccg agacctgtga ggtctgcctg gagaaatggt gtcgtctaca ctgtggcagc
      961 cattgggcga gctggccatg cgcatgcatg cggctgcgca tgaagaagac gtcttccgca
     1021 tcgccggaga cgggctgaag gcgctcggcc tccgctcggc gattgcgctg ctggacagcg
     1081 cgctcgagcg ttttcgcttg gtctatgcgt cgacggcgcg cccggtgctc atcgccgcag
     1141 agcgcttgtt cggcgcgcgc ggcatcggtt acgagattgc tatcggcgag tcgttcacgc
     1201 tgggccgggc cgtgcacgac cgcgccatga tctacgtgcg cgacgcgcta tcgtatgagg
     1261 gcgtgccgga cattcggcct cggacgatca agcgcggcat tgaattgctc gacgcggcgc
     1321 cgcacatcgc cgcgcccctg atcgcccgtg accgggtact cggcgccatc gccgtgcaat
     1381 cgtccagact cgccgaggag gacgggccgg ccgtgatggc atttgcgggc catctcgcgc
     1441 tcgccgtcga cgcgttgcgc cgtcaagccg aagcggccag ccgcgagcgc caactggcca
     1501 tcattgtcca aatcaatcag aacgtcagcg ccgatctgac gaacgtcgcg cgcgcctacg
     1561 actccgtgct gcacgaggtt aagcgcctgg tctcgttcga tgaagccgag gttgcattaa
     1621 tcgaaccgga atccaggcag gtgcgcattt cctcgcccgg gacgacgctt cccggcgaag
     1681 acagcggcgc gtcgacgtac accttgcgcg gctcggtgat cgagtggatg gtgacgtacg
     1741 gccagccgta cgtcgccaag gataccaacg tcgatcgaga gttcgcggaa gcgcgcacgg
     1801 caagcgcgcg gcgcatgcgc tcgctgatcg cgttgccgat gcgacatcgt ggacaggtcc
     1861 tgggcgcttt cattttgctt aaccgcacgc cctatttcta tgccgaatcc gactgcgatc
     1921 ggcttgcccc catcgtcgat cagatggcga gcacgctggt caattatcgg ctgttcgagc
     1981 aggtcgagcg cggccgccgc cagttgcaag cggtgctcga ctcgacgggc gacgccgtga
     2041 tcgccgtcga catgtccggc cgcattacac tgatgaatcc ggcggcggtg cggctgtttg
     2101 gattcgagga ctctccggag gccgggcgac ccgtgtggga cgtcgtgaat gtgcctgtgc
     2161 tggcgggggc atttcgacag gcgctggccg gcaagttcag cgcgccgctc ggcttggagg
     2221 tgcctctgtt atccgatcgg gtcctgtttg ccgatctggc ccccattcaa gactcgcgcg
     2281 cccacgcctt gggttggatg gccgtcattc gggacatcac gcatcacaag cagatggagg
     2341 cgttgcgctc cgagacgatc gctacggccg cccacgacat gaaaagcccg ctccatctcg
     2401 tcgccggcgc gctgggcgtc ctcgccgagg acgcgccgct gttggcggaa gagcagcgcg
     2461 aggcgatcgc gattgcgcaa tcgggcttgc ggcgcatgcg ccgcctgatc gacgagattc
     2521 tcgatctcaa gaagatcgag gacgggttcg gtattgacaa gcgcgagtgc gatctcgcca
     2581 gcgtgctgcg ctccgtggct ggcgaggccg cggcgccggc tgcggcgcgc tcgcaagagc
     2641 ttgttctcga catgcccggg gaattgcccg tgattcgggc cgatccggat cgcctgcacc
     2701 aggtcttcgc aaatctggtg agcaacgcaa tcaagtacac cccatccggg ggggcgatca
     2761 ggattcgcgc gctcgcgagt ggcgacctcg tccacgtcac ggtgatcgac aatggctcgg
     2821 gcatcagcgt ggaggatcag gcgcgcatct tcgaaaagtt ttacaggacg cgatcctcgt
     2881 cgaaaatcga tggcacgggg ttaggcctgg ctatcgtcaa gtcgatcgtc gagcagcacg
     2941 gcggccacgt gatcgttcaa agcacccccg ggcagggcag cgagttcgtc gtgtccttgc
     3001 ctgtcacgag cgactagtgt tattctgagc cttgccctgc ctgcacctgt ggcgcaggca
     3061 caggtgagcg gagtcggggg tagcgaagaa tctcggcgct cgcggcggcg gatgatcaat
     3121 ccgccgcttt gtgaacttct cattctgaag ggcggttatg gctccgaaag agatcggtgt
     3181 tgtccggcaa attgacattg acagcgaaat gcagcaagcg tacctgtcgt acgcgatgag
     3241 tgtgatcgtc gcccgcgcgc tgccggatgc gcgcgacggg ctgaagccgg tacagcggcg
     3301 catcctgtac gccatgcacg atatgggcct gcggcctgac gcaccgtaca agaaatcggc
     3361 gcgcatcgtc ggagaagtgc tgggcaagta ccacccgcac agcgacgcgg cggtgtacga
     3421 cacgatggcg cgtctggcgc aggacttctc gatgcgctac ctgctcgtcg atgggccggg
     3481 caacttcggc tcggtagacg gcgacgcgcc cgcggcgatg cgctacaccg aggcgcgcct
     3541 cgggcctttg gcaaccgaaa tgctgcgtga cctcgagaaa cagaccgtcg atttcacgta
     3601 caacttcgat gggacactga ccgagccgag tgtgctgccc tcggcgctgc ccaatctgct
     3661 ggtcaatggc tcgaccggaa tcgccgtcgg catggcgacc aacgtccctc cgcacaacct
     3721 gggcgaggtg tgcgatgctt tgtgctacct gattgagcga tgggacaggc tggaccgcgt
     3781 tccggtcgac gagctgatgc agttcatcaa gggcccggat tttccgacag gcggcgtgct
     3841 gtttcggtac agcgatgcgg tcgagggcgg cgacgcgatc cgggccgcct atgccaccgg
     3901 ccgcggccgg gttatcgttc aggccaaagc ctatatcgag gaagccgagc gcggcaagag
     3961 ccgcatcatc gtcaccgagc tgccgtacgc cgtcaacaag gctgccttga tcgagcgcat
     4021 cgcggatctg gtgcgcgacg gccggatcga aggcatcacc gacctgcgcg acgagtccga
     4081 ccgcaacggc atgcgcatca tcatcgagct gagccgaagc atcgaggatc cgcgcaagac
     4141 gctgggcctg ctgtacaagt acacgccgat gcagtcgacc ttcggcatga atttgctggc
     4201 gctggtcgac ggcgagccgc gcctgctgcc tctgaaacgc gccctgcaaa tctacgtcga
     4261 gcaccgccag acggtcgtga tgcgccgctc tgaacacgat ctggcgcgcg cgaaagagcg
     4321 cgcgcacatc ctggaaggtc tgctcaaggc gattgccaat ctcgacgaag tgatccgcat
     4381 cattcgccgc agcgcggacg ccgaggcggc caagcagtcg ttgatgaagc gcttccggtt
     4441 gaccgagatg caggccacgg cgattctcga tatgccgctg cgccggttgg cccgcctgga
     4501 gcgcgagaag ctcgaagccg aatataaaga gaaaaagaag ctcatcaagt atctcgaaga
     4561 tctgctcggc agtcccaaga agatactcgg cgtgatcaaa gacgagctga ccgcgctgaa
     4621 ggtccattac ggcgacgcgc ggcgcacagt cgtcgtcgag cgcaaagtgg gcgaccagat
     4681 cgtcaccgcc gccgagctcc tccctgacga gtcgacgtgg gtgacgatca accgcgataa
     4741 caaaatgctg cgcatgccgg tcggcccgac gatcgtcaag ggccgggcgc cggttgccat
     4801 cacgcattcc aacacgcgcg acgtgtacgt ggcgatcaat gcgcgcggcc agtgcggatc
     4861 gatcgccgtg caccagatca gtaacgaaag catgcccgtc gccgaggtcg tcggactgcc
     4921 cagcgaccag cgcctggtgg cggctttctc cattccgcgc gggattctcg cggaacagga
     4981 cggcgccgag aacgagcata aagcttacct gttcctggcc acgcgcagtg ggtcggtcaa
     5041 gcgcgtcgcc gccagcgacg tgattcgcca gacgggcgcc ttctccgtca tgaatgtgcc
     5101 ggaagacgac gagattgtct cggcctttgc gacggagggg ggcggcgagg tggtcgtggt
     5161 gacggcggcc ggaaaggcga ttcgcttctc agaggaagag gttcggccga tggggcttgc
     5221 cgcgggcggc gtcgccggca tccgactgca ggagaaggac tcggtcgtcg cagccgctct
     5281 ggtgcggccg cgcagcgatc tgctcatcgt cacggaggct ggccggggca aacgctcgtc
     5341 gctggccgag tttccaagtc agggccgggc gggccagggc gtcgtcgccg ccaacctcgg
     5401 cggcaaggat cggattgtcg gcgcggcggt cgtacaagcg cccgatccgc ttgccgtggt
     5461 gacgtctcgc ggcaaggcaa ggttgattcg cgcgcgcgcg ctcaaaagcg tgcagcgcgc
     5521 cagcccgcct gtcgccgcca tccatctcac caaagccgac agtgtggcgc acatcgtcgt
     5581 tcccatcggg cggattgcac cgccggagcc gcccgcaccg gccaaaccga agcgtgcagc
     5641 gaggcgggct tcggcgatgg ccaaaggcaa gcccgcaaag acgcggccgg acaaggcaaa
     5701 atcaaagaaa tcggcttcta gaaaggctgc ccgcaagtcg tagcagcttg gtgaatacct
     5761 actcccgtct ccagacgatg accagtcgag tgaaccgaag cttcggcttg agcgtatctt
     5821 atttcatcac acccagcagg cttccggagg gcctaacaga gacggctcct tgggacgcac
     5881 cccgcagcgg agtgggcacg cttgtcctgc gcagtccccg accgaaggga gcgggaagcg
     5941 acgccgaagg gccgctgatt cagcgcgttt caaggcaaat cgccgggcga tagaacgcct
     6001 gtttcttgtc gttcgggtgg gaccacaagc ctcacaatct aatcaaatct taatgatttc
     6061 cttgtaaaat atcgagtaaa atgcatataa ccacagacca ttgacatttc tgagaatttg
     6121 tatcatactt actataaaaa tctgattacc ggtgtgacga gacttagtgt atagggagag
     6181 tgtaagatgg ctacgagtga ggagagccac agaggcgccc ctgacgtatt ggaacagtac
     6241 ttgaaggagg tccgaccgat ccccttgttg actgctgagg aggaaagaca cctgggcgat
     6301 cgtcttcggg caagccgcga ggccagacgc aggatgaggc gcagcaacct gtcgccggca
     6361 acgatcgaga ggctgcaagc gagaattcga gacggggaga aggcgcgcga cgagctggtg
     6421 ctgcataaca tgcgcctcgt catcagcgtg gcgcgccagt atcagaaccg cgggctgccg
     6481 ctgagcgact tgattcaaga gggcaacgtc gggctggtga aagccgccga taagttcgac
     6541 gtggagcggg gtgtacgctt tgcgacgcat gccgtctggt ggattcgcca gtcggtgacg
     6601 cgtgccttga gcaacaagtc gcgcaccatt cggctgccca tccagttcgc gcagcgcgcc
     6661 agcgagctgt tccgtactcg cgagcgcctg gagcaggacc tggggcgacc gccaacacct
     6721 gacgaattgt cggttgccat gcgctggccg gtggaaaaag tccggcacat gctgacggtg
     6781 agccagccgc ctctcgatct cgacgccaag cacggcgcgg agaactccga agacgagctg
     6841 ttggacttcg tcgccgacga gtcaggtcag cagcccgagg gcgtggcctc agacaatatg
     6901 cttctggaag agatcacgac gctgctggag gagctgcctg ccaacgaggc gcgggtgctg
     6961 cgcatgcgtt tcggcttgag cgatgggcgt gcgctcagtc tgagcgagat cgggcggcgc
     7021 atgggtctga gccgcgagcg ggttcggcag atcaacaatt ccgcgctgag cacgctgcgc
     7081 gcatctgccg cccgtcacga ccttcgagac tatctgttca actgagccga gttgagagcg
     7141 aatcgggccg gtgcacagcg caccggcccg attcgctttt aggtctggag ttggcaaatg
     7201 attatggctg cgtggcggct tgtttccgcg ggagcggctg aaaacctcgg ccgagcacct
     7261 cctgggcagg catcacgata acgaacgcat tcggatcggc ctgcgataca atcgccttca
     7321 agtgattgat ctcggttagc gtgagcgccg tcatcagcac gggttgtgac tgtccggtgt
     7381 acaccccgac tccttccaat cgagttgcgc cgcgcttcat ctccgccagg atggccttgc
     7441 cgacttcatc gttatgttga ctgatgatca gtgcgaccgc ctgcggccgc ttgcgtgcgt
     7501 ggcggaacca gggccgccga tcgcttgctt cctggtcttt gtcgtgctcg aacaatgacg
     7561 acaaggagtg gaccaggctg gtatcggtgt agcgcggcag tacctcgcca gtcgcatgtt
     7621 tcaatccgag ggtcgcgatg ccgatcaaga atagcaggcc attgaagata aacccaatga
     7681 gcagcatcac ctcgcctggt atcgtgccca acgctgcccg ttgaatgaac tcagcccacg
     7741 aggtaaccgg gattgggaat aaaacgcttt tcaggatcca ggcgatggcc agaacgacgt
     7801 atatccacaa gtagttttgc cggaagcgcc ggcccaacgc ctcccacatg gagatggtga
     7861 attgaggatg gagcaggttt tcagccaggc cctcggccca gtcagcggac ggcgcgaagg
     7921 gcggcaccag catcgtggcg aagaaatccg tctccatcaa gcgcactcgc gacgcaaaca
     7981 actcgtaata acgataacga cgcgcttcca gataaagaaa cagcgtgatg agcagcgtgt
     8041 tcagcagaat cacgccgtga tggccggatt ccgtgccgag agcaaagctg attgctgcgc
     8101 cggtggtgat gacggcccaa ttggtcgtgt tgtccagacg ctgccgccac acgttcgcgc
     8161 gctgcacttc tcctcggtag aagtgaacca tcgccgtggt gaattcgctc ggcttcaggt
     8221 gatagccccg gtacgaccat accggctcgg aggatgcctg tattccgtca ctcatgatct
     8281 cgcgctcccg agtatgttgg atcgttcgcc gaccgcagta taacaccgaa cgccgatgcc
     8341 ggtttgcgcc cgagtgcgaa atgactataa tcgtctgacc acggattaca cggatgtcac
     8401 agatggaaac gattcaacgt ccgtgtcatc cgtgccatcc gtggttagtc ttttgcttgc
     8461 agccaggagt cgcgctatga aagtcgatac gatactggcg cacggcgccg tgctgacgat
     8521 ggacgatgcg tttacgatcg ttcccgacgg cgcgctggca atccggggcg aagacatcgt
     8581 cgccgtcggc gcatcgccgg aagttcttgc cgcgtattcg gcggatgaga tcgtcgactg
     8641 ctcaagctgc gccatcatcc ccggcttgat caatgcgcac acacacgtcc caatgacgct
     8701 cttgcggggc ctggtcgacg atttgcggct ggacgtgtgg ctgatgggtt acatgatgcc
     8761 ggtcgagcga gagttcgtga atccggagtt tgtgcgggtc ggcgccttgc tcggctgcgc
     8821 cgagatgatc cgttccggtg tgacgacatt ctgcgacatg tactatttcg aggacgacgt
     8881 tgcgcaggcg acggccgaag cgggcttgcg cggcgtcctc ggccagacgg tgttgatctt
     8941 tccgtcgccg gacgcgcagt cctacgaaga ttcgatcgag gccggacgca agttcatcga
     9001 gaagtggaag gggcacccgc tcgtggtgcc tgccatcgcg cctcacgcac attatacctg
     9061 cccgcccgag gtgctgcgcg cctgcgtcga tttggcgacc gagttcagtg tgcccctgca
     9121 cacacatgtc tcggagacgg cgctcgaggt ggaggagtcg cgcaagcagc acggcatgcc
     9181 ggtcgtgccc tggatcaaga ggaacggcct gctggatacg aagctcattg cggcgcattg
     9241 cgtccacgtc gatccgggcg aaatacgaac gcttcagcgc gcgggcgcgg gcgtcgcgca
     9301 caatccctcc agcaacctca agctcgccag ctgcgtggcg cccgttgcgc aaatgttgga
     9361 gg
//
LOCUS       DBNHXB010000058         2414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2528005, whole genome shotgun sequence.
ACCESSION   DBNHXB010000058 DBNHXB010000000
VERSION     DBNHXB010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2528005"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2281)
                     /locus_tag="AC162G_02165"
     CDS             complement(<1..2281)
                     /locus_tag="AC162G_02165"
                     /inference="COORDINATES: protein motif:HMM:NF052741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5074649.1"
                     /translation="MAIHLIPTKITIPRRDSGVVRRPRLLEFLHENLERKLLLVTAPA
                     GYGKTTLVIDFASEVDLPVCWFTLDEGDRDPSSFLAHVVASIRQKFPNFGERSMSMSQ
                     SGMMVARAAAGALVADMVDDVPEYFVLVLDDWHLVGEEAVIRELLDHLLHYLPEHAHI
                     VVAGRTLPRGPLIRLTAQGAVAGLGPGDLRFTPSEVREVLAQKYDLEITEEQAVKLVD
                     ESEGWITGILLTSQAMWKSLLAGLAHAAGAPGALYEYLAGEVFDQLSLPLRRFLLESA
                     VPRQFTAELCDEVRGVPGAGVWIDQVEARSLFLTRVEVDGDTWFRYHHLFRDFLLARF
                     KRDDPQAVARLNLRSGEFFEARQEAEEAVEHYLAGGAPERAARVMDTHSRRLYIAGRT
                     QTLLNWTALLPPLYRPLAPELVLFQGQTLTERGKPKEALTVLKEAEAVFQARGDEIGQ
                     VRAILPQGWAYYALGELRESLAVGQRVRDRLDDAHADHVVWRAQALRLLGLCHSGMGQ
                     LQMGEAFLSDALALYRSSASSERRDFDLARTTHDLAFTLRSQGRLEEAAALEAESLAM
                     WRRIGNPGPLALSLNNVGYDRYLAGDYDGALATYTEAIAKAEDAGDRRTQALILEGFA
                     AVFRDRGEFQRAIEVYEDVFNMASEIGDQSLVSLVLDGLGHAHRLSDGLDRAVSLFEQ
                     ARSVAFRGGIDSQAILSEASISMGRFEQGDVSPGIQGLEHAGRALRESGSHLDLARVL
                     FWLARAYFETGRHAEARESL"
BASE COUNT          442 a          829 c          740 g          403 t
ORIGIN      
        1 cgaggctctc ccgcgcctcg gcgtgccggc cggtctcgaa ataggcgcgg gccaaccaaa
       61 acagcacgcg cgccaggtcg agatgcgagc ctgactcacg cagcgcacgc ccggcgtgct
      121 ccaacccttg tatgccgggc gagacgtctc cttgctcgaa acgccccatg ctgatcgacg
      181 cttccgaaag tatggcctgt gaatcgatcc ctcctcgaaa cgcgacactg cgtgcctgct
      241 cgaataacga cacggctcgg tccagaccat ccgacaggcg atgcgcgtgt ccgagaccat
      301 cgagcaccag gctgaccaga gactgatctc cgatctcact ggccatattg aatacatctt
      361 cgtagacctc aatcgctcgc tgaaactctc cacggtcgcg gaagaccgcg gcgaaacctt
      421 ccaggatcag cgcctgagtg cgccggtctc ctgcgtcttc ggctttggcg atggcttctg
      481 tgtatgtcgc cagcgcgcca tcgtagtcgc cggccaggta gcgatcatac ccgacattgt
      541 tgaggctcaa tgccagtgga cccggattgc cgatgcgtcg ccacatggcg agcgactcgg
      601 cctcgagcgc ggctgcctct tccaggcgcc cctgcgagcg cagcgtgaaa gccagatcgt
      661 gcgtcgtgcg cgccagatcg aaatcgcgcc gctcgctcga cgcgctcgaa cgatacaggg
      721 ccagcgcgtc cgacaggaag gcttccccca tctgcaactg gcccatgccg ctgtggcaca
      781 ggccaagcag ccgcagcgcc tgcgctcgcc acacgacgtg atcggcgtgc gcgtcgtcga
      841 gcctgtcccg cacacgttgc ccgaccgcca gggactcgcg cagttcgcct aaggcatagt
      901 acgcccagcc ttgtggcaag atcgcccgca cctggccgat ctcgtcgccc cgcgcctgga
      961 aaaccgcttc ggcctctttg agaacggtca gcgcctcctt cggtttgccc cgttcggtca
     1021 gcgtctgtcc ctgaaacaag acaagctccg gcgccagggg gcgatacagt ggcggcagca
     1081 gcgccgtcca gttgagcagg gtctgcgtgc gacccgcgat gtacaaccgg cgcgaatgcg
     1141 tgtccatgac gcgcgccgcg cgctccggcg cgccgcccgc caggtaatgc tcgaccgctt
     1201 cttcggcttc ctggcgcgct tcgaagaact cgccgctgcg caggttcagg cgtgccaccg
     1261 cctgcgggtc gtcgcgcttg aagcgcgcca gaagaaaatc gcgaaacagg tgatggtagc
     1321 gaaaccacgt gtcgccatcg acttccacgc gcgtcaagaa caggctgcgc gcctcgacct
     1381 gatcgatcca tacgccggcg ccgggcaccc cgcgcacctc gtcgcatagc tcggccgtga
     1441 attgccgtgg cacggcggac tcgagtaaga agcgccgcag cggaagcgac aactgatcga
     1501 acacctcgcc ggccagatac tcatatagcg cgcccggtgc gccagcggcg tgggccagcc
     1561 cggccagcag gcttttccac atcgcctgtg aggtcagcag gatgcccgtg atccatcctt
     1621 cggattcgtc gaccagcttg acggcctgct cctcggtgat ctccaggtcg tacttctgcg
     1681 ccagcacttc gcgcacctcg gaaggcgtga agcgcagatc gccggggccg agtccggcga
     1741 ccgctccctg ggccgtcagg cggatcagcg ggccgcgcgg cagggtgcgc ccggcgacga
     1801 cgatgtgggc gtgctcgggg aggtaatgga gcaagtgatc gagcaattcg cggatgacgg
     1861 cttcttcgcc gacgagatgc cagtcgtcca gcaccagcac gaaatactcc ggcacgtcgt
     1921 ctaccatatc ggcgacgagc gcgccggccg ccgcgcgggc gaccatcatg ccgctttgag
     1981 acatcgacat cgaccgctcg ccgaaattcg gaaacttctg gcggatcgac gcgacgacat
     2041 gcgccaggaa cgagcttggg tcgcggtcgc cctcgtccag cgtgaaccag cacaccggca
     2101 gatcgacttc gctggcaaag tcgatcacca gcgtcgtctt cccgtatccg gcgggcgccg
     2161 tcaccagcag gagcttgcgt tccaggttct cgtgcaggaa ctcgagcagg cgcgggcgcc
     2221 gcacgacgcc tgaatcgcgc cgggggatcg tgatcttcgt cggaataagg tgaatcgcca
     2281 tcgggaacac cggccagaac gcatgcgatt atacgctggt ataggtcacg cgccaactgg
     2341 acggccagcc tcgacggcct acacttgacc atcgctattg cggatgatac acttcatgca
     2401 gacttccgaa gtct
//
LOCUS       DBNHXB010000059        17896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3534774, whole genome shotgun sequence.
ACCESSION   DBNHXB010000059 DBNHXB010000000
VERSION     DBNHXB010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 17896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17896
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3534774"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..441)
                     /locus_tag="AC162G_02170"
     CDS             complement(190..441)
                     /locus_tag="AC162G_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR5074650.1"
                     /translation="MDQQTCQHLLGGLSEYLDGEASADLCAEVERHLAECADCRVLVD
                     TLRKTVSLYRELPQPEMPTAARERLYTSLDLAEFLAPKP"
     gene            complement(444..1049)
                     /locus_tag="AC162G_02175"
     CDS             complement(444..1049)
                     /locus_tag="AC162G_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5074651.1"
                     /translation="MSDHDADRRLLERIRGGDKSACAECIEQHSAEVYRLALRLTGNE
                     TEAEDIVQETFLSAFKAIDSFEGRSSLSTWLYRIATNAAMMRRRRVQPEAVSVDDPDL
                     FGDDLPTPRPLFDWCCLPERDFETAETRAELGRAIRDLPDKLRAVFVMRELEELSTEE
                     TAEALDLSIDVVKTRLHRARLWLRERLSLYFTELVRAREEG"
     gene            complement(1228..1638)
                     /locus_tag="AC162G_02180"
     CDS             complement(1228..1638)
                     /locus_tag="AC162G_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR5074652.1"
                     /translation="MTDRIQTSLGLAVRLAADFESAVARVTGALKAEGFGVLTEIDVK
                     DTLKKKLGVDFRPYKILGACNPPLAHRALTAAPEVGLLLPCNVVVAQLDDASCEISIV
                     DPLGMLGIVGRPELEPIAEEARIRLERVAAALKE"
     gene            complement(1749..2159)
                     /locus_tag="AC162G_02185"
     CDS             complement(1749..2159)
                     /locus_tag="AC162G_02185"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR5074653.1"
                     /translation="MDMIIDFPGGARVDAHFGPYTVRTDQPPQGGGDGSAPTPFATFL
                     ASIGTCAGIYVLGFCRQRGISAEGIRLIQRMQVDPFTGLVGKVLLDIHLPPDFPEKYK
                     AAVIRAAEQCAVKKHFEQPPAFEITTVTGEPAIA"
     gene            complement(2272..2493)
                     /locus_tag="AC162G_02190"
     CDS             complement(2272..2493)
                     /locus_tag="AC162G_02190"
                     /inference="COORDINATES: protein motif:HMM:NF022577.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP-like transmembrane domain"
                     /protein_id="HIR5074654.1"
                     /translation="MSFAKFMSSGLGRGLRIVAGIALIAIGLANNAGILAVIGLVPLL
                     AGLLDVCLIGALLFGAPLKGADVRARLNS"
     gene            2917..3225
                     /locus_tag="AC162G_02195"
     CDS             2917..3225
                     /locus_tag="AC162G_02195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR5074655.1"
                     /translation="MIARLWHGVTPASKADVYLDTLNQTGVGDLRATPGNRGVYVLRR
                     IDGDVAHFLLISLWDSMDAVKSFAGAEVDKARYYPEDREFLLEFEPTVTHYDVLVRDM
                     "
     gene            3232..4224
                     /locus_tag="AC162G_02200"
     CDS             3232..4224
                     /locus_tag="AC162G_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR5074656.1"
                     /translation="MILHHDVVYGPAEIGEPILVELMHSAAVQRLHGVLQHGISGLIG
                     ITRPTTRFEHSVGVMLLVRRLGASLEEQIAALLHDVSHTAFSHVIDYVFGGHDSQSYH
                     EEVKEEYLAGTDVPAILARHHFDWHDFLHEADFPLLEQPAPALCADRLDYFLRDTGNL
                     GLSTDDDVQRALKHLVVHDGRIAADDVDVARWMGYTYMAADRASWASFREVGLYELTA
                     RAVRTGLETGAIAEADIWSTDGQLWAKLHAASHPNLQSELSLISPDTQFVWDETAPTF
                     RVSTKLRTIDPDVVLDGRSQPLSALDAEFARHRDDYLRRNRGKWPMRVVPGAGG"
     gene            4354..5391
                     /gene="yedA"
                     /locus_tag="AC162G_02205"
     CDS             4354..5391
                     /gene="yedA"
                     /locus_tag="AC162G_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HIR5074657.1"
                     /translation="MTTHSLSGVPARWRMLVAFAAIYTIWGSTYLAIRFAIETMPPFL
                     MGGARYLIAGAILYALTRRSRAAAVPRIHWRSAFIVGGLLLLGGNGGVIWAEQVIPSG
                     LAALLIATVPLWMALLNWLRGDNVRPNLGVTLGLALGLLGIVFLVSPGESAGGDQVNS
                     LGVLVLVLAALSWSIGSLYSRKAQLPAEPLLATAMEMLAGGALLLVAGLLTGEAGQLR
                     LDQVSPRSLLALGYLIVFGSLVGFSAYVWLLRVSTPARVSTYAFVNPVVAVFLGWALA
                     GESLTPRTLVAAAVIVTAVVLIVANQARTLPQRNADVTGVEDRASPFRRAFALIASRV
                     PGRRVEAGGGN"
     gene            5603..6004
                     /locus_tag="AC162G_02210"
     CDS             5603..6004
                     /locus_tag="AC162G_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5074658.1"
                     /translation="MITILLVDDQPAVRQGLNMRLALEPDLEVIGEATDGVEALRRAH
                     ELHPDVVVMDIEMPRLDGIAATEALRTIEPGCCVVILTVHENSATRARAKDAGAAAFV
                     SKHAGEEALLAAIRGATGLANVSQIEIGPNP"
     gene            complement(5994..9653)
                     /locus_tag="AC162G_02215"
     CDS             complement(5994..9653)
                     /locus_tag="AC162G_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5074659.1"
                     /translation="MRNLQPSNLRLRLLTLVLLAIVPLLGLILYNAAERRDLATDLAH
                     TNALQIARLAASNQAQYIEQARQMLIRLAERPELRAAGPALSAVEGLPSCSAVFSSLQ
                     TQHQQNAPRYANLGLIDANGRVYCSVVTVSGPLDVSGQTYFQNAVQTLSFSLGDYEID
                     RAKGQTVLNVGYPLLDFNGRVRLVLFASLDLGWLNQLASQAQLPPGSTFIVTDRQGTI
                     LIRHPGDDYYIGQTLSTLVLAAIQAQPDGGAVEATDADGVPRLYGFSPLSSGTGDTRA
                     ATVSIGIPAREAFAEGNAILFRNLAALGLVALLAFGVAWIAGDRFILRPVQALVHATR
                     RLSAGDLGARSGLSRTDQQGEIGQLAHAFDQMAESLERREIERRQAEAALRESEERYR
                     TLARNFPNGAVILFDHNLRYTLADGAGLAETGLSKEMLEGRTIWEIFAPDTCEILEPH
                     YRAALAGTPNVFEIRFADRIYQVNALPIRNEPGDIVAGMVMTQDITERKQAFQLLEQR
                     VEERTHELSTLLKVARDVTSTLELEPLLGIILDQLKSVVDYAGASVLIVEGDRVRYGA
                     YRGPAPMDAVLHIQFPLQGSLIQQIVTDRQPVILPDVREEAAQTHSIGKLQPERIDGA
                     LSYIRSWLGVPLIVQEKAVGVLSVHHSQPGNFSSRHAELAVAFASHAAIAMENARLFE
                     AGQRRAEQFRVINEVGQRITSILAVDEILSQTVRLIRESFGYYHVHIGLIEKDVVAYK
                     ATAGVWWDEPDCRCCSSLQLRVGLDGITGLAAGRGEPVLVPDVSQEPRYIPVQDGQTG
                     SELVLPLKVKRRVIGVLNVESERLNAFDESDVTVLQSLANQLAVAIENARLFETEQRR
                     AEQFRAINEVGRHITSILTVDELLAQTARLIQETFRYYFTSIGLIEGDEVVVKYGDPL
                     RLRVGQEGIIGWVAGAGEPLLVPDVSQDPRYIASPSATRARSELAVPIKSRGQSIGVL
                     DVESDRSDAFDESDVTVLQSLANQLAVAIENARLYEQAQQLAALEERQKLARELHDSV
                     SQALYGIALGARTARTLLDRDPGKVGEPLDYVLSLSEAGLAEMRALIFELRPESLEME
                     GLVAALGKQTASLRARYNIEVRTAFCGEPQLALPAKEALYRIAQEALNNVVKHARASQ
                     VNVQLETSDGSVRLEIGDNGVGFDTHSAFPGHLGLRSMRERAEKVGGAFSVESAPGQG
                     TRIRVHFPLTAPPKD"
     gene            complement(9663..11018)
                     /locus_tag="AC162G_02220"
     CDS             complement(9663..11018)
                     /locus_tag="AC162G_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5074660.1"
                     /translation="MRKLLLSLSVLVASAAALAACQASSTPTPTVDRPVTLRFTYWGS
                     PIERVAVEQMVRAFHAENPDIQVEALYIPDSEYIARVSAMIAAGTPPDVGYLLDTHAP
                     QWASEGKVMDLTDIVRADSELSSRLPETFFPIGPGRTIGTSTATEIMLLFYNKDVFDA
                     AGVSYPPARAEDAWTWDEFLAAARRLTVDRDGRHPGESGFDPDHIQVYGVSFVKSAWY
                     SYPPFIYSNGGEIVNEDGTRLMIDSPEAVEAIQRLADLMWVEHVTPTPAQQERLPPSD
                     ALFQTGQLAMDIRGQWKLLDYASMEGLRYGVAVLPKMKEAKTLIFGSPTVIFSGTQHL
                     EAAIKFYKFHNDPRVVDLFAQGLWMPLQLSYYTDPQAIASWIDNPAHPPESRQAIVDY
                     ALCCVVRAPHYYVKNFGQISSEVIQPAINRVWNGEASAAQAMAEAVRAATPLMAGRWD
                     R"
     gene            complement(11023..11721)
                     /locus_tag="AC162G_02225"
     CDS             complement(11023..11721)
                     /locus_tag="AC162G_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5074661.1"
                     /translation="MPIRILLVDDHSVVRQGLRMFLALDPELEVIGEATDGAEAVRMA
                     RELKPDVVLMDMLMPVMDGVAATAAIRRELPDTEVIALTSVLEDEKVIGAVRAGAIGY
                     LLKDTEADELRRAIKAAANGQVQLSPKAAARLMREVRAPDSPEALTERETDVLRLLAQ
                     GKSNKEIAHQLSLSEKTVKTHVSNILAKLGVPSRTQAALYAVRIGLASIESAGPAPSQ
                     GSGQALSGGEGRSA"
     gene            complement(11721..13598)
                     /locus_tag="AC162G_02230"
     CDS             complement(11721..13598)
                     /locus_tag="AC162G_02230"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR5074662.1"
                     /translation="MPIRILLADDHSVVRQGLRLFLGLDSELEVVGEAANGAEAVHLA
                     EELKPDVVLMDLMMPVMSGLEATAAIRDKGLPTRVIVLTSVLENTAVTAVIRAGAIGY
                     LLKDTEADDLRRAIKAATAGRAQLSPQIAARLLAETRAQDTFETLTDRESEVLRLAAQ
                     GLSDQEIAAKLVVSEPVVHSYIGDILDKLRLANQAQAALLDFSNQLLDRPDPAALTDY
                     LVQEIPRLLHVDACALLLPEREPGYLNFRAASGWHRDPVAECVRLPFDEGNGLGWVMR
                     SQRPWQAEDLTRTSASWAPDCWQAEGFRGHALIPLLAEGRAIGVLVLNSREPRLLDDD
                     EVRFLRLLANQAALIIEQTRLHQVELQRQGLEGDLAAARQIQLGLLPESCPRVPGWEF
                     AAAYQAARLIGGDLYDFIEFPDALAPTGGRRIGVLIADVSGKSTPAALFMAHSRAIIR
                     ATALGQPSPALTFSQANDLIQKDNQTSHFVSAFYAILNTGSGHLTYANAGHPRPLHWQ
                     AASGQFQELAGRGMVLGVSEGVEYEERQIDLAPDDLLILYTDGISEAVNIDLELFGQA
                     RLLETVAAASSGVPQDVIEAILAAVQRFSGEAPQADDFTLVVVKRLGHVESPIPERQV
                     P"
     gene            complement(13719..15851)
                     /locus_tag="AC162G_02235"
     CDS             complement(13719..15851)
                     /locus_tag="AC162G_02235"
                     /inference="COORDINATES: protein motif:HMM:NF025670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HIR5074663.1"
                     /translation="MTTHFPLVSISLRLAALTLLLLLTGSWSLGSQEAAQQPAAGQGL
                     ASLPLDAQFAISRVLGRDDKDYHVIPTQAGFRAAHPTRALEADFTREGAQVRVGEVRW
                     RLALLGYGYGEALEPVAPVQPRAQENRVVYPRGEATEWYVNGPLGLQQGFTLLAPPAG
                     AERAGSSPLTLALGLPAGLQARVDADGATLTLAQADGQAVLRYKGLLAHDATGRRLPG
                     WLEVREARVLVHVEDAGAAYPLAIDLWVQRAKLTASDGAAVDGFGWSMAISGDTVVVG
                     AAVDDIDANADQGSAYVFVKPGGGWADMTQTAKLTTSDGAELDWFGYSAAISGDTVVV
                     GAPGGPTGSPSTVPGSAYVFVKPGGGWADMTQTAKLTASDGMANDRFGISAAISGDTV
                     VVGAVFDDIDANVNQGSAYVFVKPGEGWADMTQIAKLTASDGAALDRFGISVAISGDT
                     VAVGALLDDIDANADQGSAYVFVKPGGGWADMTQTAQLTASDGAADDWFGASVAISGD
                     TLVVGAYGDDVDTNADQGSAYVFVRPGGAWAEMTQTAKLTASGGAALDWFGWSVAING
                     DMVVVGAPFDNIGTNTYQGSAYVFVKPGGGWMDMTQSAKLAAFDGAADGSFGSGVAIN
                     GGTVGVGAYRDDVGANADQGSAYVFGLETPRPTDTPSPTPTDTPSPTPTDTPTPTSTP
                     SPAATDTPSPTPTSTPAPVLIYLPLVMK"
     gene            15933..16910
                     /locus_tag="AC162G_02240"
     CDS             15933..16910
                     /locus_tag="AC162G_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074664.1"
                     /translation="MIASRVLLIIHNPVVDPQQGAKLSQALDWNDPDALADQYIRDVR
                     QASHGIVDYQIVERVEVDDFPAKIDGFRYDAASYLRCIREQRGFHEPDAIDYLALIRE
                     FRMIEKVAANQIDEVWLIGFPYSGYYESTMCGRDAFWCNSPPVKGTEHSERRFVIMGF
                     SYERGMGEMLEDLGHRAEAIMTKVYEGVTGERNLWERFSRYDSIAPGRASAGNVHFAP
                     NSERDYDWGNPRPVSSDCDDWLNFPSLTGARRMVDCRDWGNGDIRLHHLWWLERLPHA
                     EGETDGVRNNWWTYVVDPNTVQNSGCLTLFLSWFQRLSTPRSHRTAYHA"
     gene            16918..17688
                     /locus_tag="AC162G_02245"
     CDS             16918..17688
                     /locus_tag="AC162G_02245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5074665.1"
                     /translation="MSSLSFDRAAPIYDSTHELPAEISERATQTILGLAPSGLPILEA
                     GVGTGRIAVPLMRSGAPLVGVDLSLDMMQRLRAKHGAASLAQADASRLPFPDATIGAV
                     LTVHVLHLVGPWREALREFRRVLWPGGVYLNSHNYRHADSPNRRLRDRWHALVETRGW
                     TWRRPGIQDREELIEELRAMGAQVEEVTIAEWTGSVTPRQELSDIAARTHSDTWQIPD
                     DALAETVAELTAWAATQYDDLDAPIAVKRRVGFDVIHF"
BASE COUNT         3217 a         5750 c         5670 g         3259 t
ORIGIN      
        1 tcccgccgtc gcctctgtga caaagaacgt gcctaaaagc ggctaagggt ctgtgcccaa
       61 agcacagagc cttagccgtt tttggcctta cggcaaggga cgtatctgct cctgggacga
      121 atccgagact tgtgtcgtct atcccgttag atgcgaccgg agcagaatgg tacgagtcgt
      181 ccaggggcgc tacggcttcg gggcgaggaa ctcggcaaga tcgagcgatg tgtacagccg
      241 ctcgcgtgcg gcggtgggca tctcgggctg gggcaattcc cggtagagcg ataccgtttt
      301 gcgcagcgta tccaccagca cgcggcaatc ggcgcactca gctagatgtc gctcgacctc
      361 ggcgcacaaa tcggccgagg cttcgccgtc gaggtactcg gacaatccgc ccagcagatg
      421 ttggcaggtc tgttgatcca tagtcaccct tcttcgcggg cgcgcaccag ttcggtgaag
      481 tacagcgaca ggcgctcacg cagccacagc cgcgcgcgat gcaggcgggt cttgacgacg
      541 tcgatcgaca gatcgagcgc ctcggcggtc tcttcggtcg atagctcttc gagttcgcgc
      601 atgacgaaca cggctctcag tttatccggc aggtcgcgga tcgcccgccc cagctcggca
      661 cgagtttcgg cagtttcaaa gtctcgttcc ggcaggcagc accagtcgaa caacggccgc
      721 ggcgtcggta gatcgtcgcc gaacaggtcg gggtcgtcga ctgaaacggc ttcgggttga
      781 acgcgccgtc gtcgcatcat ggccgcgtta gtggcgatgc gatacagcca tgtgctcaag
      841 ctcgatcgac cctcgaacga gtcgatggcc ttgaacgcgc tgaggaacgt ctcctgcacg
      901 atgtcctctg cctccgtctc gttgccggtc aggcgcagcg ccaggcggta gacctcggcc
      961 gagtgttgtt cgatacactc ggcgcaggcg gatttatcgc cgccgcggat gcgttcgagc
     1021 aggcggcggt cggcgtcgtg atcgctcact agatttagat gcagctccag gcaaaaggat
     1081 acacgccagc ggggattata cagcaaacag tccgcggccg aatcggcgcg ggctgccgaa
     1141 gcgttcgggg atgaagatcg ttgtacagaa cggcggccgg ctggctcagc gggatgagcg
     1201 ttgagccggg gcgtaatact cgttctgcta ctccttcagg gcagccgcca cgcgctctag
     1261 ccgaatgcgg gcctcttcgg caatcggctc gagttcgggg cggcccacga tgcccagcat
     1321 ccccaacggg tccacgatag aaatctcaca cgaagcgtca tcgagttggg ccaccacgac
     1381 gttgcacggc aacagcaggc ctacttcagg cgcggcggtc agcgcgcggt gggccagcgg
     1441 cggattacac gcgcccagaa tcttgtacgg gcgaaagtcc acgccgagtt tcttcttcag
     1501 cgtgtccttc acgtcgatct cggtcagcac gccaaaaccc tcggccttga gcgcgccggt
     1561 gacgcgcgcg acggccgatt caaagtccgc cgcaagacgc accgccaggc cgagcgaggt
     1621 ctgaatccgg tcagtcatag gcatctcctc tttgagcgcg agcgcaggcg acacacaagc
     1681 gcagaatgtg cccgcgtcgc gtataattga tccgaccacc tgtctccgat atgcggagac
     1741 ggccgctttc atgcaatggc cggctcgccg gtgacggtcg taatctcaaa cgccggcggc
     1801 tgctcgaagt gcttcttcac cgcgcattgc tcggctgcgc ggatgaccgc ggccttgtat
     1861 ttttccggga agtccggtgg gaggtgaatg tcaagcagca ccttaccgac cagcccggtg
     1921 aacggatcca cctgcatccg ctggatcagt ctgatgcctt ccgccgaaat gccgcgctgg
     1981 cgacaaaagc ccaggacgta gatgccagcg cacgtgccga tggaagcgag aaacgtggcg
     2041 aacggcgtcg gggccgaacc gtcgccaccg ccttgcgggg gttgatccgt ccgcacggtg
     2101 tacgggccga agtgtgcatc gacccgcgcg ccaccgggaa agtcgatgat catatccatg
     2161 aggtgaatcc tctagtctcc gttcgaggtg ggcgggacat cgctgtgcag cgtgacattc
     2221 acatcgagta tcatcgcgag gcgactccta agcgacccat gcgcctgtgg ctcacgagtt
     2281 gaggcgcgcc cgcacgtcgg cgcctttcaa tggcgcgccg aataacaggg cgccgatcag
     2341 gcacacgtcc agcaagcccg ccagcagcgg caccaaaccg atcacagcca gaataccggc
     2401 gttgttcgcc agtccaatcg caatcagcgc aataccagcg acgatgcgca acccgcgccc
     2461 caatccgctc gacataaact tcgcgaaact catgatatgc ctcccaagcg ttgaattcgg
     2521 ccttcattct attggatgct catcggttga aaaggtgaca accatgccat tgcagcccga
     2581 ctacatcttt cggctgagct ttcccataca tcgaacgacg cgcaagattc cgactcattt
     2641 atcaggacat gtggtcggcc ttggcataga atttatgtgc gacgggcatt cggctggcgt
     2701 cttgcagcgt caacgtatcg cacaccgggg aaccggagtt tggcgaccac cacatctggt
     2761 gctggagtcg aggccggtgt gggccgccaa atatagacat ctagggcagt ttccgattgc
     2821 gtttacgccg ctccggaatc gaggctctag ccggaaaccg cctgaaggct cgactccgga
     2881 aattcgccgg actctgacag ggaagaagga gacaacatga tcgcgagatt gtggcacggc
     2941 gtcacaccgg cgtcgaaggc tgacgtctac ctggacactc tgaaccagac tggagtcggc
     3001 gacctccgcg cgacgccggg caatcgcggc gtgtacgtcc tcagacgaat cgacggagat
     3061 gttgctcatt tcctcttgat ctcgctctgg gactccatgg acgcggtgaa aagcttcgcc
     3121 ggagctgaag tcgacaaggc ccgatactat cccgaggatc gcgagtttct gttagaattc
     3181 gagcccacgg tgacccatta tgatgtgctc gttcgtgaca tgtgaggtcc attgatcctg
     3241 catcacgatg tggtatatgg cccggccgag atcggcgagc cgatcctggt cgaattgatg
     3301 cattcggctg ccgtgcagag actgcacggc gttttgcagc acggcatcag cgggctgatc
     3361 gggatcacgc gccccacgac ccgcttcgag cattcggtcg gagtgatgct gctggtgagg
     3421 cgacttggcg cgtcgctcga agagcaaatc gccgcgcttc tgcacgacgt gagccacacg
     3481 gcgttcagtc acgtgatcga ctacgtgttc ggcgggcacg acagccaaag ttatcacgag
     3541 gaagtgaagg aggaatacct cgccgggaca gacgtcccgg caatcctcgc gcggcaccac
     3601 ttcgactggc acgactttct gcacgaagcg gacttcccgc tgctggagca gcccgcgccg
     3661 gcgctgtgtg cggaccggct ggattacttc ttgcgcgaca ctggcaacct gggtttgtcc
     3721 actgacgacg atgtgcagcg tgccttgaaa catctggtcg tgcatgacgg gcgcatcgcc
     3781 gccgacgatg tcgacgtcgc gcgttggatg ggctatacct acatggccgc cgatcgggcc
     3841 agttgggcca gcttccgcga ggttggcctg tacgagctga ccgcccgtgc cgtcagaacg
     3901 ggactggaaa ccggcgcgat cgccgaagcg gacatctgga gcacggacgg ccagttgtgg
     3961 gcgaagctgc acgccgcaag ccatccgaat cttcaaagcg agctgagcct gatctcgccc
     4021 gacacgcagt tcgtgtggga tgagaccgcg cccacgttcc gggtgagcac caaactgcga
     4081 acgatcgatc ctgacgtcgt gttggacggt cgttcgcagc cgttgtcggc gctggacgcc
     4141 gagtttgctc gacatcgcga cgactacctg cgccgcaacc gaggcaagtg gccgatgcgc
     4201 gtcgtgccag gcgcgggtgg gtgacgcgag atgctcgcga ccccttgggg tgatcaatcc
     4261 gcgatcccca gaccgccgga ttgatcatcc ggcggaagca gggatcgcgc gaagcggagc
     4321 ggggcggcga gccgaattta caagggagag ggaatgacca cgcatagttt atcgggtgtc
     4381 ccagcacggt ggcgcatgct ggtggccttc gcggccattt acacgatttg gggctcgact
     4441 taccttgcga ttcgcttcgc catcgagacg atgcccccat tcctgatggg gggcgcacgc
     4501 tatctgatcg ccggcgccat cctgtacgca ttgacgcggc ggagccgcgc ggcggcggtg
     4561 ccacgcatcc actggcggtc ggccttcatc gtcggcggcc tgctgctgct cggcggcaac
     4621 ggcggcgtca tttgggccga gcaggtgata ccgtccggcc tggcggcgct gctcattgcg
     4681 acggtgcccc tgtggatggc gctgctcaac tggctgagag gcgacaacgt tcgcccgaat
     4741 ctcggcgtga cgctcggcct ggccctgggc ctgctcggca tcgtgttcct ggtgagtccg
     4801 ggtgaatcgg caggcggcga ccaggtgaac tcgctcggcg tgctggtgct cgtgctggcc
     4861 gcactgtcgt ggtctatcgg ctcgctgtac tcgcgcaagg ctcaactgcc agcggagccg
     4921 ctcctggcga cggcgatgga gatgctggcg ggcggggcgc tgctgctcgt cgccgggctg
     4981 ctgaccggag aagcgggcca gcttcgactg gaccaggtat cgccgcgctc gcttctggca
     5041 ttgggatacc tgatcgtctt cggatcgctc gtggggttct cggcttacgt ctggctgctg
     5101 cgcgtcagca cgccggcgcg cgtctccacg tatgccttcg tcaacccggt ggtcgccgtc
     5161 ttcctcggct gggcgctggc aggtgaatcg ctcacgccgc gcacgctcgt tgcggcggcc
     5221 gtgatcgtca cagcagtggt gctgattgtc gccaaccagg ccaggacgct cccgcagcga
     5281 aatgcagacg tgaccggcgt cgaagatcgg gcgtccccat ttcgcagggc cttcgcgctg
     5341 atagcgagtc gggtgcccgg gcgacgtgtg gaagccggcg gcggcaatta gagaagtctc
     5401 ttattcctac atcttttggc cgatgcacgt tttctcccaa gtgccgaacg ccttttccaa
     5461 cgttttcgcc atgacggaca gcctgagatg gtatagaatg cgagaaattc agtgttgagc
     5521 ctcgccgctt gccatggccg gccaggagac cgccggacag tgagttccgg gaagccaact
     5581 ctttaccgga tcggaaaagg acatgatcac aatcttactg gtggatgacc agccggctgt
     5641 tcgtcagggg ctcaacatgc gattggcgct ggagccggat ctggaggtca tcggggaagc
     5701 aacggacggg gtggaagcgc tcagacgagc gcacgagctg cacccggatg tcgtggtgat
     5761 ggacatcgag atgccccgcc tggatgggat cgccgccacc gaggcgctac gcacgatcga
     5821 gccaggctgc tgcgtcgtca ttttgacggt gcacgagaac tctgccacgc gggcgcgcgc
     5881 aaaggacgcc ggcgcggccg cgttcgtcag caagcacgcc ggcgaagaag cgctgctcgc
     5941 ggctattcgg ggcgctaccg gcctggcgaa cgtgagtcaa attgaaattg gccctaatcc
     6001 ttaggcggcg ccgtcaaggg aaaatggacg cggatgcgcg tgccctgacc cggagcgctt
     6061 tctacggaga acgcgccgcc cactttctcg gcgcgctcac gcatcgagcg cagaccgaga
     6121 tgtcccggaa acgcactgtg tgtgtcaaac cccacgccgt tatctccgat ctctaatctc
     6181 accgatccat cgctcgtttc cagttgcaca ttcacctggc tggcgcgcgc gtgcttgacg
     6241 acgttgttca gcgcctcttg cgcgatgcga tacagtgctt ctttcgcggg cagcgccagt
     6301 tgaggctcgc cgcagaatgc cgtacgcacc tcgatgttgt accgggcgcg cagcgaagcg
     6361 gtctgcttgc ccaacgcggc gaccagccct tccatctcga gcgattcggg gcgcagctcg
     6421 aagatcagcg cgcgcatctc ggccaatccc gcttcggata gcgatagaac gtagtcgagc
     6481 ggctcgccca ctttccccgg atcgcgatcg agcagcgtgc gcgcagtgcg cgcgccgagc
     6541 gcgatgccgt acaacgcctg cgagaccgag tcgtgcagct cgcgcgccag cttctgccgt
     6601 tcttccagcg cagccagttg ctgcgcctgc tcgtacaggc gagcattctc gatggcgacg
     6661 gctaactgat tggctaggga ttgcagcacc gtcacgtcac tctcgtcgaa agcgtccgag
     6721 cggtcgctct cgacgtccag cacgccgatg ctctggcccc tggacttgat cggcacggcc
     6781 agctccgaac gcgcccgggt agccgacggt gaagcaatgt atcgaggatc ctggctcacg
     6841 tccggcacca ggagcggttc accggctccg gcgacccagc cgataatgcc ctcctgcccc
     6901 acccgcaggc gcaggggatc gccatacttg acgaccacct cgtccccttc gatcagtccg
     6961 atgctcgtga agtaataccg gaaagtctct tgaatcagtc gggctgtctg agccagtagc
     7021 tcgtccaccg tcagaataga ggtgatgtga cgccccacct cgttaatggc ccggaactgc
     7081 tcggcgcggc gctgctcggt ctcgaacagc cgggcgttct cgatcgcgac agccaactgg
     7141 ttcgccaggg actgaagcac ggtcacgtca ctttcgtcga aggcattcag acgctcgctc
     7201 tcgacgttca acacgccgat cacccgtctc ttcaccttga gagggagcac caattccgag
     7261 ccggtctgac cgtcctgcac aggaatgtag cgcggctcct ggctcacgtc tggcaccagg
     7321 acaggctcgc ccctcccggc ggccaggccc gtgattccgt ccagcccgac ccgcagttgc
     7381 agggagctgc agcaccggca atcaggctca tcccaccaca caccggccgt ggccttatag
     7441 gcgacgacgt ctttctcgat aagcccaatg tgaacgtgat agtaaccaaa agactcacgg
     7501 atcagtctga ccgtctggct gaggatctcg tccacggcca ggatcgacgt gatgcgctgg
     7561 cctacttcgt tgatgacacg aaactgctcg gcacggcgct gcccggcctc aaacaggcgc
     7621 gcattttcca tggcgatagc cgcgtggctg gcgaaggcca cggccagttc cgcgtgccgg
     7681 gacgagaagt tgcccggctg gctgtggtgg acggacagca cgccgactgc cttttcctgg
     7741 acgatcaagg gcacacccag ccaggagcga atgtagctga gagcaccgtc gattcgctcg
     7801 ggctgcagct tccctatcga atgcgtctgc gctgcctcct cgcgcacgtc ggggaggatc
     7861 acgggctgcc gatcagtgac gatctgctgg atcaggctgc cctggagcgg gaactggatg
     7921 tgcagcacgg cgtccatggg agccggcccg cgataagcgc cgtatcggac ccgatcaccc
     7981 tctacgatga gcacggaagc tcccgcgtag tcgacgacgg atttcaattg gtccaggatg
     8041 atcccgagca acggctccag ctcaagcgtc gacgtcacgt cacgcgcgac tttcagcaac
     8101 gtggacagct cgtgcgtgcg ctcctccacg cgctgctcga gcaattgaaa ggcttgtttg
     8161 cgttcggtga tgtcttgcgt catgaccatg ccggcgacga tgtcgccagg ctcattcctg
     8221 atcggcagtg cgttcacctg gtaaatacgg tcggcgaacc ggatttcaaa cacattcggc
     8281 gtgccggcca gcgcggcacg atagtgaggc tccagaatct cgcacgtgtc aggggcgaat
     8341 atctcccaga tcgtcctgcc ctccaacatc tccttggaca atccggtttc ggccagcccc
     8401 gcgccgtcgg cgagggtgta acgcaggtta tggtcgaata gaatcaccgc cccgttgggg
     8461 aagttgcgcg ccagagtgcg gtagcgctcc tcgctctcgc gcagcgcggc ctcagcctga
     8521 cggcgctcga tctcgcgccg ttcgagcgac tcggccatct gatcgaaggc gtgcgccaac
     8581 tggccgatct cgccctgttg gtccgtgcgg gagagcccgc tgcgcgcgcc gagatcgcct
     8641 gcgctcaggc ggcgcgtggc gtgcaccagc gcctgcacgg ggcgcaggat gaatcgatcg
     8701 ccggcgatcc acgccacgcc gaaggcgagc agcgccacca gccctagtgc cgccaggttg
     8761 cggaagagga tggcattacc ctcggcaaag gcctcgcggg cggggatacc gatgctcacg
     8821 gtagcagcgc gcgtatcccc ggtaccgctg ctgagcgggc tgaagccata taggcgcggt
     8881 acgccgtcag cgtcagtcgc ttcaaccgcg ccgccgtccg gctgcgcctg gatcgccgcc
     8941 agaacgagtg tgctcagcgt ctgtccgatg tagtagtcgt caccgggatg gcggatcaga
     9001 atcgtgccct ggcgatcagt gacgatgaat gtggaaccgg ggggcaactg tgcctgcgag
     9061 gcgagctgat tgagccagcc gagatcgagc gaggcaaaga gcaccaatcg cacgcggccg
     9121 ttgaagtcga gtaacggata tccgacgttg aggacggtct gaccctttgc ccggtcgatc
     9181 tcgtaatcgc ctagactgaa gctcagcgtc tgcaccgcat tctgaaagta cgtctgtccg
     9241 gacacatcga gcggaccgct cactgtcacc acgctgcagt acaccctgcc gtttgcgtcg
     9301 atcaagccga ggttggcgta gcgtggcgcg ttctgctgat gctgcgtctg caagctgctg
     9361 aagaccgcgc tgcaagaagg caacccttcg actgcgctca gggcaggccc ggcggcgcgc
     9421 aactccggcc gttcagccag gcggatgagc atctggcgcg cctgctcgat atactgggcc
     9481 tggttactgg cggccaggcg ggcgatttgc agcgcgttgg tgtgagcgag gtctgtcgcg
     9541 aggtcacgcc tctccgcagc gttgtacaag atcaaaccca acagcgggac gatcgccagc
     9601 agaaccagcg ttagcaggcg caggcgcagg ttcgaaggct gtaggttacg cacggcgctc
     9661 cctcacctat cccaacggcc ggccatcagc ggcgtggcgg cgcgaacggc ctcggccatc
     9721 gcctgggccg cgcttgcttc gccgttccac actcgattga tggccggctg aatcacctcg
     9781 ctggagatct gcccaaagtt cttcacgtag tagtgtggcg cgcggacgac gcagcacagt
     9841 gcatagtcga cgatggcctg tcgcgattcc ggcggatgag cggggttatc gatccaggag
     9901 gcaatcgcct gcgggtcggt gtagtatgac aactgcaacg gcatccacag tccctgcgcg
     9961 aagagatcga cgactctggg atcgttgtgg aatttataga atttgatcgc agcctctaga
    10021 tgctgcgtgc cactgaagat cacggtaggg ctgccgaaga tgagcgtctt ggcttccttc
    10081 atcttcggca gcaccgccac accgtaacgc aagccctcca tgctggcgta gtccaggagc
    10141 ttccactggc cgcgaatgtc catcgccagt tggccggtct ggaacagcgc gtcgctgggt
    10201 ggcagccgct cttgttgcgc cggggtcggc gtgacgtgct caacccacat caggtcggcc
    10261 agccgctgga ttgcctcgac ggcttcgggt gagtcgatca tcagccgcgt gccatcctcg
    10321 ttcacgatct caccaccgtt gctgtaaatg aacgggggat agctgtacca tgccgatttc
    10381 acgaacgaga cgccgtacac ctggatgtgg tcaggatcga aaccggattc gccaggatgc
    10441 cggccgtccc gatcgacggt gagccgccgg gccgccgcca ggaactcatc ccatgtccat
    10501 gcgtcttcgg cgcgggccgg cgggtatgat acgccagccg cgtcgaacac gtctttgttg
    10561 tagaacaaca gcatgatctc ggtggcggtg ctggtgccaa tcgtcctgcc tggcccgatg
    10621 gggaagaacg tttcaggcag ccgcgacgac aattcagagt cggcccgcac gatatcggtg
    10681 aggtccatca ccttgccttc gctggcccac tgcggcgcgt gagtatcgag caggtaaccg
    10741 acgtcaggtg gtgtgccggc cgcgatcatc gccgacacac gggcgatgta ctccgagtcg
    10801 gggatgtaca gcgcctcgac ctggatgtcg gggttctcgg cgtggaaggc acgcaccatt
    10861 tgctcgaccg caacccgctc gatgggagag ccccagtagg tgaagcgcaa cgtgacgggc
    10921 cggtcgaccg tgggagtcgg cgtcgatgat gcctgacatg cagccagcgc cgccgcgctc
    10981 gccaccagca cgcttaggct gagaagtagt tttctcatgc ggtcacgcgc tccgcccttc
    11041 tccaccgctc agagcttgcc ctgagccttg cgaaggggca ggccctgcgg attcgatcga
    11101 cgccagtccg atccgtactg cgtacagtgc ggcctgcgtg cggctgggca cgccgagctt
    11161 ggcgagaatg ttgctgacgt gggtcttgac cgtcttctcg ctgagactca gttggtgggc
    11221 gatctccttg ttcgattttc cctgcgccag caggcggagc acgtcggtct cgcgctcagt
    11281 cagcgcctcc gggctgtcgg gagcgcgcac ctcgcgcatt aatcgcgcgg cggctttcgg
    11341 cgacagttgc acctgaccat tggcggcggc tttgatcgcg cggcgcagtt cgtcggcctc
    11401 ggtgtccttc agcaggtagc cgatcgctcc ggcgcgcacg gcgccgatga ccttctcgtc
    11461 ctccaggacg ctggtcagcg cgatgacctc cgtgtcggga agctcgcgcc gaatggccgc
    11521 cgtcgcggcg actccgtcca tgaccggcat cagcatgtcc atcaggacga cgtcgggttt
    11581 cagctcgcgc gccatgcgca cggcctcggc gccgtccgtt gcctcgccga tcacctccag
    11641 ctccggatcg agcgccagga acatgcgcag cccctggcgc acgacgctat gatcgtcaac
    11701 cagcagtatg cgaatgggca tcagggtacc tgcctttcgg gtattgggga ctcgacgtgt
    11761 ccgagtctct tcacgactac cagtgtgaaa tcatctgcct gcggcgcctc gccgctgaat
    11821 ctctggacgg cagccaggat ggcctcgatg acgtcctgag ggacgcccga cgaagcggct
    11881 gccaccgtct ccagcagccg cgcctgccca aacagctcca gatcgatgtt caccgcctcg
    11941 ctgatgccgt cggtgtacag aatcagcaaa tcgtccgggg cgaggtcgat ctggcgctct
    12001 tcgtactcga cgccttcgga cacacccagc accatgcctc ggcccgccag ctcttgaaat
    12061 tgacctgacg cagcctgcca gtgcagcggc cgcgggtggc ctgcgttggc gtaagtcaaa
    12121 tggccactac cggtattcag gatggcgtag aaggcgctga caaaatggct ggtctgattg
    12181 tctttctgaa tcaggtcatt ggcctggctg aaggtcagcg ccgggctggg ctggccgagc
    12241 gcggtagcac ggatgatggc gcggctgtgg gccatgaaca gagccgccgg tgtgctcttg
    12301 ccggagacat cggcgatgag cacgccgatc cgcctccctc cggtcggcgc aagcgcgtcg
    12361 ggaaattcga tgaagtcgta caagtcgccg ccgatcaggc gggccgcctg gtaggcggcc
    12421 gcgaactccc aacccggcac gcgagggcag gactccggca acaagccgag ctggatctgc
    12481 cgggctgcgg ccagatcacc ttccagaccc tgccgttgaa gctcgacctg gtgcagccgc
    12541 gtctgttcga tgatcagggc cgcctggttc gccagcagac gcaggaagcg cacctcgtcg
    12601 tcgtccagca atctcggctc gcgactgtta agtaccagca cgccgatagc ccggccttcc
    12661 gccaggagag ggatcagcgc gtgtccacga aaaccttccg cctgccagca atccggcgcc
    12721 caggacgccg aagtgcgggt gaggtcttct gcctgccagg gccgctggga ccgcatgacc
    12781 cagccgaggc cgttgccctc gtcgaagggc agtcggacgc actcggccac ggggtctcga
    12841 tgccagccgc tggcggccct gaagttgaga taaccgggtt ccctctcagg gagtaacaaa
    12901 gcgcaggcgt cgacgtgcag caggcgcggt atctcctgca ccaggtagtc cgtgagcgcc
    12961 gccggatcag ggcggtcgag caactggttg gagaagtcga gcagcgctgc ctgcgcctgg
    13021 ttcgccaggc gcagcttgtc cagaatatcg ccgatatagg agtgcacgac cggctcgctc
    13081 acgaccagct tggcggcaat ctcctgatcg gacaggccct gcgccgccag gcgcagcacc
    13141 tcgctttcgc gatcggtaag cgtttcgaag gtgtcctggg cgcgcgtctc ggccagcagg
    13201 cgagcggcaa tctgaggcga caactgcgcc cgtcccgccg tggccgcctt gatggcccgg
    13261 cgcaggtcgt cggcctcggt gtctttcaga aggtagccaa tggctccggc gcggatgacc
    13321 gccgtcacag ccgtgttttc caagacgctg gtcagcacga tgactcgggt cggcagcccc
    13381 ttgtcgcgaa tggcggcggt ggcctccagc ccgctcatga ccggcatcat caagtccatg
    13441 agcacgacgt ccggcttcag ctcctcggcc agatgcacgg cttcggcccc gttggcggct
    13501 tcgccgacga cttcaagctc cgagtccaga cccaaaaaca aacgcagccc ttgacgcacc
    13561 acgctatggt cgtcggcgag cagaatccgg atcggcatgg aaccttcttt ccggtagggg
    13621 gatgacctcg tgatgtatgg gcattttacc atcgctcaca ggttgcgcaa gaaccaggca
    13681 gacgagtgtc acgcaaaggt tatcaggcga ggacggcttc atttcatcac cagcggcaaa
    13741 tagattagca ctggcgccgg cgttgatgtt ggagtgggtg aaggcgtgtc agtggccgca
    13801 ggcgacggtg tgctggtcgg cgtcggcgta tccgtgggcg tgggcgaagg cgtgtcggtc
    13861 ggcgtcggtg atggcgtatc cgtgggcctg ggtgtctcga gtccaaacac atacgccgag
    13921 ccttggtcgg cgttagcgcc gacgtcgtcg cggtatgccc caacgcccac cgtgcctccg
    13981 ttgatcgcta caccggagcc gaaggagcca tccgccgcgc cgtcgaaggc ggcaagtttg
    14041 gcgctttggg tcatatccat ccagccccca ccgggcttga caaacacata cgccgagcct
    14101 tgataggtgt tggtgccaat gttgtcgaaa ggtgccccga ccaccaccat gtccccgttg
    14161 atcgctacgg accaaccaaa ccagtcaagc gccgcgccgc cggaggcggt gagtttggcg
    14221 gtctgggtca tctccgccca agccccgccg ggcctgacaa acacatacgc cgagccttgg
    14281 tcggcgttgg tgtcgacgtc gtcgccatat gcaccgacta ccagcgtgtc cccactaatc
    14341 gctacagagg cgccgaacca gtcatccgct gcgccgtcgg aggcggtgag ttgggcggtc
    14401 tgcgtcatat ccgcccaacc cccaccgggc ttgacaaaca catatgccga gccttggtcg
    14461 gcgttggcgt cgatgtcgtc taaaagtgcc ccgaccgcga ctgtgtcccc actgatcgct
    14521 acggagatgc cgaaccggtc aagcgccgcg ccgtcggagg cggtgagttt ggcgatctgg
    14581 gtcatatccg cccaaccctc accgggtttg acgaatacat acgccgagcc ttggttgacg
    14641 ttggcgtcga tgtcgtcgaa aactgctcca accactaccg tgtccccgct gatcgctgcg
    14701 gagatgccga accggtcatt tgccatgccg tcggaggcag taagcttggc agtctgggtc
    14761 atgtctgccc aacccccgcc gggtttgaca aacacatacg ccgagcctgg cacggtgctg
    14821 gggctgccgg tgggaccgcc gggcgctccg accaccaccg tatccccgct aatcgctgcg
    14881 gagtagccga accagtcaag ctccgcgccg tcggaggtgg tgagcttggc agtctgggtc
    14941 atatccgccc agcctccacc tggcttgacg aacacatacg ctgagccctg gtcggcgttg
    15001 gcgtcaatgt cgtcgacagc cgccccgact actactgtgt cgccgctgat cgccatggac
    15061 cagccgaacc cgtcaaccgc cgcgccgtcg gacgcggtga gtttcgcccg ctgcacccac
    15121 aggtctatcg cgagcggata cgccgcgccc gcatcctcca cgtgcaccaa cactcgcgcc
    15181 tcacgcacct ccagccaccc cggcaagcgc cgccccgtcg cgtcatgcgc caacaggccc
    15241 ttgtagcgca gcaccgcctg cccatcagcc tgcgccagcg tcagcgttgc accgtcggcg
    15301 tccacccgcg cctgcagccc cgccggtagc cccaacgcca gggtcagcgg tgaactgcct
    15361 gcacgttcag cacctgcggg cggcgcaaga agcgtaaagc cctgctgcaa acctaatggt
    15421 ccgttcacgt accactcagt cgcttcccca cgcggataca ctacgcggtt ttcctgcgcc
    15481 cgtggttgaa cgggcgccac aggctccagc gcctcgccgt agccatagcc cagcagcgcc
    15541 agtctccagc gcacttcgcc cacacgcacc tgagcgcctt cacgggtaaa gtccgcttcg
    15601 agtgcccggg tcggatgggc cgcccggaag cctgcttgtg tggggatgac gtgatagtct
    15661 ttgtcgtcgc gccccaggac gcgtgagatg gcgaactggg cgtccagggg caggctcgcc
    15721 aggccctggc cggcggccgg ttgttgggct gcctcttgtg accccaggct ccaactgccg
    15781 gtcagcagta agagcagggt cagggccgcc agtcgtaaag agatggagac gagagggaaa
    15841 tgagtggtca ttcttgagat tacgagccac gcggattata ctatggccgc ccgaggacac
    15901 cggcatcgtg cccataagcg aggagacatg ccatgatcgc ttcgcgagtg ctgctcatca
    15961 tccacaatcc ggtcgtcgat ccgcagcagg gcgccaagct ctcccaggcg ctggattgga
    16021 acgacccaga cgcgctggcc gaccagtaca tccgcgacgt gcgacaggcc agtcacggca
    16081 tcgtcgacta tcagatcgtc gagcgcgtcg aagtcgacga ctttccggcg aaaatcgacg
    16141 gcttccgcta cgacgctgcg tcgtacctgc gctgcatacg cgagcagcgt ggttttcacg
    16201 agcctgacgc gatcgattac cttgcgctca tccgcgaatt ccgcatgatc gagaaggtcg
    16261 cggctaacca gatcgacgag gtgtggctga tcggctttcc gtactccggc tactacgagt
    16321 cgaccatgtg cgggcgcgat gcgttctggt gcaattcgcc gccggtgaag ggcacggagc
    16381 acagcgagcg gcggttcgtc atcatgggtt tcagctacga gcgcgggatg ggcgaaatgc
    16441 tggaagacct gggccaccgc gcggaagcga tcatgacgaa ggtgtacgaa ggcgtgaccg
    16501 gcgagcgtaa cctgtgggag cgcttctcgc gctacgacag tatcgcgccc gggcgcgcct
    16561 cggccggcaa cgtgcacttc gccccgaaca gcgagcgcga ctacgattgg ggcaatccgc
    16621 gccccgtgtc gagcgactgc gacgactggc tgaactttcc cagcctgacc ggcgcgcggc
    16681 gcatggtcga ctgccgcgac tggggcaacg gcgacatacg cctgcaccat ctgtggtggc
    16741 tcgaacgcct gccacacgcc gagggcgaga cggacggcgt ccgcaacaac tggtggacgt
    16801 acgtcgtcga cccgaacacc gttcaaaaca gcggatgtct cacgctcttc ctgagctggt
    16861 ttcagcgcct gagcacacct agatcgcata ggactgcata tcacgcctaa cccacccatg
    16921 tccagcctgt ctttcgaccg cgccgccccg atttacgaca gcacgcacga gctgccagcc
    16981 gagatttccg agcgcgccac gcagacgatt ctcggcctcg cgccatctgg cctgccgatc
    17041 ctcgaagcgg gcgtcggaac cgggcggatc gcagtcccgt tgatgcggag cggcgcgccg
    17101 ctcgtcggcg tcgatttgtc gctcgacatg atgcagcgcc tgcgggcgaa gcacggcgcc
    17161 gcgtcgctcg cgcaagccga cgcttcacgc ctgcccttcc ccgacgcgac catcggcgcc
    17221 gtcctcaccg tgcacgtgct gcacctcgtc ggcccctggc gcgaggcgct gcgtgagttc
    17281 aggcgcgtct tgtggccggg cggcgtttat ctcaactcgc acaactaccg gcacgcggac
    17341 tcgcccaacc ggcggctgcg cgatcgatgg catgcgctgg tggagacgcg cggctggacc
    17401 tggcgccgtc ccggcatcca ggatcgcgaa gagctgatag aggagctgcg cgcaatgggc
    17461 gcccaggtcg aagaggtcac tatcgccgaa tggacaggca gcgtgacgcc gaggcaggag
    17521 ctatccgaca tcgccgcgcg cacgcactcc gatacctggc agatccccga tgacgccctg
    17581 gctgaaaccg tcgccgagct gaccgcctgg gccgctacgc agtatgacga tctcgacgcc
    17641 ccgattgccg tcaagcggcg tgttggattc gacgtcatcc atttttgaga gccggagttt
    17701 ggcgaccgcc atatctggct ttggagtcga gacattagcc ggatttgacc gccagatatg
    17761 accgcctaaa ggcctgatct ttacccacat ttgtcagctg gtgtcgggag cgcggaacag
    17821 gcgcgccccc tccgtcaggt cggataggag gcgccagccg cgccacacgg tgcgccagcc
    17881 cggcgcgcca tcgcgg
//
LOCUS       DBNHXB010000060         5659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3535076, whole genome shotgun sequence.
ACCESSION   DBNHXB010000060 DBNHXB010000000
VERSION     DBNHXB010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3535076"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..1416)
                     /locus_tag="AC162G_02250"
     CDS             complement(178..1416)
                     /locus_tag="AC162G_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase
                     non-catalytic subunit"
                     /protein_id="HIR5074666.1"
                     /translation="MKSLDDVIRTIIRLREAGVVQITLLAVCPNSAAVLEAAVKVAAL
                     THMPMLFAATLNQVDRDGGYTGWTPAQFVAEMRAFAGKYHCTSPLYPCLDHGGPWLKD
                     RHTLDGLSYEATMSEVKRSLTAGLEAGYQLLHIDPTVDRRLPLGQPVPVQDVVDRTIE
                     LIAHAEAERQRLGLQPVAFEVGTEEVHGGLVDFGTFESFIKSLHAGLEAHGLSHAWPC
                     FVVAQVGTDLHTTFFDAEAARRLASIVAPMGALLKGHYTDWVENPADYPASGMGGANV
                     GPELTSEEYLALRDLEAKEQALWRNRHNIEPSRFMRALEKAVVDSGRWKKWLQPEERG
                     LDFAALSGERRSWLAQTGARYVWTHPDVVEARRTLYSNLEVVMPDPHAHVVERIARAI
                     DKYVLAFNLFDSLALFEGIS"
     gene            complement(1520..2014)
                     /locus_tag="AC162G_02255"
     CDS             complement(1520..2014)
                     /locus_tag="AC162G_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074667.1"
                     /translation="MLKRTTKTFVLVLLAEVVIVAGIVLVSQDMAMREYPPLVGAAAP
                     VVGFALLAAPIVLAVVLPGIAPRWTTRVLREGRQAPAIVLANDVLKGIGGYEGENAWV
                     ELPARVQPEGEPPFEASVKCRLSQSLALSAGTQVAVKYDARDRRKVVLVGDLPALFKA
                     RVNT"
     gene            complement(2069..3043)
                     /locus_tag="AC162G_02260"
     CDS             complement(2069..3043)
                     /locus_tag="AC162G_02260"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR5074668.1"
                     /translation="MPLDVVVIGPLNVDLLITGSAPTDMDKLTRWAGPSNVTLAAAGS
                     AGYIVQDLVRLGLRAGVVSVLADDPFGDAVLRILRESGVDATHVQREPDTLSGIGIYM
                     LLFGSKKRPLTYRLPTHRPWPAPFAPGDLDYLFGARHLHCAGYLHFPDMWSDRMADLF
                     RQAKARGLSTSLDPQFVLFPVDTPWMEPLVDLLRFTDLLMLDEDEARQVAQADDLRVA
                     ARVLREAGPPVVVIKRGAQGSLVCAGERIFEQAAVMVPEEEIVESIGAGDAFDAGLIV
                     GYLAGWPIQRSTQFATLAAASTLRGSGGAQSLASHAELERALQSPQGD"
     gene            complement(3126..4370)
                     /locus_tag="AC162G_02265"
     CDS             complement(3126..4370)
                     /locus_tag="AC162G_02265"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR5074669.1"
                     /translation="MDESRLRETGYRVKRWGIDDAKASRLAEATSEYPIMYQALGTDP
                     KTSHPFNLYYDIRAQPKALADTLVQNERLIASIAEEMIRRDITRVVGSGLGTSQFVGQ
                     VAGNGYWKFAGIDADQVDAVEFQLYERPYDWAKTAFLAYSGSGSTTDSNRAVQIAKER
                     GAYIVALTSIAGSPLSKIADVNIVCAGGYDTGGSDTFHYTTRLAASLMMALEIGERAR
                     PGAHDYAALKRQLREAGQRMGEVFEQVDARCRSIAKRLKRARAVLVVGSGPHLGTAEE
                     IALKFDEMCHIPAKAMCWGRHIHGALGLTDERIHTIIVAPPGEAYTRLIDIARVTQML
                     KTPSVAIVTEDDEEIASRVDDVVRLPGMEETLFGVMAILPGQLLPYYCAVELGDVNPD
                     CQRSDIPKHARVWHMMFPPGTH"
     gene            complement(4548..>5659)
                     /locus_tag="AC162G_02270"
     CDS             complement(4548..>5659)
                     /locus_tag="AC162G_02270"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR5074670.1"
                     /translation="VERLKVGEKQIVEIAKALLDQSRLLIMDEPTAALNSAEAEALFA
                     IIATLKARGVTILYVSHRLNEIFRLADAVTVLRDGRHIRTCTIGDVTPDSLITDMIGR
                     KLEGVFPPRNPALGERALSAEGLSAAGMFEGVSFELRAGEVLAVTGLTGSGKTELGKA
                     LFGDWPVDSGEVRLFGQADRAIPARAIQAGLGYMPEDRKVEGLLAEVSVRRNVALAIL
                     PRLARRLGILDRPAEHKAAQAQVDTLEIKTPSLDQLVRNLSGGNQQKVALAKWLACGA
                     RALILMEPTQGIDVGVKFEIYELIARLSHEGVAILLISSELPEILGLAHRILVMRAGH
                     VVAELPGDQTNAEAILRYALGESVDRSDQSSVISN"
BASE COUNT          991 a         1900 c         1752 g         1016 t
ORIGIN      
        1 ccgcgatggc gcgccgggct ggcgcaccgt gtggcgcggc tggcgcctcc tatccgacct
       61 gacggagggg gcgcgcctgt tccgcgctcc cgacaccagc tgacaaatgt gggtaaagat
      121 caggcctaaa ggctcgactc cggaacttcg ccagacttca gtggagcaga gtagagatca
      181 cgagatccct tcgaacagcg cgagtgaatc gaacaggttg aaggcgagga cgtacttgtc
      241 gatcgcccgc gcgatgcgct cgacgacatg cgcgtgcgga tcgggcatga cgacctctag
      301 attcgagtac agcgtgcgcc gcgcctcgac gacatcggga tgcgtccaga cgtagcgtgc
      361 gccggtctgc gccagccacg agcggcgctc gccggagagc gcggcgaaat cgagcccgcg
      421 ctcctcgggt tgcagccact tcttccagcg acccgaatcg acgactgctt tctccagcgc
      481 gcgcatgaaa cgcgacggct cgatgttgtg tcggttgcgc cacagcgcct gttccttcgc
      541 ttccagatcg cgcagcgcca ggtactcctc cgaggtgagc tccggcccga cgttcgcgcc
      601 acccatgccg ctcgccggat agtcggcggg gttctccacc cagtctgtgt aatgcccctt
      661 gagcagcgcc cccatcggcg cgacgatcga cgcgagccgc cgagcggctt cggcgtcgaa
      721 gaaggtcgtg tgcagatcgg tgccgacctg cgcgacgacg aaacacggcc acgcgtgcga
      781 caaaccgtgt gcctccagcc ctgcatgcag gctcttgata aaggactcga acgtgccgaa
      841 gtcgacgagg ccgccgtgca cttcctccgt gccgacctcg aaggcgaccg gctgcaggcc
      901 aagccgctgc cgctccgctt cggcgtgcgc gatcaactcg atcgtccgat cgacgacatc
      961 ctgcaccggc acgggctggc cgagcggcag gcggcgatcg accgtcgggt cgatgtgcag
     1021 cagttggtag cccgcttcga ggccggcagt caggctgcgc ttcacttctg acatcgtcgc
     1081 ttcgtacgac aggccgtcga gcgtgtgccg atctttcagc cacggcccgc cgtgatcgag
     1141 gcacgggtac aacgggcttg tgcagtgata tttcccggcg aaagcgcgca tctcggcgac
     1201 gaactgcgca ggcgtccagc ctgtatagcc gccatcccga tcgacctggt tcagcgtggc
     1261 tgcgaacagc atcggcatat gggtgagcgc ggcgactttc accgccgctt cgagcacagc
     1321 agccgagttc gggcataccg ccagcagcgt gatctggact acgccggctt cacgcaggcg
     1381 gatgatcgtt cgaatcacat cgtccaggct cttcaccgtt ctattttgcc ctaacgcgga
     1441 aatccagcac gtcgccggtc ccggcgtcga tgatggcgat gtcgcccagg tagcagcaat
     1501 cgaggaacac ccacaccact cacgtgttca cgcgcgcttt gaagagcgcc ggaaggtcgc
     1561 ctaccagcac aaccttgcgc ctgtcgcgcg catcgtattt cacggccacc tgcgttccgg
     1621 cgctcaacgc caatgattga gacaggcggc atttcacgct ggcctcgaac ggcggctcgc
     1681 cctcgggctg aacccgcgcg ggcagttcca cccacgcatt ctcgccctcg taaccgccga
     1741 tacctttcag cacatcgttg gcaaggacga tggcgggcgc ctgtcggccc tcccgcagca
     1801 ctcgcgttgt ccagcgtgga gcaattccgg gcagcacgac agcgaggacg ataggggccg
     1861 ccagcagcgc gaagcccact accggcgccg ccgcgccgac gagcggcgga tattcgcgca
     1921 tcgccatgtc ttgagacacc agcacgatgc cggcgacgat gacgacttcg gcgagcagaa
     1981 cgaggacgaa ggttttggtc gttcgcttga gcataattaa tccttattcg tgttcattcg
     2041 tgcagattcg tggatcgatg ccttgcgctt aatcgccttg gggactttgc agcgcgcgct
     2101 caagctccgc gtgcgaggcc agactctgcg cgccgcccga tccgcgcagc gtcgaagcgg
     2161 cggcgagcgt ggcgaactgc gtgctgcgct ggatcggcca accggccaaa taaccgacga
     2221 tcagcccggc gtcgaaagcg tcgcccgcgc cgatcgactc gacgatttct tcttccggca
     2281 ccataaccgc cgcctgctcg aagatgcgtt cgccggcaca tacgagcgat ccctgtgcgc
     2341 cgcgtttgat caccaccacg ggcgggccgg cttcgcgcag cacgcgggcg gcgacgcgca
     2401 aatcgtccgc ctgcgccacc tgccgcgctt cgtcttcgtc gagcatcaag agatcggtga
     2461 acctgagtaa gtcgacgagc ggctccatcc acggcgtatc gacggggaag agcacgaact
     2521 gcggatcgag cgaggtcgac agcccgcgcg ctttcgcctg ccggaacagg tcggccatgc
     2581 ggtcgctcca catgtcgggg aaatgtagat agcctgcgca atgaagatgc cgcgcgccga
     2641 acaggtagtc caggtcgccg ggcgcgaacg gcgccggcca tggccggtgt gtcggaaggc
     2701 gatacgtcag cggccgcttc ttgctgccga acagcagcat gtagataccg atgccggata
     2761 gcgtgtccgg ctcgcgctgc acgtgggtcg cgtcgacgcc cgactcgcgc aggatacgca
     2821 gcacggcgtc gccgaacgga tcgtcggcga gcaccgaaac cacgccggcg cgcaggccga
     2881 gcctgaccag gtcctggacg atgtagcccg ccgatccggc ggcggccagc gtgacgttcg
     2941 acgggcctgc ccagcgcgtg agcttgtcca tgtctgtcgg cgcgctgccc gtgatgagca
     3001 gatcgacatt gagcggtccg atcacaacga cgtcgagagg cataggcaca cctcacgaag
     3061 taatcagtga tcagtaatca gtgatcagtg gttactggtt actggtcact ggtcactgat
     3121 cattgtcagt gcgttccggg cgggaacatc atatgccaca cgcgggcgtg cttggggatg
     3181 tcactgcgct ggcagtcggg attcacgtcg cctagctcga ccgcgcagta atacggcagc
     3241 aactgtcccg gcaggatggc catcacgccg aacagcgtct cctccatccc gggcagacgc
     3301 accacgtcgt cgacgcgcga ggcgatctct tcgtcgtctt cggtcacgat cgcgaccgac
     3361 ggcgtcttca gcatctgcgt cacgcgggcg atgtcgatca ggcgcgtgta ggcttcgccc
     3421 ggcggcgcga cgatgatcgt gtgtatgcgc tcgtcggtca ggcccagcgc accgtggata
     3481 tgccggcccc agcacatggc cttcgccggg atgtgacaca tctcgtcgaa cttgagcgcg
     3541 atctcctccg ccgtgcccag gtgcggcccg ctgccgacga ccagcacggc ccgcgcgcgt
     3601 ttcaggcgct tcgcaatcga gcggcagcgc gcatcgacct gctcgaagac ttcgcccatg
     3661 cgctgtcccg cctcgcgcaa ctgccgcttg agcgcggcgt agtcatgagc gccgggccgc
     3721 gcgcgctcgc caatctccag cgccatcatc aacgaggcgg ccagccgcgt tgtgtaatgg
     3781 aacgtatccg agccgccggt gtcgtatccg cctgcgcaca cgatattcac atcggctatc
     3841 ttgctgagcg ggctgcctgc gatcgaagtc aaggcgacga tgtacgcgcc gcgctccttg
     3901 gcaatctgca ccgcgcggtt cgaatcggtc gtcgagccgc tgccggagta ggccaggaaa
     3961 gccgttttcg cccaatcgta cggccgctcg tacagttgaa actcgaccgc gtccacctga
     4021 tcggcgtcga tgccggcgaa tttccagtag ccgttgcccg ctacctgccc gacgaactgc
     4081 gacgtgccta atcccgatcc cacgacgcgc gtgatgtcgc gccggatcat ctcctcggcg
     4141 atcgaggcga tcaatctctc gttctgaacg agcgtatcgg ctagcgcctt gggttgcgcg
     4201 cggatgtcat agtacaggtt gaatgggtgg ctcgtcttcg gatcggtgcc gagcgcctga
     4261 tacatgatcg ggtattccga cgtcgcctcg gccagccggc tcgccttcgc gtcgtcgatt
     4321 ccccagcgct tcacgcggta cccggtctca cgcagtctgc tttcgtccat gaatgtctct
     4381 cctcgttcgc agttcgcaga tggcttatcg catatcgcgt atcgcctatc gcctatcgcg
     4441 tatcgcttgt cgcatatcgc cgatggctaa tcgcacatgg ctaatcacgc acaacttacc
     4501 aatcaccaat tactgatcac tgatcactga tcactgatca ctgatcacta attactgatt
     4561 acggatgact gatcactgcg atccacactc tcccccagcg cgtagcgcaa tatcgcctcc
     4621 gcgttcgtct ggtcacccgg cagctcggcg accacatggc cggcgcgcat gacgagaata
     4681 cggtgcgcca ggccgaggat ctcgggaagt tcggacgaga tgagcaggat ggcgacgcct
     4741 tcgtgcgaca agcgtgcgat caactcgtaa atctcgaact tcacgccgac gtcgatgccc
     4801 tgcgtcggct ccatcaggat cagggcacgc gcgccgcagg cgagccactt ggcaagcgcc
     4861 actttctgct ggttgccgcc actgaggttg cggacgagct gatcgagcga gggcgtcttg
     4921 atctccagcg tgtccacctg cgcctgcgcc gccttgtgct cggccggccg gtcgaggatg
     4981 cccagccgcc gcgccaggcg cggcaggatc gccagcgcga cattgcggcg cacggaaacc
     5041 tcggccagca ggccctcgac tttgcgatct tcgggcatat atcccaagcc cgcctggatc
     5101 gcacgcgccg ggatcgcgcg gtccgcctgt ccgaacagcc gcacctcgcc cgaatcgacc
     5161 ggccagtcgc cgaacagcgc cttgcccagc tcggtcttgc ccgacccggt cagcccggtg
     5221 acggcgagca cctcgcccgc gcgcagctcg aacgatacgc cctcgaacat gccggctgca
     5281 gataacccct cggccgatag cgcccgttcg ccaagcgccg ggttgcgcgg tgggaagact
     5341 ccttccagtt tgcgaccgat catgtcggtg atcaacgaat cgggtgtcac gtcgccaatc
     5401 gtacacgtgc ggatgtgtcg cccgtcgcgc agcacggtga cggcgtcggc caggcggaag
     5461 atctcgttca ggcggtgcga gacgtacaga atcgtcacac cgcgcgcttt gagtgtggcg
     5521 atgatggcga acagcgcctc ggcttcggca ctgttcagcg cggcggtcgg ctcgtccatg
     5581 atcagcaggc ggctttggtc gagcagcgcc ttcgcaattt cgacgatctg cttttcccct
     5641 accttcagcc gttcgacgg
//
LOCUS       DBNHXB010000061        16658 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2864073, whole genome shotgun sequence.
ACCESSION   DBNHXB010000061 DBNHXB010000000
VERSION     DBNHXB010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 16658)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16658)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16658
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2864073"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..933
                     /locus_tag="AC162G_02275"
     CDS             193..933
                     /locus_tag="AC162G_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074671.1"
                     /translation="MNEQLLGYLFDDKPHILAAPMSRWIGLSPRFKAFATTYRNKIRK
                     RIRVTQSGAGIKDLEVELETAYWLLQEQRFAVAYEPYSGEKTRGPDFAVTFKSITFNV
                     EVTRIGSWEGERPLPAPEGVTTPWLDPQQVGGRLTDTVCDKLGQMRPGMINVLIVTTG
                     SDFIRVLELNQAMARLKERAERKEAGLFGRYGFVNTADFFKYYLRLSGVLVRHVGKQD
                     EAADSILWANNQAKHPIPSRIRTILQRG"
     gene            1176..3959
                     /locus_tag="AC162G_02280"
     CDS             1176..3959
                     /locus_tag="AC162G_02280"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5074672.1"
                     /translation="MPDTILKTKLYIPPARSKLVLRPHLISRLSEGLAGKLMLVSAPA
                     GSGKTTLVSEWIHRLAGEDQAVASAFPAVAWLSLDEDDNDVARFWSYVIAALDTIRPG
                     VGTDALAMLRSAQPPPYQTILTALINDLDDRPDPLALILDDYHAIHVQPIHDALAYLL
                     DHLPPHTHLIIATRADPPLPLSSLRARRQLVEVRADDLRFTPGEIAAFLNQIMGLGLS
                     ADDLRALEQRTEGWAAGLQLAALSLQEEEDVAGFIRAFTGSHRYVFDYLAQEVLERQP
                     DSVRTFLMRTSILERLSGSLCDALTERTDGQSTLERLEQANLFIVPLDQSRRWYRYHP
                     LFADFLRAQLQQAIGAHGILALHLQASEWHEQNGSIRDAVSHALAADDHTRAARLIED
                     YAPAMFLQSELTTLTGWIHRLPVEDVYARPRLCMANAWALLATGHGEEAERWLQAIER
                     TLGASAEGLADEDSGRLSPDVLGALVEVTALRGVLAVGRFDVPRTLELSRRVLRHLGD
                     SELRGLFNDPPQMRPAALFNLGLAQELGGEVGTAAGTFRETAAASLELKNQHLYPLAL
                     GHLGQLQVVQGQLHQAQLTYRSALQETQEFAGSPSPLSAIAHAGLGNVHYEWNDRDAA
                     LSHLLKGIELAKPWINWESLIPAYLGLARLKRSRGDLDGALTVLDEFIAMCRQSNAAM
                     TSAAQAYQAVLWAARGDSALATQWLREAGIDPAGDLAYGVEFEAIASAQVLIALGRAA
                     EAARLLERLRQSAEAGHRTGRVIEISVWQSVAQHAQAKAPEALSVLERAVTLAAPEGY
                     CRTFVDAGASLAELLAKLLTTRRLDLDETSSTYIRRLLDAMRVDREGKGAAAVRAQPS
                     KSPPAGMAEALSDREMEVLRRLADGLTNAEIGQALVLSVNTVKTHVGNIYGKLGARNR
                     AQAIARARELDLL"
     gene            4123..5040
                     /locus_tag="AC162G_02285"
     CDS             4123..5040
                     /locus_tag="AC162G_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5074673.1"
                     /translation="MNETLVTVQDFRKVYGDIVAVDGISFQARRGEIFGLLGPNGAGK
                     TSTLECLEGLRRADGGRLDIMGVDPGRDTRKLQNLIGVQLQTSGLPADMSVVEAMKFF
                     CAYHGAAPRFDLLERLGLGEKLRSTYHELSTGQQRRLALALAVAHQPPIVILDEPTAG
                     LDVASRVELHAVMQELRDSGSSILLATHDMAEAEKMTDRVAILLKGRIVATGSPRELT
                     ATGAGFTKVSVRSENAALSRNNVSFPAVGQHVFKDEYAIYFSTDPAPTVSAILEYLAA
                     HDDKLIDLRVERPSLEERFLEITHDGGAR"
     gene            5037..5849
                     /locus_tag="AC162G_02290"
     CDS             5037..5849
                     /locus_tag="AC162G_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5074674.1"
                     /translation="MTAFVNHFAFEFKTGLRNPTQMLMNYLFPLAFYALMGLVMTQIN
                     PGFKETLLPAMVIFAVLVSTLLGMPGPLVESREAGIFRSFKINGVPALSILSIPAFTT
                     IFHALIVSVLIALTASSLFGGAAPVDWGSFVLVTLVTAFSCGGLGALIGVIASSARST
                     VLWSQLIFLPSMLIGGLMVPISLLPESIRPVSALLPSTHAMQAFLGLAYRQETLVDPL
                     ASLLILAVSGVVAFGLAIYLFNWDSRNDSRRGHPLMALLAVLPYLVGIFFLK"
     gene            5933..6478
                     /locus_tag="AC162G_02295"
     CDS             5933..6478
                     /locus_tag="AC162G_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5074675.1"
                     /translation="MNVSSPHAPDVSRAQFRARLNRVLSHRLALLIAPRGSGKTTALR
                     DWRHSCGCLTAWVSLSEHHNVPERFIRDLVETLSHAAPTLHARSIWESDGTLTSLKEA
                     MARLINALADVSDTLILILDDYDVIRAAPIHEAMAMLLDYMPPALHVIIASGEDPPLP
                     LARLRVRMQLVSLGIEDLQMT"
     gene            complement(6844..10554)
                     /locus_tag="AC162G_02300"
     CDS             complement(6844..10554)
                     /locus_tag="AC162G_02300"
                     /inference="COORDINATES: protein motif:HMM:NF040218.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HIR5074676.1"
                     /translation="MNTQRLSRLLLSGLRLLLLTTWVLGLWLPGETRLDIGTAYAGTG
                     PTLNILQGGGESTMDGRCIEYSVYSYVQIVVPLEFTVYTFKVYYKHTADYLYFCFNNP
                     YYSPDGPSDVKVYVDRDDDGGAADGNDFVIFIQPDGNPGAAYWNPATSAFNGPDPGGW
                     SAVSRTYTVLGQVRWDAEFQISRHTMGGWKHTAGLSLVHHYVDASGFDFTSSWPNSSS
                     EMDPTSWGNASLKTGEVTIGQSDVVPTVDGNCPATSGFEYADAASVSFSSALAAITAY
                     VKHTSTDLYVCMAGLTVPAIALQNSRNAVVYINRAGTGGDSPGPDDMAFTISYSGIVS
                     VHRGSGSGFDGPDPGGYSIARTTVGGELPQWSAEFRISGATLGGGWARDIDITFAEQE
                     VNATGDYAGWPGGSRWNIPNTWGVGHLTTSPLPAGADMRPTAIEVTQSVQDINNSVVL
                     VASKRTFARVHVASNANRTGVTARLLGFRNSIPLGVLTPINLGGVINVVTSPDRAQLN
                     DSFLFELPANWIVPGTITLEAEINPFHGIREDNYDNNTVTTGDLTFRATNPLILRDVN
                     YEYFQSNGALVSAPTFDQDMLESQLRRMYPISSLSVLRRTLIHFGPFVGFTNQFPFIT
                     IDDQPNAVIVIAELLALRSIVEPANLAAVYYGMVYDRGNMSGFMRGLGFGFPIGTAAG
                     PTGPSSWGWDFDGSYGDWYGSHEIGHTLWRFHTDACNTDGPYQSYPYPNGIIGGPDGN
                     TSRFFGFDVGVGVANLPIRVMPGDTWTDIMSYCDNEWISDVTYTGIRDYLQIGWPLAA
                     GHSDRNIAALNIRSSQPYTTGDFLAVYGVINFTDQVAALPYLSRQSQVSQIPPLIAGP
                     YHIRLLNASHVQLADYAFTPLANSEDTNLTAKTGVIAQTVNFMPGTRRVAIFSDVVNR
                     EIASVNVSANAPTVTITARSGGPSLAASQPVTLTWHGADLDGDALRYTLLYSFNGRTT
                     WRALASGIASTTIAVDSGQLEGTSGASTGYFRVVANDGVLTGSADSDSFSVAGKAPLV
                     AIDNPAHGSVYAYGQTVALEGYGQDFEDGTLGDAYLSWSSSLDGPLGIGHLLHTSLLS
                     VGTHAITLTATDSNGLTGGATVVINIAADVPQPNPMLSVAPDSLLFLVQPGGPNPGPH
                     TLSIRNLGTGSINWTATSDATWATLSSASGTAPSDVTVNVDARALPGGPTRVAHIVIT
                     GAGASNSPQLITVVAQNVTGQASVYLPLVIKE"
     gene            complement(10705..10941)
                     /locus_tag="AC162G_02305"
     CDS             complement(10705..10941)
                     /locus_tag="AC162G_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074677.1"
                     /translation="MKQVTLSDAPRPRTHLFTVRVWLEDLGDGKAEWRGQVKHVMSGE
                     VRYFREWQTLLGHLKAVLARAEKVKEETPENKGA"
     gene            complement(10943..11785)
                     /locus_tag="AC162G_02310"
     CDS             complement(10943..11785)
                     /locus_tag="AC162G_02310"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HIR5074678.1"
                     /translation="MKMNRLTQSPMGIVRSLAILALIPQALTPMHSAFALDNVDTTHL
                     GVGTLVVRLLSGELFPDCSGTLIAPQVFLTAGHCTDAVESWLNAGLIQTAYVSFDSPF
                     TQSASLVNITDLVTHPDYSPAVYNINSDTHDLGVVLLATPVYDRPITRLPSASLLDSL
                     PLKPGTPVTAVGYGIDSNDVHGAPSPGFGYTDTGFRASGTLSYRALAPAFIMVTQLAN
                     QGSDNMCFGDSGGPDFVQVNGQEQLVAINVVVNSYSCEEVAWLYRLDTPQARAFLGQY
                     VALP"
     gene            complement(11782..12948)
                     /locus_tag="AC162G_02315"
     CDS             complement(11782..12948)
                     /locus_tag="AC162G_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074679.1"
                     /translation="MAWNGSREVAFILADVPDFPRPPAEWERRVRERTFYDPVTGADL
                     RAFIRAVSYGRARLTGDIYGPFNVALARNPDGSVHLGVTMDNAIRAAQGLIEGYLYAC
                     IVFTQSPGPAWAFWGYSPGYFYSPQHQQYPSTYTSTITGTCYVDTDDPLGVWAMENLH
                     AITQFGDLYGISDSPGAFDVMDCSCGTHPSSFTKSKLGWLDGSEVVVIPPGTPARTVT
                     LQALGSPRRPGSVYAAKLTSTTGSQRYFLVEARMRVDRYENATPGVSTGISSEGVVVY
                     WIDESSWPPVHLRRPVLTPGAEFADQVEGVRISEVAQAPSGFAVRIERGEPAECDWIR
                     TQLDDLGTRIDDLRQDLVEEADPRVRAGILQEIRALESQAQRLRNRANTLGCSL"
     gene            complement(13156..16020)
                     /locus_tag="AC162G_02320"
     CDS             complement(13156..16020)
                     /locus_tag="AC162G_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013124.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR5074680.1"
                     /translation="MPTLHIRLLGEFSLVYGDELLRTVHTARLKSVLAYLLLHLDAPQ
                     SRQHLAFLFWPDSTEVQAHNNLRQSIHRLRQALPDAESFLHSDAQTVQWLPDSPFTLD
                     VDEFEKAIQQAQSPESLQEAVNLYRGELLPGCYDDWILPERERLKQEFCAVLERLSLH
                     LENLGDYRSATRYTEHLLRSDPLREETYRRLIRLYALSGDRTGVVRVYQTCVRVLKRE
                     LDVEPSPETQAIYAESKSVQAPPARFALSPQRQMNNLPTYLTSFIGREEELEDLKRIL
                     SPQGISAPVTHLLTLTGAGGCGKTRLAIQVASHLAHEFADGVWFVDVATLTDPALIPP
                     AIASILGIQEQPGRDPLDTIVNRLRSKELLLVFDNCEYMVSPCARIVESLLHSCSHLR
                     ILTTSRERLDVIGERVWPVSPLSLPDVNDLSTAALSRSDAIRLFVERAGAAWPTFTLN
                     ADNVAPIAQICQRLDGLPLAIELAAARLTMLTPSQIAARLDNAFQLLAQTSPSIPRRH
                     HTLRATMDWSYELLSEKERTLFRRLSVFMGSFALETVEAVCADREEQGSLSISEILDL
                     LSRLIDKSLVVVSDWRQGGQSRYRLLEPTWQYANEKLLQSSESERLRLRHLEFFLNLA
                     EEAEPHFSHTEQVMWFNQLMADHDNLMSALDWSLGRGELIAALRLTGALWYLWLARGY
                     YTEGVRRLMQAISLTKGAPPSSAQAKALWAAGAIGLWSEGDWSRARPFLEEAVAVSRE
                     LGDKTILAGSLGTLGATAFSQGDYTAARSFLTESLALLRELDDKHATGWSLTYLGDLS
                     RAQHDDEQAHRLYAESVAHFRAIGDINSAGYPIRRLGVMALNRGDYQQAIEWLKESLA
                     LNNKVCYPKGIAACLGALAGVALKQDHQIRAARLLGAAESLLNAVAGKLFPTDQADYD
                     RAATALRSQMTEQTFAAAWAEGGAMTLEQAIDYALKTETD"
     gene            complement(16177..16350)
                     /locus_tag="AC162G_02325"
     CDS             complement(16177..16350)
                     /locus_tag="AC162G_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074681.1"
                     /translation="MTEKTAAWLTLNLPQIVVTRSPVEPDRLVEGIAFVSVETYPIEL
                     IFDGTQWSIHESP"
     gene            complement(16371..>16658)
                     /locus_tag="AC162G_02330"
     CDS             complement(16371..>16658)
                     /locus_tag="AC162G_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074682.1"
                     /translation="DVVVVTSDDAKSMNGLLAVVDRYRVGAILSVDIGNTRAGREWLD
                     MIAAKQIDVIEAGLDVGIEDGVSLSRAESGWVRIELGTIRSASASPDRDHA"
BASE COUNT         3482 a         4921 c         4807 g         3448 t
ORIGIN      
        1 cgagttcatt ttgcgccttg accgtgccgg aatacttgcc agtccgtgta atccgtgtga
       61 cagatcatta agtacagttt cataaatttt tagggaaagc tggatgggtg tacactcaag
      121 gatgtgatcc ataacaactt ttgtgtttgt acttagggta aatcacaacg tacctttggc
      181 aagtttagcg ttatgaacga gcaactgttg ggctatctgt ttgacgataa gccgcacatc
      241 ttggccgcgc caatgagccg gtggatcggc ttatcgccac gcttcaaagc gtttgctact
      301 acctaccgta ataagattcg caaaagaatc agagtgaccc agagcggcgc aggtatcaaa
      361 gacctggagg ttgagttaga gaccgcttat tggctgctcc aggaacaacg ctttgccgtg
      421 gcctatgagc catacagcgg cgaaaagacg cgcggacctg actttgccgt taccttcaag
      481 tccataacct ttaatgtcga agtaactcgc atcggttcct gggaaggaga gcggccattg
      541 cctgcaccag agggtgtaac gacgccctgg cttgatccac aacaggtggg cggccggctg
      601 acggataccg tctgtgacaa gctcggccaa atgcggccag ggatgataaa tgtactgatc
      661 gtaaccacgg gcagcgactt tatccgtgtt cttgaactaa atcaggcgat ggcgcggcta
      721 aaggaacgag ccgagcgcaa agaggccggc ctgttcggcc gttatggctt tgtcaatacg
      781 gccgattttt tcaagtatta tctgcgtttg agcggggtgc ttgtccgcca tgtcggtaag
      841 caagatgagg cggctgattc gatcctatgg gccaataatc aggctaaaca tcccattcct
      901 agccgcatac ggacgatcct tcaacgagga taaaggttca gaataatcat gctatactca
      961 gcgcgggcac aatttgcggt tttgttaacg ttaccatgac ggccacggtc atgcccgaat
     1021 gctgttgtcg ggcatccagt ttgcccacga ctagatcggg ggttggtact ccatgccgat
     1081 agggtagctg gattcccgcc taaagcgtgt gggaatgacc aagaggcgca gtttatgacc
     1141 gtgagcagta tactttcgtc cggggcgatt ggtctgtgcc cgacacgatc ctgaagacaa
     1201 aactctatat cccccccgcg cgctcgaagc tggtgctgcg gccgcacctg atatcccggc
     1261 tgagtgaggg cctggccggc aaattgatgc ttgtgtccgc gccggctggc tccggcaaga
     1321 ccaccctcgt cagcgaatgg atacaccgcc tcgctggaga ggatcaagct gtcgcctcag
     1381 cgtttcccgc cgttgcctgg ctctcgctcg acgaggacga caacgacgtt gcgcgcttct
     1441 ggagttacgt gatcgccgcg ctcgacacga tccggccagg tgtaggcacg gacgcattgg
     1501 cgatgttgcg ctcggcgcaa cccccgccct atcagacgat tctcacagcc ctaatcaacg
     1561 atctggacga tcggcctgac ccgttggcgc tgatcctgga tgattatcat gcgatccatg
     1621 tccagccgat tcatgatgcg ctggcctacc tgctcgatca cctgcctccg cacacgcatc
     1681 tgatcatcgc gacacgcgcc gatccgcctc tgccgctgtc gtccctgcgc gctcggcggc
     1741 agctggtcga agtgcgtgcc gacgacttgc gcttcacgcc tggcgagatc gccgcgtttc
     1801 tcaatcagat catgggcctg gggctgtcgg cggacgatct gcgagcgctg gagcagcgca
     1861 ccgaaggttg ggccgcaggg ctgcagctgg cggcgctatc cctgcaagag gaagaggatg
     1921 tcgccgggtt catccgggca tttaccggca gccatcgcta tgtattcgac tatctggcgc
     1981 aggaagtgct ggaacgacag ccggattctg tcaggacctt cctgatgcgg acgtcgatcc
     2041 tggagcgcct gagcggatcc ctgtgcgacg ccttgaccga acggacggac gggcaatcaa
     2101 cgctcgaacg gctggaacaa gccaacctgt tcatcgtgcc tctcgatcag agccgacgct
     2161 ggtatcggta ccatcctctg tttgccgatt tccttcgcgc gcagctgcag caggctatcg
     2221 gcgcacatgg catactggcg ctccatctcc aggccagcga gtggcacgaa caaaatggct
     2281 cgattcgaga tgccgtcagc cacgccctgg ccgcggacga tcacacgcgc gcggcgcgcc
     2341 tgatcgagga ttatgcgccc gcgatgtttc ttcagagcga actgaccacg ttgaccggct
     2401 ggattcacag attgccggtc gaagatgtct atgcccgtcc gcgactgtgt atggccaacg
     2461 cctgggcgct gctggccacc ggacatggtg aagaagccga acggtggctg caggccatcg
     2521 agcgcacatt gggcgccagc gccgaaggtc ttgcagacga agattcaggc agactatcgc
     2581 cagatgtcct cggcgcgctc gtcgaggtca cggcattgcg aggcgtcctg gcagtggggc
     2641 gcttcgatgt cccccgcacc ctcgaactta gccggcgtgt cttgcgccat ctcggcgaca
     2701 gcgaactgcg cggtcttttc aacgatccgc cacagatgcg gcctgcggcg ctgttcaact
     2761 taggactggc gcaagaactc ggcggcgaag tcggcacggc ggccgggaca ttccgcgaga
     2821 ccgccgcggc gagtcttgag ctgaagaatc agcacctcta tccgctggcg ctgggtcatc
     2881 tgggccagct acaggtcgtg caaggtcagc ttcaccaggc acaacttacg tatcggtcag
     2941 ccttgcagga aacccaggaa ttcgcggggt cgccttcgcc gctgtcggcg atcgcgcacg
     3001 caggtctggg gaatgtgcac tatgaatgga acgaccgcga cgcggcgctg tctcacttgc
     3061 tcaaagggat agaattggcg aagccgtgga tcaactggga aagtctgatt ccagcctatc
     3121 tcggcttggc cagattgaag cgatctcggg gcgacctgga cggcgcgctc accgtgctcg
     3181 acgaattcat agcgatgtgc cggcaatcga acgcggccat gacgtcggcc gcccaagcct
     3241 accaggccgt gctgtgggcg gcgcgaggcg actctgcatt agcgactcaa tggctgcgtg
     3301 aggcaggcat agacccggcc ggcgatctgg cttacggcgt agagttcgag gccatagcct
     3361 cggcgcaagt cttgatcgca ctgggccgcg ccgccgaggc cgcgcggctg ctggaacggc
     3421 tgcggcagag cgctgaagcg ggccacagga cgggacgcgt catcgagata tcggtgtggc
     3481 agtctgtggc gcagcatgcg caggcaaaag cgccggaagc gctgtccgtt ctggaaaggg
     3541 ctgtcactct ggctgcgccg gaaggatact gccgcacgtt cgtcgatgcc ggcgcgtcac
     3601 ttgccgaatt gctggccaaa ctgctcacga cgcggcgttt ggaccttgac gagacgagct
     3661 cgacatatat tcgtcggttg ctggacgcca tgagagtcga tcgtgaaggc aagggagcgg
     3721 cagccgtgcg ggcgcagcca tccaaatccc cgccggccgg aatggccgaa gcgctcagcg
     3781 atcgagaaat ggaagtgtta cggcgtttgg ccgatgggtt gaccaatgca gagatcggcc
     3841 aggcgctggt tctgtcggtg aataccgtca aaacccatgt cggaaacatc tacggcaagt
     3901 taggcgctcg caatcgcgcg caggccattg cacgcgctcg cgaactggat ttgttgtaga
     3961 gcctcgtggc agcggcatcc ccatgccgta gtcttcctct cgtcacccgt tcgggtgacc
     4021 cttcgcctac gcaaatcacc cctcgaatcg tcctccaggc tgatgacggc gcccgtctcc
     4081 tttgctatgc tggtcgcgac cactgcatag gaggtgcgcg acatgaatga gactttagtg
     4141 accgtgcagg acttccggaa agtctacggc gatattgtcg ccgtcgacgg catcagtttc
     4201 caggcgcggc gcggcgagat cttcggcctg ctgggcccca acggcgccgg caagaccagc
     4261 acgctggaat gcctggaagg tctccggcgc gcggatggcg gcaggctcga catcatgggt
     4321 gtggacccgg gccgcgacac gcgaaagctg caaaacctca tcggcgtgca gttgcagacg
     4381 tccggattgc cggcggacat gagcgtggtc gaggccatga aattcttctg cgcctatcac
     4441 ggcgcggcgc cgcgcttcga cctgctggag cggctggggc ttggcgagaa gctgcgcagc
     4501 acgtaccacg aactgtcgac cggccagcag cgccgtctgg cgctggccct ggcagtggcg
     4561 caccagcccc cgatcgtgat cctggacgaa ccgacggcgg gtctggacgt agcttcccgc
     4621 gtcgagcttc acgctgtgat gcaggagctg cgcgactcgg gcagcagcat cctgctggcc
     4681 acgcacgaca tggccgaagc ggaaaagatg acggatcgcg tggccattct gttgaaaggt
     4741 cgcatcgtcg ctacaggctc gccgcgcgag ctgaccgcca ccggagccgg cttcaccaaa
     4801 gtgtccgtcc gcagcgaaaa cgccgcgctg tcgcgcaata atgtctcctt cccggctgtg
     4861 gggcagcacg tgttcaaaga cgagtatgcc atctacttca gcaccgatcc ggctccgacc
     4921 gtgtcggcga ttctggagta tctcgcggcc cacgatgaca aattgatcga cctgcgcgta
     4981 gagcggcctt cgctcgaaga gcgtttcctt gagattaccc acgatggggg tgcaagatga
     5041 ctgcctttgt caaccacttt gcgttcgaat tcaaaacggg tttgcgcaac ccgacccaga
     5101 tgctgatgaa ttatctgttt ccgctggctt tctatgcctt gatgggtttg gtgatgacgc
     5161 agatcaatcc cggcttcaaa gagacgctgc tcccggctat ggtcatcttc gccgtgctgg
     5221 tcagcacgct gctcggcatg cccggacctc tggtcgaatc gcgcgaagca ggtattttcc
     5281 gcagcttcaa gatcaacggc gtgcccgcgc tttcgatact gtcgattccc gccttcacga
     5341 cgatttttca cgcgctgatc gtctccgtcc tcatcgcgct gactgcgtca tccctgttcg
     5401 gcggcgcagc gccggtggac tggggtagct tcgtcctggt cacgctcgtg acggcttttt
     5461 cctgcggcgg attgggcgcg ctcatcggcg tcatcgccag cagcgcccgc tcgaccgtgc
     5521 tgtggtcgca actgattttc ctgccgtcca tgctcatcgg cggcctgatg gtgcccatca
     5581 gcctgctccc ggaatcgata cgcccggtgt cggcgctgtt gccttcaaca cacgcgatgc
     5641 aggccttcct gggcctggct tatcggcaag agacgctcgt cgacccgctg gcgtcgcttc
     5701 tcatcctggc ggtgagcggt gtggtcgcct ttggcctggc tatctacctg tttaactggg
     5761 atagccggaa tgattcacgg cgcggccatc cactgatggc tttgctggct gtgctgccct
     5821 acctcgtagg tatcttcttt ctgaaatgaa tcgcgagggg ccaagcctgt ctcgcgcagc
     5881 ctcttcattc ggcaacgggc agatatcctc cgatctgccc gttgccgcct agatgaacgt
     5941 gtcctctccc cacgcgccag acgtatcccg cgcccagttt cgggcacgcc tcaacagggt
     6001 gttgagtcac agattggcgc tgctgatcgc gccgcgcgga tcgggcaaaa caacggcact
     6061 tcgcgactgg cgccattcgt gtggctgtct aacggcgtgg gtgtctctga gcgaacatca
     6121 caacgtgcca gagcgtttca tccgcgattt ggtcgagaca ctgtcgcacg ctgcgccgac
     6181 tttgcacgcg cgttcgatct gggagtcgga tggcacgctt acgtcactca aggaagcgat
     6241 ggctcgcctg atcaacgctc tggcggacgt gtccgatact ctcatcttaa tcctggacga
     6301 ctatgatgtc attcgggctg cgccgatcca tgaggccatg gcgatgctac tcgattacat
     6361 gccgccggcc ctgcacgtca tcatcgcctc aggcgaagac cctccacttc cgctcgcgcg
     6421 cctgcgtgtc cgcatgcagt tggtttcgct gggcatagag gatttgcaga tgacgtgacc
     6481 ggtttatcgg tgggtggcgc tctcgcctga cgatgaacgc gaggtctggg ccacctttca
     6541 gaaatacgac gacaaggact ggtcgtacgt cgactgcagc atctgggtga tgtcgcaacg
     6601 actcagagtt ccgcaagtct tcgcattcga cagccacttc aagcagatgc ctgggatggt
     6661 gtgcctgccc aaataggact gccgacagac caccagcacc tgttcacggc gcttcgtgaa
     6721 cgctccactg cgccccgtcg aagatcaact cgatagatcc actttccaac aataggtgtc
     6781 gtctccttcg tatcgagcgg ctgacctggc tggtcgtgac atgtgctgca ggaggcaacc
     6841 gcattactcc ttgatcacca gcggtagata aacagacgct tggcccgtca cattttgggc
     6901 aacgaccgta atgagctgtg ggctgtttga ggctccagcg ccagtgatca caatgtgggc
     6961 tacgcgagtg ggaccgccag gcaatgcccg tgcgtcaaca ttgaccgtta cgtcggatgg
     7021 agctgtaccc gaagccgaac tgagtgtggc ccaggttgcg tcgctggtcg ctgtccaatt
     7081 gatcgatcct gtgccgagat tgcgaatgct caaagtatgc ggaccgggat taggcccgcc
     7141 gggctgcacg aggaacaaca gcgagtccgg tgcaacactc aacatcggat tgggctgggg
     7201 cacatcggct gcgatgttga tgacaacggt cgctccgccg gtaagtccgt tggaatcagt
     7261 ggcagttaag gtgatggcat gggtgcccac tgataagagt gacgtgtgga gcagatgtcc
     7321 gatgcccaaa ggcccatcca ggcttgaact ccaactcaag taagcgtcgc ccagagttcc
     7381 atcttcaaag tcctgaccat aaccttccaa ggccacagtt tggccatagg catatacgct
     7441 gccatgagcc ggattatcga ttgctacgag tggcgcttta ccggccacac taaacgagtc
     7501 actatcggcg gaaccggtca acacgccatc gttcgccaca acgcggaagt aacctgtgct
     7561 ggctccgctc gtgccttcca actgaccaga atcaaccgcg attgtggtcg atgcgatacc
     7621 tgaagccaac gcgcgccatg tggtccgacc gttaaagctg taaagcagcg tgtagcggag
     7681 tgcatcccca tcgaggtctg cgccgtgcca tgtgagtgtt accggctggc tcgccgccag
     7741 gctcggcccg ccgctgcggg cggtgatagt cacagtcggc gcattggcac tgacgtttac
     7801 ggatgcgatc tcgcggttga cgacgtcgga aaagatcgcc acgcgtcgcg tgccgggcat
     7861 gaagttaacc gtctgcgcaa tcacacccgt ctttgccgtc aggttagtgt cttccgaatt
     7921 ggcgagcggg gtgaaggcat aatcggccaa ttgcacatgg ctggcattca acagacgaat
     7981 gtgatatggc ccggcgatca agggtggaat ttgactcacc tgcgattgtc gagagaggta
     8041 cggcaacgcg gcaacctgat ccgtaaagtt gatgacgcca tacacggcca gaaaatcgcc
     8101 cgtggtgtaa ggctggcttg atcggatatt gagagcagcg atgttccgat cgctatggcc
     8161 cgccgcaagc ggccagccga tctgcagata atcgcggatg ccggtgtaag tgacatcgga
     8221 gatccattcg ttatcgcagt aggacatgat gtcggtccag gtgtccccgg gcattacgcg
     8281 tatcggtaaa ttcgccacac caacaccgac atcaaaccca aagaagcgtg aagtattgcc
     8341 gtcgggtccg ccgataatac cattcgggta agggtaagac tgataggggc catcagtatt
     8401 acaggcatcc gtatggaacc gccacagcgt gtggccgatc tcgtgcgaac cataccaatc
     8461 gccatagctg ccgtcgaagt cccagcccca cgaagaggga cccgtcgggc cggctgcagt
     8521 cccgatcggg aatccgaacc ccaggccgcg catgaagccg ctcatattgc cgcgatcgta
     8581 gaccatgcca tagtacaccg cagcgaggtt agcaggctcc acgatcgagc gtaatgctag
     8641 cagttcggca atcacgatca cggcgttggg ctgatcgtca atggtgatga acggaaattg
     8701 gttcgtgaat ccgacgaacg gaccgaaatg tatcaaggta cgccgcagga cgctcagcga
     8761 actaatcggg tacatccgcc ggagctgcga ctctagcatg tcctggtcga acgtcggcgc
     8821 agagacgagc gcaccattgc tttggaaata ttcgtaattg acatcgcgca ggatcagcgg
     8881 attagtcgct cgaaaagtca ggtcgccggt cgtgactgtg ttgttatcgt agttgtcttc
     8941 ccgtatgccg tggaagggat tgatctcggc ctccagcgtg atggtgccag gcacaatcca
     9001 gttggcgggc agttcaaaca ggaaactgtc attcaactgc gctcgatcgg gcgaggtgac
     9061 gacattgatc acgccaccga gattgatcgg cgtcaagaca ccgagcggaa tgctattgcg
     9121 gaaaccgagc aggcgcgccg tcacgccggt acgattggca ttgctcgcca catgcactct
     9181 ggcaaaggtg cgtttcgaag cgacgagcac cacgctgttg ttgatatcct gaacggactg
     9241 cgtgacttca atcgccgtag gtcgcatgtc agccccagcc ggcagcgggc tggtcgtcaa
     9301 gtgcccaact ccccacgtgt tcgggatgtt ccagcgcgac cctcccggcc aacccgcata
     9361 atctccagtc gcgttgacct cttgttcggc gaaggtgatg tcgatatcgc gtgcccaacc
     9421 accccccaac gtcgcgccac tgatgcgaaa ctcagcgctc cactgtggaa gctcgcctcc
     9481 cacggtcgtg cgagctatcg agtagccgcc ggggtcgggg ccgtcgaagc ccgagccgct
     9541 tccgcgatgc acgctgacta ttccgctgta agagatggta aaggccatat catccggccc
     9601 gggcgaatca ccacctgttc cagcccggtt gatgtatacg acggcattgc ggctgttttg
     9661 caaggcaatg gccgggacag tcagaccagc catgcagacg tacagatccg tggacgtatg
     9721 cttcacatag gctgtgattg cagcgagcgc gcttgagaag gaaacagagg cggcatcggc
     9781 atattcgaat ccggatgtag cagggcagtt accgtcgaca gtgggcacca cgtcactttg
     9841 tccgatcgtc acttcgccgg tcttgaggct cgcattcccc cagctagtgg gatccatctc
     9901 gctgctagag ttcggccagc ttgaggtgaa atcgaagcca gaggcatcga cgtagtgatg
     9961 gacgagcgac aatccggccg tatgtttcca ccctcccatg gtatgacggc tgatctgaaa
    10021 ctcggcatcc catctcacct gtcccagaac agtgtaggtc cggctgaccg ccgaccagcc
    10081 gccgggatca ggaccgttaa atgcgctggt agccgggttc caataggctg cacccggatt
    10141 gccgtcgggc tgaatgaaga tgacaaagtc atttccatct gctgcgccgc cgtcgtcgtc
    10201 acgatcaacg tagaccttga cgtctgatgg gccgtcaggc gaatagtacg ggttgttaaa
    10261 gcagaagtag agataatcgg cagtatgttt gtaatagact ttgaaggtgt agacagtaaa
    10321 ctcaagcggc acgacgatct ggacatagct gtagacggag tattcaatgc agcggccatc
    10381 catcgtcgat tcgccacccc cctgcaagat attgagggtc ggtccagtgc cggcatatgc
    10441 cgtgccaata tcgagtcgtg tttcacctgg cagccacaat cctaaaaccc acgtcgtcaa
    10501 caacaacaga cgcaatccag ataagagcaa gcgtgaaagt ctttgagtgt tcatggttta
    10561 ctgtcctctt cagtgatctt ggtgttctct gcatcgccgg cgcatggact ttgccacagc
    10621 ctgcctgggg atgattcagg gcttcctcaa tcgcggctcg caggatgcgg gtgatctcaa
    10681 acttgaattc atgagtcacc tgcttcacgc gcctttgttc tctggcgttt cttctttcac
    10741 cttttccgca cgggccaaca cggctttaag gtgccccaac agcgtttgcc attcccgaaa
    10801 gtaccgcact tcgccgctca tcacgtgttt cacctggccg cgccactcgg ccttgccatc
    10861 acccaagtct tcaagccaca ctcgcaccgt gaagagatgt gtccgagggc gcggcgcatc
    10921 actcaaggtc acttgcttca tttcagggca gggccacgta ctgcccgaga aacgcccgcg
    10981 cctgtggggt atcgagccgg tacagccacg ccacttcttc acaggagtac gaattaacca
    11041 cgacgttgat ggctaccagt tgctcctggc cattgacttg cacgaagtcg gggccacccg
    11101 aatcgccgaa gcacatgttg tcactgccct ggttggctag ctgcgtgacc ataatgaagg
    11161 ccggggccaa tgcgcggtac gaaagcgtgc ccgaagcccg gaatcctgtg tccgtatagc
    11221 caaagcccgg cgacggggcg ccgtgcacat cgttgctatc tatgccgtag cccaccgcag
    11281 ttaccggcgt gcctggctta agaggcagcg aatctagaag actcgcactc ggaaggcgag
    11341 taatcggacg gtcatacacg ggtgtggcga gcaggacaac gccgaggtcg tgcgtatcgc
    11401 tgttgatgtt atagacggca gggctatagt cggggtgcgt gacgaggtcg gtgatattca
    11461 ccagagaagc gctttgggtg aatggtgagt cgaaactgac gtaagcggtc tggatcaagc
    11521 ccgcattcag ccacgattcc acggcatcgg tgcaatgacc ggcggtgaga aacacctgag
    11581 gcgcgataag ggtgcctgaa caatccggga acaactcacc gctgaggagt cggacgacca
    11641 gggttcccac gccaagatgc gtcgtatcca cattgtcgag cgcgaaagcg ctatgcatgg
    11701 gtgttaacgc ctgcgggata agagccaaaa tagccagact acgcacgata cccatgggcg
    11761 attgtgttag acgattcatc ttcataagct gcaaccaagt gtatttgcgc gattccgaag
    11821 acgctgagct tgtgattcta gtgcgcgaat ctcctgaagg atccccgctc tcacacgagg
    11881 atctgcctcc tccaccagat cctgcctcaa atcatcgatc cgggtcccca ggtcgtccaa
    11941 ctgcgtacga atccaatcgc attcggcggg ttcgcctcgc tcaatccgca cggcaaagcc
    12001 agacggagct tgggccacct ccgaaatcct aacgccttct acttggtcag caaactcagc
    12061 tccgggagtc agtaccggtc ttcgtaggtg caccggtggc cacgaagact catcgatcca
    12121 gtacacgacg accccctcac ttgatatgcc ggtggacacg cctggagtgg cattctcgta
    12181 tcggtccacc cgcatgcgtg cttcgaccag gaaataccgc tgcgatcctg ttgtcgaagt
    12241 gagtttggcc gcatacacgc tgcctggccg gcgagggctg cccaacgcct gaagagtcac
    12301 agttctcgcc ggagtgcccg gcgggatgac aacgacttca ctcccatcga gccatcccaa
    12361 cttcgacttc gtgaatgagg agggatgagt tccacaggag cagtccatga catcaaatgc
    12421 tccggggctg tcggaaatgc catataggtc cccgaactgt gtgatcgcat gcagattctc
    12481 catagcccac accccgaggg gatcatccgt gtcgacgtaa catgtccccg taatggttga
    12541 agtataggtc gaaggatatt gctggtgttg cgggctgtag aagtaccccg gtgaataacc
    12601 ccaaaaggcc cacgccggac cgggagattg tgtgaagaca atgcacgcat acaaataccc
    12661 ctcgatgaga ccttgtgccg ccctgatcgc attgtccatc gtgacgccaa gatgcacgct
    12721 accgtcagga tttctggcga gcgccacatt gaaagggccg taaatatcac cggtcagtcg
    12781 tgccctgccg tacgacaccg cccgaatgaa ggccctgaga tccgcccccg tgacaggatc
    12841 gtagaaggtt ctttcgcgca cccgccgttc ccactctgct ggcgggcgag gaaaatcggg
    12901 gacatccgcg aggatgaagg ccacctctcg agagccgttc catgccatat ttctctccct
    12961 ccttaaagaa gatgccgcct aacggtatgt tgaggatcgg ttcatatcac acctcctttt
    13021 gcgatagctg gatcggatac gagtttgaag tgagcatagc acacccaccg ttatgaaagc
    13081 gttatggcgg cgttacagca aacaaaaagg gcgacttttc gcccctcatg attcccggca
    13141 atcgtgattc acttttcaat cggtctcggt cttcagcgca tagtcaatcg cctgctccag
    13201 tgtcattgct ccaccctcag cccaggccgc ggcaaaggtt tgctcagtca tttggctgcg
    13261 caaggcagtt gcagcgcggt catagtccgc ttgatccgtt gggaacagtt tgcctgcgac
    13321 ggcattcaac aggctctcgg ccgctcccaa cagccttgcc gcacggatct ggtgatcttg
    13381 cttgagcgcc actccagcca gtgctcccag gcacgcggca atacccttcg ggtagcacac
    13441 tttgttgttt agcgcgaggc tttccttgag ccattcgatg gcctgttgat agtcgcctcg
    13501 attgagcgcc atgacgccta agcggcgaat cgggtaaccc gcgctattga tgtctccaat
    13561 agctctaaaa tgagcgacgc tttcggcata cagcctgtgt gcctgttcgt catcgtgctg
    13621 cgcgcgagaa agatcaccca gataagtgag agaccagcca gtggcgtgct tgtcatccaa
    13681 ctctcgtaac aacgccaaac tttccgtcaa gaacgatcgt gcggctgtat agtcgccctg
    13741 actgaacgcg gtagctccca acgtgccaag tgacccagcc agaatcgtct tgtcccctaa
    13801 ttccctgctt actgcgacag cctcttcaag gaaaggacgc gcgcgggacc aatcaccttc
    13861 tgaccacaag cctatagcac ccgcggccca caacgccttg gcctgtgctg aagaaggcgg
    13921 cgcccctttg gtcagcgaga tggcttgcat caaccgtctc acgccttccg tataataacc
    13981 acgcgccaac cagagatacc acagcgcgcc ggtcaggcgc agtgccgcaa taagctcgcc
    14041 acgcccgagt gaccagtcga gggctgacat cagattgtca tggtcagcca tgagttggtt
    14101 gaaccacatc acttgctcgg tgtggctgaa atgaggctcg gcttcctcgg caagattcag
    14161 aaagaactca aggtgacgca gccgcagccg ctctgattcg ctcgattgca gcagcttttc
    14221 attcgcgtac tgccaggtcg gctccaataa ccgatagcgc gactggccgc cttgcctcca
    14281 atcggaaaca actacgagcg acttgtcaat gagcctggaa aggagatcga gaatctcgga
    14341 gatcgagagg ctaccttgct cctcccgatc ggcacagact gcttcgacgg tctccagggc
    14401 aaaactgccc ataaagaccg ataaccgccg gaacagagta cgctccttct ccgaaagtaa
    14461 ctcataactc caatccattg tcgcgcgcag cgtgtggtgg cgccgtggaa tcgatggact
    14521 ggtttgcgcc agcagttgaa aggcattatc cagtcgtgcg gcgatctgtg atggtgtcag
    14581 catcgtcagg cgcgcggcgg ccagttcaat ggccaacggc agtccatcaa ggcgctggca
    14641 gatttgagca atcggcgcga cattgtctgc attgagggta aaagtcggcc acgcagcgcc
    14701 agcgcgttcg acgaagaggc gaatggcatc tgaccgtgag agcgccgcag tcgagagatc
    14761 gttcacatcc ggcagtgaga gaggagaaac aggccacact ctttcaccaa tcacatccag
    14821 cctttcgcgg ctcgtcgtca gaatgcgtaa atgcgagcac gagtgcaaca acgattcgac
    14881 gattcgagca cagggggaca ccatgtattc gcagttatcg aataccagca ataattcctt
    14941 tgatcgcagc cggttcacga tcgtgtcgag cggatcacgc cccggctgct cctggatgcc
    15001 gagaattgaa gcgatggcag gtggaatcag ggcggggtca gtcagcgtag ccacatccac
    15061 aaaccatacg ccgtcggcaa attcatgcgc caaatggctg gccacttgaa tggccagtcg
    15121 cgttttaccg cagccaccgg cccccgttaa ggtgagcagg tgcgtcacgg gcgccgaaat
    15181 gccttgcggg gaaagaatgc gcttgaggtc ttccagctct tcttcgcgtc cgatgaaact
    15241 cgtcaaatac gtcggcagat tgttcatttg tcgttgtggt gacaacgcaa agcgtgcagg
    15301 cggcgcctgc acgctctttg actctgcgta gatggcttgg gtctcaggac tcggttcgac
    15361 atccagctcg cgtttcaaga ccctgacaca agtttgatag acacgcacta cgcccgtgcg
    15421 gtcgccgctt agcgcgtata agcggatcaa ccggcgataa gtttcctcac gcaagggatc
    15481 ggaccgcagc aaatgctccg tatagcgagt cgcagaccga taatcaccta gattttcgag
    15541 gtgcaggctc aaacgttcca gcactgcgca aaactcttgc ttcaagcgct cgcgctcggg
    15601 caggatccaa tcgtcatagc agcccggcaa gagttctccg cgatagaggt tgaccgcttc
    15661 ttgcagcgat tcgggtgatt gagcctgctg gatagccttc tcgaactcgt cgacgtcgag
    15721 cgtgaaagga gaatcaggca gccattgaac ggtttgcgcg tcgctgtgca gaaagctttc
    15781 cgcatcgggt agcgcctgtc gaagccgatg aatggattgg cgcaagttgt tgtgtgcctg
    15841 gacttcggtc gaatcgggcc aaaagaggaa ggcgagatgc tgtcgcgatt gaggcgcatc
    15901 gaggtgcaac aggagatagg caaggaccga cttcaagcgc gccgtatgga ccgtccgcag
    15961 caactcatcg ccgtaaacca ggctgaattc acccaataac cgaatgtgaa gcgtcggcat
    16021 agcgccgcca atctctcgca gagcaactcg ggacggactg tgacgatagc gcgataagcc
    16081 gaaggccatc ccctcatcaa ttatctctga aaaccatccc tgcgcaacta acccggtcga
    16141 tttcctcgat ccgcccctgg tgactttagt gtctgttcac ggcgactcgt gaatgctcca
    16201 ctgcgtcccg tcgaagatca actcgatggg atacgtctct acgctgacaa aagcgatccc
    16261 ttcgaccagt ctgtccggtt cgaccggcga gcgcgtgacg acaatctgcg gcagattcaa
    16321 tgtgagccac gcggctgtct tctccgtcac ttcctccagt accagcacat ctacgcgtga
    16381 tcccgatccg ggctcgccga tgccgaccga attgtgccaa gctcaatccg aacccatccg
    16441 ctttcggccc tcgacagtga cacgccgtcc tcgatcccga catccaaacc tgcctcgatc
    16501 acgtcaatct gtttcgccgc gatcatgtcc agccactcgc gcccggcgcg cgtatttccg
    16561 atgtcaaccg acagaatcgc tccgacgcga tagcgatcca ctaccgctaa caatccattc
    16621 atcgatttcg cgtcgtcgct tgtcacaacc acgacatc
//
LOCUS       DBNHXB010000062        11432 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2193192, whole genome shotgun sequence.
ACCESSION   DBNHXB010000062 DBNHXB010000000
VERSION     DBNHXB010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11432)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11432)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11432
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2193192"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="AC162G_02335"
     CDS             complement(<1..692)
                     /locus_tag="AC162G_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase domain-containing protein"
                     /protein_id="HIR5074683.1"
                     /translation="MRQTFAEVAGIDAAGPFARIRFSAPELAPALEPGRAILARSVDS
                     YLRRTWWPSAIEAGGFAVLVADPPSLALRAGDRVDVLGPIGRGFQVENGSRNLLLVAA
                     GSSVPIPDLGPLLALIDRALAGSRSVTLAYAAPSAEQAYPVSALPADIEVIRAIGADL
                     VDLLADAIAWADQVFACGPDSFNTRLAGLVESIRFPAPRGFAQALRPVELACGAGACY
                     ACWNGSGLVCVDG"
     gene            1120..2025
                     /locus_tag="AC162G_02340"
     CDS             1120..2025
                     /locus_tag="AC162G_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HIR5074684.1"
                     /translation="MTFIDQTEVGVVVSPLLPGGVRPEPLNPGIHWVIPFLESVNRFS
                     ISQQEYTMSGAFGEGAVQGDDAVEARTSDGQQVFIDATVRYSVDPVKAVELRRKWVTQ
                     DRYISSFVRPTTRNVIYNTSARYKVEEIYGVKRAELQQVIFEQLITEYAQQGLILEAF
                     QLRNITFTEDYAKSIEEKQIAEQNALRARFLVDQQKQEAERLREEARGQRDAAITKAD
                     GDAQATVLRAQAEAEALRLVSDALKANPDLLTFRYIEKLAPGINTVLLPSNAPFILDI
                     NSLLPSSAATPAPQPSPTPEATPAP"
     gene            2218..3108
                     /locus_tag="AC162G_02345"
     CDS             2218..3108
                     /locus_tag="AC162G_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR5074685.1"
                     /translation="MDYKDYYKILGVPKSASEADVKRAYRKLARDHHPDRHPGDKKAE
                     ERFKEINEAYEVLGDPDKRRKYDQLGSRYQEWQRMGGDPRGFNWGQGNVRVEDLNDLF
                     GGQFSDFFTSIFGGDTVRGRRANVRGRDVEQNVEISLEEAFHGATLTFQKNGKHIEVK
                     IPSGVKTGSRVRVAGEGGAGAGSGPKGDLYLVVNVRPHPRFKRDGDDLSLEMPIDLYT
                     AVLGGEASVQTFDGALSLKIPPETQDGRTFKLRGQGMPHLREPGGRGDLLVKVHVRIP
                     QNLSSEEKRLFSELARLRRR"
     gene            3220..4392
                     /locus_tag="AC162G_02350"
     CDS             3220..4392
                     /locus_tag="AC162G_02350"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR5074686.1"
                     /translation="MKTRLTIWVLLSLLLTACRGMAVFEAPTSVPARAVEATPIPVRP
                     TALPQSIVIEADAEELLLINLYERVNPSIVNIDVAGAVDVGEFGSGSGFVIDVEGHIV
                     TNNHVVEDARRIYVTFADGSVAEAERVGGDPASDLAVIKIKVEAALLHPVELGDSDQV
                     KVGQRVIAIGNPFGLMGTMTVGIVSGVGRTLPADTFSEALGQFSNPNIIQTDAAINPG
                     NSGGPLLDSYGRVVGVNSAIRTTGENRANSGVGFAVPVNTLKRVVPQLIETGRVRYPY
                     MGISSDYSNAFTMPELAAALDLPTAHGVLISEVLPGGPADQAGLRGGDTERDVRGVPV
                     TAGGDIIIAIDGYPVDDFDHLISYLTARTEVGQVVKITFLRGDETLEVDLTIGERP"
     gene            4472..5578
                     /locus_tag="AC162G_02355"
     CDS             4472..5578
                     /locus_tag="AC162G_02355"
                     /inference="COORDINATES: protein motif:HMM:NF037526.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baeRF10 domain-containing protein"
                     /protein_id="HIR5074687.1"
                     /translation="MLSQTDIQQLASYQGTDVLSLYINVDPTQKSTDEYRLTLRHLLK
                     SVNGHGAAEDVERIESFFDHEYDWSGRGVALFSAQRSHLWVAYSLPLPLPNHVHVGRK
                     PYLAPLMSLWDTYGSYGVALVDRLGAKMMHYQMGELVTLDGVLGEDVRPVKPGRGRSV
                     FGQRGGFDGHAARRVAEVVRRNVKESAVAAGAFFAVHRCTQVLVGGADDVVKEFIAAL
                     PAPWPERVTGTFAAAIDVADNDLRDMTYSLLADVARRREHELADAVITAAAKGSNGVA
                     RLDDTLSAAHNGRIQTLLVSEGYDAAGYRCATCGYLTAQHLETCPFCGGKFAHIPNAV
                     EAMIEQVITQNGQVKMIANHAGLKEAGVAALLRY"
     gene            5585..5884
                     /locus_tag="AC162G_02360"
     CDS             5585..5884
                     /locus_tag="AC162G_02360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5074688.1"
                     /translation="MSVRTDPEDSETAALFDFAGKIDGKRVLEIGCGDGRLTWRYADR
                     AVRVVAIDPDADEIAAALEDLPPRLRDRVEFKAVSIQEFEPAAPAFDIVILSWAL"
     gene            5903..6325
                     /locus_tag="AC162G_02365"
     CDS             5903..6325
                     /locus_tag="AC162G_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074689.1"
                     /translation="MVHALEKIYRLLKPDGILIDIHPPPEPSSIDVRVAGQTHAAGWL
                     HETDDYGDYEAADEALATIVARRLFAIERQGIFSFLTYVDTLTELRNYVTEEWENASI
                     DDVTAARIEELMSTPERDKEIILRESVRIARLAPVPKA"
     gene            6329..7966
                     /locus_tag="AC162G_02370"
     CDS             6329..7966
                     /locus_tag="AC162G_02370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR5074690.1"
                     /translation="MQREKPPSPASKSIWLGAALFALVVALAAPAIQAWRRHRRRAHR
                     PVTMPASLAAPAAFPQQTLQPLLRDEVKPGPHPWREALKIGRSQFWRILVSLVCVAVT
                     ATMGYTYALDTRVEGWQFWVWAACVAVVVFALLPSDRPRLRLDKGLLWLAPLILAALL
                     LRVTSLETIPGGLHVDEYGVASFALQHLFPASGGTHNPFITGPASHPALYHYLIRVSL
                     DVFGNTITGLRISSALAGTLAVVATYAAVSILHDRRTALFAAALMAAYHVHVHWSRIG
                     LNNIWDSVWVPAMLAAFVWGWQQRWSGGAVLAGLAMGLSQYFYPGSRIGILLLAFVIF
                     SLWRQGADRRQLIVHTGKMLVTAACVAAPLAMFALRDPTNFLLRTRQVFAWQPEAIMI
                     LTRGQPDLPAFFWHQLTRSLGAFTTYPDNAGFYAPGVPLLIGLAVPLFVIGIARSIWT
                     RRSWSVPVLWIVVTAFLGGFLLADPPSSSHYVVAIPAICWLVAMPLSWLVENGRRPWA
                     VIALLAIVATDLIFYFGAYVPGKPGDLILPFPPLPHS"
     gene            7992..9314
                     /locus_tag="AC162G_02375"
     CDS             7992..9314
                     /locus_tag="AC162G_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR5074691.1"
                     /translation="MNVIAAPISGSSPLRAENRDLSLPTGRPRRVMLLMTPKTYRARA
                     FVEAAERLGLEVVRVVDMPPELAAHWNVPLGVDFLDLEAATRAIVEFAASRPVDAIIS
                     VDDGASLLAARASAALGLPHNSPEAAEAARNKHRMRALLAQGGVRAPEFRLCSTGDDF
                     RDLAARVECPCVVKPLRLSGSRGVMRADDPDQFVIAAGRLKALLDSIDPSPRPKPFLV
                     EDFVPGFEVALEGVLDHGRLKVLALFDKPDPLDGPFFEETIYVTPSRLPQDVQAAIAQ
                     CASDAAQALGLREGPMHAELRVNAAGPWLIEVAGRSIGGMCSKTLRFGTDVSLEELIL
                     RQACGLGIESLDRERRAGGVMMIPIPEAGILRRVEGEVEARAAPGIEDVQITARLNNP
                     IVPLPEGDSYLGFIFARGESPDEVEAALREAHRRLRFVIEPTIQLLPI"
     gene            complement(9367..9681)
                     /locus_tag="AC162G_02380"
     CDS             complement(9367..9681)
                     /locus_tag="AC162G_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HIR5074692.1"
                     /translation="MARYQKTVWFVNCAKLGRELPALDRPPFPGELGQRVFENISKEG
                     WELWKRQSVLLINHYGLNMADPSAQQFLMEQMEEFLFGEGAQMPEGWMPEGQVPAGKS
                     AK"
     gene            9752..11125
                     /locus_tag="AC162G_02385"
     CDS             9752..11125
                     /locus_tag="AC162G_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_334096917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM (seleno)protein"
                     /protein_id="HIR5074693.1"
                     /translation="MNVVLISTYELGRQPFGLASPAAWLKREGARVTCLDLAVQGLDR
                     HAIACADLVAFYVPMHTATRIAASFVARVKAINPRAHVCFYGLYAPVNEGYLRGLGAE
                     TILGGEFEEGLAALYQRLAANGGRARTSHQPEPHISLARQRFLAPDRSGLPDLSKYAY
                     LNLGDGVRRTVGYTEASRGCKHSCRHCPIVPVYDGQFRVVQRDVVLADIRQQVEAGAR
                     HITFGDPDFFNGPGHALSLVQALHKEFPNVTYDVTIKIEHLLRHARLLPVLRDTGCLF
                     VTSAAESVDDEVLAILDKRHTRQDFARAVSLLRAVGLTLCPTFVPFTPWTTLEGFLDL
                     LDHVARLDLIENVSPIQYAIRLLLPAGSKLLELPTVQEFVDEFDPVALCYPWAHPDDR
                     VDRLHAGVLAAVKEGQDRELPRQAIFRKVWHLAREAAASKSARFSPWDEHYPNTPIPR
                     LSEPWYC"
     gene            complement(11183..>11432)
                     /locus_tag="AC162G_02390"
     CDS             complement(11183..>11432)
                     /locus_tag="AC162G_02390"
                     /inference="COORDINATES: protein motif:HMM:NF015038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HIR5074694.1"
                     /translation="HLFVFLYRSDVAADNNACERALRPSVIHRKVMGSFRSEWGARTY
                     AALATVLHTAKRAGDNVFQKLVSLMGTPVLHYLNPSIA"
BASE COUNT         2016 a         3653 c         3698 g         2065 t
ORIGIN      
        1 ccatccacgc agaccaatcc cgacccgttc cagcatgcgt agcacgcgcc ggcgccgcat
       61 gccagctcga cggggcggag cgcctgggcg aatccgcgcg gcgcaggaaa acgaatcgac
      121 tcgacgaggc cggcgaggcg cgtgttgaag ctgtccggcc cgcaggcgaa aacctgatcg
      181 gcccatgcga tcgcatccgc caggagatcg acgaggtcgg cgccgattgc gcggataacc
      241 tcgatgtccg ccggcagcgc ggaaacggga tacgcctgct cggccgacgg cgctgcatac
      301 gccagcgtca ccgaacgact ccccgcgagc gcgcgatcga tcagcgccag caaagggccg
      361 agatcgggaa tgggcacgct cgacccggcg gcgacgagca acaagtttcg gctcccgttc
      421 tctacctgaa aaccgcgccc gatcggaccc agcacgtcga cgcgatcgcc ggcgcgcagc
      481 gcgagagagg gcggatcggc gacgagcaca gcaaatccgc cggcctcaat tgcgctcggc
      541 caccacgtgc ggcgcaggta ggaatcgacc gatcgggcca ggatggcgcg gcccggctcg
      601 agcgcgggcg ccagctccgg cgcagaaaaa cgaatccgcg cgaaaggccc tgcggcgtcg
      661 atcccggcga cttcggcgaa ggtctgccgc atgcctggat tatcgttcgg gggcgggcga
      721 aatgcaagag gatagcggag tgggcacgcc gtagattcag cgtgtttcaa ggcaaatgcc
      781 gggcgtggga gcccggtcct ctctctccaa caagctgcta gaaatcggtt atctgtcggg
      841 tgaagtcagc ctgccgtcta ctggccgcgc gagaaagggg tataatctac tcgcaatttg
      901 aatccgcttc aaactcaaat cgataaatcc ttcggaggat gaaccatgag tatttcgccc
      961 attctcagca tcttgggttg gggggctatt ggcctcctgg tcctgtacgt gttttatgta
     1021 gtcagttctc gagcgcaagg tcgctctatg cgcatctcga tcgcggtcgt ggcgatcttg
     1081 ctcgtgttag ggatcgcgct gaacgcgctt ggcgcgggat tgaccttcat cgatcaaacc
     1141 gaagtgggtg tggtggtcag cccgctgttg ccgggcggtg tccggcccga gccgctcaac
     1201 ccgggcatcc actgggtgat tccattcctg gaaagtgtga atcgtttcag tatctcccag
     1261 caggaatata ccatgtcggg cgccttcggt gaaggcgccg ttcagggcga cgatgcggtc
     1321 gaggcccgca cgtccgatgg acagcaggtg ttcatcgacg ccaccgtccg ctactccgtc
     1381 gatccggtga aggcggttga attgcgccgc aaatgggtga ctcaagatcg ctacatttcc
     1441 agctttgtgc gcccgacgac ccgcaacgtg atctacaaca cgtctgcgcg ttacaaggtt
     1501 gaagagattt acggcgtgaa acgggccgaa ctccaacaag tgatcttcga gcagttgatt
     1561 acagaatatg cccagcaagg gctgatcttg gaggcgttcc aactgcgcaa catcaccttc
     1621 actgaagact acgctaaatc catcgaggag aaacagatcg ccgagcaaaa cgcgctccgc
     1681 gccaggttcc tggtggacca gcagaagcag gaagccgaac ggttgcgcga ggaagcgcgc
     1741 ggccagcgcg acgcagccat cacgaaggcc gacggcgacg ctcaggccac cgtcctgcgc
     1801 gcgcaggccg aagccgaagc gctccggctg gtcagcgacg cgctgaaggc caatcccgac
     1861 ctgctgacgt tccgctacat cgaaaaactg gcgccgggca tcaacacggt gctgctgccg
     1921 tcgaacgcgc cgttcattct cgacatcaac agcctgctgc cgtcgtcggc cgcgacgccc
     1981 gcgccgcagc caagcccgac ccctgaagcg acgccggctc cgtagtacct tcccagtcga
     2041 attgttgctt ttccagcaag gttttgccac ggaggcactg agacacggag ggtataacta
     2101 ttagaatcgc attagagtgc cgctcagcag cttgacacgc gcgtgcgatg aacataaaat
     2161 gatctagggt caggcaggct tactggaatt gtgtgcggac gggttatcgg gctatctatg
     2221 gactacaaag actactacaa gatcctcggc gtgccgaagt cggccagcga agccgacgtc
     2281 aagcgcgcct atcgcaagct ggcgcgcgat catcaccccg accggcatcc tggcgacaag
     2341 aaagcggaag agcgcttcaa ggagatcaac gaagcttacg aagtgctggg cgatcccgac
     2401 aagcgccgca agtacgatca gctcggctcg cgctaccagg agtggcagcg catgggcggc
     2461 gatccgcgcg gcttcaactg gggccagggg aacgtccgcg tcgaagatct gaacgatctg
     2521 ttcggcggcc agttctccga cttcttcacc agcatcttcg gcggcgacac ggtgcgcggg
     2581 cggcgcgcca acgtgcgcgg gcgcgacgtc gagcaaaacg tcgagatatc gctcgaggaa
     2641 gcgtttcatg gtgcgacgct cactttccag aagaacggca agcacatcga ggtgaagatt
     2701 ccgtcgggcg tgaaaaccgg ctcgagagtg cgcgtggccg gcgaaggcgg cgccggcgcc
     2761 ggcagcgggc cgaagggcga cctgtacctc gtcgtcaacg tgcgcccgca cccccggttc
     2821 aagcgcgacg gcgacgatct gtcgctcgag atgccgatcg atctgtacac ggcggtgctg
     2881 ggcggcgagg catccgtgca gacgttcgac ggcgcgctgt ccctgaagat cccgcccgag
     2941 acgcaagacg ggcgcacctt caagctgcgc ggccagggca tgccgcacct gcgcgagcct
     3001 ggaggccgcg gcgacctgct ggtcaaggtc cacgtgcgaa ttccgcagaa cctctcgtct
     3061 gaagagaaga gattattctc ggagctggcg cggctgcgcc ggcgctagcc ggcgcccgct
     3121 catccgtccc ggagtcgctc tggtaggcat tcagatcttg ccacggagtt atctctgcgc
     3181 ctgtggcgcg ccccaacgga taaccatttg aggcccttca tgaagacacg tttaacgatt
     3241 tgggtattac tgtcgctgct cctcaccgcg tgccgaggca tggcggtgtt cgaggcgcca
     3301 acgtctgtgc cggcgcgcgc cgtcgaggcg acgcccatcc cggtgcggcc gacggctctg
     3361 ccgcagtcga tcgtcatcga ggccgatgcg gaggaactcc tcctgatcaa tctgtacgag
     3421 cgcgtgaacc cgtccatcgt gaacatcgac gtcgccggtg cagtggacgt gggcgagttc
     3481 ggatcgggat cgggtttcgt catcgacgtc gagggacaca tcgtcacgaa caatcacgtc
     3541 gtcgaggacg cccggcgcat ctacgtcacc ttcgccgacg gcagcgtcgc cgaggccgag
     3601 cgggtcggcg gcgacccggc cagtgacctt gccgtgatca agatcaaggt cgaggccgcg
     3661 ctgctccatc cggtcgagct gggcgattcg gatcaggtca aggttgggca gcgtgtgatc
     3721 gcgatcggca atcctttcgg gctgatgggc acgatgacgg tagggatcgt cagcggcgtc
     3781 gggcgcacgc ttcctgctga cacgttcagt gaagccctgg gccagttttc gaaccccaac
     3841 atcattcaga ccgatgcggc gatcaacccc ggcaattccg gcggtccgct gctcgactcg
     3901 tacggccgcg tcgtcggcgt gaacagcgcc attcgcacga cgggagaaaa ccgcgcgaac
     3961 tccggcgtgg gctttgccgt gccggtcaac acgctgaagc gcgtcgtccc acagttgatc
     4021 gagacggggc gcgtccgtta cccatatatg ggcatctcgt cggactactc caacgcgttc
     4081 accatgcccg agctggcagc ggcgctcgat cttccgacgg cgcacggcgt gctgatctcc
     4141 gaggtgctgc cgggcgggcc tgccgaccag gccggcttgc gcggcggtga cactgagcga
     4201 gacgtgcgcg gcgttccggt cacggccggc ggcgacatca tcatcgccat tgacggctac
     4261 ccggtcgacg atttcgacca cttgatatcc tatttgaccg ccaggaccga agtcggccag
     4321 gtcgtgaaaa tcacgtttct gcgcggcgac gagacgctcg aagtcgatct gacgatcggc
     4381 gagcgcccct aggccgctcg aactttacca actgaatcgg gcgctatcgc ccccacacgt
     4441 gctcgtgtga ccatcccaaa aggagatgcg tatgttgagc caaaccgata ttcagcagct
     4501 cgcgtcgtat cagggaacgg atgtcctcag cctgtacatc aacgtcgacc ctacgcagaa
     4561 gtcgaccgac gaataccgct tgacgctgcg ccacctgttg aagagcgtga acggccacgg
     4621 cgccgccgag gatgtcgagc gcatcgagtc gtttttcgac cacgagtacg actggtcggg
     4681 gcgtggcgtg gcgctgttct cggcgcagcg cagccatcta tgggtggcct actcgctgcc
     4741 cctgcccctg ccgaaccatg tgcacgtggg gcgcaagccc tatcttgccc cgttgatgtc
     4801 gctgtgggac acgtacggca gctacggtgt ggcgctggtc gaccggctcg gcgcgaagat
     4861 gatgcattac cagatgggcg agctggtgac gctagacggc gtgctgggcg aagacgtccg
     4921 ccctgtcaag cccgggcgcg gccggtcggt cttcggccaa cgcggcggtt tcgacggtca
     4981 cgcggcgcgc cgtgtggccg aagtcgtccg gcgcaacgtc aaggaatcgg cggtggcggc
     5041 aggggcattc ttcgccgtgc accgctgcac gcaggtcctc gtcggtggag ccgacgacgt
     5101 ggtgaaggag ttcattgccg cactgcccgc accctggcct gagcgcgtga ccggcacatt
     5161 cgccgccgcc atcgacgtcg cggacaacga cttgcgcgat atgacgtact ccctccttgc
     5221 cgatgtggcg cgtcgccgcg agcacgagct ggccgatgcg gtcatcaccg ccgccgccaa
     5281 aggctcgaac ggcgtggccc ggctcgacga caccctcagc gccgctcaca acggccgcat
     5341 ccagacgctg ctcgtgtcgg aaggctacga cgcggccgga tatcgctgcg cgacgtgcgg
     5401 ttacctcacc gcgcagcatt tggaaacctg cccgttctgt ggtgggaagt tcgcgcacat
     5461 ccccaacgcc gtggaggcca tgatcgagca ggtcatcacg cagaacggcc aggtcaagat
     5521 gattgcgaat cacgccgggc tgaaggaagc cggcgtggcg gcgctgctgc gatactgacc
     5581 cgatatgtct gtgcgcaccg atcccgaaga tagcgaaact gccgccctgt tcgatttcgc
     5641 aggcaagatc gacggcaagc gggtattaga gattggctgc ggcgacgggc gcctgacgtg
     5701 gcgctacgcc gatcgcgccg tgcgcgtcgt cgccatcgat cccgacgccg acgagatcgc
     5761 cgctgcgctg gaagacctgc cgccgcgact ccgcgatcgg gtcgagttca aggcggtgag
     5821 catccaggag tttgagccgg ctgcgcccgc gttcgacatc gtgattctat cgtgggcgct
     5881 ctgatgaatc gagcgcgcgg gcatggtgca tgcccttgag aaaatctacc gcctgctgaa
     5941 gcccgacggc attctcatcg acatccatcc tccacccgag ccgtcttcga tcgacgtgcg
     6001 cgtcgccggc caaacacacg cggctggctg gctgcacgag accgacgact acggcgacta
     6061 cgaggctgcc gacgaagcgc tggcgacgat tgtcgcgcgc cggctgttcg cgatcgagcg
     6121 ccagggcatt ttctcgttcc tgacgtatgt ggacacgctg accgagctgc gcaactacgt
     6181 gaccgaggag tgggagaatg ccagcatcga cgacgtcacc gcggcgcgga tcgaggaatt
     6241 gatgagcacg ccggagcgcg acaaggaaat catcttgcgc gaatcggtgc gcatcgcgcg
     6301 gctggcgcca gtcccgaagg cttgagatat gcaaagggag aagcctccct cgccggcgag
     6361 caagtcgatc tggcttggcg cggcgctctt cgcgctggtc gtcgcattgg cagcgcccgc
     6421 gatccaggcg tggcgccggc atcggcgccg agcacacaga ccggtgacaa tgcccgcgtc
     6481 cctggcagct ccagccgcat tcccgcagca aacgctgcag cccctactcc gagacgaggt
     6541 caagcccggt cctcacccct ggcgcgaagc tctgaagata ggtcgctccc agttctggcg
     6601 gattctggtg agcctggtgt gtgtggcggt gaccgccaca atgggataca cgtatgccct
     6661 ggacacccgg gtcgaaggct ggcagttttg ggtgtgggcg gcctgcgtgg ctgtcgtcgt
     6721 cttcgccctg ctgccgagcg atcggcccag gctgcggctg gacaagggcc tgctctggtt
     6781 ggcgcccctg atactggccg cgctgctgct gcgtgtcaca tccctggaga ccataccggg
     6841 gggactgcac gtcgacgagt acggcgtggc cagcttcgcg ctgcagcacc tgtttccggc
     6901 ctcgggtggc acgcacaatc cgttcatcac aggaccggcc tctcatccgg cgctgtatca
     6961 ttacttgatc cgggtttcgc tcgacgtgtt cggaaacacc atcacaggct tgcgcatctc
     7021 cagcgcgctg gccggcacac tggctgtggt ggcaacctac gccgccgtgt cgatcttgca
     7081 cgatcggcgc acggccctgt tcgctgcggc gctcatggca gcctatcacg ttcacgttca
     7141 ctggtcgcgc attggcctca acaacatctg ggacagcgtc tgggtgccgg cgatgctcgc
     7201 cgcattcgtg tggggatggc agcagcgctg gagcggaggc gctgtcctgg cgggcctggc
     7261 aatggggctt tcgcaatact tctaccccgg cagccggatt ggaatactct tgctcgcctt
     7321 cgttatcttc tccttgtggc ggcagggcgc cgatcggcga cagcttatcg tgcacaccgg
     7381 caagatgttg gtgaccgccg cctgcgtcgc agcgcctctc gccatgttcg cgctgcgcga
     7441 cccgactaat ttccttttgc ggacgcgcca agtcttcgcc tggcagcccg aagccatcat
     7501 gattctcacg cgcggacagc ccgacttgcc ggcgttcttc tggcatcagc ttacgcgctc
     7561 gcttggagcg ttcacgacgt acccggacaa cgccgggttt tacgcgccgg gtgtgccgct
     7621 gctgatcggg ctggctgtgc ctctcttcgt gataggcatc gcgcggtcga tctggacacg
     7681 acgctcgtgg agcgtgccag tgctgtggat cgtggtaact gccttcctcg ggggtttcct
     7741 gctggccgac cccccgtcca gctcgcatta cgtcgtggcg ataccggcga tctgctggct
     7801 ggtcgccatg ccgctgagct ggctggtcga aaacggccgg cggccgtggg ccgtcatcgc
     7861 cctgctggcg attgtggcga cggacctcat cttctatttc ggcgcctacg tgccgggtaa
     7921 gccgggcgac ctgatcctgc ccttcccgcc gttgccgcat tcgtgagcgc aagcggtata
     7981 attcaggcgc aatgaacgtc atcgccgctc ctatctccgg ttcttccccg cttcgcgccg
     8041 agaaccgcga tctctcactc ccgacaggac ggcccaggcg tgtcatgctg ctgatgacgc
     8101 cgaagacgta tcgagcgcgg gcgttcgtcg aagcagcgga gcggctgggt ctcgaagtcg
     8161 tcagagtcgt ggacatgccg ccggagctgg ccgcgcattg gaatgtcccg ctcggcgtcg
     8221 actttctcga tctcgaagcg gcgacgcgcg cgatcgtcga gttcgccgcg agccggcccg
     8281 tggacgcgat catctcggta gacgacggcg cttcgctgct ggcggcgcgg gcgagcgcgg
     8341 cgctcggcct accgcacaac tcgccggaag ccgccgaggc ggcgaggaac aagcatcgca
     8401 tgcgcgcgct attggcgcaa ggcggcgtgc gcgcgccgga attccggttg tgctctacag
     8461 gcgacgattt ccgcgatctg gctgcgcgcg tcgagtgccc gtgcgtcgtc aagccgctgc
     8521 ggttatctgg cagccgtggc gtgatgcgcg ccgatgaccc ggatcagttc gtgatcgctg
     8581 ccggacggct gaaggcgctg ctggactcca tcgatccatc acccaggccc aagccgttcc
     8641 tggtggaaga tttcgtccca ggtttcgagg tggcgctgga aggcgtgctc gaccacgggc
     8701 gattgaaagt gctggcgctg ttcgacaagc ccgatccgct cgacgggccg ttcttcgagg
     8761 agacgatcta cgtcacgccg tcgcgcctgc cccaggacgt acaggcggca atcgcgcaat
     8821 gcgcatcgga tgcggcgcag gcgctcggcc tgcgcgaggg tccgatgcac gccgagctgc
     8881 gcgtcaacgc agccgggccg tggctcatcg aagtggcggg ccgatcgatc ggcgggatgt
     8941 gctcgaagac gctgcgcttc gggacggacg tctcgttaga ggaactgatc ttgcggcagg
     9001 cgtgcggcct gggcatcgaa tcgctcgatc gggagcggcg agcgggcggc gtgatgatga
     9061 taccgattcc agaagcgggc atcctccggc gcgtcgaagg ggaggtcgaa gcgcgggcgg
     9121 cgcccggcat cgaggacgtt cagatcacgg ctcgcctgaa caatccgatc gtgcccttgc
     9181 ctgaggggga tagttatctg gggttcattt tcgcgcgcgg cgagtcgccg gacgaagtcg
     9241 aagccgcgct gcgcgaggcg catcggcggc ttcgattcgt gattgagccg acgattcaac
     9301 tgctgccgat ctgacgaacg accaaaggcg aagaacgagc gacgagtttc gtcgttggtc
     9361 tttcgtctac ttggcgctct tgccggctgg cacctgccct tccggcatcc agccctcagg
     9421 catctgcgcg ccttcgccga agaggaactc ttccatttgc tccatcagga actgctgcgc
     9481 gctgggatcg gccatgttca acccgtagtg gttgatgagc agcaccgact gccgtttcca
     9541 caactcccag ccctctttcg atatgttctc gaagacgcgc tggcccagct cgccgggaaa
     9601 cggcgggcga tccagcgccg gcagctcgcg ccccagcttc gcgcagttga caaaccaaac
     9661 tgttttttga tatcgagcca tgaacgcgcc tcgtcgcgca cggaagttgg atagactata
     9721 tccgctttcg agcgtgtgtc aaggtggcat tatgaacgtc gtcctgattt ccacatatga
     9781 actcggtcgc cagccgtttg gattggcgtc gcctgcggcc tggctgaaac gcgaaggtgc
     9841 gcgcgtcacg tgcctcgatc tggccgtgca gggcttggat cggcacgcga tcgcgtgcgc
     9901 cgatttggtc gccttctacg tgccgatgca caccgcgacg cgcatcgccg cgtcgttcgt
     9961 cgcgcgggtg aaggcaatca acccacgcgc gcacgtctgc ttctacggcc tgtacgcgcc
    10021 ggtcaacgaa ggctacctgc ggggactcgg cgcagagacg atcctgggtg gggagtttga
    10081 agaaggactt gccgcgctgt atcaaagatt ggctgctaac ggcggcaggg cgcgaacgag
    10141 tcaccagccg gaaccgcaca tttcactcgc ccgccagcgc ttcctggcgc ctgaccgcag
    10201 cgggctgccg gacctgtcca agtatgccta tctgaacctt ggcgatggcg tccggcgcac
    10261 ggtcggctac accgaagcca gccggggttg caaacattcg tgccgccact gccccatcgt
    10321 cccggtctac gacggacagt tccgcgtcgt ccagcgcgac gtggttctgg cggacatccg
    10381 ccagcaggtc gaagcgggcg cgcgccacat cacgttcggc gaccctgact ttttcaacgg
    10441 gccgggccac gccctatcgc tcgtccaggc gctgcacaag gagtttccaa acgtgaccta
    10501 cgacgtaacg atcaagatcg agcatctctt gagacacgcc cgcctcctgc cggtcttgcg
    10561 cgacacgggc tgcctgttcg tcaccagcgc cgccgagtcg gtcgacgacg aggtgctcgc
    10621 gatcctcgac aagcggcata cgcggcagga cttcgcgcgc gccgtctcgc tgctgcgcgc
    10681 cgtcgggctg acgctctgcc cgaccttcgt gccattcacg ccgtggacga cgctcgaagg
    10741 tttcctggac ttgctcgacc acgtcgccag gctcgatctg atcgagaacg tgtcgcccat
    10801 ccagtacgct atccggctgc tccttcctgc cgggtcgaag ctgctggagc tgccgacggt
    10861 tcaagaattc gtcgacgaat tcgacccggt cgcgctgtgt tatccgtggg cgcatcccga
    10921 cgatcgggtc gaccgattgc acgccggcgt gctcgccgct gtcaaggaag ggcaggaccg
    10981 ggagctgccg cgtcaggcga ttttccggaa ggtgtggcat ttggccaggg aggctgctgc
    11041 atccaagagt gcgcggtttt caccgtggga cgagcactat cccaacacac cgatcccgcg
    11101 cctcagcgaa ccgtggtact gctgagcgga gccgaccgag gagcagtttg ctcctgagct
    11161 catctaacaa cctcgtaact gctcacgcga tggacgggtt gaggtaatgc aagaccggcg
    11221 tgcccatcag gctgaccagc ttctggaaga cgttgtcgcc tgctcgcttg gccgtgtgca
    11281 gcacggtggc cagggcggcg taagtccgcg cgccccattc cgagcggaaa ctgcccatga
    11341 ctttacgatg gatcaccgac gggcgcaggg cgcgctcgca ggcgttattg tccgccgcca
    11401 cgtccgagcg gtacaggaac acgaacagat gc
//
LOCUS       DBNHXB010000063        11919 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3199655, whole genome shotgun sequence.
ACCESSION   DBNHXB010000063 DBNHXB010000000
VERSION     DBNHXB010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11919)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11919)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11919
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3199655"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2237
                     /locus_tag="AC162G_02395"
     CDS             <1..2237
                     /locus_tag="AC162G_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5074695.1"
                     /translation="IGATMGALLVVPILIAGFGLIGGLGVLLVALAVATQTLPQREVN
                     RVVIVSLFAGLLAGIIDLLSPATQLAVPGLQTFLVGLAAMLLVAYGFVIIRQFRSYAL
                     STKLIITLISITLISLGMLAVLNNATLGPAIAQQVGGILKSTATSRALIIAEALDKEM
                     DTLLTLAMNGVLVDGVETVNAAYASDPAARQDQIAQLDRQWIAAADTDPLIQSRLGND
                     MAVSLREYRDAFSENAEVFLTDQYGALAAATNRTSDYNQADEEWWQAAFSDGQGGIYI
                     GQPEFDESSASFSLRIAVPVRAPNAETIAGVLATSYRADALIGLVTSEQMERAGRIDL
                     LLPNGHILESQGTKALDPNVLAELRAAATQDYVEMEYDGTLSLVSQAPVAHLESEEEA
                     TPIENLGWSLIIHQDRSAVLATFDNQTRATLLMALVIAGLSAGVAIGLAYLLAGPITR
                     LTAVADQVRAGDLSARARVESGDEVGTLAATFNSMTTQLKETLEGLEQRVAERTKALA
                     TSTEVSRRLSTILDQKKLVSEVVEQVQSAFNYYHAHIYLFDEARENLVMVGGTGEAGA
                     TMLARGHRIPHGKGLVGRAAETNTVVLVRDTSTDPGWLPNPLLPETESEVAVPIAIGE
                     QVLGVLDVQHNVVGGLKQEDADLLQSIANQVAIAVRNAQSFAQAQQQAERESLVNAIS
                     QKIQSATTVDHALQVAVRELGRALSARRTSVQLSSAARPGNGQQLATEPQSNVPQGGT
                     PTGS"
     gene            2278..5223
                     /locus_tag="AC162G_02400"
     CDS             2278..5223
                     /locus_tag="AC162G_02400"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR5074696.1"
                     /translation="MSEDELTHQLEQLFSDPAPADTPQAAEAAALQAQVAELEAAMAA
                     VQAQAGQEHERQEAVTIEETAAPDQGLPIARREPPADTADHGAEGQGQRLNLLRYFSV
                     VSSIAFIVAIVLLGVFYRQTAVSDLIKLEESKNAALTQAFANSLWPQFAPFVTSATGL
                     SSDELRTHPETAKLRQAILAQVKGLAVVKVKVYDLEGLTVFSTEESQIGQDGSTNAGF
                     LSARSGGVASLLTHRDTFNAFEGEIEDRDVFSSYSPVRRGGSTGPIEGVFEIYSDVTP
                     LLQEIEQTQRNVVIGVTVILASLYAVLIFVVRYAQTVIQRHRVERGWVEEKLRTLNTE
                     LEARMSARTRDLALATEVSQRLSAMRGLDILLAEAVKLIQERFDLYYTQIYLADPAER
                     TLRLRAGTGSVGEELIRRGHRLPIGPGSINGTAAYEKRALIVADTAQSAIFLPNPLLP
                     DTRSEMAVPLIAGERVVGVLDLQSAEPGALTAHNLMAFQALAGQLAIAIENASLFAEA
                     AQTRAEVEAQAQHLTRVGWQDFLNATDRRERLGYTYDLTNVTPLNEALPQEVPPSGQA
                     LAVPIVVAGEEVGGLRLESDSGRTWTVDDAELVMSVAGRVARQVENLRLLAQAEHYRA
                     EAEAATRRLTREGWEGFLASRAAPETGYVYDLNQVAPLSVEPAGSSSPFIHPLQVRGG
                     TIGELAIDGPEGLGDEAVDLVAKVAERLSAQVETLRLTEQVQAALAEQAATVTRLREL
                     DHLKSSFLANMSHELRTPLNSILGFTDVLLEGLDGPLTDTMQNDLRLIGRNGQHLLSL
                     INDVLDMAKIEAGKMNLSVERFNLGEVLEEVLDITGPLAREKSLDLRLENGSADGLEL
                     HADRIRLRQAMINLVGNAIKFTETGGIFVRAERDNGCIRISVRDTGVGVRPEQAHLIF
                     EEFGQVDTSTTRTTGGTGLGLPISKRLVEMHGGRLWVESSGVPGEGATFIIELPAADA
                     GTPPEGQAQ"
     gene            5309..7126
                     /locus_tag="AC162G_02405"
     CDS             5309..7126
                     /locus_tag="AC162G_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR5074697.1"
                     /translation="MRCESCGSAWLEACYDYAAFKREVLRGLPGRPFNLWRYREALPV
                     ADPDYPEVIPAGGTPLRQARRYAQISGGAQLYIKDERFHPTGSFKDRQAALSVAAMID
                     AGIHECVIASTGNAAVSYAAACARAGIKLWVFMTSLVPPEKLREAALFGAEVIRVSGN
                     YDQTKQIAAEFARQRGLLYERGARSLPARESMKTIAYEIVEQLGWRAPDWYVQSVSGG
                     LGPLGVFRGFQELLSIGLIDRIPAIALIQAEGCAPMARAFAAGRDTADPVVPETRIAI
                     LSTGDPGEAYTRLWRIVQRFGGTMESVGDMEAYGAMRSLARSEGMAVEPAAAVAFAGL
                     EKLIRQGRIAPHECVVVNCTGHTFPAEKHALGADPWRVDVHLSDDQTSVPREGLQAAL
                     ERLDEKTTTVLIVEDNDDDALLVQRLLEARKTYRLYRASDGGDGLARARQHRPDLIIL
                     DLMMPGLDGFHVLAELKRDERTRHIPVIVVSAKDITEAERRRLNGYIEAVYQKGSLSP
                     RAFVDQVVHVLDRRTTLAGVSQDFPEATPTDTPALRASTTLTPSPAPSLRSELALSEG
                     KGQALRSPRPEGAGSRSPEQREGEGEGSPRSRDGSEEAV"
     gene            7126..7536
                     /locus_tag="AC162G_02410"
     CDS             7126..7536
                     /locus_tag="AC162G_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5074698.1"
                     /translation="MAYSVLYIEDNPDNMLLVRRALEAHGYRFLWAATGLTGVAAAYT
                     ERPDLILLDINLPDIDGYEVARRLRSDHRNGMQYVPIIAITANALKGDAERALAAGCD
                     VYMSKPIDIRELWARVMGFLPDESPVAMGGNDNP"
     gene            7602..7970
                     /locus_tag="AC162G_02415"
     CDS             7602..7970
                     /locus_tag="AC162G_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5074699.1"
                     /translation="MPCILVVDDNDEHVELVNRILTARGYTVVAAGDAETGLQAAVDH
                     DPDLILLDLGLPDVDGQTLVGLMRRVPELTHTPIIAVTAWPQETAREMVEAYGCAGYI
                     SKPIVRITDLVDQVASHLRR"
     gene            7936..8853
                     /locus_tag="AC162G_02420"
     CDS             7936..8853
                     /locus_tag="AC162G_02420"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5074700.1"
                     /translation="MSIKLPATSVDNSCATRLQTPTQTPTQLLPASSFSIEQLTAAYN
                     QTRVDYLVPMPMNAARLAEYIHTYDVDMDRSLVAVDDGQILGLAMLGVRPGRAWVTRL
                     GVLPAKRRRGVGEALVCALLAAAERLGIDDIVLEVIQNNAPAHALFLKLGFYETGELL
                     ILRRPPAPPAGVPPARVRWLDRWEALDLLHSRSTSPAWTNETESFAHADGVLGLSLTL
                     PNGDGGWLVFQRQRLMLQRFTLMTEQGDPRAVGRALLAYLYRQYPDLDTHAENIPVDD
                     PHVKALFEAGCVESFRRIEMNRNSHITAK"
     gene            complement(8906..>11919)
                     /locus_tag="AC162G_02425"
     CDS             complement(8906..>11919)
                     /locus_tag="AC162G_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR5074701.1"
                     /translation="GDGGVQRSPDGGQTWTQVLSHTTTALAVSPLYAASQTVYAGVKE
                     TLDGPGALYRSTDGGATWQKLNTGIPATPNGGVLTITALRFAGDGTVLAGVSYGAPGD
                     DPADGAVHRSADGGQAWQLIADGLSAYQVFDVASASHQRDVEPHVSLALYAGTNGGLY
                     QFGITQFDPAEAGAWFSAGPRGGQAQALAVSPNFVDDGVAFSGEWRMGRAGDQSGLGI
                     FKSSDWGATWQASSSGIESAPFPYSSAVHDYAFSPNFATDRTVFAATWAGLFKSTDGG
                     ATWQWLPRAYFGAPGSMASVAVAPNYATGGHVMAGGGWGGLFISRDGGVNWTPNYSVT
                     SVSAIAYSGSFATSQMAFVAGANGVYKTPDGGATWTRMMTYPVSSLVLSPRFDDDHLM
                     YAGGGALYISSDEATSWITRTIAPSATLISALAISPNFNDDRTLYAGTNVGLYRSTDG
                     GLNWSVVPGYSGVPVSSLAISPGWPGHAVFLVGTARGVERIVGNAISAAQGLATLSTR
                     SIALSADETLWVVGTSDHGIYASADGGAAWTPIGLQGGPSHFSIADVSVSPDYASDRA
                     LFAARNSSVSIGGSIYRTTDSGATWSPVYSTDSVGALAISPEYAADRTVYATGNTGRV
                     LRSTDGGDTWAQVGTWPPGVSYPVLRVALPPGYPADGTVFAGGGQGFWRLPSGETTWQ
                     PAASGLISTTTVVAIAVAPNYTDSQTLLAAANWVSGMDFYGGVFRSTDGGANWQAASA
                     GLPKVEMRGVAFSPNYATDDTAYAISTSQLYRSIDGGRSWTAIGAPPDWPALSDLTVT
                     RAGHAIVTSSAGVWRYRTAARDILIDGNFEAGSGWDLATTPGTAAAAASRRVVYDGLQ
                     ALRLGIDMGGNVDATSSAQQTVTIPISATVAQLSVRWYGASSEEQMAAPEPIAPASQA
                     AGDAQYLQIVDPTSGATLETLFWTLSNAQTWQRGVFDLTPYAGQSIVLHFGVTNDGAG
                     GRTAMVVDNASLVADVLPYRVNLPLVLKSLP"
BASE COUNT         2200 a         3839 c         3878 g         2002 t
ORIGIN      
        1 tgattggcgc gacgatgggg gcgctgctgg tggtgccgat tctcatagca ggatttgggc
       61 tgatcggcgg gttgggagtg ttgttagtcg cactggcagt cgccacgcag acacttcctc
      121 agagagaagt caaccgggtg gtcatcgtca gcctgtttgc aggcctgctg gccgggatca
      181 tcgacctgtt gtcaccggca acgcaactgg cagtgcccgg cctccagacc tttctcgtcg
      241 gcctggcggc catgctgctg gtggcctatg gcttcgtgat catacgccag tttcgttcct
      301 acgcgctatc caccaagctg atcatcacct tgatatccat cacactgatc tcactgggta
      361 tgttagccgt tctgaacaat gccacccttg gcccggccat cgctcagcag gtgggcggca
      421 tcttgaaaag cacggccaca tccagggcgc tgatcatcgc tgaggcactg gacaaagaga
      481 tggataccct gctcacgttg gctatgaacg gtgtcctggt tgatggcgtg gagacggtca
      541 atgcggcgta cgcgagtgac cccgctgccc ggcaggacca aatcgcccaa ctggatcggc
      601 agtggatcgc cgccgcagat accgatccgc tgattcagtc gcgcctcggc aatgacatgg
      661 ctgttagtct gcgcgaatat cgcgacgcct tctccgaaaa cgccgaagtg ttcttgaccg
      721 atcagtacgg cgctctagcg gccgcgacga accgcacgtc ggattataac caggcagatg
      781 aggagtggtg gcaggcagcc ttcagtgacg ggcagggggg catctacatc ggccagccgg
      841 aattcgatga gagctccgca tcgttcagct tgagaatagc cgtaccggta cgcgccccca
      901 acgccgaaac cattgcgggg gtcctcgcca catcctatcg cgccgatgcg ctgataggcc
      961 tggtgacttc cgagcaaatg gaacgggccg gccgcattga tctgctgctg cccaacggcc
     1021 acatccttga aagccagggg acgaaagcgt tagacccgaa cgtcctggcc gaactgcgag
     1081 ccgccgcgac gcaggactac gtcgagatgg agtatgatgg cacgctcagt ctcgtcagcc
     1141 aggccccggt ggcacacctg gaatcagagg aagaagcaac tccgatcgag aacctgggct
     1201 ggagcctgat catccatcaa gatcgcagcg cggtgctggc tacctttgac aaccagactc
     1261 gcgccacgct gctgatggct ctggtgatcg ccggcttgag cgccggcgtg gcgatcggtc
     1321 tggcctactt gctggccggc ccgatcacgc gcctgactgc agtggccgat caggtacgcg
     1381 caggagatct gtcggcccgg gcgcgtgtgg agtccggcga cgaggtcggc acgctggcgg
     1441 cgaccttcaa cagcatgacc acgcaactga aggaaacgct ggaaggactg gagcaacgcg
     1501 tggccgagcg cacgaaggcg ctggccacca gcaccgaggt cagccgccgc ctgtccacca
     1561 tcctcgacca gaagaaactg gtgtccgagg ttgtcgagca ggtccaatcg gcgttcaact
     1621 actaccacgc gcacatttac ctgttcgacg aggcccggga gaacctggtg atggtcggcg
     1681 gcaccggcga ggccggggcg acgatgctgg cgcgcggaca caggattccg cacggcaagg
     1741 gtttggtcgg ccgcgccgcc gagaccaata ccgtcgtgct ggtgcgagac acctcgaccg
     1801 accccggctg gctgcccaac ccgctgctgc cggagaccga gtcggaggtc gccgtgccga
     1861 tcgccatcgg cgagcaggtg ctgggcgtgc tggacgtgca gcataacgtg gtgggcggct
     1921 tgaaacagga agatgccgac ctgctccaat cgatcgcgaa tcaggtggcg atcgccgtgc
     1981 gcaatgccca atcgttcgcg caggcccagc agcaggccga gcgggagtcg ctcgtcaacg
     2041 ccatcagcca gaagattcaa agcgcgacga cggtcgacca tgcgctccag gtcgccgtgc
     2101 gcgagctggg ccgggcgctg agcgcgcggc gtaccagcgt gcagttgagc agcgcagccc
     2161 ggcccgggaa cggccaacag ttagccactg agccgcaatc caatgtaccc cagggcggca
     2221 ccccaactgg ctcatgaggt cgtttcagcc cttctgtaac gtcagcggag agctcttatg
     2281 tccgaagatg agttgaccca ccagcttgag caactgtttt ctgacccggc gccggcggat
     2341 acgccacagg cggccgaggc ggcggctttg caggcgcagg tagctgaatt ggaggcggcc
     2401 atggccgctg tgcaggcgca agccggccag gaacacgaac ggcaggaggc tgtgactatc
     2461 gaggagacgg ctgcgcccga ccaaggctta ccgatagcac gacgcgaacc gccggcggat
     2521 acggctgacc acggcgctga gggtcagggt caaaggttga atcttcttcg ctatttctca
     2581 gttgtgagtt caattgcctt tattgtagcg attgttctct taggcgtatt ctatcgccag
     2641 acagcagtca gcgatctcat caagcttgag gaaagtaaga atgccgcgtt gacgcaggca
     2701 tttgcgaatt cattatggcc gcagttcgct ccttttgtga cttcggcaac ggggctgagt
     2761 agtgatgaat tgagaaccca tcctgaaaca gccaaacttc gccaagccat cctggcgcag
     2821 gtgaagggtt tagcggtggt caaagtcaaa gtctacgacc ttgaaggtct gaccgtgttc
     2881 tcaacggaag aaagtcaaat cggccaagat ggaagcacga atgccggctt tctttcagcg
     2941 cgatcgggag gcgtggcaag cctgctgacc caccgtgata cgtttaatgc ctttgaaggg
     3001 gaaatcgaag atcgtgacgt attctccagc tatagtcccg tccggcgcgg cggatcgaca
     3061 gggccaatcg aaggcgtctt tgagatttac agtgatgtca ccccattatt gcaggaaatt
     3121 gaacagactc agagaaacgt cgttatcggc gtcacggtca ttctcgcttc actctatgcc
     3181 gttctcatct ttgtcgtcag atacgcacag accgtcattc agcgccaccg cgtcgagcgc
     3241 gggtgggtgg aggaaaagct tcgaacactg aacacagaac tcgaagcgcg catgtcggct
     3301 cgcacccgcg acctggcact ggccaccgaa gtcagccagc gcctctccgc aatgcgcggc
     3361 ctggatatac tgctggccga agccgtcaag ctcattcagg agcgctttga cctgtactat
     3421 acccagattt atctggccga ccccgccgag cgtacactta ggctgagggc cggcacagga
     3481 tcggtcggcg aggagctgat tcggcgcggc catcgtctgc cgattggccc tggttcgatc
     3541 aatgggacag ccgcctacga aaaacgggcc ctcatcgtgg ccgatactgc tcaaagcgct
     3601 atcttcctgc ccaacccgct gctgcccgat acccgctcgg aaatggccgt gccgttgatc
     3661 gccggtgagc gtgtggtggg cgtgctcgac ctgcaaagtg ccgagccggg cgcgctgacg
     3721 gcccataacc ttatggcgtt ccaggccctg gccggccaac tggcgatcgc catcgaaaat
     3781 gcgagcctat tcgctgaggc cgcccagacc cgggccgagg tcgaagccca agcccagcac
     3841 ttgacgcgtg tgggctggca ggactttctc aacgcgactg atcgccgtga gcgtcttggc
     3901 tatacctatg acctgacaaa cgtgactccg ttgaacgagg cgctgccgca ggaggtgccg
     3961 ccgagcggcc aggcgctggc ggtaccgatc gtcgtcgctg gcgaagaggt tggggggctc
     4021 cgtctcgaaa gtgactcagg gcggacatgg accgtcgacg atgccgagct ggtcatgagt
     4081 gtggccgggc gggtggcccg gcaggtcgaa aacctgcgac tgctggcgca ggccgaacac
     4141 taccgcgctg aggcggaagc agccacacgc cgtctgaccc gtgagggttg ggaaggcttt
     4201 ctggcgtcgc gggccgcgcc ggagacaggt tacgtctacg acctgaacca ggtggcgccg
     4261 ctgagcgtcg agccggccgg cagtagttcg ccgttcattc atccattgca ggttcgcggc
     4321 gggaccatcg gcgaattggc gattgacggc cccgagggcc tgggtgacga agcagtcgac
     4381 ctggtggcga aggtggctga gcgcctgagc gcccaagtgg agaccctgcg cctgaccgag
     4441 caggtccagg cggcgctggc cgagcaggcg gcgaccgtga cccgcctgcg cgagctggat
     4501 cacctcaagt cgtccttcct ggcgaatatg tcccacgagc tgcgcacgcc gctcaattcg
     4561 atcctgggct tcaccgacgt cttgctcgaa ggactggacg gtccgctgac ggataccatg
     4621 cagaacgatc tgaggttgat cggccggaac gggcaacacc tgctgagcct gatcaacgac
     4681 gtgctcgaca tggccaagat cgaagccggc aagatgaacc tgagcgtcga gcgtttcaat
     4741 ctgggcgaag tgctggaaga ggtgctcgac atcaccggcc cgctggcgcg tgagaaatcg
     4801 ctcgacctgc ggctcgaaaa cggatcggcc gatggcctgg agctgcacgc cgaccgcatt
     4861 cgcctgcgcc aggctatgat taacctggtg ggcaacgcca tcaagtttac cgaaacgggc
     4921 ggcatcttcg tccgggccga acgagataac ggctgcattc ggatcagcgt gcgcgatacc
     4981 ggcgtgggcg tccggcccga acaggcccac ctcatctttg aggaattcgg ccaggtcgac
     5041 acctcgacca cgcgcacaac aggcggcacg ggtctgggcc tgcccattag caagcgcctg
     5101 gtcgagatgc acggcggccg gctgtgggtc gagagcagcg gtgtgcccgg cgaaggcgcc
     5161 acctttatca tcgagctgcc ggccgcagac gcaggcacgc cgccggaagg acaggcccaa
     5221 tagacgaggg aggacgatgg ccaagcgact cttgcaggta gagtgcgccg agtgtgggcg
     5281 ccagcagtgg caccaccctc tggatgacat gaggtgtgag tcctgcggct ccgcgtggct
     5341 ggaagcctgc tacgactacg cggcgttcaa gcgcgaagtg ctgcgcggac tgccgggccg
     5401 gcccttcaac ctatggcggt atcgcgaagc gctccccgtc gccgacccgg actatccgga
     5461 ggtcatcccg gccggaggca cgccgctgcg gcaggcgcgg cgctacgccc aaatttcagg
     5521 cggtgcgcag ttgtacatca aggacgagcg ctttcatccc accggttctt tcaaagatcg
     5581 gcaggcggcg ctttcggtgg cagccatgat cgatgccggc attcacgaat gtgtcatcgc
     5641 gtcgaccggc aacgcggcgg tgtcgtacgc cgccgcgtgc gcgagggccg gcatcaagct
     5701 gtgggttttc atgaccagcc tggtgccgcc ggagaagctg cgcgaagcgg cgctgttcgg
     5761 cgcggaagtg atccgcgtga gcggcaatta cgaccagact aagcagatcg ccgccgagtt
     5821 tgcccggcag cgtggcctgc tgtacgagcg tggggcgcgc agtctgccag cgcgggagtc
     5881 gatgaaaacc atcgcctacg aaatcgtgga gcagttgggc tggcgcgcgc cggactggta
     5941 tgtccagtcg gtgagcggcg ggttaggccc gctgggcgta tttcgcgggt ttcaagagct
     6001 gctctcgatc gggctgatcg accgcatccc ggccatcgcc ctcattcaag cggaaggctg
     6061 cgcccccatg gcgcgggcgt tcgcggccgg ccgcgacacg gccgacccgg tcgttcccga
     6121 gacgcgcatc gccattctgt cgacaggcga tccaggcgag gcgtataccc gcctgtggcg
     6181 catcgtgcag cgcttcggcg ggacaatgga gagcgtcggc gacatggagg cctacggagc
     6241 catgcgcagc ctggcccgga gcgaagggat ggccgtggaa ccggcggccg cagtggcttt
     6301 tgccgggctg gaaaagctga tccggcaggg ccgcatcgcc ccgcatgaat gcgtcgtcgt
     6361 caattgcacc ggccacacct tccccgccga gaagcacgcc ttaggcgccg atccgtggcg
     6421 cgtcgacgtc cacctcagtg acgatcagac gtccgtcccg cgcgagggct tgcaggccgc
     6481 gctcgaacgc ctggacgaaa agaccaccac cgtcctgatc gtggaggaca atgacgacga
     6541 cgccctgttg gttcaacgtc tgttggaagc gcgcaagact taccggctct atcgggcgag
     6601 cgatggaggg gatggcctgg cgcgggcgcg ccagcatcgg cctgacttga tcattctaga
     6661 cctgatgatg ccggggctgg acggtttcca cgtgctggcc gagcttaaac gcgacgagcg
     6721 gacacggcac atcccggtca tcgtcgtgag cgcgaaagac atcacggagg ccgaacgccg
     6781 acggctcaac ggatacatcg aggccgtcta ccagaaaggc tcgctctcgc ctcgcgcgtt
     6841 cgtcgatcag gtcgttcacg tgctggatcg caggacgacg ctcgccggcg tatcgcagga
     6901 ctttccggag gctactccga cagacacgcc cgccctgagg gcgtcaacaa cgctgacgcc
     6961 ctcacccgcc ccttcgctac gctcagagct tgccctgagc gaagggaagg ggcaagctct
     7021 gcgaagtccc cgaccggagg gagcgggaag ccgaagccct gagcagcgcg aaggggaagg
     7081 cgaagggtcg ccgcgctcgc gggatgggag cgaggaagcg gtctaatggc ctactctgtg
     7141 ctctacatcg aagacaaccc cgacaacatg ctgctcgtgc ggcgggcgct cgaggcgcac
     7201 ggctatcgct tcttgtgggc cgccacgggc ctgaccgggg tggccgcggc ctacacggaa
     7261 cgcccggatt tgatcctgct cgacatcaac ctgccggaca ttgacggcta cgaagtcgct
     7321 cggcgcttgc gcagcgacca ccgcaacggc atgcaatacg tgcccatcat cgccattacg
     7381 gccaacgctc tgaaaggcga cgccgagcga gcactggccg ccggctgtga cgtgtacatg
     7441 tctaaaccga tcgacattcg cgagctgtgg gcgcgagtca tgggcttcct gcctgacgaa
     7501 tcgccggtcg cgatgggcgg caacgacaat ccgtgaggag tgatcatccc cgcccgctcc
     7561 gccacaagct cgccactccc gctccacata gagagtatcg gatgccgtgt atcctggtcg
     7621 tagacgataa cgacgaacac gtcgagttgg tgaatcggat cttgaccgcc cgaggctaca
     7681 ccgtcgtggc cgccggcgac gccgaaaccg gcctgcaggc ggccgtcgat catgacccgg
     7741 acttgatcct gttagacctg ggcctgcccg acgtcgacgg gcaaacgctg gtgggcttga
     7801 tgcgccgcgt cccggagctg acccacacgc cgattattgc agtcacggcc tggccgcagg
     7861 aaaccgctcg cgaaatggtg gaggcttatg gctgcgccgg ttacatcagc aaacccatcg
     7921 ttcgaataac cgaccttgtc gatcaagttg ccagccacct ccgtcgataa ctcctgtgcg
     7981 acgcgtctcc agacgccgac tcagacgccg actcaactgc tccccgccag ctccttcagc
     8041 attgaacaat tgacggcggc ctacaaccag acgcgcgtcg attatctggt gcctatgccg
     8101 atgaacgcgg cgcgcctggc cgagtacatt cacacgtatg acgtggacat ggatcgctcg
     8161 ctggtcgccg tcgacgacgg ccagattttg ggactggcga tgttgggggt tcggcccggc
     8221 cgggcgtggg taacccggct aggggtgctg ccggcgaagc gccggcgagg cgtcggcgag
     8281 gcgctggtgt gcgcgctgct ggccgccgcc gaacgactgg ggatcgacga catcgttctg
     8341 gaagtcatcc agaacaacgc gccggctcac gccctatttc tgaagctcgg cttttacgaa
     8401 acaggcgaat tgctcatcct gcgtcgcccg cctgcgccgc cggccggcgt gccaccggcc
     8461 cgtgtccgct ggctcgatcg atgggaagcg ctcgacttgc tccacagccg gagcacatcg
     8521 ccggcttgga cgaatgaaac cgagtcattc gcccacgccg acggcgtact gggattgtcc
     8581 ctgacgctgc cgaatggcga cggcggctgg ctggtgttcc agcggcagcg attaatgctc
     8641 cagcggttca cgctgatgac cgagcaaggc gatccacgcg ccgtcggtcg cgcgctgctg
     8701 gcgtatcttt atcggcagta cccggatctc gacacccatg ccgaaaacat cccagtcgac
     8761 gatccgcacg tgaaggccct gttcgaggcg ggctgcgtcg aatcgtttcg ccgcatcgaa
     8821 atgaaccgga acagccacat cacggcgaag tagcctgcgc cgatcctggg cacagtcgaa
     8881 ggacggcgtc atcccttcgg caggctcagg gcaggctctt caggacgaga ggaagattca
     8941 cccgataggg cagcacatcg gcgacgagcg acgcgttgtc gacgaccatt gccgtccgcc
     9001 cacccgcgcc gtcgttggtc acgccaaagt gcagcacgat cgattggccg gcatacggcg
     9061 tcaggtcgaa cacgccgcgc tgccaggtct gcgcgttcga cagcgtccag aacagcgtct
     9121 ccagcgtcgc gccgctggtc ggatcgacga tctgtagata ctgcgcgtcg ccggcggcct
     9181 ggctcgcagg cgcgatcggt tcaggcgccg ccatctgctc ttcgctcgac gcgccgtacc
     9241 aacgaacgct caattgggcg accgtcgcgc tgatcggaat cgtcacggtc tgctgcgccg
     9301 acgaagtggc gtcgacgttg ccgcccatgt cgatgccgag acgcagcgct tgcaggccgt
     9361 cgtacacgac gcggcgggac gccgcagcgg ccgccgtgcc cggtgtcgtg gccaagtccc
     9421 agccgctgcc ggcttcgaag ttgccgtcga tgaggatgtc gcgcgccgcc gtcctgtagc
     9481 gccacacacc cgcactggac gtcacgatgg catgcccggc gcgggtcacc gtcaaatcgg
     9541 aaagcgcagg ccagtcggga ggcgcgccga tcgccgtcca gcttcggcca ccgtcgatcg
     9601 atcgatacag ttggctggtc gagatcgcgt aggcggtgtc atcggtggcg taattcggcg
     9661 agaaggctac gccgcgcatc tcgaccttgg gcaggccggc gctcgccgcc tgccagttcg
     9721 cgccgccgtc ggtcgagcgg aacacgccgc cgtaaaagtc catgcccgac acccaattcg
     9781 ccgccgcgag cagagtctgg ctgtccgtat agttcggcgc gacggcgatc gcgacgacgg
     9841 tggtcgtgct gatcagcccg gaggccgccg gctgccacgt cgtctcgccg gagggcaatc
     9901 gccagaagcc ctggccgcca ccggcgaaca ctgtgccgtc ggcaggatag cccggcggga
     9961 gcgcgactcg cagcacggga tacgacacac ccggcggcca cgtccccacc tgcgcccagg
    10021 tatcgccgcc gtccgtcgag cgcaggacgc ggcccgtatt gcccgtcgcg taaaccgtgc
    10081 gatcggcggc gtattcaggt gagatcgcca gcgcccccac gctgtccgtc gaatacaccg
    10141 gcgaccaggt cgcgccgctg tccgtggtgc gatagatgct tccgccaatg ctgacactgc
    10201 tgttccgcgc ggcgaagagt gcccggtcgc tggcatagtc cggcgagacc gaaacatcgg
    10261 cgatgctgaa gtgcgacggt cccccctgca agccgatcgg tgtccacgcc gcgccgccgt
    10321 cggcgctcgc atagatgccg tgatccgacg tgcccaccac ccacagtgtt tcgtccgccg
    10381 agagcgcgat cgagcgtgtc gatagcgtcg ccaacccttg cgccgcagag atggcattcc
    10441 ccacaatgcg ctcgacgccg cgcgcagtgc ccaccagaaa gacggcgtgt ccaggccagc
    10501 cgggtgagat tgccagcgac gagacgggca cgccggagta acccggcacg acactccagt
    10561 tgaggccgcc gtccgtcgat cgatacaggc cgacgttggt gccggcgtac agcgtccggt
    10621 cgtcgttgaa attcggggaa atcgccagcg cgctgatgag cgtcgcgctc ggcgcgatgg
    10681 ttcgagtgat ccagctcgtc gcctcgtcgg aggagatgta taacgcgcca ccgccggcgt
    10741 acatcaggtg gtcgtcgtca aagcgcggcg agaggaccag cgacgacacc gggtaagtca
    10801 tcatccgcgt ccaggtagcg ccgccgtccg gcgttttgta cacgccgttc gctccggcga
    10861 cgaacgccat ctgggaggtc gcgaagctgc ctgaataggc gatcgccgac acggaggtca
    10921 cactgtagtt cggcgtccag ttgacgccgc cgtcacggct gatgaacaaa ccgccccacc
    10981 cgccgccggc catgacgtga ccgccggtcg cgtagttcgg cgcgaccgcg acggaggcca
    11041 tcgatccggg tgcgccgaag taggcgcgcg gcagccactg ccacgtcgcg ccgccgtccg
    11101 tcgacttgaa cagcccggcc cacgtcgcgg cgaacacggt gcgatccgtc gcgaagttgg
    11161 gcgagaaagc gtagtcgtga accgccgagc tataggggaa cggggccgac tcgatgccgc
    11221 tcgacgacgc ctgccacgtc gcgccccaat cggaggattt gaagatgccc agccccgact
    11281 gatcgcccgc gcgccccatc cgccattcgc cgctgaaggc cacgccgtcg tcgacgaagt
    11341 tgggcgagac ggccagcgcc tgcgcctggc cgccgcgcgg gccggcgctg aaccacgcgc
    11401 cggcctcagc cggatcgaac tgcgtgatcc cgaactgata tagcccgccg ttcgttcccg
    11461 cgtacagcgc cagcgagacg tgtggctcga catccctttg atgcgaggcg gatgcgacgt
    11521 cgaacacctg gtaggcgctc agcccatcgg cgatcaactg ccaggcctgc ccgccgtccg
    11581 ccgagcgatg caccgcgcca tcggcggggt catcgcccgg cgcgccgtac gacacgccgg
    11641 ccagcaccgt gccgtcgccc gcgaagcgca gcgccgtgat cgtcagcacg ccgccatttg
    11701 gcgtcgccgg aatcccggtg ttcaacttct gccaggttgc gccgccgtcc gtcgaccgat
    11761 agagcgcgcc tggcccatcg agcgtttctt ttacacctgc gtataccgtc tggctcgcgg
    11821 cgtaaagcgg cgacacggcc agcgcggtcg tcgtgtggga caacacctgc gtccacgtct
    11881 gcccgccgtc cggcgagcgc tgcacgccgc cgtcgcctg
//
LOCUS       DBNHXB010000064         4740 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3031871, whole genome shotgun sequence.
ACCESSION   DBNHXB010000064 DBNHXB010000000
VERSION     DBNHXB010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3031871"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="AC162G_02430"
     CDS             <1..1352
                     /locus_tag="AC162G_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074702.1"
                     /translation="LERDELEQALGRLPGRVFVLHNVHAPHPIVFMSRWAMSFLRGPL
                     TKQEIRNWRLGIGDWGLEVGDRKLEVGSWRLEVGARAAEAAGQTSEIAHPQSAISAFN
                     IQHSSFSPHSPTLPPDVPQFFLPPTVAVEWAVRQWEEREGATALIAEKRLVYAPHWLG
                     IGAVRIVDRKHGVDHRHAETRLQAARAKTALIEWEKARETTVDAAALHSPPAEGALYE
                     PLPAGVATLRAVKVLAKDFEDYLYHNVAVSVPYNPALKIAGRPGDKPREFRARCEDAA
                     RAGRDAEIDRLRKSYERQIARLEEKIAREQRELERDTHEYRSRKREENWALAETAWNF
                     LRGRRPSYAVAWAMRRSGNTGRAQREIEESEDELADLREALADLQQSLEEEIAEVSQK
                     WVDLLEQVEEVRLTPRRADVSVDVFGLAWVPCWRVTLDVDGQKREVEMDAYEMEMGD"
     gene            1417..2277
                     /locus_tag="AC162G_02435"
     CDS             1417..2277
                     /locus_tag="AC162G_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR5074703.1"
                     /translation="MTRTRLLTLLALLALFLSACIVTPTPMGSALTVAFLDIGQGDSI
                     LIRSPTGMTMLIDGGNSDNDATQVILPKLREWGADRLDVMVVTHPDADHIGGLPEVLE
                     NFPVETVALTGQVHTTQIYERLLTDIRDLNVNAIQTRTGTSIPFDPDVRVEVLGPDDE
                     FVQDDNDTNNASIVIRLTYRSVSFMFTGDAEVPEEEAILASGVHLRSAILKVGHHGSR
                     SSTSELFLAVVDPQIGVISAGANNRYGHPHREVLDRLGQAGVQIYRTDQNGTITITTD
                     GLTIDVETER"
     gene            2299..2676
                     /locus_tag="AC162G_02440"
     CDS             2299..2676
                     /locus_tag="AC162G_02440"
                     /inference="COORDINATES: protein motif:HMM:NF022659.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HIR5074704.1"
                     /translation="MAEELRELRELGELRGVIDRFEGDLAAIVFDDNQRLDLPRAEMP
                     REAKAGDAVVARLGEAGAWHGKWGKKGKVALDDGQSIQWPGKAGKGEVWLSIEIDAED
                     TAARKQRVKGLLDDIFKSKPKPE"
     gene            2859..4604
                     /locus_tag="AC162G_02445"
     CDS             2859..4604
                     /locus_tag="AC162G_02445"
                     /inference="COORDINATES: protein motif:HMM:NF016202.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR5074705.1"
                     /translation="MTLVATLATALAIFQIAYGGRVLPGVRVWDVDLSGMTVERAGQA
                     IAQRFVYLDEPVLTLRAFGQSWDVAPGELGVRVDGAATARAAHRLGRGGSPLRALSSQ
                     LDMMSRGAEVAPVVSVDPNAAAAYLEARAAELNVEPRDASVSLDGLMVVVTPAQNGRA
                     LDVAATLAALSDAMRALQPAAVDMVFVDRPATITDVSAAAQQLQTLVSQPFTLYLENP
                     RPTDLHAAWDMTQEQLATLARVNRSPDGASLVVTLDRDELATDLADLATQVKVEPQNA
                     RFVFNDDTRELDVVAPSADGRELNVDATLAGIEAAIARGEHRAPIAVAETPAEFKEGV
                     TAAQLGITENVVTAQTFFAGSAAERVNNITVAASKLHGVIVKPGDVYSLGAYLGDVSL
                     DEGYAEALIIANGRTVQGVGGGVCQVSTTAFRAAFFGGYPILERWPHAYRVGWYERGF
                     GPGLDATVFVPQVDFKFKNDTPYHLLIETYVNPTAGRLTFKFYSTSDGRQVTVSDPIV
                     ENVKPHGPDVIIEDPALPVGERKQEDYAVDGADVTVKRTVMRDGVVISEDVVFTRYLP
                     WQAVYRVGTSQTTGN"
BASE COUNT          875 a         1407 c         1667 g          791 t
ORIGIN      
        1 gattggagcg cgacgagctg gagcaggcgc tgggcaggct gcccggccgc gtcttcgtgc
       61 tgcataacgt tcacgcgccg catccgatcg tgttcatgtc gcgctgggcg atgagtttcc
      121 tgcgcgggcc tctcaccaag caggaaatac gtaattggag attggggatt ggagattggg
      181 ggttggaggt tggagatcgg aagttggagg ttggaagttg gaggttggag gttggagcgc
      241 gggccgcgga agcggcgggc caaacatcgg aaatcgcaca tccacaatcc gcaatttctg
      301 cattcaacat tcaacattct tcattctccc cgcattcgcc gacgcttcct cccgacgtgc
      361 cccagttctt tcttccgccg acggtcgccg tcgagtgggc cgttcggcag tgggaggagc
      421 gcgaaggcgc gacggcgctg atcgccgaga agcgtctcgt ctacgcgccg cactggctgg
      481 ggatcggcgc cgtgcgtatc gtcgatcgca agcacggcgt cgaccatcga cacgccgaaa
      541 cacgccttca ggcagccagg gcgaagacgg ctctcatcga gtgggagaaa gcgcgcgaga
      601 cgacggtcga tgcggcggcg ctgcattcgc cgccggcgga gggcgcgctg tacgagccgc
      661 tgccggcggg cgtcgccaca ctgcgtgcgg tgaaggtgct agcgaaggac ttcgaggact
      721 acctgtacca caacgtcgcg gtgagcgtgc cgtacaatcc ggcgctgaag atcgcgggcc
      781 ggccgggcga taagccgcgc gagttccgcg cgcgctgcga agacgcggcg cgcgccgggc
      841 gcgacgcaga gatcgaccgg ctgcgcaaga gttacgagcg gcagatcgcg cgtctcgaag
      901 agaagatcgc cagagagcag cgcgagctgg agcgggacac gcacgaatat cgctcgcgca
      961 agcgtgagga gaactgggcg ctggccgaga cggcgtggaa cttcttacgc gggcggcggc
     1021 cgtcctacgc cgtggcctgg gccatgcgcc gcagcggcaa tacgggccgg gcgcagcggg
     1081 agatcgaaga gtccgaagac gagctggccg atctgcgtga ggctctggcc gacttgcagc
     1141 agtcgctgga agaggagatc gcggaggtgt cgcagaagtg ggtcgatctt ctggagcagg
     1201 tcgaggaggt tcgcctgacg ccccgccgcg ccgacgtcag cgtcgacgtt ttcggcctgg
     1261 cctgggtacc gtgctggcgc gtgacgctgg acgtggacgg gcagaagcga gaggtcgaga
     1321 tggatgcgta tgagatggag atgggagatt agaggttgga ggttggagat tggggattgg
     1381 agattagaga ttagagattg gaggttgggg aaacctgtga ccagaacccg cttgctcaca
     1441 cttcttgcgt tacttgcgct attcctttcg gcttgcatcg ttacgccaac gccgatgggc
     1501 agcgcactga ccgtcgcctt cctcgacatc ggccagggcg actcgatctt gatccgctcg
     1561 ccgaccggga tgacgatgct gatcgacggc ggcaactcgg ataacgatgc gacccaggtc
     1621 atcctcccca agctgcgcga gtggggcgcg gacaggctcg acgtgatggt cgtcacgcac
     1681 cccgacgcgg accacatcgg cgggctgccg gaggtgctcg agaacttccc ggtcgagacg
     1741 gtggcattga ccggacaggt gcatacgacg cagatatacg agcgcctgct gaccgacatc
     1801 cgcgacttga acgtcaatgc catccagaca cgcaccggca cgtccatccc attcgatccg
     1861 gacgtcaggg tcgaggtgct ggggcccgac gacgagttcg tccaggacga taacgatacg
     1921 aacaatgcct cgatcgttat ccggctcacc tacaggagtg tgagcttcat gttcaccggc
     1981 gacgccgaag tcccggagga ggaagccatt ctggcgagcg gggtgcattt gcgcagcgcg
     2041 atcctcaagg tcgggcatca cggcagtcgc agcagcacga gcgaactgtt cctcgcggtg
     2101 gtcgatccgc agatcggcgt gatcagcgcg ggcgcgaaca atcgctacgg ccacccgcac
     2161 cgggaagtgc tcgaccggct ggggcaggcc ggcgtgcaaa tttatcgtac cgaccagaac
     2221 gggacgatca cgatcacgac ggacggattg acgatcgacg tggagacgga aagatagagt
     2281 ttgttgaggg gatgacgggt ggctgaggaa ctcagggaac tcagggaact tggggagctg
     2341 aggggggtca tcgatcggtt cgagggcgac ctggccgcga ttgtgtttga cgacaaccag
     2401 cggctcgacc tgccgcgcgc cgagatgccg agggaggcca aagccggcga cgcggtcgtc
     2461 gcccggttgg gcgaggcggg cgcgtggcac ggcaagtggg ggaagaaggg caaagttgcg
     2521 ctggacgatg gccagtcgat ccaatggccg ggtaaggcgg gtaaaggtga ggtctggctg
     2581 tcgatcgaga tcgatgcgga agacactgcc gcgcgcaagc agcgcgtgaa ggggctgctg
     2641 gatgacattt tcaagagcaa gcccaagccc gagtgagtcc tcacttcctg aggcatctcg
     2701 cagagtgtgg tatcatcgcc ttgtggaaca caggcatatg ctcgcgctga gttggtaatc
     2761 ctctgcaaag ttctccggaa atgggaacaa ccacagctcg catcattaag cccccagtcc
     2821 catactcagt ctggctgctg cgaatctcat tcgcggtctt gacgctcgtc gcgacgctgg
     2881 cgacggcgct ggcgatcttc cagatagctt acggcggccg ggtgctgccc ggcgtgcgcg
     2941 tgtgggacgt cgatttatcc ggcatgaccg tcgagcgagc cgggcaagcc atcgcgcagc
     3001 gcttcgttta cctcgacgag cctgtcctca cgctgcgcgc attcgggcaa tcgtgggatg
     3061 tcgcgccggg cgagctgggc gtgcgcgtcg acggagccgc gacggcgcgt gccgcccatc
     3121 ggctggggcg cggcggctcg cccctgcgcg ccttgtcgtc gcagctcgac atgatgtcgc
     3181 ggggcgccga ggtcgcgccc gtcgtctcgg tcgatccaaa cgcggcggcg gcctacctgg
     3241 aagcgcgcgc ggcggagctg aacgtcgagc cgcgcgacgc gagcgtgtcg ctggatgggc
     3301 tgatggtcgt cgtgacgccc gcccagaatg gccgcgcact cgacgtggcg gcgacgctgg
     3361 cggcgctgtc ggacgcgatg cgcgctctcc agcccgctgc cgtggatatg gtcttcgtcg
     3421 atcgaccggc gaccatcacg gatgtatcgg ccgcggcgca gcagctccag acgctggtca
     3481 gccagccgtt cacgctgtac ctggaaaacc cgcgaccaac cgacttgcac gccgcatggg
     3541 acatgacgca ggagcagttg gcgacgctgg cgcgcgtgaa tcgctctccc gacggcgctt
     3601 cactcgtcgt cacgctcgac cgcgacgagc tggccaccga cctggccgat ctggcgacgc
     3661 aggtcaaggt cgagccgcag aacgcacgct tcgtgttcaa cgacgacacg cgcgagctgg
     3721 acgtcgtcgc acccagcgcc gacggccgcg agctgaacgt cgacgcgacg ctggccggca
     3781 tcgaggcggc gatcgcgcgc ggcgagcatc gcgcgccgat cgccgtggcg gaaacgcccg
     3841 ccgaattcaa ggaaggcgtc accgccgcgc aattgggcat caccgagaac gtcgtcacgg
     3901 cgcagacgtt cttcgccgga tcggcagccg agcgggtcaa taatatcacc gtcgccgcgt
     3961 cgaagctgca cggcgtgatc gtcaagcccg gcgacgtgta ctcgctcggc gcgtacctcg
     4021 gcgacgtgag cctcgacgaa ggttacgccg aggcgctgat catcgccaac gggcgcacgg
     4081 tgcaaggcgt cggcggcggg gtgtgccagg tgtcgacgac ggcgttccgt gcggcgttct
     4141 tcggcggcta tccgatcctc gagcgctggc cgcacgctta ccgggtcggg tggtacgaac
     4201 gaggcttcgg gccgggtctc gacgcgacgg tgttcgtccc tcaagtcgat ttcaaattca
     4261 agaacgacac gccgtatcac ctgctgatcg agacatacgt caacccgaca gccggtcgcc
     4321 tgaccttcaa gttctacagc acgagtgacg gtcgccaggt gacggtcagc gatccgatcg
     4381 tcgagaacgt caagccgcac gggccggacg tgatcatcga ggacccggcg ctgccggtcg
     4441 gcgagcggaa gcaggaggac tacgcggtcg acggcgcgga cgtgacggtg aagcggacgg
     4501 tgatgcgtga tggcgtggtg atttcagagg acgtggtgtt cacgcgctat ctgccgtggc
     4561 aggccgtgta tcgcgtgggc accagccaga cgacagggaa ctgaaaacag tgcagctggt
     4621 gattatccac aagtctgttc gtccgcagtt gcactgcgga cgccgggcga cagtgcaact
     4681 gtcgccccaa catgtgaact gaaagcagtg tcggtattga ctttcctgac gtaattctgt
//
LOCUS       DBNHXB010000065         4969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_683702, whole genome shotgun sequence.
ACCESSION   DBNHXB010000065 DBNHXB010000000
VERSION     DBNHXB010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_683702"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..3082
                     /locus_tag="AC162G_02450"
     CDS             218..3082
                     /locus_tag="AC162G_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR5074706.1"
                     /translation="MEIPGTATPWYSHTIETVARALETQLDHGLSVEEAQTRLSKVGA
                     NELREHPPEPFWKKVVDQLNSFVVILLIVASVVSALLGDWIEAVVIMAIVVLNAVLGV
                     VQESRAEQALAALKKMAAPDATVIRDGHRQRIPARELVPGDVVLLEAGNFVPADLRLA
                     ESVNLKIEEASLTGESVPVEKQASGVLAENAPIGDRRNAAYMSTLVTYGRGKGIVVAT
                     GMKTQIGLIAEMIQSFEEEPTPLQNKLNQLGKTLGLAALAICGLVFLASVLRETDLSL
                     IFDPNGGLGAYLASFRQELADTFLIAVSLAIAAVPEGLPAIVTISLALGMREMIRRHA
                     LIRKLSAVETLGSATAICSDKTGTLTLNRMVAVKLWVDHAEIDVTGHGYQPVGQFLRS
                     GREFDALGDQEISGLLWAATLANDADLEPVGETDGRISYRMVGDPTEGALLVAAAKAG
                     IWRKELEKSFPRVAEIPFDAERKRMATIHEIRDAHTDDPSPFMTGETGHVVTVKGAPD
                     LVLDCCTHLMHRKGPAPITEQERQKIRDANARMASQALRILAVAYRRLDHLPDAGDLS
                     VDSVERDLTFVGLIGMIDPARPEVRPAIDKARHAGIRTVMVTGDYPDTARAIAAEIGL
                     LRDGRTGGEGGQVLTGAQIDTMDGAALFAEVEQVDVFARVSPEHKVRIVDAFRERGHV
                     VAMTGDGVNDAPALKRADIGVAMGITGTDVSKETADMVLTDDNYVSIVGAVEQGRIIY
                     GNIRKFVFYLLSCNVAEIAIIFIAALAGWPTPLTAIQLLWLNLLTDGAPALALGMEKG
                     DPDTMDRPPRPPTEPIVNRVMVIRIAVMTVALTAVVLAAYWIGMNEHGIDLAETMAFV
                     TLALAELPIAYTTRSERYPLLRLGPFSNRWMQYAVGLSILLILAVIYVPFLNRPFNTV
                     PLTLEQWEVILPLILVPALAAEISKLFLRRRGAGRRVALV"
     gene            3185..3664
                     /locus_tag="AC162G_02455"
     CDS             3185..3664
                     /locus_tag="AC162G_02455"
                     /inference="COORDINATES: protein motif:HMM:NF015999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR5074707.1"
                     /translation="MDKTNSMRLLDARKILYHAKEYDPSREFHDAVEAAALIGAPVET
                     VYKTLVVLRESKGKSLLVMIPAHREVDLKRLAKEIGEKRLRMATQREAEQLTGLQVGG
                     ISALALLQRGFEVCLDVSAQALEMIHVSAGVRGIDLELGVKDLIAVTRAKVVRAASD"
     gene            complement(4085..>4969)
                     /locus_tag="AC162G_02460"
     CDS             complement(4085..>4969)
                     /locus_tag="AC162G_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5074708.1"
                     /translation="ERAAQAIGRGADEHLITVKGAELPAHMPQVKKSLALIYAVNPFG
                     ADHQSSEHDPMYAPDPDNNNDNLYLRRLKVLGLVDPQRGRVMNEEKVKFAYRTQLNYS
                     FLDTATLCQFVWGPAWTLYGPDEEVAMMKAVTGWDVTIDEIQKIGERRLNMLRAFNAR
                     EGAGRDKDTLPKKVLHKPLQGGRSDGRTVAPEELDFALNAYYEMAGWDKATGMPAQAK
                     LEELGLGWIGGNGAAKKAVARKAPAKKVAAKKVAAKKATARKMTARKVATKKPAARKT
                     ASQKATPKKAAKRSARKK"
BASE COUNT          883 a         1606 c         1573 g          907 t
ORIGIN      
        1 tgtcctctga cgcgagtgat attccccgct cgactcgatt cgattatata tccgattgcg
       61 caagtccgcc aagcgcaatc ccaaccgcag gcggatgtca cgcaccgggg agataatcgt
      121 ccgccaaaga tcgggcagcc agtcggtcta tgctataatc tcctcacgac aagcgtttta
      181 ccgtagcaca actttccatt tcggagccgt gtcctatatg gaaatccccg gaaccgcgac
      241 cccctggtat agccatacga tcgagactgt cgcccgcgcc ctggaaaccc aactcgatca
      301 cggcctgagc gtcgaggaag cgcagacgcg gctgagcaaa gtcggcgcca acgagctgcg
      361 tgagcacccg ccggaaccgt tctggaagaa agtcgtcgat cagcttaatt cgttcgtcgt
      421 gattttgctc atcgtcgcca gcgtcgtctc ggcgctgctg ggcgactgga tcgaagcggt
      481 cgtcatcatg gcgatcgtcg tcctcaacgc cgtgctgggc gtggtgcagg aatcgcgcgc
      541 cgagcaggcg ctggcggcgc tcaagaaaat ggccgcgccc gacgcgaccg tgatccgcga
      601 tggtcaccgc cagcgcattc ccgcccgcga gctggtcccc ggcgacgtcg tcctcctcga
      661 agccggcaac ttcgtcccgg cggacctgcg cctggccgag tctgtgaatc tcaagatcga
      721 agaagcctcg ctgaccggcg agagcgtgcc cgtcgagaaa caggcgagcg gcgtgctggc
      781 cgaaaacgcg ccgatcggcg accgcaggaa cgccgcttac atgagcacgc tggtgaccta
      841 cgggcgtggc aagggcatcg tcgtcgccac aggcatgaag acgcagatcg gcctgatcgc
      901 ggagatgatc cagtcgttcg aagaggagcc gacgccgctg cagaacaagc tcaaccagct
      961 cggcaagacg ctcggcctcg ccgcgctggc gatctgcggc ctggtcttcc tggccagcgt
     1021 gctccgcgag acggatctgt cgctcatctt cgatccgaac ggcggcctcg gcgcatacct
     1081 cgccagcttt cggcaggagc tggccgatac cttcctgatc gccgtcagcc tggccatcgc
     1141 cgccgtgccg gagggcctgc ccgccatcgt cacgatcagc ttggcgctcg gcatgcgcga
     1201 gatgattcga cgtcatgcgc tgatccgcaa gctgtcggca gtcgagacgc tgggatcggc
     1261 gacggcgatc tgctcggaca agacgggcac gctgacgctg aaccgcatgg tggccgtgaa
     1321 gctgtgggtg gaccacgccg agatcgacgt gaccggccat ggctatcaac cggtgggaca
     1381 gttcctgcgg agtgggcgcg aattcgacgc gcttggcgac caggaaatct ccggtttgct
     1441 gtgggccgcc acgctggcga acgacgccga cctcgagccg gtcggcgaaa cggacggcag
     1501 gatatcttac cgcatggtcg gcgacccgac cgagggcgcg ctcctcgtcg cggcggccaa
     1561 ggccggcatc tggcggaagg agctggaaaa atcgtttccg cgcgtcgccg agatcccgtt
     1621 cgacgccgag cgcaagcgca tggccacgat tcacgaaatc cgcgacgcgc acacggacga
     1681 tccgtcgccg ttcatgaccg gcgagaccgg ccacgtcgtc acggtgaaag gcgcgcccga
     1741 tctggtgctg gactgctgca cacatttgat gcatcgcaaa ggcccggccc cgatcacgga
     1801 gcaggagcgg cagaagatcc gggatgcgaa tgcccggatg gcttcccagg cgctgcggat
     1861 cctggccgtc gcctaccgcc gcctcgacca tctgccggat gcgggcgacc tcagcgtgga
     1921 ctccgtcgag cgcgacctga cgttcgtcgg cctgatcggc atgatcgacc cggcccggcc
     1981 cgaggtgaga ccggcgatcg acaaggcgcg ccacgcaggc atccgcacgg tgatggtcac
     2041 cggcgattat cccgacacag cccgcgcgat cgccgccgaa atcggcttgc tgcgcgacgg
     2101 aaggaccggc ggcgaaggcg gccaggtgct caccggcgcg cagatcgaca cgatggacgg
     2161 cgcggcactg ttcgccgagg tcgagcaggt cgacgtcttc gcgcgcgtca gccccgagca
     2221 caaagtccgc atcgtcgacg cgttccgcga gcgcggccac gtcgtggcta tgactggcga
     2281 cggcgtgaac gacgcgccag cgctcaagcg cgctgacatc ggcgtggcga tgggcatcac
     2341 cggcaccgac gtgtcgaagg agaccgccga catggtgctg accgacgaca actacgtgtc
     2401 gatcgtcggc gccgtcgagc agggccgcat catttacggc aatatccgca agtttgtgtt
     2461 ctacctgctg tcgtgcaacg tcgccgagat cgccatcatc ttcatcgccg cgctggccgg
     2521 ctggcctacg ccgctcaccg ccattcagtt gctatggctc aacctgctca ccgacggcgc
     2581 gccggcgctg gcattgggca tggagaaggg cgaccccgac accatggatc gacccccgcg
     2641 cccgccaaca gagccaattg tcaatcgcgt catggtgatt cgcatcgcgg tcatgaccgt
     2701 cgcgctgacc gccgtggtgc tggccgctta ttggatcggg atgaacgagc acggtatcga
     2761 tctggccgag acaatggcct tcgtcacgct ggccctggcc gagctgccga tcgcatacac
     2821 cacgcgttcc gagcgctatc cgctgctacg gctcggaccg ttcagcaatc gctggatgca
     2881 atacgcggtc ggcttgtcga tcctcctgat cctggccgtg atctacgtgc cattcctgaa
     2941 tcgccccttc aacacggtgc cgctcacgct ggaacagtgg gaagtcatcc tgcctctgat
     3001 tctcgtcccg gcactcgccg ccgaaatcag caagctcttc ctgcggcgga ggggagccgg
     3061 acggcgcgtc gcgctggtgt aacgggtcgg taacacgtct gaagcttcac cacgaaggca
     3121 cgagggcacg gagtttccga agaactttgt gtcttcgtgt cttagtggtt agtaaagcgc
     3181 caagatggac aagacgaact cgatgcggct gctcgacgcg cggaagattc tgtaccacgc
     3241 gaaggagtac gatccatcgc gcgagttcca cgacgcggtg gaagcggcgg cattgatcgg
     3301 cgcgccggtc gagacggtgt acaagacgct cgtcgtgctg cgcgaatcaa aaggcaagtc
     3361 gttattggtc atgatcccgg cgcaccgaga ggtcgatctg aaacggttgg ccaaggagat
     3421 tggagagaag agattgcgca tggcgacgca gcgcgaggcg gagcagttga ccggcctgca
     3481 ggtcggcggt atctccgcgc tggcgctgct ccagcgaggc ttcgaggtct gtctggatgt
     3541 cagcgcgcag gcgttggaga tgattcacgt cagcgcgggt gtacgtggca ttgatctcga
     3601 attgggcgtg aaggatttga ttgccgtgac gcgggcgaag gtggtgagag cagcatcaga
     3661 ctgatgccgc gtccgaccgg gttccaagtt tagaacagct tggaaactaa gccaagtcgc
     3721 agctgagcgc gaagccagcg aggatatcgc cgccgggcgc gtccacgaag ccaggaacgt
     3781 cgacgacctc atcgtccggc tcgataaggg ccgcatggcg acctgatcat tctgcgcaac
     3841 gtcggcgcgc atgatccgac gctcaagaat ccgtgacctc tggcacacac tgagccagca
     3901 agcatcatat ctgtgcatcg aagtgtgaag cccggcaata cgccgggctt cttatgcttc
     3961 ctgcacttga gaattccgat tggagtcgaa gccgcacccg cctgaaggct cgactccagc
     4021 actcggaatt cagcgtgcgc ttggtgtaag gatactcgcc gtgccggtca cctgaccgca
     4081 gacatcattt cttcctggcc gaccgcttcg cggccttctt aggagtcgcc ttctgcgagg
     4141 cagtcttcct agccgccggt ttcttcgttg cgactttcct ggcagtcatc ttcctggccg
     4201 tcgctttctt tgcggccacc ttcttcgcgg cgactttctt cgcaggcgct ttcctggcaa
     4261 cggccttctt ggccgcgccg tttccaccga tccagcccag ccccagctcc tccagcttcg
     4321 cctgtgccgg catgccggtc gctttatccc agccggccat ctcgtagtag gcgttcagcg
     4381 cgaagtcaag ctcctccggc gcgacggtgc ggccatcgct ccggccgccc tggagcggct
     4441 tgtgcagcac cttcttgggc agcgtgtcct tgtcgcggcc ggcgccttcg cgcgcgttga
     4501 aggcgcgcag catgttcagt cgccgctcgc cgatcttctg aatttcgtcg atcgtcacgt
     4561 cccagccggt gacggccttc atcatcgcca cttcttcgtc cgggccgtac agcgtccacg
     4621 ccggtcccca cacgaactgg cacagcgtcg ccgtgtcgag gaacgagtag ttcaattgcg
     4681 tgcggtaggc gaatttcacc ttctcctcgt tcatcacacg cccgcgctgc ggatcgacca
     4741 ggccgagcac cttcaggcgg cgcaggtaca ggttgtcgtt gttgttgtcg gggtcgggcg
     4801 cgtacatcgg atcgtgctcg gacgactggt ggtccgcgcc gaaggggttg acggcataga
     4861 tgagcgccag gctcttcttc acctgcggca tgtgcgcagg cagctcggcg cccttgacgg
     4921 tgatgagatg ctcgtcggcg ccacggccga tcgcctgcgc cgcgcgctc
//
LOCUS       DBNHXB010000066         4984 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_348365, whole genome shotgun sequence.
ACCESSION   DBNHXB010000066 DBNHXB010000000
VERSION     DBNHXB010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_348365"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="AC162G_02465"
     CDS             complement(<1..1313)
                     /locus_tag="AC162G_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR5074709.1"
                     /translation="MKTRKAAPRRTASVRRPKILARNGVLKAVLDNGLTVILRENHNA
                     PVATFWCWYKVGSRHERSGITGISHWVEHMLFKGTKTFPRATVDKLVSREGGVWNGFT
                     WLDFTTYFETLPAGKIDLALRIEADRMTNALFAPKEVESERTVIISERQGSENSPQFL
                     LGETLQASVFMVHGYHHETIGHLCDLQSMTRDDLYAHYRTYYAPNNCVAIGVGAFDAK
                     DMLSRIGKAFARIKPGPALPKIASVEPEQKGERRITVEGEGLTSYADVAYRAPEARHA
                     DFAPLVVLDAILTGASGLSLFGGGTTNASSRLYRALVDTELATDVSGNLVPTHDPYMY
                     AISATVRQGHTPAEVEAALDVELQKVVDQPPSQEEVDKAIRQSRAQFAYGSESVTNQG
                     FWYGFSEMFADHTWFEHYLDRLAQVTVDEVHRVAKAYLVKSRRTAG"
     gene            complement(1447..2781)
                     /locus_tag="AC162G_02470"
     CDS             complement(1447..2781)
                     /locus_tag="AC162G_02470"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose dehydrogenase family protein"
                     /protein_id="HIR5074710.1"
                     /translation="MKNIAVVGVGYVGLVTGTCLADLGNCVTCLDISQERIENLKQGM
                     LPIFEPGLEEVVRRNVKAGRLWFTTSYADAINGGNGMQPAEFAFIAVGTPSAVDGAAD
                     LEYVRAAAESIAEVMDHPLIIVNKSTVPVGTGDWVADIVKTRQKKPVSFSVVSNPEFL
                     REGSAIHDFMNPDRVVLGSLDREAAEKVAQLHLPLRTTLVITDLRTAEMIKYASNAFL
                     ATKISFINEIANICEELGADIKEVAAGMGFDKRIGRAFLDAGIGYGGSCFPKDVLALA
                     HMAAVKGRHPQLLQAVMDINGDQRRSILRKVSALLGGELKGKTVGLLGLAFKENTDDM
                     RDAPSIDLARWLVEQGAKVKGYDPVAMTTAASMMPPGMKFAEDPYELAADCDAVILVT
                     PWNEFKQLDLERIKQSMKMPVLVDGRNVYDPELMRALGFTYRGVGRGYNDRQ"
     gene            complement(2830..4134)
                     /locus_tag="AC162G_02475"
     CDS             complement(2830..4134)
                     /locus_tag="AC162G_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5074711.1"
                     /translation="MKVAIIGAGAAGLAAAYDLSRRRHSVTVYEAAAEVGGLAAGFKT
                     PHWDWTLEKFYHHWFASDKHILGLIKELGWSDQVLFPRPYTVIYYEGRFHPFDSIFTN
                     IPLFILRHFPLIDFIRFGLVGAYLRFWPRWQSLEKVTAVEWTRRYFGERIYRVMWKPL
                     LVSKFGEENLNVVNMAWMWARLHSRTTRLGTFKGGFQVFLDRLAGVVRSHGAAIRLST
                     PVTGIRRNADGTLSVQTAADQSSFDAVMSTSSPALMARLAPDLPAAYSERLRGLKSMG
                     AVVLILALDRPLTKYYWHNLPKEAGFPFLSLVEHTNYVSPEHYGGDHLVYCGDYLDPD
                     HEYFRLGKDELLERFLPALKRFNPAFERNWLRETWLWKTPYAQPVPPLNHSLSVPDIR
                     TPLRGLYFASMSQVYPWDRGTNYAVEIGRRAARMIMADAESV"
     gene            complement(4131..>4984)
                     /locus_tag="AC162G_02480"
     CDS             complement(4131..>4984)
                     /locus_tag="AC162G_02480"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR5074712.1"
                     /translation="AWRWQTLLKPLKVVSARRLFPVVVIGYMGNNIYPARIGELLRAY
                     VLRRNEKISISSSLATVIVERLFDGLVVLLFVFLVLPSAQAQWDLPEPMRALIAAGSL
                     LFFAALVVFLALAARPDMASRVYGFLIVRLLPHRLRAPVGGLLDRFVTGLSALRDFRQ
                     VLVIFLITLVVWLVETTTYWLVMHAFPFEVPFATLMLMNGVANLVTTIPALPGYVGTF
                     DLPGIAVLVAFGVDPEIATSYTLVLHAVLWLPITLLGLFYMVRQSLRWSDIRKAADWQ
                     DARAAAP"
BASE COUNT          937 a         1554 c         1554 g          939 t
ORIGIN      
        1 cccgccgtgc ggcggctctt caccaggtac gccttcgcca cgcgatgaac ctcgtcaacc
       61 gtcacctgcg ccaggcggtc gaggtaatgc tcgaaccacg tatggtcggc gaacatctcg
      121 ctgaagccat accagaaacc ctggttggtg acgctctcgc tgccgtacgc gaactgcgcc
      181 ctggattggc ggatggcctt gtcgacctct tcctgcgatg gcggctggtc gacgaccttc
      241 tgcagctcga cgtcgagtgc ggcttcgacc tccgccggtg tgtggccctg gcgcaccgtg
      301 gcgctgatcg cgtacatgta ggggtcgtgc gtagggacca agttgcctga tacatcggtg
      361 gccagctccg tatcgaccag cgcgcggtac agccgcgacg acgcattcgt cgtcccaccg
      421 ccgaacagcg ataaccccga tgcgccggtc aggatcgcgt ccagcacgac cagcggcgca
      481 aagtcggcgt gccgcgcctc gggcgcacga taagcgacgt cggcgtacga cgtcaggcct
      541 tcgccttcaa ccgtgatgcg ccgctcgccc ttttgctcag gctcgaccga cgcgatcttc
      601 ggcagcgccg ggccgggctt gatccgggcg aaggctttgc cgatgcgaga tagcatgtct
      661 ttggcgtcga acgcgccgac gccgatcgcc acgcagttgt tgggcgcgta gtaggtccgg
      721 tagtgcgcgt acaggtcgtc gcgcgtcatc gactgcaggt cgcacaggtg accgatcgtc
      781 tcgtggtggt agccgtgcac catgaagacg ctggcctgca gtgtttcgcc gagcaggaac
      841 tgcgggctgt tctcgctgcc ctggcgctcg gagatgatga ccgtccgctc gctctcgacc
      901 tccttcggcg cgaacagcgc gttcgtcatc cggtcggcct cgatgcggag cgccaggtcg
      961 atcttgccgg cgggcagcgt ctcgaagtac gtcgtgaagt cgagccaggt gaatccgttc
     1021 cacacgccgc cctcgcgcga cacgagcttg tcgaccgtcg cccgggggaa cgtcttggtg
     1081 cccttgaaca gcatgtgctc gacccaatgg ctgatgccgg tgatccccga tcgctcgtgg
     1141 cgtgacccga ctttgtacca gcaccaaaac gtcgcgaccg gcgcgttgtg gttctcgcgc
     1201 aggatcacgg tcaacccgtt gtccagcacg gccttgagca cgccgtttcg cgccagaatt
     1261 ttcggtctgc ggacggatgc ggtgcggcga ggcgccgcct ttctcgtctt catgcttggg
     1321 ccactcctct ggttactaga gattccacct ggattaacga cttggaatcg aggctgagat
     1381 gcttcgcatt gtagctttgg attttgaact ttgaagtttg aagtttgcac tctgtacacc
     1441 ccatgctcac tggcggtcat tgtagccgcg cccgacgccg cgataggtaa atcccagcgc
     1501 gcgcatcagc tccggatcgt agacattgcg gccgtcgacg agcaccggca tcttcatcga
     1561 ctgcttgatg cgttcgaggt cgagctgctt gaactcgttc cacggggtca ccaggatgac
     1621 ggcatcgcag tcggcggcaa gctcatacgg gtcttcggcg aacttcattc cgggcggcat
     1681 catcgaggcg gcggtcgtca tggccacggg atcatatcct tttactttcg cgccctgttc
     1741 gacgagccag cgcgccaggt cgatcgacgg cgcgtcgcgc atgtcgtccg tattctcctt
     1801 gaaggccagt cccagcagac caaccgtctt gccttttagc tcgccgccga gcagagcgct
     1861 caccttgcgc agaatgctcc tgcgctgatc gccgttaatg tccatcaccg cttgcagcaa
     1921 ctgaggatgg cgccctttga ccgcggccat gtgcgccagc gccagaacgt ctttgggaaa
     1981 acagctccca ccgtatccta tacccgcatc gagaaaggca cggccaatgc gcttatcgaa
     2041 gcccatgccg gcggcgacct cttttatatc cgcgcccaat tcttcgcaaa tgttggcgat
     2101 ctcgttgatg aagctgatct tggtcgccag gaaggcgttg gacgcatact tgatcatttc
     2161 cgccgtgcgc aggtcggtga tcaccagtgt ggtgcgcagc ggcaggtgca gttgcgccac
     2221 cttctcggcg gcctcccggt cgagcgagcc gagcacgacg cggtcgggat tcatgaagtc
     2281 gtggatcgcc gagccttcgc gcagaaactc cgggttggag acgacgctga acgagacggg
     2341 ctttttctgc ctggtcttga cgatgtcggc gacccagtcg ccggtgccaa ccggcacggt
     2401 cgatttgttg acgatgatga gcggatggtc catcacctcc gcgatcgact cggccgcggc
     2461 tcgaacgtat tccagatcgg ccgcgccgtc gaccgcactc ggcgtgccga cggcgatgaa
     2521 agcgaattcg gccggctgca tgccgttccc accgttgatc gcatcggcgt agctcgtcgt
     2581 gaaccacagg cgcccggcct tgacgttgcg ccggacgact tcttccagac ccggctcgaa
     2641 aatcggcagc atgccttgct tgagattctc gatgcgttcc tggctgatgt cgaggcaggt
     2701 gacgcaattg ccgaggtcgg ccaggcaggt gccggtgacg aggccgacgt atccgacgcc
     2761 gacgacagcg atgtttttca tggtgcaatc ctcagaaagt gaatttgatg gtgcagcaca
     2821 ggcccaattc tacaccgatt cggcgtctgc catgatcatg cgcgcagcgc gccggccgat
     2881 ctcgactgca tagttggtgc cgcgatccca cggatacacc tgcgacatcg aggcgaagta
     2941 cagaccccgc aatggtgtgc gaatgtccgg aacgctgagc gagtggttga ggggcggaac
     3001 cggttgggca taaggcgtct tccacagcca ggtctccctt aaccagttcc gttcaaaggc
     3061 cgggttgaat cgtttcagcg cgggcagaaa tcgttcgagc aattcgtctt tgcccagcct
     3121 gaagtattcg tgatcaggat cgaggtagtc gccgcagtac accaggtggt cgccgccata
     3181 atgctcgggg ctgacgtagt tggtgtgctc gaccagcgac aggaaaggaa accctgcctc
     3241 tttgggcagg ttatgccagt agtatttcgt cagggggcga tccagcgcca ggatcagcac
     3301 gactgcgccc atcgacttca agccgcgcag ccgctctgag tacgcggcgg gcaggtcggg
     3361 ggcgagccgc gccatgagcg cgggcgagga agtggacatc acggcgtcga aactcgactg
     3421 gtcggcggcg gtctgcacgc tcagcgtgcc gtcggcgttt cgccggatgc cggtcacggg
     3481 tgtgctcagc cgaatcgccg cgccgtgcga tcgcacgacg ccggccagcc tgtcgagaaa
     3541 gacctggaat cctcccttga aagtgcccag ccgcgtcgtg cggctgtgca ggcgcgccca
     3601 catccaggcc atgttcacga cattcaagtt ctcttcgcca aacttgctga ccagcagagg
     3661 cttccacatc acacggtaaa tccgctcacc aaagtagcgg cgtgtccact ccacggcggt
     3721 caccttttcc agggactgcc agcgcggcca gaagcgtaag tatgcgccga ccagcccgaa
     3781 gcggataaag tcgatcaacg ggaagtgtcg caggatgaac agtgggatgt tggtgaagat
     3841 cgaatcgaag ggatgaaatc gcccctcgta gtagatgacc gtgtatgggc gggggaagag
     3901 aacctggtcg ctccagccca attcttttat caggccaagg atgtgcttgt ccgacgcaaa
     3961 ccaatggtga tagaactttt cgagcgtcca gtcccagtgc ggggtcttga aaccggctgc
     4021 cagcccgccg acttcggctg cggcttcgta aaccgtgacg ctgtgacgcc ggcgagacag
     4081 gtcataggcg gccgccaggc cggccgcccc ggctccgatg atggccacct tcatggcgcg
     4141 gctgccctgg cgtcctgcca gtcggcggcc ttcctgatgt cgctccagcg caggctctgg
     4201 cgcaccatgt agaagagtcc cagcagagtg atcggcagcc acagcacggc gtgcagcacg
     4261 agcgtgtagg aagtggcgat ctctggatcg acgccgaagg ccaccaggac ggcaatgccg
     4321 ggcaggtcaa acgtgcccac gtatcctggc agcgccggaa tcgtcgtcac cagattggct
     4381 acgccgttca tcagcatcag cgtcgcgaac gggacttcaa acgggaaggc atgcatcacc
     4441 agccagtacg tggtggtctc taccagccac acgaccagcg tgatcaggaa aatgaccagc
     4501 acttgacgga agtcgcgcag tgcagacagg cccgtcacga atcgatcgag cagcccgcca
     4561 actggcgccc gcaggcggtg tggaagcagg cgcacgatca ggaagccgta gacccgggat
     4621 gccatatcgg ggcgcgcagc cagcgccagg aacacgacca aagccgcgaa aaacaggaga
     4681 ctgccggccg cgatcaaggc tcgcatcggt tccggcaaat cccactgagc ctgcgccgag
     4741 ggcagcacca ggaagacgaa caaaagcacc accagcccat cgaataggcg ctcgacgata
     4801 acggtggcga gtgaagaact gatgctgatc ttctcgttgc gcctcaacac gtaggcccgc
     4861 agcagctcgc cgatgcgagc gggatagatg ttgttgccca tgtagccgat cacgacgacg
     4921 gggaacaacc ttcgggcgga gacgactttc aggggcttga gcagcgtctg ccagcgccag
     4981 gccc
//
LOCUS       DBNHXB010000067         9606 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4039014, whole genome shotgun sequence.
ACCESSION   DBNHXB010000067 DBNHXB010000000
VERSION     DBNHXB010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9606)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9606)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9606
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4039014"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="AC162G_02485"
     CDS             complement(<1..256)
                     /locus_tag="AC162G_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074713.1"
                     /translation="MNAKRRPVTTKWEYRISVTGELPGYAEDIPDATALWKRKYADGS
                     SDWDTIIRIGDEGWEMVNVFPIAGPGGRTTALMFVFKRPQF"
     gene            complement(414..2126)
                     /locus_tag="AC162G_02490"
     CDS             complement(414..2126)
                     /locus_tag="AC162G_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HIR5074714.1"
                     /translation="MSSLVFPAQPRQTVQVRFDNGMGTFEAPAGTPLEVFARAAQPDV
                     FDLAVAAIVNGKLGELNTPITRDSEATLITRASHDGSRIYRRSLTFLMIVAAHELFPG
                     ARTLVDHAVPYGGYYCEIADRKPLPRADIERLRERMHEIVEADEPIVKRRVPLAEAED
                     IFKRQGYQDKLRLLRYRQKDYLTVYTLRGLTDYFYGYMLPSTGYLRAFNLIEFPPGFV
                     LQFPRASQPATLSTPVESPKLSAVFRRHAEWMKIMDVEDVGALNAAIESQRVREIMLV
                     NEALHEQHVAEIAGTIAAQRDTIRLVLIAGPSASGKTTFAKRLAVQLLAHSLRPIAIG
                     MDDYFVDRERTPRCPDGTYDFEAFDAVDHPLFNRHLLELTEGRDVQLPHFDFKTGARQ
                     PGRRVRITRDHIILIEGIHALNPRLLPDVPPERVCRVYVSALTQLNIDDHNRVATTDT
                     RLVRRIVRDARDRGYSARDTIGRWNKVRDGETRNIFPYQENADFMFNSGLVYEHAVLR
                     PIAEPLLRQVRPDTLEYIEVARLLAALDWFLPCPADFVPDNSILQEFINPERNIFKTL
                     TFRV"
     gene            complement(2213..2803)
                     /locus_tag="AC162G_02495"
     CDS             complement(2213..2803)
                     /locus_tag="AC162G_02495"
                     /inference="COORDINATES: protein motif:HMM:NF017496.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR5074715.1"
                     /translation="MSVIIRDYMTDEEFFDISPDDRKAELIDGEMIVTSPASEIHERV
                     FIFLATVLEMFVAERDLGIVRGSRTAMRLSEKEVYEPDILSVAKDRLSLVHDNYIDGP
                     ADLVVEILSRSTAYFDRGHKLANCALAGVREVWLIDPGKRTAEFYRLIGARYEPIPVD
                     TEGVFRSQAMPGFWLRTAWLWEQPKPLQVARELGLV"
     gene            2866..3087
                     /locus_tag="AC162G_02500"
     CDS             2866..3087
                     /locus_tag="AC162G_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074716.1"
                     /translation="MKISVVGPSLAGVAARAAGRSPGLGARRYLELSILKPDAYVVQG
                     FSDVMPRGFGDKLTGEELDALLAFLLTLK"
     gene            complement(3143..4663)
                     /locus_tag="AC162G_02505"
     CDS             complement(3143..4663)
                     /locus_tag="AC162G_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR5074717.1"
                     /translation="MSPNDSTKVRIRMYRQGLGDCLLLTFFTGPEPIHMLIDCGTLGA
                     TTTGVTMSEVVADIAVATGRHLHLLVVTHEHKDHVSGFAGQGKLFEADGENPRFTVDR
                     VWAAWTEDPNDDLAKQVARFKKDLLASVQLAGEVLHANQASLEDEREALRGIASGVRE
                     LLGFYGDVPDGDAPLGVDFAKTVHEAMSYASMRAGSLPDFLSPGRVIEPVWLPGVRFY
                     VLGPPRRKEDLVVLGDHDSPELYGLTQRLSADLARNIPFYTSDQPIASYLNKLGESDA
                     DARQAFEAGLPFDTCFRIESSDEAARARYFQSYDAPGEAWRRIDYDWLTAADDLALQL
                     DNATNNTSLVLAIELIGDGRVLLLPADAQLGNWKSWEKVRFSVTESGTTRTVKAADLL
                     KQTVLYKVGHHASHNATMKDKGLEAMERDDLVAMIPVDRRVALNKKPPWQMPAQALYK
                     RLLQKTQGRVLRSDTGWPRNSDRPSGVTDAEWAEWQAARTDENISIEQLYIEYRLH"
     gene            complement(4703..6589)
                     /locus_tag="AC162G_02510"
     CDS             complement(4703..6589)
                     /locus_tag="AC162G_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007636285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074718.1"
                     /translation="MPSETAKYVSPPIYRTLRGYAIDPSLAQQLETAPVSQILFKLPW
                     EELKPGPVGEYLEVIDYDPASKCFYEPVNLDEPYLLAQDGLTPSEGTPQFHQQMVYAV
                     VRLTIHNFERALGRKALWSPGPSPDPKRPWDDSNYVQRLRIYPHALREPNAYYSPAKK
                     ALLFGYFPASADQADGSGNHVPGGMVFTCLSHDIVAHETTHALLDGLHRRLNRATNPD
                     MLALHEAFADVVALFQHFTFPEILRHQIARTRGDIRAQESLLGQLAGQFGRATGTRSA
                     LRDAIGHLDTKTGRWEPHMPDPAEYERTFEPHQRGAILVAAIFDAFLSIYNNRITDLL
                     RLYTGGSGVVPAGAIHPDLVQRLSEEANKAANHVLAMCVRALDYCPPVDLTFGEYLRA
                     LVTADADLVRDDDRKYRVAFAEAFRRRGIYPRGVRTLSVENLLWRASDNDDRQPSEEL
                     IKILGGLREFGHRQLYASSRKQIFDQARDMRRRLHDQIEAHFKSGNTGVQDGLFLGLD
                     VAGRTFEVHSLHFASRVGPDGDLLLQAIVQITQDMDLPVDTADQEQGRMRFQGGCTVV
                     VDLEGPRIAYCVRKPILSLTRRERQRDFIQRVARATLRATYLDERDFRERREPFALLH
                     RGGG"
     gene            complement(6858..>9606)
                     /locus_tag="AC162G_02515"
     CDS             complement(6858..>9606)
                     /locus_tag="AC162G_02515"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5074719.1"
                     /translation="DLRRLLVNDPACRLVTLVGPGGIGKTRLAVAAAEEQLSRLRSTP
                     EIGPLFPDGVYFASLAPLSAGEQMVPAIANTLGFSFYSAKPPREQLLDYVRPKRMLLV
                     LDNLEHLLDGEGVRLVTDILAMAPHLKLLVTSRVRLNVQGEQVYPVAGMSVPEAEITI
                     GGPERDEQGLVVGAETYSAVQLFQQSAWRVRPDFRLTADNLPAVIRICQLVQGMPLGI
                     VLAVPWLVVLPADEIAAEIQRGLDFLRTDMHDLPERQRSLRSIFDASWKLLTDQERAA
                     LLGLAVFQGGSTREAAQDVAGASLETLLALVNKSWLQPEAGGRYQLHELLRQYAAEKL
                     QSDPVYWQQCRQRHSAYFAERLRQLDRQMRGTQPEVAFAAVTADFANIRAAWSWLVEQ
                     GWFDTVVEQMLPALYRYGEGRLQALALQPLLATAQQAVANADPTTADRRYRAILLTAQ
                     AAFLKTGTPVRSLILEVATPAYEDAIQQAWSIAGASDSLLAMGFWGALLIFIYGWIFD
                     EEEGLRHLRQLLLPLRQAGQRWELAFALQNLGRLIYTRLPDEAPDQHLAEALQHLTEA
                     LALFQELRDELESGCTLRLLGVVRMMQRHFPEARDHLLAAQAKLEACGDQANAASINW
                     LLADIHLRLGEYETAFGYLRRMRQAYADRSLQSDALSRESYEALRYSTIDHAKETREQ
                     TLALVREAGDDFSLAWSTWEMGEIYRVAGDYLTARQWYEQARVLFEKAQDKSGNIFYH
                     RGLGDIAHALGDYGEAQRQFQASLNATQQIKHEWGAAYALAGLGRASLALGEHELAHR
                     HFLEALRKAYKIGDGGILMFVITGIAGLYAACGQEERAVELCALVAHQPMSWRETKEQ
                     AAAVLAAAAAALPPERLSAAQEGSHGSDIWKTAERVRQEMSSSTFWSASHVAGFTSET
                     T"
BASE COUNT         1720 a         3000 c         3077 g         1809 t
ORIGIN      
        1 ggaattgggg acgcttgaac acaaacatca gggctgtagt ccgaccgccc ggcccggcaa
       61 tggggaagac gttgaccatt tcccatcctt catcgccaat cctgatgatg gtatcccagt
      121 cgctcgatcc gtctgcgtac ttgcgcttcc acaacgcggt cgcatcgggt atgtcttccg
      181 cataacccgg aagttctccc gtcaccgaga tgcggtactc ccacttcgtc gtgactggtc
      241 gccgcttggc attcatcgtc tgcctccttc acggcatctg gccgggcgat ctcgcgccgg
      301 tgcgcgacgg ccgccgcgcc gattcacgtc atgtaaccgc catagaggtg cgtaaacggc
      361 gaggagtata gaccggatag tcaggcagcg caatcgtttc gcggggtttg ccttcaaacg
      421 cggaaagtca gcgtcttgaa gatattgcgc tcgggattga taaactcttg caggatcgag
      481 ttgtccggca cgaaatcggc agggcagggc aggaaccagt cgagcgcggc gagcaggcgc
      541 gcgacttcga tgtactccag tgtgtcgggc cgcacttgcc gcagcagcgg ctccgcgatc
      601 ggcctgagca cggcatgctc gtacaccagc cccgaattga acatgaaatc ggcgttctct
      661 tgatacggga agatgttgcg cgtctcgccg tcgcggactt tgttccagcg cccgatcgtg
      721 tcgcgcgccg agtagccgcg atcgcgcgcg tcgcgcacga tgcgccgcac cagccgcgtg
      781 tcggtcgtcg cgacgcgatt gtggtcgtcg atgttgagct gcgtcagcgc ggacacgtac
      841 acgcggcaga cacgctcagg cggcacgtcg ggcagcagtc tcgggttgag cgcgtggatg
      901 ccttcaatca gtatgatgtg gtcgcgcgtg atgcgcaccc ggcgtccggg ctggcgcgcg
      961 ccggttttga agtcgaagtg cggcaactgc acgtcgcgcc cctcggtcag ttccagcagg
     1021 tggcggttga acagcgggtg atccaccgcg tcgaatgcct cgaagtcgta tgtgccgtcc
     1081 gggcagcgcg gcgtgcgctc gcgatcgacg aagtaatcgt ccatgccgat cgcgatcggg
     1141 cggaggctgt gcgccaggag ttggacggcc agccgcttgg cgaaggtggt cttgcctgac
     1201 gccgacggcc cggcgatcag caccagcctg atcgtgtcgc gctgcgcggc gatcgttccg
     1261 gcgatttcgg cgacgtgctg ctcgtgcagc gcctcgttga ccagcatgat ttcgcggacg
     1321 cgctgcgact cgatggccgc gttcagcgcg ccgacgtctt ccacgtccat gatcttcatc
     1381 cactcggcgt gccggcggaa cacggcgctc agcttggggg attcgacggg cgtcgacagc
     1441 gtggccggtt ggctggcgcg cgggaactgc agcacgaacc ctggcgggaa ctcgatcaga
     1501 ttgaaagcgc gcaggtaacc ggtcgacggc agcatgtagc cgtagaaata gtcggtgagg
     1561 ccgcgcagcg tgtagacggt caggtagtct ttctgccggt agcgcagcag gcgcagcttg
     1621 tcctggtagc cctgtcgctt gaagatgtcc tcggcttccg ccagcggcac acgccgcttg
     1681 acgatcggct cgtcggcttc gacaatctcg tgcatgcgct cgcgcagccg ctcgatgtcg
     1741 gcgcggggca gcggcttgcg gtcggcgatc tcgcagtaat agccgccgta cggtaccgcg
     1801 tgatcgacga gcgtccgcgc accggggaat agctcgtgcg cggcgacgat catcaggaag
     1861 gtgagcgagc gccggtagat gcggctgccg tcgtgcgagg cgcgcgtgat cagcgtggct
     1921 tcggagtcgc gcgtgatcgg tgtgttcagc tcgccgagct tgccgttgac gatcgcggcg
     1981 acggcgaggt cgaacacgtc gggctgggcc gcgcgggcga agacctccag cggcgtgccg
     2041 gccggcgctt cgaacgtgcc catgccgttg tcgaagcgaa cctggacggt ctggcggggt
     2101 tgggcgggga agacgaggga ggacataacg agccagtgaa tcagcgagtc agcgagttgc
     2161 gcattggcga atcaacgagg cagaacacct atgtgagtgg tttgaggcgg gatcaaacca
     2221 ggccgagttc gcgggcgact tgcaggggtt tgggctgctc ccacagccac gccgtgcgca
     2281 gccagaaacc gggcatggcc tgcgagcgaa agacgccttc agtgtcgacg ggaatcggct
     2341 cgtatcgcgc gccgatgagc cggtagaact cggcggtgcg cttgccggga tcgatcagcc
     2401 aaacttcgcg cacgccggcc agcgcgcagt tggcgagctt gtggcctcgg tcgaaataag
     2461 ccgtgctccg cgacaggatc tcgacgacca ggtcggcagg cccgtcgata tagttgtcgt
     2521 gaaccaggct caagcgatct ttggcaacgg agagaatatc cggctcgtac acttccttct
     2581 cactcaaacg catggcggtg cgcgatccgc gcacgatgcc caggtcgcgt tcggcgacga
     2641 acatttccag aaccgtggcc agaaagataa agacgcgctc gtgtatttcg gaagcgggtg
     2701 aagtcacgat catctctcca tcgatcagct cggctttgcg gtcgtcgggg ctgatgtcaa
     2761 agaactcttc gtccgtcatg taatctcgaa taatgacgct catggtgcac tccttacgca
     2821 tgtgagtata gttcgccgat ggatcgctgt caaggagaaa agcctttgaa aatctccgtc
     2881 gtcgggccgt cgctggccgg tgtggcggcg cgcgctgcgg ggcgctctcc cggcctcggc
     2941 gcgcggcggt atctggagct gtcgatcctc aagccggatg cctacgtcgt ccagggcttc
     3001 tcggatgtga tgcccagggg ctttggggat aagctgacgg gagaagagtt agacgcgctg
     3061 ctcgcgtttc tattgacgct gaagtgatgc gtaatgaggg atacgtgatc acgtgatcac
     3121 gtaatcacga tatcactcat cgtcagtgca atcggtattc aatgtagagc tgttcaatgc
     3181 tgatgttctc gtccgtgcgc gcggcctgcc attccgccca ttctgcgtct gttacgcccg
     3241 acggccggtc gctgttgcgc ggccagccag tatcggagcg tagcacgcgc ccttgcgtct
     3301 tctggagcag ccgcttgtac aacgcctgcg cgggcatctg ccacgggggt tttttattca
     3361 gcgcgacccg acgatctact gggatcattg cgaccaggtc atcgcgctcc atggcttcca
     3421 gtcccttgtc cttcatcgtc gcattgtgac tggcgtggtg accaaccttg tagaggacgg
     3481 tttgcttcaa caggtctgcc gccttcaccg tgcgcgttgt gccgctctcg gtcacgctga
     3541 atcttacctt ttcccatgac ttccagttgc ccagttgtgc atcggctggc agcagcagga
     3601 cgcggccgtc gccaatcaac tcgatggcga gcacgaggct tgtgttgttg gtggcgttgt
     3661 caagctgaag cgcaaggtcg tcggctgcgg tcagccagtc gtagtcaatg cgccgccagg
     3721 cctcgccggg agcgtcatac gactggaaat agcgcgcccg cgccgcctcg tcgctgctct
     3781 cgatccggaa gcaggtgtcg aacggcagac ccgcctcgaa cgcctgccgg gcatcggcgt
     3841 ccgattcgcc gagtttgttg agatagctgg cgatgggctg atccgacgtg tagaagggta
     3901 tattgcgcgc gaggtcggcg ctcaggcgct gggtgaggcc ataaagctcg ggagaatcgt
     3961 gatcgcctag caccaccagg tcctctttgc ggcgaggcgg gccgagcaca taaaatcgca
     4021 caccgggcag ccacacaggc tcgatgacgc ggccaggcga caggaagtcg ggcaaactgc
     4081 ccgcacgcat cgaggcatag ctcatcgcct cgtggaccgt cttagcgaag tcgaccccca
     4141 gtggcgcgtc accgtccggg acgtcaccgt aaaagcccaa gagctcgcga acgccagacg
     4201 cgatgccgcg caacgcctcc cgctcatctt ccagagacgc ctggttcgcg tgcagcacct
     4261 cgccagcgag ttgaacggaa gcgagcagat ccttcttgaa ccgggcgacc tgcttcgcca
     4321 gatcgtcgtt tgggtcctcc gtccaggcgg cccacaccct gtccaccgta aaccgcgggt
     4381 tctccccgtc ggcttcgaag agcttgccct ggcccgcgaa gccggaaaca tgatctttgt
     4441 gttcgtgcgt gacgaccagc aggtggaggt gccgcccggt ggccacggcg atatcggcaa
     4501 ccacttccga catcgtcacg ccggtggtgg tcgcaccgag tgtgccgcag tcaatcagca
     4561 tgtggatggg ttcgggtccg gtgaagaacg tcagcaggag gcagtcgccc agaccctgcc
     4621 gatacattcg gatacgtacc ttggtcgagt cattcggtga catagaacct ccggggcgtt
     4681 accgtttgcc tttgcctcag cctcagccgc cgccgcgatg aagcagcgcg aacggctcgc
     4741 ggcgttcacg aaagtcgcgc tcgtcgaggt acgtcgcgcg cagtgtcgcg cgcgcaacgc
     4801 gttggataaa gtcgcgctgc cgctcgcggc gtgtcaagct taagatcggt ttgcggacgc
     4861 agtaggcgat cctcggtccc tcaaggtcca cgacgaccgt gcagccgcct tggaagcgca
     4921 tccgcccctg ctcttggtct gcggtgtcca caggcaggtc catgtcctgc gtgatctgca
     4981 caatcgcctg caacagcaag tcaccgtccg gtccgacgcg gctcgcgaag tgcaacgagt
     5041 gcacctcgaa agtgcggccg gccacgtcga gcccgaggaa caggccgtcc tgaacgccgg
     5101 tatttccact cttgaagtgt gcctcgatct ggtcatgcag tcttcgccgc atgtccctgg
     5161 cttggtcaaa aatctgctta cgacttgacg cgtaaagctg ccgatgcccg aactcacgaa
     5221 gcccgccgag tattttgatc aattcctccg acggctggcg atcgtcgttg tcggatgcgc
     5281 gccacagcag attctcgacg gagagcgtcc gcacgccgcg cgggtagatg ccgcggcgac
     5341 gaaaggcttc ggcaaaggcc acgcggtact tgcggtcatc gtcgcgcacg agatcggcgt
     5401 cggcagtgac aagggcgcgc aggtactcgc cgaaggtcag atcaaccggc gggcagtagt
     5461 ccagcgcgcg cacgcacatc gccagcacgt ggttggccgc cttgttggct tcttcgctca
     5521 agcgctggac cagatcgggg tgaatcgcgc cggctggcac caccccacta ccgcccgtgt
     5581 acaggcgcag caggtcggtg atacggttgt tgtagatcga aaggaaggcg tcgaagatcg
     5641 cggcgacgag gatggcgcca cgctggtgcg gctcgaaggt ccgctcatat tccgccgggt
     5701 caggcatgtg cggctcccat cgccccgttt tggtgtcgag atgtccaatc gcatcgcgca
     5761 gcgcactgcg cgttccagtc gcgcggccga actgccccgc gagctgaccc agcaacgact
     5821 cctgcgcgcg gatgtcgccg cgcgtgcgtg caatctggtg gcgcagaatc tcgggaaagg
     5881 tgaaatgctg gaacagcgcg acgacgtcag cgaaggcctc gtgcaaggca agcatgtcgg
     5941 gattcgtggc gcggttgagg cggcgatgca ggccgtcgag cagcgcgtgt gtcgtttcgt
     6001 gcgcgacgat gtcgtgcgaa aggcaggtga ataccatgcc gcccggaacg tggttgccag
     6061 agccgtcggc ctggtcggcc gaggcgggga agtatccgaa cagcagcgcc ttcttcgccg
     6121 ggctgtagta ggcgttcggc tcgcggagcg cgtgcggata gatgcgcagc cgctggacgt
     6181 agttggagtc atcccagggc ctcttgggat ctgggctggg accgggcgac cacagcgcct
     6241 tgcgtccgag cgcgcgctcg aagttgtgaa tcgtgagccg caccaccgca tagaccatct
     6301 gctggtgaaa ctgcggcgtg ccctccgagg gggtcaagcc atcctgcgcg agcaggtacg
     6361 gctcgtcgag attgaccggc tcgtagaagc acttgctggc tgggtcgtag tcaatcactt
     6421 ccaggtactc gcccaccggc cctggcttca gctcctccca cgggagcttg aataggatct
     6481 ggctgaccgg agcggtctcg agttgctggg cgaggctggg atcaatcgcg tagccgcgca
     6541 gcgttcgata gatgggcggc gatacgtact tcgctgtctc ggatggcatg gataacctcg
     6601 cgagcatgac tgcctcgacc ttccgcagat tatataccca aaacatggcg ggcaaggcgc
     6661 accgacatcg actgccgagg gggtcgtcgt cgattacagc gacatcacgc tgcgcggcat
     6721 ttcgaacggg aagggagcgc cgccgcccgg cgcgaacgac agtggcgctt tggccgtgct
     6781 aacgctgcac gcgctgagtg aaggcaacag tagcctcgtc ttccaagcgg cgcaggtcag
     6841 cgatcgagca gcgcggctca tgtcgtttca gaggtgaaac cggccacgtg cgacgcactc
     6901 caaaaggttg agctggacat ttcctggcgg acacgctcgg ctgttttcca gatgtcgctg
     6961 ccgtggctgc cttcctgcgc cgcgctgagg cgctccggcg ggagcgctgc ggcagccgcc
     7021 gcaagtacgg ctgccgcctg ctcctttgtc tcacgccaac tcatcggctg atgagcgacc
     7081 agcgcgcata gttccacggc tctctcttct tggccacagg ctgcgtacag gccggcgatg
     7141 cccgtaatca cgaacatgag gatgccacca tcgccgatct tgtaggcctt tcgcagcgct
     7201 tcaagaaaat gccggtgcgc caactcgtgt tctccaagcg ccaacgacgc gcgccccagt
     7261 ccggcgaggg cataggccgc tccccactcg tgcttgatct gctgcgttgc attcagactt
     7321 gcctgaaact ggcgttgggc ttcgccatag tcgcccaggg cgtgggcgat gtcgcccaaa
     7381 cctcgatgat agaaaatgtt gccgctcttg tcttgcgcct tttcaaacaa gaccctggct
     7441 tgctcatacc attgacgcgc ggtcaggtag tctccggcca cccggtagat ctcacccatt
     7501 tcccacgtgc tccaggccaa gctgaagtca tcccctgcct cgcgcaccag ggcgagcgtc
     7561 tgctctcggg tttctttggc atggtcaatc gtgctgtagc gcagggcctc gtagctctcc
     7621 ctcgacaggg catcgctctg caagctcctg tccgcgtacg cctgtctcat ccgccgcaga
     7681 tagccgaagg ccgtctcata ctcgcccagg cgcaggtgga tgtcggccag cagccagttg
     7741 atgctcgccg cgttcgcctg gtcgccacag gcctccaact tcgcttgagc cgccagcaga
     7801 tggtctctcg cttccggaaa gtgccgctgc atcatgcgaa caacgccgag caagcgcaag
     7861 gtgcaaccgc tctctagttc gtccctcaac tcctggaaaa gcgctagcgc ttccgtcaaa
     7921 tgctgaagcg cttcggcgag gtgttggtcc ggcgcttcat ccggcaatcg tgtgtagatc
     7981 aagcgcccca ggttctgcag cgcaaacgcc agttcccaac gctggccggc ctgccgaaga
     8041 gggagcaaca actggcgcag gtggcgcagg ccttcctcct cgtcgaagat ccagccatag
     8101 atgaagatca acaaggcacc ccagaaaccc atggccagca ggctatctga cgcgcccgca
     8161 atcgaccacg cctgctggat agcgtcctcg taggcgggag tggccacctc aagtattaag
     8221 gaccgcaccg gagtgccggt tttgaggaac gcggcctgtg ccgtcagcag gatggcccgg
     8281 taacgccggt ccgccgtcgt ggggtcagcg ttggccacag cctgttgggc agttgccaac
     8341 aggggctgga gcgcgagcgc ttgcaagcgc ccctcgccat accggtatag cgccggtagc
     8401 atctgctcga ccacggtatc gaaccacccc tgttcgacca gccagctcca ggctgcccgg
     8461 atattcgcga agtcagccgt cacggcggcg aaagctacct ccggctgcgt gcctctcatc
     8521 tgcctgtcca gttggcgcag gcgttcagcg aaataggcgc tatgccgctg gcggcattgc
     8581 tgccagtaaa ccgggtcaga ctgcagcttt tcggccgcgt actggcgcag aagctcatgg
     8641 agctggtagc ggccgccggc ctcgggctgc agccaggatt tgttcaccag cgccagcaac
     8701 gtctccaggc tagcgcccgc cacgtcctgc gccgcttcgc gcgtcgagcc tccctgaaaa
     8761 acggccaggc ccaggagcgc cgcgcgctcc tgatccgtga gaagcttcca ggaagcgtcg
     8821 aagatggagc gcaggctgcg ctggcgctct ggcaagtcgt gcatgtcggt cctgaggaag
     8881 tccaggccgc gttggatctc ggcggcaatc tcgtccgccg gcagcacgac cagccagggc
     8941 acggccagca caatccccaa cggcatgccc tgcaccaact ggcaaatgcg gatcacggcc
     9001 ggcaagttgt cggctgtgag ccgaaagtcg ggccgcactc gccacgcgct ttgctgaaag
     9061 agctggacgg cgctgtatgt ctcggcccca acgactaaac cctgctcgtc acgctccggc
     9121 ccaccgatag ttatctctgc ttccggcacg ctcatcccgg ccaccggata cacctgctcg
     9181 ccctgaacgt tcagccgtac acgcgatgtc accagtaact tcaagtgcgg cgccatggct
     9241 agaatgtcag ttaccaggcg cacgccctca ccgtccagca agtgctcgag attatctaac
     9301 acaagcagca ttcgcttcgg gcgcacgtag tcgaggagtt gttctcgcgg cggcttcgcg
     9361 gaatagaacg agaagccgag ggtgttggca atggccggaa ccatctgttc gcccgcgctg
     9421 agaggcgcca gcgatgcgaa gtatacgcca tcagggaata gcggacctat ttctggtgtg
     9481 gaacggaggc ggctcaactg ctcctcagcc gctgcaacgg ccaggcgcgt cttgccgata
     9541 ccgccagggc cgacgagcgt caccaggcga caggcgggat catttacgag caaccggcgg
     9601 agatcg
//
LOCUS       DBNHXB010000068         7182 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_516150, whole genome shotgun sequence.
ACCESSION   DBNHXB010000068 DBNHXB010000000
VERSION     DBNHXB010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7182)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7182
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_516150"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(381..1442)
                     /locus_tag="AC162G_02520"
     CDS             complement(381..1442)
                     /locus_tag="AC162G_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5074720.1"
                     /translation="MLAACGPSQLEQRVVIVVATPTADPAPVVVALPTDTPTPSPTPK
                     PPIRQLTAGGCCAQPFWSPDSKQVRFIDKPSPDAPVGVYAVDVGEPPGEPFLALDLIG
                     LYSNDLRLIAYPEGRDTIVEQLDNGERWVIPNQGQAVVFSPEGRRVAWEIEDIDGPFD
                     QRRTDIYLSRYTGEDPVRVTTVYGGGLHGWLDEGRILFSGRPSLDVRERTLTVLVLTT
                     NVAVDLVTAERIAGVAASPDGEWVAYFISFNEDPGRSGIWVQRTDGPEAHRLDFWGAY
                     QWRDGTRLLYTPVRESPDEPFVIWEYDTASGRSRSVTDPVTAPIQIANGDWRVSPDGR
                     YVVYVNSTDRNLWLVELAP"
     gene            complement(1571..2302)
                     /locus_tag="AC162G_02525"
     CDS             complement(1571..2302)
                     /locus_tag="AC162G_02525"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR5074721.1"
                     /translation="MHAHKRWLAGVLACLLVASLLTAPRRPATAQESTPAPERRPFIL
                     PVTDPPGPGTWYLGQAYGNTVGAYFQRDTTYRAGQGLHFGLDFAMPCGTRLVAIGDGM
                     VAKVDAPEHGSRPHNLLIRFDAGYVALYGHLSERPNLQVGQRVKQGEFVALSGDSFGT
                     CHSAPHLHLEIRSPSYTIAYNPVELIEADWDSLALIGAFSRGFERDLDNPRQWQSLDD
                     QPDTLFGGPLLNNYAKPWPPDWFRR"
     gene            2582..2824
                     /locus_tag="AC162G_02530"
     CDS             2582..2824
                     /locus_tag="AC162G_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074722.1"
                     /translation="MTTSQQYEWTVVKSHWCPAANAEVALIEKRVYPTADFLGTESFR
                     VVEQRCTHDVECNLYDHVHCKWAGTNPYNDPFAESQ"
     gene            2966..3304
                     /locus_tag="AC162G_02535"
     CDS             2966..3304
                     /locus_tag="AC162G_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008655334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5074723.1"
                     /translation="MSTTYTYAADLARRLPDVPADSIISHTLYSGDDLKATLFGFAAG
                     QELSEHTASQSAILHFVEGEADLTLGADTMSAQAGTWVHMPAHLAHSIRARTRVVMLL
                     LLLKTSKSDF"
     gene            3409..4527
                     /locus_tag="AC162G_02540"
     CDS             3409..4527
                     /locus_tag="AC162G_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074724.1"
                     /translation="MNTTGLRGHLPLLGAAMLALLAGLWAGLIRIGWQWPAWQPALPF
                     LHGPLMISGFLGTLISLERAVALSAAARPGARRVWTYLAPLLTGLGGIVLLLGLPNEI
                     GRALITLGSFVLLVAFGLIVRLRPSWDTATMAAGALLWFLGNLLWLSGRSTGKAAVWW
                     IGFLVLTIAGERLDLARVLLLRRRALITHLAGIAVFLAGLALTLWDFLSGVRVSGAGL
                     IAMGAWLLAHDVARRTIRQSGLTRFIAACLLPGYLWLLFAGGLWLFGNFSAGQVYDAA
                     LHSVLLGFVFSMIFGHAPIILPAILNLPLAFHASFYAHLLLLHVSLVIRIIGDVAASP
                     SLRMWAGLLNAIAILLFLFNTMRTFRSARSQRPSASTT"
     gene            4544..4957
                     /locus_tag="AC162G_02545"
     CDS             4544..4957
                     /locus_tag="AC162G_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR5074725.1"
                     /translation="MPTLTRWFVKSALIYFVAALTTGVVLAARPAFDLPAALAALNPV
                     YFHLLMVGWITQLIFGIVHWMFPKYSREQPRGSDRLAWATYVLLNIGLILRAIGEPMQ
                     AAQPEGAWGWLLAISAMLQWLAGVAFVANTWGRVK"
     gene            5058..5465
                     /locus_tag="AC162G_02550"
     CDS             5058..5465
                     /locus_tag="AC162G_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074726.1"
                     /translation="MPRLSRLAIRAALLYLLLGFTFGGLMLLNKGIPLAPWAWRLLPA
                     HVEFLLIGWTAQFILGVGFWILPRFSRGPARGNELFAWLAFGLVNAGVCLAGLAPVLN
                     APPSIALLGRLAEAGAAIAFAMHVWPRVKPLAQ"
     gene            5599..7065
                     /gene="tilS"
                     /locus_tag="AC162G_02555"
     CDS             5599..7065
                     /gene="tilS"
                     /locus_tag="AC162G_02555"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR5074727.1"
                     /translation="MDVLAEIRRAVRRFDLLPRGDTVVVGVSGGPDSLCLLHTLNTLR
                     GEFGVELHLAHLNHGLRGAEADADAQFVSALAAQWGLLCTVARRDVVALAREHKLSIE
                     EAARHARYAFLAETAQRVDSHTVAVAHNADDQAESVLMHFLRGSGTAGLRGMLHQSDL
                     LEFRILNSEFRIENSELKLIRPLLAVPRADIERYCREHDLRPRFDTSNLDTTFFRNRL
                     RHELLPLLETYNPNIRQALRRTADVVTAEVEILRDASEQAWRVAAISETGEAVTFNLS
                     AWRDLPPAMQRATLREAIHRLRPSLRNINFVHVDDAVNQVNAARTGARASLPQRLMLS
                     VNYDAFTVADSGYEPDLPDWPLLEVGQIVSVTLSGITPLPASEWSLEAVALDKWGDEV
                     FANPDPWTAYLDGEAAGFDVSIRTRAPGDEFHPQGMPSPVRLTNWMTNVKIPRRARDR
                     LPLIVAGGRIAWVAGCRVGQPFTVRARTQSVLRLSFVR"
BASE COUNT         1226 a         2328 c         2268 g         1360 t
ORIGIN      
        1 tgcagcttgc cggagccaac gtgcgtgtct ttgctcgtgg acctgaactc gccgacggtg
       61 ctttggttaa tcaccgctct ccccgagaac aggctcacac ctcccactga gcggatagac
      121 acgtaagcga tcaagcgatc cagcgactcg ttcttcatag tagacctcct ggctggctac
      181 aaaaggcacg acccctagaa ggcgcccctc tggccttctc ttctcgcctg cggcacaaac
      241 cggagcggct gtgggatgcg aacatatctt gataccagat tattatatgc gctgggaagc
      301 aagagtcaag caaaggtgtt acgcactctt gccatcgcaa tgaagcggag ggggttagag
      361 gtgcgtcgca ccttttcctc ctatggtgcc agctccacca accacaagtt gcgatctgtg
      421 ctgttgacgt ataccacgta gcgcccgtcc ggcgagactc tccaatctcc gttcgcgatc
      481 tgaatcggcg cggtgacggg atcggtcaca ctacgcgacc ggccactcgc cgtatcatac
      541 tcccaaatca caaacggctc gtccggcgac tcgcgaaccg gggtgtacag cagtcgcgtg
      601 ccgtcgcgcc actggtacgc gccccagaaa tccagcctgt gcgcctcggg gccatccgtg
      661 cgctgaaccc agatgccgct gcgcccggga tcttcgttga atgagataaa gtaagctacc
      721 cattcgccgt ccggcgaagc tgccacgccg gcgatccgct cagccgtcac cagatcgacc
      781 gcgacgttcg tcgtgaggac gagcaccgtc agcgtgcgct cgcgcacgtc gagcgacggg
      841 cggccgctga acagaatgcg tccttcgtcg agccagccat gcaggccgcc gccgtacacc
      901 gtcgtcacgc gcaccgggtc ttcgccggtg tagcgcgaca ggtaaatgtc ggtgcggcgc
      961 tgatcgaacg gcccgtcgat gtcttcgatt tcccaggcca cacgcctgcc ctccggcgag
     1021 aacacgacgg cttgaccttg attcgggatc acccagcgct cgccattatc caactgctcg
     1081 acaatcgtgt cgcggccttc gggatatgcg atcaacctga gatcgttcga atacaggcca
     1141 atcagatcga gagccaggaa tggctcgccg ggcggctcac ccacatcgac ggcgtaaact
     1201 cctaccggcg catccggcga aggcttgtcg atgaatctca cctgcttcga gtcgggcgac
     1261 cagaagggct gcgcgcagca gccgcccgcc gtcagttgcc tgatcggcgg ctttggcgtc
     1321 ggcgaaggcg ttggcgtatc ggtgggaagg gcaacgacga caggcgccgg atcggccgtc
     1381 ggagtggcga cgacaatcac gactctctgc tcaagctggc tcggcccgca ggccgccagc
     1441 atcacaagaa tcgcagagaa cacgacggtt aagagatttg tcagccgcgg attaacgcgg
     1501 attgcgcgga ccagaatctt tagtccgcgt tcatccgcgt tcatccgcgt ccaaaattgc
     1561 ttgtaagcca tcatctgcgg aaccaatcgg gcggccaggg cttggcgtag ttgttaagaa
     1621 gtgggccgcc gaataaggta tcgggttgat cgtcgaggga ttgccactgg cgcgggttgt
     1681 ccaggtcgcg ctcgaagccc cggctgaacg cgccgatcag cgccagcgaa tcccaatcgg
     1741 cctcgatcag ctcgaccggg ttgtacgcaa tcgtgtagct ggggctgcga atctccaggt
     1801 gcaggtgcgg cgcggagtgg cacgtgccga acgagtcgcc gctcagcgcg acgaactcac
     1861 cttgcttcac gcgctgaccg acctgcaggt tcgggcgctc agacaggtgg ccgtacagcg
     1921 ccacgtagcc ggcgtcgaac cggatgagca gattgtgcgg ccgcgatccg tgctccggcg
     1981 cgtcgacctt cgcgaccatc ccgtcgccga tggcgacgag ccgtgtcccg cacggcatgg
     2041 cgaagtcgag gccgaaatgc aagccctgcc cggcacgata ggtcgtgtcg cgctggaagt
     2101 acgcgccgac ggtgttgccg tacgcttgac ccagatacca ggtacccggt ccgggtggat
     2161 cggtcaccgg cagtataaac ggcctgcgct ccggcgcggg cgtcgattcc tgcgcggtgg
     2221 ccggacggcg cggagcggtc agcagcgagg cgacgagcaa acacgccaac acaccggcaa
     2281 gccatctctt atgcgcatgc atccctgcaa tctccaggtg gactggggca agagcataat
     2341 tcggcgcagg cgagttgtca actggcgggt tatagggcga aacggggtga aagccgtcac
     2401 aggcggcagg tgacagatgc cactagggga cggctggaga acatgctacc gttagcatgg
     2461 tttttgtcgg gaaacgctgt agaacggcgt cctgatgccg ttcggataag gttggcatag
     2521 ggatgccagc ctacgttccc gaatagaact agccgaaaag ccggctctcg agaggatgct
     2581 catgactact tcgcagcagt acgagtggac cgtcgtcaaa tcgcactggt gcccggccgc
     2641 caacgcggag gtggccctga tcgagaagcg ggtctacccc acggcagact tcctcggcac
     2701 cgagagcttc cgcgtggtcg agcaacgctg cacccacgac gtcgagtgca acctgtacga
     2761 ccacgtccac tgcaagtggg ctgggacgaa tccttacaac gatcctttcg ccgagagcca
     2821 gtaagacttc ggaagtctca agagactatc gaagtctgag ccttcgtgcg ccctttcagc
     2881 cattccctcg aggcggcgtg gtcaggtgag cgccgcctca cgcgtgtggg ccgccgaagt
     2941 ccccatcgtg ttacaattgc cgccaatgtc caccacctac acgtatgccg ccgacctggc
     3001 gcggcgacta ccggacgtcc cggccgacag cattatcagt cacacgctgt acagcggaga
     3061 cgatctgaag gcgacgctgt tcggctttgc ggcggggcag gaattgtccg agcacacggc
     3121 ctcgcagtcg gctattctgc acttcgtgga aggcgaagcc gatttgacgc tcggcgccga
     3181 cacgatgagc gcgcaggcag gcacgtgggt gcacatgccc gcgcaccttg cccacagtat
     3241 ccgcgccagg acacgcgtcg tcatgttgct gctgctcctc aagaccagta aatccgattt
     3301 ttgaagcaga gcgggctccc acgcccgcaa atcggcggca tgggagccgc ctccgctgac
     3361 aatgttggat ctactgagac cgggcaatag gatgccagga gcaaagtgat gaatacgacc
     3421 gggctgcgcg ggcacctgcc cttgctcggg gcagccatgc tggcgctgct cgccggacta
     3481 tgggctgggc tgatccggat cggctggcag tggccggcgt ggcagcccgc gttacctttc
     3541 ttgcacgggc cgctcatgat ctcgggcttt ctgggcacgc tgatcagtct cgagcgcgcg
     3601 gtggcgctct ccgcagcagc gcggcccgga gcacgccgag tctggacgta cctcgcgccg
     3661 ctgctgaccg gcctgggagg catcgtgctc ctgctcggct tgccgaacga aattggacgc
     3721 gcgttgatca cgctgggcag cttcgtgctg ctcgttgcat tcgggttgat cgtgcggctg
     3781 cgcccgtcgt gggacacggc gacgatggcc gccggcgcgc tgctatggtt ccttggaaac
     3841 ctgctgtggc tatcggggcg ctccacgggt aaggcggcgg tgtggtggat cggcttcctg
     3901 gtgctgacaa tcgccggcga acggctcgat ctggcccgcg tgctgctctt gcgccgccgc
     3961 gcgctgatca cgcacctcgc cggcatcgcc gtgttcctgg ccgggttggc gctcacgttg
     4021 tgggacttcc tgagcggagt gagggtaagc ggcgcgggcc taatcgcgat gggcgcctgg
     4081 cttctggctc acgacgtcgc ccggcgaacg atccggcaga gcgggttgac gcgtttcatc
     4141 gccgcctgcc tgctgcccgg ttacctatgg ctcctgttcg cgggcgggct gtggctgttc
     4201 ggcaacttca gcgcgggcca ggtgtacgat gcggcgctgc acagcgtgct gctcgggttc
     4261 gtgttctcga tgatcttcgg ccacgcgccg atcatcctac ccgccatcct gaatctgcct
     4321 ctcgcgttcc acgcgtcttt ctacgcgcat ctcctcctgc tgcacgtgtc gctcgtcatc
     4381 cggatcatcg gcgacgtggc cgcgtctccc tcgctgcgca tgtgggccgg cctgctcaac
     4441 gccatagcca ttctgctttt cttgttcaac acgatgcgca cattccgctc ggcacgctcg
     4501 cagcggccct cggcatcgac tacctgaatc tcccggcgaa acgatgccta ccctgacccg
     4561 ctggttcgtc aagtccgccc tgatctattt cgtcgccgcg ctcacgaccg gcgttgtgct
     4621 ggcggcgcgt cccgcattcg accttccggc ggcgctcgct gcgctcaacc cggtctactt
     4681 ccatctactg atggttggct ggatcacaca gctcatcttc ggcatcgtgc actggatgtt
     4741 cccgaagtat tcgcgcgaac agccgcgcgg cagcgaccga cttgcctggg ccacgtacgt
     4801 tttgctcaac atcgggttga tcctgcgtgc catcggcgag ccgatgcaag ccgcgcagcc
     4861 agaaggcgca tgggggtggc tgctggccat ctccgccatg ttgcagtggc tggccggcgt
     4921 ggccttcgtc gccaacacgt gggggcgcgt caagtgacgc gatccatccg ttggtccgca
     4981 gttgcactgc ggacgccggg cgacagtgca actgtcgccc caacatgtgt gtaatcacga
     5041 gattcgacgc cgttgacatg cctcgactga gccgtctggc gatccgcgct gcgctgctct
     5101 atcttctgct cggcttcacc ttcggcgggc tgatgctcct caacaaaggc atcccgctcg
     5161 cgccgtgggc ctggcgactc ttgcccgcgc acgtcgagtt cctgctgatc ggctggacgg
     5221 cgcagttcat cctgggagtc ggtttctgga tcttgccccg tttcagccgg ggacctgcgc
     5281 gcggaaacga gttgttcgcc tggctggcgt tcgggctggt caacgccggc gtgtgcctgg
     5341 cgggtctcgc tcccgtgctc aacgcgcctc cttcaatcgc ccttctcggc cggctcgccg
     5401 aagccggcgc agcgatcgcc ttcgccatgc atgtctggcc gcgcgtcaag ccgctggcgc
     5461 agtgaacaag cctcactttg aacgtggctc aagccgccgg ggcctgccct gagcagagtc
     5521 gaagggacgg cgcggcgagc cgggagacgc aggttccagg cgatcgagtg cgccgatcgc
     5581 gggtgtataa tggtcggcgt ggacgttctg gccgagattc ggcgtgctgt ccgtcgcttc
     5641 gatttactcc cgcgcggcga tacggtcgtc gtcggtgtgt ccggtgggcc tgattcgctg
     5701 tgcctgcttc acacactgaa cacgctgcgc ggcgaattcg gcgtcgagct gcacctggcg
     5761 cacctcaacc acgggctgcg tggcgcggaa gctgatgccg acgcgcagtt cgtcagcgcg
     5821 cttgccgctc agtggggact gctctgcacc gtcgcccggc gcgacgtggt cgccctcgcg
     5881 cgcgagcaca aactgtcgat cgaggaagcg gcgcgccacg cgcgttatgc gttcctcgcc
     5941 gaaacggcgc aacgcgtcga cagccacacc gttgccgtgg cccacaatgc cgacgatcag
     6001 gccgagagcg tattgatgca cttcttgcgc ggcagcggca cggcgggcct acgaggtatg
     6061 ctccatcaga gtgacctgct ggaattccga attctgaatt cagaattcag aattgagaat
     6121 tcagagttga aattgattcg tcccctgctc gccgtgccgc gcgcggacat agagcgctac
     6181 tgccgggagc acgatcttcg gccgcgcttc gacacgtcga atctcgacac gacgtttttc
     6241 cgcaatcgcc tgcgccacga gctgctgccg ctgctggaga catacaaccc gaacatccgc
     6301 caggcgctgc gccgcacggc ggacgtcgtc accgccgagg tcgaaatcct gcgcgacgcc
     6361 tccgagcaag cctggcgtgt cgcagcgatt tcagaaaccg gcgaggccgt gactttcaac
     6421 ctgagtgcct ggcgcgattt gcctccggcg atgcagcgcg ccacgctgcg cgaggcgatc
     6481 caccgcctgc ggccctcgct gcgcaatatc aacttcgtgc acgtcgacga cgccgtgaac
     6541 caggtgaacg ctgcgcggac cggcgcgcga gcgagccttc cccagcgtct gatgctcagc
     6601 gtcaactacg acgcgttcac cgtcgccgat tccggctacg agcctgacct gcctgactgg
     6661 ccgctgctcg aggtcgggca gatcgtgagt gtgacactat ccggtatcac tccgcttccc
     6721 gcctcggaat ggtcgctcga agccgtcgcg ctcgacaaat ggggcgacga ggtgttcgcc
     6781 aatcccgacc cgtggacggc ttatctcgac ggcgaagcgg ccggcttcga tgtgtcgatt
     6841 cggacacgcg cgccgggcga cgaattccac ccacagggca tgccctcgcc cgtgcgcttg
     6901 acgaactgga tgacgaacgt caagattccg cggcgcgcgc gcgatcggct gccgctcatc
     6961 gtcgccggcg gtcgcatcgc gtgggtggcc ggatgtcgcg tcggacagcc gttcaccgtc
     7021 agggccagaa cgcaaagcgt tcttcgactg tcattcgtcc gttgatcggc agcttgctta
     7081 tcgtgggcgg cgtggtatgc ttgtataaga gtatgcgacc acgagtgaac ctgtggatcg
     7141 aacacgacgg cgaggtcgtc ctcagcgact ggcgcgtgag cc
//
LOCUS       DBNHXB010000069        34291 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_13468, whole genome shotgun sequence.
ACCESSION   DBNHXB010000069 DBNHXB010000000
VERSION     DBNHXB010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 34291)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 34291)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34291
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_13468"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="AC162G_02560"
     CDS             complement(<1..331)
                     /locus_tag="AC162G_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074728.1"
                     /translation="MENQGRLGTARVELSKTSAVVKKALSYRYLIRYTLPNLGRGSAR
                     ELAFPLSRLGRQLFKEGYSAEMMKAIYLQDTQPYGLFDRIFLGYPLHRAIYHRLQILN
                     RLIETELR"
     gene            complement(454..1362)
                     /locus_tag="AC162G_02565"
     CDS             complement(454..1362)
                     /locus_tag="AC162G_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074729.1"
                     /translation="MSLQRHLTGVRTLKFEVWNFDMSFYVSIIGIDGSGKSTVTPALA
                     SLAAAELNVTAAAVGEGLSCKAPREDLCAPGFAPDGEMMSVRIGQWFRWISKSATAHR
                     RLYPLLKLLHLAAQARTVRQAVDRYQPGVIFSEGNLLLSAAGRAINYVDAHAAASALA
                     TLTNPFLRLEILYDYVMDGKPLSPDLIRAMPGLKMMRWLRRLDVRLKLGLMELPDAIV
                     YLDIAPEVALARLVASGQKLDPHENILDLTQARVMYRHVVSFFRRRRGERYARVVDVT
                     GLSVGQTLRHILDFARTLPIDRGAIP"
     gene            complement(1316..2425)
                     /locus_tag="AC162G_02570"
     CDS             complement(1316..2425)
                     /locus_tag="AC162G_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HIR5074730.1"
                     /translation="MSAQSVQRPGSAFLVVNKPSGNGHSAEDIEYLRAAFDDCFRWID
                     VRTFAVAEGHDEVVNLTRDYLAAVKGPCILLSGGGGGTNRALVQGFLKEVEAGTVGLD
                     DVLISSLRLGSGNLIPKHFGMPRDPLEGMKRISADLDARQARACCVYRCMLHYPDGMT
                     RQRYGLTMGGLGQFARVPDDIKRWRDAHRRLMRCASRLIPLEAINTIQYAAFSALRAV
                     RCIARPECAERVEIRHAHRRDRFRLFAGILLNFDFPQLPIRGGCDIGEPRLVLCCIPC
                     EGRRQTVRTLLDWRNLDRRIRKYEIIPGTPIEVEFVDHQQTTLALDEDTFTAPARIGF
                     EVAGLVRFVTGTSTGSAHRSFVPAAAFDRSPYFEV"
     gene            2804..3571
                     /locus_tag="AC162G_02575"
     CDS             2804..3571
                     /locus_tag="AC162G_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5074731.1"
                     /translation="MSEFVQWLTAEWEALEKREGKKYSARGLSIAAGLSPNTLYQLLK
                     HPEIKPRPETCQKLAVLFNVSPVWLLQLAGHVPSQGDAQPSPNLDVALQRGDLQHLVL
                     SAMDLSPGEVQLVQEVVDRLQAKRREADVASGAAVALVVEDTPDARATIVELLHAAGL
                     KVLEAADGQSAVDLMEASGALVDVVLMDYKMPRMNGVEATKRIREHYPDLPVLFVSAW
                     DEPEMKRAAFEAGAAEYLVAPVGYDELVEAVARVRRR"
     gene            complement(3674..4537)
                     /locus_tag="AC162G_02580"
     CDS             complement(3674..4537)
                     /locus_tag="AC162G_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074732.1"
                     /translation="MPRSLTSAPTVTPLRLTPRVLDGDLLNQITQLEAQMPRANSQGF
                     VVPADAEKTAFAEIVKNLEAGEIDRAGGRAAENDYELVRYTDRGDAHAISWLLRESKP
                     IRKGWGLYAIRQNAAQDVIVEAPHPLADEETPAIAARLYRALQARALLIAGAHRNANV
                     DGTADAAHDAQTIFEALHESLTKSDSTVIVQIHGFAADKHPGYPQMVLGHDEAKADGV
                     LEKLADTLRRKGVRVGICDGRSWTELCGEENIQSRHNGPAIFIHLELAESVRAKPDVL
                     IAAFKEVLTGH"
     gene            complement(4654..5658)
                     /locus_tag="AC162G_02585"
     CDS             complement(4654..5658)
                     /locus_tag="AC162G_02585"
                     /inference="COORDINATES: protein motif:HMM:NF025467.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein
                     PgsC/CapC"
                     /protein_id="HIR5074733.1"
                     /translation="MHNYGLPTEDVRLALALGVLLSILAYERWRVTGGAAVIAGYLSM
                     FIGRPLYIVTTVLLAIVTYYVVHHFVARKMFLYGRRRLVMMVLVGMAFQFTIGFIAFF
                     GSADAPWLVGLYGVGFVLPGLIAHDMERQSMRLTVLTVLGTSFVTYLLLQSFLAIKNN
                     LPIYWNSIAFEDKTIVYSYQTQLLIPAVIFSVIVSALMFEWAGIRSGGFLTAGYAALF
                     VLRPQHLLVVGLIGVATYYFVTRILIRHTPIFGRSKFAMMVLTGLVFTWSAEVALSAL
                     TRNVFVLFAGFSVISPMIAALIANDSERQGLPKTLLGAVVATLIVFMAIKGIDLLLIT
                     "
     gene            complement(5716..6993)
                     /gene="pgsB"
                     /locus_tag="AC162G_02590"
     CDS             complement(5716..6993)
                     /gene="pgsB"
                     /locus_tag="AC162G_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HIR5074734.1"
                     /translation="MFELGLTLIIYLVFIAYWRRRQAAHEKRLPHYKVRVHVNGIRGK
                     STVTRLIAGVLREAGYRTLAKTTGSAAVTINFDGEEVPIPRRGAANVREQQDIIARWE
                     SQQAEALVIECMALQPKFQEWTENMVIRSHIGVITNVREDHQDVMGETLPEIAVSLAH
                     MCPTGGYLVTAEYDPDLQVVLRREAEKRNSQLVVADPIRVRDDDIAQFDYLSFKDNVA
                     IGLAVADLLGIDRDVAMRGMIKAKGDIGVVRLQRVTLRGKPVIWANLFAVNDRESMII
                     SLDMLEAYCDDKTVRIGILNNRYDRERRAKQFGDVAAKDLKFDWLITFGAYEDLVTRK
                     LLSNNFPRERIVNLGFSVSPTIEQIIDAIAGMIPEGWQAMLVGFVNIHTPQAEMLMEY
                     FEHLKDTYAVTPTELEGDIGYLRRRRFEMAHVG"
     gene            complement(6999..7847)
                     /locus_tag="AC162G_02595"
     CDS             complement(6999..7847)
                     /locus_tag="AC162G_02595"
                     /inference="COORDINATES: protein motif:HMM:NF037653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074735.1"
                     /translation="MKMTKAARTRGWAVMLALLTIVATVLIVMVNPLEAAMVYLALLF
                     GTIALGLLTGPWGGLVAAAAGVVTITLLNQYAGIYARENAVAIIAAELAAFLLIGPLA
                     GRLGRSISRVQRKADRWLERAEELSVYDESFGTLKPAWALTRLDEEVMRAQRFQRPLS
                     IALLQLQANPDVPTGQHTHRIAALQAVMRVARAATQPPTLITHAGDDQVLMILPEQDT
                     TQAQSVIAALQQRFEREVYFPPTGDQALGKPIREWGQLHVSLVSLNGTSETGETLLAK
                     ARAAQE"
     gene            complement(7834..9879)
                     /locus_tag="AC162G_02600"
     CDS             complement(7834..9879)
                     /locus_tag="AC162G_02600"
                     /inference="COORDINATES: protein motif:HMM:NF015149.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HIR5074736.1"
                     /translation="MVRKPIRLCFVLLLLWPSGVLAQGAEPLRFTLADLGEKDIVVNV
                     IFDQATLHFPLPEGKRIERATLKLHLAHDEQLLPDRSGVTIALNGEPAINLPLTTDNL
                     STTLEIPLPIAALRPGDNQLLFRFNQRLQATGCGDLGDNNLWTQIFADTAIELEGQDV
                     PPSLDLARYPAPFTTLSTLAGSPHISIVLPERPTSAELTAAAQIAAALGQAAEWKEPP
                     VRAATIDQLDEARTDDHLIAIDTARRNPLAQQASAGVTLQTSPRNPQRVMLIVSGTDA
                     DELSRAVALLTTQSARAQLGGTHVNPTEFTAQPVPQRPALVTFAAAGFADRQVNGIGL
                     HDLYYPIDVPYDWKITNDATIELRFAHARGLADESNMRASINGLRVAVVHLTDRNADD
                     GRLTIQVSPRKVRPGRNWLHLKFDLRLPNQDCNFRYLDQAWASISAASSMLNLAHVNS
                     EPPLDLRYWPSPLTTPDDLSASLFVLSAQPTASDLSALVVLAAKLGTFSMADGLRPRA
                     SMDDAWREDVRPGDHAIAIGLPETNKVLQQYDAQLPQPLTRSNGVIQPSSGRELHPDE
                     QTNSAGYVQLLNAPWSAGNTLITISAPDETIMQRTLASLPVLGQLLQRHGNVAIVNSQ
                     GLTGLTLGGWEGALSPAARRVMAPIMLGLVVVVAVAGVWLSRRRTAPRQQESEHEDD"
     gene            complement(9888..12083)
                     /locus_tag="AC162G_02605"
     CDS             complement(9888..12083)
                     /locus_tag="AC162G_02605"
                     /inference="COORDINATES: protein motif:HMM:NF024299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5074737.1"
                     /translation="MNSSCRRVLPEARRCWLLLVVWLAAACTGALAAPNARSYIDAGQ
                     QALRDRDWRAALESFNAALQIEPDRHEAWTGRGWANLNLQSAEAARADFDAALKFGTT
                     DASTWAGRGRARLKLGDNAGAIDDLTQALQRSPLRAGSYFDRGLAYYRLRQYEAAIQD
                     FTRGIRSDARSVRGYYDRGLAYLKARQYDQALSDFNQAIVISPTYAAVYVQRAKTYDA
                     QGHYPQAQADFDRALALQPDDAATYTARGYTFYHSQQYEKAAQDFDQAIRLNTTDANA
                     YYGRALAYIHQQQPDRALPDLDRALQLDAQRPAPYRERGRIYYARRDFTRALADFEQA
                     LQLNPDDDSARYWRGLALYRLGQYARAAPDFESVAKGSSTHALSARYYAGLVAYHLQD
                     YAQAAQDITAALADTTPTDTQRYQRGLALYRAGDYESAAADFAAVAQGTSPYTAKARY
                     YAGLTAYHLQDYAMAASHFSTAFQSLTPTAAQLYQRGLAQYHLKQYAPAFADFDAAIK
                     SGRTTTLTTYYRGLAAYHLRDYAQASADFSAALKAGSAPANTVFQRALAEYHLGQPDL
                     AQRDFDQALRIDPRNASAYFYRSLIARDAGRVNDALDDLTQALAIKPSDPAALYARGR
                     LYYQLPDFVKAQADLNRLVQIDPRHAGAHYLLGLMLYKGGAFDQALHNLSQAIALDPA
                     RVYAYYYRALTHRKLDHTVEARADLMQVLELAPQTHLADLAWRALRKLP"
     gene            complement(12183..12299)
                     /locus_tag="AC162G_02610"
     CDS             complement(12183..12299)
                     /locus_tag="AC162G_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014453.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR5074738.1"
                     /translation="MTRVARLLRRLSLDALPQVLNVLIGDLKTLSNRFLAAI"
     gene            12977..16417
                     /locus_tag="AC162G_02615"
     CDS             12977..16417
                     /locus_tag="AC162G_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5074739.1"
                     /translation="MSNLSRRQFLITGGVTTAGTLLGAKLGLRFLQPIVDVPNPLAAY
                     PSRDWEQVYRDQYRYDSTFTFVCSPNDTHACRLRAFVRNGVMMRAEQNYDVARYADLY
                     GNTATPHWHPRGCKKGQTFHRRVYGPYRLKGPLMRRGWQEWADAGFPYLDAANKSKFK
                     FDARGQDEFLPVDWDTAYDYIARGMVAIAKQYSGAEGARLLAREGYPPEMIEAMGGAG
                     TRTFKCRGGMGLLGVIGKYGMYRFSNTLALLDVHVRGVNPEEALGGRNWSNYTWHGDQ
                     APGHPFVHGLQASDEDFNDLRNSLLHIQCGKNLVENKMPDSHWFVEAMERGAKIVTIT
                     PEYSPPATKSDYWIPIRPQTDTALFLGVTRLMIDNGWYDVDFVKRFTDFPMLVRADTL
                     KRLRAADLFPDYQLQLTADSASFALQGLTPEQYEILGDYVVIDAATGQPTAITRDDVG
                     DKMAEKGIDPRLDYRGALTLADGSQVEVLTLWSMYVDHLKDYGLDAVAEMTHAPKDLI
                     ERLAQDIATTKPTAIHIGEGINHWFHATLANRAQYLPLMLTGNIGLPGAGCYTWAGNY
                     KAALFQGSPWSGPGFKGWVAEDPFQPSLDPNASGKDVHAHAFTQDEEPAYWNHSERPL
                     IVDTPKYGRKVFTGTTHMPTPTKAMFFTNVNLINNAKHAYEMIKNVDPNVPLIISVDI
                     EMTATIEYCDFALAANSWVEFQNYEVTASCSNPFLQIWKGSLPPLYDTRDDAVILAQL
                     AAKLGEVTGDPRFADYWKFALENRAEVYIQRLLDSSTTTAGYRFEDIMAGQYGEPGAA
                     LMLFRTYPRIAFWEQVHDNVPFYTDTGRLNAYCDIPEAIEYGENLIVHREGPEATPYL
                     PNVIVASTDFIRPDDYGIPLDAMGWEERTVRNVKMAWADAKQTKNPLWEGGYRFYCVT
                     PKTRHRVHSSWSTVDWSNIWDSNFGDPYRVDKRLPHVGDHQLHIHPQAARDLGLEDGD
                     YVYVDANPADRPYIGWKPDDPFYRVARLMLRVKYNPAYPYHIVMMKHAPFIATERSVK
                     AHETRPDGLARSEGTGYQADLRYGSQQSVTRDWSMPMHQTDTLFHKAKVATAFIFGGE
                     ADNHAINTVPKETLVKISKAEDGGLGGQGVWEPATTGYSPGNESDFMRRYLSGDLVET
                     "
     gene            16435..16629
                     /locus_tag="AC162G_02620"
     CDS             16435..16629
                     /locus_tag="AC162G_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074740.1"
                     /translation="MTDTSLPAQCINCERSVGEVPLIPIVHRDGPAHICPQCLPILIH
                     QPDALTGKLAGVESLQPHEH"
     gene            16638..16904
                     /locus_tag="AC162G_02625"
     CDS             16638..16904
                     /locus_tag="AC162G_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074741.1"
                     /translation="MPKDEFEHDDPMELVGMILPGDEATTEAMAETFVEEYVRMGWDD
                     ARLMKLFTHPFYVATHAVYRLKGEAYVRELIRKTCAKWQRIGSG"
     gene            16997..18259
                     /locus_tag="AC162G_02630"
     CDS             16997..18259
                     /locus_tag="AC162G_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5074742.1"
                     /translation="MAEVYNWQLGRAMAYPFAEAHPQKQFVAVFNINRCIGCQTCTGA
                     CKATWTFSKGQEYMWWNNVETKPYGGYPHHWDVKLLNLLDQAHQTAGKKMSWSAPRRD
                     EAAAPYGVYDGMTIFEAADPNNNVLGYLPTDGEWRAPNIFEDVSPAYRGDDLGISIEG
                     ASLPEHKTWFFYLQRICNHCTYPGCLAACPRKAIYKRPEDGIVLIDQERCRGYRKCVE
                     GCPYKKAMFRGTTRTSEKCVACYPRVEGSDPLTEGVPMETRCVAVCPGKIRLQGLVDI
                     AEDGAWVENRQNPLYFLVKVEQVALPLYPQFGTEPNVYYIPPRWVNRSFLRQMFGPGV
                     DDAIERYTAPSRELLAVLQLFRTTQKIIFRYEIEEGPLVHETEVNGKPWSMYNDTVIG
                     FDADDKEAVRLSVVEPIHERPPERQNSI"
     gene            18325..18693
                     /locus_tag="AC162G_02635"
     CDS             18325..18693
                     /locus_tag="AC162G_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5074743.1"
                     /translation="MGSEHRDPQVFRILCGDAVETRHTPYGSVGTVFSGEGIEAVWVS
                     KQDEAIEPGWFSQSTVDLILVVQGQLHVEFERPDLAPRVLEPGNLLVLPANTRCRAYR
                     WPRDRKEATVFLAVYPVKQE"
     gene            18706..19464
                     /locus_tag="AC162G_02640"
     CDS             18706..19464
                     /locus_tag="AC162G_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorD/DmsD family molecular chaperone"
                     /protein_id="HIR5074744.1"
                     /translation="MKSSTVEATTEETFGRAQAYRLFADALVYPDEAFAERLADGYAA
                     DLLECLTMLDASCDMAWSEPFERLVRALAGATLADVQRAHRLAFGVAAGTPPYETEYG
                     QPHAFRQSQELADIVGFYRAFGFDVGGGVRERPDYLGVELEFMYLLACKQAYAVAGGL
                     ADAAEVCHDAQRKFLRDHLARWIGMYARRLNDQAQGRNAYAVLADLAAAFVGEDARRL
                     GIERDSMAPGDARPTPPALSLSCMGCTEEDEFSF"
     gene            19448..20371
                     /locus_tag="AC162G_02645"
     CDS             19448..20371
                     /locus_tag="AC162G_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074745.1"
                     /translation="MNFHFDPRLMEEAVGAALARRPMLAARYRAGVEALYVDVPLGER
                     EPAFARLHAKWFESLELDRPLRAIFEEFPVVVAAARDVIVARADRRRDEGAVLAPRTT
                     RLGLRLTPARFTNDDALSCLMRHELTHVGDMLDPEFGYPDRPPSGATAGENLIRDRYR
                     LLWDLSVDGRLARAGKKSIADAAMRRRELDALYCSLPEATRSAIFDRLWGATDLTHDR
                     LWAVASDVRALVAFAGVALAAGASNGPLPGARCPLCRFPTYVWSPPLEPKVVSIVRLD
                     FPRWQPDEGVCERCAELYRMRVGDWELEIGV"
     gene            20517..21566
                     /locus_tag="AC162G_02650"
     CDS             20517..21566
                     /locus_tag="AC162G_02650"
                     /inference="COORDINATES: protein motif:HMM:NF021010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HIR5074746.1"
                     /translation="MLNRTLHLRNWTRALLPIALLLALAATALRVPTASSQGVTIVAR
                     QVEADLPVDDPDSPVWEQAVPVEVPLAAQTAAVPRWPNPGVQAVTVRALHDGSRVAFR
                     VEWADATKDEHMLRVDQFRDAVAIQFALAKGQPFFCMGQVGGLVNIWQWKADWEADLA
                     GYQDMESVYPNMAADDYPLTEAEPGEIATVYEYDPTYLAALAAGNLFASPQRISSVED
                     LTAGGFGTLTAQGRAGQNVHGKGVWRDGKWRVVFDRALESDEADDMTFELGQVASIAF
                     AAWDGSNGERNGQKATSNWYSFQLEGEAPAPPAQWAPPGAPELVQMLAFVAGIGLAIW
                     IMAAVVDRMGRSRGV"
     gene            21569..22507
                     /locus_tag="AC162G_02655"
     CDS             21569..22507
                     /locus_tag="AC162G_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074747.1"
                     /translation="MRWQNWLLAAALADWLIGRTLSRAAIFMPKSGIMVTVTEGMMTS
                     GQIAFNLAAILTLAAIVWLAVRGLRHVEHNLPLSAALSGLVGLSIAGVFVPPGPVTLI
                     VFQMLTLAALTILVVESRRAQPDCSARWAVVPPVLAIASITSFKMVQTVAQLTDAPAP
                     ASLALLAFQVGELFVVITPPAVWWAYGKTGRVRDWVIAGIPAALFTIAYLANPSMTAT
                     MATWSVGLSLYLPWPVYAISLWLAAVTVLRALRAGQGAGYGLAYLTAAGYSMQLSSQV
                     MLGLVGIWMLGLTSLSVGRFVSPPLRANKPLQSIIR"
     gene            22948..24471
                     /locus_tag="AC162G_02660"
     CDS             22948..24471
                     /locus_tag="AC162G_02660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR5074748.1"
                     /translation="MYSHPTAIVIGAGLGGIVAAARLARAGYQVTVLEKNATPGGRCG
                     QLIRAGHRFDIGATLFLMPEVFGQTYAELGERMEDHLDLRRIDPTYRIHFADDTTLAL
                     TNNLNDMQAQLEAIEPGSFGGLLRYLVEGERHYHVSLERFVGRNFYNLLDYFSLQNLP
                     LLFQLKALTRHYDNVGHYFQHPQLKAAFTFQNMYLGLSPFDAPATYSLLQYTELADGV
                     WFPMGGLYRIIESLASIAQAHGVEFWYRSPVKQIEVVGNRATGVVLQNGARLDADVIV
                     ANADLPYVYRDLLPDQDEADRLERLKYTCSAIMFYWGTDQVYPQLGHHNIFLAGDYRG
                     SFDRIFQDHTLPDEPSFYVHAPARTDPAAAPAGHDTLFVLVPVGHLDESRPQDWDALK
                     AKARSTVLDRLSRLGLSDLEQHLKFEVSTTPVNWMNRFNLAKGAAFGLSHNFWQVGYL
                     RPQNRHRRYRNLYFAGSSTHPGTGLPMVLLSARLTTERILSESGQPRPVMMINPLPA"
     gene            24537..25478
                     /locus_tag="AC162G_02665"
     CDS             24537..25478
                     /locus_tag="AC162G_02665"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HIR5074749.1"
                     /translation="MITTLPSPTWERALLSLAYERRSTEAAVPKPVAERKLLERAYAR
                     CESITAAHSRSFYLASGLLPADKRRAVRALYAFCRVSDDIVDCPGGDAAATLAAWRQH
                     ALAAEPPASDLVALAWADTRLRYRIPIRYVEQFLDGVARDVRQKRYRTFEELAEYAYG
                     VASTVGLMSMHIIGFAGREALPYAIKLGVALQLTNILRDVGEDWRAGRLYLPAEELAR
                     FGLAESDVAAGRVDDRWREFMRYQIERNRRLYAEAWPGIKLLHPDGRFAIATAGELYR
                     AILADIEAHDYDVFTRRAHVSGPRKLLNLPGIWWRNR"
     gene            25528..26724
                     /locus_tag="AC162G_02670"
     CDS             25528..26724
                     /locus_tag="AC162G_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HIR5074750.1"
                     /translation="MKHYDFVIVGGGLAGLSLACHLIDSPLRERSILIVDKDAKDKND
                     RTWSFWTRWPTLFDSIVHRSWNRLHLAGENFDQIIDLGDYRYQMIRGIDFYRFARQTL
                     SAHPNVEFLQARVEQIEDDAGEARIVANGQPFAGKWVFDSRFKPPELKPDPTRYHTLN
                     LHFKGWEIEAPEPAFNPQAATFLDFRTPQKGDTRFFYVLPLSKQRALVEYTLFSAELL
                     KRDEYERALKDYLENVLQVGDYRVLSEEKGIIPITDQPFPRRAGRHVMTIGAKGGRIK
                     PTTGFAFLRIQQDSAAIVDSLLRTGQPFDIPADSARYRLYDSLMLQIMLRRGAQVQQL
                     FTLLFKNNPIERVLRFLDEAGSVWENLQLIPTLPPAPFLEALFRLKVLRQMSPNSIDI
                     RDARTA"
     gene            27132..27623
                     /locus_tag="AC162G_02675"
     CDS             27132..27623
                     /locus_tag="AC162G_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR5074751.1"
                     /translation="MSKIDTLVQDFLAQKKIAVVGVSDKRETGCNLGYRKFKAAGYAV
                     SAVNPRLTTFEGDPCYPDLKSIPDKPEAVFILANPKVTEQIVQQCVDLGIKHVWMHCL
                     MGTKPGLGAGTTSVSQDAVGMCRENGITVIPGACPNQFLKPDFGHALMRVMFRTLGFH
                     SVN"
     gene            27828..28289
                     /locus_tag="AC162G_02680"
     CDS             27828..28289
                     /locus_tag="AC162G_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074752.1"
                     /translation="MSQILIALSMWLHALGTVILIGHYLLLSLIYLPVLAKDRGTILS
                     EISKHSRTWLYLSLLIFIITGVYLTLADPNYLGLANFGNLWGILMLVKHVLILGMIAM
                     GFWFNAILRVGPMMSSNSGAAQAAIRMRLYANLMAISGVLVLFLTALAQVQ"
     gene            28344..29873
                     /locus_tag="AC162G_02685"
     CDS             28344..29873
                     /locus_tag="AC162G_02685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR5074753.1"
                     /translation="MNIKRFLFPSSLVGAVLALILRAFARVFAKPASAKPVSNSSSYD
                     AIDAYVEEQMHRLKMPGVSLAIVEGDRIVHLRGFGRARPGGEPPLPHTPFPIGSLTKS
                     FTALAVMQLVEAGKIDLDAPVQRYLPWFRVADPQASAQMTVRHLLNQTSGLPTSSGEP
                     CPADFDTSPGATERQARVLSTLVLTRPVGSAFEYSNSNYNLLGLIIEAVSGELYADYV
                     QDHIFTPLGMSHSYTTKAEAKQNGLAVGHQYWFGIPFAAPNMPLPHRGLAGGGLISCA
                     EDMARYLIAHLNGGRYGDVQILSAAGIDELHRGVADFRAMGLSLGQYGMGWFVDKIGQ
                     TKLVFHGGTLPDFASHMALLPEHKKGVVLLFNACQHWMNPVLADLGNGVAALLAGEQP
                     AHLPFFYVVPWALRGQLLIPALQIAGVAATLRLLRRWRLNPERRPSGGRKWGLHILLP
                     LIPNLLVALTLRPLLGKRRGYLMLYMPDYSWIAMICGSFSLVWSFLRTGLVLQALRRS
                     S"
     gene            29973..30818
                     /locus_tag="AC162G_02690"
     CDS             29973..30818
                     /locus_tag="AC162G_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIR5074754.1"
                     /translation="MNNNIGPYHVVDLTPARRVWLNTLELSWSTHCMYGLLEVDVTVA
                     RRFIAEHKARTGETLSFTGFLVSCLARAVDEDKAVQAYLKGRKQLVLFDDVNVGLMVE
                     GKIGEKRALMGHVIRGANHKTYREIHQEIRSVQSEPVPPSRGMPTWFRSAMLLPWPLS
                     RLIKALLGMAMRRDPTIPVSMAGTVGITSVGMFGGSHSGWGLTPTSQSLALVVGGTAW
                     KPAVVAGRIEPREILNLTVAFDHDIVDGAPAARFTRRLVELIESGYGLDEIEPANHQY
                     VSNPA"
     gene            complement(30980..31948)
                     /locus_tag="AC162G_02695"
     CDS             complement(30980..31948)
                     /locus_tag="AC162G_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR5074755.1"
                     /translation="MLFTPDSITLDGIIPPIRHAGGKGSGLYWLQVNGFRTPPTWVLS
                     TTVFDRMVEHAGVAAVVREIDQATASTPDWESAQRMLDDVEPQRQAIVNALEESPLPE
                     AVLALLLRLPTLDHPQWAVRSSATVEDRDAHSYAGQFFSMLSVPSDLASLTQAIRQVW
                     ASAFKREVLSYRAHSGTTWPKMAVILQPMKPITAQERSGVAFSHSPVPNLPGVLIQAM
                     FGAGRGVVEGHGGDLYAMQDVNVTERHMPPPHITISGAAGGEIRAPTPSSPVFTADEV
                     QRLASYVRTMAEYWGRPINVEFIWYAEQAEPLFVQIRTDTSRLAPA"
     gene            complement(31963..33603)
                     /locus_tag="AC162G_02700"
     CDS             complement(31963..33603)
                     /locus_tag="AC162G_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012609.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HIR5074756.1"
                     /translation="MTELLLSGHNTQETTGLARPATATMTEKGLDAQFDFVLEQLGGD
                     LSRFDRTVKATQSVQGRLYLNMEYFARTISKVVPFDTSSIGVPPEMVKNLSRPSLGQQ
                     MVLLPRLRNVYRWATHFYRHTLPGVDHDLHRLYWQLRTGADLAPAWALFEPGLYKRSR
                     GTDRAHVVISLIVVSVDTILRQRVPGLLGLFAGQSTATSQIGRHIWALRETAEACGEQ
                     VCQMLRDGVLDLERYRARPEAAPLVAGIENFLIVYGHRGFRHEADFETERLADHPEHI
                     LLAIGGQLAESESPDARAEAARQIALDNLNRMNPLSRAVWRRVLRWGQQLIAWREASK
                     SNLALRQAAYGLAARRLARYFFPDESDDILMFYSMDEFLAFVLARGEKRVKRETLDAR
                     RAQYELYSSQLPPPELIWYDQDTGRWTPVQERERAALKPFVTHREGIPASAGSGPAEG
                     IAIVTNDPLDAGQRLLRLRGPVVLVTRLTDPAWSSLFRRLSAVVTELGGVISHAAIVA
                     RENGLPAVVGLPEATRWVRDGQRLRVDGATGAVDIVDT"
     gene            complement(33600..>34291)
                     /locus_tag="AC162G_02705"
     CDS             complement(33600..>34291)
                     /locus_tag="AC162G_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074757.1"
                     /translation="PLSGGPTPSHDASGPTVKLACLEPWAKWSCIFDGPLTDIASSHS
                     ASFALDFQFHADGPPTRYQFGPYQQVQQDGVMSGSFRLDGQRYGGEFLCSRDRSWGRR
                     QMHIAEDWIVTSLPRSMYLAVIHIDGQQHHFGHFFLPDGQREAFRAPAVSADGNGWRI
                     ADDQTDAGVWQVERVTSPFVVHLGPPGQEAVRPEAHAGDLYRDEIGPAMYTAPDGRRI
                     PGFLERARRVG"
BASE COUNT         6520 a        10514 c        10731 g         6526 t
ORIGIN      
        1 ggcgcagctc cgtctcgatc aggcggttca gaatctgcaa gcggtgatag atggcccggt
       61 ggagcggata acccaggaat atccggtcga atagaccata gggctgagtg tcctggagat
      121 agatggcttt catcatctcg gccgaatatc cctctttgaa tagttgtctc cccagtcgtg
      181 acagagggaa cgccagctcg cgggcgctcc cgcggcccag gttgggcagg gtataacgta
      241 tcaggtagcg gtaacttaac gccttcttga cgactgcgga cgttttcgac agctcgacgc
      301 gggccgttcc cagccggccc tggttttcca tgtgtccctc cagattactc agtccgctcg
      361 tcgttgacgc atccccaacc cgtggttgat gcatccccat gcgtcaacag cgggcatagg
      421 gatgcccgct ctacgggctg taatatgcgc tcgtcagggg atagctcccc tgtcaatagg
      481 cagcgttcga gcgaaatcta gaatgtgtcg cagggtctgt ccgacggaca gacctgtcac
      541 gtcgacaacc cgtgcgtatc tttcaccccg gcggcgacgg aagaacgata ccacgtgccg
      601 gtacatcact cgagcctggg tcagatccag tatattttcg tgaggatcga gtttctgccc
      661 gctcgctacc agacgagcca gcgccacttc cggagcgatg tccagataga cgatcgcatc
      721 gggaagttcc atcaagccta atttcaaccg gacgtcgagc cggcgcagcc agcgcatcat
      781 cttcaggccg ggcatcgcgc ggatcaggtc gggcgacaac ggcttgccgt ccatgacata
      841 gtcgtacagg atttcgaggc ggaggaaggg attcgtcaag gtggcgaggg cggacgcagc
      901 ggcatgcgca tccacgtagt tgatcgcgcg accagcggct gagagaagca ggtttccttc
      961 ggagaaaatc acgccgggct ggtaacggtc gactgcctgc cggacggtcc gcgcctgtgc
     1021 agcgagatgg agtagcttca ataacggata cagcctgcga tgcgccgtgg ctgatttcga
     1081 gatccaccgg aaccattgac cgatccggac ggacatcatc tccccatcgg gcgcaaaacc
     1141 cggtgcgcat aagtcttctc gcggggcctt gcatgacaga ccctcgccga cggccgcagc
     1201 cgtcacattc agctcggctg ccgcgagact ggccagggct ggcgtcaccg tgctctttcc
     1261 gctgccgtcg ataccgatga tactgacgta gaagctcata tcgaaattcc aaacttcaaa
     1321 cttcaaagta cggactcctg tcaaatgccg ctgcagggac aaacgagcga tgcgccgatc
     1381 ccgtggaagt gcctgtgacg aatctgacaa ggccggcgac ttcgaatcca atccgggctg
     1441 gcgcggtgaa tgtgtcttca tccagcgcga gggtggtctg ctgatggtct acgaactcca
     1501 cctcgatggg tgtgccggga ataatctcgt acttgcggat acggcgatcg agattccgcc
     1561 aatccaggag cgtgcggacg gtctgcctgc ggccctcaca cgggatacag cataagacca
     1621 ggcgcggctc gccgatgtcg catcctccgc gaatcggaag ctgggggaag tcgaagttca
     1681 ggagtatccc ggcgaacagc cggaagcggt cgcgccggtg agcgtgcctg atttcgacgc
     1741 gctcggcgca ctcgggccgg gcgatacacc ggacagctcg cagcgcactg aatgcggcgt
     1801 actgaatggt gttgatcgct tcgagcggga tcaggcgaga cgcgcagcgc atcagccggc
     1861 gatgagcatc cctccagcgc ttgatgtcgt ccggaacgcg cgcgaactgt cccagcccgc
     1921 ccatcgtcag cccgtagcgc tgcctggtca tgccatcggg gtagtggagc atgcagcgat
     1981 agacgcagca ggcgcgggct tgcctggcat cgagatcggc ggagattcgc ttcatccctt
     2041 cgagcgggtc gcgcggcatg ccgaaatgct ttgggatcag attgcccgaa cccagccgca
     2101 gcgagctgat caggacgtcg tccaagccga ccgttcccgc ttcgacttcc ttcaagaagc
     2161 cttgaacgag cgcccggttc gtcccacctc cgccgcctga gagcagaata cacggacctt
     2221 tgacggctgc aagatagtca cgcgtgagat tcacgacttc gtcgtgtccc tcggcaacgg
     2281 cgaacgtccg aacgtcgatc cagcgaaagc agtcgtcaaa tgcagctcgc aggtactcga
     2341 tgtcttccgc cgagtggccg tttccagagg gcttgttcac gacgaggaag gctgacccag
     2401 gtctttgaac tgattgtgcg ctcatggttg tccacctctt cattaagatt ggctgcttcg
     2461 tgcttttcag attgttaatt ataatgtgga ttaactaatc gtaattatca tttattatag
     2521 tctgaatatt ctgccggtga agtagcaaaa tggtactata tgacgatccg tagcttgcgc
     2581 ttttgtgagg tgcgcattat gatgtgcaca agatgatgtg cacacgagga tgtgcatcga
     2641 ttcatttcat ttaaccgctc tgactctaca atagaccact ttgcaggcac gcactatgaa
     2701 ctgcgtcaca tgctcacaag acctggcagg tctttagggc gcggagcgtg cctgcaactc
     2761 gcatcggcgg gcaagacttg ccaggtctat tcggaggtga attatgtctg agttcgtgca
     2821 gtggctgaca gcggagtggg aggcgctcga aaagcgagaa ggaaagaagt acagcgcccg
     2881 ggggctgtcg atcgcggctg gccttagccc gaatacgctg taccagttgc tgaagcatcc
     2941 ggaaatcaag cccaggccgg aaacctgtca gaaactggcc gtattattca acgtgtcgcc
     3001 ggtgtggctg ctgcaactgg cggggcacgt gccttctcaa ggggacgcgc agccgtctcc
     3061 caacctggac gtggctcttc agaggggtga cctgcaacac ctcgtcctga gcgccatgga
     3121 cctgtcgccg ggcgaagttc aactcgttca ggaggtggtc gaccgactcc aggcaaaacg
     3181 gcgcgaggcg gacgtcgcca gcggagcggc cgtggcgctg gtggtcgagg atacgccgga
     3241 cgcgcgcgct acgattgtcg aattgctgca cgcagcgggt ctcaaagttc tggaggcagc
     3301 cgacggccag agcgcagtgg acttgatgga agctagcggg gcgctcgtgg atgtcgtcct
     3361 catggactac aagatgccgc gcatgaatgg cgtcgaggcg acgaaacgca tccgcgagca
     3421 ctatcccgac ctgccggtgc ttttcgtctc ggcctgggac gagccggaga tgaagcgagc
     3481 cgcgttcgag gcaggcgcgg cggagtatct ggtggcgccg gtcggatatg atgaactcgt
     3541 cgaggcggtc gcgcgggtgc gacggcggtg agaagatgat cgacggcgcg cggcgggcga
     3601 atcgaggtga gcagcaagca gatcggtttg gcgcagcctt tgaggccggg ttgatcgatc
     3661 ggttattgga cggctagtgt ccggtcagca cctctttgaa cgcagcgatc aaaacatccg
     3721 gcttggcacg caccgactca gccaattcca ggtggatgaa gatcgccggg ccgttgtgcc
     3781 gagattgaat gttctcctca ccgcataact ccgtccacga tcgtccatcg cagattccca
     3841 cgcgcacgcc tttgcgccgc agcgtgtctg ccagcttctc cagcacgcca tccgctttgg
     3901 cttcgtcgtg gcccaagacc atttgtggat aacctgggtg tttatcagcc gcaaagccat
     3961 gtatctgaac gatcacggtt gagtctgatt tcgtcagcga ttcgtgcagc gcttcgaaga
     4021 tcgtttgagc gtcatgcgca gcgtccgctg tgccgtcgac attggcattg cggtgcgctc
     4081 ccgcgatgag caaggcgcgg gcttgcagcg cccgatagag ccgggccgcg atcgcgggcg
     4141 tttcctcgtc agccagcgga tgcggcgctt cgacgatcac atcctgcgcg gcgttctggc
     4201 ggatggcata aagcccccag cctttgcgga tgggtttcga ctcgcgcagc agccagctga
     4261 tcgcatgggc atcgccgcga tcggtgtacc gcaccagttc ataatcgttt tcggcggctc
     4321 gtccgccggc tcgatcgatt tcgcccgcct caaggttctt cacgatttcg gcgaaggcgg
     4381 ttttctcggc gtcggcaggg acgacgaaac cctggctgtt ggcgcgcggc atctgtgctt
     4441 cgagttgagt gatctgattg agcagatcgc cgtcgagcac gcgtggggtc aggcgcagcg
     4501 gcgtgaccgt aggcgcggac gttagtgaac gaggcacggt cggcttaggg tgtggcgatg
     4561 cgatcggtgc tgccgaagtg cgagaatctg tgctcgacgc gtcacagcct gccaggagca
     4621 agacgatcaa ccaggcgctg agggaacgca ccatcaggtg atcagcagca ggtcaatgcc
     4681 cttgatggcc ataaacacga tcagcgtggc gaccactgcg ccgagcagcg tcttgggtaa
     4741 accctgccgc tcgctgtcgt tggcgatgag ggccgcgatc atggggctga tcacgctgaa
     4801 tccggcgaac agcacgaaca cattgcgcgt caacgccgac aaggctactt ccgcgctcca
     4861 cgtaaagacc agaccggtca gcaccatcat ggcaaatttg gagcgtccga agatcggcgt
     4921 gtgccgtatc aagatgcgcg tcacgaaata gtatgtcgcc acgccgatca ggcccaccac
     4981 cagcaagtgc tgcggtcgca gcacgaacaa cgcggcatag cccgcggtca gaaagccccc
     5041 gctgcggatg ccggcccatt caaacatcag ggcgctgacg atgacgctga aaatcacggc
     5101 gggaatcagc agttgcgtct gataggaata cacgatcgtt ttgtcttcga acgcaatgct
     5161 gttccaatag atcggcagat tgtttttgat cgccaggaaa ctttgcagca gcaggtaggt
     5221 cacgaatgat gtgcccagca cggtcaacac ggtcagccgc atactctgtc gttccatgtc
     5281 gtgcgcgatc aagccgggca gcacaaaccc cacgccatac agacccacca gccacggcgc
     5341 atcggcgctg ccgaaaaagg cgatgaagcc gatcgtgaac tggaaggcca tgccgaccag
     5401 caccatcatg accaggcggc gtcggccata taagaacatc ttgcgcgcga cgaagtgatg
     5461 caccacgtaa tacgtgacga tcgctaaaag gacggtcgtc acgatgtaca gcgggcggcc
     5521 aatgaacata ctgagataac ccgcaatcac cgccgcgccg cccgtcacgc gccagcgttc
     5581 ataagccagg atgctcagca gcacgcccag cgccagcgcc agtctcacat cttcagtggg
     5641 aagcccgtaa ttgtgcatgg actcaattcc tcaaacgccg cgggtcattc gtcgtggttc
     5701 aattgtcgtt cttcgttatc ccacatgcgc catctcgaag cgtcgccgcc gcaggtagcc
     5761 gatgtcgcct tcaagttctg tgggcgtcac ggcgtatgtg tccttcaagt gctcgaagta
     5821 ttccattagc atctcggctt gcggcgtgtg gatgttgacg aagccgacaa gcatggcctg
     5881 ccatccctcc ggaatcatgc ccgcgatcgc gtcgataatc tgctcgatgg tgggactcac
     5941 gctgaaaccc agattcacga tccgttcgcg cgggaagttg ttgctcagca gcttgcgcgt
     6001 gaccaaatcc tcgtaggcac cgaaggtgat cagccagtcg aacttcaaat cttttgcggc
     6061 cacgtcgccg aattgttttg cgcgccgctc gcgatcgtag cggttgttga gaatgccaat
     6121 gcggaccgtc ttgtcgtcgc aatacgcctc cagcatatcc aggcttatga tcatcgactc
     6181 gcgatcgttg acggcgaaca aattcgccca gatcacaggc ttgccgcgca aggtcacgcg
     6241 ctgcaagcgc accacgccaa tgtcgccttt tgctttgatc atgccgcgca tcgccacgtc
     6301 ccgatcgatg ccgagcaaat cggccacggc cagcccgatg gcgacgttgt ccttgaacga
     6361 cagataatcg aactgcgcga tatcgtcgtc gcgcacccgt atgggatcgg ccaccactaa
     6421 ctgcgagttg cgcttctcgg cctcacgtct cagcacgacc tgcaagtccg ggtcatactc
     6481 cgccgtgaca agataaccgc cggtggggca catgtgcgcc aggctgaccg cgatctccgg
     6541 cagcgtttcg cccatcacgt cctgatgatc ttcgcgcaca ttcgtgatca cgccgatgtg
     6601 tgaccggatg accatattct cggtccactc ttgaaatttg ggctgcaggg ccatacactc
     6661 gatcaccaac gcttcggcct gctgcgattc ccatctggcg atgatgtcct gctgctctct
     6721 tacattcgcc gcgccgcgcc gtggaatggg cacctcttcg ccgtcgaagt tgatggtcac
     6781 tgccgccgag ccggtggttt tcgccagcgt gcgataaccc gcctcgcgca gaacgccggc
     6841 aatcaagcgc gtgacggtgc ttttgccgcg aatgccgttg acgtgcacgc gcactttgta
     6901 gtggggcaag cgcttttcat gcgcggcctg ccgccgccgc caataagcga tgaaaacgag
     6961 gtagatgatg agggtcaggc cgagttcaaa caccgctctt actcctgcgc cgcgcgcgct
     7021 ttcgccaaca gcgtctcacc cgtctcgctc gtgccattca gcgacaccag gctgacatgc
     7081 agttgtcccc attcgcggat gggcttgccc agcgcttgat cgccagtggg cggaaaataa
     7141 acctctcgct caaaacgctg ctgcagcgcc gcgatcacgg actgcgcctg cgtcgtatct
     7201 tgttccggca aaatcatcaa cacttgatca tccccggcgt gcgtgatcaa cgtgggcggc
     7261 tgtgtcgcgg cgcgggccac acgcatcacg gcttgcagcg cggcgatgcg gtgagtatgc
     7321 tggccggtcg ggacatccgg gtttgcctgc aattgcagca gcgcgatcga cagcgggcgc
     7381 tggaaacgct gcgcccgcat gacttcttcg tcgagccgcg tgagcgccca ggcgggcttg
     7441 agtgtgccga aggattcatc gtacacgctg agttcttcgg cgcgctccaa ccaccgatca
     7501 gctttgcgct gtacgcgact gatcgatcgc cccagtctgc ccgcgagcgg tccgatcaac
     7561 aagaacgcgg cgagttcggc ggcgatgata gcgacggcgt tttcgcgcgc atagatgccg
     7621 gcgtattgat tcaataatgt gatcgtcacc acgcccgcgg cggctgcgac tagccctccc
     7681 cacgggccgg tgagcaggcc tagcgcgatt gtgccgaaga gcagcgctaa ataaaccatc
     7741 gcggcttcca gcggattcac catgacgatt aacaccgtcg cgacaatggt gagcaaagcg
     7801 agcatcactg cccaaccgcg cgtgcgtgca gccttagtca tcttcatgtt cggactcctg
     7861 ctgtcgaggg gccgtccgcc ttcgagacaa ccacactccg gccacggcca cgaccacgac
     7921 gaggccgagc atgatcggcg ccatgacgcg gcgtgccgcg ggcgacaagg cgccttccca
     7981 tccgccgagg gtgaggcccg tcaagccttg cgaattcaca atcgccacgt tgccgtgccg
     8041 ttgtaggagc tgacccagca caggcaagct ggccaacgtg cgctgcatga tcgtttcgtc
     8101 gggtgcgctg atcgtgatca aggtatttcc cgccgaccac ggcgcattca gcaattgcac
     8161 gtatccggcg gagttcgtct gttcgtccgg gtggagttcg cgcccgctgc tgggttggat
     8221 gacaccgttg ctgcgcgtta gaggctgcgg caattgcgca tcgtattgct gcaagacttt
     8281 gttcgtttct ggcaggccga tggcgatagc gtgatcgccg ggacgcacgt cctcacgcca
     8341 ggcatcatcc atggaggcgc gcggacgcag accgtcggcc atggagaacg tgccgagctt
     8401 ggcagcgagt acgaccagcg ccgatagatc gctcgcggtc ggctgcgcgg acagcacgaa
     8461 gaggctggcg ctgagatcgt ccggcgtggt caggggcgaa ggccaatagc gcaagtccag
     8521 gggcggttcg ctgttaacgt gtgccaggtt cagcatcgac gatgcggcgg aaatgctggc
     8581 ccacgcctgg tcgagatagc ggaaattgca gtcttgattc ggcagacgca gatcgaactt
     8641 caggtgcagc cagttgcgcc ccggccgaac cttgcgcggc gagacttgaa tcgtcaggcg
     8701 gccatcgtcg gcgttgcgat cggtcaagtg caccaccgcc actcgcaaac cgttgatgga
     8761 cgctctcatg ttcgattcgt cggctaaccc gcgggcgtgc gcaaatctca attcgatggt
     8821 ggcatcgttg gtgatcttcc agtcgtacgg cacgtcgatc ggatagtaca gatcgtgcag
     8881 accgatgccg ttcacctgcc gatcggcgaa accggcagcg gcgaaggtga ctagtgcggg
     8941 gcgctggggc acaggctgcg ccgtaaattc ggttggattc acgtgtgtcc cgccgagttg
     9001 cgctcgcgcc gattgcgtgg taagcaaagc gaccgcccgc gacaattcgt ccgcatctgt
     9061 gcccgacacg atcaacatca cgcgctgagg attccgcggc gaagtctgca gcgtcacgcc
     9121 cgccgaggct tgctgcgcca gcggatttcg ccgggcggtg tcgatcgcga tgagatgatc
     9181 gtccgtgcgc gcttcgtcca actgatcgat cgtggcggcc cttacgggcg gttctttcca
     9241 ctccgcggcc tgtcccaacg cggcggcgat ctgcgcagcg gcggtgagtt cggcggaggt
     9301 cggtcgctcg ggcagcacga tcgagatgtg cggactgccc gccagcgtcg agagcgtcgt
     9361 gaacggcgcc ggataacgcg ccagatcgag cgacggcggc acgtcctgcc cctccaactc
     9421 gatcgccgtg tcggcgaaga tttgggtcca caggttgttg tcaccgagat cgccgcagcc
     9481 ggttgcttgg agacgttgat tgaaccgaaa cagcaattga ttatcgccag gccgcagcgc
     9541 cgcgatcggc agtggaatct ccagcgtcgt gctgaggttg tctgttgtca gcggcaaatt
     9601 gatcgccggt tcgccgttca gggcaatcgt cacgccggac cgatcgggca gcagttgctc
     9661 gtcgtgcgcg aggtgcagct tcagcgtggc ccgctcaatg cgttttcctt ctggcagcgg
     9721 aaaatgcagc gtagcttgat cgaagatcac gttcactacg atgtccttct cgccgagatc
     9781 ggccagcgtg aagcgcaatg gttctgcgcc ttgcgccagc acgccggatg gccacagcag
     9841 cagcaagaca aagcaaagcc ggatcggttt ccgcaccatg acgtttctca cggcaatttc
     9901 cgcagcgcgc gccaggccag atcggccaga tgggtttgcg gcgcaagttc cagcacttgc
     9961 atcagatcgg ctcttgcttc taccgtgtga tccagcttgc gatgcgttaa agcgcgatag
    10021 taatacgcgt agacccgcgc cgggtcgagc gcgatagcct gactcaagtt gtggagcgcc
    10081 tgatcgaatg cgccgccttt gtacagcatc agccccagaa gataatgcgc gcccgcgtgg
    10141 cgcggatcga tctgcaccag gcgattgaga tcggcctgcg ctttgacgaa gtccggcagt
    10201 tgatagtaca gccgcccgcg tgcgtagagc gccgccggat cgctcggctt aatggccaac
    10261 gcctgcgtga gatcgtcgag cgcgtcgtta acccgaccgg catcgcgcgc gatcaggctg
    10321 cgatagaaat aggccgaggc attgcgcgga tcgatccgca gcgcttgatc gaaatcgcgc
    10381 tgcgccagat ccggctgtcc caggtgatat tcggcgaggg cgcgttgaaa gacggtgttg
    10441 gcgggcgcgc tgccggcttt gagcgccgcg ctgaaatcgg cgctggcctg cgcgtaatcc
    10501 cgcagatgat aggccgccag gccgcgatag taggtcgtca gcgtggtggt gcggccgctc
    10561 ttgatcgccg cgtcaaaatc agcaaaggcc ggggcatatt gcttcaaatg atattgcgcc
    10621 aggccgcgct gatacaactg cgcggccgtc ggagtgaggg attgaaaagc ggtgctgaaa
    10681 tgggaggccg ccatcgcgta gtcttgcaga tgataagccg tgagtccggc gtaatagcgt
    10741 gccttggccg tataaggcga cgtcccttgc gccacggcgg caaaatcggc ggctgctgat
    10801 tcatagtcgc ctgcgcgata gagcgccagg ccgcgttgat agcgctgtgt gtcggtcggg
    10861 gtggtatccg ccagcgccgc cgtgatgtcc tgcgcggcct gcgcgtaatc ctgcagatga
    10921 tacgcgacga ggccggcgta atagcgagcc gagagcgcgt gcgttgagct gcctttggcc
    10981 acgctttcaa aatccggcgc ggcccgggcg tattgcccca aacgataaag cgccaggccc
    11041 cgccagtagc gcgcgctgtc gtcgtcgggg ttgagttgca gcgcttgctc gaaatctgcg
    11101 agggcgcggg tgaaatcgcg gcgcgcgtag taaatccgtc cgcgttcgcg atagggcgca
    11161 gggcgctgcg cgtcaagctg cagggcgcga tcgagatccg gcaaagctcg atcgggctgc
    11221 tgctgatgga tataagccag cgcgcggcca taataggcgt tggcatcggt cgtgttcagc
    11281 cggatcgctt gatcgaagtc ctgcgcggct ttctcgtact gctgcgagtg atagaaggta
    11341 tagccgcgcg cggtgtacgt ggcggcatcg tcgggctgca gcgccagcgc ccgatcgaaa
    11401 tcggcttgcg cctgcgggta atgtccctgt gcgtcgtagg ttttggcgcg ctgcacgtag
    11461 accgccgcat acgtagggct gatcacgatg gcctgattga aatcgcttag ggcttgatcg
    11521 tactgccggg ctttgaggta agccaaaccc cgatcgtaat aaccgcgcac cgagcgggca
    11581 tcgctgcgga tgccgcgcgt gaaatcctga atggccgctt cgtattgtcg cagtcgatag
    11641 taagccaggc cgcgatcgaa gtaggaaccg gcgcgcagcg gcgagcgttg aagcgcttgt
    11701 gtcagatcgt cgatggcccc ggcgttgtcg cccagcttca agcgggcgcg tccacgtccc
    11761 gcccaggttg aagcatccgt tgtgccgaat ttcagggcgg catcgaaatc ggcacgggcg
    11821 gcttcagcgc tttgcaggtt gaggttggcc cagccgcgtc cggtccatgc ctcgtgcctg
    11881 tccggttcga tctgcagcgc ggcgttaaac gattcaagcg ctgcgcgcca atcgcgatca
    11941 cgcagcgctt gctggccagc gtcgatatag gaccgggcat tcggtgcagc gagagcgccc
    12001 gtacaggccg cggccaacca gacgacaagc aacagccagc agcggcgcgc ttcaggcagg
    12061 acgcgtctgc atgaggagtt catcccccat cctgttaatc acattcaacg tgttcgggcg
    12121 atggataggc caatgccagt ctgacgcaca ccagtgctgc tgcaaagtgt aacccagtaa
    12181 agttagattg cggcgagaaa cctgttagag agtgtcttca aatcgccgat cagcacgttc
    12241 aggacctgcg gcagcgcgtc cagagatagg cggcgcagaa gccgggcaac tcgggtgatc
    12301 tagggatcgt cggaaatctt gaagaataat ccatcgtcaa gctctctggc acaacaaatc
    12361 tccgaaatcg gccctccgct ccgatcggca caggtttgct ggcggtcatt tgtgttgcca
    12421 gttcgtaata cctctatatc ctataccgca aaaaaccgtt ttacgattcg cttcatttgt
    12481 tagcgtttgg tatgaaatgg tatgccaaac ctcggcaggg tagcggacag agcgtccttc
    12541 ctaccgaaaa taacctctgt ccacaccgga atcacacaaa tgcaacggct gcggcaactc
    12601 ccggcgctgg cgccctcgcc cgatgctcca ctttttcgca tctgcccccg ctggatgatc
    12661 gatccggcgg cctgggagcg gcggattgat cacccgaaga ggccgcgaac gcggtccaaa
    12721 ggcagcacaa ggtgcgaaat ccgggcttat gccgcgaagg agcggttgtg agacctggca
    12781 atgtgaatga cacgcgttcg ttgaggagta cagtcgcgtt cggaggctga aagtgttggc
    12841 ctggaatcct acctgccagc gtgaaaaact gaacgaattt gctgaaagtg tgacttcagt
    12901 cctaacaatc atggccgcct tctgctaact tgtatggcga tagcgatctc gctggtttct
    12961 ccgggtggag ggcatcatgt ccaatctctc gcgacggcag ttcctgatca cgggtggcgt
    13021 cacgacggca ggcacgctgc tcggcgcgaa gctcggcttg cgtttcctgc agcccatcgt
    13081 cgacgtgccg aacccgctgg cggcttatcc cagccgcgac tgggagcagg tctatcgcga
    13141 ccagtatcgc tatgattcca ctttcacttt tgtctgctcg ccgaacgaca cacacgcctg
    13201 ccggctgcgg gccttcgtcc gcaacggcgt catgatgcgc gccgagcaga actacgacgt
    13261 agcccgctac gcggacctgt acggcaacac ggccacgccc cattggcacc cgcgcggttg
    13321 caagaaggga cagacctttc atcgccgcgt gtacgggccg taccgcctga aggggccgct
    13381 gatgcgcaga ggctggcagg aatgggccga cgccggattc ccgtatctcg acgcggcgaa
    13441 caagagcaag ttcaagttcg acgcgcgtgg ccaggacgag ttcctgcccg tcgattggga
    13501 caccgcctac gactacatcg cgcgcggcat ggtcgccatc gcgaagcagt acagcggcgc
    13561 agaaggcgcg cgcctgctcg caagggaagg ctacccgccg gagatgatcg aggcgatggg
    13621 cggcgcgggc acgcgcacat tcaaatgcag gggtgggatg ggtttattag gcgtaattgg
    13681 taagtatggc atgtatcgct tcagcaacac gctggcgctg ctcgacgtgc acgtgcgcgg
    13741 tgtgaatcca gaggaggcgc tcggcggccg caactggagc aactacacgt ggcacggcga
    13801 ccaggccccc gggcatccct tcgtccacgg cctgcaggcg tcggatgagg acttcaacga
    13861 cctgcgcaac agcctgctgc acattcagtg cggcaagaac ctggtcgaga acaagatgcc
    13921 cgattcgcac tggttcgtcg aggcgatgga gcgcggcgcg aagatcgtca cgatcacgcc
    13981 ggagtacagc ccaccggcga cgaagtctga ttactggatt ccgatccgcc cgcagaccga
    14041 cactgcgctg ttcctcggcg tcacgcgcct gatgatcgac aacggctggt acgacgtcga
    14101 cttcgtcaag cgcttcaccg actttcccat gctcgtgcgg gccgacacgc tgaagcgcct
    14161 gcgcgccgcc gatctcttcc ccgattacca attacaactc accgccgaca gcgcgtcctt
    14221 tgcgctgcag ggactgacgc cggagcagta tgagatactc ggcgactacg tcgtgatcga
    14281 cgcggcgacc gggcagccca cggcgatcac gcgcgacgac gtgggcgaca agatggccga
    14341 gaagggcatt gacccgcggc tcgactatcg cggcgcgctg acgctcgccg acggctcgca
    14401 agtcgaggtg ttaacgctgt ggtcgatgta cgtcgatcac ttgaaggact acggcctgga
    14461 cgccgtggcc gaaatgacgc acgcgcccaa ggacttgatc gagcggctgg cgcaggacat
    14521 cgccacgacc aagccgacgg cgattcacat cggcgaggga atcaatcact ggttccacgc
    14581 cacgctcgcc aatcgcgcgc agtacctgcc actgatgctc accggcaaca tcggtctccc
    14641 cggcgcaggc tgctacacgt gggccggcaa ctacaaggcc gcgctgtttc aaggctcgcc
    14701 gtggagcggt cccggcttca agggctgggt ggccgaagac ccgttccagc cgagtctcga
    14761 tccgaatgcc tctggcaagg acgtgcacgc gcacgccttc acccaagacg aggagccggc
    14821 ctactggaac cactctgagc gaccgctcat cgtcgacacg cccaagtacg ggcgcaaggt
    14881 gttcaccggc acaacgcaca tgccgacgcc gacgaaggcc atgttcttca cgaacgtgaa
    14941 cctgatcaac aacgccaagc acgcctacga gatgatcaag aacgtcgatc cgaacgtgcc
    15001 gctgatcatc tcggtcgaca tcgagatgac ggcgacgatc gagtactgcg acttcgcgct
    15061 ggcggcgaac tcgtgggtcg agttccagaa ctacgaggtc acggcgtcct gctccaaccc
    15121 gttcctgcag atctggaaag gctcgctgcc gccgctgtac gacacgcgcg acgatgcagt
    15181 catcctggct cagctcgccg ctaagctcgg cgaggtcacc ggcgacccgc gcttcgccga
    15241 ctactggaag ttcgcgctcg agaaccgggc agaggtgtac atccagcgtc tgctcgactc
    15301 gtcgacgaca acggccggtt acaggttcga ggacatcatg gccggccagt acggcgagcc
    15361 gggcgcggcg cttatgctct tccgcaccta tccgcgcatc gcgttctggg agcaggtcca
    15421 cgacaacgtc ccgttctaca ccgacaccgg ccgcctgaac gcctactgtg acatccctga
    15481 agcgatcgag tatggcgaga acctgatcgt ccaccgtgaa ggcccggagg cgacgccgta
    15541 cctgcccaac gtcatcgtcg cctcaaccga cttcatccgg cccgacgatt acggcatccc
    15601 gctcgacgcg atgggctggg aggagcgcac ggtgcgcaac gtcaagatgg cgtgggcgga
    15661 tgcgaagcag acgaagaacc cgctgtggga aggcggttat cgtttctact gcgtcacgcc
    15721 caagacgcgc caccgcgtcc actcgtcgtg gtcgaccgtc gactggagca acatctggga
    15781 cagcaacttc ggcgatccgt accgcgtcga caagcggctg ccacacgtcg gcgatcacca
    15841 gttgcacatc catccgcagg cggcgcgcga cctcggcctg gaagacggcg actacgtgta
    15901 cgtcgacgcc aatccggccg accggcccta catcggctgg aagcccgacg atccgttcta
    15961 cagagtcgcg cgcctgatgc tgcgcgtgaa gtacaacccg gcctatccct accatattgt
    16021 gatgatgaag cacgcgccgt tcatcgccac cgagcgctcg gtgaaggcgc acgagacgcg
    16081 gcctgacggg ctggcccgct cggaaggcac cggctatcag gcggacctgc gctacggctc
    16141 gcagcaatcg gtcacgcgcg actggtcgat gccgatgcac cagaccgaca cgctgttcca
    16201 caaggcgaaa gtcgctaccg cgttcatctt cggcggtgag gccgacaatc acgccatcaa
    16261 cactgtgccg aaagagacgc tggtcaagat cagcaaggcc gaggatggcg gattgggcgg
    16321 ccagggcgtc tgggagccgg cgaccacggg ctattcgccc ggcaacgaga gcgacttcat
    16381 gcgccgctac ctgtcgggcg atctggttga gacgtagatg gaggcaggcc gcgcatgacc
    16441 gacacgtcac tccctgccca atgtatcaac tgcgaacgca gcgtcggcga ggtgccattg
    16501 atcccgatcg tgcatcgcga cggcccggcg cacatttgcc cacagtgcct gccgatcctg
    16561 atccaccagc cggacgcgct gaccggcaaa ctggccggcg tcgaatcgct ccagccgcac
    16621 gagcattgag gcgaagcatg cccaaggacg aattcgagca cgacgatccg atggaactcg
    16681 tcggcatgat actacccggc gacgaggcga cgaccgaggc gatggccgag accttcgtcg
    16741 aagagtatgt gcgcatgggc tgggacgatg cacgcttgat gaagctgttc actcatccgt
    16801 tttacgtggc aacccacgcc gtctaccggc tcaagggcga agcctacgtg cgggagttaa
    16861 tacggaaaac atgcgcgaag tggcaacgaa taggcagcgg ataaacggat tcgctgactc
    16921 gctgattcgc tgatctgcca accgaccatc caaccagcaa acgtgtcgca agccggcacg
    16981 ctcgaagagg agaactatgg ctgaggtcta caactggcaa ctcgggcgcg cgatggccta
    17041 tccgttcgcg gaggcacatc cgcagaagca gttcgtcgcc gtgttcaaca tcaaccgctg
    17101 catcggctgc cagacgtgca cgggcgcatg caaggccacc tggacgttct ccaagggcca
    17161 ggagtacatg tggtggaaca acgtcgagac taaaccgtac gggggttacc cgcatcactg
    17221 ggacgtgaag ctcctcaacc tgctcgatca agcgcatcag acagcaggca agaagatgtc
    17281 gtggagtgcg ccgaggcgcg acgaggcggc cgcgccctat ggcgtatacg acggcatgac
    17341 gatcttcgag gctgccgatc cgaacaacaa cgtgctgggt tatctgccca ccgacggcga
    17401 gtggcgcgcg ccgaacattt tcgaggacgt gtcgccggca taccgaggcg acgatctggg
    17461 catcagcatc gaaggcgcat cgctgcccga gcacaagacc tggttcttct atctccagcg
    17521 catctgcaac cactgcacgt atcccggctg cctggcggcg tgcccgcgca aggcgatcta
    17581 caagcgacct gaagacggca tcgtcctgat cgaccaggag cgctgccgcg gctatcgcaa
    17641 gtgcgtcgaa gggtgcccgt acaagaaggc gatgttccgt ggcacgacgc gcacgagcga
    17701 gaagtgcgtc gcgtgctatc cgcgcgtcga gggcagcgat ccgctgacgg aaggcgtgcc
    17761 gatggagacg cgctgcgtcg cagtgtgccc gggcaagatc cgcctgcaag ggctggtgga
    17821 catcgccgaa gacggggcgt gggtcgagaa ccggcaaaac ccgctgtact tcctggtcaa
    17881 ggtcgagcag gtcgcgctgc cactctatcc gcagttcggc accgagccga acgtctacta
    17941 catcccgccg cgttgggtga atcgcagctt cctgcgccag atgttcggac ccggcgtcga
    18001 cgatgccatc gagcgttaca cggcgccttc gcgcgaattg ctggcggtgc tgcaactgtt
    18061 tcgcacgacg cagaaaatca tcttccgtta cgagatcgaa gaagggccgc tggtgcacga
    18121 gactgaggtc aacggcaagc cgtggtcgat gtacaacgac accgtgatcg ggttcgacgc
    18181 agacgacaag gaggctgtgc ggctgtccgt cgtcgagccg attcacgagc ggccgcctga
    18241 acggcagaat tcgatttaga agtcggaggt tggaagttgg agattggaga ttagagatgg
    18301 gaggttagag gttggaggtt ggagatggga agcgagcaca gagatccaca ggtgtttcgt
    18361 atactgtgcg gcgacgccgt agaaaccagg cacacgcctt acggcagcgt cggaacggtt
    18421 ttcagcggtg agggcatcga ggctgtctgg gtgtctaagc aagacgaagc gatcgagcct
    18481 ggctggtttt ctcaatccac ggtcgatctc atcctggtgg tgcaaggcca acttcacgtg
    18541 gaatttgagc ggcccgatct ggctcctcgg gtattggagc cgggcaatct gctcgtcctt
    18601 cccgcgaaca ctcgttgtcg cgcctatcgc tggccgcgtg accgaaaaga ggcaacggtc
    18661 tttctcgccg tctatcctgt aaagcaggag taggggagtg gtggaatgaa atcgagcaca
    18721 gtcgaagcca cgacagaaga aacctttggc cgcgcgcagg cctaccgcct gttcgccgac
    18781 gcgctggtgt atccggacga ggctttcgcc gaacggttag ccgacggtta tgcggccgat
    18841 ctgctggaat gcctcacgat gcttgacgcg tcgtgcgata tggcctggag cgagccattc
    18901 gagcgactgg tccgcgccct cgccggcgcg acgctcgccg atgtgcagcg cgcgcatcgt
    18961 ctggccttcg gcgtggcggc cggcacgccg ccatacgaga ccgagtacgg ccagccgcac
    19021 gcgttccggc agtcgcagga gcttgcggac atcgtgggct tttaccgcgc gttcgggttc
    19081 gacgtgggcg gcggcgtgcg cgagcgcccg gattatctcg gcgtcgaatt ggagttcatg
    19141 tacctgctgg catgcaagca ggcttacgcg gtcgccggag gattggccga cgcggccgag
    19201 gtgtgtcacg acgcccagcg caaatttctg cgtgatcacc tggcgcgctg gatcggtatg
    19261 tacgcgcgcc ggctgaacga tcaggcgcag ggacgcaatg catacgcagt gctggccgat
    19321 ctggctgctg cgttcgtcgg cgaagatgcg cgccggttgg gaatcgagcg cgactcgatg
    19381 gcgccgggcg acgcgcggcc gaccccgcca gcgctttcgc tttcgtgcat gggctgcaca
    19441 gaagaagatg aattttcatt ttgatccccg gctgatggag gaggcggtcg gcgcggcgct
    19501 ggcgcggcgg ccgatgctgg ctgcccgcta ccgggccggc gtcgaggcgc tgtacgtcga
    19561 cgtcccgctc ggcgagcgcg agccggcctt cgcgcgcctg catgcgaagt ggttcgagtc
    19621 gctcgaactg gatcgcccct tgcgcgccat cttcgaagag tttccggtcg tcgtggcggc
    19681 tgcccgggac gtcatcgtgg cgcgggccga tcggcggcgc gacgagggcg cggtgctcgc
    19741 gccgcgcacg acgcgcctgg gcctgcggct gacgccggct cgcttcacga atgacgatgc
    19801 gctgtcgtgc ctgatgcgcc acgagctgac gcacgtcggc gacatgctcg atcctgagtt
    19861 cggctacccc gatcgcccgc ccagcggcgc gacggcgggc gagaacctga ttcgcgatcg
    19921 gtatcgattg ctgtgggatc tgagcgtcga tggacggttg gcgcgcgcag ggaagaagtc
    19981 catcgcagac gcggcgatgc gccggaggga gctagacgcg ctgtattgca gcctgcctga
    20041 ggcgactcgc tcggcgatct tcgatcggct gtggggcgcg accgacctga cgcacgatcg
    20101 attgtgggct gtggcctctg acgtgcgcgc gcttgtcgca tttgccggcg tggccctggc
    20161 agcaggcgcc tcgaacgggc cgctgcccgg cgcgcgctgc ccgctgtgca ggttccctac
    20221 ttacgtctgg tccccgccgc tcgaaccgaa ggtcgtctcg atcgtgcggc tcgatttccc
    20281 gcgctggcag cccgacgagg gagtatgcga gcggtgtgct gagttatatc gaatgcgggt
    20341 gggggattgg gaattggaga ttggagttta gagattggag attagagatt ggagattgac
    20401 gatctccgtt ctccgatcgc cgtgtaacta gaaatgtcag attcggcgcg gctaatgccg
    20461 ccggggacgg cggcttgagc ctaaagtact ccgatcgctg tgcaaggagc aaggctatgc
    20521 tcaatcgaac tcttcacttg cgaaactgga cacgcgccct gctgccgata gcgctgctgc
    20581 tggcgctggc ggcgacggcg ctgcgcgtgc cgacggcgtc gtcgcaaggc gtgacgatcg
    20641 tcgccaggca agtcgaagcc gatttgccgg tcgacgatcc cgattcgccg gtctgggagc
    20701 aggccgttcc cgtcgaagtg ccgctggcgg cgcagacggc ggccgtgccg cgctggccga
    20761 atcccggcgt ccaggccgtg accgttcgcg cgctgcacga cgggtcgcgc gtcgcgttcc
    20821 gcgtcgagtg ggccgacgcg acgaaagacg agcacatgct gcgcgtggat caatttcgag
    20881 acgcggtcgc catccagttt gcgctcgcca aaggccagcc gttcttctgc atggggcagg
    20941 tgggcggtct ggtgaacatc tggcagtgga aggccgattg ggaggccgat ctcgccgggt
    21001 accaggacat ggaatcagtg tatccgaaca tggccgccga cgattatccg ctcacggagg
    21061 cggagcctgg agaaatcgcc acggtctatg aatacgatcc gacctacctt gctgcgctcg
    21121 ccgccggtaa tctgttcgcg tcgccgcagc ggatcagctc ggtcgaggac ctgacggcag
    21181 gtggcttcgg gacgctcacg gcgcagggca gggcggggca gaacgtgcat gggaaaggcg
    21241 tatggcgcga tgggaagtgg cgtgtcgtgt tcgatcgcgc gctggagtct gacgaagccg
    21301 acgacatgac cttcgagctt ggccaggtcg cttcgattgc gttcgcggct tgggacggtt
    21361 cgaacgggga gcgcaacggg cagaaggcaa cgtcgaactg gtattcgttc cagcttgaag
    21421 gcgaagcgcc cgcgccgccg gctcagtggg cgccgcccgg cgcgccggag ttggtgcaga
    21481 tgcttgcgtt tgtcgcgggg atcgggctgg cgatttggat catggcggcg gtcgtcgacc
    21541 ggatggggcg gagccgaggg gtgtaatcgt gcgctggcag aactggctgc tcgccgcagc
    21601 gctggccgac tggctgatcg ggcggacact gtcgcgcgcg gcgatcttca tgcccaaatc
    21661 cggcatcatg gtcaccgtga cggaaggcat gatgacctcc gggcagatcg cgttcaatct
    21721 ggccgcgatc ttgacgctgg cggcgatcgt ctggctggcc gtgcgcggcc tgcggcatgt
    21781 ggagcacaac ttgcccctct cggcggcgct gtcaggatta gttgggctga gcatcgccgg
    21841 ggtgttcgtg ccgcccggcc cggtcacgct gatcgtgttt cagatgctca cgctcgccgc
    21901 gctcaccatc ctggttgtgg aaagcaggcg cgcacagccg gactgcagtg cgcggtgggc
    21961 cgtagtcccg cctgtgctcg ccatcgcgtc gatcacatcg ttcaagatgg ttcagacggt
    22021 ggcgcaattg acggacgcgc cggcgccggc gtcgctcgca ctgctcgctt ttcaagtcgg
    22081 tgagcttttc gtcgtaatca cgcccccggc cgtgtggtgg gcatacggca agaccggccg
    22141 ggtaagagat tgggtaattg ctgggattcc cgccgcgctg ttcacgatcg cctacctggc
    22201 gaacccatcg atgacggcga caatggcaac gtggtcggtc ggcctcagcc tgtatctgcc
    22261 gtggccggtg tacgcgatca gcttgtggct tgcggcagtg acggtcctgc gcgcgctgcg
    22321 cgccgggcag ggggcgggct atgggctggc atatctgacg gctgccggat actccatgca
    22381 gttgagcagc caggtgatgc tcggattggt aggcatatgg atgctcggcc tcacgagcct
    22441 gtcagttggc cgttttgttt ccccgcccct cagggcgaat aaaccccttc aatccatcat
    22501 ccggtagatg acgtctcgcc cgagacaatc cttttcgggc cgctgcccga tcaggtcgcc
    22561 ctgcacggcg tgctgatgcg gatccgcgac ctgggcctca cgctgatctc ggtcaaccgg
    22621 atcgaccccc agcgagtggc aggctcgaca tctaaaccgc actaatcgga gtgcagaagt
    22681 caggtttgga atgcagcgca agaccgcggt gcagtcggtc ttgtctctgg tctaatgcct
    22741 gtcgcagcca gctttcggtc agcgcgtcaa gcgcaatgtg gagtattcca ctccacaatg
    22801 gcttcgtgca attcggcctt tgtgctgagc atagattaca ccttcgtcct gatgagccgg
    22861 cgtgcgtttg tctgtatagt aaggcgcggt ggagccacag aaagataatc aactcacacg
    22921 tatgacgtgt gatgagcaga gagagccatg tattcccatc caacagctat agtgatcggc
    22981 gcaggactcg gcggcattgt ggcggcggct cgcctggcgc gggccggcta ccaggtgacc
    23041 gtgctcgaaa aaaacgcgac acccggcgga cgctgcggcc agttgattcg cgctgggcac
    23101 cgcttcgaca tcggcgcgac gctgttcctg atgcccgagg tgttcgggca gacgtacgcc
    23161 gagctcggcg agcgcatgga agaccacctc gacttgcgcc gcatcgaccc gacctaccgc
    23221 atccacttcg ccgacgacac gacgctggcg ctgacgaata atctcaacga tatgcaggcg
    23281 cagctcgaag cgatcgagcc tggcagtttc ggcgggttgc tgcgctatct ggtcgaaggt
    23341 gagcggcact accacgtctc gctcgagcgt ttcgtcgggc gcaacttcta caacctgctc
    23401 gactacttca gccttcagaa cctgccgctg ctgttccagc tcaaggcgtt gaccaggcac
    23461 tacgacaacg tcggccacta cttccaacat cctcagctca aggctgcctt caccttccag
    23521 aacatgtacc tgggcctcag ccccttcgac gcgcccgcga cctactcatt gctccaatac
    23581 acggagctgg ctgacggcgt atggttcccg atgggcggcc tgtatcggat cattgagagc
    23641 cttgcctcga ttgcccaggc acacggggta gagttctggt atcgctcgcc ggtcaagcag
    23701 atcgaggtgg tgggcaaccg cgctacgggc gtcgtgttgc agaatggcgc gcgactggac
    23761 gcagatgtaa tcgtcgccaa cgccgatttg ccgtacgtgt accgcgacct gctgcccgac
    23821 caggacgaag cggatcgcct ggagcgcctg aagtatacct gctcggcgat catgttctac
    23881 tggggaaccg accaggttta cccgcagctt gggcatcaca acattttcct ggccggcgat
    23941 tatcgcggca gtttcgatcg tatctttcag gatcacacgc tgcccgacga acccagcttc
    24001 tacgtccacg cgccggcgcg caccgacccg gccgctgcgc ccgccggcca cgacacgctg
    24061 ttcgtgctcg tcccggtcgg ccacctggac gagtcccggc cgcaggactg ggatgcgctg
    24121 aaggcgaagg cgcgatcgac ggtgctggat cggctcagcc gcctgggcct gagcgatctg
    24181 gagcagcacc tgaagttcga ggtgagcacc acgccggtga attggatgaa tcgcttcaac
    24241 ctggcgaagg gcgcggcatt cggcctcagc cacaacttct ggcaggtggg ctacctgcgc
    24301 cctcagaacc ggcatcgccg ctaccgcaac ctatacttcg ccggctccag cacgcatccc
    24361 ggcactgggc tgccgatggt gttactgtcg gctcgcctga ccaccgagcg catcctgagt
    24421 gagagcggcc agccgcgccc ggtgatgatg ataaatccgc tgcccgcgta gtataagacc
    24481 tgccaggttt ccgaaacctg gcaggtctat tggaataaac cgcgagaacc acagccatga
    24541 tcaccacgtt accctcaccc acctgggaac gcgccctgct ctcgctggcc tacgagcgcc
    24601 gttcgaccga ggccgctgtt cccaaacctg tcgccgagcg aaaactgctc gagcgcgctt
    24661 atgctcgctg cgagtcgatc acggcggcgc acagccgatc gttctacctg gcgagcgggc
    24721 tgctgccggc cgataagcgc cgagctgttc gcgcactgta tgctttctgc cgcgtcagcg
    24781 acgacatcgt cgattgtccc ggcggcgatg cggcggcgac gctcgccgcg tggcggcagc
    24841 atgcgctagc ggccgagccg ccggcgagcg acctggtggc actggcctgg gccgacactc
    24901 gactacgcta ccgcatcccg atccgctacg tcgagcagtt ccttgacggc gtggcgcgcg
    24961 atgtgcgcca gaagcgctac cggactttcg aagagctggc ggagtatgcc tacggcgtcg
    25021 cttcgaccgt cggcctgatg agcatgcaca tcatcggctt cgccggccgc gaggcgctcc
    25081 cctacgcgat caagctgggc gtcgcgctgc aactgacgaa catcttgcgc gatgtcggcg
    25141 aggactggcg cgcgggccgc ctctacctgc cggccgagga gctggcgcgc ttcgggctgg
    25201 ccgaatccga cgtggcggcc ggccgcgtcg acgatcgctg gcgcgagttc atgcgctatc
    25261 aaatcgagcg caaccggcgg ctgtacgccg aggcctggcc gggtatcaag ctgctccatc
    25321 cggatggtcg cttcgcgatc gcgacggccg gcgagttgta ccgcgcgatc ctggccgaca
    25381 tcgaagcaca cgactacgac gtgttcacgc gccgcgcgca cgtgagcggc ccgcgcaagc
    25441 tgctcaacct gcccggcatc tggtggcgca accgttgaga ggcgtgagga ttcgatccgt
    25501 cgcgcgcatc gcacaggggc gaggatcatg aaacactacg acttcgttat cgtcggcggc
    25561 ggtctggccg ggttgagcct ggcctgccac ctgatcgaca gccccctgcg tgaacgctcg
    25621 atcctgatcg tcgacaaaga cgcgaaggat aagaacgacc gcacctggag cttctggacc
    25681 aggtggccga cgctgttcga tagcatcgtg catcgctcat ggaaccggtt gcacttggcc
    25741 ggcgagaact ttgatcagat catcgacctg ggcgactacc gctaccagat gattcgcggc
    25801 atcgacttct accgcttcgc tcgacagaca ctctcggcgc acccgaatgt cgaattcctg
    25861 caggcgaggg tcgagcagat cgaggacgat gccggcgaag cgcgcatcgt cgcgaacggg
    25921 caacccttcg ccgggaagtg ggtcttcgac agccgcttca agccgccgga gctgaaaccc
    25981 gatccgaccc gctaccacac cctcaacctg cacttcaagg gctgggagat cgaagcaccg
    26041 gagccggctt tcaacccgca ggcagcgacg ttcctcgact ttcgcacgcc gcagaaaggc
    26101 gacacgcgct tcttctacgt gttgccgcta tccaagcagc gcgcgctggt cgagtacacg
    26161 ttgttctcag cggagctcct gaagcgggac gagtatgagc gagcgttgaa ggactatctc
    26221 gagaacgtat tgcaggttgg agattaccgc gtcctgagcg aagagaaggg catcatcccg
    26281 atcaccgatc agcccttccc gcgccgcgcc ggccggcacg tcatgacgat cggcgcgaag
    26341 ggcggccgca tcaagccgac gacgggcttc gccttccttc gcattcagca ggattcggcg
    26401 gccatcgtgg attcgctgct gcgtaccggc cagccgttcg acatccctgc cgattcggcg
    26461 cgctatcggc tgtacgattc gctgatgctg cagatcatgc ttcggcgcgg cgctcaggtg
    26521 cagcagctct ttacgctgct gttcaagaac aatccgatcg agcgcgtgct gcgcttcctc
    26581 gacgaggccg gatcggtctg ggagaacctg cagttgatcc caacgctgcc gcctgcgccg
    26641 ttcctggagg cgctgtttcg gctcaaggtg ctgcgtcaga tgtcccctaa ctcgatcgat
    26701 atccgggatg cgcgcaccgc gtagccgtgc ccagcatgat gcgatcgcga attgcgcaca
    26761 ctattgaatt ggttcgactg ttttccaacc tagcacatcg cgcagaatta gcgtggcgct
    26821 ggcactccaa atgccgatga ctcaggaaat tgcagttgtg caattcagaa tttggacaca
    26881 caacctccca tattcgggac aaatgtcact ttaattcgga cgcaagatgt ctacactcaa
    26941 agctattcgc atacttttcg ctccctgcct attttgtcca tcgttacacc aactggtgat
    27001 tgcacacatg ttagggcgac agttgacttg tcgcccggca tccgcagtgc aactgcggac
    27061 gaacggactt atgggtaatc accagtcaca ccaaccaact ctacgagttt cgcagttcga
    27121 aaggagttct catgtccaag atcgataccc tcgtgcaaga cttcctcgcg cagaagaaga
    27181 tcgccgtggt cggcgtttcg gataagcgcg agacgggctg caacctgggc taccgtaagt
    27241 tcaaggcggc gggctatgcc gtttccgcgg tcaatccacg cctgaccacc tttgagggag
    27301 atccgtgcta tccggacctg aagtcgattc ctgacaaacc ggaggcggtg ttcattctcg
    27361 ccaatcccaa agtcaccgag cagatcgtcc agcagtgcgt ggatctcggc atcaagcacg
    27421 tctggatgca ctgcttgatg ggcacgaagc ccggtttagg ggcgggcacg accagcgtct
    27481 cccaagacgc ggtggggatg tgccgcgaga acggaattac ggtgattccg ggcgcctgtc
    27541 cgaatcaatt cctgaagccg gatttcggtc acgccttgat gcgggtgatg ttccgcacgc
    27601 ttggttttca tagcgtcaac taaacccgcg gtcaacaaag ccgcaacgcg aacccatatc
    27661 cttaatccca ggagatcctg tgatgaagag aatccttgtg gcctatgtca caatggctgg
    27721 tttgactgtc gatgttgccc gggcagtggg tgaggagatc gcccagagcg gattgcaggt
    27781 ggatgtcctg ttgggattgt aagaccaaca tcgaaaggaa aataacgatg tcacaaatct
    27841 tgattgcctt gtccatgtgg ctgcatgctt tggggactgt catcctgatc ggacactacc
    27901 ttctgctctc cctcatttac cttccggttt tggcgaagga ccgtggaacc atcctcagcg
    27961 aaatctccaa gcacagcaga acctggctgt atctctcttt gcttatcttc atcatcaccg
    28021 gggtctatct cacgctcgct gacccgaact atttggggct tgcgaatttc ggcaaccttt
    28081 ggggaatcct gatgctggtc aagcatgtgc tgatcttggg aatgatcgca atggggttct
    28141 ggttcaatgc aatcctgcgc gtgggaccga tgatgagctc aaatagcggg gcagcacagg
    28201 ctgctatccg catgcgcttg tatgccaacc tgatggcgat ctctggagtc ctcgtattgt
    28261 ttttgacggc tctcgcgcag gtgcaataaa cgggagccaa acggttgatg cgagaactcc
    28321 gcacctgcca aagagttaca accatgaata taaaaagatt tctcttccca tcgtcgctcg
    28381 ttggcgctgt gctggcgctc atccttcggg cattcgctcg ggtattcgcc aagcccgctt
    28441 cggcaaaacc ggtatccaac agctcctcct atgatgcgat cgacgcctac gtcgaagagc
    28501 aaatgcatcg cctgaagatg cctggcgtat ctctggccat cgtcgaaggc gacaggatcg
    28561 tgcacctgcg cggctttggc cgggcccgtc ccggcggtga gcctcccttg ccccacacgc
    28621 ccttccccat tggctcactc accaaatcgt tcacggccct ggcggtaatg caactggtcg
    28681 aagccggaaa gattgatctg gatgcccccg tccagcgcta cctgccctgg ttccgcgtcg
    28741 ccgaccccca ggcatctgcc caaatgaccg tgcgccacct gttgaaccag accagcggcc
    28801 tgcccacatc gtccggggag ccttgcccgg ccgatttcga cactagcccg ggcgccaccg
    28861 agcgccaggc gcgcgtgttg tcaacgctcg tgctcacccg cccggtcggc tcggcgttcg
    28921 aatacagcaa ctcgaattac aacttgctcg ggctgatcat cgaggctgtc agcggcgagt
    28981 tgtacgcgga ctatgtccaa gaccacatct tcactccctt gggaatgagc cacagctata
    29041 ccacaaaagc cgaggcgaaa cagaacggcc tggcggtggg ccaccaatac tggttcggca
    29101 ttcccttcgc cgcgcccaat atgcccctcc cgcaccgtgg gcttgcaggc ggtgggctca
    29161 tctcctgcgc tgaagatatg gcccgctacc tgatcgctca tctgaacggg ggccgctacg
    29221 gggacgtgca aatcctctcg gctgccggta tcgacgagct gcaccgcggg gtggcggact
    29281 tccgtgctat gggactttcg ctggggcagt acgggatggg atggttcgtc gataagatcg
    29341 gccagacgaa gctcgtcttt cacggcggca ccctccccga ctttgcttcc catatggcgc
    29401 ttctgccaga gcacaagaaa ggcgtggtcc tgctcttcaa tgcctgtcag cattggatga
    29461 accccgttct ggcggacttg gggaacggcg tggccgctct gctcgccggc gaacagcccg
    29521 ctcacctccc gtttttttac gtggtcccct gggcactgcg tggccagctg ctcatcccag
    29581 ccctccaaat tgccggcgtc gcagccacgc tgcgcctgtt acgccgctgg cgcctgaatc
    29641 ctgagcgccg cccgagcggt ggacgcaagt gggggcttca tatcctgctc ccgctgatcc
    29701 cgaacctgtt ggttgccctc accctaagac cgctgctagg caagaggcgc ggctatctga
    29761 tgctctacat gcccgactat tcctggatcg cgatgatctg cggcagcttt tcgctggtat
    29821 ggagcttctt gcgcaccggg ctggtcctgc aggccctgag aaggtcctcg tagcctcttc
    29881 ccttcgttgc gcggctcggc agcgagcaga gaggctgagc acttgcatct tccattttcc
    29941 atttccctgg ttcccatgga gggcaccctg ccatgaacaa caacattggc ccgtaccatg
    30001 tcgtcgatct cacaccggcg cgccgcgtgt ggctgaacac gctcgagctg tcttggtcga
    30061 cgcattgcat gtacggcctc ctagaagtgg atgtgacagt cgcccggcga ttcatcgcgg
    30121 agcacaaagc gcgcaccggt gagacccttt ccttcaccgg cttcctggtc tcttgcctgg
    30181 cccgcgcggt ggatgaggac aaggccgtgc aggcctacct gaaaggccgt aaacagctcg
    30241 tcctgtttga cgacgtaaac gtcgggttga tggtcgaagg gaagattgga gaaaagcgcg
    30301 ccttgatggg gcatgtcatc cggggcgcga accacaagac ataccgggag atccaccagg
    30361 agatccgctc ggtacagtcc gaaccggtgc cgccgagcag aggcatgccc acctggttcc
    30421 gttctgccat gctgcttccg tggccactgt ccaggctgat caaggctctg ctcggtatgg
    30481 ccatgcgtcg tgacccgacg attcccgtgt cgatggcagg cacggtcggc ataacctctg
    30541 ttggtatgtt tggcgggagt catagcggct gggggctaac tcccacatcc caatcgctcg
    30601 ccctggtggt ggggggtact gcctggaagc cagccgtcgt ggcggggcgg atcgagccgc
    30661 gcgagatcct caacctgacg gtggcgttcg accacgatat tgtcgacggc gccccggcgg
    30721 cccggtttac gcggagattg gtagagctga tcgagagcgg atatgggctg gacgagattg
    30781 agccggctaa ccatcaatac gtgagtaatc ccgcgtgact ttctcggtcc tgttgatcgt
    30841 ttgacaagac gcaagtccac gtgtgtggcg attgctactc tagcgtgggg gaaccgaaaa
    30901 ggaagatcag gatggctggg cgtgggagcc cagcctgctg gtttcagata ggcacttaac
    30961 gcccaaagcc cgcaccggct caagccggcg cgaggcgcga cgtgtccgtt cggatctgga
    31021 caaagagcgg ttcggcttgt tccgcgtacc agataaattc gacattgatc ggccggcccc
    31081 agtattcggc catggtccgg acatagctgg cgagtcgctg gacttcatcg gcggtgaata
    31141 ccgggctgga tggcgtcggc gccctgattt ctccgcctgc cgcaccggag atcgtaatgt
    31201 gtggcggcgg catgtggcgc tcggttacgt tgacatcttg cattgcataa aggtcaccgc
    31261 cgtgaccctc gacgacgccc cgtcccgcgc cgaacatcgc ctggatgagg acgcccggca
    31321 ggttgggcac gggcgaatga ctgaaggcca cccccgaccg ttcctgcgcc gtgatcggct
    31381 tcatgggctg cagaatgacg gccatcttcg gccaagtcgt gcccgagtgc gcgcgataac
    31441 tgagcacctc gcgcttgaag gccgaagccc acacttgacg gatcgcctgc gtcaagctcg
    31501 cgaggtctga aggcacggac agcatcgaga aaaactgtcc cgcgtagcta tgcgcatccc
    31561 gatcttcgac cgtggccgac gatcggacgg cccattgggg gtggtccagg gtcggcagcc
    31621 ggagcagcag ggcaagcact gcctcaggca aaggtgactc ctccagtgca ttcacgatgg
    31681 cctgacgctg tggctccacg tcgtcgagca tgcgctgtgc cgattcccag tccggcgtcg
    31741 aggcggtggc ctgatcgatt tcacgcacca ctgcggccac gccggcgtgc tcgaccatcc
    31801 gatcgaacac agtcgtgctg agcacccagg tcggcggcgt gcgaaagccg ttgacctgca
    31861 gccagtacag gccgctgccc ttaccgccgg catgcctgat cggcggaatg atgccatcca
    31921 atgtgatcga gtcgggtgtg aataacatga gctgactccc cgctacgtat ccacgatgtc
    31981 gaccgcgccg gtcgccccat cgacgcgtag ccgctggcca tcgcgcaccc agcgggtcgc
    32041 ctcgggcaga ccgacgacgg ccggcaggcc attttcgcgg gcgacgatcg ccgcgtgcga
    32101 gatgacgcca cctagctcgg tgacgaccgc actgagccgg cgaaacaggc tcgaccaggc
    32161 cggatcggtc aaccgcgtga ccagcaccac cggtccgcga aggcgcagca gccgctgccc
    32221 ggcatccagc ggatcattgg tcactatggc gatgccttcg gccgggccgc tgccggcgct
    32281 ggccggaatg ccctcccgat gcgtgacgaa aggcttgagc gctgcgcgct cgcgttcctg
    32341 caccggcgtc catctgccgg tgtcctgatc gtaccatatc aattccggtg ggggcagttg
    32401 agatgaatac aattcgtact gggcacggcg cgcgtcgagc gtttcccgct tcacccgttt
    32461 ctcaccgcgg gcgagcacga aggccagaaa ctcatccatt gaataaaaca tgaggatatc
    32521 gtcagactca tccgggaaga aatagcgggc caggcggcgc gcggccagcc cataggcggc
    32581 ctgacgcagc gccaagttgc ttttggaagc ttcgcgccag gcaatgagct gctggcccca
    32641 tcgcagcacg cgccgccaca cggcgcgcga gagcggattc atccggttca agttgtcgag
    32701 cgcgatctgg cgtgcggctt cggcacgcgc atctggcgat tcgctttccg caagctgccc
    32761 gccgatcgcc agcaggatat gctctgggtg atcggccagg cgctcggttt caaaatccgc
    32821 ctcgtgccgg aacccgcgat ggccatagac tatcaagaag ttctcgatgc ccgcgaccaa
    32881 gggggcggcc tcgggtcttg cgcgataccg ctcgagatcc agcacgccgt cgcgcagcat
    32941 ctggcaaacc tgttctccac aggcttctgc cgtctcgcgt aacgcccaaa tgtgccggcc
    33001 gatttgtgac gtggcggtgg actggccggc gaacaagccc agcagcccgg gtacacgctg
    33061 gcgcaatatg gtatccaccg agacgacgat cagcgagatg acgacatggg cgcgatctgt
    33121 accccgcgag cgcttgtaca ggcctggctc aaataaggcc caggccggcg cgaggtcagc
    33181 gccggtgcgc agttgccagt acaggcggtg cagatcgtga tccacaccag gcagcgtgtg
    33241 gcgatagaaa tgcgttgccc agcgatacac gttccgcaag cgcggcagca agaccatctg
    33301 ttgtcccagg ctgggacgac tcagattctt gaccatctcc ggcggcacgc cgattgacga
    33361 cgtatcgaac ggcacgactt tggaaatggt gcgcgcaaag tattccatat tcaggtacag
    33421 gcggccctga acggactggg tagccttcac cgtccggtca aagcgagaca ggtcgccccc
    33481 cagttgttcc aggacgaaat caaactgagc gtccaggccc ttctcggtca tcgtcgccgt
    33541 cgccgggcgc gccaggcccg tcgtttcttg cgtgttgtgt ccgctgagca gcagttcggt
    33601 catccgaccc tcctcgccct ttcgagaaat cctggaattc ggcggccgtc cggtgcggtg
    33661 tacatggccg ggccgatttc gtcacggtat agatcgccgg cgtgcgcctc gggccgaacg
    33721 gcttcctggc cgggcggccc caggtgcacg acgaacggcg acgtgacacg ctcaacctgc
    33781 catacgccgg cgtcagtctg atcgtcggcg atgcgccacc cgtttccatc agccgatacg
    33841 gccggcgccc ggaaagcttc gcgctggcca tccggtaaga agaagtggcc aaagtggtgc
    33901 tgctgtccgt cgatgtggat cacagccaga tacatggagc gaggcagact cgtcacgatc
    33961 cagtcttcgg cgatgtgcat ctgtcgccgg ccccaacttc gatcgcgcga gcacagaaac
    34021 tcgccgccgt atcgctggcc gtccaatctg aacgatcccg acatgacgcc atcttgctgg
    34081 acctgttgat aaggcccgaa ctgatagcga gtcggcgggc catcggcgtg aaactgaaaa
    34141 tcgagtgcaa agctggcgct atggctggag gcaatgtcgg tcagcggacc gtcgaagata
    34201 cagctccact tcgcccacgg ctccaggcac gccagcttca cggtaggtcc ggaagcatcg
    34261 tgggacggtg tcggtccgcc cgacaaagga c
//
LOCUS       DBNHXB010000070        17929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1355708, whole genome shotgun sequence.
ACCESSION   DBNHXB010000070 DBNHXB010000000
VERSION     DBNHXB010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 17929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17929
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1355708"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..311
                     /locus_tag="AC162G_02710"
     CDS             156..311
                     /locus_tag="AC162G_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074758.1"
                     /translation="MRLTRTLGMLLLAIWLIVTGLVPLLSLSFSGLGTLMAILAIAAG
                     ALILIGR"
     gene            748..1176
                     /locus_tag="AC162G_02715"
     CDS             748..1176
                     /locus_tag="AC162G_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5074759.1"
                     /translation="MARTRVVLVVVHDAVRASLRGLLNGTMDIEVIGEARDVVEALRL
                     VEELKPDVLVWDIDTSRLNGFEVTRRLHAAQSPVHILALSAYEDTHFILGFLAEGVTG
                     YLVKDDAPKTIVEAVRSVSRGEKWLSPRAAAKVARKQDVL"
     gene            1195..1899
                     /locus_tag="AC162G_02720"
     CDS             1195..1899
                     /locus_tag="AC162G_02720"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR5074760.1"
                     /translation="MFPLSDEPRRRGSGPALVTLGLIAINVLVLVYEVTLGAEVEGLI
                     RTYGAVPREILTGQDVPPPGPTPLWIQLITSMFIHGGWLHLIVNMAYLRVFGDDIEDA
                     MGAVRYLLFYLAAGITASLTHIVLSGPGDTTPSVGASGAIAGVLGAYLVWFPRRQVHV
                     LLPILFFFTARVSALLMLGMWFLLQFLNGIAAITEGTAQTGGVAVWAHIGGFVAGAIV
                     AVLLRGSFRPRRHVYA"
     gene            2235..2516
                     /locus_tag="AC162G_02725"
     CDS             2235..2516
                     /locus_tag="AC162G_02725"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR5074761.1"
                     /translation="MHQVDSTLATRVMEALLRDPRTWEAAIDVSSAGGALTLSGRVSS
                     EGIRQAAEDIARRQKGVITVINDLDVASHDERSGALLDDDETVPIKVKA"
     gene            2540..2914
                     /locus_tag="AC162G_02730"
     CDS             2540..2914
                     /locus_tag="AC162G_02730"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR5074762.1"
                     /translation="MKVTAQPYPISILGRKRFALRLTHWIWLILGILEGLIGLRVLLK
                     LLGANPAAPFAQFIYSLTDPFLFPFFGLTEAPTAGNFVLETYSLVAMFVYLLLTWTVL
                     KVIALIAYPPDEGSTVSALDQH"
     gene            3023..3271
                     /locus_tag="AC162G_02735"
     CDS             3023..3271
                     /locus_tag="AC162G_02735"
                     /inference="COORDINATES: protein motif:HMM:NF016141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR5074763.1"
                     /translation="MTAEQVLVSVFIGGIAGAVADAVVGGVRVGCLGAIIVGILGGLI
                     GSWLFAQLGIAVGTGLLGSIVTAFVGAVIILVILRAVR"
     gene            3401..3832
                     /locus_tag="AC162G_02740"
     CDS             3401..3832
                     /locus_tag="AC162G_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5074764.1"
                     /translation="MIRRNVATILIALVTVLALSACTGAGGVEVTPGGEVTPAPSAST
                     ISVEATEFAFTPSTFTAQVGEELTFEITNNGTLEHNFVVLDPAGDELARTSIAVGAVE
                     SVKIVPVEGGAYTIVCDMEGHQEAGMEAALTVQGVALRQEP"
     gene            3829..4077
                     /locus_tag="AC162G_02745"
     CDS             3829..4077
                     /locus_tag="AC162G_02745"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR5074765.1"
                     /translation="MTWIDSEITARVIAALLNDPHASASLIEVNHERGVVTLSGTVLS
                     EEARRMAEEVVRRQRGVRRIINNLCVAPRQWKRAALRA"
     gene            complement(4209..4739)
                     /locus_tag="AC162G_02750"
     CDS             complement(4209..4739)
                     /locus_tag="AC162G_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074766.1"
                     /translation="MSQVKPDSPRVAAERAERSVPEWVYPYRAGVVGGALGGLVMVAV
                     ALAYGIWSGRGVWLPVNLIGATIVRDLQSASIDTLSQFNLAALIAGLVLHFTLSIGLG
                     FVFALLLPTFPGPPIIWSLTVGPLLWAIASLLALPIINPIMAENVEVSSFFIAHLAYG
                     LVLGWYVRRQPKIHVS"
     gene            5030..7039
                     /locus_tag="AC162G_02755"
     CDS             5030..7039
                     /locus_tag="AC162G_02755"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR5074767.1"
                     /translation="MLAWIRPRTGTILVSVALAVMGLALTAGVVLAQDAEPPAYREFP
                     LIGSRVAVWIAAEVHLMFAAFMLGVPMFALVVEYVGMRTRERRYDDLAREFTKLILVA
                     ASTTAAFGALLTFLLFALYPNYMSRLTGVFLPTMILYPLLFLGEIFSLYLYWYSWDRL
                     NTPGGKRWHLLIGLMLNVFGTVLMFIANAWLTFAISPSSGGQSLYDSAGNFTGTLGQA
                     INNATWWPINIHRIIANVAFGGAVVGMYAAMRFLNARAQADRAHYDWMGYVGNFIAVI
                     GLIPLPFAGYYLGREVYAFSQQMGVTMMGGFLSWLWILQAMLIALIFLSANYYLWIGM
                     GRIEGGQRYAGHIKWLLATLTLCVLVWATPHNPVVTPAEQALIGGAFHPLLGVLGVMS
                     AKNTAVNIMILTTFLSFLFYRRAGKGDPLPFASQGRSARIALVVAAGICVLLAAWPAL
                     AVFREGLTEFSRVARVGLMVLHGLVIVAAAVLTFRDRGKLGQWLILGSAAFVVVFFGV
                     YGYVVEATIRIGFSVYQVLAVLTALISVTAIDVFLFRGAPSLGAIQWGRMPVRSQYIL
                     ILLAVTFTWLMGLMGYARNAIRQHWHVYQVVQDTSPDAFSPTLGFAALVISGCVLLFL
                     ALIAFIFWLGRLGEQKAAGESPPAEAQVVSAPGALEPGPALASTK"
     gene            7036..7821
                     /locus_tag="AC162G_02760"
     CDS             7036..7821
                     /locus_tag="AC162G_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5074768.1"
                     /translation="MKTTLSVSIFVIAVVALIAGFATLIPQLESPAPETLDISGELSG
                     TELAELGRSVFESAEAGCLTCHGLGREGLRAPDLAGIGSRAAERIPGKSTEEYIRESL
                     VDPCASVVEGYDCIMPKTLLQTLGPAKITALIAFLQSQGGEVTVRLTAEEAAGGTATS
                     GGSTGGVAGATAEEIVAGAGCVACHQIDAVGAAGQVGPNLSQVGARLALEEIRQSILT
                     PDAVIAEKCPSGACPPGVMPKDFGNRLSAIQLETLVTFLSGLK"
     gene            7921..8727
                     /locus_tag="AC162G_02765"
     CDS             7921..8727
                     /locus_tag="AC162G_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5074769.1"
                     /translation="MPSLASLLKHKLTQFILVLVVGYLVFQFGIPALSRALTGVAAPV
                     PSHLLWTIFMPILGLVMLLYVSANEAAWREFKAPLRTLLVERERRQVVTLRRAILVAL
                     PLLVGLAVFLSLQSGVSPPAELRSVHPAPPASITFDGKLIDLQTTINPFRDASGGSDP
                     GALAEGKAIYAQHCVFCHGDALSGDGLFADSLRPRPANFTDPGTIAQLQESFLFWRIA
                     KGGPGLPPEGKGWNSAMPAWEGTLSEDEIWKVILYLYDATNQHPREAGLE"
     gene            10083..11492
                     /locus_tag="AC162G_02770"
     CDS             10083..11492
                     /locus_tag="AC162G_02770"
                     /inference="COORDINATES: protein motif:HMM:NF021010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5074770.1"
                     /translation="MDSKAIYTQRCEVCHGAEGDGNGPAAERMSPRPRDFTTGAFKIR
                     TTPSDSPPTDADLIDVISNGMPGSTMPAWKDVLSGDEIAAVASYVKTFSDAFEGEAPE
                     SIRTGNRASADAASIARGAEVYREVQCFKCHGDEGRGDGPSALTLQDEFGHEIFPADL
                     TQAWLFRGGGTADDIYKRIVTGMTGSPMPSFADALVDADGNPDEQKRWDLVNYVDSLS
                     PDTWSGPPDALVARRIEGDIPTDPNAPEWQSATPYYIPLVGQIMREPRNFTPSIAGVW
                     VRPLYNANEMALLVQWNDRQQNMGVEGESTDEIAVQFPAATTERDERPYFVFGDAGYP
                     VNLWTWAAGASTAIEKIGRGSDAIAPQPTQNLSAAGSYNAGQYSVIFQRALSTGDVDD
                     IVLTPDTFVPIAFAASDGWRGEQLGAGAISAWYPLYLEQPTPPARYLWVPIAMIATVA
                     VEGLVVWLVRRDAQRIAGS"
     gene            11590..11973
                     /locus_tag="AC162G_02775"
     CDS             11590..11973
                     /locus_tag="AC162G_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074771.1"
                     /translation="MAQWYVQLVFKGDTLLGEGVADLGEVVDIALPNGGERYTTVIVS
                     GYALYGQLRGYYSLGIAHVTVDPGSKRVAYVSETSALHKEPVGSLTLAQRAAIRDWLM
                     RLNPAAWENSTEVFRRELERVTENG"
     gene            12031..12816
                     /locus_tag="AC162G_02780"
     CDS             12031..12816
                     /locus_tag="AC162G_02780"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR5074772.1"
                     /translation="MQLYKLLVALIAVGVFIAACGGGGAPTAPPSGGTPEGGGEEVTS
                     PVDPATAGSVAGRVNFTGTAPEPEPILMEAEPECQAQYPDGAFTETVVVNDNGTLQNV
                     FVYVKEGLGDLKVPTPQEAVLLDQQGCRYAPHVLGVQVSQDITIRNSDGILHNIHPVP
                     TLNKSFNLGQPVKMDSTRQFDQVEVMIPIQCDVHDWMIGYAGVVDHPYFAVTGADGTF
                     ALPDLPPGTYTIAAWHELYGEQTMQVTVGEKETKEIEFTFEGK"
     gene            12911..13615
                     /gene="coxB"
                     /locus_tag="AC162G_02785"
     CDS             12911..13615
                     /gene="coxB"
                     /locus_tag="AC162G_02785"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR5074773.1"
                     /translation="MGGLIPQASTFAASIDSLFVVVLIVTGVAFVLVEGILIYFLVRY
                     RNRTGRKASYVHGSQRLELAWTVGTGIAFFALAIYQYNAWIQIKAALPDRSAAQLVGI
                     SSNQFEWEATYPADDGQLGTADDIKAPINVVHFPVGRPVIVRLESVDVIHSFFVPELR
                     VKQDAVPGQPIEFWFEATQSGQYEIACAELCGLGHYRMRGQVTVESQADFDAWLTEIK
                     AKRGAVGVPLSSSELP"
     gene            13666..15426
                     /locus_tag="AC162G_02790"
     CDS             13666..15426
                     /locus_tag="AC162G_02790"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR5074774.1"
                     /translation="MTSDKELPFIRRYVFSTDHKVIGKQFLFTALAFFTIGGLLALLI
                     RTQLAWPWQEVPFFGRLLYGEEAGNILAPERYPALVTMHGTIMIFFFIIPVLSGAFGN
                     FLIPLMIGARDMAFPRLNMASYWLMVPAFLVMVASFFVEGGTAASGWTSYVPISARPD
                     MIEGSGMGQTLWIISLILVGTSSLLGAINYITTIVTLRAPGMSMFRLPMTVWALFITA
                     ILVLLATPVLTSALILLLLDRSAGTHFFAANSGGQVILWQHLFWFYSHPAVYIMILPA
                     MGMASDIISCFARKPLFGYKPMIFSIMAIAVLGFRVWGHHMFQSCMNPLLGTTFMTAT
                     MMIAVPSAIKTFNWLGTLWQGNLRLSAPMLNALAFVSTFVIGGLSGVFAAATPVDIFI
                     HDTYWIVGHIHYVLFGGSLFGVFAAIQYWFPKMFGRQMNDGWGKIHFVLTFIAFNCVF
                     FPMFILGANGMPRRIADAYQYQLLAPLQSLNQFMTVSAYVLGAAQLILAANLAWGLFR
                     GKKAVDNPWEANSLEWQTGSPPPLENFHAIPTVYRGPYEYSSPEVQEDWLPQNRYLDG
                     AKPQKKMPAEVGLAAGAAGE"
     gene            15455..16048
                     /locus_tag="AC162G_02795"
     CDS             15455..16048
                     /locus_tag="AC162G_02795"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR5074775.1"
                     /translation="MSHTTASEPHATPIWQGIPTTKFAMWLFLASEVMFFAGLIGAYI
                     VLRSGAPEWPVVSEILNVPLVAGNTFILIVSSVTMVRAFAAIEDGNPQGMRRMLIATA
                     ALGTIFLSIQAVEWSALLSEGTSPSTSVFGSVFFTLTGFHGLHVLGGVLTLVVVIVNA
                     FRGAFTQASHGGVELMGLYWHFVDVVWIFLFTILYLI"
     gene            16130..16417
                     /locus_tag="AC162G_02800"
     CDS             16130..16417
                     /locus_tag="AC162G_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HIR5074776.1"
                     /translation="MAETSIVHRRPNYILVFVFLGLLTAVEVGVAFTGLPKPVQVPLL
                     VALAIAKATLVGMYYMHLRFEGRVLRFIAVGPLLLAIILTLPPLVDSISHH"
     gene            16513..17058
                     /locus_tag="AC162G_02805"
     CDS             16513..17058
                     /locus_tag="AC162G_02805"
                     /inference="COORDINATES: protein motif:HMM:NF016153.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HIR5074777.1"
                     /translation="MVSIRTHLRVPKTRAGWLMGGISALVVLSVAVLLAAPRGETPGR
                     FDVSALPRLNAALNGASAILLVAAYFFIRHRRVKAHRACMLSAFAVSALFLMSYVIYH
                     IFAGSVPFAGPGWIRPIYFAVLISHMALAALVLPLALTTLYRAWRGRFVRHRQMARWT
                     LPIWLYVSVSGVAVYLMLYHL"
     gene            17144..>17929
                     /locus_tag="AC162G_02810"
     CDS             17144..>17929
                     /locus_tag="AC162G_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5074778.1"
                     /translation="MTIVTTPVTDSAPARAWQSGPLYRKILIPTDNSELCHAGIDWAI
                     EFARAWDATLVGNHVYAARLHDDRFRQLEIGLPARFQTDEEIARQRKIHDKLIEQGLI
                     LISDSFLDVLNKRGEENNLKIERKLMEGIHFEQLAKDVNSGDYDLVVMGGHGIGQVKR
                     SQLGSVTSRVARLITADMLVMKQKERSLSGGKILVCVDSSAYCFVALRKALRLAQTFG
                     ATVHVVGAFDPFYHYAAFNNIRYVLSYQAGKVFKFEEQEELHNN"
BASE COUNT         3360 a         5287 c         5569 g         3713 t
ORIGIN      
        1 tcggatcggc gttgttcgtc gccgtgattg gcttcctggg gcgaggtcgc ctgtagccct
       61 cggccaatcc gagcgggcat gtaacaggcg aacggcttta tcaaccgctg tgatgcggag
      121 aaaccactat gcttcggaga agaaggagtg ttcccatgag actgaccagg actttgggca
      181 tgctgctgct cgctatctgg ctcatcgtga ccggacttgt ccctctgctc agcctaagct
      241 tttcgggctt aggcacgctg atggctattc tggccatcgc ggccggggcc ttgatcctga
      301 ttgggcgata ggccgtctgc ctcgtcgtcg atcaacgtgc ctgcggctca tcgctcactg
      361 gagccgctac gccgcctagc ccgctgctac ctgcgcctcg tggagcgcct ctcgcttcgg
      421 gggcctccgt gggcgcaccc ccactacgcg agggcaggtc agttgcccgc cgcgcgggat
      481 tcagagggcc atgcctgttg gcgtggccct tggataattg cgctttgcct ccttccggac
      541 ctgacacacc tgaagtgaga gtacatgggg acaaccctat cagataggga aagccctacc
      601 tcataggtcg gggactgccg tatatggaaa aggaacatcc cccattgtcg tatcgtgctc
      661 aggtgtttat agtaatagat aaggaagccc ccatgaactg ctgtccctga taggcgagaa
      721 gacggtaaag gatcgacaag caaacacatg gcgagaacgc gtgtcgtact ggtcgttgtt
      781 catgatgcgg ttcgagcgag tctccgtggc ctcttgaacg ggacaatgga catcgaggtg
      841 atcggagaag cccgcgacgt cgtcgaagca ctccgccttg tcgaggaatt aaagccagat
      901 gtgctggtat gggatatcga tacgtctcgc ctgaacggct ttgaagtgac ccggcgcctg
      961 cacgcggcgc aatcgcctgt ccacatcctg gccttgagcg cttatgagga cacgcacttc
     1021 atcctgggat ttctggcaga gggagtcaca ggatatcttg tcaaagatga cgcgcctaag
     1081 acgatcgtcg aggccgtgcg cagcgtatcg cgcggagaga agtggctcag tccacgggct
     1141 gcagccaagg tcgcgcgaaa acaggatgtc ctgtgatcga ctgggaggac gagtgtgttt
     1201 ccacttagtg atgaacctcg aaggaggggc agcggcccgg ctctcgtgac gctgggcttg
     1261 atcgcgatca acgtgctggt ccttgtgtac gaggtgacgt tgggcgcgga agtcgagggt
     1321 ctcatccgca cttatggagc ggtgccgcgc gagattctca ccgggcagga tgtgcccccg
     1381 cccgggccga ccccgctgtg gattcaactg attacctcca tgttcatcca tggcggctgg
     1441 ctgcacctga tcgtcaacat ggcctatctg cgagtgttcg gggacgacat tgaagacgcg
     1501 atgggcgccg tgaggtatct gctgttctat ctggccgccg gcatcacggc ctcgctgacg
     1561 cacatcgtct taagcggccc cggcgacaca acgccgagcg tgggcgccag tggagccatt
     1621 gccggcgtgt tgggcgctta cctggtctgg tttccacggc gacaggtgca tgtgctcttg
     1681 cccatcctgt tttttttcac ggcgcgcgta agcgcgctgc tgatgttggg tatgtggttt
     1741 ctcctgcagt ttctcaacgg gattgcggcc atcaccgagg gcaccgcgca gaccggcggc
     1801 gtggccgtct gggcgcacat tggtgggttt gtggccgggg cgatcgtggc agtgttgctg
     1861 cgcggtagct tccggccccg gcggcatgtc tacgcataaa cgcgtcacag ggtagggatt
     1921 tccctaccgt gatccggcat atccctcatc ccgggaaggc gaatccccca ttgattgatc
     1981 cctcgaaagg acgtatcctt gacgtgcagg aacaatcgaa cacaccgtcg ggaatgtcca
     2041 agcatcgaga tggttcgact ttgttcctgc acctactccg agccggttaa gaatcccttg
     2101 accggctcgg accaacccaa accatcccag aatcagcgac cacttcgttt ctgggtataa
     2161 cgcaatcgga ggccagcgct atgagtaaac ggcctgataa ccgaaagaga cgcaagaacg
     2221 aaggagctag aaccatgcac caggttgatt ctaccttggc cacccgggtg atggaagcac
     2281 tgctcagaga tcctcgcacg tgggaagcag ccatcgacgt gagtagcgca ggcggcgcct
     2341 tgacgttgtc gggacgggtt tcctcggaag gcatccggca ggccgctgag gacatcgccc
     2401 gccgacagaa gggtgtgatc accgtgatca acgatctcga tgttgcatcg cacgatgagc
     2461 gatcgggcgc gctgcttgat gatgacgaaa cggtacccat caaggtgaaa gcataacgat
     2521 ggccacacag cgtgagagca tgaaggtgac cgcacaaccc tatcccatca gcatcctggg
     2581 gcgtaaacgc ttcgccctga gactcacgca ttggatctgg ttgatcctcg gcatcctgga
     2641 gggtcttatc gggctgcgcg tgttactgaa gttgttgggc gccaatccag ccgcgccgtt
     2701 tgcccaattc atctatagct tgactgatcc gttcctgttc ccgtttttcg ggctgacgga
     2761 ggcgcctacc gccggcaact tcgtgctgga gacctattcg ctcgtcgcca tgttcgtcta
     2821 tctgctgctg acctggaccg tcttgaaggt catcgccttg attgcctatc cgcctgatga
     2881 gggaagcact gtcagcgctt tggatcaaca ctgatcccgg acgggcgtgt gaggtgatgc
     2941 gactggcaat cgatgcctca ccagggccgg ttcaagccaa tgcgcacgat agacaccgtt
     3001 tatccagagg agggattagg ccatgacagc agagcaggta ctcgtctcag tgttcatcgg
     3061 tggcatcgcc ggggcagtgg ccgacgcggt ggtcggcggc gttcgggtgg gctgtttagg
     3121 cgccattatt gtgggtatcc tgggcggtct gatcgggtcc tggttgtttg cccaattagg
     3181 gatcgccgtg ggaacaggtc ttttaggcag cattgtcact gcattcgttg gcgccgtgat
     3241 catactggtg attttgcgcg cagtgcgctg accacgctgc ctgcgtgtgt ccgtcacgtt
     3301 tcaagacaca gattgcacta cctagcgctt acagggtatg gacgcaacga ggtatacgtg
     3361 caatccggtt cagaacatcg gtcaagagag gaggtatccg atgattcgcc gtaatgtggc
     3421 aaccattctc attgcgctgg tcacggtact ggcgctgagc gcgtgcaccg gagcaggagg
     3481 agttgaagtg acgccaggag gtgaagtgac gccggctccg tcggctagca cgatctcggt
     3541 cgaggcgacc gaattcgcct ttacgcccag cacctttacc gcccaagtcg gcgaagagct
     3601 caccttcgag atcacgaaca acggcaccct tgagcacaac tttgtcgtgc tcgatccagc
     3661 cggcgacgag ctggcccgca ccagcattgc cgttggggcg gttgagagcg tgaagatcgt
     3721 gccggtggaa ggcggcgcct acaccatcgt gtgcgatatg gagggccacc aagaggccgg
     3781 catggaagcc gctttgaccg ttcaaggcgt ggccctgagg caagaaccat gacctggatc
     3841 gactccgaga taacagcgcg tgtgattgcg gctctgctga atgatccaca cgccagcgcg
     3901 tctctgatag aagtcaacca cgaacgtggc gtggtgactc tgtcgggaac cgtgctgtcg
     3961 gaggaagctc gccgcatggc agaagaagtc gtgcgccggc agagaggtgt ccggcgcatc
     4021 atcaataacc tgtgcgtcgc gccacgtcag tggaagcgcg cggctctacg agcctgagtc
     4081 gcggcttgaa gccgcactca cgcagggacg cgattccgca ctcacgttgg agacgtgatg
     4141 ccagtgttgg gtggaatcga tggagagggg cgtcgaggtg ttagccggcg cccctttgtc
     4201 ttgtctcact aactcacgtg aatctttggc tgacgcctca cgtaccaacc cagaaccagc
     4261 ccataggcga ggtgcgcgat gaaaaaggac gacacctcga cgttctcggc catgatcggg
     4321 ttgattatgg gcagggcgag caggctggcg atcgcccaca gcagcgggcc gacggtgagt
     4381 gaccagatga taggcggacc gggaaacgtc ggcagcagga gcgcgaaaac aaagcccaat
     4441 cctatcgaga gtgtgaaatg cagcacgagt ccggcgatca gcgcggccag gttgaactgc
     4501 gacagcgtgt cgattgatgc gctctgcaag tcgcgcacga tcgtggcgcc gatcaggtta
     4561 accggcagcc acacgccgcg cccgctccag atgccgtagg ccagcgcgac ggcaaccatg
     4621 accagccccc ccagcgcgcc gccgaccacg cccgccctgt atggatatac ccattctggc
     4681 acggatcgtt cggcgcgttc cgccgccacc cgtggcgagt caggcttgac ctgagacatg
     4741 atccccctcc catttccttg gctattagat attatagacc ctttgtcaac aaaattcgcg
     4801 cttgacttta cctgcgaatt tgatacacta agcccatgtc tcagacctac ccgaaaaccc
     4861 tgtaacccgg agccttgtgc tcgccgccca cgaagggcta cactgtgtat tcggataggc
     4921 tcttagttga taaccgcata tcgactgatt aagtgagccg cggtccccgc ctgtcagcat
     4981 gaccggcagg ctgagcttag caatggcaca atagccaagg gagggtgcct tgctcgcttg
     5041 gatccgtcca cgaaccggta cgatcctcgt cagtgtggcg ctggctgtca tggggcttgc
     5101 gctgacggcg ggtgttgtgc ttgcgcaaga cgctgagccg cccgcctatc gtgaatttcc
     5161 cctcatcggc agccgggtcg ccgtctggat cgcagccgaa gtccacttga tgttcgccgc
     5221 cttcatgctc ggcgtgccga tgttcgccct ggtcgtcgag tacgtcggca tgcgcacgcg
     5281 agagcggcgc tacgacgatc tggcccgcga attcaccaag ctcatcctcg tcgccgcctc
     5341 gacgacggcc gccttcgggg cgctcctcac gtttctcctc tttgcgctgt atcccaacta
     5401 catgagtcgg ctgaccggcg tcttcctgcc gactatgatt ctctacccgc tgctcttcct
     5461 cggtgaaatc ttctcgctct atctgtactg gtattcgtgg gatcggctga acacgcctgg
     5521 aggcaaacgc tggcatttgc tgatcggcct tatgctgaac gtgttcggca cggttctgat
     5581 gttcatcgcc aacgcctggc tgacgtttgc catcagtccc tcgtccgggg gccagtcgct
     5641 gtacgattcg gcgggcaatt tcacgggcac gcttgggcag gcgattaata acgcgacctg
     5701 gtggccgatc aacatccacc ggattatcgc caacgtcgcc ttcggcggcg cggtcgtcgg
     5761 catgtacgcg gccatgcgct tcctgaatgc gcgcgcgcag gccgaccgcg ctcactacga
     5821 ctggatgggc tacgtcggca acttcattgc cgtgatcggg ttgatcccat tgcccttcgc
     5881 cggatattat cttggccggg aagtgtacgc ctttagccag caaatgggcg tgacgatgat
     5941 gggcgggttc ctgtcgtggc tgtggattct tcaggcaatg ctcatcgcgt tgattttcct
     6001 gtcggccaat tactacctgt ggatcggcat gggacgcatc gagggcggcc agcgctacgc
     6061 aggccacatc aagtggctgc tcgccacact gaccctgtgc gtgctggtgt gggccacgcc
     6121 gcacaaccca gtcgtcacgc ctgccgagca ggcgttgatc ggcggcgcat tccatccgct
     6181 gctgggcgtg ctcggcgtga tgtcagccaa gaacaccgcc gtgaacatca tgatcctgac
     6241 gactttcctc agcttcctgt tctatcgacg cgcgggcaaa ggcgatccac ttcccttcgc
     6301 cagccagggt cgatcggcgc gcattgccct cgtcgtcgcc gccgggattt gtgtgttgct
     6361 ggcggcgtgg ccggctctcg ctgtgtttcg agaaggattg accgagttct ccagagtcgc
     6421 gcgtgttggc ctgatggtct tgcacggtct ggtcatcgtc gccgcagcgg ttctgacctt
     6481 tcgcgaccgg ggcaagctcg gccaatggct gatcctggga tcggctgcct ttgtcgtcgt
     6541 cttcttcggt gtgtacggct acgttgtcga ggcgaccatc cgcattggct tttcggtgta
     6601 tcaagttttg gcggtgctaa ccgcgctcat ctcggtgacg gcgatcgacg tgttcctgtt
     6661 tcgcggcgcg ccgagcctgg gcgcgattca atggggccga atgcccgtgc gttcccagta
     6721 catcctcatt ctgctggccg tcacatttac gtggctgatg ggcctgatgg gctacgcccg
     6781 caacgcgatc cggcagcact ggcacgtcta ccaggtggtg caggacacgt cgccggatgc
     6841 cttctcgccg acgctcggtt tcgccgcgct ggtgatttcg gggtgcgtgc tgctgtttct
     6901 ggcgttgatc gcgttcatct tctggctcgg caggcttggc gaacagaaag cagccggcga
     6961 atcgccgccg gccgaggcgc aagtcgtctc cgcgcccggc gcgctcgaac cggggccagc
     7021 cctcgcaagc acgaaatgaa aacgacactg tccgtttcga tcttcgtcat cgccgtagtc
     7081 gccttgatcg cgggctttgc cacgttgatc ccgcagctcg agtcgcctgc gcccgaaacg
     7141 ctcgacatct ccggcgaatt gtctggcacg gaactggctg agttgggccg atccgtgttc
     7201 gagagcgccg aggcgggctg tctcacctgc catgggcttg gccgagaagg attgcgcgcg
     7261 cccgatctgg caggcattgg cagccgggca gccgaacgca tccctggcaa atcgaccgaa
     7321 gaatacatcc gcgagtcgct cgtcgatccg tgcgcctccg tcgtcgaggg ctacgactgt
     7381 attatgccta agaccttgtt gcagacgctc ggcccggcca agatcaccgc gctgattgcg
     7441 ttcctgcaaa gccagggcgg tgaggtcacc gtccgcctga cggccgagga agccgccggc
     7501 ggaacagcca cgagtggcgg aagcaccggc ggtgtagccg gcgcgactgc cgaagagatt
     7561 gtcgccggtg caggctgcgt cgcgtgccat cagatcgacg cggttggcgc ggcgggccag
     7621 gtcgggccga acctgtcgca ggtcggcgcg cggctcgcgc tggaagagat tcgccagtcg
     7681 atcctcacgc ccgacgccgt catcgccgag aagtgcccga gcggtgcgtg tccaccgggc
     7741 gtgatgccga aagacttcgg caatcgactg agcgcgattc aactggaaac gctggtgacg
     7801 ttcttgagtg gtcttaaata gagagaggta attagggatt ggtaatgggt agacgtgagc
     7861 cgctgccaat ctccagcctc caataaccaa taaccaatta ccaagaaccg ggaaatacct
     7921 gtgcccagtc tcgcttccct gttgaaacac aagctgaccc agttcatcct ggtgctcgtg
     7981 gttgggtacc tggtctttca attcggcatt ccggcgctgt cgcgcgcgct gaccggcgtc
     8041 gcagcgcccg ttccgtctca cttgctgtgg acgatcttca tgccgatact cgggctggtt
     8101 atgctgctgt acgtttcagc gaacgaggcg gcgtggcgcg aattcaaggc tccgttgcgg
     8161 acgctgctgg tcgaacgcga gcggcgccag gtcgtcacgc tgcgccgtgc catcctggtc
     8221 gctttgcctt tgctcgtcgg gctggctgtg ttcctctcgc tgcagtctgg cgtcagtccg
     8281 ccggccgagc tgcgctccgt ccacccggcg ccgccggcct cgattacgtt cgacggcaag
     8341 ctgatcgact tgcagacgac gatcaatccc ttccgcgacg cgagtggggg atctgaccca
     8401 ggcgcgctcg ccgagggcaa agccatctat gcgcagcatt gcgtcttctg ccatggcgac
     8461 gcgctgagcg gcgacgggct gttcgccgat tcgcttcgcc cgcgcccggc caacttcacc
     8521 gacccgggca cgatcgccca attgcaggag tcgttcttgt tctggcggat cgcgaaaggc
     8581 ggccctggcc tgccgcccga gggcaaaggg tggaacagcg caatgcctgc ctgggaaggg
     8641 acgctctcgg aagatgaaat ctggaaggtg atactctatt tgtacgacgc gacgaaccaa
     8701 catccgaggg aggcggggtt ggagtagcag ttttcgctct cgccggattt gcaacgcaag
     8761 tggaacggag ggaactgagg gaactcaggg aactccgtag cagtcttcgc tctcggcgga
     8821 tttgcggcgc aaagcggtac gacccaacgg tgggacgctt cgcacaaatc cggctggtgc
     8881 aaaggtcagg agtgacccaa ggggcattgt cccgtatttt gcgcgataac gctccgattt
     8941 ggggtattgt tccgcaggca gcgggtgaaa atgtagttag gcagccaagt gttggttcga
     9001 tgatttagat tagcactcaa ctgaaaggcg agaatccagt gagagagtat ccttcctgtg
     9061 attgctttga ccagtatgca gtgcaagagt ggtccgaact caagtttcat acagaaaagc
     9121 aagataccga aagtgatggt tggaagcacc tcttggatct cgtagaggaa gcagccgtgg
     9181 atggacgaga agaattcgct cccggacgtg aaatgactca agaagagtgg agccagattg
     9241 tgacgcttcc ccccacgatc tcgaaactga agtccgtcaa gcatctccta ttatacggaa
     9301 gctatctggt tcgcattcct ccagagatcg gggaaatgac gaatctgcaa gagttcacgc
     9361 cttacacctc atctcgatta cactggttcc cttatgaaat cacccgctgc aaggagctga
     9421 gaaacagtac agttagtaca cgtgctctct acggcaattt caaattcagg ccgccatttc
     9481 ctaaactcca accagacaga agttctacga agggactcga actagacaac ttgccaccgg
     9541 agatttgggg gacagagtca attactactt gcagtgtttg caaccgttcc ttggatgact
     9601 ctggcttata ccaagtatgg atatctctat ctgtggctac agatgttctt ccccttctcg
     9661 taaacgcgtg ttcagaacag tgtattcaca ggcttccgaa gtcagctgac gaatacgtac
     9721 aagggccgca caagggaggc cgggatatga atcaaccacc ctcactcatt gacgaaatag
     9781 ccgcaagggc aaagaaggtg tgagtatcaa gggagaaggc gacttcggct gcctaacaac
     9841 tcaagaaagc ggaccaccta ccgcgccggt tttcaaaggc tgttggcctg cgaggtagtg
     9901 tcttaaccaa agtgccttgc ccatccggtg gcggcttatc tcgaccgtta gcgcaagatg
     9961 caagacattc aatgaatcgc aaccacatcg gaatctcgct tatcgccgtt gttcttgtca
    10021 gcgtagtcac ttcagtcctc gctggcgtgc gaactacgca cgcctccaaa acggcacagg
    10081 ctgtggatag caaggcgatc tacacccaac gctgcgaggt gtgtcatggc gccgagggcg
    10141 acgggaacgg cccggccgcc gagcggatgt ctccccggcc acgcgacttc acaacaggcg
    10201 cgttcaagat tcgcaccacg ccctccgatt cgccgccgac cgacgccgac ctgatcgacg
    10261 tgatcagcaa cggcatgccc ggatcgacga tgccggcctg gaaggacgtg ctgtcaggtg
    10321 acgagatcgc cgccgtcgcc tcatacgtca agacgttttc ggacgccttc gagggtgaag
    10381 cgccggagtc gatccgaacc gggaatcgcg ccagcgccga tgcggcgagc atcgcacggg
    10441 gcgcggaggt gtatcgcgag gtacagtgct tcaagtgcca cggcgatgag gggcgcggcg
    10501 acgggccaag cgcgctgacg ctccaggacg agttcggcca cgagattttc ccggccgatt
    10561 tgacgcaggc gtggctgttt cggggcggcg ggacggcaga cgatatttac aagcgcatcg
    10621 tgaccgggat gaccggcagc ccgatgcctt cgttcgccga tgcgctggtg gacgccgacg
    10681 gtaaccccga tgagcagaag cgttgggacc tggtgaacta cgtggactcc ctgtcgcccg
    10741 acacgtggtc cggcccgccg gacgcactgg tggcccggcg gatcgagggt gatattccga
    10801 ccgacccgaa tgcccctgaa tggcaaagcg cgacgccgta ttacattccg ctcgtcgggc
    10861 agatcatgcg cgagccgcgc aacttcactc cctcgatcgc cggtgtgtgg gtgcgcccgc
    10921 tctacaacgc gaacgaaatg gcgctgctcg tccagtggaa cgatcgccag cagaacatgg
    10981 gtgtggaggg cgagtcgacc gacgagatcg cggtgcaatt cccggccgcg acaaccgaga
    11041 gggacgagcg tccatacttt gtcttcggcg atgcaggata cccggtaaat ctgtggacgt
    11101 gggcagcggg agcgagcacc gccatcgaaa agatcgggcg cggctcggat gctatcgcgc
    11161 cgcagccgac acagaatcta tcggcggcgg gtagttacaa tgccgggcag tattccgtaa
    11221 tctttcagcg cgcattaagt acaggcgacg tcgacgatat tgtgttaacg ccggacacgt
    11281 ttgtcccgat cgcctttgcc gcgtccgatg ggtggcgcgg cgagcagctc ggcgccggtg
    11341 cgatttcggc ctggtatccg ctctatctcg aacagcccac gccgccggca cgttacctct
    11401 gggttccgat cgctatgatc gccacggttg ctgtggaggg gctggtggta tggcttgttc
    11461 gtagggacgc gcagagaata gccggtagct gatggcggat agctgatggc tgatggctga
    11521 tggctgatgg cctatcgctc gttgacgcag gtcagacaat gtgccatcgg caatctgtga
    11581 gacgcgacga tggcccaatg gtacgtgcag ctcgtcttca aaggtgacac actgctgggc
    11641 gaaggcgtcg ccgatctggg cgaggtggtc gacatcgcat tgcccaacgg cggcgagcgc
    11701 tacacgaccg tcatcgtgtc gggctacgca ctgtacggcc aattgcgtgg ctattactcg
    11761 ctcggcattg cacacgtaac ggtcgatccg ggcagcaagc gtgtggctta tgtgtccgag
    11821 accagcgcgc tgcacaagga gccggtgggg tcgttgacgc tagcgcaacg cgcggcgatc
    11881 cgggactggc tgatgcgctt gaatccggct gcctgggaga actcgacgga ggtattcagg
    11941 cgggagttgg aaagggtgac ggagaatggc tgagcacgca aggaactcct tcagttcgcc
    12001 gagttccctc actatcatac ggaggcgacc atgcaactct acaaacttct tgtcgcgctg
    12061 attgccgtgg gtgtcttcat cgccgcctgt ggcggcggcg gcgcgccgac ggctccacca
    12121 agcggaggca ctccagaggg tggaggggag gaggtcacct cgccggtcga tccggcgacg
    12181 gccggcagcg ttgcaggcag ggtgaacttc accggcaccg cgcccgaacc cgagccgatt
    12241 ctgatggaag ccgagccgga atgccaggcg cagtatcccg acggcgcgtt cacagagacg
    12301 gtcgtcgtca acgacaatgg gacgctccaa aacgtcttcg tgtacgtcaa ggaaggtctg
    12361 ggcgatctga aggtccccac gcctcaggaa gccgtgctgt tggatcagca ggggtgccgt
    12421 tatgcaccgc acgtgctggg agtacaggtc agccaggaca ttacgattcg caacagcgac
    12481 ggcatcttgc acaacatcca tcctgtgccg acgctgaaca agagcttcaa cctgggccag
    12541 ccggtcaaga tggactcgac caggcagttc gatcaggtcg aggtgatgat cccgatccaa
    12601 tgcgacgtgc acgattggat gatcggctac gccggtgtgg tggatcatcc ctacttcgcc
    12661 gtcacgggcg ctgacggcac gttcgccttg ccggacctgc cgccgggcac gtacacgatc
    12721 gcggcctggc atgaattgta cggcgagcag acaatgcaag tgaccgtcgg cgagaaagag
    12781 acgaaggaga tcgagttcac gttcgagggc aagtaggttc cctgagtccc ttcagttcca
    12841 tgatcaggaa ctgagggaac ccccgcagac tgcgggcgag ggcaggctga aggaactgga
    12901 aggagacgac atgggcggac tgattcccca ggcctcgacg tttgccgcct caatcgattc
    12961 actgttcgtc gtcgtgctga tcgtgaccgg cgtagctttc gtgctggtcg agggcatcct
    13021 gatttacttc ctcgtccgct accgaaaccg cacggggcgg aaggccagct acgtgcacgg
    13081 aagccagcgg ctcgagctgg cgtggaccgt gggcacaggc attgccttct tcgcgctggc
    13141 gatctatcaa tataatgcct ggatccagat caaggcggcc ctgccggaca ggtctgcagc
    13201 gcagctcgtg ggtatctcgt cgaaccagtt cgaatgggaa gcgacctatc ctgccgacga
    13261 cgggcagctc ggcacggccg acgatatcaa ggctcccatt aacgtcgtgc actttccggt
    13321 gggccggccg gtgatcgtcc ggctcgagtc ggtggacgtg attcacagtt tctttgtgcc
    13381 cgagctgcga gtcaaacaag atgccgtgcc cgggcagccc atcgagttct ggttcgaagc
    13441 gacccaatcc ggccagtacg agatcgcttg cgccgaactg tgcggactgg ggcactatcg
    13501 catgcgcggc caggtgactg tcgagagtca ggctgatttc gacgcctggc tgacggaaat
    13561 caaagcgaag cggggagcgg ttggagttcc tctgagttcc tccgagttac cgtaagagag
    13621 aactcaggga actcgaggaa ctccagggaa cttatagggg gcgtcatgac cagtgacaag
    13681 gagttgcctt ttattcgccg gtacgtcttc tcgaccgatc acaaagtcat cggcaagcag
    13741 ttcctcttca ccgcgctggc cttcttcacc atcggcggtc tgctggcgct gctgatccgc
    13801 acgcagttgg cctggccgtg gcaggaggtt ccgttttttg gcaggctgct ctatggtgag
    13861 gaggctggca acattctcgc gcctgagcgg tatccggcgc tggtgacgat gcacgggacg
    13921 atcatgatct tcttcttcat cattcccgtg ctatcgggag cgttcggcaa cttcctcatc
    13981 ccgctgatga tcggcgcgcg cgacatggcc ttcccacgac tgaacatggc ctcctactgg
    14041 ctgatggtgc cggcgttcct ggtgatggtc gccagcttct tcgtggaggg tgggacggcg
    14101 gcctctggct ggacctccta cgtgccgatc agtgcgcgcc cggatatgat cgagggttcg
    14161 ggcatgggtc agacgctgtg gattatcagt ctgatcctcg tcggcacttc gtcgctgctg
    14221 ggcgccatca actacatcac gacgatcgtg acgctgcgcg cgccgggcat gagcatgttc
    14281 cgcctgccga tgactgtctg ggcgttgttt atcaccgcga tcctggtgct cctggcgacg
    14341 cccgtgctga cctccgcgct gatcctgctc ttgctcgacc gctcggctgg cacgcacttc
    14401 ttcgcggcaa acagcggcgg tcaggtcatc ctctggcagc atctgttctg gttctactcg
    14461 cacccggcag tatacatcat gatcttgccc gcgatgggca tggcttcgga catcatctcg
    14521 tgcttcgcgc gcaagccgct gttcggctac aagccgatga tcttctcgat catggcgatt
    14581 gccgtgctgg gcttcagggt ctgggggcac cacatgttcc agagttgcat gaacccgctg
    14641 ctgggcacga cgttcatgac ggcgacgatg atgatcgcgg tgccctcggc gatcaagacg
    14701 ttcaactggt tggggacact gtggcagggc aatctgcgcc tgagcgcgcc gatgctgaac
    14761 gcgctggcct ttgtctcgac gttcgtcatc ggcgggctga gcggcgtctt cgcggccgcg
    14821 acgccggtgg acattttcat ccacgataca tactggatcg tcggccacat tcactacgtg
    14881 ctgttcggcg gcagcctgtt cggcgtcttt gcggccatcc agtattggtt ccccaagatg
    14941 ttcggacgac agatgaacga tgggtggggc aagatacact tcgtgctcac cttcattgcc
    15001 ttcaactgcg tattcttccc gatgttcatc ctcggcgcga acggaatgcc acggcgcatc
    15061 gccgatgcgt atcagtatca gctcctcgcg ccgctacagt cgctcaacca gttcatgacg
    15121 gtcagcgcct acgtgcttgg cgcggcgcag ttgatcctgg ccgcgaatct cgcttggggt
    15181 ctcttccggg ggaagaaggc cgtcgacaat ccgtgggagg cgaattcgct ggaatggcag
    15241 accggctctc cgccgccgct ggagaacttc cacgccattc cgacggtcta tcgcggcccg
    15301 tacgagtaca gctcgcccga ggtgcaagag gactggctgc cgcagaatcg ttacctggac
    15361 ggcgccaagc cgcagaagaa gatgcccgcc gaagtcgggc tggctgccgg cgcagccggt
    15421 gagtaactcg acgtcggttc agaggagagt gtctatgtca cacaccaccg catccgagcc
    15481 tcacgccacc cccatctggc aagggatacc cacgaccaag tttgccatgt ggctgttcct
    15541 ggcgtcggag gtcatgttct tcgccgggct gatcggcgca tacattgtgc tgcgcagcgg
    15601 cgcgccggaa tggccggtcg tttcggaaat cctcaacgtc ccgctggtgg ccggcaacac
    15661 cttcatcctg attgtgagca gcgtgacgat ggtgcgcgcc ttcgcggcga tcgaggacgg
    15721 caacccacaa ggcatgcgcc gcatgctgat cgccaccgcc gcgctgggca cgatcttcct
    15781 gagcatacag gcggtcgagt ggagcgcgct gctgagcgaa ggcacaagtc ccagcaccag
    15841 cgtgttcggc tcggtcttct tcacgctgac gggcttccac ggcctgcacg tcctaggggg
    15901 cgtcctcacg ctggtggtcg tgatcgtcaa cgcctttcga ggtgctttca ctcaagccag
    15961 ccatggaggc gtcgaattga tggggctgta ctggcacttc gtcgacgtgg tgtggatttt
    16021 cttgtttacg atcctctact tgatatgagc tgcatgcgtt cctggagtac attcttgaca
    16081 aggaactcaa ggaactctaa ggaactcagg gaactcaggg agggcttcca tggccgaaac
    16141 gtcaattgtg caccgacgcc ccaattacat tctcgtcttc gtcttcctgg ggctgctgac
    16201 tgccgtcgag gttggcgtcg cttttaccgg gctgcctaag ccggtccaag tgccgctcct
    16261 ggtggcgctg gcgatcgcca aggcgacgct ggtagggatg tattacatgc atttacggtt
    16321 cgaggggcgt gtgctgcgat tcatcgccgt cgggccgttg ttgctggcga tcatcctgac
    16381 gctgccgccg ctcgtcgatt ccatcagtca tcactgagtt ccttcagctc cttaagttcc
    16441 ttttggctga aggaactgag ggaactgcgg ggaaccgccg cagacagcgg gcgagggcag
    16501 gctgagggaa ctatggtttc catccgaaca caccttcgtg ttccgaagac acgcgccggc
    16561 tggctgatgg gcggcatctc ggcgctggtt gtgctcagcg tggcggttct gctcgcggcg
    16621 ccgcgcggcg aaacgccggg caggttcgat gtgtctgcct tgccgcgact gaacgccgcc
    16681 ctcaacggcg ccagcgcgat cctgctcgtc gccgcttact tcttcatccg tcatcgccgg
    16741 gtgaaagccc atcgcgcgtg catgctgtcc gccttcgccg tgtcggcgct gttcctgatg
    16801 tcctacgtca tttatcatat ctttgccggc tcggttccgt ttgccgggcc gggatggatc
    16861 cggccgatct atttcgccgt cctcatctcg catatggctc tcgccgcgct cgtcttgccg
    16921 ctggccttga cgacgctgta ccgcgcctgg cggggccggt tcgttcgaca tcggcagatg
    16981 gcacgctgga cgctcccgat ctggctttac gtgtcggtga gtggggtggc ggtctacctg
    17041 atgctgtatc acttgtgaac tacgaggaac tcaaggaact gaaggaactg gggataagga
    17101 actcaaggga ctaaaggaac tgagggaact ggagggactc gcgatgacta tcgtcaccac
    17161 gcccgtcacc gactctgccc ccgcccgcgc ctggcagtcc gggccgcttt atcgcaagat
    17221 tctcatcccg accgacaact ccgagctgtg ccacgcgggg atcgattggg cgatcgagtt
    17281 cgcccgcgcc tgggatgcga cgctcgtcgg caaccacgtc tacgccgccc ggctgcacga
    17341 cgatcgcttc cggcagctcg agatcggcct gcccgcacgc ttccagaccg acgaagagat
    17401 cgcgcgccag cgcaagattc acgacaagct catcgagcag ggactcatcc tgatctccga
    17461 ctcgttcctc gacgtgctga acaagcgcgg cgaggagaac aacctgaaga tcgaacggaa
    17521 gttaatggaa ggcattcact tcgagcagtt ggcgaaggac gtcaactcag gcgactacga
    17581 tctcgtcgtg atgggggggc acggtatcgg gcaggtcaag cgatcgcaac tcgggtcggt
    17641 caccagccgc gtcgcccgac tgatcacggc cgacatgctg gtgatgaagc agaaggagcg
    17701 gtcattgagc ggtggcaaga ttctcgtgtg cgtagactcg tcggcttact gcttcgtcgc
    17761 gctgcgaaaa gccctgcggc tcgcgcagac cttcggcgcg accgtccatg tcgtcggcgc
    17821 gttcgacccg ttctatcact acgccgcgtt caacaacatt cgctacgtgc tatcgtacca
    17881 ggccggcaaa gtgttcaagt tcgaggagca ggaggagctg cacaacaac
//
LOCUS       DBNHXB010000071         5958 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4542801, whole genome shotgun sequence.
ACCESSION   DBNHXB010000071 DBNHXB010000000
VERSION     DBNHXB010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4542801"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="AC162G_02815"
     CDS             complement(<1..454)
                     /locus_tag="AC162G_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5074779.1"
                     /translation="MDRQRLLKQLDKAWAAIKESYAGLSDSQLTEPGVMGNWSVKDIL
                     AHVTTWEEEALKYLPLIIKGGRPLRYSTKYGGIDAFNAQMTEQKRGLSLSDVLRQLDE
                     THRRLIDYIQSAPEEQFTRETRFRRRLRLDTYSHYPEHAKVIREWRERS"
     gene            1021..1722
                     /locus_tag="AC162G_02820"
     CDS             1021..1722
                     /locus_tag="AC162G_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5074780.1"
                     /translation="MDDATHKSTMSDALALRRKIIGVLLQGARLRAGRTKKECAEAIG
                     VSVSTLSRFEDGRKDITLPQLELLAYMLNVPVSGFFKDKEQDRLVQEEPELPADQIIA
                     LRNRIIGVLLRQARTQADMSQKELAKALNVPPRRITQYEYGQRPVPVAELLEFAEALR
                     VPISYFLDEGVGRVGEHEQAQSQFERFAELPDDVRAFVSRYTNLPYLRVAIRISDMDA
                     DRIRRIAEGLLDITY"
     gene            1739..2272
                     /locus_tag="AC162G_02825"
     CDS             1739..2272
                     /locus_tag="AC162G_02825"
                     /inference="COORDINATES: protein motif:HMM:NF026288.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5074781.1"
                     /translation="MASGEALRDEGMRLYREERFEEAAAKFADAQAVFAANGRVKDAA
                     EQANNRGQSWRQAARWDEALAAFEEARAVFQSLGDVASEGQVIGNLGALADAQGKLDL
                     ASEYYVEAIRMLESVGERDLAQATYTALSRLKLKQGNWLGAIGAFESGLTQLEKPSLT
                     HRVVRRLLDVPRKLIGG"
     gene            complement(2328..4412)
                     /gene="fusA"
                     /locus_tag="AC162G_02830"
     CDS             complement(2328..4412)
                     /gene="fusA"
                     /locus_tag="AC162G_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR5074782.1"
                     /translation="MKDYSTDALRNVVLLGHGSAGKTMLGEAMLFSAGALSRLGRVED
                     GTTVADFDEEETRRRISLSTALLPVEWKERKLNLLDTPGFPDFAGEVKGAVRVAECAL
                     ILVDSVAGVEVGTELAWSYCDERELPRMVVVSKMDRDNADFEKALESLAGSFKANFVP
                     LFLPIGAQANLKGVVDVTRMKARMGAKGELEDISAELAGAVEDARAKVVEAAAEGDDE
                     LIMKFLDGQELTDEEVTRGLHAALRNRTLVPVLCAASTTGIGVSGVLDALVEFAPSPL
                     DAPPQVATRVASDAEETLEATDAGALAALVFKSTADPFVGKLTYFRVYRGAVTSDSRV
                     YNSTRSAEERIGQIYVMRGKEQSPVPRVGAGDIGVVAKLGVTLTGDTLCTKENAIRIA
                     PPAYPDPLITIAVEPKTQADSAKMGPTLTKLSEEDPTLRWRQEPSIKQTLLEGMGDNH
                     LDVAIRRAHSKFGVELVTHPPKVPYRESITKTAETTYRHKKQTGGAGQFAEVAMRVEP
                     QEPGKGYEFSWEVFGGAISTSFQSSIEKGIKAVMESGALAGYPIVDVKCAVFDGKEHP
                     VDSKPIAFETAGREAFKQAVLSANPVLLEPIVTLRVVVPDSYTGDVIGDLNTKRARVQ
                     GMDQEGGKAIVTASVPLAEVQRYATDLRSLTQGRGVFSMKFDRYEQTPAHIAQGIIER
                     ARKERAGEKAEE"
     gene            complement(4732..4938)
                     /locus_tag="AC162G_02835"
     CDS             complement(4732..4938)
                     /locus_tag="AC162G_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074783.1"
                     /translation="MSSFSNPHYMYLFDMKNGKKKLAYGQSPEDALEILSCRLSVEEM
                     EQIIPDQFIKISQRKMQEFVHLLA"
     gene            complement(4943..>5958)
                     /locus_tag="AC162G_02840"
     CDS             complement(4943..>5958)
                     /locus_tag="AC162G_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR5074784.1"
                     /translation="QGPGENVIVDLVSHPEKHPRVVLGVGAKSMEEWPMIDRRLWPKP
                     ASRKLQRAFNWPLEPECGWGPPAVATILTSRVCPWQCVFCNENSYIPNMGRRPVDMLI
                     DELNFLDRAYGPVGSVVIHDSMFFQNPSWLEEWLNKYPRRANKLWPYWAAARADTVRQ
                     WPDLFEALVRETNWNTVSIGFESGSDRVLRILNKECTAEDNYFTIDLLTRIGDEMERE
                     GKKPPVFWSNIMLGIPGETREDAFETMRMVKYTKRIMPSIAFYAPYPGSALGHQLIAE
                     GKSLMTKDNYHRFPDDEKVKGVDYAFYRELLAGKYDGEVNRGLPELVARRRAVYSDAL
                     VKA"
BASE COUNT         1037 a         1857 c         1859 g         1205 t
ORIGIN      
        1 ctgaccgctc ccgccattcg cgtatcacct tggcgtgctc cgggtaatgg ctgtatgtat
       61 cgagccgtag gcgacgccgg aaccgcgtct cccgtgtgaa ttgctcttcg ggcgcgcttt
      121 ggatgtagtc gatgagtcgg cggtgggttt catccagttg tctcagtacg tcggagagcg
      181 aaaggcctcg tttctgttcc gtcatctgag cattgaaggc atcgatgccg ccgtactttg
      241 tagagtaccg cagcggcctg cctcccttga tgatgagcgg cagatacttg agggcttcct
      301 cctcccatgt ggtcacgtgc gcaaggatgt ctttcaccga ccaattgccc atgacgccag
      361 gttccgtcag ctgcgagtct gacaaaccgg cgtacgactc cttgatcgcc gcccaggcct
      421 tgtcaagctg tttcagaagc cgctgtctat ccatgattcg gtttgccgac ctccgtcgat
      481 aacggcatca cccaaccacg ccaggaagca ctctatccct gtggagagaa tagcggccat
      541 agcgtaagtc acgttacggt caggctgtcg atggtctgac ggcatgcctg acgatggcgt
      601 gtcgccgttc cagtaacgat tgacggattc caaacaggtg attaggtcgg tgactgcttg
      661 actgaggtcc ggagtatacc ctccagtttt cgcatcaagc agggtgttta cgatactcga
      721 cccatgtgcc tcgctggttc catcgaaatg agcgaaggca gagtgataga agattctctg
      781 ccttgcttct tcatactcca tctgacagtc cctttatgga aatttccggc cacatcgacg
      841 caggcatagt atagtcacag acaactgggg cgtcaattgc ccctctccca aagcacgctg
      901 ttccgcttcg ggagagggga ccggggtgag ggcgcagtcg aagggacgcg ccgagagcga
      961 gccgttgaca cattgccctg tggtataatc gcgccgtgat cacctgagga gatgggatcg
     1021 gtggacgacg cgacacacaa gagcacgatg tcggacgcgc tcgcgcttcg acgcaagatc
     1081 atcggcgtgc tgctgcaggg tgctcgcctg cgcgccggcc gcaccaagaa agagtgcgct
     1141 gaagcgatcg gcgtcagcgt ctcgacgctc tcgcgcttcg aggacggccg caaggacatc
     1201 acgctgccgc agctcgagct gctggcgtac atgctgaacg tgccggtctc cggctttttc
     1261 aaggacaaag agcaagacag actggtgcag gaggagccgg aactgccggc cgatcagatc
     1321 atcgccttgc gcaaccggat catcggcgtg ctgctgcgcc aggctcgcac acaagccgac
     1381 atgtctcaga aggagctggc aaaggcgttg aacgtgccgc cgcggcggat cacgcaatac
     1441 gaatacggcc agcgcccggt tccggtcgcc gagctactcg agtttgccga ggcgcttcgg
     1501 gtgccgatat cctatttcct ggacgaaggc gtcggccgag tcggggagca cgagcaggcg
     1561 caaagccagt tcgagcgctt cgcggaattg ccggacgacg tgcgcgcgtt tgtcagccgt
     1621 tacacgaacc tgccttacct gcgggtcgcc attcgcatct cggacatgga cgcggatcgc
     1681 atccgcagga tcgcggaagg cctgctggat atcacatact aatcaccagt tactaattat
     1741 ggcgagcggc gaagcgctgc gcgacgaggg catgcggttg tatcgcgagg agcggtttga
     1801 ggaagcggcg gcgaagttcg ccgacgctca ggctgtcttc gccgccaacg gccgcgtgaa
     1861 agacgccgcc gaacaggcga acaatcgcgg ccaatcctgg cggcaagccg cgcgatggga
     1921 cgaggcgctc gcggctttcg aggaagcgcg cgccgtgttc cagtcgctcg gcgatgtggc
     1981 cagcgagggc caggtcatcg gcaacctggg cgcgctggcc gacgcccaag gcaagctgga
     2041 tctggcctcc gaatattacg tggaggcgat ccgcatgctc gaatcggtcg gggagcgcga
     2101 tctggctcag gccacctaca cggcgctaag ccgcctcaag ctcaagcagg gcaactggct
     2161 cggcgcgatc ggcgccttcg aaagcggcct gacccaattg gagaagccga gccttaccca
     2221 tcgcgtcgtg cgccgattgc tcgacgtgcc gcgtaagctg atcggcggat aagctccact
     2281 tttgagtttg ggattttgaa gtttggagtt gagcaagctc cgcgcctcta ctcttctgcc
     2341 ttctcccctg cgcgttcctt cctggctctc tcgatgatgc cttgcgcgat gtgcgccggc
     2401 gtctgctcgt agcggtcgaa cttcatgctg aacacgccgc gcccctgcgt gagcgagcgc
     2461 aggtcggtag cgtagcgctg cacctcagcc agcggcaccg acgccgtgac gatcgccttg
     2521 ccgccttcct ggtccatgcc ctgcacgcgg gcgcgcttgg tattcaagtc gccgatgacg
     2581 tcgcccgtgt agctatcggg cacgacgacc cgcaatgtga cgatcggctc gagcagcacg
     2641 gggttagcgc tgagcacggc ttgcttgaac gcttcgcggc cggccgtctc gaatgcgatg
     2701 ggcttcgagt ccacggggtg ctccttgccg tcgaagacgg cgcacttgac gtccacgata
     2761 ggatacccgg cgagcgcgcc gctttccatg accgccttga tgcccttctc gatcgacgac
     2821 tggaaggacg tggagatcgc gccgccgaag acctcccagg agaattcgta gcctttgccc
     2881 ggctcctgcg gctcgacgcg catcgccacc tcggcgaact ggcctgcgcc accggtctgc
     2941 ttcttgtgcc gataggtcgt ctcggccgtc ttggtgatcg actcgcggta gggcaccttg
     3001 ggaggatgcg tcaccagttc gacgccgaac ttgctgtggg cgcggcggat cgctacgtcg
     3061 aggtggttgt cgcccatgcc ctcgagcagc gtctgcttga tgctcggctc ctggcgccag
     3121 cgcagcgtcg ggtcttcctc ggatagcttg gtcagcgtcg gacccatctt agccgaatcg
     3181 gcctgcgtct tgggctcgac ggcgatcgtg atcagcgggt ctggataggc aggaggcgcg
     3241 atgcggatcg cgttttcctt cgtgcacagc gtgtcgccgg tcagcgtcac gccgagcttg
     3301 gctaccacgc cgatgtcgcc cgcaccgacg cgcggcacgg gactctgctc cttgccgcgc
     3361 atgacgtaga tctggccgat acgctcctcg gcgctgcgcg tgctgttgta cacgcgtgaa
     3421 tccgatgtga cggcgccccg gtagacgcgg aaataggtta atttgccgac gaaggggtcg
     3481 gccgtggact tgaagaccag ggctgccagc gcgccggcgt ctgtggcttc gagtgtctct
     3541 tcggcgtcgc ttgcgacgcg cgtcgcgacc tgcggcggcg cgtcgagcgg cgatggggcg
     3601 aactcgacca gggcgtcgag cacgcctgac accccgatgc cggtcgtcga tgccgcgcac
     3661 agcaccggca cgagcgtgcg gtttcgaagc gcggcgtgca gcccgcgcgt gacttcctcg
     3721 tcggtcagct cctgcccgtc gaggaacttc atgatcagct cgtcgtcgcc ctcggcggcg
     3781 gcctcgacga ccttggcgcg cgcgtcttcc actgcgccgg ccagttccgc cgaaatgtcc
     3841 tccagctcgc ccttcgcgcc catgcgcgct ttcatccgtg tcacgtccac gacacccttc
     3901 agattggcct gcgcgccgat cggtaagaac agggggacga aattggcctt gaagctgccg
     3961 gccagcgact ccagcgcctt ctcgaagtcc gcgttgtctc gatccatctt gctgacgaca
     4021 accatgcgcg gcagttcccg ctcgtcacag taggaccacg ccagctcggt gccgacctcg
     4081 acgccggcga cgctgtcgac cagaatcagc gcgcactcgg cgacgcgcac cgcgcctttc
     4141 acttcaccgg cgaagtccgg gaagcccggc gtgtcgagca ggttgagctt gcgctccttc
     4201 cactccacgg gcagcagcgc cgtgctcaac gagatgcgcc gccgggtttc ctcctcgtcg
     4261 aaatcggcga cagtcgtgcc gtcttcaacg cggcccaggc gtgaaagcgc gcccgcgctg
     4321 aagagcatgg cttcgcccag catcgtctta ccggccgacc cgtggccgag caggacgacg
     4381 ttccgcaggg cgtcggtcga ataatctttc atcgttcccc ctgtctggga taagagccta
     4441 tccgaagacc gtagggcggc atccccatgc cgcttgccgg cgcatgggga tgcaccagct
     4501 acggggtttt cggatagttc caagtgaatg gtatcgccat tttaagtgcg agtggcaagc
     4561 ttgtcaacgc gcctgagata caaaaaacca cccctcgcgt aacggagggg tgggcctttc
     4621 accagaagat ttttaccaca gatgacactg atgtcacgga aggtaattgc cagacatctg
     4681 tgtaatccgt gtcatccgtg gttagctttt tggttgcggt cggagacggg gctaggccaa
     4741 caggtggacg aattcctgca tcttgcgctg tgagatctta atgaactggt ccggaatgat
     4801 ctgctccatt tcttccacag acaggcggca gctcaagatc tccagcgcgt cttcgggcga
     4861 ttgcccgtag gccagtttct tcttcccgtt tttcatgtcg aacaggtaca tgtagtgtgg
     4921 attgctgaag ctgctcatgt cctcaagcct tgacgagcgc gtcgctgtac acggcgcgtc
     4981 ttcgtgcgac caattctggc agtcctctgt tgacctcgcc gtcgtatttg ccggccagca
     5041 gctcgcgata gaacgcgtag tcgacgccct tcactttctc gtcgtcggga aagcgatggt
     5101 agttgtcctt cgtcatcagg ctcttgccct ccgcgatcaa ctggtggccg agcgccgagc
     5161 cggggtaggg ggcgtagaac gcgatggagg gcatgatgcg cttggtgtat ttgaccatgc
     5221 gcatcgtctc gaaagcgtcc tcgcgtgttt cgcccggaat gccgagcatg atgttcgacc
     5281 agaagaccgg tggcttcttg ccctcgcgct ccatctcgtc gccgatgcgg gtgagcagat
     5341 cgatggtgaa gtagttgtcc tctgcggtgc attccttatt gaggatgcgc aagacgcggt
     5401 cgctccccga ctcgaagccg atcgagacgg tgttccaatt cgtctcgcgc acgagtgcct
     5461 cgaacaggtc gggccactgg cgaaccgtgt cggcgcgcgc ggcggcccag tacggccaca
     5521 gcttgttcgc ccggcgcgga tatttgttca gccattcctc cagccagctc ggattctgga
     5581 agaacatcga atcgtgtatc acgacggagc caaccggccc gtacgctctg tcgaggaagt
     5641 tcagctcgtc gatgagcata tcgacgggcc ggcgtcccat gttgggaatg tacgagttct
     5701 cgttgcagaa gacgcactgc cagggacaca cgcggctcgt caagattgtc gccacggcgg
     5761 gcgggcccca gccgcattcc ggctccagcg gccagttgaa cgcgcgctgc agcttccggc
     5821 tcgccggttt gggccagagc ctccggtcga tcatcggcca ctcttccatc gacttcgcgc
     5881 cgacgccgag gacgacgcgc gggtgcttct ccggatggct caccagatcg acgatgacgt
     5941 tttcgccggg accctggc
//
LOCUS       DBNHXB010000072         4226 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1858840, whole genome shotgun sequence.
ACCESSION   DBNHXB010000072 DBNHXB010000000
VERSION     DBNHXB010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1858840"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..1348
                     /locus_tag="AC162G_02845"
     CDS             140..1348
                     /locus_tag="AC162G_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR5074785.1"
                     /translation="MFDRPWLILLGAIVAIGFLISQPALFALPIFMYVLIGAGRWWQR
                     RVLNRVRYARRFSYTHVFPDEPVDMTIEAHNDKRLPVPWLIAQDSLPEHIHVQDATSP
                     DEPPQATLSSGIVHSFVLRGGGQSRVRLRLSSGQRGFYAIGPAVLHSGDPFSLFETMR
                     TEDRRAWLVVYPKIVPLAELGIPAKDLFGEKRAHQRIFEDPSRTMGVRDYQPGDSMRH
                     IHWKATARQGALQTRVYEHTVSTSVMICLNVATFPHAWQGVDPERLEHAIELCASIAY
                     EGAERGYAIGVAANCGAPLTGQSIHVPPNRAPDQLRHVLEALAAVTHYIVTPFDSFLL
                     RESPRVRWGATLVVVTPLTTAAILGAVSQLRDAGRRVVLISTDDEPPWVKNVQAYHVP
                     MQGETDGTRG"
     gene            1332..2879
                     /locus_tag="AC162G_02850"
     CDS             1332..2879
                     /locus_tag="AC162G_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024948.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HIR5074786.1"
                     /translation="MGHVADAASANTRSSIREKILRASEAPAGPRHAAFLAVLAAAQA
                     CLIVPWVRSIAFPLAELNPIALVFFTAFSIIASTSLVRAMDVRSAPAVLRVLLSLAGL
                     SATILIGLSMMLHPGKPIGEVLSGLGERIVHAEVIAVAEITLVGLSILAWWLGLSNAW
                     RWFTMESMQATFRAGVIVWLLFLVFNTLPTADAAAPFIAGYFAFALIALALARSQDLM
                     AQRLASRSPFGARWFVATSLMAVFLVGLSLMLWAGIVPPLVHLVWDTLTLAARLIMVI
                     GIVIIALLGGIVTGQGPSTATPGSQTTFELSEELLTELRKVDIAQAAQVLQAIIGILA
                     IVVVARLLIELARRRERLQGRSYHLLAAEDGAARISPSEIESQAGKWLQRLARQFWQG
                     PSRWLAAMTIRRIYAQTAALGKECGAPRPACATPYEYLPTLARLFPGVEPDLETITGA
                     YVRAHYGELPDTPERLGEVRQAFRRVRAEAKTVIDYNRTEFIKSQESLKQELRDRARQ
                     DQKSGFT"
     gene            3020..3391
                     /locus_tag="AC162G_02855"
     CDS             3020..3391
                     /locus_tag="AC162G_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HIR5074787.1"
                     /translation="MAKSRTDQMVERLRDLQAGTPDIEASAIVSVDGLIMASSLPAGV
                     EEDRVSAMSAAMLSLGERIAGELGRGILDEVYIHGNDGYVILMSVGQDAVLTVLARAR
                     AKLGLIFLDMKRAAEDLAKLV"
     gene            3581..>4226
                     /locus_tag="AC162G_02860"
     CDS             3581..>4226
                     /locus_tag="AC162G_02860"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR5074788.1"
                     /translation="MPKYIFCTGGVVSSVGKGVTAAAIGRVLKSRGVSVTMQKLDPYI
                     NVDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFIDENLTRASNVTTGQVYAEVISKE
                     RRGDFLGGTIQVIPHITNEIKRRIGQAARAGEHDVVIVEIGGTVGDIESLPFLEAIRQ
                     MRKDVGRDNVLYIHVTWLPRIGATGELKTKPTQHSVHELRGIGITPDVIVTRSDY"
BASE COUNT          743 a         1343 c         1355 g          785 t
ORIGIN      
        1 cgaggacgtc gtcgcgggcg tcgtcgagaa tgtgccggtg ccggtggaga agtagagatt
       61 ggtaattgga gattagagat tggagattgg aagttggaag ttggaggttt gaggtttgaa
      121 gttggacgtt ggagatcaga tgttcgatcg accctggctc atcttgcttg gcgcgatcgt
      181 cgcgatcggc ttcctgatca gccagccggc gctgttcgcg ctgcccatct tcatgtacgt
      241 tctgatcggc gcaggccgct ggtggcagcg acgcgtgctg aatcgggtgc gctacgcgcg
      301 ccgcttctcc tacacgcacg ttttccctga cgaacccgtc gacatgacca tcgaggcgca
      361 caacgacaag cgtctgcccg tgccgtggct gatcgcgcag gattcgctgc cggagcacat
      421 tcacgtccag gatgccacgt cgccggacga gccgccgcag gcgacactat ccagcggcat
      481 cgtccactcg ttcgttctgc gaggcggcgg gcagtcgcgc gtccggctgc gcctttcgtc
      541 cggacagcgc ggattctacg cgatcgggcc ggcggtgcta cacagcggcg atccgttcag
      601 cctgttcgaa acgatgcgca cagaggatcg gcgcgcctgg ctggtcgtgt atccgaagat
      661 cgtcccgctc gccgagctgg gcataccggc caaagacctg ttcggcgaga agcgcgcgca
      721 tcagcgcatc ttcgaagatc catcgcgcac gatgggcgtg cgcgactacc agcccggcga
      781 ctcgatgcgc cacatccact ggaaagccac ggcgcgccaa ggcgcgctcc agacgcgcgt
      841 gtacgagcac acggtgagca cgtcggtcat gatttgcctg aacgtcgcca ccttcccgca
      901 cgcctggcaa ggcgtcgacc cggaacggct cgagcacgcg atcgagctgt gcgcctcgat
      961 cgcgtacgaa ggcgccgagc gcggctacgc gatcggcgtg gcagcgaact gcggcgcgcc
     1021 gctgaccgga cagtcgatcc acgtaccgcc caaccgcgcg cccgatcagc tccggcatgt
     1081 gctcgaagcg ctggcggccg tcacccacta tatcgtcaca ccgttcgact ccttcctgtt
     1141 gcgcgaatcg ccgcgcgtgc gctggggggc gacgctcgtc gtggtcacgc cgctgacgac
     1201 ggcggcgatt ctcggcgctg tctctcagct ccgcgatgca ggccggcgcg tggtgctgat
     1261 ctccaccgac gacgagccgc catgggtgaa gaatgtgcag gcgtatcatg tacccatgca
     1321 aggagaaaca gatgggacac gtggctgacg cggcaagcgc aaacacgagg tcttcgatcc
     1381 gtgaaaagat cttgcgcgct tccgaggcgc cggccggccc gcgccatgct gcgtttctag
     1441 cggttctcgc ggcggctcag gcctgcttga tcgtcccatg ggttcgatcg atcgcttttc
     1501 cccttgccga gctgaatccc atcgcgctcg tcttcttcac cgccttcagc atcatcgctt
     1561 cgacgtcgct cgtccgggca atggatgtga ggtcggcgcc ggcggttctg agggtcctgt
     1621 tgtcgcttgc cgggctttcg gccacgatcc tgattggcct gtctatgatg ctgcatcccg
     1681 gcaagccgat cggcgaagtc ttgtccggcc tgggcgaacg catcgtccat gcagaggtga
     1741 ttgccgtcgc cgagatcacg ctcgtcgggc tgtcgatcct ggcctggtgg ctcggcctga
     1801 gcaacgcctg gcgctggttc acgatggagt ctatgcaggc cacgttccga gccggggtca
     1861 tcgtctggct gctgtttctg gtgttcaata cgctccctac cgccgacgcc gccgcgccct
     1921 ttatcgcggg ctatttcgcg ttcgcgctca tcgccctggc gctggcgcgc agccaggact
     1981 tgatggccca gcgcctggcg agccgctcgc cattcggcgc gcgctggttt gtggcgacga
     2041 gcctcatggc agtcttcctc gtcggtctca gcttgatgct atgggccggc atagtgccgc
     2101 cgcttgtcca tctggtctgg gatacgctca cactggccgc tcgcctgata atggtcatcg
     2161 gcatcgtgat catcgcattg ctcggcggca tcgtgaccgg gcaggggcca agcacggcta
     2221 cacctggcag ccagaccacc ttcgagcttt ccgaggagtt gctcaccgag ctgagaaaag
     2281 tcgacattgc gcaggcggct caggtgcttc aggccatcat cggcatcctg gcgattgtcg
     2341 tcgtcgcgcg cctcctgatc gagctggcgc gacggcgcga gcggctgcaa ggccggagct
     2401 atcacctgtt ggccgcggaa gacggcgcag cccgcatcag tccgtccgag atcgagtcgc
     2461 aggccggcaa atggctgcag cgcctggcgc gccagttctg gcaggggcct tcgcgatggc
     2521 tggcggccat gacgattcgg cgcatttacg ctcagacggc ggcgctcggc aaggagtgcg
     2581 gcgcgcctcg cccggcctgc gccacaccgt acgaatacct gcccacgctg gcgcgcctgt
     2641 tccccggcgt cgagccagac ttagagacca tcactggcgc gtacgtgcgc gcccactacg
     2701 gcgagctgcc ggacacgccg gagcggctcg gggaggtgcg ccaggcattc cgccgcgttc
     2761 gggccgaggc taagaccgtc atcgactaca accgcaccga attcatcaag tcgcaagaaa
     2821 gcctcaaaca agagcttcgt gacagagcca ggcaggatca gaaatcgggc ttcacgtgat
     2881 gcgtgacacg tgacgtgtag gggttgcagt gatgcctttg ctttacaagc cctatgactt
     2941 gtggtatagt ttgcgcgtca taacgtcagg caatcgctcg ctgcttcgag gcccatccca
     3001 gcacaaggag catcgccgta tggcaaagtc acgcactgat cagatggtag agcgcctgcg
     3061 ggacctgcaa gcaggtacgc cagacatcga ggcgtctgcc atcgtatcag tggacggcct
     3121 gatcatggcc tcgtcactcc cggccggggt tgaggaagac cgtgtctccg cgatgtcggc
     3181 cgccatgctg tcgctcggcg agcgcatcgc cggcgagctg ggtcgcggca tcctcgacga
     3241 ggtctacatc cacgggaacg acggctacgt gattctcatg tcggtcggcc aggacgccgt
     3301 gctgacggtg ctggctcgcg ccagggccaa actcggcctg attttcctgg acatgaagcg
     3361 ggccgccgaa gacctggcta agctggtcta aaggagagcg atgcccgcac ggtgtcgcaa
     3421 cctgaacagg caaagttgcg gtggggtggg tcgccctcaa agtgaccctc cccaccgccg
     3481 tttttgccat agagattggt gattggagat tggagattct gcacggcact caccccaatc
     3541 tctaatctct aatctcttta ataagcgagg aggtgaagcc atgcccaagt acattttctg
     3601 caccgggggg gtggtgagca gcgtcggaaa gggcgtgacg gccgcggcga tcgggcgcgt
     3661 tctgaaaagc cggggcgtca gcgtgacgat gcagaagctc gacccgtaca tcaacgtcga
     3721 tccgggcacc atgtcaccct accagcacgg tgaggtcttc gtcacggaag acggcgcgga
     3781 gaccgatctc gacctaggtc attacgagcg cttcatcgac gagaacctca ctcgggccag
     3841 caacgtcacg accggccagg tgtacgccga ggtgatctcc aaggagcggc gcggcgactt
     3901 tctgggtggg acgatccagg tcatcccgca catcacgaac gagatcaagc gccgcatcgg
     3961 gcaggcagcg cgcgccggcg agcacgacgt ggtgatcgtc gaaatcggcg gcacagtggg
     4021 cgacatcgaa agcctgcctt tcctcgaagc catccggcaa atgcgcaaag acgtcggccg
     4081 cgacaacgtc ctgtacatcc acgtcacgtg gctgcctcgc atcggcgcga cgggcgagct
     4141 taaaaccaag ccgacacagc actccgtgca cgagctgcgc ggcatcggca tcacccctga
     4201 cgtgatcgtg acccgttccg attacc
//
LOCUS       DBNHXB010000073         9840 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4375307, whole genome shotgun sequence.
ACCESSION   DBNHXB010000073 DBNHXB010000000
VERSION     DBNHXB010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9840
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4375307"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1265)
                     /locus_tag="AC162G_02865"
     CDS             complement(81..1265)
                     /locus_tag="AC162G_02865"
                     /inference="COORDINATES: protein motif:HMM:NF019428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HIR5074789.1"
                     /translation="MSRQLKTSHTVSSRTEERGQGLLEFALVLPILLVFLLGILDFAR
                     VLFIYSEISSGVREAIRFAMVQEPFDVDDCTLIQARTASTMTLTDANSLILEIWVDDG
                     EYQNTVWNCPDPGPYAAKPGERITIRARTQVRLITANVLGTILGQTFPPLPLEYFTSR
                     TIMPSEGIETGPTTTPLPTRPPKGFTPSPSSTPTDTPTNTPTPSPTATFTPGPSPTPT
                     NTPTPSNTPTNTPTPTNTPTPSITPTPSPTWTPFPSRTPTPSPTLCAGGVTCTPTPTS
                     TASRTPTSVPALRIAFEPRYPARKNNPPNVRFWVKVRVTNPVGNPVTDARVTIVEPAS
                     SFGAVLSHLGNGVYGAGGTCFSGATVDANTFVRVRAERFSYGPAEVSAWTDSNPFSNT
                     CP"
     gene            1668..2207
                     /locus_tag="AC162G_02870"
     CDS             1668..2207
                     /locus_tag="AC162G_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5074790.1"
                     /translation="MANFVHDRDEGDLVRRAQQGDADAFTELYRRHVDQIYRFVYLRV
                     SDEAAAEDLTSEVFVRALESLHAYEDRGAPFAAWLYRIANARVVDHWRRSRQVDVSLD
                     SGEIELPFEPAARDVFALKILSESLRRLTAEQQEVIILKFVEGYSTAEISQITGRSEG
                     AVKALQRRALASLARHMDS"
     gene            2207..3337
                     /locus_tag="AC162G_02875"
     CDS             2207..3337
                     /locus_tag="AC162G_02875"
                     /inference="COORDINATES: protein motif:HMM:NF039823.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HIR5074791.1"
                     /translation="MATQRPPETDFDRMLDYFMSEIQAGRLTADGCLAMYPQLTGELR
                     LSLAAAGSLAAARRAAMRPAARDAIEERLRARMAERSTLPPASHPAQAALTQRLQIAA
                     ATLLAVVVLSVGVATASASSLPGDLLYPVKRWGESVLLQLATGPGRVGALLDSAQRRL
                     EEFEALSDRGVVDVMLLEEFEADAQDAIAASEALSDAQREEAFHRVVALVTLGQEMAL
                     DTADEAGAHLSSAADAISDLRASAWRKLLSSPAPDSMRTQTPHPTRRSTRTPTSPPTS
                     SHTPWRAATPGPPAWVTPSDRPPLTPPGRGTPGAGLTWTPSGRGTPPVPQSVRPTPTP
                     PRPSPPGQGTPGSGLTSTPSERGDSDATPPGQESKPTKSKTP"
     gene            complement(3398..3772)
                     /locus_tag="AC162G_02880"
     CDS             complement(3398..3772)
                     /locus_tag="AC162G_02880"
                     /inference="COORDINATES: protein motif:HMM:NF021119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HIR5074792.1"
                     /translation="MTGKFDVDRLFSQFEEAHRQASVGAAFGTPIEAAGKTVIPIASA
                     TYGFGMGGGEGEREGRSDVGGGGGGGYMVRPLALAVIDEGGVHIQPVVNAGRIVLAGI
                     LVGAWSIFWLGRVMLRLASKKT"
     gene            complement(3874..4197)
                     /locus_tag="AC162G_02885"
     CDS             complement(3874..4197)
                     /locus_tag="AC162G_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074793.1"
                     /translation="MNPKLRHHLVRGTPIEVDGRVLVPEARVTTLTFREATLGTRRTS
                     VAGMQFSRVRPTALIERTLDGERTHRIEDVTGRALIALGIAAVAAPIMLNLLFGRLAR
                     SLPKT"
     gene            complement(4230..6833)
                     /locus_tag="AC162G_02890"
     CDS             complement(4230..6833)
                     /locus_tag="AC162G_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5074794.1"
                     /translation="MTDRPATLVLTHEHADFDAIASLLGASLITPGSIPILPRAVNRN
                     VRGFLALYGPDLPFVEPDDVPRRKVERVILVDTQTLPSVRGVTPATPVTVIDHHPPAP
                     SLPAHWTYRGEQVGATTTLVVESLAKDHIDLTQAQATLLLLGIYEDTGSLSYADTTAR
                     EAYAAGYLLESGVNLSHVREFLQHPLSETQRTLFDRLVEASQSHEISGHHVAIAAAVS
                     EENLEEISTLAHRLRDLLEPDAVFILVAFDGHIQIVARSTVEAIDVGAICQALGGGGH
                     TRAAAALVRDGNLGDVQSRLLEEIRRHARPAATVEQIMSRGGQTLDAAVSVAEASKRM
                     QRTGFEGYPVVKDGRVVGLLTRRAVDRALTHKMGGRPVSSVMEPGTVTVAPDDPVEKL
                     ERLMIGHGWGQVPVVDARGSIVGIVTRTDVLKRWAASTPPAQRAEIAARLERSLAPEL
                     LSLVRDIAATASQIGLSLFFVGGVVRDLLLGQPLYDVDFVVEGDAIALARRLALERGG
                     RVKSHARFGTAKWILDPRALHVTTPDGLHLRTLDFVTARTEFYTHPTALPQVERSSIK
                     QDLHRRDFTINAMAVWLDPDRFGELLDFYGGERDLRDGAIRVLHSLSFVEDPTRMLRA
                     VRFEQRFGFRIEPRTQHLLSGALDLLDRVSGPRLRNELELIFQEAEPEKAICRLAELG
                     ILARINPGLQCGDWLARKYRDAREALPVASLLFAYLALLTYGLEREALRELIHRLRLP
                     RDVESDLLSLAALREASGEIRDATQASAVYRLLHAHSPRALQIARLAADDPRVRANVE
                     WFTRDLRDVKPSVGGAFLQSLGIRPSPAYGRVLSALRDALLDGEVQPGPEEEAFVREL
                     VAKGNSQITDN"
     gene            complement(6992..7900)
                     /locus_tag="AC162G_02895"
     CDS             complement(6992..7900)
                     /locus_tag="AC162G_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074795.1"
                     /translation="MDAPAPQQDRVSSQRVVDRPARPIVQRTIVVRVNPRLLAALGAG
                     TIVLGAFLPWIQPEAQAALSLSHVAPVVQGWPSLLIGLIALGALLLLHADSARWVSVP
                     AAALGLAAAVIGVASAVAASNAVAAAVAQFPKASVSIVVTGPGVIVTVAGGLVCLVAG
                     LSRPPGASPEAQAVIRLGDPGFAVLASLVVLIALAGALIGGWIASSATGQPGGDASIA
                     PGDLLSTPVIDVQVTPLGVTEEPTPEVAPTLETPAVTAPPTETLPPTATEPPIPTVPP
                     SPTTESTSTPTPTPTATTGVSPLPTP"
     gene            complement(8036..9016)
                     /locus_tag="AC162G_02900"
     CDS             complement(8036..9016)
                     /locus_tag="AC162G_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR5074796.1"
                     /translation="MAHNILVWIENVKGHAAAISHEALGAARSIADGGKVTAAVFGLN
                     AGELAKEAVSLGADSAVACDDATLAEFRPEPAVALLARLARDTGAGVVMAGASTRGRE
                     VSGALAVALNAGAIADATALELQDGQIVATRPVYAGKLLSTVAPAREPVVITTRSRAF
                     PKPEADAGRSGEVASVAPVLSEDAIASKVVEFVAEGEGEVSLADARIIVSGGRGVGGP
                     EGFKPVAELARALGGALGASRAAVDAGWIPYAYQVGQTGKTVSPDLYIACGISGAIQH
                     QAGMRTSKMIVAINSDPEAPIFKLARYGVVGDLFKIVPALTAEFNKRLGK"
     gene            complement(9016..9810)
                     /locus_tag="AC162G_02905"
     CDS             complement(9016..9810)
                     /locus_tag="AC162G_02905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR5074797.1"
                     /translation="MNIVVCTKQVPDTAATLKVDEQSNVSWGDAPLVLNPWDEYAVEE
                     ALRLKEKHGGKVTVVAMGPDATREALKQAVAMGCDEAVQLWDPALDGSDAPTTAIILA
                     KAIEKIGADLVLCGKQAIDGDTGLVPPGLAHRLSWPLLAFVAKIREIDPAAKTLAVER
                     LLEEGRQVVSSRLPAVVAVVKEINEPRYPSFMGIRKASKMQPTIWSAADLGVDPAGAG
                     RGAAGVQWTRIFAPPPREGSVEFIAGDSPEDIATKLADKLMAEKVI"
BASE COUNT         1615 a         3266 c         3333 g         1626 t
ORIGIN      
        1 ttcgggagtg tgagagttag agttagagtg agagtgggga tggcgcttga acacgatagc
       61 tctcctcgac ggtatacctg tcacgggcac gtgttcgaaa acgggttact gtccgtccac
      121 gcgctgactt cggccggccc gtagctgaag cgctcggcgc gcacgcgcac aaaggtattc
      181 gcgtccacgg tggcgcccga gaagcacgtg ccgcctgccc cgtagacccc gttccccagg
      241 tggctgagga cggcgccgaa ggacgaggcc ggctcgacga tcgtaactct ggcgtcggtg
      301 actgggttac ctaccgggtt cgtgacgcgc accttgaccc agaaccgtac gttcggaggg
      361 ttgttcttgc gcgccggata gcgcggctcg aaggcgattc gcagcgcggg cacactcgtc
      421 ggcgtccggc tggcagtcga cgtcggcgta ggcgtgcacg tcacgccacc cgcgcagagc
      481 gtcgggctgg gcgtcggcgt gcgcgacggg aagggcgtcc acgtcggcga cggcgtcggt
      541 gtgatggacg gcgtcggcgt gttcgtcgga gtgggtgtgt tcgtcggcgt gttcgaaggt
      601 gtaggggtgt tcgtcggtgt cggcgaaggc ccaggagtaa acgtggcggt agggctcggc
      661 gtcggcgtgt tcgtcggcgt gtccgtcggc gtggagcttg gcgagggcgt aaatcccttg
      721 ggcggacgcg tcggcagcgg cgtggtcgtc ggcccggtct cgatgccctc gctcggcatg
      781 atcgtccgcg acgtgaagta ctcgagcggc agtggcggga aggtctggcc gaggatggtg
      841 ccgaggacgt tggccgtgat cagtctcacc tgcgtcctgg cccggatcgt gatgcgttcg
      901 ccaggcttcg cagcataagg ccccggatcc gggcagttcc ataccgtgtt ctggtattcg
      961 ccgtcgtcaa cccaaatctc caggatgagc gagttcgcgt cggtgagtgt catggtggag
     1021 gcggtacgcg cctggatcag cgtgcagtcg tccacatcga acggctcctg caccatggca
     1081 aagcggatcg cctcgcgcac gccgctcgag atttcgctgt agatgaagag cacccgcgcg
     1141 aagtcgagaa tgccgagcag gaagaccagc aagatcggca agacgagggc aaactcgagt
     1201 agcccctggc ctcgctcttc tgtgcgcgac gaaaccgtgt gtgaagtttt cagttgccgg
     1261 ctcatgccca gtctcccatt gccttgtctg ccccgacgcc gtcttgtatg agacttgagt
     1321 actggttcta cagtattgac tctagtgcca gacgagagac gcacttcctc ttcacaagag
     1381 ttagaccgta accggccaat tcccaggtac ggctcgcaca taacagtatt ataaacgatt
     1441 ccttgcgaac ttggctagcc ctaattaggt actgtatatc gcaaaatagt aagaatctgg
     1501 taagggatgg tcgcaggaat tgaacggact ggtactaaag ccgtatctaa atcacaccgt
     1561 attcggtata atggatgcac gaaaatcatc gccattcgtc gccgcgactt gtggcggcga
     1621 gcggcgggcg atgcggcgct tgatgagcgc atcgccccgg ataggtgatg gccaatttcg
     1681 tgcacgatcg cgacgagggc gatctggttc ggcgcgcgca gcagggcgac gcggatgcgt
     1741 ttaccgagct ataccggcgt cacgtcgatc aaatctatcg tttcgtatac ctgcgcgtga
     1801 gcgacgaagc ggcggccgag gatctcacga gcgaagtctt cgtccgggcg ctggagtcgc
     1861 tccacgctta cgaggatcgc ggcgcgccgt tcgcggcgtg gctgtatcgc atcgccaacg
     1921 ccagggttgt cgatcactgg cggcgctcgc ggcaggtcga cgtgtcgctg gacagcggcg
     1981 aaatcgagtt gcccttcgag ccggcggcga gagacgtgtt cgcattgaag attctgtccg
     2041 aatcgttgcg cagactgacc gccgagcagc aggaggtgat catcttgaag ttcgtcgaag
     2101 gatacagcac ggccgagatt tcgcagatca ccggacgctc cgaaggcgcg gtgaaagccc
     2161 tgcagcgccg cgcgctcgct tcactggcgc gccacatgga ttcataatgg caacgcagcg
     2221 accacccgaa accgacttcg atcgcatgct cgactacttc atgagcgaga tacaggccgg
     2281 ccggctcacg gcggacggct gcctcgcgat gtatccacaa ctgaccggtg agcttcgcct
     2341 gtcgctggcg gcggcaggct cgctcgccgc cgccaggcgg gctgccatgc gacctgcggc
     2401 gcgcgatgcg atcgaggaac ggctgcgcgc gcgcatggcc gagcggtcca cgctgccccc
     2461 cgcaagtcac ccggcgcagg ccgctctcac gcagcggtta caaatcgccg ccgcgacgct
     2521 gctggccgtc gtcgtgctga gtgtcggcgt cgccacggcg tctgccagca gcctgcccgg
     2581 cgacctgctg tacccggtca agcgctgggg tgaatcggtt ctgctgcagc tcgcgaccgg
     2641 ccccggccgc gtcggcgcgc tcctggattc cgcgcagcgg cggctggagg agttcgaggc
     2701 tctatcggat cgaggcgtgg tcgacgtgat gctgctggag gaatttgagg cagacgccca
     2761 ggacgcgatt gcagcgagcg aggcgctctc tgacgcccag cgcgaagagg cgttccatcg
     2821 cgtcgtcgct ctcgtgacac tcggacaaga gatggcgctg gacacggccg acgaggccgg
     2881 tgcccatctc tccagcgctg cagatgccat ctcggatctg cgcgcgtcgg cgtggcgaaa
     2941 actgctgtct tcgcctgcgc ccgattcgat gcggacgcag acgccgcatc caacccgcag
     3001 gtcaacgcgg acgccgacga gtcccccgac ctcaagtcac acaccgtggc gcgccgccac
     3061 gcctgggccg ccggcttggg tcacgccgag cgacagacct ccgttgaccc cgcctggacg
     3121 tggcacgccc ggcgccgggc ttacctggac gccgtcaggc cgtggtacac cgcccgtgcc
     3181 tcaaagcgtc aggccgacgc cgacgccgcc caggccgtcc ccaccagggc aggggacgcc
     3241 gggcagcggc ctgacttcca ccccatcgga acggggggac tctgatgcta caccacctgg
     3301 ccaggagtcc aagccgacta agagcaagac gccgtagaag gaaacccaaa agcgtcgggc
     3361 agacttccga agtctcgaag acttcggaag tctgctatta cgtcttcttg gacgcgagcc
     3421 gcagcatcac acgcccgagc cagaagatgg accatgcccc gacgagaata cctgccagaa
     3481 cgattctccc ggcgttcacg acgggctgga tgtgcacgcc gccttcgtcg atgacagcca
     3541 gtgccagggg ccgcaccata tagccgccgc ccccgccgcc gcccacgtcg ctgcggccct
     3601 ctcgctcgcc ctcaccgccc cccatgccga agccgtacgt ggctgacgcg atggggatca
     3661 cggtcttgcc ggcggcttcg atgggcgtgc cgaacgcggc gcccaccgag gcctgcctgt
     3721 gcgcctcctc gaactgactg aacaatcgat cgacgtcgaa ctttccggtc atggagcctc
     3781 ctgtctcagc gcccgttaca gcctatccga aaagcccgta gcatcaggcc ttgtgcctgc
     3841 agctcacacg cctgccgccc acaaggggct aggctacgtt ttcggcaggc tccgggccag
     3901 acggccgaac aatagattga gcatgatcgg cgcggcgacg gcggcgatgc ccagcgcgat
     3961 cagcgctcgc cctgtcacgt cttcgatgcg atgagtgcgc tcgccgtcga gcgtcctttc
     4021 gatcagcgcc gtcggccgca cacggctgaa ttgcatgccg gcgacgcttg tccggcgcgt
     4081 acccaatgtg gcttctcgaa acgtgagcgt cgtcacgcgc gcttccggca cgagcacgcg
     4141 cccgtcgact tcgatcggcg tgccacgcac caggtggtgg cgtagtttag ggttcacgat
     4201 gcacctcccg gtgaatcagt tatcagtaat cagttatcag taatttgtga attgcccttc
     4261 gccacaagct cgcgcacgaa cgcttcttct tcaggacccg gctgcacttc tccgtcgagc
     4321 agcgcatcgc gcagcgcgct cagcacgcgg ccataggccg ggctgggccg gatgccgagc
     4381 gactgcaaga acgctcctcc gaccgatggc ttaacatctc gaaggtctcg cgtgaaccat
     4441 tcgacgttgg cgcgcacgcg cggatcgtcc gccgccaggc gcgcgatctg gagggcgcgc
     4501 ggcgagtgcg cgtgcagcag gcggtacacg gcgctggcct gcgtcgcatc gcggatctcg
     4561 ccgctcgcct cgcgcagcgc cgccaggctg agcaggtcgc tctctacgtc gcgcggcagc
     4621 cgcaggcggt ggatcaactc gcgcagcgcc tcccgttcca gtccatacgt gagcagtgcc
     4681 aggtacgcga acaagagcga cgccacgggc agcgcctcgc gcgcgtcgcg atattttcgc
     4741 gccagccagt caccgcattg caggccgggg ttgatccgcg ccaggatgcc cagctcggcc
     4801 aggcggcaga tggccttctc cggctcggcc tcctggaaga tcagctccaa ttcgttgcgg
     4861 aggcgcgggc cgctcacgcg gtcgagcaga tccagcgcgc cgctcagcag gtgctgcgtg
     4921 cgcggctcga tgcgaaagcc gaaacgctgc tcgaagcgca cggcgcgcag catgcgtgtg
     4981 gggtcttcga cgaacgacag cgagtgcagc acgcggatcg cgccgtcgcg caggtcgcgc
     5041 tcgccgccgt agaagtcgag cagctcgccg aagcgatccg ggtcgagcca cacggccatg
     5101 gcgttgatcg tgaaatcgcg gcggtgcagg tcctgcttga tcgacgatcg ctcgacctgc
     5161 ggcagcgccg tggggtgcgt gtaaaactcg gtgcgcgccg tgacgaagtc gagcgtgcgc
     5221 aggtgcaggc catccggcgt cgtcacgtgc agcgcgcgcg gatcgagtat ccatttagcc
     5281 gtgccgaagc gcgcgtggct tttcacgcgc ccgccgcgct ccagcgccag gcgccgcgcc
     5341 agcgcgatcg cgtctccctc cacgacgaag tcgacgtcgt acagcggctg cccgagcaag
     5401 agatcgcgca cgacgccgcc gacgaagaac agcgacaagc ctatctggct cgccgtggcc
     5461 gcgatgtcgc gcacgagcga cagaagctcc ggcgcgagcg agcgctcgag gcgtgcggcg
     5521 atctcggcgc gctgcgccgg cggcgtgctg gccgcccacc gcttcagcac atcggtgcgc
     5581 gtgacgatgc cgacgatgct ccccctcgcg tcgacgaccg gcacctgccc ccagccgtgc
     5641 ccgatcatca ggcgctcgag cttctcgacc gggtcgtcgg gcgcgacggt caccgttccg
     5701 ggttccatca ccgacgacac ggggcggccg cccatcttgt gcgtgagcgc ccgatcgacc
     5761 gcgcgccgcg tcagcagccc gacgacgcgg ccgtccttga cgacggggta gccctcgaat
     5821 ccggtgcgct gcatgcgctt tgacgcttcg gcgacgctga cggcggcgtc gagcgtctgc
     5881 ccgccgcgcg acatgatctg ctcgacggtg gcggccggcc gcgcatgcct gcgaatttct
     5941 tccaagaggc gtgactgcac gtcgcccaga ttcccgtcgc gcacgagcgc cgccgccgcg
     6001 cgcgtgtgcc cgccgccacc aagcgcctgg cagatcgcgc cgacgtcgat ggcctcgacc
     6061 gtgctgcgcg ccacgatctg gatgtgcccg tcgaacgcga cgagaatgaa gacggcgtcc
     6121 ggctcgagca ggtcccgcag gcggtgcgcc agcgtcgaga tttcctcgag gttctcttcg
     6181 ctgaccgccg ccgcgatcgc gacgtggtgg ccgctgatct cgtgcgactg tgacgcctcg
     6241 accaggcggt caaacagcgt ccgctgcgtc tcggacagtg gatgctgcag gaactcgcgc
     6301 acgtgcgaca ggttcacgcc gctttccagc aggtagccgg cggcgtacgc ttcgcgcgcg
     6361 gtcgtgtccg cgtacgacag ggagccggtg tcttcgtaga tgccgagcaa gagcagcgtc
     6421 gcctgcgcct gcgtcaggtc gatgtgatct ttcgccaggc tctcgacgac gagcgtcgtc
     6481 gtcgcgccca cctgctcgcc gcgatacgtc caatgagccg gcagcgacgg cgccggtggg
     6541 tggtgatcga tgacggtcac cggcgtcgca ggcgtcacgc cgcgcaccga gggcagcgtc
     6601 tgcgtgtcga ccaggatgac ccgctcgact ttgcgacgcg gcacgtcgtc cggctcgacg
     6661 aatgggaggt ccggtccgta cagcgcgagg aatccgcgca cgttgcggtt gaccgcgcgc
     6721 ggcagaatag ggattgagcc gggcgtgatg agcgacgcgc cgagcagcga ggcgatggcg
     6781 tcgaagtctg cgtgttcgtg cgtcaagacg agcgtggcag gccgatcggt catcgactgc
     6841 gattatatgc caactccgcc attcacgcat agggcaagta tcccgctatc gagtgtgcac
     6901 gcacaacatg ccaggccgct caccgcccgc gcccgcgcgg gcggtcaacc gaatactcgc
     6961 ctatccgtgt aatccgtggg aaatccgtgt cttagggcgt gggcaggggc gatacgccgg
     7021 tcgtcgcagt cggagttggc gtcggcgtcg acgtggattc cgtcgtcggc gacggcggca
     7081 cagtcgggat gggcggctcg gtcgcagtag gcggtaaggt ctcagtgggc ggagccgtca
     7141 ccgcgggcgt ctcgagcgtc ggcgcgacct caggcgtcgg ctcctccgtc acgccgagcg
     7201 gcgtcacctg cacgtcgatg acaggcgtcg acagcaaatc gcccggcgcg atcgacgcat
     7261 cgccccctgg ctggccggtc gcgctggatg cgatccaccc gccgatcaaa gcgccggcca
     7321 atgcaatcag caccacgagc gaggccagca ccgcgaagcc agggtcgccg aggcggatga
     7381 ctgcctgggc ttccggcgac gcgccgggcg gacgggagag tccggcgaca aggcacacca
     7441 aaccgccggc gactgtgacg atcacacccg gtcccgtcac gacgatcgag acgctcgctt
     7501 tggggaattg agcgacggcc gcggcgactg cgttcgaagc cgcgacggcg ctggcgaccc
     7561 cgatgacggc ggcggccagc cccagggccg ccgccggaac cgacacccag cgcgcggagt
     7621 cggcatgtag cagcaagagc gcgccgagtg caatcaagcc gatcaggagc gacggccagc
     7681 cttgcactac gggggccacg tgcgacaggc tgagcgcggc ctgcgcttcc ggctggatcc
     7741 agggaaggaa cgcgccgagc acgatcgtgc ctgcgcccag cgcggcgagc aggcgcgggt
     7801 tgacgcgcac gacgatcgtg cgctgcacga tggggcgggc agggcggtcg acgacgcgct
     7861 gactgctgac gcgatcttgc tgcggggccg gggcatccat tgcaaggatt atgcctcagt
     7921 caggagagac gcgcaaatgg cagcggatga gaaccgctag aggaatgcga cagattaaca
     7981 acggacaagc ggatggtgaa cctcgcatcc gtttatccgt tgtatattcg ttgccctact
     8041 tgccaagacg tttgttgaac tcggcggtga gcgccgggac gatcttgaac aggtcgccga
     8101 cgacgccgta gcgcgccagc ttgaagatcg gcgcttcggg gtcagaattg atggcgacga
     8161 tcatcttcga cgtgcgcatg cccgcctgat gctgaatcgc gccgctgatg ccgcaggcga
     8221 tgtacaggtc gggtgagacg gtcttgccgg tctggcccac ctggtacgcg tacgggatcc
     8281 accccgcgtc gacggcggcg cggctggcgc cgagcgcccc gcccagcgcg cgggccagct
     8341 cggcgaccgg cttgaacccc tccggaccac caacgccacg accgccggac acgatgatgc
     8401 gcgcgtcggc caggctgact tcgccttcgc cctcggcgac gaattcgacg accttgctcg
     8461 cgatcgcgtc ttcgctcaag accggcgcaa cgctcgccac ttcgccagac cggccggcgt
     8521 cggcttcggg cttggggaag gcgcgcgacc gcgtcgtgat gaccaccggc tcccgggcgg
     8581 gcgcgacagt cgacagtagc ttgccggcgt acaccggccg cgtcgcgacg atctggccgt
     8641 cttgcagctc gagcgccgtc gcgtcggcga tcgcgccggc attcagtgcg acggcgagcg
     8701 cgccgctcac ctcgcgcccg cgcgtcgacg cgccggccat gaccacgcct gcgccggtgt
     8761 ccctggcgag cctggccagg agggcgacgg ccggctcagg ccggaattcg gcgagcgtcg
     8821 cgtcgtcgca cgcgacggcg ctgtccgcac cgagcgagac ggcttccttc gccagctcgc
     8881 cagcattgag gccgaagacg gccgccgtca ccttgccgcc gtcggcgatg gagcgggccg
     8941 cgcccagcgc ctcgtgcgag atggcagcgg cgtgaccctt gacgttttcg atccagacga
     9001 ggatgttgtg cgccatcaga tcaccttctc cgccatcaat ttgtcggcga gcttcgtcgc
     9061 gatgtcttcc ggcgagtcgc cggcgatgaa ctcgacgctc ccttcacgcg gcggcggcgc
     9121 gaagatgcgt gtccactgca cgccggccgc gccgcgtccc gcgccggccg gatcgacgcc
     9181 caggtcggcg gccgaccaga tggtcggctg catcttcgac gctttgcgga tgcccatgaa
     9241 cgacgggtag cgcggctcgt tgatctcctt gacgacggcg acaacggcgg gcagccggct
     9301 cgagacgacc tgccggcctt cttcgagcag ccgctcgacg gcgagcgtct tggcggccgg
     9361 gtcgatctcg cggatcttgg cgacaaaggc gagcagcggc cagctgaggc ggtgggcgag
     9421 gccgggtggc accaggcccg tgtcgccgtc gatggcctgc ttgccgcaga ggacgaggtc
     9481 tgcgccgatc ttctcgatcg ctttggcgag gatgatcgcg gtcgtcggcg cgtccgatcc
     9541 gtcgagggcc ggatcccaca actggacggc ctcgtcgcag cccatggcca cggcctgctt
     9601 gagcgcctcg cgtgtggcgt caggtcccat cgccacgacg gtcaccttgc cgccgtgctt
     9661 ctccttcaag cgcagcgcct cttcgactgc atattcgtcc cacggattga gcaccagcgg
     9721 cgcgtcgccc caggagacgt tcgactgttc gtcgaccttg agcgtcgcgg cggtgtcggg
     9781 gacttgcttg gtgcagacga cgatgttcaa gtggctacct ccttgtcagt ggaaattggt
//
LOCUS       DBNHXB010000074         6923 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3872010, whole genome shotgun sequence.
ACCESSION   DBNHXB010000074 DBNHXB010000000
VERSION     DBNHXB010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3872010"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            440..748
                     /locus_tag="AC162G_02910"
     CDS             440..748
                     /locus_tag="AC162G_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074798.1"
                     /translation="MADKTDDALFVAGKAKWLLDNAEKLTPDQRALLLAVADLEEGRG
                     RKLSAEERAAIDRLAETQEGYNPKDIEQAVQYMLEAKTKRKVVDWPSGLWAKFRKRKS
                     "
     gene            complement(792..1565)
                     /locus_tag="AC162G_02915"
     CDS             complement(792..1565)
                     /locus_tag="AC162G_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR5074799.1"
                     /translation="MDRVSISCERLSDLEGHQALAESCKLGLELQEFSNPTLLDNDWH
                     TRLREYQSSLNGFVQPITMHGAFIDLISGSPDRRVAALARDRYRHNLEIAHALSATVI
                     DFHANYLPLVDHPSYLPDWENRQVDFWGPLAEEAARIGVTLVLENMWEPDPRIIGRVL
                     KRINSPNLKACLDVGHASLYSRLPIGEWIKDLGEDLIYTHLHNNHGTTDEHLAFGDGV
                     IDFPELLATLRALPKPPMFILELPNLESIQASLPYLELE"
     gene            complement(1829..2440)
                     /locus_tag="AC162G_02920"
     CDS             complement(1829..2440)
                     /locus_tag="AC162G_02920"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5074800.1"
                     /translation="MIRALITDFGGVLVRTASAAPRRALEARLGLPPHTIEDRVFAGD
                     ASLRGQRGEMSFAAVWEQIGRDLELNGRMSVQEFRDLFFSADFLDVELMAFIHSLRPA
                     FKTGLLSNAWDNARQVFTQIFPIADAFDAIVISAEEKVAKPDPRIYRIALERLGVQAG
                     ESVFLDDMPENVRGAEAVGMRAVQFRSTVQAMREIGQLLGLGG"
     gene            complement(2571..3839)
                     /locus_tag="AC162G_02925"
     CDS             complement(2571..3839)
                     /locus_tag="AC162G_02925"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR5074801.1"
                     /translation="MPAASPTPLGGGQAAAATVDQTAPAAASTVDPETTARHLRIFEQ
                     LWNTVDTQYVYADFNGVDWLAVYGEFKSRVESGLSDEGFWQAMSEMIARLDDKHSTFL
                     TPDEARKEDAQLKGDLSYVGIGVYAGTQPEKQQAVIFSVLPGSPADEAGLRAHDAILT
                     IDGQPAAGGDGGDNLDLLHGRPGTQTVLTVRSPGEAPREVRLTRRRIGGTLLATGRIL
                     PVEGTSLRIGYLAIPTFWDASIAIGARQALAELMAAGPLDGLIIDMRINGGGVSTNLL
                     ALLGFFTSGPHGEFAGRDQARPLDVTAEPVGNSQDVPLAILTGPHTQSYAEVFSGVLR
                     EAGRARLVGMTSPGNVETIYGYDFEDGSRAWIAREGFTSASGADWEGIGLVPDSIVDA
                     DWDEFTEENDPYIAAALALFAASAGAAAPR"
     gene            complement(3983..4945)
                     /locus_tag="AC162G_02930"
     CDS             complement(3983..4945)
                     /locus_tag="AC162G_02930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR5074802.1"
                     /translation="MSLPDFASRLATGDLFILDGATGTELTRRGVDTTLPLWSAGALI
                     DPQGRDVLRRIHGDYIRAGAHIVTANTFRTHRRSLAKGGHGIRARELTHLAVQLVRQA
                     VEQVPTCHRVFVAGSIAPLEDCYSPQLVPPDGELRTEHAEMARHLAEAGVDVMLVETM
                     NTIREARIAVEAARSAGLPVMVSFVCAGATPLGGARAAGEPVRPGDEPRLLSGERLSD
                     AVRAVESLEPAAIMVNCAPVALIEGLLRALRAATLRPIGAYGNVGHVDERVGWTLTHA
                     ITPGQYAAAARGWRALGASVIGGCCGTTPEHIAALAQALHPQPA"
     gene            complement(5146..6393)
                     /locus_tag="AC162G_02935"
     CDS             complement(5146..6393)
                     /locus_tag="AC162G_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074803.1"
                     /translation="MRKLANLRRLRITVLGLLAVMLIAACQGTAAREVPSTGVAPTAS
                     PTGPPTPVASPTPQPIPTPSPTPTVEPTPPPVVEQARTDLAGRLGRTVSDIEIRAVES
                     RQWNDGCFEIPLPASGCFPISVPGYRVTLAVGDTEFEYHTDIDHRVAIAPTSSLMLYR
                     LKYPLVWLEVRREIETEGFCDMWRVFEDGWGGWEAPCRRTGLTFYPSPERFHALREVV
                     LISASFTEQIEDPYLMTGYLYGLGAEAPSDYARAVVRDFMRWLANPAGEEPPGTCTYT
                     HPLYAYALDYPCNWEVEEETASFHSPGRGGPYAPPAAKISVYTTPPDPKPKAETPVPL
                     TLADLEQWVLGEINESGVNIVERQEIVLNGQPAVRIATEDELGRYLTYLLVVNGRDYV
                     LSGLGDLTAAEAVMRTFRVLSGP"
     gene            complement(6425..>6923)
                     /locus_tag="AC162G_02940"
     CDS             complement(6425..>6923)
                     /locus_tag="AC162G_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074804.1"
                     /translation="AAGTGDDTSWLTVEPPADMTWQSFVGKIEAKDIALGYELLYPAD
                     WFVYSDGGGGSIQTFAQFETPDQAPPGFLDGNMKIDVGFQICAPLSGDNCQLIGTPVV
                     VAGLPGVAQAHKSELTGAEFRAVALQKGYVTLTIAAHIHGEAERVKAYEQILGYMLST
                     LRVDE"
BASE COUNT         1278 a         2156 c         2215 g         1274 t
ORIGIN      
        1 tcctcgacgc tcaggccgtg atcgagttgg gtttccaggg cgcgggcgac agtctcgatc
       61 gtatggctat accagggggt cgcggttccg gggatttcca tgcgggacgc agctccaaaa
      121 tgggaagttg tgctacggta aaacactgcc ttgaagagat tatagcatag accgacaggc
      181 tgcccgatct ttggcggacg attatctccc cggtgcgtga catccacctg cgcttgggat
      241 tgcgcttggc ggacttgcgc aatcggatat ataatcaggc cgagccgagt gaggtcattg
      301 tatcgagcgt acgccgaatc cttgggcgtc ggagtcgagc ctgtgcgaag ccccctttag
      361 ggtcaggcgg atccggctaa agcctcgaat ccactcgaga ttttcaagtg ccggtagtat
      421 gcgttcgaca gaggacacca tggcagacaa aacggacgac gccctgtttg tggccggcaa
      481 agccaaatgg ctgctcgaca acgccgagaa actcacgccc gaccagcgcg ccttgctgct
      541 ggccgtcgcc gatctggaag agggccgcgg ccgcaagctg tctgccgagg agcgcgcggc
      601 tatcgatcgg ctcgccgaga cgcaagaagg ctacaatccc aaggatatcg agcaggccgt
      661 gcagtatatg ctcgaagcca agacaaagcg caaggttgta gactggccaa gcgggttgtg
      721 ggcgaagttt cgcaagagga agagttagag tttggagtta gagttggaga ctggcggcag
      781 ccggtctcct tttattccaa ctcaagatac ggcaggctgg cttgaatcga ttcgaggttg
      841 ggtagctcca ggatgaacat cggcggcttg ggaagcgcgc gcagcgtggc cagcagctct
      901 gggaagtcga tcacgccgtc gccaaatgcc agatgctcgt cggtcgtgcc gtgattatta
      961 tgaagatgag tatagattaa atcttccccc aggtctttga tccactcgcc gatcggcagc
     1021 cgcgagtaca ggctcgcgtg tcccacgtcg aggcaggcct tcaggttggg cgagttgatg
     1081 cgtttcagga cgcgcccgat aatgcgcggg tcaggctccc acatattttc caggacgagc
     1141 gtgacgccga tccgggccgc ctcttcggcg agcggtcccc agaagtcgac ctgccggttt
     1201 tcccagtcgg gaaggtagct ggggtggtcg accagaggca aataattggc gtgaaagtcg
     1261 atcaccgtcg cgctcaacgc gtgcgcgatc tcgaggttgt ggcggtagcg atcgcgcgcc
     1321 agcgcggcga cgcgccggtc cgggctgccg ctgatcaagt cgatgaacgc gccgtgcatc
     1381 gtaatcggct ggacgaagcc gttcaggctc gactgatact cgcgcagccg ggtgtgccag
     1441 tcgttgtcga gcaaggtcgg gttggaaaac tcctgcagct ccagcccaag cttgcacgat
     1501 tcggccagcg cctgatgacc ctccaggtct gacaatcttt cacacgagat ggagacgcga
     1561 tccatgcaat ttccctgacc ggctttgcat ttattgtaca gcatatccat gctttgtgcc
     1621 aaaccggtta ccaacgatcg cataacgatt ccggcgcgag tgttcgctcc tcgggcaggc
     1681 gcctgtgcag ccagacacat tcgccaggaa gagcatatgc ctgcctgacg ctgtatcaaa
     1741 tgggaagttt ggcagggccg agcgcctcgc ctcacgatat gaaacgactt tgcacggttc
     1801 gccgggttac agttcgatta ctgggggttc aaccgcccag cccgaggagt tgcccgatct
     1861 ctctcatcgc ctgcacggtc gaccgaaact gaacggcgcg catgcccacc gcctcagcgc
     1921 cgcgcacgtt ctccggcatg tcgtccaaga aaacggactc gccagcctgc acgcccaatc
     1981 gctccagcgc gatccggtag atgcgcggat cgggcttggc caccttctcc tcggccgaaa
     2041 tgacgatcgc gtcgaaggca tccgcgatgg ggaagatctg agtgaacacc tggcgcgcat
     2101 tgtcccaggc gttgctgagc agaccggtct tgaaagcagg gcgcaggctg tggatgaatg
     2161 ccataagctc cacgtcgagg aagtccgcgc tgaaaaagag gtcgcggaac tcctggacgc
     2221 tcattcggcc attcagctcc aggtcgcggc cgatctgttc ccagacggcg gcgaagctca
     2281 tctcgcctcg ctggccgcgc agcgaggcat cacccgcgaa cacgcggtct tcaatcgtgt
     2341 gaggcggcag tccgaggcgc gcttcgagcg cgcgtcgagg ggcggcgctg gcggtgcgca
     2401 ccagcacgcc gccgaagtcg gtaataagag cacggatcaa cagtccactc cttcgaggcc
     2461 cggcggctct ttcgccgcgt ccccgcctga ttacgggcga ccgcgactcg ctcgcttcgc
     2521 tcggagcgct tcgcaggggt cgcccctacg tttacgataa ggccctgatc tcagcgcggc
     2581 gccgccgcgc cggccgaagc cgcaaagagc gccaaggccg cggcgatgta cggatcgttc
     2641 tcttcggtga actcatccca gtcggcgtcg acgatgctgt ctggaaccag gccgatccct
     2701 tcccagtcag ctccgctcgc cgaggtgaac ccctcacggg cgatccacgc ccgcgatcca
     2761 tcttcgaagt cgtaaccgta gatcgtctcg acgttgcccg gcgaggtcat ccccaccagc
     2821 cgcgcgcgcc cggcttcgcg caacaccccg ctgaaaacct cggcgtagct ttgtgtgtgc
     2881 ggccctgtca agatggcgag cggcacatcc tgcgagttgc cgaccggctc ggccgtcaca
     2941 tcgagtgggc gtgcctggtc acggccggcg aactcgccgt gagggccgct ggtgaagaac
     3001 cccaacagcg ccagcaggtt agtgctcacg ccgccgccgt tgatgcgcat gtcgatgatc
     3061 aagccgtcca gcgggccggc agccattaat tccgccagcg cctgacgcgc gcctatcgca
     3121 atcgaagcat cccaaaacgt gggaatcgcc aggtatccga ttcgcaggct cgttccctcg
     3181 accggcagga tgcgccctgt cgccagcagt gtgcctccga tccggcggcg cgtcaggcgc
     3241 acctcgcgtg gcgcttctcc gggcgagcgg acggtcagca cggtctgcgt gccgggtcga
     3301 ccgtgcagca ggtcaaggtt gtcgccgccg tcgccgccgg cagccggctg gccgtcgatc
     3361 gtcagaatgg cgtcgtgcgc gcgcaagcca gcttcgtcgg ccgggctgcc aggcaagaca
     3421 ctgaagatca cggcctgctg cttctcaggt tgcgtgcctg cgtagacgcc gatacctacg
     3481 tatgacaggt cgcctttgag ttgggcgtcc tctttccgcg cttcgtccgg cgtcaggaat
     3541 gtagagtgct tgtcgtcaag ccgggcgatc atctcgctca tggcctgcca gaatccctcg
     3601 tcactcaggc cgctttcgac tctggacttg aactcgccgt atacggccag ccagtccacg
     3661 ccgttgaagt cggcatacac gtactgagta tccactgtat tccacaattg ctcgaagatg
     3721 cgcaagtgac gggcagtggt ttccggatcg acggtggacg cagccgccgg cgccgtctga
     3781 tcgacggtcg ccgccgctgc ttgccccccg cccagcgggg ttggcgacgc ggcaggcatt
     3841 tcgataggcg ccgccgcggg tatcgatacg gtcgccggcc ggattgcggt tgccgtcgcc
     3901 gggacaggca acggagtgcg tgggacgcac gccgaaacca gtatgacgaa gacggaaaac
     3961 agaaggaaga gtttcgttga tctcatgcgg gctgtgggtg aagcgcctgg gccaacgcag
     4021 cgatgtgctc gggtgtagtg ccgcagcagc cgccgatgac cgacgcgccg agagcgcgcc
     4081 agccacgtgc agcagccgcg tactgtcccg gcgtgatggc gtgcgtgaga gtccacccga
     4141 cgcgctcgtc gacgtgtccg acgttgccgt acgcgccgat cggcctgagc gtggcggcgc
     4201 gcagggcacg cagcaggccc tcgatcaggg cgaccggcgc gcagttcacc ataatcgcgg
     4261 ccggttcgag cgactcgacc gcccgtaccg cgtcgctcaa tcgctcgccg ctgagcaggc
     4321 gcggctcgtc gcccggccgg accggctctc ccgctgctcg cgccccgccc agcggggtcg
     4381 cgcccgcgca tacgaagctg accatgacgg gcaggccggc gcttcgcgcg gcctctacgg
     4441 cgatgcgcgc ctctcgaatc gtgttcatgg tttcgaccag catcacgtcg acgcctgcct
     4501 cggccagatg ccgggccatc tcggcgtgct ctgtcctgag ttcaccatca ggcgggacga
     4561 gctgcggcga gtagcagtct tcgagcggcg cgatagagcc ggcgacgaag acacgatggc
     4621 aggtgggaac ctgctcgacg gcctggcgca caagctgaac ggcaaggtga gtcagctcgc
     4681 gcgcgcgaat gccgtgaccg cccttagcca ggctgcgccg gtgcgtgcgg aacgtgttcg
     4741 ccgtgacgat atgcgcgccg gcgcgaatgt aatcgccgtg aatgcggcgc aggacgtcgc
     4801 gcccctgcgg gtcgatcaac gcgccggccg accataacgg gagcgtggtg tcgacgcccc
     4861 ggcgcgtcag ctccgtgccc gtcgcgccgt cgaggatgaa gagatcgccc gtggcgagtc
     4921 gcgatgcgaa gtcaggcagg ctcatgctca gatacggtta gtggtcgagg atgcatggga
     4981 cgcttcgcgt gagggtgtag atacggtctg cgacaaccag taccgattat accgcagccc
     5041 tcgtattgct gtggcatgtc acggtgttcc ggctggataa agaataagcc cccaactcgg
     5101 ctgggggctt atgatagtgg gggctcaccg gaggacaggg gagcgttaag gtccgcttaa
     5161 cacccgaaac gtgcgcatca cagcttcggc agcggtcagg tcgcccagtc cgctgaggac
     5221 gtaatcgcga ccgttgacga ccaacaagta ggtgagatac cgccccagtt catcttcggt
     5281 ggcgatccgc acagccggct ggccattcag cacgatctcc tgacgttcga cgatatttac
     5341 cccagactca ttgatctcac ccagcaccca ttgttcgagg tcggccagcg tcagaggcac
     5401 cggtgtttcc gccttcggtt tgggatctgg cggtgtagtg taaacgctaa ttttggcggc
     5461 cgggggggca tacgggccac cccgcccagg cgagtggaaa gaggccgtct cctcttcgac
     5521 ttcccagttg cacggataat ccagggcata ggcgtagagc ggatgagtgt aggtacacgt
     5581 acctggcggc tcttccccgg ccgggttggc cagccagcgc atgaagtcgc gcaccacggc
     5641 gcgggcgtag tctgaaggcg cttcagcacc caggccatac aggtagccgg tcataaggta
     5701 gggatcctcg atctgttcgg tgaacgaggc cgagatgagc accacttcgc gcaacgcgtg
     5761 gaaacgctcc ggagaggggt agaaagtaag cccggtacgg cgacagggag cttcccagcc
     5821 gccccatcca tcttcgaaca cgcgccacat gtcacagaag ccctctgtct cgatctctcg
     5881 acgaacctcc aaccagacga gagggtactt caacctgtag agcatcagcg atgaagtggg
     5941 tgcgatcgcg acccggtgat cgatgtcggt gtgatattcg aattctgtgt ctcccactgc
     6001 cagggtgacc cggtatcccg gcacagagat cgggaagcag ccagaagctg gcagtggaat
     6061 ctcgaagcat ccgtcgttcc attgtcggga ttccacagcc cggatttcta tgtccgagac
     6121 cgtccgtccc aaacggccgg cgaggtcggt gcgtgcctgc tcgacgacgg gcggcggcgt
     6181 cggctcgacc gttggtgtgg gcgagggggt cggtattggc tgaggggtgg gcgacgcgac
     6241 cggtgtcggc gggccagtcg gcgaggctgt cggcgcgacg ccggtcgatg gcacctcacg
     6301 cgctgctgtc ccctggcagg ccgcgatgag catcacggcc agcaagccca gcactgtgat
     6361 acgcaacctg cgtaggttgg cgagtttccg catggcagct tcccgacacc gcaacacatg
     6421 gctttcattc gtctaccctg agtgtcgaga gcatatagcc caggatttgc tcgtaagcct
     6481 ttacccgctc tgcctcaccg tggatgtggg cagctattgt aagggtgacg tatcctttct
     6541 gcaaggcgac tgcacgaaat tccgcgccag tgagttcact cttgtgagcc tgcgccactc
     6601 ccggcaaccc cgccacgacg acgggtgtac cgatcaattg gcagttgtcg ccggacagag
     6661 gtgcacaaat ctggaagccg acatcgatct tcatgttgcc atcgaggaac cccggtggtg
     6721 cctggtcggg cgtttcgaat tgtgcgaagg tctgtatact cccaccgccg ccatccgaat
     6781 agacaaacca gtcagccggg tagagcaact cgtagccgag cgcgatgtcc ttcgcttcga
     6841 tcttcccgac gaaactctgc caggtcatgt ccgccggtgg ctcgacggtc agccaggaag
     6901 tgtcgtcgcc cgtccccgcc gct
//
LOCUS       DBNHXB010000075         8841 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2362851, whole genome shotgun sequence.
ACCESSION   DBNHXB010000075 DBNHXB010000000
VERSION     DBNHXB010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2362851"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="AC162G_02945"
     CDS             complement(<1..886)
                     /locus_tag="AC162G_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5074805.1"
                     /translation="MRADRLLSILLLLQVHQRLTARELARRLEVSERTIHRDMEALSA
                     AGVPVVAERGIGGGWRLLDGYRTNLTGLNEVEIQALFLGRPPRLLADLGLDRASEAAF
                     IKLLAALPSMSRRGAEYARQRIHVDPGGWRQPDEAIHFLPALQEAVWQERKVRITYRR
                     GDGSTVERVVDPLGLVAKGSIWYLVAAVEGDFRTYRVSRVQDAQLTDEPCVRPPDFDL
                     AAYWEQSSAEFKANLPRYPAALRVAPHILPRMRAEGRYARIEHEYPPDADGWIRVDMM
                     FEEEHGAVEYVLSYGPRIE"
     gene            1004..1840
                     /locus_tag="AC162G_02950"
     CDS             1004..1840
                     /locus_tag="AC162G_02950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR5074806.1"
                     /translation="MKYGLVLPYGDARSAANLAHEAEAAGWDGFFVCEPVWGIDAWVS
                     LAAAAMITKRIRLGTLLTPPSRMRPWKLASETVTLDHLSNGRVILSVGLGAIDTGFEA
                     FGEVTDRKTRAELLDEGLDILTGLWRGQPFNYGGKHYQIKGTRFMPPPPPVQQPRIPI
                     WVVGAWRREKSMRRVLKYDGLLPNVLDKNGKHRKMKPDDVSEMKAYVEANRTETTPFD
                     IVVEGTTPGDSPKKAASIVRKWADAGATWWIEALWNAPRDAKGMKIFRKRIEQGPPLV
                     RP"
     gene            complement(2086..3654)
                     /gene="metG"
                     /locus_tag="AC162G_02955"
     CDS             complement(2086..3654)
                     /gene="metG"
                     /locus_tag="AC162G_02955"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR5074807.1"
                     /translation="MTGGENTARRYYITTAIPYVNARPHIGHALEFVLADAVARWRRL
                     RGDEVRFLTGSDENSLKNVQAAEREGIPTQALVDRNAGLYYALRDSLSLSFDDFIRTS
                     VEPRHVDGVRKLWQACDRSGDIYKRAYRGLYCVGCEQFYAEDELVDGLCPDHLIRPEV
                     VEEENYFFHLSRYADRLIDAIDSDRLRIVPQTRKNEVLSFIRRGLVDFSISRSKARAH
                     GWGIPVPGDPDQVMYVWFDALGNYITALDYANEGEAYRRYWVSSPHRVHVIGKNIIRF
                     HAVYWPAMLLSAGVLLPTDILVHSFITIDGRKISKSLGNTIDPVELAQRYGVDALRYY
                     LLREVRTTSDGDFTLERFVQAYNADLADQLGNLLNRAVSMVGRYYRGVVPAPGELLPI
                     DEELIQPAKSLAERVDEALDRFAPHDALAAIWDAIGAANKYVDDTEPWALAKRRKTDP
                     SADVRLSTVLYNLVETLRLVAHYSLPFIPAAAESILRRLGLPSGVHEGWSDWGRYPAG
                     TAVQPGSVLFPKIE"
     gene            complement(4099..4947)
                     /locus_tag="AC162G_02960"
     CDS             complement(4099..4947)
                     /locus_tag="AC162G_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011044480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5074808.1"
                     /translation="MIPVNPLAGKVALVTGASRGVGKGIALGLGEAGATVYITGRTVE
                     EGKAAVDLPGTIHQTVHEVTQLGGKGSAIRCDHRNDQEVEAVFQRILTEESRLDILVN
                     NVWGGYEHFNDGTEFWNEKGFWTAPISRWDAMFQAGVRAHYVASVLAARIMIAQRAGL
                     IVNISFFAAHREDRGVAYGVAKAASDRMAACMAYELREHNVAAISLYPGLVRTESVMK
                     AARFFDLSNSESPQFIGRAVAALASDPAIMQKSGQTLVAAALALEYGFTDVDGRQPRP
                     ITVEEA"
     gene            complement(4919..6400)
                     /locus_tag="AC162G_02965"
     CDS             complement(4919..6400)
                     /locus_tag="AC162G_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIR5074809.1"
                     /translation="MTSLNAEVALQELIAGNQRYVANKLTHPHQTARHRQELIQRQEP
                     FAIILGCADSRVPPEIIFDQGLGDLFVVRVAGNVIDDAVLGSIEYATEHLKTPLLVVL
                     GHKACGAVKATLEALVAGEEGVGHMRSLVHVIQAAIAEARIQRGDFLDNAIRANVQRM
                     VKQLRFAEPILSSQVRDRKLKIVGAYYDLESGQVEIMGGGEMDIDSFWEYTDPALSEE
                     RFRTAMDTAQGDERLELLTQIARTYSLRKRFDEAHEVLNEVEQQLTHAGPRPRVRYLL
                     ERGRTFNSGGEKEKARMLCVEAWEQALAARQEGLAVDAAHMVAITYSGTRDAIEWNQR
                     GLVVARVSQDAKARALIPAMLNNSAWDLHDMGRFSEALSLFEEAQAEWIASGKPKQIQ
                     NAKWAVARCLRSLGRYDDALTIQRALEAEHSVAGTVDGYVFEEIAENLAGLGKLDEAK
                     PYFGKAVEELSKDERFVKNEAARLTNLKLRAIPEDDTRESSRR"
     gene            complement(6465..7118)
                     /locus_tag="AC162G_02970"
     CDS             complement(6465..7118)
                     /locus_tag="AC162G_02970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5074810.1"
                     /translation="MNCCTPNGLDKMFGESIARGDAKAYLNKGIDKRARLVVDALTAQ
                     GLSGSTVLDVGSGAGGLHIELLKSGAARATSVELSPAYVDAAREVAARTGFAEAVDHR
                     LLDFAQQAGEVDAADVVVMNRVVCCYPDMPALVRPAAEHARRLLALVFPRDVWWMRLS
                     GKMLNAWMALTRSAFRLYLHPPKAIVSTVKGAGMAPVFERLSGPWHVVVFRRGEPAA"
     gene            complement(7167..>8841)
                     /locus_tag="AC162G_02975"
     CDS             complement(7167..>8841)
                     /locus_tag="AC162G_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074811.1"
                     /translation="SAKRKTEIPIVTERDPRLADLRDVDEVGAEEWDEYDEGDAEDAI
                     SRRARESPTDVLRPYLDKQAKEQLVELIDELATSFPAVRKALQDRSELAEGTVQKLVK
                     SVRREIRELGSKPGWQNYWDGEGYTPDYSGVRDRLTVLLERGSFDEIVELGKELLESG
                     IRHVEMSHDEGETAAQIISCLNVVFRALSRSSLPSSGQALWAVEAELSDDYDLCRGAD
                     AFWEEANPSAADWNVVADKLLQRLKRLPEAEGDDSFSRNYRRDRLTDWLVHALQNAGR
                     EDEIIPLCRQEAEKTGSYVRLVSYLVDADRQEEAAQWIRKGIEATKVKWPGIAHQLRE
                     MQRELRAREKDWLRVAAFYAEDFFAQPTLQAYQEMQKAAERAEVWPAVRAAALHYLET
                     GELPKKTSAKGRPAAAWPLPETGLTEVSPRWRRQFPVVDTLIEVAIAEKRTEDVIRWY
                     DRRSADRTAWGWSASLDGKVARAVANTHPERAVAIWKHMAEDSIAQANKSAYQTAGAY
                     LRQVHRLLKQMGKEKEWQAYVAGLRQVNARKRRFLEVLDNLSGKRIIET"
BASE COUNT         1672 a         2812 c         2577 g         1780 t
ORIGIN      
        1 cctcaatgcg cgggccgtaa ctgagcacgt actccaccgc gccgtgttcc tcttcgaaca
       61 tcatgtccac ccgaatccaa ccgtccgcgt ccggcggata ttcgtgctcg atgcgcgcgt
      121 agcgcccttc ggcgcgcatg cgcggcagaa tgtgcggagc gacgcgcagg gcggcagggt
      181 agcgaggcag gttggccttg aactcggcgg acgattgctc ccagtacgcg gccaggtcga
      241 aatcgggtgg gcgaacgcat ggctcgtcgg tgagctgcgc atcctggaca cgcgagacgc
      301 ggtacgtgcg aaaatcgcct tcgacggcgg caaccaggta ccagatgctg cctttagcca
      361 ccagcccaag cggatcgacc acgcgctcga cggtcgagcc gtcgccgcgc ctgtaggtga
      421 tgcgcacctt gcgctcctgc cacacagctt cctggagtgc cggcagaaag tgaatcgctt
      481 cgtccggctg ccgccagccg cccgggtcga cgtgaattcg ttgtcgggcg tactccgcgc
      541 cacggcgtga catcgagggc agcgcagcca gcagcttaat gaacgctgcc tccgatgccc
      601 tgtccaggcc cagatcggcc agcaggcgcg gcggccggcc gagaaagagc gcctgaatct
      661 ccacctcgtt cagccccgtc aggttggtgc ggtagccatc cagaagtcgc cagccaccac
      721 ctattccgcg ttccgccacg accgggacgc ccgctgcgct cagtgcttcc atgtcacggt
      781 ggatagtccg ctcagacact tccaaccgtc gggcaagctc gcgcgccgtc aagcgctgat
      841 gcacctgcag cagcaacagg atcgataaca gacgatcggc gcgcatggga tggcctcgct
      901 ttctctatga cattctggac cggtaggtat tctaccagac aaatatgaca gccggtgtca
      961 tgaagtgtca actatactga gcgcaatagg acaaggaggg tctatgaaat acggattggt
     1021 gctaccgtat ggcgacgcac gctccgcggc caacctggct cacgaagctg aggcggccgg
     1081 ctgggacggc ttcttcgtct gcgagcctgt atggggcatc gacgcctggg tgtcgctggc
     1141 cgccgctgcg atgatcacga aacgcatccg gctcggcaca ctgctcacgc cgccgtcgcg
     1201 tatgcgtcct tggaagctgg ccagcgagac ggtgacgctc gaccatctat ctaacgggcg
     1261 tgtcatcctg tcggtcggtc tgggcgcgat cgacaccggg ttcgaggcat tcggcgaagt
     1321 caccgaccgc aagactcgcg ccgagctgtt ggacgaaggg ctggacatcc tcaccggcct
     1381 gtggcgcggc caaccgttca actacggcgg aaaacactac cagatcaagg ggacccggtt
     1441 catgccgccg ccgccacctg tgcagcagcc gcgcattccg atctgggtgg tgggcgcctg
     1501 gcggcgcgag aagtcgatgc ggcgcgtgct gaagtacgac ggcctgctgc cgaacgtgct
     1561 cgacaagaac ggcaagcacc gcaaaatgaa gccggacgat gtctccgaga tgaaggcata
     1621 cgtcgaggcg aatcgcacag agacgacgcc gttcgacatc gtcgtggaag gcacgacgcc
     1681 gggcgacagt ccaaagaagg cggcctcgat cgttcgcaag tgggcggacg ccggcgcgac
     1741 gtggtggatc gaggcgctgt ggaatgcgcc gcgcgatgcg aaaggaatga aaatcttccg
     1801 caagcgcatt gagcaggggc cacctctcgt tcgtccgtga tacaatccgc gtatcaccgt
     1861 cgtccggatc caagcgtgcc caaagatacg ctcacaacgc acgaagagtc cgcccgggac
     1921 gtgctcaccg ccgcgcaggt cgagccagtc gaagcgccgg tttccgctgc gcctgccgag
     1981 cgcagaaatt ctttcagggc atcctggggg cgcgcacggc gcaactcatc atgtgcgatt
     2041 gggttctctg agtagacctg gcaggtcttg gaagacctgc caggtctatt cgattttcgg
     2101 gaacagcacg ctgcccggtt gaaccgccgt gccggccgga tagcgccccc agtcgctcca
     2161 accttcatgc acgccggagg gcagacccaa ccggcgcagg atgctttcgg cagcggccgg
     2221 gataaaaggc aagctgtagt gcgcgaccag cctcagcgtc tcgaccaggt tgtacagcac
     2281 ggtcgagagc cgcacatcgg cggatggatc ggtcttgcgc cgtttcgcca gcgcccacgg
     2341 ctcggtatcg tccacgtact tgttcgccgc gccgatcgca tcccagatcg ccgccagcgc
     2401 gtcgtgcggc gcaaaacgat cgagcgcctc gtcgacgcgc tcggccagac tctttgccgg
     2461 ctggatcaac tcttcgtcga tcggcagcag ctcgcctggc gcgggcacga cgcccctata
     2521 gtagcgcccg accatgctga cggcgcgatt gagcaggttg ccgagctggt cggccaggtc
     2581 ggcgttgtag gcctggacga agcgctcgag cgtgaagtcg ccgtcgctcg tcgtgcgcac
     2641 ctcgcgcagc aggtaatagc gcagcgcgtc gacgccgtag cgctgcgcca gctcgaccgg
     2701 atcgattgtg ttccccagcg acttgctgat cttcctcccg tcgatggtga tgaagctgtg
     2761 aaccaggatg tcggtcggga gtagcacgcc cgccgagagc agcatcgccg gccagtacac
     2821 ggcgtgaaag cggatgatgt ttttgccgat gacgtgcacg cgatgtgggc tgctcaccca
     2881 atagcgccgg tacgcctctc cctcgttcgc gtaatccagt gccgtgatgt agttgcccag
     2941 cgcgtcgaac catacgtaca tcacctgatc cggatcgccc ggcacgggga tgccccagcc
     3001 atgtgcgcgg gccttcgagc gcgagatgct gaagtcgacc aggccgcgcc ggatgaagct
     3061 gagcacttcg ttcttccgcg tctgtggcac gatgcgcagc cggtcggaat cgatcgcgtc
     3121 gatcagccgg tcggcgtaac gtgacaggtg gaagaagtag ttttcctctt cgacgacctc
     3181 cgggcgaatc agatggtcgg ggcacagccc atcgacaagc tcgtcctcgg cgtagaattg
     3241 ctcgcagccg acgcagtaca gcccacgata ggcgcgcttg tatatgtcgc cgctgcgatc
     3301 acaagcctgc cacagcttgc gcacgccgtc gacatggcgc ggctcgacgc tggtgcggat
     3361 gaaatcgtcg aacgacaggc tgagtgagtc gcgcagtgcg taatacagcc ccgcgttgcg
     3421 atcgaccagc gcctgcgtgg ggatgccctc gcgctcggcg gcctgcacgt tcttcaggct
     3481 gttctcgtcg ctgccggtga ggaagcgcac ctcgtcgccc ctcaggcggc gccagcgcgc
     3541 cacagcgtcg gccagcacga attccagcgc gtggccgatg tgcggcctgg cgttgacgta
     3601 ggggatggcg gtggtaatgt agtagcgtcg tgctgtgttt tcgccgccgg tcacggtcgt
     3661 tctccttgct ggtaattggt gattggtaat tggaaattgg aggttggacg tgggaggttg
     3721 ggcgttggaa atggcctcgg acactgacca atctctaatt accaatctct caacaaaaaa
     3781 gggccgccca tgccgggaca gccccttgaa cttgcttgac gatacaatca ggtcgcaggc
     3841 agcacggaca cgggggtgcg ccacacgcgg cgcatccgca tggacatgct catcattcca
     3901 acagaaagcg gcacgatcac ggttgctgcc tcgatctcaa atcgccatca agtatagcgc
     3961 gatcacgtcg cgcgtcaagc gcctctatcc gttgtgtgca cactatccgt tgattcgttg
     4021 cctattcgtt gacgccatcc tgataactga acccaaaact cgcaaccgtg ataggacggt
     4081 caggtgcaac agatactgtc aggcttcttc caccgtgata ggtcttggtt gtctgccatc
     4141 gacatccgta aagccgtatt caagcgctag ggctgcggcc acaagagtct gccccgattt
     4201 ctgcataatc gctggatctg aggcaagtgc tgcaacggct cgcccaataa actgcggcga
     4261 ctccgagttg gataagtcaa agaaccgggc cgctttcatc acggactcag tgcggacaag
     4321 gccgggatac agggagatag cggctacgtt atgctcacgc aactcgtatg ccatgcacgc
     4381 cgccatgcgg tcggacgcgg ccttggcgac accataggca acgccccggt cctctctgtg
     4441 cgccgcgaaa aaggagatgt tgacaatcaa gcctgcccgc tgagcaatca taatccgtgc
     4501 agcgaggacg ctcgcaacgt agtgggcgcg aacgccggct tgaaacatgg cgtcccaacg
     4561 ggaaatggga gctgtccaga atcccttttc gttccagaac tctgtcccat cgttgaaatg
     4621 ttcatagccg ccccacacgt tgttgacgag aatgtctaaa cggctctctt cagtgaggat
     4681 gcgttgaaat acggcttcga cttcctgatc attacgatgg tcacaacgta tggcactgcc
     4741 cttgccgccg agttgggtga cttcatgcac ggtttgatga attgtgccgg ggaggtctac
     4801 ggcggccttg ccttcttcta ctgtcctgcc tgtaatgtag accgtggcgc cggcttcgcc
     4861 taagccaagt gcaatgcctt tgcccactcc gcggcttgcg ccagtcacca gggcaacttt
     4921 accggcgaga ggattcacgg gtatcatcct ccgggatagc acgtaacttc aaattcgtga
     4981 gtcgcgccgc ctcgttcttc acgaaccgtt cgtccttgct caattcttct acggccttcc
     5041 caaaataggg cttcgcctca tccagtttgc caagcccggc aaggttctcc gctatctctt
     5101 caaacacgta gccatccacc gtacctgcta cgctgtgttc ggcttcgagt gcgcgttgga
     5161 tggtcagtgc gtcgtcatag cgcccgagtg aacggagaca gcgcgcgacg gcccacttcg
     5221 cattctggat ttgtttgggc ttgccgcttg cgatccactc cgcctgcgct tcctcaaaca
     5281 aagataacgc ttcactgaag cgtcccatat cgtgcaaatc ccatgcgctg ttgttgagca
     5341 tcgccgggat cagggcacgt gcctttgcat cctgggacac gcgagccacg acaagcccgc
     5401 gctgattcca ctcaatggcg tcccgcgtgc ccgaatacgt gatggcgacc atgtgcgctg
     5461 cgtcgacagc cagaccttcc tggcgcgccg ccaatgcctg ttcccaggct tcgacacaga
     5521 gcatgcgcgc cttttctttt tcgccgccgg aattgaatgt tcggccacgc tcgagcaggt
     5581 accgaacgcg cggacgcgga cccgcgtggg tgagttgttg ttctacttcg ttcaacactt
     5641 cgtgggcttc gtcaaaacgc tttcgtagcg aataggtgcg agcgatctga gtgagcagct
     5701 caaggcgctc atcaccctgt gccgtatcca ttgccgtgcg aaaacgctct tcgctgagcg
     5761 caggatcggt gtattcccag aacgagtcga tatccatttc tcctccaccc attatctcca
     5821 cctgaccgct ttccaaatcg taataggccc caacaatctt cagttttctg tctcgaacct
     5881 gcgatgagag aatcggttcg gcaaatctca attgcttgac cattctctgc acgttcgcac
     5941 gaatggcatt gtccaggaag tcgccgcgct gaatgcgagc ctcggctatc gccgcttgga
     6001 taacatgaac caaagagcgc atatgcccta ccccttcttc gcccgcaacg agagcctcca
     6061 gggtagcttt caccgcacca catgctttgt gtcccaacac aaccagcaac ggcgttttca
     6121 agtgttccgt cgcgtattcg atactaccca ataccgcatc atcaatcaca ttgcccgcca
     6181 cccgcaccac aaacaaatcc ccgagcccct ggtcaaagat gatctccggc ggcacgcgtg
     6241 aatctgcaca gccaagaatg atggcaaagg gttcttggcg ttgaatgagt tcctgacgat
     6301 gtctcgccgt ctgatgagga tgtgtcaact tgttagcaac atagcgctga ttgccagcga
     6361 tgagctcctg caaggcaacc tcggcattta aggaggtcat cccttatctc ctgttgggcc
     6421 aacagagaca gtgaatctcc ttcggaaatc caactgacta aggctcacgc ggcaggctct
     6481 cctcgtcgaa acacgacgac gtgccacggc ccggacagtc tctcgaacac cggggccatc
     6541 ccggcgcctt tcacggtgga gacgattgct ttgggaggat gcaggtaaag ccggaaagcg
     6601 ctgcgcgtca gcgccatcca cgcgttgagc atcttgccgc tcagccgcat ccaccacacg
     6661 tcgcgtggga agacgagcgc cagcaggcgg cgcgcgtgct ccgccgccgg ccgcacgagc
     6721 gcaggcatgt ccgggtagca gcacaccaca cggttcatca cgacgacgtc cgctgcatcg
     6781 acctcgccgg cctgctgagc gaagtcgagc aggcggtggt cgaccgcttc ggcgaagccc
     6841 gtcctggccg ccacctctct cgccgcgtcg acgtatgccg gcgacagctc gaccgacgtg
     6901 gcgcgcgccg cgcccgactt gagcagctcg atgtgcagcc ccccggcgcc gctgcccacg
     6961 tccagcaccg tcgatcctga taggccttgc gctgtcagcg cgtctacgac gagccgtgcg
     7021 cgcttgtcga ttcccttatt gaggtaagct ttcgcgtcgc cccgcgcgat cgactcgccg
     7081 aacatcttgt cgagaccatt aggtgtgcag cagttcatcg tgattccccc gcagacattg
     7141 acaattcaga ctagactcaa agagtgctac gtttcaataa tgcgtttgcc cgacaggttg
     7201 tccaggactt ccaggaaccg tctcttgcgc gcattcacct ggcgaagccc ggccacgtag
     7261 gcttgccact ctttctcttt tcccatctgt ttgagcaggc ggtgcacctg tcgcagatag
     7321 gcgccggccg tctggtaggc gctcttgttc gcttgggcga tcgagtcttc ggccatgtgt
     7381 ttccagatgg ctaccgcgcg ctccgggtga gtattggcaa ctgcgcgtgc gaccttgccg
     7441 tcgagcgagg cgctccaacc ccaggcggtt cgatccgcgc tgcgccgatc ataccatcgg
     7501 atgacatctt ccgtgcgctt ctctgcgatg gcgacttcga tcaacgtatc gacgacagga
     7561 aattgccggc gccaacgagg cgatacttcc gtcaggccgg tctcaggtag gggccaggcg
     7621 gcggcagggc ggcctttagc agaagtcttc ttgggcagtt ccccggtctc cagatagtgc
     7681 agtgcggccg cccgcaccgc cggccagact tctgcccgtt ccgcagcttt ttgcatttcc
     7741 tggtaggcct gcagggtcgg ttgtgcgaaa aagtcctcgg cgtaaaacgc agcgacgcga
     7801 agccaatctt tttcgcgggc gcgcaactcc ctttgcattt cgcgcaactg gtgggcgatg
     7861 cccggccact taaccttcgt ggcttcgatg cctttgcgga tccattgcgc ggcttcttcc
     7921 tgacggtcag catcgaccag gtagctgacc agccggacgt agctgccggt cttctctgct
     7981 tcctgcctgc aaagagggat gatctcgtct tcgcgcccgg cattttgaag agcgtgaacc
     8041 agccaatcgg tcaggcgatc gcggcggtag ttgcgtgaaa aactgtcgtc gccttctgcc
     8101 tcgggcagcc gcttgagacg ctgcagcaac ttgtccgcga caacgttcca gtctgcggcc
     8161 gaggggttcg cctcctccca gaatgcgtct gcgccccggc acagatcgta atcgtcgctc
     8221 aactccgctt ccacagccca cagcgcctgt cctgaggatg gcagggacga acgcgacagc
     8281 gcccggaaga ccacgttcag gcacgagatg atctgcgcag ccgtttcccc ttcgtcgtga
     8341 ctcatctcga cgtgtcgtat gccggactcc agcaactcct tccccaactc gacgatttca
     8401 tcgaagctgc ctctttccaa aagcactgtc aggcgatctc tcacgcccga gtagtccggc
     8461 gtgtatccct cgccatccca atagttttgc cagccaggct tcgagccgag ttcccgtatc
     8521 tctctgcgca cggatttgac cagcttctga accgttcctt cggccagctc actgcgatct
     8581 tgcaaggctt ttcgcaccgc cgggaaactt gtcgccagct catctatcag ctcgacgagc
     8641 tgctctttgg cctgcttatc gaggtagggg cgcagcacat cggtagggga ctcgcgagcg
     8701 cggcgagaga tagcatcttc tgcatcccct tcgtcatatt cgtcccattc ctccgctccc
     8761 acttcatcaa catcccgcag atcagccagg cgcggatcgc gttctgtgac gatggggatc
     8821 tcggtctttc tcttcgcact c
//
LOCUS       DBNHXB010000076         7770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3033849, whole genome shotgun sequence.
ACCESSION   DBNHXB010000076 DBNHXB010000000
VERSION     DBNHXB010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3033849"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1634)
                     /locus_tag="AC162G_02980"
     CDS             complement(21..1634)
                     /locus_tag="AC162G_02980"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5074812.1"
                     /translation="MSRDLVGATLGKYRCEQFIGLGGMAEVYRAVDSELEREVALKVL
                     HPFFVSEEGFTERFRREARTLASLRHPNIVQVYDSGIQDFNSYLAMEYVPGPTLKDCL
                     RDLNMRNERMPLAEVRRIGDALLASLRYAHRANVVHRDLKPSNVILAGDGRVVLTDFG
                     LAKIVGSTIHTASMSMIGTPAYMAPEQAQAGTVDPRSDIYSVGVMLFEMLTGRLPFEA
                     DTPFAMIAKHAKETFPSAASVRRDLPRGLDRVLLIATAKDPSERFQSIDQFADALNAV
                     FEGRRLPFILPRLRLPRTARNWILGGAAAAIVLLGLGSFQGWFSAASPTLTPTITPSP
                     TPSFLAFIIGQTRLHEQPDLTSRVVGELSGGTLVELLVLQAPWWQVRSMDDAQLTGWV
                     LTVNLDLIPTPTPLPTLTNTPPPGASLTPTPTASATPRPSATPSATLTRSPTPTATPG
                     ESGPIGSPPATATPLARTPTRAPTQISTSPPSPTNPPPTPTRTPVPPTPTPAPPTATP
                     SVAPWTPTSTSRLPTVPGTSPTPTPCGGC"
     gene            complement(2067..2426)
                     /locus_tag="AC162G_02985"
     CDS             complement(2067..2426)
                     /locus_tag="AC162G_02985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR5074813.1"
                     /translation="MIVRLVCMKIKPEKLEEFRRVYEEACDRIRAFPGCMFLQLLLDT
                     EGEGNLYTISQWRSLDDLETYRSSAFFRGVWERLKPLFRERPWAQSTTVLVDSSKMKR
                     AAEAGAHEYPSIGTRPD"
     gene            complement(2564..3262)
                     /locus_tag="AC162G_02990"
     CDS             complement(2564..3262)
                     /locus_tag="AC162G_02990"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIR5074814.1"
                     /translation="MKVHLKIQRFNPEKDTRSWWSEHDVEVDETDRVLDALHLVKWYQ
                     DGTLTLRRSCAHGICGSDAIRINGRNALACKVLIKDVSKKITVQPLLGLPIIKDLVVD
                     MEPFFASYRSVKPYLVNDSDPGERERLQLPEERARYDDTTKCILCAACTTSCPMFWAT
                     GEYVGPAAIVQAHRFIFDSRDQIGDERLHILSDATGVWRCRTAFNCVEACPRGIDVTR
                     AIKEVQSAISTGKR"
     gene            complement(3281..5053)
                     /gene="sdhA"
                     /locus_tag="AC162G_02995"
     CDS             complement(3281..5053)
                     /gene="sdhA"
                     /locus_tag="AC162G_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HIR5074815.1"
                     /translation="MAKHHQFDAVIVGAGGAGLMAALHVSKQVNTAVLSKLYPTRSHT
                     GTAQGGIAAPLGNMEEDRWEWYMYDTIKGGDYLVDQDAAEILARESIDAIIELEHMGL
                     PFDRTPDGKIEQRRFGGHTRNYGEAPVMRSCKAADRTGHMILQTLYQQCIKQKVNFFD
                     EFQVIDLLIEDNVCRGVVAVEILTGEFHVFHAKAVLIAVGGFGRVWQVTSNAHSLTGD
                     SVATVFRRGLPLEDMEFFQFHPTGIYKMGVLLSEALRGEGAVFINDTGERFMERYAPT
                     MKDLAGRDVCSRAIYLEVRDGRGIGGKDYVYIDTRPETINRFQSGPGGKPVTAEHLEA
                     ALPDVFEMARIYLGVEPLKQGVPIQPTAHYAMGGIPTDNDGQVLADAKGAIVQGLYAA
                     GECACVSVHGANRLGTNSLVDLVVFGRRGGKAMAEFCKQAGFARLPADPEKRTLSELD
                     QIRSGSGKEKPAKIRDEMQKIMMQHVGVFREGPTLQKALDSVRALKERYARISIEDKS
                     AKFNTDLYEAWELGCLLDLAEVTTFAALDRTESRGAHYREDYPKRDDANWLKHSFVTR
                     KNGVLEVTYKPVTITKFEPQERKY"
     gene            complement(5249..6121)
                     /gene="sdhC"
                     /locus_tag="AC162G_03000"
     CDS             complement(5249..6121)
                     /gene="sdhC"
                     /locus_tag="AC162G_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HIR5074816.1"
                     /translation="MYRGGTGQWTWLLHRISGVAVVYFLTLHIFETLQLALGPVAYDE
                     ATAVYKQPWFRPFEFALVMAVVYHAFNGGRVMLFDYFPSLTRYRKQIFWIGVALFVVL
                     TPIIGYFMLSELFGMGLAEIFASMTGIGYASLIVGPLAIPVLYLLWRGSALSEGRRVI
                     LSASNSAPAPTASRFEKLAWWFMRISGIVLVVLVLLHLFIMHIERDIVEVTGEFVVDR
                     FLANPVWIAVDLSMLILAWLHGLNGVRVVLSDYMRRGAPRKVVLGAIGAFGLVWLLAG
                     GAVLFVIPTLVIGA"
     gene            complement(6218..>7770)
                     /locus_tag="AC162G_03005"
     CDS             complement(6218..>7770)
                     /locus_tag="AC162G_03005"
                     /inference="COORDINATES: protein motif:HMM:NF039189.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HIR5074817.1"
                     /translation="MSVTLAGAFILIALIVDQGFDAFRGNFIGASSGALALIIAGGAA
                     WRFYWQTVRRETGSAADERASGTRKLYLFGAMTLALLGVLALVQQVLSEFFVRLLDTG
                     LNGYKPWTPLIIAVLLGLIWRWHSQVAEADRAARADGTRGGDLRRGYWFVLAAYAIFT
                     AATGLIAFIAGLLSHLGGEPPFSFGILSQSWIQTLFPPLMQIVVSGVAIGLFWLPSQR
                     RAAAGDETERASLARQWLVHIVVFAATVWALSGVLGVLTDILSRLLQGPVDRLLVLTI
                     NGPLASLIVGGLLLLYFFRYVRSTLPSPRLSEYILAGVALFVGVLGVQQLVATLFRVL
                     GGQGPSIESIITFTLPPLLVGLAVWRWRWSAIERDAAGEDGSAARSYLWRKVHLYFYH
                     LTGLVMILTGAVIILGLIIGAILGQPLTGPFDGNVFSILADPLSLLLVGSGLLIYMMQ
                     VVNSDGRLGALSVEEVMRRTLGDSLPTWVIVLVITFVAGPFLMVISLAALGPAIGSIF
                     SNIVSGIE"
BASE COUNT         1528 a         2464 c         2438 g         1340 t
ORIGIN      
        1 gtcgaagtag gtggccagtg ctaacacccg ccgcagggcg tcggcgtggg agacgtaccg
       61 ggaacggtcg gcagacgcga agtcgacgtc ggcgtccatg gcgctacaga tggcgtagcg
      121 gtcggcggcg ccggcgtagg agtaggcggt acgggcgtcc gcgtgggcgt tggcggcgga
      181 tttgtcggtg acgggggcga agtcgaaatt tgagtcggcg ccctggtcgg cgttcgtgcc
      241 aacggggtcg ccgtagcagg cggcgagccg atcggaccgc tctccccggg cgtagcggtc
      301 ggcgtcggag aacgagtgag cgtcgcgctc ggcgtggcag acggtcgcgg cgtggcggat
      361 gccgtcggcg tcggcgtgag cgaggcaccg ggcggcggcg tattggtgag tgtgggcagg
      421 ggcgtcggcg ttgggatgag gtcgagattc accgtcagca cccagccggt gagctgagca
      481 tcgtccatcg aacgcacctg ccaccacggg gcctgcagga ccagcagctc gactaacgtt
      541 ccgcccgaaa gctcgcccac aacgcgggac gtgaggtcgg gctgttcgtg cagacgagtc
      601 tgcccgatga taaacgcgag aaaactcggc gtggggctgg gcgttatggt cggcgtcaaa
      661 gtgggcgagg cagcgctgaa ccaaccctgg aacgagccaa gtcccaggag gacgatggcc
      721 gcagcggctc cgccgagaat ccaattccgc gccgtgcgcg gcaggcgcag acgcggcagg
      781 atgaacggca ggcgtcgacc ttcgaacacg gcattcaagg catcggcgaa ctgatcgatc
      841 gactggaagc gctcggacgg atccttggcg gtggcgatga gcagcacccg atccaggccg
      901 cgcggcagat cgcggcgcac agaggcggcg cttgggaacg tctctttcgc gtgcttcgca
      961 atcatggcga agggcgtgtc cgcctcgaat ggcaaacggc cggtgagcat ctcgaagagc
     1021 atcacgccca ccgagtagat gtcgctgcgc ggatcgaccg tgcccgcctg cgcctgctcg
     1081 ggagccatgt acgccggcgt gccgatcatc gacatgctgg ccgtgtggat cgtcgagccg
     1141 acgatcttgg ccagcccgaa atcggtgagg acgacgcgcc catccccggc gaggatgacg
     1201 ttggagggct tcagatcgcg gtgcacgacg ttggcgcgat gcgcgtaacg cagcgacgcc
     1261 aggagcgcgt cgccgatgcg gcgcacttcg gcgagtggca tgcgctcgtt gcgcatgttc
     1321 agatcgcgga gacagtcttt gagcgtcggg ccaggcacgt actccatggc gagataggag
     1381 ttgaaatcct ggatgcccga gtcgtagacc tggacgatgt tgggatgccg gagtgaggcc
     1441 agcgtgcgcg cttcgcgacg gaagcgctct gtgaatcctt cttcgctgac gaagaacgga
     1501 tgcagaacct tgagcgccac ctcgcgctca agctcagaat ccacggcgcg atacacctct
     1561 gccatgccgc cgaggccgat aaactgctcg cagcgatatt taccgagagt ggcgccaacc
     1621 aggtcgcggc tcatactcgt atctaacccg gcgcgcctcg ccaggtgaga tcactctcac
     1681 caggcctgcc gtcagccagc cgggttatcc ttattcgata catattatag aacggctctg
     1741 cctatccgat caattggaaa tagtaggaag gtatgcctaa ggattctgcg caaaaccaca
     1801 ccaggcgccg cgagcgcccg ccatgtctta gctgtgtcca aagccgtaag cgccctgttc
     1861 gaaacgacca tttggctcta ttcttccaag atacagagcg ccggcgcgat tgccgagaac
     1921 atgagccagg aatggtggga gctgctcatg cacggcgtgt ggttcacttg cacgcagaaa
     1981 gagttgccgt atgaataccg gggagtatat cagatttcag gaatttgaag agactggaca
     2041 tgcgacccgc ccctagcatg tgagcgtcag tcgggccgcg tacctatcga cggatattcg
     2101 tgggcgccgg cttccgccgc ccgtttcatt ttcgaggaat ccaccagaac tgtcgtgctt
     2161 tgcgcccagg gtcgctcgcg gaacagcggt ttcagccgct cccacacgcc gcgaaagaat
     2221 gcgctgctgc gataggtttc caggtcgtcc agcgagcgcc attgactgat cgtatacagg
     2281 ttgccctcgc cctccgtatc aagcagtaat tgtagaaaca tgcagcccgg aaaggcccgg
     2341 atgcgatcgc aggcttcctc gtaaacgcgt cggaactcct ccagcttctc cggcttgatt
     2401 ttcatgcaca ccaggcgcac gatcatggcg gctcctccga atggcccgtg ggtcggcaat
     2461 tgtgagaaat acaacaccgc cccgcgcgat ccgtttcgca gacaaaacgg cctcgtctgc
     2521 ttgacgggac cggggtcgct caattgcgtc accggacggc ggcttatcgt ttgccggtgc
     2581 tgatcgcgct ctgaacctct ttgatcgcac gcgtcacgtc gatgccgcgt gggcaggcct
     2641 ccacgcagtt gaaggccgtg cggcagcgcc atacgcctgt cgcgtcggac aggatgtgca
     2701 ggcgctcgtc gccgatctgg tctcgactgt cgaagatgaa acggtgcgcc tggacaatcg
     2761 ccgccggccc cacatattcg ccggtggccc agaacatggg acacgacgtg gtgcacgccg
     2821 cgcacaaaat gcacttcgtc gtgtcgtcgt aacgggcgcg ctcctcaggt aactgcagcc
     2881 gctcgcgctc gccgggatcg ctatcgttga ccaggtaggg cttgacggag cgatacgacg
     2941 cgaagaacgg ctccatgtcc acgacgagat ccttgataat cggaaggccc agaagcggct
     3001 ggaccgtgat cttcttgctg acgtccttga tcagcacctt gcaagccagc gcgttgcgac
     3061 cgttgatgcg tatggcgtcc gaaccgcaaa tgccgtgcgc gcacgagcgg cgcagcgtca
     3121 gcgtaccgtc ttgataccac ttcaccaggt gcagggcgtc gagcacgcga tcggtctcgt
     3181 caacctcgac gtcgtgctca ctccaccacg acctagtgtc tttctcgggg ttgaagcgtt
     3241 ggattttgag gtgaactttc atattctctt ccgtgcgtcc ctaatacttc cgttcctgtg
     3301 gctcaaactt ggtgatcgtg accggcttat aggtcacttc tagcacgccg ttcttgcgcg
     3361 tcacaaagct gtgcttcagc cagttcgcat cgtcgcgttt ggggtagtct tctcgataat
     3421 gcgcgccccg gctctcggtg cggtcgagcg cggcgaaggt cgtcacttcg gccaggtcga
     3481 gcaggcagcc cagctcccac gcctcgtaca ggtcggtgtt gaacttggcg cttttatcct
     3541 cgatcgagat gcgcgcgtag cgctctttca gcgcccgcac actgtcgagc gccttctgca
     3601 gcgtcggccc ttcgcggaac acgccgacat gctgcatcat gatcttctgc atctcgtcgc
     3661 ggatcttcgc cggcttctct ttgccgctgc cgctgcggat ctgatcgagc tcggacaacg
     3721 tccgcttctc cgggtcggcg ggcaggcggg cgaagccggc ctgcttacag aactcggcca
     3781 tcgccttgcc gccgcgccgc ccgaacacca ccaggtcgac cagcgaattg gtgccgaggc
     3841 ggttcgcgcc gtgcaccgag acgcacgcgc actcgccggc ggcgtacagc ccctgcacga
     3901 tcgcgccctt cgcgtcggcc agcacttggc cgtcgttgtc ggtcgggatg ccgcccatcg
     3961 cgtaatgcgc ggtgggctgg atcgggacac cctgcttcaa cggctcgacg ccgagataaa
     4021 tgcgcgccat ttcgaagacg tcgggcaggg ccgcttccag gtgctcggcc gtcaccggct
     4081 tgccgcccgg tccgctttga aatctgttga ttgtctccgg acgcgtgtcg atgtacacgt
     4141 aatccttgcc gccaatgccg cgcccgtcgc ggacctccag gtagatcgcg cgcgagcaca
     4201 cgtcgcggcc ggccaggtct ttcatcgtcg gggcgtaacg ctccatgaag cgctcgcccg
     4261 tatcgttgat gaacaccgcg ccctcgccgc gcagcgcttc ggacagcagc acgcccatct
     4321 tgtagatgcc ggtcggatgg aattggaaga actccatgtc ctccaatggc aggccgcgcc
     4381 ggaagaccgt ggcgacgctg tctccggtga gcgagtgtgc attcgaggtg acttgccaca
     4441 cgcgcccgaa gccgccgacg gcgatcaata ccgccttggc gtggaagacg tgaaactcgc
     4501 cggtcaggat ttccacggcg acgacgcccc ggcacacgtt atcctcgatc agcagatcga
     4561 tgacctggaa ctcgtcgaag aagttgacct tctgcttgat gcactgctgg tacaacgtct
     4621 gcagaatcat gtggccggtg cgatcggccg ccttgcacga ccgcatcacc ggcgcttcgc
     4681 cgtaattccg ggtgtgtccg ccgaaacggc gctgctcgat cttgccgtcg ggcgtgcggt
     4741 cgaacggcag gcccatgtgc tccagctcga tgatggcgtc gatcgattcg cgggcgagga
     4801 tctcggccgc gtcctgatcg accagataat cgccgccctt gatcgtgtcg tacatgtacc
     4861 attcccagcg atcttcttcc atgttgccga gcggcgcggc gatgccgccc tgcgccgtgc
     4921 cggtgtgcga tcgcgtggga tacagcttgg agagaacggc ggtgttcacc tgtttcgaca
     4981 cgtgcagcgc ggccatgaga cccgcgccgc ccgcgccgac gatcacggca tcgaattgat
     5041 gatgttttgc catacactat gctccgggtg accggcgcct tcgaggggcc ggtcgcgcat
     5101 tgatgatcag tggagtttgg caacagccct atacggcgtt cggagtgcag gcttcagcct
     5161 gcggaagctc aagatatggc agcctgaaga gcctgccctg agcctgccga agggctacac
     5221 tccggcaatt tgccaacatc caggatgatt acgctccgat gaccagcgtc ggtatgacga
     5281 agagcaccgc gccgccggcg aggagccaca caagaccaaa tgcgccgatc gcgccgagca
     5341 caactttgcg cggcgcgccc cgccgcatgt aatcggacag cacgacgcgc acgccgttca
     5401 ggccatgcag ccaggccagg atcagcatcg agagatcgac ggcaatccag accggattgg
     5461 ccaggaaacg atcgacgacg aactcgccgg tgacctcaac gatgtcgcgc tcgatatgca
     5521 tgatgaacag gtggagcaac accaggacca ccagcacgat acccgagata cgcataaacc
     5581 accaggccag cttctcgaac cggctggcgg tgggcgcggg cgcgctgttc gacgccgaca
     5641 ggatcaccct gcggccctcg ctcagagccg agccgcgcca caacaggtac agcaccggaa
     5701 tcgccaacgg cccgacgatc agcgacgcgt agccgatgcc ggtcatgctg gcgaagatct
     5761 ccgccaggcc catgccgaac aattccgaca gcataaagta gccgatgatc ggcgtgagca
     5821 ccacaaacag ggcgaccccg atccagaata tttgtttgcg atagcgtgtg agagacggga
     5881 agtagtcaaa caacatcacg cggccgccgt tgaaggcgtg atacacgacg gccatcacca
     5941 gcgcgaactc gaagggtcga aaccacggct gtttgtaaac ggcggttgct tcgtcgtaag
     6001 ccacagggcc tagcgcgagc tgcagggttt caaagatgtg aagggtgagg aaatagacaa
     6061 cggcgacgcc actgatacgg tgcagcagcc aggtccactg gccggtacct cctcggtaca
     6121 cggtacgcct ccttgttcag gcgggaatag aaagcggagt gatgcgatta tatcgcaccc
     6181 cccctcgcca ggcaaaccga acgagtgcct tgctgtacta ctctatccca ctgacgatgt
     6241 tgctgaagat gctcccgatc gccgggccga gcgcagctag ggagatcacc atcaagaacg
     6301 gcccggccac gaaggtgatg acgaggacga tcacccacgt cggcagcgag tcgcccagcg
     6361 tgcgccgcat gacctcttcc acggagagtg cgccgaggcg gccatcgctg ttgacgacct
     6421 gcatcatgta gatcaagaga ccgctgccga caagcagcag cgacagggga tcggccagaa
     6481 tcgaaaacac gttgccgtca aacggcccgg tcaggggttg tcccaggatg gccccgatga
     6541 tgaggcccag gatgatcacc gcgccggtca ggatcatcac caggcccgtc aggtgataga
     6601 aatacaggtg gaccttgcgc cacaagtacg agcgcgcagc gctgccgtct tcgccggccg
     6661 catccctctc gatcgccgac cagcgccagc gccatacggc aaggccgacc agcagcggcg
     6721 gcagggtgaa ggtgatgatc gactcgatgc tcggtccctg cccacccagc acgcgaaaca
     6781 acgttgcgac gagctgttga acgcccagca cgccgacgaa cagtgcgacg cctgccagga
     6841 tgtactccga gaggcgcggc gagggcagcg tggagcgaac atacctgaag aagtacagca
     6901 gcagcaaccc accgacaatc agtgacgcca acggcccgtt gatcgtcagc acgagcagcc
     6961 ggtcgacagg gccttgcagc agccggctca gaatgtccgt cagcacgccc aacacgccgc
     7021 tcagcgccca caccgtcgcc gcaaacacga cgatgtgaac gagccactgc cgcgcaagcg
     7081 aggcgcgttc tgtctcgtcg cctgccgcag ctcgtctctg agagggcaac caaaatagcc
     7141 cgatcgccac gcccgacacg acgatctgca tgagcggcgg gaacaatgtc tggatccagc
     7201 tctggctcaa gattccgaac gagaacggag gctcgccacc gaggtgcgag agcaacccgg
     7261 cgatgaatgc gatcagtccg gtggccgccg tgaaaatggc atacgcagcc agcacgaacc
     7321 agtagccgcg ccgcaggtcg ccgccgcgcg tgccgtcggc cctggccgct cgatcggcct
     7381 cggcgacctg gctgtgccag cgccagatca gcccgagcag gactgcgata atgagcggcg
     7441 tccacggctt gtagccattc aacccggtat ccagcaggcg cacgaagaat tccgaaagca
     7501 cctgctgcac cagcgccaat acacccagca gcgccagcgt catcgcgccg aagagataca
     7561 gcttgcgcgt gccggacgcg cgctcgtcgg cagcggagcc agtttcacgc cgcacggtct
     7621 gccagtagaa gcgccacgcc gcgccgcccg cgatgatcaa cgccagcgcc ccggagctcg
     7681 ctccgatgaa attgcctctg aacgcgtcaa agccctgatc gacgatcagc gcgatcagga
     7741 tgaaagctcc ggccagcgtc acactcatac
//
LOCUS       DBNHXB010000077         8700 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1188882, whole genome shotgun sequence.
ACCESSION   DBNHXB010000077 DBNHXB010000000
VERSION     DBNHXB010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8700)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8700)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8700
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1188882"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1123
                     /locus_tag="AC162G_03010"
     CDS             152..1123
                     /locus_tag="AC162G_03010"
                     /inference="COORDINATES: protein motif:HMM:NF024919.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR5074818.1"
                     /translation="MSARTLLPVVLLMAVAVTPAQAQSGQVTHVVQTGENLFRIALRY
                     GLTADQVAQANGIRDPSRIYVGQVLVIPVPGSAVPPASAASSASNPAGFHVVQAGENL
                     YRIGLKYGLTAQQIATANGLSGVNMIFVGQRLTIPAPVSAPPQSAPAASGTRLAVPLY
                     GQLRTLSCEAAAARMLAEYYGVRQVEDWFQKAFGLSDNPHLGFRGNVDGEFGWIDDYG
                     VHAEPVARALQASGVGASARYGLTADDLRAALDRGAPVIVWTSPRTDTYDMPQGYRLV
                     PEEHTYVVVGYDASGFAVHDPLYGGRRLKLASIPGWELFGNMAVVGP"
     gene            1246..2538
                     /locus_tag="AC162G_03015"
     CDS             1246..2538
                     /locus_tag="AC162G_03015"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR5074819.1"
                     /translation="MARKPIILIRNGHIGTLRGLVKADILIRGDRIVAVEAGLTSPPG
                     ATLVEAKGKVILPGLIDAHVHLGDTGGAFKEDFYPETCAALAGGVTTALVMPDLRPPL
                     IDASSLAYALARAAQTAVCDHGLFLGATRGNLAAAARVKNAVGLKLYMGSRAGSPVVG
                     DFASQFEFFKAYPPTRVIALHAEDDAALQFFARQEKRCPPLCAELAVQRALAIAGELK
                     RRIHICHISTARELALVQDAKKHGVPVTCEISPHHLFLSTGVEKHLGPRGRMYPPLRP
                     DQDVRMLWGGLGSIDVVATDHTPRVLDEKRGDSASLGVPGLETMLPLLLTAVHQQQLS
                     YGDLVRLTADGPAKIFDLADKGRIQESAHADLVIVDPSVQWKIEGAKLRTGGGWTPFE
                     GWQVRGKVEQVYLRGQSVYDGERVLVEPGYGQRVKQMD"
     gene            complement(2598..4400)
                     /gene="lepA"
                     /locus_tag="AC162G_03020"
     CDS             complement(2598..4400)
                     /gene="lepA"
                     /locus_tag="AC162G_03020"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR5074820.1"
                     /translation="MDERHIRNFCIIAHIDHGKSTLADRLLEVTGTISEREMTSQVLD
                     SMDLEREKGVTIKASAVRMLHTSRSGERYELNLIDTPGHVDFTYEVSRALNACEGAIL
                     VVDASQGVEAQTLANLYLALDADLTIIPVVNKIDLPAARPGEVAAEIESVLGVPADEV
                     LRVSAKMGPGIDAVLEAIVERVPQPKGDPDAPPRALIFDSHYDPFKGVVAYVRIIDGT
                     LERKTRLRFMASKAETDPLELGIFAPNMKPGDRLEAGEVGYVATGLKTIRDCHVGDTI
                     TAVERPAPEPLPGYRPVKPMVFAGLYPVETDDYHELKDALEKLQLNDASLVYEPESSQ
                     ALNFGFRCGFLGLFHMEIIQERLEREYGLDIIATAPSVEYEVLLTSGAAIRIDSPAAL
                     PDPSSIEEIREPWMTVQVYTPAEHIGTVMDLVINRRGEQVSMEYLDPNRVVLTYRLPL
                     SEIIVDFHDQLKSRTRGYASMDYHFDEYRAGDLVKMDILVNEQPVDALALIVHKDKAY
                     EKGSAITRKMKEVIPSQLFPVPIQAAVGGRIIARTTVKAQRKDVLAKCYGGDVTRKRK
                     LLEKQKAGKKRMKMIGAVEVPQEAFMAILKLGEE"
     gene            complement(4476..5186)
                     /locus_tag="AC162G_03025"
     CDS             complement(4476..5186)
                     /locus_tag="AC162G_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074821.1"
                     /translation="MHNQVFRALVCVVLSGLALAGCGSATATPEPDRVATRVAEELAV
                     AATLTAAAPPATQPPPTDTSEPTAPPPPSDTPAPTQPPPPPTPTPEIDRVIPGAGTTN
                     GLLGVIALPGYTGLLEEPIIFQDRIVFHLQVFDPDTGNQANGAGIHTVDFFISDPNGE
                     SVHQRTEQNPLYCAFGGGDNGQPCNVWAFPEHGNTWTNGQAVVNDVCCYEVNMVVHTQ
                     DPEKDGANWRFSFIVRLP"
     gene            complement(5198..6748)
                     /locus_tag="AC162G_03030"
     CDS             complement(5198..6748)
                     /locus_tag="AC162G_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5074822.1"
                     /translation="MKRDLKKPERRRPSARGRQPPPAQNVEVSGGANIEADNVEIGGD
                     VVGRDKITNVISVTRSAIHIGTLAIPTLPVVIAAILVTAGLVFVGVSVIGPPGPSKMT
                     GGFNVAVAQFGQEAENGQVVPSEEGQRLSRTVFGVLREQTDEFSDIELRSQVQIWHDD
                     LANSEMGTDIGLIADEEAAQALAERLGADMVIYGAIDRQSNFVPKFYVTTEAKGAIDS
                     YVAGHQALGSRPIRVDPDTSLSAGTDIARRARALTYLTIGLTYDVLGRSEDALQVYRD
                     AETQLANWNETGEGKEVLYFFLGQAALYFSQQQAGLDQATELRQEAQRAFENGIRSNP
                     EYARPHVGLGSVYYLQAFDTQPLSTLLGASDVLTQTFAEYNQALELAKQSNDRQAEIL
                     ATYSLGTAQYLQGGAYKSDGQIDAADAAFRQAIDTLDSILASLKATNQQRLLGQAYQA
                     QGSAFKELARMWAEQGDSQKAGVLIEQARQALDQCIALSIDSQDKVLLELIIRGRCVP
                     QRASLDMP"
     gene            complement(6760..6837)
                     /locus_tag="AC162G_03035"
                     /pseudo
     CDS             complement(6760..6837)
                     /locus_tag="AC162G_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874900.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            complement(6899..>8700)
                     /locus_tag="AC162G_03040"
     CDS             complement(6899..>8700)
                     /locus_tag="AC162G_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074823.1"
                     /translation="GLRIARGGCEVPRNPDVPLGADQADRIVEAVNGVQPGGEVALVE
                     SIRAAADDLPPDPQGQNTNTIIVISAGQDRCLESDPCRAMASVAQSLERANIDFQLEI
                     VALKPDEQTRLQLTCLVRANVNGRFHEANSVNDLRTIFQEIEPAQAVPGVQNGSFESG
                     DAEAWSGEGIEVVAVEDADVPDVGQYAAQMKPGQALEQTAKVPGVGQPQLTVWYRAPG
                     EKAGGVLKIYVNGQLEYTDDSPADRTGNYVAADAPDWLVAVVPAEGFAGQEVTLRIEY
                     AEAASRAGGVLRFPARQVDDVLWVDNVAFVAEGSLPAVIEATPTPSATPALRPTPGPD
                     ASQTPTLLRTATLTPTPPVSVTATLTRTPAAQPLTFTWRAGAFRESGRNDQGVGIWAQ
                     EIFVEARGGVPPYTILFDNAPKTTMPFDVFGLYCIGQVGTITVRSADGQVASQRVTIR
                     APLCPTNTPTPTATRTRTPTASHTPTPLPTTDDISNFSVVSATDTEIQVTVDYAYIPA
                     HGADVWLGVWVLSDGEFQIYYGYAPALLSSNSGRATVRVTYGYNDPPASLITDQIEVI
                     MYTGDGKVFYSETFDYLKTWSLAPPLTPASSSR"
BASE COUNT         1440 a         2736 c         2840 g         1684 t
ORIGIN      
        1 agaagaaggt gtcgagtata cagttgacac gccgtcagac gactatcccg atgagctatc
       61 catctaacca tccagccaac cattcaacca accaaccagc taacaagcgg acctattgcg
      121 cgattccgcg cccttggcta caattcgggc catgagtgcc cggacgcttt tgcccgtcgt
      181 gcttcttatg gccgttgctg tgacgcccgc gcaggcgcag agcgggcagg tcacgcacgt
      241 cgtccagacg ggtgagaacc tgttccgcat tgcgctgcgc tacgggctga ccgccgatca
      301 ggtcgcccag gccaacggca tccgtgatcc gtcgcgcatc tacgtggggc aggtcttggt
      361 catcccggtc cccgggtcgg ccgtgccgcc cgcctcggcg gcgagcagcg cctcgaaccc
      421 ggcaggattc cacgtcgtgc aggcaggaga gaacctgtat cgcattggac tgaaatacgg
      481 cctcacggcg cagcagatcg cgacggccaa cggcctgagc ggtgtcaaca tgatcttcgt
      541 cggccagcgc ctgacgattc ccgcgccggt cagcgcgccg ccgcagtcgg cgcctgcggc
      601 aagcggcacg cggctggcgg ttccactcta cgggcagttg cggacgctgt cgtgcgaggc
      661 ggctgcggcg cgcatgctgg ccgagtacta cggcgttcgc caggtcgaag actggttcca
      721 gaaggcgttt ggcctgagcg acaatccgca tctcggcttt cgaggcaacg tggacggcga
      781 attcggctgg atcgacgact acggcgtgca cgccgaacct gtggcgcgcg ccttgcaggc
      841 gtcgggcgtc ggcgcgtccg cccgctatgg cctgacagcc gacgacttgc gcgcagcgct
      901 cgatcgaggc gcgccggtca tcgtctggac ctcgccgcgc accgacacgt acgacatgcc
      961 ccaggggtat cggctcgtgc ccgaagagca cacgtacgtc gtcgtcggct acgacgcttc
     1021 gggcttcgcc gtccacgatc cgctctacgg aggccggcgc ttgaagctgg cgtcgatccc
     1081 cggctgggaa ctgttcggca acatggcggt cgtcggtccg tgaacggacg aatgaccgac
     1141 gtagacctgg caggtcttcg cgacccggcc tacggctcgc atcggcgaac aagacctgcc
     1201 aggtctatgg ctcttgattt tcaaatacag gcggtgtagg aagtcatggc ccggaagcct
     1261 atcatcctga tccgcaacgg ccatattggc acgctgcgcg gcctggtgaa ggccgacatt
     1321 ctgatacgcg gggatcgcat cgtggccgtc gaagcgggat tgacatcgcc gcccggcgcg
     1381 acgctggtgg aagccaaggg gaaggtcatt ctgccggggt tgatcgacgc gcacgttcac
     1441 ctgggcgata ccggcggcgc cttcaaagag gatttctatc ccgagacgtg cgccgcgctg
     1501 gccggcggcg tgacgaccgc cctggtgatg cccgacctgc gaccgccttt gatcgatgcg
     1561 tcgagtctcg cgtacgcgtt agcgcgcgcc gcccagacgg ccgtgtgcga tcacgggcta
     1621 ttccttggtg cgacgcgtgg caatctcgcc gcggccgccc gggtgaagaa cgcggtcggc
     1681 ttgaagctgt acatggggtc gagggccggc tcgccagtcg tcggcgattt tgcctctcag
     1741 ttcgagtttt tcaaggcgta tccgcccacc cgggtcatcg cgctgcatgc ggaagacgac
     1801 gcggcgctgc agtttttcgc caggcaggag aagcgctgcc cgccgctgtg cgccgagctg
     1861 gcggtgcagc gcgcgctcgc catagcaggt gagctcaaac gccgcatcca tatttgtcac
     1921 atctcgaccg cgcgcgagct ggcgctggtg caggacgcga agaagcacgg cgtgcctgtg
     1981 acgtgcgaga tctcgccgca tcatctgttc ctctcgaccg gggtcgaaaa gcatctcggc
     2041 ccgcgcggcc ggatgtatcc gccgctgcgc ccggatcaag atgtgcgtat gctgtggggc
     2101 ggcctcggct cgatcgacgt cgtcgcgacg gatcacacgc cgcgcgtgtt ggatgaaaag
     2161 cgcggcgatt cggcgtctct cggcgtcccc ggcctcgaaa cgatgctgcc cctgctgctc
     2221 accgccgtgc atcaacagca gttgtcgtac ggcgacctcg tgcgcctgac ggccgacggc
     2281 ccggcgaaga tcttcgatct cgccgacaag ggtcgcatac aagaaagcgc gcacgccgat
     2341 ctggtgatcg tcgatccgag cgtgcagtgg aagatcgagg gcgcgaagct gcgcaccggc
     2401 ggcggctgga cgccgttcga aggctggcag gtgaggggga aggtcgagca ggtctatctg
     2461 cgcggccagt cggtctacga cggcgagcgg gtgttagtcg agccggggta tgggcagaga
     2521 gtgaagcaga tggattgatg cgtgtaacgt gaaacgtaac gccttacgtt tcacgcttca
     2581 cgcatcacgc ttcacgttta ctcctccccc agcttcaaaa tcgccatgaa cgcctcttgc
     2641 ggcacttcga cggcaccgat catcttcatg cgcttcttgc ccgctttctg cttctctagc
     2701 agcttgcgct tgcgggtgac gtcgccgccg tagcacttgg cgagcacgtc tttgcgctgc
     2761 gccttgaccg tcgtgcgcgc gatgatccgc ccgccgacgg cggcctggat cggcaccggg
     2821 aacaactgcg acgggatcac ctctttcatc ttgcgcgtga ttgcgctgcc cttctcgtat
     2881 gccttgtcct tgtggacgat caaagccagc gcgtcgaccg gctgctcgtt gaccaggatg
     2941 tccatcttga ctaggtcgcc cgcgcggtac tcgtcgaagt gatagtccat cgaggcgtag
     3001 cctcgcgtgc ggctcttaag ctgatcatgg aagtcgacga tgatctccga cagcggcagg
     3061 cggtaggtca gtacgacgcg gttcggatcg aggtattcca tgctgacctg ctcgccgcgc
     3121 cggttgatca ccaggtccat caccgtgccg atgtgttcgg cgggggtgta cacctgcacc
     3181 gtcatccacg gctctcgaat ttcttcgatc gacgatgggt cgggcagcgc ggcgggcgaa
     3241 tcgatgcgga tggcagcgcc gctcgtcagc agcacttcgt actcgacgct gggcgcggtg
     3301 gcgatgatgt ccaacccgta ttcgcgctcc agccgctcct ggatgatctc catgtggaag
     3361 aggccgagga agccgcagcg gaagccgaag ttgagcgcct gcgacgactc cggctcgtac
     3421 acgagcgagg cgtcgttgag ctgaagcttc tccagcgcgt ccttcagctc gtggtagtcg
     3481 tcggtctcga ccgggtacag gccggcgaac accatcggct tgacgggccg gtaaccgggg
     3541 agcggctccg gcgcgggccg ttcgacggcg gtgatcgtgt cgcccacgtg gcagtcgcgg
     3601 atcgtcttca gcccggtggc gacgtagccg acctcacccg cttcgagccg gtcgccaggc
     3661 ttcatgttgg gggcaaaaat acccagctcc agcggatcag tctccgcctt gctcgccatg
     3721 aagcgcaggc gcgtcttgcg ctcgagcgtg ccgtctataa tgcgcacgta ggcgacgacg
     3781 cccttgaacg ggtcgtagtg gctgtcgaag atcagcgcgc gcggcggtgc gtcgggatcg
     3841 cccttcggct gcggcacgcg ctcgacgatc gcttcgagca ccgcgtcgat cccgggtccc
     3901 atcttggccg acacgcgcag cacttcatcg gcgggcacgc ccaggacgct ctcgatctcg
     3961 gcggcgactt cgccgggccg cgcggcgggc aggtcgatct tattgacgac ggggatgatc
     4021 gtcaggtcgg cgtcgagcgc gagatataga ttggcgagcg tctgcgcctc gacaccctgc
     4081 gaggcgtcga cgacgaggat cgcgccctcg cacgcgttca gcgcgcgcga aacttcgtac
     4141 gtgaagtcta catggcccgg ggtgtctatg aggtttagct cgtagcgttc acccgaccgc
     4201 gaggtgtgca gcatgcgcac ggcggaggcc ttgatcgtca cgcccttctc gcgctcgagg
     4261 tccatcgagt cgagcacctg cgaggtcatc tcgcgctcgg agatggtgcc ggtcacttcg
     4321 agcagccggt cggcgagggt cgatttgccg tgatcgatgt gggcgatgat gcagaagttg
     4381 cggatgtgcc gctcgtccat ctcgcggcga ttataccagc aaaaaagcct ggcgaaggtt
     4441 tagcaacttc gccaggcttt cctcgatggg aacagtcagg gcagccgcac aatgaaacta
     4501 aaccgccagt ttgccccgtc cttctccggg tcttgcgtgt ggacgaccat gttcacttcg
     4561 tagcagcaca cgtcgttgac gacggcctgc ccgttggtcc acgtattgcc gtgctcgggg
     4621 aacgcccata cgttgcacgg ctggccgtta tcgcctcccc cgaaggcgca atatagaggg
     4681 ttttgctcgg tgcgctgatg cacgctctcg ccgtttgggt cggagatgaa aaaatcgacg
     4741 gtatggatgc ccgcgccgtt tgcctggttg cccgtgtctg ggtcaaagac ctggaggtgg
     4801 aaaacgatcc ggtcctggaa aatgatcggc tcttccaaga ggcccgtata accgggcagc
     4861 gcaatcacac ccagcagccc gttggtcgtc ccggcgccgg ggatgacacg gtcgatttct
     4921 ggcgtcgggg tgggtggggg aggcggttgc gtcggcgcag gcgtatccga tggtggcgga
     4981 ggcgcagttg gctcgctcgt gtcagtggga gggggctgcg tcgcaggcgg agcggcggcg
     5041 gtcagcgtcg cggcgacggc gagttcttcg gccacgcgcg tcgccacgcg atcgggttcc
     5101 ggcgtggccg tggctgagcc gcatccggcc agcgccagac cggataaaac cacgcacact
     5161 agagcgcgaa agacctgatt gtgcattttc atgatcctta cggcatgtcc aggctggcgc
     5221 gctggggtac gcatcggccg cggatgatca gctcaagcag cactttatcc tgtgaatcga
     5281 tactgagcgc aatgcactgg tcgagcgcct gacgcgcctg ctcgattagt acgccagcct
     5341 tctggctatc gccttgttcg gcccacatcc tggccagttc cttaaaagct gagccctgcg
     5401 cctgataggc ctgtccgaga aggcgctgct ggtttgtcgc tttcaaggac gcgagaatcg
     5461 aatcgagcgt atcgatagcc tggcggaagg cggcatcggc ggcgtcgatc tggccgtcac
     5521 ttttgtacgc tccaccttgc agatattgag ccgtgccgag cgaataagtc gccaggattt
     5581 cggcttgtcg atcattggac tgcttcgcca gttccagcgc ctgattatac tccgcgaaag
     5641 tttgggtcaa tacatcggat gcaccaagga gtgtgcttaa tggctgcgtg tcgaatgcct
     5701 gcaagtaata gacgctgccc aagccgacgt gtgggcgagc gtactccgga ttgctgcgga
     5761 tgccattttc aaaggcgcgc tgtgcctcct gccgcagctc ggtcgcctga tcgagacctg
     5821 cctgctgctg tgaaaaatac aaggcagcct ggccgagaaa gaaatacagc acctctttgc
     5881 cttcgcccgt ttcgttccag ttggcgagct gcgtctcggc gtcgcggtag acctgcagcg
     5941 cgtcttcgga tcggccgagc acgtcgtaag tcagcccgat ggtcagatag gtcagcgccc
     6001 tggcccggcg ggcgatatcg gtgcccgcgc tgaggctggt gtccggatcg actcggatcg
     6061 gccgactgcc gagcgcctga tgcccggcga catatgaatc gattgcgcct ttggcttcgg
     6121 tcgtgacgta gaacttgggg acgaaattgc tctggcgatc gatcgcgccg tagatcacca
     6181 tgtccgcgcc gaggcgctcg gccagcgcct gagccgcttc ttcatccgcg atcaggccga
     6241 tgtcggtacc catctcggaa ttggcgagat catcgtgcca gatctgcacc tggctgcgga
     6301 gctcgatgtc gctgaattca tccgtctgct cgcgcagcac cccgaacacg gttcggctca
     6361 agcgctggcc ctcctctgac ggaacgactt gcccgttctc ggcctcttgg ccgaactggg
     6421 cgaccgcgac gttaaatccc cccgtcatct tcgatggccc cggcggcccg atcacactca
     6481 cgccgacgaa gaccaatccg gccgtcacca gaatcgcggc gatgacgacg gggagggtcg
     6541 ggatcgccag cgtgccgatg tgaatggcgc tccgcgtgac cgatatgacg ttggtgatct
     6601 tatcgcgccc gacgacgtcg ccgccgattt cgacgttgtc ggcttcgatg ttcgcgccgc
     6661 cgctgacttc gacgttctgt gctgggggtg gttgccgacc gcgcgccgag ggcctgcggc
     6721 gctccggctt cttcaaatcg cgcttcatcg atcgactccc agccggtcgg ttagcgtcga
     6781 tttgccgtgg tcgatgtggg cgatgatgca gaagttgcgg atgtgccgct cgtccatctc
     6841 gcggcgatta taccatttca gacctccgaa gtctccaaga cttcggaagt ctgaaacttc
     6901 aacgagaact ggaagcaggc gtcaggggcg gggccaaaga ccacgtcttg aggtagtcga
     6961 atgtctccga gtaaaagacc tttccgtcgc cagtgtacat gatgacttct atctggtctg
     7021 tgatgagact ggccggcggg tcgttgtagc catacgtgac acgcaccgtc gcccggccgc
     7081 tgttgctgga caggagtgca ggagcatacc cataataaat ctgaaactct ccatcactca
     7141 gcacccatac tccaagccag acatccgctc catgcgcagg gatataggcg taatccaccg
     7201 tgacctgaat ctctgtgtcc gtggcgctga ccacgctgaa gttgctgatg tcgtcggttg
     7261 tcgggagtgg cgtcggcgta tgggaggcag tcggtgttcg cgtcctggtg gcagttggtg
     7321 ttggcgtgtt tgtcgggcac agcggcgcgc gaatcgtcac tcgctggctt gccacctggc
     7381 cgtcggccga tcgaacggtg atcgtcccca cctggccgat gcagtacaga ccgaatacgt
     7441 cgaacggcat ggtcgtcttc ggagcgttgt cgaacaggat ggtgtagggc ggaacgccgc
     7501 cgcgcgcctc gacgaagatc tcctgcgccc agatgccgac gccctggtcg ttccgccccg
     7561 actcccgaaa cgcacccgca cgccaggtga aggtgagcgg ctgcgccgcg ggcgttcgtg
     7621 ttagtgtcgc cgtcacggag acgggcggcg tcggcgtgag ggtcgccgtg cgcagcagcg
     7681 tgggggtctg agatgcgtcg ggtccgggcg tcggcctcaa tgccggcgtg gcggatgggg
     7741 taggggtggc ctcgatcacc gccggcaggg acccctcggc gacgaaggcg acgttgtcca
     7801 cccacagcac atcgtcgacc tggcgtgccg gaaatcgaag cacgcccccg gcgcgagacg
     7861 cggcctcggc gtactcgatg cgcagcgtca cctcttgccc ggcgaagcct tccgcgggga
     7921 cgacggcgac gagccagtcg ggcgcatcgg ccgcgacata gttccctgtg cgatcggccg
     7981 ggctgtcgtc ggtgtactcc aactggccgt tgacgtagat cttgagcacg ccgccggcct
     8041 tctcgcccgg cgcgcggtac cacacggtca attgcggctg tccgacgccc ggcactttcg
     8101 cagtttgctc gagtgcctgc ccgggcttca tttgcgccgc gtactggccg acatccggca
     8161 cgtccgcatc ttcgacggcc acgacttcga tgccctcgcc gctccaggcc tctgcgtcgc
     8221 ccgactcgaa ggagccgttt tgcacgccgg gaaccgcctg cgcaggttcg atctcctgga
     8281 agatcgtccg aaggtcgttg accgagttcg cttcgtggaa acggccgttg acgttcgcgc
     8341 gcaccaggca ggtgagctgc agacgcgtct gctcgtcggg cttcagcgcg acgatttcca
     8401 actggaagtc gatgttggcc cgttccagac tctgtgcgac ggatgccatc gcccggcacg
     8461 gatcgctctc caggcacctg tcttgcccgg cgctgatcac gatgatcgtg tttgtgttct
     8521 gtccctgggg gtcaggcggc aggtcgtcgg ctgcggcacg gatcgattcg acgagcgcga
     8581 cttcgccgcc cggctgcacg ccattcacag cctcgacgat ccggtccgcc tgatcggcgc
     8641 caagcggcac gtcgggattg cgcggcactt cgcagccgcc tctggcgata cgcaagccga
//
LOCUS       DBNHXB010000078         5997 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3872531, whole genome shotgun sequence.
ACCESSION   DBNHXB010000078 DBNHXB010000000
VERSION     DBNHXB010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3872531"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..583
                     /locus_tag="AC162G_03045"
     CDS             221..583
                     /locus_tag="AC162G_03045"
                     /inference="COORDINATES: protein motif:HMM:NF021560.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HIR5074824.1"
                     /translation="MNAAKINYDEEADVLYVSFSRSEHVTGVELADNILLRLDTGKVT
                     GNAPRAVGLTFISFSRLMEHYRSRPLSVPLANLRSLPEDLWQAVLTVITTPPVSDFLA
                     VGLSLSPQVPPLPERVAA"
     gene            complement(698..1615)
                     /locus_tag="AC162G_03050"
     CDS             complement(698..1615)
                     /locus_tag="AC162G_03050"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR5074825.1"
                     /translation="MTDETKATIAQTLPPGGTRMYQEYGVEEDTARSAYHYEQDPEFY
                     YLQTAGEWNVYSCLMWEDGFSVTQAQEKKLDKLAECMQLKPGMHILDVGCGWGGPLVY
                     LCNTYGVTGHGITVSPKQAQAAQARAARYGVDATFEVVHWKNLPEVETYDAIYSDEVI
                     VHFFDLGGFFARCWKLLRTNGLMAHKELHWRHASYADVTPLGDHIQKVFGFTGNYIPL
                     HKELRLLEENVFKLEGVFEISMDDYQKTIDMWLKNVFDHRERMKALAGESFYKEYRAY
                     LKAARRFMASEVYLLDIVTSRKIAETLRR"
     gene            complement(1797..5651)
                     /locus_tag="AC162G_03055"
     CDS             complement(1797..5651)
                     /locus_tag="AC162G_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5074826.1"
                     /translation="MSALLETLASYVPSLVVRWYAEHPDPIITPGAERFPSAIFFADI
                     TGFTALTERLARLGPAGAEEVSRLLNDYFGQLIERVDAHGGDVVKFAGDALLALWPAV
                     DESLAAAVRRAAQCGLAAQAALHDYAAARDVRLSLRVGIGAGEMTSAHVGGVNGRWEF
                     VATGAPLSQIGAIKTEALPGDVIVSPEAWSLAGQWFTGHALPTGSMRLESARDSLPPR
                     RALRPALKAEMELPLRAYIPGAILERLAAGQSEWLAELRRVTVIFVNLPGQDDKAPRG
                     LKQAQMITRALQSVLYRYEASIDKISVDDTGANLVAALGLPPWTHEDDAARGAQAALA
                     IQSLLRELGLRPAIGVAGGLAFCGSVGTAARREYTMVGDVVNLAARLMQSARDDILCD
                     AATAQAAHAQVEFESLPAIRVKGKAEPVAIYRPIRAARATARPHVTMIGRAAEWAMLS
                     ERMQNLLHGEPGGTVVLEGEAGIGKSRLVDEALRRAQELGLACLLGAGDAIEKNTPYH
                     AWRDIFVKLLGLHAETDPENRREQILDRLRHDPQVASLAPLLNVALPVDLPENETTAS
                     MTDQARGDAARDVLLRLLNDLPRSEGMPDDRRPKVIVLEDAHWLDSASWALALLVSRR
                     LERSLLIVTARPMSDPPPPEYLQLMQSPNTRRLRLEALPAEDTLALVCQRLNVRSLPE
                     PVADLIREKAEGHPFFSEEVAYALRDAGLIEIAGGECRVAPDAGDLRAIAFSDTIQGV
                     ITSRIDRLTPPQQMTVKAASVIGRMFPFRILHDIYPIDSDRPYLAGYLDALQRLNLTL
                     LDTPEPDLAYIFKHVITQDVAYNLMLFAQRRQLHRAVAEWYERAQARERGRARYDQLL
                     AYHWSLAGDDEKAAGYLEKAAHQAMRSGANAEAKALFEQALETLSRLPVTPERQRQVV
                     DLAVDLARVGAYLPSDSILEALKRAQNAAESLGDEVRLAQVYGGAGAFHYVMGRLNEA
                     LDSFGKCMALAEKLGLEGLLMQPYNVVGRAVAVSGDFARAAGLLAKGIALAERYRDVE
                     LLAGSLAFYASTFWFQGERAEGTPHAERGLALAEELERPSRIAGNLMTMGFYHCFCGF
                     FDEAAAYLKRCLSIAEPKQYVQPLYIAYGCMGYLHLQEGDLARARRYLDDCLRLAETN
                     KASAFTPMYQAYRAEIDVIDGDWTAALARAQAAVAAAERSRQRMSLGEAQRILGRIYA
                     RAATPDWEKAEQAIKTSIALQQECNALPLVAVSTLDLGTLYRDRGLTDQARAAIGEAI
                     AMFERLEMAWHLDRARHIVASL"
     gene            complement(5799..>5997)
                     /locus_tag="AC162G_03060"
     CDS             complement(5799..>5997)
                     /locus_tag="AC162G_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR5074827.1"
                     /translation="RVVYDVGDWDQCVAVLPGGPSGHPVSPHYFDQFPLWLAGEARPM
                     PWSREAVEREAESHMRLVPSK"
BASE COUNT          996 a         1998 c         1938 g         1065 t
ORIGIN      
        1 cgccggctgc tatacgggcg tggtgaggcc acctgcattg ccactgccca taatgccagg
       61 cggcccggcg tccttggtca gatcgcaaat gatcgggctg cgcgcgaagt cgaggaaacc
      121 tgcttcgtcg gctgggggat agtcacgcgc cattggtcat gaccgcttgg ggtgcacccc
      181 aagtgctcag tctcgtcaaa gtggtatata atcaactgca atgaacgcag cgaagatcaa
      241 ctacgacgaa gaggcggatg tgctgtacgt gtccttttcg cggagcgagc atgttaccgg
      301 tgtcgaactg gcagacaaca tcctgctgcg actggacacg ggcaaggtaa ctggaaatgc
      361 gccgcgagcg gtcggcttga ctttcatcag cttctcgcga ttaatggagc attaccgctc
      421 caggccactc agcgtgccgc ttgcgaacct tcgcagtctg ccggaagacc tctggcaggc
      481 ggtcttgacc gttatcacaa ctccgccggt aagcgacttt ctggcagtcg ggctgtctct
      541 atcgcctcaa gttccccctt tgccggagcg agtagccgcg taagcacatg acgtcggcaa
      601 cgcaagcact cgtgtcgtgc cagtggcccg acagatcaaa atcggcggtc gcagaccccc
      661 gacgagcggg gacctccttt cagacttccg aagcctctca gcgcctcagc gtctcggcga
      721 tcttccgcga cgtgacgata tccagcaggt agacctcgga cgccatgaac cggcgcgcgg
      781 ctttgagata ggcgcggtat tctttgtaga acgactcgcc ggccagggcc ttcatgcgct
      841 cgcgatggtc gaagacgttc ttgagccaca tgtcgatcgt cttctgatag tcatccatag
      901 agatctcgaa gacaccttcg agcttgaaga cgttctcttc gagcaggcgc agctccttgt
      961 ggagcgggat gtaattgccg gtgaagccga agaccttctg gatgtgatcg cccagcggtg
     1021 tgacgtcggc gtaggaggca tgccgccagt gcaattcctt gtgcgccatc agcccgttcg
     1081 ttcggagcag cttccagcac ctggcgaaga acccgcccag gtcgaagaag tggacgatca
     1141 cctcgtctga gtagatcgcg tcgtacgttt cgacttcggg caggttcttc cagtgcacga
     1201 cttcgaaggt cgcgtcgacg ccgtagcgcg cggcgcgcgc ctgtgcagct tgcgcctgct
     1261 tgggcgagac ggtgataccg tgcccggtca cgccgtacgt gttgcacagg tacacgagcg
     1321 ggccgcccca gccgcagccc acatcgagga tgtgcatgcc cggcttgagc tgcatgcact
     1381 cggccagctt gtcgagcttc ttctcctgcg cctgcgtgac gctgaagcca tcctcccaca
     1441 tcaggcacga gtacacgttc cattcgccgg cggtctggag gtagtagaac tccggatcct
     1501 gctcgtaatg ataggcggag cgagcggtgt cttcttcgac gccgtactcc tgatacatgc
     1561 gcgtgccgcc gggcggcaag gtctgcgcga tggtcgcttt ggtttcgtca gtcatcgatt
     1621 ccccccgtac gtgaaattag ccacggagac acagaggcac agagttttta ggacgcggac
     1681 gaacgcagat tacgctgatt ttgattcaaa ccatgttcat cggcacgctt cgcgtccgcg
     1741 tccaaagatt gggggtctct gtgttctcgg tgactctgtg gctttgattc cccccattaa
     1801 agagacgcga cgatgtggcg ggcgcgatct agatgccagg ccatctccag cctctcgaac
     1861 atcgcgatgg cttcgccgat cgccgcgcgc gcctgatcgg tcagcccccg gtcgcgatac
     1921 agcgtgccca ggtcgagcgt gctgacggcg acgagcggca gcgcgttgca ttcctgctgc
     1981 agcgcgatgc tggtcttgat cgcctgctcg gccttttccc agtcgggcgt cgccgcgcgc
     2041 gcgtagatgc gaccgaggat gcgctgcgct tcgccgagcg acatgcgctg ccgcgagcgc
     2101 tcggcggccg ccaccgccgc ttgggcgcgc gccagtgccg ccgtccagtc gccgtcgatc
     2161 acgtcgatct cggcgcgata cgcctgatac atcggcgtga acgccgacgc cttgttcgtc
     2221 tcggccaggc gcaggcagtc gtcgagatag cggcgcgccc gggcgaggtc gccttcttgc
     2281 aaatgcagat agcccatgca gccgtaggcg atgtacaacg gctggacgta ctgcttcggc
     2341 tcggcgatgc tcaggcagcg cttcaggtag gcggcggctt cgtcaaagaa cccgcagaag
     2401 cagtgataga agcccatcgt catcaggttg cccgcgatgc gactggggcg ctccagctct
     2461 tcggccagcg ccaggccgcg ctcggcgtgc ggcgtgcctt ccgcccgttc cccctggaac
     2521 cagaacgtcg aagcgtagaa agcgagtgag ccggcgagca gctcgacgtc gcggtatcgc
     2581 tcggcgaggg cgatgccctt cgccaacaag cccgccgctc tggcgaaatc gcccgacacg
     2641 gcgaccgcgc ggccgacgac gttgtacggc tgcatcagca atccttccag cccgagcttc
     2701 tcggccaggg ccatgcactt gccgaagctg tccagcgcct cgttcagccg ccccatcacg
     2761 tagtgaaacg cgccggcccc gccgtacacc tgcgccagtc gcacctcgtc gcccaacgat
     2821 tcggcggcgt tttgcgcgcg cttaagcgct tcgaggatgc tgtcgctcgg caggtacgcg
     2881 ccgacgcgcg ccagatcgac ggccagatcg acgacctgcc gctgcctctc cggcgtgacg
     2941 ggcaggcgcg acagcgtctc gagcgcctgc tcgaagagcg ccttggcttc ggcgttcgcg
     3001 ccgctgcgca tcgcctgatg ggcagccttc tccaggtagc cggctgcttt ctcgtcgtct
     3061 ccggccaggc tccagtgata ggccaggagt tgatcgtacc gggcgcggcc gcgctcgcgc
     3121 gcctgcgcac gctcgtacca ctcggcgacg gcgcgatgca gttgccgccg ctgcgcgaac
     3181 agcatcaggt tgtaggccac atcttgtgtg atgacgtgct tgaagatgta agccaggtcc
     3241 ggctcgggcg tatcgagcaa ggtcaggttc aatctttgca gcgcatcgag ataaccggcg
     3301 aggtatggcc ggtcagaatc gatcgggtag atgtcgtgca ggatgcggaa cgggaacatg
     3361 cgccctatga cgctcgccgc cttcaccgtc atctgctgag gcggcgtcag gcgatcgatg
     3421 cggctggtga tcacgccctg gatcgtgtcg gagaacgcga tcgcgcgcag gtcgccggcg
     3481 tccggcgcga cgcggcactc gccgccggcg atctcgatca agccggcgtc gcgcaaggcg
     3541 taggccactt cctcgctgaa gaagggatgg ccttctgctt tctcgcggat cagatcggcc
     3601 accggctcgg gcagtgatcg gacgtttagc cgctggcaca cgagcgcgag tgtatcctcc
     3661 gccggcagcg cttccaggcg caggcggcgc gtgtttggcg attgcatcaa ttgcagatac
     3721 tcgggcggcg gcggatcgct catcgggcgc gccgtgacga tgagcagcga tcgctctaag
     3781 cgccggctca cgagcagcgc gagcgcccac gaggcggagt cgagccagtg agcatcttcg
     3841 aggacgatca ctttcgggcg ccgatcgtcc ggcatccctt cggagcgagg caggtcattc
     3901 aaaaggcgca gcagcacgtc gcgcgcggcg tcgccgcgag cctgatccgt catgcttgca
     3961 gtcgtctcgt tctccggcaa gtccacgggc agcgccacgt tgagcagcgg cgccaggctt
     4021 gccacctgcg gatcgtgtcg cagtcgatct agaatctgct cgcgcctgtt ttcgggatcg
     4081 gtctcggcgt gcagaccgag cagcttcacg aagatgtctc gccaggcgtg atacggcgtg
     4141 ttcttctcga tcgcgtcgcc cgcgccgagc aggcaggcca ggcccagctc ctgcgcccgg
     4201 cgcagcgctt cgtcgacgag gcgcgacttg ccgatgccgg cttctccctc gagcactacc
     4261 gtcccgcccg gctcgccgtg cagcaggttc tgcatgcgct ccgacagcat cgcccattcg
     4321 gctgcccggc cgatcatcgt cacgtgcggg cgggcggtcg ccctggccgc gcggatcggg
     4381 cgatagatcg cgaccggctc ggccttgccc ttgactcgaa tcgccgggag ggattcgaac
     4441 tcgacctgcg cgtgcgcggc ctgggccgtc gccgcgtcgc ataagatgtc gtcgcgagcc
     4501 gactgcatca gccgcgccgc gagattgacc acgtcgccga ccatcgtgta ttcgcgccgc
     4561 gccgccgtgc cgaccgaccc acagaaggcc agcccgccgg ccacgccgat ggccgggcgc
     4621 agacccagct cgcgcagcag cgactggatg gcaagggcgg cctgtgcgcc gcgcgccgcg
     4681 tcgtcctcgt gcgtccaggg cggcagcccc agcgcggcga ccaggttcgc gcccgtgtcg
     4741 tccacgctaa tcttgtcgat gctcgcttcg tagcgataca gcacgctctg cagcgcgcgc
     4801 gtaatcatct gcgcctgttt gaggccgcgc ggcgccttat cgtcctgacc cggaaggttg
     4861 acgaagatca ccgttacccg gcgaagctcc gccagccatt ccgattggcc ggccgccagt
     4921 cgctcgagga tggcgccggg gatgtatgcg cgcagcggaa gctccatctc ggccttgagc
     4981 gccggccgca aagcccggcg cggcggcagc gaatcgcgtg cagactcgag ccgcatgctg
     5041 ccggtcggca gagcgtgacc tgtgaaccat tggcctgcca gcgaccacgc ctcgggcgac
     5101 acgatcacgt ctccgggcag agcttccgtc ttgatcgcac cgatttggga cagcggcgcg
     5161 cctgtcgcca caaactccca gcgcccgttc accccgccga cgtgtgccga ggtcatctcg
     5221 cccgccccga tgcccacgcg cagcgagagg cgcacgtcgc gcgccgccgc gtagtcgtgg
     5281 agcgccgcct gcgccgccag gccgcactgc gccgcgcggc gcacggcggc cgccagcgac
     5341 tcgtcgacag ccggccacag cgcgagcagc gcgtcgccgg cgaatttcac gacgtcgccg
     5401 ccgtgagcgt cgacccgctc gatcaactgc ccgaagtaat cgttcagcag gcgggagact
     5461 tcttcggcgc cggccggccc gagccgggcg agacgctcgg tgagcgcggt gaagccggtg
     5521 atgtcggcaa agaaaatcgc ggaggggaag cgctcggcgc cgggcgtgat gatcgggtca
     5581 ggatgttcgg cgtaccagcg cacgacgagc gatggcacgt aactggcgag cgtttcgagc
     5641 agcgcggaca aaccggagcc tcctcaaggc cgccgataga gtaacacaag tgatggctgc
     5701 cagtcaacct aacccaatgg taccggtgtg ctgctataaa cctggcaggt cttgtccgcc
     5761 ggtgttggcc gtgcgttgga cctcaagacc tgccaggtct acttcgacgg taccaggcgc
     5821 atatggctct ccgcctcgcg ctcgaccgcc tctcggctcc agggcatcgg ccgcgcctcg
     5881 ccggcgagcc acagggggaa ctggtcgaaa tagtgcgggc tgaccgggtg acccgacggc
     5941 ccgcccggca gcaccgcgac gcattgatcc cagtcgccca cgtcgtacac gacccta
//
LOCUS       DBNHXB010000079         4342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1859773, whole genome shotgun sequence.
ACCESSION   DBNHXB010000079 DBNHXB010000000
VERSION     DBNHXB010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1859773"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="AC162G_03065"
     CDS             <1..741
                     /locus_tag="AC162G_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5074828.1"
                     /translation="EIRFSIRMHDLEKGHRHVFDRIALAKAHIPHVMVEMPVLPGTLD
                     IMQDVLLELDRLNIDSINLLEFCFPWANAPAFNAKGYQIKQRPYRVLYNYWYAGGLPV
                     ARSELECLALLEFAIDRQLRIGVHYCSLENKHTGQIYQQNFGQPIPRTMYFSEKDYFL
                     KSAKVFGADIPLVVKRFRRMRYDEYVTNDDYGYVEFHLSKIKSLRELDIEIGLSSSVL
                     EVRDEEPYLRELKVDLAYPRVFDFATDV"
     gene            794..1501
                     /locus_tag="AC162G_03070"
     CDS             794..1501
                     /locus_tag="AC162G_03070"
                     /inference="COORDINATES: protein motif:HMM:NF014651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorD/DmsD family molecular chaperone"
                     /protein_id="HIR5074829.1"
                     /translation="MTRDASSIERDWVSILTGETLVLGLLSKTLYPYPEKTWLQTLAH
                     EDVFADIPLGEGQPDVQAGLTLLQTWSASCRSGLSDPAFDDLRADYTRLFIGPGKVIA
                     PPWESVYSNEERLTFQEQTLQVRAWYRQFGLEAERLHNEPDDHIGLELSFLAHLAQLA
                     LAALERQDRPAVDELLKAQRRFLSEHLLRWTPAWCDQVAQHARTDFFRGIALLTKGVL
                     KELSTILPVESLTEPAR"
     gene            1498..2709
                     /locus_tag="AC162G_03075"
     CDS             1498..2709
                     /locus_tag="AC162G_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5074830.1"
                     /translation="MNVLSALEQLAALDAAPIELDANRCVRARDRFSTCGACTRACPA
                     NALRVNGSVALDAEACVACRQCVHVCPTGVFDRNDNAAELLAFLPRLKDARIVELACA
                     RHPSPQTGPPETEAVVRIEGCLASLGPSVYLTLLASGIERIVVRLDACAECPIGAARP
                     MIEQTLNTAQVISNACRETNRITRLTEHPGKGWMRRPLYAKRTQVSRRDLFRRAIGEG
                     QQHVARKLALDRPGDQPDTPAEKSLPLERQRLLDALNRLLPVAAPANAEAPLEGLPFA
                     RLLVGEKCTACGVCARICPTGALQLIIGEDDSYQLTFLAGRCTGCGICLHLCEPGALR
                     RESPTLAELIATQPAALRSGTLRRCQKCGVKFAGESTASLCPVCDFRRRNPFGMRLPP
                     GFKPGAAQVSK"
     regulatory      2723..2883
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            2936..3565
                     /locus_tag="AC162G_03080"
     CDS             2936..3565
                     /locus_tag="AC162G_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HIR5074831.1"
                     /translation="MTWLDQLLDESAARHRHLCPRQVLGVRIGLAGAAALGLGAPRKD
                     KRLLVIAEADGCFADGIEVATNCTVGHRTLRIEDYGKIAATFVDVKTGNGVRVAPCLD
                     ARERACIYAPAEQRHYFAQLAAYQIMPDDELLAIQTVRLTTPVEAIVSRAGVRVDCAA
                     CGEEIINEREVEVGGTTLCRACAGQAYYRPVEEVLPLLVRLRETEAVGV"
     gene            3562..>4342
                     /locus_tag="AC162G_03085"
     CDS             3562..>4342
                     /locus_tag="AC162G_03085"
                     /inference="COORDINATES: protein motif:HMM:NF028293.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HIR5074832.1"
                     /translation="MKAGADVQASFRFTLAELSGALADLGVMLPLVLALITLNGVDAT
                     SAFVGIGLAYLVTALVYRLPIPVQPLKSVSAVALALGLSARLIVAAAWWNAALFLLMA
                     WTRADRLIARLFPKPVVRGIQLGLAVLLGRSAWNLIRAPDAAWPGDVIIAGATIPWVW
                     LIGAGTLTLLLLALRWRRDWAGLIAILFGIMLTVIHDGVPSLSFTLSLPTPASIVPRQ
                     DELWQALWLLAVPQIPLSLANSVFATADAARQYFGAQAERVS"
BASE COUNT          781 a         1332 c         1389 g          840 t
ORIGIN      
        1 gagatccgct tcagcatccg gatgcacgat ctggagaagg gccaccgtca cgtgttcgat
       61 cggatcgcac tggcgaaggc ccacatcccg cacgtcatgg tcgagatgcc cgtgctgccc
      121 ggcacgctgg acatcatgca ggacgtgctg ctggaactcg atcgtctgaa catcgacagc
      181 atcaacttgc tggagttctg tttcccatgg gcgaacgcgc cggccttcaa cgccaaaggc
      241 taccagatca aacagcgccc gtaccgcgtg ctgtacaact actggtacgc cggcgggctg
      301 ccggtcgctc gcagcgaact ggagtgcctg gcgctgttgg agttcgccat cgaccggcaa
      361 ctccgtatcg gcgtgcacta ttgctcgcta gaaaacaagc ataccgggca gatttatcaa
      421 cagaacttcg gccaaccgat ccccaggacc atgtacttct cagaaaagga ttacttcctg
      481 aaatcggcca aagtcttcgg cgcagatatt ccgctggtgg tgaagcgatt caggcggatg
      541 cgctacgacg agtacgtgac gaacgacgac tacggctacg tcgagtttca cctgagcaaa
      601 atcaagtcgc tgcgagaact cgatatcgag atcggcttgt cgtcgagcgt cctcgaagtg
      661 cgcgacgagg agccgtatct acgggagttg aaagtcgatc tggcctatcc gagagtgttc
      721 gactttgcga cagatgtgta aggcgtcccg aaccggccac aggcgcgtcg aatcaccata
      781 aaggacgaaa ataatgacac gcgatgcatc ttccatagag cgggactggg tttcaatctt
      841 gaccggggag actttggtcc tggggctgtt gagcaagaca ctgtaccctt atcccgagaa
      901 gacgtggctt cagacgctgg cccatgaaga tgtcttcgca gatatcccgc tcggcgaagg
      961 acagcctgac gtgcaggccg ggctgaccct actccaaaca tggagcgcca gttgccggag
     1021 cggcctgtct gacccggcat tcgacgacct gcgcgcggac tatacccgcc tgttcattgg
     1081 ccccggcaag gtcatcgcgc cgccctggga atcggtttac tctaatgaag agcgactgac
     1141 gtttcaagag cagacgctgc aggtgcgcgc atggtatcgg cagtttggcc tggaggccga
     1201 aagactgcac aacgaaccag atgaccacat cgggctggaa ctatccttcc tggcacatct
     1261 ggctcaactc gccctggccg cgcttgaacg gcaagatcgg ccggccgttg atgaactgct
     1321 gaaagcgcag cgccgtttct tgtccgagca tctgctgcgc tggacgccgg cctggtgcga
     1381 tcaggtcgcg caacacgcgc gcaccgattt cttccgcggc atcgcgctct tgacgaaggg
     1441 tgtgctcaag gaattgtcga ctatccttcc ggttgagtcc ctgacggagc ctgcccggtg
     1501 aacgtgctgt ccgcgctcga gcaactggcc gcgctcgatg ccgcgccgat cgaactcgac
     1561 gcgaatcgct gcgtgcgcgc tcgcgatcga ttctcaacgt gcggggcatg cacgcgtgcg
     1621 tgcccggcga atgcactgcg tgtgaacggc tcggttgcac tcgacgccga ggcgtgcgtc
     1681 gcctgcaggc agtgcgtcca cgtctgcccg acgggcgttt tcgatcgcaa cgacaacgcc
     1741 gcagaactgc tggcctttct gcctcggctg aaggatgcgc gtatcgtcga actggcctgc
     1801 gcgcggcatc cctcgccgca gaccggcccg cccgaaacgg aggctgtggt tcgcatcgag
     1861 ggctgcctgg cctcgcttgg accgtcggtg tatctgacgc tgctcgcatc gggcatcgag
     1921 cggatcgtcg tgcggcttga cgcctgcgcc gagtgcccga tcggcgccgc ccgaccgatg
     1981 atcgagcaaa cgttgaacac agcccaagtg atttcgaatg cgtgccgtga aaccaaccgc
     2041 atcactcgac tgaccgaaca ccccggcaaa ggctggatgc gccgtccgct gtatgcgaaa
     2101 cgaacgcagg tgtcccggcg cgatctgttc cgcagggcca tcggcgaagg ccagcagcac
     2161 gtcgctcgca agttggcgct cgatcgaccg ggggaccaac ccgacacgcc tgccgagaaa
     2221 agcctgccgc ttgaacggca gcggctgctc gatgccttga acagactctt gcccgttgct
     2281 gcaccagcaa acgccgaagc accacttgag gggctaccat ttgcgcgctt gctggtaggc
     2341 gaaaaatgta cagcctgtgg cgtgtgcgcc cgcatctgcc cgaccggtgc gctacaactg
     2401 atcatcggcg aagatgactc gtatcaactg acgtttctgg ccggccggtg caccggttgc
     2461 ggcatctgtc tacacctgtg cgagccgggc gcattgcgcc gcgaaagccc gacgctcgct
     2521 gaactcatcg ccacacaacc ggccgctctc cggtcaggta ccctgcgccg atgccagaag
     2581 tgcggcgtga agtttgcggg tgagtcgact gcatcgttgt gtcctgtgtg tgacttccgg
     2641 cggcgcaacc cattcggcat gcgcttaccg cccggcttca agccgggagc cgcacaagtc
     2701 tcgaaatgac gtaggaacct gccaggttca gggtctccga gccctctcgc ctaatgaccc
     2761 ggtctacggt gagagggccg tgcctctcgt gatgggaggg agacgcatca cctgcgtctc
     2821 gtgggccacg ctggaaacgg cgcgacctcc tcgtgctcgg aaaggagacg atccaatcgg
     2881 tctgatgact gagggccgtc tcgcttgaga cggccgtttt gatcggaggt tcgttatgac
     2941 atggctcgat cagcttttgg acgagtcggc cgcgcggcac cgtcatctgt gcccgcgcca
     3001 ggtattgggt gtgcggattg ggttggccgg cgcggcagcg ctggggctgg gtgcgccgcg
     3061 caaggacaag cggctgctgg tcatcgcgga ggccgacggc tgcttcgcgg acgggatcga
     3121 agtggcgacg aattgcaccg tcggccaccg cacactgcgc atcgaggact atggcaagat
     3181 cgcggcgacg ttcgtcgacg tgaaaaccgg caatggagtg cgcgtcgctc catgtctcga
     3241 cgcacgtgaa cgcgcctgca tctatgctcc tgcagaacag cggcactact tcgctcaact
     3301 ggcagcctat cagatcatgc cggatgatga gttgctcgcc attcaaacgg tacgcctgac
     3361 gacgccagta gaagctatcg tcagccgtgc cggtgtgcgc gtcgattgtg ccgcctgcgg
     3421 cgaggagatc atcaacgagc gcgaggttga agtcggcggc acgacgctgt gccgcgcctg
     3481 cgccggacaa gcctattacc ggcccgtgga ggaagtattg cctctacttg tgcggctaag
     3541 ggagacagag gcggtcggcg tatgaaggcc ggggcggacg tgcaagctag cttccgtttc
     3601 acgctggctg aactgtccgg cgcgctggcg gacctggggg tgatgctgcc gctggtattg
     3661 gcgctgatca cgctcaacgg cgtcgatgcc accagcgcgt tcgtcgggat cgggctcgcc
     3721 tacctggtga cggctctggt ttaccgcttg ccgattccgg tgcagccgct caaatcggtt
     3781 tcggcggtag cgctggcgct cggactgtcc gcgcggctca tcgtcgcggc cgcatggtgg
     3841 aacgcggcgc tattcctgct gatggcgtgg acgcgtgccg accggctgat cgcgcgactg
     3901 tttcccaagc cggtcgtgcg cggcattcag ttggggttgg ccgtgctgtt gggccgctcg
     3961 gcgtggaacc tgatccgtgc gcccgatgcg gcgtggccgg gcgacgtgat cattgccgga
     4021 gcaacgatcc cctgggtgtg gttgatcgga gcgggcacac tcacgcttct gctgcttgcg
     4081 ctgcgttggc ggcgcgactg ggccggactg atcgcgatcc tgttcggcat catgctgaca
     4141 gttatccacg atggcgtgcc gtcgttgtcg ttcaccttgt cgctgccaac accggcatct
     4201 atcgttcctc gccaggatga attgtggcag gcgctgtggc tgctcgcggt gccgcaaatt
     4261 ccactaagcc tggcgaactc ggtcttcgcc acggcggacg cggcgcggca gtacttcggg
     4321 gcgcaggctg agcgtgtttc gc
//
LOCUS       DBNHXB010000080        10015 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1524384, whole genome shotgun sequence.
ACCESSION   DBNHXB010000080 DBNHXB010000000
VERSION     DBNHXB010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1524384"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="AC162G_03090"
     CDS             complement(<1..405)
                     /locus_tag="AC162G_03090"
                     /inference="COORDINATES: protein motif:HMM:NF018213.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HIR5074833.1"
                     /translation="MTNPAILWRRLDQPGHESARLSLQKTGWQLAGTAVFAHQQQPCR
                     LDYLVVCDSQWQTVSGRVAGWVGDERVEIELSVDSARRWWVNGTECPVVAGCADLDLN
                     FSPSTNLLPIRRLNLAVGQEAEVTAAWLRFPGF"
     gene            complement(448..1155)
                     /locus_tag="AC162G_03095"
     CDS             complement(448..1155)
                     /locus_tag="AC162G_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074834.1"
                     /translation="MTQRSILAGRTPNVVIRAGGDVRVEGRDSDRVLADTESRWGLKV
                     ERRSESEIGRARAKVGDRVLFDVRLNVRNPLKKESLGEVIEVQIGGSGQVHVPRSSNV
                     KVYTGKNAEVQDIQGSVTVYAGLDARVRGVHTLVLVSAGGSLDFECEQVAGDDVKFTA
                     GRDLRCHIRNLTDARVMVDDLGGYWEGIIGEGRTKIRLKAGGDVTLVTDQVITGQPPY
                     DVIGKVERPNAASSENG"
     gene            complement(1213..1875)
                     /locus_tag="AC162G_03100"
     CDS             complement(1213..1875)
                     /locus_tag="AC162G_03100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5074835.1"
                     /translation="MSLRASKAYKGIAMEGIIARWYAKTARRDDEFKSLVSRVSEILT
                     AGSRVLEVAPGPGYLSIDLAKLGKYQVTGLDISKSFVEIARTNARQAGVQVDFRHGNA
                     ADMPFADETFDFIICTAAFKNFTQPVQALAEMYRVLRPNGEALISDLRRGAAQADINQ
                     TVNRMGLNRVNDFITKWTFRHFLLKNAYTPEEIRRFAAQTHFRQCEIREDAIGMEIWL
                     EK"
     gene            complement(1957..3210)
                     /locus_tag="AC162G_03105"
     CDS             complement(1957..3210)
                     /locus_tag="AC162G_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5074836.1"
                     /translation="MKSSYETVASVSNQKTNRRALGVIFFIMLMDIIGLGILAPVAPY
                     IVARYSHDALMVTLLTTIYAAAQFTAAPMLGKISDRIGRRPVLLVCVFGSAIGYFIFG
                     IGGALWVLFLARLIDGITGGNISTAAAYIVDVSRPEERAKNFTLMGMAFGLGFILGPA
                     LGGALGQLSVDAPMFAAGVISLVSVGVIYFLLPESLPKERRETAPLRLSDFNPFASIG
                     YMLRKPGLGVLLVVYALFNFAFNGANSTAGVFLVDKFAAQPWQIGLLFVMVGIATAVV
                     QAALIGRLVPMYGEKRMAMVSLIGNAVGGLLIFAAPALWLVYPISLVQAAITGFIWST
                     MGTLTANRVSEREQGLLAGVNTAVAGLMAALGPLWAGAMYDTVMPGAPYWMGAIILGV
                     ACLVLARVKVSVRASQPVSVQVAES"
     gene            complement(3223..4119)
                     /locus_tag="AC162G_03110"
     CDS             complement(3223..4119)
                     /locus_tag="AC162G_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5074837.1"
                     /translation="MAVNTADSSTVAAPEHYTTGCVASKDGTTIGYRQLGHGPGLVLL
                     HGMMSSGQNHMQLAEALADAFTVYVPDRRGRGLSGPYSEDYSIQKDVEDMDALLTKTG
                     AHNVFGVSAGGIISLQAALTLSAIHKAAIYEPPLFINDSVPTAVMTRFDKEMAQGKVA
                     AALVTAMKGSQLGPPIFNAMPRWLAERLTTMAMAQDDKNVKGGYVPMRALAPLLHYDF
                     QLSVEKSGKLDSFRAIRAEVLLLGGSKSPAYFRDALDALEKVLPRVKRVELPGLDHAA
                     SWNTDRGGQPEPVAQELRQFFA"
     gene            complement(4264..5106)
                     /locus_tag="AC162G_03115"
     CDS             complement(4264..5106)
                     /locus_tag="AC162G_03115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5074838.1"
                     /translation="MKIALVTPYDYPYPGGVTEHIRHLDREFRARGHETRILAASSAA
                     DENLEPHIIKVSGDVTPIPFNGSVARITLSPEVFERVGQILNEERFDVVHVHEPGVPL
                     LSLAVLLHAPRHAINVGTFHAYQETNPMYEYARPLVEYLFSQLVGIVLLEAMAAGVPV
                     VASDIAGYRSVLSQGEEGRLVKPKDEQAIAEAAIALLRDPRQRAEMGARGRTTAARYD
                     WRVVAGRVLDYYGELRQARVSQPAQPSFRHHIAKQLALLTGGVSRILSPASGAITHPI
                     TRLG"
     gene            complement(5246..7954)
                     /locus_tag="AC162G_03120"
     CDS             complement(5246..7954)
                     /locus_tag="AC162G_03120"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5074839.1"
                     /translation="MSEHQRPLHQESELLSTKLTPPRLRASIVSRERLLARLNEGLER
                     KLTLLSAPPGSGKTTLASEWLAARTEPVAWVSLDKGDDDPIRFWRYVLTACRAFDPAL
                     GRSALVALRASQQPVFEPVLTPFINEMAQLPHRCILVLDDYHVITLRQVHDTLTFLLD
                     HFPATLHLVLMTRSEPPLPLARLRARDELSEFNAADLRFSLDEIRAFFQQTLRLPLST
                     EAIARLEARTEGWAAGLRLVALALEGRQKPTETEQFLATLAGEHRHILEYLTGEVLAN
                     QPESLQVFLLQTSVLSRLTASLCDAVTDRDDSASVLERLVRANLFLIPLGGEGDQTWY
                     RYHTLFAEAMLHVARQRFGESGLQTLYDKAARWHAAQGFLAEAIEASIDARDFGRAAT
                     LVEQAVNVRSFSEPQTLRRWAEKLPQEMLHDHPILCLNYAMALLFTSDRYAPATATLA
                     ETPLQAAEAAWRKTEDEHRLGQVFAFRSMVAMWQGDWIRSFAWAKEALALLPEHEVYW
                     RGISLLIASIQDMLAGKIDSVQRMALEARALCEAAQNIHGVSGVTLLLAGTFAAQTEL
                     DQAEALYRQVLDEAGDAEELLDDRGFAQLGLGTIAFERNDLAPAEQHAAQALEIAQRR
                     PEQDVQVHASLLMARIQHARGQMAQAQETLRVLAARTHQAVWLREVQAWQARFSLDAG
                     DLVAAQRWQATFAQHTDGVPKTQQEQEALILARMLIAEGESEAALKLLERWRRDAHAH
                     ARTRSELEIRCLQALAHAAQMDTQRALKTLVRALTLAQPSGLRRVFLDEGQPMALLLQ
                     AVIPDLKKRPLATYATLLLKAFASTRSIQMLSAPTVSSLLLEPLSPQEQRVLRLLAAG
                     LSNPEIARELVVSTNTIKTQIKSIYRKLNVNTREEAREAARQLKLL"
     gene            complement(7951..9081)
                     /locus_tag="AC162G_03125"
     CDS             complement(7951..9081)
                     /locus_tag="AC162G_03125"
                     /inference="COORDINATES: protein motif:HMM:NF017984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HIR5074840.1"
                     /translation="MARSLSDAQVRRLHLSAQRLHPRQAQSVSSAAQVLKDICGVQAQ
                     DAQAAILAVRVRSAGLTASGVERARLQERSVVRTWCMRGTLHLLAAEDVGWLLSLFGP
                     IFANANRRRRAQLGLDDDASAKGVRVIRDALAKQGPLTRAEIAKALARRGIPAEGQAT
                     IHLIYLAALQGIVCQGPDRGRQPTHILLKDWIDLEPALPANAARAELARRYLLAYGPA
                     GPDDLAHWSGLPIGEMRAAWQSIAGELIEVNAAGRPAWMHKRHAAWLGRPAARSPIVR
                     LLPSFDTYLLGYRDRSLALSPEHAKRIHPGGGIIHPALTADGRVLGTWRSQRRRDGIE
                     VIVEPFEALALTVLRGIETEVADLARFLDRKATLSVLSKISE"
     gene            complement(9091..9348)
                     /locus_tag="AC162G_03130"
     CDS             complement(9091..9348)
                     /locus_tag="AC162G_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5074841.1"
                     /translation="MQDIAERATVNRVTFYARFQDKYALLEYTVREMVRQSLHSQVPE
                     GSPYPVEYHCAERPLPTSRKCSMIVILKWMKWSIHNNRPIC"
     gene            9397..9528
                     /locus_tag="AC162G_03135"
     CDS             9397..9528
                     /locus_tag="AC162G_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074842.1"
                     /translation="MSKSPFAGGQGIGWEFEFILAAVALALLFLGAGSIALDPVVGL"
     gene            9776..>10015
                     /locus_tag="AC162G_03140"
     CDS             9776..>10015
                     /locus_tag="AC162G_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074843.1"
                     /translation="MSNQDIQATWQYHDGTKHPAGRLMNPQHHFDPSRQPLLFKIYKD
                     LPSISLPLDPSPSVPALEAIAGEIPAAGGESAPDLP"
BASE COUNT         1914 a         3132 c         3059 g         1910 t
ORIGIN      
        1 gaaacctggg aaacgcaacc aggccgcagt cacttcagcc tcttgcccga cggccagatt
       61 cagacggcgg atcggcaaca gattggtcga ggggctgaag ttcaaatcta gatcggcaca
      121 gcccgccacc accggacact cggttccgtt gacccaccag cggcgagccg agtccaccga
      181 aagctcgatc tcgactctct cgtctccaac ccaaccggcg acccggccag agaccgtttg
      241 ccactgtgag tcgcatacga ccagatagtc cagccggcag ggctgctgtt ggtgggcaaa
      301 cacggctgtt ccagcaagct gccagccggt cttctgcagg gacagtcgcg ctgattcgtg
      361 cccgggttgg tccagtctgc gccagaggat tgctgggttc gtcacacttt cctcctcaaa
      421 atggtgtggc gctgagcctc ctctcgctca gccgttctcg ctgctcgccg cgttcgggcg
      481 ttcgaccttg ccgatgacgt cgtacggggg ttggcccgta ataacttgat ctgtcaccag
      541 cgtcacgtca ccgccagcct tgagccgaat cttggtgcgc ccttcgccaa tgatcccctc
      601 ccagtaaccg ccgaggtcgt caaccatcac tctggcgtca gtcaggttgc ggatgtgaca
      661 gcgcaaatcg cggccggctg tgaatttcac gtcatcaccg gccacttgtt cacactcaaa
      721 gtcgagcgac cctccggcag acacaaggac aagcgtgtga acaccgcgaa cgcgcgcatc
      781 gaggccagcg taaacggtga cactgccctg aatgtcctgc acctcggcgt tcttgccggt
      841 atacaccttc acgttgctgc tccgagggac atgcacttgg ccgctaccgc cgatctggac
      901 ttcgatgact tcgccaagcg actctttctt caagggattc ctcacgttca agcgcacgtc
      961 gaacaacacg cgatcgccaa ccttcgcgcg ggcgcggcca atctccgatt cgctgcgccg
     1021 ttcaactttc aacccccagc gactttcggt gtctgccagg acgcggtcgc tgtcccggcc
     1081 ttcaacgcgg acatcaccac cggcccggat gacgacgttg ggagtccgtc cggcgagaat
     1141 cgatcgttga gtcatgatgt ttgagctcct gtctctatct taccacgagt catcattgac
     1201 tggccgatgc ggtcacttct ccagccagat ctccatgccg atggcgtcct cgcggatttc
     1261 gcactgtcta aagtgcgtct gcgcggcgaa ccggcggatt tcctctggtg tgtacgcatt
     1321 cttgagcaga aagtgcctga atgtccattt ggtgatgaag tcattcacgc ggttcaagcc
     1381 catgcggttt acggtctggt tgatgtcggc ttgcgcagcg ccccgccgca aatcggagat
     1441 gagcgcctcg ccgttgggtc tcaagacgcg atacatctcg gcgagcgcct gtaccggttg
     1501 ggtgaagttc ttgaacgccg ccgtgcagat aataaaatcg aaggtctcat cggcaaaggg
     1561 catgtcggcc gcgttaccat gccgaaagtc aacctgcact cctgcctgtc tggcattcgt
     1621 tcgggcgatt tcgacaaagg acttgctgat gtccaggccg gtcacttgat acttgcccag
     1681 tttagccaga tcgattgaca ggtaacctgg accgggagcg acttcgagca cacgactgcc
     1741 cgctgtgagt atctcgctca ctcggctgac caacgactta aactcgtcgt ctcgccgggc
     1801 agtcttggca taccacctgg caatgatgcc ttccattgcg atacctttgt aggcctttga
     1861 agctctgagt gacataggac tattcctcct gttttgtcgg ccaggcttcc gaagcctgcg
     1921 ctaaaccgag gcttcggaag cctttatctg gctacactac gattcagcta cttgaacact
     1981 gactggctga ctcgctcgca ctgacacttt aacgcgtgcg agcacgagac aagcaacacc
     2041 cagaatgatc gcgcccatcc agtacggcgc gccaggcatc accgtatcgt acatagcccc
     2101 ggcccacagc gggccgagcg cggccatcag gccggctaca gcggtattca cgccagccag
     2161 caggccctgt tcgcgctccg acacacggtt ggccgtgagc gtgcccatcg tcgaccagat
     2221 aaagccggtg atggcggcct gcacaaggct gatcggatac accagccaga gcgccggcgc
     2281 ggcgaagatg agcagcccgc ccacggcgtt gccgatcagg ctgaccatcg ccatgcgctt
     2341 ctcgccgtac atgggcacga gccggccgat gagcgcggcc tgcacgacgg cggtcgcaat
     2401 accgaccatg acgaagagca ggccgatctg ccagggctgc gcggcgaact tgtccaccag
     2461 gaacacaccg gccgtgctgt tagcgccgtt gaaggcgaag ttgaacagcg cgtacaccac
     2521 cagcagcaca cccagtcccg gcttgcgcaa catgtagccg atcgacgcga acggattgaa
     2581 gtcgctcagg cgcagcggcg cggtctcgcg ccgctctttc ggcagcgatt cgggcagcag
     2641 gaagtagatc acgcccacgc taaccagcga gatcacgccg gcggcgaaca tcggcgcgtc
     2701 gacgctgagc tggccgagcg cgccgcccag cgccggcccc aggatgaagc ccaggccgaa
     2761 ggccatgccc atcagcgtga agttcttcgc gcgctcttca ggcctggaga catcgacgat
     2821 gtaagcagca gccgtggaga tgttgccgcc ggtgataccg tcgatcagcc gggccaggaa
     2881 cagcacccac agcgcgccgc cgatgccgaa gatgaagtag ccgatcgccg agccgaagac
     2941 gcacacgagc agcacaggcc ggcggccaat ccgatcgctg atcttgccca gcatgggcgc
     3001 ggcggtgaac tgcgcggcgg cgtaaatcgt cgtcagcagg gtgaccatca gcgcatcatg
     3061 gctgtagcga gccacgatat aaggcgcgac cggcgccagg atgccgaggc cgatgatgtc
     3121 catcagcatg atgaagaaga tcacgccgag ggcacgccgg ttcgttttct gatttgatac
     3181 ggatgcaacc gtctcgtaac tggatttcat gtcagacctc ccttaggcaa aaaattggcg
     3241 cagttcttga gccaccggtt ccggctggcc gcctctatcg gtattccatg aagccgcatg
     3301 atcgagtccg gggagctcga cacgtttgac acgtggcaag accttctcca gggcatcgag
     3361 ggcatctcta aagtaagccg ggctcttgct cccgccgagc aaaagaacct cggcgcgtat
     3421 agccctgaag ctgtccagct tcccactctt ctcgacgctg agctgaaagt catagtgcaa
     3481 gagtggagcg agcgccctca tcggtacgta gccgcccttg acgttcttat cgtcttgtgc
     3541 catcgccatc gtcgtgagcc gctcggccag ccagcggggc atggcattga agatgggcgg
     3601 ccccaactgc gatcctttca tggccgtgac gagcgcagcg gctaccttac cctgggccat
     3661 ctccttgtca aagcgcgtca taacagcagt cggcaccgag tcgttgatga acagcggtgg
     3721 ttcgtagatg gcggctttgt gaatagcgga cagggtgagc gctgcttgga gcgagatgat
     3781 gcctcctgcg ctgaccccga agacgttgtg agcgcccgtt ttggtgagga gagcgtccat
     3841 atcttcaacg tccttctgta tgctgtaatc ttcgctgtac gggccgctta ggccacgacc
     3901 acggcggtcg ggcacgtaga cggtgaaggc atcagccagc gcctcggcga gctgcatgtg
     3961 gttttggcct gacgacatca tgccatgcag cagcaccagg cccgggccgt ggccgagctg
     4021 gcggtagccg atggtggtgc catctttgga ggctacgcag ccggttgtat aatgctccgg
     4081 cgcggctact gtcgatgaat ctgctgtatt aactgccatc ttgaattctc cttttcctac
     4141 tcatcttcgc gtgcgatatc gggttgaacc acgtcagaat cgtttcaaga accttatcca
     4201 tctgtccgtc tcctttgttt ggtttgatgc gcggattgta gaatcgacgc gggtgaacga
     4261 gcatcacccc aggcgggtga ttgggtgggt gattgcgccg ctggccggtg acaggattct
     4321 gcttacgccc cctgtcagga gcgcaagttg ctttgcgatg tggtgtcgga acgacggctg
     4381 cgcgggctga gacacacgcg cttgcctcaa ttccccgtag tagtccagca cgcgccccgc
     4441 gacgacgcgc caatcgtacc gggcggcggt ggttcgcccg cgcgccccca tctccgcccg
     4501 ctgtcgcgga tcgcgcagca acgcaatcgc ggcttcggca atcgcctgct catctttggg
     4561 cttcaccagc cggccctctt caccctggct gagcacgctg cgatagccgg cgatgtctga
     4621 cgcgaccaca ggcacgccgg ccgccattgc ttccagcagc acgattccga ccaactggct
     4681 gaagaggtat tccaccagcg gtcgtgcgta ttcgtacata gggttcgtct cttgatatgc
     4741 gtgaaatgtg ccgacgttaa tcgcgtgtcg cggagcgtgg agcagcaccg ccaggctcag
     4801 caggggcacg ccaggttcgt gcacgtggac cacgtcgaag cgctcttcgt tcagaatctg
     4861 cccgacgcgc tcgaagacct cgggggacag cgtgatgcgc gcgaccgagc cgttgaacgg
     4921 gatgggcgtc acgtcgccgc tcaccttgat gatgtgtggc tccagattct catccgccgc
     4981 actggaggcg gcgaggatgc gtgtctcgtg gccgcgagcc cgaaactcgc ggtcgagatg
     5041 tcgaatgtgt tcggtgacac cacccggata cgggtaatcg tagggcgtga ccaatgcgat
     5101 cttcataact cacctcacgc catgtgatga tttaagcgca acagcaccga ctgtagcgcc
     5161 tcatggggtg agcgtgcatc gcccagggtg ggtgatcagg aggatgatta gggagagtga
     5221 tataggtggg acgactgagg acggtttaca gcagcttcag ctgacgagca gcctcacgag
     5281 cttcctcacg cgtgttgacg ttgagcttgc ggtagatgct cttgatctgc gtcttgatcg
     5341 tgttggtgga cacgacgagc tcgcgcgcga tctcaggatt ggagagaccg gcggcgagca
     5401 ggcgcagcac gcgctgctct tgtgggctaa ggggttcgag caagagtgat gacaccgtcg
     5461 gcgcggagag catctgaatc gatcgggtcg aagcgaaagc cttcagcagg agcgtcgcat
     5521 aggtggcaag gggacgtttt ttcagatcgg gaataacggc ctgcagcagc aaggccatgg
     5581 gctggccttc atccagaaag acacgtcgca agccgctggg ttgcgccagc gtcaatgccc
     5641 gcaccagtgt cttgagggcg cgctgcgtgt ccatctgggc agcgtgcgcc agtgcttgca
     5701 ggcaccggat ttccagctcg ctgcgcgtcc gcgcgtgagc gtgtgcgtcg cgccgccaac
     5761 gctcgagcaa cttcaaggcc gcctcggatt ccccttcggc gatgagcatt cgggcgagga
     5821 tcagggcttc ttgctcttgt tgtgtcttcg gcacaccgtc agtgtgttgg gcgaaggttg
     5881 cctgccagcg ctgcgcggcg accagatcac ccgcgtcgag cgagaaacgc gcctgccacg
     5941 cctgcacctc acgcagccac accgcttgat gcgtccgggc agcgagaaca cgcagcgttt
     6001 cctgcgcctg cgccatctgc ccgcgcgcgt gctggatgcg cgccatcagg agcgacgcgt
     6061 gcacttgcac atcctgctcg ggtcgccgtt gcgcgatctc cagcgcctga gccgcatgtt
     6121 gttcggccgg cgccagatca ttgcgttcaa aggcaatcgt gccaaggccg agctgggcaa
     6181 agccgcgatc gtccaggagt tcctcggcgt cgccggcttc atcgagcacc tgccgataga
     6241 gcgcctcagc ctgatcgagt tccgtttgcg cggcgaacgt tcctgctagc aggagcgtca
     6301 cccccgatac gccgtggata ttctgagccg cctcgcacaa ggctcgtgct tccagggcca
     6361 tgcgttgcac cgaatcgatc tttcccgcca acatgtcttg gatggaagca atgagcaggc
     6421 tgatgccgcg ccagtacacc tcatgttcgg gcaataacgc cagcgcttct ttcgcccagg
     6481 cgaacgatcg aatccaatcg ccctgccaca tggcaaccat cgagcggaaa gcaaagacct
     6541 ggcccagcct gtgctcatcc tcagtttttc gccaggcggc ttcggccgct tgcagcggtg
     6601 tctccgccaa cgtggccgtc gccggcgcat atcgatcgga ggtgaataac aacgccatcg
     6661 catagttgag acataagatc gggtgatcgt gaagcatctc ttgcggcagt ttctcggccc
     6721 agcggcgcag cgtctgaggc tcggagaaac tgcgcacatt cacggcctgt tcgacgagcg
     6781 tcgccgcgcg cccgaagtcg cgcgcgtcga tcgaggcttc gatcgcttcg gcgagaaagc
     6841 cctgcgcggc gtgccaccgt gcggctttgt cgtacagcgt ctgcaggcca ctctcaccga
     6901 aacgctgccg cgccacgtgc agcattgctt cagcaaacag cgtgtgatag cgataccacg
     6961 tctggtcgcc ctcgccgcca agcgggatga ggaacaggtt ggcgcgcacc agtcgttcaa
     7021 gaaccgaagc gctatcgtcg cgatcggtca ccgcatcgca cagcgaagcg gtcagtcgac
     7081 tgagcacgct ggtctgaagc agaaagacct ggagagactc gggttgattg gcaagcacct
     7141 cgccggtgag atactcgagg atgtgccgat gctcgccggc gagcgtcgca aggaactgct
     7201 cggtctccgt tggcttctgt cgcccctcca gcgccagcgc gaccagccgc aaccccgccg
     7261 cccaaccctc ggtccgcgct tcgaggcgcg cgatcgcttc ggtcgagagc ggtaggcgca
     7321 atgtctgctg aaagaaagcc cggatctcgt cgagcgagaa acgcaagtcg gcagcgttga
     7381 attcgcttag ctcgtcgcga gcccgcaggc gggccagcgg cagcggcggc tcgctgcggg
     7441 tcatcagcac gagatgcagt gttgccggaa aatgatcgag cagaaaggtt aacgtatcat
     7501 gaacctggcg caaagtaatg acgtggtaat catccagcac gaggatgcag cgatgaggca
     7561 actgcgccat ctcattgatg aatggcgtga gcaccggctc gaacacgggc tgttgtgacg
     7621 cgcgcagcgc caccaaagcc gatcggccca gcgcaggatc aaacgcgcga caggcggtga
     7681 ggacgtagcg ccagaagcgg atcggatcat cgtcgccttt gtccagcgac acccacgcca
     7741 ccggctcagt gcgggcggcg agccattcgc tcgccagcgt ggttttgccg gagcctggcg
     7801 gagcggacag cagggtcagc ttgcgctcga gtccctcatt cagccgggcc agcaaccgct
     7861 cgcgcgacac gatggacgcg cgcagacgag gaggcgtcag tttggtgctc aggagttcac
     7921 tttcctggtg cagcgggcgc tgatgctcac tcattccgat attttactca atacgctcaa
     7981 cgtcgctttc ctatccagga agcgcgccag gtcggctacc tcggtttcaa tcccacgaag
     8041 gacggttagc gcaagcgcct cgaacggctc cacgatgact tctatgccgt cccgacgccg
     8101 ctggctgcgc cacgtcccca gcacgcggcc atccgccgtc agtgccggat ggatgatccc
     8161 gcctccggga tgaatgcgct ttgcgtgttc gggcgataag gccaggctgc gatcacggta
     8221 tccgagcagg taagtgtcga agctcggcag caggcgcacg atgggagaac gcgccgccgg
     8281 ccggcccagc cacgcggcgt gccgtttatg catccacgcc ggccggccgg cagcgttcac
     8341 ttcgatcaat tcgccggcaa tggactgcca ggcggcacgc atctcgccga tcgggagacc
     8401 cgaccagtgc gccaggtcgt ccggccccgc cgggccgtaa gcgaggagat agcggcgcgc
     8461 aagttccgcc cgcgccgcat tcgccggcag cgctggttct aaatcgatcc agtctttgag
     8521 cagaatgtgg gtcggctgcc tgccgcgatc aggcccctgg cagacgatgc cttgcagagc
     8581 ggccaggtag atgaggtgaa tagttgcctg gccttcagcc ggaatgccgc gccgggccag
     8641 cgccttcgca atttcggccc gagttaacgg cccttgttta gcaagcgcat cgcggatgac
     8701 gcggacgcct ttcgcagaag cgtcgtcgtc cagacccaat tgcgctcgtc gccgccgatt
     8761 ggcgttggca aaaatcgggc cgaacagtga caggagccag ccgacgtctt ccgctgccag
     8821 cagatgcagc gtgccacgca tgcaccacgt ccgcacgacg gaacgttcct gcagccgcgc
     8881 gcgctcgacg ccggaggcag tcagcccggc gctgcggacg cgcaccgcga ggatggcggc
     8941 ttgcgcatcc tgtgcctgca cgccgcaaat atccttcaga acttgcgccg cgctcgaaac
     9001 cgattgggct tgccgtggat gcagccgctg cgcgctgagg tgaagccggc ggacttgagc
     9061 gtcggacaga gaacgcgcca tggacccgga tcagcagatt ggcctgttat tgtgaattga
     9121 ccacttcatc cacttcagga taacaatcat actacatttc cttgaggtag gaagtggtcg
     9181 ctccgcgcag tgatattcga ccggatacgg tgagccttcc ggcacctggc tgtgcaagct
     9241 ctgcctaacc atctcgcgca cagtgtactc cagcagcgca tacttatctt ggaagcgggc
     9301 gtagaaggtc acgcggttca ccgtcgcccg ctcggcgatg tcctgcacgg tgatggcctg
     9361 aaagctctgc tcatgctcgg cttggtgagg atcggcatga gtaagtctcc cttcgccggt
     9421 ggtcagggca ttggctggga gtttgagttc atcctggcag ccgtggcatt ggctctgctc
     9481 ttcctcggcg caggcagcat cgcgctcgat ccagtcgtag ggctgtagga gagacgcata
     9541 ccgaaagccc tcctcggcaa ccagggtgca ctgcccggga caaactcatg gcactcagac
     9601 tgaaagccct ggcccggctg atggccaagg ctgggggtga cgtgtgacaa ggaccggcgg
     9661 gaggtcaggc gggggcgaag gagacaggcg ggtataatag tgcgagcata gttcatttcc
     9721 tgatcttcca ggccccatca aagttatctc gacgccggag ggaaggaggg aggctgtgag
     9781 caaccaggat attcaagcca cctggcaata tcatgacggc accaagcatc ccgccgggcg
     9841 gctgatgaac cctcagcacc acttcgatcc cagtcgccag cccctgctat tcaaaatcta
     9901 caaggacctg ccgtctatct cactcccact tgacccctcg cccagtgtgc ccgcgttgga
     9961 ggccattgcg ggcgagatcc cggcagcagg cggcgagtcg gcgccagatt tgccg
//
LOCUS       DBNHXB010000081         5141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_350171, whole genome shotgun sequence.
ACCESSION   DBNHXB010000081 DBNHXB010000000
VERSION     DBNHXB010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_350171"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..2173
                     /gene="htpG"
                     /locus_tag="AC162G_03145"
     CDS             299..2173
                     /gene="htpG"
                     /locus_tag="AC162G_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HIR5074844.1"
                     /translation="MSQDTPSQSYEFRAEVRKLLDILVHSLYTEREIFLRELISNASD
                     ALNRIQFEMLTNRDVLDPDLEPVIQIDCDETARTLTVSDTGSGMTREELIENLGTLAR
                     SGVAAFLKQVESGAPIKADVIGQFGVGFYSVFMVAEEVKVVSRSYRRDAEACAWISRG
                     DETFRVEPAERAQRGTTVEIKLKEDAAEFASAWRLEQIVKKHSDFTSFPIKVKDRQAN
                     RQTALWRQSPREVTDEQYDEFYRSLTLDLEKPLARAHVSADAPVHIQAVLYVPGRRER
                     GILSLRRDDGLKLYSRKVLIQEYNKDLLPQYFRFVVGVVDSEDMPLNVSRESVQSNRV
                     VERVRTVLTNRLIDELERLAAEEPDKYRSFWKEFGPFLKEGISLDPAARAKLDKLLRF
                     PSSKVDGDRLTSLAQYVERMQPDQKAIYYLLGADVKSAARSPHLDYFRKHDLEVLYMV
                     DPLDSFLTTVLRDFQGKPLQNVDDAGLELPKDEPPAASEALSDEELKELLERFRTVLG
                     ERVVEVQEGKTLSDSPGRLVSPKDAVDRDMARVRRLLNQDYEVPKKIMEVNRAHPLTR
                     NLARLVIDSPDDPIIDVTIEQLYDSTLLVDGLHPNPAEMVGRIQQLMEAATDAKRISG
                     "
     gene            complement(2181..2630)
                     /locus_tag="AC162G_03150"
     CDS             complement(2181..2630)
                     /locus_tag="AC162G_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024861.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR5074845.1"
                     /translation="MSRANLFLDSSALFAGVVSPSGAARALLLLAEAGAITITISEQV
                     IAETERAIARKAPRALPYYREAVRRVGVRIVHDPSIEEVQAHRHIISHPADVPIVLAA
                     MKARADYLVTLNRRHFIDDPEVAARSGLHIGTPGDALAWVRAQLSAS"
     gene            complement(2617..2892)
                     /locus_tag="AC162G_03155"
     CDS             complement(2617..2892)
                     /locus_tag="AC162G_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015947.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR5074846.1"
                     /translation="MPAITVQIRRKGVITLPVELRRQYSLDEGDVLTLIDLGDGSFLL
                     TPRISQVARQGDRVSQIMAEEQVKLEDMLKALDEEREQYYQEHYVQG"
     gene            complement(2975..4927)
                     /gene="selB"
                     /locus_tag="AC162G_03160"
     CDS             complement(2975..4927)
                     /gene="selB"
                     /locus_tag="AC162G_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HIR5074847.1"
                     /translation="MHVIGTAGHVDHGKSTLVEALTGVHPDRLKEEREREMTIDLGFG
                     WMTLPNGEPVGIVDVPGHIDFIENMLAGVGGIDAALFVVAADEGVMPQTREHLAILDL
                     LKIEAGVVALTKTDLVDSEWLELVQGDVAESLRSTALDGAPIVPVSARRGDGIPELIH
                     ALQSVLAQTPARLDRGRPRLSVDRVFSIAGFGTVVTGTLIDGSLGVGDEIEILPRGLA
                     ARIRGLQTHKTKVERAVPGSRVAVNLSGVEVSDVKRGAVIVTPGWLRPTLLFDARFEH
                     LMPLQKPGFSSPPEARQRGSEKPGFPTERPLKHNDEVKVFHGAAEVTAHVRLIGDESL
                     PPGRAGWVQFALREPLAVVKGDRFIVRLPSPSITLGGGVVVDAQPGRRHRRFRPDVIA
                     KLETLAQGSPTELFLQALDASGPIVTTDLLKRANLPADAASQALAELEATGDVLKIGG
                     ASAVVASRAGWNAMRSRLASELGAYHKAHPLRGGMPREELKSRLRLDARVFNAIVSQA
                     AADGALVESQTSLCLPNFAVTFTPAQQKSIDALLARFTSQPWAAPSVKESEAAVGADV
                     LAALIELGQLVKLSEDVLLLLETYRAAVTRIEDHLQANRSITVAQARDLFDTSRKYAL
                     ALMEHLDAKGITKRVGDERVMGTKGT"
BASE COUNT          938 a         1600 c         1637 g          966 t
ORIGIN      
        1 ggcgctcatc gtttctgttt tgtagatgcc ggagcgcttg cgaaagttcc cttttggcat
       61 ctaacctcag tactgtttgg ttagcgggcc gggctcccac gcccggcggc atgggagccg
      121 cctccgcagc cttatccaaa cagtgttgcg tctaaccttg cgaaggttcg cggaccctcg
      181 caaggttggc gaccggctct aatctcgttc tagcacaact gggctataat ccattctgtc
      241 acgcaagtcc aatccaactt ccaatctcca gcctccaact tctagccgga ggagcaccat
      301 gagccaagac actccctccc aatcttacga attccgcgcc gaggtgcgga aattgctgga
      361 cattctggta cattcactgt atacggaacg cgagattttc cttcgcgaat tgatctccaa
      421 cgcttcggac gcgctcaacc ggatacagtt cgagatgctt accaaccgcg acgtgctcga
      481 tcccgacctg gagccggtga tccagatcga ctgcgacgag acggcgagga cgctgaccgt
      541 ctccgatacc ggcagcggca tgacacgcga ggagttgatc gagaacctgg gcacgctggc
      601 gcgctcgggc gtggcggcgt ttctgaagca agtggagtca ggtgcgccga tcaaggccga
      661 cgtcatcggc cagttcggcg tggggttcta ctcggtcttc atggtcgccg aagaggtcaa
      721 agtcgtctcg cgctcgtatc gccgggacgc cgaggcgtgc gcctggatct cgcgcgggga
      781 cgagaccttc cgcgtcgagc cggccgagcg cgcgcagcgc gggacgacgg tcgagatcaa
      841 gctcaaggag gacgcggccg agttcgcttc ggcgtggcgg ctggagcaga tcgtcaagaa
      901 gcactccgat ttcacgtcgt tcccgatcaa agtgaaggac cggcaggcca accggcagac
      961 ggcgctgtgg cgccagtcgc cgcgcgaggt gacggacgag cagtacgacg agttttaccg
     1021 atcgctcacg ctcgacctgg agaagcccct cgcgcgtgcc cacgtctcgg ccgacgctcc
     1081 ggtgcacatc caggccgtgc tgtacgtgcc gggccggcgc gagcgcggaa tcctgagcct
     1141 gcgcagggac gacggcttga agctgtactc gcgcaaagtg ctgatacagg aatacaacaa
     1201 ggacttgctg ccccagtatt tccgtttcgt cgtcggcgtc gtcgattcag aggacatgcc
     1261 gctcaacgtc tcgcgcgaat ccgtgcagag caaccgcgtc gtcgagcggg tgcgtacagt
     1321 cctgaccaac cgcctgatcg acgagctgga gcgcctggcc gccgaggaac cggacaaata
     1381 tcgaagcttt tggaaggagt tcgggccgtt tctcaaggaa ggcatctcgc tcgatccggc
     1441 cgcgcgggcg aaactcgaca agctgctgcg ttttccgtct tccaaggtag atggcgacag
     1501 gctcacgtcg ctggcgcagt acgtcgagcg catgcagccg gatcaaaagg cgatttacta
     1561 tctgcttggc gccgatgtca agtcggcggc acgcagcccg cacctcgact acttccgcaa
     1621 gcacgacctc gaagtgctgt acatggtcga tccgctcgac agtttcctga cgaccgtcct
     1681 gcgcgatttc cagggcaagc cgctgcagaa cgtcgacgat gcggggttgg aactaccgaa
     1741 agacgagccg ccggctgcga gtgaggcgct gagtgacgag gaactgaagg aactcttgga
     1801 acgattccgc acggtgctgg gcgagcgcgt cgtcgaagtg caggaaggca agacgctgag
     1861 tgacagtccc ggccgtctgg tctcgccgaa ggatgcggtc gaccgcgata tggcgcgggt
     1921 gaggcggctg ctgaaccagg actacgaggt gcccaagaag attatggaag tgaatcgcgc
     1981 gcacccgctg acgcgcaacc tggcgcgctt agtgatagat agcccagacg acccgatcat
     2041 cgacgtgacg atcgagcagc tttacgacag cacgctcctg gtggatggat tgcatccgaa
     2101 tccggcggaa atggtggggc ggatccagca gttgatggag gcggcaacgg atgcgaaacg
     2161 gataagcgga tgagtgcaat tcacgacgcg gatagctgtg ctcgcaccca ggctagcgcg
     2221 tcccctggcg tgccgatgtg taagcccgag cgggccgcca cttcgggatc gtctatgaaa
     2281 tgccgccggt tcagggttac cagatagtca gccctggcct tcatggctgc gaggacgatc
     2341 ggcacatctg ccgggtgtga gatgatgtgc cggtgagcct gcacctcttc gatggacggg
     2401 tcatgcacga tgcgcacacc gacgcggcgg acggcctctc ggtagtaggg caatgcgcgg
     2461 ggagccttgc gggctatggc tcgctctgtc tcggctatca cctgttcgga aatcgttata
     2521 gtgattgctc ccgcctctgc cagcagcagt aaggcgcggg ctgctccgct gggagaaaca
     2581 acgccggcga acagggcgct gctgtccaga aacaggttag ccctggacat agtgttcttg
     2641 ataatactgc tctcgttctt cgtccagggc tttcagcatg tcttccagct taacctgctc
     2701 ctcggccatt atctggctaa cccggtcacc ctgacgggct acttgggaga tgcgaggcgt
     2761 cagcaggaaa gagccatcgc ctagatcgat cagggttaag acgtctccct catccagact
     2821 gtactggcga cgcagttcca ctggtagtgt gataaccccc ttgcgacgaa tctggacggt
     2881 gattgcgggc atagcctgtc ctcaaaatgg aagaatttcc gaatttctgc cttgataata
     2941 gcctattgcg ccagcaaagt caagtatggt tcccttaagt tccctttgtt cccatcaccc
     3001 tttcatctcc cacgcgcttc gtaattccct tcgcatccag gtgctccatc agcgccagcg
     3061 catatttgcg gctcgtatca aacaaatcgc gcgcctgcgc gacggtgatc gatcgattcg
     3121 cctgcaagtg gtcctcgatc cgggtcaccg ccgcccgata cgtctccagc agcagcagca
     3181 cgtcctcgct cagcttcacg agttgcccca gttcgatcag cgccgcgagc acgtctgcgc
     3241 cgaccgccgc ctcggactcc ttgactgacg gcgcggccca cggctgcgat gtgaatcggg
     3301 ccagcagcgc gtcgatcgat ttctgctgcg caggcgtgaa tgtcacggcg aaattaggca
     3361 ggcagagact tgtctggctt tcgaccaatg cgccgtccgc tgccgcctgc gagacgatgg
     3421 cgttgaacac cctggcgtcg agcctcaagc ggctcttcaa ttcctcacgt ggcatgccgc
     3481 cccgcaaggg gtgagctttg tgataggcgc ccagctcgct cgccagacgg gatcgcatcg
     3541 cgttccagcc tgcgcgcgac gcgacgacgg cggatgcgcc gccgatcttc agaacgtctc
     3601 ctgtcgcttc taattcggcc agcgcctggc tcgccgcgtc ggcgggcagg ttggcgcgct
     3661 tcaggagatc ggtagtcacg attggcccgc ttgcgtcgag cgcctgcaag aacagttcgg
     3721 tgggcgagcc ttgcgccagc gtttcaagtt ttgcgatcac atcgggccgg aagcgccgat
     3781 ggcggcggcc gggctgcgcg tctacgacga ctccgccacc cagcgtgatc gagggcgagg
     3841 gcaggcgcac gatgaagcgg tcgcccttca cgactgcgag cggctcccgc agcgcgaact
     3901 gcacccatcc ggcgcgcccg ggcggcagac tttcgtcgcc gatcaggcgt acgtgagcgg
     3961 tgacttcggc tgcgccatgg aagactttga cttcgtcgtt gtgcttgagc ggccgttcgg
     4021 tggggaaacc cggtttctcc gatcctctct gccgcgcttc gggcggactc gagaaaccgg
     4081 gtttctgaag cggcatcaga tgctcgaagc gcgcgtcgaa cagcagggtg gggcgcagcc
     4141 aacccggcgt gacgatgacg gcgccgcgct tcacgtcgct cacctcgacg ccgctcaggt
     4201 tgaccgcgac gcggctgccg ggcacggcgc gctcgacttt cgtcttgtgc gtctgcaagc
     4261 cgcgaatgcg cgccgccagc ccgcgcggca gtatctcgat ttcgtccccg acgccgagcg
     4321 agccgtcgat cagcgtgcct gtgacgaccg tgccgaatcc cgctatcgag aacacgcgat
     4381 cgacggacag gcgcgggcgc ccacggtcga ggcgggccgg cgtttgcgcc agcacgctct
     4441 gcaaagcatg gataagctcg ggaatgccgt cgccgcgccg ggccgagaca gggacgatcg
     4501 gcgcgccgtc gagtgcggtg ctgcgcagcg actcggctac gtcgccctgc acgagttcca
     4561 gccattcgga atcgaccaga tcggtcttgg tgagggcgac gacgccggct tcgatcttga
     4621 ggaggtcgag gatggcgagg tgctcgcgcg tctgcggcat cacgccttcg tcggcggcga
     4681 cgacgaacag cgccgcgtcg atgccgccca cgccggccaa catgttctcg atgaagtcga
     4741 tgtgcccggg cacgtcgacg atgcccaccg gctcgccgtt cggaagcgtc atccagccga
     4801 agccgaggtc gatcgtcatc tcgcgctcgc gctcttcctt gagccggtcg ggatgcacgc
     4861 cggtgagcgc ttccaccagc gtggacttcc cgtgatcgac gtggccggcg gtgccgatga
     4921 cgtgcataaa ggagttcctt cagttccctg agttcctcac ggggatcctg ggaagtcaag
     4981 gaactcaggg aacccaggga agtaactgga catgcctcgc gcgatgatca actgcgcttg
     5041 cggccccggg ggtcgggaat gcccaaccca tcctcgggcg aggtggccgg cggcacgctg
     5101 acgttgggca gcgtgctctt gcgcgtctcg gtcaggccgg t
//
LOCUS       DBNHXB010000082         7256 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_853801, whole genome shotgun sequence.
ACCESSION   DBNHXB010000082 DBNHXB010000000
VERSION     DBNHXB010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7256
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_853801"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1150
                     /locus_tag="AC162G_03165"
     CDS             161..1150
                     /locus_tag="AC162G_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5074848.1"
                     /translation="MNNRIVRWGLLSTARINRALIPPLRASARNELIAVASRDLTKAR
                     TYAEEWNIPHAYGSYDELLADPEIDVIYNPLPNSLHAEWTIKTAQAGKHVLCEKPLAV
                     SVDEVDVLSAAAKQAGVVVAEALMYRHHPQTLQVKELVDAGAIGTLRLVRGSFTFNLT
                     RTTDVRLDRALGGGSIWDVGCYPISYARYVVGAEPVEVYGWQIAGPSGVDETFVGQMR
                     FPNGVYAQFDCSFRAAFRTHMEIVGSDGTISMPHPFRPGLNERISVARGDDVQTITIP
                     GQELYIGEVEDMADAVLLGRPPRVSLADSRGNVAAIIALLRSARESRPIPVGN"
     gene            1385..2668
                     /locus_tag="AC162G_03170"
     CDS             1385..2668
                     /locus_tag="AC162G_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5074849.1"
                     /translation="MSRSKRWLSVAGSLLIIGSVVLSACQPAAPAVSQKLEFFSWWTA
                     GGEAEGLNGMYEVYKAENPGIEIVNATVAGGAGTNAKAVLATRLTGGDPPDSFQVHAG
                     LEVQTYAPEQYLEPLDDLYASEGWDKVFPGDLLTLLKYQDHYWSVPVNIHRSNVLWYN
                     KKVLEDNGLQPPETFDEFFAAADTLKAKGIFPLVMGTKDGWEAGHVFEDVLAGTLGAD
                     AYRGLWTGATPWTDAKVTQALENYKKMLSYVNEDNSALTWDGAGQYLIDGQGAMMIMG
                     DWTAGWFASKKYADYGWAPPPGTQGIFVALSDSFALPKGAKNRDSALGWLKVSGSKDG
                     QEAFNPKKGSICARTDCANDAFKTVPETYEYLTSAAQDWQRDKIVPSVVHGAASFESW
                     ATEYKDIIALFVQSGDVAGTQRSLQQACVNAGICK"
     gene            2854..3771
                     /locus_tag="AC162G_03175"
     CDS             2854..3771
                     /locus_tag="AC162G_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5074850.1"
                     /translation="MRRLTRDRIISIILILPSVLCIALFVYGFIGYTGFVSFTKWSGQ
                     LIPDYTLVGLGNYQRLFDINDVAGQRFLKDLSNTAWFTVLFLIATIVIGFLLAVLLDQ
                     RIRAEGLFRNIYLFPIAISFIVTGVVWRWMLAPGTEATGAAGVNLLLEKLGLGFLKSG
                     WYTDPSIGIKAVVIAATWQMSGYTMALYLAGLRSIPDELREAARVDGASEFQIYRYVM
                     IPLLWPITLSAVIILGHISLKIFDLIVAMTGPGTGFSTDVPALFMFDTTFRGNQFAQG
                     ASIAIVLLLMVAVLVVPYLSYSSRREAER"
     gene            3768..4727
                     /locus_tag="AC162G_03180"
     CDS             3768..4727
                     /locus_tag="AC162G_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5074851.1"
                     /translation="MTAQPVRKSFRRSRLLIYLFLIGAAAVFLLPVYVLVVTGFKSFQ
                     EVSLATMWNPPSAFSLASFQKAWSGSEAEGFRGLAGNFLNSVYLAVPATLLSAILGSI
                     NGYILSKWKFRGADTLFSLMLFGMFIPYQSVLIPLVQVLQQLSRVTGPIAEAIASMSV
                     PAALSGLPPWLAGFVPAYGTIGGLILVHVIYGIPITTLIFRNYYTAVPTELVEAAKLD
                     GASLLGIYRWVLFPISMPGFVVVLIWQFTSIWNDFLFGVIVTSRPAVQPVTVALNNLA
                     GSYIVEWNVQMAGAFLTALPTLLVYILLGRFFMRGLLAGSLKG"
     gene            4808..5365
                     /locus_tag="AC162G_03185"
     CDS             4808..5365
                     /locus_tag="AC162G_03185"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF024969.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose-7-phosphate isomerase"
                     /protein_id="HIR5074852.1"
                     /translation="MDNDFFAGYFDEVHASLRSVARDRIAAVVALLRRVRTERRRVFI
                     FGNGGSAATSSHFACDLIKLTRQQGLPDFRVIALTDNMPVFSAFANDHGYDTVFAAPL
                     KSLAESGDVAIGISASGRSANVLRGMDVAQELGLFKVGFAGYDGGLLKDKVDVCLIAP
                     GKHASQIEDAHHVLQHAICRAIIET"
     gene            5378..6352
                     /locus_tag="AC162G_03190"
     CDS             5378..6352
                     /locus_tag="AC162G_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016873672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR5074853.1"
                     /translation="MESRVLLALDFGGTKHSAAIAAIGSREWQALQRVQSPPAPNVHT
                     DMALMLGLARDLLAGRTLAAIGVSFGGPVNAAMGTVLLSHHVPGWENVPLQDQLRSEF
                     NVPVSVDNDANVAALGEWQFGAGQGCASLLYVTVSTGVGGGWVLDGRVFHGTDSMAGE
                     IGHIIVQPDGPACVCGKRGCVEAIAAGPNLARRARERLAQAVDQETRPHQAGHILREL
                     VGGDLSAVRAVQVARAAQAGDPLACEVLDEAARALGLGLGTAITLMNPQRVVLGGGVT
                     KSGERYWRTVRDTARETVLPGMTIDIVPAALDDDAPLWGAIALAERLL"
     gene            6352..7176
                     /locus_tag="AC162G_03195"
     CDS             6352..7176
                     /locus_tag="AC162G_03195"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5074854.1"
                     /translation="MAYLDGTAIELVRSDIPRGHIRVALFDFDGTLSLIREGWQEVMQ
                     SLMVEILLQTPRHESVDELRAVATELIDRLTGEQTVNQMAGLALEVARRGGRPANPQE
                     YKRQYLDRLRERIEPRVAALKSDQVVPDEALVPGTRALLESLRSWGVTCYLASGTDEV
                     AVRGEATALGIAGYFAEIRGARDGDPRASKNAVIEQIMREHSLRGEEFVAFGDGCVEI
                     ESTKRVGGLAVGVASDEVKRRGVNRRKRERLIQAGADIIVPDFDEHRELIAFLFGL"
BASE COUNT         1253 a         2203 c         2350 g         1450 t
ORIGIN      
        1 tgcaggcgcg tggggccacc gtagatcttc gggtctatcc cgggcagggg catatgttcc
       61 ggggctgggc gcacaccgac gcttggacgc gtacgctggc ctggttcgcc agatacgtac
      121 gtggccagcc atagcgtgat gagcaagggg ctaagttctt atgaacaatc gtattgtgcg
      181 ctggggcctc ctcagcacgg cgcgcattaa ccgcgccctt atcccgccgc tgcgcgcctc
      241 agcgcgcaac gaactcatcg cggttgccag tcgggacctg acaaaggcga ggacttatgc
      301 tgaggagtgg aacatcccgc acgcttatgg cagctacgac gagctgctgg ccgatccgga
      361 gatcgacgta atctacaacc cgctgcccaa cagcctccat gccgagtgga cgatcaagac
      421 ggcgcaggcg ggcaagcacg tgctgtgcga gaagcctctg gccgtgtctg tcgacgaagt
      481 cgacgtgctg agcgccgcgg cgaagcaggc gggcgtcgtc gtcgccgagg ccctcatgta
      541 tcggcaccac ccgcagacgc tccaggtgaa agaactggtc gatgccggag ccatcgggac
      601 gctgcggctc gtgcgcggtt cgttcacctt caacctcacc cggacgaccg acgtgcgact
      661 ggatcgggcg ctgggaggcg gcagtatctg ggacgtgggt tgttacccga tcagctatgc
      721 gcgatacgtt gtgggcgcag agcctgtcga ggtgtacggc tggcagatcg ccgggccaag
      781 cggcgtcgac gagactttcg tcgggcagat gcgctttccg aacggcgtgt atgcgcagtt
      841 cgactgcagt ttccgtgccg cgttccgcac gcacatggaa atcgtgggca gtgatgggac
      901 gatcagcatg ccgcatccgt tcaggccggg actgaacgag cgcatttcgg tggcgcgcgg
      961 cgacgatgtt caaacaatca caatccccgg ccaggagctg tacattggcg aagtcgagga
     1021 tatggctgac gccgttctgc ttggccgccc gccacgcgtg agcctggccg acagccgtgg
     1081 caacgtggcg gcgatcatcg cgctgctacg ttcggcgcgt gaaagccgtc ctatcccggt
     1141 tgggaattag cccgtgcgca taccgccgta tcttgactca gctatgcgcc agtgctatgc
     1201 tatggatagc ctcgtgcacg cttaaggtcc cgacttgggg gttccgccga tcgtcccgcg
     1261 cggtggatgg gttatcgcga aaggaggtga ggttcgggcc tatgattccg ctgagacagc
     1321 gccgtccaac ccgcttgttt ctgttcgttt ccgtctgtcc cattcctgat caggaggaaa
     1381 tgaaatgtcc cgctcgaaac gctggctttc cgtcgcaggg agcctgctta tcattggctc
     1441 ggtcgtgctg agcgcctgcc agccggcagc gcctgccgtg agccagaaac tggagttctt
     1501 ctcgtggtgg accgccggtg gcgaagctga gggtcttaat ggcatgtacg aggtgtacaa
     1561 agccgagaat cccggcattg agatcgtcaa tgctaccgtt gccggcggcg cgggcaccaa
     1621 cgccaaagcg gtgctcgcca cgcgcctgac cggcggtgat ccccccgact ccttccaggt
     1681 gcacgccggc ctggaggtcc agacgtacgc gcctgagcag tatctggagc cgctggacga
     1741 cctgtacgct tccgaaggct gggacaaggt cttccccggt gacctgctta cgctgctcaa
     1801 gtatcaggat cactactggt cggtgccggt gaacatccat cgctcgaatg tgctgtggta
     1861 caacaagaag gtcctcgagg acaatggcct ccagccgccc gagacgttcg atgagttctt
     1921 cgccgcagca gacacgctca aggcgaaggg catcttcccg ctcgtcatgg gtacgaaaga
     1981 cggctgggag gccgggcacg tcttcgagga tgttctcgcc ggcacgctgg gcgccgacgc
     2041 ctatcgcggt ctgtggacgg gtgccacgcc gtggacggat gccaaggtga cccaggcgct
     2101 cgagaactac aagaagatgt taagctatgt caacgaggat aattctgcgc tgacgtggga
     2161 tggtgccggg cagtacctga ttgacggcca aggcgcgatg atgatcatgg gcgactggac
     2221 ggccggctgg ttcgccagca agaagtatgc cgattacggg tgggcgccgc cgcccggcac
     2281 tcagggcatc ttcgtcgcgc tgtccgactc gttcgcgctg cccaagggcg ctaagaaccg
     2341 tgatagtgcc ctcggctggc tgaaggtgtc cggctcgaag gacggccagg aagccttcaa
     2401 ccccaagaaa ggctcgatct gcgcgcgcac cgattgcgcc aacgatgcct tcaagaccgt
     2461 cccggaaacg tacgagtatc tgacctcggc ggcccaggac tggcagaggg acaagatcgt
     2521 gccgagcgtg gtgcacggcg cggcgtcctt cgagagctgg gcgaccgaat acaaggacat
     2581 catcgccttg ttcgtccaga gcggtgatgt cgccggcacg cagaggtccc tgcagcaggc
     2641 gtgcgttaac gccggcatct gcaagtaacc tgctggcgac ggcgaggcga cccgcctgga
     2701 ggcaggccgc ctcgccttct caaagcctat ccccaaaccc cagccgaggc ggccgcgtgc
     2761 gcagcctccc cgtgggacat gccgccagat ggctgggcgt gggagcccgg cccgctgctc
     2821 tttcggatag gcacttcatc tgggagtctt tctatgcggc gcctgactcg cgatcggatc
     2881 atctcgatca tcctcattct tccctccgtc ctatgcatcg cgctcttcgt ctacggcttc
     2941 atcggttaca caggctttgt ctcattcacc aagtggagcg ggcagcttat ccccgactac
     3001 acactcgtcg gcctgggcaa ttatcagcgc ctgttcgata tcaacgatgt cgccgggcag
     3061 cgcttcctca aagatctgag taacaccgcc tggtttacgg tcctgttcct gatcgccacg
     3121 atcgtcatcg gtttcctgct ggcggtgctc ctcgaccagc gcatccgggc ggagggccta
     3181 ttccgcaata tttacttgtt cccgatcgca atctcgttca tcgtcaccgg cgtcgtctgg
     3241 cgctggatgc tggcgcccgg cactgaagca accggggcgg cgggagttaa cttgcttctg
     3301 gagaaactcg gtctgggctt tctgaagagc ggctggtaca ccgatcccag catcggcatc
     3361 aaggcggtgg tcatcgcggc aacgtggcag atgtccgggt acacgatggc tctgtacctg
     3421 gcggggctgc gcagcatacc cgatgagctg cgcgaagcgg cgcgcgtcga tggcgcgagc
     3481 gaatttcaga tctaccggta cgtcatgatc ccgcttctgt ggcccattac actcagcgcg
     3541 gtcatcatcc tgggacacat ctcgctgaag atattcgact tgattgtggc gatgaccggc
     3601 ccgggcacgg gcttctcgac cgacgtgcca gccctgttca tgttcgacac gacctttcgc
     3661 ggcaaccagt tcgcgcaggg cgcatccatt gccatcgtgt tgctgctgat ggtggccgtg
     3721 ctggtcgtgc cctatctcag ctattcctct cggcgcgagg ccgaacgatg actgcgcaac
     3781 cggttcgcaa atcgttccgc cggagccggc tgctgatcta cctgttcttg atcggcgcgg
     3841 cggccgtttt cctgctaccg gtgtacgtgc tggtggtcac gggtttcaag tcgttccagg
     3901 aagtcagcct ggccactatg tggaatccgc cttccgcatt cagcctcgcc tctttccaga
     3961 aggcgtggtc cggcagcgag gccgaaggct ttcgtgggct ggccggcaac tttctcaaca
     4021 gcgtgtacct ggccgtgcct gcgacgctgc tgtcggccat cctcggctcg atcaacggct
     4081 atatcttgtc gaaatggaaa ttccgaggcg cggatacgtt attctcgttg atgctcttcg
     4141 gaatgttcat cccgtatcag agcgttctca tcccactcgt gcaggtgctg cagcagctct
     4201 cccgggtgac cgggccgatc gccgaagcga tcgcctcgat gtccgtcccg gccgcgctga
     4261 gcgggcttcc tccgtggctg gccggtttcg tgccggccta cggcaccatc ggcggactga
     4321 tcctcgttca tgtcatctat ggcatcccca tcacgacgct gatcttccgc aactactaca
     4381 ccgccgttcc aaccgagctg gtcgaagccg ccaagctcga cggcgcctcg ctgctgggca
     4441 tttatcgctg ggtgttgttc ccgatctcga tgcccggctt cgtcgtcgtg ctgatctggc
     4501 agttcacgtc gatctggaac gatttcctat tcggcgttat cgtcaccagc cggccggcgg
     4561 tgcagccggt gacggtcgcg ctcaacaacc tggccggatc gtacatcgtc gagtggaacg
     4621 tgcagatggc gggcgcgttc ctcaccgcgc tgcccacgct gctggtctac attctgttgg
     4681 gccgtttctt catgcgcggg ctgctggccg ggtcgctcaa gggttgaggc tgccgtttca
     4741 ccgccgagtg gccatagcgt ttgtcacgcc acaccgggag cggtggggtg tattcgtgga
     4801 gcgtgtcatg gacaacgatt tctttgcggg ttacttcgac gaagtgcacg catcgctgcg
     4861 ttccgttgcg cgcgaccgaa tcgcggcggt cgttgccctc ctgcggcggg tgcgaaccga
     4921 gcggcggcgc gtgttcatct tcggcaacgg cggcagcgcg gcgacgtcct cccacttcgc
     4981 ttgcgacttg atcaaactca cgcgccagca ggggttgccg gatttcaggg tgatcgcgct
     5041 cacggataac atgccggtgt tctctgcctt cgcgaatgac cacggctacg acaccgtatt
     5101 cgccgcgccc ctcaagtcac tggccgagtc gggcgacgtc gcgatcggga tcagcgccag
     5161 tggccgatcg gccaacgtgc tgcgcggcat ggacgtcgcg caggagcttg gcctgttcaa
     5221 ggtgggcttc gccggatatg atggcgggct gttgaaggac aaagtggatg tctgcctgat
     5281 tgcaccgggg aagcatgcgt cgcagatcga ggacgcgcat cacgtgctgc agcatgcgat
     5341 ctgccgggcg atcatcgaga cctgaggacg cggcacgatg gaatcgcgcg tattactggc
     5401 gctggatttc ggcggcacga aacactcggc cgcgatcgcc gcaatcggat cgcgagaatg
     5461 gcaggctctg caacgagtcc agtcgccgcc tgcgccgaat gtgcacaccg acatggcgct
     5521 gatgttgggc ctggcgcgcg atctcctggc cggccgcaca cttgcggcga tcggcgtgag
     5581 tttcggcggg ccggtcaatg ccgccatggg gacggtgctg ctgtcgcatc acgtccccgg
     5641 ctgggagaat gttcctctgc aagaccaatt acgatccgag ttcaatgtgc ccgtgagtgt
     5701 ggacaatgac gcaaatgttg ccgcgctggg ggagtggcaa ttcggcgcag gccaggggtg
     5761 cgccagcctt ctctacgtga ccgtcagcac cggcgtcggc ggcggttggg tgctggacgg
     5821 gcgcgttttt cacggcactg acagcatggc cggcgagatc ggacacatca tcgttcagcc
     5881 cgacggcccg gcttgcgtgt gcggaaagcg aggatgcgtc gaagctatcg ccgctggccc
     5941 gaacctggcg cggcgcgcac gcgagcgcct ggcccaagcg gtcgatcagg agacccgacc
     6001 ccatcaagcg ggtcacattc tgcgtgaact cgtcggcggt gatctgagtg cagtgagggc
     6061 tgtgcaggtc gcccgcgctg cccaggccgg cgatccgctt gcatgcgagg ttctcgacga
     6121 agcggctcgc gcattggggc ttggactggg cacggcaatc acgctgatga atccacagcg
     6181 cgtcgtgctc ggcggcggag tgactaagtc cggggagcgc tactggcgca ccgtgcgcga
     6241 tacggcacgc gaaacagtgc tgcccggcat gaccatagac attgtgccgg cagccctgga
     6301 tgatgacgcg ccgctgtggg gagctatcgc actggccgaa aggctacttt gatggcctat
     6361 ctcgacggga ctgcgatcga actcgtgcgc agtgacattc cacgcgggca tattcgcgtg
     6421 gcgctgttcg atttcgacgg caccctttcg ctcatccgcg aaggctggca ggaggtgatg
     6481 cagtccttga tggtcgagat cctcctgcaa acaccacggc atgaaagtgt cgacgaactg
     6541 cgagccgttg ccacagagtt gatcgatcgg ctcaccggcg agcaaacggt caaccagatg
     6601 gccgggttgg cactggaagt ggcccggcgt ggtgggcggc cggcaaaccc acaggaatac
     6661 aaacggcagt atctcgatcg actgcgggag cgaatcgaac cccgtgtcgc cgccctcaag
     6721 tctgatcagg ttgtccccga tgaagcgctc gtccctggca cacgcgcgct gctcgagtcc
     6781 ctccgttcgt ggggcgtcac gtgctatctg gcatcgggca ccgacgaggt ggctgtgcgc
     6841 ggcgaggcga cggccctggg catcgccggt tacttcgcgg aaattcgcgg cgcgcgggac
     6901 ggcgatccac gtgcatcgaa aaatgccgtg atcgagcaga ttatgcggga gcatagcctg
     6961 cgcggcgagg agttcgtcgc gttcggcgat ggttgcgtcg agattgagag cacaaaacgc
     7021 gtgggtggcc tcgcggtcgg cgtggcgtca gatgaggtta agcggcgtgg cgtaaatcgc
     7081 cgtaagcgtg agcgcttgat tcaggcggga gcggatatta tcgttccaga ttttgatgaa
     7141 cacagagaac tgatcgcttt cctgtttggc ttgtgacgaa gatctttcac gtctcccaag
     7201 cttgcccgat cggttcggga gaatcgcatg tcctacgcga ggttcgaccg tagtcg
//
LOCUS       DBNHXB010000083         4097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1189692, whole genome shotgun sequence.
ACCESSION   DBNHXB010000083 DBNHXB010000000
VERSION     DBNHXB010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1189692"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3733)
                     /locus_tag="AC162G_03200"
     CDS             complement(<1..3733)
                     /locus_tag="AC162G_03200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR5074855.1"
                     /translation="MSFVHLHVHSEYSLLDGLNKPAHIVKRAKELNQPAVALTDHGVM
                     YGAIDFYNAARQAGVKPLIGMEGYLARRSRHNRDPQLDKSPHHLLMLAQNETGYKNLL
                     QLASISQLEGFYYRPRVDKEVLERFSEGLILTTGCGSAEVPRLIRDGAIDTAREALAW
                     YVDVFGRERFFVELQLHDGIPDLVQVNRQLLALGQAFGLRPVATNDVHFLRAENAHAQ
                     DVLLCIGTGSLVSEPDRMKMTDDSYYLKSADEMAALFGEIPEALSNTLLVAEMCEVDL
                     GFQGYHLPDFPVPEGHTAETYLRAVCERGLAERYGERANDPEVRQRLDYELSVIHQMG
                     FDTYFLIVSDLTRYARESGIWWNVRGSAAGSIVAYTAGITLIDPLSHGLIFERFLNPG
                     RISMPDIDLDFPDDRRGEMIEYAVRKYGRDKVAQIITFGTLGARAAIRDVGRVLDMPL
                     PDVDRVAKLVPAIPGKPITIREAIEQVPELKQMCESTDYIRTLLRAAMEVEGAARSVG
                     THAAGVIISDRPLVEYVPLHRPTKGHGDSPLEAITQFEMHVCEEIGLLKVDFLGLSTL
                     TIMRKACDLIEQRHGVKWALDTIPTNDPAAFELMARGDVLGLFQVEGEGMRRVLTDMK
                     PTRFEHIVATLSLFRPGPMEYIPSFIRRMHDEEPIEYKHPALEPILAETYAIIVYQEQ
                     IIEIAKQLAGYSPPEADEMRKAVGKKIKEKIDEHRAKFITGAVANGIPESTAKAIYDD
                     IEFFARYGFNKAHATDYAVITCQTAFLKAHYPVEYMTALLTVERNNTQKIGLLIVEAR
                     RMGIDVLPPDVTRSQIDFTVERSGDHDAIRFGLAAIKNVGEGPVRAIIEARDPSPFDL
                     RPGGSGGSRFFNLDDFCRRVDLRQVNRRALEALIKVGAFEAFGNRAQLLASIDKMLGN
                     SSAAHRAQDVGQMSLFGDTGAGVSTMGTLPAIEEAPLRERLEWEKELIGVYISEHPVA
                     RVLAQARDTVSHLCGEIGEEHHGQKVALLGLVAAYRTTQTKKGDQMAFVTLEDIQGAV
                     DVVVFPKLWEQTRSLWQRDKIVLVRGKVDARGRKPSIICDSVTDNLDFVRPADAPTKD
                     PIALARSQSPTIQAAAKTREPRPAYGVPPRIQPAQPSVFAAATHATVQTAGPSTPVRA
                     ALPALHTPAHVDSAASSDDLALRAGAARHLRVVIQRTQDSRGDARRIGDAHRLLLSYA
                     GDDRFVFLVTGGSSGNYELAFPNDTTLICDELMARLQNLL"
BASE COUNT          688 a         1274 c         1337 g          798 t
ORIGIN      
        1 caagcaggtt ctgtaagcgt gccatcaact cgtcgcagat cagcgtcgtg tcgttgggga
       61 aagccaactc gtagttgccg ctgctgccgc ccgtgaccag aaacacgaat cgatcgtcgc
      121 ccgcgtaact cagcagcagg cgatgcgcat cgccgatgcg tcgggcgtcg ccgcggctgt
      181 cctgtgtgcg ctggatgacc acacgcaggt ggcgagccgc gcccgctcgc agggcgagat
      241 cgtcgctcga agctgcgctg tcgacgtgcg cgggcgtgtg cagcgccggg agcgcagcgc
      301 gcacaggtgt gctggggccg gcggtctgca cagttgcgtg agttgccgcc gcaaacacag
      361 acggttgagc cggttgtatc cggggcggta cgccgtaggc tggacgtggc tcgcgagtct
      421 tcgccgccgc ctggatcgtc ggcgattggc ttctggccag agcaatcggg tccttcgtcg
      481 gggcgtctgc aggccgcacg aaatcgagat tgtcagtgac cgagtcgcaa atgatcgacg
      541 gcttgcgtcc gcgcgcgtcg accttgccgc gcaccaggac gattttgtcc cgctgccaca
      601 gcgatcgcgt ctgctcccac agctttggga agacgaccac gtcgacggcg ccctggatgt
      661 cctcgagtgt gacgaacgcc atctgatcgc ccttcttcgt ctgcgtcgtg cggtaggcag
      721 caaccagccc cagaagtgcc actttctgac cgtgatgctc ctcgccgatc tcgccgcaca
      781 ggtggctgac ggtatcgcgc gcctgtgcca gcacacgagc aacggggtgc tccgaaatgt
      841 agaccccgat cagctctttc tcccactcca accgttcgcg cagcggcgct tcctcgatcg
      901 cgggcagcgt ccccatcgtc gacacgccgg cgcccgtgtc gccgaacagc gacatctggc
      961 ccacgtcttg cgcgcgatgc gccgcgctgc tgttccccag cattttgtca attgaggcga
     1021 gcagttgtgc gcggttgccg aacgcctcga atgcgccgac cttgatcagc gcctcgagcg
     1081 cgcgccggtt gacctggcgc agatcgacgc ggcggcagaa gtcgtctagg ttgaaaaatc
     1141 ggctgccccc tgagcctcct gggcgtaagt cgaagggcga agggtcacgc gcctcgatga
     1201 tcgctcggac ggggccttcg ccgacattct tgatggcggc cagcccgaag cggatcgcat
     1261 cgtgatcgcc ggaacgctcg accgtgaagt ctatctggct gcgcgtcacg tcaggcggga
     1321 ggacgtcgat gcccatgcgg cgcgcttcga cgatcagcag gccgatcttc tgggtgttgt
     1381 tgcgctcgac ggtcagcagc gcggtcatat actcgaccgg gtaatgcgcc ttgaggaacg
     1441 ccgtctggca ggtgatgacc gcgtagtccg tggcatgcgc tttgttgaat ccgtagcgcg
     1501 cgaagaattc gatgtcgtcg tagatcgcct tggcggtgga ttccggaatg ccgttggcga
     1561 ccgcgcctgt gatgaacttg gcgcggtgtt cgtcgatctt ctccttgatt ttcttgccga
     1621 ccgccttgcg catttcgtct gcttccggcg gcgagtaacc ggcgagttgc ttggcgatct
     1681 caatgatctg ctcctggtag acaataatgg cgtacgtttc ggccaaaatc ggctcgagcg
     1741 ccggatgctt gtactcgatc ggctcttcgt catgcatgcg ccggatgaac gagggaatgt
     1801 actccatcgg gccgggacgg aacagcgaca gcgtggcgac gatatgctcg aaccgggtcg
     1861 gcttcatgtc agtcagcacg cggcgcatgc cctcgccttc cacctggaac aggccgagca
     1921 cgtcgccgcg cgccatcagc tcgaacgccg cgggatcgtt cgtcgggatc gtgtcgagcg
     1981 cccacttcac gccgtggcgc tgctcgatga ggtcgcacgc cttgcgcatg atcgtcagcg
     2041 tggacaggcc gaggaagtcg accttgagca ggccgatctc ttcgcacacg tgcatttcga
     2101 actgggtgat cgcttcgagc ggcgaatcgc catggccctt ggtcggccgg tgcagtggca
     2161 cgtactcgac cagcggccgg tcggagatga tcacgccggc ggcgtgcgtg ccgacgctgc
     2221 gcgccgcgcc ttcgacttcc atggctgcgc ggagcagcgt gcggatgtag tcagtggact
     2281 cgcacatctg cttaagctcg ggcacctgct cgatcgcctc gcggatcgta atcggcttgc
     2341 cggggatagc cggcaccagc ttggcgacgc gatcgacgtc ggggagcggc atgtcgagca
     2401 cgcgacccac gtcacggatc gccgcgcgcg cgccgagcgt gccgaaggtg atgatctgcg
     2461 ccactttgtc gcgcccgtac ttgcgcacgg cgtattcgat catctcgccg cgccggtcgt
     2521 cggggaagtc cagatcgatg tcgggcatgc tgatgcggcc cggattgagg aaacgctcga
     2581 agatcaggcc gtgcgacagt gggtcgatca gcgtgatgcc cgccgtatat gcgacgatgc
     2641 tgccggccgc cgacccgcgc acgttccacc agatgccgct ctcgcgcgcg tagcgcgtga
     2701 ggtcgctgac gatcaagaag tacgtgtcga agcccatctg atgaatcacg ctcagctcgt
     2761 agtcgagccg ctggcgcact tcgggatcgt tcgcgcgctc gccgtagcgc tcggccagcc
     2821 cacgctcgca cacggcgcgc agatacgtct ccgccgtgtg gccttccggc acggggaagt
     2881 cgggcaagtg atagccctgg aagccgagat cgacctcgca catctcggcg acgagcagcg
     2941 tgttgctcag cgcttcggga atttcgccga acagcgccgc catctcgtct gcgctcttga
     3001 gatagtacga gtcgtcggtc attttcatgc ggtccggctc ggagacgagc gagccggtgc
     3061 cgatgcacag cagcacatcc tgcgcgtggg cgttctcggc gcgcaggaaa tgcacgtcgt
     3121 tggtggcgac cggccgcagc ccgaacgcct ggcccagcgc gagcagttgc cggttgacct
     3181 ggacgagatc ggggataccg tcgtgcaact gcaattcgac gaagaaccgc tcgcgcccga
     3241 acacgtcgac gtaccaggcc agcgcttcgc gggctgtgtc gatcgcgccg tcccggatca
     3301 agcgtggcac ttctgccgat ccgcatccgg tggtcaggat cagcccttcc gagaagcgct
     3361 ccagaacctc cttgtcgacg cggggacggt agtagaagcc ttccagttgc gagatcgagg
     3421 cgagctgcag caggttcttg tagccggtct cgttctgcgc cagcatgagc aggtgatgag
     3481 gcgatttgtc cagttgcgga tcgcggttgt gccgcgagcg gcgcgccagg tagccttcca
     3541 tgccgatgag gggcttgacg ccagcttgcc gagccgcatt gtagaaatcg atcgcgccgt
     3601 acatcacgcc gtgatcggtc agcgcgacgg cgggctggtt caattcctta gcgcgcttga
     3661 cgatgtgagc cggtttgttg aggccgtcga gaagtgagta ttcactgtga acatgcaggt
     3721 gcacaaaaga catcgcagcg gcgcccttcg ctgactgtgg agaaacaaaa agggtggaac
     3781 ggatattccg cacgtcatta taccacagcc ggatcgcgcg ggaatccccc ttccgctaca
     3841 ctctggctcg tctccccttc aggcccacgc aggttgatgg gttaacagac aagcccgtcc
     3901 tgacagcagg tggaatttgg aatgtatttg caatgtagtc tttgggcatc gggtggaacg
     3961 tttcggatat aatcatcctg cgattcttcg cagtcgggca actcaccgcc aacgggagag
     4021 gagctgtgat gcgctggcta tctcgccagt tgtgcgccgc cgttcgagcg ctcgcagtgg
     4081 tgttccttgt ggcctgc
//
LOCUS       DBNHXB010000084        11846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3370604, whole genome shotgun sequence.
ACCESSION   DBNHXB010000084 DBNHXB010000000
VERSION     DBNHXB010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11846
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3370604"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1730)
                     /locus_tag="AC162G_03205"
     CDS             complement(204..1730)
                     /locus_tag="AC162G_03205"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HIR5074856.1"
                     /translation="MSRRYLYLTVFTSGLVTLAVELSASRLIEPYFGTSNLIWATLIG
                     LILLYLSAGYALGGRWADRSPRADVLYRIIVWAAFLIGLIPFVAHPILSLAVTGFADF
                     NAALLAGSFIAVLILFSAPITLLGCVSPFAIRLAVDDVAHTGNVSGRVYAVSTVGSFF
                     GAFLPVLILIPAIGTRSTFVTLSLALMAAGLIGLRRRAIRFAWMPIAILLLAALVQPD
                     IKSTAGTIFQTESSYNYIQVVERGDTRYLLLNEGQGVHSVYNPNRVDTGGTWDFFALA
                     PYFNVPATADRLQRVAVVGLAAGTIPRQYSAIFGPVPIDGIEIDPRIVEAGRVYFDMS
                     LPNLNVVVADGRFALSRSPYRYDVVGVDAYRLPYIPWHLTTVEFFREVREHLTDAGVV
                     VINVGHAGNDYRLVDALTATLSQVFPSVYVADVPNTFNTIVYATAQPTSPDNLRANLA
                     SMTHSLARNAAERALPSLRAPSPGGAVFIDDRAPVEQLTNSIMVNYLLEIASGEITLP
                     "
     gene            complement(1767..3392)
                     /locus_tag="AC162G_03210"
     CDS             complement(1767..3392)
                     /locus_tag="AC162G_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HIR5074857.1"
                     /translation="MTDLSTHVVEVTVYPDRARVTRRGSAALEPGLHRLSIADLPLTL
                     DTASVRAGGKFRPSAASPPLASPPSDGGDGARLLGVDVRRTYYQDTPVATVKELEDQI
                     LALEDADRVLADQAEALAVQEAFARSVAEKAGEQLARGIAFSRTDVAQGSALMSFVAQ
                     EMTRTQNDRRELGIQRRDLAKQLEQLRNRLRMLQGSLGRERYTATVEVEVTAVGELTV
                     DLDYVVTSAGWLPLYDMRLDGGEVELAYLGQVTQRSGEDWNSVTLALSTARPALAAVL
                     PELKPWYVSVYEPPVVHPAAKAVMRVAAAPTAPAAVVPQPAAEFAVAEPVALEAVTAG
                     VESAGASVTFRLPQQATIPADGEPHKVTVATARLEPKLDYVTAPRLAEFAYRRAKAKN
                     GDLMLLPGAASLFVEGDFIGSAPLKLTAPGEEFETYFGVDDRIYVKRELKAREVDKKL
                     LQEKRRLHYGYEIEVRNLRPGRIDLEIHDQMPVSRHESVKVRLESVDPKHAEQSEMNE
                     LTWKLSLEPGAKQLVRFDFSVEHPRDLQVAGLP"
     gene            3631..3999
                     /locus_tag="AC162G_03215"
     CDS             3631..3999
                     /locus_tag="AC162G_03215"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HIR5074858.1"
                     /translation="MDTIAIIAHDGKKADMLAFASRNRDKLQRFHLLATQSTGRRLSD
                     ELGLDVELMLPGPLGGDAQIAARVVTGQIHAVFFFVDPLDKHPHEPDIQTLLRACNTH
                     NIPLATNVATADLIITARTV"
     gene            4031..5302
                     /locus_tag="AC162G_03220"
     CDS             4031..5302
                     /locus_tag="AC162G_03220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5074859.1"
                     /translation="MIHTNLRRNVAGILTDFVGFGTSLGFIGFDTLLPLLTFALTGNE
                     ALVGLVGTLWIGFWLLPQLAAGRWMAGRPRKKPVMIGTAAISRASLGLFVVMLALGES
                     LDRGLVFAGLVAMVAIFRGFDSVAAVAWFDIVSKSLPTDVRSRVFGAGQALANVFRFG
                     ASLVVTAAIYGGLVYPHSFAMLYGFAVIALALSLVGLLALREPVEKNGSSLSDQMGLL
                     AHAVHVLSKDKAFRQVTIVRLMVGLFDLARPQYIVHATNELGLPASNIGLFIAAQTIG
                     GILGSVALGRLCERRGSTAVIRISSLLAVSVPLIALALQAFGHGQPALATAGYLLVYV
                     LIGALDASFLLGFLVYVLDIAPPGERPAYTGLANTVAGVMVIAPTIGGIILQLTSYPV
                     LFLSAAMGGALALLGARRLPAAASSTEVVKA"
     gene            5299..6021
                     /locus_tag="AC162G_03225"
     CDS             5299..6021
                     /locus_tag="AC162G_03225"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR5074860.1"
                     /translation="MNRGRRIAWSALLVAGFLLVAAAWRWIEAGFQLLLDRDSVLAWL
                     TSFGVWAPVVFIGVSALQVVLAPIPGQFIGVAGGYLFGVWRGLLYSLIGTSLGAWAAM
                     WLGRRFGRPLVTRLAGEPALARFDRFADRRGPLFFFLVFLLPFVPDDLAFYAIGLSPL
                     PILPMLIMATVVRLPAALVSTLVGDNIARLPLELLILGLIGVVTAGVFIWKYQEKIEM
                     VLMRWIRRMALATSDRRDQDST"
     gene            complement(6039..7319)
                     /locus_tag="AC162G_03230"
     CDS             complement(6039..7319)
                     /locus_tag="AC162G_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR5074861.1"
                     /translation="MSRLRAALLLSAAMLIAGLATGRTMFYNVFFLLVAVILLSYLWA
                     WAGVNWLRLTRQTRSRRAQVGLPIEEVFRLRNIGSLPKLWLEVLDGSDLPGHRASHVA
                     YNIPPSTEYAWTVRTYCLERGRYRLGPITLVSNDPFGLFTIRRALSHTTNVVVYPATF
                     YLRSFPMPVGTLPGGDALRRRTHQITANAAGVREYYPGDSFNRIHWPSTARKNRLLVK
                     EFELDPMSDVWIFLDMAAASHYGERHVDVGQIVDRPSGQRPPKFELPNSTEEYCVSIA
                     ASIAQYFLRNNQSLGLVAAGQSWEIVQADRGDRQVLKILETLAVLRAEGKMPLYEILY
                     RETSGLARGSTIIIVTPSVNVRWARTARHLATTGLRVVPVLVDPQGFGGDAGAAQIAA
                     ELEVAGVKPVIIERGANWVDMFDRAQPVAGRWIP"
     gene            complement(7327..8283)
                     /locus_tag="AC162G_03235"
     CDS             complement(7327..8283)
                     /locus_tag="AC162G_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5074862.1"
                     /translation="MNSIQVVGRKLIDNIEKVIIGKHDTVTLAAIGLLCQGHLLIEDV
                     PGVGKTMLAKTIAKSIGCVFRRIQFTPDMLPSDVTGVRIYDQKTREFVFQPGPIMAQI
                     VLADEINRATPKTQSALLEAMEERQVTVDGETHYLRPPFMVIATQNPIEYEGTFPLPE
                     AQRDRFLLRIHIGYPDAKTEVAILDDQRTQHPIDTIDQVLSVDDLLAAQREVREVYVD
                     DLVKKYIVDLVNYTRRHDDVYLGASPRGSLTLYRGAQARAALDGRSYVIPDDVKYLSG
                     FALAHRMIVSPSARIKDVTADVVLREMLNSVPVPGGMVQPVR"
     gene            8509..8757
                     /locus_tag="AC162G_03240"
     CDS             8509..8757
                     /locus_tag="AC162G_03240"
                     /inference="COORDINATES: protein motif:HMM:NF019982.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5074863.1"
                     /translation="MIHTPTLVLAGGRDMMVPPSQGRHAARSISGARLVVWSDVGHQV
                     VDERGDDFDALLIDPVDRACGRRAGRTVGAGYPLQSEA"
     gene            8754..9338
                     /gene="thpR"
                     /locus_tag="AC162G_03245"
     CDS             8754..9338
                     /gene="thpR"
                     /locus_tag="AC162G_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIR5074864.1"
                     /translation="MNAVRAFIAVELPKVTRDALSEVQARLGQHAPRGSVRWVKPDNI
                     HLTLKFLGQVPTAQIEAISLALRRAVSVLHVFPFDVMRVGCFPDAQRPRVIWVGIDEP
                     SGALHALQRAVESATVPLGYPTEPRPFQPHLTLGRTGRDLRPADLHKLGEAVMAAKIG
                     LIGHVHADEIVLFQSELAPSGSIYTVLARFPLAH"
     gene            9413..10063
                     /locus_tag="AC162G_03250"
     CDS             9413..10063
                     /locus_tag="AC162G_03250"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015234496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR5074865.1"
                     /translation="MRFVDRVDAGRQLVDKLSSLKGESGLLVLGIPRGGVVVAYEVAL
                     ALGAPLDVALSRKLGAPQNEELAIGALAENGSRILDEVLIQHLRVPPDYVEHVTQQEM
                     KEIERRAAMYRGRRPPVDVRGRSVVVVDDGVATGATMIVTLEALRDQGVRKLIAAAPV
                     MSGEAIGRLRWIADEIVNLHVPSIFLAVGGYYQNFDQVSDAEVRELLADLAGTQSE"
     gene            complement(10156..11448)
                     /locus_tag="AC162G_03255"
     CDS             complement(10156..11448)
                     /locus_tag="AC162G_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013104.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074866.1"
                     /translation="MEPSLLPLSFLTPIGVLLTGWGTVPTSRLRESALAALVAAISSA
                     VAYIGFGFAFQFGGVGFSPTAPAGLTGLDTVWSPFPGSAGQWTFAGLEGFLLSAQGAP
                     ESLALLEPLALHRLSLAMAAGLIPVVALGNDANRVVTIAASIISSAIVFPLTGAWIWG
                     GGWLADLGTVLNLGHGAIDVAGSGIAFFGPACVAFVALRMFRQSGSSPDREVELPELR
                     QPLLATLGALLLGAGWCAWSMSDPLLSAYPTTEFGGVALIGLLCAAASMTAAGAYVWL
                     ASGRFHLGMFSRGWLAGWVAASASAWFIPLGSALVIGLISGIAMVFSQYIFERRWGLR
                     DRAGAVAIYGAAGAWGLIAAGIFADGAFGSGWNGITTPRGVQGILAADPGQLSAQLAA
                     LIAIGLFATCVAAALLLPIFFIVRRSASAPDAGVLPGE"
     gene            11453..>11846
                     /locus_tag="AC162G_03260"
     CDS             11453..>11846
                     /locus_tag="AC162G_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014037.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HIR5074867.1"
                     /translation="MDDTRPDSEDQKEPVRLIADVMLGRLAKWLRLIGYDTRYDNAAE
                     DVTLLRIAQQEDRILLTRDRGLSRQRGVRAIWIESQELAAQIRQVQAAVGPPPDGALT
                     RCAVCNARLEPIAKAGVEDRVPPYVFQTQ"
BASE COUNT         2091 a         3691 c         3933 g         2131 t
ORIGIN      
        1 ggcgacagtt gcactgtcgc ccagcgtccg cagtgcaatc cgggtcaaaa cgactaaagt
       61 cgttactacg tatgagcgac agttgcactg tcatccagcg tccgcagttg cactgcggac
      121 gaccaacttg tggataatca ccggctcctt gtgtgcctcc tgaccattag actactcgac
      181 tactcgacca tgcgaccact gcttcacggc agcgtgattt ctccgctcgc gatctccagc
      241 aggtaattca ccataatcga gttcgtcaat tgctcgaccg gcgcgcgatc gtcgatgaat
      301 acggcgccgc ccggcgacgg cgcgcgcagg ctgggcagcg cgcgctcggc ggcgttgcgc
      361 gccagtgaat gcgtcattga agccaggttg gcgcgcagat tatcgggaga cgtcggctgc
      421 gcggtcgcat acacgatggt gttgaacgtg ttcggcacgt cggcgacgta caccgaggga
      481 aagacctgcg acagcgtggc cgtcagcgca tcaaccaggc ggtagtcgtt tccggcgtgt
      541 ccgacgttga tcacgacgac gccggcgtcg gtgaggtgct cgcgcacttc cctgaaaaac
      601 tcgacggttg tcaggtgcca cgggatgtag ggcaggcggt aggcgtcgac gccgacgacg
      661 tcgtagcgat acggcgagcg ggatagcgcg aagcgcccgt cggcgacaac gacgttcagg
      721 ttcggcaggc tcatgtcgaa gtagacgcgg ccggcctcca cgatgcgcgg atcgatctcg
      781 atgccgtcga tcgggacggg gccgaaaatg gcgctgtact gtcgagggat cgtccctgcc
      841 gccaggccga cgaccgcgac gcgctgcagc cggtccgccg tcgccggcac gttgaagtac
      901 ggcgccagcg cgaaaaagtc ccacgtgccc ccggtgtcga cgcggttggg attgtagacg
      961 gaatgcactc cctggccttc gttgagcaga aggtaacgcg tgtcgccgcg ctccacgacc
     1021 tggatgtagt tgtaagagga ctcggtttgg aaaatcgtgc cggccgtcga cttgatatcg
     1081 ggctgcacga gcgcggccag gaggagaatc gcgatcggca tccacgcgaa gcgtatggcg
     1141 cggcggcgca ggccgatcag tccggcggcc atcagcgcca gcgacagcgt gacgaacgtg
     1201 ctgcgcgtgc cgatggccgg tatcaagatc aggaccggca ggaacgcgcc gaaaaagctg
     1261 ccgacggtgg acacggcata cacacggccc gacacgttgc cggtatgcgc cacgtcgtcg
     1321 acggccaggc ggatcgcgaa cggtgaaacg catccgagca gggtgatcgg cgcgctgaac
     1381 aggatcagca cggcaatgaa cgatccggcg agcagcgccg cgttgaagtc ggcgaagccg
     1441 gtcacggcca ggctcaggat cgggtgcgcg acgaacggga tcaacccgat gaggaacgcg
     1501 gcccagacaa tgatgcgata caacacgtcg gcgcgcggcg accgatcggc ccatcgcccg
     1561 ccgagcgcgt aaccggcgct gaggtacagc aggatgaggc cgatcagcgt ggcccagatc
     1621 aggttgctcg ttccgaagta aggctcgatc aggcgagagg cggataactc gacggcgagc
     1681 gtgaccagcc cgctggtgaa gacggtgagg taaagataac gtcggctcaa gcgtgagcgc
     1741 ctcaagtgtg tcgagaatga aaggtgttac ggcagcccgg ccacttgcag gtcgcgcgga
     1801 tgctccacgc tgaagtcgaa gcgcacgagc tgcttcgcgc caggctccag agaaagcttc
     1861 caggtcagct cgttcatctc gctctgttcg gcgtgcttcg gatcgaccga ttcgagcctg
     1921 accttgacgg actcgtgccg cgaaacgggc atctgatcgt gaatctccag gtcgattctt
     1981 ccggggcgca ggttgcgcac ttcgatctcg tagccgtagt gcagcctgcg cttctcctgc
     2041 aggagtttct tgtccacctc gcgcgccttc aattcgcgct tgacgtatat gcgatcgtca
     2101 acgccgaaat acgtttcgaa ctcttcaccg ggcgcagtca gcttgagcgg ggcgctgcca
     2161 atgaagtcgc cctccacgaa gagcgacgcc gcgccgggga gcagcatcag atcgccgttc
     2221 ttcgccttcg cccggcgata tgcgaactcg gccagcctgg gcgccgtcac gtagtccagc
     2281 ttgggctcga gcctggctgt ggcgacggtc accttgtgcg gctcgccgtc ggcggggata
     2341 gtggcctgct ggggcagccg gaaggtgacg gacgcgcccg cgctctcgac cccggctgtg
     2401 acggcttcca atgctaccgg ctcagccacc gcaaactctg cagcgggctg cggcacaact
     2461 gcggcaggcg ccgtgggcgc agcggccacc cgcatcacgg cttttgctgc ggggtgcacg
     2521 accggcggct cataaacgct cacgtaccag ggttttagct caggcagcac ggcggccagt
     2581 gcgggacgcg ccgtggacag ggcgagtgtc accgagttcc agtcttcgcc gctgcgctgc
     2641 gtgacctggc cgagataggc cagctcgacc tcgccgccgt cgaggcgcat gtcgtatagc
     2701 ggcagccagc cggcgctcgt cacgacgtag tccagatcga ccgtcagttc tcccacggcg
     2761 gtgacttcga cctcgacggt ggctgtgtag cgctcgcggc ccaggctgcc ttgcagcatc
     2821 ctgagtcgat tgcgaagctg ctccaactgc ttggccaggt cgcgccgctg gataccgagt
     2881 tcccggcgat cgttctgcgt gcgggtcatt tcctgggcga caaagctcat cagcgcgctg
     2941 ccctgcgcaa catcggtgcg cgaaaaagcg atgccccgcg ccagttgctc gccggccttc
     3001 tctgccaccg agcgggcgaa ggcctcctgc acggccaggg cttcggcctg gtcggcgaga
     3061 acgcgatcgg catcctccag cgccaggatc tggtcttcca gttctttgac cgtcgccacg
     3121 ggcgtgtcct ggtaatacgt ccggcggacg tcgacgccga gcaggcgcgc tccgtcccct
     3181 ccgtctgacg ggggggatgc cagggggggt gaggcggcgc tcggcctgaa cttgccaccc
     3241 gcgcgcaccg aggcggtgtc gagcgtgagc ggcaggtcgg caatgctcag ccggtgaagc
     3301 cccggctcca gcgccgccga accacgccgc gtcacgcgcg cacggtcggg gtagacggtc
     3361 acttcgacaa cgtgcgtgga cagatcggtc atgtcgattc tcccaatggc gatcggcgca
     3421 agtgctatta tagccgaatc gcgccataag gcatctgctc tgcgcctccc ctggcccttc
     3481 cgctgcgcgg cagcgcagag gcaggtgtgt gtctccgtgg cgagatctgt agcgtcacca
     3541 gacgtgaacg cctgcgccga gccgccgcag tcttgaccgc aatgtgcagg actgttatag
     3601 tcaggcggga tccactcccg tggagacgct atggacacga tcgccatcat cgcacacgac
     3661 ggcaagaaag cggatatgct cgcgtttgct tcgcggaacc gcgacaaatt gcagcgcttc
     3721 cacctgcttg ccacccagag caccggaagg cggttgagcg acgaactcgg cctcgacgtc
     3781 gaactgatgt tgccgggacc gctgggcggc gacgcgcaaa ttgcggcccg cgtcgtgacc
     3841 ggtcagattc acgccgtttt cttcttcgtc gatccgctcg acaagcatcc gcacgagcca
     3901 gacatccaga cgctgttgcg cgcctgtaac acgcataaca tccctctggc gacgaacgtt
     3961 gccacggcag acctgatcat cacggcccgc acggtctgat ggtgtcgcgg ctcaacgttc
     4021 gcccgctcat atgattcaca cgaacctccg ccgcaacgtc gcggggatat tgaccgattt
     4081 cgtcggcttc ggaacgtcgc tcggcttcat cgggttcgat acgctgctgc cactgctcac
     4141 gttcgcgctc accggcaatg aggcgctggt cggcctggtc ggcacgttat ggatcgggtt
     4201 ctggctgctg ccccagttgg ctgccggccg ctggatggcc ggccgtccac gcaagaagcc
     4261 ggtgatgatc ggcacggccg ccatctcgcg cgcttcgctc ggcctcttcg tggtcatgct
     4321 cgcgttaggc gagtcgctcg atcgcgggct ggtgttcgcc ggcctcgtcg cgatggtcgc
     4381 aatctttcgt ggcttcgatt cggtcgcggc ggttgcctgg ttcgacatcg tcagcaagtc
     4441 cctccccaca gacgtgcgca gccgcgtatt cggcgcgggc caggcgctgg caaatgtctt
     4501 ccggttcggc gcatcactcg tcgtcacggc ggctatctac ggcggactgg tatatcccca
     4561 ttcgttcgcg atgctatatg gcttcgctgt gattgcgctg gcgctctccc tggtaggtct
     4621 gctggccttg cgagagcctg tcgagaagaa cggctcgtcg ctgtccgatc agatgggcct
     4681 gctggcgcac gccgtgcacg tgctgagcaa ggacaaagcc tttcggcagg tgaccatcgt
     4741 gcggctgatg gtcggcctct tcgacctggc ccgtcctcaa tacatcgtgc atgccaccaa
     4801 tgagcttggc ctgcccgcct cgaacatcgg cctgttcatc gcggcgcaga cgatcggcgg
     4861 catcctcggc agcgtcgcgc tcggccggct gtgtgagcgc agaggctcga cggcagtcat
     4921 tcggatcagt tcgctcctgg ctgtgagtgt gccgctcatc gcgctcgcgc tgcaagcatt
     4981 cggccacggc cagccggctc tggcgacggc cgggtatctg ctcgtgtacg ttctgatcgg
     5041 cgcgctcgac gcatcttttc tgctcggctt cctggtgtat gtgctggata tcgctccgcc
     5101 gggagagcgg ccggcgtaca ccgggcttgc caacactgtc gccggtgtga tggtcatcgc
     5161 gccgaccatc ggtggcatca tcttgcagct cacgtcgtac ccggtgctat tcttgagcgc
     5221 ggcgatgggg ggcgcgttag cgctgctcgg cgcgcggcgc ttgcctgcag ctgcgtcatc
     5281 cacggaagtg gtcaaagcat gaatcgggga aggcgcatcg cgtggagcgc attgcttgtc
     5341 gccgggttcc tgctcgttgc agccgcgtgg cgttggatcg aggcgggctt ccagctcctg
     5401 ctcgatcgag atagcgtgct ggcctggttg accagtttcg gcgtctgggc gccggtcgtg
     5461 ttcatcggcg tgagcgccct gcaggttgtc ctcgcaccga ttcccggcca gttcatcggg
     5521 gtggcaggcg gatacctgtt cggcgtatgg aggggcttgt tgtacagcct gatcggtacc
     5581 tctctgggcg cctgggcagc gatgtggctg ggccgccgat tcggacgacc gctcgtgacg
     5641 cgactggccg gcgagccagc gctggcgcgc ttcgatcggt tcgcggaccg gcgcgggccg
     5701 ctattcttct tcctcgtctt cctactgcca ttcgttcccg acgatctcgc cttctacgcg
     5761 atcgggttaa gcccgctgcc gatcctgccc atgctgatta tggcgacggt tgtgcgcctg
     5821 ccggcggcgc tcgtatcaac gctcgttgga gataacatcg cgcgcctgcc gctggagctg
     5881 ctgatcctcg ggctgatcgg cgtcgtgaca gccggggttt tcatctggaa ataccaggag
     5941 aagatcgaga tggtcttgat gaggtggatc aggcgaatgg ccttggcgac gagcgacagg
     6001 cgcgaccagg acagtacata gcgcctggag attctaattt atgggatcca tcgtcctgcc
     6061 acaggttgcg cccggtcgaa catgtccacc caattggcgc cgcgctcaat gatgacgggc
     6121 ttgacgccgg cgacttcgag ctcggccgcg atctgcgcgg cgccggcgtc gccgccgaag
     6181 ccttggggat cgaccagcac ggggacgacg cggaggcccg tcgtcgctag gtgacgggcc
     6241 gtgcgcgccc agcgcacgtt gacggatggt gtgacgatga tgatagtcga tccccgcgcc
     6301 aggccgctcg tctcgcggta gaggatctcg tataacggca ttttgccctc ggcgcgcaga
     6361 acggccagcg tctccagaat tttcaacacc tgccgatcgc cacggtcggc ctgcacgatc
     6421 tcccagcttt gtcctgcagc gaccaatccc agcgactgat tgttgcggag gaaatactgc
     6481 gcgatcgagg cggcgatgga gacacagtac tcctccgtgc tgttgggtaa ctcgaatttg
     6541 ggagggcgct gtccactggg ccgatcgacg atctgaccca catcgacgtg gcgctcgccg
     6601 taatgggagg cagccgccat gtccaggaag atccacacgt cggacatggg atcgagctcg
     6661 aactctttca ccaggaggcg gttcttgcgt gctgtcgagg gccagtgaat gcggttgaag
     6721 ctgtcgcctg gataatactc acgcacaccg gcggcattgg ccgtaatctg gtgtgtgcgg
     6781 cggcgcagcg catcgccacc agggagcgtg ccgaccggca tgggaaaaga tcgcaggtag
     6841 aaagtcgccg gatacacgac gacgttcgtc gtgtggctga gtgcgcgccg gatcgtgaac
     6901 aggccgaaag gatcgttgct gacaagcgtg atcgggccaa ggcgatagcg gccgcgttcg
     6961 aggcagtacg tgcggaccgt ccaggcgtac tccgtggacg gcgggatgtt gtaggccacg
     7021 tggctggcgc ggtgaccggg cagatcggaa ccgtccagga cctccagcca cagcttcggc
     7081 agcgagccga tgttccgcag ccggaacact tcttcaatcg gcagcccgac ctgcgcccgg
     7141 cgcgagcggg tctggcgggt gaggcgcagc cagttgacgc cggcccaggc ccacaaatag
     7201 gacaggagga tgacggcgac cagcaggaaa aagacgttat agaacatcgt ccggccggtc
     7261 gccaggcctg cgatcagcat ggcagcgcta agcaagagtg cggcgcgcag acgggacatg
     7321 ggtgacctat cgcaccggct gcaccatgcc gcccggcacc ggcacgctgt tgagcatctc
     7381 gcgcagaacg acgtcggcgg ttacgtcttt gatgcgagcc gaggggctga cgatcatgcg
     7441 atgggccagc gcgaacccgg agagatattt gacatcgtcc gggatcacgt agctccggcc
     7501 gtccagggcc gcgcgcgcct gcgcgccacg gtacagcgtc agcgaaccgc gcgggctggc
     7561 gccgaggtat acgtcgtcgt ggcgccgcgt atagttgacc aaatcgacga tgtacttctt
     7621 gaccagatcg tcgacgtaga cctcgcgcac ctctcgctgg gcagccagta agtcatcgac
     7681 gctcagcacc tggtcgatcg tgtcgatcgg gtgctgtgtg cgctgatcgt cgaggatggc
     7741 aacctcggtc ttggcgtccg ggtagccgat gtggatgcgg agcaagaagc gatcgcgctg
     7801 cgcctcggga agcggaaaag tgccttcgta ttcgataggg ttttgtgtgg cgatgaccat
     7861 gaacggtggg cggaggtagt gcgtttcgcc gtcgaccgtg acctggcgct cctccatcgc
     7921 ttcgagcagc gccgactgcg tcttgggcgt ggcgcggttg atctcgtcgg ccagaacgat
     7981 ttgagccatg atcggcccgg gctgaaagac gaactcgcgt gtcttctggt cgtaaatgcg
     8041 cacaccggtc acgtcgctcg gcagcatatc cggtgtgaac tgaatccgcc ggaagacgca
     8101 tccgatcgac ttggcaatcg tcttggctag catcgtctta cccacgccag gcacatcctc
     8161 gatcaacaag tgtccctggc ataacagccc gatggcggcc agcgtgaccg tatcgtgctt
     8221 cccgatgatg accttttcga tgttgtcgat cagttttcgg ccgacgactt gaatgctatt
     8281 cattccgatg tccttgggtg tgatgggaat ccggagcgcg cgccgatctt acatgcgcct
     8341 gccaaccgtg tcttcgcggt tggcaagcac ctccggcaac tgcgtagtat acactgaaga
     8401 tacataaagg gttcagcggc gtcagggtgg ggcgcatggc ggcccaatta gattaccaca
     8461 aatatgcgga tgaaaaaaac cgatgcggct atgctataat atcaaataat gattcacact
     8521 ccgaccctcg tgctcgccgg cggtcgagat atgatggtgc cgccatcgca ggggcgccat
     8581 gcggcgcgct cgatttcggg ggcgcggctg gtggtgtggt cggacgtggg ccaccaggtc
     8641 gtcgacgagc gcggcgatga cttcgacgcg ctcctgatcg accctgtcga tcgggcctgc
     8701 gggcgccgcg ccggacgaac ggtcggcgcg ggatatcctc ttcagtcgga agcatgaacg
     8761 cggtacgggc cttcattgcc gtggaactgc cgaaggtcac gcgagatgcc ttgtcggagg
     8821 tgcaggcgcg tctgggccag cacgcgccgc gcggcagcgt gcgctgggtg aaaccggaca
     8881 atatccacct cacgctgaag ttcctgggcc aggtccccac tgcccaaatc gaggctatca
     8941 gcctggcgct caggcgcgcg gtgagcgtgc ttcacgtgtt ccccttcgac gtcatgcgcg
     9001 tgggctgctt tccggacgcg cagcgccccc gtgtcatctg ggttggcatc gacgagccga
     9061 gcggggcgct acacgcgttg cagcgcgccg tcgagtcggc gaccgtgccg ctggggtacc
     9121 cgactgaacc gcgtcccttt cagccgcacc tcacgcttgg gcgtaccggg cgggacctca
     9181 ggccagccga tctacacaag cttggcgaag ccgtgatggc ggcgaaaatc ggcctgatag
     9241 ggcatgttca tgccgacgag atcgtgctct tccaaagtga gcttgcgcca agtggatcga
     9301 tctacacggt acttgcgcga tttcccctgg cgcactagct gcgccacccg atcgctatgg
     9361 gtcttcacgt tccgcatagt ttacgctgag tgagggtcag ttcgagggcg gtatgcgatt
     9421 cgtcgatcgc gtcgacgccg gacggcaatt ggtggacaag ctgagcagcc tgaagggcga
     9481 aagcggcttg ctggtgctgg gcatcccgcg cggcggcgtg gtggtcgctt atgaagtcgc
     9541 gctggcgctt ggcgcgccgc tcgacgtggc gctctcgcgc aagctcggcg cgccgcaaaa
     9601 cgaagagttg gccatcgggg cgcttgccga gaacggcagc cgcattctcg acgaagtcct
     9661 gatccagcac ctgcgcgtgc ctcccgacta cgtcgaacac gtcacgcagc aagagatgaa
     9721 ggaaatcgag cgccgcgctg caatgtatcg cggccggcgc ccgcccgtcg acgtgcgcgg
     9781 acgcagcgtc gtcgtcgtcg acgacggcgt cgccaccggc gcgacgatga tcgtgacgct
     9841 ggaagcgctg cgcgatcaag gtgtgcgcaa gctgatcgca gccgcgcctg tcatgtcggg
     9901 agaggcgatc ggccggctgc gctggatcgc ggatgagatt gtgaacctgc acgtcccgag
     9961 cattttcttg gcagtcggcg gctattacca aaatttcgat caagtgagcg acgcagaggt
    10021 gcgggagctg ttggcggacc tggcaggcac gcaatctgag tagatccgat ttctgcggag
    10081 cgggcacgcc tacgtgctgg ccttggccgt acccttcgac actcccttcg gtcgcaggac
    10141 aggctccgag ggtgcttact ctcctggcag aactccggcg tctggggcgg aagcggatcg
    10201 ccgcacgatg aagaaaatgg gtaggagtag cgctgcggcc acgcacgtgg cgaacaggcc
    10261 gattgctatc agcgcggcca gttgggcgct gagctgtccc ggatccgcgg ccaggatacc
    10321 ctgtactccg cgtggcgtcg tgataccgtt ccagcccgag ccgaaagcgc catcggcgaa
    10381 tattccagcg gcgatcagcc cccaggcgcc cgccgcgcca taaatcgcca cggccccggc
    10441 gcggtcccgc agcccccatc gacgctcgaa gatgtactgg ctgaatacca tcgcgattcc
    10501 agagatcagg ccgatgacga gcgcgcttcc cagcgggatg aaccaggctg aagcgctggc
    10561 ggctacccac ccggccagcc agccgcgtga aaacattccc aggtggaagc ggccgctggc
    10621 gagccagaca tatgcgcctg cagcggtcat cgaggcagcc gcgcagagaa gaccgatcag
    10681 cgccacgccg ccgaattcgg ttgtggggta tgcgctcaga agaggatcgc tcatcgacca
    10741 cgcgcaccag cctgcgccaa gcaggagagc gccgagcgtc gccagaagcg gctggcgcag
    10801 ctctggcaat tcgacttccc gatccggcga tgagcctgac tgccgaaaca tgcgaagtgc
    10861 gacgaaggcg acgcaggccg gcccgaagaa agcgatccca gaaccggcca cgtcgattgc
    10921 accgtggccg aggttgagga ctgtgcccag gtctgccagc caaccgccgc cccatatcca
    10981 cgcgccagtg agtgggaaca caattgcgga cgagatgata ctggcggcga tcgtgacgac
    11041 gcggttcgcg tcattgccaa gcgccacgac tggaatcagt ccggcagcca ttgcgagcga
    11101 taggcgatgc agcgccagcg gttccaggag tgccaggctc tcaggcgcgc cctgggcgct
    11161 cagcaagaag ccttccagcc cggcgaacgt ccactggccg gcgctgccgg gaaatggcga
    11221 ccagaccgtg tcgagtcctg tcagcccggc cggcgcggtt gggctgaagc ccacgccgcc
    11281 gaactggaat gcgaatccga atccgatgta agccacggca ctgctgatcg ccgcgacgag
    11341 cgcggcgagc gccgactcgc gcagccgaga ggtcggcacg gttccccagc cggtgagcag
    11401 tactccgatg ggggtgagaa aggaaagcgg cagtagcgaa ggctccacgg cgatggatga
    11461 taccagaccc gattcagaag atcaaaagga acccgtaagg ctgattgccg acgtcatgtt
    11521 agggcggctg gcaaagtggc tgcggctgat aggctacgat acgcgctacg acaatgccgc
    11581 cgaagacgtg accttgctgc ggatcgcaca gcaagaggac cgcatcctgc tcacgcgcga
    11641 tcgggggttg tcgcgccagc ggggcgtgcg ggcgatctgg atcgaatcgc aggagctggc
    11701 tgcgcagatc cgccaggtgc aggcggccgt cggtccaccg ccggatggcg cgctcacccg
    11761 gtgcgcggtg tgcaacgcga ggctcgaacc gatagccaag gccggcgtcg aagaccgtgt
    11821 gccgccgtac gtgttccaga cacaag
//
LOCUS       DBNHXB010000085         2304 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2699677, whole genome shotgun sequence.
ACCESSION   DBNHXB010000085 DBNHXB010000000
VERSION     DBNHXB010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2699677"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="AC162G_03265"
     CDS             <1..1315
                     /locus_tag="AC162G_03265"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004467 - long-chain fatty acid-CoA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HIR5074868.1"
                     /translation="LVTLTRFGNLGKAIQASSSVEHVIFANVKDYLPPLRRAAFSLAR
                     EDREGHRLAFDLEPGMHRWNELLWRYPPVRPGVEPEPDDVALIIYTGGTTDRPKGVML
                     SHRNLVANAIQTRHWISDAREGRERFLCVLPFSHIYGLTTALNVPVMLGATMIILPTF
                     QTREVLQTIRATRPTMFTGVPTMYMALNNDPGVRRYGIRSIRACISGAAPLPVEVQEA
                     FEKLTRGRLVEGYGLTEASPVTHGNPLNGLRKVGSIGVPFPNTDAKIVDLATGEDLPP
                     GQIGELAVRGPQVMLGYWGMGDESDRVLRDGWLYTGDIAVKDEDGYFTIINRKKEMIL
                     AGEYQVYPRDVEEVLFEHPKVKDVAVVGVPATPPGQKVKAFVVLREGESATPDELIAL
                     CRQRLDAWAVPWEVEFRRELPKSFVGKVLRRVLVEEEESGGDQQG"
     gene            1299..1787
                     /gene="greA"
                     /locus_tag="AC162G_03270"
     CDS             1299..1787
                     /gene="greA"
                     /locus_tag="AC162G_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR5074869.1"
                     /translation="MTNKGNANGEPNYLTPDGKRRLEEELEYLSTTKRKELADRLFVA
                     TRQGDLAENADYIHAKEEQAFLEGRIKTIQALLRNVVLIEEVGGGGIVRIGSKITVSE
                     DGLDELETFLMVGAAEADPTKGRISNVSPLGQALMGKMVGDRVTIQAPAGEIVFRIVA
                     IE"
     gene            1920..2249
                     /locus_tag="AC162G_03275"
     CDS             1920..2249
                     /locus_tag="AC162G_03275"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR5074870.1"
                     /translation="MTSEQMRKSIQDIVEKLVTDYAPQKVILFGSYAYGDPRPDSDID
                     LLIIKETPERFIDRWVTVRRILSDPKRTFPLETVVLTPQEVSKRRAVGDQFVEEILEK
                     GIVLYAA"
BASE COUNT          461 a          661 c          793 g          389 t
ORIGIN      
        1 tctggtcaca ctgacgcgct tcgggaatct gggcaaggcg atccaggcga gcagcagtgt
       61 agagcacgtg atctttgcga acgtcaagga ttatctgccg ccgctgcgcc gggctgcgtt
      121 ttcgctggcg cgggaagaca gggaagggca tcgactcgcg ttcgatctcg agccgggcat
      181 gcatcggtgg aatgagctgc tgtggcgcta cccgccggtg agacccggcg tcgagcctga
      241 gccggacgac gtggcgctca tcatctacac cggcggcacg accgatcggc ccaagggtgt
      301 catgctgtcg caccgcaacc tggtggcgaa cgcgatccag acgcgccact ggatcagcga
      361 cgcgcgcgag ggacgcgagc ggttcttgtg tgtgctgccc ttctcgcaca tctacggcct
      421 gacgactgcg cttaacgtgc cggtcatgct cggcgcgacg atgatcatcc tcccgacgtt
      481 ccagacgcgc gaggtgctgc aaacgatcag ggcgacgcgc ccgacgatgt tcaccggcgt
      541 gccgacgatg tacatggcgc tcaacaacga ccccggcgtg cgccgctacg gcatccgctc
      601 gatccgggcc tgcatcagcg gcgcggcgcc gctgccggtc gaggtgcagg aggccttcga
      661 gaagctcacg cgcgggcgcc tggtggaagg ctatggcctg acggaagcgt cgccggtgac
      721 gcacggcaac ccgctcaacg ggctgcgcaa ggtcggctcg atcggcgtcc cgtttccgaa
      781 caccgacgcg aagatcgtcg atctcgcgac cggcgaagac ctgccgccgg ggcagatcgg
      841 cgaactggcc gtgcgtggcc cgcaggtgat gctcggctac tggggcatgg gcgacgagtc
      901 tgaccgcgtg ctgcgcgacg gctggctgta caccggcgac atcgcggtga aagacgaaga
      961 cggttacttc acgatcatca accgcaagaa ggagatgatc ctggccggcg agtatcaggt
     1021 ctacccgcgc gatgtcgaag aggtcttatt cgagcatccg aaagtgaagg acgtggcggt
     1081 cgtcggcgtg ccggcgacgc cgcccggcca gaaggtcaag gccttcgtcg tgctgcgcga
     1141 gggcgagtcg gccacgccgg acgagctgat cgcgctgtgt cgccaacggc tggacgcgtg
     1201 ggccgtgccg tgggaagtcg agtttcggcg cgagctgccg aagagcttcg tcggcaaagt
     1261 cctgcgccgt gtgctcgtcg aggaggagga gagtggtggt gaccaacaag ggtaacgcga
     1321 acggtgagcc gaactacctg acgccggacg gcaagcgccg gctggaagaa gagctggaat
     1381 acctgtcgac gacaaagcgt aaggaactcg ccgaccggct gttcgtggcg acccggcaag
     1441 gcgacctggc ggagaacgcc gactacattc acgccaaaga ggagcaggcg tttctggaag
     1501 gccgcatcaa gacgatccag gccctgctgc gcaacgtcgt gctgattgaa gaggttggcg
     1561 gcggcggcat cgtgcgcatc gggtcgaaga tcaccgtcag cgaagacggg ctggacgagc
     1621 tggagacgtt cctgatggtc ggcgcggcgg aagccgatcc gacgaaaggc cgcatcagca
     1681 acgtgtcgcc gctggggcag gcgctgatgg gcaagatggt cggcgaccgg gtgacaattc
     1741 aggccccggc cggcgagatc gtgtttagga ttgtggcgat tgagtgatga atcagcgagc
     1801 cagcgagttc gcctgtgctc agatgtggag tcgagccttc aggcggatcc ggctgacgct
     1861 tcgactccat gcagcgagat ggcgtccaag attgtggcga ctgagcagga aaggatctga
     1921 tgacttcaga acagatgcgg aagtcaattc aagacatcgt agagaaactc gttacagact
     1981 acgctccaca gaaagtgatt ctgttcggct cgtatgccta cggcgatcca cggccggaca
     2041 gtgacattga cctactgatc atcaaagaaa cgcccgagcg attcattgac aggtgggtga
     2101 cggtgcgccg cattttgtcc gacccgaagc ggacgttccc tctggagacc gtggtgctta
     2161 ccccccaaga agtgtcaaaa cggcgggcag tcggcgatca gtttgtggag gagatcctgg
     2221 agaaaggaat agttttatat gccgcgtgaa gagtcgctct atcccgccga ttggctacgc
     2281 attgctgaaa aagaccttgg ccga
//
LOCUS       DBNHXB010000086        10461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3706448, whole genome shotgun sequence.
ACCESSION   DBNHXB010000086 DBNHXB010000000
VERSION     DBNHXB010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10461
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3706448"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..756)
                     /locus_tag="AC162G_03280"
     CDS             complement(73..756)
                     /locus_tag="AC162G_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR5074871.1"
                     /translation="MPSVWIVTKTNPALVEAFQQAEWEVEVFAPPDLVPASQSRVSGL
                     DVIIFEVTEAAGPFFDKFKELCQDKLAPILAVVPNWAFADLVSAAGADAVIVMPVNLS
                     ELLWRARDLVRAKNVVHVGELAIDLAAMTVKRGGHLIHLPPLEYRLLASLAERIGQAV
                     SHDDILLYVWQYHPKWGGTLEQVKTCVKRLRQAIEPDPHHPQYIITIRRVGYQLRNQA
                     QWQAATRSS"
     gene            complement(1049..2878)
                     /locus_tag="AC162G_03285"
     CDS             complement(1049..2878)
                     /locus_tag="AC162G_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR5074872.1"
                     /translation="MSLDRLTIGRAASVPENDREPRRDLGARNPFAFVRHSIASWRGF
                     RRPERGVLEGALALARRGNGQTVFVATRGLHSFADRRLDMIEAKAIGPVRPYGLIRDL
                     LNAALPRSDARATGATLPADLLAYLKNPASLTGEPDGVTPALSEVEGKVEGWTDAVVD
                     FFAALPARAPMTILVRHAHHADPESVRVLEALARRVATSRILLLVTYRIDAPINAPLE
                     TTMRALLDAHLARAMALAAPQTDDARLLFLSAFGRSVRSEAAAASMAEGEAELLTYGM
                     LGVEACRRAGRWAEAKALAERVARAGESHQADYIACSAAIALGHILADQGKWDDSRER
                     LEPIQATVEATKEDDLIAWLYWGLARVYWGLSQRRRAFEILRLAHTVSVRTSDTSLAA
                     AIAVCGAEWLADNRRVSASQDWLESLEQIAGRADSPPAQAALALGSGVVALAEHDAIT
                     ATGFFRAALRRYTELGDDYASARARTRLAAALLEQDAADSRREGREELSEAHAAFTHL
                     SAADDVSAVEALGAKHGVRPRARRAPSGNSTSPGGITPREREVLELLVRGLTNRQIAA
                     TLSITEKTAEGHVSNILAKLGVASRGQAAGYAIANGLLEPAEA"
     gene            complement(3260..5941)
                     /locus_tag="AC162G_03290"
     CDS             complement(3260..5941)
                     /locus_tag="AC162G_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5074873.1"
                     /translation="MFEFIGKKLGAYEIGEPIGQGGMATVYKAFQPGMNRTVAVKVLP
                     PLLAQNATFLARFRQEAQVIARLEHAHILPVYDYGESDGVVYIVMRYLDGGSLQKKID
                     AGRMPLREVSKVLSQIASALDYAHRQGVIHRDIKPSNVLLDRQGDAFLTDFGIAKMVA
                     GTLDLTGSSVIGTPQYMSPEQGQGFEIDGRSDVYSLGVMLYEALAGCVPFDAETPLAV
                     VLKHVTEPLPSARAHAPDLPESVERVLDTALAKKPGDRYATAGELAAAFDDAIRTGLN
                     PATQPRVARQAPTQPAAPLRPTIVTRKPLRLPRPALAGVLVAVVFFALIGLLAVVVGS
                     LPPPDSPSGGVTFEPNILPLSNGTPTAPPLSLPPSERGEPEGGQAAAAPAACEPIVFD
                     DFSDPGGGFPRGENNHAAWGYVGGEYQMLIKLANELEARALRQDFADYAVEVDAYLAS
                     EAPGHYGLVLGADAAVNTYTAFVVDSAQNFAVMRRDPDGARSARDWTFAPSLHTGNVV
                     NRLRAVHKGDTLALYGNGVLLAVVGDAPGTSSSGSRIGLTAASFEKGGVDARFDNFRV
                     CPAPESLSPTEVSLVDAFDDNRNLWGAVRYPGGLAAFIEDGQFQLSVPSLEPRQALLQ
                     WNPMFATGDFELEVDSRALEGSGGSRAGVMFGVQDLDNNYIVYASADGLLSFYKRIEG
                     EQQAIVLDRPTEAIRTGDAVNHWKIIVAGDLLSVWLNGVQVLQASIAYTPGAIGFACE
                     PDASRPPLARCAFDNLSVHGQPSTGDVVVSPLCNCTREVRQGQPVRVVWLWKASEGPL
                     VDTFLSSISMSVTVDGKPLEDPGQYWGPVESIEEGAQARWSYSMPQLEPGSHIVEVGA
                     SADTELTDGRDANADGRPDTFGPGPLFTGFVQVIVEP"
     gene            complement(6008..7171)
                     /locus_tag="AC162G_03295"
     CDS             complement(6008..7171)
                     /locus_tag="AC162G_03295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5074874.1"
                     /translation="MRIGIDVTSAVTQSAGIGRYTRELVRALFALGAPHHYTLFYATE
                     KKVKQPIAPLPAGVRLRRLPIHDKWLARVWHRFRLPIPVELATGRLDLFHSPDFTLPP
                     VLPGTRTLLTVHDLSFIRDPDSAVPSLRRFLDRAVRRSVARAGRVLADSQATKDDLVE
                     LFGVPAGKIDVLLSGVDARFRPIRDPAALAAVRAKYNLGHGRFILSVGTIQPRKNYVR
                     LIRAFGQVVGRWWQIGSDLLGDVNLVIAGDKGWMFDDIFSEVTRLGLEGRVKFTGFID
                     EPDLPALYSAATLFAYVSLYEGFGLPVLEAMACGAPVIGSRTSSIPEVVGDAGLLVDP
                     TDVDAIAAGLIGLLKDPSMRDAYMRDGLRRASKFTWDAAARQLLSIYGEMAAR"
     gene            complement(7281..7796)
                     /locus_tag="AC162G_03300"
     CDS             complement(7281..7796)
                     /locus_tag="AC162G_03300"
                     /inference="COORDINATES: protein motif:HMM:NF016060.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR5074875.1"
                     /translation="MAASDALSTAARRESKRFAKFLVVGAIGAVVDFGAFNLLFRVFH
                     VDARLAQAISFTLAVTSNFTWNRYWTYPESRSKPLSRQYAQFFILNLIALLVRTLIFD
                     ALDEPLIALAAASLDGPLGFIARFAVDTLNMTLEQLGNNLALACAVVTMMLWNFFANR
                     FITYGDVKFGH"
     gene            complement(7796..8332)
                     /locus_tag="AC162G_03305"
     CDS             complement(7796..8332)
                     /locus_tag="AC162G_03305"
                     /inference="COORDINATES: protein motif:HMM:NF016060.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR5074876.1"
                     /translation="MSTPPVERKSIRAQVADFHTPLRIRERVRRRVPPAHRKEAKRFM
                     KFAIVGSIGFVIDTGALTLLVFLFGLTGDWQRLIAKAISFTLAVISNFLWNRYWTYPE
                     SRSKPIRRQMTQFFLLNLMGLGINLIVFGFVDSLLTPIVGPALALYGAQVSAVTVAML
                     WNFAANRLITYNDVKFGW"
     gene            complement(8329..9885)
                     /locus_tag="AC162G_03310"
     CDS             complement(8329..9885)
                     /locus_tag="AC162G_03310"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR5074877.1"
                     /translation="MSRITVHSSRYHVSRYHVSRFTLPLLAVLGGVLIARLPLSAAVV
                     ALGVGSVVAGALIEPALGLIALLVVAPWAAWQNTYLPGLLPTDAGQLTVALVLGAWVG
                     RGLIRRELDIPRMALLIPLSIFTGWAAITMLWAPDYSFGLPEVLKWIELILIALFAVD
                     VVRRRGAKWIVAGLLLSAAVQALVGIYEARIRGVGPEGFLLSEGVYRAYGSFEQPNPF
                     AGFIGLNLPIAIALAPALWGSAWQARATYDVPGFRQRILRFTFHASLVSFTVLLLATA
                     LYLSFSRGAWLGAAAALLAMGLFLPRRRWIGLAVIGGLVVIGLGLYGANLLPASIADR
                     FADVGALVDFRDVRGVKINDANYAVVERLAHWQAAVRMIETHPWSGVGFSNYQPVYEQ
                     YRLLNWPMPLGHAHNIYLNVAAEIGLPGLLAYLGLWTAIFALTIRTLDRQSGPYRWLA
                     LGLLGAWVHLSVHHLFDNLYVNNVHLHIGAMIGLLSAIGAAALTPGPSPPAHSRRGQA
                     VADDRRGGAG"
     gene            complement(9935..>10461)
                     /locus_tag="AC162G_03315"
     CDS             complement(9935..>10461)
                     /locus_tag="AC162G_03315"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR5074878.1"
                     /translation="PIGPRGVYDEAKRFAEAMTMAYHRYHGVDTRIVRIFNTQGPRMR
                     LADGRVVPNFVAQALRGEPLTIYGDGKQTRSFCYVDDLIEGIYRLLFSDEHEPVNLGN
                     PTELSIIEFAEVINRLTGSTAGVVYQPEKRIQDDPQTRQPDISKAKQLLGWEPKVPLE
                     EGLRRTIEHFRTRV"
BASE COUNT         1920 a         3372 c         3442 g         1727 t
ORIGIN      
        1 atatcaagaa tcctcgcgcg atgggtgacc gtgggtggca atttgggcgc tcaaagtggc
       61 agttttgtca ctttaggacg aacgtgtcgc ggcttgccac tgtgcctgat tcctcagttg
      121 gtagcccact cgcctgatgg tgattatgta ttgtggatgg tgtggatcag gctcaatcgc
      181 ctgccgcagc cgctttacgc aagtcttgac ctgctccagc gtcccgcccc acttggggtg
      241 atactgccac acgtacaata gaatgtcgtc gtgactgaca gcctgcccga tacgctccgc
      301 cagggaggcc agcagccggt actccagcgg cggcaggtgg ataaggtgac caccgcgttt
      361 cacggtcata gcagccagat cgatcgccag ctcgccgacg tgaacgacat tcttcgcgcg
      421 aaccaaatcg cgcgcgcgcc ataacagttc actgagattg actggcatga caattacggc
      481 atctgctccg gctgcggaca ccagatcggc gaaggcccag ttcggtacga cagccaggat
      541 cggggcgagt ttgtcttggc aaagctcctt gaacttgtcg aagaacggcc ccgccgcctc
      601 cgtcacctcg aagatgatta catcgagacc cgataccctc gattggctcg cgggcacgag
      661 atcgggaggc gcaaagacct cgacctccca ctcggcctgc tggaaggctt cgaccagtgc
      721 cggattcgtt ttggttacaa tccaaacact cggcatgaga cacgttcacc cacacgcgtt
      781 cggtggtggt caccaacccc gccggccctt ttggacgata gtcttttcgt tgtaataaac
      841 gctattttcg gcggatttcc atcggcatca ccttgcttca gacgcgcccg aagcccgaaa
      901 atgggccatt ttccctggaa aatatgggca caaccggaag ttcataacat gctctagagg
      961 gtcgcgcttc aacaaaaagc gccccgaagt aaaccttcag ggcgcagata gacactcctg
     1021 tttgttaaac tgcttcagag gctctgtgtc acgcctcagc cggctccagc agtccgtttg
     1081 caatagcgta tccagccgcc tgccctcgcg aggctacccc cagcttcgcc aggatgttgc
     1141 tgacgtgccc ctccgccgtc ttttccgtga tcgacagcgt cgccgcgatc tgccggttgg
     1201 tcaggccgcg cacgagcagc tccagcactt cgcgctcgcg cggggtgatg ccgcccggcg
     1261 aggtcgagtt gccggatggc gcgcggcggg cgcgcggacg aacgccgtgc ttcgcgccga
     1321 gcgcttcgac ggcgctcacg tcgtcggcgg cgctcaagtg cgtgaaagcc gcgtgcgcct
     1381 cgctcagctc ttcgcgccct tcgcggcggc tgtcggcagc atcctgctcg agcagcgcgg
     1441 cggccaaccg tgtccgggcg cgggccgagg catagtcgtc gcccagctcg gtatagcgac
     1501 gcagcgccgc gcggaagaag ccggtcgcgg tgatggcatc gtgctcggcc agcgcgacga
     1561 cgccgctgcc cagcgcgagc gccgcctggg ccggcgggct atctgcgcgc ccggcaatct
     1621 gctcgagcga ttcgagccag tcctgcgacg cgctcacgcg ccggttgtcg gccagccatt
     1681 cggcgccgca caccgcgatc gcggcggcga gcgacgtgtc ggacgtgcgc acactcaccg
     1741 tgtgcgccag gcgcagtatc tcgaacgcgc gccgccgctg agacaggccc cagtacacgc
     1801 gcgccaggcc ccaatacagc cacgcgatca gatcgtcctc tttggtcgcc tcgaccgtcg
     1861 cctggatagg ctcgagccgt tcgcggctgt cgtcccactt gccctggtcg gccaggatat
     1921 gcccgagtgc gatcgcggcc gagcatgcga tgtagtcggc ctgatgcgat tcgccggccc
     1981 gggcgacgcg ctcggcgagc gccttggcct ccgcccagcg cccggcgcgc cggcaggctt
     2041 cgacgccgag catcccatat gtgagcaatt ccgcttcacc ctcggccatc gacgctgcgg
     2101 cggcctcgga ccgcacggag cgtccgaaag cggacaggaa cagcaggcgc gcatcgtctg
     2161 tctgcggcgc ggcgagcgcc atggctcggg ccaggtgcgc gtcgagcagt gcgcgcatcg
     2221 tcgtttcgag cggcgcgttg atcggcgcgt cgatgcggta cgtgacgaga agcagaattc
     2281 gcgacgtggc gacgcggcgc gcgagcgcct cgagcacgcg cacgctctcc ggatcggcgt
     2341 ggtgcgcgtg ccgaacgagg atcgtcattg gcgcgcgcgc aggcagcgct gcaaagaagt
     2401 cgaccacggc atccgtccac ccttcgactt tcccttcgac ttcgctcagg gcaggcgtca
     2461 cgccgtcagg ctcgcctgta agagaggctg gattcttgag gtaggccagc agatcggcag
     2521 ggagcgtcgc gccggtggct cgcgcatcgg aacggggcag cgccgcattg aggaggtcgc
     2581 ggatcagtcc atacgggcgc accggcccga tcgccttcgc ttcgatcatg tccaggcgcc
     2641 ggtcggcgaa cgagtgcaag ccccgcgtgg cgacgaacac ggtctggccg ttcccccggc
     2701 gcgccagggc gagcgcgccc tcgagcacgc cgcgctccgg tctgcgaaag cctcgccacg
     2761 aggcgatgct gtgccggacg aacgcgaagg ggttccgcgc gcccaggtcc cggcgaggct
     2821 cgcggtcgtt ttcaggtaca gacgcggctc gtccaatcgt caggcggtct aagctcatgg
     2881 ttctcctcca acccttcgcc ttcggctcag ggcaagcgtg cttctcgggt ttcgcgccga
     2941 tccaaccggg gctaacaaac ctatccgaaa agtggagtgc agacttcaag ctgcatggcc
     3001 caagcgggct aaagccccgc accccggggt ttccggttcg gctctaagtg taggcggagc
     3061 gcgtgaatag agtatgagga agtgattaaa gaatctgaac aggcagatgc cgccaaacct
     3121 acgctaaacc gacgaaagac gcctcgaagt ggcgtcaact tgacttaact ctaccgcgcg
     3181 gcctgattcg agcgcatttc ctcatttata acgtgcaaac tgtggtgaag gttccatttg
     3241 cctcgaatcg accacaacct tacggctcga cgataacttg cacgaagccc gtgaacaacg
     3301 gccccggccc gaaggtgtcc ggccggccgt cggcgttggc gtcacggccg tcggtcaatt
     3361 cggtatcggc gctggcgccg acttcgacaa tgtgactgcc cggctcgagc tgcggcatgc
     3421 tgtaagacca tcgcgcctgc gccccctctt cgatcgactc gaccggtccc cagtattgcc
     3481 ccgggtcttc gagcggcttt ccgtcgacgg tcacgctcat gctgattgat gaaaggaaag
     3541 tgtcgacgag aggcccctcg ctggccttcc acagccacac gacgcgcacc ggctgaccct
     3601 gtcgcacctc gcgcgtgcag ttgcataatg gcgagacgac cacgtcgccg gtcgacggct
     3661 ggccgtgcac cgacaggtta tcgaacgcgc agcgcgcgag cggcgggcgc gaagcatccg
     3721 gctcgcacgc aaacccgatc gcgcccggcg tgtatgcgat cgacgcctgg agcacctgca
     3781 cgccgttcaa ccagacgctc aggaggtcgc cggcgacgat gatcttccag tggttgacgg
     3841 cgtcgccggt tcgaatcgct tcggtcggcc gatcgaggac gatggcctgc tgctccccct
     3901 cgatgcgctt gtagaacgag agcagcccgt cggccgaggc gtagacgatg tagttgttgt
     3961 cgaggtcctg aacgccgaac atcacgccgg cccggctgcc gcccgatcct tcaagggcac
     4021 ggctgtccac ctccagctcg aagtcgccgg tggcgaacat ggggttccat tgcaggagcg
     4081 cctggcgcgg ctccagggag ggcacgctca actggaactg gccgtcttcg atgaaggcgg
     4141 ccagcccacc cgggtagcgc accgcgcccc acaagttgcg attgtcgtcg aacgcatcca
     4201 cgagcgagac ctcggtcggc gagagcgact cgggcgcggg gcagacgcgg aagttgtcga
     4261 agcgcgcatc cacgccgccc ttctcgaacg acgcggccgt caggccgatg cgtgacccgg
     4321 acgaggacgt gcctggcgcg tcgccgacca ccgcgagcag cacgccgttg ccgtagagcg
     4381 ccagcgtgtc tcccttgtga acggcgcgca agcgattgac gacgttgccc gtgtggagag
     4441 acggcgcaaa ggtccagtct cgggcgctcc tggcgccgtc cgggtcacgg cgcatcacgg
     4501 cgaaattctg cgcgctgtcc acgacgaaag ccgtgtaagt gttcacggct gcgtcggcgc
     4561 cgaggaccag cccgtagtgg cccggcgctt ccgacgccag gtaagcatcg acctcgaccg
     4621 cgtaatcggc gaaatcctgg cggagcgcgc gcgcctcgag ctcgttggcg agcttgatca
     4681 gcatctggta ttcaccgccc acgtatcccc aggcggcgtg gttgttctcg ccgcgtggaa
     4741 agcccccgcc cggatcgctg aaatcgtcaa acacgatcgg ctcgcaggcg gcgggcgcgg
     4801 ctgctgcttg ccccccctcc ggctcccccc gttctgacgg ggggagggag agggggggcg
     4861 cggtcggcgt gccgttggag agcggaagga tattcggctc gaacgtgacg ccgccggacg
     4921 ggctatctgg cggcggtagg ctgccgacca cgacggccag gagtccgatg agcgcgaaga
     4981 agacgaccgc gacgagcacg cccgcgagcg cgggccgggg caggcgaagc ggcttgcggg
     5041 tgacgatagt cgggcggagc ggagcggctg gttgtgtcgg cgcttgcctc gcgacgcgcg
     5101 gctgcgtggc gggattcagt cctgtccgga tcgcgtcgtc aaacgcggcg gccagctcgc
     5161 cggcagtggc atatcgatcg ccgggtttct tcgccagcgc cgtgtcgagc actctctcga
     5221 cagattcggg caggtcgggc gcgtgcgcgc gagccgaagg cagcggctcg gtgacgtgct
     5281 tgaggactac cgccagcggc gtttccgcgt cgaacggcac gcagccggcg agcgcttcgt
     5341 acagcatcac gccgagcgag tatacgtcgc tgcggccgtc gatctcgaac ccctgtccct
     5401 gctccggcga catgtactgc ggcgtgccga tcacgctgct gcccgtgagg tcgagcgtgc
     5461 cggccaccat cttggcgatg ccgaagtcgg tgaggaaggc atccccctgc cggtcgagca
     5521 gcacgttgct cggcttgatg tcgcgatgga tcacgccctg ccggtgcgcg taatccagcg
     5581 ccgaagcgat ctgggagagg accttgctca cttcccgcag aggcatgcgc ccggcgtcga
     5641 tctttttttg cagcgacccg ccgtcgaggt agcgcatgac gatgtagacg acgccgtcgc
     5701 tctcaccgta atcgtagacc ggcagaatgt gagcgtgctc aagccgcgcg atgacctgcg
     5761 cctcctggcg gaagcgcgcc aggaaggtcg cgttctgcgc cagcaggggc ggcaggacct
     5821 tgacggcgac cgtgcggttc atgccgggct ggaaggcttt gtagaccgtc gccatcccgc
     5881 cctggccgat cggctcgccg atttcgtatg cgccgagttt ctttccgatg aattcgaaca
     5941 tggcgtgtgt gagcagccat gctcattata ctcttgtttg ggaatgagtt tgaaggccct
     6001 gacagattca ccgtgcggcc atctcgccgt agatcgacag cagttggcgc gcggcggcgt
     6061 cccaggtaaa cttcgaagcg cgccgcaggc cgtcgcgcat gtacgcgtcg cgcatcgacg
     6121 ggtccttcag cagtccgatc agcccggcgg cgatcgcgtc gacgtccgtc ggatcgacca
     6181 gcaggccggc gtcgccgacg acttcgggaa tcgacgacgt gcgcgagccg atgaccggcg
     6241 cgccgcaggc catcgcttcc agcacgggca gcccgaaacc ctcgtacagc gacacgtagg
     6301 cgaacagcgt cgcggccgaa tacagcgccg gcaggtcagg ctcgtcgatg aagccggtga
     6361 acttcacgcg cccttcaagc ccgaggcgtg tcacttcgga gaagatgtcg tcgaacatcc
     6421 agcccttgtc gcccgcgatc actagattca catcgcccaa caggtctgag ccgatttgcc
     6481 accagcgccc caccacctgg ccgaaggccc ggatcaggcg cacgtaattc ttgcgcggct
     6541 gaatggtgcc gacggacaga atgaaccggc catggcccaa attgtacttc gcgcgcacgg
     6601 cggcgagcgc cgccggatcg cgaatcggcc tgaagcgcgc gtcgacgccg ctgagcagca
     6661 cgtcgatctt cccggcgggg acgccgaaca attcgacgag atcgtctttc gtcgcctgcg
     6721 agtcggccag cacgcggccg gcgcgcgcaa ccgatcggcg cacggcgcgg tcgagaaacc
     6781 tgcgcagcga tggcacggcc gagtcgggat cgcggatgaa cgacaaatcg tgaacggtga
     6841 gaagcgtgcg cgtaccggga agcaccggcg gcagcgtaaa gtcgggcgaa tggaacaaat
     6901 cgaggcggcc ggtcgccagc tcgactggaa tgggaagccg aaaacggtgc cagacgcgag
     6961 caagccactt gtcgtgaatg ggtaggcgcc gcaggcgcac acccgccggg agaggggcga
     7021 tgggctgctt gacctttttt tcggtcgcgt agaacagcgt gtagtggtgc ggcgcgccca
     7081 gcgcgaacag cgcgcgcaca agctcgcgcg tgtagcgccc gatgccggcg gactgtgtga
     7141 cggccgaggt gacgtcgatg ccgatgcgca tatcaattcc ctgagttccc ccagttccct
     7201 gagttccctc agttccctgc tctccgatgc gggaaactga gggaactgaa ggaactgaag
     7261 gaactgggcc ttgctgattg tcagtgcccg aactttacgt cgccgtaggt gatgaagcga
     7321 ttggcgaaga agttccagag catcatcgtc accacggcgc aggcgagcgc gaggttgttc
     7381 cccaactgct cgagggtcat gttcagcgtg tcgacggcga agcgtgcgat gaagccgagg
     7441 gggccgtcga gcgatgcggc cgccagcgcg atcaacggct cgtcgagcgc gtcgaaaatc
     7501 agcgtgcgca ccagcaaggc gatgaggttg agaataaaga actgcgcata ctgccgggag
     7561 agcggcttgg agcgcgattc ggggtaagtc cagtaacggt tccaggtgaa gttgctcgtc
     7621 acggccaggg tgaaggaaat ggcctgcgcc agtcgcgcat cgacgtggaa cacgcggaac
     7681 aacaggttga acgcgccgaa gtcgacgacc gcgccgatcg cgccgacgac gaggaacttg
     7741 gcgaagcgct tcgactcgcg gcgcgcggcg gtggagagtg cgtcggaggc agccatcacc
     7801 agccaaactt gacgtcgttg tacgtgatca gccggttcgc cgcgaagttc cataacatcg
     7861 ccacggtcac agccgagact tgcgcgccgt agagcgccag ggccggcccg acgatgggtg
     7921 tgagcagcga gtccacgaag ccaaacacga tcaagttgat gcccagcccc atcaggttga
     7981 gcaggaagaa ctgcgtcatt tgccggcgga tcggcttcga gcgtgactcg gggtaggtcc
     8041 agtagcgatt ccacaggaag ttgctgatca ccgccagcgt gaacgagatg gctttggcga
     8101 tcaagcgctg ccagtccccg gtcaagccga acaggaagac gagcagcgtc agcgcgcccg
     8161 tgtcgatgac gaagccgatg ctgccgacga tggcgaactt catgaagcgc ttggcttctt
     8221 tgcgatgggc cggcgggaca cggcggcgca cgcgctcgcg gatgcgaagc ggcgtgtgaa
     8281 aatcggcgac ctgcgcccgg atcgatttcc gctcaacggg tggcgtgctc atccggcccc
     8341 ccctctcctg tcgtccgcga cggcctgccc tcgcctgctg tgtgcggggg gagaggggcc
     8401 gggggtgagg gccgccgcgc cgatcgcgct caacaagcca atcatcgcgc cgatatggag
     8461 atggacgttg ttgacgtaca ggttgtcgaa caagtggtgc acggacaaat ggacccacgc
     8521 gccgagcagg cccagcgcca gccagcgata tgggcccgac tgcctgtcca acgtgcggat
     8581 cgtcagcgcg aagatggcag tccacaggcc gagataggcg agcaggcccg gcaggccgat
     8641 ctccgccgcg acgttcaaat agatgttgtg cgcgtgcccc agcggcatcg gccagttcaa
     8701 caggcgatac tgctcgtaca cgggctggta attggagaac cccacacccg accacggatg
     8761 ggtctcgatc attcgcacgg cggcctgcca gtgcgccagt cgctcgacga cggcgtagtt
     8821 ggcgtcgttg atcttcacgc cgcgcacgtc gcgaaagtcg accagcgcgc ccacgtctgc
     8881 aaagcggtcg gcgatcgagg caggcagcag gttcgcgccg tacaatccca gcccgatcac
     8941 gaccagtccg ccgatcaccg ccagcccgat ccaccggcgc cggggcaaaa atagccccat
     9001 cgccaggagc gcggccgccg cgccgagcca cgccccgcgt gaaaatgaca ggtagagtgc
     9061 agtggccagg aggaggacgg tgaacgagac gagtgaagcg tgaaacgtaa aacgtaatat
     9121 ccgctgcctg aaacctggca catcatacgt ggcacgcgcc tgccaggcgc ttccccacaa
     9181 tgccggcgcg agggcgatgg cgatgggcag gttgaggccg atgaatccgg cgaacgggtt
     9241 gggctgctcg aacgagccat acgcgcgata cacgccctcg gacagcagga agccttccgg
     9301 cccgacgcct cgaatgcgcg cttcgtaaat tccgacgagc gcctgcacgg cggccgagag
     9361 cagaaggccg gcgacgatcc acttcgcccc tcgccgccgc acgacgtcga cggcgaacag
     9421 cgcgatcagg atcagctcga tccacttcaa gacctccggc agcccgaacg aatagtcggg
     9481 cgcccacagc atcgtgatcg ccgcccagcc ggtgaagatc gacaagggga tgagcagcgc
     9541 catgcgcgga atgtcaagct cgcgccggat caggccccgt ccgacccacg cgccgagcac
     9601 gagcgcgacg gtcaattgtc cggcgtcggt cggcagcagg ccgggcaggt acgtgttctg
     9661 ccacgccgcc cacggcgcga cgacgagcaa ggcgatcaat ccgagcgccg gctcgatcag
     9721 cgcgcccgcg acgacggagc caacgccaag cgcgacgacg gccgcagaca gcggcaggcg
     9781 cgcaatgaga acgcctccga gcacggccag cagcggaagc gtgaaacgtg agacgtgata
     9841 acgtgaaacg tgataacgtg atgagtgaac cgtgatgcgt gacacgtgaa tccgataagc
     9901 gataagcgat acgcgatacg tgatgagcga taagctagac gcgcgttcgg aagtgctcga
     9961 tcgtccggcg cagcccctct tcgagcggga ctttcggctc ccagccgagc aactgcttcg
    10021 ctttggagat gtccggctgc cgtgtctgcg gatcgtcctg gatgcgtttc tcgggctggt
    10081 agaccacacc cgccgtgctg cccgtcaagc ggttgatcac ctcggcgaat tcgatgatcg
    10141 aaagctcggt cgggttgccg aggttgaccg gctcgtgctc gtcgctgaac aacagccggt
    10201 agatgccttc gatcaggtcg tctacatagc agaatgagcg ggtttgcttg ccatcgccgt
    10261 agatcgtaag tggttcgcct cgcagcgcct gcgccacgaa attcggcacg acgcggccgt
    10321 ctgcaagcct catcctcggc ccctgggtgt taaagatcct aacaatgcgc gtatctacgc
    10381 cgtgatagcg gtggtaagcc atcgtcatcg cttcggcgaa gcgcttcgcc tcgtcgtaga
    10441 cgccgcgcgg gccgatcggg t
//
LOCUS       DBNHXB010000087         5043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1358111, whole genome shotgun sequence.
ACCESSION   DBNHXB010000087 DBNHXB010000000
VERSION     DBNHXB010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1358111"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="AC162G_03320"
     CDS             complement(<1..524)
                     /locus_tag="AC162G_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR5074879.1"
                     /translation="MVDVIRYEELTFPEVAKLPHDVPMLIPLGEADGYDWQAVADRVQ
                     ERTRRRVDRVCVFPGVPFGFRTRQPALKRLAVDPGLLRRVLASLIASVREDGFTRMFV
                     VNGQGAQGFAARDVPMIVGKEAGRPAPPPTLNWGSAQENAARVALIATGHTEQHALHL
                     PLNTDTLIIGAIAEG"
     gene            complement(602..1264)
                     /locus_tag="AC162G_03325"
     CDS             complement(602..1264)
                     /locus_tag="AC162G_03325"
                     /inference="COORDINATES: protein motif:HMM:NF020419.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HIR5074880.1"
                     /translation="MALLKNPHWEAVDPDLRKVLEIVSQQPFANRIYLAGGTALALQI
                     GHRRSKDLDFFSYEDEFQDASRREITSALEQVCQVERREDVIGNLLLYVNGVSTGFFS
                     YGYPLLEAVELSVPIRLASIADIGLMKLDALITRGARKDFIDLFFVLRLMSMDDLIAL
                     RPVKYPRFQNFEMMLLEGLTNHENSERDYMPDMLLSVEWNEVKTTFLAEARRLGSRWF
                     NS"
     gene            complement(1252..1566)
                     /locus_tag="AC162G_03330"
     CDS             complement(1252..1566)
                     /locus_tag="AC162G_03330"
                     /inference="COORDINATES: protein motif:HMM:NF047062.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6922 domain-containing protein"
                     /protein_id="HIR5074881.1"
                     /translation="MRDEAELSAGGIPVSLKPFFQEYDFGKLDADQHQDLVIERTLGY
                     GDQREVRWLFARYGRKLIVEWVRQRGAARLPRRRFNCWQALLSITEFEHPQYWRQSVW
                     PY"
     gene            complement(1577..2647)
                     /locus_tag="AC162G_03335"
     CDS             complement(1577..2647)
                     /locus_tag="AC162G_03335"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HIR5074882.1"
                     /translation="MIPIIDAKGTHHQIGFAIGRKVGDRVARMVVNYRHLLEQTPEIK
                     LTWQRAVVQARKYLPFAVEYMPQYVDELRGIAEGSGADFEDLMVCNCLEELTSDMLFE
                     RCTVVAFGPDHTQSAHVLIGHNEDWMPVDQDLQYIVRAEPEGELPFLALTYGGLLCNI
                     GLNAAGIAQCINSVYPNDMRLGIPRVFIGRAVLAAPRIGRALYRAAHPRRAAGYNHVL
                     ADESGELYNVEATSGLFDPLYGGDEGYLTHANHYTSPMLRPYEEQPHQLTGSHVRDNR
                     ARRLVKQLARRGNVTVEDVQTILSDHVNYPASICCHSKDLPILDDTATIGSLVIDLNA
                     RTMWYCYGNPCEGEFAPLTMKG"
     gene            complement(2650..3414)
                     /locus_tag="AC162G_03340"
     CDS             complement(2650..3414)
                     /locus_tag="AC162G_03340"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004595063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR5074883.1"
                     /translation="MSNYTSSEMMVVAASRLLRDGDVVFVGIGLPNLACNLARRTHAP
                     NLNLVYEAGVIGAQPARLPLSIGDPTLVSGAAAVCSMYEVFAFYLQNGKIDVGFLGGA
                     QIDRFGNVNATVIGAYDKPKVRLPGSGGSAEIAAWANRTYFITPHQARRFPEKCDFVT
                     GAGFLSGRAERRATGVRGGGPHAVVTDLGILTPDEETGELVLTRVHPGATAEQAVKNT
                     GWPLRVATDLQTTEPPTDEELQLLREELDPTGIYVR"
     gene            complement(3411..4013)
                     /locus_tag="AC162G_03345"
     CDS             complement(3411..4013)
                     /locus_tag="AC162G_03345"
                     /inference="COORDINATES: protein motif:HMM:NF020419.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HIR5074884.1"
                     /translation="MPIPRDFYLGGGTALALQIGHRISHDLDFFSATNPLGMEEREVL
                     AGHLQRLKPMTIRHESDAQLYATILGVEVSFIYQHHPLLFPTIDLDGIEAAQPADIGL
                     MKLSAIKDRGTRRDFVDLYAMRLVAPMKTLFQLLPQKYYDRPDFTVHLAYALRYFEDA
                     EADPRELHLLQKLSWSAVKKYCTEGARLLSKINAGLEPPA"
     gene            complement(4079..4393)
                     /locus_tag="AC162G_03350"
     CDS             complement(4079..4393)
                     /locus_tag="AC162G_03350"
                     /inference="COORDINATES: protein motif:HMM:NF047062.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6922 domain-containing protein"
                     /protein_id="HIR5074885.1"
                     /translation="MAVELAPNGLPRSSEWLFPEYDFAGMNPDTYAGVVIERILERGS
                     WAEIRWALDRYGRRGIAKWVRKHGYRRLDRRAFEYWRWMLGIQRYRVPPWESMRRSRK
                     GE"
     gene            complement(4401..>5043)
                     /locus_tag="AC162G_03355"
     CDS             complement(4401..>5043)
                     /locus_tag="AC162G_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5074886.1"
                     /translation="HILGGQAFPSAHRLAHRCFPEAMSANRPLDEIRRFDGEYYVERD
                     PPFEFQPAHELVEDGWTGDLGDVAFTLVHLPGHAPDMIGVHIPMEKTLFAADMLSDVE
                     LPMIDGDGGDYLASLRKIDALVASGQVETLVPGHGHVTRGADRIRARIIEDTEYINQL
                     RMVISGTLNEDASEDTAVKACQTMPHRGKDGWPPMGKVHEANVRKIYRSLLVR"
BASE COUNT          974 a         1579 c         1463 g         1027 t
ORIGIN      
        1 ccctcggcaa tcgcgccgat gatcagagta tcggtgttca gcggcaggtg caaggcgtgc
       61 tgctcggtat ggccggtggc gatcagcgcg acgcgcgccg cgttctcttg cgccgatccc
      121 caattcaagg tgggaggcgg cgcgggtctc ccggcctcct tgcctacgat catcggcaca
      181 tctctcgccg cgaatccctg cgcaccctgc ccgttgacca caaacatgcg tgtgaagccg
      241 tcctctctca cgctggcgat cagcgaggcc aacacgcgcc gcagcagtcc tggatcgacg
      301 gccagccgct tgagcgccgg ctgccgcgtc cgaaagccga agggcacgcc ggggaacacg
      361 cacacccgat cgactctgcg ccgcgtccgc tcctgcaccc gatcggccac cgcctgccaa
      421 tcgtagccgt ccgcctcgcc gagtgggatc agcatcggca cgtcgtgcgg cagtttggcg
      481 acttcgggaa atgtcagttc ttcgtagcgg atgacatcga ccatagcgtt actcctggtg
      541 agctgcgaac gacggccaaa gtcgagtcga agagatagcc accatggatc gtctccaggt
      601 atcaggagtt gaaccagcgc gatcccaagc gccgagcctc agccaaaaat gtcgttttca
      661 cttcgttcca ctcgacggac agcagcatat ctggcatgta gtctcgctcg ctgttttcgt
      721 gattggtcaa tccttctagc agcatcattt caaaattctg gaatcgcgga tacttgaccg
      781 gcctgagcgc aatcaaatcg tccatcgaca tcaatcgcaa gacgaaaaag aggtcaataa
      841 aatcctttcg tgcgcctcgg gtgatgagcg catccagttt catcaacccg atatccgcga
      901 tcgatgccag gcgtatcggc acagacaatt cgactgcttc aagcaaggga tagccatagc
      961 tgaaaaaccc ggtgcttacg ccgttgacgt atagcaacag gttcccaatc acgtcctcgc
     1021 gtctttcaac ttggcaaacc tgctccagcg ccgaagtaat ctcgcgccgt gaggcatcct
     1081 gaaactcatc ctcgtatgaa aagaagtcga ggtcttttga ccggcgatgt ccgatttgca
     1141 aagcgagcgc cgtgccgccg gccaggtaga ttcgattggc gaaaggttgt tggctgacga
     1201 tctccaatac tttgcggaga tctggatcga ccgcttccca atgtggattc tttaataggg
     1261 ccatacggac tgccgccaat attgtgggtg ctcgaattcg gtgatgctca acagtgcttg
     1321 ccagcagttg aaccggcggc gcggcaagcg cgctgcccca cgctggcgta cccattcgac
     1381 aatcagcttg cgcccatagc gagcaaacaa ccagcgtact tcacgctgat cgccgtagcc
     1441 cagcgtccgc tcgattacca agtcttgatg ctggtcggcg tccagcttac cgaagtcata
     1501 ttcctgaaag aacggcttga gcgaaaccgg aattccgccc gcgctcagtt ctgcttcatc
     1561 cctcatcctt ccgccttcat cctttcatcg tgagcggcgc aaactcgcct tcgcatgggt
     1621 taccataaca ataccacatc gtgcgcgcgt tcaggtcgat cacgagcgat ccgatggtcg
     1681 ccgtgtcgtc gagaatgggc aagtcttttg agtgacagca gatgctcgcc gggtagttga
     1741 cgtgatccga taggatggtc tgcacgtctt cgacggtgac gtttccacgc cgtgcgagct
     1801 gcttgaccag gcggcgggcg cggttgtcgc gcacatgcga gccggtcaac tggtgaggct
     1861 gctcttcgta ggggcgaagc atcggcgacg tgtaatggtt ggcgtgggtg aggtatccct
     1921 cgtcgccgcc gtagaggggg tcgaaaagcc ccgacgtggc ttcgacgttg tacaactcgc
     1981 cgctctcgtc agccagcacg tgattgtagc cggcggcgcg ccgtggatgg gcggcgcgat
     2041 acaacgcccg cccgatgcgc ggcgcggcca ggacggcgcg cccgatgaac acgcgcggga
     2101 tgcccagccg catgtcgtta gggtacaccg agttgatgca ctgcgcgatg ccggctgcgt
     2161 tgagaccgat gttgcacagc agcccgccgt acgtcagcgc caggaaaggc agctcgccct
     2221 ctggctccgc gcggacgatg tattgcaggt cctgatcgac cggcatccag tcttcgttgt
     2281 ggccgatcag cacgtgagca ctttgggtat gatccggccc gaatgccacg acggtgcagc
     2341 gctcgaatag catgtcggac gtaagctctt ccaggcagtt gcacaccatc aggtcttcga
     2401 agtccgcgcc cgatccttcg gcgatgccgc gcagctcgtc tacatactgt ggcatgtact
     2461 cgacggcaaa cggcaggtac ttgcgcgcct gcacgaccgc gcgctgccac gtcagcttga
     2521 tctcgggtgt ttgctccagc aggtggcggt agttgacgac catacgcgcg acgcggtcgc
     2581 ccaccttgcg gccgatggcg aagccgattt ggtggtgcgt gcctttggcg tcgatgatgg
     2641 gaatcatgtt tacctcacgt atatccccgt cgggtcgagt tcttctcgca gcaactgcag
     2701 ttcctcatcc gtaggcggtt cagtcgtttg gagatcggtt gccaccctca atggccagcc
     2761 cgtattcttg accgcctgct cggccgtcgc gccgggatgc acacgtgtca gcacaagctc
     2821 gccggtttct tcatccggcg tcagaatacc gagatcggtg acgacggcgt gcgggccgcc
     2881 gccgcgcacg cccgtcgccc ggcgctccgc gcgacccgac aggaagcccg cgccggtgac
     2941 gaagtcgcac ttctcgggga aacggcgcgc ctgatgcggc gtgataaagt acgtgcggtt
     3001 ggcccacgct gcgatctcgg cgctgccgcc gctgcccggc aaacgcactt tcggcttgtc
     3061 gtacgctccg atcaccgtcg cgttgacgtt gccgaagcga tcgatctgcg cgccgcccag
     3121 gaagccgacg tcgatcttcc cgttctgcag gtagaacgcg aagacttcat acatcgagca
     3181 cactgccgcc gcgccgctga ccagcgtcgg atcgccgatc gacagcggca ggcgcgccgg
     3241 ctgcgcgcca atgactcccg cttcgtacac cagattcagg ttgggcgcat gcgtgcgccg
     3301 cgccaggttg caggccaggt tgggcaggcc gatgcctacg aacaccacgt cgccgtcgcg
     3361 cagcagccgc gacgcggcca cgaccatcat ttcggacgaa gtgtagttgc tcatgcaggc
     3421 ggctccaatc cagcattgat cttcgacagg aggcgcgcgc cttccgtaca gtatttcttg
     3481 actgcgctcc aactcagttt ttgcagcaag tgcagttcgc gcggatcggc ctctgcgtct
     3541 tcgaaataac gcagggcata agctagatga acggtgaagt ctgggcggtc gtaatacttc
     3601 tgtgggagaa gctgaaatag tgtcttcatg ggggcgacca gacgcattgc gtacaggtcg
     3661 acaaagtcac ggcgcgttcc ccgatccttg atcgcagaca gcttcatcaa gccaatgtcg
     3721 gccggctgcg ctgcctcgat gccgtcgaga tcgatcgtcg ggaaaagcaa gggatgatgt
     3781 tgataaatga aactgacttc cacgcccaag atcgtcgcat acagttgggc gtcggactca
     3841 tgtcggatcg tcatgggctt caggcgctgc agatgacctg ctaacacctc gcgttcttcc
     3901 ataccaagcg ggttggtcgc cgagaagaag tcaaggtcgt gcgaaatgcg atggccgatt
     3961 tgcagtgcca gcgccgttcc gccgccaagg tagaaatcgc gcggtatggg caacttcgcc
     4021 agggcttcaa acgtgcgtct ggtctccggc gcgagagcgt tccagaaaat gtgagaggtc
     4081 attcaccttt cctcgaccgc cgcatcgatt cccatggcgg cacgcggtat cgctggatgc
     4141 ccagcatcca gcgccagtat tcgaatgcgc gccgatcgag ccggcggtaa ccatgctttc
     4201 gaacccattt ggcgatgccg cgtctgccgt atcgatcgag cgcccagcga atctctgccc
     4261 atgatccacg ttcaaggatt cgctcgatga ccacgcctgc atatgtgtca ggattcattc
     4321 cagcaaaatc atattctgga aacagccatt cgctcgagcg aggtaatccg ttaggagcca
     4381 gttctaccgc cattgcgccc tcatctaact aacagactcc gatatatctt cctcacattc
     4441 gcctcatgta ctttgcccat cggcggccag ccatccttgc cacgatgcgg catcgtttgg
     4501 caggctttca ctgccgtgtc ttcgcttgca tcctcgttca gcgtgccgct gatcaccatc
     4561 cgcaactgat tgatatattc agtatcctcg atgatccgtg cacggatcct atccgcgccg
     4621 cgagtgacgt gcccatgtcc cggcacaagc gtctcgacct gaccagatgc gacgagcgcg
     4681 tcgatcttgc gcagcgaagc gagatagtcg ccgccatcgc catcgatcat cggcaattct
     4741 acatccgata gcatgtcggc ggcgaacagc gtcttctcca ttgggatgtg gacgccgatc
     4801 atgtcgggcg catgccccgg caagtggacc agcgtgaacg cgacgtcgcc caagtcgccc
     4861 gtccagccat cctcgaccag ctcgtgcgct ggctggaact cgaacggcgg atcgcgctcg
     4921 acgtaatact cgccgtcgaa ccgccgaatc tcgtccagcg gccggttagc gctcatcgct
     4981 tccgggaaac atcggtgcgc caatcgatgc gccgatggaa acgcctgccc gccgaggatg
     5041 tga
//
LOCUS       DBNHXB010000088        11127 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_183986, whole genome shotgun sequence.
ACCESSION   DBNHXB010000088 DBNHXB010000000
VERSION     DBNHXB010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11127)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11127)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11127
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_183986"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="AC162G_03360"
     CDS             complement(<1..1001)
                     /locus_tag="AC162G_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074887.1"
                     /translation="MSRPSYLIILAGLLALSLSVKEAAPRPATYTSPGEQVAQALTIT
                     STPAQPPEAPRYTGIRGSAAQQAVVNITELARQESAAGHRTIEPIVIPYLPIPGNLPV
                     TLQSTPQPAPASGVSDVPLPGPLAPQSPAPASSFPALEDNNSVIPPDTHGAAGPNHLM
                     VTLNSQVRIQNRSGAALTTVSLNAFWSAVAGSSGVFDPKVLYDPYGGRWIFTACDDAQ
                     RASSALLIGVSQTSDPTGNWNLFRIDSDAANRAWADYPSLGFNKDWIVVQVNMFSVSN
                     NMFNRTHIYVINKANLYSGANANHTLFSLTGISATQVPAITYDNALATMYLLQNWDG"
     gene            1395..2362
                     /locus_tag="AC162G_03365"
                     /pseudo
     CDS             1395..2362
                     /locus_tag="AC162G_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527054.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0008076 - voltage-gated potassium channel
                     complex [Evidence IEA];
                     GO_function: GO:0015459 - potassium channel regulator
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel beta subunit family protein"
     gene            2387..4675
                     /locus_tag="AC162G_03370"
     CDS             2387..4675
                     /locus_tag="AC162G_03370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR5074888.1"
                     /translation="MAEYKAIGRPTPLIDGRAKVTGALQYAPDVKVPGMLHARFVMSV
                     YAHARIRAIRVEGALSAPGVVAVLTAKDLPAIVPSSRSRLLLARDRVIFAGQPVAMVL
                     AESEAAAEDGVQHVVVDYEPLPAAITMDEALAEGAPAVWPRGVPKGASDAGAHGADHG
                     GDKKSDKDATNVAETDSFTRGDVDAGFAEADAIVERTFTTPIVHQSSIEPQAVIAQPD
                     PITGGMTVWASTQTPFGVRKEVAEVLGVPESDVRVHGTPVGGGFGGKNGLYEPLAAVA
                     ARAVGRPVQLVLTRTEEMLAGNPAPAVRVRARIGAKRDGTLTALQAKVEVDDGCYPFD
                     LAGFLGYMLGSFYRVPNFHIEAIDVLTFKPSAGAYRAPGALSAVFALDCLLDELAQKL
                     DIDPIEMRLHNAARAGDRMANNKDTWPTIGMRQVLEALRDHPAWQNRARARAQGRGVG
                     IAVGGWLGGTEPAAAICSLHRDGYLHIHVGSADLNGTATGMALIAAEAFGIAPEKVRV
                     KISDTETAPYAGASAGSKVTYTTGAAVMLAAREARQQVLSIAGDELEAAVEDLEIVGG
                     KVQVRGVPDKAIPLTELAEKTMEFGGKYAPVFAHGRLAQAAEAPAFNAQLVEVEVDRE
                     TGAVRLHRLVAVQDVGRAINPLAIQGQMLGGATQGIGWALYEKMDYDAQGQLLTGSWM
                     DYAIPGIGQTPPAMETVIVEVPSENGPFGARGVGEAPVVPAAAAIVNAIADATGVRLS
                     DLPMTAPRVFAALRQRIASHNG"
     gene            4696..5928
                     /locus_tag="AC162G_03375"
     CDS             4696..5928
                     /locus_tag="AC162G_03375"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR5074889.1"
                     /translation="MKWVHLLLFVLLVSACTQSQTSTVSPTEVRPTIGSPAIAPTEPA
                     PTPVEPAATRPPAAPESTPPAVRPAFTLGVEPVASGFERPTYVTHAGDDRLFVVEQAG
                     SIWIIEDGAVLDVPFLSIASKVNSRANERGLLSVAFDPDYTSNGFFYVNYTRQPDGAT
                     VIERYQVSDNPDVADPESGAVLLTIDQPEANHNGGQLQFGPDGYLYIGTGDGGGAGDR
                     HGEIGNGQDDNALLGKMLRMHVRSGEVSIWAKGLRNPWRFSFDRATGDLYIADVGQNV
                     YEEVNFQAASSAGGENYGWRIMEGNHCFNPREGCDQSGLTPPVAEYDHGSGCSITGGA
                     VYRGAAYPGLDGLYFFADYCSGIMWSLERDPSGRWNMAEQMRVNFNVSSFGEDEAGEV
                     YLAGHDDGTIYRLTSASP"
     gene            5956..6921
                     /locus_tag="AC162G_03380"
     CDS             5956..6921
                     /locus_tag="AC162G_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR5074890.1"
                     /translation="MPIPNVKFKIAAVQAAPVFLNREATLDKACELIAEAGRGEARLV
                     AFPESFVPAYPDWVWAVPAGEEGILNELYAELLANAVEIPSVATQRLCQAAKGAGTYV
                     VIGVTERNVEASGASLYNTLLYLDALGNVLGKHRKLVPTGGERLVWAQGDGSTLEAYD
                     TPFGKIGGLICWENYMPLARYAMYAWGTQIYIAATWDRGQPWLSTLRHIAKEGRVVVI
                     GCCMALRKSDIPDHFEFKQRFYQHAGEWINVGDSAIANPDGEFVAGPLREQEGILYAE
                     VDPAQLRGPKWMIDVAGHYARPDVFQLTVNREPRRMIRTEGVMDS"
     gene            7141..9132
                     /gene="nusA"
                     /locus_tag="AC162G_03385"
     CDS             7141..9132
                     /gene="nusA"
                     /locus_tag="AC162G_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR5074891.1"
                     /translation="MKSEFLLAFNQICNERNLPKEVVLEALANALVSAYRKNVNASSA
                     QTITAEIDGDTGQAHIYAEKEVVDFVTDLRTEVALADARDVDEDAKIGDMVMVDSTPE
                     DFGRIAAQTAKQVILQRIREAEREAQYAIYAEREGEIINGTVQSVSAMGVTLNLGRTE
                     ALLPKNQQVPGERYQLHQRIRAYVLEVRKSSRGPQIVVSRNHKNMLRRLLELEVPEIY
                     NGTVEIKSIAREAGARSKIAVAATQAGVDPVGSCVGMRGVRIQSIVNELGGEKIDVIE
                     WSGDPATYIAKALSPARVLTVHVDDDLKDGKTALVVVPDDQLSLAIGREGQNARLAAK
                     LTGWRIDINGAMESIRSALKHIEEDAALRDEMGEAKDVIPTVEAVLQRHAEHSLPWNT
                     DELQAIRKVIDGVQRAIHERRVAEREREHAAEKARRAAAQILEAPLEAGRQEQVEAYR
                     ARIPPAAFDTPLEALELSARVMKNLLRSDLAHVGHIHERLSLGDEAMLAIDGIGPKAL
                     AEIKQAIEAKGLGLIPAVEPVAEEAPVPPAAEVEPEPAAVEVALPQPEAGAPEVQVAQ
                     EAAPALSEAEGAPPAAEVVAEPAPLIVPAMPSVFVPVVDEEEEEAEEGEAGPPGGKKK
                     SKKKGKRKDRVLVYDENLGEVIAVKGRKRGQDVWSEDEA"
     gene            9181..9474
                     /gene="rnpM"
                     /locus_tag="AC162G_03390"
                     /gene_synonym="ylxR"
     CDS             9181..9474
                     /gene="rnpM"
                     /locus_tag="AC162G_03390"
                     /gene_synonym="ylxR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P modulator RnpM"
                     /protein_id="HIR5074892.1"
                     /translation="MPGKHIPQRTCVGCGEVQGKRQLMRVVRTPDGRVHVDPTGKANG
                     RGAYVHERRACWEKALHGRLGHALKIERLSDDDRAALERHAAALSDDTREAVT"
     gene            9524..>11127
                     /gene="infB"
                     /locus_tag="AC162G_03395"
     CDS             9524..>11127
                     /gene="infB"
                     /locus_tag="AC162G_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR5074893.1"
                     /translation="MSTNGKKVVEVPSVVTVRDLANLVKVSPINVIKELMNNGIMANI
                     NQQLDYDTAAIVLTELGFEPREQRPAEAETAADAQRASPATLWQRLYAGEDPKKLKPR
                     PPVVTVMGHVDHGKTSLLDAMRHTDVASGEAGGITQRIGAYQVEHNGRKITFLDTPGH
                     EAFTAMRARGAQATDLAILVVAADDGVMPQTKEAIAHTRAAGVPIIVALNKIDKPNSN
                     PERVKKELHDDGVVIDEYGGNILLVPVSAKKKTGIDDLLEAILLVADTMEIKANPDRP
                     AVGTVIESKLDKSRGAMATVLVQNGTLAVGDPVVIGKSYGRVRAMMDYNGRAIQKATP
                     STPVAVLGLSDVPNAGDVLEVVKNERVARERAAERSAAEGTAASGADRSKVSLEDFFH
                     RAQAEGNKELLLVIKADGQGSIEPLVSSLEKLDQGGARVKVLHTGTGRVSESDVSLAV
                     ASDAIVIAFNVEVDESARRLADSEGIDVRKYNVIYKLVEDIDKALQGMLEPVYEDKVV
                     ARAEVRQVFKIRNVGFIAGCLVREGAA"
BASE COUNT         2150 a         3338 c         3839 g         1800 t
ORIGIN      
        1 ttgccgtccc agttttgcag caggtacatc gtggcgagtg cattgtcgta cgtgatagcc
       61 ggaacctgag tggcgctaat gccggtcagg gagaagagcg tatgattggc gttggcgccg
      121 gagtacagat tcgccttgtt gatgacgtag atatgcgtgc ggttgaacat gttattcgat
      181 acgctgaaca tgttcacctg cacgacgatc cagtccttgt tgaacccgag gctggggtag
      241 tccgcccagg cccggtttgc ggcgtcgcta tcgatccgaa aaaggttcca gttgccggtc
      301 gggtcgcttg tttgcgacac gccgatgagc agcgccgaac tcgccctctg ggcatcatcg
      361 caggctgtga agatccagcg accgccgtat gggtcgtaca acaccttggg gtcgaatacg
      421 ccggagctcc cggcgaccgc gctccaaaaa gcattgagcg acaccgtagt cagcgctgcg
      481 cccgagcggt tctgaatgcg cacttgcgaa ttcagcgtga ccatcaggtg attcgggccg
      541 gcagcgccat gcgtatcggg tggtatgacg ctgttgttat cctccaacgc cggaaaactc
      601 gaagccggcg caggagattg aggcgcgagc gggccaggca atggaacatc tgatacacct
      661 gatgctggcg ccggttgtgg agtggactga agtgtgacgg ggagattacc ggggatcggc
      721 aggtaaggga tcacgatggg ttctatggtt cgatgtcctg cggccgactc ttgccgggcc
      781 agttctgtga tgttgacgac tgcctgttga gctgcgctgc cgcgaatacc tgtatacctc
      841 ggagcttccg gtggctgcgc cggcgtggag gtgatggtga gagcctgggc aacctgctcg
      901 ccgggcgatg tgtatgttgc aggccggggc gcagcctctt tcacactcag cgacagggca
      961 agcaggccgg ccaggatgat gagatacgac ggacgactca tagaaacacc gccgactcga
     1021 ttgtgggcta gggtgctcgg cggcacgtat tataagcctg aaactgcgac tccggctatc
     1081 tggtgattaa tcatctcgct ctcagcgccg tccccggcgc tggcaatgcc gccgaggacg
     1141 gcggtctgag cgttgtgtgt gatgaccagc ggctatatgc tatactctag ctgccggccg
     1201 gagaacccag cagaggcggt tccttgcgaa gcaccccgta gcgcagttga gtgggcacac
     1261 cgccgattca gcgcgtttca aggcgaattg ccgggcgtgg gagcctggcc cgcgctctct
     1321 ctccgaccct tcgccttcgc cagggcaggc aggctgctgt gccgtggtca aatcaacgta
     1381 gaaagggact cctcatgcgc tatcgccgtc tgggcagcgc cgggttgaag gtctctgaac
     1441 tatcgtttgg cggatgggtc accgtcggcc agcagatcga cgaagagact tctcgccagt
     1501 gcatcctgac cgcctacgag gccggcgtca acttcctcga caacgcggaa gcgtatgcgg
     1561 acggcaagtc cgaaatcgtc atgggccgag tgctcaaaga gctgcgccgc gagagcctgg
     1621 tgatctcgag caaagtcttc tggggcggcg aagggccgaa cgacgagggc ctcagccgca
     1681 aacacatcac cgaagcctgc cgcaactcgc tgaagcgcat gcgggtcgac tacttcgacc
     1741 tgtatttctg ccaccggccc gacccgaaca cgccgatcga agaaaccgtg cgcgcaatgg
     1801 acgacctgat tcaccagggc ctgatcctgt actggggcac gagcgagtgg agcgcgcagc
     1861 agatcatgga ggcgtacgcc gtcgcgcggc agtacggcct caacccgtcg cagatggagc
     1921 agcccgagta caacatgttc acgcgcaagc gcgtcgagaa ggagtacgcg cggttgtaca
     1981 tgcagatcgg cctgggcacg acgatcttta gcccgctggc atccggcgtg ctgaccggca
     2041 agtacaacga cggcatcccg aagggacgcg cgcgtcactc aaaggctacg agtgggtgaa
     2101 agacgatatc acgccggagc gcatcgggaa ggtgaagcgg ctcgcgccga tcgccaccga
     2161 cctgggctcc acgctggcgc agatggcgct cgcctggtgc ctgaagaatc ccaatgtcag
     2221 cacggtcatc accggcgcca gccgcgtgga gcaggtgcgt gagaacatga aggcgctcga
     2281 cgtggtcgag aagctctcgc cggacgtgat ggagcgcatc gaggaggttc tgcagaacaa
     2341 gccgaaggtc ggcgagaatt agcccggtgt agagtgagag gctgtcatgg ctgaatacaa
     2401 agcgatcgga cgccccacgc cgctgatcga cgggcgcgcg aaggtcacgg gcgcgcttca
     2461 gtacgcgccg gacgtcaagg tgcccggcat gctgcatgcg cgcttcgtga tgagtgtata
     2521 cgcgcatgcc aggatccgcg ccatccgtgt cgagggcgcg ctgagtgcgc ccggcgtggt
     2581 cgccgtgctt acggcgaagg acctgcccgc aattgtgcca tcgtcgcgca gccgcctgtt
     2641 gctggcgcgc gaccgcgtga tcttcgccgg ccagccggta gcgatggtgc tggccgagag
     2701 tgaagcggca gccgaagacg gcgttcagca tgtcgtcgtg gattacgagc cgctcccggc
     2761 ggcgatcacc atggacgagg cgcttgccga gggcgccccg gccgtctggc cgaggggcgt
     2821 gccgaagggt gcgagtgacg caggcgcgca cggagccgat cacggcggag ataagaagtc
     2881 tgacaaggac gcgacgaatg tcgccgagac ggactccttc acgcgcggcg acgtcgatgc
     2941 cggcttcgcc gaggcggatg cgatcgtcga gcgcacgttc accacgccga tcgtgcatca
     3001 aagctcgatc gagccgcagg cggtgatcgc tcaacccgat ccgatcacgg gcgggatgac
     3061 ggtgtgggcc agcacgcaga cgcccttcgg cgtgcgcaaa gaggtggccg aggtgctggg
     3121 cgtgcccgag tcggacgtgc gcgtgcacgg cacgccggtc ggcggtggct tcggcggcaa
     3181 gaacgggctg tatgagccac tggcggccgt cgccgcgcgc gcggtggggc ggccggttca
     3241 gctcgtgctg acgcgcacgg aggaaatgct cgccggtaat ccggcgccgg ccgttcgcgt
     3301 ccgcgcccgc atcggcgcga agcgcgatgg cacactcacc gcgctccagg cgaaggtaga
     3361 agtcgacgac ggctgctatc cgtttgacct tgccggtttt cttggctaca tgctcggcag
     3421 tttctaccgc gtgccgaact tccacatcga agcgatcgac gtgctgacgt tcaagccgtc
     3481 tgccggagcg tatcgcgcgc cgggggcgct gtcggctgtc tttgcgctcg attgcctgct
     3541 ggacgagctg gcgcaaaagc ttgatatcga tcccatcgag atgcggcttc acaacgccgc
     3601 gcgggccggc gaccggatgg caaataacaa agacacctgg cccacgatcg gcatgcgtca
     3661 ggtgctggaa gcgctgcgcg atcatccggc gtggcagaat cgcgctcggg cgcgcgcgca
     3721 gggccgtggt gtgggtatcg ccgtcggcgg gtggctggga ggaaccgaac cggcggcggc
     3781 gatctgctcg ctgcatcgcg acggctacct gcacatccac gtcggctcgg ccgatctgaa
     3841 cggcacggcg acgggtatgg ccttgatcgc ggccgaagcg ttcggcatcg ctccggagaa
     3901 ggtgcgcgtc aagatcagcg acaccgagac cgcgccgtac gcgggcgctt ccgcgggcag
     3961 caaggtgacg tatacgaccg gggccgccgt catgctggcc gcgcgcgagg cgcggcagca
     4021 ggtgctctcg atcgccggcg acgagttaga agcggccgtc gaagatctgg aaatcgtcgg
     4081 cggcaaagtg caggtgcgcg gcgtgccgga taaggcgatc ccgctgactg agctggccga
     4141 gaagacgatg gagttcggcg ggaaatacgc gccggtattc gcgcatggcc ggctcgcgca
     4201 ggcggctgaa gcgcctgcct tcaacgcgca gctcgtcgag gtcgaggtcg accgggaaac
     4261 gggcgccgtt cgcctgcatc ggctggttgc cgtgcaggac gtcggccgcg cgatcaatcc
     4321 gctggcgatt cagggtcaga tgctgggcgg cgcgacgcag ggcatcggct gggcgctgta
     4381 cgaaaagatg gactacgatg cgcaagggca actgctgacc ggctcgtgga tggattatgc
     4441 catccccggc atcggccaga ctccgccggc gatggagacg gtcattgtcg aagtgccttc
     4501 ggagaacggc cctttcggcg cgcgtggcgt cggcgaagcg ccggtcgtgc cggctgcggc
     4561 ggcgatcgtc aacgccatcg ccgatgcgac cggcgtccgc ctgagcgacc tgccgatgac
     4621 cgcgccgcgc gtgtttgcgg cgttaaggca acggattgct tcgcacaacg gataaacgga
     4681 tatagaggtc gcgaaatgaa atgggttcac ttgttgttgt ttgtgctgtt ggtcagcgcg
     4741 tgtacgcaga gtcagacgtc aacagtttct ccaacagaag tgaggccgac catagggtcg
     4801 ccggcaatcg cgccgactga gcctgcgccc acgccggtcg agcctgccgc gactcgaccg
     4861 ccggcagcgc ccgaatcaac cccgccggcg gtgcgtccgg cgttcacgct cggcgtcgag
     4921 ccggttgcat cgggctttga gcggcccacc tacgtcacac atgccggtga tgatcgcctg
     4981 ttcgtagtcg agcaggcagg gagtatctgg atcatcgagg acggcgctgt gctcgacgtc
     5041 ccgtttctga gcatcgcctc gaaagtgaac tcgcgagcaa acgagcgggg tctgttgagc
     5101 gttgcattcg atccggacta cacatcgaac gggtttttct acgtgaacta cacccgccag
     5161 ccggatggcg ctacggtcat cgagcgttat caagtgtcgg acaatccaga tgtcgcagac
     5221 ccggagagcg gcgcggtgct gctgactatc gaccagcctg aagcgaacca taacggcggc
     5281 cagttgcagt tcggcccgga cggctatctg tacatcggca cgggcgacgg cggcggcgca
     5341 ggtgaccggc atggcgagat cgggaacggg caagacgaca acgcccttct cggaaagatg
     5401 ctgcggatgc acgtgcgctc gggtgaagta tcgatctggg ccaaagggct gcgcaacccg
     5461 tggcggttct cgttcgaccg ggcgacgggc gatctctaca tcgccgacgt cggccagaac
     5521 gtgtacgagg aagtgaactt tcaggccgcg tcgagcgcgg gcggcgagaa ctacggctgg
     5581 cggatcatgg aaggcaatca ctgcttcaac ccgcgcgagg gatgcgatca aagcggcctg
     5641 acgccgcctg tggcagagta cgatcatggc tccggctgtt cgatcaccgg tggcgccgtg
     5701 tatcgcgggg cggcctatcc ggggctggat gggctgtact tcttcgccga ctattgcagc
     5761 gggattatgt ggtcgctgga gcgcgacccc tcgggcagat ggaacatggc cgagcagatg
     5821 cgggtgaact tcaacgtcag ttcgttcggc gaagatgaag ccggcgaagt ctatctggcc
     5881 ggtcatgacg acgggacgat ttatcggctg acttcggctt cgccctgatt ttccacggag
     5941 gacgaattca tcgaaatgcc aatacccaac gtcaagttca agatcgctgc tgtgcaggct
     6001 gcgcccgtgt ttctgaatcg agaggccacg ctcgataagg cgtgcgagct gatcgcggaa
     6061 gcgggccgcg gtgaggcgcg tctcgtcgcg ttccccgaat ctttcgtccc cgcctatccc
     6121 gactgggtat gggccgtgcc tgccggcgag gaagggatac tcaacgagtt gtacgcggag
     6181 ctgctcgcca acgccgtcga gattccgagc gtggcgacac agcgcctgtg ccaggcggcg
     6241 aagggcgccg ggacctacgt ggtgatcggc gtgaccgagc gcaacgtcga ggccagcggc
     6301 gcgagcctct acaacacact gctgtacctc gacgcgctgg gcaacgtcct gggcaagcat
     6361 cgcaaactgg tgccgaccgg cggcgagcgg ctggtgtggg cgcagggcga cggcagcacg
     6421 ctcgaagctt acgacacgcc gttcggaaaa atcggcggct tgatctgctg ggagaattac
     6481 atgccgctcg cccgctatgc catgtatgcc tggggcacgc agatctacat tgccgcgacg
     6541 tgggatcgcg ggcagccgtg gctctcgacg ctgcgccaca tcgccaagga gggccgcgtg
     6601 gtcgtcatcg gctgctgtat ggctctgcgc aagtccgaca ttccggatca ttttgagttc
     6661 aagcagcgct tctatcagca cgccggcgag tggatcaacg tcggcgacag cgcgatcgcc
     6721 aatcccgacg gcgagttcgt cgccggaccg ctgcgtgagc aagaggggat tttgtacgcg
     6781 gaggtcgatc cggcgcagtt gcgcgggccg aagtggatga tcgacgtggc cgggcattac
     6841 gcgcggccgg atgtgtttca actgacggtc aaccgcgagc cacgccggat gattcggacg
     6901 gaaggggtta tggattcttg agcgcccgag gacgaacgga cttgtggata atcaccggcc
     6961 cggctgaggt tgacagacga acagaaccta gttataatca gcatggtttt ggttataggg
     7021 ctgttgagaa aagtgggcgc tccccacttt tctgcttaaa atagcctgcc ggaggaccgc
     7081 agtcccggct cggcagcgcc gggcacaact gaatatcgcc acggaggaat ggcgaatcct
     7141 atgaagagtg aatttctgct agccttcaac caaatctgca acgagcgcaa cctgcccaag
     7201 gaagtggtgc tggaggcgct ggccaacgcg ctggtgtcgg cctatcggaa gaacgtgaat
     7261 gcgtcgtctg cgcagacgat cacggccgag atcgacggcg acaccggcca ggcgcacatc
     7321 tacgccgaga aagaagtcgt cgacttcgtc accgacctgc gcaccgaggt ggcgctggcg
     7381 gacgcgcgcg acgtggacga ggacgccaag atcggcgata tggtcatggt cgactccacg
     7441 ccggaagact tcgggcgcat cgcggcgcag accgccaagc aagtgatctt gcagcgcatc
     7501 cgagaagccg agcgcgaagc ccagtacgcc atctacgcgg agcgcgaagg ggagatcatc
     7561 aacggcacgg tgcagagcgt gtcggcgatg ggcgtcacgc tgaacctggg gcgcaccgag
     7621 gcgctgctgc ccaagaatca acaggttccc ggcgagcgct accagcttca tcagcgcatt
     7681 cgcgcctacg tgctggaagt gcgcaagagc agccgcgggc cgcagatcgt cgtcagccgc
     7741 aatcacaaga acatgctgcg gcgcctgctc gagctggagg tcccggagat ctacaatggc
     7801 acggtcgaga tcaagagcat cgcccgcgaa gccggcgcgc gctcgaagat cgccgtggcg
     7861 gctacacagg ccggcgtcga cccggtcgga tcgtgcgtcg gcatgcgcgg cgtgcgcatc
     7921 cagtccatcg tcaacgagct gggtggggag aagatcgacg tgatcgagtg gagcggcgat
     7981 ccggccacgt acattgccaa ggcgctgtcg cccgcccgcg tgctgaccgt ccacgtcgac
     8041 gacgatctca aggacggcaa gacggcgctg gtcgtcgtgc ccgatgacca gttgtcactg
     8101 gcgatcggcc gcgaaggaca gaatgcccgg ttggcagcca agctcaccgg ctggcgcatc
     8161 gacatcaacg gcgctatgga atccattcgc agcgcgctca agcatatcga agaagacgcc
     8221 gcgctgcgcg acgagatggg cgaggcgaag gacgtcattc cgacggtgga ggccgtgctc
     8281 cagcgccacg ccgaacattc gctgccgtgg aataccgacg agctgcaggc catccgaaaa
     8341 gtgatcgacg gcgtacagag ggcgatacac gagcggcgtg tggcggagcg cgagcgtgag
     8401 cacgccgcag aaaaggcgcg tcgcgccgcg gcccagatcc tggaggcgcc gctcgaagcc
     8461 ggccgccagg agcaggttga agcttaccgc gctcgtattc cgccggctgc attcgacacg
     8521 ccgctcgaag ctctggagct gagcgcgcgt gtgatgaaga atctgctgcg ttccgacctc
     8581 gcccacgtcg ggcacattca cgagcggcta tcgctcggcg acgaggccat gctggcgatc
     8641 gacggtatcg gtcccaaggc gctcgccgag atcaagcagg cgatcgaggc gaaagggctg
     8701 ggcctgatac ctgccgtcga gccggtcgct gaagaggccc ccgtcccacc ggcagccgag
     8761 gtcgagccgg aaccggccgc ggtcgaggtt gccctgccgc agccggaagc gggggcgccc
     8821 gaggtgcagg tggcccagga ggccgcgcct gccctgagcg aagccgaagg tgcgccaccc
     8881 gcggccgaag tcgtcgccga gccagcgccg ctgatcgtgc cggcgatgcc ctctgtcttc
     8941 gtaccggtgg tcgatgagga agaagaggaa gccgaagagg gtgaagccgg gcctccgggc
     9001 ggcaagaaga agtcgaagaa gaagggcaag cgcaaggatc gcgtgctggt ctatgacgaa
     9061 aatctgggcg aagtgatcgc ggtcaaaggg cgaaagcgcg gccaggatgt ctggagcgaa
     9121 gacgaagcat aaggatacgg agcatccgcc gctacggcgg cgcacgatac atctttttgc
     9181 atgccaggca agcatattcc tcagcgcacc tgtgtaggct gcggcgaggt gcagggtaaa
     9241 cgccagttga tgcgagtcgt ccggacgccg gacggccggg tgcatgtcga cccgaccggc
     9301 aaggcgaacg gacgaggcgc atacgtgcac gagcgccgcg catgctggga gaaagccctg
     9361 catggacgcc tgggtcacgc gctcaagatc gagcgcctga gcgacgacga ccgcgcggcg
     9421 ctcgagcgtc acgcggccgc gctgagcgac gacacacgcg aggctgtcac atgacgggat
     9481 agcgggcggc cgccgcgctt gccgggcacg aggggaggga acgatgagca cgaacgggaa
     9541 gaaagttgtc gaggtgccta gcgtcgtgac ggtcagagac ttggccaatc tggtcaaggt
     9601 cagcccgatc aacgtcatca aggagctgat gaacaatggc atcatggcca atatcaacca
     9661 acaattggat tacgacacgg cggccattgt tctcaccgag ctggggttcg agccacgcga
     9721 gcagcgcccg gccgaagcag aaaccgcggc cgacgcgcag cgcgcctcgc cggcgacgct
     9781 gtggcagcgc ctgtacgccg gcgaagaccc caagaagctg aaaccccgcc cgcccgtcgt
     9841 caccgtgatg ggccacgtcg atcacggcaa gacctccctg ctcgatgcca tgcgccacac
     9901 ggacgtcgcc tccggcgaag ccggcggcat cacccagcgc atcggcgcgt atcaggtgga
     9961 gcacaacggg cggaagatca ccttcctcga tacgcccggg cacgaagcat tcaccgccat
    10021 gcgcgcgcgc ggcgcgcagg ccaccgacct cgccatcctg gtggtcgccg ccgacgacgg
    10081 cgtgatgccg caaaccaaag aggcgatcgc gcacacccgg gcggctggcg tgccgatcat
    10141 cgtcgccctc aacaagatcg acaagcccaa cagcaaccca gagcgggtca agaaagagct
    10201 gcacgacgac ggcgtcgtca tcgacgagta cggcggcaac atcctgctcg tgccggtctc
    10261 ggcgaagaag aagacgggca tcgacgatct gctcgaagcg atcctgctcg tcgccgatac
    10321 gatggagatc aaggccaacc cggatcggcc cgcggtggga acggtgatcg agagcaagct
    10381 ggataagagc cgcggcgcga tggcgacggt tctggtgcaa aacgggacgc tcgccgtggg
    10441 tgaccctgtc gtgatcggca agagttacgg ccgcgtccgc gcgatgatgg actacaacgg
    10501 gcgcgccatc cagaaggcga cgccgtccac gccggtcgcc gtgcttggcc tgtcggacgt
    10561 gccgaacgcg ggcgacgtgc tcgaagtggt gaagaacgaa cgggtggcgc gcgagcgcgc
    10621 cgccgagcgc tcggccgccg agggaactgc agcgtccggc gccgatcgaa gcaaggtatc
    10681 gctggaggat ttcttccacc gggcgcaggc cgagggcaac aaggaattgc tgctggtcat
    10741 caaggcagat ggacagggtt cgattgagcc actggtcagc tcgttggaaa agctcgatca
    10801 gggcggcgcg agggtcaagg tgctgcacac cggaacaggc cgcgtgagcg aatcggacgt
    10861 gtcgctggcc gtcgcctcag acgccatcgt catcgccttc aacgtcgaag tggacgagtc
    10921 ggcgcgccgg ctggctgact cggaaggcat cgacgtccgc aagtacaacg tcatttacaa
    10981 gctggtggag gacatcgaca aggcgctgca gggcatgctc gagccggtct acgaagataa
    11041 ggttgtggcc cgcgccgagg tgcgccaggt attcaagatc aggaatgtcg gcttcatcgc
    11101 cggctgcctg gtgcgcgagg gcgcggc
//
LOCUS       DBNHXB010000089         9586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1358544, whole genome shotgun sequence.
ACCESSION   DBNHXB010000089 DBNHXB010000000
VERSION     DBNHXB010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9586
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1358544"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="AC162G_03400"
     CDS             complement(<1..954)
                     /locus_tag="AC162G_03400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5074894.1"
                     /translation="MIRQSGTSSSIGRNDMFSFEPSEEQKMLVDAVLKFALKDIREAA
                     PEADEKCQLPQTLIGKGWDLGLLQSSIPEEYGGFGARSAVTLALAAEELAYGDLATAM
                     AILAPNLFALPILIAGSQEQKQTHLPKFCEGGYLPATAALMEPRYDFQPGALKTTARD
                     DGDGWVLDGEKCYVPLAAEAEHLLVYAALDGQTQGFIVRKDTPGLSIGEREQNMGIRA
                     LPTYTLHLSNCKVACADRLGGEAGHDFAPIINASRAALAAMAVGVSRGAYEYALKYAK
                     EREAFGEPIAQRQSIAFMLAEMATEIEATRLMAWEAAWLIDQ"
     gene            complement(951..2036)
                     /locus_tag="AC162G_03405"
     CDS             complement(951..2036)
                     /locus_tag="AC162G_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HIR5074895.1"
                     /translation="MKAAVCYEFGKPLVIEEIDIDPPQPGEVKVKVAATAICHSDVHL
                     VRGEWGGKLPVVAGHESAGIIAEVGQGVTALRPGDYVVVSLLRSCGRCSDCTSGAPHL
                     CSGSFALDSQSRLHSKHGQTIHHGIKTASFAEYTIVDQSQCVKIPDDVRPESAALLAC
                     GVITGMGAVTNTANVTPGSRVAVIGVGGVGVNSIQGAVLREARMIIAIDQVEDKLAVA
                     RTFGATHTVNASTEDPVKAVRALTDGAGVDYAFVTVGNALAINQAFKMVRRGGSVVAI
                     GLPEASATVDLRVHSLVSGERRILGSFMGSTRLSVDVPKLVELYQQGRLKLDELISNC
                     YSLEQINEAIAAMEHGAALRNVIVFPQ"
     gene            2348..3892
                     /locus_tag="AC162G_03410"
     CDS             2348..3892
                     /locus_tag="AC162G_03410"
                     /inference="COORDINATES: protein motif:HMM:NF025774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HIR5074896.1"
                     /translation="MPLNQHRCIFYECTDDLWNLCVPLIHESAHTGFKWLYIADEHDV
                     RDVLSAIKSHLQPLPRGEAVNADSLSLLETSISFNTIISKLRAHAQLALEEGFHGLFL
                     LTEMTWSLRTSFGVAYLSEFEGRLHELLSSTSMRAGCLYNRRVFPETMILEALRTHPG
                     VYAADGLHRNPHFVPPTALLSGDVRIQLQHWLRAISPTLADAWDQQNEASEPSHASPP
                     ISHPASTISKVPIYSLESPAPLVASNTDQHRWKIHCLGELKVYRQDGTPVRWNMMHGA
                     TLKTKTLFAYLLHCGQKGATAEELADFLWPEANSTEQGLNRLYHTVYCLRMALSPALT
                     SSRDSPFVLNDDQRYYLSLPEGTWIDIPVFEQLCYKAERLLRENKDEESLICHRAAEN
                     LYRGALLADIPAQYAENVDKDWCWSRRYWLQEMYLKMLTYIAGTYRRRNDIPRALVYC
                     EKVLTIDACSERAHQEMMRNFHASGRRDALDRQYRLCIQALERYDGRAPSPVTQALYQ
                     SLIATV"
     gene            4176..5108
                     /locus_tag="AC162G_03415"
     CDS             4176..5108
                     /locus_tag="AC162G_03415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR5074897.1"
                     /translation="MKLGVFLPVSGRAAVPEVLMDAARQAETLGYDSVWAAERLVNPW
                     VMKTPYPYKDSQQWFVPPDSPFLEPLTCLAFLAGCTERVALGISVAVLPYRHPLYIAR
                     VATSIDTLSKGRLILGVGIGWMVEEFEALGVPFKKRAGMANEQLQIFNLLWKEERPRF
                     EGRYYRFDEVAVSPKPVQKPRFPIWTGGESEAAQRRAATYGDAWFSYFVKITPQDLAA
                     RFANVRKLAAEAGRDPAEVHFCCCRPIDITSAPVPQEEDTLKGTPEQLIEALKRFKEI
                     GVEHMALQFMVGRWPERKVKIERFAKEVMPALQA"
     gene            5175..5945
                     /locus_tag="AC162G_03420"
     CDS             5175..5945
                     /locus_tag="AC162G_03420"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5074898.1"
                     /translation="MTGRVALITGGSKGIGRAVALAMAEAGADVAICARKMADLEPVA
                     DAVRATGRRAFATVCDVTDAGQVNQLPATVGEALGSIDILVNNAGASESHKFVGHPDN
                     LWARMLEINLTSVYRVCKAIVPGMIERKWGRIITIASISSKVGARYVAAYTASKHGVL
                     GLMRALAVELNPYHITVNAICPGYVDTPMTDANVANMMARTSKSEAEVRKFLEATNPQ
                     SRLISPEEVAAVAVMLAGEGARGITAQAINVDGGALVY"
     gene            6049..6510
                     /locus_tag="AC162G_03425"
     CDS             6049..6510
                     /locus_tag="AC162G_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003961116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5074899.1"
                     /translation="MAITTQTMTDEQRKSVALEYLKAFDNAGVTSSGGSILDLFATDA
                     QVYFPKWGLAAGRDQISKLFSDVGGTLKSIVHHYSHFNWIFSGSDLVVCEGTSHGEHR
                     DGPWRAGVPDWGAGRWCDVFEIRDFKIQRCFIYLDPDYAGKDTARYPWLNP"
     gene            6519..8096
                     /locus_tag="AC162G_03430"
     CDS             6519..8096
                     /locus_tag="AC162G_03430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HIR5074900.1"
                     /translation="MMRKAAEVPLYYNAVDILERNLTKRADKVALYSPDRAMTFRQVS
                     NEANQVGNALKKLGVRLGDFVGILSPDGPEWVTSFFGTLKIGAIAVGMNTLLTRQEYA
                     YILRDCRARVLIVHESLLPIVEDIRDEQRFLERVVVIGYPARTGDLAYRDWISSEPTE
                     FDAGPTHRDDLCTLNYSSGTTGEPKGIPHAHKDLPLSAQLYGVDALGLCEDDRTFAVA
                     KLFFTYGLGNNLIYPWYTGASVVLSPAPSRIAANVLEAIDRFKPTVLNSVPTNYAAML
                     AVEGFTEKYDLSSLRMGISAGEALPAPIWHAWKKQTGVEIIEGIGLVENFALFLTNRP
                     GASRPGSTGKPVEGFEIKIVDDDDNPVPPGEVGNLLVKGETASLSYLHQYEKSRRTFR
                     GEWLFTGDKFYVDEDGYYHHVGRTDDMLKVGGIWVSPTEVESVLISHPAVLEVAVVGH
                     PDQADLIKPKAFVALNKGYQPSQELAQELIEHCKEVMSAYKRPRWIEFVDELPKTATG
                     KIQRAKLRSQHFDYFIL"
     gene            8117..8515
                     /locus_tag="AC162G_03435"
     CDS             8117..8515
                     /locus_tag="AC162G_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR5074901.1"
                     /translation="MDTVLLIGRIIVGGYYLFNALNHFNLFGQTAALSGWATFKGVPA
                     SRALVLFAGLLLAIAGLTFLTGFMPVIGVIALGIFFVPVTPIMHNFWVETDPLAKWNQ
                     IHQFIKNVGLFGSALMFLAIPQPWPFSLGG"
     gene            8547..9515
                     /locus_tag="AC162G_03440"
                     /pseudo
     CDS             8547..9515
                     /locus_tag="AC162G_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
BASE COUNT         1940 a         2860 c         2817 g         1969 t
ORIGIN      
        1 ctggtcgatc agccaggccg cttcccaggc catcagccgc gtcgcttcga tctcggttgc
       61 catctccgcc aacatgaacg caatcgattg acgttgtgcg atcggctcgc cgaaggcttc
      121 gcgctccttg gcgtacttga gcgcgtattc atacgcgcca cgactcacgc ccacggccat
      181 cgccgccagt gccgctcgcg aggcgttgat gatcggtgca aagtcgtgcc cggcttcccc
      241 gcccagccga tccgcgcagg ccaccttgca gttcgacaga tggagcgtgt aggtcggcag
      301 cgcgcggatg cccatgttct gctcgcgttc gccgatcgac aggccaggcg tatccttgcg
      361 gacgatgaaa ccttgcgttt gcccgtcaag cgcggcgtat accagcaggt gttcggcctc
      421 ggcggccagc ggcacgtaac acttctcgcc gtcgagcacc cagccgtcgc cgtcgtcgcg
      481 cgccgtcgtc ttcagcgcgc ccggctggaa gtcgtagcgc ggttccatca gtgcggcggt
      541 ggccggcagg tagccacctt cgcagaactt cggcaggtgg gtctgctttt gctcttgact
      601 gccggcgatc aggatcggca gcgcgaacag gttcggcgcg aggatcgcca tcgccgtggc
      661 gagatcgccg taggccagct cttcggcggc cagcgccagc gtcactgccg agcgcgcgcc
      721 gaacccgccg tattcctcgg ggatcgacga ttgcagcagg ccgagatccc aacccttgcc
      781 gatcaacgtt tggggcaact ggcacttctc gtccgcctcg ggcgcggctt cgcggatgtc
      841 tttcaacgcg aacttgagca ccgcatcgac taacattttc tgttcttcag atggctcgaa
      901 cgaaaacata tcgtttctcc caatcgatga tgatgtgccg ctctggcgga tcattgcgga
      961 aacacgatga cgttgcgcaa agccgctccg tgttccatcg ctgcgatggc ctcgttaatc
     1021 tgttccaggg aatagcaatt tgagatcaac tcgtcgagct tgagcctgcc ctgttgataa
     1081 agttcgacca gcttgggcac gtctacgctc aatcgggtag agcccatgaa gctgcctagg
     1141 atgcggcgct cgccgctgac gagagagtgc accctgagat cgacggtcgc cgaagcctcc
     1201 ggcaagccga tcgcgacgac gctgccgccg cgccggacca ttttgaacgc ctggttaatg
     1261 gcgagcgcgt tacctacggt gacaaatgcg tagtccactc ccgctccatc ggtcagtgcg
     1321 cggacggctt tcaccgggtc ttcagtgctg gcgtttaccg tatgcgttgc cccaaatgtg
     1381 cgcgctactg ccagcttatc ttccacctga tcgatagcga taatcatgcg cgcttccctg
     1441 agaactgcgc cctggatcga attcacgccc acgccaccca cgccgatgac cgcgacacga
     1501 ctacctggcg tgacgttggc cgtgttggtg accgctccca tgcccgtgat gacgccacac
     1561 gctagcaggg cagcactctc cgggcggaca tcgtcgggga ttttgacgca ttggctctga
     1621 tcgacaatcg tgtactctgc aaaagacgcc gttttgatac cgtgatgaat cgtctggccg
     1681 tgtttgctgt gcagacgact ctgtgaatcg agcgcaaaag agccgctgca caggtgcggc
     1741 gcgccgctcg tgcaatccga acagcgacca caagagcgca gcaacgacac gaccacgtaa
     1801 tcgcctggcc ggagagcggt gacgccctgg cccacttcgg caatgatccc cgcggactcg
     1861 tgaccggcaa ccacgggtaa tttgccaccc cattcccccc gcacgagatg cacgtccgag
     1921 tgacagatag ccgtggcggc caccttgact ttcacctcgc cgggctgagg cggatcgatg
     1981 tcgatctcct ctatgaccag cggcttgccg aattcatagc agacggctgc cttcaaaatt
     2041 cattccctcc gtatctgagc gatataaccc gacagatccc gatgaggctt tcgtgcaccg
     2101 tgctgtccaa ggatactcgc atcagcgctt atggtcaagc acgatacggc attcgttcga
     2161 atctacaccc tgcctttgaa gacatcattc tggagtgtct aagcggtgaa catctgacct
     2221 ttggggcagg agaagctcac aggcgcattt ggggttataa tccgtgcagg tttgctgatg
     2281 acgcttcgtc tattgaatgc agatggctgc tgttcgcgcc gtcgaagctt gggattagtt
     2341 ctcagcgatg ccgctcaacc agcaccgatg tattttctac gaatgcacgg atgatctgtg
     2401 gaacctatgc gttcccctga ttcacgaaag tgcgcatacg ggtttcaaat ggttgtacat
     2461 cgccgacgaa catgacgtcc gcgatgtcct atcggccatt aagagccacc ttcaacctct
     2521 gccccgcggc gaggcggtca acgccgactc gctgagcctg ctcgagacct ccatctcgtt
     2581 caacaccatc atcagcaaac tgagagcgca cgcccaatta gccctagaag aaggtttcca
     2641 cggtttgttc ctcctcacgg agatgacatg gtccttgcgc acgtcgtttg gtgtagctta
     2701 cctgagcgaa tttgaaggcc ggttgcacga gttgttgagc agcacctcta tgcgtgctgg
     2761 gtgtttgtac aaccgtcgcg ttttcccaga gaccatgatc cttgaggcgc tgcggacgca
     2821 tccaggcgtc tacgccgccg atgggctgca ccgtaacccg catttcgtgc cgccgacagc
     2881 cttgttgagc ggggatgtcc gcatacagtt gcagcactgg ctgagggcga tcagtccgac
     2941 tttagccgat gcctgggatc aacagaacga ggcatccgaa ccgagtcatg cgtcgccgcc
     3001 aatatctcat cctgcgagca cgatctccaa agtgcccatc tacagcctcg aatcgccagc
     3061 cccacttgtg gcgtccaata ccgatcagca tcgctggaaa attcactgtt tgggggagtt
     3121 gaaggtttat cgtcaggatg gcacacccgt gcggtggaat atgatgcatg gcgcgacgct
     3181 caagaccaaa acgctgttcg cctatcttct acattgcgga cagaaaggcg ccacggcgga
     3241 ggaacttgcc gacttcctct ggccggaggc gaactcgacg gaacagggtc tcaatcggct
     3301 ctatcacacc gtctactgtt tacgcatggc actcagcccg gcgctgacct ccagccgcga
     3361 ctcaccgttc gtcctcaatg acgaccagcg gtattacctg tctttgccag agggtacgtg
     3421 gattgatatt ccggtgttcg agcagctctg ttacaaggcg gagagattgc tgcgcgagaa
     3481 caaggacgaa gagtcgctca tctgccatcg ggcggcggaa aacctttacc ggggtgcttt
     3541 gttggcagat attccggcgc agtacgctga gaacgtggac aaggattggt gctggagccg
     3601 ccgctactgg ctgcaagaga tgtacttgaa gatgctgact tacattgcgg gcacttatcg
     3661 tcgtcgcaat gacattccgc gcgccctggt ctactgcgag aaagtattga cgattgatgc
     3721 ctgttccgaa cgcgctcatc aggagatgat gcgtaacttt cacgccagcg gtcggcgcga
     3781 tgccttggat cggcagtacc gtttgtgtat tcaggcacta gagcgatacg atggacgcgc
     3841 accatcgccg gttacgcagg cgctctatca atcactcatt gccacagtat gaactgctgc
     3901 tctccagttc ggccttccac tcgacatcca tcccaatccc gtccgctcgt ctgccgcgca
     3961 cggctaaaga tagactaaag gtcctcgacg taagtttatt acaattagcc ttcaccttct
     4021 gttccctcct ccggcgtcaa gggtattgtc ctgctcgatg gcattgtgac accggcaacg
     4081 atgactgggt tgccggacca agatgaatat ttcggccgag gtctttcaag cggctgatac
     4141 ggcggcacct actaagctca aggaggaatg tcaacatgaa actgggtgtc tttctccctg
     4201 tctccggccg ggcggcagtc ccagaggtcc tgatggatgc tgcccgccag gccgagactc
     4261 tgggttacga ttcggtctgg gcggcggaac gtctcgtcaa tccctgggtc atgaaaaccc
     4321 cttacccgta caaagacagc caacaatggt tcgtcccccc cgatagtcct ttcttggagc
     4381 ctctcacctg cctggccttc ctggccggtt gcaccgagag agtcgcgctc ggtatcagtg
     4441 tcgccgtgtt gccctatcgc cacccacttt acatcgccag agtagccacc tctatcgata
     4501 ccctctcgaa aggacgactg atcctggggg tggggatcgg ctggatggtc gaagagttcg
     4561 aggcgttggg cgtgcccttc aagaagcggg cagggatggc caatgagcag ctccagattt
     4621 tcaacctgct ctggaaggaa gagcgcccac gcttcgaggg gcgctattat cgtttcgacg
     4681 aggtggcggt cagccccaag ccggttcaga aacctcgctt cccaatctgg acaggtggag
     4741 aaagcgaagc cgcccagcgg cgggcggcca cgtatggcga cgcctggttc tcctactttg
     4801 tgaagattac gccccaggat ttagctgcgc gttttgccaa tgtccgaaaa ttagccgccg
     4861 aggcaggccg ggacccggct gaggttcatt tctgttgttg ccgccccatt gatatcacct
     4921 cggcaccagt gcctcaggag gaagatactc tgaaaggtac gcctgaacag ctcattgaag
     4981 cgctgaagcg gttcaaagag atcggcgtag aacacatggc gcttcaattc atggtaggcc
     5041 gttggcctga gcggaaagtg aagatcgagc ggtttgccaa ggaggttatg cctgcacttc
     5101 aggcttagca cgtcatggcg ccgaccaaca cagcagggtg agaaagaggt agtcgtcctg
     5161 gcatgacgaa ctctttgaca ggccgagtag cgctcatcac gggcggcagc aagggcatcg
     5221 gtcgcgccgt ggcgctggcg atggcggaag cgggcgcgga cgtagcaatc tgtgcgcgaa
     5281 agatggcaga cctggagccg gttgcggacg cggtccgggc gactgggcgc cgcgccttcg
     5341 ccacagtgtg tgatgtgaca gacgccggac aggtcaacca actgcccgcc acggtgggcg
     5401 aagcattggg gtcgattgat attctggtga acaacgccgg cgcttccgag agtcacaagt
     5461 tcgtgggcca tcctgacaat ctctgggcgc gcatgctgga gatcaatctg acgagcgtat
     5521 accgggtctg caaggcgatc gtgccaggca tgatcgaacg caaatggggg cgcatcatca
     5581 ccatcgcttc catttctagc aaggtcgggg cgcgctacgt ggccgcgtac accgcttcca
     5641 agcatggtgt gctgggattg atgcgcgccc tcgccgttga actcaacccc taccacatca
     5701 cggtcaacgc catctgtccc ggttacgtgg atacgcccat gaccgacgcg aacgtcgcca
     5761 acatgatggc gcggacgagc aaatccgaag ccgaggtgcg caaattcctg gaagccacca
     5821 acccgcagag tcgcctgatt tcgccggaag aggtcgccgc cgtggccgtg atgctggccg
     5881 gtgagggggc gcgcggcatc actgcgcagg cgatcaacgt ggacgggggg gcgctcgtgt
     5941 attgagaccg gaagggaggt gattgtcaga agaactgcca gaagcgaata ggttgagtca
     6001 caggcaccac tctcgtcaac cttattctcg atgcatgagg aggcaaccat ggcaatcacc
     6061 acgcagacca tgaccgacga gcagcgcaaa tcggtcgcac tggaatatct caaggcattt
     6121 gacaatgctg gcgtcacatc gtcgggcggc tcaatcctcg atctgtttgc taccgatgcc
     6181 caggtctatt tcccgaagtg gggcctggca gccggccggg atcagattag caaactcttc
     6241 agcgatgtcg gaggtacgct caagtcaatc gttcatcatt actcacactt caattggatc
     6301 ttctcgggca gcgacctggt ggtctgcgag ggcacaagtc acggtgagca tcgtgatgga
     6361 ccctggcgtg ctggtgtacc ggactggggg gctgggcgct ggtgcgacgt tttcgagatt
     6421 cgcgatttca aaatccagcg ctgctttatc tatctggatc ccgactacgc cggcaaggac
     6481 actgcgcgtt acccctggct gaatccttga tcgacaggat gatgagaaaa gccgcagagg
     6541 ttccccttta ctacaacgcc gtcgacatcc tggagcgcaa cctgaccaaa cgcgcggaca
     6601 aagttgcctt gtacagcccg gatcgggcaa tgaccttccg gcaggtgtcg aacgaggcga
     6661 accaggtcgg gaatgcgctc aagaagctgg gagttcgcct gggggacttc gtcggcatcc
     6721 tgtcgcccga tggcccggag tgggtcacct cgtttttcgg cacgctgaaa atcggcgcga
     6781 ttgcggtagg catgaatacc ctgttgacgc ggcaggagta cgcctacata ctgcgcgact
     6841 gccgcgcccg ggtattgatc gtccacgaat ctctgctgcc aatcgttgag gacatccgtg
     6901 acgagcagcg atttctggaa cgcgttgttg tgatcggcta cccggctcgg acaggcgacc
     6961 tggcctatcg cgactggatc tcctccgaac cgaccgaatt cgatgctggc ccgacccatc
     7021 gcgatgatct gtgcacgctg aattattcca gcgggacgac gggcgagcct aagggaatcc
     7081 cgcacgcaca caaagatctg ccgctcagtg cccagttgta cggtgtcgat gcgctgggcc
     7141 tatgcgagga tgaccgcacc ttcgccgttg ccaaactatt cttcacatat ggcctgggca
     7201 acaatctgat ctacccctgg tacaccggcg caagcgtcgt gctgtcccct gcgccgtcgc
     7261 gcatcgccgc caacgtgctg gaggcgatcg accgcttcaa gccgaccgtc ctgaacagtg
     7321 tgccgaccaa ctacgcagcc atgttggccg tcgagggttt caccgagaaa tacgatttgt
     7381 cctcgctgcg catgggcatc tctgccgggg aagcgttgcc ggcgcctatc tggcatgcct
     7441 ggaagaagca gactggcgtg gaaatcattg aagggatcgg attggtggag aacttcgctc
     7501 tcttcctgac gaaccgcccc ggcgctagcc ggcctggcag caccggtaag ccagtggagg
     7561 gttttgaaat caaaatcgtg gacgacgacg acaatccggt gccgcccggt gaggtgggca
     7621 acctgctcgt caagggcgag acggcctccc tatcctatct gcaccaatac gagaagagcc
     7681 gccggacgtt ccggggtgag tggctcttta ccggcgacaa attctacgtg gacgaggatg
     7741 gctattacca tcacgtcggg cgcacggacg acatgctgaa ggtggggggc atatgggtct
     7801 caccgacgga ggtcgaaagc gtcttgatca gtcacccggc ggtgttagaa gtcgcggtgg
     7861 ttgggcaccc cgatcaggcc gacttgatca aaccgaaagc gttcgtcgcc ttgaacaagg
     7921 gctaccagcc ttcgcaggaa ttggcccaag agctgatcga acattgcaag gaggtgatgt
     7981 cagcctacaa gcgcccgcgc tggattgagt tcgtggatga gctgcccaag acggctaccg
     8041 gcaagatcca gcgtgccaag ttgcgcagcc agcactttga ctacttcatc ctgtgataaa
     8101 tctaaggagg tccacaatgg acaccgtatt actgatcggg cgcatcatcg tgggcgggta
     8161 ctacctgttc aacgccctca accacttcaa cctctttggc cagacggcgg ccctgagcgg
     8221 ctgggccact ttcaagggcg tgcccgcctc tagggcgctg gtgctgtttg cgggtttgct
     8281 tctagccatc gccgggctga cctttctgac gggtttcatg ccggttatcg gggtcattgc
     8341 gctgggcata ttctttgtgc cggtaacccc tatcatgcat aacttctggg tcgagacaga
     8401 cccgctcgcg aagtggaacc agattcatca gttcatcaag aatgtcgggc tgttcggctc
     8461 ggcgctgatg ttcctggcca ttccacagcc ctggcccttc agcctgggcg ggtagaagcc
     8521 catctgctca caaccgctgg agcgagatga ccaggcacat cttgccactg gagcaaatca
     8581 ccaaagatga tgttcaacgc tgcgggggca aagcgaccag cctgggcgag ttgacgcggg
     8641 ccggcatctc cgtgccgccc ggcttttgca tcgccgccgc ggcgttgtcg tatttggcga
     8701 cctccaactc aatcggcgag cgcatcgccg atatggcggc caggctcaac ttcgaggact
     8761 acgccgatgt cgaaaagcgt acggccgaga tccgtgcact catcacgtcg gccaccatcc
     8821 ccgccgacct cgagcaggac atcctccagc actatcggac gctgaccagc ggcagcaaca
     8881 agttcgtcgc cgtgcgctcg tcggtcgcgg tgaaggacag cccgatctct tcctttccag
     8941 ggatgatgga tacctaccac tatgttctgg gtgaaggtca ggtactggag aagatccgtg
     9001 agtgctgggc gtcgctgtgg acggcgcgcg cggcgttcgc ccgccatcag aagaacatcg
     9061 cgcacgagcg aggcttgatc gctgccgtca ttcagctcat ggtgaacgcc gacgtcgccg
     9121 gagtgctatt tacggccaac ccgatctcgc gggccaccaa tgaactggtg atcgaggcca
     9181 actgggggct gggcgagtcg gtcgtttccg gccggtccat gaacgacttc tacgtcctcg
     9241 acaaggacaa cctggccgtc aaacaggaga agattgcccg aaagaccatg atggtgacca
     9301 tggactacga tcggggctct ggccgcaggg agcagcccgt gccgccggaa cgggccacag
     9361 cgcccgcctt gtctgctgcg cagttggttg agtgatgaga gatcggccgg catatcgagc
     9421 gccacttcga tttcaacgcc gacatcgaat gggcctatca gaacggcgcc ctctacatct
     9481 tgcagacacg ccggatccgc taccagggtg aataatcacg aacagaccca aactaaggag
     9541 gatatcccag gccacagcga accgttgacg ttcacaatca ttggta
//
LOCUS       DBNHXB010000090        10940 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2365123, whole genome shotgun sequence.
ACCESSION   DBNHXB010000090 DBNHXB010000000
VERSION     DBNHXB010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10940)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10940)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10940
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2365123"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..304)
                     /gene="yidD"
                     /locus_tag="AC162G_03445"
     CDS             complement(95..304)
                     /gene="yidD"
                     /locus_tag="AC162G_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR5074902.1"
                     /translation="MKYLALGVIRLWQSTISQALPPSCRFTPSCSQYGYEAIAKYGIL
                     KGGWLTVKRISRCHPLNPGGYDPVP"
     gene            complement(301..666)
                     /gene="rnpA"
                     /locus_tag="AC162G_03450"
     CDS             complement(301..666)
                     /gene="rnpA"
                     /locus_tag="AC162G_03450"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR5074903.1"
                     /translation="MRVQRLTRRADFERVRGEGQTHGTPLFVVVAARRDGGPPRVGVA
                     AGRRVGGAVQRNRAKRLLREGIRPLYASIAPGWDILLMARGAVLEASSTQITARLDQV
                     LRKLSVIAPQDHTIDRDDP"
     gene            complement(719..847)
                     /gene="rpmH"
                     /locus_tag="AC162G_03455"
                     /pseudo
     CDS             complement(719..847)
                     /gene="rpmH"
                     /locus_tag="AC162G_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
     gene            1061..1852
                     /locus_tag="AC162G_03460"
     CDS             1061..1852
                     /locus_tag="AC162G_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR5074904.1"
                     /translation="MARVIAIANQKGGVGKTTTSSNLGMALAERGRRTLLVDLDPQAA
                     LSAGFGIDVYSLEKSIYDALLHDHETPLASVRYNLRPNLDIIPANIDLAAAEIELVVM
                     MGRESVLREALEPIANEYEYILIDTPPSLGLLTVNALVVADELIVPLQCEYLAMRGVR
                     VLLDTIWKVKRKLNPRLDLLGILPTMYRSRSTHSKEVLEEVRNIFGDKVFDIVIGNSV
                     RFAEAPVAQQTILEYEPRHAGAKAYRYLAEVVDGKKETITEGQGG"
     gene            1812..3215
                     /locus_tag="AC162G_03465"
     CDS             1812..3215
                     /locus_tag="AC162G_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074905.1"
                     /translation="MGRKKPSLKGRGVDILLGGSADEQPTEPAAQPVAVTAEPGPTTL
                     PGSVEAAPGPGVEAGPGAPMPNPFAEPAPALPVYAAKPTTEAGVADFNPFAEPASVSS
                     SAAARPIAEAAATMPNPFAEPLPAAAAAPSAAEAASIALPSDPTLFKPAQYGGIAMSI
                     EVMSGSGEYQPPTGVTQTGMKAVTLTSTGEQVSAEEVLQRIGSERIQGLSMRVDDLYA
                     QVAGGAMANAEQAGEALSNLRTARDKELEDPRQFDEAEYLVNLVQYQINHSASIQQVR
                     NWSYTWGVVVFIYGVFWLLVFGAGIAVSALGLVDQWLTDLALDEQTITTASALFLSVT
                     SGGLGAVMGLLYSLNKHAAIQQDFDRQFLLWYYVQPFLGMLMGVIVNLFLFAGVFLVD
                     ATGPAIQAIGALLAIATAFRQNYVYAWLESLLRGFERRASAAEERREAEAPKPEVQPT
                     TPTAPAGEAQGSPAGVG"
     gene            complement(3299..4591)
                     /gene="murA"
                     /locus_tag="AC162G_03470"
     CDS             complement(3299..4591)
                     /gene="murA"
                     /locus_tag="AC162G_03470"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR5074906.1"
                     /translation="METFVIEGGYPIGGAVVPSGNKNAALPLIAAALLTEEPVTLYNV
                     PRIRDVGAMLKLVCDLGAEVSERDDGHTVCIRAANLRKTDLNADLCRDIRASILLAGP
                     MLARLRHVELPPPGGDVIGRRRLDTHFLAFVALGAQVEANARFRLRADKLTGADILLD
                     EASVTGTENTIMVACLADGATILRNAASEPHVQELCHLLSRMGARIEGIGSNTLTIHG
                     VRSLHGTEATIGPDYLEVGSYIGLAAMTGGELLVRDAAPEHMGMIRLMLGRLGVRFEA
                     RGPDIFVARGQSLTVVPDIGDAIPKIDDGPWPAFPADLMSTAIVVASQAAGTVLFHEK
                     MFESRLYFVDRLIAMGARIILCDPHRCIVQGPSWLHPEPGGVASPDIRAGMALVLAAL
                     CAQGTSVIRNIGQIDRGYERIEEKLRALGAKIERVKNG"
     gene            4826..5788
                     /locus_tag="AC162G_03475"
     CDS             4826..5788
                     /locus_tag="AC162G_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074907.1"
                     /translation="MAQISGKQPLGILTALSTGFDAVTRQLWLLALPAALDVFLWLGP
                     RLKTPGVSRLFMFEIPAGLDANTRLFAQDFQDSLRHVLENANWFVWLLRPALLGVPGL
                     SDALGEPPPGVTPTVWPLADIGALVAAVIVLVVLGVGLSGLYWSLVARQARDGRIDWW
                     AAWGRMAVVWPRMLALALLLIGMVTAIWTPALILSALLGSALGLSGALLVMVSFALLV
                     WLLFYTAFSVHGIVLYNQRVLDAVRASVDLCRAHFWKTLGLLAALVAIEWGMGLIWNQ
                     VPSGSWLWAVSIAGNAFVVAGLSMASMMYYMEHVPVPRSTLVPQ"
     gene            5840..7813
                     /gene="gyrB"
                     /locus_tag="AC162G_03480"
     CDS             5840..7813
                     /gene="gyrB"
                     /locus_tag="AC162G_03480"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR5074908.1"
                     /translation="MSNEKPSTYDASQIQVLEGLEAVRRRPGMYIGSTDIRGLHHLVY
                     EVVDNAIDEALAGQCDQIYVTIHKDLSVSVQDNGRGIPVGPHPTQKDASGKPMDALEV
                     VMTILHAGGKFGGEGYKVASGLHGVGVSAVNALSGWMETTVRRDGKIYRQRYERGRPT
                     GKVEQAGRILKDDPYPHGTLQRFTPDPQLFKEGIDYKFETLAQRFREMAFLTKGLTIT
                     FCDERDGHEMSFYFEGGVRSFVRYLNKNRAVLHDPVHLEKQVNGTAVEVAIQYTDGYN
                     ESVFAFANNINTVDGGTHLTGFRSALTRTINDYARKAGLLKDSDPNFSGEDVREGLTA
                     IVSVKLQDPQFESQTKGKLGNAEVKGHVESVVSEAFGEFLDESPREAKQIVQKCLTSS
                     RAREAARQARDLVIRKSALESLTLPGKLADCSERDPSKCEIYIVEGDSAGGSAKQGRD
                     RHFQAILPLRGKILNTERARMDKILANNEVKALISALGCGIGDQFDLSNLRYHRVILM
                     SDADVDGSHIRTLLLTFFFRYMEQLIENGHLYIAQPPLYRIEVKKGAKDVVYAYSDPE
                     REGIYVQLRKKGLDPADDKQVVTQRYKGLGEMSAEQLWETTMNPENRTLLQVTIEDAA
                     AADKTFDMLMGDEVPPRKRFIQTHAKEVRNLDI"
     gene            7859..8431
                     /locus_tag="AC162G_03485"
     CDS             7859..8431
                     /locus_tag="AC162G_03485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015172489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR5074909.1"
                     /translation="MTTERLSEALVFAAHLHARQTRKGTHIPYIAHLMAVAGLVLENG
                     GDEDEAIAALLHDAVEDQGGAATRENIRRRFGGRVVEIVDGCTDSDRVPKPPWRARKE
                     AFLARLPRASSSVRLVSAADKLHNARTILADYRAMGDVVWKRFTGGKSGTLWYYRALV
                     DAFCAAGTSSLVEELARVVAELDQVERQTG"
     gene            8633..8791
                     /locus_tag="AC162G_03490"
     CDS             8633..8791
                     /locus_tag="AC162G_03490"
                     /inference="COORDINATES: protein motif:HMM:NF042615.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6800 family protein"
                     /protein_id="HIR5074910.1"
                     /translation="MKVRDREIRRRRARRRKTWRLKARMAATTDSRAKARLAEKIRRV
                     NPYVQDAK"
     gene            8949..9881
                     /locus_tag="AC162G_03495"
     CDS             8949..9881
                     /locus_tag="AC162G_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5074911.1"
                     /translation="MNSSQAIETANLRKVYNGKVAVADLSLSVPRGEVFGFLGPNGAG
                     KTTAIKMLLGLAAPTSGTGRLLGRPIGDPAARARAGFLPEHFRFHDWLTGAEFLHLHG
                     QLYRMREVDRRQRVPELLARVGLAEHGDKRLRGYSKGMLQRIGLAQALLNRPEIVFLD
                     EPTSGLDPVGRILVRDIIRDLREQGTTVFLNSHLLSEVEITCDRVIFIKHGAVVRSVS
                     LGEMGQGALPVEIRIGALPAGAQADLLSGLSRWGRDARVEDGRLHVIVDDEASLPEIA
                     RWLVGQGIDLYGLIPQRMSLEDMFLQIVGTDGGL"
     gene            9883..10731
                     /locus_tag="AC162G_03500"
     CDS             9883..10731
                     /locus_tag="AC162G_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5074912.1"
                     /translation="MAARIITGLTIREARRRKVLWGLLILSALFLGLYTLGLNFVYTE
                     IMSHGGPPRGASRLFSLDDVWNFFLTAALYGANFLLVMMAVLISVDTLAGEISSGTIQ
                     SIAVKPLRRHEILLGKWLGFVVMLAGYTLLLAGGVIAITALITGYALPAWLPTLVLMY
                     LEALVLLSVSIVGGTRLSTLATGVLGFGLFGVAFIGGWIEQIGSFILVQSEAAVSIGR
                     LASFAMPSEALWRYTLSEVTTSRNPFSFIFGGTSAPDAGVVVYAIAYAVALLALAVLS
                     FRRRDL"
BASE COUNT         1917 a         3373 c         3612 g         2038 t
ORIGIN      
        1 gccaccgcag gcggtgataa gcccggcaca gaatacgatg acagcgatgg acagccatcg
       61 ccagggaagg tgatttgtca actacgctcc ttgctcaggg gacggggtcg tatccgccgg
      121 gattcaaagg gtggcagcgc gatatgcgct tcaccgtcag ccagccgcct ttcaagatgc
      181 cgtatttcgc gatcgcttcg tagccgtatt gagaacagga cggggtgaaa cgacaacttg
      241 gcggcagcgc ctgcgaaatc gtcgactgcc acaatcgaat cacgcccagc gccagatatt
      301 tcatgggtcg tctctgtcga tcgtgtggtc ctgtggcgcg atgacactca gttttcgcaa
      361 gacctgatcc agcctggcgg taatctgtgt cgagctggcc tccaggacgg cgccccgcgc
      421 catcaggagg atgtcccacc ccggtgcaat agatgcgtac aacggacgta tcccctcgcg
      481 caggagacgt tttgcgcggt tgcgctgcac tgcgcccccg acgcgcctgc cggctgccac
      541 acccacacgg ggcggcccac cgtcccgccg cgccgccacg accacaaaca acggtgtgcc
      601 gtgcgtctga ccctcgccgc ggacgcgctc gaagtccgcc cggcgggtca atcgctgcac
      661 gcgcatcgac ggctcagttc ttccagtcga tgcgcttggc gagccggggc ttgagcgcga
      721 tgttgcggcg ccccttgcgc cggcgtgctt tgagcacgtt gcgcccgccc gatgtggcca
      781 tgcgcgcgcg aaagccgtgc gtgcgcgcgc gatggcgctt cttgggttga taggttcgta
      841 cggtcataga ctgtcctctt tacacagcgc aagggcggca ggcgccctta cgacaagccg
      901 actgtggtgt ccgactattg tgcgacacaa attatacctg atgcattccg cgcggtcaaa
      961 tgctgcgggt gacattgcac gccatcacag gcatttgctt ttggtgtggc acctgggcta
     1021 taatacgctt gcctcgacga gttcgagaca ggagatgtgc atggcgcggg tgatcgcgat
     1081 tgccaaccag aagggcggcg tcggcaagac gacgacgtcg agcaatctgg gtatggcgct
     1141 cgccgaacgc ggacgccgga cgctcctggt cgatctcgat ccgcaggctg cactcagcgc
     1201 cgggttcggc atcgacgtgt actccctgga gaaatccatc tacgacgcct tgctgcacga
     1261 tcacgaaacg cctcttgcca gcgttcgcta caaccttcgc cccaatctcg acatcatccc
     1321 cgcgaacatc gacctggccg cggcagagat cgagctggtc gtcatgatgg ggcgagagtc
     1381 cgtcctgcga gaggcgctcg aaccgatcgc gaatgagtac gaatacatcc tcatagacac
     1441 gccacccagc ttgggcctcc tcaccgtcaa cgcgctggtc gtggcggatg agttgatcgt
     1501 gcccctgcag tgcgaatacc tggccatgcg cggcgtgcgt gtgttgttgg acacgatctg
     1561 gaaagtgaag cgcaagctga atcccaggct cgacctgctc ggtattctgc cgactatgta
     1621 ccgcagtcga tcgacgcaca gcaaggaggt tctggaagaa gtgcgcaata tcttcggcga
     1681 taaggtgttt gacatcgtta tcggcaacag cgtgcgtttt gccgaggcgc cggtggcgca
     1741 gcaaaccatt cttgagtacg agccacggca cgcgggagcg aaagcgtatc gttacttggc
     1801 ggaggtggtc gatgggaaga aagaaaccat cactgaaggg caggggggtt gacattctgc
     1861 tcggcgggtc ggccgacgag cagccgacag agcctgctgc gcagcctgtc gctgtgacgg
     1921 ccgagccggg gccgacgacc ctgcccgggt ctgtcgaggc cgcacccggg ccgggcgtag
     1981 aagccggccc gggtgcgccg atgcccaatc cattcgccga gccggcgccg gcgctcccgg
     2041 tctatgccgc caagccgacc acggaagccg gggtcgccga tttcaacccg tttgccgaac
     2101 cggcgtccgt gtcctcatcg gctgcggctc gacccattgc ggaagcggca gccacaatgc
     2161 ccaatccgtt cgccgaacca ctgccagcgg cggcggcggc gccttccgct gccgaagcgg
     2221 catcaatcgc gctgccgagt gatccaacgc tgttcaagcc agcccaatat ggaggaatcg
     2281 caatgagcat cgaggtcatg tccggctcgg gtgagtatca accgcccacg ggcgtcacgc
     2341 aaacgggtat gaaagcagtg acgctcacca gcaccggaga gcaggtgtcc gctgaagaag
     2401 tgctacagcg aatcggatcc gagcgcatcc aagggttgtc catgcgcgtc gacgatttgt
     2461 acgcccaggt cgccggcggc gcgatggcga acgcggagca ggccggggaa gccctgtcca
     2521 acctgcgcac cgcgcgcgac aaggagctgg aagacccgcg ccagtttgat gaagcggagt
     2581 acctggttaa tctggtgcag tatcagatca accattcggc ttcgatacag caggtccgga
     2641 actggtcgta tacctgggga gtggtcgtct tcatctacgg tgtgttctgg ctgctcgttt
     2701 tcggggctgg cattgctgtg tcggcgctcg ggctggtcga tcagtggctg accgacctgg
     2761 cacttgacga gcaaaccatc accaccgcgt ccgctttgtt cctcagcgtc acgtccggcg
     2821 gcctgggcgc ggtgatgggt ctgctgtaca gtctgaacaa gcacgccgcc atccagcagg
     2881 acttcgatcg acagtttctg ctgtggtact acgtccagcc cttcctgggt atgctgatgg
     2941 gcgtgatcgt caacctgttc ctattcgccg gggtattcct cgtggatgcg acaggcccgg
     3001 cgatccaagc gattggcgcg ctcctggcca tcgcgacggc cttccggcag aactacgtgt
     3061 atgcctggct tgagtcgctg ctcaggggat tcgagcgcag ggcgtcagcc gctgaagagc
     3121 gtagagaggc agaagcgccg aagcccgaag tgcagcccac gacacctacc gcgccagcgg
     3181 gcgaagcgca ggggtctcct gccggagtgg ggtgacggcg ctctcgattt ttccataacg
     3241 cgagccgagg tgattcacct cggctcgcgc gtttgaccgg gccggcggat tgcccggttc
     3301 agccattctt cacgcgctcg attttcgcgc cgagcgcgcg cagcttttcc tcgatgcgct
     3361 cgtaacctcg atcgatctgg ccgatgttgc gaatgacgct cgtgccctga gcgcacagcg
     3421 cggcgaggac cagggccatg cctgcgcgta tgtcggggct ggccacgcct cctggttccg
     3481 ggtgcagcca gctcggcccc tgtacgatgc agcggtgcgg atcgcacagg atgatgcgcg
     3541 cgcccatggc gatcaggcga tcgacgaaat acagccggct ctcgaacatc ttctcgtgga
     3601 acaggacggt ccccgccgcc tgtgaggcga ccacgatagc cgtgctcatc aggtcggctg
     3661 gaaaggcggg ccacggcccg tcgtcgatct tggggatggc gtcgccgatg tcgggcacca
     3721 cggtgagcga ctgcccgcgc gcgacgaaga tgtccggccc gcgcgcctcg aagcgcacgc
     3781 ccaggcggcc gagcatcagg cggatcatgc ccatgtgctc cggggccgcg tccctgacca
     3841 gcagctcgcc gccggtcatc gccgccagcc cgatgtagga tcccacttcg aggtagtcgg
     3901 gtccgatcgt cgcctcggtc ccgtgcagcg atcggacgcc gtgaatggta agcgtgttgc
     3961 tgccgatgcc ctcgatgcgc gcgcccatgc gactgagaag gtggcacagc tcctggacgt
     4021 gaggctcaga ggcggcattg cgcaggatag tcgcgccgtc ggccaggcaa gcgaccatga
     4081 tggtgttctc cgtgccggtt acactcgctt cgtcgagcaa gatgtccgcg ccggtgagct
     4141 tgtcggcccg tagtcgaaag cgtgcgttgg cctcgacttg cgcaccgagg gccacgaagg
     4201 ccaggaaatg cgtgtcgaga cgccgccggc cgatcacgtc cccgccgggc ggcggcagct
     4261 caacgtggcg gagccgcgcc agcataggcc cggccagcag aatcgacgcg cggatgtctc
     4321 ggcacaggtc ggcgttcaga tccgttttgc gcaggttggc ggcgcggatg caaaccgtgt
     4381 gcccgtcgtc gcgctctgaa acctcggcgc cgaggtcgca aaccagcttg agcatcgcgc
     4441 cgacatcgcg aatgcgtggg acgttgtaga gtgtgacagg ctcttcagtc aggagcgcag
     4501 cggcgatcaa agggagcgct gcattcttgt tgccgctcgg gacgacggcg ccgccgatcg
     4561 ggtagccgcc ttcgatgaca aacgtttcca tgtgtgagga atttccccta taggttggct
     4621 gcacagcctc gaattccctg taccaggcga ttgtcaacgt tatggtcaat gcgatagtac
     4681 catacggcca caaggcatgc aacccgggcg tcctatggct cgcctacggg tgggcgacga
     4741 ggctatcttt cgcgagttgc tccggtcgag tctgtctcgc acatctgttt tactaatccc
     4801 gcttaccgtg gtaaaattac catctgtggc gcagatctcg ggtaagcagc cgctcggtat
     4861 tttgacggcc ctgtcgaccg ggtttgacgc cgtcacgcgc caactctggc tcttggcgct
     4921 gccggcggcg ctcgacgtat tcttgtggct ggggccgcgc ctgaaaacgc cgggtgtgtc
     4981 gcgcctgttc atgtttgaaa tcccggcggg tctggacgcg aatacgcgcc tgtttgcaca
     5041 ggatttccag gattcgctcc ggcacgtcct tgagaatgcc aactggtttg tgtggctgct
     5101 tcggcctgcc ctgcttggcg ttccgggact ttccgacgcc ctgggggagc ctcctcccgg
     5161 cgtcacgcca accgtatggc cgcttgcgga tataggagct ttggtggcgg cagtgatcgt
     5221 tctggtcgtg cttggcgtcg gcttgagcgg gctttactgg agcctcgtcg ccaggcaggc
     5281 acgcgacggg cgtatcgact ggtgggcagc ctggggacgc atggctgtcg tgtggccgcg
     5341 tatgctggct ctggcgctcc tgctcatcgg aatggtaacc gccatctgga cgccagcgct
     5401 gattctcagc gcgttgctcg gatccgcgct cgggctgtct ggcgcgctgc tcgtcatggt
     5461 gtcatttgcg ctgctggtgt ggctcctgtt ttacacggca ttcagcgtcc acggcattgt
     5521 gctgtataac cagcgcgtgc tggatgccgt gcgggcgagc gtcgatctgt gccgcgctca
     5581 tttctggaaa acgctcggcc tgctcgccgc gctggtcgcc atcgaatggg gcatgggcct
     5641 gatctggaat caggtgcctt ccggctcgtg gctgtgggcg gtgtcgatcg ccggcaacgc
     5701 gttcgtcgtc gccgggctgt cgatggcgtc tatgatgtac tacatggaac acgtccccgt
     5761 gccgcgctcg acgcttgtgc cgcagtgacc cggagaaacc tggtttctct aagagaccaa
     5821 gtttctaact gaggagacta tgtcaaacga gaagccgtcc acttacgatg ccagtcaaat
     5881 tcaggtgctc gagggcctgg aagccgtgcg ccgccggccc ggcatgtata tcggctcgac
     5941 ggacattcgc ggcctgcacc atctggtgta cgaggtcgtg gataacgcga tcgacgaggc
     6001 gctggccggc cagtgcgatc agatctacgt cacgattcac aaggacctga gcgtgtcggt
     6061 gcaggacaac gggcgcggca tccccgtcgg cccgcatccg acgcaaaagg acgccagcgg
     6121 caagccgatg gacgcgctgg aagtggtgat gacgatcctg cacgccggcg gcaagttcgg
     6181 cggtgaaggc tacaaggtcg cctcgggcct gcacggcgtc ggcgtgagcg ccgtcaacgc
     6241 cctgagcggg tggatggaga caaccgtgcg gcgcgacggc aagatttatc gccaacgtta
     6301 cgagcggggt aggccgaccg gcaaggtcga gcaggccggg cgcatcctga aggacgatcc
     6361 ctacccgcac ggcacgctgc agcgcttcac gcccgacccc cagcttttca aggaaggcat
     6421 cgactacaag ttcgagacgc tggcgcagcg cttccgcgag atggctttcc tgacgaaagg
     6481 actgacgatc acattctgcg acgagcgcga cggtcacgag atgtcgttct acttcgaggg
     6541 cggtgtccgc tcgttcgtgc gctacctgaa caagaaccgc gccgtgctgc acgatccggt
     6601 gcacctggag aagcaggtca acggcaccgc cgtcgaggtg gcgattcagt acacggacgg
     6661 ctacaacgag tcggtcttcg ccttcgccaa caacatcaat accgtcgacg gcggcacgca
     6721 tctcaccggc tttcgatcgg cgctgacgcg cacgatcaac gattacgcgc gcaaggccgg
     6781 gctgctgaaa gactccgatc cgaatttcag cggcgaagac gtgcgcgagg gcctgacggc
     6841 gattgtcagc gtgaagctac aggacccgca gttcgagtcg cagacgaagg ggaagctggg
     6901 caacgccgaa gtcaaaggtc acgtcgagtc ggtggtgtcc gaggcgttcg gcgagttcct
     6961 cgacgaaagc ccgcgcgagg cgaagcagat cgtgcagaag tgcctcacct cgtcgcgcgc
     7021 gcgcgaggcc gcgcggcagg cgcgcgatct ggtgattcgc aagtcggcgc tcgagtcgct
     7081 gacgctgccg ggcaagctgg ccgactgctc ggagcgtgat ccgtcgaaat gcgagattta
     7141 cattgtggaa ggcgattcgg caggtggctc ggccaaacag ggtcgcgacc ggcacttcca
     7201 ggcgatcctg ccgctgcgcg gtaagattct caacaccgag cgtgcgcgga tggacaagat
     7261 cctcgccaac aacgaagtca aggcgctgat cagcgcgctc ggctgcggca tcggcgatca
     7321 gttcgatttg agcaacctgc gctaccaccg cgtgattctc atgagcgacg ccgacgtgga
     7381 cggcagccac atccgcacgc tgctgctgac gttcttcttc cgctacatgg agcagctcat
     7441 cgagaacggc cacctgtaca tcgcgcagcc gccgctgtac cgcatcgagg tgaagaaggg
     7501 cgcgaaggac gttgtgtacg cctattccga cccggagcgc gagggcatct acgtccagct
     7561 tcgcaagaag ggactcgacc ccgccgacga caagcaggtg gtgacgcagc gttacaaggg
     7621 cctgggcgag atgagcgccg agcagttgtg ggagacgacg atgaacccgg agaatcgcac
     7681 gctgctgcag gtgacgatcg aggacgcggc cgccgccgac aagaccttcg acatgctgat
     7741 gggcgacgag gtcccgccgc gcaagcgctt catccaaacg cacgcgaagg aagtgcggaa
     7801 cctggatatt tgaggcgtat tagacccgac ggggctgcta gtaccgaacg gaaaaccatt
     7861 gaccactgag cgcctgagcg aagctttagt ctttgctgca cacttgcatg ccaggcaaac
     7921 tcgcaaaggc acacacattc cctacattgc ccacctgatg gccgtcgccg gcctcgtact
     7981 ggagaacggc ggggacgagg acgaagccat cgccgcgcta ttgcacgacg ccgtcgagga
     8041 ccagggtggt gccgcgacgc gcgaaaacat ccgccgccgc ttcgggggca gggtggtgga
     8101 gatcgtcgat ggctgcacgg attcggacag agttccaaag ccgccgtggc gcgcacgcaa
     8161 agaagccttt ctcgcgcgtc tcccccgggc ttcttcgtcg gtgcgactgg tgtccgccgc
     8221 cgacaagctg cacaacgcgc ggacgattct ggccgattac cgcgcgatgg gcgatgtggt
     8281 gtggaagcgc ttcacaggcg ggaagagcgg aacgctgtgg tattaccgtg cgctcgtcga
     8341 cgctttctgc gcggcgggca cgtcgtcgtt ggtcgaagaa ctggcgcgcg tcgtcgcgga
     8401 gcttgatcaa gtcgagcggc aaacgggctg attcgctcgc ccactgtcat gggtctcctc
     8461 gtgtgtcgcg gcggtttttc gtaggggcga cccaggcggt cgccctgggc aggcgcgaga
     8521 cctgccccta cagcgggtgc cccatgttac gcgcgatgcg cgcgttgcgc aacttgcgag
     8581 cgtggtataa tgagaaactt ggttccgggg ctacacgaga ggagattgca ggatgaaggt
     8641 gcgcgaccga gaaatccgcc gacgccgggc gcgccggcgc aagacctggc gtctcaaggc
     8701 gcgcatggcg gcgacgacgg acagcagggc caaggccagg ctcgccgaga agatccgccg
     8761 cgtcaatccc tacgtgcaag atgcaaagta ggccggcccg tgcgcaacag gccgctccgc
     8821 gcgagcggcc ttttttgcgt gcgacgcact tcgcaagcgc gtcgcacgtc ctcccaactt
     8881 gcacgcgggt gcatgcttga ggcgggtata atgggataga ttcatcgagc gtgggtggcg
     8941 gtgagagcgt gaattcgtca caggcgatcg agacggcgaa cctccgcaag gtctacaacg
     9001 gcaaggtggc cgtggccgac ctgagcctga gcgtgccgcg cggcgaagtg ttcggtttcc
     9061 tcggaccaaa cggcgccggc aagacgacgg cgatcaagat gctgctggga ctggctgcgc
     9121 cgaccagcgg tacgggccgc cttctcggca ggccgatcgg cgatccggct gcccgcgccc
     9181 gcgccggttt cctgcccgag catttccgct tccacgattg gctgacgggc gccgagttcc
     9241 tccatctgca cggacagttg tatcgcatga gagaggtcga tcgacggcag cgcgtgcccg
     9301 agctgctggc acgcgtcggg cttgccgaac atggcgacaa acgcctgcgc ggctactcga
     9361 aagggatgct gcagcgcatc ggcctggcgc aggcgctcct gaaccggccg gagatcgtgt
     9421 tcctcgacga gccgacgtcg ggactcgatc cggttggccg catcctggtg cgtgacatca
     9481 tccgcgatct gcgcgagcaa gggacgaccg tctttctcaa ctcgcacctg ctgtccgagg
     9541 tcgaaatcac gtgcgaccgc gtgatcttca tcaagcacgg cgccgtcgtc cgatcggtgt
     9601 cgctcggcga aatggggcag ggggcgctgc cggtcgaaat ccgcatcggg gcgctgccgg
     9661 ccggcgcgca agcggatctg ctgtcggggc tgtcgcgctg ggggcgcgac gcgcgcgtcg
     9721 aagacgggcg cttgcacgtg atcgtcgacg acgaggcctc tcttcctgag atcgcgcgct
     9781 ggctggttgg ccagggcatc gacctctacg gactgatccc gcagcgcatg tcactcgagg
     9841 acatgttcct gcagatcgtc ggcacagacg gggggctgtg acgtggccgc gcgcatcatc
     9901 accgggttga cgatccgcga ggctcgccgc cgcaaggtgc tgtgggggct gctcatcctg
     9961 agcgcactgt tcttggggtt gtacacgctg ggattgaact tcgtctacac cgagatcatg
    10021 agtcacggcg ggccgcccag gggggcttcg cgcctcttct cgttggacga cgtgtggaac
    10081 ttcttcctga cggcggcgct gtacggcgcg aatttcctgc tcgtgatgat ggccgtgctg
    10141 atctcggtcg atacgctggc gggcgagatt tcgagcggca cgatccagtc tatcgcggtc
    10201 aagccgctcc ggcgtcacga aatcctgttg ggtaagtggc tcggcttcgt cgtcatgctc
    10261 gccggttata cgctgctcct ggccggcggg gtcatcgcga tcactgcgct gatcaccgga
    10321 tatgcactgc cagcctggct gccgacgctg gtgttgatgt atctcgaagc gctggttctg
    10381 ctgagcgtgt cgatcgtcgg cggcacgcgc ctgtcgacgc tggcgacggg cgtgctgggt
    10441 ttcggcctgt tcggcgtcgc gttcatcggc ggctggatcg agcagatcgg cagtttcatc
    10501 ctcgtgcaga gcgaggcggc cgtctcgatc ggccgcctgg cttcgttcgc catgccgagc
    10561 gaagcgcttt ggcggtacac cctatcggag gtcacgacga gccggaaccc gttcagcttt
    10621 atcttcggcg gcacgtcggc tcccgatgca ggcgtcgtcg tgtatgccat cgcatatgcc
    10681 gtcgcgctcc tggcgctggc cgtgctatcg tttcggcggc gcgatctgta gcgctggttg
    10741 ctttctcctg aggaatagac ctggcgggtc cccccaagac ctgccaggtt ttttttgact
    10801 tttcgcgcgt cgcgaataca cttggcagca tcccacgacc atctcggcat tgtctaccag
    10861 ggggaaacca tgaacgacag gcgagagata ttcggctggt cgatgtacga ctgggccaac
    10921 tcggccttct cgacgacggt
//
LOCUS       DBNHXB010000091         7235 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1694027, whole genome shotgun sequence.
ACCESSION   DBNHXB010000091 DBNHXB010000000
VERSION     DBNHXB010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7235)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1694027"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="AC162G_03505"
     CDS             <1..1060
                     /locus_tag="AC162G_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005085 - guanyl-nucleotide exchange
                     factor activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HIR5074913.1"
                     /translation="LTPVDVSGLTSGVAALAAGWIHTCALTAGGGAKCWGDNDYGHLG
                     DGTYTDRLTPVDVSGLASGMAAVAAGGGHACALTAGGGAKCWGANGAGQLGDWTTTTR
                     QTPVDVIFPVSTNYLPTIFQNYCTDFYDDFSNPGSGWFVGENNELRVEYLNGEYRVLV
                     KPAGYLFVFRAPTCDQQDYVVEADARWAGSPGASYGLIFGIVGNFEWFYSFEVNTDSQ
                     EYALFRYSPGGWDVIAPFTYSPAIQAGTATNHLKATRDGYNITLEANGSFLGTWFDNI
                     IAGEVGTGLIVSSYDDRPNADARFDNFKVTYGPFMRVTTADRDTTIGPAQPPEDGAFG
                     AAAPKDLYWQLQREEIWP"
     gene            1221..1496
                     /locus_tag="AC162G_03510"
     CDS             1221..1496
                     /locus_tag="AC162G_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074914.1"
                     /translation="MNRGRNLGAALPWYILSIISLCLFPVSGTFQTSGSGFFGSSGSA
                     GWSWDSGSGSSDSSGGWEWGGGDSGGWDSGGGDSGSWDSGGGDSGGW"
     gene            1499..2419
                     /locus_tag="AC162G_03515"
     CDS             1499..2419
                     /locus_tag="AC162G_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR5074915.1"
                     /translation="MSNNMRNWLICGVLLNILVWSLLFLPPFFYLLAAFFAFAIGNAQ
                     LQTRVGTPALARPGGLPVRTLSQAPVRSAATARVELPERKMPDNIPANLKCPSCGANI
                     KPTDKKCRFCGSALQPLLDLPEPVKLGALEIGRRVKVVHPQMGGQIYRVLGRLLYTEL
                     WQAASGPAVPWTPTGNYYAGFALDPAAYLLNWQDRFYILEERRSLTDMDVNRDFVPYA
                     RQFAQSNQTARVDFRYAGERWRIVDIGRFAIEFEDGEGLHLHKGAIGRFIHASNGDQA
                     LVVDDFQSGGSGGQDTLWKGFQIKEADITF"
     gene            2435..2767
                     /locus_tag="AC162G_03520"
     CDS             2435..2767
                     /locus_tag="AC162G_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074916.1"
                     /translation="MRRIFGRFTLIITLSLVLVVGQVVSYFASPASYQGFVGVLLRIL
                     SMIAFWGPIIALISSIFVWVVMNLLGFESLEAIRQESVEQNNPVPAILFVGTLIASVL
                     FLMLVIKP"
     gene            complement(2851..3429)
                     /locus_tag="AC162G_03525"
     CDS             complement(2851..3429)
                     /locus_tag="AC162G_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074917.1"
                     /translation="MKYTVFVLVALLTVACSSGPSQADLEATVSAVVADAVEATVEAT
                     YPGSIARPYPIGQSTRIRLGDGQEFLLTVAEIIRGGEAFDIVIDANESNDEPPEGMEY
                     LLARIKVEYIGPEAGALELDQHDWSVMTNGQLHDFLALPAVACCLAPEFDISLHSGGT
                     GEGWLPVVVSVDDTKPLLVWGGPGGIYFAAQE"
     gene            4098..>7235
                     /locus_tag="AC162G_03530"
     CDS             4098..>7235
                     /locus_tag="AC162G_03530"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR5074918.1"
                     /translation="MRRSTTAACLAFSASLLAASASTSQGAPPHLAPQALPPGYTLAA
                     VASIQWPAEIQCVGDGRCFVASVHDPTDEERSAIWVIHPDGSQQRIYRGPHYEPQLLG
                     EKGLTGMALHPRFEADDRIFFYWITPSVNRTDPIWAVISSIRSDGSDYHEVWRSTQKQ
                     FTDSHVAGGLVTYAENGHDYLFVAIGEFGAGKSQDLTVEQGKIHRFEIVGDTLQAAPD
                     NPFYDTPGAVRSIWASGFRNPFRLTRDPATGEVYITENGFRCSDRVYRLVKGGNYGWP
                     TWDWCQDNPGYVQPIYEFRPSIGVTDIEVYHGPIPGWEGKVFICGFVPGPLVMLTLAA
                     DGRLIDRQDVTDSVHTCGLALGTRADGVLLFSKQDQAGTIYAVTPLNPGPRIAAQLTS
                     DAAQPAADRRIHYALDISNLSFANSVTATIRLPDALGYLPGSTFGGAIYDGSLRQITW
                     TPSIGAGRALRAGFDALASGPPGTLISVTAQIVDRYGQATAPSASIVITAGSALDVSV
                     MPGSPAIQSGQSVTYHLRVHNQAISDTTFSLTMTLPGEAQFVRHIFSPTTTYDDGLRA
                     ILWQRALKADRQAQTAVVVSATQSLERMIPFTTTTSPGSGFTSTRVTKLLLDPYQSFL
                     PLVTKPFGSSPPAVAFSLTPDDHPFGRFGTPPGSLDNLIGPSNCQVCHDAAGSSIYAA
                     WFTSAHGTSGFDPVFKAALHNASQQVQDVERWCTQCHQPSNWLGGHPLDAQDTSGITC
                     AVCHRAVDSDSVASEDQPAIQRAMAMGLIPPRWKEIGGNGALIVDHNDVRRGTNFLND
                     SAPHQTAMSDFQGSSALCGACHSVYAPHLARDLTGEFIPGTPDIASGENPLFLQSTYP
                     EWLHSQYAAAGTQCQDCHMPRQPGYVADPDKGGLPRDVPAHHFAGGNLFILDILEQTG
                     QLTGSAAQRAAVASMLGQAATITGSVSGTTLTVAVTCLAGHKCPTGYEEGRAWVLQVE
                     QLDANGQRIACSGCWSELAQTIAGYDAEPGDAGYDPELTVFGVRFGITGTHALGVGRP
                     TGETFNLALNNVVLSD"
BASE COUNT         1367 a         2325 c         2169 g         1374 t
ORIGIN      
        1 cctcacaccg gttgacgtga gcggcctgac aagcggggtg gcagccctgg cagcaggctg
       61 gattcacacc tgcgcgctga ctgcgggcgg cggagccaag tgctgggggg ataacgacta
      121 tggccacctg ggggatggga cctacaccga tcgcctcacg ccggtcgacg taagcggcct
      181 ggcgagcggt atggcggcag tggcggcagg aggggggcac gcctgcgccc tgacggcggg
      241 tggcggggcc aagtgctggg gggctaacgg ggctggccaa ctgggcgact ggacgaccac
      301 cactcgccag acgccggtgg atgtcatttt ccctgtctcc actaattacc tgcccactat
      361 tttccagaac tactgcacag atttctatga cgacttcagc aacccgggca gcggttggtt
      421 tgtcggcgag aacaacgagc tgcgagttga gtacctgaac ggcgagtatc gcgtgctggt
      481 caagccggcc ggttacttat ttgtgtttcg cgcgccaacc tgcgaccagc aggactacgt
      541 cgtggaagcg gacgcccgct gggcgggcag tcctggcgca agctatggct tgatcttcgg
      601 gatcgtgggc aacttcgagt ggttctattc gttcgaagtg aacacggact ctcaagagta
      661 cgcgcttttc cgctatagcc ctggaggctg ggacgtgatt gcccccttta cgtactctcc
      721 agccattcag gctggcacag ccacaaacca cctgaaggct acccgcgatg ggtacaatat
      781 caccctggaa gccaacggtt ccttcctggg aacctggttt gacaacataa tcgcaggtga
      841 ggtgggcacc gggcttatcg tcagctcata tgatgatcgg ccaaacgcag atgcccgctt
      901 cgacaacttc aaagtaacct atgggccatt catgcgtgtt acgactgctg accgagacac
      961 aacaattggc ccggctcaac cgcccgagga tggtgcattt ggagcagcgg cgccaaagga
     1021 cttgtactgg cagctacaga gggaggaaat ctggccttag atccgaacca cccaccagga
     1081 ggtcacaaga gataagtctt gccgcgtcga aaggtttgcc ctgcgagggg atcaagaggg
     1141 agctgagtcg ctcaaccggg agtgtacatg agccaacaca ctcaaccagt cagcgttgtc
     1201 cttaagatgg aggaatcaag atgaatcgtg gccgaaatct cggcgccgcg ctgccctggt
     1261 acattctgag catcatctcg ttgtgcctgt tcccggtaag cggcaccttc cagacgagcg
     1321 gcagcggatt cttcggctcc tccggctcgg ccggctggtc gtgggattcg ggcagcggtt
     1381 cgtccgacag cagcggcggt tgggagtggg gcggcggtga cagcggcggt tgggactcgg
     1441 gcggcggcga cagcgggagc tgggactccg gcggtggcga tagcgggggc tggtgactat
     1501 gtccaacaat atgagaaact ggctcatctg cggcgtcctg ctgaacatcc tggtctggtc
     1561 gctgctcttc ctgcctccct tcttctacct cctggcagcc ttttttgcct tcgcgatcgg
     1621 caatgcccag ttgcagacgc gtgttgggac acccgccctg gcgcgtccgg gtggactgcc
     1681 cgtcagaacc ctgtctcaag cgccggtccg gtctgcggcg acggcccgtg tggagcttcc
     1741 ggagagaaaa atgcccgata atatccccgc caacctgaag tgtccctcgt gcggcgcgaa
     1801 catcaaacca accgacaaga aatgccgctt ctgcggttct gccctccagc ccctgctgga
     1861 cctgcctgaa ccggtcaagc tgggcgcgct ggagatcggc aggcgcgtga aggtggtcca
     1921 tccccagatg ggcggacaaa tctaccgggt gctcggccgc ttactctaca cggaactctg
     1981 gcaggctgcc agcggaccgg cggtcccgtg gacgccgacc ggcaactact acgccggctt
     2041 cgccctggac ccggcagcct acctgttgaa ctggcaggac cgcttctaca tcctggagga
     2101 gcgccgctct ctgaccgaca tggacgtcaa ccgcgacttc gtaccctacg cccggcagtt
     2161 cgcccagtcg aaccagacgg cgcgggttga tttccgatac gcgggcgagc gctggcggat
     2221 tgtggacatc ggccgctttg ccatcgagtt cgaggacggc gagggcttgc acctgcacaa
     2281 gggcgccatc gggcgcttca tccacgccag caatggggac caggccctgg tggtagacga
     2341 cttccagagc ggtggcagcg gcggacagga cacgctctgg aaaggtttcc agatcaaaga
     2401 ggcagacatt acgttctaat tcataggaga acctatgaga agaatctttg gacgtttcac
     2461 gctcatcatc accctgagtc tggtgctcgt ggtcgggcag gtcgtttcct acttcgcctc
     2521 accggcctct tatcagggct tcgtcggcgt cctgctgcgc atcttgagca tgatcgcttt
     2581 ctggggaccg atcattgccc tgatctccag catctttgtc tgggtagtga tgaacctgct
     2641 cgggttcgag tcgctagagg ccatccgcca ggaatcggta gagcagaaca atcccgtccc
     2701 tgccatcctc ttcgtcggga cgctgatcgc cagcgtgctg ttcctgatgc tggtcatcaa
     2761 gccgtaatcg tcttccttcc acgctgagga agcgcctggc gcgaccgaag ccctcgttcg
     2821 attgcagagg ccaaggatgc aaacgccgcc ctactcctgc gctgcaaagt agatcccacc
     2881 tgggccaccc cacacgagca acggcttggt atcgtcgacc gagacgacga ccggtaacca
     2941 accttcaccc gtgccaccag agtgcagaga gatgtcgaac tctggcgcaa ggcagcatgc
     3001 gaccgcgggc agcgccaaga aatcgtggag ctgcccattg gtcataaccg accagtcgtg
     3061 ttggtccaac tcgagtgcgc cggcttccgg cccaatgtac tccactttta tacgggcgag
     3121 cagatactcc atgccctcgg gtggctcgtc gttggattcg ttcgcatcga tgacgatgtc
     3181 aaatgcttcg cctccgcgga tgatctctgc aacggtcaat aagaattcct ggccgtcgcc
     3241 cagtctgatg cgtgtcgact ggccaattgg atatggtctg gcaatcgagc caggataggt
     3301 tgcctcgaca gtcgcttcaa cagcatcggc tacgaccgca gaaacggtgg cctcaagatc
     3361 ggcttggctg ggaccactac tgcaagcaac ggtcaaaagc gcgactagaa caaatacggt
     3421 gtacttcatc agatgctcct tatgccttga tagtatctca actaataaac gtatcggcga
     3481 acaaaattca gctattgttc gcatttacac aatcaagatc cgccgtgaag cgcagtccaa
     3541 agctgccaca cttgccgtct tgccgtttca gcagtctgac cacccgccgc ggtttcgtcg
     3601 gttcgtgccc caggcggcct ccccagtaca ccgagtcgcc atcatgcgga cgcttgtcgc
     3661 tccgtgcttt cgcgtggcgg cggatggctg tatcctggtg aaccgaaaaa ttgtgcttga
     3721 ctccggtcga tttcgatata cactgtcata ctgaatgacc gtcagagaca cgatgagaac
     3781 tgtcaaccaa ctcgccctac tgctaaccgc acggtaacgt gccatgttgg gtggtggcgc
     3841 ccagcccaga cttggtcact tgattctgtg attggcatca taagtcaacc ggtcgtcaca
     3901 gggcgtctga gagcacttga aggcctattc gcctttatgt tcatggctga ccgagtatcc
     3961 atcagtctgc aattcggaga tggccagttg ctacatcttg gcactgccgg caagcctgct
     4021 tctgtcgagc tactccatct gctgcctgct gccattggca agccgttaga acagagagaa
     4081 cagtaggcga gattgttatg cgtcgatcca ccacagcggc ctgcctggcg ttttctgcca
     4141 gcctgctggc tgcctccgcg tcgacctcac agggcgctcc tccccatctc gcgccgcaag
     4201 cgctgccgcc gggttacacc ctcgcggcag tcgccagcat ccagtggccg gctgaaatcc
     4261 agtgcgtcgg tgatggccgc tgcttcgtcg cgtcggtgca cgatcccacc gacgaagaac
     4321 gcagcgcgat ctgggtcatc cacccggatg gcagccagca gcgcatctat cgcggcccgc
     4381 attacgagcc gcagttgctg ggcgagaagg gcctcaccgg catggcgctg catccgcgct
     4441 tcgaggcgga cgatcgcatt ttcttttact ggatcacgcc gagcgtcaat cgaaccgatc
     4501 ccatctgggc cgtgatttcc agcattcgct cggacggcag cgactaccac gaagtgtggc
     4561 gctcgaccca gaagcaattc accgattcgc acgtggccgg cgggctggtc acttacgccg
     4621 agaacgggca cgattacctc ttcgtggcga taggcgaatt cggcgcgggc aagtcgcagg
     4681 acttgacggt tgagcaaggc aagatccatc gcttcgagat cgtcggcgac acgctccagg
     4741 ctgcccctga caatcccttt tacgatacgc ccggcgcagt tcgctcaatc tgggcgtccg
     4801 gcttccgaaa ccccttccgc ctcacgcgcg atccggcgac cggcgaggtg tacattacgg
     4861 agaacggctt tcgctgcagc gaccgtgtgt accgtctggt caagggcggc aactacggct
     4921 ggccgacctg ggactggtgc caggacaacc ccggctacgt tcagcccata tacgagttcc
     4981 ggccgtccat cggcgtcacg gacatcgaag tctatcacgg cccgatcccc ggctgggagg
     5041 gcaaagtctt tatctgcggc tttgtcccag gcccactggt catgctcacc ctcgccgccg
     5101 acggcaggct gatcgaccgg caggatgtca ccgacagcgt gcacacgtgc ggactggcgc
     5161 tcggcacgcg ggccgacggc gtgctgctgt tctccaagca ggatcaggcc ggcacgatct
     5221 acgccgtcac gccgctcaat cccggcccgc gcatcgccgc gcaattgacg agcgatgcgg
     5281 ctcaaccggc tgcggatcgg cgcatccatt acgcgctgga catttcaaat ctctcgttcg
     5341 ccaactccgt cacggcgacg atccggctgc ccgatgcgct cggctacctg cccggctcga
     5401 ctttcggtgg cgcgatttac gacggcagcc tgcggcagat cacgtggacg ccgtcgatcg
     5461 gcgccggccg agcgctgcgc gccggtttcg atgcgctggc ctccggcccg cccggcacgc
     5521 tcatctccgt caccgcccag atcgtcgatc gctacggcca ggcgaccgcg ccgagcgcgt
     5581 cgatcgtcat caccgccggg tcggcgctgg atgtgtcggt gatgcccggt tcacccgcga
     5641 tccagtccgg ccagagcgtc acctatcacc tgcgcgtgca caaccaggct atcagcgaca
     5701 cgactttcag cctgaccatg acgctgccgg gtgaagcgca gttcgtgcgg cacatcttct
     5761 cgccgaccac gacctacgac gacggcctgc gcgccatcct ctggcagcgc gcgctcaaag
     5821 ccgatcggca ggcgcagacg gccgtcgtcg tcagcgccac acagtcgctg gagcgaatga
     5881 ttccgttcac caccacgacc tcgcccggca gcggcttcac gtccacgcgg gtgacgaagc
     5941 tgctgctcga cccataccaa tccttcctcc cgctcgtcac gaagccattc ggatcatccc
     6001 caccggcagt cgctttttcg ctcacaccgg acgatcatcc attcgggcgc ttcggcacgc
     6061 cgcccggcag cctggacaat ctgatcggcc caagcaactg ccaggtctgc cacgacgcag
     6121 ccggaagctc gatctacgcc gcctggttca cctccgcgca cggcaccagc ggattcgatc
     6181 cggtgttcaa ggccgcgctg cacaacgcct cccagcaagt ccaggacgtg gagcgctggt
     6241 gcacgcagtg ccaccaaccg tcgaactggc tgggcggaca tccgctcgac gcgcaagaca
     6301 cgtcggggat tacgtgtgcg gtctgccacc gcgccgtgga ttcggacagt gtcgccagcg
     6361 aggatcagcc ggcgatacag cgggccatgg cgatggggct gatcccgccg cgctggaaag
     6421 agatcggcgg aaacggtgcg ctgatcgtcg atcacaacga cgtccggcgc gggaccaact
     6481 tcctcaacga ctccgcgccg caccagaccg cgatgagcga tttccagggc agctcggcgc
     6541 tgtgcggcgc gtgtcacagc gtctacgcgc cacacctggc gcgagacctc accggcgagt
     6601 ttatacctgg cacgcccgac atcgccagcg gtgagaaccc actgttcctt caatccactt
     6661 accccgaatg gctccattcg cagtatgcgg cagccggaac gcagtgccag gactgccata
     6721 tgcccaggca gccgggctac gtggccgatc cggacaaggg tgggctgcca cgcgacgtgc
     6781 ccgcgcatca ctttgcgggc ggcaacctgt tcatcctcga cattttggaa cagaccggcc
     6841 agttaaccgg atccgccgca cagcgcgccg ccgtcgcgtc aatgttgggc caggcggcaa
     6901 cgattaccgg cagcgtgtcg ggcaccacgc tcaccgtcgc cgtcacctgc ctggccggac
     6961 acaagtgccc aacaggctac gaagaagggc gcgcctgggt cttgcaagtc gagcagctcg
     7021 acgccaacgg tcagcgcatc gcctgttccg gctgttggag tgaactcgcg cagaccatcg
     7081 cgggatacga tgccgagccg ggcgacgccg gctatgatcc ggaattgacg gtattcgggg
     7141 ttcgattcgg tatcacgggc acgcacgcgc tgggtgtggg caggccaacg ggcgagacgt
     7201 tcaacctggc tctcaacaac gtcgtcctga gtgac
//
LOCUS       DBNHXB010000092        13809 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1022936, whole genome shotgun sequence.
ACCESSION   DBNHXB010000092 DBNHXB010000000
VERSION     DBNHXB010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 13809)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13809)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13809
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1022936"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="AC162G_03535"
     CDS             complement(<1..1099)
                     /locus_tag="AC162G_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N' family DNA-binding
                     protein"
                     /protein_id="HIR5074919.1"
                     /translation="MLSQASLFAEIVILKHIGRASEEGRAFHYEVPDDLRGRLAPGHL
                     VVVPLRERRMPGVVVALSESSPVERTRPIESLLDPSPVLSPLQLDLARWISQATLAPL
                     NECIDLFVPAGIAGRVDTLYAMAVDSVPERMNATQSEVFALLNRRGPLRGAQIARALH
                     RKPWQDAMRSLTRRGVVARRPVLLPPSVRPKTARFARLVVDPAGIEAKMLSERADVAA
                     RRASVLRLLADKSGPVLLSDIYAATDCSLSDLQILAEREFITLSEAEVWRDPLAGKEF
                     VPTDAPTLTDDQRAVWEQIEGVISSDDFSRRGATEVATTAFLLRGVTGSGKTEIYMRA
                     VDAMLQRGRQAIVLVPEIALTPQTVRRFAARF"
     gene            complement(1103..2449)
                     /locus_tag="AC162G_03540"
     CDS             complement(1103..2449)
                     /locus_tag="AC162G_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR5074920.1"
                     /translation="MTESVFGLIAIVVLVLANGFFVAAEFSLVSVRPTRVEELVRGGT
                     PGAAAVKHAIKDTDRFIAATQLGITLASLGLGWIGEPALAHLIEPLLAFLPGEIAGEA
                     AHIVAGTAIAFAIITFMHVIVGELMPKSIALMNPERTALFIAGPTLIAETIFRPFIWL
                     MNGTGNFLLRLIRVRPAAHGTLHSIEELKMIAEASEQGGLIEPDEREMIHAVFDLRDT
                     SARQVMVPRMEMVTVDATATLEEALDVAAHSSLTKFPVYENDPDHVVGVLHVKDVVRV
                     IHESIEQDAGRAALAKTAREIMREALFVPETVRVDDLIARMRRQKQHMAILLDEFGGT
                     AGLVTLEDLVEELFGDVQDEFTIEEPDLVPRADGSISVSGLMLIEDVNDALGVNLVDQ
                     NSDTIAGYVLSRLGRIPAAGDSLQADGVHFRVERMDGLRIDRIGIRVDERRKTKDE"
     gene            complement(2499..3275)
                     /locus_tag="AC162G_03545"
     CDS             complement(2499..3275)
                     /locus_tag="AC162G_03545"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR5074921.1"
                     /translation="MNQPDASQAVPGAPAPGQRVALRLPLSRPRWMWIFLAANVAVWL
                     LMTLAGGSEDPVVLIQFGANFTPFVAAGEYWRLLAANFIHIGIVHLLFNSYAIYVLGA
                     DVEALYGPPRFVVVYVLSGLAGSIASFALRADIGLSAGASTAVFGLLGTMIAFFVRHR
                     DRFGGWGRQRLGNYVAVVGVNLLFGLTVPRIDMLGHIGGFAGGLVLGWLLCPMYQVEY
                     APERPRLVDTNSMRRALPGVALFVAFLIIGVMLGVARWNV"
     gene            complement(3272..3892)
                     /locus_tag="AC162G_03550"
     CDS             complement(3272..3892)
                     /locus_tag="AC162G_03550"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR5074922.1"
                     /translation="MSETDLYRRPTHPLLIVVSGPSGAGKDTLLARLKEKNLPFHFVV
                     TANTRPRRPGEVEGRDYHFVSRDEFVAMIERDELLEYAVVYGDYKGIPKQQVREALAS
                     GTDVIMRLDVQGAMTIRRLVPEAVLIFLMAGDEGELADRLRTRRTETPDSLKTRMDTA
                     RQELQHLPEFDYVVVNRNGALDDAVDAVLGIVRAEHSRVHPRKVSL"
     gene            3988..4410
                     /locus_tag="AC162G_03555"
     CDS             3988..4410
                     /locus_tag="AC162G_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR5074923.1"
                     /translation="MPIYEYRCPNCKKRVSVFFRSLSSVDHEAARCPNCGSGGLARLM
                     SRVRLVRGSSKSSGDGGDDDFGDDMLDSIDENDPRSLGRFMRKMAEESGEDLPPEFNE
                     VVGRLEKGESPEDIEKSMPELGDMAGDEGDGDFGGDDE"
     gene            complement(4465..5100)
                     /gene="tmk"
                     /locus_tag="AC162G_03560"
     CDS             complement(4465..5100)
                     /gene="tmk"
                     /locus_tag="AC162G_03560"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019010276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR5074924.1"
                     /translation="MSLFVTFEGPDGSGKTTQVARLTERLRAAGHDVIATREPGGTRI
                     GDQVRAILHAPGNRSMRPVTELLLYCASRAQLVSEVIRPHLERGGIVLSDRYADSSLA
                     YQGYGRGIDPASLRAILAFATDGLRPDLTLLLDLDAEEGLRRRRGSGGEWNRLDQETV
                     AFHTRVREGYLALARAEPDRWAVIDAARGADEVQVDIQAEVEKRLRVKRET"
     gene            complement(5115..6719)
                     /locus_tag="AC162G_03565"
     CDS             complement(5115..6719)
                     /locus_tag="AC162G_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HIR5074925.1"
                     /translation="MPQLRITDDLHVLLDVLPPEIAEAVRRANRSNDLLEVVLDLGRC
                     PEARFVDTEIELSDQEVAQADIDLVVSRIGDFDADNRAGIERTLHRISCIRNRRGNVV
                     GLTCRVGRAVYGTIDVIEDIVRAGKSILMLGRPGVGKTTMLREVARILAEGKPTDRVP
                     NRRVVVVDTSNEIGGDGDIPHPAVGRARRMQVAAPSLQHEVMIEAVENHNPEVIVIDE
                     IGRELEAAAARTIAERGVQLIGTAHGNTLDNILLNPTLSDLVGGIESVTLSDEEARRR
                     GTQKTVLERRAPPTFDILIEIQERDRLLVHHDVAASVDAVLRGRPLQTELRIRGANGE
                     ISKEWVNVPGLAASRERPRHPREIEPQAPAAAPDAAAAPFSPDERGPFRPVRVYAYGV
                     ARNRVEESATRLRVPVYLTDEVGHADVILTVKNYYRKRPRLISDAERRGMPLYVLRAN
                     TTTQIENVLADIFGLSLEESDPYAAAMREAQDAIQKVVAGMRTIDLAPQSAEVRRMQH
                     ELARQANLISHSYGREPYRRVRIYRE"
     gene            complement(6785..7798)
                     /locus_tag="AC162G_03570"
     CDS             complement(6785..7798)
                     /locus_tag="AC162G_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074926.1"
                     /translation="MRSTTVGALRAPTAGIDPFTTMIRPFHLRDVPLVAQLEHEGTPL
                     NAERALTRKPRPLQSALASFFSLSNHGTRTYICRGTLPDRAAKVEGLVQIRPRPSATH
                     GYVTFIAPALSRGGHADGVWAHLLEYASQEAGGMGLHSLIAEAPEDGEEVEVLRRCGF
                     AVYLRQDIFRVEPQRRPAPVQGALRPFEEVDTWAVQQLYFNTAPRLAHLAEGMPRTAP
                     SGARGYVHEEKGELLAYVEVRRGPHGAWINLLVHPDAEARAAHIMSEALSELGSGWEK
                     PIYCGVRRYQEWLRRPLESLGFDLFGSTVLMVKHLAAHIAAGETAPAHAPALERVKIS
                     TPL"
     gene            complement(7795..8379)
                     /gene="pdxT"
                     /locus_tag="AC162G_03575"
     CDS             complement(7795..8379)
                     /gene="pdxT"
                     /locus_tag="AC162G_03575"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HIR5074927.1"
                     /translation="MKIGVLALQGAFIEHEQVLNRLGVETCEVRLPKDLEGLDGLILP
                     GGESTTIGKLAVQYGLIEPLREMARSGKPVWGTCAGMIFMARDAGRDQPLLGLMDVKV
                     KRNAFGRQVDSFEADLQVKDIEGERPFHAIFIRAPLIESVGDGVEVLSRIDRNGDRVI
                     VAARQGRLLATSFHPELSGDDRVHRFFLKLIDKR"
     gene            complement(8380..8868)
                     /locus_tag="AC162G_03580"
     CDS             complement(8380..8868)
                     /locus_tag="AC162G_03580"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5074928.1"
                     /translation="MKPSQGYNLRQATPADIPTLVNHRRRMFEDMAAAGGTPHDLAAL
                     DAMDVGYVAHLSLRLVDGAERAWVIESGERIVASGAVLLSEWLPLPSNLTGRLAYLHS
                     VYTLPEHRRRGLARRIVVTAIEFCRAQGLRRLVLHASREGRPLYESLGFAPTNEMRLV
                     LD"
     gene            complement(8901..9788)
                     /gene="pdxS"
                     /locus_tag="AC162G_03585"
     CDS             complement(8901..9788)
                     /gene="pdxS"
                     /locus_tag="AC162G_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HIR5074929.1"
                     /translation="MDKEVGTVTIKRGLAQMLKGGVIMDVVTPEHAKIAEDAGACAVM
                     ALERVPADIRVQGGVARMSDPELILRIMDTVTIPVMAKCRIGHFVEAQILEAIGVDFI
                     DESEVLTPADEAHHINKHNFKVPFVCGCRNLGEALRRIGEGAAMIRTKGEAGTGDVVE
                     AVRHARAVIGEIRRLTTMADEELMAYAKEIGAPYELVVETKELGRLPVVNFAAGGVAT
                     PADAALMMQLGVDGVFVGSGIFKSGDPARRGKAIVQAVTHYDDPAILAEVSKSLGEPM
                     VGRTVMSIPEPERLAPRGW"
     gene            10092..11063
                     /locus_tag="AC162G_03590"
     CDS             10092..11063
                     /locus_tag="AC162G_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5074930.1"
                     /translation="MTRFVVRRLAIIPIALFLAHFFGFAYAHLARPLRAARNPFLAPL
                     MDSDPLLPAYFGYLQQIPESGLGGVPGSGALVGEVVLQATLASLGLLSIALTLSIIVG
                     WFLGMRAVRTNPPRVARWMTIFSTVGLATPSFYLGTLLIVAMFAYVIGRGPGTGMPFP
                     TQGFGWDIHLVLPVVTLMLRPTAQIAVVISGLLVAELDKQYVVTARSLGHPWKNIRER
                     GALRNIVAPVVLTIAGSARLLMAELIVVEWLFNWPGVGRLLASTLVPTATSAGAGATI
                     HFLDQPLVAALVIVFAALFLIADLIASILVRAFDPRVRAPEVEPAQA"
     gene            11128..13374
                     /locus_tag="AC162G_03595"
     CDS             11128..13374
                     /locus_tag="AC162G_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR5074931.1"
                     /translation="MDNESTNEAECLSSPRGLRRANWSLITGGLLVLLVAVLAIAGPK
                     LAPRDPLEENPVIKVGDQWDARPFAAFTPGFPLGSDALGRDLLSRLLWGIGPAIVLVS
                     IVAAVRLALGLMIGLGAGWSAGRIGRALDAAIGAALTLPVLIVALAAIAAIGVEIGVL
                     AFVIGLSITGWAETAQVVRDQTRIVKGEDYVESARGLGESGLQIVVRHVRRQVLPLAW
                     MLFAFEVSGALMVTAQLGFLGYYVGGDYWYAAGDYSAHRASGMPELGQLLATSSHSFL
                     EDPWGMFAVGSVIFMAILGFNLVGQGMRQRLSSAPASKPTLYARTLGRAGAWIEERIM
                     SPLSGRDRKVLMGASLALGLIAIGAAGISFLNAQAAAQPQGPVADLVIPGEHLWATER
                     HDPWGTLWTPAHGPSAAPKIEWVFEDASGFIGGPAIAADGTLYIASKGGTLHAIDSGG
                     SVRWQAALPTEPVGTPALDAAGNVYVADEEGLSAIAPDGELKWRFRSEKGIAISGPIV
                     APDGTIYYAHGGGFVQAVSPEGQSLWRERARKTAFQSEHPPQISPNGEWVLLENGLLR
                     GVEGSLHVFAGLPTEFSRYLVGADGRTYLLAGHIVSEWRQDGLDARIVQIAEWESRGI
                     SIWVPSDAGITRDQTVWMQYGSARAPTQIAWLDPTGRVLGTLKIPQNPSRAIAVDGDS
                     TIYVCADIQQGHPAATCFAFAPESEEPVWQITLEQGEVVEGGAVVPGRLYVAIREGFL
                     YAIGESQP"
     gene            13389..>13809
                     /locus_tag="AC162G_03600"
     CDS             13389..>13809
                     /locus_tag="AC162G_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013507.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074932.1"
                     /translation="MKKCFLCGTFVTGLLLMACTPSPAATPSATSTPAPTAAAREVVT
                     VESPTAAPPTLALTPTPVSAQAAWTMGKPMIPPYRSEMPAVVLDGMIYVPGGFGGESR
                     LERYDPIADEWEALADMPDGRHHLMAAAHDGYLYVFGG"
BASE COUNT         2305 a         4634 c         4431 g         2439 t
ORIGIN      
        1 gaaagcgcgc cgcgaaacgc ctcaccgtct gcggcgtcag cgcgatctcc ggcacgagga
       61 cgatcgcctg cctgccgcgc tgcagcatcg cgtcgaccgc ccgcatataa atttcagtct
      121 tgcccgagcc ggtcacgccg cgcagcagaa acgccgtagt agcgacttca gtcgctcccc
      181 gacgactgaa gtcgtcacta ctaatcactc cttcgatctg ctcccacacc gcacgctgat
      241 cgtcagtcag cgtcggcgcg tcagtcggca cgaactcttt gcccgccagc gggtcgcgcc
      301 agacttccgc ctcgctcaag gtgatgaatt cgcgctcggc aaggatttgg aggtctgaca
      361 gggagcaatc ggttgcggca tagatatccg agagaagaac cgggccggac ttgtcggcga
      421 gaagacgcag aacagacgcg cggcgcgccg cgacatccgc gcgctccgac agcatcttcg
      481 cttcgatccc ggccggatcg acgaccaggc gggcgaagcg cgccgtcttc gggcgcacgc
      541 tcggcggcag gagaaccggc cggcgcgcga ccacgcctcg gcgcgtcagc gagcgcatcg
      601 cgtcttgcca gggcttgcga tgcagcgcgc gagcgatctg cgcgccccgc agcgggccgc
      661 gccggttgag cagcgcgaag acttccgact gcgtcgcgtt catccgctcg ggcacggaat
      721 cgacggccat cgcatacagc gtatcgaccc ggccggcgat gcccgccggc acgaacaggt
      781 cgatgcattc gttcagcggc gcgagcgtcg cctggctgat ccagcgcgcc aggtcgagct
      841 gcagcggcga caggaccggc gaaggatcca ggagcgattc gatcggcctg gtgcgctcga
      901 ccggcgaaga ctcactcagc gcgacgacga cgcccggcat gcgccgctcg cgcagcggca
      961 cgacgaccag gtggcccggc gcgagccggc cgcgcagatc gtcggggact tcgtagtgaa
     1021 aggcgcgccc ctcttcggat gcgcgcccga tgtgtttcag gatgacgatt tcggcgaaga
     1081 gcgaggcctg cgacagcatc cgtcattcat ccttcgtctt tcgtcgttcg tcgacgcgaa
     1141 tgccaatgcg atcgatgcgc agcccgtcca tgcgctctac gcggaagtgc acgccgtcgg
     1201 cttgcagcga gtcgcctgcg gcgggaatgc gccccaagcg gctgaggacg tagcccgcaa
     1261 tggtatccga gttctggtcg accaggttga ctcccagcgc atcgttcacg tcctcgatca
     1321 gcatcagccc gctcaccgag atgctgccgt cggcgcgcgg cacgaggtcc ggctcttcga
     1381 tggtgaactc atcctgcacg tcgccgaata gttcttcgac caggtcttcg agcgtgacca
     1441 gcccggccgt gccgccgaac tcgtccagca ggattgccat gtgctgcttc tggcggcgca
     1501 tccgtgcaat caggtcgtcg acgcgcacgg tctccggcac gaagagggct tcgcgcatga
     1561 tctcgcgcgc cgtcttggcc agtgccgccc tgcccgcatc ctgctcgatg gattcgtgga
     1621 tcacgcgcac gacgtctttg acgtgcagca cgcccacgac gtgatcggga tcgttttcgt
     1681 agaccgggaa cttggtcagg ctgctgtgcg cagccacgtc gagcgcctct tcgagcgtcg
     1741 ccgtcgcgtc gacggtcacc atttccatgc gcggcaccat gacctggcgc gcgctcgtgt
     1801 cgcgcagatc gaacacggcg tggatcattt cccgctcgtc cggctcgatc aggccgccct
     1861 gctcgctggc ctccgcgatc atcttcaact cctcgatcga gtgcagcgtt ccgtgcgccg
     1921 ccggcctgac gcggatcagc cgcaggagaa aattgcccgt tccgttcatc agccagatga
     1981 aaggccggaa gatcgtctcg gcgatcagcg tcggcccggc gatgaacagc gccgtgcgct
     2041 ccgggttcat cagcgcgatc gatttcggca tcagctcgcc gacgatgacg tgcatgaagg
     2101 tgatgatcgc gaacgcgatg gcggttccgg cgacgatgtg cgccgcctcg ccggcgatct
     2161 cgcccggcag gaatgcgagc agcggctcga tcaggtgcgc cagcgccggc tcgccgatcc
     2221 agcccaggcc caggctggcc agcgtgatgc cgagctgcgt ggcggcgatg aagcgatcgg
     2281 tgtctttgat cgcgtgcttg acggccgccg cgcccggcgt gccgccgcgc accagctctt
     2341 ctacgcgcgt gggccgcacg ctgaccaggc tgaactcagc cgcgacgaag aacccgttcg
     2401 ccagcaccag gacgacgatt gcgatgaggc cgaaaacgct ctcggtcatg tctgcttctc
     2461 tctacctaac ctgggcaggc gcaaggcctg cccctacgtt atacattcca tcgggcgaca
     2521 ccaagcatca caccgataat caaaaaggcg acgaaaagcg ccacgcccgg cagcgcgcgg
     2581 cgcatcgaat tggtatcgac caggcgcggc ctctccggcg catattccac ctgatacatc
     2641 gggcacaaca gccacccaag cacgaggccg ccggcgaagc cgccgatgtg ccccagcatg
     2701 tcgatgcgcg gcaccgtcag cccgaagagg aggttcacac ccacgacggc gacgtagtta
     2761 cccagacgct gtctgcccca cccgccgaag cggtcccggt ggcgcacgaa gaacgcgatc
     2821 atcgtgccga gcaagccgaa cacggccgtc gaggcgccgg cagatagccc gatgtccgcc
     2881 cgaagcgcga agctggctat cgatccggca aggccactca acacatagac gacgacgaaa
     2941 cgcggcgggc cgtacagcgc ctccacgtcg gcgccgagca cgtagatggc gtacgagttg
     3001 aacagcaaat gaacgatgcc aatgtggatg aagttggccg ccaacaggcg ccagtattca
     3061 cccgccgcga cgaagggcgt gaagttcgcg ccgaactgaa tgagaaccac cgggtcctct
     3121 gagccgcccg ccagtgtcat taacagccag acggcgacgt tggccgccag gaatatccac
     3181 atccagcgcg ggcgggacag cggcaggcgc agcgcgaccc tctggcccgg cgcaggcgcc
     3241 cctggcactg cttgcgaggc atccggctga ttcataggct gaccttccgt ggatggacgc
     3301 ggctgtgctc ggcgcgcaca atgccgagca cggcgtccac cgcatcgtcg agcgcgccgt
     3361 tccgattgac gacgacgtaa tcgaactcgg gcaggtgttg cagctcttgg cgtgcggtat
     3421 ccattcgggt cttcaggcta tcgggcgtct cggtgcggcg cgtgcgcagc cggtcggcca
     3481 gctcgccctc gtcgcccgcc atcaggaaga tcagcacggc ttcgggcacg aggcggcgga
     3541 tcgtcatcgc gccctgcacg tccaggcgca tgatcacgtc cgtgcccgag gcgagcgcct
     3601 cgcgcacctg ctgcttggga atgcccttgt agtcgccgta aacgaccgcg tattcgagca
     3661 gctcgtctcg ctcgatcatc gcgacaaatt catccctcga cacgaaatga tagtcccggc
     3721 cttcgacttc acccggccgc ctggggcgcg tgttggccgt gacgacgaaa tggaacggca
     3781 ggttcttttc cttcaggcga gccagcaatg tatccttgcc cgcgcccgac gggccggaaa
     3841 cgacgatgag cagcggatgg gtgggacgcc ggtagaggtc cgtttcgctc aagggttgct
     3901 ccaggtgacg tatatcatta taattgcctg tgacccgaaa ctcaacatct cgccgccgat
     3961 tgggcggaga ttttgtggag cgtgagcgtg cccatctatg aatatcgctg cccgaactgc
     4021 aagaagagag tctctgtctt cttccgcagc ctgtcgagcg tcgatcacga agcggcgcgc
     4081 tgccccaatt gcggcagcgg tggcctggcg cgcctgatgt cgcgcgtgcg cctggtgcgc
     4141 ggctcgtcga agtcgagcgg cgacggtggc gacgacgact tcggcgacga tatgctggat
     4201 agcatcgacg agaacgatcc gcgctcgctt gggcgtttca tgcgcaagat ggcggaagaa
     4261 tccggcgaag acctgccgcc tgagttcaac gaagtcgtgg ggcggctgga gaagggcgaa
     4321 tcgcccgaag acatcgagaa gtccatgccc gaattgggcg atatggcggg cgacgagggc
     4381 gacggcgatt tcggcggcga cgacgagtag attcgtaaaa cgtgatgcgt aaaacgtgat
     4441 aggtgctggt ctatgcctca cgtttcacgt ttcacgcttc acgcgcagcc tcttctccac
     4501 ctctgcctgg atgtccacct gcacctcgtc tgcgccgcgc gccgcgtcga tcacggccca
     4561 ccgatccggc tcggcgcgcg ccagcgccaa atacccttca cgcacgcgcg tatggaacgc
     4621 gacggtctct tgatcgaggc gattccactc gccgccgctg cctcggcgcc ggcgcagccc
     4681 ctcttcggcg tcgagatcca gcagcaaggt caggtcgggg cgaagcccgt ccgtcgcgaa
     4741 tgcgagaatc gcgcggaggg aggccggatc gatcccgcgc ccgtatccct ggtaagccag
     4801 cgagctgtcg gcgtatcgat ccgacaagac gatcccgccg cgctcgaggt gaggccggat
     4861 cacctcgctc accaactgcg cccgcgatgc gcagtacaaa agcagctccg tcaccggccg
     4921 catcgagcgg ttcccaggcg cgtgcagaat cgcgcgcacc tggtcgccga tgcgggtgcc
     4981 gcccggctcg cgcgtcgcga tcacgtcatg cccggcggcg cgcagcctct cggtcagccg
     5041 cgcgacctgc gtcgtcttcc cgctgccgtc cgggccttca aaggtaacaa ataggctcac
     5101 agtgtgtctg aggtctattc gcgatagatg cgcacccgcc ggtacggctc gcgcccgtag
     5161 gagtgcgaga tcaagttggc ctgccgcgcc agctcgtgct gcatgcgcct gacttcggcc
     5221 gactggggcg cgaggtcaat cgtgcgcatg cccgcgacga ccttctggat cgcatcctgt
     5281 gcctcgcgca tcgccgccgc gtagggatcg gactcctcga gcgacagacc gaagatgtcg
     5341 gccagcacgt tttcgatctg cgtggtcgtg ttggcccgca ggacgtacaa tggcatgcct
     5401 cgacgctcgg cgtcgctgat caggcgcggc cgcttgcggt agtagttctt cacagtgagg
     5461 atcacgtcgg cgtgccccac ttcgtcggtc aggtaaactg gcacgcgcag ccgcgtggcc
     5521 gactcctcga cacgattccg agcgacgccg taggcataca cgcgcaccgg tctgaacggt
     5581 cctcgctcgt ccggcgagaa cggcgccgcc gccgcgtccg gcgcagccgc cggcgcctgt
     5641 ggctcgatct cgcgagggtg gcgcgggcgc tcgcggctgg cggccagacc gggcacgttg
     5701 acccattcct tgctgatttc gccgttcgcg ccccgaatgc gcagctcggt ctggagcggc
     5761 cggccgcgca gcacggcgtc cacggaagcc gccacgtcgt gatgaacgag cagccggtcg
     5821 cgctcctgaa tctcgatcag gatatcgaac gtgggcggcg cgcgccgctc gagcacagtc
     5881 ttctgcgtgc cgcgccggcg cgcttcttcg tcggacagcg tcacgctttc gatgcccccg
     5941 accaggtcgg acagggtggg gttgagcagg atgttgtcga gcgtgttgcc atgcgctgtg
     6001 ccgatcaact gcacgccgcg ctcggcgatc gtgcgcgccg ccgccgcttc cagctcgcgg
     6061 ccgatctcgt cgatgacgat cacttccggg ttgtggttct cgaccgcctc gatcatcacc
     6121 tcgtgctgca gggagggtgc ggcgacttgc atgcgccgcg cccggccgac cgccgggtgg
     6181 ggaatgtcgc cgtcgccgcc gatctcgttg gacgtgtcga cgacgacgac gcgccggttg
     6241 ggaacgcggt cggtcggctt gccttcggcc aggatacgcg cgacttcgcg cagcatcgtc
     6301 gtcttgccca caccggggcg ccccagcatc agaatgctct tgccggcgcg cacgatgtcc
     6361 tcgatgacgt cgatcgtgcc gtacaccgcc cgcccgacgc gacaggtgag acccaccacg
     6421 ttaccgcgcc ggttgcggat gcaactgatg cgatgcagcg tgcgctcgat gccggcccgg
     6481 ttgtcggcgt cgaagtcgcc gatgcgcgac acaaccagat cgatatcggc ttgcgccact
     6541 tcctgatcgc tcagctcgat ctcggtatcg acgaagcgcg cctcgggaca ccggcccaga
     6601 tcaagcacga cctcgagcag gtcgttgctc cggttggcgc ggcgcaccgc ctcggcgatc
     6661 tccggcggca gcacgtcgag cagcacatgt aagtcgtcag tgatgcgtag ctgcggcatg
     6721 acattcccca taacgcgcga agcgtaatca cgtgatcacg tgatcacgtg attacgtcat
     6781 cacgctacag cggcgtcgaa atcttgacgc gctccaatgc gggcgcgtgc gccggcgccg
     6841 tttccccggc ggcgatgtgc gccgccagat gcttgaccat caataccgtc gacccgaata
     6901 gatcgaaccc cagcgattcg agcgggcggc gcagccactc ctgatagcgc cgcacgccgc
     6961 aatagatggg cttctcccaa ccactcccca gctcggagag cgcttcgctc atgatgtgcg
     7021 ccgcacgcgc ctcggcgtcg ggatgcacca gcaaattgat ccacgcgccg tgcggcccgc
     7081 gccgcacctc gacatacgcc agcagctcgc ctttttcctc gtggacgtat ccgcgcgcgc
     7141 cggatggagc ggtgcgaggc ataccttcgg ccaggtgcgc caacctgggc gcggtgttga
     7201 agtacaactg ctgcaccgcc caggtgtcca cctcctcgaa cgggcgcagc gcgccctgca
     7261 cgggagcggg acggcgctgc ggctcgacgc gaaagatatc ttgccgcagg tacactgcaa
     7321 atccgcatcg gcgcaatacc tcgacttcct cgccgtcttc cggcgcctcg gcgatcaggc
     7381 tgtgaaggcc catcccgccc gcttcctggc tggcgtattc cagaagatgc gcccacacgc
     7441 catctgcgtg tccgccgcga gacagcgccg gcgcgatgaa cgtcacataa ccgtgtgtgg
     7501 cgctcgggcg cggccgaatt tgcacgagtc cctcgacctt cgccgcccgg tcgggcagtg
     7561 tgccccggca tatatacgtg cgggtgccat ggttgctgag cgaaaagaag gaggccagcg
     7621 cggactgcaa ggggcgcggt ttccgcgtga gcgcccgttc ggcgttgagt ggcgtgccct
     7681 cgtgctccaa ctgggccacc aggggcacgt cacgaagatg aaaggggcgt atcatggttg
     7741 tgaacggatc gattccggcg gtcggcgccc tcagtgcgcc gactgtcgtg cttctcaacg
     7801 tttgtcgatc aacttcaaga agaatcgatg cacgcgatcg tcgcccgaca gctcgggatg
     7861 aaacgacgtc gccagcaggc gtccctggcg cgcggcgacg atcactcgat cgccgttccg
     7921 gtcgatccgg gatagcactt cgacgccatc gccgaccgat tcgatcagcg gcgcccgaat
     7981 gaagatggcg tggaagggac gctcgccttc gatgtccttt acctgcaagt cggcctcgaa
     8041 cgaatcgacc tgccgtccga acgcgttgcg cttcaccttc acgtccatca ggccgagcag
     8101 aggctgatcg cgcccggcgt cgcgggccat gaagatcatg cccgcgcacg tgccccacac
     8161 gggcttgcca gagcgcgcca tctcgcgcag cggctcgatc agcccgtact gcacggccag
     8221 cttgccgatc gtcgtcgatt cgccgcccgg gaggatcagg ccatccaggc cctcgaggtc
     8281 cttcggcagg cgtacttcgc aggtctcgac gccgagccgg tttagtacct gttcatgttc
     8341 gataaaggcc ccttgcagag ccaggactcc tattttcatc taatccaaaa ccagtcgcat
     8401 ctcgttcgtt ggggcgaacc ccaacgattc atagagcggc cggccctcac ggctggcatg
     8461 caggacgagc cggcgcagcc cctgcgcccg gcagaactcg atcgccgtca cgacgatccg
     8521 ccgggccagc ccgcgccggc ggtgttcggg cagcgtgtaa acgctgtgca gataggcgag
     8581 tcgccccgtc aggttgctcg ggagcggcag ccactcggac agcagcaccg cgccgctggc
     8641 cacgatccgc tcgcctgatt cgatcaccca cgctcgttcc gcgccatcga ccaggcgcaa
     8701 gctcaggtgc gcgacgtacc cgacgtccat cgcgtcgagc gcggcgagat cgtgtggcgt
     8761 cccaccggcc gcagccatat cctcgaacat ccggcgtcga tgattcacca gcgtggggat
     8821 gtcggccggc gtcgcctgac gaaggttgta accttgcgaa ggtttcacgc ttcgcgcctt
     8881 cgcaaggttc gccggctcgt ctaccatccg cgcggcgcga gtcgctcagg ctctgggatc
     8941 gacatgacgg tacgtccgac catcggctcg cccaggctct tgctgacttc ggccaggatg
     9001 gccggatcat cgtagtgcgt cacggcctga acgatcgcct tcccacgcct ggccggatcg
     9061 cccgacttga agatgcccga gccgacgaag acgccatcga cgccgagctg catcatcaac
     9121 gccgcatcgg ccggcgtggc gacgccgccg gccgcgaagt tgacgaccgg cagccggccg
     9181 agttccttcg tctcgacgac cagctcgtat ggcgcgccga tctccttggc gtacgccatc
     9241 agctcctcgt cggccatcgt cgtcaggcgg cgaatttcgc cgatcaccgc gcgggcgtgg
     9301 cgcactgctt cgaccacgtc cccggtgccc gcctcgccct tggtgcggat catcgccgca
     9361 ccttcgccga tgcgccgcag cgcctcgccc aggttacggc agccgcacac gaaaggaacc
     9421 ttgaagttgt gcttgttgat gtgatgcgcc tcgtcggcgg gcgtcagcac ttcactttcg
     9481 tcgatgaaat cgacgccgat ggcctccaga atctgcgcct caacgaagtg gccgatgcgg
     9541 cacttcgcca tgaccgggat ggtgactgta tccatgatgc gaaggatcag ctctggatcg
     9601 ctcatgcgcg ccacgccgcc ctgcacgcga atgtcggccg ggacgcgctc cagcgccatg
     9661 accgcacacg cgcccgcatc ctcggcgatt ttggcgtgct ccggcgtcac cacgtccatg
     9721 atcacgccgc ccttaagcat ctgggccagg ccccgcttga ttgtgactgt tccgacttcc
     9781 ttgtccatat tttgctctct ccgcgcccca gcgagtaaag ccgtggggct attaagattt
     9841 taccacgaat cggaaccctc tcaacccatt actatagacc tggcaggtct tctccgacgg
     9901 tattgacatg cgccggagcg caaagacttg gtctgctcgg tgaattccgg cggagatcct
     9961 cggtttctat aaagaagcca gggttctgca tgaaccttct ctggcaattc ggtggtatat
    10021 tgtgtgcacg cgcctgaccg gccgccccgc cttacccgcc gacttcgacc tggctttcag
    10081 gagacgccac tgtgactcga ttcgtcgtcc gccgcctggc catcattccg atcgctttgt
    10141 tcctggcgca cttcttcggg ttcgcctacg cgcacctcgc gcggccgctg cgcgcggcgc
    10201 gcaacccgtt cctcgcaccg ctgatggact cggaccctct gctcccggcc tacttcggct
    10261 acctccagca aatccccgag tcggggctgg gaggggttcc cggttcgggc gcgctggtcg
    10321 gcgaggtggt gttgcaggct accctcgcca gccttggcct gctgtcgatc gcgctcacgc
    10381 tcagcatcat cgtcggctgg ttcctgggca tgcgcgccgt ccgcacgaat ccgccccgcg
    10441 ttgcgcgctg gatgacgatc ttctccaccg tcggcctggc gacgccgagc ttttatctcg
    10501 gcacgctgct catcgtcgcc atgttcgctt acgtgatcgg gcgcgggccg ggcacgggca
    10561 tgccgttccc gacgcagggg ttcggctggg acattcacct ggtgctgccc gtcgtgacgc
    10621 tgatgctcag gccgacggcg cagatcgcgg tggtgatctc cggcctgctc gtcgccgagc
    10681 tggacaagca atacgtcgtc acagcgcgca gcctgggtca cccgtggaaa aacatacggg
    10741 agcgcggcgc gttgcgcaac atcgtcgcgc cggttgtgct gacaatcgcc ggatcggcgc
    10801 gcctgctgat ggccgagctg atcgtcgtcg agtggctgtt caactggccc ggcgttggcc
    10861 gccttctggc ttcgacactc gtgcccactg ctaccagcgc cggcgccggc gcgacgatcc
    10921 atttcctcga ccagccgctc gtggccgcgc tggtgatcgt cttcgctgcg ctgttcctga
    10981 tcgccgacct catcgcgtcg atcctggtgc gcgcattcga cccgcgcgtg cgcgcgcctg
    11041 aggtcgagcc tgcacaggcg tgagtattga gacttccgaa gtctgcaaga cttcggaagt
    11101 ctgaaaagca gcagatggcg tcaacgaatg gacaacgaat caacgaatga agcagaatgc
    11161 ctgtcatccc cgcgtggctt gcggcgcgcc aactggtcac tgattactgg cgggctgctg
    11221 gtgctgctcg tcgccgtgct ggcgatcgcc gggcctaagc tcgcgccacg cgatccgctg
    11281 gaagagaacc cggtgatcaa agtcggcgat cagtgggatg cgcgaccatt cgcagcgttt
    11341 acacccggct ttccactcgg ctcggacgcg ctggggcgcg atttgctcag ccgcctgctg
    11401 tggggcatcg gaccggcgat cgtgctggta tccatcgtcg cggcagtgcg cctggcgcta
    11461 ggcttgatga tcgggcttgg cgcagggtgg tcggccgggc ggatcggccg cgcgctcgac
    11521 gcggcgattg gcgccgcgct cacgctgccc gtgctgatcg tggcgctggc agccatcgca
    11581 gccatcggcg tcgagatcgg cgtgctcgca ttcgtcatcg gcctgtccat caccggctgg
    11641 gcggagacgg cgcaggtcgt gcgcgaccag acgcgcatcg tcaaaggtga ggactacgtc
    11701 gagtcggcgc gcgggctggg cgagtcgggc ctgcagatcg tcgtgcgcca cgtcaggcgg
    11761 caggttctac cgctagcctg gatgctgttt gcgttcgagg tgagcggcgc gctgatggtt
    11821 accgcacaac tgggcttcct gggctactac gtcggcggcg actactggta tgccgccgga
    11881 gactactcgg cgcaccgcgc gagtgggatg cctgaattgg ggcaactact ggcgacgtcc
    11941 tcgcactcgt ttctcgaaga tccatggggc atgttcgccg tgggcagcgt gattttcatg
    12001 gctatcctcg gcttcaactt ggtggggcag ggcatgcgcc agcgtctgtc ctcggccccg
    12061 gcgtccaaac cgaccttgta cgcgaggacg ctgggccgtg ccggcgcgtg gatcgaagag
    12121 cgtatcatgt cgccgctcag cggccgagat cgcaaagtgc tgatgggcgc ctcgctggcg
    12181 ctcggcctga tcgcgatcgg cgcagcaggc atctcgttcc tgaacgcgca ggctgccgcg
    12241 cagccgcaag gcccggtcgc cgacctggtg attccgggcg agcatttgtg ggcgacggag
    12301 cggcacgatc cgtggggaac gctgtggacg cctgcccatg gcccatcggc tgccccaaag
    12361 atcgagtggg tgtttgaaga cgcgagcggg ttcatcggcg ggccggcgat cgcggcggat
    12421 gggacactgt acattgcgtc gaagggcggc acgctgcatg cgatcgactc cggtggcagt
    12481 gtgcgctggc aggcggcgct gcccaccgag ccggtcggca cgcccgccct cgacgcggcg
    12541 ggcaacgtgt acgtcgcgga cgaagaaggg ttgtcggcta tcgcgccaga tggggaatta
    12601 aagtggcgct ttcgatccga aaagggcatc gccatatcgg ggccgatcgt cgcgccggat
    12661 gggacaatct attacgcaca cggcggcggg ttcgtgcagg ccgtctcgcc ggagggccaa
    12721 tcgctgtggc gagaacgcgc gcgtaagacg gcgtttcagt ccgagcatcc gccgcagatc
    12781 agcccaaacg gcgagtgggt actcctggag aacggcttgc tgcgcggcgt cgaaggatcg
    12841 cttcatgtct tcgccggact gccgaccgag ttctcacgct acctcgtcgg cgcggacggc
    12901 cgcacctatc tactggccgg gcacatcgtc agcgagtggc ggcaagatgg gttagatgcc
    12961 cggatcgttc aaatagccga gtgggaatcc cgtggcatca gcatctgggt gccgagcgac
    13021 gccggcatca cccgcgacca gaccgtctgg atgcagtatg gctcggcccg agcgccgacg
    13081 caaatagcct ggctggaccc gaccggccgc gtgctcggca ctctgaaaat cccgcagaac
    13141 cccagccgcg cgattgcggt cgatggcgat tccaccatct atgtctgcgc cgacatccag
    13201 caaggtcacc cggctgctac ctgtttcgcg ttcgcgcctg aatccgagga acctgtctgg
    13261 caaataacgc ttgagcaagg tgaagttgtc gagggcggcg cggttgttcc gggacgactt
    13321 tacgtcgcga ttcgagaggg ctttttgtat gcgatcggcg aaagccagcc gtgaaacgag
    13381 cgacagcgat gaaaaagtgc tttctctgcg gaacatttgt aacgggactg ctgctgatgg
    13441 cgtgcacgcc ttcgccagcg gcaacacctt cggccacatc gactcccgcg ccgaccgctg
    13501 cggcgagaga agtcgtcaca gtcgaatcgc cgaccgctgc cccgcctaca cttgcactga
    13561 cgcccacgcc agtctcggcg caggcggcgt ggacgatggg caaacccatg atcccgccgt
    13621 atcgatccga aatgcccgcc gtcgtgctgg atggcatgat ttacgtgccc ggcggcttcg
    13681 gcggcgagtc acggctggaa cgctacgatc ccatcgccga cgaatgggaa gcgctggccg
    13741 atatgccgga tggtcgccat cacctcatgg cggctgcgca cgacggatac ctgtacgtgt
    13801 tcggcggct
//
LOCUS       DBNHXB010000093         7000 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2868671, whole genome shotgun sequence.
ACCESSION   DBNHXB010000093 DBNHXB010000000
VERSION     DBNHXB010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2868671"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="AC162G_03605"
     CDS             <1..555
                     /locus_tag="AC162G_03605"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5074933.1"
                     /translation="GAVIAICSSAMPETCVQIAAVAVQKGVGVVDAPVARGVRGAETG
                     DLTVFAGGEAEDIDKCRPVFESFAKYVFHMGSVGSGQITKTVNNLIHWVEVVGIYEAL
                     RLGAAHGVHPNKLRPALLAGSVDSRTLRELHLVGMAWPHKDMENALRLAEASHTPLPL
                     MARVGELVAKMSKEDLRGLFVEEA"
     gene            552..1256
                     /locus_tag="AC162G_03610"
     CDS             552..1256
                     /locus_tag="AC162G_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012804.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR5074934.1"
                     /translation="MTTDTLRAELTEKLVTCIRLMAMEGLLDFNGHVSVRLPDTQLLI
                     NARYSTRAGVTPDQIVIADMAGKLIEGDDQPPSEISIHTEIYRARPDVNCVAHLHPQY
                     ATVFTIAGVPLVPVYIVASLFGQEGIPVFDDPRLIRTREDGQAIAAALGHKPALLLRA
                     HGAVAVAADIEGVFAVAFAMEDNAKKAIWASALGKPRPLTPAEMEQYGAPVSDRGTKR
                     KIWNFHVQKAHATGWL"
     gene            1335..1802
                     /locus_tag="AC162G_03615"
     CDS             1335..1802
                     /locus_tag="AC162G_03615"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5074935.1"
                     /translation="MTNDHQAPDEVKELEQFRAGHPNVGMHFKNLKTFPVHYRRLRGT
                     GGAWFDLSGNHTIEAHVSEIPPGGHNKKHRHMNEAIIYILSGRGYSTIQVEGQPEERY
                     DWEAGDMFSPPLNAWHQHFNSDPEQPARYLAVTNIGLMTKMGAFSKEQAPGQE"
     gene            1852..2310
                     /locus_tag="AC162G_03620"
     CDS             1852..2310
                     /locus_tag="AC162G_03620"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5074936.1"
                     /translation="MTSSSHLDEIAEREAFRKEYLQEGIFFKNLKTFPVVKRKLRGSA
                     SAHFGLAGNRTIDAHISELPPGGHNNMHRHINEAIIHILSGRGYSIIEEDGKEPVRID
                     WEEGDLFSPPLNAWHQHFNADSEKPARYLAITNVPLMTAMGTFVKEQRGR"
     gene            2428..3549
                     /locus_tag="AC162G_03625"
     CDS             2428..3549
                     /locus_tag="AC162G_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5074937.1"
                     /translation="MKARVSRPTRLFQALAGLIVLALLAAGCGPAATPTAAPPTQAPP
                     TQPPPTQAPPTQPPEPAPTQELTLEEAAQAEGGQLMVYTSMNIDDLEVVLAAFKEAYP
                     FVAPEYYRASGEDVIAKAVTEAQAGQHFADVFETNAFEVYRLMQEGLLAPFDAPERAA
                     YPDNAKDPEGYWTVDRINLVVIAYNTELVDAADAPKTHQDLLDPKWQGQIAVEPNDVE
                     LLADMVGAWGEDEAVAFWEGIAAQEPRLVDGHTELAEFMVAGEFAISPTVYGHRVVKL
                     KGDGAPIEWVITDPVFAFTQLLALANDAPHPATGKLFVNWLLSEEGQNVIKSLDRVPA
                     RPGVDDLLEGMNPYYTIPSLAEKYNDYLEAWKSLGLSQE"
     gene            3698..5446
                     /locus_tag="AC162G_03630"
     CDS             3698..5446
                     /locus_tag="AC162G_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019526852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5074938.1"
                     /translation="MIATKTEAGRARRWPARLKLPRLTDARWWIMGGTILFVFYLAGV
                     PLLLLLWGSLKIGGPTEVSALTLENFREAYSDPRSYTLLLNSVVYAGGTCLFSLILGT
                     SLAFVIERTNTPLKSLFTAMALVPLIVPGILHTIAWILLLSPRIGVVNRALMGLLGLA
                     EAPFNIYSLGGMIWVEGLHLSPLVFVIMAAAFRSMDPALEEAALTSGAGVPATLRRVT
                     LQLARPAIASTMLIMFIRGLESFEVPALIGLDAGVRVFTSRIFLALKEFPPNYGLAAA
                     FAVGLLLISVIGILLYNRLTANRGQFATVTGKGFRPHLIDLGKWRYVTSLVFVLYFLF
                     LVGLPLLILLWTSLNRFYTIPALDKLGGLTLDNYAFVMNMGRARTSLVNSLILSVSSA
                     TVVMLVTAVIAWITVKTRWPGRVALDVVTFLPITIPGLVLGVSLIWMYLVLPIPIYGT
                     LWILLLAYITRYMPYGIRANSASMVQIHDELEEAASVAGSSWLQTFRRVTLPLLKPGL
                     IAGWIYVVVVSFRELSSSILLYSSKSIVLSILIFDLWDGGQFPIVAALSVMMVLILVV
                     LVAIAYRLGARFGVRE"
     gene            5491..6678
                     /locus_tag="AC162G_03635"
     CDS             5491..6678
                     /locus_tag="AC162G_03635"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR5074939.1"
                     /translation="MNSRWLTVLSLAVVLGLGWSFARGVNRPPFPQPATFSTQITTPL
                     AIEGLTGDHTNNLYTIGRNAGAGVSCPVWRINLANPSLVVVGYIPAPSATGQCSPSGV
                     AFDAAGDLFAADGTQVLRLTPNAATPPTATVFASGVPGTNGLAFDREGNLWTGDGTTG
                     QGRVWKITPDGVVTEVFRIQPMRNSAALGGVVAGDGVGRQARTFPPGTLANVAGGQDL
                     VANGLAFDREGDLFVADTARGAIWKIEFDPQGNVKSPMGCDTTFTPNTLCLDAILVTH
                     PLLDGVDGIALDQLGNIWGTANERNAVVVATITGTVAEVFRNAPDAASNLRNTGPLEF
                     PTSPFLSDKTLCASNSDGNRRDNSPSSAGEIGGAGQPRGKITCMDQDLLIPGLPLPVD
                     LSP"
     gene            6741..>7000
                     /locus_tag="AC162G_03640"
     CDS             6741..>7000
                     /locus_tag="AC162G_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5074940.1"
                     /translation="MVIVDDLVKTFESKGHESVPAVRGVSFEVPEGKFFTLLGPSGCG
                     KTTTLRTIAGLERPEAGEIRINGRVVCSPAQGIFVPPNERDI"
BASE COUNT         1351 a         2150 c         2179 g         1320 t
ORIGIN      
        1 ggggcggtga tcgccatttg cagttcagcc atgccggaga cgtgcgtcca gatcgccgcc
       61 gtcgccgtac agaaaggcgt tggggtggta gacgcgccgg tggcgcgagg cgtgcgcggc
      121 gctgagaccg gcgacctgac ggtattcgcc gggggcgagg ccgaagatat cgataaatgc
      181 cggccggttt ttgagagttt cgccaaatac gtctttcata tgggatcggt tggcagcggg
      241 cagatcacca agacggtcaa caacttgatc cattgggttg aagtggtggg catctacgag
      301 gcgctgcgcc tgggcgcggc gcatggcgtc catcctaaca agttgcgccc ggcgctgctg
      361 gccggcagcg tggatagtcg taccctgcgg gagttacacc tggtcggcat ggcctggcca
      421 cacaaagata tggaaaatgc gctgcgcctg gccgaagcga gccacacccc actgccgctc
      481 atggcgcgtg taggcgaact ggtcgcgaag atgagcaaag aagacctgcg tgggctattt
      541 gtcgaggaag catgacgacc gacacgctcc gcgccgaact cacagaaaag ctggtcacgt
      601 gcatacggct gatggcgatg gaggggctgc tggacttcaa cggccacgtg agtgtgcggc
      661 tgccggatac acaactgttg atcaacgcgc gctactcgac gcgcgccggt gtgacgcccg
      721 accaaatcgt gatcgccgat atggcgggga aactgatcga aggcgacgac cagcctccca
      781 gcgaaatcag catccacacc gagatctatc gtgcccggcc ggacgtgaat tgtgtagctc
      841 acctgcatcc gcagtacgcc acagtcttta cgattgcggg cgtgccgttg gttcccgtat
      901 acatcgtggc cagccttttc ggccaggagg gcattcccgt cttcgacgac ccgcgcctga
      961 tccgcactcg ggaggacggt caggccatcg cggccgcatt gggtcacaag ccggcgctgc
     1021 tcctgcgggc gcacggcgcg gtagccgtgg ccgccgacat cgagggtgtt tttgccgtag
     1081 ccttcgccat ggaagacaac gcgaagaagg caatctgggc ctcggcgctg gggaagcccc
     1141 ggcccttaac gccggccgag atggagcagt acggcgcgcc agtcagcgat cggggcacca
     1201 agcgcaaaat ctggaatttt cacgtgcaga aagcgcacgc gacaggctgg ctatagtggt
     1261 tgttcggttg gcagttgcac tgccaaccgc cgcagcaact gcggcgggaa cttcgcaaag
     1321 gaggcaaacg agcgatgaca aatgaccatc aggcaccaga tgaggtcaaa gagctggagc
     1381 agttccgggc cggacatccc aacgtgggca tgcacttcaa aaatctgaag accttcccgg
     1441 tgcactaccg ccggctgcgc ggcacgggcg gcgcgtggtt cgacctctcc ggcaaccaca
     1501 ccatcgaggc acacgtcagc gagattccgc cgggcggtca caacaagaag caccgtcaca
     1561 tgaacgaggc gattatctac atcctctccg ggcgcggcta ctcgaccatc caggtggaag
     1621 gccaaccgga agagcgctac gactgggaag cgggcgacat gttctcgccg ccgctcaacg
     1681 cctggcatca gcacttcaac agcgatcccg agcaacccgc gcgctacctg gcggtgacga
     1741 atatcggcct gatgaccaag atgggcgcgt tcagcaaaga acaggcaccc ggccaggaat
     1801 agcgtcacac ggattttggc acggattgca cggataaaaa ggattggcaa catgacatcg
     1861 tcttcgcatc tggatgaaat cgccgagcgc gaggcgttcc gcaaagagta cctgcaagag
     1921 ggcattttct tcaagaacct gaagaccttt ccggtcgtca agcgcaaact gcgcggttcg
     1981 gcgagcgcgc acttcgggct ggcgggcaac cgcacgatcg acgcgcacat cagcgaattg
     2041 ccacccggcg gacacaacaa catgcaccgc catatcaacg aggccatcat ccatatcctg
     2101 tcaggccgcg gctactcgat catcgaagag gacggcaaag agccggtccg gatcgactgg
     2161 gaagaaggtg acctgttctc gccgccactt aatgcttggc accagcattt taacgccgac
     2221 tccgagaagc cggcccggta cctggccatc accaacgtgc cgctgatgac ggcgatgggc
     2281 acatttgtca aggagcagcg cgggcgctga gcacccgcac gagcagacac gcctgcggaa
     2341 gaggaggtgc attggacgtt taccgaccca tgacgtgaat atcggcagcc atttgcagca
     2401 gtatcttttc caaggaggag agaaacgatg aaagcacgtg taagcaggcc aacgaggctc
     2461 ttccaggctc tggcagggct aattgtgttg gcgcttctgg ccgccggctg cggaccggca
     2521 gcgacgccga cagccgcccc tcccacccag gctccaccca cccagccgcc gcccacccag
     2581 gctccgccta ctcagccgcc agagccagct ccgacccagg aactgacgct ggaggaggca
     2641 gcccaggccg aaggcggcca actcatggtg tacacctcca tgaacatcga cgacctggag
     2701 gtagtgctgg ctgcgttcaa ggaggcctat ccctttgtcg cgccggagta ctatcgggcc
     2761 tcgggcgaag acgtgatcgc gaaagccgtg accgaggcgc aggccggcca acacttcgcc
     2821 gacgtgtttg aaaccaatgc cttcgaggtc tatcggctca tgcaggaagg gctgttggcg
     2881 ccgttcgatg cccccgagcg cgcggcctat ccggacaacg ccaaggatcc ggaaggctac
     2941 tggaccgtgg atcggattaa cctggtggtc atcgcctaca acaccgaatt ggtcgatgcg
     3001 gccgatgcgc cgaagacaca ccaggacctc ctcgatccca agtggcaggg ccagatcgcc
     3061 gtggaaccca acgacgtcga gttgttggcc gatatggtcg gcgcctgggg cgaggacgaa
     3121 gccgttgcct tctgggaagg catcgccgcc caggagccgc gcctggtgga tgggcatacg
     3181 gagctggccg agtttatggt agccggtgag ttcgccatct cccccaccgt ttacgggcat
     3241 cgcgtcgtga aactgaaggg cgacggcgcg ccgattgagt gggtgatcac cgatccggtc
     3301 ttcgctttca cccagttgct ggcgctggcc aatgacgcgc cccatccagc caccggcaag
     3361 ttgttcgtca actggctgct gtcagaagag ggccagaacg tgatcaaaag tctggatcgc
     3421 gtcccggcgc ggcctggcgt cgacgatttg ctcgaaggga tgaacccgta ctacaccatc
     3481 ccgtctctgg ctgagaagta caacgactac ctggaggcct ggaaatcgct ggggctgagc
     3541 caggagtaaa cgccgcagtc ccgccggcac gctccgcagc cggtcacctg ggacgtagcc
     3601 gcaaggtgcc gcgtgctgtg tcctggtaag gaacaatagg gcaatcgcat cttctgcaca
     3661 atcaagatgc gattgccctg caaggaacaa caggcccatg atagccacca agaccgaagc
     3721 gggccgggcc agacgctggc ccgcccgctt gaagctaccc cgcctgaccg atgcccggtg
     3781 gtggatcatg gggggcacga tcttgttcgt gttctacctg gccggcgtgc cgctgctgct
     3841 gctgctgtgg ggcagcctga aaatcggcgg cccgacggaa gtcagcgccc tgacacttga
     3901 gaattttcgt gaggcctata gcgacccgcg ctcgtatacc ctgttgttga actcggtggt
     3961 ctacgcggga ggcacctgcc tcttttcgct catcctgggc acgagcctgg ccttcgtcat
     4021 cgaaagaacg aacacgccgc tcaaaagcct gttcacggcc atggcgctgg tgcccctgat
     4081 cgtccctgga attcttcaca cgattgcctg gatcctgctc ctcagcccaa gaatcggggt
     4141 cgtcaatcgg gcattgatgg gcctgctggg attagcggaa gcgccgttca acatctatag
     4201 cctgggtggc atgatctggg tcgagggcct gcacctctcg ccgctggtct tcgtcatcat
     4261 ggccgccgcc ttccgctcga tggacccggc actcgaagaa gcggccttga cttccggcgc
     4321 tggcgtgccg gcgacgctcc gccgcgtaac cttgcaactg gcccggccgg ccattgcctc
     4381 gacgatgctg atcatgttca ttcgtggact ggaatctttc gaggtgccgg ccctgatcgg
     4441 cctggacgcc ggcgtgcgcg tgtttacctc gcgcatcttt ttggccttga aggagtttcc
     4501 acccaactac ggcctggcgg ctgccttcgc cgtcgggctg ctgctgatca gcgtgatcgg
     4561 catcctgctc tacaaccgct tgacggccaa tcgtgggcag ttcgccaccg tcacgggcaa
     4621 aggttttcgg cctcacctga tcgacctggg aaagtggcgt tacgtgacca gcctggtctt
     4681 cgtgctgtac tttttgttcc tcgtcgggct gcctttgctc atcctgctgt ggacgtcgct
     4741 gaatcgtttt tataccatcc cggcgctgga caaactcggg gggttgaccc tggacaacta
     4801 tgctttcgtc atgaatatgg ggcgcgcccg cacttccctg gtcaacagtc tcatcctgag
     4861 cgtcagcagc gcgacggtcg tcatgctcgt gacggcggtc atcgcctgga tcaccgtcaa
     4921 gacccgctgg ccggggcgcg tggcgctgga cgtggtgacg ttcctgccga tcacgatccc
     4981 cggcctggtg ctgggcgtca gcttgatctg gatgtatctc gttctcccta tcccgatcta
     5041 cggcacgctc tggattctgc tgctggccta catcacccgt tacatgccgt acggcatccg
     5101 cgccaattcg gcgtcgatgg ttcagattca cgacgaactg gaggaggccg catcggtcgc
     5161 cggcagctcc tggctgcaaa cgttccggcg cgtcaccttg cctctgctca aacccggctt
     5221 gattgccggc tggatctacg tggtcgtcgt ttcgttcagg gaattgtcca gctcgattct
     5281 gctttacagc agcaagagca tcgtgctttc cattttgatc ttcgacttat gggatggagg
     5341 gcaattcccg attgtcgccg cgttgagcgt gatgatggtc ttgatcttgg ttgtcctggt
     5401 cgcgatcgcc taccgattgg gcgctcgttt cggtgttcgt gaatagtccg ttgggccaat
     5461 ccgagtcaat acgaacagga ggctgagcaa atgaacagca gatggttgac tgtcctctcg
     5521 ctggccgtgg ttctaggtct cggctggtcg ttcgctcgcg gtgtcaatcg gccaccgttc
     5581 ccgcagccgg cgaccttttc cacccagatt acaacgccgc tggccattga aggactgacc
     5641 ggagaccata ccaacaattt atatacgatc gggcgaaacg ccggggctgg agtttcttgc
     5701 cccgtctggc gcatcaattt agccaaccca tctttggtgg tagtcggcta catcccggct
     5761 ccctccgcca cgggccaatg cagcccgtcg ggagtcgcct tcgacgcagc aggcgatctg
     5821 ttcgccgccg atgggacgca agtcctcagg ttgacaccca acgccgcgac gccccccacc
     5881 gcgacggtct tcgcttctgg cgttccgggc acgaacggcc tggcgttcga ccgcgagggt
     5941 aatctctgga ctggagatgg cacgacgggg caggggaggg tctggaagat tacacccgac
     6001 ggcgtcgtca ccgaagtctt cagaattcag ccgatgcgga acagcgccgc gctcggcggg
     6061 gtggttgccg gcgatggcgt cgggcggcag gccaggactt tcccgccggg gacgctggcg
     6121 aatgtggcgg gcggacaaga cctggtcgcc aacggcttag ccttcgatag agaaggcgat
     6181 ctcttcgtgg ccgacaccgc ccgcggcgcg atctggaaaa tcgagtttga cccacagggg
     6241 aatgtgaaaa gcccgatggg ttgcgatacc accttcacgc ccaacacgct gtgcctggat
     6301 gctattctcg tcacacatcc gttgttggac ggcgtggatg gcattgccct ggaccagctt
     6361 gggaatatct ggggcaccgc caatgaacga aacgcggtgg tggtggcgac tataacaggc
     6421 acagtcgcgg aggtcttccg caacgcgcca gacgcagcgt cgaatttgcg gaacaccggg
     6481 cctttggaat tcccgaccag tccattcctc agcgataaga ccttgtgtgc ctccaattca
     6541 gatggtaatc gccgggacaa ctctcccagt tcggccggcg agatcggcgg cgcgggacaa
     6601 ccgcgcggga aaatcacctg tatggatcag gacctgctca ttcctggcct gccgctgcct
     6661 gtcgatctgt cgccctgatt gggcgtcgca ggcccaaggc accggcagag gaacggatgt
     6721 gatctgatac gcgagggcac atggtcattg tcgacgatct cgtcaagacc ttcgagagca
     6781 aggggcacga gtccgtccct gccgtgaggg gcgtgagctt cgaggtcccg gaaggcaagt
     6841 tcttcacgct gctcggcccg agcggctgcg gcaagacgac cacgctgcgc acgattgccg
     6901 ggctggagcg ccccgaagcc ggcgaaattc gcatcaacgg gcgggtcgtc tgctcgccgg
     6961 cgcagggcat cttcgtgccg ccgaacgagc gcgacatcag
//
LOCUS       DBNHXB010000094         7192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2198309, whole genome shotgun sequence.
ACCESSION   DBNHXB010000094 DBNHXB010000000
VERSION     DBNHXB010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7192
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2198309"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="AC162G_03645"
     CDS             complement(<1..477)
                     /locus_tag="AC162G_03645"
                     /inference="COORDINATES: protein motif:HMM:NF010248.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HIR5074941.1"
                     /translation="MGRTLLLTGRPGAGKTTIVKHIAQALGDRAGGFYTEEILGPGGR
                     KGFRVIALDGQQTTLAHVDIRSRSKVGRYGVDVAGFERVGVAALQRAIDGAEIVIFDE
                     IGKMELFSSQFKSAVIKAIDCGKPVIATAMIAAEPWVDALKRMPGVRTVEVTKQNRG"
     gene            535..1887
                     /locus_tag="AC162G_03650"
     CDS             535..1887
                     /locus_tag="AC162G_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR5074942.1"
                     /translation="MTHDAHAAVPSALLPAPFGRGLRRASPGGVGCVARSHVLVYNPT
                     HVTRSERMAQGQGLSRTLIVLLTIAVSIYLLEKLGAFVAALGNVVLLLALSWLLAFVL
                     QPVVNWFDRGIVPRGVLRWAHARAGDVWGERLERLNLPYGLAVAVVFLLVLAALIFVV
                     LELIPVVIDQIAQIAHSLITITEDLPGAFQRVADWLNEVRQRLVVDLRIDPAAIPLPS
                     PEDLIAEVGRLVAGLGQLALGLVAGFVFFLTQLVLVFLIAAYILIDGRKLGRDILQVL
                     PDRLAGDIRVAYSTVDRTFGGFVRGMLLQALLYGSVVTASMLLFGLSYALVVGVATGL
                     LMLIPYVGGIIGLALPLLAGLIQSSPNTLPLVLVLFIFQVLLFNLIMPRILSQALRVP
                     TLFVFVALIVAAQLIGVWGLLFGVPLAAALYSIALALLRRIKHYAEAHAPSEPGLTDG
                     "
     gene            1880..2740
                     /locus_tag="AC162G_03655"
     CDS             1880..2740
                     /locus_tag="AC162G_03655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5074943.1"
                     /translation="MAERRCAGSVELRGSDPNAGEPFFFAGGSIGCVLLHGFTAAPRE
                     MRPMGEFLNERGITVHGVRLAGHGTCPEDLARTTWRDWYTSALDSARGLRGRCRAVFT
                     CGLSLGGALSLLLAADKEVDGAIAINTPLRPHDKRLKYARFLAPFKPYTPKGLANLYD
                     PAALADHADYFRVPTRAAAELYRLTRVLEHALPCVAAPVLVIQSRLDRVVPPEDGQTI
                     FDRIATSDKRLVWLARGGHIATEDYDKEIIFEEALHFIEAHTPAKRAVQLPTSNLQPP
                     TSNLQHHDTR"
     gene            2727..3968
                     /locus_tag="AC162G_03660"
     CDS             2727..3968
                     /locus_tag="AC162G_03660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5074944.1"
                     /translation="MTRDNKIVTISLFLWGIGESMWFYIRGLYLEGLGAMPQEVGLAL
                     GIAALTHAAAYVLGGVLADRFDRKFLLLAGWLTGVVAPPLMALAGDWRAFMIGFSVYN
                     VSGFVVPVIDTYVTQAAGRAPLERVLPVVFAGYWVGSVLGPQVGKALLTMMDERGILL
                     VSSLVFVASTVVIAFVHVQPAARLTLSGAGLRHSRVGPVQALRPALSFFVYLFAIFFS
                     MALGAQLIPNYLLRLGWRSIDVSGIVSALPLGIAVLTVAVGHFAAGRNRRGLLASQAL
                     VLAGIAAFAFGAPAWGGFAVAGYLLLGGFDAARQQALARMPRYVRQEQRGLAFGMAST
                     VGEGAIAAASVIGGVLFALDPALPFYAGLMLIPAGMALTLRLRRPQTAVAADVTAAHA
                     GATVALSPGVRSPTADERTCG"
     gene            3961..4509
                     /locus_tag="AC162G_03665"
     CDS             3961..4509
                     /locus_tag="AC162G_03665"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5074945.1"
                     /translation="MGDHRKAGNDLSIRENLMWEGRTLTATWLPAPFMPALDQVTQAS
                     GVCFTGSGKIVLVAGHTGKWALPGGHVEPGETLEQTLAREVLEEACAIVTQSEYLGAQ
                     EVSDPDNPTGPRVYYQARFWARVELKRFEPRFERMQRTLIAPSAFQSTLRWRTERIAQ
                     AILDAALAAETRYRSRPRKSAS"
     gene            4634..6814
                     /locus_tag="AC162G_03670"
     CDS             4634..6814
                     /locus_tag="AC162G_03670"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HIR5074946.1"
                     /translation="MRWTRRDFLRALAVTLGAAAPPPDGYKIYLPLVTRSSAYAADLG
                     GGVWVIGNDLVEQRIAFIGGGLFTRSFLHKASGRDWCASASSPVFFLEEEEAELTSVD
                     GWVHIESAALNFGDGAKLTVTMQHASRPIRAHAHFHVWSDAPVVRSCTEYENIGAGAR
                     QLQAQSFCALTLGAAGALEVVWVAPFSWLWDRTDDSFVTHRETLQNPGQERHLVMGPY
                     AAEDVGGYNVVTYRPSCGWFAFHQKTLGAGLFGGVEWSGACEAHLSLNDSGQGRIGIG
                     HWRPAFTHAAGPGERVTSPVAFVGPFEGAPDEAAHLTHRLAKAHYIPARPSIRVPAGA
                     EFPYVMADTWGYGPNIDEARLGVFVDRAAEIGVEALTIDEGWEARIGDWVSHPARFPS
                     GLRAMVDTIHSKSMAAGLWFAFANVDPDSAVARAHLDWLATRGGSPIGGSFNTQVLCL
                     SHPDARAWVAAEFDRIVAEFDVDALVQDFETIARCDNPAHAAWHQAVDSEYRNVLALW
                     ELIDGVRARHPNVMIENNWSGGRVMDFGMLRRYDTSVCDDYNQAARNRGASFGVTHFF
                     PPTFVSKYMGAEALPFDYQTRSYFFGGPWNLMTDLPAQDAQMLAGAIGAYKELRELIR
                     DGRVVHLLAPSFSGTGPNRYQISWDAIQAVEPGRNRAVLLIGRGLAGPGTYNVKPQGL
                     NPTKVYALAASSGANYGSRSGADLMANGIDVSLPERTHETIVLA"
BASE COUNT         1110 a         2294 c         2402 g         1386 t
ORIGIN      
        1 tccccgattc tgcttcgtca cctcgaccgt cctcacaccc ggcatgcgct tcagcgcgtc
       61 gacccatggc tcggcggcga tcattgccgt ggcgatcacc ggcttgccgc agtcgatcgc
      121 cttgatcacg gcgctcttga actgagacga gaacaattcc atcttgccga tctcgtcgaa
      181 gatcacgatc tccgcgccgt cgatcgcgcg ctgcagcgcc gccacgccga cccgctcgaa
      241 gccggccacg tctacgccgt aacgccccac cttcgatcgc gatcgtatgt cgacgtgcgc
      301 caacgtcgtc tgctgcccgt cgagcgcgat gacgcggaaa cccttgcgcc cgcccggccc
      361 gaggatctct tccgtgtaga agccgcccgc gcgatcgccg agcgcctgcg cgatgtgctt
      421 gacgatcgtc gtcttgccgg cgcccggcct gccggtgagg aggagagtcc gtcccatgca
      481 caacttcaag ctccaaaggt tcaaacgcac gccagtatat catgagccgt ggtgatgaca
      541 cacgacgctc atgccgccgt cccttcggct ctgctccccg ctcctttcgg tcggggactt
      601 cgcagggcaa gccccggcgg cgttggctgc gttgcccgga gtcatgtcct cgtgtacaat
      661 ccaacgcatg tcacgcggag cgagcggatg gcgcagggac agggattgag ccgcacgctg
      721 atcgtcctgc tgacgattgc cgtgtcgatc tacctgctgg agaagctggg cgcgttcgtg
      781 gccgcgctgg gcaacgtcgt tctgctgctc gcgctctcgt ggctgctggc cttcgtgctg
      841 cagccggtgg tgaactggtt cgaccgcggg atcgtgccgc gcggcgtgct gcgctgggcg
      901 cacgcgcgcg ccggtgatgt ctggggcgag cgcctggagc gcttgaatct gccgtatggg
      961 cttgccgtgg ccgtcgtgtt cctgctggtg ctggccgccc tgatcttcgt cgtgctcgag
     1021 ttgatcccgg tcgtgatcga ccagattgcc caaatcgctc acagccttat cacgatcacc
     1081 gaagatcttc ccggcgcttt tcagcgcgtt gccgactggc tgaacgaagt gcgccagcgc
     1141 ctggtcgtcg acttgcggat cgatccggct gctatcccat tgccatcacc agaagacctg
     1201 attgccgaag tggggcgtct cgttgctggg ctaggccaac tggcgctcgg cctggtggcc
     1261 ggtttcgtct tcttcctgac gcaattggtg ctggtcttcc tcatcgccgc ttatattctg
     1321 atcgacggcc gcaaactggg gcgggatatc ttgcaggtgc ttcccgatcg cctggccggt
     1381 gacatccgcg tcgcgtacag cacggtcgat cgcaccttcg gcggattcgt gcgcggcatg
     1441 ctgctgcagg cgctgttgta cggcagcgta gtgacggcgt cgatgctgct gttcgggctg
     1501 tcctacgcgc tcgtggtcgg cgtcgcgacc ggcctgctca tgctgatccc gtacgtgggc
     1561 gggatcatcg ggctggcgct gccgctgctc gcaggtctga tccagtcctc gcccaacaca
     1621 ttgccgctcg tcctggtgct cttcatcttc caggtgcttc tgttcaatct gatcatgcca
     1681 cgcatcctga gccaggcgct gcgggtgccc acgctgttcg tgttcgtcgc gctgatcgtc
     1741 gccgcgcagt tgatcggagt gtggggattg ctgttcggcg ttccactggc ggcggcgctg
     1801 tactcgatcg cgctggcgct gctgcggcgg atcaagcatt acgccgaagc gcacgcgccg
     1861 agcgagccag gcctgacgga tggctgagcg ccgatgcgca ggctccgtcg aattgcgcgg
     1921 ctcggatccc aatgcgggcg agccgttctt cttcgcgggc gggtcgatcg gctgcgtgct
     1981 gctgcacggc ttcacggccg cgccgcgcga gatgcgcccg atgggggagt tcctgaacga
     2041 gaggggcatc accgtccacg gcgttcgcct cgccggtcac ggaacctgcc ccgaagacct
     2101 ggcccgcacg acctggcgtg actggtatac ctctgcgctc gactcggcgc gcggtttacg
     2161 agggcgctgc cgcgcggtgt ttacctgcgg cttgtcgctc ggcggcgcgc tgtccctgtt
     2221 gttggcggcc gacaaagagg tcgacggcgc gatcgcgatc aacacgccac tgcgccctca
     2281 tgacaagcgc ctgaagtatg cgcgcttcct ggcgccgttc aagccctaca cgccgaaggg
     2341 gctggcgaat ctgtacgacc cggctgcgct cgccgatcat gccgactact ttcgcgttcc
     2401 cacacgcgcg gccgcggagc tgtaccggct gacgcgagtt ctggagcacg ctttgccctg
     2461 cgtcgccgcg ccggtgctgg tgatccagtc gcgactcgat cgggtcgtcc cccctgagga
     2521 tgggcagaca atcttcgatc gcatcgcgac ttccgataag cgcctggtat ggctggcgcg
     2581 cggcggtcac atcgccaccg aggattacga caaagagatc atatttgaag aggcgctgca
     2641 tttcatcgaa gcgcacacgc ccgcgaagcg ggcagtccaa cttccaacct ctaacctcca
     2701 acctccaact tccaacctcc aacatcatga cacgcgataa caagatcgtg acgatctcgt
     2761 tgttcctatg gggcatcggc gagagcatgt ggttttacat tcgcggcctg tatctcgaag
     2821 gattgggggc aatgccgcag gaagtggggt tggcgctggg gatcgccgcg ctgacacacg
     2881 ccgcagcata cgtgttgggt ggtgtgctcg ccgatcgctt cgatcgaaag ttcctgctgc
     2941 tggccggctg gctcaccggc gtggtcgctc cgccgctcat ggcgctcgcc ggcgactggc
     3001 gcgcgttcat gatcggcttt agcgtgtaca acgtatccgg tttcgtcgtc cccgtcatcg
     3061 acacctacgt cacgcaggcc gccggccgcg cgccgctgga gcgcgtgctg ccggtcgtat
     3121 tcgccggtta ctgggtgggc agcgtgctcg gtccgcaggt tggcaaggcg ttattgacga
     3181 tgatggacga gcgcggcatc ttgctggtct cctcgctcgt gttcgtcgcg tcgacggtgg
     3241 tgattgcgtt cgtccacgtc cagcctgcgg cgcggctgac cctctctggg gctggcttgc
     3301 ggcattcgcg tgtggggccg gttcaagcgc tgcgccctgc cctgtcgttc ttcgtctacc
     3361 tgttcgccat cttcttcagc atggcgctcg gcgcgcagtt gatcccgaac tacctgctcc
     3421 gccttggctg gcggtcgatt gacgtcagcg ggatcgtcag cgcgctgccg ctcggcatcg
     3481 ccgtcctgac cgtcgccgtc ggccacttcg cagcaggtcg caaccggcgc ggactgctgg
     3541 cgtcccaggc attagtgctg gccggaatcg ccgcgttcgc attcggcgcg ccggcctggg
     3601 gagggtttgc cgtggcaggg tacttgctgt tgggcgggtt cgatgcggcg cggcagcagg
     3661 cgctggcccg gatgccgcgc tacgtgcggc aggagcagcg tggcctggct ttcggcatgg
     3721 cgtcgaccgt gggcgaaggc gcgatcgcgg cggcttcggt gatcggcggc gtattgttcg
     3781 cgctcgatcc ggcgctgccc ttctacgccg gcctgatgct gatcccggcc ggcatggcgc
     3841 tgacgctgcg cctgcgccgc ccgcagaccg ccgtggccgc cgatgtgacg gcggcgcatg
     3901 ctggggcaac agttgcactg tcgcccggcg tccgcagtcc aactgcggac gaacggactt
     3961 gtgggtgacc accggaaagc ggggaacgat ttgagcatcc gcgagaacct gatgtgggaa
     4021 ggccgcacgc tcacggcgac atggctgcct gcgccgttca tgcctgcgct cgaccaggtg
     4081 acgcaggcta gcggcgtgtg cttcaccggc agcggcaaga tcgtcctggt ggcaggtcac
     4141 accggcaagt gggcgctgcc cggcgggcat gtcgagccag gcgagacgct ggagcagacg
     4201 ctggcgcgcg aagtgctgga ggaagcttgc gcgatagtca cgcagtccga gtatctcggc
     4261 gcgcaggagg tgagcgatcc ggacaatccg accggcccgc gcgtctacta tcaggcgcga
     4321 ttctgggcgc gcgtcgagct gaagcggttc gagccacggt tcgagcggat gcagcgcacg
     4381 ctgatcgcgc cgtccgcgtt tcagtcgacg ctgcgctggc gcactgaacg tatcgcgcaa
     4441 gcgatcctgg acgccgcgct cgcggcggaa acccgttacc ggtcgcgccc gcgcaagagc
     4501 gcgtcttaaa acccgtggag cgggcatccc tatacccgct gttgatgcat ggggatgcgt
     4561 caaccacggg ttggggatgc gtcaacacag ggttttaaga cagtctccaa gagggtgaag
     4621 gaggaaatcg gcgatgaggt ggacgcggcg tgatttcctg cgcgcgctcg ccgtgacact
     4681 aggggcggcg gcgcctcccc ctgacggcta caagatctac ttgcctctcg tgacgagaag
     4741 cagcgcgtac gccgccgatc tgggcggcgg cgtatgggtg atcggcaacg atctcgtcga
     4801 gcagcgcatc gccttcatcg gcggtgggct gttcacaagg tcgtttctcc ataaggctag
     4861 cgggcgggat tggtgcgctt cggcgtcgtc gccggtgttc ttccttgagg aagaggaggc
     4921 agagctgact tccgtggacg gctgggtgca catcgagtca gcggcgctca acttcggcga
     4981 cggcgcaaaa ctgacggtaa ccatgcagca cgcgtcacgc ccgatccgcg cgcatgccca
     5041 ctttcacgta tggtccgacg cgccggttgt gcgctcctgc accgaatacg agaatatcgg
     5101 cgccggcgcg cgccagttgc aggcgcagtc cttctgcgcc ctgacgctgg gcgcggctgg
     5161 cgcgctcgaa gtcgtatggg tcgcgccgtt tagctggctc tgggatcgca cggacgattc
     5221 atttgtcacg caccgcgaga cactccagaa ccccggccag gagcggcatc tagtgatggg
     5281 gccgtacgcc gcagaggatg tgggcggtta caacgtcgtc acctatcggc cgtcgtgtgg
     5341 atggttcgcg tttcatcaga agaccctcgg cgcgggcctg ttcggcggcg tcgagtggag
     5401 cggcgcgtgc gaagctcacc tatcgctgaa tgactccggc caggggcgga ttggcatcgg
     5461 gcactggcgg ccggccttta cgcacgcagc cgggccgggc gagcgtgtga cgtcgccagt
     5521 cgcgttcgtc ggcccgttcg agggcgcgcc cgacgaggcc gcccatctca ctcaccggct
     5581 ggcaaaggcc cactatatcc ccgctcggcc ttcgatccgc gtgcctgccg gggcagagtt
     5641 cccctacgtg atggccgaca cgtggggcta cggcccgaac atcgacgagg ccaggctggg
     5701 cgtgttcgtc gatcgggcgg ccgagatcgg cgtcgaggcg ttgacgatcg acgaaggctg
     5761 ggaggcgcgc atcggcgact gggtgtcgca cccggcgcgc tttccgagcg gcttgcgtgc
     5821 catggtcgac accatacaca gtaagagtat ggctgccgga ttgtggtttg ccttcgccaa
     5881 cgtcgatccg gactcggcgg ttgcgcgcgc gcatctcgac tggctggcga cgcggggcgg
     5941 ctcgcccatc ggggggtcgt tcaacacaca agtgttatgc ctgagccatc ctgacgcgcg
     6001 ggcgtgggtg gctgccgagt tcgaccgcat cgtcgccgag ttcgacgtgg acgccctcgt
     6061 gcaggacttc gagaccatcg cgcgctgcga caacccggcg cacgcggcgt ggcatcaggc
     6121 ggtggactcc gaatatcgca acgtcctggc attatgggag ctcatcgacg gcgtgcgggc
     6181 gcggcatccg aacgtaatga tcgagaacaa ctggagcggc gggcgcgtga tggacttcgg
     6241 catgctgcgc cgatacgaca cgtcggtgtg cgacgactac aaccaggcgg cgcgcaatcg
     6301 cggcgcgtcg ttcggcgtaa cgcacttctt cccgcccacc ttcgtctcca aatacatggg
     6361 cgcggaggcg ctgccgttcg attaccagac gcgcagctac ttcttcggcg ggccgtggaa
     6421 tctgatgacc gatttgcccg cgcaggacgc gcagatgctg gccggtgcaa tcggagcgta
     6481 caaggaactg agggaactga tccgcgacgg gcgcgtggtt cacctcctcg cgccgtcgtt
     6541 cagcggtacc gggccaaatc gttatcagat ttcgtgggac gcgattcagg cggtcgagcc
     6601 ggggaggaat cgagccgttt tgctgatcgg acgcggcctg gccgggccgg ggacgtacaa
     6661 cgtcaagccg caagggctga atccgacgaa agtgtatgcg ctcgccgcgt cgtcgggcgc
     6721 gaattatggc agccgctcag gcgcagacct gatggcgaat gggatcgacg tgagcctgcc
     6781 ggagcggacg cacgagacga tcgtcctcgc ataacgcagc ctctggccgc aaccaaaaca
     6841 ctaaccacgg atggcacgga ttacacggac attgagaggc cgctttggga cagtgaagac
     6901 ttccgaagac acatgcttgt cacttgagaa gagcgattgt gctcggcgac cactggtgct
     6961 atactttgtt tgcagtcagg gatgttcact taaagccgac aacgctctcc tcagttctcc
     7021 gcagcacaac gtatacaatt ctgaccatcg ttcagaatcc ctgcaaggag gcatgcgcat
     7081 gagcacccgg caccttcgca ccaggctttc agtcgtgatc ggcataacgg gactgtggct
     7141 cgtggcggtc ttgatgttca ccacggcgca atggggcagc gccgacgcgc ac
//
LOCUS       DBNHXB010000095         9825 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_688980, whole genome shotgun sequence.
ACCESSION   DBNHXB010000095 DBNHXB010000000
VERSION     DBNHXB010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9825)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9825)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9825
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_688980"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="AC162G_03675"
     CDS             <1..681
                     /locus_tag="AC162G_03675"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5074947.1"
                     /translation="YGSRLVVDVERLEVRHGETLAIVGPSGSGKSTLLRLMQFLERPS
                     SGRIVFDGRAIEAPAPLDARRRVTTVFQHPLMFDRSVRDNIAYGLRLRGRNNARAVVD
                     RLIDELGLRDLAEEPARLLSGGEAQRVALGRALAVEPDALLLDEPTANLDPYNVGLIE
                     NMIREQQRRGATIVMVTHQVFQARRLADRTGLLLDGRLIELAPTVQFLDSPHDPRTRA
                     FLSGEMIF"
     gene            875..2176
                     /locus_tag="AC162G_03680"
     CDS             875..2176
                     /locus_tag="AC162G_03680"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR5074948.1"
                     /translation="MADQAIAQPIRGVASPTLHRVILFSERLGRLTWPGQTAIVVAYT
                     AIVALVWGFALASFAAGFPAALVGFVFMAIDWIGLALLPKRRRSFGPVAPGLVLFGGA
                     RCTLSVTLAVFAREPNIAWSLLLCGHLALTGYAMDSMWGEPFRVGVTRRTYRSPKLDG
                     APPLRIAHLSDFHVERLTQREAKVLALLDEMRPDLIVYTGDLLSYSYIDDPVAQADCR
                     ALMSRLHAPLGVFAVTGTPLVDTETALQNVLGDLGNITLLRDCVASLPQCPGIKLIGL
                     TCTHNPALDAPNLDSVVDGLAPSDYTMLLYHAPDLMPEAARLGIDLVLCGHTHGGQIR
                     LPVFGAVFTSSIYGKRYEMGEYREGSTTMYVSRGIGMEGKGMPRMRFLCEPEIELIEL
                     RGTLEAEEALGDTTPVSHRARPPGSRSLYAPRATSRMKAPP"
     gene            2173..2538
                     /locus_tag="AC162G_03685"
     CDS             2173..2538
                     /locus_tag="AC162G_03685"
                     /inference="COORDINATES: protein motif:HMM:NF016001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HIR5074949.1"
                     /translation="MKPSRPDEHLLVDASELVLVSAGDPGDPPHEARLRFAYDDGAVY
                     LLAHTDADWYRNIARDPGVVVRIRQRGFRGRARLPDARQRAHTIDQIGTLFRRKYGAG
                     ALKGIDPNALLPVIVDIQF"
     gene            complement(2653..3774)
                     /locus_tag="AC162G_03690"
     CDS             complement(2653..3774)
                     /locus_tag="AC162G_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE/TadZ family protein"
                     /protein_id="HIR5074950.1"
                     /translation="MNERILIIDDDPDALTLIGLTLERRGYSVLKAAGGSEALMMIES
                     DRPDLVLLDLMMPHMDGYEVCRRIKADPRLTGIPIVMLTAKAQMASQVEGYRVGADDY
                     VTKPVHPDDLASHIHAVLERAKEWQPKKTGGRVVGVIGVKGGVGTTTVAVNVAVTLAA
                     TRRTILADFDAGGTAAIHLGFSALPEPEGLASLPASAIDQDSVAKSLVAHDSGLHFLP
                     PANGALVPEQAAAIFSHLLDLCEVCVLDLGGGLSVVSRALLERCDLVLIALDSDRAAV
                     LQAQRLLLALTELGVNRSRLRMVWANRSGLDEQAALGTLRAAVGEAPIHVVDPAAELA
                     HRSVESGQPLVLSDPDSAAAIELRELGQSVLAALEGVAE"
     gene            complement(3865..4065)
                     /locus_tag="AC162G_03695"
     CDS             complement(3865..4065)
                     /locus_tag="AC162G_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074951.1"
                     /translation="MPGILRANGCPHMRLEGRVARGFLGFGRRVVVTASCERSGGSVA
                     EPYVGCGQCHLDAPSASLLGEE"
     gene            complement(4325..4882)
                     /gene="efp"
                     /locus_tag="AC162G_03700"
     CDS             complement(4325..4882)
                     /gene="efp"
                     /locus_tag="AC162G_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR5074952.1"
                     /translation="MIDVNDLRKGTTFALDGELYRVLEYSHIKPGRGNATIRTKIRNL
                     RTGTTMEKTFQSGDRVQDIRIETHEMQYLYHDGDLYHFMDTATFDQIAMAEATLGDAV
                     HYMTDNMMIMVQEYNGEPLGVELPTAVDLKVTEAQAAIRGDTATGATKVVTVETGLRV
                     PVPMFVKAGDRIRIDTRTGEYVTRV"
     gene            complement(4955..6979)
                     /locus_tag="AC162G_03705"
     CDS             complement(4955..6979)
                     /locus_tag="AC162G_03705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR5074953.1"
                     /translation="MPIARADVIVTSMLAASRRWTLLPSRFDPAWRMVILITAAVLLA
                     YGDSITLPFHFDDIPHYLWLNDQSIGSIWLSAAGRPYYRPMQFFGWKLYETAFGRDSV
                     VVYHLMSLLVHALDAVLVALVARRLVDAKRGEGWWRATTAGLTFALFPFSYQVVPLPS
                     SFTHPMVVLFVLLSILAYDRFRMRGRRAWRWLIVALVCGVFAFASNEGSLVLAAAIAL
                     YEAFGRAPRPIRWRWAGLFVLLAAVYFAWYQSRPLQAGGEVRVRGLEAIVQNAMVVLQ
                     GGTFPPQPIGRALMDEGFTDHSAVLSIGFLALSTLAVMYVRAGEMRGLVFGAGWFVIF
                     MVVPALLLSHDYLINAPRVLYLSACGAAWLWAGAAGVVRRQGRRLVALAFVAAVLAPS
                     FIFIRQRMALYHLNAAPLQEAIDVALRAGPADRLLLVNLPAWVSAERQWYPIGHEGVL
                     FLPAYATLSDFVSVNTGQPSQAIGVEFNNLTTPQAYHYGVYGPPLDYERLALEERSAL
                     EVHLAAYRPDVIDLNAAGRVLDQTRPAGADRASFDSLVALESATWLACADRLGVTLDW
                     RADAFPPGDAHIFVHVLDARGVLIAQHDSPPLQGLLPFWQWQPGDRARDMHPIDLPAP
                     LEDGAYTIAVGLYDVVTGQRVPAALVDGSRPPNDAVPIGEFMPGQQAVCP"
     gene            complement(7014..7841)
                     /locus_tag="AC162G_03710"
     CDS             complement(7014..7841)
                     /locus_tag="AC162G_03710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5074954.1"
                     /translation="MPPKVIVIMPAYNAARTLERTYHDLPHDVIDHVILVDDVSQDET
                     VEIARRLGLHVVVHIQNKGYGGNQKTCYVEALRAGADVVVMLHPDYQYDSRLVPDLIK
                     PIVEDRADMMLGSRLLTSGSARVGGMPLWKYVANRFLTIVENLVLGVRLSEAHTGFRA
                     YRRKLLETIPFLLNSDNFVFDTQVIAQAVAFGFRVGEIAVPTRYFSEASSVNFRRSVV
                     YGLSTLVVMLRFALNRIGVLRADEFRLTLRQVISRYHAAEIIDDAGAPPHSPERPPS"
     gene            complement(7861..>9825)
                     /locus_tag="AC162G_03715"
     CDS             complement(7861..>9825)
                     /locus_tag="AC162G_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074955.1"
                     /translation="PAPLVPRWPLYAGVALAIGLTSLLYLNTLSLPFFQDDVIHIRWL
                     STRSLADPFLTAENLPTYRPLGESLMKLWWSVLGRHDPVTLRLQNILTHALNATLVAL
                     LALRLDRSARRYVVGGVAAALFAAFPFAYQAVVWINVFFYPLGTLLLLLAALSYWQAR
                     TAGSIRWLLVAWLMCFLAPAEIEWGLMSGALLVGIELVLWAQRRQARPWLIGPAIGLA
                     INAVFFAIWQIVPKFDYSGGMRINLEAMLQMSTYYLQGLIYPSAPLAVPLVGLAGLDD
                     LNAVRWVALATLMVVLSLLIWRRRLVLAMAALGWFALLVFPTLVTVDFDYVINSPRLL
                     YPAAVGIVILWAAWIAEFTAPGRWRPARMVIAAGAFGLALAQNVAFVQLQNRLYHLAE
                     APVHALAAAARAAEDGEVPLFVNLPSWLAPHWRDYAIGNHGVQFITGYAGIDDVIFAY
                     NGADRFSRAVSFTNVMSITPYWLGLYGPKLDWQDLARVMEQSDPVFLTTYDGDGIRLR
                     PAGRVKGVPQARAAARFGDTPPSVELGAALVTGQDKSIDVELNWRVLSSIDRDLSVFV
                     HLYAPDGRLVAQADGYPLLGLSPFWLWEPGQTLLDMRSLAWPAGASPGEYRIGVGVYD
                     RASGERLAAFEPDGSRLPDGTFLVATLGRP"
BASE COUNT         1886 a         3228 c         3133 g         1578 t
ORIGIN      
        1 tacgggtctc gtctcgtcgt cgacgtggag cggctggagg tgcgccacgg cgagactttg
       61 gcgatcgtcg ggccgagcgg gtcgggcaag agcactctgc tgcgactcat gcagtttctc
      121 gagcgcccca gctccgggcg catcgtgttc gacggccgtg ccatcgaggc tccggcgccg
      181 ctcgacgcgc gccgccgtgt aacgactgtt ttccagcatc ccctgatgtt cgaccgatcg
      241 gtgcgcgaca acatcgccta cggcctgcgc ctgcgcgggc ggaacaatgc gcgcgccgtc
      301 gtcgaccgcc tgatcgacga gctggggttg cgcgatctgg cggaagagcc ggctcgcctg
      361 ctctccggcg gcgaggccca gcgtgtggcg ctcggccgcg cgcttgccgt cgagcctgac
      421 gcactgctgc tcgacgagcc gaccgccaat ctcgatccgt acaacgtggg cttgatcgag
      481 aacatgattc gcgagcagca gcgccgaggc gcgacgatcg tcatggtgac acatcaggtg
      541 tttcaggcgc gccgcctggc cgatcgcacc gggctgctgc tagacgggcg cttgatcgag
      601 ctggcgccca ctgtgcaatt cctcgactcg ccgcacgatc cgcgcacgcg cgcattcctc
      661 agcggcgaga tgatcttcta gggcaaccag acttccgaag tctcctctaa cttcttctga
      721 cacgtgcgca acgcatctgt aacgcaacgt cgcacggctg aaacgttttc tgccttgatt
      781 cgtcccgtcg cacgcggtat gatgggtgag acttgagtac cagccaccct ctgtgacgcc
      841 ggtggcctgg acggcgacga gatacccgca caccatggcc gatcaagcta ttgcgcaacc
      901 gatcagaggc gtggcctcgc cgacgctgca tcgcgtgatt ctgttttccg agcgactggg
      961 gcggctgacc tggcccgggc aaactgccat cgtcgtcgcc tacacggcaa tcgtcgctct
     1021 ggtttggggc ttcgcgctcg cttcattcgc cgccgggttc cccgccgcgc tggtcggctt
     1081 cgtattcatg gcgatcgatt ggatcgggct ggccctcctg ccgaagcgga ggcgctcgtt
     1141 cgggccggtc gcgccgggac tggtactgtt cggcggggcg cgctgcacgc tgagcgtcac
     1201 gctggcggtc ttcgcacgcg agccgaacat cgcatggagc ctgctgctgt gcggacatct
     1261 ggcgctgacc ggctatgcaa tggatagcat gtggggcgag ccgtttcgcg tgggcgtcac
     1321 gcgccgcacg taccgatcgc ccaagctcga cggcgcgccg ccgctgcgca tcgcgcatct
     1381 ttccgacttt cacgtcgagc ggctcacgca gcgcgaagcg aaagtgctgg ctctgctcga
     1441 cgagatgcgg ccggacctga tcgtctacac cggcgatctt ctttcgtatt cttacatcga
     1501 cgaccccgtc gcgcaggccg attgccgcgc gctgatgtcc cggctgcacg caccgctggg
     1561 agtgttcgcc gtgacgggca cgccgctcgt cgataccgag actgcactcc aaaacgtgct
     1621 tggcgacttg ggcaacatca ccctgctccg cgactgcgtc gcgtcgcttc cacagtgtcc
     1681 gggcatcaag ctgatcggcc tgacctgcac gcacaaccct gcgctcgacg cgcccaacct
     1741 cgattctgta gttgacggac tggcgcccag cgattacact atgttgttgt accacgcgcc
     1801 cgatctgatg cccgaggcgg cgcgtctggg catcgatctc gtgctgtgcg ggcacacgca
     1861 cggaggccaa atccgcctgc ccgtcttcgg ggcggtgttc accagctcga tctacgggaa
     1921 gcgatacgag atgggcgagt atcgagaagg gagcacgacg atgtatgttt cgcgcggcat
     1981 cggcatggaa ggcaaaggca tgccgcgcat gcgcttccta tgcgagccgg agatcgagct
     2041 gatcgagctg cgaggcacgc tggaggccga agaggcgctg ggcgacacga cgccggtgag
     2101 ccatcgcgcg cggccgccgg gtagcagatc gttgtatgcg ccgagggcga cctcccggat
     2161 gaaggcccca ccatgaagcc gtcacgaccg gacgaacatc tgctcgtcga tgcgagcgaa
     2221 ctggtcctcg tcagcgcagg cgatcccggc gacccgccac atgaagccag actgcgcttc
     2281 gcgtacgacg atggagcggt ttatctgctg gcgcacacgg atgcggactg gtatcgcaac
     2341 atcgcgagag accccggcgt cgtggtgcgc attcggcagc gaggcttccg aggccgcgcg
     2401 cggctgcccg acgcccggca gcgcgcacac acgatcgacc agattggcac gttattcagg
     2461 cgaaaatacg gagcaggcgc tctcaaggga atcgatccaa acgcactgtt gccggtgatc
     2521 gtcgacatcc agttttagtg acaacacgcc cctgaccggg gcgcgttttg tcgttataag
     2581 acctgccagg tctcggagac ccggcaggtc tgtagaagtt cgggcggatg ccgggaggtc
     2641 ggtcgcacag cgttattccg cgacaccttc cagcgctgca aggactgact ggcccagctc
     2701 gcgcagctcg atggccgccg ccgagtccgg atcgctcagg actagcggct gtcccgactc
     2761 gaccgaccga tgagccaatt ccgcagccgg atcgacgacg tgaatcggcg cctctccgac
     2821 tgcagcccga agggtgccga gagcagcctg ctcgtccagg cccgaccggt tggcccacac
     2881 catgcgcagc cgcgagcgat tcacgcccag ctccgtgagc gccagcagca agcgctgcgc
     2941 ttgaagcacg gccgcgcgat ctgagtcgag cgctatcagc accagatcgc agcgctccag
     3001 cagtgcgcgc gaaacgacgc tcagaccccc gcccaggtcc agcacgcaaa cctcgcacaa
     3061 atcgagcagg tgtgaaaaga tcgcggccgc ctgctcgggc acgagcgcgc cattcgcagg
     3121 cggcaggaag tgcaggccgc tgtcatgcgc gacaagcgat ttcgcgacgc tgtcctggtc
     3181 gatcgcgctt gccggaagcg aggccagtcc ttcgggttcg ggaagcgcgc tgaatccaag
     3241 atgaatagcc gccgtgccgc ctgcgtcgaa gtcggccaga atggtgcggc gcgtggcggc
     3301 cagcgtgacg gccacattga cggccaccgt cgtcgtgccg acgccgccct tgacgccgat
     3361 cacgccgacg acgcgtccac ccgtcttctt cggctgccat tctttcgccc gctcgaggac
     3421 tgcgtggatg tgtgacgcca ggtcgtccgg atgcaccggc ttggtcacgt agtcatccgc
     3481 gccgacgcga tagccttcca cctggctggc catctgggct ttcgccgtga gcatcacgat
     3541 gggaatgccg gtcaggcgcg ggtcagcctt gatgcgacgg cagacctcgt aaccatccat
     3601 gtgcggcatc atcagatcga gcagcacgag gtcggggcga tcggactcga tcatcatcag
     3661 cgcctccgag ccgccggccg ccttgagcac cgaatagccc cggcgctcaa gcgtcagccc
     3721 gatcagggtc aaagcatcgg gatcgtcgtc gatgatgagg atgcgttcgt tcacagttgc
     3781 ctcccatgcc gggcgttgcg cgctcgcgcc ggcacctcga cagcctgccc caagtttagc
     3841 ccattgcctc gaaaatgcaa tagcttattc ctctcccagg agcgatgcgc tcggcgcatc
     3901 gaggtgacac tggccgcacc cgacgtacgg ctccgctacg ctgcctccac tgcgctcgca
     3961 cgacgccgtg acgacgaccc ggcgcccgaa acccaggaac ccgcgggcga cgcgtccttc
     4021 cagtctcatg tgcgggcagc cgttcgcacg cagaatgccg ggcacggggc aatcgcggca
     4081 caaatcaggg gaccaatcgc gcgacgaaga ggattgttca accagacggc attcctggag
     4141 ggagcggcca cgatggaagt cttcatagta gtaacgacac tcttgaccga atcgagtacg
     4201 catagcgcga ttatacctgt aacctcacga cgagccaacc gattgaagcg cagtaacaaa
     4261 cgaaaagccc acccagagac tggatgggcc atcctgtgga ccgccacgtc aaaaggcgcc
     4321 cgcctcacac ccgggtaacg tactcgcccg tgcgcgtgtc gatacgaatc ctgtcgcccg
     4381 ccttgacgaa catcggcacc ggtacccgga ggcctgtctc gacggttacg accttggtcg
     4441 cgccggtcgc cgtgtcgccc cggatggcag cctgggcctc ggtcaccttg agatcgaccg
     4501 ccgtcggcag ctcgacgccc agcggctcgc cgttgtattc ctgcaccatg atcatcatgt
     4561 tgtcggtcat gtagtggacg gcgtcgccca gcgtcgcctc ggccatcgcg atctgatcga
     4621 acgtcgcggt gtccatgaag tgatacaggt cgccgtcgtg atacaggtac tgcatctcgt
     4681 gcgtttcgat gcggatatct tgaacccggt cgccggactg gaacgtcttt tccatcgtcg
     4741 tcccggtgcg caggttgcgg atcttcgtgc ggatcgtcgc gttgccacgg ccgggcttga
     4801 tgtgcgagta ctccagaact cgatacagct cgccgtccaa cgcgaacgtc gtgcctttgc
     4861 gcagatcgtt gacgtcaatc atagtaaacc gactgctccc acttatgaaa tgcctaacga
     4921 gattataccg ggagccggat ggctgtcaac cctcctacgg gcatacggcc tgctggccgg
     4981 gcataaactc gccgatcggc acggcgtcgt ttggcggccg cgagccatcg accagagcgg
     5041 ctgggacgcg ctgtcctgtc acaacatcat aaagcccgac ggctatggtg tacgctccgt
     5101 cttccaaagg cgccggcagg tcgatcgggt gcatatcccg ggcgcggtcg ccgggctgcc
     5161 attgccagaa ggggagcagc ccctggagcg gcggactgtc gtgctgcgcg atcagcacgc
     5221 cgcgcgcgtc gaggacgtgg acaaagatat gcgcatctcc cggagggaac gcgtcggcgc
     5281 gccaatccaa tgtaacgccc agccgatccg cgcatgccaa ccacgtcgcc gattccaacg
     5341 cgaccaggct atcgaacgaa gcgcgatccg ctccggccgg ccgggtttga tcgagaacgc
     5401 gcccggctgc gttcagatcg atcacgtccg gcctatacgc ggcaaggtgc acctcgagag
     5461 cagagcgctc ctcgagcgcc aggcgctcat agtccagcgg cggtccatat acgccgtaat
     5521 ggtaagcctg cggcgtcgtg aggttgttaa actcgacgcc gatcgcctgc gagggctgcc
     5581 cggtgttgac ggacacgaag tccgacaacg tcgcgtacgc gggtaaaaac aacacgccct
     5641 cgtgaccgat cggataccat tgccgctcgg cgctgaccca cgccggcaaa tttacgagca
     5701 gcaggcgatc cgccgggccg gcgcgcagcg ccacgtcgat ggcttcttga agcggcgcgg
     5761 cgtttaggtg gtaaagcgcc atgcgctggc gaatgaagat gaacgagggc gcaagcacag
     5821 ccgcgacgaa agctagagcg accagccgcc gaccctggcg ccggacaacg cccgctgcgc
     5881 ctgcccacag ccacgctgcg ccgcacgccg ataaatagag caccctcggc gcattgatca
     5941 ggtaatcgtg ggacagcagc aacgccggca caaccatgaa gatcacgaac cagcccgcgc
     6001 cgaagaccaa tccgcgcatc tcgcccgctc gcacgtacat tacggccagc gtcgacaggg
     6061 cgagaaagcc gatcgaaagc acggcgctat gatcggtgaa gccctcgtcc atcaacgcgc
     6121 gcccgatcgg ctgcggcggg aaagtccctc cctgcagcac gaccatcgcg ttctgaacga
     6181 tggcctccag gccgcgcacg cgcacttcgc cgccggcctg cagcggccgg gactggtacc
     6241 aggcgaagta gacggcggcc agcaacacga acagtccggc ccagcgccaa cgaatagggc
     6301 gcggcgcgcg gccgaaagcc tcgtagagcg cgatggccgc cgcgagaacc aggctgccct
     6361 cgttactggc aaacgcgaag acgccacaca ccagggccac gatcagccag cgccacgcgc
     6421 ggcggccgcg catccgaaat cggtcgtagg ccaggatcga cagcaagacg aatagcacga
     6481 ccatggggtg cgtaaaggag gagggcaagg gcacgacctg atacgaaaat ggaaacagcg
     6541 cgaaggtcag accggcggtt gtcgcgcgcc accatccctc gccgcgcttc gcgtcgacga
     6601 gccgccgcgc cacgagcgcg accagaaccg cgtcgagcgc atgaaccagc agactcatca
     6661 ggtgatagac gacgacagaa tcgcgtccaa aggccgtctc gtatagtttc cagccgaaga
     6721 actgcatggg ccggtagtag gggcgacctg ctgcggaaag ccagatcgag ccgatggact
     6781 gatcgttgag ccacaaatag tgggggatgt cgtcgaagtg aaacggcagc gtgatcgaat
     6841 cgccgtatgc cagcaacacg gcagcggtga tcaggatcac cattcgccag gccggatcga
     6901 atctgctcgg caggagcgtc catcggcgtg acgcagcaag catcgaggtc actatgacgt
     6961 ccgctcgggc gatcggcaca cgctccacgc tccacgcagc atgtgtatcg gggtcacgat
     7021 ggcggccgct cgggcgaatg gggcggcgcg cccgcgtcgt cgatgatctc agccgcgtga
     7081 tagcggctga tcacctggcg cagcgtcagg cggaactcgt cggctctgag cacgccgatg
     7141 cggttgagcg cgaaacgcag catcacgacc agcgtcgata acccgtagac gacgctgcgc
     7201 cggaagttga ccgaggatgc ctcagaaaag tagcgcgtcg gaacggcgat ctcgccgacg
     7261 cgaaacccga aggcgacggc ctgggcgatc acctgcgtgt cgaagacgaa gttgtccgag
     7321 ttgagcagga acggaatggt ctccagcaac ttgcggcgat aggcgcggaa gccggtgtgc
     7381 gcttcggaca gcctcactcc caggacgagg ttctcgacga tcgtgaggaa ccgattggcg
     7441 acgtacttcc acagcggcat gccgccgacg cgcgcgctgc ccgacgtcag caggcgactg
     7501 ccgagcatca tgtcggcccg atcttcgacg atcggcttga tcaggtcggg cacgagccga
     7561 gaatcgtact gatagtcggg atgcagcatc acgacgacgt cggcgccggc tcgcagcgct
     7621 tcgacgtagc acgtcttctg gttgccgccg tagcctttgt tctggatgtg cacgaccacg
     7681 tgcaagccca agcgccgcgc gatctcgact gtctcgtcct ggctcacgtc gtcgaccagg
     7741 atgacgtgat cgatgacgtc gtgcggcaga tcgtgatagg tgcgttccag cgtgcgggcc
     7801 gcattgtagg caggcatgat cacgatgact ttcgggggca tcctgcttct cctgctccgc
     7861 tcatggccga cccagcgttg cgacaaggaa agtcccgtct ggcaggcgcg agccatccgg
     7921 ctcaaacgcg gccagacgct cgcccgaggc ccgatcgtac acgccgacgc cgatgcggta
     7981 ctcgccgggg gacgcgccgg ccggccatgc caggctgcgc atgtccagca gcgtttgtcc
     8041 gggttcccac aaccagaatg gcgacagccc caacaggggg tagccgtcgg cctgcgcgac
     8101 gagccgccca tccggcgcgt aaagatggac gaacaccgac aggtcgcgat cgatgctgct
     8161 cagcacgcgc cagttcaatt cgacgtcgat cgatttgtct tggcccgtga cgagcgccgc
     8221 gcccagctcg acactcggcg gcgtgtcgcc gaaccgggcg gccgctctgg cctgagggac
     8281 tcccttcacg cggcccgctg gcctcagccg aatgccgtcg ccgtcatacg tcgtgaggaa
     8341 caccggatcg gattgctcca tgacgcgggc gaggtcctgc cagtcgagct taggcccgta
     8401 caggcccaac cagtaggggg tgatactcat cacatttgtg aagctcacgg cgcgggaaaa
     8461 ccgatcggcg ccattatacg cgaagatcac gtcgtcgatg ccggcgtagc ccgtgatgaa
     8521 ctgcacgccg tgattgccga tcgcgtaatc gcgccagtgc ggcgcaagcc aagacggcag
     8581 gttgacgaag aggggcacct cgccatcctc cgcggcgcgc gccgccgccg ccaacgcatg
     8641 gacaggcgcc tcggcaagat gatacagccg gttctgcaat tggacgaacg cgacgttttg
     8701 agccagcgcc aggccgaagg cgcctgcggc gatgaccatt cgagcaggtc gccagcgtcc
     8761 cggcgcggtg aactcggcaa tccacgccgc ccacaggatg acgataccca ccgccgccgg
     8821 gtacagcagg cgcggagagt tgatgacgta atcgaaatcg acggtgacga gcgtcgggaa
     8881 tacaagcagc gcgaaccagc ccagggccgc catggcgagg accaggcgcc gccgccaaat
     8941 caggaggctg agaacaacca tcagagtcgc cagcgcaacc catctgactg cattcagatc
     9001 gtcgagtccg gccagaccca caagcggcac ggccagcggc gcgctcggat agatcagccc
     9061 ttgcagatag tacgtgctca tctggagcat ggcttcgagg ttgatgcgca tgccgccgct
     9121 gtagtcgaac ttagggacga tctgccagat ggcgaagaag acggcgttga tcgccaggcc
     9181 gatggccgga ccgatcagcc acggccgcgc ctggcgccgc tgcgcccaca gcacaagctc
     9241 gatgccgacc agaagcgcgc cgctcatcag gccccactcg atctcggctg gcgcgaggaa
     9301 acacatcagc caggccacaa gcagccagcg aatcgatccc gcagtgcgag cctgccagta
     9361 cgataatgcg gcgagcagca ggagcaatgt gcccagcgga tagaagaaca cgttgatcca
     9421 aacgacagcc tggtacgcaa agggaaacgc tgcgaacaac gctgccgcaa ccccgccgac
     9481 gacgtaccgc cgcgcggagc gatcgaggcg cagcgccagg agcgcgacca gtgtcgcgtt
     9541 caatgcgtgt gtcaggatgt tctgcaggcg caatgtgacc ggatcgtgcc tccccagcac
     9601 gctccaccac agcttcatca gcgactcgcc gagcggccga tacgtaggca ggttctcggc
     9661 agtcaggaag ggatcggcga ggctgcgcgt ggacagccac cggatgtgga tcacgtcatc
     9721 ttgaaagaac ggcaggctga gcgtattcag atacagcaac gacgtcaacc cgatggcgag
     9781 cgcaacgccg gcgtacagcg gccagcgcgg cacgagtggg gcggg
//
LOCUS       DBNHXB010000096         7322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1695136, whole genome shotgun sequence.
ACCESSION   DBNHXB010000096 DBNHXB010000000
VERSION     DBNHXB010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1695136"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="AC162G_03720"
     CDS             complement(<1..202)
                     /locus_tag="AC162G_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074956.1"
                     /translation="MFAIVVSSIGCGIAVGVGAVVGCAVAAETGVAVAAGCDLVSVTS
                     DRAQAWISGASIKLPPATAILRQ"
     gene            192..1091
                     /locus_tag="AC162G_03725"
     CDS             192..1091
                     /locus_tag="AC162G_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR5074957.1"
                     /translation="MANIALVKTGDRAEGMRRAIELLGLNPARGQRVLLKPNLNSADE
                     APGSTHPDILRALVVKLNDMGARAITVADRSGMGDTRTVMQRKGVFELAEELGLTAVV
                     FDELEERDWVIRQSKDMHWPRGYAVPRLLLDAECVVQTCNLKTHRFGGHFTLSLKNSV
                     GFAAKTVNGSGYDYMHELHSSPYQRQMIAEINTAYTPALIVMDGVEAFVDGGPDRGKK
                     VHAAVVLAGTDRVALDAVGVAILRLFGTTREVSQGRVFEQAQIARAAELGLGVDSPAR
                     IAIVTGDAESEMYAQQIREVLMR"
     gene            1223..1912
                     /locus_tag="AC162G_03730"
     CDS             1223..1912
                     /locus_tag="AC162G_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HIR5074958.1"
                     /translation="MPRRTSTGTCTLCREDFSKAAMTRHLIKCRAEHPPTRGKPRKTF
                     LLKVEGRYLPDYWLHIEIPAEATLRTLDAFLRDIWLECCGHLSAFTIENEQYELNTGG
                     VDAMWPMIFGRGAPPKSMNIRLEAVLRPGLKFFHEYDFGTTTHLALRVAAEQEGIVPR
                     NAVRILARNHPPPIPCEKCGAPATEVCAQCIYDGRGWVCASHGRTHKCGEDMLLPVVN
                     SPRVGMCGYTG"
     gene            complement(1920..2630)
                     /locus_tag="AC162G_03735"
     CDS             complement(1920..2630)
                     /locus_tag="AC162G_03735"
                     /inference="COORDINATES: protein motif:HMM:NF021255.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HIR5074959.1"
                     /translation="MKKHKKRPQIDRRLRRPAPRSTGGIQLVTPEGTPLVFASARYRH
                     ADPEGIRQVLGAAEDFDAPGPEEMGQAQQTFGWLQTKPIPGVTDPALGYRVLASLTLT
                     PTMLEIEAMSEQRLANCRRRLEELLGDRIRLEETSARSAAQALSERAPQAEPPGEPFV
                     PPPEVLAQIEEHLLRRWISEPIPALGGLTPLEAVKTPEGRRRVLELIDEGERMQQRYQ
                     GPGFAPDYRKTKKMLGLE"
     gene            complement(2839..2912)
                     /locus_tag="AC162G_03740"
     tRNA            complement(2839..2912)
                     /locus_tag="AC162G_03740"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2876..2878),aa:Val,seq:gac)
     gene            complement(3011..4156)
                     /locus_tag="AC162G_03745"
     CDS             complement(3011..4156)
                     /locus_tag="AC162G_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR5074960.1"
                     /translation="MGGSFKIATVRGIDIKIHVTFFLILIYAAIVFGSDERGMNGALF
                     GIVATLLLFACVVLHELGHSVVAQRYGIPVRDITLWPFGGVARLERIPDKPAQEFWVA
                     IAGPAVNVAIVAVIFVLAQVLARLQVGFDLGALERGMLQLEPASLLAYLFSTNLILAV
                     FNLIPAFPMDGGRILRALLATRINYSRATAIAVAIGQNLALLAGLFGFLTGQLNLVLI
                     AIFVFLGAGSEGQMVQVKSVLDGLKVRQAFTRKTEALNPDDRLARAVDLTLSSFQSDF
                     PVCRGEELVGLLTRTDLISALQDRGSNAFVVSVMRKEFPTVTLDDSVYVAQQRMSEHH
                     IEALPVMEQGQFRGLLTLRDIDEVFRLLSVSPQLLQSRKPIVEESVI"
     gene            4370..5356
                     /gene="holB"
                     /locus_tag="AC162G_03750"
     CDS             4370..5356
                     /gene="holB"
                     /locus_tag="AC162G_03750"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIR5074961.1"
                     /translation="MSDASHPTWPVVGHAWAVAQLARAIDEDRLSHAYLITGPAQIGK
                     ATLARAMAMAINCTGEARPCGACRPCVRIATSTHADVRVVAPEGERLKIDQIRELQRE
                     LSLSPVEARKRVAILDDFDRATIEAANALLKTLEEPASSVVLVVIAPEAELLLPTIVS
                     RCQVVALRPLTIAQVRDALVSRWGVDAGRADLLAHLSGGRIGWAITAAKDKSVLERRA
                     ARLDHLQRLFGASRVERFAYAELLARSADTREAIEVWRTWWRDVMLASANSGAELTNV
                     DRRDEIERVAARLDVQQARSAAEACGRALWQLDKNAVPRLVVEVMLMSLPKI"
     gene            5418..5885
                     /locus_tag="AC162G_03755"
     CDS             5418..5885
                     /locus_tag="AC162G_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5074962.1"
                     /translation="MTFDEWLASVPAEITDDPLWRMEVYRLALFAGDLAWHDVSRLAQ
                     DKRTISLSGQLYEAVGSISANIAEGYSRSSGKDEARFYEYSLGSAREARTWYYQGRHV
                     LGAVVAGHRIKLLTQIIRLLLTIIPAERRRKLSDEQVAYDTNIAYLLNNPPMP"
     gene            complement(5931..>7322)
                     /locus_tag="AC162G_03760"
     CDS             complement(5931..>7322)
                     /locus_tag="AC162G_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR5074963.1"
                     /translation="DWVRSNGDSLGQPVYSVGHGTVTYAQPLGWGVDRGVVIVRHLFP
                     DGGTILSFYGHLDPDSVELRPGDCVARGDQVGAIGKPRGRPHLHFEVRNHTPDDPGPG
                     YWPVDPRLAGWQPPSEYIWTYRIATAPGVQWTRPFTATASTGIGLLSDGALAALDGQR
                     LIVVDPNDGSLRWSRPVSGTILSSVVDASGSAIYLSRQGGAVEAVDASGETVWQAQFA
                     TRARPYLLPLPGSGVVVHNENRLLGIGTDAKRLWLIQNVAPPFDWTLDGDRLIFTTVA
                     QQPTLYALDRSGRLLAGASIGGRPVVSGDRMFLYNPNGIYQIDPGTLTARLLKPLDPS
                     VFEIGTIAALPDGGVVVSHRGEFARRLIALDADGSLRWDWAIAGLGTTMPRLLANGDR
                     VYAIAQDGDVLSIDLASGAARRVFDGGSAIGLEGSPWAAFTPDGRLLFDFRGGNIVAL
                     DPEAAMEVIVGAH"
BASE COUNT         1397 a         2340 c         2343 g         1242 t
ORIGIN      
        1 tctgccgaag gatggccgtg gccggcggga gcttgatgct tgcgccgctg atccaggcct
       61 gcgcccggtc agaagtgaca ctcaccagat cacagcccgc agcaactgcg acgcctgtct
      121 cggccgcgac agcgcaccca accactgccc caactccaac ggcgatgccg cagccaatag
      181 aggagacgac aatggcaaac attgccctgg tcaagaccgg tgatcgcgcc gagggcatgc
      241 gccgggcgat cgaactactt ggtctcaacc cggcgcgcgg ccagcgcgtg ctgctcaagc
      301 cgaatctcaa cagcgccgac gaagcacccg gctcgacaca ccctgatatt ctgcgcgcac
      361 tcgtcgtcaa gttgaacgat atgggcgcgc gggccatcac cgtggccgac cggagtggca
      421 tgggcgacac gcgcacggtg atgcagcgaa agggcgtgtt cgagctggcg gaagagttag
      481 ggctcaccgc tgtggtgttc gacgagctgg aagagaggga ctgggtcata cgccagtcga
      541 aagacatgca ttggccacgc ggctacgccg tgcccaggct gctgctcgac gccgagtgcg
      601 tggtgcagac ctgcaacctg aagacgcatc gcttcggtgg acactttacg ctgtcgctca
      661 agaactcggt cggcttcgcg gccaaaacgg tgaacggcag tggctacgac tacatgcacg
      721 agctgcacag ctcgccctat cagcgacaga tgattgccga gatcaacacg gcctacacgc
      781 cggcgctgat cgtgatggac ggcgtggagg ctttcgtgga cggcgggccg gaccggggca
      841 agaaggtgca tgccgcagtg gtgctggcgg gcacggatcg cgttgccctg gacgcggtgg
      901 gtgtggctat ccttcgcctg ttcggcacga cgcgcgaggt cagccagggt agggtatttg
      961 agcaggcgca gatcgcgcgg gcggcggagc tgggtctggg cgtggactcg ccggcgcgga
     1021 tcgcgatcgt cacgggcgac gcggagagtg aaatgtacgc gcagcagatc cgggaagtgt
     1081 tgatgcgatg acgatccgtt gagatcaata actcgtttac ttcaacaacg acatcggcgg
     1141 cgtcgcgctg caaaatgcga agacactgag aagattgctt gagagacggg agtgaaaagg
     1201 gatcgacaga aaggagattg ccatgcctcg ccgaacctcc accggaacgt gcacgctgtg
     1261 ccgcgaagac ttcagcaaag ccgccatgac ccgccacctg atcaagtgcc gggccgagca
     1321 tcctcccacc agaggcaagc cccgcaaaac cttcctcctc aaggtggaag ggcgctacct
     1381 gcccgactac tggctgcaca tcgaaatccc cgccgaggcg acgctcagaa cgctcgacgc
     1441 cttcttgcgc gacatctggc tggagtgctg cggccacctc agcgcgttca ccatcgaaaa
     1501 cgaacaatac gaactgaaca ccggcggtgt ggatgccatg tggcccatga ttttcggccg
     1561 aggcgcgccg ccgaagagca tgaacatccg gctggaggcg gtgctgcgcc ccggcctgaa
     1621 attctttcac gagtacgatt tcggcacgac cacgcatctg gcgctgcgtg tggcggccga
     1681 gcaggaaggc atcgtgcccc gaaacgccgt caggatattg gcccgcaatc atcccccgcc
     1741 gatcccgtgc gagaagtgcg gcgcgccggc caccgaagtg tgcgcgcagt gcatctacga
     1801 cgggcgaggc tgggtgtgcg cctcccacgg tcgcacgcac aaatgcggcg aggacatgct
     1861 gctgcccgtc gtcaattcgc cgcgcgtcgg catgtgtggt tacacgggct agtggctcat
     1921 cattccagtc ccagcatctt cttggtcttg cgatagtcgg gggcaaatcc agggccttga
     1981 taacgttgct gcatgcgctc gccttcgtcg atgagctcga gcacacgccg ccggccttcc
     2041 ggcgttttga cagcctccaa cggtgtcagc ccacccagtg ccgggatcgg ctcgctgatc
     2101 catcgcctga gcaggtgctc ttcgatctgc gccaggactt ccggtggcgg cacaaacggc
     2161 tcgccgggcg gctcggcttg cggcgctcgc tcactcaagg cttgagccgc actgcgggcg
     2221 gatgtctcct ctagccggat gcgatccccc aggagttctt ccagtcgcct gcggcagttg
     2281 gccagtcttt gctccgacat tgcctcaatt tccagcatgg tcggcgtcaa cgtcaggctg
     2341 gcgaggacgc ggtatcccag cgccggatcg gtgacgccgg ggataggctt cgtttgcagc
     2401 caaccgaagg tttgttgcgc ttggcccatt tcctctgggc cgggcgcgtc gaagtcctcg
     2461 gctgcgccca gcacctgccg gatgccctct ggatcggcgt gacgatagcg ggcgctggca
     2521 aagacgagcg gcgtgccttc aggcgtgacc aattggatgc cgcctgtgga tcgtggcgcg
     2581 ggccgccgga ggcggcggtc gatttgtggt ctcttcttgt gcttcttcat ggagggagcc
     2641 tttcagcaat gtaagtttgc gcaccgaaac ttacattgct caactgatgt gtaagttcgc
     2701 tttcattgca cgacgtgaaa gatatcccca acatggacca gtctcagatc gagcgtgaat
     2761 aacaacaaag gcggctctcc aggttggtgg gaggaccgcc gtctgttcat cggttcggat
     2821 acgatttgag attaacagtg ggcggtagag gacttgaacc tctggccttt gcgatgtcaa
     2881 cgcaacgctc taaccaactg agctaaccgc ccaaccaaac gcgattatac tcgcaagcat
     2941 ccgctttggc aagacttcgg aagtctttca gtaagacttc cgaagtctaa aaatcggaac
     3001 tggtgagagc tcagatgacc gattcctcga cgatgggttt gcgagactgc agcaactgcg
     3061 ggctgacgct cagaaggcgg aagacctcgt cgatgtcgcg aagcgtcaac agccctcgga
     3121 attggccctg ctccatcacc ggcagcgctt cgatatgatg ctcggacatc ctctgctgcg
     3181 cgacgtacac gctgtcgtcg agcgtgacgg tgggaaactc cttccgcatc acgctgacga
     3241 cgaaggcgtt cgagccgcga tcctgcagcg cggaaatcag gtcggtcctg gtcagcaacc
     3301 cgaccagctc ctcgccccgg cacactggaa aatcggattg gaacgagctg agcgtcaaat
     3361 cgacggcgcg cgccagccga tcgtccgggt tcagcgcctc ggtcttgcgc gtgaatgcct
     3421 ggcgcacctt caggccgtcc agcacgctct tcacctgcac catctgccct tccgagccgg
     3481 cccccaggaa cacgaagatg gcaatcaaca ccaggttcaa ttggccggtc agaaaaccga
     3541 acaagccggc gagcagtgcc aggttctggc cgatggccac cgcgatcgcc gtcgcccgtg
     3601 agtaattgat gcgcgtcgcc agcagtgccc gcaggatgcg ccctccatcc atcgggaacg
     3661 ccgggatgag attgaagacg gccaggatca gattggtgct gaacaggtag gccagcaggc
     3721 tggccggttc caactgcagc atcccgcgct cgagcgcgcc caggtcgaag ccgacttgaa
     3781 gacgtgccag gacctgcgcc agaacgaaaa tcacggcgac gatcgccacg ttcaccgccg
     3841 gcccggcaat ggcgacccag aattcttgcg ccggcttgtc cgggatgcgc tccagccgcg
     3901 ccacaccgcc gaacggccag agcgtgatgt cgcgcaccgg gatgccgtag cgctgagcga
     3961 cgacgctgtg gcccagctcg tgcagcacca cgcaggcgaa gagcagcagc gtggcgacga
     4021 tgccgaagag cgcgccgttc atcccgcgct catccgaccc gaacacaatc gccgcgtaaa
     4081 tcaggatcaa gaagaacgtc acgtgaatct tgatgtcgat gccgcgcaca gtcgcgattt
     4141 tgaatgatcc gcccatgggt tcccccgttt atgctctgat gattgcacga gtccagttat
     4201 gctgagcgca ctcgaaaatt ccgagtaggg cggctttcca tagccgccat ttcaaaggca
     4261 ggtaagagac ctgccctacg gtgtgcggga gggtcggcag caattatatc ctcttgtgca
     4321 ctactaccgc aacttgcaga cggcagtctg accggctaca atccgagcga tgagcgacgc
     4381 ttcgcacccc acctggccgg tcgtcggcca cgcatgggcc gtagcgcagc tcgcgcgcgc
     4441 gatcgacgag gaccgcttga gccatgccta cctgatcacc ggcccggcgc agatcggcaa
     4501 ggcgacgctg gcgcgagcga tggcgatggc gatcaactgc accggcgagg cgcgcccgtg
     4561 cggcgcgtgc cgcccgtgtg tccgcatcgc gacgagcacg cacgccgacg tgcgcgtcgt
     4621 cgcgccggaa ggcgagcggc tgaagatcga tcagatccgc gagctgcagc gcgagctgtc
     4681 gctctcgccg gtcgaagcgc gcaagcgggt cgccatcctc gacgatttcg accgcgcgac
     4741 gatcgaggcg gcgaacgcgc tgctcaagac gctcgaggag ccggcgtcca gcgtcgtgct
     4801 cgtcgtgatc gcgccggaag ccgagctgct gctgccgacg atcgtctcgc gatgccaggt
     4861 cgtcgcgctg cggccgctga cgatcgcgca ggtgcgcgac gcgctggtga gccggtgggg
     4921 cgtcgacgcc gggcgcgccg acctgctggc gcacctctca ggggggcgaa taggctgggc
     4981 catcactgcc gcgaaggaca aatcagtcct ggagcggcgc gcggcgcgcc tggaccatct
     5041 gcagcgcctg ttcggcgcct cgcgcgtcga gcgcttcgcc tacgccgagt tgctggcgcg
     5101 cagcgcggac acgcgtgaag cgatcgaagt ctggcgcacg tggtggcgcg acgtgatgct
     5161 ggcgtccgcg aacagtggcg ccgagctgac gaacgtagac aggcgcgatg agatcgagcg
     5221 cgtcgccgcg cgcctggatg tgcagcaggc gcgctcggcg gccgaggcgt gcggccgcgc
     5281 gctgtggcag ctcgacaaga acgccgtccc gcgcctggtc gtcgaggtca tgctgatgag
     5341 cctgccaaag atatagcgtg ccgggcgggg ttcgtgttgc gtgttacgtg ttgcgtgttg
     5401 cgtgagagga gggtgcgatg acgttcgacg aatggctggc ttctgtgccg gcggagatca
     5461 ctgacgaccc gctctggcga atggaggtct atcggctcgc gctattcgcc ggcgatcttg
     5521 catggcacga cgtatcccgg ctggctcagg acaagcgcac tatcagcctg tccggccaac
     5581 tgtacgaagc cgtcggttcg ataagcgcca atattgcgga gggctacagt cgcagctctg
     5641 gcaaagatga agccaggttt tatgagtatt cgctgggatc ggctcgcgag gcgcgaacct
     5701 ggtactatca gggccgccat gtgctgggcg ccgtcgtcgc cgggcatcgc atcaaactgc
     5761 tcacgcagat catccggctg ctgctcacga tcatacccgc tgaacgccgt cgtaagctgt
     5821 cagatgaaca agttgcctac gacacaaata tcgcctatct cctcaacaat cctcccatgc
     5881 cataaaacac acgcaccacg taacacgcac cacgcaccac gcaatacgca tcagtgcgcc
     5941 cctactatca cctccatggc agcctccgga tcgagcgcga cgatattccc tccacgaaag
     6001 tcgaacaaca ggcgcccgtc cggcgtgaac gccgcccatg gactgccttc caggccgatc
     6061 gcgctgcccc cgtcaaacac gcgccgcgcc gcgccgctcg ccagatcgat cgacagcaca
     6121 tcgccgtctt gcgcgatggc atatactcga tcgccattcg ccagcaggcg cggcatcgtc
     6181 gtccccaggc cggcgatagc ccagtcccag cgcagcgatc cgtcggcgtc gagcgcgatc
     6241 aagcgccgcg cgaattctcc ccgatgcgag acgacgacac cgccatctgg cagcgcggcg
     6301 atggtgccga tctcgaagac gctcggatca agcggcttca gaagcctggc tgtcaacgtt
     6361 cccggatcga tctggtagat gccgttcggg ttgtagagga acatgcgatc gccagagacg
     6421 acagggcggc cgccgataga cgcacctgcc aagagacggc ccgaccgatc cagcgcgtaa
     6481 agggtgggct gctgagcaac ggtcgtgaag atcagtcgat cgccgtccag cgtccagtcg
     6541 aacggcgggg ccacgttttg gatcagccac aggcgtttgg cgtccgtccc gataccgagc
     6601 aggcgatttt cgttatgcac cacgacaccg ctgcccggca gaggcaacag gtagggtcga
     6661 gcacgggttg cgaattgcgc ctgccaaacc gtttcgccgc tcgcgtcgac ggcctcgacg
     6721 gcgccgcctt gacgcgaaag ataaatcgcc gagccgctcg cgtcgacgac agacgagaga
     6781 atcgttcccg agaccggacg gctccagcgc aggctgccgt cattcggatc gacgacaatg
     6841 aggcgctggc cgtcgagcgc ggcgagcgcg ccgtcgctca gcaggccgat gcccgtcgag
     6901 gcggtcgccg tgaaggggcg cgtccattgg acgccgggcg ctgtggcgat gcgatacgtc
     6961 cagatatatt ccgacggcgg ctgccatccc gccaggcgcg gatcgaccgg ccagtagccc
     7021 ggaccgggat cgtcgggcgt gtggttgcgg acctcgaagt gcagatgcgg cctgccgcgc
     7081 ggcttgccga tcgcgccgac ctggtcgccg cgcgccacgc agtcgccggg ccgcagctcg
     7141 acactgtccg gatcgagatg gccgtagaac gagagaatcg tcccgccgtc ggggaagagg
     7201 tggcgcacga tcacgacgcc gcgatcgacg ccccagccca acggctgtgc atacgtcacc
     7261 gttccgtgac ccacgctgta taccggttgc cccaggctat cgccgttgct gcgcacccaa
     7321 tc
//
LOCUS       DBNHXB010000097         3904 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3708692, whole genome shotgun sequence.
ACCESSION   DBNHXB010000097 DBNHXB010000000
VERSION     DBNHXB010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3708692"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="AC162G_03765"
     CDS             <1..313
                     /locus_tag="AC162G_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5074964.1"
                     /translation="GVPIGNRLIRTIQAWNNVLLLWEAMKRADTAGNLTGEGIRQAFE
                     TIQNYDLGLGASPVTYTASDHRPASAVNIYIIRNGTFELLQRVDLKERWPDLWPTWLG
                     Y"
     gene            356..1156
                     /locus_tag="AC162G_03770"
     CDS             356..1156
                     /locus_tag="AC162G_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5074965.1"
                     /translation="MLQLNNIEVRYHEVILVLKGVSLEVPPGGIVALLGANGAGKSTT
                     LKAISGLLRHEDGAVTDGSIEFMGQRIDRKSAEEIARMGIIQVIEGRRVFEHLTVEQN
                     LKVGAHLRPDGASGGLRDGLEMVYTYFPRLRDIRHKQAGFASGGEQQMMVIGRALMAT
                     PKLMLLDEPSLGLAPLLIQEIFGIITRLNREQGIPIVLVEQNVKLALAVAPYAYVLEN
                     GRIVMDGPADKLRDNPDVKEFYLGLTDLGTRKSFRDVKHYKRRKRWLT"
     gene            1259..1861
                     /locus_tag="AC162G_03775"
     CDS             1259..1861
                     /locus_tag="AC162G_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074966.1"
                     /translation="MNTRRITALLITLICGAALIALGACGAPTPSPALQNPTVGLDHV
                     EVASYFPWPPPPGEGTPAPPPVRVPMVLAFIFDIANPNSYPVMLKDLQYTVEFEAAPN
                     AFFPVGNPFAHEDMSIPANSTNQLRVTMVLDSLVTPGNLAVTSGSRLQEMGLSAGEVV
                     QNWWANVGDFTFKIKVTGGSAEFSSSGGNAVVPFEGEYPK"
     gene            1934..2827
                     /locus_tag="AC162G_03780"
     CDS             1934..2827
                     /locus_tag="AC162G_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5074967.1"
                     /translation="MIDLQDETFDGSFPFAPHYRRIRGFEMHYVDEGSGEPIVLVHGD
                     PTWGYLWRKFIAPLSQRRRCIAPDHMGMGKSGAPDEPYPYRLRHHIANLETLLLDLDV
                     RGMTLVLHDWGGPVGLGFAIRHPERIKRLILMNTWAFAPWPGGPLPRLIELIRSERGE
                     KFVLEKNGYLEPALLGTTTHREHLTEMVLRAYQAPYPTPGSRRALLCWSRDIPVSESD
                     PSYAEMKSIEQGLFQFGKTPTLLVWGMQDPVLSEPVLRMWQRIYPHATTCDIADASHF
                     LQEDAPDRIVSRIEEFLDANA"
     gene            3190..>3904
                     /locus_tag="AC162G_03785"
     CDS             3190..>3904
                     /locus_tag="AC162G_03785"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HIR5074968.1"
                     /translation="MKIVIPGGSGQVGTILARAFHQAGHEVVVLSRKPTNSVWRTVKW
                     DAETLGDWAAELEGAEVVINLAGRNVNCRYTAENRRLIMESRVKSTQIVGRAIAQASH
                     PPRTWLQASTATIYAHRYDAPNDEATGIIGGSEPDAPSAWRFSIDVAMAWERALNDSA
                     TPRTRKVLMRSAMIMSPDRGGIFDVLLRLVRFGLGGRVGDGRQYVSWIHDQDFSRAVL
                     WLIEHEELEGVVNLSAPNPV"
BASE COUNT          766 a         1186 c         1210 g          742 t
ORIGIN      
        1 cggcgtgccg atcgggaacc ggctgatccg caccattcag gcctggaaca acgtgctcct
       61 gctgtgggag gcgatgaagc gggccgacac ggccggcaac ctgaccgggg aagggatcag
      121 gcaagccttc gagacgatcc agaactacga tctcggcctg ggcgcttcgc cggtcaccta
      181 cacggcgagc gatcatcgac cggcctcggc tgtcaacatt tatatcatcc gcaacggcac
      241 gttcgagctg ctgcagcgag tcgacctcaa ggagcgctgg cccgatctgt ggccgacgtg
      301 gctgggttac tagcgcgtgg ggcgtggagc gtgaagcgtg gagcgtgggg cgagcatcct
      361 gcaactgaat aacatcgaag tccgctacca cgaagtcatc ctggtgctca agggcgtatc
      421 gctggaagtg ccgcccggcg gcatcgtcgc tctcctcggc gcgaacggcg cgggcaagag
      481 cacgacgctc aaggctatct ccggcctgct ccggcacgag gacggcgccg tcaccgacgg
      541 cagcatcgag ttcatgggac agcggatcga ccgcaagagc gccgaggaga tcgctcgcat
      601 gggcatcatc caggtgatcg agggccggcg ggtcttcgag cacctgacgg tcgagcagaa
      661 cctgaaagtc ggcgctcact tgcggcccga tggggcctca ggaggcctgc gcgacggcct
      721 ggaaatggtg tacacctatt tccctcgcct gcgcgacatt cgccacaagc aggcggggtt
      781 cgccagcggc ggcgagcagc aaatgatggt catcggccgc gccctgatgg cgacacccaa
      841 gctcatgctg ctggacgaac cctcgctggg attggccccg ctcctgattc aggagatctt
      901 cggcatcatc acccgcctga atcgcgagca gggcatcccg atcgtcctgg tcgagcagaa
      961 cgtgaagctg gcgctggccg tcgcgcccta cgcctacgtg ctggagaatg gccggatcgt
     1021 gatggatggt ccggctgaca agctgcgcga caatccggac gtcaaagaat tctatctcgg
     1081 cctcaccgac ctggggacgc gcaagagctt ccgagacgtg aagcactata agcggcgcaa
     1141 gcgatggctg acatagtcgc gaagcgtgga gcgtggggag acccttcgac tgcgctcagg
     1201 gcaggccctg cgctcgctcc gggtcgggag gctcatcacc aagaaagggg ccgaacacat
     1261 gaacactcga cgaatcacgg cgcttctcat aaccctgatc tgcggtgcgg cgctgatcgc
     1321 gctcggcgcg tgcggcgcgc ccacgccttc ccccgcgctt cagaacccga ccgttgggct
     1381 cgatcacgtc gaagtcgcca gttacttccc ctggccgccg ccgcccggcg agggaactcc
     1441 cgccccgccg ccggtgcggg tgccgatggt actggccttc attttcgaca ttgccaaccc
     1501 caactcctat cctgtcatgc tgaaagacct gcagtacacg gtcgaatttg aggctgcccc
     1561 gaacgcattt ttcccggtcg gcaacccctt cgcgcacgaa gacatgtcca tcccggccaa
     1621 ctccaccaat caactgcgcg tgacgatggt gctcgactcg cttgtcacac ctggcaacct
     1681 ggcggtgaca tcgggttctc gtttgcaaga gatgggcctg agtgccggcg aggtggtcca
     1741 aaactggtgg gcgaacgtcg gggactttac atttaagatt aaggtgaccg gtgggagcgc
     1801 tgaattctcc agttccggcg ggaacgcggt cgtgcctttc gaaggcgaat atcccaagta
     1861 gcagacggca agccagcctc ccgaaggatg tgaattcttc gggaggtttt cacaatggag
     1921 cgtggagagg gacgtgatcg acctgcaaga cgagacgttt gacggctcct tccccttcgc
     1981 cccccactat cgaaggatcc gcggctttga aatgcactac gtggatgaag gcagtgggga
     2041 acccatcgtg ctggtacacg gcgacccaac ctggggctac ctgtggcgca agttcatcgc
     2101 tcccctctct cagcgccgcc gctgtattgc gccggatcac atgggcatgg gtaagtcggg
     2161 tgcgcccgat gaaccctatc cgtaccgtct tcgccaccac atagccaacc tcgaaaccct
     2221 tttgctcgat ctggacgtgc gcggcatgac gctggttctt cacgactggg gcgggccggt
     2281 agggttggga tttgcgatcc gccaccctga gcggatcaag cgcctcatcc tgatgaacac
     2341 atgggctttc gccccatggc cgggcggtcc actccctcgc ctgatcgagc tcatccgctc
     2401 cgagcgtggg gaaaagttcg tgttggaaaa gaacggctac ctggagcctg cgctgttggg
     2461 gacgacaacc catcgagagc acctgacgga gatggtccta agggcctatc aagccccgta
     2521 tccgacgcct ggctcacgcc gcgctttact gtgctggtcg cgcgacattc ctgtcagcga
     2581 atcggatccg tcctatgccg agatgaagtc gatcgagcag gggttgttcc agttcggcaa
     2641 gaccccaacg ctcctggtgt ggggcatgca ggacccggtg ctctccgaac ccgtcttgcg
     2701 catgtggcag cgcatctacc cccacgcgac cacctgcgat attgcggacg ccagccactt
     2761 cttgcaggaa gatgcgccgg atcggatcgt aagccgcatc gaagagtttc tggacgccaa
     2821 tgcttgacca cggatttcca cccccgcaca cggcaggcga gggcaagcgg attccacgga
     2881 tatcatgaga ggccgccatc cgtggtgaag aatcttcgcg gttgcgggca gtgacctggc
     2941 cggtctcgtc cagagaacgt acgtcatgtt ggggttgact ccagtcgatt ccgatgtatc
     3001 ctgttaaaag gacctttctg tgctgactcg ggtcctctgc tcccaggccg gggtagctga
     3061 tgggggtggc agacgctgat gggttatcac cccgttctgc acgaagcata ccccagtcgg
     3121 cctttgtcga gtataaggta acaggcttag ctctgcattg ggcggtggac atcgtagagg
     3181 cgtagcagcg tgaaaatcgt cattccaggt ggctcagggc aagtcggaac aattctcgcg
     3241 cgtgccttcc accaagccgg gcatgaggta gttgttctca gccgcaaacc taccaactcg
     3301 gtttggcgca cagtgaaatg ggatgctgag acactgggtg actgggccgc agaacttgaa
     3361 ggagccgaag tcgtcatcaa tctagcggga cgtaatgtaa actgccgcta caccgccgag
     3421 aatcgtcgcc tcatcatgga gtcgcgcgta aagtccactc agatcgtggg tagggcaatt
     3481 gctcaagcgt cacacccgcc ccgaacatgg cttcaggcta gtacagcgac aatctacgct
     3541 catcgctatg atgcacctaa cgacgaggca actgggatca taggtggctc ggaaccggac
     3601 gcgccatccg cctggcgctt cagcatcgat gtggcgatgg cttgggagcg cgcgctgaat
     3661 gactcagcga cgcctcgcac ccgcaaggtg ctcatgcgtt cggcgatgat catgagccca
     3721 gatcgcggag gcatctttga cgtgctgctg agattagtgc gattcggcct aggtgggcgt
     3781 gtcggagatg gccgtcagta cgtatcttgg atacatgacc aagacttctc gcgagccgtt
     3841 ttgtggttga ttgagcacga ggaactcgag ggcgtcgtca atctctctgc acccaacccg
     3901 gtgc
//
LOCUS       DBNHXB010000098         2541 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1863325, whole genome shotgun sequence.
ACCESSION   DBNHXB010000098 DBNHXB010000000
VERSION     DBNHXB010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1863325"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..2531
                     /locus_tag="AC162G_03790"
     CDS             66..2531
                     /locus_tag="AC162G_03790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR5074969.1"
                     /translation="MPTGIGMPFLQNARRMTRAHPYGLGLAVLLSLFFLQAALASPRN
                     SVTFDEQYHVTLGYSYLKTGDLRLHRNQSPPLFEIITSLPLLLRSDLNLPLDHPAYAL
                     WGDVYLFSDVFLWHANADAQGMVHTGRLVAAGFGVLLGLLLYLWGKQLYGAKAGLLAT
                     FLLALDPNFIANAIPTLDLGLAFFVTLAAWRLWHSLRRPTWLNILLAGLALGAALSTK
                     FIAVTLLPAFALILLVYPRQIAKTQSHREDFVASWLRGEIARRALSFAVMLVIAWLVI
                     WIVYGFQFGPIDGAGTPVPAPTYFESFVGMLAKSSEGRMNYLDGELSPTGWPHYFVAA
                     MLYKTPLPVLLAALVALIRWPWRDEWRETSILIVPAALFFVAASFGNLQLGYRYILPV
                     LPFVYLIASRVVTFTWPRWAVVPAAALAMWLIAGTLSIFPHYLSYFNEVAGGPQAGYQ
                     HLVDSNLDWGQDLPALKDWIDAHRPENLHLGYFGTAYPDRYGVRAIGIPGYPLNAFGS
                     EVDGFTSYSLEAGLYAVSATALRIGMVFDRFNIYEAFESMTPIDRAGYSILIYDRRYP
                     PGADVDRAVIAGPRAADVPPQALGVRPDPSTTLRTSHPLRAKWCAWPECFVFTPHPAR
                     YIVRDFIPFAPDLAEQAHARAVPVDSLRLGDDAYAVSELDATSIIQSKLNALRASVFA
                     APDETPLSPPIAFDNGLSLVGYEVGSDRVTPGRVLDAITYWQVTDRISPPMTIFVHLL
                     DSQGNIRGQHDGFGAALTTLEPGDVVAQHHVIPVDASVEPGTYRLQVGLYNPITLERF
                     AARPGEVAPGDRVLLSTIEVVAP"
BASE COUNT          393 a          854 c          773 g          521 t
ORIGIN      
        1 gatcgaatcc gaccacgtct cactcggacc gatcgagatc gattccgccg cgctcaggtg
       61 ataccttgcc gaccggcata gggatgccgt tcctccagaa cgcacgtcgc atgacgcgcg
      121 cgcacccgta cgggttaggg ctggctgtcc ttttgagcct cttctttttg caagcggcgc
      181 tcgcctcacc gcgcaactcg gtcacgttcg acgagcagta tcacgtcacg ctgggctact
      241 cgtacctcaa gaccggcgac ctgcgcctgc accgcaatca aagcccgccg ctattcgaga
      301 tcatcacgtc cctgccgctg ctgctgcgat ccgatctcaa cctgccgctc gaccatccgg
      361 cctacgcgct gtggggtgac gtgtacctat tctcagacgt cttcctgtgg catgccaacg
      421 ccgacgccca gggcatggtc catacgggga gactcgtcgc cgccgggttc ggcgtgctgc
      481 tcggcctatt gctatacctg tggggaaaac agttgtatgg cgcgaaggcc gggttgctcg
      541 ccaccttcct attggcgctg gatcccaact tcatcgcgaa tgccatcccc acgctcgatc
      601 tgggtctggc tttctttgtc acgctggcgg catggcggct gtggcactct ctcagacgtc
      661 caacctggct caacattctg ctcgccggac tggcgctggg cgcggcgttg agcaccaagt
      721 tcatcgccgt cacgctgctg cccgcgttcg cgctgatcct gctcgtgtat cctcgacaaa
      781 tcgcaaagac acagagtcac cgagaagact tcgtggcttc gtggcttcgt ggtgaaattg
      841 cccggcgcgc gctttccttc gcggttatgc tggtgatcgc gtggctcgtc atctggatcg
      901 tatacggatt ccagttcggc ccgatcgacg gcgccggcac gccggtgccc gcgccgacgt
      961 acttcgaatc gttcgtgggc atgctcgcca agagcagcga aggccgcatg aactacctag
     1021 acggtgagct gtcgcccacc ggctggccgc attacttcgt cgccgcgatg ctgtacaaga
     1081 cgccgctgcc tgttctgctg gccgcgctcg tcgcgctgat ccgctggccg tggagggatg
     1141 agtggcgcga gacgagcatc ctgatcgtgc cggcggccct gttcttcgtc gccgcatcgt
     1201 tcggcaacct gcaactgggc taccgctaca tcctgccggt gctgccgttc gtgtatctca
     1261 tcgcgtcacg cgtcgtcacg ttcacatggc cgcgctgggc cgtggtcccg gcggccgcgc
     1321 tggcgatgtg gctgatcgcc ggcacgctgt cgatcttccc gcactatctc tcgtatttca
     1381 acgaagtggc aggcgggccg caggccggtt atcagcacct ggtcgattcg aacctcgact
     1441 ggggacagga tttgcccgca ctcaaggact ggatcgatgc gcatcggccc gagaatctgc
     1501 acctcggtta cttcgggacg gcctatcccg atcgctacgg cgtgcgtgcc atcggcatcc
     1561 ccggttatcc gctcaacgcc ttcggcagcg aggtcgacgg cttcacgtcg tacagtctcg
     1621 aagcgggcct gtatgccgtc agcgcgacgg cgctgcggat cggcatggtc ttcgaccgct
     1681 tcaacatcta tgaagcgttc gagtcgatga cgccgatcga tcgggccggg tactcgatcc
     1741 tgatctacga caggcgatac ccgcccggcg cggacgtcga ccgcgccgtg atcgccgggc
     1801 cgcgcgcagc cgacgtgccc ccccaggcgc tcggcgtgcg tcccgatcct tcgactacgc
     1861 tcaggacaag ccatccgctg cgcgccaagt ggtgcgcctg gccggagtgt ttcgtcttca
     1921 cgccgcatcc ggcgcgctac atcgtgcgtg acttcatccc gtttgcgccg gacttagccg
     1981 aacaggcgca cgcgcgggcg gtgccggtcg attcacttcg cctcggcgat gacgcatatg
     2041 ccgtgtcgga actggatgcg acctcgatca ttcaaagcaa gctgaacgcg ctccgtgcat
     2101 cggtcttcgc cgcgcctgac gagacgccac tctcgccgcc gatcgcgttc gataatggcc
     2161 tgtcgctggt gggctacgag gtgggcagcg accgcgtcac tcctggccga gtgttggatg
     2221 ccatcaccta ctggcaagtg accgatcgca tctcgccgcc gatgacgatc ttcgtccatc
     2281 tgctcgactc acagggaaac atccgcggcc agcacgatgg cttcggcgcg gcgctcacca
     2341 cgctcgagcc gggcgacgtc gtggctcagc accacgtcat cccggtggat gcgagcgtgg
     2401 agccgggcac ctatcggttg caggttggcc tgtataatcc catcacgctc gagcgttttg
     2461 cggcgcgtcc aggggaggtg gcgccgggcg atcgggtgct attatccacg atagaggtcg
     2521 tcgcaccgtg atgcgcttcc t
//
LOCUS       DBNHXB010000099         5737 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_689267, whole genome shotgun sequence.
ACCESSION   DBNHXB010000099 DBNHXB010000000
VERSION     DBNHXB010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_689267"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..458
                     /locus_tag="AC162G_03795"
     CDS             249..458
                     /locus_tag="AC162G_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074970.1"
                     /translation="MSVPPHTIDPEQVIERIDAAIRELEALRRQFIVPTPTMPDLAEK
                     LFGALGQGTWDEYDSHLDWARFDAG"
     gene            455..946
                     /locus_tag="AC162G_03800"
     CDS             455..946
                     /locus_tag="AC162G_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR5074971.1"
                     /translation="MSALLSTFDEQVIYLDTMLPYALLRRIDPAVKSFFARIEQGAIR
                     AYTSVVTFDELAYRLVLALIKDRYGGSPLDRLRDDEEKMLAEYVPAIAPQLRRLRDFP
                     NLTVIEVSIVDVETMIGAMEQYRIRPRDALHFAAMRRVDCVNLASNDQHFDRIPAIRR
                     FAL"
     gene            1016..1291
                     /locus_tag="AC162G_03805"
     CDS             1016..1291
                     /locus_tag="AC162G_03805"
                     /inference="COORDINATES: protein motif:HMM:NF023857.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HIR5074972.1"
                     /translation="MDADRPTPPTDSLEAVAEFWDTHSLADYWDQTEPAQFEFDPQAR
                     RHYYVAIDPELMARLHDVARRKGLHMENLINLWLEQRLREAATSSTS"
     gene            complement(1499..1966)
                     /locus_tag="AC162G_03810"
     CDS             complement(1499..1966)
                     /locus_tag="AC162G_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR5074973.1"
                     /translation="MLSNDFIAKYPHLFQEVIWPWGPTRARFVLLESIPSNRQIANVN
                     IGPRTGNGWVTIRLKDGSWEIPGGTLKPHEEYIDTARRELMEEVGAQLTSRDGHRSVH
                     ISRAIRHRRTLSVGIRHRIRCERTELTQSDVTESDTPAARRRRAGHFVAAGKS"
     gene            2201..3757
                     /locus_tag="AC162G_03815"
     CDS             2201..3757
                     /locus_tag="AC162G_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074974.1"
                     /translation="MIERATQSPEYWGQSFSVQPDDLDHLLNLFLEDEMPRSADELAL
                     ALVRYRCQREESALSRELVKGKLYQPKDSYAVGDQVVFPALKFAAGTVIAVRDGVNPE
                     IGPFKVIRVELDNGHTREFAADYSAPHRLNTDGGLAAGEALLSPEELFKRHGRPVVDK
                     LAQKLNESPDLVRLAGKWFLRSLIADVNVGHLNLAEAVLDMHQGGPLPTEALLKDIGM
                     PEQINRHLQIFSLNHALQRDERFDEVGPAGEVLWFLRRLEPPEVLFQPPRLIAAGAPS
                     DRSRLDDALLRIEREIDDELSAIPAPDDDYDEVTFVLTYPHRRVGTVPLSPRIARLFP
                     TGRTHRIRFMFEDARTGQRWPGWVVREHAYAFGLDTWYEANDVPAGAYVQIRRADEPG
                     VVAVDLKGRRPKREWVRVTHVKDRRVTFEMVKRQIGCDYDELMMLVVDDPAVIDEAWL
                     RLAGEELSHLIGALFPELAKLSPQGTVHAKSLYAAMNALRRIAPGPLFAAWSAHPAVR
                     IVGDGYGVSR"
     gene            3901..4341
                     /locus_tag="AC162G_03820"
     CDS             3901..4341
                     /locus_tag="AC162G_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074975.1"
                     /translation="MPHPPKKLSRQIKPALDTPFHIDFEWWQREGRDLHAYLLSQIPA
                     DLREAYAELVDGATVDTVNPETGEVRQEDGLLRRVRAIAKQQADFVNQHTSIIDAVFR
                     TFLVNDNKPLTSYELSRHLNRDAELILRTLSGAQVYKGLRPCTE"
     gene            4350..4589
                     /locus_tag="AC162G_03825"
     CDS             4350..4589
                     /locus_tag="AC162G_03825"
                     /inference="COORDINATES: protein motif:HMM:NF025807.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR5074976.1"
                     /translation="MRVNVKLHAVLRDYRPPDCKGDVMLVDLDDGATVLKVLEHIGIP
                     RKRLHAAFLNDEQVGVDAVLRDGDFLRLFPPVAGG"
     gene            4624..4696
                     /locus_tag="AC162G_03830"
     tRNA            4624..4696
                     /locus_tag="AC162G_03830"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4657..4659,aa:Arg,seq:tct)
     gene            4871..5350
                     /locus_tag="AC162G_03835"
     CDS             4871..5350
                     /locus_tag="AC162G_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIR5074977.1"
                     /translation="MPQSSIPGETRQQIEQLAQDLLARYHVTRPPVPVERMVTEPSLG
                     LWNAKPTELSFTIGHGLYRYAPRMAQARLLYRAICDSDTARQHGLNVPWPASRHEIKY
                     FARCLLMPEAWVHALPKSERHPDRLSERFQVPPFDAVTRLAELGLPIPDDVVPGLDT"
     gene            5380..>5737
                     /locus_tag="AC162G_03840"
     CDS             5380..>5737
                     /locus_tag="AC162G_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HIR5074978.1"
                     /translation="MPHPFPSAEWVADFERQINSDEEYARAASTWEGDLNFVIEDVPG
                     QDTPVVVYVDLWHGKCRDARFTDLASAPDARFNINAPLANWKRVITKQIGPIPAMVSG
                     QLKVRGNLPYILRHVSA"
BASE COUNT         1100 a         1844 c         1703 g         1090 t
ORIGIN      
        1 ggggatacct gagacctcca ggcggccctt gatcacctgg gccttcagca agcccgacgc
       61 gcgatacaca acctccaggc cctcacgctt cacgttgcgc ggtccgaaca tgtcgacctc
      121 tgagctcttc agcgtggatc tccggcagcg tggaaagtat agcacaccgc ccgtgccgat
      181 gaggttccct gcccaggcca ttccgaatct aggctataat agcacaacca aggctaggag
      241 ccgttgccat gtcagtgcca cctcacacta tcgatccaga gcaggtgatt gaacgaatcg
      301 atgccgctat ccgcgaactc gaagcgctac gccgccagtt catagttccc acaccgacca
      361 tgcccgacct cgccgagaag ttgttcggtg cgctcggaca aggcacgtgg gatgagtatg
      421 actctcacct cgactgggcg aggtttgacg ccggatgagt gcacttttga gcaccttcga
      481 cgagcaggtg atctacctgg acacgatgct gccatatgct ttgctgcgca ggatcgaccc
      541 ggccgtcaaa tcgttcttcg cgcgcatcga acagggtgcg attcgcgcgt acacatccgt
      601 ggtgacgttc gacgaactgg cgtatcgcct cgtattggcc ctgattaagg atcgctacgg
      661 cgggtcgccg ctcgaccgtc tgcgtgacga cgaagagaag atgctggccg aatatgtgcc
      721 ggccattgca ccccaattgc gccggctgcg cgactttcct aaccttacag tcatcgaagt
      781 aagtatagtt gacgtggaga ccatgatcgg ggcgatggag caatatcgca tccgtcctcg
      841 cgacgcgctc catttcgccg ccatgcgacg agtggactgc gtcaatctgg caagcaacga
      901 ccagcacttt gatcgcatcc ctgctattcg ccgattcgcg ctctgactgt gcggccgttc
      961 aagtgcgcag ggaataacat cacgccatca ccctcaccca ataggtccat atcgcatgga
     1021 cgccgataga cccactcctc ccacagacag tctggaggca gtcgccgagt tctgggacac
     1081 gcatagcttg gccgactact gggatcagac cgaaccggcg cagttcgagt ttgatccgca
     1141 agcgcgtcgg cattactacg tggcgattga cccggaattg atggcccgcc ttcacgacgt
     1201 ggcccgccgc aaaggactgc acatggagaa cctgatcaat ctgtggctcg agcagcgcct
     1261 gcgggaagcg gccacatcgt caacgtcatg aagtcagagc acgcttgacg gactggttgg
     1321 cgacccgagt gacaacccgt ccggcttctg tgcatcgcgg gagccgaact gtctatttgt
     1381 gcatgtttac ggcgttcctg gccgaccaac cgggcataat cctgcggcaa gccagcgatg
     1441 ttcttgatcg cccgccgcag gatgcggacg tcatacatcg attccttccg tgcaaagctc
     1501 agcttttgcc tgcggccacg aaatggcctg ctcgccgcct tctcgcagcc ggtgtatcgc
     1561 tttcggtgac atcagattgc gtcaactctg tcctttcgca cctaattcga tgtctgatgc
     1621 caactgatag agttctgcga tgtcgtatcg ccctgctgat atgaaccgag cgatggccgt
     1681 ctctagaggt aagttgtgcg cccacttctt ccatgagttc acggcgcgca gtgtctatat
     1741 actcttcgtg aggtttaagg gtgccgccgg ggatttccca tgaaccatcc ttgagtcgaa
     1801 tggttaccca gccgttaccc gtgcgaggcc caatgttgac gttggcgatc tgacgatttg
     1861 aagggatgct ttctaataaa acgaacctgg cacgagttgg cccccaaggc catatgactt
     1921 cctgaaaaag atgcggatac ttagcgatga agtcgtttga cagcataaat acaatctatg
     1981 acaactcacc acccaggtca agtttgaggt atcaggtatc acatcgtcac gtatcacgca
     2041 tcacgtacca cgcatcacgt atcacgtgca ttccatcgac agacctcgca tgctcaaccc
     2101 gggcttcgag gatcgacgag gtgcgccgcc agacgcattg ttgtggtata atctccccgc
     2161 gcagcaaccc ttcgcctttc gtccgcagga gatccggtcc gtgattgagc gcgccacaca
     2221 gtcgcccgag tactggggac agagtttctc cgttcaaccc gacgacctcg accatctgct
     2281 caacctcttt ctcgaagacg agatgccgcg ctcggccgac gagctggcgc tggccttggt
     2341 tcgctatcgc tgccagcgcg aggaaagcgc gctcagccgc gagctggtca agggcaagct
     2401 gtatcagccc aaggatagct atgccgtcgg cgaccaggtt gtcttccccg cgctgaaatt
     2461 cgccgccggc acggtgatcg ccgtgcgcga cggcgtcaat cccgaaatcg gtcctttcaa
     2521 agtcatccgc gtcgagctag acaacggcca cacacgcgag ttcgccgcag actattccgc
     2581 gccgcatcga ctcaacacag acggcggcct ggccgccggc gaggccctgc tgtcacccga
     2641 agagcttttc aagcgccacg gccggccggt cgtggacaaa ctcgcgcaga agctgaacga
     2701 atcgcccgac ctggtccgtt tggcagggaa gtggttcctg cgcagcctga tcgccgacgt
     2761 caacgtcggg catctcaacc tggccgaagc cgtcctcgac atgcaccagg gcggcccgct
     2821 gccgaccgag gcgctgctca aagacatcgg catgccggag cagatcaacc ggcacctcca
     2881 gatcttctcg ctgaatcacg cgctgcagcg cgacgagcgc ttcgacgaag tcggtccggc
     2941 cggcgaagtt ctgtggtttc tgcgacgcct ggagccgccg gaagtgctct tccagccgcc
     3001 gcgcctgatt gccgccggcg cgccgtccga tcgatcgcgg ctcgacgatg cgctgctgcg
     3061 catcgagcgc gagatcgacg acgagctttc ggcgatcccc gcgcccgacg acgattacga
     3121 cgaggtcacg ttcgtcctga cctatccgca ccggcgcgtc ggcacggtgc cgctttcgcc
     3181 gcgcatcgcc aggctgttcc cgacgggacg cacccatcgc attcggttca tgttcgaaga
     3241 cgcgcgcacc gggcagcgct ggccgggttg ggtcgtgcgc gaacacgctt acgccttcgg
     3301 tcttgacacc tggtacgagg ccaacgacgt gccggccggt gcatacgttc agattcgccg
     3361 cgccgacgag ccgggcgtcg tggccgtcga tctcaagggc cgacgaccga agcgcgagtg
     3421 ggtgcgtgtc acgcacgtta aagatcgccg cgtcacgttc gagatggtca agcggcagat
     3481 cgggtgcgac tacgacgagc tgatgatgct ggtcgtcgac gatccggccg tgatcgacga
     3541 agcgtggctg cgcctggccg gtgaggagct gtcccatctg atcggcgccc tcttcccaga
     3601 gctggccaag ctcagcccgc aaggcacggt gcatgcaaag tcgctgtacg cggcgatgaa
     3661 cgccctgcgg cgtatcgcgc ccggcccgct gttcgccgcg tggagcgcgc atccggcggt
     3721 gcgcatcgtg ggagacgggt acggcgtctc acgctgacgg actgagcctg ttttccacag
     3781 ctcgcgccgc agtgcaactg ccttctcatc ttgcatggac actcatcgcc tgagtgcgat
     3841 cagtggtata atcactcgcg gcactgtgtg ccgcgagttt tctttcgaag ggattgctcc
     3901 atgccccacc cgcccaagaa gctgtctcgc cagatcaagc ccgcgctcga cacgccgttc
     3961 cacatcgact tcgaatggtg gcagcgggaa gggcgcgatc tgcacgcgta tctgctgagc
     4021 cagattcctg cagacctgcg cgaagcgtac gcggagctgg tcgacggcgc cacggtggat
     4081 acggtcaatc ccgagacggg cgaagtgcgg caggaggacg gcctgctgcg gcgcgtgcgg
     4141 gcgatcgcca agcagcaggc ggatttcgtg aaccagcaca cgtcgattat cgacgcggtc
     4201 tttcgcacgt tcctggtcaa cgacaacaag ccgctcacgt cttacgagct gtcccgccac
     4261 ctgaaccgcg acgccgagct gatcctgcgc acgctgtccg gcgcgcaagt gtacaaaggg
     4321 ctgcgccctt gcacggagta ggaaccgccg tgcgtgtgaa cgtgaagctg cacgccgtgc
     4381 tgcgcgacta tcgcccgccg gactgcaaag gcgacgtgat gctcgtggat ctcgacgatg
     4441 gcgcgaccgt gctcaaggtc ctggagcata tcggcattcc gcgcaagcgt ctgcacgcgg
     4501 ccttcctcaa cgacgagcag gtcggcgtgg acgcggtgct gagagacggc gactttctcc
     4561 ggcttttccc gcccgtggcg ggcggataac ctcaccccca ccaccggggt ggaggtgagg
     4621 cttgcccctg tagctcaatg gatagagcgc ctgccttcta agcaggtggc tgtgggttcg
     4681 aatcccgccg ggggcgcctg acaggcgtat tgcctctttc cgattttcgt aggggcgacc
     4741 cctgcgaagc gctccgagcg aagcgagcga gtggtggtcg ccctgggcaa gcgcaaggcc
     4801 tgctcctacg caattcggaa ttttctttag cggtcagtat acgattgtct ggagcaaagc
     4861 catcgcgaca ttgccccaat cgagcatccc cggcgaaacg cggcagcaga tcgagcagct
     4921 cgcccaagac ttgctcgcgc gttatcacgt cacgcggccg cccgtgcccg ttgagcggat
     4981 ggtaaccgag ccgtctctcg ggctgtggaa tgccaaaccc accgagctga gcttcacgat
     5041 cggtcacggc ctgtatcgat acgcgccgcg catggctcag gcgcgcctgt tgtaccgcgc
     5101 catttgcgac agcgacacgg cgcgccagca cggcctgaac gtgccgtggc cggcgtcgcg
     5161 ccacgagatc aaatacttcg ctcgctgcct gctcatgccg gaggcctggg tacacgcgct
     5221 gcccaaatcc gaacggcacc ccgatcggct cagcgagcga ttccaggtgc ccccgttcga
     5281 cgccgtgacg cggctggccg agctgggcct gccgattccc gacgatgtcg ttccggggct
     5341 tgatacctaa agccaatacc gttcaagcta gggagcagca tgccacatcc attccccagc
     5401 gccgagtggg tcgccgattt cgagcgccag atcaattcgg acgaagaata cgccagagcg
     5461 gctagcacat gggaaggcga tctcaacttc gtcatcgagg acgttcccgg ccaggacacg
     5521 ccggtggtcg tctacgtcga cctgtggcac ggcaagtgcc gtgacgccag gttcaccgac
     5581 ctggcttcgg ccccagacgc caggttcaat atcaatgcgc cgctcgccaa ctggaaacgc
     5641 gtcatcacca agcagatagg gccgatcccg gcgatggtga gcggtcaact caaagtccgc
     5701 ggcaacctgc cgtacattct gcgacacgtc agcgcgg
//
LOCUS       DBNHXB010000100         1815 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_857063, whole genome shotgun sequence.
ACCESSION   DBNHXB010000100 DBNHXB010000000
VERSION     DBNHXB010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_857063"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1815)
                     /locus_tag="AC162G_03845"
     CDS             complement(<1..>1815)
                     /locus_tag="AC162G_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012403.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HIR5074979.1"
                     /translation="RGNRSLGVLSTRLYPYQEEGAMHLAFGRRAMLADDMGLGKCVGR
                     DTPLFVNGALHRAEEVWEQFAGPSVFDGEGEWAEPCEPLWTNALTFGEQTEQIVPARI
                     ARLYRQHVAEPMRLIRLSDGSQIVITRRHELLARDRWDNQLEVGDIVCVPARLNWDGP
                     EADPNLVTLLAWQIAEGYELAERGTVRIYQKDVARLEMLRRAAQRFGEKSGIPMNSLG
                     ISQPAGRVAYLTIVSRAYQRFLQVRGYPWGQKSAGKRVPDLIMSSGIETARAFLREYF
                     SAEGSVSTTMRLVEISSASEWLMQQLSCLLRRFGIWLRITVKQKRATNGSGILRTYYI
                     GTLGGNAARLFLSEIGFADDAKQHKLEVICATPANTNVEGIPASDLLTGMADTAGLPY
                     RHFGVGPVYFAGSQELSRPSAHAVIDALDRILTGEAEADYRRLPTSKWTVSTLAAYAA
                     LDHDALQGTRDHLSRLIEREVFYCRIVEIRDVEYGGWVYDFGVEGRHNFVAGGLLCHN
                     TVQAITASALLKELRGIERALVICPASLKHQWAREIRRFTSLPVTVVEGGLNARRDLY
                     REPSFFKIINYEIVRRDLKDLDQLRPDLIVLDEAQRIK"
BASE COUNT          350 a          559 c          556 g          350 t
ORIGIN      
        1 tcttgatgcg ctgcgcctcg tccaggacga tcaggtcggg ccgcagttga tccaggtctt
       61 tcagatcgcg ccgcacgatc tcgtagttga tgatcttgaa aaacgacggc tcgcgataca
      121 gatcgcggcg ggcgttcagg ccgccctcga cgacggtgac cggcagcgag gtgaagcggc
      181 ggatctcgcg cgcccactga tgtttgagcg aggccgggca gatgacgagc gcgcgctcga
      241 tgccgcgcag ctctttgagc agcgccgagg cggtaatcgc ttgcacggtg ttgtgacaca
      301 gcagcccacc agcgacgaaa ttgtggcgcc cttcaacccc aaagtcgtac acccagccac
      361 cgtattccac atcgcggatc tcgacgatgc ggcagtagaa gacctcccgt tcgatcagcc
      421 tcgacagatg atcgcgggtg ccttgtagcg cgtcgtggtc gagggctgcg tacgctgcca
      481 gggtggagac cgtccacttc gaggtcggga gccggcgata gtccgcctcg gcctcgcctg
      541 tcagaatgcg atcgagagca tcaatcaccg catgggcact gggcctcgag agttcctggc
      601 tgccagcgaa gtagacaggt ccaacgccga agtgacgata gggcaatccg gctgtgtcgg
      661 ccataccggt cagcaggtct gaagcaggaa tgccttccac gttggtattc gccggcgtcg
      721 cacagatgac ttccagtttg tgctgcttgg cgtcgtcggc gaaaccgatt tcgctcagaa
      781 agagtcgtgc ggcgttgcct cctagcgtgc cgatgtagta ggtgcgcaaa atacccgatc
      841 cgtttgttgc ccgcttctgc ttgacggtga tccgaagcca gatgccgaaa cggcgcagca
      901 ggcaactcaa ctgttgcatt agccattcag aggcagaaga gatttccacg agacgcatcg
      961 tcgtagagac cgagccttcg gcagagaaat actcgcgcag gaaggcgcgt gccgtttcga
     1021 tcccgctgga catgatgaga tcaggtacgc gcttaccggc cgacttttgt ccccacggat
     1081 agcctcgcac ctggagaaac cgctgatagg cgcggctgac tatagtgaga taggcaaccc
     1141 ggccggctgg ctggctgatg cccaaactgt tcatgggaat gccagacttc tctccgaagc
     1201 gctgcgcggc ccggcgcagc atttccagcc gcgcgacatc cttctggtaa atgcgaacgg
     1261 ttccccgttc ggcgagttca tagccttccg caatctgcca ggccagcagt gtcacgaggt
     1321 tgggatcggc ttctgggccg tcccagttca gccgggccgg cacgcagacg atgtcgccga
     1381 cctccagttg gttatcccat cgatctcggg caagtagctc gtgtcgccgg gtgatcacga
     1441 tttggctccc gtcgctgaga cggatcaatc gcatcggttc ggcgacgtgc tgccggtaaa
     1501 gccgcgcgat tcgagccggc acaatctgtt cagtctgctc gccaaaagtc agcgcattcg
     1561 tccacagtgg ctcgcacggt tcagcccatt cgccctcgcc atcaaacacg gatggcccag
     1621 cgaactgttc ccaaacctct tcagcgcggt gaagcgcgcc gttgacgaac agaggtgtat
     1681 cccgaccaac acacttgcca aggcccatgt cgtcggcgag catcgctcgg cggccgaagg
     1741 ccaggtgcat tgcgccttct tcctggtacg ggtacagccg ggtcgacagc acgccgagcg
     1801 atcggttgcc gcgct
//
LOCUS       DBNHXB010000101         8276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1361057, whole genome shotgun sequence.
ACCESSION   DBNHXB010000101 DBNHXB010000000
VERSION     DBNHXB010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8276
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1361057"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="AC162G_03850"
     CDS             complement(<1..183)
                     /locus_tag="AC162G_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074980.1"
                     /translation="MSISAKGRDLAEIGKIDAYPHRGLLATSDCPAGAIFADEYRIPL
                     DPDAQRPTLVRAHINLI"
     gene            complement(220..1758)
                     /locus_tag="AC162G_03855"
     CDS             complement(220..1758)
                     /locus_tag="AC162G_03855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR5074981.1"
                     /translation="MKPTHRRSIRPERLVVVAFVLLSCLYSVVMPIFEASDELSHFAV
                     VKHMADGLGLPVQRPGVKTAWEQEGSQPPLYYLLVSPIARWIDTSDVSERLRYNPHAQ
                     PGDPYLYANRNLVVHSDAENAPWQGMTLAVHLIRFVSILMGTTTVVLVGVLMREIAPG
                     RPDLATGAMAIAAFTPMFLFITASVNNDNLLILVSTIALVLTMRILNERRYGSREAEP
                     DQRRWLIRRAALAMTIGLAALSKVSGLTLLVPAAITLSVVHLPKREWTRWVGSGLMLA
                     AGVALIAGWWYARNYELYGEWLGIETMADVAGRRSMTLLQLIPELEGFKIAYWALFGA
                     VNILTLPLAYVFYDMLTVLAALGAALYVRRVWREGDRSRLWPWLVLALYVLTVFVGIV
                     RWTSITPASQGRLLFLAIGAISALIWMGWDAFVERVAVRFSNRPVSLVLKWCVPIVML
                     LVALRAPFADIAPTYAAVRYIERSDLPADLQTLNVTFDDRMRLLGIVPFRRVDSDGQL
                     RVTL"
     gene            complement(1755..4106)
                     /locus_tag="AC162G_03860"
     CDS             complement(1755..4106)
                     /locus_tag="AC162G_03860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR5074982.1"
                     /translation="MISASRFISIRIILAAYLLLGVTYSSVAPIFEASDELWHYPLVQ
                     WLSNGNPLPVQDPDNVGPWKQEASQPPLYYALVGWATSWIDTGDINDVLRPNPHVNNG
                     VITPDGNINLVVHDPAREAFPWRGTVLAVHLARLLSVLMGAATVWLTYRIALELFPPA
                     RGGDRGGAWFALSAAAVVAFTPMFLFISGAVNNDNLAMLLCTLALLLIVKRLRAPEEP
                     ATLGFRIGRWVPLGVVLGLATLSKTSAIALLPIAALTVSVVAWKRRSWPELIAGALGT
                     AVPLLLIAGWWYLRNIQLYGDVTGLNAFIEVLGRREAPASLAQLWGERWGFMLSYWGL
                     FGGVNVPMDDWVYHLLNALAWLALTGAVAYLISSTAKWFREDPGPPPSLCSGQALSLS
                     KGQPRRDFGRALSGYLQERMPLFAVGLFGVLVVAALTQWATVTWSSQGRLVFSAIAVW
                     SIFLVLGLTGLLPDSAGRCAGAALGVFLFTIAAIAPFATISPAYARPDPVTPPDTLDV
                     PLDVTFGGRIRLLGADVESDSARPGGSMEIILYWQASAPMDRDYSTFVHLLDENEIVV
                     AQRDMFPGQGLWPTSQMNVGHVIASHYVLNIPKTAYAPSALIWEVGVYDRSTPGEPRL
                     SVSSGGDNVRFGSIALAPRPGDVPNPAAINLGNQVELIGYDMDRRVAAPGETVRLTLY
                     WRARSRMSIDYTAFTHILEPPETLWAQHDKQPAPPTSTWVAGQVVSDTYELTIEPDTP
                     PGVYEVEVGVYYLRSPAFDRLRVLTDDGRITENYILLSKVRVR"
     gene            complement(4103..6076)
                     /locus_tag="AC162G_03865"
     CDS             complement(4103..6076)
                     /locus_tag="AC162G_03865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR5074983.1"
                     /translation="MTRSKQLVLVLTLLAFSLRAYRLDVQSLWSDEGLSLYRARLSLS
                     ENLSNVIVVPPGVSTQDTNPPLYFILLSGLCAAAGESEYALRFLSVMAGVLLVPLLYV
                     SARRLFSERAGVLSAALAALSPFLVWYSQEARMYTLLAALSTASVYLLLRALSLRGAS
                     RLQDEQERLRASRRAAWLVWAAVTAAGLYTHFVAFFVLLFECAVVLVVVAWRRRRDAL
                     LAAALVILAAVPATTYALSRAAVNPSPASGFRTLDSIVEELLGTFGIGWSNNFFQPLW
                     AVLPALGLLIVGAFGGRLQRPRHVFGGLFTLAWLLIVLMAFYVATFIQPLYTGPRHLL
                     LLALPFYLLVGNGLALAWRRSRLVGVASLGWMVVSMAVWLGVQFFDSTYTKQDIRSVA
                     MLIAKRAAAGDVVIVHDAVTSFVFDYYYDGAAPWAIIPTYPSHEVSAALAEFQARAEA
                     ADRLWFITEPPPLSGFDRQALDVWARGHLLRLDHQRFSALWLGSEYQLYTAHYPVLDA
                     LPGGAKPMDVTWDTGLRLVGVADVTGQLPPPTAAGSSHLALFWRQDRRAQGNYVTTFR
                     LVDPSGDERLRQRGTIFDNWSARQWPVGKIVRQDVTFNLPPDLPPGVYDVWLAVAERA
                     SEEVILTVDGEGDVAIGSLALPGEPVNGKLTNP"
     gene            complement(6080..>8276)
                     /locus_tag="AC162G_03870"
     CDS             complement(6080..>8276)
                     /locus_tag="AC162G_03870"
                     /inference="COORDINATES: protein motif:HMM:NF021436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HIR5074984.1"
                     /translation="DIRIIVMGFVGLGMAYGLATPIFEKPDEHWHMAYIASLLEARGY
                     PAPPVVIGDLTPAQESSQPPLYYTLAAGVARAAVSDLSDRADWLVHNPGFPYIAEETL
                     NDNKNRLMHALPESPTYGGTPLAVFVARMVALAMGAVAVGAAYGLGREAFPERHLVAL
                     TAAATVAFLPQFLFISSSVSNDSTAAATCGLAMWATTRILRRGMTSRRAASAGLALTL
                     AALSKASAVALMPLTLLVLAANLRRPYRDSLGQLTLVAAVPLLIAGPWYLRTWSLFGD
                     PLGASTHLAMTWAYAEPLSLIGTIGQLPGAAVSFWLAFGWGNVLLPDALYSVLNALAF
                     LGLAGAVIAGVRNRDRVTRLSLAYAATWIGLVFVALLMWIRVLSAPLGRLLYPALPAL
                     ALLLAAGWATLASPRRMWLAALPGVCLLLVSIAALPFVLMPAYARPALFSVEQLAAQP
                     GLATDVRFDDVARLVRVDVPRDVWPRPGQEPIVRLCWEALQSDARPLMVLVQFVGEAN
                     RVVATRRTLPGLGAFPTAAWRPGDRFCDAVRVPIPQDAPAPLMVRVEVSIIDPATRTR
                     LPAFAPDGLPLEATFVDRMKIAPAAYAEPPIEAAMNTRFGDQIALVGYAVEASTVRPG
                     EAVSFRLYWHALRRPQADYTVFVHLRDESGETVAQGDGPPQFGEYPASFWEAGEVVTD
                     ERVIAVPADTAPGDYALVVGLYELASGDRLLIDDETSQTEVTIGWVTVQ"
BASE COUNT         1785 a         2685 c         2560 g         1246 t
ORIGIN      
        1 gatcaagttg atgtgcgccc gcaccagggt ggggcgctgc gcatcagggt cgagtggaat
       61 gcgatactcg tcggcgaaga tcgcgcccgc cggacaatca ctcgtcgcca gcaagccacg
      121 atgcggataa gcgtcgatct tgccgatttc ggcaagatca cgccctttcg ccgaaattga
      181 cacgctgtaa ttgtcctgaa tggggacgag gcactgccac tacagcgtga ccctgagctg
      241 accgtccgag tcaactctcc gaaagggtac gattccgagc aatctcatcc ggtcatcgaa
      301 cgtcacgttc agcgtctgca ggtcggcggg caaatcagag cgctcgatat agcgcacggc
      361 cgcgtacgtc ggagcgatat cggcaaacgg cgcgcgtagc gcaaccaaga gcatcacgat
      421 aggcacacac cacttcaaga cgagcgacac aggcctattg gagaagcgaa cggcgacgcg
      481 ctccacaaac gcgtcccagc ccatccaaat cagcgcgctg atcgcgccga tggccaggaa
      541 gagcagccgt ccctgggagg ccggtgtgat cgacgtccag cggacgatgc cgacgaaaac
      601 cgtgagcaca tagagcgcca acaccagcca cggccacagg cgagatcggt ctccctcgcg
      661 ccagacgcga cgaacgtata acgcggcgcc cagtgccgcc agcaccgtca acatgtcgta
      721 gaagacgtat gccagaggca gggtcaggat attgactgcg ccaaacagcg cccagtaggc
      781 gatcttgaac ccctcgagct cgggtatcag ttgcagcagg gtcatggatc gacgcccggc
      841 gacgtcggcc attgtctcga tgccgagcca ctcgccgtac agctcgtagt tgcgcgcgta
      901 ccaccagccg gcgatcagcg ccacaccggc cgctagcatc agtcccgaac ccacccagcg
      961 tgtccactcg cgcttcggaa gatgaactac cgacagagta atcgccgccg gcacgagcag
     1021 agttaagcca ctcactttgg agagcgcggc caggccgatg gtcattgcca atgcggcacg
     1081 ccggatcagc cagcggcgct ggtccggctc cgcctcgcgc gatccatagc gtcgctcgtt
     1141 caggatgcgc atcgtcaata cgagcgcgat cgtcgagacc aggatcaaca agttgtcatt
     1201 gttgaccgaa gcggtgatga acaggaacat cggcgtgaac gccgcgatcg ccatcgcgcc
     1261 tgtcgccagg tcaggacgtc ccggagctat ctcacgcatc agcacaccga cgagcactac
     1321 tgtggtcgtg cccatcaaga tcgatacaaa gcggatgagg tgaacggcga gcgtcatacc
     1381 ttgccagggg gcattctcgg cgtcggagtg cacgacaaga ttcctgttcg catacagata
     1441 cggatcgcct ggctgcgcgt gcgggttgta gcgcaggcgc tcgctcacgt cgctcgtgtc
     1501 gatccagcgg gctatgggtg agaccaaaag gtaatacaac ggcggctggc tgccctcctg
     1561 ctcccaggct gtcttgacgc cggggcgctg caccggcagc cccagaccgt cggccatgtg
     1621 ctttacgacc gcgaaatgcg acagctcgtc agacgcttcg aagatgggca tgacgacgct
     1681 gtaaaggcag ctcaacagga caaacgcgac gacgaccagg cgctcaggcc gaatacttct
     1741 ccggtgagtc ggcttcatcg gacgcgcacc ttgctcaaca agatgtagtt ctccgtgatc
     1801 cggccatcgt cggtcagcac tcgcaggcga tcgaaagccg gcgatctaag atagtaaaca
     1861 ccgacctcga cttcatacac accgggtggt gtatccggct cgatcgtcaa ctcgtacgtg
     1921 tcgctgacca cctgccccgc gacccacgtg gaggtgggcg gcgcgggttg tttatcgtgc
     1981 tgcgcccaca acgtctcggg aggctcaaga atatgcgtga aggctgtgta gtcgatcgac
     2041 attctggaac gcgctcgcca gtaaagcgtc aggcggaccg tttcaccggg cgcagcgaca
     2101 cgccggtcca tatcatagcc gatcagctcg acttggtttc cgagattgat ggcggccggg
     2161 ttgggaacat cacccggcct cggcgcgagc gcaatcgagc cgaatcgcac attatcgccg
     2221 ccgctcgaca cagacaagcg cggctcgccc ggggtcgatc tgtcgtacac gccgacttcc
     2281 cagatgagcg cgctgggggc gtaagccgtc ttcggaatgt tgagcacata gtgcgaagcg
     2341 atcacatgcc cgacgttcat ctgcgaggtc ggccacaatc cctggccggg aaacatatcg
     2401 cgctgcgcca cgacgatttc gttttcgtcg agcagatgga cgaatgtgct gtaatcgcga
     2461 tccatcggcg cgctggcctg ccagtataga atgatctcca ttgacccgcc tggcctggcg
     2521 gagtccgact cgacatccgc gccgagcagc ctgatgcgac cgccgaacgt gacgtcgagc
     2581 ggaacgtcga gcgtgtcagg tggagtcacc ggatcagggc gggcataggc gggagaaatc
     2641 gtcgcgaacg gtgcgatcgc ggcgatggtg aacagaaaca cgcccagggc agcgccggca
     2701 catcgcccgg cactgtccgg cagcaaaccg gtcaagccca gcacgaggaa tatcgaccac
     2761 acggcaatcg cactgaagac caggcgccct tgcgatgacc atgtcaccgt cgcccactgc
     2821 gtcaacgcag cgacgacgag gacgccaaac aggcccacgg cgaacaaggg catgcgctct
     2881 tgtaggtagc cgctcaaggc gcgaccgaaa tcgcgacgcg gttggccctt cgacaggctc
     2941 agagcctgcc ctgagcatag cgaaggggga ggccctggat cctcgcgaaa ccacttcgcg
     3001 gtgctcgaga tgagatacgc aacagcgccc gtgagggcca gccacgctaa cgcgttgagg
     3061 aggtgataaa cccagtcgtc catcggcacg ttgacgccgc cgaacaatcc ccagtacgac
     3121 agcatgaatc cccagcgctc gccccacaac tgcgcgagcg aagcgggcgc ctctcgcctg
     3181 ccgagcacct cgatgaacgc attgaggccg gtcacatcgc cgtacagttg gatgttgcgc
     3241 agataccacc agccggcaat gagcaaaagt ggcacggccg tccccagagc gccggcgatg
     3301 agctcgggcc acgaccggcg cttccacgcg acgacggaca ccgtcagcgc ggcgatcggc
     3361 agcagcgcga tggcgcttgt cttggacaac gtggcaagcc ccagcacgac gccgagcggc
     3421 acccatcgcc cgatccgaaa accgagggta gctggctctt ccggcgcgcg cagccgcttg
     3481 acgatgagga gcagggcgag cgtgcacagc agcatggcca ggttatcatt attgactgcg
     3541 ccactgatga agagaaacat cggtgtgaac gcgacgaccg ctgccgcact caatgcgaac
     3601 caagcccccc ctctatcccc cccgcgagca ggcgggaaga gttcgagtgc aatgcgatag
     3661 gtcagccata ccgtcgccgc gcccatcaat acggacagca gccgcgcaag gtgaacagcc
     3721 aacaccgtcc cacgccaggg gaatgcttcg cgtgccgggt cgtgaacgac gaggttgatg
     3781 ttgccatcgg gcgtaatcac accgttgttg acatgcggat tcgggcgcaa cacgtcgttt
     3841 atgtcgcctg tgtcgatcca tgaggtcgcc cagccgacga gagcgtagta cagcggcggc
     3901 tggctggctt cctgcttcca gggccccacg ttgtcagggt cttgcaccgg cagcggattg
     3961 ccattcgaca gccactgcac cagcggataa tgccagagtt cgtcggacgc ctcgaagatc
     4021 ggtgccacgc tgctgtacgt gacgccgagt aggaggtaag cggcgaggat gattcggatg
     4081 gagatgaagc gtgatgcgct tatcacggat tcgtcaattt gccattgacc ggctcgccgg
     4141 gtaaggccag cgagccgatc gcgacgtcgc cctcaccatc cacggtcagg attacctcct
     4201 ccgacgcgcg ttcagcgacg gccaaccaga catcataaac accgggcggc agatcaggcg
     4261 gcaggttgaa ggtcacatcc tgccggacga tcttccctac cggccattgc cgagccgacc
     4321 agttgtcgaa gatcgtgccg cgctgccgca gacgctcgtc gccggatgga tcgaccaggc
     4381 gaaacgttgt gacatagttg ccttgcgcac gccggtcttg acgccagaag agcgctagat
     4441 gcgacgaccc ggccgccgtc gggggaggca attgacctgt gacatccgcc acgcccacca
     4501 ggcgcaagcc ggtgtcccag gtgacgtcca tgggcttggc tccacccggc agcgcgtcga
     4561 gcaccgggta atgcgccgtg tacaactgat actcagagcc aagccagagc gcggaaaaac
     4621 gctgatggtc gagccgcagc aggtggccgc gcgcccacac atccagcgcc tgcctgtcga
     4681 agccgctcag cggcggcggc tcggtgatga accacaggcg atcggctgct tctgctcgtg
     4741 cctggaactc ggccaaggcg gcactcactt catgcgatgg ataggtcggg atgatcgccc
     4801 agggcgcggc gccgtcgtag tagtagtcga atacgaatga ggtgacggcg tcgtgaacga
     4861 tcaccacatc tcctgccgcg gctcgcttcg cgatcaacat ggcaaccgat cgaatgtcct
     4921 gcttggtgta agtcgaatcg aagaactgca ccccgagcca cactgccatc gacacgacca
     4981 tccaacctaa gctggccaca ccgaccaggc gagatcgacg ccaggctagc gccagcccat
     5041 tgccgaccaa aagatagaat ggcaaggcca gcaaaagcaa atgccgtggc cccgtgtaca
     5101 gcggttggat aaacgtcgcg acgtagaagg ccatcagcac gatgagcagc caggccagtg
     5161 tgaacaatcc gccgaacacg tgccgtggcc gttggagccg cccaccgaac gcgccgacaa
     5221 tcagtagtcc aagcgcaggc aagacggccc acaacggctg gaagaaattg ttagaccagc
     5281 cgatgccgaa cgtgcccagc agctcctcga cgatgctgtc cagtgtgcgg aaacccgatg
     5341 ccggcgacgg gttgaccgcc gcgcgcgaca gcgcatacgt cgtggccgga accgccgcaa
     5401 gaatgacaag cgccgcggcc agaagcgcgt cacgtcgccg gcgccatgcc acgacgacga
     5461 gcaccacggc gcattcgaac agcaacacga agaaggcgac gaagtgcgta tataggccgg
     5521 ccgccgtgac agccgcccat accaaccaag ccgcgcgccg cgaagcgcgg aggcgctcct
     5581 gttcatcctg cagccgtgac gcgccgcgca gcgacagagc acgcagcagc agataaaccg
     5641 aggccgtcga tagcgcagct agcagggtgt acatacgcgc ttcttgcgaa taccacacca
     5701 ggaacggtga cagcgcagcc aaggccgccg acagcacacc cgcacgctcc gagaagagtc
     5761 gcctggccga cacgtacaac aacggcacca ggagcacgcc cgccatcacg gacagaaagc
     5821 gcagcgcgta ctcgctctcg ccagcggcgg cacatagtcc gctcagcaga atgaagtaca
     5881 gcggcggatt ggtgtcttgt gtcgacacgc cgggcggcac gacgatgacg ttgctcaggt
     5941 tctcgctcag cgaaaggcgc gcgcgataca gcgacagtcc ttcgtcgctc cacaggctct
     6001 gcacatccag ccggtaagcg cgcagcgaaa acgcgagcag cgtcaacacc agaacgagct
     6061 gcttggaccg ggtcatttgc tactgtaccg ttacccaacc gatcgtgact tccgtctgcg
     6121 acgtttcatc gtcgatcagc aggcgatcgc cgctggcgag ttcgtatagc cccacgacga
     6181 gcgcgtagtc gccaggtgca gtgtccgccg gcactgcaat gacgcgttcg tcggtgacga
     6241 cctcgccagc ttcccagaac gaagccggat attcgccaaa ctgcggcggg ccgtcaccct
     6301 gagccaccgt ttcgcctgac tcatcgcgta gatggacgaa gaccgtgtag tcggcctggg
     6361 gccgccgcag cgcgtgccaa tacaaccgaa atgagacggc ctccccggga cggaccgtcg
     6421 aggcctcaac cgcgtagccc accagcgcga tttgatcgcc gaaccgggta ttcatcgcgg
     6481 cttcgatggg cggctcggcg tatgccgcag gcgcgatctt catccggtcg acgaacgtcg
     6541 cttcgagcgg caagccgtct ggcgcgaagg cggggagccg cgttctggta gcgggatcga
     6601 tgatcgacac ctctacccgg accatcagcg gtgccggcgc atcctgcggg atcggcacgc
     6661 gcaccgcgtc gcagaagcga tcgccgggcc gccaggcggc tgtcggaaac gcgccgagac
     6721 cgggcagcgt gcgtcgtgtg gcgacgacac ggttggcctc gccgacgaat tgcaccagca
     6781 ccatcagtgg acgagcgtcg gattgcagcg cttcccagca cagacgcacg ataggctcct
     6841 ggcccggacg cggccacacg tcacggggca cgtcgacgcg aaccaggcgc gccacgtcat
     6901 cgaagcgaac atccgtcgcg agaccgggtt gtgcagccaa ttgctcgacc gagaacaagg
     6961 cagggcgagc ataggccggc atgaggacga agggcagcgc ggctatgctc acgagcagca
     7021 ggcacacccc gggcagcgcc gcgagccaca tgcggcgcgg cgaagccagc gtcgcccagc
     7081 ccgccgcgag cagcaaggcc aatgccggca gcgccggata tagcagccga cctaacgggg
     7141 cactcagcac gcgaatccac atcagcaatg cgacgaagac cagcccgatc caagtcgcgg
     7201 catacgcgag gctcaatcgc gtgacgcggt ctcggttgcg cacgccggcg atgaccgcgc
     7261 cagccaagcc gagaaacgcc aacgcattga gaaccgaata cagcgcgtcg ggcagcagca
     7321 cattgcccca gccaaatgca agccagaacg acacggccgc ccccggcaac tgaccgatcg
     7381 tgccaatcaa cgacaacggc tcggcatacg cccatgtcat ggctaggtgc gttgatgcgc
     7441 cgagtggatc gccaaatagc gaccaagttc tcaaatacca cgggccggca atcaacagcg
     7501 ggactgccgc aaccagtgtt agctgtccca gagagtcgcg gtaagggcga cgcaggtttg
     7561 cggccaggac cagtagcgtc agcggcatca acgccactgc gctggccttg ctcagcgcgg
     7621 ccagtgtgag cgcaagccct gcgctggcgg cgcgccggct cgtcataccc cgccgcaaga
     7681 tgcgcgtcgt cgcccacatc gccagcccac aagtcgccgc cgccgtgctg tcgttgctga
     7741 cagaagaact gataaatagg aattgcggaa ggaaagcgac cgtggccgcg gctgtcagcg
     7801 ccaccaggtg tcgctcggga aatgcctcac ggccaagacc gtaggcggca ccgaccgcta
     7861 cggcccccat agccagcgca accatccgcg caacgaagac ggccagcggg gttccgccat
     7921 aggtgggcga ctcgggcagg gcgtgcatga gtcggttctt gttgtcattc agcgtttcct
     7981 ccgcgatgta cggaaaaccg gggttgtgca caagccagtc cgctcggtca ctcaaatcgg
     8041 aaacagccgc acgcgccaca ccggccgcca aggtgtaata cagaggcggc tggctgctct
     8101 cctgggccgg cgtgagatcg cctatcacga ccggcggcgc aggatagcca cgagcttcca
     8161 gtagcgaggc gatatacgcc atgtgccagt gctcgtcagg tttctcgaag atgggcgtag
     8221 ccagtccgta ggccatgccc agcccgacga agcccatgac gatgattcgg atgtcg
//
LOCUS       DBNHXB010000102        15933 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3876964, whole genome shotgun sequence.
ACCESSION   DBNHXB010000102 DBNHXB010000000
VERSION     DBNHXB010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 15933)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15933)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15933
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3876964"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="AC162G_03875"
     CDS             complement(<1..323)
                     /locus_tag="AC162G_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HIR5074985.1"
                     /translation="MTDRLSVVIRGGGDLATGVALRLHRAGFRITVLEIPQPTVIRRA
                     VAFASAVYDGSTVVEGVTARLMGLDDDLEPIWRSGEVPVVVDPDASILARLRPDALAD
                     AIMAKR"
     gene            complement(316..1647)
                     /locus_tag="AC162G_03880"
     CDS             complement(316..1647)
                     /locus_tag="AC162G_03880"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5074986.1"
                     /translation="MSSTTPSASTAALPHALTPNERRSFVLGVSNGAAFTFAESLIAA
                     DLVLTNFVGKLTASNFMIGLVVPLRDVGWFLPQLFVSSYLQYRERVLPIYRAMAVVRT
                     IAWFGMGAVVVAVRDPGALLLLFFLLYGVNSLAAGMSGLSFMDVVAKTIPAGRRGSFF
                     AGRLFFGSLLGLLASVIVSAALGGTLGLSYFDSIGVLFLVSWAAASLGLLAFGAIREP
                     PGEIREEPSTLGSHVRRAARLPRDNREFRLLLVGRVMLILGYVAAPFYGVYATRELGA
                     DARILGVFLAARTLSFLVANPAWARLSDRRGNRLVMLIALAFGLLMPSVALAAKPVMS
                     LLAVPPAQWALFYVSVFLLWGLFESGIGVGAANILLDFAPPRDRVTYVGLTNSVLGVA
                     LLSTSLGGALVDLIGISSVFLLSLGCLAASLIAFYKMREPREPSPVDEAHD"
     gene            complement(1670..2719)
                     /locus_tag="AC162G_03885"
     CDS             complement(1670..2719)
                     /locus_tag="AC162G_03885"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5074987.1"
                     /translation="MHRERIADDVYVFTSDLYAQVTAGVVVTSEGAVVIDTLPFPSET
                     RKLLEFLSERSPLGIRYLILTHYHADHTLGASLFRGVPIISHALCRDLLAERGAAALA
                     SARAQNPELSEVSLRLPDIVFDGGELSVRLGNKTIALAHLPGHTDDCLAAYVKDDKIL
                     FASDVMTPVPMIVDGDPRRLIESLRRIKSFGLESIVQGHGEMILRGEINESVNSNISY
                     LNRIISLVASLQRDRRPRESVREYGIEACGKSRIPLHGLVQQFHAANLHALYNRMALD
                     KSLYRLGLRLLVERETAKVDGGKAKPSKRATPAAQPKKKPVSRASKAGPGAEKSVARS
                     RKTTRASVSAKAKKK"
     gene            complement(2826..3003)
                     /gene="ssrS"
                     /locus_tag="AC162G_03890"
     ncRNA           complement(2826..3003)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC162G_03890"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3109..4869)
                     /gene="aspS"
                     /locus_tag="AC162G_03895"
     CDS             complement(3109..4869)
                     /gene="aspS"
                     /locus_tag="AC162G_03895"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR5074988.1"
                     /translation="MYRTHTCGELRATHVGRSATLAGWLHKRRDHGGLIFIDLRDRYG
                     ITQVVVDPSQAGAYAAAGKARGEFVLRVEGTVRARPEGLANPNLATGEIEVAASSIEI
                     LSAAKPLPLPVDDDGYKVDESLRLKYRYLDLRRARMQSNLVLRHKVVRFIRDYLDTQG
                     FVEVETPIMIKSTPEGARDYVVPSRLQPGRFYALPQSPQQLKQLLMVAGYDRYFQIAR
                     CFRDEDLRGDRQPEFTQLDLEMSFVERGDVLAVVEGLITEMIPAVAPRKRVLSPFPQL
                     PCREALARYGSDKPDLRFGMELVDLSEALAESDFNVFRSTVASGGVVKAICARGCAGY
                     SRKEVDALTALAQANSAKGLATLAWTGDGVKGTLAKFLKEGEIAKIAGRLGAGEGDLA
                     LIVADQEPVANKALGALRLELRDRLNLADPDLLAFGWVIDFPMFEWNAEENRWDVAHH
                     PFTMPKAEHLDRLESDPASVLSDAYDLVCNGYELSSGSIRIHRRDIQNRVFRLLGYSE
                     EETQRRFGHMLEAFEFGAPPHGGMAPGIDRLVMLLADEPNIREVIPFPKTASGSDLMT
                     DAPSPVDARQLRELRLKIVE"
     gene            5247..5900
                     /locus_tag="AC162G_03900"
     CDS             5247..5900
                     /locus_tag="AC162G_03900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5074989.1"
                     /translation="MKTRTNAEWLQELESTGDAQAAAIEDLRAYLLRAALYSLSRSHD
                     DLAHLAPAEIEHVAEDCAQDALLAILKHLREFRGDSQFTTWAYKFAVNIALVAARREG
                     WKRVSLDQLLDHPDLPEWPIRDEHVSVDPDRRTLQGEVWATMREVIRDDLTERQRQVL
                     RAMVFDEVPMDEIVRHLGSNRNAIYKLLHDARRKLKAQLEARGFDVQEILNLFSAKT"
     gene            5944..6261
                     /locus_tag="AC162G_03905"
     CDS             5944..6261
                     /locus_tag="AC162G_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074990.1"
                     /translation="MNRSKFERWVRQIQETQEEEVSCSDCFDRVSQYVDSVNAGGAVA
                     GPMPCLKPDQQPCPVCRELPCLRQHLLQCRVCREEYETLRDLARLEAEGRAPSVDDLL
                     KSL"
     gene            6440..7639
                     /gene="tal"
                     /locus_tag="AC162G_03910"
     CDS             6440..7639
                     /gene="tal"
                     /locus_tag="AC162G_03910"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HIR5074991.1"
                     /translation="MTDNRVKELKRLGQSIWYDNISRELITSGRLRELIDEGLWGMTS
                     NPTIFEQAILGSHGYDDSMRTLIENATDVGEIYDALTLEDVGMAADEFRRLYDETDGT
                     DGFVSIEIAPTLARRTEDTIKEARRLWTALDRPNIMVKVPATRAGLPAIEQLLREGIN
                     INITLMFSMAHYEAVAEAYLRGLEARAAPGEPVDHVASIASFFVSRVDAMVDRMLEAI
                     VRREGPEAGLAASLIGKAAIANSKLVYERFRQIFEGQRFAALKVRGAQLQRVLWASTS
                     TKNPNYPDTLYVDTLIGPHTVNTVPPHTYAAIQDHTIVRRTVDQDLDSAREVVSGLAR
                     LGIDLNEVGERLSDEGVDKFTKSYEQVMDAIVAKRDELMPAVVAPQKSSARKTAVKKK
                     TTTRRTK"
     gene            7660..8025
                     /locus_tag="AC162G_03915"
     CDS             7660..8025
                     /locus_tag="AC162G_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074992.1"
                     /translation="MQIRIEIVPHASGGLEAKVTQVHDDQVVMDYLEAVVGEIARGRP
                     VVVTRTARQETVTVAVQLPVGKRGGPAGTPEDQKMEIVKGWLEVRGRMNQEVYAHSRA
                     VASSTLRRWMRELREAGKL"
     gene            8111..9442
                     /locus_tag="AC162G_03920"
     CDS             8111..9442
                     /locus_tag="AC162G_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074993.1"
                     /translation="MKTRFGVFAALASFILIGVAATAFAHNQADLNGQAFDHLINQNA
                     RRMVREGRQIFRFDTFGDEVFWGDTLRLHEAIAGEALGGVGPGVSPRTALAVGLKVDV
                     DALPRDLVRKLRSGEVDLDDPATTLALLKLNAVVGVTGFFDEDGNLQSMGIQCALCHS
                     TVDDSFAPGIGRRLDGWANRDLNVGAIVALAPNLTPLADLLGVNEETVRAVLNSWGPG
                     KFDAEIILDGKAFQPDGRSAATLLPPAFGLAGVNLHTWTGWGSVTHWNAFVANLEMHG
                     QGTFFDPRLNDPTQFPIAAQAGFGNVRNTPDLITSKLAALHFYQLAIPAPAPPEDSFD
                     EESAARGEVSFNGKANCASCHVPPLFTEPGWNMHTPAEIGIDDFQANRAPDRHYRTSP
                     LQGLWTHMKGGFYHDGRFATLRDVVDHYNAFFNLGLTEEEINDLIEYLKSL"
     gene            9884..10603
                     /locus_tag="AC162G_03925"
     CDS             9884..10603
                     /locus_tag="AC162G_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074994.1"
                     /translation="MNTIRFKIAHIAAAVLVSISAFAALAAVNDYASAASSAVVAGEV
                     SRHDHLDFGPRDPLPLKVLAFHDAMRKLWEDHITWTRMFIVSALADLPDVSHAAERLL
                     QNQADIGDAVKPFYGEAAGEQLTALLRDHILIAADLVAAAKAGDAAGVEEASARWYAN
                     ADEIAAFLNSANPDNWPLDEMKAMMREHLDLTLQEAVARLTGNWAADIAAYDEIHEQI
                     LGMADMLSSGIIRQFPKQFFK"
     gene            10717..10872
                     /locus_tag="AC162G_03930"
     CDS             10717..10872
                     /locus_tag="AC162G_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5074995.1"
                     /translation="MGQLYGYFQPAVVFDGKHHSVAPVVVDASLPYNIIHVLNRCGAV
                     IGLKDQP"
     gene            10885..12069
                     /locus_tag="AC162G_03935"
     CDS             10885..12069
                     /locus_tag="AC162G_03935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5074996.1"
                     /translation="MPDYMYLIIGGGMTAEAAVRGIRALDSSGSIGLIGAEPEPPYNR
                     PPLSKGLWKGKSIDRIWRKTEGQGVELHLGRTAQRLDPRNKRVTDDQGTLYTFDKLLL
                     ATGGTPRHLPFSSDQIIYYRTVEDYRRLRALAEQRQRFAVIGGGFIGSEIAAALAMNG
                     KDVVMMFPSKGIGARVFPRDLSLFLNDYYRQKGVEVLVGEAVVDLEPRADRVALKTRS
                     GREIVVDGVVAGIGIQPNVELAQAAGLDVEDGIVVDELLRTSHPDVYAAGDVAFFYNP
                     ILGKRLRVEHENNANAMGRMAGRVMAGEVTPYHHLPFFYSDLFELGYEAVGDVDATLE
                     AVADWKEPHHEGVVYYLQAGRVRGVLLWNVQKQVDAARRLVAEPGPFRPADLKGRLPE
                     AH"
     gene            12217..13089
                     /gene="nadC"
                     /locus_tag="AC162G_03940"
     CDS             12217..13089
                     /gene="nadC"
                     /locus_tag="AC162G_03940"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR5074997.1"
                     /translation="MKELAALQLDEILDQVAPAVRRALEEDIGDGDATTLFTIPTDAT
                     LGGRFVAKEPGVIAGLSVAQLTFRLVDERVLFTPHVADGDSVQQGQAFAVVSGPCRAL
                     LTAERVALNFLQRMSGVATLTRRFVQAVQGTRAVILDTRKTAPGLRLLDKWAVRIGGG
                     QNHRFGLYDMVLIKDNHIAAVGSISQAVARVRAADDRRRPIEVEVKTLAELEEALPLD
                     VDRILLDNMSRDEMRQAVRITNGRTPLEASGNVTLENVAEVAATGVDYISAGALTHSV
                     RALDISLQLDPIVR"
     gene            13411..14463
                     /gene="nadA"
                     /locus_tag="AC162G_03945"
     CDS             13411..14463
                     /gene="nadA"
                     /locus_tag="AC162G_03945"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR5074998.1"
                     /translation="MSAEALYEQMKAKLHDVVPDFELRHKAVIADEINRLKAEKNAVI
                     LGHNYMEPALFHSIPDYTGDSLELSRIAAKTDKDIIVFCGVKFMAETAKLLNPTKMVL
                     LPSTKAGCSLAASITAEDVRQLRKRFPGVPVVTYVNTYADVKAESDICCTSSNAKAVV
                     ESLGTDTVIFLPDEYLARNVARETGKHIIFPTLHADPGASTELDYQMIGWHGRCEVHE
                     KFTVQDIRSVRQQFPDVMILAHPECSPEVVAASDYSGSTTAMIKHVQKTNAPRYLLLT
                     ECAMGDNIMAANPDKEMLRLCSVRCPHMNQITLEDTLDSLKLNQYVIDVPEDVRVRAA
                     QAVERMVAIGAIGGRD"
     gene            14483..>15933
                     /gene="nadB"
                     /locus_tag="AC162G_03950"
     CDS             14483..>15933
                     /gene="nadB"
                     /locus_tag="AC162G_03950"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HIR5074999.1"
                     /translation="MSEIVETEVLIIGCGIAGGVAALQLADAGIPVAVVTRAQEPSES
                     NTYYAQGGIIYRGADDSPALLAEDVLRAGAGHSNPRAVTILAEEGPPLVRQTLIERIG
                     VQFDRNAGGDLSLALEGGHSVPRIVHAADATGRAIEVALLEALRAHKNVTLLTGHTAI
                     DLLTPSHHALNRLAVYEPQSCVGAYVLDQAADRVIRCLARYTILATGGLGQIFLCTTN
                     PPGARGDGVAMAYRAGARVINMEFVQFHPTTFYHPQAARFLISEAVRGEGARLVHANG
                     EPFMQNYDAEWRDLAPRDVVARSIYREMLMRDVPNVYLDLRSTISEQRIREHFPNIYQ
                     ACLAYDVDITRDLAPVVPGAHYACGGVWVDEWGCTTLDRLYAVGEVACTGAHGANRLA
                     STSLLEGLLWGYRTARHILEHRAEREMPNTADIPAWRDDGLKPPDPALIQQDMSTIKQ
                     MMWNYVGLVRTTRRLERALSELRHLETEIEQFYR"
BASE COUNT         3222 a         4781 c         5066 g         2864 t
ORIGIN      
        1 cgcttcgcca taatcgcatc ggcgagcgcg tcaggccgaa gccgcgccag gatcgacgca
       61 tcgggatcga cgacgaccgg cacttcgccg gagcgccaga tcggttcgag atcgtcgtcc
      121 agtcccatga gccgggcggt gacgccttcg acgacggtcg atccgtcgta caccgccgat
      181 gcgaaggcga cggctctgcg gatcacggtc ggctgaggaa tttcgagcac ggtgattcgg
      241 aagccggcgc gatgcaggcg cagcgcgacg ccggtcgcca gatcgccgcc gccgcgtatg
      301 acgacactca atcgatcagt catgcgcctc gtccaccgga gacggctcgc gcggctcgcg
      361 cattttgtag aaggcgatca ggctggcagc caggcacccc agggagagca ggaacacgct
      421 ggagatgccg atcagatcga cgagcgcgcc gcccagcgac gtgctcagca gcgcgacgcc
      481 gaggacgctg ttcgtcagcc cgacgtacgt gacgcgatcg cgcggcggcg cgaagtcgag
      541 caggatgttg gccgcgccga cgccgatgcc ggactcgaac aatccccaca gaagaaacac
      601 cgacacgtag aaaagcgccc attgcgcggg gggcactgct agaagagaca tgaccggctt
      661 tgccgccagg gcgacagacg gcatgagcaa gccgaaggcg agcgctatca gcatgaccag
      721 ccggttgccg cgccggtcgc tgaggcgcgc ccaggccgga ttggcgacga ggaacgagag
      781 cgtgcgcgca gccagaaaca cgcccaggat gcgcgcgtcc gcgcccagct cgcgcgtcgc
      841 gtacacgccg taaaacggcg cggccacgta gcccagtatc agcatcacgc gcccgacgag
      901 cagcaggcgg aattcgcgat tgtcgcgcgg cagccgcgcg gcgcggcgca cgtgcgagcc
      961 gagcgtgcta ggctcttcgc ggatctcgcc cggcggctcg cggattgcac cgaatgccag
     1021 aaggccgagc gaggccgccg cccacgagac gaggaacagc acgccgatcg aatcgaagta
     1081 agacaggccc agcgtcccgc cgagcgcggc gctgacgatg acgctggcaa gcaggccgag
     1141 caggctgccg aagaacaggc gcccggcgaa gaacgaccca cgccgcccgg cgggaatcgt
     1201 cttggccacg acgtccatga acgagaggcc cgacatgccg gcggcgaggc tgttgacgcc
     1261 gtagagcaga aagaacagca ggaggagcgc acccgggtcc cggacggcga caaccactgc
     1321 gcccatcccg aaccaggcta tcgtgcgcac gacagccatg gcgcggtaga tcggcagcac
     1381 gcgctcgcgg tactggagat acgaagagac gaacaactgt ggcaggaacc agccgacgtc
     1441 gcgcagcggc acgaccaggc cgatcataaa attggacgcc gtcagcttgc cgacgaaatt
     1501 cgtcagcacc agatcggcgg cgatgagcga ctcggcgaaa gtgaacgccg cgccgttgga
     1561 tacgcccagc acaaagctgc ggcgctcgtt gggagtcaag gcgtgcggca gggcagcggt
     1621 ggacgcagaa ggggtagtag atgacacggc ggcgtgtgga gaagtcgaat tacttcttct
     1681 tcgctttcgc agatactgat gcgcgcgtcg tcttacgact gcgcgcgacg ctcttctccg
     1741 ctcccggccc ggccttagaa gcgcgagaga cgggcttctt cttcggctgc gcggcgggcg
     1801 tcgccctctt cgatggcttg gccttcccgc catccacctt cgccgtctcc cgctcgacga
     1861 gcaggcgcag gcccaggcgg tagaggctct tgtctaacgc catgcggttg tacagcgcgt
     1921 gaagattcgc ggcgtggaat tgctgcacca gcccgtggag ggggatgcgg ctcttgccgc
     1981 acgcctcgat gccatactcg cgcacggact cgcgcggccg gcgatcccgc tgcagactcg
     2041 ccacgagcga tatgatgcga ttgagatagc tgatgttgct attgaccgac tcgttgatct
     2101 cgccgcgcaa gatcatctcg ccgtggcctt gcacgatgct ctccaggccg aaagacttga
     2161 tgcgccgcag tgactcgatc aagcggcgcg gatcgccgtc gacgatcatc ggcacgggcg
     2221 tcatcacgtc cgaggcgaac aggatcttgt cgtccttcac gtaggccgcc aggcagtcgt
     2281 ccgtatgccc ggggagatgc gccagcgcga tcgtcttgtt gcccaggcgc acggacagct
     2341 cgccgccgtc gaagacgatg tccggcaggc gcagggacac ctcggacagc tccgggttct
     2401 gtgcgcgcgc gctggccaga gccgctgcgc cgcgctcggc cagcaagtcc cggcatagcg
     2461 catgcgagat gatcggcacg cctctaaaca agctcgcccc cagcgtgtgg tcggcgtgat
     2521 aatgagtcag gatcaggtag cggatgccaa gcggcgagcg ctcgctcagg aactccagga
     2581 gctttcgtgt ctcggatggg aacggcagcg tgtcgatgac gaccgcgccc tccgaggtga
     2641 ccaccacgcc ggccgtgacc tgggcgtaca ggtcgcttgt aaaaacgtag acgtcgtcgg
     2701 cgattcgttc tcgatgcata gcactgctgc gcgatgagat ggaacgggcg taagattagc
     2761 acatttgaaa cggctgtgtc aaggcaagta aaaaggccac tcaatgtgag cggccttcat
     2821 catgaaatag ccccgccatg ccgtgtgccg cagagttttg aacctgctct cgcaggtggg
     2881 agcctgacct gtcagttctg ccttgcctca cggctaccgc gtccctgacg agagaaacgg
     2941 ctccctgtat tatgggtgtt ggctcaaaac gcgtgggcga catcacgcgc acggcagggt
     3001 tcgttttgca ctataccaca aatgccgcgt tcccgcaagc gatttttaca cgcgcccaaa
     3061 gatttttaag gcgcaatagg cggatggtca taacattagg tcaacccttc attcaacgat
     3121 cttcaaacgc agctcgcgca actggcgagc gtcgacgggc gacggcgcgt cagtcatcag
     3181 atcgctcccg ctggccgtct tggggaacgg tatgacctcg cgaatgttcg gctcgtcggc
     3241 cagcagcatc accagccggt cgatgccggg cgccatgccg ccgtgcggcg gcgcgccgaa
     3301 ttcgaacgcc tcgagcatgt gaccgaatcg ccgctgcgtc tcttcttcgg aatagcccaa
     3361 cagcctgaag acgcggtttt gtatgtcgcg ccgatggata cgaatgctgc cgctgcttag
     3421 ctcatacccg ttgcacacca gatcgtaggc atcgctcagg acggacgcgg gatcggactc
     3481 caggcgatcg aggtgctcgg ctttcggcat cgtgaacgga tggtgcgcga cgtcccagcg
     3541 attctcctcg gcgttccact cgaacatcgg gaagtcgatc acccagccga aggccaagag
     3601 gtcgggatcg gcaaggttca gccggtcgcg gagctcgagc cgaagcgcgc cgagcgcttt
     3661 attcgcgacg ggttcctgat cggcgacgat caaagccaga tcgccctccc ctgcccccag
     3721 gcggcccgca atcttggcga tttcgccctc tttcaggaat ttcgcgagcg tgcccttgac
     3781 gccgtcgccc gtccaggcca gcgtagcgag acctttcgcg ctgttcgctt gcgccagggc
     3841 cgtgagcgcg tcgacttctt tgcgcgaata tccggcgcat cctctagcgc agatcgcctt
     3901 gacgacgccg ccggacgcaa ccgtcgaacg gaatacattg aaatcgcttt cggccagcgc
     3961 ctcgctcaga tcgaccagct ccatgccgaa gcgcagatcg ggcttgtccg acccgtagcg
     4021 cgccagggct tcccggcagg gcagttgcgg aaagggtgac aggacgcgct tgcgcggcgc
     4081 gacggccggg atcatctccg tgatcaggcc ttccaccacg gccagcacgt cgccgcgctc
     4141 gacgaaggac atctcgaggt cgagctgggt gaactctggc tgccggtctc cgcgcaggtc
     4201 ttcgtcgcgg aagcagcgcg cgatctggaa atagcggtcg tatccggcga ccatcaacaa
     4261 ctgtttcaac tgctgcggcg actgcggcag cgcgtagaat ctgccgggct gcaggcggct
     4321 gggcacgacg tagtcgcgag cgccctccgg cgtgctcttg atcatgatcg gcgtctcgac
     4381 ctcgacgaaa ccctgcgtgt cgaggtagtc gcgaatgaat cttacgacct tgtgacgcaa
     4441 aacgagattc gactgcatac gcgcgcgccg caggtcaaga tagcgatact tcaggcgcaa
     4501 agactcgtcg accttgtagc cgtcgtcgtc gacgggcagt gggagtggct tggccgcact
     4561 gagtatttca atcgaggatg ccgccacctc gatctcgcct gtcgccaggt tcgggttcgc
     4621 cagaccttcg gggcgcgcgc gtactgtccc ctcgacgcgc agcacgaact cgccgcgcgc
     4681 cttgccggcg gccgcgtacg ccccagcctg cgacggatcg acgacgacct gcgtgatgcc
     4741 gtagcggtcg cgcagatcga tgaagatcag gccgccgtgg tcgcgccgct tgtgcagcca
     4801 gccggccagc gtggccgatc gacccacatg cgtggcgcgc agctctccgc aagtgtgcgt
     4861 ccggtacata ggtttgctcc tctcgaaaag gaaaccgccc tcgcgggtga gggcggcgct
     4921 cgcttcggtc tggccggtgt ttctggtcaa cgcacgcgac gaaacgatca acgccctcgg
     4981 gcattgccgt cattgttgtc gcgattgtcg accaccgaga cccgtttcag catggtggca
     5041 ttataaatcg attccgagaa acctgtcaat ctttgtcgcc gatacgcgtg aggtctgagg
     5101 cgtcgaccta ttccgttggc tggtagactt catgttagtc cacgccgggt tggctaaggc
     5161 agagaacgta tcataatgac actcgtcgag cggaaggcgt aaaggtcact gttgagtgta
     5221 taatgacacg gttccatatc cataccatga agacacgcac caatgccgaa tggttgcagg
     5281 agttggagtc gacgggcgat gcgcaagctg ccgcgatcga ggatttacgc gcttacctgc
     5341 tgcgggcggc gctgtattcg cttagccgta gccacgacga tctggcgcac ctggcacccg
     5401 ccgagatcga gcacgtcgcc gaagattgtg cgcaggatgc cttgttggca atcttgaagc
     5461 atctacgcga gtttcgcggc gatagccagt tcacgacgtg ggcctataag ttcgccgtca
     5521 acatcgccct cgtcgccgcg cggcgtgaag gctggaagcg cgtctcgctc gaccaattgc
     5581 ttgaccatcc tgacttgcct gaatggccga ttcgggacga acacgtatca gttgaccctg
     5641 atcgtaggac actgcaaggt gaagtctggg cgacgatgcg cgaggtgatc cgtgacgacc
     5701 tgacggagcg gcagcggcag gtgctgaggg cgatggtgtt cgacgaggta ccgatggacg
     5761 agatcgtccg ccacctgggg tcgaatcgca acgcgattta caagttactg cacgatgcgc
     5821 gccgcaaact caaagcgcag ctcgaagcgc gcggcttcga cgtgcaagag attctcaacc
     5881 tgtttagtgc gaagacgtaa ggttcgaggc acatctgcaa ctaataagac aggaggccta
     5941 ggtgtgaatc gctcgaagtt tgaacgttgg gtgcgacaga ttcaggaaac ccaggaagaa
     6001 gaggtctcgt gctcggattg cttcgaccgg gtttcccagt acgtggattc ggtaaatgcc
     6061 ggaggggcag tggcgggacc gatgccgtgc ctgaaaccgg accaacagcc ctgcccggta
     6121 tgtcgtgagt tgccgtgtct gagacagcac ctgctccaat gccgcgtctg ccgtgaagaa
     6181 tacgaaaccc tgcgcgacct ggcgcgcctg gaagcggaag gtcgcgcgcc atcagtcgac
     6241 gatctcctga aatcactctg atcctcaatc gacccctaga ccaggcgaca ctgtcctttc
     6301 gtacggtgtc gctttttgtt gggtaagaac cggcccgctt gtgaaacaca tggatagatg
     6361 tacccaataa gacttctgcg aggaggatca agcggaagct gccgcaccac tgtcaggcat
     6421 cgctcaaaag gagaatcaca tgacagataa tcgtgtgaag gaattgaaga ggctgggtca
     6481 gagcatctgg tacgacaaca tcagccgcga attaatcacg tcgggcaggc tgcgtgaact
     6541 gatagacgaa ggcctgtggg gcatgacctc caacccgacg atctttgagc aggcgatctt
     6601 gggcagccac ggctatgacg attcgatgcg gaccttgatc gaaaacgcta ctgacgtcgg
     6661 cgagatttac gatgccttga cgcttgagga tgtgggcatg gctgccgacg aattccgccg
     6721 cttgtatgac gaaacggatg ggaccgatgg tttcgtcagc attgaaatag cgccgacgct
     6781 agcgcgcagg actgaggaca cgataaagga agcgcgccgg ctgtggacag cgctcgaccg
     6841 gcccaacatc atggtcaagg tgccagctac gcgtgccggc ctgccggcca tcgagcagtt
     6901 gcttcgcgaa ggcatcaaca tcaacatcac gttgatgttc tcgatggccc attacgaagc
     6961 cgtggccgaa gcctacctcc gtggtctgga ggcgcgcgcg gcgccgggtg agccggtcga
     7021 tcatgtcgcc tcgattgcgt cgttcttcgt cagtcgcgtc gacgccatgg ttgataggat
     7081 gctcgaagcg atcgtccgcc gcgagggtcc ggaagcgggg ctggccgcgt cgctgatcgg
     7141 caaggcggca atcgccaact cgaagctggt ttacgaacgc ttccggcaga tcttcgaggg
     7201 tcaacgtttc gcggcgctca aggtgcgcgg cgcgcaattg cagcgcgtgt tatgggccag
     7261 caccagtacc aagaatccaa actatcccga tactctgtac gtcgatacgc tcatcggccc
     7321 ccataccgtg aacacagtcc cgccacacac ttatgcggct atccaagatc acaccattgt
     7381 gcggcgcacc gtcgatcagg atctggactc ggctcgtgag gtggtgtccg ggttagcgcg
     7441 gctggggatc gatctgaacg aggtcggcga gcggttgtcc gacgagggag ttgacaagtt
     7501 cacgaaatcg tatgagcaag tgatggatgc gatcgtcgcc aagcgtgacg agctaatgcc
     7561 ggccgtggtt gcgcctcaga aatcaagtgc ccgtaagact gctgtgaaga agaagacgac
     7621 cacccgccgc acgaagtaat actgctttaa gccaacatca tgcagatcag aattgagata
     7681 gtgcctcatg cctctggggg gttggaagcc aaggtgaccc aggtgcatga tgaccaggta
     7741 gttatggact acctcgaagc cgtggtggga gagatcgcgc ggggtcggcc agtggttgtc
     7801 acgaggacag cccggcagga gacggtaact gttgccgtgc agttgcctgt cggcaagcga
     7861 ggagggccag ccggtacgcc tgaggaccag aagatggaga tcgtcaaagg ctggttggag
     7921 gtgcgcggcc ggatgaatca ggaagtgtat gcccatagtc gggcggtggc atcctcgacc
     7981 ctgcgacgct ggatgcggga gttgcgagag gcaggcaagt tatgagcagt tatgagcgca
     8041 ctgttatgtt agactattaa tgcttgttat ctctgctcct caatactagc gacgaagcaa
     8101 ggaggatatc atgaagactc gcttcggcgt atttgctgcg ttagcttcat tcatcctcat
     8161 cggcgtggcg gccactgctt tcgcgcataa tcaggccgac ctgaacggcc aagcattcga
     8221 ccatctgata aaccaaaacg cacgacggat ggttagagaa ggcaggcaga tcttccgctt
     8281 cgacactttt ggcgatgaag tcttctgggg cgatacgctt agattgcatg aagccattgc
     8341 gggcgaggcg cttggcggcg tgggtccggg tgtgagcccg agaacggcac tggccgtcgg
     8401 cttaaaggtc gacgtggatg cgcttcctcg agacctcgtc aggaagctga gaagtggcga
     8461 agtcgatctc gatgatccag ctacgacgct tgccttgctc aagcttaacg ctgtcgtggg
     8521 ggtgaccggc ttctttgacg aagatggaaa cctgcagtcg atgggcatcc agtgtgcttt
     8581 gtgccactcc acggtggacg actcattcgc tcctggtatt ggccgtcgcc tggacggttg
     8641 ggccaaccgc gatctcaacg tgggcgcaat cgtcgcgctt gccccaaact tgacgccctt
     8701 ggccgacctt ctgggcgtca atgaagaaac ggtgcgagcc gtgctcaata gttgggggcc
     8761 ggggaaattc gatgccgaga ttatcctgga tggtaaagct tttcagcctg acgggaggtc
     8821 ggccgctact ttgctcccgc ccgctttcgg tcttgccggt gtgaacctac acacctggac
     8881 cggatggggc tcggtcaccc actggaatgc ctttgtcgcc aatctggaga tgcatgggca
     8941 ggggacgttc ttcgatcccc ggctgaacga tccgacgcag tttccaatcg ccgcccaagc
     9001 cggtttcggg aacgtgcgga atacccccga tctgattacc tcgaagctgg ccgctcttca
     9061 cttctatcag cttgccatcc ctgcaccggc accacccgag gacagtttcg acgaagaatc
     9121 agccgcacgt ggggaggtca gcttcaacgg caaggcgaac tgtgccagtt gccacgtgcc
     9181 gcccctcttc accgagccag gctggaacat gcatacgccc gccgagatcg gtattgacga
     9241 tttccaggcc aatcgggcgc ccgatcgaca ctaccggaca tcgccgctgc agggactctg
     9301 gacacacatg aagggtggct tctaccatga cgggcgcttc gcgactctga gggacgttgt
     9361 cgatcactac aacgcctttt tcaacctggg gttgactgag gaagagatta acgatctgat
     9421 cgaatacttg aagtcgctgt aagccggcgt ttccaactgg cccgcaatcc gacgccaagc
     9481 caagcgacac tgtcctttcg gacggtgtcg ctttttgtta ggtaagaact ggcctgcctg
     9541 tgaaacagat aggcagacgt acccagtaac acttgcgtga ggaggatcag gatgtgtctc
     9601 agttgtggct gcatgatgcc tgatgatcgg cacggcgacg agcgatacat cgtgatgcag
     9661 gacatcgtcg aggcggccgc tgcgaaacga ctcgagccag gaggtgatac cacgagagga
     9721 taagacaagg cggcgcagta gtgctggcga caccaccgcg cccaacggag agccgagcca
     9781 ttacgcgaga aaggtcagcc ctgccgcatc cgtattatgc cacatctcgc acggcatgtc
     9841 agcggtgcgg cacagacata cagacgaaca aggagacaac gcaatgaaca ctatacgctt
     9901 caagattgca cacatcgcgg cggctgtttt ggtttccatc agcgcgtttg ccgccctcgc
     9961 tgccgtgaat gactacgcgt cggcagccag ctccgcagtc gttgccggcg aagtcagccg
    10021 ccatgatcat ctcgacttcg gaccgcgcga ccctctgcct ctgaaggtgc tcgccttcca
    10081 cgacgccatg cgcaagctgt gggaggacca catcacctgg acgcgaatgt tcatcgtcag
    10141 cgccctggcg gatctgcctg acgtgagcca tgctgccgag cgcctgctgc aaaaccaggc
    10201 cgacatcggc gacgccgtca agccctttta cggcgaagca gccggtgagc agttgacggc
    10261 gttgctgagg gatcacatcc tgattgccgc cgacctggtc gccgccgcca aagcgggcga
    10321 tgccgccggg gtcgaagagg cgagcgcgcg ctggtacgct aacgccgatg agatcgcagc
    10381 gttcctcaac agcgcgaacc cggacaactg gccgctcgac gagatgaagg caatgatgcg
    10441 ggagcatctc gacctcacgc ttcaagaggc ggtggcgcgc ctgaccggaa actgggcggc
    10501 agacatcgca gcttacgacg agatccacga gcagatcctg gggatggccg atatgctcag
    10561 ctcgggcatc attcgccagt ttcccaagca gttcttcaag taggtcatcg tgactgcgcg
    10621 gtgagcacgc cttcggtaag ggggtggtct cctatcgagg aggcaggaga ccaccttcta
    10681 cctcaccact ttcctcagga taggatcgcc tcaaaaatgg gtcaactgta tggatacttc
    10741 caacctgccg tggtgttcga cggcaagcat cacagcgtgg cgccagtggt tgtggatgcc
    10801 tccttgcctt ataatatcat ccacgtgctg aaccgctgcg gtgcggtcat cggattgaaa
    10861 gatcagccct gattggagga aaacatgcca gactatatgt atctcatcat cggcggcggg
    10921 atgaccgccg aggctgccgt tcgaggtatt cgcgcacttg actcgagtgg gtcgatcggc
    10981 ttgatcggcg cggagcctga accaccctac aatcgcccgc ctctgtcgaa agggctttgg
    11041 aaagggaaat ccatcgatcg catctggcgc aagacagaag gccagggagt cgagcttcac
    11101 ctggggcgga cggcgcagcg tctcgatcca agaaacaagc gcgtcaccga cgatcaggga
    11161 actctgtaca cgttcgacaa gctgctcctc gcgaccggtg gcacaccccg gcacttgccc
    11221 tttagctccg accagattat ctactatcgg accgtggagg actaccgacg cctgcgtgcg
    11281 cttgccgagc agcgacaacg atttgccgtc atcggcggtg ggttcatcgg ttcggagatt
    11341 gccgcggcgc tggcgatgaa cgggaaggat gtagtgatga tgttcccgag taagggcatc
    11401 ggtgcgcgcg tgttcccgcg ggacctgtcg ctgttcctga acgattacta ccgccaaaag
    11461 ggtgtggagg tgctggtggg agaagccgtg gtagacctgg agccacgtgc cgatcgggtg
    11521 gccttaaaga ctcgcagtgg gcgcgagatc gtagtcgatg gcgtcgtggc gggaatcggg
    11581 attcagccga atgtcgaact ggcccaggca gccggtttgg acgtggaaga cgggatcgtc
    11641 gtggacgagc tcctgcgcac gagccatccg gacgtctacg cagccggcga cgtggcattc
    11701 ttttacaacc ccatcctcgg gaagcgcctg cgcgtggaac acgagaacaa cgccaacgcg
    11761 atgggccgga tggcaggccg cgtgatggct ggcgaagtca ccccctatca tcacctccct
    11821 ttcttctact cagacttgtt cgagttaggg tacgaagccg tcggagatgt ggacgccact
    11881 ctggaggccg tcgccgactg gaaggaaccg catcacgagg gcgtcgtgta ctatctacag
    11941 gcagggcgtg tgcgcggtgt gttattgtgg aacgtccaga aacaggtgga cgcggcgcgc
    12001 cgcctggttg ccgagcctgg cccgtttcgg cctgcggatc tcaaagggcg gctgccagag
    12061 gcgcactgaa catgtcatct cgacgagaat tgtccaaggt acgaatacgc aacttgacgc
    12121 cagatagtgt ttagggtaca ctaaccctca aattcgggag ccttcgcgcg tgcggcgatg
    12181 gtgactgtca cgcgtcaggg cgaaccgggc cgggatatga aggaactggc agcgctccaa
    12241 ttggatgaga tactcgatca ggttgcgcct gccgtgcgcc gcgcgctgga ggaagacatc
    12301 ggcgacggcg acgcgacgac gctcttcacc atcccgaccg acgcgacgct cggcggccgg
    12361 tttgtcgcga aagagcctgg tgtgatcgcc gggctgtcgg tcgcgcagct tacttttagg
    12421 ctggtggacg agcgcgtgct gttcacgccg cacgtggccg acggcgattc tgtgcagcag
    12481 ggtcaggctt tcgcggtcgt cagcgggccg tgccgggcgc tgctcaccgc agagcgggtc
    12541 gccttgaact tcctacagcg gatgtcagga gtggcgacgc tcacccggcg cttcgtgcag
    12601 gccgtccagg gcacgcgggc ggtgatcctg gacacgcgca agacggcgcc cggcttgcgc
    12661 ctgctcgaca agtgggcggt gcgcatcggc ggcggccaga atcaccgttt cgggctgtac
    12721 gatatggtgc tgatcaagga taatcacatc gccgccgtgg ggagcatcag ccaagccgta
    12781 gcgcgcgtgc gcgcggccga cgatcgacgc cggccgatcg aggtggaggt taagacgctg
    12841 gccgagcttg aagaagcctt gccgctcgac gtggatcgga ttctgctcga caatatgagc
    12901 cgggacgaga tgcggcaggc tgtgcgcatc acaaacgggc gcacgcctct cgaagcgtcg
    12961 ggcaacgtga cgctagagaa cgtggcggag gtcgccgcga ccggcgtcga ttacatttcg
    13021 gccggcgcgc tcacgcactc ggtgcgagcg ctggatatca gcctgcagct cgatccgata
    13081 gtacggtaac gattgaaacg cctgctgtga attgcctgca taggccggag tgcagccctt
    13141 aggctgccta tccaggtcgg atcgcggact aaagcccgca ctccaacacg cttcagttcg
    13201 ctaactcaca gcaagcgttg aagtcgttac taccaccgga gtagaggctt tagccggaaa
    13261 ccgcctaaag gctcgactcc taggccgtaa atcaaactga aatctgctcc aagttttcgg
    13321 acgctgatta acgcggatga acgcagattg agatctaaat cagcgcaatc cgcgtttgtc
    13381 tgcgtcccta aaacgctgat ggaggaaagt atgtcagccg aagcgttata cgaacagatg
    13441 aaggcgaaac tgcacgacgt ggttccggat ttcgagctgc gccacaaggc cgtgatcgcc
    13501 gacgagatca accggctcaa agccgagaag aacgccgtca tcctggggca caattacatg
    13561 gagccggcgc tgttccactc catcccggat tacaccggcg actcgctcga gctgagccgc
    13621 atcgcggcga aaacggacaa ggacatcatc gtcttctgcg gcgtgaagtt catggccgaa
    13681 acggcgaaac tgctcaatcc cacgaagatg gtgctgctgc cttcgacgaa agccgggtgc
    13741 tcgctggcgg ccagcatcac cgccgaagac gtgcgccagc tgcgaaagcg attcccgggt
    13801 gtgccggtcg ttacctacgt caacacctac gccgacgtga aggcggagag cgacatctgc
    13861 tgcacgtcga gcaacgcgaa ggcggtcgtc gagtcgctcg gaacggatac cgtgatcttc
    13921 ctgcccgacg agtacctggc acgcaacgtc gcgagagaga cgggaaagca catcatcttc
    13981 ccgacgctgc acgccgatcc cggcgcctcg accgagctgg attaccagat gatcggctgg
    14041 cacgggcgct gcgaagtcca cgagaagttc accgtgcaag acatccgcag cgtccggcag
    14101 cagttccccg acgtgatgat cctggcccac ccggagtgca gcccggaggt ggtagctgcg
    14161 tctgattatt ccggcagcac gacggcgatg attaagcacg tccagaagac gaacgcgccg
    14221 cgctacctgc tgctgacgga atgcgcgatg ggcgacaata tcatggcggc gaacccggac
    14281 aaggaaatgc tgcggctgtg cagtgtgcgc tgcccgcaca tgaaccaaat tacgctggaa
    14341 gacacgctcg actcgctgaa gctgaaccag tatgtgatcg acgtcccgga agacgtccgt
    14401 gtgcgagccg cgcaggccgt cgagcggatg gtcgccatcg gcgcgatcgg tgggagagac
    14461 tgaccggtgg attagccaca cagtgagcga gattgtcgag actgaggtat tgatcatcgg
    14521 ctgcggtatc gcgggcggcg tcgccgcgct ccagctcgcc gacgccggca tccccgtcgc
    14581 cgtcgtcaca cgcgcgcagg agccaagcga gtcgaacacg tactacgcgc agggcgggat
    14641 catttatcgg ggcgcggacg attcgccggc gctgctggca gaggacgtgc ttcgcgccgg
    14701 cgcgggacac agcaatccga gagccgtgac gatcctggcc gaagaggggc cgcctctcgt
    14761 cagacagaca ctgatcgagc ggatcggagt gcaattcgat cgaaacgcgg gcggcgatct
    14821 atcgctggcg ctcgagggcg gccactccgt gccgcgcatc gtccacgccg ccgacgccac
    14881 cggcagggct atcgaagtcg cgctcctgga ggcgctgcgc gcgcacaaga atgtcacact
    14941 gctcaccggc cacacggcaa tcgatttgct cacgccttcc caccacgcac tgaaccggct
    15001 ggccgtgtac gagccgcagt cgtgcgtcgg cgcgtacgtc ctcgatcagg ccgccgatcg
    15061 ggtcattcgg tgcctggcgc gctacacgat cctggcgacc ggcggcctgg gacagatttt
    15121 cctctgcacg accaacccgc ccggtgcgcg cggcgacggc gtggcgatgg cctaccgcgc
    15181 cggggcgcgc gtcatcaaca tggagttcgt ccagtttcac ccgacgactt tctaccatcc
    15241 gcaagcggcg cgctttttga tctcggaagc ggttcgcggc gaaggcgccc ggctcgttca
    15301 cgcaaacggc gagccgttca tgcaaaacta cgatgcagag tggagagacc tcgcgccgcg
    15361 cgacgttgtc gcgcgcagca tctaccgcga gatgctgatg cgcgacgtgc ccaacgtgta
    15421 tctcgacctt cgctcgacca tctccgaaca gcgcatccga gaacacttcc ccaatatcta
    15481 tcaggcgtgc ctggcctacg acgtagatat cacgcgcgac ctggcacccg tcgtgcctgg
    15541 cgcacactac gcctgcggcg gcgtgtgggt cgacgaatgg ggatgcacga cgctcgatcg
    15601 attgtacgcc gtcggcgaag tggcctgcac gggcgcgcac ggcgcgaacc ggttggcgag
    15661 cacatcgctg ctcgaaggac tgctgtgggg gtatcgcacg gcgcgccaca ttctggaaca
    15721 ccgagccgag cgcgagatgc cgaacacggc cgacatcccg gcgtggcgcg acgacgggct
    15781 gaagccgccc gatccggcgc tgatccagca agacatgagc acgatcaagc agatgatgtg
    15841 gaactacgtc gggctggtgc gcacgacgcg gcgactggag cgcgcactga gcgagctgcg
    15901 ccacctggaa acggagatcg agcagtttta ccg
//
LOCUS       DBNHXB010000103        11188 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2536075, whole genome shotgun sequence.
ACCESSION   DBNHXB010000103 DBNHXB010000000
VERSION     DBNHXB010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11188)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11188)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11188
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2536075"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="AC162G_03955"
     CDS             complement(<1..360)
                     /locus_tag="AC162G_03955"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5075000.1"
                     /translation="MELNRDGRVVVEAVGLTKVYGDGRAAEVRALDNVSFRIHAAEFV
                     AVMGPSGSGKSTLLNMIGALDRPTSGQVFVNGQDIGALRDLDGFRARTVGFVFQLHNL
                     IPTLTAVENVEVPMRGQG"
     gene            complement(569..1738)
                     /locus_tag="AC162G_03960"
     CDS             complement(569..1738)
                     /locus_tag="AC162G_03960"
                     /inference="COORDINATES: protein motif:HMM:NF052765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR5075001.1"
                     /translation="MMKQASVLIVVVTIVLLAACAPAPAAEPTSANAGEVLPVVVSAS
                     GKLMPERWATLAFQAGGQVVDVSVEAGDEVKAGDVLAQLADVDARLAVAQAEAALAQA
                     EAQLAQLKAGARPEEIAAAEGAVKAADANVWAASAQLARLQSGAQAADIEAAEAALAA
                     AAMELKTAQDAYDRIKEFGGTPEEQARAALNTATHAHRAAQERVDQLKAGATKNELDV
                     ARANLAAAQAQRDAAQAQLDQIKAGATPEQIAVAEAGVKQAQVALDTAKAQLAKLKVV
                     APFGGTAGAVFVREGESIAPSQPVVTIGDLGTLRVETTDLGEVDVARVQVGQPAQVTF
                     DALPGATFSGVVTTIAPMSTPGQGGVNYTATIDLDEIEPALRWGMTAFVDIQVDR"
     gene            complement(1833..4088)
                     /locus_tag="AC162G_03965"
     CDS             complement(1833..4088)
                     /locus_tag="AC162G_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5075002.1"
                     /translation="MLKMVVKNLFRRKTRTLLTMLGIAVGVSMIVALGAMAEGIRTGY
                     LSMFSGSGSDLTLMQKGAYDITLSGVNEEVVSQIAAMPEVRDAIGMVVGNVAAADAPY
                     FFVFGYDPDGFAIERFRIVEGQPLSSSRRPASSGREIIVGEQASEALKLKVGDTLRMT
                     GGTFHVSGIYNSGSGFEDAAAILSLSDAQQLLQKHRQVGAVQVKLEDARQTERVRAQL
                     ERLYPRLAVSQSSQTADQQQMTTYIQGFAVAIALLAVIIGGIGMTNTAMMSTFERTRE
                     IGTLRALGWSRRRVLGMVFGENLALGLAGGLLGCALGAVMMALLAQSAALAFVQGQVT
                     PSLIGQGLVTALTLGAVGGLYPARWASRLLPVEALSYHGGSAKGAGARLTRLRSETLR
                     SLLRRRTRTALTVVGISIGLSAVVMLSGLADGFIVAFNQMMAGTDMDLVVRQAEATDF
                     GYSAISERDGRKIAALPGVQSVSGIVFGAITMEEMPFLLVFGYAPHEPAIEHFQIVEG
                     RSLSGSREMILGRTAADAMKVRVGDVVRMSEVGFRVVGLFETGISYEDSSSVITLRDA
                     QMLTGKPRQVSMYGVKVVDPRQAESIQAQIEASVPGVAVSPSSEFAENLPDLQATYAM
                     VWAISGLAIVVGGIGMMNTMVMSIYERTREIGALRALGWRRRRVLRMVLGESLALSLV
                     GTVAGVLLSVVFSALLSLIPMWGEWVVIVYSPGLLAQALIVAVVLGMLGGLYPSWRAS
                     NLRPAEALRYE"
     gene            complement(4269..4970)
                     /locus_tag="AC162G_03970"
     CDS             complement(4269..4970)
                     /locus_tag="AC162G_03970"
                     /inference="COORDINATES: protein motif:HMM:NF042613.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6796 family protein"
                     /protein_id="HIR5075003.1"
                     /translation="MPIDTLNAVHVTGVLAIVGAAIYAGGDVFLLAAKVNLADYPNLQ
                     PHAKLLSGAEKMVVLPWKRLAWGGLLGVFATPLVISGFWQVYQGLSPAGAGSALPPAL
                     LFACASIVGAFVHGSFMYLGEYVQALNRVSADSTAVLVGMLTRHRKIMIVTYGFLLVS
                     ITVASIWFSIVVASGRTLFPIWMAVVNPVSAFLVWFALRRILPRRISDITEGAGFNIG
                     YLIFFILTTLTLWQG"
     gene            complement(5002..6213)
                     /locus_tag="AC162G_03975"
     CDS             complement(5002..6213)
                     /locus_tag="AC162G_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR5075004.1"
                     /translation="MSENALPSTKTQSGPAGRWVWVLLPFGLLALLVVFFLATGAGIQ
                     APKGLPPVENLAVQRVTLPERGTIVVEVVNAGPDPVTVAQVLVDDAYWDFEIEPGDEI
                     PRYGTATIRLSYPWVQSEAHLIALVSSTGTLFEAEIPVAVETPLADLATVLNFALIGI
                     YVGVIPVALGLMFHPFMRGLGRRTMDAILALTIGLLVFLLIDTASEGLQVAGRVPEAF
                     NGLILFGAGAMLSYLLIQIASSRRGADRDTPAGRMNLAYLLAIGIGLHNLAEGLAIGA
                     AYAAGEAALGAFLVIGFMLHNITEGIGIAAPIVEDRPRLPSFAWLALIAGAPAILGAW
                     IGGFVYSDAAAAIFLGIGTGAIVQVIVEVGRLLARHGKREGTTFATWPNVIGFAAGLA
                     IMYATALMVTV"
     gene            complement(6210..7220)
                     /locus_tag="AC162G_03980"
     CDS             complement(6210..7220)
                     /locus_tag="AC162G_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR5075005.1"
                     /translation="MTTVSRRNILKAGTAGVLGAAGLAALATRPAQGDSPPVDVHPSH
                     REGHAGNVMVGDVDPNGVNQFDPTAMLDDWDSGMVSTLPSGQTLREYEMFASEKEIEI
                     APGVYFPAWTYNGRVPGPTIRCTEGDRIRIHFVNGTSHPHSIHFHGLHSSDMDGLDPV
                     PVGGTFTYEFDAEPFGLHQYHCHTLPLKRHIHKGLYGGFIVDPKGGRPPARELFMMMN
                     AFDTNFDGGNEVYAVNTVGFHYLRHPIQVKVGELVRIYVINIVEFDLINSFHLHANFF
                     DVYRTGTRLETSEYTDTIMLAQGERAILETRFRSPGKFMFHAHVSEFAELGWLGFFEV
                     TE"
     gene            complement(7269..8705)
                     /locus_tag="AC162G_03985"
     CDS             complement(7269..8705)
                     /locus_tag="AC162G_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HIR5075006.1"
                     /translation="MSRLDPITPNDILTTAQSLRWEIGNFHEKLMEAVYADAARIADR
                     AVTRPGGKPRFDLDRTIDRIVTSRVWGFPLMILLLTVVFWVTISGANIPSGWIADLLL
                     GTIYPLLRDGAAAIGLPWWLSGVLIDGMYLAGAWVVSVMLPPMAIFFPLFTLLEDLGY
                     LPRVAFNLDNIFKKAGAHGKQSLSMMMGFGCNAAGVIATRIIDSPRERLIAILTNNLA
                     LCNGRWPTQILIATIFIGALVPTHLAGLISALTVVGVAVLGVLLTFAVSWALSRTILR
                     GEVSTFSLELPPYRPPRILQTIYTSIIDRTLFVLWRAIIFAVPAGAVTWMSANIQIGD
                     ASIAQRLINLLNPIGMLVGLNGVILVAYIVAIPANEIVIPTVLMLTVLVTRMSGVGAG
                     AGVMFELESDADTLGLLQAGGWTLLTAVNLMLFSLVHNPCSTTLFTIYRETGSRKWTV
                     IAALLPVAIGFALTFFVAQVWRLLGGAS"
     gene            complement(8702..9538)
                     /locus_tag="AC162G_03990"
     CDS             complement(8702..9538)
                     /locus_tag="AC162G_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019854361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HIR5075007.1"
                     /translation="MTTRLPDHPTTCAGCPAHHAANLSRLGVNMENWDYVVALAGNPN
                     TGKSTVFNALTGLRQHTGNWPGKTVARAEGGFEYAGKRYKLVDLPGTYSLLATSDDEE
                     VARDFILFGQPDITLVVVDATRLERNLNLVLQVLEITDRVVVCLNLMDEARRHNLQVD
                     ERCLARDLGVPVVPTAARYREGFDLLLKTIGDVAEGRSVGRPHRIQSEPPELKHAITT
                     LVRQIESVFPGLPNARWVALRLLDGDERIGEAVRTGELGNLRPSTADAPSRFEPSLEV
                     AR"
     gene            complement(9535..10620)
                     /locus_tag="AC162G_03995"
     CDS             complement(9535..10620)
                     /locus_tag="AC162G_03995"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR5075008.1"
                     /translation="MPDPAIALLIGLLVIAAGAVIFWPNAGLVDRWQRMRQWSGRVLK
                     EDALKHIHEAEQKGQPPTLQSVAGALHVGANRTAALLVDMQEHELVTLTGGVLRLTPA
                     GRDVALHVIRAHRLWERHLAEDTSFAEADWHPLAERREHELSSDEADALFARLGHPTY
                     DPHGDPIPTANGELVPYSGRPLTALSLDETARIVHLEDEPEAVYAQLVAEGLYPGQTV
                     RLIEISPQRVRFWANGDPLRDPSAALRTGAAGAGEHVLAPIVADNISVAPIRPEADVA
                     EPAGEPLHALKPGEKAAVVRISPRCRGSERRRLMDLGLLPGTIVEAELVSPGGDPTAY
                     RVRGAVIALRKEQAQFIHITRTTGATP"
     gene            complement(10709..>11188)
                     /locus_tag="AC162G_04000"
     CDS             complement(10709..>11188)
                     /locus_tag="AC162G_04000"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR5075009.1"
                     /translation="VTLTTAGEKIALEVVRHHRLIELFLMETLGYTWDEVHQEADRLE
                     HVISEDFEERVAARLGNPQIDPHGDPIPAKDGHVTAVEQQSLIELETGQSAMVVRVSD
                     TDPEALRYAAGLGLRPQVRVTLVDAEPFGGSLRVRVENAVRSVGRDLARQVYVAVEG"
BASE COUNT         2015 a         3844 c         3448 g         1881 t
ORIGIN      
        1 gccctggcca cgcatcggca cctcgacgtt ctcgactgct gtcagcgtcg ggatcaggtt
       61 gtgcaattgg aacacgaagc ccaccgtgcg cgcgcggaat ccatccaggt cgcgcagcgc
      121 gccgatgtct tgcccgttga cgaagacctg cccagacgtc ggcctgtcca gcgcgccgat
      181 catgttcagc agggtcgact tgccggaacc gctcggcccc atcacggcga cgaactcggc
      241 ggcatgaatg cgaaagctca cattgtccaa ggcccgcacc tccgccgccc ggccatcgcc
      301 gtagaccttc gtcaatccca ctgcctcgac gaccactcgc ccgtcacgat tcagttccat
      361 tcttgactcc gcagaatagc cacggagaca ctgagtcaca gagagcgcgc tgaatgcacc
      421 taaaggtaac tactcagccc ttcgtgctgg aatcgaggct ttagccggtt tcatgtccgg
      481 caagagcttc cggctaaagc ctcgactccg gatcgctggc aggctagttg ttacgaatct
      541 ccgtgtcctc tgtgtctctg tggcaagact atcgatccac ctggatgtcg acgaacgccg
      601 tcatgcccca gcgcagtgca ggttcgattt catccagatc gatggtggcc gtgtaattga
      661 cgccaccctg ccccggcgtc gacatcggcg cgatggtcgt cacgacgccg gaaaacgtcg
      721 cacccggcag cgcatcgaac gtcacctgcg ccggctggcc gacctgtacg cgcgccacgt
      781 cgacctcgcc gagatcggtc gtctcgacgc gcagcgtgcc cagatcgccg atggtcacga
      841 ccggttggct tggcgcgatc gactcgccct cgcgcacgaa caccgcgccg gcggttccgc
      901 caaacggcgc gacgaccttc agcttggcga gctgggcctt cgccgtgtcc agcgccacct
      961 gcgcctgctt cacacccgct tcggcgacgg cgatttgctc cggcgtcgcc ccggctttga
     1021 tctgatcgag ctgcgcctgc gccgcgtccc gctgcgcctg tgctgccgcc aggttggcac
     1081 gcgccacatc cagctcgttc ttcgtcgccc cggccttcaa ttgatcgaca cgctcctgcg
     1141 cggcgcgatg cgcatgcgtc gccgtgttca gcgccgcgcg agcctgctcc tccggcgtgc
     1201 cgccgaactc tttgatgcga tcgtaggcat cctgcgctgt cttcagttcc atcgcggcgg
     1261 cggcgagcgc cgcttcggca gcctcgatgt cggcggcctg cgcacccgat tgcagtctcg
     1321 cgagctgcgc cgaagccgcc cagacattcg cgtcggcagc cttgacggct ccctcggccg
     1381 ccgcgatctc ttcgggtcgc gcgccggctt tcaactgggc gagctgcgcc tcggcctgcg
     1441 cgagcgccgc ctcagcctgc gccacggcca gccgggcatc cacatcggcg agctgcgcca
     1501 gcacatcgcc ggctttgacc tcgtcgcccg cttcgacgct cacgtcgacc acctgtccgc
     1561 ccgcctgaaa cgcaagtgta gcccaacgct cgggcatcag ctttcccgaa gcggatacga
     1621 cgacgggtag cacttcccct gcattggccg acgtcggctc ggcggccggc gccggtgcgc
     1681 acgcagccaa cagcacgatt gtcacgacga cgatcaaaac gctcgcttgc ttcatcatca
     1741 aactcctcat cagagccgaa tacctgtgca tgcttcgcgc ggtcacactg ccagccgctg
     1801 tctgccatct gctatctgcc atctgctatc cgctactcat agcgcagcgc ctccgccggt
     1861 cgcaggttcg acgcgcgcca cgacgggtac agcccgccga gcatcccgag cactacggcg
     1921 acgatcagcg cctgcgccag aagaccgggc gagtacacga tcaccaccca ctcgccccac
     1981 atcggaatga gagagagcag ggcgctgaac acgacagaca gcaggacgcc ggcgaccgtg
     2041 ccgaccaggc tgagcgccag cgactctccg agcaccatgc gcagcacgcg ccgccgccgc
     2101 cagccgagcg cgcgcagcgc gccgatctcg cgcgtccgct catagatgct catcaccatc
     2161 gtattcatca tgccgatacc acctacgacg attgccagcc cgctgatcgc ccacaccatc
     2221 gcgtatgtcg cctgcaagtc tggcaggttt tccgcgaatt cactcgacgg ggacacggcc
     2281 acgccgggga cggacgcctc gatctgcgcc tggatcgatt cggcctgccg gggatcgacg
     2341 actttgacgc cgtacatcga cacctggcgt ggcttgccgg tcagcatctg cgcgtcgcgc
     2401 agcgtgatca cgctgctgct atcctcgtag gagatgcccg tctcgaagag accgacgacg
     2461 cggaagccga cctcgctcat ccggaccacg tcgcctacgc gaaccttcat agcatccgcc
     2521 gccgtacggc ccaagatcat ttcacgactg ccggataagc tccgcccctc gacgatctgg
     2581 aaatgctcga tggccggctc gtgaggcgca tagccaaaca cgagaagaaa cggcatctct
     2641 tccatcgtga tggcgccgaa gacaatgccc gatacgcttt gcacgcccgg cagcgccgcg
     2701 atcttgcggc cgtcccgctc gctgatcgcg ctgtaaccga agtcggtcgc ctccgcctgg
     2761 cgcaccacca ggtccatgtc ggttccggcc atcatctgat tgaaggcgac gatgaatcca
     2821 tctgccaggc cgctcaacat gacgactgcg gacagcccga tgctgatccc gacgacggtc
     2881 agcgccgtgc gcgtgcggcg ccgcagtaga gaacgcagcg tctcggagcg cagcctggtc
     2941 aggcgcgcgc cggcaccttt cgcgctcccg ccgtgatagc tcagcgcctc gacaggcagc
     3001 agccttgacg cccagcgagc cggatagaga ccgccgaccg cgcccagcgt cagcgccgtc
     3061 actagcccct gtccgatgag cgatggcgtg acttggccct gcacaaaagc cagcgccgcg
     3121 ctctgggcca gcaatgccat catcaccgcg cccagcgcgc atccgagcaa cccgcccgcc
     3181 agtcctagcg cgaggttctc accgaacacc atgcccagca cgcggcggcg gctccagccg
     3241 agcgcgcgca gcgtgccgat ctcccgtgtg cgctcgaacg tgctcatcat cgccgtgttc
     3301 gtcatgccta taccgccgat gatcaccgcc agcagcgcga tcgcaactgc gaaaccttga
     3361 atgtaggtgg tcatttgctg ctgatcggcg gtctggctcg actgcgacac ggccaggcgc
     3421 ggatacagcc gctcgagctg cgcgcggact cgttcggtct gccgcgcatc ctcgagcttg
     3481 acctgcacgg cgcccacctg gcggtgtttc tgcagcaact gttgcgcatc gctcagcgac
     3541 aaaatcgccg ctgcatcctc gaagccgctg ccgctgttgt agataccgct gacgtgaaat
     3601 gtgccgccgg tcatgcgcag cgtgtcgccg accttcagct tcagcgcctc ggaagcctgc
     3661 tcgccgacga tgatctcgcg gccgctagaa gcgggccgtc gcgacgagga aagcggctgc
     3721 ccttcgacga tgcggaaccg ctcgatggcg aacccatctg gatcgtagcc gaacacgaag
     3781 aaatacggcg cgtcggccgc ggcgacgttg ccgacgacca tgccgatggc gtcgcgcact
     3841 tccggcatgg ccgcaatctg gcttacgact tcctcgttca ccccgcttag tgtgatgtcg
     3901 tacgcgcctt tctgcatgag tgtcaggtcg gacccactgc cggagaacat cgacaggtaa
     3961 ccggtgcgga tgccctcagc catcgcgccg agcgcgacga tcatcgacac gcccacggcg
     4021 atgccgagca tcgtcaggag cgtgcgcgtc ttgcgtctga acaggttctt gacaaccatc
     4081 ttcagcatag gccgctcatc ccagtacact cacatataca ggccgcatgt cgaatctccg
     4141 agcaccgtag tgccgaaggt cgcccaagag tatacgccca ctgcacgaca aaggtcatcc
     4201 ccgcgatctg gcagatacct gacggcaacc ggccaacaaa aacccaggcc cggcatggtg
     4261 gatgcccatc acccctgcca caaagtcagc gtcgtcagaa tgaagaatat caggtagccg
     4321 atgttgaacc cggccccttc cgtaatgtcg cttatccgcc tgggcaaaat ccggcgtagg
     4381 gcgaaccaga ccagaaacgc cgacaccggg ttcacgacgg ccatccaaat cggaaagagc
     4441 gtgcggcctg acgcgacgac gatcgagaac cagatggagg cgacggtgat agacacgagc
     4501 aggaagccgt acgtcacaat catgattttc cggtggcggg tcaacatgcc gaccaagacg
     4561 gctgttgaat cggcgctcac gcggttcaac gcctgcacgt attcacccag atacatgaac
     4621 gagccatgca cgaacgcccc gacgatcgaa gcgcaggcga agagcagggc cggcggcagc
     4681 gcgctgcctg cgccggcagg cgacaggccc tggtatacct gccagaagcc ggaaatgacc
     4741 aggggcgtgg cgaatacgcc cagcagccca ccccacgcca gtcgcttcca cggcaagacg
     4801 accatctttt ctgcaccgga cagcaacttg gcgtgaggct gcaagttcgg gtaatcggcg
     4861 aggttgacct tagctgcgag caagaagaca tcgcccccgg catagatcgc tgcgccgacg
     4921 atggcgagca ccccggtcac atgtacggca ttgagcgtat cgatgggcat cggtatagtc
     4981 cgtatgacgt gcgtatcgcc actacacagt gaccatcagc gccgtcgcgt acatgatcgc
     5041 cagcccggcg gcgaacccga tcacgttcgg ccacgtggcg aaggtcgttc cctcgcgctt
     5101 gccgtggcgc gccagcagcc tgcccacttc gacgatgacc tgcacgatcg cgcctgtgcc
     5161 gatgcccagg aagatcgccg ccgccgcgtc gctgtagacg aagccgccga tccacgcgcc
     5221 gaggatggcg ggcgcgccgg cgatcagcgc cagccaggcg aagctgggca gcctgggccg
     5281 gtcctcgacg atcggcgcgg cgatgccgat gccctcggtg atgttgtgca gcatgaagcc
     5341 gatcacgagg aacgcgccga gcgccgcttc gccggcggcg tacgccgcgc cgatggccag
     5401 cccctcggcc aggttgtgca ggccgatgcc gatcgccagc agatatgcga gattcatccg
     5461 cccggccggc gtatcgcgat ccgcgccgcg ccgcgagctg gcgatctgga tcagcaggta
     5521 cgacagcatc gcgcccgcgc cgaacaggat caatccgttg aacgcctcgg gcacgcgccc
     5581 cgcgacctgc aatccttcgc tcgccgtgtc gatcagcaga aacaccaaaa gcccgatcgt
     5641 cagcgccagg atcgcatcca tcgtacggcg gcccagcccc cgcatgaagg ggtggaacat
     5701 cagacccagt gcgaccggga tcacgccgac gtagatgccg atcagcgcga agttgaggac
     5761 agtcgccaga tcggccaacg gcgtctcgac ggcaaccgga atctcggcct cgaacaatgt
     5821 gccggtcgaa gagacgagcg cgatcaggtg cgcctcgctc tgcacccacg ggtacgacag
     5881 gcgaatcgtc gccgtgccgt aacgcgggat ctcatccccc ggctcgatct cgaagtccca
     5941 gtacgcgtcg tcgacgagca cctgcgcgac cgtcaccggg tcgggcccgg cgttgacgac
     6001 ctcgacgacg atcgtccctc gctcgggcag cgtcacccgc tgcaccgcga ggttttcgac
     6061 cggcggcaag cccttcggcg cttgaatgcc cgcgccggtc gccaggaaga acacgacgag
     6121 gagcgcgagc agcccgaacg ggagcagcac ccacacccag cgccctgccg gcccggattg
     6181 cgtctttgtc gaaggcaaag cgttctcgct cattccgtca cctcgaagaa gcctagccag
     6241 cccagctcgg cgaattcgct cacgtgcgcg tgaaacatga acttgccggg cgatctgaag
     6301 cgcgtctcca ggatcgcgcg ctcgccctgc gccagcatga tcgtgtcggt gtactcgctc
     6361 gtctccagcc gcgtgccggt acggtacacg tcgaagaagt tagcgtgcag gtggaacgag
     6421 ttgatcaggt cgaactcgac gatgttgatc acgtagatgc gcaccagctc gccgaccttg
     6481 acctggatcg gatgccgcag gtagtgaaag ccgacggtgt tgaccgcgta cacttcgttg
     6541 ccgccgtcga agttcgtgtc gaacgcgttc atcatcatga acagctcgcg cgccggcggg
     6601 cggcctccct tcggatcgac gatgaagccg ccgtacaggc ccttgtggat gtggcgcttg
     6661 agcggcagcg tgtggcagtg atactgatgc agcccgaacg gctcggcgtc gaactcgtac
     6721 gtgaacgtgc cgccgaccgg caccggatcc agaccgtcca tgtccgacga gtggaggccg
     6781 tgaaagtgga tggagtgcgg gtgcgacgtg ccgttgacga agtggatgcg gatgcgatcg
     6841 ccctccgtgc agcggatcgt cgggccgggc acgcgcccgt tgtacgtcca ggccgggaag
     6901 tacacgcccg gcgcgatctc gatctccttc tcgctggcga acatctcgta ctcgcgcagc
     6961 gtctggccgc tgggcagtgt gctgaccatg ccagagtccc aatcgtcgag catcgccgtc
     7021 gggtcgaact ggttgacgcc gttcgggtcg acgtcgccga ccatcacgtt gccggcatgc
     7081 ccttcacgat ggctcggatg cacgtcgacc ggcggtgagt cgccttgcgc cggccgcgtc
     7141 gccagcgcgg caagcccggc cgcgccgagc acgccggccg tgcctgcctt cagaatattc
     7201 cttctcgata cagttgtcat cgtgtttcac tccttccttg gctatgatta cgttttacgt
     7261 ttcacgtttc agcttgcccc acccagcagc cgccacacct gcgccacgaa aaacgtcagc
     7321 gcaaacccta tcgccaccgg cagcagcgca gcgatcaccg tccacttgcg gctgcccgtc
     7381 tccctgtaaa tcgtgaacaa ggtcgtgctg cacggattgt gcaccaggct aaatagcatc
     7441 aggttgaccg ccgtgagcag ggtccagccg ccggcttgga ggagacccag cgtgtcggcg
     7501 tcggactcca gctcgaacat cacgcctgcg cccgcgccca cgccgctcat gcgcgtgacc
     7561 agtacggtca gcatcagcac cgtggggatc acgatctcgt tggccggaat ggccacgatg
     7621 tacgcgacga gaatcactcc gttcaggccg accagcatgc cgatcggatt gagtagattg
     7681 atcaggcgct gagcgatgct cgcgtcaccg atctggatat tcgcgctcat ccaggtcacg
     7741 gctccggcgg ggacggcgaa gatgatcgcc cgccacagca cgaacagcgt gcgatcgatg
     7801 atggaggtgt agatcgtctg gagaatgcgc ggcggccggt acggcggcag ctccaggctg
     7861 aaggtcgaga cctccccgcg caggatggtc cgcgacagcg cccacgacac ggcgaaggtg
     7921 agcagtacgc ccagcactgc cacgcccacc acggtcagcg ccgagatcag cccggcgagg
     7981 tgagtgggaa ccagcgcccc gatgaagatg gtggcgatga gaatctgcgt cggccagcgg
     8041 ccgttgcaca gcgctaagtt gttggtgagg atggcgatca atcgctcgcg cgggctgtcg
     8101 atgatgcgcg tggcgatgac gcccgccgcg ttgcagccga agcccatcat catgctcagc
     8161 gactgcttgc cgtgcgcccc ggccttcttg aagatgttgt ccagattgaa ggcgacgcgc
     8221 ggcaggtaac ccaggtcttc cagcagcgtg aacagcggga agaaaatcgc catgggcggc
     8281 agcatcacgc tgaccaccca cgccccggcc agatacatcc cgtcgatcag cacgccgctc
     8341 agccaccacg gcaggccgat agccgccgcg ccgtcgcgca gcagggggta gatcgtgcca
     8401 aggagcagat cggcgatcca gccggagggg atattcgctc cgctgatcgt gacccagaac
     8461 acgaccgtga gcagcaggat catcagtggg aagccccata cccggctggt cacgatacgg
     8521 tcgatcgtgc gatcgaggtc gaagcgaggc ttaccgccgg ggcgggtcac ggcgcggtcg
     8581 gcgatgcggg cggcgtcggc gtacaccgct tccatcagct tctcgtgaaa gttgccgatc
     8641 tcccagcgta gcgattgagc agtggtgaga atgtcgttcg gtgtgatcgg gtcaaggcgg
     8701 ctcatcttgc cacctccaga ctcggttcga accgagaggg tgcatcggcc gtcgatggcc
     8761 gcaggttgcc gagttcgccg gtgcgcacgg cttcgccgat gcgctcatcg ccgtcaagca
     8821 gtcgcagcgc cacccagcgc gcgttgggta gccctggaaa aaccgattcg atttgcctca
     8881 cgagcgtggt gatcgcgtgt ttaagctcgg gcggctcgct ttgaatacga tgcgggcggc
     8941 cgacgctgcg gccctcggcc acgtcgccga tcgtcttcaa tagcaggtcg aacccttccc
     9001 ggtagcgggc ggccgtcggc acgaccggaa cacccaggtc gcgcgccagg cagcgttcgt
     9061 cgacctgcag gttgtggcgt cgtgcctcgt ccatcaggtt cagacacact acgacgcgat
     9121 cggtgatctc cagcacctgt agcaccaggt tcaagttgcg ttccagccgc gtggcatcga
     9181 cgacgaccag cgtgatgtcg ggctggccga aaagaataaa atcgcgtgcc acctcttcgt
     9241 cgtcgctggt cgccaacagc gaatacgtgc cgggcaggtc caccagcttg tatcgcttgc
     9301 cggcgtattc gaacccgccc tcggcgcgcg cgacggtctt gcccggccag ttgcccgtgt
     9361 gttggcgcag acccgtgagc gcattgaaca cggtgctctt cccggtgttg gggttacctg
     9421 ccagcgccac cacgtagtcc cagttctcca tgttgacgcc caaccgcgac aagttggcgg
     9481 catggtgcgc cggacagcca gcacaggtag tcgggtggtc gggtagtcga gtggtcatgg
     9541 tgtggccccg gtcgtgcgtg tgatgtggat gaactgcgcc tgctctttac ggagagcgat
     9601 gaccgcgcca cgcacacgat acgccgtcgg atcgccgccc ggactcacta gctcggcctc
     9661 gacgatcgtg cccggcagca ggccaaggtc catcagccgc cggcgctccg atccacggca
     9721 gcgcggcgag atgcgcacga cggcagcctt ctcgccgggc ttcagcgcat gaagcggctc
     9781 gcccgccggc tctgcgacgt cagcctccgg tcgtatcggc gcaaccgaaa tgttgtcggc
     9841 cacgatgggc gccaggacgt gctcgcctgc ccccgcagcg cctgtcctga gcgcagccga
     9901 aggatcgcgg agggggtcgc cgttggccca gaagcggacc cgctgcggtg agatctcgat
     9961 cagccgaact gtctggccgg gatacagccc ttccgccacg agctgggcgt acacggcctc
    10021 cggctcgtct tcgagatgga cgatgcgcgc cgtctcgtcg agcgacagtg ccgtcagcgg
    10081 acgaccgctg tacggaacca actcgccgtt ggcggtcggg atgggatcgc cgtgcgggtc
    10141 gtacgtgggg tgtcccagcc gcgcaaagag cgcgtccgct tcgtcggacg atagctcgtg
    10201 ctcgcggcgc tcggccagcg ggtgccagtc ggcttcggca aagctggtgt cctccgccag
    10261 gtggcgctcc cacagccggt gcgcacggat gacgtgcaat gctacgtccc gcccggccgg
    10321 cgtcaggcga agaacgccgc ctgtgagtgt caccaactcg tgttcttgca tatcgaccag
    10381 caaagcagcc gtccgattgg cgcccacgtg cagcgcgccg gccacgcttt gcagggtggg
    10441 gggctggcct ttctgttcgg cttcgtgaat gtgtttaagc gcgtcttctt tgagaactcg
    10501 gcccgaccac tggcgcatgc gctgccagcg gtcgacaagc ccggcattcg gccagaagat
    10561 caccgcgcca gcggctatga ccagcaaacc gatcaacaac gcgatcgccg gatcaggcat
    10621 ctcatactcc aaaccctagc ggaggcggct cccatgccgc cggatggctg ggcgtgggag
    10681 cccagcccgc tggttttcgg ataggcactt aaccctccac cgcgacgtaa acttgccgcg
    10741 ccaggtcgcg gccgaccgag cgcacggcat tctcgacacg cacgcgcagc gatccgccga
    10801 acggctcggc atcgactagc gtaacacgca cctgcggacg caggccgagg ccggccgcgt
    10861 aacgcagcgc ttccgggtcg gtgtcgctca cgcgcacgac catggccgac tggcccgtct
    10921 ccaattcgat taagctctgc tgttcgacgg cggtcacgtg gccgtctttc gcgggaatcg
    10981 ggtcgccgtg tggatcgatc tgcgggttgc ccagccgggc cgccacgcgc tcctcgaagt
    11041 cctcgctgat cacatgctcc aaccgatcgg cctcctggtg aacttcatcc caggtgtagc
    11101 ccagcgtctc catcaagaac aattcgatga gccggtgatg gcggacgacc tccagcgcga
    11161 tcttctcgcc ggcggtagtc agcgtcac
//
LOCUS       DBNHXB010000104        11963 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4381805, whole genome shotgun sequence.
ACCESSION   DBNHXB010000104 DBNHXB010000000
VERSION     DBNHXB010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11963)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11963)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11963
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4381805"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..768
                     /locus_tag="AC162G_04005"
     CDS             157..768
                     /locus_tag="AC162G_04005"
                     /inference="COORDINATES: protein motif:HMM:NF016071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR5075010.1"
                     /translation="MNTSTRINRHLLRLGATLLLAAGLSSAVLAVGAKPLPPDDGSNG
                     ILGVVDHVCPDAYDDEVPVGEPTDDRVENASTLVAGAPQSHNFDGNTLLGVADKDWAR
                     FEVVRTGVYTLTTSNLSAQTDTAIELYDAVGSLAAANDNAAGPASQIVWTAPETASGW
                     YYLSVFPTGTTPYANCAGMVVSYTLSLQSKEPVFLLLPLVMRN"
     gene            complement(863..2344)
                     /gene="der"
                     /locus_tag="AC162G_04010"
     CDS             complement(863..2344)
                     /gene="der"
                     /locus_tag="AC162G_04010"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR5075011.1"
                     /translation="MNKPLVAIVGRPNVGKSTLFNRLIGERLAVVDDTPGTTRDRLQA
                     DAEWNGRVFTVVDTGGIEVLPRRQPPPLRSGGLREPSPPAPLPSKTTGEGSAHTPLAV
                     ASAQYIPQIRRQAEIAVAEADAILFLVDVEAGITSADQEVADTLRARRRQRPEGEVPI
                     FVVANKVDNVAREQAALEFYALGLGEVYPVSAIHGIGTGDLLDALVETFPPQEAEEEE
                     SVKIAIVGRPNVGKSSLLNALLGEERAIVSPVPGTTRDAIDTRLEIGELAVTLIDTAG
                     IRRRGHIEPGVEQFSVLRALRAIKRCDVALLLIDAVEGVTAQDAHIAGFILEEAKSVV
                     LIVNKWDLYESTSQRVGESARRATQKEFEQALRAALNFLDYVPALFISAKTGLRVDQV
                     LPLALHVQEERLARIPTAELNRIVREAVDRQAPPTKAGKRLKIYYTSQVRADPPTFLF
                     HVNDTTLVHFSYERYLENRIREAYPFTGTPLRLSFRLRSQSRE"
     gene            complement(2341..3597)
                     /locus_tag="AC162G_04015"
     CDS             complement(2341..3597)
                     /locus_tag="AC162G_04015"
                     /inference="COORDINATES: protein motif:HMM:NF019561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR5075012.1"
                     /translation="MLRWLARNLWNILLSVILAVIVWVVAVNEANPSSERTFELIPIA
                     FVNQPPDTVVYDVSASTVDVTVSAPRETWADLNDKVITATVDLSQPRESYPVQASVLD
                     SNVRPVGIVHVEPPSVSAKVEPRDSAQFSVEVNLVGEPARTYKIDSVATQPPTATVTG
                     PASSVSQVAKVASEFSVQGLSQPVSQTIALKVLGADGQVVPNVQVIPDRVRLSVDFEQ
                     QAGFRNLSVKVEMTGTQASGYRLAGVDVIPPTVTLIGSSNALESLPAFVATELIDISG
                     AQTDIEKDVPLVLPPDVALFDRQSVRVVVKIEPIVGSLTIPSQPIAIGLQPGLAVRVS
                     PETVGVILEGALPVLETIDLGQDVRVILDLSDLGIGTHQIEPRVETPEGVVARSVLPA
                     TVQVIIERAPRGTPTPTVTPLPTRRP"
     gene            complement(3638..4513)
                     /gene="cdaA"
                     /locus_tag="AC162G_04020"
     CDS             complement(3638..4513)
                     /gene="cdaA"
                     /locus_tag="AC162G_04020"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR5075013.1"
                     /translation="MADLLAVLDRLEWGSLLDIALVALVFFGALTLVRGTQADTLLRG
                     VLTLVVIGALLGSAFSFPALGWLLSRAGFALLVAIPVIFQPELRRALERLGRAGPRFG
                     QTARDVASIESVISAVAQTATRLADRRQGALIVLERETGLQSYADTGVRLDAEVTPEL
                     LQTVFYTGTMLHDGAAIIRQDRILAAACILPLTSAFLSDRRMGLRHRAAIGITESTDA
                     VAVVVSEEHGHVSIAHNGRLIRRIEPRRLEDVLRTFYQSELKETSPRWLSRLRLPRLT
                     SGRRQSPTRPSNPDR"
     gene            complement(4515..6653)
                     /locus_tag="AC162G_04025"
     CDS             complement(4515..6653)
                     /locus_tag="AC162G_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5075014.1"
                     /translation="MPGDRQAYQQALQRAAGHVAAKAWARAIAEYQKALTQFPDDEET
                     WSKVADALERIGRLDGAARAYAAIADLRLRRNDVDAAVDIWVRAARLDPNNTDIQRRL
                     ARVYRHQGKTKLAIRAHLALARIHNKRGQFQEAVEQVQAAATLDPDDPDILTAMELLR
                     GGQPAPPAPPPARKVDTSIFDLPIEEEAEELRGSPVEIAREKALSELAGSIFEEVPPT
                     QQLSKAEIDALVGQAIDYQTSGEVQAAIDAYQRIIKAGAQMPAAHFNLGLLLQETMHF
                     DEAIQEFQRTVDAPEYRLGSLFALGECYRAKGHVDEALSYFLEVLKIIDLGTVRREQA
                     DDLIQVYQSLAESYAAKGDSEQATQFADALVEFLKSKGWEDKVGVARKRLDQLIIEGG
                     PAITLAELLTVPNPTEMLEAMALIGEYAKREKLYSALEIAETAILGAPYYLPLHLRLG
                     DILWSCGRAEAALAKYLAVADTYSARGEARLAIGTYQRILKMAPMDVNVRSKLIELLV
                     SYGQIDRALEHYLQLADAYYQLADYNKARDVYQDALKLAPRGSAQRNWTVQVLHKLGD
                     IHVQRIDWRHALAIYEQIKSIAPGDERAHVALIDLYFKTNQPLRAIKEIDELVAAYKS
                     SGKTDKVIPILDDQVRAHPTDMALRARLAQACIENQHIPEGIQQLDALGDLQINAGMK
                     REAMATIRAIVALNPPNAAEYRQLLAQLGG"
     gene            complement(6903..7805)
                     /gene="argF"
                     /locus_tag="AC162G_04030"
                     /pseudo
     CDS             complement(6903..7805)
                     /gene="argF"
                     /locus_tag="AC162G_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011713.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
     gene            8139..9089
                     /locus_tag="AC162G_04035"
     CDS             8139..9089
                     /locus_tag="AC162G_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5075015.1"
                     /translation="MIDVVDLTKSYGETRALRGVSFHVAEGEIVGLLGPNGAGKTTLM
                     KILTGYLHPDEGEASVDGLDVLTHTLEVQARIGYLPENAPLYPELSIQSYLRMMADLR
                     RIPQADQLDRISEAVHATSLADHLTRPIGELSKGYRQRVGLAQALLHKPRLLILDEPT
                     IGLDPTQIIEIRSLIRRLARHSTILFSTHILPEVEALCDRAIIIMNGRIRADARLSEL
                     ASTSDAILVLQDDVKGVESALREMSGVRGVEPMKVLDGYPAFRVLGAERVDLCPSVYD
                     VARQNAWPLRELRRDVRTLETVFNQLATTAFEDEDEPEGL"
     gene            9093..>11963
                     /locus_tag="AC162G_04040"
     CDS             9093..>11963
                     /locus_tag="AC162G_04040"
                     /inference="COORDINATES: protein motif:HMM:NF021351.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HIR5075016.1"
                     /translation="MKQISSITRKELDSYFSSPMALIFVGVFLAFTLGVFFWGPEAFF
                     ARGIADVRPLFRWMPVLLIFLVAALTMRQWSEEERSGTLEMLLTLPVRPAQLVLGKFL
                     AVMALIAVALALTLPLPLSISLLGNLDWGPVIGGYLAALLMAGAYAAIGLFVSSRTDN
                     QIVALIATVLLGGLFYVAGTGFMTDFAGGALGDALRALGTGSRFESIERGVVDARDLL
                     YYLSLTVFFIVLNVVSLDSKRWGAGERGRVHRRDIGLTAVLVGLNLVLLNTWSYTLQG
                     LRVDLTQQQEFSLSQTTRDLLASLQEPLVMRAYISDKTHPLLEPLKPRIADMLREYAV
                     ASAGKITADVVDPASDPAIEAEANQTYGIEPTPLQVSGRYEASLINSYFDVLVRYGDQ
                     NTVLNFRDLIEVQSNRDGTLDVRLRNLEYDLTRSIKKVLYGFQSVDAVLAAMEEPVQL
                     TLVVTPDTLPEALQSAPETIRKVAEDIAAQSNGKFTFEEVNPDEAGSSLTRQALVDTY
                     KLQPIPVSLFSEDSYYLDMLLHIGDQTRILSPGSDVSDAGVRSAIESALKRSSTGFLK
                     VVGLWTPPATPTTNVLGQPQQPISDWQLIRQQLGQDYTVRSVDLSSGQAPPDVDTLVV
                     IAPQNLTDRERFAIDQYLMRGGSVIVAASNYAITPDPFGGGLALQPLENGLREMLQHY
                     GVDVGQSLVLDPQNEPFPTYVARNVGGLVVQELQAINYPFFPDVRADGMDPESPIVAR
                     LPAVTMNWASPVELDAEKNAKRQRSVLLRSSPLAWLTTNTNIQPDFQSYPELGFPVEG
                     EPKSYPLAVSVQGVFQSYFKGKPSPFQPEAGSPLDTSSQPSLEPTPTPAPAQPVSVID
                     ESPDTARLVVIGSAEFVDDVVLQISSNLTRDRYLNNLQFLQNAVDWSVEDIDLLGIRA
                     RGTLTRVLKPMEQREELTWELLNYGIALLALIGIWMIWRARRR"
BASE COUNT         2071 a         3895 c         3809 g         2188 t
ORIGIN      
        1 gattacagtg tctcgatgac ggtcgagccg agatactcgc tcctgttgcc gctcatcatg
       61 aggagctacc cgtagcccag tgatcagtaa tcagtgatca gtaatcagta acatgtcctt
      121 tgacggatcg gcccggaaat gcaaggagtg cacctcatga acacgagtac tcgaatcaac
      181 cggcatctcc tgcggctcgg cgcaacgctc ttgctggcag ccggcctgtc aagtgcagtg
      241 ttggcagttg gggccaagcc cctgccgcct gacgacggca gcaacggcat cctcggcgtc
      301 gtcgaccacg tctgtccgga cgcgtacgac gacgaggtac ccgtcggcga gcctaccgac
      361 gatcgagtcg agaacgcgag cacgctcgtc gccggcgcgc cgcaatcgca caacttcgac
      421 ggcaatacgc tcctcggcgt ggccgacaag gactgggcgc gtttcgaggt ggtgcgcaca
      481 ggggtgtata cgcttaccac gtccaatttg agcgcgcaga ctgacacggc gatcgagttg
      541 tacgatgccg ttggcagcct ggcggctgcg aacgacaacg cggcagggcc tgcctcgcag
      601 atcgtgtgga cggcgccgga gacggcttcc ggctggtatt acctgtcggt cttccccacg
      661 ggcaccacgc cttacgcgaa ttgcgccggc atggtggtga gctacacgct gtcgctgcag
      721 agcaaggaac cggtgttcct cctccttccg ctggtcatgc gcaactagcc ttgatcgtct
      781 gatgtcctga cgaaagcctc ccgcaggcgc ttggcctgcg ggaggctgtt tttcgctgag
      841 aagttgtctc ttcagtcgct tgtcactctc gactttggct gcgcagcctg aagctcaacc
      901 tcagcggcgt gcccgtgaac ggataagcct cgcgaatccg gttttccagg tagcgctcgt
      961 acgagaagtg caccagggtc gtatcgttga cgtgaaacag gaacgtcggc ggatcggcgc
     1021 gcacctgcga ggtatagtaa atcttcagtc gcttgccggc cttcgtcggc ggagcctgcc
     1081 tgtcgaccgc ctcgcgcacg atgcgattca gctcggcggt cgggatgcgg gcaaggcgct
     1141 cctcctgcac gtgcagcgcc agcggcagca cctgatcgac gcgcagaccg gtcttggccg
     1201 agatgaacag cgcgggaaca tagtcgagaa agttgagcgc ggcccgtaac gcctgctcga
     1261 actctttctg agtcgcgcgc ctcgctgact cgccgactcg ttgactcgtc gactcgtaca
     1321 aatcccactt attgacgatc aacaccacgc tcttcgcctc ttcgagaata aacccggcga
     1381 tgtgcgcatc ctgagccgtg acaccctcga ccgcatcgat cagcagcagc gccacgtcgc
     1441 agcgcttgat cgcccgtagc gcgcgcagca cgctgaactg ctcgacgccc ggctcgatgt
     1501 gtccccttcg acgaatgccc gccgtgtcga tgagcgtgac ggccaattct ccaatctcta
     1561 atctcgtatc aattgcatct cgcgtcgttc caggcaccgg gctgacgatc gctcgctcct
     1621 cgccgagcag cgcattgagc aggctgctct tgcccacgtt gggccgcccg acgatggcga
     1681 tcttgaccga ttcttcctcc tcggcctcct gcggcggaaa ggtctcgacg agcgcgtcga
     1741 gcaggtcgcc cgtgccgatg ccgtggatcg ccgagacggg atacacctcg cccaacccca
     1801 gtgcgtagaa ctccagcgcg gcctgttccc gcgccacgtt gtccaccttg ttggcgacga
     1861 cgaagatggg cacctcccct tccggtcgct ggcggcggcg cgcgcgaagc gtgtcggcca
     1921 cttcctgatc cgccgaggtg atccccgctt cgacgtcgac caggaacagg atcgcatccg
     1981 cctcggcgac ggcgatctcc gcctggcggc gaatctgtgg gatgtactgg gcggaggcga
     2041 cggccagtgg cgtgtgcgca ctcccctctc ctgtcgtctt cgacgggaga ggggccgggg
     2101 gtgagggctc tcggagacct ccggagcgga gcggaggggg ctgtcggcga ggcagcactt
     2161 cgatgccgcc ggtatcgacg acggtgaaga cgcggccgtt ccactccgcg tcggcctgca
     2221 ggcggtcgcg ggtcgtgccc ggcgtgtcgt cgacgaccgc caggcgctcc ccgatcaggc
     2281 gattgaacag ggtcgatttg ccgacgttcg gccggccgac aatggcgacg aggggtttat
     2341 tcacggtctc ctcgtcggca gcggtgtgac cgttggcgtc ggcgttccgc gcggcgcgcg
     2401 ctcgatgatg acctgcaccg tcgccggcag cacgctccgc gccacgacgc cctccggcgt
     2461 ctccacgcgc ggctcgatct gatgcgtgcc gatgcccaga tcggacagat cgagaatcac
     2521 gcgtacgtcc tgccccaggt cgatcgtctc gagcacgggc agcgcgccct cgaggatcac
     2581 gccgacggtc tcgggcgaaa cgcgcacggc tagccccggc tgcaatccga tcgcgatcgg
     2641 ttggctgggg atggtcaggc tgccgacgat cggctcgatc ttgacgacga cgcgcaccga
     2701 ctggcgatcg aacaacgcta cgtcgggcgg aaggacgaga ggcacgtcct tctcgatgtc
     2761 cgtctgcgcg ccggagatgt cgatcagctc cgtcgcgacg aacgcgggca gagattcgag
     2821 tgcattcgac gagccgatca acgtgaccgt cggcgggatc acgtcgacgc cggccagccg
     2881 gtagcctgac gcctgtgtgc cggtcatttc gacctttacg gagagattgc gaaagccggc
     2941 ctgctgctcg aagtcgaccg aaaggcgcac gcgatcaggt atcacctgga cgttcggcac
     3001 cacctgccca tcggcgccga ggaccttcag cgcaatcgtc tgtgaaaccg gctggcttaa
     3061 gccttgcacg ctgaactcgc tcgccacctt cgccacctgg ctcaccgacg aggcaggtcc
     3121 ggtcaccgtc gccgtcggcg gctgcgtcgc cacgctgtcg atcttatagg tccgagccgg
     3181 ctcgccgacc aggttcactt ccaccgaaaa ctgagcagaa tcgcgcggct cgaccttcgc
     3241 gctgaccgac ggcggctcga cgtgcacgat gcccaccggg cgcacgttcg agtcgagcac
     3301 gctcgcctgc acgggatacg attcccgtgg ctgcgacaga tcgactgtcg ccgtgatgac
     3361 tttgtcgttc aaatcggccc aggtttcccg cggcgcgctg actgtcacgt ccactgtcga
     3421 cgcgctgacg tcgtacacga ccgtgtccgg gggctggttg acgaaagcga tcgggatgag
     3481 ctcgaaagtg cgttcactgc tgggattcgc ctcgttgacg gcgaccaccc acacgatgac
     3541 ggcgaggatg acggacaaca agatgttcca cagattgcgc gcgagccaac ggagcatagg
     3601 attcaacgtc aaactccaaa tcctaaactt caaagcgtca acggtcgggg tttgagggcc
     3661 gcgtgggcga ctggcgccgg ccagacgtga ggcgcggcag gcgtaaacgc gacagccagc
     3721 gcgggcttgt ctccttaagc tccgactggt agaacgtgcg aagcacgtcc tccagccgcc
     3781 gaggctcgat acgccggatc aggcggccgt tgtgcgcgat cgacacgtgg ccgtgctctt
     3841 ccgagacgac gacggcgacc gcgtcggtcg actcggtaat gccgatcgcg gcgcggtggc
     3901 gcaaacccat gcgccgatcg ctcaggaagg ccgaagtcag cggaaggatg cacgccgctg
     3961 ccaggatacg atcctggcga atgatcgccg cgccgtcgtg cagcatcgtg cccgtgtaga
     4021 acaccgtctg cagcagctcc ggcgtgactt cggcgtccag ccgaacgccg gtgtcggcgt
     4081 aggattgcag gcctgtctcg cgctcgagga cgatcagggc gccctgacgc ctgtcggcca
     4141 ggcgcgtcgc cgtctgtgcc acggccgaga tgacggactc gattgacgcc acgtcacgcg
     4201 ccgtctggcc gaagcgcgga ccggcgcggc ccaggcgctc cagcgcgcgc cgcagctcgg
     4261 gctggaagat gacggggatg gccaccagca gggcgaaccc ggcgcggctc agcagccagc
     4321 ccagcgccgg gaaactgaag gcgctgccca acagtgcgcc gatgacgacg agcgtgagca
     4381 cgccacgcag cagcgtatcg gcctgcgtcc cgcgcaccag tgtcagcgcg ccgaagaaca
     4441 ccagcgccac gagggcgatg tccagcaggc tcccccactc gaggcgatcg agaacggcga
     4501 gcaggtcggc catgtcagcc cccaagctgc gcaaggagct gccggtattc ggcggcgttg
     4561 ggcgggttga gcgcgacaat ggcgcggatg gtggccatcg cctcgcgctt catgccggca
     4621 ttgatctgca gatcgcccag cgcgtcgagc tgctgaatac cttccgggat gtgctggttc
     4681 tcgatgcagg cctgagcgag ccgcgcgcgg agcgccatat ccgtcgggtg agcgcgcacc
     4741 tggtcgtcga ggatgggaat gactttgtcg gtcttgccgg aggacttgta cgccgccacc
     4801 agctcgtcga tttccttgat cgcgcgcagc ggttggttcg tcttgaagta caggtcgatc
     4861 aacgccacgt gcgcgcgctc gtcgccgggc gcgatcgact tgatctgctc gtagatcgcc
     4921 agcgcatgcc gccagtcgat gcgctgaacg tgaatgtcgc ccagcttgtg cagcacctgg
     4981 accgtccagt tgcgctgcgc cgatccgcgc ggcgccagct tgagcgcatc ctggtacacg
     5041 tcgcgcgcct tgttgtagtc ggcgagctgg taataagcgt cggccagttg caggtagtgc
     5101 tcgagcgcgc ggtcgatctg gccgtaggac accagcagct cgatgagctt gctgcgcacg
     5161 ttgacgtcca tcggcgccat cttgaggatg cgctgatagg tgccgatggc caggcgggct
     5221 tcgccgcgcg cgctgtacgt atcggcgacg gcgaggtatt tggccagggc agcctcggcg
     5281 cggccgcagc tccacaggat atccccgagt cgcaaatgca gcggcaaata gtacggcgcg
     5341 ccgaggatcg ccgtctccgc aatctccagg gccgaataga gcttctcgcg cttcgcgtat
     5401 tcgccgatca gcgccatcgc ttccagcatt tcggtgggat tgggaaccgt caacagctcg
     5461 gccagcgtaa tagccgggcc gccttcgatg atgagctggt cgagccgctt gcgcgccacg
     5521 ccaactttgt cctcccaacc cttgctcttg aggaactcga ccagcgcgtc ggcgaattgc
     5581 gtcgcctgct ccgaatcgcc cttggccgcg tacgattctg ccaggctctg gtacacctga
     5641 atcagatcgt ccgcctgctc gcgccgcacg gttcccaggt cgatgatctt gagcacttcg
     5701 aggaagtaag acagcgcctc gtcgacgtgg cctttcgcgc gatagcactc gcctagcgcg
     5761 aagaggctgc ccagccggta ctccggcgcg tcgacagtgc gctgaaattc ctggatagcc
     5821 tcgtcgaagt gcatggtctc ctgcaggagg agaccgaggt tgaagtgagc ggccggcatt
     5881 tgcgcgcccg ccttgatgat gcgctggtag gcgtcgatag ccgcctgcac ctcgccgctc
     5941 gtttgatagt cgatggcctg gccgaccagc gcgtcgatct cggccttgct caactgctgc
     6001 gtcggcggca cttcctcgaa gatcgagccg gccagctcgg atagtgcttt ctcacgcgcg
     6061 atttcgaccg ggctgccacg caactcttcc gcttcttctt cgatcggcaa atcgaagatg
     6121 gacgtatcga ccttgcgcgc aggaggcggc gcaggtggcg ccggctggcc gccgcgcagg
     6181 agctccatcg ccgtcaagat gtccggatcg tccggatcga gagtggccgc cgcttgcacc
     6241 tgctcgacgg cttcctgaaa ttgtccgcgt ttgttgtgaa tgcgcgccag cgccagatga
     6301 gcgcggatgg ccagcttggt cttgccttga tgccggtaca cgcgggccag acggcgctga
     6361 atgtcggtat tgttcggatc gagccgggct gcgcgcaccc agatgtcgac cgccgcgtcc
     6421 acgtcgttgc gccgcagacg cagatcggcg atggccgcgt acgcgcgggc cgcgccgtcg
     6481 agccggccga tgcgctccag cgcgtcggcg accttggacc acgtctcctc atcgtcggga
     6541 aactgggtca gcgccttttg atattcggcg atggcccgcg cccacgcttt cgcggcgacg
     6601 tggccggcgg cgcgctgcag cgcttgttgg taggcttggc gatctccagg catatcgatc
     6661 tcgctaaaca atccagaatg caggtcgtcg aacgaggccc gccagactca acgcctcttc
     6721 gattgcgccc ccggcttcga cctgcccttg ccggacggct tgaccttcgc cgcgcgcttc
     6781 aacccggcgg caggcttggc ccgcgcccca gtgggggacc cccttgcagc cgggatctcg
     6841 gacaatcgcg ctgcggcccg cgcgtgaatt tcccttgcac gctcgggcgc gagcagctca
     6901 agtaatatcg ccttctgggc gtgcatgcga ttctcggcct gctcccagag cgcggactga
     6961 ggcccgtcgg cgacttcgtc ggtgatctcc tgtcctcggt gcgcgggcag gcaatgcatg
     7021 acgatcgcgt tgcgccgcgc tctggcgagc agggctgcgt taatctggta tcgtggcgag
     7081 aaagcatcga ttcgcttttg cgtttcggct tcctggccca tgctcgtcca cacgtccgtg
     7141 tacagggcgt cggcctcgcg ggccgcttcg ttcgggtcgt gcgtgaagta aagatggctg
     7201 ccggacttgg cagcaaatgc ctgcgctttc gccacgacgt cgcccttgat ctcgtagccg
     7261 atcggcgtgg cgatggcaaa atcggcgcca accagcgtgc agccgaaaat cagggagttg
     7321 gcgacgttgt tcccatcgcc gatataggcg attttcatcc ccttgagctt gccgaagcgc
     7381 tcgtacaccg ttagcaggtc gccggctccc tggcaagggt ggctgtagtc gctcaatccg
     7441 ttgatcacag gcaccgaggc gtgccgggcc aggtcgagca cgtccttgtg gctgaacaca
     7501 cgggccatga tcgcgtcgac gtaacggctc aggacgcggg ccgcgtcgcc gaccgactcg
     7561 cgctggccga gggcgatctc gttcggcgac aggtacaaag cgtggccgcc caggtgcagc
     7621 atggctatgt cgaacgacac gcgtgtgcgc aggctgggct tctggaaaat catccccagc
     7681 gttttgccct gcaaatacgg cggatttccg ccctgtttcc actcggcctt gagcgccacc
     7741 gccaaatcca gcaggccgat cagctccttg gaagtcaagt cggccaacga gagaaagtgc
     7801 ttcatgcaga cactcctcga cggaatgtgc gtaggcgaac aaagtatagc acaaggaaag
     7861 acgagagtaa aatgaatttc aagcaagtat ttagctcact ctcattctgg tctaatgctt
     7921 cccgtttaga atttccaatc gctgtagccg ggctgactgg ccatccgtcg cgcccggaca
     7981 ggcctcttgt tcatggctgt cgagtttcca gcgatcgagg ctcgtggtac atttgttctt
     8041 ccacgggcag gcgctgtgat gaagcgacct gctggggaca actgctactt cttgtataat
     8101 ttacggtcgc acgaacctga cttgcggagg tcacatttat gatcgacgtg gtcgatttga
     8161 cgaagtccta cggcgagact cgggcgctgc gcggcgtgag cttccacgtc gccgaaggcg
     8221 agatcgtcgg attgctcggc ccgaacggcg cgggcaagac gacgctcatg aagatcctca
     8281 ccggctatct tcacccggac gaaggcgagg ccagcgtcga cggactcgac gtgctgacgc
     8341 acacgctcga agtgcaggca cgcatcggct atctgcccga gaatgcgccg ctgtaccctg
     8401 agctgtcgat tcaatcgtat ctcaggatga tggcggacct gcggcgtatc ccgcaggccg
     8461 accagttgga ccggatttcc gaggccgtgc atgcgaccag cctggccgac catctgactc
     8521 gcccgattgg cgagctgagc aagggctatc ggcagcgcgt cggcctggca caggcactcc
     8581 tccacaagcc gcgactgctc atcctcgacg agccgactat cggcctcgac ccaactcaga
     8641 tcatcgagat ccgcagtctc attcggcggc tcgccaggca cagcacgatc ctcttctcga
     8701 cgcacatcct gcccgaagtc gaagcgctgt gcgatcgggc gatcatcatc atgaacggcc
     8761 gcatccgcgc cgacgcgcgc ctctccgagc tggcgagtac atcagacgcc atcctcgtcc
     8821 tgcaagacga tgtgaaggga gtcgaaagcg cgttgcggga gatgagcggc gtgcgcggtg
     8881 tcgagccgat gaaggttttg gatggctatc cggccttccg tgtgctgggc gcggagcggg
     8941 tcgacctgtg ccccagcgtg tacgacgtgg cccgccaaaa cgcctggccg ctccgcgagc
     9001 tgcgccggga cgtgcgcaca ctggagaccg tcttcaatca actggcgacc acggccttcg
     9061 aggacgagga tgagccggaa gggttgtgac ccatgaagca aatctcctcc atcacgcgta
     9121 aagaactcga cagttatttc agctcgccga tggcgctgat cttcgtcggc gtgtttctgg
     9181 ctttcacgct aggcgtcttc ttctggggac ccgaagcctt cttcgcgcgc ggcatagccg
     9241 acgtacgccc cttattccgc tggatgcccg tgctgctgat cttcctcgtc gcggcactga
     9301 cgatgcgcca gtggagtgaa gaagagcgat cgggcacgct ggagatgctg ctgacgctgc
     9361 cggtgcgtcc ggcacaactg gtgctgggca agttcctggc ggtcatggca ctcatcgccg
     9421 tggcactcgc gctcacgctg ccgctcccgc tcagcatatc gctcctgggc aatctcgact
     9481 ggggaccggt gatcggcggc tacctcgcgg cgctgctcat ggcgggggca tacgccgcca
     9541 ttggcttgtt cgtctcgtca cgcacggaca accagatcgt cgcgctgatc gccaccgtgc
     9601 tgctgggcgg cctgttctac gtggcgggca ccggtttcat gaccgacttc gcgggcggcg
     9661 cgctcggcga cgcgctccgc gcgctcggca ccggcagccg cttcgagagc atcgagcgcg
     9721 gcgtcgtcga cgcgcgcgat ctcctctact atctgtccct gacggtcttc ttcatcgtgc
     9781 tcaacgtcgt gtcgctcgac agcaagcgtt ggggcgcagg cgagcggggg cgtgtccatc
     9841 ggcgggatat aggcctcacg gcggtgctcg tcgggctgaa tctggtcttg ctcaacacgt
     9901 ggagctacac gctgcaggga ttgcgcgtcg atctcacgca gcagcaggag ttcagcctat
     9961 cgcagacgac ccgggatctg ctcgccagcc tccaggagcc gctggtcatg cgcgcctaca
    10021 tcagcgacaa gacacacccg ctgctggagc cgctcaagcc gcgcatcgcc gacatgctgc
    10081 gcgagtacgc ggtcgcctcg gcaggaaaga tcacggcgga cgtcgtcgat cctgcgtccg
    10141 atccggcgat cgaagccgag gcgaaccaga cgtatggcat cgagccgacg cccctacaag
    10201 tgtccgggcg ctacgaagcg tcgctgatca actcgtattt cgacgtcctc gtgcgctacg
    10261 gcgaccagaa caccgtgctc aactttcgcg atctgatcga ggtgcagtcg aaccgcgacg
    10321 gcacgctcga cgtgcgcctg cgcaacctgg aatacgacct gacgcgatcg atcaagaaag
    10381 tgctgtacgg attccagagc gtcgatgcgg tgctcgccgc gatggaagag ccggtgcagc
    10441 tcacgctcgt cgtcacgccc gatacgctgc ccgaggcgct ccagtccgcg ccggagacca
    10501 ttcggaaagt cgcagaagac atcgcagcgc aatcgaatgg caagttcacg tttgaggaag
    10561 tgaacccgga cgaggcgggc agttctctta cgcgacaggc gctggtcgac acgtataagc
    10621 tgcagccgat ccccgtctcg cttttctccg aggattcgta ctacctcgac atgctgctgc
    10681 acattggaga tcagacgcga atcttgtcac ccggttcgga cgtgagtgat gcgggggtgc
    10741 gttccgcgat cgaatcggca ttgaagcgat cgtcgacggg cttcctcaag gtcgtcgggc
    10801 tgtggacgcc acccgccacg ccgacgacga atgtcctggg ccagccgcag cagccgatct
    10861 ccgactggca gctcatccgg cagcagctcg gccaggacta caccgtgcgc agcgtcgatc
    10921 tgtccagcgg ccaggcgccg ccggacgtcg acacgctcgt cgtgatcgcg ccgcagaacc
    10981 tgaccgatag agaacgcttc gcgatcgacc aatacctgat gcgcggcggc tcggtcatcg
    11041 tcgccgccag caactacgcc atcacgcccg atccattcgg cggcggcctc gcgctccaac
    11101 cgctcgagaa cggcctgcgc gagatgctgc agcactacgg cgtcgacgtc ggccagtctc
    11161 tcgtgctgga tccgcagaac gagccattcc cgacgtatgt cgcccgcaac gtgggcgggc
    11221 tggttgtgca ggagctgcag gcgatcaact atccgttctt cccggacgtg cgcgccgacg
    11281 gaatggatcc agaaagcccg atcgtcgcgc ggctgccggc ggtgacgatg aattgggcct
    11341 cgccggtcga gctggatgcc gagaagaacg ccaagcgcca gaggagcgtg ctgctgcgtt
    11401 caagcccgct cgcctggctg acgaccaaca ccaacatcca gccggacttc cagtcgtatc
    11461 ctgagctggg cttcccggtc gagggcgaac caaagtcata cccactggcg gtgtccgtcc
    11521 agggcgtgtt tcagagctat ttcaaaggca agccgtcgcc cttccagccg gaggcgggaa
    11581 gccctctcga cacgagcagc cagccgtcac tggagccgac gcctacgccg gccccggccc
    11641 aaccggtcag cgtgatcgat gagtcgcccg acacggcccg gctggtcgtg atcggcagcg
    11701 ccgagttcgt cgacgacgtc gtgctgcaga tctcgtcgaa cctgacgcgc gaccgctatc
    11761 tcaacaacct gcaattcctg cagaatgccg tcgactggtc ggtcgaggat atcgatctgc
    11821 tcggcatccg cgcgcgcggc acgctcaccc gggtgctcaa gccgatggaa cagcgcgagg
    11881 agctgacgtg ggagctgctc aactatggca ttgcgctgct ggcgctgatc ggcatctgga
    11941 tgatctggcg cgcccgccgt cgc
//
LOCUS       DBNHXB010000105         2976 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2368831, whole genome shotgun sequence.
ACCESSION   DBNHXB010000105 DBNHXB010000000
VERSION     DBNHXB010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2368831"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..1729)
                     /locus_tag="AC162G_04045"
     CDS             complement(239..1729)
                     /locus_tag="AC162G_04045"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HIR5075017.1"
                     /translation="MTKARDIYLLSPKMLSPEVIAVAFAKTSRSPESFRDIAAELTDE
                     KSAEFHEKWVVGYGHASVAEHAVLHVAFENVSRLAVECIESNRLASYTEKSTRYQVFE
                     RDGYYTPPKIAASKHAGLYRDTIHALFDAYFDSIEPVRQVIREQYPRKEGESEKKHEG
                     RIRSKWIDNCRFLLPSATLANLGMTANARVMEHAITKMLSHPLEEVRDIGAEVRRVAS
                     AEAPTLLKYADHSDYVAKLEAGSWKPALSAVEGSEVGSWRLEVGDLARPISNLQSPTS
                     NLHPLILVDYDRDAEAKFVAACLYLASGMSFADALSRARAMGAEERAQVVVGVLRERG
                     DFDIPLRELEHATYTFDCVMDNGAYFDVKRHRMMTQTPQALTVDLGYAIPRVIDEAGL
                     RDLYCRTLDQSIDAYRVIARDFPNEASYLVANAFNRRVLLTLNLREAFHFCRLRGAPN
                     GHFSYRRIAVRMVEILRETHPIFARHMRCESYPSSKEIEEEFFAQV"
     gene            complement(1747..2670)
                     /locus_tag="AC162G_04050"
     CDS             complement(1747..2670)
                     /locus_tag="AC162G_04050"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR5075018.1"
                     /translation="MRVIYSLSDARLDVDSVVTIGAFDGVHRGHRVVIDSVRRAAAGR
                     GRASVVVTFFPHPSVVLGRAEPFYLTSTEEKIAVLDAAGIDLLVVMPFTLETARTRAA
                     DYVSMLVEHLRMREVHVGYDFAFGYQREGTVDFLRRTGPERGFDVVAIDALTNDGETI
                     SSTGIRRALRAGDVALAAQWLGRPFRLSGMVVEGDKRGRAIGVPTANLDVWKDHAVPA
                     NGVYACWAWIGDAKFGAAANIGVRPTVADDSLPTVEAHLLDFDRDIYGTSVALDFVAR
                     LRDERRFPGLDALVKQIKTDVEQARKILGRG"
     gene            complement(2670..>2976)
                     /locus_tag="AC162G_04055"
     CDS             complement(2670..>2976)
                     /locus_tag="AC162G_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075019.1"
                     /translation="DDRSWPSLLRPLDAGLAAFPAVTIGESDAARARHGMSIPGTWTR
                     ASLAPTHASADLFRVYDPAGRVVGLMRWDAQRNELRPEKILTSAPSPHDPPGEKGDA"
BASE COUNT          520 a          974 c          935 g          547 t
ORIGIN      
        1 cggcgctccc gtggaccatc gggccgggcc agacgatggc cttccggctg gagttcaatc
       61 gtccgcccgg cttcgccgcc atcctgcgca ttgtcacgca gcggttcgag ttcagcgggc
      121 tgcgataagc agcggatgag cagcggataa acggatacca aagcccccgg tttcccgccg
      181 aaaccggggg ctttcttgcg catcgcacga taagcgatac gcgataggcg atacgcgctc
      241 acacttgggc gaagaactcc tcttcgatct cttttgacga cgggtagctc tcacaacgca
      301 tgtgccgcgc aaagatggga tgcgtctcgc gcagaatctc gaccatgcgc acggcgatgc
      361 gccggtagga gaagtggccg ttgggcgcgc cgcgcaggcg gcagaagtgg aaggcttcgc
      421 gcaggttcag cgtgagcagg acgcgccggt tgaacgcgtt ggcgaccagg tacgaggctt
      481 cgttcgggaa atcgcgcgcg atgacacggt acgcgtcgat cgattgatcg agcgtgcggc
      541 agtaaagatc ccgaaggccg gcttcgtcga tcacgcgcgg gatggcgtag cccagatcga
      601 ccgtcagcgc ctgcggcgtt tgcgtcatca tccggtgtcg cttcacgtcg aagtatgcgc
      661 cgttgtccat cacgcagtca aacgtgtacg tggcgtgctc cagctctcgc agcgggatgt
      721 cgaaatcgcc gcgctccctc agcacgccga cgacgacctg tgcgcgttcc tcggcgccca
      781 tcgcccgcgc gcgcgacagg gcgtctgcga agctcatccc gctcgcaaga tacaggcacg
      841 ctgccacgaa tttcgcctcg gcgtcgcggt cgtaatcaac gaggattaga ggatggagat
      901 tagaggttgg agattggaga ttggagattg gacgggccag gtctccaact tccaacctcc
      961 aacttccaac ttccgaccct tcgactgcgc tcagggcagg cttccaactt ccagcttcca
     1021 gcttcgccac gtaatcgctg tgatcggcat acttgagcag ggtaggcgcc tcggctgacg
     1081 cgacgcgcct gacttccgcg ccgatgtcgc gcacttcctc cagcgggtgt gagagcatct
     1141 tggtaatagc gtgctccatc acgcgcgcgt tggccgtcat gcccaggttg gcgagcgtcg
     1201 ccgacggcag caggaagcgg cagttgtcga tccacttgct gcgaatgcgg ccttcgtgct
     1261 tcttctcgct ctcgccttcc ttgcgcggat actgctcgcg tatcacctgc ctcaccggct
     1321 cgatgctgtc gaaataggcg tcgaacagcg cgtgaatcgt atcccggtac aggccggcgt
     1381 gcttcgacgc cgcgatcttc ggcggcgtgt agtacccgtc ccgttcgaac acctggtaac
     1441 gcgtcgactt ctcggtgtac gaggccagcc ggttgctctc gatgcactcg acggccagcc
     1501 gcgacacgtt ctcgaaggcg acgtgcagca cggcatgctc tgcgacggag gcgtgaccat
     1561 agccgacgac ccatttctcg tggaattctg ctgacttctc gtcggtcagc tcggccgcga
     1621 tgtcgcggaa ggattccggc gatcggctgg ttttggcgaa cgcgacggcg atcacttcag
     1681 gactgagcat tttggggctt agcaggtaaa tgtcgcgggc cttggtcatg tcggtattct
     1741 ccgtgttcat ccgcgtccca aaatcttcct ggcctgctcg acgtccgtct tgatttgctt
     1801 caccagggca tccaggccgg ggaaacggcg ctcgtcgcgc aagcgcgcta cgaagtcgag
     1861 cgcgacgctc gtaccgtaga tatcccggtc gaaatcgagc aggtgtgctt cgaccgtggg
     1921 aagtgaatcg tccgccaccg tcggtctcac gccgatgttg gcggctgcgc cgaatttcgc
     1981 atcgccgatc cacgcccagc acgcatacac accattggcc ggaacggcgt gatccttcca
     2041 gacgtcgagg ttggccgtcg gcacgccgat cgctcggcct cgcttgtccc cctcgaccac
     2101 catcccgctc agtcgaaacg gccggcccag ccattgggcg gccagcgcca cgtctcccgc
     2161 acgcagcgcc cgccggatgc ccgtgctgct gattgtctcg ccgtcgttgg tgagcgcatc
     2221 gatcgccacg acgtcgaagc cgcgctccgg ccctgtgcgc ctgaggaagt cgaccgtccc
     2281 ttcgcgctgg taaccgaatg caaagtcgta gccgacgtgc acttcgcgca tgcgcagatg
     2341 ctcgacgagc atcgacacgt agtcggcagc ccgcgtgcgc gccgtttcga gcgtgaacgg
     2401 catgacgacc agcagatcga tcccggcggc atccagcacg gcaatctttt cttcggtgga
     2461 ggtcagatag aacggctcgg cgcggcccag cacgacactg ggatgcggaa aaaacgtcac
     2521 gacgaccgaa gcgcgaccgc gcccggcagc cgcgcgccgc acgctgtcga tcacgacgcg
     2581 gtgcccgcga tggactccgt cgaaggcgcc gatcgtgacg acgctatcca cgtcgagccg
     2641 ggcgtccgaa agagagtaaa tgactcgcat caagcgtcac ccttctcgcc gggcggatcg
     2701 tgcggcgagg gcgcagacgt caggatcttc tcaggccgca gctcgttgcg ctgtgcatcc
     2761 caccgcatca gcccgacgac ccggcccgcc gggtcgtaga cgcgaaacag gtcggcgctg
     2821 gcatgcgtag gggcaaggct cgcccttgtc caggtccccg gaatcgacat gccgtggcgc
     2881 gctcgtgcgg catcgctctc gccaatcgtg accgccggaa aggcggccag ccctgcatcg
     2941 agcggccgga gcagcgacgg ccacgatcta tcgtcg
//
LOCUS       DBNHXB010000106        11344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1697529, whole genome shotgun sequence.
ACCESSION   DBNHXB010000106 DBNHXB010000000
VERSION     DBNHXB010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11344
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1697529"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..1468)
                     /locus_tag="AC162G_04060"
     CDS             complement(254..1468)
                     /locus_tag="AC162G_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR5075020.1"
                     /translation="MAAKTQTLQFKQAVNAPPSEVYRAFTNSTALREWLCHAAQVEPR
                     KGGRIHLWWNSGYYATGEFTALVPNKKIAFTWLGRGEPGATSVQVSIAEKRGAAVVTL
                     AHAGIGRGKAWARAIEEIGDGWQNSLANLQSILETGWDLRFTRRPMLGINVGVFDAGV
                     AAKLSVPVNEGILLDGVSEEMGAHAAGLKKGDVLVNIGGKKVTNWASLTGALARHTAG
                     DEVKVQFYRGREKNALTVRLSPRPIDEPPATAAELAEAVRKMYADIDAQLAAAFEGVS
                     EEEASHKPGADEWSAKDVLCHLVAGERDWHAWIGELIGSQERWSDDWAGNIALRHVGI
                     LAAFPTVPALLEELKRHEAETVGMLAALPPEFVAHKGSYVRMAYSLLQAPEHVAGHRD
                     QIRDAIASARKS"
     gene            complement(1531..2748)
                     /locus_tag="AC162G_04065"
     CDS             complement(1531..2748)
                     /locus_tag="AC162G_04065"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR5075021.1"
                     /translation="MASEPSALRLQRLVNAPPAETFRMFTHPVALRDWLCDAAQVDPQ
                     KGGRLYLWWGSGAHATGAYTTFEPGKRLAMEWHHSREPEPARVRIAFAAKDGGTQVTL
                     AHALGSGPKWRKVAPAVRKAWESALENLQSALETGIDLRVARLPRLGILIDDFNTEIA
                     ARLGVPVDKGIRLAGVADGAGAQAAGLQKDDVLVKLGGKKASDFPSLVAALQGRLAGD
                     VVPVVFYRGDRKMSAPMELSKRPQAEMPPRAELVDRVRKSYSEIDAGFAKLVEGVSED
                     QAGNRPSPDAWSVKDAIAHFILTERDLQSWFAQMVNDREIGESLEFRPNVEARVRAVR
                     EVHPALGALLDELKRSERETVAMLSALPAEFVARKHLYRRVTGWMLEVLPGHFYEEHL
                     GLMQAAIQSARQK"
     gene            2992..3198
                     /locus_tag="AC162G_04070"
     CDS             2992..3198
                     /locus_tag="AC162G_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075022.1"
                     /translation="MMEIIKWPFEALWRLVGLVFELTGRFLAILIGIVLIGVGALLTA
                     TVIGACVGVPLLAFGILLVVRGLF"
     gene            3405..6149
                     /locus_tag="AC162G_04075"
     CDS             3405..6149
                     /locus_tag="AC162G_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR5075023.1"
                     /translation="MYRVRRIILRFAAIWIIEGLSLGLTAAIAPGISITPATVRWLIL
                     PDVAVAVDPSGVIMSAALLLGLMNVSIRPVLLLLTLPINTATLGATTLIINALLLWLT
                     SWIVPGFEITSLVAAVAGTLVLAAVNTLITSFTTIDDDDSFFQGVVERISKHQRIKGA
                     AEPGRGIVMLELDGLSYDRIKRAIEKGTMPTVREMIDKDGYVLSPIDCGLPSQTSACQ
                     AGIMYGDNYDIPAFRWYDKDQGKLFVSNNAADAAAIDARFSSGRGLLRGGSSVGNLTS
                     GDAEKSLLTFSTLNTSDETVNRLRADDLRLFFINPYLFPRTVALTLWDILVELWQALR
                     QKLRNVQPRINRLEKAYPIIRGVTSVVLRDICTYVVSLDVIRGVPAIYTTYLAYDEVA
                     HHAGPDTPDAFMTLRQLDGQIRRVRDIIARKAGRPYDLFVLSDHGQSFGATFRQRYGY
                     ELKDFIKKHLAHGTTVAHTSGHDVGASFTGALVEELKNMNEQSVTSRVGKATVGRAQT
                     ALQRRVDASVPAAPAAMDADVLVFASGNLANVYFSLRSGKISIDELNAAHPRLVDTLV
                     EHEGVGFVVGHDDEGAPIVLGKQGARNLRTGTVTGADPLARYGDVDLRAKQLLRLAEF
                     PHAGDLIVNSTVYEGQAVNGDLTVAAFEEQIGSHGGLGGQQTDAFLLHPGDMQPPSTS
                     NSTDIFAILNTRRGLPGTADRPQPQPAPAIDAWTPTHLWKGIRDWRTWTVRALHAALL
                     SRSAYHDIADDPYATGPALVVLLLAFVIFWLANGLQATTPVQEHPVVSVAVFAAGWIV
                     ALFGIHGAARLLGGKGQLIQTFRVVAFAAILNIVALLGFIPAAGPVFRIAALLLRFFA
                     AWIGLQEAHHLQGGRAFLLPIVAILVLAVSIVALRAMAGAAVFTLEAILDLLGIVPEV
                     "
     gene            complement(6208..7023)
                     /locus_tag="AC162G_04080"
     CDS             complement(6208..7023)
                     /locus_tag="AC162G_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5075024.1"
                     /translation="MIRGTQITYIGHATILIEMDGARVLTDPLLRDRIGHLRRHGLRL
                     GIPLRERIRRLNPNNRPAETLWYHSVDAVLVSHLHWDHFDLPSLRMLGYGVRLIVPQG
                     AAKQLRRRGFRHVEEVRPGDTFEMGSTTVEVTPAAHNGFRPPFGPVAPCLGYILHGSH
                     RVYFPGDTDVFPQMADLGKDLDLAFLPIWGWGPSLGNGHMDPHRAAEALALLRPRVAV
                     PIHWGTLYPLGLGMMRAGFITLPPYAFAHHAARLAPEVEVKIVEPGERLQLDG"
     gene            complement(7215..7898)
                     /locus_tag="AC162G_04085"
     CDS             complement(7215..7898)
                     /locus_tag="AC162G_04085"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR5075025.1"
                     /translation="MVILLYALVGFVLGAPINALADALPERRAATRPAYLARRAPPRS
                     RDVAVHLATAALFAILWARFERAGMIQLVLVSFYTLVLLLVTVTDLEHKLIPNRAIMP
                     ALAIAALASPLRFGERWPLALLGGAIGFVFFYAAFWIGERTIGRGALGFGDVKLATFV
                     GLISGFPQILVALVAGVFAGGALSLLLLITRRATLRTAIPYGPFIAIGGFYAMVWGGE
                     VILWYASRF"
     gene            7947..8777
                     /locus_tag="AC162G_04090"
     CDS             7947..8777
                     /locus_tag="AC162G_04090"
                     /inference="COORDINATES: protein motif:HMM:NF040134.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5656 family protein"
                     /protein_id="HIR5075026.1"
                     /translation="MLERVPAINRDRLSVLIGLILLALALIPLVSAPVRTVGASVLGS
                     ELGIPITPGGLITLLAAALACAGVETLIQPHPRVRSGQARPTFVFWILPALTVVAASQ
                     WLNRASAGWGWALQLLASGVALWLIMRAEYGTIDPDAAAAGRWRLMLNVLAYLLAFGL
                     FTMIWETRARSLIAATLMAVAAFLTSIDLMWVTRAGARRVLTHGAVVALIIGECAWAL
                     NYWRATPATAGLALVLIFYAFSGVAAQHLFGKLTQRVLIEFAVVIAAAILLLILGTPG
                     "
     gene            complement(9088..10485)
                     /locus_tag="AC162G_04095"
     CDS             complement(9088..10485)
                     /locus_tag="AC162G_04095"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5075027.1"
                     /translation="MRLRVGLIAIAIIVLAVILAAGSASVDSEVATIVARGDAYRDAY
                     LYSIASDQYLQALERQPGNPAILVRLCDVSQRLGRRDDAATYADRAEGAGASRAEVAR
                     CRARNAESSGQASRAAEQWSIAVAEQPEDRNAQMSLIGALVAAHDWQAAAAQAQAMLD
                     ADPGDAAALFFRGALLALDDPLRARPYLRQAGTPESLALADALDDPLGLSDRGYRAVS
                     IGRVFLEHGRLPLAWRAFIAATAGNPGYADAFAYLGITYDRLGDEALAGAFLDRALEL
                     DPSSPVGPYLRGVFLSRRARWAEARADLERALQFDPANPAIAFALGRALVELGHFAEA
                     DDHFVRALASEPDDFGWRLALAELYIGRLIRVTDRGLPAARRAVELNPGSAEARAWLG
                     WGLHLTGEHGQAEAELRTAISLDPALARARLYLGNVLVNVGRFEEGRTELLRAVDLDP
                     HGEIGARARQLLGDR"
     gene            10636..10821
                     /locus_tag="AC162G_04100"
     CDS             10636..10821
                     /locus_tag="AC162G_04100"
                     /inference="COORDINATES: protein motif:HMM:NF037100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR5075028.1"
                     /translation="MIGAVELPEIYEQDIRRAVEILKGAGCTHVYLFGSLRTGKVRDE
                     SDIDLAVRGCPKGEFTC"
BASE COUNT         2006 a         3750 c         3598 g         1990 t
ORIGIN      
        1 ccgatcgcag cactgtgtca tgccgaacgc ggcgcaccgg ggtagagatg cctcgtcggt
       61 cctcctcggc atgacacatc tggcgcggat cgcgtggtac gcccacgtgt gccagaccgg
      121 gtcgaaggat gtaaagtgta catgcaatag tgaaattggt gcaacaagag aacggccctc
      181 cgtcgatacg aaggaccgtt ccgctccggg tacgatttgg gatcaaaatc gtctccaatt
      241 ccaaaatcca gacttacgac ttccgcgccg acgcaatcgc gtcacggatc tggtcgcggt
      301 ggccggccac gtgctccggc gcttgaagga ggctgtacgc catcctgacg tagctgccct
      361 tgtgcgcgac gaactccggc ggcagcgccg ccagcatccc caccgtctcg gcttcgtgac
      421 gcttcagttc ttcgagcagt gccggcacgg tcgggaatgc cgccaggatg ccgacgtgcc
      481 gcagcgcgat gttgccggcc caatcgtccg accagcgttc ctggctgccg atcagctcgc
      541 caatccaggc gtgccagtcg cgctcgccgg cgaccaggtg gcacagcacg tctttcgcgc
      601 tccattcgtc agctccgggc ttgtgcgatg cctcctcctc ggacacaccc tcgaatgcgg
      661 cagccagttg ggcgtcgatg tcggcgtaca tcttgcgcac ggcttcggcc agttccgcag
      721 ccgtcgcggg cggctcgtcg atggggcgag gggagagcct caccgtgagt gcgttcttct
      781 cgcggccgcg atagaactgg accttcactt cgtcgccggc agtatgcctg gccagcgcgc
      841 ccgtcagcga ggcccagttg gtcaccttct ttccgccgat gttcaccaga acgtcgccct
      901 tcttcaggcc ggctgcgtgc gcgcccatct cttccgacac cccgtcgagg aggatgcctt
      961 cgttcacggg cacgctcaac ttcgccgcga cgcccgcgtc gaatacgccg acgttgatgc
     1021 cgagcatcgg ccgccgggtg aagcgcaaat cccatccggt ctccagaatc gattgcagat
     1081 tcgccaggct attttgccag ccgtcgccta tctcttcgat ggccctggcc cacgccttgc
     1141 cgcgtccaat gcccgcgtgc gccagcgtga cgacggcagc gccccgtttc tcggcgatcg
     1201 agacctgcac gctcgtcgcg cctggctcgc cgcgccccag ccaggtgaac gcgatcttct
     1261 tgttcgggac gagcgcagtg aactcgcccg tcgcatagta gccgctgttc caccacaagt
     1321 ggatccgccc tcccttgcgc ggttcgactt gcgcggcgtg gcacagccac tcgcgcaagg
     1381 cggtcgagtt ggtgaaggcg cggtacacct cggaaggcgg cgcgttgacc gcctgcttga
     1441 actggagcgt ttgagtcttg gcagccatgt gtgtcctcct gtcacacgga tttctcacgg
     1501 attacacgga tgggcaagta tccggacgac ttatttctgt cgcgccgact gaatggcggc
     1561 ctgcatgagg ccgagatgct cttcgtagaa gtggccgggc aggacttcga gcatccaccc
     1621 cgtcacgcgc cgatagagat gcttgcgcgc gacgaactcc gccggcagcg cgctcagcat
     1681 cgcaaccgtc tctcgctcgc tgcgcttcag ctcgtcgagc agcgcaccca gcgccggatg
     1741 aacttcccgg acggcgcgca cgcgcgcctc gacgttaggc cgaaactcca gcgactcacc
     1801 gatctcacgg tcgttgacca tttgcgcgaa ccacgactgc agatcgcgct cggtgaggat
     1861 gaagtgcgcg atggcatcct tgacgctcca ggcgtcgggc gaaggccggt tgccggcctg
     1921 gtcctccgag acgccttcga ccagcttcgc aaagcccgca tcgatctcgg agtaactctt
     1981 gcgcacccga tcgaccagct ctgcgcgcgg cggcatttct gcctgcgggc gcttcgacag
     2041 ttccatcggc gcgctcatct tccggtcgcc acggtaaaac acgacaggga cgacatcacc
     2101 ggccagccgg ccctgcagcg cggcgaccag cgatggaaag tcgctcgcct tcttgccgcc
     2161 cagtttgacg aggacgtcgt ctttttgcag cccggcggcc tgcgcgcccg cgccgtcggc
     2221 gacgcccgcc aggcgaatac ccttgtcgac cggcacgccg agccgggcgg cgatctcggt
     2281 attgaagtcg tcgatgagga ttccgaggcg cggcaggcgg gcgacgcgca ggtcgatgcc
     2341 ggtctccaac gccgattgca gattctccag cgcgctttcc cacgccttcc gcacggccgg
     2401 ggcgaccttg cgccacttcg gacctgatcc gagcgcatgg gcgagcgtca cttgcgtgcc
     2461 gccatctttc gccgcgaacg cgatccggac gcgggcgggt tccggctcgc ggctgtgatg
     2521 ccactccatc gccagccgct ttcccggctc gaaggtcgtg tacgcgcccg tcgcatgcgc
     2581 gccggagccc caccacaggt acaggcggcc gcccttctgt ggatcgactt gcgcggcgtc
     2641 acacagccag tcacgcagcg cgaccggatg tgtgaacatg cggaatgtct cggcgggcgg
     2701 cgcattcacg agtcgttgga gtctcaaagc ggaaggctcg gatgccatgc gaatctcctg
     2761 tgacacggat ttcccacgga ttacacggac gggcaagtac tgcaatctat tttaacatgc
     2821 ccggcaaatt gcgccatccc ccttggctta gacctggcag gtctcggaga cctgccaggt
     2881 cttcccatcc acccaaagcc cgaccaggga atccatcctc aggtcgttca cgggacgagc
     2941 tggcgcttgt atactcagcg cgactgaacc tgagacgaag gagaacgcag catgatggaa
     3001 atcattaaat ggcctttcga ggcattgtgg cggctggtcg ggcttgtatt cgaattgacc
     3061 gggcgcttcc tggcgatcct gatcgggatc gtcctgatcg gcgtcggtgc gctgctgacg
     3121 gccactgtga ttggcgcgtg cgtcggcgtg ccgctgctgg ccttcggcat tcttctggtc
     3181 gtgcgcggcc tgttctagcc acacggattg caccacacgg atttcccacg gattgcacgg
     3241 atgcaggcac gtcggccatg acggttggac tgccttgcgg ttttcccacg gattgcacgg
     3301 atgggcaggg aaagatttgc ccgtcgctta ccggcgcact ataatagtag gcgcactcca
     3361 catcacattg cttcgcacgc aacgctttgc gtgggaggag cctcatgtat cgcgtgcgac
     3421 gcatcatctt gcggttcgct gcgatctgga taatcgaagg gctgtccctg ggcttgacgg
     3481 cggcgatcgc cccggggatc agcattactc cggcgaccgt ccgctggctc attcttcccg
     3541 atgtcgccgt ggccgtcgac ccatccggcg tcatcatgtc ggcggcgctg cttctggggc
     3601 tgatgaacgt gtcgattcgc cccgttctgc tcctgttgac gctgccgatc aacacggcca
     3661 ctctcggcgc gacgacgctg atcatcaacg cgctgctgct ctggctcacg tcgtggatcg
     3721 tgccggggtt cgagatcaca tcgctggtcg cggccgtcgc cggcacgctt gtgctggcgg
     3781 ccgtcaacac gctgatcacc agcttcacca caatcgacga cgacgactcc ttctttcagg
     3841 gcgtggtcga gcgcatcagc aagcaccaaa gaatcaaggg ggcggcggag ccgggccgtg
     3901 gcatcgtcat gctcgagctc gacggactga gctacgaccg catcaagcgc gcgatcgaga
     3961 agggcaccat gccgaccgtg cgcgaaatga tcgacaagga cggctacgtc ctgtcgccga
     4021 tcgactgcgg attgccctcc cagacctcgg cctgccaggc tggcatcatg tacggcgaca
     4081 attacgacat tcctgccttc cgctggtacg acaaggatca gggcaagctc ttcgtctcca
     4141 acaacgccgc cgacgcagcc gccatcgatg cgcgtttctc gagcggccgg ggcttgctgc
     4201 gcggcggatc gagcgttggc aatctgactt ccggcgacgc cgagaagtcg cttttgacct
     4261 tcagcacgtt gaacaccagc gacgagacgg tgaaccggct gcgcgcggat gacctgcgcc
     4321 tgttcttcat caatccttac ttgtttccgc gcaccgtcgc gctgaccttg tgggatatcc
     4381 tcgtcgagct gtggcaggcc ctgcgccaga agctacgcaa cgtgcagccg cgcatcaacc
     4441 gcctggaaaa ggcttatccg atcatacgcg gcgtgacgag cgtcgtgctg cgggacatct
     4501 gcacgtacgt ggtctcgctc gacgtgatcc gcggcgtgcc ggcgatctac acgacctacc
     4561 tggcctacga cgaagtggct catcacgccg gaccggatac gccagacgcg ttcatgacgc
     4621 tgcgccagtt ggacggtcag attcgccgcg tgcgcgacat catcgcccgc aaggcaggcc
     4681 ggccgtacga cctcttcgtc ctctccgatc acggccagtc gttcggcgca accttccgcc
     4741 agcggtacgg ctacgagctc aaagacttca tcaagaaaca cctcgcgcac ggcaccaccg
     4801 tcgctcatac gagtgggcac gacgtcggcg cgagcttcac cggggcgctg gtcgaagaac
     4861 tgaagaatat gaatgagcaa agcgtgacca gtcgcgtcgg aaaggcgacg gtcggccgcg
     4921 cgcaaacggc tttgcagcgc cgcgtggacg ccagcgtgcc cgctgcgccg gcagcgatgg
     4981 acgccgacgt gctcgtattc gccagcggga acctggcgaa cgtgtacttc agcctgcgca
     5041 gcggcaagat cagtatagac gagttgaacg ccgcgcatcc gaggctcgtc gatacgctgg
     5101 tcgagcacga aggcgtcgga tttgtggtgg ggcatgatga cgagggcgct ccgatcgtgc
     5161 tgggcaaaca gggcgcgcgc aatctgcgca cgggcacggt caccggcgcc gacccgctgg
     5221 cgcgctacgg cgacgtcgat ctgcgcgcta agcaactgct gcggctggcg gagtttcctc
     5281 acgcaggcga tttgatcgtc aacagcacgg tctatgaggg acaggcggtc aatggggacc
     5341 tgacggttgc cgccttcgag gagcagatcg gttctcacgg cggtctcggc gggcagcaga
     5401 cggacgcctt cctgctgcat ccgggagaca tgcaaccgcc gagcacgtcg aactcgacgg
     5461 acatcttcgc gattttgaac acgcggcggg ggttgccggg cacagccgac aggcctcaac
     5521 cacagcccgc gcctgccatc gatgcctgga cgcccaccca tctctggaag ggcatccgcg
     5581 actggcgcac gtggaccgtg cgcgctctgc acgccgcgct cttgagccga agcgcgtatc
     5641 acgacatcgc ggacgacccc tacgccaccg gcccggcgct cgtcgtgctg ctgctcgcgt
     5701 tcgtcatctt ctggctggcc aacgggctgc aggcaaccac gcccgtgcag gaacaccccg
     5761 tggtgtccgt cgccgtattt gccgccggct ggatcgtcgc cctgttcggc attcacggcg
     5821 ccgcccgatt gctaggcggc aagggccagt tgatccagac cttccgcgtc gtggccttcg
     5881 ccgcgatcct gaacatcgtc gccctgctcg gcttcattcc ggctgccggg ccggtgttcc
     5941 ggatcgccgc cttgctgctg cgcttcttcg ccgcgtggat cggcctgcag gaagcgcacc
     6001 acttgcaagg aggtcgcgcc ttcctcttgc ccatcgtcgc cattctggta ttggctgtct
     6061 cgatcgtcgc gctgcgcgcc atggcaggcg ccgcggtgtt cacactcgag gccattctgg
     6121 acctgttggg catcgtgccc gaggtgtgat cgggcgtggt cagagacggc gctatggaat
     6181 ctgtcaggaa accgctaaag gagagcgtca gccgtcgagc tggaggcgct cgccaggctc
     6241 gacgattttg acctcgacct ctggcgcgag cctcgccgcg tgatgcgcga aggcatacgg
     6301 cggcagcgta atgaagccgg cgcgcatcat ccccagcccg agcggataga gcgttcccca
     6361 gtggatgggg acggcaacgc gcgggcggag cagtgcgaga gcctcggcgg cgcggtgcgg
     6421 gtccatgtga ccgttgccca gcgaaggccc ccagccccag atcggcaaga aggccaggtc
     6481 gaggtccttc cccagatcgg ccatctgtgg gaagacgtcg gtgtcgccgg ggaaatagac
     6541 gcgatggctg ccgtgcagga tgtagcccag gcagggagcg accgggccaa acggtgggcg
     6601 aaatccgttg tgcgcggccg gcgtcacctc caccgtcgtc gatcccatct cgaaggtatc
     6661 gcccgggcgg acctcctcca cgtgcctgaa cccccgccgc ctgagctgct tcgccgcacc
     6721 ctgcggcacg atcaggcgca cgccatagcc gagcatgcgc agcgacggca ggtcgaagtg
     6781 atcccagtgc aagtgagaga ccaggacggc gtcgacggag tgataccaga gcgtctccgc
     6841 cggccggttg ttcgggttga ggcggcggat gcgctcgcgc agggggattc cgaggcgcag
     6901 gccgtgccgg cggagatgcc cgatgcgatc gcgcaggagg ggatcggtca gcacgcgcgc
     6961 gccgtccatt tcgatcagaa tggtggcgtg cccgatgtaa gtgatctgcg tgccccgtat
     7021 catgcagctc gcgcctctaa atcacccggc ccgatggagt tgcggcatta tagcataggt
     7081 cgatcgcggg gcgttttgac ctcaccccca gcccctctcc tgacaacgaa aagactgttg
     7141 tcaggagagg gaagcgaggc agcggataat gccactcaca tgcgtttatc cgttgtatat
     7201 ccgctgccag cctgtcaaaa cctcgacgcg taccagagga tcacctcgcc gccccacacc
     7261 atcgcgtaga acccgccgat ggcgatgaac ggcccgtaag gaatcgccgt gcgcagcgtc
     7321 gcacgtcgtg tgatgagcag cagcagactg agggcgccgc cggcgaacac gccggcgacg
     7381 agtgcgacaa ggatctgcgg gaagccgctg atgagcccca caaacgtggc gagtttgacg
     7441 tcgccgaagc cgagcgcgcc tcgcccgatt gtgcgctcgc cgatccagaa ggccgcgtaa
     7501 aaaaacacga agccgatcgc gccgcccagc aaggcgagcg gccagcgctc tccaaagcgc
     7561 agaggcgacg cgagcgcggc gatcgcgagc gcaggcatga ttgcgcgatt ggggatgagc
     7621 ttgtgttcca ggtcggtgac ggtaacgagg agcaagacca gcgtatagaa ggacacgagg
     7681 acgagctgga tcatccccgc tctctcgaag cgcgcccaga ggatagcaaa cagcgccgcc
     7741 gtcgccaggt gcacggcgac gtcgcgcgag cgcggcggcg cgcgccgcgc caggtaggcc
     7801 ggcctcgtgg ccgctcgacg ttccggcagc gcgtcggcca gcgcgttgat cggcgcgccg
     7861 aggacaaatc caaccagcgc gtaaagaagg atcaccatcc ctccgtcgtt gacaaatgat
     7921 ctcctcgacg gtagaatcgc gccgccatgc tcgaaagagt ccctgccatc aaccgcgatc
     7981 ggctcagcgt gctcatcggg ttgatcttgc tggcgctggc gctcataccg ctcgtgagcg
     8041 cgccggttcg caccgtcggc gcttcggtgc tcggctccga gctgggcatc ccgatcacgc
     8101 ccggcggtct gatcacgctc ctggccgccg cactggcgtg cgccggcgtc gagacgctga
     8161 tccaacctca tccgcgcgtg cgatctggcc aggcgcggcc gacgttcgtg ttctggatcc
     8221 tgcccgcgct caccgtcgtc gccgcatcgc agtggctgaa tcgcgcgtcg gccggctggg
     8281 gctgggcgct tcaactgctg gcatccggcg tggcgctgtg gctcatcatg cgcgccgagt
     8341 acggcacgat cgacccggat gcagccgccg cggggcgctg gcgcttgatg ctcaacgtcc
     8401 tcgcctacct cctcgccttc ggcctgttca cgatgatttg ggagacgcgg gcgcgcagcc
     8461 tgatcgccgc cacgctgatg gcggtcgccg cattcctgac ctcgatcgat ctgatgtggg
     8521 tcacgcgcgc cggcgcgcgg cgcgtgctca cacacggcgc agtcgtcgcg ctgatcatcg
     8581 gcgagtgcgc ctgggcgctg aactactgga gagcgacgcc cgccacggcg ggcctggcgc
     8641 tggtgctgat cttctatgcg ttcagcggtg ttgccgccca gcatttattc ggcaagctga
     8701 cgcagcgcgt gctgatcgag ttcgccgtcg tgatcgcggc ggccatcctg ctgctcatcc
     8761 tcggcacgcc gggttagtca gaattagata aggcagcaga ggcggctcct tgcgaagcac
     8821 cccgcaacgc agcagagtgg gcatgccgcc gggcgtggga gcccggcccg ctaaccaaac
     8881 gatgggagcc cggcccgctc actaaacggt attgctcctg gccggcgtca ctgcggcggc
     8941 ggcagcaggc tgagcaccag gctaaggacg atcagcaagg agaaaatgat cagcacgatg
     9001 cgtatggtgt tctgcgtgcg ttgctgcgac gaagatcgct tggccatcct tcactccacg
     9061 acatgaaaat cgagttgatt atactaccta cctgtcgccc agcaattgtc gcgcgcgcgc
     9121 gccgatttca ccgtgcggat cgagatcgac ggcgcgcaga agctccgtgc ggccttcctc
     9181 gaaacggccc acgttgacca ggacattccc caggtacagc cgcgcgcgcg ccagcgccgg
     9241 gtcgagactg atcgccgtgc gcagctcggc ttccgcctgc ccatgctccc cggtcagatg
     9301 cagtccccac ccgagccagg cgcgcgcctc ggcgctgccc ggattcagtt cgacggcccg
     9361 ccgcgcagcg ggcagacccc tgtcggtgac acggatcagg cggccgatat acagctccgc
     9421 cagcgccagg cgccagccga agtcgtccgg ctcgctcgcc agcgcccgta cgaagtgatc
     9481 atctgcctct gcgaagtggc cgagttcgac cagcgcccgc cccagcgcga acgcaatcgc
     9541 gggattggcc ggatcgaatt gcagcgcacg ctcgagatcg gcccgcgcct cggcccacct
     9601 cgcgcgccgc gacaaaaaga caccgcgaag gtatggaccg acgggcgagc ttggatcgag
     9661 ctcgagcgcc cggtcgagaa aagcgccggc cagcgcctcg tcgcccaaac ggtcatacgt
     9721 gatgccgaga taggcaaacg cgtcggcgta gccggggttg cccgccgtcg ccgcgatgaa
     9781 cgcgcgccac gcgagaggca gccgtccgtg ctccaggaac acacgaccga tcgacacggc
     9841 gcggtatccc cggtcgctca gcccgagcgg gtcgtcgagc gcatcggcga gcgccaggga
     9901 ttccggcgtg cctgcctgcc gcaggtaggg ccgcgcccgc agtggatcgt ccagcgcgag
     9961 cagcgcgcca cggaagaaca gcgccgccgc atcgccggga tcggcgtcga gcatcgcctg
    10021 cgcctgcgcg gcggcggcct gccagtcgtg agccgccacg agcgcgccga tcaagctcat
    10081 ctgcgcattg cgatcttcgg gctgctccgc gacggcgatc gaccactgct ccgccgctcg
    10141 actcgcctgt ccgctcgatt cggcattgcg cgcccggcag cgcgccacct cggcgcggct
    10201 cgcacccgcg ccttcggccc gatcggcgta cgtcgccgcg tcatcccgtc ggccgagtcg
    10261 ttgggaaaca tcgcacagtc gcaccagaat cgccgggttg cctggctgcc gttcgagcgc
    10321 ctggagatac tggtcgctcg caatagaata gagataggcg tctcgatagg cgtcgccgcg
    10381 tgcgactatc gtcgccacct cggaatcgac gctggcggaa ccggcagcga ggataacggc
    10441 gaggactatg attgcgatcg cgataagccc aacacgcagc cgcatggggc ggattataac
    10501 agaggcttgc acccttcgcc gtcatctatg acacagagtt cccatcgaat cattttcaaa
    10561 ctcccgtcag ggcaggtata atagaatgga gttccaggat gccctggcga cacatgagct
    10621 gagggcgagg caacaatgat tggggcagtt gaacttcccg aaatctacga gcaggacatc
    10681 cggcgcgccg tggagatact caaaggcgcc ggatgcaccc acgtgtacct cttcggatcg
    10741 ctgaggacag gcaaagtacg tgacgaatca gatattgatc tggcagttcg cggatgtcca
    10801 aaaggagagt tcacctgtta ggcaggcttc tcaccgaatt ggatcatacc gtcgatctgg
    10861 ttgacctgga cagcgaagac ccgttcgcgc gataccttga agaaaaagag aggttgctcc
    10921 aagttgatgg agaagctggt gacgccgcta ccggagatat ggcagcaagt gaagcaagag
    10981 ctacaagcgt ttctggatag cctgcgcgtg aaataagggc agccgctact cattatcgca
    11041 tctgatccga aagcgtggca gcggtgaaag gacgctgagc tatgtctagt gtggacgatt
    11101 gcaacgaagg agcttcccga tcttcgttca cagattgaag acctcctgac gaaagacacc
    11161 gggaacgacg atggctgaca ctacccgatt gcgcacatcg tgcggtaatc tctagttacc
    11221 aatttccact gacaaggagg tagccacttg aacatcgtcg tctgcaccaa gcaagtcccc
    11281 gacaccgccg cgacgctcaa ggtcgacgaa cagtcgaacg tctcctgggg cgacgcgccg
    11341 ctgg
//
LOCUS       DBNHXB010000107         6953 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_356227, whole genome shotgun sequence.
ACCESSION   DBNHXB010000107 DBNHXB010000000
VERSION     DBNHXB010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_356227"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..2001
                     /locus_tag="AC162G_04105"
     CDS             313..2001
                     /locus_tag="AC162G_04105"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HIR5075029.1"
                     /translation="MKVRLIKLLVLSVVVMSLLLAPSVLAGPGGPSVKDGKDVKVTDD
                     NNNVDGGLANVTPSKDAQNRQSNEPTVAISGAASPVTGEVGDIIASGANDYRMVPYFG
                     DSWMPVYLSFDGGATWFGAAPFPNGYNTMVPGFPTDTSPEGLSSPIKDLDGSGDPVVR
                     FDAAGNLYIAGIAFNRNFDQPDRPVDNVVYVTKYNFTPGTEATASTTTTAGSPPHFTY
                     AGTTIVDRGAVGFAIPNQPFAFAGQFVDKEWMELDLNSPAASPCAGSVYVSFTSLHGV
                     GGSFPMKFSRSSDGGASFSQLSTISTGGQGGTVYTQGSDIAVASDGTIYVAYRTFTSN
                     SDQASIQVVKSTDCGSHWSQPVSTAAISAPQAPGVAFRTPTFAFVAVDNTDPDVVYVA
                     YQSFAGDYDIYVQRSIDGGATWGAPVQVNTDPGARHQIWPTIEVSNGALHVAWYDFRN
                     STTPANEALDVFYACTNCGGNAYPTFSSETRVTDKSHNGNCQMFGGGSVAFHGDYIEL
                     DARWDGENHVVHVAWTDNRDVPAKECDLTPGPGDTNSVGNRNQNIYADTLVVAP"
     gene            2149..5361
                     /locus_tag="AC162G_04110"
     CDS             2149..5361
                     /locus_tag="AC162G_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5075030.1"
                     /translation="MRQLMLLIVAAALVLALVPVTGAAFAGQGGDKLGKHDRELLAEA
                     RAQGETTVTLLIAALRGANRRVVQGIESLGGTVRFREDDIDYIRAIVPIDKVEAAARL
                     SGVEAVDLNEIIPLEDPRPDGIAPIVPQPPPGPGTPNNNPYMPIGDTGASQFMAANPT
                     WDGRGVTIGILDTGASLDHPSLLTTSTGERKVVDWVTFTDPLLRTGTSADPTWVDMQN
                     QVSGSTFTFITVTYTAPAAGSYRIGLFNERDFRLGGELGTDVNRDGNPTGSSGIFAVL
                     WDTASSTVWVDTNQNNNFADDMPMTDYKVNYDVNYFGVDNPATAVAERIPFVVQTDGK
                     LKFVNIGIVSGAHGSHVAGIAAGNSLFGGAMTGAAPGAKIVSVRVCLFITGCTSFALI
                     EGMIYAAKQSNVDVINMSIGGLPALNDGNNARALLYNRLIETYKVQMFISAGNSGPGL
                     NTVGDPSVATDVMSIGSSITKDGWQKNYGADAPEWDNLHHFTSRGPSEDGGFKPNVVA
                     PGHAISSTPLWQPGSGLPYTLPPGYSLFNGTSMAAPQAAGAAALLISAAKQVGAQYRP
                     DQLRQAMNSSARFLDEARFQAYDQGNGLIDVGAAWNLLKTNINTVDISSAVPVNTLLS
                     DFLATPGVGVGIHDREGVTAGVSYVRTYTFVRSSGGSKPIFYNLSWVGNDDDTFSSAS
                     SIALPLNTPVDLDVTINPATPGAHSAILNLDDPGTAGIDYQTMNVVVASGEFTASGGY
                     TVVKTGTLGRSQVPHYFFRIPASTPAFKVDFTGPNLITGTGQMRFLRWHPWGLGVDSN
                     AVSNCYSPPAPGGCFIGTTPVSPTSRTVTDPQAGVWEVTVDARRSSDSDNAAYTLTAS
                     ILGASVSPNPDVIPSATAGVSITRPYTITNLFGPFTGRAVGGGNLGSARRGVFSIAND
                     ELQVYPVVVITGSTSLRATIGSPSDPAADLDLFVYNCTSGTCVLAGQSADGDSEESVT
                     IGNPAAGNWEIRIDGFAVPAGTTTYNYVDVFVNPAFGSVSVTDADTLRATGAVWTVSG
                     SVVANAAPAAGRVLLGSVQVRTDTNVLVGSADVVIESVTP"
     gene            5605..6444
                     /locus_tag="AC162G_04115"
     CDS             5605..6444
                     /locus_tag="AC162G_04115"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HIR5075031.1"
                     /translation="MKNSPFSSRSRSTWIALTVAIALLAILAGSAAAVTDGVLDGEGH
                     PYVGLMVAQDANGNPLWRCSGTLLSPKLFLTAGHCTEAPAAHVEIWFDADVESGIPAN
                     GYPFDGDVGGTPYTHPNYNPNAFYLYDLGVVVLDKPVKMKTYGALPEQDVLDVMATER
                     GLQDVTFTAVGYGLQESFPDAASWKEHNVRVRMVAYPHLIQINGGLVGDFSLLLSNNH
                     ATGGTCFGDSGGPNFIDETNTIGGVTSFGLNGNCAGTGGVYRVDKADDLDWLYGTFGD
                     YLP"
     gene            6667..>6953
                     /locus_tag="AC162G_04120"
     CDS             6667..>6953
                     /locus_tag="AC162G_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075032.1"
                     /translation="MNERLWILMIVCLTACSSPPGTPDSQVSPMQLPQLPVTNYPLTG
                     TPDSQVSPLEAPVTASPLITPPSGTLSPAEKAVVDLAERLAIDPTSIQIERV"
BASE COUNT         1362 a         2275 c         2084 g         1232 t
ORIGIN      
        1 cgcaaatcat accatttcat acaaaatagc catatcctgt tgcatccggc tccggtataa
       61 tgaattggtc tgcttgtggt tcaacatgtt cacttctcta gcattgcgct cttgtcccgt
      121 cgagaccttc cgagcggaca tcctgactgg cgcagagcgt gcgtgtcaca cagactcgaa
      181 ctgagtgatt gaaaggaggt gataagagca ccgagcgatc acagaatcgg tgtgcacatc
      241 cgtaaaccgt gccgggttcc aggcaactgc ggcgtgttgt acctcaaccc aatcagcagg
      301 gaggaaagag acatgaaagt cagactgatc aagctgcttg tgctgtccgt ggtggtgatg
      361 agcctgctcc tcgcgccaag cgtgctggca ggcccaggcg gaccaagcgt caaagacggc
      421 aaagacgtca aggtgacgga cgacaacaat aacgtggacg gtggtttggc aaacgtgacg
      481 ccgagcaagg atgcccagaa ccgccagtcg aacgaaccta ccgtcgccat aagcggagcg
      541 gcctcacccg tcaccggcga agtaggcgat atcatcgcct ctggggctaa cgactaccgc
      601 atggtcccct acttcggcga ttcctggatg ccggtctacc tctccttcga cggcggggcg
      661 acctggttcg gagccgcccc attccccaac ggttacaaca cgatggttcc cggcttcccg
      721 accgacactt cgcctgaagg cttaagctcg ccgatcaagg acctcgatgg gtcgggcgac
      781 ccggtcgtgc gcttcgacgc ggcgggcaac ctgtacatcg caggcatcgc cttcaatcgc
      841 aacttcgacc agccggaccg gccggtcgat aacgtcgtct acgtcaccaa gtacaacttc
      901 acgcctggaa cggaagcgac agcgagcacc accacgaccg ccggctcgcc gccgcatttc
      961 acctatgccg gcacgacgat cgtcgaccgc ggcgcggtcg gcttcgccat ccccaaccag
     1021 ccgttcgcct tcgccggcca gttcgtcgac aaagagtgga tggagctcga tctgaactcg
     1081 cctgcggcca gcccgtgcgc cggcagtgtc tatgtgagct tcaccagtct ccacggagtc
     1141 ggcggctcgt tcccgatgaa gttcagccgc tcgagcgacg gtggggcaag cttttcccag
     1201 ttgagcacca tctctacggg aggtcagggc ggaacggttt acacgcaagg ctccgacatc
     1261 gccgtcgcat ccgatggcac catctacgtc gcctaccgca cgttcacgtc caactctgac
     1321 caagcctcga tccaggtcgt gaaatccaca gactgcggca gccattggag ccagccggtg
     1381 tcgactgcgg cgatctcggc gcctcaggcg cccggcgttg ccttccgcac gccgaccttc
     1441 gccttcgtcg ccgtggacaa cactgacccc gatgtcgtct acgtcgccta ccagagcttc
     1501 gccggcgact acgacatcta cgtccagcgc tcgatcgacg gcggcgcgac gtggggcgcg
     1561 ccggtgcagg tcaacacgga tccaggcgcc cggcatcaaa tctggccgac gatcgaggtg
     1621 tcgaacggcg cgctgcacgt cgcctggtac gacttccgga acagcacgac gcctgccaat
     1681 gaagcgctgg atgtcttcta cgcctgcacc aactgcggcg ggaacgccta tcccaccttc
     1741 agcagcgaga cccgcgtaac agacaagtct cacaacggca actgccagat gttcggtgga
     1801 ggcagcgtcg ccttccacgg cgactacatc gagctggatg cgcgctggga cggcgagaat
     1861 cacgtcgtcc atgttgcctg gaccgacaac cgcgacgtac cggccaagga gtgcgatctc
     1921 acgcccgggc cgggcgacac caacagcgtc ggcaatcgca accagaacat ctacgccgac
     1981 acactcgtcg tcgcgccata gcgtggatga cgagctaggc tcagcgccag agggcctgcg
     2041 tggagctgtg acggcctttc tgcgaaggag gtttatgtaa acgattacgc cagtttcgag
     2101 ttcccatcct tttagcagct ccatcactca ctccaatggg ggaaaaacat gcgtcaactt
     2161 atgcttctca tcgtcgctgc ggcgcttgtg ctggcgctcg tacccgtgac cggcgctgcc
     2221 ttcgccgggc agggcggcga taagctgggc aaacacgacc gcgaactgct cgccgaagcc
     2281 cgcgcgcagg gtgagacgac ggtcacgctg ttgatcgccg cgctgcgcgg cgccaacagg
     2341 cgtgtggtcc agggcatcga gagtctgggc ggcacggtgc gttttcgtga ggacgatatt
     2401 gactacattc gggcgatcgt cccaatcgac aaggtcgaag ccgccgcgcg cttgagcggc
     2461 gtcgaggccg tcgacctcaa cgagatcatc ccgctcgagg acccgcgtcc cgacggcatc
     2521 gcgccgattg tgccacagcc cccgcctggc cccggcacgc cgaacaacaa cccgtacatg
     2581 ccgatcggcg ataccggcgc gtcgcagttc atggccgcca acccgacatg ggacggccgc
     2641 ggcgtgacga tcggcatcct cgacacgggc gcctcgcttg accacccgag cctgctgacg
     2701 accagcacgg gcgagcgcaa ggttgtcgac tgggtcacgt ttaccgaccc cttgctccga
     2761 acgggaactt cagccgaccc gacctgggtc gatatgcaga accaggtcag cggcagcacg
     2821 ttcaccttca ttacagtgac ctacacggcg ccggcggccg gctcgtaccg catcggcttg
     2881 ttcaatgagc gcgatttccg gctcggcggc gagctgggca ccgatgtcaa ccgcgacggc
     2941 aacccgaccg gcagcagcgg catcttcgcc gtgctgtggg acactgccag cagcacggtc
     3001 tgggtcgaca cgaaccagaa caataacttc gccgacgaca tgccgatgac cgattacaag
     3061 gtcaactacg acgtgaatta cttcggcgtg gacaacccgg cgacggcggt ggccgagcgc
     3121 atccccttcg ttgtccagac cgacggcaag ctcaagttcg tcaacatcgg catcgtctcc
     3181 ggcgcgcacg gctcgcatgt ggcgggcatc gccgccggca acagcctgtt cggcggtgcg
     3241 atgaccggcg cagcgcccgg cgcgaagatc gtgtcagtgc gcgtttgcct gttcatcacg
     3301 ggctgcacgt cgttcgcctt gatcgagggc atgatctacg ccgccaagca gtcgaacgtc
     3361 gacgtcatca atatgtcgat cggcggcctg ccggcgctca acgacggcaa caatgcccgc
     3421 gccctgttgt acaatcgcct gatcgaaacg tacaaggtgc agatgttcat ctcggccggc
     3481 aacagtggcc cgggcctgaa taccgtcggc gatccgtcgg tcgccaccga cgtcatgagc
     3541 atcggctcct ctatcaccaa agacgggtgg cagaagaact acggcgcgga tgcgcctgag
     3601 tgggacaacc ttcaccactt cacgtcgcgc ggcccaagtg aagatggcgg cttcaagccg
     3661 aacgtcgtcg cgcccggcca cgccatctcg tccaccccgc tgtggcagcc cggcagcggc
     3721 ctgccataca cgctgccgcc cggctactcg ctgttcaacg gcacgtcgat ggctgcgccg
     3781 caggctgccg gtgcggcggc gctcctgatc agcgcggcga aacaggtcgg cgcgcagtac
     3841 aggccggatc agctccgcca ggcgatgaac tcgtcggctc gtttcctcga cgaggcgcgc
     3901 ttccaggcct acgatcaggg caatggcctg atcgacgtcg gcgcggcgtg gaatctccta
     3961 aagaccaaca tcaatacggt ggacatctcg tcggcggtgc cggtcaacac gctgctcagc
     4021 gacttcctgg cgacgccagg cgtcggcgtg ggcattcacg atcgcgaagg cgtcacggcc
     4081 ggcgtgtcct acgtccgcac ctacaccttc gtccgcagca gtggcggctc gaagccgatc
     4141 ttctacaacc tgagctgggt ggggaacgac gacgacacgt tcagctcggc gtcttcgatc
     4201 gcgctgccgc tgaacacgcc ggtcgatctt gacgtgacga tcaacccggc cacgcctggc
     4261 gcgcactcgg cgatcctcaa cctggacgat cccggcacgg ccggcatcga ctaccagacg
     4321 atgaacgtcg tcgtcgcgtc cggcgagttc acggccagcg gcggttatac cgtcgtcaag
     4381 acgggcacgc tcggccgcag ccaggtaccc cactacttct tccgcatccc ggctagcacg
     4441 ccggcgttca aggtcgactt caccggcccg aacttgataa ccggcacggg gcagatgcgc
     4501 ttcctgcgct ggcacccatg ggggctgggc gtcgactcca acgccgtcag caactgctac
     4561 agcccaccgg cgcccggcgg gtgcttcatc gggaccacgc ctgtcagccc caccagccgg
     4621 acggtgacgg acccgcaggc gggcgtgtgg gaagtgacgg tggatgcgcg gcgctcgtcc
     4681 gattcggata acgcggccta cacgctgacc gcctcgatcc tgggtgcgag cgtctcgccg
     4741 aaccctgacg ttatcccatc ggcgacggcc ggcgtgtcga tcactcgccc gtacacgatc
     4801 accaacctgt tcgggccgtt cacgggtcgc gccgtgggtg gcggcaatct cggcagcgcg
     4861 cggcgtgggg tcttcagcat cgccaacgat gagttgcagg tgtaccccgt ggtcgtgata
     4921 accggctcga cctcgctgcg cgcgacgatc ggcagcccgt ctgacccggc ggccgacctc
     4981 gacctgttcg tctataactg cacgagcggc acctgcgtgc tggccggcca gagcgccgac
     5041 ggcgactcgg aagagtcggt gaccatcggc aatccggcgg cgggcaactg ggaaatcagg
     5101 atcgacgggt tcgccgtccc ggccgggacg acgacgtata actacgtcga cgtcttcgtc
     5161 aacccggctt ttggctcggt gagcgtgacc gatgcggata cgcttcgcgc gaccggagcg
     5221 gtatggacgg tgtccggctc cgtcgtcgcc aacgccgcgc cggctgcggg tcgtgtcctg
     5281 ctgggcagcg tgcaggtgcg cacggacacg aacgtcctgg tcggatcggc cgatgtcgtg
     5341 atcgagagtg ttacgccgta gcaacaaatc tccgtcgcac agcgtgtgac gatctcgcca
     5401 cactcaaagc tcctccccat cggcgcacgt cgatggggag gagaaatgtt gccgcgaatc
     5461 cggcgatctg ggatcgtttt ccacatagat tttctaaccg caaaacatac caattctaac
     5521 aagatgcaca cgtgattatg cccacggctg gcgtataatg cgcatggatc gacgttcaat
     5581 tcaacatcaa aggagaaatc agacatgaag aactcaccgt tttcctcgcg aagcagatcg
     5641 acgtggatcg ccctcacagt cgcgatagcc cttctcgcga ttctggccgg ctctgcggcc
     5701 gccgtcacgg acggagtcct ggacggggag ggccatccct acgtggggct gatggtggcc
     5761 caagatgcca atggcaaccc cctatggcgc tgcagcggca cgctgctttc gcccaagctg
     5821 tttctgacgg ccggtcactg cacggaagcc cctgcggcgc atgtagagat ctggtttgac
     5881 gcggacgtcg aatcaggcat tcctgccaac ggctatccat tcgatggtga tgtggggggg
     5941 acgccgtaca cgcaccccaa ctacaacccc aacgccttct acctctatga cctgggcgtc
     6001 gtcgtgcttg ataagccggt caaaatgaag acgtacggcg cgctgcccga acaagatgtg
     6061 ttagacgtga tggcaacgga acgcggcttg caggatgtga cgtttacggc agtgggttat
     6121 ggactccagg aaagcttccc tgatgcagcc tcctggaagg aacacaacgt cagagtgcgt
     6181 atggtagcct atccccacct gatccagatc aacggtggcc tggtgggcga cttctccctg
     6241 ctgctgtcaa acaaccacgc gaccggaggc acctgcttcg gcgactcggg cggccccaac
     6301 ttcatcgacg aaacgaacac tatcggcgga gtcacctcct ttggcctcaa cggcaactgc
     6361 gccggcaccg gcggtgtgta tcgcgtggac aaggctgacg acctggactg gctttacggt
     6421 acattcggcg attacctgcc gtaagggcaa agcccagaca tccgccctcc agcatctgcc
     6481 atcgaactca gggatttcga atcgccttcg gaatccctga gttctcttaa cgtcgtcacc
     6541 acaaaatcgt atttgttacg aaataccgca gcggatgctt cgcgcaacgg gtaagcgata
     6601 gaccacctgt tcgccgtaag ggaacaatca gatatcaacg gcgtcttaag aacaggaggt
     6661 gcgctgatga acgagcggct ctggattttg atgatcgtgt gcctgacggc gtgcagtagc
     6721 ccgcctggta cgcctgatag ccaggtgtcg ccgatgcaac ttccacaatt accagttacc
     6781 aattacccac tgactggcac gcccgacagc caggtgtcgc cgctggaagc gccggtaacc
     6841 gctagccctc tgatcacacc gccgagtggc acgctcagcc cagccgagaa agcggtcgtg
     6901 gatttggccg agcggctagc gatcgatcca acctcgattc agattgaacg agt
//
LOCUS       DBNHXB010000108        16728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3208050, whole genome shotgun sequence.
ACCESSION   DBNHXB010000108 DBNHXB010000000
VERSION     DBNHXB010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 16728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16728
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3208050"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="AC162G_04125"
     CDS             complement(<1..208)
                     /locus_tag="AC162G_04125"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HIR5075033.1"
                     /translation="MSRILVATTNPGKVAEYQAILSDDGIQLLGLREVGVDFEVDESG
                     QTFEENARLKARVYATESGLLTLAD"
     gene            561..995
                     /locus_tag="AC162G_04130"
     CDS             561..995
                     /locus_tag="AC162G_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR5075034.1"
                     /translation="MTIDQGLSNLGPDLVVDFVQGGLTLTRTDRHILVQGSLTSALSA
                     ECVRCLESFRLPLQVQLEELFALIPGPHVLDPLYVVAPDGTIDLTYPLRENVLLAQPI
                     KPVCRPDCRGLCASCGKNLNEGPCDCEDELTDPRLAALKALL"
     gene            1011..2207
                     /locus_tag="AC162G_04135"
     CDS             1011..2207
                     /locus_tag="AC162G_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5075035.1"
                     /translation="MTTQGDDELDFLAELIEKGEALGYLTTGEILEAVPETEDRAEQL
                     ETVHQLLREAGIEIYEDSIRIGDESAPVEIEDLSEEADEETEDDEDFDLSGVASDDTV
                     GLYLKEMARVPLLTNQEEVALAKAVRRGRDAAKTLRRMRPSSQRRARLERYVNEARLA
                     REHLIKANTRLVVSIAKKYIGRGVPFLDLIQEGNLGLMKAVEKFDFTRGFRFSTYATW
                     WIRQTITRAIADQGRTIRVPVHMSDRIRHLYKTVRQLEQANGRQPTAEEIAEEMDIEP
                     RKVQWMMKVSWRPLSLELPVGEDEDAELGSFIEDETTPSPTQTVHERLLHEKVEEVLS
                     TLSPREARILRLRYGLVNGRSYTLEEVGRKFGLTRERIRQIQGKALRRLRHPRRSRVL
                     RDYLQQ"
     gene            2306..4846
                     /gene="mgtA"
                     /locus_tag="AC162G_04140"
     CDS             2306..4846
                     /gene="mgtA"
                     /locus_tag="AC162G_04140"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HIR5075036.1"
                     /translation="MPSQLVQLAAYWNTPADQLLKSLQATPDGLSTSEAQQRLKDWGP
                     NALKVRRRVTPWGLLLNQFKSPLVLILLFAVAVSAFVKEWTDAAVILTIVVGSALITF
                     SQEYSATNAVEKLRARLTYTVMGQRDGRPQSVPAEAIVPGDVVMLSAGSLIPADGVVL
                     DARDFFVNQAVLTGETFPVEKKPGTAPESASLTERTNCVFMGTTARSGAARVLIVQTG
                     AATAYGQIADRLSLRPPETEFERGIRHFGLMLTQVMVALILVVFAMNVISAKPPIDSL
                     LFAIALAVGISPELLPAIITINLSKGAREMAQHGVIVRQLNAIENFGSMDVLCTDKTG
                     TLTEGVVRLDGALDVRGNPSDEVFRYAYLNAHFQTGLSNPLDEAITAHHPLDLSDVAK
                     LGEVPYDFVRKRLSVVVGEGNQPPVFITKGALDNVLAVCRHIRGGEGVLPLDAAESER
                     IAQRFAQWSAQGFRVLGVAIKSLTPPSVYSRDDERDMIFVGFLLFFDPPKPDARLAIA
                     ALAKLGVRLKIITGDNRRVAVHVAEAVGMPVEGVLSGAELNVLRDEAVWHIVEHTDLF
                     VDVDPNQKERIIRALKKMGHVVGYLGDGINDAPALHDADVGISVEGAVDVAREAADFV
                     LLERGLDVLRRGIEEGRVTFANSLKYVFTTTSANFGNMFSMAGASLFLPFLPLLAKQI
                     LLNNFLSDFPAIAIAGDDVDRELVETPHRWDVKFIRNFMIVFGLVSSAFDYLTFGLLL
                     FIVHAAPEQFQTGWFVESLLTELLVMFVVRTRRPFYKSKPGRWLAISTLIVTLITLAI
                     PYLPFSAPLGFVPLPMEVMAFLLCITLLYLIATEVAKRIFYARVAVGA"
     gene            4878..5249
                     /locus_tag="AC162G_04145"
     CDS             4878..5249
                     /locus_tag="AC162G_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR5075037.1"
                     /translation="MLSIHLLGTPRIQRDGEPIYIIRRKSRALVYYLATHDKPLTREH
                     LVAFFWPDLERASVQQTLRTTQHGLRRLLGSALVVDEDTLAWRRIPRSILASSSHACS
                     LTPACDPRNRRVSFPGWVSGN"
     gene            complement(5536..6183)
                     /locus_tag="AC162G_04150"
     CDS             complement(5536..6183)
                     /locus_tag="AC162G_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HIR5075038.1"
                     /translation="MSSVTLAYVGLDESGSLSADTSLFTLVGVLTYQPDTIRYLIQRA
                     AVRSGKRLKRPRRAVSELKWHNTSRRLRADVLSRLARADIEIFALTVRKGGRKIEDTP
                     ENYAVLVCAVLQACWLAHPNVALAIDRRFTSPAQVAVLNTFLYRHWPTQGVLSISHVD
                     SQRNPLVQLADFVAGSVYAWHKEGDNTIKMIEGKISVALVEDWPEIKQRWTQKRK"
     gene            complement(6208..7755)
                     /locus_tag="AC162G_04155"
     CDS             complement(6208..7755)
                     /locus_tag="AC162G_04155"
                     /inference="COORDINATES: protein motif:HMM:NF019313.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KAP family P-loop NTPase fold protein"
                     /protein_id="HIR5075039.1"
                     /translation="MGKSFLIRMIREALENDKPADAPEFSFVEFNAWVYSGSDNLWAG
                     LVSHIYERIEQRLGSNATRSFRLDRKRRKQVRRLSFYAILYAIPIIAVTVLLSLDQIA
                     KIWADLPGLALLGVSSFLALIVGFVRTIKSIYDIVKKAIVERAEQLRQEAAQPDFRTK
                     IGFMADVKAEIDFVTELLAHQSKGKSIRVVIFIDGLDRCPPAKAVEVLEAIMLLLSED
                     NSPFYVFLGIDARVLVRAIEDRYGKTLVEAGISGYEYLDKIVQIPFTIPATDETGIKN
                     YIRALLPRPGEESARALVRPTAPQASTPQAETPPPAVEVSFTDDEQMAFDDFAKYISR
                     NPRRAKRIVNIYRLVRMLDKRSQSGLTAERAGQLIKWVILTEQWPYRMGLIISQRENA
                     GQLGEGIGSEDTDTLANLYTAVADKLHGEESRRLLYLDDDAELFTNFITHDPTQITAA
                     DICTFEPLTFNLNPAIGGEIRSMLARATAIVTQANEVDSKKDRSDPAYTGQDAMAGKT
                     ENERNRG"
     gene            complement(7782..8222)
                     /locus_tag="AC162G_04160"
     CDS             complement(7782..8222)
                     /locus_tag="AC162G_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015102.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HIR5075040.1"
                     /translation="MSDQPTAIPRKTPNEQKDAVSSWIPQEIYQTPIEQLDLSERVFN
                     SLKRTGITNVGEVLEMLASGEDAMLTIRNFGTKSYLELKEKLVAEGFLKEDEVLPGEA
                     PGAPIQIGSTFALNDRPRGDDRLGFTDYARAFAAMLSNPATQPR"
     gene            complement(8246..8376)
                     /gene="pstB"
                     /locus_tag="AC162G_04165"
                     /pseudo
     CDS             complement(8246..8376)
                     /gene="pstB"
                     /locus_tag="AC162G_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110708.1"
                     /note="ATP-binding protein; PstABCS is an ATP dependent
                     phosphate uptake system which is responsible for inorganic
                     phosphate uptake during phosphate starvation;
                     frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
     gene            complement(8377..9429)
                     /locus_tag="AC162G_04170"
     CDS             complement(8377..9429)
                     /locus_tag="AC162G_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075041.1"
                     /translation="MAPTPGISGASPPPDKAERGPGVWAVLAVLVILALVVFLALPFE
                     QMPVQPWAPQFQGQLRAALAQAGPYAIVVLLGAIVGLAEITSTFSNYPREALRTTWAR
                     FLIAVNAVAAGLAYAIVRASTPAADQLLTVIAVGIGFQALIRTKFTIAKQIGGSGNGD
                     VSLNLGWLYEQFQNLCKTQIDLELMKGRRTAVSRMIERYPSLAELHDIATYTILARAT
                     LTVEEEQARLADLDRLLDPKAPANFARTSMALMILENGGQGYVDLLLSQPVAKAPGEV
                     TQESVARRLIEKYTLKELIDFGLSLPASDEVHNWVRQAAEPAHDVNEASQKAAIAYFL
                     VQRVGAEALVKELEAK"
     gene            complement(9433..9714)
                     /locus_tag="AC162G_04175"
     CDS             complement(9433..9714)
                     /locus_tag="AC162G_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075042.1"
                     /translation="MDITRSDLNDEERKVLLALAKGGAMSPSQVSAETWILPGDALKL
                     LKTLADADLVVMRDDTDSADGKLVALTARARDMLDIGSARSMKQMKMKR"
     gene            complement(9808..10557)
                     /gene="pstB"
                     /locus_tag="AC162G_04180"
     CDS             complement(9808..10557)
                     /gene="pstB"
                     /locus_tag="AC162G_04180"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR5075043.1"
                     /translation="MNKIEVEHLNFYYDGNQALSEVTIGIPDRHITALIGPSGCGKST
                     FLRCLNRMNDTIPGARAEGRVLLDGVDVLSPKQDVVDLRRRVGMVFQRPNPFPQSIYD
                     NVTFGPRALRMKPASLDDLIEQSLRGAALWDEVKDNLKQSALELSLGEQQRLCIARVL
                     AVQPEVILMDEPCSALDPIATLKIEDLMQELKKNYTIVIVTHNMQQAARASDFTGFFW
                     LGELVEHGPTQEVFLRPKHRQTEEYITGRFG"
     gene            10736..11608
                     /locus_tag="AC162G_04185"
     CDS             10736..11608
                     /locus_tag="AC162G_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5075044.1"
                     /translation="MRPSTLCLMLCLTLLVGCAGRATAQDDAAGGAAIENIGSDTMVN
                     LALAWAEAYQGIYPDVRISVTGGGSGTGIASLINGTADIANASRAMKSEERAEAESNG
                     IQPVEIPVARDAIAVIVHPDNPVESLTLQHISDIYSGKITNWKQVGGEDRPIVLLSRE
                     TNSGTHVYFLEEVIRLGDSENKTLFSPDTLLLPSSEGITAEVRQNLNAIGYDGLGYVT
                     PDVKTVAVAAEPGAPYVKPSIESVNAGQYPIARPLFMYTNGEPGGTVKEFIDWILGPD
                     GQAIVARLGFVPLK"
     gene            11631..12545
                     /gene="pstC"
                     /locus_tag="AC162G_04190"
     CDS             11631..12545
                     /gene="pstC"
                     /locus_tag="AC162G_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR5075045.1"
                     /translation="MQQSLSAKRRTSLSEFVAERLIRVSGFIAIGFVVLIFAFLLSEG
                     LPAIAEVPLENLLSTRWYPIEDHFGMMPLLLGSLIVTIGAALISIPIGLLTAVFIAEI
                     APRWLREILKPLVEILAGIPSVVLGFFGVAVMSALVRQTLGLPTGLTAFTGSLILGYM
                     ALPTIVSVAEDALDAVPRSYRDAALALGATKWQTIWMVTVKAAHSGILTAMMLGVGRA
                     IGETMAVMMVTGNAARMPLTLNSLFLPVRTMTATIAAEMGEVAQGSTHFHVLFAIGIA
                     LFVITFLINLFASLAIFRQTRRSARLLS"
     gene            12598..12975
                     /locus_tag="AC162G_04195"
     CDS             12598..12975
                     /locus_tag="AC162G_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR5075046.1"
                     /translation="MPEDKLLHAELTRAIIGAAMEVHNLLGPGFLEAVYEEALAHEFD
                     LRGIAYERQKSYDVPYKTMVAGVYRPDFVVDDKVVVDTKAITKLTEIEEAKILNYLKV
                     TRLRVGLVINFAGRRLEYTRRVL"
     gene            13020..13865
                     /gene="pstA"
                     /locus_tag="AC162G_04200"
     CDS             13020..13865
                     /gene="pstA"
                     /locus_tag="AC162G_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR5075047.1"
                     /translation="MSRFTIQRLGIALLVLVTIAVVVPIVVVVGTIVVQGISAVSAEF
                     LFTAPREGMRAGGIWPAIVGTFWLTAGTAAFSVPLGVAIAIYLSEYARDNNFTRAIRL
                     AIINLAGIPSVVYGLFGLGLFVLALGFGSSILAGSLTLSIMTLPVIISTSEEALRSVP
                     QSFRTVSASLGGTRWQTIRKVVLPNALPGIITGMILGLERAAGETAPILFTAAAFYLP
                     TLPSSIFDKTMALPYHLFVISTQVPGMPPQLQFGTALVLLVFVLSMNLIATIIRSYYR
                     RKRQW"
     gene            13972..14736
                     /locus_tag="AC162G_04205"
     CDS             13972..14736
                     /locus_tag="AC162G_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HIR5075048.1"
                     /translation="MTRTPKIVIENLNFTYEDGTESLIDVSLTIYANEITALFGPAGG
                     GKSTLLRVVNRLNDLVEGSQMSGRVLLDGSDIFAPDASVIELRRRVGIVFAQPMPLPM
                     SIRANVEYGPRLAGVRQASQLAEIVERSLRAAALWDEVNDRLDESGFALSGGQQQRLC
                     LARVLALEPDVILLDEPMSGLDPISTAKIEDSLQQLKQSYTIVIVPHNIQQAARVADR
                     AAFFLQGRLIEVAEGRQLFTNPRDKRTEDYVTGRFG"
     gene            14736..15335
                     /locus_tag="AC162G_04210"
     CDS             14736..15335
                     /locus_tag="AC162G_04210"
                     /inference="COORDINATES: protein motif:HMM:NF022751.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HIR5075049.1"
                     /translation="MGDDRPLMIQGIRIDPSLLKRGAIARCEVVECMAACCTGGVWLE
                     PGEMPRILEWAETIKRHIPPERHDESTWFSAGEPDENMPRGHEMGTTTVNDPMRPGQT
                     CCVFLRSDRKCALQVVSEECHLGWPGLKPFFCAIYPLYFEDGALSMDEDTPLDFEGGG
                     CQRVAPEIRPMYRIYREEAILILGEDGYRELCKRAEESG"
     gene            complement(15353..>16728)
                     /locus_tag="AC162G_04215"
     CDS             complement(15353..>16728)
                     /locus_tag="AC162G_04215"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5075050.1"
                     /translation="TDKRMKADTPHSEISDAVTLTGADELGVEAAPAHGGTFRALRHR
                     NFRLFFFGQMISVIGTWMQVTAIQLLVVSLFDTPEAQAYWLGAVGFVPLIPLIPFALA
                     GGSLADRFPRRTLVIITQSMMMAVAFALYAMTASAAVTIWLVLGLLVVQGAANAVDIP
                     ARQTLVFELVGGRDDLTSAIALNSSVFNLGRALGPALAGILVAAVGVADAFLLNGLSF
                     VAVLVGLLLTRLPPRGMEEKPPRMDKHLREGFGYIKREKTILILLSLIGVTAFLAMPF
                     TQMLPLFATYTLADSAEPLTQALCSPIECSTPEALTLGLLNAFFGFGALVGALGVPSW
                     AERYGRGRVLTLGNVGFPAVLLLFALSRSIWLSLGLLFLVGVGWVMQTTLTNTLLQIM
                     SLDNLRGRVMSVYSMLFQGMWRVGSMGAGLIAGLISAPFAVGLGAALALAYGVLTAIR
                     RPNIRRL"
BASE COUNT         3101 a         5162 c         5152 g         3313 t
ORIGIN      
        1 cgtcggccag cgtgagcaaa cccgactcag ttgcgtagac acgcgctttc aggcgcgcgt
       61 tctcctcgaa cgtctggccg gattcgtcca cctcgaagtc gaccccgacc tcgcgcagac
      121 cgagcaattg gatgccatca tccgacaaaa tggcctggta ctctgcgacc ttgcccggat
      181 tagtcgtcgc cacaaggatt ctagacataa cacttgcata tcttgacagt gtcaggttcg
      241 gctggccgcg ccgtccccgg cgcggtcaac gctgctgagg gctgccctga gcaaaggcga
      301 aggtacggcg gcttgagcct aagtatgaca ttgccgaatt atactcgatt ccaaagaata
      361 tcatgtctac atatttgtga aatttatgtc acatccaaag gtttgcttga cggtaattga
      421 cctgcatgat ataattttat ccgtatttat gggtgttgta cgttgcgttt ggactgagtg
      481 tccacacggc cgtcttcaca ccggtcatgc ggttaaaagt ctctgctctc atacgcgccg
      541 aggttggcgc tcagtcaagc gtgacgatcg accagggcct ctccaatctc ggtcctgatc
      601 tcgtcgtcga ttttgtgcag ggtggcctga cgctcacccg caccgatcgg cacatcctgg
      661 tccaagggag tctgacctcc gcgctcagcg ccgaatgtgt tcgctgcctg gaatcgttcc
      721 gtctcccact tcaggttcag ctcgaggaat tgttcgcgct gattcccggt cctcacgttc
      781 tggatccgct atacgtcgtg gcgccggatg gaacgatcga tctcacatac ccgctgcgag
      841 agaatgtcct gctcgcccag ccgatcaagc cggtgtgccg tcccgactgc aggggtctgt
      901 gcgcgtcctg cggcaagaat ctgaacgaag gcccgtgcga ctgtgaagat gagcttaccg
      961 acccgcgcct ggccgcactg aaggcgctcc tctagacagg gggccgtatg atgacgacgc
     1021 aaggcgacga cgaactggat ttcctggcgg agttgatcga aaaaggcgag gccctgggct
     1081 acctcaccac cggcgagatc ctggaagccg tgcccgagac ggaagacagg gccgagcaac
     1141 tggaaaccgt ccaccagttg ctgcgcgagg cgggcatcga aatttacgaa gactcgatac
     1201 gcatcggcga cgagagcgcg ccggtcgaga tcgaagacct gtccgaggaa gcagacgagg
     1261 agaccgaaga cgacgaagat ttcgacctca gcggcgtggc gagcgacgac acggtcggtc
     1321 tctatttgaa ggagatggcg cgcgtcccgc tcctgaccaa ccaggaagaa gttgcgctgg
     1381 cgaaggctgt gcggcgcgga cgagatgccg ccaaaacgct gcgccgcatg cgtcccagct
     1441 cgcagcggcg ggcgaggctc gagcgctacg tcaacgaagc ccggttggcg cgcgagcacc
     1501 tcatcaaggc caacacgcgg ctcgtcgtca gcatcgccaa gaaatacatc gggcgcggcg
     1561 tgcccttcct cgacctgatc caggaaggca atctgggcct gatgaaagcg gtcgagaaat
     1621 tcgacttcac gcgcggcttt cgcttcagca cctatgccac ctggtggatc cgccagacga
     1681 tcacccgcgc catcgccgac caggggcgca ccatccgcgt gccggtgcat atgagcgacc
     1741 gcatccgtca cttgtacaag acggtgcggc agctcgagca ggcaaacggg cgccagccga
     1801 cggccgaaga gatcgccgag gagatggata tcgagccgcg caaggtgcag tggatgatga
     1861 aagtgtcgtg gcggcccctc tcgctcgaat tgcccgtcgg cgaagacgag gacgccgagc
     1921 taggctcgtt catcgaggac gagacgacgc cctcgcccac ccagacggtt cacgagcgcc
     1981 tgctgcacga gaaggtcgaa gaagtgctca gcacgctcag cccgcgcgag gcgcgcattc
     2041 tgcgcctgcg ctatggcctg gtcaacgggc gctcctatac gctcgaagag gtgggccgca
     2101 agttcggcct gacgcgcgag cgcatccgcc agattcaagg caaggcgctc cgccgcctgc
     2161 gccacccgcg ccgcagccgg gtgctcaggg actaccttca gcagtgacca atctcgaacg
     2221 ccgtgaatcc ggggcgactc gcgcgagtcg ccttttttct tcgccctccc gcaggcccct
     2281 tgctggtttg tctcacccgt gaattatgcc cagccaactc gttcaactcg ccgcctactg
     2341 gaacactccc gccgaccagt tgctgaaatc gttgcaggcg acacctgacg ggcttagcac
     2401 atccgaggcg caacagcgct tgaaagactg ggggccgaac gcgctgaagg tgcgccggcg
     2461 cgtgacccca tggggcttgc tcctcaacca gttcaaaagt ccgctcgttc tcatcttgct
     2521 cttcgccgta gccgtatcgg ctttcgtcaa agaatggact gacgcagcag ttatcctgac
     2581 catcgtggtg ggcagtgcat tgatcacctt ctcgcaggag tattcggcaa ccaacgccgt
     2641 cgaaaaactg cgcgcccgac tgacttacac ggtgatgggg cagcgcgatg ggcgaccaca
     2701 gtctgttccg gccgaggcta tcgtgccggg cgatgtcgtg atgctgtcgg ccggcagtct
     2761 gatccccgcc gacggcgtgg tgttggacgc cagagatttt ttcgtcaacc aggccgtgct
     2821 gacgggcgaa accttcccgg tcgagaagaa gccaggcacg gctcctgaat cagccagcct
     2881 aaccgagcgc accaactgcg tttttatggg caccacagcc cgcagcggcg cggcacgggt
     2941 gctgatcgtc caaaccggtg cagcgactgc atatggccaa atcgccgatc ggttgagcct
     3001 gcgcccgccc gagactgagt ttgagcgcgg cattcgacac tttggcttga tgctgacaca
     3061 ggtaatggta gcgctcatcc tggtcgtgtt cgccatgaac gtgatctcgg ccaagccgcc
     3121 gattgactcg ttgttgtttg cgattgcgct ggccgtcggc atctcgcccg aactgttgcc
     3181 cgccatcatc accatcaatc tatcgaaagg cgcgcgagaa atggcgcagc acggcgtcat
     3241 cgttcggcag ttaaacgcta tcgagaactt cggcagtatg gatgtgcttt gcaccgacaa
     3301 aaccggcaca ttgaccgaag gggtcgtgcg gttggatggc gcgctggatg tgcgtggcaa
     3361 tccatcggat gaagtgttcc gttacgcgta cctgaacgcg cattttcaga ccgggctatc
     3421 caacccgctc gacgaggcca tcacggcaca ccacccactc gacctgagcg atgtcgccaa
     3481 gctgggcgaa gtgccctacg atttcgtccg caagcggtta agcgttgtcg tgggcgaggg
     3541 caatcaaccg cccgttttca ttaccaaagg cgctttggac aatgtgctgg cggtgtgccg
     3601 tcatatccgt ggtggcgagg gggttctccc gcttgacgca gccgagtctg agcggatcgc
     3661 gcagcggttc gcgcaatgga gtgcgcaggg gtttcgtgtg ctcggtgtgg cgattaagtc
     3721 gctgacaccg ccatccgtct attcacgcga cgacgagcgc gacatgattt ttgtcggctt
     3781 tttgttgttc ttcgatccgc ccaagccaga cgcacggctg gcgattgcgg ccctcgcgaa
     3841 actcggcgtg cggctcaaga tcatcacggg cgacaatcgg cgcgtggccg tgcacgtggc
     3901 agaggcggtg ggcatgcctg tggaaggcgt gctgagcggc gcggagctca acgtgctgcg
     3961 tgacgaagcc gtatggcata tcgtcgagca caccgacttg tttgtggacg tcgatccaaa
     4021 ccagaaagag cgcatcattc gcgcgctgaa aaagatgggg cacgtggtcg gctacctggg
     4081 ggatggcatc aacgacgcgc cagccttgca cgatgcggat gtgggcatct cggtggaagg
     4141 cgcagtggac gtagcgcgcg aagcggccga ctttgtattg ttggagcgcg ggctggatgt
     4201 gttgcggcgc ggcatcgaag aggggcgggt cacgttcgcc aattcgctca agtacgtttt
     4261 caccacaacc agcgccaact tcggcaacat gtttagcatg gcgggcgcgt ctctcttcct
     4321 gcccttcctg ccgctccttg ccaagcaaat tctgctcaac aactttctat ccgactttcc
     4381 ggcgattgcc attgccggcg acgatgtcga tcgggagctc gttgagacgc cgcaccgctg
     4441 ggacgtgaag ttcattcgca acttcatgat cgtcttcggc ctggtcagct ctgcgttcga
     4501 ctacctgacg tttggcctgc tgctgtttat tgttcatgcg gcgccggagc aatttcaaac
     4561 cggctggttc gtcgaatcgc tcctgaccga gttgctcgtg atgttcgtcg tgcgcacgcg
     4621 ccggccgttt tacaagagca agccggggcg ctggctggcg atttccacgc tcatcgtcac
     4681 tttgatcacc ctggccattc cgtacctgcc gtttagcgcg ccgttgggct ttgtgccgct
     4741 gccgatggaa gtgatggcat ttctgctatg tattacactg ctgtacttga tcgcgacgga
     4801 agtggcgaag agaattttct acgcgcgggt ggccgttggc gcgtaaagaa ccacgctaaa
     4861 tcggatatac ttctttcatg ctgtcgatcc atcttctggg cacgccgcga attcagcgcg
     4921 acggcgagcc gatttacatc atccgccgca agagccgcgc gctcgtctat tatctcgcca
     4981 cacacgacaa gccgctcacc cgcgaacacc tggtcgcttt cttctggcct gatctcgagc
     5041 gcgcatccgt gcagcagacg cttcgcacca cgcagcacgg tttgcgccgc ctgctcggct
     5101 ccgcgctcgt cgtggacgag gacacactcg cctggcgccg gataccgagg tcgatactcg
     5161 cctcttccag tcacgcctgc tccctcacgc ctgcttgcga tcctcgcaac aggcgcgttt
     5221 cgtttccagg gtgggtcagc ggcaattaga ttatgtcgcg cttttcgaga tgcggctggc
     5281 agcttgtcgg agagagagcg ggccgggctt ccacgccccg caattggcct cgaaacgcgc
     5341 tgaatcggcg gcgtgggagc cgcctctgct gggccctccg acagcctgct agccacgaag
     5401 ccgggcttga cgaagtagct gagcagagat atactctatc tcgaaagcgg aacgtaagtc
     5461 caagcacggc aggagggcag ttgatctgct atccccggca acagccggag caggcctact
     5521 tgggggttcc gttttctatt tcctcttctg cgtccaacgc tgtttgatct cgggccagtc
     5581 ctccaccaga gcgacgctga tcttgccctc gatcatcttt atggtgttgt caccctcctt
     5641 gtgccaagcg tatacactgc cagccacaaa atcagccagt tgaaccagag ggttgcgttg
     5701 gctgtccaca tggctgatcg acagaacacc ctgcgttggc cagtggcggt agagaaacgt
     5761 gtttagaaca gccacctgtg ccggtgaagt aaagcgccgg tcaatcgcaa gcgcaacgtt
     5821 gggatgcgcc agccagcacg cttgaagcac cgcgcacacc aacactgcat agttttccgg
     5881 tgtatcttcg attttccgcc cgcccttacg caccgtcaac gcaaatatct cgatgtcggc
     5941 tcgtgcgagt cggctcaaca catctgctcg aaggcggcgg ctagtgttgt gccacttgag
     6001 ttcggaaaca gccctgcgtg gtcgtttgag ccgcttcccg gaacgcacgg cggcccgttg
     6061 gatcaggtat cggatagtgt ccggttggta cgtcagcact ccgaccagtg tgaatagcga
     6121 cgtgtcggct gatagggagc ctgattcgtc caggccgaca taggcaagcg tcacagatga
     6181 cattcatcta cattctgtgc aaccaattca gccgcgattc ctttcatttt cggtcttgcc
     6241 cgccatcgcg tcttggccag tataggcggg atcgcttcga tcctttttgg aatcaacttc
     6301 attcgcttgc gtcactatgg cagtggcccg cgccagcatc gaccggatct cgccgccgat
     6361 ggccgggttc agattgaaag tgagcggctc gaaagtgcag atgtcggcag cggtgatttg
     6421 agtcgggtcg tgggtgatga agttcgtgaa gagctccgcg tcgtcgtcca gatacagcaa
     6481 ccgccggctc tcttcgccgt gcagtttgtc ggcgacggct gtgtacaggt tggccagagt
     6541 atcggtatct tccgatccaa ttccttcgcc caactgcccg gcgttttcac gctggctgat
     6601 gatcagcccc atgcgatatg gccactgctc ggtgaggatg acccatttga tcagttgacc
     6661 cgctcgttcg gcagtcaggc ccgattggct gcgcttgtcg agcatgcgca cgagcctgta
     6721 gatgttgaca atgcgttttg cccggcgcgg gttgcgcgag atatactttg caaagtcgtc
     6781 gaaggccatc tgctcgtcgt cggtgaagct cacttcgacg gcaggcggcg gcgtctcggc
     6841 ctgcggagta ctggcctgcg gtgctgtggg tcggaccagc gcgcgggctg actcttcgcc
     6901 gggacgcggt agcagcgcgc ggatgtagtt cttgatcccg gtttcgtcgg tcgcggggat
     6961 cgtgaagggg atctgcacga tcttgtcgag gtactcgtag ccactgatgc cggcctcgac
     7021 tagtgtcttg ccatatcgat cctcgatcgc gcgaaccagc acgcgcgcgt caatgccgag
     7081 gaagacatag aacggcgagt tgtcttccga cagcagcagc atgatcgctt ccagcacttc
     7141 gacggcttta gccggcgggc agcgatccag gccatcgatg aagattacga ctcgaatcga
     7201 cttgccctta ctttggtgtg ccagcaactc ggtcacgaag tcgatctcgg ctttgacgtc
     7261 ggccatgaag ccgattttgg ttcggaaatc aggctgggcc gcctcctgtc tgagttgctc
     7321 ggcgcgctca acgattgctt tctttacaat atcgtaaatg ctcttgatcg tgcggacaaa
     7381 gccgacgatc agagcgagga aggacgatac gccaagcaat gcgagaccgg gcaaatctgc
     7441 ccagatcttc gcaatttggt ctagactgag aagcaccgtg accgcgatga tgggaattgc
     7501 atacaggatc gcgtaaaacg acagacgcct cacttgcttg cggcgcttcc tatcgagtcg
     7561 aaaggaacgt gtggcgttgg aacctagccg ttgctcgatg cgctcataaa tgtggctgac
     7621 tagcccggcc cacaggttgt cgctgccact gtagacccag gcgttgaatt cgacgaagga
     7681 gaactccggt gcgtcagcag gcttgtcgtt ttctagcgct tcgcggatca tccgtatcag
     7741 gaacgatttg cccatgcccc acgcaccgta aatgccgatc gtcagcgcgg ctgggtggcg
     7801 ggattgctca gcatggccgc gaaggcccgg gcgtagtcgg tgaagccgag tcgatcgtcg
     7861 ccgcgcgggc gatcgttcag cgcgaaggtt gagcctattt ggatgggcgc gccgggggcc
     7921 tcgcccggca acacttcgtc ttccttgagg aagccttcgg cgacgagttt ttccttcaac
     7981 tccagatacg acttggtgcc gaagttgcgt atcgtgagca tcgcgtcctc gccgctggcg
     8041 agcatttcca ggacctcgcc gacgttggtg atgcccgtgc gcttgagcga attgaacacg
     8101 cgctccgaca ggtcgagctg ttcgatcggc gtctggtaga tttcctgcgg gatccaggag
     8161 gagacggcgt ctttctgttc attgggagtc tttctgggaa tcgcggtcgg ttgatcgctc
     8221 atggtggtgc ccttctcgcc ggggcctagc cgaatcgtcc cgtgatgtaa tcctcggtct
     8281 gcttctgttc aggttggacg aacagcgcct cggtggcccg cgctcgacca actcgcctaa
     8341 ccaaacgaag ccggtaaagt cgctcgcgcg tgcggatcac ttcgcttcca gttccttcac
     8401 caacgcctcc gctccgacac gctgcacgag gaagtaggca atggcggctt tctggctggc
     8461 ctcgttcaca tcgtgtgccg gttcggcagc ctgtctcacc cagttgtgca cttcatccga
     8521 ggccggtagg ctcaggccga agtcgatcaa ctctttgagc gtgtattttt cgatcagccg
     8581 ccgggcgaca ctctcctgcg tgacctcgcc cggcgctttg gccacaggct ggctcagcag
     8641 caagtccacg taaccctggc cgccgttctc caggatcatc agcgccatgc tcgtccgtgc
     8701 gaaattggcg ggcgcttttg ggtcgaggag cctgtccaga tcggccagcc gcgcctgctc
     8761 ctcttccacg gtcagcgtcg cccgcgccag gatcgtgtac gtggcgatgt cgtgcagctc
     8821 ggccaggctg gggtaacgct cgatcatgcg ggacactgcc gtccggcgac ccttcatcaa
     8881 ctccagatcg atctgcgtct tgcacaggtt ctggaactgc tcgtacagcc agcccaggtt
     8941 gaggctgacg tcgccgttcc ccgatccgcc gatttgcttg gcgatggtga acttggtgcg
     9001 gatgagcgcc tggaaaccga tgcccacggc aatgaccgtc agcaactgat cggccgccgg
     9061 cgtggacgcg cgcacgatcg cgtatgcgag gcccgccgcc accgcgttca ccgcgatgag
     9121 aaaccgcgcc caggtagtgc gcaaagcctc gcgcggataa ttggaaaacg tcgacgtgat
     9181 ctcggccagg ccgacgatcg cgccgagcaa cacgacgatg gcatacggcc cggcctgggc
     9241 caaggcggcg cgaagctgcc cctggaactg cggcgcccac ggctgaacgg gcatctgctc
     9301 gaacgggagc gccagaaaaa cgacgagggc caggatcacc aggacggcta acaccgccca
     9361 aacacccggc ccccgttccg ctttgtccgg aggaggcgac gcgccgctga tacccggagt
     9421 tggcgccatc gctcacctct tcatcttcat ctgcttcatc gatctggcgc tgcctatgtc
     9481 cagcatgtcg cgggcgcgcg ccgtgagcgc gaccagcttg ccgtcggcgc tgtccgtgtc
     9541 gtctcgcatc acgaccagat cggcatcggc tagcgtcttg agcagtttca gcgcgtcgcc
     9601 gggcaagatc cacgtttcgg ccgacacctg gctggggctc atcgcaccgc ccttcgccag
     9661 agcgagcagc actttccgct cttcgtcgtt caaatcgctc ctcgtgatgt ccatgttgcc
     9721 gcctccttta ggaatcctcc tgccccatcg attatatcac gaaccttgcg agccttgcga
     9781 aggcgcgaag tgtgaacctt cgcaaggtta cccaaacctg cctgtgatat attcctcggt
     9841 ctgccggtgc ttcgggcgga ggaagacctc ttgcgtcggc ccgtgctcga ccagctcgcc
     9901 cagccagaag aagccggtga agtcgctcgc gcgcgcggcc tgctgcatgt tgtgcgtgac
     9961 gatcacgatc gtataattct ttttcagctc ctgcatcaag tcttcgatct tgagcgtggc
    10021 gatcggatcg agcgccgagc agggctcgtc catcaggatc acctcgggct gcacggccag
    10081 cacgcgggcg atgcacaggc gctgctgctc gccgagcgac aactcgagcg ccgattgctt
    10141 gagattatcc ttcacctcgt cccacagcgc cgcgccgcgc agcgactgct cgatcaggtc
    10201 gtccagactg gccggcttca tgcgcagcgc gcgcgggccg aaggtgacgt tgtcgtagat
    10261 cgactgtggg aacgggttag gcctctgaaa gaccatgccc acgcgccgcc gcaggtcgac
    10321 gacgtcctgc ttcgggctga gcacgtcgac gccgtccagg agcacccgcc cctcggcgcg
    10381 cgcgccgggg atcgtgtcgt tcatgcgatt gaggcagcgc aggaaggtcg atttgccgca
    10441 gccgctcggg ccgatcagcg cggtgatgtg ccggtcgggg atgccgatcg tgacctctga
    10501 cagcgcctgg ttgccgtcgt aatagaaatt cagatgctcg acttcgatct tgttcacggt
    10561 gtcgattgta acgtgaagcg cggcaagtca caaatctggt gattatccac aagtccgttc
    10621 gtccgcagtt gcactgcgga cgccgggcga cagtgcaact gtcgccccaa catgtgtgca
    10681 atcaccagtc acaaatgcat tgttgaagcg cgccgggcat gttataatcg cgccgatgcg
    10741 cccctcgacc ctttgcctga tgctgtgcct gacgctgctc gtcggatgcg ccgggcgggc
    10801 gactgcgcag gatgacgcgg ccggcggcgc cgcgatagaa aacatcggct cggacaccat
    10861 ggtcaatctg gcgctggcct gggccgaggc gtatcagggc atttaccccg acgtgcgcat
    10921 ttcggtcacc ggcgggggat cgggcaccgg gattgcttca cttatcaacg gcacggcaga
    10981 catcgccaac gccagccggg cgatgaaatc tgaggaacga gccgaggccg aatcgaacgg
    11041 tatccagccg gttgagatcc cggtggcgcg cgatgccatc gcggtgatcg tccacccgga
    11101 taaccccgtc gaatcgctca cgctgcagca catctccgac atctacagcg gcaagatcac
    11161 caactggaag caagtcggcg gcgaagacag gcccatcgtc ttgctgtccc gcgagacgaa
    11221 ttccgggacg cacgtctact tcctggaaga agtcatccgg ctcggcgata gtgagaataa
    11281 gaccctcttc tcgcccgaca cgctgctgct gccgtcgagc gaaggcatca ccgccgaagt
    11341 gcgccagaac ctcaacgcca tcggctacga cgggctgggc tacgtgacgc cagacgtgaa
    11401 aacagtggcc gtggccgctg agccgggagc gccgtacgtc aagccgagca tcgagtcggt
    11461 caacgccggc cagtacccga tcgcgcggcc gctgttcatg tacacgaacg gcgagccggg
    11521 cgggacggtt aaagagttca tcgactggat tctagggccg gatggccagg cgatcgtggc
    11581 acgactcggc ttcgtgccgc tgaagtgatc gagtcaccgg tgactgatct atgcaacaat
    11641 cgctttccgc caaacgcagg acgtcgctct cggagttcgt cgccgagcgc ctgatccggg
    11701 tgagcggctt catagcgatc ggctttgtcg tcctgatttt cgccttcctg ctgagcgaag
    11761 ggcttcccgc gatcgccgaa gtcccgctcg agaatctcct ctcgacgcgc tggtatccga
    11821 tcgaggacca tttcggcatg atgcccctgc tgctgggatc gctcatcgtc acgatcgggg
    11881 cggcgctgat ctccatcccg attggattgc ttacggcggt cttcatcgcc gagatcgcgc
    11941 cgcgctggct gcgcgagatc ctcaagccgc tggtcgagat cttggccggc attccgtcgg
    12001 tggtcttggg tttcttcggc gtcgccgtca tgtcagccct cgtgcgccag acactgggcc
    12061 tgccgaccgg gctgacggcc ttcaccggct cgctgatcct gggctacatg gccttgccga
    12121 cgatcgtcag cgtggccgag gacgcgctcg acgccgtgcc gagaagctat cgcgacgcgg
    12181 cgctggcgct gggcgcgacg aagtggcaga cgatctggat ggtgacggtg aaggctgcgc
    12241 acagcggcat cttgacggcg atgatgctgg gggtgggccg cgccatcggc gagacgatgg
    12301 cggtgatgat ggtcaccggc aatgcggcgc gcatgccgct gacgctgaac tcgctcttcc
    12361 tgccggtgcg cacgatgacc gccacgatcg cggcggagat gggcgaagta gcgcagggca
    12421 gcacgcactt ccacgtcctg ttcgctatcg gcatcgcgct gtttgtgatc acctttctca
    12481 tcaacctgtt cgcgtcgctg gcgatcttcc ggcagactcg ccgctccgca cgcctgttat
    12541 cttaaccacg gacgacacag attttcacgg atgttgtttg gcgggatggg ggcctgaatg
    12601 ccggaagaca agctgcttca tgccgaattg acgcgtgcca tcattggcgc ggcgatggag
    12661 gtgcacaatc tcctcggccc tggttttcta gaggcggttt acgaagaggc gctggcgcac
    12721 gaatttgact tgcgcgggat cgcttacgag cggcagaagt cgtatgacgt gccatacaag
    12781 acgatggtag cgggcgtgta tcgccccgat ttcgtcgtcg atgacaaagt cgtcgtggat
    12841 accaaagcca taacaaagtt gaccgagatc gaagaggcta agatcctcaa ctacttgaaa
    12901 gtgacgcgac ttcgcgtcgg gttagtgatc aacttcgccg gacggcgtct agaatacacc
    12961 cggcgtgtct tatgagttga tacatctgtg taatccgtgt catccgtggt ttattttgta
    13021 tgtctcgatt tacgattcaa cgcttaggca ttgcgctgct ggtgctggtg acgatcgccg
    13081 tcgtcgtgcc gatcgtcgtc gtggtcggga cgatcgtcgt gcagggcata tcggccgtca
    13141 gcgccgagtt tctcttcacc gcgccgcgcg agggaatgcg cgcaggcggc atctggccgg
    13201 cgattgtcgg cacgttctgg ctgaccgccg gcacggcggc cttctcggtg ccgctcggcg
    13261 tggcgattgc gatctacctc agcgaatacg cgcgcgacaa taacttcaca cgcgccatcc
    13321 gcctggcgat tatcaatctc gccggaatcc cgtcggtcgt gtacggcctg ttcgggctgg
    13381 ggttgttcgt gctggcgctc ggcttcggct cgtcgatcct ggctggatcg ctgaccctct
    13441 cgatcatgac gctgccggtg atcatcagta cctcggagga ggcgctgcgc tcggtgccgc
    13501 aaagcttccg caccgtgagc gccagcctgg gcggcacgcg ctggcagacg attcgcaaag
    13561 tggtgctgcc gaacgcgctg cccggcatca tcaccggcat gatcctgggg ctggagcgcg
    13621 cggccggcga aaccgcgccg atcctgttca cggccgccgc gttctatttg ccgaccctgc
    13681 ccagcagcat cttcgacaag acgatggctt tgccttatca tctgttcgtg atcagcacgc
    13741 aagtgcctgg catgccgccg caattgcagt tcggcacggc cttggtcctg ctcgtattcg
    13801 ttctatctat gaatctgatc gcgacgatca tccgcagcta ctatcgccgc aagcgccagt
    13861 ggtgaacgaa accacagatg acacggatta cacagatgtt attatccgtg tcatccgtgc
    13921 tatccgtggt taggcatccg tgctatccgt ggttagacat ccgtggttga aatgacccgc
    13981 acgccgaaga ttgtcatcga gaacctgaac ttcacgtacg aggacggcac ggagagcctg
    14041 atcgacgtga gcctgacgat ctacgccaac gagatcacgg cgctgttcgg gccggcgggc
    14101 ggtggcaagt cgacgctgct gcgcgtcgtc aaccgcctga acgatctggt cgagggcagc
    14161 caaatgtccg gccgggtgct gctggatgga agtgacatct ttgcgcccga cgcgagcgtg
    14221 atcgagctgc ggcggcgagt gggcatcgtc ttcgcacagc ccatgccgct gccgatgtcg
    14281 atccgcgcca acgtcgagta cgggccgcgg ctggcgggcg tgcgccaggc gagccagctc
    14341 gccgaaatcg tcgagcgcag cctgcgtgcg gcggcgctgt gggacgaagt gaatgaccgc
    14401 ctggacgagt cgggcttcgc cctatcgggc ggccagcagc agcggttgtg cctggcccgc
    14461 gtcctggcgc ttgagccgga cgtgatcctg ctcgacgagc cgatgtctgg cctcgatccg
    14521 atctcgacgg ccaagatcga ggactcgctc cagcaattga agcagtcgta cacgatcgtc
    14581 atcgtgccgc acaacatcca gcaggcggcg cgcgtcgccg atcgcgctgc cttctttctg
    14641 caggggcggc tgatcgaagt cgcagaggga cggcagttgt tcacgaatcc gcgtgacaag
    14701 cggactgagg attacgtgac cgggcggttc gggtaatggg cgacgaccgg cccctgatga
    14761 tccagggcat ccgtatcgac cccagcctgc tcaagcgcgg cgcgatcgcc cgctgcgagg
    14821 tggtcgagtg catggctgcg tgctgcaccg gcggcgtatg gctggagccg ggtgagatgc
    14881 cgcgcatcct ggagtgggcc gagacgatca agcggcacat cccgcccgag cgtcacgacg
    14941 aatcgacgtg gttctccgcc ggcgagccgg acgagaacat gccgcgcggg cacgagatgg
    15001 ggacgaccac cgtcaacgat cccatgcgcc ccgggcagac ctgctgcgtg ttcctgcgat
    15061 ccgaccgcaa gtgcgccctg caggtcgtgt cggaggagtg ccacctggga tggcctgggc
    15121 tgaagccatt cttctgcgcc atctacccac tgtacttcga agacggcgcg ctcagcatgg
    15181 acgaggacac gcccctcgac ttcgagggcg gcggctgcca gcgtgtcgcg cctgaaatca
    15241 ggccgatgta tcgcatatac cgcgaggaag cgattctgat tctcggcgaa gacggatatc
    15301 gggagttgtg caaacgggca gaggagagtg gataatcgcc cgtcatccat cactacaatc
    15361 gccgaatgtt gggtcgccgg atggctgtga gcacgccgta tgccagcgcc agcgccgcgc
    15421 ccagcccaac ggcgaacggc gcactgatca acccggcgat cagtcctgcg cccatactcc
    15481 ccacacgcca catcccctga aacagcatcg agtacacact catcacgcgt ccgcgcaggt
    15541 tgtcgagcga catgatctgc agcaaggtgt tcgtcagcgt ggtctgcatc acccagccga
    15601 cgccgaccag gaacagcagc cccaacgaca gccagatcga gcgcgatagc gcgaacagga
    15661 gcagcacggc cgggaacccg acgttgccga gcgtcaatac acgtccgcga ccgtatcgct
    15721 cggcccacga ggggacgccg agcgccccga cgagcgctcc aaatccgaag aaggcgttga
    15781 gcaagcccag cgtcagcgcc tcgggcgtgg agcattcgat cgggctgcac agcgcctgcg
    15841 tcaaaggctc cgcgctgtcg gccagcgtat acgtcgcgaa cagcggcagc atctgtgtga
    15901 agggcatcgc cagaaaagcc gtcacgccga tcaggctgag caagatcaag atcgtcttct
    15961 cgcgcttgat gtagccgaat ccctcccgca ggtgcttatc catgcggggg ggcttttctt
    16021 ccattccgcg cggtggaagt cgggtcagca gcagtccgac cagcacggcg acgaaggaga
    16081 ggccgttcaa caggaacgca tccgccacgc ccactgccgc gacgagaatc cccgccagcg
    16141 ccgggccgag cgcgcgcccc aggttgaaga ccgacgaatt gagcgcgata gcgctcgtca
    16201 aatcgtctct gccgccgacc agctcgaaga ccaacgtctg ccgcgccgga atgtctacgg
    16261 cattggcagc cccctgaacg accagcaagc cgaggacgag ccagatcgtc accgctgccg
    16321 acgccgtcat cgcgtacagc gcaaacgcca cggccatcat catcgactgg gtgataatga
    16381 ccagggtgcg gcgtgggaac cgatcggcca gcgagccgcc ggccagcgcg aacgggatga
    16441 gcgggatcag cggcacgaac ccaaccgcgc ccagccagta agcctgcgct tcgggcgtgt
    16501 cgaacaggct gacgacgagc aactggatcg ccgtcacctg catccacgtg ccgatgaccg
    16561 agatcatctg gccgaagaaa aacagccgga agttgcggtg gcgcagcgcg cggaaggtac
    16621 ccccgtgcgc cggcgcggcc tccacgccaa gctcgtccgc accggtcagg gtgacggcat
    16681 ctgagatttc agagtgcgga gtgtcagcct tcatccgttt atccgttg
//
LOCUS       DBNHXB010000109         9043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_21093, whole genome shotgun sequence.
ACCESSION   DBNHXB010000109 DBNHXB010000000
VERSION     DBNHXB010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9043
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_21093"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /gene="cgtA"
                     /locus_tag="AC162G_04220"
     CDS             complement(<1..588)
                     /gene="cgtA"
                     /locus_tag="AC162G_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HIR5075051.1"
                     /translation="MSFDVARIHVKGGDGGNGAVSFRREKYVPFGGPDGGDGGRGGSV
                     YLVVNPQMNTLNYFQRHRRFKAAPGRPGRGKDQTGADGHDVTVEVPSGTVVRDAGTGA
                     VYGDLVKPGQRLLVARGGRPGKGNARFATPTNQAPRMATNGEPGEERTLLLELKLLAD
                     VGLVGKPNAGKSTFLAATTAARPKIADYPFTTLQPN"
     gene            complement(709..1119)
                     /locus_tag="AC162G_04225"
     CDS             complement(709..1119)
                     /locus_tag="AC162G_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075052.1"
                     /translation="MFDPTTHWFYSGKMPSPGAIAENMRHEFWEQHVYGVWFTCTRTE
                     NPHLFRGLWQNKQFEVEVEPRRYVIVRSKEELPWIIEGFKRGLRSVPTFKYQEADGRF
                     AVEWRIDGREARWQSMQGKPAFKNLKRLDMRPGA"
     gene            1447..1971
                     /gene="coaD"
                     /locus_tag="AC162G_04230"
     CDS             1447..1971
                     /gene="coaD"
                     /locus_tag="AC162G_04230"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR5075053.1"
                     /translation="MTKAVYPASFDPIHYGHLDIIRRAAAIFDEVIVGVYDRPMKNLL
                     FDIHERLALAREAVAGIPRTRVTSYSGLTVDFAREAGAQVIVRGLRVVSDFELEWRMA
                     LTNKQLAPDLEMICLMTSREYAFLSSSTVREVALLGGDVSGMVPPPAARALARKAAAL
                     AGKSHGIAPASLAD"
     gene            2004..2501
                     /locus_tag="AC162G_04235"
     CDS             2004..2501
                     /locus_tag="AC162G_04235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit B family protein"
                     /protein_id="HIR5075054.1"
                     /translation="MDILYLIDRLEALLNEGRHLPLTKGVVVDEQRAWDLIDQMRIAI
                     PDEIKKAKRVNQERDRIIAQAHEEAGRVVDLAREEASGLVSDHELTRSAESRAETIVE
                     RARRDAEVIKADADDYVMQVLSELDINLTKTLSVVRNGLAKLQAEHQAAAMRSASEEA
                     PSSNS"
     gene            2563..2775
                     /gene="rpmF"
                     /locus_tag="AC162G_04240"
     CDS             2563..2775
                     /gene="rpmF"
                     /locus_tag="AC162G_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013908.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR5075055.1"
                     /translation="MTPLPKRKLSKGARNRRRSHLALKPGSLSVCPSCKSAMRPHQVC
                     PECGKYRGVEVIDVEARAAKKSKSQR"
     gene            2851..3792
                     /gene="fabD"
                     /locus_tag="AC162G_04245"
     CDS             2851..3792
                     /gene="fabD"
                     /locus_tag="AC162G_04245"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR5075056.1"
                     /translation="MTSAATAFLFPGQGSHHVGMGRALAETYPEARDVFAQADEVLGF
                     PVSALCFEGPEAELTDTINQQPAILIDSVAALRVVEARAPETRPAFLAGHSLGEFTAL
                     VAAGTLAFADAVQLVRERGRLMKQAGALAPGGMAAILNLDRETLADACREAEAGTGKR
                     VQIANDNSPGQIVISGDKAALDKAMELAKAKGARRVIPLAVSIAAHSHLMQPIVRSFR
                     GELDSVAFAPMKIPVVGNVYARPIDFVDIRDELEAQLTAPVRWTETIQYLAARGVTRF
                     VEVGPKDVLAGLVRRIDPSVTAMSVGDPAGIEWLVAS"
     gene            3789..4694
                     /locus_tag="AC162G_04250"
     CDS             3789..4694
                     /locus_tag="AC162G_04250"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007694090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5075057.1"
                     /translation="MISVPRIGFIGLGLMGKPMAGHLVRAGYQVTVHNRSRAAVDELV
                     AQGGRAADNPKGVAAESDVVITMLPDSPDVEAVAFGEQGILAAARPGMIHIDMSTIAP
                     AAARRIAEAEHHAGVENLDAPVSGGDVGAKNATLTIMAGGKPDVFQKCLPIFERLGKK
                     ITLIGDHGAGQIAKACNQIMVAAQMVAMGELLVFAQKAGADPREVVEAIKAGAAQCWT
                     LDTKPQRLFAGNRAPGFKSSMMLKDLNIIVDSAKAYSIPLPTTAVNAQLFAALVAAGQ
                     GDLDNSAVIGVIEELARVKLLEIGD"
     gene            4745..5491
                     /gene="fabG"
                     /locus_tag="AC162G_04255"
     CDS             4745..5491
                     /gene="fabG"
                     /locus_tag="AC162G_04255"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR5075058.1"
                     /translation="MQLKDKVAVVTGASRGIGRAIAIELARRGAKVVVNYNASAGAAG
                     EVVSVIKEAGGEAIAVKADVSRLDEAQALIKAATETFGRLDILVNNAGTTRDMLLAMM
                     KEEDWDLVLATNLKSAFNCSKAALRPMMRQKYGRIVNITSVAGIAGNPGQTNYAASKA
                     GLIGFTKSLAKEIGPRHITVNAVAPGFVETELTKDLPADLKEAALKATPLGRWGTAEE
                     MAYAVAFLASDQAAFITGQTLSVDGGLAMQ"
     gene            5513..5890
                     /locus_tag="AC162G_04260"
     CDS             5513..5890
                     /locus_tag="AC162G_04260"
                     /inference="COORDINATES: protein motif:HMM:NF015721.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR5075059.1"
                     /translation="MAAENVSESATAPDAGAGLAPAHRGRVTVAQRVLDAIAQLTTLN
                     VPGVVRLATRGGLRRGSDGVRVDVVDDKVFVDVYVIVTHDANMRQIGKEIQVEIARAM
                     NQIVGMDAAEVNVHIQDVEPPAA"
     gene            5907..6344
                     /gene="nusB"
                     /locus_tag="AC162G_04265"
     CDS             5907..6344
                     /gene="nusB"
                     /locus_tag="AC162G_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR5075060.1"
                     /translation="MKGRRRARSVALQALYELDSTHHDPGTVLAHRFSDVRLKPEGEA
                     FARALVAGVLRERDRLDALIQRHAPEWPIDQLAVVDRNVLRIAIFEFDIAKITPMRVA
                     INEAIELAKEFGADSAPRFVNGVLGAIASAPDASTKHVEAAKK"
     gene            6346..6822
                     /locus_tag="AC162G_04270"
     CDS             6346..6822
                     /locus_tag="AC162G_04270"
                     /inference="COORDINATES: protein motif:HMM:NF014470.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033281 - TAT protein transport complex
                     [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA/TatB"
                     /protein_id="HIR5075061.1"
                     /translation="MGEILGIGIEELIFVGILIALLFGPESIPKMARTAGRALNRLFR
                     SPLYRESQQIRKQIQDLPAALARLAELEEMQKNLNNEISDLRRSIDPGLSQTAPSTTI
                     SPLQPAATTPGPDAQVEPPTSDASPREPDAPEADHSIEPPHAPPRPAADEPSHATR"
     gene            6809..7729
                     /gene="tatC"
                     /locus_tag="AC162G_04275"
     CDS             6809..7729
                     /gene="tatC"
                     /locus_tag="AC162G_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR5075062.1"
                     /translation="MPLVDPALLVIALFAFSFLFSLLAILRNLRVMRAEAAEEDGDLA
                     GTLETVTQDWRVILPHLVELRNRIFYSVIMLAVTTAISFTFTSQLLDALMAPLPSDAI
                     VVAATITDQLGMFMRIALTSGLALAMPFIATQFWIFISQGLKAGERRYVYWFVPGATI
                     LFITGAAFAYFVMLPAAIPFLLSIFEDVPNYLRLSDYVADVTRLLMWIGLSFEIPLVI
                     AALARVGLITARQLVQGWRFAVVAIAVLAAIVTPTADPVNMALVMAPLFVLYLLSILL
                     AALVRREKRSPRPKPLSKRRLSLLKRRSRT"
     gene            7824..>9043
                     /locus_tag="AC162G_04280"
     CDS             7824..>9043
                     /locus_tag="AC162G_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HIR5075063.1"
                     /translation="MLRTRVLIMGAAGRDFHNFNVFFRDNERYEVVAFTATQIPNIEG
                     RVYPDVLAGKMYPHGIPIYPESELRRLIRDLDVDQVVFAYSDISHEHVMHKASEVLAA
                     GADFRLMGLKATQIKSSKPVVSIGAVRTGSGKSQTTRRVTQVLKELGYTVAAIRHPMP
                     YGNLVKQTAQRFADYDDLDEYDCTIEEREEYEPHIDRGVTVFAGVDYGRVLEMAEGEA
                     DILVWDGGNNDLPFYKSDLHIVVVDPHRPGHETAYHPGEANLRAADVVVINKIDTADF
                     KNVAAVRENVYAVNPTAQIVDGASPIFVDDPGAIRGKRVLVIEDGPTLTHGEMAYGAG
                     VIAAQRFGAKEIVDPRPYALGSILDTYRKYPTTGSVLPAMGYGERQTKELEETINGTP
                     ADMVIIATPIDLRR"
BASE COUNT         1725 a         2928 c         2854 g         1536 t
ORIGIN      
        1 attcggctgc agcgtcgtga acggatagtc ggcgatcttg ggacgagcgg ccgtcgtggc
       61 cgccagaaag gtggactttc cggcgttagg tttgccgacc aggcccacgt cggccaagag
      121 cttgagctcg agcagcagag ttcgttcttc gccaggctcg ccgttcgtcg ccatgcgcgg
      181 tgcctggttc gtcggcgtcg caaaacgcgc attgcccttg ccggggcgcc cgccgcgcgc
      241 gacgagcagc cgctggccgg gtttgacgag atcgccgtac acggcgcccg tgcccgcgtc
      301 gcgcacgacg gtgcccgacg gcacctcgac cgtcacatca tgaccatccg cgccggtctg
      361 atccttgccg cggccaggcc tgccgggagc ggccttgaaa cgccggtgcc gctggaagta
      421 gttcagcgtg ttcatctgcg gattgacgac aaggtagacg ctgccgcccc ggccgccatc
      481 gccgccatcc ggcccgccga acggtacata tttctcgcgg cggaaagaca cagcgccgtt
      541 tccgccatcg ccgcccttaa cgtggattcg tgcaacgtcg aatgacatcg gcgcaaggat
      601 acctatatgc ggagaatgtg tcaaaacgaa aagagggtgg cgctctgcca ccctctcacg
      661 tcaacctgga gtagaatcaa ccgccgtggc ttcccccaaa acatcgggtc atgcgccggg
      721 gcgcatgtcc agccgcttca ggttcttgaa cgccggtttg ccctgcatgg attgccaacg
      781 cgcctcgcgg ccatcaatcc gccattctac cgcgaaacgg ccgtcagcct cctggtactt
      841 gaaggtcggc acgctgcgca gcccacgttt gaagccttca ataatccacg gcagctcttc
      901 cttagagcgg acaatgacgt acctgcgcgg ttccacctct acttcgaact gcttattctg
      961 ccacaaaccc cggaacaaat gcggattctc tgtgcgcgtg caggtgaacc agacgccgta
     1021 cacatgctgc tcccagaatt cgtgacgcat gttctcggca atcgcgcctg ggctgggcat
     1081 cttgccagag tagaaccagt gggttgtcgg atcgaacatc gagcctccat caaatagcca
     1141 atatgaacac tgctgtgcac gagcaaagtc acgcagccgc atgtgttatg ataacacaag
     1201 atcagggatt cggacagcat gcgacaattg acaccgactt gaaggcgtgt cgccgcgaag
     1261 tttccaaatt cgcctgatct gaatggctgt tctataatgg gtatagcgtt accagcaaga
     1321 ctgggttatc tcacgatctc aaacgctcta caatggcggc ttcagtacag tcgagctcac
     1381 ccgcaatacg actttggcct ttagcgtgat atacttacac aacttgaatc gtataggaaa
     1441 ctccgaatga ccaaagccgt gtaccccgcc tcgttcgacc cgattcacta cggccacctc
     1501 gacatcatcc gccgcgccgc cgcgattttc gacgaagtga tcgtcggcgt gtacgatcgc
     1561 ccgatgaaga acttgctgtt cgacattcac gagcggctgg cgctggcgcg ggaggcggtc
     1621 gccggcatcc cgcgcacacg cgtcacgtcc tacagcggcc tgacggtcga cttcgcccgt
     1681 gaggccggcg cccaggtcat cgtacgggga ttgcgggtgg tgtcggactt cgagctcgaa
     1741 tggcgcatgg cgctgacgaa taagcagctc gctccggatc tcgaaatgat ttgcttgatg
     1801 accagccgcg agtacgcatt cctgagctcg agcacggtgc gcgaagtcgc gctgctgggc
     1861 ggcgacgtct ccggcatggt gccgccgccg gcggcgcgcg cactcgcgcg aaaggcggcc
     1921 gcgctggccg gcaagagtca cggcatcgcg cccgcctcgc tcgccgattg acgcgcccac
     1981 atctgcctcg ccgggaggac atcgtggaca tcctttacct gatcgaccga ctcgaagccc
     2041 tgctgaacga aggccgccac ctgcccctga cgaaaggggt tgtggtcgac gagcagcgcg
     2101 cctgggacct catcgaccag atgagaatcg ccatcccgga cgaaatcaag aaagccaagc
     2161 gggtgaacca ggagcgcgat cggatcatcg cgcaggccca cgaggaagcc gggcgcgtcg
     2221 tcgatctggc gcgcgaggag gcgtcgggac tcgtctccga ccacgaattg acgcgcagcg
     2281 ccgaatcccg cgctgagacg atcgtcgagc gcgcccgccg cgatgcggag gtcatcaagg
     2341 ccgacgccga cgactacgtc atgcaggttc tcagcgagct ggatattaat ctgaccaaga
     2401 cgctgagcgt ggtgcgcaac gggctggcca agctgcaggc cgagcaccag gccgcggcca
     2461 tgcgctcggc gtccgaagaa gcgccatcat cgaattcttg acacgccttt ccgctctgtt
     2521 ataatacgtt ggcactcagt tccagaagga gtttagattc tcgtgacacc cttaccgaag
     2581 cgaaagctat ccaagggggc gcgcaatcgc cgtcgcagtc acctggcgct caagcccgga
     2641 tcgctgagcg tgtgtccctc ttgcaagtcg gcgatgcgac ctcaccaggt ctgcccggaa
     2701 tgcggcaagt accgtggtgt tgaagtgatc gacgtggaag cgcgggcagc caagaagagc
     2761 aagtcgcagc gttagtcctg ccggccggcg gcgcgcatca cgggctgctg cctcgaacat
     2821 ttccatatcc acatcacagg gagttgacgg gtgacgtccg ccgccaccgc ctttctcttt
     2881 cccggccagg ggtcccacca cgtggggatg ggccgggcgc ttgccgagac gtatcccgaa
     2941 gcccgcgacg tgttcgccca agccgacgag gtcttgggct ttcctgtgtc cgcgctgtgc
     3001 ttcgaagggc cggaagccga actgaccgac acgatcaatc agcagccggc cattctgatc
     3061 gacagcgtcg ccgcgctgcg cgtcgtcgaa gcgcgcgcgc cggagacgcg gccagccttt
     3121 ctcgccgggc acagcctcgg cgaattcacg gcgctcgtcg ccgccggaac gcttgcattc
     3181 gcggacgccg tccagttagt gcgcgagcgc ggccgcctca tgaagcaggc cggcgcgctc
     3241 gctcccggcg gaatggctgc catcctgaat ctcgaccgtg agacgctggc cgatgcgtgc
     3301 cgcgaggccg aggccgggac aggcaagcgc gtccagattg cgaacgacaa ctcgcccggc
     3361 cagatcgtta tctcaggcga caaggccgcg ttggacaaag cgatggaact ggcaaaggcg
     3421 aagggtgcgc ggcgcgtcat cccgctggcc gtcagcatcg ccgcgcattc gcacctgatg
     3481 cagccgattg tccgctcgtt ccgaggcgag ctggattcgg tcgcgttcgc tccgatgaag
     3541 attccggttg tcggcaacgt ctacgcccgg ccgatcgact tcgtcgatat tcgcgacgag
     3601 cttgaggcgc agctcaccgc gcccgtgcgc tggacggaga caatccagta tctcgctgcc
     3661 cggggcgtca cgcgcttcgt cgaagtcggg ccgaaggacg tgctcgccgg actggtgcgc
     3721 cgcatcgatc ccagtgtgac ggcgatgagt gtcggcgacc cagcgggcat agaatggctg
     3781 gtggcgtcat gatctcggtt ccccgaatcg gattcatcgg cctcggcctg atgggcaaac
     3841 ccatggccgg ccatctcgtg agggccggct atcaagtcac ggtgcataat cgcagtaggg
     3901 cagccgtcga cgaactcgtc gcgcagggcg ggcgcgcggc ggacaacccc aagggcgtgg
     3961 cggcggagag cgacgtcgtg atcaccatgc tccccgattc gccggacgtt gaagctgtgg
     4021 ctttcggcga acaaggcatc ctggcggccg cgcggccggg gatgatccac atcgacatga
     4081 gcacgatcgc gcctgccgcg gcgcgccgca tcgccgaggc cgagcaccac gccggcgtgg
     4141 agaacctcga tgcgccggtg agcggcggcg acgtcggcgc gaagaacgcg acgctgacga
     4201 tcatggccgg cggcaaaccc gatgtgttcc agaagtgcct gccgattttt gaaaggctcg
     4261 gcaagaaaat cacgctcatc ggcgatcacg gcgcgggaca gatcgccaag gcgtgcaacc
     4321 agatcatggt cgccgcgcag atggtggcga tgggcgagct gctcgtattc gcgcagaagg
     4381 ctggcgccga tccgcgcgaa gtcgtcgagg cgatcaaagc gggcgccgcg caatgctgga
     4441 cgcttgacac caagccgcag cgcttgttcg ccggtaaccg cgcgccgggc ttcaagtcgt
     4501 cgatgatgct gaaagacctg aatatcatcg tcgattcggc gaaggcatac agcatcccgt
     4561 tgccaacgac cgccgtgaac gcgcagttgt ttgccgcgct ggtggccgcc ggccagggcg
     4621 acctggacaa ctcggcagtg atcggcgtga tcgaggagct ggcgcgagtc aagcttttgg
     4681 agattggaga ttagagattg gaagtttgaa gttcgaagtt tgaagtttga gacttggagg
     4741 ggccatgcag ctcaaggaca aagtggccgt cgtgacggga gcctcgcgcg gaatcgggcg
     4801 ggcgatcgcg atagaactgg ccaggcgggg ggcgaaagtc gtcgtcaact acaatgccag
     4861 cgccggtgcg gccggggaag tcgtgagcgt aatcaaagag gcgggcgggg aagcgatcgc
     4921 cgtgaaggcc gacgtgagca ggctcgacga ggcccaggcg ttgatcaagg ccgccaccga
     4981 aacgttcggc cgcctcgaca ttctggtgaa caacgccggc accacgcgcg acatgctgct
     5041 ggcgatgatg aaggaagaag actgggacct ggtgctggcg accaacctga agagcgcctt
     5101 caactgcagc aaggccgcgc tgcgaccgat gatgcgccag aagtacggcc gtatcgtgaa
     5161 cattacgtcg gtcgccggca tcgcgggcaa cccgggacaa acgaattacg ccgcctcgaa
     5221 agccggtctc atcggattca ccaagtcgct ggcgaaagag attggccccc gccatatcac
     5281 agtgaacgcc gtggcgccgg gcttcgtcga aacggagctg acgaaagact tgcccgccga
     5341 tctgaaagaa gcggcgctca aagcgacgcc gctgggccgc tggggaaccg ccgaagagat
     5401 ggcgtatgcc gtcgccttcc ttgcgtccga ccaagcagct ttcatcaccg gccagacgct
     5461 gtcggtcgac ggcggactgg cgatgcagtg agatggcaca ggaggtcacc gcgtggctgc
     5521 tgaaaacgtc tcggaaagcg cgacggcgcc cgacgcaggg gcaggtctcg cgcctgccca
     5581 ccggggacgc gtcacggtcg cgcagcgggt gctcgacgcc atcgcgcaat tgaccacgct
     5641 gaacgtgcct ggcgtggtgc ggctcgccac gcgcggcggg ctgcgtcgag gttcagacgg
     5701 cgtgcgcgtc gacgtcgtcg acgataaggt gttcgtcgac gtctacgtga tcgtgacgca
     5761 cgacgccaac atgcgccaga tcggcaaaga gatacaggtc gagatcgcgc gggcgatgaa
     5821 ccagatcgtc ggcatggacg cggcggaggt gaacgtccac atccaggacg tggagccgcc
     5881 ggccgcctga tcggcaactg gaccgcatga aaggacgacg gcgcgcacgc agcgtcgcgc
     5941 tgcaggccct ttacgaactg gatagcacgc atcacgaccc gggcaccgtg ctggcgcacc
     6001 gcttcagcga cgtgcgcctc aagccggagg gcgaggcgtt cgcccgcgcc ctggtcgccg
     6061 gcgtgctgcg cgagcgcgat cggctcgacg cgctgatcca gcggcatgcg ccggagtggc
     6121 cgatcgacca gctcgccgtc gtcgatcgca acgtgctgcg catcgccatt ttcgagttcg
     6181 acatcgccaa gatcacgccg atgcgcgtcg cgatcaacga agccatcgag ctggccaagg
     6241 aattcggggc ggacagcgcg ccgcgcttcg tgaatggcgt gctgggcgcg atcgccagcg
     6301 cgcccgacgc ttcgacaaag cacgtcgagg ccgcgaagaa gtgacatggg tgagatactc
     6361 ggaatcggga tcgaagagct gatcttcgtc ggcatcctga tcgcgctgct attcgggccg
     6421 gagagcattc ccaagatggc gcgtaccgcc ggccgggccc tcaatcggct gttccgctcg
     6481 ccgctgtacc gggagagcca acaaatccgc aagcagatcc aggacctgcc tgccgccctg
     6541 gcccggctgg ccgagctgga agaaatgcag aaaaacctca acaacgaaat cagcgatctg
     6601 agaagatcga tcgaccctgg cctcagccag actgcgcctt ccacgacaat ttcccctctc
     6661 cagccggcag cgacgacgcc tggcccggac gcgcaggtcg agccgcccac ttcggatgca
     6721 tcgccgcgag agccggacgc cccagaggcc gatcactcca tcgagccgcc gcacgcgccc
     6781 ccgcgcccgg cggccgacga gccatcccat gccactcgtt gatccggcgc tgctcgtcat
     6841 cgcgctgttc gcgttcagct ttctcttttc actgctcgcc atcttgcgaa acctgcgcgt
     6901 catgcgcgcc gaagcggccg aggaagacgg agacctggcc ggcacgctcg agaccgtcac
     6961 ccaggactgg cgcgtgatcc tgccgcacct ggtcgagctg cgcaaccgca tcttctactc
     7021 ggtgatcatg ctcgccgtca cgaccgccat cagtttcacc tttacatcgc aattgctcga
     7081 tgcactgatg gccccgctgc cctccgacgc catcgtcgtc gctgcgacga tcaccgatca
     7141 gttaggcatg ttcatgcgca tcgcgctcac ctcgggcctc gcgctggcca tgccgttcat
     7201 cgccacgcaa ttctggatct ttatctcgca aggtttgaag gcgggcgagc gccgctacgt
     7261 gtactggttc gtgcccggcg cgacgatcct gttcatcacc ggcgcagcct tcgcctactt
     7321 cgtgatgctg ccggcggcga ttcccttcct gttgagcatt ttcgaggacg tgcccaacta
     7381 tctccggctg agcgactacg tggccgacgt gacgcggctg ctgatgtgga tcggcttgag
     7441 cttcgagatc ccgctggtga ttgcggcgct ggcgcgcgtc gggctgatca ccgcgcggca
     7501 gctcgtgcag ggctggcgct tcgccgtcgt cgccatcgcc gtgctggccg cgatcgtcac
     7561 tcccaccgcc gatccggtca acatggcgct cgtcatggcg ccgctgttcg tcctgtacct
     7621 gctcagcatt ctgctcgcgg cgctggtgcg aagggaaaag agatcgcctc gaccgaaacc
     7681 cctatcgaaa cggcgattat cgctgcttaa gaggagaagt cgcacgtgaa gcgcggagcg
     7741 tcagttcacg cttcacgcct cacgctttac gtttcacgct tcacgtttca cagattgctc
     7801 atcacgctca ggagaaactc ctcatgctcc gaacccgcgt cttgatcatg ggtgccgccg
     7861 ggcgcgactt tcacaatttc aacgtgttct tccgcgacaa cgagcgctac gaagtcgtcg
     7921 ccttcaccgc gacgcagatt cccaacatcg aaggtcgcgt ctaccccgac gtgctggccg
     7981 gtaagatgta tccccatggc atcccgatct atcccgaatc cgagcttcgg cggctgatcc
     8041 gggacctgga cgtcgaccag gtggtcttcg cttactcgga tatcagccac gagcacgtca
     8101 tgcacaaggc gtccgaagtg ctggccgcag gggccgactt caggctgatg ggactgaagg
     8161 cgacgcagat caagtcgagc aaaccggtcg tgtcgatcgg cgccgtgcgc accggctcgg
     8221 gcaagagcca gacgacacgc cgcgtcacgc aggttctgaa ggagctgggc tacaccgtcg
     8281 cggcgattcg ccaccccatg ccctacggca acctggtcaa gcagacggcg cagcgcttcg
     8341 ccgactacga cgatctggac gagtacgact gcacgatcga agagcgcgag gagtacgagc
     8401 cacacatcga tcgcggcgtg accgtgttcg ccggcgtcga ctacgggcgc gtcctggaga
     8461 tggccgaggg ggaagccgac atccttgtgt gggacggcgg caacaacgac ctgcccttct
     8521 acaagagcga cctgcacatc gtcgtcgtcg atccacaccg gcccggccac gaaacggcgt
     8581 accaccccgg cgaagcgaac ctgcgcgcgg ccgacgtcgt cgtgatcaac aaaatcgaca
     8641 ccgccgattt caagaacgtc gccgccgtgc gcgagaacgt ctatgcagtc aatccaactg
     8701 cgcagatcgt ggacggcgcg tcgccgatat tcgtcgacga tccgggcgcg attcgcggca
     8761 agcgcgtgct cgtgatcgaa gacggcccta ccctcaccca cggcgagatg gcttacggcg
     8821 caggcgtgat cgccgcgcag cgcttcggcg cgaaggagat cgtcgatccg cgcccctacg
     8881 ccctcggctc gatcctcgat acctaccgga agtacccgac gaccggcagc gtcctcccgg
     8941 ccatgggcta cggcgagcgg cagaccaaag aactggaaga gacgatcaac ggcacaccgg
     9001 ccgacatggt gatcatcgcc acgccgatcg acctgcgccg cat
//
LOCUS       DBNHXB010000110         3804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2202078, whole genome shotgun sequence.
ACCESSION   DBNHXB010000110 DBNHXB010000000
VERSION     DBNHXB010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2202078"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="AC162G_04285"
     CDS             complement(<1..296)
                     /locus_tag="AC162G_04285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5075064.1"
                     /translation="MSAQEWLSALAQLYPPDRLLAQPAQLASYESDALTAYRSRPAAV
                     VLPETQNEVVETVRLCHRFRVPFVARGSGTSLSGGSLPVKDGILIALNRLNRILR"
     gene            complement(422..1327)
                     /locus_tag="AC162G_04290"
     CDS             complement(422..1327)
                     /locus_tag="AC162G_04290"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5075065.1"
                     /translation="MRLSGLHLLLTYQCTLECDHCFVWGSPWQTGTMTLHDIRRIMEQ
                     ANDLGTITSIYFEGGEPFLYYGTLLKGVQMASHLGFQVGIVSNSYWATSVEDAVEWLR
                     PFAGLLQDLSISSDVYHWSEEVSRQSQNAQAAARQLGIPIGVMSVAQPEATNAASVVG
                     QLPIGESAVMYRGRAAEKLVSQAIRHPWTQFTACLDEDLRDPGRVHVDPLGNVHICQG
                     IAIGNLFRTPLKEICETYDPDAHPIVGPLLRGGPVELVRQYGLSHAETYADECHLCDE
                     ARRMLRARFPEILMPDQMYGVPEGN"
     gene            complement(1381..2343)
                     /locus_tag="AC162G_04295"
     CDS             complement(1381..2343)
                     /locus_tag="AC162G_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020634844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075066.1"
                     /translation="MKRDHPTIQWLLESGDPSIRYFALTDLLGKTHRSPEVRAARDQI
                     VHGPRARKLLAGQKADGGFGVHPYQKWTGAHWRLVSLVELGIPAGEPRAVAAADQVLR
                     WLTGDAHRRRIKRIDGLTRRCASQEGNALAVCSRLGMAGDPRVKHLAQSLLEWQWPDG
                     GWNCDKDPHARHSSFYESLAPLWGLIEYQRATGNADAGKAAGCTAEFFLQHRLFRSDK
                     SGEVIDAEWLQLHYPLYWHYDVLQGLLILSRMGPLTDPRLSEALDAVEAKRLPDGRWK
                     PEGYYWWPPGKRASNVEVMDWGRHGPNPMITLNALRVLSAGGRL"
     gene            complement(2410..>3804)
                     /locus_tag="AC162G_04300"
     CDS             complement(2410..>3804)
                     /locus_tag="AC162G_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5075067.1"
                     /translation="RVDRARLSPAHADLLKHMAFINRPPYEGGSWHHHFAFGYLLADP
                     GLYCSLIVTNQTAYAIHKYAPEHRRWLEPLLSGALWGATWMTETQGGSDLGANTTIAR
                     LEDGGWRLYGEEKYFASNAGLADLAIVTARPQGAPPGPKGIALFLVPRLDQDSRLNFH
                     VRRLKEKSATRAVPTGEVEFHGSQAFLIGDAEMGIYYTLENLTVSRLANAVGAMGLAR
                     KAHLESLFRVQARAAFGKPLVEHPLIRRDLTDLAVRLAGGSSLVFHAIQAFDAAWHET
                     PPYTDRYHYARFLSHLVKNRTAEHAAHATLLAMELFGGLGFLDEYVVSRLHREALVTL
                     IWEGTSNIQALDMLEAMRKKAAHESFLDEVVPMLERAGTDESALAQKALDEVLAHLGS
                     LEPEEAQWYGKDALATLADIAQVALLYDLADHAGERYAKLAALYARRFLADAPYPGWA
                     LADREVWSPIDLSA"
BASE COUNT          667 a         1214 c         1222 g          701 t
ORIGIN      
        1 cgcaggatgc ggttgagccg gttgagcgcg atgaggatgc catccttcac cggcagcgat
       61 ccgccggaca ggctcgttcc gctgccgcgt gcgacgaacg gcacgcgaaa gcgatggcac
      121 aggcgcacgg tctcgacgac ttcgttttga gtctcaggaa ggacgacggc cgcgggccgg
      181 ctgcggtagg ccgtcagcgc gtcggactcg tacgacgcga gctgggccgg ctgtgccagc
      241 agccggtcag gaggatacag ttgtgccagt gcagacagcc attcctgtgc gctcatagac
      301 accgcctttc aaatcccaaa cttcaaactc caaacttcaa aataagggtc aaggatcaga
      361 tatagtgtag caccgaaggg gccagaaatc caaatggttt ccctcagttc cctcaggccc
      421 ctcagttccc ttcaggcact ccatacatct ggtcgggcat caggatctcg gggaatcgtg
      481 cgcgcagcat gcgccgggcc tcgtcgcaga ggtggcattc gtcggcgtag gtctccgcat
      541 gcgaaagccc gtattggcgc accagctcca caggtccacc tctgagcaag gggccgacga
      601 tcggatgcgc atccggatcg tacgtctcgc agatctcctt cagaggcgtg cggaacaggt
      661 tgccgatggc aatgccttga cagatgtgca cgttcccaag cgggtccacg tgcacgcgcc
      721 ccggatcgcg caggtcttcg tccaggcatg cggtgaactg tgtccatggg tgacggatcg
      781 cctgcgagac gagtttctcg gcggcccgcc cccggtacat gacggccgac tcgccgatcg
      841 ggagctggcc gacgacggat gcggcattcg tcgcctccgg ctgggcgacg ctcatgacgc
      901 cgatcgggat gccgagctgt cgtgccgctg cctgtgcgtt ttgcgactgc cggctgacct
      961 cttcgctcca gtggtacaca tcgctgctga tcgagagatc ctgaagcagt ccggcgaagg
     1021 gcctcagcca ttcgacggcg tcttcgacgc tcgtcgccca gtagctgttc gagacgatcc
     1081 cgacctggaa gcccagatgc gacgccatct gaacgccttt gagcagagtg ccgtagtaaa
     1141 ggaacggctc tccgccctcg aagtagatcg atgtgatcgt gccgaggtcg ttggcttgtt
     1201 ccatgatgcg ccgaatgtcg tgcagcgtca tcgtgcccgt ctgccacggg ctgccccaca
     1261 cgaagcagtg gtcacactcg agcgtgcact ggtacgtcag cagcagatgc aatcctgaga
     1321 gtctcatttc ggtgtccttt cacccttgcg aaggttgcga accttcgcaa gggttgtcga
     1381 ctacagccgg cctcccgcgc tgagaacgcg cagcgcgttc agggtgatca tcggattggg
     1441 gccatgacgt ccccagtcca tgacttcgac attcgaggcg cgcttgccag gcggccacca
     1501 ataatagcct tcgggcttcc atctgccgtc cggtaggcgc ttcgcctcga cagcgtccag
     1561 cgcctcactc agcctcggat cggtcagcgg ccccattcgc gacaggatga gcaggccctg
     1621 gagaacgtcg taatgccaat acagcggata gtgcagttga agccactcgg cgtcgatcac
     1681 ctcgccactc ttgtcggagc gaaacaggcg atgctgaagg aagaattcgg ctgtgcagcc
     1741 cgctgccttt ccggcatccg cattgccggt cgcgcgctgg tattcgatca gtccccacag
     1801 cggcgccagg ctctcgtaga acgacgagtg ccgggcgtgt gggtccttgt cgcagttcca
     1861 gccgccgtcc ggccattgcc attccagcag cgactgtgca agatgtttga cgcggggatc
     1921 gcccgccatt cccagccggc tgcatacggc cagggcgttc ccttcctgcg acgcgcagcg
     1981 gcgcgtcagc ccgtcgatgc gcttgatgcg gcggcgatgt gcgtcgccgg tcagccagcg
     2041 caagacctga tcggcggcgg cgacggcgcg cggctcgccg gcagggatgc ccaattcgac
     2101 gagcgacacg agccgccaat gtgcgccggt ccacttttga tacggatgca cgccaaagcc
     2161 gccgtcggcc ttctgcccgg cgagcagctt gcgtgcgcgg ggtccgtgca cgatctgatc
     2221 gcgggcagcg cgcacctccg gcgatcgatg cgtcttgccg agcaggtcgg tcagcgcgaa
     2281 gtaacggatc gacggatcgc ctgattcgag cagccattga atggtggggt gatcgcgctt
     2341 catgagtctc catccaagac ttcggaagtc tttaagactt ccgaagtctg aaaggcgggc
     2401 tggttcacgt catgcgctca gatcgattgg agaccacacc tcacggtcgg cgagcgccca
     2461 gcctggatac ggcgcgtcag ccaggaagcg ccgggcgtac agcgcagcca gcttggcata
     2521 gcgctccccg gcatgatccg ccaggtcata aagaagcgcc acttgagcga tgtctgccag
     2581 ggtcgccagg gcgtccttgc cgtaccactg cgcttcttcc ggctcgagcg atcccaggtg
     2641 cgcgagaact tcgtccagcg ccttctgcgc gagagccgac tcgtccgtac cggcgcgttc
     2701 cagcatcggg acgacttcgt cgaggaaact ctcgtgcgct gcctttttgc gcatggcctc
     2761 gagcatatcc agcgcctgga tgttgctcgt gccttcccag atcagtgtga cgagcgcctc
     2821 gcgatgcagc cgcgacacga cgtattcgtc caggaagcca agcccgccga aaagctccat
     2881 cgccagaagc gtcgcgtgcg ccgcgtgctc ggcggtgcgg ttcttcacca ggtgggagag
     2941 aaagcgtgcg taatggtacc ggtcagtgta gggcggagtt tcgtgccagg ctgcatcgaa
     3001 tgcctgaatc gcatggaaga cgaggctcga tccgcccgcg aggcgcacgg cgagatcggt
     3061 caggtcgcgg cggatcaacg gatgctcgac gagcggcttg ccgaacgccg cgcgcgcctg
     3121 cacgcggaac agcgattcca ggtgcgcctt gcgcgccagc cccatcgccc cgaccgcatt
     3181 cgccagccgg gagacggtca ggttctccag cgtatagtag atgcccatct ccgcgtcacc
     3241 gatcaggaat gcctggctgc cgtggaactc gacctcgccg gtgggcacgg cgcgcgtggc
     3301 gcttttctcc ttcaagcgac ggacgtggaa gttgagccgg ctgtcctggt cgaggcgcgg
     3361 cacgaggaac agggcgatcc ctttcgggcc gggcggcgct ccctgcggcc gcgccgtgac
     3421 gatcgccaga tcggccagac ctgcattact cgcgaaatac ttctcttcgc cgtacagccg
     3481 ccagccgccg tcttcgaggc gagcgattgt cgtgttcgct cccaggtcac tgccgccctg
     3541 ggtttcggtc atccaggtcg ctccccacag ggcgccggag agaagcggct ccagccagcg
     3601 cctgtgctcg ggggcgtatt tgtgtatggc gtaggctgtt tggttggtga cgatcagcga
     3661 gcagtaaagc cctggatccg ccagcaagta gccgaacgcg aagtgatggt gccacgagcc
     3721 gccttcgtag ggcgggcgat tgatgaaagc catgtgcttg agcaggtcgg cgtgggccgg
     3781 gctgagccgc gcccgatcca cgcg
//
LOCUS       DBNHXB010000111        10914 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_692502, whole genome shotgun sequence.
ACCESSION   DBNHXB010000111 DBNHXB010000000
VERSION     DBNHXB010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10914)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10914)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10914
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_692502"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..595)
                     /gene="hpf"
                     /locus_tag="AC162G_04305"
     CDS             complement(35..595)
                     /gene="hpf"
                     /locus_tag="AC162G_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR5075068.1"
                     /translation="MEVTIRGKNIVINDRINDYVTKKLTKLDRYLPTIDEAHMDLSVE
                     KTRSAQDRHVAQLTVRNRGALLRAEERDQDIFAAIDAVLDKMQRQISRYKERRTQRGE
                     RAAGEKPNVAAVEAAAEQDAEPLPGDIVRIKRFAVSPMHREEALEQMELLGHDFFVFF
                     DAETESFSVLYRRKDGNYGLLQPELA"
     gene            complement(627..1334)
                     /locus_tag="AC162G_04310"
     CDS             complement(627..1334)
                     /locus_tag="AC162G_04310"
                     /inference="COORDINATES: protein motif:HMM:NF039185.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HIR5075069.1"
                     /translation="MAEFTFSPVARWRRIVDLIFPPRCGGCGAPGSLWCAACQASAQP
                     IAPPFCARCGKPGTPSHLCASCRASAPAIDSIRALTMFDGRMRHAIHAFKYQGLATLA
                     DPLGDALASFWLAAPAPARAIAPVPLHPQRQRERGYNQAELLARRVGRKADLPIYAGA
                     LRRVRATAVQMTLGAEERRANVAGAFRCSTDVLRGAAVLLIDDVCTTGATLDACALAL
                     KEGGVTAVYGLVLARAA"
     gene            complement(1410..2336)
                     /locus_tag="AC162G_04315"
     CDS             complement(1410..2336)
                     /locus_tag="AC162G_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR5075070.1"
                     /translation="MGFLGIGLLVAIGLAVVLILVGLSARDPREALQARLAEYSAREQ
                     AATLEEIELSMPFSERVIAPLVRSMAEFVKRFTPERTLEATRHKLDLAGNPQNWSASE
                     FFGVRVLAAIVLAALIFVVLWLTNAEIGMRLLMTIVFALLGFFLPVLFLGSRIRSRQS
                     NIVKSLPDALDLLTVCVEAGLGFDQAMKKVSEKWNNELSRAFTRVLQEIQLGRMRREA
                     MRDMANRMEVSEVTSFVAAIIQAEVLGVSMTKILRIQADQMRTRRRQRAEEKAHQASI
                     KMIIPMVFLIFPAIWVVLLGPAILQVMQAGIF"
     gene            complement(2355..3320)
                     /locus_tag="AC162G_04320"
     CDS             complement(2355..3320)
                     /locus_tag="AC162G_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR5075071.1"
                     /translation="MSPQIIALAIGGVALVILLFGVISSLLPRRELARRLERFATVEI
                     GAGQPAQEARARMSLVGDSINKALAGRSFADDIATQLARADLKLTVFEYLIFLVASVV
                     VGGVLGFLLSSGSIVFALLIAVVGFFVPRTLVNIAQGRRLRAFNNQLGDFLNMMVNGL
                     RAGYSATQAMEAVASELSPPLATEVGRVVQEMQLGLTQEQALANMLRRVRSDDLDLII
                     TAMNVQREVGGNLAEILDTISYTIRERVRIKGEISVLTAQGRISMYVIVFLPFAITGF
                     LYLINPTYISQLFTTGFCGWSMVVCSGLSILTGYFIIRRIVSIEV"
     gene            complement(3338..4771)
                     /locus_tag="AC162G_04325"
     CDS             complement(3338..4771)
                     /locus_tag="AC162G_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HIR5075072.1"
                     /translation="MSLLKRIEKGQPQTPQTPAPGQSGGSAARSGPPAPPESSKLAEM
                     RMRRPTIAASPVRDAYLELKTRVQTKLLAELDPSMDVTKTDEVRASIEELFDSVLTEE
                     NIILSRVERSRLFEAIVAEILGFGPIEPFIADETITEIMVNGPKSIYIEREGRIERTS
                     ATFENADHVMRIIDRIVAPLGRRIDESSPYVDARLPDGSRVNAIIPPLSLVGPVITIR
                     KFFKIPLTIERLIELGTVSPEAVEFMKACVQGKCNILVSGGTGSGKTTLLNILSSFIP
                     EDERIVTIENAAELQLRQEHVVTLESKPPNIEGRGEVTIRDLVINSLRMRPDRIIVGE
                     VRGAEALDMLQAMNTGHEGSMTTLHANSPRDALARMETMVLMAGMDLPHRAIREQISS
                     AIDVIVQEERMRDGSRKIVSINEVQGMEGEVITMSSLFEFEKIGIENGKVIGRFKPTG
                     LRPKFMEKIEASGIRLPPNIFGLGQRR"
     gene            complement(4795..6078)
                     /locus_tag="AC162G_04330"
     CDS             complement(4795..6078)
                     /locus_tag="AC162G_04330"
                     /inference="COORDINATES: protein motif:HMM:NF022077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE/TadZ family protein"
                     /protein_id="HIR5075073.1"
                     /translation="MGSDKIRVLIVDDIPETRENVRKLLAFESDIEVSGVAGTGHDAI
                     QSAREYQPDIVLMDINMPGLDGISAVEAITQEVPTVQVIMMSVQSDTDYVRRSMLAGA
                     RDFLTKPFSSDELVSTIRRVYRMGQQRAAAMPPQAMVQMVGPSGRRMAVPAAVAAPEG
                     SVITVYAPKGGVGTSMVAVNLAVALQRPDAKVALIDASLQYGNVDVMLNLHVSRSIAD
                     ISQSINDLDADLLSTVMTPHPSGIKTLLAPNRPELADLVQPDHLQRIVDEMRLQFDYI
                     VIDTASTLSDLTLTALDIADRVVLLTVAEVPAIKNARVFFQVTEALGYPSQKMMLVLN
                     QMDSRNPITAKLIEDNLKHKVLLQIPHDADTVDQSIRRGLPLVTEQRNKPIAAAINQL
                     AERLAADLKASREAAVEEAALEDASRKRGARFFRQ"
     gene            complement(6167..7219)
                     /gene="cpaB"
                     /locus_tag="AC162G_04335"
     CDS             complement(6167..7219)
                     /gene="cpaB"
                     /locus_tag="AC162G_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HIR5075074.1"
                     /translation="MRRSRTLILLGVVMLLAAVAAYILLGPGNIGQPPTPEVTVEPTP
                     AAPMVEVVVAAQNIPRGRLITADSVTMASLPASNVPPGAFTNVEDVQNFIARTDILYN
                     QPLMDVMLTRDPTLLAARGSDAALLIPNDRRAVALPVDQLSSVGYAIRPGDHVDVLAS
                     FWLVDVDRDGQYKTIFFNRNLVDELIAAGLEPAAAAQAVLASLQSATVFPRLSSQLVL
                     QDLEVLSLGDWVDPTPIPRFTPGAPGEPTPTTVSVPGATPTATPPRPNVIILIVDPQQ
                     ALILQWLRESDAIIDLALRGAPDRAPVSTTSVTLQYVFDSFSVTLPPKLDFAINYPPP
                     PNEAICPERWTKDVCR"
     gene            complement(7266..8000)
                     /locus_tag="AC162G_04340"
     CDS             complement(7266..8000)
                     /locus_tag="AC162G_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR5075075.1"
                     /translation="MRDRLALGVAAAFALLVAVSSLGFRPSPHSRLEQVPPSPTPTQV
                     VISTPTSTPVRPTSTLVPPTSTSAAPTSTSTVVATGVPPQPTTTRGAPRPPGPPATPS
                     LGIRVNACARVVGAQGLILGDAPGFGANHVQIVGRGDVVLVTAGPERADGVWWWRVAT
                     RTGVVGWGSNDQLLPQSGACFELATATALATPPPTAMAGITATPGGQAGSLPSTGTSA
                     GLVTAGILILILVVVGVIRRRTQSAT"
     gene            complement(8159..9223)
                     /locus_tag="AC162G_04345"
     CDS             complement(8159..9223)
                     /locus_tag="AC162G_04345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5075076.1"
                     /translation="MDALHGRAAGSGSSSHLLDELKAEYEQTQRELKEIDLLIQQTGG
                     EVDRLAQRNAQAANRVKQTEIHIDTVPRSDLQAVYAEAQEAQKRLVMMKSQLEKLQSD
                     QQNLSRLANALNRSVSAVSDGAGVVAPGVGIDSGAADAGQPAIVRIIETQEQERQRLV
                     RQLHDGPAQSLTNLILQAEICERLFDHDAKRARSELSNLKTAVTSTFQKVRDFMFDLR
                     PMMLDDLGLVPTLKRYVDGCAEKSGMPIALTVTGNERRFTGYKEMALFRIVQDLLGNV
                     RQHAHATRAQVFLDLTEDAVRVSVEDNGSGFDVVETLSARKGIGLSTLRERVEMMGGQ
                     VQIDSTPGRGTKVSLILPLP"
     gene            complement(9259..10002)
                     /locus_tag="AC162G_04350"
     CDS             complement(9259..10002)
                     /locus_tag="AC162G_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5075077.1"
                     /translation="MQQKITVLIVDDHPLFRQGLREVLSAEPDLVVVGEASDGEEALR
                     AARELSPRVVLVDVNIPTLNGLQVARRLRQDAPGARAVILTGYDDEEQMFHAIKAGAH
                     GYFPKDVEPHKLVEAVREVNVGRYIVSGLVMDDAQLAPWLYKQSERFGIVEGELGDEA
                     FVPLSPREMEILQHITRGLSNKEIAHQLGISHQTVKNHMTSILRKLNVEDRTQAAVYA
                     LRRGWVRLQDTQVGQGDRLGLRPSLSKDP"
     gene            complement(10115..10579)
                     /locus_tag="AC162G_04355"
     CDS             complement(10115..10579)
                     /locus_tag="AC162G_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075078.1"
                     /translation="MDRLSRERHRIARPVWVGSVIAATAAFWLVGAGAYRVDVNVIEN
                     ELVASARWIESNTPPGALVAAHDIGAIGYFSRRPLLDLAGLVSPEVIPIIRDEAALLA
                     LLRSRAADFVAAPPNFYSAALLDAPELEPAFEGDSPFAPKHWLIFRARWGRP"
BASE COUNT         1860 a         3652 c         3495 g         1907 t
ORIGIN      
        1 gagatccccg cgccgcgccg agacgaaccg atttctaggc tagttccggc tgcagcaggc
       61 cgtaattgcc gtccttgcgc cggtatagca cgctgaacga ctcggtctct gcgtcgaaga
      121 acacgaagaa atcgtggccc agcagctcca tctgttccag cgcctcttcg cggtgcatcg
      181 gcgacacggc gaagcgcttg atgcgcacga tgtctcccgg cagcggctcg gcgtcctgct
      241 cggcggccgc ctcgacggcc gccacgttgg gcttctcgcc ggcggcgcgc tcgccgcgct
      301 gggtgcgccg ctccttgtag cgcgaaatct ggcgctgcat cttgtccaac accgcgtcga
      361 tcgcggcgaa gatatcttga tcgcgctcct cggcgcgtag gagtgcgccg cggttgcgca
      421 ccgtcagttg cgccacgtgc cgatcctgag cggatcgggt cttctctacc gagaggtcca
      481 tatgcgcttc gtcgatcgtc ggcaggtagc gatcgagctt ggtcaacttc ttggtgacgt
      541 agtcgttaat ccggtcgttg atgacgatgt tcttgccgcg aatggtgact tccatcccat
      601 cctccggtac gtgatttgag cttgcgtcac gccgcgcggg ccagcaccag cccgtagacg
      661 gcggtgacgc ctccctcctt caatgccagg gcgcaggcgt ccagcgttgc gccggtcgtg
      721 cacacatcgt cgatcagcag cacggcggcg ccgcgcaaga cgtccgtgct gcatcgaaac
      781 gcgcccgcca cgttggcccg ccgttcctcc gcacccagcg tcatctggac ggccgtggcg
      841 cgcacgcgcc gcagcgcgcc ggcgtagatc ggcaggtcgg ccttgcggcc gacgcgccgc
      901 gccagcagtt cggcttgatt atatccccgt tcacgctgcc gctgtgggtg cagcggcacg
      961 ggcgcaatcg cgcgcgcggg cgccggcgcc gccagccaga atgaagccag ggcatccccc
     1021 agcggatcgg ccagggtggc caggccctga tacttgaacg cgtgaatggc gtggcgcatg
     1081 cggccgtcaa acatcgtcag cgcccggatc gaatcgatcg ccggcgctga ggcgcggcag
     1141 ctcgcgcaca gatgggatgg cgtgccgggc ttgccacaac gggcacagaa gggaggggcg
     1201 atgggctgcg cgctggcctg gcaggctgca caccacagcg agcctggcgc gccgcagccg
     1261 ccgcaacgcg gcgggaagat gagatcgacg atgcggcgcc aacgagcgac aggagagaac
     1321 gtgaactcag ccaccgctgg gccggtcctt ctccggtttt ggtcccctcc ccctgcggcg
     1381 cggggggagc cggagggggg cgaggcgcgc tagaatatac ccgcctgcat gacctgcagg
     1441 atagccgggc cgagcagcac cacccagatc gccggaaaga tcaggaagac catcgggatg
     1501 atcatcttga tcgacgcctg gtgcgctttt tcctcggcgc gctggcggcg gcgcgtgcgc
     1561 atctggtccg cctggatgcg caggattttc gtcatgctca cgcccaggac ttccgcctga
     1621 atgatggcgg ccacgaagga agtgacttcc gacacttcca tgcggttcgc catgtcgcgc
     1681 atcgcttcgc gccgcatgcg gccgagctgg atctcttgca gcacgcgcgt gaaggcgcgg
     1741 ctgagctcgt tattccactt ctcggatacc ttcttcatcg cctggtcgaa gcccaggccg
     1801 gcctctacgc aaaccgtcag caagtcgagc gcgtccggca gcgacttgac gatgttcgac
     1861 tggcgcgacc ggatccggct gcccagaaac agcaccggca gaaagaagcc caacaaggcg
     1921 aagacgatcg tcatcagcag ccgcatgccg atctcggcgt tcgtgagcca cagcaccacg
     1981 aagatcagag ccgccagcac gatggcggcc agcacacgca ccccgaagaa ttccgacgcc
     2041 gaccagttct gcggatttcc ggccaggtcg agcttgtgcc gggtagcttc cagcgtgcgc
     2101 tccggcgtga agcgcttgac gaattcggcc atactgcgca ccagcggcgc gatgacgcgc
     2161 tcgctgaacg gcatcgaaag ctcgatctct tcgagcgtcg cggcctgctc gcgcgcgctg
     2221 tactcggcca gccgcgcctg cagcgcctcc ctcggatcgc gcgcgctcag cccgacgagg
     2281 atcaagacca cggccaaccc gattgcgacg agcagtccaa tccccagaaa ccccatcacg
     2341 cactccttga ccggttatac ctcgatgctg acgattctgc gaataatgaa gtaacccgtc
     2401 agaatgctca acccgctgca cacgaccatc gaccagccgc agaaacccgt ggtgaacagc
     2461 tgcgagatat aggttgggtt gatcaggtac aagaagcccg tgatggcgaa cggcaggaac
     2521 acgatgacgt acatcgagat gcgcccctgg gcggtgagca cgctgatctc acccttgatg
     2581 cgcacccgct cgcgaatggt gtagctgatc gtgtcgagga tctcggccag gttgccgccc
     2641 acctcgcgct gcacgttcat cgccgtgatg atcaggtcca ggtcgtcgct cctgacacgg
     2701 cgcagcatgt tcgccaacgc ctgctcctgg gtcaggccga gctgcatctc ctgcaccacg
     2761 cgccccactt cggtcgcgag cggcggcgaa agctcgctgg ccacggcttc catggcctgc
     2821 gtcgcgctgt agccggcgcg caagccattg accatcatgt tgagaaaatc gccgagctga
     2881 ttgttgaacg ccctcaggcg gcgaccctgc gctatgttga ccagcgtgcg cggcacgaag
     2941 aacccgacga cggctatcag cagcgcgaag acgatcgagc ctgacgacag gaggaaaccg
     3001 agcacgccgc ccacgacgac cgacgcgacc agaaagatca ggtactcgaa gacggtcagc
     3061 ttgagatcgg cgcgggcgag ctgcgtggca atgtcgtccg cgaagctccg gccggcgagc
     3121 gccttgttga tcgagtcgcc gaccagcgac atgcgcgcgc gcgcttcctg ggccggctgg
     3181 cccgccccta tctcgacggt ggcgaaacgc tccaggcgcc gcgcgagctc gcgccggggc
     3241 agcagcgaag agatcacgcc gaagagcaga atgaccagcg ccacgccgcc gatggcgagc
     3301 gcgataatct gtggggacat gctcccgccc tcgctcgtta gcggcgctgc ccgaggccga
     3361 agatgttcgg cggcaggcga atgccggagg cttcgatctt ttccatgaac ttgggacgca
     3421 ggccggtcgg cttgaagcgc ccgatgactt tgccgttctc gatgccgatc ttctcgaact
     3481 cgaacagcga cgacatcgtg atgacttcgc cttccatgcc ctgcacttcg ttgatgctca
     3541 cgatcttgcg cgagccgtcg cgcatgcgct cctcctgcac gatcacgtcg atggcggacg
     3601 agatctgctc gcggatggcg cggtgcggca gatccatgcc ggccatgagc accatcgtct
     3661 ccatgcgcgc cagcgcgtca cgcggcgagt tggcgtgcag cgtcgtcatc gatccctcgt
     3721 ggccggtgtt catggcctgc agcatgtcca gcgcttccgc gccgcgcacc tcgccgacga
     3781 tgatgcggtc cgggcgcatg cgcagcgagt tgatgaccag atcgcggatg gtcacctcgc
     3841 cgcggccttc aatgttcggc ggcttcgact ccagcgtcac gacatgctcc tggcggagct
     3901 gcagctcggc ggcgttttcg atggtcacga tgcgctcgtc ctcggggatg aacgacgaca
     3961 ggatgttgag cagcgtcgtc ttgcccgagc cggtgccgcc cgacaccagg atgttgcatt
     4021 tcccctgaac gcacgccttc atgaactcca cggcctccgg cgagaccgtg cccaactcga
     4081 tcaggcgctc gatcgtcagc gggatcttga agaacttacg aatcgtgatc accgggccga
     4141 cgagcgacag cggcgggatg atcgcgttga cgcggctgcc gtcgggcagg cgcgcgtcga
     4201 cgtacgggct ggactcgtcg atgcgccggc cgagcggcgc gacgatccgg tcgataatgc
     4261 gcatcacgtg atccgcgttc tcgaacgtcg cactcgtgcg ctcgatccgg ccctcgcgct
     4321 cgatgtagat gctcttcggg ccgttgacca tgatctcggt gatcgtctcg tcggcgatga
     4381 acggctcgat cgggccgaag cccagaatct ccgcgacgat cgcctcgaac aggcgcgagc
     4441 gctcgacgcg gctgaggata atgttctcct cggtcagcac cgagtcgaac aactcctcga
     4501 tcgaggcgcg cacctcgtcc gtcttcgtca cgtccatcga cgggtccagc tcggccagga
     4561 gcttcgtctg cacccgcgtt ttgagctcca ggtaagcgtc gcgcaccggc gatgcggcga
     4621 tcgtcggccg gcgcatgcgc atctcggcga gcttggacga ctcgggggga gccggcggcc
     4681 cgctgcgcgc ggcgctgccg ccggactgcc ccggcgccgg cgtttgcggc gtttgcggct
     4741 gacctttctc gattcgcttc aagagtgaca tgctctatcc tcctcacgcg tcgcttattg
     4801 gcggaagaag cgcgcgccgc gcttgcgcga cgcgtcctcc aaggccgcct cctcaacagc
     4861 cgcctcccgc gacgccttca ggtcggctgc cagtcgctcc gcgagctggt tgatcgccgc
     4921 ggcgatcggc ttgttgcgct gctcggtgac cagcggcaag ccgcgccgga tcgactggtc
     4981 caccgtgtct gcatcgtgcg gaatctgcaa cagcaccttg tgcttgagat tatcctcgat
     5041 gagcttggcc gtgatggggt tgcgcgagtc catctggttg agcaccagca tcatcttctg
     5101 cgacggatat cccagcgcct cggtgacctg gaagaacacg cgcgcgttct tgatggcggg
     5161 cacttcggcc accgtcagca ggacgacccg atccgctatg tccagcgcgg tcagcgtcag
     5221 atcgctgagc gtggacgccg tgtcgatgac gatgtagtca aactgcagcc gcatctcgtc
     5281 cacgatgcgc tgcagatggt ccggctgcac caggtcggcc agctcgggcc ggttcggcgc
     5341 gagcagcgtc ttgatcccgg agggatgcgg cgtcatgacc gtggacagca ggtcggcgtc
     5401 gagatcgttg atcgactgcg agatgtcggc gatgctccgg ctgacgtgca ggttgagcat
     5461 cacgtcgacg ttcccgtatt gcaggctggc gtcgatgagg gcgaccttgg cgtcgggccg
     5521 ctgcagcgcg acggccagat tgaccgccac catgctcgtg ccgacgccgc ccttcggggc
     5581 gtacacggtg atgaccgagc cttccggcgc ggccacggcg gccggcaccg ccatgcgcct
     5641 gcccgacggg ccgaccatct gcaccatcgc ttgcggcggc atggcggccg cccgctgctg
     5701 gcccatgcga tagacgcggc ggatggtcga caccagctcg tcgctgctga acggcttggt
     5761 cagaaaatcg cgcgcgccgg cgagcatcga gcggcgcaca tagtccgtgt cgctctgcac
     5821 cgacatcatg atcacctgaa ccgtcggcac ttcctgagtg atcgcctcga ccgcgctgat
     5881 gccgtccaga ccgggcatgt tgatgtccat caagacaata tcgggctggt attcccgcgc
     5941 cgactggatg gcgtcgtgac cggtgccggc cacgccgctc acctcgatat cgctctcgaa
     6001 cgcaagcagc ttgcggacgt tctcgcgcgt ctcgggaatg tcgtccacga tcagcacgcg
     6061 gattttgtcc gatcccacgt tgacgctcct cagcttcagt gagttccttc agcgccctga
     6121 gttcccactc ggcacttgag gaattgaagg aactaaagga actccattaa cggcagacat
     6181 ctttcgtcca gcgctcgggg cagatcgctt cgttcggcgg cggcgggtag ttgatcgcga
     6241 agtccagctt cggcggcagg gtcacgctga agctatcgaa cacatactgg agcgtcacgc
     6301 tggtcgtgct cactggcgcg cgatcgggcg ccccgcgcag ggccagatcg atgatcgcgt
     6361 cgctctcgcg tagccactgc aggatcagcg cctgttgcgg atcgacgatc aggatgataa
     6421 cgttggggcg cggcggcgtc gcggtcggcg tcgcgcccgg caccgacacc gtggtcggcg
     6481 tcggttcccc cggcgcgccc ggcgtgaagc gcgggatcgg cgtcggatcg acccagtcgc
     6541 cgagactcag cacttcgagg tcctgcaaaa caagctgcga cgacagcctc gggaaaaccg
     6601 ttgcgctttg aaggctggcc aacaccgcct gcgcggccgc cgcgggctcg agcccggcgg
     6661 cgatcagttc gtcgaccagg ttgcgattga aaaaaatcgt cttgtactgc ccatctcgat
     6721 cgacgtcgac cagccaaaac gaagcgagca cgtcgacgtg atcgccgggc cggatagcgt
     6781 acccgacgct cgacagttga tcgacgggca gcgcgacggc gcgcctgtcg ttcggaatga
     6841 gcagggccgc gtcggagccg cgtgcagcca agagcgtggg atcgcgcgtc agcatcacgt
     6901 ccatcaacgg ctggttgtag agaatatcgg tccgggcgat gaaattctgg acatcctcta
     6961 cattcgtgaa agcgcccggc ggcacgttgc tcgccggcaa gctcgccata gtcacactgt
     7021 ctgccgtgat cagtcgccca cgcggaatgt tctgcgcggc gaccacaacc tcgaccatcg
     7081 gggcggcggg cgttggctcc accgtcacct ccggcgtggg cggttggccg atgtttcccg
     7141 ggccgagcaa gatatacgca gccaccgcag ccaggagcat caccacacct agcaggatta
     7201 acgtgcgact acgtcgcatg gaaagcctcc tcacaagagt tgggcgatgc cgctgtgccc
     7261 tcgattcaag tcgcgctctg agtccttcga cggatcacgc cgacaaccac cagaatgaga
     7321 atcaagatgc cggcagtgac cagaccggcg ctcgtgccgg tcgacggcag gctgcctgcc
     7381 tgacctccgg gcgtggctgt gatccctgcc atcgccgtag gcgggggcgt ggccaaagcc
     7441 gtggcggtcg caagctcaaa gcaggcgcca gactgcggaa gcaactgatc gttgctgccc
     7501 catcccacga cgccggtccg cgtcgccact ctccaccacc acacgccgtc ggcgcgctcc
     7561 ggcccggccg tcacaagaac gacgtcgcct cgccccacga tctgcacgtg gttggcgccg
     7621 aaacccggcg cgtcgcccaa aatcagtccc tgcgctccca cgacgcgcgc acaggcattc
     7681 actcgaatgc cgaggctggg cgtagccggc ggacctggcg ggcggggcgc gccacgggtt
     7741 gttgtcggct gaggcggcac gcccgtggca acgaccgtcg atgtactcgt tggcgccgcc
     7801 gaagtgctcg tcggcggcac caaagtgctc gtcggccgca ccggagtact ggtcggcgtg
     7861 ctgatcacga cctgcgtcgg cgtgggcgac ggtggcacct gctccagcct ggagtgcgga
     7921 gacggcctga atccgagact gctcaccgcc accagtagcg caaaggcggc tgcgacgccg
     7981 agcgccaagc gatcgcgcat tcacctctcc ggtttgccag ctcggcgcga tcgccgacct
     8041 ggcggcgcaa agtgacgtaa ttctagcaga aatcaaagct agaagcaatt agaacaggac
     8101 agaagtgcta tcaggccggt gtgcgcaagc gcgcgggcca gtattcagag gggctccgct
     8161 acggcagggg aaggatcagg ctgaccttcg tgccgcgtcc cggcgtgctg tcgatctgga
     8221 cctggccgcc catcatctcg acgcgctcgc gcagcgtgct caagccgatg cctttgcgag
     8281 cggacagcgt ctcgaccacg tcgaagccac tgccgttatc ctcgacggag acgcgcacgg
     8341 catcctcggt cagatcgaga aacacctgtg cccgggtggc gtgcgcgtgc tggcgcacgt
     8401 tgcccagcag gtcctggacg atacgaaaca gcgccatctc tttgtagccc gtgaatcggc
     8461 gctcgttgcc cgtcacggtc agcgcgatgg gcatgccgct cttctccgcg cagccgtcca
     8521 cgtagcgctt gagcgtgggc accaggccga gatcgtccag catcatcggc cgcaggtcga
     8581 acatgaagtc gcggactttc tggaacgtgc tcgtgacggc ggttttgaga ttgctcaact
     8641 cggagcgcgc gcgcttggca tcgtggtcga agagccgctc gcaaatctcg gcctgcaaga
     8701 tcagattggt gagcgattgg gccgggccgt cgtgcaactg cctgacgagg cgctgtcgct
     8761 cctgctcttg cgtttcgatg atgcgcacga tcgccggctg gcccgcatcg gcggcgccag
     8821 aatcgatccc cacgccgggt gccacgacgc cagcgccgtc ggagacggcg ctgacagagc
     8881 gattcagggc attggccaga cggctcagat tctgctgatc gctctggagc ttctccaact
     8941 gactcttcat catgaccagg cgcttctgcg cctcctgcgc ctcggcgtaa accgcctgca
     9001 gatcgctgcg gggcacggta tcgatgtgga tctctgtctg cttcacccgg ttggcagcct
     9061 gcgcgttgcg ctgcgccaga cgatccacct cgccgcccgt ctgctggatc agcaggtcga
     9121 tctccttcag ttcccgctgc gtttgctcgt actcggcctt cagctcgtcg aggagatgag
     9181 aggaagaccc cgagcctgcc gcccggccgt gcagtgcgtc catgattgat gccgcctccc
     9241 gtgcagcgcg cggcgcgcct atggatcctt agacagggac ggccgcagcc caagccggtc
     9301 gccttgaccg acctgagtat cctgcaggcg cacccatccc cggcgcaggg cgtacacggc
     9361 tgcctgcgtg cgatcctcga cgttcagctt gcgcaggatc gaggtcatgt ggttcttcac
     9421 cgtctggtgg ctgatgccca actgatgggc gatctccttg ttgctcaggc cgcgtgtgat
     9481 gtgctgcagg atctccattt cgcggggcga gagagggacg aacgcctcgt cgcccaactc
     9541 gccttcgacg atgccgaacc gctcgctttg cttgtacagc cacggcgcga gctgagcgtc
     9601 gtccatgacc aggccgctga cgatgtagcg gcccacattc acctcgcgca ccgcctcgac
     9661 cagcttgtgg ggctcgacgt ctttggggaa gtagccgtgc gcgccggcct tgatggcgtg
     9721 gaacatctgc tcctcgtcgt cgtagccggt gaggatcacg gcgcgcgcgc cgggcgcgtc
     9781 ctggcgcagc cggcgcgcca cctgcaggcc gttgagcgtc gggatattga cgtccacgag
     9841 cacgacgcgc ggcgacagct cgcgcgccgc gcgcagcgcc tcttcgccgt cggacgcttc
     9901 gccgacgacg accaggtcag gctcggcgct caacacctcg cgcaggccct ggcggaatag
     9961 agggtgatcg tctacgatga gaaccgtgat cttctgctgc atggcaaccc cgttccagta
    10021 taccaaaaag gctagtcagg cgcaagcaga tggtatgcca ggcgcgttgc agtttcgtag
    10081 caaaccggct cttcgttccg cgtcgtgctc ccgctcacgg cctgccccac ctcgcccgaa
    10141 agatcagcca gtgctttggc gcgaagggcg aatctccctc gaacgccggt tccagttcgg
    10201 gcgcgtcgag cagcgctgcc gaatagaaat tcggcggcgc ggcgacgaaa tccgctgcgc
    10261 gcgaccgcag cagcgccagc agcgccgcct cgtcgcgaat gatcgggatc acttcgggtg
    10321 agaccaaccc cgccaggtcg agcagcggcc ggcggctgaa atagccgatc gcgccgatgt
    10381 cgtgcgcggc gaccagcgct cccggcggcg tgttcgattc gatccagcgc gccgacgcga
    10441 ccagctcgtt ctcgatcacg ttcacgtcga cgcgatatgc gccggcgccg acgagccaaa
    10501 atgcagccgt cgcggcgatc accgatccca cccacaccgg cctggcgatc cggtggcgtt
    10561 cccgactcag tcgatccaat tttgctcggc ctggattgcc aaatccaggc ccaaaccggg
    10621 gactggacaa tcccctcaga gcattttcgg atcaaccgag actggatggc gcctgcccat
    10681 aacaaaggga cgcaaagcac ggctgtgctg tgcgtccccc gcctgtgacc tgagagagtc
    10741 gtccccatgc cgcatggagg agtttgccct tcggtgtcgc cggccacacg gcgaagcgac
    10801 tcggcgactt tccagaggat gagcaggctt taccccggct agattgtagc cgcgtgcctc
    10861 aggcgtgtca accgcaagat gtcacgattc gcgcctgtcc tttgtcggag agcg
//
LOCUS       DBNHXB010000112         5553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_356873, whole genome shotgun sequence.
ACCESSION   DBNHXB010000112 DBNHXB010000000
VERSION     DBNHXB010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_356873"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="AC162G_04360"
     CDS             complement(<1..839)
                     /locus_tag="AC162G_04360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR5075079.1"
                     /translation="MNISIIGTGAMASLFGARLSPVAAVTLMGTWEAAVKAIRRNGIL
                     VEGEGTFRVHAVPDPDDAPPADLAIVLVKTYQTERAARWAARTLKPPGVALTIQNGLG
                     NVETLAAQVGVDRAMLGVTTQGATLLKPGQVRSGGRGTTHLGFLPIERAAPRDWSADS
                     LAYEVTALLNAAGLASIVSEDVEALAWGKVIINAAINPLTAMLRVPNGALVESADTLE
                     LMRSIAGEAASVARAYRIALPYADPFERARQVAQATATNRSSMLQDVLKGRPTEIDAI
                     NGK"
     gene            complement(836..1726)
                     /gene="panB"
                     /locus_tag="AC162G_04365"
     CDS             complement(836..1726)
                     /gene="panB"
                     /locus_tag="AC162G_04365"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018035492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR5075080.1"
                     /translation="MERKKVTIPTLHGKKGRGEPITMVTAYDYPGGLAADAAGIDMIL
                     VGDSLGMVVLGYDSTVPVTMDEMISHAAAVKRGAKFSFIVGDMPFLSYQTDAKEAIRN
                     AGRFLKEASSDAVKLEGGAEMAETVKAIVDAGIPVMGHIGLTPQSASKLGGYRVQGKT
                     ARAALKLLDDALALEQAGAFSVVLETVPDRVAELVSKRLSIPTIGIGAGVGCDGQVLV
                     FHDLLGYFDRFSPRHNKRYANIRETIVNALSEYRREVEGRAFPTAENSFAIGDDEFQA
                     LTHALEEAGERVSGAEGVQA"
     gene            complement(2035..3282)
                     /locus_tag="AC162G_04370"
     CDS             complement(2035..3282)
                     /locus_tag="AC162G_04370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5075081.1"
                     /translation="MEPHDGILIIGGGVIGVCSAYYLAEKGQRVTLVEQGDIAAGSSY
                     GNAGLIVPSHSVPLAAPGVLAQGLRWLLDPESPFYIKPRFDFALFGWLLRFAAACREG
                     PMRRAIPVLRDLGQASLALFDELAALDGLEFGYERRGSLLIYRTRRGYDGGVEEAHLL
                     NEFDVAAQPLDATQVRQLEPNVLPGIVGGVYFSTDTHLRPADFVQRLAARAEKLGACI
                     RTKTEVIGFETSGRKVVRVKTTRGDFVPAQVVLATGAWSPVVARDLRLRVPIQAAKGY
                     SITIKRPDKSPTIPLLLGEARVAVTPMGDVLRLAGTLELAGLDLSIHSRRVTAIRRAA
                     KSYLPGLEKIEPLEIWRGLRPCTPDGLPIVGRARGFDNLIVAAGHAMVGMTLGPITGK
                     LVAQLLCGEKTDVDVRALRLERF"
     gene            3554..5023
                     /locus_tag="AC162G_04375"
     CDS             3554..5023
                     /locus_tag="AC162G_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HIR5075082.1"
                     /translation="MGQSAVDYSRKWHVMVAVTMGIFLATIDGSIVNLALPTLVRELR
                     ADFAAVQWVVLAYLLTLATLLLSVGRLADMLGKKTIYTLGFVIFTAGSVLCGLSPSIY
                     WLIGFRVLQAVGAAMILALGAAIVTEAFPPSERGKALGLIGSAVSIGIVVGPTLGGVL
                     IDAFSWHAIFFVNLPVGIAGTLLAMRFVPDIRPVSRQRFDIPGALLLCAGLLCLLFAL
                     TLGQQLGFGDSRIMMLFAGFAAFIAAFIAVELRVRQPMIDLRLFRNRLFSVNLLTGFL
                     TFVALAGSLFLMPFYLQNVLGYDTRQAGLLMASVPIGLGVTSPISGSLSDRFGTRRIA
                     VIGLAALLLTYVLLSTLSAETDAIGFILRFAPLGIGMGIFQSPNNSAVMGTAPRERLG
                     VVSGLLAITRTVGQTTGIAILGAVWAGRVLVHMGGPLPGGATSAPIEAQVAALHEAYL
                     VMAALVAAALGFGLWALAQERRAKVAAALAVEPSTQEGA"
     gene            complement(5095..>5553)
                     /locus_tag="AC162G_04380"
     CDS             complement(5095..>5553)
                     /locus_tag="AC162G_04380"
                     /EC_number="6.3.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR5075083.1"
                     /translation="LEVGERAAGHTPGVKKVGGILTETAIAGERLDYAVVGIGLNVNV
                     DFAGQPELAETATSIMLKLGREIDRLSVLAAVVDRFAERYEWLDEGDALRAAWSERLV
                     TLQRHVEARVGDSVLTGWAEAVDDDGALLLRAGDGSMHRLLAADVTLREP"
BASE COUNT          880 a         1857 c         1780 g         1036 t
ORIGIN      
        1 attttaccat tgatcgcgtc gatctctgtc ggccggccct tgagcacgtc ctgcagcatc
       61 gacgagcggt tcgtcgccgt cgcctgcgcc acctgccggg cgcgctcgaa cggatcggcg
      121 tagggcagcg cgatgcgata cgctcgggcg accgacgccg cttcgccggc gatcgagcgc
      181 atcaactcga gcgtgtcggc ggactcgacg agcgcgccgt tcggcacgcg cagcatggcc
      241 gtgagcggat tgatcgccgc gttgatgatg accttgcccc aggccagcgc ctcgacgtcc
      301 tcgctaacaa tgctggccag gcccgccgcg ttcaggagcg cggtgacctc gtaggccagg
      361 ctgtcggccg accagtcgcg cggcgcggcg cgctcgatcg gtaagaaacc caggtgcgtc
      421 gtgccgcgcc cgcccgatcg cacctgaccc ggtttgagga gcgtcgcacc ctgcgtcgtc
      481 acgccgagca tcgcccgatc gacgcctacc tgcgccgcca acgtctcgac gttgcccagt
      541 ccattctgaa tcgtcagcgc gacgcctggc ggcttcagcg tgcgcgcggc ccagcgcgcc
      601 gcccgctcgg tctggtaagt cttgaccaaa acgatggcga gatccgcagg cggggcgtcg
      661 tcgggatcgg gcacggcgtg aacgcgaaac gtgccctccc cttcgacgag gatgccgttg
      721 cgccggattg ccttgacggc ggcctcccac gtgcccatca gcgtcacggc cgcgactggc
      781 gacaggcgcg cgccgaacaa cgaggccatc gcccccgtgc cgatgatcga gatgttcatg
      841 cctgcacccc ctctgcgccg ctcacgcgct cccccgcttc ttcaagcgca tgcgtcagcg
      901 cctggaattc gtcgtcgccg atggcgaacg agttctccgc cgtgggaaat gcgcgcccct
      961 cgacttcgcg ccggtattcg ctcagcgcgt tgacgatcgt ctcgcggatg ttggcgtagc
     1021 gcttgttgtg ccggggggag aagcgatcga agtagccgag caggtcgtgg aagacgagca
     1081 cctgcccgtc gcagccaacg cccgcgccga tgccgatcgt cgggatgctc aagcgcttgc
     1141 tgaccagctc cgcaacgcgg tcgggcacgg tctcgagcac gacgctgaac gcgccggcct
     1201 gctcgagcgc gagcgcgtcg tcgagcagct tgagcgcggc tctcgccgtc ttgccctgca
     1261 cgcgataacc gcccagtttc gacgcggatt gcggcgtcag cccgatgtgc cccatcacgg
     1321 ggatgcccgc gtcgacgatg gccttgacgg tctcggccat ttccgcgccg ccctcgagct
     1381 tcaccgcgtc actgctagcc tccttgagga agcggcccgc gttgcggatg gcttctttcg
     1441 cgtcggtctg gtacgagagg aagggcatgt cgccgacgat gaacgagaac ttcgccccgc
     1501 gcttgacggc ggcggcgtgc gagatcatct cgtccatcgt cacgggcacg gtcgagtcgt
     1561 aaccgagcac gaccatgccc agcgaatcgc cgacgaggat catgtcgatc ccggcggcgt
     1621 cggcggccaa cccgccgggg tagtcgtacg cggtgaccat cgtgatcggc tcgccgcgtc
     1681 ccttcttgcc gtgcagagtg gggatggtga ctttcttgcg ttccatgttg actctccttc
     1741 aggttgggct gatagcaggc cgtgaaacgt gatgcgtgaa acgtaaagcg cctgttgcga
     1801 ggatcgcaag caggcgcgag ggagcacgat gctcttgtgc catctccgtc tcggtcgcgg
     1861 tacgatccaa gcggctggtt aatcggttcg tcgaatagtc gctcgctgcc ctcttcggct
     1921 gctcagagtg ggtcagcggc ggttagatta tatcgcgctt ttcgagatgc ggctagtcgc
     1981 gaagggtatg ccgcaaaagt gtcggtgacg ggcaatgacc tgccgccgtc accttcaaaa
     2041 ccgctccaat ctcaacgcgc gtacatccac gtcggtcttc tcgccgcata gcaattgcgc
     2101 taccagcttg ccggtgatcg ggccgagcgt catgccgacc atcgcgtgac cggcggcgac
     2161 gatgagattg tcgaatccgc gcgcccggcc gacgatcggc agcccatccg gcgtgcaggg
     2221 tcgcaggccg cgccagattt cgagcggctc gatcttctcc aatcctggca ggtagctctt
     2281 ggccgcgcgc cgaatcgccg tcactctccg ggaatggatc gacaggtcaa gccccgcgag
     2341 ttcgagcgtt cccgccaggc gcaagacgtc gcccatcggc gtgacggcga cgcgggcctc
     2401 gcccagaagc aaagggatcg tcgggctttt atctggacgt ttgatcgtta tgctgtatcc
     2461 cttcgccgcc tgtataggca cacgcagccg aaggtctcgt gcgacgacgg gcgaccatgc
     2521 ccctgtggcc aggacgactt gagcaggaac gaaatcccct cgcgtcgtct taacgcgcac
     2581 cactttccgc ccggacgtct cgaacccgat cacttcagtc ttcgtccgga tgcaggcccc
     2641 cagcttttcg gcgcgcgccg cgagtctctg cacaaagtca gccgggcgaa ggtgagtgtc
     2701 ggtcgagaag tacacgccgc cgacgatgcc gggcaggaca ttcggctcga gctggcgcac
     2761 ctgcgtggcg tcgagcggtt gagcggcgac gtcgaattca ttcagcagat gcgcttcttc
     2821 gacgccgccg tcgtagccgc gccgcgtcct gtagatcagc agcgagcctc tgcgctcgta
     2881 gccgaactcc aggccgtcga gcgccgccaa ctcatcgaac agcgcgagac tggcctggcc
     2941 caggtcgcga agcacgggga tcgcccgcct catcgggcct tcgcggcagg ccgcggcgaa
     3001 cctcaacagc cacccgaaca gcgcgaagtc gaagcgcggc ttgatgtaga agggactctc
     3061 gggatcgagt agccagcgca atccttgcgc cagaactccc ggcgcggcga gcgggacgct
     3121 gtgacttggc acgatcaacc cggcgttgcc gtaggagctg cctgcggcga tgtcaccctg
     3181 ctcgacgagc gtgaccctct gtcccttctc ggccagatag tatgccgagc agacgccgat
     3241 gaccccgccg ccgatgatga ggatgccgtc gtgaggttcc attctctttt tagagtctat
     3301 gccaacccgt gtcccaggtc aagtgaagcg tgttactcgg tgcatttcaa ttttgtggca
     3361 gacctaaccc cctcggcccc ctttcctaca agggaagggg gagcagcgcc gctccccttc
     3421 ccgtgttcgg gagggattgg gggaggggaa gcgccgcatg cccaggacgc aaacgacaaa
     3481 tcaggataaa atatccccct ggcatccgcg ccgggggttg gagatttaca cctgggtgct
     3541 cgaaggatag tccatgggac aatctgcagt cgattacagc cgcaagtggc acgtcatggt
     3601 tgccgtcacg atgggcatct tcctggcgac gatcgacggc agcatcgtca acctcgcgct
     3661 tccgacgctc gtgcgcgagc tgcgagccga cttcgcagcc gtccagtggg tcgtcctggc
     3721 gtacctcctc acgctcgcca cgctgctgtt gagcgtcggc cggctggccg acatgctggg
     3781 caagaagacg atctacacgt tgggattcgt gatcttcaca gccggctcgg tgctgtgcgg
     3841 gctgtcgccg tcgatctact ggctgatcgg cttccgcgtg ctgcaggctg tgggcgcggc
     3901 gatgatcctg gcgctgggcg cggcgatcgt caccgaagcc ttcccgccga gcgagcgcgg
     3961 aaaggcgctg ggactgatcg gcagcgccgt ctcgatcggg atcgtcgtcg gcccgacgct
     4021 gggcggcgta ttgatcgacg cgttctcgtg gcacgccatc ttcttcgtca acctgccggt
     4081 cggcatcgcc ggcacgctgc tggcgatgcg cttcgtgccc gacatccggc cggtcagcag
     4141 gcagcgattc gatatccctg gcgcgctcct gctgtgcgcg ggcctgttgt gcctgctgtt
     4201 tgcgttgacg ctcggccagc agctcggatt cggcgacagc cgtatcatga tgctcttcgc
     4261 cggattcgcc gcattcatcg ctgcattcat cgccgtcgag ctgcgcgtca ggcagccgat
     4321 gatcgatctg cggctgttcc ggaatcgact gttcagcgtc aatctcctga ccggcttcct
     4381 gaccttcgtc gcgctggccg gctcgctgtt cttgatgccc ttttatctgc agaacgtgct
     4441 cggctacgac acgcgccagg ccgggctgct gatggcgagc gtgccgatcg gcctgggcgt
     4501 cacctcgccg atctccggct cgctgtcgga tcgattcggc acgcggcgca tcgccgtgat
     4561 cgggctggcg gcgctgctgc tgacgtatgt gctgttaagc acgctgagcg ccgagacgga
     4621 tgccattggc ttcatcctgc gcttcgcgcc gctcggcatc ggtatgggca tcttccagtc
     4681 gccgaacaat agcgccgtca tgggcacagc gccccgcgaa cggctgggtg tcgtgtccgg
     4741 cctgctcgcg atcacgcgca ccgtgggaca gaccaccggc atcgcaatcc tcggcgcggt
     4801 gtgggctggc cgcgtcctgg tccacatggg cgggccgctt ccgggtgggg cgacgtccgc
     4861 gccgattgaa gcgcaggtcg cggcgctgca cgaggcatac ctggtgatgg cagcccttgt
     4921 cgcagccgcg ctgggattcg gcctatgggc gttagcgcag gagcgccgcg ccaaagttgc
     4981 cgccgcgctc gccgtcgagc cgtcgacgca ggaaggggca taagggcatg tgcgacgcac
     5041 tttagaagtg cgtggcacat aggtatttgc ggttcgcagt ggcgactttc gtcgctatgg
     5101 ctctcgaagc gtcacgtcgg cggccagcaa acgatgcatg ctcccatcgc cggcgcgcaa
     5161 gagtaacgct ccatcgtcgt cgaccgcttc ggcccagccg gtgagcacgg aatctcctac
     5221 cctggcctcg acgtggcgct gcagtgtgac cagccgctcc gaccaggcgg cgcgcagtgc
     5281 gtcgccctcg tctaaccact cgtaacgttc tgcgaatcga tccacgaccg ccgccagcac
     5341 gctcagccga tcgatttcgc gcccgagctt gagcatgata ctggtggccg tctcggcaag
     5401 ctccggctgg ccggcgaaat cgacgttaac attcagccca atgccgacga cggcataatc
     5461 cagccgctcg cctgcgatgg ccgtctccgt gagaattccc cccacttttt ttacacccgg
     5521 cgtgtgaccg gccgcacgct ctccaacctc caa
//
LOCUS       DBNHXB010000113         5528 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1531542, whole genome shotgun sequence.
ACCESSION   DBNHXB010000113 DBNHXB010000000
VERSION     DBNHXB010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1531542"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="AC162G_04385"
     CDS             <1..1200
                     /locus_tag="AC162G_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5075084.1"
                     /translation="AASVSATAARFQISIDPAVQVRYLSVGEQQRVEILKALHRECRV
                     LILDEPTAVLTPQEAEALFAAIRRLVEQGLSVVFISHKLDEVFAVSDRVTVLRDGRVV
                     GETPTRDAGHASLARLMVGREMAGVSREGVAAAGAPLLRLVDVHALDDRRLPALRGLT
                     LAVGAGEIVGVAGVAGNGQRELAEVLCGLRHPSQGRVFVGNDPVDVTRATPAEVAARG
                     VGRIPEDRLSAVVADLTVADNLALEHLGEFTRRISPLPSWVRTGEGLGVRVLDRDRMR
                     QAAEALISEYGIKAAPGDRARTLSGGNMQKLILARALSRNPRVVIAAEPTRGLDVGAT
                     EYVRGKLLEARDRGSAVLLISEDLDEVLALSDRIAVIYEGRLTGVLSASEADPERLGL
                     MMAGQGR"
     gene            1277..2380
                     /locus_tag="AC162G_04390"
     CDS             1277..2380
                     /locus_tag="AC162G_04390"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5075085.1"
                     /translation="MLKFRLEAGGAPAWQRGLIPILAVLVTFVLTAVLVLLAQANPLE
                     AFYYFLVEPFTARSTAIEILVKSTPLLLTGVAVAFAFVAGYFNIGVEGQLYAGAIAAA
                     WLGPQLAGVPAIVAVPLMLIAGFAGGMLWALPPAWLKARLNVDEVVTTLLLNSVIVFF
                     AGALLNGPWRDPISQWPQSPEIAASAHFPVLLARSRLHLGALAGVVAAAVFGWVLWRT
                     ALGLRMRAVGLGRDAARFMGIDVARTTLIAALVSGGIAGIAGVGEVAGIHYHLIESIS
                     PGYGYSGIVVATLGGLNPIGVGLSAVFIGLVLTGAQEVSRALGVPSYLGDVVQATLLL
                     VVLALLVLSRYRLKISRTQMQSAQIHADKVESA"
     gene            2425..3333
                     /locus_tag="AC162G_04395"
     CDS             2425..3333
                     /locus_tag="AC162G_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5075086.1"
                     /translation="MDVTIDLAAATLRVATPILFAALGELYCERAGILNLGIEGTMFI
                     GALAGFLVSYVSQSVWLGLLAAIVAGLLFGALMALLTVRLGLNQHVSGLGTTLLASGL
                     ALFIYRLTFGAPSVQPKSPAFPLIEPPAIFGPLAPLFKQYGLTYVALALVPLTWWILR
                     RTTFGLKLRAVGENPEAADTAGVNVFRVRYAALMIGGALMAVGGAFLSLAQLGTFTFG
                     IIAGRGWVAIALVIFGNWQAFRVFGAALLFGALSVLQLRLQAEGVDIPYQALLALPYL
                     VTILALAVAGRNASYPAALLKPYRRE"
     gene            complement(3349..4155)
                     /locus_tag="AC162G_04400"
     CDS             complement(3349..4155)
                     /locus_tag="AC162G_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HIR5075087.1"
                     /translation="MSSPFPGMDPYLEEPARWPDVHQRLITYIADALQPQVRPRYYAR
                     IGERVYILNSPQALYPDIMLIQRPLREPAAAAATATAKAEAEAALDTPTILTLPPVEH
                     REPFVEIVHAGGDEVVAVIEVLSPTNKAPGEGHQLYRRKQQHILDSPAHLIEIDLLSA
                     GLLTVAISEEGLASLPPHRYLVSVQRAPDRYRFEVYAIPLQQRLPRMHIPLRGPDPDV
                     GLDLQAVFTQCYDNGGYADLIDYRRPPSASLSQEEAAWLDGLLKGKGLRA"
     gene            4367..>5528
                     /gene="ettA"
                     /locus_tag="AC162G_04405"
     CDS             4367..>5528
                     /gene="ettA"
                     /locus_tag="AC162G_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HIR5075088.1"
                     /translation="MVRVGKIYPPNKQVLRDISLGFFYGAKIGVLGLNGAGKSTLLRI
                     MAGVDQDYVGETMPTKGYTTGMLEQEPQLDPDKTVRQVVEQAVRPIVDLLAKYDEINN
                     RFAGDMTPEEMEKLIEEQGRVQEELDKRDAWNLDSRLELAMDALRCPPGDTPISTLSG
                     GERRRVALCRLLLTEPDILLLDEPTNHLDAESVAWLERHLQQYKGTVIAVTHDRYFLD
                     NVAGWILELDRGYGIPWKGNYSSWLEQKQQRLALEEKADVKRIKTLQRELEWIHASPV
                     ARQAKGKARITAYNSLLSQEVEKRREELEIYIPPGPRLGDIVVEVKGVSKTYGDNALF
                     ENLTFELPPGGIVGVIGPNGAGKTTLLRMIIGAEEPDSGTFKIGETVALGTID"
BASE COUNT          884 a         1688 c         1944 g         1012 t
ORIGIN      
        1 gcagcttcgg tgagcgccac ggccgcgcgc tttcaaataa gcatcgatcc ggcggtgcag
       61 gtgcgctatc tgtcggtggg cgagcagcag cgcgtcgaga ttctcaaagc cctgcaccgc
      121 gaatgccggg tattgatcct cgacgagccg acagcggtgt tgacgccgca ggaagccgag
      181 gctctattcg cggcgatccg gcggctggtg gagcagggcc tgtcggtcgt gttcatcagc
      241 cacaagctgg acgaggtgtt cgccgtcagc gaccgggtca cggtcttgcg tgatgggcga
      301 gtcgtgggtg agacgcccac gcgcgacgcc ggccacgcga gcctggcgcg gctgatggtc
      361 gggcgcgaga tggccggcgt ctcgcgcgag ggcgtggccg cggccggcgc gccgctgctg
      421 cgcctggtgg atgtgcacgc actggacgat cgccgcctgc cggcgctgcg cggcctgacg
      481 ctggcggtgg gtgcgggcga gatcgtcggc gtggccggcg tcgcgggcaa cggccagcgc
      541 gagctggccg aagtgctctg cggcttgcgc catcccagcc agggccgcgt cttcgtcgga
      601 aacgatcccg tcgacgtcac gcgggcaacg ccagccgagg tggccgcgcg cggagtgggg
      661 cgcattcccg aggatcggtt gtcggccgtc gtggccgacc tcacagtcgc agataatctc
      721 gcgctcgagc acctgggcga gtttacacgc cgaatctccc ctctcccgtc gtgggttcgg
      781 acgggagagg ggttgggggt gagggtgctc gaccgcgata gaatgcgcca ggcggccgag
      841 gcgctgatct ccgaatacgg catcaaggct gcgccgggcg accgggcgcg cacgctgtcc
      901 ggcgggaata tgcagaagct gatcctggcc cgcgccctct cgcgaaaccc gcgcgtcgtg
      961 atcgcagcgg agccgacgcg cgggctggac gtaggcgcga ccgagtacgt gcgcggcaag
     1021 ctgttggaag cacgcgatcg cggctcagcg gtgctgctta tctccgagga cctcgacgaa
     1081 gtgctggcgc tgtcagatcg catcgccgtc atctacgaag ggcggctgac tggcgtgttg
     1141 agcgcgagcg aagcggaccc ggagcggctg gggctgatga tggcggggca gggtcggtag
     1201 acagttggca gtcatcagtt atcagtaatt agttatcggt aattggtgga tcgcggttgt
     1261 agagtaacga ggcggcatgc tgaagtttag actcgaagcg gggggagcgc ccgcgtggca
     1321 gcgcgggctg atcccgatcc tggcggtgct cgtcacgttc gttctcaccg ccgtgctcgt
     1381 gctgctggcg caggctaacc cgctcgaggc cttttactac ttcctggtcg agccattcac
     1441 tgcccgctcg acggcgatcg aaatcctggt gaagtcgacg ccgctgttgc tcaccggcgt
     1501 ggcggttgcg ttcgccttcg tcgccggcta tttcaatatc ggcgtggagg gacaactgta
     1561 cgccggtgcg atcgcggccg cgtggctcgg cccgcagttg gccggcgtgc ccgcaatcgt
     1621 cgccgtcccg ctcatgttga tcgccggctt cgccggtggc atgctgtggg cgctgccgcc
     1681 ggcctggctg aaggcgcgcc tgaacgtcga cgaagtcgtg acgacgctgc tgctcaactc
     1741 ggtgatcgtg ttcttcgccg gcgcgctgct caacgggccg tggcgcgacc cgatcagcca
     1801 gtggccgcag tcgccggaga tcgcggcgag cgcgcatttc cccgtgctcc tcgctcgctc
     1861 gcggctgcac ctgggcgcgc tcgccggcgt cgtggcggct gccgtgttcg gttgggtgct
     1921 gtggcgcacc gcgctgggcc tccgcatgcg cgccgtgggg ttgggtcgcg acgccgcgcg
     1981 tttcatgggt atcgacgtcg ctcgcacgac gctgatcgcc gcgctcgtca gcggcggcat
     2041 tgccggcatc gcaggcgtgg gcgaagtcgc cggtattcat tatcatttga tcgagagcat
     2101 ctcgcccggc tacggctact cgggcatcgt cgtggcgacg cttggcggct tgaatccgat
     2161 cggggtgggg ctgtccgctg tcttcatcgg cctggttctc accggcgccc aggaggtgag
     2221 ccgcgcgctg ggcgtgccga gctacctggg cgacgtcgtg caggcgacgc tcttgctggt
     2281 ggtgctggcg ttgctggtgc taagccggta ccggctgaag atttctcgga cacagatgca
     2341 aagcgcgcag atacacgcgg ataaggtcga atcggcgtaa tcaatttccc attacgtttt
     2401 gttaaaaccg aaggctacgg catcatggac gtaacgatcg atctggcggc ggcaacttta
     2461 cgcgtggcga cgccgatcct gttcgcggcg ctgggcgagc tatactgcga gcgggcaggc
     2521 atcctgaatc tcggcatcga gggaacgatg ttcattggcg cgctcgccgg tttcctcgtc
     2581 tcctacgtga gccaatcggt ctggctgggg cttttggcgg cgatcgtcgc cggcctgctg
     2641 ttcggcgcgc tgatggcgct attgacggtc agactcggcc tcaaccagca cgtatcgggc
     2701 ctgggcacaa cgctgctggc gagtgggctg gcgctgttca tctaccgcct gacgttcggc
     2761 gcgccttcgg tgcagccgaa aagtcccgcg tttcccctga tcgagccgcc tgccattttc
     2821 ggcccgctcg cgcctctgtt caagcagtat ggcctgactt acgtcgcgct ggcgctcgta
     2881 cctttgacgt ggtggatact gcgccggacg acgttcggcc tgaagctgcg cgccgtcggt
     2941 gagaaccctg aggcagccga cacggccggc gtgaacgtgt tccgcgtgcg ctatgcggcg
     3001 ctgatgatcg gcggtgcgct gatggccgtc ggcggcgcgt tcttatcact cgcgcagctc
     3061 ggcacgttca ccttcggcat catcgccggg cgcggctggg tggcgatcgc gctggtgatc
     3121 ttcgggaact ggcaggcgtt cagggtgttc ggcgcagcgc tgctgttcgg cgcgctctcg
     3181 gtgctgcagt tgcggcttca ggcggaggga gttgacatcc cgtaccaggc gctgctggcg
     3241 ctgccttacc tcgtcacgat cctggcgctg gccgtggccg ggcggaatgc gtcgtatccg
     3301 gcggcgctgt tgaaaccgta tcgaagagaa tgagagatgc ttcaccgttc aagcgcgcag
     3361 acctttacct ttcagcaggc cgtccagcca ggcggcttcc tcctgtgaaa gcgatgccga
     3421 tggcgggcgc cgatagtcga tcaaatcggc gtatccgccg ttgtcgtagc attgcgtgaa
     3481 gaccgcttgt aagtccaggc ccacgtcggg atccggccca cgcagcggga tgtgcatgcg
     3541 gggcaggcgc tgctggagcg ggatcgcgta cacctcgaag cgataccggt cgggcgcgcg
     3601 ctgcacgctg accaggtatc gatgtggcgg cagcgaggcg agtccctcct ccgagatggc
     3661 caccgtgagc aagcccgccg acagcagatc gatttcgatc agatgagccg ggctgtccag
     3721 gatgtgttgc tgcttgcggc gatagagctg atgcccttct ccgggcgctt tgttggtggg
     3781 actcagcact tcgatgacgg ccacgacttc gtcgccaccg gcatggacga tctccacgaa
     3841 aggctcgcga tgctcaacgg ggggcagggt gaggattgtc ggcgtgtcca acgccgcttc
     3901 agcctcggct ttcgccgtcg ccgtggcggc tgccgcggcc ggctcgcgga gcgggcgctg
     3961 gatcagcatg atgtccggat acagcgcctg gggtgagttc aagatgtaca cgcgctcgcc
     4021 gatgcgggca tagtagcgcg ggcgcacttg cggctggagc gcgtcggcaa tatatgtgat
     4081 cagcctctga tgcacatccg gccaacgcgc cggctcttcg agatagggat ccattccggg
     4141 aaatgggctg gacatcgccg tgctcctccc aatacgagta tagcataatt ccactgcggc
     4201 agtcatcagc cggttcatga gaacgtatcg ggacacgatg cggctggaca tgcctcgcaa
     4261 cggccccgct cttctccgat catcagctat aatcaaccct gaattcgtca caagttctcc
     4321 gcagtcagcg attgggggtc gcgtgagcga gcaaatcatt tactcgatgg tgcgcgtcgg
     4381 caagatctat ccgccgaaca aacaggtgct gcgcgacatc tcgctcggct tcttctacgg
     4441 cgcgaagatc ggcgtgctcg gcctgaacgg cgcgggcaag agcacgctgc tgcgcatcat
     4501 ggcgggcgtc gatcaggact acgtcggcga gacgatgccg accaaaggct acactacggg
     4561 catgctggag caggagccac agctcgatcc ggacaagacg gtcaggcagg tggtcgagca
     4621 ggccgtgcgg ccgatcgtcg atctgctggc gaagtacgac gagatcaaca atcgcttcgc
     4681 cggggacatg acgccggagg agatggagaa gctgatcgaa gagcaggggc gcgtgcagga
     4741 ggagctggat aagcgcgacg cgtggaatct cgacagccgg ctcgagctgg cgatggacgc
     4801 gctgcgctgc ccgccgggcg acacgccgat ctcgacgctg tcgggcggag agcgccgccg
     4861 cgtcgcgctg tgccgactgc tgctcaccga gccggacatc ctgctcctcg acgagccgac
     4921 gaaccacctc gatgccgagt cggtggcgtg gctcgagcgg cacttgcagc agtacaaggg
     4981 cacggtcatc gccgtgacgc atgaccgcta ctttctcgac aacgtcgccg gctggatcct
     5041 ggagcttgat cgcggctacg gcataccgtg gaagggcaac tactcgtcgt ggctggagca
     5101 gaagcagcag cggctggcgc tcgaagagaa ggcagatgtc aaacgcatca aaacgctgca
     5161 gcgcgagctg gagtggattc acgcctcgcc tgtggcgcgg caggcgaagg gcaaggcgcg
     5221 catcacggcc tacaacagcc tgctcagcca ggaagtcgag aagcgccgcg aggagcttga
     5281 gatctacatc ccgcccgggc cgcgcctcgg cgacatcgtc gtcgaggtga agggcgtgtc
     5341 gaagacgtac ggcgacaatg ccctgttcga gaacctcacg ttcgagttgc cacccggcgg
     5401 catcgtcggc gtgatcggcc cgaacggcgc aggcaagacg acgctgctca ggatgatcat
     5461 cggcgcggag gagcctgatt ccggaacgtt caagattggg gagacggtgg cgctgggcac
     5521 catcgatc
//
LOCUS       DBNHXB010000114         9515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4047710, whole genome shotgun sequence.
ACCESSION   DBNHXB010000114 DBNHXB010000000
VERSION     DBNHXB010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9515
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4047710"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2440
                     /locus_tag="AC162G_04410"
     CDS             <1..2440
                     /locus_tag="AC162G_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5075089.1"
                     /translation="EDAHVIAMPDAGYADFGLAREDVPPGEPIRQTLLGQGSSELQEV
                     VSNMVSAPRAVTTLDIGRVEHYVAYAPLTTAPYSLGLVVPTAELNQPIIAANARVAEE
                     AARNRHFTALALIAVLGLAALASAGIGQLLAAPLERLTRAAHTVTSGDLNAQAEVRSK
                     DEIGALAAAFNLMTARLRDVIGSLEARVAERTQQLRASADVGRAAASILDPDRLMREV
                     VDLIAERFGFYYAAVFILDKSGEHAVLRAATGEAGRALLERGHMLAVGGRSMVGVVTA
                     QRQARIALDVGEEPVRFANPLLPDTRSEIALPLVVGDRVLGALDVQATQEAAFDSASA
                     EVLQSMADQIAVALANATSYAEAQAAARQAHALYVASQQVGRLEVGLKATTDDILRAV
                     SETSGFNHWWVLILDESRKHLNTLSATPDFDRTPSLSVDEHFHSPLVRSALSGETLVL
                     SDPWLDPHRAGDPAEERFPIKSVCTPIVASGRHIGALVVGWPLDGSVIGEPELQVSRS
                     LASLISVAVESRVLFEQTRRALNELDAINRRLTGEAWAEQFQRRADAWRVQVTQSGVK
                     PVEEDRLPEIEMAVTEQQAVAWVHPAGSAGAGEGSKGADQPSPALAQRGSPYRSAMAA
                     PIVLRGEVIGALQVGDVSRARVWRNQDLAFIQAVADQVALAVENARLIEQTQRVARRE
                     KAIAEAADRIHRPVDLDTILRTAIEEVVRITGSTEVSIQLGVGAGTAKSPTLNESTDR
                     NSGSGEFSGLNSRMGETHMVKGEGEWAPSEAGAGVERPRGNGNADEPNLTDSVRPGGS
                     PKPSSEQERDNDDA"
     gene            2430..4634
                     /locus_tag="AC162G_04415"
     CDS             2430..4634
                     /locus_tag="AC162G_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5075090.1"
                     /translation="MMLDRGRRISATAGDRDSDAARRSGQRLPPWTSLPALVAQARVF
                     GTATRRYAAVGVAFGFMFPVVATGIAIGLNNWPLSLESALAVQRAQPLLWIIDTAPFF
                     LGLFAAVAGRHADSLYAANRRLNEMTKELENAKAEAERKVAERTEQVRAGSVVARVAA
                     SILDPDRLLRDVVNLIADHFRFDYAAVFTLDAEGRYAMLREATGDVGQILKQRGYRLE
                     IGGQSMIGTAIAWRKPRLVLDVSREPILRSPPPRGAGTTDLANAAGTTDLANAADRGE
                     GAAEGRFANLLLPNARSELALPLMVGERVLGALDVQSAHEAAFDEASTTVLQSVADQI
                     AIALNNAEQFHRAERQAAALAVLNQLSRDLTMAASLEAIGHAAVTALASLVAVDGLLL
                     GLATPNLETVRIVPLDLQSREQQDSPRSEAEALNAVKGQWESIPPALSAAQTFVGQCI
                     RTGQNVLVADLGALASAPYQDAALARKAGMRSLIGVPLQVGGQTVGALFAGAARLDAY
                     APDDVSRVEQVAAQLAVAVESLNRAEDMRQALEGLDAANRRLTGQAWSAFARRLSRQG
                     VIRIGSHVDDKAPSPEVEEVISTGQLVVHPAKSSTEGAGEGYRVAVPIVLRGVPIGAF
                     RLSVPEHSWSDDLAATLDSIAGHVAQAVENARLVEEAEERAARERALADATERVRSKA
                     DVERILQTAAEELARHLNAAGVAVRFAPGVHGSPDAGQERSIAGNGAEREEA"
     gene            4631..5896
                     /locus_tag="AC162G_04420"
     CDS             4631..5896
                     /locus_tag="AC162G_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017306054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5075091.1"
                     /translation="MTTIPKAQPSLHPTLRPPSPPALPAPMPALSRSPSRSPDPERSE
                     GAGAGEGTAKGQALLKTGFALLASIVTLASCSAPVSPVLTSQPAGGRQPAGEQAAELN
                     IFNWDTYIDPAILDDFEQKYSVKINYDIYASNEDMLATIQAGPTDYDIVVPSDYMVAI
                     MRGQDLLAPLRKDRLPNFEHVDEAFLNPVFDPGNRYCAPYQWGTIGIGYNVRATGREI
                     SSWADLFDPVFAGRVALLDDARGALGVVLLYLGYSPNTTNVVELQTARDFLKQRSRHI
                     AAFVPDTGQDLLVAGDVDLVLEWSGDIFQVMENNPDIRYVIPKEGSIIWSDNMCILSS
                     APHPDLAEKFINYILEPEVGAALSSYVRYASPNRAALPLLPASDRNDPALYPSDEVRQ
                     RLFFLADNGPEAARLFEQAWADILAAHTR"
     gene            5916..8720
                     /locus_tag="AC162G_04425"
     CDS             5916..8720
                     /locus_tag="AC162G_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5075092.1"
                     /translation="MLSEPRVKSARRPAPIRTQLFVGFGLILVLALLIAVIGYVSLRS
                     LQWGTQATIEQAEHIRQLSLAIERDFLSARQSEARFLNSWRSIGPEAAAERYMTEVHD
                     LLAQARAKLDDLDALLLAAGDEQLRSLVEQTAALRPLLDTYETAFHSMATDIQKRADP
                     DIGLDRALKNGLDMLEADVSPLPKTDLLLLVLRLRANEQAYFGAGGQEYYDNVRLAVD
                     KFVNLVKASSLADLSTETTRLVPSDLIDRAQAYFQTFGELVALEQGIQVFTTIFQEVS
                     SDINLITERLNAEGESGLDRARARMQTLGNEITVALAATASLALGLAMVVSWVLARRI
                     VGPLADLSRAALQIGRGNLDVPLPASKIREIADLSETMEQVADQLRALIGSLEARVAA
                     RTEQLRASADVGRAAVSVLDPDRLLYDVVNLITNRFGFYYAAVFLMDEKGRYAVLREA
                     TGQAGRILKERGHKLEVGGRSMVGFVTAQRRPRIALDVGEEAMRFANPLLPDTRSEIA
                     LPLVVGDRVLGALDVQSTREAAFDEASAAVLQGMADQIAIALNNAALYAESQRNVNTM
                     NELLAASRDIARSRTLPELAGLALSRVHALIGVDSYYLGLVNENQTEMRFVVRVLGGV
                     DVSDMVAAHPLGDGLPEHVIRTRKPLRISASEAARAAQSGIPVEEAQRGAFLGVPVVV
                     GERVLGVLGFQDMRPDSFFSDDQERLAVTLARQIAVALDNLRLAEETRRALADLDAAN
                     RLLTGQAWRQYARAARGLSGEWRAGEWLALSEGEGSSRGPQESADGVPGRLTIPLRIR
                     GEAVGEFDLLSLGDGEGWTPEDVAFAQSLVDQVGQTLETVRLLEETERLAGRERLINE
                     INARVRQTVHIDGILQTAVDELGRSLKAARVVARIGVPETSTQETSVHATSGTREPGV
                     RAERGAADPEKRDGRGDDDA"
     gene            8695..>9515
                     /locus_tag="AC162G_04430"
     CDS             8695..>9515
                     /locus_tag="AC162G_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075093.1"
                     /translation="MDEVTMMRSLTSRLRTSLRFMGLPVYPDEERARAARLLLAILSI
                     LLITAVAVLIVSVLDGQSRTAQIVLTGSMILPFAFWLLRRGHLQAASLCVVLVLLSVL
                     TYLLVVGEGIHDIALTAYPTVIVISSLLLGRPGAVIGTVLSIVAVGLVFVAESQGVVT
                     NPFSYTTSPGEFAILVALLCLVSAMVELLASNTRRSLKRARRNEHAQVEANRELQAIR
                     DSLEQRVAERTAQLSVSAEVSRAVTSVLDPDDVIRRVAQLITERFGFYYAAVFLL"
BASE COUNT         1642 a         3048 c         3165 g         1660 t
ORIGIN      
        1 tgaggacgcg cacgtcatcg ccatgcctga tgcgggatac gcggacttcg ggttggcgcg
       61 tgaggacgtg ccgcccggcg agccgattcg gcaaactctc ctcggtcaag gctcatcgga
      121 gctgcaagag gtggtctcca acatggtctc ggcgccgcgc gctgtgacga cgctggatat
      181 cggccgcgta gagcattacg tagcatacgc gccgctgacc accgccccct acagccttgg
      241 cctcgtcgtt cccacagcgg aactgaacca gccgatcatc gcggcgaatg cgcgcgtcgc
      301 cgaggaggcc gcccgcaacc gccacttcac cgcgctcgcc ttgatcgccg ttctcggcct
      361 ggccgccttg gccagcgccg ggatcggcca gttgctcgcc gcgcccctcg aacgcctcac
      421 ccgtgctgcc cacaccgtca cgtcaggcga cctgaatgcg caggcggagg tccgcagcaa
      481 ggacgagatc ggcgcgctgg cggccgcctt caatctcatg accgctcgcc tgcgcgacgt
      541 catcggctcc ctcgaggcgc gcgtcgcaga gcgcacgcag cagttgcgcg ccagcgccga
      601 cgtcggccgc gccgcggcgt ccattctcga tccggatcgc ctcatgcgcg aagtcgtcga
      661 tctcatcgcc gaacgcttcg gattctacta tgccgccgtc ttcatcctgg acaagagcgg
      721 cgagcacgcc gtcctgcgcg cagccaccgg cgaggccggg cgggcgctcc tcgagcgcgg
      781 ccacatgctg gccgtcggcg gacggtcaat ggtcggagtt gtcacggccc agcgccaggc
      841 gcgcatcgct ctcgatgtgg gtgaggaacc ggtgcgcttt gccaatccct tgctgcccga
      901 cacgcgttcc gagatcgccc tgccgctggt cgtcggcgac cgggtcctgg gcgcgctgga
      961 tgtgcaggcc acgcaagaag ctgccttcga ttcagccagc gccgaagtgc tgcagagcat
     1021 ggccgatcag atcgccgtcg cgctcgccaa cgccacttcg tatgccgagg cgcaagccgc
     1081 cgcgcgacaa gctcacgcgc tatacgttgc cagccagcag gtgggtcgcc tggaagttgg
     1141 cctgaaggcg acgaccgacg acatactgcg cgctgtcagt gaaacgtccg gcttcaacca
     1201 ctggtgggtg ctcatcctcg acgagtcgcg caaacacctg aatacattgt ctgccacgcc
     1261 ggacttcgac cgcacgccgt ccctgagtgt ggacgagcat ttccatagcc cgctcgttcg
     1321 cagcgcgctt tccggcgaga cgcttgtcct gagcgacccg tggctggatc cgcatcgtgc
     1381 cggagacccg gccgaggagc gcttcccgat caagtctgtg tgcacgccga tcgtcgccag
     1441 cggccgccat atcggcgcgc tggtcgtcgg ctggccgctc gacgggtccg ttatcggcga
     1501 gcccgagctc caggtaagcc ggtcgctcgc cagcctgatt tcggtcgccg tcgagagccg
     1561 ggtcctgttc gagcagacgc ggcgcgcgct caacgagtta gatgccatca accgccgtct
     1621 gacgggcgag gcatgggctg agcagttcca gcggcgcgcg gatgcctggc gcgtgcaggt
     1681 gacgcagagc ggtgtcaagc cggttgagga ggataggctg cccgagatcg agatggcggt
     1741 cacggagcag caggcggtcg cctgggtaca ccctgcggga agcgcagggg caggcgaagg
     1801 gtcgaagggt gcggaccaac cgtcgcctgc cctcgcgcag cgggggtcgc cctatcgatc
     1861 ggcgatggcg gcgcccatcg tgctgcgcgg tgaggtgatt ggcgcattgc aggtcggcga
     1921 tgtgagccgg gcgcgcgtgt ggcgcaatca ggacctcgcg ttcattcaag ccgtcgccga
     1981 tcaggtggca ctggcggtcg aaaacgcgcg cctgatcgag cagacgcagc gcgtggcacg
     2041 ccgcgagaaa gcgatcgcgg aggcggccga taggattcat cgtccggtcg acctcgacac
     2101 gatcttgcgc acggccatcg aggaagtcgt ccgcattact ggctcgaccg aagtgagcat
     2161 acagcttggc gttggggcgg gcactgcgaa gtccccgacc ctgaacgaaa gcacggatcg
     2221 gaacagcgga tcaggcgaat tcagcggatt gaattcacgg atgggagaga ctcacatggt
     2281 gaagggggag ggggaatggg cgccgagcga agccggggcg ggagtcgaaa ggccgcgtgg
     2341 aaatggaaac gctgacgaac cgaacttgac cgattccgtc cggcccggtg gcagccccaa
     2401 gccttcatcg gagcaggaaa gggacaacga tgatgcttga tcggggcagg cggatatccg
     2461 ccacggcagg tgatcgcgac tccgatgcgg cgcgccgctc cggccagcgc cttccgccat
     2521 ggacgagctt gcctgcactc gtggcgcagg cacgggtgtt cgggaccgcg acccgccgat
     2581 acgcggccgt cggggtcgcc tttggcttca tgtttcctgt cgtcgcgacc ggtatcgcga
     2641 tagggctgaa caactggccg ctttcgctgg aaagcgcgct cgccgtccaa cgcgcgcagc
     2701 ctctgctgtg gatcattgac acagcgcctt tcttcttggg actcttcgcc gccgtggcgg
     2761 gacgtcacgc ggattcgcta tacgcggcca accggcgcct caacgagatg acgaaggagc
     2821 tggagaatgc aaaagccgag gctgagcgca aagtggccga gcgcaccgag caggtgcgag
     2881 ccggttctgt cgtggctcgc gtggcagctt cgattctcga ccccgatcgc ctgctgcgcg
     2941 acgtcgtcaa cctgatcgcc gatcacttcc ggttcgacta cgcagcggtg ttcacactgg
     3001 acgcggaagg tcggtatgcc atgctgcgcg aggcgacggg cgatgtgggg cagatattga
     3061 agcagcgtgg ttacaggctg gagatcggcg ggcagtcgat gatcggcacg gcgattgcct
     3121 ggcgcaagcc gcgtctcgtg cttgacgtca gccgggagcc gatcctgcga agtcccccac
     3181 cgaggggagc gggaacaacg gatttggcga atgcagcggg aacaacggat ctggcgaatg
     3241 cagcggatcg gggggaaggg gcagccgaag gacgttttgc caatcttctc ctgccgaatg
     3301 cgcgctcgga gctggcgctg cccctgatgg tcggcgagcg cgtgctcggg gcgctcgacg
     3361 tgcaatcggc gcatgaggcc gccttcgacg aagccagcac gaccgtgttg cagagcgtgg
     3421 ccgaccaaat cgcgatcgcg cttaacaacg cggagcagtt ccaccgcgcc gagcggcaag
     3481 ccgcggccct ggcggtgctg aatcagctca gccgcgatct gacgatggct gcatcgctcg
     3541 aggcgatcgg ccacgcggct gtgacggcgc ttgccagtct cgtcgctgtc gacggtctcc
     3601 tgctgggact ggcgacgccg aatctcgaaa cggtcagaat cgtgccactc gatctgcaga
     3661 gccgggagca gcaggatagt ccccgaagcg aagccgaagc cctgaacgca gtgaaggggc
     3721 agtgggagtc gatcccaccc gccctttccg ctgctcaaac gttcgtcggc cagtgcattc
     3781 gcacgggaca gaatgtgctg gtggcagacc ttggcgcgct cgccagcgcc ccctatcaag
     3841 atgctgcctt ggcgcggaag gccggcatgc gttcactcat tggtgttccg ctgcaggtcg
     3901 gtggccagac cgtcggcgcg ctatttgctg gcgcggcgcg gctggatgcc tatgcacctg
     3961 acgatgtgag ccgcgtggag caggtcgccg cgcaactggc cgttgccgtc gagagcctca
     4021 atcgggcgga agacatgcgg caggccctgg aaggattgga tgctgccaat cgccgcctga
     4081 ccggccaggc atggtcggct ttcgcgcgcc gcttgagccg ccagggcgtg atcagaatag
     4141 gctcccatgt ggatgacaaa gcgccgtcgc ctgaagtaga ggaagtgatc tcaaccgggc
     4201 aactggtcgt gcaccctgcc aagtcctcga ccgaaggggc cggcgagggc tatcgcgtgg
     4261 cggtgccgat cgtgctgcgc ggtgtgccaa tcggggcatt tcggctgtcg gttccggagc
     4321 attcctggtc ggacgacctg gcagccaccc tggattcgat cgcgggccac gtggcgcagg
     4381 cggtagagaa cgcgcggctc gtggaggagg ctgaagaacg ggctgctcgc gaacgtgctc
     4441 tggccgatgc gacggagaga gtccgatcca aggcggacgt ggagcgcatt ctgcagaccg
     4501 cagccgaaga gctggcgcgc catctcaacg ccgccggcgt cgccgtccgg ttcgcccctg
     4561 gcgtgcatgg cagccccgac gccggtcagg agcgatcaat cgccgggaac ggcgcggagc
     4621 gggaggaagc atgacgacaa tccccaaagc gcagccgagc ttacacccaa cgctcaggcc
     4681 accgtccccg ccggctttgc ccgcgccgat gcctgccctg agccgaagcc ctagccgaag
     4741 tcctgaccct gaacgaagtg aaggggcagg ggcaggcgaa gggacggcga aggggcaggc
     4801 cctgctcaag acgggcttcg ccctgctggc atcgatcgtc acactcgcat cctgcagcgc
     4861 ccccgtctct ccggttctca caagccagcc tgccggtgga cgccagccgg cgggcgagca
     4921 ggccgccgag ctgaatatct tcaattggga tacatacatc gatccggcca tcctggacga
     4981 cttcgagcag aagtacagcg tgaagatcaa ctacgacatt tatgccagca acgaagatat
     5041 gctggccacc atccaggccg gcccgaccga ctacgacatc gtcgtgccat ccgactacat
     5101 ggttgcgatc atgcgcggcc aggacctgct cgccccgctc cgaaaggatc gtcttcccaa
     5161 tttcgagcat gtcgatgaag cctttctcaa tcccgtattc gatccgggca accgctactg
     5221 cgcaccgtat cagtggggta ccatcggcat cggctacaac gtcagggcca cggggcgcga
     5281 gatctcgagc tgggcggacc tcttcgatcc ggtcttcgcg gggcgtgtgg ccttgctgga
     5341 cgatgcacgc ggtgcattgg gagtcgtcct gctatatctg ggttacagcc ccaacaccac
     5401 gaatgtcgtc gaacttcaga cggcgcgcga ctttctcaag cagcgttcca ggcacatcgc
     5461 cgcgttcgtc ccagacacgg gccaggatct gctcgtcgct ggcgacgtcg acctggtcct
     5521 cgaatggagt ggagacatct tccaggtcat ggagaacaac ccggatattc gttacgtcat
     5581 cccgaaggaa ggctccatca tctggtcgga taacatgtgc atactgtcta gcgcgcccca
     5641 cccggacctg gccgagaagt tcatcaacta catcctggag ccggaagtcg gcgcagccct
     5701 atcttcgtac gttcgctacg cctcgcccaa tcgcgccgcc ttgcctttgc tgcccgcatc
     5761 cgatcgaaac gacccggcgc tttatccatc ggacgaagtg cgccagcgat tgttttttct
     5821 cgccgacaac ggcccggagg ctgcccggct cttcgagcag gcctgggccg acatcctggc
     5881 ggcgcacacg cgttgagtca tgaaaggccc tgagcatgtt gtctgagcct cgcgtcaagt
     5941 cggcccgccg cccggcaccc atccggaccc agttgtttgt aggcttcggg ctgatcctgg
     6001 tgctggccct actcattgcc gtgatcggct acgtgagcct gcgcagcttg cagtggggga
     6061 cccaggcgac gatcgaacag gccgagcata ttcgccagtt gagcctggcg atcgaaaggg
     6121 acttcctctc ggcgcgtcag agcgaggctc gctttcttaa tagctggcgc tccatcggcc
     6181 ccgaagccgc agccgagcgc tatatgaccg aagtacacga cttgctcgcg caagccaggg
     6241 ccaaactgga cgacctggat gctctgcttc tcgcggccgg cgatgagcag ttgagaagtt
     6301 tggtcgagca gacggctgca ttgcggcctc tgctggatac gtatgagacg gcattccatt
     6361 cgatggcgac cgatatccag aaacgcgccg acccagatat cggtctcgac cgcgcgttga
     6421 aaaatggcct cgacatgctg gaagcggacg tctcgcctct gcccaagacc gatctccttc
     6481 tcctcgtcct gcggctgcgc gcgaacgaac aagcctattt cggcgccggc gggcaagaat
     6541 actacgacaa cgtgagactg gccgtcgaca agttcgtcaa tctggtgaaa gccagcagcc
     6601 tagcagactt gagcacggaa acgacccgat tggtcccatc cgatctgatc gatcgggcac
     6661 aggcttactt ccagaccttt ggcgagctgg tcgccctgga gcaaggcata caggtgttca
     6721 cgacgatctt ccaggaggtt agctccgaca tcaatctcat cacggagcgc ctcaacgccg
     6781 aaggcgaatc cggcctggat cgcgcccgcg cccgcatgca aacgctcggc aacgagatca
     6841 ccgtggctct ggccgcgacg gcttctctcg ctttaggtct ggcgatggtc gtgtcttggg
     6901 tgctggctcg ccgcatcgtc gggccacttg ccgatctgag ccgggcggcg ctccagatcg
     6961 ggcgcggtaa cctcgacgtg cccttgcccg cctcgaagat tcgggagatt gccgacctgt
     7021 ccgagacaat ggagcaggtg gcggatcaac tgcgtgcgct gatcggctcg ctggaggcgc
     7081 gcgtcgccgc ccgcaccgag cagttgcgtg ccagcgccga cgtaggccgc gcggccgtct
     7141 cggtcctcga ccccgatcgc ttgctgtacg acgtcgtcaa cctgatcacc aatcgcttcg
     7201 gcttctatta cgccgccgtg ttcctgatgg acgagaaggg tcgatacgcg gtgctgcgcg
     7261 aggcgacggg ccaagcaggg cgcattctga aagagcgcgg gcacaaactg gaagtcggcg
     7321 gacgatcgat ggtcggcttc gtgaccgcgc agcgcaggcc gcgcatcgcg cttgacgtcg
     7381 gcgaagaggc gatgcgcttt gccaacccgc tgcttcccga tacgcgctcc gaaatcgcgc
     7441 tcccgctcgt cgtcggcgat cgggtgctcg gcgcgctcga cgtgcagtcg acccgggagg
     7501 cggccttcga cgaggctagc gcggccgtgc tccagggtat ggccgatcaa atcgcgatcg
     7561 ccctgaacaa cgccgcgctg tatgccgagt cgcagcgcaa cgtcaatacg atgaacgagc
     7621 tgcttgctgc gtcgagggac atcgcacgca gccgcacgct gccggagctg gccggcctcg
     7681 cgctgtcccg cgtccatgcg ctcatcggcg tcgacagcta ttacctgggc ctggtgaatg
     7741 agaatcagac cgagatgcgt ttcgtcgtcc gcgtgctcgg cggcgtcgac gtcagcgata
     7801 tggtggcggc acaccctctg ggcgacggct tacccgagca cgtgattcgc acccgcaagc
     7861 cgctgcgcat cagcgcttcc gaagcggcgc gcgccgcgca gtccggcatc ccggtagaag
     7921 aggcgcagcg gggcgcgttc ttgggcgtcc ccgtcgtcgt cggtgagcgg gtgttgggcg
     7981 tgctcggttt tcaggatatg cggcccgact ctttcttcag cgacgatcag gaaaggttgg
     8041 cggtcacgct cgcccgccaa attgctgtcg cgctggacaa cctgcgcctg gccgaggaaa
     8101 cccggcgggc gctcgccgac ctggatgcgg cgaaccggct cttgacgggc caggcgtggc
     8161 gccagtacgc gcgcgcagcg cgcggtctgt ccggcgaatg gcgggccggc gaatggcttg
     8221 ccctgagcga aggcgaaggg tcctctcgcg gcccacagga gagtgcagac ggtgtgcctg
     8281 gtcgcctgac cattccgctg cgcattcgcg gcgaggctgt cggcgagttc gacttgctgt
     8341 ctctggggga tggagaggga tggacgccgg aggatgtggc ttttgcccag tccctggtcg
     8401 atcaggtcgg tcagacgctg gagacggtgc gccttctgga agaaacggag cgcctggcgg
     8461 gacgcgaacg gctcatcaac gagatcaacg ctcgggtgcg ccagacggtc catatcgacg
     8521 gcatcttgca gacggccgtc gacgagctag gccgttctct gaaggctgcc cgcgtggtcg
     8581 cgcgcatcgg cgtgcccgag acttctacgc aagagacttc cgtgcacgca acttcaggga
     8641 cacgcgagcc aggagtgcgt gccgagcggg gcgcagcgga tccggagaag cgcgatggac
     8701 gaggtgacga tgatgcgtag cctgacatcg cgcctgcgaa cgtcgcttcg gttcatgggt
     8761 ctgcctgtgt atcctgacga ggaacgggcg cgggccgccc gtttgctcct ggcgattctg
     8821 tcgatcctgc tgatcaccgc cgtcgcggtg ctgatcgtca gcgtgcttga tggccagtct
     8881 cgcacggctc aaatcgtctt gaccggcagc atgatccttc ccttcgcgtt ctggttgctg
     8941 cgtcgaggcc atcttcaggc cgccagcctg tgtgtggtgc tggttctgct gagcgtgctg
     9001 acctacctgc tggtcgttgg cgaaggcatc cacgatatcg ccctgaccgc gtacccgact
     9061 gtcatcgtca tcagcagctt gctgctgggg cggcccggcg cagtgatcgg cacagtcttg
     9121 tcgatagtgg cggtgggctt ggtgttcgta gccgagagcc agggagtggt caccaacccg
     9181 ttcagttaca cgacgtcgcc cggcgaattc gcgatcctcg tagccctcct gtgcctggtc
     9241 agcgccatgg tcgagctgtt ggcgagtaat acgcgtcgca gcctgaagcg cgcccgccgc
     9301 aacgagcacg cgcaggtcga agccaaccgc gagctgcagg cgattcgcga ttctctggag
     9361 cagcgggtcg ccgagcgcac cgcacaactg agcgtgagcg ccgaggtgag ccgcgcagtc
     9421 acctccgtgc tcgatccgga cgatgtgatt cgccgggtcg cacagttgat cacggagcgg
     9481 ttcggtttct actacgccgc cgtgttcctg ctcga
//
LOCUS       DBNHXB010000115        14305 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_357368, whole genome shotgun sequence.
ACCESSION   DBNHXB010000115 DBNHXB010000000
VERSION     DBNHXB010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 14305)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14305)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14305
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_357368"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1159
                     /locus_tag="AC162G_04435"
     CDS             164..1159
                     /locus_tag="AC162G_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR5075094.1"
                     /translation="MPYALPSTATIDAGKTGGQGLNLRDAPGTTGRRLATMPINTRLT
                     IVGVARQGFNPNATALWYQVRYLWRIGFCAAGFLSLDAEPTVPLPVTATVDPQKTGGM
                     GLNLRALPVANGKFLATMPENAPVQIVGAAREGFAAEGGLWYHVQFGAQRGYCLSGFV
                     VTASIPGSVLTPTPPGPFVGVWPVEFPQRVITAPFNQPRPSFSSPVKAHEGVDLRAPN
                     GTPVMAWADGRVAMTWAWDGASRSGNHAYGHHVKMFHPALGLYSMYCHLQSIGVVKDQ
                     VVQMGERIGLADNTGNSTGAHLHFMIVDPVNGLNGYVYPKVVDPTPYLPQPYTIL"
     gene            1229..1723
                     /gene="mog"
                     /locus_tag="AC162G_04440"
     CDS             1229..1723
                     /gene="mog"
                     /locus_tag="AC162G_04440"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HIR5075095.1"
                     /translation="MIRVAILTVSDRSARGARPDASGPAIAQFVRDVLKWTIAQTAVV
                     PDDAAAICSTLTEWCDEAKVDLIFTTGGTGFSPRDVTPEATRAIIDREAPGLAEAMRT
                     ASLKVTPHAMLSRAVCGIRGHTLIVNLPGSPKACLENLETIAPALPHAIQLLGEDPAA
                     EAGH"
     gene            1746..2843
                     /locus_tag="AC162G_04445"
     CDS             1746..2843
                     /locus_tag="AC162G_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIR5075096.1"
                     /translation="MSQARHFGCPSCGGDLTVRGAEMQVRCPYCGRAVIVPEELRAQP
                     RRPTPEVAAPAAPATEYARRGISVAPVVMALLVIGLALFVINGLGLFDDLSGGEAPVA
                     PMNRALEGTLPRQTTYAGLQFNVTGATISNQVPHPFGDSPEYSRDQAYAYLDVTVNNP
                     TFDSIYVDSGLAQLRLSDRVYKEDGGWADIIDAQSTKEARWVFAVPFDSTWTRAELIL
                     AQPGKEPAKLPLEGSVPAPEYPIRLSPGGQATAQEVTYQILSAVLDLDAVGNRADVGK
                     RFLKLQMRFTNNSPFAGGFALAPNDFRLIVDGSPLAPIEAPIEVLAAQSVLEGEVIFD
                     VPASASEVALQVGEVGRGDMALIPIDLPPLP"
     gene            3018..4034
                     /locus_tag="AC162G_04450"
     CDS             3018..4034
                     /locus_tag="AC162G_04450"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR5075097.1"
                     /translation="MTNWPQAVAAAHATGKDDHSENGVTVRRIRGGANNALYRVDVEG
                     QSFACKLCIDDERQRAAREYGALELLHRAGLDLAPRPLWLDESFRIFPVPAVASSWLQ
                     GVPIGPALTMRQLANLLETIQATHAVCPNRWSHSPLPAAWLHWFDFDRYLIEMQSLID
                     TYGLWLMASDREGAALIERMQRVLARCAQIVAKTDVNPDYDHVPLCLCHVDPNLANAI
                     WCDDDRLRWVDWEYSGWGDPALALADLRWHAALEGLTDEQHAWLRENYRRPDDDPAFD
                     ERLRVWDCILATRWPLLIVRLLWSQHNGPDRPRLSQPAVELAEVRERFVRFIRRAEEH
                     LDWS"
     gene            4073..4249
                     /locus_tag="AC162G_04455"
     CDS             4073..4249
                     /locus_tag="AC162G_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075098.1"
                     /translation="MRVESATELGGKIAQMVDRRTGRKWPERHPRLAWCLPATRFLIT
                     ASAGIAGPTNDERA"
     gene            4414..6093
                     /locus_tag="AC162G_04460"
     CDS             4414..6093
                     /locus_tag="AC162G_04460"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HIR5075099.1"
                     /translation="MKRIIVKLGLIAVVGLGSAVMLLGLLNQRVLVARATGPILYVAP
                     DGSDTTDCTSSANPCATIQYAVDQADAGAEIRAAMGTYAGVSAREGVTQVAYMSKTVA
                     LRGGYTTTNWIEPYPITQPTTLDAQGQGRVLHISGGGITVTLANLHVTGGHANGTSYQ
                     GHGGGIYIAGASTWITNSAVTSNTATGFGGGISALSASLRLAGSTVAFNLAGVGGGGI
                     GLNYGTAIVDGSIVVSNTVPGPGTSAVGGGIAIARGEIVLNRNLIVSNTAGYGGGMAS
                     YFATSVILSDNLIVSNSADSGGGLGLHYSSVTLDRNTVQSNTARVEGGGLALDTSPAL
                     LSRNRILANVAQSCGGVCAGGSITVTLKDTAVLSNVATEYWAGGIGLFASHAILINTI
                     VADNSSPQGSALRIEASSVQLIHATIARNRNSPGLIVTGTVNQPSTVVLTNTILASHT
                     IGVMVSAGSTAKLDATLWGSGVWSNGLDWGGEGTIITGTVNVWGNPAFIDPDAGDYHI
                     GPGSAAIDAGVNAGVNVDIDGEPRPDGFGYDIGADEYTPRSRLYLPIVIRS"
     gene            6114..7466
                     /locus_tag="AC162G_04465"
     CDS             6114..7466
                     /locus_tag="AC162G_04465"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5075100.1"
                     /translation="MKQWQKLFYASGSLGTALSYQAFTTHVQFLYIDVFGLSAALIGT
                     GWAVYGIWNAINDPLAGSWSDRTRTRWGRRTPWIAATAAPVGLFFLLLWVPPESLVGV
                     GGDPLFAYFIVVVLIFDLLWTIVVMNWTALTPEMFVEDKERASVSGWREVFSIGGLIA
                     GVALMPILAGEGWANRGPVATLFALITSASFLLSLLGTRERKDIQSQPRVSFMQSLKA
                     ALSSAPFRWFLAANLSKEFVFSILAASMPFYAKYVLGASTGETGYVLGAAFVAAILGL
                     VLWTRYAKRAGARRAWQVCCITFSLSTLPFLFASDLASGLITATILGLSLAGYLMLPT
                     ILISDVTDADELETHTRREGMFFGINGFVIRFAFTLQGLALGVVLATTGYVQPLMPAD
                     TPAQPDAALWGMRALVTLLPIIASAITYWALVRYPLHGERLTEVRRRLAGANERRRMD
                     "
     gene            7472..9541
                     /locus_tag="AC162G_04470"
     CDS             7472..9541
                     /locus_tag="AC162G_04470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR5075101.1"
                     /translation="MKMGPFALLILTGTLLLAAVLRLNHLEWAEFKLDEANLSRLSLN
                     LARGVEIPLVGIGASTGIPNLPLAAWLMAIPYAVSSSPIVATGFVALLNVVAVAGCFA
                     LAKKWLSAYGQPESGHAAGIWTAAFLATLLFAVAPWAVIHSRKIWAQNLLPPLALVWA
                     WAGWLAFVRRRPRALIGHWLALAACIHVHYSGLWLLPVTLAWMIVFARRLKWRSALAA
                     VALFAATFALFVVADALNGAPNLNRLLLVAQGAPVVDGEALRLAWLMVTGQEIHSLAG
                     PQEFTNYLASVPGGETGFALVAGSGVLVVASAAIALVDVIRAARRRSLDEPAAAAFMM
                     LTWLALPVLLQTSHSLPIFIHYFIVLNPAPYLLMGTVYVRLAESLHDASRASSRGGRH
                     VASVVRWGFIALVVLVASLQALQTIALQQFVAGRATPGGFGTPVGTLAQIAADATAVS
                     QGLNGSEVLIYAEGDDPRTHEAPAVFDVLLPPDVPRRFIDLTRATEVYPRDAAAVVIY
                     SPQGLTVPGEAVRRTPLRRETSVVPLRRGDASARVGEWPGQTDGAWPCGGQRALGHWA
                     NGVALLSVDRSGAWRGPGGWIELCFRVDREPEQADYHWFTHLIGPGGQRYAQVDAPAL
                     PSAFWRPGDTVIARFGPYILPENAPAGLYVMRVGMYTYPNIVNVPRDDGGQPADYVEV
                     NLDNSLD"
     gene            9697..9927
                     /locus_tag="AC162G_04475"
     CDS             9697..9927
                     /locus_tag="AC162G_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075102.1"
                     /translation="MSQAATNETAQRRLNILRFGLMAVTAIAFALAAALAFFATLGNI
                     VTTVLIGLGAGIVVAIVSVLIYRVYKSAVLKA"
     gene            complement(10074..11225)
                     /locus_tag="AC162G_04480"
     CDS             complement(10074..11225)
                     /locus_tag="AC162G_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5075103.1"
                     /translation="MYFELTEEQKLLRRTVRDFAETELKPHARHVDEHAEFNAAAVKK
                     MGPLGLLGLSVPEEYGGAGVDAISAAIAIEEIGRGCGSTGLAIAAHNGLACAPLVLFG
                     NEEQKKKWLAPMASGSGGLGTLALTEPGAGSDLAGGVRTAARRDGDEWVINGSKMWCT
                     NAGLADVIVTLCRTDPAGGSHSLSLLIVPTAAPGLHIGPPEKKMGLKGSPTHAVTYEN
                     VRVPASNLLGAEGRGLQEALEVLDGGRIGIGALAVGLAQAAHEEAVKYAKERHTFGQP
                     IAQHQAIQWMIADAATRIDAARLMVYRAAWLKQMGRPCTTEAAMAKLFATEVAEKVCF
                     DAIQIHGGYGYSAEFPVERIYRDQRLMTIGEGTSEIQRLVIARDVLSPR"
     gene            complement(11263..11505)
                     /locus_tag="AC162G_04485"
     CDS             complement(11263..11505)
                     /locus_tag="AC162G_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075104.1"
                     /translation="MPVFCPHCGVMQPEGVAKCANCGKPMPPVAAPQAETPADEISPA
                     EMRAYVGVVLRYTVVPIVITVAVFCVAWLICINLIR"
     gene            complement(11528..13480)
                     /locus_tag="AC162G_04490"
     CDS             complement(11528..13480)
                     /locus_tag="AC162G_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5075105.1"
                     /translation="MEAFEFGGEIVWRPTPEHIANSNLKRFMDRHNIASFDELMRRST
                     SDIAWFWEAVLQELDIRFYTPYTQIVDLSRGIAWPEWCVGGVMNIVHNCLDKYAGTPD
                     DDRIALRWEGEEGSVRALTYAELRREVNKAANALRALGLSKGDAIGVFMPMTPEIVVA
                     LLAIVKIGGVFLPLFSGFGAQAIASRLADAGAKALFTADGMFRRGQIAPMKPVADIAA
                     AQVPTLKHVIVLRRTGGEIPWNAQRDDWWHELVDKQPEEAETERTRAEDPLMIIYTSG
                     TTGRPKGAVHTHCGFPIKSAQDMLHGLDLHPDETLYWITDMGWMMGPWLVFGTLLIGA
                     TMMLADGAPDFPGPDRTWALVEKHRITTLGISPTLVRALIRFGEEPVRAHDLSSLRKF
                     GSTGEPWNPDPWLWLFNVAGGGQLPIINYSGGTEISGGIVMGNVVLPLKPCAFSGPLP
                     GMAADVVDDEGRPVRGQVGELVIRQPWIGMTRGFWKDPQRYVGTYWSRFPDVWVHGDW
                     AAVDADGLWYILGRSDDTIKVAGKRLGPAEVESILVAHPAVSEAAAIGVPDPIKGQAV
                     VCFAVLKPGQSPEEALREELMELVAGALGKPLAPREIKFVRDLPKTRNAKVMRRVIRA
                     AYLGQEPGDLSSLENPAAVEEIRRAV"
     gene            complement(13615..14103)
                     /locus_tag="AC162G_04495"
     CDS             complement(13615..14103)
                     /locus_tag="AC162G_04495"
                     /inference="COORDINATES: protein motif:HMM:NF047646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase"
                     /protein_id="HIR5075106.1"
                     /translation="MPHRYQVFSDSHYAYFVTCTIVDWLPVLAHDAYRQIVLDSLAHL
                     REHKQTQLNAFVVMPTHLHAVLWPQEGIILSDVLRDFKRFTSRSISKEATRRGDHQFL
                     DVFAAVRQQGRAQNTSQYQVWQEGSHPEAIYTLDFAHQKINYIHANPVRAGLASAPED
                     WS"
BASE COUNT         2427 a         4471 c         4650 g         2757 t
ORIGIN      
        1 agcagcgcta cgacctggcg cagcgccagc ccgatggctc gatacgtatc tattacggcg
       61 tcaccgaaga cggcatccac ggcccgtggc gtaggctggt cgggctggag gattgactcc
      121 aaatcccaaa ctccaaactt caaactccaa agtggaggca cgtatgccct acgctcttcc
      181 atcgaccgcg acgatcgacg ccgggaagac cggcggccag ggtctgaacc ttcgggatgc
      241 gcccggcacc accggtcggc gcctagcgac gatgccgatc aatacacgcc tgaccatcgt
      301 cggcgtggcg cgccagggat tcaatccgaa cgcgacggcg ctgtggtacc aggtgcgcta
      361 cctgtggcgc atcgggttct gcgcggcagg cttcctctca cttgacgccg agccgaccgt
      421 cccgctccct gtcacggcga cggtcgatcc gcagaagacc gggggcatgg gacttaatct
      481 gcgcgcgttg cccgtagcga acggcaagtt cctggcgacg atgccggaga acgcgccggt
      541 tcagatcgtg ggcgctgcgc gcgaagggtt cgcggcagag ggcgggttgt ggtatcacgt
      601 ccaattcggc gcgcagcgcg gctactgctt gagcggcttc gtcgtgaccg cctccattcc
      661 ggggagcgtg ttgacaccaa cgccccccgg cccgttcgtc ggcgtgtggc cggtggaatt
      721 tccgcagcgt gtcatcaccg cgccgttcaa ccagcctcgg ccctcgttct cctctccggt
      781 caaggcgcac gagggtgtcg atctgcgcgc gccgaacggc acgcccgtca tggcctgggc
      841 cgacgggcgc gtcgcgatga cgtgggcatg ggacggcgcc agccggtcgg gcaatcacgc
      901 ctacggccat cacgtgaaaa tgttccaccc ggcgctcggc ctgtactcga tgtactgcca
      961 cctgcagtcg atcggcgtcg tcaaagacca ggtggtgcag atgggcgagc ggatcggatt
     1021 ggccgataac accggcaaca gcaccggcgc gcatttgcac ttcatgatcg tcgatcccgt
     1081 caacggcctg aacggctacg tataccccaa ggtcgtcgat cccactccgt accttccgca
     1141 accgtacacg attctctaac tcgcgcaagg cagggcaggc gcaaggcctg cccccacgtg
     1201 gattcggaat gatcaacgga ggctgtctgt gatccgggtg gccatactca cggtgagcga
     1261 ccgctccgcg cgcggcgcgc ggccggacgc cagcgggcca gccatcgcgc agttcgtgcg
     1321 cgacgtgctg aaatggacta tcgcgcagac cgccgtcgtg cccgacgacg cggcagccat
     1381 ctgctcgacg ctgaccgaat ggtgcgacga agcgaaggtc gacctgatct tcacgaccgg
     1441 gggcaccggc ttctcgccgc gcgacgtgac gcccgaggcg acgcgcgcga ttatcgatcg
     1501 cgaagcgccc ggcttggctg aggcgatgcg caccgccagc ctcaaggtca cgccacacgc
     1561 catgctgtcg cgcgcggtgt gcggcattcg cggccatacc ctgatcgtca acctgcccgg
     1621 ctcgcccaaa gcctgcctcg aaaacctgga gacgatcgcg ccggcgctgc cgcacgcgat
     1681 acagttgctg ggtgaagacc cggcggctga ggcggggcat tgattgccgg agggaggagc
     1741 attccatgtc gcaggccagg cattttggct gtccatcatg cggcggagac ctgaccgtgc
     1801 ggggcgcgga gatgcaggtc agatgccctt actgtggcag ggccgtcatc gtgccggagg
     1861 aactgcgcgc ccagcctcgc cggccgacgc cggaggtcgc cgcccccgcc gctccggcaa
     1921 ccgaatacgc gcgccgcggg atttccgttg caccggttgt gatggcgctg ctggtgatcg
     1981 gccttgccct gttcgtgatc aacggcctgg gactcttcga cgatctgtcg ggcggagaag
     2041 cgccggtcgc gccgatgaat cgcgccctgg agggaacgct gccgcgtcag acgacgtatg
     2101 cgggactcca gttcaacgtg accggagcga cgatcagcaa ccaagtgcct cacccgttcg
     2161 gcgattcccc ggagtacagc cgtgatcagg catacgctta cctcgatgtg acggtcaata
     2221 atccgacatt cgacagtatc tacgtcgatt cgggcctggc tcagttacgg ctcagcgaca
     2281 gggtctacaa agaggacggc ggctgggccg acatcatcga cgcgcagtcc acgaaggaag
     2341 ccaggtgggt ctttgccgtg ccgttcgatt cgacgtggac gagggccgag ttgatcctgg
     2401 cgcagcccgg caaagagccg gcgaagctgc cgctcgaagg gtctgttccc gcgcccgagt
     2461 atccgatccg attatcgccc ggcggccagg caaccgcaca agaggtcacc taccaaatcc
     2521 tttcggctgt gctcgatctc gatgccgtag gcaatcgtgc ggacgtgggc aagcgcttcc
     2581 tgaaactcca gatgcgcttc acgaacaaca gcccattcgc gggcggtttc gcactggcgc
     2641 cgaatgactt tcgcctgatt gtggatggct cgccactcgc gccgatcgaa gcgccgattg
     2701 aggtgcttgc cgctcaaagt gtgctggagg gcgaggtgat tttcgatgtg cccgcgtcgg
     2761 cgtcagaggt cgcgcttcag gtcggcgagg ttggcagagg ggatatggcg ctcatcccga
     2821 ttgatctccc accgctgccg tagaaaaatg gcggctaaga aagccgccct gcgtgctgac
     2881 gcaggccatt gatcttcgaa cgctgcctgc atgctctgat agtgccgaca gatatcccgg
     2941 tgaagacccg cgccgacgga ctctatacgt tcgtgctttc tttgctacct gaggaagtac
     3001 atccataagt gaatgggttg acaaactggc cgcaagcggt tgccgccgcg catgctaccg
     3061 gaaaggacga tcattctgag aacggcgtga cggtgcgccg catcaggggc ggcgcgaaca
     3121 atgccctgta tcgcgtcgac gtcgagggcc aatccttcgc ctgcaagctg tgcatcgacg
     3181 acgagcgcca gcgggcggcg cgcgaatatg gcgcgctcga actgcttcac cgtgccgggc
     3241 tggacctagc gccccggccg ctgtggctcg acgaaagctt ccgcatcttc ccggtccccg
     3301 cggttgcatc ctcctggttg cagggcgttc ccatcggtcc agcgctaacc atgcgccagc
     3361 tcgcgaatct gctggaaacg attcaagcga cacacgccgt ctgcccgaac aggtggtccc
     3421 attctcctct tccggctgca tggcttcact ggttcgactt cgaccgttat ctgattgaga
     3481 tgcagagcct tatcgatacc tatgggctgt ggctcatggc gagcgatcgt gagggagcgg
     3541 cgctgatcga gcggatgcag cgcgtccttg cacgctgcgc gcaaatcgta gcgaaaaccg
     3601 atgttaaccc cgactatgac cacgtgccac tgtgcttgtg ccacgtcgat cccaatctgg
     3661 ccaacgcgat ctggtgtgac gacgatcgac tccgttgggt cgattgggag tacagcgggt
     3721 ggggcgatcc ggcgctcgct ctcgcggacc tgcgctggca cgctgcgctt gagggcttga
     3781 cggacgagca gcacgcctgg ctgcgcgaga actatcgccg ccccgacgac gatccggctt
     3841 tcgacgagcg gctgcgagtg tgggattgca tcctcgctac acgctggccg ctgctcatag
     3901 tgcgcctgct gtggagccag cataacggtc cggatcggcc gaggctgagt cagcccgcgg
     3961 tggagctagc ggaagtgcgt gagcgattcg tccgattcat ccggcgagcc gaagagcatc
     4021 ttgactggtc ataaaatcgc cgattcacgt gacatgcctc gataacccgg ctatgcgggt
     4081 cgagtctgcc acggagttgg gtggcaagat cgcgcagatg gtcgatcgac gcaccgggcg
     4141 caaatggccg gagcgccatc cgcggctggc gtggtgcttg ccggctactc gcttcctgat
     4201 cacggcgagt gctggtatcg ccggcccgac gaatgacgag agggcttgac gtcggaacat
     4261 cgccagcgtg gggttacact accgttcgaa cttcggcgcc tcgttacgct cgatcgggat
     4321 cgaccactta tcacgctcga ttgtgcgctg cgctcatcgg ccgatacgcc gatctggctg
     4381 atcctggttg gggcagtagg aagaaggtga gacttgaagc gcatcatcgt caaactcggc
     4441 ctgatagcag ttgttggact gggatcggca gtcatgctgc tggggctttt gaatcagcgt
     4501 gtgcttgtgg cgcgcgcaac gggaccgatc ctttacgtcg cgccggacgg cagcgacacc
     4561 acagactgca cgagtagcgc caatccctgc gcgacgattc agtatgccgt ggatcaggca
     4621 gatgcaggtg cggagatccg cgcggctatg ggaacctacg ccggcgtaag cgcacgcgag
     4681 ggtgtgactc aggtagccta catgagcaag acggttgcgc ttcggggtgg ctatacgacg
     4741 acgaattgga tcgagccgta tcccatcacg cagccgacca cgctggacgc gcaagggcag
     4801 ggacgcgtgc tgcacatcag cggtggaggc atcacggtaa cgctggcgaa tttgcatgtg
     4861 acaggtggtc atgcaaatgg aacgagctat cagggccatg gtggtggcat ctacattgcc
     4921 ggtgcttcca catggattac caacagcgcc gtgaccagca ataccgcaac cggctttggc
     4981 ggtggaattt ctgcgctcag cgctagcttg agacttgcag ggagcactgt cgcgttcaac
     5041 ctcgccggag tcgggggcgg cggtattggg ctcaactatg gtacggccat agtggatggc
     5101 agcatcgtgg tttcgaacac agttcccggc ccgggaacgt cggccgtagg tggcgggatt
     5161 gcgatcgctc gcggtgagat tgtgctcaat cggaatctga tcgtgtcgaa cacagccggc
     5221 tacggcggtg ggatggcctc atactttgcc acaagtgtca tccttagcga caatctcatt
     5281 gtttcgaatt ccgccgatag tggaggtgga ttgggcctgc actacagtag cgtgacactt
     5341 gatcgcaata ccgtgcaatc gaatacggcg cgtgttgagg gcggcgggct ggctctagac
     5401 acgagtcctg ctctcttaag caggaacagg atactggcca acgttgccca atcatgcgga
     5461 ggggtttgcg ctggcggtag catcacagtg accctcaagg acacggccgt cctctccaat
     5521 gtggcaacgg aatattgggc cggtggaatc ggactgtttg ccagccatgc tattctcatc
     5581 aatactatcg ttgccgataa ttctagtcct cagggaagcg cactgcgtat cgaggcctcc
     5641 tctgtgcagt tgatacatgc gaccatcgct cgtaatcgaa acagcccagg cctcatcgtc
     5701 actggaactg tgaaccagcc gagcaccgtt gtgctgacca acacgatatt ggcaagccac
     5761 acgatcggtg ttatggtttc ggcaggcagc acggccaagc tcgatgcgac gctctggggg
     5821 agtggggtct ggtcgaatgg cctcgactgg ggcggcgagg gcacgatcat caccggcacg
     5881 gtcaacgtgt ggggcaaccc ggcgttcatc gacccggatg caggtgatta ccacatcggc
     5941 cctggcagcg cggcgattga cgcgggcgtg aacgcaggtg tcaatgtcga catcgacggc
     6001 gagccgcgcc ctgacggctt cggctacgac atcggcgccg acgagtacac gccgagatcg
     6061 cggctgtacc ttccgatcgt cattcgttcg tgatcctctt cggagcctgg tgcatgaaac
     6121 agtggcaaaa actgttctac gcctccggtt cattaggcac ggcgctctcg taccaggctt
     6181 tcacgacgca cgtccagttt ctgtacatcg acgtctttgg gctgtccgcc gcgctgatcg
     6241 gcacgggctg ggcagtctac ggcatctgga acgcgatcaa cgatccactc gccgggtcgt
     6301 ggtcggaccg cacgcgcacg cgctgggggc ggcgcacgcc gtggatcgcg gcgacggccg
     6361 ccccggttgg gctgtttttc ctgctgctgt gggtcccgcc tgagtcgctc gtcggcgtgg
     6421 gaggggatcc cctattcgcg tacttcatcg tcgtcgtgtt gatcttcgac ctgctgtgga
     6481 cgatcgtcgt gatgaactgg acggccctga cgccggagat gttcgtcgag gacaaagaac
     6541 gcgcctcggt gtcaggatgg cgagaagtgt tctcgatcgg cggcttgatt gcaggcgtgg
     6601 cgctcatgcc gatcctggcc ggggaggggt gggcaaatcg cggcccggtc gcgactttat
     6661 tcgcgctcat cacaagcgcc tctttcctgc tgtctctgct tggcacgcgc gagcggaagg
     6721 atatacagag ccagccgcga gtctctttca tgcaatcgct caaggcggcg ctgtcgagcg
     6781 cgccgttccg ctggttcctc gcggccaacc tgtccaagga attcgtgttc agcatcctgg
     6841 cggcgtcgat gccgttctac gccaagtacg tgctgggcgc gtcgaccggg gagacgggct
     6901 acgtgctcgg cgcggctttc gtcgcggcga ttctcggcct ggtgttgtgg acgcgctacg
     6961 ccaagcgtgc cggggcgcgc cgggcgtggc aggtgtgctg catcaccttc agcctgtcga
     7021 cgctgccctt cctgttcgcc tcggacctgg cgagcgggct gatcaccgcc acgatcctcg
     7081 gcttgagcct ggccggctac ttgatgctgc ccacgatact gatctccgac gtcaccgacg
     7141 ccgacgagct ggagacgcat actcgccgcg agggcatgtt cttcggcatc aacggcttcg
     7201 tcatccgctt tgcgttcacg ctgcagggtc tggcgctggg tgtcgtgctg gcgacaaccg
     7261 gctacgtcca gccgctcatg ccggcggata cgccggcgca gcctgatgcg gcgttgtggg
     7321 gcatgcgcgc gttggtcacc ctcctgccaa tcatcgcctc ggcgatcact tactgggcgc
     7381 tggtgcgcta tcctctgcac ggcgagcgcc tgaccgaagt caggcgacgg ttggcggggg
     7441 cgaatgagcg tcgacgaatg gattgactga aatgaagatg ggtccattcg cgctgctcat
     7501 tctcaccggc accttgctgc tggctgcggt cctgcggctg aatcacctcg aatgggccga
     7561 gttcaagctc gacgaggcga acctgtcgcg gctgtcgctc aatctggcgc gtggcgtaga
     7621 gatccccctc gtcggcatcg gcgcgtcgac aggcatcccg aacttaccgc tggccgcctg
     7681 gctgatggcg atcccgtatg cagtctcctc cagtcccatc gtcgccaccg ggtttgtcgc
     7741 gctcctgaat gtggtcgccg tagcgggatg cttcgcgctg gcgaagaaat ggttgtcggc
     7801 atatgggcag ccggaatccg gccatgccgc tggcatctgg accgcggcct tcctcgcgac
     7861 gcttctcttc gccgtcgcgc cctgggcggt catacactcg cgcaagatat gggcgcagaa
     7921 cctgctgccg ccgctcgccc tcgtgtgggc gtgggccggc tggctggcct tcgtgcggcg
     7981 ccggccgcgc gctttgatcg gccactggct ggcgctcgcg gcgtgcattc acgtgcatta
     8041 ctccgggttg tggctgctgc cggtgacgct ggcctggatg attgtcttcg cccggcgcct
     8101 gaagtggagg tccgcgctgg cggcggtggc gctcttcgcc gcgactttcg cgctgttcgt
     8161 cgtcgccgac gctttgaatg gagcgccgaa tctcaatcgc ctgctgctcg tcgcgcaggg
     8221 agcgccggtc gtcgacggcg aggcgctgcg cctggcatgg ctgatggtca ccggccagga
     8281 gattcactcg ttggccgggc cgcaggaatt cacaaactat ctggcgagcg ttccgggagg
     8341 cgagaccggc ttcgcgctcg tcgcagggag tggcgtgctg gtcgtcgcca gcgcggcgat
     8401 cgcgctggtc gacgtgattc gtgccgctcg ccgccgctcg ctcgacgagc ctgcggcggc
     8461 ggcgttcatg atgctgacgt ggctggcgct tccggtgctg cttcagacgt cccacagcct
     8521 gccgatcttc atccattact tcatcgtgct gaatcctgca ccttatctct tgatgggcac
     8581 ggtgtacgtc aggctggcag agtcgttgca cgatgcgtcg cgtgcgtctt ctcgtggcgg
     8641 ccggcatgtc gcttctgtgg tgcgctgggg gtttatcgcg ctcgtcgtgc tcgtcgccag
     8701 cttgcaggcc ctgcaaacga tcgcgctgca gcagttcgtc gccgggcgcg ccacgccggg
     8761 cggtttcggc acgccggtcg gaacgttggc gcagattgca gccgacgcga ctgccgtcag
     8821 ccagggcctg aatggctcag aagtgctcat ttacgccgaa ggcgacgatc cgcgcacaca
     8881 cgaagcgccc gccgtcttcg acgtgctgct cccgccggat gtgccccgcc gcttcatcga
     8941 tttgacacga gcgacggagg tctatccacg cgatgcggct gccgtcgtga tttattcgcc
     9001 gcagggcctg acagttccag gtgaggcagt caggcgaacg ccgctgcgcc gggaaacctc
     9061 agtcgtgccg ctgcgccgtg gcgatgcctc ggcgagggta ggggaatggc cgggacagac
     9121 cgatggggcc tggccgtgcg gcggtcagcg tgcgctgggg cactgggcga atggagttgc
     9181 tctgttaagc gtggatcgtt ccggggcgtg gcgcgggccg ggcggctgga tagagctatg
     9241 ctttcgagtc gaccgcgagc cagagcaggc cgactatcac tggttcactc acctgatcgg
     9301 gcctggcggg cagcgttatg cgcaggttga cgcgccggct ctgccttctg ctttctggcg
     9361 ccctggcgac actgtgatcg cgcggtttgg gccgtacata cttcctgaaa acgcgccggc
     9421 cggcctgtac gtgatgcgcg tggggatgta tacatatccc aatatcgtca acgttccacg
     9481 ggacgacggc ggtcaaccgg ccgattatgt ggaggtgaat ttggacaatt ccctcgactg
     9541 agacctggcc ggtggaaacg gatctgtgac ttatttgaaa cgttattaac gaaatgcata
     9601 ttgaatatga catgcctcct gtgctagaat cgtcgcagtt tcgcgtgctc tggagcgggt
     9661 ccgagccaat tcaaacgtca gaaggagatt ggagacatgt cacaagcggc tactaatgaa
     9721 actgctcagc gtcgtctgaa catccttcgc tttggcttga tggcggttac ggcaatcgcc
     9781 tttgcgctgg ctgccgcgct cgcgttcttc gcgacactcg gcaacatcgt gacgacagtg
     9841 ctgataggcc tgggcgccgg cattgtcgtg gctatcgtgt ccgtcctgat ctatagagtc
     9901 tacaaatcgg cggtcctcaa ggcctgacgc gctccgacac gactcgacgg cgatgagcgt
     9961 aacgccctca tcgccgtctc gtttaatgcc cacctcccgc gcaccggggc aagggcaagg
    10021 ctaatgttct tgaaagtacg gtggccgcga ccaggccggc cgcggcgcga cggtcagcgc
    10081 gggctcaaca cgtcgcgcgc aattaccaga cgttgaatct cgctggtgcc ttccccgatc
    10141 gtcatcaggc gctggtcgcg gtagatgcgc tcgaccggga attcggccga gtagccgtat
    10201 ccgccgtgaa tctgaatcgc gtcgaagcat actttctccg caacctcggt ggcaaacagc
    10261 ttggccatag cggcttcggt cgtgcagggg cgccccatct gcttcagcca cgccgcgcgg
    10321 tacaccatca ggcgcgccgc gtcgatacgc gtggcagcgt cggcgatcat ccactggatg
    10381 gcctgatgct gcgcgatggg ctggccgaac gtgtggcgct ccttggcata tttcacggct
    10441 tcctcgtgcg cagcctgcgc cagcccgacg gcgagcgcgc cgatcccaat gcgcccgccg
    10501 tcgagtacct ccagcgcctc ctgaaggccg cgcccttccg cgccgagcag gttcgacgcc
    10561 gggacgcgca cgttctcgta cgtcacggcg tgcgttggcg atcccttcaa ccccatcttc
    10621 ttctcgggcg ggccgatgtg caggccgggc gcggcggtcg gcacgatgag cagagacagc
    10681 gagtggcttc ccccggccgg atcggtacgg caaagagtga caatcacgtc ggcgaggccg
    10741 gcgttcgtgc accacatctt tgagccgttg atgacccatt cgtcgccgtc gcgccgggcg
    10801 gcagtcctga cgccacctgc caggtcgctt cccgcgcccg gctcggtgag cgccagcgtg
    10861 cccagccctc ccgacccgct ggccatcggc gcaagccact tcttcttctg ctcctcgttt
    10921 ccgaacagca cgagcggcgc gcaagccagc ccattgtgag ccgcgatggc caggccggtg
    10981 cttccgcagc cgcggccgat ttcttcgatg gcgattgcgg cgctgatcgc gtcgacgcct
    11041 gcgccgccgt actcctccgg cacgctcaat cccagcaggc cgagcggccc catcttcttc
    11101 acggcggcag cgttgaactc ggcgtgctcg tcgacgtgcc gggcgtgcgg cttcagctcg
    11161 gtctcggcga aatcgcgcac ggtgcgccgc agaagctttt gctcctcggt gagttcaaaa
    11221 tacatggggt gaatgctcct cgtgtgaaat ggtcttcccc cctcagcgaa tcagattgat
    11281 gcagatcagc caggcgacac agaacacggc gaccgtgatg acgatcggca cgacggtgta
    11341 gcgtaacacc acgccgacgt aggctctcat ctcggccggg ctgatctcgt cggccggcgt
    11401 ctcggcttgg ggcgcggcga ccggcggcat cggcttgccg cagttcgcgc acttggcgac
    11461 gccttcgggc tgcatgacgc cgcagtgcgg gcaaaacacc ggcatggtcg actcctcggc
    11521 gaggcggtca gacggcgcgc cggatttcct ccacggcggc gggattctcg agcgacgaga
    11581 gatcgcccgg ctcctgtccc aggtaggccg cgcggatcac gcggcgcatg accttcgcgt
    11641 tgcgcgtctt cggcaggtcg cgcacgaact taatttcgcg cggcgccagg ggcttgccca
    11701 gcgcgccagc caccagttcc atgagttcct cgcgcagcgc ctcctctgga gattggcccg
    11761 gcttgagcac ggcgaagcac accaccgcct gtcccttgat cggatcgggc accccgatcg
    11821 ccgccgcctc gctcacggcg gggtgtgcga cgaggatcga ctcgacctcc gccggcccca
    11881 gccgcttccc ggcgactttg atcgtgtcgt cggagcgtcc caagatgtac cacagcccgt
    11941 ccgcgtcgac ggcggcccag tcgccgtgca cccacacgtc tggaaagcgc gaccagtagg
    12001 tgccgacata ccgctgcggg tctttccaga agccacgtgt catcccgatc cacggttggc
    12061 ggatgaccag ctcgccgacc tggccgcgca ccgggcggcc ctcgtcgtcg actacgtcgg
    12121 cggccatgcc cggcagcggc ccggaaaagg cgcacggctt gagcggcagg acgacgttgc
    12181 ccatgacgat accacctgag atctcagtgc cacccgagta attgatgatg ggtaattggc
    12241 cgccgccggc gacgttgaac agccacagcc acggatcggg gttccacggc tcgcccgtcg
    12301 atccgaactt gcgcagcgac gacaggtcgt gcgcgcgcac cggctcctcg ccgaacctga
    12361 tcaaggcgcg caccagggta ggggagatgc ctagcgtggt gatgcggtgc ttctcaacca
    12421 gcgcccacgt tcggtcgggg ccggggaaat cgggcgcgcc gtcggccagc atcatcgtcg
    12481 cgccgatcag gagcgtgccg aacacgagcc aggggcccat catccagccc atgtcggtga
    12541 tccagtacag cgtctcgtcg ggatgcaagt caaggccgtg gagcatgtcc tgtgcggatt
    12601 tgatcgggaa gccgcaatgc gtatgaactg cgcctttggg cctgccggtg gtgcccgagg
    12661 tgtagatgat cattaggggg tcttccgcgc gcgtgcgctc cgtttcggcc tcttccggct
    12721 gcttgtcgac cagctcgtgc caccagtcat cccgctgcgc attccacggg atctcgccgc
    12781 cggtgcggcg gaggacgatg acgtgcttca gcgtgggcac ctgcgcggcg gcgatgtccg
    12841 cgaccggttt catcggcgcg atttgcccgc gccggaacat gccgtcggcg gtgaacagcg
    12901 ctttggcgcc ggcgtcggcc agccgcgagg cgatggcctg cgcgccgaac ccggagaaca
    12961 ggggcaggaa aacaccgccg atcttgacga tcgccagcag cgccacgacg atctccggcg
    13021 tcatgggcat gaacacgccg atcgcgtcgc ccttgctcag gccgagtgcg cgcagggcgt
    13081 tcgccgcttt gtttacctcg cgccgcagtt cagcgtaggt cagcgcgcgg acgctcccct
    13141 cttctccttc ccagcgcagc gcgatgcggt cgtcgtccgg ggttccggcg tacttgtcga
    13201 gacagttgtg gacgatgttc atgacgccgc cgacgcacca ttccggccag gcgatgccgc
    13261 gcgagaggtc tacgatctgc gtgtacggag tgtaaaagcg gatatcgagt tcctggagaa
    13321 cggcctccca gaaccaggcg atgtcgctcg tcgagcgccg catcagctcg tcgaacgagg
    13381 caatgttgtg ccgatccatg aatcgcttga ggttgctgtt ggcaatgtgc tcgggcgtcg
    13441 ggcgccagac gatctcgccg ccaaattcga aagcttccat ctgacttctc tccacgaagg
    13501 aatgtcttgt tgggacggca gttgcactgc cgtccccttc gcgtctcaat acgtcggtaa
    13561 gatgtcgacc ggcggataag ttccttcgcc taaaagatat gcgcgagcac tggactatga
    13621 ccagtcctca ggcgcacttg cgagacccgc tcgcaccggg ttggcatgga tatagttgat
    13681 cttctggtgg gcgaagtcca gcgtgtaaat ggcttcaggg tgcgatccct cctgccacac
    13741 ctggtactgc gatgtatttt gcgcgcggcc ttgctgccgg acagcagcaa agacatccag
    13801 gaattggtga tcacctcggc gggtagcctc tttagagata gatcgagagg taaatcgttt
    13861 gaagtcgcgc agcacatcgg agagaatgat gccctcttgg ggccagagta cagcatgaag
    13921 gtgagttggc atgacgacaa aggcattgag ttgagtttgc ttgtgctcgc ggaggtgggc
    13981 caggctgtcc aacacgatct ggcggtaagc atcatgagcg aggacaggca gccaatccac
    14041 gattgtgcat gtgacgaagt aggcgtaatg ggaatcggag aagacctgat agcggtgtgg
    14101 catatttcag attgttgttg gcaccgcaat tgcagcattg ttggcacagc atttgtactg
    14161 ttgtgcccag cgtccggcag tgcaactgcc ggacgaactt gaggcaaagc cggacgaacg
    14221 tggtgcagcc tggtgttggc acagcagttg gactgctgtg ccccggcccc ggcgttcggc
    14281 agtgcaactg ccagacgaac atgag
//
LOCUS       DBNHXB010000116         4353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3209162, whole genome shotgun sequence.
ACCESSION   DBNHXB010000116 DBNHXB010000000
VERSION     DBNHXB010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3209162"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..178
                     /gene="tatA"
                     /locus_tag="AC162G_04500"
     CDS             5..178
                     /gene="tatA"
                     /locus_tag="AC162G_04500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR5075107.1"
                     /translation="MHLGGPELLIILVIVLLIFGVGRISRVGKELGTGIREFRKGISE
                     EQTESGKSEEQQK"
     gene            complement(272..997)
                     /locus_tag="AC162G_04505"
     CDS             complement(272..997)
                     /locus_tag="AC162G_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013125.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HIR5075108.1"
                     /translation="MSWRRAALFVAMNVIISATVTLLILSAWDARRPAAPSPVSVAQA
                     TPVLAAPASTPLEKPTVALTPTARGPFTYAIQSGDTLGDLSLRFDVSLEGLLAANDLS
                     EDAILSVGQEIIIPAGGSTEIAPPATTAAKTPATSGPALVVIREIEEPGSLNGETVIL
                     TNLGEVINLTGWTLSDGRTNRYTFPDVTLFPDAEINVHTRTGTDTPSDLYWGASDAIW
                     GATGTVAYLRDASGKLVATYRVP"
     gene            1129..2283
                     /locus_tag="AC162G_04510"
     CDS             1129..2283
                     /locus_tag="AC162G_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075109.1"
                     /translation="MKRTLTRRQLLNLAGASAIAAFLDACSAPAPEQATATPSGSTGY
                     RAYLPNVIAETTPTPAPKPTAKPSSPEPTPVPRRTFPAFARPSKLGIAVQRFRYPQIM
                     DRIIADGHPRVVKIFEDLGAAAEIKRRSPNTIVVGHIHQAYDFQNAITSGATDMAAYA
                     ADFVARHLSQYQVNTGVDYWEGHNEPVFENEAKMALYGQFEAERVRQMTAYGFKCAVG
                     NFSAGNPPFEQWDEFFPALRAIREYGGALALHEYSAPTLDYGYDPSSDDGSLTLRYRR
                     VYRQVIPADLHVPILITEAGIDGLVSADRIGAGWQDFIWYWNKIPLDPDAFWAYMDQL
                     QWYDEELQKDDWVIGATLFVAGGQAHFESYELVGEMGELLTQYWMAHPVG"
     gene            2352..3227
                     /locus_tag="AC162G_04515"
     CDS             2352..3227
                     /locus_tag="AC162G_04515"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR5075110.1"
                     /translation="MDDLERFWSNIFSEDAARIGEAWATLSAGEREAIAGHLRSIVTD
                     SDRPPEQRHAASIALSVASGKSAPTILLASGSPRRRELIALLGLPFGTTPADVDETPH
                     SGEEPAAMVVRLSLTKAESARATTRPSDYPTTRPILLAADTTVSLDGEILAKPTTAVE
                     ARSMLTRLRGRSHQVYTAITLINLETGEQITDLATTEVPMRDYSDAEMEAYIASGDSL
                     DKAGGYAIQHSGFNPVAKMSGCYANVVGLPLCHVTRSLRALGVEPPVDVPSACQEHLD
                     YECPVFQAILARRET"
     gene            3311..>4353
                     /locus_tag="AC162G_04520"
     CDS             3311..>4353
                     /locus_tag="AC162G_04520"
                     /inference="COORDINATES: protein motif:HMM:NF015662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2-like N-terminal transpeptidase
                     domain-containing protein"
                     /protein_id="HIR5075111.1"
                     /translation="MPKRLLPFIVALATACSGPTSTPTIRLSTATPLPPPPEQTALAY
                     FDAWQRADYAGMYELLTPSAQLAVNENQLASRYSQAHATATVKFVRATLKSALRDGSR
                     VTVDYRLEWDTVLFETLITNYSLTLVVDPVRNQWGVDWNDGLIWPALEGGNILSLNYT
                     IPRRANIYDVDGKGLAVEGKVVTVGVVPGQVADRATLIPQLSAILNLSLDFIESQIAS
                     AQPEWYVPLANISFDAFQASRSILEQPGVELREKAAREYRGGGAHAIGYVGFISPEEI
                     EAWKARGYQGDELIGKAGLEQWGETILAGTHGVTLNAITPDGQIVKEVAVRPAAPSRS
                     IYLTIKRDFQQQV"
BASE COUNT          843 a         1377 c         1320 g          813 t
ORIGIN      
        1 cacaatgcat ctcggcggcc cggaactgct catcatattg gtcatcgtcc tgctgatctt
       61 cggcgtgggg cgcatctccc gcgtcggtaa ggaattgggc accggcattc gcgagtttcg
      121 caagggcatc tccgaagagc agacggaatc cggcaaatcc gaagagcagc agaagtagtc
      181 gacctttgcg atagaagtgc aggcctccgg cggtcggctc gacggctggg ggccttctgt
      241 tctgtggctc tactggtttg ggcaggaaaa gtcacggcac acgataagtt gccacgagct
      301 tgccgctcgc gtcccgtaga taagcgacgg tccccgtagc tccccatatt gcgtcgctcg
      361 cgccccagta caggtcggac ggcgtgtcgg ttccggtcct ggtatgcaca ttgatctccg
      421 cgtcggggaa cagtgtcaca tccgggaagg tatagcgatt ggttcttccg tcggacagcg
      481 tccatcccgt gaggttgatg acctcgccca ggttcgtgag aatgaccgtc tctccgttga
      541 gcgagcctgg ctcttcgatc tcacgaatga cgacgagagc cggaccggaa gtcgccggcg
      601 tttttgcggc tgtcgttgca ggaggcgcga tctcagttga gccaccggcc ggaataatga
      661 tttcctgccc gaccgacaag atcgcatctt cggacagatc gttcgccgcg agcaagcctt
      721 ccagcgatac gtcgaagcga agcgacagat ccccgagcgt gtcacccgac tggatcgcgt
      781 aagtgaatgg cccacgcgcc gtaggcgtga gagcaacagt cggcttttcg agaggggtcg
      841 aggcgggcgc cgccagcacg ggcgtagcct gcgcgactga gaccgggctc ggggcagccg
      901 gacgccgcgc gtcccatgca gacagaatca acagtgtcac cgtggccgag ataatcacat
      961 tcatcgcgac aaagagcgct gcgcgcctcc agctcatgcc ttgacctcga cagtcggata
     1021 acctgatgat aacacaaaac gctccagttt tgcttggcgc atacacccga cccttactat
     1081 gacgggtggg aagtcggctg gtataatcgt cgcgcctgag tggcgctcat gaaacgaaca
     1141 ctcacccgca gacaactctt gaatcttgcg ggcgcttcgg ccatcgccgc gttcctggac
     1201 gcgtgcagcg cgccggcgcc ggaacaagcg actgcgacgc cttctgggtc caccggatat
     1261 cgcgcctatc tgccgaacgt catcgcagag accactccta cccctgctcc caagccgacg
     1321 gcgaagccgt cctcgcccga gccgacgccc gtgccgaggc ggacatttcc cgccttcgcc
     1381 cgaccgagca agctaggcat cgccgtgcag cgcttccgct atccgcagat catggatcgc
     1441 atcatcgccg acggacatcc gcgcgtcgtc aaaatcttcg aggatctggg ggcggcggcg
     1501 gagatcaagc ggcgctcgcc gaacacgatc gtcgtcgggc acatccacca ggcctatgat
     1561 ttccagaacg ccattacctc cggcgcgact gatatggccg cctacgccgc cgacttcgtc
     1621 gcccgacatt tgagccagta ccaggtcaac acgggcgtcg attactggga aggccacaac
     1681 gagccggtct tcgagaacga agcgaagatg gcgctgtacg gccagtttga agccgagcgc
     1741 gtccggcaga tgacggccta cggcttcaag tgcgccgtcg gcaacttctc tgccggcaac
     1801 ccgccgttcg agcaatggga tgagttcttt ccggcgctgc gagcaattcg agagtacggc
     1861 ggcgcgctgg cgctgcacga gtattccgcg ccgacgcttg actacggtta cgatccatct
     1921 tcagacgacg gttcgctgac cttgcgctat cgccgggttt accgtcaggt gatcccggcg
     1981 gacctgcatg tcccgattct catcaccgag gccggcatcg atggcttggt gagtgccgat
     2041 cggatcgggg caggttggca ggatttcatc tggtattgga ataagattcc gctcgacccc
     2101 gacgccttct gggcgtatat ggatcaactt caatggtatg acgaggaact gcaaaaagat
     2161 gattgggtca tcggcgcgac gctgttcgtg gcgggtgggc aagcccactt cgagtcgtac
     2221 gaactcgtcg gcgagatggg ggagctgctg acgcagtact ggatggcgca tcccgtgggc
     2281 tgatggctga tagctgatgg cgaatcgctt atcgcatatc gcttatcgtg cgtcacgcat
     2341 cgtctgtggc aatggatgac ctcgagcggt tctggtcgaa tattttttcg gaagatgcgg
     2401 cgcggatcgg cgaggcgtgg gcaacgctca gcgcgggcga gcgtgaggcc atcgccggac
     2461 acctgcgctc gatcgtgacc gattccgatc ggcctcccga gcagcgccat gcggcaagta
     2521 tcgccctatc cgtcgcgagt ggtaagtctg cgccgacgat cctgctggct tccggctccc
     2581 cgcgccgccg cgagttgatc gcactgttgg gtctgccttt cgggacgacg cccgcggacg
     2641 tcgacgagac gccgcattcg ggcgaggaac cggccgccat ggtcgttcgc ctcagcctca
     2701 ccaaagccga atctgcccga gcgaccacgc gaccatcgga ctacccgact acccgaccca
     2761 ttctcttggc cgccgacacg accgtctcgc tcgacggcga aatcctcgcc aagcccacca
     2821 ctgccgtcga agcgcgatcg atgctcacac ggctgcgcgg ccgttcccac caggtctaca
     2881 cggcgatcac gttgatcaac ttggagaccg gcgaacaaat caccgacctc gccacgacgg
     2941 aagtgccgat gcgggactac agcgacgccg aaatggaggc ctacatcgcc agcggcgatt
     3001 cgctggataa ggcaggaggc tatgcgattc agcacagtgg gttcaatccg gtggcgaaaa
     3061 tgagtggatg ctacgccaac gtcgtcggac tgccgttgtg ccacgtgaca cgctcactgc
     3121 gcgcactggg cgtcgagccg ccagttgacg tgccttcggc gtgccaggag catttggatt
     3181 acgagtgccc ggtgtttcag gcgatccttg ccaggcgtga aacgtgatgc gtaaaacgtg
     3241 atgaagtgat acgtgatgcg tgaatcattc gtcattctct ctcctctcca cttcacgcta
     3301 taatctcctc atgcccaagc gtctgctccc cttcatcgtc gccctcgcca ctgcatgcag
     3361 cgggccgaca tcgacgccaa ccatccggtt gtccactgcc acaccccttc ccccgcctcc
     3421 tgagcaaacc gcgctcgcct acttcgacgc ctggcagcgc gccgattacg ccggcatgta
     3481 cgaattgctg acaccgtcgg cgcaactggc cgtcaacgaa aaccagctcg ccagccgcta
     3541 cagccaggca catgcgacgg cgaccgtcaa gttcgtgcgc gcgacgctca agtccgccct
     3601 gcgggacggc agccgcgtca cggttgacta ccgtctcgaa tgggataccg ttctcttcga
     3661 aacactcatt accaattact ccctcactct cgtcgtcgat cctgtgcgta accagtgggg
     3721 cgtcgactgg aacgacggcc tgatctggcc ggcactcgaa ggcggcaaca tcctatcgct
     3781 caattacacc atcccgcgcc gcgcgaatat ctacgatgtg gacggcaagg ggctggctgt
     3841 cgagggcaag gtcgtcaccg tcggcgtcgt gccggggcaa gtcgccgacc gggcaacttt
     3901 gattcctcaa ctgagcgcga ttcttaacct gagcctggac ttcatcgaat cacagatcgc
     3961 cagtgcgcag ccggagtggt acgtgccact cgcaaacatc tcgttcgatg cgtttcaggc
     4021 gagccggtcg attctggaac agcccggcgt cgaactgcga gagaaggcag cgcgcgagta
     4081 tcgcggcggc ggggcgcacg ccatcggata tgtgggtttc atcagcccgg aggagatcga
     4141 ggcgtggaaa gcgcgtggtt atcagggcga cgagctcatc gggaaggcag gcctcgagca
     4201 gtggggcgag acgatcctgg ccgggacgca cggtgtgacg ctgaacgcga tcacgcccga
     4261 cggccagatc gtcaaggagg tcgcagtccg gccagccgcg cccagccgca gcatttacct
     4321 gacgatcaaa cgcgacttcc agcagcaagt cga
//
LOCUS       DBNHXB010000117         6054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_189569, whole genome shotgun sequence.
ACCESSION   DBNHXB010000117 DBNHXB010000000
VERSION     DBNHXB010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_189569"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="AC162G_04525"
     CDS             complement(<1..298)
                     /locus_tag="AC162G_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075112.1"
                     /translation="MISRRVWLILGAIVLAYVVTLAFDLTPYVRGPEEWRWNRYPAPH
                     WDRLWPIALALVAVVGWVEWINRRLSQSPHARRWTIIGVGALMLAALALQLLALR"
     gene            complement(295..2493)
                     /locus_tag="AC162G_04530"
     CDS             complement(295..2493)
                     /locus_tag="AC162G_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075113.1"
                     /translation="MSDPSIRAGVATRVWSPGVVWAQVSPLIPAGLAYILLVCLAYTP
                     IIASLSSAIPGTSQYEDFAIFHWNLWWFQHAIFRLGQDPFFTNYVFFPHTINLAYHTF
                     TPFLDIIGLPVYAALGATAAINVLIVGSLVFNGLAMFAFLHHHKVPTGLAFVGGALFA
                     FTPFTTARVSFVHLNTLPIGWLPLGLLTWDRLVERQTLGAALVLSGVLYAAFMTDQHF
                     GIWLALLLTPYALFRLVRSGARTRRRTVLLGALAAIVLIGFMLIAPLPQVLAGRNVEY
                     PTTPLPHPVLQSRYSLHLDDLIAWPPRYLNSERVTLGVLLPVGAAIGLVRGRRAAGRV
                     FWLVMGAAFLVLAFGPTFEPLNLPLPYQLFHRLTGGLYRVPARFIILTVFALIVFAVT
                     SLRSDYQRLGRATRWACVVGALLWLAVENRWYEPFPTFHMPDYRIYHAIGADPDVYLL
                     LEVPVGPHNTGDRIFGHGGVLQYFAWIHHKRLINGAISRVSTDLTEWYRQWPLITALA
                     EEGPLPDLPLARREFQHLSDEWDIRYVLLHRDMLTPDVATWAAGLFNTQAGWCLVDEE
                     GALLAYRRTDTPCRLSERLRLPPASRDTLGLGDGSDERYLGPGWYYAEDIGGPQARWT
                     GREPATTLRVALVRRDYRVSLRAASYVPDQIVTVYANGRRVADLSIGEGWGEYTFDLP
                     AESISADGLLTLTFVHAHAWSPHERTGGQSDDRRPLAVAYDSITFTPEVP"
     gene            complement(2508..3851)
                     /locus_tag="AC162G_04535"
     CDS             complement(2508..3851)
                     /locus_tag="AC162G_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075114.1"
                     /translation="MTGSAIDRPSIAQLFVLRLDGLFAHRLVGAVLFMAFLVLWATLA
                     KAAVTRSWQEQSRMATVQTLVEQGTFIIDHTLFNNTGDKIFVNGHFYSDKTPLLSVAA
                     AGLYSVLYNVFHLTLDPIDCIPDDDPAACRAFGPGGPRFTAFYWLNLAFMSLPSALLV
                     VLFWKALIDQQVGGLAAVGLAVLLGIASPIAPYSIVFVGHVPAALFLFAGFMLLARPT
                     ASGRAFFWAGLFVSLAANIDLLLALFVVAFGAWISLTRRSHVLPFAVGALIPFAMTAA
                     INYWAAGTIVPLYLDPKAYDFPGTVLNKTVGGTDGFYSLEFGLNYAYEMLAGRRGVFA
                     FTPLLVCAVAGLWLAARDRSHRLRSPAIAVIAASLAFTAYLILRTDNFGGVAWGTRWF
                     VPLIPVLWTFIPLVFTRSHSRAWKAVLLATAIVSLRTALPGLHDPWGEYQPIFRL"
     gene            complement(3851..5884)
                     /locus_tag="AC162G_04540"
     CDS             complement(3851..5884)
                     /locus_tag="AC162G_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075115.1"
                     /translation="MRSVPKRWLENRWLGLAIVFAIGVELRTIRLADVPGGIFHDEAW
                     SAVKAMALLNGETPPQVYFAENNGMDAAHVYGVAALFALTGPLAIGSRLVSSLMGSLT
                     VLATYWAAWELFAEQRHRHGLSLISAFVVATLFWALTTSRSAWHVPWMTLATTLSLAA
                     LFRGRRLVHRRWFVIAGLLAGLAQYTYPSARFLPALLLLLAALDLWDNRPARRTVILN
                     YLSLVAAAAIVFAPLGVYFAQNPEWFFVRAQQTADAASLDLGHNLIQTLAGFSIYGDS
                     EGLHNLNGRPALDAILTIFFIAGLIVCLAKRRAAHPSTAPSPSPRSGSGLRLRAGLIL
                     IAWLIVFSLPVVLTGQAPLFRRWTGILPAQAMLAALGAVALADLVRERIASRAGRASR
                     LVPIAVAASLMISAALSVADYFGPYASAPTMFWAYDRGITQVAEYIASRPDTTVFLTP
                     YDRFYEVVAITLAEAGRAPIQSYNGRACAVFPETTSRETEWVVITEQDDRTLPLAQQL
                     FPAGEIVWQIDSPIGAYARAWHVSAGQTARLKLDQRGRADLGGKVRLIGYELPESIRP
                     GETLRVAVALEATSPLEQRYKVFVHLRGEGDSVLAQADRLPCNFSLNEADWRPGDIVL
                     EEYTLPISPETAPGTYRVILGVYRPDTHARLPVLSSDPPHTGDGVVLGEIDVK"
BASE COUNT         1330 a         1854 c         1973 g          897 t
ORIGIN      
        1 ctcgtaaggc gagcagttgc agtgccagtg cggccagcat cagcgcgcca acgccgatga
       61 tcgtccagcg gcgcgcatgg ggcgactgtg acagacggcg attgatccat tcgacccaac
      121 cgaccacggc gacgagtgcg agcgcgatgg gccacagtcg atcccagtgc ggcgcaggat
      181 agcgattcca gcgccattcc tcaggtccgc gcacgtaggg cgtgagatcg aaggccagcg
      241 tgacgacgta cgccaacacg atagcgccca ggatgagcca cacgcgacgc gagatcatgg
      301 aacctcgggt gtgaaggtta ttgaatcgta cgccacggcc aggggacggc gatcgtcgct
      361 ctggccgccg gtacgctcgt gaggcgacca ggcgtgcgcg tgcacgaacg tcaaggtcag
      421 gaggccgtca gccgagattg actcggcggg caggtcgaaa gtgtattctc cccagccttc
      481 accgattgac aggtcggcta cccggcggcc attggcatac acggtcacga tctgatcggg
      541 cacgtaagac gcggcgcgca gagagacgcg atagtcgcgg cgcacgagag ccacgcgcag
      601 cgttgtcgcc ggttcgcgtc cggtccatcg cgcctgcggc ccgccaatgt cttcggcgta
      661 ataccagccc ggacccaggt agcgctcgtc gctgccgtcg cccaggccta gcgtgtcgcg
      721 cgaagcgggc ggcagtcgca ggcgctcgga taggcggcac ggggtatcgg tgcggcggta
      781 agccagcagc gccccttcct cgtcgaccag gcaccagccc gcctgggtgt tgaacaatcc
      841 tgccgcccac gtcgccacgt ccggcgtgag catgtctcgg tgcagcagca cgtagcggat
      901 gtcccactcg tcgctcaagt gttggaactc ccgtcgcgcc agaggcaggt cgggaagcgg
      961 cccttcctcc gccaatgccg tgatcaaagg ccattgtcga taccattcgg tcaggtcagt
     1021 cgagacgcgc gatatggccc cattgatcag gcgcttgtgg tgaatccagg cgaaatactg
     1081 taggacaccg ccgtgaccga atatgcgatc ccccgtgtta tgcggtccga cggggacttc
     1141 caggagcaag tacacatcgg ggtcggcgcc gatggcatgg tagatgcggt agtccggcat
     1201 gtgaaaggtg gggaacggct cgtaccagcg attctccacg gccagccaca gcagcgcgcc
     1261 caccacgcac gcccagcgtg ttgcacgccc cagccgttga tagtctgacc gcagcgacgt
     1321 caccgcgaac acaatcagcg cgaacaccgt caagatgatg aaacgagccg gcacgcggta
     1381 caggccgcca gtcaggcggt gaaagagctg atacggcaac ggcagattga gcggctcgaa
     1441 ggtcgggcca aaggccagca ccagaaaagc cgcgcccatg accaaccaga agacccgccc
     1501 ggctgcacgc ctgccgcgca ccaagccgat tgctgcaccc accggcaata gcacgcccaa
     1561 cgtcacgcgc tcgctgttca aatagcgcgg gggccaggcg atcagatcat ccaaatggag
     1621 cgagtaacga gactgaagga ccggatgagg aaggggtgtc gtcggatatt cgacattgcg
     1681 cccggcgagc acctgcggca acggcgcgat cagcatgaag ccaatcaaga caatcgctgc
     1741 gagcgcaccg agcagaactg tgcgccgccg cgtgcgcgcg ccgctgcgca ccaggcgaaa
     1801 cagcgcatac ggcgtgagca agagagccag ccatatccca aagtgctggt cggtcatgaa
     1861 ggcagcgtag aggactccgc tcaatacgag ggccgctccg agcgtttggc gctcgacaag
     1921 acgatcccag gtcagcaatc ccagcggcaa ccagccgatt ggcagggtat tcaagtggac
     1981 gaacgaaacg cgtgccgtgg tgaacggcgt gaaggcgaac agcgcgccgc cgacgaaagc
     2041 cagaccggtc ggcactttgt gatggtgcaa gaaagcaaac atggccagac cattgaacac
     2101 cagtgaaccg acgatcagaa cgttgatcgc agcggtcgca ccgagcgccg cgtaaaccgg
     2161 gaggccgatg atatcgagaa aaggcgtgaa cgtgtgatag gccaggttaa tagtatgagg
     2221 gaagaaaacg tagttcgtga agaacgggtc ttgtcccagg cggaagatgg cgtgctgaaa
     2281 ccaccacaag ttccagtgga agattgcgaa atcttcgtat tggctggtgc cagggatagc
     2341 cgacgagaga cttgctatga tgggagtgta tgcgagacaa accaggagaa tgtaggccag
     2401 cccggccggg ataagcggag acacttgtgc ccacaccacg ccgggcgacc acactctggt
     2461 ggccaccccg gcgcgaatcg agggatcaga cattcgtgct cctcatatca gaggcgaaag
     2521 atgggctggt attcgcccca tggatcgtgc aggcccggca gcgcggtccg aagcgaaacg
     2581 atggccgtgg cgagtagtac cgctttccat gccctgctgt gagagcgcgt gaacacgagc
     2641 ggaatgaagg tccacagcac aggtatcagc ggcacgaacc agcgcgttcc ccaggccacg
     2701 ccgccgaagt tatccgtgcg cagaatcagg taagctgtga aagcgaggct ggcagcgatc
     2761 acggcgatcg ccggtgagcg cagccggtgc gagcgatcgc gagcggcgag ccacagccct
     2821 gccactgcgc agaccaagag gggtgtgaag gcaaacacgc cgcgcctgcc ggcgagcatt
     2881 tcgtaggcgt aattcaagcc gaattcgagc gaataaaaac cgtctgtgcc gccgaccgtc
     2941 ttgttgagaa ccgtgccggg aaagtcgtaa gccttgggat cgagatacag cgggacgatg
     3001 gtgccggcag cccaatagtt gatcgcggcg gtcatggcga acgggatgag ggcgccaacg
     3061 gcaaacggca gcacgtgcga gcgacgtgtc aatgagatcc atgcgccgaa tgcaacgacg
     3121 aacagcgcca acaggaggtc gatgttagcg gccagactga cgaacagtcc cgcccagaaa
     3181 aatgcgcggc cagacgcggt ggggcgagct aagagcataa acccggcgaa caggaaaagt
     3241 gccgccggaa catgtccgac aaacacgatc gaatagggcg cgatcggcga tgcgatcccg
     3301 agcaagaccg ccagtccgac tgctgcgagg ccgcccactt gctgatcgat caacgccttc
     3361 cagaacaaca ccaccaacag tgcggatggc aggctcatga atgccagatt cagccagtag
     3421 aacgccgtga agcgcggccc gcctggcccg aacgccctgc aggctgccgg atcgtcgtcc
     3481 gggatacagt cgatcggatc gagcgtgaga tggaagacgt tgtacagcac cgaatacagg
     3541 cctgccgctg ctaccgacag gagcggcgtc ttgtcggagt agaagtgccc gttgacaaag
     3601 atcttgtcgc cggtgttgtt gaagagggtg tggtcgatga tgaacgtgcc ctgttccacc
     3661 agcgtctgca ccgtcgccat gcgcgactgt tcctgccacg agcgggtgac cgccgccttg
     3721 gcaagcgtcg cccacaacac cagaaaagcc atgaagagta cagcgcctac cagacggtgg
     3781 gcgaacagcc cgtcgaggcg aaggacgaag agctgagcga tagatggccg atcgatcgcg
     3841 gagcctgtca ttacttcacg tctatctcgc ccaacacaac accgtcgccg gtatgcggtg
     3901 gatccgacga cagtacgggt aagcgagcat gagtatccgg tcggtaaacg ccaagtataa
     3961 cacggtacgt acctggcgcg gtctccggcg agatgggcag agtgtactcc tcgagcacaa
     4021 tgtcacccgg tcgccagtcc gcttcgttca gcgagaagtt gcacggcagc cggtcggcct
     4081 gcgccagcac cgaatcaccc tcgccgcgca aatgaacgaa cactttgtag cgctgctcaa
     4141 ggggtgatgt ggcttcgagc gcgacggcaa cccgcagtgt ttctcccggg cgaatgctct
     4201 ccggcagctc gtacccgatg agtcgaacct tgccgccgag atcggcgcgg ccgcgttggt
     4261 caagcttcag tcgagcggtc tggccggccg agacgtgcca ggcgcgcgca tacgccccga
     4321 tcggcgaatc gatctgccag acgatctcgc ctgccggaaa caactgctga gccagcggca
     4381 gcgtgcgatc gtcctgttcc gtgatcacca cccactcggt ctcgcgcgaa gtggtctctg
     4441 gaaacaccgc acacgccctg ccattataac tttgaatcgg cgcgcggccg gcctccgcca
     4501 gcgtgatggc gacgacttcg tagaagcggt cgtacggcgt gaggaaaacg gtcgtatcgg
     4561 gccggctggc gatgtactcg gcgacttgcg tgatgccgcg atcgtacgcc cagaacatgg
     4621 tcggtgcgga cgcatatggc ccgaagtaat cggcgaccga cagcgcagcg ctgatcatca
     4681 gagaggcagc cacggcgatc gggacgagcc gcgatgcgcg acctgcacgt gacgcgatgc
     4741 gctcgcgcac gaggtccgcc agcgccaccg cgcccaaggc agcgagcatc gcctgggcag
     4801 gcaggatacc cgtccagcgc cggaagaggg gcgcttgccc ggtcagcacc accggcagcg
     4861 aaaagacgat gagccaggcg atcagaatca ggcctgccct gagccgaagc cctgaccctg
     4921 agcgcggcga aggggaaggg gcagtcgaag ggtgagccgc gcggcgcttc gccaggcaca
     4981 cgataagccc tgcgatgaag aaaatcgtca ggatcgcgtc gagcgcaggc cggccgttaa
     5041 ggttgtgcag tccctcgctg tcgccgtaga tcgagaatcc ggccagcgtc tggatgaggt
     5101 tgtgcccgag gtcaagcgat gcggcatcgg ccgtctgctg agcgcggacg aaaaaccatt
     5161 cgggattctg agcaaagtac acgccgagcg gcgcgaagac aatcgccgcc gcagcgacta
     5221 aactgaggta attgaggatg acggtgcgcc gcgccgggcg gttgtcccac aggtcgagcg
     5281 cggcaagcag caagagcagc gccggtaaga atcgcgccga aggatacgtg tactgtgcga
     5341 gtccggccag caggccggcg atcacgaacc agcgccggtg caccaggcgg cgcccacgga
     5401 acagggcggc caggctcagc gtggtcgcca gcgtcatcca cggaacgtgc caggctgacc
     5461 ggctggtggt caatgcccag aacagcgtgg cgacgacgaa ggcagagatc aaggatagcc
     5521 cgtgtcgatg ccgctgctcg gcgaataact cccaggccgc ccaatacgtc gcgaggacgg
     5581 tcaggcttcc catcagcgac gacacgaggc ggctgccgat cgccagcggg ccggtcagcg
     5641 cgaacagcgc ggcgacgccg tacacatgcg ccgcgtccat gccgttgttt tcggcgaagt
     5701 acacctgcgg cggcgtttcc ccattgagga gcgccatggc cttaaccgcc gaccatgcct
     5761 cgtcgtgaaa gattccgccc gggacgtcgg cgaggcgaat cgtgcgcagc tcgacgccga
     5821 tcgcgaacac gatggcgaga cccagccatc gattttcaag ccagcgcttg ggaacgctcc
     5881 tcatgaatca acgctcgacc tgaatctggg tcagatagat gcgatctcct acgcgccggt
     5941 cgccgacgaa gatcggcagg cgctcgccgc tgtcccgacg atagactccc gtcccgatcc
     6001 agtacgagcc agccggtgta tccggcggca catggataaa gtcgtgtacc tgga
//
LOCUS       DBNHXB010000118         3716 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2035585, whole genome shotgun sequence.
ACCESSION   DBNHXB010000118 DBNHXB010000000
VERSION     DBNHXB010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2035585"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="AC162G_04545"
     CDS             complement(<1..214)
                     /locus_tag="AC162G_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075116.1"
                     /translation="MSGALWAIVAGVGFGVFQSLNRRAVSGMDVYLATFLQLLISALV
                     LAAISLLTVDVGLVSRAPMEALISFAL"
     gene            complement(226..951)
                     /locus_tag="AC162G_04550"
     CDS             complement(226..951)
                     /locus_tag="AC162G_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5075117.1"
                     /translation="MLLELREVEAGYLPGISVLQGVSLHVDPGELVCLIGPNGAGKST
                     VLRAISGLLRPTQGCIQFDGEDIGGMRPDLVLGHGIAHVPQGHSTFPAMSVHENLLMG
                     AYTLRDRAERQRRLDRVYAMFPFLAERRKDKAGNLSGGQQKILEIGRALMMSPRMMLL
                     DEPSLGLAPKMARQVFETISRLKDEAGMTILMVEQNAKSGLAISDRAYVLELGKERLA
                     GPAGTLLNDPRVAQLYLGGTLAS"
     gene            complement(1089..1823)
                     /locus_tag="AC162G_04555"
     CDS             complement(1089..1823)
                     /locus_tag="AC162G_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5075118.1"
                     /translation="MTALLQAEKVTRRFGGIVAVNECSIAVQERSITGLIGPNGSGKT
                     TLFNLITGVYRPHSGHVYLRGRRITGLQPSRICHLGLSRTFQITRLFWDLTVLENMIV
                     PVRQVGFRPLFGRGMYQHEQERALALLDRVGLTHLKDQPARKLSFGQQKLLEFAAILM
                     AEPELVLLDEPAGGVNPTMIRFIMDLIRSLNRDGVTFLVVEHNMGVVMDLCDHILVMD
                     QGQKIAEGPPHIIQSDPVVLDAYLGT"
     gene            complement(1820..2881)
                     /locus_tag="AC162G_04560"
     CDS             complement(1820..2881)
                     /locus_tag="AC162G_04560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5075119.1"
                     /translation="MRRNVLRWWPLLAFVVLLAYPWLLREPYFHLLGFNVLLSATMAT
                     SWNIMGGYTGYKSLGHGAFFGLGAYLVAIAANQWGWNPLWSAPLLAIAVMLVAMALGW
                     IMLRTSGSAFVIATIAMLLIFRLLALNLGEITKGARGLSQPLPPWPPEFSRMPFFYYM
                     LVWLIVALVISAYVRRSRFGLGLLAIRDDEGKAEMVGVNTTLYKVIAFGLSAYFVAVG
                     GGIWSYVQTHISPVFAFGILVGVEMILMTMLGGLGTLWGPVLGAFILIPASDLILFTF
                     KSQIHLAILGALMLTVILFLPRGILPTLQDWLERRRAPRPAFAGARAMADVIAEQAHA
                     AGGVQPVPDELAVPEEAKR"
     gene            complement(2959..>3716)
                     /locus_tag="AC162G_04565"
     CDS             complement(2959..>3716)
                     /locus_tag="AC162G_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5075120.1"
                     /translation="LVFSAYIAYWLFTLYGLDPILSILITIPLFFLIGAVVQRFMLSR
                     VRSEPGLFVLVTFALAITLEGIMGALWKSTGRSVRTAYTGEVIKLAVLDFNLRLPVVR
                     IVGFGAAIAVLGFFHTLLLRSNLGRAIRATIQNATAAQLVGVNVSRVQAVAFGLGLAA
                     VAAGGSIFSLIWAFNAASHQEWISKMLSIIVLGGMGSLPGAFIAALIMGVVESVAAVT
                     MTTYLSPIVFYLVLFLVLILRPQGLMGTRIREG"
BASE COUNT          778 a         1226 c         1103 g          609 t
ORIGIN      
        1 ccagcgcgaa actgatcagc gcctccatcg gcgcacggct caccaggccc acgtccaccg
       61 tgaggagact gattgccgcc agcaccagcg cgctgatcag gagctgcagg aacgtcgcca
      121 ggtacacatc catgccgctg acggcgcgcc ggttgaggct ttgaaacacg ccgaagccca
      181 cgcccgccac gatagcccat aatgctccgc tcatgcgtca acagcctagc tcgccagcgt
      241 cccgccgagg tacagttggg ccacgcgcgg atcgttgagc aatgtgccgg ctggtcccgc
      301 cagccgttct ttgcctagtt caagcacgta agcgcgatcg gagatggcta gcccgctctt
      361 ggcgttttgc tcgaccatca ggatcgtcat gccggcttcg tccttgaggc ggctgatcgt
      421 ctcaaacacc tggcgggcca tcttgggcgc caggccgagc gatggctcgt cgagcagcat
      481 catgcgcggg gacatcatca gcgcgcggcc gatttccagg attttctgtt ggccgccgct
      541 caaattgccg gccttgtctt tgcgacgctc tgccagaaac ggaaacatgg catacacccg
      601 atccaaccgg cgctggcgct cggcgcgatc gcgcagcgtg tacgcgccca tcagcaggtt
      661 ctcgtgaacg ctcatcgccg ggaaagtgct atgcccctgc ggcacgtgcg cgatcccgtg
      721 tcccaaaacc aggtccggcc tcatcccgcc aatatcctcg ccgtcgaatt gaatacaacc
      781 ttgagtgggc ctcaacagtc cggagatggc gcgcagcacc gtcgacttgc ctgcgccgtt
      841 cggcccgatc aggcacacca gctcaccggg gtccacgtgc aggctgacgc cctgcagcac
      901 gctgattccc ggcaggtaac cggcttcgac ttctctcaat tcaagcagca tccccgagtt
      961 cctgtcacac ggatttccca cggatgacac ggatgggcaa gttcccctga ggtcccctca
     1021 gttccttgat cgtgcaggag actgcttcgt cgcacacaac gctcctcgca gtgacggctg
     1081 aacagttact acgtccccaa ataagcatcc agcacgaccg ggtcggattg gatgatgtgc
     1141 ggcggacctt cggcgatctt ctggccctga tccatcacca ggatgtgatc gcacagatcc
     1201 atcaccacac ccatgttgtg ctcgacgacg aggaacgtga cgccgtcccg gttgaggctg
     1261 cgaatgagat ccatgatgaa gcggatcatg gtcgggttga ctccgccggc cggctcgtcg
     1321 agcaacacca gttctggctc tgccatcagg atcgcggcaa actcgagcag cttctgctgg
     1381 ccgaacgaca gcttgcgcgc cggctggtcc ttcaagtgag tcaggcccac ccgatccagc
     1441 aatgccagcg ctcgctcctg ctcatgctga tacataccac gcccgaacag tgggcggaag
     1501 cccacctggc gcacgggcac aatcatattc tccagaacgg tcaggtccca gaacaggcgg
     1561 gtgatctgga aggtccggct cagccccagg tgacagattc gactgggctg caagccggtg
     1621 atacgccgac cgcgcaagta cacgtggccc gaatggggcc gataaacgcc cgtgatcagg
     1681 ttgaacagcg tcgtcttgcc ggagccgttc ggcccgatga ggccggtgat actcctttcc
     1741 tgaacggcga tcgaacactc attcacggcg acgatgccgc cgaatcgccg ggtgaccttt
     1801 tccgcctgaa gaagagccgt catcgcttcg cctcttccgg gacggccagc tcgtccggca
     1861 caggctgaac gccgccggct gcgtgggcct gctcggcgat cacgtcggcc atggcgcgcg
     1921 caccggcgaa agccggtcgc ggggcgcggc ggcgctccag ccagtcttga agcgtcggca
     1981 ggatgccgcg cggcaggaac aggatgacag tcagcatcag cgcccccaag atggccaggt
     2041 gaatttgcga cttgaaggtg aagaggatca ggtccgacgc cgggatcagg atgaacgcgc
     2101 ccagcaccgg cccccacagc gtgcccagcc cgccgagcat cgtcatcagg atcatctcca
     2161 cgccaacgag aatgccgaag gcaaacaccg ggctgatgtg cgtctggacg tatgaccaga
     2221 tgccaccgcc caccgcgacg aagtaggcgc tcagtccgaa ggcgatcact ttgtacaagg
     2281 tggtgttcac gcccaccatc tcggccttgc cctcgtcgtc gcggatggcg agcagcccta
     2341 acccaaagcg cgagcggcgt acgtaggccg agatcaccag cgcgacgatc agccagacga
     2401 gcatgtaata gaagaagggc atgcgcgaaa actccggcgg ccagggcggc agtggctggc
     2461 tgaggccgcg cgcgcctttg gtaatctcac ccaggttcag cgcgagcagg cggaaaatca
     2521 acagcatcgc aatcgtcgcg atgacgaacg ccgagccgct ggtgcggagc atgatccagc
     2581 ccagcgccat cgcgaccagc atcaccgcga tcgccagcag cggcgcgctc cacagcggat
     2641 tccagcccca ctggttggcc gcaatggcga ccaggtacgc gcccaaaccg aagaacgcgc
     2701 catgccccag cgatttgtaa ccggtgtagc cgcccatgat gttccagctc gtcgccatcg
     2761 tggcagacag cagcacgttg aaccccagga gatgaaagta gggttcacgc agaagccagg
     2821 gataggccag caacaccacg aaggccagca gcggccacca gcggagcacg tttcgacgca
     2881 tcgttcagcc ttccctgata cgcgtgccca tcatcttgga ggttgggatt tgaagtttgg
     2941 ggtttgaagt tgcgccattc agccttcccg tatgcgcgtg cccatcaacc cctgcggccg
     3001 gaggatgagg acaaggaaca aaaccaggta gaagacgatg ggtgacagat aggtggtcat
     3061 ggtgaccgcc gccaccgatt cgacgacgcc cataatcagc gccgcgatga acgcacccgg
     3121 caggctgccc atacccccca atacgatgat ggataacatc ttagaaatcc attcctggtg
     3181 gctggctgcg ttgaacgccc agatcaggct gaagatcgac ccgcctgcag ccaccgccgc
     3241 taaccccagt ccaaaggcca ccgcctgcac gcggctcacg ttaacaccca ccagttgcgc
     3301 cgccgtcgcg ttctggatcg tcgcgcgaat ggcccgtccc aggttgctcc gcagcaaaag
     3361 cgtgtgaaag aaaccgagca cggctatcgc cgccccaaac ccgacgatgc gcactaccgg
     3421 caggcgcagg ttgaaatcca gcacagccag cttgatcacc tcgccggtgt acgccgtgcg
     3481 caccgagcgg ccggtggact tccacagcgc gcccatgatg ccctccagcg tgatggcgag
     3541 tgcgaatgtc accagcacga acaggcccgg ctcgctccgc acgcgggaca gcatgaagcg
     3601 ctgcacgacg gctccgataa ggaaaaacaa cgggatagtg atcaggatcg ataggatcgg
     3661 atcgaggccg tacagcgtga acagccagta ggcgatgtag gcgctgaaca ccagga
//
LOCUS       DBNHXB010000119         3384 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3713495, whole genome shotgun sequence.
ACCESSION   DBNHXB010000119 DBNHXB010000000
VERSION     DBNHXB010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3713495"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="AC162G_04570"
     CDS             complement(<1..191)
                     /locus_tag="AC162G_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019421718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5075121.1"
                     /translation="MITHALTVGPVQTNCYVVVCETTRQAVVIDPGWNASSILAAVDS
                     FQAVVKLILLTHAHFDHIGA"
     gene            complement(188..445)
                     /locus_tag="AC162G_04575"
     CDS             complement(188..445)
                     /locus_tag="AC162G_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075122.1"
                     /translation="MDDRDKLTAILDKLNSDDIRLLARFAQIMIEEDKSDPIHDPTDL
                     RERYAQFKKWCESNDLDLAGVDAWVEETRQTDDGAADPRSS"
     gene            complement(669..1103)
                     /locus_tag="AC162G_04580"
     CDS             complement(669..1103)
                     /locus_tag="AC162G_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5075123.1"
                     /translation="MKTVSMLLESKGNDVWSVSPGTTVYDALALMAEKNVGALLVLES
                     GRLVGIFSERDYARKVILYGKSSRQTTVGEIMTPKVVYVRPEQTIEACMALMTDKRIR
                     HLPVLEGDRVIGVISIGDVVKAIISEQEFMIEQLESYIAGTR"
     gene            complement(1276..1776)
                     /locus_tag="AC162G_04585"
     CDS             complement(1276..1776)
                     /locus_tag="AC162G_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075124.1"
                     /translation="MSPSRWASAAAGVAVITLAISFLSSRAPALGALPVLLGLLWIAA
                     AGQRWEWIAGPMWIGCVGLAALATFVGAHPIGPPVGALAALAAWDLDGLHRRLRSATR
                     TYDESRIVERHSRRLAGVLGLSLALSLVTLVARVRLDLCWAMALGLLAALCLSRAVRF
                     MRRESG"
     gene            complement(1776..3068)
                     /locus_tag="AC162G_04590"
     CDS             complement(1776..3068)
                     /locus_tag="AC162G_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR5075125.1"
                     /translation="MSQRSALLIGVILTLLLLGLGARSAGILVLILPPAIYLAAGLLF
                     GPDALRLEATRTLGGDYAAPDEPVTVSVTVTNRGSRLEIVMLEDRVAPPATPADGGSA
                     PQVAALEPGETIEIAYTLRMGRGCYRFQGVQATASDTFGVFQREALAPAPGQFFVLPN
                     VLRLRRIAIRPSRTRVYAGDVPARQGGPGIDFFGVREYQSGDPLHWINWKATARAWEA
                     IFTNEFEQERIADVGLILDVRRRAYSGPNGHPIFEHAVLAAAALAEAFLKAGNRVGVL
                     LYGMLLDWTFPGYGKRQRRRVLHALARAAPGDSMVFDRLEYIPTRLFPPQSQLVVISP
                     LLEDDVEPLIALRAHNYSLMVISPDPIPVELEALGRRPSAALAARLARIERALVIRKL
                     RQAGARVLDWNVGVPFEEAVYAAFGRAPPQARPVRTWL"
     gene            complement(3068..>3384)
                     /locus_tag="AC162G_04595"
     CDS             complement(3068..>3384)
                     /locus_tag="AC162G_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5075126.1"
                     /translation="VETVHVVPDVERYIVALVNETRADRRVAVGASPRGSLALLTLSR
                     AWAALDGRDYVLPDDVKLFAEPALAHRLVLRPDLWMKQKAAEEVIASVLQSVPVPVVE
                     GA"
BASE COUNT          642 a         1141 c         1078 g          523 t
ORIGIN      
        1 gcgccgatgt gatcgaagtg cgcgtgcgtg agcaggatca acttgaccac agcctggaag
       61 gaatcgacgg cggcgagaat cgaggaagcg ttccagcccg gatcgatgac gacggcctgg
      121 cgcgtggttt cacagacgac gacgtagcag ttggtctgaa ccggcccgac ggtgagcgcg
      181 tgggtgatca tgacgagcgc gggtcggccg cgccgtcgtc ggtctggcgc gtttcttcaa
      241 cccaggcatc cacccccgcc aagtcgagat cgttcgactc gcaccacttc ttgaactgcg
      301 cgtaacgctc gcggaggtcg gtggggtcgt gtatcggatc cgacttgtct tcctcgatca
      361 tgatctgcgc gaagcgcgcc agcaggcgga tgtcgtcgct gttcagtttg tccaggatcg
      421 ccgtcagctt gtctcgatcg tccatcattc ccctcttgat cgtctcccgc cgcgctctgt
      481 ttgtggcaag cgcccatgaa aaccccgatc cgctctcggc ggaatgccaa atccgtcgct
      541 ggatttgaca atccagcggg agcattcacc cggggtttga aaggcgctca gcataaccac
      601 agagagcgca gacgtcgcgg aggtcttgga atccccgtcg aacggattat aacatagaga
      661 aacacggcct atcgggttcc ggcgatgtag ctctcgagct gctcgatcat gaactcctgc
      721 tcggagataa tcgccttgac gacgtcgcca atcgagatga cgccgatcac gcggtcgcct
      781 tcgagcacgg gcaggtgccg gatgcgcttg tcggtcatca gcgccatgca cgcctcgatc
      841 gtctgctcgg ggcgaacgta gacgaccttc ggcgtcatga tttcgccgac ggtcgtctgc
      901 ctggacgact tgccgtatag gatcactttg cgcgcgtagt cgcgctcgga gaagatgccg
      961 acgaggcgac cactctccag cacgagcaac gcgccgacgt tcttctcggc catcagcgcc
     1021 agcgcatcgt aaaccgtggt gcccggcgac accgaccaga catcgttacc cttggactcc
     1081 agaagcatgc tcacggtctt catgtctcga ctcctccttc ggcagcaggc taaggacgta
     1141 cgaaagaatc actccgcact ttctccgctc ccaccggatt tggcaatccg gtgggagcgg
     1201 ctggatgccg atgattatgg cgcaagcttg ccctgagcga aagcgaaggg tcagcccgtc
     1261 aaggcaaacg cgatctcagc cgctctcgcg ccgcatgaag cgcacggccc ggctcagaca
     1321 caaggcagcg agcagtccca gcgccatcgc ccagcagaga tcgaggcgaa cacgcgccac
     1381 aagcgtgaca aggctcagcg ccaggctcaa ccccagaaca ccggccagac gccggctgtg
     1441 ccgctccacg atccggcttt cgtcgtaggt gcgcgtcgcg ctcctcaacc gccggtgcag
     1501 gccgtcgagg tcccaggccg ccaacgccgc aagcgccccc acaggcggcc cgatcgggtg
     1561 cgcgcccacg aatgtggcga gtgccgccag cccgacgcag ccgatccaca tcggcccagc
     1621 gatccactcc caccgctgcc cggcggcggc gatccacagc aggccgagca ggaccggcag
     1681 ggctccgaga gccggagcac gactggagag aaaactgatc gccagcgtga tcaccgccac
     1741 gccggcagca gcactggccc atctcgacgg tgacatcaca gccaggtcct cacagggcgc
     1801 gcctgaggcg gcgcgcgacc gaacgcggcg tatacggcct cctcgaatgg gacgccgacg
     1861 ttccagtcca atacgcgagc gccggcctgg cgcagcttgc ggatcacgag cgcacgctcg
     1921 atgcgcgcga ggcgggcagc cagcgccgca gacgggcgcc tgccgagcgc ctcgagctcg
     1981 accgggatcg gatcagggct gatgaccatc agcgagtagt tatgggcgcg aagcgcgatg
     2041 agcggctcga cgtcgtcctc gagcaggggg ctgatcacga ccaactgcga ctgaggtgga
     2101 aacaggcgcg tgggaatgta ttccagtcga tcgaagacca tgctgtcgcc cggcgcagcg
     2161 cgggccaggg cgtgcagcac acggcggcgc tgccgcttac cgtagcctgg aaatgtccag
     2221 tcgagcagca tgccatacag caggacgccg acgcggttgc ccgccttcag gaaagcttcg
     2281 gccagcgccg ccgccgccag gacggcgtgc tcgaaaatgg gatggccgtt tggcccggaa
     2341 taggcgcgcc tgcgcacgtc gagaatcagg cccacatcgg cgatacgctc ttgctcgaat
     2401 tcattcgtga agatcgcctc ccaggcacga gctgtcgctt tccaattgat ccaatgcaag
     2461 gggtcgccgg actggtactc acgcacgccg aagaaatcga tgccaggacc gccttgccgc
     2521 gccgggacgt cgcccgcgta cacgcgggta cggcttggtc gtatcgcgat gcggcgcagg
     2581 cgcagcacgt tcgggaggac gaagaactgg ccgggcgcag gcgccagtgc ttcgcgctga
     2641 aacaccccaa aggtatcgct cgccgtggcc tgcacgccct gaaaccggta gcagccccga
     2701 cccatccgga gcgtgtaggc aatctcgatc gtctcgcccg gctccagcgc agccacctgt
     2761 ggcgcggacc cgccatcggc tggggtcgcc ggcggagcga cgcgatcttc cagcatcaca
     2821 atctccaggc gcgatccccg gttcgtcacg gtcaccgata ccgtgaccgg ctcgtccggc
     2881 gcggcgtaat cgccgccgag cgtgcgggtc gcctcgaggc gcagcgcatc cggcccgaac
     2941 aggagacccg cagccagata gatcgcaggc ggcagtatca gcacgagaat gcccgcgctg
     3001 cgcgccccca gtcccagcag gagcagcgtc aggatcacgc cgatcaagag cgccgaccgc
     3061 tgcgacatca cgctccttcg acgaccggaa cgggcaccga ctgaaggacg gacgcgatca
     3121 cctcctcggc cgctttttgc ttcatccaca agtccggcct caggacgaga cggtgagcga
     3181 gcgccggctc ggcaaacaat ttcacgtcgt cgggaagcac gtagtcgcgg ccgtcgagcg
     3241 ccgcccacgc tcgcgacaac gtgaggagcg cgagcgagcc gcgcggactg gcgccgacgg
     3301 ctacgcggcg gtcggcgcgc gtctcgttca ccagcgcgac aatgtagcgt tcgacgtcgg
     3361 gcacgacgtg gacggtctcc acgg
//
LOCUS       DBNHXB010000120        12064 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1365175, whole genome shotgun sequence.
ACCESSION   DBNHXB010000120 DBNHXB010000000
VERSION     DBNHXB010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 12064)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12064)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12064
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1365175"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="AC162G_04600"
     CDS             complement(<1..516)
                     /locus_tag="AC162G_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075127.1"
                     /translation="MTAYERDDKSIWRVLREADELFAKRGAVYDTLHRLDRRLREENI
                     PYALIGGMALISHGYERLTKDVDVLLTPEGLVAFAEKCVGRGYVPAFAGARKTFRDTT
                     NNIRIEIITAGEYPGDGRPKPVAFPDPAQASIERDGIRVIRLEELIELKLASGLSAAH
                     LLRDLADVQDLI"
     gene            complement(552..1310)
                     /locus_tag="AC162G_04605"
     CDS             complement(552..1310)
                     /locus_tag="AC162G_04605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5075128.1"
                     /translation="MKPTAQQVFGQRAAFYATSAAHKDRTVLDRVVELARPWPDALVL
                     DVATGTGHTAFALAPHVRQVIATDVTPEMLAEGGRLKDEGGMTNVQFRIADAHDLPFE
                     DETFDIVTCRRAAHHFADIAGALREMKRVLKGNGRLVIDDRSVPEDDFIDAAMNRLDW
                     LHDESHVREYRSSEWQLMLSEAGLEVEAIEPYTKHRPLSSLTAGVSPDNAAEIHRIIA
                     SLGKFQRAAMNVVEKEDEIYTDHWYVMVGVKRAT"
     gene            1500..2396
                     /locus_tag="AC162G_04610"
     CDS             1500..2396
                     /locus_tag="AC162G_04610"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR5075129.1"
                     /translation="MNEDQLLTFLTVARTRSLSRAAGELDLGQPTISDRLRALERELG
                     AALVRRQGRGVALTPEGEAFLPYAQRVLDVLKRGAESVRAAHGGSGGRASIAVTVTAG
                     AYLFAPALVAFQKEHPGVEVQVRSAHSWESPGLLLDDVVHLALTSGAPVHPQIEIVAE
                     FRGKLALVARSDHPLTRRASVAKGDLLDEQLLVSYWGPAYQRFLEDVKAGEAPRRWME
                     LSPVELVKGLLMAGVGVSIVPEVSVREEIRDQRLAMLTLADAKLPDWHIALSRRAHRA
                     ANPAADALAETLVKVLPGVTGK"
     gene            complement(2467..3501)
                     /gene="rfbB"
                     /locus_tag="AC162G_04615"
     CDS             complement(2467..3501)
                     /gene="rfbB"
                     /locus_tag="AC162G_04615"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR5075130.1"
                     /translation="MKNLLVTGGAGFIGSNYVRFVLEHHPDYRVVVYDKLTYAGNLDN
                     LIDVADRFADRYNFVHGDICDPAGVARAVREYGIDTLVNLAAETHVDRSLMAPGHFIQ
                     TDVYGTFVLLEAARSFGLERFHQVSTDETYGQVLEGSSKETDPLEPRSPYSASKAGGD
                     LMVHAYCASFGVPATITRGSNNIGPYQYPEKAVPLFITNAIDDQPLPLYGDGLQQRDY
                     QYVVDSCEGIDIVLHRGQPGEVYNLGTGVETTNIAMAKMVCDLLGKPHSLIRKVADRP
                     GHDRRYSLDVSKIGALGWESRHTFAEALEKTVKWYVENEWWWRKLKSGEYLEYYKQQY
                     GERLAKSEAL"
     gene            3954..5330
                     /gene="dnaA"
                     /locus_tag="AC162G_04620"
     CDS             3954..5330
                     /gene="dnaA"
                     /locus_tag="AC162G_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR5075131.1"
                     /translation="MKAAHVWQAALGELQLQMTKPTFETWVKPARVVAYEDGEFIIAV
                     PSAFTKDWLESKLLSSIKRALSGILGRSVDVAFIVMPEEESETPQALLYGAELTEMAG
                     ARPPNGGANGPPMMTGNLNPRYVFDTFVVGPSNRLAQAACLAVAEKPASTYNPLFLYG
                     GVGLGKTHLLHAIGHKCLAAGLQVLYVSSEQFTNELINAIRSQDTEGFRDKYRSIDVL
                     LIDDIQFIAGKEATQEEFFQTFNALHGNGKQIAMSSDRQPKSMVTLEERLRSRFEWGL
                     IADIQPPDVETRTAILRAKAETQGAAAPDEVLDFIARQVHSNIRELEGALNRVLAHVR
                     MTGGPLSVEAASVALAGLVARPPGLTVDEILLAVAHYYGVSMDDMTGRARNKESVKPR
                     QVAMYLCREEMQTTLPQIGEVLGGRDHTTVMHGVEKISREVEEDDNLRREIMAIREKL
                     YNNRNGSR"
     gene            complement(6088..6783)
                     /locus_tag="AC162G_04625"
     CDS             complement(6088..6783)
                     /locus_tag="AC162G_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR5075132.1"
                     /translation="MDTQVTWYGHAVIGIETSGKRLLFDPYLTGNPAATVKPDALNPD
                     YILISHGHGDHVGDAPDIAKRTGAMIISNDEICTWFETKHGHANVHSQHIGGGFDYDF
                     GRVKLTPALHGSALPDGSNGGNPVGFLLYIQDGKKIYNAADTGLFGDMKLIGEEGIDL
                     ALLPIGDNYTMGPDDALRAVKLIEPKIVIPIHYNTFDLIAQDARAWAERVQKAAQDRT
                     QVVILSPGESFSL"
     gene            6893..8599
                     /locus_tag="AC162G_04630"
     CDS             6893..8599
                     /locus_tag="AC162G_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR5075133.1"
                     /translation="MSAVVNDDPIQHLMQMLRGVPLFSKLRDDELRALAGITVSATFQ
                     PGQYLYRQGDDDKVMFVILSGRVALFHIDPQGVESFIEYREPGPKGWLGEAALLLGDV
                     RDVTALVVAETAVLEVEREALNRLMAGMPGLRGHLTPTPENARRVYAPHFGWQAEGEA
                     VVIFIRKHPWSLARSMLVPSALLVGAVLVASLLSAAVPALSAVFWILAILVPLPVAAF
                     VIVDWRNDFYVLTDRRLVHEEKVPIIRQRREEAPLSAVQDVQFARTSFVAALLDFGDL
                     TVETFAGSVAMKDVPQPEEVKDLIFRELERVRARTRATARKRIRDELEHRVGQKEAPT
                     VPIAVPAQEAPHRLPLWAIALGIVRYFFPPSREVVGDSVIYRKHWIVLLRSSKYPLAG
                     LLFIVAVTLIWWNRGPLIGALPDEAWIVWAVLMVVFAAWALWLFEDWRNDLYILTSSR
                     IIDIRRVPFLLQETRKEAGLDRIQTTEVVIPSPSARFLGYGDVIVRVAGVSGEFRFLN
                     MRNPSGVQTEVSKRVDQFKRRQAEEEARTRRTELSDWFATYDQIKSGYTAPASRPESG
                     ES"
     gene            8635..9201
                     /gene="def"
                     /locus_tag="AC162G_04635"
     CDS             8635..9201
                     /gene="def"
                     /locus_tag="AC162G_04635"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006556805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR5075134.1"
                     /translation="MAVREIVVVDHPVLRKKAKKVDKITPEIRRLIDDMAETMRDAPG
                     VGLAAPQVGVSQRVIVVEYAEEEEKETPDGQAPKPVKKRLYAVVNPEITRRSEEMVEG
                     VEGCLSIPGWAGSVVRHAEVEVKGLNRAGGKFKLRARGWLARIFQHEVDHLDGILFID
                     RVSGPDRIWRLPPPEAESPEEARAEHLG"
     gene            9344..10546
                     /locus_tag="AC162G_04640"
     CDS             9344..10546
                     /locus_tag="AC162G_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075135.1"
                     /translation="MTGYLTRKGMRFVAIVVAGVVAALLLWLATNVFNVPLPGVDTSP
                     LDVQLFVAPQAFDWVSEAAERFNAEGRRLNHRPIVIYVVERDGLSLHAQVSSASLNPA
                     PTAWIAEGAFTLDLVNLAARQSGISNAFTAERSVAQSVMMWGGFADRIAALDSNFGGL
                     TWKAIHEAAIARSGWSSLGGQPEWGFFKLVLADPSKSSEALAALLAAAAEYHGKTSLT
                     AADVNDARFQQWAQDLVDAVPNFANLGSEPGKALAVRGASAGDAGQLLEAEWLSAAEG
                     MGNWQTPAFRYAPTAVIFDFPFGVWSGPGGDPSASLRAGAAEDSSRRDAARLFRDVLL
                     QDAQQRRAEAFGLRPASGNVANAEGSLFARWASLGFESGRPPTASAQASADAVLAALR
                     WAERAAGR"
     gene            10668..>12064
                     /locus_tag="AC162G_04645"
     CDS             10668..>12064
                     /locus_tag="AC162G_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5075136.1"
                     /translation="MRYMLVSRLLVAILTLAIFAAACDGGGLVEGTPVVDRPANAIEV
                     SIVYAPEFAPFMPAVINDFNRAFAEGRHPVTGQPMGPGERPIYVTGKDGSSGTVMQGI
                     VNAVIAPGNVNVERPTLWSPSVSHWLALANYNAGRELFDLTDSPPTALAPVVMAIWES
                     RLNAIQAKNPGEAIGWEHLLEVLNDPNGWASYGIPGRSTVYYGHTDPYISSTALSTLI
                     AEFYASARYNSNNPNLRRLTLDVINDPRVQEGVRRIEQLIKHYSARTTEFKEYIAQGP
                     EYLDFVALEENDLIYINQGKTQYQPPERLVALYPKEGTFWHEHPFAIPNADWVTPEQR
                     AAAKVFTEYVLSKPVQEIILANGFRPASPDVPAGYPIVAELGVDPAQPATVLDVPDPA
                     VVAAVQSSWQFVKKQADVLLLFDVSGSMSGDKIDQARQAALAFLDKLPAQNRVGLMTF
                     SHIIDQRVPLDSFER"
BASE COUNT         2340 a         3644 c         3695 g         2385 t
ORIGIN      
        1 gatcaagtcc tgcacgtcgg ccaggtcgcg cagcagatgc gccgccgaca gtccagacgc
       61 cagcttcaac tcgatcaact cttccaatcg gatgacgcga atcccgtcgc gctcgatcga
      121 cgcttgcgcc ggatcgggaa aagcgaccgg tttgggcctg ccatcacccg gatactcgcc
      181 cgccgtaata atctcgattc gaatgttgtt cgtggtatcg cggaatgtct ttcgcgcgcc
      241 tgcgaaggca gggacgtagc cgcggccaac gcacttctcg gcgaaggcca ccagcccttc
      301 cggagtcaag agaacgtcaa catccttcgt gaggcgttca tagccatgcg aaatcagagc
      361 catgccgcca atcaatgcat acgggatgtt ttcttcgcga agtcgccgat cgagtcgatg
      421 cagcgtgtca tagacagcgc ctcgctttgc gaaaagctcg tcggcttccc gcagcacccg
      481 ccagatcgat ttgtcgtctc tctcgtaggc agtcatatcc aaaagtatac catcagccca
      541 cgcatcatgc atcacgtagc acgtttcaca cccaccatca cgtaccaatg atccgtgtat
      601 atctcgtctt ccttttctac gacattcatc gccgcgcgct ggaacttgcc gagcgaagcg
      661 atgatccggt gaatctcagc ggcgttgtcc ggcgagacgc cggccgtcag ggacgataat
      721 ggccgatgct ttgtgtacgg ctcgatcgct tcgacctcaa ggccagcttc ggacaacatg
      781 agctgccatt cgctcgacct gtattctcgc acatgcgact cgtcgtggag ccaatcgaga
      841 cgattcatgg ccgcgtcgat gaagtcgtct tccggcacgc tgcgatcgtc aataaccaat
      901 ctcccattac ctttcagtac gcgcttcatt tcacggagcg ccccggcgat gtccgcaaag
      961 tgatgcgccg cgcggcggca ggtcacgatg tcgaatgtct catcttcgaa aggcaaatcg
     1021 tgcgcgtccg cgattcggaa ttggacattg gtcattccgc cttcatcctt caaccttccg
     1081 ccttccgcaa gcatctccgg cgtgacgtcg gtcgcgatca cctgccgcac gtgcggcgcg
     1141 agcgcaaacg ccgtgtgacc ggtccccgtc gccacgtcga ggacgagtgc atcgggccac
     1201 ggtcgcgcca attcgacgac gcgatctaga acggttctgt ccttatgagc cgcgctggtc
     1261 gcataaaagg cggcgcgttg gccgaagact tgttgagcgg tgggtttcat atttacctcg
     1321 cgacaacgcg atacgtgttg cgtgttgcgt cacaagcgcc gttcagctac attctccagt
     1381 ataatcagta ccgtatccga tacaagcacc tgtcagcgat agcacctatc ggaaaacccg
     1441 ataaccttca cgcatcacgc attacgtctt acgtattacg catcacgtat cacttctgaa
     1501 tgaacgaaga ccaactcctc accttcctca ccgtcgcccg cacgcgcagc ctcagccgcg
     1561 ccgccggcga gcttgacctg ggccagccga cgatcagcga tcgtctgcgg gcattggaac
     1621 gcgagcttgg agcagcgcta gtgaggcggc agggccgcgg cgtcgcgctc acccccgaag
     1681 gagaggcgtt cctgccctac gcgcagcgcg tgctggacgt gctgaagcga ggagcagaat
     1741 cggtccgggc cgcgcacggc ggctcgggcg gccgtgccag catcgccgtg accgtcactg
     1801 ccggcgcgta tctcttcgcg cctgcgctcg tcgcgttcca gaaagagcat cccggcgtcg
     1861 aagttcaggt acgctcggct cactcgtggg aatcgcccgg cctgctgctc gacgacgtgg
     1921 tgcatctcgc gctcacgtcg ggcgcgccgg tgcatccgca gatcgaaatt gtcgccgaat
     1981 ttcgaggaaa gttggcgctc gtcgcacgga gcgaccaccc gctgacgcgg cgcgcgagtg
     2041 tcgccaaagg cgatttgctc gacgagcagt tactcgtctc gtactgggga ccggcttacc
     2101 agaggttcct cgaagacgtg aaggccggtg aagcgccgcg ccgctggatg gagcttagcc
     2161 cggtcgagct ggtcaaagga ttgctgatgg cgggggtggg ggtgagtatt gtgccggagg
     2221 tctcggtgag ggaagagatt agagatcaga gattggcgat gctcaccctc gccgacgcga
     2281 aactgcccga ctggcacatc gcgctgtcgc ggcgagcgca tcgagcggcg aacccggcgg
     2341 cggatgcgct ggcggagacg ttggtgaagg tgttgccagg tgtaacggga aaatgagatg
     2401 caaaaagccc tacgcaggag ttgcctccac acagggcctg tcacgcatca cgcttcacgc
     2461 gtcacgctac agcgcctcgc tcttcgccaa ccgctctccg tactgctgct tataatactc
     2521 cagatactcg ccgctcttca gcttgcgcca ccaccactcg ttctcgacgt accacttgac
     2581 cgttttctcc agcgcttcgg cgaacgtgtg ccggctctcc cagcccagcg cgccgatctt
     2641 gctcacgtcg agcgagtagc gccggtcgtg gccgggccgg tcagcgacct tgcgaatgag
     2701 cgagtgcggc ttgcccagca gatcgcacac catcttcgcc atggcgatat tcgttgtctc
     2761 gacgccggtg cccaggttgt acacttcacc cggctggccc ctgtgcagca cgatgtcgat
     2821 accctcgcaa gagtcgacca cgtactggta atcgcgctgc tgcaggccgt cgccgtagag
     2881 cggcaagggt tgatcgtcga tggcgttcgt gataaaaagc ggcacggcct tctcgggata
     2941 ctgatagggg ccgatattgt tgctgccacg cgtgatcgtc gccggcacgc cgaacgaagc
     3001 gcagtaagcg tgcaccatca ggtcgccgcc agccttgctg gccgagtacg ggctgcgcgg
     3061 ctcgagcggg tcggtttctt tcgaggagcc ttccaagacc tgcccatacg tttcgtcggt
     3121 gctcacctgg tggaaacgct ccagcccgaa gctcctcgcg gcttcgagca gcacgaaggt
     3181 gccgtacacg tcggtttgaa tgaaatgccc cggcgccatc agcgagcggt cgacgtgtgt
     3241 ctcggcggcg aggttgacaa gggtgtcgat gccgtattct cgaacggctc tcgccacgcc
     3301 ggccgggtcg cagatgtcgc cgtgcacgaa gttataacga tctgcaaacc gatcggccac
     3361 gtcgatgaga ttgtcgagat tccccgcgta cgtcagctta tcgtacacga cgactcgata
     3421 atcggggtga tgctcgagca cgaagcgcac ataattggag ccgatgaatc ccgctccgcc
     3481 ggtgacgagc aggttcttca tcgcgctctc gtcctcgaat ttgactctgg tccgtctttc
     3541 atttagggtc tttcctcaaa cgatgtggat ttccatgtgg acaagtggcg cgatggcgtg
     3601 gataagtgtg cctggttgtg gataattcta ccatgcgcgg ggaaagccgt gaagatcgac
     3661 cgccggggac tctcgatccg catcgaagct taaaaaatcg tgagttttaa ggttgggctt
     3721 cgatcaacct attgccaaac accatctctt gctgtagaat gtgcccgcac gttcggactc
     3781 aggcaggaga ttcaacacac acaaccagcc tattcggcca gagcctttga ctggcggcgg
     3841 cacaactgcc gccaattttt ttccctcaac cggaagtcga ttacgctgtt tgaagagagc
     3901 cttgtcgcat gtctacccac atctctttct caccccgcac ggagtctgtc gaaatgaagg
     3961 ccgctcatgt ctggcaagcc gcactcggcg agctgcagtt gcagatgacg aagccgacat
     4021 ttgagacatg ggtcaagccg gctcgagtcg tcgcttacga ggatggcgag ttcatcattg
     4081 ccgtccccag cgcgttcacc aaagactggc tggaaagcaa gctgctttcg tcgatcaagc
     4141 gagcgctgtc cggaattctc gggcgttcgg tggacgtcgc cttcatcgtg atgcccgaag
     4201 aagagagcga gacgccccag gccctgctct acggcgccga gctcaccgag atggccggag
     4261 cgcgcccgcc gaacggcggc gcgaacggcc cgccgatgat gaccgggaac ctgaacccgc
     4321 gctacgtgtt cgacacgttc gtcgtcggcc ccagcaaccg gctggcgcag gcggcctgcc
     4381 tggccgtcgc cgagaagccg gcaagcacgt acaacccctt gttcctatac ggcggcgtcg
     4441 gcctgggtaa gacacacttg ctgcatgcca tcgggcacaa gtgtctggcg gctggtctgc
     4501 aagttctgta cgtctcgtcc gagcagttca cgaacgagct gatcaacgcc atccgctccc
     4561 aggacacgga gggctttcgc gacaaatatc gctccatcga tgtgctgctg atcgacgaca
     4621 tccagttcat cgccggcaag gaggcgacgc aggaagagtt cttccagacc ttcaacgcgc
     4681 tgcacggcaa cggcaagcag atcgccatgt cgagcgaccg gcagccgaag tcgatggtca
     4741 cgctcgaaga acggctgcgc agccgcttcg agtggggctt gatcgccgac attcagccgc
     4801 ccgacgtcga aacccgcacc gccatcttgc gcgccaaggc cgaaacgcaa ggcgctgccg
     4861 cgcccgacga ggtcctcgac ttcatcgcgc ggcaggtgca cagcaacatc cgcgagctgg
     4921 aaggcgcgct gaaccgcgtg ctcgcgcacg tgcggatgac gggcggcccg ctcagcgtcg
     4981 aggcggccag cgtcgcgctg gccggcctgg tcgcccgccc gccgggcctc accgtcgacg
     5041 agatactcct ggccgtggcg cactactacg gcgtttcgat ggacgatatg accgggcgcg
     5101 ctcgcaacaa ggaatcggtc aagcctcgcc aggtcgccat gtacctgtgc cgcgaagaga
     5161 tgcagacgac cctgcctcag atcggggaag tgctcggcgg ccgcgatcac acgacggtca
     5221 tgcacggcgt cgagaaaatt tcgcgggaag tcgaagaaga cgacaatcta cgacgcgaga
     5281 tcatggcgat tcgcgagaag ctctataaca acaggaatgg cagccgatag cctgccctga
     5341 acataacata actgatcaat cgatagcatc caagcacggg tggttggctc aaaacgggga
     5401 aacgagggta tttattgccc tcgttttctc ttggcccagt acccagaagc gccaaaggtt
     5461 ggcaaataat ttgtggataa gtggctaata actgtggata aacgagttgc tttggggata
     5521 actcccgcgc ccgaaaactt tatgtcatgt cgactatcca tatatggggg tatgacagcg
     5581 acagaagtcc aaaatattga cagggagtcg aaacgacata agtgacagtt cgtgtatcca
     5641 catcatactc accacttgag atggtttaag ttggaaggaa gtcgctagat acggtggtgg
     5701 cagcactcgg gataccctta tctagcgcac aatgaagcgc catccacaaa tccacgtccc
     5761 ctactactat gactatattc aatacctttt atctacaatg tcatacagtt tcttcagata
     5821 cttggacgtg aagctgctcg aaacgtgagg gtcacctgtc gtagatagcc atcctggtct
     5881 tggcctcgga tgaatatttc ttcgacgtga cctctggagc cgctgttcag gcatagctcg
     5941 aacagctcgt ccaaagaggt gaagggagca ctggtctgaa tcagtccccc ttccggagtc
     6001 cattgcagat aaacgacggt cccgccttcg gaatgtgcgg ccatacgtcg ctccgaggtg
     6061 taatgagtcg gcatggcatg caccggccta aagagaaaac gactccccag gtgaaaggat
     6121 gacgacctgg gtcctatctt gcgctgcttt ttggacgcgc tcagcccatg cgcgagcatc
     6181 ctgcgcaatc aagtcgaacg tattgtagtg gataggaatg acgatcttag gctcgatgag
     6241 cttcacggcg cgcaaggcat cgtccgggcc catcgtgtaa ttatctccga tcggaagcag
     6301 ggccagatcg atgccttctt caccgatgag cttcatgtca ccgaacagcc ccgtatcggc
     6361 ggcgttgtag atctttttcc cgtcctggat atacagcagg aatccgaccg gattgcctcc
     6421 gttcgaccca tccggtaacg ccgagccgtg cagggccggc gtgagtttca ctctcccgaa
     6481 atcataatcg aacccgccgc cgatgtgctg ggagtgaaca ttcgcgtgtc cgtgtttcgt
     6541 ctcgaaccat gtgcagatct catcattgct gatgatcatc gcgccggtgc gtttggcgat
     6601 gtcgggcgcg tcgccgacgt gatcgccgtg gccatgcgaa atcaagatgt agtcggggtt
     6661 cagcgcatca ggcttcaccg tcgcggccgg gttgcccgtc agatagggat cgaacaagag
     6721 gcgcttgccg ctggtctcga tgccgatcac ggcgtgcccg taccaagtga cttgtgtatc
     6781 catgttcacc ttccaggaag acagaatcgg attgctatga gtataagcgt gcaggcatcg
     6841 tctgtcaatg cgactttggg cgagacgaaa cctgtgctac aattttgcac gtatgtctgc
     6901 agtcgtgaat gacgacccaa tccagcatct aatgcagatg ctacgaggag tgccgctgtt
     6961 ctctaaactg cgcgatgatg agctgcgtgc gctggcgggt atcactgtgt cggcgacatt
     7021 tcaacctggc cagtatctgt atcgccaggg cgacgacgac aaggtgatgt ttgtgatcct
     7081 atccggccgg gtggcgctgt tccacatcga tccgcagggc gtggagtcct ttatagagta
     7141 tcgcgagcca ggtccaaagg ggtggctggg cgaagcggcg ctgctgctgg gtgatgtgcg
     7201 cgatgtgaca gccctggtcg tcgctgaaac ggccgtattg gaggtcgagc gcgaggccct
     7261 caatcgactg atggccggca tgcctggcct gcgcggtcat ctcacgccta cgccggaaaa
     7321 tgcgcgcagg gtgtatgcgc cgcattttgg ttggcaggct gaaggcgaag cggtggtgat
     7381 ctttatccgc aagcatccct ggtcgctcgc tcggtcaatg ctggtgccat ccgcgctgtt
     7441 agtcggggcg gtgctggtgg cttctttact cagtgcggcg gtgcctgctt tgtcggccgt
     7501 tttctggata ttggcgatat tggtgccact cccggtcgcc gctttcgtca tcgtcgactg
     7561 gcgcaacgat ttctacgtgc tgaccgacag acggctcgtg catgaggaga aggtacccat
     7621 cattcgccaa aggcgtgagg aagcaccact ctcggccgtg caggacgtgc aattcgcccg
     7681 tacctccttc gtagctgccc tgctcgactt tggcgacctg acggtagaaa cattcgccgg
     7741 cagcgtggct atgaaagacg tgccgcagcc ggaagaggtg aaggacttga ttttccgcga
     7801 gctcgagcgc gtgcgtgcgc gcacacgagc caccgcccgc aaaaggatcc gcgacgagct
     7861 tgagcatcgt gtcgggcaaa aggaagcccc gacggtgccc atcgcagttc cagcgcagga
     7921 agcgcctcac agactgccct tatgggctat cgcgctgggg atcgttcgct actttttccc
     7981 cccgtcacgc gaggttgtcg gcgactcggt gatctaccgt aagcactgga tcgtgctgct
     8041 ccgtagcagt aagtacccgc tcgccggcct ccttttcata gttgccgtca ccctcatctg
     8101 gtggaatcgc ggacctctta tcggcgctct acccgatgag gcttggatcg tatgggcggt
     8161 gttgatggtg gtgttcgctg cgtgggcgct atggttgttt gaggactggc gaaatgatct
     8221 ctatatcttg accagcagcc ggatcatcga cattcggcgc gtaccatttc tgctgcaaga
     8281 aacgcgcaag gaagcgggac tggatcggat acagacgacc gaagttgtca tcccatcgcc
     8341 gagcgctcgg tttctaggct acggtgacgt aatcgttcgc gtggcgggcg tgagtggcga
     8401 gtttcgattt ttgaatatgc ggaatcccag cggtgtgcag actgaagtga gcaagcgtgt
     8461 cgatcagttc aagcgccgcc aggcggagga agaggcgcgc actcgtcgca ccgagctgag
     8521 cgactggttt gccacgtacg atcagatcaa atccgggtac acggcgccgg cgagccgtcc
     8581 cgagtccggc gagtcgtagg ggccgggctt gcccctgccc ctatgagagg aaccatggcc
     8641 gttcgtgaaa tcgtcgtcgt cgatcacccc gtcctgcgca agaaggccaa aaaggtcgac
     8701 aagatcacgc ccgagattcg acgcctgatc gacgacatgg ccgagaccat gcgcgacgcg
     8761 ccgggcgttg ggctggccgc gccgcaggtc ggcgtgagcc agcgcgtcat cgtcgtcgag
     8821 tatgcggaag aagaagaaaa ggagacgccg gacgggcagg cgcccaaacc ggttaagaag
     8881 aggctgtatg ccgtcgtcaa cccggagatt acgcgccgct cggaggaaat ggtcgaaggc
     8941 gtggagggct gtttgagcat ccccggttgg gcgggcagcg tcgtgcggca cgcagaagta
     9001 gaggtcaaag ggctgaaccg cgccggcggc aaattcaagc tccgtgcgcg cggttggctc
     9061 gcgcgcatct tccagcacga ggtcgatcac ctcgacggca tcctctttat cgaccgtgtc
     9121 agcggccccg acagaatctg gcggcttccg ccgcctgagg cggaatcacc cgaggaagcg
     9181 cgtgctgaac acttgggata agagccgcct cacgcggtgt ttttgcgttc ccccgcgcct
     9241 tgctgtcagg cgcttgtcgg cccaatgtga ttgactgcgc ccggccggta tgctattctg
     9301 atgcgtgact tgtgagtggc ccaccggtaa acaggatgcg caaatgacag gatatttgac
     9361 ccgtaaaggg atgcgcttcg tcgccatcgt cgtcgccggc gtcgtcgcgg cgcttttgct
     9421 gtggctggca accaacgtat tcaacgtccc gcttcccggc gtcgatacct ccccgctcga
     9481 cgttcaactg ttcgtcgccc cgcaggcgtt tgactgggtt tcggaagcgg cggagcgatt
     9541 caatgccgag ggccgccgcc tcaatcaccg cccgatcgtg atatacgtcg tcgagcgaga
     9601 tgggctgagc ctccacgcac aggtcagctc ggcgagtttg aatcctgcgc cgacagcctg
     9661 gatcgcagag ggagctttca cgctcgacct ggtcaacctg gcggccaggc agtcgggcat
     9721 cagcaacgca ttcaccgccg agagatcggt cgcgcagtcg gtgatgatgt gggggggatt
     9781 cgccgatcgg atcgctgcgc tggatagcaa cttcggcgga ttgacgtgga aggcaataca
     9841 cgaggcggca atcgcgcgga gtggctggtc gagcctgggt gggcagccgg agtggggttt
     9901 cttcaagctg gtcttagctg acccgagcaa gagtagtgag gcgctggcgg cactcctggc
     9961 tgcagcggcg gaatatcacg gtaagacgag cctcacggca gccgatgtga acgacgcgcg
    10021 ttttcagcag tgggcgcagg acctggtcga tgccgttccg aactttgcca atctcgggtc
    10081 cgagccgggc aaggcgctcg ccgtgcgcgg cgcatcggcc ggcgacgccg gacagcttct
    10141 cgaagccgag tggctgagcg cggcggaggg aatgggcaac tggcagacgc ccgcctttcg
    10201 gtatgcgccc acggcggtga tattcgactt cccgttcggc gtgtggagcg gcccgggtgg
    10261 cgacccttcg gcttcgctca gggcaggcgc tgcggaagat tcctcgcgcc gggacgccgc
    10321 ccgtctgttc cgagatgtcc ttctgcaaga tgcacagcag cgccgcgccg aggcattcgg
    10381 acttcgaccc gcgtcgggga atgttgccaa cgccgaaggc agcctcttcg cccggtgggc
    10441 ttctctcgga ttcgaatcgg gccggccgcc gacagcgagc gcccaggcca gcgccgacgc
    10501 cgtgctggcc gcattgcgct gggctgagcg cgccgccggc cgctaagagc ctatccgaag
    10561 accgtaaggc ggcatcccca tgccgcttgc tggcgcatag ggatgcacca gctacgggtt
    10621 tccgaaaacc gtagggcggc atccccatgc cgttaaggag gtgtacgatg agatatatgc
    10681 tcgtgagtcg gctcctggta gcgattctga cgttggcgat attcgcggcc gcctgcgatg
    10741 gcggtggtct ggtcgaaggc acgccggtcg tcgatcgacc ggccaatgca atcgaagttt
    10801 ctattgtcta cgcgcctgag ttcgcgccgt tcatgccggc ggtgatcaac gatttcaacc
    10861 gcgccttcgc cgagggtcgc catccggtga ccggccagcc gatggggcca ggcgagcgcc
    10921 cgatctacgt caccgggaag gacggctcga gcggcacggt gatgcaaggc atcgtcaacg
    10981 ccgtcatcgc gccaggcaat gtcaacgtcg aacggccgac gctgtggtcg ccgtcggtca
    11041 gtcactggct ggcgttggcg aactacaacg ctgggcgcga gctcttcgac ctgaccgaca
    11101 gcccgcccac cgcgctcgcg ccggtggtga tggccatctg ggagtcgcgc ctgaacgcca
    11161 tccaggcgaa gaatcccggc gaagccatcg gctgggagca tctgctggag gtgctcaacg
    11221 atccgaatgg ctgggcctcg tacggtattc ccggccggtc gacggtgtat tacggtcaca
    11281 ccgatccgta catcagcagc accgcgctct cgacgctgat cgccgagttc tacgccagcg
    11341 cgcgctataa ctcgaacaac ccgaacctgc gccggctgac gctggatgtc atcaacgatc
    11401 cgcgcgtaca ggagggcgtg cgccgcatcg agcagttgat caagcactac tcggcgcgca
    11461 ccacggagtt caaagagtac atcgcgcaag ggccggaata tctcgacttc gtcgcgctcg
    11521 aagagaacga cttgatctac atcaaccagg gcaagacgca gtaccagcca ccggagcgcc
    11581 tggtggcgct gtacccgaag gaaggcacct tctggcacga gcatcccttt gccatcccga
    11641 acgccgattg ggtgacgccc gagcagcgcg ccgccgccaa agtgttcacc gagtacgtgc
    11701 tgtcgaagcc ggtgcaggaa atcattctgg ccaatgggtt ccgcccggca agccctgacg
    11761 tcccggccgg ttatcccatt gtcgccgagc tgggcgtcga tccggcgcag ccggcgacgg
    11821 ttctcgacgt gcccgatccg gcggtcgtcg cggccgtgca gtcctcgtgg cagtttgtga
    11881 agaaacaggc cgacgtcttg ctcctgttcg acgtctccgg ctcgatgtcg ggcgacaaaa
    11941 tcgatcaggc gcggcaggcg gcgctggcct tcctggacaa gctgccggcg cagaaccgcg
    12001 tcgggctgat gacgttcagc cacatcatcg accaacgcgt gccgctcgac tccttcgagc
    12061 gtaa
//
LOCUS       DBNHXB010000121        11310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_862011, whole genome shotgun sequence.
ACCESSION   DBNHXB010000121 DBNHXB010000000
VERSION     DBNHXB010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11310
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_862011"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="AC162G_04650"
     CDS             complement(<1..196)
                     /locus_tag="AC162G_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075137.1"
                     /translation="MSHRLRITLALAVFLVLSSATPALAGCCAIVTLDALPAQIVAGQ
                     PVRISFIYRVNKFPRQAGKPE"
     gene            complement(236..1039)
                     /locus_tag="AC162G_04655"
     CDS             complement(236..1039)
                     /locus_tag="AC162G_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5075138.1"
                     /translation="MLRLSILKLLLVIIVVLAIAAPARAGGWAVVTLDQLPAQVVAGE
                     PLTIGFMVRQHGRTPVDGLTPRVTATRAGINESFTVTAKPQGDIGHYAAALAFPGTGT
                     WNWTIDAFGFAQPMPPLTVVAAASSSSAGAGASSPLVSMRTRSRWALALVAVAALVSV
                     TGFVPAAGEGARVAPLVGQPISSEFGQALFIAKGCVVCHQHEAVREARKEFAGFSVGP
                     NLTQRELGPEYLRHWLKDPSAIKPRTEMPTLDLSDDEIEALVAFLTTSR"
     gene            complement(1057..1638)
                     /locus_tag="AC162G_04660"
     CDS             complement(1057..1638)
                     /locus_tag="AC162G_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5075139.1"
                     /translation="MDERQLIERARRGDVGAYDELVRAHQEAAFRAAYMITRDVADAQ
                     DAAQEAFIRAYDALGRFDARRPFRPWILRIATNAALNRVKASMRRQAMAERFGAGTVE
                     REANPTIEARVLRRERGRRVQDAIRHLKPDDQSLLILRFFFDLSEAELAQTFDVAAGT
                     IKSRLHRALAKLRDVIRKHDPDLLESDMQTAGE"
     gene            complement(1796..5851)
                     /locus_tag="AC162G_04665"
     CDS             complement(1796..5851)
                     /locus_tag="AC162G_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5075140.1"
                     /translation="MADVPAALTASSERPAARYSRRFVLLSLIAAAAVYVAAPLLAYR
                     WAGQPVFLGVMLEQTFNVNDSRGTGWGPSPDGLNVQDHVESINGRTIDSQAALDRALA
                     EVAADPGRRAQIAYLRRTPDGTLQPGSITASLGPFPQGDLLRLFWLPYLIGLVYLGIG
                     LWVFRLRGHQRAGLTFTFFSACVAVTLAVFFDMNTTHVLLVLWALGLALSGGAMIHLA
                     LVFPQEPRFVLRRPVLRLLPYVPVAALVAYTLTVQPDLSQPWDYITAWRLLYLFIALG
                     LVVFYGMLVYRRLGSTSPVVRQQSRVILLGSVVAFAPFALWIIAGIVGVTAPFDPLIY
                     FPPLVLFPLSIAYALVRYHLLDVDLILNRALVYAIVVALVVGAYFLTIAVIGALAQNT
                     QALMSNPSLLAVFVLIVAVLLDPLRKRVQLAVDRMFFPVRIDVHGLLQTYSHDLTGAA
                     DLASIVSLLAAAIGETLKPDPLRVYLGDPRVRSYLLHASQGETGKPPPLLARCPWDSP
                     LARWLSDRHEPWYIQPDRPLPEPIAGESARLEAMGASLVVPLHGRDRLNGWVALGAKA
                     SGQPYTTDDLSFLEALADQTTLALERAMTHADLQRRVTELNALSQVSQGVNFKVDPDA
                     TLELIYAQASKVLDTSNFYIALADLSRNTMRFAFYVENGERLKPDDEWPLDVGLTGAI
                     VRTGQPIVATDYVQECLRRGITPGGKPGRAWMGVPLNAGDQVLGVMNVSSFDPDVTYG
                     AEQLQIFSAIADQAASVLEKIRLFRITEDRAQQLAVLNEVSASITSSLDLRTVLNRIM
                     EKAVEILGAEAGSLLLVDETTQELVFEVILGSSKDQLAGTRLPPGAGIVGSVAQSGRP
                     LIVNEAQRDERWLRDVGQTDQFMTRALLTVPMKSRGKVMGVIQVLNKRDGTPFSEDDQ
                     TLLESFAGNAAVAVENAKLFNRTDLALARRVEELSTLQEIDRELNTSLDFKRVMRLTL
                     DWGLRVSGAEAGSIGMIDREQNALLILASRDYSGADASDDGKALPLDRGLAGKSIRTG
                     LPLLLDDTTPDPEGAPASPETRSQISVPIRRGSEVVGVLNLESFQPRAFSTVEFESAI
                     RLADHAATAIANARLFEEVKRANDDKSEFVSTVSHELKTPMTSIKGYTDLMIKGAAGS
                     LTDVQQQFLNTVRSNVDRMSDLVNKLLDLSRIETGRLKLDIKPVSMEDVIEETLRTTR
                     GQIEARQQKLDIAVPRDLPMVMGDRASLIQIMTNLISNAHKYTPAGGHIVVSAQPRSG
                     GSPGFVLCAVRDTGVGISEEDQAKLFTKFFRAGDPAVREMPGTGLGLAITKSLIEMHG
                     GEIWVESRVGKGSTFAFTVPVAKPSGRPVVA"
     gene            6159..6557
                     /locus_tag="AC162G_04670"
     CDS             6159..6557
                     /locus_tag="AC162G_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR5075141.1"
                     /translation="MDRKIRVLIAKPGLDGHDRGAKVIARALRDAGMEVIYTGLRQTP
                     EMVAEAALQEDVDVVGLSILSGAHMTLVPRVVDLLKQKGLDDVLVFVGGIIPDEDVAP
                     LKALGVRGIFGPGANTTEVARFITEQVGSA"
     gene            6559..7575
                     /gene="meaB"
                     /locus_tag="AC162G_04675"
     CDS             6559..7575
                     /gene="meaB"
                     /locus_tag="AC162G_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR5075142.1"
                     /translation="MRQARRSPRELVDGALTGDRRALARLISLLEGDSQAAAECMVHL
                     HPRAGRAAIVGITGAPGTGKSTLVNELAKAFRRRGRTVGVIAVDPTSPFSGGAILGDR
                     IRMRDLSGDDGVFIRSMATRGSLGGLARATADVACTLDAAGFDVILIETVGAGQSEVD
                     IARTADTVLVVEAPGLGDEVQAIKAGILEIADVLVVNKADREGAENTFRTLQAMLDLG
                     AAPRAVRHHGRVMEVPDSSPADDQPSGWRPPIVKTVAIEGKGTDELIAAIDGHQAHLE
                     ASGEGAARRRQRAETELETILRETLLQRLRKQVGRVGLDGVVDRVASRELDPYTAART
                     LIDG"
     gene            7671..8333
                     /locus_tag="AC162G_04680"
     CDS             7671..8333
                     /locus_tag="AC162G_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR5075143.1"
                     /translation="MSVRLYLVRHAESTWNAEGRVQGQADPPLSERGLAQAERLAERF
                     RGEPIVALYSSPLERARQTAERIAEATALATQIDNRLKEHDMGLFTGLVWREIVQQFP
                     EFARAWMERAMDMPGGERQAAFHMRATGVMQDIAARHSDGKIVVVSHGGILGEYLAHL
                     LGLDPERRPPFRFDNASVSVVEVGGPLPALRVHRLNDVSHLIAPSGDGRRIMGDADEV
                     DG"
     gene            8330..9292
                     /locus_tag="AC162G_04685"
     CDS             8330..9292
                     /locus_tag="AC162G_04685"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR5075144.1"
                     /translation="MRSVTRAYSDIRIFAGSGSRHMAEEVCRYLNVPLSPCEVIRFPN
                     ENIFVRLQASVRGQDVFVIQGTSSPVSDNIMELLILLDTVKRANAGRITAVIPYFAYG
                     RSDKKDQPRVPITARLLADMITAAGADRFLVLDLHAGQIQGFFSIPGDELTTFHLLSD
                     YMLTKDLSNAVVVTADLGFAKKGRNFAHKLNLPMAFVEKRRTGNDARAQALSVIGDVR
                     GKDVIIVDDEVDTAGSVSDAVRIVKENGAADVYLSFVHAVLSGPAVDRLRALPIKEII
                     TTNTVPIPESKQLPNMKILSVAPLLGEVMRRIHEAVSVGQMFNE"
     gene            9331..9618
                     /gene="gatC"
                     /locus_tag="AC162G_04690"
     CDS             9331..9618
                     /gene="gatC"
                     /locus_tag="AC162G_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR5075145.1"
                     /translation="MALTREQVDHIAELAKLALTDAEKARFQEQLSDILDYAQRLQAL
                     NTDAIPPTANVLPLRSVMRDDVAAPSTPTPVLLANAPKASGDAFEVQAVLD"
     gene            9706..11175
                     /gene="gatA"
                     /locus_tag="AC162G_04695"
     CDS             9706..11175
                     /gene="gatA"
                     /locus_tag="AC162G_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR5075146.1"
                     /translation="MSLYALTIHAARERLARKEISSVELTQAMLDRIAAVDGRVHAFL
                     QVSDHMALAQAHEADARRTRGEDTPLLGIPLAIKDVICVEGMRCTCGSRILESFVPFY
                     NATVVERLRAQGAVILGKTNTDEFAMGSSTENSAFGPTRNPWNLERVPGGSSGGSAAA
                     VASEMALGALGTDTGGSVRQPAALCGVVGVKPSYGRVSRYGLVAFASSLDQIGALGKD
                     VRDAADLLGAIAGHDPRDSTSMPVGVSDYSAALKLDLKGVKVGVPKEYFIEGMQPDTE
                     QAVRAAIDVMRSLGAEIREVSLPHTDYGLPVYYLVAPAEASANLARYDGVRYGLRLSP
                     AGGPDDGLWATYRATRGAGFGPEVKRRIMLGTYVLSAGYYDAYYLKAQKVRTLIKADF
                     DAAFKEVDVIACPVAPTTAFKIGEKVDDPLQMYLSDVFTLNANLAGICGISLPCGFDA
                     QGLPIGLQINGPAFGEEKMLRVAYAYEQATEWHRQRPAL"
     gene            11172..>11310
                     /locus_tag="AC162G_04700"
     CDS             11172..>11310
                     /locus_tag="AC162G_04700"
                     /inference="COORDINATES: protein motif:HMM:NF017020.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR5075147.1"
                     /translation="MNDEQDVGGWIQYAQADRHAAQSLFASGDYSVCAQMCQQMLEKI
                     IK"
BASE COUNT         2046 a         3582 c         3741 g         1941 t
ORIGIN      
        1 tttcgggctt gccggcttgc cgcgggaact tgttaacgcg gtatatgaaa ctgatgcgaa
       61 ccggctgccc ggcgacgatc tgagccggca gcgcatcgag cgtgacgatg gcgcagcagc
      121 cggccagtgc cggtgttgcc gatgacagga cgaggaagac ggccagcgca agtgtgatcc
      181 tcagtcggtg tgacatcttc gcttcccctt tccaaagcac cttgcccatg gtccatcagc
      241 gacttgtagt cagaaacgcg acgagcgcct cgatctcgtc gtcactcaga tcgagcgtcg
      301 gcatttcggt gcggggcttg atggccgagg gatcctttag ccagtggcgc aggtactccg
      361 ggccaagctc tcgctgtgtc aggttcggcc ccacgctgaa gccggcgaat tcctttcgcg
      421 cctcgcggac ggcctcgtgc tgatggcata ccacgcagcc cttggcgatg aacagcgcct
      481 ggccgaattc cgatgaaatc ggttgcccga cgagcggagc gacacgcgcc ccctcgcctg
      541 cagcggggac aaatccggtc acgctgacca atgcggcaac cgcgaccagc gccagtgccc
      601 agcggcttcg cgtgcgcatc gataccagcg gcgaggatgc gcctgcgccc gcgctgctcg
      661 acgaggcagc ggcgacgacc gtaagcggcg gcatcggctg cgcgaaacca aacgcgtcga
      721 tggtccagtt ccacgtccct gtgcccggaa acgccagtgc cgccgcgtag tggccgatat
      781 cgccttgcgg cttggccgtg acggtgaagg attcgtttat gccggcgcga gtcgccgtaa
      841 cccgtggggt caacccatcc acgggcgtcc gcccgtgctg cctcaccatg aagccgatgg
      901 tcagcggctc tccggcgacg acctgcgccg gaagttgatc gagcgtgacg acggcccaac
      961 cgccagcgcg ggccggcgcg gcgatcgcca gtacgacaat aatcaccaac aggagtttca
     1021 aaatcgataa acgtaacatg acactctcct ccccggttac tcgccggcag tctgcatgtc
     1081 agattccagc aaatcgggat cgtgcttgcg gatgacatcg cgcagcttgg ccagcgcgcg
     1141 atgcagccgc gatttgatcg tcccggcggc gacgtcgaaa gtctgcgcca gctcggcctc
     1201 ggacaagtcg aagaagaagc gcaggatgag caggctttga tcgtcgggct tgagatgacg
     1261 aatcgcgtcc tgcacgcgcc gtccccgctc gcggcgcagc acacgcgcct cgatcgtcgg
     1321 gttcgcttca cgctccacgg ttcctgctcc gaatcgttcg gccattgcct gccgccgcat
     1381 cgacgccttc acacggttga gcgccgcgtt cgtcgcgatg cgcaagatcc acgggcggaa
     1441 gggccggcgc gcgtcgaagc ggcccagcgc gtcgtaggcg cggataaagg cttcttgcgc
     1501 cgcgtcttgc gcgtcggcga cgtcacgcgt aatcatatag gcggcgcgga aggcggcttc
     1561 ctgatgcgcg cgcacaagct cgtcgtacgc gcccacatcg ccgcgccgcg cacgctcgat
     1621 caattggcgc tcgtccaacc acgttctccg gagaacggtg ggttctgctt atattacaac
     1681 ggtcgtcgaa aagaggttcc ccaggggaga gttagaggag ggagttagag tgcagagttt
     1741 gagggggcgt ggagcgtggg cgctccgtac ctgggcgtgc ctgactccgt acggactatg
     1801 cgaccacggg gcgaccagac ggcttcgcca ccggcaccgt gaacgcgaac gtcgagcctt
     1861 tgcctacccg gctctccacc catatctcgc cgccgtgcat ctcgatcagg ctcttggtga
     1921 tcgccaatcc cagcccggtg ccgggcatct cgcgcacggc cggatcgccc gcgcggaaga
     1981 acttggtgaa cagcttggcc tggtcctctt cggaaatgcc gacgcccgtg tcccgcacgg
     2041 cgcacaggac gaagccgggc gagccgcctg acctgggttg ggcgctgacg acgatgtgcc
     2101 cgccggccgg cgtatacttg tgagcattgc tgatcaagtt ggtcatgatc tggatcaggc
     2161 tggcgcgatc gcccatgacc atgggcaggt cccggggcac ggctatgtcg agcttctgct
     2221 gcctggcttc gatttggccc cgcgtggtgc gcagtgtctc ttcgatcacg tcttccatcg
     2281 acaccggctt gatgtcgagc ttcaaacgtc cggtctcgat acggctcagg tccagcagtt
     2341 tgttaacgag atcgctcatg cgatcgacgt tggagcgcac cgtgttgaga aactgctgct
     2401 gaacgtcggt caatgacccg gccgcgcctt tgatcatcag gtcggtgtag cccttgatgc
     2461 tcgtcatcgg cgtcttcagc tcgtgcgaca cggtcgacac gaactcgctc ttgtcgtcgt
     2521 tggcgcgctt gacttcctcg aacaggcgtg cgttggcgat ggccgtcgcg gcgtggtcgg
     2581 ccaggcggat cgcgctctcg aattctaccg tgctgaacgc acgcggctga aaagactcca
     2641 ggtttagcac gccgacgacc tcgctgccgc gccggatggg tacgctgatt tgagatcggg
     2701 tctcgggcga cgcgggcgcg ccctccgggt ccggcgtcgt gtcgtcgagc agcaagggca
     2761 gcccggtgcg gatcgacttc ccggcgagcc cccggtcgag cggcagcgct ttgccgtcgt
     2821 ccgacgcgtc ggcgccggaa taatcgcggc tggccaggat caagagcgca ttctgctctc
     2881 gatcgatcat gccgatcgac ccggcttctg cgccgctcac gcgcagtccc caatcgagtg
     2941 tcaggcgcat cacgcgcttg aagtcgagcg acgtgttcag ctcgcggtcg atctcctgca
     3001 gcgtgctcag ctcttcgacg cgccgcgcca gcgccaggtc ggtgcggttg aacagcttcg
     3061 cattttccac ggcgacggcg gcgttgccgg cgaacgactc cagcagggtc tggtcgtctt
     3121 cgctgaacgg cgtgccgtcg cgcttgttga gcacctggat cacgcccatg actttgccgc
     3181 gcgacttcat cggcacggtc agaagggcgc gcgtcatgaa ctggtcggtc tggccgacgt
     3241 cgcgcagcca gcgctcgtct cgctgtgctt cgttgacgat caagggacgg ccggattgcg
     3301 ccacggagcc gacgatgccg gcgccaggcg gcaggcgcgt gccggccaac tgatccttgg
     3361 acgagccgag aatgacctcg aagacaagct cctgggtcgt ttcgtcgacc agcagcagcg
     3421 aacccgcttc cgcgccgagg atctcgaccg ccttctccat gatccggttg agcacggtgc
     3481 gcagatcgag cgaggacgtg atgcttgccg agacttcgtt gagcaccgcc aactgttgcg
     3541 cgcgatcttc ggtgattcgg aacagccgga tcttctccag cacgctggcg gcttgatcgg
     3601 cgatggcaga gaagatttga agctgctccg cgccgtaggt cacgtctgga tcgaacgacg
     3661 agacgttcat gacgcccagt acctgatcgc cggcgttgag cggcacaccc atccaggcgc
     3721 ggccaggttt gccgccgggc gtgatgccgc gccggagaca ttcctgcacg tagtcggtgg
     3781 cgacgatcgg ctggcctgtc cggacgatcg cacccgtgag gccgacgtcg agcggccact
     3841 cgtcgtcggg tttcagccgc tcgccgttct cgacgtagaa cgcgaagcgc atcgtgttgc
     3901 gactcaagtc ggccagcgcg atatagaaat tggaagtgtc cagcaccttg ctcgcctgcg
     3961 cgtaaatcag ctccagggtg gcatccggat cgaccttgaa gttgactccc tggctgacct
     4021 ggctgagcgc gttcagctcc gtcacccgcc gctggagatc ggcgtgagtc atggcgcgct
     4081 cgagcgccag cgtcgtttga tcggccagcg cctcgaggaa gctcagatcg tcggtggtat
     4141 acggctgtcc cgacgccttg gcgcccagcg caacccaacc gttcaatcga tcgcgcccgt
     4201 gcaacgggac gacgagcgac gcgcccatcg cctccaggcg cgcgctctcg cccgcgatcg
     4261 gctcgggcag cggacgatcc ggctggatgt accacggctc atggcggtcg ctcagccaac
     4321 gcgccagcgg actgtcccac gggcaacgtg ccagcagcgg gggcggcttg cccgtctcac
     4381 cctgcgacgc gtgcagcagg taggatcgca cgcgcggatc gcccaggtac acgcgcagtg
     4441 gatcaggctt gagcgtctcg ccaatcgcgg cggccaacag cgatacgatc gaagcgagat
     4501 cggccgcgcc ggtgagatcg tgactgtacg tttgcagaag cccgtgcacg tcgatgcgga
     4561 cggggaagaa catgcgatcg acggcgagct gcacgcgctt gcgcagcgga tcgagcagca
     4621 cggcgacgat cagtacgaag acagccagca ggctgggatt cgacatcagt gcttgcgtgt
     4681 tctgcgccag cgcgccgatc accgcgatgg tgaggaaata ggcgcccacg acgagggcga
     4741 cgacgatcgc gtacaccagc gcccggttga ggatgagatc gacatcgagc agatggtagc
     4801 gcaccagcgc ataggcgatc gacagtggga aaaggacgag gggcgggaag tagatcagcg
     4861 gatcgaacgg cgcggtgacg cctactatgc cggcgatgat ccacaaggca aagggcgcaa
     4921 aagcgaccac cgagccaagc agtatgacgc ggctctgctg tcgcacgacg ggtgaagtgg
     4981 aacccagccg gcggtagacc agcataccgt agaagaccac caaacccagc gcgatgaaca
     5041 ggtacaacag ccgccaggca gtgatgtagt cccacggctg gctcagatcg ggctgtaccg
     5101 tgagtgtgta tgccaccagg gccgcgacgg gaacgtaggg cagcaggcgc agcacgggcc
     5161 gccgcaagac gaagcgcggc tcctggggaa atacgagcgc caggtgtatc atggcgccgc
     5221 cggataacgc gaggcccaac gcccacagca ccaagagcac gtgcgtcgtg ttcatatcga
     5281 agaaaacggc gagcgtgacg gcgacacagg cgctgaagaa ggtaaaggtt agcccggcgc
     5341 gctggtggcc tcggagacgg aaaacccaaa gcccgatgcc gagatacacc agcccgatca
     5401 ggtaaggcag ccagaacagc cgcaagaggt cgccctgcgg gaatgggccg aggctcgcag
     5461 tgattgagcc gggctgcaat gtgccatccg gggtgcgtct cagatatgcg atttgagcgc
     5521 ggcggcccgg gtcagccgcg acttctgcca gcgcccggtc gagagcagcc tggctgtcga
     5581 tcgtgcgacc gttgatcgac tcgacatggt cttgcacgtt cagcccgtca ggcgaaggcc
     5641 cccagcccgt gccccggctg tcgttgacgt tgaaagtctg ctccagcatg acgccgagga
     5701 aaaccggctg gcccgcccag cggtaggcca gcaagggggc ggcgacatac acggcggcgg
     5761 cggctatcag gctgaggagg acgaatcgac gcgagtaacg cgcggcgggc ctttcgctcg
     5821 aagcagttaa agcggctggg acgtctgcca ctgaaaaccc ctggcgtcaa gaactcatat
     5881 caacaccatt tgattctagc atggcgacat acgtttgtct accggacaat tccgttacgg
     5941 tttcgtttcg cggcttcacc ctctggcacg gtcaagcctg tcctgagcga agcctgccct
     6001 gagcgcaggc gaagggtcga aggaatggtt agcaagaagt tgtcctgaat gtgacaatct
     6061 tccccagccc ttgtgagtgt tgtataatcc cttgagatcg tgtcgatcga caataccggg
     6121 catgccgttt ccgcgacgag ccttcccgag gagcatggat ggatagaaaa attcgcgttt
     6181 tgatcgccaa gccgggattg gatgggcacg accggggcgc aaaggtgatc gcccgcgcgc
     6241 tgcgcgatgc gggcatggag gtgatttata cgggcttgcg ccagacgccg gagatggtcg
     6301 ccgaggcagc actgcaagag gacgtcgacg tcgtcgggct gtcgattcta tccggcgcgc
     6361 atatgacact cgtgccgcgc gtcgtcgatc tcctcaagca gaaggggctt gacgacgtgc
     6421 tcgtgttcgt cggcggcatc attcccgacg aagacgtggc gcctctcaag gccctcggcg
     6481 tccggggcat cttcggccct ggggcgaaca cgaccgaggt cgcgcgcttc atcaccgagc
     6541 aagtcgggtc tgcctgaaat gcgccaggcg cgacgctctc cacgcgaact cgtcgacggc
     6601 gcgctgaccg gcgaccgccg cgccctggcg cgcctgatca gcttgctcga gggcgattcg
     6661 caggcggcgg ccgagtgtat ggtccacctg catccgcgcg cgggtcgcgc agccatcgtc
     6721 ggcatcacgg gcgcgcccgg cacgggcaag agcaccctgg tcaacgagct ggcaaaggcg
     6781 ttccgccgca gaggccgaac cgtgggggtc atcgcggtcg atccgacgtc gccgttcagc
     6841 ggcggggcca tcctcggcga ccgcatccgt atgcgcgatc tgtccggtga cgacggcgtg
     6901 ttcatccgct cgatggccac gcgcggcagc ctgggtgggt tggcccgcgc gacggccgac
     6961 gtcgcctgca cgctggacgc ggccggcttc gacgtcatcc tgatcgaaac ggtgggcgcc
     7021 gggcagagcg aagtcgacat tgcgcgcacc gccgacacgg tgctcgtcgt cgaagcgccg
     7081 ggcctgggcg acgaagtgca ggcgatcaag gccggcatcc tcgaaatcgc cgatgtgctg
     7141 gtcgtgaaca aggcggatcg cgaaggcgcg gagaacacct ttcgcacgct gcaggcgatg
     7201 ctcgacctgg gagctgcgcc gcgcgcggtg cgccaccacg gccgggtgat ggaagtcccc
     7261 gattcttccc cggcagacga ccagccctcc ggctggcgcc cgccgatcgt caagacagtc
     7321 gccatagaag gcaaaggaac cgacgagctg atcgccgcta tcgatggtca ccaggcgcac
     7381 ttagaagcaa gcggcgaagg cgcggcgcgg cggcgccagc gcgccgaaac cgagcttgag
     7441 acgattctgc gcgaaacact tctgcagcgg ttgaggaagc aggtaggccg ggttggtctg
     7501 gacggcgtcg tcgatcgggt cgcatcacgc gagctcgatc catacacggc ggcgcgcacg
     7561 ctgatcgacg gctagaccca atactgttta ctggagtttg gcgacagccc tatctcgtgc
     7621 ttggagtgca ggcttcagcc tgcgaagcgc catcccgatg gagattgtcg ttgagcgtcc
     7681 ggctgtatct cgtccgtcat gccgaatcga cctggaacgc ggaagggcgt gtccagggtc
     7741 aggccgatcc tccgctcagt gagcgaggcc tggcgcaggc ggagcggctg gccgagcggt
     7801 ttcgcggcga gccgatcgtt gcgttgtact ccagcccttt ggaacgggcg cggcagacgg
     7861 cggaacgcat cgccgaggcg accgcgctgg cgacccagat tgacaatcgc ctgaaggaac
     7921 acgatatggg tctgttcacg ggcctggtgt ggcgagagat tgtgcaacag ttccccgaat
     7981 tcgcgcgggc ctggatggag cgcgccatgg atatgccggg tggagagagg caggcggcgt
     8041 ttcacatgcg cgcgacgggt gtcatgcagg acatcgcagc gcgacattcc gacggcaaga
     8101 tcgtcgtggt gtcgcatggc gggattctgg gcgagtacct ggcgcatctg ctcggactcg
     8161 atccggagcg gcggcctccg ttccgcttcg acaacgcatc ggtgagcgtg gtggaagtcg
     8221 gcggcccgct gcccgctcta cgcgtgcacc gtctgaacga tgtgagtcac ctgatcgcgc
     8281 cgtccggcga tggccgccgg atcatgggcg acgccgacga ggtggatgga tgagatcggt
     8341 gactcgggcg tacagcgata tccgcatctt cgccggctcg gggtcacggc acatggcgga
     8401 ggaggtctgc cggtatctca acgttccgct cagcccgtgc gaagtgatcc gcttccccaa
     8461 cgagaatatt tttgtccgcc tgcaggccag cgtgcgcggc caggacgtgt tcgtcattca
     8521 gggcacctcg tcgccggtaa gcgacaacat catggagctg ttgatcctgc tggacacggt
     8581 gaagcgagcc aacgccggcc gcatcacggc agttatcccc tatttcgcgt acggccgcag
     8641 cgacaagaaa gaccagcctc gcgtccccat cacggcgcgc ctcctggccg acatgatcac
     8701 cgccgcaggc gccgaccggt ttctggtgtt ggacttgcac gccggccaga tccagggctt
     8761 cttcagcatc ccgggcgacg agctgacgac ttttcacctg ctcagcgact acatgctcac
     8821 gaaggatctg agcaacgcag tcgtcgtgac ggccgacctg gggttcgcca agaagggccg
     8881 gaacttcgcg cacaagctca acctgcccat ggctttcgtc gagaaacggc gcacgggcaa
     8941 cgacgcccgc gcgcaggcgc tgtctgtgat cggcgacgtg cgcggtaagg acgtgatcat
     9001 cgtcgacgac gaggtcgata cggctggctc cgtgtcggac gccgtgcgca ttgtgaagga
     9061 gaacggcgcg gcggacgtgt atctctcctt cgtccacgcc gtgctgtccg gcccggcagt
     9121 cgatcggctg cgcgcgctgc ccatcaagga gattatcacg accaatacag tgcccattcc
     9181 cgaatcgaag cagctgccca atatgaagat cctgtccgtc gcgccgctgc taggcgaagt
     9241 gatgcggcgc attcacgagg ccgtgtcggt cggtcagatg ttcaacgagt gaccttttgc
     9301 tttcgtcccg cgccgatgta taatcgtcat atggcactca cgcgtgagca ggtcgaccac
     9361 atcgccgagc tggcgaagct cgcgctgacc gacgcggaga aagcgcgctt ccaggagcag
     9421 ctctccgaca tcctggacta tgcccagcgg ctccaggcac tgaacacgga cgccatcccg
     9481 cccacggcca acgtcctgcc gctgcgcagc gtcatgcgcg acgacgtcgc cgcgcccagc
     9541 accccgacgc ccgtattgct ggccaacgcg cccaaagcga gcggcgacgc gttcgaagtt
     9601 caggcggtgc tggactgagc gcccataccc caggaagttc cttcagttcc ttgagctcct
     9661 tacaaacctt gaaggaactg agggaactca gggaactcac ttgaaatgtc cctttacgcc
     9721 ttgaccatcc acgccgctcg cgaacgactt gcccgcaaag agatttcctc ggtcgagctg
     9781 acgcaggcga tgctggatcg cattgcagcc gtcgacggcc gcgtgcatgc ctttctgcaa
     9841 gtgtccgatc acatggcgct ggcccaggcg cacgaggccg acgcgcgccg tacccgaggc
     9901 gaagacacgc cactgctggg catcccgctg gcgatcaagg atgtgatctg cgtggaaggc
     9961 atgcgctgca cctgcggcag ccgcattctg gagagctttg tcccgttcta caacgcgacg
    10021 gttgtcgagc ggctgcgcgc gcagggcgcg gtgatactgg gcaagaccaa caccgacgag
    10081 ttcgccatgg ggtcgagcac cgagaactcg gccttcggcc cgacgcgcaa cccatggaac
    10141 ctggagcgcg tgccgggcgg ctcgagcggc ggcagtgcag cggcggtcgc cagcgagatg
    10201 gcgctcggcg cgctggggac ggacaccgga ggctcggtgc ggcagccggc cgcgctgtgc
    10261 ggcgtggtcg gcgtcaagcc gtcttatggc cgcgtcagcc gctatggcct cgtcgccttc
    10321 gcttcgtcgc tcgatcagat cggcgcgctc ggcaaagacg tgcgcgacgc ggcggacctg
    10381 ctcggcgcga tcgccgggca cgacccgcgc gattctacgt cgatgccggt cggcgtgtcg
    10441 gactacagcg cggcgctgaa actcgacctt aagggcgtga aagtcggcgt gccgaaagaa
    10501 tatttcatcg aaggcatgca gcccgatacc gagcaggcgg tgcgcgctgc gatcgacgtg
    10561 atgcgctcgc tcggcgcgga aatccgcgaa gtgtcgttgc ctcacacgga ctatggcctt
    10621 ccggtctatt atctcgtcgc gccggcggaa gcctcggcca acctggcgcg ttacgacggc
    10681 gtgcgttacg gcctgcgcct cagccctgcg ggcggacccg acgacggcct gtgggcgacc
    10741 taccgcgcga cgcgcggcgc gggcttcggc ccggaagtca aacggcgcat catgctcggc
    10801 acgtacgtct tgtcggcagg ctactacgat gcgtattact tgaaggcgca gaaagtgcgc
    10861 acgctgatca aggccgactt cgacgcggcc ttcaaagaag tagacgtgat cgcctgcccg
    10921 gtggccccta cgacggcctt caagatcggc gagaaggtgg acgatccgct tcagatgtat
    10981 ttgagcgatg tgttcacgct gaatgccaac ctggcgggca tctgcggcat ctcgctgccg
    11041 tgcggcttcg acgcgcaggg gcttcccatc ggcttgcaga tcaacggccc ggccttcggc
    11101 gaagagaaga tgctgcgcgt ggcgtacgcg tacgagcagg cgaccgagtg gcatcggcag
    11161 aggcctgcct tatgaacgac gaacaggacg ttggcggttg gatacagtat gcccaggccg
    11221 atcgacacgc tgcccaaagc ctgtttgcca gcggcgacta ctctgtgtgc gcgcagatgt
    11281 gccaacagat gctggaaaag atcatcaagg
//
LOCUS       DBNHXB010000122         8955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3714559, whole genome shotgun sequence.
ACCESSION   DBNHXB010000122 DBNHXB010000000
VERSION     DBNHXB010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8955
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3714559"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="AC162G_04705"
     CDS             complement(<1..146)
                     /locus_tag="AC162G_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018216771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5075148.1"
                     /translation="MDGSLAIVTQGLMKSYGRVWALRGVNLEVRRGEIFGFLGPNGAG
                     KTTT"
     gene            586..1269
                     /locus_tag="AC162G_04710"
     CDS             586..1269
                     /locus_tag="AC162G_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5075149.1"
                     /translation="MSDRRRQNGRSAATASRREREKARRREDILQAAREVFFERGIHH
                     ATVDEVAEQAQVSKGTVYLYFDTKETILAHLLLEGLDKLVGDLEAAFAPAESRTAPER
                     IRSMAKAYLKFYQSYPSYFNLISAFDRGRFQESISPELYAQVLMRSLRGLEWLVRAVE
                     QGRAEGTLRIDDARHVASVYWAGLNGVLVLLNHPLRREMVASDLESLYAAMTDVLLTA
                     YAAERIPPG"
     gene            1273..1581
                     /locus_tag="AC162G_04715"
     CDS             1273..1581
                     /locus_tag="AC162G_04715"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR5075150.1"
                     /translation="MEVRHSFKVMTYDIDFAGVVSNQVYIRWLEDLRNLLTDQVLSIG
                     DMMRRSIAPVLTRTEIDYRTPVRLPDTVDGRLWVEEVGRAKFVLGAEFVSRANGQSLP
                     "
     gene            1751..2965
                     /locus_tag="AC162G_04720"
     CDS             1751..2965
                     /locus_tag="AC162G_04720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR5075151.1"
                     /translation="MKDVVIVEGVRTPAGNHGGALRDVTAPVLAQTVIKGILERTKID
                     PASIDEVILGCIAQPSEAPNIARVAALMAGIPKEVPAYTLARNCDSGMDAIVNAYKNI
                     VVGDGDVYLVGGVESMSNIPYVIKGARWGLKLRHSQLTDAMWEGLTDPICGQLMGRTA
                     ENLVDEFEITRQEQDEYAVQSHKKAFMATRMGKFKEEIVPVEVTKKVAGQEVARELIT
                     QDETINPALSVQKAALYPAIFKEGGSVTPANACGISDGASAMLIMSAGKAQELGYSPM
                     ARIVSYGYAGVEPQRMGIAPAHAVPKALQRGGLTLRDMDLIELNEAFAAQVLSVGRAL
                     HDQGWDWSKVNVNGGAIALGHPVGSSGCRIVVTLVHEMKRRGSRYGLATLCAAGGQGS
                     ALIVERANGVSG"
     gene            3118..4107
                     /locus_tag="AC162G_04725"
     CDS             3118..4107
                     /locus_tag="AC162G_04725"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR5075152.1"
                     /translation="MSGINPNEVRFFSGSSNRPLAADIAAHLGVPLDKTRISRFSNDN
                     LYIQLSASCRSRAVFIVQSLSPPVNDHLVELLMMLDIARSAAAKEIHAIIPYFSFARS
                     DKKDAPRISITARLVADLLQTAGATHVMTMMLHSPQVHGFFGVPADPLTARPVFEDYF
                     RSRDLSDTIVVAPDVGHLKSAARFAESFKLPVAAGNKERVSDTQVVISGLVGHQVCGF
                     RRAMIFDDEIATGSSVLELCRVLLDNDVKEIWAVCTHGVFTGRALERLAATPQITEIV
                     ATDTVHIPPEKRPPKLHVVSVASIFGEAIRCNYLHKSIGDLFVYGVPDAEREQ"
     gene            4240..5454
                     /locus_tag="AC162G_04730"
     CDS             4240..5454
                     /locus_tag="AC162G_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HIR5075153.1"
                     /translation="MYIFKAAVVGAGAMGAGIAQVISYSGLPVVLKDVDQGMLDKGMS
                     HVRKVYQGRVDKGKMSASELESRMALVTPALDYGEFTDVDIVIEAVPERMDLKKRVFA
                     ELDAACPEGAIFASNTSALSISEMGRATKRPHKMIGMHFFNPAHVMKLVEIIRGEGTD
                     EETVQDVVTFTESLRKIPVVVKECPGFLVNRLLTPYLNEATLALQEGAATAQEIDGAM
                     VDYGWPMGPFTLMDFLGLDVCHDVGKYLSSQYGERMQGAILFEKLVKAGRLGEKSGAG
                     FYGYGDQTDEPVKQMIAEISGGPKLSEFSPERLMYLLLNEAARCVEEDIADVRDIDMA
                     MIAGTGMMVKGERIGPLALADRIGIDVLVASLEDFEKKYGRRFHPAEKLYELLKEGRT
                     GEKARGGFLEYV"
     gene            5677..6465
                     /locus_tag="AC162G_04735"
     CDS             5677..6465
                     /locus_tag="AC162G_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR5075154.1"
                     /translation="MADFIRYTVEDCVATLVIDHPPVNALNSQTLMELNTAIDELLAD
                     DEVKVIVVTGGGQLAFVAGADIGEIGSIVKSRDGRAAVEMIELGQCVFTKIERSHKPV
                     IAAINGVCLGGGLELAMACHMRIAGDRVRLGSPEINLGIIPGWGATQRLPRIVGAGKA
                     TEMILTGDQITAQQALQLRLVNLVVPGGEVMRQAKGLARKIAEKSAVSVSAALTAIGA
                     GLDGDLQAGFAKEREQFARIADSEDVREGVSAFLEKRKPQFKDR"
     gene            6551..7714
                     /locus_tag="AC162G_04740"
     CDS             6551..7714
                     /locus_tag="AC162G_04740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5075155.1"
                     /translation="MDFSLSEEQQMFRKMFDEFVRKEVARVAEHTDKAEEPPMDVLRK
                     AAAQGFLGGPIPEAYGGAGLDHVSYAMLIESIARECLSTAIIIGMHTSLVGETILEFG
                     TEEQKERWLPALASGESIGAFAMTEPDAGSDPSRIATRALKEGDAYVLHGVKTWVSNG
                     AIAGLLLVIASSDPSANGKGLSAFVVAADAPGLKIGRREPTMGLRGLPVNTVYFDGVR
                     VPESDRLGEEGHGLSIGLRAVNYMRLGLGAAALGSAEGALELGKKFAVERKQFGVPIG
                     SKQALGNYFADSAADIEALRWLVIRAAWLADHMQDNRDAVSMCKLIGARVARDVANRM
                     LQVHGGYGFSDEYAISKVYRDTRALDLLGGTGQIQRVVLWQSVFEGTEVHITP"
     gene            7763..8929
                     /locus_tag="AC162G_04745"
     CDS             7763..8929
                     /locus_tag="AC162G_04745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5075156.1"
                     /translation="MDFELSREHRALRKLVREFAEDEIRPISKRADRHSQPPMETIRK
                     AAAIGLLGVPIPREYGGLGAGETGYCILMEELSRADTSLATIIGAHIGLAETSIYLGG
                     DKRIREKYLPDMCAGKKIGAFALTEANAGSDAASIKLKATREGDSYILDGEKMWITNG
                     PVADVVSILAVTDPALGARGGATAFVVETEWDGFTAGKYEHKMGLHGSPTSAMTFDNL
                     RVPQENVLGQVGLGFVVFMQTLDIGRVSLGAATLGGAQAALQASLEWAKVRQQFGRSI
                     AHMQSIQFMIADMAAEIESLRSLVYRTAWLVDNNKPFIKEAGMCKLLGSEIGSRCIDR
                     AVQIHGALGYSRDSDVERGYRDARITEIFEGTNEIQRILIAEQVFREVGVRIRP"
BASE COUNT         1751 a         2612 c         3030 g         1562 t
ORIGIN      
        1 atcgtcgtcg tcttgcctgc gccgttcggg ccgagaaagc cgaaaatctc gccgcgcctg
       61 acttcgaggt tgacgccgcg cagcgcccac acgcggccat atgatttcat cagtccttgc
      121 gtaacaatag cgagggagcc atccatgcgc agtcctttcg tcacggaaag cacaagcaat
      181 cgtttgctgt tgccatcagg ccttggcgca ggtcatattc tacttcttta tcgacgattc
      241 gtctaacctg acaatatcag attcagctcg ccccgccgtc ccttcgtcag gctccccact
      301 ccctacggtc ggggacttcg cagggcaggc cccggcgcgg tcaacgtcga acttccgcct
      361 gaaggctcga ctccgggatg ccgtaaacca gactggaaac ggctataaat gcggcattgt
      421 cgaagtaatt cggttctcgc gggggcctgc atcgtgacct cagggccgac cagggtgaat
      481 gtcgtgcaac aggtagaatt cggatgaaat gcttgacatt caacgaaata aggaatatac
      541 tgactattgg tcagtatttg actgccagtt ctctacggag agaaaatgtc cgacaggcga
      601 cgccagaacg gtcgatctgc ggcgaccgcc tcacggcgcg agcgcgagaa ggctcggcgg
      661 cgggaagaca tcttgcaggc ggcgcgcgag gtgttcttcg agcgtggcat ccaccacgcg
      721 acggtcgacg aagtcgccga gcaggcccag gtgagcaagg gcacggtcta cctgtatttc
      781 gatacgaagg agaccatcct tgctcatctg cttttagagg gcctggacaa gctcgtcggc
      841 gatctcgaag cggcgttcgc gcccgccgag tcgcgcactg cgcccgagcg catccgaagc
      901 atggccaagg cttatctcaa gttttaccag tcctaccctt cgtacttcaa tctcatttcg
      961 gcttttgatc ggggccggtt ccaagaatcc atctctcccg aactctacgc gcaggtgctg
     1021 atgcgcagcc tgcgtgggtt ggagtggctg gtgcgcgcgg tagagcaggg gcgcgccgaa
     1081 ggcacgctgc gcatcgacga tgcgcgccac gtggcgagcg tgtactgggc gggcctgaac
     1141 ggcgtcctgg tcctgctcaa ccatccgctg cgccgcgaaa tggtcgcgtc cgatctcgaa
     1201 tcgctgtatg cggcgatgac cgacgttttg cttacggcgt acgcggccga gcgtattccg
     1261 ccaggttagg tcatggaagt tcgccattcg ttcaaggtga tgacgtacga catcgacttc
     1321 gcgggcgtgg tcagcaacca ggtctacatc cgctggctgg aggatttgcg gaatttgctt
     1381 accgatcagg tgttgtcgat cggcgacatg atgcggcgaa gtatagcgcc ggtgctgacg
     1441 cgcaccgaaa ttgattatcg caccccggtc cgtttacccg ataccgtcga tggtcgcttg
     1501 tgggtagagg aagtggggcg ggcgaagttc gtgctcgggg ccgaatttgt cagccgggcg
     1561 aacgggcaga gcttgccctg agcttgccga agggtcgccg ccacggccag gcagttcggc
     1621 gtgtttgtct cgacgcagac gagccggccg gtgcccctgc ccgaagaata cagagttcat
     1681 tcgtgatcct gtaacaatac gcgatatgca ataggcgata agcgataagc caccagcaag
     1741 gaggatcgat atgaaagatg tcgtgattgt ggaaggcgtg cggacgcctg ccggaaacca
     1801 cggcggtgcg ctgcgagatg ttaccgcgcc cgtcctggcg cagacggtga tcaaaggcat
     1861 acttgagcgc acgaagatcg atccggcgtc gatcgacgag gtgattctcg gatgcattgc
     1921 ccagccgtct gaagcgccga acatcgcccg cgtggccgcg ctcatggccg gcattcccaa
     1981 ggaagtgccc gcttacaccc tcgctcgaaa ctgcgacagc ggaatggacg ccatcgtcaa
     2041 cgcctacaag aacatcgtcg tcggcgacgg cgacgtctac ctggtcggcg gcgtcgagtc
     2101 gatgagcaac attccgtacg tcatcaaggg cgctcgctgg ggcttgaagc tgcgccactc
     2161 acaattgacc gacgcaatgt gggaagggct gaccgacccg atctgcggcc aactgatggg
     2221 ccgcacggcc gagaacctgg tcgacgagtt cgagatcacg cggcaggagc aggacgagta
     2281 cgcggtgcag tcgcacaaga aagcgttcat ggcgacgcgc atggggaaat tcaaggaaga
     2341 gatcgtcccg gtcgaggtga cgaagaaggt cgccgggcaa gaagtcgcgc gcgagctgat
     2401 cacgcaggat gagacgatca acccggccct gtccgtgcag aaggcggcgc tctacccggc
     2461 catcttcaag gaaggcggct cggtgacgcc cgccaacgcc tgcggcatca gcgacggcgc
     2521 gagcgcgatg ctgatcatgt cggccggcaa ggcgcaggag ctgggctatt cgccgatggc
     2581 gcgcatcgtc tcctacggct acgccggcgt cgagccgcaa cgcatgggca tcgcgcccgc
     2641 gcacgccgtg ccgaaagcgc tgcagcgggg cggcctgacg ttgagggaca tggacctgat
     2701 tgagctgaac gaggcttttg cggcgcaggt gctatcggtc ggccgcgcgc tgcacgacca
     2761 gggctgggac tggagcaagg tcaacgtcaa cggcggcgcg atcgcgctcg gccacccggt
     2821 cggatcgagc ggatgccgca tcgtcgtgac gctcgtccac gaaatgaagc gacgcggctc
     2881 gcgctacggt ctggcgacgc tgtgcgcagc cggcgggcaa ggcagcgcct tgatcgtgga
     2941 gcgggctaac ggcgtcagcg gataggcaac ggataaacgg atgcggtgcg agtcttacga
     3001 tatgcgataa gcgacaggcg ataggcgata tgctgcggcg tacagcgata tgccatctgc
     3061 gataagtaat aagcgacagg cgacatgtca tcagccatca gccgtctgcc atctgccatg
     3121 agcggcataa acccgaacga agttcgattc ttcagcggca gctcgaaccg gccactggct
     3181 gccgacatcg cggctcatct cggcgtgccg ctggacaaga cgcgcatttc gcggttcagc
     3241 aacgacaatc tgtatatcca gcttagcgcc agctgccgct cgcgcgcggt gttcatcgtg
     3301 cagtcgctct cgccgccggt gaacgatcac ctggtggagc tgctgatgat gctcgacatc
     3361 gcgcgcagcg ccgccgcgaa ggagatccac gccatcatcc cgtatttctc gttcgcgcgc
     3421 tcggacaaga aagacgcgcc gcgcatctcg atcacggcgc gcctcgtcgc cgatttgctg
     3481 cagacggcgg gcgcgacgca cgtgatgacg atgatgctgc actcgccgca ggtgcacggc
     3541 ttcttcggcg taccggccga cccgctgacc gcgcgtcccg tgttcgaaga ctactttcgc
     3601 tcgcgtgatc tgtcggacac catcgtcgtc gcgccggacg tcgggcattt gaagtcggcg
     3661 gcgcgtttcg cggagagttt caaattgccc gtcgccgccg gaaacaagga gcgcgtgtcg
     3721 gatacccagg tggtgatcag cgggctggtc gggcatcaag tgtgcggctt ccggcgggcc
     3781 atgattttcg acgacgagat cgcgacgggc agctcggtgc tcgaactgtg ccgtgtgctg
     3841 ctggacaacg acgtgaaaga gatctgggcg gtgtgcacgc acggcgtgtt caccgggcgg
     3901 gcgctcgagc gcctggcggc gacgccgcag atcaccgaga tcgtcgcgac tgacacggtg
     3961 cacatcccgc cggagaaacg cccgccgaag ctgcacgtcg tttcagtcgc atcgatcttt
     4021 ggcgaggcga tccggtgcaa ctacctgcac aaatcgatcg gcgacctgtt cgtatatggc
     4081 gtccccgacg ccgagcgtga gcagtgaacg gcgctcaggg acggctcgta gcgggtctgc
     4141 ggcccgcgcc ttgtccggtt tgagccgctg gaccacagac ccagcgggag cggaatgcga
     4201 taagccatac gcgatatgcc ataagcgaag gagcttgcta tgtacatctt caaagccgcc
     4261 gtcgtcggcg caggcgcaat gggggccggc attgcccagg tgatttccta ctccggcctg
     4321 ccggtcgtcc tgaaggacgt cgatcaaggc atgctggaca aaggcatgag ccacgtgcgc
     4381 aaggtgtatc agggccgcgt cgacaagggc aagatgagtg cgagcgagct ggagtcgagg
     4441 atggcgctcg tcacgccggc gctcgactat ggcgagttca cggacgtgga tatcgtgatc
     4501 gaggccgtcc cggagcggat ggacctcaag aagcgggtgt tcgccgagct ggacgctgcc
     4561 tgtcctgaag gcgcgatctt cgccagcaac acttcggcgc tgtcgatcag cgagatgggt
     4621 cgagcgacga agcgcccgca caagatgatc ggcatgcact tcttcaaccc ggcgcacgtc
     4681 atgaagctgg tcgagatcat ccgcggcgag gggacggacg aagagaccgt gcaagacgtc
     4741 gtcacgttca ccgagtcgct gcgcaagatc cccgtcgtcg tcaaggagtg ccccggcttc
     4801 ctggtcaacc gcctgctcac gccgtacctc aacgaggcga cgcttgcgct gcaggaaggc
     4861 gcggcgacgg cgcaggagat cgacggcgcg atggtcgact acggctggcc gatggggccg
     4921 ttcacgctca tggacttcct cggcctggac gtgtgccacg acgtcggcaa gtacctgagc
     4981 agccagtacg gggagcgcat gcagggtgcg atcctgttcg agaagctcgt caaagccggc
     5041 cgcctgggcg aaaagagcgg cgccggattc tacggctacg gcgatcagac tgacgagccg
     5101 gtgaagcaga tgatcgccga gatcagcggc ggaccgaaac tgagcgagtt cagcccggag
     5161 cgcctgatgt atctcctgct caacgaggca gcccgctgcg tcgaggaaga catcgccgac
     5221 gtgcgcgaca tcgacatggc gatgatcgcc ggaactggca tgatggtgaa gggcgagcgc
     5281 atcggaccgc tggcgctggc cgaccggatc ggcattgatg ttttggtcgc cagcctcgag
     5341 gatttcgaga agaagtacgg caggcgattc catccggcag agaaactgta tgagttgctc
     5401 aaagagggcc ggacaggtga gaaggcgcgg ggtggtttcc tggaatatgt atgaagcgtg
     5461 gagcgtgaag cgtgatgcgt aaaacgtgag gcgtaaaacg tgaagcgtgg aacgtgatgc
     5521 gtgagggtgt gatgcgtgag ggcgtgatta cgtgattacg tgattcgtga tggcggagct
     5581 cttggcttca cctcgacaac aaagagcggc aggcgatacg cgataagcca tcagccatct
     5641 gccatccgcc atccgctatc cgcaagagga gagaacatgg ctgatttcat tcgatacacg
     5701 gtcgaagact gcgtagcaac gttggtgatc gatcatccac cggtcaacgc gctgaactcg
     5761 cagacgctga tggagctgaa cacggcgatc gacgagctgc tggctgacga cgaggtgaag
     5821 gtcatcgtcg tcacgggcgg cggccaactg gcgttcgtcg ccggcgccga catcggcgag
     5881 atcggctcga tcgtaaaatc ccgcgatggg cgcgcagcag tggagatgat cgagctgggc
     5941 cagtgtgtgt tcaccaagat cgagcgatcg cacaaaccgg tcattgctgc gatcaacggc
     6001 gtgtgcctgg gcggcgggct ggagctggcg atggcctgcc acatgcgcat cgccggcgat
     6061 cgcgtgcggc tcggatcgcc ggagatcaac ctgggaatca tcccgggttg gggcgccaca
     6121 cagcggctgc cgcgcatcgt cggtgcgggc aaggcgaccg agatgatttt gaccggcgat
     6181 cagatcacgg cgcagcaggc gctgcaactg cgcctggtca acctcgtcgt gccgggcggg
     6241 gaggtgatgc gccaggcgaa aggcctcgct cgcaagatcg ccgagaagag cgccgtatcg
     6301 gtgtcggcgg cgctcactgc gatcggcgca ggcctcgacg gcgatctgca ggccggcttt
     6361 gcgaaggagc gagagcagtt cgcgcgtatc gccgacagcg aagacgtgcg cgagggcgtc
     6421 agcgcgtttc tcgagaagcg aaagccgcag tttaaggata ggtaacctca ccaccctctg
     6481 accccctccc tctcctgacg ctgcgtcagg agagggaggg gaaggggggc gggtgaggcg
     6541 ggagttggct atggacttct cattgagcga agaacagcag atgttccgca agatgttcga
     6601 cgagttcgtc cgcaaggaag tggcgagagt cgctgagcac accgacaagg ccgaagagcc
     6661 gccgatggac gtgctgcgca aggccgccgc acagggattc ctcggcgggc cgatacccga
     6721 agcgtacggc ggcgcgggcc tcgaccacgt ctcgtatgcc atgctcatcg aatcgatcgc
     6781 cagggagtgc ctgtcgacgg cgatcatcat cggcatgcac acgagcctgg tcggcgagac
     6841 gattctcgag ttcgggacgg aggagcagaa agagcgctgg ctgcctgcgc tggcgtcggg
     6901 cgagtcgatc ggcgccttcg ccatgacgga gccagatgca ggctccgacc cgtcccgcat
     6961 cgcgacccgc gcgctgaaag aaggcgacgc ttacgtgttg cacggagtga agacctgggt
     7021 gagcaacggc gcgatcgccg gcctgctgtt agtcatcgcg tcgagcgacc cctcggcgaa
     7081 cggcaaaggc ctgtcggcat tcgtcgtcgc tgcagatgcg cccggcctga agatcggccg
     7141 ccgagagccg acgatgggat tgcgtggcct gccggtcaat acagtctact tcgacggcgt
     7201 gcgtgtgccc gaatcggatc gattgggtga ggagggacat ggcctgtcga tcggactgcg
     7261 ggccgtcaat tacatgcggc tcgggctggg cgcggcggcg ctcggcagcg cggaaggcgc
     7321 gctggagctg ggcaagaagt tcgccgtcga gcgcaagcag ttcggtgtgc cgatcggcag
     7381 taagcaggcg ctggggaact atttcgccga ttcggccgcc gatatcgagg cgctgcgctg
     7441 gctggtgata cgtgcggcgt ggcttgcgga tcacatgcag gacaaccgcg acgcggtctc
     7501 gatgtgcaag ctgatcggcg cgcgcgtcgc gcgagatgtc gccaatcgca tgctgcaagt
     7561 gcacggcggc tacggcttca gcgatgagta cgccatctcg aaggtgtacc gggacacgcg
     7621 tgcgctcgat ctactgggcg gcacagggca gattcagcgt gtggtgctgt ggcagagcgt
     7681 cttcgaaggc acggaagtgc acatcacgcc atgagtcact tcccgcctgg cgtgtattga
     7741 tgaagcggag aggacgtgaa ctatggactt tgaactatcg cgtgagcatc gggcgctgcg
     7801 caagctcgtg cgcgagttcg ccgaagacga gatcagaccc atctccaagc gggccgatcg
     7861 ccacagccaa ccgcctatgg agacgatcag gaaagccgcc gcgatcggcc tgctgggcgt
     7921 gccgatcccg cgcgagtacg gcgggctggg tgcgggcgag accggttact gcatcctgat
     7981 ggaagagctg agccgcgccg ataccagcct ggccacgatc atcggcgcgc acatcggcct
     8041 ggccgaaacc tcgatctacc tgggaggcga caagcgcatt cgcgagaagt acctgcccga
     8101 tatgtgcgcc gggaagaaga tcggggcctt cgcgcttacc gaggcgaatg cgggctccga
     8161 tgccgcttcg atcaagctca aggcgacgcg cgaaggcgac tcgtacatcc tcgacggcga
     8221 gaagatgtgg atcaccaacg gcccggtcgc ggacgtggtc agcatcctgg ccgtcacgga
     8281 cccggcgctc ggcgcgcgcg gcggcgccac ggccttcgtc gtcgagacgg agtgggacgg
     8341 attcacggcg ggcaagtacg agcacaagat gggtctgcac ggctcgccga cgtcggcgat
     8401 gaccttcgac aatttgcgcg tgccgcaaga gaacgtgctg ggccaggtgg gactgggctt
     8461 cgtcgtgttc atgcagacgc tcgacatcgg ccgcgtgtcg ctgggcgcgg cgacgctggg
     8521 cggcgcgcag gcggcgctgc aggcgtcgct ggaatgggcc aaggtgcgcc agcagttcgg
     8581 ccggtccatc gcccacatgc agagcatcca gttcatgatc gccgacatgg ccgccgagat
     8641 cgagtcgctg cgctcgctgg tgtatcgcac ggcgtggctg gtcgacaaca acaagccgtt
     8701 catcaaggaa gctggtatgt gcaagttgtt ggggtcggag atcggctcac gctgcatcga
     8761 tcgggccgtg cagattcacg gcgcattggg ctacagccgc gattcggacg tcgagcgtgg
     8821 atatcgcgat gcgcgaatca ccgagatctt cgaaggcacc aatgagattc agcgcatcct
     8881 gatcgccgag caggtgttcc gtgaggtggg agtgaggata agaccgtgat cagttatcag
     8941 tgaccagtaa tcagt
//
LOCUS       DBNHXB010000123         3603 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_527450, whole genome shotgun sequence.
ACCESSION   DBNHXB010000123 DBNHXB010000000
VERSION     DBNHXB010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_527450"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="AC162G_04750"
     CDS             <1..1497
                     /locus_tag="AC162G_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075157.1"
                     /translation="LSKTGATERDTLSKTHAWVHPITLSYVPGALSPALERATEALQE
                     WLWAAGCTIESVPTNATDLIITTARFGTPVERDEALFFQAKRRYGLSRRPRLLTIVDV
                     PEVDYQRWLDHFVGLARQPDGVIPEHPYPGLGPHAVEVLARQAKRGGPEVAMGRFLQA
                     QAKSIQLMALRSDGAGRPLCAAHYDLAGAHPVTEAADLDAFAEDAGYRLLAAVCAEEV
                     NGHVFLPDPLPIAIWDSLVTPDAMIRAGRAFTEFGFFTEPVYVEKLLGFRGVSDAISA
                     QYSEGCYAAFDPDVLGLVMTASGSSRLVDKRSIGRDDQAIVVGIKPERDGAIVRRVKG
                     MDTIVPSVEAVEMMRICEAVTRRRWRNSRGEEVTVPAIRAILHGHIGVAAYNPERVEG
                     LSLDALYHAHLVSCGTNRLADATAAAFAHSVALRDPADPRRVVFLEQPGHGLVVVEKW
                     SEGKSAFDSIYECVAEGHLRVAMQVPQGAVQWEALTAPDGRRMMEKVE"
     gene            complement(1582..2568)
                     /locus_tag="AC162G_04755"
                     /pseudo
     CDS             complement(1582..2568)
                     /locus_tag="AC162G_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014476.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
     gene            complement(2625..3347)
                     /locus_tag="AC162G_04760"
     CDS             complement(2625..3347)
                     /locus_tag="AC162G_04760"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR5075158.1"
                     /translation="MANFKVPIVYGESFGGRPLAAYRLGHGPVRRALIGGLHGGYEWN
                     TTVLMSKTLEYLAASPDSIPEELTLYIIPLANPDGAAAGTDRVAGRMNGNGVDLNRNW
                     DYRWQITATHGVHPVYAGAFPFSEPETAALRDFILGRGISAAIFYHSAAAEVYSGAGR
                     DTSGTVALAKALAAKTGYRYAPEGIPGQITTGNAIDWLTTQGITAVEIELPTHASLDW
                     EPNLKAILAFMSWSLSSTLTAE"
     gene            3406..>3603
                     /locus_tag="AC162G_04765"
     CDS             3406..>3603
                     /locus_tag="AC162G_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075159.1"
                     /translation="MGTLCDCAGWEAGVGLAEIGIRRTIGAGVLARLAQPAAHRAIRK
                     IARMVLGIGALYFRANDAVNTK"
BASE COUNT          625 a         1096 c         1218 g          664 t
ORIGIN      
        1 ctatccaaga ctggagcgac cgagagggat acgctatcga agacgcacgc ctgggtacat
       61 ccgatcacgt tatcctatgt gcccggcgcg ctgtcacctg ccctggagcg ggcaacagaa
      121 gcgctgcagg agtggctgtg ggcagccggc tgcacgatcg aatccgtacc cacgaacgcg
      181 accgacctca tcatcaccac ggcccgcttc ggcacgcccg tcgagcggga cgaggcgcta
      241 ttctttcagg cgaagcgccg ctatggcttg agccgccgcc cacggctttt gaccatcgtc
      301 gacgtgcctg aggtcgatta tcagaggtgg ctggatcatt ttgtcgggct ggcgcggcag
      361 cctgacggcg tgattccgga gcatccgtat cccggcctcg gaccacacgc cgtcgaggtc
      421 ttggctcgac aggcgaagcg aggcggccct gaagtggcga tgggccgttt tctacaggcg
      481 caggccaagt ccatccagtt gatggcgctg cgcagcgatg gggccggacg acccctatgc
      541 gccgcgcatt acgatctggc cggcgcgcat cctgtgaccg aagctgccga cttggacgca
      601 tttgccgaag atgccggcta tcggctgctg gcggctgtct gcgccgagga ggtcaatggt
      661 cacgtttttc tccccgatcc actcccgata gcgatctggg acagcctcgt cacgcccgat
      721 gcgatgattc gcgcggggcg cgccttcacc gagttcgggt tcttcacaga gccggtgtac
      781 gtcgagaagc tattgggctt ccgaggcgtc agcgacgcga tcagcgcgca gtacagcgaa
      841 gggtgctacg cggcgttcga cccggatgtt cttggcctgg tgatgacggc ctccggcagc
      901 tcgcggctgg tcgacaagcg ctcgatcggc cgtgacgatc aggcgatcgt cgtcgggatc
      961 aagccggagc gcgacggcgc gatcgtccgc agagtgaaag gaatggatac tatcgtgccg
     1021 tcggtagaag ccgtcgagat gatgagaatc tgcgaagcgg ttaccagacg acggtggagg
     1081 aacagccgcg gagaagaagt gactgttccg gccattcggg cgattctaca cgggcacatc
     1141 ggcgtcgccg cgtacaatcc cgagcgcgtg gaaggcttga gtctcgacgc gctgtatcac
     1201 gcgcatctgg tgtcgtgcgg cacgaatcga cttgcggacg cgacggcggc ggcgtttgcg
     1261 cactccgtcg ccctgcgcga tccggccgat ccgcgcaggg tggtctttct ggaacagcct
     1321 ggccacggcc tcgtcgtcgt cgagaagtgg tcggagggca aatcggcgtt cgactcgata
     1381 tacgagtgtg tggccgaggg tcatctgcgc gtcgctatgc aagtgccgca gggagccgtg
     1441 cagtgggaag cactcacggc gcctgacggg cggcgcatga tggagaaggt cgagtgaaac
     1501 aaatgaccgg caccttgaca cctggcactt gacacggtgc cggtcactcg tagaacctga
     1561 cacctatgca cctgacaccc cctatagatt gaccaccgcg ccgagtttct tctgcatggc
     1621 ggccgtcagg gcttgctgcg cggcggccac gtcctgcaca gccagcccga ccgatttgaa
     1681 cagcgtgatc tgctccacgg ttatgcggcc ggggcgcgcg ccgttgacga cctcgcccag
     1741 ctcggtgagg tcgctctctg tcagggagcc gtccttgacg cagtcgatca cttcgccggc
     1801 ttcggccagc gccgcctgcc gctgatcgac gaacaccgtc gaacctcgca ccagatccgc
     1861 atcgatttcc cgcatcgccg gcgtgtacga gcctatggcg ttgatatgcg tgccgggccg
     1921 cacgtcggct gcccagagaa ccggcgtcgt ggagctagtg gccgtgcaca cgacgtcggc
     1981 ggccaggcaa gcttcctgcg cagacgccga gacttcgatc gacgcgggcg cgccgtcccg
     2041 gccggccatg tctgcggcga actgctcggc gtgagcgcgc gtacgtgagt ggacgaacac
     2101 acgctcgatg ttcctgacac agcacacggc ttcgagctgc gtgcgcgctt gagcgcccga
     2161 gccgaggatg gcgagcgtcg acgcatcccg gcgggcgagc aggtcggtgg ccaggccact
     2221 ggctgcgccg gtgcgaatgg ccgtcaacgc cgtcccttcg agcagcgcgg cgggctggcc
     2281 cgtcgccgcg ttgagctaga tcacgacggc gtggatcgtc ggcaggtcga ggctgcggtt
     2341 gttggggaag actgagacca gctttgcgcc gagccggttc ccgacgtgca cgtaggcggg
     2401 catgacgagt gtggcggcgt cgtgcttggg tatctcgatc gccgtgcgct gcggcaggtc
     2461 gacttcgccc tcggccagca ggccgaaggc tttgcgcatg gcgtcgatcg cgtcgcgcat
     2521 gggcagagcg gtgcgaacat cttgggcggt gaggatgtgg aagcgcatga aagcaccttt
     2581 cgatttaggg taattggtaa ttggtacagt cgccgccggt cgtgctattc ggccgtcagt
     2641 gtactggaca agctccacga catgaacgcc agtatcgcct tcaggttcgg ttcccagtcg
     2701 aggctcgcat gcgtcggcag ctcgatctct acggccgtga tgccctgcgt cgtcagccaa
     2761 tcgatcgcat tgccggttgt gatctggcct ggaatgcctt cgggcgcata gcggtacccg
     2821 gtcttggcag cgagcgcttt cgcaagcgcg acagttcccg acgtatccct tcctgctccg
     2881 gagtatactt cggccgcggc gctgtggtag aagatggccg cgctgatgcc gcgtccgagg
     2941 atgaagtcgc gcagggcggc ggtttccggt tcggagaatg gaaacgcgcc ggcgtaaacg
     3001 ggatggacgc cgtgcgtcgc ggtaatctgc caccgataat cccagttacg attgaggtcg
     3061 acgccgttgc cgttcatccg gcctgcgacg cggtcggttc cagcagccgc accgtccgga
     3121 ttcgccaggg gaatgatata gagtgtcaat tcctctggga tcgagtcggg cgaggcggcc
     3181 aggtactcca gcgtcttgct catcaagacg gtcgtgttcc actcgtagcc gccgtgcaag
     3241 cctccgatca gtgcacgcct gaccggacca tgtcccaatc gataggccgc aagtggccgt
     3301 cctccgaatg actcgccgta gacgatcggt accttgaagt tcgccacggg ggttgcgctg
     3361 acagtggaag aacgcgtcgt agctaatgct ggacgagaca aacgattggg cacattgtgc
     3421 gactgcgccg gctgggaggc aggagtcggg cttgccgaga taggaatccg gcgcacgatc
     3481 ggagccggtg ttctggccag gctggcgcag ccggctgcgc acagggcgat aaggaagatc
     3541 gcgcggatgg tcctcggcat tggcgcattg tacttccgag caaacgatgc tgtcaatacg
     3601 aaa
//
LOCUS       DBNHXB010000124        10461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_24656, whole genome shotgun sequence.
ACCESSION   DBNHXB010000124 DBNHXB010000000
VERSION     DBNHXB010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10461
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_24656"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="AC162G_04770"
     CDS             complement(<1..662)
                     /locus_tag="AC162G_04770"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019057045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR5075160.1"
                     /translation="MKVLLIGVGGVGEAIAVTAQCRPWLEKMVLADYDVARVEEVRAK
                     LGETTASKYPPEWIDAGDKERIVALARKHGVDLILNAVDPVFDEPIFEAAYETGCTYL
                     DMAMTLSRPHPDQPYRQAGVKLGDYQFEQSEKWEKKGLLALVGMGVEPGLSDVFARYA
                     EKHLFDEIDEIGVRDGSNLEVRGYAFAPTFSIWTTIEECLNPPVIWEDGRGWYTTEPF
                     SEP"
     gene            1246..3126
                     /gene="dnaK"
                     /locus_tag="AC162G_04775"
     CDS             1246..3126
                     /gene="dnaK"
                     /locus_tag="AC162G_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR5075161.1"
                     /translation="MGKIIGIDLGTTNSVAAVLEGGDPVVITTAEGSRLLPSVVAFAK
                     NGERLVGQTAKRQSVINPENTIFSIKRFMGKREQDVRHEKVPYRVVAGPSGDARVHVP
                     QAGKDFTPQEISAMILQKLKKDAEAYLGEPVSQAVITVPAYFNDSQRQATKDAGKIAG
                     LEVLRIINEPTAAALAYGLDKKKDETILVFDLGGGTFDVSLLEVGEGVVEVKATSGDT
                     HLGGDDWDQVLVDYVADEFKREQGIDLRQDRQALQRLREAAEKSKIELSSGMETEINL
                     PFITADASGPKHLQVKITRARFEQLSQPLVKRLRGPFEQALKDAKLSPADIDEVILVG
                     GATRMPMVQQLVSELAGGKEPNKSVNPDEVVAVGAAIQAGVLGGEVKDVLLLDVTPLS
                     LGVETLGGVMTTLIPRNTTIPYKKSEVFSTADDNQTAVDIHVLQGERPMAADNMTLGR
                     FRLEGIPPAPRGMPQVEVTFDIDANGILHATARDKATGREQKIAITASTNLSKSDVER
                     MVQEAQRNASEDRRRAELVQARNEADTVIYQAEKSLRDLGDKVAAADRQRVESQMAAL
                     KSAMNGDDAAHIRRLIGELQQATLAIGQSMYQTPDSGGSTGPGSGRPEGEDVIEGEFR
                     QV"
     gene            3232..3924
                     /locus_tag="AC162G_04780"
     CDS             3232..3924
                     /locus_tag="AC162G_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HIR5075162.1"
                     /translation="MLFFDPMYFVFILPALLLAFYAQMKVRSAYGKWLKVPNTRSLSG
                     ADAARYLIGANQIGDVRIEGVPGELSDHYDPRTKTLRLSQGVANSRSVASLAIVAHEV
                     GHAVQDATDYGPLKLRGSIVPAIQVSAWIAPLLFIVGLLINSTGLAWLGVILFGGTVV
                     FALITLPVELNASARAMAMLRDNGLVASDQDEKGAGSVLNAAALTYVAALAQTLMTLL
                     YYVTILTGSRRD"
     gene            4232..4681
                     /locus_tag="AC162G_04785"
     CDS             4232..4681
                     /locus_tag="AC162G_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR5075163.1"
                     /translation="MSSGIIRWEPFREMISLRDAMNRLFEDSFVRPAAWPAAIEGMPL
                     AVDVMETKDDVVVKTSVPGIQPDDLDITLSGETLTIKGEFKSEQKTEDATYLRQERRY
                     GSFERSLTLPTQVVGEKAKAEFENGVLTLTLPKAEEVKPKSIKVHTK"
     gene            complement(4885..5079)
                     /locus_tag="AC162G_04790"
                     /pseudo
     CDS             complement(4885..5079)
                     /locus_tag="AC162G_04790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
     gene            5241..6215
                     /locus_tag="AC162G_04795"
     CDS             5241..6215
                     /locus_tag="AC162G_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014476.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR5075164.1"
                     /translation="MNSQELLYLSRADVESVALDMRTIINLLEAAFKEKGEGRVEMPP
                     KPGIHTQPDAFIHAMPAYIPSLRSAGLKWVSGYPENMRRNLPYITGLLILNDVETGIP
                     YAVMDCTWITAYRTGAASALSARYLARPDSEVAGILACGVQGRTNLQALATLFPIKRV
                     YAYDIVPAVQQHFVDEMKARLGLDVIGVQEPREAVVESDLVVTSGPILKHPTPTIEKG
                     WLRPGAFASAVDFDSYWTPEALAQFDKLSTDDYAQFQYYRSAGYFQSTPDPYADLGEI
                     VAGLKPGRERSDERTMPMNLGLALDAMAVAPEIDRRAKERGIGTRLPL"
     gene            complement(6289..7815)
                     /gene="amaB"
                     /locus_tag="AC162G_04800"
                     /gene_synonym="amaA"
     CDS             complement(6289..7815)
                     /gene="amaB"
                     /locus_tag="AC162G_04800"
                     /gene_synonym="amaA"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-piperidine-6-carboxylate dehydrogenase"
                     /protein_id="HIR5075165.1"
                     /translation="MTKALLSKLNIQDVNPGACTGPDAWIHDPRGQPLVSYNPTTGEA
                     IASVMQATPATYDEVARRAEAAFKTWCETPAPKRGQLVRDLGNALRELKEPLGDLVSL
                     EMGKIRAEGRGEVQEMIDICDFAVGLSRQLYGLTMHSERPAHRMYEQWHPLGVVGVIT
                     AFNFPVAVWSWNATIAAVCGDPVLWKPSQVVPLCAIAVQHIANRVMADHGVMGIFNLV
                     IGKGSEVGDLLLNDPRLPLISFTGSTQVGRRVAENVARRFGRTILELGGNNAIIVAED
                     ADLDLATRAILFGAAGTAGQRCTSTRRIIMHKDIAANLSDRLVRAYLQVRIGDPLEDG
                     TLMGPLVNGTAVEDMLDALEQAKAQGGKILVGGRMRPDLGPNFVEPTIVKMPAQTDIV
                     RCETFAPILYLLEYQTLDEAIRIHNDVPQGLSSAIFTDSMRQAEAFLSVHGSDCGIAN
                     VNIGTSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRQTNTINWSKDLPLAQGIKFGD
                     "
     gene            complement(7881..9788)
                     /locus_tag="AC162G_04805"
     CDS             complement(7881..9788)
                     /locus_tag="AC162G_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075166.1"
                     /translation="MDSMLPGQSVEEVERRSIPRLRIALGLIVLSAAAVVCVLCEQLQ
                     GSAERGDDTARPTAARDALPKPAVRAGETIVGSGPVALAFDARRARIWVANSNDNAVQ
                     SIDLTTGSVSRRIAVGANPRALAFDALRARLWVANRDDSTVQAIRVTTAQTEPPIPVR
                     LHPFALAFDGARLWVANEGDSSVQSIDPVTGAIGQTISVGAGPRALVFDGARLWVANA
                     FGNSVQAIQVEANEISPPISVGEFPVALAFDTLRARLWVANFFDNTVQWVDPVTGEAG
                     QPVPVAERPQALAFDGQRLWVTNYGNRTVQSIDPINGNVSRPIPVGALPAALAFDGTW
                     LWVANSFDNSVQPIDPTSGRVSGPLVVGANPRALAFDGSRLWVANADSHTVQAVSVTT
                     GQVSPRIRVGRSPDALLFDGTRLWVANAFDNAVQPIDPETDVAGRPIPVGRTPQALAF
                     DGTQLWVANRDDNTIQAVGAATGDVLSPIPVGAFPTALIFDGTQLWVANWGDDTVQSI
                     DPVTGKVNPPRPVGAFPTALAFDGQRLWVANRGDDSVQSVDPATGAVSQSIRVGAAPS
                     AIVFDGVQLWVANWGEDTVQSIAPATGDVTLSITVGAFPWALAFDPIHARLWVANSVD
                     NTVQSIDVSTQ"
     gene            10133..>10461
                     /locus_tag="AC162G_04810"
     CDS             10133..>10461
                     /locus_tag="AC162G_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR5075167.1"
                     /translation="MKHIKLISSLLVIAAVVLAACGPGAPAFTPPTDELGLVTIPPGE
                     PIHIAYWGVLSGADASLGEDSKRGVELQIDAHGGKLLDRDIRLTTEDAGCIPEGGATA
                     GQKLAADT"
BASE COUNT         2040 a         3327 c         3248 g         1846 t
ORIGIN      
        1 ggctcggaga acggttccgt cgtgtaccac ccccggccat cttcccagat caccggcggg
       61 ttgaggcatt cttcgatcgt tgtccatatc gagaacgtgg gtgcgaacgc gtaaccgcgt
      121 acttccagat tggatccgtc gcgcacgccg atctcgtcga tctcgtcgaa caggtgcttc
      181 tcggcgtagc gggcgaacac gtcagacagg ccaggctcga cgcccatgcc gaccagggcg
      241 agcaggccct tcttttccca tttctccgac tgctcgaact gataatcgcc gagcttcaca
      301 ccggcctgcc gatatggctg atccggatgc ggcctggaca gggtcatcgc catgtccagg
      361 tacgtgcagc ctgtctcgta ggccgcctcg aagatcggct cgtcgaatac cggatcgacg
      421 gcgttcaaga tcagatcgac gccgtgtttc ctggcgagcg ccacgatccg ctccttgtcg
      481 ccggcgtcga tccattcggg cggatacttg ctcgcggtgg tctcgccgag cttcgccctt
      541 acctcctcga cacgcgcgac gtcgtagtcc gccaggacca tcttttcaag ccagggacgg
      601 cattgggccg tgacggcgat cgcttccccg acgccgccca cgccaatcag taggactttc
      661 atggtgggtc tcctctaatc ggtgaatggt ggtcagtgta cgatagggtg tattagaggt
      721 tcgattgtgt tggggaattg gaagcgagaa gttggaggct ggagttccca cctgccaatt
      781 gtctatcacc acccttctac aggctcaccc gctcaagcac acgtcaattt tatgcgagtt
      841 tctacaaacg cacaagcacg caggcgctcg agcggcagac gtcagcgtga aagccttaga
      901 ttctatccga aaagcggagg cggctccttg cgaagcaccg tgtaggtgtc ctaagtcgtg
      961 tcaagcccca attcgcgggc ttgaatcgta atcccacgct aatgtcaatc taatgcgcgt
     1021 ctaacagtga tctaatgcct gattattaga atacaggtga ctacttagcc gtcattgcaa
     1081 ggagcgccgt ttgcgacgaa gcaatctcag ccgccctgag tgcgcgggtc tgtccgtgcc
     1141 ggagattgct tcgcaaaaag cgctcgcaat gacgcgggct gagcagttac gaatgcagaa
     1201 acactggagg atggagaaac caactttctg aaggagaacc tcgagatggg caagattata
     1261 ggtatcgatc tgggaacgac gaactcggtg gcggccgtcc tggaaggcgg tgacccggtc
     1321 gtcatcacga cggcggaggg cagccgcctg ctgccgtcgg tcgtggcttt cgccaagaac
     1381 ggcgagcgtc tggtcgggca gacggccaaa cgccagtccg tgatcaatcc cgagaacacg
     1441 atctttagca tcaagcgctt catgggcaag cgcgagcagg acgtgcgcca cgagaaagtg
     1501 ccgtacaggg tcgtcgccgg cccgagcggc gacgcgcgcg tgcatgtgcc gcaggccggc
     1561 aaggatttca cgccgcagga aatctcggcg atgatcttgc agaagctgaa gaaggacgcc
     1621 gaggcttatc tgggcgagcc ggtgtctcag gccgtgatca ccgtgccggc ctacttcaac
     1681 gattcgcagc gccaggcgac gaaagacgcc ggcaagatcg ccggcctgga agtcttgcgc
     1741 atcatcaacg agccgaccgc agccgcgttg gcttacggcc tcgacaagaa gaaggatgag
     1801 acgatcctcg tcttcgacct gggcggcggc acgttcgacg tgtccttgct cgaagtcggt
     1861 gagggcgtcg tcgaggtgaa ggcaacgagc ggcgacacgc acctgggcgg cgacgactgg
     1921 gatcaggttc tcgtcgacta cgtcgccgac gagttcaagc gcgagcaagg catcgatctg
     1981 cgccaggatc gccaggcgct tcagcgactg cgcgaggcgg cggagaaatc aaagatcgag
     2041 ctgtccagtg gcatggagac ggagatcaat ctgccgttca tcaccgccga cgcctcgggt
     2101 ccgaagcacc tgcaggtgaa aatcacccgc gccaggttcg agcagctcag ccagcccctc
     2161 gtcaagcgac tgcgcgggcc gttcgagcag gcgctgaagg atgcgaagct gtcgccggcg
     2221 gacatcgacg aagtcatcct ggtcggcggc gcgacgcgca tgccgatggt gcagcagttg
     2281 gtgagcgagc tggccggcgg caaggagccg aacaagagcg tcaacccgga tgaagtggtg
     2341 gcagtcggcg cggcgatcca ggccggcgtg ctcggcggcg aggtcaagga cgtcctgctg
     2401 ctcgacgtga ccccgctgtc gctgggcgtc gagacgctgg gcggcgtgat gaccacgctc
     2461 atcccgcgca acacgacgat cccgtacaag aagagcgagg tcttcagcac tgccgacgac
     2521 aaccagacgg ccgtggacat tcacgtcctg cagggtgagc gcccgatggc cgccgacaac
     2581 atgacactcg gtcgcttccg cctcgaaggc attccgcctg cgccgcgcgg catgccgcag
     2641 gtcgaggtga cgttcgacat cgacgccaac ggcatcctgc acgcgactgc cagagacaag
     2701 gcgacgggcc gcgagcagaa aatcgccatc acggcttcga ccaacctgtc caagagcgac
     2761 gtcgagcgca tggtgcagga ggcgcagcgc aacgcgtctg aggatcgccg ccgcgcagaa
     2821 ctggtacagg cgcgcaacga ggccgacacg gtcatctacc aggccgagaa gagcctgcgc
     2881 gatctgggcg acaaggtagc cgccgcggac cgccagcgcg tcgagtcgca gatggccgcg
     2941 ctcaagtcgg cgatgaacgg cgacgacgcg gcgcacatcc gccgcctgat cggcgagctt
     3001 cagcaggcca cgctggcgat cggccagagc atgtaccaga cgccggattc cggcggctcg
     3061 accgggccag gcagcggccg gccggaaggc gaagacgtga tcgagggaga attccgtcag
     3121 gtttaaggca gccacacccg gcccgttccg ggtataatta gagaagctct ggagaaacct
     3181 ggtttctcag agaaaccagg tttcttcaat gcgcagccct ggaggtagca catgttgttc
     3241 ttcgatccca tgtatttcgt gttcatcttg ccggcgctgc tgctggcctt ctacgcacag
     3301 atgaaagtgc gcagtgccta cggcaagtgg ctgaaggtgc ccaacacgcg cagtctctct
     3361 ggcgcagacg ccgccagata tctgattggc gcaaaccaga tcggcgacgt caggatcgag
     3421 ggcgtccccg gcgagctgtc cgaccactac gatccacgca caaagacatt gcgcctgtcg
     3481 caaggcgtcg cgaattcacg ctcggtcgct tcgctggcga tcgtcgcgca cgaggtcggc
     3541 cacgccgtgc aggatgcgac cgattacggg ccgctgaagc tgcgaggctc gatcgtgccc
     3601 gccattcagg tcagcgcctg gatcgctccg ctcctgttca tcgtcggcct gctgatcaac
     3661 agcacggggc tggcctggct gggcgtgatc ctgttcggcg gcaccgtcgt gttcgcgctg
     3721 atcacgctgc cggtggagct gaacgcgtcc gcccgcgcta tggcgatgct gcgcgacaac
     3781 ggtctcgtcg ccagcgatca ggacgagaaa ggcgcgggca gcgtgctgaa cgccgccgcc
     3841 ttgacctacg tcgcagcgct ggcccagacg ctcatgacgc tgctgtatta cgtcacgatc
     3901 ctgaccggca gccggcgcga ctgatcttgt cacgcggact tctcccggat cacacggatt
     3961 ggcaagcatt ccggcacggt caaggcgcaa cgggatagtt gccaagtcca gagcccaaaa
     4021 acgtcccccg ggaaatcggt gtgtcagagt ggagaaacct ggtttctcag ggaaaccagg
     4081 tttctttaac acgggccagt aatactcctg ggtcgttcct tttttacgac agaggattgc
     4141 aaatgccgtt ccaacgcaga attaacgttc ctctaatgac agcgtgctat tatgatcgtg
     4201 ccattgaaaa ccgacacgca ggaggatagc catgtccagc gggataattc gatgggagcc
     4261 gttccgcgag atgatcagcc ttcgcgacgc gatgaaccgc ctgttcgagg attcgttcgt
     4321 ccgaccggcg gcgtggccgg cggccatcga gggcatgccg ctcgccgtgg acgtcatgga
     4381 gacgaaggac gacgtggtcg tcaagacgtc ggtaccgggc atccagcccg acgatctcga
     4441 catcacgctc agcggcgaga cgctgaccat caagggcgag tttaagtccg agcagaagac
     4501 ggaggacgcc acgtacctcc gccaggagcg gcgctacggc tcgttcgagc gcagcctgac
     4561 gctgcccacg caggtagtgg gtgagaaggc gaaggccgag ttcgagaacg gcgtgctcac
     4621 cttgacgctg ccgaaggccg aggaagtgaa gccgaagagc atcaaggtgc acaccaagta
     4681 agagcttatc tgaaaagtgg agtgcagact cacctgcccc cgtagcgaag cggagtgggg
     4741 tgagtctgcg cggccaaagc gggctaaagc cccgcactcc ggaggttttc ggataggcac
     4801 tgagcggaag tcccgcccaa tctaaaaagc cactcgcgcg tatggcgcga gtggcttttc
     4861 tatctactgc tcccgccggg tgtgtcaccc ggcggcccgg gtcgcaatca gttgcctgat
     4921 ttcgccgggt tccgcgtgcc ggcgcgttgc cagcttgctg tacacgagat cactgttgac
     4981 ggtaacctcg aatcgcccgc cgctcgacgg gatgagcgtg accgattcga tctgcgactc
     5041 aaatgcgccc agcagttccg ccgtgagact cacggctcgg tcggtgtacc ttcagactgc
     5101 acaatattcg atcgtgactt tgaccatctt tcgcctcctt gatatgaata tgcactttcg
     5161 tgtcgtgtgt cacttgtctc ttcacctgga aacgggtatg ctgtgcaaga gccacaccca
     5221 gacatccagg agaaatcggc atgaactccc aagagttgct ctatctgtct cgcgccgacg
     5281 tggaaagcgt ggcgctcgac atgcggacga tcatcaacct gctcgaagcc gctttcaaag
     5341 agaagggcga gggtcgggtg gaaatgccgc ccaagcccgg catccacacg cagccggacg
     5401 cgttcattca cgccatgccc gcctatattc catcgctccg atcagccggc ctcaagtggg
     5461 tgagcggcta tccggagaac atgaggcgca atctgcccta catcacgggc ctcctgatcc
     5521 tcaacgacgt ggagacggga attccgtacg ccgtcatgga ctgcacgtgg atcacggcgt
     5581 atcgcaccgg ggccgcgtcg gcgctctcgg ctagatacct ggctcgcccc gacagcgaag
     5641 tagccggcat tctggcttgc ggcgtccagg ggcgaaccaa cctgcaggcg ctggcgacgc
     5701 tgttccccat caagcgcgtt tacgcctacg acatcgtgcc cgccgtgcag cagcacttcg
     5761 tcgacgagat gaaagcgcgg ctgggcctgg acgtcatcgg cgtgcaagag cctcgggaag
     5821 ccgtcgtcga gagcgatctg gtcgtcacct ccgggccgat cttgaaacac cccacaccga
     5881 ctatcgagaa aggctggctg cggcccggcg cattcgccag tgccgtcgac ttcgacagct
     5941 actggactcc tgaagcgctg gcgcagttcg acaaactcag caccgacgat tacgcgcagt
     6001 tccagtatta caggagcgcc ggctacttcc agagcacacc cgatccgtac gccgacctcg
     6061 gcgagatcgt agccggactc aaacccgggc gcgagcggag cgacgagcgc acgatgccga
     6121 tgaacctggg tctggcactc gacgccatgg ccgtcgcgcc ggagattgac cgccgcgcga
     6181 aagagcgcgg catcggaacc cggctgccct tgtagaactt cacgtgcgac gcactttcat
     6241 agacccccgt agcgaagcgg aggggcagaa gtgcgtcgca cgttaaattc aatcgccgaa
     6301 cttgatccct tgcgccagcg gcaggtcctt cgaccagttg atggtgttcg tctggcggcg
     6361 catataggct ttccacgcgt cggaaccgga ctcgcgcccg ccgccggtct ctttctcgcc
     6421 gccgaacgcg ccgccgatct ccgcgcccga cgtgccgatg ttcacgttgg cgatgccgca
     6481 gtccgatcca tgcaccgaca ggaacgcctc ggcctggcgc atcgagtcgg tgaagatcgc
     6541 gctcgacagg ccctgtggca cgtcgttgtg aatccgaatc gcctcgtcaa gcgtttgata
     6601 ttcgagcaga tacagaatcg gcgcgaaggt ctcgcacctg acgatatccg tctgcgccgg
     6661 catcttgaca atcgtcggct cgacgaagtt cgggccaagg tcggggcgca tcctgccgcc
     6721 gaccaggatt ttgccgccct gcgccttcgc ctgctcgagc gcgtcgagca tgtcctcgac
     6781 tgccgtcccg ttcaccagag gccccattag cgtgccgtcc tccagtggat cgccgatgcg
     6841 cacctggaga taagcgcgca caaggcgatc gctcaggttg gcggcgatgt ccttgtgcat
     6901 gatgatccgc cgcgtggagg tgcagcgctg gccggcggtg cccgccgcgc cgaacaggat
     6961 cgcccgcgtc gccagatcga gatcggcgtc ctcggcgacg atgatggcgt tgttgccgcc
     7021 cagctcgaga atcgtgcggc cgaaccggcg cgccacgttc tccgccacgc gccgcccgac
     7081 ctgcgtcgag ccggtgaacg agatgagcgg caggcgcgga tcgttcaaga gcaagtcgcc
     7141 gacctcggag cctttgccga tgacgaggtt gaagatgccc atcacgccgt gatcggccat
     7201 cacgcgattg gcgatgtgct gcacggcgat cgcgcacagc ggcacgacct gagacggttt
     7261 ccacagcacc gggtcgccgc acacggctgc gatcgtcgcg ttccacgacc acacggccac
     7321 cggaaagttg aaagccgtga tcacgcccac cacgccgagc gggtgccact gctcgtacat
     7381 gcggtgcgcc gggcgctcgg agtgcatggt caggccgtac aactggcgcg ataacccgac
     7441 ggcgaaatcg cagatgtcga tcatctcctg aacttcgccg cgcccctcgg cgcgaatctt
     7501 gcccatctcc aggctgacca ggtcgccgag cggctccttc agctcgcgca atgcattccc
     7561 caggtcgcgc acgagctggc cgcgcttcgg cgccggcgtt tcgcaccacg tcttgaaagc
     7621 ggcctccgcc cggcgcgcga cctcgtcgta ggtcgcgggc gtcgcttgca tgacgctggc
     7681 gatggcttcg cccgtcgtcg ggttgtagga gacgagcggc tggcccctcg gatcgtgaat
     7741 ccaggcgtcc ggcccggtgc acgcgccggg gttcacgtcc tggatgttta atttggatag
     7801 caaagctttc gtcatgagaa ttcctcctcg caggggcaac cgtaggggca accgcaaggg
     7861 ttgcccctac gatggcgctc ttactgggtg gacacgtcga tcgattgtac tgtattgtca
     7921 acactgttcg ccacccacag ccgcgcgtga atcggatcga aggcaagcgc ccagggaaat
     7981 gcaccgacag ttatcgacag ggttacatca cctgtcgccg gcgcgatcga ctgcacggtg
     8041 tcctcgcccc aattcgcgac ccacaactgc acaccgtcga agacgatggc agacggcgct
     8101 gcgccgaccc gtatcgactg gctcacagcg cccgtcgcag ggtcgaccga ctgcacgctg
     8161 tcgtcgcccc gattcgccac ccacagccgt tgaccgtcga aggccagagc ggtcggaaac
     8221 gcgccgacgg gtctaggtgg gttcacttta cccgtcacag gatcgatcga ctgtaccgtg
     8281 tcgtcacccc agttcgctac ccacaattgt gtgccgtcga agatcagggc agtcggaaac
     8341 gcgccgacgg gaatcggaga gaggacgtca cccgtcgccg cgccgacggc ctgtatcgtg
     8401 ttgtcatcgc gattggcgac ccatagctgt gtgccgtcga aggccagagc ttgcggcgtc
     8461 ctgccgacgg gaatgggccg acccgcgacg tccgtttcgg ggtcgatcgg ctgcaccgcg
     8521 ttgtcaaaag catttgccac ccacagccgc gtcccatcga ataacagcgc atccggtgac
     8581 ctgccgacac gaatccgcgg actcacctgt ccggtcgtga cggagacggc ctgcacggtg
     8641 tggctatccg cattcgccac ccacagtcga gaaccgtcaa aagccagtgc gcgcggatta
     8701 gcgccgacga cgagcggccc gctcacgcgg cccgatgtcg gatcgatcgg ctgtaccgag
     8761 ttatcgaacg aattcgcgac ccacagccac gtgccgtcga acgccagtgc ggccggcagc
     8821 gcgccgactg ggatcggccg gctcacattt ccattgatcg ggtcgatcga ctgcacggtg
     8881 cggttgccgt aatttgtgac ccacagccgc tgcccatcaa aggccagggc ttgcggacgt
     8941 tcggcgacgg gaaccggttg gcctgcctcg cccgtcacgg gatcgaccca ctgcacggtg
     9001 ttgtcgaaga agttggccac ccacaggcgc gcgcgaagcg tgtcgaaggc aagggcaacc
     9061 ggaaattcgc ccactgagat cggggggctt atctcattcg cctccacctg gatcgcttgc
     9121 acggagttgc cgaaggcatt cgcgacccac aagcgcgccc cgtcgaagac cagtgcgcgc
     9181 ggaccggcgc cgactgaaat cgtctggcca atcgcgccgg tcaccgggtc gatcgactgc
     9241 acactgctgt ctccttcgtt cgcgacccac agccgtgcac cgtcgaaggc tagagcgaac
     9301 ggatgcaggc ggacgggaat gggcggctcc gtttgagccg tcgtgacgcg aatggcctgc
     9361 acggtgctgt catcacgatt cgcgacccat agccgcgcgc gaagcgcgtc aaaggccagc
     9421 gcgcgtggat tcgcgccaac agctatacgc cgactcacgc tgcccgtagt cagatcgatc
     9481 gactgcaccg cgttgtcgtt gctgtttgcc acccatatcc gcgcgcgccg cgcgtcgaag
     9541 gcgagagcca ccggccctga gccgacgatt gtctctccgg cgcgcacggc aggctttggc
     9601 agcgcatctc gtgcagccgt cgggcgcgct gtgtcgtccc cccgctcggc cgatccttgg
     9661 agctgctcgc aaagcacgca taccacggca gccgctgaca atacaatcaa cccgagcgcg
     9721 atacgcaacc tgggtatgga tcgcctctcg acttcttcca cactctggcc cggcagcata
     9781 ctgtccatac gacggcactc agaaaggggc tggggatatt gtagaccaat gctgccagcc
     9841 gtcaacgctt tcgcttgaca aaggctagta gccgtaatac aatgacgcca ctattcagcc
     9901 ctggggcagt agggcagcct ctcagaacag ccacgccgaa aaatcttatc cgtgcttaac
     9961 gcacgaagac aactgggaag gaggtgaggg cttcctcaag ttgcctatac cgtttgagca
    10021 gccagcgaga cgtcgggctg gaaccccgcg cgcactgcgt actcgtctgc tcccacaacg
    10081 tgacaaagcg actcgccgta gtcattcttc ttcaaaggag gagaggatta ccatgaagca
    10141 catcaagttg atcagttcgc tgctcgttat cgccgccgtc gtgctggcag cctgtggtcc
    10201 gggtgcgccc gcattcacac cgccgaccga tgagctcgga ctcgtgacga ttccgccggg
    10261 cgagccgatc cacatcgcct actggggcgt gctctccggc gcagacgcct cgctgggcga
    10321 ggattccaaa cgcggtgttg agctccaaat cgatgcccac ggcgggaaat tgctcgaccg
    10381 cgacatccgc ctgactaccg aggacgctgg gtgcatacct gagggcggcg cgacagcagg
    10441 gcaaaagctg gccgccgaca c
//
LOCUS       DBNHXB010000125         3098 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2206186, whole genome shotgun sequence.
ACCESSION   DBNHXB010000125 DBNHXB010000000
VERSION     DBNHXB010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2206186"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="AC162G_04815"
     CDS             complement(<1..909)
                     /locus_tag="AC162G_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014750.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5075168.1"
                     /translation="MSYGYAGAILRINLTDRRVTREPLERKLCEDYVGGRGLTSYLLY
                     REMDPAVDPLSSENKLMFGAGPLTGTGAVAASRYVVVCKSPLTGTIACSNSGGFFGPE
                     MKFAGYDVIVFEGRAEKPAYVWINDDQVEIRPADHLWGKTPEETDDAVRAATSPLAKV
                     ACIGPAGEKLSLLAGVVNDRQRLAARSGVGAVMGIKNLKAVAVRGTGSIGLADPEAFW
                     FAALDVLKKVVNDPVSFGSFRAYGTANLVNLINEMGAYPTRNFQEEVFEGAAATSGEA
                     IAEHILLKNRACFACPIACTRVSEVKD"
     gene            complement(999..2747)
                     /locus_tag="AC162G_04820"
     CDS             complement(999..2747)
                     /locus_tag="AC162G_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5075169.1"
                     /translation="MATEYGADVVIVGGGIVGCAAAYYLARRGASVILLEKNGIGSGA
                     SGRSGGGVRQSARASAELPLANEAVALYPTLSDELGVDIEYVQRGNLRLVEMVDHIRP
                     MQVDIARQQAMGLDVHWIEQADVCEMVPSLRRESVFGASFCPTDGHANPLKLTAGYAA
                     AASRAGAKILTGCEVRAVRQTDSGEAVVETTRGDVRARTVIITTGAGTRSLCLNLGVD
                     LPLANMRYESLVTEALPPLFPYMFGVASADLFFRQTRHGSIHFGGGLIQQAEDEATTT
                     KNVQLAAEHIVRLIPDLGQVSLVRTWGGLDPSTPDGIPIIDRLNENILVAAGFCGHGF
                     AIGPVVGRHLAEWVVSGKKPAALEPFRRNRFEGLLQTKWTPSGSFEAVLAAEPTQMVS
                     GDKRIAATAVGSEIAVQPDGKRFLLINPDLCTGCRMCEMACSIHHTFAARSTGLRIQV
                     AYKSDHEFSPVPCIHCEEAYCMDVCPVNALVRDGDTAVIRVVDEDCIACMLCIDACPY
                     GGISYSEEKGVVIKCDLCGGDPSAGSGQAPSTGPACAAYCAPGAIRFRVIDEPTWVQM
                     KANAEEYAKLREQGLK"
     gene            complement(2912..>3098)
                     /locus_tag="AC162G_04825"
     CDS             complement(2912..>3098)
                     /locus_tag="AC162G_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075170.1"
                     /translation="SPVAAQIVATEAGVSVESVGAFTARIPIKPVPLGLVASAGVEQP
                     APTAMEDHVGYGKAIIR"
BASE COUNT          563 a         1031 c          947 g          557 t
ORIGIN      
        1 gtccttcacc tcgctcacgc gggtgcacgc aatcgggcag gcgaagcacg cccggttctt
       61 caacaggata tgctcggcga tggcttcgcc gctcgtcgcc gctgcgccct cgaagacctc
      121 ttcctgaaag ttgcgcgtcg ggtacgcgcc catctcgttg atcagattga ccaggttggc
      181 tgtcccgtaa gcgcggaagc tgccgaacga caccggatcg ttgacgactt tcttcagcac
      241 gtcgagcgcg gcgaaccaga atgcctccgg gtcggccagg ccgatgctgc ccgtgccgcg
      301 cacggcgacg gctttgagat tcttgattcc catgaccgcg cccacgccgg atcgcgccgc
      361 cagccgctgg cggtcgttca ccaccccggc cagcagcgac agcttttcgc ccgccgggcc
      421 gatgcaggcg accttcgcca gcgggctggt ggcagcccgg acggcgtcgt cggtctcctc
      481 gggcgtcttc ccccacaggt gatccgccgg gcggatttct acctggtcgt cgttgatcca
      541 cacgtatgcc ggcttctcgg ccctgccttc gaagacgatc acgtcgtagc ctgcgaactt
      601 catctccggg ccgaagaatc cgccggagtt ggagcatgcg atcgtgccgg tgagcggcga
      661 cttgcacacg acgacgtagc gcgatgcggc aaccgcgccg gtgccggtga gcggcccggc
      721 gccgaacatc agcttgttct ccgaactgag ggggtcgacg gcaggatcca tctcccgata
      781 cagcaggtac gacgtgaggc cgcgcccgcc gacgtagtct tcgcacagct tgcgctcgag
      841 cggctcgcgc gtcacacggc ggtcggtaag gttgatgcgc agaatggcgc ctgcgtaacc
      901 gtaggacatt ggaaatccaa agatcaaact ccaaatctca aactccaaaa ttcaaacgta
      961 gtctgtaatt cggagtttgg attttgaagt ttgtagtttt acttcaaccc ttgttccctc
     1021 aacttcgcat actcttctgc gttcgccttc atctgcaccc acgtcggctc atcgatcacg
     1081 cggaagcgga tcgcgccggg ggcacaatag gcggcgcacg ccggacctgt cgaaggagcc
     1141 tgccctgagc ctgccgaagg atcgcctccg cacaggtcgc acttgatcac cacgcccttc
     1201 tcctcggaat aggagatgcc cccgtacggg caggcgtcga tgcacaacat acacgcgatg
     1261 caatcctcgt ccaccacccg gatgacggcg gtgtcgccgt cgcgcacgag cgcattcacg
     1321 gggcacacgt ccatgcagta cgcctcttcg cagtggatgc aggggacggg tgaaaactcg
     1381 tgatcgctct tgtacgcgac ttgaatgcgc agccctgtgg atcgagcggc gaaagtgtgg
     1441 tgaatggagc aggccatctc gcacatgcgg cagccggtgc agagatcggg attgatcagg
     1501 agaaatctct tgccgtctgg ctggactgcg atctccgatc caaccgccgt cgccgcaatg
     1561 cgcttatcgc cgctgaccat ttgcgtgggc tcggcagcca ggacagcctc gaacgaaccg
     1621 gacggagtcc acttcgtctg cagaagaccc tcgaagcgat tgcgccggaa cggttcgagc
     1681 gcagccggtt tcttcccgga tacgacccac tcggcgagat ggcgaccgac caccgggccg
     1741 atggcgaagc cgtgcccgca gaaaccggcg gccaccagga tgttttcgtt gagccgatcg
     1801 atgatcggaa tgccgtcggg cgtggacgga tcgaggccgc cccacgtccg taccagactc
     1861 acctggccca gatcgggaat gagacgcacg atgtgctcgg cagccagttg cacattcttc
     1921 gtcgtcgtcg cttcgtcttc agcctgttga atcaatccac cgccgaagtg aatgctgccg
     1981 tgccgcgtct gccggaagaa cagatcggcg ctcgccacgc cgaacatgta cgggaagagc
     2041 ggcggcagag cctcggtgac gagtgactcg tagcgcatat tcgccagagg tagatcgacg
     2101 ccgaggttga ggcacagcga gcgcgtccct gcgcccgtgg taataatgac cgtgcgcgcc
     2161 cgcacgtcgc cgcgtgtcgt ttcgacgacg gcctcgccgg aatcggtctg ccggacggcg
     2221 cgcacttcac aaccggtaag gatcttcgcg ccggcgcggc tcgccgcagc ggcgtatcct
     2281 gccgtgagct tcaagggatt cgcgtggccg tcggtcgggc agaaactcgc gccgaacacg
     2341 ctctcacggc gcagcgacgg aaccatctca catacgtcgg cctgctcgat ccagtgcacg
     2401 tcgaggccca tcgcctgctg ccgggcaatg tcgacctgca tggggcggat gtgatccacc
     2461 atctcgacga gccgcaggtt gccgcgctgg acgtattcga tatctacgcc cagctcgtcc
     2521 gaaagtgtag ggtagagtgc gacggcttca ttcgcgagag gcaattcggc ggaggcgcgc
     2581 gccgactgcc gcacgccgcc ccccgatcgc cccgaagcgc cgctgccgat gccgttcttt
     2641 tcgagcagga tcacgctcgc gccgcgccgc gccaaataat acgccgccgc gcacccgacg
     2701 attcccccgc ctacgatgac tacgtctgca ccgtattcag tcgccatgcg agaactcacg
     2761 cggtcaatca cctaaagatg ctatgctcgt cgcagacgtg tcatgccgag gagcgcgtag
     2821 cgcgacgagg catctctgcc ctggcgcgcc gcgctcgccg gcctgaggag agattcctca
     2881 ctgcgttcgg aatgacacac tgcctggtgc atcaccgaat gatcgctttt ccatacccca
     2941 cgtgatcttc catcgccgtc ggagcaggct gctcgacgcc tgcactggcg acgaggccga
     3001 gcggcacagg cttgatcggg atccgcgccg tgaacgcgcc gacggactcc acactcacac
     3061 ccgcttcggt ggcgacaatc tgcgcggcga cagggctg
//
LOCUS       DBNHXB010000126         4909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3044593, whole genome shotgun sequence.
ACCESSION   DBNHXB010000126 DBNHXB010000000
VERSION     DBNHXB010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3044593"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="AC162G_04830"
     CDS             complement(<1..320)
                     /locus_tag="AC162G_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR5075171.1"
                     /translation="MKRIDQRTEHRLQSESNIWVATVRPDGRPHLTPVWFVWHDGKVY
                     ICIDPNSVKARNMTRNDRVALALEDGSSPIIFEGVGRAVGASDWPQGAIDGFKRKYDW
                     NIVT"
     gene            complement(373..2973)
                     /gene="clpB"
                     /locus_tag="AC162G_04835"
     CDS             complement(373..2973)
                     /gene="clpB"
                     /locus_tag="AC162G_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR5075172.1"
                     /translation="MNLNKFTEKAQEAVLGSQRLAEEFGHQAIEPEHLLLSLLRQGEG
                     VAPQLLQKLGQRPEQIAAIVERDLQGRPRVSGSNTQVGLSRAANDVLSAAENEARSMR
                     DDFVSTEHILIALARSRTALGSMLNARGVSAQAILQALVSVRGSTRVTSQHPESTYQA
                     LEKYGRDLTQLARQGKLDPVIGRDDEIRRVIQILSRRTKNNPVLVGEAGVGKTAIAEG
                     LAQRIVRGDVPEGLKDKRIVALDMGALVAGAKYRGEFEERLKAVLKEVADSTRVILFI
                     DELHTVVGAGAAEGAMDASNMLKPMLARGELHTIGATTLDEYRKHVEKDAALERRFQP
                     VYVDEPGVEETISILRGLKERYEVHHGVRIQDSAVIAAATLSNRYITDRQLPDKAIDL
                     IDEAASRLRMEIDSKPAALDEVDRKIMQLEIEREALKKERDKASRERLEKIEKELGDL
                     KEESSALRAHWELEKNAITSLRAIKEKIEATRHEIEVAERSADLETAARLRFGTLRDL
                     EKQLAEGEARIKELQKGQRMLKEEVEAEDVAEVVAKWTGIPVSRLLEGEVQKLLHMED
                     RLRRRVVGQDDAIRVVSNAVRRARAGLQDRSRPIGSFIFLGPTGVGKTELARSLAAFL
                     FDDESAMVRIDMSEYQEKHTVSRLIGAPPGYVGYDEGGQLTEAIRRRPYSVVLFDEIE
                     KAHPEVFNALLQLLDDGRLTDGHGRTVDFKNTLVIMTSNVGSQWIKELGGGDPAEMRR
                     RVMAAMEQSFKPEFLNRVDEIVIFNNLTRDDLARIVEIQLVSLRRLLADRKIDLQLSD
                     RAKHWLADKGFDPVYGARPLKRTIQRELQDPLALKILSGVYGEGDVVSVDANEDGLTF
                     VSEERMAEAA"
     gene            complement(3179..4105)
                     /locus_tag="AC162G_04840"
     CDS             complement(3179..4105)
                     /locus_tag="AC162G_04840"
                     /inference="COORDINATES: protein motif:HMM:NF025616.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HIR5075173.1"
                     /translation="MNTLTKLALFCTLTAVAVIAAACAAPAATPAPRFNAADGAGLPE
                     AVIVTAAPPAPGALEVASAEKRELGNVSASPYRANRLIIKNGEMNLLVANTDRAIDQV
                     TDIAVNSGGYIVSSRTWFDGEFKFATLTMGVPVDQFEAVQRQLRAIAATVLSDTASGQ
                     DVSEEYVDLQSRLTNLEATAARIREFLDQTKTVEEALQVNARLTEVENEIEQIKGRMN
                     YLKDRAAFSTIVVNLEPQRPTPTPTPTPTPVAWMPGKTVEAATGTLGNILRALGDMLI
                     WVVVVVGPFAVPAVIVGVIVVRGRKKNVKRDT"
     gene            complement(4313..>4909)
                     /locus_tag="AC162G_04845"
     CDS             complement(4313..>4909)
                     /locus_tag="AC162G_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR5075174.1"
                     /translation="YRLPEGFDDLQAAPLLCGGVIGYRALRLSQIEPGQRLGLYGFGA
                     SAHIAIQVARHWNCEVFVFTRSEEHRRHARRLGAAWAGGAEDTPPAPMDSSIVFAPAG
                     SLVPEVLRVLRPGGTLALAGITMSDIPALPYELIYRERTLRSVANATRRDADELLRLA
                     AEIPIHTDVEVMPLDRANEALLRVKRSDVSGALVLTTS"
BASE COUNT          778 a         1673 c         1496 g          962 t
ORIGIN      
        1 tccgtcacga tattccagtc gtatttccgc ttgaaaccgt cgatcgcgcc ctgcggccag
       61 tcggacgcgc cgacggcgcg cccgacgccc tcgaagatga tcggcgacga gccgtcttcc
      121 agggccaacg caacccgatc gttccgcgtc atgttccgcg ccttgacgct gttcggatcg
      181 atacagatgt agacctttcc gtcgtgccag acaaaccaca ccggcgtgag gtgcgggcgg
      241 ccatccggcc gcacggtggc gacccagatg ttgctctcgg attgcaggcg gtgttcggtg
      301 cgctgatcga tgcgtttcat gcttggctcc tggagccaat tataaccctc gtcacgcatc
      361 acgcttcacg tttcacgccg cctctgccat gcgctcctca ctcacgaagg tcaacccatc
      421 ctcgttcgca tcgaccgaga cgacgtcccc ttctccgtac acgccgctca gaatcttcag
      481 cgccagaggg tcttgcagct cacgctggat cgtgcgcttg agcggccgcg cgccgtagac
      541 cgggtcgaac cccttgtcgg cgagccaatg tttcgcccga tcgctcaact gcagatcgat
      601 cttgcggtcg gccaagagcc ggcgcaggct gaccagttga atctcgacga tgcgcgccag
      661 atcgtcgcgc gtcaggttgt tgaagatcac gatctcgtcg acgcggttca ggaactccgg
      721 tttgaagctc tgctccatgg cggccatcac acgccggcgc atctcggcgg gatcgccgcc
      781 gcccagctcc ttgatccact gcgaaccgac gttcgaggtc atgatcacga gggtgttttt
      841 gaaatcgacc gtgcggccat gcccatcggt caggcggccg tcgtcgagca gttgcagcag
      901 cgcgttgaat acctcgggat gcgccttctc gatctcgtcg aacagcacga cgctgtatgg
      961 cctgcggcgg atggcctccg tcaactggcc gccctcatcg tagccgacgt agcccggcgg
     1021 cgcgccgatc aggcgcgaga cggtgtgctt ctcctggtac tcgctcatgt cgatgcgcac
     1081 catcgcgctc tcgtcgtcga acaggaacgc ggccagcgac cgggccagct cggtcttgcc
     1141 gacgccggtc gggccgagga agatgaacga gccgatcggc cggctgcgat cctgcaggcc
     1201 ggcgcgcgcc cggcgcacgg cattcgacac gacgcggatc gcgtcgtctt gcccgaccac
     1261 gcgcctgcgt agccggtctt ccatgtgcag gagcttctga acctcacctt cgagcaggcg
     1321 gctcaccgga atgcccgtcc acttggcgac gacctcggcg acgtcttccg cctcgacctc
     1381 ttccttgagc atgcgctgcc ccttctgcag ctccttgata cgcgcctcgc cttcggcgag
     1441 ctgcttctca aggtcgcgca gcgtgccgaa gcgcagccgg gccgccgttt cgaggtcggc
     1501 gctccgctcg gcgacttcga tctcgtgccg ggtcgcttcg atcttctcct tgatcgcccg
     1561 cagcgaggtg atcgcgtttt tctccagttc ccaatgcgcg cgcagcgcgc tagattcttc
     1621 cttcagatcc cccagttcct tctcgatttt ctccaggcgc tctctgctag ccttatcccg
     1681 ctccttcttc agagcctcac gctcgatctc cagttgcatg atcttgcggt cgacctcgtc
     1741 gagcgcggcg ggcttggaat cgatctccat gcgcagccgc gacgcggctt cgtcgatcag
     1801 gtcgatggct ttgtccggga gctgccggtc ggtgatatag cggttgctca gcgtcgccgc
     1861 cgcgatcacc gccgaatcct ggatgcgcac gccgtgatgc acttcgtagc gctctttcag
     1921 gccgcgcagg atgctgatcg tctcttcgac gcccggctcg tcgacgtaca cgggctggaa
     1981 gcggcgctcc agggcggcgt ccttctcgac gtgcttgcga tactcgtcga gcgtcgtcgc
     2041 gccgatagtg tgcagctcgc cgcgcgccag catcggcttg agcatgttgc tggcgtccat
     2101 cgcgccctcg gcggcccccg cgccgacgac cgtgtgcagc tcgtcgatga agaggatcac
     2161 ccgcgtcgaa tcggcgactt ccttgagcac ggccttcagg cgctcctcga actcgccgcg
     2221 atacttcgcg cccgccacga gcgctcccat gtcgagcgcg acgatgcgct tgtccttcag
     2281 cccttccggc acgtcgccgc gcacgatgcg ctgcgccagt ccctcggcga tggcggtctt
     2341 gccgacgccc gcctcgccga cgagcaccgg gttgttcttc gtgcgccgcg agaggatctg
     2401 aatcacgcgg cggatttcgt cgtcacggcc gatgaccggg tcgagcttgc cctggcgcgc
     2461 caactgcgtg agatcgcgcc cgtacttttc gagcgcctgg taggtggact cgggatgctg
     2521 cgacgtgacg cgcgtcgatc cgcgcaccga gacgagcgcc tgcagaatcg cctgcgcgct
     2581 gacgccgcgc gcgttcagca tgctgccgag cgccgtgcgc gatcgggcca gcgcgatcaa
     2641 gatgtgctcg gtgctgacga agtcgtcgcg catcgacctg gcttcattct ctgcggcgct
     2701 gagcacgtca tttgccgcgc ggctcaaccc gacctgggta ttcgaccccg aaacgcgcgg
     2761 cctgccctgc aggtcacgct cgacgatggc ggcgatttgc tcgggccttt gtccgagctt
     2821 ctggagcaat tgcggcgcga cgccctcgcc ctggcgcaga agactcagca gcaggtgctc
     2881 gggttcgatg gcctggtggc cgaattcctc cgcgagcctc tgcgatccaa gaacggcttc
     2941 ttgcgctttt tcggtaaact tgttcagatt catcacctgc ctccaatcca aaaatataga
     3001 caataaaacg tgaggtactg gaccttgcga aggttgatgg gccttcgcaa gggttctaag
     3061 acaagtgcca gccaaattgt atctggctgg cactagagat gtattggagc agtattagat
     3121 ttgtgctgga ggctctgatc acaacgaggg cgatcggaac ccggtcgccc attacgtttt
     3181 acgtatcacg cttcacgttc ttcttccgcc cgcgcacgac gatcacaccc acgatcaccg
     3241 ccggcacggc gaatggcccg accacgacga cgacccagat cagcatgtca cccagcgcgc
     3301 gcagaatgtt gcccagtgtg ccggtcgcag cctcgaccgt cttccccggc atccaggcca
     3361 cgggtgtggg tgtcggcgtc ggcgtcggcg tcgggcgttg cggctcgagg ttcacgacga
     3421 tggttgagaa cgcggcgcga tctttcaggt agttcatgcg acccttgatc tgctcgatct
     3481 cgttctccac ctcggtcagg cgggcattca cctgcagcgc ttcttcgacg gtcttggtct
     3541 gatccaggaa ctcgcggatg cgcgcggccg tggcttcgag gttcgtcagg cgcgactgca
     3601 aatcgacgta ctcctcgctc acgtcctggc ctgatgcggt gtcgctcagc actgtggcgg
     3661 cgatggctcg cagttggcgc tgcacggcct cgaactgatc gaccggcaca cccatcgtca
     3721 gcgtggcaaa tttgaactcg ccgtcgaacc acgtccggct gctcacgatg tagccgccgc
     3781 tgttgacggc aatgtcggtc acctggtcga tcgcgcgatc cgtgttggcg acgagcaggt
     3841 tcatctcgcc gttcttgatg atcaggcggt tagcccgata cggcgacgcg ctaacgttac
     3901 ccagctcacg cttttcagca gaagccacct ccagcgcgcc tggcgccggt ggtgctgccg
     3961 tcactatcac cgcttcgggc aagcctgctc catctgccgc gttgaagcgt ggtgccggag
     4021 ttgccgctgg ggcggcgcaa gcagcagcta tgaccgcgac ggctgtcagc gtgcaaaaca
     4081 acgcaagctt cgtcaaggtg ttcatgttcc tctccttcct accacaaaga gccggctcgg
     4141 cgccggtccg gctcgcgacg tcctcctgcc ggtaggacgc ctgtgggcat tggcatgttc
     4201 ccgttctcaa cagaatggcc ccatgccacg cggcatgggg ccggtccagg gcgaccacca
     4261 ggatcgcccc tacaggaatc cgctgtcatg agggcggccg ccggggtcgc ccctacgatg
     4321 tcgtcaatac caaagcgcca ctcacgtcgc tgcgtttgac ccgcagtaac gcctcgttcg
     4381 cgcggtcgag cggcatcact tccacgtccg tatgaatcgg gatctccgcc gcgaggcgca
     4441 gcagctcgtc ggcatcgcgc cgcgtcgcgt tcgcgacgct gcggagcgtc cgctcgcgat
     4501 agatcaactc gtacggcagg gccgggatgt cggacatggt gatgccggcg agggcgagcg
     4561 tgccgccggg tcgcagcacg cgcagcacct ccggaacgag cgagcctgcc ggcgcgaaga
     4621 cgatcgagct atccatcggc gcgggtggcg tatcctccgc gccaccggcc cacgcagcgc
     4681 ccagccgccg ggcgtggcgg cggtgctcct cgctgcgcgt gaagacgaag acctcacagt
     4741 tccaatgccg cgccacctga atggcaatgt gcgccgacgc accgaagccg tatagtccca
     4801 agcgctgtcc aggctcgatc tggctgagtc gcagtgcacg gtaaccgatc accccgccac
     4861 acaacagtgg cgcggcctgc agatcgtcaa agccctccgg gagccgata
//
LOCUS       DBNHXB010000127         5616 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3883289, whole genome shotgun sequence.
ACCESSION   DBNHXB010000127 DBNHXB010000000
VERSION     DBNHXB010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3883289"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="AC162G_04850"
     CDS             complement(<1..756)
                     /locus_tag="AC162G_04850"
                     /inference="COORDINATES: protein motif:HMM:NF016176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR5075175.1"
                     /translation="MTLKQILLGVVIVAALGGAGLFILDNTLFAPVPVNTGLPVAPTL
                     PAPTTPPTEPPANPTSAAPVDRPGAAPTEPPAAPEASGARLYRIDAAQSEVRYEVGET
                     LFRNNRFQTAIGRTKGVAGDVLVDFAQPSNSQIGEIVIDVSQFTSNETRRDNYIRRDG
                     LQSSQYPYARFVPQSIEGLPDQVAPGEDLTFTIAGDLTVKETTLPVTWIVTLRVEEDR
                     LVGSAASEILMSDFGVGPIQVATLVTEDLVKLHF"
     gene            1188..2162
                     /locus_tag="AC162G_04855"
     CDS             1188..2162
                     /locus_tag="AC162G_04855"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006940256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR5075176.1"
                     /translation="MTDFKGYLLDEFLEDYQEGHLTRREALKLIGAVTGSLALANVIL
                     AACTPAPQAASTAVPTSTSAPAATSTPEPTSAPPEPTATSTTEATPPPSGEIEASDVE
                     FPGQDVTLMGYLARPVGDGPFPAVLVCHENRGLTDHIKDVTRRVATAGYVGLAVDLLS
                     RQGGTAAIGDSSQVPGVLGNTPEDQFVQDFVSGWRWLQTQPYVQPDRVGMVGFCFGGG
                     VTWRVAIGLPELRAAVPFYGPHPSPDEVPNIQAAVLAIYAGNDTRINQSIPAIEAAMQ
                     QSSKVYETVIYPNTDHAFHNDTGARYNAEAAQDAWARTLAWFEQYLKS"
     gene            2316..2996
                     /locus_tag="AC162G_04860"
     CDS             2316..2996
                     /locus_tag="AC162G_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5075177.1"
                     /translation="MATILIVDDDRKITDMLRRTLTYEGYRVATAADGREALAQAQAE
                     LPDLVVLDWLMPGPDGLEVAKRLRATSETRILMLTARDAVGDRVEGLDSGADDYLVKP
                     FAPEELLARIRALLRRSDPVTEDSPVTYADLHLDPVTRETRRGSRSFYLTPKEFDLLC
                     YLMRHPRQVLPRDRILQEIWGYDFDGNGNVLEVYIGYLRAKTEADSEPRLIQTVRGVG
                     YVLRENPD"
     gene            2998..4383
                     /locus_tag="AC162G_04865"
     CDS             2998..4383
                     /locus_tag="AC162G_04865"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5075178.1"
                     /translation="MSLRLRLTLLYSGILALTLIAFSVVLYVTVSQVTLNAVEETLAD
                     EAQRLIGSRQFQIDHIDYQARKVAAPETYIQTLKPDGAIADRTANLGDYVLPISTSGL
                     SACKGGRSWTETTSTEDGRLIVYSKPVIAQGEIAGIVQVARSLADHDHSLAALRSILI
                     AGSVVVTLTAFGVGWVLAGAALRPIHRITDTAQAIGAERDFDRRVDYTGPPDEIGRLA
                     KTFNAMLTELQATHRQTEQALQAQRRLVADASHELRTPLTTIRGNLGLLRREPPIRGD
                     DRQAVVADMVDETDRLIRLVNDMLLLARSDASRPLRIEAVRIGPLVEDLCRQARLLGV
                     GRLIECENVPDVVAAADRDALKQVLLILLDNALKYTPARGAITLYTSADEAHVTISIR
                     DTGPGIAPDALPHIFERFYRSEPSRTGSGAGLGLAIAKELIEAQHGTITVESQVGQGS
                     VFRVTLPRREG"
     gene            4474..4662
                     /locus_tag="AC162G_04870"
     CDS             4474..4662
                     /locus_tag="AC162G_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075179.1"
                     /translation="MIEWKVGDRFVLRNGRAVGTVIGVSRDMITVQYDDGRIDKVDPL
                     DLLRNIKPPPDPDEEDED"
     gene            complement(4770..5096)
                     /locus_tag="AC162G_04875"
     CDS             complement(4770..5096)
                     /locus_tag="AC162G_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075180.1"
                     /translation="MRQGERRRKSKTLWVLILLALVYSVLLGCQCVSTGTATLDGAIG
                     VMLGLYVCSHPAANAVDSLLFGRGVPRVSSKQLDVLWLVLNLLALLTGWLVIFIGAMR
                     FTANTA"
     gene            complement(5263..>5616)
                     /locus_tag="AC162G_04880"
     CDS             complement(5263..>5616)
                     /locus_tag="AC162G_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5075181.1"
                     /translation="ISLVVGGIGVMNIMLVSVTERTREIGLRKAVGARRGDILYQFLT
                     ETLTLSVLGGVIGILAGAGVSSLVNASGLIDTVVSIESVVLAFGFSAAVGIFFGIYPA
                     NRAAGLNPIEALRYE"
BASE COUNT         1122 a         1756 c         1738 g         1000 t
ORIGIN      
        1 aaagtgtagc ttgaccaggt cttctgtcac cagcgtcgct acctggattg gccctacgcc
       61 aaagtccgac atcaggatct cgctcgccgc cgaaccgacc agccgatcct cttccacgcg
      121 caacgtcacg atccacgtga ccggcagcgt cgtctccttg acagtcaaat cgccagcgat
      181 ggtgaaggtg aggtcctcgc ccggtgcgac ctgatcgggc aatccttcga tcgactgcgg
      241 gacaaagcgc gcgtagggat actgcgacga ctgcaagcca tcgcggcgga tgtagttgtc
      301 gcggcgcgtt tcattcgacg tgaactgact cacgtcgatg acgatttcgc cgatctggct
      361 gttggacggc tgcgcgaaat cgaccagcac atcgccggcg acgcctttcg tccgcccgat
      421 tgcggtctgg aatcggttgt tccgaaagag cgtttctccc acttcgtatc gcacttcaga
      481 ctgcgcggcg tcaatgcgat acaggcgcgc gccggatgct tcgggcgctg caggcggctc
      541 agtcggtgca gcgcctggtc gatcgaccgg cgccgcagat gttgggttag cgggtggctc
      601 ggtcgggggt gtcgtgggcg cgggaagcgt cggcgcgaca ggcagcccgg tattgaccgg
      661 caccggagcg aagagggtgt tgtcgagaat gaacaatcca gcaccgccca gcgcggcgac
      721 gatcaccacg ccgagcaaaa tctgcttgag tgtcaccgta tgtctccgtt catgtaacta
      781 ctcagcgtca tggcgacccc gctgagaagc gcggggcagg tgagcgtcgt tggcgacgaa
      841 gtaatctcag ccgccctgag cgcacatggc cgtccgtgca ggagattgct tcgcaaaaag
      901 cgctcgcaat gacgtgggct gagtagttac cgtttgatga gatgccaact acacaacggt
      961 agtcatcgta cccggtttgc ataaacgccg actaagcaca acctgagaat acgctgagaa
     1021 ccggatggcg ttctcagcgt tttcttagca ttcactcaga caatgcttaa tcgtacagtc
     1081 tacaatacta acatacaact cttattcgtt gagtcattct gagcgcttcc ttcgactgcg
     1141 ctcagggcaa ggctcagaat gacatgctca gtcagggagg tgctgcgatg accgacttca
     1201 agggttacct gctcgatgaa tttctcgaag attatcagga aggccatttg acacggcgtg
     1261 aggcgttgaa actgatcggc gccgtcaccg gcagcctggc gttggcgaat gtcatcctgg
     1321 cggcgtgcac gcctgcgccg caggcggctt caaccgctgt gccaacctcg acgagcgcgc
     1381 cggccgcgac atcaacacct gagccaacct cggcgccgcc tgagccaacg gcaacctcaa
     1441 ccaccgaggc aacgccgccc ccgagcggcg agatcgaggc gagcgatgtc gagttcccgg
     1501 gacaggatgt gacgcttatg ggttacctgg cccggcccgt tggcgatggg ccgtttccgg
     1561 cagttctcgt atgccatgag aatcgcggcc tgacggacca catcaaggat gtgacccgcc
     1621 gcgtagcaac ggcaggctat gtcgggctgg cggtcgactt gctctcgcgc cagggcggta
     1681 ccgcggcgat cggcgactcg tcgcaggtgc ccggcgtgct ggggaacacc cccgaggatc
     1741 aattcgtgca agacttcgtc agcggctggc gctggcttca gactcagcct tacgttcagc
     1801 ccgatcgagt cgggatggtc ggtttctgct tcggcggcgg cgtgacgtgg cgtgtggcga
     1861 tcggcttgcc ggagctacgc gcggccgtgc ccttctacgg ccctcatccg tcgcccgatg
     1921 aagtgccgaa catccaggcg gcggtgctgg cgatctacgc ggggaacgac acgcgcatca
     1981 accagagcat tccggcgatt gaggccgcga tgcagcagag cagcaaggtg tatgagacag
     2041 tgatctaccc caacaccgat cacgcttttc acaacgatac cggcgcgcgt tacaacgccg
     2101 aggcggcgca ggatgcgtgg gccaggacgc tggcctggtt cgagcagtat ttgaagtcgt
     2161 gatcgggcgg ggtataatag gtcgtagtaa cgacttcagt cgtttgccaa gaaagcgact
     2221 gaagtcgcca ctacaatggg ccgtagtaac gacttcagtc gtctgccaga aagcgactga
     2281 agtcgccacc acacagcagc acggcaagtg ggttcatggc gacgatcctg atcgtagacg
     2341 acgatcgcaa gatcaccgat atgctgcgtc gcacactcac gtacgagggg tatcgcgtgg
     2401 cgacggcggc cgacgggcgc gaagcgctgg ctcaggcgca agccgagctg ccggacctcg
     2461 tcgtcctcga ctggctgatg cccggccccg acggtctgga agtggcgaag cggctgcgcg
     2521 cgacgagcga gacgcgcatc ttgatgctga cggcgcgcga tgcggtcggg gatcgcgtgg
     2581 aagggctgga cagcggcgcg gacgattacc tggtcaagcc gtttgcgccc gaagagttgc
     2641 tcgcgcgcat ccgcgcgctg ctgcgccgat cggatccggt gacggaagac agccctgtga
     2701 cctatgccga cctgcacctc gatcccgtca cgcgcgagac gcggcgcggc agccgatcgt
     2761 tttacctcac gcccaaagag ttcgatctgc tctgttacct catgcgccac ccgcgccagg
     2821 tgctgccgcg cgatcgcatc ttgcaggaaa tatggggcta cgacttcgac ggcaacggga
     2881 acgtcctcga agtctacatc ggctatctgc gcgccaagac cgaggcagac agcgaacccc
     2941 gcctgatcca gaccgtgcgc ggcgtcggct acgtcttgcg ggagaacccc gattagcatg
     3001 tccctccggc tccggctcac tctgctctac agtggcatcc tcgcgctgac gctgatcgcg
     3061 ttcagcgtcg tgctgtacgt gacggtttca caggtcacgc tcaacgcggt cgaggaaacc
     3121 ctggccgacg aagcgcagcg actgatcggc tcccggcagt ttcagatcga tcacatcgac
     3181 tatcaggccc gcaaggtcgc cgctccggag acgtacatcc agacgctcaa gcccgacggc
     3241 gccatcgccg atcgcaccgc caaccttggc gattacgtgt tgccgatcag caccagcggc
     3301 ctcagcgcgt gcaagggcgg cagatcctgg acggagacga cgtcgaccga ggatggccgg
     3361 ctcatcgtgt acagcaaacc cgtgatcgcg cagggggaga tcgccggcat tgtgcaggtg
     3421 gcgcgctcgc tggccgatca cgaccattcg ctcgccgcgc tgcgcagcat cttgatcgcc
     3481 ggcagcgtgg tcgtgacgct cacggcgttc ggcgtgggct gggtgctggc cggcgcggcg
     3541 ctgcgcccga ttcaccgcat caccgacacg gcgcaggcca tcggcgcaga gcgcgatttc
     3601 gaccggcgcg tcgattacac cggcccaccg gatgagatcg gccggcttgc gaaaacgttc
     3661 aacgccatgc tgaccgagct tcaagcaacc catcgacaga ccgagcaggc gctgcaagcg
     3721 cagcgccgcc tcgtcgccga tgcctcccac gagctgcgca caccgctcac gacgatccgc
     3781 ggcaacctcg gactgctgcg gcgcgagccg ccgatcaggg gcgatgatcg ccaggccgtc
     3841 gtcgccgaca tggtcgacga aacggatcgc ctcatccggc tcgtcaacga catgctcctg
     3901 cttgctcgat cggacgcaag ccggccgctg cgcatcgaag ccgttcggat cgggccgctt
     3961 gtcgaagatc tttgccgcca ggccagattg ctcggcgtcg ggcggttgat cgaatgtgag
     4021 aacgtgcccg atgttgtcgc cgccgccgac agggacgcgc tcaaacaggt gctgctgatt
     4081 ttgctcgaca acgcgctcaa gtacacaccc gcacgcggcg ccatcacgtt gtacacctcg
     4141 gcggatgagg ctcacgtgac gatcagcatc cgcgacaccg gccccggcat cgcgcccgac
     4201 gcgctgccgc acatcttcga gcgcttctat cgatcggaac cctctcgcac aggctcgggc
     4261 gctggcctgg gcttggcgat tgccaaagag ctgatcgaag cgcagcacgg cacgatcacg
     4321 gtcgagagtc aagtcgggca gggcagcgtg ttcagggtga cattgccacg gagagaaggg
     4381 tgaggatagc gtaattggtt attggtaatt ggtgattggg tgacattgcc acggagagaa
     4441 gggggagaat aagagacatc aagggagttc attatgatcg aatggaaagt cggtgaccgc
     4501 tttgtgttac ggaacgggcg cgccgtcggc acggtgatcg gcgtcagccg agacatgata
     4561 accgtccaat acgatgatgg ccggatcgat aaggtcgatc cgctggatct tctcaggaac
     4621 atcaaaccgc cgcctgaccc cgatgaagag gatgaggact aagagtccca tcgctcaagc
     4681 aggctgttct tggatgagct gacggtcatc ccttcacgcg cgcggccaac atctcgatga
     4741 gccggtggcg ggtccatcga gaaactgttc taagccgtgt tcgccgtgaa tcgcatggcg
     4801 ccgataaaaa tgaccagcca tccggtcagc agggccagca ggttcaacac cagccacaat
     4861 acatcgagtt gctttgatga aactcgagga acaccgcgcc cgaacaacaa actatccacg
     4921 gcattggcag ccgggtgcga acagacgtac agaccgagca tcacgccgat cgcgccgtcc
     4981 agcgtagccg tgcccgtgga tacgcactga caaccgagca ggacactata aaccaaagcg
     5041 agcaggatga ggacccacaa cgtcttgctt ttgcgtctac gctcgccctg tctcatgtag
     5101 ctgtcacatt gatttccctg gggacatttg ttggctccgg aattggcagg tatcccgctg
     5161 ccgacttagt ccattgcgag agcatgctgc accttgaccg tgccgtaata cttgccagtc
     5221 cgtgtaatcc gtgggaaatc cgtgtgtcag gtcgcgatca ccctactcgt agcgcagcgc
     5281 ctcgatcggg ttgaggccgg ccgcccgatt ggccgggtaa atcccgaaga agatgcccac
     5341 cgccgccgag aacccgaagg ccagcacgac ggactcgatc gacacgaccg tatcgatcaa
     5401 ccccgacgca ttcaccagcg acgagacgcc cgcaccggcc aggatgccga tcacgccgcc
     5461 cagcaccgac agcgtgagcg tctccgtcaa gaactgatac aggatgtctc cccgccgcgc
     5521 gccgaccgct ttgcgcaagc cgatctcgcg tgtccgctcg gtgacggaca ccagcatgat
     5581 gttcatcacg ccgatgccgc ccaccaccag cgagat
//
LOCUS       DBNHXB010000128        26456 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3380330, whole genome shotgun sequence.
ACCESSION   DBNHXB010000128 DBNHXB010000000
VERSION     DBNHXB010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 26456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 26456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26456
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3380330"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1783)
                     /locus_tag="AC162G_04885"
     CDS             complement(<1..1783)
                     /locus_tag="AC162G_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR5075182.1"
                     /translation="MSVTEARTGPSPSGVKDRGLIVNDFSITAATVNGSGSQTANNTL
                     IRAIHKMGVPVSGKNLFPSNIQGLPTWFTIRVSKDGYIARRERAEILVAMNKATIAED
                     IQKLESGGICLYPIDDPLPVQRDDVAFYPMPVNELAKNSGADARLRPYVANMVYVGTL
                     AYLLGIDLKEIEAAIAFHFDGKKKPIDMNMGVVNAAHEWSSTHLTKTDPFRIDRMDAT
                     DGTFLIDGNTTAALGAIFGGVSFAAWYPITPATSLADALNEYLPKLRREPDGKATYSI
                     VQAEDELAAIGMVIGAGWAGARAMTSTSGPGLSLMAEFAGYAYFAEVPAVIWDIQRMG
                     PSTGLPTRVSQGDIEEAYYLSHGDTRHVCLIPGNVQECFEFGNTAFELAERLQTLVIV
                     LSDLDLGMNMWPTGPLKYPEKPMDRGKVLSAEDLKRLGGSWKRYADADGDGIGWRTLP
                     GTDHPMAAYFTRGTGHDEAANYSERPEDWQQNLERLARKHETARTLAPPPIIDMVEGA
                     EVGIISYGSNDPAVKEARDRLAARDIRTSYLRLRALPLEHTLTEFVKKHARLYVVENN
                     FDGQMHDLIQLHTPEYAARLVSISKCDG"
     gene            complement(2024..2497)
                     /locus_tag="AC162G_04890"
     CDS             complement(2024..2497)
                     /locus_tag="AC162G_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR5075183.1"
                     /translation="MAKTELFRVIARTIIVTPGRRLVLVTSHNSTALVLPGGAVDQGE
                     SLPQAAIREAKEECGLDVTVERAIWVREFHDLKRGQANLEVYFTAQPVAGAAPPDRWQ
                     HSDPGRPGLTRQVGLYSREDLQAIPTSVYPIELRRAFWIGLEEGFEDAYLGSFRG"
     gene            complement(2516..2959)
                     /locus_tag="AC162G_04895"
     CDS             complement(2516..2959)
                     /locus_tag="AC162G_04895"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5075184.1"
                     /translation="MDVEIVRASWRDAWALSRLDRRCFSQVDAYAWLTYLSLCLWPSI
                     VTLKAVSGEKIVGVVAGDPRRRRGYTLIVTLAVDPDWRRRGIGERLMRACEACFDLPR
                     FRLQVRKSNAPAIRLYQRLGYAIVDALPGYYGDGEDAYVMEKANR"
     gene            complement(3194..4123)
                     /locus_tag="AC162G_04900"
     CDS             complement(3194..4123)
                     /locus_tag="AC162G_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014347029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR5075185.1"
                     /translation="MEVVLGVILFALLALLVGLVVSMIRIVPEYQRLVVFRLGNCIGQ
                     RGPGLILLYPFGIDRAITVDLREEVREVPHQTNITKDNASIGIDFLIYWQVVDAVKSV
                     LEVNNFRQAALGIATTTLRAVIGDIALDDVLAQREHINQVLRAKLDEVTERWGVKVTA
                     VEIREIVPPREIQEAMTRQMSAERTRRAVVTEAQGQREAAITVAEGQKQSAILRAEGE
                     KQSAILQAEGQRTAQVLVAEGFAVALDRVYAVAKTVDAKTLSLQYLEALKAIGASPST
                     KFIFPMELTTMLGSLAGYTETAFLKQDGAQQAG"
     gene            complement(4154..4813)
                     /locus_tag="AC162G_04905"
     CDS             complement(4154..4813)
                     /locus_tag="AC162G_04905"
                     /inference="COORDINATES: protein motif:HMM:NF051402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR5075186.1"
                     /translation="MDFGQQLFEFLKDPNVAYVLLILGLWMLAIAVTTPGTGFAEVVA
                     ALALVGAAVGLFNLTVNFAGILLIVVAFTLFIVDVFSPTHGILTFGGVVSLVLGSLFL
                     FPGREVGAGVSGWLIGGAAVISTGMAGLVLHALARTRREKGVGMAVLQVVGAKGRVRK
                     ALVPGETGTAQIAGQLWTVEAGEPIESGAEVVVERAEGLKLYVRRAQSATAAPSTPQN
                     G"
     gene            complement(4918..5730)
                     /locus_tag="AC162G_04910"
     CDS             complement(4918..5730)
                     /locus_tag="AC162G_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075187.1"
                     /translation="MATDRSSLHSRSLRAMLVNALLDWRGAAVLAAALVLSVLLPSPL
                     PGWQTWYWWLAGIVGWLAISFAVFVNPNTGAQVVAEMLRVKFDPAAIKDGESRRAIEK
                     GLEYRAQIEAAANRARSGLLRDNLAETADEIDQWLENMYSLALRLDAFAVDRTIQQDL
                     HSVPSTIQTYEQRLRSVSDDKLRAQMHEIYEAKKAQWESLKNLQATMARARLQMDSAL
                     TALGTVYSQILLIGVKDIDSGRAQRLREDITEEIKSLHDVVAAMDEVYQYER"
     gene            complement(6009..6686)
                     /locus_tag="AC162G_04915"
     CDS             complement(6009..6686)
                     /locus_tag="AC162G_04915"
                     /inference="COORDINATES: protein motif:HMM:NF015945.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HIR5075188.1"
                     /translation="MASLLEKVQTLISANLHALVDSALKANSVAVMDEYIRQAENNLE
                     ALEDAAATVGGSVKTLKRKYEDYAAQVAKLDRDIDTLLTQNRDDLASAAQAKLNTTQR
                     LTEDYKEQWERQQTEYQNLMDARLKLEAKLTTIKQEREELLALLELAKSKELTIKTIK
                     SLDDVVGIGDADVARIGESIRARLDKAQAKSEMLASRLDEQMDEVLEKESLDNQLAER
                     KKRLGLS"
     gene            complement(6859..7968)
                     /locus_tag="AC162G_04920"
     CDS             complement(6859..7968)
                     /locus_tag="AC162G_04920"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR5075189.1"
                     /translation="MHSAQRILVVEDDVAMAGGICDVLELAGYEVRVAHHGHQALDEI
                     GKYSPDLIVSDIMMPGMDGYELFKKVRSNPDWVLIPFIFLTAKGQKSDIRLGKTMGAE
                     DYLVKPFDWEDLLVATRSRLDRAQALQQASQSEHNALKNRILNTLSHEFRTPLTYITG
                     YTNLLQEPDLTPEQLRQFLERLQSGSTRLSRLVEDFLFLVSLEAGEAHTAYMLQRDRF
                     SDWPAMIQRVLARYAEKAVAHKVTLSADVAADLPETTLHAAYIEDVVSRLVDNAVKFT
                     IGKEGRVQVSVTQEDAGILIGVADEGMGISEQDVLKIFQSFHQVDREMHEQQGAGVGL
                     AIVKGVVDLHGGRIDVKSRVGEGSTFSIWLPVLEE"
     gene            complement(8030..15463)
                     /locus_tag="AC162G_04925"
     CDS             complement(8030..15463)
                     /locus_tag="AC162G_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5075190.1"
                     /translation="MASPIRLLLVEPDNADRRRIHRALRGRTTRFRITDAPSLQDVPQ
                     RLRGARYDIALVDLEGWPRPFDGLDHLLHSAPDLPVAVLSRNDHAAEAIRRGACGFVA
                     KTEQDLALLHSTLAAVVEQAKERARTQAQLKQLQLSDALYRLLLDHVAEALLLCGSAG
                     DILHVNHAAEKLLDRPAQALIGAPFARLLAERPPWALSDWADSHPVRAVLNKPATPVE
                     VRVTPLALEDSSVFLVRLRDLQREERSERLLQALSRASAAMQGALTPQDVLRAAGEQL
                     RSIGLILSVFRYLDDEHAVVMIHTELGRRVLSQARRMLGFDPMAYHAPVDSSPVLQSI
                     LSRDSPVVLSDASQIVRELLPRRLKRLADPLAKLLNYEEQFGLRLSLAGETYGLIMVG
                     YPHGHLVPEDQPIIEAFAVQIQAALERSQRFEVTQSRLADQLQQLTRLVRVGEQMQLR
                     QPLDRLLDTICGAIGEGLKWGQVIVWLSEARSATMSPVAVRGVSPKSAAWPGGLDEKT
                     WRRSEYRIGRCYLVTGGDPGAQAWQSGDILVAPIEIGGALLGAIQLDRPQSGLRPAPD
                     DLTPLDLFANQAAVAIENAQLYVQAGEGLKRRVDNLTSLTTLSAVVDQGDLRASLEQA
                     LYQVLRVSGMDAIGISLLDLATGELQPYVWRGLSGAFWEEVKTSPTRIGEGVGGRALA
                     AGSAIVVTDVPTDPRIPYRDRLMQEGVQTVVGIGLAGRVPVGAMTLYAYEARRLGDET
                     LDWLTVAGRQIALSIENNRLIDAARRRQRMAEAVREVNAAVASNLELDSVLATILDQA
                     SRVVPHNSSCIMLAESNTLRVLAVRGLVDPGNVIGRAFPRDDRNPAWQAVVSREAQVV
                     ADVRAADGNSHGVPDGSARSWIGAPLIVRDKVIGLLALHHHQAGFYTEEDARNASLIA
                     QQAAVAIDNARRFEIEHERSERLRLLNDLGRELVVALDRDTIVQQAVKQMAGRFGYKH
                     VAVWWLDPALNELAPIARSDDRQHAPEAQSMAHLPVGCGIVGQAASTRQTYVTGDVDT
                     DPYYVVNPAGAGARSEIGVPLRIEERIVGVLDIQSDRSNAFSADDVIILETAASQIGA
                     ALAIAELYQQTQARAGNLSLLFAASQELGSSLDSDQVFGRLAQWIVTAVEATSARVYV
                     WDLQTNVGRLLAQYVGARASASERQSRVGVEQDLGGLSILVTAMKERRWTVYETTADH
                     DAIDAALREVLRAGVVHSALYLPLVVRERLIGYVEIWETGRVRSWTPDEAHVCQTIAN
                     VAASAIDNARLFEVERQRRKVAETVRELAAVISSSLEPQAILESLLDRAAELIPYDSA
                     AVFIAPGELSATRAERSGQLRIAVSRGMPAEAHDSPPGLAEEVLRTRRVSICADARAG
                     VERGAAHGPSALPPSGVAEPALSAAKGQALDHVRGWLGAPLVVKGSAIGVLTFDSRTP
                     ARYRREHAEIAETIANHAAVAIENAQLYQEARQRVAELETLQAVSLEMIQSLDARRVS
                     QAIADGALRLLAATVVHLFSFDAETDTVELLAFSTAPGFEEIGWPTPRREGMTMRIAH
                     SGQAVVVNDPQNDPAFAAITRATAEQGAQALVGLPLKVREKVLGVMNVLFHAPHIVVD
                     NEVRILGLLADQAATALENARLFEGEQKRRVAADVLREISGVLTSTLNLDELCERLFD
                     QIARVIPYTSATVTLVEASRHLRIIAGRGFQQPAQIIGRTLALGDASLSGRTIDAREP
                     LVVEDVQQLGNGEVLPGMERVHGWMGAPLIARDRVIGTLSVDHEQAGIYRREQADLLG
                     VIANQAAAAITNARLYEQALERERFASALGRTSLAIMSSLELDEVLEQICRESAGAFN
                     VDSGLVWLAEGDDLVGFAGYGPAREGFVGVHVGLGDASILGARVLVERRGEYINYAET
                     DPRANRELVHRMGLKSLLGVPMVQDDAPIGTLVVGDSVRPDPFSAADIERAQVLASHA
                     VIAIENARLFQAEQRRARQLALVHRVGLDITSILDLDQLAQTVVEEIRGAFGYYHVSV
                     VTIHGGELVWRAGAGGDVEGWTPAGMRRPVGVGIIGAVAVTGEMLVAPDVRRDPRYVP
                     VPEVQRTLSKVALPLRAKGSIVGVLNVESDHADAFGEEDLAVLTALASQLGVAVENAM
                     YATSLETRVAERTAEIRREQERTFTILNSVADAVLVTDLAGAIVLTNPVADALLREDQ
                     EAEQPDRLRTWLRELSPASGSPKIDAGGRTLQAAVAYIREDERRVGHVIVLRDITRME
                     EVDRLKTQFVTTVSHELRTPLTNIKLYLGLFQKGKPEKREQYLVTLQDEVNRLERLIM
                     DLLDLARLERDRQTATRKVIDLAEVLQHVVSTLEPQAEAKRQSLHLRLTEQALRVPAD
                     RNQMIQVFVNLCANAINYTPPDGRVELEASSMLRDGRNWAVVSVSDNGIGIPEEDRER
                     VFDRFYRGQAEYFEVRGTGLGLSIVKEIVVQHSGQIELESRVGLGSTFTVWLPMS"
     gene            complement(15703..17055)
                     /locus_tag="AC162G_04930"
     CDS             complement(15703..17055)
                     /locus_tag="AC162G_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5075191.1"
                     /translation="MSSPRIFRAADAEFILRTAAEGFSSAIAGLSNVGKSELLRTACS
                     PEVLAWALGERADEFVFIYVDCNLMLTLTAQGFYEVTLRAALDALRRIGAPGSIAAQL
                     DDLYRMIVQPPSEFAVPLAFNDAMDALSRNLDRNIVFLFDEFDEPFARLDSRVLLNLR
                     ALSDKFGRRLSYVVATTAPLSARSVDSDVSEFVELFVGQQRTLGMLSDGEARALASEW
                     AAVEGAALQPHEIDFVVEQAGGHAGMIQAVTRVVLALVTGAPGRARGQALALASQRLE
                     DDQVARAECSKLWSQLSDDERHALIDLVLDSSASIDPALLAALCEKGVLVGEPPRVFG
                     QHFAGFVRRQRRARQAAQSGVWVDVDAGEVTVDGRQAPTLTDLEYRLLLLLWGRLDKL
                     CDKYQIVESVWGQDYIDEVDDARIEKLVSRLRAKLETDPTNPRYLLTVRGRGYKLVGV
                     "
     gene            complement(17122..17730)
                     /locus_tag="AC162G_04935"
     CDS             complement(17122..17730)
                     /locus_tag="AC162G_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075192.1"
                     /translation="MTTEQMNATNGNGDESSNRTFIIAVGVMAVVLVLGIIVIVIALS
                     NRNGRAQQVADQTATALAVAQVSPTVEPTFTPEPTDTPAPTDTPAPTDTRAPTATPRA
                     TATAITTAIATGSPAAGTPAGAAAAARTATSTATRQSPATPPAALTATAAARRTPSAT
                     GTPGSGTGTMPGTGIGDVAGLALAGGLVVLLFIARRLRTSQT"
     gene            complement(17763..18845)
                     /locus_tag="AC162G_04940"
     CDS             complement(17763..18845)
                     /locus_tag="AC162G_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW motif-containing protein"
                     /protein_id="HIR5075193.1"
                     /translation="MPETTAYYPPETRVMPLTLIRRDRMLPVPGNILVGPGQRVEASD
                     VVAQVELGGQFLTFEIARLLRVPPARAVRYLKKGKDDVVKAGEVIAGRMGLGVRPVRS
                     PVDGVIVDVDERDGRVAIEVASRLFELRAYVKGVVSNMLGHLGVVIETPGALIQAALG
                     IGGESFGVLRAVSEAPDEALRASSIDIRAHGSIVVGGGWITASALEQAQQLQLRGMIA
                     GSLEGDLLETARQMPFPIVVTEGVGRIGMATPIFELIRAQDGREASINGETRTRWGIV
                     RPEILIPLPAETRPPLPPAVGAPLTVGARVRVVRGPYMGQVGTVTAIPTRTVRIEIGT
                     RVHGADVRFSPASTAFVPFANLELLR"
     gene            complement(18915..20699)
                     /locus_tag="AC162G_04945"
     CDS             complement(18915..20699)
                     /locus_tag="AC162G_04945"
                     /inference="COORDINATES: protein motif:HMM:NF025309.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HIR5075194.1"
                     /translation="MDASSSQLDSIVAIDIGTVYTKAALIARVEGAYRFIARAESVTT
                     AEAPWSDVAVGARHALEKLSSAAGRRLLNERGDLTTPQQPDGSGVDACVVTASAGKPL
                     SVVLAGLAGEWSLESLRHAAMGTYTTLAAEIALSGGSRRAGRSIADDRIQRIYDARPD
                     VVCISGGTDGGAERPVLELVEAAKFAARFLEVAGSRPRILFAGNARLKPRVAETVGDA
                     ADFLQIDNVRPGLDVENVGPLQVELETMYTTHKMGRIAGLATISAWSKRSIMLPTARA
                     FGHVAQYLSAENRKTGVLGVDVGGSTTTVAAAFGGQLYTTLRSDVGASFVARFVEEIG
                     WESVARWLDFEVSEGDIRAFAANKELRPGTIPQDTSELRIEQALAREVIRATLRAARG
                     AWPHGTSIPRFSDSHVPLFEPIIGAGGMLARAPRPGQAALLMLDAIEPVGVTTLVLDV
                     YGLAAALGAAALAEPLIAVQALEQGALMHLATVVAPLGAMRARAGEVVMNVRLSYESG
                     GTIEDDIKAGSLSVLPLSPGQKAVMQLRPRVGIDIGRGPGRGGKPTEITGSALGLIVD
                     ARGRPLGVSPDPKRRRDQIKSWLWDVGA"
     gene            complement(20709..21359)
                     /locus_tag="AC162G_04950"
     CDS             complement(20709..21359)
                     /locus_tag="AC162G_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075195.1"
                     /translation="MQPETIQIVVGAVLTFAVLSYLIGDNAAYRLAMHLLVGVGAAYA
                     VGVAIGQVLAPRMLVPLARPADGIGPLVFGLFGLLGCVFLLAKIFPRTAWLGNVAGGT
                     MLGVGVGVAVGGALFGTLLPQTSAAAETAAGSLRSREAGDIVIGLLLLASTVTTLLAF
                     TYRPASRRAPLGVMGLIGQGFLYVALGATFALVFISGASMLVAWVRDLVSRVPAGG"
     gene            complement(21500..24106)
                     /locus_tag="AC162G_04955"
     CDS             complement(21500..24106)
                     /locus_tag="AC162G_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR5075196.1"
                     /translation="MIYCPNCGTANRDGSRFCNDCGHKLPSKTGIVCPMCSHMNPPAN
                     VYCDKCQARLVPAGPASPPVAPGAPAPPAPVKKGLSLPTKPADEIEPPPGEPETTPGL
                     EPEAEPEETPEWLHRLRAATPKPPPQAAPEFPSGEEIPEWMRPAEGEQLDWFDRLAGE
                     PPPSSPEKPAPAPVEEEPEAWMLDLASGSAMRQEATQAPEGEAGLPDWLKELTPPSAA
                     PADEPTLSRSPERSEGEAEEPALSGVEGSLDWLTAAAQEPQPGLGEPPVAGEIPDWLA
                     ELGLEAAAPEISERPSEPVEPPSPAPDTWHLEQGALSEQPQPAETGLAGEGHGWPADL
                     QAQPPAEEREPPAEDWRGWPPSTTLEEPSPTGEEPAGEVPDWLKDLGAGPIGPEEAGA
                     PAAAGAPPSGVEPPGDVSAFAGADIDTADLAATPDWLAQVQEPAREAPEQREEPAPSE
                     LPDWLREIGPMPALAPSETPFGEMPSPPGEARELIPPGEAQLADRLTTPAFADEQGRT
                     LTPDLEAAAIPSWLEALRPVAAPTGAAEEGVIETEGLLAGLQNVLPATPFIGKPQGAP
                     AARHAQVPPADIARAELFQRLLSTGALAPTAVKPETAGAQARLRANIVRWVIVVALVL
                     AAFAPNLDDFMAELGIFQLGSVDPALYAGAAAQIDSLDQGQRVLMSFDYDASQAGEMD
                     PIAEAFLVHIRQRGAQAIAVSLNPNGVALGARVVESLGEKGIPDAAFVQTSFHPGQAV
                     GAQSAILSVPADLIIVLAGSPEAMRWWVEQATFSGAEAPIVAGASAGALPQIQPYLQS
                     EQVDAVVQGLMGGLAYRRSLDPDQDAGEPGFDRLVRSEALYLSQIAFAAIILAGLIVS
                     LAAGRGRRPDAS"
     gene            complement(24109..24780)
                     /locus_tag="AC162G_04960"
     CDS             complement(24109..24780)
                     /locus_tag="AC162G_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075197.1"
                     /translation="MLFKRAFPVALTIAFAWLILISYLPLDPDSPAAAALLNVRGVLV
                     EWAVNLAAFALVLGFGNLIGIHARRIRSGEGVLYSVVLIGGALFTLGLWIAHILSVMA
                     ERQVPLTVAMQDPGFLVSAFDWVIAPLQSGLGILLAFVLALAGFRALQVRRSVGMVLF
                     VATAIVVALTQPVVPLLGVLGSFRAVVDSITTGSLRGLALGVALGSLAFGLRIIVGAD
                     KPQGD"
     gene            complement(24788..25540)
                     /locus_tag="AC162G_04965"
     CDS             complement(24788..25540)
                     /locus_tag="AC162G_04965"
                     /inference="COORDINATES: protein motif:HMM:NF042826.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HIR5075198.1"
                     /translation="MIIQLLLQIALVVIALSLPIAMLVFSTSARRARVPQMRPLSAFD
                     VLRGQVGRGIETGRPAHISIGVGGIVGDDTLVTLAGASIVDSLAPEAAETGAAPTVTV
                     ADGAAMILAQDTLRRPYARRGDLAGYDPRDVQLLGMQPFQYAAGAMDFLAHAQPVSNV
                     MVGAFGPEAGLIVEEGVKQGLTQVAGSTDARSLAVLFPSANHLLVGEEVFAAPAYLDQ
                     RPGHIARLQAQDLVRFIVAAVIVIWSILLLFA"
     gene            complement(25537..25863)
                     /locus_tag="AC162G_04970"
     CDS             complement(25537..25863)
                     /locus_tag="AC162G_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075199.1"
                     /translation="MISRNADRDEEQVVLWASRLRRWGVGDFVPLAVEVLRPFTFLGA
                     QALHLFTPILTTFASPAEISGLADLLESPEALDRLSERFALPGRGQGSDSREVAPAEP
                     DEEART"
     gene            26098..26313
                     /locus_tag="AC162G_04975"
     CDS             26098..26313
                     /locus_tag="AC162G_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075200.1"
                     /translation="MSKDEKLYGHLTGRRRWAIVLLGALSGLAIALIFAWFAGRSATM
                     VELVIGVVAGGLLGLSASLGEKRRRKT"
BASE COUNT         4655 a         9000 c         8238 g         4563 t
ORIGIN      
        1 gcccgtcaca tttcgatatc gatacgagcc gcgctgcata ttcgggcgtg tgcaactgaa
       61 tcaagtcatg catctgcccg tcgaagttgt tttccaccac gtacaggcgc gcatgtttct
      121 tgacgaactc tgtcaacgtg tgctccagcg gcagcgcgcg cagccgcaag taggacgtcc
      181 ggatgtcccg cgcggccagc cggtcgcgcg cttccttcac cgccggatcg ttcgagccat
      241 acgagatgat gccaacttct gcgccctcca ccatgtcgat gataggcggc ggcgcgaggg
      301 tgcgcgccgt ctcatgcttg cgcgccagcc gctccagatt ctgctgccag tcttcggggc
      361 gctcgctgta gttggcggcc tcgtcgtgac cggtgccgcg cgtgaaataa gcggccatcg
      421 ggtgatccgt gccgggcagc gtgcgccagc cgatgccgtc gccgtccgcg tccgcgtagc
      481 gcttccacga gccgcccagc cgcttgagat cctccgccga cagcactttg ccgcgatcca
      541 tcggcttctc gggatacttg agcggcccag tcggccacat attcatgccc aggtcgagat
      601 cgctcagcac gatgacgagc gtctgcaatc gctcggccag ttcgaacgcc gtgttgccaa
      661 actcgaagca ctcctgcaca ttgccgggaa tcaggcacac gtggcgcgtg tccccgtggc
      721 tgagatagta agcttcctcg atgtcgccct gggatacgcg agtcggcagc ccggtcgacg
      781 ggcccatgcg ctggatatcc caaatgaccg ccgggacctc cgcgaagtag gcatagcctg
      841 cgaactcggc catcaacgac aggcccggcc ccgaggtgct cgtcatggcc cgcgccccgg
      901 cccagcccgc gccgatcacc atgccgatcg cggccagctc gtcctcggcc tggacgatcg
      961 agtacgtcgc cttgccatcc ggctcccgac gcagcttcgg cagatactcg ttcagtgcat
     1021 cagccagcga cgtcgccggc gtgataggat accaggccgc gaatgatacg ccgccgaaga
     1081 tcgcgccgag cgcggcggtc gtgttgccgt cgatcaggaa cgtgccatcc gtggcgtcca
     1141 tgcgatcgat acggaaggga tcggtcttgg tcaaatgggt cgacgaccac tcgtgcgcgg
     1201 cgttgacgac gcccatgttc atgtcgatcg gcttcttctt gccgtcgaag tggaacgcga
     1261 tcgccgcttc gatctccttc agatcgatgc cgagcaggta ggccagcgtg cctacgtaaa
     1321 ccatgttggc gacgtagggg cgcaggcggg cgtcggcccc cgaattcttc gctagctcgt
     1381 tgaccggcat agggtagaag gccacgtcgt cgcgctggac cgggagcggg tcatcgatcg
     1441 ggtacaggca gatgcctccc gattcgagct tctgaatgtc ctcggcgatc gtcgccttgt
     1501 tcatcgccac caggatctcc gcgcgctcgc ggcgggcgat gtaaccgtct ttgctgacgc
     1561 ggatagtgaa ccaggtcggc agtccctgga tattcgaagg gaacaggttc ttgccgctga
     1621 ccggcacgcc catcttgtga atggcccgga tcagggtatt gttggccgtc tggctgcccg
     1681 atccgttcac cgtcgcggcc gtaatcgaga agtcgttgac aatcaggcca cgatctttga
     1741 ctccggacgg ggacggtccc gtccttgctt cggtaacact catctgtttc acctcacccg
     1801 agtaagccag atgcatctgg caaggtcgtc ataagcgaat ccgtgtaatt gtaccacctt
     1861 ccgctagatg cgtcattgac atttatgtct cattgcgaat ctcacaccgc ttgcagatac
     1921 ctgccagccg gcagaggtgc aggccgattc tctattcagc ccctgaagct cacgctgccg
     1981 gggacggcgg cttgagccta aaggagcaca ggccgattct tactcagcct ctgaagctcc
     2041 ccaaatacgc atcctcgaag ccctcttcca gcccgatcca gaatgcgcgc cgcagctcga
     2101 tgggatacac gctggtcggg atcgcctgca ggtcttcgcg agagtacagc cccacctgcc
     2161 gcgtgaggcc gggccggccg ggatcggagt gctgccagcg atcgggcggc gccgcgccgg
     2221 caaccggctg cgcagtgaag tacacctcca gattagcctg gccgcgcttc aggtcgtgaa
     2281 attctcgcac ccagattgcg cgctcgacgg tcacgtccag gccgcattct tccttcgctt
     2341 cgcgtatcgc agcctgcggc agcgactcac cctgatccac cgcgccgccg ggcagcacca
     2401 gcgccgtgct gttgtggctg gtcaccaaca ccaggcgccg ccccggcgtc acgatgatcg
     2461 tgcgcgcaat cacccgaaac agctcggttt tcgccatgct cgctcctcac ccttttcacc
     2521 tgttcgcttt ctccatcacg taggcgtcct cgccgtcgcc gtaatatccc ggcagggcat
     2581 cgacaatagc ataacccagt ctctgataca gccggatcgc cggcgcgttg ctctttcgaa
     2641 cttgcaagcg aaatcgaggc agatcgaagc aagcttcgca ggcgcgcatc aggcgctcgc
     2701 cgatgcctcg gcggcgccag tccggatcga cggccagagt gacgatgagc gtgtaaccac
     2761 gccgtctgcg cgggtcgcca gccacgacgc ccacgatttt ctcacccgag acggccttca
     2821 gcgtcacgat gctcggccag agacacaggc tgagataagt gagccaggca taggcatcga
     2881 cctggctgaa acatcttctg tccaggcggc tcaacgccca cgcatcgcgc caactggccc
     2941 gcacgatctc gacgtccaat cgcgtcccct ttcacgaaaa cgctctgagc ggcgtcctgc
     3001 aaacacgagg gccgagagcg cacatctcgg ctctcgaccc tcacgtcagg cgagtgggat
     3061 acacaaccgc ttagagccta tcggacaccg gatcgcaggc ttcagcctgc cctttctaaa
     3121 ggtgatccat ccgccgccct gaccgccgga tggatcacgc gaagcggagc gggagcggat
     3181 gcgaaatata ggcttagccc gcctgctgtg cgccgtcctg cttcaagaac gccgtttcgg
     3241 tgtagccggc cagcgatcca agcatggtcg tcagctccat cgggaagatg aacttggtgg
     3301 acgggctggc gccgatggcc ttgagcgctt cgagatactg cagcgacaag gtcttggcgt
     3361 cgaccgtctt ggcgactgcg tacacgcggt ccagcgcgac ggcgaaacct tcggcgacga
     3421 gcacctgtgc ggtgcgctgg ccctcggcct gcaagatggc cgactgcttc tcgccctccg
     3481 cgcgcaggat ggccgactgc ttctggcctt cggcgacggt gatcgccgcc tcgcgctgac
     3541 cttgcgcctc cgtgaccacc gcccggcgcg tgcgctcggc gctcatctgg cgcgtcatcg
     3601 cctcctgaat ctcgcgcggc gggacgatct cgcggatttc cacggccgtg accttcacgc
     3661 cccagcgctc cgtcacctcg tccagcttgg cgcgcagcac ctgattgatg tgctcgcgct
     3721 gcgccagcac atcgtccagt gcgatgtcgc cgatgaccgc gcgcagcgtc gtggtggcga
     3781 tgccgagcgc ggcctggcgg aagttgttca cctcgagcac gctcttcacg gcgtcgacga
     3841 cctgccagta aatcaggaag tcgatgccga tcgacgcgtt gtctttggtg atgttcgtct
     3901 ggtgcggcac ctcgcgcact tcctcgcgca gatcgacggt gatcgccctg tcgatgccga
     3961 agggatacag cagaatcaag ccaggccccc tctgaccgat gcagttgcct agccggaaca
     4021 ccaccagccg ctggtattcc ggcacgatac gaatcatgct gacgaccaga cccacgagca
     4081 gagccagcag ggcaaacaag ataacgccga ggactacttc catggtaagg cctccttggt
     4141 atgcatcaag atgtcatccg ttctgaggtg tcgacggcgc agccgtggcc gattgcgcgc
     4201 gccgcacata cagtttcaag ccctcggcgc gctccacgac cacttccgcg cccgactcga
     4261 tcggctcgcc ggcctccacg gtccacaact ggccggcgat ctgcgcggtg cccgtctcgc
     4321 cgggaacgag cgccttgcgc acccggccct tcgcgcccac tacctgcagc acggccatgc
     4381 cgacgccctt ttctcgccgc gttctggcca gcgcgtgcag caccaggccg gccatgcccg
     4441 tcgagatcac ggccgcgcca ccgatcagcc agccggatac gcccgcgccc acttcccgtc
     4501 ctgggaacag gaacagcgag ccgagcacga gcgagacgac gccgccgaag gtcaatatgc
     4561 cgtgcgtcgg cgaaaacacg tcgacgatga atagggtaaa ggcgacgacg atcagcagga
     4621 tacccgcgaa gttcaccgtc agattgaaga ggccgactgc tgcacccacc agcgcgaggg
     4681 cggccacgac ttcggcaaaa cccgtgcccg gcgtcgtcac cgcgatcgcc agcatccaca
     4741 gtcccaggat cagcagcacg taagctacgt tggggtcctt cagaaactcg aagagctgtt
     4801 gaccgaaatc catagcatcc tcccgctcag agacaactct aagcctcgct catctgcttc
     4861 gcgggcgcgg cgtgcgggcc gaagacgagc gtatggtatg cgactcgcac caacgcgtca
     4921 gcgctcgtat tgatacacct catccatcgc cgccacgacg tcgtgcaggc tcttgatctc
     4981 ttcggtgatg tcctcgcgca ggcgctgcgc gcgtccgctg tcgatatctt tcacgccgat
     5041 caggaggatc tgcgagtata cggtgccgag cgcggtcagc gcgctgtcca tctgcagccg
     5101 ggcgcgcgcc atggtcgcct ggagattctt cagagactcc cactgcgctt tcttggcttc
     5161 gtagatctca tgcatctggg cgcgcagctt gtcgtcgctg acgctcctca ggcgctgctc
     5221 gtaggtctgg atcgtcgagg gcaccgagtg gaggtcttgt tgaatggtcc gatcgactgc
     5281 gaaggcgtcc agccgcagcg ccagcgagta catattctcg agccactgat cgatctcgtc
     5341 cgccgtctcg gcgaggttat cgcgcagcaa gccggatcgc gcgcggttcg cggctgcctc
     5401 gatctgcgcc cggtactcca ggcccttctc gatggctctg cgcgattcgc catccttgat
     5461 cgccgccgga tcgaatttga cgcgaagcat ctccgccacg acttgcgcgc ccgtattcgg
     5521 attgacgaag acggcgaagg agatggccag ccagcccacg atacccgcca gccaccaata
     5581 ccaggtctgc cagccaggca acggggatgg cagcagcacg ctcaggacga gcgccgctgc
     5641 caacaccgcc gcgccgcgcc aatcgagcag ggcattgacc agcatcgctc tcaaagagcg
     5701 gctatgcagc gacgagcgat cggttgccat gcgcgtctcc acctgtgttc tgcttggaac
     5761 cgtttatagc ggtttccgat tgcgtgtacg ccgctccgga accgaggctt taggcggttc
     5821 ccggctaaag cctcgactcc ggcgtaagcc aaagcggaat ccgctctaga acccggcccc
     5881 gtctcaccgc gaagcgcaat ccgcattgtc cccttcgact gcgctcaggg ccggcctgac
     5941 gaaagggggc caccaccctc ggtgccggcc ccgctctgcg tcacgcctgg tggtggttgg
     6001 gcgttcgatc agctcaggcc caatcgtttc ttgcgctcgg ccagttgatt gtccagcgac
     6061 tccttttcca ggacctcgtc catctgctcg tccaggcggc tggccagcat ctcgctcttg
     6121 gcctgcgcct tgtcgaggcg ggcgcgaatc gattcgccga tgcgagcgac gtcggcgtcg
     6181 ccgatgccca ccacgtcgtc cagcgacttg atcgtcttga tggtgagttc cttgctcttc
     6241 gccagctcga gcagcgccag cagctcctcg cgctcctgtt tgatcgtcgt gagcttggcc
     6301 tccagcttga gccgggcatc catcaggttc tggtactcgg tctgctgccg ctcccactgc
     6361 tccttgtaat cctcggtcag gcgctgcgtg gtgttgagct tggcctgcgc cgccgacgcc
     6421 aggtcgtcgc gattttgcgt gagcagcgta tcgatgtcgc gatccagctt ggccacctgg
     6481 gcggcataat cctcgtattt gcgcttgagg gttttgacgc tgcccccaac cgtcgcggcc
     6541 gcatcctcca gcgcctcaag attgttttcg gcctggcgga tgtactcgtc catgacggcc
     6601 accgagttcg ccttgagcgc cgagtcgacg agcgcgtgta ggttggccga aatgagcgtc
     6661 tgcacttttt ccaacaggga tgccatggca ctgtcctcct caggagtgat caaattctgc
     6721 gtgacaggat actacccgga ctcacagcgt gcttgacggg cgtcggacac tcgcctgaca
     6781 gtccaatcat cagacctagc cgatcggatt ttcagacttc ggaagtctcc cgcttaaaga
     6841 cttccgaagt ctgggtggtt actcttcgag aacaggaagc cagatgctga acgtgctgcc
     6901 ttcgccgacc cggctcttca cgtcgatccg accgccgtgc aggtcgacga cacccttcac
     6961 gatagccagt cccactccgg cgccctgctg ttcgtgcatc tcccgatcga cctggtggaa
     7021 cgactggaag attttcaaca catcctgctc ggagataccc atgccttcgt ctgctacgcc
     7081 gatcaggata ccggcgtcct cttgcgttac cgatacctgc acccggcctt ccttgccgat
     7141 cgtgaacttg acggcattgt cgaccaggcg cgacactaca tcctcgatgt aggccgcgtg
     7201 aagggtcgtc tcgggcaggt ccgcagccac atccgcgctc agcgtcacct tgtgagcgac
     7261 cgccttctcg gcgtatcgcg ccaggacgcg ctggatcatg gcaggccaat ctgagaagcg
     7321 atcgcgctgg agcatgtagg ctgtgtgagc ctcgcccgcc tccagcgaga cgaggaatag
     7381 gaagtcttcc accaggcgac tcaaccgggt gctaccgctc tgcagccgct cgaggaactg
     7441 gcgcaactgc tccggcgtca gatccggctc ctgaagcaga ttcgtgtagc cggtgatgta
     7501 ggtgagcggt gtgcgaaact cgtggctcag ggtattgagg atgcggttct tcagggcgtt
     7561 gtgctcggat tgagacgctt gctgcagcgc ttgagcgcga tccagcctgg aacgcgttgc
     7621 gaccaagagg tcttcccaat cgaagggctt gaccaggtag tcttcggcgc ccatcgtctt
     7681 gcccaaccgg atgtccgatt tctggccctt ggcggtgagg aaaatgaaag ggatcagaac
     7741 ccagtccggg ttcgatcgga ccttcttgaa caactcgtag ccgtccattc ccggcatcat
     7801 aatatctgac acgatcaggt cgggcgagta cttgccgatc tcgtccaggg cctgatggcc
     7861 gtgatgcgcc acgcgcactt cgtagcccgc cagttcgagc acgtcgcaga tgccaccggc
     7921 catcgccacg tcgtcttcga caaccagaat gcgttgtgct gagtgcatcc gaaacctccg
     7981 cgctcaactc agaaccccgg tttctccaag aaaccggggt tcggcactct caactcatcg
     8041 ggagccagac cgtaaaagtg ctgcccaacc ccacccggct ttccaattcg atctgtccag
     8101 aatgctgcac gacgatttct ttgacaatgg acagccccag ccccgtcccc cgtacctcaa
     8161 agtactctgc ctggccgcga tagaagcgat cgaaaactcg ctcacgatcc tcctcgggta
     8221 tgccgatgcc attgtcgctc accgacacga cggcccagtt tcgaccgtct cgcagcatgc
     8281 ttgacgcttc cagctccacc cgcccgtctg gcggcgtgta gttgatagca ttggcgcaca
     8341 gattgacaaa tacctgaatc atctggttcc gatcggccgg cacacgcagc gcctgctccg
     8401 tgagccgcaa gtgaagtgac tggcgtttgg cttcggcctg cggctcgagc gtgctgacga
     8461 cgtgctgcag cacttcagcc agatcgatca ccttgcgggt cgccgtctgc cgatcgcgct
     8521 ccaggcgcgc caggtcgagc aggtccatga tcaggcgttc caggcggttg acctcgtctt
     8581 gcagcgtgac gaggtactgc tcgcgcttct ccggtttccc cttctggaac agtcccaggt
     8641 agagtttgat attcgtcagg ggcgtgcgca gctcgtgcga gaccgtcgtg acaaattgag
     8701 tcttgagccg atccacctct tccatgcgcg tgatgtcgcg cagcacgatc acgtggccga
     8761 ctcgccgctc gtcttcccgg atgtacgcca ccgccgcctg cagcgtgcgc ccgccggcgt
     8821 cgatcttcgg agagccgctg gcgggcgaca gctcacgcag ccacgtgcgc aggcgatccg
     8881 gctgctccgc ctcctgatct tcgcgcagca gcgcgtcggc gacgggattg gtcagcacaa
     8941 tggcgccggc gagatcggtc accagaaccg cgtcggcgac gctgttgaga atcgtgaagg
     9001 tgcgctcctg ttcgcgacgg atttccgccg tgcgctcggc cacgcgcgtt tccagcgacg
     9061 tggcgtacat ggcgttctcc acggccacgc cgagctggct ggcgagcgcg gtcagcacgg
     9121 ccagatcctc ttcgccgaac gcgtcggcgt ggtcgctctc gacgttcagc accccgacga
     9181 tcgacccctt cgccctgagc gggagcgcga ctttcgagag cgtgcgctgg acttcgggaa
     9241 cggggacgta acgcggatcg cggcgcacgt ccggcgccac gagcatctcc ccggtgacgg
     9301 ccacggcgcc gataatgccc acccccaccg gccttcgcat tccggccggc gtccagcctt
     9361 ccacatcgcc gccggcgccg gctcgccaca ccagctcgcc gccgtgaatc gtcaccacac
     9421 tcacgtggta gtaaccgaac gcgccgcgga tctcctcgac caccgtctgc gccaactgat
     9481 ccagatcgag gatggacgtg atatccaacc ctacgcgatg gaccagcgcc agttggcgcg
     9541 cgcggcgctg ctcggcctgg aacaggcgcg cgttctcgat cgcgatcacg gcgtggctgg
     9601 ccagcacctg cgcgcgctcg atatcggcag ccgagaaggg gtcaggccga acggaatcgc
     9661 cgacgaccag tgtgccgatg ggcgcatcgt cctggaccat gggcacgccc aacagcgact
     9721 tgaggcccat ccggtgaacg agctcgcgat tggcgcgcgg gtcggtttcg gcataattga
     9781 tatactcacc ccggcgctcg accagcacac gcgccccaag gatagacgca tcgccaagcc
     9841 cgacgtgcac gccgacgaag ccctcccgcg caggcccata gcccgcgaag ccaaccagat
     9901 catcgccttc ggccaaccat accagtccgc tgtccacgtt gaacgcgccc gcactctcac
     9961 ggcagatctg ctccagcacc tcgtcaagct ccagggaaga catgatcgcg aggctggtgc
    10021 ggcccagcgc cgaagcgaac cgttcgcgct cgagcgcctg ctcgtacaac cgcgcattcg
    10081 tgatagccgc cgcagcctgg ttcgcgatca cgccgagcaa gtccgcctgc tcccggcggt
    10141 agatgccggc ctgctcgtgg tccaccgata gagtgccgat cacccggtcg cgcgcaatga
    10201 gcggcgcgcc catccagccg tgcacccgct ccatgcctgg cagcacttcc ccgttgccga
    10261 gttgctgcac atcctccaca acgagcggct cgcgggcatc gatggtgcgg cccgagaggc
    10321 tggcgtcgcc gagcgccagc gtcctgccga taatctgcgc gggctgctga aagccacggc
    10381 cggcgataat ccgcaggtga cgcgacgcct cgacgagcgt caccgtagcg ctggtgtacg
    10441 ggatgacgcg cgcgatctga tcgaacaggc gctcgcacag ctcgtccaga ttgagcgtgc
    10501 tcgtcagcac tcccgaaatc tcgcgcagca cgtcggccgc gacgcggcgc ttctgctccc
    10561 cctcgaagag acgcgcgttt tcgagcgccg tcgcagcttg atcggccaac aaccccaaga
    10621 tgcggacttc gttgtcgacg acgatgtgtg gagcgtgaaa gagcacgttc atcacgccga
    10681 gcactttctc acgcaccttg agcggcaagc cgaccagggc ttgcgctccc tgctcggctg
    10741 tcgcacgcgt gatggccgcg aacgccggat cgttctgcgg atcgttgacc acgacagcct
    10801 ggccgctgtg agcaatgcgc atggtcatgc cttcgcggcg cggcgtaggc cacccgatct
    10861 cctcgaagcc aggcgccgtc gagaaggcca gcaactccac cgtatcggtt tccgcgtcga
    10921 agctgaacag gtgaaccacc gtcgcggcga ggaggcgcag ggcgccgtcg gcgatggcct
    10981 gcgagacgcg cctggcatcc agagactgga tcatctccag gctgacggcc tgcaaggtct
    11041 ccagctcggc tacgcgctgc cgcgcttcct ggtaaagctg cgcgttctca atcgccacgg
    11101 ccgcatggtt ggcaatcgtc tccgcgatct cggcgtgctc gcggcgatac ctggcggggg
    11161 ttcggctgtc gaaggtcagc acgccgatcg cgctcccttt gacgacgagc ggcgcgccca
    11221 gccaaccacg cacgtgatcg agggcctgcc ccttcgctgc gctcagggca ggctctgcga
    11281 ctcccgaagg ggggagtgcc gaagggccat gcgccgcgcc ccgctcaacg cctgcgcgtg
    11341 cgtcggcgca gatcgagacc cgccgcgtgc ggagcacctc ttcggccaag cccggcggcg
    11401 agtcatgcgc ctccgccggc atgcctcggc ttacggcgat acgtaactgc cccgagcgtt
    11461 cggcccgcgt cgccgatagc tcgcccggcg caatgaagac agccgccgaa tcgtacggaa
    11521 tcagctcggc ggcccgatcg agcagcgact cgagaatcgc ctgcggctcg agtgacgagc
    11581 tgatgaccgc cgccagttcg cgcaccgttt cggccacctt tcgacgctgg cgctcaacct
    11641 caaacaagcg agcgttgtca atcgcgctgg ctgccacgtt ggcaatcgtc tgacacacgt
    11701 gcgcctcatc cggtgtccag gagcgaaccc gcccggtctc ccaaatctcg acatagccga
    11761 tcagtcgctc gcgcacgacg agagggagat acagcgcgct gtgcacaaca cctgcgcgga
    11821 gaacctcgcg caacgccgcg tcgatcgcgt cgtgatcggc ggtggtttca taaaccgtcc
    11881 agcggcgctc cttcatcgcc gtgaccagga tcgaaagccc gcccaggtct tgctccacac
    11941 ccacacgcga ttggcgctca ctggcgctgg cgcgagcgcc cacgtactgc gccagtagac
    12001 gccctacgtt ggtctggaga tcccacacgt aaacgcgggc gctcgtcgcc tcgacggcgg
    12061 tcacaatcca ctgcgccagg cggccgaaca cctgatcgct gtcgagactg ctgcccaact
    12121 cctggctggc cgcgaacagc aaggacagat tgccggcgcg cgcctgcgtc tgctggtaaa
    12181 gctcggcgat cgccagagct gcgccgatct gactggccgc cgtctcgaga atgatcacgt
    12241 cgtctgcgct aaacgcattc gagcgatcgc tctggatgtc gagcacgccg acgatacgct
    12301 cttcaatgcg caagggcacg ccgatctcgg agcgtgcccc ggcgccggca gggttgacca
    12361 cgtagtacgg atcggtatcg acgtcgccgg tcacgtaagt ctgccgggtc gaggcggcct
    12421 ggccgacgat gccacacccg acgggcagat gcgccatcga ttgcgcttcg ggcgcgtgct
    12481 ggcgatcgtc gctgcgcgcg atcggcgcca gctcgttcag tgccgggtcg agccaccaca
    12541 ccgcgacgtg cttgtaacca aaccggcctg ccatctgctt gactgcctgc tgcacaatgg
    12601 tgtcccgatc cagcgcgacg accagctcac ggcctagatc gttgagcagc cgaaggcgct
    12661 cggagcgctc gtgctcgatc tcgaagcgcc gcgcgttatc gatcgccacc gcagcctgct
    12721 gcgcgatgag cgacgcgttg cgcgcgtctt cctccgtgta gaatcccgcc tggtggtgat
    12781 ggagcgcgag cagtccaatc actttgtcgc ggacgatcag cggcgcgccg atccacgacc
    12841 gagcgcttcc atcggggacg ccgtgcgagt ttccatccgc ggctcggaca tcggcgacga
    12901 cctgcgcctc gcgactcacg acggcctgcc aggccgggtt ccgatcgtcg cgcggaaagg
    12961 cgcggccgat cacgtttccc ggatcgacca gcccgcgcac ggcgagcact cgcagcgtgt
    13021 tggactccgc cagcatgatg caggatgagt tatggggcac gacgcggctg gcctgatcca
    13081 gaatggtcgc caacacagag tcaagctcca gattggaggc cacggccgcg ttgacttcgc
    13141 gcacggcctc cgccatccgc tggcggcgcc tcgccgcatc gatgagacgg ttgttctcga
    13201 tcgacagcgc aatctggcga cctgccacgg tgagccagtc gagcgtttcg tctccgagcc
    13261 ggcgcgcttc gtacgcatac agcgtcatgg cgccgaccgg cacgcgcccg gccaacccga
    13321 tccccacgac ggtctgcacg ccctcctgca tcagcctgtc gcggtaggga atgcgtgggt
    13381 cggtcggcac gtcggtcacg acgatcgcgc tgccggcggc gagcgcgcgc ccgccaaccc
    13441 cttcgccgat gcgcgtcgga gacgtcttca cctcttccca gaacgcgccg gacagaccgc
    13501 gccagacgta gggttgcagt tcgcccgtcg ccaaatccag cagcgagata ccgatcgcat
    13561 ccatgcccga cacgcgcagc acctggtaaa gcgcctgctc gagcgaagcg cgcagatcgc
    13621 cctgatcgac caccgcgctc aacgtggtca gcgacgtcag gttgtcgacg cgtcgcttca
    13681 gcccctcgcc tgcctgcacg tacaactgcg cgttctctat cgcgacagcc gcttgattgg
    13741 cgaatagatc gagcggcgtc aagtcgtccg gcgccgggcg cagcccgctc tgcgggcgat
    13801 cgagctgaat ggcgcccagc agcgcgcctc cgatttcgat cggcgcgacc agtatgtcgc
    13861 cggactgcca cgcctgtgcg ccgggatcgc cgcccgtcac gaggtagcag cgcccgatgc
    13921 ggtactcgct gcgtcgccag gtcttctcgt cgagaccccc cggccaggca gcgctctttg
    13981 gcgatacgcc gcgcacggct accggcgaca tggttgcgga tcgtgcttca gacaaccaga
    14041 cgatcacctg gccccacttc aggccctccc caatcgcgcc gcagatcgta tccaacagcc
    14101 tatcgagagg ctggcgcagt tgcatctgct cgcccactcg cacgagccgc gtgagctgct
    14161 gcaattgatc ggccagccgc gactgcgtca cttcaaatcg ctgcgatcgt tcgagcgccg
    14221 cttgaatctg gacggcgaag gcttcgataa tcggctgatc ctctggcacg agatgaccgt
    14281 gaggataacc gaccatgatc aatccatacg tctctccggc gagagacaag cgcaggccga
    14341 actgctcctc gtagttgagg agcttcgcca acgggtcggc caaccgcttg aggcgacgcg
    14401 gcagcaattc gcgtacgatc tggctcgcgt ccgagagcac cacaggcgaa tctctggaaa
    14461 gaatcgactg gagaaccggc gaggaatcca ccggggcatg ataggccatc gggtcaaatc
    14521 cgagcatgcg ccgggcctgc gagagaactc gccggccaag ctccgtgtga atcatcacga
    14581 cggcatgctc gtcatccaga tagcggaaga ccgacagaat caggccgatg gaacgcaact
    14641 gctcgcccgc cgcgcggagc acgtcctgcg gagtcagcgc gccctgcatg gccgccgacg
    14701 cgcggcttag cgcctgcagg agccgttcgg atcgctcctc acgctgaaga tctcgcagcc
    14761 taaccagaaa cacggagcta tcttcgagcg ccagcggcgt gacgcgaacc tccaccgggg
    14821 tggcgggttt attcagcacg gcgcgaacgg gatgtgagtc ggcccagtcg gagagcgccc
    14881 atggcggacg ttctgccagt agtcgcgcga acggcgcgcc gatcagcgcc tgcgccggcc
    14941 gatccagcag cttttccgcc gcatgattca catgcagaat gtcgcccgcg ctgccgcaca
    15001 ggagcaacgc ttctgcgaca tggtcgagca ggaggcgata aagcgcatcg gacaactgaa
    15061 gctgtttcaa ctgggcctgc gttcgtgccc gctccttcgc ctgctcgacc acggcggcga
    15121 gtgtcgagtg gagcagcgcc aggtcctgct cggtcttcgc gacgaagcca cacgcgccgc
    15181 gccggatcgc ttcggcggcg tggtcgtttc gcgaaagcac ggcgacaggc aggtcaggcg
    15241 cggaatgaag aaggtggtca agcccgtcga acggacgagg ccagccctcc aaatcgacca
    15301 gtgcaatatc gtaccgggca cccctgaggc gctgcggcac atcttgcagg ctaggcgcgt
    15361 cggtaatacg aaagcgcgtc gtgcgcccgc gcagcgctcg atgaatccgc cgccggtctg
    15421 cattgtcagg ttcaaccagc aggagccgaa taggggacgc catacactcg caattcccta
    15481 cctatgacgg gtggcacaaa cgagccccag tagatccaat tttgcccggc ctggattgcc
    15541 aaatccaggc ccaaatcggg gatttggcaa tcccctcagg gcattttcgg atctactgaa
    15601 cctggaaggg gaatgccgtc agccgacggt tagagtctca gttaagggga ttatagcccg
    15661 gatcggacgc ggggcaacct ttcaattcag ttaggattgc atttacacac cgacgagctt
    15721 gtagccgcgc ccacgcacgg tcagcaaata gcgtggattt gtcgggtccg tctcgagctt
    15781 ggcgcgcagg cggctgacca gcttctcgat gcgggcatca tcaacctcgt cgatgtagtc
    15841 ttgcccccag acgctttcga cgatctgata tttgtcgcac agcttatcca ggcgtcccca
    15901 caaaagcagc aagagacggt actccagatc ggtgagcgtc ggcgcctggc gcccatccac
    15961 cgtcacctcg ccggcatcca cgtcgaccca cacgccggac tgcgccgcct ggcgcgcgcg
    16021 tcgctggcgg cgcacgaagc ctgcaaagtg ctgtccgaac acgcgcggcg gctcgccgac
    16081 gagaacgcct ttctcgcaca gagcggcgag caaagccgga tcgatgctgg cgctcgagtc
    16141 gagcacgaga tcgatgagag cgtgccgctc gtcgtcactc aattggctcc acagcttgct
    16201 gcattcggcg cgggcaactt gatcgtcttc cagacgctgg ctcgccagcg ccagcgcctg
    16261 gccgcgcgca cggccgggcg cgccggtgac cagcgccagg acgacgcgcg tgacggcctg
    16321 gatcattccg gcgtgcccgc cggcctgctc gacgacgaaa tcgatctcgt gtggctggag
    16381 cgccgcgccc tccacggcag cccattcgga ggcgagcgcc cgcgcctcgc cgtcgctcaa
    16441 catgccgagc gtgcgctgct ggccgacgaa cagctcgaca aactcgctca cgtcgctgtc
    16501 gacgctgcgc gcactgagcg gcgccgtggt cgccacgacg tagctcagcc gcctgccgaa
    16561 tttgtcgctc agcgcgcgga ggttgagcag gacacggctg tcgagccggg cgaacggctc
    16621 gtcaaactcg tcgaatagga atacgatgtt gcgatcgaga ttgcggctga gcgcatccat
    16681 cgcgtcgttg aacgcgagcg gcacggcgaa ctcgctgggc ggctggacga tcatccgata
    16741 gagatcgtcc aactgcgcgg cgatcgaacc gggcgcgccg atgcgccgga gcgcgtccag
    16801 cgccgctcgc agcgtgacct cgtagaagcc ctgcgctgtg agcgtgagca tcaagttgca
    16861 gtcgacgtag atgaacacga actcgtcggc gcgctcgccc aacgcccacg ccagcacttc
    16921 tggcgagcag gccgtgcgca gcagctccga tttgcccaca ttgctcaggc cggcgatggc
    16981 cgacgagaag ccttctgcgg ctgtgcgaag aatgaattcg gcgtccgcag cgcggaaaat
    17041 gcgaggcgat gacatttttc agcaaaaggg gccaacgttg gcggggacgt tggcccggcg
    17101 ctcgttatgg gcgtctggtc gctacgtctg tgaagtgcgc agccggcgcg caatgaacag
    17161 taagacgacg agtccacccg ccagcgccaa gccagccaca tcgccgatgc cggtgcccgg
    17221 catggtgccg gtcccactgc ccggcgtgcc tgtcgcggag ggtgtgcgtc tcgcggcagc
    17281 cgtcgcagtg agcgccgcag gcggcgttgc aggcgactgg cgcgtggccg tggaggtcgc
    17341 tgttcgcgcc gccgccgcgg caccggcggg cgtcccggca gcgggcgacc ccgtcgcgat
    17401 cgcggtcgtg atcgctgtcg cagtggcgcg aggcgtggcc gtcggtgcac gcgtgtcggt
    17461 gggagcgggc gtgtcggtcg gagcgggcgt gtcggtcggc tccggggtga acgtcggctc
    17521 gacggtcggg ctgacttgcg ccaccgccag cgcggtcgcc gtctgatcgg ccacctgctg
    17581 cgcccgtccg ttccggttgc tcagcgcgat cacgatgacg atgatgccca acacgagcac
    17641 cacagccatg acgccgacgg ctatgatgaa cgtgcggtta gatgattcat cgccgttgcc
    17701 attggtggcg ttcatctgtt cagtggtcat gcggtctccc tccggggtca gcccgctcca
    17761 ttttagcgca agagttccag atttgcgaac ggtacaaacg ccgtcgaggc cgggctgaat
    17821 cggacgtctg cgccgtggac tctcgttccg atttcgatgc gcactgtcct cgtcggaatc
    17881 gccgtcaccg tgccgacctg tcccatatac ggaccgcgca ccacgcgcac gcgcgcgcct
    17941 accgtcaggg gcgcgccgac ggcaggcggc aacggcgggc gagtctcggc cggcagcgga
    18001 atgaggatct cggggcgaac gatgccccaa cgtgtacgtg tctcgccgtt aatcgacgcc
    18061 tcgcgcccat cctgcgcccg aatcagctca aagatgggag tcgccatccc tatccgcccc
    18121 acgccttcgg tgacgacgat cggaaacggc atctggcgcg ctgtctcgag caggtcgcct
    18181 tcaaggctgc cagcgatcat gccccgcaac tgaagctgct gcgcctgctc cagcgccgac
    18241 gcggtgatcc agccgccgcc gacgacaatg ctgccgtgcg cccggatgtc gatcgagctg
    18301 gctcgcagtg cctcgtccgg ggcttcactc accgcgcgca gaacgccgaa cgactcgccg
    18361 ccgatgccca gggcggcctg gatcaaggcg ccgggcgtct cgatgaccac gcccagatgg
    18421 cccaacatgt tgctgacgac gcctttgacg taggcgcgca gctcgaacag ccggctggcg
    18481 acctcgatcg ccacgcggcc atcgcgctcg tccacgtcga cgatcacccc atccacaggc
    18541 gatcgcaccg gccgaacccc caggcccatg cgcccggcaa tgacctcgcc ggctttgacc
    18601 acatcgtcct tgcctttctt gagataccga accgctctgg ccggcggtac gcgcagcaag
    18661 cgcgcgattt caaacgtcag gaactggccg cccaactcga cctgcgccac cacatccgag
    18721 gcctcgacgc gctggccagg tccgaccagg atattgccgg ggacaggcag catgcgatcg
    18781 cgccggatca gcgtcagcgg catcacgcgc gtttcggggg gatagtacgc cgtcgtctcg
    18841 ggcatctatg cacccacatc ccacagccag cttccgacac ggattcgcac ggattgcacg
    18901 gaaataaaga gcatctatgc acccacgtcc cacagccagc ttttgatctg gtcgcggcgg
    18961 cgcttcggat cgggcgacac gccgagcggg cgcccgcgcg cgtcgacgat caatcccaga
    19021 gcgctgcctg tgatctcggt cggcttaccg ccgcgtcccg ggccccgccc gatgtcgatc
    19081 ccgactcgcg gccggagctg catgactgct ttttggccgg gactgagcgg caatacggag
    19141 agcgatccgg ccttgatgtc gtcttcgatc gttccgccgc tctcgtagct gagtcttaca
    19201 ttcatcacga cctcgccggc gcgcgcgcgc atcgcaccga gcggcgcgac gaccgtcgcc
    19261 agatgcatga gagcaccctg ctccagcgcc tgcacggcga tcagcggctc ggccagggcc
    19321 gccgcaccga gcgcggcagc caggccgtac acgtcgagga cgagtgtcgt cacgccgact
    19381 ggctcgatcg cgtccagcat cagcaaagca gcctgcccgg gtcgcggcgc acgcgccagc
    19441 atgccgcctg cgccgatgat cggctcgaag aggggcacgt ggctgtcgga gaaacgcgga
    19501 atgctcgtgc cgtgcggcca ggcgccacgc gcggcacgca aggtcgcccg gataacctct
    19561 cgcgcaaggg cttgctcgat gcgcagctcg ctcgtgtctt gcgggatcgt gcctggacga
    19621 agctccttgt tggcggcgaa cgcccgaata tcgccttcgc tcacttcgaa atccagccag
    19681 cgcgcgacgg attcccagcc aatctcctct acgaagcgcg cgacgaacga agccccgacg
    19741 tcgctgcgca gcgtcgtgta gagctgcccg ccaaaggccg ccgcgaccgt cgtcgtcgat
    19801 ccaccgacgt cgacgcccag cacgccggtc ttcctgtttt ccgcgctcag gtactgtgct
    19861 acgtggccga acgcgcgcgc ggtaggcagc ataatcgagc gcttgctcca cgcggaaatc
    19921 gtggccagtc cggcgatgcg gcccatcttg tgcgtggtgt acatcgtttc cagctcgacc
    19981 tgcagggggc cgacattttc cacgtcgagg ccgggtcgca cgttgtcgat ctggagaaaa
    20041 tcggccgcat cgccgaccgt ctcggccacg cgcggcttca acctggcgtt tccggcgaac
    20101 aagatacgag gtctgctgcc ggccacttcc agaaagcgcg cggcgaactt ggcggcctct
    20161 accaactcaa gcaccggccg ttccgcgccg ccgtccgtgc cgccgctgat gcacacgaca
    20221 tccggacgcg catcgtagat gcgctgaatg cggtcgtcgg caatggagcg gcccgcgcgc
    20281 ctggaaccac ccgacagggc aatctcggcc gcgagcgtgg tataggtgcc catggcggcg
    20341 tggcgaaggc tctccaggct ccactcgccc gcgagtccgg ccagcacaac gctcaacggc
    20401 ttgccggcgc tggccgtcac gacgcaggca tccacgcctg agccgtccgg ctgctgcggg
    20461 gtcgtcaagt cgccgcgctc gttgagcagc cggcggcctg ccgccgaaga cagtttctcc
    20521 agcgcgtgtc gcgcgccaac ggccacgtcc gaccaggggg cctccgccgt cgtcaccgac
    20581 tccgcacggg cgatgaaccg ataagcgcct tcgacgcgtg cgatcagcgc ggctttcgtg
    20641 tacaccgtgc cgatgtcaat ggcgacgatc gaatcgagct ggctggagga tgcgtccatc
    20701 aggcggcctc acccaccggc cgggacgcgc gagaccagat cgcgcaccca ggcgaccagc
    20761 atactcgcgc cggagatgaa tacgagcgca aacgtcgcgc ccaatgcgac gtaaagaaaa
    20821 ccttgcccga tcagtcccat cacgcccagg ggcgcccgcc gggaggcagg gcgataggtg
    20881 aacgccagca gcgtcgtgac agtcgacgcg agtagaagca gaccgatgac gatgtccccc
    20941 gcttcgcgcg agcgaagcga gccggccgcg gtctcggccg cggcgctcgt ctgcggaaga
    21001 agtgtgccga acagcgcgcc gcccactgcc acacccacgc ccacgcccag catggtccct
    21061 cccgccacat tgccaagcca cgccgtgcgc gggaaaatct tggcaaggag gaacacgcag
    21121 cccagtagcc cgaatagccc gaacacgagc ggaccaatgc cgtcagcggg tcgggccagc
    21181 ggcaccaaca tgcgcggcgc caacacctgg ccgatcgcga cgccgacggc gtaggccgcg
    21241 cccacgccga cgaggaggtg catcgccagg cgataggcgg cgttgtcgcc gatcagatag
    21301 gacagcacgg caaacgtcaa aaccgcgccc acgacgatct ggatagtttc aggctgcatt
    21361 cccgctccct tcggtcgggg aaccttcgac ttcgctcagg gcaggcttcg cagcgcgtcc
    21421 gttccttcgg cttcgctcag gacaagcttt ttccggctaa agcctcgact cccccggcgc
    21481 ttcagcgtct cagcgagctt cagcttgcat cagggcgtct gcctcttccg gccgccaggg
    21541 acacgatcag accggccagg ataattgcgg cgaatgcgat ctgtgacaaa tagagagctt
    21601 ccgatctcac caggcggtcg aagcccggct ccccggcatc ttgatcggga tcgagtgagc
    21661 gccgataggc caggccgccc atcagtcctt gcacgaccgc gtccacctgc tcggattgaa
    21721 ggtagggctg aatctgcggc agagcacccg cgctcgcccc ggcgacgatc ggcgcctcgg
    21781 cgcccgagaa ggttgcctgt tcgacccacc aacgcatggc ctcgggcgag cccgcgagca
    21841 cgataatcaa gtcggcaggc acggacagga ttgcgctttg cgcgccgacc gcctgcccag
    21901 ggtgaaacga ggtctgcacg aaggcggcgt ccgggatgcc cttctcgccg aggctttcta
    21961 cgacacgcgc gccgagcgcc acgccgttcg gattgagact gacggcaatc gcctgcgcgc
    22021 cgcgctggcg gatgtggacg agaaacgcct cggcaatcgg atccatctca ccggcttggg
    22081 aagcgtcgta gtcgaacgac atgagcacgc gctgtccctg atcgagggaa tcgatctgcg
    22141 cggcggcgcc ggcgtacagc gcaggatcga cgctgccaag ctgaaagatg cccaactccg
    22201 ccatgaagtc atccaagttc ggcgcgaagg ccgccagtac cagggcgacg acaatcaccc
    22261 agcggacaat gtttgcgcgc aggcgtgcct gagctccggc cgtctccggc ttgacggcgg
    22321 tcggggcgag cgcgcccgtc gacaggagtc gttgaaagag ctcggcgcgc gcgatatcgg
    22381 ccggtggaac ctgcgcgtgc cgggcggccg gggctccctg cggcttgccg atgaatggcg
    22441 tggcaggcag aacgttttgg agacctgcca gcaatccctc ggtctcgatg acaccctctt
    22501 cggccgcacc tgtgggtgcc gcaaccggtc gcagagcttc aagccacgac ggtatcgctg
    22561 ctgcttcgag gtccggtgta agcgttcgcc cctgctcgtc ggcgaaggct ggggtggtca
    22621 ggcggtccgc cagttgcgcc tcgcccggag ggatcagctc ccgcgcttcg ccgggcggcg
    22681 atggcatctc gccgaagggc gtttcggatg gggcaagcgc cggcatcggg cctatctcac
    22741 gcagccagtc gggcaattca gagggcgcag gctcctcgcg ttgctcgggc gcttcgcgtg
    22801 ccggctcctg tacctgggcg agccaatccg gcgtggcggc caaatcggcc gtatcgatgt
    22861 cggctccggc gaacgccgac acatcgcccg gtggttccac cccgctgggc ggggcgccag
    22921 cagccgcagg cgcacctgct tcctccggcc cgatcgggcc tgcgcccaga tccttaagcc
    22981 agtctggtac ctcaccggcg ggttcttcgc cggtcggtga aggctcctcc agtgtcgtcg
    23041 acggcggcca gccgcgccag tcttcagcag gtggttcccg ttcctcggcg gggggttgcg
    23101 cctgcagatc ggccggccag ccatgccctt ctccagccag tcctgtctca gcaggttgcg
    23161 gctgctcgga gagtgctcct tgttccaggt gccaggtgtc aggtgcgggg gatggtggct
    23221 ccactggttc agaggggcgc tccgatatct ctggagcggc ggcctcaagc cccagctcag
    23281 ccagccaatc tggaatctca ccggcaaccg gcggctcgcc gaggcctggc tgaggctcct
    23341 gcgccgccgc cgtcagccaa tcgagcgacc cttcgactcc gctcagggca ggctcttcgg
    23401 cttccccttc gctccgctca gggcttcggc taagggtagg ctcgtctgca ggcgcagcgg
    23461 acggcggggt aagctccttg agccagtccg gcaggccagc ctcgccctcc ggggcctggg
    23521 tcgcctcttg ccgcatcgcg ctgccggatg caagatccag catccacgcc tccggctcct
    23581 cttcgacagg cgcaggggcc ggcttctcgg gtgaagacgg tgggggttct ccagccaggc
    23641 gatcgaacca gtcaagctgc tcgccctcag ccggccgcat ccactcaggt atttcctcgc
    23701 cggatggaaa ctcgggcgct gcctgagggg gaggcttcgg cgtcgcggcg cgtagacgat
    23761 gcagccattc cggcgtctcc tccggctcag cttcaggctc aagtcccggt gtcgtctccg
    23821 gttcgccggg aggcggctcg atttcatctg caggcttggt gggcagcgac aatccctttt
    23881 tcacgggggc gggcggcgcc ggcgcgcccg gcgccaccgg cggcgaagcc ggcccggccg
    23941 gcacgagccg cgcctggcat ttgtcgcagt agacgttggc cggcgggttc atgtggctgc
    24001 acatcgggca cacgatgccg gtcttcgacg gcagcttgtg cccgcagtcg ttgcagaagc
    24061 gtgagccatc ccggttggca gtgccgcaat taggacaata aatcatggct agtcgccttg
    24121 gggcttatct gccccgacga taatccgcag accgaaggcc agactaccca gcgccacgcc
    24181 gagcgccagg ccgcgtaagc tgccggtcgt aatcgagtcg acaacggcgc gaaacgaccc
    24241 gagtacgcca aggagtggaa cgacgggctg cgtgagcgcg acgacgatcg cggtcgctac
    24301 gaagagcacc atcccgaccg atcggcgcac ctgcagggcg cgaaagccgg ccaatgccag
    24361 cacgaacgcc aacaggattc ccaggccgga ctgaagcggg gcgatgaccc agtcgaacgc
    24421 actgacgaga aacccgggat cctgcatcgc gacggtcaag ggtacctgcc gttcggccat
    24481 gaccgatagg atgtgggcga tccatagccc aagcgtgaaa agtgcgccgc cgattaagac
    24541 gacgctgtac agcacgccct cgccgctgcg aatgcgccgg gcgtgaatgc cgatcaggtt
    24601 gccgaagccc agcaccagcg cgaacgctgc gaggttgacc gcccattcga ccagcacccc
    24661 gcgcacgttc aggagcgccg cggcagcggg gctatccgga tcgagcggca agtaactgat
    24721 caggatcagc caggcgaacg cgatggtcag tgcgacggga aatgcgcgct tgaagagcat
    24781 ggcacagtca agcaaacaac agcaatatcg accagatgac gatgacggcg gctacgatga
    24841 agcgcaccag gtcttgcgcc tgcaggcggg caatatggcc tgggcgttga tcgaggtagg
    24901 cgggggcggc gaacacctct tcgccgacga gcaggtgatt ggccgacgga aagagcacgg
    24961 cgagcgagcg cgcatccgtg gaacctgcga cttgcgtgag tccctgcttc acaccttcct
    25021 cgacgatcag gcccgcttct gggccgaacg ccccgaccat cacattcgaa accggctggg
    25081 cgtgcgccag gaaatccatc gcgccggccg catactggaa cggctgcatg ccgagcagtt
    25141 gcacgtcgcg tggatcgtag ccggcgaggt cgcctcgccg cgcgtaggga cgtcgcagcg
    25201 tatcttgagc caggatcatc gccgcgccat cggcgacggt caccgtgggc gctgcgccgg
    25261 tttcggcagc ctccggcgcc aggctgtcga cgatcgacgc gccggccagc gtgacgagcg
    25321 tatcgtcgcc gacgatgccg ccgacgccga tagaaatgtg cgccgggcgc ccggtctcga
    25381 tgccgcgccc cacctggccg cgcagcacgt cgaacgcgga tagagggcgc atctgcggca
    25441 cccgcgcgcg tctcgccgaa gtactgaaca ccagcatcgc gatcggcagc gacagcgcga
    25501 tgacgacgag agcgatctgc agcaagagtt gaataatcat gtgcgcgcct cttcgtccgg
    25561 ttcagccggc gccacctcgc ggctgtcgct tccctggccc cttccgggca gtgcaaacct
    25621 ctcggatagc cgatccaggg cctcggggga ctcaagcaga tcggccaatc cgctaatctc
    25681 ggccggcgaa gcgaaagtcg tcaggatcgg cgtgaataga tgcagagcct gcgctccaag
    25741 gaatgtaaag gggcggagca cctccaccgc cagcggcacg aaatcgccca ctccccaccg
    25801 ccggaggcgc gaggcccaca agaccacctg ttcttcgtcc cggtcggcat tcctgctgat
    25861 cataagtcgc ggctagtata gcatgctcag atatgcactg caagtaaggc attagtttgc
    25921 ggggccagac ttcccagccc gtggagcctg aacgcaaccg gcgcctggga tttgaaggct
    25981 ctgaagtgag gttggcacaa tcactgacct cacgatatac tgacacacgg atggcgcatt
    26041 ctgtccagat cctgcaacct gtgggaacag gattcggcgc agattggaat caccgacatg
    26101 agcaaggatg aaaaactcta cggtcacctg acaggccgca ggcgttgggc aatcgtgctt
    26161 ctcggcgcat tgtcaggact ggccatcgcc ttgattttcg cgtggtttgc ggggcgatcc
    26221 gcaacgatgg ttgagttggt gatcggggtc gtcgcgggcg ggttgctggg actgtcggcc
    26281 agcctgggtg agaagcggag acgcaagacg taaagcgtgt tgcgtgatcc gtgacagcat
    26341 ggcacacagc ctggttcgcc aactcacagc aagcgttgag gttggcactc caacacaacg
    26401 cggagagcga ggcgctactt ccacgcctcg ctctccaatc tccaatctct aatctc
//
LOCUS       DBNHXB010000129         5612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3716008, whole genome shotgun sequence.
ACCESSION   DBNHXB010000129 DBNHXB010000000
VERSION     DBNHXB010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3716008"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1667)
                     /gene="glyQ"
                     /locus_tag="AC162G_04980"
     CDS             complement(<1..1667)
                     /gene="glyQ"
                     /locus_tag="AC162G_04980"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIR5075201.1"
                     /translation="MSKPLSFQQVILKLQEFWASKGCVIWQPYNVQVGAGTMNPATFL
                     RVLGPEPWNVAYVEPSIRPDDGRYGENPNRMQMHTQLQVILKPDPGNPQELYLESLEA
                     IGIDRARHDIRFVEDNWEAPALGAWGLGWEVWLDGLEITQFTYFQQAGGVTLDPVSVE
                     LTYGLERIVMFLQDKHSVWDVDYDGALTYGDVFLRSEIEHNIYNYELGDVDRLRRMYD
                     LYEAEAKSALAHQPPLVIPAHDYVLKCSHTFNLLDARGAIGVTERAGYFARMREQARQ
                     VATAYVEQRQAMEYPWLGELEWRVRVGVGAKESRRASESASRRGGAARPISQRTDRPT
                     TFVLEIGTEELPAGDLSDALAQLKEAFPKFLKELKLDPLDVRDSLGTPRRLALMASIP
                     TRQPDEEVEARGPAMKVAYDAEGKPTKAAEGFARGQGVSVSALKPYSGDDRYVGVKKK
                     VAGRPAMDVLSENLPSLVASLKFGKTMRWNATNVTFSRPIRRLVALLGDQVVAFEYAG
                     VTSGRVTYGARPAGSPDLEIESADDYLATLQRAHIVASADERRATIR"
     gene            complement(1789..2577)
                     /gene="recO"
                     /locus_tag="AC162G_04985"
     CDS             complement(1789..2577)
                     /gene="recO"
                     /locus_tag="AC162G_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR5075202.1"
                     /translation="MPDRGRLYRTEAIVLRRSDAGEADRLLTLLTPDRGKLRVVAKGA
                     RKIASRKGGHVELFNHVGLLIARGRGEFDIVSQAETIEPYRALREDLHRSTYAHYAAE
                     LADRFAEEGTAQPELFDLLSDALRWFSTASDLALTARYFEVRLLTLAGFQPQVFHCVK
                     GGELLAEIADDETYGWSPHEGGALCTRHAAGRSDVSRLSLGALKVLRHAARSEYHAFA
                     ALTVREAVALEVERAMYRYLTYVLERKLQSVEFIHVLRREGVNG"
     gene            complement(2627..3991)
                     /locus_tag="AC162G_04990"
     CDS             complement(2627..3991)
                     /locus_tag="AC162G_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR5075203.1"
                     /translation="MEVGGIGSEFILLAILISLNAFFAASEMAMVSVPRLRMKQLMES
                     GNRTAGVVLRLADDSSRLLATVQIGVTLMGFLASATAAINLSGGLAQLIAQLPIPGIA
                     SAAQGIAVFSITVVLAAATLILGELVPKSVALAHSERIALLVARPIDLMARFFGPIVR
                     FLVWATNLIARPFGGKPRRGMPIVTQDEIKTLVDAGEEGGVLEVAEKKMIYSVFDIGE
                     MLAREVMVPRIDMVALEAGTPLLEATDVAIRHGHSRIPVYEETVDHIIGILYAKDLLK
                     VFRQHGRSGGDGVRLPSLTRPAYFVPETKKVDELLRELQQRRVHMAIVIDEYGGTAGL
                     VTIEDILEEIVGEIRDEYDESEEPMVKPLGRDEYEVDSRIPIDDVNELLAAELSDEES
                     DTLGGLIYNQLGHVPEPGEEIEVDGVSLKVLNVKDRRIGKVLIQRGAPPEPAGSQNGG
                     DGVD"
     gene            complement(4102..5442)
                     /gene="glmU"
                     /locus_tag="AC162G_04995"
     CDS             complement(4102..5442)
                     /gene="glmU"
                     /locus_tag="AC162G_04995"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR5075204.1"
                     /translation="MKLATIILAAGQGTRMKSELPKVLHPVAGRPMVSYVIDTAVALG
                     DERPTLVVGYRADQVRAAIGDRVEYVDQPEQLGTGHAVMQARGSLEGRSDVVLVLYGD
                     TPFVSTETLRAMLDDHVSSDAIITLLTFRPPDPLRYGRIVRSGSGRVVNIVEFKEATP
                     EQLTLREVNSGILCFKAGWLWSHLNQLQPRHGHGEYYLTDLVALAAAEVKTIATREAT
                     TEEVGGIDDRVRLAWAERLMRERIAERLMLSGVTIVDPSNSTISAEATIEPDTTIYPG
                     SHVEGATHIGRGCQIGPNTIVRDSVIGERCRIESSVVEGATLEEDVRVGPFAHLRSGA
                     YLERGVECGNFAEVKNSRLGAGTKQHHFSYLGDATLGRKVNVGAGAITCNYDGAKKHH
                     TDIGDGAFIGSDTMLVAPVRVGAGSKTGAGSVVTRDVPDGELVYGAPARAKPRD"
BASE COUNT          940 a         1893 c         1747 g         1032 t
ORIGIN      
        1 tgtctgatcg tcgcgcggcg ctcgtctgcg ctggcgacga tgtgcgcgcg ctggagcgtg
       61 gcgagatagt cgtcggccga ctcgatctcg aggtccggcg acccggccgg gcgtgcgccg
      121 tacgtgacgc ggccgctggt cacacccgcg tactcgaacg cgacgacctg atcgcccagc
      181 agcgcgacga gccggcggat tggccggctg aaggtcacgt tcgtggcatt ccagcgcatc
      241 gtcttgccga acttaaggga agcgacgaga gatggcaggt tctcggagag cacgtccatg
      301 gcaggccgtc cggcgacctt cttcttgaca cccacgtaac gatcatcccc gctatacggc
      361 ttgagcgcgc ttaccgatac gccctgcccc ctggcaaatc cctcggcggc cttggtcggc
      421 ttaccttcgg cgtcgtacgc gactttcatc gcaggccccc gcgcctccac ttcttcgtcg
      481 ggctggcgcg tcgggatgga tgccatcagc gccaatcgcc tcggcgtgcc aagcgaatcg
      541 cgcacatcta gcgggtcgag cttcagttct ttgaggaact tcgggaaggc ctctttgagt
      601 tgcgccagcg catcactcag atcgccggcc ggaagctcct ccgtgccgat ttccagcaca
      661 aaagtcgttg gccgatcggt cctctggctg atgggccgtg cagccccgcc tcgccggctc
      721 gctgactcgc tggctcgccg actctcctta gcgccaactc caactctaac tctccactct
      781 aactccccca gccacgggta ctccatcgct tgccgctgct cgacgtaagc cgtcgccacc
      841 tgccgcgcct gctcgcgcat gcgggcgaag tagcccgcgc gttccgtcac gccgatcgcg
      901 ccgcgcgcgt cgagcagatt gaaggtgtgc gagcacttga ggacgtagtc gtgcgcggga
      961 atgacgagcg gcggctggtg cgccagcgcg gacttcgcct cggcctcgta caagtcgtac
     1021 attcgcctca ggcgatcgac gtcgcccagc tcgtagttgt agatgttatg ctcaatctcc
     1081 gagcgaagga atacgtcgcc gtatgtcagc gcgccgtcgt agtcgacgtc ccacacgctg
     1141 tgcttgtctt gcaggaacat gacgatgcgt tcgaggccgt aagtgagttc gaccgagacc
     1201 gggtcgagcg tgacgccgcc cgcctgctgg aaatacgtga actgcgtgat ctccagcccg
     1261 tcgagccaca cttcccagcc gagaccccac gcgccgagcg ccggcgcctc ccagttgtct
     1321 tcgacgaagc ggatatcgtg ccgcgcgcga tcgatgccga tcgcctccag gctttcgaga
     1381 taaagctcct ggggattgcc ggggtccggc ttgaggatca cctggagctg cgtatgcatc
     1441 tgcatgcggt tgggattctc gccgtagcgg ccgtcgtcgg gccggatcga cggctcgacg
     1501 taggccacgt tccacggctc cgggccgagc acccgcagga aagtggccgg gttcatcgtc
     1561 cccgcgccga cctgcacgtt gtacggctgc cagatgacgc agcccttgct cgcccagaat
     1621 tcctgaagct tgagaatgac ttgctggaag gaaagaggtt tggacatgaa gctacttggc
     1681 tcgcttaggc tcgatgtcct gggggatgta tttgtcggtc atcatctcta ctccaaaaac
     1741 gaatcagcaa gtcagcgaat cagcgatatg tatccgttga ttcgttgctt atccgttgac
     1801 accctctcgc cgcagcacgt gaatgaactc caccgactgc agcttgcgct ccaaaacgta
     1861 cgtcaggtag cgatacatcg cgcgctcgac ctcgagcgcc accgcttcac gcacggtcaa
     1921 cgcagcaaac gcgtggtact cgctgcgcgc ggcgtgccgc agcactttga gcgcgccgag
     1981 tgacaggcgg ctgacgtcgc ttcggccggc ggcatgacgg gtgcacagcg cgccgccctc
     2041 gtgcggactc caaccgtacg tctcgtcgtc cgcgatttct gccagcagct caccgccctt
     2101 cacgcagtgg aacacctggg gctggaagcc ggccagtgtc aacagcctga cctcgaagta
     2161 gcgcgcagtc aacgccaggt cggatgcggt cgagaaccac cggagcgcat cggagagcag
     2221 gtcgaacagc tccggctgcg cggtgccttc ctcggcgaag cggtcggcca gctcggcggc
     2281 atagtgggcg tacgtcgagc ggtgcaggtc ttcgcgcagc gcgcggtacg gctcgatcgt
     2341 ctcggcctgc gacacaatgt cgaactcgcc ccggccgcgc gcgatcagca ggccgacgtg
     2401 attgaacaac tccacgtgcc cgcccttgcg gctcgcgatc ttgcgcgcgc ccttggccac
     2461 gacgcgcagc ttgccccgat cgggcgtgag cagcgtcagg agccgatcgg cttcgccggc
     2521 gtcggagcgg cgcaggacga tggcttcggt tcggtacagg cgtccacggt cgggcataaa
     2581 gtctcgctat tcggaagcag agcgggctcc cacgcccgcg aatcggctag tccactccat
     2641 cgccgccgtt ttgcgagccg gccggctcgg gcggcgcccc gcgctgaatg agaaccttgc
     2701 cgatgcgccg gtctttcacg ttcaaaactt tcaggctcac gccgtccact tcgatttctt
     2761 cgcccggctc gggcacgtgg ccgagctgat tgtagatcag cccgccgagc gtgtcacttt
     2821 cctcgtcgga cagctccgcg gcgagcagct cgttcacgtc gtcgatcggg atgcgcgaat
     2881 cgacctcgta ctcgtcgcgg ccgagcggct tgaccatcgg ttcttccgat tcgtcgtatt
     2941 cgtcccgaat ctcgccgacg atttcttcca gaatgtcctc gatcgtcacc aggccggccg
     3001 tgccgccgta ttcgtcgatg acgatagcca tgtgcacgcg gcgctgctgg agctctctca
     3061 acagctcgtc gaccttcttc gtctcgggca cgaaatacgc tggccgcgtc aggctgggca
     3121 gccgtacccc gtcgccgccc gatcggccgt gctgccgaaa caccttgagc aggtctttgg
     3181 cgtacaggat gccgatgatg tgatcgacgg tctcttcgta gaccgggatg cgcgaatggc
     3241 cgtgacggat cgcgacgtcg gtggcttcca ggagcggcgt gccggcttcc agcgcgacca
     3301 tgtcgatgcg cggcaccatc acttcgcgcg ccagcatctc gccgatgtcg aagaccgagt
     3361 agatcatctt cttctcggcc acctcgagca cgccgccttc ctcgccggcg tcgaccagcg
     3421 tcttgatttc gtcttgcgtg acgatgggca tgccccggcg cggcttgccg ccgaacgggc
     3481 gggcgatcag gttcgtggcc cacaccagga agcgcacgat cggcccgaag aatcgggcca
     3541 tcaggtcgat cggccgggcg acgagcaggg caatgcgctc gctgtgcgcc agcgcgacag
     3601 acttggggac aagctcgccc agaatcagcg tggcggcggc cagcacgacg gtgatgctga
     3661 agaccgcgat gccctgcgcc gccgatgcaa tgcccggaat gggcaactga gcgatcaatt
     3721 gcgccagccc ccctgacagg ttgatggcgg ccgtcgccga ggccaggaac cccatcagcg
     3781 tcacgccgat ctgcacggtt gccagaaggc gactcgagtc atccgccagg cgcagcacca
     3841 cgccggcggt ccggttgccc gactccatca actgcttcat gcgcagccgg ggcacgctca
     3901 ccatcgccat ctccgaagcg gcgaagaacg cgttcagcga gatgagaatg gcgagcagga
     3961 tgaattcact acctatccct ccaacctcca acttccaacc tccaacttcc aaccccaacc
     4021 tcagtccccg ctctcgctga gcctgtgacc cagcggctgg caccgggcaa aatacatccg
     4081 tgtaatccgt gtcatccgtg gttagtctct cggcttcgcc cgcgcgggcg ctccgtacac
     4141 cagctcgccg tctggcacgt cacgcgtcac caccgacccg gcgcccgtct tgctgcccgc
     4201 gccgacgcgc accggcgcga cgagcatcgt gtcgctgccg atgaacgcgc cgtcgccgat
     4261 atcggtgtga tgtttcttcg cgccgtcgta gttgcacgta atcgcgcccg cgccgacgtt
     4321 gacctttcgg ccgagcgtcg cgtcccccaa atacgagaaa tgatgctgct tggtgcctgc
     4381 gccgagccgg ctgtttttga cttcggcgaa gttgccgcac tcgacgccgc gctccaggta
     4441 agcgccgctg cgcaggtgcg cgaacggccc gacgcgcacg tcctcttcga gcgtagcgcc
     4501 ctccacgacg gaggattcga tgcggcagcg ttcgccaatg acactatcac gcacgatcgt
     4561 gttcggccca atctggcatc cgcgtccaat gtgagtcgcg ccctcgacgt gtgagcctgg
     4621 ataaatcgtg gtgtcaggct cgatcgtggc ctcggcgctg atagtggaat tcgacggatc
     4681 gacgatcgtg acccctgaca gcatcaggcg ctcggcgatg cgctctcgca tcaggcgctc
     4741 ggcccaggcc agccgcacgc gatcgtcgat gccgccgacc tcctccgtcg tggcctcgcg
     4801 cgtggcgatc gtcttgactt ccgccgccgc cagcgcgacc agatcggtca ggtagtattc
     4861 gccgtgaccg tgcctcggct ggagttgatt caggtgcgac cacagccagc cggccttgaa
     4921 gcacaaaatg ccggagttca cttcgcgcag agtgagctgc tccggtgtgg cctccttgaa
     4981 ctcgacgatg ttgacgacgc ggcccgaacc gctgcgcacg atgcgaccgt atcgaagggg
     5041 atcgggcggc cgaaaggtca gcagtgtgat gatggcgtca ctgctgacgt gatcgtcgag
     5101 catagcgcgc agcgtctccg tgctgacgaa gggcgtatcg ccgtacagca cgagcaccac
     5161 gtcgctgcgg ccttcgagcg agccgcgcgc ctgcatcacg gcgtggccgg tgccgagctg
     5221 ctcgggctga tccacgtatt cgacgcggtc cccgatggcc gccctgacct gatcggcgcg
     5281 gtagccgacg acgagcgtcg ggcgctcgtc gccgagcgcc acggcggtgt cgatcacgta
     5341 agacaccatc ggacgcccgg caaccggatg cagcaccttg ggcaactcgg acttcatgcg
     5401 ggttccctgg ccggctgcca ggataatggt cgccagtttc aacggatcct ccgtagatgc
     5461 gcggcgccct aacgcccggt cgtcaggcga gagacgcgcg atgatgacgg atatgacaat
     5521 gggaaggcca gcctgggcgg cgaagttggg tctgaatcag aatccatcat gaggggtgct
     5581 tctgcgtcac tgcccgtgcg ccagtcgttc ga
//
LOCUS       DBNHXB010000130         2679 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2038997, whole genome shotgun sequence.
ACCESSION   DBNHXB010000130 DBNHXB010000000
VERSION     DBNHXB010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2038997"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..443
                     /locus_tag="AC162G_05000"
     CDS             69..443
                     /locus_tag="AC162G_05000"
                     /inference="COORDINATES: protein motif:HMM:NF037170.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5075205.1"
                     /translation="MSVLKGLSVVSFDVTDWEGAKKFYGETLGLPVDFDAGKEAGWCE
                     FGEHGKTTLAISLWRQSSPPPRTGGATPVFAVDDCARAVGELRKRGVKCEDPVTIPGM
                     VSYANVFDPEGNRFQIAQSLAE"
     gene            686..2410
                     /locus_tag="AC162G_05005"
     CDS             686..2410
                     /locus_tag="AC162G_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HIR5075206.1"
                     /translation="MTVAKLILALSSILALILQPVPLITYHASRITQHASRIIPDRRF
                     GVVETYDDPASAAAIGAGWTRVRFPWSDLQPNNDGEWNNTFFTDDMLAAELAAGREVV
                     GLIVNAPHWALNDPAVLGVPRGLYLPESDPGNVWATFVRQIVSRYAGPLRVDRWIIWN
                     EPDIWDPAYPGRTWGGTVQEFFQLQRVAYNVAKQTNPNAQVHLSAFTYFWDTNYGRTP
                     FFSLLMDEIQKDPQSPSHNYYFDVASANLYFNVDNIYDLIVWHHGQMQARGFDKPIWL
                     IETNAPPSSDPSWPVANPAFPISLDEQAAFVPQAFAMALAAGAARVAVYKMIDTPGDR
                     AANPEPFGLVREDGSRRPAFTAYQLAANAFAGFTGASLDVRDERYTQVTVQRGDATTT
                     VLWARSPAPVQAQVSAHAPQAILVDAFGGRRAIAPRDGVYVVDLPACTQPQCAIGGAP
                     QILIEGAAVPNIPPVSSAPEQPTGQPIDRPTGTSTGTPTARPTPTPSPAGMPTRTPRP
                     TLTPTASALPTSTSAPTQTPDPVQVSAPTSVPASAAGPDALLIAIGVVFSVLTAAYAL
                     ARLGPSQD"
BASE COUNT          475 a          924 c          782 g          498 t
ORIGIN      
        1 aataaccccg gtcgagtcga ccacgtcgcg cgtggagttt cacgctgtca actgagggag
       61 gatgagccat gtccgtgctg aaaggactca gcgtcgtatc gttcgacgtc accgattggg
      121 aaggcgccaa gaagttctat ggcgaaacgc ttggcctgcc cgtggatttc gacgcgggca
      181 aggaggccgg ctggtgcgag ttcggtgagc acggcaagac cacgctcgcc atcagcctgt
      241 ggcgacaatc ctcgccgccg ccgcgaacag gcggcgccac gcccgtcttc gccgtcgacg
      301 actgtgccag ggccgtcggg gagcttcgca agcgcggcgt caagtgcgaa gacccggtca
      361 cgattccggg catggtctcc tacgccaacg tcttcgatcc ggagggcaat cgattccaga
      421 ttgcccagtc actggccgag tgacgcattt gcgctgcccg cacgccaaat tcccgaatga
      481 cgtagggagg cccgtggtga tcacgggcct ctcgcgttaa tctgcacgta acatccttcg
      541 ggtatcgtgg ctgcgattca atcggctgga ggaagaggga tgagaatgca tcctttttat
      601 tctctcattc tgtgctacaa tccccttcag acgcgacgca cttgcgcagt ccgcagtgcg
      661 ccgcgttgct gtgggagaag accggatgac cgtcgccaaa ctcatccttg ccctgtcgtc
      721 gatcctggcg ctgatcctcc aaccggtgcc actcatcact tatcacgcgt cacgcatcac
      781 gcaacacgca tcacgcatca tccccgatcg ccgcttcggc gtcgtcgaga cctacgacga
      841 tccggcttcc gctgcggcga tcggcgcggg ctggacgcgc gtccgcttcc cgtggtctga
      901 cctgcagcct aacaacgatg gggaatggaa caacaccttt ttcaccgacg acatgctcgc
      961 cgccgagctg gccgccgggc gcgaagtcgt cggcctgatc gtcaacgctc cacactgggc
     1021 gctcaacgat ccagccgtgc tcggcgtgcc gcgcggcctg tacctgcctg aaagcgatcc
     1081 gggcaacgtc tgggcgacgt tcgtccgcca gatcgtctcg cgctacgccg gcccgctccg
     1141 cgtcgatcgc tggatcatct ggaatgagcc ggacatctgg gacccggcct acccgggccg
     1201 cacctggggc gggacggtac aagaattctt ccagttgcag cgcgtcgcgt acaacgtcgc
     1261 caagcagacg aaccccaatg cccaggtcca tctcagcgcg ttcacctatt tctgggatac
     1321 gaattacggc cgcacgccgt tcttctcgct gttgatggac gaaatccaga aggacccgca
     1381 gtcgccctcg cacaactatt acttcgacgt cgcttccgcg aacctgtact tcaacgtcga
     1441 caacatttac gatctgatcg tctggcatca tggccagatg caggcgcgcg gcttcgataa
     1501 gccgatctgg ctgatcgaga cgaacgcgcc gccgtcgtcc gatccgagct ggccggtcgc
     1561 caacccggcc ttcccgatct cgctcgacga gcaggcagcg ttcgtgcccc aggccttcgc
     1621 gatggcgctc gccgcaggtg cggctcgcgt cgccgtgtac aagatgatcg atacgcccgg
     1681 cgatcgagcg gccaaccccg agccgttcgg attggtgcga gaggacggat cgcgccggcc
     1741 cgcattcacg gcctatcagc tcgccgctaa cgccttcgca gggttcaccg gcgcgtcgct
     1801 cgacgtgcga gacgagcgct acacgcaggt caccgttcag cgcggtgacg ccaccacgac
     1861 cgtgttgtgg gcgcgctcgc cggcgccggt gcaggcgcag gtctccgcgc acgcgccgca
     1921 agcgattctc gtcgacgcct tcggcggccg gcgtgcgatt gcgccccgtg acggcgtgta
     1981 cgtcgtcgac ctgccagcct gcactcagcc gcaatgtgcg atcggcggcg cgccgcagat
     2041 tcttatcgaa ggcgctgccg ttccaaatat cccacctgta tcctctgcac ccgagcaacc
     2101 gaccggccag ccaatcgatc gaccaaccgg aacatccacc ggcacgccca cggcgcgacc
     2161 gacgcccaca ccgtcgccgg cggggatgcc gacacgtact ccacggccaa cactcacgcc
     2221 cacggcttcc gccctgccga cttcgacttc tgcgccgacg caaactcctg atccggttca
     2281 ggtgtcggcc ccaacttcag tcccggcatc tgccgccggg ccggacgcgc tgctcatcgc
     2341 gatcggcgtc gtgttcagcg ttttgacggc cgcatacgcg ctcgcccgtc tcggcccatc
     2401 acaagactga ttctgacagt gcgagttcat tttgcacctt aaccgtaccg gagtacttgc
     2461 caatccgtgt gtcaggcagt ttgacgcgcc tgcgagcgcg tgctaacatg ccgtccgtct
     2521 atttccacac atacgaacac atgcatagac tcagcagatt aagaattgac agcggaggcg
     2581 gctcccatgc cgccgattcg cgggcgtggg agcccgctct gcttccgaat ttggatctac
     2641 tgagactcct catcctcgta ctcctgatcg ggctggcgg
//
LOCUS       DBNHXB010000131        22898 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_864695, whole genome shotgun sequence.
ACCESSION   DBNHXB010000131 DBNHXB010000000
VERSION     DBNHXB010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 22898)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22898)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22898
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_864695"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="AC162G_05010"
     CDS             <1..756
                     /locus_tag="AC162G_05010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5075207.1"
                     /translation="SRRYYNDQIARACEVADHILADSHATKSDLIGLLRVPAEKVTVV
                     WLAPDSRFRPQAGQAVRTQLDRLGVLAPYLLFVGTFEPRKNVTGLLNAYDILREGEKD
                     VPPLVLAGRRGWLFDETLAQVDRLGLRDHIVLLDTPSDDDLVALYSGAIALVLPSHYE
                     GFGLTALEAMACGAPVVISDRGSLPEIAGDAALVVDPDDVDALAETMRRVVGDADVRA
                     RLRERGLARAAEFGWERCARETLAVYGRVVDDA"
     gene            793..1992
                     /locus_tag="AC162G_05015"
     CDS             793..1992
                     /locus_tag="AC162G_05015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5075208.1"
                     /translation="MKLLFLTPQVPYPPHKGTTMRNYNLIAGLAERHTIDLLTFGDAT
                     IAAPPLSALCRRVRTLPAPARSMARRAITTLASPWPDMALRLWSPAFASSLEEWLRED
                     EYDVIQVEGIEMARYGLLARRLGLRARLVFDDHNAEWLLQRRTYEAERQLNGWNLGAV
                     YSLIQTWKLRRFERAACRAADRVVAVSDADAAAIHQLDPALPLTVVTNGVDTAHYAPG
                     AVEPLHFDFAALVFTGTMDFRPNVDAVLWFAERVLPRVRVAMPEARLIVVGQRPHPRL
                     DALRGRGDVLITGAVSDVRPYIAGASVYVAPLRMGGGTRLKVLEAMAIGAPIVSTSMG
                     VDGFDVQNGREVVIADEPDRFAAEVVNLAGDSRQRQALQDHGRRFVEASYDWRVIVPR
                     LEQVYND"
     gene            complement(2082..3473)
                     /locus_tag="AC162G_05020"
     CDS             complement(2082..3473)
                     /locus_tag="AC162G_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR5075209.1"
                     /translation="MIGGPFVLLLAPLIVAAATFFLRRFTVVSALLAVLVPLALAAWA
                     LWAPLDEPQAILGRVVVLGDGDRIAIALLFTIASAIFLGMWRSSPGWSFYPIMLMALA
                     ALAAALIIRPPLNEFYPSFVYSALFMGIAIALTVYPLQGGQPGVARGATRFITLTMLS
                     LPALLLADWVLAQSAQSPDSTSLVQGTILLLALGFMFLLAIFPFHTWVPAVSREAPPL
                     SAAFALTVFLGAALILLLDVLSENRVFTSDPRTLELVRGAALLMAGLGAVFAWAQSDF
                     GRLLGYGAVSDMGAILFAIGLSSSTGLAAAFFMIAMRALSVGLMSMGMTLARARLGDD
                     SFTTLTGSARRLPWTSIAMIAGGLSLAGLPPLAGFAGRWGLLQETSRLYPAATIVLLA
                     SSASVAVGVLRGLREMLRPPSEPVSVQQRERRSEAALIIAAVAVCLVAGLFPGILAPL
                     VREFVAAYSFAAR"
     gene            complement(3470..4153)
                     /locus_tag="AC162G_05025"
     CDS             complement(3470..4153)
                     /locus_tag="AC162G_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075210.1"
                     /translation="MTDLLSALSGLPSTTILTALVITVSLLVIVEDWRVLNLLLGVEY
                     LLIGALLIVAQIDGFPVVLAIVKSLVGVMIVPALYISARRARWGKSASDADEDGEAPA
                     RRFAWLRAPGLALHTIVAVLGAAVALSLALRSPVQFITPQSAARDLTIAVFVLFAQGT
                     LNVALSENPLKVGLGLLTVIAGFELLYTPVEPTLLIVALLGVVNLLIALAFSYLIAAW
                     ATGTRGAAP"
     gene            complement(4156..5787)
                     /locus_tag="AC162G_05030"
     CDS             complement(4156..5787)
                     /locus_tag="AC162G_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075211.1"
                     /translation="MSILPLVPILILLGGVLALLLCRAARFAHYDAVASVAALLALVA
                     IMLLAVPDLPVRETLISSWQPTSVFGTPVALRVDRVDWMIGLVATLACGATALAGIAY
                     PGRRRFGPRALALGMTAGLVAAAFSANVLTLALAWGLFDAFFVIAALMQGDESAAGRR
                     AAFAVGFNIAATLCLWIAALAINQDNESQYWHLTQLPEGARQFLALAAVLRLGLYPFS
                     QWLPAEREDVPGRVALLYVLPPLAGLHLLIRMASLDALPQEPAAIWFAALSVLAGGAL
                     AWWRSASQDALPSLSLSTLGIVVLSGIAGRTPTAPQAILSAGAVSWALAIVTLSLNHG
                     FHRRALWWSLGQALAVATLVGIPASAGFAVRTSLASAIAWGGNVLLLVCVVAGETLAF
                     ATMARLLTTPNPSRIPRSWRDVASLASAITLVALPAFLLPAIARRVVPAITPPAFAVV
                     LPDMGIPGSLLFILPLALAVTLEWLRRERRISMHVNPTRLLNLDWLYHLSFRLANVVA
                     SALNRLSAVIEGEGAVVWALLILFAAYVVLTGAIR"
     gene            6024..9317
                     /locus_tag="AC162G_05035"
     CDS             6024..9317
                     /locus_tag="AC162G_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5075212.1"
                     /translation="MSIQLPDGSILSFSLPQRFDSAVIVATYVLSAVLVFVISLPSFR
                     GLRRGQWALLACLAVISVPLNAILLVPASALASSTLAPLLSKLAPAPLLGWFCMLLAG
                     AWLGAGPAMLVGFTGGLGRWLLAGGRFTQPAEMALIAAAAAFFMQQNYRGRLASAVRK
                     PLVAGLIAGLLAAFISVFAVFASMPGEAPTAADYTLSTVGYATLLALGEGLINSLLVQ
                     LACLAFPSMRRMVSAARAPFYARSLSRRLWTSILPITFVTITVQVVVVGLTALNVAYR
                     QAETQLSRYTQATADLLDLFFLDGEAILFQFAQDGRLQSDDVRARQAYLESALRSIPF
                     FDELLLVDANGQVLAAARLPAVGDESPAQVTPQEESRLERTLQTGAPQRTSVYDDGQG
                     RPMMSYIQPSTSVVPPGGAVVGRTRLSTNPRIGAIRRTLPNTLDAGLGYVVDDNSLIT
                     IHPDPDAERRQWLFDRSQAAIGDSGSAYVDRYADGSRRVIVVREIANAAWQVATEMPY
                     SNIVRAAGEISVPLIGMFAVIMIGAFVLIPLISRRITQPLVHLTQAAGRIAQGELDRP
                     VTEAGEDEVGQLGVAFEGMRQSLKGRLEDLSLLLRVSRSVADSLDLERGIPPILEAAM
                     QVTSSQGQPPAARTARLLVFDEDGKPLRALTCGEGPAQLTPLDEALATITVRDERPLI
                     IENVARSRGALDPKLAGPGVRAIVTLPLWRSNRALGVMWLGYPEARRFSDSEVGLLVT
                     LAGQAAVFLENVGLYDAAEGGRKRLQAVLSSTIDAVLVTDSQNRILLCNPAAEAAFGL
                     SSGDATGRPAGEVITDPIVVDLLTERGDPVVRTAEVLMPDGRTLYGSASSILSGDNIR
                     IGRVAVLRDITHLKELDAMKTEFVATVSHDLRSPLTYMRGYVTMIPMVGQVETKQQDY
                     LDKITRGIEQMTTLIDDLLDLGRIEAGIGLVREPTRLADIAREAVESMQPQALARGLN
                     LRLVESVPGALRGDRQLLKQAISNLIDNAVKYTPAGGSVEVAVDERDSWAIVRVTDNG
                     IGIAAVDQPRLFEKFYRVKRRDTIDIRGTGLGLAIVKSIAEWHHGRVWVESHLGKGAT
                     FYIALPTEG"
     gene            9492..9779
                     /gene="rpsF"
                     /locus_tag="AC162G_05040"
     CDS             9492..9779
                     /gene="rpsF"
                     /locus_tag="AC162G_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR5075213.1"
                     /translation="MRPYELMFIVRPEVEGGDLNVVVDRVKSLVTGGGGQVTEVNAWG
                     RRQLAYPIHKIMEGQYFFVRAQLPAHALGGLERDLRLTEQIVRYLIVRADE"
     gene            9813..10211
                     /locus_tag="AC162G_05045"
     CDS             9813..10211
                     /locus_tag="AC162G_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR5075214.1"
                     /translation="MTRGLNKVMIIGQLGRDPEMRYTPSGRPVTSFSLASNRTWITSE
                     GERREETEWFNVVTWGGLAEICKQHLHKGQTIYVEGRLQTRGWEDQEGKKRFRTEIVA
                     SEMIMLGDRRGESADESIGEGVPADEDFPF"
     gene            10267..10611
                     /gene="rpsR"
                     /locus_tag="AC162G_05050"
     CDS             10267..10611
                     /gene="rpsR"
                     /locus_tag="AC162G_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR5075215.1"
                     /translation="MVEELRSAEPGNSASEERSGAAPGARGGSGPRRSRGGGGKRFQG
                     KPKVCLFCVEKGQHIDYKQADLLRRFMNESGKIRPRRQTGTCARHQRLLAVAIKRARH
                     LALIPFTTRPAR"
     gene            10658..11845
                     /locus_tag="AC162G_05055"
     CDS             10658..11845
                     /locus_tag="AC162G_05055"
                     /inference="COORDINATES: protein motif:HMM:NF025009.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HIR5075216.1"
                     /translation="MSKRPQASPLPKELSRKHISRAQRDAQRTRILLFAVGGAVVVAI
                     LLIAFGFVRETFITPNEPVAIVGNEQILTRQFQQRVRLNRYQLLQQYSLLQSFGSNDS
                     AAQVLQQLNDSISLGSQAINALVNEAIYRQAAPSLGVAVTQDEVDKLIEEGFGYYRNP
                     PTPAPTSTPRPTPTVPPDATPTPNATATATATPRPTSTPITAEGFQSLYQARLSELAG
                     FGFNEADFRRLFEMQLISERVQDIIVSDVPTMTEQATFQFIRALSQSDIEAVQQAIAE
                     EGFDAVYAQALSQTLPISNVLGSELPFLPKEDLIESPAFGAAVADAVFTTPISDMLAI
                     VTDPAGVYFYVGRVPAREVRELAPDALERRQAAAIEDWLTRQREQLNAQVLTWEDRVP
                     NDP"
     gene            complement(11932..12867)
                     /locus_tag="AC162G_05060"
     CDS             complement(11932..12867)
                     /locus_tag="AC162G_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR5075217.1"
                     /translation="MKTLQQFLGGLVSGAVVIALIASGLVLTAGDLRIASLTSVPSPT
                     ASAAPPISGPSALPDASAALTTSPTVVWTPTLVPTSTCPRPPGWIDHLVAEGEDLPGI
                     AARYGIDPVVLQVNNCLVAAFVTPNMILFVPAPPTLTPTVAVIATVVIRPTVCGPPLG
                     WTIYRVRPGDTLSSIARATGSTVQALMLANCLPSDKIRVGQILYVPRLPIPTVAIPFT
                     PTPTPTPSPSPTGTPTPTPVETLVTPEPTTPGAPSPSPPPTETPPAQSPTPTPTFDDP
                     PATDTPSPTVPTLTTEPPPTTVPPPTNTPGPNVTP"
     gene            complement(12933..15686)
                     /locus_tag="AC162G_05065"
     CDS             complement(12933..15686)
                     /locus_tag="AC162G_05065"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5075218.1"
                     /translation="MISMTQIVERLTQPPGSLVFHLVTLFSLTFMLYAAVGQIRAHGS
                     ANPLPRLHWAAGGMLLGQGLLLLAVVLENSDIVPPRSLVPPLDRAIDVIGIGLLALAF
                     VPLVTDDTRLGLIFAGGSAAAALIAYVAVAPAWYAASSQATMVYSEQDRGWQAWSLAL
                     TAVAAVLTLIRRRGQWAFVVIAFVILAGGHFLQAVAPEASTDIAGWVRFAQLAAYPLL
                     AAVVYREYVESRHEAIPAPAGAGLAPARSASWASDVWAVVDTIRQVSTSADLPLTYQK
                     ICAAVAASLRADLAALGLPGPAANLVELVAIHHPGAAPTPGAVFALEDHPAVRRAIDR
                     QRAVIIGPQHDAPDVSGLFGLMGSFVSGPLLIQPLVSHHTVLGVLLIGNPGSGRAISA
                     SDAQQARTYGELLSATLYSRQQIDTLQKRASEISETLHQQSIDLNAQRAAVAGAAQQA
                     QAEIEQLKAALLVAEQRAGQADRRAQELAALVEVQEAEKRALPPSPSSVWEDEARRLT
                     DERNTLEAQLRDAREEAAQLAALQQALETQLKHAQQQITRLRDDAERQAVKALATAAP
                     AEGGEPGVLVSDTLGRVTLVTDQAQRLIGKPRGALIGQPIHGVLSDRRWREALDLLTR
                     APNPTAAGEPPFHIDVSAAGKPLHAELTPFQDGSGETYNGIVVTLRGAAGPDGSPREE
                     VIASIAQELRTPMTSIAGYTDLLLGESVGILGAMQRQFLQRVKANVERMGGLLNDLIG
                     VAAIDAGRIKLEPEPIDAVEVIEEAIMSSSAQFRERGITIQLDLDEDLPQIQADRDNM
                     HQILSHLLSNASAVTPPGGEVMVGAHMNPEAPDFVLICVTDCGGGIDPLDRQRVFNRM
                     YRADNPLIAGLGETGVGMSIARALVEAHGGRIWVDSDMGKGSTFTFIIPVGGSTGGNG
                     AGA"
     gene            complement(15795..16517)
                     /locus_tag="AC162G_05070"
     CDS             complement(15795..16517)
                     /locus_tag="AC162G_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR5075219.1"
                     /translation="MQLYFIRHSQSANNALYDRTGSWSGRSHDPELTDIGIRQAEYLA
                     RFLRQANPGAVANGWDPHNVSGFGITHVYTSLMVRAVQTGSIVAEALGLPLMAWEDIH
                     ENGGLYLDGEETEEKIGQAGLNRAEFAARFPNLVLPNRLGEAGWWNRPPEDEEQWPIR
                     ARRFLSDLTVRHGSTNDRVAVISHGGFYRQFLLALLNAPAADGYWFLLNNAGITRIDF
                     DEGNVGLVYANRVDFLPRDLIT"
     gene            complement(16566..18083)
                     /locus_tag="AC162G_05075"
     CDS             complement(16566..18083)
                     /locus_tag="AC162G_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HIR5075220.1"
                     /translation="MEAKLRELKMRLMEISDIRSANALLGWDQETYMPPGGAEARGRQ
                     RATLGRIAHEKFIDPAIGKLLGDLRPFEEGLPYDSDDASLIRVTRREYERSVKVPPEF
                     VAELEEHAAASYQAWTEARPANDFRRMQPLLEKTVDLSRQMAGYFPGYEHIADPLIDE
                     ADYGMKASTLRALFADLRAQLVPLVEAIAAQPVADDSCLRHRVPEDRQLAFGLDVIKR
                     YGYDFTRGRQDKTHHPFMTKFSLGDVRITTRFKENDLTDALFSTMHESGHAMYEQGIH
                     MAYEGTPLAHGTSAGVHESQSRLWENMVGRSRNFWTFFYPKLQETFPEQLRAVTPDTF
                     YRAINRVQPSLIRTDADEVTYNLHVMIRFDLELQMLEGTLAVRDLPDAWHARYQADLG
                     LHAPDDRDGVLQDVHWFGSVVGGQFQGYTLGNILSAQFYKAALAARPEIPGQIAQGEF
                     GALHGWLTDNVYRHGSKFTASELVERATGKSMTIEPYLEYLRAKYGELYPLAAHT"
     gene            complement(18283..18615)
                     /locus_tag="AC162G_05080"
     CDS             complement(18283..18615)
                     /locus_tag="AC162G_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075221.1"
                     /translation="MQKHFTSSEPPSFKVIAAFLDQHGHLPASPEDIAQQIRAARAQR
                     SKRRSRSQDGVIGRSPSRESDVFTPLIAHDPRPYTEDFEKCPHGILKFKKCAICDPEG
                     FRLEHGWD"
     gene            complement(18850..21297)
                     /locus_tag="AC162G_05085"
     CDS             complement(18850..21297)
                     /locus_tag="AC162G_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR5075222.1"
                     /translation="MPGAQRRLSVLTAIGLTFAAAACATSPPSDTSPIAPPLSTAVPV
                     FTTPDADPTGAPTETPTPSPTATPTPTPLPAVRLGDAARHMRNGDFESAIAEYGAILD
                     DGGAASEVEAALFGAGVASLRAGDLVAAENMLGQFIDRYPDSALLGDAWFLLGDARHA
                     SGNFTGAVDAYREYLDLRGSVIGSYVQERIGDALNQAGDTEAAVVAYREAIAAAPNNP
                     VAARQREKLALVYRLRGDYDDAIAQYRAILGFAQIDAYRAGVLLQLGQTLIDSGDAAG
                     YDVFADLVSTYPKTDSAYQALVALVGNGIPVDFFQRGLVDYYARQYDAAVAALGEFID
                     SVEDHADAHYYMAMSHRAAGNTRAAIQQFDALIEDHPQSPLWAQAWIDKAIAQSQGDD
                     LEGAAATLTKFAEDTPRDALAPNALQRAGILLERAGEFDRAAEVFRSLQESYPSHTLA
                     PDALFGAGVNAYRAGRAEPAVEAWRVLSDTYPTADRYPAALLWQGKLAQHGGDARGRT
                     LLDLAARAKPYGYYGIRAAELRDDRPTLQPVPFDLEFDEAAERAEAEAWLAEWTGRED
                     AAGIGALPESILEDGRFRRGDELWRLGWVDEARSEFDDLRAAHNDDPVALYALSLYWR
                     DIGLYRSSLIAAARLIAISPAKTPDNAPAFVARLSYPTYYADLVVPEAEQHGLDPLLV
                     FALIRQESLFEGIAVSRAFANGLMQIIPSTGEFIASRLGWPDYSTADLYKPTVNVTFG
                     TWYLANVRDGFDGDVYAALAGYNGGPGNAQRWRDAAKGDPDLFLETITLDETRAYLLR
                     VREHLAIYQKLYGVSDP"
     gene            21486..22565
                     /gene="mtnA"
                     /locus_tag="AC162G_05090"
     CDS             21486..22565
                     /gene="mtnA"
                     /locus_tag="AC162G_05090"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR5075223.1"
                     /translation="MSESIERTIEWHESPGGAGGRGVVKMIDQRLLPHEVVVRSYTDY
                     RDVAEAIRTMVIRGAPAIGAAAAFGMALAALHSQAESRLDLLRDLEIAANVLRESRPT
                     AVNLFWAIERMLRAAVDESLDSTADVRERLIREAQQLADEDVDINRRMAEHGATLIRD
                     GDTILHHCNTGALATVDWGTALGAIRMAHLQGKRVHVLVDETRPRLQGARLTAWELMR
                     WGIPMTLIADNAAGHYMRTGKVDITLVGSDRTAANGDVANKIGTYKLAVLAKENGIPF
                     YPVVPTSTIDLNLKSGDEIPIEERDEREVTEIDGVRIAPVGVKVGNPAFDVTPHRYVT
                     GIVTENGIAYPPFELSLRKMVERKA"
     gene            22635..>22898
                     /locus_tag="AC162G_05095"
     CDS             22635..>22898
                     /locus_tag="AC162G_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR5075224.1"
                     /translation="MSENLPVVILCGGASTRMGGSGLDDRPKPLIHIGERPVLWHIMK
                     IYAAFGHTDFVLALGYRGEMIKRYFLDYEPMSRDFTVALGDARR"
BASE COUNT         4160 a         7135 c         7589 g         4014 t
ORIGIN      
        1 agccggcgat actacaacga tcagatcgcg cgcgcctgcg aggtggccga tcacatcctg
       61 gccgactcgc acgcgacgaa atccgatctg atcggcctgc tgcgcgtgcc tgccgagaaa
      121 gtgaccgtcg tctggctggc gcccgattcg cgcttccggc cgcaggcggg gcaagctgtc
      181 cgaacgcagc tcgatcgcct gggtgtgctg gcgccgtact tactgttcgt cggcacgttc
      241 gagccgcgca agaacgtaac aggcctgctg aatgcctatg acatcctgcg cgagggtgaa
      301 aaagacgtgc cgccgctcgt gctggccggt cgtcgtggct ggctatttga cgagacgctg
      361 gcgcaggtcg atcggctagg cttgcgcgat cacatcgtct tgctggatac gccgtccgac
      421 gacgacctgg tcgccttgta cagcggcgcg atagccctgg tcttgccatc gcattacgaa
      481 gggttcggcc tgaccgctct cgaagcgatg gcttgtggcg cgccggtcgt gatcagcgat
      541 cgcggctcgc tgccggagat cgcgggcgac gctgcactcg tcgtcgatcc cgacgacgtg
      601 gatgcgctcg ccgagaccat gcggcgggtc gtcggtgatg ccgacgttcg tgcgcgcctg
      661 cgcgagcggg gcctcgcccg cgccgccgag ttcggctggg aacgctgcgc gcgcgagacg
      721 ctggcggtct atggcagagt ggtggacgat gcgtgataac atgagagcgt gacgacgtga
      781 tggtgacata acgtgaagct gctgtttctg acaccgcagg tgccgtaccc tcctcacaag
      841 ggcacgacga tgcgtaacta caacctgatc gccggcctgg cggagcggca tacaatcgat
      901 ctgctcactt ttggagacgc gacgattgct gcgccgccct tgagcgcctt gtgccggcgt
      961 gtgcgcacgc tgccagctcc ggcgcgctcg atggctcgcc gcgctatcac gacgctggcc
     1021 tcgccgtggc cggacatggc gttgcgcctt tggtcgcccg ctttcgccag cagcctggag
     1081 gagtggctgc gagaggacga gtacgacgtc attcaagtag aaggcatcga gatggcgcgc
     1141 tacggcttgc tcgcacggcg actggggctg cgcgcgcggc tcgtgttcga cgaccacaac
     1201 gcggagtggc tgttacagag gcgcacgtac gaagccgagc gacagctcaa cggatggaat
     1261 ctcggcgcgg tgtactcgct gattcagacc tggaaactgc gacgcttcga gcgcgccgcg
     1321 tgccgcgcgg ccgatcgggt ggtcgccgta tcggatgcgg acgcggcggc catccaccag
     1381 ctcgatccgg cgctgccact caccgtcgtg acgaacggcg tggacacggc gcattacgct
     1441 cccggcgccg tcgagccgct tcatttcgat tttgccgccc tcgtgttcac cggaacgatg
     1501 gactttcgcc cgaacgtcga cgcggtgttg tggttcgccg agcgcgtgct gccccgcgtg
     1561 cgggtggcga tgccggaggc gcggcttatc gtggtcgggc agcgaccgca cccgcgcctg
     1621 gatgcgctgc gcggacgcgg cgacgtgctc atcaccggcg cggtatccga cgtgcgaccg
     1681 tatatcgcgg gcgcgtcggt ctacgtcgcg ccgctgcgca tgggtggtgg cacgcgattg
     1741 aaggtgctgg aagccatggc gatcggcgcg ccgatcgtca gcacgtcgat gggtgtggat
     1801 ggcttcgatg tgcagaacgg gcgcgaggtc gtgatcgccg atgagccgga tcggtttgct
     1861 gcggaagtgg tgaatctggc cggcgattct cgccagcgcc aggccctgca agatcatggc
     1921 cggcgcttcg ttgaggcgag ttacgactgg cgagtgatcg tgccgagact ggagcaggtg
     1981 tacaacgact gaagccgtta ctaccatctc gagggttggg acggcagttg cactgccgtc
     2041 cggagccgcc gcagtccaac tgcggcggga actgcagaag gtcagcgcgc ggcgaacgaa
     2101 tacgctgcta caaactcgcg cacgagcggc gcgagaatgc ccggaaacag gcccgcgacc
     2161 agacagacgg ccactgccgc gatgatgaga gccgcctcgc tccggcgctc gcgctgctga
     2221 acactgaccg gctcggatgg tgggcgaagc atctcgcgca agccccgtaa aacgccgacc
     2281 gccacgctcg cgcttgaggc gagcaagacg atagtcgcgg caggatacag gcggctggtt
     2341 tcctgaagga gtccccagcg tcccgcgaat ccggcgagcg gtggaagccc ggccagcgat
     2401 agaccgccgg caatcatggc tatcgacgtc cacggcagcc gccgtgcaga gccggtgagc
     2461 gtcgtaaagg agtcatctcc caggcgcgcg cgcgccagcg tcatgcccat cgacatcagc
     2521 ccgacgctga gcgcgcgcat ggcgatcatg aaaaacgccg ccgccaggcc ggtgctcgac
     2581 gacagcccga ttgcgaataa gattgcgccc atgtcgctga ccgcgccgta gccaagcagg
     2641 cggccgaaat cggactgcgc ccaggcaaag acggccccca ggccggccat caagagcgcg
     2701 gcgccgcgca caagctcaag cgtgcgcgga tcgctcgtga acacgcgatt ctcgctcagc
     2761 acgtccagga ggaggataag ggcagctcct agaaacacgg tcagcgcaaa tgcggcggaa
     2821 agcggcggcg cttcgcgcga taccgccggc acccaagtgt ggaagggaaa tatcgccagc
     2881 aggaacatga agccgagcgc caatagaaga atcgtgcctt gcacgaggct cgtcgagtcc
     2941 gggctctggg ccgattgcgc cagcacccag tcggccagta gcagcgcggg tagcgatagc
     3001 atggtcagcg tgatgaagcg cgttgcgccg cgcgcgacgc ccggctgacc gccttgcaat
     3061 gggtataccg tgagtgcgat ggcgataccc atgaacagcg cggagtagac gaacgaggga
     3121 tagaattcgt tgagcggcgg ccgaatgatg agcgcggctg ccagcgcggc aagcgccatc
     3181 agcatgattg gatagaacga ccagcccggc gatgagcgcc acatgccgag gaagatggcg
     3241 gacgcgatgg taaagaggag cgcgattgct atccgatcgc cgtcgcccag cacgacgacg
     3301 cggccgagga tggcttgtgg ctcgtccagc ggcgcccata gcgcccatgc ggccagcgcg
     3361 agaggcacga gcactgccag gagcgcagag accactgtga accggcgcag gaagaaagtc
     3421 gctgcggcga cgatcagagg tgcgagcagg aggacgaacg gcccgccgat catggggccg
     3481 ctccacgcgt acccgtggcc caggcggcaa tcaggtatga aaacgccagc gcgatgagca
     3541 agttgaccac ccccagaagc gcgacgatca gcaaagttgg ctccacaggc gtgtacaaca
     3601 gctcgaaccc ggcgatgacg gtgagcagtc ccaggccgac cttgagcgga ttttcgctca
     3661 acgccacgtt cagcgtgcct tgggcgaaca gcacaaatac ggcaatcgta aggtcgcgcg
     3721 ccgccgactg gggggtgatg aactgaaccg ggctgcggag cgccaggctc agcgcgacgg
     3781 cggcgccgag caccgcgacg atagtgtgca gcgccagacc cggcgcgcga agccaggcga
     3841 aacgccgcgc gggcgcctcg ccgtcttcgt ccgcgtcgct cgcgcttttg ccccaacggg
     3901 cgcgccgcgc ggagatgtaa agtgccggca cgatcatcac gccgaccaac gatttgacga
     3961 tcgccagcac gaccgggaat ccgtcgatct gggcgacgat caacagtgca cctatcagca
     4021 ggtactcgac gcccagcagc agattcaaca cacgccagtc ttccacaatg accaagagac
     4081 tgactgtaat gaccagcgca gtcaggatgg tcgtggacgg cagaccggat agtgcagaga
     4141 gtaagtcggt catcgctacc gaatcgcccc ggtcaagacg acgtaggcag cgaacaggat
     4201 gagcagcgcc cacacgaccg cgccttcacc ttcgatgacc gccgacagtc ggttgagggc
     4261 gctcgcgacc acgttggcga ggcgaaagct gagatgatac agccagtcaa ggttgagcag
     4321 acgggttgga ttgacgtgca tgctgatgcg tcgctcgcgc cgcagccact ccagcgtcac
     4381 ggccagcgca aggggcaaga tgaacagcaa gctgccagga atccccatgt ccggcagaac
     4441 cacggcgaag gcggggggtg tgatagcagg gacgacccgc cgggcgatag ccggtagcag
     4501 aaacgccggc agcgcgacca gggtgattgc cgacgcaagg ctggcgacgt cccgccagct
     4561 ccggggtatt ctggatgggt tgggtgtcgt caggagacga gccattgtgg cgaaagccag
     4621 cgtctcgccg gccaccacgc agaccagcag caatacattt ccaccccatg caatggctga
     4681 ggccaggctg gtgcgcacgg cgaagccagc cgaggctggt atacccacca gcgtcgcaac
     4741 ggcgagcgcc tgccccaacg accaccacag cgcgcgccgg tgaaaaccgt gattcaggct
     4801 gagcgtcacg atcgccaacg cccaggaaac cgcgccggcc gataggatgg cttgaggagc
     4861 ggtcggggtg cgtccggcga tgccggaaag caccacaatt cccaacgtgc tcaacgacaa
     4921 ggaaggcaac gcgtcctgcg aggcgctgcg ccaccaagcc agcgcgccgc ctgccaggac
     4981 gctgagcgcc gcaaaccaga tcgcggcggg ttcttggggc agcgcgtcca ggcttgccat
     5041 ccgaatcaag agatgtaatc cggccaatgg aggaagcacg tacaacaatg caactcgccc
     5101 aggcacatcc tcgcgctcgg ccggcagcca ttggctgaag ggataaagcc ccagccgtaa
     5161 aacggcggcg agcgccagga attggcgcgc cccctcaggg agttgggtca ggtgccaata
     5221 ctgactctcg ttgtcctggt taatcgcgag cgcggcgatc cacaggcaga gcgtcgccgc
     5281 gatgttgaat ccgacagcga aagctgcacg ccggccggca gcggactcgt cgccctgcat
     5341 cagtgccgca atcacgaaga acgcgtcgaa cagtccccag gccagcgcca gtgtgagcac
     5401 attggccgag aaggcagccg ccaccaggcc ggcagtcatg cccagcgcca gtgcgcgtgg
     5461 tccgaagcgg cgccggccgg ggtaagcgat accggcaagc gctgtcgccc cacaggcgag
     5521 tgtcgccaca agaccgatca tccagtctac acgatcgacg cggagtgcca ccggcgtgcc
     5581 gaacacggat gtaggctgcc aggacgatat cagtgtctcg cgaaccggga gatcggggac
     5641 ggcgagcagc atgatcgcta ccagagccag gagcgctgca accgacgcca ccgcatcgta
     5701 gtgcgcgaaa cgtgcggcgc ggcagagcag caaggcaagc actccaccga gcaggatcaa
     5761 gatcgggacc aggggaagaa tcgacatggg ggtctgaagc tgcaccattg taacgcgctc
     5821 ggcgcgatcg cgcaagaaca ccgtgatagg cgcgctgtcg agaaatttcc gagcggtgat
     5881 tgttcgaaag tgctcacatg cccgttcctt gacgaacggc gagcgtgcca aacgaacgca
     5941 atcatcggtt tcctggcgag tgacgattga tgctataatc gcgatgctcc ctgctcgtga
     6001 cgccgggtga ttcgttcacg atcatgtcca ttcagttgcc ggacggctcc attctcagtt
     6061 tctcgcttcc gcagcggttt gacagcgccg tcatcgtcgc cacctatgtg ctgtcggcgg
     6121 tgctcgtgtt cgtcatttcg ctgcccagct ttcgcgggct gcgccgaggc cagtgggcac
     6181 ttctggcatg ccttgcggtg atcagtgttc cgctcaacgc cattctgctc gtgcctgcgt
     6241 ctgcgttggc gtccagcacg ctcgcgccgc tgctcagcaa gctcgcgccg gcgccgctgc
     6301 tcggttggtt ctgcatgctg ctggccggcg catggctggg cgccggaccg gcaatgctcg
     6361 tcggattcac cggaggtctg ggcagatggc tgctggcggg cggtcgcttc acacagccgg
     6421 ccgaaatggc gctgatcgcc gctgccgccg cctttttcat gcagcagaac tatcgcggcc
     6481 gtctggccag cgcggtgcgg aagccgctgg tcgccggtct gatcgccggg cttctcgccg
     6541 catttatctc ggtgttcgcc gtcttcgcct cgatgccggg cgaggcgccg accgctgccg
     6601 attacacgct gtccactgtg gggtatgcga cgctcctcgc gctgggcgaa ggtctcatca
     6661 attctctact ggttcaactg gcctgcctgg ctttcccgtc catgcgtcgc atggtcagtg
     6721 cggcgagggc cccgttctac gcacgcagcc tcagccgtcg cctatggacg tccatattgc
     6781 ccatcacgtt cgtcaccatt accgtccagg tcgtcgtcgt cggcttgacg gcgctgaacg
     6841 tggcttaccg ccaggctgag acacagttgt cgcgttacac tcaggcgacg gcggacctgc
     6901 tcgatttgtt ctttctggat ggagaggcga tcttgtttca gtttgcccag gacggcagac
     6961 tgcaaagcga tgatgtacgt gcccggcaag cctatcttga atcggcgctg cgctctatcc
     7021 ctttcttcga cgagttgctg ctcgtggatg cgaatggtca agtgttggcc gcggcgcgct
     7081 tgccggctgt gggtgacgag tcaccggctc aggtcacccc acaagaggaa agccgcctgg
     7141 agcgcactct gcagaccggc gcgccgcagc gcaccagcgt gtatgacgat gggcaaggcc
     7201 gccctatgat gtcgtacatt cagccgagca cttccgtcgt tccaccgggc ggcgccgtgg
     7261 taggccggac tcgactgtcc accaacccgc gcattggcgc tatcaggcgc accctaccca
     7321 acacattgga cgcgggtctg ggctatgtgg tcgatgacaa ttccctcatt accattcatc
     7381 ccgatcccga cgctgagagg cgccagtggc tgtttgatcg gagccaggcc gcgatcggcg
     7441 acagcggaag cgcctacgtg gaccggtatg cggacggctc gcggcgtgtc atcgtggtgc
     7501 gggagatcgc gaacgcggcc tggcaggtgg caaccgagat gccctattcc aatatcgtgc
     7561 gcgccgcggg tgagatctct gtgccgctga tcggcatgtt cgccgtgatc atgatcggcg
     7621 cgttcgtgct gatccccctc atcagccggc gtatcacgca gccactggta cacctgacgc
     7681 aagccgccgg gcgcatcgcc caaggcgaat tggaccgccc ggtcaccgag gcgggagaag
     7741 acgaggtggg gcagctcggc gtggctttcg aaggcatgcg ccagagcctg aaaggccggc
     7801 tcgaggatct ctcactcctg cttcgtgtga gccggtcggt cgccgattca ctcgacctgg
     7861 aacgcggtat tcctccgatc ctggaagctg caatgcaggt gacgtcgtca cagggtcagc
     7921 cgccggcggc gcgcacggcc cgcctgctcg tgttcgacga ggacggcaag ccgttgcgcg
     7981 cgctgacgtg cggcgagggg ccggcacagc taacgcctct cgacgaagcg ctggcgacta
     8041 tcaccgtgcg ggacgagcgg ccgctcatca tcgagaacgt cgcccgatcg cgcggcgcgc
     8101 tcgatccgaa actggccggc cccggcgtgc gcgccattgt cacgcttccc ctgtggcgat
     8161 ccaaccgcgc tctgggcgtc atgtggctgg gatatcccga ggcgcgccga ttctcagatt
     8221 cagaggtcgg cttgctggtg acgctggccg gacaggcggc cgtttttctc gagaatgtcg
     8281 ggttgtatga tgccgcggaa ggcgggcgga agcggctgca ggccgtgctc agctcgacca
     8341 ttgacgccgt gttggtgacg gatagccaga accgcatcct gttgtgcaac ccggcggccg
     8401 aagccgcttt cggactctca tccggcgatg ccactggccg tccagccggc gaggtgatta
     8461 cggatcccat cgtcgtcgat ctcttgacag aacgcggcga tccggtcgtg cgcacggcgg
     8521 aagtgctgat gccggacggg cggacgctgt acggttcggc gtcgtccatc ttgtccgggg
     8581 acaacatccg gatcggccgc gtggctgtgc tgagggacat cacgcacctc aaagagcttg
     8641 acgcgatgaa gaccgagttc gtcgcgacgg tcagccacga cctgcgctcc ccgctcacgt
     8701 acatgcgcgg ctacgtgacg atgatcccga tggtcggcca agtggagacc aaacagcagg
     8761 actatctcga caagatcacg cgcggcatcg agcagatgac gaccctgatc gacgacctgc
     8821 tcgatctggg ccgaatcgag gcgggcattg gcctggtgcg cgagccgacg cgcctggccg
     8881 acatcgcccg cgaggccgtc gagtcgatgc agccgcaggc gcttgcgcgg ggtctgaatc
     8941 tgcgcctggt cgaatcggtc cccggcgcat tgcgcggcga caggcagttg ctcaagcagg
     9001 ccatctcgaa tctgatcgat aacgccgtca agtacacgcc cgcgggcggc tcggtggagg
     9061 tcgctgtgga cgagcgtgat agctgggcca tcgtgcgggt gaccgacaac ggcatcggca
     9121 tcgccgccgt cgatcagccg cgcctgttcg agaaattcta tcgcgtcaag cgtcgtgaca
     9181 cgatcgacat tcgcggcacc gggttggggc tggctatcgt caaatcgatc gccgaatggc
     9241 atcacgggcg cgtgtgggtc gagagccact tgggcaaggg ggcgacgttc tacatcgcgc
     9301 tgccgacgga aggataagcg gtcgcccgct tccgcagtcg tcccgtgcgg tttgccacaa
     9361 tcggtccact atggtaaaat ctgtgccgct ttgaagacaa acggcgaccg gcgtgtccgg
     9421 ttgtctcgcg tctttccgct cttgacattc ggatcaaggg caatcccatg gaaaggaggt
     9481 ttatcgcgag catgcgccca tatgaactca tgttcatcgt ccggcccgag gtcgaaggcg
     9541 gggacctgaa tgttgtcgtc gacagagtca agagtctggt gaccggcggc ggcggccagg
     9601 tgaccgaagt caacgcctgg gggcgacgcc aactggccta tccgatccac aagatcatgg
     9661 aaggccagta ctttttcgtg cgcgcccaat tgccggcgca cgccctcggc ggactggaac
     9721 gcgatctacg gctcaccgag cagattgtgc gttatttgat cgtccgcgcc gacgagtgaa
     9781 gaagcgctac ggacgcgaag cgaggtagat cgatgacacg cggcttgaat aaggtcatga
     9841 ttatcggcca gttggggcgt gacccggaaa tgcgctatac gccgagtgga cgccccgtca
     9901 cttcgttcag tctggcttcc aatcggacct ggattaccag cgaaggagag cggcgcgaag
     9961 aaaccgagtg gttcaacgta gtcacctggg gcggtcttgc cgaaatctgc aagcagcacc
    10021 tgcacaaagg tcagacgatc tatgtggagg gccggctcca gacgcgcggc tgggaagatc
    10081 aagaaggcaa gaaacgcttc cgcaccgaga tcgtcgccag cgaaatgatc atgctgggag
    10141 atcggcgcgg cgaatcggcg gacgagagca tcggcgaagg cgttccggcc gacgaagact
    10201 ttccattctg actttcacgc ccggctcgac gcctcgcgcc ggacccgatt ctagggagga
    10261 ttcaccatgg tcgaagagtt gaggagcgct gagcctggca acagcgcgtc cgaagaacga
    10321 agcggcgcgg cgcccggcgc ccggggcggc tcgggtccgc gcagatcgcg cggtgggggt
    10381 ggcaagcgct ttcagggcaa gcccaaggtt tgcctgttct gcgtcgagaa aggccagcac
    10441 attgattaca agcaggccga tctgctgcgt cgcttcatga acgaaagcgg caagattcgg
    10501 ccgcgccgcc agacgggcac gtgcgcccgc caccagcgcc tgctggcggt cgccatcaag
    10561 cgcgcacggc atctggcgct gattccgttc acgacacgcc cggcgcgcta gaactatcga
    10621 cgaatcgcgc ttcagtgcct cagcgcctca gcgatttatg tccaagcgtc cacaggcatc
    10681 gccacttcct aaggaacttt cccgtaagca tatttcgcgc gcccagcgcg acgcgcagcg
    10741 aacccgcatc ctgctgttcg ccgtgggcgg agccgtcgtc gttgcgatcc tgctgatcgc
    10801 cttcggcttt gtgcgcgaga ccttcatcac gccgaacgaa ccggtggcaa tcgtaggcaa
    10861 tgagcagatc ttgactcgac agttccagca gcgcgtccgt ctgaaccgct atcaactgct
    10921 ccagcaatac agcctgcttc agagcttcgg ttcgaacgac agtgcggcgc aggtgctgca
    10981 gcaactgaac gattcgatca gcctcggctc gcaggcgatc aacgcgctcg tcaacgaggc
    11041 gatctatcgg caggctgcgc ccagtctggg cgtcgccgtc acgcaagacg aggtggacaa
    11101 gctcatcgag gaagggtttg gctattaccg caacccgccg acgccggcgc cgacgagcac
    11161 gccgcgcccc acgccgacag tgccgccgga tgccacaccg acgccgaacg cgacggcaac
    11221 agcaactgcc acgccgcgcc cgacgtccac ccccatcact gccgaaggat tccagtcgct
    11281 gtaccaagcc cgcttgagcg agctggccgg ctttggattc aacgaggcag acttccggcg
    11341 gctcttcgag atgcaactga tctcagagcg cgtgcaggac atcattgtca gcgacgtgcc
    11401 gacgatgacg gagcaggcga ccttccagtt tattcgggcg ctctcccaat ccgacatcga
    11461 agcggtgcag caggctatcg cggaggaggg tttcgatgcc gtctacgccc aggcgttgtc
    11521 gcagaccctg ccgatttcaa acgtgctcgg atcggagctg ccgttcctgc ctaaggaaga
    11581 cctgatcgaa tcacccgcgt tcggcgcggc ggtcgccgat gccgtgttca ccacaccgat
    11641 cagcgacatg ctcgctatcg ttacggatcc cgcaggcgtg tatttctacg tcggcagggt
    11701 gccggcacgc gaggtgcgcg agctagcgcc ggatgcgctc gagcgccggc aggctgctgc
    11761 cattgaggac tggttgactc gacagcgcga gcagttgaat gcccaggtat tgacctggga
    11821 agatcgcgtg ccgaacgatc cgtgagctac atcctgagcg cagccgaagg actcacggcg
    11881 tgcaaaacag actcgacctc ctgcaagcgg tagcctgcgg gaggttttct tttacggtgt
    11941 cacgttcgga cccggcgtat tcgtcggcgg cggcaccgtc gtcggtggcg gctccgtggt
    12001 cagcgtcggg acggttggcg acggagtatc agttgccggc ggatcgtcga aagtgggtgt
    12061 cggcgtcggc gactgggcag gcggcgtttc ggtcggggga ggggaagggg agggggcccc
    12121 tggcgttgtc ggctcgggcg ttacgagggt ctccaccggc gttggcgtcg gcgtgccggt
    12181 gggcgagggc gatggagtcg gcgtgggagt cggggtgaac ggtatggcca cggtaggaat
    12241 cggcaggcgc ggcacataca agatttggcc gacgcggatc ttgtcgctcg gcagacaatt
    12301 ggccagcatc agcgcctgta cggtgctgcc cgtcgcgcgg gcgatcgacg acagtgtatc
    12361 gcccggtcgc accctgtaga tcgtccagcc caacggcgga ccgcacacgg tcggccggat
    12421 gacgacggtg gcgatcaccg cgaccgtcgg cgtcagcgtg gggggcgcag gtacgaacag
    12481 gatcatgttc ggcgtcacga aggcggcgac gaggcaattg ttgacctgta gcacaacggg
    12541 atcgatgccg tagcgtgcgg cgatgccagg caagtcttcg ccctcggcga cgaggtggtc
    12601 gatccagcca ggcggtcgcg ggcacgtgct agtcggcacg agtgtcggcg tccagacgac
    12661 agtcgggctt gtggtgagcg cagccgaagc atcgggcaat gccgacggtc ctgaaatagg
    12721 aggtgctgcc gaggcagtag gggatgggac gctggtcagc gaggcgatcc tcaggtcgcc
    12781 cgcggtgaga accagaccgc tggcgatgag ggcgatgacg acggcgccgc tcaccaggcc
    12841 gccgaggaac tgctgaaggg tcttcacacg ccagccccgt taccgcctgc tgaaccaaga
    12901 cgggcaggcg caaggcctgc ccctacgagt cttcacgctc cagctccgtt gccgccagtt
    12961 gaaccgccga cgggaatgat gaacgtaaac gtggagcctt tgcccatatc gctgtcgacc
    13021 cagatgcggc cgccgtgcgc ttccaccaac gcgcgtgcga tggacatgcc gaccccggtc
    13081 tcgcccaatc ccgcgatgag cgggttgtcg gcgcgataca tgcggttgaa cacacgctgg
    13141 cgatccagag gatcgatgcc gccgccgcag tcggtgacgc agatgagcac gaagtccggc
    13201 gcctcggggt tcatgtgcgc gccgaccatg acttcgccgc cgggcggtgt gacggcgctg
    13261 gcgttcgaca acaggtgcga gagaatctgg tgcatgttat cgcggtcggc ctgtatctga
    13321 ggcagatcct cgtcgagatc gagttggata gtgatgccgc gctcccggaa ctgcgccgac
    13381 gagctcatga tggcttcttc aatcacttcg acggcgtcga tcggctccgg ttcgagcttg
    13441 atccggccgg cgtcgatggc ggcgacgccg atcaggtcat tgagcaagcc gcccatgcgc
    13501 tcgacattgg ccttcacgcg ctgcaggaac tgccgctgca tggcgcccag gatgccgacg
    13561 gattcgccaa gcagcaggtc ggtgtagccc gcgattgagg tcatgggcgt gcgcagctct
    13621 tgcgcgatcg aggcgatgac ttcttcgcgc ggcgacccgt ctgggcctgc cgcgccgcgt
    13681 agtgtcacga caatgccgtt gtacgtttcg ccgctgccat cctggaatgg cgtcaactcg
    13741 gcgtggagcg gcttgccggc ggcactgacg tcgatgtgga agggtggctc cccggcggcg
    13801 gtcggattcg gcgctcgcgt cagcaggtcg agcgcctcac gccaccgccg atcgctcagc
    13861 acgccgtgga tcggttggcc aatcagcgcg ccgcgcggct ttccgatcag gcgctgtgcc
    13921 tgatccgtga cgagggtgac tcgacccagc gtgtcgctga cgagaacgcc cggctcgccg
    13981 ccctcggccg gcgctgccgt cgccagcgcc ttgacggcct gtcgctccgc gtcgtcgcgc
    14041 aagcgggtga tttgctgctg cgcgtgtttg agctgcgttt ccagcgcctg ttgcagggcg
    14101 gccagttgag cggcctcttc gcgcgcatct cggagctggg cttccaacgt gttcctctcg
    14161 tccgtgagtc gccgcgcctc gtcctcccac accgaggatg ggcttggcgg aagtgccctc
    14221 ttttcggcct cctgaacctc gacgagcgcg gcgagctctt gcgcgcgacg atcggcctgt
    14281 cccgcgcgct gctccgcgac aagcagcgcc gccttgagct gctcgatttc ggcctgcgcc
    14341 tgttgagccg ctccagccac tgccgcccgc tgtgcattca aatcgatgga ctgttgatgc
    14401 agcgtctcgc tgatctccga cgcgcgtttt tgcagcgtgt cgatttgctg ccggctatat
    14461 agcgtcgcgg aaagcaactc gccgtaggtt cgcgcctgct gtgcgtccga cgcgctgata
    14521 gcgcgtccac tgccgggatt cccgatcaga agaacgccga ggaccgtatg atggctgacg
    14581 agtggctgga tcaacaatgg cccggacacg aacgaaccca tcaggccgaa cagcccggag
    14641 acgtccgggg catcgtgctg cggtccgatg atcacagcgc gctggcggtc gatcgcacgc
    14701 ctgactgccg gatggtcttc aagtgcaaat acggcgcccg gcgtcggcgc cgcgccggga
    14761 tgatggatcg cgaccaactc gacaaggttg gctgccggac cgggcaggcc gagtgctgcg
    14821 aggtctgcgc gcaagctggc ggcgacggca gcgcatatct tttggtacgt caacggcaga
    14881 tccgcgctcg tgctcacctg gcgaatggta tcaactacgg cccacacgtc ggatgcccat
    14941 gatgcggatc gggcaggggc aagccctgcc ccggcaggcg caggtatcgc ttcgtgccgc
    15001 gattcgacgt attcacgata gaccacggcc gccagaagcg gataggcggc aagttgagcg
    15061 aagcgcaccc agccggcgat atcggtgctc gcctcgggcg ccacggcttg caggaagtgt
    15121 ccgcccgcca ggatgacgaa ggcgatcacg acgaaggccc actgcccacg cctacggatc
    15181 agcgtcagca ccgccgcgac ggcggtgagc gcgagcgacc aggcctgcca gccccgatcc
    15241 tgttccgaat agaccatcgt cgcctggctg cttgccgcat accacgctgg ggcgacggcg
    15301 acgtacgcaa tgagcgcagc ggcagcgctg ccgccggcaa agatcaggcc gaggcgcgtg
    15361 tcatcggtga ccagcggcac gaaggctaag gcgagaagcc cgatgccgat cacgtcgatg
    15421 gcgcgatcga gcggcgggac gaggctgcgc ggtggaacga tgtcgctgtt ctccagcaca
    15481 acggcgagca gcaagaggcc ctggcccaga agcatgccgc ccgccgccca gtggagacgg
    15541 gggagcgggt tcgccgagcc gtgcgccctg atctgtccga cggctgcgta cagcatgaac
    15601 gtcagggaaa acagcgtaac caggtggaac accaggctgc cgggtggctg ggtgaggcgc
    15661 tcgacgatct gcgtcatcga tatcatgtca acctgtcctg atacggatta tacaacgagg
    15721 ctgagcaggg gtacagttac ggttaggtga acaaaggagc gacatgcaga attgtgaaca
    15781 gattcacaac acgctcacgt gatcaagtct ctcggaagaa agtcgacccg gttcgcatac
    15841 accagcccga cgttaccctc gtcaaagtcg attcgcgtga tccccgcatt attcaagaga
    15901 aaccagtagc catccgctgc cggtgcgttc agcaaggcca gcagaaactg ccggtagaag
    15961 ccgccgtggc tgatcacagc cacgcggtcg ttcgtgctgc cgtgccgcac ggtgagatcg
    16021 ctgagaaagc gccgtgcgcg aatcggccac tgctcctcgt cttcgggagg gcgattccac
    16081 catcccgcct cgcccaatcg gttcggcagc acgaggttgg gaaagcgcgc cgcgaactcg
    16141 gcgcgattga gcccggcctg accgatcttc tcctccgtct cctcgccgtc gaggtagagg
    16201 ccgccgttct cgtggatgtc ttcccacgcc atcagcggga gtcccaatgc ttcggcgacg
    16261 attgagccgg tctgcacggc gcgtaccatc aggctggtgt agacgtgagt gataccgaag
    16321 cctgagacat tgtgtggatc ccatccgttt gccacagcgc cgggattggc ttgccttagg
    16381 aaccgggcaa gatactcggc ctgccgtatg ccgatgtcgg tcaattccgg atcgtgactc
    16441 cgacctgacc aggagccggt acgatcgtac agtgcgttgt tcgccgattg gctgtggcgg
    16501 atgaagtaga gctgcattca gtgggcctca ccgtcagtgc ttcgtgactc agcgctcaca
    16561 gcgtatcaag tgtgtgcagc caacgggtac aactctccgt atttcgcccg caggtattcg
    16621 agatagggct caatcgtcat cgacttgccg gtggcgcgct cgaccagttc ggacgcggtg
    16681 aacttgctgc cgtgccgata cacattgtcc gtcagccagc cgtgcaacgc gccgaattcg
    16741 ccctgcgcga tctggccagg tatctccgga cgtgcagcga gcgccgcctt gtaaaactgc
    16801 gcgctcagga tgttgcccag cgtgtagccc tggaactggc cgccgacgac gctgccgaac
    16861 cagtggacgt cctgtagcac gccgtcgcga tcgtccggcg cgtggaggcc caggtcggcc
    16921 tggtagcgcg cgtgccacgc atcgggcagg tccctaacag ccagagtgcc ttcgagcatc
    16981 tgcaattcga ggtcgaagcg gatcatcacg tgcagattgt aggtcacctc gtcggcgtcg
    17041 gtgcggatga gcgatggctg cactctgttg atcgcgcgat agaaggtgtc gggggtgacc
    17101 gctcgaagtt gctcagggaa ggtctcctga agcttgggat agaagaacgt ccagaaattg
    17161 cggctgcgcc cgaccatgtt ctcccagagg cgcgactggc tttcgtgaac gccagccgac
    17221 gttccgtgcg ccaacggcgt gccctcgtac gccatatgga taccctgctc atacatcgcg
    17281 tggccggact cgtgcattgt gctgaacaag gcatcggtca ggtcgttttc cttgaagcgc
    17341 gtcgtgatgc gcacgtcgcc gagcgagaat ttcgtcataa aggggtggtg cgtcttatcc
    17401 tgtcgccctc gagtgaaatc gtacccgtat cgcttgatga catccagtcc gaaagcgagc
    17461 tgcctgtctt cggggacgcg atgtcgaagg cacgaatcgt ctgcgacggg ttgggctgca
    17521 atcgcctcga cgagcggcac gagctgggcg cgcaggtcgg cgaacaaggc tcgcaaggtc
    17581 gacgccttca tgccgtagtc tgcctcgtcg atcagcggat cggcaatgtg ctcgtagccg
    17641 ggaaagtagc cggccatctg gcggctcaag tcgaccgtct tctccagcag cggctgcatg
    17701 cgccggaagt cgtttgccgg gcgtgcttcc gtccatgcct ggtacgatgc tgccgcgtgc
    17761 tcttccagct cggcgacaaa ctccggcggc accttcaccg accgctcgta ttcccggcgc
    17821 gtcacgcgga tcaatgaggc gtcgtccgag tcgtatggca ggccctcctc gaaggggcgc
    17881 aaatcgccca gcagcttgcc gatcgccggg tcgataaact tctcgtgcgc gatgcggccc
    17941 agcgttgccc tctgccgccc gcgcgcctcg gcgccacctg gcggcatgta ggtctcttga
    18001 tcccacccca gcagcgcgtt agcggatcgg atgtcactga tttccatcaa acgcatcttt
    18061 agctctcgta gtttcgcctc cattcatctt ctccagtcga ctgagtgtat ctgctctgac
    18121 gcccggacct cgcgtccggc tgcaggcagc ggcgcaattt tagggtggaa aagcggatgg
    18181 gtcaaaacgg ggtcggccaa atcgtgtatc gtgttgcctg aggatgactg tcccgatgaa
    18241 gtgaggcttg ggatggtgcg tcgcgatgag aggctgcccc gttcaatccc acccgtgctc
    18301 cagtcgaaag ccttctggat cgcagatggc acacttcttg aacttgagta tgccatgcgg
    18361 gcacttttcg aagtcttcag tgtaaggacg cggatcgtgc gcgataagcg gcgtgaagac
    18421 gtccgattcc cggcttgggc ttcggccgat gacgccgtcc tgcgaccgcg agcgccgctt
    18481 ggagcgctgc gcacgcgcgg cgcgaatctg ctgagcaata tcctctggtg aggccgggag
    18541 gtgaccatgc tgatcgagaa atgcagcgat gactttgaaa gaaggtggtt cgcttgaggt
    18601 gaagtgcttc tgcacactca cgcagaactg gagggcttga gcctcgccag tgtgcaagcg
    18661 tgcggcgatt cgtctgatgt gattttccga cattgtgccc atcctccaac tgtgggcacc
    18721 gatgccccca gtcggtgctc gcatggctgc cgtccagaaa caccggggtg cttatattct
    18781 acaggtgagc gccgcaaaga tcaatctgcc ttacgccgtt cggacgtctt tgtgacgaac
    18841 tgaccacact tatggatcag aaaccccgta taacttctgg tatatcgcca aatgctcgcg
    18901 cacacgcagc aaataggcac gcgtctcgtc gagtgtgatg gtctcgagaa ataggtcggg
    18961 gtcgcccttt gccgcgtcac gccagcgctg cgcgttgccc gggccgccgt tgtacccggc
    19021 gagcgcggcg tacacatccc cgtcgaagcc gtcgcgcacg ttggccagat accatgtgcc
    19081 gaaggtcacg ttgacggtgg gcttgtacag atcggctgtg ctgtaatcgg gccagcccag
    19141 gcgcgatgcg atgaattcac cggtggacgg tatgatctgc atcaatccgt tggcgaacgc
    19201 cctcgagacg gcgatgcctt cgaacaggct ctcctgccga atcagtgcga aaacgagaag
    19261 cggatcgagc ccgtgctgct cggcttcggg aacgacgaga tcggcgtagt acgtaggata
    19321 ggataagcgt gccacgaagg ccggcgcatt gtccggcgtt ttcgcaggag agatcgcgat
    19381 cagacgagcg gccgcgatga gggacgatcg gtacaggccg atgtcgcgcc agtagaggga
    19441 cagcgcatac agcgcgaccg ggtcgtcgtt atgcgcggcg cgaaggtcgt cgaactcgct
    19501 cctggcctcg tcgacccagc ccagccgcca caactcatct ccccgccgaa aacggccatc
    19561 ctcaaggatc gattccggca gcgcaccgat accggccgca tcctcccggc ctgtccactc
    19621 cgcgagccat gcttcggcct cggctcgctc ggctgcttcg tcgaattcca gatcgaacgg
    19681 aaccggctgg agggtagggc ggtcgtcgcg cagttctgcg gcgcggatgc cataatagcc
    19741 gtagggcttg gctcgtgcgg cgaggtcgag cagcgtccgt cctcgtgcgt ctccgccgtg
    19801 ctgcgcgagc ttgccctgcc acagcagggc ggccggatag cggtcggcag tcggataggt
    19861 gtcgctcaac acgcgccacg cttcgactgc cggttccgcg cggcctgcgc gataggcatt
    19921 gacgccggcg ccaaacaagg cgtcgggggc cagcgtgtgc gagggatatg actcttgcag
    19981 ggatcgaaag acctcggcgg ctcggtcgaa ttcgccggcg cgctcaagca gaattccggc
    20041 gcgctgcagc gcattcggcg cgagcgcgtc gcgcggagtg tcctcggcga acttcgtcag
    20101 cgtcgcggct gcgccttcga gatcgtcgcc ctgcgactgc gcgatcgctt tgtcgatcca
    20161 ggcttgcgcc cacagcggag actggggatg atcctcgatc agagcgtcga actgttgtat
    20221 cgcggcgcgc gtgttgcctg cggcgcggtg cgacatcgcc atgtagtaat gtgcgtcggc
    20281 atggtcctcc accgagtcaa tgaattcacc cagcgcggcg acggcggcgt cgtactgtct
    20341 cgcgtaatag tcgacgaggc cgcgctgaaa gaagtcgacc gggatgccgt tgccgaccag
    20401 ggcgacgagc gcctggtagg cgctatccgt cttcggatac gtgctgacca agtcggcgaa
    20461 tacgtcgtag ccagcggcat cgcccgaatc gatcagcgtc tggccgagct ggaggagcac
    20521 gccggcccga tacgcatcaa tctgcgcgaa gcccaggatg gcgcgatact gagcgatggc
    20581 atcgtcgtag tcgccgcgca gacggtagac gagcgcgagc ttttcgcgct gccgcgcggc
    20641 gaccgggttg ttgggcgcgg cggcgatggc ctcccgataa gcaacgacgg cggcctcggt
    20701 gtctcccgct tggttgagcg cgtcgccgat tcgttcctgg acgtaggatc cgatcacgct
    20761 gccacgcaga tcgagatatt cccggtaggc gtcaaccgcg cccgtgaagt tgccggacgc
    20821 gtgacgcgcg tcgccgagca gaaaccaggc atcgccgagc agcgcgctgt ccggatagcg
    20881 atcgatgaac tgtccgagca tattctcggc agcgacgagg tccccggctc gcagcgacgc
    20941 cacgcctgca ccgaacagcg ctgcttccac ctcggacgcc gcgcccccgt catccagaat
    21001 ggccccatat tcggcgatgg cagactcaaa gtctccgttg cgcatgtggc gtgcggcatc
    21061 gccaagacgc acggccggca gcggcgtggg cgtcggcgtc gctgtcggcg atggtgtggg
    21121 cgtctcggtt ggcgcgccag tcgggtctgc gtctggcgtc gtgaaaaccg gcacggcggt
    21181 cgaaagcggt ggtgcgatcg gcgacgtgtc ggatggcggg cttgtcgcac acgcggccgc
    21241 ggcgaacgtc agcccgatcg cggtcagcac agaaagacgt cgttgtgcac ctggcatgat
    21301 ttgtcctcgg ccgcgccatt atcggggacg atgcgccgcg tgtcaatgag agaacaggca
    21361 cggggacggc gctcacgcat tgacagatga tcggggctat gctagacttg tcgttgttcc
    21421 ccgctgtcga tgcgcttttc ggttgtcgct ctcgcgtcga gagcgatttt tgtgcagggg
    21481 ttgtgatgtc cgagtcgatc gagcgcacca tcgagtggca cgaatcgccc ggcggtgcgg
    21541 gagggcgcgg cgtcgtcaag atgatcgacc agcgtctgct gccgcacgag gtcgtcgttc
    21601 ggtcatacac cgactatcgt gacgtggcgg aggcgattcg cacgatggtg attcgcggcg
    21661 cgcccgcgat tggcgcggcg gcggccttcg ggatggcgtt ggccgcattg cacagccagg
    21721 ccgagagccg actcgatttg ctgcgcgatc tggagatcgc cgcgaatgtg ctgcgcgagt
    21781 cgcgcccgac tgcggtcaac ctgttctggg cgatcgagcg catgctgcgc gcggcggtcg
    21841 acgagtcgct ggactcgacc gccgacgtgc gcgagcgcct gatacgcgaa gcgcagcaac
    21901 tggctgacga ggacgtcgac atcaaccggc gcatggccga acatggcgcg acgctgattc
    21961 gcgacggcga cacgattctt caccactgca acacgggcgc gctcgcgacg gtggattggg
    22021 gcacggcgct cggcgccatc cgcatggcgc acctgcaggg gaagcgcgtt cacgtgctgg
    22081 tcgacgaaac tcgcccgcgg ctccagggcg cccggctgac ggcctgggag ctgatgcgct
    22141 ggggcatccc catgacgttg atcgccgaca acgctgccgg gcactacatg cgcaccggca
    22201 aggtggatat caccctcgtc ggctcggacc gcacggcggc aaacggcgac gtggcgaaca
    22261 agattggcac ctataagctg gccgtgttgg cgaaggagaa cgggattcct ttctacccgg
    22321 tcgtgccgac ctcgacgatc gatctcaacc tgaaaagcgg cgacgagatt cctatcgagg
    22381 agcgcgacga gcgcgaggtg acggagatcg acggcgtgcg tatcgcgccg gtgggcgtga
    22441 aggtgggcaa tccggccttc gacgtcacgc cgcatcgcta cgtcaccgga atcgtgaccg
    22501 agaacggcat tgcctatccg ccattcgagc tgagcctgcg aaagatggtt gagcgtaaag
    22561 cgtgaaacgt aaaacgtaaa ttacgtttta ctctttacgt tttactcctt acgttttgcg
    22621 tctctattga gcctatgtct gagaacttgc ccgtcgtcat cctgtgcggc ggcgccagca
    22681 cacgcatggg cggctcgggt ctcgacgacc gccccaagcc gctgatacac atcggcgagc
    22741 ggcccgtgct gtggcacatt atgaagatct acgccgcctt cggtcacacc gacttcgttt
    22801 tggcgctggg ctatcgcggt gagatgatca agcgttactt cctcgactac gagccaatga
    22861 gccgcgattt caccgtcgcg ctgggcgatg cgcggcgc
//
LOCUS       DBNHXB010000132         4903 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1703113, whole genome shotgun sequence.
ACCESSION   DBNHXB010000132 DBNHXB010000000
VERSION     DBNHXB010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1703113"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="AC162G_05100"
     CDS             complement(<1..293)
                     /locus_tag="AC162G_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075225.1"
                     /translation="MLSLTSRWLTRALAALYGFLGAILFLAPEWSAASFSWNISPFVA
                     MTIGGWCLGNAVFAWEGARIWRWSIVHPLLIYLWLFGLLETTVLITFSDRVIL"
     gene            complement(501..779)
                     /locus_tag="AC162G_05105"
     CDS             complement(501..779)
                     /locus_tag="AC162G_05105"
                     /inference="COORDINATES: protein motif:HMM:NF015630.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HIR5075226.1"
                     /translation="MTETAFEIRVPTDLLQFGLDQHEIQRRLVEWLVLSLFTEGHISS
                     GKAAHLLNMSRIELLALLRARGIAYINYTPDELAEEFAAIDQLKVTPQ"
     gene            complement(953..1363)
                     /locus_tag="AC162G_05110"
     CDS             complement(953..1363)
                     /locus_tag="AC162G_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR5075227.1"
                     /translation="MWEDALWRLTVSLDAEVLGFAYLEPKRHIPHITDLDGEEARTFG
                     EVLARVSRVLREETEAELIYIYVFGGGVPHLHVHLAPHRPGDALNTQIIRGEVVVEKM
                     ESGAGRSVSKEFLPLPEAEQRAVAQRVQQRLGSH"
     gene            complement(1453..2130)
                     /locus_tag="AC162G_05115"
     CDS             complement(1453..2130)
                     /locus_tag="AC162G_05115"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5075228.1"
                     /translation="MDRQQIADLQTSYDRVAAEYVQRIYHELDHKPLDRQLLDRFAAG
                     VHGLGPACDLGCGPGHVARYLSERGVDVFGIDLSPGMVEQARRLNPGIEFRQGDMLAL
                     DAKDETWGGIAAFYSIIHIPREEVISALSEMRRALRGGGLLLLAFHIGEEIVHREEWW
                     GHPVSLDFLLFQPEEMAGYLASAGFEIEEVITRPPYADVEYASHRAYIFAKKPVRLLS
                     LQATQSL"
     gene            2419..2763
                     /locus_tag="AC162G_05120"
     CDS             2419..2763
                     /locus_tag="AC162G_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075229.1"
                     /translation="MRRSPLTYSLTLALVAALLMISLIHLVALDTRALGTRAPHAHGA
                     ASHAPAPDLGDGCCLFVCTSLIMGLLIATPALFRLRGGMASAEPTTLATILLPLEPPP
                     RLAPASHRIRVA"
     gene            2906..3358
                     /locus_tag="AC162G_05125"
     CDS             2906..3358
                     /locus_tag="AC162G_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5075230.1"
                     /translation="MSSIALRARVVLLVSLALALAACGGTAPATSDTMSEEPQEVTMT
                     MQEFKFDPTEISARVGRPVKVTLVNKGTLLHDFSSMDAKVEVVSMQEGAAHDMGGMES
                     QMTMHMAVDASTTETLEFKPTEAGTYEFYCSVEGHKEAGMVGQLIVTP"
     gene            3423..>4903
                     /locus_tag="AC162G_05130"
     CDS             3423..>4903
                     /locus_tag="AC162G_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR5075231.1"
                     /translation="MKESTIQVDGLLTALCARNVEKALMKMPGVHHVSANYLNSTATV
                     HYDESQVSLAQLQAAVSDCGYVCAGEALPEHMQHAGHAEHAAAMPMDAGAHAAHAGHA
                     MPAAPPAAHAGHVAAGVMEDHAAHGGRPGMTAGEMERDMRNRFFVSLVLTVPVFLFSH
                     LATQILGLHIPLPFGIGDKVFGFILTTPVVLYGAWPFYVGARNGLRQGALNMSVLVSL
                     SVLTGYLFSVAATFLFDGEVFYEAAAMLVTFVLFGHWMEMRSRRGTSQAIQKLLQLAP
                     PKATVVRGGKEIELPTEQIVVGDVVIVKPGDKIAVDGVLIDGESSVDESMITGESLPA
                     KKKAGDPVIGATINKTGSFKFRATRIGADTALAQIVKMVQAAQNSKAPAQRLADRAAH
                     YLVLFAVFAGLGTFLLWYFVLAPQFMPADADRLVFSLTFAITVVVITCPDALGLATPT
                     AISVGTGLGAQHGILFKDATALEGAAKIDAVIFDKTGTLTKGEP"
BASE COUNT          969 a         1545 c         1484 g          905 t
ORIGIN      
        1 ttgaggatca ctctgtcgct aaaagtgatc agcaccgtcg tctccagcag cccaaacagc
       61 cagagatata tcagaagcgg gtgcacaatc gaccagcgcc agatgcgcgc gccttcccac
      121 gcgaagacag cattgccgag gcaccagccg ccgatggtca tcgcgacgaa cggcgagata
      181 ttccacgaaa agctcgccgc cgaccattcg ggcgcgagaa aaagaatcgc acccaggaag
      241 ccatacagcg cagccagtgc acgagtcagc caacgagagg tgagcgagag catggtgaac
      301 tcctttcacg cttgatggcc gagacttcgg aagtctgaga gctgttccgg cgcacgcatt
      361 ttaacccaaa aatggcctgc cgccggtttc ggcggcaggc gaatctttca gacctggatc
      421 actccgctaa gcgggcacgc acgctgctaa cgaccttccc gtcggtcgcg cccgagtcga
      481 cacccgattg gagaccacaa tcactgagga gtgaccttca actggtctat tgcggcgaac
      541 tcttcggcca gttcatcagg tgtgtaattg atgtaggcga tcccccgcgc tcggagtagg
      601 gccaagagct cgatgcggct catgttgagt agatgggcag ctttacccga agagatatga
      661 ccctcggtaa agagagacaa aaccagccac tcaaccaggc gacgctggat ctcgtgttgg
      721 tcaaggccga actgaaggag atcggtgggg acacgtattt caaaggctgt ctctgtcata
      781 ggccaatcct tctgccactg tggctagcat agtaatcata gcgcaaagga catactttgc
      841 caattcctcc accgttttcc gttttgccag gcagcggtca tcgcgattgg acagccagtt
      901 gcccgactgc ccgcacaaat ccctatatgg cggtatgaaa gcgactgtgc tatcagtgcg
      961 accccaggcg ctgctgtacc cgctgggcca ccgcccgctg ctcagcttcg ggcagcggca
     1021 gaaactcctt gctgacagat cgtcccgcgc cactctccat cttttcgaca acgacttcgc
     1081 cacgaatgat ctgggtattc aaggcatccc ctggccggtg cggcgccaag tgaacgtgaa
     1141 gatggggcac gccaccgcca aacacgtaga tgtaaatcag ctcggcctcc gtctcctccc
     1201 gaagcactcg actcacgcgc gccaggactt ctccgaacgt gcgcgcttcc tcgccgtcga
     1261 ggtctgtaat atgcggaata tgccgcttgg gttcaagata cgcgaacccg aggacttcgg
     1321 cgtcgaggct gaccgtaagt cgccagaggg catcttccca cacctggata cggagaagct
     1381 ccgcatcgcc agaagcgccg cgacagataa cacactccgg ttgagtttcg acgttagacg
     1441 tagtcctgtc tcctagagag attgggttgc ctggagtgaa agaagtctga ctggcttctt
     1501 tgcgaaaatg taagcccgat ggctcgcgta ttccacgtct gcgtaagggg gtctcgtaat
     1561 cacttcttcg atctcgaatc ccgccgacgc caggtaaccc gccatctctt ccggctggaa
     1621 gaggaggaaa tcgagcgaca ccggatgccc ccaccactct tcccggtgga cgatctcctc
     1681 gccgatgtgg aaggccagca acagcagccc tccccctcga agagcgcgcc tcatttcgct
     1741 cagggcggat atcacctctt cacgcgggat gtgaatgatg gaataaaagg cggcgatgcc
     1801 tccccaggtc tcgtctttgg catccagcgc cagcatgtcg ccctgccgaa actcaatgcc
     1861 gggattcagc cgccgcgcct gctcgaccat gcccggcgac aggtcgatcc cgaagacgtc
     1921 gacgccacgc tcgctcagat accgcgcgac gtgccccggc ccgcagccca gatcgcatgc
     1981 cggccccagg ccgtgaacgc cggcggcgaa ccgatccagc aactggcgat cgagcggctt
     2041 gtgatccagc tcgtgataga tgcgctgcac gtactctgcc gcgacgcgat cgtaactggt
     2101 ttgcaggtcg gctatctgtt gtcgatccac tcttattttc ctactgccaa gggaactgaa
     2161 atgctgcgcc gggcatctga atttgcccta tctggcgctg gagtcaggct ttagccggtt
     2221 ttgaccacca gatgttaccg cctaaaggct cgactccaaa attcgccaga ccatggttcg
     2281 ggaatagcac ggctacgcga tctcagtcta tgacagcccg tctccccagt caagtgttag
     2341 ggtcactccc ccaacgccgc gcacagcgaa tctgaactgt gatatgatta taaggtcgtt
     2401 tagccgggca ggcatcgcat gagacgatcc ccgctgactt atagcctcac gctggcgctc
     2461 gtcgcggcgc tgctgatgat cagcctgatt cacctggtcg cgctggacac acgcgcgctg
     2521 ggcacgcgcg cgcctcatgc gcacggcgcg gcgagccatg caccggcgcc tgatctgggc
     2581 gacgggtgtt gcctgttcgt gtgcacgtcg ctcatcatgg gcctgctgat cgcgactccg
     2641 gcgctcttcc gcctccgggg cggcatggcg tcggccgagc cgacgacgct ggccaccatt
     2701 ctcctgccac tggaaccccc tccccgactc gcgcctgcgt ctcatcgtat tcgcgtcgcg
     2761 tgagtctcag tcgatccggg tcagttcggc ctggatcgcc aaatccaggc cggaagcggg
     2821 gatttggcaa tctccttcga gcaatcctgg acataccgcg tctacagccg tccgagccaa
     2881 tcaaacggag tagagggagg aattcatgtc ttcaatcgcg ctcagagcgc gtgtcgttct
     2941 gcttgtttcg ctcgccctgg cgctggcggc ctgcggcggg actgcgcccg ccacaagcga
     3001 caccatgtcg gaggagccgc aagaagtcac catgactatg caagagttca agtttgaccc
     3061 caccgaaatt tcagccaggg tcggccggcc ggtcaaagtg acgctggtga acaagggcac
     3121 gctgctccac gacttcagca gcatggacgc gaaagtcgaa gtcgtgtcta tgcaggaagg
     3181 ggcagcacat gacatgggcg gtatggaaag tcaaatgacg atgcacatgg ccgtcgacgc
     3241 cagcacgacc gagacgctgg agttcaaacc gaccgaagca ggaacgtacg agttctactg
     3301 ttcggtcgaa ggacacaagg aagccggcat ggtcgggcag ttgatcgtca caccctaggc
     3361 acagggcacc cttgcgaagg ctttgcagcc ttcgcaaggg tccagggtcc aaaggaggtc
     3421 gaatgaaaga gtcaaccatt caagtcgatg gattgttgac cgccttatgc gcccgcaatg
     3481 tcgagaaggc gctcatgaag atgccgggcg tgcatcacgt ctcggcgaat taccttaaca
     3541 gcacggcgac cgtgcactac gacgagtcgc aggtatcgct cgcgcagctc caggcggcgg
     3601 tgtccgattg cggctacgtg tgcgcgggcg aggcgctgcc agagcacatg cagcatgcag
     3661 ggcacgccga acacgcggca gccatgccga tggacgctgg cgcgcatgcc gcgcatgcag
     3721 gacatgccat gcctgctgcc ccgcctgcgg cgcatgccgg tcacgtcgca gccggagtga
     3781 tggaagacca cgcggcgcac ggcggccggc cgggcatgac cgccggcgag atggaacgcg
     3841 acatgcgcaa tcgcttcttc gtgtcgctcg tcctgaccgt gccggtcttc ctcttctccc
     3901 acctggcgac gcagatcctc ggcctgcaca tccccctgcc cttcggcatc ggcgacaagg
     3961 tgtttgggtt catcctcacg acgccggtcg tgctgtacgg cgcgtggccg ttctacgtcg
     4021 gcgcgcgcaa cggcctgcgc cagggtgcgc tgaacatgag cgtcctggtc tcgttgagcg
     4081 tgctgaccgg ctacctcttc tccgtcgcgg cgacgttcct gttcgacggc gaggtgttct
     4141 acgaagcggc ggcgatgctc gtgacgttcg tgctgttcgg gcactggatg gagatgcgct
     4201 cgcggcgcgg caccagccag gccatccaga aactgctgca gctcgcgccg ccgaaagcga
     4261 ctgtcgtgcg cggcggcaaa gagatcgagc tgccgaccga gcagatcgtc gtcggcgacg
     4321 tcgtcatcgt gaagccgggc gacaagatcg cggtcgacgg cgtgctgatc gacggcgagt
     4381 cgagcgtcga cgagtcgatg atcacgggcg agagcctgcc ggccaagaag aaggccggcg
     4441 acccggtcat cggcgcgacg atcaacaaga ccggatcgtt caagttccgc gcgacgcgga
     4501 tcggcgcgga caccgcgctg gcgcagatcg tcaagatggt gcaggcggcg cagaactcga
     4561 aggccccggc gcagcggctg gccgatcgcg ccgcgcacta cctggtgttg ttcgccgtgt
     4621 tcgccgggct gggcacgttc ctgctctggt acttcgtcct cgcgccgcag ttcatgcccg
     4681 ccgatgcgga tcggctggtg ttctcgttga cgttcgcgat caccgtcgtc gtgatcacct
     4741 gtcccgatgc gctggggctg gcgaccccga cggcgatcag cgtcggcacg ggactgggcg
     4801 cgcagcacgg catcttgttc aaggacgcga cggcgctcga aggcgcggcc aagatcgacg
     4861 cggtgatctt cgataagacg ggcacgctga ccaagggcga gcc
//
LOCUS       DBNHXB010000133         2633 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2542138, whole genome shotgun sequence.
ACCESSION   DBNHXB010000133 DBNHXB010000000
VERSION     DBNHXB010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2542138"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="AC162G_05135"
     CDS             complement(<1..757)
                     /locus_tag="AC162G_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019584466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5075232.1"
                     /translation="MSDTHLTDVSFTNLHLPDALARGIADAGFERCTPIQAQTLPRAL
                     AGMDVAGPAQTGTGKTAAFLVAMYATLMRREVPVDRLLNAPRALIIAPTRELAVQIHH
                     DAEILGQYTGLSLGLAFGGVDYDKQRHQLAQGVDILIGTPGRLIDFYKQRVFDLRAIQ
                     VLILDEADRMFDLGFITDIRYIMRRLPAPDQRLNLLFSATLAQRVLELAFEHMNDPEL
                     VRIEPDKMTVDRVRQVIYYPSMDEKPRLLVGLLR"
     gene            1063..1389
                     /gene="trxA"
                     /locus_tag="AC162G_05140"
     CDS             1063..1389
                     /gene="trxA"
                     /locus_tag="AC162G_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HIR5075233.1"
                     /translation="MSQQIIHVTDSSFEQDVLKNTTPVLLDFWAEWCGPCKMIAPILD
                     QIATEYAGKVVIAKMNVDENPKTPMRFNVRGIPTLILFKNGQAEGQKIGAVRRADVAA
                     FLDSNL"
     gene            1563..>2633
                     /gene="rho"
                     /locus_tag="AC162G_05145"
     CDS             1563..>2633
                     /gene="rho"
                     /locus_tag="AC162G_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014869812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR5075234.1"
                     /translation="MNLSELKHKPIPQLLDVAAQMGLENLARSRKQDIIFSILKAHSR
                     NGESIYGDGVLEILQDGFGFLRSPEGSYLSGPDDIYISPSQIRRFNLRTGDTISGLIR
                     PPKDGERYFALLKVGEINFDSPDNARSKVLFENLTPLHPTRRFKLERGTGSTEDLTAR
                     MIDLIAPLGKGQRGLIVSPPKAGKTMLLQNIANSITGNHPEVYLIVLLIDERPEEVTE
                     MTRTVRGEVVSSTFDEPASRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTILATALIDTGSRMDDVIYEE
                     FKGTGNSEIHLDRRIAEKRVYPA"
BASE COUNT          546 a          815 c          830 g          442 t
ORIGIN      
        1 gtcgcagcag gccgacgagc agccgcggct tttcatccat cgacgggtag tagatgacct
       61 gccgcacgcg gtcgacggtc atcttgtcgg gttcgatccg gacgagctcc ggatcgttca
      121 tgtgctcgaa cgcgagttcg agcacgcgct gcgccagcgt cgcggagaac agcaggttca
      181 gccgctgatc gggcgccggc aggcggcgca tgatgtagcg gatgtcggtg atgaaaccga
      241 gatcgaacat gcgatcggct tcgtcgagga tgagcacctg gatcgcgcgc aggtcgaaca
      301 cacgctgctt gtagaaatcg atgagtcggc ctggcgtgcc gatgaggata tcgacgccct
      361 gcgcgagctg gtggcgctgc ttgtcgtagt ccacgccgcc aaaggccagc ccgagcgaga
      421 gcccggtgta ctggccgaga atctcggcgt cgtgatgaat ctgcacggcc agttcgcgcg
      481 tcggcgcgat gatgagcgcc cgcggtgcgt tcaacagccg gtcgaccggt acttcacggc
      541 gcatgagagt tgcatacatg gcgaccagga acgcggcagt cttgccggtg ccggtctggg
      601 ccggcccggc gacatccatg ccggcgagcg cgcggggcag ggtttgggcc tggatggggg
      661 tgcaacgctc gaatccggca tcggcgatgc cacgggctag cgcgtcaggc aggtggaggt
      721 tggtgaagga tacgtcggtc aggtgtgtgt cggacatcgc ggaagctggt gtatgcggcc
      781 ttgcaaatta ggaaaaagcg ggttcaaata acgcaatagc ggatcaatcc gctcccgcca
      841 gaatagttcg ccggccaatc gggccttagc ccggtatccc tctgtccggt gtttccggcg
      901 caacggaatt tcttcaaaag cagtcaacgg ccagccattc ccatagtttg ccctacgcga
      961 accccgccgt cacccgattc cgggacagac ccatcaactt ctcgcggata aaccacaaca
     1021 cgtgcttcaa ataaccctaa atgccagcgg aggtaatctc tggtgtccca gcagatcatc
     1081 cacgtcactg actcgagctt cgagcaggac gtgctcaaaa acacaacgcc cgtcctattg
     1141 gacttctggg ctgaatggtg cggcccatgc aagatgatcg caccgatact ggaccagatc
     1201 gcgacggagt acgcggggaa ggtcgtgatc gccaagatga acgtggacga gaaccccaaa
     1261 accccaatga ggttcaacgt ccgcggaatc ccgacactga ttcttttcaa gaacggtcag
     1321 gccgaaggcc agaaaatagg cgcggtccgc cgagcggacg tcgcagcttt cctggacagc
     1381 aacctgtgag aggctacctc ggcaaccagg ggcggaatcg ccccggtaag cgtggccgag
     1441 gtggtggcgg accggacggc aatcgtggtc cccggcagga cgaactgccg ccagacgatt
     1501 acatggacgg cgacgacctc gacgtcatca gcccggctga gctcgaagcc agccgcaagt
     1561 cgatgaattt gagcgagctc aagcacaagc cgatcccgca gttgctcgac gtcgcggccc
     1621 agatgggcct cgagaatctt gctcgctcgc gtaagcagga catcatcttc tcgatcctga
     1681 aggcgcattc gcgcaacggc gaaagcatct acggcgacgg cgtgctcgag atcctgcagg
     1741 acggcttcgg cttcctgcgg tctcccgagg gctcgtatct ctccggcccg gacgatatct
     1801 acatcagccc gagccagatc cggcgcttca acctgcgcac cggcgacacc atctcgggcc
     1861 tgattcgccc gccgaaggac ggcgaacgct acttcgccct gctgaaggtc ggcgagatca
     1921 acttcgactc gccggacaac gcacgcagca aggtgctctt cgaaaacctc acgccgctgc
     1981 acccgacgcg gcgcttcaag ctggagcgcg gcaccggcag caccgaagac ctcaccgcgc
     2041 gcatgatcga cctgatcgcg ccgctcggga agggccagcg cggcctcatc gtttcaccgc
     2101 ccaaagccgg caagacgatg ttgctgcaga acattgcgaa ttcgatcacg ggcaaccatc
     2161 ccgaggtgta cctcatcgtg ctgctcatcg acgagcggcc cgaagaagtc accgagatga
     2221 cgcgtaccgt gcgcggcgaa gtggtttcgt cgacgttcga cgaaccggcc agccgtcacg
     2281 tgcaggtggc cgagatggtc atcgagaagg cgaagcgcct cgtcgagcac aagaaggacg
     2341 tggtgatcct gctcgactcg atcacgcgcc tggcacgcgc ctacaacacc gtggtgccca
     2401 gctccggcaa ggtgctgacg ggcggtgtcg atgccaacgc gctgcagcgg ccgaagcgct
     2461 tcttcggtgc ggcgcgcaac atcgaggaag gcggcagcct gacgatcctc gccacggcgt
     2521 tgatcgacac tggttcgcgc atggatgacg tgatctacga agagttcaag ggcaccggca
     2581 acagcgaaat ccacctggat cgtcgcatcg ccgaaaagcg tgtctatccg gcg
//
LOCUS       DBNHXB010000134        13197 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_26908, whole genome shotgun sequence.
ACCESSION   DBNHXB010000134 DBNHXB010000000
VERSION     DBNHXB010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 13197)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13197)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13197
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_26908"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..702
                     /locus_tag="AC162G_05150"
     CDS             133..702
                     /locus_tag="AC162G_05150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5075235.1"
                     /translation="MPSLIIRPLTVADVPHLDRIDANLESDRFLDLEKAVDGLGASWR
                     LIERPLVPPFVSTDYGLHADERPALIERLRRNDGLYLVAEDDTRPAALLDVEVEHWRK
                     SANVWHLFVDRAYRRRGLGRQLMQRAFDWARASGLRGIVCETQTNNWAACNFYRKLGF
                     QLSGIDDHFYSNQDIALREVALFWWYELG"
     gene            743..2101
                     /locus_tag="AC162G_05155"
     CDS             743..2101
                     /locus_tag="AC162G_05155"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIR5075236.1"
                     /translation="MAEVTLYFPKGFKWGCATSPYQTEGDNTHSDWWVWEQGEGNIAG
                     GQKSGKACDWWGDGFLADLDWMARLNNNAHRMGVEWSRVEPDEGRYDDAAIDRYRFML
                     KSMRERGIEPMVTLHHFSNPIWVAERGGWESPDILPLFERYVDKVVASLKDLCEVWIT
                     VNEPTVYAALGYLQALSQDSVRHQTTFPPGKNDADLMLAVCENLMLGHAGAYHAIHRL
                     QPEARVGIAHAMQILDPLNPRSLLDRWALKQHDRFTNQSVLAAVFSGRILRPVGWRRV
                     RALRDTVDFIGLNYYARELVAFDRRSPQTLFGRRVQNPDGEISDKNYGEVYPRGLYRL
                     LRRLKRTGKPIYITENGLPDHDDDRRPAFLVNHLKQVWHAINDNVPVMGYYHWTLTDN
                     FEWAEGWNLRFGLVELDPDTQRRSLRRSGELYAEVCKANVLDTDMVRRYAPQLMETVF
                     KG"
     gene            2167..2253
                     /locus_tag="AC162G_05160"
     tRNA            2167..2253
                     /locus_tag="AC162G_05160"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2203..2205,aa:Ser,seq:gga)
     gene            complement(2333..2815)
                     /locus_tag="AC162G_05165"
     CDS             complement(2333..2815)
                     /locus_tag="AC162G_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5075237.1"
                     /translation="MTEPYVILKELDAVRAETLKKLNELTQEQLDWRPATSASGEEIW
                     SLGEIFMHLAIDEHYLREQIARPLLEGVKPPKGVSFLPPPPPYGTPKEVIRFWFERAR
                     TMTRRMIEGWPADANLELKHTSGLEPMNGVEWLKGYGGHETFHHRQIDALIAQLADRQ
                     "
     gene            complement(2863..3297)
                     /locus_tag="AC162G_05170"
     CDS             complement(2863..3297)
                     /locus_tag="AC162G_05170"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR5075238.1"
                     /translation="MLKVEKSISIAAKPERVWQAWTGEISKWWTRPYFNDAERATGLM
                     LEPHLGGRFIETWGDRGAGYLIGHVIEWLPPHRFAFTWAEKPWSGVSTVVWVTLKAEG
                     QATRVTLIHEGFDRLPDGAAQRSSYEAGWGDLLGKLKAYVEH"
     gene            complement(3284..3625)
                     /locus_tag="AC162G_05175"
     CDS             complement(3284..3625)
                     /locus_tag="AC162G_05175"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5075239.1"
                     /translation="MSAAVTPYKAIADRTRRLILDALREESLSAGAIAQRFPKMSRPA
                     VSKHLAILRRSRLVVMKKRGRERLYALNAAPLRDVSEWVHQYEVAWDGQLQSLKRYVE
                     SESKEEPDAES"
     gene            3739..4013
                     /gene="ffs"
                     /locus_tag="AC162G_05180"
     ncRNA           3739..4013
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC162G_05180"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            4100..5569
                     /locus_tag="AC162G_05185"
     CDS             4100..5569
                     /locus_tag="AC162G_05185"
                     /inference="COORDINATES: protein motif:HMM:NF019142.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HIR5075240.1"
                     /translation="MELSPLTRHPSRRRSTIAPKAVVAFAALVLAFGAMAAGYNLRLK
                     AVTLVVDGQPFPIRSNQNTVGGVLRDAGLTLYPEDRVTPALDDLVEEIDTIDIAHARP
                     LTILVDRQTLAVRTHAATLAELFTQYGIRINPQDALHVDGNPAGIGSGFAGAPTTSHT
                     VSIKRAVPLTITFDDGSSRTLLTTQATVGQALKEAGIEEYAADRLTPAANSRITGPTS
                     AHIERSVPVSVQVDGRAIRTRTHAERVGDLLAELGVPLQGQDYTQPPLDAPVQPDLSV
                     RVVRVAESFFIEQEPVAFETQILPNPDMEIDTQQLVQEGQSGVLQKRIRVRYEDGQEV
                     GRQLEDRVLVRAPQHKIIHYGTQIVIRTIDTPSGPREYWRRFRAYATSYSAATAGTPR
                     TAPWYGVTATGLKMRKGIVAVDPTVIPLRTELYVPSYGVGLAGDTGGGVIGRWVDLGY
                     DDNNLVVWHQWVDVYLLTPAPPADKIKYILPDWPTFPGR"
     gene            5614..6510
                     /gene="rsmA"
                     /locus_tag="AC162G_05190"
     CDS             5614..6510
                     /gene="rsmA"
                     /locus_tag="AC162G_05190"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR5075241.1"
                     /translation="MSSASEVRDLLARFNLRPRKSLGQNFLVDDAALDRIVSAADLTP
                     EDTVLEIGPGLGTLTRRLARAAGQVIAVELDQNLIPILRYTLAGYSNIELVHGDILKI
                     DPPSLVGESLHGPTDHPTTRLPDYSTTPSPHYKVVANLPYYITSAVIRHLLEAPAHPS
                     RLILTVQLEVAQRLIAGPGDMSLLAVGVQFYGAPAIVARIKAGSFYPAPKVDSAVVRI
                     EVHAQPPVGVRDVDHFFAVVRAGFGQKRKQLHNSLRIGLSLPAEVVEAALAKAGIDAK
                     RRAETLALEEWAALAQTLPDMK"
     gene            complement(6557..7243)
                     /locus_tag="AC162G_05195"
     CDS             complement(6557..7243)
                     /locus_tag="AC162G_05195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5075242.1"
                     /translation="MTWPHSSRSELPRRHTTPGFHHAVRLVARLVAMVATRVDAQGVE
                     RVPAQGAFVGVSNHLSSFDPLVVLAVMPVRPGTAFAAIEHRRDFLVGWALDRLGVIWI
                     DRDNPSRNALRIALNELAHGTAFGIAPEGTRSRSGGLLPGKTGAAYLATRAGVPILPV
                     AIWGTEKVAGNLRRLKRQTVHLRVGELIHLPQGRAGTEELEEYTDLIMHRIASMLPPE
                     YRGVYADRVK"
     gene            complement(7240..8229)
                     /locus_tag="AC162G_05200"
     CDS             complement(7240..8229)
                     /locus_tag="AC162G_05200"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HIR5075243.1"
                     /translation="MRRSAPWIALVALATGCGRPTDAPAPTPEPLGVTLPAPTPALRV
                     TRSVGEQPPLPTAIPAPTPTPVIHIVQPGETLIDIAQDYGVTLDALQAANGVLTPETL
                     QIGQRLIIPLGPGAPSTSGGGLLLPTPAPLPVSVQGAALYETPVGSLVVQGEILNTSG
                     VALENTQVRVALLNDSGEEVAAATAFTALEVIPMDGKSPFGVLFAPSDVPPGAASFVI
                     TPIRAEASTEPGSRYMPIVVTGQEGAVDGLQFKVSGSVANQGSATATGVNVVLTTYDD
                     TGRVTGFRQATVGDGTLPAGATAEFEIGLAPNSLQGLPPADYALAAQGQAAQP"
     gene            8529..13151
                     /locus_tag="AC162G_05205"
     CDS             8529..13151
                     /locus_tag="AC162G_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5075244.1"
                     /translation="MIIGIAALLFTALITIRLPPDPATPWLHVAIYLALFILARLSTI
                     SFHGTGFSLSHVILVSAFLTLDLTVALWIGFAGTLVEHAARLALYARRAAEPGTARDA
                     LGAAGAALGEATFSMGAAGPAFYTLGGETPITEIDLSQAVPILALFVVYSAADTLFQA
                     TPLGGARAASSRLFDPSTTLRAGFAQGRRGSRLSARDWLRQYLRTILVSNLLLLPLAL
                     IIASIYVRTDFPVFLVFVLFLVVVTLLIGVLGAAQWQLEKRLRERASVSAIGQALTSS
                     LDLPELLEAIHKQIGSVMDVRNFYVALYNEDYDELSFPLVYEDGQAVRYRSRMAGNGL
                     TEHVLHTRAPLLIPSDVAGAIRRLGLESIPGKEAHCWLGVPITTGERVLGVIAVQSHA
                     QSNAYDADDVDLLTTIAIQTAVAIENAQLYASTRRRAAELAILNSVSTVVGSSLNLDQ
                     VMEAVVGSVGPVVGCQKAAILLLQNGGLNFAPTASRGLGQHFLQKMPAMLRLGRGEKQ
                     AAGSERQPLVVNDARADPRFEQFRALIDSESIRSFADMPLHARDHVIGTLAVFYTEPH
                     RFTVADLDLLTTFANQAAVAVANASLYAQTDLALAQRIEELAALEEIGRELTSTLDFN
                     RVIERVLDAAVEFAGASRGLVALHDAGWPLAVPSAQGQGFRLGLVAVRGSAPGTFERS
                     EVESWLGRQEMIAAVIRDGTVQWIEDVRGAPALSGSPLPSAGDPSTSLRAGFAGQAYE
                     PNDPETRSALAVPIVRDGQPLGVIVLESTRRAAFNEASARFIGQLATQAAVAIHNAQL
                     HEQTQGKLREMSILFELSREVTAILDLPELGREIAGRLAQALGTTHCAIELMGHNSER
                     LERIAEHVAPEADAGDPSTALRAGLPPEVVGAIRSLDVEGLRQNRQPVILYAAEWPLP
                     LPHAHPLRAAQGQGFGLADVEILRAHNLCSLIGLPLIAGNQVIGRVLWMDGRQRAPFS
                     ADEIRFAETLANQASIALENARLFRERARRVNDLAQLYQASLALASSFELEEALNRIA
                     VVAREITDSDSVAVYLYDGQTDSITHGSQLVGSSRRADVSMVRPHGMTRRIIRTRQPM
                     LVHDTHLEREMNPRVLEAGIRSVIGMPIIRKGEVLGVVYVNSRAVGKYTADSVQLLQL
                     LANDASVAIENAGLFSRMAETRDRLAAILNSSRDGVLMFDVSGRIIVANPMIEQMWSM
                     RRSELEGQSLARLLEAPGLDLPALSGYSRGALRALLEQATRGVHVVWGKEVFTLPGAL
                     GARWIERTGAPVLDENGGLVGWMLVFRDVTEERELQVMREDLTNMIVHDLRSPLVAIL
                     DAYELIGESLPADVHTGLTGKALDVGQRSTRKLLDLVNSLLDMSRFEHGQVSVDTQFA
                     ALRPLTENALAQLAPMLADRGLVVHNDVPADMPLVKVDEDQITRVLINLIDNAVKFSP
                     QGGRIVITARPGSSHDGVPSQNVVCSVRDMGPGVPAEYRERIFERFVHLSDGLERRRG
                     TGLGLAFCKMAIEAHGGSIWIEEPPEGQGSQFSFTLPVADLQPIPP"
BASE COUNT         2278 a         4140 c         4353 g         2426 t
ORIGIN      
        1 cgggcgaata tcgcgccgtc ggctggaacc cggatctcag ccactacttc cgtctttaac
       61 gtgttgcgta gtgcgtgttt cgtcacgcaa cacggaatac gcaccacgga tccgccgtgt
      121 gcaacggtca gtatgccatc cttgatcatc cgcccgctca ccgtcgccga cgtcccgcac
      181 ctcgaccgga ttgacgccaa tcttgaatcg gatcgcttcc tcgacctgga aaaggcggtg
      241 gatggactgg gcgcctcgtg gcggctgatc gagcgcccgc tcgtgccgcc cttcgtctcg
      301 acggattacg gcctccacgc cgacgagcgc ccggcgttga tcgagcgcct gcgccggaac
      361 gacggcctgt atctggtggc ggaagacgac acacgcccgg cggcgcttct cgacgtcgag
      421 gtcgagcatt ggcgcaagtc ggccaacgtg tggcatctct tcgtcgaccg cgcatatcgg
      481 cgacggggac tgggcaggca actcatgcag cgcgcgttcg attgggcgcg cgcctccggg
      541 ttgcgcggca tcgtatgcga gacgcagacg aacaactggg ctgcgtgcaa cttctaccga
      601 aaactcggct tccagctgag cggcatcgac gatcacttct actccaatca agatattgct
      661 ctcagggaag tcgcgttgtt ctggtggtac gagttgggct gacgaatgta gaatgaagaa
      721 tgcagaatgt agaatgtaga aaatggccga agtcacactt tacttcccca aaggcttcaa
      781 atggggctgc gccacatcgc cctaccagac cgaaggcgac aatacccaca gcgactggtg
      841 ggtgtgggaa cagggcgagg gcaacatcgc gggcgggcaa aaatcgggta aagcctgcga
      901 ctggtgggga gacggcttcc tggcagacct ggactggatg gcgcgcctga acaacaacgc
      961 gcaccgcatg ggcgtcgagt ggagccgcgt cgagccggac gagggccggt acgacgacgc
     1021 ggcgatcgac cgctatcggt tcatgctcaa gtcgatgcgc gagcgcggca tcgagccgat
     1081 ggtgacgctg caccatttca gcaaccctat ctgggtggcc gagcggggcg gttgggagtc
     1141 gcccgacatc ctgccgcttt tcgagcgcta cgtggacaaa gtcgtcgcgt cgcttaaaga
     1201 tctgtgcgag gtgtggatca cggtcaatga gccgacggtg tacgctgcgc tcggctatct
     1261 ccaggctcta tcgcaggaca gtgtgcgcca ccagacgacg ttcccgcctg gcaagaacga
     1321 tgccgacttg atgttggccg tctgcgagaa cctgatgctc gggcacgctg gcgcatatca
     1381 cgccattcat cgtctgcagc ccgaagcgcg cgttggcatc gcgcacgcca tgcaaatact
     1441 cgatccgctc aacccgcgct cgctgctcga tcgatgggcg ctcaagcagc acgatcggtt
     1501 cacgaatcaa agcgtgctcg ccgcggtgtt ctcggggcgt atcctgcgcc cggttggatg
     1561 gcgccgcgtg cgcgctctgc gcgacacggt cgacttcatc gggctgaact actacgcgcg
     1621 cgagctcgtc gccttcgatc ggcgctcgcc gcagacgctg ttcggccgtc gtgtgcagaa
     1681 tcccgacggc gagatcagcg acaagaacta cggcgaggtg tatccgcgcg gattgtatcg
     1741 cctgctgcgc cgcctgaagc gcaccggcaa gccgatctat atcaccgaga acggcctgcc
     1801 cgatcacgac gacgaccggc gacccgcttt cctcgtcaac catctcaagc aggtgtggca
     1861 cgccatcaac gacaacgtgc ccgtgatggg ctattaccac tggacgctga ccgacaactt
     1921 cgaatgggcc gagggctgga atctgcgctt tgggttggtc gagctggacc ccgatacgca
     1981 gagacggtca ctgcggcgga gcggcgagtt gtacgccgag gtctgcaagg ctaatgttct
     2041 cgacaccgac atggtgcgcc gctatgcgcc tcaactgatg gagacggtgt tcaaaggatg
     2101 aaggatgacg ttcttcatct ttcatccttt ccccgtggta taatccgcct cgtagtcggc
     2161 ccatacggag aggtagcgta gtccggccta acgcgcgcgc ctggagagcg cgtgtgccga
     2221 aaggcaccgc gggttcaaat cccgccctct ccgcctttag cagatagcat atcgctgatg
     2281 gcagttggcc tatggcttcg agcagaagag gccataagcc atttgctatc agtcactggc
     2341 gatcagcgag ctgcgcgatg agcgcatcga tctgcctgtg gtggaacgtc tcgtgcccac
     2401 cgtacccctt cagccactcc acgccattca tcggctccag gccactggtg tgcttcaatt
     2461 cgagattcgc gtccgccggc cacccttcga tcatccggcg cgtcatcgtg cgagcgcgtt
     2521 cgaaccagaa ccggatcacc tccttcggcg tgccgtacgg cggcggtggt ggcaggaagg
     2581 acacgccctt cggcggtttg acgccctcca gcagcggccg cgcgatctgc tcgcgcaggt
     2641 aatgctcgtc gatggccagg tgcatgaaga tttcccccag cgaccaaatc tcctcacccg
     2701 aagccgacgt agcaggccgc cagtcaagtt gctcctgtgt cagctcgttt aatttcttca
     2761 aagtctcggc tcggacagca tccagttctt tcaggatgac atatggttca gtcacgttgt
     2821 cagtctcctc ccggcaaagg tttcagatgg ctgttccagc gtttaatgtt cgacgtaagc
     2881 tttgagcttg ccgagcagat ctccccaacc cgcctcatag ctggagcgct gggcagcacc
     2941 atccggcaat cgatcgaacc cttcgtggat gagggtcact cgcgtggcct gtccctctgc
     3001 tttgagcgtc acccacacca cggttgaaac tcccgaccag ggcttctccg cccacgtgaa
     3061 cgcaaagcgg tgcggcggga gccactcgat gacgtggccg atcaggtaac ccgcgccgcg
     3121 atccccccag gtctcaatga agcgcccgcc gaggtgtggt tctaacatga gacccgtcgc
     3181 gcgctcggca tcgttgaaat acggtcgcgt ccaccacttc gaaatctcgc cggtccacgc
     3241 ctgccacacc cgctctggct tcgcggcgat ggagatcgac ttttcaactt tcagcatcag
     3301 gttcctcctt ggattcggat tcgacgtagc gcttgagtga ttgcagctgc ccgtcccagg
     3361 cgacctcgta ctggtgcacc cactcggaga catcgcgcag cggcgcggcg ttgagcgcgt
     3421 atagccgctc acgcccgcgc tttttcatca cgacgagccg tgatcgccgc aagatggcga
     3481 ggtgcttcga aaccgccggc cggctcatct tcgggaagcg ctgggcaatc gccccggccg
     3541 acaggctttc ttctcgcaac gcatccagga taagtcggcg cgtgcgatcg gcaatggctt
     3601 tgtacggtgt gacggccgca ctcatacgta accatatggt tacacatttt cgcgtgtttg
     3661 tcaagcagct gactcgttgc ctgtccattg acaccatagg ttgaccgttc gtctcaaacg
     3721 tgatacgatt tcgcgcggtc gtgctagatg gggagctagt ggtgccctct cggcccagcc
     3781 gtgcccgcaa tccacttagc ggggtcgaat tccccctcga gggttgagcg gccgcagcct
     3841 gcccaggata agtggcgacg acgatcgggt ccggcgcggc agaagacttc gaaccccgcc
     3901 aggtccggaa ggaagcaacg gtaaggagac ccttctgggt gccgccgggg cgcctggtct
     3961 gagcaggctg tccgaagcgg tctgtggcgg cagattcgac agggggtgca cgaccacctc
     4021 acccccttcc ctgacccttc cccctgtccc aaggcaaagc gttgggacag ggggaagggg
     4081 atgaggttac gggaactccg tggaactcag tccgttgaca cgacatccat cgagacgccg
     4141 aagcaccatc gcgccgaagg ccgttgtcgc cttcgccgcg ttggtgctcg ctttcggcgc
     4201 gatggccgca ggttataacc tgcgcctcaa ggctgtcaca ctcgtcgtcg acggccagcc
     4261 gttcccgatc cggtcgaacc agaatacggt cggtggcgtg ctgcgcgacg ccggtctgac
     4321 gctgtatccg gaggatcggg tcacgcccgc gctcgacgat ctcgtggagg aaatcgacac
     4381 gatcgacatc gcccacgccc ggccgctgac gatcctcgtc gacaggcaga cgctggccgt
     4441 gcgcacgcac gcggcgacgc tggccgaact gttcactcaa tacggcattc ggatcaatcc
     4501 gcaagatgcg cttcacgtcg acgggaatcc ggctgggatc gggtcagggt tcgcgggcgc
     4561 gccgacgacg tcccacacgg tgtccatcaa gcgcgccgtt ccactcacca tcactttcga
     4621 cgatggctcg tcgcgcacgc tgctcacgac tcaagcgacg gtagggcagg cgctgaagga
     4681 agccggtatc gaggagtacg ccgccgatcg cctgacgcca gccgcgaata gccgcatcac
     4741 tgggcctact tcggcgcata tcgagcgctc ggttccagta tcggtgcagg tcgatgggcg
     4801 ggcgatccgc acgcgaacgc acgccgagcg tgtgggcgat cttctggcgg agttaggcgt
     4861 cccgctgcag ggtcaggact acacgcagcc cccgctcgac gcgccggtgc agcccgatct
     4921 gagcgtgcgc gtcgtccgcg tcgccgagtc cttcttcatc gagcaagagc cggtcgcctt
     4981 cgagacgcag atcctgccca atccggacat ggagatcgat acacagcagc tcgtgcaaga
     5041 gggccagagc ggtgtgctgc aaaagcgcat ccgcgtgcgc tacgaggacg gtcaggaagt
     5101 ggggcggcag cttgaggatc gcgtgcttgt tcgcgcgccg cagcacaaga tcatccatta
     5161 tggcacgcag atcgtcatcc gcacgatcga cacgccgagc gggccgcgcg agtactggcg
     5221 gcgctttcgc gcctatgcca cgtcgtacag cgcggcgacg gccggcacgc caagaaccgc
     5281 accgtggtat ggtgtcaccg cgaccggcct gaagatgcgc aagggcatcg tcgccgtcga
     5341 tccgacggtc attccgctgc gcaccgagct gtacgtgccg agctacggcg tcggcctggc
     5401 gggcgacaca ggagggggcg tcatcggccg ctgggtcgat ctcggctacg acgacaacaa
     5461 cctggtcgtc tggcatcagt gggtcgacgt gtatctgctc acgccagcgc cgcctgccga
     5521 caagatcaag tacatcttgc ccgactggcc caccttcccc ggccgatagg ccccttcccc
     5581 ttcactatcg ttctgagtca gggcaggctc tgcatgtctt ccgcgtccga agtccgcgat
     5641 ctcctcgccc gattcaacct gcgcccgcgc aagagcctgg ggcagaattt tctcgtcgat
     5701 gacgcggcgc tcgatcgcat cgtctccgcc gccgatctga ccccggaaga cacggtgctg
     5761 gagatcggtc ctggacttgg cacgctcaca cggcgcctgg cgcgggctgc cggccaggtg
     5821 atcgccgtcg agctcgatca aaacttgatc cccatcttgc gctacacgct ggccggctac
     5881 tccaacatcg aactggtcca cggcgatatc ctgaaaatcg atccaccctc cctcgtgggt
     5941 gaatcattgc acgggccgac cgaccatccg accacccgac tacccgacta ctcgactaca
     6001 ccatcccctc attacaaagt cgtggccaac cttccgtact atatcacgtc ggccgtcata
     6061 cggcatttgc tcgaagcgcc ggcgcacccg tcgcggctga tcctgactgt gcaactggaa
     6121 gtcgcgcagc gattgatcgc cgggccgggc gacatgagcc tgctcgcggt cggtgtccag
     6181 ttctacggcg cgccagccat cgtggcgcgc atcaaggccg gatcgttcta cccggcgccc
     6241 aaagtcgatt cggcggtcgt gcgcatcgag gtgcatgcgc agccgccggt cggggtgcgc
     6301 gatgtcgacc acttcttcgc cgtcgtgcgg gccggcttcg ggcaaaagcg caagcaattg
     6361 cacaactcgt tgaggatcgg attgtcgctg ccagccgagg tcgtcgaagc cgcgctggcg
     6421 aaggcgggga tcgatgcgaa acgccgggcg gaaacgctcg cactcgaaga gtgggctgcg
     6481 ctggcgcaga cactgcctga tatgaagtga ccagcacttc ggtccctccg cttcgggggt
     6541 ctgcgaaaag tgccggtcac ttcaccctgt cggcgtacac gccgcgatac tccggcggca
     6601 gcatcgacgc gatgcggtgc atgatcagat cggtatactc ttccagctcc tccgtgccag
     6661 cgcgcccttg cggcaggtgg atcagttcgc cgacacgtaa gtgcaccgtc tggcgcttca
     6721 ggcggcgcaa gttgcccgcg accttctcgg tgccccagat ggcgaccggg aggatcggca
     6781 cgccggcacg tgtggcaaga taagccgcgc ccgtcttgcc cggcagcaac ccgccgctcc
     6841 ggctgcgcgt tccctcaggc gctatgccaa atgccgttcc atgcgccagc tcgttcagcg
     6901 cgatgcgcag cgcgttgcgg ctcgggttgt cgcgatcgat ccagataaca cccaggcgat
     6961 ccaaagccca accgacgagg aaatcacggc ggtgctcgat cgctgcgaag gcagtgccgg
     7021 ggcgaaccgg catgacggcc aaaacaacta gcgggtcgaa cgagctgaga tgattggaaa
     7081 cgccgacgaa tgcaccctgc gccggcacgc gctccactcc ctgcgcgtcg acacgcgtgg
     7141 cgaccatggc gacgagtcgc gcgaccaggc ggacggcgtg gtggaagccc ggcgtcgtgt
     7201 gacggcgcgg cagctcagaa cgtgaggaat ggggccaggt catggctggg cggcctgccc
     7261 ttgtgcggcg agcgcgtaat cggcgggcgg taatccctgc aggctgttcg gcgcgaggcc
     7321 gatttcaaac tcggcggtcg cgcctgccgg aagcgtgcca tcgccgacag tggcctgccg
     7381 gaaaccggtg acgcggcctg tgtcgtcgta cgtcgtcagc accacgttga cgccggtcgc
     7441 tgtggcgctg ccctggttgg cgaccgaccc actgacctta aactggagtc cgtctacagc
     7501 accttcttga ccggtgacga cgatgggcat gtagcgactc cccggctcgg ttgacgcctc
     7561 ggcgcgaatg ggagtgatga cgaacgaggc agcgcctggc ggcacgtctg acggcgcaaa
     7621 taacacgccg aacggagact tgccgtccat cgggatcacc tccagcgccg tgaaagccgt
     7681 tgctgcggcg acctcttcac ctgagtcgtt caggagagcc actcgcacct gcgtgttctc
     7741 cagggcgacg ccggacgtat tgagtatttc accttgcacg acgaggcttc cgaccggagt
     7801 ttcgtacagc gctgcgccct gaacggagac gggcagcggc gcgggtgtgg gcagcaacag
     7861 tccgccgccg gaggtcgacg gcgcccctgg ccccagtgga atgatcagac gctgaccgat
     7921 ctgaagtgtc tccggcgtga gcacgccgtt cgcggcctga agcgcatcga gcgtgacccc
     7981 gtagtcctgc gcaatgtcga tcagcgtctc tcccggctgc acgatgtgga tgaccggggt
     8041 gggtgtaggg gccggtatgg cggtcggcag cgggggctgt tccccaacag agcgcgtcac
     8101 gcgtagcgcg ggcgtcggcg ctggcagcgt cactccgagc ggctcaggcg tcggcgcagg
     8161 cgcatcggtt ggccgtccgc atccggtcgc gagagcgacc agcgcaatcc acggcgctga
     8221 acgtcgcatg cacatctctt tacctgccaa tggatggcgc gattatacca gttctccgat
     8281 ggcctgtggc gaccggtgcc atcttgtcgc tgcttctgtt ctgtgctaca ctcattatgg
     8341 acggtattgg tgctgaactc gctcgactct gcggcgctgc cttcggtgag cacggcggcc
     8401 gcgggtactt ctcaggcaac gcgctcgttg gcgtcggaac tccacctctc cagagctgaa
     8461 cccaggtatc cggtatgagt gtgcaccgct tggcgtccac gcgtgattcg atcagcaggt
     8521 ggtcgccgtt gatcatcggg atcgcggcgc ttctgtttac tgcgttgatc acgatccggt
     8581 tgccgccgga tcccgcaaca ccctggttgc atgtcgccat ctacttggcg ctattcatcc
     8641 tggcgcgact gtcgaccatc tcctttcacg gcacggggtt cagcctcagc cacgtcatcc
     8701 tggtatccgc ctttctcacg ttggatctga ccgtggcgct gtggattggc ttcgcgggca
     8761 cactcgtgga gcacgccgca cgcctggcgt tatacgcccg gagagccgca gagccgggaa
     8821 cggcgcggga tgcactcggc gcagccgggg ccgcgctggg cgaggcgact tttagcatgg
     8881 gcgccgccgg tccggccttt tataccctgg gcggagagac cccgatcacc gagatagact
     8941 tgagccaggc tgtgccgatc ctggctctgt ttgtcgtcta ttccgccgcc gatacattgt
     9001 tccaggcgac cccgctgggc ggggcgcgag cagcttcctc gcgcctcttc gacccttcga
     9061 ctacgctcag agcgggcttc gctcagggca ggcgtggatc acgcctgtcg gcgcgcgact
     9121 ggctgcgcca atacctacgc accatcctgg tcagtaatct gcttctgctg ccgctggcgc
     9181 tcatcatcgc ctcgatctat gtgcgcaccg acttcccggt gttcctggtc ttcgtgttat
     9241 tcctggtggt ggtcacgctg ctgatcggcg tgttgggcgc ggcgcagtgg caattggaga
     9301 agcgcctgcg cgagcgcgcc tctgtcagcg ccatcggtca agccctcacc agcagcctgg
     9361 acctgccgga gctgctggag gcgatccata agcagatcgg ctccgtgatg gacgtgcgca
     9421 acttctacgt ggcgctgtac aacgaggatt acgacgagct gtcttttccc ctggtctacg
     9481 aggatggcca ggcggtgcgt taccgcagtc gcatggcggg caacggcctg accgagcacg
     9541 tgctgcacac gcgggcgccc ctgctcatcc catctgatgt ggccggcgcg attcggcgcc
     9601 tgggcctgga atcgattccg ggcaaagaag cacactgctg gctcggggtg ccgatcacga
     9661 ccggcgaacg ggtgctgggg gtgatcgccg tgcaaagcca cgcacagtcg aatgcgtatg
     9721 acgcggacga cgtcgatctg ctgacgacga tcgcgatcca aactgcggtg gccatcgaga
     9781 acgcccagtt gtacgcttcc acccggcggc gcgcggcgga gctggccatt ctcaactcgg
     9841 tgtcgacggt ggtgggatcg tctctgaacc tcgaccaggt catggaagct gtggtgggtt
     9901 ccgttggtcc ggtggtcggc tgccagaagg cggcgatcct tttgctacag aacggcggtc
     9961 tcaactttgc gccgacggcc tcgcgcgggc tgggccagca cttcctccag aagatgccgg
    10021 ccatgctgcg ccttgggcgc ggggagaaac aggcggctgg cagcgagcgc cagcccctgg
    10081 tggtgaatga tgctcgggca gatccgcgct tcgagcagtt cagggcgctg atcgactctg
    10141 aaagcattcg ctcattcgcg gatatgccgc tgcacgcgcg cgaccacgtg atcggcacgc
    10201 tggctgtgtt ttacactgaa ccgcaccgct tcacggttgc cgatctcgac ctgctgacga
    10261 cgtttgcgaa tcaggcggcg gtggcggtgg ccaacgcgtc gttgtacgcg cagacggacc
    10321 tggcgctggc acagcgtatc gaggagctgg cggcgctgga ggagatcggc cgcgagctga
    10381 cgagcacgct ggatttcaat cgcgtgatcg agcgggtgct ggatgcggcg gtcgagttcg
    10441 cgggtgcatc gcgcggtctg gtggcgctgc acgacgcggg gtggcccctt gccgttccct
    10501 cggctcaggg tcagggcttc cgcctgggtc tggtggcggt gcgcggttcc gcgccgggca
    10561 cgttcgagag gtccgaggtg gagagctggc ttgggcggca ggagatgatc gcggccgtga
    10621 ttcgcgatgg aacggtgcag tggatcgaag acgtgcgcgg cgcgccagcc ctttcagggt
    10681 ccccactccc ttcggccggg gacccttcga cttcgctcag ggcaggcttc gcagggcaag
    10741 cgtacgagcc gaacgatcca gagacgcgct cggcgctggc cgttccgatc gttcgcgacg
    10801 gccaaccgct gggggtgatc gtgctggaga gcacacgccg cgcggcattc aacgaggctt
    10861 cggccaggtt catcggacag ttggcgacgc aggccgccgt ggcgattcac aatgcgcagt
    10921 tgcacgagca gacacagggg aagctgcgtg agatgtcgat cctgtttgag ctgagccgag
    10981 aggtgacggc gatcttggat ctgcccgagc tgggtcggga gatcgcgggg cggctggccc
    11041 aggcgctagg cacgacacac tgcgcgatcg agttgatggg gcacaactcg gagcggctgg
    11101 agcggatcgc ggagcacgta gcgcctgagg ctgacgccgg tgacccttcg actgcgctca
    11161 gggcaggcct gcctccggaa gtcgtcggcg cgatacgctc gctcgacgtg gagggattgc
    11221 gccagaatcg acagccggtt attctgtatg cggccgagtg gccccttccc cttccccacg
    11281 cccatcccct tcgcgctgct cagggtcagg gcttcggcct ggcggatgtg gaaattctgc
    11341 gtgcgcacaa tctgtgctcg ttgatcggcc tgccattgat cgcggggaac caggtcatcg
    11401 ggcgtgtgct gtggatggac ggccggcagc gggcgccgtt cagcgcagat gagatccgct
    11461 ttgcggagac gttagccaat caggcgagca ttgcgctgga aaacgcgcgg ctgttccgcg
    11521 agcgggcgcg gcgggtgaac gacctggcgc agttatacca ggcgagcctg gcgctggcct
    11581 ccagcttcga attggaggag gcgctcaacc ggatcgcggt ggtggcgcgc gagatcacgg
    11641 actcggattc ggtggcggtg tacctgtacg atggccagac ggacagcatc actcacggca
    11701 gccaactggt cggatccagc cgccgtgccg atgtctcaat ggttcggccg catgggatga
    11761 cgcggcgtat cattcgaacg cggcagccga tgctggtgca cgacacgcac cttgagcgcg
    11821 agatgaatcc gcgcgtgctg gaggcaggca tccgctcggt gatcggcatg ccgatcatcc
    11881 gcaagggcga ggtgttgggg gtggtgtacg tgaacagccg cgcggtcggg aagtacacgg
    11941 cggacagcgt gcaactgctg cagctgctgg caaacgacgc ctcggttgcg atcgagaatg
    12001 cggggttgtt cagccgcatg gcggagacac gcgatcggct ggcagcgatt ctgaactcct
    12061 cgcgcgacgg ggtgctgatg ttcgacgtgt ccggccggat catcgtggcg aatccgatga
    12121 tagagcagat gtggagcatg cgccgctctg agctggaggg ccagtcgctg gcgcgcttgc
    12181 tggaggcgcc gggactcgat ttgccggcgc tctcgggata ttcgcgcggg gcactgcgcg
    12241 cgctgctgga acaggcgacg aggggcgttc acgtggtgtg gggcaaggag gtgttcacgc
    12301 tgcccggggc cttgggcgcg cgctggatcg agcgcacggg ggcgccggtg ctggacgaaa
    12361 acggcgggct ggtaggctgg atgctggtgt tccgcgacgt gacggaggag cgcgagctgc
    12421 aggtgatgcg cgaagacctg acgaatatga tcgtgcacga tctgcgcagc ccgctggtgg
    12481 ctattctcga cgcgtacgag ctgatcgggg agtcgctgcc ggccgacgtg cacacggggc
    12541 tgacgggcaa ggcgctggac gtgggccagc gcagcacacg caagctgctg gacctggtca
    12601 attcgctgct tgacatgagc cgcttcgagc acggccaggt gagcgtggac acgcagttcg
    12661 cggcgctgcg cccgctgacg gagaacgcgc tggcgcagct cgcgccgatg ctcgcggatc
    12721 gcggcctggt cgtccacaac gacgtgccgg ccgatatgcc cctggtgaag gtggacgagg
    12781 atcagatcac gcgtgtgctg atcaatttga tcgacaacgc ggtgaagttc agtccgcagg
    12841 gcgggcgcat cgtgattacg gcgcggccgg gatcgagtca cgacggggtt ccgtcgcaaa
    12901 acgtggtgtg cagcgtgcgg gacatggggc ctggagttcc ggccgaatat cgcgagcgca
    12961 tattcgagcg gttcgtgcac ctgagcgacg ggctggagcg gaggcggggc acggggctgg
    13021 ggctggcgtt ctgtaagatg gcgatcgagg cgcacggcgg atcgatttgg atcgaggagc
    13081 cgcccgaggg ccagggcagc cagttcagtt tcacgctgcc agtggccgat ctccagccga
    13141 ttccgccgtg aaaactcgac cacggattgg ggcacggatg cacggattgg ggcaccc
//
LOCUS       DBNHXB010000135         5244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1201118, whole genome shotgun sequence.
ACCESSION   DBNHXB010000135 DBNHXB010000000
VERSION     DBNHXB010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1201118"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="AC162G_05210"
     CDS             <1..1090
                     /locus_tag="AC162G_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HIR5075245.1"
                     /translation="FATSIRSDAILREAHAAMADFLGAASPDEIVFGPNMTTLTFGVS
                     RAIGRTLEPGDEVVVTRLDHDANIAPWLALGERGVVVRWVDITPADCTLDIGSLEAAI
                     GERTKVVAVGYASNAVGTINDVKTIVEMAHAVGSLVFVDAVQYAPHGPIDVTDLDCDL
                     LACSAYKFFGPHVGALYGKYDLLDRLPAYKVRPAGDRPPDKFETGTQNHEGIAGTLGA
                     LRYFVWLGEQVISDQLAVNSASRRAKLVTAMMAIKENEKELSRALIEALSSIDGVKVW
                     GITDLNQLDRRVPTVSFTMEGRSPRAIAEYLAGHDIYVWDGNYYALAIMERLGLEESG
                     GMVRVGAAHYNTLDEVARLIRVLKAMPR"
     gene            complement(1146..2375)
                     /locus_tag="AC162G_05215"
     CDS             complement(1146..2375)
                     /locus_tag="AC162G_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR5075246.1"
                     /translation="MYFELWLPGGQAIALRGLDVKHILFCSIILLTVAACQTPQPSPA
                     PAMTALAPVSTAITPPVISATTAPTAEPTAVPSAAPATAPAAEPTAAPTESTATPKPT
                     QAPAASDVYVTAEGGLNLRAEASSSATLLRLLPFGTRLTALGQPTAPDATGIAWQNVQ
                     TDDGQSGWAAAQYLTNASPEAAAAPSPTPETPGSVPGFTPGYVYVASVGGLNMRAAAS
                     VSSSVIAMLADGQRLQTNGLSSGPDSAGITWLNVKTEDNIEGWVAAEFVREQVPSVAP
                     ATPPANVSDAVAEILRRTNDLRAQHGLNPYAFDEVLAQLALAHSQYMAQNGITHTGAG
                     GLSAKQRIANAGYGGRPTENIYGGQATIDQAWEYWATDPPHVDNLLNPFNTLVGIGVY
                     QTGLLTYYTMDFAKPPE"
     gene            2641..2850
                     /locus_tag="AC162G_05220"
     CDS             2641..2850
                     /locus_tag="AC162G_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR5075247.1"
                     /translation="MNERTQGTVKWFNATKGYGFISREGGEDVFVHHSAIQAEGYRSL
                     NEGQRVEFSVERGPKGLQASQVVIL"
     gene            3009..3512
                     /locus_tag="AC162G_05225"
     CDS             3009..3512
                     /locus_tag="AC162G_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075248.1"
                     /translation="MILAETVTQTWQRMTAMPDDQARQLVQQMNVEQPVILAYLLAMS
                     DQPPFDEHEGQLSFYVGLVIWQVMKQSPKRLRKVAQTQLKQAEQANEEFLDLLATDTE
                     GDFFSATKKMLETYPEPEVLGYILEVLLDEVDYASDDPPIREENRGPAFLHLKIILDA
                     FISSIDR"
     gene            complement(3562..3951)
                     /locus_tag="AC162G_05230"
     CDS             complement(3562..3951)
                     /locus_tag="AC162G_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075249.1"
                     /translation="MDRSTWPFGCGAACARQVMMPMLNSSSPVVRDEAPPALDVDLSL
                     FREDFRNEARAYLSAMWTFADAANLAAVEGAARAAMMGYSDIGELAAHAEHLLLDMRD
                     GKIGLDPIHLDTLRDLAQRVGQIVEVL"
     gene            complement(3897..4421)
                     /locus_tag="AC162G_05235"
     CDS             complement(3897..4421)
                     /locus_tag="AC162G_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024139.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5075250.1"
                     /translation="MAELLNTRDVARMYRVTSATVLNWIHAGKLKAYTTPGGHYRIAR
                     EDLEAFSHDYGYSPAAGIPTFGLRLLFVGSDAPLFERLRDAVRVQWPAAQVEQARTEF
                     EIGWWMARLRPTHLVVHFDLMPPALIELCKQLAGDAERELCLEALPGSPEANWGEWID
                     QLGPSAAAQRAPGR"
     gene            4677..5045
                     /locus_tag="AC162G_05240"
     CDS             4677..5045
                     /locus_tag="AC162G_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR5075251.1"
                     /translation="MIHTEHAPKAIGPYSQAIRSGGFVYTAGQVGFDPVSGQIVEGGI
                     EAQTRQALHNIKAILKAAGTSLDRVVKTTVFLADLSQFSAMNAVYAEFFPSNPPARST
                     FQVAALPRNALIEIEAIAEG"
BASE COUNT         1031 a         1543 c         1692 g          978 t
ORIGIN      
        1 atttgccacc agcatccgct ctgacgcgat cctgcgtgag gcgcacgcgg cgatggccga
       61 cttcctcggc gcggcctcgc ccgacgagat cgtcttcggc ccgaacatga cgacgctgac
      121 gttcggcgtc agccgcgcga tcggccgcac gctagagccg ggcgacgagg tcgtcgtgac
      181 gcgcctcgat cacgacgcga acatcgcgcc gtggctggca ttgggggagc gcggtgtggt
      241 cgtgagatgg gtggacatca cccccgccga ttgcacgctc gacataggtt cactcgaagc
      301 ggcgatcggc gaacggacga aggtggtggc cgtcgggtat gcttcaaatg cagtcggcac
      361 gattaacgac gtgaagacca tcgtcgagat ggcgcacgcg gtagggtcgc tggtcttcgt
      421 cgacgcggtg cagtacgcgc cgcacggccc gatcgacgtg accgatctcg actgcgacct
      481 gctggcttgc tcggcgtaca agttcttcgg gccgcacgtc ggcgcgctct atggcaagta
      541 cgacctgctc gatcggctgc cggcttacaa ggtgcgacct gcaggtgacc ggccgcccga
      601 caagttcgag acaggcacgc agaaccacga aggtatcgcg ggcacgctgg gggcgttgag
      661 atattttgtg tggctgggag agcaagttat cagtgatcag ttggcagtga acagcgcgag
      721 ccggcgggcg aaactggtca cggcgatgat ggcgatcaag gaaaacgaga aggagctgag
      781 ccgcgcgctg attgaagcgc tgtcgtcgat cgatggggtg aaggtgtggg gcatcaccga
      841 tctgaatcag ttggatcggc gcgtgccgac ggtgtccttc acgatggaag gcaggtcgcc
      901 gcgcgccatt gcggagtatc tggccgggca cgatatctac gtgtgggacg gcaactacta
      961 cgcgctggcg atcatggagc gattagggct tgaggaatca ggcggcatgg tgcgcgtcgg
     1021 tgcggcgcac tacaatacgc tggacgaagt cgcccggtta atccgagtgt tgaaggccat
     1081 gcccagataa agagttccga agtcttgcaa ggtgaccggc acgtcaaaag tgccggtcac
     1141 cttgtctatt caggcggctt agcgaagtcc atcgtgtaat acgtcaacag gccggtctgg
     1201 tacacgccaa tgccgacgag ggtgttgaac gggttgagca agttgtcgac gtggggcgga
     1261 tcggtggccc agtactccca ggcctggtcg atcgtggcct ggccgccgta gatgttttcg
     1321 gttggccgcc cgccgtatcc ggcattggcg attcgctgct tggcactcag cccaccggcc
     1381 cccgtgtgcg tgatgccgtt ctgcgccatg tactggctgt gcgccagcgc cagttgtgct
     1441 aggacctcgt caaaagcgta cggattcagg ccgtgctgcg cgcgcaggtc gttcgtacgg
     1501 cgcaggattt cagcgacggc gtcagacacg ttcgccggcg gcgtggccgg cgcaaccgac
     1561 ggcacctgtt ccctcacaaa ctccgccgcc acccaaccct caatattgtc ttccgtcttc
     1621 acgttcagcc aggtgatgcc cgcgctgtcc ggcccggagg acagcccgtt ggtctgaagc
     1681 cgctggccgt ccgccagcat cgcgatgacg gacgacgata cgctcgccgc cgcgcgcatg
     1741 ttcaaaccgc cgaccgaggc gacgtacacg tagccaggcg tgaagccggg cacggagccg
     1801 ggtgtctccg gcgtgggtga tggcgcagcc gccgcttccg gcgatgcgtt cgtcaggtat
     1861 tgggcggccg cccagcccga ctgcccgtcg tcggtctgca cgttctgcca ggcgataccg
     1921 gtggcatccg gcgcagtggg ctggccgagc gcggtcaggc gcgtgccgaa gggcagcagc
     1981 cggaggagcg tagccgatga gcttgcctcg gcgcgcaggt tgagaccgcc ctcggcggtc
     2041 acgtacacgt cgctggcagc cggggcctgt gtcggtttgg gcgtcgccgt cgattcagtt
     2101 ggcgctgccg ttggttcggc cgccggagcg gtcgccggcg ctgcggaggg aaccgccgtc
     2161 ggctcggccg tgggcgcggt cgtagcactg atgacaggcg gcgttatcgc ggtgctcacg
     2221 ggcgcgagcg cagtcatggc cggcgcaggc gagggctgcg gcgtttgaca ggccgccaca
     2281 gtcagcagaa tgattgagca aaacaagatg tgcttcacgt cgagtcctct caaggcgatg
     2341 gcctgacccc ccggcagcca taactcgaag tacaatacta tatcaccgat tgcatcgaga
     2401 accaaacggc ccggcaaaac atgccagcgg cattgccaca cgacggcctt gtgaataatc
     2461 accagtcaac agagtttgac gaaaacgagc ttatgtggta aactttttgt tgtaggatga
     2521 agaaagttcc tgttcggttc taagatgtcc gccaggcttt cgtcttggtg ggcatttttc
     2581 ttttccggcg gcgagcctct ttgacctaac aaatccaatt ctcagaagga gaaacaagct
     2641 gtgaacgaac gaacgcaggg caccgtcaag tggttcaacg ccaccaaggg gtacggtttt
     2701 atctcgcgtg aaggcggcga ggacgtgttt gtccaccact ccgcgattca ggccgaaggc
     2761 tatcgctcgc tgaacgaagg ccagcgggtc gagttcagcg tcgagcgcgg ccccaaggga
     2821 ctgcaggcca gccaagtcgt tatcctgtaa tcccgaaaga atctttcgca gtaacttccc
     2881 ccgttacacc gcgcctgccg gtgagtgcag gtgttgcgag cgcagcgaag caatctctcg
     2941 cgggcggagc tgagattgct ttgttgcaat tcttcgactc cgctgtgctc cgctcaggaa
     3001 cgctcctcat gatcttagct gaaaccgtta ctcaaacttg gcagcgcatg accgccatgc
     3061 ccgacgacca agctcgacag cttgttcagc agatgaacgt ggagcaaccc gttatcctcg
     3121 cttacttgct ggcgatgagc gatcaaccgc ctttcgacga acatgaaggc cagctctctt
     3181 tctacgtggg cctggtgatc tggcaggtta tgaagcagag tccgaaacgc ctccgcaaag
     3241 tagcccagac ccagctcaag caggccgaac aggcgaacga agaattcctg gacctgctgg
     3301 ctactgatac cgaaggcgac tttttcagtg cgacgaagaa gatgctggag acttatcccg
     3361 agcctgaagt gctcggctat atcctggaag tgctcctgga tgaagtggac tacgcttccg
     3421 atgacccgcc tattcgcgaa gagaatcgag gaccggcgtt tcttcacctg aagatcatac
     3481 tggatgcgtt catcagcagt attgaccgat agcctgttgt aaactccagt tgtaaagtaa
     3541 gcgctagcgc gctcaatccg attacagaac ctcaacgatc tgcccgacgc gctgcgcgag
     3601 gtcgcgcagc gtatccagat ggatcggatc gaggccgatc ttcccgtcgc gcatgtccag
     3661 cagcaggtgc tcggcgtgcg cggcaagctc cccaatgtcg gaataaccca tcatcgccgc
     3721 gcgggcagcg ccctcgacgg cagccaggtt cgccgcgtcg gcgaacgtcc acatggccga
     3781 aaggtacgcg cgcgcttcgt tccggaagtc ttcgcggaac agcgagaggt ccacgtcgag
     3841 cgccggcggc gcctcgtcgc gcaccaccgg cgacgaggaa ttcagcatcg gcatcatcac
     3901 ctgccgggcg cacgctgcgc cgcagccgaa gggccaagtt gatcgatcca ttcgccccag
     3961 tttgcttccg gcgacccggg gagcgcctcc aggcatagct cgcgctcggc atcgccggct
     4021 aactgcttgc agagctcgat cagcgcaggc ggcatcagat cgaaatgcac gacgagatgc
     4081 gtcgggcgca ggcgcgccat ccaccaaccg atctcgaact cggtgcgcgc ttgctcgacc
     4141 tgcgccgccg gccattgaac gcgcaccgca tcgcgcagcc gctcgaaaag aggcgcatcc
     4201 gagccgacga acagcagtcg aagaccgaag gtcgggatgc ccgctgcagg cgaatatccg
     4261 tagtcatgcg agaaggcctc cagatcttcc cgcgcgattc ggtaatggcc gcccggcgtg
     4321 gtgtacgcct tgagctttcc ggcgtgaatc cagttcagca cggtggcgct cgtgacgcga
     4381 tacatgcggg cgacgtcccg cgtattgagc agttcagcca tagagcgtgt ctccaaaatc
     4441 atctggtatc gatccgaaca ggatcgatgc ctcgattttc gcgcttttgg ggatttgtgc
     4501 agtttggggt gcgttgggaa cgtgttggcg cgtgcgtgtt gacagtcctc gtatgccgca
     4561 ttagaatcgg cggcacctta acgcggaggc gagccatggc gactcgtaaa cctgcttcga
     4621 aaaagcctgc ctcgcggcgg ccaaaaaagc ccgctggcaa aaaggaagtc cgaagcgtga
     4681 ttcacaccga gcatgcgccc aaggccatcg ggccgtactc gcaggctata cgttcgggtg
     4741 ggttcgttta cacggccggc caggtggggt ttgatccggt cagcggccag atcgtcgagg
     4801 gaggcatcga agcgcaaacg cgccaggcgc tccataacat caaagctatt ctcaaggcgg
     4861 caggcacgtc gctcgaccgc gtggtcaaaa cgaccgtgtt cctggccgac ctgagccagt
     4921 tctcggcgat gaacgccgtg tacgccgagt tctttccatc gaacccgccg gcgcgcagca
     4981 cgttccaggt ggcggccctg ccgcgaaacg cgctgatcga gatcgaggcg attgcggaag
     5041 gctgagattg gagattggag gttggaggtc ggagattgga gattggaggt tggagaaaag
     5101 gggatcgcga ttgcgatagg agataggcga tacgcgatag gcgataagcg ataggcgata
     5161 tgccaaagga aacgattctc ctcgtggacg acgaagcgaa tatcgtcgaa ctggcgcggc
     5221 tgtatctggc gcaggaaggc tacc
//
LOCUS       DBNHXB010000136         6789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1537114, whole genome shotgun sequence.
ACCESSION   DBNHXB010000136 DBNHXB010000000
VERSION     DBNHXB010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1537114"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="AC162G_05245"
     CDS             <1..267
                     /locus_tag="AC162G_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR5075252.1"
                     /translation="KWLEWSLNPKLQGDLAAWFGSVPVVLAACTGNALLTDAGCETNG
                     LGNFDTIRFWKTPTEDCGDGPGGMQGCVPYYRWVTDYLAVIGGR"
     gene            426..1454
                     /locus_tag="AC162G_05250"
     CDS             426..1454
                     /locus_tag="AC162G_05250"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012628999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5075253.1"
                     /translation="MPAVRLSGVSRHFGEVKALDNIHLDIVDGEFFSLLGPSGSGKTT
                     CLRLIAGFDQPTAGRIELHGEDVTDLPPYERSVNTVFQDYALFPHMNVADNVGYGLMI
                     NKAPRLERMKRVSEMLDLVQLPGMEQRKPSQLSGGQRQRVALARALIMHPRVLLLDEP
                     LGALDLKLRQKMQIELKAIQQRVGITFLYVTHDQEEALTMSDRLAVFNHGHIEQIGTP
                     AEVYEHPATPFVAGFVGVSNLIGGATAQAIFGTPDTFSIRPEKINIQAPGVSVPDGYC
                     SVSGRIRDVVYLGMYTRYLVEIDGGADLTVVRQNLDETSMDVLANRGRPVLLTWQREH
                     NRPIAALS"
     gene            1459..2406
                     /locus_tag="AC162G_05255"
     CDS             1459..2406
                     /locus_tag="AC162G_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007700690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5075254.1"
                     /translation="MTTASMTPAVSHPSAERRLSTFLYRRPVLSLLLLLIPPLLWLGI
                     LYIGSLLALLIQSFYRLDGFTGRVVREFGLSTYGDLLTQAHFDIMARTALMAAAVTIA
                     AAFVAFPIAYYMARYAGRRTRAFLYLAVLLPLWSSYLVRVYAWKFILAKEGVFHWFIS
                     QIGLQGLLNAVLDFPLIGGESLSVSPLGLFLVFLYVWLPYMILPVAAALERVPRSLLE
                     ASGDLGARPEATFQHVTLPLAFPGVVAGSIFTFSLTLGDFIIPTIIGDSSPFIGLAVY
                     QYQGTAGNVPLAAAFTIVPMVIMAAYLIIAKRLGAFDAL"
     gene            2396..3211
                     /locus_tag="AC162G_05260"
     CDS             2396..3211
                     /locus_tag="AC162G_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5075255.1"
                     /translation="MRSNRGRILAKPSLGLRLATIAVLLFLHFPLLIIILYAFNTQES
                     AFTFPPPGLTTEWFRVALTERPDLWSALWLSVGVALVATLVAIVLGTLIAVAISRSKF
                     FGRETISFIVVLPIALPGIVTGIALRSAIGLMEIPFTFWTIVIGHATFCVVVVYNNVV
                     ARLRRTGRSQMEASMDLGASSFQTFRYIVLPNIATALLAGAMLAFALSFDEIIVTTFT
                     AGQQTTLPIWIFSQLTRPRSQPVTNVAAVMVVAITFIPILLAHRLTQDTEASL"
     gene            3327..3887
                     /locus_tag="AC162G_05265"
     CDS             3327..3887
                     /locus_tag="AC162G_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015176715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR5075256.1"
                     /translation="MAVQLQRKLFTIDEYQQMIQAGVFHEDDRLELIEGEIAEMSPIS
                     PKHAGCVNRLADLFYDRLRRRAIIAVQNPVRVNQRSEPQPDVALLRPRPTYYGNLHPA
                     PEDVLLIVEVSDTSADYDRDVKMPMYAKAGIPEAWLVSLADGWMEVYREPSPVGYKSI
                     RRVLPGETVSPLAFPDVSLDVSEITE"
     gene            3907..3980
                     /locus_tag="AC162G_05270"
     tRNA            3907..3980
                     /locus_tag="AC162G_05270"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3941..3943,aa:Pro,seq:tgg)
     gene            complement(4033..6531)
                     /locus_tag="AC162G_05275"
     CDS             complement(4033..6531)
                     /locus_tag="AC162G_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015171315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR5075257.1"
                     /translation="MTLDVTALPLRGLSETEAQARRAKGQGNRVPPATGRTYVQIVRE
                     NVFTFINNVLFLLALALVLVGRPLDAILSVGVIATNIAVSVVQEIRAKRTLDRIALLT
                     RPQARIIRDGQERPIAPEELVVGDVLKVSAGDQIVLDGAVIDGRMSVDESQLTGESNL
                     IFKQPGDPVYSGSFCMSGSAVYVAEKVGEASLANQITSGARAFRRVLTPLQRQIHLVI
                     RVMLLIIIYIEALLVVNAVVKLVPAAESVTQATMIASLVPNGLFVSIAITYALAAVRI
                     IRFGALVQQSNAVESLSSVDVLCLDKTGTLTANRLQVSQLHAIGVGEQELQSVLGAMA
                     ASAAARNKTGEAIAAAFPERPRRTLAEVPFSSARKWSAVALEDDRRGVFAMGAPEMLQ
                     PYLGADGEAPSAVWQQISAHAGAWTSQGLRVLLVAHHPDPIRLKDGGDASCLPNGMTP
                     LGLVSLSDELRPEAREALAAFSAIGVEPKIISGDDPETVAALARQAGLSPEIRLVSGL
                     ELDRMDETQLAQAAASGTIFGRITPQQKERLVDALRRQGRYVAMIGDGVNDVLSLKKA
                     NLGIAMQSGSQATRSVADIVLMNDSFTALAPAVAEGQRIVNGMHDIFKLYMTRVGTVA
                     LTILSSLVIGAFPLGLRNGSLVTLLTVGIPTVLLAFWARPGQRRRGGLRRELTHFVTP
                     VAILSSLIGLLVFYGTLLLDTQWRGLSEPGLSPDQVNVATGASAALAQTALASFLTVC
                     GLFLIVFVEPPAEWWTGGDVLSGDWRPTALAVGLFLAFVAIVSTPALRAFFALEPLEP
                     VYMALIAAAVMVWMFAVRLSWRERLIERFLELNT"
     gene            6669..6739
                     /locus_tag="AC162G_05280"
     tRNA            6669..6739
                     /locus_tag="AC162G_05280"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6701..6703,aa:Gly,seq:tcc)
BASE COUNT         1177 a         2174 c         2161 g         1277 t
ORIGIN      
        1 aaatggctgg agtggtcgct caacccgaag ctgcagggcg acctggcggc gtggtttggc
       61 tcggtgcccg tcgtgctggc ggcatgcacg ggcaacgcgc tgctcaccga cgcgggatgt
      121 gagacgaacg gcctgggcaa ctttgacacg atccgcttct ggaagacacc caccgaggac
      181 tgcggcgacg gcccgggtgg catgcagggc tgcgtcccgt attatcgctg ggtcaccgac
      241 tacctcgctg tgatcggcgg acggtaacat cggcgcgcac aggctttggg agctggccgg
      301 catgcccaag gctgtgcctt ttatcgacgg ggacttcgat cgctcgtatg aacccttgcg
      361 aaggttacga accttcgcaa gggtctgcaa actcaggtga agaacacgac gtgaccaacg
      421 attccatgcc cgcagtccgg ctgtctggcg tcagccgcca cttcggcgaa gtgaaggcgc
      481 tggacaacat ccacctggac atcgtagacg gcgagttttt ctcgctgctc gggccgtccg
      541 gctcgggcaa gaccacgtgc ttgcggttga tcgcggggtt cgatcagccc acggccgggc
      601 gcatcgagct gcacggcgaa gacgtcaccg acctgccgcc gtacgagcgc agcgtcaaca
      661 cggtcttcca ggattacgcg ctgttcccgc acatgaacgt tgctgacaac gttggctacg
      721 gcctgatgat caacaaagcg cccaggctgg agcgcatgaa gcgggtgagc gaaatgctgg
      781 atctggttca gttgcccggc atggaacagc gcaagccgtc tcagctttcc ggtgggcagc
      841 gccagcgcgt cgcgctggcg cgcgcgctga tcatgcaccc gcgcgtgctg ctgctcgacg
      901 agccgctcgg cgcgctcgat ttgaaattgc gccagaagat gcagatcgag ctcaaggcga
      961 tccagcagcg tgtcggcatc accttcctct atgtaacgca cgatcaggaa gaagcgctca
     1021 ccatgagcga ccggctggcc gtgttcaacc acggccacat cgagcaaatc ggcacgccgg
     1081 ccgaggtata cgagcatccg gcgacgccgt tcgtggcggg gttcgttggc gtgtcgaacc
     1141 tgatcggcgg cgccacggcg caggcgatct tcggaacgcc cgacacgttc tcgattcgcc
     1201 cggagaagat caacatccag gcgccgggcg tcagcgtgcc ggacgggtac tgctcggtca
     1261 gcggacgcat ccgcgatgtc gtgtatctcg gcatgtacac gcgctacctg gtggagatcg
     1321 acggcggcgc cgacctgacc gtcgtccgcc agaacctcga cgagacctcg atggacgtac
     1381 tggcgaatcg agggcgcccg gtcctattga cgtggcagcg cgaacacaac cgcccgatcg
     1441 cggcgctaag ctgatcgcat gacaaccgcg tcgatgaccc ccgcagtgag ccatccctcg
     1501 gcagaacgcc gcctgtcgac gttcctgtat cgtcgcccgg tattgtccct gctgctgctg
     1561 ctgatcccgc cgctgttgtg gctgggcatt ctgtacatcg gctcgctcct ggcgctgctc
     1621 atccagagct tctatcgcct cgacggcttc accgggcggg tcgtgcgcga gttcgggttg
     1681 agcacgtacg gcgatctgct cacgcaggca cacttcgaca tcatggcccg cacggccttg
     1741 atggccgccg ccgtgacgat cgcggcggcg tttgtcgcgt ttccgatcgc ctactacatg
     1801 gcgcgctacg cggggcggcg cacgagggcc ttcctgtatc tggccgttct gcttccgctg
     1861 tggtcgagct acctggttcg ggtgtacgcc tggaagttca ttctcgccaa agagggtgtc
     1921 ttccattggt ttatctcgca gatcgggctt cagggactgc tcaatgccgt cctggacttt
     1981 cccctgatcg gcggagagtc gctgtcggtc tcgccgctcg gcctgttcct cgtgtttctg
     2041 tacgtctggc tgccgtacat gattctgccg gtcgccgcag cgctggagcg cgtgccacgc
     2101 tcgctcctcg aagcatcggg cgatctcgga gcgcgccctg aagccacatt ccagcacgtg
     2161 acgctgccgc tggccttccc cggcgtcgtg gccggatcga tcttcacctt ctcgctgacg
     2221 ctgggcgatt tcatcatccc gacgattatc ggagactcca gcccgttcat tgggctggcc
     2281 gtgtatcagt atcagggcac cgccgggaac gttccgctcg ccgccgcgtt caccatcgtc
     2341 ccgatggtga tcatggccgc gtacctgatc atcgccaaga gattgggggc gttcgatgcg
     2401 ctctaaccgc ggacgcattc tggccaaacc ctccctgggc ctgcggctgg cgacgatcgc
     2461 cgttctgttg tttctgcatt ttccgctctt aatcatcatt ctgtacgcgt tcaacacgca
     2521 agagtcggcg ttcacctttc cgccgccggg cctgacgacg gaatggttcc gggtggccct
     2581 cacggagcgt cccgacttgt ggagcgcgct gtggctgtcg gtcggggtgg cgctggtcgc
     2641 cacgctcgtc gccatcgtgc tgggcacgct catcgcagtg gccatctcgc gcagcaagtt
     2701 cttcgggcgc gaaacgatct cgttcatcgt cgtgctgccg atcgcgctgc cgggcatcgt
     2761 caccggcatc gcgctgcgct cggcgatcgg cctgatggag atacccttca ccttctggac
     2821 gatcgtgatc ggccacgcga cgttctgcgt cgtcgtggtc tacaacaacg tggtggcgcg
     2881 gctgcggcgc accgggcgct cgcagatgga ggcgtcgatg gacctgggcg cgtcgagctt
     2941 ccagacattc cgctatatcg tgctgcccaa catcgccacg gcgctgctgg cgggcgcgat
     3001 gctcgccttt gccctctcgt tcgacgagat catcgtcacg accttcaccg ccgggcagca
     3061 gacgacgctg ccgatctgga tcttcagcca gttgacgcgg ccgcgcagcc agccggtgac
     3121 gaacgtcgcg gcggtcatgg tcgtcgcgat cacgttcatc cccatcctgc tggcacaccg
     3181 cctgacgcag gacaccgaag cgtcactgta agttttcggt tttgcgttca cagggcgttc
     3241 gccgggggct tgaccgctgc catgcggcgg ggtacaatgc gcctgcattc gccggttgca
     3301 cagcctggcc aggaaggctc acacgcatgg cggttcaact acagcgcaag ctcttcacga
     3361 tagatgaata ccagcagatg atccaggccg gcgtgtttca cgaggacgat cggctggagc
     3421 tgatcgaagg ggagatcgcc gagatgtcac ccatcagtcc gaagcacgcc ggttgcgtga
     3481 accggctggc cgatctgttc tatgatcggt tgcgcaggcg ggcgatcatc gcagtgcaga
     3541 atcctgttcg cgtgaaccag cgctctgagc cgcagccgga tgtggcattg cttcgtccac
     3601 gtccgacgta ctatggaaat ttgcatcctg cacctgagga tgtgctgctt attgtcgaag
     3661 tgtctgacac atccgccgac tatgaccgcg acgtgaagat gcctatgtat gcaaaggccg
     3721 gcatcccaga agcatggctc gtgtcacttg ccgacggatg gatggaagtg tatcgagagc
     3781 catcgccagt cggttacaag tcgattcggc gtgtactgcc aggcgaaacg gtgtctccgc
     3841 tcgcgtttcc cgatgtttcg ctggatgtga gcgagatcac agaataattt ggatcagatc
     3901 ctgtttcggg gtgtagcgca gtccggtagc gcgcacggtt tgggaccgtg aagtcgccgg
     3961 ttcgagtccg gccaccccga cacatgagct gcacagacag cttaccgttc ctggtgagct
     4021 gttttgctat attcaagtgt tcaactccag aaaccgctca atgagccgct cgcgccagga
     4081 taggcgcacc gcgaacatcc acaccatcac cgccgccgca atgagggcca tatacaccgg
     4141 ctcgaggggc tccagcgcga agaaggcgcg cagcgctggc gtggacacga tcgcgacgaa
     4201 ggccaggaac aggccaacgg ccagcgcggt cggtcgccag tcgccgctca gcacgtcgcc
     4261 gccggtccac cattcggcgg gcggctcgac gaagacgatg aggaagagac cacatacggt
     4321 caggaagctg gcgagtgcgg tctgcgccag tgcggcgctg gcaccggtgg ccacattcac
     4381 ctgatcagga gacaagcccg gctcgctcag gccgcgccac tgcgtgtcca ggagcagcgt
     4441 tccatagaac accagcagcc cgatcaggct cgacaggatg gccacgggcg tgacgaagtg
     4501 cgtcagctca cgccgcaagc cgcctcgccg ccgctggccg ggccgcgccc agaacgccag
     4561 caggactgtc ggaatcccga ccgtcagcag cgtcacgagc gagccgttgc gcagccccag
     4621 cgggaaagca ccaatcacga gcgacgacaa gatcgtcagc gcgacggtgc cgacgcgcgt
     4681 catgtatagc ttgaagatgt cgtgcatgcc gttgacgatg cgctggccct ctgcgacggc
     4741 gggcgcgagc gccgtgaacg agtcgttcat caagacgatg tcggcgacgc tgcgcgtcgc
     4801 ctggctgcca ctctgcatgg cgatgcccag attcgccttt ttcagcgata gcacgtcgtt
     4861 gaccccgtcg ccgatcatcg ccacgtagcg cccctgcctg cgcagcgcgt cgacgagcct
     4921 ctctttctgc tgtggcgtga tgcgcccgaa gatcgttccc gacgcggctg cctgagcaag
     4981 ttgcgtctca tccatccgat ccagctccag gccggagacg agtcgaatct cagggcttaa
     5041 cccggcctgc ctggcgagcg cggcgacggt ctccgggtcg tcgccggaga taatcttcgg
     5101 ctcgacgcca atagcggaaa aagcggcgag cgcctcgcgc gcttccgggc gcagctcgtc
     5161 gctgagggag accagtccga ggggcgtcat gccgttcggc aggcacgatg cgtcaccccc
     5221 gtccttcaag cgaatcgggt cagggtgatg agcgacaagg agcacgcgca gcccctggct
     5281 cgtccacgcg ccggcatggg cagaaatctg ctgccacacg gccgacggcg cctcgccgtc
     5341 ggcgcccagg tagggctgca gcatctccgg cgcgcccata gcgaaaacgc cgcgccgatc
     5401 gtcttccagc gcgacagcgc tccatttgcg cgcggaggag aaaggcactt cggcgagcgt
     5461 gcgccgggga cgctccggga acgccgccgc gatggcctcg ccggttttgt tgcgcgccgc
     5521 cgcgctcgcg gccatcgcgc caagaacgct ctgcaattcc tgttcgccga ccccgatggc
     5581 gtgcaactgg ctgacctgca accggttggc ggtaagcgtt ccggtcttgt ccaggcacag
     5641 cacgtccacg ctgctcagtg actcgacggc gtttgactgc tggacgagcg cgccgaagcg
     5701 gatgatgcgc acggcagcca gcgcgtaggt gatggcgatc gacacgaaca gcccgttcgg
     5761 caccagactc gcgatcatcg tcgcctgggt cacgctttcg gcggccggca ccagcttcac
     5821 gacggcattg accaccagca gcgcctcgat gtaaatgatg atgagcagca tgacgcgaat
     5881 cacgaggtga atctgccgct gcagcggcgt gagcacgcgg cgaaaggcgc gcgccccgga
     5941 cgtgatctgg ttggccaggc tcgcctcacc gactttctcg gcgacgtata cggcgctgcc
     6001 actcatgcag aagctgccgg aataaaccgg atcgcccggc tgcttgaaga tcagattcga
     6061 ttcgccggtg agctgcgact cgtcgacgga catgcgcccg tcgatcaccg cgccgtcgag
     6121 cacgatctga tcgcctgcgc tgactttcaa cacgtcgcct acaaccagct cttccggcgc
     6181 gatcgggcgc tcttgtccgt cgcggatgat cctcgcttgg ggacgggtga gcagcgctat
     6241 ccgatcgagc gtgcgcttgg cgcgaatctc ttgcacgacg ctgacggcga tgttcgtcgc
     6301 gatgacgccg acggagagga tcgcgtcgag cggccggccg acgagcacga gcgcgagcgc
     6361 caggaggaac aggacattgt tgatgaaggt gaagacgttt tcgcggacga tctggacgta
     6421 ggtgcgcccg gtggcgggcg gcacgcggtt gccctgccct ttcgcccggc gcgcctgcgc
     6481 ttcggtctcg gatagtcccc gaaggggcaa tgcagtcaca tcgagtgtca tcacgtccac
     6541 ctccgatccg tcggcagggc gcggctgttt cagccatgct gctgtccgat cttgaactgc
     6601 cttgaagaat tctgtggtat tataatgccg gttgggcgat attggcgtag ccgcatgatt
     6661 cgatctgtgc gggagtagct caggggtaga gtatctgcct tccaagcaga agacgcgggt
     6721 tcgattcccg tctcccgctc ctaaaatata cgacagggag gaacagtcac aaccagatgt
     6781 ggcggttcc
//
LOCUS       DBNHXB010000137        15078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_27397, whole genome shotgun sequence.
ACCESSION   DBNHXB010000137 DBNHXB010000000
VERSION     DBNHXB010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 15078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15078
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_27397"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="AC162G_05285"
     CDS             <1..346
                     /locus_tag="AC162G_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR5075258.1"
                     /translation="SAIALAIGIGIQNFPEGTAVAMPLRREGVSRWRSFLYGQASALV
                     EPIAAVAGAAFVLLAQPLLPYTLSFAAGAMIFVVVEELVPEAQGGGNTDLATMGTMIG
                     FAAMMMLDVAFG"
     gene            620..1519
                     /gene="folD"
                     /locus_tag="AC162G_05290"
     CDS             620..1519
                     /gene="folD"
                     /locus_tag="AC162G_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR5075259.1"
                     /translation="MTAQIIDGKAIADKVRRQVAEEAAQFRDKTGVQPGLATVLVGDN
                     PASGTYVRSKQKACEELGMRSVGHHLPADATQEQVEGLVRSLGADPAVHGILVQLPLP
                     SQLNEEAVLNAVPIDKDVDGFHPVNIGRLAMKGRKPVFVPCTPAGCIVLLDEAGVPIK
                     GANAVVVGRSNIVGMPAALLLVHRDATVTIVHSRTRDLPGVVRQADILIVAIGKAHFV
                     TGDMIKPGAAVIDVGINSIPDPTKKSGQRLVGDVDFESAKEVAGYITPVPGGVGPMTI
                     AMLMRNTLEAAKHAVEMGVSVGS"
     gene            1550..1903
                     /locus_tag="AC162G_05295"
     CDS             1550..1903
                     /locus_tag="AC162G_05295"
                     /inference="COORDINATES: protein motif:HMM:NF052504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sll1863 family stress response protein"
                     /protein_id="HIR5075260.1"
                     /translation="MSTANPRKPIKEEPEAPPDLEELLAEAKSGKWEDQLKEWGIDLE
                     KLKARAEKAGGDARVEFDRQAQTLRPMMSQAQAELVELRKLGGEASVEMLKGVDSAFT
                     ELKKAFASAAAKFKP"
     gene            complement(1893..2702)
                     /locus_tag="AC162G_05300"
     CDS             complement(1893..2702)
                     /locus_tag="AC162G_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HIR5075261.1"
                     /translation="MPPYTWPDCPEDVRRQVGSLTNGLCRILADNLIGIYLHGSLAMG
                     GFNPQRSDVDLLVVARRGMRVQTKREVAGLLLRCSNDPRPIEISFLSQAQLRPWRHPA
                     PFDFHFGEDWREELEAQMADGRWLAWNDEIRVDKDLAAHITVTLSRGICLYGAPFAEV
                     FPPVPHEDYLDSILSDVDWARERIVENPVYFVLNACRVLAYLSEGLILSKDEGGAWAL
                     AGLPSEFHPTIAVALDIYRGEAEPGALRAECVAPFAAHVTNRAGARPAPTA"
     gene            2836..4038
                     /locus_tag="AC162G_05305"
     CDS             2836..4038
                     /locus_tag="AC162G_05305"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR5075262.1"
                     /translation="MTNVKKHKGVIGILTGGGDVPGLNPAIRAVTIRALREGHQVIGI
                     RRGWAGAMSIIRDHDADNSDSYQVLTEEIVNKAGRTGGTFLHTSRTRPSHVPKASVPE
                     HLQEAYNDKANDLTPEVLQNLDFLGIDYLIPIGGDDTLSYAVDLYKKGVKIVAIPKTM
                     DNDVPGTDYCIGFSTCVTRTIQMTNSLRTIAGSHERFLVLEVFGRYAGFTAMLPTMAG
                     AAHRCVIPEYKFNIELLTELMCADRRMNPSQYSVVLVSEGAMFEGVEMIFESAEKDAY
                     GHMKLGGIGDVVAAKLKDLSPAYNHGKPVNVVNQKLGYLVRGGDPDAIDSIVPMAYGN
                     LALDLIEKGVHGRLVVLKNGRYDNMPLDVVTSSKKVVNVEKYYNKERLRPFYKSFEMK
                     PLFIMTGD"
     gene            complement(4153..5436)
                     /locus_tag="AC162G_05310"
     CDS             complement(4153..5436)
                     /locus_tag="AC162G_05310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5075263.1"
                     /translation="MSILRRYRRRLIASPPARFLDQFNRDARLFLLATVIGGITFTGF
                     QLFFNIYLRSRGFDLDFIGVLNAIPSGAALLAGVPMGMLSDRIGPRRAMLAGLGVAAL
                     AASALVTARTPFVMIVMSGVMGMGNSLYFLSMAPFMMRASSDKERTMLFSLNFGLMTI
                     SGAVGNLLAGQLPAWFARWLGVPPGSPATYQAVLLASALVGGLALIPIWLIREARWPG
                     PRRTAPVGLGEVRHLLRRSVLRMALPNFVIGFGAAILIPYLNLFFKETFAISDGQLGL
                     VFSLSAAVTGVASLVGPRIAERLGSKIKAVVATQGASVLFLLMMGFVPAYWIAAVAFL
                     IRGALMNMSNPLYSTFTMEQTPERERGTVNSMLQLTWEVGWTVGPYLSGVVQARYGFA
                     PLFLGTATLYSLAIGLTWLFFHDTEARAKAAAATA"
     gene            complement(5489..6841)
                     /locus_tag="AC162G_05315"
     CDS             complement(5489..6841)
                     /locus_tag="AC162G_05315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5075264.1"
                     /translation="MIQDTTEQHTILHDLGDGLILRRSTPADADALAEFNARIHSDAG
                     PDQPDEWVGEWTRDLLTSDHPTFGAGDFTLVEDMRAGKIVSSLNLISQTWTYDGIPFG
                     VGRPELVGTLPEYRNRGLVRAQFEVIHAWSAGRGERVQAITGIPWYYRQFGYEMAMDL
                     GGGRLGFLPHIPKLKEGETEPYPIRPATESDLPFIMQVYAQAVTRYRVACLRDEALWR
                     YEMSGRSEKNANGRRFCIIESADGEPAGFLAHAGRLWRMSFPINLYELKPGVSWLAVT
                     PGVIRYAQVTGEAYAARDGTEPLGGFGFWLGGDHPAYHAIEDRLPRVRHPYAWYLRVP
                     DLPGFLAHIAPSLEKRLAGSIAAGYTGELKISFYRTGLRLVVDKGRLTGIEPWTPRRG
                     DDGVVAFPDLTFLQLVFGYRTLDELRYAFADCWVDTDEARAVLEAMFPKQSSDVWPVA
                     "
     gene            complement(6922..7797)
                     /locus_tag="AC162G_05320"
     CDS             complement(6922..7797)
                     /locus_tag="AC162G_05320"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR5075265.1"
                     /translation="MNQERAYLQRIHEAYPHLVVKTARLHTRDGQFSDILFVDEELIF
                     RFPRSPHVAKEMAFEMAILSRLQRHMSLPIPNPVYHRADLQTGDLIFIGYRMIPGEPL
                     SRETVKAIGDETALDGMATQLATFMRELHGVPVAELGLDVPVRDGHECWADIYARFRE
                     HLFPHMRPDAREQVARGFETFLSDPVNFAYRPVLRHGDFGGANILFDPKMQSIAGVLD
                     FSFIALGDPAFDLASISCFGESFAQRIVRADRRLEPMRDRARFYRSTFALLQALYALR
                     DNDLESFKGGMAQYI"
     gene            complement(7848..8822)
                     /locus_tag="AC162G_05325"
     CDS             complement(7848..8822)
                     /locus_tag="AC162G_05325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5075266.1"
                     /translation="MLGDNWIIHPPVMADLDAVVDLINACARADIGTDNITPARLRTL
                     WTDERFQLATDAWIVATRDGRLAGYADFGDEEPPETYQMSGYVHPDYGGRGIGTHLLG
                     LIEQRARQAMIDAPPDLRIALQNGIYATNAGAHELLEHEGYALVRRWYKMLIQIDAPP
                     PAPKVPEGITIRTFRPGEEDRAVHAAYEEAMADEWEHPPLSYERWRHYKIDTEPNFDP
                     TLWFLAMDGDQIAGLALCRWERPGEPDQGHVRDIGVRPTWRRRGIGLALLRYTFGEFY
                     RRGKRRVGLGVDATSLTGAERLYCKAGMHVLLETLIYEKELRPGKREA"
     gene            complement(8886..9899)
                     /locus_tag="AC162G_05330"
     CDS             complement(8886..9899)
                     /locus_tag="AC162G_05330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5075267.1"
                     /translation="MITQLEATNILPLPLDWPEYDWRPLRREDLPALHRMLLAADEAD
                     DTESADTLQDLQTQFDDPWSDPETDSIAALTRDGEVAALARTFMNPEPEEECRAFLSD
                     QVHPEHRGRGLEEFVLSWAEARGHQRLAALPSTLPRLLRSFSQDTLRDRIERLERRDY
                     RPVRYFYRMRRDLRQPIPDRPLPDGLTLRTFSPELSRPVLDALNEAFRDHWSFEPVSD
                     EDWQMFFLQRSSFRPDLTFVAMDGGEVAGLSFNTISPDENARHGIEEGWVAELAVRRP
                     WRRRGVASALLCETMRAFKAEGLDYATLGVDTENLSGALGLYEGLGFVPVKRFVAFEK
                     RMV"
     gene            complement(10037..11077)
                     /locus_tag="AC162G_05335"
     CDS             complement(10037..11077)
                     /locus_tag="AC162G_05335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5075268.1"
                     /translation="MTTQILESPAVTLEGGYLTRPAALGDVEAAVAVFNACSRQLLGV
                     DEFNADNYRVEWEIPGLNLETDVRVVVAPNGKVVACMEVWDLFDSHVRVNFFGRVHPD
                     HQGRGIGTALVRWAEARARQAVDKAPEGTRVFMLTGVLDANRAAQELFRSEGFELVRH
                     HWRMVIEMDAHAPPPAPGLQDGIVVRPLRFGQDDRAAIQAVRDSFRDHWGYVEQPFEE
                     ELERWQHFMKNDKDFDPSIWFLAWDGGEVAGVSLCWPKSNDDPDMGWVGTLGVRRPWR
                     RKGLGLALLHHSFGEFYRRGCRRVGLGVDAHSLTGATRLYEKAGMRVARVYNTFEKEL
                     RAGVDLSTQSVH"
     gene            complement(11150..11974)
                     /locus_tag="AC162G_05340"
     CDS             complement(11150..11974)
                     /locus_tag="AC162G_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5075269.1"
                     /translation="MSVITQELKASYAFIERNWNIVKRYWAWELVWLVYTVANSLAVS
                     YIGLGMEQIGGPGVDGKYLVLYLAIGTIVWRYLAVIFYWVTDVVGLERWEGTIEYTLM
                     APITRLMHMLGQTAFAIVYSLAHTGVVLLATMLLFEIRLDHANMWGFLLLLIAGSLSF
                     IGISIVGSTLPLLFPERGSQMTHLIIAMLLLVSGVYYPVNVLPAWLQVFSKLSPATYV
                     LDGSREALLNGAPTESLWGYIAPLLVMGVIAIPIGLRIFQWAERYAKRTGKLHRNG"
     gene            complement(12053..12613)
                     /locus_tag="AC162G_05345"
     CDS             complement(12053..12613)
                     /locus_tag="AC162G_05345"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5075270.1"
                     /translation="MCAQHLRGDHVRLVALQSDSDIELLARWSAMSALWRLLDTATGR
                     PMQPGSNEHAGFLIYPLVGDRPIGHAGLFGIDRRRGEAWLGIGLADHDRWDTPAGADA
                     LRVIVRYGFDELNLRRISLAVFEYNARALRAYESAGFAIEGRMLQEASRRGRSRAGVY
                     MSLPREEWERQTEHASPAPQSVISEQ"
     gene            complement(12683..13237)
                     /locus_tag="AC162G_05350"
     CDS             complement(12683..13237)
                     /locus_tag="AC162G_05350"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018753591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5075271.1"
                     /translation="MDMQIFKGELVRLTASISDKDAETLASWSRDSEFQRLLDDDPAQ
                     PRSVKKVKEEIEEEPKHDRFPFLIRTLADDRLIGFVGLGGVRWTHRDAWVGIGLGDRE
                     YWGKGYGTDAMRVVLRYAFAELNLHRVTLGVFEYNARAIRSYEKAGFVVEGRSKQEVL
                     RDGRRWDGIYMGILRDEWEKKRNE"
     gene            complement(13358..13915)
                     /locus_tag="AC162G_05355"
     CDS             complement(13358..13915)
                     /locus_tag="AC162G_05355"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5075272.1"
                     /translation="MHSQLFEGECIRLAPIDPEKDAETISKWTHDPEYLRLTDAEPAR
                     PLSPGQVKKKLEEMDKEAEKRTQFNFAIRLRDGDRLIGLASIRWIHWTHSAGWIQLSI
                     GRPEDRGKGYGSEALKMSLRYAFDELNLHRLGAVTLEDNPSALRFLERNGFVVEVRRR
                     QAIYRDSRRWDALMLGLLREEWISD"
     gene            complement(14017..14859)
                     /locus_tag="AC162G_05360"
     CDS             complement(14017..14859)
                     /locus_tag="AC162G_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5075273.1"
                     /translation="MNDTIAVQIDGALKTFGGKGSRLFAAKNGKNGNGRLPEGAKVAV
                     NRVSLDVKRGEIFGILGPNGSGKSTLIRLIATLLVPDDGRIAVFGHDVVAEPRRVQTM
                     INRVSVEASFFKKLSPMENLLYGARLYGVGASEARTRVRDILARLGLESRSIDRPMEE
                     MSRGMQQKVAIARAFLSSPIVLLLDEPTTGLDPRSKREVQAFVRELRDVHDTTILLTT
                     HDMYEADALCDRIAIIDGGKIVALGTPQELKRAIPRNGHEPTLEEVFLELTGKQLVAE
                     EEAG"
BASE COUNT         2853 a         4843 c         4660 g         2722 t
ORIGIN      
        1 gtcggccatc gcgctggcga tcggcatcgg catccagaac tttcccgaag gcacggcggt
       61 ggcgatgccg ctgcgccgcg aaggtgtatc gcgctggagg agttttctgt acgggcaggc
      121 gtcggcgttg gtggagccga tcgcggcggt ggcgggcgcg gcgttcgtcc tgctggcgca
      181 gcccttgttg ccttacacgc tgagcttcgc ggccggcgcg atgatcttcg tcgtggtgga
      241 agagcttgtg cctgaggcgc agggcggcgg taataccgac ctggcgacga tggggacgat
      301 gatcggtttc gcggcgatga tgatgctgga cgtggcgttc gggtagcggc gcccaccgta
      361 gagcgtgttt ccatacgcgc cgcaaattgg cggctatgga aagccgcccc tacgtccggc
      421 ctgacatctt ttgcgtgccc acaatcgccg ttcgacgccg cgtttgtgac cttcgttcag
      481 aaaagccaat aaccacggcg tttcttttca tcatgcccgc aggatgacaa agcacactcc
      541 cccggcgcga gcggatatgc tatcctatgc agcgcgcttc tacactgcgc gcagtcgaac
      601 tcacctcagg aggctttccg tgacagcgca aattatcgac ggcaaagcga tcgcggataa
      661 agttcgcagg caagtggccg aggaggctgc gcagttccgg gacaagacgg gcgtccagcc
      721 cgggcttgcg acggtgctcg tcggcgacaa tccggccagc gggacgtatg tgcgatccaa
      781 gcagaaggcg tgcgaggaat tgggcatgcg ctcggtcggg catcacctgc ccgccgacgc
      841 gacgcaggag caggtggagg ggctggtgcg ctcgctgggc gccgacccgg cggtacacgg
      901 catcctggtg caactgccgc tgcctagcca gctcaacgag gaggcggtgc tgaacgcggt
      961 gcccatcgac aaagacgtgg acggctttca tccggtcaac atcgggcggc tggcgatgaa
     1021 agggcgcaag ccggtcttcg tgccgtgcac gccggcaggc tgcatcgtgc tgctggacga
     1081 agcgggcgtg ccgatcaagg gcgcgaacgc ggtcgtcgtc gggcgcagca acatcgtggg
     1141 catgcccgcc gcgctgctgc tcgtgcatcg cgacgcgacg gtgacgatcg tccactcgcg
     1201 cacgcgcgat ctgcccggcg tcgtgcgaca ggccgacatt ctgatcgtcg ccatcggcaa
     1261 ggcccatttc gtcaccggcg acatgatcaa gcccggcgcg gcggtgatcg acgtgggcat
     1321 caacagcatc cccgatccga cgaagaagag cggccagcgg ctggtcggcg acgtggactt
     1381 cgaatcggcg aaagaggtgg cagggtacat cacgccggtt ccgggcggcg tcggcccgat
     1441 gacgatcgcg atgctgatgc ggaatacgct cgaagcggcg aagcacgcgg tcgagatggg
     1501 agtgagtgtg gggagttagt gcgaggagtc agagttggag gtggtagcga tgagcactgc
     1561 gaatccacga aaaccgatca aagaagagcc ggaagcaccg cccgatctgg aggagctgct
     1621 ggccgaggcc aagagcggca agtgggaaga tcagctcaag gagtggggca tcgatctgga
     1681 gaagctcaag gccagagccg agaaggcggg aggcgacgcc agagtcgaat tcgacaggca
     1741 ggcccagacg ctccgcccca tgatgagcca ggcgcaggcg gagctggtcg agctgcggaa
     1801 gctcggtggc gaagcctcgg tcgagatgct gaaaggcgtt gacagcgcct tcaccgaact
     1861 gaagaaggcg ttcgcaagcg ccgccgcgaa attcaagccg taggggcagg ccttgcgcct
     1921 gcccggttcg tcacatgcgc ggcgaatggc gccacgcatt ccgcgcgcaa ggcacctggt
     1981 tcggcctcac cccgatagat gtccagcgcc accgcgatgg tcggatgaaa ttccgatggg
     2041 aggccggcta gcgcccacgc gccgccctcg tccttcgaga ggatcagtcc ctcgctcaag
     2101 tacgcgagca cgcggcacgc attcaggaca aaatacacag gattctccac gatacgctcc
     2161 ctcgcccaat caacatccga caggatcgag tcgaggtagt cctcgtgcgg aacgggaggg
     2221 aagacctccg cgaacggcgc gccgtacagg cagatgcccc ggctcagtgt caccgtgatg
     2281 tgcgcggcga ggtccttgtc cacgcgtatc tcgtcgttcc atgccagcca tctgccgtcg
     2341 gccatctgcg cctcaagctc ctctcgccaa tcttcgccga agtgaaaatc gaacggcgcc
     2401 ggatgccgcc aggggcgcag ttgcgcttga ctcaggaagc tgatctcgat cgggcgcgga
     2461 tcgttagagc agcgcagcag caatccggcg acctcgcgct ttgtctgcac acgcatgccc
     2521 cggcgggcca cgacgagcag gtcgacgtcg ctgcgctggg ggttgaagcc gcccatcgcc
     2581 agcgaaccgt gcagatagat accgatgaga ttatcggcca gaatcctgca cagtccgttg
     2641 gtcagcgagc cgacctgccg gcgcacgtct tcagggcagt caggccacgt gtacgggggc
     2701 atggcacaag aagtcaatca cggaggcgcg gagactgcga agcatcttat tttgccgcgc
     2761 ccatccgtcc ataactttct ggctttctga gtgcgtggta aacttaatga ttggattctg
     2821 tagggagaga taagcatgac aaacgtcaag aagcacaaag gtgttatagg aattctgacc
     2881 ggcggcggcg acgtgcccgg gctgaatccg gccattcggg ccgtgacgat ccgggcgctg
     2941 cgcgagggcc accaggtcat cggcattcgt cgcggctggg ccggcgcgat gagcatcatt
     3001 cgcgaccacg atgccgacaa cagcgacagt tatcaagtcc tgaccgaaga aatcgtcaac
     3061 aaggcggggc ggacgggcgg cactttcctg cacacgtcgc gcacgcgccc cagccatgtg
     3121 ccgaaggcga gcgtccccga gcacctgcag gaagcttaca acgacaaggc gaacgacctg
     3181 acgcccgagg tgcttcagaa tctcgacttc ctgggcatcg actacctgat tccgatcggc
     3241 ggcgacgaca cgctgagcta cgcggtcgat ctgtacaaga agggcgtgaa gatcgtagcc
     3301 atccccaaga cgatggacaa cgacgtgccc ggcacggact actgcatcgg cttcagcacg
     3361 tgtgtgacgc gcacgattca gatgacgaac agcctgcgca cgatcgccgg ctcgcacgag
     3421 aggttcctgg tgttggaggt cttcgggcgt tacgccggtt tcacggcgat gctgccgacg
     3481 atggctggcg cggcgcatcg ctgcgtgatt ccggagtaca agttcaacat cgagcttttg
     3541 accgagctga tgtgcgccga tcgccggatg aacccgagcc agtactcggt ggtgctcgtg
     3601 tcggagggcg cgatgttcga aggcgtcgag atgatcttcg agagcgccga gaaagacgct
     3661 tacgggcaca tgaaactggg cggcatcggc gacgtggtgg ctgccaagct caaggacctc
     3721 tcgccggcgt acaaccacgg caagccggtc aacgtggtca accaaaagct gggctatctg
     3781 gtgcgcggcg gcgacccgga cgcgatcgat tccatcgtgc cgatggccta cggaaacctg
     3841 gcgctggatc tgatcgagaa aggcgttcac ggccgcctgg tcgtcctcaa gaacgggcgg
     3901 tacgacaata tgccgctcga cgtggtgacc agctcgaaga aggtcgtcaa cgtggagaag
     3961 tactacaaca aagaacgcct gcgcccgttt tacaagagct tcgagatgaa gccgttgttc
     4021 atcatgacgg gcgactaaca gacaacgtca aactccagaa tccaaacttc aaatcgggcc
     4081 ggcccgcgcc gtggacagcg cggagagcta tcagctatct gccatccgcc gtcagccatt
     4141 cgccatctgc cattacgccg tcgctgccgc cgctttcgcc cgcgcttcgg tgtcgtggaa
     4201 gaatagccac gtcaggccga tggccagact atacaacgtc gccgtgccga ggaaaagcgg
     4261 tgcgaagccg taacgcgcct gcaccacgcc cgaaagatac ggccctaccg tccagccgac
     4321 ctcccacgtc aactgaagca tactgttcac cgttcctcgc tcgcgctccg gcgtctgctc
     4381 catcgtgaac gtcgagtaga gtgggttgct catgttcatc agcgcgcccc ggatcaggaa
     4441 ggcgactgcc gcgatccagt acgcgggcac gaagcccatc ataagcaaga acaagacgga
     4501 tgcgccctgc gtggcgacga cggccttgat cttgctgccg agtcgctcgg cgatgcgcgg
     4561 gccgacgagt gaggccacgc cggtgaccgc ggccgacagg ctgaacacca ggccgagctg
     4621 gccgtcgctg atggcaaacg tttccttgaa gaaaaggttg aggtacggga tcaagatcgc
     4681 tgcgccgaag ccgatgacga aattcggcag cgccatgcgc agcacgcttc gcctcagcag
     4741 gtgccgaacc tcgcccaggc cgaccggcgc ggtgcgccgt gggccaggcc agcgcgcctc
     4801 gcgaatcagc cagatcggaa tcagcgccag tccgcccact agagcgctgg ctaacagaac
     4861 ggcctggtat gtcgccggcg agcctggcgg cacgcccagc caccgcgcga accacgccgg
     4921 caactggccg gccagcaggt tgccgaccgc gccggagatc gtcatcagcc caaagttgag
     4981 actgaacagc atggtgcgct ctttgtcgct cgacgcgcgc atcatgaacg gcgccatgct
     5041 caggaagtac agcgagttgc ccatgcccat gacgccggac atgacgatca tcacgaacgg
     5101 cgtgcgcgcc gtgacgagcg ccgaggcagc cagcgcggcc acgcccaatc cggccagcat
     5161 cgcgcgccgt ggcccgatcc gatccgacaa catgcccatc ggcacgccgg ccaggagcgc
     5221 cgcgccggat gggatggcgt tcagcacgcc gatgaagtcg aggtcgaagc cccggctgcg
     5281 caggtagatg ttgaagaaaa gctggaaccc tgtgaaggtg atcccgccga tgaccgtcgc
     5341 cagcaagaac agccgtgcgt cgcggttgaa ctgatcgaga aaacgggccg gcgggctagc
     5401 aataagtcgc ctgcggtatc tgcgcagaat gctcatggcc tctccacgtt cgagtatttc
     5461 tccccgtcgg ctcgcgccag caacccgatc acgccaccgg ccacacatcc gaactctgct
     5521 tcgggaacat cgcctctagc acggcgcgcg cctcgtccgt atcgacccag cagtcggcaa
     5581 aagcatacct caattcgtcg agtgtccggt aaccgaacac cagttgcagg aaggtcaggt
     5641 cggggaaggc gaccacgccg tcgtcgccgc gccggggcgt ccacggctcg atgcccgtga
     5701 gccggccctt gtccaccacc aggcgcagcc ccgttcgata gaaactgatc ttcagttcgc
     5761 cggtgtagcc ggcggcgatc gatccggcca ggcgcttctc cagcgacggc gcgatgtgcg
     5821 cgaggaagcc cggcaggtcg ggcacgcgca gataccaggc gtacggatgc cgaacgcgag
     5881 gcaggcgatc ctcgattgca tgatacgcgg ggtgatcgcc gcccagccag aaaccgaagc
     5941 cgccgagcgg ctctgtgccg tcgcgcgccg cgtaggcttc gcccgtcacc tgggcatagc
     6001 ggatcacgcc gggcgtcacg gcaagccagg acacgccggg cttcaactca tacaggttga
     6061 tggggaagct catccgccat agccgtcccg cgtgcgccag gaagccggcc ggctcgccgt
     6121 ctgcggattc gatgatacag aagcgccgcc cgttggcatt cttctcgctc ctgccggaca
     6181 tctcgtagcg ccacagcgct tcgtcgcgca ggcacgcgac gcgatatcgc gtgacggcct
     6241 gggcgtatac ctgcatgatg aacggcaaat cggattcggt cgcgggccga ataggatacg
     6301 gctcagtctc gccttctttc agcttgggga tgtgaggcaa gaagccgagc cggccgccgc
     6361 cgaggtccat cgccatctcg tagccgaact ggcggtaata ccacgggatg ccggtgatgg
     6421 cctgcaccct ctcgccgcgc ccggcgctcc acgcgtggat cacttcgaac tgcgcgcgca
     6481 ccaggccgcg attgcggtac tccggcagcg tgccgaccag ctccggccgc ccgacgccaa
     6541 agggaattcc gtcatacgtc cacgtctgcg agatcaaatt gagggaggag acgatcttgc
     6601 ccgcgcgcat gtcttcgacg agagtgaagt cgccagcgcc gaaggtcggg tgatcgctgg
     6661 tgagcagatc tcgtgtccat tcgccgaccc attcgtcggg ctgatcgggg ccggcgtcgc
     6721 tgtggatgcg cgcattgaac tcggcgagcg cgtcggcgtc cgccggcgtc gatcggcgca
     6781 agatcaggcc atcgcccaga tcgtggagaa ttgtgtgttg ttcggtggtg tcttgaatca
     6841 tcagcatatc ccatcacgtg ttccgtgttg cgtattccgt ttcatcgacc tttgatgaac
     6901 acctacacct gcagttcacg ctcaaatata ctgcgccatc ccgcccttaa atgactctag
     6961 atcgttgtcg cgcagcgcgt acagtgcctg cagcagcgcg aacgtgctcc ggtagaagcg
     7021 ggcgcgatcc cgcatcggct ccaggcgccg gtcggcgcgt acgatccgct gcgcgaatga
     7081 ttcgccgaag caggagatcg acgccaagtc gaacgccgga tcgcctaaag cgatgaaact
     7141 gaagtccagc acgcccgcaa tcgattgcat cttcggatcg aagaggatgt tggcgccgcc
     7201 gaaatcgccg tgcctcagca caggccgata ggcaaagttg accggatcgc tcagaaatgt
     7261 ctcgaagcca cgtgccacct gctcgcgggc atctgggcgc atgtgtggga agagatgctc
     7321 gcggaagcgc gcgtatatgt cggcccagca ctcgtggcca tcgcgcactg gcacgtcgag
     7381 tcccagttca gcgacgggca caccgtgcaa ctcgcgcatg aacgtcgcca gttgagtcgc
     7441 cattccgtcg agcgcggttt cgtcgccgat tgccttgacc gtctcccggc tcagtggctc
     7501 gccggggatc atccggtagc ctataaagat cagatccccc gtttgcagat cggcgcggtg
     7561 gtaaaccggg ttggggatcg gcagcgacat gtgccgctgc agccggctga gaatcgccat
     7621 ctcaaacgcc atctctttgg cgacatgagg cgaacgcgga aagcggaaga tcagctcctc
     7681 atccacgaac aggatgtcgc tgaactgccc gtcgcgcgtg tgcagacgag ctgtcttcac
     7741 caccaggtga ggataggctt cgtggatgcg ttggagatat gccctttcct gattcatgga
     7801 ttgccccctg aaccatcgcc aaagaacggg agatgtctat cgtgaaatca cgcttcacgt
     7861 tttccgggcc gcaattcctt ctcgtagatc aatgtctcca gcagcacgtg catgcctgcc
     7921 ttgcaataca ggcgctccgc tccggtcagg ctggtcgcgt cgacgccgag gccgaccctg
     7981 cgcttgccac gacgatagaa ctcgccgaac gtgtagcgga gcagcgccag tccgatgccg
     8041 cgccgccgcc aggtgggccg cacgccgatg tcgcgcacgt ggccctgatc tggctccccc
     8101 ggtcgctccc agcggcacag cgccagcccg gcgatctggt cgccgtccat agccagaaac
     8161 cacagcgtcg gatcgaagtt cggctctgtg tcgatcttgt agtgccgcca tcgctcgtac
     8221 gagagcggcg gatgctccca ctcgtccgcc atcgcctctt cgtaagctgc atgcacggcg
     8281 cgatcttcct cgccgggccg aaacgtgcgg atggtgatcc cttccggcac tttcggcgca
     8341 ggcggagggg catctatctg gatcagcatc ttgtaccacc gccgcacgag cgcgtagcct
     8401 tcgtgctcca gcaattcgtg cgcgcccgcg ttcgtggcgt agattccgtt ctgcagtgcg
     8461 atgcgtaggt cgggtggcgc gtcgatcatt gcctgcctcg cgcgctgctc gatgagacct
     8521 aacaggtgtg tgccgatgcc acgcccaccg tagtcgggat gcacatagcc tgacatctga
     8581 tatgtctcgg gcggctcttc gtcgccgaag tcggcgtagc cggcaagacg cccatcacgg
     8641 gtggccacaa tccaggcatc cgtcgcaagc tggaagcgct cgtcagtcca cagcgtgcgc
     8701 aggcgagcgg gtgtgatgtt gtccgtgccg atgtcggcgc gggcgcaggc gttgatgagg
     8761 tccaccactg cgtcgaggtc ggccatgacc ggtgggtgga tgatccaatt gtcgccgagc
     8821 atcctctttc tcccgcgtga tacgtgagat gcgaacgccc cgcatcacgt atcacgcttc
     8881 atgtgtcaaa ccatccgctt ctcgaatgcg acgaagcgct tgacgggcac gaacccgagt
     8941 ccctcgtaga ggcccagcgc gccggagagg ttctccgtgt ccacgccgag cgtcgcgtag
     9001 tcgaggccct cggccttgaa ggcgcgcatc gtttcgcaca gcagcgccga agcgacgccg
     9061 cgcctgcgcc acggccggcg cacggccagc tcggccaccc agccttcttc gattccgtgc
     9121 cgcgcgttct cgtcggggct gatcgtgttg aagctcagcc cggccacctc gccgccgtcc
     9181 atcgccacga acgtcaggtc gggacggaac gacgagcgct ggaggaagaa catctgccaa
     9241 tcctcgtcgc tgaccggctc gaagctccag tgatcgcgga aggcttcatt gagcgcgtcc
     9301 agaacgggcc ggctcaattc cgggctgaac gtgcgaagcg taagaccgtc gggcagaggc
     9361 cggtcgggta tcggctggcg cagatcgcgc cgcatgcggt agaagtatcg caccgggcgg
     9421 taatcgcgtc gctccagccg ctcgatacga tcacgaaggg tatcctggct gaagctgcgc
     9481 agcaggcgcg gcaaggtcga gggcagcgcc gccaggcgct ggtggccgcg cgcttcggcc
     9541 cagctcaaga cgaactcttc caggccgcgc cctcggtgct cggggtgaac ctgatccgag
     9601 aggaaggcgc ggcactcctc ttccggctcc gggttcatga acgtgcgcgc gagcgccgcc
     9661 acctccccgt cgcgcgtcaa cgcggcgatc gaatcggttt ccggatcgga ccacggatcg
     9721 tcgaactgcg tttgcaggtc ttggagcgta tccgccgatt ccgtgtcgtc ggcctcgtcc
     9781 gcagccaaca gcatccgatg cagcgccggc aggtcttcac ggcgcagcgg gcgccagtcg
     9841 tactccggcc aatcgagcgg gagtggcaat atgttcgttg cttccagttg cgtaatcata
     9901 tctgatcctt ttgagttgcc catggtgggt ggtcagtgat cggtgatgag tgatcgtaat
     9961 tggtaattag taattggtgg tatctaaatc actaatcatc aattactgat tactgatcac
    10021 ttcttactga tcactgtcag tgtacagact gcgtgctcaa gtccacaccc gctcgcaatt
    10081 ccttttcgaa ggtattgtac acgcgcgcga cacgcatgcc ggccttctcg tagaggcgcg
    10141 tcgcgccggt caggctgtgt gcatcgacgc ccagtccgac gcgtctgcaa ccgcgccgat
    10201 agaattctcc gaacgagtgg tggagcaggg cgaggcccag cccctttcgc cgccacggcc
    10261 ggcgcacgcc tagcgtgccg acccatccca tgtccgggtc gtcgttcgac ttgggccagc
    10321 acagcgacac gccggctact tcgccgccgt cccacgccag gaaccagatc gagggatcga
    10381 agtccttgtc gtttttcatg aagtgctgcc agcgctcgag ctcttcctcg aacggctgct
    10441 cgacgtagcc ccagtgatcg cggaacgagt cgcgcacagc ttgaatcgcc gcccgatcgt
    10501 cctgcccgaa gcgcaggggt cgaaccacga tgccgtcctg cagcccgggc gcgggcggtg
    10561 gcgcatgggc gtccatttcg atcaccatgc gccagtggtg tcgcaccagc tcgaacccct
    10621 ccgagcgaaa aagctcttgc gcagcccgat tggcgtcgag aacgccggtg agcatgaaca
    10681 cgcgcgtgcc ctcgggcgct ttgtcgacgg cctggcgcgc ccgcgcctcg gcccagcgca
    10741 ccagcgccgt gccgatgcca cgcccctggt gatcgggatg gacgcgcccg aagaagttga
    10801 cgcgcacgtg cgagtcgaac aggtcccaga cttccatgca ggcgacgact ttgccgttgg
    10861 gcgcgacgac gacgcgaacg tcggtctcca gattcaggcc cgggatttcc cactcgacac
    10921 ggtagttgtc ggcgttgaat tcatccacgc cgagcaactg gcgcgagcag gcgttgaaca
    10981 cggcgacggc agcttccaca tcgccgagcg ccgccggccg cgtgagatag ccaccttcaa
    11041 gcgttacggc aggtgattca agtatctggg tcgtcatggt tgtgtcctcg tctaaagagt
    11101 cttgcgataa gcgataggcg atacgcgata agccatctgc tatctgccat cagccattcc
    11161 gatgcagctt acccgtccgc ttggcatacc gctcggccca ctggaagatg cgcaagccga
    11221 tcgggatggc gatcacgccc atcaccagca gcggagcgat gtagccccac aggctctcgg
    11281 tcggcgcgcc gttgagcagc gcttcgcgcg acccgtcgag cacatacgtt gcaggcgaca
    11341 gtttcgagaa gacctgcagc cacgccggca gcacgttgac ggggtagtac acgcccgaca
    11401 ccagcagaag catggcgatg atcaggtgcg tcatctgcga tccgcgctcc gggaacagca
    11461 gcggcagcgt cgatccgacg atgctgatgc cgatgaacga caggctgccc gcgatcagca
    11521 gcagaaggaa accccacatg ttcgcatggt cgagccggat ctcgaacagc agcatcgtcg
    11581 ccagcagcac cacacccgtg tgcgccaggc tgtacacgat ggcgaacgcc gtctgtccca
    11641 gcatgtgcat cagccgcgtg atcggcgcca tgagcgtgta ctcgatcgtg ccttcccagc
    11701 gctccagccc gacgacgtcg gtgacccagt agaagatcac ggccagatag cgccagacga
    11761 tggtgccgat ggcgaggtac agcacgagat acttgccgtc cacgccaggc ccgccgatct
    11821 gctccatgcc gaggccgatg tagctcacgg ccaggctgtt ggcgacggtg tacaccagcc
    11881 agactagctc ccacgcccag tagcgcttga cgatattcca gttgcgctcg atgaacgcat
    11941 aacttgcctt aagctcttgc gtaatgaccg acatgtcgaa tctccaatct cagttatcag
    12001 ttgtcagttg tcagttatca gtgaacagtt atccgtgatc actggccact gattactgtt
    12061 cactgatcac tgattgcgga gcggggcttg cgtgttccgt ttggcgctcc cattcttcac
    12121 gtggcaggct catatacacg ccggcgcgcg atcgtcccct gcggctcgcc tcctgcagca
    12181 ttcggccttc gatggcaaag cctgcgctct catacgcacg cagcgcacgg gcgttgtact
    12241 caaacacggc cagcgagatg cggcgcaggt tcagttcgtc gaagccgtag cgcacgatga
    12301 cccgcagcgc gtccgctcct gcaggggtat cccaccgatc gtgatcggcc aggccgatgc
    12361 ccaaccacgc ctcgccgcgc cgccggtcga tgccgaacag gccggcgtga ccgatcgggc
    12421 gatcgccgac gaggggatag atcaggaaac cggcgtgctc gttcgacccc ggctgcatcg
    12481 gtcgaccagt cgccgtgtcc agcaaccgcc acaaggctga catcgccgac cagcgggcga
    12541 gtaactcgat atcactatcc gattgcagcg ccaccaaacg cacgtgatcg cctcgtaaat
    12601 gctgagcaca catattgcga acctcctgac ccgcgtaaaa cgtgacgcgc gacggtgccc
    12661 agcacggttc gcgttctacg ttttactcgt tacgcttttt ctcccactcg tctcgaagaa
    12721 tgcccatgta gatcccgtcc caacgccgac catcgcgaag cacctcctgc ttgctccgac
    12781 cctcgacgac gaagccggcc ttctcgtaag atcggatcgc tcgggcgttg tattcgaaga
    12841 cgccaagcgt cacccgatgg aggttcaact cggcgaacgc gtagcgcagg acgacgcgca
    12901 tggcgtcagt gccgtagccc ttaccccagt actcgcgatc gccaagcccg ataccgaccc
    12961 acgcatccct gtgcgtccag cgaacaccgc ccaggccgac gaagccgatc aagcgatcat
    13021 ctgcgagcgt gcggatcagg aacgggaatc tgtcgtgctt cggttcctcc tcgatctcct
    13081 ccttcacctt cttgaccgac cggggttgcg ccggatcgtc gtcgagcagc cgctgaaact
    13141 cggagtcacg cgaccacgac gcgagcgttt cggcatcttt gtcggaaatc gacgctgtca
    13201 gccgcaccaa ttcgcccttg aaaatctgca tatccatcag cctcacctcg tcagtcatca
    13261 gtagtcagtt atcagtgatc agttatctgt catcagttat atgtagtcag tgacactgct
    13321 cactgttcac tgcccactga ttactgttca ctgattacta gtcactaatc cactcctctc
    13381 gcagcagccc cagcatcagc gcatcccagc gtcggctgtc gcgatagatc gcctgtcgcc
    13441 gccgcacctc gacgacgaat ccgtttcgtt cgaggaagcg cagcgcgcta gggttgtcct
    13501 cgagcgtgac cgcacccagc cgatgcaggt tcagttcgtc gaaggcgtaa cgtaaggaca
    13561 tcttcagtgc ttccgatcca tagcccttgc cgcgatcctc cggccgcccg atgctcaact
    13621 gaatccagcc ggcgctgtgc gtccaatgaa tccagcggat agaggccagc ccgatcagcc
    13681 gatcaccgtc acgcagccgg atggcgaagt tgaactgcgt ccgcttctcc gcctccttat
    13741 ccatctcttc gagtttcttc tttacctggc cgggcgacaa tggccgcgcc ggttcggcat
    13801 ccgtcagccg cagatactcg gggtcgtgcg tccacttcga gatcgtctcg gcgtctttct
    13861 ccgggtcgat cggggccagg cggatacact cgccttcgaa cagttgactg tgcatgtctc
    13921 acatctccgt cgatcgatga atcgcctcgc gtctcacgct tcacgtttca cgtttcacgc
    13981 gtcacgcctc acgctttacg cttcacgcat cccgcctcat cccgcctctt cctcggcgac
    14041 cagttgtttg ccggtcaatt ccaggaacac ttcttccagc gtcggctcgt ggccgttgcg
    14101 cgggatggct cgtttcagct cctgcggtgt gccgagcgcg acgatcttgc cgccgtcgat
    14161 gatggcgatg cggtcgcaca gcgcgtcggc ctcgtacata tcgtgcgtcg tgagcaagat
    14221 cgtcgtgtcg tgcacgtcgc gcagctcgcg gacgaaggct tgcacctcgc gcttcgagcg
    14281 cgggtcgagg ccggtcgtcg gttcgtcgag cagcagcacg atcgggctgc tcaggaaggc
    14341 gcgggcgatg gcgaccttct gctgcatgcc tcggctcatc tcttccatgg gccggtcaat
    14401 cgacctggac tccaggccga gccgggccag gatgtcgcgc acacgggtcc tggcttcact
    14461 cgcgccgacg ccgtacagtc gcgcgccgta cagcaggttc tccatcggcg agagcttctt
    14521 gaagaagctg gcctcgaccg agacgcggtt gatcatcgtc tgcacccggc gcggctctgc
    14581 cacgacatcg tgtccgaaga ccgcgatgcg cccgtcgtca gggacgagca gtgtcgcgat
    14641 caggcggata agcgtcgatt tgccgctgcc gttcgggccg aggatgccga agatctcgcc
    14701 gcgcttcacg tcgagcgaga cgcgattgac cgcgaccttc gcgccttcag ggaggcggcc
    14761 attgccgttc ttgccgttct tcgccgcgaa cagccggctg ccctttccgc cgaaggtctt
    14821 cagcgcgccg tcaatttgca ctgcgatggt gtcgttcatg gctgcttctc ctgttcaggc
    14881 tgcctacaaa cacatggcac accgatttct ctacttgccc atccgtgtaa tccgtaggaa
    14941 atccctgtag aataaacgaa aaggccgtgg caaattggtc acggccttcg cgcgcatcag
    15001 cgcgagaatc gaaattaaaa ggccgtgagc gtcgtacgcg cccacggcct cgctcgactt
    15061 aacgtaactc tacaggcg
//
LOCUS       DBNHXB010000138        12766 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4557661, whole genome shotgun sequence.
ACCESSION   DBNHXB010000138 DBNHXB010000000
VERSION     DBNHXB010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 12766)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12766)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12766
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4557661"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="AC162G_05365"
     CDS             <1..373
                     /locus_tag="AC162G_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5075274.1"
                     /translation="WVVIIVCVLGLLAIIAVALSSRGGDGPCATLDECLGQAEELRAG
                     GDLQGAMERYSRALDFVPGDQRAPNAWIWCDMGELNRRLKRFDDAVSDFERCIQWIEG
                     YPDPQGLRAWAERALRELQPR"
     gene            722..817
                     /locus_tag="AC162G_05370"
                     /pseudo
     CDS             722..817
                     /locus_tag="AC162G_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003590006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            1044..2039
                     /locus_tag="AC162G_05375"
     CDS             1044..2039
                     /locus_tag="AC162G_05375"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5075275.1"
                     /translation="MKIFLTGGTGLIGQPLTHALLRRGWTVVALARNPHSPESQAIQS
                     AGAHLVRGDVTDRESMRTGMSGVDAVIHNAGWYELGITRSAQARMRAINVSGTDNTLG
                     LAVELKIPKIVYTSTIGAFGATADTLADESYERRRPFRSFYEQSKTEAHALATRYQQQ
                     GAPLIIICPSHVIGPGDQSAWGWFVRMYVRRILPPFGFARNTKLSCVSVEDVAEAIAL
                     AVEKGQLGHTYITSGEEKTMEEIIGIWAKTPGGSKPLVWLPYPIAMAVGIIAEPFLRL
                     IGLPAFLSRESIESAYENWLYTAAKAERELGAKFRNAEQLWQETLAAERALARRK"
     gene            2207..2713
                     /locus_tag="AC162G_05380"
     CDS             2207..2713
                     /locus_tag="AC162G_05380"
                     /inference="COORDINATES: protein motif:HMM:NF016001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR5075276.1"
                     /translation="MTSKNYSPFHSLVQKVLSTRFGAWYGSLTAHHFDRMVLKLTGGR
                     MTMGNIVSGLPVAVVTTIGAKSGLSRTLPLLFIRDEHDPNVFALIASNWGSKHHPAWY
                     FNLKANPHATCSIRGQVREYVAHEASGEEYDRFWQYAMGTYLGFPLYKQRAGNRHIPV
                     MVMTPVEQ"
     gene            complement(2812..2994)
                     /locus_tag="AC162G_05385"
     CDS             complement(2812..2994)
                     /locus_tag="AC162G_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075277.1"
                     /translation="MVITVTVPGRAYVPPAIHTWSLGAATSMARWIVAHGAALLAQSV
                     LSFPAGGRWLRHTSSS"
     gene            2984..4267
                     /locus_tag="AC162G_05390"
     CDS             2984..4267
                     /locus_tag="AC162G_05390"
                     /inference="COORDINATES: protein motif:HMM:NF016906.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR5075278.1"
                     /translation="MITKELRLIGGFDPNFTGSDPDLYSTVLDAQWAGSVISLTDAGE
                     VLLQHLALTHGDGTGNCDAGGGGGGVYAAGGTMLRMEGCLIADNVGTTVDQGLGGGLY
                     LRNGLAEIRRSRIVSNSASTASAGDGYGGGIYLYNAMASLVENQILDNVASAARTGSG
                     GGVYFYDVSPGEVLGNAVRGNRAGTGAWGSDGGGLWLGYSSGLYAAGNRIEGNWTNPN
                     QAGYGGGVYVVESDAHLTANTIVSNVTGAGGWVRPGGGVMIGSSRPVTLSNNLIAGND
                     AGSYGGGVHVSMYMPAIASRAILVNNTIAGNGGSGVVTQWHAALTLTNNIIAGHGTGL
                     AAYTAFTGSVYADTNLFWNTADPITGANAIQQDPLLTPDYRVPPNSPAVDAGLTIPWL
                     ATDLAGYPRPQRGRYDLGAFEVLVSYLPLVARTVP"
     gene            4578..5462
                     /locus_tag="AC162G_05395"
     CDS             4578..5462
                     /locus_tag="AC162G_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HIR5075279.1"
                     /translation="MLSGRITPLAPPPRRVPWAVVCSAMLGTLGVVGAGIFVFSMPFV
                     LIFAGELHLIDEIRLSNSGTTAVARVTAVEDTNSTENDVPVYAYRFEFRTRAEELVTA
                     RSFATGRRYSVEDRATVYYLPDKPGVARLENTRRSEFPAFAGLMVLIFPLTGLALFAI
                     GALRGWRDVTLLRHGQVAGARWLRSQMTNMSVNNQPVLKYTYEFQAEDGQTYTGSRRA
                     LPSPHVGDEAHEPVLYLPSHPARSLPLDALSLRYPVRVDDSGQWSNVHSPWSIVWCSL
                     AYTLVFAGILFILTHVIG"
     gene            5697..7373
                     /locus_tag="AC162G_05400"
     CDS             5697..7373
                     /locus_tag="AC162G_05400"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004467 - long-chain fatty acid-CoA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HIR5075280.1"
                     /translation="MNEKPWLKHYDPGVPHTLQPYPERTLLDVVRDTARQRPGHPALL
                     FKGARLLYGELERLSDAFAAALIALGVKQGDRAALLLPNCPQAVIGQLGAWGAGAIVA
                     PLNPLYTARELEQALNECGAETAVVLTPFYQKVKALQPRTQLRRVIATNIKEHLPSAL
                     RLLFTLLKEKKEGHRIRLQSGDLWFSDLLRQHAGSPRPDVTVNPDDPALLLFTGGTTG
                     TPKAAVGTHRALLIAATQLHAWASSLIVDWDDTGLLLMPLFHVYGNVGILGTALVGHY
                     PLAVIPNPRDLNDLLATIRKVRPAVLYGVPTLFIAVLNHPDVQAGKVDFKSIKVCASG
                     AAPLLAETKQRFEALTGGRIMEAYGLTESMLGAAITPLLGTYKEGAAGIPLPHVEMRI
                     ADADTGEGSLPPGEVGEVLIRAPQLMQGYWQRPTETADAIRDGWLYTGDLGYMDEDGY
                     LFIVDRKKDVIKPGGFQVWPREVEEVIASHPAVAEVGVAGVPDAYQGEAVKAWIVLRA
                     GQQATADDIRAYCREKLAAYKVPKHVEFRDSLPKTMVGKVLRRMLVEAES"
     gene            7390..8778
                     /locus_tag="AC162G_05405"
     CDS             7390..8778
                     /locus_tag="AC162G_05405"
                     /inference="COORDINATES: protein motif:HMM:NF018434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5075281.1"
                     /translation="MRRFLFTTLASNDLGLLTRSLPIARELAKRGHRVAFCNPAEAPS
                     KLIAEAGFENLLAKHPLYYLNYLKTKGDLTLKGLYRAIRSGQLKQDYEGLFGFLRQLV
                     RCLPTALPTTSEVWSVDHLAAIGGMLGENFVRAECEALLALMTDYEADVIVDFFNAPA
                     CIAARAVGKPLVTVIQADMHPDSRGFIWWREPPPDLPTPTPTLNKILADYRLPPICKT
                     EELFVGDLTLVLGMPETDPLPAKAQVTYIGPILWQKAEAELPDWFDKSSTEKPVIWVY
                     SGNPRYLPVRTPVDSVVVLHACIAALANAEAQVVLTTGHHALPKDVLPLPANFRYAPY
                     VPGLAMAQQSDLLIHHGGYGSCQLGLYTGTPAVIIPTYSERESNARRVAAVGAGEFVL
                     PTQGAWGKKSVRVEELRAKVKQVLSDPGYAASTKRIAEKMRTYGGAAEAARLIEDLSN
                     STELQREEAARG"
     gene            8934..10025
                     /locus_tag="AC162G_05410"
     CDS             8934..10025
                     /locus_tag="AC162G_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075282.1"
                     /translation="MEWEVVGGFGVELNGQPVERSGQKQVCPAETMTYLLSMDTGVSI
                     ERRAVEIVVAGGGPPPEPTLPSAAAPPSGFDPTTLKPFTAGELYLGQYETGLYPGASN
                     AMLEAHGVAGEQIAATIRPLDTSGQPDDQQGRILALVMGHSNAHMYFEAFRAHLSERA
                     AELNPRFEMLNAAVGGNQLPEIAQLQGDVWNWAQELTTQPGYSSLQVQVLFLHTTYHG
                     CCNDAGLPPGEFPASMQAMQRDLAKVLGYAIQVYPNIKIAYLTSDGFRHFRGFEPHVW
                     REAFAFKWLIESQIEGEPGTVYTGENRQMPWLEWGPYIWDNTWDESYFTDGVHPSPKA
                     LAIFVQKYWGHLSQDSVARPWLFKSGSNR"
     gene            10022..11947
                     /locus_tag="AC162G_05415"
     CDS             10022..11947
                     /locus_tag="AC162G_05415"
                     /inference="COORDINATES: protein motif:HMM:NF014312.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HIR5075283.1"
                     /translation="MKRFTLLVFAVISILALLTRLPSAASPNTPGRYASSAIAMTADG
                     GTLITVNPDSNSVTLVDTASRSVVAELSVGVDPRSVAVSPDGATAYVANQGSDEISAV
                     DLAARVVVTSVAVGDRPIGIAASPNGRFLAVAELGSDHVRILDARTLATLSRRAVPDR
                     PHGLAFTPDSRRLLITHLLSGDVTVLTIQPFALYLPVILKTASTPYREASPWNTPRSL
                     SHPSPITLHSSLLTISTWPHVGPAPAVRVNAAGTRAYLPQTMANGLGLNTQFDTSVFP
                     KVSVLNLATNSHQTSEHISLPEKDQPVGLPWDAALARNDTELWIVNAASNDVSVLGIA
                     TPTLPIRTAHIPVGDNPRGIVISPDGSTAYVNNTLAGTVSVIDANTYTVTSVITVTNI
                     PLPPTLLRGKRLFFSSARSDLARARWIGCNTCHVEGEQDGRTWLLQYVGGVPPGATPI
                     ITRNTTSLLGMIETYPLRWSAEWDESADSEFSVRFEQFGTGLISGTMHPTLGPPNQGR
                     SDDLDSLAAYLDGLTVLTRTHSLSASEQRGQAIFESAETGCASCHPAPLYTDLQQHDV
                     GTADAYGEWFGPLIDTPTLRFLYDSAPYLHDGSAATLRDVLTIKNPAGEHGVTSHLTE
                     EQLDDLIAFLLTLPFEP"
     gene            12064..>12766
                     /locus_tag="AC162G_05420"
     CDS             12064..>12766
                     /locus_tag="AC162G_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR5075284.1"
                     /translation="MNTTRASAALTTATVSGTIRHNGSPVADVNISVLWNGGSQDVTT
                     GPSGAYSVSGVPMGSWLQVFVRPPIALRLAFRNWGAEQVTGNLTKDFDLGSGYRLQGQ
                     FYRPDGMPYTQSFWLETNPVNLVPPDGEWIGVTASNGQFDLVVPSDLFVLNPVPEPLP
                     YHVPRAKSDLRTADQTGVVVTLLSERPAPFSTTPPRASLISVSAPDVDGNATVIGGAG
                     SVPPLAAVVIANLRAS"
BASE COUNT         2468 a         3972 c         3915 g         2411 t
ORIGIN      
        1 atgggttgtt atcatcgtct gcgtgctggg tctgcttgct atcatcgcag ttgccttgtc
       61 ctcccgtgga ggcgatgggc catgtgcgac gctcgacgag tgcctggggc aggcggaaga
      121 actccgggcg gggggtgacc tgcagggcgc gatggaacgc tacagccgcg cgctggattt
      181 cgtcccgggg gaccagcgtg cccccaacgc ctggatatgg tgcgacatgg gggagctcaa
      241 tcggcggctc aaacgctttg acgatgccgt ctctgacttt gaacgctgca tccagtggat
      301 cgagggctac cctgaccctc aggggctgcg cgcctgggcc gaacgagcgc tccgggaatt
      361 acagcccagg tagcggcctg gagcgtgcga agggtcttgc atgtccgcct ccatcgagta
      421 tgcgtccttt ctcgtccggc tgtggcgcca ggtgggcctc gacccgtccg ggtcgagcgc
      481 cgactggcgc tgtaaggccg agcacatcca gagcggacag cactggacct tcaacacttt
      541 cgatgaacta cctggcttcc tccgccgcca ggcagcagaa ccattgacat tctgccggaa
      601 gcccgcgccc ccggaatcga gcagaagacg gtgtcgccaa accggtggag gtcgtcgccg
      661 tctttgggcc gaatgaggag ttccgggcgg aaggtgaggt gggctttttg gtgctcgaac
      721 cttcgctgcc gcgctaatcg cgttgggggt caagaaaggg gatcgcgtgg cactgctgtt
      781 gcccaacagc ctgcagggcg tcatcggcca gctggcccag ccgactttca gttcaagtaa
      841 gtgggctgcc tggtagacgg gctggggaac gcgggtgtga gttgctggcc acgctgacgc
      901 actttggaca gccaaggttg aaggggcagg ttctcttgcc ccttcaacca gacctgtagc
      961 cgcgaggtag agaagtattg ggaacaccga tgctcaatac cgagttgggc gacaaaaggt
     1021 tccagtctcc agggagcagc aacatgaaaa tctttctaac cggtggcaca ggtttaatcg
     1081 gacagccact cactcacgcc ttactcaggc gcggctggac tgtggttgca ctcgcgcgca
     1141 acccgcacag tccagaatca caggccatac aatccgccgg agcacacctc gtccgcggag
     1201 acgtcaccga ccgagagtcg atgcggactg gcatgtcggg cgtcgatgcc gtcatccaca
     1261 atgctggctg gtatgaactc ggcatcacca gatccgcgca agccaggatg cgcgcaatca
     1321 atgtgagcgg caccgacaac acacttggac tggcggtcga gctcaagatt cctaagatag
     1381 tctatacctc caccatcggc gcgtttggcg caacagccga caccctggct gacgagtcct
     1441 atgagcgccg caggccattt cggtccttct atgagcaaag taagacagaa gcccatgctt
     1501 tggctacccg ttaccagcaa cagggcgcgc cactcatcat catttgccca agccatgtaa
     1561 tcgggccggg cgatcagtcg gcgtggggct ggttcgttcg catgtacgtg cgcaggatct
     1621 tgcctccatt cgggttcgca agaaacacga aactaagttg tgtgtctgtg gaggatgtcg
     1681 ccgaagcaat tgctctcgcc gtggaaaaag ggcaactggg acacacctat atcacgagcg
     1741 gtgaggagaa gaccatggag gagatcatag gcatctgggc aaagacgccc ggcgggtcta
     1801 aaccgcttgt ttggctgccc taccccatag cgatggccgt cggcattatc gcggagccat
     1861 tcctgcgcct gataggcttg ccagccttct tatcgcggga atcgatagag tcggcgtacg
     1921 agaactggtt gtacacggcg gctaaggccg aacgagagtt gggggcgaag ttccgcaacg
     1981 ccgaacaatt gtggcaagaa acgctggcag ccgagagggc gttagccagg aggaagtgag
     2041 caccacgtca acctttcata ctggcaactc ttcgccgccc aacccgcgcc tgggcggcta
     2101 atgcacagcc aaagcaactt acaagccaca gggtgaagtt gagcatttca gctgctctgg
     2161 gctgaacgta tctgccgtca gcgtctgcga agacaggagg ccaaagatga ctagcaagaa
     2221 ctactccccc tttcactcac ttgtgcagaa agtgctgtca actcgcttcg gagcttggta
     2281 tggctccctg acggcacacc attttgaccg catggttctc aaactaactg gtgggcgtat
     2341 gaccatgggt aatatagtga gcgggttgcc cgtcgccgtc gtaacaacca ttggcgccaa
     2401 aagcggatta tcgcgcacac tgccgttgtt gttcattcgc gacgagcacg accccaatgt
     2461 attcgcgctc attgcttcaa attggggatc aaaacaccat ccagcctggt atttcaattt
     2521 gaaggccaat cctcacgcca catgctcgat acggggtcaa gtccgagagt atgtcgcgca
     2581 tgaggccagc ggtgaagaat acgatagatt ctggcagtat gcgatgggga catatctcgg
     2641 tttccccctt tataagcagc gtgctggcaa taggcacatc cccgtcatgg tgatgacacc
     2701 cgttgaacag taactctgtt tttgggatgc acaaagcaag tctaccgccc aaccgcgtgc
     2761 agccaccgtt gggccactga ctcacaaaag caaataatga aactgaaacc atcaactgct
     2821 gctcgtgtgg cgcagccagc ggccaccggc cgggaacgat agcaccgact gtgccaataa
     2881 agctgccccg tgcgccacga tccagcgcgc catcgacgtc gcggccccca acgaccaagt
     2941 gtggatcgcc ggcggcacgt acgcccgccc cggcacggta acggtgatca ccaaagaact
     3001 gcgcctcatc ggcggcttcg atccgaactt taccggttct gacccggatt tgtactccac
     3061 ggtgctggac gcgcagtggg caggctcggt catcagcctg accgacgccg gcgaggtgtt
     3121 gctgcagcac ctggcgctca cccacgggga tggcacgggc aactgcgatg cgggcggcgg
     3181 cggcggcggg gtgtatgccg ctggcggcac catgctgcgc atggagggct gcctgatcgc
     3241 cgacaacgtc ggcaccaccg tcgaccaggg cctgggcggc ggcctctacc tgcgcaacgg
     3301 cctggccgag atcagacgca gccgcatcgt cagcaacagc gccagcacag cctcggccgg
     3361 cgacggctat ggcggcggca tctatctcta taacgccatg gcgtctttag tagagaatca
     3421 gatcctggac aacgtggcca gcgcggcgcg caccggcagc ggcggcggtg tgtacttcta
     3481 cgacgtatcg ccgggcgaag tgctgggcaa cgccgtccgg ggcaaccggg cgggcaccgg
     3541 cgcctggggc agcgacgggg gtggcctatg gctgggatac tcgtccggtc tatatgcggc
     3601 gggcaaccgc atcgagggca actggacgaa ccccaaccag gccggctacg gcggcggggt
     3661 ctacgtggtg gaaagcgacg ctcacctgac ggcaaacacc atcgtgagca atgtcaccgg
     3721 cgcaggagga tgggtccggc ccgggggcgg cgtgatgatc ggcagcagcc ggccggtcac
     3781 gttgagcaac aacctgattg ccggcaacga cgccggcagc tatggcggcg gcgtgcacgt
     3841 ctcgatgtac atgccggcca tagccagccg ggccatcctg gtcaataaca ccatcgccgg
     3901 caacgggggc agcggcgtgg tcacgcagtg gcatgccgcc ctcaccttga ccaataacat
     3961 catcgccggg cacgggacgg gcctggccgc ttacaccgcc ttcaccggca gcgtgtatgc
     4021 cgataccaac ctgttctgga atacggcgga tccgatcacc ggcgccaatg ccatccagca
     4081 ggacccgctg ctgacgcccg attatcgcgt gcctccgaac tccccggcgg tcgacgcggg
     4141 gctcaccatt ccctggctgg cgacggacct ggcgggctat ccccggccgc agcgcggccg
     4201 ctacgacctc ggggcgtttg aggtgctggt gagctatctg ccgctggtcg cgcgcactgt
     4261 cccgtagtga ctttaacctg aggtctgtca tcggaccgca gtgtttgtca gaggaatggt
     4321 catgcacctt acccgttcgc caacattggc attgctgggc gggcggtgcc cagctatctg
     4381 cagacacttt gctttcgccg gatgtgggtg taaacttgcc ggactttggc agaattccgg
     4441 ccccgagccc ctagagcggt ttccgattgc ttgtacgccg ctccggaatc gagccttagt
     4501 ccagcaggct taatgtctgg gagctacgca gttgaactgc agtcttcaag cgggcaggcg
     4561 caggaggtag gacagcgatg ttatccggac gcatcacgcc actcgcaccg ccgccgcgcc
     4621 gggtgccgtg ggccgtcgta tgctcggcca tgctgggcac gctgggtgtc gtaggcgcgg
     4681 gcatattcgt cttcagcatg cccttcgtgc tgatctttgc cggtgaactg catctgatcg
     4741 acgagatacg cctgtctaac tccggcacga cggctgtggc gagggtgacg gccgtcgaag
     4801 acacaaactc gactgagaat gacgttccgg tttacgcgta ccgtttcgag ttcaggacgc
     4861 gcgccgagga gctcgtcacg gcgcgcagct tcgcgaccgg ccggcgatac tccgtcgagg
     4921 acagggcgac ggtctactac ctgccggaca agcccggcgt cgctcgtctg gagaacacgc
     4981 gccgctccga gttccctgca tttgcgggtc tgatggtgct gatcttcccc ctgaccggac
     5041 tggcgttgtt cgctatcggc gcactgcgcg gatggcgcga tgtcacgttg ctgcgccacg
     5101 gccaggtcgc cggcgcgcgc tggctgcgga gccagatgac gaacatgagc gtcaacaacc
     5161 aacccgtctt gaagtacacc tacgagtttc aggctgaaga cggacagaca tacaccggtt
     5221 cgcgacgggc gctgccctcg cctcatgtcg gcgacgaggc acacgaaccg gtgctgtatc
     5281 tgccgtccca cccggcccgc tcgctgccgc tggatgcgct gtcgctgcgc taccctgtcc
     5341 gcgtcgacga ctctggccaa tggagcaacg tgcacagccc gtggtcgatc gtctggtgca
     5401 gcctggccta cacgttagtg ttcgcgggta tcctgtttat cctcacgcat gtcattggat
     5461 agccctcatg cttcagcgcg tgcgcgatac ttacgcgata tggatcaagg ctggtggtga
     5521 tcccgggtgc aggccacacg tcaacggtgg aggaaccaga agcggtaaac gcggccctgg
     5581 tagagtttct cagtgaccaa gcaggctaga tccggctggc tttatcgagc gctgactgtc
     5641 ccggagcttg agcaccggca ccgtcacctg ttgcccgaga gcggaggttt gacaagatga
     5701 acgagaaacc ctggctcaag cactacgacc ccggggtgcc gcacaccctg cagccgtacc
     5761 ccgagcgcac gctgctggat gtggtgagag acacggcgcg ccaacgaccg ggccacccgg
     5821 ccctgctttt caagggcgcg cgcctgttgt acggcgaact cgagcggttg agcgatgcct
     5881 tcgctgccgc gctgatcgcg ttgggagtca agcaagggga tcgcgcggcg ctgctgttgc
     5941 ccaattgccc gcaggccgtc atcggccagt tgggtgcgtg gggggccggc gccatcgtcg
     6001 cgccgcttaa tccgctctat accgcgcgcg agttggagca ggccctgaac gagtgcggcg
     6061 ccgagacggc cgtcgtgctc acccctttct accagaaggt caaagccctg cagccccgta
     6121 cccagttgcg ccgcgtgatc gctacgaaca tcaaagagca cttgccgtcg gctctgcgct
     6181 tgctgttcac actgctcaag gagaagaagg agggccatcg catcaggctt cagtcgggcg
     6241 acctctggtt cagcgacctg ctgcgccagc acgccggctc gcctcggcca gacgtaacgg
     6301 ttaatcccga cgatccggcg ctgctcctgt tcaccggcgg cacgaccggg acgcccaagg
     6361 cggcggtagg cacgcaccgc gcgttgctga tcgccgcgac gcagttgcat gcctgggcca
     6421 gcagcctcat cgttgattgg gatgacaccg gtctgctgct catgcccttg ttccatgtgt
     6481 acggcaacgt cggcattctg ggtacggcgt tggtggggca ctatccgctg gccgttatcc
     6541 ccaatccccg cgacttgaac gacctgctcg ccacgattcg caaggtccgc cctgcggtcc
     6601 tgtacggcgt gccgacgctg ttcatcgcgg tgctcaacca tcctgatgtt caggctggca
     6661 aggtggattt caagtcaatc aaggtgtgcg cttcgggagc cgcgcccttg ttggccgaga
     6721 ccaaacagcg cttcgaggcg ctcactggcg gtcgcatcat ggaagcctat ggcctcacgg
     6781 agtccatgct cggtgcggcc atcaccccac tgctgggcac ttataaagaa ggggcggctg
     6841 gaatcccgct gccgcacgtc gagatgcgca ttgccgatgc cgacaccggc gagggcagtc
     6901 ttcccccagg cgaggtcggc gaggttctga tccgcgcccc gcaactgatg cagggctact
     6961 ggcagcgccc caccgaaacg gccgatgcta tccgcgacgg ctggctgtac accggcgacc
     7021 tgggctatat ggatgaagat ggatacctgt tcatcgtgga tcgcaagaaa gacgtaatca
     7081 agcccggtgg cttccaggtc tggccgcgcg aggtggaaga ggtcatcgcc tcgcacccgg
     7141 cggtggccga ggtcggtgtg gcgggcgtgc cggatgccta tcagggtgag gcggtcaagg
     7201 cgtggattgt gttacgtgcg ggccaacaag ctactgccga cgacatccgc gcctattgcc
     7261 gtgagaaact ggccgcctac aaagtgccca agcacgtcga gttccgcgac agcctgccca
     7321 agaccatggt tggcaaggtg ctgcggcgga tgctggtaga ggctgagagc tgaaggctct
     7381 gctgacgcta tgcggcgatt tctgttcacc accctcgctt ccaacgatct cggcctgctg
     7441 acccgatcct tgccgatcgc ccgcgagttg gcgaagcgag gccaccgcgt cgccttttgc
     7501 aacccggctg aagcgcccag caaactgatc gcggaagcag ggtttgaaaa ccttctcgcc
     7561 aaacatcccc tgtactatct caactatctc aagaccaagg gcgacctgac tctcaagggc
     7621 ctctaccggg caatcagatc gggacagttg aagcaggact atgagggtct cttcggcttc
     7681 ttgcgccaac tcgttcgatg cctccccact gccttgccaa ccacttctga ggtttggagc
     7741 gtggatcacc tggctgcgat tggcgggatg ctcggcgaaa acttcgtccg cgccgagtgc
     7801 gaagcgctcc tcgcactgat gacagactat gaggcagatg tcatcgtaga tttcttcaac
     7861 gcgccggcct gtattgcggc ccgggcggtt ggcaagcctt tggtgacggt catccaggcc
     7921 gacatgcacc cggacagccg ggggttcatc tggtggagag agccgccgcc ggatttacct
     7981 actcccaccc cgaccttgaa caagatactt gcagactatc gcctgccgcc gatttgcaag
     8041 accgaagaac tcttcgtggg tgatctgacc ctggtattgg gaatgccgga aacagacccc
     8101 ctgccggcga aggctcaagt cacctacatc ggtccaatcc tgtggcagaa ggccgaggca
     8161 gaactgccgg actggttcga caagtcgagc acagagaagc cagtcatttg ggtgtattcg
     8221 ggcaatccgc gctatctacc cgtccgtacg ccagttgatt cggtggtcgt cctgcacgcc
     8281 tgcatcgccg cgctcgcaaa tgcggaggcg caggtcgttc tgaccaccgg gcatcacgct
     8341 ctgccgaagg acgttctgcc cttgccggcc aatttccgct acgcgcccta cgtcccgggc
     8401 ctcgcgatgg cacaacagag cgatttgctg atccaccacg gcgggtatgg ctcgtgccaa
     8461 ctcggtctct acaccgggac tccggcggtc atcatcccaa cttactccga gcgcgaaagc
     8521 aacgcccgac gggtcgccgc cgtaggagcg ggagagttcg tcttgcccac ccaaggcgcc
     8581 tggggaaaga agtcggtgcg cgtggaagaa ctgcgcgcga aggtaaagca ggttctgtcc
     8641 gatcctggat atgccgcgag taccaaacga atcgccgaga agatgcggac ttacgggggc
     8701 gcagcagaag ccgcccgcct gattgaagac ttgagcaact ctaccgaact tcagcgggaa
     8761 gaagccgcgc gcggatgaca cagattctca ttgcgagtct ttttcttttc gtgcttgtgg
     8821 gttgcggcac accgactccg ccagagtctg tgaagggtga cgggctacct gccgaggtgc
     8881 aaatatcaat tgaaaccgat aaggatacgc tgagacccgg cgaatgcgcc actttggaat
     8941 gggaggtggt cggcggcttc ggcgtggaac tgaacgggca gccggtcgaa cgctcgggtc
     9001 agaaacaggt gtgccctgcc gagacgatga cttacctgct gagcatggac accggcgtga
     9061 gcatcgagcg gcgcgcggtc gagatcgtcg tggcaggcgg ggggccgccg ccggagccga
     9121 cgctccccag tgctgccgct ccgccatcag gcttcgaccc gaccacgctg aagccattca
     9181 ccgccggcga gctctatctc ggccagtacg aaaccgggct ttaccccggc gcatccaacg
     9241 cgatgctcga agcccatggc gtggcaggcg agcagatcgc cgccacaatc cggccgctcg
     9301 acaccagtgg ccaacctgac gaccagcagg ggcgcatcct ggcgctggtg atggggcatt
     9361 ccaatgctca tatgtacttt gaagcgttcc gcgcgcacct gagcgagcgg gcggcagaac
     9421 tgaatccccg tttcgagatg ctcaacgcgg cagtgggtgg gaaccagttg ccagagattg
     9481 cccagctcca gggggacgtg tggaactggg cgcaggaatt gaccactcag ccgggctatt
     9541 cgtcgctgca ggtgcaagtg ctcttcttgc acaccaccta tcacggctgc tgcaacgacg
     9601 caggcttgcc cccaggcgag tttcccgcct caatgcaggc gatgcaacgc gatctggcga
     9661 aggtattggg atacgccatc caggtctatc ccaatatcaa gatcgcctat ctcacctcgg
     9721 atggttttcg gcacttccga ggattcgagc cgcacgtctg gcgcgaggcg ttcgccttca
     9781 agtggctgat cgagagccag atcgagggag agccgggcac agtctacacg ggtgaaaacc
     9841 gccagatgcc gtggctggaa tggggaccct acatctggga caacacctgg gatgaaagtt
     9901 attttactga tggcgttcac ccatctccca aggcattggc gatattcgtg cagaagtatt
     9961 gggggcatct gagccaggac agcgtggcca ggccctggct gttcaaatcc ggatcgaata
    10021 gatgaaacgc ttcacgctat tggtgtttgc ggtgatctcg atcctggcgc tgctcacccg
    10081 gttgccgagc gccgcctcac cgaatacacc cggcagatat gcgagttctg cgatcgccat
    10141 gacagccgat ggcggcacgc tcatcaccgt caaccctgac tccaattcgg tgacactggt
    10201 cgatacggca agtcgatcgg tcgtcgcgga actctcggtc ggagtcgatc cgcgcagcgt
    10261 ggccgtttcg cccgacggtg caaccgctta cgtggccaat caaggctcag acgagatctc
    10321 ggccgttgac ctggccgcgc gagtggtggt gacgagcgtg gccgttggcg atcgcccgat
    10381 agggattgcc gcttcaccga acgggcgctt cctcgccgtc gccgagctag gcagcgacca
    10441 tgtgcgtatt ctggatgcac gcacgttagc cacgctatcg cgccgcgccg ttcccgaccg
    10501 gcctcacggc ttagccttca cgcctgacag tcgtcgcctc ctgatcaccc acctcctcag
    10561 cggcgacgtc accgtcctga ctatccaacc gttcgcgctt tacttgcctg taattctcaa
    10621 gactgcgtca acgccatatc gtgaagcatc tccgtggaac accccacgca gcctatcaca
    10681 cccatcaccc atcacgcttc actcgtcact tctcaccatc tccacctggc cgcacgttgg
    10741 gccggcgccg gccgtcaggg tcaacgccgc cggaactcgc gcctatctgc cgcaaacgat
    10801 ggcgaacggc ctgggcttga acacacaatt tgatacgagt gtctttccca aagtatcggt
    10861 gctgaacttg gcgacgaaca gccaccagac ttcggaacac atttcattgc cagaaaaaga
    10921 tcagccggtc ggcttgccgt gggatgcggc gctggcccgc aacgataccg agctatggat
    10981 tgtcaacgcg gccagcaacg atgtctcggt attaggcatc gctacgccga cgctgccgat
    11041 tcgcacagca catattccgg tcggcgacaa cccgcgcggc atcgtgatca gccccgacgg
    11101 cagcacggcc tacgtcaaca acaccctggc cggaacggtg agcgtgatcg atgcgaacac
    11161 ctataccgtc accagcgtca tcaccgtgac gaacattcca ctaccgccca cactgctgcg
    11221 cggcaagcga ttgttcttct ccagcgcgcg atccgatctg gcgcgtgccc gctggatcgg
    11281 ttgcaacacg tgtcacgtcg aaggcgaaca ggatgggcga acgtggctgc tgcaatacgt
    11341 cggcggcgtg ccaccgggcg caacgccgat catcacgcgc aacacgacga gcctgctggg
    11401 catgatcgaa acgtatccgc tgcgctggtc ggccgagtgg gacgaatcgg ccgatagtga
    11461 atttagcgtt cgcttcgagc aattcggtac cggcttgatt tcagggacga tgcatcccac
    11521 acttggccca cccaaccaag gccgctcaga cgatttggat agcctggcgg catacctcga
    11581 cggcctgact gtgctcacgc gaacgcacag cctgtctgcg tctgagcagc gcggccaggc
    11641 gatcttcgaa tcggcggaga caggctgcgc gagttgtcat ccggcgcccc tttacaccga
    11701 cctgcagcaa catgatgtcg gcaccgccga tgcgtacggc gagtggttcg gcccgctgat
    11761 cgatacgccc acgctgcgct ttctgtacga cagtgcaccg tatctgcacg atggcagcgc
    11821 tgccactctg cgcgacgtgc tgacgatcaa gaatcccgcc ggcgagcacg gcgtcacctc
    11881 gcatttgacc gaagagcaac tcgacgactt gatcgcgttt ctgctgactc tgccatttga
    11941 accgtagagg aggtacacat gaagaccagg ttaggagtca tcgttcttgc cggtggattg
    12001 gcatttttac tggcctgctt gttggcatcg actctgttgg gcacacgtca gccggcggaa
    12061 ttattgaaca ccacccgtgc ttcagccgcg ctaaccaccg ccaccgtctc cggcacgatc
    12121 cgccacaacg gcagcccggt cgcagacgtc aacatcagcg ttttgtggaa cggcggcagt
    12181 caagacgtga ccacgggtcc gagtggagcg tatagtgtca gcggcgtgcc gatgggtagt
    12241 tggctccagg tttttgtacg cccaccgatc gcgctgcggc tggcctttcg caattggggc
    12301 gccgagcagg tgaccggcaa tctgaccaag gactttgatc tgggaagcgg ttatcgcctt
    12361 cagggccaat tctaccggcc ggatggcatg ccctacacac agtcattttg gctggagacc
    12421 aatccggtca atcttgttcc accagacggc gaatggatcg gtgtgacagc atccaacggc
    12481 cagtttgatc tggttgtgcc atctgatctg ttcgtcctga atcccgttcc tgaaccgctg
    12541 ccctatcacg tgccacgtgc gaagtccgat ctccggactg cggatcagac tggcgtcgtg
    12601 gttacactgt tgagcgaacg cccagcgcca ttctcgacga cgccgccgcg cgcgagcctg
    12661 atcagcgtca gcgccccaga tgtcgacggc aacgcgacgg tgatcggcgg ggcgggcagc
    12721 gtgccaccgc tggccgctgt cgtcatcgcg aacttgcgcg ccagca
//
LOCUS       DBNHXB010000139        10054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1035318, whole genome shotgun sequence.
ACCESSION   DBNHXB010000139 DBNHXB010000000
VERSION     DBNHXB010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10054
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1035318"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1948
                     /locus_tag="AC162G_05425"
     CDS             <1..1948
                     /locus_tag="AC162G_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR5075285.1"
                     /translation="FPWVRTIHILRDGRDVALSTLEWAREDKGPRKFELWGEEPVAVC
                     ALWWRWHVRTGRQGGAALRPTQYCEVRYEDLVTQPEKILYALADFLDLPFAQAMLTYY
                     VGKVRNKPGLSAKKAWLPPTLGLRDWRTQMRERDVELFEALAGDLLTALGYERGVTTI
                     SQDVIAVAERCRQWWESAIARREGSAGQPLEPVVHRVENNTFSGTRIRSTAPPRRASA
                     LSSDSVDLARRDPALPGLATVLDPEAFVGALRRSLPDADLGAARITYVKYKPGTVCLV
                     GYRLTVAGTVVDVYAKAYGVTASEKLQKALEQPGEPGALGRGRIPLDDCQIVVSAFPN
                     DAKVRALPSLRGAETRLRLLRKLFPDRPSLWVGTLQRLVYKPERRYVARLVTEDGAQA
                     VLKLYTQPGYELVQRRRKPYESRPPLRLASRIGKSDRHTALAFEWLPGRLLSEAILDP
                     QFDDHALATVGAAIAQLHAQIPAGLPHLTRETEASTLLAEASMLGLICPPLARLAETL
                     AYRIAAHLVKRSAIYSLIHGDFHAWQVLLNGDTAAILDLDRAVCADPMIDLGLFAAHL
                     EHEVMRGNLPANRVEPLVQALLAGYAAVTRQPVADDEIQLYTAVELLRLAPRFFRYHE
                     SDWPERIAASLDRVETILAPIPVP"
     gene            1974..3059
                     /locus_tag="AC162G_05430"
     CDS             1974..3059
                     /locus_tag="AC162G_05430"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017289947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR5075286.1"
                     /translation="MTEALRIFVTDPFGVADDPAMPFLTQVLDPLTVQRRFEDCLPSL
                     TAGNKPFQLRAIRVLRHKPGRRCLIEYDIERRGAEAITLIGKVRARGPDHTTCHLIES
                     LWNAGFGADSPDGISVPEPVGIIPEFQMWLQYKVPGVSATRLLTESGGTGLARRIAEA
                     IHKLHQTGIPSTRRHTMADELRILHERLSLVARMNPQWVNRLERILDACDRLGATTPE
                     PTFRGIHRDFYADHVVVDGPRLYLVDFDLYCGGDPGLDIGNFVAHLTEQSLRTLGNPM
                     ALRNREQALEERLVELAGEATRVGVRAYTILTLVRHIYLSTQFTERRPFTECLLQMCE
                     QRLGCPNSTLDCDALFDTESRLLQQRA"
     gene            3354..3476
                     /locus_tag="AC162G_05435"
     CDS             3354..3476
                     /locus_tag="AC162G_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5075287.1"
                     /translation="MRRAGQPEDVADVIVFLASAQARWRTGQLVYVGIGWRMHQ"
     gene            3491..4327
                     /locus_tag="AC162G_05440"
     CDS             3491..4327
                     /locus_tag="AC162G_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5075288.1"
                     /translation="MSRYPVYLEIADGGLCMAHVIDLPGCIVRAPSRDAAWRQLPDAI
                     RDYHAWLRHHGEPALPEDESIEIAIAGESIGFGPFDPGDAAALFPPDREPLTSEEMER
                     HFRLMTYSRADLLALVRDPSTLPPSGVAGQILSDEALDWQPDPQSFTIRRVLRHIGNA
                     EEWYVSRLVPPETLPSEWEHDENLPILEFLAMERSTAIARLRRLTEEERSRIFYPTRW
                     TQHPDEPWTARKALRRFLEHEREHAAQVQAILAAWHLRSVSEPKSRQGSDVNHESVPH
                     NE"
     gene            4302..5072
                     /locus_tag="AC162G_05445"
     CDS             4302..5072
                     /locus_tag="AC162G_05445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5075289.1"
                     /translation="MKAYRITSRQDAEKAYPCATESPSPFWADGLPLSRTWFADNLGQ
                     HVEGFHLEDDAGQVIGHIYWAPSDKALVPYRIEDRVAFIYCEWVQRQHRGRGGLRMLF
                     DAFVDFLRADGYKGILVDGTDYEDCMHQRHFARRGFRVIRDTGAGRLMYLPLSQESVQ
                     VEPLSSRITREGTAPVEVRIIGSRFCPVGASAVLALRKAASEFGDRVTVKEMPAGADA
                     LDRYGVADGIFINGQVRFFGPVTEEQVRQAIREAIASV"
     gene            5097..5993
                     /locus_tag="AC162G_05450"
     CDS             5097..5993
                     /locus_tag="AC162G_05450"
                     /inference="COORDINATES: protein motif:HMM:NF046451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5075290.1"
                     /translation="MDNHTLWNLAGEAEDATVAHYEPVFNRFSADSGLDDPTIGLLLA
                     AVTFVPETTSAARLLVRGPYTAAETYLARLAAVTEKGYMTEVSPGEYRLNDLGRAEVQ
                     RLIEEARTVMAQVDPLPLADSQRLAILLDQIVQASLNTPPPPDTWSIRLSFKLMPATS
                     PPLPYIEQAVSCLSGYRDDAHLAAWHPGGLSAAALETLTVFWRGEADSLAAVCQRLSR
                     RGHSPDVYAEALRELRTRGFVKGPEVAPRLTEAGRAFREQVERDTDRYFFAPWACLDE
                     AQRVELAELLTRLRGGLRESRA"
     gene            6068..6712
                     /locus_tag="AC162G_05455"
     CDS             6068..6712
                     /locus_tag="AC162G_05455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HIR5075291.1"
                     /translation="MTIRPASTPSLHISMTGTPLPQAIALPTPQLKGRLTLEEALALR
                     RSVRDFSDTPLTLAELGQLLWAGQGVTHPAGLRTAPSAGALYPLEVYAVMREGVYHYE
                     PETHRLTVQIQGDVRQALHEAALRQEAVLNAPAVIVIAAVYARTTQKYGEERSPRYVH
                     LEAGHAAQNVLLQAVALDLGAVPIGAFWDDQVKQVLALPPDQQPLYLIPVGHPR"
     gene            6758..7522
                     /locus_tag="AC162G_05460"
     CDS             6758..7522
                     /locus_tag="AC162G_05460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR5075292.1"
                     /translation="MSLVPGTDIFLKRSGLSSPANSLLLTLVIAGVALAAREFFQVFD
                     GLFVGSTLISQGIVMWLGLVIVAQMIVRRDSLKARWDERAFSIAFRWCAIPGLTLVAM
                     GIAHFAWIEGARILPREIALIPFVYLLVTGIVLWLRALMVFGMDNLSLMYVYFSSESR
                     LVNSNVYGVLRHPIYSAVIRIAFALILWNGSGFALFAGVFVPLSMTAWLRWVEEPELI
                     ERFGESYRAYRARVPAFFNLNPQTWPVLWRFLVTGK"
     gene            complement(7633..8256)
                     /locus_tag="AC162G_05465"
     CDS             complement(7633..8256)
                     /locus_tag="AC162G_05465"
                     /inference="COORDINATES: protein motif:HMM:NF046921.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoP family protein"
                     /protein_id="HIR5075293.1"
                     /translation="MRALIQGLDRLLRCIYDVYEFCAAPNCLFRLRMSRAPHVLPLPD
                     GEVGAGAPVVELHLWNEHIPRIPDEGPDIAWARRVHRMLIDSFQAVAVHIRHDPRLLN
                     AQAVGGVTVLLTVSDPSGGKSLMRRLGFTVLPYHTPLGRFGEFWENFYTWMLIWTFNT
                     ASLRQRRLLRLRRSEVWMSAEEFLRRYERASGVKHSERVLTADCEAS"
     gene            complement(8277..8564)
                     /locus_tag="AC162G_05470"
     CDS             complement(8277..8564)
                     /locus_tag="AC162G_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075294.1"
                     /translation="MLQGLLVLLIICSWVYWLVALCLVYDFFRRARPLPVRDMLSALV
                     WLAGGFGRRVIWRGEEFVLVHEGRMHPVSPGTVGSEAGHLRGGSRGGTAVT"
     gene            complement(8566..8814)
                     /locus_tag="AC162G_05475"
     CDS             complement(8566..8814)
                     /locus_tag="AC162G_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075295.1"
                     /translation="MIQVLRRLSVPTAELYFHPTTGSGSETLGPNAGDLATLLSPAVR
                     QVIQERGLRLTTYPALHAATQGSSAPLHPPDDTSHKVK"
     gene            complement(8830..9465)
                     /gene="hpnK"
                     /locus_tag="AC162G_05480"
     CDS             complement(8830..9465)
                     /gene="hpnK"
                     /locus_tag="AC162G_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HIR5075296.1"
                     /translation="MWIILNADDFGRSTGINTAVIRAHYEGVLTSASLMVTGDAVEEA
                     ITLARETPTLAVGLHVVVANGRSVLSPGEIPHLVDGDGHFSNDPIRIGLRYYFSRMAR
                     AELTRELAAQFERFTTTGLPLSHVDSHLHLHPHPIVFDLMLPLAVKFGARGVRLPRDE
                     LWLALRHNRQRAATKIMWAIVFRLLCRWCVSRLRNHRPIVAHRVYGLMRTG"
     gene            9590..10009
                     /locus_tag="AC162G_05485"
     CDS             9590..10009
                     /locus_tag="AC162G_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5075297.1"
                     /translation="MDALFRLFGTGGRCRAKLNAKPVRILNAANLRVPLESGAGRWGA
                     RRRGSSELGLPPRCGSGSRLSYPRWGDILYQFLTETLTLSVLGGVIGILASAGVSALV
                     NASGLIDTVVSMESIVLAFGFSAAVGVFFGMCPANYG"
BASE COUNT         1898 a         3068 c         3141 g         1947 t
ORIGIN      
        1 gtttccctgg gtcaggacga tccacatcct ccgtgacgga cgggatgttg cactgtccac
       61 gctggaatgg gcacgcgagg acaaaggacc gagaaaattc gagctgtggg gggaagaacc
      121 tgtagccgtg tgcgcgttgt ggtggcgctg gcacgtgcgc accggtcggc aaggcggtgc
      181 ggctctaagg ccgacgcagt attgtgaggt gaggtatgaa gacctggtaa cccagcctga
      241 gaaaatcctt tacgcccttg ctgacttcct cgacctgcca ttcgctcagg cgatgctaac
      301 ctactacgtc ggcaaggtcc gaaacaagcc tggtctctcg gccaagaagg catggctccc
      361 gccgacccta gggctaaggg attggcgcac tcaaatgagg gaacgagacg tggagctgtt
      421 cgaggcgctc gccggggatt tattgactgc ccttggctat gagcggggcg tcaccaccat
      481 ctcacaagat gtcatagcgg ttgccgagcg gtgtcgccag tggtgggagt cggcgatagc
      541 gcgacgggag gggagcgccg gccagccgtt ggagcctgtt gtgcatcgag tagaaaacaa
      601 cacattcagc ggtacaagaa tccgatcgac cgcgccaccg aggagagcat ctgcgctatc
      661 atctgatagc gtggatctcg cgaggcgcga tcccgctctt ccggggctag ctaccgtgct
      721 tgacccggag gcctttgtcg gtgctctgcg gcggtcattg cccgacgccg atttgggggc
      781 agcccgcatc acctacgtga agtacaaacc cgggacggtt tgcctcgtcg gctaccggct
      841 gacagtggcc gggacggtcg ttgatgttta cgccaaagcc tacggggtca ccgcttcgga
      901 gaagctgcaa aaggcgcttg aacagcccgg cgagcctgga gcgctgggcc gggggcgcat
      961 tcccctggat gattgtcaga tcgtcgtgtc cgctttcccg aatgatgcca aggtgagggc
     1021 gctcccctcc ctgaggggtg ccgagacccg acttcgcctg ctgcgcaagc tttttcccga
     1081 tcgccccagc ctctgggtgg gcacgctgca acgcctggtg tataagcccg agcggcggta
     1141 tgttgcccgg ctggtgaccg aggatggagc ccaggccgtg ctgaaactct acacccagcc
     1201 gggttatgag ctggtgcaac gccgccggaa accctacgaa tcgcgtccgc cgctccggct
     1261 cgcgtcacgg atcggcaagt ctgaccgtca cactgccctg gcgtttgaat ggctgcccgg
     1321 ccgcttattg agcgaggcga tcttggatcc ccagttcgac gaccatgccc tggcaacggt
     1381 cggcgccgcc atcgcccagc ttcacgcaca gattcctgcc ggattgccgc acctgacgcg
     1441 tgaaacagag gcgagcacgc tgctcgccga ggcgtctatg ctcggcctga tctgccctcc
     1501 cctcgcccgg ctggccgaga ccttggccta ccggattgct gctcatctgg taaagagatc
     1561 ggctatttac agtctcatcc acggggactt tcacgcgtgg caagtgctgc tcaatggcga
     1621 cacagcggct atcctcgatc tcgatcgagc cgtctgtgcc gaccccatga tcgacctggg
     1681 gctgttcgcg gcccatctcg agcacgaggt catgcgcggc aatctccccg ccaaccgggt
     1741 agaaccgctg gtgcaggccc tgctggcagg ctacgcggcg gtcacccgtc agcccgttgc
     1801 ggatgacgaa atccaactgt acaccgccgt ggaattgctc cggcttgcac cgcgcttttt
     1861 ccgctaccac gaatccgatt ggcccgagcg gatcgcggcc agccttgacc gagttgagac
     1921 gatccttgct ccgatccctg tgccttagcg caccatgaac tagtcctttg aagatgaccg
     1981 aagcgctgag aatctttgtg accgacccgt tcggcgttgc ggacgacccg gccatgccct
     2041 ttctcaccca ggtcctcgac cctctcacgg tgcagcgtcg atttgaagat tgcctgccat
     2101 ccctgacggc aggaaacaag ccgttccagc tacgtgcgat ccgtgtgctg cggcacaagc
     2161 ccggacggcg ctgcctgata gagtacgaca tcgagaggag aggagccgag gcgatcaccc
     2221 tgatcggcaa ggtgcgggcg cgaggaccgg atcatacaac ctgccatctg atcgagtcgc
     2281 tctggaacgc tggttttgga gcagatagcc cagatgggat tagcgtaccc gagccagttg
     2341 ggatcattcc ggaattccag atgtggctcc aatacaaggt accaggtgta agcgcgaccc
     2401 ggctactgac agaatcgggc gggacagggc tggcacgacg gatcgcggag gcgatccata
     2461 aactccacca aacgggaatc ccatccaccc gccgccacac catggcagat gaactgagaa
     2521 ttctgcatga gcgcctgtcg ctcgtcgctc ggatgaatcc gcagtgggtg aaccgtctgg
     2581 aacgaattct tgacgcgtgc gaccggctcg gcgcaacgac tccagagccg acatttcggg
     2641 gaatccatcg ggatttttac gcagaccacg tcgtcgtgga cgggcctcgt ctgtacctcg
     2701 tcgatttcga tttgtactgc gggggggacc ccggactgga cattggcaac ttcgtggcgc
     2761 acctcacgga acagagtctg cgcaccctgg gcaatccaat ggcactacga aatagggaac
     2821 aggcgctgga agaaaggttg gtggagcttg ccggcgaggc aactcgcgtc ggcgtaaggg
     2881 cttacaccat cctcacactg gtgcgtcaca tctacttgag cacacagttc accgaacgcc
     2941 gcccattcac cgagtgcctg ctgcagatgt gtgagcagcg tcttggctgc cctaattcaa
     3001 cccttgattg tgatgcgctt ttcgacaccg aaagccgcct gcttcagcag cgtgcgtgac
     3061 cagacctggg ttaagcatct ggcaacgcat gtaccccgat ttgtacccgc cacctgtatc
     3121 gcgatacatg gactccccgg ccgcttcggt ttatacttga gacgccatag ccaggcaact
     3181 gaatcatgaa gcgagtccgt cgcgcggcca gcgaacggca tcgaatcgta cagccgctca
     3241 gccgcgtcgg agatgggcca atacggcatc acggtcaaca tcgtcgcccc ggcccgatcc
     3301 agaccggttg catcacacct gaggacgaat cgggcatagc ggcctagaca ccgctgcgcc
     3361 gtgccggcca gccggaggac gtggcagatg tcatcgtctt tctggcttct gcgcaggcgc
     3421 gttggcggac cgggcaactg gtgtatgtcg gcattggctg gcgaatgcac cagtgaatgg
     3481 gtgatgctct atgtctcgct accctgtgta cctggagatc gccgacggtg gcctctgcat
     3541 ggctcatgtc attgacctgc ccggctgcat cgttcgcgca ccgagccgcg acgccgcatg
     3601 gcgacagtta cccgacgcca ttcgcgacta ccacgcctgg ctgcgccacc acggtgaacc
     3661 ggctctgccc gaagacgaat cgatcgagat cgcgatcgcc ggcgagagca tcggctttgg
     3721 ccccttcgac ccgggggatg ccgccgcgct tttcccgccc gaccgtgagc ctctcacctc
     3781 ggaagaaatg gagcgtcact tccgcctcat gacgtacagc cgcgccgatc tgctggccct
     3841 cgtgcgtgac ccttcgacac tccccccttc gggggtcgca gggcagattc tgtctgatga
     3901 ggcgctggac tggcagcccg atccccaatc cttcactatc cgccgtgtgc tgcgccacat
     3961 cggcaatgct gaggagtggt acgtctctcg cctggtgccg cccgagacct tgccatccga
     4021 gtgggagcac gatgagaatt tgcctatcct cgagttcctg gcgatggaac gcagcacggc
     4081 gatagcacgg ctacgacgat taaccgaaga agaacgatcg cgtatctttt atcctacccg
     4141 ctggacgcag catcccgacg agccgtggac cgcgcgtaag gcgctgcgcc ggttcctgga
     4201 acacgagcgg gagcatgccg cacaggtgca ggcgattctg gccgcctggc atctgcggtc
     4261 agtcagtgag ccgaaatccc ggcaaggttc ggacgtcaat catgaaagcg taccgcataa
     4321 cgagtagaca ggacgcagag aaggcttacc cctgcgcaac ggaaagcccg tcccctttct
     4381 gggcagacgg attgcctctt tccagaacct ggttcgcgga taacctgggg cagcacgtgg
     4441 aaggctttca cctggaagat gacgccggtc aggtgatcgg ccatatctac tgggcgccct
     4501 cagacaaggc gctggtgccc tatcggattg aagacagggt cgccttcatc tactgcgagt
     4561 gggtgcagcg ccaacaccga gggcgaggcg gtctgcggat gctctttgat gcgtttgtcg
     4621 attttctgcg ggctgatggt tacaaaggca tcctggtcga tggcacggac tatgaagact
     4681 gcatgcacca gcgccacttc gccaggcgcg gcttccgggt aatccgcgat actggcgcag
     4741 gcaggttgat gtatctgccc ttgagccagg agagtgtcca ggtggagccc ctgtcatctc
     4801 gcatcacgcg cgaaggaacc gcgccggtcg aggtgcgcat tatcgggtcg cgcttctgcc
     4861 ccgtgggcgc gtcggcagtg ttggcgcttc ggaaggcggc cagcgagttc ggtgatcgag
     4921 tgacggtgaa agagatgccg gcaggcgccg acgcgcttga ccgatatggc gtggccgacg
     4981 ggattttcat caacggccag gtcaggttct ttgggccggt caccgaagag caggtgcgcc
     5041 aggccattcg cgaggcaatc gcgtcggtgt gagccgacga cagggggatt cctcttatgg
     5101 acaaccacac actttggaat ctggccggtg aggccgagga cgcgactgtc gctcattacg
     5161 aaccagtctt caatcgcttc agcgccgaca gcggattgga cgatcccacc atcggcttgc
     5221 tgctggcagc cgtcaccttc gtaccagaga cgacgagcgc ggcccgattg cttgtgcgcg
     5281 gcccgtacac cgccgccgag acttatctgg cccgactggc cgccgtaact gaaaagggct
     5341 acatgactga ggtatcgccg ggcgaatacc gcttgaatga cctcggtcgg gctgaggttc
     5401 aacggctcat cgaggaagca cgcacggtca tggcccaggt tgatccactg cctctggccg
     5461 actcacaacg gctggcgatc ctgctcgacc agatagtgca agccagcctg aacacaccac
     5521 ccccgcccga tacctggtct attcgcctga gtttcaagct catgccggcc accagtccgc
     5581 cgctgcccta catcgagcag gcggtctcgt gcttgagcgg ctaccgcgac gatgctcatc
     5641 tggcggcctg gcacccaggc ggcctgagcg ccgccgcgct ggaaacgctc acggtgttct
     5701 ggcgtggcga agcagactcg ctggcggcag tgtgccaacg actgagtcga cgcggtcact
     5761 cgcctgacgt ttatgcagag gcactgagag agttgcgcac acgcggcttc gtcaaagggc
     5821 cggaggtggc gccgcgcctg accgaagcgg gacgggcctt ccgtgagcag gtggaacgcg
     5881 acaccgaccg ctatttcttc gcgccgtggg cctgtctgga cgaggcgcag cgcgtcgagc
     5941 tggccgaatt gctgactcgc ctgcgtggtg ggttgcggga aagccgtgca tgacccacga
     6001 tcgacacggg tttttctttg cgattgtcgc gatgctggcg gcttgtgcac cgacgacagg
     6061 agcagatatg acgatcaggc ctgcatccac tccgtcacta cacatcagca tgactggcac
     6121 tcctctgcct caagcgattg cactgccgac tcctcaactg aagggcaggc tcacgctcga
     6181 agaggcgctg gccttgcggc ggtcggtgcg cgacttttcg gacaccccgc tcacactggc
     6241 ggagttgggg caactgctgt gggcaggaca gggcgtcact cacccggccg gcctacgcac
     6301 cgctccctcg gccggcgcgc tgtatccgct ggaagtctac gcggtgatgc gcgagggtgt
     6361 atatcattac gaaccggaga cgcatcgcct gactgtacag attcaaggcg atgtgcggca
     6421 ggcgttgcac gaggcggcgc tgcggcaaga ggcggtgttg aacgcgccgg cggtgatcgt
     6481 gatcgcggcg gtgtacgcgc gcacgacgca gaagtacggc gaggaacgca gcccgcgcta
     6541 cgtccatctg gaggcgggtc atgccgcgca gaacgtcctg ctgcaggcgg tcgcgctcga
     6601 tctcggcgca gtgccgatcg gcgcgttctg ggatgatcaa gtgaagcagg ttctggcgct
     6661 gccgcctgac cagcaaccgc tgtatttaat accggtggga catcccaggt agcggggcgc
     6721 ggctgctggc gaaacgcctg ctcaagtgag gagcaccgtg agtctcgttc ctggtacaga
     6781 catcttcctc aagcgttccg ggttgtcctc cccggcaaac agcctgctgc tgacgctcgt
     6841 gatcgccggt gttgctctcg ccgcaagaga attctttcag gtgtttgatg gactattcgt
     6901 cggctcgacg ctgatcagcc agggcattgt catgtggctg gggttggtca tcgtcgcgca
     6961 gatgattgtg cgtcgtgact ctctcaaagc tcgttgggac gaacgcgcgt tttcgatcgc
     7021 ctttcgctgg tgcgcgattc cgggcctgac gctcgtcgcg atggggatag cccatttcgc
     7081 atggattgaa ggcgcacgca tcctgccgcg cgagatcgca ctgatcccgt tcgtgtatct
     7141 gctcgtcacc ggcatcgtgc tgtggctgcg cgcgttgatg gtcttcggca tggataatct
     7201 gtcgctgatg tacgtctact tttcaagtga aagccgattg gtgaactcga atgtttatgg
     7261 cgtgctgagg catccgattt attcagccgt gatccgcatc gcgttcgcgc tcattctctg
     7321 gaatggcagc gggttcgcgt tgttcgccgg cgtcttcgtg ccgctcagca tgacggcctg
     7381 gttgcgttgg gtcgaagagc cggagctgat cgagcggttc ggtgagagct atcgcgcata
     7441 tcgggcgcgg gtgccggcgt tcttcaactt gaatccccaa acgtggccgg tgctgtggag
     7501 gttcctggtg accggcaagt gagtggtgaa gaggaagcac gagggtgtcg agtggctgga
     7561 gcagctcgtc cgggactgct agctctcacg gttcatcgtt gtgaaatcca agaagaggaa
     7621 cgctctgcgt tctcagctcg cttcgcagtc tgcggtgaga actcgctcag agtgcttgac
     7681 gcctgacgcc ctttcgtaac gtcgaaggaa ttcctctgcc gacatccata cctcgctgcg
     7741 gcgcagccgg agcagacgcc gctgtcgcag gctggctgtg ttgaaggtcc agatcagcat
     7801 ccacgtatag aagttctccc agaactcgcc gaagcgtccc agcggagtat ggtacggcaa
     7861 aaccgtgaag cctaatcgcc gcatgagact ctttccgccg gaagggtcgc tgaccgtgag
     7921 cagcaccgtg acaccaccca cagcctgcgc gttcagcaag cgcggatcgt gtcgaatatg
     7981 gacggccaca gcctgaaacg aatcgatcaa catgcgatgc acccgtcgcg cccaggcgat
     8041 gtcgggacct tcatccggaa tgcgtggaat gtgttcattc cacagatgca attccacgac
     8101 cggcgcgccc gcaccgacct caccgtcagg cagaggcagg acatgaggtg cgcgtgacat
     8161 gcgcagccgg aaaaggcagt tcggcgccgc gcagaattcg tacacgtcgt agatgcagcg
     8221 cagcagtcga tcaagtcctt gaatgagcgc acgcatggtc tgctccgacc aggtgctcat
     8281 gttacagccg tgccgccacg cgagccacca cgcaggtgtc ccgcttcaga accgaccgtg
     8341 cctggcgaca caggatgcat ccggccctcg tgcaccaaca cgaactcctc gccacgccag
     8401 atcacgcgcc ggccaaagcc gccagcaagc cacaccagcg cgctcagcat gtcacgcacc
     8461 ggcaggggcc tggctcgccg gaagaagtca tagaccaggc acagggcgac cagccaatac
     8521 acccaactgc aaatgatcag cagtacgagc aagccttgca acatctcact tcaccttatg
     8581 gctcgtgtca tctggcggat gaagaggcgc cgatgaacct tgcgttgcag cgtgcagcgc
     8641 cggataggta gtcaagcgca gaccgcgctc ctggatcacc tgccgcacgg ccgggctgag
     8701 gagcgtcgct agatcgccgg cgttggggcc gagagtctca ctcccagatc ctgtagttgg
     8761 atgaaaatag agttcggccg tcggcacact caggcggcgc agaacttgta tcacgtagtc
     8821 ttctcgcatc tagccggtcc gcatcaaacc atacacccga tgtgccacga tcgggcgatg
     8881 gtttcgcagg cggctcacac accaacggca caacagacgg aagacgatgg cccacatgat
     8941 cttcgttgcg gcccgctgcc gattatgtcg caaggccagc cagagttcgt cccgaggcag
     9001 acgaacgccc cgcgcgccaa acttcacggc cagcggcagc atcaggtcga acacaatcgg
     9061 gtgcgggtgc aggtgcaggt ggctatcgac atgcgagagc ggcagccccg tggtcgtgaa
     9121 ccgttcgaac tgggcagcca attctcgcgt cagctccgca cgagccatgc ggctaaagta
     9181 gtagcgcaag ccgatgcgaa tcgggtcgtt ggaaaaatgg ccgtcgccat cgaccaggtg
     9241 gggtatctcg ccgggtgaca agacagagcg cccatttgcc accacgacgt gcagtccgac
     9301 ggccagcgtc ggcgtttccc gagccaacgt tatcgcctcc tcgacggcgt ccccggtcac
     9361 catcaggctg gcgctggtga gcacgccctc gtagtgggca cgtatcacgg ctgtgttgat
     9421 gccggtcgag cgaccaaagt catcggcgtt tagaatgatc cacatctagt tgagtttcca
     9481 actcggccac acgaacctcg cgcggatcgg gcgtcagttt caaagcgttt ttccgggttg
     9541 atcatctcat cctaatgtag atcactcgcc gccgcttgtc tattgtcaaa tggatgctct
     9601 tttccggctc tttggcacag gtggacggtg tcgagccaag ttgaacgcta aaccagtacg
     9661 tattttgaat gccgctaacc tcagagtgcc gcttgaatca ggggccgggc ggtggggcgc
     9721 gcgtcgccgg ggcagtagtg agctgggcct gccgccccgt tgcgggagcg gatcacgact
     9781 gtcgtatcca aggtggggcg acatcctgta tcagttcttg accgagacgc tcacgctgtc
     9841 agtgctgggc ggcgtgatcg gcatcctggc tagcgcgggc gtctcggcgc tggtgaatgc
     9901 gtcggggttg atcgatacgg tggtgtcgat ggaatccatc gtgctggcct tcggtttttc
     9961 ggcggcggtg ggtgtgttct tcggcatgtg cccggccaat tacggatagg ctccaagttg
    10021 atccagtgcg aagcagtgac ttgcgctgcg cgcg
//
LOCUS       DBNHXB010000140         4299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_365544, whole genome shotgun sequence.
ACCESSION   DBNHXB010000140 DBNHXB010000000
VERSION     DBNHXB010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_365544"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /gene="hemW"
                     /locus_tag="AC162G_05490"
     CDS             complement(<1..723)
                     /gene="hemW"
                     /locus_tag="AC162G_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR5075298.1"
                     /translation="MHDCVGVYIHIPFCHHRCTYCDFNIYAGMRSLRERYVTAVAKDV
                     AAAPADLPVPTIYFGGGTPSLLPAEQIARILDAIRTQFDVAPDAEITLEANPRTADDG
                     YFDALRALGVNRISLGMQSALERDLHLFRRGHTFGDVVQTVRLARSAGFENLNLDLIY
                     GIPGQSPADWRHTLEAALWLEPNHVSAYCLQVEDGTTLKKWIVQGKVLPPDDEVAAEL
                     YNLAEEILEEAGFVHYEISNWAR"
     gene            989..1690
                     /locus_tag="AC162G_05495"
     CDS             989..1690
                     /locus_tag="AC162G_05495"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003967394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5075299.1"
                     /translation="MGEISVNALDRVDFTVEKGEFVAIMGPSGSGKSTLLHLLGGLDG
                     ASGGEVTLAGKPLSRLSDNEVTLVRRRNVGFIFQFYNLVPTLTAEENIALPLMIDGKK
                     MEDCRERIDDLLNLVGLADRRHHKPDQMSGGQQQRVAIARALVTDPDILLADEPTGNL
                     DSKSGTAILELLRRTCDELGHTIVMVTHDARAASYADRVVFLKDGRVVDQVRRADADP
                     HHTMEAIIDVMRKLD"
     gene            1804..>4299
                     /locus_tag="AC162G_05500"
     CDS             1804..>4299
                     /locus_tag="AC162G_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR5075300.1"
                     /translation="MRPRKTLRSLNRLALRNLAARKTRTILTTLGIVVGVAVILAISI
                     TNESTLSSINTIFDEASGLAHLVIAPSSYTGVNGFPESILARVRSEPSVAYAVPTLQA
                     RTLLARDAEDWQFEFGLGGTSGGNDLAIVGIDPELDTKARLYKLETGRFLEPGETSYS
                     ALFTQEFAEEKNITLGQDVDILLPAQSGGTATLRAVGFLRKDGPGLINSGSVAFTPLE
                     ALQEIFARSGELDQIDVVAQPEVAASSERLADLKSRLEEKLGTKYVVSYPAQRGQIVT
                     QQLATYQQGLSFFSMIALFVGGFLIYNTFTMTIVERTQEIGLLRALGLSRVQVTRLIL
                     IEALVLGAIGAALGAGFGLALARGLIASMSTVTAMEILSVSVPLDGLLISVTVGLGVT
                     IVSALVPALRAGRVSPLAALRVKGQENPSGAPGKLALFAGIELVAVALLVINTVPLRP
                     SLSFPVGSGMVFVMFLGATLTVPSVVQNLEALFRPAIVRIFGAEGRLGAGNIERSRGR
                     ASLTVAALMTGIAMVISIGAMSLSFQTDIDSWVNSAVGGDLIVRSPVPMRIDFASRLE
                     TIPGVLAVTPARYFGVRPAQDETGLTFQAIDPNTYFQVASFQYAEDKEDAAQIERELA
                     SGDLLVSTTVADRYDLERGDTFELETRRGVHAFRVAGLVSDFTSQGYVINGTLDTMRR
                     YFGIDDVDRFTLKLAPGADLAAVKAAIEDRYGRSRNIQVETTEEFRNRVRELTNRAFG
                     LLDVLANIGVIVAALGVINTMMMNVLERRREIGALRSIGMTRAQVIRMILAEACAMGA
                     AGAIFGIAFGVLLARVMVDGMQQMSGYRLTFEMP"
BASE COUNT          787 a         1351 c         1376 g          785 t
ORIGIN      
        1 acgcgcccaa ttcgatattt cgtaatgaac gaacccggcc tcttcgagta tctcttccgc
       61 gaggttgtac agttctgcgg caacctcgtc gtcgggggga agtactttgc cctggacgat
      121 ccatttcttc aaggtcgtgc cgtcttccac ttgcagacag tatgccgaga catgattggg
      181 ttcgagccac agcgcggctt ccaatgtatg gcgccagtca gccggcgact ggccgggaat
      241 gccgtaaatc agatcgagat tgaggttctc aaatcccgcc gagcgcgcca ggcgaaccgt
      301 ctgcacgacg tcgccgaacg tatggccgcg ccggaataaa tgcagatcgc gctcgagcgc
      361 cgactgcatg ccgaggctga tgcgattcac gccgagcgcg cgcagcgcat cgaagtaccc
      421 gtcgtcggcc gtgcgcgggt tcgcttccag cgtgatctcg gcgtcgggcg cgacgtcgaa
      481 ctgcgtccgg atcgcatcga gaatccgggc gatctgttct gccggaagga gcgaaggcgt
      541 gccgccgcca aaatagatcg tgggaacagg caggtcggcc ggcgccgcag cgacgtcctt
      601 cgccacagcg gtcacgtatc gctcgcgcag cgatcgcatg ccggcgtaga tattgaagtc
      661 gcaatacgtg cagcgatggt ggcagaatgg gatgtggatg tagacgccga cacagtcgtg
      721 catagctcct atggtactcc tctgcgaaaa ctacgccaag cgcagtagac ctgggcgatt
      781 gcgagttcat tttgcacctt gaccgtgtcg gaatacttcc cagtccgtgt gccaggtcta
      841 tgtttggtga attcttcaca aaccggatat aattcaaagc gtcaacgttc caacgttgga
      901 cgcgcccacg ttggaacgta tcttgtcggc tgctggaggg tgaagcgtgg cggtcttaca
      961 gacggacaaa gtgaccaagc agtaccggat gggcgagatc agcgtcaatg cgctcgaccg
     1021 ggtcgatttc accgtcgaga aaggtgagtt tgtggcgatc atggggccat ccggttccgg
     1081 caaatcgacg ctcctgcacc tgctgggcgg tctcgacggc gcgagtggcg gcgaagtgac
     1141 gctcgccggg aaaccgctgt ccaggttgag cgacaacgag gtgacgctcg tgcggcgtcg
     1201 caacgttggc tttatctttc agttctataa cctggtcccg accttgaccg ccgaagagaa
     1261 catcgccctt ccgctgatga tcgacggcaa gaagatggaa gactgccggg agcgcatcga
     1321 cgatctcctg aacctggtcg ggctggccga tcgcaggcat cacaagcccg accagatgtc
     1381 gggcggccag cagcagcgcg tggcgatcgc ccgcgcgctc gttaccgatc cggacatcct
     1441 cctggccgac gagccgacgg gcaacctcga ctcgaagtcc ggcacggcga tcctcgaact
     1501 gctgagacgc acgtgcgacg agcttgggca caccatcgtg atggtcacac acgacgcgcg
     1561 cgcggcttcc tacgctgacc gcgtcgtctt cctcaaagat ggccgcgtcg tcgaccaggt
     1621 gcgccgcgcc gacgccgacc cgcatcacac catggaagcg atcatcgacg tgatgaggaa
     1681 gctggactga tcagtaatca gtaatcggta atcagtaatc agtgagcgcc gattactgct
     1741 caccatttac tgaacactga taactgatca ctgatgactg atcactgatg actgatcact
     1801 gaaatgcgac ctcgtaaaac acttcgcagt ttgaaccggc ttgctcttcg taatctcgcc
     1861 gcgcgcaaga cgcgcacgat cctgacgacg ctcggcatcg tcgtcggcgt ggccgtcatc
     1921 ctcgccatca gcattaccaa cgagagcacg ctgtcgtcta tcaacaccat tttcgacgag
     1981 gcgtccgggc tggcgcacct ggtgatcgcg ccgtcgtcgt acacaggcgt caacggtttc
     2041 cccgagtcga tcctggcgcg cgtgcggagc gagccgagcg tcgcatacgc tgtgccaacc
     2101 ctacaggcgc gcacgctgct ggcgcgcgac gccgaagact ggcaattcga attcggcttg
     2161 ggcggcacga gcggcggcaa cgacctcgcg atcgtgggca ttgacccgga actggacacg
     2221 aaggccaggc tttacaagct cgaaacgggt cgctttctcg aaccaggcga gacgtcttac
     2281 tcggcgctgt tcacccagga gttcgccgaa gaaaaaaaca tcacgctcgg gcaagacgta
     2341 gacatcctgc tgcctgcgca gtccggcggg acggcgacgc tgcgcgccgt cggtttcctg
     2401 cgcaaggacg ggccgggcct gatcaacagc ggcagcgtcg ccttcacgcc gctcgaagcg
     2461 ctccaggaga tcttcgcccg cagcggcgag ctggatcaga tcgacgtcgt cgcgcagccc
     2521 gaggtagccg cctcgagcga gcggctggcc gatttgaagt cacggcttga agagaagctc
     2581 gggacgaaat atgtcgtgag ctatccggcg cagcgtgggc agatcgtcac gcagcagctc
     2641 gccacgtacc agcagggtct gtcgttcttc agcatgatcg cgctgttcgt cggcggcttc
     2701 ctcatctaca acacattcac gatgacaatc gtcgagcgca cacaggagat cggcttgctg
     2761 cgcgctctgg gcctgtcgcg cgtgcaggtg acgcgcctga ttctgatcga ggcgctcgtg
     2821 ctcggcgcga tcggcgccgc gctcggggcc gggttcggcc tggcgctggc gcgcggcctg
     2881 atcgcctcga tgagcacagt caccgccatg gaaattctca gtgtgagtgt gcctctcgac
     2941 gggctgctga tcagtgtgac ggtgggcctg ggggtaacta tcgtttcggc gctggtcccc
     3001 gcgctgcgag cgggccgcgt cagcccgctc gccgcgctgc gcgtcaaagg acaggagaac
     3061 ccgtctggcg cgccgggcaa gctggcgctg ttcgcgggca tcgagttggt cgccgtcgcg
     3121 ctcctcgtca tcaacaccgt gcccttgagg ccgtcgctct ccttccccgt cggctcgggg
     3181 atggtcttcg tgatgtttct cggcgcgacg ctcaccgtgc cgtcggtcgt ccagaacctc
     3241 gaagcgctgt tccggcccgc catcgtgcgc attttcggcg cggaaggccg cctgggcgcg
     3301 ggcaatatcg agcgctcgcg cgggcgggca tcgctgaccg tcgcggcgct catgaccggc
     3361 atcgccatgg tgatcagcat cggcgcgatg agcctctcgt tccagactga catcgacagt
     3421 tgggtgaact cggcggtggg cggcgacctg atcgtgcgct cgccggtgcc catgcgcatc
     3481 gacttcgcgt cgcggctcga gacgatcccc ggcgtgctgg ccgtcacgcc cgcgcgctac
     3541 ttcggtgtgc ggcccgccca ggacgagacg ggcttgacct tccaggcgat cgaccccaac
     3601 acgtatttcc aggtcgcctc gttccaatac gcagaagaca aggaagacgc cgcgcagatc
     3661 gagcgcgagc tggcgagtgg cgacctcctg gtgtccacaa ccgtcgccga ccgatacgac
     3721 ctcgagcgcg gcgacacgtt cgagctggag acccggcgcg gcgtgcacgc cttccgcgtc
     3781 gccggtctcg tcagcgactt caccagccag ggctacgtga tcaacggcac actcgacacg
     3841 atgcgacgct acttcggcat cgacgacgtc gatcgcttca cgctcaagct cgcgccgggc
     3901 gccgatctgg cggcggtcaa agcggcgatc gaggacaggt acgggcgaag tcgaaacatc
     3961 caggtcgaga ccaccgagga gttccgcaac cgggtgcgcg agctgacgaa ccgcgccttc
     4021 ggcctgctcg acgtgctggc gaacattggc gtgatcgtcg ccgcgctcgg cgtgatcaac
     4081 acgatgatga tgaacgtgtt agagcggcgg cgcgagatcg gcgcgctgcg cagcatcggc
     4141 atgacgcgcg cgcaggtgat ccgcatgatc ctcgccgagg cgtgcgccat gggtgcggcg
     4201 ggcgcgatct tcggcatcgc cttcggcgtg ctgctggcgc gcgtgatggt ggacggcatg
     4261 cagcagatga gcggctatcg cctgacgttc gaaatgccg
//
LOCUS       DBNHXB010000141         7579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3049675, whole genome shotgun sequence.
ACCESSION   DBNHXB010000141 DBNHXB010000000
VERSION     DBNHXB010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3049675"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(484..2586)
                     /locus_tag="AC162G_05505"
     CDS             complement(484..2586)
                     /locus_tag="AC162G_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR5075301.1"
                     /translation="MKARPSFTRRQFIKIGAAAGAGLLVPWRFSVGRAYAQTPVPGGS
                     VLKYQTPLLIPPVMPKAGTLTDPMGKPIDYYEISMKQFRQVILPASFYANQTGDARFG
                     TLVWGYGAVASASKRGLLLHNAPSLTIEAMWRRPVRVKWINDLKDPVTGNFLPHLLPV
                     DPTLHWANPPQGSDPAYGSGPRDSRPDFSSTPGPYTGPVPIVTHVHGAVGVGDESDGY
                     AEAWYLPAANGIPEGYAREGTWYNFFAGKAATKFGATWGPGFATFQYPNHNRASTIWY
                     HDHALGMTRLNVYAGPAGFYLIRGGPAGDAAVIDSRFGTTAVLPGPAPKASDKFPPNK
                     TYYEIPIAIQDRSFNDDGSLFYPAGRDFFDEFTGPFIPDSNVSPIWNPEFFGNMIMVN
                     GNTWPFQVVEQRRYRFRFLNGCQSRFLILDFSNIPGVEVWQIGNEGGFLAAPVNITAD
                     HGNRLLMALAERADMIVDFTNVPVGNYVLGNVGPDEPFGGGVPDVDFDPSDPDSTGQV
                     MEFRVVPAVAPDPTTPPQFLVLPAIAPLPAETFTRPLALIEMMSNVHDGPSEAVLGNV
                     DGGMAMHKHWADPVSEIPNVGDTEVWEFYNFTADAHPMHIHEITFEVVNRQAITFTED
                     MGHISDVTPVGVPRGPEAWESGPKDTVISYPGEVTRLRAKFDTPGQFVWHCHIVEHED
                     NEMMRPYRIGPVQQGQPE"
     gene            complement(2841..3692)
                     /locus_tag="AC162G_05510"
     CDS             complement(2841..3692)
                     /locus_tag="AC162G_05510"
                     /inference="COORDINATES: protein motif:HMM:NF017416.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5075302.1"
                     /translation="MPVPSGMFPPLPADTARAAADVFNIHNIYLSIGDRIDELLADMR
                     PADLDVSGEQPPGSLAMLALVTCFQYAENIPDRHAADAVRTRMDWKYALHLGLSYPGF
                     DPLVLCEFRRRLLQNPPAQRALDHILAWLGQIGLFASRDRRQATAIQVIDTVCRLTRL
                     EQMVEAIHQTLEALAAAQPEWLRAIAPAQWLQRYTSTSATMSLPGSREVQERMAQAIG
                     VDMLYLLDAITQAHKPELNALPEVQWLRRVWPRHFEKRLEQVCWRGSCWNQCCLSDHT
                     NSNHKIC"
     gene            complement(3921..4568)
                     /locus_tag="AC162G_05515"
     CDS             complement(3921..4568)
                     /locus_tag="AC162G_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5075303.1"
                     /translation="MTVRILIADDHGVLRAGLRALLNAEPDLQVVGEAADCDEVVRLS
                     GVLRPDVVLMDISMPGPGGGIEATRRMVELLPDTCVLILTAHEDKGLLREAIRAGAAG
                     YILKRAAESELVSAIRAVARGDLYVHSALTRALLADAVQPSTGGAGGAESLSPREIEV
                     LRLVARGYTNRQIAEALNLSVRTVESHRANLMDKLGLHSRVDLVRYASDHNLLAS"
     gene            complement(4565..7420)
                     /locus_tag="AC162G_05520"
     CDS             complement(4565..7420)
                     /locus_tag="AC162G_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR5075304.1"
                     /translation="MTIRSRLTIAFVLIVLLSAAAISVFSSLSYLQTERRQAVEHLDA
                     LAAMVENRFEEWVSHLQATLAVAMARPMDAVFAHALTSESREAHAHLQDDFEHIAGSG
                     RVFAEVFLMDNRGNVVVSTDTGRRGAFDGGSAYFHAGLSGPYVGTPFYDAERRRMVVI
                     AARPVIDRQGTLLGVLAGRANLASFADPAFSQAGLGETGVMYLVDADRALLTDFRFSD
                     AGANEVTGAYQARVTYVVDTEGANAVTNSRGHGFGLYEGVRGAPVVGVYRWLPSLQAA
                     LLIELEQAEAFRSAYDVLLVDAGVAAAVLLVALGVALSVVRSITSPLATLAETAARIA
                     GGDLDVEVRVERQDEIGALARAFNHMTARLRNGINSLKRHAADREQAAEMLREVHDEL
                     EMRVQQRTSELVQANAALRAEMEKGRLQATALEAAANGIVITDRMANIEWCNTAFLQM
                     TGYSFEEIAGRNLRMLTSGRHDSTLYAQLWDTVLARQAWHGRTVNRRRDGSLYTVEQT
                     IAPVFDEKGELSHFIAIKQDVTDRIRVEEEIKRRNRELAVINAASLALTRTLDLDTVL
                     DTLLEYVGRLVPYTSARVFLLEANSRLTVRAARGGEPRTHAERAGLLALDAASNRIVQ
                     ELLTGGRSLSIPNVDDVPDWDDRVSGGKALHWLGVPLATGDKVIGLCSLSSHAPDAFS
                     REHLQFAEALVGQATVAIQNAWLFAQVRAGRERLQALSRRLVEVQESERRYIARELHD
                     EAGQALTALLVSLRLLEREAHHPEAVVAGVAELKRTVDEVLEDLHRLAIDLRPASLDH
                     LGLAPALRQHAEAISDRHGLTVQFEAIGVGDCRLPPDMETAVYRIVQEALTNAVRHAR
                     ATRVDVLLEQRGGRLITVVEDNGIGFDPDAAQRGGRLGLFGMRERAEMFGGALVVESE
                     AGRGTTVLLEMSCDATHPHRPTAPTAPSVSQRSGDAL"
BASE COUNT         1423 a         2398 c         2378 g         1380 t
ORIGIN      
        1 tccgccacat tccgctggat ttgggaatcc agcgggagca aattgtgatc taccgagtct
       61 gggtgacacg cacgctctgc gccattcagg aagtccgctt tagaaggtct ctatagggac
      121 aaacgcgcaa tacagataag tgagcatggt gaacaacaag cagaccaatt cattataccg
      181 gagccggatg caacaggata tggctatttt gtatgaaatg gtatgatttg cggttagaat
      241 gcttattctt gcagagcctg gtatgaccaa ttgcccatca cgagatcacg attcaacacc
      301 tcacaccgaa atcaaaaaat ccttaccaga gacacgcgag agggccgcac cgggcggccc
      361 tctctttgac tatgtacgct ccactctacg gcacgaacgg cggcaagcct ccgaccgtgt
      421 gcggacacat catctggtta ttctcgcatc cgatggacgc gccaggcggt ccatagactc
      481 ctgttactct ggctgacctt gctgcacagg cccaatgcgg tacggccgca tcatctcgtt
      541 gtcctcgtgc tcgacgatgt ggcagtgcca tacaaactgg cccggcgtgt cgaacttggc
      601 ccgcaagcgg gtgacctcac ctggatagga aataactgta tccttagggc cactctccca
      661 ggcttcagga cctcgcggaa ctccaaccgg agtgacatca gaaatgtggc ccatatcctc
      721 tgtgaaggtg atggcctgcc ggttcaccac ttcaaacgtt atctcgtgga tgtgcatcgg
      781 atgggcatcg gctgtgaagt tgtagaactc ccaaacttcg gtgtcaccca cgttcgggat
      841 ttcgctgacc ggatcggccc agtgcttgtg catcgccatg ccgccatcca cattgcccag
      901 cacggcctcc gatggcccgt cgtgaacatt ggacatcatc tcgatcagtg ccagaggccg
      961 cgtgaatgtt tcagccggaa gcggcgcgat ggccggcagc acaaggaact gtggtggcgt
     1021 ggtgggatcg ggcgcaaccg ccggtacgac acggaactcc atcacctgac cggtcgaatc
     1081 cgggtctgaa ggatcgaagt ccacatccgg gacgccgccg ccgaacggct cgtccgggcc
     1141 gacgttgcca aggacgtaat tgccaaccgg cacgttggtg aagtcgacga tcatgtcggc
     1201 gcgttcggcc agggccatga gcagccggtt gccgtggtcg gcggtgatgt tcaccggcgc
     1261 ggccaggaag ccaccctcgt tgccgatctg ccacacttcc acgccgggga tgttgctgaa
     1321 gtccaggatc aggaagcgcg actggcagcc gttgagaaag cggaagcggt agcggcgctg
     1381 ctcaaccacc tggaatggcc aggtgttgcc gttcaccatg atcatgttgc cgaagaactc
     1441 ggggttccag atcggcgaga cgttgctgtc cgggatgaac ggtccggtga attcgtcgaa
     1501 gaaatcgcgc ccggccggat agaacagcga gccgtcgtcg ttgaaggagc ggtcctggat
     1561 ggcgatgggg atctcgtagt aggtcttgtt cggcgggaac ttgtcactcg ccttgggggc
     1621 gggacccggc agcacggccg tggtgccgaa tcggctgtcg atcacggcgg cgtctccagc
     1681 cggcccgccg cggatgaggt agaagccggc cgggccggcg tacacgttga gccgggtcat
     1741 gcccagcgcg tggtcgtggt accagatggt cgaggcccga ttatgattcg gatactggaa
     1801 ggttgcaaat ccgggccccc aggtggcgcc gaatttcgtc gcagccttgc cggcgaagaa
     1861 gttgtaccag gtgccctccc tggcgtagcc ttcagggata ccgttcgccg cgggcaggta
     1921 ccaggcctcg gcatagccgt cgctctcgtc gccgacgcca accgcgccat gcacgtgggt
     1981 gacgatcggg acggggccgg tgtacgggcc gggcgttgag gaaaagtctg gccgcgagtc
     2041 acgcgggcca ctaccatacg ccgggtcact gccttgcggc gggttggccc agtgcagtgt
     2101 cggatcgacc ggcagcaggt gcggtaggaa gttaccggta actggatcct tcaggtcgtt
     2161 aatccacttg acgcggaccg gcctcctcca catggcctcg atggtgagcg agggcgcgtt
     2221 gtgcagcagc agccctctct tgcttgccga cgcaacggcg ccgtagcccc agacgagggt
     2281 gccaaaacga gcatcacctg tctggtttgc gtaaaacgat gctggcagaa tcacttgccg
     2341 gaactgcttc atcgaaatct cgtaatagtc gatgggcttg cccatcggat ccgtgagcgt
     2401 tcccgccttg ggcatgacgg gcgggattaa caatggggtc tggtatttga gcaccgagcc
     2461 gccaggaaca ggggtttgcg cgtacgcccg cccaacgctg aacctccacg gcacgagcag
     2521 gcccgccccg gctgccgcgc cgatcttgat gaactgacgc ctggtgaaac tgggtctagc
     2581 tttcatgttc tccctccatg tcgatcgtat gtaaaagcct cccgacccgt agctgactta
     2641 cgtcgcatct gcagcgacaa tagtatgaac acgaacctga gagtcttgct tgtaagcacc
     2701 tccttccaat gactgccgat gatccacaag tgccccaggc cctcatcctc ataggttgtc
     2761 tgcgggacgc ggacggtaag caaatgggca caaaagatat accacagcga gtgcggtgcg
     2821 acacagctat ttcgtttgaa ttagcatatt ttgtggttag aatttgtgtg atcagacagg
     2881 cagcactgat tccagcacga accacgccag cagacctgct caaggcgttt ttcaaagtgc
     2941 cggggccaca cccgccgcag ccactgcact tccggcagcg cgttcagttc cggtttgtgc
     3001 gcctgagtga tggcgtccag caaatatagc atgtcgaccc cgatagcctg cgccatcctt
     3061 tcctgtactt ctctgctgcc cggcaggctc atcgtcgcgc tcgtcgaagt gtatcgttgg
     3121 agccactggg ccggcgcgat cgcgcgcagc cactcgggtt gggcggcggc cagcgcttcg
     3181 agcgtctgat ggatcgcctc taccatttgc tccaagcggg tcagcctgca caccgtgtcg
     3241 atgacttgaa tagccgtcgc ctgccgccga tcgcgacttg cgaaaagacc gatctgaccg
     3301 agccaggcca ggatgtggtc caatgcccgc tgcgcaggag gattctgcaa gagccgccgg
     3361 cggaactcgc acagcaccaa agggtcaaag cccggatagc tcaatcccaa atgcagcgcg
     3421 tatttccagt ccatgcgggt gcggacggca tcggccgcgt ggcggtccgg aatgttctcg
     3481 gcatactgga agcaggtgac cagcgccagc atggccagcg agccgggcgg ctgttcccct
     3541 gagacgtcca ggtcggccgg ccgcatgtcg gcaagcagtt cgtcaatccg gtcgccgatg
     3601 ctcaggtaga tgttatggat attgaacaca tcggctgccg ctcgggcagt gtccgccggg
     3661 agcggcggaa acatcccaga aggcaccggc atgttggcct cgatctgcaa gtgtcaggat
     3721 tgctccgatg atacgcgccc aggcgtgcgg tcgtgaagcg tagaaaaccg ccagttttgc
     3781 gctggcggtt gaggaaaact acggaacgga taggcgatcg gctccgtaga aactacggtc
     3841 ttcaaatccc aaacttcaaa cttcggattt tggattttgg attttgaaat ttggagcttg
     3901 cagtttggat ttccgaagta tcaggatgcg agcagattgt gatccgaagc gtagcgcacc
     3961 agatcgacgc ggctgtgcag gcccagcttg tccatcaggt tcgcgcggtg gctctcgacc
     4021 gtccgcacgc tcaaattcag cgcctccgca atctgccggt tggtataccc gcgtgcgacg
     4081 aggcgcagca cctcgatctc gcgcggactg agcgattccg cgccgcctgc tccacccgtc
     4141 gagggctgca cggcgtcggc caacaaggcg cgggtcaaag cggagtggac gtacaagtcg
     4201 ccgcgcgcga cggcgcggat ggcgctgacc agctcggact cggcggcgcg cttcaggatg
     4261 tatcccgccg cgccggcgcg gatcgcctcg cgcagcaggc ctttgtcctc gtgagcggtg
     4321 aggatcagga cgcaggtatc gggcagaagt tccaccatgc gccgggttgc ctcgatgccg
     4381 ccgccgggac cgggcatgct gatgtccatc agcacgacgt cggggcgcag cacgccggac
     4441 aggcgcacga cctcgtcgca atcggctgcc tcgccgacga cctgcagatc aggctcggcg
     4501 ttcaggagcg cgcgcaagcc cgcacgcagg acgccgtgat catccgcgat gagaatccga
     4561 accgtcacaa agcatctcca gatcgctgag acactgaggg cgctgtgggc gcggtcgggc
     4621 gatgaggatg cgtggcgtca caagacatct ccaaaagcac cgtcgtgccg cgcccggctt
     4681 cgctctcgac gacgagcgcg ccgccgaaca tctcggctcg ctctcgcatg ccgaacaggc
     4741 ccagtcgccc gccgcgctgc gccgcatcgg gatcgaagcc gattccgttg tcttcgacga
     4801 ctgtgatcag gcggccgccg cgctgctcca gcagcacgtc gacccgcgtc gcccgggcgt
     4861 gccgcaccgc gttggtcagc gcttcctgca cgatgcggta gactgccgtt tccatatccg
     4921 gcggcaggcg gcaatcgccg acgccgatcg cttcgaactg gacggtcaat ccatgccggt
     4981 cgctgatcgc ctcggcgtgc tggcgcagcg caggcgccaa tccgagatga tccaggctgg
     5041 ccgggcgcag atcgatggcg agacggtgaa gatcctccag cacctcgtcg acggtgcgct
     5101 tgagctcggc cacgccggcg acgacggctt ccggatgatg cgcctcgcgc tcgagcaggc
     5161 gcaggctgac gagcagcgcc gtcagcgcct gccccgcctc gtcgtgtagc tcgcgcgcga
     5221 tgtagcgccg ctcgctctct tggacctcga ccagccggcg tgacagggcc tgcaggcgct
     5281 cgcgcccggc gcgcacctgc gcgaacagcc aggcgttctg aatggcgacc gtcgcctggc
     5341 cgacgagcgc ttccgcgaat tgaaggtgct cgcgcgaaaa ggcgtccggc gcatggctgg
     5401 acaggctgca cagcccgatg accttgtcgc cggtcgccag cggcacaccc agccagtgga
     5461 gcgccttccc cccgctgaca cgatcgtccc agtctgggac gtcgtcgacg ttcggaatcg
     5521 agaggcttct gccgccggtc agcagttcct gaacgatccg gttcgacgcc gcgtcgagag
     5581 ccagcaagcc cgctcgctcg gcatgcgtgc gcggctcgcc gccgcgcgca gcccgaaccg
     5641 tcaagcgcga attggcttcc agcaagaata ctctggcaga agtgtaaggg acaagccgtc
     5701 ccacgtattc gagcagcgtg tcgagcacgg tgtccagatc cagcgttcgc gtgagggcca
     5761 aactggccgc gttgatgacg gccagctccc ggttgcgccg cttgatttct tcttccacgc
     5821 gaatgcggtc ggtgacgtcc tgcttgatcg cgatgaaatg ggaaagctcc cctttctcgt
     5881 cgaataccgg agcgatcgtc tgttccactg tataaagact cccgtcccgg cggcggttga
     5941 ccgtccggcc atgccaggcc tgccgggcga gaaccgtatc ccacagttgc gcgtagagcg
     6001 tggaatcatg ccggcccgat gtcagcatgc gcagattccg accggcgatc tcctcgaaag
     6061 agtaaccggt catttgcaga aatgcggtgt tgcaccattc gatgttggcc atgcgatcgg
     6121 taatgacgat gccgttggcg gccgcttcga gagccgtcgc ctgaagccgg cccttttcca
     6181 tctctgcgcg cagcgccgcg ttggcctgca ccagttcaga cgttcgctgc tgaactcgca
     6241 tttccagctc gtcgtgcact tcgcggagca tctctgcggc ctgctcacgg tcggcggcgt
     6301 gccgcttcag gctattgatt ccattgcgca gccgggcggt catgtggttg aacgcccgcg
     6361 ccagcgcgcc gatctcgtct tgccgctcga cgcgcacctc gacgtcgagg tcgccgcccg
     6421 cgatgcgcgc cgcggtctcg gcgagtgtgg ccagcggaga ggtgatgctg cgcacgacgg
     6481 agagcgcaac gcctaatgcc accagcagca cggcggcggc cacgccggcg tcgaccagca
     6541 gcacatcgta ggcagaccga aaggcttcgg cctgctctaa ctcgatcagc agcgcggctt
     6601 ggagtgacgg cagccagcgg tacacgccca cgaccggtgc gccgcgaaca ccctcgtaca
     6661 ggccaaagcc atggccgcgg gagttcgtca cggcgttcgc gccctcggtg tcgacaacat
     6721 aagtgactcg cgcctgatat gcgccggtca cctcatttgc gccggcatcg gagaagcgaa
     6781 aatcggtcag cagcgcgcgg tcggcgtcga ccaggtacat caccccagtc tcacccaggc
     6841 ctgcctggct gaacgccggg tcggcgaagg acgcgagatt ggcgcgcccg gccaggacgc
     6901 ccaggagcgt cccctggcga tcgatgaccg ggcgcgccgc gatcacaacc atgcgtctcc
     6961 gttccgcatc gtaaaacggc gtgccgacgt acgggccgga aagcccggcg tggaagtatg
     7021 cactgccgcc gtcgaacgcg cctctacgac cggtgtcggt cgagacgacc acgttccccc
     7081 ggttgtccat caagaacact tcggcgaaga cccggccact gccggcgatg tgctcgaagt
     7141 cgtcctggag gtgagcgtgc gcctctcggg attccgaggt cagcgcgtgg gcgaacacgg
     7201 cgtccatcgg cctggccatc gccacggcga gtgtggcctg caggtggctg acccactcct
     7261 cgaaccggtt ctctaccatc gcggccagcg catccagatg ctcgactgcc tgccgccgct
     7321 ctgtctggag ataggacagg ctcgagaaca cgctgatggc cgccgccgac agcagcacga
     7381 tcagcacgaa cgcgatcgtc agccggctgc ggatggtcaa ggagcggctg cgcctttcaa
     7441 actccaaatc tcaaactcca aatctcaaac tcagaagaga gattcctcac tgcgttcgga
     7501 atgacacggc gaggcgatca gcgcgggacg tgtcaggccg agcaggcacg acgaggcatc
     7561 tctaccccgg cgcgccgcg
//
LOCUS       DBNHXB010000142         5598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1205027, whole genome shotgun sequence.
ACCESSION   DBNHXB010000142 DBNHXB010000000
VERSION     DBNHXB010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1205027"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="AC162G_05525"
     CDS             <1..403
                     /locus_tag="AC162G_05525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR5075305.1"
                     /translation="SLRRVSPNTINSITVSTTTLDAYFAPLNIGPHVIKLDIEGAELL
                     ALRGAEGVLRTFRPILVVEASKELMHAFGYGCGDLIDFLRAVDYHLYTLNYSALKPIP
                     TEYRSGYENLICLPRDSSSYTALTSSGIRPI"
     gene            408..1601
                     /locus_tag="AC162G_05530"
     CDS             408..1601
                     /locus_tag="AC162G_05530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5075306.1"
                     /translation="MSTARIAFVDHAPFVGGAETVLLDLIGHLDRSRFDPIVFLPARS
                     PLVRHLDARGVPWRNLPMPRINVRSPTALLRWRRASSELARLARDCGVDLVHSNTVRA
                     HLASAFVRDVPVVWMLHDDTFPHWLFRRLRRRPARLIANSYTTASTYNLEGDPRACVV
                     HNGVDTQAEPGNRYRFRRRIGIGSDKVLVAHVGRLVRWKGQHLFIRAAARVAGEHPQA
                     HFALVGTYTEGDDSPGVPSALLRAGLSGGAAYHAELQRLAQSAGLGSRLTFAGHQDNI
                     ADVYAGIDLLVHASTRPEPFGRVLIEAMAAGLPVIASAQGGPTEIVEDGITGLLVTPG
                     SSQALAEALTKLLADASRRAEMGVAARRLAIERFDIRTQVARIESIYLDILANRARVA
                     DAKRG"
     gene            1629..2882
                     /locus_tag="AC162G_05535"
     CDS             1629..2882
                     /locus_tag="AC162G_05535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HIR5075307.1"
                     /translation="MRSSPTVSLVMAPLLLAGYLIWGGWAITHDKPIDFYAYYAAADA
                     LNRGQPLYHANPEQEALNPIPGLPGILPPYIYPPALASLLRPLARLTPPVAAFLWMIL
                     NSVGLVLGAWILGTSLDDERGRQVALLAMAGFVPPLTSLYAGQFNGLVWLGLAAIFHL
                     GIGPRQFGMNGQPCPEQSRRDAGRRSIAAALSGIILGATILLKPLLWSSAIFFAWQRR
                     WLVVLTCIAGTLLLVLVSAAFDGLTPWREFLSIAPQLSSTTTTAYPPNQSLYGFALRA
                     FTTHEYGASLALQPAWAAPFAALLSLALVGATMALTWNRSGAQQPARFALWLITGLLV
                     TPLSWLHHLLLALLPILIIGTVALKHRAWGALAACGLAYLLIDVQGLAWHSLVGNTLL
                     LSLGTYGMLILWSILAVWLVRNKLE"
     gene            2894..4222
                     /locus_tag="AC162G_05540"
     CDS             2894..4222
                     /locus_tag="AC162G_05540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR5075308.1"
                     /translation="MRAFIRERRLLIVVFIVLFPLLFRPRVYGFDPVGYFSWLRSAVI
                     DGNLDTTDEYLRYGNEKVVGPTVTGYNHNPYAIGAPLLWSPFYLAAHAVESGRSAITQ
                     SPSPDGYGDAYVWAISLGSALLGFAAVLILHRLARRLYGEPAATIAALVAWLASPLVF
                     YMYSHPAMAHAADAFANALAFAAWFAVCRDPRPRNWLLMGASIGLAALARTQNVLIVL
                     VPGVSLLALVPTAFRSGEGHTWLQSAAAFGLSALIAFAPQMIVWRVVYGSWIELNPYA
                     YTQGAGHFTGIFQVLPVLFSTDRGLFVWSPALLFAMAGLAPLYAQHRRLAAFLIVHFV
                     TQALLVSAWSVYNGALAFGARLLLQNTPTYVIGLTAFVDWLRRQGWTARRIAAIGAGF
                     VAWNFLLIVQYSVGTVPRTGAFPIGDLIAGQFTVLPTQFGRILTALLTRQ"
     gene            4219..5358
                     /locus_tag="AC162G_05545"
     CDS             4219..5358
                     /locus_tag="AC162G_05545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5075309.1"
                     /translation="MTRLRIVLDGRTISDHFPGIGRYAHRLAIALASAGEVDLTLLHN
                     ASQPNTHLPFDALPAGGVRLYPTEIAPFSVAEQTRLPALARRLRPDVWHAPYYVMPYR
                     RLSCPTIVTIYDVIPLALPQFWPARQRLIFSLAHRLALRAARRVIAISESTRRDVIRR
                     FHVDPGRVAVTPLAADERFRPQPESEVRRVRQAYTLPERFILYVGINKPPKNLPRLVD
                     AYAQLVAGSAELDFDCVIAGAWDNRYPESRERAAHLGLGDGVRFLGSIPDADLPALYA
                     AADLFVMPSLYEGFGLPVLEAMACGTPIACSDTSSLPEVAGDAGLFFNPYDIDQMAST
                     MLRAMTDPALRAELRVRGLERARLFSWERVARQTVDVYRAAAQAT"
     gene            5393..>5598
                     /locus_tag="AC162G_05550"
     CDS             5393..>5598
                     /locus_tag="AC162G_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075310.1"
                     /translation="MVSSMLRRIYVLTMWHNLRQRPAWVAGVVFLAALAVYVPTLAPG
                     LLWGGGDFATFQTKAFTRQIESNV"
BASE COUNT          871 a         1860 c         1720 g         1147 t
ORIGIN      
        1 ctccttgcga cgcgtctcac ccaataccat caactccatc actgtgagca caacgaccct
       61 agacgcatac tttgctccgc tcaacattgg tccgcatgta attaaattag acatcgaagg
      121 cgccgaattg ctcgcgctac gaggagcaga gggcgtgctg cgcacctttc gccctattct
      181 ggtggtggaa gcatcaaagg agctcatgca tgctttcggc tatggttgtg gcgacctgat
      241 agacttcctg cgtgcagtgg actaccacct ctacacactg aactattctg ctttgaagcc
      301 tattccgacg gagtaccgat ccggttacga gaacctcatc tgtctaccgc gcgattcatc
      361 gtcctatacg gcattgactt cgagcggtat ccggcctatt tgagccaatg agcactgcgc
      421 gcatcgcctt cgtcgatcac gccccgttcg tcggcggcgc agagacggta ctgctcgatc
      481 tgatcgggca tctggacagg tcccgcttcg atccgatcgt cttcctgccc gcccgctcgc
      541 cgctcgtccg gcatctcgac gcgcgcggcg tcccctggcg aaacctgccc atgccgcgca
      601 ttaacgtgag gtcgccgacc gcactgctgc gctggcggcg ggcgtccagc gagctggccc
      661 gactcgcccg cgactgcggc gtcgatcttg tacactcgaa caccgtgcgt gcacaccttg
      721 ccagcgcgtt cgtgcgcgat gtgccggtcg tctggatgct gcacgacgac acgttcccgc
      781 attggttgtt tcgccggctg cgccgtcgtc cggcgcggct gatcgccaat tcgtacacga
      841 ccgcaagcac ctacaacctg gaaggcgatc cgcgggcctg cgtcgtccac aacggcgtcg
      901 atacgcaggc cgagccgggg aatcgctacc gtttccggcg gcggatcggc atcgggtcgg
      961 acaaagtcct ggtggcgcac gtcggacgac tggtgcgctg gaaggggcag catcttttca
     1021 tccgcgcggc ggcgcgcgtc gccggagagc acccgcaggc acactttgcg ctcgtcggca
     1081 cgtacaccga aggcgacgac tcgccaggcg tcccttcggc tttgctcagg gcagggctga
     1141 gcggcggagc ggcgtatcac gccgagctgc agcggctggc tcaatcggcc gggttgggca
     1201 gccggttgac cttcgccggg catcaggata acatcgccga cgtgtacgcc ggcatcgatc
     1261 tgctggtgca cgcttcgacg cgccccgagc cgttcggacg cgtgctgatc gaggcgatgg
     1321 cggccgggtt gccggtgatc gcctcggcac agggcggccc gacggagatc gtcgaagatg
     1381 gcatcaccgg cctgctcgtc acaccgggca gctcgcaggc gctggcggag gccctgacga
     1441 agctgctggc cgatgcatcg cgtcgcgcgg aaatgggcgt cgccgcccgg cggctagcga
     1501 tcgagcgttt cgatattcgt actcaggtgg cgcgcatcga atcgatctac ctcgacatcc
     1561 tggccaaccg cgcacgcgtc gcagatgcga aacgcggcta aagtgacgaa ttacgtatga
     1621 aaatcgctgt gagatcttct ccgaccgtgt ctctcgtcat ggcaccgctg ctgctggcgg
     1681 gatacttgat ctggggcggc tgggcgatca cccacgataa gccgatcgat ttttatgcat
     1741 actacgctgc tgccgatgca ctcaatcgag gacaaccgct ttaccacgcc aacccggaac
     1801 aggaagcgct caacccgatt ccgggcctgc ctggcatcct gccaccgtac atttacccac
     1861 ccgcgctcgc ttccctgctg cggccgttag cacggctcac gccgccagtg gccgcgttcc
     1921 tttggatgat cctcaacagc gtggggcttg tgttgggcgc gtggatattg ggcacttcac
     1981 tagacgacga gcgcggcaga caagtggcac tgctggcgat ggccgggttc gtgccgccgt
     2041 tgacctcgct gtacgccggg cagttcaacg ggctggtctg gctgggcctg gcggccattt
     2101 tccacctggg cattgggccg cgacaattcg ggatgaacgg ccagccctgc cctgagcaaa
     2161 gccgaaggga cgccgggcga aggtctatcg ccgctgcgct cagcggcatc attttgggag
     2221 ctacgatact cctgaagccg ctgctctggt caagtgcgat cttcttcgcg tggcagcgcc
     2281 gctggctcgt cgtgctcact tgcatagccg gtacactgct gctcgtcctc gtcagcgcgg
     2341 ccttcgatgg gcttactccc tggcgagaat ttctgtccat cgcgccgcag ctcagctcga
     2401 cgacaaccac cgcttatccg cccaaccagt cactgtacgg tttcgcgctg cgcgctttca
     2461 ctacgcacga gtacggcgcg tcgctcgcgc tccaaccggc ctgggccgcg cctttcgccg
     2521 ctctgctgtc gctggcattg gtcggcgcca cgatggcgtt aacctggaac cgctccggcg
     2581 cacagcagcc ggcccgtttc gctctctggc taatcacagg cctcctggtg accccactta
     2641 gctggctcca ccacttgctg ctggcgctgt tgccgatcct catcatcggc acggtggcgc
     2701 tgaagcatcg ggcttggggc gcgctggccg catgtggcct ggcttacctt ctcatcgatg
     2761 tacagggcct ggcctggcat tcgctggtcg gcaacacact gttactttcg ctgggtacat
     2821 acggcatgtt gatcctctgg tcgattctag cggtctggct ggtgcgaaat aaactagagt
     2881 gaatgtgatc gtgatgcgtg ctttcattcg tgaacgacga ctgctcattg tcgtcttcat
     2941 cgtactcttc ccgctgctct ttcgtccgcg cgtgtatggc ttcgatccgg tgggctactt
     3001 ttcgtggctg cgctcggccg tcatcgacgg caatctcgat acgacggacg agtacctgcg
     3061 ctatggcaac gaaaaggtcg tcggcccgac ggtcaccggc tacaatcaca atccgtacgc
     3121 gatcggcgcg cctctgctgt ggtcgccgtt ctacctggcg gcgcacgcgg tcgaatcggg
     3181 gcgctcggcc atcacgcagt cgccttcacc cgacggctac ggcgacgcat acgtctgggc
     3241 catcagcctc ggatcggcgc tcctcggttt cgccgctgtg ctcatcctgc acaggctggc
     3301 gcggcgtctg tacggcgagc cggcggccac gatcgccgct ctcgtggcat ggctggcgtc
     3361 gccgctggtg ttctacatgt acagccaccc ggcgatggcg cacgcggccg atgcgttcgc
     3421 caacgcgctc gcgttcgcgg cgtggttcgc cgtatgccgt gaccccagac cgcgtaactg
     3481 gctgttgatg ggcgcgtcga ttgggttggc cgcgctggcg cggacccaaa acgtgttgat
     3541 cgtcctcgtg cccggcgtct cgctgttggc gctggtacct accgcatttc gatccggtga
     3601 aggccatacc tggctgcaat cggccgccgc tttcgggctg agcgcgctga tcgctttcgc
     3661 gccgcagatg atcgtgtggc gcgtcgtgta cggttcatgg atcgagctca acccatacgc
     3721 ctacacgcag ggcgccggcc acttcaccgg catatttcaa gttctgccgg ttctgttctc
     3781 taccgatcga gggctgttcg tatggtcgcc ggctctgctg ttcgcgatgg caggattggc
     3841 tccgctatac gcgcagcatc gccggctggc cgcctttctg atcgtccact ttgtcacgca
     3901 agcgctactg gtgagcgcgt ggtcagtgta caatggcgcg ctggccttcg gcgcgcgact
     3961 gctgctgcaa aacacgccga cgtacgtgat cgggctgacg gcgttcgtcg actggctgcg
     4021 ccgccaaggg tggaccgcgc gccgcatcgc cgccatcggc gccggcttcg tcgcatggaa
     4081 cttcctcctg atcgtgcagt attcggttgg cacagtgccg cgcacaggcg cgtttccgat
     4141 cggcgatttg atcgcgggcc agttcaccgt gctgcccaca cagttcggtc gcatcctgac
     4201 ggcgctgctc acgcggcaat gacccgtctg cgcatcgtcc ttgacggacg cacgatcagc
     4261 gatcattttc ccggcatcgg gcgctacgcg caccggctgg caatcgcgct ggctagcgcc
     4321 ggcgaggtcg atctcacgct gctgcacaat gcgtcgcagc ccaatacgca ccttccgttc
     4381 gatgcgctgc cggcaggcgg cgtcaggcta tacccgaccg agatcgcgcc tttctctgtc
     4441 gccgaacaga cgcgactgcc tgcgctcgcc cggcgactca ggcccgacgt gtggcacgcg
     4501 ccgtattacg tcatgccgta tcggcgcctg tcttgcccca cgattgtgac gatctacgac
     4561 gttatcccgt tggcgctgcc ccaattttgg cccgcccgtc aaaggctgat cttctcgctg
     4621 gcgcaccggt tggcgctgcg cgccgcgcgg cgcgtcatcg cgatttccga atcgacgcgg
     4681 cgtgatgtca tccggcgctt tcatgtcgat ccaggccgtg tcgccgtcac acccctcgcc
     4741 gccgatgaac gcttccggcc ccagcccgag tccgaggtgc ggcgcgtccg gcaggcttac
     4801 actctgcccg agcgcttcat tctgtatgtc ggtatcaaca aaccaccgaa gaacctgcct
     4861 cgactcgtcg acgcttatgc gcaactcgtc gccggatcag ccgaactgga tttcgattgc
     4921 gtgatcgcgg gcgcgtggga caatcgttac ccagagagcc gcgagcgcgc cgcgcattta
     4981 ggtctaggcg atggcgtacg ctttctcggc tcgattcccg acgccgatct cccggcgctg
     5041 tacgccgccg ccgatctatt cgtcatgcca tccctctacg aagggtttgg cctgccggtt
     5101 ctggaggcga tggcgtgcgg cacaccgatc gcatgcagcg acacgtccag cttgccggag
     5161 gtagccggcg acgccggtct cttcttcaat ccctatgaca tagaccagat ggcatcgacc
     5221 atgctgcggg cgatgaccga cccggccctg cgagcggagt tacgcgtgcg cggcctcgag
     5281 cgcgcccggc tgttcagttg ggaacgcgtc gcacggcaga ccgtcgacgt gtatcgagcc
     5341 gcagcgcaag cgacgtgatg cgagtgcaga cagacgccgg agcaatcgcc gaatggtctc
     5401 ttcaatgctg aggcgcatct acgtcttgac catgtggcac aacttgaggc agcggccggc
     5461 ctgggtggcc ggcgtggtgt ttctcgccgc gctggccgtc tacgtgccga cgctggcgcc
     5521 cggcctgttg tggggcggcg gtgacttcgc cacgtttcag acgaaagcct tcacccgcca
     5581 gatcgaaagc aacgtctt
//
LOCUS       DBNHXB010000143         5088 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_869782, whole genome shotgun sequence.
ACCESSION   DBNHXB010000143 DBNHXB010000000
VERSION     DBNHXB010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_869782"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="AC162G_05555"
     CDS             <1..651
                     /locus_tag="AC162G_05555"
                     /inference="COORDINATES: protein motif:HMM:NF016983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HIR5075311.1"
                     /translation="GIEAALNVLDEQASRMIKATASTVFVSTAISQNGRLDALMVLIA
                     QSRMVWQLAHLYYQRPSLPELIQLYANIGATAFLVSQIEDLDISEQVEPVIASALGGS
                     LASAAPGMNMVATLVTQSILEGTANAFLTLRVGVIGQRYCASLTTFDRRAVRRYASVT
                     AASMLGAIVSSSSMVVSKAILSAAKRAGVGTVGSVTGRIRTLGGRLNPFKKAARSR"
     gene            648..1679
                     /gene="hemH"
                     /locus_tag="AC162G_05560"
     CDS             648..1679
                     /gene="hemH"
                     /locus_tag="AC162G_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR5075312.1"
                     /translation="MTTGILLVNTGSPDSPSVEDVRAYLDEFLMDGRVLDFPYPIRRL
                     IVSLFILPSRPRHSAEAYRSIWGDEGSPLIVISRRVQQKLQDRIDAPVALAMRYGHPS
                     VQQGLQELLDRRVDWVLLVPLFPHYAMSTYESIVVAVRRTLSRLSPETLLDVLPPFYD
                     NPLYIDALVASARDHLAEGFDHLLFSYHGLPERHLRKTDPTGKHCLAVPDCCNVASPA
                     HATCYRAQVFRTTQAFVRRAGVPEGKYSIAFQSRLGRDRWLLPATGAELARLASGGVK
                     KLLLICPAFVSDCLESLQEIGIDGREAFLGAGGLELRLIPCLNDHPTWIGALAQWCEH
                     RIAQASLTP"
     gene            1676..2884
                     /gene="lhgO"
                     /locus_tag="AC162G_05565"
     CDS             1676..2884
                     /gene="lhgO"
                     /locus_tag="AC162G_05565"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HIR5075313.1"
                     /translation="MTSQRYDVAIIGGGIVGLATALALASRARVSLVVLEAEAQLAAH
                     QTGHNSGVIHSGLYYKPGSLKARNCVEGRQALYRFCEEHGIAHELCGKIVVATSEDEL
                     PALNELERRGRENGLNGMRRLRGEEIRDYEPHAGGIAGLHVPETGIVDYVQVAQTVAS
                     LVWQTGGEVRLRSRVRRFHRAADALALETDSGTVDCRNLINCGGLHSDRIARMCGVDP
                     GLRIVPFRGEYYELISNRRSLVRNLIYPVPDPRFPFLGVHFTRMIHGGVAAGPNAVLA
                     FRREGYRRSSFSARDTLSLLGHSGFWRMARRYWRMGLGEFYRSLSKRAFVKALQRLMP
                     ELRVEDVRPAGAGVRAQALEPGGSLVDDFRIIETGRMIHVLNVPSPAATASFSIGRTI
                     ADMAVRDFGL"
     gene            complement(2879..3568)
                     /locus_tag="AC162G_05570"
     CDS             complement(2879..3568)
                     /locus_tag="AC162G_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5075314.1"
                     /translation="MRHKILVVDDDATIRTALEVFLRESGYEPYTAANAGEGLRLLYS
                     EHPSLVLLDIMLPGIDGWEMATRIRELSDVPLIIISGRSDEADKLRGFRLGADDYVTK
                     PFSLIELGARIGAVLARAEKFARARERRQFTFDDLTVDLDRHRVTRGDKIINLTPTEF
                     RLLQVLIEKAGDAVSEEYLHQEVWGGVQKPGAGYVRRYIWFLRQKIERDPAHPDLIHT
                     VRKYGYRFGPL"
     gene            3807..4571
                     /locus_tag="AC162G_05575"
     CDS             3807..4571
                     /locus_tag="AC162G_05575"
                     /inference="COORDINATES: protein motif:HMM:NF016300.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR5075315.1"
                     /translation="MFNNSSKWYFLVGGLTVLAIVAGVILSGILNASATSTQAGQAGQ
                     TSEAGSPRTITAVGTGSASASPDVATAQIGVETQSASPEEATRENEERMKAVLEALEA
                     AGVDEKDIHTAYYNLWAEQRYDPEKGQPTGEYIYHVSNSLSVKIRALSEVGAILSEAV
                     EAGANNISGVAFSIEDTSALEMEAREKAVADAKARAESLAQLSGVELGEVVLVSEVIG
                     GPVPVVMERAMGGGGVSIEPGQLEVSMQVQMSFAIE"
     gene            4880..>5088
                     /locus_tag="AC162G_05580"
     CDS             4880..>5088
                     /locus_tag="AC162G_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075316.1"
                     /translation="MTTASIEPPSPAARGSRNRTLLIVALIAAVGVVAIGALALLSNQ
                     PPPSLTAELPPVVVDRAPERGEEQGA"
BASE COUNT          912 a         1615 c         1627 g          934 t
ORIGIN      
        1 ggcatcgaag ccgcgctcaa cgttctggac gaacaggcca gccggatgat caaagcgacc
       61 gcgtcgaccg tgtttgtgag tacggcgata tcgcaaaatg gccggctcga cgcgctgatg
      121 gtgctcatcg cccagtcgcg catggtctgg caattggccc acctgtacta tcaacgcccg
      181 tccttgcccg agctgattca actgtacgcc aacatcggcg cgacggcctt cctggtcagc
      241 cagatcgagg acctggacat cagcgaacaa gtggagccgg tgatagcttc tgcgctcggc
      301 ggttcgctgg caagcgcagc gcccggcatg aacatggtcg ccacgctcgt gacgcaatcc
      361 attctagagg gaacggcgaa cgcgttcttg acgctccggg ttggcgtgat cggccagcga
      421 tactgcgctt cgctgacgac gttcgatcga agagccgtgc gccgctatgc gagtgttacg
      481 gcagcgtcta tgctgggcgc gattgtcagc tcgtcgtcca tggtcgtctc aaaggctatc
      541 ctgtccgccg cgaagagggc cggtgtcgga acggtcggat cggtgaccgg cagaatccgc
      601 accctgggcg gccggctgaa tccgttcaag aaggcagctc gatcgcggtg acgacaggca
      661 ttcttctcgt taacaccggc tcacccgatt ctccctccgt cgaggacgtt cgtgcgtatc
      721 tcgacgagtt cctcatggat gggcgggtgc tggacttccc ctatccgatc cggcggctca
      781 tcgtcagcct cttcatcctg ccctcgcgac ccagacattc cgccgaggcg taccgctcga
      841 tctgggggga cgagggatcg ccgctgatcg tcatcagccg gcgtgtgcag cagaaactcc
      901 aggaccgcat cgacgcgccg gtcgcgctgg cgatgcgcta cggccatccc tcggttcaac
      961 agggtctgca ggagctgctc gaccgccgcg tcgactgggt tctgctggtg ccattgtttc
     1021 cgcactacgc aatgtcgacg tacgaatcga tcgtcgtagc cgttcgccgg acactgtcgc
     1081 gcctcagccc cgagaccttg ctcgacgtcc tgccgccctt ttacgacaat ccgctgtaca
     1141 tcgacgcgct ggtcgccagc gcgcgagatc accttgccga aggtttcgac cacctgctgt
     1201 tcagttacca cggcctgccg gagcggcatc tgcgaaaaac ggacccgacc ggaaagcact
     1261 gcctcgccgt tcctgattgc tgcaatgtcg catcaccggc gcacgcgacc tgctatcgcg
     1321 cccaggtgtt caggacaacg caggcgttcg tgcgtcgagc cggcgtgccc gaagggaaat
     1381 actcgattgc ctttcaatcg cggctcggcc gtgacaggtg gctgctgccc gccacgggcg
     1441 ccgaattggc ccgcctggct tccggcggcg tcaagaagct cctgctcatt tgcccggcgt
     1501 tcgtgagcga ctgcctcgaa tcgttgcaag agatcggcat cgacggccgc gaggctttcc
     1561 tcggcgccgg cggcctcgag ctccggttga tcccctgtct caacgatcat cccacgtgga
     1621 ttggcgcgct cgcgcagtgg tgcgaacacc gcatcgctca agcctccctg acgccatgac
     1681 ctcgcagcga tatgacgtag ccatcatcgg cggcgggatc gtcggcctgg cgacggcgct
     1741 ggcgctggcg tcgcgcgctc gcgtctcgct cgtcgtgctc gaagccgaag cgcaactggc
     1801 ggcgcaccag accggccaca acagcggcgt gatccactcc ggcctgtact acaagcccgg
     1861 ctcgctcaag gcgcgcaact gcgtcgaagg ccgtcaggcg ctgtatcgat tctgcgagga
     1921 gcacggcatc gcgcacgagc tctgcggcaa gatcgtcgtg gccacaagcg aagacgagtt
     1981 gcccgcgctc aacgagctgg aacgccgtgg gcgcgagaac ggattgaacg ggatgcgccg
     2041 gcttcgcgga gaggagattc gggattacga gccgcacgcg gggggcatcg ccgggctgca
     2101 cgtccccgag acgggcattg tcgattacgt ccaggttgcg cagacggtcg ccagcctggt
     2161 gtggcaaacc ggcggcgaag tgcggctgcg ctcgcgggtg cggcgcttcc accgcgcggc
     2221 agatgcgctt gcgctcgaaa ccgatagtgg gacggtggac tgccgcaatc tcatcaactg
     2281 cggcggcttg cactcggatc gcatcgcgcg catgtgcggc gtcgatcccg gcctgcggat
     2341 cgttcccttc cgcggcgagt attacgagct aatctccaat cgccgatctc ttgtgaggaa
     2401 tcttatctac cctgtccccg acccccgctt tcccttcctc ggcgtccact tcacgcgcat
     2461 gatccacggc ggcgtcgcag ccggcccgaa tgccgtcctc gccttcaggc gtgagggcta
     2521 ccggcgatcg agcttctcgg ctcgtgatac gctgagcctg ttgggccata gcgggttctg
     2581 gcgcatggcg cgccgctact ggcgcatggg gctgggcgag ttctatcgct cactcagtaa
     2641 gcgggcgttc gtcaaggcgc ttcagcgcct gatgcccgag ctgcgcgtcg aggacgtgcg
     2701 tccggccggc gccggagtcc gtgcgcaagc gctcgagccg ggcgggtcgc tcgtggacga
     2761 tttccgcatc atcgaaacgg gccggatgat tcacgtgctg aacgtgccat ccccggcggc
     2821 cacggcctcc ttcagcatcg ggcgaacgat cgcagacatg gccgtgcgag acttcggcct
     2881 atagaggtcc gaagcggtat ccgtacttcc gcacagtgtg gatcaggtcg gggtgggccg
     2941 ggtcgcgctc gatcttttgg cgcaggaacc agatgtagcg ccgcacgtat ccggcgcccg
     3001 gcttctgcac gccgccccat acctcctggt gcagatactc ctccgagact gcgtcgccag
     3061 ccttctctat caggacctgc agcagtcgaa attcggtcgg cgtcagattg atgatcttgt
     3121 cgccgcgcgt gacgcgatgc cgatcgaggt cgacggtcaa atcgtcgaag gtgaactgcc
     3181 ggcgctcgcg ggcgcgcgcg aacttctcgg cgcgggccaa cacggccccg atccgcgcgc
     3241 ccagctcgat caggctgaac ggcttggtga cgtagtcgtc cgcgccgagc cggaacccgc
     3301 gcagcttgtc ggcttcgtcg gaccggccgc tgatgatgat gagaggcacg tctgatagct
     3361 cgcgaatgcg cgtcgccatt tcccagccat ctatgccggg cagcattatg tcgagcaaca
     3421 ccaggcttgg atgctcgctg tacagtaagc gcagaccttc gccggcgttg gcggcagtgt
     3481 acggttcgta gccggactcc cgaaggaaaa cctctagcgc cgtgcgaatg gtcgcgtcgt
     3541 cgtcgacaac caagatttta tgtctcataa cacgtacggt cttgttgcgc ccgtcagtgc
     3601 gcccgattta cacaggagta tatcatggca ggcttcagca gtgagtagga gtttagtcga
     3661 tttagacaaa taccataccg aattcatatc ttaatataac ataattttga cattgtgaaa
     3721 caagttacgg aaatggaact tgaacgcagt ccgtgtcgtc tcaaggtaga aatctgaacg
     3781 cactgcagtg tgcgatggag ggagcaatgt tcaacaatag cagcaagtgg tatttcctgg
     3841 tcggcggcct caccgtgctg gccatcgtcg cgggcgtcat tctcagcggg atactgaatg
     3901 catcggctac ctcgactcag gccggccaag ccggtcagac gagcgaagcc ggctcgccgc
     3961 gcaccatcac ggccgtcggc accggctcag cctcggcgtc ccctgacgtg gcgacagcgc
     4021 agatcggcgt cgagacacaa tcggcctcgc ccgaagaggc gacgcgcgag aacgaagaac
     4081 ggatgaaggc agtcctcgag gctttggagg ccgcgggcgt cgatgagaaa gacatccaca
     4141 cggcgtatta caacctgtgg gccgagcagc gatacgatcc ggagaaaggc cagcctaccg
     4201 gcgagtacat ctaccacgtc agcaatagcc tgtctgtgaa gatccgcgcc ctctcggaag
     4261 tcggcgcgat cctgagcgag gcggtggagg ccggcgcgaa taacatctcc ggcgtggcct
     4321 tcagcatcga agacacttcg gcgctcgaga tggaagctcg cgagaaggcc gtggccgacg
     4381 cgaaagcacg cgcggagagc ctggctcaac tgagcggcgt cgagctgggc gaggtggtgc
     4441 tcgtcagcga ggtcatcggc gggcctgtgc cggtcgtcat ggagcgagca atgggcggcg
     4501 gcggggtgtc catcgagccg ggccagctcg aggtgagcat gcaggtgcag atgtccttcg
     4561 cgatcgagta aggcagaaga ccagccaggt ctcaaagacc tggcaagtct gcggataaac
     4621 ggatagaaga cctgccaggt ctcgaagacc tggccggtct gcgtttccgc ctggagagcg
     4681 tcgtgtttgc gtcgggcaaa cgtcgggcga tccgctcgaa cgaatgcgac gataccggtg
     4741 tcttataggc agggctcgcc cgcgcgggca cttactcctt gccacaagcc cgtatgggcg
     4801 ttacaatcgc cgtgcaccgc acgatgagcg gacagatggg ggggcgcgcc atcgtgcggg
     4861 cacaaagggg gacattccca tgaccactgc ttccattgag cctcccagcc cggcagcgcg
     4921 aggctcacgc aatcgaactc tcctgatcgt ggcgctcatc gccgccgtgg gcgtcgtcgc
     4981 cattggcgcc ctcgcactgc tgtccaatca accgccgcct tcgctcacgg ctgagctgcc
     5041 gccggtcgtc gtggaccgtg cgccggagcg cggcgaggaa cagggggc
//
LOCUS       DBNHXB010000144         8047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_870552, whole genome shotgun sequence.
ACCESSION   DBNHXB010000144 DBNHXB010000000
VERSION     DBNHXB010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_870552"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1791
                     /locus_tag="AC162G_05585"
     CDS             <1..1791
                     /locus_tag="AC162G_05585"
                     /inference="COORDINATES: protein motif:HMM:NF025925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HIR5075317.1"
                     /translation="VRRRSTIRLSQADIEAGYALACQTIIEGDAAILVPEQEKVERRL
                     VTDKTARAVALPFPYDCARDQTVRAFFVALDEPTLADAVDDLARLERGLAAHGVRDLD
                     VPLPLMREIGARLREANWQVTAVVETDNWRRPDGPPRLIDLLAGDRREAVWGVAIDIG
                     TTTVSVYLVNLLTGEAVDSAAEYNAQISRGEDVISRIIYAGRNGGLGELQSLVRGTID
                     EVIDRLAQRNHVDPKRIYKATVAGNTTMMHLFLSVPPATIRLTPYIPAINHPVPLLAS
                     EVGLNIHPLATVDSLPGVASYVGADITAGVLSSGLADAEELTLFIDVGTNGEIVLGSQ
                     DWLVTCACSAGPAFEGAGVVCGMRATRGAIEEVWINGSTYEPTVRVIGETKPRGICGS
                     GLIALVAELFITGVVDKGGKFKMDLNTPRLREGDHGPEYVVAWANETATGRDIVVTKV
                     DVDNLMRAKAAIYAGFTVLAQSVGVDLADVERVLVGGAFGKYINVEKAVQIGLLPDLP
                     WDRFQFLGNTSVMGAYMALASREARARIRSIARKMTYVELSADNTFYDAFTSALFLPH
                     TDASRFPTVEALWNNGRDRGDRGDGEAEST"
     gene            1801..2607
                     /locus_tag="AC162G_05590"
     CDS             1801..2607
                     /locus_tag="AC162G_05590"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR5075318.1"
                     /translation="MRRSMILLVVSLVLLAVCAASSQPLTSFGLDSMVAVPAGWFWMG
                     QDDGPESNRPQHAVYLDAFAIDRTEVTMAAFTEFVAATGYQAKGWEPPLRLPPLAGGA
                     GGGPHADEPAAGIMWQDADAYCRWFGKRLPTEAEWEKAARGTDRRIYPWGDQWNADCA
                     NTADSGYGRVLPVGSFLDGASPYGALDMAGNVAEWVSDYFDAGYYAISPERNPTGPDI
                     VVDHVLRGGSWASPSAHAQTFFRDSSHSARRNPRVGFRCALSLARTSGAP"
     gene            2620..3555
                     /locus_tag="AC162G_05595"
     CDS             2620..3555
                     /locus_tag="AC162G_05595"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR5075319.1"
                     /translation="MLKVGENIHIISPRVKEALAGRDGAFFVELARKQKEAGADALDL
                     NIGPQKKAGPEVMDWLVDCVQEGVGDMTLSFDTTNLAAIEVGLKKVARNGQANAIINS
                     TSAEEERLATVPPLAARYGAKLIALCMEKSGIPVGADARVSIAMEKLIPRAQEVGVPM
                     DHLLIDPLILTVSGCQEYVPQAIEAVRMLKMVADPPPLTLVGLSNVSNAVPALMRPLL
                     NRTYVAMLMAAGVDAAILDPLDRELMEAIRIVEQRDESTPVGALYLKLFDAIAAGAEL
                     EPGDVDMGDSQQVEIWKTVQVLLNKVIYTDSYLRL"
     gene            3673..5031
                     /locus_tag="AC162G_05600"
     CDS             3673..5031
                     /locus_tag="AC162G_05600"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent malic enzyme"
                     /protein_id="HIR5075320.1"
                     /translation="MLKDVPTPTVEELLAKAKKPAEDAMRLHRFYHGKVETTLRCRVR
                     DLGDFAIWYTPGVAAPCKAIAADPELVYEMTHKWNTVAVVSDGTRVLGLGDIGPKAGL
                     PVMEGKALLYKYLGGVDAWPIMLDTKDPDKIIEAVLLIQPGFGGVNLEDLSQPKCFRI
                     LDTLREKAEIPIWHDDQQGTATVTLAGLLNALKIVGKKLSEARIVFVGTGAANVACSR
                     LIFASGGDPARCIMVDSRGILNKNRRDIELRRAEFVDKWRLCQITNEEQREGGIPEAL
                     KDADVVSALSTPGPDVLKKEWIAAMAKDAIVFACANPIPEIWPWDAKEAGAAVVATGR
                     SDFPNQVNNSLGFPGIFRGTLDVRANTITDEMCQAAAQALADMAVERGLTSDHILPSM
                     DDWDVFPREAAAVAVKAVEQGIARRQVTYEEEYHHAAGIIRRARDITHTMMKDGYVEE
                     GD"
     gene            5034..5969
                     /locus_tag="AC162G_05605"
     CDS             5034..5969
                     /locus_tag="AC162G_05605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5075321.1"
                     /translation="MYDLIILGGGPAGLTATIYALRKRLNVLLITKDLGGKTNYRLQL
                     PDVEHHLVIRGEEVVNRFVNEIEYLDFARVMDRVDKIERTADGFSVFTRSGFDYTGRT
                     LIVATGANARGLDIPGEKEYMMRGLCYSAVSYAPLFVERNTAVIGDAGLALRAAAELA
                     QIASSVTLVAPTHGDLDSPLGRRLRASPNVLIMEGCKLNEIKGDEFARELVVQKNGER
                     HTIRADAFFVELALTPNSNLVKHLVELDEAGCIKIDQRNRTSTPGIFAAGDVTDAYAE
                     QVLIAIGEGAKAALSVYEYLLSLPVEEKVAAGEWR"
     gene            6000..6707
                     /locus_tag="AC162G_05610"
     CDS             6000..6707
                     /locus_tag="AC162G_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5075322.1"
                     /translation="MHVLLLADVHANWEALLGLQRAEPKPDAILFAGDAVGYGPDPLN
                     CARWLLTHTTLAVRGNHDQAMASAGVVGMPSELEDAALETLVYSRRLLTQRDQARVGQ
                     WPTSASIALGDARFYVTHGTPRDPLGGQLNPATCTEAELHALFDDIPADVIVLGHTHM
                     PAIRRFDGRLIINPGSLGQPRYGTPDATYAVWNDGRVQIKHLHYDHDEPARKLRLASL
                     SPEVIDQLCSVLETGMV"
     gene            6795..7652
                     /gene="panB"
                     /locus_tag="AC162G_05615"
     CDS             6795..7652
                     /gene="panB"
                     /locus_tag="AC162G_05615"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR5075323.1"
                     /translation="MPDRKKITLPDLMKKMRDGIPITMITCYDYSMAHLVEQAGVDMV
                     LVGDSLGMTMLGYEGTLPVTMDDMIRHTQAVRRGSPTSWLIGDMPYMSYQPSDETAVR
                     NAGRFMAETGCDAVKLEGGRQMTSRVQAIADAGIPVMGHLGLTPQSASALGGFRVQGK
                     GWEQALRIVEDARVLEEAGAFSILFEMIPDRVQQIITDRAGIPIISLGSGPHAHGQLL
                     IFHDMFGLYPRFKPKMAKVYGNAGEVILNGLKQYVTEVTSREFPQPEHWFGMKDEELE
                     ELKQRLGDQ"
     gene            7763..>8047
                     /locus_tag="AC162G_05620"
     CDS             7763..>8047
                     /locus_tag="AC162G_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075324.1"
                     /translation="MTDPLRERLRISEEHLNDINALLLDPDARVINAFLDVVSKYGTP
                     EEINRKAEEARKLPNLMARLKAIGSPYVDDLEWLIEQRDKGAFISEADYRR"
BASE COUNT         1538 a         2497 c         2686 g         1326 t
ORIGIN      
        1 gtccggcggc gctcgacgat ccgcctgtcg caggcggaca tcgaggccgg ctacgcgctc
       61 gcctgccaga cgatcatcga aggtgacgcg gcgatactcg tccctgagca agagaaggtc
      121 gagcgccggc tggtgaccga caagacggcg cgcgccgtcg ccttgccgtt cccatacgac
      181 tgcgcgcgcg atcagaccgt gcgggcgttc ttcgtcgcgc tcgacgagcc aacgctggca
      241 gacgccgtcg acgatctggc ccggctcgag cgcgggctgg ctgcgcacgg cgtgcgcgac
      301 ctcgacgtgc cgctgcctct gatgcgcgag atcggcgcgc ggttgcgcga agcgaactgg
      361 caggtgaccg ccgtcgtcga gacggacaac tggcgcaggc cagacggccc gccacggctg
      421 atcgacctgc tcgcgggcga tcggcgcgag gccgtgtggg gcgtcgcgat cgacatcggc
      481 acgacgacgg tgagcgtcta cctggtcaat ctgctgaccg gcgaggcggt ggacagcgcc
      541 gcagaataca acgcgcagat ctcgcgcggc gaagacgtta tctcgcgcat catctacgcc
      601 ggccggaacg gcggcttggg agagctgcaa tcgctggtgc gcggcacgat cgacgaagtg
      661 atcgaccggc tggcgcagcg caaccacgtc gatccgaagc gcatctacaa agcgacggtc
      721 gcgggcaata cgacgatgat gcacctgttt ctcagcgtgc cgcccgccac gattcgcctg
      781 acgccgtaca taccggcgat caatcaccct gtgccgctgc tggcctcgga agtagggctg
      841 aacatccatc cactggcgac ggtggacagc ctgccgggcg tcgcgtccta cgtcggggcc
      901 gacatcacgg ccggcgtgct gtcgtcggga ttggcggatg ccgaagagct tacgctcttc
      961 atcgacgtcg gcaccaacgg cgagatcgtg ctcggcagcc aggactggct cgtgacgtgc
     1021 gcctgctcgg ccgggccggc gttcgaaggc gcgggcgtgg tatgtggcat gcgcgcgacg
     1081 cgcggcgcga tcgaagaagt gtggatcaac ggttcgactt acgagccgac ggttcgcgtc
     1141 atcggcgaga cgaagccgag gggcatctgc gggtcgggcc tgatcgcgct cgtcgccgag
     1201 ctgttcatca ccggcgtcgt cgacaagggc ggcaaattca agatggacct gaacacgccg
     1261 cgcctgcgcg agggcgacca cgggccggag tacgtcgtcg cgtgggcgaa cgagaccgcg
     1321 accgggcgcg acatcgtcgt gacgaaggtc gacgtcgaca acctgatgcg cgccaaggcg
     1381 gcgatctacg ccgggttcac ggtgctggcg cagagcgtcg gcgtcgatct ggcggacgtc
     1441 gagcgcgtgc tcgtcggcgg agcgttcggc aaatacatca acgtcgagaa ggcggtgcaa
     1501 atcgggctgc tgcccgatct gccgtgggat cggttccagt tcctgggcaa cacgtcggtc
     1561 atgggcgcgt acatggccct ggcgtcgcgc gaggcccgcg cgcggatccg gagcatcgcg
     1621 cgcaagatga cctacgtcga gctttcagcc gacaacacct tctacgatgc attcacgtca
     1681 gcgctgtttc tgccgcatac cgatgcaagc cggttcccga cagtcgaggc attgtggaat
     1741 aacggccggg accgaggcga tcgtggggat ggtgaagcgg aatcgacttg accagatacc
     1801 atgcgccgtt cgatgatctt gttggttgta tcgctggtct tgcttgccgt ctgcgcggcg
     1861 agttcgcagc cgctgacctc gttcgggctg gactcaatgg tcgcagtgcc ggccgggtgg
     1921 ttctggatgg ggcaggacga cgggccggaa tccaatcggc cacaacacgc ggtttacctg
     1981 gatgccttcg caatcgatcg aaccgaagtg acaatggcgg ctttcaccga gttcgtggcg
     2041 gcgaccggct atcaggccaa aggctgggaa ccccccctcc ggctcccccc cctcgcgggg
     2101 ggagccggag gggggccgca cgccgacgag ccggcggcgg gcatcatgtg gcaggatgct
     2161 gacgcgtact gccgctggtt tggcaagcgt ctgccaaccg aggcggagtg ggagaaggcc
     2221 gcgcgtggca cggataggcg catctatccg tggggcgacc agtggaatgc ggactgcgcc
     2281 aacacggccg acagtggtta cggtcgcgtg ctgccggttg gcagtttctt ggacggcgca
     2341 agcccctacg gcgcactcga catggcgggc aacgtggccg aatgggtatc cgattacttc
     2401 gacgccggct actatgccat ctcgcccgag cgcaacccaa ccggcccgga catcgtagtc
     2461 gatcatgtgc tgcgaggcgg atcgtgggcg tcgccaagcg cgcatgcgca gacgttcttc
     2521 cgtgactcgt cgcactcggc gcggcgcaac ccgcgcgtcg ggttccgttg cgcgctgtcg
     2581 ctggccagga catccggcgc gccatgaaag gagacattca tgttgaaagt gggagagaat
     2641 atccacatca tctcgccgcg tgtgaaggag gcgctcgccg gccgcgacgg cgctttcttc
     2701 gtggagctgg cgcgcaagca gaaggaagcc ggtgccgacg cgcttgatct caacatcggg
     2761 ccgcagaaga aggccgggcc ggaagtgatg gattggctgg tcgactgcgt gcaggaaggc
     2821 gtcggcgata tgacactatc cttcgacacg accaacctgg ctgcgatcga agtcggcctg
     2881 aagaaagtcg ccaggaacgg gcaggccaac gcgatcatca actccacctc cgccgaggag
     2941 gagcggctgg cgacggtgcc cccgctggcc gccaggtacg gcgcgaaatt gatcgcgctg
     3001 tgcatggaga aaagcggcat ccctgtcggc gccgacgcgc gcgtctccat tgccatggag
     3061 aagctgatcc cgcgtgcgca ggaagtcggc gtccccatgg atcacctgct catcgatccg
     3121 ctgatcctga ccgtatccgg ctgccaggaa tacgtgcccc aagcgattga ggccgtgcgc
     3181 atgctcaaga tggtcgccga tccgccgccg ctcacgctgg tggggctgtc gaacgtgtcg
     3241 aacgcggtgc ccgccctgat gcgcccgctg ctcaaccgca cgtatgtggc gatgctcatg
     3301 gccgccggcg tcgacgcagc gatcctcgac ccgctcgacc gagaactcat ggaagccatt
     3361 cgcatcgtcg agcagcgcga cgagtccacc ccggtcgggg cgctctatct caagctgttc
     3421 gacgccatcg ccgccggcgc cgagctcgaa ccgggcgacg tggacatggg cgattcgcag
     3481 caggtcgaga tctggaagac cgtgcaggtg ctgttgaaca aggtgatcta taccgactcg
     3541 tacctgagac tgtaaccggt tgtcagtaat cagttgtcag taatcagtta tcagtaatcg
     3601 gtcgtcagtg ttcactgctt actgttcact gatcaccgat cactgttcac tgaccccaaa
     3661 ctggaggcca caatgctcaa agatgttcca accccaaccg tcgaagagct gctcgccaag
     3721 gcgaagaaac ccgccgaaga cgcgatgcgg ctgcaccgtt tctaccacgg caaggtagag
     3781 acgacgctgc gctgccgagt gcgcgacctc ggcgatttcg ccatctggta cacgccgggc
     3841 gtcgccgcgc cgtgcaaggc gatcgccgca gacccggagc tggtttacga gatgacgcac
     3901 aagtggaaca ccgtcgccgt cgtctcggac ggcacgcggg ttctcggcct gggcgacatc
     3961 ggccccaagg ctggcctgcc cgtcatggag ggcaaggcgc tgctgtacaa gtatctcggc
     4021 ggcgtagacg cctggccgat catgctcgac accaaagacc ctgacaagat catcgaggcc
     4081 gtgctgctga tccagcctgg cttcggtggc gtcaacctgg aagacctgtc gcagcccaag
     4141 tgcttccgca tcctcgacac gctgcgcgag aaggccgaga tcccgatctg gcacgacgat
     4201 cagcagggca cggcgaccgt cacgctcgcc ggcctgctca acgcgctcaa gatcgtgggc
     4261 aagaagttgt cggaggcgcg catcgtgttc gtcggcactg gcgcagcgaa cgtcgcctgc
     4321 tcgcgcctca tcttcgcctc cggcggcgat cccgccaggt gcatcatggt ggacagccgg
     4381 ggcatcctga acaagaaccg gcgcgacatc gagctgcgca gggccgagtt cgtcgacaag
     4441 tggaggctgt gccagatcac gaacgaagag cagcgcgagg gcggaatccc tgaagccttg
     4501 aaggacgccg acgtcgtgag cgcgctctcg acgcccggac cggatgtcct gaagaaggaa
     4561 tggatcgcgg cgatggcgaa agacgcgatc gtgttcgcct gcgccaaccc gatccccgag
     4621 atctggccgt gggacgcgaa agaagcaggc gcagccgtgg tcgcaaccgg ccgctctgac
     4681 ttccccaatc aggtcaacaa ctcgctcggc ttcccaggca ttttccgggg gacgctcgat
     4741 gtgcgcgcga acaccatcac cgacgagatg tgccaggcgg ccgcgcaggc gctggccgac
     4801 atggccgtcg agcgcgggct gacatctgac cacatcctgc cgtcaatgga cgactgggac
     4861 gttttcccgc gtgaggccgc cgcagtggcg gtgaaagcgg tggagcaggg catcgcccgc
     4921 cgccaagtga cgtacgagga ggagtaccat cacgcggcag gcatcattcg ccgcgcgcgc
     4981 gacatcacgc acacgatgat gaaagacgga tacgtcgagg agggagacta accatgtatg
     5041 acctgatcat tctgggcggc ggcccggccg gattgacggc cactatctac gcgctgcgca
     5101 agcgcctgaa cgtattgctc atcacgaagg acctcggcgg aaagacgaac taccgcctgc
     5161 aattgcccga cgtggagcac cacctcgtca tcaggggcga ggaagtcgtc aaccgcttcg
     5221 tgaacgagat cgagtatctt gacttcgccc gggtgatgga ccgcgtcgat aagatcgagc
     5281 ggacggccga cgggttcagc gtgttcacac gcagcggctt cgactacacc gggaggacgc
     5341 tgatcgtcgc caccggcgcg aacgcgcgcg ggctggacat tcccggcgag aaagaataca
     5401 tgatgcgcgg gctgtgctac agcgcggtga gctacgcgcc gctgttcgtc gagcgcaata
     5461 ccgccgtcat cggcgacgcc ggcttggcgc tgcgcgcggc ggcagagctg gcgcagatcg
     5521 cgagcagcgt cacgctcgtc gcgccgacgc acggcgatct cgacagcccg ctgggccggc
     5581 gtctgcgcgc gtcgccgaac gtgctgatca tggagggctg caagctgaat gagatcaagg
     5641 gtgacgagtt cgcccgcgag ctggtggtgc agaagaacgg cgagcggcac acgattcgcg
     5701 ccgatgcgtt cttcgtcgag ctggccctga cgcccaattc caacctggtg aagcacctcg
     5761 tcgagctgga cgaagccggc tgcatcaaga tcgaccagcg caatcgcacc tcgacaccgg
     5821 gcatcttcgc cgctggcgac gtgaccgacg cctacgccga gcaggtgttg atcgcgatcg
     5881 gcgaaggggc gaaggcggcg ctgtcggtgt acgagtacct gctcagcctg ccggtcgagg
     5941 agaaggttgc cgccggcgag tggcggtagg cgaccgcgcg gcggccggtg tagaagcgta
     6001 tgcacgttct cttgcttgcc gacgttcacg ccaactggga agcgctgctc ggcctgcagc
     6061 gcgcggagcc gaagccagac gcgatattgt tcgcgggcga cgcggtgggc tacggcccgg
     6121 atccgctcaa ttgcgcgcgg tggcttctca cgcatacaac cctcgccgtg aggggtaatc
     6181 acgatcaggc gatggcctca gcgggggtcg tcgggatgcc atccgagctc gaggacgccg
     6241 cgctcgagac gctggtctac tcgcgccggc tgctcacaca gcgcgatcag gcccgcgtgg
     6301 ggcagtggcc gacctcggcg tccatcgcgc tgggcgatgc gcgcttctat gttacacacg
     6361 ggacgccccg cgatccgctc ggcgggcaac tgaatccggc aacctgcacg gaggctgagc
     6421 tgcacgcgct gttcgacgac attccggcgg acgtcatcgt gttgggccat acacacatgc
     6481 cggcgattcg ccggttcgac ggccgcctga tcatcaatcc cggcagcctc ggacagccgc
     6541 gatatgggac gcccgatgcg acgtacgccg tgtggaacga cggccgggtt cagatcaagc
     6601 acctgcacta cgaccacgac gagcctgcac gcaagctgcg gcttgcgtcg ttaagcccgg
     6661 aagttatcga tcagttgtgc agtgttttgg aaacaggaat ggtatagacc tggcaggtct
     6721 tgataaggtt tctccgaagt gtatcggaaa acgaagatct gccaggcctg gcccaccaga
     6781 aaacggaggc ttgtatgccc gacaggaaga aaatcacgct gcccgacttg atgaagaaga
     6841 tgcgtgacgg cattccgatc acgatgatca cgtgctacga ctattccatg gcccacctgg
     6901 tcgaacaggc cggggtggac atggtgcttg tcggcgacag cctgggtatg acgatgctcg
     6961 gctacgaggg cacgcttccc gtgacaatgg acgatatgat ccggcacacg caggcggtgc
     7021 ggcgcggctc gccgacgagc tggctgatcg gcgacatgcc gtacatgagc taccagccgt
     7081 cggacgagac cgccgtgcgc aacgcgggcc gcttcatggc cgagaccggg tgcgatgcgg
     7141 tgaagctcga aggaggccgg cagatgacct cgcgcgtcca ggcaattgcc gacgccggca
     7201 tcccggtgat gggtcacctc ggcttgacgc cgcaaagcgc gtccgcgctg ggcggttttc
     7261 gagtgcaggg taaaggctgg gagcaggcgc tgcgcatcgt cgaagacgcc agggttttgg
     7321 aagaggcagg ggcgttctcg atcctgttcg agatgatccc cgaccgcgtg cagcagatta
     7381 tcaccgatcg cgccggtatc ccgatcatca gcctcggttc cgggccgcac gcgcacggcc
     7441 aactgctgat cttccacgac atgttcggcc tgtatcctcg cttcaagccg aagatggcga
     7501 aggtgtacgg caatgccggc gaggtcattc tcaacggcct caagcagtac gtgaccgaag
     7561 tgacgagcag agaattccca cagccggagc attggttcgg catgaaggac gaggagctgg
     7621 aagaactgaa gcaacgactc ggtgatcagt gagcagtgat cagtgatcag taatcagtga
     7681 ccaccagtca ctgacaactg ataactggtg actaatcacc agtaaccaat aaccaataac
     7741 tgttcccttt acggaggtga acatgacaga cccactgcgc gagcgactgc gcatttccga
     7801 agagcacctg aacgacatca acgcgctact gctcgacccg gatgcgcggg tgatcaacgc
     7861 ctttctcgac gtggtgagta aatatggcac acctgaggag atcaaccgca aggccgagga
     7921 ggcgcgcaag ctgccgaatc tgatggctcg gctgaaggcc atcggttcgc cgtacgtgga
     7981 cgatctcgaa tggctgatcg agcagcggga caaaggcgca ttcatcagcg aggcggacta
     8041 tcgccgc
//
LOCUS       DBNHXB010000145         6104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2548099, whole genome shotgun sequence.
ACCESSION   DBNHXB010000145 DBNHXB010000000
VERSION     DBNHXB010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2548099"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="AC162G_05625"
     CDS             complement(<1..260)
                     /locus_tag="AC162G_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR5075325.1"
                     /translation="MPDKILVCVAWPYANGPQHLGHIAGAYLPPDIFARYHRLKGNDV
                     LMVSGSDSHGTPITVQADKEGVTPREVFERYHLLFLETYQQLG"
     gene            complement(466..1134)
                     /locus_tag="AC162G_05630"
     CDS             complement(466..1134)
                     /locus_tag="AC162G_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075326.1"
                     /translation="MKILSARWRLIIVAGLSVALVAACATPTPAPVPTEPPPSETPPP
                     THTNTPEPTSTPTDSPEPTSTPTAAPTDTPTSTSTPSRTPTPRATNTPRATATPTQPP
                     VPPLLSAARDMLNRVHALGGSMDRIYAGSGAEACAPFLADFYAIVNAATHDVNQQPSQ
                     VQSAYSLYREAVAIITDKVNSIREVCEAGGGTIGNLAFDVARIGVNEAGDRLNAAIGL
                     LQNP"
     gene            complement(1486..2847)
                     /locus_tag="AC162G_05635"
     CDS             complement(1486..2847)
                     /locus_tag="AC162G_05635"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008095599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HIR5075327.1"
                     /translation="MVSTFLVRNAQLLITMDDAGRTIPDGALYGRDNVIDWVGPARDL
                     PPAYLAADRVVDASGMVVLPGLVNTHHHLYQTLTRALAPDSGLFDWLKTLYPVWARMD
                     GEAVYISALTGMAELIMSGCTTSSDHLYLFPNGARLDDEIRAAQEIGLRFHAARGSMS
                     LGESKGGLPPDRVVEDEELVIRDCVRVIERYHDPKPYAMLRVVVAPCSPFSVTPDLMR
                     ESANLARQYGVTLHTHLAETKDEEAFCLEKFGHRPAMYAAELGWVGDDVWWAHSVHVD
                     EKEIGIMAGGGTGAAHCPTSNMRLASGIAPVRRMVDLGVKVGLGVDGSASNDGSHMLA
                     EARQALLLQRVTGNPRGLKAYEALWLATRGGAAVLGRDDVGELAPGKAADFIGFKLER
                     LDYAGALHDPLTALMFCAPQRVDLSVINGRVVVEDGELQTVDWRLVIEKHNRISKRMI
                     RGE"
     gene            complement(3223..4455)
                     /locus_tag="AC162G_05640"
     CDS             complement(3223..4455)
                     /locus_tag="AC162G_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5075328.1"
                     /translation="MSKTLLVLRYEISTAFRRRSFLFAVFGLPVILFLAFVVLSRIGE
                     SQPSGPTGSVSGPDMRKLQREGYVDLSGLIEEIHPDVPRGILTAYPDEDSARRAMQSG
                     EIAAYYLVPPDYLETGNLIYVNPNYRPVSAEGQAWVMRWTLFANLVGNDPERMRAGNA
                     MNVHVTALAPADMRQEVDSPLVFYIPYGMMMLSGMVLMMSAGLLLNSVSVEKQNRTME
                     ILLSSITSRQMLIGKVAALGILGLLQAAVWFGTAYFLLGAGGRVALPPGLELSPSIVF
                     WGIAFFVSSYAVYGSLMAGLGALASSRREASQFILLIIWPMLVSFVFSPMLPIEPHGA
                     LATGLSLFPLTAPFTMLARLVVGGVPWWQPWLAAALMAVTAVFIVRLVARMFHAQTLL
                     AGQPFSARLYLRALFGST"
     gene            complement(4525..5796)
                     /locus_tag="AC162G_05645"
     CDS             complement(4525..5796)
                     /locus_tag="AC162G_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5075329.1"
                     /translation="MRKMWGVATYEYRRLVFRRSFVLVVLSVPLMLASMIGLGWLIES
                     MENNSAPVGYVDHAGLLADPIPVPQAEGSASGPGSPEPVALIPFETEDAAHAALLSGD
                     IQAYYVVTENYFATNRVELVYVEPPGENATDQFWDFMQINRLAGLPPEVARRAVEGSY
                     LIVRWPEDMPGGGREFDDRMILGNFIPAFAGAAFIMLLLMLSGYLMSAVAEEKENRTM
                     EIVVTSISPNQLMAGKVLGISAIALTQVVAWNILIGLAIGVGERQLGISVLQGLSLDP
                     QLIAQLAIVGVPTFVTFAALMIALGATVAEAHEAQQLTGLFAVPMWAPYWLAIIIIQD
                     ANGPLATGLSFFPPTALSTLSLRIVFTPVPFWQIAASALVQVVCAAGAVWLAGRAFRL
                     GMLRYGQRLRWRELFAAGKRHATPLSAYPKT"
     gene            complement(5789..>6104)
                     /locus_tag="AC162G_05650"
     CDS             complement(5789..>6104)
                     /locus_tag="AC162G_05650"
                     /inference="COORDINATES: protein motif:HMM:NF025109.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein DrrA1-3 family
                     domain-containing protein"
                     /protein_id="HIR5075330.1"
                     /translation="LCDRILLINQGRNLLYGNLEEIRRRYVGDAVLVSAVGELPHLTG
                     VEAMTVVNHVTKLTLGNDSTPQDVLKALVERGVVLEKFEIAVPSLNEIFIRAVSEEVP
                     DA"
BASE COUNT         1186 a         1953 c         1869 g         1096 t
ORIGIN      
        1 ccgagctgct gatacgtttc gagaaagagc aggtggtagc gctcgaacac ctcgcgcggc
       61 gtcacgcctt ccttgtcggc ctgcaccgtg atcggcgtgc cgtggctgtc gctgccggac
      121 accatcagca cgtcattccc cttcaggcgg tggtagcggg cgaaaatgtc gggcggcagg
      181 tacgcgcccg cgatatggcc gagatgctgc gggccattgg cgtacggcca ggcgacgcag
      241 acgaggattt tatcgggcat caggttccct agaacggtga tacaccgatt ttagcatact
      301 tgaagcgaat cgcgtatcgc ctatagctga tggctgattg cctttgccca ccaatcaaac
      361 cgccagactc atgctggcgg ttgagacact atgtccacgt ggccgaccca gttggtccgt
      421 gctttaccga cgacgtctgc cgctttgccg ggcgcgctga cccactcacg ggttctgcag
      481 caagccgata gctgcgttca ggcgatcgcc ggcctcatta acgcctatgc gcgcgacatc
      541 aaaggccagg ttgccgatcg tgcccccacc tgcttcgcac acctcacgaa tcgagttgac
      601 cttatcggtg atgatggcga cggcctcgcg gtacaggcta taggcactct gcacctggga
      661 cggctgctgg ttcacatcgt gtgtcgccgc gttcacgatg gcgtagaaat cggcgaggaa
      721 tggcgcgcag gcttcagccc cgctgccggc atagatgcga tccatcgagc cgcccagcgc
      781 gtgcacgcga ttcaacatat ctcgtgcagc gctgagcaag ggcggtacgg gtggctgggt
      841 cggcgtggcc gtcgcccgcg gcgtgttcgt cgctcgtggc gtaggcgtgc ggctgggcgt
      901 tgatgtcgat gtgggcgtat cggtcggtgc ggccgttggc gtactcgtcg gctcaggtga
      961 atccgtcggg gtgctggtcg gctcgggcgt gttggtgtga gtgggtggcg gcgtctcgct
     1021 agggggtggc tccgtaggta cgggcgcagg agtgggcgtg gcgcacgcag ctaccagcgc
     1081 cacactcagc ccagccacga taatgaggcg ccaacgggcg gacagtatct tcatggacaa
     1141 ctccttccga cacgtacccc ccacctgctc gcattatact acgaaaccgg agcagtgcgc
     1201 tgccggacgt catcagcacg gtgtctccac taccggcatt cagttcgccc tgttagcgcc
     1261 ttcacactgg attcgaaaaa caaccacgct ttctcgagat aagtggcggc cagatctccc
     1321 ccattttctg agcaccattg ccacgctagc gtgccgccag caggcgcgca gagggaggcg
     1381 ggcaccctgc aggccgtgca gcatcgactc cagccgaacg ggcgtagcgg tgtgcgaggg
     1441 taagggcacg ggcagatgga gccgggtgaa gcgagatatt cagattcact cccctcgaat
     1501 catcctcttg ctgatccgat tgtgcttctc aatcaccaat ctccaatcaa ccgtctgtaa
     1561 ttcgccgtcc tcgaccacga cccgcccatt gatcaccgac aaatcgacgc gctgcggcgc
     1621 gcagaacatc agcgcggtga gcggatcatg cagcgcgccg gcgtagtcga gccgctccag
     1681 cttgaagccg atgaagtcag ccgccttgcc gggcgcaagc tcgccgacat cgtcacggcc
     1741 caacaccgcc gccccgcccc gcgtcgccag ccacaaggct tcgtaagctt tcaagcctcg
     1801 cggattaccc gtcacgcgtt ggagcaggag tgcctgccgt gcctcggcca gcatgtgcga
     1861 tccgtcgttg ctggcggaac cgtccacgcc aagcccgacc ttcaccccga gatcgaccat
     1921 gcgccgtacc ggcgctatgc cgctcgccag ccgcatgttg ctcgtcgggc agtgcgccgc
     1981 gccggtgccg ccgccggcca tgatgccaat ctctttttcg tcgacgtgaa cactatgcgc
     2041 ccaccacaca tcgtcgccta cccagccgag ttcggcggcg tacatggccg gtcgatgccc
     2101 gaatttctcc aggcagaatg cttcttcgtc tttcgtttcg gccagatgcg tatgcaaggt
     2161 gacaccatac tggcgcgcga ggttggccga ctcgcgcatc aggtcgggcg tgaccgagaa
     2221 gggtgagcac ggcgcgacga cgacgcgcag catcgcgtac ggcttcgggt cgtgatatcg
     2281 ctcgatgacc cgcacacaat ccctaattac caattcctca tcttccacga cacgatccgg
     2341 tggaagccct cccttgctct cccccaacga catcgatccc cgcgcggcat ggaagcgcag
     2401 gccgatctcc tgcgcggcgc ggatctcgtc gtcgagcctc gcgccgttgg ggaacaggta
     2461 gagatgatcg ctggatgtcg tgcacccgct cataatcagc tcggccatgc ctgtgagcgc
     2521 gctgatgtac accgcctcgc cgtccatgcg cgcccaaacg ggatacagcg tcttgagcca
     2581 gtcgaacaag cccgagtcgg gcgccagcgc gcgggtgagc gtctggtaca ggtgatggtg
     2641 cgtgttgacg agcccaggaa gaacgaccat gccggacgcg tcgacgacgc ggtcggcggc
     2701 gagatatgcg ggaggcaggt cgcgcgccgg gccgacccag tcaatcacgt tgtcgcggcc
     2761 gtagagcgca ccgtccggga tcgttcgtcc cgcatcgtcc atcgtaatga gtaattgggc
     2821 attgcgaacc agaaatgttg acacaattca cctcgccgtt aaacacgaag acacaaggaa
     2881 ttgtcattct gagccttgcc ctgagcggag tcgaaggcgc agcgaagagc ctcgcaaagg
     2941 aatgttcaac ctcgtgtcgc tgcgtgtgga ttcgtggaat gcctccacgg taaagtgccc
     3001 cgattgtacg ggcgggaggc aggcaagtca attcgcttat tgcgcatcgc ctatcgcgtg
     3061 tcgcacagcg cttatcgctt cgctccttcc tgcgtcggct cgcgcagggc gagcggctgg
     3121 ttctggcttt ctactcgctc gcttttctgc ttcggaccag caagtagatc gcgacgccgt
     3181 aggcaacaac tgatagtcgc gtcggacgac tgaaatacga cttcacgttg atccgaacag
     3241 cgccctgaga tagagcctgg cgctgaatgg ctgcccggcg agcagcgtct gagcatgaaa
     3301 catcctggca accagacgta cgatgaagac cgctgtgacc gccatcaagg ctgctgccaa
     3361 ccagggctgc caccacggca caccgccgac gaccagccgc gccaacatcg tgaagggcgc
     3421 agtcagcggg aacaaactca ggccggtggc cagcgcgccg tgcggttcga tgggcagcat
     3481 ggggctgaac acaaacgaga ctaacatcgg ccagatgatg agaaggatga actgggacgc
     3541 ctcgcgccgg ctggaagcca acgcgcccag tccggccatc aggctgccgt acacggcgta
     3601 gctcgacacg aagaaggcga tcccccaaaa tacgatcgac ggcgacaatt cgaggccggg
     3661 cggcagcgcg acgcggccgc cggcgcccag caggaagtag gccgtaccga accagacggc
     3721 cgcctgcagt agacccagga tgcccagcgc ggcgaccttg ccaatcagca tttgccgcga
     3781 agtaatcgac gatagcagga tctccatcgt ccggttctgc ttctccacgc tcacgctgtt
     3841 gagcagcaag ccggcggaca tcatcagtac catgccggag agcatcatca tgccgtaagg
     3901 gatgtagaaa accagtggtg aatccacctc ctgccgcatg tccgcaggcg ccagcgccgt
     3961 gacgtgcacg ttcatcgcat tcccggcgcg catccgttcc gggtcgttgc ccaccaggtt
     4021 ggcgaatagt gtccagcgca tcacccatgc ctggccttca gcggacacgg gccgataatt
     4081 cggattgacg tagatcaggt tccccgtttc gagatagtcc ggcggcacga ggtagtaggc
     4141 ggcgatctct cctgactgca tcgcccgccg ggcgctgtct tcgtcaggat aggccgtcag
     4201 gattccccga ggaacgtcgg gatggatctc ctcgatcaag ccgctcaaat cgacgtaccc
     4261 ttcgcgctgg agtttccgca tgtcaggccc actcacagag ccggtcggcc cgctgggttg
     4321 gctttctccg atccgggaca agaccacgaa cgccaggaag aggattacag gcaacccgaa
     4381 aacggcgaac aggaacgatc gccttctgaa tgctgtagag atctcatagc gtaaaaccag
     4441 cagagtcttg gacatgaggc tttctccagg aaaactccgt ggccgacgca tccctatgcg
     4501 tcggcgggca tcgagatgcc taccctacgt tttcggatag gcgcttagcg gcgtggcatg
     4561 tcgcttgccg gcggcgaaca gttcgcgcca gcgcagacgc tgcccgtagc gcagcatccc
     4621 caggcgaaag gcgcgcccgg ccagccacac agcgccggct gcacacacga cctgcacaag
     4681 cgcgctcgcc gcgatctgcc aaaatggcac cggcgtgaag acgatgcgca ggctgagcgt
     4741 cgacagggcc gtgggcggga agaagctcaa accggtcgcc agtgggccgt tcgcatcctg
     4801 aatgatgatg atggccagcc agtacggcgc ccacatgggc accgcaaaca ggccggtcaa
     4861 ctgttgggcc tcgtgcgcct cggcgacggt cgcgcccaga gcaatcatca gcgccgcgaa
     4921 ggtcacgaat gtcggaacgc cgacgatcgc gagttgggcg atgagttgcg gatcgagcga
     4981 caatccttgg agtacactga tgcctaactg gcgttcaccc actccgatgg ctaggccgat
     5041 gaggatgttc caggcgacaa cctgcgtcaa ggcgatggcg ctgatgccga gcaccttgcc
     5101 agccattaac tgattgggcg agatggacgt caccacgatc tccatggtgc gattctcttt
     5161 ctcctcggcc accgcgctca tcaagtaacc cgagagcatg agcagcagca tgatgaaggc
     5221 ggcgcccgcg aacgccggga tgaagttgcc gaggatcatc cggtcgtcga actcgcgccc
     5281 gccgcccggc atgtcctccg gccagcgcac gatcaggtag ctgccttcga ctgcgcgccg
     5341 ggcaacttcc ggcggcaggc cggccagccg gttgatctgc atgaaatccc agaactgatc
     5401 cgtagcgttt tcgccgggcg gctcgacgta caccagctcg acgcggttgg tcgcgaagta
     5461 attctcggtc acgacgtagt aagcctggat gtctccggac aggagcgcag catgggccgc
     5521 atcctcagtc tcgaacggga tcagcgctac cggctcgggg ctgcccgggc cggatgccga
     5581 tccctccgcc tggggcacgg ggatgggatc ggccagcagc ccggcgtggt cgacatagcc
     5641 gaccggggcg ctgttgttct ccatgctttc gatcagccag cctagcccga tcatcgacgc
     5701 gagcatcagg ggcacgctca acacgacgag cacgaagctc cttcgaaaga cgagccggcg
     5761 gtattcgtag gtcgcgacgc cccacatctt acgcatcagg cacctcctcc gataccgctc
     5821 ggatgaatat ctcattcaac gagggtacgg cgatctcgaa cttctccaat acgacaccgc
     5881 gctcgaccag cgccttcagc acgtcctggg gagtgctgtc attgccgaga gttagtttgg
     5941 tgacgtgatt gaccaccgtc atcgcttcga cgcccgtcag gtgcggcagt tcacccacgg
     6001 cgctgaccag cacagcatcg cccacgtatc ggcgacggat ctcttccagg ttgccataca
     6061 acaggttccg gccctggttg atgagcagga tacggtcgca caac
//
LOCUS       DBNHXB010000146         6291 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4058315, whole genome shotgun sequence.
ACCESSION   DBNHXB010000146 DBNHXB010000000
VERSION     DBNHXB010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4058315"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="AC162G_05655"
     CDS             complement(<1..720)
                     /locus_tag="AC162G_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5075331.1"
                     /translation="MEFKTLLYELEEGIATITLNRPDVLNAISMDMRADFTALAETLF
                     FDDAVRVVVFTGAGRAFSAGGDISHFEQDWLTPKFRANSRRLIKFFDDLEALEKPVIA
                     AMNGAATGGGLELALACDIRIASEEATLGFRENAIGLIPGIGGCARFVRLIGVARAKE
                     LIFTGKMISATEAEAMGLVNRVVPPDRLMDETRELTRMLLKRAPQSIGLAKKVLNTVA
                     HLDQTSGIMMEGLAQSILLKTE"
     gene            complement(756..938)
                     /locus_tag="AC162G_05660"
     CDS             complement(756..938)
                     /locus_tag="AC162G_05660"
                     /inference="COORDINATES: protein motif:HMM:NF049491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaD-like zinc ribbon domain-containing protein"
                     /protein_id="HIR5075332.1"
                     /translation="MELELEYPQSETVTCAFCNSTDTEMISLFGQFLLASQYYCRNCH
                     SVFEAVRWSEETPGES"
     gene            complement(941..1705)
                     /locus_tag="AC162G_05665"
     CDS             complement(941..1705)
                     /locus_tag="AC162G_05665"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0062077 - phenylacetyl-CoA 1,2-epoxidase
                     complex [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HIR5075333.1"
                     /translation="MSATTYAPRYQSAANLSPAVNAALLQFGLALADTKHRYGVRLSE
                     WSHSAPALEASVGCASMTQDELGHARSLFAMLRDLPGAPADLNPETDMTREKHYNPRF
                     LDAPWPSWADVIAANVLLDRALNVVFESARRSEYGPLGGRVAKILQEEHFHHVFGESW
                     LARLASGSEAAHDRLQAALNRVAPVASAWFGPPGDATLAALVDAGILSANSSQLREAW
                     LSRVRPLLDRCGLSLPAVEHDWSTWNAERREAESRS"
     gene            complement(1702..1908)
                     /locus_tag="AC162G_05670"
     CDS             complement(1702..1908)
                     /locus_tag="AC162G_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075334.1"
                     /translation="MNQTPQTYHLYGRKSYPQPLTHIGQLEADSQANLKDAALRLAGA
                     NGWVELVAIPASSITPVIVAGEPV"
     gene            complement(2061..2276)
                     /locus_tag="AC162G_05675"
     CDS             complement(2061..2276)
                     /locus_tag="AC162G_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075335.1"
                     /translation="MSQPPAHTPEGDVYEVFAKFQADEPLHHVGSVIAANRELARAYA
                     FTLYNEWAWNEMIVAPRQDVIVLVEAV"
     gene            complement(2316..2681)
                     /locus_tag="AC162G_05680"
     CDS             complement(2316..2681)
                     /locus_tag="AC162G_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIR5075336.1"
                     /translation="MTLASQVAGRTQQARLNATPDEVGLWQALEDVQDPEFPMSVVDM
                     GLIYGLRRHANRVQVYITFTAMGCPCMEYIIGDVRERLLREPGIDDVEIQIVWDPPWT
                     RQRLSAKGIEKLKRWGVSA"
     gene            complement(2723..3658)
                     /locus_tag="AC162G_05685"
     CDS             complement(2723..3658)
                     /locus_tag="AC162G_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HIR5075337.1"
                     /translation="MTLVAETELTDRLHAGKLVETIDQMSPIYLEGIKRILTVSGDTE
                     LISAPAYRLAANDAPSTNAFVSAMGIIQDEMAHAHIAYRLLEDLGVDKEWLVYERKPG
                     EFKYPYAFDVPLETWVELVVANALYDRAGYVLLGDVFKHGSFGPWKRALVKVDKEENF
                     HLRHGETWMRKINEQPGGHAKLQRAVDWMLPLTLEWFGLPNNLKRHTEQIEYGFKGST
                     NDELRQTWMSTAIPFLQELRLDVPVHYDSTAGRYVIDFPFPCRFVPDEKRWLFEEGPC
                     TWDDVLRRWKARGPKNQEFIEQIQRGRNELNALVM"
     gene            complement(3719..4129)
                     /locus_tag="AC162G_05690"
     CDS             complement(3719..4129)
                     /locus_tag="AC162G_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015201653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5075338.1"
                     /translation="MPAKDHRELRVYQLAFETAVRIQQLTKAFPQEERYSLTDQIRRS
                     SRSVCANIAEAWRKRRYPKNFVSKLSDADSEAAETQVWLDFALHFSYFSADIYEELVD
                     RYNHICSQLSLMMDEPEKWMPRRAATTQNRKRNS"
     gene            complement(4203..4985)
                     /locus_tag="AC162G_05695"
     CDS             complement(4203..4985)
                     /locus_tag="AC162G_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5075339.1"
                     /translation="MSDPVVTFSKSDAVGFITLNRPPANSYDISFMRDLDAAIEAANG
                     DAGVKVVILKSASEKFFSAGADVKAFMANTTAANMEMIRFAHQALDKMAASAKVFIAA
                     INGHALGGGLEMALACDLRFAAEGSYRIGLPEVTLGLLPGNGGTQRLPRLIGANKALE
                     MMITGEAVAPADALRLGIVNRLFTPDKLMEETEAYARKLASGASVAVGRIKHAVYRGI
                     ALPLSEGLALERELIEPLFNTEDAKEGFAAFAEKRQPVYKGK"
     gene            complement(5133..>6291)
                     /locus_tag="AC162G_05700"
     CDS             complement(5133..>6291)
                     /locus_tag="AC162G_05700"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5075340.1"
                     /translation="LHGMEFYAGLASKIRGAQVPLPPALGKHAYGLVLKRPVGVCVGI
                     VPWNFPLTLMGTKVGPALIAGNPIIIKPARTTPLATLKIIGLLNEAGLPPGVLQCVTG
                     VGSAVGETLICHPAVRRVALTGSSETGRRVMELAGPDFKRITLELGGSDPCIVCPDAN
                     VKRAVSGIVVGRYWNAGQQCLAVKRLYLFEEIYDQFMEGLLEKVGNYRVGDGSTRPDK
                     PFIRMGPLHTAYQRQEIEDQLADAASKGAKVLIGGKRPDDPALAQGHFFEPAVVVDAP
                     QDGKLATEEVFGPVLPVWKVKSLDEAIQKANASQWGLGSSIWTQNMSWANRAAREIQA
                     GVTWVNQIHYGYDELPFGGVKASGVGHEHGPEAVDYYLENKGVVFGGLDWE"
BASE COUNT         1117 a         2029 c         1873 g         1272 t
ORIGIN      
        1 ttcggtcttc aaaaggatgc tctgcgccag gccttccatc atgatgccgg acgtctgatc
       61 gagatgcgcc accgtgttga ggactttctt cgccaggccg atcgactgcg gcgcgcgctt
      121 gagcagcatg cgggtcagct cgcgtgtctc gtccattagg cgatccggcg gcacgacgcg
      181 gttgaccaac cccatcgctt cggcctccgt ggccgagatc atcttgccag tgaagatcag
      241 ctccttcgcg cgcgccacgc cgatcagccg cacgaagcgg gcgcagccgc cgatgcctgg
      301 gatcagaccg atggcgttct cgcggaagcc gagggtcgcc tcttcggacg cgatgcgaat
      361 gtcgcaggcg agcgccaact ccagcccccc acccgtcgcc gcgccattca tcgcggcgat
      421 gaccggcttc tccagcgctt cgagatcgtc gaagaacttg atcaaccggc ggctgttggc
      481 gcggaacttc ggtgtcagcc agtcctgttc gaagtgactg atgtcaccgc cggccgaaaa
      541 cgcgcggccc gccccggtga agacgaccac gcgcaccgca tcgtcgaaga acagcgtctc
      601 cgccagcgcc gtgaagtcgg cgcgcatgtc catgctgatc gcgttgagca cgtccggccg
      661 gttcagcgtg atcgtggcaa taccctcttc cagctcataa agcagcgtct tgaattccat
      721 cagactcctc taacccatga ctctaactca tgactctaac tctcaccggg cgtctcttcg
      781 ctccagcgca cggcttcgaa cacggaatgg caattgcggc agtaatactg cgaggcgagc
      841 aagaactggc cgaacagcga gatcatttcc gtgtcggtcg agttgcagaa ggcgcacgtc
      901 accgtttcgc tctgtgggta ctctaactct aactccaact ctaactccta ctctccgcct
      961 cccggcgctc cgcattccag gtcgaccagt cgtgttcgac ggcgggtagc gacaggccgc
     1021 atctgtccag cagcggtctg acccggctca gccaggcttc gcggagctgg gacgagtttg
     1081 cagagagaat gccagcgtcg acgagcgcag ccagcgtcgc atcgccgggt gggccgaacc
     1141 acgccgatgc gaccggcgcg acccggttca gcgccgcctg caatcgatcg tgcgctgcct
     1201 cgctcccaga ggcgagacgc gccagccagc tttcgccgaa cacgtgatga aaatgctctt
     1261 cctggaggat tttagcgact cgcccgccca acggtccgta ttccgagcga cgcgccgatt
     1321 cgaacacgac gttcaacgcg cgatcgagca ggacattcgc ggcgatcacg tccgcccacg
     1381 acggccacgg cgcgtcgagg aagcgaggat tgtaatgctt ctcgcgggtc atgtcggtct
     1441 cggggttgag atcggccggc gcgccgggca gatcgcgcag catggcaaac aggctgcgcg
     1501 cgtggcccag ctcgtcctgc gtcatcgacg cgcatccaac cgaggcttcc agcgccggtg
     1561 cgctgtgtga ccactcggac aagcgcacgc cgtagcggtg cttcgtatcg gccagcgcca
     1621 ggccgaattg cagcagagcg gcgttgacgg ccgggctgag gttggctgcc gactggtaac
     1681 gaggcgcata cgtcgtcgcg ctcataccgg ctctcccgcg acgatgaccg gcgtgatcga
     1741 cgacgccggg atcgccacca gctcgaccca gccgttcgct ccggccaggc gcagcgctgc
     1801 gtctttcagg ttcgcctggc tgtcagcttc cagttggccg atgtgcgtta gaggctgagg
     1861 gtaagatttg cgaccgtaga ggtgataggt ttgaggtgtc tggttcatgg ttattggtaa
     1921 ttggtaattg tggagtgtgg ggcgagtgtt ctgtcataaa tcaccccatc aatgtgttct
     1981 tcgcttcacg cctcacgctt cacgtttcac gcttcacgat ttacgcttta cgtttcacgc
     2041 ttcacgcaac accttccccc tcacactgcc tctaccaata cgatcacatc ctgcctcggc
     2101 gccacgatca tctcgttcca cgcccactcg ttgtacagtg tgaacgcgta cgcgcgggcc
     2161 agctcgcgat tggcggcaat cacgctgccc acgtggtgca gcggctcgtc tgcctgaaac
     2221 ttggcgaaca cctcataaac gtcgccctcc ggcgtgtgtg caggtggttg gctcatacgc
     2281 ttaccttcta gggcgcatgc aagacgcgcc cctgctcatg cactcactcc ccatcgcttg
     2341 agtttctcga tgcccttcgc cgacaggcgc tggcgcgtcc agggcggatc ccacacgatc
     2401 tggatctcca cgtcgtcgat ccccggctcc ctgagcagcc gctcccgaac gtcgccgatg
     2461 atgtactcca tgcaagggca gcccatagcg gtgaacgtga tgtacacctg cacacggtta
     2521 gcatgccgcc gcagaccgta gatcagcccc atgtccacca cgctcatcgg gaactcggga
     2581 tcttgaacgt cttcgagcgc ctgccacaat ccgacttcat caggcgtggc attcaagcgg
     2641 gcctgttgag tgcgcccggc gacttgcgac gcgagggtca tttcaatctc caatctctaa
     2701 tctcggcatg aggagacgcc tcctacatga ccagggcgtt caattcattg cggccgcgct
     2761 ggatctgctc gatgaactcc tggttcttcg gtccgcgcgc cttccaccgc ctgagcacgt
     2821 cgtcccacgt gcaagggcct tcctcgaata gccagcgctt ctcgtccggc acgaagcggc
     2881 aagggaaggg gaaatcgatg acgtacctac ccgccgttga gtcatagtgc acaggcacgt
     2941 cgaggcgaag ctcttgcagg aagggaatgg cggtgctcat ccaggtctgg cgcagctcgt
     3001 cgttggtgct gcctttgaag ccgtattcga tctgctcggt gtggcgcttc aggttgttgg
     3061 gcagcccgaa ccattcgagc gtgagcggca gcatccagtc gacggcgcgc tgcagctttg
     3121 cgtggccgcc gggctgctcg ttgatcttgc gcatccaggt ctcaccgtgt cgcaggtgga
     3181 aattctcttc tttatccacc ttcaccagcg cgcgcttcca cggcccgaag ctgccgtgct
     3241 tgaacacgtc gcccaggagc acgtagccgg cccggtcgta cagcgcgttg gcgacgacca
     3301 gctcgaccca cgtttcgagc ggcacgtcga aggcgtaggg atacttgaac tcccctggct
     3361 tgcgctcgta caccagccat tccttgtcta cgccgaggtc ttccagcagg cgataggcga
     3421 tgtgcgcgtg cgccatttcg tcctggatga tgcccatcgc cgagacgaag gcgttcgtcg
     3481 acggcgcgtc gttcgcggcg aggcggtagg ccggcgcgct gatcagctcg gtgtcgcccg
     3541 acacggtgag gatgcgcttg atgccttcca ggtagatcgg gctcatctgg tcaatcgtct
     3601 cgacgagttt gcctgcgtgg agacgatctg tgagttccgt ttcagcgact agtgtcatat
     3661 gtgtactcct tcttcgatcg agcgtggagc gtggagcgtg aagcgtggag tgtgacagtc
     3721 atgaattgcg ctttctgttc tgagttgttg cagctcttcg aggcatccac ttttcaggct
     3781 catccatcat gagcgaaagc tggctgcaga tatgattgta acgatcgacc aactcttcgt
     3841 aaatgtcggc ggagaaataa ctgaagtgca gtgcgaaatc cagccagacc tgcgtctcgg
     3901 ccgcctcgct gtcggcgtcg ctcagtttgc tgacgaaatt cttggggtag cgtcgcttac
     3961 gccacgcctc ggcaatgttg gcacataccg aacgagatga gcggcgaatc tgatcggtca
     4021 ggctgtagcg ctcctcctga ggaaaggctt tcgtgagctg ctgaatgcgg actgccgttt
     4081 cgaatgccag ttgataaacg cgtagctcac ggtggtcttt ggcgggcatg gtatcctcct
     4141 ttgtggagcg tggagcgtcg agcgtcgagc gattcgcttc acgctccaca ctcgacgctc
     4201 cactactttc ccttgtacac gggttgtcgc ttctccgcaa aagccgcgaa tccttctttg
     4261 gcatcttcgg tattaaacag cggctcgatc agctcgcgtt ccaatgccag cccctcgctc
     4321 aacggtagcg cgatgcctcg atacacggcg tgcttgatgc gacccaccgc gaccgatgcg
     4381 ccggacgcca atttccgggc gtaggcctcc gtttcttcca tgagtttatc gggagtgaac
     4441 aggcggttga cgatacccaa ccgcagcgcg tcggcgggag cgacggcctc gccggtgatc
     4501 atcatctcga gcgccttgtt ggcgccgatc aggcgcggca ggcgctgcgt gccgccgttg
     4561 ccgggcagca atccgagcgt gacttccggt aacccgatcc ggtagctgcc ctcggcggcg
     4621 aagcgcaaat cacaggcgag cgccatctcc agcccgccac cgagcgcgtg gccgttgatc
     4681 gcggcgatga acaccttggc gctggcggcc atcttgtcga gcgcctgatg ggcgaagcga
     4741 atcatctcca tattggccgc cgtcgtattc gccatgaagg ctttgacgtc ggcgccggcc
     4801 gagaagaact tctccgaggc gctcttgagg atgacgacct tcacgccggc atcgccgtta
     4861 gccgcctcga tcgccgcgtc gaggtcgcgc ataaaactga tgtcgtagct gttcgccggt
     4921 gggcggttca gcgtaatgaa gcccacggca tccgacttcg agaaagtgac gacagggtca
     4981 gacatgattc ctccttggat ggctgattta gaatcagtta tcagtcatca gttatcagtg
     5041 cccactgctc accgattact gttcactgat caccgatcac tggtcactgg tcactggtca
     5101 ctggtcactg atcactgatc actgattacc gatcactccc aatccaaacc accaaacacg
     5161 acgcccttgt tctcaaggta gtaatccacc gcttccggcc cgtgctcgtg accgacgccg
     5221 gacgccttga cgccgccgaa cggcaattcg tcgtaaccgt agtgaatctg gttcacccac
     5281 gtcacgccgg cctgaatctc gcgcgcggcg cggttcgccc agctcatgtt ctgcgtccag
     5341 atcgaagagc cgagacccca ttggctggcg ttggctttct ggatcgcctc gtcgagcgac
     5401 ttgactttcc acaccggcag gacaggcccg aagacttcct cggtcgccag cttgccgtcc
     5461 tgcggcgcgt cgacgacgac ggcaggctcg aagaagtggc cctgcgccag cgccgggtcg
     5521 tcgggccgct tcccgccgat gagtaccttc gcgcctttcg aggcagcatc cgccaactgg
     5581 tcttcgatct cctggcgctg atacgccgtg tgcagcgggc ccatgcggat gaacggcttg
     5641 tcgggcctgg tgctgccgtc gccgacgcga tagttgccga ccttctccag gaggccctcc
     5701 atgaactgat cataaatctc ttcgaagaga tacaaccgct tgacggcgag acactgctgc
     5761 cccgcgttcc agtaacgccc gacgacgatg ccgctcacgg cgcgcttgac gttggcatcg
     5821 ggacagacaa tacacggatc ggagccgccc agctcgagcg tgatgcgctt gaagtcggga
     5881 ccggccagct ccatcacgcg ccggcccgtc tcggacgatc cggtcaacgc gacgcgccgc
     5941 acggccgggt ggcagatcaa cgtctcgccg acggcgctgc ccacgccggt cacgcactgc
     6001 agcacaccgg gcggcagtcc tgcctcgttg agcaggccga tgatcttgag cgtggcaagc
     6061 ggcgtggtgc gggcaggctt gatgatgatc ggattgcccg cgatcagcgc cggcccgacc
     6121 ttggtgccca tcaacgtgag cgggaagttc cacggcacga tgccgacgca cacgccgacc
     6181 ggccgtttga gcaccaggcc gtaagcgtgc ttgcccagcg cgggcggcag cgggacctgc
     6241 gcgcctcgga tcttgctggc gagtcccgcg tagaactcca tgccgtgcag a
//
LOCUS       DBNHXB010000147         4097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1038684, whole genome shotgun sequence.
ACCESSION   DBNHXB010000147 DBNHXB010000000
VERSION     DBNHXB010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1038684"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..388
                     /locus_tag="AC162G_05705"
     CDS             38..388
                     /locus_tag="AC162G_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001540 - amyloid-beta binding [Evidence
                     IEA];
                     GO_function: GO:0004930 - G protein-coupled receptor
                     activity [Evidence IEA];
                     GO_process: GO:0097190 - apoptotic signaling pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HIR5075341.1"
                     /translation="MANVVQYLPELDPDEQAALQPMMSDMDEAKAQRFAFAYRSRRRD
                     PQVILLTTLLGFFMIAGVQRFLLGQIGMGLLYLFTGGLCLIGTIIDLVNYKKLALEYN
                     LKEAQQLAAMIKAA"
     gene            436..720
                     /locus_tag="AC162G_05710"
     CDS             436..720
                     /locus_tag="AC162G_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HIR5075342.1"
                     /translation="MKVNFYATYRQIVGGKTVDIPTAKSVTVRQLIDEVIKCYPRLRA
                     ELLDDGGQLYGHVHVFISGRDAPYLVEGLDTTLTEDDTVNIFPPVAGGAH"
     gene            complement(749..2005)
                     /locus_tag="AC162G_05715"
     CDS             complement(749..2005)
                     /locus_tag="AC162G_05715"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5075343.1"
                     /translation="MTNTESTQWAEVDEQGRLVLPAEVAARYGLKPGARARIEPDTNH
                     LRLHRPVTHLAKVYVEPTSLCNIACRTCIRNVWDEPMGMMSAATFERIVEDLTALTPA
                     PTVFFGGLGEPLFHPRVIEMVTRVKAIGASAELITNGTLLDEERARSLIESGLDVLWV
                     SIDGATPESYADVRLGATLPEVLDNLARFRQLRPGSHRPTPHIGIAFVALKHNIAELP
                     EVIALGRRLGASRFMVSNVLPYTPEMYQEILYGRALTDIAYIPSPWLPHLSLPKMEMS
                     EFTQEAFRGALRSGCNVTFAGNNLGGANDACAFVEGGSTSIGWDGSVSPCLALLHSHV
                     TYLNGRERRMRRHVMGNVAERRLLDVWSDPSYVAYRQRVQGFAFAPCTFCGGCELIEG
                     NEEDCLGNTFPACGGCLWAQGIIQCP"
     gene            complement(2253..4073)
                     /locus_tag="AC162G_05720"
     CDS             complement(2253..4073)
                     /locus_tag="AC162G_05720"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR5075344.1"
                     /translation="MTELGGYANKIGRIDLTTRNVTYEEIPEEWARKYIGGRGLGDRY
                     VFENGPQVDPLSPDNILCFMNGPLTGSEANMSGRMAVVTKSPLTGTITDSHHGGWSAA
                     RLRWAGFDGLIFKGKADKPVYAYVHDGAVEILDASDVWGKGVHDTVKFFQEKYGEKDL
                     TVIAIGPGGERLARFANWMNENDRSSGRGGTGCVGGSKNLKAIVVKALKKMHKAKDRA
                     AWKPAHDRALATIMDEKNVTSPRKGGLSVYGTNVLTNIANNMGALPTRNSQLTSFGEK
                     AERTGGEWVKEHILVDDPTCHACPVACKKEVEIEEGPFAGLRMESQEYESVWAFGANC
                     DNDDSAGIAKLINVCNDYGLDTIEMGNVLSMYMEASQRGYTNGTGGLRWGDVMGMVET
                     VRKIAFREGIGDVLAEGTARAAQHFGHPEISMTVKGQAIPAYDPRGMKGMGIGYATSN
                     RGACHLRGYTPASELGLIPLKTDPLAWKGKGELVKTFQDLFAFTDSLDLCKFSTFAEG
                     AEEYAAQYTAMTGLPFTADDVMKTGERIYNLERYYNNLAGFREGSDYLPERFLKEPST
                     MPGSQGHICELPEMLDEYYQVRGWKDGVVPETKLKELGVL"
BASE COUNT          693 a         1312 c         1263 g          829 t
ORIGIN      
        1 tcaaccttcg tagtaacctt cggcaaagga gatcggcatg gcaaacgtgg tgcagtatct
       61 acccgagctt gatccggatg agcaggctgc tcttcagccg atgatgagcg acatggacga
      121 ggcaaaagcg cagcgctttg cgttcgcgta ccgctcgcgc cgaagggatc cgcaggtcat
      181 cctgttgacg accttgttag ggttcttcat gattgcgggc gtccagcgct ttctgctcgg
      241 ccaaatcgga atgggactct tgtatctgtt caccggcggc ttgtgcctca tcggcaccat
      301 tatcgatctc gtcaactaca agaaactcgc cctggagtac aatcttaagg aagcacagca
      361 gctcgcggcg atgattaaag ccgcgtaact gcgaggcggg gctggcctct cgtgcacatc
      421 gagcgggagg tagcgatgaa ggtcaatttc tacgccacct atcggcagat cgtcggcggc
      481 aagaccgtcg atattcccac tgcgaaaagc gtcaccgtcc ggcagttgat cgacgaagtg
      541 atcaaatgct accctcgact gcgggccgag ctgctcgacg acggcggcca gttgtatggg
      601 cacgtccacg tgttcatcag tgggcgcgac gcgccctacc tggtcgaggg gcttgacacg
      661 acactgaccg aagacgatac ggtgaacata tttccgcccg tcgctggagg agcgcattga
      721 agcaggtgga agataacctg aaattcccct acgggcactg aatgattcct tgcgcccaca
      781 ggcagccgcc gcaggccggg aaggtgttgc ccagacagtc ttcttcgttc ccctcgatca
      841 actcgcaccc cccgcagaac gtgcatggcg cgaaggcgaa accctgcacg cgctggcggt
      901 acgcgacgta gctcggatcg ctccacacgt cgagtagacg ccgctcggcc acgttaccca
      961 tcacgtggcg gcgcatacgg cgctcgcgac cattcagata ggtgacgtgg ctgtgcagca
     1021 gcgccaggca cgggctgacg ctgccgtccc acccgatcga cgtcgagccg ccttcgacga
     1081 acgcgcacgc atcgttcgcg ccgccgaggt tattcccggc gaatgtgacg ttacagccac
     1141 tgcgcagtgc gcctcgaaac gcctcttgcg tgaactcgct catctccatc ttgggcaggc
     1201 tcaagtgcgg cagccacggc gacggtatgt aggcgatgtc cgtcagcgcg cggccgtaca
     1261 ggatttcctg atacatctcc ggcgtgtagg gcagcacgtt gctgaccatg aagcgcgacg
     1321 cgcccaggcg gcggcccagt gcgatgacct ctggcagctc ggcgatgttg tgcttcaggg
     1381 ctacgaaggc gatcccgata tgcggcgttg ggcggtgcga tcctgggcgc aactgcctga
     1441 agcgggctaa gttgtcgagc acctcgggca gggtcgcccc cagccgcacg tcggcgtagc
     1501 tctccggcgt ggccccgtcg atggataccc acagcacgtc gagaccgctt tcgataagag
     1561 agcgcgctcg ctcctcgtcg agcagcgtcc cgttcgtgat cagctctgcc gaagccccga
     1621 tggccttgac gcgggtgacc atctcgatga cacgtggatg aaacagcggc tcgcccaggc
     1681 cgccgaagaa gacggtgggc gcgggcgtca aggcggtcaa atcttcgacg atgcgctcga
     1741 acgtcgccgc gctcatcatg cccatcggct cgtcccagac gttccgtatg cacgtgcggc
     1801 acgcgatatt gcacaggctg gtcggttcga cgtacacctt agccaggtgc gttaccggcc
     1861 ggtgcaggcg aagatgattc gtatccggct cgatgcgggc gcgcgcgccc ggcttcagtc
     1921 cgtagcgggc ggcaacctct gcgggcagca ccaggcggcc ctgctcgtcg acctcggccc
     1981 actgtgtgga ttcagtattt gtcatggggt aatacgatag acctggcagg tctcatccgc
     2041 cgctggtggc taagcgccag gccgcgaaga cctgccaggt ctggtcggaa agtccgagta
     2101 ggtgtggttc aaagatctcg atttgagcca cggccgagac ttcggaagtc tttaagtggc
     2161 acctcgccag cgacgctact cggatttccg gccaccgtcg cctgcgagaa cacgccttga
     2221 gacttccgaa gtctgagaat cgtcgactga gactacagaa cgcccaactc cttcagtttc
     2281 gtttccggca ccacgccatc cttccagccg cgcacctggt agtactcgtc cagcatctcg
     2341 ggcagctcgc agatgtggcc ctgagagccg ggcatcgtcg acggctcctt caggaagcgc
     2401 tccggcaggt agtcgcttcc ttcgcggaag ccggccaggt tgttgtagta gcgctccagg
     2461 ttgtagatgc gctcgccggt cttcatcacg tcgtcggcgg tgaagggcag tccggtcatc
     2521 gccgtgtact gggcggcgta ttcttccgcg ccttcggcga acgtgctgaa cttgcacagg
     2581 tcgaggctgt cggtgaaggc gaacagatcc tggaatgtct tgaccagctc gcccttgccc
     2641 ttccaggcca gggggtcggt cttgagcggg atcagtccca gctcgctggc gggcgtgtag
     2701 ccgcgcaggt ggcacgcgcc gcggttgctg gtggcgtagc caatgcccat gcccttcatg
     2761 ccgcgcggat cgtaggcggg aatggcctgg cctttgaccg tcatgctgat ctcaggatgg
     2821 ccgaagtgct gagctgcgcg cgcagtgccc tcggccagca cgtcgccgat gccctcgcgg
     2881 aaggcgatct tgcgcacggt ctcgaccatg cccatgacat cgccccatct caacccgccc
     2941 gtgccgttgg tgtagcctct ctggctggct tccatataca tggacagcac gttgcccatc
     3001 tcgatggtgt ccaggccata gtcgttgcac acgttaatca gcttggcgat gcccgccgag
     3061 tcgtcgttgt cgcagttggc gccgaaggcc caaaccgact cgtattcctg gctctccatg
     3121 cgcagaccgg cgaagggtcc ctcttcgatc tccacttcct tcttgcaggc gaccgggcag
     3181 gcgtggcagg tcgggtcgtc taccaggatg tgctccttga cccattcgcc gccggtgcgc
     3241 tcggccttct caccgaacga cgtgagctgg ctgttgcggg tcggcagcgc gcccatgttg
     3301 ttggcgatgt tggtgagcac gttcgtgccg tagaccgaca ggccaccctt gcgcgggctg
     3361 gtgacgttct tctcgtccat gatcgtcgcc agcgcccggt cgtgcgccgg cttccaggcc
     3421 gcgcggtcct tggccttgtg catcttcttg agagccttca cgacgatggc cttcaggttc
     3481 ttgctgccgc cgacgcagcc ggtgccgccc cgcccgctgg agcggtcatt ctcgttcatc
     3541 caattagcga agcgcgccag tctctctccg cccgggccga tggcgattac ggtcaggtcc
     3601 ttctcgccgt acttctcctg aaagaacttc accgtgtcgt gcacgccctt gccccatacg
     3661 tctgaggcgt ccaggatttc caccgcgccg tcgtggacgt aggcgtacac gggcttgtcg
     3721 gcctttccct tgaagatcag cccgtcgaag ccggcccagc gcaggcgcgc cgccgaccag
     3781 ccgccgtggt gactgtccgt gatcgtgccg gtgagcggtg acttggtcac gaccgccatg
     3841 cgcccgctca tgttcgcctc tgagccggtg agcggcccgt tcatgaagca caggatgttg
     3901 tcaggcgaca gaggatcgac ctgcgggccg ttctcgaaga cgtatctgtc gcccaggccg
     3961 cgcccgccga tgtacttgcg cgcccactcc tctggaatct cctcatacgt gacgtttcgc
     4021 gtcgtgaggt cgatgcgacc gatcttgttg gcgtatccgc ccaactcagt catgacgttg
     4081 tctccttatc ttgtcgt
//
LOCUS       DBNHXB010000148         5453 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_871669, whole genome shotgun sequence.
ACCESSION   DBNHXB010000148 DBNHXB010000000
VERSION     DBNHXB010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_871669"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="AC162G_05725"
     CDS             complement(<1..776)
                     /locus_tag="AC162G_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5075345.1"
                     /translation="MNYGFLIDNRKCIGCHACSTACKSENEVPLGVNRTWVKYTEKGV
                     FPDTRRYFQVTRCNHCADPPCVHICPVTAMLQRPDGIVEFDSSRCIGCKACMQACPYD
                     AIYIDPETHTAAKCHFCAHRVEVGLEPACVIVCPEHAIIAGDLDDPNSEITRAIATQQ
                     VRVRKPEQDTGPKLFYIDAEEVSIVPALARQDRGMLWATWPDHGAGDWRGPIHIGDGN
                     MASALLRSAAAPRETYNAPHKIPWHWQVPAYLVAKSIGAGA"
     gene            complement(934..1911)
                     /locus_tag="AC162G_05730"
     CDS             complement(934..1911)
                     /locus_tag="AC162G_05730"
                     /EC_number="2.3.1.-"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF046470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIR5075346.1"
                     /translation="MPTAIRLLNLGPAHWLRTQSVYHALAETMTVETPDTIVLTRPSQ
                     SYLCIGYHQELDSVLDRATCRQLSLPIVRRRVGGGTTYLDANQLFYQYVFHHTRVPAT
                     VERLYAALLAAPVAALRALGLDAELRGENEIEVDGQRIAGIGGGRIGEAAVVVGNVLL
                     DFDYDRMARVWRAPSEPFRHLAASALREHVTTLRAKAPRPMAPADMEARLLVEFPAVL
                     ARPLERGELTKEEWGEVESIEKRLMSEEWLSLHANGGQPMTTLKISRGVFIRSAEAEV
                     NGWHVRAALRARDDEIVQAILESEPARDWSEAEARLRGTKIRDWTRAVL"
     gene            complement(1916..2998)
                     /locus_tag="AC162G_05735"
     CDS             complement(1916..2998)
                     /locus_tag="AC162G_05735"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5075347.1"
                     /translation="MAKVTLSSKEQAEQLQISPDYVRISMAAAIELGLKPGRMFRDVH
                     CGCINLLQNYPEGCYANCTYCGLARDRPGVPEDNTFIRVAWPLYPTDLVAEKIAKHQE
                     HVGRVCIAQVQDHRAYDDLIDMTRRVRRLSDVPISALVSATTLSEERLAHIKDAGADI
                     IGVGLDAASEEVFHLTRGRGAKGPHDWNYHWHIVRAARRLFGPMKVNCHIIVGLGETD
                     RDLVDLFYRLKSEQIAGYLFSFNPEPGTTMQHVPRAPIPRWRRIQLVKHLIENYELPR
                     EAIEFDSECNIVRLNAPDLMVEVAVNTGMPFMTKGCPDKNGVMACNRPYGSYRPGEEF
                     RDYPFKPETDDLIIIRQQMRLEEIWQ"
     gene            complement(2998..3966)
                     /gene="lipS1"
                     /locus_tag="AC162G_05740"
     CDS             complement(2998..3966)
                     /gene="lipS1"
                     /locus_tag="AC162G_05740"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF053742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LipS1 family lipoyl synthase"
                     /protein_id="HIR5075348.1"
                     /translation="MPDTAISDRQLPITLSSFDLRRAHFPDEIVFHTPGLKRFKTSEY
                     GGQDAAQFVSVSVTGTACALACEHCKTHILEGMTALPKFGGSLFDLCGELAERGARGV
                     LISGGCDRHGRVPLLRHIPDLIRARRELNLTIRIHPGLPDEETVAGLAEVGIDGALVD
                     IIGDDATIHQVYHLDTTIAEYEAVLECLERYHVPAVPHIILGLHFGQMRGEWMALDMI
                     ARHARKFLVLVILMPLTGTPMVDVAPPPVSEIAGFFALARRTLPATPVMLGCARPLGK
                     VKAEIDLAAVNAGLNGIAYPAEGVVAYAQSCGLTPKFVDACCGVQW"
     gene            complement(3999..5171)
                     /locus_tag="AC162G_05745"
     CDS             complement(3999..5171)
                     /locus_tag="AC162G_05745"
                     /EC_number="2.3.1.-"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF046470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIR5075349.1"
                     /translation="MTDHSRWRLITDDNVSASFGLAADECLAERVGAGESRPALRLYT
                     YRSHCALVGRFQNLENEIHRDYCEAHGIALNRRPTGGGAIIMGADQLGVALTIPGRGD
                     DSYSHARERMAQFSQGIVNALQSLGVRANFRRKNDIEVNGRKIVGLGIYRAHSQARPE
                     SASSAPSVLQRSGLLFHASLLVGLDIPLMLRVLKTPFEKISDKEIATVADRVTTVRRE
                     IGREIELSEVRARVAEGYAAAFGVSLLPGDFTADERRAIAARERSKYVTAEWVNQTTS
                     VPDALGSAKVKTPGGLLDVRATLAGKTLKAVFIGGDFFAAEAAIADLESSLRWHSADP
                     EAVAATLAHAYAGRADDLTALPLDSLTTAVQQAIRRARLAESAARADPYGCFVNPA"
     gene            complement(5206..>5453)
                     /locus_tag="AC162G_05750"
     CDS             complement(5206..>5453)
                     /locus_tag="AC162G_05750"
                     /EC_number="1.4.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF013743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HIR5075350.1"
                     /translation="LEAAKMVGDLIAPVSGTIATRNEEVLRDPTHVNRDPYGAGWLLQ
                     LTPSDWARESTELVHGDGLTAWVEAEMERYRSQGWID"
BASE COUNT          942 a         1664 c         1814 g         1033 t
ORIGIN      
        1 gccccggcgc cgatggattt ggcgacgaga taagccggga cttgccagtg ccacgggatc
       61 ttgtgcggcg cgttgtacgt ctcgcgcggc gcggcggccg agcgcaggag cgcgctcgcc
      121 atgttgccgt cgccgatgtg gatcggcccg cgccagtcgc ccgccccgtg gtcgggccat
      181 gtcgcccaca acatgcccct atcctgcctg gccagcgccg ggacgatgga cacctcctcg
      241 gcgtcgatgt aaaacagttt gggcccggtg tcttgttccg gcttgcggac ccgaacttgc
      301 tgcgtggcga ttgcccgcgt gatttcggag ttgggatcat ccagatcacc ggcgataatc
      361 gcgtgttccg ggcagacgat cacgcatgcc ggctcgagac ccacctcgac gcgatgggcg
      421 cagaaatggc atttggccgc cgtgtgggtc tcgggatcga tgtagatcgc gtcgtacgga
      481 catgcctgca tgcaggcttt gcagccgatg cagcgcgacg aatcgaactc gacaatgcca
      541 tccgggcgct gaagcatcgc cgtgacgggg caaatgtgga cacatggcgg gtcggcgcag
      601 tggttacagc gcgtcacctg aaagtatcgg cgcgtgtccg ggaagacgcc tttctcggtg
      661 tacttgaccc acgtgcggtt gacgccgagc ggcacttcgt tctcggactt gcaggccgtc
      721 gagcaggcgt ggcagccgat gcacttgcgg ttgtcgatca ggaagccgta attcatcgcg
      781 tctccgcaga cttgccctga gcgcagtcga agggacggtc acgccagggg aaaacccagc
      841 ggctccttgc gaagcacccc gaggcgaagc ggagtgggct tgccgccgat ttgtcgggcc
      901 tgggagcctg gccggctggt tttccgacaa gaactacaat acggcccgcg tccagtctcg
      961 aatcttcgtc ccccgcagcc gggcttcggc ctcgctccaa tcgcgggccg gctcgctttc
     1021 caggatggcc tgcacgatct cgtcgtcgcg cgcacgtaaa gcggcgcgaa cgtgccagcc
     1081 attcacctcg gcctcggcgg aacggatgaa aacgccgcgc gagattttga gtgtggtcat
     1141 cggttgcccg ccgttcgcgt gcaagctcag ccattcctcg ctcatgagtc gcttttcgat
     1201 agactcgacc tcgccccatt cctctttggt cagctcaccg cgctccagcg ggcgagcgag
     1261 gacggcggga aattcgacaa ggaggcgtgc ttccatgtcg gcgggcgcca tcgggcgcgg
     1321 cgctttggcc cgaagcgtcg tgacgtgttc ccttagggcg ctggcggcca aatggcggaa
     1381 tggctccgag ggagcgcgcc agacgcgggc catcctgtcg tagtcaaagt cgagcagcac
     1441 gttgccgacg acgacggccg cttcgccgat acgcccaccg ccgatgccgg cgattcgctg
     1501 accgtcgact tcgatctcgt tctcgccgcg cagctcggcg tccagtccca gtgcgcgcag
     1561 tgcggcgaca ggcgctgcca acaatgcagc gtacagccgc tccactgtgg ccgggacgcg
     1621 ggtgtgatga aaaacgtact ggtaaaacag ttgattcgcg tcgagatagg tcgtaccgcc
     1681 gcccacacgc cgccggacga tgggcagact cagttgccgg caggtcgcgc ggtccaggac
     1741 cgagtcaagc tcctgatggt atccaatgca tagatacgat tgggacggcc gggtgaggac
     1801 gatggtgtca ggcgtctcca ccgtcatcgt ctcggccagg gcgtgataga ccgactgggt
     1861 gcggagccag tgggccgggc cgagattcaa gagacggatg gcagttggca tgctgtcatt
     1921 gccagatttc ttccagccgc atctgctgcc ggatgatgat caagtcgtca gtctccggct
     1981 tgaacggata atcgcggaat tcctcgccgg ggcgatacga gccatagggc cggttgcagg
     2041 ccatcacgcc gtttttatcg gggcagccct tggtcataaa cggcatgccc gtgttcaccg
     2101 ctacttcgac catcagatcg ggtgcgttta gccggacgat gttgcactcg gaatcgaact
     2161 cgatcgcctc gcgcggcagc tcgtagtttt cgataaggtg cttgaccagt tgaatccgcc
     2221 gccagcgggg gatcggcgcg cgtggcacgt gctgcatggt cgtgcctggc tcgggattga
     2281 acgagaacag gtatcccgcg atttgctcgg acttgagtcg atagaagaga tcgaccaggt
     2341 cgcgatcggt ctcgccgagg ccgacgatga tgtggcaatt gactttcata gggccgaaca
     2401 ggcggcgcgc ggcgcgcacg atgtgccagt ggtagttcca gtcgtgcggc cccttcgcgc
     2461 cacgtccgcg cgtgaggtgg aaaacctcct cgctggctgc atccaatccg acgccgatga
     2521 tgtccgcgcc ggcgtctttg atgtgtgcca gccgctcttc gctcagcgtc gtcgccgaga
     2581 cgagcgcgga aatcggcacg tcgctcaggc ggcgcacgcg ccgagtcatg tcgatcaagt
     2641 cgtcataggc gcggtgatct tggacctgtg cgatgcatac ccgcccgaca tgctcctggt
     2701 gcttggcgat tttctccgcg acgagatccg tcgggtacag cggccaggcc acgcgaatga
     2761 acgtgttatc ttcaggcacg ccaggccgat cgcgggccag cccgcagtac gtgcagttgg
     2821 cgtagcaccc ttccgggtag ttctggagca ggttgatgca cccgcagtga acatcgcgga
     2881 acatgcgccc gggcttaagg ccgagttcaa tcgccgccgc catgctgatg cgcacgtagt
     2941 caggcgagat ttggagctgc tcagcttgct ctttggacga tagcgtgact ttagccatta
     3001 ccattgcact ccgcaacagg cgtccacgaa tttcggcgtc aggccacacg attgggcata
     3061 ggcgacgacg ccttcagccg gataggcgat gccattcaga cccgcattca ccgccgcgag
     3121 gtcaatttcg gctttgacct tccccagcgg gcgcgcgcag ccgagcatga cgggtgtggc
     3181 tggcagcgtg cggcgggcca gcgcgaagaa gccggcgatt tccgacacgg gcggcggggc
     3241 cacgtcgacc atgggcgtgc cggtgagtgg catgaggatc accagcacga ggaatttcct
     3301 ggcgtgtcgc gcgatcatgt cgagcgccat ccactcgccg cgcatctggc cgaagtgcag
     3361 gccgaggatg atgtgcggca cggcgggcac atggtaccgc tccaggcact caagcaccgc
     3421 ttcgtattcg gcgatggttg tatcgaggtg atagacctgg tggatcgtcg cgtcgtcccc
     3481 gatgatgtcc accagcgcgc cgtcaatccc gacttcggcc aggccggcga cggtttcctc
     3541 gtcgggcagg ccggggtgga tacggatggt gaggttgagt tcgcgccggg cgcggatcag
     3601 atcggggata tggcgcagca gcggcacccg cccgtgccgg tcgcatccgc cggaaatcag
     3661 cacgccgcgc gcgccgcgtt ccgcaagttc gccgcacaag tcaaatagtg agccgccgaa
     3721 cttgggcagc gcggtcatgc cttcgagaat gtgcgtcttg cagtgctcgc aagcgagtgc
     3781 acaggctgtg ccggtcacgc tgaccgaaac gaactgcgcc gcgtcttgcc cgccgtattc
     3841 ggacgtcttg aaccgcttga gtccgggtgt gtggaatacg atctcgtccg ggaagtgcgc
     3901 gcgacgcagg tcaaaggagg aaagggtaat tggtaattgg cgatcggaga ttgcggtgtc
     3961 aggcatgagt ggcttctgcc tcaattacgt tttacgcttc aggctggatt cacgaagcac
     4021 ccgtacgggt cggcgcgcgc ggccgattcg gccagccgcg cgcggcgaat cgcctgctgc
     4081 accgccgtcg tgagcgagtc aagcggcagg gcagtcaggt catcagcccg gccagcgtaa
     4141 gcgtgggcca gcgtcgcggc gaccgcttct ggatcggccg agtgccagcg cagactggat
     4201 tccaggtcgg cgatcgcagc ttcggcggca aagaaatcgc caccgatgaa caccgctttc
     4261 agagtcttac cggccagtgt ggcgcgcaca tccagcaggc cgccgggtgt tttgactttc
     4321 gccgagccga gtgcgtccgg cacactggtc gtctggttca cccactcggc ggtgacatac
     4381 ttgctgcgtt cgcgcgcggc aatcgcgcgg cgctcgtcgg cggtgaaatc gccgggcagg
     4441 agcgagacgc cgaacgcggc ggcgtagcct tcggcgacgc gcgcgcggac ttcgctcaat
     4501 tcgatctcgc gcccgatttc gcggcgcacg gtcgtgacac ggtcggcgac ggtggcgatt
     4561 tccttatcgg agatcttttc aaagggtgtc ttgagtacac gcagcatgag cgggatgtcg
     4621 aggccgacca gcagtgaagc gtgaaagagc aagcccgaac gctgaagcac tgagggcgct
     4681 gaggacgccg attcgggccg agcctggctg tgcgccctgt aaattcccag gccgacgatt
     4741 ttccgtccat ttacttcgat gtcattcttg cgccggaaat tggctcgcac gccaagtgat
     4801 tggagggcat tgacgatacc ttgcgagaac tgcgccatgc gttcgcgtgc gtggctgtac
     4861 gagtcgtcgc cgcgtccagg aatcgtcagc gcaacgccga gctggtctgc gcccatgatg
     4921 atggcgcctc cgcccgtcgg ccggcgattg agcgcaatgc catgcgcctc gcagtagtca
     4981 cggtggattt cattctctaa gttctgaaag cggccgacga gcgcgcagtg tgagcgatac
     5041 gtgtagagcc gcagggccgg acggctctcg ccggcgccga cgcgttcggc caggcattcg
     5101 tcggcggcga gcccgaagga ggcggagacg ttatcgtcgg tgatcaggcg ccagcgtgag
     5161 tgatcagtca tcagtcatca gttatcagtc atcagttatc agtagtcagt caatccagcc
     5221 ttgcgatcgg tatcgctcca tctccgcttc cacccatgct gtcaggccat ctccgtggac
     5281 gagttcggtg gactcgcgcg cccaatcgga aggtgtgagt tggagcagcc agccggcgcc
     5341 gtagggatcg cggttgacgt gggtcggatc gcgcaggact tcctcgttgc gggtggcgat
     5401 cgtgccgctc accggcgcga tgaggtcacc gaccattttc gcggcttcga gcg
//
LOCUS       DBNHXB010000149         4618 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3556128, whole genome shotgun sequence.
ACCESSION   DBNHXB010000149 DBNHXB010000000
VERSION     DBNHXB010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3556128"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2037
                     /locus_tag="AC162G_05755"
     CDS             <1..2037
                     /locus_tag="AC162G_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR5075351.1"
                     /translation="ASGILFTANPMNGQRDQAVINATWGLGEAIVGGLVTPDTFTVNK
                     ATGQVMQREIADKQVMTVRVEGGTREQPVPESLRRVPVLDEDTAGQLNRLGVRIEQLY
                     GMPMDIEWTLTDGRFAIVQARPITALAETPIEWKLPNPKGVYMRGSVADLMPDPLSPL
                     FVSLGIPSLKKQMYPLGRRLTRSEPVLPDDYITSINSYAYVNTTLPARSWWWMVTGLL
                     RSYPRLLRIMVPLWRDELLPEYQALVAQQQDRVPGEMTVDELWRDAQGIVDAAMSYVI
                     ALLFATMGASAGSELLLTNVYNRMAKKDGDPPATALLMGWNNVPVRAEKSLYDLATWC
                     REREALAAYLLATPSKQLVGQLAGGQPPTEVNAEDWKELQARFEQHLKQFGHIVFQLD
                     FSEPLPLDDPSPMLGNVKMYLRGEGVNPHERQQAGEAKRVQTAETMLKRLRGLKRWAF
                     RKALNWGQSLSEVREDALAYIGLGYPMLRAMLRELGRRFVEAGIIRQADDIFYLEKEE
                     IDTCVANLEHGAAPGDLSGRVEERKAFVKKAGQATPPPMMPMKKRVMGIKTDVFVAAS
                     EESQTGNILKGVATSAGKVTAPARVLRGPEDFDQMRPGDVLVAGTTTPAWTPLFAMAS
                     GIVTDIGGPLSHGSIVAREYGIPAVMGTGVATKRIRSGQMITVDGSAGTVTLNA"
     gene            2105..3328
                     /locus_tag="AC162G_05760"
     CDS             2105..3328
                     /locus_tag="AC162G_05760"
                     /inference="COORDINATES: protein motif:HMM:NF018485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5075352.1"
                     /translation="MFARNRNLLILSFTLIVVMLGFGMVIPIFPFYIDRLGAGGSALG
                     LLVATAALTEFTFGPVWGSVSDRTGRKPILMIGVVGYGLSLLLFGLSTELWMLFAARA
                     LSGVLSSATMSTAMAYIGDSTSQKERGGGMGALGAAAGLGVILGPGIGGWLAGDSLST
                     PFFIGAGLSLVSVLMIGLLLPETLPREARAPAGGKIRLMNLRELRRALTKGSPIRFLL
                     LLAFIGTFGTANFEAIFGLFMLEQHGYGPEQVGGILTLVGIVAVIGRGVLTGWLTQWW
                     GEATVIKASLLAGAVGFVLLLLANDYATVLLTTGLFVLITTFLRPAIHSLTSQRAMIG
                     QGAAMGLSNSFVSLGRVAGPIWAGAIFDLNPNYPYMCGAAILLLGFVMSAIWIVRDQK
                     VAAPEYPLSNTPFGD"
     gene            3436..3678
                     /locus_tag="AC162G_05765"
     CDS             3436..3678
                     /locus_tag="AC162G_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075353.1"
                     /translation="MTKRPSLSEPAAYRMRVKGVLDARWAEQFEGMTLIVDQGDTILS
                     GVVADQPALHSLLVKIRDLGLVLISVNRIDADSEEA"
     gene            3769..4233
                     /locus_tag="AC162G_05770"
     CDS             3769..4233
                     /locus_tag="AC162G_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075354.1"
                     /translation="MIEQGALQTVYILFAFAVQIILIADFAARVWKSALELRWGWLIY
                     TLVGVAGLLLGVLLVVSRQPAYLAAGPLLLAIWAAVGYTVDVWRPVNWRSPPRWRIFI
                     PYVGLYVTALLVMWVSMWFIGLAYWIAFGAMYAVHTGLNIYSHFKTRETGTV"
     gene            4255..>4618
                     /locus_tag="AC162G_05775"
     CDS             4255..>4618
                     /locus_tag="AC162G_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009630296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5075355.1"
                     /translation="MTEIAIRIEGVTRDFGSVRAVDHLSLEVPSGIIYGFLGPNGAGK
                     TTTINLLLGLLEPTAGRAEVLGFDTRTQADEVRARTGALLEHTGLYEQMSAEDNLEFY
                     GRVYRMSPADRQARIKELL"
BASE COUNT          825 a         1361 c         1523 g          909 t
ORIGIN      
        1 gcctcgggca ttctgttcac ggcgaacccg atgaacggtc agcgcgatca ggccgtcatc
       61 aatgcgacgt ggggactggg cgaagccatc gtcggcggcc tggtcacgcc cgacacgttc
      121 acggtgaaca aggcgaccgg tcaggtgatg cagcgcgaaa tcgccgacaa gcaggtgatg
      181 accgtgcgcg tcgagggcgg cacgcgggag cagcccgtgc cggaaagcct gcgccgcgtg
      241 ccggtgcttg acgaagacac ggccgggcaa ctcaaccggc tgggcgtgcg catcgagcag
      301 ttgtatggga tgccgatgga catcgagtgg acgttgaccg atggcaggtt cgccatcgtc
      361 caggccaggc cgatcacggc tctggcggaa acgcccatcg aatggaagtt gcccaacccg
      421 aagggcgtct acatgcgcgg cagtgtggcg gacttgatgc ccgacccgct cagcccgctg
      481 ttcgtatcgc tcggaatccc atcgctgaaa aaacagatgt acccgctggg gagacgcctg
      541 acgcgctctg agcctgtcct gccggatgac tacatcactt ccatcaacag ctatgcctac
      601 gtgaatacca ccctgcctgc ccggtcatgg tggtggatgg ttacggggct gctgcgttcg
      661 tacccgcgcc tgctgcggat catggttccg ctctggcgcg acgaactgct cccggaatac
      721 caggctcttg ttgcccaaca acaggacagg gtgcccggcg agatgaccgt ggacgaactc
      781 tggcgagacg cacagggcat cgtggatgcc gccatgagtt acgtcatcgc gctgttgttc
      841 gccacgatgg gcgcttcggc tggctcagaa ctgctactga cgaatgtgta caacaggatg
      901 gcgaagaagg acggcgaccc gcccgccacg gctctgctga tgggatggaa caacgtcccg
      961 gtgcgggcgg agaagtcatt gtatgacctg gcgacgtggt gccgcgagcg tgaggcgctg
     1021 gctgcttacc tgctggccac accttccaag caactcgtcg ggcaactggc aggtgggcag
     1081 cccccaaccg aggtcaatgc ggaagactgg aaagaactgc aagcgcgctt cgagcaacat
     1141 ctgaagcaat tcgggcacat cgtcttccag ttggactttt ccgagccatt gccgttggac
     1201 gatccctcgc ccatgctggg gaacgtcaag atgtatctgc gcggcgaggg agtgaatccg
     1261 catgagcgtc agcaggccgg tgaagcaaag cgagtgcaga ctgctgaaac gatgctcaag
     1321 cgcctgcgag gtctgaagcg gtgggccttc cgcaaggcgc tcaattgggg gcaatccctg
     1381 tcggaagtgc gcgaggacgc tttggcgtat atcggtctgg ggtatcccat gctgcgcgcg
     1441 atgctgcgcg agctgggccg gcgcttcgtc gaggcaggca tcattcgaca ggcggacgac
     1501 atcttctacc tggagaaaga ggaaatcgat acgtgtgtgg cgaacctgga acacggcgcg
     1561 gcgcccggcg atttgtctgg ccgtgtggaa gaacgcaagg ctttcgtgaa gaaggccggg
     1621 caagccactc cgccgccgat gatgccgatg aagaaaaggg tcatggggat caagaccgat
     1681 gtcttcgtcg ccgcttccga ggaatcacag acggggaaca tactcaaagg agtggccacc
     1741 agcgccggta aagtcaccgc gccggcccgc gtgctgcgcg gccccgagga cttcgatcag
     1801 atgaggccgg gggatgtgct ggtggcgggc acgaccacgc ccgcctggac gccgctgttt
     1861 gcgatggcat ccggcatcgt gaccgacatt ggcgggccgc tcagtcacgg ctcgatcgtc
     1921 gcgcgggagt atggcatccc ggccgtgatg ggcacgggcg tggcgaccaa acggattcgg
     1981 agcgggcaga tgatcacggt cgatggaagc gcggggacgg tcacattgaa tgcgtgaaac
     2041 gtaaagcgtg aaacgtaatg gcctcacgtt ttacgttgca cgttttattt tggagtcgtt
     2101 gtccatgttc gccaggaatc gcaacctcct gattctctcc tttaccctga tcgtcgtgat
     2161 gctgggcttc gggatggtca ttccgatctt cccgttctac atcgatcggc tgggcgcggg
     2221 cggcagcgcg ctcggcctgc tcgtcgccac ggctgcgctg accgagttca ccttcgggcc
     2281 ggtgtggggt agtgtgtccg atcggacggg ccgcaagccg atcctgatga tcggcgttgt
     2341 gggctatggt ctctcgctac tgctgttcgg actttcaacc gaattgtgga tgttgttcgc
     2401 tgcccgcgcg ttatcggggg tgttgtcctc ggcaacgatg tctacggcga tggcctacat
     2461 cggcgacagc acgtcgcaga aggaacgcgg cggcgggatg ggcgcgctcg gcgcagcggc
     2521 cggactgggt gtcattcttg ggccggggat cggcggctgg ctggcaggcg actcgctttc
     2581 gacacccttc ttcatcggcg caggattatc actcgtgtcc gtattgatga tcggcttgct
     2641 gctgcccgaa acgcttccac gcgaggcgcg cgctccggcc ggcggcaaga tccgcctgat
     2701 gaacttgcgc gaactgcggc gggcgttgac caaaggcagc ccgattcgct tcctgctact
     2761 tcttgccttt ataggcacgt tcgggacggc caacttcgaa gcgatcttcg ggttgttcat
     2821 gctggagcaa cacggctatg gcccggagca ggtgggcggc atcctgaccc tcgtcggcat
     2881 cgtcgccgtg atcggccgag gcgtgctgac cggctggctg acccagtggt ggggcgaggc
     2941 gacggtcatc aaggcgtcgc tgctggccgg cgcggtgggc ttcgtgttgc tgctgctggc
     3001 gaatgattat gcgacggtat tgctcacaac cggtttgttc gtgttgatca ccactttcct
     3061 gcgcccggcc atccactcgc tgacgtcgca gcgggcgatg atcggccagg gcgcggcgat
     3121 gggtctgagc aactcgttcg tcagtctggg ccgcgtcgcc ggacccattt gggccggggc
     3181 gatcttcgat ctcaatccga actatcccta catgtgcggc gcggccatcc tgttgctggg
     3241 attcgtgatg agcgcgatct ggatcgtgcg ggatcagaag gttgccgctc ccgaataccc
     3301 cctgtcgaat acccctttcg gggactgaca gaaacggccg ctgctcataa actggaacca
     3361 gactgcttcg gtggctgtcg tgccggcact tgactggcgc gtgccacact ctctcgctca
     3421 aaggaaacgc aacgcatgac caaacgcccg agtttgtcag aacctgctgc gtatcggatg
     3481 cgcgtgaagg gtgtgctgga tgcgcgctgg gccgagcagt tcgagggcat gacgctgatc
     3541 gtggatcaag gcgacacgat cctgtccggc gtcgtcgccg atcaaccggc tctgcacagt
     3601 ctgctggtca agatccgcga cctcggtctg gtgctgatct cggttaatcg catcgacgcc
     3661 gattctgaag aggcctgaat ccattggttg gccgcgccgg gtacggcgcg acgagccgaa
     3721 aatacagatg acggcgatga tcactaattc acttgcagga ggtgcagcat gatcgaacaa
     3781 ggcgctctcc agacggtgta catcttgttc gcgttcgccg tccagatcat cctcatcgcc
     3841 gatttcgctg cgcgtgtctg gaagtctgct ctcgaactac gctggggttg gctgatctac
     3901 acactcgtcg gcgtagcggg gttgcttctg ggcgttctgc tcgtggtcag tcggcagccc
     3961 gcgtatctcg cagccgggcc actgcttctg gcgatctggg cagccgtagg atacaccgtc
     4021 gacgtctggc ggccggtgaa ctggcgcagc ccgccccgct ggcgaatctt catcccttac
     4081 gtgggcttgt acgtgacggc gttgctggtc atgtgggtct ccatgtggtt catcgggctt
     4141 gcctactgga ttgcgttcgg cgcgatgtat gccgtgcaca ccggcctgaa catctactcg
     4201 cacttcaaga cccgcgagac gggaactgtg tgaggcacac aggaggttat cgtcatgaca
     4261 gagatcgcaa tccgaatcga aggcgtcacc cgcgacttcg gctcggtgag agccgttgat
     4321 cacctctcgc ttgaagtccc aagcgggatc atttacggct tcctcggccc aaacggggcc
     4381 gggaagacga ccaccatcaa cctgctgctg ggcctgcttg agcccaccgc gggtcgagcc
     4441 gaggtgttgg gcttcgacac gcgtacgcag gccgacgagg tgcgcgcccg caccggcgcg
     4501 ttgctggagc acaccggcct gtacgagcag atgagcgccg aggataacct ggagttctac
     4561 gggcgcgtgt atcgcatgtc gcccgccgac cgccaggcgc gcatcaagga gctgctca
//
LOCUS       DBNHXB010000150        14973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_201096, whole genome shotgun sequence.
ACCESSION   DBNHXB010000150 DBNHXB010000000
VERSION     DBNHXB010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 14973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14973
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_201096"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="AC162G_05780"
     CDS             <1..416
                     /locus_tag="AC162G_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009990994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR5075356.1"
                     /translation="VKVRRFVAVDDCGVRINPMIVEGQIHGGLTMGFAPALMEEISYD
                     ENGNRYGSTFMDYMLPTAVETPRWETGETVTPSPHHPIGAKGVGESATVGAPAAIANA
                     VVDALWHLGVRHIDIPITAPKVWALLEEKGAALEE"
     gene            670..1089
                     /locus_tag="AC162G_05785"
     CDS             670..1089
                     /locus_tag="AC162G_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR5075357.1"
                     /translation="MPIRTSEAVWSGTLREGQGTMKLGSGTYAGAYTFASRFEEGPGT
                     NPDELIGAAHAGCFSMFLSAVLTNAGYTPTRIRTTATVHLGEGPKITSIELDTEAQVP
                     NLDEKTFLEAAEESKNKCPVSQALAGTEIRLNAKLVG"
     gene            1144..2148
                     /locus_tag="AC162G_05790"
     CDS             1144..2148
                     /locus_tag="AC162G_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR5075358.1"
                     /translation="MIIDLLERAAELSRAGQSFALATVVRAERPTSAKPGAKAIVTVD
                     GALTGWVGGSCAQPTVVREALKALQDGEPRLVRLCPPEKLGSAPQEGVVEVALTCISG
                     GTLEVYIEPHLPRPHLVVVGHLPIAEALSRLGSALDYDVTVMGLDVTPDRFPDADRIL
                     DRLDFSSLKLTPQAYVVVATHGNYDEDALEGALRSDAAYVALVASRARAEAIRQYLRE
                     SGLSDAALVRLKAPAGLDLGAVTPEEIALSILAEIVQLRRPPTRQSHAPPPVVQPLEA
                     TDPVCGMLVDIATARYTSHYGSRVYYFCSAGCQRSFEKQPEKYVTASDTTPVAHHTNS
                     "
     gene            2163..2606
                     /locus_tag="AC162G_05795"
     CDS             2163..2606
                     /locus_tag="AC162G_05795"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5075359.1"
                     /translation="MRYEGTITISAPRGVVWVFLTDPLQVAQCAPGVESVETLVPDYK
                     FRAIAAIGFGSVKARFTGEVEFLELEPPTRAKLKAHGDAPGSAADVISEMFLTDGPDG
                     TTEMRWSVDIVVLGTLASLAARLMSSITQKLTAQFFECLKSKIEA"
     gene            2631..4262
                     /locus_tag="AC162G_05800"
     CDS             2631..4262
                     /locus_tag="AC162G_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HIR5075360.1"
                     /translation="MRGTMMDYPLTLQHFLERSHKMSPKKEIASKTATGMHRYTYADF
                     YARVHRLANALADLGVRRGDRVATFAWNHYRHLELYFAIPCMGAVLHTLNIRLFPDQL
                     VYVINHAEDKIIFVDATLLPALQRVQDQIKHVERFVIMTDTGEVPETTLSPTGEYESL
                     LSAARPEFPFPKLDELDAAGMCYTSGTTGNPKGVVYTHRAIFIHSFAEAMADSLAVSE
                     RDTVMPVVPMFHVNAWGLPFTGTMVGAKQVFPGPHLQPRDLAELIQNEKVTLAAGVPT
                     LWIGLYSLLEREQYDLSSLRTLVVGGSAAPRTLIENFEKNFGVPITHAWGMTEMTPLG
                     TVSKLKAELLDAPEEVRFAYRAKQGRAMPGVEVKVVDEAGQAVAWDGRTMGELWVRGP
                     WIASSYYNDPNLSDVFAPDGWFRTGDVVTLDPEGYMEITDRTKDLVKSGGEWISSVAL
                     ESALMGHPKVLEAAVIAIPHEKWQERPMAVVVPKPDQAESITKDELIEFLRPKFAKFW
                     LPDDLVFIKEMPKTSVGKFDKKVLRERFKEHRTSA"
     gene            4306..5343
                     /locus_tag="AC162G_05805"
     CDS             4306..5343
                     /locus_tag="AC162G_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HIR5075361.1"
                     /translation="MKFGPNPLDQAEGQILGHNIAGLDGRRLLRKGKPLTRDDVEVLR
                     RLGRTTVYVAALEPDDVNEDTAAHRVATAALGGNLRLSGSASGRVNFLATHLGVLRVD
                     ADRLARINACQDITFATLASHSAVRPRQIVATVKVIPYGVPNSVVRAAEAIASDDGPL
                     VRVDALEPRPVSLVFSGSLSIRDKLAADFAPLRHRVEALGSTILSTDYVPLEDETGEA
                     ALADALSRRRAAGARLIVVAGETAIMDRHDIVPRAVERAGGWVECLGAPVDPGNLLML
                     AYLGDMPILGAPGCARSRKVNVVDWVLPRLLAGDHLTRDDIIALGHGGLLEEAPERPA
                     PRETVEAGGGK"
     gene            5642..6109
                     /locus_tag="AC162G_05810"
     CDS             5642..6109
                     /locus_tag="AC162G_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5075362.1"
                     /translation="MEQSVSITVNGVKRTHPVEPRMLLVHYLRDVLGLTGANVGCDTS
                     QCGSCTVILDGMAVKSCTVLAVQADRSSVMTIEGLAQDGKLHPLQQGFWEKHGLQCGF
                     CTPGMIMTAVDLLQRNPHPSEAEIRHGIEGNICRCTGYQNIVKAIQAAAEMMG"
     gene            6121..8469
                     /locus_tag="AC162G_05815"
     CDS             6121..8469
                     /locus_tag="AC162G_05815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR5075363.1"
                     /translation="MAKYVGASIKRVEDPRFIQGKGNYVANISLPNMAHVAIKRSPYG
                     HARIKKINTKKATALPGVIAVFTGQDLIDGGVGKLPCGWNVPDIKVPTRWPLTVDKVR
                     HVGDGVAIVVAETAYIAHDALDLIEVDYEPLPAVVDARKTTEKGAPLVHDDVPNNISY
                     TWAIGDKEAMEKAFAEAEHVVTLDLVNQRLIPNAMEPRAAVAQWSAATEEMTLWTTSQ
                     NPQPIRLLLSAFTLGIPENRLRVISPDVGGGFGSKIFHYPEEIIVPWVARKINRPVKW
                     VATRTESNMTDSQGRDHVTKSRMALKKDGTITGIHVQTWANQGAYLSTFAPLIPTALY
                     LTLLSGLYKVKGIWGEMWGTLTNTVPVDAYRGAGRPEATYVIERLVDLAARDLGMDPA
                     DIRHKNFIPKDEFPYQTPVAFMYDSGDYDALFHKAMTMADYSALRREQAQARKQGRLV
                     GIGIVGSIEASGPAPSKIAGALGSAVGFWESGVIRVHPSGKVTALTGSHAHGQGHETA
                     FAQVIADELGVSMDDVEIVHGDTGRVPFGMGSYGSRSAAVGGSALVNAAKRVRQKVMK
                     ISAHQLEAAEEDMVYDQESGKIYVHGSPGKAKSFGEVALAAYTAHNLPEGMEPGLEES
                     AWYDPPNFTFPNTCHIVQVEVDRDTGEVTIQRYVAVDDMGKVINPMIVEGQVIGGVVQ
                     GVGQALWEHGIYDENGQLITGSLMEYAVPRAEAFPMIETGRNETPSPHNPLGVKGAGE
                     MGTVTGTVAVANAVMDALAPLGVRHVDMPLTPEKIWRAMHKS"
     gene            8539..9411
                     /locus_tag="AC162G_05820"
     CDS             8539..9411
                     /locus_tag="AC162G_05820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR5075364.1"
                     /translation="MYPASFDYYRPKTVAEAVKLLGQNKNARVLAGGHSLLPQMKLRV
                     TAPSALVDIGRVKGLSDIKATKTALKIGAMTTHATVADSDVVKKLCPILAEAAALIGD
                     VQVRNRGTLGGSLAHADPSADYPTVIVALGATITVTGPKGSRDIAADKFFKDLFTTAL
                     RPAELITSVSVPAYGGMQGMGGAYLTHEHPASSYAVVGVAALVGLKNGACARVSLVVG
                     GATVNPVHAAAAENALTGQAPSEASIAAAADKVAEAIADPLSDTYASGEYRKHLATVL
                     ARRALTMAAERARG"
     gene            complement(9491..9868)
                     /locus_tag="AC162G_05825"
     CDS             complement(9491..9868)
                     /locus_tag="AC162G_05825"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5075365.1"
                     /translation="MAQVDLDQYRAFWRQRSRIEPTPEMQAAARDARHEAQRLSQLLA
                     AEFGAKRVYLFGAFAWGPEMRPDSDLDLAVEGLPRGKLVFAHARLSSQTHYTVDLVLL
                     ERLPEPLRSRILTSGILLYGEQS"
     gene            10109..10531
                     /locus_tag="AC162G_05830"
     CDS             10109..10531
                     /locus_tag="AC162G_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075366.1"
                     /translation="MTVQAITLTVPEQVYEQIRRAAEKAHRPVDEVLAEAVAAVAPVM
                     DTAPERLRSALAHMAYLNDAALWQAARATMTAKQRERLADLHDQQQRATLTPEEQAEE
                     QALLALYRETLLVRAQAAVLLKQRGYNVSDLDQFTPLE"
     gene            10509..10826
                     /locus_tag="AC162G_05835"
     CDS             10509..10826
                     /locus_tag="AC162G_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR5075367.1"
                     /translation="MNLRHSNEFFVHFGGASQRVAETARFRCGYCLASQRIIGPLLEL
                     DHIVPEAHGGTSDEENLFLACPMCNSHKADRREAVDPESYEPSRCCGCTPPVGHRWLA
                     SPY"
     gene            10866..11750
                     /locus_tag="AC162G_05840"
     CDS             10866..11750
                     /locus_tag="AC162G_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5075368.1"
                     /translation="MIIDSIDDLQNALREQLYIADRGLATSIYLALKLNRPLFLEGEA
                     GVGKTEVAKVLTRLLDTDLIRLQCYEGLDVHHAVYEWNYTRQMMHIRLLEARGERPSE
                     AELFGPEFLLRRPLLQAIENAREKSPVLLVDEIDRSDEEFEAFLLEVLSDFQISVPEI
                     GTIKAERPPVVVITSNRTREVHDALKRRCLYYWIDYPSFEKELEIVQAKVPHASAQLA
                     RQVTRFVQELRRMDLYKIPGVAETLDWTAALVALDQRALSPSIVDDTLGALLKYQDDV
                     GKVKGDQARLILDRLGAL"
     gene            11747..12970
                     /locus_tag="AC162G_05845"
     CDS             11747..12970
                     /locus_tag="AC162G_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR5075369.1"
                     /translation="MTSPQEPDPDSGHLLHNLLLFGRLLRGLGLDVNPGRMIDLTHSL
                     AYIDLSHRQDFYHAARSLLVHRHEDIPLFDAAFEAFWRKPEEGWVTVDLPGLSRALRR
                     QRPVIMPPPLRGPDSAGRQDKDDTPAENRPPVLQVTMTYSAREVLRNKDFASLTGEEL
                     DAIKQMMAALVWQLGERRTRRQQPGRGRLLDLRRTIRRSLKYGGEVMEWARREPKHKP
                     RPLVILADVSGSMERYIRLLLHFVYGVTAGLDQPVEAFLFSTRLTRITRELQGRDVDE
                     ALRDVSRTVQDWSGGTRIGEALKTFNFKWGRRVLGRGAVVLLISDGWDRGDIELLRLE
                     MARLQRSVYRLIWLNPLLGSPEYEPLTRGMQAALPYVDDFLPVHSLASLEDLAKRLIG
                     LNERRPERRQFRVLS"
     gene            13064..13504
                     /locus_tag="AC162G_05850"
     CDS             13064..13504
                     /locus_tag="AC162G_05850"
                     /inference="COORDINATES: protein motif:HMM:NF017451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5075370.1"
                     /translation="MFKFERMIAWQKAVEFADHAFDVADRLPQRQQFSFGEQLRRAAL
                     GITNNLAEGSGRRTPGTRRTFYDSGKGSTYEVVSIIAILLRRKEVTQTEFDTFYRMGD
                     ELASIISGLIDATFREEAELGQTPGRLRDEEEQYLTSEDTDSEL"
     gene            13540..14583
                     /locus_tag="AC162G_05855"
     CDS             13540..14583
                     /locus_tag="AC162G_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR5075371.1"
                     /translation="MREVLSDIDRWQAQGKSIALATVVQTWGSAPRGAGAKMALTLDG
                     EISGSVSGGCVEGAVFESGTQVLKTGRPQLLSFGVADETAWEVGLACGGSIRVFVKPL
                     DSAMYDEAHKAVTADRLAVVVTVVKGPDEALGRELMLREDGSLVGSLDAGLDEAAIAA
                     ARSAMEAGQSQPATLSRTDGDPLEIFVDVLLPSPTLVAVGGVHIAIALASIAKALGYR
                     TVVVDPRRAFGSETRFPHVDQLIQEWPDDALAQIGLNRSTAVAMLTHDPKLDDPALKM
                     ALPSPAFYVGALGSNKTQAARRERLLAAGLTEAQVDRLHGPIGLDIGAKTPEEIALAV
                     MAQIVAARNGAAS"
     gene            14676..14747
                     /locus_tag="AC162G_05860"
     tRNA            14676..14747
                     /locus_tag="AC162G_05860"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14708..14710,aa:Gly,seq:ccc)
BASE COUNT         2943 a         4615 c         4833 g         2582 t
ORIGIN      
        1 aggtgaaagt gcgccgcttc gtggccgtcg acgattgcgg cgtccgcatt aacccgatga
       61 tcgtcgaggg acaaatccac ggcggcctga cgatgggctt cgctcccgcg ctgatggagg
      121 aaatttcata cgacgaaaac ggcaacaggt atggcagcac gttcatggat tacatgctgc
      181 cgacggccgt cgagacgccg cggtgggaaa ccggcgagac ggtcacgcct tcgccgcacc
      241 acccgatcgg cgcgaagggc gtcggcgagt cggccaccgt cggcgcgcca gcggcgatcg
      301 ccaatgccgt cgtggatgcg ctgtggcatc tcggcgtgcg gcacatcgac atccccatca
      361 ccgcgccgaa ggtgtgggcc ttgctggagg agaaaggagc cgcgctggag gagtgagtta
      421 gagtatggag ttagagtgtg gagttagagt aaggccggtg ccgtcggcgt gcttgtcatg
      481 ggcgaaaaac tcaacgagaa ggctcaggtc ttccacagtt agagctgccc tctcgcctgc
      541 gcgacgtgaa cgttccggag gacgcactcg ccgccactgc cctggccgcc gtcggcgatt
      601 catcgcagag ggcggaggtc gccgagatac tgcgacgggc gtggtagatt gattgaagga
      661 gatgagacaa tgcctatccg tacctcagag gccgtctggt cagggaccct gcgcgagggc
      721 cagggaacga tgaaacttgg cagcgggacc tacgcggggg cttatacctt tgcctcgcgc
      781 tttgaggaag gaccggggac gaatccggac gaactgattg gggcggcgca tgccgggtgc
      841 ttctctatgt tcttgtcggc tgttctcacg aacgccggct acacgcccac ccgtatccgc
      901 accacggcga cggttcatct cggtgaaggc cctaagatca cctccatcga gctggacact
      961 gaggctcaag tccccaatct cgatgagaag accttccttg aagccgccga agaatcgaag
     1021 aacaaatgcc cggtctcaca ggctttggcc gggaccgaga tcaggctgaa cgccaagctg
     1081 gtcggctgaa tgaatcgttc atagaaagca tttcgattcg cgcgccatcg tcggcgatct
     1141 gaaatgatca tcgatcttct tgagcgtgcc gccgaactca gtcgggcagg ccagtccttc
     1201 gctctggcga cggtcgtgcg cgccgagagg ccgacctcgg ccaagcccgg cgcaaaagcg
     1261 attgtcaccg tcgacggcgc gctcaccggc tgggtgggtg gcagttgcgc gcagcccacc
     1321 gtggtgcgcg aagcgcttaa agccttgcag gacggcgagc cgcggctggt gcgcctgtgc
     1381 ccgccggaaa aactcggcag cgcgcctcag gaaggcgtcg tcgaagtcgc gctcacatgc
     1441 atcagcggag gcacgctgga ggtgtatatc gagccacact tgccgcgtcc acatctggtc
     1501 gtcgtcggac atctgccgat cgccgaggcc ctgtctcgat tgggcagtgc gctggactac
     1561 gacgtaaccg tgatgggcct ggacgtcacc cccgatcggt ttccggacgc cgatcgcatt
     1621 ctcgatcggt tggacttctc atcgctcaaa ctaaccccgc aggcttacgt cgtcgtcgcc
     1681 acgcatggca attacgacga agacgcgctc gaaggcgcgc tgagatcgga cgcggcgtac
     1741 gtggcactcg tggcgagccg ggcgcgcgcg gaggcgatcc ggcagtacct gcgcgagtcg
     1801 gggttgtccg atgcggcgct ggtgcggctt aaggctccgg ccggccttga tctgggcgcg
     1861 gtgacgcccg aagagattgc gctgagcatc ctggccgaga tcgtccaact tcgacgccct
     1921 ccgacccggc agtcgcacgc cccacctccc gtcgtgcagc cgttggaggc gacggacccg
     1981 gtgtgcggca tgttggtcga cattgccacg gcgcgctaca cgtcacacta cggcagccgg
     2041 gtttactact tttgctcggc cgggtgtcaa cgctcgtttg agaaacaacc ggagaagtac
     2101 gtgacggcga gtgacacgac acccgtcgcc catcacacaa actcctaaag aggaaccacg
     2161 tcatgcggta tgaaggcacg atcacgatta gcgctccacg cggtgtggtg tgggtgtttc
     2221 tcaccgatcc gctccaggtg gcccagtgtg cgccgggcgt cgaatcggtg gaaacactcg
     2281 ttcccgacta caagttccgg gcgatcgccg ccatcggctt cggctcggtg aaggcccgct
     2341 tcactggcga agtggagttc ctggaactcg agccgccgac acgagcgaag ttgaaggcgc
     2401 acggcgacgc gcccggcagc gcagcggacg tcatcagcga gatgttcctg accgacggac
     2461 cggatggaac gaccgaaatg aggtggagcg tcgacatcgt ggtgctgggg acgctggcca
     2521 gcctggcggc gcggctcatg agcagtatca cccagaaact cactgcccag tttttcgagt
     2581 gcctcaaatc caagattgaa gcgtgacgag tgacggacaa ggaggttccc atgcgcggca
     2641 caatgatgga ctacccgttg acgctccagc actttctcga gcgttcacac aagatgtctc
     2701 ccaagaaaga gatcgccagc aaaacggcga ccgggatgca ccgctacacc tacgccgact
     2761 tttacgcgcg tgtgcaccgg ctggcgaacg cgctggccga cctgggcgtg cgcaggggcg
     2821 accgggtggc gacgttcgcc tggaatcact accgccacct ggaactgtat ttcgctatcc
     2881 cttgcatggg ggcggtgctg cacacgctca acattcgcct ctttcccgac caactggtct
     2941 atgtcatcaa ccatgccgaa gataagatca tcttcgtcga cgcaacgctc ctgcctgcct
     3001 tgcaaagggt acaggatcaa atcaagcatg tcgagcgctt cgtgatcatg acggacaccg
     3061 gagaagtgcc ggaaacgact ctgtcgccga cgggcgaata cgagtcgctg ctgagcgcgg
     3121 ccaggcccga gtttcccttc cccaaactcg atgaacttga cgccgccggc atgtgctaca
     3181 cctcgggcac gaccggcaac cccaagggcg tggtctacac ccaccgagcg atctttattc
     3241 actcgttcgc tgaggccatg gccgacagct tggccgtgtc cgagcgtgac acggtcatgc
     3301 cggttgtgcc gatgttccac gtcaatgcct ggggcctgcc cttcaccggc acgatggtcg
     3361 gcgccaagca ggtcttcccc ggcccacacc ttcagccgcg cgacctggcg gagttgatcc
     3421 agaacgagaa agtcaccctg gccgccggcg tccccacgct gtggatcggg ctgtacagcc
     3481 tgctggaacg cgagcagtac gacctgagca gcctgcgcac gctggtcgtc ggcggctcgg
     3541 ccgcgccgcg gacgctgatc gaaaacttcg agaaaaactt cggggtgccg atcacccacg
     3601 cctggggcat gaccgaaatg accccgctgg gcacggtttc caagctcaaa gccgaactgt
     3661 tagatgcacc ggaagaggtg cgcttcgcct accgcgccaa acaagggcgg gccatgccgg
     3721 gcgtggaagt caaggtggtg gacgaggccg ggcaggcggt cgcgtgggat ggcaggacga
     3781 tgggcgaact ttgggtgcgc gggccgtgga tcgccagcag ctactataac gaccccaacc
     3841 tgtcggacgt cttcgccccc gatggctggt tccgcaccgg cgacgtggtg acgcttgacc
     3901 ccgaaggtta catggaaatc accgaccgca ccaaagacct ggtcaagtcg ggtggtgagt
     3961 ggatctccag cgtggcgctg gagagcgccc tgatgggcca tcccaaagtg ctggaggcgg
     4021 ccgtgatcgc tattcctcac gagaaatggc aggagcgccc gatggctgtc gtcgtcccga
     4081 agcccgacca agcggagagc atcaccaaag acgagttgat tgaattcctg cggcccaagt
     4141 tcgccaagtt ctggctgccc gatgacctgg tgttcattaa ggagatgccg aagaccagcg
     4201 ttggcaagtt cgataagaag gttttgcgcg aacgattcaa agagcaccgc acgagcgcgt
     4261 gagccaagaa gaaggagttg ggcctggtgg cctgagcggg taaagatgaa attcggccct
     4321 aatccgttag atcaggcgga aggccagatc ctcggccaca acatcgccgg gctggatggc
     4381 cggcgcctac tgcgcaaggg caagccgctc actcgagacg acgtcgaggt gctgcgccgg
     4441 ttgggacgca cgacagttta tgtcgctgcg ctcgagccgg atgacgtcaa cgaagacact
     4501 gccgcccatc gggtcgccac ggctgcgctc ggcggcaacc tgcggctatc cggctcggcg
     4561 tccggccgcg tgaacttcct ggcgacgcac ctgggcgtgc tgcgcgtgga cgccgatcgg
     4621 ctcgcgcgca ttaacgcctg ccaggatatc acattcgcca cgctggcatc gcactcggcc
     4681 gtgcggcccc ggcagatcgt ggccaccgtc aaggtgattc cttatggggt gccaaattca
     4741 gtcgtgcgcg ccgccgaagc catcgcgtcg gacgatggcc cgctcgtccg cgtcgacgcg
     4801 ctggagccac ggccggtcag cctggtgttc tcaggctctc tgtcgatccg cgacaagctc
     4861 gccgccgact tcgcgccgct gcgccaccgg gtggaagcgc tcggttcgac tattctcagc
     4921 accgactacg tgccgctgga ggacgagaca ggcgaggcag cgctggccga tgccttgagc
     4981 cgccgccggg cggcaggcgc acggcttatc gtcgtcgccg gcgaaacggc gattatggac
     5041 cggcacgaca tcgtgccgcg cgcggtcgag cgggccggcg gatgggtgga atgcctgggc
     5101 gcgccggtcg atcccggcaa cctgctgatg ctggcctacc tcggcgacat gccgatcctc
     5161 ggcgcgccgg gctgcgcccg cagccggaag gtcaatgtcg tagactgggt gctccctcgc
     5221 ctgctcgcgg gcgaccacct gacccgcgac gacatcatcg cgctgggtca tggggggctt
     5281 ctggaggagg cgcctgaacg ccccgccccg cgagagacag tcgaggccgg gggcggtaaa
     5341 tagctgtcag taccgggtga atagccgtca gtccgctctt gactttcgag agaggatgtc
     5401 gtaaattatc cgcgtgacgc agcgctgcgg gggagagcat ggcgtcgtct agccagtcag
     5461 gggaacacat gctgctgctg accgccggga aggccggccg gtggtttgcc gcgtgtatcc
     5521 gcgttcatcc cgttcgcgtc agcaggtaac tccagccgtg cagccatcac gcgctggggt
     5581 tttggttttc gccaccggcc atgccggatt ccatcctaac ctagaaggag ggagacatcg
     5641 tatggaacag agcgtcagta tcaccgtcaa tggggtcaaa cggacccacc cggtcgagcc
     5701 gcgcatgctg ctcgtgcact acctgcggga tgtgctcggc ctgaccggcg cgaacgtcgg
     5761 ctgcgacacg agccagtgcg gctcgtgcac cgtcattctg gacggcatgg ccgtcaagtc
     5821 gtgcactgtg ctggcggtgc aggccgacag gagcagcgtc atgaccatcg agggactggc
     5881 tcaagatggc aaactgcatc cgttgcagca gggtttctgg gaaaagcacg gcctgcagtg
     5941 cggattctgc acgccgggca tgatcatgac cgccgtggat cttctgcaga gaaatcctca
     6001 tccgagcgag gctgaaatcc ggcacgggat cgagggcaac atctgccgct gcaccggcta
     6061 ccagaatatc gtcaaggcga tccaggccgc cgcggaaatg atgggataag gaggcgcacg
     6121 atggctaaat acgttggcgc aagcatcaag cgggttgagg acccgcgctt cattcagggc
     6181 aagggcaact acgttgccaa catcagtttg cccaacatgg cacacgtcgc cattaagcgc
     6241 agcccgtacg gccacgcccg gatcaagaaa ataaacacga agaaggcgac tgcgctgccg
     6301 ggcgtgatcg ccgtgttcac gggccaggac ctgatcgacg gcggcgtcgg caagctgccc
     6361 tgcggctgga acgtgcccga catcaaagtg ccgacgcgct ggccgctgac ggtcgacaag
     6421 gtgcgacacg tcggcgacgg ggtggcgatc gtcgtggccg agaccgcgta catcgcccac
     6481 gacgcgctcg acttgatcga ggtcgattac gagccgctgc cggctgtcgt cgacgcccgg
     6541 aagacgacgg agaaaggcgc gccgctcgtt cacgacgacg tgccgaacaa tatcagttac
     6601 acctgggcca tcggcgacaa ggaggcgatg gagaaggcct tcgccgaggc cgagcacgtc
     6661 gtcacactcg atctggtcaa ccagcggctc atccccaacg ctatggagcc gcgtgcggca
     6721 gtcgcccagt ggagcgccgc gaccgaggag atgacgctgt ggacgaccag ccagaatccc
     6781 cagccgatcc gcttgctgtt gagcgccttc acgctgggca ttcccgaaaa caggttacgc
     6841 gtgatctcgc cggacgtggg cggcggcttc ggcagcaaaa tcttccacta cccggaggag
     6901 atcatcgtcc cgtgggtagc gcgcaagatc aaccggcccg tgaagtgggt ggcgacccgc
     6961 accgaatcca acatgactga ctcacagggc cgcgatcacg tgaccaagtc caggatggcg
     7021 ctgaagaagg acgggacgat caccggcatt cacgttcaaa cgtgggcgaa ccagggcgcg
     7081 tacctttcga cgtttgcgcc gctgatcccg acggcgctgt acctcaccct cctgtctggg
     7141 ttgtacaagg taaagggcat ctggggcgag atgtggggca cactcaccaa cacggtgccg
     7201 gtggatgcct atcgcggcgc cggccgcccc gaagcgactt acgtcatcga gcggctggtc
     7261 gatctcgccg cccgcgacct gggcatggac ccggcggaca tccggcacaa gaacttcatc
     7321 ccgaaggacg agttccctta ccagacgccg gtagctttta tgtatgacag tggcgactac
     7381 gacgcactct tccacaaagc catgacgatg gccgattact cggcgctgcg ccgagaacag
     7441 gcacaggcac gcaagcaggg gcgattggtc ggcatcggca ttgtcgggag catcgaggcc
     7501 agcggcccgg ccccctccaa gatcgccggc gcactcggct cagcggttgg cttctgggag
     7561 agtggcgtca tccgcgtgca cccgtcgggg aaggtcaccg cgctcaccgg atctcacgcg
     7621 cacggccagg gccacgaaac cgcgttcgcg caggtcatcg ccgatgaact gggcgtcagc
     7681 atggacgatg tcgagatcgt ccacggcgac acgggccgcg tgcccttcgg catgggcagc
     7741 tacggcagcc gcagcgcggc ggtcggcggg agcgcgttgg tcaacgccgc caaacgggtg
     7801 cgtcagaaag tgatgaagat ctccgcgcac caactcgaag ccgccgaaga ggacatggtc
     7861 tacgaccagg agagcgggaa gatttacgtc cacggctcgc cgggcaaggc caaatcattc
     7921 ggcgaggtcg cgctggcggc atacacggcc cacaacctgc ccgaaggaat ggagccgggg
     7981 ttggaggagt cggcgtggta cgacccgccc aacttcacct tccccaacac gtgccacatc
     8041 gtccaggtcg aagtggatcg cgacaccggc gaagtgacga ttcagcgtta cgtggcagtg
     8101 gacgacatgg gcaaggtgat caacccgatg atcgtcgagg gtcaggtcat cggcggcgtc
     8161 gtgcagggcg tcggccaggc gctatgggag cacggcatct acgacgagaa cggccagtta
     8221 attaccggat cgctgatgga atacgccgtg ccgcgcgccg aggccttccc gatgatcgag
     8281 acggggcgca acgagacacc ctcgccgcac aacccgctgg gcgtgaaggg tgcgggcgag
     8341 atgggcaccg ttaccgggac cgtcgccgtc gccaacgccg tgatggacgc gctcgcgccg
     8401 ctgggcgtcc ggcacgtcga tatgccgctt acgccggaga agatctggcg ggcgatgcac
     8461 aagagttaga gttgtgagtt agagttagag tatttccact ctaactcatc accctaactc
     8521 taaccgagga gatcaaccat gtatcctgcc agcttcgatt actatcgtcc caagaccgtc
     8581 gccgaagcgg tcaagctgtt gggccaaaac aagaatgcca gagtgctggc cggcggccac
     8641 agcctgctgc cgcagatgaa gctccgcgtc acggcgccgt ccgcgctggt ggacatcggc
     8701 cgcgtcaagg gcctgtcgga catcaaggcg acgaagacgg cgctgaagat cggcgcgatg
     8761 acgacgcacg caacggttgc cgattcggat gtcgtgaaaa agctctgccc gatcctggct
     8821 gaagctgcgg cgttgatcgg cgacgtgcag gtgcgcaatc gtggcacgct cggcggatct
     8881 ctggcgcacg ccgatccttc cgccgactat ccgacagtca tcgtggcgct cggcgcgacg
     8941 attactgtca ccggccccaa aggcagccgc gacatagcgg ctgacaagtt cttcaaggat
     9001 ctgttcacca ccgcgctcag gccggctgaa ctgatcacct ccgtgagcgt tcccgcctac
     9061 ggcggcatgc aaggcatggg cggcgcgtat ctgacgcacg agcatccggc atcgagctac
     9121 gctgtcgttg gcgtggcggc gctcgtcggc ctgaagaacg gcgcatgcgc ccgcgtcagt
     9181 ctggtcgtcg gcggcgcaac ggtgaacccg gtccacgcgg ccgccgccga gaatgcgctg
     9241 acggggcagg cgccgagcga ggccagtatc gccgcagccg cagacaaagt cgccgaagcg
     9301 atagccgatc cgctgagcga cacttatgcc tccggagagt atcgcaagca cctggcaacg
     9361 gtgctcgcca ggcgcgcgct gaccatggct gcggagcggg cgcgagggtg atagactgtt
     9421 ggaacctact tctacaacgt gagtagaagg cggaggcctc cgataacgaa tgtcggaggc
     9481 ctccagcgtg ttatgattgc tcgccataca ggagaatgcc ggatgtaagg atacggctgc
     9541 gtagcggttc aggcaaacgt tccagcaaga caagatcaac ggtgtaatgc gtctgactcg
     9601 acaaccgggc gtgagcaaac acaagtttgc cgcgcgggag tccctcaacg gcgagatcga
     9661 gatcggagtc ggggcgcatt tccggtcccc aggcaaacgc gccaaagaga tagacacgtt
     9721 tggcgccaaa ttcggcggcg agaagctggc tgaggcgctg tgcttcgtgg cgagcatcac
     9781 gagcagcggc ctgcatctca ggtgtaggct cgatccggct gcgctgacgc cagaaggcgc
     9841 gatattgatc taggtccact tgtgccatgt cacctattat aacccagtca gtgtgcacgc
     9901 aacacatgat actgttggcg aattgccttt tggcctatga acgcgtgctg gccgaacaag
     9961 cgagcgggtg caatgcccga tttcgccaac agtatcaatc tttgcatgca cacgtcagca
    10021 cgtcagcaca cgcaggttga cgccaggtta cggccagggt agaatagcat gcgcgtgttg
    10081 gatggagtca tgctccgcga ggtcaaagat gactgttcag gcaatcaccc taactgtgcc
    10141 agagcaagtc tacgaacaga ttcggcgggc agcggaaaag gcgcatcgcc cagtcgacga
    10201 agtcctagcc gaagcggttg cggctgtcgc gccggttatg gacactgcgc cagagagact
    10261 gcgctcggca ctggcgcaca tggcttatct caacgacgcc gctctgtggc aggcggctcg
    10321 cgcgacgatg acagcgaagc agcgagagcg tcttgcagat ttacacgatc agcagcaacg
    10381 cgcaacgctg acgcccgagg agcaggctga ggaacaggcc ttgctggcgc tctaccgcga
    10441 aacgctcctg gtccgggcac aggcagccgt gcttttgaaa cagcgcggct acaacgtatc
    10501 cgacctcgat caatttacgc cactcgaatg agttcttcgt acatttcggt ggcgcttcgc
    10561 agcgagtcgc cgaaacggcg cgctttcgat gcggctattg tctcgcctcg caacgcatca
    10621 tcggcccgct cctcgaactt gatcacattg tcccagaggc acatggcggc acgtcggacg
    10681 aagaaaatct gtttctggcg tgccccatgt gcaacagtca caaggcagat cggcgggaag
    10741 cggttgatcc cgaaagttat gaaccatcca gatgttgtgg ctgcacgccg cctgtgggtc
    10801 atcgctggct ggcatccccc tactgattga cgccctttac ccggcgctcc attaggacgg
    10861 tgaacttgat cattgactcc attgacgacc tgcaaaacgc tctgcgcgag cagctctaca
    10921 tcgccgaccg tgggttggcg acctccatct atcttgcgct caaattgaat cgcccgctgt
    10981 tcctcgaggg cgaggcgggc gtgggcaaga ccgaggtggc gaaggtgctc acacgcctgc
    11041 tcgacacgga cctgattcgc ctgcaatgct acgaaggtct cgacgtgcat cacgccgtgt
    11101 acgagtggaa ctacacgcgc cagatgatgc acattcgcct gctggaggcg cgtggcgaac
    11161 gcccgagcga agcggagctt ttcgggccgg agttcctact gcgccgcccg ctgctgcagg
    11221 cgatcgagaa tgcgcgagag aagtcacccg tgctgctggt cgacgagatc gatcgcagcg
    11281 acgaagagtt cgaggccttc ctgctcgaag tgctgtcgga ctttcagatc tccgtccccg
    11341 agatcggcac gatcaaggcc gagcgcccgc cggtcgtcgt aatcacctcg aaccgcacgc
    11401 gcgaagttca cgacgcgctc aagcgccgct gcctgtacta ctggatcgac tatccctcct
    11461 tcgagaagga gctggagatc gtgcaagcca aggtgccgca tgcctccgcc cagctcgcac
    11521 ggcaggtcac ccggttcgtg caggagctgc gccggatgga tctgtacaag attccgggcg
    11581 tcgccgagac gctcgactgg acggcggcgc tcgtcgcgct cgaccaaagg gcgctcagcc
    11641 catcgatcgt cgacgacacg ctgggcgcgc tgctgaaata ccaggacgac gtcgggaagg
    11701 tcaagggcga tcaggctcgc ctcatcctcg ataggctcgg cgcgttatga cctctccgca
    11761 ggagcctgat ccggactcgg gccacctcct ccacaacctt ctgctcttcg gccggctgct
    11821 gcgcggactg gggcttgacg tcaatccggg ccgcatgatc gacctcacgc atagcctggc
    11881 atacatcgac ctgagccacc ggcaggattt ctaccacgcg gcgcgcagcc tgctcgtcca
    11941 ccggcatgag gacattccgc tgttcgacgc ggcgttcgag gctttctggc gcaagccgga
    12001 agaaggctgg gtgacggtcg atctgcctgg gctgagccgg gcgctgcgtc gccagcggcc
    12061 ggttatcatg ccgccgcctc tgcgaggccc agacagcgcc ggacgacagg ataaggacga
    12121 cacgcctgcc gagaaccggc cgccggtcct tcaggtcacg atgacctaca gcgcgcgcga
    12181 ggtgcttcgc aacaaggact tcgcaagcct gacgggcgag gaattggacg cgatcaagca
    12241 gatgatggcc gcactggtgt ggcagcttgg cgagcggcgc acgcggcggc agcaacccgg
    12301 acgcggccgc ttgctcgacc tgaggcgcac catcaggcgc agcctgaagt acgggggcga
    12361 ggtgatggaa tgggcgcgcc gcgagccgaa gcacaagccg cgcccgctgg tgatcctcgc
    12421 cgacgtcagt ggatcgatgg aacggtacat acgcctcctg ctgcacttcg tatacggggt
    12481 gacggcagga ctcgatcagc ctgtcgaagc cttcctcttc agcacgcgac tgactcgcat
    12541 cacgcgcgag ctgcaggggc gcgacgtcga cgaggcgctg cgcgatgtgt cgcgcaccgt
    12601 gcaggattgg tcgggcggaa cacggatcgg cgaggcgctc aagacgttca atttcaagtg
    12661 gggacgccgg gtgctggggc gaggcgccgt cgtgcttctg atcagcgacg gctgggaccg
    12721 cggcgacatc gagctgctgc gcctcgaaat ggcgcgcctg cagcgcagcg tgtaccgcct
    12781 gatctggctc aacccgctgc tcggctcgcc ggaatacgaa ccgttgacgc gcggcatgca
    12841 ggccgcccta ccctacgtcg acgacttcct gcccgtgcac agcctcgcca gcctcgaaga
    12901 cctcgccaaa cgtctgatcg ggctgaacga gcgtcggcct gaacggaggc agtttagagt
    12961 cttgagttag agttagagtt agagttagag ttagagtggg tcgcacccaa ccataactcc
    13021 tcactctaac tccccactct aactctcact ccaggagggg actatgttca agttcgagag
    13081 aatgatcgcc tggcagaaag ctgtcgaatt cgcggatcat gccttcgacg tggccgatcg
    13141 attgccgcaa cgacaacagt tctcgttcgg cgagcagttg cgtcgggccg cgcttggcat
    13201 cacgaacaac cttgccgagg gcagcggtcg aaggacacca ggcactcgcc gcacatttta
    13261 cgattctggc aaaggctcca cctacgaagt agtcagcatc atagccatcc tgcttcgccg
    13321 aaaggaagtc acgcaaaccg aattcgacac gttctaccgt atgggcgacg agctggcctc
    13381 catcatctcc ggcctcatcg acgccacctt cagagaagaa gccgaattgg gccagacccc
    13441 gggcaggttg cgagacgaag aagaacaata cttgacgagc gaagacaccg actctgaact
    13501 ctaactctcc actctaactc taactcagtg agccatacta tgcgtgaagt cctctccgat
    13561 atcgatcgct ggcaggcaca aggcaaatcg atcgcgctgg caaccgtcgt tcaaacgtgg
    13621 ggttctgcgc cgcgcggtgc cggggcgaag atggcgctga ctctcgacgg cgagatctcc
    13681 ggatcggtca gcggcggatg cgtcgagggt gcggtattcg aatcgggcac gcaagtcctg
    13741 aagaccggcc ggccacagtt gctgagtttc ggcgtggccg acgagacggc gtgggaggtc
    13801 gggctggcat gcggcggcag cattcgcgtg ttcgtcaagc cgctcgattc cgccatgtac
    13861 gacgaagctc acaaggccgt gaccgccgat cgcctggcgg tggtcgtgac cgtcgtgaaa
    13921 gggccggacg aggcgttggg acgcgagctg atgcttcggg aggatggaag ccttgtcggt
    13981 tcactggacg ccggactgga cgaggctgcc atcgcggctg cccgtagcgc gatggaggct
    14041 ggacaatctc agccggcgac gctttcacgc accgacggcg atccgctcga aatcttcgtc
    14101 gacgtgctgc tgccttcgcc gacgctcgtc gccgtcgggg gcgtgcacat cgccatcgcg
    14161 ctggcgtcca tcgccaaggc gctcggctac cggacggtcg tcgtcgatcc gcgccgcgca
    14221 ttcgggagcg agacgcgctt cccgcacgtc gatcagttga tccaggaatg gccggacgac
    14281 gccctggcac agattggatt gaaccgctcg acagcggtag cgatgctgac gcacgatccc
    14341 aagctcgacg acccggcgct gaagatggcg ctgcccagcc cggcgttcta cgtcggcgcg
    14401 ctggggagca acaagacgca ggcagcgcgc cgcgagcgac tgctcgcagc cgggctgacc
    14461 gaggcgcagg tcgatcgact gcacgggccg atcgggcttg acatcggcgc caagacgcct
    14521 gaggagatcg cgctggcggt gatggcgcag atcgtcgccg cgcgcaacgg cgctgcttcg
    14581 tgatgcggtt ggcgccacaa ccttataccg gcagcgcata tccattgcca atccatggag
    14641 ggcgtgctag aataggaccc gacgaaggct ggcaagcggg agtagcgcag gggtagcgca
    14701 agtgcttccc aagcactggg tcgcgggttc gaatcccgtc tcccgctctg taatgaagag
    14761 cgcccttgat gtgcagggcg ctttttcatt ctgggcacgt cgcgtaacaa tgcaagggat
    14821 tggtaattgg tcattggtaa ttggtcatct ggactttgtc caatttggcc ttttcggccc
    14881 gttttaggcg gtttctgcga catgtcgccc cagatatggg ggcacaatgt cgtaatgaat
    14941 gcctatatgt ggcgtcaata tgaaaatgga caa
//
LOCUS       DBNHXB010000151         2794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3723944, whole genome shotgun sequence.
ACCESSION   DBNHXB010000151 DBNHXB010000000
VERSION     DBNHXB010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3723944"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="AC162G_05865"
     CDS             complement(<1..474)
                     /locus_tag="AC162G_05865"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR5075372.1"
                     /translation="MLRYRRTSQLFLSVVLLLSLAGVASASGGGVTFQDVAAGGGAGI
                     TYRRVESPSDDLFDALKRQPVYTLNDLAVTPFNSRGAPGVALLDFDRDGDLDMYVTNG
                     PGAHNSLYSNQLQEAGQVTFVDVGAVAGVGAFDQDSTGVCYGDIDNDGDEDLYVLG"
     gene            complement(485..1816)
                     /locus_tag="AC162G_05870"
     CDS             complement(485..1816)
                     /locus_tag="AC162G_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075373.1"
                     /translation="MVPSPPLGANVAERFQLMLAMTLAGFEGSLRRHMPASAYRDFYL
                     WAISAENPRQDAFLQAMGIPLLTKLTVTLLDNLVDDRAWRQLAECSAPISSYLTYEVV
                     SDNLAIGLASPRPDDMTGVLRRFLVRAFNHAMIARLSGDPRPAAQLLAPLQSTARRIS
                     GFDQSLNRDKQRACSEAYLILRSDLELADLEYAVWPALVANVEACLDLAESLRARHAA
                     GLLREGLARRYQAVNHLLEARDTPLPQLVAVGADAILVAPTLAYYVAVLAEIIQPLER
                     FSHVIADGTLASALYDAALLVRLLNDLGTGLVTLAARERSALLDMMMTQYRECSTART
                     TADLLLNMSDDMDLFTRLRKDILFGEFNVALDGLAHIRLTPDTLLAFGRNLSYLSQLY
                     AQRRTTLQETLAAIGERLGDERVSALISRFVQFHEQLYSNSFHTPAGEYAI"
     gene            2342..2683
                     /locus_tag="AC162G_05875"
     CDS             2342..2683
                     /locus_tag="AC162G_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013334.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HIR5075374.1"
                     /translation="MNDLIERARAMIDAAVAHSRANGGGAIAALHLTIYPTPDVSAET
                     ICEAIDAARRGTPALDARLILTEAPTRYICWNCCGLRFEGKDGICPNCEGEALIVPEE
                     IAFALERVELG"
BASE COUNT          564 a          811 c          881 g          538 t
ORIGIN      
        1 acccagcacg tacaggtcct cgtcgccatc gttgtcgatg tcgccgtagc acacgccggt
       61 gctatcctgg tcgaatgcgc cgaccccggc caccgcaccg acgtccacaa acgtgacctg
      121 gcccgcctcc tgcaactgat tcgagtacag gctgttgtgc gcgcccggcc cgttggttac
      181 gtacatatcg aggtcgccat ctctgtcgaa atcgagaagc gcgactcccg gagcaccgcg
      241 cgagttgaac ggcgtgacag ccaggtcgtt aagcgtatat accggctgcc gcttgagcgc
      301 gtcgaagaga tcgtcgctgg gagactcgac gcggcggtac gtgatgcccg cgccgccgcc
      361 cgctgcgacg tcctgaaagg tgacacctcc gccgctggcg gaagccacgc ccgccaggga
      421 tagcaggagg acaacactca gaaagagttg agacgttctg cgatagcgca acatgatgag
      481 cctcctaaat agcatattcg ccggcagggg tgtgaaatga gttgctatat agctgctcgt
      541 gaaactgaac gaaacgagag atcagtgcgc tcacccgttc gtcgcccagg cgttcgccga
      601 tcgcggccag ggtctcctgg agggtggtac ggcgctgtgc gtatagttgc gacaaatagg
      661 acaggttgcg gccgaacgcc agcagcgtgt ctggcgtgag tcgaatgtgg gccaggccat
      721 ccagggccac gttgaactcg ccgaacagga tgtctttgcg caggcgggtg aagaggtcca
      781 tgtcgtcgct catgttgagc agcaggtccg ccgttgtgcg tgccgtggag cattcccggt
      841 actgcgtcat catcatgtcc agaagcgcgc tgcgttctcg agccgccagc gtcaccagcc
      901 cggtccccag gtcgttgagc agccggacga gcagcgcggc gtcgtacagc gccgaggcca
      961 gtgtgccgtc cgcgatcacg tgagagaagc gctccagcgg ttgaatgatt tcggccagca
     1021 cggcgacgta gtaagccagg gtcggcgcga cgagtatcgc atcggcgccg acggcgacca
     1081 gttgcggcag cggggtgtca cgcgcttcca gtaaatggtt gacggcctgg taacggcggg
     1141 ccagtccttc gcgcagcaaa ccggccgcgt ggcgggcgcg cagcgactcg gccaggtcaa
     1201 ggcaggcttc gacattcgcg accagcgcag gccacacggc gtattccaga tcggccagct
     1261 ccagatcgct gcgcaggatc aggtaggcct ccgagcatgc ccgctgcttg tcccggttca
     1321 agctctggtc gaaacccgag atgcgccgcg cagtggattg cagcggcgcg agcaattgtg
     1381 ccgccggccg gggatcgccg ctcagccgcg cgatcatggc gtgattgaaa gcgcgcacga
     1441 gaaagcgtcg cagcacgccc gtcatatcgt ctggccgtgg actggccagg ccgatcgcca
     1501 ggttatctga cacaacctcg taggtcagat atgagcttat tggtgcgctg cactccgcga
     1561 gctgccgcca ggcccggtca tccaccaaat tatcgagcag agtcaccgtc agcttggtga
     1621 gcagaggaat ccccatggcc tgcaagaagg catcctgcct cggattttct gcagagatgg
     1681 cccataggta gaagtcgcga taggcgctcg ccggcatgtg acgccgcagg ctgccttcaa
     1741 aaccggccag cgtcatcgcc aacatcagtt ggaatcgttc cgctacgttc gccccaagtg
     1801 gtggactcgg aaccatggtg acctcttcag tttacttgcg gcggagcaat cctggccgtc
     1861 ggcctaattc aaccttgccc aaatatgcta attgagaatc gagttggtgt aagatgagat
     1921 tgagtttgac tagagtttgg attccaaata gggtcttttg aggcagttct gcccgatttc
     1981 tcaaccattg cgactccttt tgggatgctg acgcacgaga acaataggaa atccctgatc
     2041 gcaacccgta gcacgcctgc gccgacgggg ttgtgagcag gtctgacctt gtcaagctaa
     2101 cttgatgcaa ctatatgttg aataccggct ggcgtggggt gaaactgaga ttggatagag
     2161 tttgcgttgg gatggcaaaa gagtgtgtta ggtttgtatt gcatttgttt tctggtgaat
     2221 gcccgccaac tttaacgcaa cactcaccca ctctcattga agatgcaacg caaaaccggc
     2281 gaatttcagt gtatagttgc gttacactgc gggcgatcgc ctggcaggat ggagaggtta
     2341 gatgaacgat ctgattgagc gagcgcgggc gatgatcgat gccgccgtcg cacacagccg
     2401 cgcgaatggc ggcggcgcta tcgccgcgct gcacctgacg atttatccca cacccgacgt
     2461 gtccgccgaa acgatctgcg aggcgatcga cgccgcccgg cgcggcacac cggccctgga
     2521 cgcccgcctg atcctgacgg aagcgcccac gcgctacatc tgttggaact gctgcggcct
     2581 gcggttcgag gggaaagatg gcatttgccc gaactgcgag ggcgaggcgt taatcgtgcc
     2641 ggaggaaatc gcgtttgcgc tggagcgcgt cgaactgggc tgagtgacgg gtaaggactt
     2701 cggaagtctc agggcatgac cttgcaggcg aaggcgtctg tcgatcgagg cagtcccgca
     2761 ggcgaggcta tccttaaaga cttccgaagt ctac
//
LOCUS       DBNHXB010000152         4630 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4227445, whole genome shotgun sequence.
ACCESSION   DBNHXB010000152 DBNHXB010000000
VERSION     DBNHXB010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4227445"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="AC162G_05880"
     CDS             complement(<1..202)
                     /locus_tag="AC162G_05880"
                     /inference="COORDINATES: protein motif:HMM:NF047084.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5075375.1"
                     /translation="MLITNATLITWGDNNQILPDHALKVDGDRIAGIGPSAELRAKYP
                     EAEVLDAHGQYVMPGNICAHTHF"
     gene            complement(207..1187)
                     /locus_tag="AC162G_05885"
     CDS             complement(207..1187)
                     /locus_tag="AC162G_05885"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005543 - phospholipid binding [Evidence
                     IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006593 - L-ornithine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR5075376.1"
                     /translation="MQTNLHGRDLIGDLDFSQEEVETVLDVAFDLKRKRALNEPHPYL
                     RDKVLAMLFFFSSTRTRGSFEAGMAQLGGHAAFIESKTTQIAHGDTPKEIGEIFGRYF
                     DGIAIRHVDWRVGNGYLNAVARHSRAPVLNMQCDIYHPFQCLADLMTIMEKKGRDLRG
                     KRMVVSWAYAASYQKPMSVPQSLILQMPRFGMDVVLAHPPEFRLMPDIMGMAKEQAKK
                     FGTGFEVVDDMDAAFKDADIIYAKSWGAMVHTPDADEGAKIIKKYESWITDERRMALA
                     KPNAIYMHPLPADRNIEVTDGVMDGPQSVVYDEAENRLHVQKAVMALTMA"
     gene            complement(1209..4169)
                     /locus_tag="AC162G_05890"
     CDS             complement(1209..4169)
                     /locus_tag="AC162G_05890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5075377.1"
                     /translation="MFIDLILNGKSTQLDCAPGETLLAALRRANVWSVKHGCETGECG
                     ACSVLLDGKIVPTCVLLAAQAEGGNVVTVESLASGHTERVHPIQQAFIDAGAVQCGYC
                     TPAMILATKALLEKEKNPTEAQAREALSAALCRCTGYKKPVEAVLRAAALLRGEAVPP
                     VKGGGIPFESVFGFPRELPPETPETPAEHGGTITKPLVAFPVTITQPQLQTDVVGHPE
                     PKVDAVKLVKGRPVFTDDIELPGMLHAALLTSPHAHARIRNIDATQARALPGVHAVLT
                     YQDVPRVIYASGGQSYPNPPPWDQVSLDSKVRHVGDRVAVVAAETPEIARQAVKLIEV
                     DYEVLPAVFDTEDALRGDVVIHDEPDAVKIHDAKRNVAITNHAEHGDIEKALAESDHV
                     FERTYHVHQVQQASIEPHVVVTYWDEDDRLVIRTSTQVPFHVRRMIAPLLDLPVKRIR
                     VVKPRIGGGFGGKQEMLIEDLCAHLTLATGRPVRFEYTRELEFTSARSRHPQKITYRA
                     GVMKGGALHALDMHVLEDTGAYGTHALTVGTVSGLRGLSTYRCPNLRFVLEAVYTNKP
                     TPGAFRGYGAPQALFALECLMEEIALELNLDPVEFRLKNAVRVGDEVVIARALGEGKE
                     GFEQIIQSCALAECVEQGSAAIGWDRQADPRWRFDPERPHIRRGLGMAACMHGSAIAG
                     LDMGAASIKMNDDGSFNLLVGATDIGTGSDTVLAQIAAETLGCPLSDILPYSSDTDFT
                     PFDTGAYASSTTYISGGAVMRAAARVREQILERAKLMLSVETVEGMYLRDRRVFAPDG
                     RSVSLAEVALHSLHTFDQQQIMATASHMSYHSPPPFGAQFAEVEVDTETGQVTVKKLV
                     MAVDCGTAINPQTAAGQIEGGLAQALGYTVSEEMVYDDAGRLLTTRFGDYRIFQADEM
                     PELEAILVPTYEPSGPFGAKAVAEIPMDGVAPAIANAVHHAVGVRIRDLPLTPEKVWR
                     ALQLTNYQLPVAGH"
     gene            complement(4274..>4630)
                     /locus_tag="AC162G_05895"
     CDS             complement(4274..>4630)
                     /locus_tag="AC162G_05895"
                     /inference="COORDINATES: protein motif:HMM:NF015415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR5075378.1"
                     /translation="AEGELILEVKFSAQTGTGGALARVARTPRDEAIVCAAAVVTKDA
                     ARVAVAGASPQPIVRSSPITNNQLTNNQLQDLVDTIISATHPIGDYRASAEYRKAMAG
                     VLARRALAEAWKRVST"
BASE COUNT          746 a         1475 c         1511 g          898 t
ORIGIN      
        1 agaagtgcgt atgcgcgcag atgtttccgg gcatcacgta ttggccgtgc gcgtccaaca
       61 cctcggcttc gggatacttc gcccgcagtt cggcggacgg gccgatgccg gcgatgcggt
      121 cgccgtccac tttcaaggcg tgatcgggca ggatttgatt attgtcgccc caggtgatga
      181 gggttgcgtt ggtgatcagc atgtcgctac gccatagtga gggccatcac cgccttctgg
      241 acgtgcaggc ggttttcggc ttcgtcgtac accaccgact gcgggccatc catcacgcca
      301 tcggtcactt cgatgttgcg atcggcgggc agtgggtgca tgtagatcgc gttgggtttg
      361 gccagggcca tgcggcgctc gtcggtgatc cagctctcgt acttcttgat gatcttcgcg
      421 ccttcgtccg catccggcgt gtggaccatc gcgccccacg acttggcgta gatgatgtcc
      481 gcatctttga atgcagcatc catgtcatcc accacttcga agcccgttcc gaatttcttc
      541 gcctgctctt tggccatgcc catgatgtcg ggcatgagcc tgaactcggg cgggtgcgcc
      601 agcacgacat ccatgccgaa gcgcggcatc tgcaaaatga gcgactgcgg aaccgacatg
      661 ggcttctgat acgaagccgc gtatgcccac gacacaacca ttctcttgcc gcgcagatcg
      721 cgccccttct tttccatgat cgtcatcagg tcggcgaggc attggaacgg gtgatagata
      781 tcgcactgca tgttgagcac gggcgcgcgc gagtgccggg ccacggcgtt gagatagccg
      841 ttgccgacgc gccagtcgac gtggcggatg gcgatgccgt cgaagtagcg gccgaagatt
      901 tcgccgatct ctttgggcgt atcgccgtgc gcgatctgtg tggtcttgct ctcaatgaag
      961 gcggcgtggc cgccgagctg cgccatgccg gcttcgaacg agccgcgcgt gcgggtgctg
     1021 ctgaagaaga acagcatggc cagcaccttg tcgcgcaggt aggggtgcgg ctcgttcagc
     1081 gcgcgcttgc gcttcagatc gaacgccacg tccaggacgg tttcgacttc ttcctggcta
     1141 aagtcgagat cgccgatcag gtctcgaccg tgaaggttgg tttgcatctt ctcgtctcct
     1201 ggtgtgggtc aatgacccgc aacgggtaat tggtaattgg taagttgcag agcgcgccac
     1261 accttctccg gcgtgagcgg caaatcgcgg atgcggactc cgaccgcgtg atgcacggcg
     1321 ttggcgatgg cgggcgcgac gccgtccatc ggaatctcgg ccacggcctt cgccccgaac
     1381 gggccgcttg gctcgtaggt cgggaccagg atcgcttcga gttccggcat ctcgtcggcc
     1441 tgaaagatgc ggtaatcgcc gaagcgcgtg gtgagcaggc ggcctgcgtc gtcgtagacc
     1501 atctcctcgc tcaccgtata gccgagagcc tgggccaacc cgccctcaat ctggccggcg
     1561 gcggtctgcg ggttgatggc cgtgccgcag tccacggcca tcaccagctt cttcactgtc
     1621 acctgtccgg tctcggtgtc cacttcgacc tcggcgaact gcgcgccgaa gggcggcggc
     1681 gagtgatacg acatgtggga agccgtggcc atgatctgct gctggtcgaa ggtgtggagg
     1741 ctgtgcaggg cgacctcggc cagcgacaca ctgcggccat cgggcgcgaa cacgcggcgg
     1801 tcgcgtaggt acatcccctc gacggtctcg acgctcagca tcagtttcgc gcgctcaaga
     1861 atctgctcgc gcaccctggc ggcggcgcgc atcaccgcgc cgccagagat atatgtcgtg
     1921 ctggaggcat acgcgcccgt gtcgaacggc gtgaaatccg tgtccgacga atagggaagg
     1981 atgtccgaga gcggacagcc caacgtctcg gcggcgatct gcgccagcac cgtatccgag
     2041 ccggtgccga tgtcggtcgc gccgaccagc agattgaaac tgccgtcgtc gttcatcttg
     2101 atgctggccg cgcccatgtc cagcccggcg atggccgagc cgtgcatgca ggcagccatg
     2161 cccagcccgc gccggatgtg cggccgctcc gggtcaaagc gccaacgggg gtcggcctgc
     2221 cgatcccacc cgatagccgc gctgccttgc tccacgcact ccgccagagc gcacgactgg
     2281 ataatctgct caaagccttc cttgccttcg cccaaggctc tggcgatgac gacctcatcg
     2341 ccgacgcgga cggcgttctt cagccggaac tccaccgggt cgaggttcag ctccagcgcg
     2401 atctcttcca tcaggcattc gagcgcgaac agcgcttgcg gggcgccgta gccccggaac
     2461 gcgccgggcg taggcttgtt ggtatacacg gcctccaaca cgaagcgcag gttggggcag
     2521 cgataggtgg atagcccgcg caagccgctc accgtgccca ccgtgagcgc gtgcgtgcca
     2581 tacgcgcccg tgtcttccag cacgtgcatg tcgagcgcgt gcagcgcgcc gcctttcatc
     2641 acgcccgcgc gatacgtgat cttctgcggg tggcgcgagc gcgcactggt gaactcaagc
     2701 tcgcgcgtgt actcgaagcg cacgggccgc ccggtcgcca gcgtgagatg cgcgcacagg
     2761 tcttcgatga gcatctcctg cttgccgccg aagccgccgc cgatgcgggg cttgacgacg
     2821 cggatgcgct tgaccggcag atcgagcagg ggggctatca tgcgccgcac gtggaacgga
     2881 acctgagtgc tggtgcgaat caccaggcgg tcgtcttcgt cccagtacgt caccaccacg
     2941 tgcggctcaa tcgaagcctg ctgaacctga tggacgtgat aggtgcgctc aaagacgtga
     3001 tccgactcgg ccagcgcctt ttcgatgtcg ccgtgctcgg catgattggt gatggcgacg
     3061 ttgcgtttcg cgtcgtggat cttcaccgcg tccggctcgt cgtggatcac gacgtcgccg
     3121 cgcagagcgt cctcggtgtc gaagacggcg ggcaggactt cgtaatcgac ctcgattagc
     3181 ttgaccgcct ggcgcgcgat ctccggggtc tccgccgcga ccaccgccac gcggtcgccc
     3241 acgtgccgga ctttcgagtc gagtgagacc tgatcccacg gcggcgggtt gggataagac
     3301 tggccgcccg aggcataaat tacgcgcggc acatcctgat aggtcagcac cgcgtgaacg
     3361 ccgggcaggg cgcgagcctg ggtggcgtct atgttccgaa tgcgggcgtg cgcgtgtggg
     3421 ctggtgagca atgccgcgtg caacatgcca ggcagctcga tgtcgtcggt gaacacgggg
     3481 cggcccttga ccagcttcac cgcgtccact ttcggctccg gatggcccac cacgtcggtc
     3541 tgcagttgag gttgcgtgat agtgacaggg aaagcgacga ggggtttggt gatggttccg
     3601 ccgtgttccg ccggggtttc gggagtctcg ggcggcagct cgcgagggaa gccaaatacg
     3661 gactcgaagg gaataccccc gcctttcaca ggcggcacgg cctcgccgcg cagcagcgcc
     3721 gccgcgcgca gaacggcctc caccggtttc ttgtagccgg tgcagcggca cagcgccgca
     3781 ctcagggctt cgcgggcctg cgcttcggtg gggttcttct ctttttcgag cagcgccttc
     3841 gtcgccagga tcatcgccgg ggtacagtag ccgcactgca ctgcgcccgc gtcgatgaag
     3901 gcttgctgaa tgggatgcac ccgctccgta tgcccgctcg ccagcgactc gacggtgacg
     3961 acgtttccgc cttcggcctg cgccgccagc aggacgcacg tcggcacaat cttgccgtcg
     4021 agcaggaccg agcaagcgcc gcactcgccg gtctcgcagc cgtgcttgac gctccacacg
     4081 tttgcgcgcc gcagcgcggc caggagcgtc tcgcccggcg cgcagtcgag ttgagtggat
     4141 ttgccgttga gtatcaaatc gataaacatg ttggcctctg gggctcaagg aactcaggga
     4201 agtgaaggaa ctcggggaac tgaaggaagc tccggtgttc gtgccttgag ttccttcgct
     4261 cccttcgttt ccctcaggtg ctcacccgtt tccacgcttc tgccagcgcc cttctcgcca
     4321 acacgcctgc catggctttg cggtattcgg cgctggcgcg gtaatcgccg ataggatggg
     4381 tggcggagat gatcgtgtct accaggtcct gtaattggtt attggttaat tgattgttgg
     4441 tgattggact gctgcggacg atgggctgcg gactcgcgcc cgccacggcc acacgggcgg
     4501 cgtctttcgt caccaccgcc gcggcacaga cgatggcctc gtcgcgcggg gtgcgggcga
     4561 cgcgcgccag cgctcctccg gtacccgtct gcgccgagaa cttcacttcg agaatcagct
     4621 cgccctcggc
//
LOCUS       DBNHXB010000153        15969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2382633, whole genome shotgun sequence.
ACCESSION   DBNHXB010000153 DBNHXB010000000
VERSION     DBNHXB010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 15969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15969
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2382633"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /gene="ptsP"
                     /locus_tag="AC162G_05900"
     CDS             <1..1563
                     /gene="ptsP"
                     /locus_tag="AC162G_05900"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR5075379.1"
                     /translation="KTRAQIAGMRESVNRRAGAYSAAIFEAHALYLDDEALREPARRA
                     IFVDRLNAAAAWQRAAEQVAAEYRALDDEYQRARAGDVVDVGRQVVLNFLGEAVSAPV
                     MSEVGILIAPDLTPADTARLDPRRVQGICTAFGGPTSHSAILARSLDIPAVVGLGERI
                     LEIAEGTLLIVVGMTGQVFPDPEASLVAHYAQRAGDARAAKAEARAASAAPAGTRDGR
                     RLEVVANIGSPDEARAAVDAGAEGVGLFRTEFLFLDRQVAPDEEVQYAAYCAAAQGLE
                     GRPLIIRTLDVGGDKPLPYLDLGAEANPFLGWRAIRLCLAKPDLFKAQLRAIVRAAAD
                     FPVKVMFPMVATLAEWRAARALLEEARLEVRNLGQAAPDRIETGIMVEIPSAALQATQ
                     FAEEVDFFSIGTNDLSQYTLAAERGNARVAALADGFQPAVLRLIQLVVEAAHARGKWV
                     GVCGEMGGDPLAVPLLVGLGVDELSMNTPAIPKAKQIIRGLDSSEARKLAQAALELGS
                     AEEVRRAMSDPP"
     gene            complement(1582..2343)
                     /locus_tag="AC162G_05905"
     CDS             complement(1582..2343)
                     /locus_tag="AC162G_05905"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018399521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR5075380.1"
                     /translation="MDIAIAAARQAGALLGMYWERGVVAEYKGAIDLVTEADRASEAL
                     ILDVLSRHFPDHRFHGEETGANGAPSSHVWLIDPLDGTTNFAHGYPQFSVSIALRIDG
                     RTEIGVVFDPLRDELYTAGRGRGASLNGRSIRVSATPDLAHSFLVTGFPYDRQTSGHN
                     NIQEHRAFLMQSQGVLRAGSAALDLAAVACGRLDGYWEYKLSPWDWAAGILLVEEASG
                     RVTDAQGGAVGSRPEGIVASNGRIHEEMLDVLRDA"
     gene            complement(2461..2781)
                     /locus_tag="AC162G_05910"
     CDS             complement(2461..2781)
                     /locus_tag="AC162G_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HIR5075381.1"
                     /translation="MGEFVTVAKTSEIAPGTVKVVRVDGVPIGVANVDGEFYAFADVC
                     THDDGPVAEGELDGCQIECPRHGARFDIRTGAVKLLPAVVPIPVYPLQVIGDEIQVSS
                     VPKS"
     gene            complement(2811..3209)
                     /gene="sufU"
                     /locus_tag="AC162G_05915"
     CDS             complement(2811..3209)
                     /gene="sufU"
                     /locus_tag="AC162G_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIR5075382.1"
                     /translation="MQDLYMENILDHYENPRNKGRLEHPDISHEEDNPVCGDRIRIDL
                     KVEDGVIADARFDGRGCSISQAAASMLIEDIIGKPLDEVKGYDKAHVLDMLGIPIGPV
                     RMKCALLSLKVLKAGAYGIHGWPGEDEEES"
     gene            complement(3248..4504)
                     /locus_tag="AC162G_05920"
     CDS             complement(3248..4504)
                     /locus_tag="AC162G_05920"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HIR5075383.1"
                     /translation="MIPTSAPRTLDVQAVRADFPILSQSVNGKPLAFLDSAASSQKPL
                     AVIEAMNEYYRTTHANVHRGVYTLSERATELYENARKKVARFIGAKSSREIIFTRNTT
                     ESINLVAQAWGRANLRPGDEILLTELEHHSNLVPWQLIARETGARIRYIPVDGQGVLQ
                     LDGLDTLLTERTKIVAFTQMSNMLGTITPAAEIVRRATAAGALTLIDAAQGVPHLPTN
                     VQALGCDFLAFSGHKMCGPTGIGVLYGRRDLLEAMPPFLSGGDMIRRVTFEGAEWNEL
                     PWKFEAGTPAIAEGIGLGAAVDYLSGLGMDAVRAHERETIAYALERLAEVPTVAIYGP
                     MDPDIRGGVATFNLGDIHPHDVAAVLDTEGIAIRAGHHCAMPLHEKFGLTASSRASFY
                     VYTIPEEIDRLVEGLYKARSIYKRTK"
     gene            complement(4527..4916)
                     /locus_tag="AC162G_05925"
     CDS             complement(4527..4916)
                     /locus_tag="AC162G_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIR5075384.1"
                     /translation="MDRQEQIDFILDHYENPRRKGALPDAGASAQGSNPGCGDLVTMY
                     VKVDPRTGRADVTFDGTGCTISQASASYLSEFASGKTLEEVLTVDHDVWMDALGREIA
                     QQRPGCAMLPLDTLREAIGKLDRLQAL"
     gene            complement(4923..6329)
                     /gene="sufD"
                     /locus_tag="AC162G_05930"
     CDS             complement(4923..6329)
                     /gene="sufD"
                     /locus_tag="AC162G_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HIR5075385.1"
                     /translation="MTTLNDETTARAVPLAASEPVREAETGLAPAALGFSRESLDALI
                     ASRPFEPDWMRDVRLDAWRILQDTPLPARNDEAWRRTDYSALKFEEVATLAQDDDLKT
                     ALKHTPMPVRNVLRDKHASGALLQVDGLVGGNWLDEPLRQRGVIFTDMDAALREHGDL
                     LKRYFMTEIVKPNDGYFAALHAAFWQGGAFLYVPAGVDVPMPLRAATWLSEHAASFTH
                     TLIVVERGARVVFIDEYASRTQVRQALVCGAVEMQAGPGAQLDYVNWQDWGRDVFNFT
                     HERAAVERDATLHWVVAGLGGRLTKSFLDSRLVGEGSTALMSGVYFVDGTQHMDYDTE
                     QNHVAGHARSDLLYKGALKDKARAVWQGNIRVYPGAQRTDAYQANRNLALSHEARTDS
                     IPGLEIEANDVRCTHGSAVSQIDREEVFYLMTRGVPKPVAEQLIVNAFFQPVLDRIPH
                     KSVRRRLEKSFAAKMGLA"
     gene            complement(6467..7873)
                     /gene="sufB"
                     /locus_tag="AC162G_05935"
     CDS             complement(6467..7873)
                     /gene="sufB"
                     /locus_tag="AC162G_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR5075386.1"
                     /translation="MATQEQNLQSLKEYKYGFVDPATHVFKSGKGFTREVVEEISAMK
                     GEPEWMRKFRLKAYAHAVQRPTPTWGGDLSGLNLDEIYFYIRPSEKTEASWDEVPETI
                     KNTFDKLGIPEAERKFLAGVGAQYESEMVYHSIQENLEKQGVIFLSSDDGLKHYPDLF
                     REHFGTIIPIQDNKFAAINSAVWSGGSFVYVPKGVHVEMPLQAYFRLNSESVGQFERT
                     LIIVDEGAQVHYVEGCTAPVYSRDSFHSGVIEIVVKKGARCRYTTIQNWSANVYNLVT
                     QRAIVEEDATMEWTDANLGSKLTMKYPSCYLMGKGAKGEILSMAFAGTGQHQDTGGKV
                     VHVAPYTRSKITSKSISMNRGRASYRGLLKVHKGAEGAQSHVQCDALLLHEESRTDTY
                     PYIEIDEDRVTIGHEATVSKIGDEQLFYLMSRGLDEVTARSMVVSGFIEPLVKELPME
                     YAVEMNRLIQLQMEGSVG"
     gene            complement(7891..8724)
                     /gene="sufC"
                     /locus_tag="AC162G_05940"
     CDS             complement(7891..8724)
                     /gene="sufC"
                     /locus_tag="AC162G_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR5075387.1"
                     /translation="MADKLEIRNLLVKIEDKEILKGVTLTVEQGQVHALMGPNGSGKS
                     TLAYVIAGHPGYQVIEGEVVWKGQNVLNLSADERSRLGLFLAFQYPVSIPGVTVANFL
                     RTALNARRKVGNGASGGSSGPLVATQAAGKKGISIPEFRKLLIAKMKMLKMDPAFAGR
                     YLNEGFSGGEKKRNEILQMAVLEPEMAILDETDSGLDIDALKLVAEGVNTLLGPNMGA
                     LVITHYQRLLNYIKPHVVSIMYDGRIVLTGGPELALELEEGGYESIRMRLGLPSVAEP
                     A"
     gene            8900..9529
                     /locus_tag="AC162G_05945"
     CDS             8900..9529
                     /locus_tag="AC162G_05945"
                     /inference="COORDINATES: protein motif:HMM:NF016581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5075388.1"
                     /translation="MQDNRSAVLNLLRERGEATAADIAVALDTTPVNAHYHLSRLERE
                     GLIVSEPLRQGVGRPKYVYSLAGAALQLVPQSTHRLADRLLDALQSQLTAQQIEAIFN
                     HMVEDITAEHGAELGDRSLEEKIETLVEVLGEEGFFARVEKIDQDFQLTQCGCPYRYV
                     AARHPSICAIDLQLINTALGAEVERQSWILNGDNVCTFHVKAPALDGNP"
     gene            9539..9880
                     /locus_tag="AC162G_05950"
     CDS             9539..9880
                     /locus_tag="AC162G_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIR5075389.1"
                     /translation="MSDIAENTTAEAGIQYSEQEIGLRDALRDVVDPELGMSIVELGL
                     IRKIDFHPDLTEITMIMTTPFCPYGPALLEQTRRTAEKHLQGPVKITLGMEMWEPSMM
                     EGGPPAEWGLY"
     gene            9998..10813
                     /locus_tag="AC162G_05955"
     CDS             9998..10813
                     /locus_tag="AC162G_05955"
                     /inference="COORDINATES: protein motif:HMM:NF024762.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HIR5075390.1"
                     /translation="MTTEFATAVSVPDDEIDLARAALLFARDAYPEIDPDAYVRQLDT
                     WAGSIRVAVESRRSDPSFGIVTDLLFDRLGFQGSEPDYSDPRNSYLNQVIDRRRGLPI
                     ALSVIYLEIGWRVGLPLSGVGLPGHFIIRCDSGSRTWFIDPFHQGGVLDEEDCARLMR
                     RTAGDLPFSRSLLLPVTRRQILARMLHNLRATYVQRDMLREAQPVIERLIELDPASGE
                     PVRDLGLIHFRQGAYRRAIDLLESYLGLAPDTDDVASIRQVIGAARAEMARRN"
     gene            10933..12111
                     /locus_tag="AC162G_05960"
     CDS             10933..12111
                     /locus_tag="AC162G_05960"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR5075391.1"
                     /translation="MRTTTFRRMSGVLFVLAAMGLGYVAGLLTPVAGEPAATTGAPSG
                     ALYEEAWQLVDRHFFGTLPPTQTRTYAAIRGMLAALGDQYTVLIEPPAARLESDQLRG
                     EFGGVGADVRRDAAGRTLLSPYPDGPAARAGVVEGDELTAVDGKPVAELPRLDEIEAL
                     LRGDVGSRVRLTLVREGQAFDVEVERANVAPPSTMWRVLAETPDIGYISIRLFTDRTA
                     DEVRQALEDLRGQGARALILDIRDNGGGLLDAAVDVTGQFIDGVVLIERKRDEMERQF
                     TAAADGAARDMPLVVLVNHSTASASEIVAGALRDRQRAILIGEPTYGKGSVQSIYALS
                     DGSSLHITTAEWYTPGHDDFNGQGLRPDIPVARSEDDRAAGRDPALDAAVAHLTQILP
                     "
     gene            12213..13439
                     /locus_tag="AC162G_05965"
     CDS             12213..13439
                     /locus_tag="AC162G_05965"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR5075392.1"
                     /translation="MVIVIVAVGSTMYVVGLNAGRSQPAAPVAAIISPTPDAPSSTPV
                     PGTPTAATGLDDGSIDEELGIFREAYDLIQSEFFGEIPSDNEIVYGAIRGMLQRLDDQ
                     NTTFLEPSIAELDREARTGSYGGIGALVNLSESQALELVRVFKGSPAEQEGMRAGDQV
                     IAVDDRSIVGLSLDEMVALVRGPAGSDVRLTVVRSGVEEPFEVVVTRAQIEIPLVESR
                     MIGSDIAYINLTQFDGTATEQLTQEIESLLARNPNGLILDLRGNPGGFLDEAINVADL
                     FLAEGVVMYERERDGAEDVYRSDDGDLAEEIPLVVLVDGGSASASEIVAGAVQDRDRG
                     TLIGTLTFGKGSVQRIHTLSDSSELRITIARWFTPNDRAIHGEGLEPDIVVEPGEDAA
                     TDPQLDRAIELLRTGQ"
     gene            13473..13961
                     /gene="smpB"
                     /locus_tag="AC162G_05970"
     CDS             13473..13961
                     /gene="smpB"
                     /locus_tag="AC162G_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007391381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR5075393.1"
                     /translation="MAKPKAPLADRSAVKVVATNRAARHDYAIDERFEAGIALLGTEV
                     KSIREGGANLREGYVQVRGGEAFLVNTHIAPYDPAGRTGHDPLRSRKLLLHKKEIARL
                     ETGTRERGWTVIPLRMYLKNGRLKIEIALARGKRQFDKRQAIAKRDAERQIDRSLKER
                     RS"
     gene            14058..14936
                     /locus_tag="AC162G_05975"
     CDS             14058..14936
                     /locus_tag="AC162G_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR5075394.1"
                     /translation="MSLESDGVALFIDFDNVRYSLLNERGKEIEPRELMAKARTYGRV
                     VAAKAYADFSEHPLEFRRSLEVAGIDVRDIPKGGLGKNKSKKSSPEIAMVLDVFELLL
                     DKPTISTLMLMSGDRDLIRLVTMARHRFGKRVVISGVPETVSRDLAAAADALDPLVPP
                     VGLEPEIRRAPEPAALPEEAPDTMDIGEDEIRLVRSIYHHRDRRYRSFNFLAEKLASD
                     RNPNRVGDLDEAKTALEAAVADRMLLPEEIDTDMGYSVTSYWLNEEHPKVKFVLSRMA
                     ENGEQPQNSEGHAIAQ"
     gene            14966..15354
                     /gene="ssrA"
                     /locus_tag="AC162G_05980"
     tmRNA           14966..15354
                     /gene="ssrA"
                     /locus_tag="AC162G_05980"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(15392..15700)
                     /locus_tag="AC162G_05985"
     CDS             complement(15392..15700)
                     /locus_tag="AC162G_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR5075395.1"
                     /translation="MPNFGVTVAVIHDRQVLLTRREDFRVWCLPGGAVESGESVAEAA
                     MREVRQHATWPFAQATTRSQVYALLQQGAVPIEQLQSYVCKLPQPNDELLEVPGDSLR
                     "
     gene            complement(15709..>15969)
                     /locus_tag="AC162G_05990"
     CDS             complement(15709..>15969)
                     /locus_tag="AC162G_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HIR5075396.1"
                     /translation="DQRDLAGQLGKKSAKTPGKTDGVAWQPGPLTGSPIVDSCLGWLE
                     CRVTGSVPSGDHVVYVAEVVEAGVQREGAPLTMAESGFKHSG"
BASE COUNT         2914 a         5188 c         4959 g         2908 t
ORIGIN      
        1 aagacccggg cgcagatcgc ggggatgcgc gagtcggtga accgccgcgc cggcgcatac
       61 tcggctgcga tcttcgaggc gcacgcgctc tacctggacg acgaggcgct gcgtgaaccg
      121 gcgcgacgcg ccatcttcgt cgatcgactc aacgccgccg ccgcctggca gcgcgccgct
      181 gagcaagtcg ccgccgagta tcgcgcgctc gacgacgaat accagcgcgc tcgcgctggt
      241 gatgtggttg acgtgggtcg acaggtcgtg ctcaactttc tgggagaagc tgtttcggcg
      301 cccgtcatgt cggaggtcgg cattctcatc gctcccgatc tgacgcctgc ggacacagcg
      361 cgcctcgacc cgaggcgtgt gcagggcatt tgcactgcct tcggcgggcc gacttcgcac
      421 agcgcaattc tcgcgcgtag tcttgacatt cccgccgtcg tcgggttggg cgagcgcatt
      481 ctcgagatcg cggaaggcac gttgttgatc gtcgtcggta tgaccggcca ggttttcccg
      541 gacccggaag ccagcctcgt cgctcactat gcacagcgcg ccggggacgc ccgcgcggcg
      601 aaagccgaag cgcgcgccgc cagtgcagcg ccggccggta cgcgcgacgg ccgccgcctc
      661 gaggttgtcg ccaacattgg atcgccagac gaagcgcgcg ctgccgtaga cgccggagcg
      721 gagggggtcg ggctgttccg cacggagttc ttgtttctcg atcggcaggt cgcgcctgac
      781 gaagaggtgc aatatgctgc atactgcgcc gccgcacagg ggcttgaagg gcgaccgctg
      841 atcattcgca cactcgacgt aggcggcgac aagcccctac cctacctgga tctcggcgcg
      901 gaagcgaacc cgtttctggg ctggcgcgcg attcgcctgt gcctggcgaa acctgacctg
      961 ttcaaggcgc agctgcgcgc catcgtgcgc gccgccgcag attttccggt gaaagtgatg
     1021 tttccaatgg tcgccacgct cgccgaatgg cgagcggcgc gcgcgctgct ggaagaagcg
     1081 agactcgagg tcaggaatct cggccaggct gcgccggacc ggatcgaaac cggcatcatg
     1141 gtcgagatcc cctccgcagc gctgcaggcg acacagttcg ccgaggaagt tgatttcttc
     1201 tcgatcggca cgaacgacct gtctcagtac acgcttgccg ccgagcgcgg caacgcgcgc
     1261 gtcgcggcgc tcgcggatgg atttcagcca gccgttttgc ggttgattca gttggtcgtg
     1321 gaagccgcgc acgcgcgggg caagtgggtc ggcgtgtgcg gcgagatggg cggcgatccg
     1381 ctggccgtgc cgctgctcgt cgggctgggc gtcgacgagc tgagcatgaa cacgccggct
     1441 atcccgaagg ccaagcagat cattcgcgga ctggattcga gtgaggcgcg caaactggct
     1501 caggccgcgc tcgagctggg gtcggcggag gaggtgagac gggcgatgag tgacccgccc
     1561 tgacacgtga atgtcctccc gtcacgcatc ccgcaacacg tccagcattt cctcatgaat
     1621 cctgccgttg cttgcgacga tcccttccgg ccgcgagcct accgccccac cctgcgcatc
     1681 tgtcactcgg ccgctggctt cttccaccag cagaataccc gccgcccagt cccatgggct
     1741 gagcttgtac tcccagtagc catcgagccg gccgcacgcg acagcggcca ggtcgagcgc
     1801 cgcgctgccc gcgcgcaata cgccctgcga ttgcatcagg aatgctcgat gttcctgaat
     1861 gttattgtgc ccgctcgtct gccgatcgta cggaaaaccg gtcaccaaga agctgtgcgc
     1921 cagatcggga gtcgccgaga cccgaatcga ccgcccgttg agggacgcgc cgcgcccgcg
     1981 tccggcggtg tatagctcgt cgcgcagcgg atcgaatacg acgccgattt ccgtccgccc
     2041 atcgattcgc agcgcgatcg acacgctgaa ctgcggatag ccgtgtgcga aattcgtcgt
     2101 gccgtccagt ggatcgatca gccacacgtg cgacgaaggc gcgccgttcg caccggtctc
     2161 ttcgccgtgg aaacggtgat ccgggaagtg tcgagacagc acatccagaa tgagcgcctc
     2221 ggacgcgcgg tccgcctcgg ttaccaggtc gatcgcgccc ttatactcgg cgacaacgcc
     2281 gcgctcccaa tacatgccca gcagcgcgcc ggcttgtcgg gccgccgcga tagcgatgtc
     2341 catcatctcc atacatgctt gctccgtgat aaattaagaa aaaaggccag ccagtaccag
     2401 gccagcctca aaggacgact gaagtcatca ctacgacatc gtgccttcgg aagtctcaat
     2461 tcaactcttg ggaacagaac tgacttgaat ctcgtcgccg atcacctgca gcggatacac
     2521 cggaatcggc acgaccgccg gaagcagctt caccgcaccg gtgcgaatat cgaaccgcgc
     2581 gccatggcgc gggcactcga tttggcaacc gtccagctcg ccctcagcca ccgggccgtc
     2641 gtcgtgcgtg cacacgtcgg cgaacgcgta gaactcgcca tcgacgttgg ccacgccgat
     2701 cggcacgccg tcgacgcgca cgaccttcac cgtccctggc gcaatctcac tcgtcttcgc
     2761 aaccgtcaca aactcaccca tcgcctaact cgccactcta actccccact ctaactctct
     2821 tcctcatctt cccccggcca gccatgaatg ccgtatgcgc ctgctttcag caccttcagc
     2881 gacagcagcg cgcatttcat gcgcaccggc ccgatgggga tacccagcat gtccaggacg
     2941 tgcgccttgt cgtagccttt cacttcgtcc agcggcttgc cgatgatgtc ttcgatcagc
     3001 atcgacgccg ccgcctggct gatcgagcag ccgcgtccgt cgaagcgcgc gtcggcgatg
     3061 acgccatctt ccactttcaa gtcgatacga atgcgatcgc cgcagaccgg attgtcttcc
     3121 tcgtgcgaga tgtccggatg ctcgagccgc cccttgttgc ggggattctc gtagtgatcg
     3181 aggatgttct ccatgtagag gtcttgcatc gaccgcctcg aagtgaccgg cacctttcag
     3241 gtgccggtca cttggttctc ttgtaaatcg acctggcctt gtacaacccc tcgacgagcc
     3301 gatcgatttc ttcgggaatc gtatagacgt agaacgatgc gcgggacgac gccgtcagcc
     3361 cgaacttctc gtgcagcggc atcgcgcagt gatgaccggc gcgaatggcg atgccctccg
     3421 tatcgagcac ggcggccacg tcgtgcggat ggatgtcgcc tagattgaac gtcgccacgc
     3481 cgccgcgaat gtccggatcc atagggccat aaatggcgac ggtcggcacc tcggccagcc
     3541 gttcgagggc ataagcgata gtctcgcgct cgtgcgcgcg cacggcgtcc atccccaggc
     3601 ccgaaaggta atcgacggcc gcgcccagcc caatgccttc ggcgatcgcg ggagtgcccg
     3661 cctcgaattt ccagggcagc tcgttccatt ccgcgccctc gaacgtcaca cggcggatca
     3721 tgtcgccgcc gctgaggaag gggggcatcg cctccagcag gtcgcgccgg ccgtacagaa
     3781 cgccaatgcc ggtcggcccg cacatcttgt ggccggagaa cgccaggaaa tcgcagccca
     3841 atgcctgcac gtttgtcggc agatgtggca cgccctgcgc ggcgtcgatg agcgtcagcg
     3901 cgccggctgc cgtcgcgcgg cgcacgatct ccgccgccgg cgtgatcgtg ccgagcatgt
     3961 tgctcatctg cgtgaaggcc acgatcttcg tgcgttccgt cagcagcgtg tccagcccgt
     4021 cgagctgcag aaccccctgc ccgtccaccg gaatgtagcg gatcctcgcg ccggtctctc
     4081 gcgcgatgag ctgccagggc acgaggttgc tgtggtgctc cagttcggtc aataaaatct
     4141 cgtcgcccgg cctgaggttc gccctgcccc acgcctgcgc gaccagattg atcgactccg
     4201 tggtattgcg ggtaaagatg atttcgcggc tcgacttcgc gccgatgaac cgggccactt
     4261 tcttgcgcgc gttctcgtac agctccgtcg cacgctcgct tagcgtgtac acgccgcgat
     4321 gcacgttggc gtgcgtcgtg cggtagtact cgttcatggc ctcgatgacg gccagcggct
     4381 tttgggacga ggcggcgcta tccagaaacg ccagcggctt gccgttgacg ctctggctca
     4441 agatgggaaa gtcggcccgg acggcctgca cgtcgagcgt gcgaggcgcg gacgtgggta
     4501 tcaagatcgt cactcctgtc tagtctctaa agcgcctgta gccggtcgag cttgccgatc
     4561 gcctctcgca gcgtatccag cggcagcatc gcacaacccg gcctttgctg cgcgatttcc
     4621 cgacccagcg cgtccatcca aacatcgtgg tcgacggtga ggacttcttc gagcgtctta
     4681 ccgctggcaa actcactcaa atacgaggcc gaagcctggc tgattgtaca accggttccg
     4741 tcgaatgtca catcggctcg gcccgtccga gggtcgacct tcacgtacat cgtcacgaga
     4801 tcgccgcagc ccggattgct gccctgcgcc gacgcgccgg catccggcag cgcccctttg
     4861 cggcgcggat tctcgtagtg atccagtatg aagtcgatct gttcttgccg gtccatcgcg
     4921 ggtcaagcca gccccatctt ggcggcaaac gacttttcca ggcgtcgccg cacgcttttg
     4981 tgtggaatgc ggtcgagcac cggctggaag aacgcgttga cgatcaactg ctcggcgacc
     5041 ggcttcggga caccgcgcgt catcaggtaa aagacttcct cgcgatcgat ctggctgacc
     5101 gccgagccgt gcgtgcagcg cacatcgttc gcctcgatct cgagaccggg gattgaatcg
     5161 gtgcgcgcct cgtggctgag cgccaggttt cggttggcct ggtacgcgtc ggtgcgctgc
     5221 gcgccgggat acacgcggat gttgccctgc cacaccgcgc gcgccttgtc tttgagcgcg
     5281 cccttgtaca aaaggtcgct cctggcgtgg ccggcgacgt gattctgctc ggtatcgtaa
     5341 tccatgtgct gcgtcccgtc gacgaagtac acgcctgaca tcagcgcggt gctgccctcg
     5401 ccgaccagcc gcgagtcgag gaaggatttc gtcaggcggc cgcccaaccc ggccacgacc
     5461 cagtgcagcg tcgcgtcgcg ctcgacggcg gcgcgctcgt gcgtgaagtt gaatacgtcg
     5521 cgcccccagt cttgccagtt cacgtagtcg agctgcgcgc ccggcccggc ctgcatctcc
     5581 accgcgccgc acacgagggc ctgcctgacc tgcgttcgcg atgcatattc gtcgatgaac
     5641 acgacgcgcg ccccacgctc tacgacgatc aaggtgtgag taaacgacgc cgcgtgttcg
     5701 gacagccagg tcgccgcgcg cagcggcatc ggcacatcga cgccggccgg cacgtacagg
     5761 aaagcgcccc cctgccagaa ggccgcatgc agcgccgcga agtagccgtc gttgggcttg
     5821 acgatctcgg tcatgaaata gcgcttcagg agatcgccgt gctcgcgcag cgcggcgtcc
     5881 atgtccgtga agatgacgcc gcgctggcgc agcggctcat ccagccagtt gccgccgacg
     5941 agaccatcga cctgaagcag cgcgccggaa gcatgcttgt ccctcagcac gttgcgcacc
     6001 ggcataggcg tgtgcttgag cgcggttttg agatcgtcgt cctgcgccag cgtcgccacc
     6061 tcctcgaact tcagcgccga gtaatcggtg cgccgccacg cctcgtcgtt tcgggctggc
     6121 agaggcgtat cttgcagaat gcgccaggcg tccagacgca cgtctcgcat ccaatccggc
     6181 tcgaaaggac gagaggcgat cagggcgtcg agcgattcgc gggaaaatcc aagcgcggcg
     6241 ggcgcgaggc cggtctcggc ttcgcgaacc ggctcgctcg ccgcaagcgg caccgccctc
     6301 gcggtcgttt cgtcgtttaa cgtcgtcaca gaggtcaaaa ccattctcct gctattcgat
     6361 gcgcgaggcg caccccatag acgagaggca cgccccgacg atgatgctca ctccgctgcg
     6421 cacaaaccgc acggccaccg ggccctatga ctactcggct atccggctat cccacgctgc
     6481 cttccatctg gagctggatc aggcggttca tctcgaccgc gtactccatc ggcagctcct
     6541 tgaccagagg ctcgatgaag cccgacacga ccatcgagcg ggcggtcacc tcgtccaggc
     6601 cgcggctcat caggtagaac aactgctcgt cgccgatctt gctgaccgtc gcctcgtggc
     6661 cgatggtcac gcggtcctca tcgatctcga tgtagggata cgtgtcggtg cggctctcct
     6721 cgtgaagcag cagcgcgtcg cactgcacgt gagattgcgc gccctccgcg cccttgtgca
     6781 ccttgagcaa gccgcggtaa gaggcgcgtc ccctgttcat gctgatcgac ttggacgtga
     6841 tcttgctgcg agtgtacggc gcgacgtgca cgaccttgcc gccggtgtcc tggtgctggc
     6901 ccgtgccggc gaaggccatc gacagaatct cgcccttggc gcccttgccc atcaagtagc
     6961 acgacggata cttcatcgtc agcttgctgc ccaggttagc gtcggtccac tccatcgtcg
     7021 cgtcctcctc gacgatggcg cgctgggtga ccaggttata gacgttggcc gaccagttct
     7081 ggatcgtggt atagcggcag cgcgcgccct tcttcacgac gatctcgatc acgccggagt
     7141 ggaacgaatc gcgcgagtag accggcgcgg tgcaaccctc aacgtaatgc acttgcgcgc
     7201 cttcgtccac gatgatcagc gtgcgctcga actggcccac actctccgag ttgaggcgaa
     7261 agtaggcctg caaaggcatc tcgacgtgca cgcccttggg cacgtacacg aacgacccgc
     7321 ccgaccatac cgcgctgttg atcgcggcga acttgttgtc ctggatcgga atgatcgtgc
     7381 cgaaatgctc tctgaacagg tcggggtagt gcttgaggcc gtcgtcgctg ctcaggaaaa
     7441 tcacgccctg cttctcgagg ttctcctgaa tgctgtggta gaccatctcg ctttcgtact
     7501 gggcgccaac gccggccaga aacttgcgct cggcttcggg gatgcccagc ttgtcgaacg
     7561 tgttcttgat cgtttcgggc acctcgtccc aactggcctc ggtcttttca gaggggcgga
     7621 tgtagaagta aatctcgtcc aggttcaggc cggacaggtc gccgccccac gtcggcgtcg
     7681 ggcgctggac ggcatgcgca tacgccttca ggcggaattt gcgcatccac tccggctcgc
     7741 ccttcatggc cgagatttct tcgacgacct cacgcgtgaa gcccttgccc gacttgaaga
     7801 cgtgcgtggc cggatcgacg aaaccatact tgtattcctt caaactctgc agattctgtt
     7861 cttgcgtcgc catctcctct ccttgcctgc ctaagcaggc tccgcaacgg atggcagtcc
     7921 cagccgcatg cgaatcgatt cgtagccgcc ctcttccagc tccagggcca gctcgggacc
     7981 tccggtcaac acgatccgcc catcgtacat gatgctgacg acatgcggtt tgatatagtt
     8041 gagcagccgc tgataatgcg tgatgaccag cgcgcccata ttcgggccga gcagcgtgtt
     8101 cacgccctcg gcgaccagct tcagggcgtc gatgtccagc ccggagtccg tctcgtcgag
     8161 aatcgccatt tccggttcca ggacggccat ttgcagaatc tcgttgcgct tcttctcgcc
     8221 gccggaaaag ccctcattga ggtagcggcc tgcaaaagca ggatccattt tcagcatttt
     8281 catcttcgct atcagcagct tgcgaaactc ggggatggag atgcctttct tgcccgccgc
     8341 ctgagtcgcc acgagaggac cgctcgagcc gccgctggcg ccattcccca ccttccggcg
     8401 agcgttgagc gcggtgcgga gaaagttggc gacggtcacg ccagggatgg acaccggata
     8461 ctgaaacgcc aggaacagcc ctagacgcga ccgctcatcg gcgctcaaat taaggacgtt
     8521 ctgacccttc cagacgactt cgccctcaat cacctgataa ccaggatggc ccgcgatgac
     8581 gtaggcgagc gtgctctttc ccgagccatt cggccccatc agggcatgta cctgcccctg
     8641 ctcgacggtc agggtcacgc ccttgagaat ttccttatct tcaatcttca caagcaaatt
     8701 gcggatttcg agcttatcgg ccatgaggcg cttcttcctc caactgcacg gtttgcacgg
     8761 tcgctcagtg ttcgtgcgga ttgtaacacg ccgattcgag tttgtcaata cttataaaat
     8821 aaattgaaat attgacagat tgggtaagtc cgtgtatact ctactcgaat gcgggagttg
     8881 ggagtcggat tcacgacgca tgcaagataa tcgaagcgct gttctcaatc tgctgcggga
     8941 gcgcggcgaa gcgaccgctg ccgatattgc ggtagccctg gacacgacgc ccgtcaacgc
     9001 acactatcac ctgagcaggc tcgagcgcga gggcctgatt gtctcggagc cactccggca
     9061 gggagtaggc cgcccgaagt acgtatactc cctggccggc gcggcccttc agcttgtccc
     9121 gcaatcgacg catcggctcg ccgaccgctt gctcgacgcg ctacagtctc aactgacggc
     9181 gcagcagatc gaggccatat tcaaccacat ggtcgaggac atcactgcgg agcacggcgc
     9241 cgagcttgga gacagatcgc tcgaggagaa gatcgagacg ctcgtcgagg ttctcggcga
     9301 agaaggtttc ttcgctcggg ttgagaagat cgaccaggat tttcaattga cgcagtgcgg
     9361 ctgcccgtac cggtacgtcg ccgcccggca tcccagcatc tgcgcgatcg acttgcaact
     9421 gatcaacacc gcgctcggcg ccgaggtcga gcgccaatcc tggattctga atggcgacaa
     9481 cgtgtgcacc ttccacgtca aagcccccgc gctcgatggg aatccgtagg gagacgacat
     9541 gtccgacatc gccgaaaaca cgacagccga agccggcatc cagtacagcg aacaggaaat
     9601 cggcctgcgc gacgcgctgc gcgacgtggt cgaccccgag ctgggtatga gcatcgtcga
     9661 gctgggattg atccgcaaga tcgactttca tccagatctg actgaaatca cgatgatcat
     9721 gaccaccccg ttctgcccgt acggcccggc gctgctcgaa caaacgcgcc gcaccgccga
     9781 gaagcatctg caaggtccgg tcaagatcac gctgggcatg gagatgtggg agccgtcgat
     9841 gatggagggc ggccctcctg cggaatgggg gctgtactag gccctcgttc agacgcatgt
     9901 tatctcgaac gcacacgcga atgggtgttg gcgcgtatag cacgagcggg ttcgccccgc
     9961 tcgcgctatt tctcgaacag gttccgtccg agggcgcatg accacagaat tcgcgaccgc
    10021 cgtatccgta cccgacgacg agatcgatct ggcgcgggcc gcgctgctct tcgcgcgcga
    10081 cgcttatccc gagatcgatc cggatgcgta cgtgcgacag ctcgacacgt gggccggctc
    10141 tattcgagtc gcagtcgagt cgcgccgatc ggatccatcg ttcggaatcg tgaccgatct
    10201 cctgttcgac aggctgggct ttcaaggcag cgaaccggac tacagtgatc cgcgcaattc
    10261 gtacttgaac caagtcatcg accggcgcag aggactgccc atcgcgctgt ccgtcatcta
    10321 cctggagatc ggctggcgcg tcgggctgcc gctgagcggc gtcgggctgc cgggccactt
    10381 catcatccgc tgcgactcag gatcacgcac ctggttcatc gatccgtttc accagggcgg
    10441 cgtgctcgac gaagaggatt gcgctcgcct gatgcgacga acggcaggcg acctgccctt
    10501 cagccggagc ctgcttcttc ccgtcacacg gcgccagatc ctggcgcgca tgctgcacaa
    10561 cctcagggcg acatatgtgc agcgcgacat gctgcgcgaa gcccaaccgg tgatcgagcg
    10621 actgatcgag ctggacccgg cgtcgggcga acccgtgcgc gatctggggc tgattcattt
    10681 ccgccagggc gcgtatcggc gcgctatcga cctgcttgag tcgtacctgg ggcttgcgcc
    10741 cgacaccgac gacgtcgcgt ccatccggca agtgatcggc gcggcgcgag cggagatggc
    10801 gcgccggaac tgaaccctct ccgtgcgcac gcaaaagatg tcagcatttg cgtgcacatc
    10861 cctctcctgc ttgtcggcga ctttgaagca tgctagaatg ctcatttagc acgggtgcgt
    10921 gagtgtgttc gcgtgagaac gacgaccttc cgccggatgt ccggcgtttt gtttgtcttg
    10981 gcagcgatgg ggctgggtta tgtcgcaggg ctgttgaccc ccgtcgccgg cgagccggcc
    11041 gccaccacgg gcgcgccgtc tggcgcgctg tacgaggagg cctggcagct cgtcgatcga
    11101 catttcttcg gcacgctccc gccgacacag acccgtacct acgcggcgat tcgcgggatg
    11161 ctggctgccc tcggcgatca atacaccgtg ctgatcgagc cgccggctgc ccgtcttgaa
    11221 tcggatcagt tgcgcgggga gttcggcggc gtcggagccg acgtgcgtcg ggacgcggcg
    11281 ggccgcacgt tgctctcacc ctaccctgac ggcccggcgg cccgcgccgg cgtggtcgaa
    11341 ggcgacgaac tgacggcagt cgacggcaag ccggttgcag aactgccacg gctggacgag
    11401 atcgaagcgc tgctgcgcgg cgacgtcgga agccgggtac gcctgacgct cgtccgcgaa
    11461 gggcaggcgt tcgacgtgga agtcgagcgc gcgaatgtcg cgccgccctc gacgatgtgg
    11521 cgagttctcg cagaaacgcc cgacattgga tatatttcta tacgtctgtt caccgatcgt
    11581 acggccgacg aagtgcggca ggccctcgaa gacctgcgcg gccaaggagc gcgcgcgctc
    11641 atcttggaca tccgcgacaa cggcggcggc ctcctggatg cggccgtcga cgtgaccggg
    11701 caattcatcg atggggtcgt cttgatcgag cgcaaacgcg acgagatgga gcgccagttc
    11761 acggccgccg cggacggcgc cgcccgagat atgccgctcg tcgtgctggt caatcacagc
    11821 acggccagcg ccagcgagat cgtcgccggc gcgttgcgtg atcgtcaacg cgccatcctg
    11881 atcggcgagc cgacgtatgg caaaggctcg gtgcagagca tctacgcatt gtcggacggg
    11941 tcttcgctgc acatcaccac agccgagtgg tatacacccg gccatgacga cttcaacggg
    12001 cagggcttgc ggccggacat tccagtggcg cgcagcgaag atgaccgcgc cgccgggcgg
    12061 gacccggcgc ttgacgcggc ggtagcgcat ctgacacaga tacttccata gacggagtgc
    12121 gaaatcccag cttaattggc gcgtgcgggc cgagcagcta agaaaggact tatatggaac
    12181 gcggcacacg taatttactg tatgggttac tcatggtgat cgtcatcgtc gcggtgggat
    12241 cgacgatgta cgtcgtcggt ctgaatgccg gccgcagcca gccggccgcg ccggtcgccg
    12301 ctatcatcag cccgacgccg gacgcgccgt cgtcgactcc cgtgcccggc acgccaacgg
    12361 cagcgaccgg cttggacgat ggcagcatcg acgaggagct gggcatcttt cgagaagcat
    12421 acgatctcat ccagagcgag tttttcggcg agataccgtc tgataacgag attgtctacg
    12481 gcgcgatccg aggcatgctc cagcggctag acgatcagaa tacgactttt ctggaaccgt
    12541 cgatcgccga gctggaccgc gaggcgcgaa cggggagcta cggcggcatc ggcgcgctgg
    12601 tcaacctgag cgaaagccag gcgctcgagc tcgtgcgcgt cttcaaaggc tcgcccgccg
    12661 agcaggaagg catgcgggcc ggcgaccagg tgatcgccgt cgacgaccgc tcgatcgtcg
    12721 ggctgagcct agacgaaatg gtggccctgg tcagaggccc ggccggcagc gacgtcagac
    12781 tgacggtcgt gcgctccggc gtcgaggaac cgttcgaggt cgtggtgacg cgcgcccaga
    12841 tcgagatacc tctcgtcgaa tctcgcatga tcggctcgga catcgcctat atcaatctca
    12901 cccagttcga cgggacggcc acggaacagc ttacccagga aatcgaatcc ctcctggcgc
    12961 gcaacccgaa cggcctgatt ctcgacctgc gcggcaatcc gggcggcttc ctggacgagg
    13021 ccatcaacgt ggccgacctg ttcctggccg aaggggtcgt gatgtacgag cgcgagcgcg
    13081 acggagcgga ggacgtgtac cgcagcgacg acggcgacct ggccgaggag attccgctgg
    13141 tcgttctcgt cgacggcggc tcggccagcg caagcgagat cgtcgccggc gcggtgcagg
    13201 accgcgaccg gggcacgctg attggcactc tgacattcgg caagggatcg gtgcagcgca
    13261 tccacacgct gtcggacagc agcgagctgc gcatcaccat cgcgcgctgg ttcacgccga
    13321 acgaccgcgc gatccacggc gagggtctgg agccagacat cgtcgtcgag ccgggcgaag
    13381 atgcggcaac cgatccgcag ctcgaccggg cgatcgaact cctgcggaca gggcaataga
    13441 gtggggcacg gcacgcccgc tcggagctgg gaatggcaaa gcccaaagcg ccccttgcag
    13501 accgctcggc cgtgaaagtg gtggcgacga accgcgcggc gcgtcacgac tacgcgatcg
    13561 acgagcgatt cgaagccggc attgcgctgc tcggcaccga ggtcaagtcg attcgcgagg
    13621 gcggcgccaa cctgcgggaa ggctacgtgc aagtgcgcgg cggcgaggcg ttcctcgtga
    13681 acacgcacat cgcgccgtac gatccggcag gccgcaccgg ccacgatccg ttgcgctcgc
    13741 gcaagctgct gctgcataag aaggaaatcg cgcggctgga aacagggacc cgcgagcgcg
    13801 gctggacggt cattccgctg aggatgtacc tgaaaaacgg gcgcctcaag atcgaaatcg
    13861 cgctggcgcg tggcaagcgc cagttcgaca agcggcaggc catcgcgaag cgcgatgccg
    13921 agcggcaaat cgatcgctca ctgaaggagc ggcgcagcta agcgcctatc cgaaaaccac
    13981 gcactttaac aaccgaatcg gctggactgt aacactgttt gcgaattctt gatatccttt
    14041 agaaatggag gctttgcatg tcgttagaaa gcgacggtgt cgctcttttc atcgatttcg
    14101 acaatgtgcg ttattccctg ctgaacgagc gcggcaagga aatcgagccg cgcgaactga
    14161 tggcgaaagc gcgaacgtat gggcgggtcg tggccgcgaa agcctacgcc gatttttcgg
    14221 agcacccgct ggagttcaga cgcagcctcg aagtggccgg catcgatgtg cgtgatattc
    14281 ccaagggagg cctgggcaag aataagagca agaaatcctc gccggaaatt gccatggtgc
    14341 tcgacgtctt cgagttgctg ctggacaagc cgaccatctc gacgctgatg ctgatgagcg
    14401 gcgaccgcga tttgatccgg ctggtgacga tggcccgcca ccgattcggc aagcgcgtgg
    14461 tgatctcggg cgtgccggaa accgtcagtc gcgatctggc cgccgccgcc gacgcgctcg
    14521 atccgctcgt gccgccggtg ggcttagagc cagaaatcag gcgggcgccc gagccggccg
    14581 cgctgcctga agaagcgccg gacacgatgg atatcggcga agacgagatc aggctggtcc
    14641 ggtccatcta ccatcaccga gatcgacgct accgctcgtt caatttcctg gccgagaagc
    14701 tggcgtctga ccggaatccg aaccgcgtgg gcgatctgga cgaagccaag acggcgctcg
    14761 aagccgccgt cgccgataga atgctcctgc ccgaagagat cgataccgac atgggctaca
    14821 gcgtcacttc gtattggctc aacgaggaac accccaaggt caagttcgtc ctcagccgca
    14881 tggctgagaa cggcgagcag cctcaaaaca gcgagggcca cgccattgca caataggcgc
    14941 aaccgggcta taatgattgt tgcatgggga tgcactggct tcgacgggga atggtcgagt
    15001 cacgtgtggc aagccggggc gctctgaacc tcgtcaacaa cggagcaaac tataggtgcc
    15061 aaccgcacca actacgcggc tcttcctctc gctgcataag cgggaagaag gcccggtcgc
    15121 ctgcgggtga cccgtccgcc tcgacctcgc gccggtgggt cgagggccgg gcgtgacaat
    15181 gccggcatgc ggcccgcctg acgccgatgg ggcgggccaa agataccatc ggctggctcg
    15241 gacggtacct tgtcggttga ggtcgcccga gtgagacacg agcaaccgac taagcttgta
    15301 ggcatgcgtg gtcaccttct tcggactcgg gttcgactcc cgacatctcc accatgcaga
    15361 cgcccgactt atccagtcgg gcgtctttat tctagcgcaa cgagtcccca ggcacttcca
    15421 gcagctcatc gttcggctgg ggcaacttgc agacatacga ctgtaactgc tcaattggca
    15481 cggccccttg ctgaagcaga gcgtaaacct gcgaccgcgt tgtggcttgc gcgaacggcc
    15541 aggtagcatg ctggcgcacc tcgcgcatgg cggcttcggc caccgattcg cctgactcca
    15601 ccgccccgcc gggcagacac cacactcgga agtcttcccg cctggtcagc aagacttgcc
    15661 ggtcgtgaat gacggcaacc gtcacgccga agtttggcat gcgcccactc aacccgaatg
    15721 cttgaaaccg gattcggcca tggtcagcgg cgcgccctca cgctgaacgc ctgcctccac
    15781 cacttccgca acgtacacga cgtgatcgcc cgacggtact gagcctgtga cgcggcattc
    15841 cagccagccc aagcacgagt cgacgatcgg cgatccggtg agcgggccgg gttgccaggc
    15901 gacgccgtcc gtcttgccgg gcgttttcgc cgacttcttt cccagttggc ctgccaggtc
    15961 gcgctggtc
//
LOCUS       DBNHXB010000154         5188 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_872976, whole genome shotgun sequence.
ACCESSION   DBNHXB010000154 DBNHXB010000000
VERSION     DBNHXB010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_872976"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..614)
                     /locus_tag="AC162G_05995"
     CDS             complement(240..614)
                     /locus_tag="AC162G_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075397.1"
                     /translation="MSVFLRGIITPDYLPLVDKFFLVGPKEDIPVTAILDTGFSGTVV
                     LPHALRAAGQFEFSGLARYELANGEIIPVELYKTTIRIGKRTLAADISFTDSTLGMIG
                     MAFIDGKRAIFDLQRRIFKVLE"
     gene            complement(611..883)
                     /locus_tag="AC162G_06000"
     CDS             complement(611..883)
                     /locus_tag="AC162G_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075398.1"
                     /translation="MQTIKDPDAFARKAEAIYNRKYRDEFESKYRGKIVAIDVQSGDA
                     FVGKTELDAFYKARKKHPRHYFFFLRVGYKATQRITSPHFRKLPAA"
     gene            994..2142
                     /locus_tag="AC162G_06005"
     CDS             994..2142
                     /locus_tag="AC162G_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HIR5075399.1"
                     /translation="MMRSDAELVRRTQLGDRDAFGILVERHRARAVGLAMAMLGDFDE
                     AEDVAQEALYQAYFGLDRLRDPARFGAWLCGIAVNLAKMALRRRRMTVSLEDLDGGRV
                     ARGFRWEAATPESEFEARELRRSVRRAIEQLPEDMRAAVWMHYVEGLSYREIAAMFGI
                     GPGTLRVQAHRARHKLREALLDEWQEYPWREEDTMIEVTVQDVLLWMPKDWEPKPVEG
                     ERHYPEAPPAKRTVMVLKERDGDQALPMWIGPYEGETLTLHLAEYQPMRPLTHDLMAR
                     LLDAAGVTVERVAVSALKSDTYFATVSVRSGQETREVDARPSDAINLAVRTGVPILVD
                     AEVMGQQGIPVVGLPDTLTEMGVQRGRPPEEGYTWRSGREIALAQKCA"
     gene            complement(2184..2495)
                     /locus_tag="AC162G_06010"
     CDS             complement(2184..2495)
                     /locus_tag="AC162G_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024061.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HIR5075400.1"
                     /translation="MESARFTKNNEGFTCVACGLEVPPHPSSSRDHCNHCLTGQHVDL
                     NPGDRLNECRGVLEPIGLQTKSGKTKIVYRCRKCHEQVFNIVAPDDNAELITELARMP
                     W"
     gene            complement(2565..3866)
                     /gene="aspS"
                     /locus_tag="AC162G_06015"
     CDS             complement(2565..3866)
                     /gene="aspS"
                     /locus_tag="AC162G_06015"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007463277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HIR5075401.1"
                     /translation="MERIYGSEIGKHTGERVRLAGWVHNMRALGKINFLTLRDASGIV
                     QVVMTRTELAQLDGALPESVIEVEGTVVAEPQAPGGFEVHNPAITVLNRVSEPLPFPI
                     NKPDLDVHIDTFLNSATVGLRHPKKRAILRLSAGVMGGFRRYLDQHGFVEIASPKIVE
                     SATESGANVFQIDYFGRHAYLAQSPQFYKQMMVGVFERVYEVGPVFRAEPHSTVRHVN
                     EYVSLDIEFGFIRDHFDVMALLTRLIRAMLDHLKAAYAGELDMLGAQMPLAPESFPHI
                     YFPDALQLIYERHGEDSRQESDLSPQHERWLGEWALAERQSDFLFVTGYPMVKRPFYT
                     HPNPADPRYANGFDLLFRGTELVTGGQRLHQYEDYVEALRSRGVTDVKPFEGYLQAFR
                     YGMPPHGGFAIGLERFVMQLIGASNLRETTLFPRDLHRLSP"
     gene            complement(3949..5133)
                     /locus_tag="AC162G_06020"
     CDS             complement(3949..5133)
                     /locus_tag="AC162G_06020"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HIR5075402.1"
                     /translation="MNDKLAFLETELKSLREQGLFNMIRTIGSAQGAWIVVDGRKVLN
                     FCSNNYLGLANHPRMKEAAQKAIERYGVGPAAVRSIAGTLDLHVEMERRMAAFKGVED
                     ALFVQSGFVANQAAIQPLVGKEDVIFTDRLNHASIIDGCRLSSAKIVIYEHCDPADAE
                     AKIKEHLPQHRRGLLVTDGVFSMDGDIAPLDKIHEVCEKHGVISMVDDAHGEGVLGRG
                     GRGIVDHFGLHGKFDVEIGTLSKAFGVVGGVIAGKKVIIDWMRQRGRPFLFSSATTAA
                     DTAACMAAVELLEESTELVDRLWANTRLFKGEMKKLGFDTGRSETPITPVILGENHVA
                     QQFSRKLFDLGVFAMPIVYPTVPKGTARIRVMISAAHEPSDLEQGLEAFQKVGKELGV
                     IA"
BASE COUNT          935 a         1550 c         1675 g         1028 t
ORIGIN      
        1 ccgaattcgt cggatggcag gtcggtccgg atggcgccgt cgggcgcgcg gtacagcgag
       61 cggatcatgt cgcggccttt cgaagagaat gcgccgatcc tatcaaactc cgcgaatcct
      121 ggcaaaccat tctcgaaaga gaaacgggcg cagccgtgtg tcggggcgga ggcacgtttc
      181 gccgagaggg ctatgccttg acgcgaactg ggttccagtg agggtataat tcgcgcggct
      241 tactcgagca ccttgaagat gcggcgctgc aggtcgaaga tggcgcgctt gccgtcgatg
      301 aaggccatgc cgatcatgcc cagcgtggaa tccgtgaagc tgatgtccgc ggcgagcgtg
      361 cgcttgccga tccggatggt cgtcttgtac aactcgacgg ggatgatttc gccgttggcc
      421 agctcgtagc gcgccaggcc tgaaaactca aactggccgg cggcgcgcag cgcgtgcggc
      481 agcacgactg tcccggaaaa gcctgtgtcg aggatcgccg tgacagggat gtcttccttc
      541 gggccgacca ggaagaattt atcgaccagc ggcaggtaat ccggcgtgat gatgccgcgc
      601 aggaagacgc tcatgcagcg ggcagcttgc ggaagtgcgg tgaggtgatg cgctgcgtcg
      661 ctttgtagcc aacgcgcagg aagaagaagt agtgacgggg atgcttcttg cgggccttgt
      721 agaaggcgtc tagctcggtc ttgccgacga aggcgtcgcc gctttgcaca tcgatcgcga
      781 cgattttgcc ccggtacttc gactcaaatt cgtcgcgata cttgcgattg tagatggctt
      841 ccgctttgcg ggcgaaggcg tccgggtctt tgatcgtctg catcgctatc acccagtgct
      901 attttagcac tggctgagcc gcttgacaag gggtcgccgc gatccgtctc acgtaacatt
      961 tcgtctcttg actgcaacaa tatacgacga acgatgatgc gaagcgacgc cgaactcgtg
     1021 cgccggaccc agcttggcga ccgcgatgcc tttggcatcc tggtcgagcg gcatcgcgcg
     1081 cgtgcggtcg ggttggcgat ggcgatgttg ggcgactttg acgaggctga ggacgtcgcg
     1141 caagaagcgc tgtaccaggc ttacttcggt ttggatcggt tgcgtgatcc ggcgcgcttc
     1201 ggcgcgtggc tgtgcggcat cgccgtcaac ctggcgaaga tggccttgcg gcgccggcgc
     1261 atgaccgtct cgctcgagga cctcgacggc gggcgcgtgg cgcgcggctt ccgatgggag
     1321 gctgccacgc ccgaatccga gttcgaagcg cgcgagctgc gccggagcgt gcgtcgcgcg
     1381 atcgagcagt tgccggagga tatgcgcgcg gcggtgtgga tgcactacgt cgaagggctg
     1441 tcgtatcggg agattgcggc catgttcggc attgggcctg ggacgctgcg cgtgcaggcg
     1501 caccgggcac ggcataagct gcgagaggcg ctgctcgacg aatggcagga atacccttgg
     1561 cgagaggagg atacgatgat cgaggtgacc gtgcaggatg tgctgctgtg gatgccgaag
     1621 gattgggagc cgaagccggt ggagggcgag aggcattacc ccgaagcacc gccggcaaag
     1681 cgcactgtca tggtgctgaa ggaacgcgat ggcgatcagg cactgcccat gtggatcgga
     1741 ccgtatgagg gcgagacctt gaccttgcat ctcgccgagt atcagccaat gcgaccgttg
     1801 actcacgact tgatggctcg cctgcttgat gccgcaggcg tgacagttga gcgcgtggct
     1861 gtcagtgcgc tcaaaagcga tacgtatttt gcaacggtgt cggtgcgttc tgggcaagag
     1921 acacgcgagg tagacgcacg tcccagtgac gcgatcaacc tggccgttcg gacgggtgtg
     1981 ccgatactcg tcgatgcaga ggtgatgggc cagcaaggta tcccggttgt cggactgccc
     2041 gacaccctca ccgaaatggg cgttcagcgt ggacgcccac cggaagaagg ttacacctgg
     2101 cggtcggggc gcgagatcgc tttggcgcag aagtgtgcct gaagagaacc ccggtttctc
     2161 cgagaagccg gggttctgcc atcttaccag ggcatcctgg caagctctgt gatcagctcc
     2221 gcattatcgt ccggcgcgac gatgttgaaa acctgctcgt ggcacttgcg gcagcgatag
     2281 acaatcttcg tcttgccgct cttcgtctgc aggccgattg gctccaacac accgcgacat
     2341 tcattcagcc ggtcgcctgg gttgaggtcg acgtgctgtc cggtcaggca gtggttgcag
     2401 tgatcgcggc tgctgctggg gtgcggtgga acctcaagcc cgcaggcgac gcaggtgaag
     2461 ccttcgttat tcttagtgaa ccgggcggat tccatttcac tgcttgacga aggaccaagg
     2521 accatggggt ggccttcgtc cttacgtcgt tggtccttcg tcgtctacgg cgatagccga
     2581 tgaagatcgc gcgggaacag cgtcgtctcc cgcaaattcg aagcgccgat gagctgcatg
     2641 acgaatcgct ccaggccgat cgcgaatccg ccgtgcggcg gcatgccgta gcgaaacgcc
     2701 tgcaggtagc cttcgaatgg cttgacgtcg gtgacgccgc gcgaacggag cgcctccacg
     2761 taatcctcgt attggtgcag acgctgcccg cccgtcacca gctcggtgcc ccgaaacagg
     2821 aggtcgaagc cattggcgta acgcggatcg gccgggttcg ggtgcgtgta gaacggccgc
     2881 ttgaccattg gatagccggt cacgaacaga aaatcggatt ggcgctcggc caaggcccac
     2941 tcgcccagcc agcgctcgtg ctgcggcgac aggtcagact cttggcggga atcctcgccg
     3001 tgccgctcgt agatcaattg tagcgcgtcg gggaagtaga tgtgtggaaa gctttccggc
     3061 gcgagcggca tctgcgcgcc gagcatgtcc agttcgcccg cgtaggcggc tttcaagtgg
     3121 tcaagcatgg cccggatgag acgggtcaat agcgccatca cgtcgaagtg atcgcgaata
     3181 aatccgaatt cgatgtccag gctgacgtat tcgttcacgt gacgcacggt gctgtgcggc
     3241 tcggcgcgaa acaccgggcc gacttcgtac acgcgctcga aaaccccgac catcatctgc
     3301 ttgtagaatt ggggactttg ggccaggtag gcgtggcggc cgaagtagtc gatctggaac
     3361 acgttcgcgc cgctctcggt cgccgattcg acgatcttgg gcgatgcgat ctcgacgaag
     3421 ccgtgctggt caaggtaacg gcgaaacccg cccatgacgc ctgccgaaag ccgcaggatg
     3481 gcccgcttct tcgggtggcg cagcccgacc gtcgccgagt tcaggaaagt gtcgatgtgc
     3541 acgtcgagat cgggcttgtt gatcgggaaa ggcagcggtt cggagacgcg attcaagaca
     3601 gtgatcgccg gattgtggac ttcgaatcct cctggcgctt gcggctcggc gacgaccgtg
     3661 ccctccactt cgatcacgct ctccggtagc gcgccgtcga gctgcgccag ctcggtcctg
     3721 gtcatcacga cctgcacgat gccagacgca tcacgcagcg tgaggaaatt gatcttcccc
     3781 agcgcgcgca tattatgcac ccagccggcc agccgcaccc gctcgccggt gtgcttcccg
     3841 atctccgacc cgtaaatccg ctccacctaa tcctccccgg cgcaatgcat cggccaccag
     3901 atgttcacgc aatcacgtca tcacgtatca cgcatcacgc atcacgtatc acgcaatcac
     3961 acccagttcc ttccccacct tctgaaacgc ctccagcccc tgttcgaggt cgctcggctc
     4021 gtgcgccgca ctgatcatca cgcggatgcg cgccgtgcct ttcgggacgg tcgggtagac
     4081 gatcggcatg gcgaacacac cgagatcgaa cagcttgcgg ctgaactgct gggcgacgtg
     4141 attctcgccg aggatgaccg gtgtgatcgg ggtttcgctt cttcccgtgt cgaagccgag
     4201 cttcttcatc tcgcctttga acagccgggt attcgcccac agccgatcga ccagctcggt
     4261 cgattcttcg agtagttcga cggcggccat gcacgcagcg gtgtcggcgg cagtcgtcgc
     4321 gcttgagaac aggaacggcc ggccgcgctg gcgcatccag tcgatgatca ccttcttgcc
     4381 ggcgatcacg ccgcccacga cgccgaacgc cttgctcagc gtgccgattt cgacgtcgaa
     4441 cttgccgtgc agcccgaaat ggtcgacgat gccgcgcccg ccgcgtccca gcacgccttc
     4501 gccatgcgcg tcgtcgacca ttgagatcac gccgtgcttc tcgcatactt cgtggatttt
     4561 gtcgagcggc gcgatgtcgc cgtccatgct gaacacgccg tcggtcacca gcaggccgcg
     4621 ccggtgttga ggcaggtgtt ctttgatttt ggcctcggcg tcggccggat cgcagtgttc
     4681 gtagatgacg atcttcgcgc tcgacaggcg gcagccatcg atgatgctcg cgtggttgag
     4741 gcggtcggtg aagatcacgt cttccttgcc gacgagtggc tggatcgcgg cctgattggc
     4801 gacgaagccc gactgcacga acagcgcgtc ctcgacgcct ttgaacgcgg ccatgcgccg
     4861 ctccatctcg acgtgcaggt cgagcgtgcc ggcgatcgag cgcacggccg ccgggccgac
     4921 gccgtaccgc tcgattgcct tctgtgccgc ctctttcatg cgcggatggt tcgccaatcc
     4981 caggtagttg ttcgagcaga agttcagcac cttccggccg tcgaccacga tccatgcccc
     5041 ttgggcgctc ccgatcgttc gaatcatgtt gaacagaccc tgctcgcgca gcgacttcaa
     5101 ttccgtttcc agaaaggcaa gtttgtcgtt catctgaagg ctctcattca tctgattgga
     5161 ttgatcagcg acgagtgaca agtggcga
//
LOCUS       DBNHXB010000155         6026 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1376480, whole genome shotgun sequence.
ACCESSION   DBNHXB010000155 DBNHXB010000000
VERSION     DBNHXB010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1376480"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="AC162G_06025"
     CDS             <1..184
                     /locus_tag="AC162G_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075403.1"
                     /translation="IKAIDCGKPVIATAMIAAEPWVDALKRMPGVRTVEVTKQNRGPM
                     AARVLQWLESGEKRDA"
     gene            complement(222..1235)
                     /locus_tag="AC162G_06030"
     CDS             complement(222..1235)
                     /locus_tag="AC162G_06030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR5075404.1"
                     /translation="MKFDTSIGFGVDLNDVSTIARAAEAIGFDALWSNETQHDPFLPL
                     ALIAEHTQRIHFGTAVAIAFARSPIVLAHTAWDLAKQSNGRFILGLGTQVKPHIERRF
                     GMPWGPPVARLREFVHVMRAVWDNWQNGTRLNFRGEHYKVTLMTPFFSPAPIDPVHAN
                     IPIYIAGVGAPLSRLAGEIADGFVVHPLNTPKYLAEVVRPAIEEGARRAGRSASDVVI
                     SGSAFVAFDAAEREAARVQISFYASTPSYRPVLDLHGWGAIGEQLSALASRGRWDEMP
                     GVVSDEMLDAICVAGSWDEVADQVKSRYGGLADRIGFYRPFVPGVDEDHWRVVADRLR
                     VIT"
     gene            complement(1232..2083)
                     /locus_tag="AC162G_06035"
     CDS             complement(1232..2083)
                     /locus_tag="AC162G_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5075405.1"
                     /translation="MPEPRLSRHTVNGRGLQVATSGVGPPLLMLPSILMTHANWSLLR
                     PHLESHMLLIMPDPLGSGRSDKPERAEEYMHGAQADLMLGLLDAMQAREAHVIGSALG
                     GATAMAMAGKARDRIRSVALIEGIFAERQLPLWAAHMRDTLADALMGRMAFGSMKKRV
                     VAEGLAKATMRDAWAGLSKIEQQAAAAAYFDPEADRKGWLGQLRGAGRDVTPLLDGLQ
                     TPILYLRGELSTAGDFLERSVGFLGALPNARVVTIENGAHDLHIQQPKKVAELALAFW
                     REIGERV"
     gene            complement(2140..3114)
                     /locus_tag="AC162G_06040"
     CDS             complement(2140..3114)
                     /locus_tag="AC162G_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5075406.1"
                     /translation="MEYRNLGRTGLKVSRLCMGTMNFGWTAPEENAYAVFDAFRDAGG
                     NFLDTADIYSNWAKDNPGGVAEAIIGKWLKKSSVPRGQIVIATKVRGRMGDGPNDEGL
                     SRKHIMDAAEASLRRLQTDTIDLYQTHWYDENTPIEETLRALDDLIRQGKVRYIGCSN
                     YPAWRLMEALWMSDRLGLARYDSLQTHYNLVHRDEFERELRDVCGMYGIGVIPYAPLA
                     GGFLTGKYRRGEPPPGGTRGQQSGRIKEYMSHSRNFDLLDLLRKMGQARGKTVAQMAL
                     AWMLADPVVTSPIIGANTVEQWNELAGALDVKLSGEEKRELDEWSKWQ"
     gene            complement(3131..4159)
                     /locus_tag="AC162G_06045"
     CDS             complement(3131..4159)
                     /locus_tag="AC162G_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5075407.1"
                     /translation="MKALVTGGTGFLGANLAKGLLERGWHVRILRRSTSPLDAVSDLS
                     VEHAIGDVLDPASLAAAMAGVEVVFHVAAISTYWRSKPDLIYQVNVEGPHRVLDAARP
                     AGVKRVVLTSSGAAVGIVRGRPSNEDDAFHERIARFPYGRSKWLAEELAREAAKKGQD
                     VVIVNPSVVIGPRDINWGSGSIIKEMASRRVPGVPPGGASYVAVEDVVAGHMAAAGKG
                     RSGERYLLTGENLTHRQLADVVARVVGVEPPRWDMPGWLFPPLAFAAGAASRLGVRLP
                     IDATQILLSREFIWFDNAKARRELDLPTPCTPIEQAVRQAYKWYMEKGYLSTSNSKLQ
                     IPNSNSKL"
     gene            complement(4156..4479)
                     /locus_tag="AC162G_06050"
     CDS             complement(4156..4479)
                     /locus_tag="AC162G_06050"
                     /inference="COORDINATES: protein motif:HMM:NF025479.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HIR5075408.1"
                     /translation="MSEPVFYVVTAEVNPAVEAEWCGWWVRTHAPDVLAQPGFMRVTL
                     YRADTQPGEWSRYVAIYEVESHAALEAYFAGDAVNRLRADFQSRYGSVTRVSRQVLSP
                     VQIVE"
     gene            complement(4668..5567)
                     /locus_tag="AC162G_06055"
     CDS             complement(4668..5567)
                     /locus_tag="AC162G_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HIR5075409.1"
                     /translation="MSSTPGGGTEKVRGIIDLTRYKEYVIFVIVTTFLGAQVSGAPLD
                     GRLLLALVANWLAVGFSFMINDVEDADDDALNPAKVQRNPVSAGRLDRRTAYLASFAA
                     AILAGVAFYPLGPVPFWLGVVCLVIGFLYSWRPVRLKRIPIVDLISHSLLLAGLQYLC
                     AYFTFASSAAGAQWIAPFVFVMSVSMYGELFNEVRDLEGDLRAGVTHTAAVVGEKQAH
                     VLMVTLLAVGGISFAYSIVAGLVPWWVLVVLFVLAAIILARPILKMRRGSAVDLVGPL
                     HVPAQIVGALTMIVWVAVGYFAR"
     gene            complement(5590..>6026)
                     /locus_tag="AC162G_06060"
     CDS             complement(5590..>6026)
                     /locus_tag="AC162G_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Kae1-like domain-containing protein"
                     /protein_id="HIR5075410.1"
                     /translation="TRFEFAMPKVDHEFAFSFSGIKTAVLRAVQAYGETDGRGNLPVR
                     VPVPDLAASFQRAVVQQLAEKTVEAADRYGATEILLAGGVSANATLREAMQRRAHVRV
                     RWPPMWLCTDNAAMIGAAAHYRYEAGQRDGWDLDIAPNLKLE"
BASE COUNT         1097 a         2085 c         1837 g         1007 t
ORIGIN      
        1 gatcaaggcg atcgactgcg gcaagccggt gatcgccacg gcaatgatcg ccgccgagcc
       61 atgggtcgac gcgctgaagc gcatgccggg tgtgaggacg gtcgaggtga cgaagcagaa
      121 tcggggaccg atggcggccc gtgtgttgca gtggctggag agcggagaaa aacgtgatgc
      181 gtgaaacgtg agaaacggat cacgcatcac gcatcacatc atcacgtgat cactcgcagt
      241 cgatcggcca cgacccgcca gtggtcctcg tcgacgcccg gcacgaacgg tcgatagaac
      301 ccgatccgat cggccagtcc gccatagcgg gacttgacct gatcggccac ctcatcccag
      361 gacccggcga cgcatatcgc gtcgagcatc tcgtcgctca ccacgccggg catttcgtcc
      421 caccgtccgc gcgaagccaa cgccgacaac tgctcgccga tcgcgcccca gccgtgcagg
      481 tcgagcaccg gccggtacga tggcgtcgag gcgtaaaacg aaatctgcac gcgggccgcc
      541 tcgcgctcgg ccgcgtcgaa cgcgacgaaa gccgagcctg aaatgacgac atcgctcgcc
      601 gatcggccgg cccttcgtgc cccttcttcg atcgcgggcc gcaccacctc ggcgagatac
      661 ttcggcgtgt tcaggggatg cacgacgaat ccgtcggcga tctcgcccgc cagccggctc
      721 aacggcgcgc cgacgccggc aatgtagata gggatgttcg cgtgcacggg gtcgatcggc
      781 gccgggctga agaacggcgt catcagcgtg accttgtaat gctcgccgcg aaagttcagg
      841 cgcgtcccgt tttgccagtt gtcccacacg gcgcgcatca cgtgcacgaa ctcgcgcagc
      901 cgggcgacgg gcggccccca cggcatgccg aagcgccgct cgatgtgcgg cttcacctgc
      961 gtgcctaggc cgaggatgaa ccgcccattg gactgcttag cgaggtccca cgccgtgtgc
     1021 gccagcacga tgggactgcg cgcgaaagcg atcgccaccg ccgtgccgaa gtgaatgcgc
     1081 tgcgtgtgct ccgcaatcag ggccaggggc aggaacggat cgtgctgcgt ctcgttgctc
     1141 cacagcgcgt cgaagccgat cgcttcggcc gcgcgcgcaa tcgtggagac atcattcagg
     1201 tccacgccga atccgattga ggtgtcgaat ttcatactcg ctcccctatc tctcgccaaa
     1261 acgccagcgc cagctccgcc accttcttcg gctgctggat gtgaagatcg tgcgcgccgt
     1321 tttcgatggt gaccacacgc gcattcggca gcgcccccag gaatcccacg ctcctctcaa
     1381 gaaagtcgcc ggccgtcgac agttcgccgc gcagatacag gatcggggtc tgcaagccgt
     1441 ccagcagcgg cgtcacgtca cgcccggcgc ctctgagctg gcccagccat cccttgcgat
     1501 cggcctccgg atcgaaatag gcggcggcgg cggcttgctg ctcgatcttc gacagtccgg
     1561 cccaggcatc gcgcatcgtc gccttcgcca agccttcagc gacgacgcgc ttcttcatgg
     1621 agccgaacgc catgcggccc atgagcgcgt cggccagcgt atcgcgcatg tgcgccgccc
     1681 acagcggcaa ttgccgctcg gcgaaaatgc cctcgatgag ggcaacagac cggatgcgat
     1741 cgcgcgcctt gccggccatg gccatcgccg tcgcgccgcc cagcgccgag ccgatgacgt
     1801 gcgcctcacg agcttgcatg gcgtcgagca ggccgagcat gaggtcagcc tgcgccccgt
     1861 gcatgtactc ttcggcccgc tccggcttgt ccgaccgccc gctgcccaag gggtcaggca
     1921 tgatcagcag catatgggac tcaagatgtg gccgcaggag cgaccagttc gcatgcgtca
     1981 tcaggatcga gggcagcatg agcagcggtg ggcctacccc gctcgtcgcc acttgcaggc
     2041 cgcgcccgtt cacggtgtga cgcgagagtc tcggttcagg catgacggcc gcgcctcgcg
     2101 ccgggaagcg acgtgagctt gcgctccccg taacgaccgc tactgccact tcgaccattc
     2161 gtcaagctca cgcttctcct ccccgctcag cttcacatcc agcgcgccgg ccagctcgtt
     2221 ccattgctcg accgtgttcg cgccgatgat cggcgacgtg accaccggat cggccagcat
     2281 ccatgccagc gccatctggg caaccgtctt gccgcgcgcc tgtcccatct tgcgcagaag
     2341 atcgagcaga tcgaaattgc gggagtggga catatattct ttgatgcggc cgctctgctg
     2401 tccgcgcgtc cctcccggcg gcggctcgcc ccggcgatac ttgcccgtga gaaaaccgcc
     2461 ggccaggggt gcatacggga tcaccccgat gccgtacatt ccacacacat cgcgcagctc
     2521 gcgctcgaac tcgtcgcgat gcaccagatt gtaatgtgtc tgaagcgaat cgtagcgcgc
     2581 caggccgagc ctgtcgctca tccataacgc ctccatcaag cgccaggcgg ggtagttgga
     2641 gcagccgatg tagcgcacct tgccctgccg gatcagatcg tcgagcgcgc gcagggtctc
     2701 ttcgatcggc gtgttctcgt cgtaccagtg cgtctgataa aggtcaatgg tgtcggtctg
     2761 cagacggcgc agactcgcct cggccgcgtc catgatgtgt ttgcggctca ggccctcgtc
     2821 gttcgggccg tcgcccatcc ggccgcgcac cttcgtcgcg atcacgatct ggccgcgcgg
     2881 cacgctcgat ttcttcaacc actttccgat gatcgcctcg gccacgccgc cagggttgtc
     2941 ttttgcccaa ttcgagtaaa tgtccgcggt gtcgaggaag ttgccgcccg cgtcgcggaa
     3001 cgcgtcaaag acggcgtagg cgttttcctc aggcgccgtc catccaaagt tcatcgtgcc
     3061 catgcacagt cgagagacct tcaatccggt gcgtcccaga ttacgatatt ccactcagcc
     3121 ctcttggaag ttagagtttg gagttagagt ttggaatctg gagtttggag tttgaagttg
     3181 acagatagcc tttctccatg taccacttgt aggcttgccg caccgcttgc tcgatcggcg
     3241 tgcacggagt gggcaggtcc agctcgcggc gcgccttggc gttgtcgaac cagatgaatt
     3301 cgcgactgag caaaatctgc gtcgcgtcga tgggcaggcg cacgccaagc cgggatgcag
     3361 cgccggcggc gaacgccaat ggcggaaaca gccagcccgg catatcccag cgcggcggct
     3421 cgacgccgac gacgcgcgcg acgacgtccg ccaactgccg gtgcgtaaga ttttcgccgg
     3481 tcaacaaata ccgctcgccc gatcggccct tccccgccgc ggccatgtgc cctgccacga
     3541 catcctcgac ggcgacatag ctcgcgccgc cgggcggcac gcccggcacg cgccgactcg
     3601 ccatctcttt gatgatgctc cccgatcccc agttgatatc gcgcgggccg atgaccaccg
     3661 acgggttgac gatgacgaca tcctgcccct tcttcgctgc ctcgcgcgct aattcctcgg
     3721 ccagccattt gctgcggccg tatgggaagc gcgcgatcct ctcgtggaac gcgtcgtctt
     3781 cgttcgacgg gcggccccgg acgatgccga ccgctgcccc gctgctcgtg agcacgacgc
     3841 gcttcacgcc tgccggccgg gctgcatcga gcacgcggtg cggcccctcg acattgacct
     3901 gatagatcag gtcaggcttg ctgcgccaat aggtcgagat cgccgcgacg tgaaagacga
     3961 cctcgacgcc ggccatcgcc gccgcgagcg aggcggggtc caggacgtcg ccgatcgcgt
     4021 gctcgacact caggtcgctg accgcgtcga gcggcgaagt cgatcggcgc aggatgcgca
     4081 cgtgccagcc gcgctcgagc agccctttcg ccagattcgc gcccaggaag cccgtgccac
     4141 ccgtgaccag tgccttcatt cgacgatctg aactggcgat agcacttgtc gcgacactcg
     4201 cgtgaccgag ccatagcgcg actgaaaatc ggcgcgcagc cgattcactg cgtcaccggc
     4261 gaagtacgct tccagggccg cgtgcgattc gacttcgtag atcgccacgt agcgcgacca
     4321 ctcgcccggt tgcgtgtcgg cgcgatagag cgtgacgcgc atgaagccgg gttgcgcgag
     4381 cacgtcaggc gcgtgcgtgc ggacccacca gccgcaccac tccgcttcga cagccggatt
     4441 cacttcggcg gtgacgacgt aaaaaacagg ctcgctcact atcgcctcct tcgcgggttg
     4501 ctctcgtcga acatcatccg tctcgcgaac aaaacggcgc gactagagcg gattccactt
     4561 tgatttacgc cggagtcgag gcatcagccg gaaaccgcct aaaggctcga ctccggattg
     4621 gcgtacacgc aatcggaatc cgctctaggg acgccccccg cactcactca ccgtgcgaaa
     4681 taaccgactg ccacccatac gatcatcgtc agcgcgccga cgatctgcgc cggaacgtgc
     4741 agcggcccga ctaagtcgac cgccgacccg cgccgcatct tcagtatcgg gcgagccagg
     4801 atgatcgccg ccagcacgaa cagcacgacg agcacccacc acggaaccag cccggccacg
     4861 atgctgtacg cgaaggaaat gccgccgacc gccagaagcg tgaccatcag cacgtgcgct
     4921 tgcttctcgc cgacgaccgc cgccgtgtgc gtcacgccgg cccggagatc accttccagg
     4981 tcgcgcacct cgttgaacag ttcgccgtac atgctgaccg acatcacgaa gacgaacggc
     5041 gcgatccact gggcgccggc cgcgctcgac gcaaaggtga agtatgcgca caggtactgc
     5101 aagccggcca gcagcaggct gtgcgagatc aggtcgacga tcgggatgcg cttcaggcgc
     5161 accggccgcc acgagtagag gaagccgatg accaggcata cgacgcccaa ccagaacgga
     5221 acggggccaa gcgggtaaaa cgccacgccg gccaggatcg ccgccgcgaa cgaagccagg
     5281 tacgctgtgc gccggtccag ccgccccgcc gacaccgggt tgcgctgcac cttggccgga
     5341 ttcagcgcgt cgtcgtcggc atcctcaacg tcgttgatca tgaacgagaa accgacagcc
     5401 agccaattcg cgacgagcgc caacagcagc cgcccatcga gcggcgcgcc ggacacctgc
     5461 gcgccgagaa acgtggtgac gatgacgaag atgacgtatt ccttgtagcg tgtcaggtcg
     5521 ataatgcctc gaaccttctc ggtccccccg ccgggtgtcg acgacatgac tctcctcctg
     5581 agtctgattt cactccaatt tcagattggg cgcgatgtct aagtcccaac cgtcgcgctg
     5641 gccggcttcg taacgataat gggctgccgc accgatcatc gccgcgttgt ccgtgcacag
     5701 ccacatgggc ggccagcgca cgcgaacatg cgcgcggcgc tgcatcgctt cgcgcagcgt
     5761 cgcgttcgcc gacacgccgc ctgccagcaa gatctcggtc gcgccgtagc gatccgccgc
     5821 ctcgaccgtc ttctcggcca gttgctgcac gaccgcgcgc tgaaacgacg cggccagatc
     5881 gggaaccggc acgcggacag gcaggttccc acgcccatcg gtctcaccgt atgcctgcac
     5941 cgcgcgcagg acggccgtct taatgcccga gaacgaaaag gcgaattcgt gatcgacctt
     6001 gggcatggcg aactcgaacc gcgtcg
//
LOCUS       DBNHXB010000156        12087 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3222108, whole genome shotgun sequence.
ACCESSION   DBNHXB010000156 DBNHXB010000000
VERSION     DBNHXB010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 12087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12087
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3222108"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            426..542
                     /locus_tag="AC162G_06065"
                     /pseudo
     CDS             426..542
                     /locus_tag="AC162G_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013753.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            707..4924
                     /locus_tag="AC162G_06070"
     CDS             707..4924
                     /locus_tag="AC162G_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5075411.1"
                     /translation="MDRLDVYIPMDRRVALARDETLPDRTLGAALFADVSGFTPLTEA
                     VAHELGPQRGAEELTRKLDRIYGTIIDEVHRYGGSVIGFSGDAITCWFNADDGRRATA
                     CALLTQRAIAPQATLRTPGGKVFRISIKVAVTAGPARRFVVGHSAIQLLDVLAGAILD
                     RLAAAEHQAQQGEVVVSAKTLEPFADQVVIRDWRLDSTGERVAVIHDLKRAVPPAPWP
                     VGIAVSGDVARDWVLASVYDRLQSGQGQFLAELRPAAALFLKFSGIDYDRDDDAGKKL
                     DAYIRWVQGVVVRSEGTLLQLTMGDKGSYLYIVYGAPIAHEDDAVRAASAALELRTPP
                     AELRFIQDIRIGLSRGQMRVGDYGAATQRVYAVLGNDANLAARLMMKAQPGQILVTQR
                     IAQAIPADFDIRPLEELLVLKGVSQPLKVFTIGVRGSEQAVRTPRRRSLIAMVGRAEE
                     RAALATQLESLLNRRGGSVIIEGEAGMGKSRLVADLLEQAQASAVRILIGEADAIEKS
                     TPYHAWRPIFTRLFNLDPSLDDSDGDGRGMWQTHVLTRLEEIDADLLRLAPLLNAVLP
                     VDLPENELTVQMAGEVRAANTHQLLVSLLNRVAQSAPLLLVLDDGHWLDSASWALARL
                     ISRDVQPVLFVLATRPLPEPPPAEYTYLIQTAGADVIQLGTLAPIDVDRLICQRLGVR
                     GLPQPVADLIHQKAEGHPFFSEELAYALRDTGLIQIVDGECRLVPGVRDLAALDFPDT
                     IQGVITSRIDHLAPQQQLALKVASVIGRIFAYQTLRDIHPVESDKPLLRDALNILQRL
                     DITPLETSEPDLTYIFKHIITQEVAYNLMTFSQRRSLHRVVAEWCERAYAADLSPFYP
                     YLAYHWRRTLDDQQTDRDLAMRAIDYLERAAEQARLNYANQEVVSFLSDALTLDDRLD
                     RSCKPLRRARWERLLGEAYLGLGQFSKSRQHLEQGLALLGHAIPARRGRLAMSLLGQA
                     LRQAWRRLRPAHVKGSQPVESSLPEDDIVRLEAARALTILVSLYYFANETGLLVNGVM
                     KALNLAEVVGPSPEVAQAYSNLCVIAGVIPLHSLAGIYSRLAQETAQALGHLPTQAFM
                     LLRVNLYRAGVGRWREAEEGLEQALALTERLGDRITGIGGAAVMFLVLARQGKFARMA
                     ALAAETRELAQQVENAAFEVWGLNSHAYSLVLLGQADEALRLAEASLVLVPEAENADR
                     VARLDTYGILTKIHLYQGDLSRVEQAVEILEPLVLRSSPTAHAALNGYDNLAEAALAL
                     WEAEKTTSSGWKSSKYTSQELELKARRACKALHRYARVFPIAKPAALLRQGICDWLAG
                     QFGKAHQNWRKALAAAEWLAMPYQQGLAHDAIGQHLTVDNPARREHLNRALEIFTELG
                     TAYDRARVQAILEQGHAAAYTKDEKGD"
     gene            4972..5949
                     /locus_tag="AC162G_06075"
     CDS             4972..5949
                     /locus_tag="AC162G_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075412.1"
                     /translation="MLALVCLRHAWRHARYLAIAFLVAIVYSYSTEIFAINLIHEYYY
                     NRFLIMICRDITVPWGVNIGCAVRSSCVPLAIPVMEAMIIYAAVQTSKRLELPWTVRP
                     VLNGLLAMTIDLMMDPIVSASVVCQPIILPTPVEPGVGFWVWLLRPEQTVFGVTLPEH
                     LLFGIPLNNFSGWFLGAGIFTFMLEIGWQRIPPGSKGILGDFIVPVAAIPLTLLAFTG
                     VIAAYQWLIKNIFGSEWILVALILAVSILVILRFARRAKRDNPIDPFLLAIPLFFQVY
                     FLAELFATDLYRKQPGLVAFAVVLVPISLLLFSWPYWNRIAPAVKPARA"
     gene            6057..7073
                     /locus_tag="AC162G_06080"
     CDS             6057..7073
                     /locus_tag="AC162G_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HIR5075413.1"
                     /translation="MTKLSRVLDALLQFIRFPAFGFTAMLPLLSADTTGQPLTARQML
                     GIIGVALAFHSFCYVLNDVIDLPLDRTQALRRDAPLVRGLIRPGSALAFALLQVPVAL
                     IVTAWMGGGRESYVALSVGFVLMAVYNLLGKRTALPPVTDVIQGVAWGAMVFFGAGLR
                     PGSMTSLTVYLFAFVVMYIILINGVHGSLRDLTNDLTCGMRSTAILLGARPRNAEQLV
                     IPPRLRLYAYTLQVFTLGLLFWPLFENLFGYSPVAWAATLIALLLLSARCLHILAMIL
                     SPNMRQADLARAVGAYLFASLSSLVVLFALHLEPERLIVLLIACLVPLLPNRVRHVAS
                     ALKA"
     gene            complement(7078..7929)
                     /locus_tag="AC162G_06085"
     CDS             complement(7078..7929)
                     /locus_tag="AC162G_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HIR5075414.1"
                     /translation="MIARLFTYLKTMRLIICLFAAIMALEGYWLAVHRFEVFSLAPIF
                     AAIAAGSGLAFANVLNDILDLEADRINHPHRSLPSGKITPTEASLLAGALCGLSLLSS
                     FLAGEQVFLLAVGLLALSVMYNVWAKKIPILGNFIVALSGALILATGAFIVPSGDFPI
                     FPLLATLAFLLAREFLETVSDAPGDQLMGRSSVATLWGKARVLQICFGLALVSGVLLV
                     IPALWARRPQARLLYLLTVASISILPGFLFIIAIWKDQSLRNIHNVIYRTRIVLFSSL
                     ISLLWLV"
     gene            complement(7945..9147)
                     /locus_tag="AC162G_06090"
     CDS             complement(7945..9147)
                     /locus_tag="AC162G_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR5075415.1"
                     /translation="MPSEQVDVLIIGCGPAGASTALHLVRNDPAWAGRIVMVDQAIHP
                     REKLCGGGVTHIGHNILARLGLRFEPPHLKIREVCLVYRGRSVSLRGAPVLRIVRRDE
                     FDHWLIRIAERLGVCVRQGDAQRVVQVRQADKYVEVATPRGVFHARIVVAADGARSFV
                     RRALKWEGDSHVARLLEVLTSETRQAPEFREGVAEFDFTPMGDGLQGYTWHFPSIASG
                     QPCMNRGVFDSRARPERARADLKQILRESLSQRGRDLGEYELKGHPLRWWDRDVRFAR
                     PRVLLVGDAAGTDPLLGEGISFALGYGEVAAAAIADAFQRRDFSLATYADRLLAHPLF
                     RQLDTRTRLARLLYRIGSPQLIGLIWQVICWRLRFAPWRDPEFTLDEMPAFSLIERID
                     ARNQASKA"
     gene            9876..10733
                     /locus_tag="AC162G_06095"
     CDS             9876..10733
                     /locus_tag="AC162G_06095"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR5075416.1"
                     /translation="MPDRASLIEATLLLDGLAFPESPRWHDNRLWFSDMFTHQVATVA
                     PDGKLDTIVTVSHQPSGLGWLPDGRLLIVSMEDRRLLRLDPEGLTVVADLRDMAPFHC
                     NDMVVDRQGWAYIGNFGFDFFVEPPKATVLILVTPDGQKRIVADEMLFPNGSVITPDG
                     RTLIVGETWAARLTAFTIASDGSLMDRRLWAQLEGAAPDGICLDAEGAIWVASPVSSE
                     VLRVREGGQVTHRISVETQAVACMLGGSDRRTLFILSAPVTRPEIGRAQRRGRIETAQ
                     VEIPGAGLP"
     gene            11012..11992
                     /locus_tag="AC162G_06100"
     CDS             11012..11992
                     /locus_tag="AC162G_06100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HIR5075417.1"
                     /translation="MKALVCTEFGPLENLAVKDVASPRPGPGQVVVDVKASAINFPDA
                     LIVQGLYQVKPPRPFSPGLELSGVVKEIGEGVKDVAVGDRVIAFPGSGGFAEECLAPV
                     EKVMPLPPDVDFELGAAFGLTYGTSLHALKDCGQLRSGETLLVLGAAGGTGSAAVEIG
                     KVMGARVIAAASSDDKLALCKQVGADETINYSREDFRQRLRDLTGNKGIDMVYDPVGG
                     PYSETALRATGWRGRFLVIGFAAGDIPKIPLNLALLKERSVIGVYWGDWTQHDPAGHQ
                     RNVKQLVDWLVTGKIKPVISERISLADAPAAMARLVQRQAKGKIVVLPEV"
BASE COUNT         2351 a         3659 c         3586 g         2491 t
ORIGIN      
        1 tgccagccct tatcctaccc caggctcatt ttccctgaca acctttgcgt gcacacacca
       61 gaaatgaagt cttgacaacg gtcgcttttt gcggtatata gacgccaaca tgtctcgtcg
      121 ggtacattga tacccatgct cgtcgggctg gccgagaata cacggcccgg tggttgaaag
      181 atcggtgatc taggattcag actcataacc cggaggtcct aggttcgaat cctagccacg
      241 ctactccaaa gcatcgcaga tgatgaatct gcggtgcttt ttgtacgtcc aggtggctcc
      301 attgtgtaca ctgcaggatt cgaacctgcg acccgcaggt cgcaggttcg aatacgacct
      361 tgtatgcgtt gtgacaccga agcgcctccg ctaccactcc gtgaacttct gcatttagca
      421 acgggttcgc tgaaggcgtt aatccgaaga tcaagctgat caactggcgc agcttcggct
      481 ctcgcaacct ccagcagttt cgttgccatg tgttggtcgc cttcgaactg gtttcccgtt
      541 aagactcgga gagccgtcat aacatactcg atccatctgg atcgtgaagt taaaccgcgg
      601 ccccttgtct cccctcgttt gtgctatact tttccgcgcc gcacgtgctg cgcaccgctg
      661 aagacttcca tccgcgttat ctcagcttga gaaccagggt gtcacaatgg atcgcctcga
      721 cgtttacatc cctatggatc gccgggtcgc cctcgcccgc gatgagacgc tccccgatcg
      781 cacgttgggc gcggcactgt tcgccgacgt ctcaggcttc accccgctca cggaagccgt
      841 ggcccacgaa ctaggcccgc agcgtggcgc agaggaactc acccggaaac tcgatcgcat
      901 ctacggcacg atcatcgacg aagttcaccg ttatggcgga agtgtcatag gcttcagcgg
      961 cgacgctatc acctgctggt tcaacgctga cgacggccgc cgggcgaccg cctgcgcgct
     1021 gctcacacag cgggcgatcg cgccgcaggc aaccctgcgg acgccgggtg gtaaggtgtt
     1081 ccgtatcagc atcaaagtgg cagtgaccgc tgggccggcg cgccgtttcg tggtcggcca
     1141 ctccgctatc caattgcttg acgtgttggc cggggcgatt ctggatcgct tggccgcggc
     1201 cgagcatcag gcgcaacagg gcgaagtcgt cgtgagcgcc aagacattgg agccattcgc
     1261 tgatcaggtc gtgatccgcg actggcgttt ggacagcacc ggcgaacgcg ttgcagtcat
     1321 ccacgatctc aaacgcgccg ttccgcccgc tccctggccg gtcggtatcg ccgtcagcgg
     1381 cgacgtcgct cgcgactggg tgctggcgtc tgtctacgat cggttgcaga gtgggcaggg
     1441 ccaattcctg gccgagttgc gccccgccgc cgcgttgttc ctcaaattta gtggaatcga
     1501 ttacgaccgg gacgacgatg ccggcaagaa gctggatgcc tacatccggt gggtgcaagg
     1561 ggtcgtcgtc cgctccgagg gcacactgct gcaattaacg atgggcgata aaggcagtta
     1621 cctctacatc gtctacggcg cgccgatcgc tcacgaagat gatgcggtac gcgcggccag
     1681 tgccgcgctg gaactgcgca cgccacctgc cgaactccgt ttcatccaag atatccggat
     1741 cggcctcagc cgaggccaga tgcgtgtcgg cgactacggc gcagcgacac agcgcgtata
     1801 cgccgtgctg ggcaacgacg cgaacctcgc cgcccggctc atgatgaaag cgcagcctgg
     1861 tcagatcctg gtaacccagc gcattgcgca agctataccg gccgatttcg acatccgccc
     1921 gctcgaagaa ctgctcgtct tgaagggcgt gtcgcagccg ctcaaggtct tcacaatcgg
     1981 cgttcgcggg agtgaacagg cggtacgcac gccgagacga cgttcgctga tcgctatggt
     2041 cgggcgcgct gaagaacgag ccgcccttgc cacgcaacta gagagcttgc tcaatcgacg
     2101 gggcggcagc gtcatcatcg aaggcgaggc gggcatgggc aaatctcgcc tggtggccga
     2161 cttgctcgaa caggcgcagg catctgctgt cagaatcctg atcggcgaag cggatgccat
     2221 cgaaaaatcc acaccctatc atgcctggcg accgatcttc acccggctgt tcaacctcga
     2281 cccgtcgctc gacgattcgg acggcgacgg gcgcgggatg tggcagacgc atgtcctcac
     2341 gcgcctggag gaaatcgacg ccgacctcct gcgcctggcc ccattgctca acgcagttct
     2401 accagtggac ttgcctgaga acgaactgac ggttcagatg gccggcgaag tgcgcgcggc
     2461 caacacccat cagctcctgg tgagcctgct caatcgagtt gcgcagtcgg cgccgctgct
     2521 gctggtattg gacgatggtc attggctcga ttccgcctcg tgggctctgg ctcgtctgat
     2581 cagccgagac gtccagcctg tgctgttcgt gcttgccacc cgccccttgc ctgaaccgcc
     2641 acccgccgag tatacctatt tgatccaaac agcaggcgcc gacgtgatcc agctgggaac
     2701 cctggcgccg atcgatgtag acagactcat ttgccagcgc ttgggggtca gaggcctgcc
     2761 gcagccggtt gccgacctaa tccaccaaaa agcagaaggg catccgttct tcagcgaaga
     2821 actggcttac gcgctgcgcg acacgggact gatccagatc gtcgatggcg agtgccgact
     2881 tgtgccaggc gtgcgtgatc tcgccgcgct ggatttcccg gacaccattc aaggcgtgat
     2941 taccagccgc atcgatcacc tggcgcccca acagcagttg gcgctgaaag tcgcgagcgt
     3001 catcgggcgc atcttcgcct atcagactct gcgcgacatc catccagtcg agtccgacaa
     3061 gcctctcctg cgcgacgcgc tcaatatcct gcaacgcctg gacatcactc ccctcgaaac
     3121 ctccgagccg gacctgacct acattttcaa gcacatcatc actcaggaag tcgcctataa
     3181 cctgatgacc ttctcgcagc ggcgaagtct tcaccgtgta gtagcggaat ggtgtgagcg
     3241 tgcttatgct gccgatttgt cgccttttta cccgtatttg gcctatcact ggcggcgcac
     3301 cctggacgat cagcaaacag atcgcgatct cgccatgaga gcgatcgatt atctcgaaag
     3361 agccgccgag caggcgcggc tgaattacgc caatcaggaa gtcgtgagct ttctcagtga
     3421 cgccctgacc ctggacgacc gtctcgaccg gagctgcaag ccgctcaggc gcgcgcgttg
     3481 ggaacgtctg ctgggagaag cctacctggg cctgggccag ttcagcaaga gccgccagca
     3541 tttggagcaa ggtctggcgc ttttgggaca cgcgataccg gccaggcgcg gtcgattggc
     3601 gatgagcctg ctaggacagg cgctccggca agcctggcgt cgtctgcggc ccgctcacgt
     3661 gaaggggtca caaccagtcg aaagctcact gcccgaggac gacattgtcc ggctagaagc
     3721 cgcgcgcgcc ttgaccatat tggttagctt gtactacttt gcaaatgaga ctggcctgct
     3781 tgtcaatggc gtgatgaagg cgctcaacct ggccgaggtt gtcggccctt ccccggaagt
     3841 ggcgcaagcc tattccaacc tgtgtgtcat cgccggcgtg atccccctgc actccctggc
     3901 agggatctac agccgcctgg cccaggaaac tgcgcaagcc ctgggccacc tgcccacaca
     3961 ggcgttcatg ctgctcagag tcaatttgta tcgggcgggc gtcggacgct ggagagaagc
     4021 cgaagaaggt ctcgaacagg cgctggcgct taccgagcgt ctcggcgatc ggattaccgg
     4081 aatcggtggg gcagctgtaa tgtttctggt gctcgcccgc cagggaaaat tcgcgcgaat
     4141 ggcagcgctg gccgccgaga cgcgtgaatt ggctcaacag gtcgagaacg ccgccttcga
     4201 ggtctggggc ctcaactcac acgcctacag cctggtgctc ctgggtcaag cagacgaagc
     4261 cttgcgcttg gccgaagcct ccttggtgct ggtgccggaa gcggagaacg ccgatcgagt
     4321 tgcgcggctg gacacgtacg gcatactgac caagatacac ctgtaccagg gcgacctgtc
     4381 gcgggtcgag caggccgttg aaatcttaga gcccctcgtg ctgagatcgt cgcccactgc
     4441 acatgctgcg ctcaacggtt acgataacct cgccgaagct gccctcgcgc tttgggaagc
     4501 tgaaaagact accagttccg gctggaaaag ctcaaagtac acatcccagg agttggagtt
     4561 gaaagcccga cgcgcatgca aagccctgca ccgctatgct cgcgtgtttc cgattgccaa
     4621 acccgccgct ctgctgagac agggcatttg cgactggctg gcaggtcaat ttggcaaagc
     4681 gcatcaaaac tggcgaaagg cgctggcggc ggctgaatgg ctggcaatgc cttatcaaca
     4741 gggcctcgcg cacgatgcca tcgggcagca tctgacagtg gacaatcccg cgcgcaggga
     4801 acacttgaac cgcgcgcttg aaatcttcac cgaactcggc acagcttacg atcgagctcg
     4861 cgtgcaggcg atcctggagc aaggccatgc tgcagcgtac accaaagacg agaaaggaga
     4921 ttaaaccaga tgccacccct gtcctttctt gtcttcgagg tcagcgtcta cgtgctcgcc
     4981 ttggtctgcc tgcgacacgc ctggcgtcat gcgcgctacc tggccatcgc ctttctagtg
     5041 gctattgtgt acagttactc gacggagatc ttcgcaatca acctgatcca tgaatactac
     5101 tataaccgtt tcctgatcat gatctgtcgc gacataaccg tgccgtgggg tgtgaatatc
     5161 ggttgtgctg ttcgcagttc ttgtgtaccg ctcgccattc ccgtaatgga ggccatgatc
     5221 atctatgcgg cagtacaaac atccaagcga ctggagttgc cctggactgt gcgtccagtg
     5281 ttgaatggcc tgctggcgat gaccatcgat ctgatgatgg atccgatcgt cagcgccagc
     5341 gtcgtgtgcc agccgatcat actgcctacc cccgtggagc ctggcgtggg tttctgggtc
     5401 tggttgctgc gaccggagca aacggtcttt ggcgtcacac tcccagagca tttgctgttc
     5461 ggcataccct tgaacaactt ctccggttgg tttctcggcg ccgggatctt cacattcatg
     5521 ctggagatcg gctggcaaag gattccgccc ggaagcaaag gcatcctggg cgatttcatc
     5581 gtgcccgtcg cggccattcc gttgacgttg ctcgcattta cgggggtgat cgccgcctat
     5641 cagtggctca tcaagaacat attcggatcg gaatggatac tggttgcgct gatcttggct
     5701 gtcagcattc tggtgattct gcgcttcgcg cgacgtgcca agcgcgataa cccaatcgat
     5761 ccctttctcc tggccattcc cctattcttt caggtgtact ttttggccga gctcttcgcc
     5821 accgacctct atcgcaaaca gccgggattg gttgctttcg cagtcgtgct ggttccgatc
     5881 agtctgctct tgttcagctg gccgtattgg aaccggatcg ctcccgccgt caagcccgcc
     5941 agagcttagc ccccggttca tcggtcgcac ggagtgcgag accgaacact ctgaatccgt
     6001 cagggccaca tcacgattgc tcccccagga tgtagtgttc ggcagtgagt gaacccttga
     6061 ccaagctcag ccgtgttctg gatgccctgc tacagttcat tcgctttccg gcgtttggct
     6121 tcaccgcgat gctgcccttg ctcagcgcag ataccactgg gcagccgctt acggcgcgcc
     6181 agatgttggg gatcatcggc gtcgcactgg ccttccacag tttttgctac gtgctgaatg
     6241 acgtgatcga tctaccgctg gatcgcaccc aagcgctacg gagggatgca cccctggtac
     6301 gcggcctcat tcgtccaggg tcggcgctag cttttgcctt gcttcaagtg ccggtggcgc
     6361 tgatcgttac ggcctggatg ggaggcgggc gtgagtccta tgttgcattg agcgtggggt
     6421 tcgttctgat ggccgtgtat aacttgctgg ggaaacgtac agctctgccg cctgtgaccg
     6481 acgtgatcca gggagtggcg tggggggcca tggtcttctt cggggccggc ctgcggccgg
     6541 gttccatgac gagtctcacc gtctatctct tcgcatttgt agtcatgtac atcatcttga
     6601 ttaacggcgt tcacggcagc ttacgcgatc tgaccaacga cctgacttgc ggcatgcgct
     6661 cgactgccat tttgctggga gcgcgaccac gcaatgccga gcagttggtc attccaccgc
     6721 gactgaggct ctacgcgtat accctgcaag tgttcacctt gggcttgctt ttctggccgc
     6781 tcttcgaaaa cctatttggc tacagtccgg ttgcctgggc cgcgacgttg atcgctctgc
     6841 tgctcttatc cgcgcgttgc ttgcacatat tggcgatgat cctctcgccg aacatgaggc
     6901 aagcggactt ggcgcgtgcc gtaggcgctt atctatttgc atccctgagc tccctggtcg
     6961 tgctgtttgc cctgcatctg gagccggagc ggctgatcgt tttgctgatc gcctgtcttg
     7021 tgccgctgct gcccaaccgg gtgcggcacg tcgccagcgc gctcaaagcg tagctggcta
     7081 caccagccac aaaagcgaaa tcaggctaga gaacagaacg atgcgggtcc tgtagatcac
     7141 gttgtgaatg ttccgcaggc tctgatcttt ccagatggcg atgatgaaga ggaatcccgg
     7201 tagaatcgaa atcgatgcga cggtgagcag atagagcagg cgcgcttgtg gacggcgtgc
     7261 ccaaagagcc gggatgacga gcagcacccc ggagacgagg gccagcccga agcagatctg
     7321 caggacgcgc gctttgcccc acaatgtggc gacgctgctg cgtcccatca gttgatcgcc
     7381 cggtgcgtca ctgaccgttt cgagaaactc gcgtgccagc aggaacgcca gcgtcgcgag
     7441 cagaggaaag attggaaagt caccgctggg cacgatgaaa gcgccggtcg cgaggatcaa
     7501 cgcgcctgac agcgccacga tgaagttgcc gaggatgggg attttcttcg cccacacgtt
     7561 gtacatcacc gacagtgcca gcagtccgac tgccagtaga aacacctgct cgccggccag
     7621 gaagctcgac agcaggctaa gtccgcacag tgcgccggcc agcaagcttg cttccgtcgg
     7681 cgtgatcttg cccgacggca aagagcggtg cgggtggttg atccgatccg cttccaaatc
     7741 gaggatgtcg ttgagcacgt tggcgaacgc cagccccgac ccggcggcga tcgccgcaaa
     7801 gataggcgcc aggctgaaga cctcgaagcg atgcacagcg agccagtagc cttccagcgc
     7861 catgatcgct gcaaacaggc aaatgatcaa gcgcatggtc ttcaagtagg tgaacagtct
     7921 cgcgatcatc ctgcacctcc ttggtcaagc tttggaagcc tggttgcgcg cgtcaatacg
     7981 ttcgatcaac gagaaggcag gcatctcgtc gagagtgaac tctgggtcgc gccacggtgc
     8041 gaagcgcaag cgccagcata taacctgcca gatcagcccg atgagctgcg gcgacccgat
     8101 gcgatataac agacgcgcta gccgcgtgcg cgtatccagc tgcctgaaca acgggtgcgc
     8161 gagcagccgg tcggcgtaag tggccaggct gaaatcccta cgttggaagg catccgcaat
     8221 cgccgcggcc gccacttcgc cgtagcccag ggcaaaggag atgccttcgc ccaacagcgg
     8281 atcggtccca gccgcatcac ccaccaatag aacgcgtggc ctggcgaaac gcacgtcgcg
     8341 atcccaccag cgaagtgggt gacctttcaa ctcgtactcg cccaagtcgc gaccgcgttg
     8401 tgatagtgac tcgcgcaaga tctgcttcaa gtcggccctg gctctttccg gacgggcgcg
     8461 gctgtcgaat acgccacgat tcatgcacgg ctgccctgat gcaatactgg gaaaatgcca
     8521 ggtgtaacct tgtaacccgt cgcccatcgg cgtgaagtcg aactcggcca cgccctctcg
     8581 aaactcgggc gcctggcgcg tctccgacgt gagcacttcc aacaggcgcg caacgtgcga
     8641 atcgccttcc cacttcaagg cacgccggac gaagctgcgt gcgccatcgg cggcgacgac
     8701 gatgcgagca tgaaaaacac cgcgtggtgt ggcgacttca acatatttat ctgcctgcct
     8761 gacctgcacg acacgctgcg cgtcaccctg gcggacgcac accccgagcc gttcagcgat
     8821 acgaatgagc cagtgatcaa actcgtcgcg ccgcacgatg cgtaagacgg gcgcaccacg
     8881 taacgagacg ctcctgcccc ggtagacgag acacacctcg cgtatcttga gatggggcgg
     8941 ctcgaagcgc agtcccagcc gcgccagaat gttgtggccg atgtgcgtca cgccgccgcc
     9001 gcacagcttc tcgcgtggat ggatcgcttg atcgaccatc acgatccgtc cagcccaggc
     9061 gggatcgttg cgcaccaggt gcagcgcggt cgatgcgccg gccggaccgc aaccgatgat
     9121 gaggacatcg acttgctcgc tgggcatgca ctaccttaac cctgtcgcag aatcacccaa
     9181 tcaacgccag tcgccgcgcg gcgaccggca tctgtccgaa ctgatgtcgc tcggttgaag
     9241 acgaaagaga ttgtctgaag agaggacagc gatccttggc tcactcgccg aatcttctgg
     9301 cgggcataag tatacggcgc aacggggaaa ttgcaaccga gatagattca agtcaggcac
     9361 tcaatgcctg actccgatgt gctgcccagt tccttttcca atcccgtaaa gctccgccaa
     9421 actccaaggt gcggatttga atggttctgc gacagtacgg cggggccggg catggtctgg
     9481 tgcccgggat tggtttcgtc cacggctgtt gctgcctgcg ccgcatcatc ggccgccaac
     9541 gccaaagcag aactcgacgg aacagtcgtg ctccgtgatc tcgctgtgat ctgacgggtt
     9601 gtgttgaatt tctgagaaat tacgctatac tgagattggc cagtgaagca tgcaacttcg
     9661 ggttcggctg cgctggcagt ggcggcaggc cgagcagagt gcgagcgccc ctccgggccg
     9721 aatgtgtgcg tatctcgctc tgtcactgaa gatttcgctg catttgacgt gctgcacatt
     9781 ctcgtcgaca cgccgttcct cacattatga tgagaatctt tctcggggca ccggatgagc
     9841 ataccgtcgc ccgcccaccc acgtggtgaa ctgccatgcc tgaccgagct tcgctaatcg
     9901 aggcaacctt gttgcttgat ggactggctt ttcccgagtc gccgcgttgg catgacaatc
     9961 gactctggtt ttcagacatg ttcacgcatc aagtggcaac cgttgcgcct gacggcaagt
    10021 tagatacgat tgtgactgtg tcgcatcaac cgtccgggtt ggggtggttg ccagatggac
    10081 gactgttaat agtctctatg gaggatcgtc gtctacttcg tctcgatccc gaaggcttga
    10141 cggtggttgc cgatttgcga gatatggctc ctttccattg caacgacatg gtggttgatc
    10201 gacaggggtg ggcttacatt ggcaattttg ggtttgattt tttcgtggag ccgccgaagg
    10261 ccactgtgct catccttgtt actccagacg gtcaaaaacg tatcgtcgcg gatgagatgc
    10321 tattccccaa cggttccgtc atcactccgg acgggcgcac tttgattgtc ggggagacct
    10381 gggctgcgcg cctcacggcc ttcacgatcg cctcggacgg ttctctgatg gaccgacgtc
    10441 tctgggcgca gctggaaggg gcagctcctg atggcatctg cctggatgca gaaggcgcta
    10501 tatgggtggc ttcgcccgtt agttcggaag tgctgcgagt tcgtgaagga ggtcaggtga
    10561 cccatcgcat ctctgtggaa acgcaggcgg ttgcgtgcat gctgggcggg tcagataggc
    10621 gaacgctctt tatcctttcg gcgccagtga cgcggccgga aatcggacgc gcccaaagac
    10681 gcggtcgcat agaaaccgcc caggtggaga tcccgggggc agggttaccg taatcctgcc
    10741 tggcgagttg ttgcctctgc ccacagtact gccatctgac ttaattagct gcaatcacaa
    10801 gtcacaagag gccggctcaa tatggaacac cagtacgcca ccgttaacgg cttgcgaccc
    10861 tattttgtta gcactggcca cgccttcacg ctttacgttt tacgcttcac gcaacacgca
    10921 ccacgcaaca cggccgtctg gtatgcctgg aaagaccagc tcgacgcatt gccgctcgat
    10981 tgagctcgca acggcctttg aggagaagca gatgaaagct ttggtgtgca ccgaattcgg
    11041 cccattggag aatctggccg taaaggacgt ggcgtcaccc cgaccggggc cgggccaggt
    11101 ggtcgtcgac gtgaaggcga gcgcgattaa ctttcctgat gcgctgatcg tccagggact
    11161 ctaccaggtc aaaccacccc ggcccttttc tccggggttg gaactgtcgg gcgtcgtgaa
    11221 ggaaataggc gaaggggtga aagacgtcgc cgtgggtgat cgcgtcatcg cctttccagg
    11281 ctcgggcggt ttcgccgaag aatgtctcgc cccggtcgag aaggtgatgc ccctgccgcc
    11341 cgacgtggat ttcgagcttg gcgcggcctt cggtctgacc tacggcacgt cgctgcacgc
    11401 cctaaaggac tgcggtcaac tcaggagcgg cgagacactg ctggtgctgg gcgctgccgg
    11461 cggcaccggc agcgcggcag tcgagattgg caaggtcatg ggcgcgcgcg tcatcgctgc
    11521 cgcttcgagt gatgacaagc tcgcgctgtg caaacaggtg ggcgctgacg agacgatcaa
    11581 ctacagtcgc gaggattttc gccaacggct gcgtgatctt accggcaaca agggtatcga
    11641 catggtctac gatccggtcg gcggccctta tagtgagacc gcgctgcgcg ccaccggctg
    11701 gcgcgggcgt tttctggtga tcggttttgc cgccggagac atccccaaga ttccgcttaa
    11761 tctagcgctg ctcaaggaac gctcagtgat cggcgtgtat tggggcgact ggacccagca
    11821 tgatccagcc ggtcaccagc gcaacgtcaa gcaactggtg gactggctcg tcactggaaa
    11881 aatcaagccg gtgatcagcg agcgcatatc gctggcagac gcgcccgccg ccatggcgcg
    11941 gctggtgcag cgtcaagcca aaggcaagat agtggtgctg cctgaagtat gaccgggtag
    12001 cgcctgtgtt gtctcagcca gcgcgggtcg gacgatcgcc atccgtgaat tcacccaatc
    12061 cgcgcttatc accatggagc gattccg
//
LOCUS       DBNHXB010000157         4347 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3222624, whole genome shotgun sequence.
ACCESSION   DBNHXB010000157 DBNHXB010000000
VERSION     DBNHXB010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3222624"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..498)
                     /locus_tag="AC162G_06105"
     CDS             complement(151..498)
                     /locus_tag="AC162G_06105"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5075418.1"
                     /translation="MEVYRQSARAREATAKQAMLQRRETAWAVARRAVDILKKDFGAT
                     RVVAFGSLAHGAWFNSRSDIDLMAEGIAPEAFWRAWCALDRLGPQFEIDLVAGESVSG
                     RLREEIEREGVEL"
     gene            complement(564..1940)
                     /gene="selA"
                     /locus_tag="AC162G_06110"
     CDS             complement(564..1940)
                     /gene="selA"
                     /locus_tag="AC162G_06110"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HIR5075419.1"
                     /translation="MSASDRGDALRRLPSVDRLLARPETAPLVELHGRLLATDAIRAA
                     LDGARAAIRNGGETPPETELIDRASELLRGWTRSRPRAVVNATGVIIHTNLGRAPLSK
                     AALDAVEAAAAGYSDLEYDLPSGERGHRMAAVEDIACRVTGAEAALVVNNNAGAVLLA
                     LSALAAGKGVLISRGQLVEIGGGFRVPDVMAQSGARLIEVGTTNRTHRHDYEMALVTS
                     SVEVAAILRAHSSNFRVIGFTTEVPLNELVEIGHWRGIPVMDDLGSGALLDTAQYGLM
                     HEPTVQESVAAGAALVCFSGDKLLGGPQAGLIAGRRDLVERCKTHPLARALRIDKLDL
                     AALSATLTHYLKGEAEREVPVWRMIGTSVEEIERRAQVLAGALRQTGYAVEVISGQST
                     VGGGSLPGETLPTRLVALTIDSPDRFLAKLRAGDPPVVARIEADRVVFDLRTVLKDEP
                     LLKRLLVG"
     gene            complement(1924..2382)
                     /locus_tag="AC162G_06115"
     CDS             complement(1924..2382)
                     /locus_tag="AC162G_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075420.1"
                     /translation="MSTQSKIRFLRRDASRRFDWIVALVLLVSGAVGLYQVVTTTSPI
                     AGEVAGDLGGPAYAQAFFDSPHVGNVALLLFALLVATLGGAWFVVRLLHWRFRPVHEP
                     AKVWRQSLWVALFVVVGAWLQSSRALTFALGAIIAAGFVLLEVYLNVRER"
     gene            2507..>4347
                     /locus_tag="AC162G_06120"
     CDS             2507..>4347
                     /locus_tag="AC162G_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR5075421.1"
                     /translation="MSIVTAHHVRKSFGAFDVLSDVTLALARGQRAALVGPNGAGKTT
                     LLRILAGLEETSGGAVHRARGLAIGFLPQHAEAELTGDVSLYDKMRAVFAGLEARAAQ
                     LHELELAMAKPGEREAAMDKYSRLHAQFELDGGYTYETRIKQVLGGVGFGEEDYARPV
                     NILSGGQQTRALLARLILQSPELLLLDEPTNHLDVDAVEWLEATLNDYPGAIVIVAHD
                     RYFLDAVVNTVWDLERGIVEEYPGNYSKYLALREERRERQAKEYRRQQEFIAKEEEYI
                     RRNMAGQNTRQAQGRLKRLNRLERLEQPRERKAMRLNLGTDLRSGDIVLRAHDLVVGY
                     LDDRKPLVRCEELLLLRGQRAALWGPNGAGKTTFLRTVLGEVPPLAGEVELGASIDVG
                     YVAQVQDALDPDDSILDVVLAVENMPISKARGLLGRYLFGGDDVFKRIGDLSGGERAR
                     VALAILALKGANVLLLDEPTNHLDLESQEILQDVLSDFAGTMILVSHDRYLVDALATH
                     VWAIEGGALVTHEGNYTDYAAQRAAKARAQGKPARGGVTRARAAKAAQDEERKRQKRV
                     AELETEITVLETRIAELSHQIEKAPPDKVGSLGEEYARVERDLHRRL"
BASE COUNT          828 a         1480 c         1384 g          655 t
ORIGIN      
        1 gcaggttgag tgcaaccgag ttcaggtagg catccggatc gcctgacact gaccgagccg
       61 agtgccagtg ccgttgaata atcgtttggg tccgttcaag gtcttcgatc tccgtccgga
      121 tcctttgtgc gagtgcctgg tatcgagcca tcataactcc actccctcgc gctcgatttc
      181 ttccctcagc cgcccactca ccgactcgcc ggcgacgaga tcgatctcaa attgagggcc
      241 taggcggtcg agcgcacacc acgcccgcca gaaagcctcc ggcgcgatgc cttcagccat
      301 caagtcgatg tccgaacgtg aattgaacca cgcgccgtgc gctaaggagc caaacgcgac
      361 aacacgtgtt gcgccgaaat cctttttgag aatatcaaca gcccggcgcg ccactgccca
      421 ggctgtctcg cgccgctgga gcatagcctg ttttgccgtg gcctcgcgcg cacgagcaga
      481 ctgcctgtaa acttccattt tatcaggagg gattatcgac accggccacc tccacgtgcc
      541 tgcgacatga accgaccatt cagctaacca accagcagcc ttttcaaaag cggctcatcc
      601 ttgagtaccg tccgcagatc gaacaccacc cggtcggctt cgatgcgcgc cacgaccggc
      661 ggatcgcccg cgcgcagctt cgccaggaac ctatccggcg aatcgatcgt cagcgcgacc
      721 agccgcgtcg gcagcgtctc gcccggcagg ctgccgccgc cgactgtcga ctgaccactg
      781 atgacttcca ccgcatatcc cgtctgcctc agcgcgccag ccaacacctg cgcccgcctc
      841 tcgatctcct ctacggacgt gccgatcatg cgccacaccg ggacctcgcg ctcggcctcg
      901 cctttgagat agtgtgtgag cgtcgccgac agcgcagcca gatcgagctt gtcgatgcgc
      961 agcgcgcggg cgagcgggtg cgtcttgcag cgctcgacga gatcgcgcct gccggcgatg
     1021 agtccggcct gaggcccgcc gagcagcttg tcgccgctga agcacaccag cgccgcgccg
     1081 gcggcgaccg actcctgcac cgtcggctcg tgcatcagcc cgtactgcgc cgtgtcaagc
     1141 agcgcgccgg agccaaggtc gtccatgacc ggaattcctc tccaatgacc aatctccact
     1201 aactcattaa ggggcacttc ggtcgtgaac ccgatcaccc gaaaattgct ggaatgtgcg
     1261 cgcaggatcg cggcgacttc gaccgaggag gtcaccagcg ccatctcgta gtcgtgccgg
     1321 tgagtgcgat tcgtcgtgcc gacttcgatc agccgcgccc ccgactgcgc catcacgtcc
     1381 ggcacacgga acccgccgcc gatctcgacc aactggccgc gcgagatcag gacgcccttg
     1441 ccggcggcga gcgcactcag cgcgagcagc accgctccgg cgttgttgtt gaccacgagc
     1501 gcggcctctg cgcccgtcac gcggcaggcg atgtcttcga ccgccgccat gcgatgcccg
     1561 cgctcgccgg atgggagatc gtattccagg tcggaataac cggcggcggc agcctcgacg
     1621 gcgtcgagcg ccgccttaga cagcggcgcg cgccccagat ttgtatgaat aatcacgccg
     1681 gtcgcgttga cgaccgcccg agggcgcgag cgcgtccagc cgcgcagaag ttccgaggct
     1741 cgatcgatca gttcggtttc aggtggagtc tcgccgccgt tccggatcgc cgcgcgcgcg
     1801 ccgtcgagcg ccgcgcggat cgcgtccgtg gcgagcaatc ggccatgcag ttcgacgagc
     1861 ggcgcggtct ccgggcgcgc cagcagccgg tcgacggatg gcaagcggcg cagcgcgtcg
     1921 cctctatcgc tcgcggacat tcaaatacac ctcgagcagc acgaagccgg cggcgatgat
     1981 ggcgccgagc gcgaaagtga gcgcgcggga tgattgcagc cacgcgccga cgacgacgaa
     2041 gagcgcgacc cacagcgact gccgccacac cttcgccggc tcgtgcaccg ggcggaagcg
     2101 ccagtgcagg aggcgcacga cgaaccacgc gccgcccagc gtcgcaacca ataatgcaaa
     2161 aagcagcagg gcgacgttgc ccacgtgcgg cgaatcgaag aacgcctgcg cgtacgctgg
     2221 cccaccgagg tcgcccgcca cctcgcccgc gatcggcgac gtcgtcgtga cgacctgata
     2281 aagtccgacc gccccgctca cgagcaggac aagcgcaacg atccagtcaa atcttcggga
     2341 cgcatcgcga cgaagaaagc gtatcttgga ttgggtactc attgcgtcga acccgcctcc
     2401 aacttccaat ccccaatgac cagtccaagg cctcaacccc accctccact cgattataac
     2461 ccatcacctc gcagccgcga aatcgcgatg gtataatcat cttgagatgt cgatcgtcac
     2521 cgcacatcac gtcaggaaat ccttcggcgc gttcgacgtg ctgagcgatg tcacactcgc
     2581 actggcacgc ggccagcgtg cggcgctcgt cgggccgaac ggcgcaggca agacgacgct
     2641 cctgcgcatc ctggccgggt tggaagagac gagcggtggg gcagtgcatc gcgcgcgcgg
     2701 attggcgatc ggcttcctgc cgcagcacgc cgaagccgag ctgaccggcg acgtctcgct
     2761 gtacgacaaa atgcgcgcgg tgttcgccgg cctcgaagcg cgcgccgcgc agttacacga
     2821 gcttgagctg gcgatggcca aacccggcga gcgcgaggcg gcgatggaca aatacagccg
     2881 gctgcacgcg cagttcgagc tggacggcgg ctacacgtac gagacgcgca tcaagcaggt
     2941 gctcggcggc gtcgggttcg gcgaagagga ttacgccagg ccggtgaaca tcctgagcgg
     3001 cggacagcaa acgcgcgcgc tgctcgcgcg gctgatcctg cagtcgcccg agctgctgct
     3061 gctcgacgag ccgaccaacc acctcgacgt cgacgcggtc gagtggctcg aagcgacact
     3121 caacgactac cctggcgcga tcgtcatcgt cgcgcacgat cgctatttcc tggacgccgt
     3181 cgtcaacacg gtctgggacc tcgagcgcgg gatcgtcgaa gagtacccgg gcaactacag
     3241 caagtacctg gcgctgcgcg aagagcgccg cgagcgacag gccaaagaat accggcgcca
     3301 gcaggaattc atcgccaaag aggaagagta catccggcgc aacatggccg gccagaacac
     3361 gcgccaggcg caaggccgcc tgaagcgcct gaaccgcctg gagcgcctgg agcagccccg
     3421 cgagcgcaag gcgatgcgcc tgaacctcgg gacggacttg cgatcggggg acatcgtgct
     3481 ccgcgcgcac gacctggtcg tgggttatct cgacgatcgc aagccgctcg tgcgctgcga
     3541 agagctgctc ttgctgcgcg gccagcgggc cgcgctgtgg gggccgaacg gcgccggcaa
     3601 gacgaccttc ctcaggaccg tgctgggtga ggtgccgccg ctggcgggcg aggtcgagct
     3661 gggcgcgagt atcgacgtcg gctacgtcgc gcaagtacag gacgcgctcg acccagacga
     3721 ttcgatcctc gacgtagttc tcgccgtgga aaacatgccg atctcgaagg cgcgcggcct
     3781 actggggcgc tatttattcg gcggcgacga cgtgttcaag cgcatcggcg atctgagcgg
     3841 cggcgagcga gcgcgcgtcg cgctggcgat cttggcgctc aagggcgcga acgtcctgct
     3901 gctcgacgag ccgaccaacc atctcgacct tgaatcgcag gagatcctgc aggacgtgtt
     3961 gagcgatttc gcagggacga tgatcctcgt ctcgcacgat cgctatctgg tcgacgcgct
     4021 ggcgacgcac gtgtgggcca tcgagggcgg cgcgctggtc acgcacgagg gcaactacac
     4081 cgactacgcc gcgcagcgag cggcgaaggc acgcgcgcag ggcaagcccg ccagaggcgg
     4141 ggtgacgagg gcgcgcgcag ccaaagcggc tcaggacgaa gagcgcaagc ggcagaaacg
     4201 cgtcgccgag ctggagaccg agattacggt gctcgaaacg cgcatcgccg agctatcgca
     4261 ccagatcgaa aaggccccgc cggacaaggt cggctctctg ggggaagaat acgcgcgcgt
     4321 cgagcgagac ctgcaccggc ggctgga
//
LOCUS       DBNHXB010000158        15251 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_538615, whole genome shotgun sequence.
ACCESSION   DBNHXB010000158 DBNHXB010000000
VERSION     DBNHXB010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 15251)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15251)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15251
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_538615"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="AC162G_06125"
     CDS             <1..479
                     /locus_tag="AC162G_06125"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR5075422.1"
                     /translation="PANTGFNIPVTVVPELAAHDNIVGIKDSAGDINQLAEMVRLTRD
                     MNFEVLTGNYGALLPAMTFGVRGAILAVANVAPAEVVTLFEAARAGRWAEAGEMHLRL
                     LPIARAVTSQHGVPGVKAALDALGYFGGMPREPLLPVSTATREAIEGILREARLLP"
     gene            906..2183
                     /gene="serS"
                     /locus_tag="AC162G_06130"
     CDS             906..2183
                     /gene="serS"
                     /locus_tag="AC162G_06130"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR5075423.1"
                     /translation="MLDLTYIREHPDQVKAAMVVVGAPDAPIDRILALDVRRRVLLTE
                     VETLKAERNRVSKEIGKMKDAAEREAQKERMRQVGDQIAALDRQVGEVERELNDLMLT
                     VPNLPDPSVPPGKDESENRVSKVEGEPKQAKDFGFDPLPHWDLGVRLGIIDFERGVKI
                     SGSRFYLLAGAGARLQRALIAWMIDVHVGQGYTEVYPPYLVKTECLIGTGQLPKFADN
                     QFHDETDDLWMIPTAEVPVTNMYRDEILDGERLPIDHVAYTACFRREPMSAGRDTRGI
                     KRGHQFDKVEMVKLCTPETSSDEFDRLLEDAEEICRLLKIPHRVVQMVTGDLSFAAAR
                     KCDVEMWAPGCGEWLEVSSVSDFKDFQARRANIKFRRAKGAKPEFVHTLNGSGLALPR
                     TLIAVMENYQQKDGSIVVPDVLRPYMGGLEVIR"
     gene            complement(2278..3501)
                     /locus_tag="AC162G_06135"
     CDS             complement(2278..3501)
                     /locus_tag="AC162G_06135"
                     /inference="COORDINATES: protein motif:HMM:NF022702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HIR5075424.1"
                     /translation="MCATRQAACLAAAGLCGLMLALSGCQSLPDTPAASQTPGFASTT
                     TPLPQILITPTPQKPTLPPASPTPTADTRLISTFDPDVNPLTGEKVSDPAVLQRRPLA
                     IKVANSNEPGVRPQSGVSFADLVFEHVAEGGITRWTAIFLGKTPPAVGSNRSCRIIDA
                     DLPAMYKSLLACSGFSGGTREYYIKPTEFHAARRVLSPDFGDSAPMFYRTGSAAPPHN
                     LYVDPVAVWEEATRRGINEPQELAGMIFSTAPLGPGSPASHVVIPYGAERAEWRYDPA
                     STACSSLGGCYLRWSDGAAHTDALNGQQLSAANVVIVYANHVEDKRYFEDVALGGHYS
                     VQIQIWNDPSNPSLPGGRAQILRDGQVFEGRWSRPGRFDMLTFLDAEGNPIPLKPGTT
                     WFQMVRLDTTVQVEP"
     gene            3701..4537
                     /locus_tag="AC162G_06140"
     CDS             3701..4537
                     /locus_tag="AC162G_06140"
                     /inference="COORDINATES: protein motif:HMM:NF025690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HIR5075425.1"
                     /translation="MALKGNLRDFSTTQLLNLINLARKTGLLVVESKNGAAQVFFREG
                     KLVYAAYGGQDGRLTSMLVRSGKLTPAQANKINEQPEARNNRELGALLIQSGHLTQSD
                     IVASVKAHVLDVVYRLFSWTEGTFRFEPSQLPPEDRTIQVRVDLENVIMEGSRRIKEI
                     ERLREELPNLDVALRFTDRPDARLRNINLSVDEWRVVSFINPKNSIRQIAQASNMSEF
                     QIRKIVYGMLQAGLVEVMTPLGRAPREAAPAAQAGAPPAQVTMTVKRPVIERLIRFFE
                     RK"
     gene            4674..5213
                     /locus_tag="AC162G_06145"
     CDS             4674..5213
                     /locus_tag="AC162G_06145"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR5075426.1"
                     /translation="MQAVKMVVTGPFAAGKTEFIRTISEIDVVSTERKISSPAERIKD
                     QTTVAMDFGRITIDEDLVLYLFGTPGQKRFDFMWEILSEGMLGFIVVVDSSRPETFRE
                     ARSILDTFRGYALTPYVVAANKQDHEDAWSPEDLRIALKINSDVKVLPCVARDRESVK
                     SALLELLYSILDLMDSEGV"
     gene            5285..6070
                     /locus_tag="AC162G_06150"
     CDS             5285..6070
                     /locus_tag="AC162G_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5075427.1"
                     /translation="MSSIVTSQGVVHYEAYGRGRPVILLHGWINSWGVWQEEMIQLAD
                     RGGYKVYALDFWGFGESAKSKTPPFKIASYVDMVDQFMETLGIFRAPVLGHSMGGTVA
                     LRLALDRPERVTRVACVGSPIHGNSLNFYLKLAGIDWIAHTMWRAPVLLRLMILINTR
                     GDSERVRKMILRDVEKASIESFFRSIGDLHSTDLRQPLRRLAIPALGVYGKSDNIVNP
                     DQARVLAETVHDARVHVLGRSRHFVMLDEPEAFHGIISEFLRN"
     gene            6072..7340
                     /locus_tag="AC162G_06155"
     CDS             6072..7340
                     /locus_tag="AC162G_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012360.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HIR5075428.1"
                     /translation="MRKYIIEDTRLISPFNEKARDLRILNKPLWLAQRDALASYTREE
                     RPTVRSLEEVPNHRDEDRVETIVYRDNLFFDAPFMDEFIRRAREQHKACRVAFALNDK
                     AITTHALKLQSGIHREGDAYLADLWYFPYGRESNVRPLLIDTQAREIGYYNIPKYIAP
                     NQGDLTFQVPQRAFLSIEHWSHVYVANSPFGVFAEGARMEKQIERLSFKLRVLWRGLV
                     ERRQVLTTRALVKKGRNVHIDPSAVILGPVTLGDNVVIQPGAVIDNCHIGNNVTVSHG
                     CVLMLSVVGDGCFLPWRASLFLTTLMENSMVAQNTCLQMCVVGRDTFIGAGNTFTDFN
                     LISKPIRVMHRGRLEEAGMPVIGGCVGHNCRIGSGFVIYPGREIESDVMLTADRAERV
                     IVKNITYRQSDHHEIPGADRLYPAQYRPTA"
     gene            7340..8473
                     /locus_tag="AC162G_06160"
     CDS             7340..8473
                     /locus_tag="AC162G_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR5075429.1"
                     /translation="MDNFAFIIHPIDPKRDVQRKFPALGKVLPVPAIDFFSRFFPPVY
                     ISHVTGARSAAHGREIEGWLVACPFTPQRMMTLPAETVYAKIVKTARLAQSRGARLVG
                     LGAYTSVVGDAGITISRRVDVPVTTGDSYTVAMADAAVREAARRMEIDLGRATVAVVG
                     ATGAIGAVGAELLARRAPQLILIGKRADRLLRVKARCEAAGADVQVAADVAAIRQADV
                     ILTVSSAGDAIIAPHHLKPGAVVCDVARPRDVSRRVVEERGDVLVVDGGIVEVPGEVD
                     FHFDFGLPPRMAFACIAETMALAMEGRYESYTLGKDLTLSQVEAIDDIGRRHGFRLAG
                     FRSFERAVTDEQIEHIKRNAHVARPPSGSPRSDGGRERRVARA"
     gene            8485..9414
                     /locus_tag="AC162G_06165"
     CDS             8485..9414
                     /locus_tag="AC162G_06165"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HIR5075430.1"
                     /translation="MGKGRILVVEDDTDISNMLRIYFTSQGYEVVVAGRGGEALERCR
                     QQLPNVIVLDIMLPDIDGYEVCTQLRTNLRTSHVPIIFLTQKDERSDRIAGLELGADD
                     YITKPFDIEELRLRVQGAMRRAERENLTNPTTGLPSGRLNEEQLRLLMRKQDWGLLYI
                     GIEGLEDFNNAYGFLAGDDVLRFTALLLGETLDEGGAPDDYIGHVGGDKFMVITKMDR
                     ARTLADALKARFANGILSHYSFIDRDRGYVVIRDPDGKDRQAPLMRMCVGLIRSDAQE
                     FTDIREITEAAAEARRVDCASLPSLGAGSPPET"
     gene            9481..>15251
                     /locus_tag="AC162G_06170"
     CDS             9481..>15251
                     /locus_tag="AC162G_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5075431.1"
                     /translation="MTKRIGFRGSIQVKLWRLFVTGMAVAGMLIAAIVLINSGAMTSD
                     PLRAILGIGVLIAAVATISLAGVFVRARIVAPIESLREGAAAFAAGNLAYRIHTDTGD
                     ELQSLAQEINDMSAALQQSQARLAAMAREREREAEFAHARMREMTTLIRSGRAITSLD
                     LKDVLSRLAYEAARAVQGDRCSIYVLDSRQRRLVLRGEWEATPLFPPPVLTGGAGGRP
                     AGNGTDRFSNSNSNSNSNLQAVVGDVAYEWGEGVVGWVALAEKPIFLANAQADKRFVA
                     KAPNDGEIAALIGVPVHSDSAVVGVLQASTRPGTPAFDVSDQRLLSTFAAQAAAAIKN
                     SYLYEIERRRAKEMSIIAEIIRAISSSLDLDATLNSILSSVRELVSYDLAEITLWDPQ
                     AEVLRTRGRGADPKYDEYSRTAGGVYHLGQGITGWMAGHRKPLLLPDLAVSEVRPAVD
                     LEQFPIRSVVGAPMISAQQLVGTIELASYTAHAFNESHLDTLRTIAAQAAVAIQNARL
                     FIESRQRAEEAAGLFRVASIAASALEPNEILKQIMAEAAQLMGTQLGVVMLYRPETQR
                     LEAHPTALFGAPVERVLDMKIDTRRPAFLHSVFRSGRVFRSDDAPRDRRILREYRPFV
                     ERFGARRTLSAPLVVRDTPIGEVHMARAAGAPFDLDDEQRLMTLATLLAGVIETNRLA
                     IERQERLDELQGLVEISQALSALTDASTDKTLTLTPTPTLTPTLTPLQQVYSQITRSI
                     AERIRVQVAGLLLFDPRREMLVSQPPFYGVPDDALEHYVIPVTQGSGLHRLWTEEDTW
                     ISNDVPNDPLTREAGLDRLARTLGVERALFAAMKIGTRRIGVIQVSNKHDGQPFGEED
                     ARLMSIYATHIAAVVENARLYAQTDVRLQQRVEELTALSNISQELNATLDQERILDLV
                     LNEAGRSTGATRGAVALVPPSAHPPSERGEPEGGQAELVLAAMLGYTPEEIERARLLA
                     QRVGQGIIGAVIEMGASIVVDDVRRNPDYLQVSEQTRSELCVPIRYALEVVGAINLES
                     PSVGYFTRDHVAFLKALAAHAAIAIGNTQRYEEQLRRGDLLRRRAEQLANLFEIGQAF
                     RSDRPLLEVLEDVVHAVQQTAGFNIVLLSVLEGDPPALERVAAAGLPVARLEEMKKVR
                     TPWESVGAIMHDAFRISQSYYVPAEHTAVTSQLDTWPPFKADIVPRASGRWHEQDLLF
                     TPLRASGDRILGIISVDEPFEGRVPDRATIETLELFANQAAIAIENARLLEDLQQRID
                     SLTLFNQVSRTISARLDLDGLLATIVDASIELAKANTATIFIRDDAGVFRPRKSHGWR
                     PEQIAHLNWAEGEGLVGKVARDGHALIVPDAKADPLFTPDPTDGAIGAMLLVPIAVGG
                     TVIGVLSVDKPLPRSFSNTDLLMLSTLSDQAAIAIANSRLFDEVRRFSFELEERVRQR
                     TEELARANADLTLERDRVETLYRITSELSMSLDLDRVLNRALALVNEAVGTRRSSILL
                     VDQNSDRLIHRAAMGRGEYLPPGGRPTRFRRDEGLAGWVIRNRMPAIVADIRVDDRWL
                     EPSPPAPLPLQTTPEPSPPAPLPSRATGEGSASSREYRSALAVPLIAGDDALGALLLL
                     HPEAGYFEESHLRLVEAAATQAATAINNAALYGYIRESAERLGAMLRDKQIEAAKAQA
                     ILESVADGVLVSDASETVILFNAAAERILGRARETVLNRPTADIIGLYGASGAQWEEQ
                     IQRWRSGPESRRSVPSLATNVHFEDENRHVRVSIAPVTGPGDELLGTVSVVRDITAEV
                     EADRTTRDFVSNVSHELRTPMTSIKGYADLLLMGAAGSLNENQERFLNIIRSNAKRLE
                     TLVADLLDINRIDSGRAMLDIKQVSMVAVLEQVLTSLHERIEQKRLSIKTELPEGDSL
                     IVLGDQARLLQVLTNLVSNSHQYT"
BASE COUNT         2704 a         4927 c         5004 g         2616 t
ORIGIN      
        1 tgccggcgaa cacggggttc aacatcccgg tgacggttgt gccggaactg gccgcccacg
       61 acaacatcgt gggcatcaaa gacagcgcgg gcgacatcaa ccagttggcg gagatggttc
      121 ggctcacgcg ggacatgaac ttcgaagtgc tgacgggcaa ctacggcgcg ctgctcccgg
      181 cgatgacctt tggcgtgcga ggggcgatcc tggcggtcgc caatgtcgcg cccgccgagg
      241 tggtgaccct gttcgaggcg gcgcgcgccg gccgctgggc ggaggccggc gagatgcatc
      301 tgcgcctgct gcccatcgcg cgcgccgtga cgagccagca cggcgtgccg ggagtcaagg
      361 cggcgctgga cgcgctagga tatttcggcg gcatgccgcg cgagccgctg ctccccgtat
      421 cgacggccac tcgggaagcc atcgagggca ttctgcgcga agctcgcctg ctgccctagc
      481 cgaataacgc atcacggtaa cttctcagcc gtcattgcga gaagtccatc actctggcgt
      541 gatgcgtaac tggagaaact gttccggcca gtgttattcc gagaatccca catcgccggg
      601 tggcgatatg cctgaaatca gaaacgtacg cacggcacgt gtcatgctga gcatctgttc
      661 gcgaagcatc tctgccacgg ctggcaacgt tcgctgtcag gggtagagat tcttcacttc
      721 gttcagaatg acacagggcc aggtttctgg gtagcgccgt ttgcgacgaa gcaatctcct
      781 gcacgaatag cgatgctcgc tgatgggcac ggagattgct tcgcaaaaaa cgctcgcaat
      841 gacgcgggct gagtagtcac gcgtcacgca acacgcaaca cgccaacgta gttgaggagg
      901 atctcatgct agacctaacc tacattcgcg agcaccccga ccaggtcaaa gcggcgatgg
      961 tcgttgtggg cgcaccggac gccccgatcg atcgcattct ggcgctcgac gtgcggcggc
     1021 gcgtgctcct gaccgaagtg gagacgttga aggccgagcg gaatcgcgtg tcgaaggaga
     1081 tcggcaagat gaaagacgcc gccgagcgtg aggcacagaa agagcgcatg cgccaggtgg
     1141 gcgaccagat cgccgcgctc gacaggcagg tcggcgaggt cgagcgggag ctgaacgacc
     1201 tgatgctgac cgtgccgaac ctgccggacc cgagcgtccc gccgggaaag gacgagagcg
     1261 aaaaccgcgt gtcgaaagtg gagggcgagc cgaagcaggc gaaggacttc ggcttcgacc
     1321 cgctgccgca ttgggatctc ggcgtgcggc tgggcatcat cgacttcgag cgcggcgtga
     1381 agatcagcgg ctcgcgcttc tatctgttgg cgggcgcggg cgcgcgcctg cagcgggcgc
     1441 tgattgcgtg gatgatcgac gtgcacgtcg ggcagggcta cacggaagtg tatccgccgt
     1501 atctggtcaa gacggagtgc ctgatcggca ccgggcagct tcccaagttc gccgacaacc
     1561 agttccacga cgagacggac gacctgtgga tgatcccgac cgccgaggtg cccgtcacga
     1621 atatgtatcg cgacgaaatt ctcgacggcg agcggctgcc gatcgaccac gtcgcctaca
     1681 cggcctgctt ccgccgcgag ccgatgtcgg ccgggcgaga cacgcgcggc atcaagcgcg
     1741 gccatcagtt cgacaaagtc gagatggtca agttatgcac gcccgagacc agcagcgacg
     1801 agttcgacag gctgcttgag gacgccgagg agatatgccg gctcctcaag attccgcatc
     1861 gcgtggtgca aatggtcacc ggcgatctgt catttgcggc ggccaggaaa tgcgacgtcg
     1921 agatgtgggc gccgggctgc ggcgagtggc tcgaggtctc gtcggtcagc gacttcaagg
     1981 acttccaggc gcgccgcgcg aacatcaagt tccgccgggc gaagggcgcc aaaccggagt
     2041 tcgtccacac gctgaacggg tcgggcctgg cgctgccgcg cacgctgatc gccgtgatgg
     2101 agaactatca gcagaaggat ggcagcatcg tcgtgcccga cgtgctgcgc ccctacatgg
     2161 gtgggttgga ggtcatccgc tagagcggat tccactttga tttacgaacc tgtaacgggt
     2221 ttgcaaccca tgcgcacacc tgcccgtgct acccttgaaa tggggccgca cggagaccta
     2281 cggctccacc tggacggtcg tgtccagccg caccatctgg aaccaggtcg taccgggttt
     2341 cagtggaatg gggttgcctt ccgcgtcgag gaaagtgagc atgtcgaagc ggccgggccg
     2401 gctccagcga ccttcaaaaa cctggccgtc tcgcagtatc tgcgcccgac cgccgggcag
     2461 gctcgggttg ctggggtcgt tccagatctg gatctggacc gagtagtgac cgcccagggc
     2521 gacgtcctcg aagtagcgct tgtcttcgac gtggttggcg tagacgatga cgacgttcgc
     2581 tgcgctgagc tgctgtccgt tcaaggcgtc ggtatgcgcg gcgccgtcgc tccagcgcag
     2641 gtaacaaccg cccaggctgc tgcaagcggt cgacgccgga tcgtagcgcc actcggcgcg
     2701 ctcggctccg taggggatga cgacgtgaga cgccggcgag cctggaccga ggggtgcggt
     2761 cgagaaaatc attccggcca gctcctgtgg ctcgttgatg ccccggcgtg tggcttcttc
     2821 ccaaacggcc accgggtcga cgtacaggtt gtgcggcggg gccgccgagc cggttcgata
     2881 aaacatcggc gcgctgtcgc cgaagtcggg ggagagcacg cgccgcgccg catgaaactc
     2941 ggtcggcttg atgtagtatt cgcgtgtgcc gccgctgaag ccggagcagg ccaggagcga
     3001 cttgtacatg gctggcaagt cggcgtcgat gatgcggcag gatcgattcg agccgacggc
     3061 cggcggcgtc ttgcccagga agatcgccgt ccagcgcgtg atacctcctt cggcgacgtg
     3121 ctcgaacacc aggtcggcga aactgacgcc ggattggggt ctcacgcccg gttcgttcga
     3181 gttggcgact ttgatggcca gcggccggcg ctgcagcacg gccggatcgc tgaccttctc
     3241 gccggtcaag gggttcacgt cggggtcgaa cgtcgatatg agacgtgtgt ctgccgtcgg
     3301 cgtagggctt gcgggcggca gagtcggctt ttggggcgtc ggcgtgatca ggatctgcgg
     3361 cagcggtgtg gtcgtggacg cgaaccccgg cgtctgcgac gcggctggtg tgtcgggcag
     3421 gctctggcaa cctgacagtg caagcatcag gccgcacagg ccggcagcgg ccaggcaggc
     3481 ggcctgacgg gtggcgcaca tgcttcctcc ggagcggcag tggttagaag caaattatag
     3541 cataccgccc tatttggcct tgagaatggc gggagggtca aattgacttg ccattttaga
     3601 gcaggtataa tgcgcccgat gtgtcggcga tgggttcagg cgtcgtcgcg ggagggtcgg
     3661 cgatgccttg tagttgattg catgagtgaa ggcggtttgc atggcattga aaggcaatct
     3721 tcgtgacttc agcacgaccc agttgctcaa cctgatcaac ctggcgcgca agacgggcct
     3781 gctggtggtc gagagcaaga acggggcggc ccaggtcttc ttcagagagg gcaaactggt
     3841 ttacgccgca tacggcggcc aggacggccg gctcacctcg atgctcgtcc gttccggcaa
     3901 gctcacgccc gcgcaggcca acaagatcaa cgagcagcct gaggcgcgca acaatcgcga
     3961 gctgggcgcc ctcctgattc agtccggcca cctgacgcag agcgacatcg tcgccagtgt
     4021 gaaagcccac gtcctcgacg tggtctaccg gctcttttcc tggacggagg gcacattccg
     4081 cttcgagccg agccagttgc cgcccgagga tcgcacgatt caggtcagag tggacctgga
     4141 gaacgtgatc atggaaggaa gccgccgcat caaggagatc gagcggcttc gcgaagaact
     4201 gcccaacctg gacgtggctt tgcgtttcac ggatcgcccc gacgcgcgct tgcgcaacat
     4261 caatttgtcc gtggacgaat ggcgcgtcgt gtcgttcatc aatcccaaga actcgattcg
     4321 ccagatcgct caggcgagca acatgagcga gtttcagatt cgcaagattg tgtacggcat
     4381 gctgcaggcc ggcctggtcg aagtgatgac gccgctcgga cgcgcgccgc gcgaggctgc
     4441 gccggcggcg caggccggcg cgccgccggc gcaggtgacc atgaccgtca aacggcccgt
     4501 catcgagcgc ttgatcaggt tcttcgagag gaaataggct cggccgccga tgcgcagtag
     4561 gcagacgccg agcgacggag acaactcggt tcttctccac ccctctccca ccgcagacgg
     4621 cgggagaggg gccgggggtg agggccgcat gtgagagcga gggtagggga cttatgcagg
     4681 cagtcaagat ggtcgtcacc gggccgttcg cagcaggcaa gacggaattc attcgcacga
     4741 tcagcgagat cgacgtggtc tccaccgagc gcaagatcag cagccccgcc gagcggatca
     4801 aagatcagac caccgtcgcg atggatttcg gccgcattac gatcgacgag gatctggtgc
     4861 tgtacttgtt cggcacgccc gggcagaagc gcttcgactt catgtgggag atcctgtccg
     4921 aaggcatgct gggcttcatc gtcgtcgtcg acagctctcg tccggaaaca ttccgcgaag
     4981 cgcgctctat cctcgacacc ttccggggtt atgcgctgac gccctacgtc gtggcggcca
     5041 acaagcagga ccacgaggac gcctggtctc ccgaagacct gcgcatcgcg ctcaagatca
     5101 acagcgatgt caaagtatta ccgtgcgtgg cgcgggatcg agagagcgtg aagagcgccc
     5161 tgctcgagct gctgtattcc atcttggacc tgatggacag tgaaggggtc tagcttgacc
     5221 ccccctctcc cgccccccgt cagaacgggg ggagccggag gggggcgggg cgcactctct
     5281 cgccatgagc tcgatcgtca cctcgcaagg agtcgttcat tacgaagcct acggccgcgg
     5341 gcgaccggtg atcctgctgc acggctggat caattcgtgg ggcgtctggc aagaagagat
     5401 gatccagctc gccgatcgcg gcggctacaa ggtgtacgcc ctggatttct ggggattcgg
     5461 cgaatcggcc aaatcgaaga cgcctccgtt caagatcgca tcctacgtcg acatggtcga
     5521 ccagttcatg gagacgctcg gtatttttcg cgcgccggtt ctgggccact ccatgggcgg
     5581 cacggtggcc ctacgtttgg cgctggatcg gcccgaacgg gtgactcgcg tcgcgtgcgt
     5641 cggatcgcct atccacggga attcgctgaa tttctatctg aagctggccg gcatcgactg
     5701 gatcgcccac acgatgtggc gcgcgccggt tttgctgcgg ctcatgattc tgatcaacac
     5761 gcggggtgat tctgagcgcg tgcgcaagat gattctgcga gacgtcgaga aggccagcat
     5821 cgaatcgttc tttcgcagca tcggcgatct gcactcgacc gacttgcgcc agccgctgcg
     5881 ccggctggcg atcccggcgc tgggcgtcta cggcaagtcc gacaacatcg tcaacccgga
     5941 tcaggcgcgc gtcttggccg agacggtcca cgacgcgcgg gtgcacgttc tgggtcgctc
     6001 gcggcacttt gtgatgctgg atgaaccaga agcgttccac gggatcattt cggagtttct
     6061 ccgcaattag gatgcgcaaa tacattatcg aagacactcg cctcatctcg cccttcaacg
     6121 agaaggcgcg tgatttgaga atcctgaata agccgttgtg gctggcgcag cgcgatgcgc
     6181 tggcgtctta cacgcgtgag gaacgcccca ccgtccggtc gctcgaggaa gtgcccaacc
     6241 atcgcgacga agatcgcgtc gagacgatcg tctaccgcga caacctgttc ttcgacgcgc
     6301 cgtttatgga cgagttcatc cgccgcgcgc gcgagcagca caaggcgtgc cgcgtcgcct
     6361 tcgccctgaa cgacaaggcg atcacgacgc acgcgctcaa gctgcagtcg ggcattcacc
     6421 gcgaaggcga cgcgtacctg gccgatctct ggtatttccc gtacgggcgc gagagcaacg
     6481 tccgcccgct gctgatcgac acgcaggcgc gcgaaatcgg gtactacaat attcccaagt
     6541 acatcgcgcc gaaccagggc gatttgacct tccaggtgcc gcagcgcgcc tttctgtcga
     6601 tcgagcattg gtcgcacgtc tacgtcgcca actcgccgtt cggcgtgttc gccgagggcg
     6661 cgcgcatgga gaagcagatc gagcggctca gcttcaagct gcgcgtcctg tggcgcggcc
     6721 tggtcgagcg gcggcaggtg ctgaccaccc gcgcgctggt caagaagggc cgcaacgtgc
     6781 acatcgaccc gtcggccgtg atcctggggc cggtcacgct gggcgacaat gtcgtcatcc
     6841 agccgggcgc cgtcatcgac aactgtcaca tcggcaataa cgtgaccgta tcgcatgggt
     6901 gtgtgttgat gttgagcgtc gtgggtgacg ggtgcttctt gccgtggcgc gcttcgctgt
     6961 tcctgacgac gctcatggag aactcgatgg tggcgcagaa cacctgcctg cagatgtgcg
     7021 tggtcgggcg cgacaccttc atcggagccg gcaacacgtt caccgacttc aatctgatct
     7081 ccaagccgat tcgggtcatg catcgcgggc gcctcgaaga ggcgggcatg cccgtcatcg
     7141 gcggctgcgt gggccacaac tgccgcatcg gctccgggtt cgtcatttac cccggccgcg
     7201 agatcgagtc cgacgtgatg ctgacggcgg atcgcgccga gcgcgtgatc gtcaagaaca
     7261 tcacgtatcg acagagcgat caccatgaga ttccgggggc cgatcggctg taccctgcgc
     7321 agtaccggcc gacggcgtga tggacaattt cgctttcatc atccacccga tcgatccgaa
     7381 gcgcgacgtg cagcgcaaat tcccggcgct cggcaaagtt ctgcccgtgc cagccatcga
     7441 cttcttctcg cgcttcttcc cgccggtata catttcgcac gtcaccggcg cgcggtcggc
     7501 tgcacatggc cgtgaaatcg aagggtggct cgtggcctgc ccgttcacgc cgcagcgcat
     7561 gatgacgctg cccgccgaga cggtctacgc taagatcgtg aagactgccc gtctggcgca
     7621 gagcaggggc gcgcggctcg tgggattggg cgcgtacaca tcggtcgtgg gcgacgccgg
     7681 aatcacgatc agccggcggg tggatgtgcc ggtcacgacc ggcgactcgt acaccgtcgc
     7741 gatggctgac gcggccgtgc gcgaggcggc gcggcgcatg gagatcgacc tgggtcgcgc
     7801 gacggttgcc gtggtcggcg cgacgggcgc cattggcgcg gtcggcgcgg agctgcttgc
     7861 ccggcgagcg ccgcaattga tcttgatcgg caagcgcgcc gatcgactgc tccgcgtcaa
     7921 ggctcgctgc gaggcggccg gcgcggatgt ccaggtcgcc gcggacgtcg ccgccatccg
     7981 gcaggcggat gtgatcctca ccgtatcgtc ggcgggcgac gcgatcatcg cgccgcatca
     8041 cttgaagccg ggcgccgtcg tgtgcgatgt ggcccgccca cgcgacgtgt cacgcagggt
     8101 cgtcgaggag cgcggcgatg tgctggtcgt cgacggcggc atcgtcgaag tgcccggcga
     8161 ggtggatttc cacttcgact ttggcctccc gccgaggatg gcgttcgcgt gcatagccga
     8221 gacgatggcg ctggccatgg aggggcgcta cgagtcctat acgctgggca aggacctcac
     8281 actctcgcag gtggaagcga tcgacgatat cggtcgccgg cacggcttca ggctggctgg
     8341 atttcgttcg tttgagcgcg cagtgacgga cgagcagatc gagcatatca agcgcaacgc
     8401 gcacgtggcc cgccccccct ccggctcccc ccgttctgac ggggggaggg agaggcgggt
     8461 ggcgcgagct tgacggaggc cgtcatgggc aaaggtcgca tactggtcgt tgaggacgat
     8521 accgacattt cgaacatgct gcgcatctac ttcacgtcgc aaggctacga ggtcgtggtc
     8581 gccgggcgcg gcggcgaagc gctggagcgc tgtcgacagc aattgcccaa cgtgatcgtg
     8641 ctggacatca tgcttccgga catcgacggc tacgaggtct gcacgcagtt gcgcaccaac
     8701 ctgcgcacca gccacgttcc catcatcttc ctgacccaga aagatgagcg cagcgatcgg
     8761 atcgccgggc ttgagctggg cgcggacgat tacatcacaa agcccttcga catcgaggaa
     8821 ctgcgcctgc gtgtgcaggg cgcgatgcgg cgcgccgagc gcgagaacct gaccaacccg
     8881 acgaccggcc tgccgtccgg ccgcctgaac gaagagcagt tgcgcctgct gatgcgcaag
     8941 caggattggg gattgctgta cattgggatc gaggggctgg aggatttcaa caacgcctac
     9001 ggcttcctgg ccggcgacga cgtcctgcgc ttcacggcgc tgctgctagg cgagacgttg
     9061 gacgaagggg gcgcccccga cgactacatc ggccatgtcg gcggcgacaa gttcatggtc
     9121 atcaccaaaa tggaccgcgc ccggacgctg gccgacgcgc tgaaggcgcg atttgccaac
     9181 ggtatccttt cgcactattc gttcatcgat cgagatcgcg gctacgtcgt cattagagat
     9241 ccggacggca aggatcgcca ggcgccgctg atgcgaatgt gcgtcggcct gatccgatcc
     9301 gacgcgcagg agttcaccga catacgcgag attaccgagg cggcagcgga ggcgcgacgc
     9361 gtggattgcg caagcctgcc ctcgctaggc gcgggttcac cccctgaaac ctgaacacct
     9421 gataactgac acctgattac tgattactga caactgatta ctgataactg ataactgatt
     9481 atgacgaagc gtattggatt ccgcgggtcg atacaagtca agctttggcg gctgttcgtc
     9541 accggcatgg ctgtggcagg catgctgata gccgccatcg ttctcatcaa cagcggcgcg
     9601 atgacatcgg atccgctgcg ggcgatcctc ggcatcggcg ttttgatcgc cgccgtcgcc
     9661 accatttcac tcgccggcgt tttcgtccgc gcgcgcatcg tcgctccgat cgagtcgctg
     9721 cgggagggcg cggcggcctt tgcggcgggg aacctcgcgt atcgcatcca caccgacacg
     9781 ggagacgagc tacagtcgct ggcccaggaa atcaacgaca tgtcggctgc ccttcagcag
     9841 tcgcaggcgc gcctggcggc catggctcgc gagcgcgagc gtgaggccga attcgcccac
     9901 gctcgcatgc gcgagatgac cacactcatc cgctccggac gcgccattac gtcgctcgac
     9961 ctcaaggacg tgctcagccg cctggcgtac gaggcggccc gcgccgtgca gggcgatcgc
    10021 tgctcgatct acgtcctcga cagtcggcag cgacgcctgg tgctgcgcgg cgagtgggaa
    10081 gccacccccc tcttccctcc ccccgttctg actgggggag ccggagggag gcccgctggg
    10141 aatggtactg atcgcttctc caactctaac tctaactcca actctaacct ccaagctgtt
    10201 gtgggggatg tcgcctacga atggggggag ggcgtcgtcg gctgggtggc cctggccgag
    10261 aagcccatct tcctcgccaa tgcgcaggcg gacaagcgct tcgtcgccaa ggcgcccaac
    10321 gacggtgaga tcgccgccct tatcggcgtc cccgtgcact cggatagtgc cgtcgtcggc
    10381 gtgctccagg ccagcacgcg tcccggcacg ccggcattcg acgtgtcgga ccagcgcctg
    10441 ctgtctacct tcgccgcgca ggccgctgcc gccatcaaga actcctacct gtacgagatc
    10501 gagcgccgcc gcgccaagga aatgtcgatc atcgccgaaa tcatccgcgc catctcgtcg
    10561 tcgctcgacc tcgacgccac cctgaactcg atcctgtcct ccgtgcgcga gctggtctca
    10621 tacgatctgg ccgagatcac cctgtgggat cctcaggccg aagtcctgcg cacacgcggg
    10681 cgcggggccg acccgaagta cgacgagtac agccggacgg cgggtggcgt gtaccacctc
    10741 ggccagggca tcaccggctg gatggccggc catcgcaagc ccctgctttt gcccgacctg
    10801 gccgtcagcg aagtgcgccc cgccgtcgat ctcgagcaat tccccatccg ctccgtcgtc
    10861 ggcgctccga tgatctccgc gcagcagctc gtcggcacga tcgagctggc ctcgtatacc
    10921 gcccacgcct tcaacgaatc gcacctcgac accctgcgca cgatcgctgc gcaagcggcc
    10981 gtcgccattc aaaacgcccg cctctttatc gagtcgcggc agcgcgccga ggaagccgcg
    11041 ggcttgttcc gcgttgccag catcgccgcc tcggcgctgg agccgaacga gatcctcaaa
    11101 cagatcatgg ccgaagcggc gcagttgatg ggcacgcagc tcggcgtcgt catgctctac
    11161 cgtcccgaga cgcagcggct cgaggcgcat cccacggcgc tgttcggcgc gccggtcgag
    11221 cgcgtcctgg acatgaagat cgacacccgc aggccggcat tcctccacag cgtgttccgc
    11281 tcgggacggg tgtttcgctc cgacgacgca ccccgcgacc ggcgcattct cagggaatac
    11341 cgccccttcg tcgagcgctt cggcgcccgc cgcacactga gcgcgcccct cgtcgtccgc
    11401 gacactccca tcggcgaggt gcacatggcg cgcgccgccg gcgccccctt cgacctggac
    11461 gacgagcagc gcctgatgac cctggccaca ctcctggccg gcgtgatcga gacgaaccgg
    11521 ctggcgatcg agcgccagga gcgcctcgac gagctgcagg gcctggtcga gatctcccag
    11581 gcgctctccg cgctgaccga tgcgtcgacc gataagactc taactctaac tccaactcca
    11641 actctaactc caactctaac tcctttgcag caggtctact cgcagatcac gcgcagcatc
    11701 gccgagcgca tccgcgttca ggtcgcgggc ctgctcctgt tcgatccccg gcgcgagatg
    11761 ctggtgagcc agccgccttt ctacggcgtg cccgacgacg ccctcgagca ctacgtcatt
    11821 cccgtcacgc aaggcagcgg gctgcaccgc ctgtggacgg aggaggacac ctggatcagc
    11881 aacgacgtgc cgaacgaccc gctgaccagg gaagccggcc tggacaggct ggcgcgcacg
    11941 ctcggggtcg agcgggcctt gttcgccgcc atgaagatcg gcacgcggcg catcggcgtc
    12001 atccaggtca gcaacaagca cgacggccag cccttcggcg aagaggatgc ccggctcatg
    12061 tcgatctacg cgacacacat cgcggccgtc gtcgagaacg cccgcctgta cgcgcagacc
    12121 gatgtgcgct tgcagcagcg cgtcgaggag ttgacggcgc tgtccaacat cagccaggag
    12181 ctgaacgcga ccctcgatca ggagcgcatc ctggatctgg tgctcaacga ggcggggcgc
    12241 tccacgggcg cgacgcgcgg ggccgtcgcc ctcgtccccc cctccgccca ccccccgtca
    12301 gaacgggggg agccggaggg ggggcaggcc gagctggttc tggctgccat gctgggctat
    12361 acgccggagg agatcgagcg cgcgcgcctg ctggctcaac gcgtgggcca gggcatcatc
    12421 ggcgccgtga tcgagatggg cgcctcgatc gtcgtggacg atgtgcgcag gaaccctgat
    12481 taccttcaag tctcagagca gacgcgatcg gagctgtgcg tgcccattcg ctacgcgctc
    12541 gaggtcgtcg gcgccatcaa cctggaaagc cccagcgtcg gatatttcac gcgcgatcac
    12601 gtggccttcc tcaaggcgct cgcggcgcac gcggccatag ccatcggcaa cacccagcgc
    12661 tacgaggagc agttgcggcg cggcgatctg ctccggcggc gcgccgagca gttggcgaac
    12721 ctcttcgaga tcgggcaggc cttccgcagt gacaggccgc tgctcgaggt gctggaggac
    12781 gtcgtgcatg ccgtccagca aacagccggt ttcaacatcg tccttttgag tgtcctggag
    12841 ggcgacccgc cggcgctcga gcgcgtggcc gccgccggct tgcccgtggc ccgcctggaa
    12901 gagatgaaga aggtgcgcac cccgtgggag tccgttggcg ccatcatgca cgatgccttc
    12961 cgtatcagcc aatcgtacta cgtcccggcc gagcacaccg ccgtcaccag ccagttggac
    13021 acctggccgc ctttcaaggc cgacatcgtg ccgcgcgcct ccgggcgctg gcacgagcag
    13081 gacctcctct tcactcccct gcgcgcctcg ggcgatcgca tcctgggtat catatccgtc
    13141 gacgagccgt tcgaggggcg cgtccccgat cgggcgacga tcgaaacgct ggagctgttc
    13201 gccaaccagg ccgccatcgc catcgagaac gcgcggttgc tcgaagacct ccagcagcgt
    13261 atcgacagcc tgaccctctt caatcaggtc agccgcacca tcagcgcgcg cttggacctg
    13321 gatgggttgc tggcgacgat cgtcgacgcc agcatcgaat tggccaaggc gaacaccgcg
    13381 acgattttca tacgcgacga cgccggcgtc ttccggccgc gcaagtctca cggctggcgg
    13441 cccgagcaga tcgcgcacct gaattgggcc gagggcgagg gcctcgtcgg caaggtcgcg
    13501 cgcgatgggc acgccttgat cgtgccagat gccaaagccg atccgctgtt tacgcccgac
    13561 ccgacggacg gcgcgatcgg ggccatgctc ctggtgccga tcgcggtggg tggcacggtc
    13621 atcggcgtgt tgagcgtgga caagcccttg ccgcgctctt tctcgaacac cgatctgctc
    13681 atgctctcca ccctcagcga tcaggctgcc atcgccatcg ccaactcccg cctcttcgac
    13741 gaggtgcgcc gcttctcctt cgagctggaa gagcgcgtgc gccagcgcac agaggagctg
    13801 gctcgggcca acgccgacct gacgctggag cgcgaccgcg tcgagacgct ctaccgcatc
    13861 acgtccgagc tgtccatgag cctcgacctg gatcgcgtcc tcaaccgcgc cctggcgctc
    13921 gtgaacgaag cagtcggcac ccggcgcagc tccattctgc tggtcgatca gaacagcgat
    13981 cggttgattc accgggccgc catgggccgg ggcgagtacc tgccgcccgg tggccggccg
    14041 acgcgcttcc gccgcgacga gggcctggcg ggctgggtta tccgcaatcg catgcccgcc
    14101 atcgtcgccg acatccgcgt cgacgaccgc tggctggagc cctcaccccc ggcccctctc
    14161 ccgttgcaga cgacaccgga gccctcaccc ccagcccctc tcccgtcgcg ggcgacagga
    14221 gaggggagcg caagcagccg tgagtatcga tcggcgctgg ccgtgcccct gatcgccggg
    14281 gacgacgcgc tgggggcgct gcttttgctg cacccggagg ccggctactt cgaggagtcg
    14341 cacttgcgcc tggtggaggc ggccgccacg caggcggcga cggccatcaa caacgcggca
    14401 ctgtacggat acatacgcga gagcgccgag cgcctgggcg ccatgctccg cgacaagcag
    14461 atcgaggcgg ccaaggccca ggccatcctc gaatcggtgg cggacggcgt gctcgtgtcg
    14521 gacgcttcgg agacggtcat cctgttcaac gccgccgcgg agcgcatcct agggcgcgcg
    14581 cgggagacgg tgcttaaccg gccgacggcg gacatcatcg gcctgtacgg ggcgtcgggc
    14641 gcgcagtggg aggagcagat tcagcgctgg cggagtggcc cggagtcgcg gcgcagcgtg
    14701 ccctcgcttg ccaccaacgt gcatttcgag gacgagaacc gccatgtgcg cgtgtccatc
    14761 gccccggtca ccggtccggg cgacgagtta ctgggcaccg tgtccgtcgt gcgcgacatc
    14821 acggccgagg ttgaggcgga tcggaccacg cgcgatttcg tgtcgaacgt gagccacgag
    14881 ctgcgcacgc cgatgaccag catcaagggc tacgccgacc tgctgctgat gggcgctgcc
    14941 gggtcgctca acgaaaacca ggagcgtttc ctgaatatca tccgctccaa cgcgaagcgt
    15001 ttggagacgc tcgtcgccga cctgctcgac atcaaccgca tcgatagcgg gcgcgccatg
    15061 ctcgacatca agcaggtgag catggtcgcc gtcctcgagc aggtgctgac ttcgctacac
    15121 gagcgcatcg agcagaagag gctgtcgatc aagacggagc tgcccgaggg cgattctctc
    15181 atcgtgctcg gcgatcaggc acgccttctg caggtcttga cgaacctcgt cagcaactcg
    15241 caccaataca c
//
LOCUS       DBNHXB010000159        20495 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2217096, whole genome shotgun sequence.
ACCESSION   DBNHXB010000159 DBNHXB010000000
VERSION     DBNHXB010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 20495)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20495)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20495
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2217096"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..1924)
                     /locus_tag="AC162G_06175"
     CDS             complement(278..1924)
                     /locus_tag="AC162G_06175"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HIR5075432.1"
                     /translation="MSLKIRPPRPLPDDVAKLGQLLLPTGSPYRYVGDDLYPQLNEAD
                     WADMYPAEGQPAESPVDLMLVTAFQYLEHLPDRAAAEAVRLRIDWKYALHLPLNDTGF
                     NFSVLSEFRDRLLAHAAERRLFDHMVARLQQAGLIKTRGRQRTDSLSVLTKVRDLSRL
                     ELVLETLRLAVRAIVAAEREWACAHLPFSWEQQYGSRAIEYQMSDAQRCELEHSVGRD
                     GFWLLDRLDARETPAGLRDLEAVHILRRVWAQKFVRDESGVVWRPGGRYDGHTEIHTP
                     HDPEARWSAKRGRGTVGYRLQVTDTDEDGVPHLITDIALTPHTQADATALPEIQARLV
                     ERGVAPGTHLTDTAYFSGANAQTSAQAGIDLFSPAPLDASPQAHLPEGLTNDQFLFDW
                     DKRQVTCPGGQVSSRWHEQPGAADGEVVVQAVFAREVCAACPLRPRCVVGDLKRDGRK
                     VQRSSTWPELHAQRQRQRTPAFQQEYKRRAGVEGTLSVLVRQQGARAMRYLGTAKGHL
                     QALFTGVAFNLKQAAAWLAGRRPQRRRRGLGLAAGSLDTG"
     gene            2859..3296
                     /locus_tag="AC162G_06180"
     CDS             2859..3296
                     /locus_tag="AC162G_06180"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR5075433.1"
                     /translation="MSTEQNKALVRQMVEEVFNRGNVSLADEFLAPDFVEREELPPGI
                     PRDREGVKQLTTMLRNAFPDFKATIDDIVAEGDKVVIRQTWCGTHKGEFMGVPPTGKS
                     VSFGVIDIIRIAGGKFVEHWGQMDSMGLMQQLGAVPTPGQGGS"
     gene            3523..3945
                     /locus_tag="AC162G_06185"
     CDS             3523..3945
                     /locus_tag="AC162G_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5075434.1"
                     /translation="MSSVTEADLRAQESRLLTAMLQNDATVLSSLFDREYVFTSAGGA
                     VWDRDKALQDFADPTYSVGKLDVELERVIPLTDAGVVVGRSRVAGRARGVSVSGVYRF
                     TRVWRRNGDRWTILATHTSVAEPPADEVGGSRSCRTRG"
     gene            4202..4546
                     /locus_tag="AC162G_06190"
     CDS             4202..4546
                     /locus_tag="AC162G_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075435.1"
                     /translation="MEIERNVTSIMLRNVSLSLVFLAMLQIAEFVQSGFLFQHIALCV
                     VCIILSVMAGRISVTFQTWFYSGVFEAIAARSLQVSDLMTVKRASAPVKEGVEELIAP
                     PNPRMQPVTVQC"
     gene            complement(4672..6948)
                     /locus_tag="AC162G_06195"
     CDS             complement(4672..6948)
                     /locus_tag="AC162G_06195"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR5075436.1"
                     /translation="MLELKDFFHLPEVDVLLAQVVADSRGITVVAGRGSRLAQVGSAG
                     AVESSMAQSGRSTIAAILLRQILDRPASDRPVQAVVVAPDRGAVRVANHQRKQVEFVP
                     APIDQVDGFTTGLQRAVMRNPDLLVVDRLNTVTAQAVFHAAAIGRRVLTQIDTALVGG
                     EVLQEIVGLGCSPSQTSVVSWVVAVQRVPALCTACKMPHRPGAPLLDVMAERHGIDRD
                     VTYYAASGCENCGGTGYFGELTAFDIFRVHGGVTSFSELVSTPSLLPLEGYLARLAER
                     GYVSLEDAARAGPSTLRHVQQMLSVREDTLDETKRALERKLAEVEAANKVLAQRTEAL
                     IALQDVSHALSASTELDDVARRVVHYARELCGADRAVLYLVEPPDDGRVQAVEGWDAS
                     AVGRTARLPASMVERDGPGSVVGAPPAIDLPPEQVRALQAGLRVPLVAEDTLVGLLIV
                     NSLYKTRFAPGSVALLQTLGSHAAVSIQRVRLFEALRDKIAQLEEARAELITKERLDR
                     ELELARDLQLRLLPDRFPATDGVQFAAAYRPAREVGGDLYDVIDLGQGQLGLLIADVS
                     DKGLPAAFYMGMVRSLILSEARRTDSPREVLSNVNRILRQASRADMFLTAFYGIYDVA
                     ARRLAYCRAGHDQPVWLRTDGHAVRLGAPGMLLGMIDPEELDLAEVEVFLSPGDTLVL
                     FTDGMTDALDPYGVEFGVAQWVSRAWENAGLPVERLCAMLFDEVSAFQSTAPQMDDMT
                     LLVMRVCDFPFEEELGCG"
     gene            7088..7372
                     /locus_tag="AC162G_06200"
     CDS             7088..7372
                     /locus_tag="AC162G_06200"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR5075437.1"
                     /translation="MKQASESLHATVHGRVQGVNFRYYTQLKAQALNLTGWVRNRPDG
                     TVEVVAEGARWALDQLLDFLYHGPSHAHVDRVDVEWLKATGQFTHFQVTG"
     gene            7373..8620
                     /locus_tag="AC162G_06205"
     CDS             7373..8620
                     /locus_tag="AC162G_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075438.1"
                     /translation="MNHNPERLAWTIMLLSFGMCVALAISVPLGIRSFLTDSTDPAPL
                     VLDVQQGAVLLKRPAFGDFILVPSQTTDVPDGSIIRADQATQAILTMRDPDLVTNRVV
                     VQLYANTEVTLERANSPRFGVSPNPHRLDLFVTSGRVRINVLNGTNRGVTATARSPQG
                     EVTFTTGTYALEVTNEELQITAREGQATVSAQDSSVTIEPSQRAVVRLGQPPVGGLAD
                     ERPLIVNGSFAAPLESAWQIEHVPLGSPEPTGSVITTTIVGRHAAVFERTGPSHAETR
                     LAQDLNRDVTDAASLTLHFAVLVNSQDVPVCGSVGTECPMMVKIDYRDTSGANREWLR
                     GFYAVNDPNNINKPFCGSCTRPFEHQRVLSNIWVSYDSGNLIDVLTTPDGFKPTRITR
                     ITFYASGHSYRSAITDVELLMQD"
     gene            complement(8682..9380)
                     /gene="radC"
                     /locus_tag="AC162G_06210"
     CDS             complement(8682..9380)
                     /gene="radC"
                     /locus_tag="AC162G_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR5075439.1"
                     /translation="MEEVEYGSTIKDMPSGDRPRERLAHAGASALSNAELLAIILRVG
                     TGGENAINLAQRLLNKHGLAGLARRPVAELSQEKGLGQAKVVQMKAAFELGRRLLAST
                     PEERPQIRTPADAANILLSEMALLEQEHLRVMLLDTRNRVLATPTVYVGSLNTTWVRV
                     SEVFRDAIRQNAAALIVAHNHPSGDPSPSPEDVTLTREIINAGKLLDIEVLDHLIIGH
                     QRFVSLKERGLAFE"
     gene            9504..10019
                     /locus_tag="AC162G_06215"
     CDS             9504..10019
                     /locus_tag="AC162G_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR5075440.1"
                     /translation="MDFQSIVLAFLAFSGAFLAALWLALIIWTFRDMRSRSRDIFAQI
                     LAALLVAVLTLPGLLIYLILRPKETLAEAYERSLEEEALLQSIEDVEHCPGCGSRVHS
                     DYLVCPSCNTKLKKACRSCGRAINLRWNVCPYCATPQISSVAPVQATTAETAETTQRM
                     AVRREESSEVA"
     gene            complement(10054..11466)
                     /gene="pyk"
                     /locus_tag="AC162G_06220"
     CDS             complement(10054..11466)
                     /gene="pyk"
                     /locus_tag="AC162G_06220"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR5075441.1"
                     /translation="MARTKIVCTIGPTSSQVEMLRVMIRAGMSVARINFSHGDRTAHM
                     ARIAAIRQAALAENAVVAILGDLQGPKLRVGAIRGDYVMTRIGDEIALTTRDVPGDRG
                     EVHLPHAELIREARPGQRLLLDDGQLEFVVLETTGTDMRCRVVTGGPLGSYKGVAAPG
                     ADLSLSALTDKDRDDTRFAVEQGLDFLALSFVRSDRDVRQLRDWLANLDARIPIVAKI
                     EKPEAIAAFDDILAGSDAVMIARGDLGIEIPAERVPFVQKDLIARCNLAGVPVITATQ
                     MLNSMVDRPRPTRAEASDVANAILDGTSAVMLSAETATGEYPLETVQTMARIAEAAEA
                     HLPYEETIRRARLERDPSPAAAISRATVEIAYELGAKAIVTSTYTGSTARWVASYRPR
                     TPVLAITPRQEVQRQLALAWGVRPVLVPYYETTDDMLAYAAQLAEESGLAGRGDTIVI
                     TAGIPAGERYRTNMLKVHVI"
     gene            complement(11567..12415)
                     /locus_tag="AC162G_06225"
     CDS             complement(11567..12415)
                     /locus_tag="AC162G_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR5075442.1"
                     /translation="MLKIISDTTTGLTKGVAAQHGVDLVPQIVMFGSETLRDGVDITN
                     DVFLQRLKASKAFPTTSQPSVGDFLRIFKPLLDAGHEILCITVSSYLSGTYNSAETAK
                     KELGSDRITLVDSKTVAAAEALLVLEAAHMTRDGKSAPEIASRLDALIPSTTLDFVLD
                     TVEYLVKGGRATRIQGIIGTMLQLKPVFSIRNGTGHIEVLERIRTRNKALARLRGIVD
                     EALHGKSKFQLAVTHTGLHGEAEALAHEFRGKYGLGECMVIEMPPAVAAHAGPGALGA
                     AYVVED"
     gene            complement(12520..13842)
                     /gene="thrC"
                     /locus_tag="AC162G_06230"
     CDS             complement(12520..13842)
                     /gene="thrC"
                     /locus_tag="AC162G_06230"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR5075443.1"
                     /translation="MNNVKGFRCTICGTEYDPGEVEYVCPKHGDAGVLDTVYDYRAIA
                     RQASPGQLANMQTSASLNMFRYNPLLPIDLESEGLPLQVGWTPLYRAPRLEAKLGLHE
                     VWLKDDGRNPTASFKDRASALVVVRAAQERRHIVTTASSGNAGAALAGMAASVKMPTV
                     IFVPQSAPQAKVAQLLIYGATVFLVRGNYDVAFELCLEASKAFGWYCRNTAYNPFTVE
                     GKKTAALEICEQLTLSKLQPPASNFQIWDAPDRIFVSVGDGNIISGLHRGLVDLAELR
                     WIDRMPKLMGVQAEGAAACYRAWSAGADDVSAVSAHTIADSISVDLPRDGRRAIRAVR
                     QTGGAFVTVSDEDILAAMRELAQEAAVFAEPAAAAAYAGLASAVQAGQIGPDERVVVL
                     LTGSGLKDVPAAVKAAPSALVIDPHLDAVRTVLDALSGRRPPGAGGET"
     gene            complement(13839..14663)
                     /locus_tag="AC162G_06235"
     CDS             complement(13839..14663)
                     /locus_tag="AC162G_06235"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR5075444.1"
                     /translation="MKDERRTRHNGALRVATLNILVDLCRWRERAPLIVEEFRAIQPD
                     LIALQEVALPADNAQWIADQLGGYSVHLCPKTGAKGQREALGILSRLPVEEHRALPLV
                     AQDRVAQRVAVRHNGAPFVLVNTHLYWNPLDDAPRLEQARRILDWIPNHTPAVVCGDF
                     NAEPHFKSIAAMKARFASAHALAHGQEPEYTCPTPLARGRRMDAALWRMLERLSGLLL
                     KGTNDSWRGTLDYIFVDGAVRVERCEVAFNRPSPDDERLYPSDHFGLVADIRMEPK"
     gene            complement(14660..15067)
                     /gene="cdd"
                     /locus_tag="AC162G_06240"
     CDS             complement(14660..15067)
                     /gene="cdd"
                     /locus_tag="AC162G_06240"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006694821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIR5075445.1"
                     /translation="MPDDIQSVLIARAMAARAQAYAPYSNYRVGAALLAVSGKIYDGA
                     NVENAVYPVGICAERTALVKAVSEGERRFEAIAVATHNGGSPCGACRQMLSEFGLDLK
                     VFLVDGAGMVVHETTLHELLPRAFGAADLKVKG"
     gene            complement(15196..15612)
                     /locus_tag="AC162G_06245"
     CDS             complement(15196..15612)
                     /locus_tag="AC162G_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR5075446.1"
                     /translation="MVITKQLTLQTRGDGDTHDLTHAVARAVEESGARSGVVTLFVVG
                     STAGLTTIEFEPGAVADLQTAFERVAPRDGVYQHHLRWGDDNGHSHVRAALLGPSLSV
                     PFADGRLALGTWQQIVLVDFDTRPRRREVVAQIVGE"
     gene            15662..16213
                     /locus_tag="AC162G_06250"
     CDS             15662..16213
                     /locus_tag="AC162G_06250"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002645886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR5075447.1"
                     /translation="MGTQRAATPGTRYNRITMVIGDYHIRVEKDYTVFSAAHFITYDG
                     HQCEQLHGHNYRAAAALAGDLDENAYVFNFVPLKRALKGVVDQLDHKTLLPQDNPRLQ
                     VRIGDESVEVAYPSKHKRYVFPRGDVAVLPIPNTTAEMLAAWICGQLRQALRAYDTRG
                     IRSIEVEVEESPGQRAIYREEWN"
     gene            16215..17720
                     /gene="mptA"
                     /locus_tag="AC162G_06255"
     CDS             16215..17720
                     /gene="mptA"
                     /locus_tag="AC162G_06255"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HIR5075448.1"
                     /translation="MSEPHTVYFSLGSNLGDRQSNLIEAIQSLRANVQIERVSSVYES
                     RPAHAADQPMFLHVAVKGTTDLEPAGVIRFIQRIERRLGRVRPDRHVPCPIDIDLLSY
                     DDHVVSSDGLTLPHPSLAGHAHVLAPLDEIAPEWKHPTLKRSARQLLDDSGGSDGVKR
                     LAHGLTARPDRDVQSEEPPALLRLDRVGVTGLQRVLRLSTVKGSLYHARIDLSVELGP
                     EQKGAHMSRFSDTLEEVIDEIGGTEAATVESLAERIARQVVSDQRARRADAEIHAHFP
                     MYKHAPLSGKRTQEIYTLIGIAAATPDRSVGVIGVEAEGMMACPCAQDMVRAAARERL
                     LEDGIDEAMADRVLDLVPLATHNQRGRGRLLVGLPYPMRPADPPAVRAQDLVEIVEAS
                     MSAESYDLLKRPDELFVVQKAHRHPRFVEDAVREMLNNFVEMYPDLPDGAYLSARQVN
                     LETIHKHDVFAERGGTLADLRSEIRGGSGRTLTLDAWLAGQLGESDLIDPA"
     gene            complement(17810..18946)
                     /locus_tag="AC162G_06260"
     CDS             complement(17810..18946)
                     /locus_tag="AC162G_06260"
                     /EC_number="1.13.11.52"
                     /inference="COORDINATES: protein motif:HMM:NF013402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0033754 - indoleamine 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indoleamine 2,3-dioxygenase"
                     /protein_id="HIR5075449.1"
                     /translation="MATVDLAAYHIDPVRGFLPAKDPLERLPAGFDAWERAAPDMPVL
                     LMTGRLRPTLEALPMLNIERLEDEMQLERAMLLLSVFGNAYVWAGPEPARKVPRAVAM
                     PWCRLAEKLGRPPIAAHPSTVLFNWRRLDKTGPIELDNLATLQLFLGGMDEQWFYLTA
                     VVIEAKGAPALGALVAAQQAVAGGRADDLAARLETIASALARVYDVLERIPEKCDPYI
                     FYHRVRPYQTGWLEPGVIYEGVSEAPRMCTGGSAAQSPLIQALDAGLGITHRSIETSP
                     FLMEMRNYMVTGHRRFIEALENGPSVRQFVLDRAAQCPALGDRYNACIRELDRFRKKH
                     MELAVRYISHQAPNPDEAKGTGGTHFASFLSKARKETWEHTIRE"
     gene            complement(18984..19682)
                     /locus_tag="AC162G_06265"
     CDS             complement(18984..19682)
                     /locus_tag="AC162G_06265"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5075450.1"
                     /translation="MAGSNLLLLHGALGAGDQFAPLWLPLADHYKLHLVDFEGHGRAP
                     GRGRPFRIEHFIQNVVEYLDTHGIGSTSIFGYSMGGYVACSLAAGHPQRVERVATLGT
                     KFFWDAGIAARETALLDPHKIAAKVPHYARALAERHTAAGWETVLKETSGSLWSLAER
                     GGFARDDLARIEQRVRIMVGDRDSTVGVAECVEVYRALPHGELEVLPGTPHPLERISH
                     DRLSRSLIEFFGSE"
     gene            complement(19693..20292)
                     /locus_tag="AC162G_06270"
                     /pseudo
     CDS             complement(19693..20292)
                     /locus_tag="AC162G_06270"
                     /inference="COORDINATES: protein motif:HMM:NF025589.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
BASE COUNT         3731 a         6752 c         6308 g         3704 t
ORIGIN      
        1 ttgacgctca tgagcctcgg actctacaac gaaccctcgg gatcgttctt gccttcgatc
       61 tactattgac gctttgatcc gctgatgttc cgcactgtgc ctgaacgcga ccgtgccggg
      121 tcatagagat actgttggcg aactaacgcc taacgctgag ggtgctatgc tcaacgcata
      181 ccgtccggcg ggttccaacg tcacggctgg tctggcgtgt ctgtctgagc ggtcgtgggc
      241 tggttttttc agcctcgcct cgacgctgta tcctcggtca gccggtatcg aggctcccgg
      301 cggccaagcc cagcccgcgg cgacgccgct ggggacgacg tcccgccagc caagcggcgg
      361 cctgcttgag attgaacgcc acacccgtga acagggcttg cagatgacct ttcgccgtgc
      421 cgaggtagcg catcgcgcgc gcaccctgtt gacgcaccag caccgacaaa gtgccttcca
      481 cccccgcgcg gcgtttgtat tcctgctgga aggctggggt gcgttgccgc tggcgctgag
      541 cgtgcagctc cggccaggtg ctcgatcgtt ggaccttgcg gccgtcgcgc ttcaagtcgc
      601 cgaccacgca acgggggcgc aaggggcagg cggcacacac ctcgcgcgcg aacaccgctt
      661 ggaccaccac ctcaccgtct gccgcaccgg gttgttcatg ccagcggctc gacacctgtc
      721 cgccgggaca ggtgacttgc cgcttgtccc aatcgaaaag gaattggtca ttggtcagac
      781 cctccggcag gtgcgcctgt ggcgaggcat cgaggggggc gggcgaaaac agatcgatgc
      841 ccgcctgcgc actggtttgg gcattcgccc cggagaagta agccgtgtcg gtcaggtgcg
      901 tcccgggcgc gaccccgcgc tccaccagac gggcttgaat ctccggcagc gccgtggcat
      961 cggcttgggt atggggcgtc agcgcgatgt ctgtgatcaa atgcgggacg ccgtcctcat
     1021 cggtatcggt cacctgcaag cgatagccga ccgtgccccg gccgcgcttg gccgaccaac
     1081 gcgcctcggg gtcgtgcggg gtgtgaatct ccgtgtggcc gtcgtagcgc cccccgggcc
     1141 gccagaccac gccgctctcg tcgcgcacaa acttctgcgc ccagacccgc cgcaagatgt
     1201 gcaccgcctc caggtcgcgc agcccagccg gggtctcccg cgcgtccagc cgatccaaca
     1261 accagaaccc atcccgcccg acgctgtgct ccagttcgca ccgctgggcg tccgacatct
     1321 ggtattcaat ggcgcgcgac ccatactgct gctcccacga aaagggcagg tgagcacacg
     1381 cccactcccg ctcggcggcc acgatggcgc gcaccgccag acgcaaggtc tccagcacca
     1441 actccaagcg gctcaagtcc cgcactttgg tcagcacgct caacgagtcc gtgcgttgcc
     1501 gcccccgcgt cttaatcaac ccggcctgct gcaaccgcgc caccatgtga tcaaacagcc
     1561 gtcgctcggc cgcgtgcgcc agcagccgat cccgaaactc actcagcacg ctaaagttga
     1621 agcccgtatc gttcagcggc aggtgcaagg catacttcca gtcgatgcgc aggcgtacgg
     1681 cctcggcggc ggcacggtcg ggcaagtgct ccaggtattg aaacgccgtc accagcatca
     1741 agtccactgg cgattcggcg ggctgccctt ccgccggata catgtcggcc cagtccgctt
     1801 cgttcaactg cgggtagagg tcatccccga catagcgata tgggctgccg gtcggcagca
     1861 acagctggcc cagcttagcc acgtcgtccg gcagcgggcg cggcgggcgg attttcaagg
     1921 acatcgctcc ttcctccagc acaggctggc ggatattcta ttctcaattc gccaacagta
     1981 tctcacagac ccagccgcac cgaaggtcga gggtcgcaga ccctcgacga gctttatagt
     2041 acacactgga tcattatacg gtctgaaccg tttgatctct cgctggccga cccgcacgcc
     2101 cggcggtgaa acgggacagc ctgaccaaga cgcccggcga cgaaagccgg gcgttttgct
     2161 tttggggctg catgggtgta gactttatgc caaactgccg tgggcggcgg ctgtcacgtg
     2221 ttccgtccgg gcggtcagcc gcaccggggt ggggttcaat agtagccact ttcaagctag
     2281 atataagcta ttatccaatc gtcggataat tgcccctatg gggacaatat acgacatgtt
     2341 cgttggataa tcccgagtga ggcgcctgcg tatccttcag gaatatcggc ttggtaacga
     2401 caatcaccga ggatttgtta acagctcacg acgaagtcgt cgcgctcatt caagaaatct
     2461 cagacgcagc gcttgactgg caacccagca gcgagaggta ggctttttcg gtggagtagg
     2521 ccagatgttt ggcctggagg aggtcacgcg ctcgttgccg cacgacgttg agtttcatgc
     2581 ctgacaccct gatggaattt gtgctaaagt ggtttcagcc tacttagtgt gcgcgataaa
     2641 ataattgtag catactaagt gtgcataatc aagagttagc ccgcccaggt cgcgccaatg
     2701 attgcgttta ccatcctgcc aatcatgttc ccacaatccg gtgcgacaga ggtggatgca
     2761 gagcacgagg cgatgaagga ggtgtgtcag taaatcgaat gaccaatctc ttgcgcgcag
     2821 tgtgaatcgt atcccataca aacaaaggag aataacccat gtcaactgaa cagaataaag
     2881 cgttagtccg tcaaatggtg gaggaagtct tcaatcgggg aaacgtgagt ctggccgacg
     2941 agttcttggc gcccgacttc gttgaacgtg aagaactccc acccggaata ccgcgtgatc
     3001 gtgagggcgt caaacagttg accaccatgt tgcgcaacgc cttcccagac ttcaaagcca
     3061 cgatagacga tattgtcgcc gaaggcgata aggtggtaat tcgtcagacc tggtgcggca
     3121 cccataaggg cgagtttatg ggcgttccgc cgaccggcaa gagcgtatcc tttggagtga
     3181 tcgacatcat ccgcattgct gggggcaagt ttgtggaaca ttggggccag atggatagca
     3241 tgggtctgat gcagcaactt ggcgcagtcc ccacgcccgg acaaggcgga agttagacac
     3301 tcggccggag ttgcaagcgg agcgcgtcac tttttcaaca tgggtgttac cagaggtgca
     3361 gacatacgaa gccaagcgag gggctttgct gtcgtagagt acgggctaac accgggttgc
     3421 aggtgacgtt gctccgctac gctccgcaac gcgcctgaac ccggccgttc ggcagactta
     3481 gagacgtcgg cggcgggagc gggatcgcgg caggagggcc ggatgagcag cgtaacggaa
     3541 gcagacctgc gcgcacagga atcgaggctg ctcacggcga tgctccaaaa cgatgcgacc
     3601 gtcctctctt cgctattcga tcgggagtac gtgtttacga gcgcgggcgg cgctgtctgg
     3661 gaccgcgaca aggctctgca ggactttgcg gatccgacct actcggtggg caagctcgat
     3721 gtcgaactcg agcgtgtcat tccgctgacc gatgcagggg ttgtggtggg gcggtcacgt
     3781 gtagctggca gggcgcgcgg agtctcagtc tcgggggtgt acaggttcac gcgggtgtgg
     3841 cgacgaaacg gtgaccgttg gacgatcctc gcaacgcaca cgtcggtggc tgagccgcca
     3901 gcggacgaag taggcgggtc tcgaagctgc cgaacaaggg gatgaagctg acgaagcttt
     3961 cggcggcacc gctgcacggt aggcgaggat ggttcgtact aaggtgccgc ctcatgcccg
     4021 cgccgccgcg gtcggacgcg ggcaccgctt cgcagcttat ccccggtgtt gggcggcggg
     4081 gtacacttcc aacatagaaa gaacaagagg ataagcggat gtcaatttgg aaatcgcttt
     4141 tcggcccgga gactggccta ttctgatggc ctatatcaag cgagataggg ttgaggtatc
     4201 ggtggaaatc gaacgcaatg ttacgagtat catgctccga aatgtaagcc tcagtcttgt
     4261 cttccttgct atgcttcaaa tcgccgaatt tgttcaaagc ggtttccttt tccaacatat
     4321 agcgctgtgt gtggtgtgca taatcttgtc ggtaatggct ggtagaataa gcgtcacgtt
     4381 tcaaacatgg ttctactcag gcgtatttga agccattgcg gctcgtagcc tgcaagtatc
     4441 agacttgatg actgtcaagc gtgccagtgc gccggttaaa gaaggagtag aggaattgat
     4501 tgcgccgccc aacccgcgca tgcagcctgt tactgtacaa tgttgaagga agatggcaga
     4561 atcaggccag cgcagacgag cccatcatcg cgctggcttc gcctgtcggt cttccgcctg
     4621 ggtgatgacc tgcttgtagc tgcgcccgga gcgctcccgt cgggtctgtg cttacccgca
     4681 ccccaactcc tcctcgaatg gaaagtcgca cacgcgcatg acgagcagcg tcatgtcgtc
     4741 catctgtggc gcggtcgatt ggaaggcgga cacttcgtcg aagagcatcg cgcacaggcg
     4801 ctcgaccggc aggccggcgt tttcccaggc gcgactgacc cactgcgcta cgccgaactc
     4861 gacgccgtac ggatcgagcg cgtccgtcat tccatcggtg aagaggacga gggtgtcgcc
     4921 cggcgagagg aatacctcga cctcggccaa atccagctcc tccggatcga tcatgccgag
     4981 cagcatcccc ggcgcgccca gccgcacggc gtggccatcg gttcgcagcc acacgggctg
     5041 atcgtgcccg gcgcggcaat aagccaggcg acgtgcggcc acgtcgtaga tgccataaaa
     5101 ggcagtgagg aacatgtcgg cgcggctggc ctggcgcagg atgcgattga cgttgctcaa
     5161 tacctcgcgc ggcgaatcgg tgcgccgggc ttccgagaga atcaaggacc ggaccatgcc
     5221 catgtaaaac gcagccggca agcccttatc cgacacgtcg gcgatcaaca ggcccaactg
     5281 cccctggccc aaatcgatca cgtcgtagag atcgccgccc acctcacgcg cgggccggta
     5341 ggccgcggcg aattgcacgc cgtcggtcgc cgggaaccgg tcgggcagga gacgaagctg
     5401 aaggtcgcga gccagctcaa gctcgcggtc gagtcgctcc ttcgtgatca gctcggccct
     5461 ggcctcctcg agctgcgcga tcttatcgcg cagcgcttcg aacaggcgca cgcgctgaat
     5521 cgacacggcc gcgtggctgc cgagcgtttg gagcagtgcg acgctgcccg gcgcaaagcg
     5581 cgtcttatac aggctgttga cgatgagcag gccgaccagg gtgtcttcgg ccacgagggg
     5641 cacgcgcagg ccggcctgca gggcgcgcac ttgctcgggc ggcaagtcga tagccggcgg
     5701 cgcgccgacc accgagccgg gaccgtcgcg ctccaccatc gacgccggca gtcgcgccgt
     5761 ccgccctacg gccgaggcgt cccatccctc gacggcctgc acgcggccat cgtccggcgg
     5821 ctcgacgaga tacagcaccg ccctgtcggc gccgcatagc tcgcgcgcgt agtggacgac
     5881 gcgccgggcg acatcgtcca gctcggtcga cgcgctgagc gcgtggctca cgtcttgcag
     5941 cgcgatcagc gcctcggtgc gctgagccaa caccttattc gccgcctcga cctcggccag
     6001 cttgcgctcg agcgcgcgct tcgtctcgtc gagcgtgtct tctcgcacac tgagcatctg
     6061 ctgcacgtgg cgcagcgtcg aggggcctgc gcgcgcggca tcttccaggg acacgtaacc
     6121 ccgctcggcc aggcgcgcca ggtatccttc gagagggagc aggctgggcg tcgacaccag
     6181 ctcggaaaag ctggtcacgc cgccgtgcac acgaaagatg tcgaacgccg tcagctcgcc
     6241 gaagtagcct gtgccgccgc agttttcgca acccgaagcc gcgtagtacg tcacgtcgcg
     6301 atcgatgccg tgacgctcgg ccatcacgtc gagcagcggc gcgcctggcc gatggggcat
     6361 cttgcaggcc gtgcacagcg ccgggacgcg ttggacggca accacccagg acacgaccga
     6421 cgtctggctg gggctgcagc ccaggccgac gatctcttgc agcacctcgc cgccgacgag
     6481 cgccgtatcg atctgcgtca ggacgcgccg cccgatcgcc gctgcgtgga acaccgcctg
     6541 cgccgtgacg gtattcaatc gatcgacgac gagcagatcg gggttgcgca tcaccgcgcg
     6601 ctgcaggccg gtggtgaagc cgtccacctg atcgataggc gccggcacga actcgacctg
     6661 tttgcgctgg tgattggcga cgcgcactgc acctcgatcg ggggcgacga ccacggcctg
     6721 cacaggccgg tcgctggcag ggcgatcgag gatctgccgg aggagaatgg ccgcgatggt
     6781 cgaacggccg gactgcgcca tcgacgattc gacggcgccc gccgaaccga cttgcgccaa
     6841 gcgcgagccg cgcccggcga cgacggtgat gccgcgcgaa tccgccacca cctgcgccag
     6901 cagcacgtcc acttcgggga ggtgaaagaa atccttaagc tccaacatgg caggcgactc
     6961 gattataccc acgtggccca aagcgtgcca ataggacaac cggctcgctt tacgccttcc
     7021 cgcgccgatc ttccttgacg ccccctaccc tcgatggtat tatcgcgctc gtcttctgcg
     7081 agccggtatg aaacaagcct ccgagagcct ccacgcaacc gtccacggcc gcgtgcaggg
     7141 cgtgaacttt cgttactaca cgcagctcaa agcgcaagcg ctgaacctga ccggatgggt
     7201 caggaaccgg ccggacggca ccgtcgaagt cgtggccgaa ggtgcgcgtt gggcgctcga
     7261 ccagttgctc gactttctgt accacggccc cagccacgcg catgtcgatc gcgtcgacgt
     7321 cgagtggctg aaggcgaccg gtcaattcac ccacttccag gtcaccggct aaatgaacca
     7381 caaccccgag cgactggcct ggaccataat gctgctgtcc tttggcatgt gcgtggccct
     7441 cgccatcagt gtgccgttgg gaattcgatc gttcctgacg gacagcaccg accctgcccc
     7501 gctagtgctg gacgtgcagc aaggggccgt tttgctcaag cggccggcct tcggcgactt
     7561 tatcctggtc cccagccaga ctacggacgt gcctgatggc tccatcattc gcgccgatca
     7621 agccacccag gccattctga ccatgcgcga tcccgacctg gtgacgaatc gcgtggttgt
     7681 ccagctatac gccaacaccg aggtcacctt ggagcgggcg aattcgccac gcttcggcgt
     7741 cagtcccaac ccccatcggc tcgacctgtt cgtgaccagc gggcgggttc gcatcaacgt
     7801 gctaaacggc acgaatcggg gagtgacggc gacggcgcgt tcaccgcaag gtgaagtgac
     7861 cttcaccacg ggtacctacg cgttggaagt caccaacgag gaacttcaaa tcacggcgcg
     7921 cgaaggccag gcgacggtca gcgcgcagga ctcgtccgtc acgatcgaac cttcgcagcg
     7981 cgccgtcgtg cgcctgggac aaccgccggt aggcggcctg gccgacgaac ggccactgat
     8041 cgtcaacggc agttttgctg cgccccttga gtcggcgtgg cagatcgagc atgtcccgct
     8101 tggctcgccc gaacctaccg gcagcgtcat caccacgacc atcgtcggcc ggcacgcggc
     8161 cgtgtttgaa cgcaccggtc caagccacgc cgagacgcgc ctggcacagg accttaatcg
     8221 cgacgtgacc gacgccgctt cgctcaccct gcatttcgcc gtcctggtca attctcagga
     8281 tgtgccagtg tgcggctcgg tgggcaccga atgcccgatg atggtgaaaa tcgactatcg
     8341 agatacctcc ggcgcgaatc gcgagtggct gcgcgggttc tacgccgtaa atgacccgaa
     8401 caatatcaac aaacccttct gcggtagctg tacgcgaccc tttgagcacc agcgcgtcct
     8461 cagcaatatt tgggtctcct acgattcggg caatctgatc gacgtgctca ccacacccga
     8521 cggcttcaaa ccgacccgca tcacgcgcat caccttctac gcctccggcc actcgtaccg
     8581 atcggcgatc accgacgtag aactgctgat gcaggattga cgggcagggg cacgctgtgc
     8641 gcctgcacct acggcccatc gacgaagaat ggtgctgttc atcattcgaa tgccaggccc
     8701 cgctccttca agctcacgaa acgctgatgg ccgatgatca ggtgatcgag cacctcgatg
     8761 tccagcagct ttccggcatt gatgatctcg cgcgtcaagg tcacgtcctc gggcgagggt
     8821 gacgggtcgc cggatggatg attgtgcgcg acgatgaggg ccgcggcgtt ttgccggatt
     8881 gcgtcgcgga acacttcgga gacgcgcacc cacgtcgtat tcaggctgcc gacgtacacc
     8941 gtcggcgtcg ccagcacgcg gttgcgcgtg tcgagcagca tcacgcgcaa gtgctcctgc
     9001 tcaaggagcg ccatttcgga tagaaggata ttcgcggcat cggcaggcgt gcggatctgg
     9061 ggacgctctt cgggcgtgga cgcgagcagg cggcgaccca gctcgaaggc cgccttcatc
     9121 tgcacgacct tggcctggcc caatcccttc tcctgcgaaa gctcggcgac cggtctgcgg
     9181 gcgaggcccg ccagaccatg cttgttcagg aggcgctgcg ccaggttgat cgcgttctcg
     9241 ccgccggtgc ctacgcgcaa gatgatcgcc aacagctcgg cattggatag cgcgctcgcg
     9301 ccggcatgcg ccagccgttc gcgcgggcga tcgccgctag gcatgtcttt gatcgtcgaa
     9361 ccgtactcga cctcttccat catgccgtgc cctccttccg cgcggactat acttcacctc
     9421 gccccggcgt gtcaagcagg cgccttcctt tggtggtata atctcgcctg tttgcgctcc
     9481 agtctcaggg agagaccacc gacatggact ttcagagcat cgtcctggca ttcctggcgt
     9541 tcagcggcgc gttcctggca gcgctgtggc tggcgctgat catctggact tttcgagaca
     9601 tgcgctcgcg ctcgcgcgac atcttcgcgc agatcctcgc cgcgctgctg gtcgccgtgc
     9661 tcactctgcc ggggctgctg atttacctga ttctgcgccc gaaggagacg ctggccgaag
     9721 cctacgagcg ctcgctcgaa gaagaggcgc tcctgcaaag catcgaggac gtcgagcact
     9781 gcccggggtg cggcagtcgc gtccattccg attacctggt gtgcccttcc tgcaacacca
     9841 agctgaagaa ggcttgccgc tcgtgcggtc gggccatcaa tctgcgctgg aacgtgtgcc
     9901 cgtactgcgc cacgcctcag atcagcagcg tagcgcccgt ccaggcaaca acagccgaaa
     9961 cggcggagac gacacagcgc atggccgtgc gccgcgaaga gagcagcgaa gtggcataga
    10021 atcctcccgc gggctggagc ctgcgggagg ttttcatatc acgtgaacct tcagcatatt
    10081 cgtccggtat ctctcgccgg ccggaatgcc cgccgtaatg acgatggtgt ctcccctccc
    10141 ggccaatcct gactcctcgg cgagttgcgc ggcgtacgcg agcatgtcat ccgtggtctc
    10201 gtaatacggc accagcacgg gcctcactcc ccaggccagc gccagttggc gctgcacctc
    10261 ctggcgaggc gtgattgcca ggaccggcgt cctcgggcgg tagctcgcca cccaccgcgc
    10321 cgtgctgccc gtataggtgg acgtgacgat ggctttcgcg cccagctcgt aggcaatctc
    10381 gacggtggcc cggctaatcg ccgcagccgg cgatgggtca cgctccagcc gggcgcgccg
    10441 gatcgtctct tcgtagggca ggtgcgcttc ggccgcttcg gcaatgcgcg ccatcgtctg
    10501 aaccgtctcc aggggatact cgccggtcgc ggtttcggcc gacagcatga ccgcacttgt
    10561 gccgtcgaga atcgcgtttg ccacgtcgct ggcttcggcg cgcgtcgggc gcgggcggtc
    10621 gaccatcgaa ttgagcattt gagtggccgt gatgaccgga acccctgcca ggttgcagcg
    10681 cgcaatcaga tccttctgaa cgaacggcac gcgctcggcc ggaatctcga tgcccaggtc
    10741 gccccgggcg atcatgaccg cgtcgctgcc cgcgaggatg tcatcgaatg cagcgatcgc
    10801 ctccggcttc tcgatcttgg cgacgatcgg tattcgcgca tccagattcg ccagccaatc
    10861 acgcaactgg cgcacatcgc gatcgctgcg cacaaaggac aacgccagga aatccaggcc
    10921 ctgctccacg gcaaagcgtg tatcatcacg atctttgtcc gtcaaggccg atagcgacag
    10981 gtcggcgccg ggcgcggcga ctcccttata cgagccgagc ggcccgccgg tgacaacgcg
    11041 gcagcgcatg tcagtgcccg tggtttcgag cacgacgaac tccaactgcc cgtcgtcgag
    11101 cagcaggcgc tggccggggc gagcttcgcg gatcagctcg gcatgcggca agtgcacctc
    11161 accccgatcg cccggcacgt cgcgcgtcgt caaggcgatt tcgtcgccga tacgggtcat
    11221 gacgtaatca ccacgaatcg cgccgacacg tagcttcggc ccctgcagat cccccaggat
    11281 ggcgacgact gcgttttctg ccagggcagc ctgccggatc gcggcaatgc gcgccatgtg
    11341 cgcggtgcga tcgccgtgcg agaagttgat gcgcgccacg ctcatgccgg cgcggatcat
    11401 cacccgcagc atctcgacct ggctggacgt cgggccgatg gtgcacacaa tcttcgttct
    11461 agccaacttg tccactcaca agcagggtgg cctccttcgc tccccggacc atgtcgaagt
    11521 gagggccgca ccttgatgcc ggttgcatac tcgtggtacg agccggtcaa tcctcgacga
    11581 cgtaggccgc gccgagcgcg cccggtccgg catgcgccgc gaccgcaggc ggcatctcga
    11641 tcaccataca ctcgccgaga ccatatttgc cgcggaattc atgagccagt gcctcagcct
    11701 ccccgtgcag ccccgtgtgc gtcacggcca gttggaactt gctcttcccg tgcaaggctt
    11761 cgtcgacaat gccgcgcagc cgcgcaaggg ccttgtttct tgtgcggatg cgttcgagca
    11821 cttcgatgtg gccggtgcca tttcggatcg agaagacagg cttcaactgc aacatggtac
    11881 caataatgcc ttgaatccgc gtggcccgcc cgcccttcac cagatactcc actgtgtcga
    11941 ggacgaaatc gagcgtcgta ctcgggatca gcgcgtcgag gcgcgacgcg atctcgggcg
    12001 ccgactttcc atcccgcgtc atgtgcgcgg cttccagaac gagcagcgcc tcggccgccg
    12061 cgacggtctt cgaatccacc agcgtgatcc tgtcgctgcc aagctccttc ttagcggtct
    12121 ccgccgagtt gtacgttccg ctcaagtagc tggacactgt gatgcacaga atctcgtgcc
    12181 ccgcgtccag gagcggcttg aagatgcgga ggaagtcgcc cacggatggc tgcgacgtgg
    12241 tcggaaacgc tttcgacgcc ttgagtcgct ggaggaagac atcgttcgtg atgtcgaccc
    12301 cgtctcgcag cgtctccgag ccgaacatga cgatctgagg gaccaagtct acgccgtgct
    12361 gcgcggcgac tcctttcgtc aggcccgtcg tcgtatccga gatgattttt agcataattc
    12421 ccccccatgt gcgatcggtt tactagcggt acagcataca acaccggacg ccgcaaaaag
    12481 ccacgcccgg cccgatcagc acgttttcct ggcactggct cacgtctcgc ccccggcgcc
    12541 gggtgggcgg cggcctgaga gcgcgtctag aaccgttcgt accgcatcaa gatgggggtc
    12601 gatgacaagc gcggagggcg ctgccttgac ggccgccggc acatctttga ggccactacc
    12661 cgtcagaagg acgaccaccc gctcatccgg cccaatctga ccggcctgga ccgccgatgc
    12721 cagcccggcg tacgccgctg ccgccgccgg ttcggcgaac acggcggcct cctgcgccag
    12781 ctcgcgcatg gcggccagga tgtcctcgtc gctcaccgtg acgaacgccc cacccgtctg
    12841 ccgaacggcc ctgatcgcgc gccggccatc tcgcggcaga tcgacgctga tgctgtcggc
    12901 aatcgtgtgc gccgatacgg ccgatacgtc gtccgcgccg gccgaccagg ccctgtagca
    12961 cgccgccgcg ccctcagcct gcacgcccat cagcttcggc atccggtcga tccaacgcag
    13021 ctcggcgaga tcgaccagcc ctctgtggag gcccgaaatg atattgccgt cccccaccga
    13081 cacgaaaatc cggtcgggcg catcccaaat ttggaagttg gaggctggag gttggagttt
    13141 ggaaagcgtg agctgctcgc atatctcgag cgccgccgtc ttcttgccct cgaccgtgaa
    13201 ggggttgtag gcggtgttgc ggcaatacca gccgaacgcc ttcgaggcct ccagacacag
    13261 ctcgaacgcc acatcgtagt tgccccgcac caggaacacc gtcgcgccgt agatcagcag
    13321 ttgcgcgact ttggcctgcg gcgcggattg cggcacgaag atcaccgtcg gcatcttcac
    13381 gctggcggcc atgcccgcca gggccgcgcc ggcgttgccg ctcgacgccg tcgtcacgat
    13441 gtgtcgccgc tcctgcgcgg cccgcaccac gacgagcgcg ctggcgcgat ccttgaacga
    13501 agcagtcggg ttgcggccgt cgtctttgag ccagacttcg tgcaggccga gtttcgcctc
    13561 gaggcgcggc gcgcggtaca gcggcgtcca tccgacctga agcggcaggc cttcggattc
    13621 caggtctatc ggcagcagcg gattgtagcg aaacatgttg agactggccg aagtctgcat
    13681 gtttgcaagt tggccgggcg aggcttgccg ggcgatcgcc ctgtaatcgt aaacagtgtc
    13741 gagcacgcct gcgtcgccgt gcttcgggca gacgtactcg acctcacccg gatcatactc
    13801 tgtgccgcag atcgtgcacc tgaatccctt gacgttgttc atttcggctc cattcgaata
    13861 tcggccacca atccgaagtg gtcggacgga tacagccgct cgtcgtccgg cgaaggccgg
    13921 ttgaaggcca cttcgcaccg ctccactctc acggcgccat cgacaaagat gtagtcgagc
    13981 gtgcctcgcc acgagtcgtt cgtgcccttc aacagcaatc ccgatagccg ttcgagcatg
    14041 cgccagagcg ccgcatccat ccttcgtcca cgagcgagcg gcgtcgggca ggtgtactcc
    14101 ggttcctgcc catgcgcaag cgcgtgagcc gatgcaaagc gcgccttcat cgcagctatg
    14161 ctcttgaagt gcggttcggc gttgaagtca ccgcacacga ccgccggtgt gtgattcgga
    14221 atccaatcga ggatgcggcg tgcctgctcg agacgcggcg catcatcaag cggattccag
    14281 tacagatgcg tattcaccaa tacgaagggc gcgccgttgt gccggactgc cacccgctgc
    14341 gccacgcgat cttgtgcaac gagcgggagc gcccggtgtt cttcgaccgg caagcggctg
    14401 aggatgccaa gcgcttcgcg ctgccctttc gcgccggtct tggggcacag atgaacggaa
    14461 tagccaccga gctgatcggc aatccactgc gcgttgtccg ccggcagcgc gacctcttgc
    14521 agcgcgatca ggtcaggctg gatcgctcga aactcctcta cgatcaacgg cgcgcgctca
    14581 cgccaccggc acaggtcgac gaggatattc agcgtcgcca ctcgaagtgc gccattgtgc
    14641 ctcgtgcgcc tttcatcctt catcccttaa ccttcaggtc cgccgctccg aacgcgcgcg
    14701 gcaatagctc gtgcagcgtc gtctcatgga cgaccatgcc tgcgccatcc acgaggaaca
    14761 ccttcagatc gagtccgaac tccgacagca tctggcggca cgcgccgcac ggcgagccgc
    14821 cgttgtgcgt ggcgacggcg attgcttcga atcgccgctc gccttccgac accgccttga
    14881 ccaacgcggt gcgctcggcg cagataccta ccggatagac cgcgttctcc acgtttgcgc
    14941 cgtcgtagat cttgcccgag acggccaaca gcgccgcgcc gacgcggtaa ttggagtaag
    15001 gcgcatacgc ctgcgcccgc gcagccatcg ctcgcgcgat cagcactgac tgaatatcat
    15061 caggcatctg tcgatctcct tctcctcctc ggatcacttt gagcgaggtg acatcgcagc
    15121 cccacacgcg ggagagtcaa gcatccaact tccggcttcc aacctctaac ctccaacctc
    15181 taacctccaa ccctatcact cacccacgat ctgtgccacg acttcgcgcc ggcggggccg
    15241 cgtatcgaaa tcgacgagaa cgatctgctg ccacgtcccc agtgccaagc ggccgtcggc
    15301 gaacggcacg ctgagcgagg ggcctagaag ggctgcgcgc acgtgcgaat ggccgttgtc
    15361 gtcgccccag cgaagatgat gctggtatac gccgtcgcgc ggcgcgactc tctcaaaggc
    15421 cgtttgcagg tcggcgacgg cacccggctc gaactcaatg gtcgtcagcc cggccgtgct
    15481 gccgacgacg aacagcgtca cgacgccgct gcgcgcgccc gactcctcca ccgcccgcgc
    15541 gacggcatgg gtcaggtcgt gcgtgtcgcc gtcgcctctc gtttgcagcg ttaactgttt
    15601 agtgataacc acgcttcacc tggctcggat ggatccacag tgcccctgat tataccgcgt
    15661 gatgggcacg cagcgtgcag ccacgcccgg cacgcggtat aatcgcatca ctatggttat
    15721 cggcgattac cacatccgtg tcgaaaaaga ctacaccgtc ttctcagccg cgcacttcat
    15781 cacctacgac ggccaccagt gtgagcagtt gcacggccac aactaccgcg ccgccgccgc
    15841 cctggcgggc gacctggacg aaaacgccta cgtgttcaat ttcgtgccac tcaagcgcgc
    15901 gctgaagggc gtcgtcgatc agcttgacca caagaccctg ctgccgcaag acaatcccag
    15961 gctgcaggtt cggatcggtg atgagtcggt ggaagtcgcc tatccaagca agcacaagcg
    16021 ctacgtgttt ccgcgcggcg acgttgccgt gctgcctatc ccgaacacca ccgccgagat
    16081 gctggctgcg tggatctgcg ggcagttgcg ccaggcgttg agagcctacg atacgcgcgg
    16141 catccgatcc atagaagtgg aagtcgaaga atcgcccggc cagcgggcga tttaccgcga
    16201 ggagtggaac tgagatgagc gagccgcaca ccgtctattt ctcgctcgga tccaacttgg
    16261 gcgaccggca gagcaacctg atcgaggcga tccagagcct gcgcgccaac gtgcagatcg
    16321 agcgggtatc gtccgtctac gagagtcgcc cggctcacgc cgccgatcag cccatgtttc
    16381 tccacgtcgc cgtgaagggc acgaccgatc tcgaacccgc cggcgtgatt cgcttcatcc
    16441 agcgcatcga gcgccgcctg gggcgcgtcc gccccgaccg gcatgtgccg tgcccgatcg
    16501 acatcgacct gctcagctac gatgatcacg tcgtgtcgag cgatgggctg acgctgccgc
    16561 atccatccct ggccggtcac gcccacgtgc tggcgccgct tgacgagatc gcgcccgagt
    16621 ggaagcaccc gacgctgaaa cgcagcgcgc gccagttgct cgacgactcg ggcggatcgg
    16681 atggtgtgaa gcgcctggcg cacggcctca ccgcgcggcc cgaccgcgac gtgcagtccg
    16741 aggagccgcc ggcgctgctg cgactggatc gggtcggcgt gaccggcttg cagcgcgtcc
    16801 ttcggttatc gaccgtcaaa ggcagcctgt accacgcgcg catcgacctg tccgtcgagt
    16861 tggggccgga acagaagggc gcccacatgt cccgcttcag cgacacgctc gaagaggtca
    16921 tcgacgagat cggcggcaca gaagccgcga cggtcgaatc gctggccgag cgaatcgccc
    16981 gccaggtcgt gagtgaccag cgcgctcgac gcgccgacgc cgaaatccac gcccactttc
    17041 cgatgtacaa gcacgcgccg ctgtcgggca agcggacgca ggagatctac acgctcatcg
    17101 gcatcgccgc cgccacgccc gatcggagcg ttggcgtcat cggcgtggaa gccgaaggga
    17161 tgatggcgtg tccgtgcgcg caggacatgg tgcgcgcggc ggcgcgtgag cgcttgctcg
    17221 aagacggcat cgacgaggcg atggccgacc gcgtgctcga tctcgtgccg ctggccaccc
    17281 ataaccagcg cgggcgtggc cggctgctgg tcggcctgcc ctacccgatg cgccccgccg
    17341 atccgccggc cgtccgcgcc caggacctcg tcgagatcgt cgaggcgtcg atgagcgcgg
    17401 agagctacga tctgctcaag cgcccggacg agctgttcgt cgtgcaaaaa gcccatcgcc
    17461 acccgcgctt cgtcgaagac gccgtgcgtg agatgctgaa taacttcgtc gagatgtatc
    17521 ccgatctgcc ggatggcgcc tacctctcgg cgcggcaggt caacctcgag acgattcaca
    17581 agcacgacgt gttcgccgag cgcggcggca cgctcgccga tctgcgcagc gagattcgcg
    17641 gcggcagcgg gcggactttg acgctagacg cgtggctggc cggacagctc ggcgaatcag
    17701 acttgatcga tccggcgtaa gggctggcat ggaattctta ccacccgtcc agactccgtc
    17761 agcagcccga gcagttaggc tggaggcacg aatgttgcct tttccgccgt tactcccgta
    17821 tcgtgtgttc ccacgtttcc tttcgggcct tgcttagaaa cgacgcgaag tgagtgccgc
    17881 cggtgccctt tgcctcgtcg gggttcggcg cctggtgaga gatgtaccgc acggccagct
    17941 ccatgtgctt cttcctgaac cgatccagct cgcggatgca cgcattatat cgatcaccca
    18001 gcgcgggaca ctgcgccgcc cgatccagaa cgaattgccg gacagaagga ccattttcca
    18061 gggcctcgat gaagcggcgg tgccccgtga ccatatagtt gcgcatctcc atcagaaacg
    18121 ggctggtctc gatgcttctg tgcgtgatgc ccaggccggc gtccagcgcc tgaatgaggg
    18181 ggctttgtgc ggcgctgccg cccgtgcaca tgcgcggcgc ttcgctcacg ccttcgtaga
    18241 tcacgccagg ctccagccag ccggtctgat agggtctgac ccggtggtag aagatgtacg
    18301 gatcgcactt ctccggtatg cgctcgagca catcatagac tctggccagg gccgacgcga
    18361 tcgtttcgag gcgcgccgcc aggtcgtccg cccggcctcc ggcaacagcc tgctgcgccg
    18421 cgacgagcgc tcccagcgcg ggcgcaccct tcgcttcgat gacgaccgcc gtcaaataga
    18481 accactgctc gtccatgccg cccaggaaca attgcagcgt ggccaggttg tccagctcga
    18541 tcgggccggt tttgtccagc cgccgccagt taaacagcac ggtcgaagga tgggcggcta
    18601 tgggcgggcg tcccagcttc tcggcgaggc ggcaccacgg catggccacc gcgcgcggca
    18661 ccttgcgcgc cggctcgggt cccgcccaca cgtaggcgtt gccgaacacc gacaggagca
    18721 gcatcgcacg ctccaactgc atctcatctt caagtcgctc gatattcagc atcgggagcg
    18781 cctcgagcgt gggccgcagc cggccggtca tcagcagtac gggcatgtcc ggcgcggcgc
    18841 gctcccacgc gtcgaagcca gcgggcagtc gttcgagcgg atctttggcg ggcaggaaac
    18901 cgcgcacagg gtcgatgtga tatgccgcaa ggtctaccgt agccactaca cctcccgaac
    18961 gtaccgcagt cgccaagggg gagctattcc gatccgaaga actcgatcag cgagcgcgac
    19021 agcctgtcgt gcgaaatcct ctccagcggg tgaggcgttc cgggcaagac ttcaagctcg
    19081 ccgtgaggaa gagcgcggta gacttccacg cactctgcaa cgcccacggt gctgtcccgg
    19141 tcgcctacca tgatgcgcac gcgctgctcg atgcgcgcta aatcgtcccg cgcgaacccg
    19201 ccgcgctcgg cgagtgacca gagcgagccg gaagtttctt tcagcacggt ctcccatccg
    19261 gcagccgtat gtcgctcggc cagcgcgcgg gcgtagtgtg gaaccttcgc agcgatctta
    19321 tgcggatcga gcagcgccgt ctcgcgcgcg gcgatgccgg catcccagaa gaacttcgtg
    19381 ccaagcgtag ccacgcgctc gacccgctgc ggatgaccgg cggccagcga gcacgccacg
    19441 taaccgccca tgctgtaacc gaagatgctc gtgctcccaa taccgtgggt gtccaggtac
    19501 tcgacgacgt tctggatgaa gtgctcgatg cgaaacgggc gcccgcggcc cggcgcgcgc
    19561 ccgtgccctt cgaagtccac gaggtgcagc ttgtagtgat cggcaagcgg cagccacagc
    19621 ggggcgaact gatcgccggc gccgagcgcg ccgtgcagca gcagcaggtt cgatccagcc
    19681 aaatgtgcgc tcctaatacg tgaccacgcg ccgcagcttc ttggcctgtg agacccaatt
    19741 cttgacctga gcctgcgccg gcagcttgcg aactcgcttg tggtcctggt tggcggcgac
    19801 gagcttctcg tataggtggc ctggcacgcg ctgcgccagc tcgacgaccg tgtcgactcc
    19861 ggcggcctcc agcaaatcgg cttactcctc tccgaccccc ttgatgcgga acaggtcgac
    19921 gtggttcatc cagcgcagga tcagcgcctc gccaatgcca cttctggcgg cgatctcctg
    19981 gcgacctttg ggcgtggcgc cggccgcgag cagggtctgt gcggagcgga cgccagccgc
    20041 tttcagtttc ccggcgtaga cctcgccgat gccttcaacg gatgcgatga cattgctctg
    20101 ggtcaccaga tagtctacga gcgtatcgac cgtgaatctg acgaggttct gatagtcttc
    20161 ctggccgacg aagtgaaagc ccactttctc ggagtcgtcc gcgccaaagc caacgcgtgc
    20221 gcgcggcggg cttggatact tcgccaggaa gacgtcgcgc atccacgact ggcaatcggg
    20281 gttttcttgc atctgggcgg agaaataatt gtcgtagacg ataagccagg ctgccgggcg
    20341 taagactcta ctcgcttccg caaagaacgc ctcccggtcg atttaacagg ttgaatggaa
    20401 cacgccgccg accttcttgc ctcgtttcat caagtcgttg aacaactcta ccgctcgccc
    20461 ggtttcttcg atgtgatact cgattcctcg ggagc
//
LOCUS       DBNHXB010000160         3532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3391844, whole genome shotgun sequence.
ACCESSION   DBNHXB010000160 DBNHXB010000000
VERSION     DBNHXB010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3391844"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="AC162G_06275"
     CDS             <1..1263
                     /locus_tag="AC162G_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5075451.1"
                     /translation="DWQIEAAVRRPGAFDAFAAYRVKVGLDGRIAPAGEPTLVESIVR
                     WLSIYGLAFGGFVAIALGFVWLAIGAKASRNLTSLALLAIPAIIALPIGALSVYTFFK
                     EATPGLTLTNPYLPDEQSLAIGQQLFEANCAACHGSAGRGNGPRAGELTVRPADFGSG
                     HLDIHTDGDVFYWIQNGLPTVNSPMPAFKNILTEDETWHLVNYVRRLRNLAGAASSSA
                     SEAAAAPPSALQPYTPPSFIAPPEAAAGVTPQRPTPAASGDPAALERLARADAAMNVL
                     RSLVEKQSLRDDAGNRLDVVFTYAAPDRMRYQIVNGATAIQIGSDDYQLMPDGAWIRN
                     QRAIRFEWPKFNYSSLAGEARIEGEEQIGESTATIVAFQYGGFDFRMWIDQRTDRIVK
                     LAMDGPNHHMESAYSDFDSAPPIERPTP"
     gene            1280..1513
                     /locus_tag="AC162G_06280"
     CDS             1280..1513
                     /locus_tag="AC162G_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075452.1"
                     /translation="MEYASVADLLANVLFLPSLHGELGVLDELLLFCLPLVVVLVVLA
                     ATAQRARRKEEKMRSQTKAESYGDTQNMEKTDP"
     gene            1571..3025
                     /locus_tag="AC162G_06285"
     CDS             1571..3025
                     /locus_tag="AC162G_06285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR5075453.1"
                     /translation="MKDLMMRAIDRASQRGAQYADVRIVRTITESLSVKNGIVDALNV
                     DESIGFGVRVLAGGNRGAWGFASSRDLTADEVDRVTDRAFEIAKASALVAGDSPVDLG
                     PPVASRGTYVTPLQIDPFSISIEDKLGVLMAADAEMAKVKGVKTRQGSLVFIQENRLF
                     ANSEGALTDQTIAETGGGIVATAVGGDEVQRRSYPTSNGRQQVTAGWEVVLGWNLPGN
                     AGRVASEAVALLSADPCPRDIRTTIILGGDQVALQVHESCGHPIELDRVFGTEAAFAG
                     TSFLTTEKLRAFRYGSEHVNVTAEAVRPYGLGTFGWDDEGVPAQSTPIVKDGWFVGYL
                     MSRETAPRLGLTSNGCMRASGWNRIPLIRMTNVSLEPGTWTLDDLVADTGDGIYMETN
                     RSWSIDDKRYNFQFGTEIGYEIKNGKRGRLLRNCTYTGITPEFWNSCDAVCDADHWVM
                     WGTPNCGKGQPLQVAHTGHGAAPARFRNVRVGVV"
     gene            3085..>3532
                     /locus_tag="AC162G_06290"
     CDS             3085..>3532
                     /locus_tag="AC162G_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PmbA/TldA family metallopeptidase"
                     /protein_id="HIR5075454.1"
                     /translation="MLLGHNHAEDLLKRALRFSQAGETQVLVSAHESYLTRFANNVIH
                     QNVAEANVGVTIRAVVGRRSGTATTNRTDDEALAQAAARALSHARRQPEDPDFPGLPD
                     PQPIEPAPSFDEEVASLAPEGRARSVGVVCDRAAEHKFAASGAFRTS"
BASE COUNT          646 a         1127 c         1178 g          581 t
ORIGIN      
        1 gactggcaga tcgaggcggc cgtgcgccgc ccaggcgcgt tcgacgcgtt cgccgcctac
       61 cgcgtgaaag tggggctgga tgggcgcatc gcgccggccg gcgagccgac gctcgtcgaa
      121 tcgatcgtgc gctggctttc gatctacggg cttgccttcg gcggcttcgt cgcgatcgcg
      181 ctcggtttcg tctggctggc gatcggcgcc aaggcgtcgc gcaatcttac ctcgctggcg
      241 ctactcgcca taccggcgat catcgcacta ccgataggcg cgctatcggt ctacacgttc
      301 ttcaaagaag cgacgcccgg gctgacgctc accaacccgt atctaccgga cgagcagtcg
      361 ctggcgatcg ggcagcagct cttcgaggcg aactgcgccg cgtgccacgg cagcgccgga
      421 cgcggcaatg gcccgcgagc gggcgagttg accgtgcgtc ctgccgactt cggcagtggc
      481 cacctcgaca ttcacacgga cggcgacgtg ttctattgga tccagaacgg cttgccgacg
      541 gtcaattcgc cgatgcccgc cttcaagaac atcctgaccg aggacgagac ctggcatctg
      601 gtcaactacg tgcggcggct gcgcaacctg gcgggcgctg cgtcgtcgag tgcgtccgag
      661 gcggctgcag cgccgccgag cgccctccag ccctacacgc ctccatcgtt catcgcgccg
      721 cctgaggccg cggccggcgt cacgccgcag cgccccaccc cggccgcgtc cggcgatccc
      781 gctgcgctcg agcgactggc ccgcgcggac gccgcgatga atgtgttaag atcgctggtg
      841 gagaaacagt cgctgcggga cgatgcgggg aaccggctgg acgtcgtctt cacgtatgcc
      901 gcgcccgacc gcatgcgcta ccagatcgtc aacggcgcga cggccatcca gatcggctcg
      961 gacgattatc agctcatgcc ggatggcgcc tggatcagga accagcgcgc gatccggttc
     1021 gagtggccga agtttaacta cagctcgctc gcgggagagg cgcgcatcga gggcgaggaa
     1081 cagatcgggg aatcaaccgc aacgattgtc gcattccagt acggaggctt cgactttcgc
     1141 atgtggatcg accagaggac agaccgtatc gtcaagctgg caatggacgg cccgaaccac
     1201 catatggagt ccgcgtacag cgacttcgac agcgcgccgc cgatcgagcg gccgacgccg
     1261 taacaacgtg gtgaagtcca tggaatacgc cagcgttgcc gatctactag ccaacgtcct
     1321 gttcttacct tccctccacg gcgagcttgg tgtgctcgac gagctgctgc tattttgcct
     1381 gccgctcgtc gtcgtgctcg tcgttctggc ggcgacggcg cagcgcgcgc gccggaaaga
     1441 ggagaagatg cgaagccaga caaaagcaga aagctacgga gacacgcaga acatggagaa
     1501 aacagatcct tagttccttt agttccctca gttccctgag ttccctctgg gctcaatatc
     1561 ggaggttgcg atgaaagatc tgatgatgcg tgcgatcgac cgggccagcc agcgcggagc
     1621 acagtacgcc gacgtgcgga tcgtgcgcac gattaccgag agtctgtcgg tcaagaacgg
     1681 catcgtcgac gcgctgaatg tggacgagag catcggcttt ggcgtgcgcg tgctggccgg
     1741 cggaaaccga ggcgcatggg gcttcgcctc cagccgcgac ctgacggcag acgaagtcga
     1801 tcgcgtcacc gatcgcgcgt tcgagattgc caaagcctcg gcgctcgtcg ccggcgacag
     1861 cccagtcgat ctgggcccgc ccgtcgccag ccgcggcacg tacgtcacac cattacagat
     1921 cgatccgttc agcatatcca tcgaggataa gctgggcgtg ctgatggccg ccgacgccga
     1981 gatggcgaag gtgaaaggcg ttaagacgag gcagggcagc ctggtcttca tccaagagaa
     2041 cagactgttc gccaacagcg agggcgcact caccgaccag acgatcgccg aaaccggcgg
     2101 aggcatcgtt gcgacggccg tcggcggcga cgaagtgcag cgccgctcct acccgacctc
     2161 gaacggtcgc cagcaggtga cggccggctg ggaggtcgtg ctcggctgga atttgcccgg
     2221 caatgcgggg cgcgtcgcga gcgaagctgt ggcgctgctc agcgccgatc catgtccgcg
     2281 cgacatccga acgacgatca tcctcggcgg cgatcaggtc gcgctgcagg tacacgagtc
     2341 gtgcggccat ccgatcgagc tggatcgtgt cttcggcacg gaagcggcgt tcgccggcac
     2401 gtcgttcctc acgacagaga agctgcgcgc gtttcgctac ggctccgagc acgtcaacgt
     2461 gacggccgag gccgtgcggc cgtacggcct gggcaccttc ggctgggacg acgaaggcgt
     2521 cccggcgcaa tcgacgccga tcgtcaagga cggatggttc gtcggctatc tgatgtcgcg
     2581 cgaaacggcg ccgcgcctgg ggctgacctc caacggctgc atgcgcgcgt cgggctggaa
     2641 tcgcatcccg ctcatccgca tgacgaacgt cagcctggag ccggggacgt ggacgctaga
     2701 cgatctcgtc gccgacaccg gcgacggcat ttacatggag acgaaccgct cgtggagcat
     2761 cgacgacaag cgctacaact tccagttcgg gacggagatc ggctacgaga tcaagaacgg
     2821 caagcgcggg cggctgctgc gcaactgcac gtacaccggt atcacgcccg agttctggaa
     2881 ttcgtgcgac gcggtgtgcg acgccgatca ctgggtgatg tggggcacgc ccaactgtgg
     2941 caagggccag ccgctgcagg tggcgcatac cgggcacggc gcggcgccgg cgcgcttccg
     3001 aaacgttcga gtcggtgtgg tgtagtctgg aaggtggtga ttggtgattg gtaattgggg
     3061 gcccgcgcca ttggagaggc aattatgcta ttaggccaca accatgctga agacctgctg
     3121 aagcgcgcgc tgcgattctc gcaagccggc gagacgcaag tgctggtcag cgcgcatgaa
     3181 tcctacctca ctcgattcgc gaataacgtg attcatcaaa atgtcgccga ggcgaacgtc
     3241 ggtgtcacga ttcgcgccgt ggtgggcagg cggtcaggca cggcgacgac gaaccgcacg
     3301 gacgacgagg cgctggcgca ggctgccgca cgagccttat cgcacgcacg ccgccagccg
     3361 gaagaccccg acttccccgg cttgcccgat ccgcagccga tcgaaccggc gccttccttc
     3421 gacgaagagg tggcttcgct ggcgcctgaa gggcgggcgc gaagcgtcgg ggttgtgtgc
     3481 gatcgggcgg ccgagcacaa gttcgccgcc tccggcgcgt ttcgcacctc gg
//
LOCUS       DBNHXB010000161         4082 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3056157, whole genome shotgun sequence.
ACCESSION   DBNHXB010000161 DBNHXB010000000
VERSION     DBNHXB010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3056157"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1057)
                     /locus_tag="AC162G_06295"
     CDS             complement(146..1057)
                     /locus_tag="AC162G_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR5075455.1"
                     /translation="MSDSKLMTLAEAVSRFVPDGGAVVAGTALESFIPFAGGHEIIRQ
                     RKRDLTLIGPISDILFDQLIGAGCVRKVRAAWVGNVITGSGYNFRRAVESGAIEVEDH
                     SNLTLALALRAAAMGVPFMPARTALGSDLFKTNASLKTTTCPFTGDTLTAVAAIAPDV
                     AIVHVQRADEHGNAHAWGNLGVTREACLASRHVVITAEEIVSHEVITSDPNRVVTPGF
                     RVSAVAHAPWGAHPSPVPGHYNRDHQMFLDYQEESQTPEAFAQWQARWVDGVRGYEDY
                     LARLGEERVVGLRPKHHAPAEIVDYGY"
     gene            complement(1188..2324)
                     /locus_tag="AC162G_06300"
     CDS             complement(1188..2324)
                     /locus_tag="AC162G_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR5075456.1"
                     /translation="MKIGIVGGGPAGLYFALLMKKRFPAHKVQVVEQDPADATYGWGV
                     VFSDRALAYLRDGDADFYADVVAGLEIWDDLAIVHKGRRTAIEGNTYSGIARLELLRI
                     LQEHCRRLGVDMQFERRVTDAASLADCDLLVGADGVNSVVRQAHRERFQPSLDVRPNR
                     YVWYGTHQVFPALTLIFREHAHGIFIAHAYRYNQTTSTFIVECDAATWSRAGFESMSD
                     AESRTTCQEVFEEDLGGQPLLSNRSTWRKFVVVDNRHWSHENVVLIGDALHTVHFSIG
                     SGTRMALQDALALYRAFEMYGEDVRVALQSFEQARRPVVDRFLAAARHSFIWYENIRD
                     RMHLDPLSFAYDYMLRTGKVDHEGLRKKDPAFVAAYEAQRTPHA"
     gene            complement(2321..2806)
                     /locus_tag="AC162G_06305"
     CDS             complement(2321..2806)
                     /locus_tag="AC162G_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5075457.1"
                     /translation="MAERTLAEMLDDLRLTQQVFLDILSRADEHILYRRPADGGWTLA
                     ETLAHIAEARQFFSEATRRVRAAPGTKMGRTVEHPGRLQNIEDHGHDAPNALRSRLIA
                     SHESLLKTLGEMTNADLRIQGEHVKYGPQMLSEFIERFVVEHDQVHVEQATALLTGDA
                     P"
     gene            3085..3726
                     /locus_tag="AC162G_06310"
     CDS             3085..3726
                     /locus_tag="AC162G_06310"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5075458.1"
                     /translation="MYAKSGTTIAAILGAAEKLFLARNYANVAMDDIAEAAGVTKGAL
                     YHHFRSKKDLYLAMMHSDLKQKQARMRAGVEMKGSCYERLRRLTESFLELPPEKRDLI
                     KLVRRDINIFKGPARDRLVRAYQAALPDQVEVIVRDGIRKGELADADPRVLAWMFVAI
                     VEVALTPYAGHVLRDPSTMLDFVMDMFFAGAGRPARHAISRMERRAARVSVAA"
     gene            3779..>4082
                     /locus_tag="AC162G_06315"
     CDS             3779..>4082
                     /locus_tag="AC162G_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5075459.1"
                     /translation="MAKMFINGQWADALRGQTVEVKNPANGTLVDTAPLAGKEDVEAA
                     IKAAQTAFPGWADTSPDDRAAILRKGIQAVEGHAQEIAALLTAEQGKPLFEASGELH"
BASE COUNT          780 a         1300 c         1253 g          749 t
ORIGIN      
        1 ttgaaagccg agcagcggca ggcgcatgcc gacgaagacc acatcaccat cattgatctc
       61 acgcgcggcc gccgctacca tcaattccgc cgccgtgtat gggcgctcgt aggggcgagc
      121 gcgaggctcg cccccatgcc cgcctttagt atccatagtc aacgatctcc gccggcgcat
      181 gatgtttagg tctcagcccg acaacccgtt cttcccccag ccgcgccaga taatcctcgt
      241 agccccgcac gccatccacc cagcgcgcct gccattgcgc gaacgcttcc ggcgtctggc
      301 tctcctcctg gtaatcgagg aacatctgat gatcgcggtt gtagtgaccc ggcacgggcg
      361 agggatgcgc gccccacggc gcgtgggcaa cggcgctgac gcgaaagccc ggcgtgacca
      421 cgcggttagg atcgctcgtg atgacctcgt gcgagacgat ctcctcggcc gtgatgacga
      481 cgtgccggct ggccaggcac gcttcccggg tcacgccgag attgccccag gcgtgcgcgt
      541 tgccgtgctc gtcggcacgc tggacatgaa cgatggcgac atcgggcgcg atggccgcga
      601 cagccgtgag cgtatcgccg gtgaaggggc aggtcgtcgt tttcaggctc gcgttcgttt
      661 tgaacagatc gctgcccagc gcggtgcggg cgggcatgaa agggacaccc atggcggcgg
      721 cgcgcagcgc cagcgccagc gtcagattgg aatgatcttc gacctcgatc gcgccgcttt
      781 ccacagcgcg gcgaaagtta tatcccgagc ccgtgataac gttgccaacc cacgccgcgc
      841 gcacctttcg cacgcagccg gctccaatga gttgatcgaa gaggatgtcg gagatgggac
      901 cgatcagtgt gaggtcgcgt tttctctggc ggatgatctc gtgtccgccg gcaaagggga
      961 tgaaggactc cagcgccgtg cccgcgacga cggcgccgcc atccggcacg aagcgcgaga
     1021 cggcttcggc gagcgtcatt aatttgctgt cagacaccgg aattcttctc cagcatgtga
     1081 gcgattgtcc ctcggccggc tgattccatc gaaccgtatg gagtgttctt cccaacgcga
     1141 gaggcagagc cctctgccgt cgagtctgga cgaagtgaat ggtctcgcta cgcgtgaggc
     1201 gtccgttggg cttcgtaagc ggcgacgaaa gccgggtctt tctttctcaa tccctcgtga
     1261 tcgactttgc cggtcctgag catgtaatca tacgcgaaag acagggggtc gagatgcatc
     1321 ctgtcccgga tgttttcata ccagatgaag ctgtgccggg cggcagccag aaaccgatcg
     1381 acgaccggcc gtctcgcctg ctcgaacgac tgcaacgcca ctcgaacgtc ttcgccgtac
     1441 atctcgaagg cccgatatag cgccagcgca tcttgaagcg ccattcgggt gccggagcca
     1501 atggagaagt gcaccgtgtg cagcgcgtcg ccgatcagca cgacattctc atggctccag
     1561 tgccggttgt ccaccacaac aaatttcctc caggtcgatc gattcgagag cagcggctgc
     1621 ccgcccagat cctcttcgaa gacttcctgg caagtggtgc gactttcggc gtcggacatc
     1681 gactcgaaac ctgccctcga ccaggtcgcc gcgtcgcatt cgacgataaa cgtgctcgtc
     1741 gtctggttgt agcgatacgc gtgcgcaatg aagatgccat gggcatgttc gcggaagatc
     1801 agggtcagcg cgggaaatac ctggtgcgtc ccgtaccaga cgtatcggtt gggccgcacg
     1861 tcaagcgacg gctgaaagcg ctcgcgatga gcttgccgga cgacgctgtt cacgccgtct
     1921 gcgccgacga gcagatcgca gtccgccaaa gacgctgcat cggtgactcg ccgctcgaac
     1981 tgcatgtcga cgcccagacg ccggcagtgc tcttgtaaaa tgcgcagcag ttcgaggcga
     2041 gcgattccag aataggtgtt gccctcaatg gctgtacgtc gtcccttgtg gacgatggcc
     2101 agatcgtccc agatttcgag gccggctacc acgtccgcgt aaaagtcggc atcgccgtcg
     2161 cgcaggtagg ccagagcccg atccgaaaac acaacgcccc agccgtacgt cgcatcggcg
     2221 gggtcctgct cgacgacctg gaccttatgc gccgggaagc gtttcttcat caacagcgca
     2281 aaatacagtc cggccggccc gccgccgacg ataccgatct tcacggagcg tcacctgtca
     2341 acaacgccgt cgcctgctcc acatgcacct gatcgtgctc aactacgaaa cgctcgatga
     2401 actcggacag catttgcggg ccgtacttga cgtgctcgcc ctgaatccgc aaatccgcgt
     2461 tggtcatctc acccaatgtt ttcaggaggc tctcgtggct ggcgatgagt ctgctgcgaa
     2521 gggcgttcgg agcgtcgtgg ccgtgatcct cgatgttttg caaccggccg gggtgctcga
     2581 ccgtgcgccc catcttcgtg cccggcgccg cccgcacccg gcgtgtcgcc tcgctgaaga
     2641 actggcgcgc ctcggcgatg tgcgccagcg tctcggccag tgtccacccg ccgtcggccg
     2701 gacggcgata gaggatgtgc tcgtcggcgc gactcaggat gtcgagaaaa acctgctgcg
     2761 tcaggcgcag gtcgtccagc atctcagcca gcgttcgttc agccatattc atttctccgc
     2821 ccgaagacac ccgtcacagt ctgatggccg tcgtcctcga cgtaggcctc ctctcgatac
     2881 ttgttcagcg caaccatcac cggcgtgtcc tggatcgaaa agagaaccgg cgatggcgct
     2941 tctctcttcc gttcatgttc cgaggggccg cgagccgatc cgcctggagc ctttgcgggc
     3001 agtcacaccg gcaagtcagt cttgacaaag tgagccggga atgttatatt aaaaacatac
     3061 cacggagtat gttttttgtc aagcatgtac gcgaagtccg gcaccaccat cgccgccatc
     3121 ctgggcgcgg ccgagaaact cttcctggcg cgaaactacg ccaacgtcgc gatggacgac
     3181 atcgccgagg cggcgggtgt caccaaaggc gcactctacc accacttcag gagcaagaaa
     3241 gacctgtacc tggcgatgat gcacagcgac ctgaagcaga aacaggcgcg catgcgcgcc
     3301 ggcgtggaga tgaagggaag ctgttacgag cggctacggc ggctgacgga gagcttcctc
     3361 gaacttccac ccgaaaagcg tgatttgatc aagctcgttc gccgcgacat caacatcttc
     3421 aaaggcccgg cgcgcgaccg gctggtgcga gcgtaccagg ccgctttgcc ggatcaggtg
     3481 gaggttatcg ttcgcgatgg cattcggaag ggtgaactgg ccgacgccga tccgcgcgtg
     3541 ctggcctgga tgttcgtcgc gatcgtcgaa gttgcgctga caccttacgc cgggcacgtg
     3601 ctgagggacc cgtccacgat gctggacttt gtgatggaca tgttcttcgc aggcgcaggc
     3661 cggccagcgc gccacgcgat atcgaggatg gaacggcgcg ccgcgcgcgt gtccgttgcg
     3721 gcataacgac taccggcact cgagatccgc tcttacaccc gagggaggca atatccatat
     3781 ggcaaaaatg ttcatcaacg ggcaatgggc cgacgccctg cgcggccaga cggtcgaggt
     3841 caagaatccc gccaacggca ctctcgtcga cacggcgccg ctcgccggga aggaagacgt
     3901 cgaggcggcg atcaaggcgg cgcagacggc ttttccggga tgggcggata cctcgcccga
     3961 cgatcgcgcc gcgatcctgc gcaaaggcat tcaggccgtc gaggggcatg cccaggagat
     4021 cgcggcgctg ctcaccgccg aacagggcaa gccgctcttt gaagcgtccg gcgagcttca
     4081 cc
//
LOCUS       DBNHXB010000162        14441 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_204882, whole genome shotgun sequence.
ACCESSION   DBNHXB010000162 DBNHXB010000000
VERSION     DBNHXB010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 14441)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14441)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14441
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_204882"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="AC162G_06320"
     CDS             complement(<1..1358)
                     /locus_tag="AC162G_06320"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR5075460.1"
                     /translation="MLKPSLTTNMTAVRERIVGVRQRVPTLNGETRTYVNFDNSASTP
                     VLQDVLDTVNTFMHWYASVHRGTGFKSRVATQAYDDAREIVARFVGANPREHVVIFGK
                     NTSEAINKLARRIPLAKDDVVLVSQLEHHSNDLPWRAVATVDHVAADALGDLDEGDFD
                     HKLDRHRGRLKLVAITGASNVTGFLPPIHHLAEKAHAAGAQILVDCAQLAPHRPISIG
                     TLDDPAHLDYVALSAHKMYAPFGTGALIGRRDTFETGAPDYSGGGTIEIVTLDSVEWS
                     APPERDEAGSPNVVGAVALAAAVKALQSIGMTAIADHEAELTAYTLERLQSIDGIRIY
                     GDPDPTSTSSRLGVIPFNVDGMSHFKVAAILGAEFGIGVRNGCFCAHPYLLHLLSLSH
                     EESESVRHNILADDRREMPGMVRVSFGMYNTAEEVDVFVDAVGRIARGKVYGRYEQDA
                     RSG"
     gene            complement(1362..2324)
                     /gene="era"
                     /locus_tag="AC162G_06325"
     CDS             complement(1362..2324)
                     /gene="era"
                     /locus_tag="AC162G_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR5075461.1"
                     /translation="MTEPEALSLAEFEDTPPGHRSGFVAVVGKPNVGKSTLMNALLGE
                     KVAIVSKKPQTTRAQIRGILTRPDAQIVFVDTPGIHKPRHRLGQAMVQAAIQSIPDAD
                     IVLFVVDSTEAPDAGDRHIARLVRERGEDRPVILALNKMDRLRPDQVKAHTEAYWSLA
                     PTPGGGAPPEPWDWMMISATQLVNLDKLVDRIVARLPEGPRYYPPDQITDQDERYTAA
                     ESIREQALRHLRREVPHAVAVVVEEHEERSATLDYIAATIVVEKDSQKGILIGKRGHT
                     LRQIGADARHEIERLLGKQVYLDLQVKVRKNWREDERELRRMGY"
     gene            complement(2321..3682)
                     /gene="tyrS"
                     /locus_tag="AC162G_06330"
     CDS             complement(2321..3682)
                     /gene="tyrS"
                     /locus_tag="AC162G_06330"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR5075462.1"
                     /translation="MDGQRINEYKLCAERVGALGTCPDQALAEWYNQQHNTHTWHAGY
                     AMPSIDEQVAVLMQGAEYGDETLKQNMAGELRQRLIESQREGRPLRVYCGYDPTKPDL
                     HLGHTITMRKLRQFQDLGHDVTFLIGDYTTLIGDPSDKDKLRPILSPAEIRSNAETYA
                     DQAFKILDRKKTRIRYNSEWLSQLTFGDVIKLASNFTVQQFMARENFALRLERGDPVY
                     LHETLYGLMQGYDAVAQQTDVQVGGSEQLFNIIVAGRKLQEAMGQKPQIGILMDILPG
                     TDGVVRMSKSQGNTIEIMAPPEDMYGKVMSLPDSAMPIFYKLVTRFTPAQIAGIEKGL
                     EDGSLHPRDVKMQLAREVVSIFHDEAAAARAEEHFVAVFRQRELPPEMPEMRLDQPAN
                     IVDLIASARLASSKSEARRLIEQGGVRLDGVAVSDIALVVEPGKETVLQVGKRKYLKL
                     LPK"
     gene            3753..6644
                     /gene="alaS"
                     /locus_tag="AC162G_06335"
     CDS             3753..6644
                     /gene="alaS"
                     /locus_tag="AC162G_06335"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR5075463.1"
                     /translation="MTKKAGMTKKKTAGKKPAVARATPPRAPRESKSTTAPLERPQLT
                     SAEIRQTFLGFFAEHGHTIVPSTSLIPADDPTLLFTNSGMVQFKDVFLGLGKRPYTRV
                     ADSQKCMRVAGKHNDLDDVGRDGSHHTFFEMLGNWSFGDYYKKEAIAWAWQLFTDVWS
                     LPKERLWATCFEDEQGVIPRDDEAADIWRSQPGFDPSHVLFFGRKENFWEMADTGPCG
                     PDSEIHFDRGIEFCDKQGVPGHVCRVSGDCPRFLELWNLVFIQYNRTGPTTLEPLPAR
                     HVDTGMGFERIVSVLQNAKTNYDTDLFMPLIRRTQELTGHTDAEVRHNIVAYRVIADH
                     GRGAAFLVADGVLPGNVGRSYVLRMIIRRAARFGRKIGLDFPFLAEIADVVIGEMGDH
                     YRELRDRSEHILHTLTDEEERFQRTLDLGLSKLEDALAEAKNRIIPGEAAFRLYDTFG
                     LPLEITKDVALERGYAVDEAGYQKALAEQRERARAGEKFEMDDQSLANYHRVLRTLQD
                     GGKLGGAGVEHDPYTAPEMETNVISLLVDGQPVDQAKPGDVVEVILPATPFYVESGGQ
                     ISDTGVIARFPEQGSGEPLWEITVADTRRPIAGLIVHAGKVKSGRPRAGDPAWALVDY
                     ERRWDIMRNHTATHLLHAELRYVLGTHVHQAGSLVAPDRLRFDFTHSTMLTQDELDAI
                     VENVNGAILANYPVVPTLRPYAEAVADGAMALFGEKYGDIVRVVRTGWPDEPPISIEL
                     CGGTHVNQTAEIGSFVLASEGSVGAGVRRIEALTGRGAQQYVRERLNKLTAAAAYLNA
                     SPEEVDRKVLALLEENQTLQKHLDKMRRESAQREVDALLSRVEYVDDVAVLSAQVKAD
                     TTDMLREMTDWFRDRLKSGVVVLGAVMNKRPSLIAAVTPDLIERGLDAGQIVRQAARV
                     IGGGGGGKPTLAEAGGKDASKLSEALASVRHIVAESVLEKPGFLEKPGF"
     gene            6664..8268
                     /locus_tag="AC162G_06340"
     CDS             6664..8268
                     /locus_tag="AC162G_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HIR5075464.1"
                     /translation="MANLFARSMLVLVLLFGLLFAVGMVVLTAIDAPVWMAVVFGLGI
                     LFLQYLLGPWILQLIYKIEWRDPQSIDPKLAEFVGRVCAERRIPIPRFGVIHDGNPTA
                     FTFGHYPGDARLVVTTGLLDLLDSEEYQAVVAHELGHIAHWDFVVMTIAAGIPLVLYI
                     LFRVTLSGRGRGKSGGYLALIGIISYVAYLVSHFIALLLSRVREYYADQFAGEVTGNP
                     NALSSALVKVAYGLAAAPKDKKKDDMRMTAARAFGIFDPRAAQSLALAGAGSGSVSID
                     SMENAMKWDLWNPWAKLFELGSTHPLPAKRIRALERQSEANGQLPRFSFRAERPESYW
                     DEFLVDVLINYLPLIALVVALAWVAISAYSLRFSVGALGLALLLVSLTWWLKRRFSYR
                     HRFDERRAVSSLIGEVKVSAVRPIPCTLEGQIIGRGVPGLFYSEDLVLQDDTGYIVVD
                     YRQPLGILEFLFGWLKAGELIGRRGKVMGWYRRSPRPYIELRKMMLEDGKTITSYSYP
                     VAQFLVYAGILLGVILLIAQFTLRTF"
     gene            8318..9844
                     /locus_tag="AC162G_06345"
     CDS             8318..9844
                     /locus_tag="AC162G_06345"
                     /inference="COORDINATES: protein motif:HMM:NF016736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HIR5075465.1"
                     /translation="MEQILELHEHEDVHSIRDRLEHAQADRALLVVPPYSDVLKKRVD
                     LQLIRRFAQQKHVEIALVTHDMQQRALAAELGVPTFLTVEAGQSRQRWRRPVDDSFGP
                     YRPPDRSEAHQAAQDRDARIAPSPLRDRIWPGVKAALILIVAVAIVVSVVAVLPSAEV
                     YLVPQSERVVTNVSIVADAGIEAVDVAGGHVPVTRYVTDVKGEITIPTSGRKFIPTSR
                     ATGVVFFVNQLNIPFRVQKGTVVRTSAGAQAIRFVVTSDVEIPGGIGAQAQAPIEAVE
                     VGAQGDVLPNFINEIEGVAALSLRVSNPDATRGGGDREVSAVDPQDLVTAREQLLEQM
                     RQQALDQLAAENLDETEFLLNSSLSIEEILDATYDKEVTEQATELTLQMRARFSALAV
                     NSEDANAIVYAAMRNQAPIGYDLLADGLAFQRGAEEPAGDAGQYRFEMQGIGYAAADL
                     DLGGAIEAIAGKPAEVAAEILQSSLPLQKPPRITVSPSWFPVVPLMPVRVRTIVDASG
                     "
     gene            9845..10288
                     /gene="ruvX"
                     /locus_tag="AC162G_06350"
     CDS             9845..10288
                     /gene="ruvX"
                     /locus_tag="AC162G_06350"
                     /inference="COORDINATES: protein motif:HMM:NF015607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR5075466.1"
                     /translation="MARLLALDIGDRRIGVAISDESQMLARPLTTIVRASKREDFESI
                     AKLIAAHGVERLIVGLPRTLRGDEGAQAARVRRYAEALSAAIGAPIAFQDERYTSLEA
                     EERLAASPRRKRAGGDVDAAAAAIILQDYLNQHTSPPPPGEAGEG"
     gene            10308..11435
                     /gene="mltG"
                     /locus_tag="AC162G_06355"
     CDS             10308..11435
                     /gene="mltG"
                     /locus_tag="AC162G_06355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR5075467.1"
                     /translation="MKRRTRSLLIFSITLVMLGAAGAIAYQRLRSGMTDLQLEAPEDV
                     QDALIAAYLSANQEKLEQPAGSDPTPIEFEIEAGESLNAIANRLAQAGLIGDTELFRR
                     YLQYNRLDQGIEAGNFTLNKAMTIPQIAQALQEGRREELTLTVPEGKRLEEVAQIVGS
                     QIPQIDANEFSLLASDASLWKSQFSFLSDIPEGGSLEGYLFPDTYRLPLETDARDVMT
                     RMLRNFDAQVTAQMRADAQASGRSLWDVIRLASIVEREAVVAEERPLIAGVYLNRLAQ
                     GMALDADPTIQYALGQSREPGNWWPQLTLEDYQGVAAPYNTYLNPGLPPGPIASAGRG
                     SIEAVIYPEQTDFFFFRASCSRDGTHQFARTLEEHAANECP"
     gene            11595..12386
                     /locus_tag="AC162G_06360"
     CDS             11595..12386
                     /locus_tag="AC162G_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5075468.1"
                     /translation="MKLSVSLFAFLTFALAACSTGSTAPVVKIGLAAPFEGRYRAIGY
                     EAIYAARLAIREINAAGGIDGYRVELSALDDGGDPAMAIEQARKMAIDPQVIAVIGHY
                     RPETTGAATGHYCAERLPLLAVEPVRTSVCHEVLALTPPITTTLSSSDPASFLFAGTV
                     PHPADVPEAAAFVEAYNAIPIDGTRAGPIALQTYDAMYLLFDALRRNLQAEGRPSRDG
                     MARALAESDFTGLGGVYRFDAEGNRTQMKTYTYRYSKDREPELIR"
     gene            complement(12429..12551)
                     /locus_tag="AC162G_06365"
     CDS             complement(12429..12551)
                     /locus_tag="AC162G_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075469.1"
                     /translation="MNPYVVAGAVALFAAIAWYVITSRRMLAKLPPRDKDKVKK"
     gene            complement(12569..14359)
                     /locus_tag="AC162G_06370"
     CDS             complement(12569..14359)
                     /locus_tag="AC162G_06370"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HIR5075470.1"
                     /translation="MNEPHPLSDDRRAELDEAAIVFLTLHNVERPPVPIEEILRNPVA
                     EMWQPDLVDLSHQSFEATDRYSVRPTIARVVARYVSGTRWARERGIAGNAGFTADEAR
                     YFAHALLMPKEWIGALPASERTPASVRLRFHVPLADAAGRLAELDSQPNGSLGSSNPG
                     GSMRAVDVIEKKRDGHELTREEIDFFVQGYTRGDIPDYQAAAWCMAVLLRGMTARETT
                     DLTLAMARSGKTLDLHDIAPFVVDKHSSGGVGDKVSLVVAPLVASSGLPVGKMSGRGL
                     SFSGGTLDKLESIPGFRTNLSEDEFRAQLKRAGIVLTGQSVDLAPADGKLYALRDVTG
                     TVPSLPLIAASIMSKKIAGGADGIVLDVKCGSGAFMKTEADARVLAGIMVDIGKMLGR
                     RTVALISDMNQPLGSAVGNALELREAIKTLRGRGPSDFAEHCLTVAAHMLRLGAKAAS
                     VEEGRARAGSLIHEGRAWRKFVELVTVQGGDASVVEQPDHLPRATIVCDVPSPRGGYV
                     ARVDALEIGLSAVMLGAGREKKGDPIDHAVGVEVHVKVGDRIDPGARLFTVHANDEHR
                     RVEAETRLLGAVTFSDARVEPLPLFYGVIE"
BASE COUNT         2476 a         4681 c         4740 g         2544 t
ORIGIN      
        1 cccgaccggg cgtcctgctc gtagcggccg tagacctttc cgcgcgcgat gcgccccacg
       61 gcgtcgacga acacgtcgac ctcttcggcg gtgttataca tgccgaagga tacgcgcacc
      121 atgccgggca tctcgcgccg gtcgtcagcc aggatgttgt gacggacgga ctcggattcc
      181 tcatgcgaga ggctgagcag gtgcagcaga tacggatgcg cgcagaagca gccgttgcgc
      241 acgccgatgc cgaattctgc gccgagaata gccgcgactt tgaagtgcga catgccgtcg
      301 acgttgaacg ggatcacgcc gagacggctg ctcgtcgaag tcgggtcggg atcgccatag
      361 atgcggatac cgtcgatcga ctgcaggcgc tctaacgtat aagccgtcag ctcggcttcg
      421 tgatcggcga tggccgtcat gccgatggat tgcagcgcct tgacggcggc ggccagcgcg
      481 accgcgccga cgacgttggg agacccggcc tcgtcgcgct cgggaggggc cgaccactcg
      541 accgaatcga gggtgacgat ttcgatcgtc ccgccgccgc tgtagtcggg cgcgccggtc
      601 tcgaaggtgt cgcgccggcc gatcagcgcg ccggttccga acggcgcgta catcttgtgc
      661 gcggagagcg ccacgtagtc gaggtgcgcc gggtcgtcga gcgtgccgat cgagataggg
      721 cgatgcggcg cgagctgtgc gcagtccacg agaatctgcg cgccggcggc gtgcgctttc
      781 tcggctaggt ggtggatcgg cggcaggaag ccggtgacgt tcgacgcgcc ggttatggcg
      841 accagcttca ggcgtccccg gtgccgatcc agcttgtgat cgaagtcgcc ctcgtcgaga
      901 tcgcccaacg cgtcggcggc gacgtgatcg accgtcgcaa cggcacgcca gggcagatcg
      961 ttgctgtggt gctcgagctg cgagaccagc acgacgtcgt ccttcgccag cgggatgcgc
     1021 cgggccagct tgttgatcgc ttcgctggtg ttcttgccga agatcaccac gtgctcgcgc
     1081 ggattcgcgc ccacgaagcg ggcgacgatc tcgcgtgcgt cgtcgtacgc ctgcgtcgct
     1141 acgcggcttt tgaagcccgt gccgcgatgg acgctcgcat accagtgcat gaaggtgttg
     1201 acggtgtcga gcacgtcctg caagaccggc gtggacgcgg agttgtcaaa gttgacgtac
     1261 gtgcgcgttt cgccgttgag cgtcggcacg cgctgcctga cgccgacgat ccgctcgcgc
     1321 acggcggtca tgttggtggt gagtgacggt ttgagcatag cctaataccc cattcgcctc
     1381 aactcgcgct cgtcctcgcg ccagttcttg cgcactttca cctgcagatc gagatagacc
     1441 tgcttgccta acagccgctc gatctcgtga cgcgcgtccg cgccgatttg ccgcagcgtg
     1501 tgcccacgct tgccgatcaa gatgcctttc tgcgagtctt tctcgacgac gatcgtcgcg
     1561 gcgatgtagt ccagcgtcgc cgagcgctcc tcgtgctcct cgaccacgac ggcgacggcg
     1621 tgtggcacct cccgccgtaa gtgacgcagc gcctgctcgc ggatcgactc ggcggcggtg
     1681 tagcgctcat cttgatcggt gatctgatcg ggcggatagt agcgcggccc ctcgggcagg
     1741 cgggcgacga ttcgatcgac gagtttgtcg agattgacga gctgcgtcgc cgagatcatc
     1801 atccagtccc acggctcagg cggcgcgccg cccccgggtg tcggcgcgag cgaccagtaa
     1861 gcttcggtgt gcgccttgac ctggtcgggc cgcaggcgat ccattttgtt gagggccagg
     1921 atcaccggtc gatcttcacc gcgctcgcga acgagccggg cgatgtgccg atcgccggcg
     1981 tcgggcgctt cggtcgaatc gacgacgaac aaaacgatgt cggcgtccgg gatcgactgg
     2041 atggccgcct gcaccatcgc ctggccgagc cggtggcgcg gcttgtggat gccgggcgta
     2101 tcgacgaaga cgatctgcgc atcgggccgc gtgagaatgc cgcggatctg tgcgcgcgtc
     2161 gtctgcggct tcttgctgac gatggcgacc ttctcgccaa gcagcgcgtt catcagcgtc
     2221 gacttgccga cgttcggctt gcctaccacg gcgacgaacc cgctgcgatg gccgggcggc
     2281 gtgtcttcga attctgccaa cgagagggct tcaggttcgg tcatttcggc aacaacttga
     2341 gatacttgcg tttaccgact tgcagcacgg tttctttgcc cggctcgacg acgagcgcga
     2401 tgtcgctcac cgcgacgccg tccaggcgga cgccgccctg ctcgatcagc cggcgcgcct
     2461 cgcttttgct cgaagcgagc ctggccgacg caatcagatc gacgatgttc gccggctgat
     2521 ccagtctcat ctcgggcatc tccggcggca gctctctctg ccggaacacg gcgacgaagt
     2581 gctcctcggc gcgcgcagcg gcggcttcgt cgtggaagat cgagacgacc tcgcgggcaa
     2641 gctgcatctt gacgtcgcgc ggatgcaacg agccatcttc caggcccttc tcgatccctg
     2701 cgatttgcgc cggcgtgaag cgcgtgacca gcttgtagaa gatcggcatc gccgaatcgg
     2761 gcaggctcat caccttgccg tacatgtcct ccggcggggc catgatctcg atggtgttgc
     2821 cctggctctt ggacatgcgc accacgccgt cggtgccggg caggatgtcc atcaagattc
     2881 cgatttgcgg cttctggccc atcgcctcct gcagcttgcg cccggcgacg atgatgttga
     2941 agagctgctc gctgccgccc acctgcacgt cggtctgttg cgcgaccgcg tcgtagccct
     3001 gcatcaggcc gtacagcgtc tcgtgtaggt acaccgggtc gccgcgctcc agccgcagcg
     3061 cgaagttctc acgcgccatg aactgctgga cggtgaagtt ggacgccagc ttgatgacgt
     3121 cgccgaacgt caactggctg agccactcgg agttgtagcg gatgcgggtc ttcttccgat
     3181 ccaggatttt gaatgcctga tcggcgtacg tctcggcgtt cgagcggatc tcggccggcg
     3241 acagtatcgg gcgcagcttg tccttgtccg acggatcgcc gatcagcgta gtgtagtcgc
     3301 cgatcaggaa cgtcacgtcg tggccgaggt cctgaaactg gcgcagcttg cgcatggtga
     3361 tggtatggcc caggtgcaga tcaggcttgg ttgggtcata gccacagtac acgcgcagcg
     3421 ggcgaccctc ccgttggctc tcgatcagcc gctggcgaag ctcgccggcc atgttctgtt
     3481 tgagtgtctc gtcgccgtac tcggcgccct gcatcaggac ggcgacctgc tcgtcgatgg
     3541 acggcatggc gtaccccgcg tgccaggtgt gagtgttgtg ctgctgatta taccattcag
     3601 ccagcgcttg gtcggggcac gtcccaagcg cgccgacccg ctccgcgcac agtttgtatt
     3661 cgtttatccg ttgcccatcc attcacgcct ttgacagttg acagtccctg cgatacaatc
     3721 tcaaaccacc tacggttctt ttaggggagt ccatgacgaa gaaagcaggg atgacgaaga
     3781 agaagaccgc cgggaagaag ccggctgtcg cccgggcgac gcctccgcgc gcgccgcgcg
     3841 aatcgaaatc cacgacagcg ccgctcgagc gcccgcagct cacgagtgcc gagatccggc
     3901 agaccttcct cggcttcttc gccgagcacg ggcacactat cgtgccgtcc acgtcgctga
     3961 tcccggccga cgatccgacg ctgctgttca ccaactcggg catggtgcaa ttcaaggacg
     4021 tgttcctcgg ccttggcaag cgcccgtaca cgcgcgtcgc agactcccag aaatgcatgc
     4081 gcgttgccgg caagcacaac gacctcgacg acgtgggccg cgatggatcg caccacacct
     4141 tcttcgagat gctcggcaac tggtcgttcg gcgattacta caagaaagag gccatcgcct
     4201 gggcctggca actgttcacc gacgtgtgga gcctgcccaa ggaacgactg tgggcgacgt
     4261 gcttcgagga cgagcagggc gtgattccac gcgacgacga ggccgccgac atctggcgca
     4321 gccagcccgg cttcgatccg tcgcacgtcc tgttcttcgg ccgcaaggaa aacttctggg
     4381 agatggccga caccggcccc tgcggccccg acagcgagat ccacttcgat cgcgggatcg
     4441 agttctgcga caagcagggc gtgccgggcc acgtctgtcg cgtgagcgga gactgcccgc
     4501 gcttcctcga gctgtggaac ctggtgttca tccagtacaa ccggaccgga ccgacgacgc
     4561 tcgagccgct gccagccagg cacgtcgaca cgggcatggg cttcgagcgc atcgtgtcgg
     4621 tgctgcagaa cgcgaagacc aattacgaca cggacctgtt catgccgctg attcggcgca
     4681 cgcaggaact gaccggtcac accgacgccg aagttcggca caacatcgtc gcgtaccgcg
     4741 tgatcgccga tcacggccgc ggcgcggcgt tcctcgtcgc cgacggcgtg ctgcccggca
     4801 acgtcgggcg cagctacgtc ctgcgcatga ttattcgccg cgcggcgcgc ttcgggcgga
     4861 agatcgggct ggatttcccg ttcctggccg agatcgccga cgtggtgatc ggcgagatgg
     4921 gcgatcatta ccgggagctg cgcgatcgga gcgagcacat cctgcacaca ctgaccgacg
     4981 aggaggagcg cttccagcgc acgctcgacc tgggcctgtc gaaactggag gacgcgctgg
     5041 ccgaggcgaa gaatcgcatc atccccggcg aggcggcctt ccgcctctac gacaccttcg
     5101 gcctgccgct cgagatcacc aaagacgtgg cgctcgagcg cggctatgcc gtcgacgagg
     5161 ccggctatca gaaagcgttg gccgagcagc gcgaacgcgc gcgcgccggc gagaagttcg
     5221 agatggacga ccagtcgctg gcgaactacc atcgcgtgct gcgcacgctg caagacggcg
     5281 gcaagctggg cggggcgggc gtcgagcacg atccctacac ggcgcccgag atggaaacca
     5341 acgtgatcag cctgctggtc gacggtcaac cggtcgatca ggccaaaccg ggagacgtcg
     5401 tcgaagtcat cttgcccgca acgccgttct acgtcgagtc gggcggccag atcagcgaca
     5461 ccggcgtgat cgcccgcttt cctgagcagg ggtcgggcga gccgctgtgg gagatcaccg
     5521 tcgccgacac gcgccggcca attgccggcc tgatcgtcca cgcgggcaag gtgaaatcgg
     5581 gccggccgcg cgccggcgac ccggcatggg cgctggtcga ttacgagcgg cgctgggaca
     5641 ttatgcgcaa ccacacggcg acgcacctgc tccacgccga gctgcgctac gtgctgggga
     5701 cgcacgtcca ccaggccgga tcgctcgtcg cgcccgatcg gctgcgcttc gacttcacgc
     5761 acagcacgat gttgacgcag gacgagctgg atgcgatcgt cgagaacgtc aacggcgcga
     5821 ttctggcgaa ctatcccgtc gtcccgacgc tccggccgta cgcggaggcc gtcgccgacg
     5881 gcgcgatggc cttgttcggc gagaaatatg gcgacatcgt gcgcgtggtg cgcaccggct
     5941 ggccggacga gccgcctatc agcatcgagc tgtgcggcgg cacgcacgtc aaccagacgg
     6001 ccgagatcgg gtcgttcgtc ctggcatcgg agggcagcgt gggcgcgggc gtgcgccgca
     6061 tcgaggcgct gaccggccgc ggcgcccagc agtacgtgcg tgagcgcctg aacaagctga
     6121 ccgcggcggc ggcttatctc aacgcgtcgc ccgaagaagt cgatcgcaag gtgctggcgc
     6181 tgctcgaaga gaaccagacc cttcagaagc atctggacaa gatgcggcgc gagtcggcgc
     6241 agcgcgaggt cgatgcgctg ctgagccggg tcgagtacgt cgacgacgtg gccgtgctgt
     6301 cggcgcaggt gaaagccgac acgaccgaca tgctccgcga aatgaccgac tggttccgcg
     6361 atcgcctgaa gagcggcgtc gtggtactgg gcgcggtcat gaacaagcgc cccagcctga
     6421 tcgcggccgt cacgcccgat ctgatcgagc gcggcctgga cgcgggccag atcgtgcgcc
     6481 aggcggcgag agtgatcggc ggcggcggcg gcggcaagcc gacgctcgcc gaagccggcg
     6541 ggaaagacgc ctcgaagttg tccgaggcac tggccagcgt gcggcacatc gtggcagagt
     6601 cggtactcga gaagccgggc ttcttggaga aacctggttt ctgaacaagt aaggaggcgc
     6661 gacatggcta atctgttcgc tcgttccatg ctcgtgctgg ttttgctctt cggcctgtta
     6721 ttcgccgtgg gcatggtcgt cctgaccgcg atcgacgctc cggtgtggat ggcggtcgtc
     6781 ttcgggctgg gcatcctctt cctgcagtac ctgcttggcc cgtggatttt gcaactgatc
     6841 tacaagatcg agtggcgcga tccgcagtcg atcgacccga aactagccga gttcgtcggc
     6901 cgggtgtgcg ctgagcgcag gattcccatc ccacgcttcg gcgtgatcca cgacggcaac
     6961 ccgaccgcct tcacattcgg ccattacccc ggcgatgcgc gcctggtagt gaccaccggc
     7021 ttgctggacc tgctggatag cgaggaatat caggccgtcg tcgcgcacga gctgggccac
     7081 atcgctcact gggatttcgt ggtgatgacg atagccgccg ggattcccct ggtgctgtac
     7141 atcctcttcc gggtgacgct ctcgggacgg ggacgtggca agagcggcgg ctacctcgcg
     7201 ctgatcggca tcatctcgta cgttgcctat ctcgtcagcc acttcatcgc gctgctgctg
     7261 tcgcgcgtgc gcgaatacta cgccgaccag ttcgccggcg aggtgacggg caaccccaac
     7321 gcgctgtcga gcgcgctggt caaggtcgcc tacggtctgg ccgccgcgcc gaaggacaag
     7381 aagaaagacg acatgcggat gacagcggcg cgcgccttcg gcattttcga tccgcgcgcg
     7441 gcgcagagcc tggcgcttgc cggcgcgggc agcggctcgg tctccatcga ttcgatggag
     7501 aatgcgatga agtgggatct gtggaacccg tgggccaagc tcttcgagct aggctcgact
     7561 catccgctgc cggccaagcg catccgcgcg ttggagcgcc agagcgaggc gaacggccag
     7621 ttgccgcgat tctctttccg cgccgaacgg ccggagagtt attgggacga gttcctggtc
     7681 gacgtcttga tcaactacct gccgctgatc gcgcttgtgg tggcgctcgc gtgggtagcc
     7741 atctcggctt attcgctgcg ctttagcgtc ggcgccctgg ggcttgcgct gctcctcgtc
     7801 agtctgacct ggtggctgaa acggcgcttt tcttacagac accggttcga cgagcggcgt
     7861 gcagtgtcgt cgctcatcgg cgaggtgaaa gtttctgccg tgcgcccgat cccctgcacg
     7921 cttgagggac agatcatcgg gcggggcgtg cctggcttgt tctacagcga agacctggtg
     7981 cttcaggacg acacggggta catcgtcgtc gactaccgcc agccattggg cattctcgag
     8041 ttccttttcg gctggctcaa ggcgggagag ctgatcggtc ggcgaggaaa ggtgatgggg
     8101 tggtaccgcc gctcaccccg tccttacatc gagctgcgca agatgatgct ggaggatggc
     8161 aagacgatca ccagctattc ctacccggtc gcacagttcc ttgtgtatgc aggcatcctg
     8221 ctgggcgtca ttttgctgat cgcacagttc acgcttcgga ctttctgagc agtctcagta
     8281 gatccaaatt cggaagcaga gcgggctccc acgtctcatg gaacaaatcc tcgagttgca
     8341 cgagcacgaa gacgtccaca gcatccgcga ccggctggag cacgctcagg ccgatcgggc
     8401 cttgctggtc gtcccgccgt acagcgacgt gctgaaaaag cgcgtggact tgcagctcat
     8461 ccggcgcttc gctcagcaga agcacgtcga gatcgcgctg gtcacgcacg acatgcagca
     8521 gcgcgcgctc gcagccgagc tgggcgttcc tacattcctc accgtcgaag ccggccaaag
     8581 ccggcaacgc tggcgcaggc cggtcgacga cagcttcggc ccgtatcgac cgcccgatcg
     8641 cagcgaagcg caccaggccg cccaggatcg cgacgcgcgc atcgccccga gtccgctgcg
     8701 agatcggatc tggccgggcg tcaaagcggc gctaatcctg atcgtcgccg tcgcgatcgt
     8761 cgtcagcgtc gtcgccgtgc tgcccagcgc cgaggtgtat ctggtcccgc agtcggagcg
     8821 cgtcgtgacg aacgtgtcga tcgtcgccga tgccgggatc gaggccgtcg acgtcgcggg
     8881 gggccatgtg cccgtcaccc gatacgtgac cgacgtgaaa ggcgagatca cgataccgac
     8941 gagcgggcgg aagttcattc ccacgtcgcg ggcgacaggc gtggtgttct tcgtcaatca
     9001 gctcaacatt cccttccgcg tgcagaaggg cacggtcgtg cgcacatcgg cgggagcgca
     9061 ggccattcgc ttcgtcgtca cgtctgatgt cgagattccc ggcggcatcg gcgcgcaggc
     9121 gcaggcgccg atcgaggcgg tcgaggtcgg ggcgcagggc gacgtgctgc ccaacttcat
     9181 caatgagatc gaaggcgtgg ccgcgttgtc actgcgcgtg agcaatcccg acgcgaccag
     9241 gggcggcggc gatcgcgaag tcagcgccgt cgatccgcaa gacctggtga cggcgcgcga
     9301 gcagctcctg gaacaaatgc ggcaacaggc gctcgaccag ctcgcggcgg agaacctgga
     9361 cgagaccgaa ttcctgctga acagctctct gtcgatcgag gagattctcg acgcgacgta
     9421 cgacaaggaa gtgaccgagc aggcgacgga actgacgctg cagatgcgcg cgcgattcag
     9481 cgcgctggcc gtcaacagcg aggacgccaa cgcgatcgtg tacgcggcga tgcgcaacca
     9541 ggcgccgatc ggctacgatc tgctcgccga cgggctggcg ttccagcgcg gcgccgaaga
     9601 gccggccggt gacgcgggcc agtatcggtt cgagatgcaa ggcatcggct acgcggcggc
     9661 cgatctcgac ctgggcggcg cgatcgaggc catcgccggc aagcccgccg aagttgccgc
     9721 cgagattctt cagtcgagcc tgcctctgca gaagcccccc cgcatcacgg tctctccgag
     9781 ttggttcccg gtcgtcccgc tcatgccggt gcgcgtgcgc acaatcgtcg acgcatcggg
     9841 gtaaatggca cgcttgctgg ctttggacat tggcgacagg cgcatcggcg tggccatcag
     9901 cgacgagtca cagatgctgg cccgcccgct gacgacgatc gtgcgcgcgt cgaagcgcga
     9961 ggacttcgaa tcgatcgcga agctgatcgc ggcgcacggc gtggagcgcc tgatcgtcgg
    10021 cttgccgcgc acgctgcggg gcgacgaggg ggcgcaagcc gcccgcgtgc gccgctacgc
    10081 cgaagcgctg tcggcggcga tcggcgcgcc gatcgcgttt caggacgagc gttacacgtc
    10141 gctcgaggcc gaggagcgcc tggccgcatc gccgcgccgg aagcgcgccg ggggcgacgt
    10201 cgacgcagcc gcggcggcga tcatcttgca ggattatctc aaccaacaca cctcaccccc
    10261 gccccctggc gaagccgggg aggggtgagg tcgggagaac tggcatcttg aaacgccgca
    10321 cccgatcgct ccttatcttt tcgatcacgc tcgtcatgct cggcgcggcg ggcgcgatcg
    10381 cttaccagcg cctgcgcagc ggcatgaccg atcttcagct cgaggcaccg gaagatgtgc
    10441 aggacgcgct gatcgccgcg tacctcagcg ctaaccagga gaagctggag cagcccgccg
    10501 gcagcgatcc gacgccgatc gagttcgaga tcgaggccgg cgagagcctg aacgccattg
    10561 ccaaccggct ggcgcaggcc ggcttgatcg gcgacacgga gctattccgg cgttacctcc
    10621 aatacaaccg gctcgaccag ggcatcgagg cgggcaactt cacgctgaac aaggcgatga
    10681 cgatcccgca gattgcgcaa gcgcttcaag aaggccggcg cgaagagttg acgctcacgg
    10741 taccggaggg caagcggctc gaagaagtgg cgcaaatcgt cgggtcgcaa attccgcaga
    10801 tcgacgcgaa cgaattctcg ctgctggcga gcgacgcttc gctgtggaag tcgcagttct
    10861 cgttcctgtc ggacatcccc gaaggcggat cgctggaagg ctatctgttt cccgacacgt
    10921 accgcctgcc gctcgaaacc gacgcgcgcg atgtgatgac tcgcatgctg cgcaacttcg
    10981 acgcgcaggt cacggcgcaa atgcgcgccg acgcgcaggc gtccggccgc agcctgtggg
    11041 acgtaatccg attggcgtca atcgtcgagc gcgaagcggt cgtcgcggaa gagcgcccat
    11101 tgatcgccgg cgtgtacctg aaccggctgg cgcagggcat ggcgctcgac gccgatccca
    11161 caatccagta cgcgttaggg caaagccgcg agccgggcaa ctggtggccg caattgacgc
    11221 tggaagatta tcagggtgtg gccgcgccgt acaacacgta cctcaatccc ggcctgcccc
    11281 ccggcccaat cgccagcgcg ggccgaggat cgatcgaagc agtgatctat cccgagcaga
    11341 cggatttctt cttcttccgg gcgtcgtgca gccgagacgg cactcaccag ttcgcccgca
    11401 cgctcgaaga gcacgccgcg aacgagtgcc cttgagagtt agaggttgga gtcagagttc
    11461 gagttccttc agttccgtga gtgcgttgcg agggaactgg cggaactcag ggaactgact
    11521 tgagactccg ctcccgctgg gtcacagacc cagcggcttg agcaacggac gtgggtcaca
    11581 gacccccttt gagcgtgaaa ttatccgtct cgcttttcgc tttcctcacc tttgcgctgg
    11641 cggcctgctc cactggttct accgcgccgg tcgtcaagat cggtctggct gcaccgttcg
    11701 agggccgcta ccgcgccatc ggctacgagg ccatttacgc cgcacgcctg gcgatccgcg
    11761 agatcaacgc tgccggtgga atcgacggct accgcgtcga gttgtccgcg ctcgacgacg
    11821 gcggcgatcc ggcaatggcc atcgagcagg cgcgaaagat ggcgatcgat ccgcaggtga
    11881 tcgccgtcat cggccattac cggccggaga caaccggcgc ggcgaccggg cattactgcg
    11941 cggagcgatt acctctcctc gccgtcgagc ctgttcgaac gagcgtctgc cacgaggtgc
    12001 tcgcgctcac gccgccgatc acgacgaccc tctcgtcttc cgatcccgca tcattcctct
    12061 ttgccgggac ggtcccgcat cccgcagatg tgcccgaagc ggccgcgttc gtcgaggctt
    12121 acaatgccat cccgatcgac gggacgcggg ccgggccgat cgcgctccag acatacgatg
    12181 cgatgtatct gctattcgat gcgctgagga ggaacttaca ggctgagggc aggccgtcgc
    12241 gggatgggat ggcgcgcgcg ctggcagagt cagacttcac aggtctgggt ggggtgtatc
    12301 ggttcgacgc ggaaggcaat cgaacccaga tgaagacgta tacgtatcgt tatagcaaag
    12361 atcgtgagcc agagttgatc aggtgattca agaactcggg gcaatcccct gctcaattgc
    12421 tcaattgctc acttcttcac tttatccttg tcgcgcggcg gcagtttcgc cagcatacgg
    12481 cgtgaggtga tcacgtacca ggcaatcgcg gcaaacagcg ctaccgctcc agccacgaca
    12541 taggggttca tgttggtctc cgatctgtct attcaatcac accgtaaaac agcggcagcg
    12601 gctcaacccg tgcgtcgctg aaagtgacgg cgccaagcaa tcgcgtctcg gcctccaccc
    12661 gcctgtgttc gtcattggcg tggacggtga acagtcgcgc gcccggatcg attcgatctc
    12721 cgaccttcac gtgaacctcg acgccaacgg cgtggtcgat cggatcgcct ttcttctcgc
    12781 gcccggcgcc cagcatgact gccgacaggc cgatttcgag cgcgtcgacc cgcgcgacgt
    12841 acccgcctcg cggcgacggc acgtcgcaga cgatcgtcgc gcgcggcaaa tggtcaggct
    12901 gctccacgac cgacgcatcg ccgccctgca ccgttaccag ctcgacgaac tttcgccagg
    12961 cccgcccctc gtgaatgagc gacccggccc gcgcgcggcc ttcttcaacc gacgctgctt
    13021 tcgcgccgag gcgcagcatg tgcgcggcga cggtcaggca atgctcggcg aaatcgctgg
    13081 ggccgcgacc acgcagcgtc ttgatagcct cgcgcagctc gagcgcattc cctaccgcgc
    13141 tgcccagcgg ctggttcatg tcgctgatca gcgcgacggt gcgccgcccc aacatcttgc
    13201 cgatgtcgac catgatgccc gcaaggacgc gcgcgtcggc ctcggtcttc atgaacgcgc
    13261 cggacccaca cttcacgtcc agcacgatac cgtccgcgcc gcctgcgatc ttcttgctca
    13321 tgatgctcgc ggcgatgagc ggcagcgacg ggacagtgcc cgtcacgtcg cgaagcgcat
    13381 agagcttgcc gtcggcgggc gccagatcga ccgactggcc ggtgagcacg atgccggcgc
    13441 gcttgagctg cgcgcgaaac tcgtcttcgc tcagattcgt gcgaaagccc gggatcgatt
    13501 ccagcttgtc gagcgtgccg ccggagaacg acaggccgcg cccgctcatc ttgccgaccg
    13561 gcagcccact cgacgcgacc aggggcgcga cgaccaggct taccttgtcg ccgacgccgc
    13621 cgctcgaatg cttgtcgacg acgaacggcg cgatgtcgtg caggtcgagc gtcttgccgg
    13681 agcgcgccat cgccagcgtc aggtcggtcg tctcgcgggc ggtcatcccg cgcagcagca
    13741 ccgccatgca ccaggctgct gcctggtagt cggggatgtc gccgcgcgtg taaccctgca
    13801 cgaagaagtc gatctcctcg cgggtcagct catggccgtc acgtttcttt tcaatgacgt
    13861 cgaccgcgcg catgctcccc ccagggttac tggatccgag tgacccatta ggctgcgaat
    13921 cgagctcggc gagccgcccg gcggcatcgg cgagcgggac gtgaaagcgc agccgcacgc
    13981 tggcgggcgt gcgctcgctc gccggcagcg cgccgatcca ctccttcggc atcagaagag
    14041 cgtgggcgaa gtagcgcgcc tcgtctgcgg tgaaaccggc attgccggct atgccccgtt
    14101 cgcgcgccca gcgcgtgccg ctcacgtaac gcgcaaccac acgggcgatg gtcgggcgca
    14161 cactgtagcg atcggtcgcc tcgaaagatt gatgtgacag gtcgaccaaa tcgggctgcc
    14221 acatctcggc gaccgggttg cgcaagatct cctcgatcgg cacgggcggt cgctcgacgt
    14281 tgtgcagcgt caagaacacg atcgctgctt cgtcaagctc ggcgcggcga tcgtcggata
    14341 gcggatgcgg ctcgttcata tgggcgctct gttgcgtgcc cgtagattat accctcgttg
    14401 acgagaacgg gaaatggcgg gagaatccga gcgcgtttga t
//
LOCUS       DBNHXB010000163         4714 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2386219, whole genome shotgun sequence.
ACCESSION   DBNHXB010000163 DBNHXB010000000
VERSION     DBNHXB010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2386219"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..768)
                     /locus_tag="AC162G_06375"
     CDS             complement(211..768)
                     /locus_tag="AC162G_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075471.1"
                     /translation="MAASITPRETGVQETGVRLSYLDEVAETMRDDAIAQTIAKLNSR
                     AHHLDLANLLGRPDFLDYFKHGLASGAANVLAASDQNVQAVYVYDPALDAGSEAGEDP
                     PRDTGVHLLVRVTTSSAALMALIASLDRALAASLKDLPAPGFRQRESVLDVNLLSDDD
                     MRRGVNYAGGLSGVFAPPLRIWQRA"
     gene            complement(897..1412)
                     /locus_tag="AC162G_06380"
     CDS             complement(897..1412)
                     /locus_tag="AC162G_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075472.1"
                     /translation="MANAQMVQTQADMMLENALAFCADKTFQGDTRQARLALTSGRCD
                     VCDYLCYSLAKQVGEYLGQMDAAVKAVYMFQPEYARSSEATAACRSAGINMIAWVERK
                     SAALSALTEALDAALSERRRRLDCVNATAACYTLNVHLVDDADVRERRGYGALIGSLF
                     VRPIRVWAHAN"
     gene            complement(1452..3077)
                     /locus_tag="AC162G_06385"
     CDS             complement(1452..3077)
                     /locus_tag="AC162G_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR5075473.1"
                     /translation="MSAIGAFCVSVHKDVCINARMPGKCAVCAEVCPHGVFALDAVTG
                     EMHIRNYNACVGCRICAEFCPADAIRINPAESERISRYPWTLGTIEEIHYKAQTGEYL
                     LRGFGTLGPTPHFDTITVVPSQIASPAPLDKYREECDMEVVIGEDTCEKPIRLKAPFV
                     WAAMSFGALSKEAKMALAIGAAMTGIATNTGEGGLVPEEAWLSHGYADKERKRKKWDP
                     GGYLIIQWSTGRWGVSVDYLNSGDAVEVKIGQGAKPGMGGHLLGAKVTEDVSRVRGVP
                     VGSDALSPCRYYDVQTLEDMKHMVGFLRDVTDYQVPILFKLGPSRPYADVAACIEAGA
                     DAISIDGLIGGTGCSPDIVTQGVGIPTIACIPPAVQALRDYGVHRKVKLIALGGIRNG
                     LDAFKAIAMGADAVGFGSAAEVAMGCRVCFACQKGNCPYGITSQKPEMRARLDPIETG
                     RRLANFIHACTEEVKIVTMLSGHRRLAELSPEDLRAMDLNTAAITGLKLIGTDRPLAM
                     WEQGYQPALGVLSSGNGHGNQRRQRVTKRKQRA"
     gene            complement(3161..3811)
                     /locus_tag="AC162G_06390"
     CDS             complement(3161..3811)
                     /locus_tag="AC162G_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GltB/FmdC/FwdC-like GXGXG domain-containing
                     protein"
                     /protein_id="HIR5075474.1"
                     /translation="MKTIDARSKDLMSVNRTLKQSLDDQPMAVKNAGHLHGLGAGLRH
                     GQLMVRGNAGDYLGVLNAGATITVTQDAGKYVADNMTGGTVIVEGSAEYGAAQYCYGG
                     TVIVRGNAGDFTATMNKGATIIVCGDVGDEAGTYMLRGDLIVIGNAGANFANYLIRGD
                     VYVGGEWQSLGHNTRVEPLSDEDVAKLRAYFETYDIDADPAHFKKIVAASEKPFYN"
     gene            complement(3826..>4714)
                     /locus_tag="AC162G_06395"
     CDS             complement(3826..>4714)
                     /locus_tag="AC162G_06395"
                     /inference="COORDINATES: protein motif:HMM:NF016328.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HIR5075475.1"
                     /translation="SGAPNDVIRSILAAGRSAGLLDGVVMLDLDPRELKPVARVANTV
                     EDIAGSVGPQYLWAPVFDALNEAVFERRMENVAVVGTPCAAQAVRKIKASPQTRLRPY
                     QDAIRLSIAMFCTGIYKSEVIDDILVKRMGVPRNQVKRLEVPADRESLRAVLWDGSVR
                     TIPHQVVEHYTRAGCATCDDYLGESADLAIGKLGAGEDASTLIVRTQTGDLFARNAIQ
                     MNLLEATGDVDMTALAAAGEKGRRERAQAFKDLHILMLDALADPLKRGEAITHFVRLY
                     RTPTRSEAPVASPRGCSAC"
BASE COUNT          807 a         1559 c         1459 g          889 t
ORIGIN      
        1 agacgaggtt gcttgctcac ccctcgcgaa gatcgctcgg agacgtacct cctttcctcg
       61 ggataaatca agacgccagc ctcattctag atgagaagta tacaccgaac agtgacaaat
      121 agcatactgc gaaagtgata gatggcgtaa gccccaaaaa gcaagaggcg gaagggaacc
      181 acccctccgc ctccggctca aggagaagcg tcacgcgcgc tgccaaatcc taagcggcgg
      241 cgcgaatacg cccgatagtc cccccgcata gttcacacct cgccgcatat cgtcgtcgct
      301 gagcaagttt acgtcgagga ccgactcgcg ctgccggaag ccgggtgcgg gcagatcctt
      361 caggctcgcc gccagcgccc gatccagtga agcgatgagg gccatcagcg cagccgacga
      421 cgtggtcacc cgtaccagca ggtgcacacc cgtgtcgcgc ggcgggtctt cgccggcctc
      481 gctgcccgcg tcgagcgcgg gatcataaac gtacaccgcc tgcacgttct ggtcgctcgc
      541 ggccaacacg tttgccgcgc cggacgccag cccgtgcttg aagtaatcga ggaaatccgg
      601 acggccgagc aggttggcca gatcgagatg gtgtgctcga ctgttgagtt tcgcgatagt
      661 ctgggcgatc gcgtcgtccc gcatcgtctc ggcgacttca tcgagatacg atagccgaac
      721 gccggtttcc tgcacgcctg tttcacgggg agtgatgctc gcagccatac acagcctcct
      781 tcgagacgac tatatcagct tgcccatgaa aagcacatag aaaacggcac ccaagacctg
      841 aaaaggacgt gagaggccgg tcggatgagc gtcgagggtg gcggcatgga gcaggctcag
      901 ttggcgtgcg cccacacccg gatcgggcgc acgaacaggc tgccgatcag cgcgccgtag
      961 ccgcgccgct cacgcacgtc ggcatcgtcc accaggtgaa cgttgagcgt ataacacgct
     1021 gctgtggcat tgacgcagtc cagcctgcgc cgcctctcgg acagggcggc atccagagcc
     1081 tcggtcagcg cactgagtgc ggcgctcttt cgctcgaccc aggcgatcat gttgatgccc
     1141 gccgaccgac aggcggccgt cgcttcgctg gaccgggcgt actctggctg gaacatgtac
     1201 acggccttga ccgccgcgtc catctggccc aggtattcgc cgacctgctt cgccaggctg
     1261 tagcatagat aatcgcacac gtcgcaccgg ccgcttgtca gggccagtcg cgcttgacgc
     1321 gtgtcaccct ggaacgtctt gtcggcacag aaggcgaggg cattctccaa catcatatcg
     1381 gcctgagtct gcaccatctg cgcattagcc atctcacacc tcccgctgat gatccgcagt
     1441 cgaaattcct tttacgctcg ctgctttcgt ttcgtcaccc gctgccggcg ctggttccca
     1501 tgaccgttac cgctggacaa tacgccgagc gccggctgat agccctgctc ccacatcgcc
     1561 agcggccggt cggtgccgat cagcttcagc ccggtgatgg ctgccgtgtt cagatccatc
     1621 gcacgcaggt cctcgggcga cagctcggcg aggcgacgat ggccggacag catggtgaca
     1681 atcttgactt cttcagtgca ggcgtgtatg aaattcgcca gccgccgccc ggtctcgatc
     1741 gggtcgagcc gcgcgcgcat ctcgggtttc tggctggtga tgccgtacgg gcagttgcct
     1801 ttctggcagg cgaagcagac gcggcagccc atcgccacct cggccgcgct gccgaagccg
     1861 acagcatccg cgcccatggc gatggccttg aaggcgtcca gcccgttgcg aatgccgccg
     1921 agcgcgatca acttgacttt gcggtgcacg ccgtagtccc tcagtgcctg cacggcgggc
     1981 gggatgcagg cgatggtggg gatgcccacg ccctgcgtga cgatgtcggg cgagcaaccc
     2041 gtcccgccga tcaggccgtc gatgctgatc gcgtcggcgc cggcttcgat gcaggcggcg
     2101 acatcggcat acgggcgcga aggccccagc ttgaacagga tcggaacctg ataatcggtc
     2161 acgtcacgca ggaagccgac catgtgcttc atgtcttcga gtgtctggac gtcgtagtat
     2221 cggcacgggc tgagcgcgtc ggaaccgacc ggcacgccgc gcacgcgcga cacgtcctcg
     2281 gtcaccttcg cgccgagcag gtggccaccc atgccgggct tggcgccctg gccgatcttg
     2341 acctcgaccg cgtcgccgct gttaaggtaa tcgaccgaca cgccccaacg cccggtagac
     2401 cactggatga tcaggtatcc gccgggatcc cacttcttac gcttccgctc cttatcggca
     2461 tatccgtgcg acagccaggc ttcttcgggg accagaccac cctcaccggt attggtggcg
     2521 atgcctgtca tcgccgcgcc aatggccagc gccatcttgg cttccttgct cagcgcgccg
     2581 aacgacatgg ccgcccacac gaaaggcgct ttcaagcgaa tcggtttctc gcacgtgtct
     2641 tcgccgatga cgacttccat gtcgcactct tcgcgatact tgtcgagcgg cgccgggctg
     2701 gcgatctggc tgggcaccac cgtgatcgtg tcgaaatgcg gcgtcggccc aagcgtgccg
     2761 aacccgcgca gcaggtactc gccggtctgc gccttgtagt ggatctcttc gattgtcccc
     2821 agcgtccacg gatagcgact gatgcgctcc gattcggccg ggttgatgcg gatagcgtcg
     2881 gctggacaga actcggcgca aatgcggcag ccgacgcagg cgttgtagtt ccggatgtgc
     2941 atctcgcccg tgaccgcatc gagcgcgaat acgccgtgcg ggcagacctc ggcgcacacc
     3001 gcgcacttgc ccggcatgcg cgcattgatg cagacatcct tgtgtacgct gacacaaaag
     3061 gcgcctatcg cactcatcgt cgtctcctct ctctggaggt tggagattgg agataggaag
     3121 gcgtgcctct aacttccaac ctccaatctc caacttccac tcagttatag aatggttttt
     3181 ccgacgcggc gacgatcttt ttgaaatggg ccggatcggc gtcgatgtcg tacgtctcga
     3241 aatacgcacg caacttggct acgtcctcgt cgctcagtgg ctcgacgcgc gtgttgtgcc
     3301 ccaagctctg ccactcgccg ccgacgtaca cgtcaccgcg aatcaggtaa ttggcgaagt
     3361 tggcgccggc gtttccgatg acgatcaagt cgcctcgcag catgtaggtg ccggcttcat
     3421 cgccgacgtc tccgcacacg atgatcgtcg cgcccttgtt catcgtcgcg gtgaaatcgc
     3481 ccgcgttgcc gcgcacgatg accgtgccgc cgtagcagta ttgcgccgcg ccgtactctg
     3541 cgctgccctc gacgatgacc gtgccgccgg tcatgttgtc ggcgacatat ttgccggcgt
     3601 cctgcgtcac cgtgatcgtc gcgccagcgt tcagcacgcc caggtagtcg ccggcattgc
     3661 cccgcaccat cagttggccg tgtcgcaggc cggcgcccag cccgtgcagg tggccggcgt
     3721 tcttgaccgc catgggctga tcgtcgaggg attgcttcag cgtgcggttg acgctcatga
     3781 gatctttgct tcttgcatcg atggtcttca tcgctcgcct cgcttctagc aggcactgca
     3841 gccgcgcggg ctggccacag gggcttcaga gcgcgtcggg gtgcggtaca ggcgcacgaa
     3901 gtgcgtgatg gcctcgccgc gcttgagcgg gtcggccagc gcgtcgagca tcaagatgtg
     3961 caggtctttg aacgcctggg cgcgctcgcg cctgcccttt tcaccggcgg cggccagcgc
     4021 agtcatatcg acgtcgcccg tcgcttccag caagttcatc tggatcgcgt tgcgggcgaa
     4081 caggtcgcct gtctgtgtgc gcacgatcag cgtggacgcg tcctcgcccg cgcccagctt
     4141 gccgatggcc aggtcggccg actcgcccag gtagtcgtcg caggtggcgc agccggcgcg
     4201 cgtatagtgt tccacgacct gatgcgggat ggtgcgcacg ctgccatccc agagcaccgc
     4261 gcgcagcgat tcgcggtcgg ccgggacctc cagccgcttg acctggttgc gcggcacgcc
     4321 catgcgcttg accaggatgt cgtcaatgac ttctgacttg tagatgccgg tgcagaacat
     4381 ggcgatgctc aggcggatgg catcctggta cggccgcaag cgtgtctggg gtgatgcctt
     4441 gatcttgcgc acggcttgcg cggcgcacgg cgtgccgacg acggcgacgt tctccatgcg
     4501 ccgttcgaag accgcctcgt tgagcgcatc gaagacgggc gcccacaggt actgaggccc
     4561 gacgctgccg gcgatgtcct cgaccgtatt cgccacgcgg gcgacgggct tgagttcccg
     4621 gggatcaaga tcgagcatca ccacgccgtc cagcaatccg gcgctgcgcc cagccgccag
     4681 gatgctgcgg atcacgtcgt taggtgcgcc ggac
//
LOCUS       DBNHXB010000164         9725 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3728396, whole genome shotgun sequence.
ACCESSION   DBNHXB010000164 DBNHXB010000000
VERSION     DBNHXB010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9725)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9725)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9725
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3728396"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="AC162G_06400"
     CDS             <1..604
                     /locus_tag="AC162G_06400"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5075476.1"
                     /translation="EQIPSSQGVGQRQMKLTIRPLTPDLWPALEDLFGASGACNGCWC
                     MHWRIGSAYHKRSRAKNKAAFRDIVRRGPPPGLLAFDGDVAVGWCQLTPRDSLPWLEH
                     DWRVKRVDDVPVWCLSCFYVRKGYRRQGVTSALIEAALKAAKRANAPALEAYPRDTDE
                     SPGSTFTGYASTFARAGFKTVARHTPSRPIMRYDLKAIRR"
     gene            872..1252
                     /locus_tag="AC162G_06405"
     CDS             872..1252
                     /locus_tag="AC162G_06405"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5075477.1"
                     /translation="MEAEVEAAVQAGEQFWAAHNAEGATVGWLWVKCSLEGLPPSAAF
                     LYQILVKPEVRRQGYGTAMLAALEDVLAADGWSELRLNVWHTNEAGRRLYERAGYKLV
                     ERFSTKRQLHKRLPPADPNRPHAG"
     gene            complement(1297..2355)
                     /locus_tag="AC162G_06410"
     CDS             complement(1297..2355)
                     /locus_tag="AC162G_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005479769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5075478.1"
                     /translation="MNKRILIPLISALALVMSACAAQPATPTGPRTLTVLTHESFVLS
                     DDVLEEFESANNATVQFLKQPDAGQALNRAILTKDNPEADVLFGVDNTFMSRALDEGI
                     LEPYRSSALAGIPAEFQLDPENRLTPIDFGDVCLNYDKAYFAERNLQPPQALADLARP
                     EYKDLLVVENPASSSPGLAFMLATVARFTEESWLDYWADLENNGVKVVEDWTTAYYTE
                     FSGSSGKGPRPIVVSYASSPPAEVFFAESPLEEAPTAAVTAPQTCFRQIEFAGILKGA
                     KNRDLAEKWIDFMLSKRYQEDVPLNQFVYPVNPQAALPEVFVKHSQIAQEPANLPPEA
                     IAANRDRWIEEWTAMVLR"
     gene            complement(2432..3076)
                     /locus_tag="AC162G_06415"
     CDS             complement(2432..3076)
                     /locus_tag="AC162G_06415"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIR5075479.1"
                     /translation="MRALLFANGYLVSRDVVARWMQPGAIVIAADGGAQNALDAGVTP
                     DTVIGDLDSLPDSVRADLNRMGVRFLVNSARKDETDLELAIGHALERGATEIVILSGL
                     GNRWDQSLANMLLLTLPALRDAPARIEDERHSIRAISSGMQARVEGAIGDTLSLIALG
                     GDARGVTIEGCEYPLRDAVLPFGKTLGISNVLSEPAARVSVQEGVVLVIRIRKT"
     gene            3424..3987
                     /locus_tag="AC162G_06420"
     CDS             3424..3987
                     /locus_tag="AC162G_06420"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR5075480.1"
                     /translation="MPRLTRIYTRKGDRGTTSLVGGRRVAKDSLRVNAYGSVDELNSQ
                     IGLAISLGLHEDLVRILRTVQNDLFHLGADLATQEEDKQRVQVPQIEPRHVDALEQVI
                     GRFAAELGPLENFILPGGSPGAAALHVARTACRRAERECIALSRAEPIGAHVLAYLNR
                     LSDALFMMARYENHARGVSDAYWDSRA"
     gene            4203..5744
                     /gene="dnaX"
                     /locus_tag="AC162G_06425"
     CDS             4203..5744
                     /gene="dnaX"
                     /locus_tag="AC162G_06425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR5075481.1"
                     /translation="MAQALYRKWRPQTFDDVVGQEHIVQTMHNAVASGRIAHAYLFSG
                     PRGTGKTTMARLLAKAVNCLNPDPAQRPDDTCAICAAIAEGRLLDLIELDAASNRGID
                     EIRDLRDKINFAPNEARYKVYVIDEAHMLTEPAFNALLKTLEEPPPHAIFVLATTDPQ
                     KVPVTIVSRCQPFAFRRLTLDEIVSRLRQLVDAEGLHADPVALQIVARQATGSMRDAI
                     SLLDQLAAGSEAITVERAQDVLGAGALEHVMALADALASGDAAGGLSAINTAIDRGAD
                     ARQLARQIVEYLRGLMLIAFGDTSQVNVASEQRRHMEEQATRLEADRLIRATKLFNQA
                     ASELKPGWQPQLPLELALVDALNRPAQPAQAAPAPAQTTRAPAPSAPARSQAATASVA
                     PGSPDPIESTPPEAPATGLAFDIVSDQWPALLAELRSRSVQTWGLMNSCQLIGVEGDT
                     IVLQWPSAMLRDKFEKGKDKRLVEDVLSEMIGRRVRTRCIVDDDPVVQEARRLGAQVR
                     LVSDQ"
     gene            5850..6197
                     /locus_tag="AC162G_06430"
     CDS             5850..6197
                     /locus_tag="AC162G_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR5075482.1"
                     /translation="MSKKGGFRMPQGGPNPANLMSQLQKLQDEMAKTQDALADETIEV
                     TAGGGAITIVITGQQRLRSIKLSPEVVDPSDVEMLQDLIVAAVNEAIERSQMYAAEKV
                     KGLTGGLGLPPLR"
     gene            6262..6861
                     /gene="recR"
                     /locus_tag="AC162G_06435"
     CDS             6262..6861
                     /gene="recR"
                     /locus_tag="AC162G_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR5075483.1"
                     /translation="MDTTPKAVTRLIEAFAQLPGIGPKSASRLTYFLIRDASGVVESL
                     AEALRNLKTSTVFCSTCFNIADTSPCAVCASEERDGTVICVVEEPLDVQAIERTREYK
                     GRYHVLHGAISPIDGVGPDDLRIRELLNRVATGAVREVILATNPSLEGENTAMYIQKK
                     LAGMGVKVTRLARGLPVGGDLEYADEITLARALAGRSEM"
     gene            6870..7436
                     /locus_tag="AC162G_06440"
     CDS             6870..7436
                     /locus_tag="AC162G_06440"
                     /inference="COORDINATES: protein motif:HMM:NF019632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HIR5075484.1"
                     /translation="MDDTSVFDNLSAEPRDMVGLFDRIKRARSSLKQTYAHLSDAQMV
                     APGADGGWSVKDHLAHLTTWEAGIAALLRREPRWAAMGLDDETVRQNDEAGVNAVIDR
                     HNKDRARADVLADFEQTHRDLLAVLGQLTFDDLLKTYSYYQPNEPGVGSGRPILAWIA
                     GNTYEHYAEHENWIRAVAEYVTGGGRAG"
     gene            7433..7990
                     /locus_tag="AC162G_06445"
     CDS             7433..7990
                     /locus_tag="AC162G_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075485.1"
                     /translation="MKKREANKIAGISDEAVKARTGRTWAEWIRVLDKAKAYEMPHKD
                     IAIYLSEKHQVPDWWTQMVTVGYEQARGLREKHERPEGYQVSASKTLDVPVGTLYAAW
                     ADEAMRARWLKRKSLAIRGATPPKTIHITWTGKSSVEARFYARGQARSQVTVQHSKLV
                     SAEDGEKSKAYWAQALDRLKRVLEG"
     gene            8155..8508
                     /locus_tag="AC162G_06450"
     CDS             8155..8508
                     /locus_tag="AC162G_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR5075486.1"
                     /translation="MTGFSDSLRGYAYDIHKRDNFTCRYCGADGTKSFDTWLTLTVDH
                     LVPKGDPNRDNPDYMVTACGFCNTADNRYFDLVEQRGLKLDGLTPDELVAQRLPYVQR
                     TRQSYRSFWETNVRK"
     gene            8612..8998
                     /locus_tag="AC162G_06455"
     CDS             8612..8998
                     /locus_tag="AC162G_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR5075487.1"
                     /translation="MSTGKSTDNQPPPDEMDKFIRAYLRGAELRGAFLSGADLFDADL
                     AGADLRGADLGGALLSDANLCRADLSGANLVGADLSEANLCGADLRDADLNGADLNGA
                     RYDRRTVWPEDFDPQSAGAVEVADAP"
     gene            8988..9383
                     /locus_tag="AC162G_06460"
     CDS             8988..9383
                     /locus_tag="AC162G_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HIR5075488.1"
                     /translation="MRHRINIQATTSITYLAWGHIEVTVDGHAHKFKDCKVWPGGAKA
                     WDWTMTGTRHRPGIQPADIEEIIDQGVEVMILSRGMQLMLHTCPETEQLLRSRGIEYH
                     IEETGRAVELFNDLMKRGQKVGGVFHSTC"
     gene            9443..>9725
                     /locus_tag="AC162G_06465"
     CDS             9443..>9725
                     /locus_tag="AC162G_06465"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR5075489.1"
                     /translation="MNFFDPKPAAERYARGRPFFHPLVIRRIKSYLSLIEPVSRAIDV
                     GCGTGLSTIALKEIARHIVGVDISSEMIALAPQDSEGYFMRRAVLLLLVI"
BASE COUNT         1880 a         2966 c         3127 g         1752 t
ORIGIN      
        1 ggaacagatc ccgtcttctc aaggggtggg gcaacgccag atgaaactta cgatccgccc
       61 gctcacgccc gatctatggc ccgccctcga agacttgttt ggcgcaagcg gcgcctgtaa
      121 tggctgctgg tgcatgcact ggcggatagg cagcgcctac cacaagaggt cccgcgccaa
      181 gaacaaggcg gcatttcgag acatcgtcag gcgtggtccg cccccaggct tgctcgcctt
      241 cgacggcgac gtcgccgtgg gttggtgcca actgacgccg cgagattcat tgccctggct
      301 cgagcacgac tggcgcgtca agcgcgtcga cgacgtccca gtctggtgcc tgtcctgctt
      361 ctatgtgcgc aagggatatc gcaggcaggg agttacgtct gccctcatcg aggcggcctt
      421 gaaggctgct aagcgtgcga atgctcctgc gctcgaagcc taccctcgcg atacggacga
      481 gtcgccaggc tccacattca ccggatatgc ctccaccttc gcacgtgccg gcttcaagac
      541 cgtggcgcgg catacgccgt ctcgcccgat tatgcgctac gatctgaagg cgatcaggcg
      601 atgacgtttg ggtggccgtt cgcaatgaat gtgtcgaagg ccgtcgtcac aaccgatcaa
      661 gtttgacttt ggtggacact gtctgtcggg tcacgttctg gccgttagtg ggctttgtgc
      721 aatagaagct gacgcggcgc agcttgataa agcctatggc gatgctggtt gtcttggctc
      781 ccctcacgct cgacgagcgg cccgtcttca cctcagcgat cgtcgcggac tacgtagatt
      841 cccttgtggg tcgaggtgaa gacgggccgg gatcgaggca gaggtcgagg ctgccgttca
      901 ggccggggag cagttctggg ccgcgcacaa tgccgaaggc gccacggtcg ggtggttgtg
      961 ggttaagtgc tccctggaag ggctgccgcc ctctgcggcc ttcctatacc agatccttgt
     1021 caagccggag gtccggcgcc aaggctacgg caccgccatg ctggccgcat tggaggacgt
     1081 tctggctgcg gacggctgga gtgagctgcg cctcaacgtc tggcacacca atgaggcggg
     1141 caggcgcctg tacgagcggg ctgggtacaa actggtcgag cggttctcga ccaagcgcca
     1201 gctacacaag cgactgccac ccgccgaccc caaccgacct catgccggct gaggagaggg
     1261 cgtcattgcc aagcagtcgc cctctcacgg ttggccttat cttaatacca tcgccgtcca
     1321 ctcctcaatc caccggtcgc gattcgcggc aatggcctcc ggcgggagat tcgccggctc
     1381 ctgcgcgatc tgcgaatgct tgacgaagac ctcgggcagc gcggcctgcg ggttcaccgg
     1441 gtacacgaac tggttgagcg gcacgtcctc ctggtagcgc ttgcttagca tgaagtcgat
     1501 ccacttctcg gccaggtcgc ggttcttcgc gcctttcagg atgccggcga attcgatctg
     1561 ccggaagcac gtctgcggcg cggtgacggc cgccgtcggc gcttcttcga gcggcgactc
     1621 ggcgaagaac acctcggccg gcgggctgga ggcgtagctc acgacgatcg gcctggggcc
     1681 tttgccgctc gacccgctga actcggtgta gtacgccgtc gtccagtctt cgacgacttt
     1741 cacgccattg ttctccagat cggcccagta gtcgagccag ctttcttcgg tgaatcgggc
     1801 gacggtcgcc agcatgaacg ccaaccccgg cgacgagctg gcaggattct cgacgaccag
     1861 caggtctttg tattcgggcc tggcgaggtc ggccagcgcc tgcggcggct ggagattgcg
     1921 ctccgcgaag tacgccttgt cgtagttcag gcatacgtcg ccgaagtcga tcggcgtcag
     1981 acgattctcg ggatcgagct gaaactcggc aggtatcccc gccagcgcgg acgaccgata
     2041 cggttcgagg atgccctcgt ccagcgcgcg gctcatgaac gtgttgtcca cgccgaacag
     2101 cacgtcggcc tctgggttat ccttcgtcag gatggcgcgg ttaagcgcct gcccggcgtc
     2161 gggttgcttg aggaattgca cggttgcgtt gttggccgac tcgaactcct cgagcacgtc
     2221 gtcgctgagc acgaacgact cgtgcgtcag cacggtcagc gtgcgcgggc cggtgggggt
     2281 tgccggctgc gcggcgcacg ccgacatcac gagtgcaagc gcgctgatca gtgggatgag
     2341 aattcgctta ttcatgtgaa acacctccta ttgcgtattg catgtggcgt ggtgcgtgtg
     2401 atgtaacaaa ctacggaaca cgcaccacgc atcacgtttt gcggatacgg attaccagca
     2461 cgacgccttc ttgcacgctg acgcgcgcgg ccggttcgct caacacgttg ctgatgccga
     2521 gcgtcttgcc gaacggcagc acggcatcgc gcaggggata ctcgcagccc tcgatggtga
     2581 cgcctcgcgc gtcaccgccc agcgcgatga gcgagagggt gtcgccgata gcgccttcga
     2641 cgcgcgcctg catgccgctc gagatcgcgc ggatcgaatg ccgctcgtct tcgatgcgcg
     2701 ccggcgcgtc gcgcagcgcg ggcagcgtca ggagcagcat gttcgccagc gactgatccc
     2761 agcgattgcc cagcccgctc aggatcacga tctccgtcgc gccgcgctcc agcgcgtggc
     2821 cgatcgccag ctccagatcg gtctcgtcct tgcgcgcaga attgaccaga aagcggacgc
     2881 ccatgcggtt gaggtcggcg cggacggagt cgggtaggga atcgagatcg ccgatgaccg
     2941 tgtcgggggt gacgccggcg tcaagcgcat tctgcgcgcc gccgtcggct gcgatcacga
     3001 tcgcgccggg ctgcatccag cgcgcgacga cgtcgcgcga taccagatag ccgttggcga
     3061 ataacagtgc gcgcatgttg tctccagaaa acaaagacca cccggctgcc gggtgatctg
     3121 cttctgtctc tttccctacg ccggcattac ccgtcaggtt cggaccgtct gcgccatagc
     3181 cacgctgagc aagcgcactc tcagccgggt ctaacccggc tccggttgga ctattcaatt
     3241 cacgataact atcgtgataa taattatagc accgtttttc tcgcgtgtga cgggcgagtc
     3301 tcaggcgtcc tctgcggtga gacagaatcg atcgcgcgag acgactgcat agcgggtggc
     3361 ccatagaacg ggtataatcc tgtgcggcgc gcgaagcacg gagcatcgaa atagaggata
     3421 cgtatgcctc gtctgacacg aatctacacc cgcaagggtg atcgcggcac gaccagtctc
     3481 gtcggcgggc ggcgcgtggc gaaggattcg ctgcgcgtca acgcgtacgg ctcagtcgac
     3541 gagctgaatt cgcagatcgg cctggccatc agcctgggcc tgcacgagga tctcgtgcgc
     3601 atcttgagga cggtccagaa cgatctgttc cacctgggcg cagaccttgc gacgcaggag
     3661 gaagacaaac aacgggttca ggttccgcag atcgaaccgc gccatgtcga cgcgctcgaa
     3721 caggtgatcg gccgattcgc agcggaactt ggcccgctcg agaatttcat cctgcccggc
     3781 ggctcgccgg gcgcggcggc gctgcacgtg gcgcgcaccg cctgccgccg cgccgaacga
     3841 gagtgcatcg cgctcagccg cgccgagccg atcggcgccc acgtcctggc gtatctcaac
     3901 cggctgtccg atgcgctgtt catgatggcg cgctatgaga atcacgcgcg cggcgtgagc
     3961 gacgcctact gggacagccg cgcgtgacgc gcgtatcaca tatcgcgtat cgctcatcgc
     4021 gtatcgctta tcgcgcatca cttgtcgtgc agcgtcgatt gtctccggcg atttgcgatc
     4081 agtggtcgct atcggcagtt ggttatcggc aatacgccat cagcgatagg cgataagcaa
     4141 taggctctta cttgccgcag cctgtcgacc atgctacact tgtcgcacgc gaaggagatc
     4201 gaatggcgca ggcattgtat cgcaagtggc gaccccagac gttcgacgac gtcgtcggcc
     4261 aggagcacat tgtgcagacg atgcacaacg cggtcgcgtc cggccgcatc gcgcacgcgt
     4321 atctcttctc cggcccgcgc ggcacgggca agacgacgat ggcgcgcctg ctggcgaaag
     4381 cggtcaactg cctcaacccc gatcccgcgc agcgccccga cgacacgtgc gccatctgcg
     4441 cggcgatcgc cgagggccgc ctgctcgacc tgatcgagct ggacgccgcg tcgaatcgcg
     4501 gtatcgacga gattcgcgac ctgcgcgaca agatcaactt cgccccgaac gaggcgcgtt
     4561 acaaagtcta cgtgatcgac gaagctcaca tgctgaccga gccggcgttc aacgcattgc
     4621 tcaagacgct cgaagagccg ccgccgcacg cgatctttgt cctcgccacg accgatccgc
     4681 agaaagtccc cgtaaccatc gtgtcgcgct gccagccgtt cgccttccgg cgcctgacgc
     4741 tggacgagat cgtctcacgc ttacggcaac tggtggatgc cgaagggcta catgccgacc
     4801 ctgttgcact gcagatagtc gcccgccagg ccaccggctc gatgcgcgat gcgatcagct
     4861 tgctcgatca actcgccgcg gggagtgaag caatcaccgt ggagcgggcg caggacgtgc
     4921 tgggtgcggg cgcgctcgag cacgtcatgg cgctggccga cgcgctggcc tcgggagacg
     4981 cggccggtgg gttgagcgcg atcaataccg cgatcgaccg gggcgcggat gcgcgccagc
     5041 tcgcgcggca gattgtcgaa tatctgcgcg ggctgatgct gatcgcgttt ggcgatacct
     5101 cgcaagtcaa cgtcgcgagt gagcagcgca ggcatatgga ggagcaggcc acgcggctgg
     5161 aggcggatcg cctgatccgg gcgacgaagc tgttcaacca ggccgcgtcg gagctgaagc
     5221 cgggctggca gccgcagctt ccgctcgaat tggcgctcgt cgacgcgctg aatcgaccgg
     5281 cccagccggc gcaagcggcg cctgcgccgg ctcagacgac tcgtgcgccg gccccgtccg
     5341 cgccggcgcg atctcaggcg gccacggcgt ctgttgctcc aggctcgccc gatccgatcg
     5401 aatcgacccc gccggaggcg ccggcgacgg gcctggcttt cgacattgtg tcggatcaat
     5461 ggccggcgct gctggccgag ctgcgatcgc gcagcgtgca aacatgggga ttgatgaact
     5521 cgtgccaact gatcggcgtc gaaggcgata cgatcgtcct acagtggcca tcggcgatgc
     5581 tgcgggacaa gttcgagaaa ggcaaagaca agcgcctggt cgaagacgtg ctgagcgaga
     5641 tgatcggccg gcgcgtgcgc acgcgctgca tcgtggacga cgatcccgtc gtccaggaag
     5701 cgcgccgtct cggcgcgcag gtccgtttag tgtccgacca atgagtgtgt tgtgcaagac
     5761 agtgtctgtc aggcaagcga tattggagat tggtaattgg cattagcgat aagccatcag
     5821 cgatacgcga tacgccatag gagaactcga tgagcaaaaa aggtggtttc cgaatgccgc
     5881 agggcggtcc caaccccgcc aacctgatgt cgcagctcca gaagctgcaa gacgagatgg
     5941 ccaagacgca ggacgcgcta gccgacgaga cgatcgaagt cacggcgggc ggcggcgcga
     6001 tcacaatcgt catcaccggc cagcagcgcc ttagatcgat caagctttcc ccggaagtcg
     6061 tcgatccgag cgacgtcgag atgctgcagg acctgatcgt cgccgccgtc aacgaggcga
     6121 tcgagcgatc gcagatgtat gcggcggaga aggtgaaggg cctgacgggc gggctgggcc
     6181 tgccgccgct ccgctagatt cgcgacccat gacgatggac gaacgaggtt cgtcgatcgt
     6241 caatggtctt tcgtctctgc catggacacc acacccaaag ccgttactcg attgatcgaa
     6301 gcgttcgcgc aactgccggg catcggcccg aagtcggcct cgcggctgac gtacttcctg
     6361 atcagggatg cttcgggcgt tgtcgagtcg ctcgccgaag cgctgcgcaa tctgaagacg
     6421 agcacggtct tttgctcgac gtgcttcaac attgccgata ccagcccgtg cgctgtgtgc
     6481 gccagcgagg agcgcgacgg caccgtgatc tgcgtcgtcg aagagccgct cgacgtgcag
     6541 gcgatcgagc gcacgcgcga gtacaagggc cgctatcacg tgctgcacgg cgcgatctcg
     6601 ccgatcgacg gcgtcgggcc ggacgacttg cgcatccgcg agctgctcaa ccgcgtggcg
     6661 acgggtgcgg tgcgcgaagt catcctcgcc accaacccca gcctcgaggg cgagaacacg
     6721 gcgatgtaca ttcagaagaa gctcgccgga atgggggtga aggtgacgcg cctggcgcgt
     6781 gggctgcccg tcggcgggga cctggagtac gccgacgaga tcacgctggc cagagcgctg
     6841 gcgggccgca gcgagatgtg acacacggaa tggacgacac atccgtattc gacaaccttt
     6901 cagccgaacc gcgtgatatg gtcgggttat tcgatcgtat caagcgggcg cggtcgtcgc
     6961 tgaaacagac ctatgctcac ctcagcgacg ctcaaatggt cgcgcccgga gcggacggcg
     7021 gctggtcggt caaggatcac ctggcgcatc tgacgacgtg ggaggccggc atcgccgcgc
     7081 tgctgcgccg cgagccgcgc tgggcggcga tggggctgga cgacgagact gtgcgtcaga
     7141 acgacgaggc gggggtcaac gcagtcatcg atcggcacaa caaagatcgc gcgcgggcgg
     7201 atgtgctcgc cgatttcgag cagacacatc gtgacttgct ggccgtgctg gggcagttga
     7261 ccttcgacga cctgctcaag acgtattcat actatcagcc aaatgaaccc ggcgtgggca
     7321 gcggccggcc gatcctggca tggatcgctg gcaacaccta tgagcattac gccgagcacg
     7381 agaactggat tcgggctgtg gctgagtacg tgaccggggg aggtcgggca ggatgaagaa
     7441 gcgggaagcg aacaagatcg ctggcatcag cgatgaggcc gtcaaagcaa ggaccggcag
     7501 aacgtgggcc gagtggatca gggttctcga caaggccaaa gcctacgaga tgcctcacaa
     7561 ggacattgcc atctacctga gcgagaagca ccaggttccc gactggtgga cccagatggt
     7621 cacggttggc tacgagcagg cgcgcggact gcgcgagaag cacgagcggc ctgaaggcta
     7681 tcaggtcagc gcgagcaaga cgctggacgt cccggtcggg acactatacg cggcctgggc
     7741 ggacgaggcc atgcgcgccc gctggctgaa gcgcaagtcg ctcgccattc gcggcgcgac
     7801 gccaccgaag acgatccata tcacgtggac gggcaagagc agcgtcgaag cccgcttcta
     7861 cgccaggggc caggccagga gccaggtgac cgtgcagcac agcaagctgg tgagtgcgga
     7921 ggacggtgag aagagcaagg catattgggc gcaggcgctg gacaggctca agcgtgtgct
     7981 ggaggggtag actcagtaga tcacgggttt gattcgtagt agcgacttta gtcgctggct
     8041 ggcttggacg actgaagtcg tcactacgaa atcgtgatct gctgagactc cactgctgtt
     8101 gagcggttat accaccccga ctaccggata cgccatcgat caacgcagag aaacatgacc
     8161 ggctttagtg attctctacg cggttacgcc tacgacatcc acaagcgaga caacttcacg
     8221 tgccggtatt gtggcgccga cggcacgaaa tcttttgata cctggctcac cttgacggtc
     8281 gatcacctgg tgcccaaagg cgatcccaat cgagacaatc cggattacat ggtgacagcc
     8341 tgcggtttct gtaacacggc cgataatcgc tatttcgatt tggttgaaca acgcggtctg
     8401 aagctggatg gcctgacacc tgacgaactc gtggcacagc gcttgcccta cgtgcagcgc
     8461 acacgacaga gttatcggag cttctgggaa acaaatgtca ggaagtaatc gcaaagcatt
     8521 ccggtcgacc aagcgccaga aacaggcggc ttgatatttc cggtctttgt cagagacacc
     8581 atgtctcccc aatcaaagag cagcaggaac aatgagcacc gggaaaagca ctgataacca
     8641 gccacccccc gatgagatgg acaagttcat ccgtgcttat ctgcgcggag cagaactgcg
     8701 cggcgctttc ctgagcggag ccgatctctt cgacgcagac ctggccggcg cagatttgag
     8761 aggcgcagac ctgggcggtg ctttgctcag cgatgccaac ctgtgccggg ccgatttgag
     8821 tggggctaac ctcgtcgggg cagacctgag cgaagcgaac ctgtgtgggg cggatttgcg
     8881 tgacgcggat ctgaatgggg cagacctgaa cggcgcccgt tacgatcgtc gcacggtttg
     8941 gccggaagac ttcgaccctc agtcagccgg cgcagtcgag gtcgcagatg cgccatagga
     9001 taaacattca agccaccacc tcaattacct atctggcgtg ggggcatata gaagtcaccg
     9061 ttgacggcca tgcccacaag ttcaaggact gcaaagtctg gcccggcggc gcgaaagcgt
     9121 gggattggac gatgacgggc acacgacatc ggccgggtat ccaacccgcc gacatcgaag
     9181 aaatcataga ccagggtgtc gaagtgatga ttttgtcgcg gggtatgcag ctcatgctcc
     9241 acacctgccc ggagacggag caactgcttc gctcccgagg aatcgagtat catatcgaag
     9301 aaaccgggcg agcggtagag ttgttcaacg acttgatgaa acgaggccag aaggtcggcg
     9361 gcgtgttcca ttcaacctgt taaggagaca tcatcgcggc aggggtcctt ctcctgcttt
     9421 cacgttaacc acgcatggtt gcatgaactt cttcgacccg aagccggctg ccgaaagata
     9481 tgccagaggg cgccccttct tccatcccct ggtcatcagg cggatcaagt cgtatctgtc
     9541 cctcatcgag ccggtctcac gcgccatcga cgtcggctgc ggcacgggtc tatccaccat
     9601 cgccctcaaa gagatagctc gtcacattgt gggcgtggac atttcatcgg agatgatcgc
     9661 cctggctccg caggattctg aaggatattt catgcgccgc gccgttctcc tgttgctggt
     9721 gattg
//
LOCUS       DBNHXB010000165         4990 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1715067, whole genome shotgun sequence.
ACCESSION   DBNHXB010000165 DBNHXB010000000
VERSION     DBNHXB010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1715067"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2737)
                     /locus_tag="AC162G_06470"
     CDS             complement(<1..2737)
                     /locus_tag="AC162G_06470"
                     /inference="COORDINATES: protein motif:HMM:NF051532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="golvesin C-terminal-like domain-containing
                     protein"
                     /protein_id="HIR5075490.1"
                     /translation="MIRAVSSRILMAVGGGLLMAAVTLACFHTPVATGAGDAPPSTTP
                     VSPASGGTGGVVCFVAEGATACVERFGIQSIGTLTDQVRSLMQAMVDGPTAAERSRGI
                     RSALPAGAALADVMASPERAVVRFDLPPAYLASFDAGDAEDVNEQVATTLTPFNFARI
                     DVEARDPDRPDTFRLLSTFLPPIIIPTKPGEPSSSQPPPSSLTGGQPPEYGNPQASGG
                     LRGKTVFVSAGHGWYWHATQGRYRTQRPHYPSAPYPSGEGIIEDFNNAEIVNQYLLQY
                     LWNAGADAWTVRERSLITAMHIVDDVGPTFSAEGGWLTGSTGGWGSGGILSGTYRYAT
                     TVTSGATATATWTFTPALGAEYAVYVWFPNAANRTAAARYAVEHAGGTTVLTITQARD
                     GIDWRYIGTYPFRGGAAYRVRLTNQSVAPGLDVLADAIRIGGGVGDFAPAGIPPSFKP
                     RWEEQALRYAQWVGLPDAENLNDVIVRPIFSEWEYEAGEDGIYISYHTNGFTGYNTTV
                     RGTETYVHSFEPTPNSDVLQDFVHAELLNDIHTGWEATWPDRGQKSRDLGELRLLEMM
                     PGVLIENGYHDNPQDVEAEKDPRFNLLSARAIYKSIVRYWNSQDPNVPLVFLPEPPAH
                     LRARNSGPGQVTLEWRPGPTDTTGLLGHMATAYRVYTSTDGFGWSNAIDTSNTSLALS
                     GLAPGQLIFVRVTGVNAGGESFPTPVLAARVAGGGVAPALIVHGFDRIDRTGLIRQLD
                     GVEGYNRRMFLDRINRYDYIVQHAAVITLPFDSAVHAAVTDGYIGLGNYTVVDWIGGE
                     EQWPDVVLNPTDQSLLQAFLDGGGALLISGADIGTDLAANGAGPSFYTSTLHAAYVDD
                     DASTYNIVAVSGAIFEGLSPFSFDDGTHGTYDVDRPDVFTPLGGASPALMYNGGLG"
     gene            complement(2851..3501)
                     /locus_tag="AC162G_06475"
     CDS             complement(2851..3501)
                     /locus_tag="AC162G_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075491.1"
                     /translation="MQSNRSVRVGSWTTATAVAVVVLAIAEIVVLVGNVEFQFSNLLT
                     LLALILALLIALVILLAGRLGGPGLRPGEGEPAEKRFPDKGVTVTVPWQGRTVDVVRL
                     PLLPLERFVSDTDEFKPKRLVMNFEVVDAKDTDSVVTDFEPPFELRIDIPEEYLAGIK
                     HNYDAIEMGFWDGCRWIKFTREKHQFQVRPNDPPGTGAVAIAMISNWADPPCGYGP"
     gene            complement(3504..>4990)
                     /locus_tag="AC162G_06480"
     CDS             complement(3504..>4990)
                     /locus_tag="AC162G_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5075492.1"
                     /translation="TDIYAALTSDEQLIMGALSVFPNPVERAAAEHVLEAEGVSGMVP
                     KIEALVNRHLIGAGEDERLRCHALVREYCYDILTRRDRERFHQRAAEYFEGERGYLAA
                     AYHHFERRAYAQALDLLTTQSQAIINSGGAAALVERLTRFQQHVVSLDQKVALQRALG
                     DGHQIRGEYPAAIDAYHAALEDAPDGQRRAELQRLIGSTYLKIGEYGRALEYAMKSLE
                     ISTATGEQAGMASAHHNSGWSLYRIGRLEQAREHFEASHRLGQALGDEILVAEVGMGL
                     GLVAWKEGRLDEARARFEESRRTFRECNDRAKEAYALGNLGVLYSALNDPDRQLSYYR
                     QALQIQEEIGDVDGLRWSYNNLGYVHHSIGEYPHARYYYEQLARLAQDIGHKRMLSMA
                     QTGLADAYLAEGDAQAALERATDAHRLAQEVGPGLELGISSRALGDVWLRLGDDRRAQ
                     AFFEQCMPLLEAVKEEGELARAHKGLKAIKDRLRTDPAAQQTGG"
BASE COUNT          869 a         1685 c         1546 g          890 t
ORIGIN      
        1 cgcccaggcc gccattatac atcagcgcag gcgacgcgcc accgagcggc gtaaaaacgt
       61 cgggccggtc gacgtcgtaa gtcccatgcg tgccatcgtc aaatgagaag gggctgagac
      121 cctcgaagat cgcgcctgat acggccacaa tattatatgt gctcgcgtcg tcgtcgacgt
      181 acgcagcatg cagcgtgctc gtgtaaaacg aggggccggc gccgttcgcc gccaggtcag
      241 tgccgatgtc ggcgccgctg atcaggagcg cgccgccgcc gtcaaggaac gcctgcaaga
      301 gtgactgatc ggtcgggttc aaaacgacgt ccggccactg ctcctcgccg ccgatccagt
      361 cgacaactgt gtaattgccc aggccgatgt agccgtcggt gacagcagca tgcaccgctg
      421 aatcgaaggg cagcgtgatg actgccgcgt gctgcacgat gtagtcgtag cggttgatcc
      481 ggtcgaggaa catgcggcgg ttgtagcctt ccacaccgtc gagctgcctg atcagtcctg
      541 tgcgatcgat ccgatcgaac ccgtgcacga tgagggcggg cgcaacgccg ccgccggcga
      601 cgcgcgcggc caggacaggc gtcgggaacg attctccgcc cgcattgaca cccgtgacac
      661 gaacgaatat caactgcccc ggcgcgaggc cgctcaacgc cagcgacgtg ttcgacgtgt
      721 cgatcgcatt gctccacccg aatccatccg tgctggtgta tacccggtat gcggtcgcca
      781 tgtgcccgag caggccggtc gtgtccgtcg ggccgggccg ccactcgagc gtcacctggc
      841 ctggcccact gttgcgagcg cgcaggtgcg ctggcggctc cggcaggaac accagcggca
      901 cgttcggatc ctgcgagttc cagtagcgga cgatgctctt gtagatggcg cgcgccgaca
      961 gcaggttgaa gcgcgggtct ttctcagctt cgacatcctg cgggttgtcg tgatagccgt
     1021 tttcgatcag cacgccgggc atcatctcga gcagccgcaa ttcgcccagg tctcttgact
     1081 tctggccgcg atccggccag gtcgcttccc agccggtatg aatgtcgttg agcagctcgg
     1141 cgtggacgaa atcctggaga acgtcgctgt tcggcgtcgg ctcgaacgag tggacgtacg
     1201 tctccgtgcc gcgcaccgtc gtgttatagc cggtgaagcc gttggtgtga tacgagatgt
     1261 aaatcccgtc ctcgcccgcc tcgtactccc attccgagaa gatcggacga acgatcacgt
     1321 cgttcaggtt ctcagcatcc ggcaggccga cccattgggc gtagcgcagc gcttgctctt
     1381 cccagcgcgg cttgaagctt gggggaatgc ctgccggcgc gaagtcgccc acgccgccgc
     1441 cgatgcgaat cgcatcggcc agcacgtcga gaccgggcgc gactgattga ttcgtcaacc
     1501 gcacgcgata cgccgcaccg ccccggaatg gatacgtgcc gatgtagcgc cagtcgatcc
     1561 catcgcgcgc ctgggtgatc gtcagcaccg tcgtgccgcc ggcatgctcg acagcgtagc
     1621 gcgccgccgc cgtgcgattc gccgcattgg ggaaccacac gtacaccgcg tactcggcac
     1681 ccagcgcggg tgtgaacgtc cacgtcgcag tggccgtcgc gccggaggtg accgtggtcg
     1741 cgtagcgata cgtcccgctg agaatgccgc cgctgcccca gccgccggtc gagccggtca
     1801 gccagccgcc ctctgcggag aacgtcgggc cgacatcgtc gacgatgtgc atcgccgtga
     1861 tcaggctgcg ttcgcgtacc gtccacgcgt ccgcgcccgc gttccacagg tactgcagca
     1921 ggtactggtt gacgatctcc gcgttgttga agtcctcgat aatgccttcg ccggacgggt
     1981 acggggcgct cgggtaatgc ggccgctgcg tgcgatacct cccctgcgtc gcatgccagt
     2041 accagccgtg tcccgcgctg acgaagacgg tcttgccacg caggccgccg gaggcttgcg
     2101 ggtttccgta ttcaggaggc tgcccgccgg tcaaggatga aggaggcggc tgactgctgg
     2161 atggctcgcc cggcttggtt gggatgatga tgggcggcag gaaggtcgag agcagacgga
     2221 aggtgtcagg ccgatccgga tcgcgggctt cgacgtcgat gcgcgcgaag ttgaacggcg
     2281 tgagcgtcgt cgcaacctgc tcgttcacgt cctcggcgtc gccagcgtcg aaggaagcaa
     2341 ggtaagcagg gggcagatcg aatcggacaa ctgcgcgttc gggcgacgcc atcacgtcgg
     2401 ccagtgcggc cccggccggc agcgcggatc ggatgccgcg cgatctttcg gccgccgtcg
     2461 gcccgtcgac catcgcctgc atcaaggaac gcacctggtc ggtcagcgtg ccgatcgatt
     2521 ggataccgaa gcgctcgacg cacgccgtcg cgccctcggc gacgaagcac accacgccgc
     2581 cagttcctcc cgatgcgggc gaaacgggtg tggtcgacgg aggcgcatcg ccggcgccgg
     2641 tcgccacggg cgtgtggaag cacgccagcg tgaccgcagc catcagcagg ccaccgccca
     2701 cagccatgag aatgcgcgag ctgaccgcac gaatcatgtg agactccctc ccccggtcgc
     2761 ccattataac agaccgcgcc ggccgcagag cgaccggcgc ggggtacgga atgacaacgg
     2821 cttcaccaag gcattggctc agaccaggca ctatggccca tagccacagg gtggatccgc
     2881 ccagttcgaa atcatggcaa tcgccacggc cccggttcca ggcggatcgt tgggacgcac
     2941 ctggaactgg tgcttctctc tggtgaactt gatccagcgg cagccgtccc agaagcccat
     3001 ttcaatcgcg tcatagttgt gcttgatacc cgccaggtat tcttccggga tgtcgatgcg
     3061 cagttcgaac ggcggctcga aatcagtcac cacgctgtca gtgtccttgg catcgactac
     3121 ttcgaaattc atcacgagcc gcttcggctt gaactcgtcg gtgtccgaca cgaacctctc
     3181 cagcggcaag aggggcagtc gcacgacgtc gaccgtgcga ccctgccacg gcaccgtgac
     3241 cgtcacacct ttatccggga agcgcttctc ggccggctcc ccttccccgg gccgtaggcc
     3301 cggcccgccc aaccggccgg ccagcaaaat gacgagtgcg atgagcagcg cgagaatgag
     3361 cgctaacagc gtgagcagat tggagaactg gaattcgacg ttgcccacga gcacaacgat
     3421 ctcggcgatg gccaggacga caaccgcgac cgccgtggca gtcgtccagg acccgaccct
     3481 gacactcctg ttgctctgca tgtttatcct cctgtttgtt gggctgccgg atcagtgcgg
     3541 agccgatcct tgattgcctt cagtcctttg tgtgctcttg ccagttcgcc ttcttccttc
     3601 accgcttcaa gcagcggcat gcattgctcg aagaaggcct gcgcccgccg gtcatcgccc
     3661 agccgcagcc agacatcgcc gagggcgcgg ctgctgatgc ccagttcaag ccccgggccg
     3721 acctcctgag ccagccggtg agcgtccgtc gcccgttcca gcgcggcctg cgcatcgccc
     3781 tctgccagat acgcgtccgc cagccctgtc tgggccatgc tcaacatccg cttgtgcccg
     3841 atatcctgcg ccaggcgcgc cagttgctcg tagtaatacc tggcgtgcgg atactcgccg
     3901 atcgaatggt gcacataacc caggttgttg taactccagc gaagaccgtc cacgtcaccg
     3961 atctcctcct gaatctgcag cgcttgccgg taataggaca gttggcgatc cgggtcattc
     4021 agcgcactgt acagaacacc caggttacct aaagcatagg cctctttcgc ccgatcgttg
     4081 cactcgcgga aggtgcggcg gctttcttca aagcgcgctc gcgcctcgtc cagccgtccc
     4141 tccttccagg cgaccagtcc cagccccatg cccacctcgg cgacgaggat ctcatcgccc
     4201 aacgcctggc cgagccggtg gctggcctca aagtgctcgc gcgcctgttc caaacgaccg
     4261 attcgataca gcgaccaacc gctgttgtga tgcgcgctgg ccatacccgc ctgttcgccc
     4321 gtcgcggtgc tgatctccag gctcttcatc gcatattcca gcgcccggcc gtattcgccg
     4381 atcttcagat acgtgctgcc gatcagacgc tgcagctcgg cgcggcgctg cccatcaggc
     4441 gcgtcttcca gcgcggcgtg gtaggcgtcg atcgccgccg gatactcgcc tcgaatctgg
     4501 tgcccatcgc ccagcgcccg ttgcaaggcc accttctggt cgaggctgac gacgtgctgc
     4561 tggaagcgag tcaaacgctc gaccagcgcc gccgcgccgc cggaattgat gatcgcctgg
     4621 gactgcgtgg tcagcaagtc cagcgcctgc gcgtacgccc gtcgctcgaa gtgatgatag
     4681 gccgccgcca ggtaaccgcg ctcgccctcg aagtattccg ccgcacgttg atggaaacgc
     4741 tcacgatccc ggcgagtcag gatgtcgtag caatactcgc gcaccagggc gtggcagcgc
     4801 agacgctcgt cctcgccggc gccgatcaga tgccggttca ccagcgcttc gatcttgggc
     4861 accatccccg atacgccttc cgcctctaag acatgctcgg cggctgcccg ctcgaccggg
     4921 ttgggaaaca ccgacagcgc gcccataatc agttgctcgt cggacgtcaa cgcggcgtag
     4981 atgtccgtca
//
LOCUS       DBNHXB010000166         6415 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4567497, whole genome shotgun sequence.
ACCESSION   DBNHXB010000166 DBNHXB010000000
VERSION     DBNHXB010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4567497"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="AC162G_06485"
     CDS             <1..271
                     /locus_tag="AC162G_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5075493.1"
                     /translation="RTGEAALERLAATAPDLVVLDLKLLPHVSGTDVLLRIRADPRLA
                     ETRVIVVTGHPDLADAVRDRADLVLLKPVDVNQLSDFVLRLRPGN"
     gene            complement(442..837)
                     /locus_tag="AC162G_06490"
     CDS             complement(442..837)
                     /locus_tag="AC162G_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5075494.1"
                     /translation="MRRRTIVIVCLLLALSLALAACGGGAAPAATSAPAGPVGDASKG
                     AEKFAGTCAACHGPDATGITGLGKNLTTSEFVKGKSDADLLAFVKVGRPASDPANTTG
                     VDMPPRGGNPVLTDQDLADIIAYVRTLQK"
     gene            complement(911..1174)
                     /locus_tag="AC162G_06495"
     CDS             complement(911..1174)
                     /locus_tag="AC162G_06495"
                     /inference="COORDINATES: protein motif:HMM:NF017394.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HIR5075495.1"
                     /translation="MSKFDDIGDMLAYHARHPELEVRARFVHDYNTEECILGETAFYV
                     RSSQIVSPMGHGIAAFRDRSAAETFAGGIGASVLTFDALRAAR"
     gene            complement(1174..1899)
                     /locus_tag="AC162G_06500"
     CDS             complement(1174..1899)
                     /locus_tag="AC162G_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR5075496.1"
                     /translation="MGVSPESLHSIPIFAGVGGLLLDGIAAHTQAKTYSGRKLIVQEG
                     QLCIGLHIVVDGRVKLFRRSDDREQIMVVLGRGEVVDAAPLIDGGEHRVSAQPLGKAT
                     VYFIDRATMLATIDQCPSVREALLNHMAERLRTFAELASDLAFKDATARVANAILVCA
                     EEGSHTDATGTRLDRKLTRQELAARAGTVREVVLRSLKRLQQQGLIADEAGQIVILDR
                     AGLQRAAQGEAAHASPPHSPRTA"
     gene            complement(1997..2674)
                     /locus_tag="AC162G_06505"
     CDS             complement(1997..2674)
                     /locus_tag="AC162G_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR5075497.1"
                     /translation="MIKIESLAAIPFFADFSAEELNGIAAMMTEHVYRPGEIIFLEGQ
                     AADGLFFVASGRVRVYKTSPSGKEQALCLVSPRTCFGGCPIFDGNISPVTAQAVDEVV
                     AYLLPREDAVERARDNLALSRAILRIFAGRLTHLTTVVDGLSFKCVTERIAEKLLAYA
                     DERGQATERGVEVHLDLTQEKLASLVGCAREVVTRSLLRLERLGAIEARGRHIVIIDR
                     EKLIQVT"
     gene            complement(2738..3310)
                     /gene="hpt"
                     /locus_tag="AC162G_06510"
     CDS             complement(2738..3310)
                     /gene="hpt"
                     /locus_tag="AC162G_06510"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR5075498.1"
                     /translation="MDAAYEPYLGEILIDAEAVQRRVAELAHRISDDYRGKMPLLVCI
                     LKGGIVFLVDLIKHIDIPHSVDFMAVSSYGAGARESSGAVRIIMDLEQDICDKHVLII
                     EDIVDSGRTLHYIYNLLRARNPASLRVCALLDKHERREIDVTLHYVGFDIPNKFVFGY
                     GLDLDEIFRNLPFVGVLKDELLPKETPPRG"
     gene            complement(3369..3950)
                     /locus_tag="AC162G_06515"
     CDS             complement(3369..3950)
                     /locus_tag="AC162G_06515"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIR5075499.1"
                     /translation="MDGQPGHGWIEVVCGSMFSGKTEELIRRVRRAKIARQKVQVFKP
                     ALDTRYAAEKVTSHNGLDTAAVPVKSTRTLLERVEPDTNVVAIDEVQFFDLDIVSVCQ
                     SLAERGLRVIVAGLDMDFRGEPFGPMPLLMAEAEQVEKLQAICVVCGTPASRTQRLID
                     GHPASYDDPIVLVGGSESYEARCRLHHEVPGRP"
     gene            complement(4165..4482)
                     /gene="rpmE"
                     /locus_tag="AC162G_06520"
     CDS             complement(4165..4482)
                     /gene="rpmE"
                     /locus_tag="AC162G_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016868067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR5075500.1"
                     /translation="MKKGIHPEWHPEAKVYCNGELVTTVGATVPQLNVEVWSGTHPFY
                     TGQQRIVDTEGQVERFVKRLERREGIAAVSKRKKAARRRERQAIAEYAEEGEKPASEA
                     VPE"
     gene            complement(4494..4766)
                     /gene="rpmA"
                     /locus_tag="AC162G_06525"
     CDS             complement(4494..4766)
                     /gene="rpmA"
                     /locus_tag="AC162G_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR5075501.1"
                     /translation="MAHKKGGGSSRNGRDSNAQRLGVKKYGGERVRSGNIIVRQRGTH
                     IKPGRNVGVGKDYTLFATVDGMVKFEPLPGNRTRVSVYPVEQEAGA"
     gene            complement(4864..5181)
                     /gene="rplU"
                     /locus_tag="AC162G_06530"
     CDS             complement(4864..5181)
                     /gene="rplU"
                     /locus_tag="AC162G_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR5075502.1"
                     /translation="MYAVFQVGGRQYRATEGDKIQVERLPQAVGEKVEFDRVMLISDG
                     EALSVGQPVLEGARVVAEVVEQGKGDKIIVFDYRPGGKRHRVKSGHRQNYTWLKVERI
                     IPG"
     gene            complement(5300..5935)
                     /locus_tag="AC162G_06535"
     CDS             complement(5300..5935)
                     /locus_tag="AC162G_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HIR5075503.1"
                     /translation="MDRQRVRITFARGDNVRYVGHLDMARTWERIVRRAGLPMAYSEG
                     FNPRPRLSFAAALPVGCTSDQEVLDVILSEPRDPAEIRDRLNQAAPDGITVLDVSEVP
                     YSAPALQTQTRAAEFAVTLLDPISPEGMRQRVQALLDAESIERTRRDKTYDLRPLILD
                     MSVEASETGQVRVGMTLKAEQSGTGRPDEVVAALGVDAAAVRIHRRRLIFA"
     gene            complement(6013..>6415)
                     /locus_tag="AC162G_06540"
     CDS             complement(6013..>6415)
                     /locus_tag="AC162G_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR5075504.1"
                     /translation="ESATRGKVGVVGQWLQVRDASGAQGYVAAWYVSTSKQAPVPPPS
                     GAGAPVGATTGTLMVQTTTPDVALRSQPVLSDQTLIKRMRFNSKLAVLEPAADAVRKV
                     GAVNQWLKVRDSTGTEGVVAAWYISVLPATG"
BASE COUNT         1136 a         2177 c         1900 g         1202 t
ORIGIN      
        1 tcggacgggc gaagccgccc tggagcggct ggctgcgaca gcgccggacc tggtcgtgtt
       61 ggatttgaag ctgctcccgc atgtatcagg gacagacgtt ctccttcgca ttcgcgccga
      121 cccacgcctg gcagaaacgc gcgtcatcgt cgtcaccggc catccggacc tggccgacgc
      181 cgtacgtgac agggcggatc tggtgctgct caaacccgtc gacgtgaatc agttgagcga
      241 cttcgtacta cgcctgcgtc ctggcaattg aagcgaccgc taacgacatt cgcgctattc
      301 gcgttgttcg cggatcaaag cctccggcat atcggccaaa tgaacgacgc ctccgactca
      361 accgtcgaag gcgtcgccat catcacgtac agacccccgg tttcttggag aaaccggggg
      421 tctggggagc taaactccag cttacttctg caacgtgcga acgtacgcga taatgtcggc
      481 caggtcctga tcggtcagca ccgggttgcc gcccctaggc ggcatgtcga caccggtcgt
      541 gttcgcagga tcgctcgccg ggcggccgac tttgacgaag gcgagcaggt cagcgtcgct
      601 cttgcccttc acgaactcgc tggtcgtcag gttcttgccc aggccggtga tgcccgtcgc
      661 gtccggcccg tggcacgcgg cgcacgtgcc ggcgaacttt tctgcgccct tcgacgcgtc
      721 gcccaccggc ccagccggcg ctgaggtcgc ggcgggcgcg gctccaccgc cgcaggcggc
      781 cagcgccagc gacagcgcga gcagaaggca gacaatgacg atggtgcgtc ttctcatgct
      841 gtgattctcc cttgcgtagt gtttgatgtg cgcaacacct ctgcgaccgg cgccgaggtt
      901 gaaccttggg ttagcgcgcg gcgcgcagcg cgtcgaatgt taacaccgat gcgccaattc
      961 caccagcaaa cgtctcggcc gcgctgcggt ctctgaacgc ggcgatgccg tgccccatgg
     1021 gtgagacaat ttgagaactc cgcacgtaga aagccgtctc gccgagtata cattcttcgg
     1081 tattgtaatc gtgcacgaac cgggcgcgca cttctaattc cgggtgcctg gcgtgataag
     1141 ccagcatgtc gccgatgtcg tcgaacttgc tcatcacgcc gtccgtggtg agtgaggcgg
     1201 cgacgcgtgc gccgcttcac cttgcgcagc ccgctgcagg ccggctcgat ccagaatcac
     1261 gatctggccg gcttcgtcgg caatcaagcc ctgctgctgc agccgtttca gagagcgcag
     1321 gacgacctcg cgcaccgttc cggcgcgggc tgccagctcc tgacgcgtga gcttgcggtc
     1381 gagacgcgtg ccggtcgcgt cagtgtggct gccctcctcc gcacatacga gaatggcgtt
     1441 cgccacgcgg gcagtggcgt ctttgaacgc caggtcgctg gccagctcgg cgaacgtgcg
     1501 cagccgctcg gccatgtggt tcagcagcgc ttcgcgaacg gacgggcact ggtcaatcgt
     1561 ggcgagcatg gtcgcccggt cgatgaagta gaccgtcgcc ttgccgagag gctgcgcgct
     1621 cacccgatgc tcgccgccgt cgatgagcgg cgcggcatcg accacctcgc cgcgcccaag
     1681 cacgaccatg atctgctcgc gatcgtcaga gcgccggaac agcttgactc gcccgtcgac
     1741 gacgatgtgc agcccgatgc acaactggcc ttcctgcaca atcagcttgc gccccgagta
     1801 agtctttgcc tgcgtgtggg ctgcaatgcc gtcgagcaga agcccaccga cgccggcgaa
     1861 tatgggaatg gaatgcaggg attcaggtga tacgcccatc gcctctcccc tttgtacatg
     1921 gaaatgcaaa ggggccgccc ttgccgtcaa gattagcaga aggcgacccc ttttgttagg
     1981 acgaaagtca cacttttcag gtcacttgga tcagtttttc acgatcgatt atgacaatat
     2041 ggcgtcctcg tgcctcaatc gcgccgagtc gttctagccg caataaggag cgcgtgacca
     2101 cctcgcgagc gcaccccacc aacgaagcca gcttctcctg cgtcaggtcg aggtggactt
     2161 ccacccctcg ctcggtcgcc tggcctcgct cgtcggcgta agccagcagt ttctcggcga
     2221 tgcgctcggt gacgcacttg aaggacaggc cgtcgaccac cgtcgtcagg tgcgtcaggc
     2281 gcccggcgaa gatacgcagg atggcgcgcg acagagccag gttgtccctc gcccgctcga
     2341 ccgcgtcttc tcgcggcaga agataagcca ccacttcgtc gacggcctgc gcggtgacgg
     2401 gagagatgtt gccgtcgaat atcgggcagc cgccgaagca cgtgcgcggc gacaccaggc
     2461 acagcgcctg ctccttaccg gacggcgacg ttttgtacac ccgaacccgg ccgctcgcca
     2521 cgaagaacag gccatccgcg gcctgccctt ccaggaagat gatctcgccc ggccggtaga
     2581 cgtgctcggt catcatggcc gcgatgccat tcaactcttc cgccgaaaaa tccgcgaaaa
     2641 aggggatggc agccagtgat tcaatcttga tcatcggaga ccttggctga gaggacgaaa
     2701 ctctgcgcgc ccatcatacg cccggctgcg cgagatgtca accgcgtgga ggagtctcct
     2761 tcggcagcag ctcgtctttc aacacgccga caaacgggag attgcgaaag atctcgtcga
     2821 ggtctagtcc gtatccgaat acgaacttgt tcgggatgtc gaatcccacg tagtgcagcg
     2881 taacatcgat ctcgcgccgt tcgtgcttgt cgagcagcgc gcagacgcgc aggctggccg
     2941 ggttgcgcgc ccgaagcagg ttgtaaatgt agtgcagcgt ccgcccgctg tcgacgatgt
     3001 cttcgatgat cagcacgtgc ttatcgcaga tgtcctgctc tagatccatg atgatgcgca
     3061 ccgctccgct cgactcgcgc gcgccggcgc cgtagctcga caccgccatg aaatcgaccg
     3121 aatgtggaat gtcgatgtgc ttgatcaggt cgaccaggaa gacaatgcct cccttcagaa
     3181 tgcagaccag cagcggcatc ttgcctcgat agtcgtccga aatgcggtgc gccagctcgg
     3241 cgacgcggcg ctgcacggcc tctgcatcga tcaggatttc acccaggtaa ggctcgtaag
     3301 ccgcgtccat atgtcctcct gttcaggctt tagacttcgg aagtcttctt aaagacttcc
     3361 gaagtctttc acggacggcc cggcacctcg tgatgaagcc ggcagcgcgc ctcgtacgat
     3421 tcgctcccgc cgaccaggac gatcggatcg tcgtacgagg ccgggtggcc gtcgatcagg
     3481 cgctgcgtgc ggctggcagg cgtcccgcac acgacgcaga tcgcctgcag cttctcgact
     3541 tgctcggcct ccgccatcag caagggcatc ggcccgaacg gctcgcctcg aaagtccata
     3601 tccagccctg cgacgatgac ccgcagcccg cgttccgcca gcgactggca caccgacacg
     3661 atatccaaat cgaagaactg cacctcgtcg atagccacga cgttcgtgtc aggctccacc
     3721 cgctctagca gcgtccgggt cgatttgacc ggcaccgccg ccgtgtccag cccgttgtgc
     3781 gacgtgactt tttccgccgc gtagcgcgtg tcgagcgccg gcttgaacac ctgaaccttc
     3841 tgccgcgcga tcttcgcccg ccgcacgcgc cgtatcagct cctcggtctt gccgctgaac
     3901 atgcttccgc acaccacctc gatccagccg tggccgggtt gaccgtccat ccatcctccg
     3961 ctcatgattt tcaagtgtat ccggtcagac catgcaggtc tccgtgatcc tgcctgccgc
     4021 taacatcaac gcacaagacc tgcccggtcc attgctcggg atattgaagt gtgagcagta
     4081 taacaaaaaa gggcagatga tcaactctgc ccttcctggt gtgcaccacc ttgaggcgct
     4141 tgccggctca gacggcccgg cgagctactc gggtaccgcc tcgctcgccg gcttctcgcc
     4201 ttcttccgcg tactcggcga tcgcctggcg ctcgcgccgg cgcgccgcct tcttgcgctt
     4261 cgagacggcc gcgatgcctt cgcggcgctc gagacgcttc acgaagcgct cgacctggcc
     4321 ttcggtatcg acgatgcgct gctggccggt gtagaacggg tgcgtccccg accacacttc
     4381 gacgttcaac tgcggcactg tcgcgccgac cgtcgtaacc agctcgccgt tgcagtacac
     4441 tttcgcttcc ggatgccact ccggatgaat gcctttcttc atgacagatt cctttacgcc
     4501 ccggcttcct gctcgaccgg gtaaacgctg acgcgcgtgc gattgcccgg cagcggctcg
     4561 aatttcacca tgccgtcgac cgtcgcaaac agcgtgtaat ccttgccgac ccccacgttg
     4621 cggcccggct tgatgtgggt gccgcgctgc cgcacgatga tgttgccgga tcgcacgcgc
     4681 tcgccaccgt atttcttcac gcccaggcgc tgagcgttgc tgtctcgacc gttgcggctg
     4741 gaaccgccgc ccttcttgtg cgccatgatc aacctctcaa cctagcatcc cgggttccct
     4801 gagttcctta agttccctca actgtcatga ccggcgttga gctactcaag ggactgaagg
     4861 aattcagccg gggataatcc gctcgacctt gagccaggtg taattctggc gatgcccgct
     4921 cttcacgcga tggcgcttgc cgccgggccg gtaatcgaac acgatgatct tgtcgccctt
     4981 gccctgttcg acgacctcgg ccacgaccct ggcgccctcc agcaccggct gcccgacgct
     5041 gagcgcctcg ccgtccgaaa tgagcatcac ccgatcgaac tcgaccttct cgccgacggc
     5101 ctgcggcagg cgctcgacct ggatcttgtc gccctcggtc gcgcgatact gccggccgcc
     5161 cacctggaaa accgcgtaca cgtaaatcct ccactgccaa gcgaacaatt cgcggccaaa
     5221 tgcagccggc cgcggagcaa atacattata ccgacttcaa ccaagttgtc aagcgaggat
     5281 cgacaagact tcggaagtct taggcgaaga tcaagcggcg ccgatggatg cgcacggccg
     5341 cagcatccac ccccagcgcc gcgacgacct catccgggcg gcccgtgccc gattgctctg
     5401 ccttgagcgt catcccgact cggacctgac cggtctcgct cgcctcgacc gacatgtcca
     5461 ggatcagagg cctgagatcg taggtcttgt cgcgccgcgt gcgctcgatg ctctctgcat
     5521 ccaggagcgc ctgaacacgc tggcgcatgc cctccggcga gataggatcg agcagcgtga
     5581 cggcgaactc ggcggcccgc gtctgcgtct ggagcgccgg cgccgaatag gggacttccg
     5641 acacgtcaag cacggtaatg ccgtccggcg cggcctggtt gaggcgatcg cgaatctctg
     5701 ccggatcgcg cggctcggac aggatcacgt ccagcacttc ctgatcgctg gtgcagccta
     5761 ccggcagcgc tgccgcgaac gaaaggcgag gccggggatt gaacccctcg gagtaggcca
     5821 tcggcaggcc ggcgcgccgg acgatgcgct cccatgtgcg ggccatgtcg agatggccga
     5881 cgtagcgcac gttgtccccg cgcgcaaatg tgatgcggac acgctgacgg tccatctgaa
     5941 cgaatcaatc cgtgtgatcc gcgttcatcc gcgtccaaaa gtcttggcga gcagagattc
     6001 cacgacgcgc ccctacccgg tcgcaggcaa gacggagata taccaggctg cgacgacccc
     6061 ctccgtcccg gtcgagtcgc gcaccttgag ccactggttg acggcgccca ccttgcgcac
     6121 tgcatctgcc gccggctcca gcacggccaa tttcgagttg aatcgcatgc gcttgatcag
     6181 cgtctggtcg ctcaaaaccg gctgcgagcg aagcgccaca tccggcgtcg tcgtctgaac
     6241 catcagcgtg ccggtagttg cgccgacggg cgctcccgcg ccggacggcg gaggcaccgg
     6301 agcctgcttc gacgtcgaga cgtaccatgc ggcgacgtag ccttgcgcgc cgctcgcatc
     6361 gcgcacctgc agccactgcc cgacgacgcc cacctttcca cgcgtggcgc tctcc
//
LOCUS       DBNHXB010000167         3568 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_709635, whole genome shotgun sequence.
ACCESSION   DBNHXB010000167 DBNHXB010000000
VERSION     DBNHXB010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_709635"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1840)
                     /gene="uvrA"
                     /locus_tag="AC162G_06545"
     CDS             complement(<1..1840)
                     /gene="uvrA"
                     /locus_tag="AC162G_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR5075505.1"
                     /translation="MSQDKLIVRGAREHNLKNITVEIPRDKFVVITGLSGSGKSSLAF
                     DTIFAEGQRRYVESLSAYARQFLGLMEKPDVDQIEGLSPAVSIDQRSASHNPRSTVGT
                     VTEVHDYLRLLFARVGTPHCPKCGREVFQQSSDQIVDAVMALPEGTRVQVLAPLVKDR
                     RGHHQQVFEDVRRMGYVRVRVDGRILDVNEEIELDRYKMHTIEAVVDRLVIKRSDEDR
                     SRLADSIETALRLGDGVVIVNNVSVEPPVDLLYSERFSCAYDGTSLPEIEPRTFSFNS
                     PHGACPACQGLGTRMEIDPKQVIRNPDLSLEGGAINVLEWGPRDGDEETYYRQILRAT
                     AAHYNIDMNVPVRTLSQAKLDIVLHGSGDDKIKVRYQNRNGDTRVYETTFEGVIPNMR
                     RRYAESTSDYIRSKIEEYMTYAPCPTCKGTRLKPEALAVTVNGSNIAQVSALPVVKAL
                     DWVNALTRPDSGNGSPALSERQRAIARQILKEIRARLQFMVDVGLDYLTLSRTAATLS
                     GGEAQRIRLATQIGSQLMGVLYVLDEPSIGLHQRDQARLIKTLQAMRDLGNTLLIVEH
                     DHDTMEAADWIIDLGPGAGVHGGEVIFEGTVKQLKADRKSITGAYLS"
     gene            2181..3230
                     /locus_tag="AC162G_06550"
     CDS             2181..3230
                     /locus_tag="AC162G_06550"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR5075506.1"
                     /translation="MRTLKGLVRLVLLAGAVFAALVLFASGSSSYSDPIGYYLGAAVA
                     VILAILLLPEALRGLIGGLIQWGILLGALGFTLMAVGSEDTLPLAAIGGLVSLFMIYR
                     LVVFPIRLNNNKKTVPATVVRISSKVRVGDAAMMRQDLPHGPVVAGTETYVLFDSRGQ
                     RIQVKLTPKQAREFAQEYSEGDTGRLTHSLGRLILWEPPTPERPLVSARHVSVFISYS
                     HQWTEDADYVAQFFRSQGLSVWFDKSQVRVGDRLTGAIEGAIRNSQYFVPLLSPEYWT
                     SEWCVKEFELAAAAKTKMLPIKVSAEPVTMPPHIRRLYRDVLGEPIFLDLRGRNPIQQ
                     LKELAGRMTSVPAAR"
     gene            3250..>3568
                     /locus_tag="AC162G_06555"
     CDS             3250..>3568
                     /locus_tag="AC162G_06555"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR5075507.1"
                     /translation="MTGHHFISYSSVDGRDFALRLSDALEAGPPHIPTWIDQRKLQTG
                     PDWDRQIVDAIRDCDSLIFVMTRDSVEDESVCKNEWVMALKYKKPVIAIRFHREADLP
                     FRLG"
BASE COUNT          581 a         1115 c         1162 g          710 t
ORIGIN      
        1 ccgacagata cgcgccggtg atcgattttc tgtccgcttt gagctgcttg accgttcctt
       61 cgaagatcac ctcgccgccg tgcacgccgg ccccgggacc gaggtcgatg atccagtcgg
      121 cggcctccat cgtgtcgtgg tcgtgctcga cgatcagcag cgtattgccc aggtcgcgca
      181 tcgcctgcag cgtcttgatc agccgcgcct ggtcccgctg gtgcaggccg atcgacggct
      241 cgtcgagcac gtacagcacg cccatcaact gcgaaccgat ctgcgtggca agccggatgc
      301 gctgcgcctc gccgcccgag agcgtcgccg ccgtgcggct cagcgtgaga taatccaatc
      361 ccacgtcgac catgaattgc aggcgcgccc ggatctcctt gagaatctgc cgggcgatgg
      421 cgcgctgccg ctcgctgagc gccggcgagc cgttcccgct atccgggcga gtcaaggcgt
      481 tgacccagtc gagcgccttc acgaccggca gcgcggacac ctgcgcgatg ttgctgccgt
      541 tgaccgtcac cgccagtgct tccggcttga gtcgcgttcc cttgcaggtc gggcaggggg
      601 cgtaggtcat gtattcttca atcttgctgc ggatgtagtc gctcgtcgat tctgcgtagc
      661 gccgccgcat gttcgggatc acgccctcga acgtcgtctc gtagacccgc gtgtcgccgt
      721 tgcggttctg atagcgtacc ttgatcttgt cgtcgcccga gccgtgaagg acgatgtcga
      781 gcttggcctg gctgagcgtg cgcacgggta cgttcatgtc gatgttgtag tgcgccgccg
      841 tggcgcgcag aatctggcgg tagtacgtct cttcgtcgcc gtcccgcggc ccccactcga
      901 ggacgttgat cgcgcccccc tccaggctca ggtcggggtt gcggatgacc tgcttgggat
      961 cgatctccat gcgcgtgccg aggccctggc aggccgggca cgcgccgtgc ggcgagttga
     1021 acgagaacgt gcgtggctcg atctccggca ggctcgtgcc gtcgtaggcg cacgagaagc
     1081 gctcgctgta cagcaggtcg accggcggct cgaccgacac gttgttgacg atcaccacgc
     1141 cgtcgccgag ccgcaacgcc gtctcgatcg agtcggccag ccgcgagcga tcctcgtcgc
     1201 tgcgcttgat cacgagccgg tcgacgacgg cctcgatcgt gtgcatcttg tagcggtcca
     1261 gctcgatctc ctcgttcacg tccaggatgc gcccgtcgac gcgcacgcgc acgtatccca
     1321 tgcgccgcac gtcctcgaaa acctgctggt gatgcccgcg ccggtctttg acgagcggcg
     1381 cgaggacctg cacgcgcgtc ccttccggca gcgccattac cgcgtcgacg atctggtcgg
     1441 acgactgctg gaatacctcg cgcccgcact tcgggcagtg cggcgtgccg acccgcgcga
     1501 acagtaggcg caggtagtcg tgaacctcgg tgaccgtgcc gaccgtcgag cgcgggttgt
     1561 ggctggcgga gcgctggtcg atcgacaccg ccgggctgag gccctcgatc tggtcgacgt
     1621 caggcttctc catcaatccc aggaactggc gcgcataggc ggagagcgac tcgacgtagc
     1681 ggcgctgacc ctcggcgaag atcgtgtcga acgccaggct cgacttgccc gagccggata
     1741 gccccgtgat cacgacgaac ttgtcgcggg gaatctcgac ggtgatgttc ttcaggttat
     1801 gctcgcgcgc gccgcgcacg atcagtttgt cctgcgacat agctgccttc gtgaaacgtg
     1861 acgcgtgatg cgtgactgtg cgttgtattg ggtttcctgg ggcattcggt ctgccaaacg
     1921 tatattatat cacgcttgac atcccccgtc cgggggattt ttatgatttg tgtgtacgca
     1981 aaaggggttt cgccgactta ccccaggccc gcttcccggg cgcggaggat cgcctgcgtc
     2041 cggtcggcga cctgcaattt gccgggaatg ctggtagcga gacagaatga gcagccacgc
     2101 gctggcggcg aaggaagatg atatgataga attgttaaag aagcatcccg gccctgacct
     2161 gttgttggta ggagggtatc atgcgcactc taaaaggtct tgttcgcctg gtgttgttag
     2221 cgggcgctgt ctttgccgcg ctcgtgctgt ttgcctcggg ttcatcctcg tattccgacc
     2281 cgatcggtta ctacctcggc gcggcagttg ccgtcatcct ggcgatcttg ctgttgccgg
     2341 aggcgctgcg tggcctgatc ggtgggctga ttcagtgggg catcctgctc ggcgcgctgg
     2401 ggttcacgct gatggcggtg ggttcggagg acactctgcc cttggcggcg atcggcgggc
     2461 ttgtctctct gttcatgatc taccggctcg tcgtgtttcc gattcgcctg aacaacaaca
     2521 agaaaaccgt gcccgcgaca gtcgtgcgca tctcgtccaa agtccgggtg ggcgatgcgg
     2581 cgatgatgcg gcaggacctg ccgcatgggc cggtcgtcgc cggcacggag acgtacgtct
     2641 tgttcgacag tcgcggccag cggattcagg tcaagctgac gcccaagcag gcgcgcgagt
     2701 tcgcccagga gtacagcgaa ggcgacaccg gccggctcac gcacagcctc ggccggttga
     2761 tcctgtggga gccgccgact cccgaacggc cgctggtcag cgcgcggcac gtgagcgtgt
     2821 tcatctccta ttcacaccag tggacggaag acgccgacta cgtcgcccag ttctttcgca
     2881 gccagggcct gagcgtttgg ttcgacaaga gccaggtgcg ggtgggtgac cggttgacgg
     2941 gcgcgattga aggcgcgatt cggaactccc aatactttgt gccactgctc tcgccggagt
     3001 actggacgtc cgagtggtgc gtgaaagagt tcgagctggc ggcggctgcg aagaccaaaa
     3061 tgctgccgat caaggtcagt gccgaaccgg tgaccatgcc gccgcacatc cgccgcttat
     3121 atagagacgt gctgggcgag ccgatctttc tcgacctgcg tgggcgcaac ccgatccagc
     3181 agctcaaaga gctggccggg cgcatgacga gcgtaccggc cgcacgatga ccttcgagag
     3241 gaggcagcca tgaccggaca tcatttcatc agttactcct cggtggatgg gcgggacttc
     3301 gcactgcgct tgagtgatgc gctggaagcc gggccgcccc acattccgac ctggatcgat
     3361 cagcgcaagc tgcagactgg cccggactgg gacaggcaga tcgtggacgc gatccgcgac
     3421 tgcgacagcc tgatcttcgt catgacgcgc gacagcgtcg aagacgaatc cgtgtgcaag
     3481 aacgaatggg tgatggcctt gaaatacaag aagcccgtga ttgcaatccg gtttcaccga
     3541 gaggcggatc tgcccttccg tctcggct
//
LOCUS       DBNHXB010000168        33227 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2554689, whole genome shotgun sequence.
ACCESSION   DBNHXB010000168 DBNHXB010000000
VERSION     DBNHXB010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 33227)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 33227)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33227
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2554689"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="AC162G_06560"
     CDS             <1..311
                     /locus_tag="AC162G_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075508.1"
                     /translation="YRRVVRQAAQTQTPGLLRAAFLIEDPTTCYSLSIWTSWDAIPRF
                     GTNVPRHVEAANRVFGRVCFSKDRGPEIWSTKWRLTSVSNNLNWEDFDLRALILGMGP
                     "
     gene            323..961
                     /locus_tag="AC162G_06565"
     CDS             323..961
                     /locus_tag="AC162G_06565"
                     /inference="COORDINATES: protein motif:HMM:NF018942.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HIR5075509.1"
                     /translation="MSRQMEISVLPHFAFASQLSLGIVFLLSALPKLRRPLAFVQSVV
                     EYKVLPAKVAYVFALALIPLEAFLAIAFLTGWWADAALPLATVMLIVFLMAVGTNLRR
                     GRKIDCGCFGNTSEQISPRTMARLLLLLTTVLLLVVFKGTGGASLPSRGAMTADVSTF
                     IYLLQTAFLAAFLILLAAWMLSLPEVVPLVRRWRWSQSSSGNTKTGDGVEGA"
     gene            963..1526
                     /locus_tag="AC162G_06570"
     CDS             963..1526
                     /locus_tag="AC162G_06570"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR5075510.1"
                     /translation="MDMSIPFYLSYAALWILVILHSLILLGVVRIVYQLQQTSVAAGS
                     RGLSSGKEAPQFSAVALSGEPISSTDFVGRLTALLFVSPSCPSCTAILEDDMGYLNHK
                     AQGNMIVICRAGREDCARLAEQHRLSMPVVADEDDQISGLYRISTVPTAVLINSNNRI
                     QSYGQPQREELEEMLEKAAEVGAQGVG"
     gene            1765..2340
                     /locus_tag="AC162G_06575"
     CDS             1765..2340
                     /locus_tag="AC162G_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075511.1"
                     /translation="MAGYKKKWLGEVIAYTLAGSVASASVGAVVGWLGGLLWPGQVSR
                     LGILVALAVGLIAIAREVGWLSFPLPELRRQTEGVWAKLFSGTVAATLWGFDLGLVFT
                     ARLTFSGVWLLVAVAMLVGEPAFGSALFVLYWLGRALSVWIAPLLMPDADATPWVLDV
                     IYRHYRLFQRIHVLGLVWSTVVLIVWLSKGT"
     gene            3439..4053
                     /locus_tag="AC162G_06580"
     CDS             3439..4053
                     /locus_tag="AC162G_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075512.1"
                     /translation="MGWYRGVRGELTVTGRRLDAPAPPAQGLYDSSGYGDIGFQAGAI
                     HFPSEGCWEITGRVGDASLTFVTLVVKVPFAAAWPHWGPEGLVIKDQDVTGLPKTIRL
                     IFGTPTWGEGGVTWGEGEVSIETTQGLWDNPDPYPDAATQQVTVNGQPGVCVQGAWDA
                     QGQWRDEADAGALEWAAEGFSYQISHIGLGLRCEDLLRIAGSPP"
     gene            complement(4243..5406)
                     /locus_tag="AC162G_06585"
     CDS             complement(4243..5406)
                     /locus_tag="AC162G_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5075513.1"
                     /translation="MTSAKDRAEFLRQVFPGLTDSWLSYMASTAHWRAYPAGTVICRE
                     GDYGDTFFIIESGTVEISKRLDGETSRILAREGAGEFFGELALVQGVPRVATATAIED
                     TELLEISKEDFSQYIDSNPAMAAAIMRAVAARLRDADQRAIAELRRKNAELREAYDDL
                     RREIKHRSGFLTTISHELRTPLTSVRGYTHLLKAGKLKDADFDHAVETLTRNVDRIVQ
                     LVNDILILEEIELVVPQMELINLESIVRGIVERQTSRAGESGLNILAEIETGLPRLRG
                     DESTLSQAINALLDNAIKFSPDGGDIVVKVRREGRHAVIVVTDPGVGMEPDVIGHIFE
                     WSHLRDEKGDHLFGGMGIGLPLVHAVVQHHDGTIDVESQPGRGSTFTVRLPFR"
     gene            complement(5474..7600)
                     /locus_tag="AC162G_06590"
     CDS             complement(5474..7600)
                     /locus_tag="AC162G_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5075514.1"
                     /translation="MPQARLRFVVVFAVVFILAAWLVFSRPSGSSVPAPTLTFPPAPR
                     VTITPPSLDELALRYPSLERLLRDPETDSVYKEFVLAYEQGGLVAAEELARRRGLLTP
                     DNEVRVTLVVDTTDTARLIDDLEALGVNVLGIYRDLIDISIPLNVIAGVAQSENPGAI
                     FEQIRDVEHVVGLRLNPPSVPQSARRSPRVQQSSSRSIVSEGVFAINAAEWHAAGFTG
                     QGIQIGVLDQGFDGFRDQLGRELPEHVIARSFVSGVEPDATGENHGTAVAEIVHDVAP
                     RAELFFAHYDGGDVSMGNAVDWLLEQGVQIISHSAGGLAGPMDGTGRDAELVDRVTAQ
                     DVLWINSAGNSADEHLRTTYSDADGDGFHDFKPESPLLAFRPVPDQTTQIVLNWDDWP
                     QADEDYDLYLLDRDGVVLASSRNIQAGNRAPVEQILYRFDDAGAYYVSVQANGTSRPA
                     RLDLYIHEARSMEFTTPSHSLATPADSRGALTVGAVDFRDNTLQPYSSYGPTGDGRQK
                     PEMLGPDGVSVATYAPEAFFGTSAAAPHVAGAAALIWSAHPGFDADDLRVSLLANTIP
                     VKSDAGPDATGSGVLRLPAPPRGVTKAEPATVENAPSGVAPAGSPTGAIAAVCAAGAG
                     ALAVISWLALRRRPALRKQGRPVADEDTVDCTTCGAQMPSTAQFCSQCGRSIDLAASR
                     ACTRCGARLRPRAAYCSNCGAEASIL"
     gene            complement(7607..8941)
                     /locus_tag="AC162G_06595"
     CDS             complement(7607..8941)
                     /locus_tag="AC162G_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5075515.1"
                     /translation="MDLVGKELGQYRITEKIGQGRLATVYRAYEEGLDRWVALKVIDP
                     KHASEPAFLARFKRGARTVAQLRHPHILSVYEFGERAGVVFLAMDYADGGALESHLTG
                     QPMEWSAAAALILPVARALAYAHSQGVTHQNIKPANILLARDDWPLLGDFQIARIADV
                     RRSLSADEDSIISPDYASPELAQAVEVDVRSDIYSLGVVLYQLVTGRRPFRGDTALDV
                     LMQHVNVTPESPCALNPSLPAMGEAIILRAMAKNPGSRYQSTEEVVNALQVALTQTAA
                     GSVDATYTPMIARSGTCSRCGASVNTLGRFCPKCGATLRAGVRAPTTPLPSLERAEAE
                     SQRGQGAHFVLASGSTILLPPKAELTIGRADKRNEIYPDIDLAPHGTPGEGVSRLHAR
                     LYQRGGVWVVEDAGSTNGTFLNGRRIAAGEEALLRDGDRLRCGQLILTFRSR"
     gene            complement(9036..9881)
                     /locus_tag="AC162G_06600"
     CDS             complement(9036..9881)
                     /locus_tag="AC162G_06600"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR5075516.1"
                     /translation="MRLRLNAAGLTDVGLEREHNEDAIFYLVTRNARDEPVGLFVVAD
                     GVGGQLAGELASHWAVASARESLRDLLSPPGPDQTEPAGIDRTPVLAPLRFGATHPAD
                     RDSLRQRVLRAVQAANRTVRAQAQAQPAEAGNAGTTLTMTLVYGDVAIIANVGDSRTY
                     LRRGGEMRQITRDHSLIAGLVASGNVKPEDAYTHPVRNVIVRSLGREAEVEIDLFVEQ
                     LHPGDTLLLCSDGLWEMVREDAVLASTIRGAATSMDAARALIDAANAAGGVDNISAIV
                     VRVET"
     gene            complement(9884..12391)
                     /locus_tag="AC162G_06605"
     CDS             complement(9884..12391)
                     /locus_tag="AC162G_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR5075517.1"
                     /translation="MTVSRDSPPSAGSTQLMPVAAAHLAIRWPDGAAQTMPLRKKTFT
                     IGRHPTNDIVVPIPTVSSEHALIEEHVIDGRRGHRVVDRGSRNGTFVNGRRASTVTLR
                     DGDIIRIGDALGNSVSLTYQAAHAVESVSLGQLDLAQADSLTIGRDPANDLHLDSPVV
                     SRRHARIERVPAGHVLIDLSSTNGTYVNGSRLQPNSGVTLRPGDVVHIGPFRLTYQPS
                     TLSQVVGAMQARIDALNLTRRAPPSGSPRSDGGREEGGVRLILRDVSLSILPREFVAI
                     VGGSGAGKTTLLDALSGVRRAEGRVLLNGDDFYAHYALYRGLLGYVPQDDIIHLDLPV
                     DRALRYVAQLRLPSDTSADEIEQRIRRVLAEVEMADHREQIVSSLSGGQRKRVSIGAE
                     LIAEPSLFFLDEATSGLDPGLEKKLMYTLRRMADAGRTIVLVTHATANIRQCDHVAFL
                     SGGRLVFFGPPAAALDFFGAADFADIYAEIERNPELWEERYRASTTAQQVLDARLSDL
                     PEAPRSATDISLPARPRVSFLRQFFILTQRTFELVLRDSVRLAVLLAVMPIIGVLLSL
                     LADPEALVGASEERVADILHETGRYSIAAQAQSVLMMLGLAAALLGMFVASFELVRER
                     AIYRRERMVNLRLAPYLGSKAAVLVGFALIQCLALIVAFGIRVPFPQAGTFLPGPLEI
                     YLTLALSAAAGTMIGLFISAIVNNAATAIYVVLFAVFAQIMFAGVIFPLPGATQGLSY
                     LTVTRWTVEALGSSVDLPGLNDLGRIEVTKTVESVDPVTNETVERPVTVRDRLRVDFN
                     VNYGHSPSYVIERWGVLVAFCLLFGGLSVLAQRRMDS"
     gene            complement(12420..12914)
                     /locus_tag="AC162G_06610"
     CDS             complement(12420..12914)
                     /locus_tag="AC162G_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012708.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR5075518.1"
                     /translation="MNDRYCPACGTLNVQAARFCHACGSRLRDAPGVTCTRCGVTALP
                     GDRYCDACGATLPGGAVLIHEDTGWRIAVPDRAENTIGREDVLSGARPDVDLAPYGAD
                     AYGVSRQHARLRRAENGYTLEDLNSVNLTYVNDERLEPGRKIVLADGDHILLGSLRLI
                     FRQA"
     gene            complement(12951..14852)
                     /locus_tag="AC162G_06615"
     CDS             complement(12951..14852)
                     /locus_tag="AC162G_06615"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR5075519.1"
                     /translation="MNCVVCGTHNPPEARFCRRCSTALPREAQPESSAADGAGDVATA
                     PPIDENTLGTRPFDSRTITRPLDGDEQAPPAVSPAGIDDTQPLPRPRYFFEALPVGAL
                     AGALPYEILSVISEDGSVNTYAAQDRHKRVRCPSCAYSGNSFGDEYCQQCGALLVGIE
                     PHHPQFIVKESIAADAIAIERRLADMRLYHGGALLPVDTFSESIAGTRRYYVVLPQPS
                     PLTGASLAAPQELLDILQWGMMLSESLAFLHGRNIAFGAADLSHVSLDNRMARWFDFT
                     SARIVSEGVQARKLFREDVTSLAALLFVLLTGQIYSPRVVLEPPSLTQLFRDVFNAQL
                     VSAASLVDRLRSALAEVRRPTTYDLRVGRLTDVGHHRQLNEDSLLTLDVGQVHRSVSR
                     PVGVYVVADGAGGHAAGDVASGIAIRVIARQALEGLLAQSIDEGPLHVDVPSWLRSTI
                     QAANEAVHRQRAVARTDMGTVLVMAVILDGEAHIAHVGDSRAYLIRGDDITQLTVDHS
                     LVQRLVDTGQITREEARTHENRNVIYKMIGDRSKIEPDVKRIALAPGDRVLLCSDGLC
                     QYVEDDDIHQIILAAPSPQEACQRLIDAANRAGGLDNITVILIQIDVLGKPEEQAEIE
                     IRQPSNSER"
     gene            complement(14849..15325)
                     /locus_tag="AC162G_06620"
     CDS             complement(14849..15325)
                     /locus_tag="AC162G_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012708.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR5075520.1"
                     /translation="MTLDPLLAIVVTLGLLGVLVAGFVVASRIRRRKAQLAAELAQPR
                     EPVAIAFLERLGEDEGPRIYELHRPLMGIGRGADNDIRIAPDVPGALTVSRRHAQIRR
                     EDVDYIVEDVDSTNGIRVNGLATRRNLLRDGYQVSFGSVEFVFRMSESPTASRMSR"
     gene            15465..16061
                     /locus_tag="AC162G_06625"
     CDS             15465..16061
                     /locus_tag="AC162G_06625"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012898785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HIR5075521.1"
                     /translation="MPARLSRHFFARPTLRVARELLGCRLVRRIDGQRLSGVIVEAEA
                     YVGENDLACHGRRGRTPRNAVMFGKPGVAYVYFTYGMHWMFNIVTEREGFAAAILVRA
                     LEPIEGIARMRERRKQAILRNLCSGPAKLTQALRIDRQDNGVDLCARGTDIWIEAGEP
                     LAAKQIGRSPRIGVGFAPEPWRSKPWRFFVRGNPFVSS"
     gene            16083..16877
                     /locus_tag="AC162G_06630"
     CDS             16083..16877
                     /locus_tag="AC162G_06630"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0030497 - fatty acid elongation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR5075522.1"
                     /translation="MGLLDGKKALIFGVANKDSIAWGIARMFHEHGATIGFSSIADLV
                     ERRVRPLAESIGSTFVEPCDVQSDEQIAAVFEKWKAAYGDLDILVHALAFAGREALAG
                     PYLDVTRAGFQLALDISAYSLTALVKAAHDVLRAGSSVITLTYYGSEKVVPHYNVMGV
                     AKAALEASVRYLAADLGPRGVRVNAISAGPIRTLSAKGVSGFTDMYKHFKDMTPLRQN
                     ITAEDVGKTAVWLASDLAHNVTGAVIFVDGGMNVLGLAVPPVTRDE"
     gene            complement(16913..17101)
                     /locus_tag="AC162G_06635"
     CDS             complement(16913..17101)
                     /locus_tag="AC162G_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075523.1"
                     /translation="MKIGVVSDTRDNREGLQMLLDRFLVGGARWLFHCGGLGGAELTL
                     SSVDSTLSLNTLTPITDD"
     gene            17484..18896
                     /locus_tag="AC162G_06640"
     CDS             17484..18896
                     /locus_tag="AC162G_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR5075524.1"
                     /translation="MKVKMILPALTEAKSPYWRPIKYSLFPPLGLATLAGYLSEDDEV
                     TIQDEHVETLHLDDEPDLVVIQAYITSAYRAYELADYYRRRGAHVTLGGLHVTSLPGE
                     AARHADTIFLGPGEDTWPAFLADFRAGRPQKLYRSQIRTLIGLPHIRRDLIKRHLYLV
                     PNSIVVSRGCPHTCDFCYKEAFFRGGKSFYTQAVDDALAEIERLPGRHLYFLDDHLFG
                     NPRFASALFDGMQGMGRLWQAAGTVKSVLAPGLLEKAAACGLRSLFVGFETLNPMNLR
                     EQRKYQNLNHDYTAAIRRLHGLGVMVNASFVFGMDDDDESVFDHTVDWAIEQGIETAT
                     FHILTPYPGTALYQRMMQERRILHHNWDLYDTRHVVFRPAKMSAEVLEAGYWHTYREF
                     YRWGSIFRSAWAKDAWSERLRHMVYAGGWKKMEFVWHGIIRLKRVSAMLPVLEATLGG
                     FRRLSERDARQRAGVSGAHV"
     gene            complement(18943..19788)
                     /locus_tag="AC162G_06645"
     CDS             complement(18943..19788)
                     /locus_tag="AC162G_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR5075525.1"
                     /translation="MPIGETFEVRKLDTPLDKITRTHAGRRSSSKTERKRGRYIKARP
                     SPGDPKDLAFDATLRAAAPHQPERRRQKIEGPALRVGRGDYQKKVRVRKAANLILFVV
                     DASWSMAVAERMAATKGAIMSLLTDAYQRRDRVGLIVFQKDQARLVLPPTSSIDLAQR
                     ALKDIPVGGKTPLSAGLTLAQRVLAREMQVHPDVAPLVIVLTDGAGNVSQGSASPQVE
                     AYNVAEQIAELDIRAVVINMEHQAFDQGLAARLAEHLDAPCYSLRDLKAESLYRTVKD
                     ELQSS"
     gene            complement(19899..20999)
                     /locus_tag="AC162G_06650"
     CDS             complement(19899..20999)
                     /locus_tag="AC162G_06650"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016851 - magnesium chelatase activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
                     /protein_id="HIR5075526.1"
                     /translation="MRIVFPFTAIVGQERMKRALILNAIYPQIGGVLIRGERGTAKST
                     AARALAALLPEIGVVENCRFGCDPGSPAEWCTECRERAAKGAELPQTTRRTRFVDLPV
                     SATEDRVVGTLDIEQAIQKGIRRFEPGVLAAANRGLLYVDEVNLLDDHVVDLLLDSAA
                     MGVNVVEREGISFSHPARFILVGTMNPEEGDLRPQLLDRFALAVDIRGIHDQQQRMQI
                     LERNLAFEQDPEGFREEWEPREHLLSEEIDRARQTLPMVRYTQADLAAIAGMVGSLGV
                     DGHRADLVILKGARAHAAKMGRDHITREDILLAAELALPHRLKRQPFQENGQVSMQDL
                     SERLEKAQSEFSGGEEAMLDEEGEEGSVKKKR"
     gene            complement(21105..21911)
                     /gene="surE"
                     /locus_tag="AC162G_06655"
     CDS             complement(21105..21911)
                     /gene="surE"
                     /locus_tag="AC162G_06655"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR5075527.1"
                     /translation="MTDRPLILITNDDGIQSRGLRAAAAACEPLGELLIAAPAMQQSG
                     AGRSMPPASEGRVYPEDVVVNGRTIVAYGIEGSPAQVVEHALFEIAQRPVSLVISGIN
                     YGENLGESITVSGTVGAAMEGASFGIPALAVSRQTEAALHLSHSADIDFSAAARYVRM
                     FSEAVLRWGLPAGVDLLKIEVPQDAMPDTPHRWTSISRQRYFYPTIPHRRRPTDPGPM
                     GYEMRIDEATLEAGSDVRAVVIDKVVSITPLTLDFTARVDFGQMESWFTR"
     gene            22079..22987
                     /locus_tag="AC162G_06660"
     CDS             22079..22987
                     /locus_tag="AC162G_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR5075528.1"
                     /translation="MPRHPYIAVEGVIGVGKTTLVRLLSKDFNAEPLLEVFEENPFLS
                     DFYSDRARYAFQTQIFFLLSRYRQQHDVIPRALMHSALISDYTFAKDALFARLNLGGD
                     EIAVYNRLHAALAEKIPKPDLLVYLRAGLDTLMARIAARDRPYERAMDRRYIESLRLA
                     YDGFVAAYTDCPVLAIDTDELNFVQDLGALDSVRERIRAALRQGTFQQALPHMPVTLT
                     ESDRSALSELQRAQAQAGETRRDAIAEFLSLTRSVGEVSAVLLDGGDQRMLRHSLAAC
                     LSRLMHLADATGVDLDEAYRAYSARK"
     gene            23117..23755
                     /locus_tag="AC162G_06665"
     CDS             23117..23755
                     /locus_tag="AC162G_06665"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR5075529.1"
                     /translation="MTKHFVAIAGNVGAGKSSLVGLLADKLGWTPFYEAVDENPYLSD
                     FYRDMRTWSFHSQVFFLSKRLRHHRRLLDHPSSVLQDRSVYEDAEIFAKNLYRQGNMD
                     ERDYGVYRELYEVLTLYLPPPDLVVYLQASVPTLLKRIAQRGREYERGISADYLASLN
                     ELYEDWIAGFTLCPVLSVPSDDLDFVNHDAHLALVASMIKDRLSSAPVVSMV"
     gene            23887..25092
                     /locus_tag="AC162G_06670"
     CDS             23887..25092
                     /locus_tag="AC162G_06670"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR5075530.1"
                     /translation="MLANDRHTSYLDHLTCSRCGAICAPDSLQTICPTCGRPLLARYN
                     LDAARTKLDRERMAARPDRMWNWRELLPVRDPQHIIYLGEGGTPMFHARRLGETLGLP
                     HLWIKDEGLNPTGSFKARGMAAAVSRAIELGARKFVVPTAGNAGAAAAAYGARGGAPV
                     HVYMPQDAPLANRAGAQAAGAEVILVDGLINDAGRMAATHAKERGWFDLSTTREPYRV
                     EGKKTMGYEIAHQLGWQAPDVVLYPTGGGTGLIGIWKAFEEMQALGWIDARRPRMVAV
                     QAEGCAPVHRALQAGADRCEAWPAAHTDAAGLRVPQPFADEMILDVLRRSGGASVVVS
                     DGAMRDAQECIRRREGISACLEGAATVAALRALLKTGLLQADEQIVCLNTGTNLHTAL
                     PPPKDGVDG"
     gene            25085..26245
                     /locus_tag="AC162G_06675"
     CDS             25085..26245
                     /locus_tag="AC162G_06675"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5075531.1"
                     /translation="MADTDADLALARSAGARSGAENAGAAAADSESLAARVAELERAY
                     ESLIEDSVVPILITDYDGKVLRANAAAARLAGVAKEALVGEPLNSSQLSIELRVLQTA
                     LPAARQNPDLGERLETRLASRNLSLDLHTRHMVYDGQPAIRWLVHDRTALQELERVRD
                     EQVYMIVHDVRNPLGNIISSLELLKDSLQDPTTTPAPGALVNIALRSSRRISLLVESL
                     MDMTRMEAGQFTLNTTTARLDTLIEHAVEFVKPTADRKRIALRTEIEPNLPPVLINVN
                     MIERVVVNLLDNACKFVTPGQAVTISARLGNGADVHISVNDEGPGIPPEDQRRIFEKF
                     SRGAGIGGQTPGTGLGLAFCKLAVETHGGRIWVESELGKGSTFTFTLPIEAS"
     gene            26249..27460
                     /locus_tag="AC162G_06680"
     CDS             26249..27460
                     /locus_tag="AC162G_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0002196 - Ser-tRNA(Ala) deacylase activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIR5075532.1"
                     /translation="MTDRLYYSDPTLRSFSARVVGRLEIDNHPAVILDRTAFYPEGGG
                     QPGDRGQINQARAIDVQAQDDAAHSVVHILSEPVAEDEVTCAIDWTRRFDLMQQHTGQ
                     HILSQAFVRLLDAETVAFHLNDDPNEGTATIDLDRAGLRPADVDAVEDLANQIVFENR
                     PVTARFVSREELASIPLRKPPTVDAAIRIVEIAEFDRSACGGTHVARTGEVGQIKIVK
                     LERRGPETRVEFRCGQRALADYRRKNDVINRAAAGLSIGFWELEEAAARLVAENKALF
                     KQLEEAQERLLEFEAHELWSNLREHGQLAAAMRVWHARDMNALRKLAKQVISRPRTVA
                     LLGSGGEKPALVFARSSDVALDMSKLVRDAAARIGGRGGGSPDFAQAGGPPASDEQVW
                     AAVEWAYEQLS"
     gene            27460..28311
                     /locus_tag="AC162G_06685"
     CDS             27460..28311
                     /locus_tag="AC162G_06685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5075533.1"
                     /translation="MEKIANNIYVSSSYRGVTVGAILTPMGVVCVDAPMLPAGARDWR
                     AKIASVTGAPIRYVINTDAHRDRVLGQQWLGGAVVATEAAADKMRTYGDTFRQQVADF
                     LSHHGAPEAAEEIALSLKMVQPQISFGGRLVLHATTPRIEIWNVGGANPASAWVVLPD
                     AGVVFTGDLLTLNVHPVMSESSTRVWLERLAGLRKPSFPAKKIVPGRGPVCRKADTQV
                     MSAYLRDVRSKVRALIKARRQKPDVASLVAAFLPRFPIPNGERERMQRRIRAGLEHVY
                     DELKQEK"
     gene            28353..29390
                     /locus_tag="AC162G_06690"
     CDS             28353..29390
                     /locus_tag="AC162G_06690"
                     /inference="COORDINATES: protein motif:HMM:NF017864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR5075534.1"
                     /translation="MSDERQISRYPTPLVEVTRGGLVESIHYGAVAVVDAQGRLVAHA
                     GDPDLVTYLRSSAKPFQLLPLVESGGADRFGFNDKELAVMAASHSGEPRHLEAVRGIL
                     EKIGLDESALLCGTHWPLSEGSARMLREAGREPAPIYNNCSGKHAGMLAQCAARGLST
                     HDYLDRRHPIQIAILDTLAALCDVDSAAIGVGIDGCSAPCFAIPLRACALAFARLADP
                     SDLPEPRRSAIQRVVRAMMAYPEMVGGEGRLDTDLMRAAPGRLVCKGGAEGYHGIGVV
                     KGGLGIAIKIVDGNGQRAGGPAAIEALRQMGILDGDEVESLRKYHRPAVRNHRGLEVG
                     EVRPVFELVYG"
     gene            complement(29564..30898)
                     /locus_tag="AC162G_06695"
     CDS             complement(29564..30898)
                     /locus_tag="AC162G_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075535.1"
                     /translation="MMTASGATVLEDFQSYTSVNGRIYLPDLRNNDSGWTSEFYVRND
                     GTESRTVTIYYFNANGTSTPKVSDTCILNPNQRCWIQVSAFDRIPAGTTGSAYIDGGE
                     AVSVVVIQRRASPGARAAYLGVSQPSTQVHVPLLHKNNYGLYSEIYIQNNWPTFTGVT
                     VQYKVSPGQPGSDCTESSNVLARGLKVIRLSEVSCIGATFVGSAYVTSNANHALAVAS
                     TQRKSDHSNMAESSGAGGTANTAYAPLIQNNNYGWISGIALQNASGSTNNLAANVYNQ
                     TGGWPPCRTDTYSNIGARRSQIISTPGVFCTSVASAVYGGGSQPVAAIVNQVLPGTPS
                     STDYEAITAPSKKVVVPLWLNRSSWISGLVVQNVNDQWANITITFYNDAGNQSGSPLT
                     AGIPPRALYIRSSTGVASGSAVVTADRPVAVVVNHFLSISGDGSMSHIGVQS"
     gene            complement(31013..31573)
                     /locus_tag="AC162G_06700"
                     /pseudo
     CDS             complement(31013..31573)
                     /locus_tag="AC162G_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
     gene            complement(31653..32411)
                     /locus_tag="AC162G_06705"
     CDS             complement(31653..32411)
                     /locus_tag="AC162G_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075536.1"
                     /translation="MSTQPNILKASLLLAAIAILTVLASCQPEGLVFDPQSGQWISPL
                     PTPTSPTSPLPTPTVASGPTPTPYPTPWPPDFTPEPIVLPTPGPLIGPQTLYDPISHF
                     SLTLAPGWYASVPDSHFASVTDIANYNFYTESRPLNGISIQIQAGPLPPSQSFEQWLA
                     DFRVRETSPESGAFGVTLTAPQPYKLGPYEGVAYVATGQKGESVLVLLMQSGDGWVAV
                     ISLSPYDASNPPSPALSEALSMLSTIQISSHPLR"
     gene            complement(32875..>33227)
                     /locus_tag="AC162G_06710"
     CDS             complement(32875..>33227)
                     /locus_tag="AC162G_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075537.1"
                     /translation="DEPPPGSVRMDITVSELDAGQSFEQWLADRRVLETSPEYGAGGV
                     TLTEPQPYTLGRYTGVTYTAYRSSSNDAVTIIYLLTSDGRIVGIGVWPVEALALSDVL
                     SMLSTLEVSPKPLP"
BASE COUNT         5964 a        10302 c        10804 g         6157 t
ORIGIN      
        1 actatcgtcg cgtcgtgcgg caggccgccc agacccagac acctggcctg ctacgtgcgg
       61 cgttcctcat cgaggatccc acgacctgtt acagcctgtc catatggacc agctgggacg
      121 ccatccctcg tttcgggacc aacgtccccc gccacgtgga ggctgccaat agggtctttg
      181 gacgggtgtg cttcagcaaa gaccgcggac ctgaaatttg gtcgaccaag tggcggttga
      241 cctcggtgag taacaacctg aattgggagg attttgacct gcgagcgttg atcctgggta
      301 tgggcccttg atcgaagggg gtatgtctcg ccagatggaa atctctgtgc ttcctcattt
      361 cgcctttgct tcccaactca gcttgggcat tgttttcctg ctctcggccc tgcccaaact
      421 gcgccggccg cttgccttcg tgcagagcgt agttgagtat aaggtcttac ctgccaaggt
      481 cgcctatgtg tttgccctgg cactgattcc gctggaagcc ttcctggcga ttgctttcct
      541 gacgggctgg tgggctgacg ccgctctgcc gctcgccacg gtgatgttga ttgtcttcct
      601 catggcggtc gggaccaacc tgaggcgggg gcggaagatc gattgtggtt gctttgggaa
      661 tacaagcgag cagatctcac cccgcacaat ggcccgcctg ctgctgctcc tgactactgt
      721 cctgcttctg gtggtcttca aaggcacagg gggcgcatca ttgccaagtc gcggagcgat
      781 gactgctgat gtatcaacgt tcatctactt actgcaaacg gcgtttctgg ccgcgtttct
      841 catcctgctg gccgcctgga tgctgagcct gcccgaggtg gtccctttag ttcgccgttg
      901 gcgctggagc caatcttcgt ctggtaacac gaaaaccgga gacggggtgg agggtgcata
      961 atatggatat gtcgatcccc ttttacctct cctatgccgc tctatggata ctcgtgatct
     1021 tgcacagcct gatcttgctg ggcgtggtgc gtatcgtata tcagctccag caaaccagcg
     1081 tggccgccgg cagccgtggt ttgagttcgg gcaaagaagc gccgcagttc agcgctgtag
     1141 ctctctccgg tgagccgatc agtagtacgg actttgtagg tcgcctgaca gctctcttgt
     1201 ttgtctcccc cagttgtccc tcatgcacag caatccttga agatgacatg ggatacctga
     1261 accacaaagc acagggaaat atgatcgtga tctgccgggc tgggcgcgaa gactgtgccc
     1321 gactcgctga gcagcatagg ctcagcatgc ccgtagttgc agacgaggac gaccagatca
     1381 gcggcctcta tcgtatttct accgttccca cagctgtcct catcaactcc aacaatcgta
     1441 tccaatccta cggtcaaccg cagcgcgagg aattggagga gatgctcgaa aaagccgcag
     1501 aagtgggggc tcagggagta ggttaacatt atggcagaaa gtgcggtcga agctgggcgc
     1561 ctggcttcga gccaggaaga tgtgactaga aagttgaggc gtatggcgct ggccattcca
     1621 gccatcgccc tgattgtggg tgcggcactt acagcttttg agccgcggtt cgggctcttc
     1681 ggggctgcgc tcatcctcgg ctggacacaa ctggttgggc tctgagggca gtcgctcgtt
     1741 ggagcgttag cgcctctcgg gaaggtggcc ggatacaaaa agaagtggtt gggagaggta
     1801 atcgcctaca ccctggctgg cagcgtggca agtgcatcgg tcggtgcggt ggtggggtgg
     1861 ttaggtggac tgctctggcc tggacaggtt agcaggctcg ggatcctggt ggccctcgcc
     1921 gtcgggttga tcgcgatcgc tcgtgaggtt ggatggctct cctttcccct gcccgaattg
     1981 agaaggcaga cggaaggtgt ttgggccaag ctgttctcgg gcacagtggc tgcgacgctc
     2041 tggggatttg accttgggct ggtttttaca gcccggctca cgttctccgg tgtctggctg
     2101 ctcgttgccg tcgccatgct cgtgggagag ccggcgttcg ggtcagcgct gttcgtcctg
     2161 tactggcttg gccgtgcgct gtcggtttgg atcgctccgt tactgatgcc ggatgcggac
     2221 gctaccccgt gggttctgga tgtgatatac cggcactacc ggctgtttca acggatccac
     2281 gtgttggggc tcgtctggtc cacggttgtt ctcatagtat ggctcagtaa aggaacgtaa
     2341 ggctgacttt cttgcggcag gtataggacc tgccacgcta gcagctgtgc actacaggag
     2401 gtgtgaaatg tccactgaaa agctggtcga aaccctcggg aagcatatca ggcgcagggc
     2461 attccttaaa agcctgggag ccagcacttt cgccacgctg ctggcgctgc tggggcaccc
     2521 ccccgtgagt gcatctcact ttctccctga ggattgccag aacggtacca gccaatacaa
     2581 atgttgctgg ttgtgttgca cccccaccgg gccctgctcg ggtggctgtc ctgatcaata
     2641 cacccacggg cagtggtgct ggtcatgcta ctttgccgat gacaaccatg actaccggtg
     2701 ctgtgaatgc aagaacccta acacgatctg cgcgcaaaac tgcagcgggg tctacaagtc
     2761 atattattac cgattgggga gtgcacctga gcctgccggg tgagattcgc tcgctgcgtt
     2821 gacagggcac ttcacgcatc gttgtgcgag gccggggcag gatcaccgcc gcaatgactc
     2881 agcagccacg atatgccagt ggggttgccg acacgcccat gtgcactgct cttactgccg
     2941 gtggtactgg cgggacgcac gggcattgat aggaaaggaa cttctgtgaa ggaacctcta
     3001 catgccgaga cgtgtattac tggagaacat tatgcgacga ggaggtacgt agccttggcc
     3061 cagcttaagt gcctgttagg tttgtctttg ttggcagcgg cgttagcggc ttgtaccggc
     3121 gccagtgggt tggcttccga agcgaccacc ccagcgcggc catcacccca tgtgccaact
     3181 catactcccc gattgccgtc gtctgtgaac cctgcagaaa cgccgtcccc cttccccacg
     3241 ccagcaccgc tgacaaagga tgccatcgct tcctgtccag tgtctctgcc taatgtcggg
     3301 acgtcatcac caaacgagca ctatgtaaat cctatttccg gttacggaaa cggggcttta
     3361 tggaccaccc tcgagcctgg aggaaagatc gttgccagac tcgacccgac gaatgaagct
     3421 ggttcactaa attggaagat gggctggtat cgaggggttc gaggcgaact gaccgtcacg
     3481 ggtcgacggc tggacgcgcc cgcgccgcca gcccaggggc tttatgatag ttcagggtat
     3541 ggtgacatcg gatttcaagc cggtgcgatt cactttccga gtgaagggtg ttgggaaatc
     3601 accggcaggg tgggcgacgc cagcctgacg tttgtgaccc tggtggtgaa ggttcccttt
     3661 gccgctgcat ggccacactg gggaccggag ggattggtca ttaaagacca ggacgttacc
     3721 ggtctaccga agacgatccg gctcatcttt ggcactccca cttggggcga aggcggggtc
     3781 acttggggcg aaggcgaggt cagcatcgag accacgcagg gtttatggga caaccccgac
     3841 ccctacccgg atgctgctac acagcaagtg acggtcaatg gacagccagg agtgtgtgtg
     3901 cagggcgcct gggatgcgca aggtcaatgg cgagatgagg cggacgcggg cgccctggag
     3961 tgggccgccg agggcttcag ttatcagatc agtcacattg ggctaggact gcgctgtgag
     4021 gacctgctgc gcattgcagg atcaccgccc tgatactaca gtgcgctgcg aagcatcgct
     4081 tagcaatggc attatcgggt actttgttcg gcggaggtct cctgaccccg ccaggttccc
     4141 gaccgtatgt ctcaattgcc ttgatacagt ctgcgctgag acctccggtc ggcttgagga
     4201 gcgggtcgga gacgccgctc ccatctcggc gctggtcgcc tgtcaccgga acggcagtcg
     4261 aacggtgaac gtgctgcctc tgccgggctg gctctcgacg tcgatcgtgc cgtcgtggtg
     4321 ctgcacgacg gcgtgcacga gcggcagccc gatgcccatg ccgccgaaca ggtgatcgcc
     4381 cttctcatcg cggagatgag accactcgaa gatgtgaccg atgacgtccg gctccatgcc
     4441 gacgccggga tcggtcacca cgatgacggc gtgccgcccc tcgcgcctca ccttgacgac
     4501 gatgtcgccg ccgtcgggac tgaacttgat cgcgttgtcg agcagcgcgt tgatcgcctg
     4561 cgacaacgtg gattcgtcgc cgcgcaagcg cggcagcccg gtctcgatct cggcgaggat
     4621 gttcagcccg gattccccgg ccctggacgt ctgccgctcg acgatgcctc gcacgatgct
     4681 ttccaggttg atcagctcca tctgcgggac gacgagctcg atctcctcca ggatcaagat
     4741 gtcgttgacc agttgcacga tccggtcgac gttgcgagtc aacgtctcga cggcgtggtc
     4801 gaagtcggcg tctttcagct tgccagcctt aagcaggtgc gtatagcccc tcaccgaggt
     4861 caacggcgtg cgcagctcgt gcgagatggt cgtcaggaat ccgctgcggt gcttgatttc
     4921 gcggcgcagg tcgtcgtaag cctctctcag ctccgcgttc ttccggcgca gctcggcgat
     4981 cgcgcgctga tcggcgtcgc gcagccgtgc agcgaccgcg cgcatgatgg ccgcggccat
     5041 cgccggattg ctatcgatgt actggctgaa atcctctttg gaaatttcca gcagctcggt
     5101 atcctcgatc gccgtcgccg tcgccactcg aggaacgccc tgcaccagcg ccagctcgcc
     5161 gaagaactcg cccgcgccct cgcgtgcgag gatgcgcgag gtttcgccgt cgagccgctt
     5221 gctgatctcg actgttcccg actcgatgat gaaaaacgtg tcgccgtaat ccccttcacg
     5281 gcagatcacg gtgccggccg ggtacgcgcg ccagtgcgcc gtgctggcca tgtacgagag
     5341 ccacgagtcg gtcaacccgg gaaatacttg ccggaggaat tcggctcggt cttttgcgct
     5401 tgtcacgtgc cgctccatgt ccccctgcac gcgattgtag catactcgtc gcggcggtcg
     5461 tgttgcggtt gagttacagg atcgacgcct ccgcgccgca attcgagcaa taagcggcgc
     5521 gggggcgcag gcgcgccccg catcgagtgc acgcgcgaga cgccgcaagg tcaatagagc
     5581 gaccgcactg gctgcagaac tgcgccgtgg atggcatctg cgccccacag gtcgtgcagt
     5641 cgaccgtatc ttcgtcggcg acaggccggc cctgtttgcg aagcgccgga cgccgtctaa
     5701 gcgcgagcca ggagataaca gccagtgcgc ccgcgcccgc tgcgcagacc gccgcgatcg
     5761 cgccggttgg gctgccggcc ggcgcgacgc ccgagggcgc attttccacg gtcgccggct
     5821 cggctttcgt cacgccgcgc ggaggcgccg gcagtcgcag cacgcccgat ccggtcgcgt
     5881 cgggaccggc gtcgctcttc accggtatcg tgttggcgag gagcgagacg cggaggtcgt
     5941 cggcgtcaaa gccaggatgg gcgctccaaa tcaaggccgc tgcgcctgct acgtgcggcg
     6001 ctgcggcaga ggtgccgaag aacgcttccg gcgcgtaggt cgccaccgat acaccgtcag
     6061 gcccgagcat ttccggtttc tggcgaccgt ccccggtagg cccatacgag ctgtacggtt
     6121 gcagcgtgtt gtcgcgaaag tcaacggcgc cgacggtgag tgcgccgcgc gagtcggcgg
     6181 gcgtggccaa gctgtgtgag ggcgtcgtga attccatgct tcgcgcttcg tggatataca
     6241 ggtcgagccg cgcgggccgc gacgtgccgt ttgcctgcac gctcacgtag tacgcgcccg
     6301 cgtcgtcaaa ccgatacagg atttgctcca ccggcgcgcg gttgcccgcc tgaatgttgc
     6361 gcgaggaggc cagtacgacc ccgtcgcgat ccagcaaata caggtcgtaa tcttcatcgg
     6421 cttgcggcca gtcgtcccag ttgagaacga tttgcgtcgt ttgatcgggc acgggacgaa
     6481 acgccagcag tggactttcc ggcttgaaat cgtggaaccc gtcgccgtcg gcatcgctat
     6541 acgtggtgcg gagatgctcg tctgcactgt tgccggccga gttgatccat agcacgtcct
     6601 gcgctgtgac gcggtcgacc agctcggcgt cgcgccccgt tccatccatt gggccagcca
     6661 gccctccggc cgagtgcgaa atgatctgaa cgccctgttc cagcagccag tcgaccgcgt
     6721 tgcccatgct tacgtccccg ccgtcgtagt gtgcgaagaa caactctgcg cgcggcgcga
     6781 catcgtggac gatctcggcc acggccgtgc cgtgattctc gcccgtcgcg tcaggctcga
     6841 cgccgctcac gaaggagcga gcgatcacgt gttcgggcaa ctcgcgcccg agctgatcgc
     6901 gaaagccgtc gaagccctga tcgagcacgc cgatctggat gccttgaccg gtgaagccgg
     6961 ccgcatgcca ttccgccgcg ttgatcgcga acacgccttc gctcacgatg gaacggctcg
     7021 acgactgctg cacacgtggt gagcggcgcg cgctctgcgg cacgctcggc gggttgagcc
     7081 gcagtccgac gacgtgctcg acgtcgcgaa tctgctcgaa gatcgctccc gggttctccg
     7141 actgtgccac accggcgatc acattcagcg ggatgctaat gtcgatcagg tctcgataga
     7201 tgccgaggac gttgacgccc agcgcttcca gatcgtcgat caggcgtgcc gtgtccgtcg
     7261 tatcgacgac gagcgtgacg cgcacctcgt tgtccggcgt cagcagccct cgccggcgcg
     7321 ccagctcctc ggcggcgaca agcccgccct gctcgtaggc gagcacgaac tccttgtaga
     7381 cggaatcggt ctcgggatcg cgcagcaggc gttcgaggct cgggtagcgc agcgccaact
     7441 cgtcgagcga gggcggcgtg atcgtcacgc gcggcgcggg tggaaacgtc agcgtgggcg
     7501 ccgggacgga gctgcccgat ggccgggaga acacgagcca cgcggcgagg ataaagacga
     7561 cggcgaaaac gacgacgaaa cggagccggg cttgtggcat gcgagactat cgcgatcgaa
     7621 acgtgagtat gagctggccg cacctcaacc gatcgccgtc ccgcagcaga gcttcctcgc
     7681 cggcggcgat gcggcgcccg ttaaggaacg tgccgttcgt gctgcccgca tcctccacca
     7741 cccacacgcc gccccgctgg tacagccgcg cgtgcaggcg cgacaccccc tcgcccggcg
     7801 tgccgtgcgg cgccaaatcg atatccggat agatctcatt acgcttgtcg gcgcggccga
     7861 tcgtcaattc ggctttcggc ggcaggagga tggtgctgcc ggaggccagg acgaaatggg
     7921 cgccctggcc gcgctgcgac tcggcttccg cgcgttcgag gctgggcagc ggcgtcgtcg
     7981 gcgcgcgcac gccggcacgc agcgtcgctc cgcacttagg gcagaaacgg cccagtgtgt
     8041 tgacgctcgc gccacagcgg gagcacgtac cggagcgcgc gatcatcggc gtgtacgtgg
     8101 cgtcgactga gccggctgcg gtctgcgtca gcgcaacctg cagcgcgttg accacctctt
     8161 ccgtcgattg gtagcggctg ccggggttct tcgccatcgc gcgcaggatg atcgcctcac
     8221 ccatcgccgg aaggctgggg ttgagcgcgc acggcgattc aggcgtcacg ttgacgtgct
     8281 gcatcagcac gtccagcgcc gtgtcgcccc tgaacggccg cctgccggtg acaagctggt
     8341 agagcaccac gcccagcgag tagatatcgc tgcgcacgtc gacttccaca gcctgcgcca
     8401 gctcgggcga cgcatagtcc ggcgagatga tcgaatcttc gtctgccgat aacgaccggc
     8461 gcacgtcggc gattctcgca atctggaagt cgccgagcag gggccaatcg tcccgcgcca
     8521 ggaggatgtt ggccggtttg atattctggt gcgtcacgcc ctggctgtgg gcgtatgcca
     8581 gcgcgcgcgc gacgggcaga atgagcgcgg ccgcagcgct ccactccata ggctggccgg
     8641 tcaggtgcga ttccagcgcg ccgccgtccg cgtaatccat ggccagaaat acgacgcctg
     8701 cgcgctcgcc gaactcgtag acactcaaga tgtgagggtg ccgcaattgg gcgacggtgc
     8761 gcgctccgcg cttgaagcgc gccaggaacg ccggctccga ggcatgcttg ggatcgatca
     8821 ccttgagggc aacccagcga tcgaggccct cctcgtaggc ccgatacacc gtggcgaggc
     8881 ggccctgacc gattttttcc gtaattcgat attggcccag ttccttgccg acgaggtcca
     8941 tttggacaac ggatcttgcg aattgacgga tttctaaccc ggtcgattcc gaacgatgct
     9001 tctatccgtg taatccgtgc gaatccgagt gtgcgctacg tttcgacccg cacgacgatg
     9061 gcgctgatgt tgtcgacgcc tccggcggca ttggccgcgt cgatgagcgc tcgggcggcg
     9121 tccattgacg tcgccgcgcc gcgtatcgtc gaggcgagga ccgcgtcctc gcgcaccatc
     9181 tcccacagcc cgtcggagca cagcagcagc gtgtcgccgg ggtgtaactg ttctacgaac
     9241 agatcgatct cgacttccgc ttcgcgcccg agcgagcgga cgatcacgtt gcgcaccgga
     9301 tgcgtatacg cgtcctccgg tttcacgttg cccgacgcga cgaggccggc gatcagagag
     9361 tggtcgcgcg tgatctgacg catctcgccg ccgcgccgca ggtacgtgcg gctgtcgccc
     9421 acgttagcga taatcgccac atcgccatat accagcgtca tcgtcaatgt ggttccggcg
     9481 ttgccggctt ctgcgggttg agcctgagcc tgcgcacgca ccgtccggtt agcggcctgc
     9541 accgcgcgca agacacgctg ccgcagcgag tcgcggtcgg ccgggtgggt cgcgccgaac
     9601 cgcaacggcg ccaggacggg cgtgcggtcg atgccagccg gctcggtctg gtctggtccg
     9661 ggaggggaca gcaaatcgcg cagtgactcg cgcgcggacg cgacggccca gtggctggcc
     9721 agttcgccgg ccaactggcc gccgacgcca tccgccacga cgaacaggcc caccggttca
     9781 tctcgcgcgt tgcgcgtcac gagataaaag atcgcgtctt cgttgtgctc gcgttccaga
     9841 ccgacgtcgg tcagcccggc ggcgttcagc cttaatctca tcgtcaactg tccatgcgtc
     9901 tctgcgccag cacgctcagc ccgccgaaaa gcaggcagaa ggcgaccagc acgccccagc
     9961 gctcgatgac gtagctcggc gaatgcccgt agtttacatt aaaatcgacg cgcaggcgat
    10021 ctcgcaccgt gaccgggcgc tccaccgttt cgttggtcac gggatcgacg ctctcgaccg
    10081 tcttggtcac ttcgattcgc ccgaggtcat tgaggcccgg cagatcgacc gacgagccga
    10141 gggcttccac cgtccagcgt gtgacggtga ggtacgaaag cccctgcgtc gcgcccggca
    10201 atgggaagat cacgccggcg aacatgatct gcgcgaacac ggcgaacagc acgacgtaga
    10261 tcgccgtcgc agcattgttg acgatcgccg agatgaacaa cccgatcatc gtgccggccg
    10321 cagcggacag cgccagggtc aggtagatct ccagagggcc gggcaggaat gtgcccgcct
    10381 gcggaaacgg cacgcggatg ccgaaggcga cgatcagcgc caggcactgg atcagcgcga
    10441 acccgacgag aacggctgct ttcgatccga gatagggcgc gagccgcagg ttgaccattc
    10501 gctcgcgccg gtaaatcgcc cgctcgcgca ccagctcgaa cgacgcgacg aacatgccga
    10561 gcagcgcggc ggccagcccc agcatcatca acaccgactg cgcctgcgcg gcgatggagt
    10621 agcggccagt ctcgtgcaag atgtccgcca cgcgctcctc gctcgcgccg acgagcgctt
    10681 ccggatcggc cagcagtgac aggagcacgc cgatgatggg catcaccgcc agcaggaccg
    10741 ccagccgcac actatcgcgc aagaccagct cgaacgtgcg ttgggtgagg atgaagaact
    10801 ggcgcaagaa actgacgcgc ggccgggccg gaagcgaaat gtccgtggct gagcgcggcg
    10861 cttccggcag gtcgctgagt cgcgcgtcca agacctgctg cgcggtggtc gaggcccggt
    10921 atcgctcttc ccacagctcc gggttccgct cgatttcggc gtaaatgtcc gcaaagtcgg
    10981 cggcgccgaa gaagtcgagc gcggcggccg gcgggccgaa gaacaccagc cgcccaccgc
    11041 tcaaaaaggc gacgtggtcg cactggcgaa tattggccgt cgcgtgcgtc acgagcacga
    11101 tcgtccggcc ggcgtcggcc atgcggcgca gagtatacat gagtttcttc tcgaggccgg
    11161 gatccagccc cgaggtcgct tcgtccagaa agaataggct cggctcggcg atcagttccg
    11221 cgccgatcga gacgcgcttg cgctggccgc cgctcaggct cgacacgatc tgctcgcgat
    11281 gatcggccat ttcgacttcg gccagcacgc gccggatgcg ctgctcgatc tcgtcggctg
    11341 acgtgtccga gggcaggcgc agttgcgcca cgtagcgcag cgcgcgatcg acgggcaggt
    11401 ccaggtgaat gatgtcgtct tgcgggacgt agccgagcag gccgcggtac agcgcgtagt
    11461 gcgcgtaaaa atcgtcgccg ttgagcagca ctcgcccttc ggcgcggcgg acaccgctca
    11521 gcgcgtcgag cagggtcgtc ttgccggcgc ccgatccgcc gacgatggcg acgaactcgc
    11581 gcggcaaaat cgacagcgag acgtcccgca ggatcaggcg cacgcccccc tcctccctcc
    11641 ccccgtcaga acggggggag ccggaggggg gcgcgcgccg cgtgaggttc agcgcgtcga
    11701 tgcgcgcctg catggctccg acgacctggc tcagcgtcga gggttggtag gtcaggcgga
    11761 acgggccaat gtgcaccacg tcgcccgggc gcagcgtcac gccgctattg ggctggagac
    11821 ggctgccgtt cacgtacgtg ccgttggtgc tggacaggtc gatgagcacg tggccggccg
    11881 gcacgcgctc gatgcgcgcg tgccggcgcg atacgaccgg cgaatcgaga tgaagatcgt
    11941 tcgccgggtc gcgccctatc gtcaggctgt cggcctgcgc caggtcgagc tgccccaatg
    12001 atacggattc gacggcgtgc gcggcctgat acgtcaggct caccgagttg cccagcgcgt
    12061 cgccgatgcg gatgatgtcg ccgtcgcgca gcgtgaccgt cgaagcgcgc cggccgttga
    12121 cgaacgtgcc gttgcggctg cctcgatcga cgacgcggtg tccacggcgg ccgtcgatca
    12181 cgtgctcctc gatcagcgcg tgctcggatg ataccgtcgg gatcggcacg acgatatcgt
    12241 tggtcggatg gcgcccgatg gtgaaggtct tcttgcggag gggcatggtc tgcgccgcgc
    12301 catccggcca gcggatcgcc aggtgcgcgg cggcgaccgg catgagctgc gtggagccag
    12361 cggacggagg ggaatctcgg gaaacagtca tgggcgtctt acgttttacg ctttacgttt
    12421 caggcctgcc tgaagatcag tcgcaggctg ccgagtaaga tatgatcgcc gtctgcgagt
    12481 acaatctttc ggccaggctc caggcgctcg tcattgacgt aggtgaggtt cacgctattg
    12541 agatcctcga gcgtgtagcc gttttcggcg cggcgcaggc gggcgtgttg gcgcgaaaca
    12601 ccgtaggcgt cggcgccgta gggcgcaagg tcgacgtccg gcctcgcccc gctcagcaca
    12661 tcctcgcgcc cgatcgtgtt ttcggctcga tcgggcacgg cgatgcgcca accggtgtcc
    12721 tcgtggatca gcacggcacc tcccggcaag gtcgcgccgc atgcgtcgca atagcgatcg
    12781 ccgggcaatg ccgtcacgcc gcagcgggtg caagtcactc ccggcgcgtc gcgcaggcgc
    12841 gatccacacg cgtggcagaa gcgcgcggcc tgcacgttca atgtgccgca ggcggggcag
    12901 taacgatcgt tcacggtcgt caagcgactg gagagaccgg cgaccagaga ttagcgttcg
    12961 gaatttgacg gttgccggat ctcgatctcg gcctgctctt cgggtttacc cagcacgtcg
    13021 atctggatca gaataacagt gatattgtcc aggccgccgg cccggttggc tgcgtcgatc
    13081 aggcgctggc atgcctcctg tggcgaaggc gcggcaagaa tgatctggtg gatgtcgtcg
    13141 tcctcgacgt actggcataa cccgtctgag cagagcagca cacgatcgcc cggagcgagc
    13201 gcaatgcgct tgacgtccgg ctcgatcttg gaccggtcgc cgatcatctt gtagatgacg
    13261 ttgcggttct cgtgcgtgcg cgcttcttcg cgcgtgatct ggcccgtatc gaccagacgc
    13321 tgcacgagcg aatgatcgac ggtcagttgc gtgatgtcgt ctcccctgat gagataagcg
    13381 cggctgtcgc cgacgtgcgc gatatgcgcc tcgccgtcga ggatcacggc catcaccagc
    13441 acggtgccca tgtcggtgcg cgcaacggcg cgctggcggt gcaccgcttc gttggcggcc
    13501 tggatcgtgc tgcgcagcca ggatggcacg tcgacgtgga gcggcccttc gtcgatgctc
    13561 tgcgccagga gaccttcgag cgcctggcgg gcgatgaccc gaatggcaat gccgctggcc
    13621 acgtcgccgg ccgcgtggcc gcctgcaccg tcggcgacga cgtagacgcc gaccggacgg
    13681 ctgaccgatc gatgcacttg cccgacgtcc agggtgagca gcgagtcctc gttaagctgc
    13741 cggtggtggc cgacatcggt caggcggccg acgcgcagat cgtacgtcgt gggccggcgc
    13801 acttcggcca gcgcgctgcg caatcgatcc accagcgacg cggcgctgac gagctgcgcg
    13861 ttgaacacgt cgcggaacaa ttgtgtcagg ctgggcggtt cgagcaccac gcgcggcgaa
    13921 taaatctgcc cggtgagcag cacgaacagc aaggccgcca gcgaggtgac gtcttctcgg
    13981 aacagcttgc gcgcctgaac cccttcggaa acgatgcgcg ccgaggtgaa atcaaaccag
    14041 cgcgccatcc tgttgtcgag cgagacgtgc gatagatcgg cggcgccgaa cgcgatgtta
    14101 cggccatgca ggaaagccag gctctcggag agcatcatcc cccactgcag gatgtcgagc
    14161 agctcctgcg gggcggccaa tgatgccccg gtcagcggcg acggttgggg cagaacgacg
    14221 tagtagcgcc gggtgccggc gatcgattcg gagaatgtat cgacgggcag gagcgcgccg
    14281 ccgtgataca ggcgcatgtc ggccagccgt cgctcgatgg cgatcgcgtc ggcggcgatg
    14341 ctctctttga cgatgaactg cggatggtgt ggctcgatgc cgacgaggag cgcaccgcac
    14401 tgctggcaat attcatcgcc gaacgaattg ccgctgtacg cgcacgaggg gcaccggacg
    14461 cgcttgtgcc ggtcttgcgc ggcgtatgtg ttcaccgacc catcttcgct gatcacgctc
    14521 agtatttcgt agggaagcgc gccggccagg gcgccgacgg gcagcgcttc gaagaaatag
    14581 cgggggcgcg gcaggggctg cgtgtcgtcg atgccggccg gagatacggc aggcggcgcc
    14641 tgctcgtcgc cgtcaagcgg tcgtgtgatc gtgcgcgagt caaacgggcg ggtgcccagc
    14701 gtgttttcgt cgatcggcgg cgcggtcgcc acatcgccgg cgccatccgc cgcgctggat
    14761 tcgggctgag cctcgcgcgg cagcgccgtg ctgcaccggc ggcagaatcg cgcctcgggt
    14821 ggattatgcg tgccgcacac gacgcagttc atcgggacat cctcgacgca gtgggcgatt
    14881 cggacatccg gaatacaaac tcgacgctcc cgaacgaaac ctgataaccg tcccgcagta
    14941 gattgcggcg tgtagccagg ccattgaccc ggatgccgtt cgtcgagtct acgtcctcca
    15001 caatgtagtc cacgtcttcg cgccggatct gcgcgtggcg ccgcgacacg gtgagcgcgc
    15061 cgggcacatc gggagcgatg cgaatgtcgt tgtcggcgcc acgcccgatg cccatcaacg
    15121 gccggtgaag ctcgtagatg cgcggacctt catcttcgcc gaggcgttcg agaaaagcga
    15181 tggcgactgg ctcgcgtggc tgcgccagtt ccgctgccag ttgagccttc cgccgcctga
    15241 tgcgggacgc gacgacgaat cccgcgacga gcacccccag cagccccagc gtaacgacga
    15301 tcgcgagcaa gggatctagc gtcatgtgta ttcccagagt aacatctata gattatagcc
    15361 ataaatcggc tcgatcggaa attgcccatg cgtggggaaa gggtggataa tcaccagccg
    15421 accggccatt gacgagtgcg cacgtccgag ctaaactaac gcgcgtgccc gcccgtctct
    15481 ctcgacactt ttttgcccgc ccgaccctgc gcgtggcgcg tgagctgttg ggctgcaggc
    15541 tggttcgccg aatcgacgga cagcgcttgt ccggcgtcat cgtcgaggcc gaagcgtacg
    15601 tcggggaaaa cgacctggcc tgccacggcc gccgtggacg cacgccccgc aacgcggtca
    15661 tgttcggcaa gccaggcgtg gcttacgtat acttcaccta cggcatgcac tggatgttca
    15721 acatcgtcac ggagcgcgaa gggttcgcgg cggccatcct ggtccgggcc ttggagccga
    15781 tcgaaggcat cgcacggatg cgggagcgcc gcaagcaggc tatcctgcga aacctctgca
    15841 gcggcccggc caaattgacg caggcactgc gcatcgaccg gcaggataac ggtgttgatc
    15901 tatgcgcacg cggaaccgat atctggatcg aggcggggga gccgctcgcg gcgaaacaaa
    15961 tcggccgcag cccgcgcatc ggcgtcggtt tcgcgcccga accgtggcgc agcaagccgt
    16021 ggcgcttttt cgtgcggggc aacccgtttg tgtcgagcta accaaccact ggagggtgac
    16081 cgatgggact gctggatggc aagaaggcgc tgattttcgg agtggccaac aaggactcca
    16141 tcgcgtgggg aatcgcccgc atgtttcacg agcacggcgc gacgatcggc ttctcgtcga
    16201 tcgccgatct cgtggagcgg cgggtgcgcc cgctggccga gtcgatcggg tcgacgttcg
    16261 tcgagccgtg cgacgtgcag agcgacgagc agatcgcggc agtgttcgag aagtggaagg
    16321 cggcctacgg cgatctggac atcctggtgc acgcgctggc cttcgccggt cgcgaagcgc
    16381 tcgccggccc gtatctcgac gtcacgcggg ccgggttcca gttggcgctc gatatcagtg
    16441 cgtattcgtt gacggcgctg gtcaaagccg cgcacgatgt gctgcgcgcc ggatcgagcg
    16501 tcatcacgct gacctactat ggctcggaga aggtcgtccc gcactacaac gtgatgggcg
    16561 tggcgaaggc cgcgctggaa gcaagtgtgc gctacctggc cgccgatctc ggcccgcgcg
    16621 gcgtgcgcgt caacgcgatc agcgccggcc cgattcgcac gctgtccgcg aaaggcgtct
    16681 ccggtttcac cgacatgtac aagcacttca aagacatgac gccgctgcgg cagaacatca
    16741 cggccgaaga tgtcggcaaa acggcggtgt ggctcgccag cgatctggcc cacaacgtga
    16801 cgggagcggt cattttcgtc gacggtggga tgaatgtgct agggctggcc gtgccgccgg
    16861 tgacgaggga cgagtaatcg gtgatcagta attagtaatc agtgatcagt ggtcagtcat
    16921 cagtgatggg agttagagtg ttgagtgaga gcgttgaatc gacggaagag agtgtcagct
    16981 cggctccgcc cagcccgccg cagtggaaca gccagcgcgc cccgccgacc aggaatcggt
    17041 cgagcagcat ctgcaagcct tcgcggttgt cccgcgtgtc ggatacgacg ccgattttca
    17101 tgctacggac gaggttcccc ctgaagccct tgccacatct gtggagaata taacgaactt
    17161 cagatactgg tcgtatggaa cgatcacggc ctcgggccgg ctgccccgcg tgaggatata
    17221 gggagtcggc ccgatattct ttatcgcgca cgatgagcca ccttcgcctg ccagcagtcg
    17281 tggcgatgta acagtagcga ggcctgggtg gacaggccat ggaagaaagc cgccgtggcg
    17341 gatgaaaaga agcggccaat ggttggacgc gctaggaccc cgtcaagcac tcgagtgcgc
    17401 caacttgcgg attcaagcga tagaagtatc gcgatggtgt cggttacact gttggcacac
    17461 gcagcccatc gaggagtgca cctatgaagg tcaagatgat cctgcccgcg ctaaccgaag
    17521 ccaaaagccc ctactggcgg ccgatcaaat actcgctctt cccgccgctg ggactcgcca
    17581 ccctggcagg ctacctgtcc gaagacgacg aggtcacaat tcaggacgag cacgtcgaaa
    17641 cgctccacct ggacgacgag cctgacctgg tcgtgatcca ggcctacatc acgtccgcct
    17701 accgggctta cgaactggcc gactattacc ggcgcagggg cgcacacgtc acgctgggtg
    17761 gactgcacgt cacttcgctg ccgggggaag ccgcgcggca cgccgacacg atcttcctcg
    17821 gccctggcga agacacctgg ccggctttcc tcgccgactt ccgcgccggt cgaccgcaga
    17881 agctgtaccg ttcgcaaatc cgcacgctga tcggcctgcc gcacattcgc cgcgatctga
    17941 tcaagcgtca tctgtacctg gtgcccaact cgatcgtcgt gtcgcgcggc tgcccacaca
    18001 cctgcgactt ctgctacaaa gaggcctttt tcagaggcgg caagtcattc tatacgcagg
    18061 ccgtcgatga cgcgctggcc gaaatcgagc gtctgcctgg ccgacacctg tacttcctcg
    18121 acgatcacct gttcggcaat ccgcgctttg cctcggcgct gttcgatgga atgcagggga
    18181 tggggcggct ttggcaggca gcggggacag taaaatcggt gttggctccc ggcctgctgg
    18241 agaaagccgc cgcctgcggg ctgcgcagcc tgttcgtcgg ttttgagacg ctgaacccga
    18301 tgaatctgcg cgagcagcgc aagtatcaga acctcaatca cgactacact gccgcgatcc
    18361 ggcgcctgca cggtctgggc gtgatggtga acgcgagctt cgtgttcggc atggacgatg
    18421 acgacgagtc ggtgttcgac cacacggtcg actgggcgat cgagcagggc atcgagactg
    18481 cgacgtttca catcctgacg ccatatccag gcacggcctt gtatcagcgc atgatgcagg
    18541 aaagacgcat cctacatcac aattgggatt tgtacgacac gcggcacgtc gtcttccggc
    18601 cggccaaaat gtcggcagag gtgctggaag cgggttactg gcacacctat cgtgagttct
    18661 atcgctgggg atcgatcttc cgaagcgcat gggccaagga cgcctggtcc gaacggctgc
    18721 gccacatggt ctacgctggt ggatggaaga agatggagtt tgtgtggcac gggattattc
    18781 gactgaagcg agtatctgcg atgctgcctg tattagaggc gactctggga ggtttcaggc
    18841 gtctatcgga gcgcgacgcc cggcagcgcg ctggcgtgtc cggcgctcat gtttgactca
    18901 tcagcacaat ccgcgttcat ctgcgtccca aacgcctcac actcaactcg actgaagctc
    18961 gtccttcacg gtgcggtaga gcgactcggc cttgaggtcc ctgagcgagt agcatggcgc
    19021 gtcgaggtgc tcggcaagcc gggcggcgag accttggtcg aacgcctgat gctccatgtt
    19081 gatcaccacc gcgcggatgt ccagctccgc gatctgctcg gcgacgttgt acgcctcgac
    19141 ctgcggcgag gcgctgccct ggctgacgtt gcccgcgccg tcggtcagca cgatcaccag
    19201 cggcgcgacg tcggggtgga cctgcatctc gcgggccagc acgcgctgcg ccagcgtcaa
    19261 cccggcgctg agcggcgtct tccctccgac cggaatgtct ttcagcgcgc gctgcgccag
    19321 gtcgatcgat gacgtgggcg gcagcaccag ccgcgcctgg tctttctgga agacgatcag
    19381 cccgacgcgg tctcggcgct ggtaggcgtc ggtgagcaac gacatgatcg cgcctttcgt
    19441 ggcggccatg cgctcggcga cggccatcga ccacgacgcg tcgacgacga acaggatcag
    19501 gttggcggcc ttgcgaacgc ggactttctt ctggtaatcg ccgcgcccga cgcgcagcgc
    19561 cgggccttcg atcttctgcc gccggcgctc gggctggtgc ggcgcggcgg cgcgcaaggt
    19621 ggcgtcgaac gcgaggtcct tggggtcgcc gggtgagggt cgagccttga tgtatcgccc
    19681 gcgcttgcgc tcggtcttcg acgagctgcg ccggccggcg tgcgtgcggg tgatcttatc
    19741 gagcggagta tcgagcttgc ggacttcgaa ggtctcgccg atcggcacag cgtcaccgcc
    19801 ttcccaccag taggcgtctt gccggccgat cgtctgcgcc tgcgtgcgcg cttcgctgtg
    19861 cccgaagtcg gcggagtcct taacctcgcc ttcgggcgct accgtttttt tttgacgctg
    19921 ccttcctcgc cctcttcgtc gagcatcgcc tcttccccgc cggagaactc gctctgcgct
    19981 ttctcgagcc gctcgctcag gtcttgcatc gaaacctggc cgttctcctg gaagggctgc
    20041 cgcttcaggc gatgcggcag cgccagctcg gcggcgagga ggatgtcttc tctcgtgatg
    20101 tgatcgcggc ccattttcgc ggcgtgtgcg cgcgcacctt tcaggatgac gaggtcggcg
    20161 cgatggccgt cgacgcccag gctgccgacc atgccggcga tggccgccag gtcggcctgc
    20221 gtatacctga ccatgggcag cgtctggcgc gcgcgatcga tttcctcgga gagcaggtgc
    20281 tcgcgcggct cccactcttc gcggaaccct tcggggtcct gctcgaaggc caggttgcgc
    20341 tcgaggatct gcatgcgctg ctgctgatcg tggatgccgc ggatgtcgac ggcgagggcg
    20401 aagcgatcga gcaactgcgg gcgcagatcg ccttcttcgg gattcatcgt gccgaccagg
    20461 atgaagcggg ccgggtggct gaacgaaatg ccttcgcgct cgacgacgtt gacgcccatc
    20521 gccgccgagt cgagcagcag gtcgacgacg tgatcgtcga gcaggttgac ctcgtcgacg
    20581 tagagcaggc cgcgattagc agcggccagc acgcccggct cgaagcggcg aatgcctttc
    20641 tgaatcgcct gctcgatgtc cagcgtgccg acgacgcggt cttcggtcgc gctgaccggc
    20701 agatcgacga accgggtgcg gcgggtggtc tgcggcagtt ctgcgccctt ggcggcacgc
    20761 tcgcggcact cggtgcacca ctctgctggc gagcctggat cgcagccgaa gcggcagttc
    20821 tcgacgacgc caatctccgg caggagcgcg gcgagagcgc gggcggcggt cgacttggcc
    20881 gtgccgcgct cgccgcgaat cagcacgccg ccgatctgcg gatagatggc gttcaagatc
    20941 agggcgcgtt tcatgcgctc ctggccaacg atggcagtga agggaaagac gatgcgcatt
    21001 cgagctccgg ctcacctctc cagccccaag tgggcggggg agatcattgc tattcagatt
    21061 ataccttact cactcgtgaa cgggaagaat cctcagcgat ggagctaccg ggtgaaccag
    21121 ctctccatct gcccaaaatc gacgcgcgcg gtgaaatcga gcgtcagggg cgtgatcgac
    21181 accactttgt cgatcacgac ggctcgcacg tccgagccgg cttccagcgt cgcttcgtcg
    21241 atgcgcatct cgtaacccat tgggccggga tcggtcgggc gacggcgatg cgggatggtc
    21301 gggtagaaat agcgctggcg gctgatgctc gtccagcgat gcggcgtatc cggcatcgca
    21361 tcctggggta cttcgatctt gagcagatcg acgcccgccg gcaatcccca tctcagcaca
    21421 gcctcagaga acatccgcac gtagcgcgcc gccgccgaga agtcgatgtc ggcggagtga
    21481 ctgagatgga gcgcggcttc cgtctgccgc gaaacggcca gcgccggtat gccgaacgaa
    21541 gcgccttcca tcgccgcgcc gaccgtgccg gacacggtga tgctctcgcc gaggttttcg
    21601 ccgtagttga tgcccgatat aaccaggctg acgggtcgct gtgcgatttc gaacagcgcg
    21661 tgctcgacga cctgcgcggg cgagccttcg atgccataag cgacgatcgt gcggccgttg
    21721 accacgacgt cttccgggta aacgcggccc tcgctcgccg gcggcatcga gcggcctgcg
    21781 ccggactgct gcatggccgg cgcggcgatc agaagctctc ccaacggctc acacgccgca
    21841 gccgcagcgc gcagccccct cgactggatg ccgtcgtcgt tggtgatcag gatgagcggt
    21901 cgatccgtca tgtgtcattc ctcgttctgc acgcgacgga atcataccac aaacgtgata
    21961 cgtgatgcgt gaaacgtgat acgatacgcg gtaggcgata cgcgatacgc gaactgcacc
    22021 ttgacatttg cgcgcgagcg tcttatgctg tacgtgactt ccacacaagg agtatccatt
    22081 gccccgccat ccttacatcg ccgtcgaggg cgtcatcggt gtcggcaaga ccacgctggt
    22141 ccggctgctc agcaaagact tcaacgccga gcctctgctg gaagtcttcg aagagaaccc
    22201 gtttctctcc gacttctaca gcgatcgcgc gcgctatgcg ttccagacgc agatcttctt
    22261 tctgctcagc cggtatcgtc agcagcacga cgtcatcccg cgcgcgctga tgcacagcgc
    22321 gctgattagc gactacacct ttgccaagga cgcgctgttc gcgcgcctca atctgggcgg
    22381 cgacgagatt gccgtgtaca accggctgca cgccgcgctg gcagagaaga tccccaagcc
    22441 cgacctgttg gtttacctgc gcgccggtct cgacacgctg atggcgcgca tcgccgcgcg
    22501 agaccggcca tacgagcgcg ccatggatcg ccgctacatc gaaagcctgc gcctggcgta
    22561 cgacggcttt gtcgccgcgt acacggactg cccggtgttg gcgatcgaca cggacgagct
    22621 caatttcgtg caggatctcg gcgcgctcga ctccgtccgc gagcgcattc gcgccgcgct
    22681 ccggcaaggg acgtttcagc aggccttgcc ccatatgccc gtcacgctca ccgaatccga
    22741 tcgctcggcg ctcagcgagc tgcagcgggc gcaggcgcag gctggcgaga ctcgccgcga
    22801 tgcgatcgcc gagttcctgt cgctgacgcg gagcgtcggc gaagtgagcg ctgtgctgct
    22861 cgacggcggg gatcaaagga tgctgcgcca ttcgctggcg gcgtgcctga gccgcctcat
    22921 gcacctggcg gacgcgaccg gcgtggacct ggacgaagcg tatcgcgctt attctgcacg
    22981 caaatgaaaa ttccgacccg ctctcggcgc tgcccgaagg gcgtgccaaa tccgctgctt
    23041 gatttgacaa tccagcgcga gcgttcactc ggaaacttga aatgcgctcg gtatacgaac
    23101 tggatgggag ggcacggtga ctaaacactt cgtcgcgatc gcgggaaacg tcggcgcagg
    23161 aaagtcgagc ctcgtcggat tgcttgccga caagctcggc tggacgccgt tttacgaggc
    23221 cgtggacgag aatccttacc tctcagattt ctatcgcgac atgcgcacat ggtcgttcca
    23281 ctcgcaggtt tttttcctct ccaagcggct gcgccaccac cggcgcctgc tcgatcatcc
    23341 ctcgtcggtg ctgcaagacc ggagcgtgta cgaagacgcc gagatcttcg ccaagaacct
    23401 gtaccgccag ggcaacatgg acgagcggga ttacggcgtg tatcgcgagc tgtacgaagt
    23461 gctcacgttg tacctgccgc cgcccgactt ggtcgtgtat ttgcaggcga gcgtgccgac
    23521 gctcctgaag cgcatcgcac agcggggccg cgagtacgag cgcggcatct cggcggacta
    23581 cctcgccagc ctgaacgagc tgtacgaaga ttggatcgcc ggattcacgc tgtgcccggt
    23641 attgagcgtg ccctccgacg atctggactt cgtcaaccac gatgctcatc tcgctctggt
    23701 cgcgtcgatg atcaaggatc gcctgtccag tgcgccggtc gtttcgatgg tctgaagaag
    23761 tgcgcgtctt tcccgctgtg atataatccc aagaatgtcc ggccgagaag acaccggcgc
    23821 gacgtttatt gttgcgccct gcacgatcgg acgctcgggc cggcggcgct gccgggccat
    23881 ggtgcaatgc tcgcgaacga tagacatacc agctacctgg atcacctgac ctgctcacgc
    23941 tgcggcgcaa tctgcgcacc cgactcgctt cagaccattt gcccgacatg cggaagacca
    24001 ctcctggccc gatacaacct ggacgccgcg cgaaccaaac tggaccgtga gcggatggcc
    24061 gcgcgccccg atcgcatgtg gaactggcgc gagctgctgc cggtgcgcga cccgcagcac
    24121 atcatctacc tcggtgaagg tggcacgccg atgtttcacg cccgccggct cggcgagacg
    24181 ctgggcctgc ctcatttatg gatcaaggat gagggtctca acccgaccgg ctctttcaag
    24241 gcgcgcggca tggcggccgc cgtttcgcgc gcgatcgagc tgggcgcgcg caagttcgtc
    24301 gtgccgacgg cgggcaacgc cggcgcggcg gcagccgcct acggcgcgcg cggcggcgcc
    24361 cccgttcacg tctatatgcc tcaggacgcg ccccttgcga atcgagccgg cgcacaggca
    24421 gccggcgctg aagtgatcct ggtcgatggc ctgatcaacg acgccggcag gatggcagcc
    24481 acccacgcga aagagcgagg ctggttcgac ctttcgacga cgcgcgagcc gtaccgtgtc
    24541 gagggtaaaa agacgatggg gtacgagatc gcgcaccagc ttggctggca ggcgccggac
    24601 gtcgtcctct atcccaccgg cggcggcacc ggcctgatcg gcatttggaa agcatttgaa
    24661 gaaatgcagg cgctgggctg gatcgacgcg cgccgcccac gcatggtcgc cgtgcaggcc
    24721 gaaggctgcg cgccggtgca tcgcgcactt caagccggtg cggatcgctg cgaggcgtgg
    24781 cccgccgcgc atacggacgc cgccgggctg cgcgtgcccc aaccgtttgc cgacgagatg
    24841 atcctcgacg tgctgcgccg aagcggcggc gcgagcgtcg tcgtgagcga cggcgcgatg
    24901 cgggacgcgc aggagtgcat ccgccgccgg gagggcatct cggcctgcct ggaaggcgcg
    24961 gcgacggtcg ccgctttgcg ggcgctgctc aagacaggct tgcttcaggc cgacgaacag
    25021 attgtgtgcc tcaacaccgg aacgaacctt cacacggcgc tgccgccgcc gaaggacgga
    25081 gtcgatggct gataccgatg ccgatctcgc tctcgcccgc tcggcgggcg ccagatccgg
    25141 tgcggagaac gccggggcgg ccgcggccga ttcggaaagc ctcgcggcgc gcgtcgccga
    25201 gctagaacgc gcctatgaga gcctcatcga ggacagcgtc gttccgatcc tgattaccga
    25261 ttacgatggg aaagtgctgc gcgccaacgc ggctgccgcc cggctcgccg gtgtggcgaa
    25321 ggaagcgctt gtcggcgagc cgctcaacag cagccagttg tcgatcgagc tgcgtgtcct
    25381 gcaaacagcc ttgcctgccg cccggcagaa tcccgatctg ggcgagagac tcgagacacg
    25441 gttggccagc cgcaatctca gcctcgacct gcatactcgc cacatggtgt acgatggaca
    25501 gccggcgatc cgatggctcg tccacgatcg aaccgcgctg caggagctgg agcgcgtccg
    25561 cgacgaacag gtttacatga tcgtccacga cgtgcgtaac ccgctgggca acatcatcag
    25621 cagcctggag ctgttgaagg actcgctgca agatccgacg acgacgcccg cgccgggcgc
    25681 gctggtgaac atcgccctgc gctcgtcacg gcgcatttct ctgctcgtcg aatcgttgat
    25741 ggacatgacg cgcatggaag ccggacagtt cacgttgaac acgacgacgg cgcgcctcga
    25801 cacgctgatc gagcacgccg tcgagttcgt gaagcccacg gccgatcgaa agcgcatcgc
    25861 gctcaggacg gagatcgagc cgaacctgcc gccggtgctg atcaacgtca acatgatcga
    25921 gcgcgtcgtg gtgaacctgc tcgacaatgc atgcaagttc gtgacaccgg gccaggccgt
    25981 gaccatcagc gcgcggctcg gcaatggggc cgacgtccac atttccgtga acgacgaagg
    26041 gccaggcatc ccgccggagg atcagcgccg catcttcgaa aaattcagcc ggggcgcggg
    26101 catcggcggg caaacgcccg gcacgggcct gggcctggct ttctgcaagc tggccgtcga
    26161 gacgcacggc gggcgcatct gggtcgagag cgaactcggc aagggcagca cctttacgtt
    26221 tacgctacct atcgaagcca gttagaacat gaccgaccgc ctctattact ccgaccctac
    26281 ccttcgatcg ttcagcgctc gcgtcgtcgg gcggcttgag atcgacaacc acccggccgt
    26341 gatcctcgat cgcacggcgt tctaccccga gggcggcggc cagcctggcg accgggggca
    26401 gatcaatcag gctcgggcga tcgacgtgca ggcgcaggac gacgcggcgc acagcgtcgt
    26461 gcacattctg tccgagccgg tcgcggaaga cgaagtgacg tgcgcgatcg actggacgcg
    26521 ccgattcgac ttgatgcagc agcataccgg ccagcacatt ctgtcgcaag cgttcgtgcg
    26581 cctgctcgac gccgagacag tcgcctttca cctcaacgac gacccgaacg aaggcacagc
    26641 cacgatcgat ctggaccgcg ccggcttgcg gccggccgac gtcgacgccg tcgaggacct
    26701 ggcgaaccag atcgtgttcg aaaaccggcc ggtcacggcc cgcttcgtga gccgggaaga
    26761 gctggcatcg atcccgctgc gcaagccgcc cacggtcgac gcggcgatcc gcatcgtcga
    26821 gatcgcggag ttcgaccggt cggcgtgcgg cgggacgcac gtagcgcgca cgggcgaagt
    26881 cgggcagatc aagatcgtca agctcgagcg tcgcggacca gagacgcgcg tcgagtttcg
    26941 ctgcgggcag cgggcgctcg ccgactatcg gcgcaagaac gatgtgatca accgcgccgc
    27001 cgccggcttg tcaatcgggt tctgggagct ggaggaggcg gcggcgcgac tggtcgccga
    27061 aaacaaggcg ttgttcaagc agttggaaga ggctcaagag cgcctgctcg agttcgaagc
    27121 gcacgaactg tggtccaacc tgagagagca cggccagctt gcagccgcca tgcgcgtgtg
    27181 gcacgctcgt gacatgaacg cgctgcgcaa gctcgcgaaa caggtcatct cacggccgcg
    27241 cacggtggcg ctgctcggat cgggcgggga gaagccggcg ctcgtcttcg cgcgctcgag
    27301 cgatgtggcg ctcgacatga gcaagttggt gcgagacgcg gcggcgcgca tcggcggcag
    27361 gggcggcgga tcgccggact tcgcgcaggc aggcggcccg ccggcaagcg acgagcaggt
    27421 ctgggccgcc gtcgaatggg cgtatgagca actttcgtaa tggaaaagat cgccaataat
    27481 atctatgtct cctcatctta tcgcggcgtc accgtcggcg cgatcctcac gccgatgggc
    27541 gtggtgtgcg tcgacgcgcc gatgctgccc gccggcgcgc gtgactggcg ggcgaagatc
    27601 gcgtcagtca ccggcgcgcc gattcgctac gtaatcaata ccgacgcgca tcgcgaccgc
    27661 gtgctcggcc agcagtggct ggggggcgcc gtcgtcgcga ccgaagccgc cgccgataag
    27721 atgcgcacgt acggcgacac cttccggcag caggtggccg atttcttgtc gcatcatggc
    27781 gcacccgagg ctgccgagga aatcgcgctc agcctcaaga tggttcagcc tcaaatcagt
    27841 tttggcggca ggctggtgct acacgcgacc acaccgcgca tcgagatctg gaacgtcggc
    27901 ggcgcgaatc ccgccagcgc gtgggtggtc ctgccggacg ccggcgttgt cttcaccggc
    27961 gatctgctga cgctcaacgt gcatcccgtg atgtccgaat cgagcacacg cgtgtggctg
    28021 gagcggctgg ccgggctgcg caagccgtcg tttccggcca agaagatcgt gcccggacgc
    28081 ggtccggtct gccgcaaggc cgacacgcag gtgatgtcgg cttatctgcg cgacgtacgc
    28141 agcaaagtgc gcgcactcat caaggcgcgc aggcagaaac cggacgtggc ctcgctcgtg
    28201 gcggccttcc tgccgcgctt ccccatccca aacggcgagc gcgagcgcat gcagcggcgc
    28261 atccgcgccg gcctggagca cgtgtacgac gaactgaagc aggaaaaatg agcatcgcag
    28321 aatgtagaat gatgaatgta gaatgatgaa tgatgagtga tgaaaggcag atcagcaggt
    28381 atccgacgcc tttggtcgaa gtcactcgcg gcggcctcgt cgaatcgatt cattacggcg
    28441 cggtcgccgt cgtcgatgca cagggccggc tggtggcgca cgccggcgat cccgacctgg
    28501 tcacctacct tcgttcgagc gccaagccct ttcaactgct gccgctggtc gagtcgggcg
    28561 gcgcggatcg cttcgggttc aatgacaagg aactcgcggt tatggccgcc tcgcactccg
    28621 gcgagccgcg ccacctcgaa gcagttcgag gcatcctcga aaagatcggc ctcgacgaga
    28681 gcgcactcct atgcgggacg cattggcctt tgagtgaagg gtcggcacgc atgctgcgcg
    28741 aggccgggcg cgagccggcg ccgatctaca acaactgctc cggcaaacac gccggcatgc
    28801 tggcgcagtg cgccgcgcgc gggctgagca cgcacgatta tctcgaccgg cgccacccga
    28861 tccagatcgc gattctcgac acgctcgccg cgctgtgcga tgtcgattcc gccgcgatcg
    28921 gcgtagggat cgacggatgc agcgcgccgt gctttgccat tccgctgcgc gcctgcgcac
    28981 tggcgttcgc ccgcctggcc gatccatccg acctgccgga gccgcgtcgc agcgccatcc
    29041 agcgcgtcgt gcgggcgatg atggcctatc ccgagatggt cggcggcgag gggcggctcg
    29101 acacggattt gatgcgcgcg gcgccggggc gcctggtgtg caaggggggc gcggaaggat
    29161 accacggcat cggcgtcgtg aagggcgggc tggggattgc cataaagatc gtcgacggca
    29221 acggccaacg agccggcggg ccggctgcga tcgaggcgct gcgccagatg ggcattctcg
    29281 acggcgacga ggtcgagtcg ctgcggaagt atcaccgccc ggcggtgagg aaccatcgcg
    29341 ggctggaggt gggcgaagtg cggcccgtgt ttgagctggt ctacggatga tgaaaactgg
    29401 gcactgataa ctgaccactg accagtaatc agtgagcaat gagcagggtc aggccctcct
    29461 tgagtgccgg ggatctccct gtccaagaat gagttagcgg ttcatcccca tgtcgatccg
    29521 gacattctgc cgcgcatgcg cgccgaaggg cgctacgcgc tgtctaactt tggaccccga
    29581 tgtgactcat gcttccatcg ccactgatgc tcaggaagtg attgaccacc acggccaccg
    29641 gccggtcggc agtcaccacc gcggaaccgc tggcgacgcc ggtgctgctc ctgatgtaca
    29701 gcgcgcgagg cggtatgcct gccgtcaagg gcgaccccga ctggttgccg gcgtcattgt
    29761 agaaggtaat cgtaatattc gcccactggt cgttgacgtt ttgcacgaca agcccgctga
    29821 tccagctgga tcggttgagc cacagtggaa cgacgacctt tttactggga gcagtgatcg
    29881 cctcgtagtc cgtcgacgaa ggcgtgcccg gcaatacctg gttcacgatg gcggcgaccg
    29941 gctgactgcc gccgccgtag accgccgagg ctaccgaggt gcagaagacg cccggcgtcg
    30001 agatgatttg cgatctccgc gcgccgatgt tggaataggt gtcggtgcgg caggggggcc
    30061 agccgccggt ctggttgtag acgttggcgg ccaggttgtt ggtgctgccg ctggcgtttt
    30121 gcagggcgat gcccgagatc cagccatagt tgttattttg gatgagaggc gcgtaagccg
    30181 tgttggctgt gccgcccgcg ccgctcgact ccgccatatt gctgtggtcg gacttgcgtt
    30241 gggtggaagc caccgccagc gcatggttcg cgttactggt gacgtaggcg gagccgacaa
    30301 aggtggcgcc aatgcagctg acctcgctga gtcggatcac cttgagtcct cgggccagaa
    30361 cattgctgga ttccgtgcaa tcactaccgg gttggccggg cgagactttg tattgcaccg
    30421 tgacgccggt gaatgtgggc cagttgtttt gtatgtaaat ctcgctatac agcccatagt
    30481 tgttcttatg cagaagcggc acgtggacct gggtactggg ctggcttacg ccgagatagg
    30541 ctgcccgggc tccgggggag gcgcgccttt gtatcaccac caccgacacc gcctccccgc
    30601 cgtcgatgta ggctgaaccg gtcgtgccag cgggaatacg gtcgaatgca ctcacctgaa
    30661 tccagcaccg ttgattcggg tttaggatgc atgtatcaga gaccttcgga gtggacgtgc
    30721 cgttcgcgtt gaagtagtaa atcgtgacgg tacgggactc tgtcccgtcg ttgcgcacgt
    30781 agaactccga ggtccagcca ctgtcgttat tgcgcaggtc gggcaggtaa atccgtccgt
    30841 tcacactggt gtaagattgg aagtcctcaa ggacggtagc gcctgacgcg gtcatcatcg
    30901 ggctgggccg gtgtccctgc ggcgcaaggg gtgtttctcc attcggacaa gtggtgctag
    30961 gtgggatagc cgtattgcaa tgagagaagt aaccgttgtt atcatagtgc cgaaaggtta
    31021 aatgcagatg cgtagcattt ggatcggtca ggccgcttgc cccaacctta ccgattaaag
    31081 tccaagtgtg aactgagtcc gtttcggcta ccggtacttg tgataaatgc gcgtacaatg
    31141 tccgatagcc gttcgcgtga tctatttgca cgtaacatcc ataactgggc ggaggggaac
    31201 aattgggagg tgccgcaaca tcggaaacaa atccatccgc tactggcagc accgctgcac
    31261 cgttgtcatt cgtccgattc caatccgtgg cgtagtagtc gttattagga tcgttacaat
    31321 ggtcaacatt cccataaaac gagccagttc ctcccactgt ccacgtctca gtctcacccg
    31381 caataaacgg catctgtaac gttatcgtgc tgtcataggc ttcggtgccg gggtaggtgg
    31441 gaccatcggg gccggggcaa gccaacgcca tttgtagagg cctggaaacc aggatgccca
    31501 gattggcaag cactgctacc ataactgcac gtagtgcttt gtcaattggt ctgttatgag
    31561 aattgtggcc catgttatgc ttcctcaatg tcggtcttta ccaaatggct tgcttgcgaa
    31621 atgcccagtt acatttcagt catttgatga gcctaccgaa gcggatgtga tgatatttgg
    31681 atggtcgaca acatggatag ggcctcagag agcgcaggtg agggcggatt cgatgcatca
    31741 taaggactca aactgatgac tgcgacccac ccatcacccg attgcataag aagcaccagc
    31801 acgctttcgc ccttttgacc agttgcgacg taagcgactc cttcgtaagg cccgagtttg
    31861 taaggttgcg gtgctgtcag cgttacacca aacgcgccac tttctggaga tgtctccctg
    31921 actctaaaat cagccagcca ttgctcaaaa gactgactag gcggtagtgg gccagcttga
    31981 atctgaatgc tgatgccatt aagcggacgg ctttcggtat agaaattgta attggctatg
    32041 tccgtaacgc tggcgaagtg tgaatctggc acgctggcat accagccagg ggccagcgtc
    32101 aggctaaagt gacttatggg atcatagagg gtttgcgggc ctattagtgg gccaggtgtc
    32161 ggtagtacga taggttcggg cgtgaaatcc ggaggccagg gggtgggata gggcgtgggt
    32221 gttggcccgc tggccacagt cggtgttggc agtggagagg tcggcgaggt aggtgtcggc
    32281 agcggcgata tccactgtcc gctctgtgga tcgaatacca gtccctctgg ctggcaacta
    32341 gccaggactg tgagtatggc aatagccgcc agcagcagcg aggctttgag aatattcggt
    32401 tgagtgctca tcgctaggct ccatcctgag atacgagcga acttgcgctc actacgcaac
    32461 ggctgatgaa gcgcacagca tggcgtcaga gcggcagtcg ttctcgtacg aaaccattat
    32521 acataaaacg gggggggggg tgtcaagatc caaatgagcc atctccccca ctgtccacgt
    32581 ctcacccgca atgaatggca tctgcaacgt tatcgtgctg gcataggctt cggtgccggg
    32641 gtaggtggta ccatcggggc cggggcaagc caacacggct tgaagaagca ttgcatcata
    32701 cacacccaga atgacaagcg ctgccaccag ggccgtgcgt agcgatttgc taaatgatcg
    32761 gttacgagga ctgtgacgca tcttctgctt cctccacgcc aagtttagac ccacggcttg
    32821 gctttcaaaa ggcgtcaatt gccaggctgt ttttcggcat tcactgaggc tatgctatgg
    32881 gagtggtttg ggtgaaactt ccagggtgga taacatagac agcacatctg agagcgccag
    32941 ggcctcgaca ggccataccc cgatacccac gattcgccca tcgctcgtga gcaggtaaat
    33001 tattgtaaca gcgtcgttag aagatgaacg atatgctgtg taagtaacac ccgtgtagcg
    33061 cccgagtgta taaggctgag gttcggtgag ggtgacacca cctgcgccat attccggaga
    33121 tgtctcaagg acccgccgat ccgctagcca ttgctcaaaa gactgtccag catcaagttc
    33181 gctgacagta atatccattc tcacgctgcc gggtggcggc tcgtcca
//
LOCUS       DBNHXB010000169         6939 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2890989, whole genome shotgun sequence.
ACCESSION   DBNHXB010000169 DBNHXB010000000
VERSION     DBNHXB010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6939)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2890989"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..167)
                     /locus_tag="AC162G_06715"
     CDS             complement(27..167)
                     /locus_tag="AC162G_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075538.1"
                     /translation="MAIPIPATLRAAVVERAGERCEYCHFSRWALSSETRRVFLPAVL
                     KN"
     gene            complement(167..517)
                     /locus_tag="AC162G_06720"
     CDS             complement(167..517)
                     /locus_tag="AC162G_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006623482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075539.1"
                     /translation="MVEITLEVPESLAERLEAVRSRLPEVLAHGLDELSPLPNKTYRY
                     ILEFLVSQPSPEAVLTFKPTSEMQARVEELLEKNRAGQLSPAESAELDEYVRVDHLIT
                     MLKAHTLPYLPAAS"
     gene            complement(585..3401)
                     /gene="polA"
                     /locus_tag="AC162G_06725"
     CDS             complement(585..3401)
                     /gene="polA"
                     /locus_tag="AC162G_06725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR5075540.1"
                     /translation="MPKKKLILIDGHSLAYRAFYGLPLYDQRGKVAFSTSRGEYTNAV
                     YGFANTLIKVWNEEKPDYIAVAFDLGRTFRDDKYAAYKGTRDKMPDELTPQIDFIVRL
                     VEAFDIPAITAEGYEADDILGTLAKRAAADGLDVLIVTGDTDAFQLIGKRVRVLTSGR
                     VYSDTATYDEAAIKERYGLKPAQLIDFKALKGDTSDNVPGVKGIGEKGATDLLRKYGS
                     VESIYKNLAKVEPARARNALEAGRDMAFLSKDLVTIRTDVPIRVAWEDCAVHTYDRGK
                     VEALFDYLEFRTIRNRLPEGGVAPDRAPLAGAQMSMFGDEPVTEARARPIVKQKPSVT
                     KTMIVQDEAALQSLISNLQKANIISFDTETTGTDPLRSSLVGIALAVKEGEGYYIPVG
                     HTPLTNNQLPLQKVIQSLKPLLENSNLPKVAHNAKYDMAVLRQAGVDVQGVVFDTMLA
                     EFLIDPSARLGLKQLAKARLGVEMTEISELIGTGKKQITMAGVAIEDAAPYAAADVDM
                     TLRLMKAQEPELKKLGVRKLFDEVEMPLVPVLLDMEMAGVLIDVKLLKKMSDRLAKRL
                     AAIEKEVYTSVGMVFNINSPQQLADALFGKLQLKAPGGRQTATGKVSVAADVLESMRG
                     MHGAIDLILEHRQLSKIKGTYLDALPELVHPKTGRVHTSFNQTGAVSGRIASQDPNLQ
                     NIPIRTELGREVRKGFVVPAGWKLIAADYSQVELRIVAHICKDPGLRTAFASGLDIHR
                     ATAATVLGIPPEKITADQRSFAKRVNFGLLYGMGTRTLAEQAGIRMAEAQKFVDAYFA
                     GFPNIKKYIDETKRRAKEEGYVETLLGRRRYFPILQTQTRDARTNIMQRTAEREAINH
                     PVQGSAADIIKMAMIRIHASLRQKKLKARLLLQVHDELVLEAPDNEVQKVSKLVREIM
                     ESAYPLDPPLKVEIGVGENWDEVK"
     gene            complement(3704..4531)
                     /locus_tag="AC162G_06730"
     CDS             complement(3704..4531)
                     /locus_tag="AC162G_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5075541.1"
                     /translation="MPTRVLILDSNEAFAMLLKEALEADREFRAVDVSSSHSALNALQ
                     SDEFDLAIVDLDLHDMEPAALLRAIRELRPDLPIVVVPIDGDVIPQELAPFDIKGMLT
                     KPFFLPELPARVADALGRPLPASAPMPAAAPASAAWPQNSSRALPRIKLSPGDPRVSD
                     ALHALVAALNAETVLLTVGDALLAHAGPLGPSGSATLAHRILDSRAVSARSSWIAPSH
                     EQVRFGQSVSDSGEHMLYSLDVAEGVLLTVAVRPESPLRLVRSKTRQAADALIALSQ"
     gene            complement(4654..5673)
                     /locus_tag="AC162G_06735"
     CDS             complement(4654..5673)
                     /locus_tag="AC162G_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075542.1"
                     /translation="MPKPNTGVQGQFIDPNGATYVAWAADLGVGWIKQQVDWNLIESA
                     PGQYGWDDLDQFINQAESHGFKILLSVAKAPGFSRSEPVEEDGPPLDFAIFQRFTLAL
                     ASRYRGRVDAYELWNEPNLAREWRGHPLSAERFVELIRLGAAGIREGDPEAIIVSGAP
                     AVTGIDDKVNAIDDRVFLREMVAAGVAGWVDAIGAHPYGAANPPGERAADATHRASGY
                     NDHPSFFFLDTIEDYRAILLEAGVDKPIWITEFGWPSIEGYGEVETTGWEYAREVTEA
                     DQADYLRQAIRLRRDRPWLGPMFVWNLNVAWLLGPGRPDSAYGMVRPDGSLRPAYSRL
                     RNSGE"
     gene            complement(5982..6197)
                     /locus_tag="AC162G_06740"
     CDS             complement(5982..6197)
                     /locus_tag="AC162G_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR5075543.1"
                     /translation="MYRFLIVIEKANGNYSAYSPDLPGCAATGKTREQVTRNMYEAIE
                     MHIQGLRADKLPIPKSHSYAEYIAVQR"
     gene            complement(6200..6412)
                     /locus_tag="AC162G_06745"
     CDS             complement(6200..6412)
                     /locus_tag="AC162G_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HIR5075544.1"
                     /translation="MKVRDVIQIIESDGWYLVVTKGSHRQYRHPTKPGRVTIAGHPSH
                     DLAPGTLNSIFKQAQVEKPSRKKERK"
     gene            complement(6529..>6939)
                     /locus_tag="AC162G_06750"
     CDS             complement(6529..>6939)
                     /locus_tag="AC162G_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HIR5075545.1"
                     /translation="LAGQRPKTLVDPIPVETLRREIMATIRDWGQQILDKPETFNNRF
                     YQTFIVLNYCRMLHDLHTGNTGSKRAGAEWAKATLDPSWAGLIDRAWDGRPDPALSVR
                     QPADPKDFKSTLEFIQCIIEASTQYAAALENGRT"
BASE COUNT         1237 a         2072 c         2215 g         1415 t
ORIGIN      
        1 caccttttgg ttaatcagcc cgtccatcag ttcttcaaga ccgccggcag gaacactcgg
       61 cgcgtctcgc ttgacaacgc ccacctggag aagtggcagt attcgcaccg ttccccggcg
      121 cgctctacga cggcggcgcg aagcgtggcg gggatcggga tggccattac gaggccgccg
      181 ggaggtaagg cagcgtatgt gccttgagca tagtaatcag gtgatcgacc cgcacgtatt
      241 cgtcgagctc ggcggactcc gcgggcgaga gctggccggc ccgattcttt tcgagcagtt
      301 cttcgacgcg cgcttgcatc tcggacgtcg gcttgaacgt cagcacggct tccggcgaag
      361 gctggctgac caagaactcc agaatgtagc gatacgtctt gttcggtagc ggcgatagtt
      421 catctagtcc gtgcgccagc acttcgggca gccgtgaccg tactgcctcc aaccgctcgg
      481 ccagtgattc gggaacttcc aaggtaatct ccaccatgtc atctctccta acataaaaga
      541 tcgtagcaat aaccggatgc aatgtcaagc aggacacacg ggtcttactt cacctcgtcc
      601 caattctctc ctacccctat ctcaaccttc agaggcggat cgagcggata cgcgctctcc
      661 atgatctccc ttaccagttt ggagaccttc tgcacttcgt tgtccggcgc ttcgaggacc
      721 agctcgtcgt gcacttgcag gagcagccgc gccttgagct ttttctgcct gagtgaagcg
      781 tgaatgcgga tcatcgccat cttgatgatg tcggcagccg agccctggac ggggtgattg
      841 atcgcctcgc gctcggccgt gcgctgcatg atgttcgtgc gcgcgtcgcg cgtctgtgtc
      901 tgtaagatcg ggaagtagcg ccggcggccg agcagcgtct cgacgtagcc ttcctctttt
      961 gccctgcgct tcgtctcgtc gatgtacttc ttgatgttgg ggaagccggc gaagtaggcg
     1021 tcgacgaact tctgcgcctc ggccatccga atgccggcct gctcggcgag tgtgcgcgtg
     1081 cccatgccgt agagtaaacc gaagttgacc cgcttggcaa acgagcgctg atctgccgtg
     1141 attttctccg gcgggatgcc gaggacggtc gccgccgttg cgcgatggat gtccagcccg
     1201 gacgcaaacg cggttcgcag ccccgggtct ttgcagatgt gcgcgacaat gcgcagctcg
     1261 acctgcgagt agtcggcggc gatcagcttc cagcccgccg gcacgacgaa gcccttgcgc
     1321 acctcacggc ccagctcggt gcggatggga atgttctgta gatttgggtc ttgggatgct
     1381 atgcgcccgc tcaccgcccc ggtctggttg aacgaggtgt gcacgcggcc cgtcttcgga
     1441 tgaaccagct cgggcagcgc gtcgaggtac gtgcccttga tcttcgataa ctgccggtgc
     1501 tcgaggatca ggtcgatggc accgtgcatg ccgcgcatcg attcgagcac atcggcggcg
     1561 acggatactt tgccggttgc cgtctggcgc ccgccgggcg ctttgagctg cagcttgccg
     1621 aacagcgcgt cggcgagctg ctggggcgag ttgatgttga acaccatgcc gaccgaggtg
     1681 tacacctcct tctcgattgc cgccagccgc ttcgccaacc gatccgacat cttcttgagc
     1741 agcttcacgt cgatcagcac gccggccatt tccatgtcga ggaggacagg cacgagcggc
     1801 atttcgacct cgtcgaacag cttgcgcacg ccgagtttct tgagttcagg ctcctgcgcc
     1861 ttcatcaggc gcagcgtcat gtcgacgtcg gcggcggcgt acggcgcggc gtcctcgatc
     1921 gcgaccccgg ccatcgtgat ctgcttcttc ccggttccga tcagctcgct gatctcggtc
     1981 atctcgacgc cgagccgcgc cttggctaac tgcttcaagc ccagccgcgc gctggggtcg
     2041 atgagaaact cggccagcat cgtatcgaag acgacgccct gcacgtcgac gccggcctgg
     2101 cgcagcacgg ccatgtcgta cttcgcgttg tgggcgactt tcggtaaatt actgttctcc
     2161 aataacggct ttagggattg aatgactttc tggagaggta attggttatt cgtaagtggg
     2221 gtgtgcccga cgggtatgta gtagccctca ccttctttga cggcgagcgc gatgccgacg
     2281 agcgaggagc gcagaggatc ggtgccggtc gtctcggtat cgaacgagat gatattggct
     2341 ttttggagat tggagattag agattggagc gccgcttcgt cttgtacgat catcgtcttc
     2401 gtgacggacg gcttctgctt cacgatcggg cgcgcgcgcg cctccgtgac aggctcgtcg
     2461 ccgaacatcg acatttgcgc gccggcgagc ggcgctcgat ccggcgccac gccgccttca
     2521 ggcaggcggt tgcggatcgt tcggaattcg aggtaatcga acagcgcctc gaccttgccc
     2581 cggtcgtacg tgtggaccgc gcagtcctcc cacgccaccc ggatcggcac gtcggtgcgg
     2641 atggtgacca ggtctttgct gagaaaggcc atgtcgcgcc cggcttcaag cgcgttgcgc
     2701 gcgcgcgctg gctcgacctt ggcgagattc ttgtaaatgc tttcgaccga gccgtatttc
     2761 cggagcaggt cggtcgcgcc cttctcgccg atgcccttga cgccggggac gttgtcgctc
     2821 gtgtcgccct tgagcgcctt gaagtcgatc aactgggccg gcttgaggcc gtagcgctcc
     2881 ttgatcgccg cctcgtcgta tgtggccgtg tcgctgtaga cgcggcccga ggtcagcacg
     2941 cgcacgcgct ttccgatcag ttggaaggcg tcggtgtcgc cggtgacgat cagcacgtcg
     3001 agaccgtcgg ccgcggcgcg cttcgccagc gtgccgagga tgtcgtcggc ttcgtagccc
     3061 tcggcggtga tggccggaat gtcgaacgct tcgaccagcc gcacgatgaa gtcgatctgc
     3121 ggcgtcagct cgtccggcat cttgtcgcgc gtgcccttat aggccgcgta cttgtcgtcg
     3181 cgaaaggtgc ggcccaggtc gaaggcgacg gcgatgtaat cgggcttctc ctcgttccac
     3241 accttgatca gcgtgttggc gaagccgtag accgcgttcg tgtattcgcc gcgcgaggtg
     3301 gaaaaagcga ctttcccgcg ctggtcgtag agcggcaggc cgtagaaggc gcggtaggcc
     3361 agcgagtgac cgtctatcag gatgagtttc tttttaggca tggtgattcc ggtattgggt
     3421 tgattcgtaa ttagtaatta gtaaccagtg gccagtaatc agtaatcagt gagcagtaat
     3481 cagtgagcag taatcagtga gcagtaaccc gtgagcaccg ataactgatg actgacaact
     3541 gatgactgac aactgataac tgacgactga taactgacaa ctgataactg acaactgatg
     3601 actgacaact gatgactgac aactgatgac tgacaactga tgactgacaa ctgatgactg
     3661 acaactgata gctgacaact gacaactgat gactgatcac cgatcactga ctcagagcga
     3721 tcaacgcatc cgccgcctgc cgggtcttcg aacggacaag gcgcagcggc gactcgggcc
     3781 gcaccgcgac ggtcaggagc acaccctcgg cgacgtcgag ggaatagagc atgtgctcgc
     3841 cgctgtcgga gaccgactgg ccgaagcgca cctgctcgtg actcggcgcg atccacgacg
     3901 acctggccga gacggcgcgc gagtcgagga tgcggtgcgc cagcgtggcc gagccggacg
     3961 ggccgagcgg gccggcgtgg gcaagcagcg cgtcgcccac cgtcagcaag acggtctcgg
     4021 cgttcaacgc cgcgacgagc gcgtgcagcg catcgctcac gcgcgggtca cccggcgaga
     4081 gcttgatacg cggcagcgcg cgggacgaat tttggggcca ggcagccgat gcgggcgcgg
     4141 cggccggcat cggcgcggac gcgggcaggg gccggccgag cgcatcggcg acgcgggcag
     4201 gcagctcggg gagaaagaac ggcttggtca gcatcccctt gatgtcgaac ggcgccagct
     4261 cctgcgggat gacgtcgccg tcgataggaa cgaccacgat cggcagatca gggcgcagct
     4321 cgcggatcgc gcgcagcagc gcggccggct ccatgtcgtg aaggtccaga tcgacgatcg
     4381 ccaggtcgaa ttcatccgat tgcagcgcat tcagcgccga gtggctgctg gacacgtcga
     4441 cggcgcgaaa ctcgcggtcg gcctccaaag cctccttcaa cagcatggcg aaggcttcgt
     4501 tggagtcaag gatcaggaca cgtgtgggca taggcacatc tggccgcgat cgaatacgga
     4561 tccctgtata ccggattggt aactcgcaat gacgtgggct gagtagttac ctggattgta
     4621 ggattcacgg cagaaaatgt caacaccccc ttatcactcg ccggagttgc gcagtcgtga
     4681 gtacgccggg cgcagcgatc catcggggcg aaccatccca tatgccgagt ccggccgccc
     4741 cggtcccagc agccacgcca cgttgaggtt ccacacgaac atcggtccca gccacgggcg
     4801 gtcgcggcgc agccggatgg cctgccgcag atagtctgcc tgatcggctt cggtgacttc
     4861 gcgcgcgtac tcccatcctg ttgtttcgac ctcgccgtag ccttcgatcg acggccagcc
     4921 gaactccgtg atccagatcg gcttgtcgac gccggcttcg aggaggatgg cgcggtagtc
     4981 ctcgatcgtg tcgaggaaga agaagctggg atggtcgttg tagccgctcg ctcgatgcgt
     5041 cgcatcggcg gcgcgctcgc cgggcgggtt cgccgcgccg tacggatgcg cgccgatggc
     5101 gtcgacccag cccgccacgc cggcagcgac catctcccgc aaaaagacgc ggtcgtcgat
     5161 ggcgttcact ttgtcgtcga tgccggtgac ggccggcgcg ccgctcacga tgatggcctc
     5221 gggatcgccc tcgcgaatgc ctgccgcgcc cagccggatc agctcgacga atcgctcggc
     5281 gctcaatgga tggccgcgcc attcgcgcgc gaggttcggc tcgttccaca gctcgtacgc
     5341 gtcgacgcgc ccgcgatagc gggatgctaa ggcgagcgtg aaccgctgga agatggcgaa
     5401 atcgagcggc ggaccatctt cttcgaccgg ttccgatcga ctgaaacccg gcgctttcgc
     5461 cacgctgagc aggattttga atccgtgcga ctcggcctgg ttgatgaact ggtcgagatc
     5521 gtcccagccg tattggcccg gcgccgattc gatcaggttc cagtcaacct gctgtttgat
     5581 ccagccgacg cccaaatcgg cggcccaggc gacatacgtc gcgccattcg gatcgatgaa
     5641 ctggccctgc acaccggtgt tgggcttagg cacaggtgtc ggtgtaggtg tcggcgttag
     5701 tgtcggcgtt ggtgtcggcg atggggagga ggtagccgtc ggccggcctg ccggtcgctc
     5761 agtcggcggg gaggccggcg agacagccgg agatggggcg ggcgcggtgc acgaggcgat
     5821 cgtggctgtc gcgagcacag cacacaagat gattggacgc atgctgcgat gataacacgc
     5881 agcacacaag ccccccttga ttctggcatg ggggccaggg ttgggtggcc gattaagggt
     5941 tgggacccct cgtgcgcggg atgaaagcgg tagccaccgc tttatcgctg aacggcgatg
     6001 tactcggcgt aggagtggga cttgggaata ggcaacttat ccgcgcgcaa gccttggatg
     6061 tgcatctcga ttgcttcgta catattgcgt gtgacttgct cgcgggtctt gcccgtggcg
     6121 gcgcagccgg gtaaatcggg tgaataggcc gagtagttgc cgttggcctt ttcgattacg
     6181 ataaggaaac ggtacatgct tactttctct ccttcttgcg tgacggcttc tcaacctgtg
     6241 cttgtttgaa aatgctgttg agtgtgcctg gggccaagtc gtgactgggg tgcccggcta
     6301 ttgtaacacg tcctggtttc gtcggatgtc tgtattgccg atgactgcct ttcgttacca
     6361 ccagatacca gccgtcactc tcaatgattt ggattacgtc acggactttc atctgctgac
     6421 tccggccagc gaggtggagc gattgtagca cacgagccgc agatgttaaa gcggggtatt
     6481 atgactcttg ccaaactcct ccaggtggaa tgatcgaacg catatggatt acgttctacc
     6541 attttccagg gccgcagcat actgcgtact tgcttcgatg atgcactgga tgaactccag
     6601 agtgctcttg aagtctttgg ggtcggcggg ctgccggacc gagagagccg gatccggacg
     6661 cccatcccac gctcgatcga taagccctgc ccatgatgga tccagggtgg ccttggccca
     6721 ctcagccccg gcgcgcttgg atccggtatt cccggtgtga aggtcatgca gcatgcggca
     6781 gtagttcaag acgataaacg tttgatagaa ccggttgttg aaagtctccg gtttgtccag
     6841 gatctgttgc ccccagtcgc ggatggtcgc catgatctct cgacgcagcg tctcaacggg
     6901 tatcggatcg accagcgttt tcgggcgctg cccggccag
//
LOCUS       DBNHXB010000170        10042 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4568683, whole genome shotgun sequence.
ACCESSION   DBNHXB010000170 DBNHXB010000000
VERSION     DBNHXB010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10042)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10042)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10042
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4568683"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..2375)
                     /locus_tag="AC162G_06755"
     CDS             complement(111..2375)
                     /locus_tag="AC162G_06755"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR5075546.1"
                     /translation="MFTPRPSQQKFLDYAILGGRMGVSAVPGSGKTVTLSYLAAQLVK
                     EKIRDDQEVLIVTFANSAVDNFSRRIREFLQHEAELLPEVGYRVRTLHGLAHDVLRDR
                     PALLGLPEDFDIVDERVSGQILSDIVANWLRANPAFEFFVDPQIEQNRRAWLERERWP
                     DLVLQIAAAFIRRAKDLQMTPAAVHERMPSFEGDWLLSKMCLDIYQDYQRALAIRGGV
                     DFDDLTRLALEALQIDADLLARLQRKWPYILEDEAQDSSLLQERILRLLTEGGNWVRV
                     GDPNQSINTTFTTSNIRYLNDFLDEDDVVECPLLHSGRSTRRIVDLANTLVDWTLREH
                     PEETVRQRAFRDQRIILTPPDDAQPNPPDTPDLIVRLHDEALSPEKELDIVARSIQRW
                     LPEHRDRTVAILVPDNTRGFKVSEKLRSLGMEPDELLRSTSSTRQAAAKLRDVLRYLS
                     APLSSSFLARLFEMVNPLDPAEEADSQRVKDRKSIVTGLRRLDQVESFIWPRPDLDWL
                     AEFPPASDAAIRAELIDFRSVARRWLSASELPIDQLVLTVAQDLYRHGDADQIARELA
                     LAHKIAVVLRRLADDRPDWRLPDLAAELDRVANNEQRFLGFSEDDLGFTPRPGRVTVA
                     TMHKAKGLEWDRVYLLGVNNYDFPSALPGDVYRGQSWFVRDGLNLEAEAIGQLERMHE
                     GGGYVPGDATREARLAYTEERLRLLYVGITRARRELIITWNTGQRDDNPRQAAAPFLA
                     LYTYWESQQSLDAD"
     gene            complement(2431..4527)
                     /locus_tag="AC162G_06760"
     CDS             complement(2431..4527)
                     /locus_tag="AC162G_06760"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR5075547.1"
                     /translation="MPELINTTDLFKRIFLRGPAGTGKTTIAVERLRTLLEAGVRGES
                     ILVLAPQRSLLKPFQDELRKPDLPAGGQVETLTIGGLARRSVDLAWPAIAEAAGFARP
                     ERPPIFLTLETAQYHMARIVEPLIVERFYFDDVLLSRSRIYAQLLDSLNKSALVGFPY
                     TDVGRRLAAAWSGREARARIFVQVQDCVDRFRRYCLDNNLLDFSLQIELLAHHLLNLP
                     WFREDLFGRLRHLIVDNVEEDTPVTHDLLREWIPRSESALICYDADAGYRSFLAADPA
                     SAWDLHAVCDRTVKLVESRVVSIHVRALEYAMEHALSASGSVRRQTRGDARAALEFGG
                     GRFHPEMLDWVVRRIEHALNVEGVPPGEIVVLAPFLTDALRFALTDRLDRRGIPTRSL
                     RPSRALIEEPAARGLLTLSALAHRDWRVIPPRPDVAHALAMSIEGLDPARAHLLAERA
                     YHIADGAPSLRSFDQLRADLQDRVGYETGARYERLREWLAGYVASSPSTLDHFVSRLF
                     GEVLSQPGYGFHSAQGRARFDRGRIAAQVIESARKFRRVVAPDPFDAADALSIGRPYV
                     DLVSEGVIAATYVEPPGEAPQAALLAPAYTFLLGNEPATMQFWLNAGSPAWWERLYQP
                     LTHPYVLSRHWDLERKWTDEDEQAARRDTLLRLVRGLLRRCRGKVVLAYSSLNEQGFE
                     ERGPLLTLIQQVLREV"
     gene            4862..4999
                     /locus_tag="AC162G_06765"
     CDS             4862..4999
                     /locus_tag="AC162G_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075548.1"
                     /translation="MALKAKRELLASEVMPHFQRATLVEQQFCTGYGALRRTIGKYRL
                     P"
     gene            complement(4954..6216)
                     /locus_tag="AC162G_06770"
     CDS             complement(4954..6216)
                     /locus_tag="AC162G_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075549.1"
                     /translation="MKSIRVSARLSLVIALATIGWVMLVKSVPAAIVSESEPNDTPET
                     ANPLSNATASTGSISPTGDVDYYALGGLNTTWGFIALLDTISSTGSSAGQLQAIGSDG
                     SSVLQADSGSWERGSGIALQNYANGGDTHYLRVNEVGDDAAITSYTIRYYQTVVASQP
                     EAEPNETPTAGTPSSFTHSGTISPTGDVDCFAFHGRVGDTILLALNGDPENDGSPINP
                     VLELISPSDAVLKSVNVSGTAGREFIEYAGLPDTGVYAYCVRDGAGGGDPAATYRVGL
                     VRNGGLYFPSYTFSAIWLNPRPGNYALIDDLMSFRLSVTNTSPISIPGNIRITTDYSP
                     TCLSLVSVVPSATTSSTGYISWDGQKSGLAPGEVYSVTMNMRAQARCSDTVFQDLGLA
                     YYFIGTGGYADYVIYGSLYLPIVLRNAP"
     gene            complement(6270..7724)
                     /locus_tag="AC162G_06775"
     CDS             complement(6270..7724)
                     /locus_tag="AC162G_06775"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR5075550.1"
                     /translation="MEIRKQDFTKINDYLWEIPKSFRDDMRVPVRVYADDELLEDALG
                     DASMQQAMNVASLPTLVGYVVVMPDVHQGYGMPIGGVMASALPDGIISPGAVGYDVNC
                     GVRLLASRVSHDEVKDHIGDLATALYRNCPSGVGSEGHIRLSHDEFTDMLRQGSKWAL
                     KQGYARREDLDHTEEFGTMPGADPGKVSDRAVKRGRPQVGTLGAGNHFIEVDRVTSIF
                     DEEAARVLGLFEGQIVVQIHCGSRGFGHQVCTDYVSQFQSAVRKYGIQLPDRELVCAP
                     LDSPEGKDYLGAMACAANYAFANRQVLAHLVRRSFEEVLAGKVRDFDLHQIYDIAHNM
                     GKIEEHVIDGKSIKVCVHRKGATRAFGPGSDVLPPDLKPIGQPVLIPGSMGTASWVLL
                     GTAGSMEQSFGSTCHGAGRVMSRSRAKKEIRGEKLRDELQGRGIRVRAGSMPGLAEEA
                     PQAYKDVDRVIEVVSKAGIARKVARVEPMAVIKG"
     gene            complement(7771..8202)
                     /locus_tag="AC162G_06780"
     CDS             complement(7771..8202)
                     /locus_tag="AC162G_06780"
                     /inference="COORDINATES: protein motif:HMM:NF014058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0072669 - tRNA-splicing ligase complex
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HIR5075551.1"
                     /translation="MHVSTPDHYVQEIEHTADWAIRVRARDLIGLFAGAAEGMFSLLT
                     DLSQAIPVRQFEIDLRAIDVETLLIDWLNELLYLSEEHGVVFTGFTIRDLTIDGGARL
                     RAQVDGGHPSELFKVIKAATFHGLSIVRDDDGLQAELVFDV"
     gene            complement(8270..9316)
                     /gene="selD"
                     /locus_tag="AC162G_06785"
     CDS             complement(8270..9316)
                     /gene="selD"
                     /locus_tag="AC162G_06785"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006284830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(9266..9268),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR5075552.1"
                     /translation="MATEDVKLTALASCAGUASKFGPTDLAQVLRSMHNLFKPGDYPQ
                     VLVGLDGHNDAAIYRLNDELALVATTDFFPPVVDDPYAYGAIAAANSMSDVYAAGGEV
                     LFALNIVAFPEGLDHAILSEILRGGSEKVIEAGGVIIGGHTVSDKEPKYGLAVTGLVH
                     PDRFLTKRGARPGDRLILTKPLGVGLITTALKQGVAEAAHVEAAVQSMMRLNRHASHL
                     ALRFGAHACTDVTGFGILAHGLEVAEMSGAGLRFKMSDLPLLPGTRDVVTKEAFPGGL
                     WRNRDYVLPHVRFAESIREEDKLILYGPETSGGLLIALAPDRAQAYLEQARKDGMDAW
                     NVGEVMAGSGLEVV"
     gene            complement(9316..9681)
                     /locus_tag="AC162G_06790"
     CDS             complement(9316..9681)
                     /locus_tag="AC162G_06790"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR5075553.1"
                     /translation="MPVRSLTSSVLKWPDAKTVDQALRQWADPVARSRQDVLYIGYFG
                     SYARGDWGVGSDLDLIIVVEDSDRPFERRSVEWDTTALPVPTDVLVYTEDEWRSMAEE
                     RSSPGWRREIVWIYQRSRA"
     gene            complement(9638..10027)
                     /locus_tag="AC162G_06795"
     CDS             complement(9638..10027)
                     /locus_tag="AC162G_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR5075554.1"
                     /translation="MPNRAQDWFNQAVRDLDQAEDSRRAGRHEWACFAAQQAAEKAVK
                     ALHLHLGQEAWGHVVARLLSELPSVASAPDELIEKGRVLDGFYIPPRYPNSHPEGAPF
                     EHYGPLHSQEAIRYAREIIDFVRSQMA"
BASE COUNT         1911 a         3246 c         3110 g         1775 t
ORIGIN      
        1 ttccagcacg tagcgcagtt ggaaccggcg cgggcagtcg gcgtaatcgc tcaggctggc
       61 ttgcgagaat tggaaactag acgggagagt cattgtctgc gaaatctgcg ttaatctgcg
      121 tccaaagact gctgtgattc ccaataggta tatagtgcca ggaacggcgc cgcggcctgg
      181 cgcggattgt cgtcgcgctg gcccgtgttc caggtgatga tcagctcgcg cctggcacgc
      241 gtgataccga cgtacagcag gcgcagcctt tcctcggtat aggccagccg ggcctcgcgt
      301 gtggcgtcgc ccgggacgta gccgccgccc tcgtgcatcc tctcgagctg gccgatggct
      361 tcggcttcga gattcaggcc atcgcgcacg aaccacgact ggccacggta gacatcaccc
      421 ggcagcgcag atgggaagtc gtagttgttc acgccgagca gatagacgcg atcccactcc
      481 aaccccttcg ccttgtgcat cgtcgcaact gtgacgcgtc cgggccgcgg tgtgaagccg
      541 agatcatcct cgctgaaacc caggaagcgc tgctcgttgt tcgcaacccg atccagctcg
      601 gcggcgagat cgggcaagcg ccagtcgggc cggtcatccg ccagccgtct cagcacgacc
      661 gcaatcttgt gcgccagcgc aagctcgcgc gcgatctgat cggcgtcgcc gtggcgatac
      721 aggtcctgcg cgacggtcag aacgagctgg tcgatcggca gttccgatgc actcagccag
      781 cgacgagcga cgctgcggaa atctataagc tcggcgcgga tggccgcatc cgacgcgggc
      841 ggaaactcag ccagccagtc gagatcggga cgcggccaga tgaacgactc gacctggtcg
      901 agccggcgca ggccggtcac gatcgatttg cgatctttga cgcgttgcga atccgcttcc
      961 tccgccggat cgagagggtt gaccatctca aacagcctgg cgagaaatga gctggacagc
     1021 ggcgccgaca ggtaacgcaa aacgtcacgc agcttcgcgg ctgcctgacg ggtcgaggac
     1081 gtgctgcgca gcagctcgtc aggctccatg cccagcgagc ggagtttctc gctgaccttg
     1141 aagccgcgcg tgttgtcagg aaccaggatg gcgaccgtgc gatccctgtg ctcaggcagc
     1201 cagcgctgga tcgacctggc gacgatgtca agctccttct ccggcgacag cgcctcgtcg
     1261 tgaaggcgca cgatcaggtc gggtgtatcg ggagggttcg gctgggcatc gtcgggcggc
     1321 gtcagaatga ttctttgatc gcgaaaggcg cgctgccgaa ctgtctcctc cgggtgttcg
     1381 cgcagggtcc agtcgacgag ggtgttcgcc aggtcgacga tccggcgcgt cgagcggccg
     1441 gagtgcagca aggggcactc gaccacgtca tcttcgtcga gaaaatcgtt caggtagcgg
     1501 atgttggacg tcgtgaacgt cgtgttgatc gactggttgg gatcgccgac gcgcacccag
     1561 ttgccgccct ccgtcagcag tcgcaagatt ctctcctgaa gcaaactgct gtcctgcgct
     1621 tcgtcttcca gaatgtacgg ccacttacgc tgcagccgcg ccaggaggtc ggcgtcgatt
     1681 tgcagcgctt cgagcgccag gcgcgtgaga tcgtcaaagt cgacgccgcc gcgtatcgcg
     1741 agcgcacgct gataatcttg gtagatgtcg agacacatct tgctcagcag ccagtcacct
     1801 tcgaacgagg gcatgcgctc gtgcacggca gccggcgtca tctgcaggtc tttcgcgcgc
     1861 cggataaagg ccgccgcgat ctgcagaacc aggtccggcc agcgctcgcg ctcgagccag
     1921 gcgcgccggt tttgctcgat ctgcggatcg acgaagaact cgaacgccgg gttagcgcgc
     1981 agccagttcg cgacgatgtc tgagaggatc tgccccgata cccgctcgtc cacgatgtcg
     2041 aagtcttctg gcaggccgag cagcgccggc cggtcgcgca agacgtcgtg cgccaggccg
     2101 tgcagcgtgc gcacgcgata gccgacctcg ggcagcaatt ccgcctcgtg ctgcaggaac
     2161 tcgcggatgc ggcgcgagaa gttgtcgacg gccgagttgg cgaaggtgac gatcagcact
     2221 tcctggtcgt cgcggatctt ctccttcacg agctgcgcgg cgaggtaaga cagcgtgacg
     2281 gtcttgcccg accccggcac agccgacacg cccatccgcc cgccgaggat cgcgtagtca
     2341 aggaatttct gttgggaagg acgtggtgta aacatttcgg gatgcagatt gcacagatga
     2401 acgcagatta tttctgcgct catttgcgtc ctatacttct ctgagtacct gctgaattaa
     2461 cgtcagtaag gggccgcgct cttcgaagcc ttgctcgttc aggctgctgt aggcgagcac
     2521 aactttgccc cggcagcggc gcaataagcc gcgtaccagg cgcagcagcg tatcgcggcg
     2581 cgccgcctgc tcgtcttcgt cggtccactt gcgctcaagg tcccagtgcc ggctgagcac
     2641 atacgggtgc gtcaggggct gatacagccg ctcccaccac gccgggctgc ccgcattcag
     2701 ccagaactgc atcgtcgcgg gctcgttgcc gagcaagaag gtgtacgccg gcgcaagcag
     2761 ggcagcctgc ggcgcttcgc ccggcggctc gacgtacgtc gcggcaatca cgccttcact
     2821 caccagatcg acgtaaggcc tgccgatcga caaagcgtcg gcggcgtcga acggatcggg
     2881 cgcgacgacc cgccggaact tgcgtgcgga ttcgatgacc tgcgcggcga tgcgaccgcg
     2941 atcgaaccgg gcgcgcccct gcgcggagtg gaagccgtaa ccaggctgcg acagcacctc
     3001 gccgaacagg cggctcacga agtggtcgag cgtcgacggc gaagacgcga cgtagccggc
     3061 cagccattcg cgcaggcgct cgtaacgcgc gcccgtctcg tagccgacgc ggtcttggag
     3121 atcggcgcgg agttgatcga acgatcgcag cgacggcgcg ccatcggcga tgtgataggc
     3181 gcgctcggcc agcaggtgcg cgcgcgctgg atccagcccc tcgatcgaca tcgccagcgc
     3241 gtgcgccacg tccggtctgg gcgggatgac gcgccagtca cgatgcgcca gcgccgacag
     3301 cgtgagcagg ccgcgtgcgg ccggttcctc gatcagcgcg cgagaggggc gcagcgaacg
     3361 ggttgggatg ccccgccgat ccagccggtc ggtcagcgcg aaccgcagcg cgtcggtcag
     3421 gaacggcgcc aggacgacga tctcgcccgg cgggacgccc tccacgttga gggcgtgttc
     3481 gatacgccgc acgacccaat ccagcatctc gggatggaag cgaccccccc cgaattccag
     3541 cgcggcacgc gcatcgccgc gcgtctggcg gcgcaccgag ccagaagcag atagcgcgtg
     3601 ctccatcgcg tactcgagcg cgcgcacgtg gatcgagacg acgcgcgact cgactagctt
     3661 gacggtgcga tcgcataccg catgcaagtc ccaggctgac gccggatcgg cagccaggaa
     3721 cgagcgatag cccgcgtcgg cgtcgtagca gatcagcgcc gactccgagc gcgggatcca
     3781 ctcgcgcagc aggtcgtgtg tgaccggtgt gtcctcctcg acgttgtcga cgatgagatg
     3841 ccgcaggcga ccaaataaat cctcgcgaaa ccacggcaga ttgagcaggt ggtgcgccag
     3901 caactcgatt tgaagcgaga aatccaacag gttgttgtcg agacagtagc ggcgaaagcg
     3961 atcgacgcag tcctgcacct gcacgaagat gcgagcacgc gcctcccggc ccgaccacgc
     4021 tgcggccagg cggcgcccga cgtcggtgta ggggaatccg acgagcgccg atttattcag
     4081 actatcgaga agctgagcgt agatgcggct gcgcgagaga agcacgtcgt cgaagtagaa
     4141 gcgctcgacg atgagcggct cgacgatgcg cgccatgtga tactgcgccg tctcgagtgt
     4201 gaggaagatc ggcggccgct cgggtctggc aaatccggcc gcctcggcaa tcgccggcca
     4261 ggcaagatcg acggagcggc gagcgaggcc gccgatcgtc agcgtctcga cctgtccgcc
     4321 ggccggcaaa tcgggcttac gcagctcgtc ctgaaacggc ttgagcagcg agcgctgtgg
     4381 agcgaggacc aggatcgact cgccgcgcac accggcttcg agcagtgtac gcaagcgctc
     4441 gacggcgatc gtcgtcttcc ccgtgccggc cggcccgcgc aggaagatac gcttgaatag
     4501 atcggtcgtg ttgatcagct caggcataat cggcaattat agtccaaagt cgatgaaaga
     4561 catccccctc tggagacagc caggattcgg ctggcgacgc tcgtcacgtc ggtcgcctat
     4621 cgcagcccgg cgctgttggc gaaaatggcg gccggcatcg acatcctgat cggcggcagt
     4681 ggcgagcaac tgacgctgcg cgcgctcgcg cgcgtcgggg acgcctgcaa catcttcggc
     4741 gatcccgatt cggtgaaacg caagttcgac gtgccgcggc atgactgcga ggccgagggg
     4801 caaaacttcg aggacatcga gcgaacgaac cctgaccagc ctgctgttgg cacgagacga
     4861 agtggcactc aaggccaagc gcgaactgct ggcgagcgag gtgatgccgc acttccagcg
     4921 agccacgctc gtcgagcagc agttctgcac tggctacggc gcgttgcgca ggacgatcgg
     4981 caagtacagg ctgccataga tcacatagtc ggcatacccg ccggtgccga tgaaatagta
     5041 agccagcccc aggtcttgaa agaccgtgtc gctgcatctg gcctgggcac gcatgttcat
     5101 cgtcacacta tagacttcgc caggggcgag ccctgatttc tgaccgtccc acgagatgta
     5161 tcctgtcgag gacgtcgtcg cggatggcac cacactaacc aggctcaggc aggtgggtga
     5221 gtaatctgtg gtgatgcgga tattgccagg gatcgagatc gggctggtgt tggtcactga
     5281 caaccggaag gacatcaagt cgtcgatcag cgcatagttg cccggtcgcg ggttcagcca
     5341 gatcgcgcta aacgtgtaac tcgggaagta cagaccaccg ttgcgcacca gtcccacgcg
     5401 atacgtggct gccgggtcac ctccgccggc tccatcccgc acacagtacg catacacccc
     5461 cgtgtcgggc aatccggcgt actcgatgaa ctccctgcct gccgtgccag atacgttgac
     5521 cgatttgagg acggcatcgg atgggctgat caattccaat accggattga tcggactgcc
     5581 atcgttttcg ggatcaccat tcagtgccaa caagatcgta tcacccacgc ggccgtggaa
     5641 ggcgaagcag tccacgtcgc cggtggggct gatcgtgccg ctgtgcgtaa acgacgacgg
     5701 ggtgccagcc gtgggcgtct cgttaggctc tgcctcgggc tggctcgcaa cgaccgtctg
     5761 gtagtaacga atcgtgtacg aggtaatcgc cgcatcgtct ccaacctcgt tcacgcgcag
     5821 ataatgtgtg tcgccgccgt tcgcgtaatt ttgcagcgcg atgcctgatc cgcgttccca
     5881 cgatccagaa tcggcctgca ggacagacga gccatcacta ccgatggcct gcagctgccc
     5941 cgcactgctc ccggttgaac tgatggtatc gagcagcgca atgaagcccc acgtcgtatt
     6001 caggccgccc agcgcatagt agtccacatc accggtagga ctaatcgagc cagtactcgc
     6061 cgtcgcgttg gataatgggt tggccgtctc cggtgtatca ttcggctcgc tctccgacac
     6121 gatcgcagcc ggaacagatt tgacgagcat cacccacccg atggtcgcca gggcaatgac
     6181 caggctgagt ctggcggaca ctctgattga cttcatcgtt cattctccgc ttgaggggga
     6241 tgtttgccgc gcgccattgt agcacatact cagcccttga tcaccgccat cggctccacg
     6301 cgcgccacct tgcgggcgat gcccgccttc gacacgacct cgatcacccg atccacgtcc
     6361 ttgtacgcct gcggcgcttc ctcggccagc cccggcatcg acccggcgcg cacccggatc
     6421 cccctgccct gcagctcgtc gcgcagcttc tcgccgcgaa tctccttctt ggctcgcgag
     6481 cggctcatca cccggcccgc gccgtgacag gtcgatccga acgactgctc catcgacccg
     6541 gccgtgccca gcagcaccca cgaggctgtg cccatcgatc cggggatcag caccggctgg
     6601 ccgatcggct tgaggtcggg cggcagcacg tcggagccgg ggccgaaggc gcgcgtcgcg
     6661 cccttgcgat gcacgcacac cttgatcgac ttcccatcga tgacgtgctc ttcgatcttg
     6721 cccatgttgt gggcaatatc gtagatttga tgcaggtcga aatccctgac cttgccggcc
     6781 aacacctcct cgaacgagcg ccggacgaga tgcgccagca cctgccggtt ggcgaaggca
     6841 tagtttgcgg cacaggccat cgcgccgaga tagtctttgc cttcaggcga atcgagcggc
     6901 gcgcacacca gctcgcgatc gggcaactgg atgccgtact tgcgcacggc ggactgaaac
     6961 tggctgacgt agtcggtgca cacctggtgg ccgaagccgc ggctgccgca gtgaatctgc
     7021 acgacgatct ggccctcgaa cagccccagc acacgcgcgg cttcctcgtc gaagatcgac
     7081 gtgacgcgat cgacctcgat gaagtgattg cccgcgccga gcgtgccgac ctgggggcga
     7141 ccacgcttga cggcgcggtc gctgactttg cccggatcgg cgcccggcat cgtgccgaat
     7201 tcctcagtgt ggtcgaggtc ttcgcgccgc gcgtagcctt gcttgagcgc ccacttcgac
     7261 ccctgccgca gcatgtcggt gaactcatcg tgcgaaagcc gaatgtgccc ttccgagccg
     7321 acgccactcg ggcagttgcg atacagcgcc gtcgccaggt cgccgatgtg atctttgact
     7381 tcgtcgtgcg agacgcgtga ggcaaggagc ctcaccccac agttgacgtc gtagcccacg
     7441 gcaccaggcg aaataatccc atccggtagc gcggatgcca tgactccccc aataggcata
     7501 ccgtatcctt gatgaacgtc cggcatgacc accacgtatc ctactagcgt gggcaggctc
     7561 gccacattca tcgcttgctg catcgaagca tcgcccaacg cgtcttcgag cagctcgtcg
     7621 tcggcgtaca cccgcaccgg cacgcgcatg tcatcgcgga acgatttggg aatctcccac
     7681 agataatcgt tgatcttggt gaaatcctgt ttgcggatct ccatgataca cctccggaga
     7741 cctgtgaggt ctggcagacc tcacaggtct tcacacgtcg aacaccaact cggcctgcaa
     7801 cccgtcgtcg tcgcggacga tcgacagccc atgaaacgtc gccgccttga tcaccttgaa
     7861 taactcggac gggtgaccgc catcaacctg cgcccgcagc cgagcgccgc catcgatagt
     7921 tagatcacgg atcgtaaagc cggtaaatac cacgccatgc tcttctgaga ggtatagtag
     7981 ttcattcagc cagtcaatga gcagcgtctc gacgtcgatc gcccgcagat cgatctcaaa
     8041 ctgccgaacg gggatggcct gagacaggtc ggtgagcagg ctaaacatgc cctccgccgc
     8101 cccggcgaac aagccgatca agtcgcgtgc acggacacga atcgcccaat cggcagtatg
     8161 ttcgatttcc tgaacgtagt gatccggagt actcacatgc acagtataca cctatagaaa
     8221 agaaacgccg cacctggcag ggcggcgctc gaatcgaacg gcgctagcct cacaccacct
     8281 caaggcccga tccggccatc acctcgccga cgttccaggc atccatccca tctttccttg
     8341 cctgttccag gtatgcttgc gcccgatcgg gcgcaagcgc tatcagcagt ccacccgacg
     8401 tttccgggcc atacaagatc aacttatcct cttcgcggat cgattcggcg aagcgcacgt
     8461 ggggcaggac atagtcgcga ttgcgccaca gaccgccggg gaacgcttcc ttcgtcacaa
     8521 catcgcgcgt gccgggcagc agcggcaaat cggacatctt aaagcgcagg ccggcgcccg
     8581 acatttcggc aacttccagc ccgtgagcca ggatgccgaa gccggtcacg tcggtgcagg
     8641 cgtgggcgcc gaagcgcagc gccaggtgcg aggcgtggcg gttcaaccgc atcatgctct
     8701 gcacggctgc ctcgacatgc gccgcttcgg cgacgccctg cttgagcgcg gtcgtaatca
     8761 ggccgacgcc gagcggcttc gtcaagatca ggcggtcgcc tggccgtgcg ccgcgcttgg
     8821 tcaggaagcg atcggggtga accaggccgg tcaccgccaa cccgtacttc ggctctttgt
     8881 cagagaccgt atgcccgccg atgatcacgc cgccggcctc gatcaccttc tcgctcccgc
     8941 cgcgcagaat ctcggacagg atagcgtggt caagcccttc gggaaaagcg acgatgttga
     9001 gcgcgaacag cacttcgccg ccggctgcgt acacgtcgct catcgagttg gccgccgcaa
     9061 tcgcgccgta ggcgtaggga tcgtcgacga ctgggggaaa gaagtcggtc gtggcgacca
     9121 gggcaagctc gtcgttgagg cggtaaatgg ccgcatcatt atggccgtca agtccgacca
     9181 ggacctgcgg atagtcgccg ggtttgaaca gattgtgcat ggagcgcagc acctgcgcca
     9241 ggtcggtcgg gccgaacttg ctggctcaac ccgcgcacga cgccagcgct gtcagcttaa
     9301 catcctcagt tgccatcaag ctctgctccg ctgataaatc catacgatct ctcgtcgcca
     9361 gccgggcgac gagcgctctt cggccatgga gcgccactcg tcctctgtat acaccagcac
     9421 atcggtcggc accggcaggg ccgtggtatc ccactcgacc gagcgtcttt caaatggccg
     9481 atcgctatct tcgacgacga tgatcaggtc aaggtcgcta ccgacgcccc agtcgccgcg
     9541 agcatatgag ccaaaataac cgatgtacag cacatcttgc cggctgcgtg cgaccggatc
     9601 agcccactgg cgcaaggctt gatcaaccgt cttcgcatca ggccatttga gaacggacga
     9661 agtcaatgat ctcacgggca tatcgaatgg cctcctggct gtgcagcggc ccgtaatgct
     9721 cgaagggcgc tccttctgga tgactgttcg ggtagcgcgg cggaatgtaa aagccgtcaa
     9781 gcacgcggcc cttctcaatc aattcgtccg gagcggacgc aacgcttggc agttcgctca
     9841 aaagcctggc gaccacgtgc ccccaggctt cttggccaag gtgaaggtgc agagccttca
     9901 ccgccttttc ggctgcctgc tgcgctgcaa agcaggccca ctcgtggcgg cctgcccgcc
     9961 gggagtcttc agcctgatcc agatccctca cggcctggtt aaaccaatct tgagcgcggt
    10021 taggcacgtt gctttacccc ca
//
LOCUS       DBNHXB010000171         5647 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_542520, whole genome shotgun sequence.
ACCESSION   DBNHXB010000171 DBNHXB010000000
VERSION     DBNHXB010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_542520"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2350
                     /locus_tag="AC162G_06800"
     CDS             <1..2350
                     /locus_tag="AC162G_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR5075555.1"
                     /translation="GARAGYAFWMGFDRPSPDHAHANVILLISSHLETGHYFNPHAQR
                     IIEGKMAGAKLIVFDTRLSNTASMADTWIAPWPGSEAAILLAIANYLITTGKYNRDFV
                     RKWVNWEEYLKSQTPNSKLQNPNHQLLITFEQFEAALKDQYAKYTFEFAAQESGVEAR
                     ILKGIAEDVANCGGKLASHNWRSAGAGNLGGWQVARCLWFLNVLTGSVGVEGGTSANA
                     WDKWVPRPHKMAPHVKCWNELTWPREYPLSYFEMSFLLPHFLKEGRGKLDVYFTRVYN
                     PMWTNPDGFTWLEVLRDESKIGLHVALTPTWNESAWWADYVLPMGHASERHDLMSQET
                     HAGQWIAFRQPVMRAAMERMGKKVNRTYEANPGQVWEETEFWIDLSWEIDPDGSLGIR
                     PYFESPYRPGERITVDEYYGWMFENSVPGLPEAAAKEGLSPLAYMRKYGAFEIRKGAQ
                     AEYDRTLTPEEIVDAQVDEETGIIYTKAPMAPPKNIVPAPAPVLTELGRPVGVLIDGE
                     AKFGFRTPSRKLEFYSMTLRDFGWPELAIPEYVESHVHPSHVNHEANEFVLLSTYRLP
                     TLIHTRSANAKWLVEISHSNPTWMHTSDASRLGVQTGDLVRVETEIGYFVDKVWVTEG
                     IRPGVIACSHHLGRWRLAEGEGTNRIASALVELKESVARGEWRLTQLKGVQPYKSSDP
                     DTERIWWSDAGVHQNLTFPVQPDPISGAHCWHHKVRVARAQPGDRYGDVFVDTQKSHE
                     VYKRWLAMTRPPGGEWRRPNWMLRPFRPDVSAYRRYHVADGR"
     gene            2498..3403
                     /locus_tag="AC162G_06805"
     CDS             2498..3403
                     /locus_tag="AC162G_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorD/DmsD family molecular chaperone"
                     /protein_id="HIR5075556.1"
                     /translation="MSRARQIPALAPYGSEPGELATAYADLFLLNVYPYASVFLDPDG
                     EMNGTRAQEMQDVYEQYGCAPDALHDVGAPDHIGLMLGLLAHEGRDAHSIPSALRAGL
                     PYLLDWAPVLCLAVEREPAAHSFYRALASETRRVLFAEIESPDIEPRVRTSGHSAEPE
                     LELELELELESGLDPSALRAGFSGEEISVRSIVHRLLCPARSGVFLSRARLGAWARDL
                     GVPLPFGGRFDVGISLFEAAGVVDSTARLVDWLIAEVEEWDAAFMAWAEAGWRDYAVA
                     WRARITRTARLLAEMRELLLSTSPT"
     gene            3518..4393
                     /locus_tag="AC162G_06810"
     CDS             3518..4393
                     /locus_tag="AC162G_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075557.1"
                     /translation="MAARRDTLGEPPTFDEYLELRASFAVTRDLAQRALAWAQPFAME
                     ALGIHAQRQTLVDGELPAYVVPTAPETNGGWGELMPVWFKPYDRPVPLHCLDNWALFA
                     LLYRSAFGRDADLSRFSVENLARVVLGADLLIPFLPGTDGAGRFLRHGALPILLAILW
                     LEPDVRDAWLALHERGARKFATDHDLSHVLSEAEGAVENLGEWLERVENEAWLVPERG
                     MVAGKAAVADFLADTVRASQYASLFGGLCLGVSALAAAWGNDWRKWVFQAVNVGYRLP
                     DFGLPDITTWLKSLS"
     gene            complement(4455..4883)
                     /gene="hepT"
                     /locus_tag="AC162G_06815"
     CDS             complement(4455..4883)
                     /gene="hepT"
                     /locus_tag="AC162G_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII toxin-antitoxin system HepT family
                     RNase toxin"
                     /protein_id="HIR5075558.1"
                     /translation="MVDPNTVASILNNLRSYQDKLSTLARYSKDDFLRDFTKVESAKH
                     LLQVSIESCLDIAHHVVADEGFRTPQDSYDAFVVLNEQGILPNDFMPALRQMVSFRNR
                     VVHLYWDVDAATVYDIVQHNLGDFEAFTDHILRFLQPPSE"
     gene            complement(4846..5352)
                     /gene="mntA"
                     /locus_tag="AC162G_06820"
     CDS             complement(4846..5352)
                     /gene="mntA"
                     /locus_tag="AC162G_06820"
                     /inference="COORDINATES: protein motif:HMM:NF047752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII toxin-antitoxin system MntA family
                     adenylyltransferase antitoxin"
                     /protein_id="HIR5075559.1"
                     /translation="MVEQPYIRIERQRHAAQIIDRLKAHIADVLAAYPVEMAYLYGSV
                     ARGRPLPSSDVDIALVLTESPPPYRQLMLELEIQAALENACGLSNLDVRAINHAPLTV
                     QGRIIQEGLLLYERDKARRVTFEALTRRRYFDYQPTAERMRRAFLDHVRRKGLTGGRS
                     QHRRIYSE"
     gene            5463..>5647
                     /locus_tag="AC162G_06825"
     CDS             5463..>5647
                     /locus_tag="AC162G_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HIR5075560.1"
                     /translation="MPSNPKTNSSRRQAGQLGGRATCEQYGLDHYRAIGSLGGQTTMS
                     RHADEYAVYRQRGGQAT"
BASE COUNT         1080 a         1700 c         1803 g         1064 t
ORIGIN      
        1 cggcgcgcgc gcgggctacg ccttctggat gggcttcgac cgccccagcc cggatcacgc
       61 ccacgccaac gtcatcctgc tcatcagcag ccacctcgaa acagggcact acttcaatcc
      121 gcacgcccag cgcatcatcg aaggcaagat ggccggagcg aagctgatcg tgttcgacac
      181 acgcctctcg aacaccgcgt cgatggcaga cacgtggatt gcaccgtggc ccggctcgga
      241 agcggcgatc ctgctcgcca tcgccaatta cctgatcaca accggaaagt acaaccgcga
      301 tttcgttcgg aagtgggtga attgggaaga gtatcttaaa tctcaaactc caaactccaa
      361 actgcaaaat cccaatcacc aattactaat taccttcgaa caattcgaag cggcgctcaa
      421 agatcaatac gccaaataca ctttcgaatt cgcggcgcag gagtcgggcg tcgaagcgcg
      481 catcctcaag ggtatcgccg aagacgtcgc gaactgcggc ggaaaactgg cgtcgcacaa
      541 ctggcgcagc gcgggcgcgg gcaacctcgg cggctggcag gtggcgcgct gcctgtggtt
      601 cctcaacgtg ctcaccggca gcgtcggcgt cgagggcggc acgtcggcga acgcgtggga
      661 taagtgggtt ccacgcccac acaagatggc cccgcacgtc aagtgctgga acgagttgac
      721 ctggccgcgc gagtacccgc tgtcgtactt tgaaatgagc ttcctgctgc cgcactttct
      781 caaggagggg cgcggcaagc tcgacgtgta cttcacgcgc gtctacaacc cgatgtggac
      841 gaaccccgac ggcttcacct ggctcgaagt gctgcgcgac gaatcgaaga tcgggcttca
      901 cgtcgcgctc acaccgacgt ggaatgaatc ggcgtggtgg gccgactacg tcctcccgat
      961 ggggcacgcc tcggagcggc acgacctgat gagccaggaa acgcacgccg gacaatggat
     1021 cgcctttcgt cagccggtca tgcgtgcggc gatggagcgc atggggaaga aggtgaaccg
     1081 gacgtacgag gcgaatcccg gccaggtgtg ggaggagacg gagttctgga tcgacctgag
     1141 ctgggagatc gaccccgacg gatcgctcgg catccggcca tatttcgagt cgccgtatcg
     1201 gcccggcgag cgcatcaccg tcgacgagta ttacggctgg atgttcgaga attcggtgcc
     1261 gggcttgccg gaagctgccg cgaaggaagg gttgtccccg ctggcttaca tgcgcaagta
     1321 cggcgcgttc gagattcgca aaggcgcgca ggcggagtac gaccgcacgc tcactccgga
     1381 agaaatcgtc gatgcgcagg tcgacgagga gacgggcatc atctatacga aagcgccgat
     1441 ggcgccgccg aagaatatcg tgcccgcgcc agcgcctgtc ctgaccgagc tgggccgccc
     1501 cgtcggggtg ctgatcgacg gcgaggcgaa gttcggcttc cgcaccccat cgcgcaagct
     1561 cgaattctat tcgatgacgc tgcgggactt cggctggccc gagcttgcca ttcccgaata
     1621 cgtggagagc cacgtgcacc ccagccacgt caaccacgag gcgaatgaat tcgtcctgct
     1681 ctcgacgtac cgcctgccga ccctgattca cacgcgcagc gcgaacgcca agtggctggt
     1741 cgagatctca cattcgaacc cgacgtggat gcacacgtcg gacgcgtccc gcctcggggt
     1801 gcagacgggc gacctggtgc gcgtcgagac ggagatcggc tatttcgtcg acaaggtttg
     1861 ggtcaccgag ggcattcgcc ccggcgtgat cgcttgctcg catcacctcg gccgctggcg
     1921 attggccgaa ggcgagggga cgaaccgcat tgcatcggcg ctcgtcgagc tgaaagaatc
     1981 cgttgcgcgc ggtgaatggc gactcactca gctcaaaggc gtccagccct acaagtcgtc
     2041 cgatccggac accgagcgca tctggtggtc ggatgcgggc gtgcatcaga acctcacctt
     2101 tccggtgcag cccgatccga tcagcggcgc ccactgctgg catcataagg tgagagtcgc
     2161 ccgcgctcag ccgggcgacc gttacggcga cgtgttcgta gacacgcaaa agtcgcacga
     2221 ggtttacaag cgctggctgg cgatgacacg cccgccgggc ggcgagtggc ggcggcccaa
     2281 ctggatgctg cgcccgttcc ggcccgacgt gagcgcgtat cgccggtatc atgtggctga
     2341 tggccgataa cagatagctg atggcaggct ggtgactggt gactgatgac tgataactgg
     2401 tgactgatga ctgatgactg ataactgatg accgatgact gaattcacgt tttctcttct
     2461 ttctgcgctt tggcttcgag agccggatgc tgagacgttg tctcgcgcac gccagattcc
     2521 tgcgcttgct ccctatggca gtgaaccggg agagctggcg acggcctacg ccgacctgtt
     2581 cttgctcaac gtatatccct atgccagcgt gttcctcgac ccggacgggg agatgaacgg
     2641 gacgcgggcg caggaaatgc aggacgtcta cgagcaatac ggctgcgcgc cggacgcgct
     2701 gcacgacgtg ggggcgccgg atcacatcgg gctgatgttg ggattgctcg cgcatgaagg
     2761 acgtgatgcg cactccatcc cttcggctct cagggcaggc ttgccgtatc tcctcgactg
     2821 ggcgcctgtc ttgtgcctgg ccgtcgagcg cgagccggcc gcgcacagct tctatcgcgc
     2881 cctggcctcg gagacccggc gcgttctgtt cgccgaaatc gagtcgcccg atatcgaacc
     2941 acgcgtgcga acgagcggcc acagtgcgga gccagagtta gagttagagt tagagttaga
     3001 gttagagtca gggttggacc cttcggcact cagggcaggg ttttcgggtg aggaaatttc
     3061 cgtgcgctcg atcgtccacc ggctgctgtg cccggcgcgc agtggggtct ttctgtctcg
     3121 cgcccggctg ggtgcgtggg cgcgcgacct gggtgtccct ctgccattcg gtggccgatt
     3181 cgacgtcggg atctcgctgt ttgaagcggc tggggtcgtg gacagcacgg ctcgcctggt
     3241 ggactggctg atagcggaag tcgaggagtg ggacgcagcc ttcatggcat gggcggaagc
     3301 aggatggagg gactacgccg ttgcgtggcg tgcgcgtatc acgaggacag ctcgactgct
     3361 ggctgaaatg agagaactcc tcctgtcgac ctcgcccacc taatgcagcg cggcggaaat
     3421 aaccccacac gtggagtgtg tcatgctgaa tggagtgaag catcccggag tgaagcatcc
     3481 cgccgccgga ttcacgccga gcatgtgatc gacagcgatg gcagccaggc gtgacactct
     3541 cggagaaccg cccacgttcg acgaatacct cgaactgagg gcgtctttcg ccgtgacgcg
     3601 cgatctcgcc caacgcgcgc tggcctgggc gcagccgttt gcgatggagg ccctcggcat
     3661 tcatgcccaa cggcagactc tcgtcgatgg cgagctgcct gcttacgtcg tgccgacggc
     3721 gccggagacg aacggcggct ggggcgagct gatgcccgtc tggttcaagc cttacgatcg
     3781 accggtgccg ctgcactgcc tcgacaactg ggcgctgttc gcgctgctgt atcgctcggc
     3841 cttcggtcgc gacgcggacc tgtcgagatt ctccgtcgaa aacctggcgc gggtcgtcct
     3901 cggcgcagac ctgctcatcc cattcctgcc gggcaccgac ggcgctgggc gctttctgcg
     3961 gcatggcgcg ctccctatcc tgctcgcgat cttgtggctt gagcctgacg tgcgggatgc
     4021 ctggctggcg ctgcacgagc gcggcgcgcg aaagttcgcc acggaccacg acctgagcca
     4081 tgtcctgagc gaagccgaag gagccgtcga gaacctgggc gaatggctgg agcgcgtcga
     4141 gaacgaggcg tggctcgtgc ccgagcgcgg aatggtcgca ggcaaggcgg ctgtagccga
     4201 ttttctcgcc gacacggtgc gcgcttcaca gtatgcctcc ctgttcggtg gtctgtgcct
     4261 gggcgtctca gccctggcag ctgcctgggg caatgattgg cgtaagtggg ttttccaggc
     4321 cgtgaacgtc ggttatcgcc tgccggactt tggcctgcct gacatcacca catggctgaa
     4381 gagtctatca tgaagccact tgtggcgtgg caaccgcgag gccgcgagct atgagcaaat
     4441 ctgacattgt caaatcactc agatggtggt tgcagaaatc tcagaatatg atcggtgaaa
     4501 gcctcgaagt cgcccaggtt gtgctgcaca atgtcgtaaa cggtggctgc gtccacatcc
     4561 cagtagaggt gcaccacccg gtttcgaaaa ctgaccattt gacgcagcgc cggcataaaa
     4621 tcgttcggca ggatgccttg ttcgttgagc accacaaatg cgtcgtagct gtcttgcggc
     4681 gtccgaaaac cctcgtcggc gaccacgtga tgagcgatgt ccaggcacga ttcgatggat
     4741 acttgcaaca agtgcttggc gctttctact tttgtgaagt cccgaaggaa gtcgtctttg
     4801 gagtaccggg ccaaagtgga tagcttgtcc tggtagctgc gcaggttatt cagaatagat
     4861 gcgacggtgt tgggatcgac caccggtcag cccctttcta cggacgtggt ctaaaaaggc
     4921 ccgtcgcatc cgctcggccg ttggctgata gtcgaagtac ctccggcggg tcagtgcctc
     4981 gaatgtcacg cggcgggctt tgtctcgctc gtagagcagc agcccttcct gtataatccg
     5041 accctgtact gtcagcggag cgtggttgat tgcccgcacg tccaggttgg acaggccaca
     5101 ggcattctcg agggcggctt gaatctctaa ttcgagcatc aattgccggt aaggcggcgg
     5161 cgactcggtc aacaccaacg caatgtccac gtcactcgaa ggtaacgggc ggccccgggc
     5221 caccgatcca tacaggtagg ccatttcaac cgggtatgcg gccagaacat cagctatgtg
     5281 ggctttgagc cggtcgatga tctgagctgc gtggcgctgt cgctcgattc ggatgtatgg
     5341 ctgctcgacc atgccgatat tataccgcgc tcacggtcag ggatgaagtg tacagcattg
     5401 acagcggctc aattctcatt tatactatct gcggctgacc aagccaaaca aaggggcctg
     5461 acatgccgtc caatccgaag acgaactcat ctcgccggca agccggccaa ctcggcggca
     5521 gagcgacgtg cgagcagtac ggcctcgacc attaccgggc gatcggcagc ctgggcggcc
     5581 aaacgacgat gtcgcgccat gccgacgagt acgcggtcta tcgccagcgc ggcgggcagg
     5641 cgacgaa
//
LOCUS       DBNHXB010000172         3468 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2388320, whole genome shotgun sequence.
ACCESSION   DBNHXB010000172 DBNHXB010000000
VERSION     DBNHXB010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2388320"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="AC162G_06830"
     CDS             <1..237
                     /locus_tag="AC162G_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013507.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kelch repeat-containing protein"
                     /protein_id="HIR5075561.1"
                     /translation="ADGQIMVAGGEVIISGRETLSSLEVFDPGDNAWRLGPDLPAPIH
                     GVGGASLGGRFFLLGGSREAGGIANSGTVQIYLP"
     gene            259..1644
                     /locus_tag="AC162G_06835"
     CDS             259..1644
                     /locus_tag="AC162G_06835"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5075562.1"
                     /translation="MKRQGRLIALVLLSAVFNACAAPVATPAATSVRTVSPPEAIALA
                     DEFYSTLVVERDFAGATENFDSVMLSALPEAKLEETWATIVAQVGAYQERLADPTVER
                     VDRYERVTIALKFERAVLDMQVVVDSTTGLISGLFFVPSRQASLLYTTPGYADPDTFE
                     EQDVTVGSGEWTLPGTLTMPKGEGPFPAVVLVHGSGPNDRDETLGPNKPFKDLAWGLA
                     SQGIAVLRYEKRTKVYADRLVSLDGEFTVREETIDDALAAASLLRERQEIDPGRIVVL
                     GHSLGGMLAPRIAQAGPDVAGLIVMAGPARPLEDLILEQARTIVSQSGKPSLQEQQQI
                     QAIEQQVQAIKALQPSGDGSGKEMLLGAPASYWLDLRGYDPAEAAGVLTQPMLILQGE
                     RDYQVSMADFRRWQDVLSSRPRVRFKSYPELNHLFIAGSGPSAPEEYQTPGHVAAYVI
                     DDLASWIAQLP"
     gene            1656..2582
                     /locus_tag="AC162G_06840"
     CDS             1656..2582
                     /locus_tag="AC162G_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5075563.1"
                     /translation="MIRTEGLTKIFDGDKRTGKITAVDRLDLDVKEGEVFGFLGPNGA
                     GKTTTVRVLCTLIGPTSGRAWVNGIEVGTDDTAIRASVGILTEQPGMYDRLSAEYNLD
                     IYARLYGVSDPAGQVEKYLRMLGLWDRRRDPAGSFSKGMKQKLAIARAILHEPRTLFL
                     DEPTAALDPEAAKLVRDFIEELKTEGRTIFLCTHNLDEADRLCDRIAVFKHQIIKVDT
                     PGRMRASVFGRAVEFRLRRIDETMVAMVKRLDFVKSAEVGDGKLVVGLDDPESNNPIL
                     VKALVEAGAEVQFVAEVRHSLEDVYLSLIAEG"
     gene            2663..3448
                     /locus_tag="AC162G_06845"
     CDS             2663..3448
                     /locus_tag="AC162G_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5075564.1"
                     /translation="MRIRTIVRKEWAEVFKNKLVLMTVLALPLVFIAVPLFQLAITRN
                     VDAGELNDAPGNIAQLCTLQGLAPTECMQIYLSQSFMVFFLLMPLAIPVTIASYSIVG
                     EKTTHSLEPLLATPTSTAEIILGKALAAVIPAIAATWLSFAIFLVGARFFVVSDRVYE
                     VIANPMWLAGMLFVAPLLTVLSVNVAIVISSRVNDPRAAEQLGTLVMLPVLGLFFLQI
                     LGLVALNLTNMLLLGLILIAVDAVMVAVGVKLFQRETILTRWR"
BASE COUNT          650 a         1062 c         1107 g          649 t
ORIGIN      
        1 gcggatgggc aaatcatggt cgctggaggc gaagtcatca ttagcgggcg cgagacgctc
       61 agctcacttg aggtattcga tcccggcgac aatgcctggc gattggggcc ggacctgccg
      121 gcgccgattc acggcgtcgg cggcgcctca ctcggcggcc gcttctttct gcttggcggc
      181 tcgcgcgaag cgggtggaat cgccaattcg ggcacggtgc aaatctactt gccgtaggcg
      241 ctcacgcagg agaattcgat gaaacgacag gggcgattga ttgccctcgt gcttctctcc
      301 gctgtcttca acgcatgtgc agcgccggtt gccacgccag cggcgacatc ggttcgaacc
      361 gtatcaccac cagaagcgat cgcgctggca gatgagttct attcgacgct cgtggtcgag
      421 cgcgacttcg ccggcgcgac ggaaaacttt gacagtgtga tgctgagtgc gcttccggag
      481 gcgaagctcg aagaaacgtg ggcaacgatc gtcgctcaag tcggagctta tcaagagcgg
      541 ctggcagatc ccaccgtcga gcgcgtcgat cgatacgagc gcgtcacaat cgccttgaag
      601 ttcgagagag ccgttctgga catgcaggtc gtagtcgatt cgacgaccgg cctgatcagc
      661 ggcttattct tcgtgccaag ccggcaagcc tcgcttctgt acacgacgcc gggctacgcc
      721 gatcccgata cgttcgagga gcaggatgtc acggtcggca gtggcgagtg gacgctgccg
      781 ggaacactga cgatgcccaa aggtgaaggc cccttcccgg ccgtcgtgct cgtgcatggg
      841 tcgggtccca acgatcggga tgagacgctt gggccgaaca agccgttcaa agacctggcc
      901 tgggggctgg cttcccaggg tattgcagtc ttgcggtacg agaaacggac gaaagtctac
      961 gccgatcggc ttgtctccct ggacggcgag ttcaccgtca gggaagagac catcgacgac
     1021 gcattggccg cagcttcctt actgcgggag aggcaggaga tagaccccgg ccggatcgtc
     1081 gtgctcggcc acagcctggg cggcatgctc gcgccgagga tcgcgcaagc cggcccggac
     1141 gtcgcagggc tgatcgtcat ggccggcccg gcgcgcccgc tcgaagatct catcctggaa
     1201 caggcccgaa ctatcgtgtc gcaaagcggg aagccatcgc tacaagaaca gcagcaaatt
     1261 caggctatcg agcagcaggt gcaggcgata aaggcgttgc agccatcggg cgatggctcc
     1321 ggcaaggaga tgttgctggg cgcgccggcg tcgtattggc tcgacctgcg cggatacgat
     1381 cccgccgaag ccgccggcgt gctcacacag ccgatgctca ttctgcaagg cgagagggac
     1441 taccaggtct cgatggcgga cttccggcgc tggcaggatg tgctatcatc tcgaccgcgc
     1501 gtgcggttca agagctaccc ggagctgaac cacctgttca tcgcgggatc aggcccgagc
     1561 gcgccggaag aataccagac gccgggccat gtcgccgcgt atgtcatcga cgacctggcg
     1621 agctggatcg cgcagctacc gtaaggattc tccacatgat tcgaaccgaa ggactgacca
     1681 agatattcga cggagacaag cgcaccggca agatcaccgc cgtcgatcgc ctggacctcg
     1741 acgtgaagga aggcgaggta ttcggtttcc tcggtccgaa cggcgcaggc aagacgacga
     1801 cggtgcgcgt gttgtgcacg ctgatcggac cgacctcggg tcgcgcctgg gtgaacggca
     1861 tcgaagtggg gacggacgat acggccatcc gcgcctcggt cggcatcctg accgagcagc
     1921 ccggcatgta cgatcgcctg agcgcggagt acaacctcga catttacgcc cggctgtacg
     1981 gcgtatccga ccctgccggc caggttgaga agtatctgcg catgctggga ttgtgggatc
     2041 gccggcgcga tccggccggc tcgttctcca agggcatgaa gcagaagctc gccatcgcgc
     2101 gcgctatttt gcacgagccg aggacgttgt tcctggacga gccgaccgcc gcgctcgacc
     2161 cggaagccgc caagctcgtg cgcgatttca tcgaggagct gaaaacagaa ggccgaacca
     2221 tctttctgtg cacgcacaac ctggacgagg cggatcggtt gtgcgatcgg atcgccgtct
     2281 tcaagcacca gatcatcaag gtcgacacgc cgggccgtat gcgcgcgtcg gtgttcgggc
     2341 gcgccgtcga gttccggctg aggcgcatcg acgaaacgat ggtggccatg gtgaagcgcc
     2401 tcgatttcgt gaaaagcgcc gaggtcggcg acggcaagct cgtcgtcggg ctggacgatc
     2461 ccgagtcgaa taatccgatc ctggtgaagg cgcttgtcga agcgggcgcc gaggtgcaat
     2521 tcgtcgccga agttcgtcac tcgctcgaag acgtgtatct ctcgctgata gccgaaggct
     2581 gatggctgat agcttatcgc cgatggccta tcactgatcg caacacgcaa cacgcaacac
     2641 gtttcacgcc acaggagtcc tcatgcgcat acgaactatc gtccgcaaag agtgggcaga
     2701 ggtcttcaag aacaagctgg tgctgatgac cgtgctcgcc ctacccctgg tcttcatcgc
     2761 cgtgccactg ttccagttgg cgattactcg caacgtggac gccggcgagc tgaacgacgc
     2821 accgggcaac atcgcgcagt tgtgcacgct gcagggcctc gccccgacgg agtgcatgca
     2881 gatatacctc tcccagtcgt tcatggtgtt cttcttgctc atgccgctgg cgatcccggt
     2941 gacgatcgcc tcgtacagca tcgtcggcga gaagacgacg cactcgctcg agccgctgct
     3001 ggcgacgccg acctcgacgg ccgagatcat cctgggcaag gcgctggccg ccgtgatacc
     3061 ggcgattgcc gcgacctggc tttccttcgc gattttcctc gtcggcgccc ggttcttcgt
     3121 cgtcagcgac cgggtatacg aggtcatcgc caaccccatg tggctggccg gcatgctgtt
     3181 cgtggcgccg ctgttgacag tgctgtcggt caacgtcgcc atcgtcatct cctcgcgcgt
     3241 caacgatccg cgcgccgccg agcaactggg aaccctggtg atgctccccg tcctcggcct
     3301 gtttttcctt cagattcttg gcctggtggc gctcaacctg accaacatgc tcttgctagg
     3361 cctgatcctg atcgccgtcg acgccgtgat ggtggcggtc ggcgtcaagc tctttcagag
     3421 ggagacgata ctgacgcggt ggcgttgaat ggcctatcgt ttatcgct
//
LOCUS       DBNHXB010000173         5812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_207887, whole genome shotgun sequence.
ACCESSION   DBNHXB010000173 DBNHXB010000000
VERSION     DBNHXB010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_207887"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="AC162G_06850"
     CDS             <1..309
                     /locus_tag="AC162G_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007691559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5075565.1"
                     /translation="AGAAGVLYGLSFRQVQFLTGFLPGIKAFTAAVLGGIGNVPGAML
                     GGLFLGVIESIGPTLFLDGLNVPAPNQLKDVIAFTMLVLVLIFRPSGIMGERITVKKA
                     "
     gene            380..2077
                     /locus_tag="AC162G_06855"
     CDS             380..2077
                     /locus_tag="AC162G_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5075566.1"
                     /translation="MSEKTGHNWNAVIRMGLLGGVVAVYLCLVGLVGEFSGVALITGV
                     IAVGHTMLLLAGLGTGFLAARRAPAGQAVLAGAVAGLIAGAMLAALVIIGSLVNLRAV
                     MLQASPLLYNLLTFNLGVGGFWVPLIAGGVLGAVAGMIYGLPPRIRQLVTSALAALVG
                     LGLFAEQLRTPMLTGSLSWLARFLFGPEGLTPAGTLTTLVLVAGGVWVWQQRSPSVRQ
                     RISEMSDTERRTSRIALIGAGLIAVLLLPLALGKFFAQVMADVARYLLMGLGLNITLG
                     FAGLLDLGFVAFFAVGAYTVGLLTSTAEYGIAQWSWWAAAPFAVVLAMAFGAFLGLPI
                     LGMRGDYLAIATLGFGEIIRILAGSDLLKPILGGPRGILNIPKPIEVPPDHPLSGPNQ
                     IYYLALVCIVLAAFVAWRLRGSRLGRTWMAVREDEDVAEALGVNLVQIKLLAYMLGAA
                     FAGLGGAIFAGLVGSTFASSMQLLVSIYVVSLIIVGGMGSIPGVVVGAIFMIGLPGLL
                     REFEDYLFLFYGAALIAMMLYKPEGLWPSSVIRRELRVEAESEMEPSDAAASGAAAP"
     gene            2094..2924
                     /locus_tag="AC162G_06860"
     CDS             2094..2924
                     /locus_tag="AC162G_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5075567.1"
                     /translation="MPILTSHRVTKRFGGLVAVSTLDFEIEKGAIASVIGPNGAGKTT
                     FFNCITGFYRVDEGEIRFDGTPIHHLRSVKIAHLGISRTYQNIRLFANMTALENILLG
                     QHYHLHSSWLGAVVGKRSTQEEEDRAHEEARRLLRFVGMQGKGDTLARNLPYGDQRRL
                     EIARALANNPKLLLLDEPTAGMNPSETQHMMDFIRRLRDELDITILLIEHQMRVVMGI
                     SERITVLDYGEKIAEGTPVEIQNNPKVIEAYLGRGSAVAAPARTAPPSISATPASATG
                     "
     gene            2930..3643
                     /locus_tag="AC162G_06865"
     CDS             2930..3643
                     /locus_tag="AC162G_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5075568.1"
                     /translation="MAILEVENVHTYYGHIHALKGISLTVEQGEIVTLIGANGAGKST
                     TLNTISGLLRPREGHIRLAGEDLSKYPAHEIVAKGVVQVPEGRRMFTRLTVTENLEMG
                     AYTLSDGHSIRENLDRVFTLFPRLKERRHQVAGTLSGGEQQMLSTGRALMANPKVLLM
                     DEPSMGLAPVLVDSIFDTIKRLHEEGTTILLVEQNARMALQVANRGYVLEAGQVVLSD
                     KAEGLRQNEQVQKVYLGIA"
     gene            4045..4722
                     /locus_tag="AC162G_06870"
     CDS             4045..4722
                     /locus_tag="AC162G_06870"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR5075569.1"
                     /translation="MAMVFVPGGEFRMGSGDADPGAEDDEKPQHDVVLDAFWIDRTEV
                     TNAMYARCIQAGTCTAPMRSSRYDVATHADHPVTGVTWFQAQEYCAWAGRRLPSEAEW
                     EKAARGPDGRLFPWGDGAPGPSLLNFDGQVDDTTTVGSYPAGASPYGALDVAGNVWEW
                     TADWYAEGYYADSPRTNPPGPASGNRRVLRGGAWGADAQAVRAANRFWAFPGRNDFDG
                     FRCARSH"
     gene            complement(4861..5751)
                     /locus_tag="AC162G_06875"
     CDS             complement(4861..5751)
                     /locus_tag="AC162G_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075570.1"
                     /translation="MLIATYRDDQNPAADSPTYTLIDLASCDASGCASKVISVSDLPV
                     WSPNGSQMLVGDFGRATIFHGNADGQSLAKLADGQYPFWLDNQTYGYVRWSGTQATAE
                     LEIVATAIGDDEIEVMLRKADLQAALPESERSGWSIVRIGPVTISPTNPDLLVIAAYA
                     KHERLQPTWPGRSYVFLFDRRSGVISLRLQSDVGSWFNFTDFSPDSRWLITSSSDFAR
                     FTWSLILHDIGRSETRTLTSLSQAGYLHAFPEYEWSSNGQWLLVLDDGVLWLIAPDHD
                     YQRTIVPQSPGCTLAAWVNT"
BASE COUNT         1065 a         1727 c         1899 g         1121 t
ORIGIN      
        1 gccggcgcag ccggtgttct gtacggactg agcttccgcc aggtgcaatt cctcaccggg
       61 ttcttgccgg gcatcaaggc cttcaccgcc gccgtgctgg gcggcatcgg caacgttccc
      121 ggcgcgatgc tcggcggact cttcctgggc gtcatcgaat ccattgggcc aacgctgttc
      181 ctagacgggc tgaacgtgcc tgcgccgaat cagctcaaag acgtgatcgc cttcaccatg
      241 ctggtgttgg tgctgatctt ccgtccatcg ggcattatgg gcgagcgcat cactgtcaag
      301 aaggcctaga agagatagta gtaacgactt cagtcgttac acaggcgatt caagttgcta
      361 ctacagcaag aggatgacca tgagcgagaa aactggtcac aactggaacg ctgtcatacg
      421 catgggcctg ctgggaggtg tcgtcgcggt gtacctctgt ctggtcggct tggtggggga
      481 gttcagtggg gtagcgctga tcacgggcgt cattgcggtg gggcacacca tgttgctgct
      541 ggccgggctg ggcaccggtt tcctggccgc gcgtcgtgcg ccggctggcc aggcggtgtt
      601 ggcaggtgct gtggccggcc tgatcgccgg ggctatgctg gcggcgctgg tgatcatcgg
      661 cagcctggtg aatctgcggg ccgtgatgct gcaggcttcg ccgcttttgt acaacctgtt
      721 gaccttcaac ttgggagtgg ggggattttg ggtgccgctg atcgcggggg gtgtgcttgg
      781 agcggtggcc ggcatgatct acgggttacc tccgaggatt cgccaactcg tgacctcggc
      841 gctggctgcg ctggtcgggc tggggctgtt cgcggagcag ctgcgcacgc cgatgctgac
      901 cggcagcctg tcctggctgg cccgcttcct cttcgggcct gaaggcctga caccggccgg
      961 gacattgacc acgctcgtgc tggtcgctgg cggcgtgtgg gtctggcagc agcgcagccc
     1021 gtccgtgcgc cagcgcattt ccgaaatgtc agataccgag cgccggactt cccggatcgc
     1081 attgatcggc gcgggcctga tcgccgtcct gctccttccc ctggcgctgg gtaaattctt
     1141 cgcccaggtg atggccgatg ttgcgcggta cttgctgatg gggctggggc tgaacatcac
     1201 gctcggcttt gccggcctgc tcgacctggg ctttgtggct ttcttcgccg ttggcgctta
     1261 caccgtcggc ttgctgacct cgacggccga gtatggcatt gcccagtggt cgtggtgggc
     1321 ggccgcgccc ttcgcggtcg tgctggctat ggcgttcggg gcgttcctgg gcctgccgat
     1381 cctcggcatg cgcggcgact atctcgccat cgccacgctg ggctttggcg agatcattcg
     1441 gatactggcc ggttcggacc tgctgaagcc gatactgggc gggccgcgcg gcatcttgaa
     1501 catccccaaa ccgatcgagg tgccccccga tcatccacta tccgggccga accagatcta
     1561 ctacctggcc ctggtgtgca ttgtgctggc cgcctttgtc gcctggcgct tgcgcggttc
     1621 gcgcctgggc cggacgtgga tggcggttcg cgaagatgaa gacgtggcgg aagcgctggg
     1681 cgtcaatctg gtgcagatca agctgctcgc ctacatgttg ggcgcggcct tcgccgggct
     1741 gggcggcgcg atcttcgccg ggctggtcgg atcgaccttt gccagcagca tgcaactgtt
     1801 ggtctcgatc tacgtcgtct cgctgatcat cgtcggcggg atgggcagca tccccggcgt
     1861 ggtcgtgggc gcgatcttta tgattggcct gccgggtctc ctccgagagt tcgaggatta
     1921 tctcttcctg ttttacgggg cggccttgat tgcgatgatg ctgtacaaac cggaagggct
     1981 gtggccctct agcgtcatcc ggcgtgaact gcgcgtcgaa gcagagagcg agatggagcc
     2041 atcggatgcc gctgccagtg gtgccgcagc gccgtaaggg atacggagac atcatgccga
     2101 ttctaacctc acatcgggtc actaaacgct tcggcggcct cgtcgcggtc agcacgctgg
     2161 atttcgagat cgagaaggga gcaattgcca gcgtgatcgg ccctaatggc gccggcaaga
     2221 cgaccttctt taactgtatc actggcttct accgcgtcga tgagggtgaa attcgcttcg
     2281 acggcacgcc aattcaccat ctgcgatcgg ttaagattgc acatctgggt atctcgcgca
     2341 cgtaccagaa catccggctc ttcgccaaca tgaccgcgct ggagaacatc ctgctcggcc
     2401 agcattacca cctccattcg agctggctgg gagcggtggt gggcaagcgc agcacgcagg
     2461 aggaagaaga tcgcgctcac gaagaagcgc ggcggctgct gcggtttgtc ggaatgcagg
     2521 gcaagggaga taccctggct cgcaacctgc cgtacggcga ccagcggcgc ctggaaatcg
     2581 cccgcgcgct ggcgaacaat cccaaactgc tgctgctaga tgaaccgacc gccggcatga
     2641 acccatccga gacccagcac atgatggact tcatccgccg gctgcgtgac gaactggata
     2701 tcacgattct gctgatagag caccagatgc gcgtggtgat gggcatctcg gagagaatca
     2761 ccgtgctgga ttacggcgag aaaatcgccg aagggacgcc ggtcgaaatc cagaacaatc
     2821 ccaaggtgat cgaagcctat ctcgggcgcg gcagcgccgt agcagcgccc gctcggactg
     2881 ccccgccttc catttcagcg actccagcct ccgcgacagg gtgatcgcca tggctatctt
     2941 ggaggtcgaa aacgtccaca cgtattacgg gcacatccac gcccttaagg gcatttcgct
     3001 caccgtcgag cagggcgaga tcgtcacgct catcggcgcg aacggtgccg gcaagagcac
     3061 cacgctcaac acgatcagtg ggctgctgcg gccgcgggaa ggccacatcc gcctggcggg
     3121 cgaggacttg agcaagtacc cggctcacga gatcgtcgcc aaaggcgtcg tccaggtgcc
     3181 ggaaggccgc cgcatgttca cccggctcac cgtcaccgaa aacctggaga tgggcgctta
     3241 cacgctttcc gacgggcata gcatccgtga aaacctcgat cgtgtgttca cgctatttcc
     3301 caggctcaag gaaaggcggc atcaggttgc cggcaccctc agcggcggcg agcagcagat
     3361 gctttccacg ggccgtgccc tgatggcgaa tcccaaagtg ctgctgatgg atgagccgtc
     3421 gatgggtctt gcgccggtgc tggtcgacag catcttcgat acgatcaagc gcctgcacga
     3481 ggagggcacg acgatcttgc tcgtcgagca gaacgcgcgc atggcgctgc aggtcgctaa
     3541 tcgagggtac gtgctggagg ccgggcaagt cgtgttgagc gacaaggcgg agggcctgcg
     3601 ccagaacgag caggtgcaga aggtttatct gggcattgcc tgatcgggcg ggaagtgagg
     3661 gaactgaagg aactgaagga actcagggtg cagaaggctt atctgggcat cgcctgatca
     3721 ggttgctggt ggtgtgttct gttcattcgg atacgctgcg gtcccaaatc cgcacacaag
     3781 gccagtttct gcgaaactgg ccttcgccgt gataacaggc actgcttgat gccatatcgg
     3841 caagtcacac ccgttcttgg accggaagca cgtcaatatt ccgagcgccg gagtcgaggc
     3901 ttcagccgga ttccgcctaa aggctcgact ccaatcggaa ttctcaagtg cgggcagtat
     3961 gttttggcgt tgctgctgct ggcttcggcc tgccaggccc ccgcgccaac tgccggttcg
     4021 acgatggtct cggaagtaga cagtatggcc atggtgtttg tgccaggcgg ggaatttcgg
     4081 atgggcagcg gtgatgccga tccaggtgcc gaagacgacg aaaagccgca gcatgacgtt
     4141 gtccttgatg ccttctggat cgaccggaca gaagtgacca acgcgatgta tgcgcgatgc
     4201 atccaggccg gaacatgcac agcaccaatg cgctcgtcgc gttacgacgt cgcgacccac
     4261 gccgaccacc ctgtcacggg agtgacctgg tttcaggcgc aagaatactg cgcgtgggcc
     4321 gggcgcaggc tgccgtccga agccgagtgg gagaaggcgg cacgcgggcc tgatgggcgt
     4381 ctgtttccgt ggggtgacgg ggcgcccggc ccgagcttgc tgaacttcga cgggcaggtc
     4441 gacgacacaa ccacggtggg cagctacccc gccggcgcca gtccctacgg cgcgctggac
     4501 gtggccggca atgtatggga atggacggcc gactggtacg ccgagggcta ttacgccgat
     4561 tcaccacgca cgaatccgcc gggaccagcc tccggcaata gacgcgtgct gcggggcggc
     4621 gcctggggcg ccgacgccca ggccgtgcgc gccgcgaatc gcttctgggc atttcccggc
     4681 cgcaacgact tcgacggctt ccgatgcgcc cgctcacact gagccgcaga tgagagccga
     4741 tcccgttgct ccggccgggt ctgtgacccg accgatccgg ggccgacgat cagtacgaag
     4801 cctccccgtg ggacagaccg ccgacgagca gatctgggca tcgcctagcg caaccatcgt
     4861 tcatgtgtta acccacgccg cgagtgtgca gccgggcgac tggggaacga tcgtgcgctg
     4921 ataatcatgg tccggcgcga tcagccagag gacgccgtcg tcgaggacga ggagccactg
     4981 cccgttggat gaccattcgt attcagggaa agcgtgcaga tagccggcct gtgaaagaga
     5041 agtcaaggtc cgggtttcac tccgaccaat atcgtgcaag atgagtgacc atgtgaaacg
     5101 ggcgaagtcc gagctgctcg ttattaacca acgactgtcg ggcgagaagt cggtgaagtt
     5161 aaaccaactg ccgacgtcgg attgcagccg cagggagatc acgcccgaac gccgatcgaa
     5221 taagaagacg tagcttctgc ccggccaagt gggttgtagg cgctcatgtt tggcataagc
     5281 cgcgatcacg agcaagtccg ggttggttgg gctaatggtc accgggccaa tgcggacgat
     5341 cgaccaaccc gaacgctcgc tttccggcag cgccgcctgg agatccgcct ttcgcagcat
     5401 cacctcgatc tcgtcatctc caatcgcagt cgcgacgatc tccaactcgg cggttgcctg
     5461 cgtgccactc cagcgaacat agccataggt ctgattgtcc agccaaaatg gatattgacc
     5521 gtctgcaagc ttcgccagcg attgaccgtc cgcattcccg tgaaatatgg tggcgcggcc
     5581 gaaatccccc acgagcattt gtgatccatt cggagaccag acaggaagat cggaaacaga
     5641 gatcaccttc gacgcgcagc cggacgcatc gcagcttgcc agatcgatta gcgtgtacgt
     5701 ggggctatcg gcggccgggt tttgatcgtc acggtaggtg gcgatgagca cgctgtgcgg
     5761 cggaggcccg atcaaagggg aataggagac cagcccggaa gtgaccggcc cc
//
LOCUS       DBNHXB010000174         2905 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4569730, whole genome shotgun sequence.
ACCESSION   DBNHXB010000174 DBNHXB010000000
VERSION     DBNHXB010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4569730"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1789
                     /locus_tag="AC162G_06880"
     CDS             <1..1789
                     /locus_tag="AC162G_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5075571.1"
                     /translation="DEALIANAQAAMWIDSLINLQWRRSILGPSIGLAPFPISADGST
                     SHTTPMTPVCVAMSSGTAHPQEAWAWLSFLTMQSMTSAPDAVSARPSVAEASDFWTDL
                     TDETRLVLRFALEHAWYGSQHPEALRTARGAFVQALTGETDLTSALDSMTLPIEATPL
                     PATTPVVVAAPRPTPEAGGIVTVDYYASGFVHPSPNAIEALAQQFNRAHPGIEARPSD
                     DLAVPAYHVFTVADVAKQYDCLAWSNASGEALDQFYSLDPLLAAEDQSLIAGFDPALL
                     DALRVNGKLYALPATSLPMVIYYNADHLDKMGLQPPDPDWTVDDFVSLAAAATSGETE
                     NKTYGFVPFQADSIGFLFAAQGVELYDLSTEPPTIHFDDPDTARAAQWMVDLIHSGIM
                     SPLTGDLFHATAERLVVSGHAAMWSDFAGLRGGFVQDEPADFKVGVAPPPVAPARLPP
                     PLVYGFYISRRVEEPSACWSWIKFLSEQPSAFIGVPARRSVTESKAWEAAVGVDTAEA
                     YRAAVSRPVSSQHPDWRVANWLEQALAASLEGEEPAAALADAQHKVDTFLACLAAAPG
                     DDSDQRDACARQADPEFKTLQELLDEDAP"
     gene            1857..>2905
                     /locus_tag="AC162G_06885"
     CDS             1857..>2905
                     /locus_tag="AC162G_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR5075572.1"
                     /translation="MKRRYPTWFRMARAPGFAILAILLLTACGSGSQSVEPTPTPFPT
                     PIVPEKPVYTVARGTVTKTLEFTGRVTPVEQQELFFRTDGFVNAVYVERDARVTEGDV
                     LAELDLGELENQLAQAQLDLLKAERQQADALAEAQSKLDSARTRLQQAQAKDVQAEVI
                     VREIAVIRAREALAHAQDEYNKAVNRPWETDDSMRAYKQAVTDAERNLEIAEAEYNAA
                     LAAQKSHAFDVELLKQEVALAERQLARLTGDEDTAGDLNVEQARLDIKKIEDQIAAAR
                     LIAPFDGTVLSLNVRAGSRAEAFRAVAVVGDPGTLEITADLEAGQVSELSVDQPAIVR
                     LRSRPEEDLAGRVRQL"
BASE COUNT          560 a          914 c          931 g          500 t
ORIGIN      
        1 cgacgaggct ctgattgcga acgcgcaggc ggcgatgtgg atcgacagcc tgatcaactt
       61 gcagtggcgg cgcagcatcc tggggccgtc gattgggctt gcgccgtttc cgatttcggc
      121 ggatggatcg acctcgcaca cgacgccgat gacgcccgta tgcgtggcga tgagcagcgg
      181 cacggcgcat ccacaagaag cgtgggcctg gctaagtttc ctgacgatgc aatcgatgac
      241 gagtgcgccg gatgccgtat cggcgcgccc ctcggtcgcg gaagccagtg atttctggac
      301 cgacctgacc gatgaaacgc gcttggtatt gcgcttcgcg ttagaacatg cctggtatgg
      361 cagtcaacat cccgaagcct tgcggaccgc gcgcggcgca ttcgtccagg ccttgacggg
      421 cgagaccgat ctgacctcgg cgttagacag catgacactg cccatcgaag ccaccccgct
      481 gcccgcgacc acgccggtcg tcgtagcagc accgcgtccg acaccagagg ccggcggcat
      541 cgttacagtg gattactacg cgagcggctt cgtccacccc agtccgaatg caatcgaagc
      601 gttggcccag caattcaatc gcgcgcatcc cggcatcgag gccaggccat ccgacgactt
      661 ggccgtgccc gcataccacg tgttcactgt cgctgacgtg gcaaagcaat acgattgtct
      721 tgcctggagt aatgcctctg gcgaagcgct cgatcaattc tacagcctgg acccgctgct
      781 ggcggccgaa gaccaatcgc tcatcgctgg cttcgatccg gcgcttctgg atgctctgcg
      841 tgtcaatggc aagctgtacg cactgccggc taccagcctg ccgatggtca tttactacaa
      901 cgccgaccac ctggacaaaa tgggccttca gccgcccgat cccgattgga cggtggatga
      961 ttttgtgtca ctggccgcgg cggcgacatc aggagaaaca gagaacaaga cgtatggctt
     1021 cgttcccttc caggcggatt ccatcggctt cttgttcgcg gcgcagggtg tggaactgta
     1081 cgatttgagc accgaaccgc cgacgataca ctttgacgat cccgatacgg cccgcgcggc
     1141 acaatggatg gttgatctca tccattcagg aatcatgtct cccttgaccg gcgatctctt
     1201 ccacgcaacg gcagaaagac tggtcgtatc cggtcatgcg gcgatgtggt cggacttcgc
     1261 cggactgcgg ggtggcttcg tacaggacga accggcggat ttcaaagtcg gcgtcgcgcc
     1321 gccgcccgtc gcccccgctc gcttgccacc gccgcttgtg tacggcttct atatctcgcg
     1381 ccgcgttgag gaaccaagtg cgtgttggtc ctggatcaag ttcctgtccg agcagccatc
     1441 ggccttcatc ggcgtgccgg cgcggcgatc cgtaaccgag tcgaaagcat gggaagcggc
     1501 ggtgggagtg gatacggcag aggcttatcg tgcggccgta tcgcggcctg tgtcttccca
     1561 gcatccagac tggcgcgtcg ccaactggct ggagcaggca ctggcggcat cgctggaagg
     1621 tgaggagccg gctgcggcat tggccgacgc tcagcacaag gtggatacct tcctggcctg
     1681 cctggccgct gctccgggtg acgattcgga tcagcgcgat gcctgcgcca ggcaagccga
     1741 cccggaattc aaaacgctgc aagagttact cgacgaagac gcgccctaga cacaaacgtg
     1801 cgacgcattt ccaaagtgcg tcgcacgtgg ctgtgaggtc gttcagcgag gtgacgatga
     1861 agcgaagata cccaacttgg ttcaggatgg cgcgagcgcc tgggttcgcc atcctcgcaa
     1921 tcttgctgct caccgcctgc ggctcaggca gccaaagcgt agagccgacg ccgacgcctt
     1981 tccccacgcc aatcgtgccg gagaagccgg tctacaccgt ggcgcgcggc acggtgacca
     2041 agacgctcga attcaccggg cgcgtcacgc cggtcgagca gcaggagttg ttcttccgca
     2101 ccgacggctt cgtgaacgcc gtgtacgtcg agcgcgatgc gcgcgtgacc gaaggcgacg
     2161 tgctggccga gttggacctg ggcgagctgg aaaaccaact ggcgcaagcc cagctcgacc
     2221 tgctcaaggc cgagcgccag caggccgacg cactcgccga ggctcaatcc aaactggata
     2281 gcgcgcgcac tcgcttgcaa caggcgcagg ctaaagatgt gcaggctgag gtgatcgtca
     2341 gggagatcgc cgtcatccgt gcgcgggagg cgctggctca cgcgcaggac gagtacaaca
     2401 aggcggtcaa tcgcccctgg gagaccgacg actcgatgag ggcctataag caagccgtca
     2461 ccgacgccga gcgcaacctg gagatcgccg aggcagagta caacgcggcg ctggccgcgc
     2521 agaagtcgca cgccttcgac gtcgaattgc tcaagcagga agtggcattg gccgagcgcc
     2581 agctcgcccg gctgacgggc gacgaagaca cggcaggcga tctgaacgtg gagcaagccc
     2641 ggctcgacat caaaaagatc gaagaccaga tcgcggcggc gcggctgatc gccccgttcg
     2701 atgggacagt gctgtcgttg aacgtccgcg ccggtagccg tgccgaagcg ttccgggcgg
     2761 tggccgtggt gggcgatccc ggcacgttgg agatcaccgc cgatctcgaa gccggccagg
     2821 tgagcgaact cagcgtcgac cagccggcga tcgtccgctt gcgcagccgg cccgaagaag
     2881 acctggccgg ccgggtgcga caact
//
LOCUS       DBNHXB010000175        25363 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3227880, whole genome shotgun sequence.
ACCESSION   DBNHXB010000175 DBNHXB010000000
VERSION     DBNHXB010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 25363)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 25363)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25363
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3227880"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /gene="udk"
                     /locus_tag="AC162G_06890"
     CDS             complement(<1..560)
                     /gene="udk"
                     /locus_tag="AC162G_06890"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HIR5075573.1"
                     /translation="MPAPRIVIGVSGGTGSGKTTVVRAIVGRIGADRLTFIQQDSYYR
                     DWDDLPPAAREHRNFDHPDSLETELLIEHLKRLRAGQPIDMPVYDFRNHSRTSETHRV
                     EPKPVILLEGILIFVEKPLRDLIDVKIFVDTDADLRFIRRLQRDMAERGRSMDSVIHQ
                     YLDTVRPMHLEFVEPSKRYADLIIPEG"
     gene            complement(607..1248)
                     /locus_tag="AC162G_06895"
     CDS             complement(607..1248)
                     /locus_tag="AC162G_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075574.1"
                     /translation="MMLEAIVGIAAGTLIAAALAGIRRVRNWSKAGRIAALVSLLAGL
                     LTALSPAWQPAAGALPLAVYASVFIAAGGLILAILEKAPPWMLLATSALLIYAARLPG
                     EAAVPISIAVLANFAAGVETLPGLDAAARSWRGSVDRTRIALWLGISIALAASAAESL
                     LERGAWLGSAPRDAWLMAAWITSSGSLLVEHSRWRAALMFAAALCIGLSALSL"
     gene            complement(1245..2513)
                     /locus_tag="AC162G_06900"
     CDS             complement(1245..2513)
                     /locus_tag="AC162G_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075575.1"
                     /translation="MTDLPANVIVRRNAWQSAWRIATGDVFFAAALLGLAFLLGSAAL
                     IAQAPRGDPAAYARWLSEAHLRFGAAADALATLGLFDLVHSIFFRGLAALLGFALIAR
                     LADRIAAMRAASRPVPPPDSPARTFDSGEPAEEITRRMRHYRVCSTESYLLAHRFPLA
                     YIAAMAAYAGSLIMLTGMATASLGDWRIDGLSVTPGVPARIEDTALTFLAAEIDPGGE
                     VAVRLLEGESEVAQGIAAPGKPAIQSAASIYVRDVLPALRVTGEDGEGRALQLQTSVQ
                     GEASSEVLLLFDANQPDKFFAAPAAKLAVGVSLLESDPAPRYRVSILSSSSAESIADE
                     TIVPGQSMAANGYRLAFANESHAAIDVVRAPFQFVVAIGFVVAVAGLGFAALYPARRI
                     WTVPGEGGARLVSDDAEFDLSTLAAGRSVR"
     gene            complement(2554..2626)
                     /locus_tag="AC162G_06905"
     tRNA            complement(2554..2626)
                     /locus_tag="AC162G_06905"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2591..2593),aa:Thr,seq:cgt)
     gene            complement(2803..4002)
                     /locus_tag="AC162G_06910"
     CDS             complement(2803..4002)
                     /locus_tag="AC162G_06910"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5075576.1"
                     /translation="MPHTTELETRLARFRRVLELSRILNSTLDLVTLLTLIIEAAREI
                     TRTEASSIMLTDPKTGDLYFEAATGTKSEEVKRVVVPPNSLAGWVAREGKPQIIDDVS
                     SDPRWSPLPDQQSGFKTRALIALPLQVKGRTIGVLESLNRVDNTPFDDEDIDVLSALA
                     AHAAVAIENARLFQQSDLISEMVHELRTPLTSIVAYGELLMRDDLKREQQRSFVETIL
                     QESTRLTAMINDFLDLARLQSGRARLAHVPVDIAGLLRDCVSVMKPQADKKNIQVEVD
                     APAGLPPVEGDAGRLKQVLMNLLSNAIKYTAPGGRVNARAGQRNDSVQVAIEDTGRGI
                     PEQDLPHIFDKFYRVADSEGWATGTGLGLSITKEIVEVHGGQIDVVSQVGVGTTVTLV
                     IPRSYRA"
     gene            complement(4023..4784)
                     /locus_tag="AC162G_06915"
     CDS             complement(4023..4784)
                     /locus_tag="AC162G_06915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR5075577.1"
                     /translation="MQPLVERAARMLRAARHAVALTGAGVSTPSGIPDFRSADVGLWE
                     TSDPMEVASIYAFRENPDRFYRWLRPLATLMRDAQPNAAHFALAQLEHAGIIRTVISQ
                     NIDGLHPKAGSMHVAEVHGHVREAVCLACYRVTPSESYLARFITDGETPLCPACGGVL
                     KPNATLFGEALPAQALLRAMAAARQCDVMLVAGSSLEVAPAADLPHLALEHQARLMII
                     NFGETYLDDRADVLIRGDVADVLPRIVEAIDARRS"
     gene            complement(4830..5987)
                     /locus_tag="AC162G_06920"
     CDS             complement(4830..5987)
                     /locus_tag="AC162G_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE/TadZ family protein"
                     /protein_id="HIR5075578.1"
                     /translation="MAEKILIVDDDLESLKLIGLMLQRRGYQIAAAQGGAQALSKAES
                     EQPDLIILDIMMPDMDGYEVTRRMRANNKTADIPIIMFTAKTLVGDKVAGFQAGADDY
                     LTKPIHPAELSSRVEAVLLRRARTRAEMSVRPHARILGFIGAKGGVGTTTLAINVSVA
                     LAQGGKDDASQHVILSDLHSAAGDVGLQLGITRASGLQALVSRQADDVSAKLVESVLT
                     GHVSGMRMLLSPIEQRAETTDLPARHADAIIRQLASLAEVVVLDLGSRLDDGARAAVR
                     LCSRLIVVLEPQRPAVLLAQSMLAQLEKAGIGADKVMPVVVHRSPSAATLSKAVIEGI
                     LNRDLAVVVTPVPEAAFQASEQGAPLIMVQPGNIVSDQFRELARILMLGPI"
     gene            6214..9042
                     /locus_tag="AC162G_06925"
     CDS             6214..9042
                     /locus_tag="AC162G_06925"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5075579.1"
                     /translation="MPNAGLSNWSPLLILAAAAALAALAFLVRRRVLSRRELERRSSE
                     LDALVDFGRSIAQSRLDVDALCELIHRKASQIVDTSSFQIGLFEDDQYAIRLWIREGE
                     RLPPAKFDLAQNGGLIGWLRQSKQPLLIHDFEREIDALPARPRYLSNQPPRSAVFVPI
                     IAGEEAVGALSIQNDRPNVFDDDHVRLLSIVANQAAPVIANARLLEAERRRATQLALI
                     GEVSRQVAAILEIDVLFDRVVELIQSHFNYYFVAVALREEDSDELVLAGTTAKDARGR
                     RLKPGQGIIGAVVQSGEPLLVNDVLADSHYVALAFLPETRSELAVPLTFGQRILGALD
                     VESSELNAFTQEDLFILRTLADQVAIATHEARLYAAEREQAWVSTALLQVAEATTQAT
                     SVEEVLETVVRITPMLSGVDRCGILLWDATGSVFRAAASFGLDERAPDFDALTLGADD
                     WPDLFVAEATGEALQLDYAPGRLREAFGPGPLLALPLYARGERAGVMLVGSPEGSQLA
                     KRKAALIAGIANQAALAIESSQLLASQREEAWVNMALLQVAEAVGSQTELRDILTTVV
                     RLTPLLVGVETCLILLYDPASASFVAGDVYGIPRERLKLFADLRVPQAAWSLGEAPAD
                     IPLEVVERLQLSAPLAWPLVAKGDVVGVMVIGGGTEARAPSARHTNILAGIASQTAMA
                     VMNAQLADEAVARQRLEQELRVARKIQESFLPERCPSLPGWQIAAFWRSARQVGGDFY
                     DFIPLRDGTDRLGIAIADTADKGVPAALFMALSRTLLRAAAIGGRGAAEALTRTNDLI
                     LADARSDLFVTIFYIIVDASSEMVRFANAGHNPTLLVRAVSPIPEYLTEHGMALGVMP
                     DVTFEEQSLAVGQGDVVVLYTDGVTEARNEAGEEFGLERLERCVIDNMSGSVDDIVQA
                     IKDALRAHVGDEPPFDDITFVVLKRVNE"
     gene            complement(9141..10679)
                     /locus_tag="AC162G_06930"
     CDS             complement(9141..10679)
                     /locus_tag="AC162G_06930"
                     /inference="COORDINATES: protein motif:HMM:NF018905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase-like domain-containing
                     protein"
                     /protein_id="HIR5075580.1"
                     /translation="MQLYTEIVISETEAVPGEWQIAASLNVVGVHMALLNTGKVLLFS
                     ADMHDYAAINRGQSALYDPVNGDVTYPDLSRNLFCSGHCILPDGRLLAAGGQSCAQVP
                     WQVIISLLGFGRGADHDVHTFDPVTETWSRHADMPRARWYPTCVTLPNGKALIVSGYW
                     HHCIAPMAERLTGSDRFAMNATCEIFDPETSTLSAPQPFLQDIGLYPFMHVLPGGFLF
                     VHSGNKTRLFDLNRQEWLPQVFTMNSPGTRTYPGQGSSVLLPIRPDDPYVRVLIVGGS
                     TALEPGEDTPAIDRVEIFELDIQHPHESRWRETAPLAKPRILVDAPLLPDGSVLVING
                     AERGVADHNHGEVREAERFDPVTETWQRLAQAEVARLYHSTAVLLPDGRVLTAGSTGH
                     EWPPDRNELRLEVFSPPYVARGPRPVIDWAPSQVTYGEKFDVETRHGQSITSVALIRP
                     TTTTHNNNMDQRYVGLKIDEKSPWQLTVAAPPDPSIAPPGDYMLFIVNSDGVPSTAKF
                     IRVH"
     gene            complement(10775..12421)
                     /locus_tag="AC162G_06935"
     CDS             complement(10775..12421)
                     /locus_tag="AC162G_06935"
                     /EC_number="1.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF018905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase early set domain-containing
                     protein"
                     /protein_id="HIR5075581.1"
                     /translation="MSSWGSVNHAQKPCVLSFIVAKRRVCSVRRRSVMPDRLEIVKSK
                     IDASVHGKWDVAVANLGVVGVHMALLNTGKVLLFSADHHDYEAMDKGKAALWDPATDS
                     VTNSDLSRHLLCSGHCMLPDGRVLVVGGQAYGQNLRQFLRALAGRGRGVDHDIHTFDP
                     VTEKWSRHTDLPRARWYPTCVTLPDGKAMLVCGNWNRIVAQIVERLTGSDRFGINDTF
                     EIFDPETSTLTAPQPFLKDIGVYLFVHVLPGGFLYVHSWNKTRLFDLNKQAWIPQVFT
                     MNSPGTRIYPAPGTSVLLPISPGNAQPARVLIVGGSTALKPTEETPATDRVEIFELNA
                     EDPRQSRWRETAPLTHRRFVSETLLLPDGTILVIGGAKQGTGDHNHGAVLEPELFDPA
                     TETWQTLARASIQRLYHSTAALLPDGRVVAAGSTGHEWPPDEQELRLEVFSPPYLANG
                     PRPVVDWAPPQVSYGASFNIETGDARSIQSVAFVRPSTTTHNNNMDQRYVGLQIECRN
                     AAQLTVMAPPDGSVAPPGYYMLFIVNTDGVPSIAKFVRVQ"
     gene            12734..14215
                     /locus_tag="AC162G_06940"
     CDS             12734..14215
                     /locus_tag="AC162G_06940"
                     /inference="COORDINATES: protein motif:HMM:NF015963.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HIR5075582.1"
                     /translation="MKFETRRTWKNIAKNQVAEPLRIYKPDSLEQIIEIIREAERRRV
                     RVRAIGSGHSWSDVGLTTGFLVKPDRLNRVVPLDTSLLNGDVDTSTLFHIESGMRIRE
                     LNEELDRRGLALSNMGGYDGQTYVGAMSTSTHGSGLEFGPLPDFVETVEIVSDGGVVY
                     RIEPTNGITNRSAYEKKYPDKKVGKLIQDDDWFNAVVVGMGCMGIIYSVILRVAEKYW
                     LKEVRTLSTWGKVKEDLIKGRVLADNRHYEVLVNPYKVGGAHRCLITTRNPTDPPRNK
                     PHDKLNRNYLIELLATLSFIRRVLNLVLDLLPEITPELMNSAMEGLVDDEYTNVSYKV
                     LNIGTANQVTAYSAEIGFPLKENKYIDAVDKFLDMAEELKSVGVVFLSSPISLRFVNA
                     SSAFLSPQYGYDTCMVEIIALKGTQGYFEMIEQFENVLYGFSGRPHWGQVNSLTASHN
                     LIDSMYPQYHKWIDVHRQLNKNGTFDSSFSKRVGFTEHTFLPS"
     gene            complement(14289..15974)
                     /locus_tag="AC162G_06945"
     CDS             complement(14289..15974)
                     /locus_tag="AC162G_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HIR5075583.1"
                     /translation="MAETHFDAVIVGSGFGGSVTAYRLAEAGLRVCVLERGKAYPPNS
                     FPRSPYRMRRNFWDPSEGLYGLFNIWRFRNLEAVVSSGLGGGSLIYANVLIRKDEKWF
                     VKEDIHKGGYEYWPVTRADLDPHYDQVEKMLNAQRYPFEHAPYDQTAKTREFKAAAEK
                     LNLEWQLPNLAITFSNPGETPLPGEPIKEAHPNLHRRTRYTCRLCGECDIGCNYGSKN
                     TLDYNYLSEARRLGADIRTLCEVRSFEPRVGGGYVVRYVEHDPAAREGRKFATSQLPA
                     ITLTADRLILAAGTLGTTFLLLKNRNAFPHLSKQLGTRFCGNGDFLTFAIHSRQVKDG
                     KLVPRFLDAGYGPAITSAIRIPDVLDGSQMRGHYIEDAGYPEHLNWVLEMGNLPGAFK
                     RLMRFAVRRLRGRLTGTTQSDLSAEVAELLGDAEFTSSSMPLLGMGRDIPDGSMTLRN
                     DYLEIDWALGRSKDYFDGIKKTMRDIAQALQATFVVNPTFYFNRVATVHALGGCPMGR
                     DEHEGVVDPYGEVFNYPGLYIADGSVMPGPVGPNPSLTIAALADRFADRIIYR"
     gene            complement(16474..17952)
                     /locus_tag="AC162G_06950"
     CDS             complement(16474..17952)
                     /locus_tag="AC162G_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024748.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR5075584.1"
                     /translation="MISRRRRLFTARRVRLLGVLLVVVYVVAQFAAFRASLDRVPASW
                     TVAGTPFAGERIDEVVALMQAAFDEPVILHYMDEVRALPPDAVGFRFDAEATVEAFRR
                     KREESATAGNFLRHLIFQQPEPHDLPIVAEYSEELIRLKLSEMALLFDDPAQPPAPDL
                     QSMTLKPGQPGYMLDIVASVAPVAEGLKSLHERDAQLVVESQPAPPATIDQLRELIQE
                     RLKAFNGNAGVFLKALTSGDEIEIGGDVAYSGMSLMKIPIMAEVYRTLAQAPDVETHK
                     LLTETLGSESGNFTANLLLRQIGDGDSFAGVDRLTSSMRFLGLTNSFMALPYDQQDVT
                     PPPVATEANSQADVWTNPDPAMQTTPSDIGLLLEMIYQCGEGGGALVVAYPGAFTPEE
                     CRQMLDIMKENVITDAEGIPTLLRGGLPDGTPLAHKHGWDNDTRADASVAFTPGGDFV
                     LVVFVNTPGAWVDWDVANGIMVDIARAAYNYFNPGEVSAETP"
     gene            complement(17967..19280)
                     /locus_tag="AC162G_06955"
     CDS             complement(17967..19280)
                     /locus_tag="AC162G_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5075585.1"
                     /translation="MTMLPTAHSIARGFALTLAAALALTACRAEPVPTPVPTVEPTPV
                     VVQADAGAAGRIAVIDENGQVYTADPDGGGRVDLNASGTIPDAAIVWSRDGSRLAFSL
                     ASGNRGELVTIDASGQNRTTVYRDASSAAPFYLYWSPDNEHVGFLAAGAEGRLALSVA
                     QAGQDGSARLVARGEPNYFSWSSGGERMAVHIGGLRGYVGTHTLGETDTHNRDARPAL
                     FQAPAWSPVDDTYLFARAGSGRQDDLVIVRDDEEISLAEFDGGISFAWSPNGVRVAYS
                     TFDTTGEHYDRITLVRPDGSESRTLVDESHFAFFWSPDGTKIAYLTGRLGAPSTIGST
                     RRSPGLAVQARQELELTWNVVDVESGQSSTLASFVPTESFLFIVPFFDQYAQSISLWS
                     PDSRYLLLAGTPLARPQGIYRLDAEGSGDTLTYIGPGAFAIWSWR"
     gene            19540..20361
                     /gene="modA"
                     /locus_tag="AC162G_06960"
     CDS             19540..20361
                     /gene="modA"
                     /locus_tag="AC162G_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5075586.1"
                     /translation="MTVAKLLIGIVAAPAIVAACSAPQPAPSGSAPVTLTVFAAASLA
                     QAFAELGERFEVQHPGVEVVFNFAGSQQLAQQLAQGAPGDVFASADAAQMGVAIERGR
                     VRPGAPRDFARNRLVVVYPKDNPAGLVDLRDLARPELRGRLVLAAAAVPAGAYTLAFL
                     DKASADPRFGAQFKDAVLSNTGSYEENVRAALSKVVLGEAFAGIVYESDVAGEAANEV
                     GRIEIPDALNVLATYPIAPIADSARPDLAQAFVDFVLSPEGQDMLAKHGFLGASN"
     gene            20439..21254
                     /locus_tag="AC162G_06965"
     CDS             20439..21254
                     /locus_tag="AC162G_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5075587.1"
                     /translation="MSVPAGPPSPIRPIRRPPGLWRLLSLPLLAFFMLPIVALLLRAE
                     PEQLLASLRDEQVLQAIGLSLRTTLITVALTIILGTPVAQLLSRPHIRFHRAIDTLVD
                     LPTVLPPSVAGIALLLTFGRLGLLGAALEAAGIRIAFTPLAVVMAQMFVAAPFYIKSA
                     AIGFAGVDAELKSAAMIDGANAWQVFRSVVVPLSWTALISGSVLTWARALGEFGATIL
                     FAGSLVGQTQTMPLAIYLGFHIDLDMALTLSVILIGLSFVSLIVVKALLPGES"
     gene            21429..21671
                     /locus_tag="AC162G_06970"
     CDS             21429..21671
                     /locus_tag="AC162G_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075588.1"
                     /translation="MTSQTYEQIIVEGIKGLPEEMLAEIADFVYFVRKRATQPQAYEE
                     ELLSALMNAELRQMSRDEEEHLDQEFRDYDRRYPCD"
     gene            21646..22053
                     /locus_tag="AC162G_06975"
     CDS             21646..22053
                     /locus_tag="AC162G_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR5075589.1"
                     /translation="MTGATPATEFVTDTVGLVLRLEKRKMGAGAKSVFDSAESGGAII
                     YGPSLVLAEMLYLSEKGRITATLHDAADYMSQHSHCREYPLNLAVVQAAALIPDVREL
                     HDRLIAGVARLLGLQLITNDLSIQASSFVRTLW"
     gene            22105..22179
                     /locus_tag="AC162G_06980"
     tRNA            22105..22179
                     /locus_tag="AC162G_06980"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22140..22142,aa:Pro,seq:ggg)
     gene            22959..24821
                     /locus_tag="AC162G_06985"
     CDS             22959..24821
                     /locus_tag="AC162G_06985"
                     /inference="COORDINATES: protein motif:HMM:NF016906.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR5075590.1"
                     /translation="MASGGSDTANDCTVSASPCATIQHAVDQADSGAEIRVATGNYAG
                     VSVRDGVTQTVYLSKTVTIRGGYTTANWSIPDLEANPVLLNAQGQGRVLFITGDISPT
                     IEGLRITGGDTMAFSAYTGYGGGIYIITATATISANRVFGNNAPTGGGLYAAASQPRV
                     EHNLITSNTARYGGGVYLYNSPATLDANRISANTAITSGGGALFLFSSPVLTDNLFIS
                     NSAVTGGGLLLASSDALMLNTVIADNYASASGSALYLDSIFGRDCLPLLIHTTIARNT
                     GGDGSGIYATDDGMFGLSYAVMTNTIIVEHATGIVAAPGNGADLNGVLWFNNGANTGG
                     AGIIAVTSDITGTPAFDTDGYHLTAGSAALNRGVDTGMSRDIDGESRPMGAAPDLGAD
                     ELPIGLLVTKQVTPSVVPAGSPVTYTVRVTNTGAVSLTATISDLLPSQVVPTGILTWT
                     PTITAPGGVWVQQFSVTVAVGYSGTLTNVVQVSSQEGASGVFTATSSATVMPSFIMSK
                     WADPDPVEAGLPLTYTLVITNAGDAELHATVTDTLPGQVTPSGVITWTPLITAPGGVW
                     IQQLVVTVVEGYSGTLTNTLHATTVEGVTGVYTLTSRAIAPYLVYLPVVLRASP"
     gene            complement(24874..>25363)
                     /locus_tag="AC162G_06990"
     CDS             complement(24874..>25363)
                     /locus_tag="AC162G_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR5075591.1"
                     /translation="AMVLTGALTMDEAYRSIEWKSIFLIAAMLPVGIAMTKTGVASVL
                     GERLVALLGGLGPLAVAGGLLAIATLLTQVTSGPAVAVLLAPIAIHAAETLQVNPRGL
                     ALAVAYGCSLAFMTPLGHPVNVLVMGPGGYKFNDFLRIGTLLTLILIAVIILLLSLVQ
                     NG"
BASE COUNT         4715 a         7870 c         7957 g         4821 t
ORIGIN      
        1 ccctcgggga tgatcagatc cgcgtagcgc ttgctcggct cgacgaactc caggtgcatc
       61 gggcgcacgg tgtcgagata ctggtgaatg accgagtcca tcgaccgccc gcgctcggcc
      121 atgtcgcgct gcaggcgccg gatgaagcgc agatcggcgt cggtgtcgac gaagattttg
      181 acgtcgatca gatcgcgcag gggcttctcg acgaagatga ggatgccttc cagcagaatc
      241 accggcttcg gctcgacgcg gtgcgtctcg ctcgtgcgcg aatggttgcg gaagtcgtaa
      301 accggcatgt cgatcggctg gcccgcgcgc agtcgcttca agtgctcgat gagcagctcg
      361 gtctccagcg aatccgggtg atcgaagttg cggtgctccc gcgccgccgg cggcaggtcg
      421 tcccagtcgc ggtagtacga gtcctgctga atgaacgtca gtcgatccgc gccgatgcgc
      481 cccacgatgg cgcgcaccac cgtcgtcttg cccgagccgg ttccgccgga cactccgatc
      541 acaattcgag gagctggcat gtcagtttgg ctcacatcgc acctcgcatg gctagagggt
      601 ggtgggtcaa aggctcaaag cactcaatcc gatgcacagc gccgccgcga acatcagtgc
      661 ggcgcgccag cggctgtgct ccacgagcag gctcccgctc gacgtgatcc aggccgccat
      721 cagccaggcg tcgcgcggcg cgctgcccag ccacgcgccg cgctcgagca ggctctccgc
      781 cgcgctcgcc gccagcgcga tcgagatgcc aagccacagc gcgatgcgcg tccgatcgac
      841 gcttccccgc cacgagcgcg cagcggcatc gaggccgggc agggtctcga cgccggctgc
      901 gaaatttgcg agcaccgcaa tcgagatcgg cactgccgcc tctccgggca ggcgtgcagc
      961 atagatcagc agggcgctgg tggcgagcag catccaggga ggcgctttct cgaggatggc
     1021 cagaatcaaa ccgccggcgg cgatgaagac cgaggcgtac accgccagcg gcagcgcgcc
     1081 ggcagcaggc tgccatgcgg ggctgagcgc ggtgagcagg ccggccagca ggctgacgag
     1141 ggccgctatc cgtccagcct tggaccagtt gcggacgcgc ctgatccccg ccagagcggc
     1201 tgcgatgagt gtcccggcgg cgatgcccac aatcgcctcg agcatcatct cacgcttctc
     1261 ccggctgcca gcgtactcaa gtcaaattcg gcgtcgtcgg agacgaggcg agcgcctccc
     1321 tctccaggca cggtccagat gcgacgcgcg ggatacagag ccgcgaatcc cagcccggcg
     1381 acggcgacga cgaatccgat agcgacgacg aactgaaacg gggcgcgcac gacgtcgatg
     1441 gcggcgtgtg actcattcgc gaacgccagc cggtagccgt tcgcggccat agattggccg
     1501 gggacgatgg tctcgtcggc gatcgattct gcgctcgagc tgctcagaat ggacacgcga
     1561 taccggggag ccggatcgct ttccaacagc gaaacgccca cggcgagctt cgccgccggc
     1621 gcggcgaaga acttatccgg ctggttcgca tcgaatagga gcagtacctc gctcgacgcc
     1681 tcgccctgca cgctggtctg gagctggagc gcgcggccct ccccgtcctc acccgtcacg
     1741 cgcagtgcag gaagcacgtc tcgaacgtag atgctggcgg cgctttggat cgcgggcttg
     1801 cccggcgcgg cgatgccctg cgcgacttcg ctctcgccct ccagaaggcg gactgccacc
     1861 tcccccccag ggtcgatttc ggccgcgaga aacgtcaggg ccgtgtcctc gatccgggcc
     1921 gggacaccgg gcgtgacgct caaaccgtcg atgcgccagt cgccgagcga tgccgtcgcc
     1981 atgccggtca acatgatcag agatccggcg tatgcggcca tcgccgcgat gtatgccaac
     2041 gggaaccggt gagccagcag gtaggattcc gtcgagcaca cgcggtagtg gcgcatacgg
     2101 cgtgtgatct cttcagccgg ctctccggag tcgaatgtcc gggccgggga gtctggcggc
     2161 gggacggggc gagaagcggc gcgcattgcg gcgatgcgat ccgccaggcg ggcgatgagc
     2221 gcaaatccga ggagcgcagc cagcccgcga aagaatatcg agtggacgag gtcgaacagg
     2281 cccagcgtcg ccagcgcatc cgccgcagcg ccgaatcgaa ggtgcgcttc cgacagccag
     2341 cgcgcgtacg ctgccggatc gccgcgaggc gcttgcgcaa tcagcgccgc tgagccgagc
     2401 agaaacgcca gccccagcag ggcagcagcg aagaacacgt cccccgtggc gatgcgccac
     2461 gcggactgcc aggcgttacg ccggacgatg acgttggccg ggaggtcggt catgtgcgcg
     2521 cacgaaaaag ccccggacac ggggctgctc gcaagccacc catcggattt gaaccgataa
     2581 ccgaccgctt acgaagcggt tgctctgccg attgagctag ggtggcgacg cacgaaatta
     2641 taccagtcgc atcgaggcct ggcaagcggt gcacgataaa cggctcgtag tggcgacttt
     2701 agtttatgta acgactgacc ccactccact ttccttcggc tacgctcagg acaggcgcta
     2761 cgggggcaag cgagtcgtat ctaccatctg ttgagatagt ttctaagcgc ggtacgacct
     2821 cggaatgacg agcgtgacgg tcgtgccgac gccgacctgg ctcactacgt cgatctggcc
     2881 gccgtgcact tcgacgatct ccttcgtgat ggacagaccc aggccggtac cggtcgccca
     2941 cccttcgctg tcggcgacgc ggtaaaactt gtcgaagatg tgcggcaggt cttgctcggg
     3001 aatgccgcgc cccgtgtctt cgatcgcgac ctgcactgag tcgttccgct gcccggcgcg
     3061 cgcgttgacc ctgccgccgg gcgcggtgta cttgatggcg ttgctcagca ggttcatcag
     3121 gacttgcttg agccggcccg cgtcgccctc gacaggcggg aggccggccg gcgcgtcgac
     3181 ttcgacctgg atgttcttct tgtcggcctg cggcttcatc accgacacgc aatctcgcag
     3241 caggccggct atgtccacgg gcacgtgcgc gagccgcgcg cggcccgact gcaggcgcgc
     3301 caggtcgagg aagtcattga tcatcgccgt caagcgcgtc gattcctgca ggatggtttc
     3361 cacgaacgag cgctgctgct cgcgcttcag gtcgtcccgc atcagcagct cgccgtaggc
     3421 gacgatgctg gtgagcggcg tgcgcagctc gtgcaccatc tcggagatca agtcgctttg
     3481 ctggaataag cgggcgttct cgatcgcgac cgccgcgtgg gccgccagcg cggacagcac
     3541 atcgatgtcc tcgtcgtcga agggtgtgtt gtcgacgcgg ttgagcgatt ccagcacccc
     3601 gatggtgcgg cctttcacct gaagcggcag tgcgatcagc gcccgcgtct tgaagcccga
     3661 ctgctgatcg gggagcggcg accagcgcgg atcgctgctc acgtcatcga tgatctgcgg
     3721 tttgccttcg cgggctaccc agccggccag actgttcggc ggaacgacga cgcgcttgac
     3781 ttcctcgctc ttagttccgg tcgccgcctc gaagtacagg tctcccgttt tcgggtcggt
     3841 caacataatc gaggacgcct cggttcgagt gatttcacgc gcggcttcga tgatgagcgt
     3901 gagcagcgtg accaggtcca gcgtcgagtt gagaatgcgg ctcagctcca gcacgcggcg
     3961 gaagcgcgcc aggcgggttt ccagctcggt ggtgtgtggc ataggctctc gtcagccggg
     4021 tctcagctcc ggcgggcgtc gatcgcctcg acgatgcgcg gcagcacgtc ggcgacgtcg
     4081 cctcggatca atacgtcggc ccggtcatcc aggtaagttt cgccgaagtt gatgatcatc
     4141 aatctggctt gatgttccag cgccaggtgt ggcaagtcag ccgccggggc gacctccagc
     4201 gacgagccgg cgacgagcat aacgtcgcac tggcgcgccg cggccatggc gcgcagcagg
     4261 gcctgcgccg gcagcgcttc gccgaacagg gtcgcgttcg gcttcagcac gccgccgcac
     4321 gccgggcaga gaggcgtctc cccatcggtg atgaagcgcg ccaggtacga ctcagatggc
     4381 gtgacacggt aacacgcaag gcacacggct tcgcgtacgt gaccgtgcac ttcggcgacg
     4441 tgcatcgaac cggcctttgg gtgtagcccg tcgatattct ggctgatcac ggtgcgaatg
     4501 atgccggcgt gctcgagctg cgcgagcgcg aaatgcgcgg cgttcggctg ggcgtcgcgc
     4561 atgagcgtgg ccagcgggcg cagccagcgg taaaagcggt cgggattctc gcggaaagcg
     4621 taaatggagg cgacctccat ggggtcgctc gtctcccata gaccgacgtc cgcgctgcga
     4681 aagtcaggta tgcccgacgg cgtgctcacg cccgcgccgg tgagggcaac ggcatgccgg
     4741 gccgcacgca gcatgcgcgc cgcgcgctcg acaagcggtt gcatcacaat gtctgcatcg
     4801 tgcgccacga tcgtcgccgt cgggtgatct cagatcggtc ccagcatcaa gatgcgcgcc
     4861 agctcgcgga actgatcgga tacgatgttg ccgggctgaa ccatgataag aggcgcgccc
     4921 tgttccgatg cctggaaggc ggcctcggga accggggtga ccacaactgc caggtcacga
     4981 ttcaggatgc cctcgatcac ggccttgctc agcgttgccg ccgagggcga gcggtgaacc
     5041 acaacgggca tgaccttgtc tgcgccgatg ccggcttttt cgagctgcgc gagcatcgat
     5101 tgtgccagga gaacggcggg gcgctgaggc tccagcacga cgatcaggcg cgagcacagt
     5161 ctcaccgcgg ctcgcgcgcc gtcgtccagg cgagagccaa ggtcgagcac gacgacttcc
     5221 gcgaggcttg ccagttggcg gatgatagcg tccgcgtgtc gggccggaag gtccgttgtc
     5281 tcggcgcgct gttcgatagg cgacaggagc atccgcatgc cggagacgtg cccggtcagc
     5341 accgactcga cgagcttggc gctgacatcg tccgcctggc gcgacacgag cgcctggagg
     5401 ccgcttgccc gggtgatgcc gagttgcagc cccacgtcgc cggcggccga atgcaggtcg
     5461 ctgaggataa cgtgttggga cgcatcgtcc ttgccgccct gagcgagcgc gacggagacg
     5521 ttgatcgcca gcgtcgtcgt cccgacgccg cctttcgcgc cgatgaatcc caagatgcgg
     5581 gcgtgcgggc ggacgctcat ctcggcccgc gtgcgcgctc tgcgcagcag caccgcttct
     5641 acgcgcgacg acagctcggc gggatgaatc ggcttggtca ggtagtcgtc cgcgccggcc
     5701 tggaacccgg cgaccttgtc tccgaccagc gtcttggcgg tgaacatgat gatagggatg
     5761 tccgccgtct tgttattggc gcgcatgcgc cgggtgacct cgtagccgtc catgtccggc
     5821 atcatgatgt ccagaataat caggtcgggc tgctcggatt cggccttgct gagagcctgc
     5881 gcgcctccct gagcggcggc gatctgatac ccccggcgtt gaagcatcaa gccgatgagc
     5941 ttcaagcttt ccagatcgtc gtcaacgatc aagattttct cggccacgac gttttcacca
     6001 cgagctggga tatgtctgcg tgcagcctcg catgatgcaa cctgtcgaaa ccccataaag
     6061 cccaggctcg actcgcgtat cctgtgcacg ccattcggat tgtatagtac caccgcaggt
     6121 ttggcaagtc aaaccatcgg tagtaccgcc tcggcgtgcg ggccgccctg aaccggacac
     6181 ccggcagctg gataactcct gtataataag cacatgccca atgcgggtct gtccaattgg
     6241 tcgcccctct tgatcctcgc cgcggctgcc gcgctcgccg cgctggcctt tctggtgcga
     6301 cgccgcgtgc tgtcgcgccg cgagcttgag cgccgctcgt ccgagctgga tgcgctggtc
     6361 gacttcggtc gctcgatcgc gcagtcacgc ctcgacgtgg acgcgctgtg cgagctgatc
     6421 caccgcaagg cttcgcagat cgtggatacc agctcgtttc agatcgggct gttcgaggac
     6481 gaccagtatg ccattcgcct gtggattcgg gagggcgagc gtctcccgcc cgcgaagttc
     6541 gacctggcgc agaacggcgg cctgatcggc tggctgaggc agtccaagca gcccctcctc
     6601 attcacgact tcgagcgcga gatcgacgcg ctgccggcgc gcccgcgtta tctgagcaac
     6661 cagccgccgc gctcggcggt gttcgtgccg atcatcgccg gcgaggaggc ggtgggcgcg
     6721 ctctccatcc agaacgaccg ccccaacgtg ttcgacgacg atcacgttcg cctgctgtcc
     6781 atcgtcgcca accaggctgc gccggtcatc gccaacgccc ggctgctgga agccgagcgc
     6841 cggcgggcga cgcagttggc gctcatcggt gaggtcagcc gccaggtcgc tgcgatcctc
     6901 gagatcgatg tgttgttcga ccgggtggtc gaattgatcc agtcgcactt caattactac
     6961 ttcgtcgccg tcgccctgcg cgaggaagac agcgacgagc tggtgctcgc cggcacgacg
     7021 gcgaaagacg cgcgcgggcg gcgcctgaag cccgggcagg ggatcatcgg ggcggtcgtg
     7081 caatccgggg agccgctgct cgtgaacgac gttctcgcgg attcgcacta cgtcgcgctg
     7141 gcctttcttc ccgagacgcg atcggagctg gccgtgccgc tcaccttcgg ccagcgcatt
     7201 ctcggcgcgc tcgacgtcga aagcagcgag ctgaacgcat tcacccagga agacctcttc
     7261 atcctgcgca cgctcgccga tcaggtggcg atcgccacgc acgaggcgcg cctatacgcc
     7321 gccgagcgcg agcaggcatg ggtcagcacc gcgctgctgc aggtggccga ggcgacgacg
     7381 caggcgacga gcgtcgagga agtcctcgag accgtcgtgc gcatcacacc catgctgtcc
     7441 ggcgtggatc gctgcgggat cctgctgtgg gatgcaaccg ggtcagtatt tcgcgcggct
     7501 gcctccttcg gcctggacga gcgggcgcca gattttgatg cgctcacgct cggcgccgac
     7561 gactggcccg acctattcgt ggctgaggcg acgggagaag cccttcagtt ggactacgcg
     7621 cccggccgcc tgagagaggc gttcgggccg gggccactgc tggcgctgcc gctgtatgcg
     7681 cgtggcgagc gcgccggcgt gatgctcgtc ggctcgccgg agggcagcca gcttgcgaag
     7741 cgcaaggcgg cgctgatcgc cggcatcgcc aaccaggcgg cgctcgccat cgagtcgtcg
     7801 cagttgctcg cttcacagcg cgaagaggcg tgggtgaaca tggcgctgct gcaagtcgcc
     7861 gaggcggtcg gttcgcagac ggagctgcgc gacattttga cgaccgtggt gcgcctgacg
     7921 ccgctgctgg tcggcgtgga aacgtgcctg attctcctgt acgacccggc gagcgcttcg
     7981 ttcgtcgcgg gtgacgtgta cggcattccc agggagcgcc tcaagctgtt cgccgatctg
     8041 cgcgtgccac aagcagcctg gtctctcggc gaagcgccgg cggatatacc gcttgaggtc
     8101 gtcgaacgac tgcagctcag cgcgccgctg gcctggccgc tagtcgccaa aggcgacgtc
     8161 gtcggcgtga tggtcatcgg cggcggaacg gaagcgcgcg ctcccagcgc gcgccacacc
     8221 aacatcctgg ccggcatcgc cagccagacg gccatggcgg tgatgaacgc gcagctcgcc
     8281 gacgaggccg tcgcccgcca gcgcctcgag caggagctgc gcgtcgcgcg caagattcaa
     8341 gagagttttt tgccggagcg gtgcccctcg ctgcccggat ggcagatcgc cgcgttctgg
     8401 cgctcggcgc gccaggtcgg cggcgacttc tacgatttca tcccgctgcg cgatgggacc
     8461 gatcgcctgg gcatcgcaat cgccgacacg gccgacaagg gcgtgccggc ggcactgttt
     8521 atggccttgt cgcgcactct gctgcgcgcg gcggcgatcg gcgggcgcgg cgcggcggag
     8581 gctctcacgc gcacgaacga cctgatcctc gccgatgcgc gctctgacct gttcgtcacg
     8641 atcttctata tcattgtcga tgcgtcttcg gagatggttc gcttcgcgaa cgccggccac
     8701 aatccgaccc tgctcgtgcg cgcggtaagc ccgattcccg aatatctcac ggagcacggt
     8761 atggcgctgg gcgtgatgcc cgacgtcaca ttcgaagagc agagcctggc tgtagggcag
     8821 ggggatgtcg tcgtgctgta caccgacggc gtgaccgagg cgcggaatga agccggcgaa
     8881 gaattcggcc tggaacggct ggagcgctgc gtaatcgaca acatgagcgg ctcggtcgac
     8941 gacatcgtgc aggcaatcaa ggatgcgctg cgcgcgcacg tcggcgacga gccgccgttt
     9001 gatgatatta cgtttgtggt gttaaagaga gtcaacgaat agggagcaaa tcagcgaatc
     9061 aacgaatgaa gacgacgtcg ttgccggcgc agggtcatcc gggcacgtca ggcccgattc
     9121 gccgcgtggc ttggcgagcc tcaatgcacc cgaatgaact tggcagtgga gggcacgcca
     9181 tcggagttga cgatgaacag catataatct ccgggcggcg caatgctcgg atcgggtgga
     9241 gcggcgacgg tgagctgcca tggggatttc tcatcgatct tcagcccgac gtagcgctga
     9301 tccatgttgt tgttgtgcgt cgtcgtggtc ggacggatga gggccacgga tgtgatggac
     9361 tgcccatggc gggtctcgac atcgaacttt tctccgtacg tgacttgcga gggagcccag
     9421 tcgatgaccg gccgtgggcc gcgcgcgaca tagggcgggc tgaatacttc gaggcgtaac
     9481 tcgtttctat ccggcggcca ctcgtgtccc gtgctgcccg cggtgagcac acgcccatcg
     9541 ggcagcagga ctgcggtcga gtggtaaagt cgtgcaacct ctgcttgggc cagtctctgc
     9601 caggtctccg ttaccgggtc aaaacgctcg gcttcccgga cctcgccgtg gttatgatcg
     9661 gcaacgcctc gctcggcgcc gttgatgacc aaaacagacc catcgggcag cagtggcgca
     9721 tccaccagaa tgcgtggctt cgcgagaggc gccgtttctc gccaacggct ctcgtgcgga
     9781 tgttgaatat ccagttcgaa gatctcaact cggtcaattg ccggggtgtc ctcacccggt
     9841 tcgagtgcgg tgctgccgcc cacgatcaag acccgcacgt agggatcgtc gggccgtatc
     9901 ggcagcagca cgctgctgcc ctggccgggg tacgtccgcg tgcctgggct gttcatcgta
     9961 aacacttggg gaagccattc ctgtctgttg agatcgaaca atcgagtctt gttcccggag
    10021 tgaacgaaca ggaaccctcc gggcaagacg tgcatgaacg gatacaggcc gatatcttgc
    10081 aggaaaggct gtggagccga caacgtactg gtctccggat caaagatctc gcatgtagca
    10141 ttcatcgcga accgatcgct tcctgtcaat cgttcggcca tgggcgcaat acaatgatgc
    10201 cagtatccgg aaacgatcag ggccttgccg ttgggcagcg tgacgcacgt aggataccat
    10261 cgcgctcgtg gcatatccgc gtgtcgcgac cacgtttccg tgaccggatc gaacgtatga
    10321 acatcgtggt cagcgcctcg accaaagcca agcagcgaga taatgacttg ccagggaacc
    10381 tgtgcgcacg actgtccacc cgctgcgagc aggcgtccat ccgggaggat gcagtggccg
    10441 ctgcagaaga ggttgcgcga cagatcgggg tacgtgacat cgccgtttac aggatcgtac
    10501 agcgcagact gccctctgtt gatggctgca tagtcatgca tatcggccga gaataaaagc
    10561 accttcccag tgttcaacag cgccatgtgg acaccgacaa cgttcaggct ggccgcgatt
    10621 tgccattcac ctggtacggc ttctgtttca cttatcacga tttccgtgta gagctgcacg
    10681 gcacacctcc tgcgcaaaga tagaaaacgc aacatctatt tccgttgggc caaaccatcc
    10741 gatcgccaca cccggccgga acgaatacag gcgatcactg cactcggaca aacttcgcga
    10801 tggaaggcac cccgtctgtg ttgacgatga agagcatgta gtagccaggt ggcgccacac
    10861 tcccatcggg cggagccatc accgtgagtt gagccgcgtt tctgcactcg atctgcagac
    10921 cgacgtagcg ctgatccatg ttgttgttgt gcgtcgtcgt gctcggtcgc acgaaggcga
    10981 ccgattggat ggaccgcgca tcgccggtct cgatattgaa actcgcccca tacgacacct
    11041 gcggcggagc ccagtcgacg acgggccggg ggccgttcgc gagatacggc gggctgaaaa
    11101 cttcgaggcg cagctcctgt tcatccggcg gccattcgtg tcccgtgctg cccgcggcga
    11161 cgacacgccc gtcgggcagc agggctgcgg tcgagtggta cagtcgttgg atgcttgccc
    11221 gggcgagtgt ttgccaggtc tcggttgcgg gatcgaacag ttcgggttcc agcacggcgc
    11281 cgtggttatg gtcgccggtg ccttgtttcg ctccgccgat gaccagaatg gtgccgtcgg
    11341 gcagcagcag cgtttctgac acaaaacggc gatgagtcaa cggtgcggtt tctctccagc
    11401 ggctctgtcg aggatcttca gcattcagtt cgaagatctc aactctgtcg gttgccggag
    11461 tttcctcagt cggttttaat gcggtgctgc cgcccacgat gagcactcgg gccggctgcg
    11521 cgttgcccgg cgagatcggc aggagcacgc tagttcccgg agcggggtat atccgcgtgc
    11581 ccgggctgtt catcgtaaac acttggggaa tccatgcctg tttgttcaga tcgaacaatc
    11641 gagtcttatt ccaggaatga acgtaaagga atccaccagg caagacgtgt acgaacagat
    11701 acacaccgat gtctttgagg aatggctgcg gagccgtcaa cgtgctggtc tcgggatcga
    11761 atatttcgaa ggtatcgttg attccgaacc gatcgcttcc tgtcagtcgc tcgacaatct
    11821 gagcgacgat gcgattccag ttgccacaga cgagcatcgc tttgccatcg ggcagcgtca
    11881 cacaggtggg ataccagcgc gcgcgcggca agtctgtgtg ccgggaccat ttttccgtca
    11941 ccggatcgaa cgtatggatg tcgtggtcaa cgccacgacc acggccggcc agcgcacgaa
    12001 ggaattgcct caggttctgc ccgtacgcct gcccgccgac caccagcacc cgcccatcgg
    12061 ggagcataca gtggccgctg cagagaagat ggcgcgacaa atccgaattg gtgacgctat
    12121 cggttgccgg atcccacagc gcagccttac ccttatccat cgcttcgtag tcgtgatgat
    12181 cggctgagaa taagagcacc tttcccgtgt tcaacaacgc catatggacg ccgaccacac
    12241 ccaagttggc gaccgcgaca tcccatttgc catgcacgct ggcatctatc ttgctcttca
    12301 cgatctccag gcgatcaggc atgacactcc ttctgcgcac agaacaaacc ctgcgttttg
    12361 cgacaataaa cgacaaaacg cagggttttt gggcatggtt tactgaaccc catgaggaca
    12421 ccaagaatac ccttacaaat tcatacgatt ctttgacaat cccgctccca gatgtggtga
    12481 attctggcgc aaattcggca gccccaccag aagactgatc ggtcccagct ccagccagcg
    12541 cagggcgttc cggttctgca gcgcgcgcag cgccgacctc acgcctgcgc ggacgaagtt
    12601 aagctctcaa actgtaacgg atgttcagcc atgcgttaag gtagcaaccc gggttataat
    12661 atagcaacag actaacatgg aaaaatttat agaaaacgta gcctaaaggg tgcattcata
    12721 ttcggaggga aaaatgaaat tcgagacacg tcggacctgg aagaacattg cgaagaatca
    12781 ggttgccgag ccgctacgga tatataaacc cgacagcctg gagcagatca ttgagattat
    12841 cagagaggcc gagaggcgac gcgttcgagt tcgagccatc ggctctggcc attcgtggtc
    12901 tgatgtcggt ctcacgacag gtttcctggt aaagccggat agattgaata gagtcgtccc
    12961 gcttgacacc tcgttgctga atggagatgt cgatacgtct acactattcc acatagaatc
    13021 gggcatgcgt atccgcgaac tcaacgaaga gctagaccgc agagggttgg ccttgtccaa
    13081 tatggggggg tatgatggac aaacctatgt gggagcgatg tcaacatcta cacatggctc
    13141 ggggctggaa ttcggaccct tgcccgattt cgtcgagacg gtcgagatcg tctcggatgg
    13201 cggcgtcgtc tacaggattg aacccaccaa tgggatcact aaccgctcgg cttacgaaaa
    13261 gaaataccct gacaagaaag tcggaaaact catccaagac gacgactggt ttaatgccgt
    13321 tgtcgtcggc atggggtgta tgggcatcat ttattctgtc attctccgcg tcgcggagaa
    13381 gtactggctg aaagaagtgc gcaccctgag cacctggggt aaggttaaag aggatctcat
    13441 aaaagggcgt gtactcgcag acaaccggca ctacgaagtc ttagtcaatc cctacaaggt
    13501 gggaggagcg caccgatgtc tcatcacaac gcgcaacccc acagatcctc ctcgcaataa
    13561 accacacgac aaattaaatc ggaattatct aatcgagctg ttggccaccc tttcgttcat
    13621 acgccgagtc ttgaatctcg ttttagatct cttaccggag atcacgcctg agttgatgaa
    13681 ttctgccatg gaaggccttg tggacgacga atacaccaat gtaagctata aggtattgaa
    13741 cataggaacg gcaaaccaag tcaccgccta ctccgcggag atcggatttc ctctcaaaga
    13801 gaacaagtat atagatgcag tggataagtt tctagacatg gccgaagaac taaaatccgt
    13861 tggcgtcgtc tttctcagct cgccgatttc cttgaggttt gtgaacgctt caagcgcctt
    13921 tctgtctccg caatacggat atgatacctg tatggtagag atcatcgcgc taaagggtac
    13981 gcaaggatac ttcgagatga tagaacagtt tgagaatgtt ttatacgggt tttccggtcg
    14041 tcctcattgg ggacaggtaa acagcctcac tgcgagccac aacctgatag actcgatgta
    14101 tccgcagtac cataaatgga tcgatgttca tcgtcaactc aacaaaaacg gcacgtttga
    14161 cagctctttt tccaaacgcg tgggtttcac tgagcatacc ttcttaccat cataatcagc
    14221 atctatcggt gcctactctc gtgtagtgca ccctgtgatt caccagagtc cggtttctat
    14281 atactcctct agcgatagat gattcgatcg gcgaaccggt cggcgagggc cgcgatggtc
    14341 agcgatgggt tcgggccgac cgggccgggc atcaccgagc catcggcgat gtacaggccg
    14401 ggatagttga agacctcgcc gtatggatcg accacgcctt cgtgctcgtc acgtcccatc
    14461 gggcagccgc ctaaggcgtg aacggtggcc acgcgattga agtagaaggt ggggttcacg
    14521 acgaatgtgg cctgcaaggc ctgggcgatg tctcgcatcg ttttcttgat gccgtcaaag
    14581 tagtctttgg atcgtccgag tgcccagtcg atttccaggt agtcgttgcg taaggtcatg
    14641 ctgccgtccg gaatgtcgcg tcccataccc agcagtggca tcgaactcga ggtgaactcg
    14701 gcatccccga gcaattcggc gacctctgca ctcagatcgc tctgcgtcgt tccggtgagc
    14761 cttccgcgca gccggcgtac cgcgaaacgc atcaggcgct tgaacgcgcc gggcaggttg
    14821 cccatctcca ggacccagtt caaatgctcc ggataccctg cgtcttcgat gtagtgtccg
    14881 cgcatctggc tgccgtccag cacatcgggt atgcgaatcg cgctggtgat cgccgggcca
    14941 tagcccgcat ccagaaagcg cggtaccagt ttgccgtcct tcacttgacg gctgtggatg
    15001 gcgaatgtga ggaaatcgcc gttgccgcaa aatcgagtcc ccaactgctt gctgagatga
    15061 ggaaaggcgt tccgattctt gagcagcagg aaggtcgtgc cgagtgtgcc ggcggcgagg
    15121 atgagtcgat cggcggtgag cgtgattgcg ggcaattggg acgtcgcgaa ttttcgccct
    15181 tcgcgcgctg ccggatcgtg ttcgacatag cggacgacgt agcccccgcc aacgcgcggc
    15241 tcaaacgacc gcacttcgca caacgtccgg atgtctgcgc cgaggcgcct ggcttcggac
    15301 aggtagttgt agtcgagggt gtttttgctg ccgtagttgc agccgatgtc acattcgccg
    15361 cacaggcggc aggtatagcg ggtgcgtctg tgtaagttcg gatgcgcttc cttaatcggc
    15421 tcgccgggca gcggcgtttc gccggggttg ctaaaggtga tcgccagatt cggcaactgc
    15481 cattccaggt tgagtttctc ggcggcagct ttgaattctc tggtctttgc cgtttgatcg
    15541 tagggcgcat gctcgaaggg atagcgctgc gcattcagca tcttctcaac ctgatcatag
    15601 tgagggtcga gatcggcgcg cgtcaccggc cagtattcat agccgccctt gtgaatgtct
    15661 tccttcacga accacttctc gtctttgcgg atcaaaacgt tggcataaat caacgatccc
    15721 ccacccaggc cactggacac cacggcttcc aaattcctga accgccagat gttgaacagg
    15781 ccgtacaggc cttcgctcgg atcccaaaaa ttccgcctca ttcggtaagg gctgcgcggg
    15841 aatgaattgg gggggtaagc cttgccgcgt tcgagtacgc atacccgcaa gccggcttcc
    15901 gcgagacggt acgccgtgac cgacccgccg aatccggaac cgacgatgac tgcatcgaag
    15961 tgtgtttcag ccatgcgtgc tctcctcact ttaccagcca tgaacgggcg gcgctcaatg
    16021 cgttcgtttc ccaactgcac gagcggtacg gcgacagttc gtgcgcgtgt gtgccgtcac
    16081 tatactctac aatgacttgt gtcgtcaagt catatctggg tgtactgggc aggaccgccc
    16141 cctccacgcc gaaaacaagc tcgtccagaa actcgacggc gaggatggtg agtgtgagtc
    16201 aacggggagg atggaagtgc tcgggcctca gcatggctgc atcataacag gtgttgtaac
    16261 tatgcgcgcc ggtcggacga gagattcctc actccgttcg gaatgacact gctcggcgat
    16321 cggcgccaag tgtgtcatgc cgaggaggca cgacgaggca tctcgaccgc gacgcggcgc
    16381 gcgcgccggt cggacgagag attcctcact ccgttcggaa tgacactgtt gaagtctgga
    16441 atttgaactt tggagtttgg agttagcaac tcctcacggc gtctccgcgc tcacctcgcc
    16501 ggggttgaag tagttgtacg cggcacgcgc gatgtcgacc ataatgccgt tggcgacatc
    16561 ccaatcgacc catgcccccg gcgtgttgac gaacacgacc aggacgaaat cgccgcccgg
    16621 cgtgaacgcg accgacgcgt cggcgcgcgt gtcgttgtcc cagccgtgct tgtgcgcgag
    16681 cggtgtcccg tccggcagcc ctccgcgcag cagcgtgggg atgccctccg cgtcggtgat
    16741 cacgttctcc ttcatgatgt cgagcatctg ccggcattcc tcgggcgtga atgcgccagg
    16801 atacgcgacc accagcgccc cgccgccctc accgcattgg tagatcatct ccagcagcag
    16861 gccgatgtcg ctgggcgtcg tctgcatcgc cggatcggga ttcgtccaca catctgcctg
    16921 cgagttcgct tcggtggcga ccggaggcgg tgtgacatcc tgctggtcgt acggcagcgc
    16981 catgaaggaa ttcgtcaggc ccaggaagcg catcgacgaa gtcagccgat ccacgccggc
    17041 gaaggaatcg ccgtcgccga tctggcgcag cagcaggttg gcggtgaagt tgccgctttc
    17101 cgagccgagc gtctcggtga gcagcttgtg tgtctcgaca tccggcgcct gcgccagcgt
    17161 acgatagacc tcggccatga tgggaatctt catcaggctc atgccggagt aagccacgtc
    17221 gccgccgatc tcgatctcgt cgccggacgt cagagctttc agaaagacgc cggcgttgcc
    17281 gttaaacgct ttcaggcgct cctggatcag ctcacgaagc tgatcgattg tcgcaggcgg
    17341 cgcgggctgc gattcgacga ccagttgcgc gtctcgttcg tgcagcgact tcagcccctc
    17401 ggccaccggc gccaccgacg ccacgatgtc gagcatgtaa cccggctggc ccggcttgag
    17461 cgtcatcgat tgaaggtcgg gcgcgggcgg ctgcgccggg tcgtcgaaga gcagcgccat
    17521 ctcagacagc ttcaggcgga tgagctcctc ggaatactcg gcgacgatcg gcaggtcgtg
    17581 cggctcgggc tgctggaaga tcaggtgtcg caggaaattg cccgccgtcg cgctctcttc
    17641 gcgcttgcgc cggaacgcct cgaccgtcgc ctcggcgtcg aagcggaagc cgacggcgtc
    17701 cggcgggagc gcgcgcactt cgtccatgta atgcaggatg accggctcgt cgaacgcggc
    17761 ttgcatgagc gcgacgacct cgtcgatccg ctcgccggcg aacggcgtcc cggctaccgt
    17821 ccacgaggcg gggacgcgat cgagcgacgc gcggaaggca gcgaactgcg cgacgacgta
    17881 cacgacgacg agcagcacgc ccagcagtcg cacgcggcgg gcggtgaaga gacggcgtct
    17941 tctagaaatc aatgcttcct cttatgctac cgccacgacc agatggcgaa cgcgcctggg
    18001 ccgatgtagg tcagtgtatc gccgctgcct tcggcgtcga ggcggtagat gccctgcggg
    18061 cgggccagcg gcgtgccggc cagcagcagg tagcggctgt cgggcgacca cagcgagatt
    18121 gattgagcgt actgatcgaa gaacggcacg atgaacagga agctctccgt cggcacgaac
    18181 gacgccagcg tcgacgactg tcccgattcg acgtcgacga cgttccacgt cagctcgagc
    18241 tcctgtcttg cctgcacggc cagccccgga ctgcgccggg tcgacccgat cgtgcttgga
    18301 gcgccgagcc gcccggtcag gtaggcgatc ttcgtcccgt ccggcgacca gaagaacgcg
    18361 aaatgcgact cgtcgacgag cgtgcgcgac tcgcttccgt ccggcctgac gagggtaatc
    18421 ctgtcgtaat gctcgccggt tgtgtcgaac gtgctgtagg cgacgcgcac gccgtttggc
    18481 gaccaagcga agctgatgcc gccgtcgaat tcagcgagtg agatttcctc gtcgtcgcgc
    18541 acgatgacga gatcgtcttg ccggcccgat ccggcccgcg cgaagagata cgtgtcgtca
    18601 accggggacc aggccggggc ctggaacagc gccggtctgg cgtcgcggtt gtgcgtgtcc
    18661 gtctcgccga gcgtgtgcgt gccgacgtat ccgcgcagcc cgccgatgtg cacagccatg
    18721 cgctcgccgc cggacgacca agagaagtaa ttcggctcgc cgcgcgcgac gagcctggcc
    18781 gatccatcct gcccggcctg tgcgacgctt agcgccaggc ggccctctgc gcctgcagcc
    18841 aggaagccga cgtgctcgtt atccggcgac caatacaggt agaacggcgc ggccgaggat
    18901 gcgtcgcgat agaccgtcgt tcgattctgg ccgctcgcgt cgatcgtcac cagctcgccc
    18961 cggttgcccg acgccaggct gaaagccagg cggctgccgt cgcgcgacca cacgattgcg
    19021 gcgtcgggaa tcgtcccgga cgcgttgaga tcgacgcggc cgccgccgtc cggatcagcc
    19081 gtgtacacct ggccgttctc gtcgatgacg gcgatgcgac cggcagcgcc ggcatctgcc
    19141 tgcacgacga ccggcgtcgg ctcaacggtt ggcacaggtg tgggcaccgg ttccgcccgg
    19201 cacgcggtga gtgcaagcgc ggctgccagc gtcaaggcaa agccgcgtgc gattgaatgc
    19261 gccgtgggta acatcgtcat aagctcacgg gctttccggg aactttgaaa attataccag
    19321 tctttcgctg ggagtcccgc ttggggtcag tggttttgca ccaggtgtca cgtgccatgt
    19381 gccaggcagt cccgctcgcg cccgccgggt gacgtagtgg caacttagtc gctgccctaa
    19441 cgactgaagt cgtgactact attcgctttt gttacggggg caagtgagtc gttaatgctg
    19501 tcaaaagagg tttgggcgct cgcagttata attgccgtta tgacagtcgc caaactcctg
    19561 atagggatcg tcgccgcgcc cgcgatcgtc gccgcttgca gcgcgccaca gccagccccg
    19621 tcggggagcg cgcccgtcac actgaccgtc ttcgccgcgg cttcgttggc gcaggctttc
    19681 gccgagttgg gcgagcgatt cgaggtgcag catccagggg tcgaggtcgt cttcaacttc
    19741 gccggctcgc agcagttggc ccagcagttg gcgcagggcg cgcccggcga cgtgtttgcc
    19801 agcgccgacg cggcgcagat gggcgtcgcg atcgagcgtg gacgcgtgcg gcccggcgca
    19861 cctcgcgatt tcgcgcgcaa ccgcctcgtc gtggtctatc cgaaggataa cccggccggc
    19921 ctggtggatt tgcgcgacct ggccagaccc gagctgcggg gtcgcctggt gcttgccgcc
    19981 gccgccgttc cggcaggcgc gtacacgctc gccttcctcg acaaggcgtc cgccgacccg
    20041 cgcttcggcg cgcagttcaa agacgccgtg ctgagcaaca ccggctcata cgaggagaac
    20101 gtgcgcgcgg cgctgagcaa agttgtcctg ggcgaagcct tcgcgggcat cgtgtacgag
    20161 agcgacgtag cgggcgaggc agcgaacgaa gtcggccgca tcgagattcc cgacgcgctg
    20221 aacgtgctcg cgacgtatcc gatcgcgccg atcgcggaca gcgctcgccc cgacctggcg
    20281 caggcgttcg tcgacttcgt gctgtcgccc gagggacagg acatgctggc gaagcatggc
    20341 ttcctcggcg cgagtaactg actgccgata cccgaactga tgcgtgagcc ttctccaacc
    20401 tttgaacttt gagctttgga gattggatta aggagcggat gtccgtgcca gcaggcccac
    20461 cttcaccgat acgcccgatc cgccggccgc ctggcctgtg gcggctgctt agcctgccgc
    20521 tgctcgcctt cttcatgctg ccgatcgtcg ccttgctcct gcgcgccgag cctgagcagt
    20581 tgctcgccag cctgcgcgac gagcaggttc tgcaagcaat cggcctgagc ctgcggacga
    20641 cgctgatcac cgtcgccctc accatcatac tcggcacgcc cgtcgcgcag ctcctgtcac
    20701 gcccgcacat ccgtttccac cgcgcgatcg atacgcttgt cgatctgccg accgtcctgc
    20761 cgccgtcggt cgcgggcatc gcgctgctgc tgacgttcgg gcggctggga ctgctcggcg
    20821 ccgcgctcga agcggcgggc atccgcatcg ccttcacgcc gctcgccgtc gtcatggcac
    20881 agatgttcgt cgccgcgccg ttttacatca agtctgccgc gatcggcttc gccggcgtcg
    20941 acgccgaact gaagtcggcg gcgatgatcg acggcgcgaa cgcctggcag gtgttccgct
    21001 cggtcgtcgt cccgctgtcg tggacggcgc tgatcagcgg cagcgtgctg acgtgggcgc
    21061 gcgcgctcgg cgagttcggc gcgacgatcc tcttcgccgg gagtctggtc gggcagacgc
    21121 agaccatgcc gctcgccatc tacctgggct tccatatcga cctcgacatg gcgctcacgc
    21181 tgtccgtcat cctgatcggg ctgtccttcg tgtcgctgat cgtcgtgaag gcgctgctgc
    21241 cgggggagag ttaagagact tcggaagtct tagggcttgt tctcgcaggc aatggtgtcc
    21301 ggcggtttcg gtagttccgc aggcagtatg acacctaaag acttccgaag tctgaagatc
    21361 gaacatagaa tctgtgttac actatgttcg tgttgagcga ttcggcgcac aatcagagag
    21421 gctgcacgat gacttcacaa acatacgagc aaatcattgt cgagggcatt aagggcttgc
    21481 cggaggaaat gctggccgag attgccgatt ttgtctattt cgtccgcaaa cgagcaaccc
    21541 agccgcaggc gtatgaggaa gagctgctat cggccttgat gaacgccgag ttgcggcaga
    21601 tgagccgcga tgaggaggag catctggacc aggagtttag ggattatgac cggcgctacc
    21661 cctgcgactg aattcgtcac ggatacggtc ggccttgtgc tgcgcctcga aaagcgcaag
    21721 atgggcgcag gggccaaatc cgttttcgac tcagccgagt caggcggcgc gatcatctac
    21781 ggtccctcac tcgtcttggc agaaatgctc tatctttctg aaaagggcag gattacggcc
    21841 acacttcacg atgcggctga ttacatgagc cagcattctc actgcaggga gtatcccctg
    21901 aaccttgccg tcgttcaggc cgccgcgctc atccccgatg tgcgcgagct tcacgatcgg
    21961 ctcatcgccg gagtcgcgcg attgctaggt ctgcaactga ttacgaacga cctgtctatt
    22021 caggcatcgt cgttcgtgag aacgctctgg taatatcgtg cttgccactt cgcgccgcgt
    22081 ctgttataat cgcgagagtt cgttcggggt gtagcgcagt ccggctagcg cactacaatg
    22141 gggttgtagg ggccgtgggt tcaaatccca ccaccccgac taaaagaccg tattatcagg
    22201 ccatcgtcag gcttatgata cggtcttttt cttgaagttc tatctagttg acgaggggca
    22261 acaccgttac tacaataatt ggcaaacccg tcagctctcc gaacatttcg cctctgattg
    22321 gaagcgctac gcatgggatc aggttctggc gcaatctggc gcctcgaccc gggtattcat
    22381 ttcgtttgac ccgcctacgg aggagagtgc tcccgtagat tactatttga ctctctcgta
    22441 cgagtcgttg ggcattgcaa tagactacaa gggcccagca caatataatg tcgaggcctc
    22501 attggtgcgt gcgtgcccca gcttcaacga agtttctggc attaacttgg cgttggttac
    22561 tgcagaacaa ggcgattcaa taactgattt cattttggac ttgtcggggg ccaacgcgct
    22621 agcagtgtgt cggagagggc gactgttcag tccgttccgg cagtggtaaa ctggtgcata
    22681 tgttgattca gcaagctgag gcgatgcgat aagcaggcga aagccctacg agagatgagc
    22741 accggtgcat gatgcggctg cgggggccaa accctgaaca cgtttgactc aatcggaagc
    22801 gcctctcgtc gatgaggagt tcagtgatga gacacttcat agtcagactc gcgaccgccc
    22861 tggcgttcgg gctggggctg tgcgcggcaa gcgtgtcagc gttgagcact tcaactgtgg
    22921 atgtgcctgc tgcggttgcc gccggtctag tcctatacgt ggcgtcaggc ggcagtgaca
    22981 ccgccaacga ctgcaccgtt tcggcaagtc cttgcgccac gatacagcat gccgtcgacc
    23041 aagctgactc cggagcagag attcgcgtag ccaccggcaa ctacgccggc gtgtcggtac
    23101 gcgacggtgt gactcagacc gtctatctca gcaagaccgt caccatccgg ggcggctata
    23161 cgacggccaa ctggagcatt ccggacctgg aggcaaaccc cgtcctgctc aatgcccagg
    23221 gtcagggccg cgtgctcttc atcaccggcg acatcagccc gacgatagaa gggctgcgca
    23281 ttaccggcgg cgatacgatg gccttcagcg cttatactgg atatggaggc ggcatctaca
    23341 tcatcactgc cacagcgacg atcagcgcga atcgcgtatt cggcaacaac gcgcccaccg
    23401 gcggcggtct gtacgctgcc gccagccaac cgcgcgtcga acacaacctc attacctcaa
    23461 acacggctcg ctacggaggc ggtgtgtatc tgtacaatag ccccgcgacc ttggacgcca
    23521 accggatctc tgccaatacg gcgattacca gtggtggagg cgctctcttc ttattcagtt
    23581 cccccgtcct caccgacaac ctcttcatct ccaacagtgc cgtcaccggc ggcggactcc
    23641 tgctggcctc cagcgacgcg ttgatgttga ataccgtcat cgccgataac tacgcgtcgg
    23701 catccggcag cgccttgtat ctcgacagca tcttcggccg tgattgcctg ccgctcctga
    23761 tccacaccac catcgctcgc aataccggcg gcgacggcag cggcatctat gccaccgacg
    23821 atggcatgtt cggcctgagc tatgcggtga tgaccaacac catcatcgtc gagcacgcga
    23881 ctggaatcgt ggcggctccc ggcaatggag ccgatctgaa cggcgtgctg tggttcaaca
    23941 atggcgcgaa cacaggcggc gcaggaataa tcgccgtgac cagcgacatc accggcactc
    24001 cggcgttcga caccgatggc tatcatctga ccgctggctc ggccgcgctg aatcgaggag
    24061 tcgacaccgg gatgagtcgg gatatcgacg gtgaatcgcg gcccatgggc gctgcgcctg
    24121 accttggagc agacgagctc cccatcggcc tgctcgtgac taagcaggtt acccccagcg
    24181 tagtcccggc cggatcgcca gtgacctata ccgtccgcgt cacgaacaca ggcgccgtga
    24241 gcctcacagc gacgatcagc gatctcttgc caagtcaagt tgtacccacc ggcatcctga
    24301 cctggacgcc cacaatcacg gctcctggcg gtgtgtgggt gcagcaattt agcgttaccg
    24361 ttgccgtggg gtacagcggc acactgacca acgttgttca ggtgtcgagt caggaaggcg
    24421 cttccggcgt gttcactgcg acttcatccg ccaccgtgat gccttcattc ataatgagca
    24481 agtgggctga tcccgaccca gtcgaagcgg gcttgccatt gacttacacg ctggtcatca
    24541 ccaatgccgg ggatgccgag ctacacgcga ctgtcaccga taccttacct ggtcaggtca
    24601 cgccatcggg cgttatcacc tggacgcccc tgatcacggc cccgggtggc gtgtggatac
    24661 agcagttggt ggtgactgtg gtggagggct atagcgggac gttgaccaac accctgcacg
    24721 ccacgacggt tgagggggtc acaggcgtgt acacactgac ctcgcgcgca atcgcgccgt
    24781 acctcgtcta tctgccggtt gtgctgcgag cgtctccctg agccagtcgc aatcacagac
    24841 ggtctggcaa cgaaccgttg ctccggcccg ccgctaaccg ttttgtacca gggataagag
    24901 caggatgata accgcgatca ggatgagcgt cagcagcgtg ccgattcgca ggaagtcgtt
    24961 gaacttgtag ccccccggcc ccatgaccag cacgttgact gggtgaccga gcggcgtcat
    25021 gaatgccagc gaacaaccat aggccactgc caatgcaagt ccgcgcggat tgacctggag
    25081 cgtttctgcg gcgtgaatcg cgatcggagc gaggagcacg gcgaccgccg ggccgctcgt
    25141 cacctgcgtg agcagtgtcg cgatcgccag cagcccaccg gcgacagcca gcggccccag
    25201 accacccagc agcgcgacca gtcgctcgcc cagcacactc gccacgcccg tcttcgtcat
    25261 cgcgatcccc actggcagca tcgcggcgat caggaaaatg cttttccatt cgatcgaccg
    25321 gtacgcttcg tccatggtca gcgcgcccgt gagcaccatc gcc
//
LOCUS       DBNHXB010000176        11880 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2389317, whole genome shotgun sequence.
ACCESSION   DBNHXB010000176 DBNHXB010000000
VERSION     DBNHXB010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11880
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2389317"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1362
                     /locus_tag="AC162G_06995"
     CDS             169..1362
                     /locus_tag="AC162G_06995"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR5075592.1"
                     /translation="MDTHCYQRRLERCSQLMTDAGLDALLLTKPSNMAYLTGDGRLCA
                     YAMITQKGEVALGVPVTDIEDVKQLARFDHIVGFDDEVGMIHSIAHYFEHFGMREGTV
                     GLEYTFLTQSMMGMFTHPHAKPEKIEVRDCTHILSELRMVKDAEEIERIRAAAKVADI
                     GMAAAAKAVKPGVTESQVAAEAEYAMRQAGAQEFYRTYVSSGPRTNIAHGLPTSRRLQ
                     KGDLVMIDLHPIVDGYSADMCRTVSVGKPAADQQAAYDLFLKAQQATIAKVKAGVSMV
                     ELEETIHGALKEGGHGGHIFGPPIHGLGIEFEEAPLPPGHAFFHGEKAPPPLEVNVVI
                     TVGNCGLYTGPWGVRVEDTVAVGADGSAVLTTFPRTLEPAQARRPRPSRSRKAVKRPS
                     RSRSR"
     gene            1521..2651
                     /locus_tag="AC162G_07000"
     CDS             1521..2651
                     /locus_tag="AC162G_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014164.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR5075593.1"
                     /translation="MLADKIVEMLSVSPGQVIAIWASTHSLDLVEALAVRIRARGAFW
                     TLRLTMESLLRRIGQSVPEPYLASVPEHELRWLADVSAIVEVRDHGGHILEVPLVRRR
                     ALAAEWIALIDEARRRGCRRIQVVNPTPALASAYGVPVEDLRQSYWRAVNVDYAALDR
                     QQDRVAARLARARLVHVASALGTDLHLRIDDRPVHQDTDSLPRGEVYVAPHEDSAEGV
                     AVIDRAFVAGKPIERLRLVFRKGRVVDASAADPSAAHLFRELLSASAGDKDVIAELAI
                     GLNPGAAEPIGDVMLDEKIAGSCHIAIGMNTHFGGRNQSNLHLDLVMLRPTVRTDGEV
                     FVDNGATLPSTPFSERIGERATRTGGRHEPDSHSNRESHARF"
     gene            2605..3537
                     /locus_tag="AC162G_07005"
     CDS             2605..3537
                     /locus_tag="AC162G_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009630296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5075594.1"
                     /translation="MNQTAIRTENLTRDFETVRAVDGLSLEVPAGIIFGFLGPNGSGK
                     TTTIRLLLGLLEPTAGWAEVLNWDTRTQADEVRLRSGALLEHNGLYERLSAEANLEFY
                     GRVWRLPPADRRARIQELLTHLGLWERRKEVVKNWSRGMKQKLAVARALLHHPALLFF
                     DEPTAGLDPVAAAALRDDLAGLAAREGVTVFLTTHNLAEAEKLCNQVGVIRQGKLLAV
                     GHPDELRSHRGAPQAEIVGRGFDENLLAALRARPDVAAAEVRNGRLMIDLRGEVDVAP
                     LVSLIVSAGAQIEEVRKGKASLEEVFLTLMEEEK"
     gene            3534..4268
                     /locus_tag="AC162G_07010"
     CDS             3534..4268
                     /locus_tag="AC162G_07010"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5075595.1"
                     /translation="MIADVVTVMWKDARELLFGGGTRRGWLSLLIIIGVFGVFLPLQF
                     GRGWLETPLMLVYWAWIPLFMVSSSVSDSFAGERERHTLETLLASRLSDRAILFGKFC
                     TAVCYGWGLTVVSLLVGLVTINVAHGRGELVWYPALIFWGAVVMSLLMSSLTASIGVL
                     VSLRAATVQQAAQRLSLGVMIVVFAPILIVQVLPVEWQARVVQALQNTDFTGLAVIVV
                     AILVALNALFLTAAMARFRRARLILD"
     gene            4451..4696
                     /locus_tag="AC162G_07015"
     CDS             4451..4696
                     /locus_tag="AC162G_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR5075596.1"
                     /translation="MAKGRIIVDEERCKGCELCTTVCPKDLIRMADTFNAKGYRPAAL
                     IDPEGSCTGCAICAVICPDAAITVYRFAKAPGLGVPA"
     gene            4794..5897
                     /gene="vorB"
                     /locus_tag="AC162G_07020"
     CDS             4794..5897
                     /gene="vorB"
                     /locus_tag="AC162G_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HIR5075597.1"
                     /translation="MPKELLKGNEAIAEAAIRAGCVAYFGYPITPQTELLEHMARRMP
                     ELGRAFVQAESEVAAINMCYGAACSGVRVMTSSSSPGVSLMQEGLSYIAGSEVPVVLV
                     DVMRGGPGLGNIAPSQGDYNQVVRAAGHGDFRSIVLAPATVQEAIDLTAEAFNLAEKY
                     RHIAILLLDGNLGQMMEPAELPPMMPVDGRRPDWALTGAAGRPHNIISSIYLQPEELE
                     RVNQRLQAKIERIRAEEVRYQEYMLEGADLIVVAYGTAGRIAQSAIKMARQQGIPVGL
                     LRPISLFPFPEDRISQLADEARGFLVVEMSAGQMFEDVRLAVAGQAPVRFFGRMGGMV
                     PMPEEILAAIVKLDQATSPQIALRNVAHARSGS"
     gene            5917..6699
                     /locus_tag="AC162G_07025"
     CDS             5917..6699
                     /locus_tag="AC162G_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5075598.1"
                     /translation="MVVQDTQSATTHVEAIEELIYRRPASLTVAHTHYCPGCTHGVAH
                     RLVAEVIDELGIREKTIGVAPVGCAVFAYNYFNCDFAEAAHGRAPAMATGIKRVQPDK
                     VVFTYQGDGDLAAIGTAEIVHAAARGENITVVFINNAIYGMTGGQMAPTTLPNMKTTS
                     TPTGRDVELAGFPIRMAELLSRLDGASYVVRRSLHNPAEIRKAKKAIRSAFENQMQGC
                     GFSMVELLSSCPVNWNMQPVEALKWIETHMIPIFPLGDYKSL"
     gene            6773..7333
                     /locus_tag="AC162G_07030"
     CDS             6773..7333
                     /locus_tag="AC162G_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR5075599.1"
                     /translation="MQQEIIISGFGGQGALFAGQLLAYAAMEEGRHVTWLPSYGPEMR
                     GGTAHCTVIVSDEEIGSPVVQRPSAAIVLNNPSMEKYQKLVKEHGYLLINRSLINLPT
                     TRADVHAVDVPASDEAVALGNPKLLSVVMLGALIACVEVVSLDALEGALESHLPERHR
                     QLLDLNKAALRRGMELARQQMVAQTV"
     gene            7382..8632
                     /locus_tag="AC162G_07035"
     CDS             7382..8632
                     /locus_tag="AC162G_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR5075600.1"
                     /translation="MTTPWMQRYAQRTQRMSSSMIRELLKFTAQPDIISFAGGLPAPE
                     LFPIEETRAATDVVLSEMGQQALQYSPTEGYVPLREMIVRHMARYGIVVDVDNVLITS
                     GSQQALDLIGKILINPGDKILTEEPTYLGAIQAFTMYGAEYVTVPIDEDGMQVSKLEQ
                     ALRAGPKFMYILPNFQNPAGVTLSLERRRDIIAFADQYGIPIVEDDPYGQLRYEGEHI
                     KPLVVLDAECLECENNGKYGGNVIYLSTFSKTLAPGLRLGWVVAPKEVIRQIVLAKQG
                     TDLHTSTFDQMVAYEVARGGFIDRHVRHIREVYHHRRDVMLAALERAFPDPDMGVRWT
                     RPKGGLFLWVALPEWMDAAELLKEAVKDKVAFVPGIAFHPNGGGHNTMRLNFSNARDE
                     MIEEGIARLGRAVLKQIRAQRVAV"
     gene            complement(8726..9643)
                     /locus_tag="AC162G_07040"
     CDS             complement(8726..9643)
                     /locus_tag="AC162G_07040"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR5075601.1"
                     /translation="MRIVLIGQAAFGEKVLEAILNKGEEVVGVFCPPDRTDKPDPLRQ
                     LAERQDIPVYPSRQMRDPAVYDRYVKLRPDLNVMAFVTEVVPESILNTPRHGTIQYHP
                     SLLPKHRGGSAINWAVINGETRTGLTIFWPDRGIDTGPILLQKEVAIAPDDTTGSLYF
                     NKLFPLGVEAIVEAIEMVRNGCAPRIAQDESQATYEPLCTQERVAIHWEWPAVQVYNL
                     IRGSNPQPGAVTAFRGKRLKIFDCQRQVLPGPAGSGEVLAIGEEGFWVAAGDGSILVK
                     RVQPEGGAKIKAPEFVAASGLRVGDRLGT"
     gene            complement(9703..11301)
                     /gene="lipA"
                     /locus_tag="AC162G_07045"
     CDS             complement(9703..11301)
                     /gene="lipA"
                     /locus_tag="AC162G_07045"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR5075602.1"
                     /translation="MTRHTVSVLKLGTLEYRWALQLQERLVAARQQEVVGDLLLLVEH
                     RPVITFGRGGGAEDLRVPEATLHRQGVSLIQTDRGGRATYHGPGQLVVYPILKLHTPA
                     HPYDPHTYLWRLEETVIACLARFGIVAGRQEPHPGVWVGEDKIAAVGIALQRGVTYHG
                     LALNVDPPLEHFQHITPCGLADKGVTSMAQVLDRPVSLAAAEAAILEAFAHVFGVDLE
                     FGFQKAPWLIALAPLGERVEQLYSLFQGLRLHTVCEEALCPNIGECWGEGTATFMLLG
                     DICTRHCRFCAVIPGRPSPPDPLEPVRVAEAAARLGLHHVVITMVARDDLPDGGAAQV
                     ATTIAEVRRRCTDATVEVLISDFGGSVSALEQVISARPDVLAHNVETVPRLYPVIQPR
                     KDYQRTQGVLAWAKRAGLIAKSGMILGMGETRGEIIQVMKDLRRAGCDILTLGQYLQP
                     TANQRPVVEYIHPAEFGWYREVGLSLGFRAVVADPLVRSSYQAGSLLRQVGSAVPLAT
                     DWVNSRIEPVLSCARYVQQSPPRT"
     gene            complement(11298..11834)
                     /locus_tag="AC162G_07050"
     CDS             complement(11298..11834)
                     /locus_tag="AC162G_07050"
                     /inference="COORDINATES: protein motif:HMM:NF014860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5075603.1"
                     /translation="MGIRVEGGMVMVDDHQQTAQPMVYAAGDVTGGWMLSQFARAQGL
                     VAAENALGEKTTLEPRLIPRVYHTTPEVGAVGFTEPEAQAQGEIVVGRSDFGFNARAL
                     TLGQPLGLVKVLAQKPHGKLIGVHILGPHATELIAQAAMALRLEALAEDLAGMSLGHP
                     TLGESLVEAAQDAVRQLG"
BASE COUNT         2212 a         3747 c         3786 g         2135 t
ORIGIN      
        1 cattcgccca ctgacggcag acgagacgag ccggcagcga caggccatcc gatcggcact
       61 gcgcttcggg cggggcggcc ccatcgtgtc gaagtacgat attcgcgagt tcgtctatta
      121 gaacgtaggg gcgacccttg cggtcgcccg attagaagga ggtcaaagat ggacacccat
      181 tgctaccagc gtcgcctgga acggtgcagc caattgatga cagatgccgg gttagacgcg
      241 ctgctgctca ccaagccgtc caacatggcc tatctcacgg gtgacgggcg gctgtgtgcg
      301 tacgccatga tcacgcagaa aggggaagtc gccctgggcg tgcccgtcac cgacatcgag
      361 gacgtgaagc agttggctcg cttcgatcac atcgtcggct tcgacgatga ggtggggatg
      421 attcactcca tcgctcacta cttcgagcat tttggcatgc gcgaagggac cgtgggcctg
      481 gagtacacgt tcctcacgca gtccatgatg ggcatgttca cgcaccccca cgccaagccg
      541 gagaaaatcg aagtcagaga ctgcacccac atcttgtccg agctgcgcat ggtgaaggac
      601 gccgaggaga tcgagcgcat ccgggctgca gccaaagtcg cggacatcgg gatggccgcg
      661 gccgctaaag ccgtgaagcc gggcgtcacc gaaagccagg tcgccgccga agccgagtac
      721 gccatgcgcc aggcaggagc acaagaattc tatcggactt acgtgtcatc cggaccgcgc
      781 accaatattg cacacgggct gcccacgagc cgtcgattgc agaaaggcga tctggtgatg
      841 atcgatctcc accctatcgt cgacggctac agcgccgata tgtgtcgtac cgtcagcgtg
      901 ggcaaaccgg ctgccgatca gcaagccgcc tacgacctgt tcctgaaagc gcagcaggcg
      961 acgatcgcga aggtgaaggc aggcgtcagc atggtcgagc tggaggagac gatccacggc
     1021 gccctcaagg aaggggggca cggcgggcac atcttcgggc cacccatcca cgggttgggc
     1081 atcgagtttg aagaagcgcc actgccgccg ggccacgcct tcttccacgg tgagaaggcg
     1141 ccgcctccgt tggaggtgaa cgtggtcatc acggtgggca actgtggact gtataccggc
     1201 ccgtggggtg tgcgcgtgga ggatacggtc gcggtcggcg cagacggaag tgcggtgctc
     1261 acaactttcc cacgcacgtt ggaaccggcg caagcgcgcc gtcctcgccc atctcggagc
     1321 cgcaaagccg tcaagcgccc ttcgaggagc cgctcgagat aggaaatggt ctcggaaggt
     1381 ggtagtaacg actttagtcg tttcatgagc gactaaagtc gccactacca gccatctcga
     1441 gatagtctct cagctctgag cggcagaatg caggagtcac tacgggaaac tcacataacc
     1501 cagacggctt tgacgtcgag gtgctggccg acaagatagt agagatgctg tccgtgtcgc
     1561 ccggtcaggt gatcgcgatc tgggcgagca cccattcgct cgatcttgtc gaagcgctcg
     1621 ccgttcgcat ccgagcgcgt ggcgcgttct ggacgttgcg cctaacgatg gaatcactgt
     1681 tgcgccggat cggtcaaagc gtgcccgaac cctacctcgc ctcggtaccc gaacacgaac
     1741 tgcgctggct cgcggatgtg tcggcgatcg tcgaagtccg cgatcacggc ggccacatcc
     1801 tggaagttcc gctcgtgcgc cgccgcgccc tggccgccga atggatcgcg ctgatcgacg
     1861 aagccagacg acgcggctgc cgccgtatcc aggtcgtgaa ccccacacca gccctcgcgt
     1921 ccgcctacgg cgtacccgtc gaagacttgc ggcagagcta ctggcgagcc gtaaacgtcg
     1981 actacgccgc gctcgaccgg cagcaagacc gggtcgccgc ccggctggca cgtgcccgcc
     2041 tcgtgcacgt ggcctcggcg ctgggcacag acctgcatct caggattgac gatcggcccg
     2101 ttcatcagga tacggacagt ctgccgcgcg gcgaggtgta cgtcgccccg cacgaggact
     2161 cggccgaggg tgtggccgtg atcgatcggg cgttcgtcgc cggaaagccc atcgaacggc
     2221 tacggctggt cttccggaag ggtcgcgtcg tcgatgcgag cgcagccgat ccgagcgcag
     2281 ctcatctgtt cagagagcta ttgagcgcct ccgccggcga caaggatgtg attgcggaat
     2341 tggccatcgg gctgaacccg ggcgccgccg agccgattgg cgatgtcatg ctcgacgaga
     2401 aaatcgccgg aagttgccac attgccatcg gcatgaacac gcatttcggc ggtagaaatc
     2461 agtcgaatct gcatctggat ttggtcatgt tgcgcccgac cgttcggacg gatggcgaag
     2521 tgtttgtgga caatggcgcg accttgccat ctacgccgtt ctcggagaga attggagagc
     2581 gagcaacacg gacgggggga cggcatgaac cagacagcca ttcgaaccga gaatctcacg
     2641 cgcgattttg aaaccgtgcg cgccgtggac ggcctttcac tggaagtgcc agccgggatc
     2701 atcttcggct tcctcggccc gaacggctca ggcaagacga cgacgattcg cctgctgctc
     2761 ggcctgctcg agccgacggc cggctgggcc gaagtgctga attgggatac acgcacacag
     2821 gcggacgagg ttcgcctgcg ttccggcgcg ctgctcgagc acaacggctt gtatgagcgg
     2881 ctgagcgccg aggccaacct ggaattctac gggcgggtgt ggcgcttgcc gccggccgac
     2941 cggcgcgcgc gcattcagga actgctcacc cacctgggcc tgtgggagcg gcgcaaggaa
     3001 gtcgtgaaaa actggagccg cggcatgaag cagaaactcg cggtggcgcg cgcgctgctg
     3061 catcaccccg cgctcctctt cttcgacgag ccgacggccg gactcgatcc ggtcgccgcc
     3121 gccgcgctgc gcgacgatct cgccgggctg gccgcgcgcg agggcgtgac ggtgtttctg
     3181 acgacgcaca atctggcgga agcggaaaag ttgtgtaacc aggtcggcgt gattcgccag
     3241 gggaagctgt tggccgtcgg ccatcccgac gagcttcgtt cgcatcgtgg tgcgcctcag
     3301 gcagaaatcg tcggacgggg gttcgacgag aacctgctgg cggcgctccg ggcgcggccg
     3361 gatgtggcgg cggccgaggt gcgcaatgga cggctgatga tcgacctgcg cggcgaagtc
     3421 gacgtcgcgc cgctcgtcag cctgatcgtc agcgccggcg cgcagatcga agaagtccgc
     3481 aagggaaagg ccagcctgga ggaagtgttc ctgacgctga tggaggaaga gaaatgatcg
     3541 ccgacgtcgt gacggtgatg tggaaagatg cccgggagtt gctgttcggc ggcggcacac
     3601 gccgaggctg gctcagcctg ctgatcatta tcggcgtgtt cggcgtcttc ctcccgctgc
     3661 agttcgggcg tggctggctc gagacgccgt tgatgttggt ctactgggcg tggattccac
     3721 tgttcatggt cagcagcagc gtgagcgact cgttcgccgg cgagcgcgaa cggcacacgt
     3781 tggagacctt gctggccagc cggctgtcag accgcgccat cctcttcggg aagttctgca
     3841 ccgcggtgtg ctacgggtgg ggcctgacgg tcgtcagcct gctcgttggg ctggtgacga
     3901 tcaacgtggc gcacgggcga ggcgagcttg tgtggtaccc ggcgctgata ttttggggcg
     3961 ccgtcgtcat gagcctgctg atgtcatcgc tcacggcgag catcggcgtg ctggtttcct
     4021 tgcgcgcggc gacggtgcag caggccgcgc agcggctgag cctcggagtg atgatcgtcg
     4081 ttttcgcgcc gatcctgatt gtgcaggtgc tgccggtcga gtggcaggcg cgcgtcgtcc
     4141 aggcattgca gaacaccgat ttcaccggct tagcggtaat cgtggttgcc atcctggtcg
     4201 ccctgaatgc gctcttcctt acggcggcga tggcccgctt ccggcgcgcg cggctgatcc
     4261 tcgactgaga tgcctcaaac ccgctcagcc cccgattcgc cttgaaaatc ggccatcttt
     4321 cttccggttt tgtcataagt ttcgtgagaa tcatcacatc ccaaaatgac atcttgcact
     4381 gtgacctgcc ctccgatcgg tctatcctga atgctggata cataagccgc gcgacgccgg
     4441 aggaggcgct atggcaaagg gtcgtatcat cgtcgacgag gaacgctgca agggctgcga
     4501 gctatgcacc accgtctgcc cgaaggactt gatccgcatg gccgacacgt tcaatgcgaa
     4561 aggctaccgg ccggcggcgc tgatcgatcc tgaagggagc tgcaccggct gcgccatctg
     4621 cgcggtgatc tgcccggacg ccgcgatcac ggtttatcgc ttcgccaaag cccccggact
     4681 gggtgtgccg gcttagtgtc tatccgacaa cccgtagcgt agggccttgt gcccgccgcc
     4741 cacaaggggc taggctacgt tttcgcatag gctcttagtc ctcaggagat gtcatgccga
     4801 aggaactgct caaggggaac gaagctattg ccgaagccgc cattcgcgcc ggctgcgtgg
     4861 cctattttgg ctacccgatc acgccccaga ccgagttgct ggaacatatg gccaggcgca
     4921 tgcccgagct ggggcgcgcg ttcgtgcagg ccgagagcga ggtcgccgca atcaacatgt
     4981 gctacggcgc ggcctgcagc ggcgtgcgcg tcatgacctc gtcgtccagc cccggcgtca
     5041 gcctgatgca ggagggcctg tcgtacatcg ccggctcgga agtgcccgtc gtgctggtcg
     5101 acgtgatgcg cggcgggccg ggcctgggca acatcgcgcc gagccagggc gattacaacc
     5161 aggtcgtgag ggcggccggc cacggcgact tccgatccat cgtgctcgcc ccggccaccg
     5221 tccaggaagc gatcgatttg acggccgaag ccttcaacct ggccgaaaaa taccggcaca
     5281 ttgccatcct cctcctcgac ggcaatctgg gccagatgat ggaacccgcc gagttgccgc
     5341 cgatgatgcc ggttgacggc aggcgccccg actgggcgct gacaggcgcc gccgggcggc
     5401 cccacaacat catctcctcg atctacttgc agcccgaaga gctagagcgt gtcaaccaga
     5461 gactgcaggc caagatcgag cgcatccggg ccgaggaagt ccgctaccag gagtacatgc
     5521 tcgaaggcgc cgacctgatc gtcgtggctt acggcaccgc cgggcgcatc gcgcaaagcg
     5581 cgatcaagat ggcgcggcag cagggcatcc cggtcgggct gctccgtccg atcagcctct
     5641 tccctttccc ggaggatcgc atctcacaac tggccgacga ggcgcgcggc ttccttgttg
     5701 tcgagatgag tgccgggcaa atgttcgagg atgtgcggct cgccgtagcc ggccaggcgc
     5761 cggtgcggtt cttcggccgc atgggcggca tggtgcctat gcccgaagag atattggccg
     5821 ccatcgtcaa gctcgaccag gctacgtcgc cgcagatagc gctgcgcaac gtcgcgcacg
     5881 cgcgcagcgg cagttgattt cgcgagggag gacatcatgg ttgtccaaga tacgcagagc
     5941 gcgacgacac atgtcgaagc gatcgaggag ctgatctacc ggcggccggc cagcctgacg
     6001 gtggcgcaca cgcattactg ccccggctgc acgcacggcg tcgcgcaccg cctggtcgcc
     6061 gaagtgatcg acgagctggg cattcgcgag aagacgatcg gcgtcgcgcc ggtcggctgc
     6121 gccgtgttcg cctataacta cttcaactgc gacttcgccg aggcggcgca cggccgcgcc
     6181 ccggcgatgg cgaccggcat caagcgcgtg cagccggata aggtcgtgtt cacctaccag
     6241 ggcgacggtg acctggcggc catcggcacc gccgagatcg tccacgcggc ggcgcgcggc
     6301 gagaacatca ccgtggtctt catcaacaat gcgatctacg gcatgaccgg cggccagatg
     6361 gcgccgacga cgctgcccaa catgaagacg acgtccaccc cgaccgggcg cgacgtggag
     6421 ctggccggct tcccgattcg catggccgag ctgctgtcca ggctggacgg cgcttcgtac
     6481 gtcgtgcggc gcagcctgca caacccggcc gagatccgca aggcaaaaaa ggcgattcgc
     6541 agcgcgttcg agaatcaaat gcagggctgc ggcttctcga tggtcgagct gttgtcgtcg
     6601 tgcccggtca actggaatat gcagccggtc gaggcgctca agtggatcga gacgcacatg
     6661 ataccgatct tcccgctggg cgactataaa tccctttagt tcctccagtt ccctcaggcc
     6721 ctttcgcaag aagcactcaa ggaactgaag gaactcctgg cacggagatg atatgcaaca
     6781 ggagatcatt atcagcggtt tcggcggcca gggtgcgctg ttcgccggtc agttgctggc
     6841 ttacgcggcg atggaggagg gcaggcacgt cacctggctc ccttcgtatg gcccggagat
     6901 gcgcggcggc acggcgcact gcacggtgat cgtctcggac gaggaaatcg gatcgccggt
     6961 ggtgcagcgg cccagtgcgg ccatcgtctt gaacaacccg tcgatggaga agtatcagaa
     7021 gctggtgaag gagcacggct atctcctgat caatcgctcg ttgatcaacc tcccgaccac
     7081 gcgggcggac gtccacgccg tcgacgtccc ggcgagcgac gaggccgtcg cgcttggcaa
     7141 ccccaagctg ctgagcgtcg tcatgctcgg cgcgctgatc gcctgcgtcg aggtcgtctc
     7201 gctcgacgcg ctcgagggcg cgctcgaatc gcacctgccg gagcggcacc gccaactgct
     7261 cgacctcaac aaggccgcgc tgcggcgggg tatggagctt gcccggcagc agatggtggc
     7321 acagacggtt tagttcagaa acccggtttc tacaagaaac cgggtttttg aaggagctta
     7381 catgactaca ccttggatgc aacgctatgc gcagcgcacg cagcgcatga gcagttctat
     7441 gatccgtgag ctgctcaagt tcaccgccca gccggacatc atttcgttcg cgggcggttt
     7501 gcctgcgccc gagctgtttc cgatcgagga gaccagggcg gcgaccgacg tcgtcttgtc
     7561 cgagatgggg cagcaggccc tgcaatacag cccgaccgag ggttacgtcc ccctgcgcga
     7621 gatgatcgtt cgccacatgg cgcgctacgg catcgtcgtg gatgtcgaca acgtcctgat
     7681 cacgtccggc tcgcagcagg cgctcgatct gatcgggaag atcctgatca accctggcga
     7741 caagattctg accgaagagc cgacgtacct gggcgcgatc caggcgttca cgatgtacgg
     7801 cgccgagtac gtgaccgtgc cgatcgacga agacggcatg caggtgagca agctcgagca
     7861 ggcgctgcgc gccggcccga agttcatgta catcctaccc aactttcaga acccggccgg
     7921 cgtcacgctg tcgctggagc gccggcgtga catcatcgcc ttcgccgatc aatacggcat
     7981 ccccatcgtc gaagacgatc cgtacggcca gttgcgctac gaaggcgagc acatcaagcc
     8041 gctcgtcgtg ctcgacgccg agtgcctgga gtgtgagaac aacggcaagt acggcggcaa
     8101 cgtcatctac ctgagcacgt tcagcaagac gctcgcgccg ggcttgcggc tgggctgggt
     8161 tgtcgcgccg aaggaggtca tccgccagat cgtgctggcc aagcagggca ccgacctgca
     8221 cacgagcacg ttcgaccaaa tggtggcgta cgaggtcgcg cgcggcggct tcatcgaccg
     8281 gcacgtgcgc cacattcgcg aggtatatca ccaccgccgc gacgtgatgc tggccgcgct
     8341 cgagcgcgct ttccccgatc cggacatggg cgtgcgctgg acccgcccca agggcggctt
     8401 gttcctgtgg gtcgccctgc ccgagtggat ggatgcggcc gagctcctca aggaggccgt
     8461 gaaggacaag gtcgcgtttg tcccggggat cgcgttccac ccgaacggcg gcggtcacaa
     8521 cacgatgcgc ctgaacttca gcaacgcccg ggacgagatg atcgaggagg gtattgcgcg
     8581 gctgggacgt gcggtactga agcagatccg cgcgcagcgc gtggcggtct aactgcagtc
     8641 atcagtgatc agttgtcagt aatcagtagt cattaaccac tgattactga tcactgatta
     8701 ctgatcactg aacactgccc actctctacg ttcccagtcg atcgccaact cttagaccac
     8761 tcgctgccac gaactctggc gccttgatct tggctccacc ctccggctgc actcgcttga
     8821 cgaggatcga gccgtcgccc gccgcgaccc aaaagccttc ctcgccgatc gccaacacct
     8881 cgcctgagcc tgccgggccg ggcagcactt ggcgctgaca atcaaagatc ttgagccgtt
     8941 tgccgcgaaa agccgtcact gcccccggct gtgggttcga acctcggatc aggttgtaga
     9001 cctgcacggc gggccattcc caatggatgg ccacgcgctc ctgggtgcag agcggctcgt
     9061 aagtggcttg agactcatct tgggcgatgc ggggcgcgca cccgttcctc accatttcga
     9121 tggcttcgac aatggcctcc acgccgaggg ggaacagctt attgaaatag agcgagcctg
     9181 ttgtgtcgtc tggagcgatg gccacttctt tctgcaatag gatcgggccg gtgtcgatgc
     9241 cgcgatcggg ccagaagatg gtcaggccgg tcctggtttc tccgttgatc acggcccagt
     9301 tgatcgcgct gccgccgcga tgtttcggga gcagcgaggg atgatattgg atcgtgccat
     9361 gccgtggagt gttgaggata ctttcgggga cgacttctgt gacgaaggcc atcacattga
     9421 gatcgggcct caacttcacg tagcggtcat atacggccgg gtcgcgcatc tgcctggatg
     9481 gatacaccgg tatgtcctgg cgctcggcga gttgtcgcag cgggtcaggc ttgtccgtgc
     9541 gatcgggtgg gcagaagaca cccacgacct cttcgccttt attgagaatc gcttccagca
     9601 ccttctcacc gaaagccgcc tgaccgatca ggacgatccg catacccgcc tcctcattgc
     9661 ttctttgttc cggccaggtt ctcgaagtca accggcgtga gattacgttc gcggtggaga
     9721 ctgctgaacg tacctggcgc aagagagaac cggttcgatc cgtgaattca cccaatccgt
     9781 tgccagaggg actgctgaac ctacctggcg caagagagaa ccggcctgat aggaactgcg
     9841 tacgaggggg tcagccacaa cggcccggaa gcccaggctc aacccgacct cccggtacca
     9901 gccaaactct gccggatgaa tgtactcgac cactggccgc tggttcgccg tcggttgcag
     9961 gtattgtccc agggtcagga tgtcacaccc cgcccgccgc aggtccttca tcacttggat
    10021 gatctcgcct cgcgtctctc ccatgcctag aatcattccc gacttggcta tcaggccggc
    10081 gcgcttggcc catgccaaca caccttgcgt ccgctgataa tccttccgag gctgaatcac
    10141 tggatagagt cgagggacgg tctccacgtt gtgagctaaa acatcgggcc gagcggaaat
    10201 cacctgttcc aaggccgaca ccgatccccc aaagtctgag atcagcactt cgaccgtcgc
    10261 gtcggtgcag cggcggcgca cctcggcgat cgtggtggca acctgtgcgg cgcccccgtc
    10321 gggcaaatcg tccctggcaa ccatggtgat gaccacgtga tgcaatccca gccgggctgc
    10381 cgcctccgcc acacgcacgg gctccagcgg gtctggcggc gacggtcgcc cgggtatcac
    10441 cgcacaaaaa cgacaatgcc gcgtgcaaat gtcacccaag agcataaatg tcgccgtccc
    10501 ctccccccag cactcgccga tgttagggca cagggcctcc tcgcacacgg tatgcagccg
    10561 aaggccctga aatagactat acaattgctc cactctctcg cccagagggg ccagggcgat
    10621 cagccagggg gccttctgaa agccaaactc gaggtccact ccgaagacat gagcgaaggc
    10681 ttcgagaatg gccgcctccg ccgctgccag actcaccggc cgatcgagga cctgggccat
    10741 ggacgtaacc cctttgtcgg ccagaccgca gggggtgatg tgctggaagt gctccaaggg
    10801 gggatccaca ttaagcgcca agccgtgata ggtcacgcct ctttgcagcg cgatgcccac
    10861 ggcagcaatt ttgtcctccc caacccacac gccgggatgg ggttcctgcc gccccgccac
    10921 gatgccgaat cgggccaggc aggcaatgac ggtctcctcc aaccgccaca agtaggtgtg
    10981 gggatcgtat gggtgggccg gcgtatgtag tttgaggatg ggataaacca ccagttggcc
    11041 cgggccgtgg taggtggccc ggccgccccg gtctgtctgg atgaggctga ctccctggcg
    11101 gtgcagggtc gcctccggaa cccgtaaatc ctcagcgccg ccgccccgac cgaaggtgat
    11161 gacgggacga tgctcaacca acagcagcag gtcgccaacg acctcctgtt gcctggccgc
    11221 taccaaccgt tcctggagtt gcaaggccca gcgatattca agggtgccta gcttcaggac
    11281 cgaaacggtg tggcgtgtca tccgagttgt ctcacggcat cctgggcggc ttccaccagc
    11341 gattcgccca gcgtgggatg gccgagggac atcccggcca ggtcctcggc cagggcttct
    11401 aagcgcaagg ccatcgctgc ctgggcgatc agttctgtcg cgtgggggcc gagaatatgc
    11461 acgccgatca gtttcccgtg aggtttctgg gccaagacct tcaccagccc gagcggttgc
    11521 cctagggtca gtgcacgagc attgaaaccg aagtctgatc gcccgacgac gatttctccc
    11581 tgagcttgcg cctccggttc agtgaacccc accgccccca cctccggcgt ggtgtgataa
    11641 acgcggggga tcagcctggg ctcgagcgtc gtcttctcac cgagggcatt ttcggcggct
    11701 accagccctt gagcgcgggc gaactgggag agcatccagc caccggtcac gtccccggcc
    11761 gcataaacca tcggctgcgc cgtctgctga tggtcgtcca ccatcaccat ccccccttct
    11821 actcgaatcc ccaggcctgc cctatccgtg acatctgtgc tatccgtggt tggagtcgaa
//
LOCUS       DBNHXB010000177        16985 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3059952, whole genome shotgun sequence.
ACCESSION   DBNHXB010000177 DBNHXB010000000
VERSION     DBNHXB010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 16985)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16985)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16985
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3059952"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="AC162G_07055"
     CDS             complement(<1..483)
                     /locus_tag="AC162G_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075604.1"
                     /translation="MPSAEGYHNIPTGGHMNVNRLSDWRGHLARALALILACAISVQT
                     TRAESFASNQPSTRLPSHATNPHAPPRTDTLVLPGGEDITEYGLAATTRPARLPDGLH
                     APGGRTLMLPVAPLRLARARAPVTRQDQSASPSISKSASASTVAFGDVFTYTVVFTVP
                     A"
     gene            complement(602..1711)
                     /locus_tag="AC162G_07060"
     CDS             complement(602..1711)
                     /locus_tag="AC162G_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5075605.1"
                     /translation="MKIRFLLDLAAALAACAAPTPAFDEHLAAGRAALDAGDTAKAIA
                     ELETAVGLDPMRAEAQVLLGQAYYTAGRSQEARERLQTGLRQDPNNAAAQYLLGDLSV
                     DAGRLLDAATAYAAAVKIDPGARTAHPKIEDSVEAALQAGQDAASAGQWQQAIPYLEA
                     ALALNPGLTEARFALGNSRFNLGLEAELAGDRATAIGHYAAAQADYEAVLELDPEYAP
                     ALTNLGVVAYQTGDLERAVSLFQQALAIDPGDTDTRYQLGAAYLNMDKRDAARREFER
                     ALEMEPDFPQALIGLGNVYLLDKEYDRAVETLQRVVELMPDSPEALFALGKTYAEMGR
                     CPDAVPVLQKFGQLNPAEPFKSDAGKILQTCAGSP"
     gene            complement(1708..2379)
                     /locus_tag="AC162G_07065"
     CDS             complement(1708..2379)
                     /locus_tag="AC162G_07065"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5075606.1"
                     /translation="MEITWLGHSCFRLRSRAATVVTDPYGKDLGLTLPRVRADIVTVS
                     HAADDHDYVKGVKGDFKVLSGPGEYEVSGAFITGLVLGERKTGGKSSKSGATATDRNT
                     VFLVELDGLTVCHLGDLNTVPTQAMMEESLSEVDVLLVPVGGGESLNASQAAEVVSLL
                     EPHIVIPMHYKFRGSDLKLDPVSKFLKEMGLDKVEEQEMLKLDRSGLPDETQVVLLEL
                     KSKDE"
     gene            2581..4422
                     /locus_tag="AC162G_07070"
     CDS             2581..4422
                     /locus_tag="AC162G_07070"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR5075607.1"
                     /translation="MSTDRRIAELTTLNEIAATLNESLDLQSALRSSLDRLVRLMGLT
                     TGWIFLLDPDAWDGPAQLIHTADFQLPLGLAASDAALMKSHGCACQDLFDKGKFKKSV
                     NILECSRIEDAIEAGGDVGGLRIHASVPIRARDRVIGILNVASPSVHQFSDEDLQLMT
                     AVGNQIGLAAERARLYDLTRARRVIEQDALLKLSNALIASRDMQAVVDQVVQVVAEVL
                     SVDGCALVLAVEDEEGGIEFRASVGWDLSMLMLSEGRLKFGWQSPAGTAYYTNQPIRS
                     DDLPTDPRFPDADLYQHLSFRSVLAVPVARSGQAIGALVVNTRAPRHFGDDDVRLLQL
                     MANQAAIAIETARLQERALAEQRLRKELELARQIQASFLPQELPRLSGWEFGAYYASA
                     REVGGDFYDFIPLRDPPSRPTNLPGAVPEVGSRLGMVIADVSDKGVPAALLMAVSRTL
                     VRAVTVSGRSPGDAIQRVNELLLSESQTGQFITLFYAVLDPESAALRFVNAGHNPPLM
                     VCGEAVFSLCAPGMALGIMRSIHLTENEVRFAPGDVLVMYTDGITEALDAGIDEFGVE
                     RLTQVVLANRARSAQDIVNAIVDAVHAFTGGREAFDDISIVALKRAS"
     gene            4433..5218
                     /locus_tag="AC162G_07075"
     CDS             4433..5218
                     /locus_tag="AC162G_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075608.1"
                     /translation="MPDNPSSLWSSCAPFATGTGQVERTPDGARLILPTASRARYSDA
                     QLDDYADLSRRRFPHRPPLRLSLRARFSHPAGALKGTAGFGFWNAPHFDRNRVPSLFP
                     QAIWFFFDSPPSNIALAFGVPGYGWKAACIDAGRPSAWPWAPIAVPVMLACRSPKLYR
                     AIWPRAQRALAISERLLGWQEEPMTGWHSYQLEWLRDGAHWQVDGWTVLETDRPPRGP
                     LGFVAWIDNRCARVTPQGSIGFGLLDTPAQWMEIEEVELIRGA"
     gene            complement(5272..5979)
                     /locus_tag="AC162G_07080"
     CDS             complement(5272..5979)
                     /locus_tag="AC162G_07080"
                     /inference="COORDINATES: protein motif:HMM:NF052375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR5075609.1"
                     /translation="MTCEEIEELLPAYALGALSAEEAATVARHLARCFGHAGSLDAYE
                     AIGDGLAANVQMVSPSPQLRTRLLRSVAAPPSTPPQSRRRAYVGWAVAAALAVLVIAF
                     GAWGLSLQRQIDNQAASRARFLELARRPDAHVVPLEAAEGSVAKGLLIYTDSEAAVWA
                     VALPSLEGAEVFQCWWIDKDGQRVSGGSFVAGEGPGIWFIPMPGNAEDFDGIGITLEP
                     DGDSADPRGPRILSGDL"
     gene            complement(6065..6640)
                     /locus_tag="AC162G_07085"
     CDS             complement(6065..6640)
                     /locus_tag="AC162G_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5075610.1"
                     /translation="MEYAQLDDAMLVTLVARRDEAALAALYDRYSRLVYSVAMRIVGQ
                     RQLAEDITLDSFQRVWQAAGTFRRERGRFVTWLISVTRHRAIDELRRLGVRPEGASVE
                     LNQGIDTGSSREDSVEDVVWLAYKREAVRRALAGLPQPQREALELAYFCGLTQQEIAE
                     RLTTPLGTVKTRMRMGMQKLRRALEEADAVD"
     gene            6695..7483
                     /locus_tag="AC162G_07090"
     CDS             6695..7483
                     /locus_tag="AC162G_07090"
                     /inference="COORDINATES: protein motif:HMM:NF015690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HIR5075611.1"
                     /translation="MQQVQSPTVTTQRSIVCIVPDLFFGVKIENLARQHGLAVVAPSD
                     EQAFMTALAGASLVLIDAGAADRPWAEWVSAAKADPATRDTPIIAFGSHVDRALRERA
                     LEAGVDRYMARSNFVEALPEFMARAAREVTDDACGEPLPDGARRGIHEFNEGKYFEQH
                     ETLERVWRAETRPVRNLYQGILQIGVALLQIERGNAPGAIKTFERAFRWLEPFRPSCQ
                     GVDVERLLRESHAVYEQVKRLGPERIGETPRSSYPHVHLLDKPM"
     gene            7557..8318
                     /gene="fabL"
                     /locus_tag="AC162G_07095"
     CDS             7557..8318
                     /gene="fabL"
                     /locus_tag="AC162G_07095"
                     /EC_number="1.3.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabL"
                     /protein_id="HIR5075612.1"
                     /translation="MDFTNKIAVITGSGRGIGREIALRFARGGADVVVNFFRNRAPAE
                     DTAAEIEKLGRKAAVVKADVGVPDGIDRLIDEAQKAFGGLDILICNAASGYNRPVMEQ
                     RIKGWDWTLNINARSALFLSQKAAPLMEERGGGHIVSISSLGSIRVLPDYVVVGASKA
                     AIETVTRYLAVELAPKNIVVNCVSAGIVETDALKHFRSTKDSHVLVRAAERTPAGRIV
                     TPQDVANVVAFLCTPAAEMIRGQTIVVDGGYTLLV"
     gene            8373..10073
                     /locus_tag="AC162G_07100"
     CDS             8373..10073
                     /locus_tag="AC162G_07100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5075613.1"
                     /translation="MTRQPSSLPPPPSSLRWWGWGRLDRSYELDRRPNFGPFLRKRLG
                     VSGEVTCPPVKLDALSLSEPRLSQRDLSDLRRIFGDDGVCVDRLSRLLHAYGKSYRDL
                     IRIRRGDVGHPPDAVVYPDNEAQIGELFTWAKRSRARLIPFGGGSSVTGGVEPAEKPT
                     DSPTITLDLARLNRVVSIDRASHTATMQGGILGLHLEAALNAEGFTLGHFPQSFEVST
                     LGGWIATRSAGQSSAGYGKIEDMVERVRAITPRGCIEVKPLPASATGPDVLQMLIGSE
                     GAFGVIVEATVRIRPLPGARDYRGVLFHSFEDGAEAIRELMQRGVEVAMARLSDSRET
                     EVSIALARAPATRGARLGMHAGLWLIHERGYDFDSSCLMVLGIEGEQARVKRIKKAAL
                     DVCKRHGGMNVGRSVGRAWLRERFALPYLRDDLLDRGIMIDTVETATTWSSLLGVYHA
                     LNDALEQAIAATGAAPLVMTHVSHSYRDGASLYSTFMGAQRGDPIEQWWSIKRAATEA
                     ILAHGGALSHHHGVGRDHAAWLVRQHGALGVEAFRSLKATFDPDVLLNPGVLGLEIGD
                     "
     gene            10161..10985
                     /locus_tag="AC162G_07105"
     CDS             10161..10985
                     /locus_tag="AC162G_07105"
                     /inference="COORDINATES: protein motif:HMM:NF021214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HIR5075614.1"
                     /translation="MSLDPATDINVWRFDASILLGMLALVGAYSYGVIHLRRRGLWGE
                     EIAGRHVFFFALGVLILFLALASPIDHIGEKYLLSIHMVQHILIAMVAPPLILLGMPR
                     WMMQWALDFFRVGSLVKFVTHPVLAYVAFNVTLIAWHLPALYEAALRNPVVHIVEHAC
                     FIGTGFLSWYPVIDPAGQHARFHPLAKVLYLFLFVIPSGVLGAAFSFAPQPIYATYAE
                     APRLWGATVMGDQAVAGGIMWVPGWAVYFVALSIVFAVWMQHEDRAGAEQADSSSR"
     gene            11071..12735
                     /locus_tag="AC162G_07110"
     CDS             11071..12735
                     /locus_tag="AC162G_07110"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HIR5075615.1"
                     /translation="MPIAIPPSTKDTSASRPFSSATRAENLEWLAREHFDILIIGGGI
                     TGCAAARDAAMRGYRIALVEKEDFASGTSSRSSRLIHGGLRYLEHRQFGLVRESVVER
                     ARLLRNASRIVKPLSFVYPVYHRRYPSYPVLSIGLWLYDLMSIGGRRALPHHRMLHAD
                     HLAEVEPLVATPHIAGAGYYYDATTDDARLTLLTAKAAHHAGAIVANYAQAVGLLKAG
                     TKIAGAHVRAVPAGHELEVKARVVVNAAGPWVDQVLGLDAPTERPVLRPTKGAHLVVP
                     RARLPIRNAIAVRSPRDRRAMFLVPWGDFAIVGTTDTDYDGRPEDAVADRDDCAYLLE
                     SVRGLAPEARLDEADVISTYAGVRPLIAADGRRPSSVSREHAIVESKSGLITIAGGKL
                     TTHRAMAEQLVDRVQDKLLRDSGIAPKLACPTANAPLAASGDIALPDLPGKTAAYLVE
                     AYGADVIEVVRIAGSTAGWADPIVPGLPYLRAEAIYAAEHEMAVTLCDFLMRRTHILH
                     EAQDGALLQAGALAADMGAILGWDDERMQREVAEYEKQVMLARAFH"
     gene            complement(12786..13085)
                     /locus_tag="AC162G_07115"
     CDS             complement(12786..13085)
                     /locus_tag="AC162G_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075616.1"
                     /translation="MASTSAPSAVDMAGVKQRRNKKVSVVVRPARPAWIVDVKEIPES
                     STILFDLVFRPNPNGGWYRRRYEYDGESDVLYHRGEQAFPESEVRSLPDEAEFKG"
     gene            13334..13858
                     /gene="rplI"
                     /locus_tag="AC162G_07120"
     CDS             13334..13858
                     /gene="rplI"
                     /locus_tag="AC162G_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR5075617.1"
                     /translation="MKVLLTRDVDKLGKAGAVKTVANGYARNYLIPQGLAVYASPGAL
                     KQAESIRKMEQKRQTQLANEAATVADRLNGVTLKFQARASETGKLYGSITTKQVVEAL
                     EKEIGAAIDKRKLDMREPIRSLGLHTIHVHLATDLNPSFDVLVERETLAGPEAGSAIE
                     PEMEEQTAEAEAEA"
     gene            13867..14145
                     /locus_tag="AC162G_07125"
     CDS             13867..14145
                     /locus_tag="AC162G_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075618.1"
                     /translation="MNALETAAVRRLIKTVRCPQCGHAFDADDVAVLDHRPDEWLLDV
                     VCHACASHGLVLAEIQKAARAPIDRAEVEEWRRFLHHFRGDMRDLLGE"
     gene            14218..15081
                     /locus_tag="AC162G_07130"
     CDS             14218..15081
                     /locus_tag="AC162G_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024855.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5075619.1"
                     /translation="MSDVGRQLRKAREEKELSLEQVQKATRIKRVYLEAIEADRLDDL
                     PGPVQARGFIRSYASHVGLDPDAVLGRLDGVSSQAIAAPPPSPHLTSKPAAGSTRPVV
                     APRPIAPPPRLSPAPRSSTESSLPLPLPILIAGAIVLFVIGGLLLIQAFRAEAPAPAP
                     TLPASVPLSVSQTVEATPTAPPIAGVSVTLTPSEHVWVRVTHDGLTAFEGFMEPGQSQ
                     TWEAGQQVIVETGNGAALSATVNGEDVGVLGPRNRVVVRAWGVEGEVTPAPTATPAPP
                     PIAEPTATPAP"
     gene            15145..16515
                     /gene="rimO"
                     /locus_tag="AC162G_07135"
     CDS             15145..16515
                     /gene="rimO"
                     /locus_tag="AC162G_07135"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR5075620.1"
                     /translation="MKSNTFYLLSLGCSKNTVDSESMAQLMNRSGYRAASEPGDASIL
                     IVNTCGFIGPARDESIRALRELAESKSKQQLLVAAGCLSQIWGPRLAQEVPGLDGVIG
                     TRRWMDIVEFVDRLRGRQHPEPLYHLPEAARVVGTDEHGVLRAAVAGASAYVKIADGC
                     RRPCAFCSIPLIKGTAVSRPPESIVAEARMLARRGVREAILIAQDLTDYGHDLGIEDG
                     LPVLLEAMCDAAPELKWIRLMYAYPGAVSDRLIEVMATRPQVCHYLDIPLQHGHPAAL
                     RRMRRPANVDWVYRTLGKLRAAMPDIAIRTTFIVGYPGETEAEFEALLKFVEDLQFDR
                     VGGFKYSFEPGTESARLEGHLPEEVKEERYEQLMETQQRISLARNQAQVGRTLDVLVE
                     GHGDGLTLGRTYRDAPEIDGMVILEGEAPLGEFVPVKISGAMTYDLAGTVAIQDAAVV
                     GVEQVR"
     gene            complement(16519..16758)
                     /locus_tag="AC162G_07140"
     CDS             complement(16519..16758)
                     /locus_tag="AC162G_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075621.1"
                     /translation="MSVASRIVTAGGTPSPSSPSLLIDEPNAEEGDMELVLVLMLGFL
                     LLCARSNGQKRADVGVVEVVLAAAVLMLLLGQVAA"
BASE COUNT         2949 a         5625 c         5439 g         2972 t
ORIGIN      
        1 ggcggggaca gtgaaaacga ccgtgtaggt gaaaacatcg ccgaacgcga cggtagacgc
       61 cgacgccgat tttgatattg aaggagaggc gctctggtcc tggcgcgtca cgggcgcccg
      121 ggcgcgcgcc agccggagcg gggcaacggg cagcatcaac gtgcgcccgc caggggcgtg
      181 tagcccatcc ggcagacgcg ctggacgtgt ggtcgctgcc aggccatact cagtgatgtc
      241 ttcaccgccg ggcagaacaa gggtgtccgt tcgcgggggc gcgtgcgggt tcgtcgcgtg
      301 ggatggcaag cgcgtcgaag gctgattcga ggcaaacgat tcggcgcgcg tcgtctgcac
      361 gctgatcgcg caagccagga tcagggcgag cgcgcgcgcc agatgtccgc gccagtcaga
      421 taagcgattg acgttcatgt gtccacctgt cggaatgttg tgatagcctt ccgcggaagg
      481 catctcctcc cccgtaccgt gctgaggaga cgcccggacg cgaccgccag gtggcaatcg
      541 acagctctat tctctactcg ctcggataga ggcctcctta atcgatcttt cgggagaacg
      601 gttatggact accggcgcac gtctgaagga tcttgccggc gtcgctcttg aacggctccg
      661 ccggattcaa ctgcccgaat ttctgaagga cgggcacggc gtccggacag cgccccatct
      721 cggcgtacgt cttgcccagc gcgaacagcg cctccggcga gtcaggcatc agctcgacga
      781 cgcgctgcag cgtctcaacc gcgcgatcgt attctttgtc cagcaagtaa acgttcccta
      841 atccgataag cgcctgcggg aaatccggct ccatctccag cgcgcgctcg aattcccggc
      901 gcgccgcgtc gcgcttgtcc atattgagat aggctgcgcc taactgatag cgcgtatctg
      961 tgtcgccggg gtcgatcgcc agcgcctgct ggaaaagcga cacagcccgc tccagatctc
     1021 cagtctgata ggcgacaacg cccagattgg tcagcgccgg cgcatactcg ggatccagtt
     1081 ccaggaccgc ctcgtagtcc gcctgcgcgg cggcgtagtg gccgatcgcg gtggcgcgat
     1141 cgcccgccag ttcggcctcc aatcccagat tgaagcggct attccccagg gcgaagcgcg
     1201 cttcggtcaa gcccggattg agcgccagcg ctgcttcgag gtagggtatc gcctgctgcc
     1261 attgaccggc cgaggctgca tcctgtccgg cctgcagcgc cgcctcgact gaatcctcga
     1321 tcttcgggtg cgccgtgcgc gcaccgggat cgatcttcac tgctgctgcg taggccgtcg
     1381 ccgcgtcgag caggcgcccg gcatccacgg acaggtcgcc gagcaggtac tgcgccgccg
     1441 cattattcgg gtcctgtcgc agcccggttt gcagccgctc gcgcgcttcc tggctgcgac
     1501 cggccgtgta ataagcctgt cccagcaaga cctgcgcttc tgcgcgcatc ggatcgaggc
     1561 cgacggccgt ctctagctcg gcaatggcct tggccgtgtc gcctgcatcc agcgccgccc
     1621 tcccggcagc caggtgctcg tcgaacgcag gcgtcggcgc ggcgcacgcc gcgagggccg
     1681 ccgccaggtc gagtagaaat cggattttca ttcgtccttg ctcttcagct cgagcagcac
     1741 gacttgcgtc tcgtccggca ggccgctccg atcgagcttg agcatttcct gctcttcgac
     1801 cttgtccaac cccatctctt tcaagaactt cgacaccgga tcgagcttga ggtcggaccc
     1861 tctgaacttg tagtgcatgg ggatgacgat gtgcggctcg agcaggctga cgacctcggc
     1921 agcctgcgaa gcgttcagcg actcgccgcc gccgaccggc acgagcagaa cgtccacctc
     1981 gctgagtgac tcctccatca tggcctgtgt cggcacggtg ttgagatcgc ccaaatggca
     2041 aaccgtgagg ccgtccaatt cgaccaggaa gaccgtgttt cggtccgtcg ccgtcgcgcc
     2101 gctcttgctc gacttgccac cggttttgcg ctcgcccaat accaggccgg tgatgaaggc
     2161 gccggacact tcgtactcgc cggggccaga cagcaccttg aagtcacctt ttactccctt
     2221 gacatagtcg tgatcgtccg cagcgtgact cacggtaacg atgtcggcgc gaacgcgtgg
     2281 cagcgtcagg cccaaatcct tgccgtaggg atcggtcacg accgtcgccg cgcggctgcg
     2341 cagcctgaag caactatgtc ccagccaggt aatctccatt ccctttccct cgatccgttc
     2401 tgcgctcgcg tggacacact ttccgctgca agcgccagga ttgcaaacag tgagtggcat
     2461 tataccaaaa cggagatcaa aagccaacag tctaacgcgt gatataatct ctgctgcgcg
     2521 atcgctaacg gtcctgcaag cgtcccgcaa ccctttccaa ccaacgacga accaacctgc
     2581 atgagcaccg atcgccgcat tgccgaattg acgacgctga atgagattgc tgcgacgctc
     2641 aacgagtcgc tcgatctcca gtcggcgctg cgctcctcgc tggaccggct cgtccggctc
     2701 atggggctga cgaccggctg gatcttcctg ctcgatccag acgcctggga tgggccggca
     2761 cagttaatcc acaccgccga cttccagttg ccgcttgggc tggcggcgag cgacgccgcg
     2821 cttatgaagt cgcacggatg cgcgtgccag gacctgttcg ataagggcaa gtttaagaag
     2881 tccgtgaaca tcctggagtg cagccgcatc gaggacgcga tcgaggcggg cggcgacgtg
     2941 ggcggcctgc gcattcacgc cagcgtgccg atccgcgcgc gcgatcgcgt gatcggcatc
     3001 ctcaatgtcg ccagcccgtc ggttcaccag ttcagcgacg aggacctaca gttgatgacg
     3061 gcggtcggca accagatcgg cctggccgcc gagcgcgcgc gcctgtacga tctgacgcgc
     3121 gcgcggcgcg tgatcgagca agatgcgctg ctcaagctct ccaacgcgct gatcgcgtcg
     3181 cgcgatatgc aagcggtcgt ggatcaggtc gtgcaggttg tggctgaggt gctctcggtg
     3241 gatggctgcg cgttggtgct ggccgtcgag gacgaggagg gcggcattga atttcgtgcc
     3301 agcgtgggct gggatctgtc gatgttgatg ttgtcagagg gcaggctgaa gttcggatgg
     3361 caaagcccgg ccggcacggc ctactacacg aaccaaccga ttcgctccga cgatctgccc
     3421 accgatcctc ggttcccgga cgccgatctc tatcagcatc tctcgtttcg ctccgttttg
     3481 gccgtgcccg tcgctcgctc gggtcaggcc atcggcgcgc ttgtcgtcaa tacccgggcg
     3541 ccgcgccact tcggcgatga cgacgtgcgg ctccttcagc tcatggcgaa ccaggcggcc
     3601 atcgccatcg agaccgcccg gctccaagag cgcgcgctcg ccgagcagcg gctgcgaaag
     3661 gagctggagc tggcgcggca gattcaagcc tcgttcctgc cgcaggagct gccccgcctc
     3721 tccggttggg aattcggcgc gtactacgcg tccgcgcgcg aggtcggcgg cgacttctac
     3781 gatttcatcc cgctgcgcga tcccccgtcg agacccacca accttcccgg cgctgtcccg
     3841 gaagtcggaa gccggctggg catggtcatc gccgacgtgt cggacaaggg cgtgccggcg
     3901 gcgctgttga tggcggtgtc acgcacgctc gtgcgcgcgg tgacggtcag cggtcgcagc
     3961 cccggggacg ccatccagcg cgtgaacgag ttgcttctca gcgaaagcca gaccggtcag
     4021 ttcatcacgc tcttctatgc cgtgctcgat cccgaatccg ctgcgctgcg cttcgtcaac
     4081 gccgggcaca acccgccgct catggtgtgc ggcgaagccg tcttctcgct gtgcgcgccc
     4141 ggcatggcgc tgggcatcat gcggtccatt cacctcaccg aaaacgaggt gcgcttcgcg
     4201 ccgggcgacg tgctcgtcat gtacacggat ggcatcaccg aggcgctcga cgccggaata
     4261 gacgagttcg gcgtggaacg gctcacgcag gtggtgctcg cgaaccgcgc ccgatcggcg
     4321 caggacatcg tgaacgcgat cgtcgacgcc gtgcacgcat tcaccggcgg ccgggaggca
     4381 ttcgacgaca tttcgatcgt ggcgctgaag cgcgcaagct agggctttac tcgtgcccga
     4441 caatccgagc agcctctggt cgtcgtgcgc gccgttcgca acggggacgg gacaggtcga
     4501 gcgaacaccc gatggcgcac gactgattct gcccaccgcg tcgagggcgc gctatagtga
     4561 cgcgcagctc gacgactacg ccgacctgtc gcgccgccgt ttcccgcacc gtccgccgct
     4621 gcgcctctcg ctgcgcgccc gtttctcgca ccctgcaggc gcgctgaagg gcacggccgg
     4681 cttcggcttt tggaacgcgc cccacttcga ccggaaccgc gtgccgagtc tgttccccca
     4741 ggccatttgg ttcttcttcg attcaccgcc gtcgaacatt gcgctcgcct tcggcgtccc
     4801 gggctacggc tggaaggcgg cctgcatcga cgccggccgg ccgtcggcat ggccctgggc
     4861 gccgatcgcc gtacccgtga tgctggcctg ccgctcgccg aagctgtatc gcgctatctg
     4921 gccccgcgcc cagcgcgcgc tggcgatcag cgagcgcctc ctcggttggc aggaagaacc
     4981 tatgacgggc tggcactctt accagctaga gtggctgcgc gacggagcgc actggcaggt
     5041 cgatggttgg accgtgctcg aaaccgatcg gcccccgcgc ggcccgcttg gcttcgtcgc
     5101 ctggatcgac aatcgctgcg cgcgggtcac gccccagggg agtatcgggt tcgggctgct
     5161 ggacacgccc gcacaatgga tggagataga ggaagttgaa ctgatccgtg gtgcgtgacg
     5221 agtgacaagt gagaggtgaa gtgcactttg tcggtcgcca ttcgtcgcgc gttacaggtc
     5281 gccgctcaag atgcgcggtc ccctcggatc cgcgctgtcg ccatcgggtt ccagcgtgat
     5341 gccgatccca tcgaagtcct cggcgttgcc cggcatgggg atgaaccaga tgcccggacc
     5401 ctcgcctgcc acgaaggacc ctccgctcac acgctgaccg tctttgtcga tccaccagca
     5461 ctggaagacc tccgcgccct ccagcgaagg cagcgcgacg gcccacaccg ccgcttcgga
     5521 atcggtgtag atcagcaggc ctttagccac gctgccctcg gctgcctcga gcggaaccac
     5581 gtgcgcgtct ggccgccgtg ccagctccag gaaccgcgcc cgtgaggcag cttgattgtc
     5641 gatctgcctc tgcagcgaca agccccacgc gccaaacgca atgaccagca ccgcgagcgc
     5701 ggctgccaca gcccaaccga cgtatgcacg acgccgactc tgggggggcg tcgagggcgg
     5761 cgctgctacg cttctcagca agcgtgttct caactgaggc gacgggctga ccatctgcac
     5821 gttggctgcc agcccgtcgc cgatggcttc gtacgcgtcg agcgaaccgg cgtggccgaa
     5881 gcacctggcc agatgcctcg caaccgtcgc cgcttcttcg gcgctcaatg ctcccagcgc
     5941 gtaggccggc agcagctctt cgatttcctc acacgtcaca gtcgcttctt ccacagttct
     6001 ctttcacccg ctgtaagtcg atcgcgtcac ttattatacg ttgcgcgcgc atcgtcggat
     6061 gtggctaatc gacggcatcc gcttcctcaa gcgcgcggcg cagtttctgc atgcccatcc
     6121 gcatccgcgt tttgaccgtc cccagtggtg tagtcagccg ctccgcgatt tcctgctggg
     6181 tcagtccgca gaagtaagcc aactccagcg cctctcgctg cggctggggc agtccggcca
     6241 gcgcccggcg cacggcttca cgcttatacg cgagccacac aacatcctcg acagagtcct
     6301 cccgcgacga gccggtgtcg atgccctggt tcagctcgac ggaggcgcct tctgggcgaa
     6361 cgcccagccg gcgcagctca tcgatcgcgc ggtggcgcgt cacgctgatc agccacgtga
     6421 cgaagcggcc tcgctcgcgc cggaaagtgc ccgccgcttg ccacaccctc tgaaaggagt
     6481 cgagcgtgat gtcctccgcc agttggcgct gcccgacgat gcgcatcgcg acgctgtaca
     6541 ccaggcggct gtagcggtcg tacagcgcgg caagcgccgc ttcgtcgcgc cgggcgacca
     6601 gcgtgacgag catcgcatcg tcgagctggg catattccat acccggcatt gtacctgtcc
     6661 cataccggcg tgtcaacgcg tttgacggag ggccgtgcag caggtacaat cgccgactgt
     6721 gacgacacag cgctctatcg tctgcatcgt ccccgacctg ttcttcggcg tgaagatcga
     6781 gaatctcgcc cgccagcacg gcctggccgt cgtcgcccca tccgacgagc aggcgttcat
     6841 gacggcgctt gccggagcgt cgctggtgct gatcgacgcc ggcgcggccg atcgaccctg
     6901 ggccgaatgg gtgagcgccg caaaggccga tccggctacg cgcgacaccc cgatcatcgc
     6961 cttcggctca cacgtcgacc gcgcgctgcg cgagcgagcg ctcgaagccg gcgtcgatcg
     7021 ctacatggcg cgctcgaatt tcgtcgaagc gctgccggag ttcatggctc gcgccgcgcg
     7081 ggaagtcacg gacgatgcct gcggcgagcc gctgccggac ggcgcgcggc gcggcatcca
     7141 cgaattcaac gagggaaagt atttcgagca gcacgagacg ctcgagcggg tgtggcgcgc
     7201 cgagacacgc ccggtgcgca acctgtatca agggatactt caaatcggcg tggccctcct
     7261 gcaaatcgag cgcggcaacg cgccgggcgc gatcaagacc ttcgagcgcg cattccgctg
     7321 gctggagccg tttcgcccct catgccaggg agttgatgtc gagagacttc tacgggaatc
     7381 gcatgcggtg tatgaacaag tcaaacgact tgggccggag cgcatcggcg aaacccctcg
     7441 ctcgagttac cctcatgtcc acttgttgga caagcctatg taaccacctg cgccatctga
     7501 agcggactaa agcccgcact ccggtagcca tcggatagac tctaaaggag acgcgcttgg
     7561 atttcacaaa caaaatcgcc gtcatcaccg gctcgggccg gggcatcggc cgcgagatcg
     7621 cgctgcgctt cgcgcgcggc ggggccgacg tggtcgtcaa cttcttccgc aaccgcgcgc
     7681 ccgccgagga caccgccgcc gagatcgaga agctcgggcg caaggccgcc gtcgtcaaag
     7741 ccgatgtcgg cgtgccggac ggcatcgaca ggctgatcga cgaagcacag aaagccttcg
     7801 gcgggctgga catcctgatc tgcaatgctg cgtcaggcta caaccggccg gtgatggagc
     7861 agaggatcaa gggctgggac tggacgctga acatcaatgc gcgctcggcg ctgtttctgt
     7921 cgcaaaaggc agcgccgctg atggaggagc gcggcggcgg tcacatcgtc agtatcagca
     7981 gtctaggctc gattcgcgtg ttgcccgact acgtcgtcgt cggcgcgagc aaggccgcga
     8041 ttgagactgt cacgcgctac ctggccgtcg agctggcgcc caagaacatc gtcgtcaact
     8101 gcgtctcggc cggcatcgtc gagaccgacg cgctcaagca ttttcgctcg acgaaagaca
     8161 gccacgtgct ggtgagggcg gcggagcgca cgccggccgg gcgcatcgtc actccgcaag
     8221 acgtcgccaa cgtcgtcgcc ttcttgtgta ccccggccgc cgaaatgatc cgaggccaga
     8281 ccatcgtggt agacggcggg tacactttgc tggtgtaaag tgacacgtct cacgcatcac
     8341 gtttcactcg tcacgtttta cccattcccg ccatgactcg acaaccttca tccctccccc
     8401 ctcctccctc ctccctccgt tggtggggct ggggcaggct cgatcgatcg tacgagctgg
     8461 atcggcggcc aaacttcggg ccgtttcttc gcaagcggct gggcgtgagc ggcgaagtga
     8521 cttgcccgcc ggtcaaactc gatgccctca gcctgtccga gccgcgcttg tcacagcgag
     8581 acttatccga cctgcggcgc atcttcggcg acgacggcgt gtgcgtcgat cgattgagcc
     8641 gcctcctgca cgcctatgga aaatcgtatc gcgacctgat tcgcatccgg cgcggcgacg
     8701 tggggcatcc gcccgacgcc gttgtctatc cagacaacga agcgcagatc ggggagctgt
     8761 tcacctgggc aaagcgaagc cgcgcgcgcc ttatcccttt cggcggcggc agcagcgtca
     8821 ccggcggcgt cgagcctgct gagaaaccga ccgatagtcc gacgatcact ctcgatctcg
     8881 cacgtctcaa ccgtgtcgtg tcgatcgatc gagcctcgca cacggcgact atgcagggcg
     8941 gcatcctcgg cctccatctc gaagccgcgc tcaacgccga aggattcacg ctcgggcact
     9001 ttccgcagtc gttcgaggtc tcgacgctcg gtggatggat cgcgacgcgc tcggccgggc
     9061 aatcgtctgc cggctacgga aaaatcgagg acatggtcga gcgtgtgcgc gcgatcacgc
     9121 cgcgcgggtg catcgaagtg aagccgctgc cagcgtctgc gaccgggcct gacgtcctgc
     9181 agatgttgat cggctcggaa ggcgcgttcg gcgtgatcgt cgaggcgaca gtgcgcatcc
     9241 ggccgctgcc gggagcgcgc gattatcgcg gcgtgctgtt tcactctttc gaagacggcg
     9301 ccgaggcgat tcgagagctg atgcagcgcg gcgtcgaggt cgccatggcg cgcctatcgg
     9361 acagccgcga gacggaagtt tcgatcgcgt tggcgcgcgc gccagccacg cgtggggcgc
     9421 gcctcggcat gcacgcgggt ctatggctca tccacgagcg cgggtacgac ttcgactcga
     9481 gctgcctgat ggtgctgggc atcgaaggcg agcaagcccg cgtgaagcgc atcaagaagg
     9541 cagcgctcga cgtgtgcaag cgtcacggcg ggatgaacgt ggggcgaagc gtcgggcgcg
     9601 cctggctgcg cgagcgcttc gcgcttccct acttgcgtga cgacctgctc gatcgtggta
     9661 tcatgattga tacggtcgag acggccacga cgtggagcag cctgctcggc gtgtatcacg
     9721 cgctgaatga cgcgctggag caggccatcg ccgcgacggg tgcggcgccg ctcgtgatga
     9781 cccacgtgtc gcattcgtat cgagacggcg cgtcgctgta cagcacgttc atgggcgcgc
     9841 agcgcggcga cccgatcgag caatggtggt cgatcaagcg cgccgcgacg gaagccatcc
     9901 tggcgcacgg cggcgcgctc agccaccacc acggcgttgg ccgcgatcac gccgcatggc
     9961 tggtccgcca acacggcgca ttgggtgtcg aggcattccg ttccttgaaa gcgacattcg
    10021 acccggacgt gctcttgaac cctggggtgt tggggctgga gattggagac tagagattgg
    10081 agattagagg ttggaggtta gaggttggag gttggaggtt gaagtttgag atttggattt
    10141 tgatctttgg agtttggaag ttgtctttag atccagccac cgatatcaac gtttggcgct
    10201 tcgacgccag catcctactg ggaatgctcg cgttggtcgg cgcgtattcg tatggcgtga
    10261 tccacctgcg caggcggggt ctgtggggcg aggagattgc cggccgtcac gtgttcttct
    10321 tcgccctcgg cgtgctgata ttgttcctgg cgctcgcctc acccatcgac cacattgggg
    10381 agaagtatct gttatcgatt cacatggtcc agcacatcct gatcgcgatg gtcgcgccgc
    10441 ctctgattct gcttggaatg ccgcgctgga tgatgcagtg ggccctcgac tttttccgcg
    10501 tcggctcgct ggtcaaattc gtgacacacc cggtgctcgc ctacgtcgcc ttcaacgtga
    10561 cgctgatcgc ctggcatctc ccggcgctgt acgaagccgc actgcgcaat ccggtcgtcc
    10621 acatcgtcga gcacgcatgc ttcatcggca caggcttcct gtcgtggtat ccggtgatcg
    10681 acccggccgg gcagcacgca cgattccacc cgctggcgaa agttctgtat ctgttcctgt
    10741 tcgtcatccc cagcggtgtg ctgggggccg cgttctcgtt cgcgccgcag cccatttacg
    10801 cgacctacgc cgaagcgccg cgcctgtggg gcgcgaccgt catgggcgat caggccgtcg
    10861 ccggcggcat tatgtgggtt ccgggctggg ccgtgtactt cgtcgcactg tcgatcgtat
    10921 tcgcggtgtg gatgcagcac gaggatcgag ccggcgccga acaagctgac tcgtcgtcaa
    10981 ggtgagggcc tacctgaata gggtggagtt tatccagagg cgtagagact cagtggactc
    11041 cgcgccctct gcgattagaa acctgccgcc atgcccatcg ccattcctcc ttcgacgaaa
    11101 gacacctccg cgtcacggcc cttctcctcg gcgacgcgcg ccgagaacct ggaatggctc
    11161 gcccgtgagc atttcgatat tttgatcatc ggcggcggca tcaccggctg cgcagccgcg
    11221 cgggatgcgg cgatgcgcgg ttaccgcatc gcgctcgtcg agaaagagga cttcgcgtcc
    11281 ggcacgtcgt cgcgatcgtc gaggctcatc cacggcgggc tgcgctatct cgagcaccgg
    11341 cagttcgggc tggtacgcga atcggtcgtc gaacgcgcgc ggctgctgcg caacgcgtcg
    11401 cgcatcgtca agccgctttc gttcgtatac cccgtctacc accgccgcta cccgtcgtac
    11461 ccggtgctct caatcgggct atggctgtac gacctgatgt cgatcggggg caggcgcgcg
    11521 ctgcctcacc atcgcatgct gcacgccgac catctcgccg aggtcgagcc gctggtcgcc
    11581 acgccccaca ttgccggcgc gggctattat tacgacgcga cgaccgacga tgcgcgcctg
    11641 acgctgctca cggcgaaggc agctcaccac gccggcgcga tcgtcgccaa ctatgctcag
    11701 gccgtcggtc tgctgaaggc cggcaccaaa atcgccggcg cgcacgtgcg cgccgtcccg
    11761 gcgggccacg agctggaagt gaaagcccgc gttgtcgtca acgcggccgg gccgtgggtc
    11821 gaccaggttc tggggctgga cgcgccgact gagcgccctg tactccgccc gaccaaaggc
    11881 gcacatctgg tcgtgccgcg tgccagactg cccatccgca acgcaattgc ggtgcgctca
    11941 ccgcgcgacc ggcgcgccat gttcctcgtc ccgtggggcg atttcgccat cgtcggcacg
    12001 accgacacgg actacgacgg ccggccggaa gacgccgtcg ccgaccggga cgactgcgcc
    12061 tacctcctgg aaagcgttcg cggattggcg ccggaagcgc ggctcgacga agcggacgtg
    12121 atcagcacgt acgccggagt gcgcccgctc attgccgccg acggaagacg cccctcgtct
    12181 gtgtcgcgcg agcatgcaat cgtcgaatcg aagtcggggc tgatcacgat cgccggcggc
    12241 aagctgacga cgcaccgcgc catggccgag cagctcgtcg atcgcgtcca agacaagcta
    12301 ctgcgcgact cgggcatcgc gccgaaattg gcgtgcccga cagcaaatgc gccgctggcc
    12361 gcgtcgggcg acatcgccct gcccgatctg ccggggaaga cggcggccta tctggtcgaa
    12421 gcgtacggcg cggacgtcat cgaggtcgtg cgcattgcgg gaagcaccgc gggctgggcc
    12481 gacccgatcg tgccgggcct gccctacctt cgcgcggagg cgatctatgc cgccgagcac
    12541 gaaatggccg tgacgctgtg cgactttctc atgcggcgca cacacatctt gcacgaggcg
    12601 caagacggcg cgctgcttca ggccggcgca ctcgccgccg acatgggggc gatactgggt
    12661 tgggatgacg aacggatgca gcgcgaggtt gcggaatatg agaagcaggt gatgctggcg
    12721 cgggcgttcc actaaacaga agacttcgga agtctttttg agacttccga agtcttggcc
    12781 atcaactaac ctttgaactc tgcttcatcc ggcagcgagc gaacttcgga ctcagggaac
    12841 gcctgctcgc cgcgatggta cagcacgtcg ctctcgccgt cgtattcgta gcggcgccga
    12901 taccagccgc cgttgggatt ggggcgaaag accaggtcga acagaatcgt gctggattcg
    12961 ggtatttcct tcacgtcgac gatccacgcc gggcgagccg gtcgcacgac gacggagacc
    13021 ttcttgttcc ggcgctgctt cacaccggcc atgtccacgg ccgatggtgc agaagtggaa
    13081 gccatacact gagcctcgcc tgtacggaga tcgcaccgaa gtatactcca atccgccgat
    13141 tccgccaacg actgacatgt tttgcgtgca tactcgcggg ctgtcgaagg ggcggcctgg
    13201 atcggcacgt aggcgtgtct ctcgtgagtg ctctcagcgt tctcagcgtc tcagtagtgt
    13261 gatttgacga tccggcctga tctgaggtat cataactgcc gcttcgaatc cgtttcaggg
    13321 agtataagac ataatgaagg tgctgctcac gcgtgacgtc gacaagctgg gcaaggccgg
    13381 cgcggtcaag acggtcgcca acggttacgc gcgcaattac ctgatcccgc agggcctggc
    13441 ggtgtatgcc tcgcccggcg cgctcaagca ggccgagtcc attcgcaaga tggaacagaa
    13501 acgccagacg cagcttgcga acgaggcggc cacggtcgcc gatcggctca atggcgtcac
    13561 gctgaagttc caggcgcgcg ccagcgagac cggcaagctg tacggatcaa tcacgaccaa
    13621 gcaggtcgtc gaggcgctcg aaaaagagat cggcgccgcg atcgacaagc gcaagctcga
    13681 catgcgcgag cccatccgca gcctgggtct gcacacgatc cacgtccacc tggcgaccga
    13741 tctgaacccg agcttcgacg tgctggtcga gcgcgagacg ctagccggcc ccgaagcagg
    13801 gagcgccatc gaaccggaaa tggaagagca gaccgccgag gccgaagccg aagcatgagc
    13861 gtgaacgtga acgccttgga aaccgccgcc gtccgccgcc tgatcaaaac cgtgcgctgc
    13921 ccgcagtgcg gccacgcgtt cgacgccgac gacgtggcgg tgctcgatca tcggccggat
    13981 gaatggctgc tcgacgtagt gtgccacgcc tgcgcgtcgc acgggctggt gctcgccgag
    14041 atccaaaaag cggcgcgcgc gccgatcgat cgcgccgagg tcgaggagtg gcggcgcttc
    14101 ctgcaccact tccggggtga catgcgtgac ctgctgggag agtaagctga gccgcgtgag
    14161 aatccggcca tcggcctgcc gcgcccgccg atggctttac cgtgcaaaga cccggctatg
    14221 agcgacgtcg gccgccaact gcgcaaagcg cgcgaagaga aagagctgag cctcgagcag
    14281 gttcagaaag ccacgcgcat caagcgcgtc tacctcgaag ccatcgaggc cgatcgtctc
    14341 gacgatctgc ccgggccggt gcaggcgcgc ggcttcatcc gctcgtacgc cagccacgtt
    14401 ggcctcgacc ccgacgcagt gctgggacgg ctcgacggcg tatcgagcca ggcaatcgct
    14461 gcgccaccgc cgtcgccgca tttgacgagc aagccagcgg ccggctcgac gcgcccggtg
    14521 gtggcgcccc gccctatcgc tccgccgccc cgcctcagcc ccgcgccgcg tagctcgacg
    14581 gaatcatcgc ttcccctgcc cctgccgatt ttaatcgccg gcgcgatcgt cctgttcgtc
    14641 atcggcggcc tgctgctgat ccaggcattc cgcgccgaag cgcccgcgcc ggcgccgacg
    14701 ctgccggcca gcgtcccgtt atcggtcagc cagacagtcg aggcgacgcc gaccgcgccg
    14761 ccgatcgcgg gcgtatcggt tacgctgacg ccgagcgaac acgtgtgggt gcgcgtgacg
    14821 cacgacggtc tcaccgcttt cgagggtttc atggaaccgg gccagtccca gacgtgggaa
    14881 gccgggcagc aggtcatcgt cgagacgggc aacggcgcgg cgctcagcgc gacggtcaac
    14941 ggcgaagacg tcggcgtgct cggaccacgc aaccgcgtcg tcgtgcgcgc gtggggcgtc
    15001 gaaggcgaag tgacgcccgc gccgacggcc actcccgccc cgccccccat agccgaaccg
    15061 acggcgacac ccgctcctta agcggagatt agcgattaga gattggcgat gcgccatcgg
    15121 ccatcagcca tctgctacca gccaatgaag tccaacacgt tttatcttct ttccctcggc
    15181 tgtagcaaga acacagtcga ttccgaaagc atggcgcagt tgatgaatcg atccggctat
    15241 cgggctgcct cagagccggg cgacgcgtcg atcttgatcg tcaacacgtg cggcttcatc
    15301 ggcccggcgc gcgacgaatc gatccgcgcg ctgcgcgagc tggccgaatc caagtcgaag
    15361 cagcagttgc tcgtcgctgc aggctgcctg tcgcaaatct gggggccacg gctcgcgcag
    15421 gaggtcccag gacttgacgg cgtcatcggc acacggcgct ggatggacat cgtcgagttc
    15481 gtcgatcgcc tgcgcgggcg gcagcatccc gagccgctat atcacctgcc cgaagcagcg
    15541 agagtcgtcg ggacggacga gcacggcgtc ctgcgcgccg ccgtcgccgg agcaagcgca
    15601 tacgtcaaga tcgccgacgg ctgccgcagg ccgtgcgcct tctgctcgat cccgctcatc
    15661 aaggggacgg ccgtcagccg gccgccggag tcgatcgtcg ccgaagctcg catgctcgcc
    15721 aggcgcggcg tgcgcgaagc catcttgatc gcgcaggacc tcaccgacta cggccacgac
    15781 ctcggcatcg aggacggtct gccggtgctg ctcgaagcga tgtgcgacgc cgcgcccgaa
    15841 ttgaagtgga ttcgcctgat gtacgcctat cccggcgcgg tgagcgaccg gctgatcgaa
    15901 gtcatggcga cgcggccgca ggtctgccac tatctcgaca tcccgctgca gcacggccac
    15961 ccggcggcgc tgcgccgcat gcgccggccg gcgaacgtcg actgggtgta ccgcacgctc
    16021 ggcaagctgc gcgcggcgat gcccgacatc gccatccgca ccacgttcat cgtcggctat
    16081 ccgggcgaga ccgaagcgga attcgaggcg ctcctgaagt tcgtcgagga tttgcagttc
    16141 gaccgggtcg gcggcttcaa gtactcgttc gagccgggca ccgagtcggc caggctggaa
    16201 ggccacctgc ctgaggaagt gaaagaggag cgatacgagc agttgatgga gacgcagcag
    16261 cgcatctcgc tcgcgcgcaa ccaggcgcag gtcgggcgga cgctcgacgt gctcgtcgag
    16321 gggcacggcg acggactgac gctcgggcgc acctaccgtg acgcgcccga gatcgacggc
    16381 atggtgatcc tggaaggcga agcgccgctc ggcgagttcg tgccggtgaa gatttccggt
    16441 gcgatgacgt acgacctggc cggcacggtc gcaattcagg atgcggccgt cgtcggcgtc
    16501 gagcaagtca ggtaaagctc acgcggctac ctgacccagc aggagcatga gcacggccgc
    16561 ggccagcacc acctcgacca cccccacgtc ggcgcgcttt tgcccgttcg accgtgcaca
    16621 cagcagcagg aatcccagca tcaagaccag caccagctcc atgtcgccct cctccgcgtt
    16681 cggctcgtct attaggagag acggcgaaga cggcgaagga gttcccccgg ctgtgacgat
    16741 ccgggatgcg acggacatcg gcgtcaacgc gatcgctgtg gagaaaggac gtaagaagac
    16801 gtatgactca cagaaaaatc gctatggaac atgataagaa gatcgccctg gtcgcacacg
    16861 acaataagaa acgcgacctg atagagtggg cccaatataa ccgcgatctc ctggcgcacc
    16921 atcaagtgta tgcgaccggc accaccggcg agatcctgga acaggaactg ggcttcacca
    16981 tcacg
//
LOCUS       DBNHXB010000178        18814 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4402463, whole genome shotgun sequence.
ACCESSION   DBNHXB010000178 DBNHXB010000000
VERSION     DBNHXB010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 18814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18814
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4402463"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="AC162G_07145"
     CDS             complement(<1..695)
                     /locus_tag="AC162G_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015336933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR5075622.1"
                     /translation="MIAIKNLVKHFGYVQAVNGVSLTVAQGKVVVIIGPSGSGKSTVL
                     RCINHLESPTGGEIWVDGIKLTHDHKNINAIRAEVGIVFQQFNLFPHLTALGNIMLAQ
                     RVVRKRRREEAARIARQQLARVGIPEKADVYPAQLSGGQQQRVAIARALAMNPKIMLF
                     DEPTSALDPEMIKEVLDVMLALAKEGMTMVVVTHEMGFARNAADEIIFMDQGLIVERG
                     TPDTFFGNPREERT"
     gene            complement(697..1557)
                     /locus_tag="AC162G_07150"
     CDS             complement(697..1557)
                     /locus_tag="AC162G_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR5075623.1"
                     /translation="MAPITSTPPIAIRRQWNLSQVRWWVWLILIAAADVLLFVAIARD
                     ELYSLIFGKVQQGIVLTIFVTLIAYFSAVIIGLIVGLGRVSKNGIIYNLATLYVQIIR
                     GVPILVQIFFVSFVATPAFFGLLKAIGTALSGVLGPDNFLVQTSTQDVSLLARAVVAL
                     TVAYGGYEAETFRAGIESIGTGQMEAARSLGMSYFQAMRYVILPQAVRNVLPPLGNDF
                     VSMLKDSSLVSVLGVRDITQEARLYVAASFRYPETYYMLAFMYLNMTIPLSMAVRWLE
                     GRMGSGRRAA"
     gene            complement(1620..2522)
                     /locus_tag="AC162G_07155"
     CDS             complement(1620..2522)
                     /locus_tag="AC162G_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR5075624.1"
                     /translation="MKTIRIFSLLMVVALLVAACQQAAAPTTPPPAPTQPPAPQTTEP
                     PEPAALPDLGGREVTVAIENAYLPFNYIRLDNGQAEGWDYDALAEICERLNCVPVFQE
                     IAWDNMIAAVAEGQFDMAADGITITEERAKVVDFSDGYISVQQRIMVRLEDERFTSLD
                     DLKASTDTKLGTQKGTTNYEEAIKLVGEARTIAFDTFGDAVQALIAGDVDGVVIDDTA
                     GQGYVGVNADKIKLLEGSLIGQELGFIFPKGSDLVEPFNAALAAMRADGALDALAQKW
                     FGPEFTVTYEEIGPGAYDNPTPTP"
     gene            complement(2661..3617)
                     /locus_tag="AC162G_07160"
     CDS             complement(2661..3617)
                     /locus_tag="AC162G_07160"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HIR5075625.1"
                     /translation="MITLDDIQAAAQRLSGIANHTPVVTSRTFDERVGASVFFKCENF
                     QRGGAFKFRGAYNMISQLSDDQRRRGVVSFSSGNHAQGVALAAKLLAVPAVIVMPRDA
                     PEVKLAATRGYGAEVVLYNRVEQKRDAIAHRLAEERGLALVPPFDHPHIIAGQGTAAL
                     ELLADVPDLDVLIVPIGGGGLISGCSIAAHALRPGMHVIGVEPEAANDSYLSLQKGER
                     VSTPQSHSIADGLLPTAPGEITFPIMQQHLESIALVSDGEIAEAVRFLALRMKLVVEP
                     SGAAPAAALISGRVANVSGKRIGVIVSGGNVDPYKLAGLLNQ"
     gene            complement(3601..5163)
                     /locus_tag="AC162G_07165"
     CDS             complement(3601..5163)
                     /locus_tag="AC162G_07165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR5075626.1"
                     /translation="MSLASIRSNRTKLALAGLAIAGFTMRVWGIRYGLPYLYHPDEPL
                     GAAVALNMLKMGDLNPHFFGYGSLFFYLNALAYIPYYLIGEIAGFFHTPADIPSLQQF
                     VVGVGRTLMPSQIILGRLVSVALGVLCIPVVYWLGSRLSNRQVGLLAAALVALSPALV
                     LHSQFITPNILATLMVLVTLATLLHLTPQSRWPSYVLVGMALGGAVASKYNAAMLGIS
                     YLVACLTLHGWAALRKFNTYLSPLVAALAFLAVTPYAVLDWPTFIKDTEFHLSYYTVT
                     SHPGMEGNTLEFYVRYLLGQHGVFAFMGLLPVTSYIKTRNRNGLILASFALPYVLYVS
                     TLRIRNDRTILIALPVLLIMAADFVHMVWQRMARSELFRFRRPAQAAWIAFVAIWLVY
                     PAWQAVEQNIRSVTPDAREYSRRWIEASVPTGTRIAAEAYSPFIDPERYEVTYFSALI
                     LNSPEWYVEQGYDLLVLSSGAFARYYNMPERYPAEVAKYDALPARFSEIARFDQNGVI
                     VRVLSVGRDHPR"
     gene            5388..6362
                     /locus_tag="AC162G_07170"
     CDS             5388..6362
                     /locus_tag="AC162G_07170"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR5075627.1"
                     /translation="MALTQYAFFKGRIVPIGEAKISVMTHALNYGTGCFEGIRAYWNP
                     EEEQLLVFRMHEHYERLLDSMGILMMERRYTADELGHVTVELLQMEGYRTDCYIRPIA
                     YKTDEIIGVKLHSLHDDIAIWTTPFGRYVENEEGAHVGFSSWQRVSDNNIPPRGKITG
                     AYANTAFIKTEAVLNGFDEALVLNRNGFVSEGSAENVFMVRDGVLITPPVSEDILEGI
                     TRATMIELAREELGLQVIERPISRTELYVADEAFFTGTGVQVAAIVKVDHRPVGSGAM
                     GPIVKAIRSLYFDVVRGRVRKYRRWCTPVYAEVPDPSASLRAGLAAAR"
     gene            6481..7371
                     /locus_tag="AC162G_07175"
     CDS             6481..7371
                     /locus_tag="AC162G_07175"
                     /inference="COORDINATES: protein motif:HMM:NF022338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HIR5075628.1"
                     /translation="MEWQTDKRLGTLFGAAIIFSLLIFDTGVLLALRNRPVDIWTFVG
                     GLAVVATLPAVAATIYRLIGLRRSGYSIDRNHLTIVWEPIRHVVPTRLIDHVVLGEEV
                     SPRIRFRGGRWPGMWVGSGDVPAIGLTLFNATAPLHQQVFIVTDLAAYAISPIDREGF
                     IEAVKARKAMGPTQDVLQETIRPAFFDWPLWGDRLARRLALGAALLCAALFAFVCLQY
                     PELPERVPLHFDTAGVADRFGGPPNVFILPLIGLLALAVNSLIAVPVYLRERVPAYLL
                     WGGALLAQVLVWVAAVTMLA"
     gene            complement(7368..9023)
                     /locus_tag="AC162G_07180"
     CDS             complement(7368..9023)
                     /locus_tag="AC162G_07180"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HIR5075629.1"
                     /translation="MTPSVQPFSPRRRRNRIVRFFARAILSAFVWDVLIRNLGGRRLA
                     RRSAMARYVALARRFRVLAVDMGGVLIKLGQFVSSRADLLPAEITGELAGLQDEVPPA
                     PFEPVRAVIETELGIPLGEAFSLFDRGPIAAASLGQVYHATLRSGQRVVVKVQRPGID
                     TLIAIDLDALRWVFGWLKRFDAIRRRADLDALFDEFQATLYEEIDYLAEGRNAEHFAA
                     MFADWPRIRIPAINWTHTTRRVLTMEDVGAIKILDVHVYQAQGVDRGDVARTVFEFYL
                     EQIFGDGFFHADPHPGNLFVEPRGEGHFTLNVVDFGMVGRITPDLKAHLRDGFIGLAL
                     RDPHRLVQSMQGAGWLLPSADVEEIERAVDKVFAKYWGISMGELQTLDLHEAGQLVAE
                     FRQLLYRMPFQIPANVLFLARALGILSGLATTIDPEFNVFDTAEPFARTMLVKDGEST
                     LEAFVSQVAEIGAAVIRLPTQMDRLIGAALRGEVRVNVAGSDALAREVRALNASLRRL
                     ISTLLFTALLLAGILLQVNGYPQVGGWVIGVAILVLAWSVLRR"
     gene            complement(9020..10150)
                     /locus_tag="AC162G_07185"
     CDS             complement(9020..10150)
                     /locus_tag="AC162G_07185"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:NF013755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HIR5075630.1"
                     /translation="MARLTPPVLVSDQPAFQAMLRRLASEPRVAVDTESNSLYAYQER
                     VCLLQISIPGADFLVDPLASLELSEMRSIFADASIEKVLHACEYDVICLRRDFGFQLI
                     NLFDTMWAARILGWPRVGLADILKERFGASLDKRWQRYNWGKRPLPADALSYARLDTH
                     YLLRLRDMQMKELEKLNRFEEAQEVFADLARSEAAPRDEAGAGNSFWRVKGAYDLEPA
                     GRAVLRELHLYREAEARRLDRPPFKVIGDRTLVALAESRPTRLEHLRQVPGMTEAQIE
                     RHGRSVLHAVARGKRAQPPQPPPRGGLDPAVADRYERLREWRKGVAASRGVDTDVVIG
                     NAALMTLARRNPHDLAALEGIDGLGPWRRRTYGEAILEVLRT"
     gene            complement(10138..11496)
                     /gene="mgtE"
                     /locus_tag="AC162G_07190"
     CDS             complement(10138..11496)
                     /gene="mgtE"
                     /locus_tag="AC162G_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR5075631.1"
                     /translation="MSSLPPGSIDETIEAIRDALDRASIDEAIAMLERLRAPDQAEVF
                     TELEPEDQAVLLPRMDPDDSADILEQLEDEDAAEVAEQLDPDTLSRIVDEMEPDEAAD
                     LLGDLSKERVDQVLALLEDPDEVRPLLIHPDETAGGRMTSEVFALRRRMTVREAIEAL
                     RSLAPGEDVTYYLFVVDRDNILRGVLSLRKLLTADPSATLESIMDPDVISIQAGADQE
                     EAARLISRYSLFALPVVDRTGHLLGMITHDDLVDVIEDEASEDIYRLGGVIDEDRASG
                     PVRFSVKHRLPWLYVNTFTAFIAAWVISQFQSTIERVAILAAFQAIVAGQGGNAGTQS
                     LTVMVRSIALGDVELRDVWRVLLKEFAVGLTVGVAVGAVVAVIAATLQSNPALGLIIG
                     AAMIGNMLVACLAGAAVPVILKRLKIDPALASGVIVTAITDATGFALFLGLATLFIQW
                     LD"
     gene            11782..12426
                     /locus_tag="AC162G_07195"
     CDS             11782..12426
                     /locus_tag="AC162G_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HIR5075632.1"
                     /translation="MAEATASQGQGITRREFLNYVWGAAFTLFLVQFGVGAYVFALPR
                     FQEGQFGADFDLGLLSDILPQADDAPGEYPDAKTWLLNIGPTEAEKGKGAQGLLAIYK
                     VCTHLGCLYKYAPSNTRFECPCHGSKFHLDGTKILEEGPAPRSLDRFVIEIRDAEGNV
                     VTSTPVNDGRVYSDDPSASLSGTPIEIPADTTGLRVIVKTGVRITGGPPQSDLA"
     gene            12484..14103
                     /locus_tag="AC162G_07200"
     CDS             12484..14103
                     /locus_tag="AC162G_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR5075633.1"
                     /translation="MEPFSTLNDMRKQYGVARTVAIKLNETVERITSGMDINAVRGVL
                     RGDPAPKPNPRVKPHTEGAWFHVWPSFYHVEVTKIFPTFRLGLISTVLFLIEIITGLF
                     LMIWYTPSPLVAYSDMWRILSNVPLGLLMRDIHRLAAEFMVISVALHMWRTYLTGSYK
                     KPRQFTYFTGVILLFFTLFLSYTGYLLPWDQLAYWAVTIGASMVDAAPPKEVGEASGL
                     LLRGGLEIGANGLLRFYLLHVFALPLGVILFLFVHYYKVVRHGHSLPPGFDAPGEDAA
                     RKVPPEKRVPFLPDVATSEVLWFGIITAVLVVMSAFFYHAPLENQADPLNTPLHTVAP
                     WYFLWIQGMLKLGDKTLWGVIVPTALLLAHIVLPYVDYNPSRLARNRRFAWSLSFFSV
                     GVIVILSFMGTSAYLVRAAPETEVNFEFMRPERLGEHDIRTVPFEEMIPGTYDTAALP
                     DSLRNEAPELYDKLKGLQDLIVYYQNPALEHRLDQGHAIVTIADWQIDEEGLSLKKIS
                     MTLEWIEEDGVTPVTLNDYVFAHRNAIYTVE"
     gene            14124..15005
                     /locus_tag="AC162G_07205"
     CDS             14124..15005
                     /locus_tag="AC162G_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5075634.1"
                     /translation="MTTRIFIGGLLFVAIFITVGYVLVNEGLLLVQEQDKSGRMQVFA
                     AGSDGRSIENGADMFTQYCASCHGDDGEGVPGKGPQLNPYLFTTRYPELKLANYPNSL
                     RNFVKIAIAGGRPDYSTYWATKGETYAAPMQTWGDQFGGPLRGDQIESLTDYIMGWEA
                     EAGQPAEIVEFTAIGNDVDQELPAGDAARGKQLWDKTTLYASAKTAPCSACHSLEPGQ
                     TIVGPSLAGIGTRGAETVSGQDAYTYIRHSIQLPGDYIVPGFETADGRSLMPEGLGND
                     MSAQDLADLIEFLLTQQ"
     gene            complement(15124..16431)
                     /locus_tag="AC162G_07210"
     CDS             complement(15124..16431)
                     /locus_tag="AC162G_07210"
                     /inference="COORDINATES: protein motif:HMM:NF021201.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wadjet anti-phage system protein JetD
                     domain-containing protein"
                     /protein_id="HIR5075635.1"
                     /translation="MTAPTLNFTPSADVATILHDLLNAYERRYLRPSAHPERPTRSIR
                     YVLAGTSLPGYYSQIDPAPRQIANEQLQRLERLGLVKLDWLPGETGHLLASVTLVPEG
                     ADALFAWLGRTPEAVRRARLMDSLLGERFRFDGWRLTAVQGSIDQIKAGKSPSPFSLA
                     DEAFNRDLLTALAALDQVKEETPYRVFSVRVFNDSKRLEDLLGALCTLARRGHLDWRE
                     WSNDEILRELSLTANPTHIYLHGPWRLVDDAGQSISLGEFDPSVGLPAAQAHHVRRVS
                     VDSGCVVCVENPTAFYELVRHTKGKPTQAPAFSPGPLAAMCLWGNPSPACRHLLRRLV
                     AELPEAAPLYVWADIDYGGLNILAQLREQVSPRFEPYRMDIETLEAHARWARPLSTGD
                     GRNLNRLAGRASLADMRPLLVHMLERGLKLEQEAIILGSSGAE"
     gene            complement(16659..17030)
                     /locus_tag="AC162G_07215"
     CDS             complement(16659..17030)
                     /locus_tag="AC162G_07215"
                     /inference="COORDINATES: protein motif:HMM:NF039192.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5678 domain-containing protein"
                     /protein_id="HIR5075636.1"
                     /translation="MVAQVSMDANLAESLREAAEQRGASVEDVLNEIVQRYLREVRRE
                     KLWDEFEHYKSMHAELKASYFGQHVAIYEGRLVDHDPDASALVRRIRQRFGRAPVLIT
                     QVEDEPIPEYVIRSPRLVRSE"
     gene            complement(17288..>18814)
                     /locus_tag="AC162G_07220"
     CDS             complement(17288..>18814)
                     /locus_tag="AC162G_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024947.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HIR5075637.1"
                     /translation="CFVRRHFTTSHLNPRVYHRWFIGDRAIPRRIEQLEARLGEIAGE
                     LGGLNERLAALRERLALTRDKVRRFVELEHLLPDLERIPPLAAQIETLHAALNALDTR
                     TVESLRAEVERRKAEVESVQASAAELDRQIGGWQNRAQTLASEIIPDLERKIDEATQS
                     ERQLLSAESAEEMQEEIEREYDRRRERQPLDTVLLNATRYEGDYQTAESKSRDRLRES
                     KHAYSLRYDFGYDEEENAARYLAERDKFLASELPQYEARIAEQRALAEQELVENFIHR
                     LREQIEDARQQLDTLNDTLKDLRFGGERFEFITRPEPSLRQVYDMVMDSQSVLGDSLF
                     ESDFRQKHQKGWDLLFERLTKGAEGEAALELRELQDYRNYLQYDIRIHYPDGDRALLS
                     HINAKKSGGETTTPFYVAMAASFAQAYRLNQPRASDTIRLALFDEAFTKMDTARTASA
                     LQFMREAGLQVLLATPPDKSGALLPHVDAVRTVVRKNNHSFVIEIDRAEMLREVESAA
                     "
BASE COUNT         3487 a         5931 c         5977 g         3419 t
ORIGIN      
        1 gtgcgctctt cgcgggggtt gccgaagaag gtgtccggcg tgccgcgttc gacgatgaga
       61 ccctgatcca tgaagatgat ctcatcggcg gcgttgcgcg cgaagcccat ctcgtgggtg
      121 acgacgacca tggtcatgcc ttccttcgcc aatgccagca tcacgtcgag cacttccttg
      181 atcatctcgg ggtcgagcgc cgaggtcggc tcgtcgaaca gcatgatctt ggggttcatc
      241 gccagcgcgc gggcgatcgc cacgcgctgc tgctggccac cggataactg ggcagggtac
      301 acgtccgcct tttcagggat gccgacgcgc gccaactgct gccgcgcgat gcgcgcagcc
      361 tcctcgcgcc tgcgcttgcg cacgacgcgc tgcgcgagca tgatgttccc cagggcagtg
      421 aggtgcggga agaggttgaa ctgctgaaag acgatgccca cttcggcgcg gatagcgttg
      481 atgttcttgt ggtcgtgcgt caacttaatg ccgtcgaccc agatctcgcc gccggtcggc
      541 gactctaggt ggttgatgca gcgcagcacg gtcgatttgc cggagccgct ggggccgatg
      601 atcaccacca ctttgccctg cgccaccgtc aggctgacgc cgtttaccgc ctgcacgtaa
      661 ccgaagtgct tcaccaggtt cttgatggcg atcatgtcac gcggcgcgtc ggccactgcc
      721 catcctccct tccagccagc gcactgccat tgacagcggg atcgtcatgt tcaggtacat
      781 gaacgcaagc atgtaataag tctccgggta gcgaaacgag gcagccacgt acagccgcgc
      841 ttcctgcgtg atgtcgcgca cgcccagcac cgacacgagc gacgagtcct tcagcatcga
      901 gacgaaatcg ttgcccaacg gcggcagcac gttgcggacg gcctgcggca ggatgacgta
      961 gcgcatcgcc tggaagtagc tcatgcccag cgagcgcgcc gcctccatct gtcccgtgcc
     1021 gatcgattcg atgccggcgc gaaacgtctc ggcctcgtag ccgccatagg ccacggtcag
     1081 cgcgacgacg gcgcgggcga gcaggctgac gtcctgcgtg ctggtctgga cgaggaaatt
     1141 gtcgggaccg agcacgcccg acagcgccgt gccgatcgcc ttcagcagcc cgaagaacgc
     1201 cggcgtggcg acgaacgata cgaagaagat ctgcaccagg atcggcacgc cccggatgat
     1261 ctgcacgtac aacgtcgcca ggttgtagat gatcccattc ttcgagacgc gcccgaggcc
     1321 gacgatcagg ccgatgatga ccgccgagaa gtaggcgatc agcgtgacga atatcgtcag
     1381 gacgatgcct tgctgcacct tgccgaagat gagcgagtaa agctcgtcgc gcgcgatcgc
     1441 gacgaacagc agcacgtcgg cggccgcgat caggatcagc cacacccacc agcgcacttg
     1501 cgacaagttc cattgccgcc ggatcgcgat cgggggagtc gaggttatgg gtgccatggc
     1561 atcgtccatt tacaagacct gccaggtctc aaagacctgg caggtctgcg cttgcttctc
     1621 tacggcgtgg gcgtcggatt gtcgtacgcg cccggcccga tttcctcata cgtgaccgtg
     1681 aactccggtc cgaaccattt ctgggcgagg gcgtcgaggg cgccgtcggc acgcatggca
     1741 gccagcgcgg cgttgaacgg ctcgaccagg tcggaaccct tcgggaagat gaagcctagc
     1801 tcctggccga tgagcgagcc ttccagtagc ttgatcttgt cggcgttgac gccgacgtag
     1861 ccctggcccg ccgtgtcgtc gatgaccacg ccgtccacgt cgccggcgat gagcgcctgc
     1921 accgcatcgc cgaacgtgtc gaacgcgatc gtgcgcgctt cgcccaccag cttgatggcc
     1981 tcctcgtagt tcgtcgtgcc cttctgcgtg cccagcttcg tgtccgtgct ggccttgagg
     2041 tcgtcgaggc tcgtgaagcg ctcgtcctcc agccgcacca tgatgcgctg ctgcaccgag
     2101 atgtacccat ccgagaagtc cactaccttc gcgcgctcct cggtgatggt gatgccgtcg
     2161 gcggccatgt cgaattgccc ctcggccacc gccgcgatca tgttgtccca ggcgatctcc
     2221 tggaacaccg gcacgcagtt gaggcgctcg cagatctcgg ccagcgcgtc atagtcccag
     2281 ccctcggctt ggccgttgtc cagccggatg tagttgaacg gcaggtacgc gttctcgatc
     2341 gccaccgtca cctcgcgacc gcccagatcg ggcagggccg caggctccgg cggctcggtc
     2401 gtttgtggag ccggcggctg ggtcggcgcc ggcggcggcg tggtcggtgc ggctgcctgc
     2461 tggcaggctg cgaccagcag cgccacgacc atcaacaagc tgaaaatgcg aatcgtcttc
     2521 atctcttcct cctcctcagt tgtagcacat ggcgcgccgg ggcggccggc gcgtgtgggg
     2581 taatcactat tctaaaccga tccggtcgat tctcaaaatg gcaacggatg taaaacggat
     2641 aaacggatga cccaggtagg ttattggttc aacagcccag ccagcttgta agggtcgacg
     2701 ttgccgcccg acacgatgac gccgatgcgc ttgcccgata cattcgccac gcgaccgctt
     2761 atcagcgcgg cagccggcgc ggctccgctc ggctcgacga cgagcttcat gcgcagcgcc
     2821 aggaagcgca cggcctcggc gatctcgccg tcgctcacaa gcgcgatcga ctcgaggtgc
     2881 tgctgcatga tgggaaaggt gatctcgccc ggcgcggtcg gcagcagccc atcggcgatc
     2941 gagtgcgatt gcggcgtgga gacgcgctct cccttctgaa gcgacagata cgagtcgttt
     3001 gccgcctccg gctcgacgcc gatcacgtgc atccccggcc gcagcgcgtg cgcggcaatg
     3061 ctgcacccgg agatcaaccc accaccacct atcggcacaa tcagtacatc cagatcgggc
     3121 acgtccgcca acaactccag cgcggcggtg ccctgcccgg cgatgatgtg tggatgatcg
     3181 aacggcggca cgagcgccag gccgcgctct tcggccaacc ggtgcgcgat ggcgtcgcgc
     3241 ttctgctcga cgcggttgta gagcacgact tccgcgccgt agccgcgcgt ggcggctagc
     3301 ttcacttcgg gcgcgtcgcg cggcatgacg atcacggccg gcacggcaag tagtttggcg
     3361 gccagcgcca caccttgcgc gtgatttccc gacgaaaacg ataccacgcc gcgcctgcgc
     3421 tgatcgtcgc tcaactggct gatcatgttg taggcgccgc gaaacttgaa cgcgccgccg
     3481 cgctggaagt tctcgcattt aaagaacacg ctcgcgccga cacgttcgtc gaacgtgcgc
     3541 gaggtgacga ccggtgtatg gttggcgatg ccagacaatc gctgcgcggc ggcctggatg
     3601 tcatcgaggg tgatcacggc ccacactcaa cacgcgtacg atgaccccgt tctgatcgaa
     3661 gcgcgcgatt tcggaaaagc gcgcgggcag tgcatcgtac ttggcaacct cggctggata
     3721 ccgctccggc atattgtagt accgggcgaa cgcgccgctg ctgagcacta gcagatcgta
     3781 gccttgttcg acataccact cgggtgaatt cagaatcaac gcgctgaagt aagtcacctc
     3841 atagcgctcc ggatcgatga acggcgagta ggcttcggca gcaatgcgtg tcccggtggg
     3901 aacgcttgct tcgatccacc ggcgcgaata ctctctggca tccggcgtca ccgatctgat
     3961 gttctgctca acggcctgcc aggcagggta cacaagccaa atggccacga acgcgatcca
     4021 ggcagcttgg gccggccggc ggaatcgaaa caactcactc cgcgccatgc gctgccacac
     4081 catgtgcacg aagtcggcgg ccatgatcag gaggacaggc agtgcaatca ggatcgtgcg
     4141 gtcgttacgg atgcgtaacg tcgaaacata gagcacgtag ggtagcgcga acgacgccag
     4201 gatcagcccg ttccgattgc gagtcttgat gtacgaggtg acgggcagca gacccatgaa
     4261 cgcgaaaacg ccgtgttgtc caagcaggta gcgcacgtaa aactcgagcg tgttgccttc
     4321 catgccggga tggcttgtca cagtataata cgacagatga aactcggtgt ctttgataaa
     4381 cgtgggccag tccagcacgg cataaggggt tacagccagg aaggccagcg ccgcgaccaa
     4441 ggggcttagg taggtgttga acttccgtaa ggccgcccag ccgtgaagtg tcaggcacgc
     4501 gactaggtag gagattccca gcatggctgc attgtatttg ctggctacag cgccgcccag
     4561 cgccatgcct accaacacat aagacggcca tcggctctgc ggcgtcaggt gcagcagtgt
     4621 ggcaagcgtg accagcacca tcagcgtcgc aaggatgttg ggcgtgatga actgcgaatg
     4681 aaggacgagc gccggcgaaa gggcgactaa agcagccgct aatagtccaa cctgccggtt
     4741 cgacagacgc gatcccagcc agtaaacaac cggaatacac agtaccccta gcgcgacaga
     4801 taccaaccgc ccgaggatga tctgtgacgg catcagcgtg cggccaacgc ccaccacaaa
     4861 ttgctgcaga gaaggaatgt cggccggagt gtgaaagaat cccgcaattt caccgatcaa
     4921 gtagtaggga atgtaggcca gcgcgttcag atagaagaaa agcgaaccat atccgaagaa
     4981 gtgcggattc agatcgccca tcttaagcat gttcagcgca accgctgcac cgaggggctc
     5041 gtcggggtga tacagatagg gcaatccata gcggataccc cagaccctca tagtaaatcc
     5101 ggcgattgcc agtccggcca atgccagttt cgtccgattg gaacggattg aggccaagct
     5161 cattctctcc ttgcccagcg tgaaccatcc ggcacacgcc atcgtatata ttgacaacgg
     5221 atttcagaca ccgttagatc gaacactctc cgtcgaaaca tgatgattat agcaagactc
     5281 gacaaaaccg gtttcgtacg tccctctaat tgacatacat atgcaacggt ctataattta
     5341 atagagattg cccacctggg cggagcacat tccacagcga gaggaggatt gccttgactc
     5401 aatatgcgtt cttcaagggg aggatcgttc cgattggaga agccaagatc agcgtcatga
     5461 cccacgcgct caactacggc accggctgct tcgaaggcat ccgcgcttac tggaatcccg
     5521 aggaagaaca actgctggtc tttcgcatgc acgagcatta tgagcggctg ctcgactcga
     5581 tgggcatcct gatgatggag cgccgctaca cggccgacga gctggggcac gtcaccgtcg
     5641 agctgctgca gatggaaggg tatcgcaccg attgttacat ccggccgatt gcgtacaaga
     5701 ccgacgagat catcggcgtg aagctgcaca gcttgcacga cgacatcgcg atctggacga
     5761 cgccgttcgg ccgctacgtc gagaacgaag aaggggcgca cgttggcttc tcttcgtggc
     5821 agcgcgtcag cgacaacaac atcccgccgc gcggcaaaat caccggcgca tatgccaaca
     5881 cggcgttcat caagaccgaa gccgtgctca acggcttcga cgaggcgctc gtgctgaatc
     5941 gcaacggctt cgtctcggag ggcagcgccg agaacgtctt catggtgcgc gacggcgtgt
     6001 tgatcacgcc gcccgtgagc gaggatatcc tcgaaggcat tacgcgggcg acgatgatcg
     6061 agctggctcg cgaagagctg gggctgcagg tgatcgaacg ccccatcagc cggacggaac
     6121 tttatgtggc tgatgaggcg ttttttacgg gcaccggcgt tcaggtggca gccatcgtca
     6181 aggtcgatca ccggccggtc ggcagcggcg cgatgggccc gatcgtgaaa gcgattcgca
     6241 gtttgtactt cgacgtcgtg cgcggcaggg tgcgcaagta tcgccgctgg tgcacgccgg
     6301 tctacgccga agtcccggac ccttcggctt cgctcagggc aggcctggcc gccgcgcgct
     6361 gaagcatccc cttcaagcca ccttcacgaa tggagagata cggggtacaa tcgccccgta
     6421 tctttttgcc tcaccccctc ccctggcgaa gccggggtgg gggtaaggtc tggcactccc
     6481 atggaatggc aaacggataa gcggctcggc actttgtttg gcgctgccat catattctcg
     6541 ctgctcattt tcgacaccgg cgtgctcctg gcgctgcgaa atcgcccggt ggatatctgg
     6601 acgttcgtgg gcgggctggc cgtcgtcgcc acgctgcccg ccgtggctgc cacgatctac
     6661 cggctgatcg gtctgcgccg ctccggctat tcgatcgatc gcaaccattt gacgatcgtc
     6721 tgggagccga tccgccacgt cgtgccgacg cgcctgatcg accacgtcgt cctcggcgaa
     6781 gaggtgtcgc cgcgcatccg gttcagaggg ggacgctggc ccggcatgtg ggtcggcagc
     6841 ggcgatgtgc cggcgatcgg gcttacgctg ttcaacgcga ccgcgccgct tcaccagcag
     6901 gtatttatcg tcaccgacct tgccgcctac gccatctccc cgatcgatcg cgaaggcttt
     6961 atcgaggccg tcaaagcgcg caaggcgatg gggccaacgc aggatgtgct tcaggagacg
     7021 attcgcccgg ctttcttcga ttggccgctc tggggcgatc gcctggcgcg ccgcctggca
     7081 ctcggcgcgg cactgctgtg cgccgcgctg tttgccttcg tgtgcctgca gtacccggaa
     7141 ctgccggagc gtgtgccgct gcacttcgac acggccggcg tggccgaccg cttcggcggg
     7201 ccgcccaacg tattcatcct gccgctgatc ggactgctcg cgctcgcggt gaactcccta
     7261 atcgccgtgc cggtgtatct gcgcgagcgc gtgccggcct acctgctgtg gggcggcgcg
     7321 ctgctggcgc aggttttggt gtgggtggca gccgtgacga tgctggccta acgccgcaag
     7381 accgaccacg ccaataccag gatggcaacc ccgatcaccc agccaccaac ctgcggatag
     7441 ccgtttacct ggagcaggat gcccgccaga aggagcgcgg tgaagaggag cgtgctgatc
     7501 agacgccgca gcgacgcgtt cagcgcgcgc acctcgcgcg ccagcgcgtc gctgccggcc
     7561 acgttgacgc gcacctctcc gcgcagggca gcgccgatca ggcgatccat ctgcgtcggc
     7621 agacgaataa ctgccgcgcc gatctcggcc acctgcgaca cgaatgcctc cagcgtagac
     7681 tcgccatcct tgacgagcat cgtcctggcg aacggctccg ctgtgtcgaa cacgttgaac
     7741 tccggatcga tcgtcgtcgc cagcccggat agaatcccca gcgccctcgc caggaacagc
     7801 acgttcgccg ggatctgaaa cggcatccgg tacaaaagct gacggaactc ggctaccaac
     7861 tggcctgctt cgtgcagatc gagggtctgc agctcgccca tgctgatgcc ccagtatttc
     7921 gcgaacacct tgtcgacggc ccgttcgatc tcctctacgt cggcggaggg cagcagccag
     7981 cccgcgccct gcatcgactg cacgagacga tgggggtcgc gcagcgcgag gccgatgaat
     8041 ccatcacgca ggtgcgcctt gaggtcaggc gtgatgcgcc cgaccattcc gaaatccacg
     8101 acattcagcg tgaagtgccc ttcgccgcgc ggctcgacga acaggttgcc cgggtgcgga
     8161 tcggcatgga agaagccgtc tccgaagatc tgctccaggt agaactcgaa cactgtcctg
     8221 gcgacatccc ctcgatcgac gccttgcgct tggtagacat gcacgtcgag gatcttgatc
     8281 gcgccgacgt cctccatcgt caacacccgg cgcgtggtgt gcgtccaatt gatcgcaggg
     8341 atgcggatgc gcggccagtc ggcgaacatg gcggcgaagt gctcggcgtt gcgaccttcg
     8401 gccaggtaat cgatctcctc gtacagcgtc gcctggaatt cgtcgaacag cgcgtcgagg
     8461 tcggcccgtc gccggatggc atcgaatcgc ttcagccagc cgaacaccca acggagcgcg
     8521 tcgaggtcga tggcgatcag tgtgtcaatg cccggccgct gcaccttgac gacgacgcgc
     8581 tgtccggatc gcagtgtcgc gtggtagacc tgccccaacg acgcagcagc aatgggcccc
     8641 ctatcgaaca agctaaacgc ttcgcccagg ggaatgccca attcagtttc gatgacggcg
     8701 cgaaccggct cgaagggcgc ggggggcact tcatcctgca agccggccag ctcgccggtg
     8761 atttcggcgg gcagcaagtc ggcgcggctg ctcacgaact ggccgagctt gatgaggacg
     8821 ccgcccatgt ccacggccag gacgcggaag cggcgtgcca gagcgacgta gcgcgccatc
     8881 gcggagcgcc gcgccagtcg ccgcccgccc aggttgcgga tcaggacgtc ccagacgaaa
     8941 gccgagagga tcgctcgggc gaagaagcga acgatgcggt tacgacggcg tctgggcgag
     9001 aagggttgaa cgctaggcgt cacgttcgga gaacctccag gatcgcctcg ccgtaggtcc
     9061 tgcgccgcca gggccccagg ccgtcgatgc cttcgagcgc cgcgagatcg tgcgggttcc
     9121 gccgcgccag cgtcatgagc gccgcgttac cgatgacgac gtccgtatcc acgccgcgac
     9181 tcgccgcaac gcctttgcgc cactcgcgca ggcgctcgta gcgatcggcg actgcgggat
     9241 caagcccgcc gcgtggcggc ggctgtggcg gctgggcgcg cttcccgcgc gcgacggcgt
     9301 gcagcacgct gcggccgtgc cgctcgatct gcgcctcggt catgcccggc acctggcgca
     9361 ggtgttcgag ccgggtcggg cgcgactcgg cgagtgcgac gagcgtccga tcgccgatga
     9421 ccttaaacgg cggccggtcg aggcggcgag cctcggcctc gcggtacagg tgtagctcac
     9481 gcagcacggc gcggccggcc ggctcgagat cgtacgcgcc tttcacccgc cagaagctat
     9541 tgccggcgcc cgcctcgtcg cgcggcgcag cctcggatcg cgccagatcg gcgaagacct
     9601 cctgcgcctc ctcgaatcga ttcagcttct ccagctcctt catctgcatg tcgcgcaggc
     9661 gcagcagata atgcgtgtcg agccgggcgt acgaaagcgc gtcggccggg agcgggcgct
     9721 tgccccagtt gtagcgctgc cagcgtttgt cgagcgacgc gccaaagcgc tctttcagaa
     9781 tgtcggccag gcccacgcgt ggccagccca ggatgcgcgc cgcccacatc gtgtcaaaca
     9841 ggttaatcaa ctggaaccca aagtcgcggc gcaggcagat cacgtcgtac tcgcaggcgt
     9901 gcagcacctt ctcgatcgac gcatcggcga atatcgagcg catctcactc agttccaacg
     9961 acgccagggg atcgacgagg aaatcggcgc cggggatgga gatctgcagc aggcagaccc
    10021 tctcttgata ggcatacagg ctgttcgatt cggtatcgac ggcgacgcgc ggctcgctcg
    10081 ccaggcggcg cagcatggcc tggaaggccg gttgatcgga aacgagaacg ggcggggtca
    10141 gtcgagccat tggatgaata gggtcgccag gccgaggaag agcgcgaagc cggtcgcgtc
    10201 ggtgatggcc gtgacgatca cgcccgaggc cagcgccgga tcgatcttga gacgcttgag
    10261 aatcaccggc accgccgcgc cagccaggca ggcgacgagc atgttgccta tcatcgccgc
    10321 accgataatc agaccgagcg ccggattgct ctgcagcgtc gccgcaatca cggccacgac
    10381 ggccccgacg gcgacgccga ccgtcagccc tacggcgaat tccttcaaca gcacgcgcca
    10441 gacgtcgcgc agctcgacat cgcccagcgc gatgctgcgc accatgaccg tgagcgattg
    10501 cgtgccggcg ttgccgcctt gcccggcgac gatggcctga aaagcagcca agatcgctac
    10561 gcgctcgatc gtcgactgaa actggctgat gacccacgcc gcgatgaatg ccgtgaaggt
    10621 gttgacgtac agccagggca ggcggtgctt caccgagaag cgcacggggc ctgatgcgcg
    10681 atcctcgtcg atcacgccgc ccaggcggta gatgtcctcg ctggcttcgt cttcgatcac
    10741 gtccaccagg tcgtcgtgcg tgatcatgcc cagcaaatgc ccggtcctgt cgacgacggg
    10801 cagcgcgaac aggctgtagc gcgagatcag gcgggccgcc tcctcttgat cggcgccggc
    10861 ctggatgctg atcacgtccg ggtccatgat ggattcgagc gtcgcactcg gatcggcggt
    10921 gagcagcttg cgcagcgaga ggacaccgcg cagaatgttg tctcgatcga cgacgaacag
    10981 gtagtacgtc acgtcctcgc cgggggccag gctgcgcagc gcctcgatcg cctctcgcac
    11041 agtcatgcgg cgccgcagcg caaaaacctc gctggtcatg cggccaccag ccgtctcgtc
    11101 ggggtggatc agcaagggac ggacttcgtc ggggtcttcg agcagggcta acacctgatc
    11161 gacccgctct ttcgacaggt cgcccaacaa gtcggccgcc tcgtccggct ccatctcgtc
    11221 gacgatgcgg gagagcgtgt cggggtcaag ctgctcggcg acctccgctg catcctcatc
    11281 ttcgagttgc tcgaggatgt cggccgagtc gtccggatcc atgcgcggca gcaagacggc
    11341 ctggtcttca ggctcaagtt cggtgaacac ctcggcctga tcgggcgccc gcagtctttc
    11401 gagcatggcg atcgcttcgt cgatcgacgc ccgatcgagc gcatcgcgga tagcttcaat
    11461 cgtttcgtct atagagccgg gcggcaagga gctcatcgaa cgagtcttct gcctgcaaag
    11521 tcttcgatcc gcagccaagc gccgatcgag cgtccgtatt atgcccggag tgaatgaact
    11581 gtcaaggaaa gcatagcggc tcgtcgagcg cctcgatcta gattgacatg cccaagcctt
    11641 tgtgatataa attgcaacca gggcgaccga gcgggggcgc agcgtgagcg tatcaaaaac
    11701 acggctcatg ctgttttatg aacttccact ccactgattc ctcagctcgc tcacgacatt
    11761 acggataagg agcttcaagc tatggccgaa gcgaccgcct ctcaaggcca ggggatcacg
    11821 cgccgcgaat tcctcaatta cgtgtggggc gccgcgttca cgctgtttct ggtgcagttt
    11881 ggcgtcggcg cctacgtgtt cgcgctgccg cgctttcaag agggccagtt cggagcggac
    11941 ttcgatctgg gcctgttgag cgacattcta ccgcaagccg acgacgcgcc gggcgaatat
    12001 ccagatgcca agacgtggct attgaacatc gggccgaccg aggcggagaa gggcaagggc
    12061 gcgcagggtc tgctggcgat ctacaaggtc tgcacgcacc tgggctgcct gtacaaatat
    12121 gcgccttcga acacgcgctt cgagtgcccg tgccacggat cgaagttcca cctggatggc
    12181 accaagatcc tggaagaagg cccggccccg cgcagtctgg atcgcttcgt gatcgagatc
    12241 agggatgcag agggcaacgt ggtgaccagc acgcccgtga acgatggccg cgtgtacagc
    12301 gacgacccca gcgcgtcatt gtccggcacg ccgatcgaga tcccggcaga tacgaccggc
    12361 ctgcgcgtga ttgtgaagac tggcgtgcgg attacgggtg gcccacccca gtccgacctg
    12421 gcttaattta gaaaccgggt ttctccgaga aacccggttt ctgggactca ggagacactg
    12481 cccatggagc ctttcagcac tctcaacgac atgcggaagc agtacggcgt ggcgcgcacc
    12541 gtcgcgatca agctgaacga gacggtcgag cgcatcacga gcgggatgga catcaacgcg
    12601 gtgcgaggcg tgctgcgcgg cgacccggcg cccaagccga acccgcgcgt caagccgcac
    12661 actgaaggcg cgtggttcca cgtctggccg tcgttctacc acgtggaagt caccaagatc
    12721 ttcccgacgt tccgcctggg cctgatctcg acggtgttgt tcctcatcga aatcatcacc
    12781 ggcctgttcc tcatgatctg gtacacgccg tcgccgctgg tggcctacag cgacatgtgg
    12841 cgcatcctga gcaacgtgcc gctgggtctg ctgatgcgcg acatccaccg gttggccgct
    12901 gaattcatgg tgatctccgt agcgctgcac atgtggcgca cgtatctgac cggctcgtat
    12961 aagaagccgc gccagtttac gtatttcacc ggcgtgatcc tgctgttctt cacgctgttc
    13021 ctgtcgtaca ccggatacct gctgccgtgg gatcaactgg cctactgggc agtgacgatc
    13081 ggcgcgtcga tggtagacgc ggcgccaccc aaggaagtgg gcgaggcgtc cggcctgctg
    13141 ctgcgcggcg gcctggagat cggcgcgaac ggactgctgc gcttttacct gctgcatgtg
    13201 ttcgcgctgc cactcggcgt gatcctgttc ttgttcgtgc actactacaa ggtcgtgcgt
    13261 cacggccatt cgctcccgcc gggattcgac gcgccgggcg aggacgcggc gcgcaaggtg
    13321 ccgccggaaa agcgcgtgcc gttcctgcct gatgtggcga cgagcgaggt gttgtggttc
    13381 ggcatcatca cggcggtgtt ggtcgtgatg tcggcgttct tctaccacgc gccgctggag
    13441 aaccaggctg acccgctcaa cacgccgctg cacacagtcg cgccgtggta cttcctgtgg
    13501 attcaaggca tgctcaagct aggcgacaag acgctgtggg gcgtcatcgt gccgacggcc
    13561 ctgctgctgg cgcatatcgt gctgccgtac gtcgactata acccgagtcg cctggcccgg
    13621 aaccgccgct tcgcctggtc gctgtcgttc ttcagcgttg gcgtgatcgt catcctgtcg
    13681 ttcatgggga cttcggctta cctggttcgg gctgcgcctg agacggaagt gaacttcgag
    13741 ttcatgcggc cggagcggct gggcgagcac gacatccgca cggttccgtt cgaggagatg
    13801 atccctggca cctacgacac ggcggcgctg cccgactctc tcaggaacga agcgccggaa
    13861 ctgtacgaca agctgaaggg cctccaggat ttgatcgtgt attaccagaa cccggcgctc
    13921 gagcatcggt tggaccaggg gcacgccatc gtcacgatcg cggactggca gatcgatgag
    13981 gagggtctgt ccttgaagaa gatctcgatg acgctcgaat ggatcgaaga agacggcgtg
    14041 acgcccgtga cgttgaacga ctacgtcttc gcgcaccgca acgcaatcta cacggtcgaa
    14101 taggccgagt ttgaggcgag gccatgacaa cgcgaatttt catcggcggt ctgctgtttg
    14161 tggcgatctt catcaccgtc ggctacgtcc tggtgaacga gggcctgctg ctggtgcagg
    14221 agcaggataa gtcgggccgg atgcaagtgt tcgcggccgg gtcggacggg cggtcgatcg
    14281 agaacggggc cgatatgttc acgcagtact gcgcgtcgtg ccacggcgac gacggcgaag
    14341 gcgtgcccgg taagggtccg cagttgaatc cgtacctgtt caccacgcgc taccccgagc
    14401 tgaaactggc caactacccg aactcgctca ggaacttcgt caagatcgcc atcgcaggcg
    14461 gccgcccgga ttattccacg tattgggcga cgaagggcga gacgtatgcg gccccgatgc
    14521 agacgtgggg agatcagttc ggcgggccgc tgcgcggcga ccagatcgag agcctgacag
    14581 actacatcat gggttgggaa gcggaagcgg gccaaccggc cgagatcgtg gagttcacgg
    14641 ccatcgggaa cgatgtggat caggagctgc cggcgggtga cgcagcgcgc ggcaagcagc
    14701 tctgggacaa gacgacgttg tacgccagcg ccaagacggc tccctgctcg gcgtgccact
    14761 cgctggagcc gggccagacg atcgttggcc cgtcgctggc gggcattggc acgcgcggcg
    14821 cagaaaccgt ctccggccag gatgcctaca cgtacattcg tcactcgatc caactgcccg
    14881 gcgattacat cgtgccgggc ttcgagacgg cggacggcag atcgcttatg ccggaagggc
    14941 taggcaacga catgagcgcg caggacctgg ccgacctgat cgagttcctg ctgacgcagc
    15001 aatagaatca accgacgagt tgaaggccca tgggcgaccc atgggccttt tcattacgcc
    15061 tttgtgggca actgcttcga cgacacgcga gaggcgctga tcttctggtg ggattgagcg
    15121 agatcattca gcgccgctgc tgcctaagat gatggcctct tgttcgagct tcagaccgcg
    15181 ctcaagcatg tgaacgagga gaggtcgcat atccgcgaga gaagcgcggc cggccaggcg
    15241 attgagattt cgcccatccc ccgtagacag cggacgcgcc cagcgcgcgt gcgcctccag
    15301 cgtctcgatg tccatgcgat acggctcgaa ccgcgggctg acttgctcgc ggagctgcgc
    15361 caggatgttc agcccgccgt agtcgatgtc ggcccagacg tacagcggcg cggcttcggg
    15421 cagctcggcg acgaggcgac gcagcaggtg gcggcacgcg ggcgacgggt tgccccacag
    15481 gcacatcgcg gcaagcggac cgggcgagaa agccggagcc tgtgtgggtt tccccttcgt
    15541 gtgtcgcaca agctcgtaga aggcggttgg attctccaca cacacgacgc atcctgaatc
    15601 gacgctgacc cgccgcacat ggtgcgcttg cgccgccggt agtccgaccg acggatcaaa
    15661 ctcgcccagc gaaatacttt gcccggcgtc gtcgaccaga cgccatgggc cgtgcaggta
    15721 gatgtgcgtg ggattcgcgg tcaggcttag ctcgcgcaga atctcgtcgt tcgaccattc
    15781 tctccagtcg aggtggccgc gccgggcgag cgtacacagc gcgccgagca aatcctccag
    15841 gcgtttgctg tcgttgaaca cgcgcacgct gaagacgcga tacggcgtct cctctttcac
    15901 ctgatcgagc gcggccagcg ccgtcagcag atcgcggttg aaggcctcgt cggccaggct
    15961 gaaaggcgat ggcgacttgc cggccttgat ctgatcgatg ctgccctgaa cggcggtgag
    16021 ccgccagccg tcgaagcgga agcgctcgcc cagcagcgag tccatcaagc gtgcgcggcg
    16081 gaccgcctcc ggcgtgcggc ccaaccacgc gaaaagggca tcggcgcctt cggggacgag
    16141 cgtcaccgaa gcgagcagat ggccggtttc gccgggcagc cagtcgagct tcaccaggcc
    16201 gagccgttcg agtcgttgaa gctgctcgtt cgcgatctgg cgcggcgcag gatcgatctg
    16261 gctgtagtag ccaggaagag acgttcctgc gaggacgtaa cggatagagc gtgtggggcg
    16321 ttccgggtgt gcgcttggcc gcagatagcg acgctcgtaa gcgttgagca ggtcgtgcag
    16381 gatagtggcg acgtcggcgg aaggagtgaa gttcagtgtg ggcgcggtca tgcctgttta
    16441 cgacgcttgc acacgctggc gatgtccaaa atggctgcgg agtgttaccc agccactctc
    16501 gaccgcacca gcctcttcaa gcatttgagt cgggaccaac gttgcgtcgg cgcctgagtc
    16561 gagcaaagcc ggaaaagggc ccaggtgccc gtcatcgctg gttaagagga tcgtcaatgc
    16621 gggaaaaggc ggtcgataga cagtctggta aggtgtgctc attccgatcg caccaggcgt
    16681 gggctacgga tcacatactc cgggatcggt tcatcttcca cctgagtgat caagacgggg
    16741 gcgcggccga atcgctgtcg aatgcgccga acgagcgccg atgcatcggg atcgtgatcg
    16801 accaagcggc cttcgtagat ggctacatgc tgaccgaagt agctcgcttt aagctcagcg
    16861 tgcatcgact tatagtgctc aaactcgtcc cagagtttct cgcggcgaac ctcgcgcaaa
    16921 tatctctgca cgatttcatt aaggacgtcc tcaacactgg ctcctcgctg ttcggcagct
    16981 tcgcgcagcg attccgcaag attcgcatcc attgacacct gcgcgaccat agcatactct
    17041 ccgtcaaatt ggcttcccta acctgttttc cgcagttccc accgcagcct gccctgagct
    17101 gagtcgaagg gttgcactgc ggcggcctgg gacggcagtg cacctgccgt cccaacaagg
    17161 tgcaacttca gtcgcaaagg taacgactga agtcgtcact acgattcgtt tgtctccgct
    17221 tctgtgctca cggatgatcc atccggcggt agcgggtgcg aaatataggc taagagtttt
    17281 ccacgaatca cgccgcgctc tcaacttccc tgagcatctc ggccctgtcg atctcgatca
    17341 cgaacgagtg gttgttcttg cgcacgacgg tgcgcacggc atcgacgtgg ggcaagagcg
    17401 cgccggactt gtcgggtggc gtggcgagca atacttgcag gccggcctcg cgcatgaact
    17461 gcagcgcgct ggccgtgcgc gcggtgtcca tcttggtgaa cgcttcgtcg aacaaggcca
    17521 ggcggatagt gtccgaggcg cgcggctggt tgaggcgata cgcctgcgcg aacgaggcgg
    17581 ccatcgccac gtagaacggc gtcgtcgtct cgccgcccga ttttttagcg ttgatgtggc
    17641 tgagcagggc gcggtcgccg tctggataat gaatgcggat gtcgtattgc agatagtttc
    17701 gatagtcctg cagctcgcgc agctcgagcg cggcctcgcc ttccgcgcct ttcgtcaggc
    17761 gctcgaacag caagtcccag cctttctggt gcttctgccg gaagtccgac tcgaacagcg
    17821 agtcgcctag aaccgattgg ctgtccatga ccatgtcata cacctgccgc agcgacggct
    17881 ccgggcgggt gatgaactcg aagcgctcgc cgccgaagcg caggtctttc agcgtgtcgt
    17941 tcagggtgtc caactgctgg cgagcgtctt cgatctgctc gcgcagacgg tgaatgaaat
    18001 tctcgaccaa ctcctgctcg gcgagcgcgc gctgctcggc gatgcgcgcc tcgtactgcg
    18061 gcaattcgga cgcgaggaac ttgtcgcgct cggccaggta acgcgcggca ttttcctcct
    18121 cgtcgtagcc gaaatcgtaa cgcaggctat aggcatgctt gctttcccgc agcctgtcgc
    18181 ggcttttgct ctcggccgtc tgataatcgc cctcgtagcg cgtcgcgttg agcagcaccg
    18241 tgtcgagcgg ctggcgctcg cgccggcggt cgtactctct ctcaatctcc tcttgcatct
    18301 cttcggcgga ctcggccgac aggagctgtc gctcactttg tgtcgcctcg tcgatcttcc
    18361 gctccagatc ggggatgatt tcgcttgcca gagtctgcgc ccggttctgc cagccgccta
    18421 tctgccggtc cagctctgcg gcgctcgcct gcaccgattc gacttcggcc ttccggcgct
    18481 cgacctcggc gcgcagcgat tcgacggtac gtgtgtcgag cgcgttgagc gcggcgtgca
    18541 gcgtctcgat ctgcgccgcc agcggcggga tgcgctcgag gtcaggcagc aggtgctcca
    18601 gctcgacaaa gcggcgcacc ttgtcacgcg tgagcgcgag ccgctcgcgc agcgcagcca
    18661 gccgctcgtt tagcccgccc agctcgccgg cgatctcgcc gagccgcgct tccagttgct
    18721 cgatgcggcg cgggatggcg cgatccccga tgaaccagcg gtggtacacg cgcggattga
    18781 ggtggctggt cgtgaaatgg cggcgcacga agca
//
LOCUS       DBNHXB010000179         4109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4570284, whole genome shotgun sequence.
ACCESSION   DBNHXB010000179 DBNHXB010000000
VERSION     DBNHXB010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4570284"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="AC162G_07225"
     CDS             complement(<1..1043)
                     /locus_tag="AC162G_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075638.1"
                     /translation="MPETLSFVMFARGRRPGPPWLRAMDDILLAAARDTLERAAGAFD
                     RIILATPDAEWAAPLDDPEIEVDLDPPHVPFHFGRRLADLVARFDLERIVYTGTASAP
                     LLTRDDLAAIARAAREADATVIANNIYSTDWAAIVPAQAVTGLPERLHTDSALGWVLS
                     KEAGLPPRLWPRSPASLLDIDTPLDALIAAQHPSAGPRLRAAVAASGWSSERIDAARR
                     VMATPAMWLTLIGRVPSWSTSLLEQKTQCWVRVFSEERGMRAAGRLASGGVRSLVARH
                     YASVGPHRFFADLAEMTDAVLFDSRVLLAAHNIWPDDAARFASDLLMPEVIDDPFLRE
                     FTLAAAGCPIPILL"
     gene            1186..1482
                     /locus_tag="AC162G_07230"
     CDS             1186..1482
                     /locus_tag="AC162G_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HIR5075639.1"
                     /translation="MSTSMHRLQISLPRSQARFLAERARRDGVSIAEVIRQLIQREEE
                     AAAAQQSSVDSIWEIVGMIADDQPLIDGIPVSEQPDLYLAEAALPRRTTKKARR"
     gene            1486..1896
                     /locus_tag="AC162G_07235"
     CDS             1486..1896
                     /locus_tag="AC162G_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR5075640.1"
                     /translation="MRRALVDTGAIFAMVVKPDAHHRTASAFVKKWLAEKSVFVLLDL
                     VFVETMTLMKRRYGAQAALRVGRELRQNPVYLWTAFTPELERETWAVFQKYDDKEWSY
                     TDCALLVLSQQLRIPEVFAFDEHFKQMPGITRLP"
     gene            2031..2702
                     /locus_tag="AC162G_07240"
     CDS             2031..2702
                     /locus_tag="AC162G_07240"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5075641.1"
                     /translation="MPRHFPRPSVTVDVVIFTLRDDDLQVLLVKRKAAPYTGRWAIPG
                     GFMRIDEPLEEAARRELSEETGVRDVYMEQLYTFGDPKRDPRGRVITVAYFALVPAPL
                     AVRAGGDASHACWWSMYGLPPLAFDHADILDYALTRLRYKLEYTAVGFQLLPDTFTLS
                     ELQKAYEVVLGTALDKRNFRRKILEAQVIEETGEFRTGEGRPAMLYRYRSDAVAEVRA
                     RRLFP"
     gene            2912..3661
                     /locus_tag="AC162G_07245"
     CDS             2912..3661
                     /locus_tag="AC162G_07245"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR5075642.1"
                     /translation="MNTQDFIRHSTPFLTWLNEWHAALPQPNLESVVSDPARTAILSI
                     DVINAFCYTGPLASRRVQRIVHPIVRLMTAAYAAGVRHFISIQEDHDPQAIEFEAYPP
                     HGIHGTEDAEAVPEYKALPFWNEFSVMRKNSISATLGTTLDAWLDAHPEVSTFIVVGD
                     CTDLCTYQLAMHLRLRANAMQKRGVRVIVPEDCTDTFDTPIEVAKQIGAMPHDAELLH
                     RIFLYSMAENRVEVVKRIAVPETEPAAMAAA"
     gene            3680..3883
                     /locus_tag="AC162G_07250"
     CDS             3680..3883
                     /locus_tag="AC162G_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075643.1"
                     /translation="MSIELAILIALLLYIAHLRIKLWLDGKVIQSFQKSAIIVPKAEK
                     KQDLGAIALIIAALALLLALAAN"
     gene            3885..>4109
                     /locus_tag="AC162G_07255"
     CDS             3885..>4109
                     /locus_tag="AC162G_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR5075644.1"
                     /translation="MSLFNQTRLTNAVFKLDVERMRRGWYSDKNFENIEATLRKLAAE
                     HAAYDGRYPSVHGVELAGADVGNMEVEMQWF"
BASE COUNT          791 a         1305 c         1286 g          727 t
ORIGIN      
        1 agcagaatcg ggatcgggca cccggcggct gccagcgtga actctcgtaa gaacggatcg
       61 tcgatcactt cgggcatgag caggtcagac gcaaaccgcg cggcatcgtc cggccagatg
      121 ttgtgcgcgg ccagcagcac gcgcgaatcg aacagaaccg cgtccgtcat ctcggccagg
      181 tcggcgaaga agcgatgcgg accaaccgat gcatagtgtc tggcgacgag tgagcgcacg
      241 ccgccgctcg ccagtcttcc cgccgcgcgc atgccgcgct cctcgctgaa cacgcgcacc
      301 cagcactgtg tcttttgctc caacaggctc gtcgaccacg acggcacgcg cccgatcagc
      361 gtcagccaca tcgccggtgt cgccatgact cgccgcgctg catcgattcg ctcgctcgac
      421 cagcccgatg ccgcgacggc cgcacgcagg cgcggcccgg cgctgggatg ctgcgcggcg
      481 atcagtgcgt cgagcggcgt gtcgatgtcg agcaggctgg ccggcgagcg cggccacagt
      541 cgggggggaa gtcccgcttc ctttgacagc acccagccca gtgcgctgtc ggtatgcagc
      601 cgctcgggca gcccggtcac cgcctgcgcc gggacgatcg ccgcccaatc ggtcgagtag
      661 atgttgttgg cgatgacggt ggcgtcggct tcacgcgccg cgcgtgcgat cgccgccaga
      721 tcgtcgcggg tcaggagcgg cgcactcgcc gtcccggtgt acacgatgcg ttcgagatcg
      781 aagcgcgcga cgagatcggc caggcggcga ccgaagtgga acggcacatg ggggggatcg
      841 agatcgacct caatctccgg atcgtccaac ggcgcggccc attcggcgtc gggcgtggcg
      901 agaatgatcc gatcgaaggc gccggccgcg cgctcgagcg tatctcgcgc cgccgcgagc
      961 aggatgtcgt ccatcgcgcg cagccagggc gggccgggtc ttcgcccgcg cgcgaacatg
     1021 acaaaactca gagtttcggg catggttatc ttttccgggt agacctgtca ggtctgatga
     1081 tgcgatggca agtctaacat cctagaatgc atgcaccaaa cggctcgcct ttgacaaagg
     1141 ggtgatggtg atgtagtatt attacatcac tcacgggagg cggctatgtc gacttcgatg
     1201 caccgtttgc aaatctcact gcctcggtct caagcccgat tcctggccga gcgagcgcgc
     1261 cgagatgggg tgagcattgc cgaggtgatt cggcagctca tacagcgcga ggaagaggcg
     1321 gccgctgcgc agcagtcgag cgtcgactcg atctgggaga tcgtggggat gatcgcggac
     1381 gatcagccgc tgattgacgg catcccggtc agcgagcagc cagaccttta cctggccgaa
     1441 gcggctctcc ctcgtcggac aaccaagaag gcgcggagat agcccatgcg ccgagccttg
     1501 gtcgacactg gcgcaatctt cgcgatggtt gtcaagccag atgcgcatca ccgtacggcc
     1561 agtgccttcg tgaagaagtg gctggcggag aaaagcgtct tcgttctgct agacttggtc
     1621 ttcgtcgaaa cgatgacgtt gatgaagcgc aggtacggag cgcaggcggc actccgggtc
     1681 gggcgcgagc tgcgccagaa tccggtttac ctttggacgg ccttcacccc cgaactggaa
     1741 cgcgagacat gggccgtgtt ccagaagtac gacgacaagg aatggtctta cacggactgc
     1801 gccttgctcg tgctgtccca acagttgagg atcccagaag ttttcgcgtt cgatgagcat
     1861 ttcaagcaga tgccaggcat cacgcggctg ccgtgaagcg ccagcggcgg cggttggatt
     1921 atggctttag gcgagaatgc atcctcgaag agatgtacac gttttgtgct tgacacggct
     1981 ccgctttcgc agtaaattcg tgttaatccg acaccaacta tcgagtcccg atgcccaggc
     2041 actttccccg tcccagtgtc accgtcgacg tcgtcatctt caccctgcgc gatgacgact
     2101 tgcaagtctt gctcgtcaag cgcaaagcgg cgccgtatac cggccgctgg gcgatccccg
     2161 gcggcttcat gcgcatcgac gagccgctcg aagaggccgc ccgccgcgag ctgagcgagg
     2221 aaaccggcgt gcgcgacgta tacatggagc agttgtacac cttcggcgat ccaaagcgcg
     2281 atccgcgcgg gcgcgtcatc accgtggcct acttcgcgct cgtccccgcg ccgctggccg
     2341 tgcgcgccgg cggcgatgcg agccacgcct gctggtggtc gatgtacggt ttgccgccgc
     2401 tcgcgttcga ccacgcggac atcctggact atgctctgac gcgcctccgg tacaagctcg
     2461 aatataccgc cgtcggcttc cagttgctgc ccgacacgtt cacgctgtcc gagctgcaaa
     2521 aggcctacga agtcgtgctc ggcacggcgc tggacaagcg caacttccgg cgaaagatcc
     2581 tggaagcaca agtgatcgag gaaacggggg aatttcgcac cggcgagggc cgcccggcga
     2641 tgctgtatcg gtaccggagc gacgccgtgg ccgaagtccg cgcgcgccgg ctgttcccgt
     2701 agaaaggctt cggaagtctt cgacttccga agtctgaagc aatccgctct tgacaaagcg
     2761 ccgaattgcg atatacttag tgtcagaagt acactatatg ggcgatatag aaagacttcg
     2821 gaagtcttca aggcgcttcg gcctgcggaa gccctgcgca gacttccgaa gtctgaagca
     2881 agtgtcagaa ctgcatgaat caggaggggc catgaacacg caagatttta tccggcacag
     2941 cacgccgttt ttgacctggc tgaacgagtg gcatgcggcg ctgccgcagc ccaatctcga
     3001 atcggtggtg agcgatccgg cccggacggc catcctctcg atcgacgtga ttaacgcctt
     3061 ctgctacacc ggcccgctgg cgagtcgccg tgtgcagcgc atcgttcacc ccatcgtccg
     3121 gctgatgacc gccgcctacg ccgccggcgt gcgacacttc atctccattc aggaagatca
     3181 cgatccgcag gccatagagt tcgaggcgta cccgcctcac ggcattcacg gcaccgaaga
     3241 cgccgaggcc gtgcccgaat ataaggccct gcccttctgg aacgagttca gcgtgatgcg
     3301 taagaatagc atcagcgcca cgctgggcac aacgctcgat gcctggctcg acgcccatcc
     3361 cgaggtgagc accttcatcg tcgtcggcga ctgcaccgat ctgtgcacgt accagttggc
     3421 gatgcacctc cgcctgcgcg ccaacgccat gcagaagcgc ggcgtgcggg tgatcgtgcc
     3481 ggaagattgc accgatacgt tcgacacccc gatcgaggtg gcgaagcaga tcggcgcgat
     3541 gccccacgac gccgagctgc tgcaccgcat cttcctctac agcatggccg agaatcgcgt
     3601 cgaggtggtc aagcgcatcg ccgtgccgga gaccgagccg gcggcgatgg ccgccgcctg
     3661 agcgcctgcc tgaaaagtca tgagcatcga actggccatt ctgatcgcgc tgctgctgta
     3721 catcgctcac ctgcgcatca agctctggct cgacggcaag gtcatccagt ctttccagaa
     3781 gtcggcgatc atcgtgccga aggccgagaa gaaacaagac ctgggcgcca tcgccctgat
     3841 catcgccgcg ctggccttac tgctcgctct cgcagccaat taggatgagc ctcttcaacc
     3901 agacccgcct gaccaacgcc gtcttcaagc tcgacgtcga gcgcatgcgc cgaggctggt
     3961 acagcgacaa gaatttcgag aacatagaag ccacgctgcg caaactggcc gccgagcatg
     4021 ccgcctatga cggccgctat ccttccgtgc acggcgtcga gttggccggc gcggacgtcg
     4081 ggaacatgga agtcgagatg cagtggttc
//
LOCUS       DBNHXB010000180         5675 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1215410, whole genome shotgun sequence.
ACCESSION   DBNHXB010000180 DBNHXB010000000
VERSION     DBNHXB010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1215410"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="AC162G_07260"
     CDS             complement(<1..596)
                     /locus_tag="AC162G_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR5075645.1"
                     /translation="MSPLLKRLEDAYPDDVRVVYRHFPLTEIHDKAQLSAEASEAAGA
                     QGKFWEMHDVIFEKFDAWTSLGVDQFRTELSNYAEEIGLDAKQFDADLDSGKFTQKVQ
                     AARELAVQIGLSGTPFLLVNEAPWPESLSYLSYNNLEGLVKYFAELPDMQYDAYPETV
                     IDENKTYIATIATDQGDIVVELYAKQAPLAVNSFVFLAR"
     gene            complement(612..1055)
                     /locus_tag="AC162G_07265"
     CDS             complement(612..1055)
                     /locus_tag="AC162G_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR5075646.1"
                     /translation="MKRLVWLAVIGALIVSACAPQSTGTPATDTSSGAGPTAAPPSIT
                     CGKSPDGSTIYVVQSGDTLQEIADACGSTVEAIRELNSLTGDAIGVGQTLIVKAALEP
                     TESAAGPTPAPQEITREQVVQPQPDDWVRGPETAAVTIIEWGDFQ"
     gene            complement(1493..2701)
                     /locus_tag="AC162G_07270"
     CDS             complement(1493..2701)
                     /locus_tag="AC162G_07270"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR5075647.1"
                     /translation="MSDPRPPTVLCLASYFKGEAFLRTAKQLGCHVILVTQEQLADEP
                     WPRESIDERFLMRSLTKRPDIIYAVSYLARARNIDLIVALDDYDVLTAAALRDHMRLP
                     GMGESLARHFRDKLAMRTEAHAAGIPVPEFTGVFNHDRLREFMARMPPPWLLKPRSEA
                     SAMGIKKINHADELWPILDSLGDEQSFFVMEQYIPGEVFHVDGLVDGGQVLFNTASRY
                     DVPPMDVYHGGGLFVTRTLDRDSPDAQALLDLNPNVQRALGMVRGATHTEYIKARADG
                     RFYFLETAARVGGAHIAEAIQFATGINLWEEWARIEVAHLRVETYRLPEARRGYAGVI
                     NCLARQEWPDTSAYDDPEVVWRLKKQYHAGLIVASPDPGRVQSLLDSYSRRFVEDFLA
                     VAPPLEKAPQ"
     gene            complement(2824..3936)
                     /locus_tag="AC162G_07275"
     CDS             complement(2824..3936)
                     /locus_tag="AC162G_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HIR5075648.1"
                     /translation="MPERPSFNIGIEEEYQIVDPETRELKSYITELLEAGKIILAEQV
                     KAELHQSIVEVGTSVCDTPAEARAELVRLRRGVIELAGRKGLKIAAAGTHPFSSWVEQ
                     EITPFERYIGVKQDMQMLAQQLLIFGTHVHIGVEDPDFLIDAMNVARYMAPHVLCLST
                     SSPFWMGQDTGLKSYRSIVFRNFPRSGTPPILRTRAEFEQLVETLVKTRCIPDGSKIW
                     WDIRPNPRYPTLEFRICDVGTRVDEAICVAAIFQALIYKLWKLRQDNMTFRVYPSTLV
                     EENKWRAVRYGLDGKLIDFGKQQELPARELVRELIEWFLGDVIDELGSRAEIEYAYRI
                     LDEGSSADRQLAAYRHTGSLHAVVDQLIAETAEGVL"
     gene            complement(3940..5346)
                     /locus_tag="AC162G_07280"
     CDS             complement(3940..5346)
                     /locus_tag="AC162G_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR5075649.1"
                     /translation="MTNLREAIDFYHSLLDDETGRQSQGQMNHQLHRRELFFGERPLC
                     TVLRPRFLTVEQYDHLRRAIHTIMPAFKKVYEAARVDASIRAQLRLEDWEEQLIQSDP
                     GFRDPSPTSRMDTFFTPHPLPLSPAHAGEREEGVRGSLYFTEYNAETPAAAAYNDVLS
                     EVFYTLPVMRAFERRYEVRPLPGRHHMLHALLDAYEQWGGRDRPRIAILDWREVPTYS
                     EFVLFQDYFASQGFECIIADPREFEYRGGQLLADGILPINLIYKRVLISELVQRCGLE
                     HAVVRAVRDRAACMVNPFRCKLLHKKASLAVLSDEANAHLFDAGEQSAIDMYVPWTRA
                     LAERHTVVEGQKIDLMPFLAGHKDDFVLKPNDEYGGKGIVLGWEVSQSEWDEALRAQL
                     AEPTIVQRRVALPTEPYPSLVDGRVQIIDRMLDTNPYIWYGAYVSGCLTRLSTAALLN
                     VTAGGGSTVPTFVVEKRE"
BASE COUNT          978 a         1785 c         1835 g         1077 t
ORIGIN      
        1 cgcgccagga acacgaagct gttcacggcc agcggcgcct gcttcgcata cagctcgacg
       61 acgatgtcgc cctggtcagt ggcgatggtc gcgatgtacg tcttgttctc gtcgatgacc
      121 gtctccggat acgcgtcgta ctgcatatcc gggagctcgg cgaagtactt gaccaggccc
      181 tccagattgt tgtacgagag gtacgacagc gattcgggcc agggtgcttc gttcaccagg
      241 aggaacggcg tgccggacaa tccgatctgg accgcgagct cgcgcgccgc ctgcaccttt
      301 tgtgtgaact tgcccgaatc gagatcggcg tcgaactgct tcgcgtccag gccgatctct
      361 tcggcgtagt tgctcagctc ggtgcggaac tgatcgacgc cgagcgacgt ccaggcgtcg
      421 aacttctcga agatcacgtc atgcatctcc cagaatttcc cctgcgcgcc ggcggcctca
      481 ctggcctcgg cagacagttg cgccttgtcg tggatttcgg tgagcgggaa atgccgatag
      541 acgacacgga cgtcatccgg gtaagcgtct tccagacgct tgagcagcgg cgacaccgct
      601 gcgcagtacg gtcactggaa gtcgccccac tcgatgatcg tcacggcggc ggtctccggg
      661 ccgcgcaccc aatcgtcggg ctgcggctgg acgacctgct cgcgggtgat ctcttgcggc
      721 gccggcgtcg ggccggcggc gctctcggtc ggttccaaag cggctttgac gatcaacgtc
      781 tggccgacgc cgattgcgtc gccagtcaag ctgttcagct cgcggatcgc ttccacggtc
      841 gagccgcagg cgtcggcgat ctcttgcagc gtgtcgccgc tctgcacgac gtagatcgtc
      901 gatccatcgg gagactttcc gcaggtgatc gacggcgggg cggccgtcgg tccagctccg
      961 ctgctcgtat cggtggccgg cgtgcccgtg ctttgaggcg cgcacgcgga tacgatcagc
     1021 gcgccgatca ccgccagcca gacaaggcgt ttcatcgaac tatcatctcc tttcaactac
     1081 ggccagtcaa gacaatgggg tcgattctac catgattcgt gtattgcgta tcgcagatgg
     1141 ctgatagcgg atggctgatg gcgaatggta tatcgtggat cgccagacct ggcaggtctt
     1201 gtccgccgat gttggccgta gtctggctcg cgaagatctg ccaggtctac ttagagaatc
     1261 caacgcgcac tgcgcgcggt gtccagtcgg cggcggagct gcgtgtggag agggtctgag
     1321 ttcggcgtgg aactctgacc ccgccgcatt tccgaccgta ggtctctcga tcgacgccgg
     1381 agaccttcgg tcgtctccaa gggtgggtcg gagacgtcgc cctatcgccc gcgtttgcac
     1441 cggccattgg cgataagcga tacgctatca gcgatatgcg ataagccgcg tcttactgcg
     1501 gcgctttctc aagcggcggc gcgacggcca ggaagtcctc cacgaagcgc cggctgtagc
     1561 tgtcgaggag cgactgcacg cgcccggggt caggcgaggc gacgatcagc ccggcgtgat
     1621 actgcttctt caagcgccac acgacctccg gatcgtcgta ggccgacgtg tccggccatt
     1681 cctggcgcgc caggcagttg atcacaccgg cgtagccgcg ccgtgcctcg ggcaggcgat
     1741 acgtctcgac gcgcaggtgc gcgacttcga tcctcgccca ttcctcccac aggttgatgc
     1801 ccgtggcgaa ctggatcgcc tcggcgatgt gcgcgccgcc gacgcgcgcg gcggtctcca
     1861 ggaagtaaaa ccggccgtcg gcgcgcgcct tgatgtactc ggtgtgcgtc gcgccgcgca
     1921 ccatgcccag cgcgcgctgg acattcgggt tgagatcgag cagcgcctgc gcgtcgggcg
     1981 agtcgcgatc gagcgtgcgc gtgacgaaca ggccgccacc gtggtacaca tccatcggcg
     2041 ggacgtcgta ccggctggcg gtgttgaaca gcacctggcc cccatcgacc agcccgtcga
     2101 catggaacac ctcgcccgga atgtactgct ccatcacgaa gaaagactgc tcgtcgccga
     2161 gcgagtcgag gatcggccac agctcgtcgg catgattaat cttcttgatg cccatggcgc
     2221 tggcttcgga gcgcggtttg aggagccagg gcggcggcat gcgcgccatg aactcgcgca
     2281 gccggtcgtg gttgaagacg ccggtgaatt ccggcacggg gatgcccgcc gcgtgcgctt
     2341 ccgtgcgcat ggcgagcttg tcgcgaaagt gccgcgccag gctctcgccc atgccgggca
     2401 gacgcatgtg atcgcgcagc gcggccgccg tcagcacgtc gtagtcgtcc agcgcgacga
     2461 tcagatcgat gtttcgcgcc cgcgccaggt aacttactgc gtagatgatg tcaggccgtt
     2521 tggtcaggct gcgcatgaga aagcgctcgt cgatgctctc gcgcggccag ggttcgtcgg
     2581 cgagctgctc ctgggtgacc aggatcacgt ggcagccgag ctgcttcgcg gtccgtagaa
     2641 atgcctcgcc cttgaagtaa ctcgccaggc agagcaccgt ggggggacgg ggatcggaca
     2701 ttgccatgac tccttgggag ttagagttag aggtgggagt tagagtttga agtttggagt
     2761 ttaagctttg ggatttgaac gcccaatctc caacctctaa tctctaatct ccaatctcta
     2821 gagtcaaaga acaccctctg ccgtctcggc gatcagttgg tcgacgaccg cgtgcaggct
     2881 gccggtgtgc cggtatgcgg cgagctggcg atcggcgctc gagccttcgt ccagaatgcg
     2941 ataggcgtac tcgatctcgg cgcggctgcc cagctcgtcg atcacgtcgc cgaggaacca
     3001 ttcgatcagc tcgcgcacca gctcgcgcgc gggaagctcc tgctgcttgc cgaagtcgat
     3061 cagtttcccg tccagcccgt agcgcacggc gcgccattta ttctcctcga ccagcgtgga
     3121 cggatagacg cggaaggtca tgttgtcctg gcgcagcttc cacagcttgt agattagcgc
     3181 ctggaagatc gcggcgacgc agatcgcctc atcgacgcgg gtgcctacgt cgcagatgcg
     3241 gaactccagc gtcgggtagc ggggattggg acggatgtcc caccaaatct tcgagccgtc
     3301 ggggatgcac cgggtcttga ccagcgtttc caccagttgc tcgaactcgg cgcgcgtgcg
     3361 caggatagga ggcgtgccgg aacgcgggaa gttgcggaag acgatgctgc ggtacgactt
     3421 caggccggta tcctggccca tccagaaggg cgagctggtc gagaggcaca gcacgtgcgg
     3481 cgccatgtag cgcgcgacgt tcatcgcgtc gatcaggaaa tccgggtcct cgacgccgat
     3541 atgaacgtgc gtgccgaaga ttagcagttg ctgcgccagc atttgcatgt cctgcttgac
     3601 gccgatgtag cgctcgaacg gcgtgatctc ctgctccacc cacgacgaga agggatgcgt
     3661 gccggcggcg gcgatcttca gccccttgcg gccggccagc tctatcaccc cgcgccgcag
     3721 gcgcaccagc tccgcgcgcg cctcggccgg cgtatcgcag acactcgttc cgacttcgac
     3781 gatcgactgg tgcagctcag ccttgacttg ctcggccagg atgatcttgc ccgcttccag
     3841 cagctcggtg atgtacgact tcaattcgcg cgtttccgga tcgacgatct gatactcttc
     3901 ctcaatcccg atgttgaacg aaggtctttc gggcatggct cactctcgct tctcgacgac
     3961 gaaggtgggc acggtggagc cgccgccggc ggtgacgttg agcagcgccg ccgtggacag
     4021 ccgggtcagg cagccggaca cgtaagcgcc gtaccagatg taagggttgg tgtcgagcat
     4081 acgatcgatg atctgcacgc ggccgtcgac caggctggga tacggctcgg tgggcagcgc
     4141 cacgcggcgc tgcacgatcg tcggctcggc cagctgcgcg cgcagtgctt cgtcccattc
     4201 gctctgactg acctcccagc cgaggacgat gcccttgccg ccgtattcgt cgttcggctt
     4261 gaggacgaag tcgtccttgt gaccggcgag gaacggcatc agatcgatct tctgcccctc
     4321 gaccaccgta tgccgctcgg ccagggcacg cgtccacggc acgtacatgt cgatggcgct
     4381 ctgctcgccg gcgtcgaaga ggtgtgcatt ggcctcgtcg ctcaggacgg ccaggctggc
     4441 cttcttgtgc agcagcttgc agcggaaggg attgaccatg caagccgccc ggtcgcgcac
     4501 ggcgcgcacg acggcatgct ccagcccgca gcgctggaca agctcgctga tcaggacgcg
     4561 cttgtaaatc aggttgatcg gcaggatgcc gtcggccagg agctggccgc cgcgatattc
     4621 gaactcgcgc gggtcggcga tgatgcactc gaagccttgc gacgcgaagt agtcttgaaa
     4681 caggacgaac tcgctgtagg tcggcacctc acgccagtcg aggatggcga tgcgcggccg
     4741 gtcgcgcccg ccccactgct cgtacgcgtc gagcagcgcg tgcagcatgt gatgccggcc
     4801 aggcagcggg cgaacctcgt agcgccgctc gaacgcgcgc atgacaggca gcgtgtagaa
     4861 gacttccgac agcacgtcgt tgtaagccgc agcggcgggc gtctcggcgt tgtactcggt
     4921 gaagtacagg ctacccctca ccccttcttc cctctcccca gcatgtgctg gcgagagggg
     4981 tagagggtga ggggtaaaaa aggtgtccat gcgcgaggtg gggctgggat cgcgaaagcc
     5041 gggatcgctc tggatgagct gctcttccca gtcctccagg cgaagctgcg cgcgaatcga
     5101 ggcatcgacc ctggcggctt cgtacacctt cttgaaggcc ggcataatcg tgtggatggc
     5161 gcgtcgcagg tgatcatact gctcgacagt gaggaagcgc gggcgcagca cggtgcacag
     5221 gggccgctcg ccgaagaaca gctcccggcg gtgcaactgg tgattcatct ggccctggct
     5281 ctggcgcccg gtctcgtcat cgagcaagga gtgatagaag tcgatggctt cgcgcaaatt
     5341 tgtcatgccg atcccccgct gatggccgat agcagatggc cgatggccga tggcagatgg
     5401 cagctagctg atggctgaca gccgatggct gatagcttat cgcctattac accatccatc
     5461 acgcctcacg caacacgctc cacgcttcac gctccacgct ccacgtttta ctcaccctcc
     5521 cagaaaccca ctccacctca tctcggcacc ctgcggcttc ggctgctgcg cgagccggat
     5581 gcacatgtcg gccatgtgct cgacgcacca gcggtggaac tcgtagccga gcgaattcac
     5641 atccatgtcc ggtgcgggat tcatgaagtc gatcg
//
LOCUS       DBNHXB010000181         4335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3228797, whole genome shotgun sequence.
ACCESSION   DBNHXB010000181 DBNHXB010000000
VERSION     DBNHXB010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3228797"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="AC162G_07285"
     CDS             <1..200
                     /locus_tag="AC162G_07285"
                     /EC_number="4.4.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027718143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HIR5075650.1"
                     /translation="LHFGPDVNLGNIQPRDVLGVEALRAGLRFETLQPLVEQMEREGA
                     ADIPTLAERISRMARRRLPEA"
     gene            313..1422
                     /locus_tag="AC162G_07290"
     CDS             313..1422
                     /locus_tag="AC162G_07290"
                     /inference="COORDINATES: protein motif:HMM:NF014105.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit H family protein"
                     /protein_id="HIR5075651.1"
                     /translation="MGLFRFDTPQKVMKIGHIECGGQPGERPPVLFANMFQTGHKIVE
                     SRKPPRWDKARALDEIKLVEEMSNETGIPAVIGLVAPTEDEIKAYCEWFLSVNDTMVF
                     QIDTWTEEARRAGARFVKAAGVQHRFNYNSITAWDPDIPDQIAELKECGIKSVILQPF
                     DSEDKRASGRLKSLRRMLNEIAGGEFMSVLVDTTTMNLPTQGFCYLANRMVKEEFGLP
                     VGNAPANGSYMWKAIVERWGRPAFQGMDAAMHGIAAVMWNDWMAYGPITGTRRVFAAV
                     AAAVAMVTVMAWDEGLELPEAPNHPLNKHFPKEVEFMKGLRAAKSDRLMERQQALGEK
                     RRTSREQEQAADEASDGRATDLMPESAAAIEPDKP"
     gene            1696..2151
                     /locus_tag="AC162G_07295"
     CDS             1696..2151
                     /locus_tag="AC162G_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5075652.1"
                     /translation="MSFLQNSPHLLERAYAGTHRLLRPFRRWLKPGGRVERLFVGVEI
                     VTKRPLFDCQMCGHCVLHSTGMTCPMTCPKNLRNGPCGGVRPDGRCEIIPDMMCVWVQ
                     AWERSQRMPGYGSDILKVLPPLNRQLSGTSAWINDLAGIAQKLPAGWSE"
     gene            2094..3098
                     /locus_tag="AC162G_07300"
     CDS             2094..3098
                     /locus_tag="AC162G_07300"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR5075653.1"
                     /translation="MDQRPGGNRAEASGGLERMRSGSRLERVLRSGRFAVTAELNPPD
                     SADPQSVYRRALVLAEVCDAINATDASGANVHMSSVGMCAVLTHAGYEPVMQVSCRDR
                     NRIAIQGDLLGAAAMGVRNVLCLTGDGVQVGDQPEAKPVFDLDSISLLRTACILRDKG
                     QFLSGRKLESPPRLFLGAAENPFAPPYDFRPLRLGKKIEAGADFIQTQYCFDVPRLRE
                     FMARVRDLGLHERAFILIGVGPLRSAKAAEWMQAHVPGVVIPDEIIRRLKGAPAGRQW
                     QEGKRVCIEIIQQVREIEGVSGVHVMAYRQEELVAEIIREAGLLRRRHLREKEPRGGM
                     "
     gene            3158..>4335
                     /locus_tag="AC162G_07305"
     CDS             3158..>4335
                     /locus_tag="AC162G_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012432.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR5075654.1"
                     /translation="MTATGNAETESRLTEPPAPELIASAYTREVGDAAILYEGMSLAD
                     MAHVVALIESDIIPRQAGGELLSALLDIHPSPPLDFALDPARGDLYSNREVYLRMLTS
                     AAGWLSAGRARREATTIGYRIALRRRLLAWAAALEDGAVAALDQAEAHRATLFPDYTY
                     LQPAQPTTFGHYLLTFVYPMLRDLDRTRAAFARANVSPAGSGSVNGSRLPLDRARLAN
                     LLGFDAVIPHTRDAMWQADGPIEIAALVAAALVNLDRLAEDLQIFSTAEFSLVQLADR
                     HTRTSVIMPQKKNPYSLAYVRGVCGETIGALAAMAAVGKTPSGQIDNRIFAYGDVPRT
                     LDSAIGAAHLTAGVLRGLRVNAEHAARRVAQDFIGATDLAEVIMLKTGLDYKTAHDVV
                     G"
BASE COUNT          781 a         1426 c         1404 g          724 t
ORIGIN      
        1 tcctgcactt cggcccggac gtgaacctgg gcaatatcca gccgcgcgac gtgctcggcg
       61 tcgaagccct tcgcgccggc ctgcgcttcg agactttgca gcctctcgtc gagcaaatgg
      121 agcgcgaagg agccgcagac atcccgacac ttgccgagcg catctcgcgg atggcgcgcc
      181 gccgcttgcc ggaagcatga gactcgccta tcgctgatgg cttatcgcgt atcgccgatg
      241 acatgtgccc gtcagaggtg ggcaatacgc gatatgcaat aagcgatacg ccccacacat
      301 ggagcacagg ctatgggatt attccgcttt gacactcccc agaaagtgat gaagatcggc
      361 cacatcgagt gcggcggcca gccgggcgag cggccgccgg tgctgttcgc caatatgttc
      421 cagaccggcc acaagatcgt cgagagccgc aagccgcccc gctgggataa ggccagggcg
      481 ctcgacgaga tcaagctcgt cgaggagatg tcgaacgaga cgggcatccc tgccgtcatc
      541 ggcctcgtcg cgccgacgga ggacgagatc aaagcctact gcgagtggtt cctctcggtc
      601 aacgacacga tggtcttcca gatcgacacg tggaccgaag aagcccgccg cgccggggcg
      661 cgcttcgtga aagcggccgg cgtgcagcac cgcttcaact acaacagcat taccgcctgg
      721 gaccctgaca ttcccgatca gatcgccgag ctgaaggagt gcggcatcaa gtcggtgatt
      781 ctccagccct tcgacagcga ggataagcgc gccagcggcc gcttgaagtc tctgcgaagg
      841 atgctgaacg agatcgcggg cggcgagttc atgtccgtgc tggtggatac gacgacgatg
      901 aatctgccga cgcaggggtt ctgttacctg gcgaatcgca tggtcaaaga ggagttcggt
      961 ctgccggtcg gcaacgcgcc cgccaacggc tcctacatgt ggaaggcgat tgtcgagaga
     1021 tggggccgcc ccgcatttca aggcatggac gccgccatgc acggcatcgc cgcagtgatg
     1081 tggaacgact ggatggccta tggcccgatc accggcacgc gccgggtgtt cgccgccgtc
     1141 gccgccgccg tggcgatggt gacggtgatg gcgtgggacg agggcctcga actgcccgaa
     1201 gcgcccaatc acccactcaa caagcatttc ccgaaagaag tcgagttcat gaagggcctg
     1261 cgcgccgcga agagcgaccg actcatggag aggcagcagg cgcttggcga gaagcgccgc
     1321 acatcgcgcg agcaggagca agccgcagat gaggccagcg atggtcgggc cacagatctc
     1381 atgcccgaga gcgcggcagc catcgagccc gacaaacctt aaccacgaag gcgctaagcg
     1441 cctatccgaa agcgaccgga gtgcggactg ccaggctccg gccgccgtcc ccggcggcgt
     1501 tggttgcgcc gaggatagcg ggggaaagat gcctcgtcgt tcctcctcgg catgacacat
     1561 ctggcgcgga tctagtggta cgtccgtatg tgccagaccg tatggcgtgc cttgctacgg
     1621 aggactcgcg atgatgcctg agtagctgcg gcacggtgaa caattactgg ctggcgtcga
     1681 ggtcagagag gcagtgtgag cttcttgcag aattcgccgc acctgctaga gcgagcgtac
     1741 gcgggcacgc accggctgct gcgccccttt cgccgctggc tgaagccggg cgggcgggtc
     1801 gagcgtctgt ttgtcggagt tgagatcgtc acgaaaaggc cgctcttcga ctgccagatg
     1861 tgtggccatt gtgtcctgca cagcaccggc atgacctgcc cgatgacctg ccccaagaat
     1921 cttcgcaacg gcccctgcgg cggtgtgcgg cccgacgggc gctgcgagat catccctgac
     1981 atgatgtgcg tctgggtgca ggcatgggag cgctcgcagc gcatgccggg ttacggaagc
     2041 gacattctca aggtgctgcc gcccctcaat cgccaactgt cgggcacgtc cgcatggatc
     2101 aacgacctgg cgggaatcgc gcagaagctt ccggcgggct ggagcgaatg aggtccggct
     2161 cgcggctgga gcgcgtgctg cgctcgggcc gtttcgccgt caccgccgag ctgaacccgc
     2221 cggacagtgc tgacccgcag tcggtctacc gccgggcact ggtcttggcc gaggtatgcg
     2281 acgcgatcaa cgcgaccgac gcctccggcg cgaacgtcca catgtcgagt gtgggcatgt
     2341 gcgctgtgct cacccacgcc ggctacgagc cagtgatgca agtgtcttgc cgggaccgca
     2401 accgcatcgc catccaaggc gacctgttgg gcgcggcggc gatgggcgtg cgcaacgtgt
     2461 tgtgcctcac cggcgatggc gttcaggtcg gcgatcagcc cgaggccaag cctgtctttg
     2521 acctcgattc gatctccctg ctgcgcaccg cgtgtatcct gcgcgacaag gggcagttcc
     2581 tcagcgggcg aaaactggaa tcgcccccca ggctgttcct gggcgcggcg gaaaacccat
     2641 ttgccccgcc gtacgatttt cgccctttgc ggctgggcaa aaagatcgag gcgggcgcgg
     2701 atttcatcca gacgcagtac tgcttcgacg tgccgcgcct gcgcgagttc atggctcgcg
     2761 tcagagacct gggcctgcac gagcgcgcct ttattctgat cggcgtcggg ccgctgcgct
     2821 cggccaaggc cgccgagtgg atgcaggcgc atgtccccgg cgtcgtgatt ccggacgaga
     2881 tcatccgtcg attgaagggc gcaccggcag gccggcagtg gcaggaaggc aagcgcgtat
     2941 gcatcgagat catccagcaa gtgcgtgaga tcgagggcgt cagcggcgtg cacgtcatgg
     3001 cctaccgcca ggaagagctt gtcgcggaga tcatccggga ggcgggcctg ctgcgccggc
     3061 gccacctgcg ggagaaggaa ccgaggggcg gcatgtaggc ttagccgaat agattgacca
     3121 cccgacgaag tcatgcgaga attagacgca acccgacatg actgccaccg gaaacgccga
     3181 gaccgaatcc cgcctgactg agccgcccgc gcctgagttg atcgcttcgg cgtacacacg
     3241 cgaggtcggc gatgcggcca ttctctacga aggcatgagc ctggccgaca tggcgcacgt
     3301 cgtcgcgctg atcgaaagcg acatcatccc gcgccaggcc gggggcgagt tgctctccgc
     3361 gctgcttgat attcacccat ccccgccgct cgacttcgcg ctcgacccgg ctcggggcga
     3421 cctgtattcc aaccgcgaag tctatctgcg aatgcttacc tcggccgccg gttggctctc
     3481 ggcgggacgc gcccgccggg aggccacgac gatcggctac cgcatcgccc ttcgccggcg
     3541 acttctcgcg tgggctgctg cgctcgaaga tggcgccgtc gccgcgctcg accaggctga
     3601 agcgcaccgc gccacactgt tccccgacta cacctacctg cagccggccc agcccacgac
     3661 cttcgggcat tacctgctca cctttgttta tcccatgctg cgcgacctcg accggacacg
     3721 cgccgccttc gctcgtgcca acgtcagtcc cgccgggtcg ggcagcgtca acggcagccg
     3781 gctcccactc gaccgcgcgc gcctggccaa ccttctcggc tttgatgccg tcatccccca
     3841 cactcgtgat gcgatgtggc aggccgacgg cccgattgag atcgccgcgc tcgtcgctgc
     3901 cgcgctcgtc aacctcgatc gcctggccga ggatttgcaa atcttctcca cggctgagtt
     3961 cagcctcgtc cagctggccg accgccacac ccgtacgagc gtaatcatgc cgcagaagaa
     4021 gaatccgtac tcgctggcct acgtgcgggg cgtctgcggc gaaacgatcg gcgcgctcgc
     4081 ggcgatggcc gccgtcggca agacgccctc gggccagatc gacaaccgca tattcgccta
     4141 cggcgacgtt ccgcgcacgc tcgacagcgc gatcggggcc gcacacctga cggctggcgt
     4201 tttgcgtggc ctgcgcgtga atgccgaaca tgccgcccgg cgcgttgcgc aggacttcat
     4261 cggcgcgacc gacctggccg aagtgatcat gctgaaaacc ggcctcgact acaagaccgc
     4321 gcacgatgtc gtcgg
//
LOCUS       DBNHXB010000182        11905 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_377414, whole genome shotgun sequence.
ACCESSION   DBNHXB010000182 DBNHXB010000000
VERSION     DBNHXB010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11905
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_377414"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..2595)
                     /locus_tag="AC162G_07310"
     CDS             complement(64..2595)
                     /locus_tag="AC162G_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5075655.1"
                     /translation="MHDATKPDHIKQLTTRIARLGAQSRRARVMLALGSVGGYALLFF
                     ALHPVMGGAAAALVILPVAAIGWLWGMWAGLLAGLLSIMLNTLLLNLAGEAGWDVVFR
                     TGGGPGFVTLALIGAAVGRLRDLSDRVKLQLAERERAEEALRDTTAALNSTLNLEEVL
                     DRILANVGRVVPHDAASVMLIEASVSYLVRRVGYMDPVAGSGVRSLRFRVADVPTLRE
                     MSESGQPLVIPDTRTYTGWVDRPETHWIRSYVGAPIRMKGQTIGFLNLNSAAPGFFTA
                     THAERLLVFADHTAIAIDNARLYGAERRRVETLAALHEAGLALNTQLDLPVLLQTIVE
                     RAARLLGSPMGELYLLLPDEQTLELKAGYNLPGELTGLRLRLGQGFSGRIAQTGEPLI
                     VGNYNTWLGRLTAFDHIPFGSVVGAPIKWQERVTGVILVDDFQPERFEPADVQSLSFF
                     ANQAAIAIENAQLYDAIRRHAGELEQRVIERTAELDRERQRLATILDATGEGIALIDR
                     NHVIVYVNAAMEQLTGYTLAEVLNHTIDLWSHEHTPPAVNEALERCFERGEPWRGEVI
                     SGRKDGTHYDAALTIIPLRGADGAIVGYVVSQRDITHLKELERLRNQFVSRIGHELRT
                     PLSNIRLYVELLERGKPEKRAEYMKTLWRETARLQKLIEGFLEISQLDAGMLPVNPVP
                     TDLNQLLGRVIGEHSALAAERGLTLTLHPDPGLPRVLADPELVDQVIANLLENALRYT
                     PRGGRIAVRTALRRREEREWVTLAVQDTGPGISPEEMPRLFGRFYRGEAARDFSTPGA
                     GLGLAISKAIVDRLEGRITVDGGVEGEPPQRQGATFVVWLRPAMG"
     gene            3069..3719
                     /locus_tag="AC162G_07315"
     CDS             3069..3719
                     /locus_tag="AC162G_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5075656.1"
                     /translation="MVNRSNEDWLRALSEQPGEAQAEALQDLRDFLVRAVLVYLSLHR
                     TELVNWSREDVRGLAEDLAQDALIEIRASLDSFRGDSKFTTWAYRFVINRAASELRRR
                     RYQNQSLDGLREQEPALFQAILSDQDRVEPEQLADQRYYLDLLREIIETELSERQRTA
                     LIAIHWHGRSMDEVAEMMDLTRNALYKLLHDARKRIKASLQARHLSGGDILAPFQE"
     gene            3799..4095
                     /locus_tag="AC162G_07320"
     CDS             3799..4095
                     /locus_tag="AC162G_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075657.1"
                     /translation="MNYTEFTEWLERIYATAETEIDCEQFQALLPAYVDFEIAGQAAA
                     LYLAQAKAHLAQCPDCAEEYAGLRKVAELEARGRLPKVEESLKPFQESPALEPV"
     gene            4454..6493
                     /locus_tag="AC162G_07325"
     CDS             4454..6493
                     /locus_tag="AC162G_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HIR5075658.1"
                     /translation="MDTGNTLLAKIEQMQDTTGFRSLHWEGSFEDYLQIVRNDPKVAR
                     TAFQRIYDMIVSYGYDEYTRNRETLVHYRFFDDPFENGKDAVFGLDKPLMELGRIFQS
                     AARHYGTERRVMLLHGPVGTAKSTVVRLLKKGLEAYTRTDAGRLYSFYWLPDDGDRMD
                     CPMHEEPLHLVPLEFRPAVLEDINGGHGELDRIEIEGDLCPACRYMYNRLLEKSGGNW
                     LDVVRQARVKRLLLTEKDRVGIGTFQPKDEKNQDSTELTGDINYRKIAEYGSDSDPRA
                     FNFDGELNIANRGLVEFIEILKLDVAFLYDLLTASQEHKIKPKKFAHTDIDEVILGHT
                     NEAEYRRLLSNEYMEAMRDRTLKIDIPYVIRYSDEVKIYERDFNDRRVVGKHIAPHTL
                     EIAALWAVLTRLEEPKHAGLTLLQKLKLYDGRSLPGFTEDSVLELQAEAKQEGMLGIS
                     PRYIQDKLSNALVSDQSRTCINPFLLMRELEGGLKHHSLITSEEQRKHFRELLAVARA
                     EYDEIVKNEVQRAITADEAAIKRLAANYIDNIKAYTQKQKVRNPYTGQDEPPDERLMR
                     SIEEKIEIPESRKDDFRREIMNYIGALAIEGKTFSWDSNDRLRRALELKLFEDQKDSI
                     KLTSLVSNVIDSETQEKIEVVKSRMIKNMGYCDVCATDVLNYVASIFARGDTARK"
     gene            6568..7662
                     /locus_tag="AC162G_07330"
     CDS             6568..7662
                     /locus_tag="AC162G_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HIR5075659.1"
                     /translation="MILSIEQDTSRFRQIVRGKIKQNLRKYISNGELIGRQGKDLVSI
                     PIPEIELPHFRFGGRQGGVGQGPGDIGTPLGPGEPGDGNGKAGDQPGAHILEVELTLQ
                     ELVELLGEELELPRIQPRSKNNVDGEVTRYTGIRRVGPEGLRHFKRTYREALKRQMIS
                     GTYNYDRPAILPIRDDKRYRSWKVYPRPNSNAAVIYMMDVSGSMTNRKKHLVRLTAFW
                     IDTWLRAHYKNLVTRYIVHDAVAHEVDQHTFYHVRESGGTRISSAYELCRDIMRRDYP
                     VEDWNLYALHFSDGENYGDDDDERCIALLGHDLLPKTNLFCYGEVQSSSGRKFMDTLD
                     DVDDEKLATAAIDDDDDIYEAIKTFLGKGV"
     gene            7724..9199
                     /locus_tag="AC162G_07335"
     CDS             7724..9199
                     /locus_tag="AC162G_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HIR5075660.1"
                     /translation="MSLSPDLQQLQTTIEGYAREFGLDFFEVRFEMLDYRMMNQVAAY
                     DGFPTRYPHWRFGMEYERLSKSYAYGLHRIYEMVINTNPCYAYLLASNQRVDQKLVMA
                     HVFGHSDFFKNNLWFAHTNRRMLDEMANHAARIRRHVERHGLDCVEDFLDACLSLDNL
                     IDIHSVGIRRRPLPSSEDEEPKTVHKLPAKAYMDAYINPPEFLAEQQRQLEEAEQHRK
                     HFPEEPERDVLLFLLEHAPLDNWQHDVLDIVREEAYYFAPQRQTKIMNEGWASFAHTR
                     IMTERALEPDELIDYAEHHSGTVAVHPGRLNPYKLGLELYRDIEDRWNRGAFGPEYEA
                     CESMQARREWDRKLGLGRQKVFEVRRVYNDIGFMDEFLTEDFAREHQLFTFDYNEDDG
                     QYEIASREFQEIKRRLLFQLTNFGQPIVEIADGNYQNRGELYLVHRHEGIDLDVPFAQ
                     DTLRNLYTLWGRPVHVETQVEDEGRVLFSHGPDGGARRNLN"
     gene            9220..10344
                     /locus_tag="AC162G_07340"
     CDS             9220..10344
                     /locus_tag="AC162G_07340"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR5075661.1"
                     /translation="MSQLDPERQQQAKIYARIRRRLFLVDVLIGGAYALAWLILGWST
                     SLKASLLTVTTDNWLLVALFGAVFGGIYYLINLPLSYTSGFVLPHRFGLSAQTRKGWI
                     GDQVKILSLGAILGGLILEVVYAVLRAAPETWWLWAAGLLLLFNVVLANLAPILIAPL
                     FFKFVPLGEEHADLAQRLIRLAGRANTKVRGVFKFDMSRRTKAANAALMGLGNTRRIV
                     LGDTLIEGFTPDEIETVLAHELGHHVNKDIPLGILVETALTVGGLYLASLALNWGVAA
                     FGFGGPADVAALPLFALVMGAYGLITMPLGNAYSRWRERRADEYALRATGNGPAYASA
                     LTRLANQNLAEVDPEPWVEFLFYSHPALGKRIAMARASGE"
     gene            10337..11032
                     /locus_tag="AC162G_07345"
     CDS             10337..11032
                     /locus_tag="AC162G_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR5075662.1"
                     /translation="MSEGRPWNELNTAIMACEKCPRLRAYCANIAQEKRRAFRDQTYW
                     GRPVPGWGDPNARILIVGLAPAAHGANRTGRMFTGDRSGSFLYSALYRTGLANQPTSV
                     SRDDGLKLTDVYITAATRCAPPDNKPTPGELHNCRPYLVEELRLLQRARVIVPLGRIA
                     FDNSLRALEAVGASVLSPRPRFAHGATCRVETCHVHTLIASYHPSRRNTQTGLLTPVM
                     FDRIFQHARALAR"
     gene            11091..>11905
                     /locus_tag="AC162G_07350"
     CDS             11091..>11905
                     /locus_tag="AC162G_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR5075663.1"
                     /translation="MRLFPLLCAIALFAAPIAASAQSDAVSDLLNRINALRRQNGLAP
                     LELNDALNAAAQRHSLDMANTGNVDHTGSDGSTPEQRIDAAGYAPRYTWGENIYGGGI
                     ATVDDAWAFWTSSTVHRNNLLNERYREVGIGAAAGANGTYYTLTFGARPGVLPFFVDQ
                     GSVVDSPAVTLTLSNEEAEPVGSGGTMGRAVEIRVGEGEVLSAVAWQPWQRTIPFTLS
                     SVDGEHRIRVEYRDANGVVASYFRLVTLTGAAAPSTATPTTIPTVQPASTHTAT"
BASE COUNT         2349 a         3820 c         3637 g         2099 t
ORIGIN      
        1 gcccctcgtg ggcgtcgttt ccgccgaaaa gcgggcacaa ggccctatgc tacaggtaga
       61 tagtcaaccc atcgccggtc taagccacac cacgaaagtc gcgccctggc gctgaggtgg
      121 ctctccctca actcccccat cgacggtgat gcgaccctcc aggcgatcga cgatggcctt
      181 gctgatagcc agccccaacc cggcgccggg cgtgctgaaa tcgcgcgcag cctcgccccg
      241 gtagaaacgc ccgaacaggc gcggcatctc ttcgggtgag atgcccggcc cggtgtcttg
      301 caccgccagc gtgacccact cgcgctcctc tcggcggcgg agagctgtgc gcacggcgat
      361 gcgcccgccg cgcggcgtgt agcggagcgc gttctctagc agattggcta tgacctgatc
      421 caccagctcc ggatccgcca gcacgcgtgg cagaccggga tcgggatgaa gcgtgagcgt
      481 taagccgcgc tcggcggcca gcgcgctgtg ctcgccgatc acccggccga ggagttgatt
      541 gagatcggtc gggaccggat tgaccgggag catgccggcg tcgagctgcg aaatctcgag
      601 gaagccctcg atcagcttct gcaggcgggc ggtttcgcgc cacagcgtct tcatgtattc
      661 ggcacgcttc tccggcttgc cgcgctccag cagctcgaca tacagcctga tgttggatag
      721 cggcgtgcgc agttcgtggc cgatgcgcga gacgaactga ttcctgagcc gctcgagttc
      781 cttgaggtgc gtgatgtcgc gctgcgatac gacgtagccg acgatggcgc cgtcggcgcc
      841 ccggagcggg atgatcgtca acgcggcgtc gtagtgtgtg ccatctttgc gcccgctgat
      901 cacctcgccg cgccacggct cgcctcgctc gaagcagcgc tctagcgctt cgttcacggc
      961 cggcggcgta tgctcgtggc tccataggtc gatcgtgtga ttcagcactt cggcgagcgt
     1021 gtagccggtg agctgctcca tcgcggcatt gacgtagacg atgacatggt tacgatcgat
     1081 cagggcgatg ccttcgccgg tcgcgtcgag gattgtcgcc aggcgctggc gctcgcgatc
     1141 gagttcggcg gtgcgctcga tcacgcgctg ctccagctcg ccggcgtggc ggcggatcgc
     1201 gtcgtacaac tgcgcgttct cgatggcgat cgcagcctgg ttggcgaaga agctcagcga
     1261 ttgtacgtcc gccggttcga agcgttcggg ttgaaaatcg tcgacgagga tgacaccggt
     1321 gacgcgctct tgccacttga tcggcgcacc caccacggag ccaaaaggta tgtggtcgaa
     1381 ggctgtcaat cgccccagcc aggtattgta gtttcctaca atcagcggct cccccgtctg
     1441 ggcgatgcga ccggagaagc cttggcccag gcgcaagcga agacctgtca gttctccggg
     1501 cagattgtaa cctgccttca gttccagcgt ctgctcgtcc ggcagcaaca agtagagttc
     1561 tcccattggg ctgcccagca agcgcgcggc gcgctcgacg atggtctgca gcagcacagg
     1621 caggtcgagc tgcgtgttca gagcaaggcc ggcctcgtgc agcgcagcca gcgtctcgac
     1681 gcggcggcgc tcggcgccat acagcctggc gttgtcgatg gcgatagcag tatggtcagc
     1741 gaaaacgagc aggcgctcgg cgtgcgttgc cgtgaagaaa ccgggcgcgg cgctgttgag
     1801 gttgaggaaa ccgatcgtct gtcccttcat gcgtatcggc gcgccgacgt acgagcgtat
     1861 ccagtgcgtc tccgggcggt cgacccagcc ggtataggtc cgggtatccg gaatgaccag
     1921 cggctggccg gattcgctca tctcacgcaa tgtcggcacg tcggccaccc ggaagcgcag
     1981 ggagcgcacg cccgaccccg caaccgggtc catgtagccc acacggcgga cgagatagga
     2041 tacgctcgct tcgatcagca tcacactggc cgcgtcgtgc ggcacgaccc gcccgacgtt
     2101 ggccaggatg cggtcgagca cttcctcgag gttgagcgtg ctgttgagtg cggccgtcgt
     2161 gtcgcgcaga gcctcctctg ctcgctcgcg ttcggcaagt tgcagtttca cccggtcgct
     2221 cagatcgcgc agccgcccga cggccgcgcc gatcagcgcc agcgtcacga atcccggtcc
     2281 gcctccggta cgaaacacta cgtcccaacc ggcctccccg gccaggttca acaggagcgt
     2341 attcagcatg atactgagca gcccggccag cagccccgcc cacatccccc agagccaccc
     2401 gatcgcagcc accggtaata tgaccagcgc ggccgccgcg ccgcccatca cgggatgaag
     2461 cgcgaaaaac agcagtgcgt agccgcctac actgccgagc gcaagcatca cccgtgctcg
     2521 gcgggactgc gcgccgaggc gtgcgatgcg tgtggtgagc tgtttgatgt gatcgggttt
     2581 tgtcgcgtcg tgcatcgcag gcttggaagc tgtctcaaac tcgcgcaaag gcgcaaaacc
     2641 gctaagaatt ccggacaaga ctttgcgcct gagcatccct tcggccctgc tcggggcagg
     2701 cttggcgtga ggcttttcag acaggccctt cgaggtgaaa ccaaccacgt gcgatgcact
     2761 ctagagtgag tcgcacgttc ttgcaccttt aaccctcacc gttgagtcta tgtcaacttt
     2821 ctcccggagt caagaagcgg ttgcagaagc cctgacctgc ccttccgggt ctcttgccga
     2881 ccgtggtata tttatactgc tcgcacctgt aatcctgagc catagacctg gcaggtttgt
     2941 ccgccggtgt cagtcgcggg ttcgccccgc gttgcgaagg cctgccacgt ctacttggag
     3001 aatccgggtg cgtatgggat aatggcgacg gggcatggat cgttcgtcgc agacgaaggg
     3061 ccggcaagat ggtgaatcgc tcgaacgaag actggctgcg ggcgctctct gaacagcccg
     3121 gcgaggcaca ggccgaagcg ttgcaggact tgcgcgactt cctcgtgcgc gctgtcctcg
     3181 tctatctctc cctgcatcgc accgagctgg tcaactggag ccgggaggat gtgcgcggcc
     3241 tggcggaaga cctggcgcag gatgcgctga tcgagatccg cgctagcctg gatagcttcc
     3301 ggggtgattc gaagttcact acctgggctt accgcttcgt catcaaccgg gcggcgagcg
     3361 agctgcgccg acggcgttat cagaatcaat cgctcgatgg cctgcgcgag caagagccgg
     3421 ccctcttcca ggccattttg agcgaccagg acagggtgga gccggagcag ttggccgacc
     3481 agcgttacta cctggatctg ctccgcgaga tcatcgagac cgagttgagc gagcgccagc
     3541 gcacggcact gatcgccatc cactggcacg gccgttcgat ggacgaagtc gcggagatga
     3601 tggacctcac tcgcaacgcg ctgtataagc tgcttcacga tgcccgcaag cgcatcaagg
     3661 cgagcctaca ggcccgccac ttgagcgggg gcgatatcct ggcccccttt caagagtgac
     3721 catagaaaac ctgtaagaaa tcgcctactg agagcgtctt acctcttggt aaagagatac
     3781 cccacaagga gcgcaccatt gaactataca gaattcaccg aatggctcga gcgcatctat
     3841 gcgactgccg agaccgaaat agattgcgag caatttcagg cgctcctgcc ggcctacgtc
     3901 gatttcgaga tcgccggtca ggccgcagcc ctttacctgg cgcaggctaa agcgcacctg
     3961 gctcagtgtc cggactgcgc tgaagaatac gcgggccttc gcaaagtggc cgaactcgaa
     4021 gcccggggcc gtctgccgaa agtcgaagag agtctcaagc cattccagga gagtcccgca
     4081 ctcgagcccg tttgaatgtg atacaacgcg cgacgcacct ctgaccctct ccgctttcct
     4141 tcggctgcgc tcaggacagg cgcagcgaga gcaagagtgc agcgtcacat ttaggcctat
     4201 ccgaaaagtg aagtgcagac tttcgtctgc atggccaaag cgggctaaag ccccgcaggc
     4261 cgggattttc ggataggctc tttgggattg tcaagataag aagcccggtt tctaggaagg
     4321 aaccgggttt tgttgttggc ccaaatatcc cacaccaatt acttacacaa ccccaacgaa
     4381 cgtcacgtaa gaacaaatcg tatcgcaacg tcccacctct caacagcagc gacgcgggtt
     4441 gaggaggcag gcaatggata caggcaacac gcttctggcc aagatcgagc agatgcagga
     4501 cacgaccgga tttcgcagcc ttcactggga aggcagtttc gaggattacc tccaaatcgt
     4561 gcgaaacgat cccaaagtcg cgcgcacggc cttccagcgt atttacgaca tgatcgtctc
     4621 ttatggttac gatgaataca cgcgcaaccg cgagacgctc gtccactatc gtttcttcga
     4681 cgaccccttc gagaacggca aagatgccgt cttcggcctc gacaagccgc tgatggagct
     4741 agggcgcatc ttccaatcgg cggcgcgcca ctacgggacc gagcggcgcg tgatgctgct
     4801 gcacggcccg gtcggcacgg ccaagagcac ggtcgtccgc ctgctcaaga agggactgga
     4861 ggcctacacg cgaaccgatg ccggccgcct gtactccttc tactggctgc ccgacgacgg
     4921 cgatcggatg gattgcccga tgcacgagga gccgctgcac ctggttcctc tggaattccg
     4981 gccggccgtg ctggaggata tcaacggggg gcacggcgag ctagatcgga tcgaaattga
     5041 gggcgatctg tgcccggcct gccgttacat gtacaaccgc cttctggaga agtctggcgg
     5101 caactggctc gacgtcgtcc gccaggcgcg cgtcaagcgg ctgctgctga cggagaaaga
     5161 tcgggtgggc atcggcacct tccagcccaa ggatgagaag aatcaggact cgaccgagct
     5221 gaccggcgac atcaactacc gcaagatcgc cgaatacgga tcggattctg atccgcgcgc
     5281 cttcaatttc gacggcgagc tgaacattgc caatcgcgga ctcgtcgaat tcattgaaat
     5341 cctcaagctc gacgtggctt tcctctacga cctgcttacc gcctcgcagg agcacaagat
     5401 caagcccaag aagtttgcgc acacagacat cgacgaagtg atcttgggcc acacgaacga
     5461 ggccgaatac cgccgcttgc tgagcaacga gtacatggag gcgatgcgcg accgcacgct
     5521 caagatcgac attccttacg tcatccgcta cagcgacgaa gtgaagattt acgagcgcga
     5581 cttcaacgat cggcgcgtcg tcggcaagca catcgcgccg cacacgctgg agatcgccgc
     5641 gctgtgggcg gtgctgaccc ggctggagga acccaagcac gccgggctga cgctgctcca
     5701 gaagctgaag ctgtacgacg gccgctcgct gcccggcttc accgaagaca gcgtgctgga
     5761 gctgcaggcc gaagccaagc aagaaggcat gctcggcatc tcgccgcgct acatccagga
     5821 caagctgtcc aacgcgctgg tcagcgatca gagccgcacc tgcatcaacc ccttcctgct
     5881 catgcgcgag ctggagggcg gcctcaaaca ccactcgttg atcacgagcg aggagcagcg
     5941 caagcacttc cgcgagctgc tggccgtggc gcgcgcggag tacgacgaga tcgtcaagaa
     6001 cgaagtgcag cgcgccatca cggccgacga ggctgccatc aagcgcctgg ctgccaacta
     6061 catcgacaat atcaaggctt acacccagaa gcaaaaggtg cgcaacccgt acaccgggca
     6121 ggacgagccg ccggacgagc gactgatgcg ctcgatcgaa gaaaagatcg aaatccccga
     6181 gagccgcaag gacgactttc ggcgtgagat catgaattac attggcgcgc tggcgatcga
     6241 gggcaagacg ttctcctggg actcgaacga tcgtctgcgc cgcgcgctcg agctcaagct
     6301 gttcgaggat caaaaggatt ccatcaagct gaccagtctg gtatccaacg tgatcgacag
     6361 cgagacgcag gagaagatcg aggtcgtcaa gagccgcatg atcaagaaca tgggctactg
     6421 cgacgtgtgc gcgacggacg tgctcaacta cgtcgccagc attttcgccc ggggcgacac
     6481 agcccggaag tgaaacgtaa cgagtaaaac gtaaattgtg acgttttacg tttcacgttt
     6541 tacgatttta tcttcaggaa tacagccatg atactctcca tcgagcaaga caccagccgc
     6601 ttccggcaga tcgtgcgcgg caagatcaag caaaacttgc gcaagtacat ctccaacggc
     6661 gaattgatcg gccgccaggg gaaagacctg gtgagtattc ctatccccga gatcgagctg
     6721 ccgcacttcc ggtttggcgg tcggcagggc ggcgtcgggc aggggccggg cgatatcggc
     6781 acgccgctcg gcccgggcga gccgggcgac ggcaatggca aggccggcga tcagcccggc
     6841 gcgcatatcc tcgaagtcga gttgacgctg caagagctgg tcgagctgtt aggcgaagaa
     6901 cttgagctgc cgcgcatcca gcccaggagc aagaacaacg tcgacggcga agtcactcgc
     6961 tacactggca ttcggcgcgt cggcccggag ggcctgcgac atttcaagcg cacgtaccgc
     7021 gaggcgctca aacggcagat gatctccggc acgtacaatt acgaccgccc ggccatcctg
     7081 cccatccgcg acgacaaacg ctaccgctcg tggaaggtct acccgcgccc gaatagcaac
     7141 gcggcggtga tctacatgat ggacgtgtca ggcagcatga ccaatcgcaa gaagcacctg
     7201 gtgcggctga ccgccttctg gatcgatacc tggctgcgcg cgcactacaa gaatctggtg
     7261 acccgctata tcgtccacga cgccgtcgcg cacgaggtgg accagcacac gttctaccat
     7321 gtgcgcgaga gcggcggcac gcgcatttct tccgcctacg aattgtgccg cgacatcatg
     7381 cgccgggatt acccggtcga agactggaac ctgtatgccc tgcacttctc cgacggcgag
     7441 aactacgggg acgacgacga cgagcggtgc atcgcgctgc tggggcacga tctgctgccg
     7501 aagacgaacc tgttctgcta cggcgaggtt caaagctcgt ccgggcgcaa gttcatggac
     7561 acgctcgacg acgtggacga cgaaaagctc gccacggccg ccatcgacga cgacgacgac
     7621 atttacgagg ccatcaaaac atttctggga aagggtgtgt gaacgtaaag cgtgaagcgt
     7681 aaaacgtaat tacgttttac gctttacgtt ttacgcaaca gccatgtctc tctctcccga
     7741 tcttcagcaa ctccaaacca ccatcgaagg ctacgcccgc gaattcggcc tcgacttctt
     7801 cgaggttcgc ttcgagatgc tcgactaccg gatgatgaac caggtggccg cctacgacgg
     7861 gttccccacg cgttacccgc actggcgctt cggcatggaa tacgagcgcc tgtccaaatc
     7921 gtacgcctac ggcctgcacc gcatctacga gatggtgatc aacaccaatc catgctacgc
     7981 ctacctgctg gccagcaacc agcgcgtgga tcagaagctg gtcatggcgc acgtattcgg
     8041 ccattccgat ttcttcaaga ataacctgtg gttcgcgcac accaaccgca gaatgctcga
     8101 cgagatggcg aaccacgcgg cgcgcatccg ccggcacgtg gagcggcacg ggctggactg
     8161 tgtcgaggat ttcctggacg cgtgcctctc gctcgacaac ctgatcgaca ttcattcggt
     8221 cggcattcgc cgtcgccctc tgccgtcatc tgaggatgaa gagccgaaga ctgtgcacaa
     8281 gctgcccgcc aaagcctaca tggacgcata tatcaatcca ccggagttcc tggccgagca
     8341 gcagcgccag ttggaggagg ctgagcagca tcgcaagcac ttccccgaag agccggagcg
     8401 cgacgtgctg ctgttcctgc tcgaacacgc gccgctggac aactggcagc atgacgtgct
     8461 cgacatcgtg cgcgaggagg cgtactactt cgcgccgcag cggcagacga agatcatgaa
     8521 tgaggggtgg gcaagttttg cacatacccg catcatgacc gagcgcgccc tggagccgga
     8581 cgaactgatc gactacgcgg agcaccattc cggcacggtg gccgtccacc ccggccgcct
     8641 caacccttat aagttgggcc tcgaactata ccgcgacatc gaagaccgct ggaatcgcgg
     8701 cgcgttcggc ccggagtacg aggcatgcga gagcatgcag gctcgccgcg agtgggaccg
     8761 gaaactggga ctgggccggc agaaggtgtt cgaggtgcgc cgtgtctaca acgatatcgg
     8821 cttcatggac gagttcctga ccgaagactt cgcgcgcgag catcagctgt tcacattcga
     8881 ctacaacgaa gacgacgggc aatacgaaat cgccagccgc gagtttcagg agatcaagcg
     8941 ccgcctgctc ttccaactga ccaacttcgg ccagccgatc gtcgagatcg ccgacggcaa
     9001 ctatcagaac cggggcgagc tgtatctcgt ccatcgccac gaagggatcg acctcgacgt
     9061 tccgttcgcg caggacacgc tccgcaatct gtacacgctc tggggccggc cggtccacgt
     9121 cgagacgcag gtcgaagacg agggccgggt gctgttcagt cacgggccgg acggcggcgc
     9181 acggcgcaat ctgaactgat ccatgctaca atggcgttcg tgtctcaact cgaccccgaa
     9241 cgccagcagc aagccaaaat ctacgcccgc atccgccgcc ggctgttcct ggtcgacgtc
     9301 ctcatcggcg gcgcgtatgc gctggcctgg ctgatcctcg gctggtctac ttcactgaag
     9361 gcgtcactcc tcacagtgac aactgataac tggctactgg tcgccctatt cggcgctgtc
     9421 ttcggcggca tctactacct catcaacctt ccgctctcgt acacctctgg cttcgtcctg
     9481 ccgcatcgat tcggtctttc ggcgcaaacg cgcaagggtt ggatcggcga tcaggtcaag
     9541 atcctctcgc tgggcgcgat tctcggcggg ctgatcctcg aagtcgtgta cgcagtgctg
     9601 cgcgccgcgc cggagacgtg gtggctgtgg gcggccgggc tgctgctgct gttcaacgtc
     9661 gtcctggcca acctggcgcc gatcctcatc gcgccgctgt tcttcaagtt tgtcccgctc
     9721 ggcgaagaac acgccgatct cgcccagcgc ctgatccggc tggcggggcg cgcgaacacg
     9781 aaggtgcgcg gcgtgttcaa gttcgacatg agccgccgca cgaaagcggc taacgctgcg
     9841 ctgatgggac tcggcaacac gcgccgcatc gtcctcggcg atacgctcat cgaaggattc
     9901 acgcccgacg agatcgagac cgtcctggcg cacgagcttg ggcatcacgt caacaaagac
     9961 atcccgctcg gcatccttgt cgagacggcg ctcaccgtcg gcgggctgta cctggcttcg
    10021 cttgcgctca attggggcgt ggcggcgttc gggttcggag gcccggccga cgtcgccgcg
    10081 ctgccgctct tcgcgttggt gatgggcgcc tacgggctga tcacaatgcc gctgggcaat
    10141 gcctattcgc gttggcgcga gcgccgcgcc gacgagtacg cgctgcgcgc gaccggcaac
    10201 ggcccggcgt acgcctccgc gctcacccgc ctggccaatc agaacctggc cgaagtcgat
    10261 cccgagccgt gggtggagtt ccttttctat tcacatccag cgctcggcaa gcgcatcgcg
    10321 atggcccggg cgagcggtga gtgaaggtcg tccctggaac gaactgaaca cggcgatcat
    10381 ggcctgcgag aaatgtccgc gcctgagagc gtactgcgcg aacatcgccc aggagaaacg
    10441 ccgcgccttc cgcgatcaaa cctactgggg taggcctgtc cccggctggg gcgacccgaa
    10501 tgcgcgcatc ctgatcgtcg ggttggcgcc tgcggcgcac ggcgcaaacc gcaccggccg
    10561 gatgttcacc ggcgatcgat ccggcagttt cctctacagt gcgctgtatc gcaccggcct
    10621 ggccaatcag ccgacgtctg tgtcgagaga cgatggcttg aagctgaccg acgtgtacat
    10681 cacggcagcc acacgctgcg ccccgcccga caacaagccc acaccgggtg agctgcacaa
    10741 ctgccggcct tacctggttg aagagctgcg cctgctccag agggcacgcg tgatcgtccc
    10801 gttggggagg atcgcgttcg acaactccct ccgcgcgctc gaagcggtcg gcgcttcggt
    10861 gctgtcgcct cggccgagat tcgcccacgg cgcaacgtgc cgcgtcgaaa cctgccacgt
    10921 tcacaccctc atcgcttcgt atcaccccag ccggcgcaac acgcagaccg gactgctcac
    10981 gcccgtcatg ttcgatcgca tcttccagca cgctcgcgcg ttggcgcgct gacacatcca
    11041 ctggcgcctc cgttctggtg atgacatccg cccgtgatac aattggatgc atgcgcctct
    11101 tccctttgct ctgcgccatc gcgctgttcg ccgcgcccat cgcggcttcg gctcagtccg
    11161 acgccgtgtc ggatctgctg aaccgcatca acgcgctacg ccgccagaac gggctggctc
    11221 ctctggagct gaacgatgcg ctcaacgcag cggcccagcg tcacagcctg gacatggcga
    11281 ataccggcaa cgtcgaccac accggctcgg atggatcgac acccgagcag cgcatcgacg
    11341 cggcgggcta cgcgccgcgc tacacctggg gtgagaacat ctacggcggc gggatcgcga
    11401 cggtcgacga cgcatgggcc ttctggacga gctcgacagt gcatcggaac aacctgctca
    11461 acgagcgcta ccgtgaggtc gggatcggcg cggcggcggg cgcgaacgga acgtactaca
    11521 cgctgacgtt cggcgcacga ccgggcgtgc taccgttttt cgtcgaccag ggcagcgtgg
    11581 tggacagccc cgccgtcacg ctgacgctga gcaacgagga agctgagccg gtcggctcgg
    11641 gcggaaccat gggacgcgcc gtcgaaatcc gcgttggcga aggagaagtc ctgagcgccg
    11701 tcgcctggca gccctggcag cgcacgattc cgttcacgct gtcgagcgtc gacggcgagc
    11761 accgcatccg cgtcgaatat cgcgacgcga acggcgtcgt cgcctcttac ttccggctcg
    11821 tgacgctgac tggcgcggct gccccatcca cggcgacccc gacgaccatt ccgaccgtcc
    11881 agccggcgag cacgcacacg gcgac
//
LOCUS       DBNHXB010000183         3895 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3732538, whole genome shotgun sequence.
ACCESSION   DBNHXB010000183 DBNHXB010000000
VERSION     DBNHXB010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3732538"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="AC162G_07355"
     CDS             complement(<1..518)
                     /locus_tag="AC162G_07355"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR5075664.1"
                     /translation="MITLRHFTHRYPQSDRPALADVDLHIPEGAFALVAGPSGAGKST
                     LLRALNGLTPHFSGGQSAGTISVAGRDPIADGPAILSRSVGFVFQDPEAQFVVDQVED
                     EIAFALENAGLPVSLMRERIEDALSLLGLTGLRRRQVGRLSGGEMQRVAIASALALRT
                     RVLALDEPTSQL"
     gene            complement(577..2646)
                     /locus_tag="AC162G_07360"
     CDS             complement(577..2646)
                     /locus_tag="AC162G_07360"
                     /inference="COORDINATES: protein motif:HMM:NF014420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR5075665.1"
                     /translation="MASVDEVVREPRSSALRLNGLILSRSASVAIYLLTAAIGAMAFA
                     APFVDAAQSGGAALSTSPVWLTLLTGLCLAALIVEMQGRAMNAKTVALLGVLVAVNSV
                     LRFIETALPGPGGFSPIFVPVILSGFVFGVRFGFLMGAFTLLASALITAGVGPWLPYQ
                     MLVTGWIGMSAGLLGHWAGSRRNDAKRRRLWLGMLIVFGFGWGFLYGAIINLWFWPLA
                     VGPAEQHWQPGIGVGAALERYAVYYAATSAWWDFFSAAGNALLIALVGLPTLRALERF
                     RLRMSQPTSTLLDADLRSGAEGTPRPAGASTPGDSVGHETVWRRPEAEPPSARTAPSD
                     ARRVMVERGLHPLTWLTWLSAALVVVMSTRNPMYLIEVLLIAGIVSVWTNAGERSREV
                     WRPTPRFALFVILIGALINALSSHFGATVLFSLPDGLPLIGGPVTLEAVVYGAMAGLA
                     LLVMLALFAVFNQSLSAHQLVRLTPRAFHGAGVTLSIALTYVPATRRSLNQIREAQAV
                     RGHQLRSWRDWLPLWMPLLVSGLERAFQLAEAMVARGFGAASDRPALPWLRAGLIGGL
                     IVLLAGEVLSAFLPNARALSDPLLIAGAAAFAIFIWLAGRAVPYTPYRSHPWRIGDAL
                     VVAGAALSFATIVWPGLGASCGEILGCRTTVAFTPYPTLTLPGFDPLVGLALLGLLLP
                     AWVTNRL"
     gene            complement(2802..>3895)
                     /locus_tag="AC162G_07365"
     CDS             complement(2802..>3895)
                     /locus_tag="AC162G_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075666.1"
                     /translation="SDLNQAWAILGLSAANRPVPISATNYLIDSQAPDGTWFFGDADA
                     TAYAVVALVESGNVEPADPVIQKAIEFFRASQTPEGGWRPSWDTDPINADTTGWIVQA
                     LTVAGDAPMAESWAKPGGNPLTALIGLQKPDGSIGGTYVNAYSTIEALFGLAGKPLFS
                     AGPANRAGLVVQLGTGSTLTRCVEFGEDSLSGFDILQRSGLSVEASFDAGTGTFVCRI
                     ENAGCPVENCSCAFPPDYWSYWHLRDGSWAYASVGASSYTVHDGDVEGWSWGAGEPPA
                     LFTFDEICAPSSAAEVAPAPTVAALPPATPQAVAATPEPATESPSASGQPAQEQTSDS
                     PSPAGYVVFAVIAVVLGGILLLPRIRRRL"
BASE COUNT          788 a         1287 c         1261 g          559 t
ORIGIN      
        1 tcgagctggc tggtcggctc gtcgagcgcc aaaacacgcg tccgcagcgc cagcgccgag
       61 gcgatcgcga cgcgctgcat ctcgccgccc gacaatcggc ctacctggcg gcggcgcagg
      121 ccggtcaatc ccaataacga cagcgcatct tcgatccgtt cgcgcatcaa cgagacgggt
      181 aacccggcgt tctccagcgc gaaggcgatc tcgtcctcaa cctgatcgac gacgaactgg
      241 gcttccggat cctggaaaac gaaaccgacg ctgcggctca agatagccgg gccatcggcg
      301 atcgggtcgc gcccggcgac agagattgtt ccggccgatt gtcctccgct gaaatgcggc
      361 gtcagcccgt tgagcgcgcg cagcagcgtc gacttgcccg cgccggatgg gcctgcgacc
      421 agcgcgaacg cgccttcggg aatgtgcagg tcgacgtctg ccagagcagg ccgatccgat
      481 tgcggataac gatgcgtgaa atgcctgagc gtgatcattg gaacttcaaa acatcaaact
      541 tcaaaacttc caacctccaa cttccaacct caaacctcaa agtctattcg tcacccacgc
      601 gggcagcagc aagccgagta gcgccagccc gacgagcggg tcgaagccgg gcagcgtcaa
      661 cgtcggatac ggggtaaagg cgaccgtggt gcggcatccc agaatctcgc cgcacgatgc
      721 gcccagtcca ggccatacga tggtagcgaa cgacagagca gcgccggcga ccacgagggc
      781 gtctccaatg cgccacggat gcgagcggta cggcgtatag ggcacagccc ggccggcaag
      841 ccagatgaag atcgcgaacg cggcggcgcc ggcgatgagt aacggatccg acagcgcgcg
      901 cgcgttcggg aggaaagcgc tcaagacttc accggccaac aggacgatca gcccgccgat
      961 caggccggcg cgcagccagg gcaaagccgg tcgatccgag gcagcgccga acccacgcgc
     1021 caccatcgct tcggccaact gaaacgctcg ctccagcccg ctgacgagca gaggcatcca
     1081 cagcgggagc cagtcacgcc acgagcgaag ctggtggccg cgcacggcct gcgcttcgcg
     1141 tatctggttg agtgaccggc gcgtggcagg cacgtaggtc agcgcgatcg acagcgtgac
     1201 gcccgccccg tgaaacgcgc ggggcgtcag ccgaaccaac tggtgcgccg acagggactg
     1261 attgaacacc gcgaagagag cgagcatgac cagaagcgcc agtcccgcca tcgcgccgta
     1321 gacgacggcc tccagtgtca ccggcccgcc gatcagcggc agcccgtcag gcagactgaa
     1381 caacaccgtc gcgccgaagt ggctgctcag cgcgttgatc agcgcgccga tcaggatcac
     1441 aaacagcgcg aaacgcggcg tgggtctcca cacttcgcgg ctgcgctcgc cggcgttcgt
     1501 ccagacggag acgatccccg caatcaggag cacttcaatc agatacatcg gattgcgcgt
     1561 cgacatgacg acgaccaggg ctgcgctcag ccaggtcagc caggtcagtg ggtgaaggcc
     1621 gcgttcaacc ataacccggc gcgcgtcgga tggagcggtg cgtgcgcttg gcggctcggc
     1681 ctcgggtcgg cgccatacgg tttcatgtcc gacggagtca ccgggcgtgc tcgcccccgc
     1741 tgggcggggc gtcccctccg ctccgctacg aagatctgcg tcaagcagcg tcgacgtggg
     1801 ttgactcatg cgcaggcgga atcgctcgag cgcgcgaagt gtcggcagtc ccaccagcgc
     1861 gatcagcaag gcattgcctg ctgcgctgaa gaagtcccac caggcggatg tggcggcgta
     1921 gtagactgca taacgctcca gggcggcgcc gacgccgatg ccgggctgcc agtgctgctc
     1981 tgcagggccg acggccaacg gccagaacca gagattgatg atcgcgccgt agaggaatcc
     2041 ccatccgaag ccgaagacga tcaacattcc cagccacaaa cggcgacgct tcgcgtcgtt
     2101 gcgccgcgag cctgcccaat ggcccaacag tccggcgctc atgccgatcc agcccgtcac
     2161 gagcatctgg tagggaagcc acgggccgac gcctgccgtg atcagcgccg aggccagcag
     2221 cgtgaacgcg cccatcagga atccgaagcg cacgccgaag acgaatccgc tcaggatgac
     2281 cggcacgaag atggggctga agccacccgg gccgggcagc gccgtctcga tgaaacggag
     2341 taccgagttg acggcgacga ggacgccaag cagcgcgacg gtcttggcat tcatcgcgcg
     2401 gccctgcatc tcgacgatga gcgcggccag gcacaacccg gtgagcagcg tgagccacac
     2461 gggagaggtg ctcagtgcag ctccgccgga ttgtgccgcg tccacaaacg gagcggcaaa
     2521 cgccatcgcg ccgattgccg ccgtcaacag gtagatggcg acgctggcgg aacgggagag
     2581 gataagccca ttcagccgaa gagctgacga tcgaggttcg cgtacaactt cgtcaactga
     2641 tgccatagcg cagccccgcc gcaaccaaaa gactaaccac agatgacacg gattacacag
     2701 atatgttgag ggttagccat ccgtggtacc gaggagcgag cacgcccacg tgcgagcggc
     2761 atccagggat gccttgctcc gctcatttga atcgtgttgt tctatagccg cctgcgaatg
     2821 cgtggcagca gaagtatccc tcccaacacc accgcgataa ccgcgaatac gacatagccc
     2881 gccggggaag gtgaatcgga tgtctgctcc tgcgccggct ggccggatgc gctcggcgac
     2941 tccgtcgccg gctcgggggt cgccgcgacg gcctgcggcg tcgccggcgg caacgctgcc
     3001 acagtgggcg cgggcgcgac ctcggcggcg cttgatggcg cacagatttc atcaaaggtg
     3061 aagagtgccg gtggttctcc agcgccccag ctccagccct cgacatcgcc gtcgtgaacc
     3121 gtgtaggagc tggccccaac cgaagcgtag gcccaacttc catccctcaa atgccagtaa
     3181 gaccagtagt cgggcgggaa ggcgcacgag cagttctcga ccggacagcc ggcattctcg
     3241 atccggcaca cgaaggtccc cgtgcccgca tcgaacgagg cttccacgga taggccggat
     3301 cgctgcaaga tgtcgaaccc tgacaacgaa tcttcgccga actcgacgca gcgggttaag
     3361 gtcgagccgg tgccaagctg cacgaccagc ccggcacggt tcgccggccc agcagagaac
     3421 agcggcttcc cggcgaggcc gaagagcgcc tcgatcgtgc tgtaggcgtt gacatacgtg
     3481 ccgccgatgc tgccatcggg tttctgcaga ccgatcagag ccgtaagggg attgccgccc
     3541 ggtttcgccc acgactcggc cataggcgcg tcgccggcga cggtcagcgc ctgcacgatc
     3601 cagccggtgg tgtcggcgtt gatcgggtca gtatcccaag acggccgcca accgccttcg
     3661 ggcgtctggc tggcgcgaaa gaattcgatc gctttctgga tgaccggatc cgccggctcg
     3721 acgttgccgc tctcgacgag cgcgacgacg gcgtaggctg tcgcatccgc atcgccgaag
     3781 aaccaggtgc cgtccggcgc ctgagagtcg atcaggtagt tcgtcgccga gatgggaacc
     3841 ggacggttgg cggcgctcaa tccgaggatg gcccaggcct ggttgagatc gctcg
//
LOCUS       DBNHXB010000184         5414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3732735, whole genome shotgun sequence.
ACCESSION   DBNHXB010000184 DBNHXB010000000
VERSION     DBNHXB010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3732735"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..347)
                     /locus_tag="AC162G_07370"
     CDS             complement(201..347)
                     /locus_tag="AC162G_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HIR5075667.1"
                     /translation="MLFAPREEGQGLVEYALILVLVAIVVIVILAVLGPAIGNIFSEI
                     VNAV"
     gene            576..1826
                     /locus_tag="AC162G_07375"
     CDS             576..1826
                     /locus_tag="AC162G_07375"
                     /inference="COORDINATES: protein motif:HMM:NF018613.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGDG synthase family glycosyltransferase"
                     /protein_id="HIR5075668.1"
                     /translation="MNKPLRIMLLMAKTGGGHGAVADAIAQTLKQLYGDAVEPRVADG
                     LRDFAPFPFVLIETTYPLQVKLGGDGYGMAWRALNDAVRAKRLMKSTWPFVRSAVLDI
                     VAQPVDAIVAVHPLFVYPCLWAMHRTGRRLPFITMVSDLIVVHALWCDPETDSMLVPT
                     TTSRGYAIQHGVPAQKIRVTGLPIRPQFAEPIEPRPAARARLGLQADKRAVLLMGGGD
                     GLGNLGEIVRAIGFSGLDVQMIVVAGRNEKLRKQFAETEWPIPTRIYGFTPEIPALMN
                     AADVLITKAGPTTVAEALARELPTILSGFIPTQEEKNVTYLVDAGAGVLAEEPDRIVG
                     VLRSWLSDDGQTLARMSAAARAAARPRAAFDAAEAIYTTARDTPVIVQPPRREPLLAA
                     LERFLGADGVVDYLKEGVYNRRRR"
     gene            2106..3728
                     /locus_tag="AC162G_07380"
     CDS             2106..3728
                     /locus_tag="AC162G_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR5075669.1"
                     /translation="MISVNLVDVSLNLAGRPILAGLSLEIHADRVTGLVGPNGVGKSS
                     LLKLIAGEYPPDAGTLTRARGVTIGYLAQEPVLAPDRTALAEAMTAVPELARIEAGLE
                     RIEARLSDPTVYGDAKKLARALDQQTELHARYDALGGATFERRVRDTLAGLGFDEADL
                     HAHTTVLSGGQKKLVGLARLLVAQPDLLLLDEPDNHLDLNGKIFLEKFIQSYPGAVVI
                     VSHDRYLLDRVVDEIADLEGGRIQVYPGGYSEFAFEKEMRLARQQHLYQVQQREIGRL
                     ETAARRLLVWGRTYDNPKLIRRGKNILTRIEKMDKIDRPVLERKRIGLELDGWRGSNK
                     VLEIKRLSKAFGANAVLRDVSLLIRHGERVALVGPNAAGKSLLLRTILEQETPDAGEI
                     VVGPSVQIGYYAQHHETLDFSQTLIDTVRRAKPMSEEAAVAFLLKFLYAYNQTRQLVA
                     DLSGGERSRLQLALLMLSGANFLLLDEPTNNLDIASAEVLESALDDFEGTVLTVSHDR
                     YFVDRFAARLVELRDGVITEYPGTYVEYLGSQ"
     gene            3829..4290
                     /locus_tag="AC162G_07385"
     CDS             3829..4290
                     /locus_tag="AC162G_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR5075670.1"
                     /translation="MEITRQSDYAVRAVLDLAMLSDDERAFSEAIADRQDLPTAFLTK
                     IFSRLSAEGIVTTRRGVKGGVHLARPASEITMLEVIEAIDGPIALNRCTRRPSDCSLD
                     ATCAVYPIWRTLCDELRARLNSITFEMLAASAQSKRAEAASAVLRNLANPT"
     gene            complement(4351..5256)
                     /gene="lipA"
                     /locus_tag="AC162G_07390"
     CDS             complement(4351..5256)
                     /gene="lipA"
                     /locus_tag="AC162G_07390"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR5075671.1"
                     /translation="MEPKHDRSVEVSIRPQRRPDWLKVRAPAGETYEHLKRLMRVKTL
                     HTVCEEALCPNIGECWGRGTATFLMMGDVCTRSCGFCDVKTGRPAPLDWDEPRRVAEA
                     VRAMSLHHVVITSVNRDERKDGGAPIFALVIHEVRQAQPGCTIEVLIPDFKGSLDALK
                     TVVDEQPDILNHNVETVPRLFRKVQPQDRYEWAMTTLGNAKKLNPAQITKSGIMVGLG
                     ETDEEVIEVMRDLVALGVDILTVGQYLQPSKKHLPVERFVTPQQFAEYKRLGLEMGFR
                     WVESGPLVRSSYNADRQARELKVAL"
BASE COUNT          979 a         1700 c         1692 g         1043 t
ORIGIN      
        1 gaccgcggcg attacacgtt tgcgaaggtc ttgggagtaa ggtttcatgc ccaccagtct
       61 aaccgattca cggcgtaaat caaatcggaa accgatctag tctcgcgaag gccgcgctcg
      121 ttacactcac cgggttccaa agcaaagccc gcctccggtg cttcggaggc gggctttggc
      181 gtggttgtga agcggcggga ctagacggcg ttgacgattt ccgagaagat gttgccgatg
      241 gccggaccga ggaccgccag gatgacgatc accacgatgg ccaccagaac caggatcagc
      301 gcgtactcca ccaatccctg tccctcttca cgcggcgcga acaacatggt tatatctcct
      361 ttctaatgaa tgtggggctt ttgcccgtgg ctcggtatcc cacaccgaca gccacatgat
      421 aaaccagatt ggccgatttg tacacaacag tatggttaga atcgacagac ggcgagtggt
      481 accgtggtgc taatcggcca ggcgttctgg tcggtttgac atggtataat cgctgcgccc
      541 cacgggagtg cagctcgatc catccgaggt taacaatgaa caaaccgctg cgtatcatgc
      601 tgctcatggc gaagaccggc ggtggtcatg gcgcggtcgc cgacgccatc gcgcaaacgc
      661 tcaagcaact atacggagac gccgtcgagc cgcgagtcgc tgacggtctg cgcgactttg
      721 cacccttccc attcgtactc atcgaaacga cgtatcccct ccaggtcaag ctgggcggcg
      781 atggctacgg catggcctgg cgggcgctga acgacgccgt ccgcgccaag cgcctcatga
      841 agtccacctg gccgttcgtg cgctcggctg tgctggacat cgtcgcgcag ccggtcgatg
      901 cgattgtggc cgtccatccc ctgttcgtct acccatgcct gtgggccatg catcgcactg
      961 gccggcgcct acccttcatc accatggtat ccgacctgat cgtcgtacac gccctgtggt
     1021 gcgatccgga aacagacagc atgctcgttc ccaccacaac cagccggggc tacgcgatcc
     1081 agcacggcgt tcctgcccag aagatacggg tgaccggcct gccgattcgg ccgcagtttg
     1141 ccgaaccgat cgagccgcgc ccggccgcac gcgcccggct gggccttcag gcggacaagc
     1201 gcgccgtgct gctcatgggc ggcggcgacg gtcttggcaa cctgggcgag atcgttcgcg
     1261 ccatcggctt ctcgggactt gacgtgcaga tgatcgtcgt cgccggtcgc aacgagaagc
     1321 ttaggaagca gtttgcagaa acggagtggc cgatcccgac gcgaatctat ggattcacgc
     1381 cggagattcc ggcgctgatg aacgccgccg acgtattgat caccaaggcc ggcccgacga
     1441 ccgtggccga ggcgctggcc cgtgagctgc cgacgatcct cagcggcttc atcccaacgc
     1501 aagaggaaaa gaacgttacg tatctggtcg acgcgggagc cggcgttctg gcggaagagc
     1561 ctgatcgcat cgtcggcgtg cttcgcagtt ggctgtcgga cgacgggcaa acgctggccc
     1621 ggatgtcggc ggctgcacgc gcggcagcgc gtcctcgcgc cgcgttcgac gccgccgaag
     1681 cgatctacac gaccgcgcgt gatacacctg tcatcgttca gccgccgcgc cgcgagcctc
     1741 tgcttgccgc gctggagcgc ttcctcggcg cggatggcgt cgttgattat ctcaaagaag
     1801 gagtgtataa tcggcgccgt cgataagcgc ctatccgaaa actggatcgc aggctttagt
     1861 ctgctttgtc caaagcggac tgaagtccgc actccgggag ttttcggata ggctttcagc
     1921 agcaggtgag ccgggcaatg gagtcttacg tggtctacct gtcctggatg gttcagcgca
     1981 cgcttcgtgg ctgcatcgtc gcacgtcttt gtcgtattgc gcctgatgac cggttgacgt
     2041 aactgcactt cgacgcttgg accgcaggcg caccgcctgc ggtctttttg tttgcggagg
     2101 ccctcgtgat ctcagtcaat ctcgtcgacg tgtcgctgaa cttagcaggc cggccgatcc
     2161 tcgccggcct atcgctcgag attcacgccg accgcgtgac cggcctggtc gggccgaacg
     2221 gcgtgggcaa gtcgtcactg ctgaaactca tcgctggaga atacccgccc gatgccggaa
     2281 cgctgacccg cgccaggggt gtgacgatcg gctacctggc tcaggaaccc gttctcgctc
     2341 ctgacaggac ggcgctcgcg gaagcgatga ccgccgtgcc tgagctggcg cgaatcgaag
     2401 ccgggcttga gcgcatagag gcacgcctct ctgacccgac cgtgtacggc gatgcgaaaa
     2461 aactcgcccg tgcgctcgac caacagaccg agctgcatgc gcggtacgac gcgcttgggg
     2521 gagccacgtt tgagcggcgc gtgcgcgata cgctggccgg cctcggattt gacgaagcgg
     2581 accttcacgc gcacaccacc gtgctgagcg gcgggcagaa gaagctcgtc ggcctggcgc
     2641 ggctgctcgt cgcccagccc gatctgctcc tgctcgacga gccggacaac cacctcgacc
     2701 tgaacggtaa gatctttctc gagaagttca ttcagagcta tccgggtgcg gtcgtcatcg
     2761 tatcgcacga ccgctacctg ctggatcgcg tcgtcgacga gatcgccgat ctggaaggcg
     2821 ggcgcatcca ggtgtatccc ggcggctatt cggagttcgc attcgagaag gaaatgcgac
     2881 tggcgcggca gcagcacctg taccaggtac agcagcgcga gatcgggcgc ctcgaaacgg
     2941 cggccagacg cctgctggta tgggggcgca cttacgacaa tcccaagctg atccggcgcg
     3001 gcaagaatat cctcacgcgc atcgagaaga tggacaagat cgatcgtccc gtgttggagc
     3061 gcaagcgcat cgggctggag cttgatggct ggcgcggcag caacaaggtg ctggagatca
     3121 agcgtctgtc caaagccttc ggcgcgaacg ccgtccttcg cgacgtgagc ctactcatcc
     3181 ggcacggcga gcgcgtcgcg ttggtggggc cgaacgccgc aggcaagtcg ctgctgctgc
     3241 ggacgatcct ggagcaggaa acgcccgacg cgggcgagat cgtcgtcggc ccaagtgtgc
     3301 agatcggtta ctatgcccag catcacgaga cgctagactt ctcgcagacg ctgatcgaca
     3361 ctgtccgccg cgccaaaccg atgagcgagg aagcggccgt agctttcctg ctcaagttcc
     3421 tgtacgcata caatcagaca cgccaacttg tggccgacct gtcgggcggc gagcgcagcc
     3481 ggctgcaact ggcgctgctg atgctgagcg gggcaaactt cctgctgctc gacgagccga
     3541 cgaacaacct cgacatcgct tcggcggagg tgctcgagtc ggcgctcgac gacttcgagg
     3601 gcacggtgct caccgtctcg cacgaccgtt acttcgtcga tcggttcgcg gcccggctgg
     3661 tcgagctgag agacggcgtc atcaccgagt atcctggcac gtacgttgag tacctgggct
     3721 cgcagtgatg tgtatcgctt tcagccgtga tgtctgtcac tgtcccggcc gcttcgactg
     3781 gtatatgctg ctcctgactc ttcttgtccg ctttgtcagg agtgtgctat ggagatcacc
     3841 cgacagtccg attacgctgt tcgcgccgtg ttggacctgg cgatgctttc agacgacgag
     3901 cgtgcattct cggaggcgat cgccgaccgg caggatctcc ctacggcttt cctcaccaag
     3961 attttctcac gcctctcggc cgagggcatt gtgaccacgc gccggggggt gaagggcggt
     4021 gtgcacttgg cgcgcccggc gagcgagatc acgatgctcg aagtcatcga ggctatcgac
     4081 ggcccgatcg cgctcaaccg ctgcactcgc cgtcccagcg attgctcgct cgatgcaacg
     4141 tgcgccgtgt atcctatatg gcgcacgctg tgcgacgagc tgcgggcccg gctgaacagc
     4201 atcaccttcg agatgctggc cgcctcggcc cagtcgaaac gggccgaggc ggcatccgct
     4261 gtgctccgga atttggcgaa tccgacttga gcgacatgga gttgtcggat agtcgctttc
     4321 gcgatgcgga agaggccaga cttgcgaggt ctaaagggcg actttcaact cgcgcgcctg
     4381 ccgatccgca ttatacgacg accggacgag cggcccggac tcgacccagc ggaagcccat
     4441 ttccagcccg aggcgcttgt attcggcgaa ctgctgcgga gtcacgaagc gctcgaccgg
     4501 caggtgcttc ttgctcggct ggagatattg gccgacggtg agaatatcca ctccgagcgc
     4561 gacgaggtca cgcatgacct cgatcacttc ctcgtccgtc tcgcccagac cgaccatgat
     4621 gccgctcttc gtgatctggg cggggttgag cttcttggcg ttgcccagcg tcgtcatcgc
     4681 ccactcgtag cgatcctgcg gctggacctt cctgaacagg cgcggcacgg tttctacgtt
     4741 gtggttgaga atgtctggct gctcgtcgac gacggtcttc agcgcgtcga ggctaccttt
     4801 gaagtcgggg atcaggacct cgatcgtgca gccgggctgc gcctggcgca cttcgtggat
     4861 gacgagggcg aagatcggcg cgccgccgtc tttgcgctcg tcgcggttga ccgaagtgat
     4921 cacgacgtga tgcaggctca tggcccgcac ggcttcggct acacggcgcg gctcatccca
     4981 gtcgagcggc gcgggccggc cggtcttgac gtcacagaag ccgcacgagc gcgtgcacac
     5041 gtcgcccatc atcaggaagg tcgccgtgcc gcgcccccag cattcgccga tgttcgggca
     5101 cagcgcttcc tcgcacacgg tgtgcagcgt cttcacgcgc atgagacgct tgaggtgctc
     5161 gtacgtctcg cccgctggcg cgcgcacttt cagccaatcc ggccggcgtt gtgggcgaat
     5221 agatacctcg acgctgcgat cgtgcttcgg ttccaaggca gtgatgtcgt cagggctaat
     5281 gacggtcaca gtgtcctcga tttctcgcta cttgagccag ttctcaacct gaagaccgcg
     5341 aatgcgtttg aaatgatcct cattgtttgt gaccaccccc aatccatgca ccaaagcgga
     5401 tgctgcgatc agaa
//
LOCUS       DBNHXB010000185        14607 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1048204, whole genome shotgun sequence.
ACCESSION   DBNHXB010000185 DBNHXB010000000
VERSION     DBNHXB010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 14607)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14607)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14607
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1048204"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="AC162G_07395"
     CDS             complement(<1..291)
                     /locus_tag="AC162G_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075672.1"
                     /translation="MLAEEPTQARSTAAHHAESSGASAAKETSHLVEPILRQIESAHI
                     RTIKVEFPDLYGMARAKAVPARQFPHAVEHGVQFAFPTFALDLAGNPASGTGT"
     gene            complement(578..3931)
                     /locus_tag="AC162G_07400"
     CDS             complement(578..3931)
                     /locus_tag="AC162G_07400"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR5075673.1"
                     /translation="MREAVPIIWTKLRPPLLRQSTFPRERLEALMRQCVTRRLTLVCA
                     APGYGKTTLIASGLSGMGLPLVWYSLSRSDRDIITFYSYLAAGFDHEWPGFADVVRSS
                     FAPGASVQTRPAAFVAAGVNHLASAAAGDFLVVLDDFQLVDQVPEIAETLDLLICHAP
                     LQAHFVIATRAAPAISSLPRLRAEGEAVEIAEADLTFSPDEAATLFAQSLHLSLPDPV
                     VAALTERTEGWVLGLLMAGQSMKGRGSPAAGGPLPESAADRRMLFEYLSEEVMRQQPP
                     AMADFLTSSAILSRLEPAECDTALGRSDSAFRLRQVAQHCLFVVRTDDGWLRYHRLFR
                     EFLLQLLAGDAERVEALHRRAAAYFQEKHDFETAISHWLEARDHRQAAALIVSVSAEM
                     LHAGRLDTLGFWLSQIPESGFAEFPELRVCQGQMCEACGQWDQALEYYERAAQAFTAR
                     GDLLGLSDVLQSKGHILNWREGKHAEAERLHREALSYLSDEHWRKRAALLASLARDQL
                     SAANTTAAQTLYREALAIYEAEADRQGQLDTLLNPGSWLFHSMGDFSQALAVLRRAER
                     LASDLNSSRQLAEAYNNISLNLYFLGRYGESLAFAKQALELSRELGDLHNEAFALLNQ
                     ANALESTCGMSYVELYRQCQRALHMEQALGNRRYVIATLVFMMILARRGGDSSEAVQR
                     GQQALALAAERGLRWLTGFVLVQLGAAQIWIDPAEARARLVEALHIFGDGEDQYHLTV
                     AHFWLAVLYQSENNPAYLDHLRECLRLAVGHNYDYYFRGEAQAAIPLLAAALEHDLWP
                     SYVTPILVAFGSRAAASLSSLLSNASPEARRRAHAVLEEMGVSATPPAARASKPSPKQ
                     APVPLLTIRGFGNFSLWCGSRIVEEREWGRRKSKRLLKYIVLSPDHTLSKDAATDMLW
                     GDADPQSANANFYRTLYNLRRVLEPMSPHSHSHYIALEGGLLRLVSECVHSIDVDEFV
                     RGVEAGRRAARSGDPAAGDHLSAAVRLYADDLSTDDLYDDWLQPWRERLRNQYLDALC
                     DLADLAAGAGQLDLAIQYLRQAFRKDNTYEAACLKLMQALARSGQRAEALQHYSACEK
                     ALAELGLPLSDELLAARRDLLAGPMPLPG"
     gene            complement(3993..5075)
                     /locus_tag="AC162G_07405"
     CDS             complement(3993..5075)
                     /locus_tag="AC162G_07405"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR5075674.1"
                     /translation="MKTYNIGVIPGDGIGPEVVREGLRVLRAVADGFAYNFVEYPYSG
                     EYYLKTKELVPPSVIEEWRALDAVFLGAIGHPDAEPGLVERSVIMGLRFGLDLYINLR
                     PIKLYAEHLCPLKDKGPADVEFVVVRENTEDAYAGVGGIFKKGTPDEVAIAEMIFTRK
                     GTERVMRYAFELARERARLRGDGERKPKVTMVDKANAIRAQDLWTRTFAEVGAAYADV
                     EQDHAYVDACCMWMVKNPEWFDVIVTTNLFGDIITDLGAMIQGGMGIAASGNIHPGRH
                     GMFEPIHGSAPKYAGKNVANPLGAVAATAMMLSFLGEKQAAERVESAVTELLISRRIP
                     SLDARSGLSTSQIGDMVTQGVLAAVS"
     gene            complement(5175..5690)
                     /locus_tag="AC162G_07410"
     CDS             complement(5175..5690)
                     /locus_tag="AC162G_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5075675.1"
                     /translation="MNYDEWEKTVPEAIKGDSLWKMAAYRLAVFLGDLGWHDVTRLVQ
                     DRRTIDLSSQLYEALGSMSANLAEGYSRGSGKDRARFYEYALGSARESRDWYFEGRHI
                     LGEIVVQHRLQLLAEILRLLLTMVPQQRGRSLREENIPYRIEADNDGWDTSINEEVLN
                     ALLQNVPLPDL"
     gene            complement(5763..6905)
                     /locus_tag="AC162G_07415"
     CDS             complement(5763..6905)
                     /locus_tag="AC162G_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5075676.1"
                     /translation="MIGFTLTDEQREFRELAHKFAEKTIRPIAGEADEDEEVPWEAIE
                     KAHQAGLITFSLPEKYGGGGVESVLTKAIVDEEIFWGCAGVATIIGGAALCATPILLA
                     GTEEQKEKYITRLCDPGRVTLGAYALTEPSAGSDPASMITSARCDRGKYILNGYKTFI
                     TNAGIADLYVVFATVDPSRGVDGITAFIVEKDWPGVMPGKKEKKMGIRASHTAGIAFE
                     DVEVPAANRLGAEGDGFKIAMRTFDITRSHIAIGAVGIARAAYEYALAYAHERRQFGK
                     PIFAFQSIAFMLADMAMQIDAARLLTWRAAWLYDNGLPCTTEASMAKAFAADVAMKVT
                     TDAVQILGGYGYSREYPVEKWMRDAKIMQIYEGTSQIQRLVISRRL"
     gene            complement(7002..8177)
                     /locus_tag="AC162G_07420"
     CDS             complement(7002..8177)
                     /locus_tag="AC162G_07420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5075677.1"
                     /translation="MRPIADVVIVGGGIMGASAAFQLARRGLKVTLVEKTFLAGGTTG
                     KSSAIVRQHYSNEATARMALHSLRVFQEFAEIVGGECGFTQTGFVVLASADQREGLEA
                     NVALQRGLGIDTRAISTEELREIAPDIADPESVVAAYEPESGYADPSATVNSLGAAAR
                     RHGATIMQDTEVTGVLMDGGRVRGVVTTRGEIGAGAAVNAAGPWAVRVARMAGIDLPI
                     QPCRVQVAFFAAPPDGRPAPLVFADFPNVTYFRPETGGLTLIGSVDPSEANNYADPDR
                     FNERVDLEFVADMGTRLARRFPTMERGESRGGYAAIYDVTPDWHPVIDEAPPGSGFFV
                     AAGHSGHGFKLGPAVGAMVADLVTGQKTEGLDPSLFRFSRFTEGKLVRGRYEYSIAG"
     gene            complement(8174..8710)
                     /locus_tag="AC162G_07425"
     CDS             complement(8174..8710)
                     /locus_tag="AC162G_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR5075678.1"
                     /translation="MNPIEHLLQEHRDIMAQVADLRAAVRDLEARGDAALPDALPAMR
                     RIGHMMEAQLALHARKEDEALFPAIEAIIGAEGGPTTVMRLEHKDIHAQGELLRQTLH
                     QLNEVEHPAIEAGGDKLRSLAARGGSAESLRATAAEIIDLLDMHFGKEEQILFPMSEQ
                     ILDDETLAEVGRRIEAMR"
     gene            complement(8801..10831)
                     /locus_tag="AC162G_07430"
     CDS             complement(8801..10831)
                     /locus_tag="AC162G_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LeuD/DmdB family oxidoreductase small subunit"
                     /protein_id="HIR5075679.1"
                     /translation="MAFESFYQTTIGELLTTQANHYPDRPFVRMPNGESCTYADVERQ
                     TSALAAGLRGLGLRPGDRLAIILPNIPQFVLSIFAAAKAGLIICPVNVRRRKAEALAR
                     LAKTRPAALVTDAHHLAMARELRDDLLELKQVIALEGGEGAIALNDLLNTDAMLPAPI
                     ARPDDPAAILYTLGNSGEPRGAVLTHASLVRNAAGVASTLDCTPADVFLGAVPFSNAF
                     GLTPTILACAVAGAQLAPLPVYHAGDSLALIEHAQVTVHHGTPTMFALELNHPDFRPE
                     RCASLRTGVMSGAPCPPELVTRVRGQMVPDLIVAYGLTEASPSVTMTRLDDGPITATQ
                     TVGRPMEGVSLKVVGAEGETLPDGVEGELCVHGYNVMRGYWDDPAMTAQMLNADGWLH
                     TGDLAVIDPDGPVRIVGRKDDVINRGGFKIYPGAVEMVLRLFPGVKEAAVVGVPDVIF
                     GELSCACVVRAPGADFGADELLAFARERLTDYAVPDRVVFFDALPRRGSGPVQKDILR
                     TRVRIRGKAWKFGKNVDTDAIIPARHCNTADPRELAQHCMEDADPEFVRKMRRGDVIV
                     ADTNFGCGSSREVAPITIKAAGVSAVIAKSFARIFFRNSINIGLPILECAAAVDGILE
                     GDEIEVEPATGTVRNLTRGETYRAEPFPDFLQRIIDRGGLLGYVEERLAAHQ"
     gene            complement(10915..11346)
                     /locus_tag="AC162G_07435"
     CDS             complement(10915..11346)
                     /locus_tag="AC162G_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HIR5075680.1"
                     /translation="MDTTTTDAVARFMSERDLLARHMGIELLELRPGYSRVGMTLKPY
                     MVNGLGTTHGAAIFALADFAFATACNSHGRTAVALSMDVHFLSSPEPQARLQAEGVEV
                     RCGQRTGLYRMTVTDENGNLVAELHGMAYRKNTHFLEERSQ"
     gene            complement(11463..12758)
                     /gene="leuC"
                     /locus_tag="AC162G_07440"
     CDS             complement(11463..12758)
                     /gene="leuC"
                     /locus_tag="AC162G_07440"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR5075681.1"
                     /translation="MGMTLAEKILAAHSGHDEVHPGQFINASVDIVLASELSGIVAIE
                     EFEKIRGARIFDPQKVVFVMDHFTPAKDIKSAEIVKRCRDFARGHGIRFYDVGRAGIQ
                     HVLLPEQGLVSPGDIIAGADSHTCTHGFLGAFGTGIGSTDAAAAMALGEIWLRVPESL
                     KFVYHGKPPRWVGGKDLILHTIKHIGVDGARYQAMEFTGDALDHLGLADRFTMANMAI
                     EAGAKTGLFAADDKTREFVSGAGGRVGPGAYLQSDPDAEYAAVYEFDVEKYEPFVAVP
                     FLPENVKPVSELDGLEIDQVVIGMCTNGNLEDLRAAAEILRGRKIHSRVRAVIIPGSQ
                     KVYLDALREGLIDIFIEAECNVSTSTCGPCLGGHMGVLAAGERCVSTSNRNFRGRMGH
                     ATSEVYLANPYVAAASAVAGRIASPAEVLNDKSSVISDQ"
     gene            complement(12821..13687)
                     /locus_tag="AC162G_07445"
     CDS             complement(12821..13687)
                     /locus_tag="AC162G_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5075682.1"
                     /translation="MLGDSVVVGRPSFVPVNGIRLAYTEWPGAKGPLICLPGISGHKG
                     TFTHIAERLAPEYHVLALDLRGRGDSDKPREGYGFAYHARDVLAFADAMGLETFAVIG
                     HSFGATVGVYMASIRPKRVRAAVLIDGGCDPNDEVLEAMRPMLRRLARTYATIENYLD
                     AMRSLPYFRPWNATLERYLREDVEVLPDGAIRPKASAEALERDLDVHFYYSMCVHFPA
                     IQCPTLFIRPKLGLLGDHAHVLTEREAAAFVSWIPDCRQVDLPDVNHYTMVLQDDPPV
                     IPPIEAFLAEVL"
     gene            complement(13743..13961)
                     /locus_tag="AC162G_07450"
     CDS             complement(13743..13961)
                     /locus_tag="AC162G_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075683.1"
                     /translation="MNIRTIDLVRLIHLGRGRLRPGDALRERSPGDGMPDRVATAPGI
                     GRDRLAKIMDEYYAAWGWYHEGASLPLC"
     gene            complement(13958..>14607)
                     /locus_tag="AC162G_07455"
     CDS             complement(13958..>14607)
                     /locus_tag="AC162G_07455"
                     /inference="COORDINATES: protein motif:HMM:NF018485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HIR5075684.1"
                     /translation="PVRQPEPGGAQLAKPAGLQWGLVYRSRVVWHLGLVYSAFGFAYI
                     IYMTFFTKFLISESGYSQEAAGSLFMTMGWFTLLSGLMWGIVSDAIGRRGTMVMVYLV
                     QAAAIVLFGLRPAPLGLTLSAVLFGLTAWSLPTIMAATCGDVFGPRLAPAALGFVTLF
                     FGLGQAIGPSVSGAMADAAKTFSTGFLLAGFVSILGAVGALMLRMPSARQGVVKQ"
BASE COUNT         2675 a         4807 c         4522 g         2603 t
ORIGIN      
        1 ggtgccggtg ccggaggccg ggttgccggc cagatcgagc gcgaacgtcg gaaaggcaaa
       61 ctgaacgccg tgctcgaccg cgtgtgggaa ctgccgcgcc ggcacagcct tcgcccgcgc
      121 catgccatac agatcgggaa actccacttt gatcgttcga atgtgcgctg actcgatctg
      181 gcgaagaata ggctcgacga ggtgagaagt ctccttggcg gcgctcgcgc cggaggattc
      241 ggcgtggtgc gccgcagtcg aacgggcttg ggtcggctcc tcggcaagca tggcagcacc
      301 tccgttgttg tggtgggcta atgcaactca gtctagcaaa cagttctccc aaatttctcc
      361 ctgtccgccg ggagatggac gagccatttg ccttatgagc gactcagcct gttttcgcac
      421 ccgcccgatt acgagcgttt ggacagcagt tgcactgccg tccgcccccg ccgcagttgg
      481 actgcggcgg gaactgcgaa aagtgcgctc aacgcgccta cggctgacga cgattggccg
      541 gtggttgtgg tctgattcgt gacagagggt acaatgacta accggggaga ggcatcggcc
      601 ctgcgagcaa atctcgccgc gcagcgagca gctcgtctga gagcggcagg ccgagttcgg
      661 ccagcgcttt ttcacaggca ctgtaatgct gcagcgcttc ggcgcgctgt ccactccgcg
      721 ccagagcctg catcaatttc aggcaggccg cctcataggt gttatctttt cgaaacgcct
      781 gccgtaaata ctggatagca agatcgagct ggcccgcccc agccgccaga tcggccagat
      841 cgcacagcgc gtcaaggtac tgattgcgaa gccgctcgcg ccacggctgc agccagtcgt
      901 cgtagagatc gtcggtcgaa agatcatcgg cataaaggcg aacggcggcg gacaaatgat
      961 cccccgcagc cgggtcaccc gaccgcgccg cgcggcgccc tgcttctacc ccgcgcacga
     1021 actcgtccac gtcaatactg tgcacgcact ctgagaccag gcgcagcaag ccgccttcga
     1081 gcgcgatgta atgcgagtgt gaatggggtg acatcggctc cagcacgcgc cgcaggttgt
     1141 atagcgtgcg atagaagttg gcgttcgccg actggggatc ggcgtcgccc cacagcatgt
     1201 cggtcgcagc atcctttgag agagtatgat ctggactgag cacaatatac ttcagcagcc
     1261 gcttactctt gcggcgtccc cactcgcgct cttccacgat acgggagccg caccacaggc
     1321 tgaagttacc gaaaccgcga atcgtcaaaa gcggcacagg cgcttgcttg ggtgagggct
     1381 tcgacgcccg cgccgcgggc ggcgtcgcag atacccccat ctcctcaagc accgcatgag
     1441 cacgtcgccg cgcctctgga ctcgcgtttg agagcagaga actgagcgag gcggcggcgc
     1501 gagagccaaa cgccaccagt atcggcgtga cgtaggaggg ccagaggtcg tgctccaacg
     1561 cagccgccaa cagcgggatg gccgcttgcg cctcgccccg aaagtaatag tcgtaattgt
     1621 gccccaccgc cagccgcagg cattcgcgga ggtgatcgag ataggccgga ttattctcgg
     1681 actggtagag cacggccagc cagaagtgag ccaccgtcaa gtgatactga tcttcaccat
     1741 cgccgaagat gtgcagcgct tccaccagcc gggcgcgcgc ttcggctggg tcgatccaga
     1801 tttgcgccgc gcccagttgg accagaacga aacctgtcag ccaacgcagg ccgcgctcgg
     1861 cagccagcgc cagcgcctgc tgcccgcgct ggaccgcctc ggagctatcg ccgccgcgcc
     1921 gggcgaggat catcataaag accagcgtgg cgatgacata gcgacgattg ccgagcgcct
     1981 gctccatgtg cagtgcgcgc tggcactggc gatagagctc aacatagctc atcccgcacg
     2041 tcgactccaa tgcgttggcc tgattgagga gcgcaaaggc ttcgttatgg agatctccca
     2101 attcgcggct cagctcaaga gcctgcttgg caaatgccag cgactcgcca taccgcccca
     2161 gaaagtacaa attcagcgag atgttattgt aggcttcggc gagctggcgc gagctgttta
     2221 agtcagaggc cagccgctcg gcgcggcgca agacggcgag cgcctgcgaa aagtcaccca
     2281 tgctgtggaa gagccacgag cctgggttga gcagcgtgtc gagttggcct tgccggtcgg
     2341 cctccgcttc gtaaatcgcg agcgcctcgc gatacagcgt ttgcgcggcg gtcgtgttcg
     2401 ctgccgaaag ctggtcgcgc gccaggctgg ccagcagagc ggcgcgcttg cgccagtgct
     2461 cgtcgctcaa gtaactgagc gcctcgcggt gcagccgctc ggcctcggca tgcttgcctt
     2521 cgcgccagtt caggatgtgt cctttgcttt gcaggacgtc gctcaagcct agcaggtcac
     2581 cgcgcgcggt gaaggcctgc gccgcccgct cgtagtattc cagcgcctga tcccactggc
     2641 cgcacgcctc gcacatctgt ccctggcaca cccgcagctc cgggaattcg gcaaacccgc
     2701 tctctggaat ctggctgagc cagaacccca gcgtgtcgag gcggccggcg tgaagcatct
     2761 cggccgacac agagacgatg agcgccgctg cctggcggtg gtcgcgcgcc tccagccagt
     2821 gagagatggc cgtttcgaag tcgtgcttct cttggaagta cgccgccgct cgacgatgca
     2881 gcgcttccac ccgctccgcg tcgccggcga gaagctgcag caagaactcc cggaacaggc
     2941 gatgataacg cagccagccg tcgtcggtgc ggacgacgaa gaggcagtgc tgcgccacct
     3001 ggcgcagccg gaaggccgag tccgagcggc cgagcgccgt gtcacactca gccggttcca
     3061 ggcgcgatag gatggccgaa ctggtgagga aatccgccat ggcgggcggc tgctggcgca
     3121 tcacttcctc ggagagatac tcgaataaca tccggcgatc ggccgccgat tcgggcagag
     3181 ggccgccggc cgccgggctg ccgcgccctt tcatggattg gcccgccatc agcaagccca
     3241 gcacccagcc ttcggtccgc tcggtgagcg ccgccacaac cggatcgggc aggctcaggt
     3301 ggagcgactg cgcgaagagc gtggccgctt cgtccgggct gaatgtcaga tcggcttcgg
     3361 cgatttccac cgcttcgcct tccgcgcgca ggcgaggcag ggacgagatg gcgggcgcgg
     3421 cgcgcgtggc aatgacaaaa tgcgcctgca gcggggcgtg acaaatcaag agatcgagcg
     3481 tctcggcgat ctcgggaact tgatccacca gttgaaaatc gtcaaggacg acgagaaaat
     3541 cgccggcagc ggcagaggca agatggttga caccggcggc gacaaacgcc gccggccggg
     3601 tctgcacgct cgcgccggga gcgaatgacg accgcaccac gtcggcaaag ccgggccatt
     3661 cgtggtcgaa gccggcggcg aggtaggagt agaacgtgat aatgtcgcga tcgctccggc
     3721 tgaggctgta ccagacgagc ggcaggccca tcccggacaa cccactggcg atgagcgtgg
     3781 tcttgccgta gccgggcgca gcgcacacga gcgtgagccg tcgcgtcacg cattggcgca
     3841 tgagcgcttc cagccgctcg cgcgggaacg tactctggcg caggagcggc ggacgaagtt
     3901 tagtccatat gataggaaca gcttccctca tcggcttatc cagacgcgct ctctaccatc
     3961 cgccatctgc catccgccat caaccatcag cctcagctaa ccgctgccag caccccttgc
     4021 gtgaccatgt cgccaatctg gctcgtgctc aggccgctcc tggcatccag cgaagggatt
     4081 cggcgcgaga ttaacagctc ggtgaccgca ctctcgactc tctcggccgc ctgtttctcg
     4141 cccaggaagc tcaacatcat ggctgtcgcg gccactgcgc ccaatggatt ggcgacgttc
     4201 ttcccggcgt atttcggcgc ggagccgtgg atcggctcga acatgccgtg cctgcccgga
     4261 tggatgttgc ccgacgcagc gatgcccatg ccgccctgga tcatcgcgcc gagatcggtg
     4321 atgatgtcgc cgaacaggtt cgtcgtcacg atcacgtcga accactccgg attcttcacc
     4381 atccacatgc agcacgcatc gacgtaggcg tgatcctgct cgacatcggc gtacgcggcg
     4441 cccacttcgg cgaacgtccg cgtccagagg tcctgcgcgc gtatggcgtt ggctttgtcc
     4501 accatcgtga ccttcggctt ccgctcgccg tcgccccgca gccgcgcgcg ctctcgcgcc
     4561 agctcgaaag cgtagcgcat gacgcgctcg gtgcccttgc gcgtgaagat catctcggcg
     4621 atggcgacct cgtccggcgt gcctttcttg aaaatgccgc ctactcccgc gtaggcgtcc
     4681 tccgtgttct cgcgcaccac cacgaactcc acgtcggccg ggcctttgtc tttgagcggg
     4741 cacaagtgct cggcgtaaag cttgatgggg cgcaggttga tgtacaggtc aagcccgaag
     4801 cgcaggccca tgatgaccga gcgctcgacc aggccgggtt cggcgtcggg atggccgatc
     4861 gcgccgagga agaccgcgtc gagcgcgcgc cattcttcga tcacgctcgg agggacaagc
     4921 tcctttgtct tcaagtagta ttcgccgctg tatggatact cgacgaagtt gtacgcgaag
     4981 ccatccgcca cggcgcgcag cactcgaagc ccctcgcgca ctacctctgg cccgatgcca
     5041 tcgccgggaa tgacgccgat gttgtaggtt ttcatgacat cctttccaca cgggagatgt
     5101 gaggcataag cgtgaaacgt gacgcgtaaa acgtgatgcg tgaaacgtaa ggcgtaaaac
     5161 gtgaggcatg atggctacag gtcgggcagg ggaacattct gcagcaaagc gttcaaaact
     5221 tcctcgttga tggatgtatc ccagccatcg ttgtcagcct cgatgcggta cggaatgttc
     5281 tcttcgcgga ggctacggcc gcgctgctgg ggaaccatgg tcagcagcaa gcgaaggatc
     5341 tccgcgagaa gctgcaggcg gtgttggacg acgatctcac ccagaatgtg gcggccctcg
     5401 aaataccagt cacggctttc ccgagccgaa cctaaagcgt attcgtagaa gcgtgcccga
     5461 tccttgccgc tgccgcgaga ataaccctca gcaagattgg cactcataga accaagcgcc
     5521 tcgtacaact ggctcgacaa gtcgattgtg cgccggtcct gcacgagcct agtgacatcg
     5581 tgccacccca agtcaccaag aaacacagcc agccggtacg ccgccatttt ccagagggaa
     5641 tcccctttaa tcgcctccgg caccgtcttc tcccactcat cgtaattcac cttgcgcctc
     5701 cttcacgttt tgcgccttac gcttacgttt tacgctttac gtttttcgct ctccccttca
     5761 cctcacagcc ttcgtgagat caccaaccgc tgtatctgcg atgtaccttc gtaaatctgc
     5821 atgattttcg cgtcgcgcat ccacttctcg accgggtatt cgcggctgta gccgtagccg
     5881 ccgagaatct gcacggcgtc cgtggtgact ttcatcgcga cgtcggcggc gaaggccttg
     5941 gccatgctcg cctcggtcgt gcacggcagg ccgttgtcgt acagccatgc cgcgcgccag
     6001 gtcagcaggc gggccgcgtc gatctgcatc gccatatcgg ccagcatgaa ggcgatcgat
     6061 tggaatgcga agatcggctt gccaaactgc cgccgctcgt gcgcgtaggc cagcgcgtac
     6121 tcgtaggcgg cacgggcgat gcctaccgcg ccgatggcaa tgtgcgagcg cgtaatgtcg
     6181 aaggtgcgca tggcgatctt aaagccgtcg ccttctgcgc ccagccggtt ggcggccggc
     6241 acctccacgt cctcgaaggc gatgccggcc gtgtgcgagg cgcgaatgcc catcttcttc
     6301 tctttcttgc ccggcatcac gcccggccag tccttttcca cgatgaacgc cgtaatgccg
     6361 tccacgccgc gcgacggatc gaccgtcgcg aatacgacgt agaggtcggc gatgccagcg
     6421 ttcgtgatga acgtcttgta cccgttgagg atatatttgc cccgatcgca ccgggcgcta
     6481 gtgatcatcg acgccggatc ggagccggcc gacggctcgg tgagcgcgta cgcgccgagc
     6541 gtcaccctgc ccggatcgca cagccgggtg atgtatttct ctttctgctc ctcggtgccc
     6601 gccagcaaga tcggcgtcgc gcacagcgca gcgccgccga tgatcgtcgc gacgcccgcg
     6661 catccccaga agatctcctc gtccacgata gctttggtaa ggacgctctc cacgccgccc
     6721 ccgccatatt tctcggggag agagaacgtg atcaatccgg cctggtgagc tttctcgatt
     6781 gcttcccacg gcacttcctc gtcctcgtcc gcctcgcccg cgatcgggcg aatcgtcttt
     6841 tccgcgaact tgtgggccag ctcgcgaaac tcgcgctgct cgtcggtgag cgtgaagccg
     6901 atcatccgag tcatgcctcc agtaagcgga tagcaggtag cagatggcag atagcagata
     6961 gtagatagtg gatgaccggc agccatcggc caccagccat attaccctgc gatactgtac
     7021 tcgtaacggc cacgcacaag cttgccttcc gtgaagcgcg aaaagcggaa gagcgatgga
     7081 tcaagccctt cggtcttctg gcctgtgacc aggtcggcca ccatcgcgcc gacggcaggg
     7141 ccgagcttga atccgtgccc gctgtgtccg gcggcgacga agaacccgct gccgggcggc
     7201 gcttcgtcga tcacggggtg ccagtcgggc gtcacgtcat agatggcggc gtatccgccg
     7261 cgactctcgc cgcgctccat ggtggggaaa cgccgggcca ggcgtgttcc catgtcggcg
     7321 acgaactcca agtcgacgcg ctcgttgaag cggtccggat cggcgtagtt gttcgcttcg
     7381 gacgggtcga ccgagccgat cagcgtcaat ccgccagtct ccggccggaa gtacgtcacg
     7441 ttcggaaagt ccgcgaagac gagcggtgcc ggccggccat cgggcggcgc cgcgaagaac
     7501 gcgacctgca cgcggcatgg ctggatgggc agatcgatcc cggccatgcg ggcgacgcgc
     7561 actgcccacg gtccggctgc gttgaccgcc gcaccggcgc cgatctcgcc gcgcgtggtc
     7621 acgacgcccc gaactcgccc gccgtccatc agcacgccgg tcacctcggt gtcttgcatg
     7681 attgtcgcgc catgacggcg cgctgccgcg cccaacgagt tcaccgtcgc cgagggatcg
     7741 gcgtagccgc tctccggctc gtatgcggcc acgaccgatt cgggatcggc gatgtcgggc
     7801 gcgatctccc gcaattcctc ggtcgagatg gcacgtgtgt cgatgccgag gccgcgctga
     7861 agcgctacgt tggcttccag gccctcgcgc tggtcggcag aggccaacac cacgaatccg
     7921 gtctgcgtga agccacactc tccgccgacg atctcggcga attcctgaaa gacgcgcagc
     7981 gaatgcagcg ccatgcgcgc cgttgcctcg ttcgagtaat gctggcgaac gatggccgac
     8041 gacttaccgg tcgtgccgcc cgcaaggaag gtcttctcga cgagcgtcac tttcagccca
     8101 cgtcgggcca actggaaggc ggcgctcgcg cccatgatcc caccgccgac gatgacgacg
     8161 tctgcgatcg gtctcatcgc atggcctcga ttcgccggcc gacctcggcc agcgtctcgt
     8221 cgtcgagtat ctgctcgctc atcgggaaca ggatctgctc ttccttgccg aagtgcatgt
     8281 ccagcaggtc gatgatctca gccgcggtgg cgcgcaggct ctccgcgctg ccgccgcggg
     8341 ccgccagcga gcgcagcttg tcgccgcccg cctcgatcgc cggatgctcc acctcgttga
     8401 gctgatggag cgtctggcgt agaagctcgc cctgggcgtg tatgtctttg tgctccaggc
     8461 gcatcacggt cgtcggccca ccctccgcgc cgatgatcgc ctcgatcgcc gggaacagcg
     8521 cctcgtcttc cttcctggca tggagcgcga gctgcgcttc catcatgtgg ccgatgcggc
     8581 gcatcgcagg caacgcgtcc ggcagggccg catcgccgcg cgcctccagg tcgcgcacgg
     8641 cggctcgcaa gtcggcgacc tgcgccataa tgtcacgatg ttcctgcaga agatgttcga
     8701 tagggttcac gagcatatct cccccagggc gatccgatga taagacatcg atcaccgatc
     8761 actgatgact gactactgat gactgattac tgataactga ttactgatga gccgccagcc
     8821 tctcttccac ataccccagc agcccgccgc gatcgatgat gcgctgcagg aagtcgggga
     8881 acggctcggc ccgataggtc tcgccccgtg tcaggttgcg caccgtgccg gtggccggct
     8941 ccacttcgat ctcgtcgcct tccaggatgc cgtcgacggc ggcggcgcac tcgagaatgg
     9001 gcaggccgat gttgatcgag ttgcggaaga agatgcgcgc gaacgatttg gcgatgaccg
     9061 ccgacacgcc ggcggccttg atcgtgatcg gggcaacctc gcgcgatgag ccgcagccaa
     9121 agttggtatc ggcgacgatg acatcgccgc gccgcatctt gcgcacgaac tcgggatcgg
     9181 catcttccat gcagtgctgc gccagctcgc gaggatcggc cgtgttgcaa tgacgggccg
     9241 ggatgatcgc atcggtgtcg acgtttttgc cgaacttcca cgccttgccg cgaatgcgca
     9301 cccgggtgcg caggatgtcc ttctgcaccg ggccgctgcc gcgccggggc agcgcgtcga
     9361 agaacaccac ccgatcgggc acggcatagt ccgtcaaacg ctcccgggcg aaggctagca
     9421 gctcgtcggc gccgaagtcc gcgcccggcg cgcgcacgac gcacgcgcac gacaattcgc
     9481 cgaagatcac atcgggcacg cccacgacgg ccgcctcttt gacgccgggg aacaagcgca
     9541 gcaccatctc cacggcgccg gggtagatct tgaagccgcc tcggttgatc acgtcgtcct
     9601 tgcgacccac gatgcgcacc ggcccgtccg gatcgatgac cgccagatcg ccggtgtgca
     9661 gccagccatc ggcgttgagc atctgcgccg tcatcgccgg atcgtcccag tagcctcgca
     9721 tgacgttgta gccgtgcacg cacagctcgc cttccacacc gtctggtaga gtctctccct
     9781 ctgcgccgac gaccttcagc gatacacctt ccatcggacg ccccacggtc tgggtcgccg
     9841 tgatcgggcc atcgtccagc cgggtcatcg tgaccgaggg cgatgcttcg gtcaggccgt
     9901 aggcaacgat cagatcgggg accatttgcc cgcgcacgcg ggttaccagc tcgggcgggc
     9961 acggcgcgcc tgacatgacg ccggtgcgca ggctggcgca ccgctccggg cgaaagtcag
    10021 gatgattgag ctcgagggcg aacatcgtcg gcgtgccgtg atgcacggtc acctgtgcgt
    10081 gctcgatgag cgcgagcgaa tcgcccgcgt gatacaccgg cagcggcgcg agctgcgcgc
    10141 cggcgacggc gcacgccagg atggttggag tgaggccgaa agcattggaa aacggcaccg
    10201 cgccgaggaa cacgtcggcg ggcgtgcagt cgagcgtcga ggcgacgccg gccgcattgc
    10261 gcacgagcga ggcgtgcgtg aggactgcac cgcgcggctc gcccgaattg cccagtgtgt
    10321 agaggatggc cgccggatcg tcaggccggg caatcggcgc cggcagcata gcgtctgtgt
    10381 taagcagatc gttcagggcg atcgcgcctt cgccgccctc tagcgcaatg acctgtttga
    10441 gctcaagcag atcgtcacgc agctcgcgcg ccatcgccag gtgatgcgca tcggtgacga
    10501 gagccgccgg gcgcgtcttc gccagccgcg ccagcgcttc ggctttgcgg cggcgcacgt
    10561 tgacggggca gataatcaac ccggctttgg ccgcggcgaa gatggacagc acgaactggg
    10621 ggatattcgg caagatgatc gcgagccgat cgccgggccg caggcccagc ccgcgcaggc
    10681 cggcagccag cgccgaagtc tggcgctcga catcggcgta ggtgcagctt tcgccgttcg
    10741 gcatccgaac gaaaggccga tccgggtaat ggttcgcttg cgttgtcaac aactctccga
    10801 tcgtcgtttg gtaaaaggat tcgaacgcca tgatcatgcc tctcctgcct atcgcgtatc
    10861 gcttatggca tatcgtgcca cgcgacggat gggcgatatg cgataagcga tatgctattg
    10921 gctgcgttcc tccaaaaagt gcgtgttctt gcggtaggcc atgccgtgca attcggcgac
    10981 cagattcccg ttctcgtcgg tgaccgtcat gcgatacagg ccggtgcgct ggccgcatcg
    11041 cacttccaca ccttcggcct gaaggcgtgc ctgtggctcg ggcgagctta gaaagtgaac
    11101 gtccatgctc aacgcaacgg ctgtgcgacc gtgcgagttg cacgccgtgg caaacgcgaa
    11161 gtcggccagc gcaaagatcg ccgcaccgtg cgtcgtgccc agcccgttga ccatgtacgg
    11221 cttgagcgtc attccgacgc ggctgtagcc cgggcgcagc tccagcagct cgattcccat
    11281 gtggcgagca agcagatccc gctcggacat gaaacgggct acagcgtcgg tggtggtcgt
    11341 gtccattagt acggtggctc gtgatcagtt atcagtaatc agtgaacagt aatcagtgaa
    11401 cagtaatcag tggacagtag ccagtgaaca gccagcggcc gatgccgatc accgataact
    11461 gatcactgat cactgatcac tgacgacttg tcgttcagca cctcggcagg agaagcgata
    11521 cgaccggcaa ccgccgacgc cgccgcaacg tacgggttcg cgagatagac ctcgctcgtc
    11581 gcgtgcccca tgcggccccg gaagttgcga ttgctggtcg agacgcagcg ctcgccagcc
    11641 gcgagcacgc ccatgtgccc acccaggcac ggcccgcagg tgctcgtgct cacattgcac
    11701 tcggcttcga taaagatatc gatcagtcct tcgcgcagcg cgtcgaggta gaccttttgc
    11761 gagccgggaa tgatgacggc gcgcacgcgc gaatggatct ttcggccgcg caggatctcg
    11821 gccgccgcgc gcaagtcctc gaggttgccg ttggtgcaca tgccgatgac gacctgatcg
    11881 atctccaggc cgtccaattc agagaccggt ttgacgttct ccggcaggaa tggcacggcg
    11941 acgaacggct cgtacttctc aacgtcgaac tcgtacacag cagcgtactc cgcgtccggg
    12001 tcactctgca ggtacgcgcc cgggccgaca cgaccccctg ccccacttac gaactcgcgg
    12061 gtcttatcgt cggcggcgaa caggcccgtc ttcgccccgg cttcgatcgc catgttcgcc
    12121 atcgtgaagc ggtcggccag gccgagatga tcaagcgcgt cgccggtgaa ctccatcgcc
    12181 tgatagcgcg cgccgtctac gccgatgtgt ttgatggtat gcaagatcag atccttgccg
    12241 ccgacccacc tgggcggctt accgtgatac acgaacttga ggctttccgg cactctgagc
    12301 cagatttcgc cgagagccat tgccgccgcc gcgtccgtcg acccgatgcc cgtgccgaac
    12361 gcgccgagga agccgtgtgt gcaggtatgc gagtccgccc cggcgatgat gtcgcccggc
    12421 gaaaccaatc cctgctccgg caggaggacg tgctggatcc cggcacgacc gacgtcgtag
    12481 aagcggatgc cgtgcccgcg cgcgaaatca cggcagcgtt tgacgatctc ggcgctcttg
    12541 atgtctttgg cgggcgtgaa gtgatccatc acgaagacga ccttctgcgg atcgaatatc
    12601 cgcgcgcccc tgatcttttc gaactcctcg atcgccacga tgccggacag ctcggaggcc
    12661 agcacaatgt ccacgctggc gttgatgaac tggccggggt gaacttcgtc gtggcccgaa
    12721 tgcgcggcga ggatcttttc ggcgagagtc attcccatga gtcaatctcc ctccaacctc
    12781 caacttccaa ccttcaacgt ccaacctcca accccctacc tcacaacact tcggccagaa
    12841 atgcctcaat cggcgggatg accggcggat cgtcctgcag taccatcgtg tagtgattca
    12901 catccggtag atccacctgg cggcagtctg gaatccagct cacgaaagcc gccgcctcgc
    12961 gctcggtgag aacgtgcgcg tggtcgccga gcaatcccag cttcgggcgg atgaacagcg
    13021 tcgggcactg tatcgccggg aagtgaacgc acatgctgta atagaaatgg acgtccaggt
    13081 cgcgctcgag cgcttcggcc gaagcttttg gccggatcgc gccatccggc agcacttcga
    13141 cgtcttcgcg caaataacgc tccagtgtcg cgttccacgg ccggaaatac ggcagcgagc
    13201 gcatcgcgtc caggtagttc tctattgtcg cataagttct cgccagccgg cggagcatgg
    13261 ggcgcatcgc ttccaacact tcgtcgtttg gatcgcaccc gccgtcgatc agcacggccg
    13321 cccgcacacg cttgggccgg atcgacgcca tgtacacccc gaccgtcgcg ccaaacgaat
    13381 ggccgatgac ggcgaacgtc tccaggccca tcgcatccgc gaaggcgagc acgtcgcgtg
    13441 catggtaagc aaaaccgtag ccctcgcggg gcttgtcact gtcgccacgg ccgcgcaggt
    13501 cgagcgcgag cacgtgatac tccggcgcga gtcgctcggc gatgtgagtg aaggtccctt
    13561 tgtgcccgga gatgcccggc aggcagatca atgggccttt tgcgccgggc cactccgtgt
    13621 acgccagccg gatgccgttg accggaacga acgaagggcg gccgacgacg actgaatctc
    13681 caagcactat tcgatctcct caacttctgt catgcttcgg cttcgctgat gcttcgcttc
    13741 gctcagcaga gcggcagact ggcgccttcg tgataccacc cccacgccgc gtagtactcg
    13801 tccattattt tggcgagcct gtcgcgcccg ataccgggag ccgtcgccac gcgatcgggc
    13861 atcccatcgc cgggcgaccg ctcgcgcagc gcatcgccgg gacgcaggcg gccgcgtccc
    13921 agatggatca gtcgtacgag atcgatcgtt ctgatattca ttgcttcaca accccctgtc
    13981 tggcggaagg catgcggagc atcagcgcgc ccaccgcacc caggatcgag acgaaccccg
    14041 ccagcagaaa tccggtgctg aatgtcttgg ctgcgtcagc catggctccc gagacgctcg
    14101 gtccgatcgc ctgtcccagg ccgaagaaca gcgtgacgaa tcccagagcg gccggcgcca
    14161 gcctggggcc gaacacgtcg ccacaggtcg cagccatgat ggtgggcagg ctccaggccg
    14221 tgagaccgaa cagaaccgcc gagagcgtaa gcccgagcgg cgcgggccgc aggccgaaca
    14281 gtacgatggc ggcggcctga accaggtata ccatcaccat cgtgcctctc cgcccaatgg
    14341 catccgatac gatgccccac atcagcccgc tcaacagcgt gaaccagccc atcgtcatga
    14401 acagcgatcc ggccgcctcc tggctgtacc cgctctccga gatcaggaac ttggtgaaga
    14461 acgtcatgta gatgatgtaa gcgaacccga aggcgctgta caccagtccc aggtgccaga
    14521 cgacccgcga gcgatacacc agtccccact gaagtccagc cggcttcgcc agttgcgcgc
    14581 cgccgggttc gggctgccga accggat
//
LOCUS       DBNHXB010000186        19674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3061939, whole genome shotgun sequence.
ACCESSION   DBNHXB010000186 DBNHXB010000000
VERSION     DBNHXB010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 19674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19674
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3061939"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..677
                     /locus_tag="AC162G_07460"
     CDS             18..677
                     /locus_tag="AC162G_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR5075685.1"
                     /translation="MKVIIMGCGRVGEELCRIMLGDGHEVAIIDYDQAALDRLGPHFR
                     GRQVLGVGFDRDVLLQAGIEQADAFAATSSSDNANIIAARIARDIFHVPRVVARLFDP
                     RRAEIYRRLGLVTVSSTAWGAERIHELLTHSELDPVMAFGNGEVSLLRIEAPLHLVGR
                     QVRDLSVPGEIGVVAIVRSGQAMLPTLGTELRDGDILYLAVLASALERAESILGVGEG
                     V"
     gene            complement(654..1826)
                     /locus_tag="AC162G_07465"
     CDS             complement(654..1826)
                     /locus_tag="AC162G_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HIR5075686.1"
                     /translation="MKHYDFIMAGGGLAGLSLAYHLIHSSLRESSILIIDKDTKDRND
                     RTWCFWTDRPTPFDDIIFRSWDRLRFVGEGFERVIDLGAYRYQMIRGIDFYRHVRQAL
                     STRANVEFVRGAIDRIEDGADAARVSVGDRVYAGRWVFDSLFRPAEFEAAPARYRHLK
                     QHFRGWVIEAQRATFDPRTPTLFDFRTLQDGALRFMYVLPFSERRALVEHTLFSAELL
                     AREEYEQALREYIEKRLEIGEYRIVEDESGVIPMTDRPFPRCAGRRVLRIGTQGGRVK
                     PSTGYAFLRIQRDSAAMVESLLRRGHPFDVPKDSAWYRLHDSIMLDVMAQQGGQAHSL
                     FTALFRRNPVRRIFRFLDETGSLLNDVALLASLPPWPFLRALLRVYARRLDSFTHA"
     gene            complement(1823..3124)
                     /locus_tag="AC162G_07470"
     CDS             complement(1823..3124)
                     /locus_tag="AC162G_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR5075687.1"
                     /translation="MPHLLNPPEPQGLTGLHALRAMWQRRTILAALEVFHRELGDIFR
                     ITLPGFKPIMLAGPEANRFVLIISRVDLRWRTEGDPVTSLLRRGVLVTDGEEHDALRR
                     RMDPSLHRRMLSGYVEAMWRSADRVTAAWTDNAPRDMLVEMRRIALLILTKTMFGVDF
                     APDLRALWPSILKTLAYISPGPWILWRNLPRPGYTRALKRVDDYLYRIIAARRAQPGS
                     ADDLLGALIASGLSDGLIRDQLLTMLIAGHDTSTALLAWALYLLGAHPDAMARARAEV
                     DAVLGRRMPAIEHAAQLPYLDRVVKETLRLYPPIHLGTRTAAMDLEFNGYRIPAGARV
                     LYSIYLTHRDPRYWPDPDRFDPERFAPESSKARAPYTYVPFGGGPRICIGFAFAEAEV
                     KIVLARLLQQFKLTFTGHPVHPHMGATLEPRPGVMMHARRR"
     gene            complement(3136..4287)
                     /locus_tag="AC162G_07475"
     CDS             complement(3136..4287)
                     /locus_tag="AC162G_07475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5075688.1"
                     /translation="MNDAWLSFQYGLAAFVIGLLLIAVGNLKAWRRMNRYPAPARFPR
                     ASILVPARNEEDNIEGCVRSLLAQDYPDFEVLVLNDDSGDRTRSILAALDGEPRLRVL
                     IGKPLPPDWLGKHWACHQLAQAAAGDLLLFTDADTHHHPQTLRDAVAALHVERADLLS
                     ALPRQIVGSWAERLIVPIIPWSLFALIPLALAHRLRRTSLVVAVGQYMLFRREAYDRS
                     GGHAAVRQNAVDDIALARRIVSQGLRWRLVDGGGRIECRMYRNLGHVMEGLSKNLFAA
                     FDYGWPQFLFVWTWMGLVFVIWPMLLGLGLLGVMLPGFSLEAAVVAAAGSIGLWLIVT
                     RRFSLPAYLALLYPISMALTVIIAFRSMALTLTGRATWRGRTLAVHKSK"
     gene            complement(4284..4889)
                     /locus_tag="AC162G_07480"
     CDS             complement(4284..4889)
                     /locus_tag="AC162G_07480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR5075689.1"
                     /translation="MLTLFWTVVSLLAGAIPFSVVLGRWQARVDIRRFGDANPGAANA
                     WKAGGARIGVPAILLDWAKGALPAGLAHFSASIDGWALVPIALAPIAGHAFSPFLGFK
                     GGKALAVTFGAWSGVTLAEGPITLGLFFTLFLAVQTANAWAVILGMLGFGAHLALRGF
                     DASVLAIWIGNLAILLWKHRRDLREPIRLRGWIVKRMRHTP"
     gene            complement(4889..5833)
                     /locus_tag="AC162G_07485"
     CDS             complement(4889..5833)
                     /locus_tag="AC162G_07485"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HIR5075690.1"
                     /translation="MILQAQTWEHSLLLLAHEAWQSLSIHREPVQNAALLQRAYAHCE
                     AITAEHSRSFHMASQLLPVDKRRAVRALYAFCRTSDDVVDRPSGDAEAMLAAWRERVL
                     MPHPPEDDPVAVAWADTLWRFRIPHRYVEQLIGGVARDLLQQRYETFDDLAAYSYGVA
                     STVGLMSMHITGFSGPEAIPYAIKLGVALQVTNVLRDVGEDYRNGRVYLPQEDLRRFG
                     IAEDQLAEGRVDDRWRAFMQFQIDRNRRLYEDAWPGIAMLNADGRFAIAAAAMLYRAI
                     LDDIEAHDYDVFTRRAHVGTIGRLRRLIDLRLRGLARA"
     gene            5979..7502
                     /locus_tag="AC162G_07490"
     CDS             5979..7502
                     /locus_tag="AC162G_07490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR5075691.1"
                     /translation="MTRRLTALVIGAGIGGIATAARLVRNDFDVTVVEKNAEPGGRCG
                     RLVRDGHRFDIGPTLFLMPEVFAETYAALGERMEDHLDLRRIDPTYRIRFDDDAVLDL
                     TADLNAMQAQLETIEPGSFGGFLRYLAEGNRHYKTSLTHFVGRNFYSPLQYFSLSNLP
                     LLFRLKALVKHYHNVGNYFKHPHLKAAFTFQNMYLGLSPYDAPATYSLLQYTELADGV
                     WFPMRGMYRIIETLVSIAEAKGVRFIYNAPVEKIEVEGKRATGVVLNDGRRLTADIVV
                     ANADLPYVYRELLPDRAETDRLDRMKYTCSAIMFYWGVDKVYPQLGHHNVFLAGDYRA
                     SFDRIFNDDTLPDEPSFYVHAPARTDPSAAPVGHDTLMALVPVGHIDESAPQDWAALQ
                     ARARSVVLQRLARIGVSDLESRLKFEVSYTPREWQSLYNLAKGAAFGLSHNFTQVGYL
                     RPQNRHRRYRNLYFVGASTHPGTGLPIVLLSARLTTERILKEAGLPQPTLVAEPVAV"
     gene            7629..8939
                     /locus_tag="AC162G_07495"
     CDS             7629..8939
                     /locus_tag="AC162G_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR5075692.1"
                     /translation="MPRKEGPVYNLKAVIRETGLKPDTLRAWERRYGMPKPDRTPGKH
                     RLYSQRDIDTLKWLIARQQEGLSISRAVELWRSIAADGQDPLQSSPVASTVKASLSAL
                     AYGDAITDLRAAWVSACTDYDEQRAKSVLTQAFALYPAETVCIHVLQKGLADIGQGWY
                     EGSITVQQEHFTSALALRRLDALIAAMPAPTRSGCILVACPAHEEHTFGPLLITFMLR
                     RQGWDAPFLGANVPLARLESALAATRAKLVVASAQQLHTAANLLDMAHLLRREKVALA
                     FGGLIFNRVPGLRDRIPGYFLGETLEEAPQAVERIMESPLPAPAAAPALPEHRAALVD
                     YRARRAQIEADVLNNIAPEVRQHNYLDVATANLAHEIEAALILGDMEFLDSDIAWIEG
                     LLISHGIPPSVLHYFLDAYRQAVESHMGSVGAPIAAWLATIAPG"
     gene            9017..9163
                     /locus_tag="AC162G_07500"
     CDS             9017..9163
                     /locus_tag="AC162G_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5075693.1"
                     /translation="MRVVITGGTGLIGRAQAASLAADKHEVIVLSRSPQYARSMPGGV
                     KVEK"
     gene            9215..9511
                     /locus_tag="AC162G_07505"
     CDS             9215..9511
                     /locus_tag="AC162G_07505"
                     /inference="COORDINATES: protein motif:HMM:NF019938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HIR5075694.1"
                     /translation="MDNLAGAIRFLVDKPDARGAFNLAAPNSVTNAQLAKILGRVMRR
                     PSYFPVPAFVLRVLLGKKATLVLGGAQIVPKRLIDAGYTFKFPEAEAAFRGLLK"
     gene            9508..9957
                     /locus_tag="AC162G_07510"
     CDS             9508..9957
                     /locus_tag="AC162G_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5075695.1"
                     /translation="MKYRHQFRVRAPLARVAEFHSRTAGLALLIPPPIVARIHRAPII
                     QREGDEVDFTMWLGPLPIRWIARIDSVSETGFVDRQVRGPFERWLHCHTFTPIDASTT
                     AVIDDVQAVLKAHRGWGAVGLTMWLALPLLFAYRGWKTRRALETKPT"
     gene            9954..11621
                     /locus_tag="AC162G_07515"
     CDS             9954..11621
                     /locus_tag="AC162G_07515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR5075696.1"
                     /translation="MSRIVVVGGGVGGLTTAAVLSRAGLDVTVLEAHVYPGGCAGTFY
                     HQGYRFDAGATLAGGFYPGGPMDLVAQAAGIEAWPARSADPAMTVHLPDRAVVVRRSG
                     PPDPVEQREFFGPGADAFWRWQQRTADALWDFALRLPAWPPQSPIDLVRLLSDGAGWA
                     SANLHTLYPDLVLDAFRSVAAHLHHASDRLRVFIDAQLLISAQTTSRYTNALYGASAL
                     DLPRRGVVHLQGGMGAIAHTLADAVRRNGGEVRYRQEATRVVMENGRPVAVETKRGDS
                     FPADVVVFNLPPWNVAGLLGDNAPPRLRALPSHPRDGWGAFVVYVGLDGSVVPGDALL
                     HHQVIVRKSLAEGNTIFLSISPEWDHDRAPMGKRALTISTHTDLDSWWGLFERDRAGY
                     DAHVREYTDRVVAAAAIVLPGLRDAAELILPGTPVTFQRFTRRIGGWVGGFPQTSLLR
                     AWGPRLAPEMWMAGDSIFPGQSTAAVAMGGLRVARSVSGEMGNIAGQDSSDDLVHVAA
                     IDREPRSTVAGTIRRSDVCRFDSRRSRSALDLWRFRRSRMKGKRFRF"
     gene            11636..13108
                     /locus_tag="AC162G_07520"
     CDS             11636..13108
                     /locus_tag="AC162G_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015794820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5075697.1"
                     /translation="MTQVPLVLVTGATGYIGGRLAPRLLEAGYRVRVLARDPARLQGR
                     EWLDRVEVVRGDVLKAETLPSTMAGVGVAYYLVHSMSDTADFHQRDMAAARNFAEAAR
                     AAGVQRIIYLGGLGNPDADLSHHLRSRQETGQALREAGVPVTEFRSAIVVGSGSVSFE
                     MIRYLTERIPLMICPRWVYTRVQPVSIRNVLDYFVAALNTPESAGRIIEIGGADVLTY
                     GQMMTGYAEVRGLRRWLMPVPVLTPRLSSYWVHLVTPIPAAIARPLIQGLRNEVVVRD
                     DSARRLFPAVDLIDYRTAVRHALADLDAGSVETAWSDALVTSQGDAAPVVLATQQGMI
                     MEQRRRIVAAPPDKVYAVFAGLGDRRGWPYANWAWRLRGVIDRLAGGVGLRRGRRDPN
                     DVRAGDAVDFWRVEEVVLERKLRLRAEMKVPGRAWLQFEAQPFSGERTLLLQTAFFAP
                     KGLFGLMYWYLLYPIHSLIFSGMIRRIAQRAEMAAGAERS"
     gene            13105..14517
                     /locus_tag="AC162G_07525"
     CDS             13105..14517
                     /locus_tag="AC162G_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIR5075698.1"
                     /translation="MTTAIWWIRRDLRLSDNQALAEALASTDRIVPAFVFDPCLLASP
                     YVGSKRLAFLLHGLRALDVDLRAKGSRLIVRRGDPAVELAALTRESGAAAIFAEEDIS
                     PYARARDARVAERLPLMQTRGLTAQLPPMVSKPDGKPYTVFTPFSRAWKALPPPQVRD
                     IIAAPKRIETPDDLHSLPIPPHPALPASVPFVSGEAEAQRRLTAFVSSIIGEYADVRN
                     RMDRDGTSQLSPYLRFGMLSARQAIVAAIEAMRSAASAAIHRGAEAWFNELIWREFYI
                     TILHHFPNVRRESFRPELRNIPWENDEGKFAAWCGGRTGYPVVDAAMRQLIENGWMHN
                     RARMIVASFLTKDLLIDWRWGERWFMQHLLDGDPAANNGGWQWTAGTGTDAAPYFRVF
                     NPVLQGAKFDPDGTYVRRWVPELASVPDRFIHKPWDMPHEMQSSIECGIGRDYPAPIV
                     DHEQARERVLAAYREAKAKV"
     gene            14682..15485
                     /locus_tag="AC162G_07530"
     CDS             14682..15485
                     /locus_tag="AC162G_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HIR5075699.1"
                     /translation="MKRFTVVSMVLVLALALTSSAFAETSARVRVVHASPDAPAVDVL
                     VNDAVAFSNAPFKGITQYASLDPATYNVKVVPTGATEPVVIGADLTLEAKDYTVVAVD
                     TLANIEPLILVDNNSAPAAGKAHVRFVHASPDAPAVDIAVAGGGPVLFSNIAFKGVGD
                     YLPVGAGTYDLEVRIAGTDTVALSVPGVQLNNGTVYTVFAMGLAGGTPALQAVPSVDA
                     TPVLLPQSGGESAGNPYLLIVLLGAALLAGGLILRIRPALASARSQNDR"
     gene            15663..16658
                     /locus_tag="AC162G_07535"
     CDS             15663..16658
                     /locus_tag="AC162G_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5075700.1"
                     /translation="MKRFRRIALIIIGLILAVVLVGPFLVPVPPLEGTVPPEQLADSD
                     SRFIDVDGLRVHYKTSGDGSPTFVLLHGFGASVFSWREVMTPLSESGTVIAFDRPAFG
                     LTERPLPGDWDGESPYSPEAQIELTVKLMDQLGVDEAILVGNSAGGTIAMATALEHPD
                     RVLAVVLVDAAIYTGGGTPDWVRPLLGTPQMRRIGPLLARSIQNLGPNLIERAWHDPS
                     KISAEILEGYTKPLKVDNWDVALWELTLASHSLGLDRRVRDIRVPVLVITGDDDRIVP
                     TEQSVRLDGELPDAELVVIPNCGHVPQEECPAEFTQAIVDYLPQLTGSASLSGPQ"
     gene            16735..17493
                     /locus_tag="AC162G_07540"
     CDS             16735..17493
                     /locus_tag="AC162G_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HIR5075701.1"
                     /translation="MNKDSLRQAVNTITFFATVVVNGLANALPLNGQTTAQISDRFPV
                     YFTPANYVFSIWGVIYLLLLGFTLYQALPSQRENPRLRRIGYLPALAGVLNSAWIFLW
                     HYNLFELTVPVMAGLLLTLIAIYLRLDIGRAATTIREKWLVNIPFSVYLGWITIATIA
                     NVTALLYYLNWDGFGISPEVWTVVILLIGAAIASMLIFTRADVAYTLVIVWAYAGIVV
                     KQFGVPIVAGAAVLGALVVIAVLIAARLQRVQPG"
     gene            complement(17541..18776)
                     /locus_tag="AC162G_07545"
     CDS             complement(17541..18776)
                     /locus_tag="AC162G_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5075702.1"
                     /translation="MSGRAAIDLLSQLVDRSLVIVDHQNQAARNHLLETIREYGLGKT
                     MEAGELNLARRRHLDFFVRLAAEHEAYYHAGGEAESLEMEHDNLRAAIEWSLESEDAS
                     TALQLASTMSWLWWMRGYLGERKERLREIVAHVEATAPSGTLERMKLLNAAGALLSTW
                     GDHTEARRLLEEALAIGMELGDKPSSGLALRLLGTAALNQGDYPAARSFLERGVAVWR
                     ELESQWHVGWLLTFLGDTAVHMEDFERAQALYEEAVTWSREFMAKSALAYPVRRLGYL
                     ALYRGDTKEATALCRESLGLNMEVGDKQGMAACLAALAGVAVARGQAVSLRVEDGPPR
                     HAYFRRAAKLFGIIEALLNDVGTRLLQADNIEYERVVAAVRSQLDETAFAAAWAASRS
                     MTLVPAMAYALADDTASWA"
     gene            18786..18947
                     /locus_tag="AC162G_07550"
     CDS             18786..18947
                     /locus_tag="AC162G_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075703.1"
                     /translation="MQHIGHRLGGPPTPEQVAELHKLDKEMSFIGRVDFVLLVVALLT
                     MTTARYWYV"
     gene            19078..19509
                     /locus_tag="AC162G_07555"
     CDS             19078..19509
                     /locus_tag="AC162G_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075704.1"
                     /translation="MTTNDRDERLHPRALPQANWHTLAEFTARATRDSERQAAERVAR
                     MVRELSLPPEFVERIKTAVAHAMLHAIRRDTDQWPGASVGIRILISAAAPPAGSEPRH
                     KNQGWGFFLISRVSDGPQTEPASARPAIELYLYPEGNAREK"
BASE COUNT         3608 a         6176 c         6246 g         3644 t
ORIGIN      
        1 caaggagtcg tcctgatatg aaagtcatca tcatgggctg tggtcgggtg ggcgaagagc
       61 tgtgccggat tatgctcggc gacgggcatg aggttgccat tatcgactac gatcaggcag
      121 cactggatcg ccttggcccg cacttcagag gccgccaagt gctcggcgtc ggcttcgacc
      181 gagacgtgct attgcaggct ggcatcgagc aggcggatgc cttcgccgcg acgagttcat
      241 cagataatgc gaacattatc gcagcacgaa tcgcgcgcga catttttcac gtcccgcgcg
      301 tggtggcccg cctgttcgat ccgcgccgcg cggaaatcta ccggcggctg ggcctggtga
      361 ccgtctcttc gacggcctgg ggcgcggagc gcatccacga gctgttgacg catagcgagc
      421 tcgatccggt gatggcattc ggtaatggtg aagtctcact gctgcggatc gaagcgccgt
      481 tgcacctcgt cgggcggcag gtgcgcgatt tgagtgtgcc aggtgagatc ggcgtcgtcg
      541 ctatcgttcg ctccggtcag gcgatgctgc ccacgctggg cacggagttg cgggatgggg
      601 atatcctcta tttggccgtg ctcgcgtcag ccctggagcg cgccgagagc atattaggcg
      661 tgggtgaagg agtctagtcg ccgcgcatac acgcgcagga gcgctcgcag aaacggccat
      721 ggcggcagcg acgccagaag cgccacatcg ttaagcagcg atccggtctc gtccaagaat
      781 cggaaaatgc ggcgaaccgg gttgcgccgg aagagcgcgg tgaagagcga gtgcgcctgc
      841 ccgccctgct gtgccatcac gtccagcatg atcgaatcgt gcaggcgata ccaagccgaa
      901 tccttcggca cgtcgaacgg atgcccgcgc cgcagcagcg attcgaccat cgccgccgag
      961 tcacgctgaa tccgcaggaa ggcgtagccg gtcgatggct tgacccgccc accttgcgta
     1021 ccgatacgca gcacgcggcg gccggcgcag cgcgggaagg gccgatcggt cattgggatg
     1081 acgccgctct cgtcctcgac gatccgatac tctccgatct ccaatctctt ttcgatgtat
     1141 tcccgtaacg cctgctcgta ctcttcccgc gccagcagtt ccgccgaaaa caacgtgtgc
     1201 tccaccagtg cgcgccgctc ggagaagggc aacacgtaca tgaagcgcag tgcgccgtcc
     1261 tgcagtgtgc gaaagtcgaa cagcgtcggc gtgcgtggat cgaaggttgc gcgctgcgct
     1321 tcgatgaccc agcctcggaa gtgctgtttg agatggcggt agcgcgctgg ggcagcctcg
     1381 aactctgccg gacggaacag gctgtcgaag acccagcgcc ctgcgtaaac ccgatcgccg
     1441 accgacacgc gcgcggcgtc tgcgccgtcc tcgatgcgat cgatcgcgcc ccgaacgaac
     1501 tcgacattag cgcgcgtcga aagcgcctgc cgcacgtggc gatagaagtc gatgccgcga
     1561 atcatctgat agcggtaggc gccgagatca atgactcgct cgaagccctc gccgacgaag
     1621 cgcagccgat cccacgagcg aaaaatgata tcgtcgaaag gagtcggccg atccgtccag
     1681 aagcaccagg ttcgatcgtt acggtctttc gtgtccttgt cgataatgag gattgaactc
     1741 tcgcgcagcg aactgtgaat caggtgatag gcgaggctga gtccggccag cccgccgccg
     1801 gccatgatga aatcgtaatg tttcatcgcc gcctcgcatg catcatcaca cctggacgcg
     1861 gctccagagt cgcgcccatg tgcggatgaa cggggtgccc ggtgaacgtc aatttgaact
     1921 gctgcaacag ccgcgccagc acgatcttca cttcggcctc ggcgaaggcg aatccgatgc
     1981 agatgcgcgg cccgccgccg aatgggacgt aggtgtacgg ggcgcgcgct ttgctgcttt
     2041 ccggcgcgaa tcgctccgga tcaaagcggt cgggatcggg ccagtatcga ggatcgcggt
     2101 gcgtcaggta gatcgagtac agcacgcgcg caccggcggg aatgcgatac ccgttgaact
     2161 ccaaatccat cgccgccgtg cgcgtgccga ggtggatcgg cggatatagg cgtagcgttt
     2221 ccttcaccac tcgatcgaga tacggcaact gcgcggcatg ctcgattgcc ggcatccggc
     2281 ggcccagcac agcgtcgact tcggcgcggg cgcgcgccat cgcgtcggga tgcgcgccca
     2341 gcaggtacag cgcccatgcc agcagcgcgg tgctggtgtc gtggccggcg atcagcatcg
     2401 tcagtagttg atcgcgaatc aggccgtcgc taaggcctga tgcgatcagc gcgccaagca
     2461 agtcgtcggc gctgcccggc tgcgcacggc gcgcagcgat aatgcgatac agatagtcgt
     2521 ccacgcgctt gagcgcgcga gtgtagccag ggcgcggcaa gttgcgccag aggatccatg
     2581 ggccgggcga gatgtacgcc agtgtcttca ggatcgacgg ccatagtgct ctcaggtcgg
     2641 gcgcgaaatc gacgccgaac atcgttttgg tcaggatcag caaggcaatg cggcgcatct
     2701 ccaccagcat gtcgcgcggt gcgttgtccg tccacgcggc ggtgacgcga tcggcgctgc
     2761 gccacatcgc ctcgacgtaa ccggacaaca tgcgccgatg cagcgatggg tccatccgcc
     2821 ggcgcagcgc atcgtgctct tcgccatcgg tgaccagcac gccgcgccgc agcaggctcg
     2881 tcaccggatc gccttccgtg cgccagcgca gatcgacgcg tgagatgatc agcacgaagc
     2941 gattggcttc cggcccggcc agcataattg gcttgaagcc gggcagcgtg atgcggaaga
     3001 tgtcgccgag ttcgcgatga aagacttcca gcgcggccag gatcgtccgg cgctgccaca
     3061 tggcgcgcag cgcgtgaagg ccggtgaggc cttgcggctc aggcggattg agaaggtgag
     3121 gcatcgtttt ggcaatcatt ttgatttgtg caccgcaagc gtacgtcctc gccacgtcgc
     3181 cctgccggtg agcgtgagcg ccatcgatcg gaaggcgatg atcaccgtca gcgccatcga
     3241 gatgggatac agcagtgcca gatacgccgg cagactgaat cgccttgtga cgatcagcca
     3301 cagcccgatc gatccggcag cggccacgac cgcggcttcg agtgagaaac cgggaagcat
     3361 aacgcccagc aggcccagtc ccaacagcat cggccagatc acgaacacga gtcccatcca
     3421 cgtccagacg aacaggaact gcggccagcc gtagtcgaac gccgcaaaca gattcttgct
     3481 caggccttcc atcacgtgac ccagattgcg atacatgcgg cattcaattc gaccgccacc
     3541 gtcgaccaga cgccagcgca agccttgcga cacaatccgc cgcgccagcg cgatgtcatc
     3601 gaccgcattc tgccgcacgg cggcatgtcc gccgctgcga tcgtacgctt cgcgccgaaa
     3661 gagcatgtac tgtccgacgg cgacgacgag cgacgttcgc cgcaagcgat gcgccagagc
     3721 cagcggaatc agcgcaaaca ggctccacgg gatgatcggc acgatcagcc gctcggccca
     3781 cgatccgacg atctgccggg gcagcgcgct cagcagatcg gcgcgttcga cgtgcagcgc
     3841 ggcgacggcg tcgcgcagcg tctgcggatg atggtgtgtg tcggcatcgg tgaacaacag
     3901 cagatcaccg gcggccgcct gcgccagttg atgacaggcc caatgcttgc ccagccagtc
     3961 gggcggcagt ggcttgccga tcagaacgcg caatcgcggt tcgccgtcaa gcgccgccag
     4021 aatcgacctg gtgcgatcgc cggagtcgtc gttcagcacc agcacctcga agtccgggta
     4081 gtcctgcgcc agcagtgagc gcacgcaccc ttcgatgttg tcttcctcgt tccgcgccgg
     4141 caccagaatc gacgcgcgcg ggaagcgcgc cggagcggga taacgattca tccgccgcca
     4201 cgctttcaag ttgcctacgg cgatcagcag cagcccgatg acgaacgccg ccagcccgta
     4261 ttgaaagctc agccatgcat cgttcacggc gtgtgcctca ttcgcttcac gatccacccg
     4321 cgcaggcgga tcggctcgcg cagatcgcgc cgatgtttcc atagcaggat cgccaggttg
     4381 ccgatccaga tcgccagcac ggacgcgtcg aatccacgca acgcgaggtg tgcgccgaag
     4441 cccaacatgc cgagaatgac tgcccaggca ttcgccgtct gaacggcgag gaacagcgtg
     4501 aagaacaggc cgagcgtgat cggtccttcg gcgagtgtga cgccgctcca tgcgccgaac
     4561 gttacagcca gcgccttgcc gcccttgaag ccaagaaacg gggagaaggc gtggccggca
     4621 atcggcgcga gggcgattgg cacgagcgcc cagccgtcga tgctggctga gaagtgagcc
     4681 aatcctgctg gcagagcgcc cttagcccag tccagcaaga ttgctggcac gccaatgcgt
     4741 gcgccgccgg ctttccatgc attcgctgcg ccagggttcg catcgccgaa gcggcgaatg
     4801 tcgacgcgcg cctgccatcg ccctagcaca actgaaaagg gaatggctcc ggccaggagt
     4861 gagacaacag tccagaacag tgtgagcatc aggctcttgc caggccgcgc agccgcaggt
     4921 cgatcaatcg gcgtagcctg cctatcgtgc cgacgtgcgc ccggcgagtg aacacgtcgt
     4981 agtcatgtgc ctcgatatca tcgaggatgg cgcggtacaa catcgccgcc gcggcgatgg
     5041 cgaatcggcc gtcggcgttg agcatggcaa tgcccggcca ggcgtcttcg tacagccggc
     5101 ggttgcggtc gatctggaat tgcatgaacg cccgccagcg gtcgtcgacg cggccttcgg
     5161 ccagttgatc ctccgcaatg ccgaatcgac ggaggtcttc ctgcggcaag tagacgcggc
     5221 catttcgata gtcctcgccc acatcgcgca gcacgttggt gacctgtagc gcgacgccta
     5281 gcttgatcgc gtacgggatg gcttcagggc cggagaagcc ggtgatgtgc atactcatca
     5341 gcccgacggt cgaagccaca ccatacgagt aagccgccag atcgtcgaaa gtctcgtaac
     5401 gctgctggag cagatcgcgc gccacgccgc cgatgagctg ctccacgtag cggtgcggaa
     5461 tacgaaagcg ccagagcgta tccgcccacg ccacggccac gggatcgtct tccggcgggt
     5521 ggggcatcag cacgcgctcg cgccacgccg ccagcatggc ttcagcgtcg ccgctcggcc
     5581 tgtcgacgac gtcgtcgctg gtgcggcaga aggcgtataa cgcgcgcacc gcgcgccgct
     5641 tgtcaaccgg aagcaactgc gacgccatat ggaacgagcg gctgtgctcg gccgtaattg
     5701 cttcgcaatg agcataagcc cgttgcagga gtgctgcatt ttggacaggt tccctgtgta
     5761 tcgatagtga ttgccacgct tcatgtgcca gcaacagtag gctgtgttcc caagtctggg
     5821 cctgtaagat catttcgccc ccgtgtcgac gtactgcctg taagtcagcc agcaggataa
     5881 tatcacaaat gaacactatt tgtcaaggta ttgctttgca aatacttgac aagaatcgga
     5941 atgaatgata atgttggcta tgcaaggaga caatttccat gacaagacga cttacggcat
     6001 tggtaattgg cgcgggaatt ggcggcattg cgaccgctgc gcggctggtt cgaaatgact
     6061 ttgacgtgac ggtggtggaa aagaatgccg agcctggcgg acgatgtggg cggcttgtgc
     6121 gcgatggaca ccggttcgat atcggcccca ccctcttcct aatgcccgaa gtattcgcgg
     6181 aaacttacgc ggccctgggc gagcgcatgg aagatcacct cgatctgcgc cgcatcgacc
     6241 caacctaccg catccgattc gatgatgacg ctgtcctcga tctcaccgcc gaccttaacg
     6301 cgatgcaggc gcagctagag acgatcgagc cgggcagctt tggcggtttc ctgcgttacc
     6361 tggcagaagg caatcgccac tacaagacct cgctgacaca tttcgtgggc cgcaattttt
     6421 acagtccact acaatacttc agcttgagca acctgccgtt gctgtttcga ctgaaagcgc
     6481 tggtcaagca ttaccacaac gtcggcaatt acttcaagca tccgcatctc aaggcggcat
     6541 tcacgtttca gaacatgtat ctgggactca gcccatatga tgcgccggcg acatattcac
     6601 tgctgcagta caccgagctg gccgacggcg tgtggttccc catgcgcggc atgtatcgca
     6661 tcatcgagac actggtctcc atcgccgaag cgaagggtgt gcgctttatc tacaacgcgc
     6721 cggtcgagaa gattgaggtc gagggcaaac gtgcgacagg cgtcgtgctg aacgatgggc
     6781 ggcgcctcac ggcggacatc gtcgtcgcca acgccgatct gccgtacgtc tatcgtgaac
     6841 tgctgccgga tcgcgccgag accgatcggc tcgatcgcat gaagtacacc tgctcggcaa
     6901 tcatgttcta ctggggcgtc gacaaggtct atccacaact cgggcatcac aatgtattcc
     6961 tggccggcga ttaccgcgcc agcttcgacc gcatcttcaa cgatgacacg ctgcccgacg
     7021 agccgagttt ctacgttcac gcgcccgcgc gcactgaccc gtccgccgcg ccggtcggtc
     7081 acgacacact gatggcgctg gtgccagtcg gtcacatcga cgaatcggct ccgcaggact
     7141 gggccgcgct tcaagcccgc gcgcgatcgg tcgtgctgca gcgattggcc cggatcggcg
     7201 ttagcgacct cgaatcacgc ctgaagttcg aagtgagtta cacgccgcgc gagtggcaga
     7261 gcctgtacaa cctggccaag ggcgcggcgt ttggactgag tcacaacttc acccaggtcg
     7321 gatacctgcg gccgcagaac cggcatcgcc gttaccgcaa tctgtacttc gtcggcgcga
     7381 gcacgcatcc cggcacgggt ctgcccatcg tcctgctgtc cgcgcgactg acgacggagc
     7441 gaattctgaa agaggccggg ctgccgcagc ccacgctcgt ggcagaacct gtagccgtat
     7501 agcactgaat gggtcaatct ggacaaatgc tatacaattg acttatccct cagggctgac
     7561 accgtggagc agtgatttgc cgtaatgtca ccgccgcaat aaccacgcga gcctgaagag
     7621 agctcagcat gccgcgtaaa gaaggacccg tctacaacct gaaggctgtt atccgcgaga
     7681 ccggactaaa gccggacacg ctgcgcgcct gggaacgccg ctacggcatg cccaagcccg
     7741 atcgcacacc cggcaagcac cgcctgtact cgcagcgcga catcgacacg ctcaagtggc
     7801 tgatcgcccg ccagcaggaa gggctgagta tcagccgcgc cgtcgagctg tggcgcagca
     7861 tcgcagccga tgggcaggac ccactgcaaa gcagcccggt tgcttcgacc gtgaaggcat
     7921 cgttgtctgc gctggcatat ggcgacgcca tcactgactt gcgtgccgcc tgggtatcgg
     7981 cctgcacaga ctacgacgag cagcgcgcca agagcgtgct gactcaggca tttgcgttgt
     8041 atcccgccga gacggtctgc attcacgtct tgcagaaggg attggcggac atcgggcaag
     8101 gctggtacga aggctcgatc accgttcagc aggagcattt cacttcggcg ctggccttgc
     8161 gccggctcga tgcgctgatc gcggcgatgc ctgcgcccac gcgatccggc tgcattctgg
     8221 tcgcctgtcc ggcgcacgag gagcatacgt tcggcccgct gctgatcacg ttcatgctgc
     8281 ggcgccaagg ctgggatgcg ccgttcctcg gcgcgaatgt gcctctggcg cgcctggaat
     8341 cggcgttggc ggccacccgg gcgaagctcg tcgtcgcttc cgcgcagcaa ctgcacaccg
     8401 ccgccaacct gctggacatg gcccatcttc tccgccgcga gaaagtggcg ctggcattcg
     8461 gtggcctgat cttcaatcgc gtgcccggcc tgcgcgatcg cattcccggc tacttcctgg
     8521 gtgagacgct ggaagaagcg ccgcaggccg tcgagcgaat catggagtcg ccgctgcctg
     8581 cgcctgccgc cgcgcctgcg ctgccggagc atcgggccgc cctggtggac tatcgcgcca
     8641 ggcgtgcgca gatcgaggcc gatgtgctaa acaacatcgc gccggaagtc cggcagcaca
     8701 actacctcga cgtggcaacc gccaatcttg cccacgagat cgaagcagcg ctgatcctgg
     8761 gcgacatgga gttcctcgac agtgacatcg cctggataga gggactgctg atcagtcacg
     8821 gcatcccgcc cagtgtgctg cattactttc ttgacgctta ccggcaggcc gtcgaatcgc
     8881 acatgggatc ggtcggcgcg ccgatcgcgg cgtggctggc gaccatcgcg cccgggtaga
     8941 ttaccgaccg cccagtgccg accgccgacg attgacggcc gtcggctgtt ggtagtcttg
     9001 atgaggagat atcgctatga gagtcgttat caccggtgga acaggcctga tcggccgcgc
     9061 gcaggccgcg agtctggcgg ccgataagca cgaggtcatc gtcctcagtc ggagcccaca
     9121 gtacgccagg tcgatgcccg gcggcgtgaa ggttgagaag tgagacggcc gcacctcagc
     9181 gggctgggga caattcgtcg acggcgcaga cgcggtggac aacctggccg gcgcgatccg
     9241 cttcctggtc gacaagcccg atgcgcgcgg cgcgttcaac ctggccgcgc cgaactctgt
     9301 gacgaacgcg caactagcca aaatcctggg gcgagtcatg cgccgtccgt cgtatttccc
     9361 cgttccggcc tttgtgctgc gtgtgctgct gggcaagaag gcgacactgg tgctcggcgg
     9421 cgcgcagatt gtgcccaagc ggttgatcga cgcgggctac accttcaagt tccccgaagc
     9481 cgaagccgct tttcgaggcc tgctcaagtg aagtaccggc atcagtttcg cgttcgcgcc
     9541 ccgctggccc gcgtggccga gttccacagc cgcacagccg gtcttgctct actgataccg
     9601 ccgccgatcg tggcgcggat acatcgcgcg ccgatcatcc agcgcgaagg cgacgaggta
     9661 gatttcacga tgtggctcgg cccgctgccg attcgctgga tcgcgcgcat cgatagcgtt
     9721 tccgaaaccg gcttcgtcga tcgtcaagtg cgcgggccgt ttgaacgttg gctgcactgt
     9781 cacaccttca caccgatcga tgccagcacc accgccgtga ttgacgacgt gcaggctgtc
     9841 ttgaaagcgc atcgggggtg gggagcggtc ggcttgacga tgtggctggc gctgccgctg
     9901 ctgtttgctt atcgcggctg gaagacccgg cgcgcgctgg aaacgaaacc gacatgagcc
     9961 gcatcgtcgt tgtcggcggc ggcgtcggcg gtctgacgac agctgccgtc ctgtcgcgcg
    10021 ccggtctgga tgtgaccgtg ctggaagcgc acgtctatcc gggcggatgc gcgggtacgt
    10081 tctatcatca gggttatcgc ttcgacgcgg gcgcgacact ggcgggcgga ttctaccccg
    10141 gcgggccgat ggatttggtc gcgcaggcgg cgggcattga ggcgtggcct gcccgatccg
    10201 ccgacccggc gatgaccgtt catcttcccg atcgcgcggt tgttgtccgg cgcagcggcc
    10261 cgcccgaccc cgttgaacag cgagagtttt tcggccctgg cgcagacgcc ttctggcgct
    10321 ggcaacagcg caccgccgat gccctgtggg atttcgcgct gcggctgccc gcctggccgc
    10381 ctcaatcgcc gatcgatctg gtgcgactgc tcagcgacgg cgcaggctgg gcaagcgcca
    10441 atctccacac tctctatccc gatctcgtcc tcgacgcatt ccgatccgtc gccgcgcatc
    10501 ttcatcacgc atccgatcgc ttgcgggtgt tcatcgacgc ccagctgctg atctcggcgc
    10561 agaccaccag ccgatacacc aacgcgctgt atggcgcatc ggcgctggac ttgccgcgtc
    10621 ggggcgtggt tcacctgcaa ggcggcatgg gcgcaatcgc tcacacgctc gccgatgccg
    10681 tccgtcgcaa cggcggcgag gtgcgctatc ggcaggaagc gacgcgcgtc gtgatggaga
    10741 acggtcgccc ggttgcagtc gagacgaagc gcggcgattc gttcccggcg gacgtcgtcg
    10801 tcttcaacct gccgccatgg aacgtcgccg gtctgctcgg agataatgcg ccgcctcgat
    10861 tgcgcgcact gccctcacac ccgcgcgatg gatggggcgc gttcgtggtg tacgtcgggc
    10921 tggatggatc ggtcgtgccg ggcgacgcgt tgctgcatca ccaagtgatc gttcgcaagt
    10981 cgctggccga aggcaacacg atcttcctgt cgatcagccc ggaatgggat cacgaccgcg
    11041 cgccgatggg caagcgcgca ctgacgatca gcacgcacac cgacctggac tcgtggtggg
    11101 gtttgtttga gcgcgatcgc gccggatacg acgcgcatgt gcgcgagtac accgatcgcg
    11161 ttgtggccgc cgccgcgatc gtgttgcccg gcctgcgcga tgccgccgag ttgatcctgc
    11221 ccggcacgcc cgtcaccttc cagcgcttca cgcgccgaat cggcgggtgg gtgggcggct
    11281 ttccgcagac cagtcttctc cgagcctggg gaccgcgact cgcaccggaa atgtggatgg
    11341 ccggcgacag catcttcccc ggccaatcga ccgccgccgt ggcgatgggc ggtctgcgcg
    11401 tggcgcgatc ggtgtcaggc gagatgggaa acatcgcggg ccaagattcg tcagatgatc
    11461 tcgttcacgt ggcggcgatt gatcgtgaac cccgatcaac cgttgccggg acgatccggc
    11521 gaagcgatgt atgccggttc gattcacgcc gctccaggag cgcgctggat ctgtggcgtt
    11581 tccgtaggag cagaatgaag gggaagcgat ttcgattcta aggagaagaa gctgcatgac
    11641 ccaggtacct ctcgttctgg tcaccggcgc gacgggatac atcggcgggc gtctggcgcc
    11701 gcgattgctg gaagcgggtt accgtgtgcg cgtgttggcg cgagatccgg cccgcttgca
    11761 gggacgcgag tggttggatc gggtcgaagt tgttcgcggc gacgtgctga aggccgagac
    11821 actgccgtcg actatggccg gcgtcggcgt cgcctactac ctggtgcaca gcatgagcga
    11881 cacggcggac tttcaccagc gtgacatggc cgccgcgcgc aacttcgccg aggcggcccg
    11941 cgccgccggc gttcagcgca tcatctacct gggcggcctc ggcaatcccg acgccgattt
    12001 gtctcatcac ctgcgctcgc gccaggagac gggtcaggcc ctgcgcgagg cgggtgtgcc
    12061 ggtcacggag ttccgatcgg ccatcgtcgt cggctcgggc agcgtctcgt tcgagatgat
    12121 ccgttacctg accgagcgca ttccgctcat gatctgcccg cgctgggtgt acacgcgcgt
    12181 gcagcctgtc tcgatccgca acgtcctcga ttactttgtg gctgcgctga acacaccgga
    12241 aagcgcgggc cggatcatcg aaatcggcgg ggcggatgtg ctgacctacg gccagatgat
    12301 gaccggctac gccgaggtgc gcggcctgcg gcggtggttg atgccggttc cggtgctcac
    12361 gccgcgcctg tcctcgtact gggtgcacct ggtcacgccg atccccgcag ccattgcgcg
    12421 gcctctgatc cagggtctgc gcaacgaagt cgtcgtgcgc gatgactcag cccgccgtct
    12481 attccccgcc gtcgatctaa tcgactatcg cacggcagtg cggcacgcgc tggcggatct
    12541 ggacgccggc tcagtggaga ccgcctggag tgacgcgctc gtcaccagcc agggcgacgc
    12601 agcgccggtc gtgctggcca cgcaacaggg catgattatg gagcagcggc ggcgcatcgt
    12661 ggccgcgccg ccggacaagg tctacgcggt gttcgccggg ctgggcgatc ggcgcggctg
    12721 gccgtatgcc aactgggcct ggcggctgcg cggcgtcatc gaccggctgg caggcggcgt
    12781 cggactgcgc cgagggcggc gcgatccgaa cgatgtccgt gccggggacg ccgtcgactt
    12841 ctggcgcgtc gaggaagtcg tcttggagcg caagctgcgg ctgcgcgccg aaatgaaggt
    12901 gcccggccgc gcctggcttc aattcgaggc gcagcccttt tccggcgagc ggacgttatt
    12961 gctgcaaacg gccttcttcg cgccgaaagg cctgttcggt ctgatgtact ggtatctgct
    13021 gtatccgatt cacagcctga tattctccgg aatgattcgt cgcatcgcac agcgcgctga
    13081 gatggccgcg ggggccgagc gctcgtgacc acagctatct ggtggatccg ccgtgatctg
    13141 cgtttgtccg acaatcaagc cctggccgag gcgctggcct cgacggatcg aatcgtgccc
    13201 gcgttcgtct tcgatccgtg tctgctggcc tcgccctacg tcggatcgaa acggctggca
    13261 tttctgctac acggcttgcg cgcgctggat gttgatctgc gcgccaaagg cagccgcctg
    13321 atcgttcggc gcggcgatcc agcggttgag cttgctgcgc tcacgcgtga gagcggtgcg
    13381 gcggcgattt tcgctgaaga ggacatctcg ccgtacgctc gcgcacgcga tgcgcgcgtt
    13441 gccgagcgac tgccgctgat gcagactcgt ggcttgacgg cgcagctacc gccgatggtg
    13501 tcgaagccag atggcaagcc gtacacagta ttcacaccgt tcagccgcgc gtggaaggcg
    13561 ctgccaccgc cacaggtgcg cgacatcatt gccgcgccga agcgcattga aacgcctgac
    13621 gatcttcaca gcctgccgat tccgccacat ccggcgctgc ccgcatctgt tccgttcgtt
    13681 tcgggcgagg ctgaggcgca gcgaagactc acggcgttcg ttagctcaat catcggcgag
    13741 tatgccgatg tccgcaatcg gatggatcgc gacggcacat cgcagctctc gccgtacctg
    13801 cgattcggca tgttgtcggc gcggcaggcg atcgtcgccg cgatcgaggc gatgcgatcc
    13861 gcggcgagcg cggcaataca ccggggcgcg gaagcctggt tcaacgaact gatctggcgc
    13921 gagttctaca tcaccatcct acatcacttc ccgaacgtcc gccgtgagag cttccgcccg
    13981 gagctgcgca atattccatg ggagaacgac gaaggcaagt tcgcggcgtg gtgtggaggt
    14041 cggaccggct atcctgtcgt cgatgcggcg atgcgccaat tgatcgagaa tggttggatg
    14101 cacaaccgcg cccgcatgat cgtggcttca tttctgacga aggacctgct cattgactgg
    14161 cgctggggcg agcggtggtt tatgcagcat ctgctcgacg gcgatcccgc cgcgaacaac
    14221 ggcggctggc agtggacggc cggcaccggc accgacgctg caccgtactt ccgcgtgttc
    14281 aatccggttc tgcagggagc gaagttcgac ccggatggca cgtacgtgcg tcgctgggtt
    14341 ccggaactgg cgagtgtgcc cgatcggttc atccataagc cgtgggacat gccgcacgag
    14401 atgcagtcat ccatcgaatg tggcatcggg cgcgactatc cggcgcccat cgtcgatcac
    14461 gaacaggcta gggagcgcgt tctggctgcg tacagggaag cgaaagccaa ggtttagtgc
    14521 ggtttttttg tgcgtcctga actctgtatc cctcatcgac agatgatttt acataatgca
    14581 aaatgagaat ctgataagta tatgttcaaa tattgtgcaa gatattgaca aatactgaat
    14641 ttcgtgatag aatgttggaa atccagaaca ggagagaaaa gatgaagcga tttaccgttg
    14701 tgagtatggt tttggtcctg gctctggcac tgaccagcag tgcttttgcc gagacgagcg
    14761 cccgcgtccg agtagtgcat gcctcgccgg acgcgcctgc tgtcgacgtg ctggtgaacg
    14821 atgctgtggc gttcagcaac gcgccgttca aggggattac gcagtacgca tccctcgatc
    14881 cggccaccta caatgtcaag gtcgttccca cgggagcgac cgagccggtc gtgatcggcg
    14941 ccgatctgac gctggaggcc aaggactaca cggtcgtggc tgtcgataca ttggcgaaca
    15001 ttgagccgct gatcctggtc gataacaaca gcgctccggc ggccggcaag gcgcacgtcc
    15061 gcttcgtgca cgcctcgccc gatgcaccgg cggtggatat cgccgtagca ggcggtggcc
    15121 cggtgctgtt cagcaacatc gccttcaagg gcgtaggcga ctatctgccc gtaggtgccg
    15181 gcacgtacga tctggaagtt cgcattgccg ggaccgacac cgtggccttg agcgtgcccg
    15241 gcgtgcagtt gaacaacggc acagtctata ccgtcttcgc gatgggtctg gctggcggca
    15301 cgccggcgct gcaggcggtg cccagcgtcg acgcgacgcc ggtgcttctg ccgcagtccg
    15361 gaggcgaatc ggcgggcaac ccgtacctgc tgatcgtgct gctcggcgcg gcgctcctgg
    15421 caggtggtct gatccttcgg attcgaccgg cgctagcctc ggccagaagc cagaacgacc
    15481 gctagtcggg tggagtgggg ttttactggc atgggttgag tctcttactg taacgggctg
    15541 ggtgtttatc ggtaagaagg gggtgtgtcg cggtggttga cccaccccct tcgctgggtc
    15601 gacatagatt ggcatcggtc agccggcagg ctacatatgt tacaatctga gaagcgagaa
    15661 acatgaaacg ttttcgccgt atcgcactca tcatcatcgg actcattctg gccgtcgttt
    15721 tagttgggcc gtttctggta ccggtgcctc ccctggaggg caccgttccg cccgagcaac
    15781 tggccgatag cgatagccgc ttcatcgacg tcgacggcct gcgcgtgcac tacaagactt
    15841 cgggcgacgg ctcgccgact ttcgtgttgc tgcacgggtt tggggcgagc gttttctcat
    15901 ggcgcgaggt gatgactccg ctgtctgagt cgggcacggt aattgccttc gaccggccgg
    15961 ccttcggcct aaccgagcgt cccctgccgg gcgattggga tggcgagagt ccctacagcc
    16021 cggaggcgca aatcgagctg accgtcaagc tgatggatca gctgggtgtg gacgaggcga
    16081 ttctggtcgg caattcggca ggcggcacca tcgccatggc gacggcgctg gagcacccgg
    16141 atcgcgtact ggcggtggta ctggtcgatg cagcaatata caccggaggc ggtacgcccg
    16201 actgggttcg cccactactc ggtacgcctc aaatgcgccg tatcggcccc ctgctggcac
    16261 gcagcattca gaatctggga ccgaacctga tcgagagggc ctggcacgac ccgtcgaaaa
    16321 tctcagccga aatcttggaa ggatacacaa agccactcaa ggtcgacaac tgggatgtcg
    16381 ccctgtggga attgacgctg gccagccatt ctttaggact ggatcggcgc gtgcgcgata
    16441 tcagggtgcc ggtgctggtc atcaccggcg atgacgaccg catcgtgccg accgagcaaa
    16501 gcgtgcggct ggacggcgag ctgcccgacg ccgaactggt ggtcatacct aattgcggcc
    16561 acgtgccgca ggaggaatgc ccggccgagt tcacgcaggc gatcgtcgat tatctgccgc
    16621 agctgacggg cagtgcgtca ctgtccggac cgcaatgagc gataaaggac agtcgagata
    16681 caattggact gtgccgccta ctatggcgaa ttcattttcg tgagagagga cagcatgaac
    16741 aaggatagtt tgcgacaggc ggtcaacacc atcacgttct ttgccaccgt cgtcgtcaac
    16801 gggttggcga acgcgctgcc gttgaacggc cagacgaccg cccagatttc ggatcggttt
    16861 ccggtgtact tcacgccggc caactacgtg ttctccatct ggggtgtcat ttacctgctg
    16921 ctgctcggct tcaccctcta tcaggcattg ccctcgcagc gagagaatcc gcgcctgcgc
    16981 cgaatcggct acctgcccgc gctggccggt gtattgaaca gtgcgtggat atttctgtgg
    17041 cactacaacc tgtttgagtt gaccgtgccg gtgatggccg gcctgttgct cacactcatc
    17101 gccatctatc tgcggctgga cattggccgc gcggcgacaa ccatacgcga gaagtggctg
    17161 gtgaacatcc cgttcagcgt gtacctggga tggatcacca ttgccacgat tgcgaacgtg
    17221 acggcgctgc tgtattacct gaattgggac ggcttcggca tcagccccga ggtgtggacg
    17281 gtcgttatcc tgctgatcgg cgcggcgatc gcctcgatgc tgatcttcac ccgcgccgat
    17341 gtcgcttaca cgctcgtcat cgtgtgggcc tacgccggca tcgtcgtcaa gcagttcgga
    17401 gtgccgatcg tcgccggggc ggcggtgctg ggcgcgctcg tcgtcattgc tgtcctgatc
    17461 gccgcgcggc tgcagcgggt acaacccggc tgaaggcaga tggccgcttc ttagcttcgt
    17521 tttcacgggc atcatgtggg tcaagcccac gacgctgtat catccgccag agcgtacgcc
    17581 atcgccggca ccagcgtcat cgaccggctg gcggcccatg ccgcggcaaa ggctgtttcg
    17641 tccagttggg agcgcacggc ggccacaaca cgctcatatt cgatgttgtc tgcttgcagc
    17701 aatcgagtgc cgacgtcgtt gagcagggct tcgatgatcc cgaagagctt cgccgcacgt
    17761 cgaaaatacg cgtggcgggg tggcccatcc tcgacgcgaa gcgaaacggc ttgtcctcgc
    17821 gctacagcca cgccggccaa tgccgcgaga caggcggcca tgccctgctt gtccccgact
    17881 tccatgttga ggccgaggct ctcgcggcac agcgccgtgg cctccttagt gtcgccccga
    17941 tacagtgcca gatatcctaa gcgacgaacc gggtaggcca gggcgctctt ggccatgaac
    18001 tcgcgagacc aggtcacggc ctcttcgtat aacgcctgcg cgcgctcgaa gtcttccata
    18061 tgcacagcag tgtcacccag gaaggtgagc agccaaccga catgccactg gctttccagc
    18121 tcgcgccaga cggcgacacc tctctccagg aacgaacgtg ccgccggata atcgccctga
    18181 ttgagcgccg ccgtacccaa cagacgaagc gccaggccgc tactcggttt atctcccagt
    18241 tccataccga tcgccagggc ttcttcaagc aggcggcgcg cttccgtatg gtcaccccag
    18301 gtcgacagga gggcgccggc ggcattcagc agcttcatgc gctcgagcgt tccagacggc
    18361 gccgtcgcct ctacatgagc cacgatttcc cgtaatcgct ccttcctctc accgaggtaa
    18421 cctcgcatcc accacaacca cgacatggta ctcgccagct gcaacgccgt cgatgcatct
    18481 tcactctcca gcgaccattc gatggccgct cttaagttgt cgtgctccat ctcgagcgat
    18541 tctgcctcgc ccccagcatg gtaatacgcc tcatgctctg ccgcgagtct cacgaagaaa
    18601 tcgaggtgac gccgccgcgc caggttcaat tcccccgcct ccatcgtctt tcccagccca
    18661 tactcacgaa tcgtttccag caggtgattg cgggccgcct gattctgatg gtccacgatc
    18721 accagcgacc tgtctactaa ctgtgacagc aggtcgattg ccgcccggcc actcatcgcc
    18781 ggatcatgca acacatcggt caccgcctcg gcggcccacc caccccggag caggtagccg
    18841 aactgcacaa gctggacaaa gagatgtcgt tcatcggacg cgtggacttc gtcctgctgg
    18901 tggtggctct gctgacgatg acgacggccc gctactggta tgtgtgaact tgtcagcgga
    18961 cggagtgaga cacggtcaac ggtcgactct gggagggcac gtctgccgct tgcggcgatt
    19021 cccagggccg gcagcgggcg ggcagcgctt tgccggattg cgcgatgaaa catcacgatg
    19081 acaacgaacg acagggacga gcgccttcat ccccgtgcgc tgcctcaagc aaactggcac
    19141 actctggccg aattcaccgc gcgcgccaca cgggacagcg aacgccaggc ggccgaacgg
    19201 gttgccagga tggtgcgtga attgagtctg ccgccggagt tcgtggaacg gatcaagacg
    19261 gccgtagccc acgcgatgct acatgcgatt agacgtgaca ccgaccagtg gcccggagcg
    19321 tcggtgggca tccgcatact gatctctgcg gcagcgccgc cagcgggttc agagccgagg
    19381 cacaagaacc agggttgggg attcttcctg atcagcaggg taagcgacgg cccgcagacc
    19441 gaaccggcca gtgcccgacc tgccatcgag ttgtatcttt atcctgaagg caatgcgagg
    19501 gagaagtgat ctgaggcagc ccggcagggc gatcaaggag caatgatatc cgccgcgcgt
    19561 ccgtttctcg cttcaagcat atcgccaccc ggcgatgtgg gattatggca atgacgatct
    19621 gggatattcc ccgccaaatg cgattgtcct cggcggtcag gactttcgac ggag
//
LOCUS       DBNHXB010000187         4864 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4572501, whole genome shotgun sequence.
ACCESSION   DBNHXB010000187 DBNHXB010000000
VERSION     DBNHXB010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4572501"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="AC162G_07560"
     CDS             complement(<1..1337)
                     /locus_tag="AC162G_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075705.1"
                     /translation="MHNPQPDADEAPVELTPGAGGPSGFQSMWGNPRHRRRLILGGIA
                     GVLVILLIVLLSSQGGRPSPEATPTPSDLTAVPVTLSIKDRSFTVAPVTVTDGPWRVS
                     QGDASSAEWIYGTVINYIFGLLPTPETTELMEGLADDDRITLDMSTGGQLSFRVSGRQ
                     RVPGDAVPDLFQQTRPGITLVLLREGGDERLVVTGLYDADLESLSAPGTSRVPVGMPA
                     QVDKWRVTVLSGQLAPDPTNPAQANYYVNFTVEYLGDQPVSSDTFEMTLMDGVGVAYD
                     FDPQVSRLSAYSPPGGLVAPRNPTPFTAGYRVSTDIPGPTLEWVFKPTPGTVQPARFE
                     VPIVRPTPTPEPKTQITVQINGASLSTDQTLLVVTGGIGNPTDQSVSIGPADISLSTP
                     DNVFSDVRTAEPALPWTIGPGQTMAFRLEFNRPPGFAAILRIVTQRFEFSGLR"
     gene            complement(1350..2948)
                     /locus_tag="AC162G_07565"
     CDS             complement(1350..2948)
                     /locus_tag="AC162G_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075706.1"
                     /translation="MSRLQAIALAGIIAVSAALALSLLMVASAQAAPGLSNVAAHGQA
                     LGVTAGSAPGVTGFELTRPGKHDPLSIESIIAAQTPLTCTYTVTTPVSDVTNNFAFTN
                     AAALFTYNNLTLLPSNAPASPSSPLTVTVATQYFRVDALQGFHYLVSAIPNSLSSYNL
                     LIVVYNVSQTPILTSTKSLGSLSASVDFVPSSSGTYYFAISHIASNCPSGTYKLTADG
                     PLANTATPTRTPGPSATPTSPVTPIPGADRFEPNFNFDRASLLALDVKYTNLNFAPWA
                     GSDPNAPDNDFYKVYVKPGLLVTCETLELGPGVDTNLILYDNNTNGIGGSEDIDRASG
                     NFASRLTYYVTYEGWLYALVGNVYQIDPTVAASYSYSFQCYIGGGPTATPTVTRIPVT
                     PPTPIPTNTPTPSLVPTLTPTPTPTPPFIVVNEVATATPVGQQTVVVPISLVVYYDLN
                     DNRSPDPGEGVVGISARVIDVITGQELRHGFTDANGFVSLTTQATGVVRLVVPYLSYS
                     VVIQPSGSQITLRIAPSGLPDSIP"
     gene            3315..>4864
                     /gene="recJ"
                     /locus_tag="AC162G_07570"
     CDS             3315..>4864
                     /gene="recJ"
                     /locus_tag="AC162G_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR5075707.1"
                     /translation="MLSDNPPPASTEPRAEPVSRLKKRWEIAPRAPAGHFALLPDLPP
                     LLVQVLYNRGLKEPGPIAEFLGDDAPPADPFLLKGMGDAVVRLRRAIVQNEPIAVYGD
                     YDVDGVTATALLTQALRGWGANVTPYIPDRFEEGYGLNAGALGKLSAQGAQVVVTVDC
                     GARALDEVAHANRLGLDVIVTDHHEPEGDRLPAARALINPKQPGCPYPDKHLAGVGLA
                     FRLAQALAWELARDGSSLPFPIDDLLDLVALGTVADLAPLVGENRDMVRRGLRLMNAG
                     SRPGVVALCNVAGQQPGELDAGSIGYSLGPRLNAAGRVEHARAAYQLLVSPSVEQAAQ
                     FAYQLNEQNRERQRLTAEMVDAAAAQALAGDPEAPILFAASAGFSPGVIGLAASRLVE
                     VYHRPAIVMSVGEGEARGSARSIPQFHITHALDECKDLLVKHGGHAAAAGFSVALDCI
                     DELKARLTSIAAARQAEGDWSPVLKADALVRLSTLTYEMVRHLARLEPYGMQNPQPVF
                     VSRDIRVRG"
BASE COUNT          869 a         1498 c         1632 g          865 t
ORIGIN      
        1 tatcgcagcc cgctgaactc gaaccgctgc gtgacaatgc gcaggatggc ggcgaagccg
       61 ggcggacgat tgaactccag ccggaaggcc atcgtctggc ccggcccgat ggtccacggg
      121 agcgccggct cggcggtgcg cacatcgctg aagacgttat cgggtgtgct caggctaatg
      181 tcggccgggc cgatcgacac ggactggtcg gtgggattgc caatgccgcc ggtaaccacg
      241 agcagcgtct gatccgtgct cagcgaagcg ccgttgatct gtaccgtgat ctgcgtctta
      301 ggctcaggcg tgggtgtggg gcgaacgatc ggcacctcga agcgcgccgg ctggaccgta
      361 cctggcgtgg gcttgaagac ccattcgagc gtcgggcctg gtatgtcagt tgacacccga
      421 tagcctgcgg tgaacggcgt tgggttgcgt ggggcgacca gcccgccggg cggggaatag
      481 gcgctgagcc ggctgacctg cggatcgaaa tcgtatgcca cgccgacgcc gtccatcagc
      541 gtcatttcga atgtatcgga gctgaccggc tggtcgccca aatactcgac ggtgaagttg
      601 acgtagtaat tggcttgcgc cggattggtc gggtcgggcg cgagctggcc cgacaacaca
      661 gtgacgcgcc atttgtcgac ttgcgcgggc atgccgacag gcacccggct ggtgccgggc
      721 gcgctaagcg actcgagatc ggcatcatac aggccggtga ccaccaggcg ctcgtcgccg
      781 ccctcgcgca acagcaccag cgtaattccc ggacgcgtct gctgaaacag gtccggcacc
      841 gcgtctcccg ggacgcgttg gcggcccgac acgcggaagg acagttggcc tcctgtgctc
      901 atgtcgagtg taatccgatc gtcatcggcc aggccttcca tcagttcagt cgtctcgggg
      961 gtcgggagca gtccgaagat gtaattgatc accgtgccgt atatccattc ggcgctgctg
     1021 gcgtctccct ggctgacgcg ccaggggccg tcggtgaccg tgaccggcgc aaccgtaaaa
     1081 gacctgtcct tgatcgacag ggtcaccggc acggcggtta gatcgctggg tgtgggagtg
     1141 gcctccgggg atggccggcc gccctgcgag gacagcagta cgatcaggag gatgaccaag
     1201 acgccggcaa tgccacccag gatcaagcgc cggcgatgcc gaggatttcc ccacatggat
     1261 tggaagcccg acgggccgcc ggcgccgggc gtcaattcga ccggcgcttc atccgcatcg
     1321 ggctgagggt tatgcacaga ggctcctcgt caggggatcg aatccggcag gccagatggc
     1381 gcgatgcgca gtgtgatttg actgcccgac ggctgtatca ccaccgagta gctcagatag
     1441 ggcacaacca gcctcaccac gccggtcgcc tgcgtcgtga gcgagacgaa tccgttcgcg
     1501 tcggtgaaac catgccgcag ctcctggccg gtgatcacgt cgatcacgcg ggccgagatg
     1561 ccgacgacgc cttcgccggg atcaggactg cgattgtcgt tgagatcgta gtagaccacc
     1621 aggctgatcg gcaccacgac ggtctgctgc ccgactggtg tcgccgtcgc cacttcgttc
     1681 acgacgatga acggcggcgt cggcgtgggt gtgggtgtaa gagtcggaac gagcgacggc
     1741 gtgggcgtgt tggtcgggat aggcgtcggc ggcgtcacgg ggattcgagt cacagtcggg
     1801 gtggccgtcg gaccgccgcc aatgtagcat tgaaaactgt aagagtaact tgcggcgacc
     1861 gtcgggtcga tttgatacac gtttccgacg agcgcgtaca gccagccttc gtacgtgacg
     1921 tagtacgtca ggcggctggc gaaattgcca ctggcgcggt cgatgtcctc actgccgcca
     1981 ataccgttgg tgttgttgtc gtacaggatc aagttggtat ccacacccgg gcccagttcg
     2041 agcgtctcgc acgtgacgag cagcccgggc ttgacgtaca ccttgtagaa gtcgttgtcc
     2101 ggcgcgtttg gatcggagcc ggcccacggc gcgaagttga ggttggtgta cttgacgtct
     2161 aaggcaagaa gactggccct gtcgaagttg aaattcggct cgaagcgatc tgcgccggga
     2221 attggcgtca ctggactcgt cggcgtggcg cttgggccgg gtgtgcgcgt tggcgtggca
     2281 gtattcgcta acgggccgtc ggcggtcaat ttgtacgttc cagaggggca gttcgaagcg
     2341 atgtgagata tggcgaaata atatgtgcct gagctggaag gcacaaaatc gacactggcc
     2401 gacaagctgc cgagcgattt ggtactggtg aggattggcg tctgactcac gttgtaaacg
     2461 acgatgagta gattgtagct actcagcgag tttggaatcg ctgagacgag ataatggaag
     2521 ccttgcagag catcgactct gaagtattgc gttgcaacgg tgactgtgag aggtgaggaa
     2581 ggtgaagccg gggcgttcga cggcaatagc gtaaggttgt tgtaggtaaa cagggcggcc
     2641 gcgttggtga acgcgaaatt attggtcacg tcgctcacag gcgtcgtcac cgtatacgtg
     2701 caggtgagcg gcgtctgggc agcaataatg gattcgattg acaacggatc atgtttaccc
     2761 ggcctggtaa gctcaaagcc ggtcactccc ggagcactcc ccgctgtcac ccctaaggcc
     2821 tggccgtgtg ccgcaacgtt ggacaggccc ggcgcggcct gggcactcgc aaccatgagc
     2881 agcgaaaggg cgagcgcagc cgaaaccgcg atgatgccgg cgagggcgat ggcttgcagg
     2941 cgagacatca ggctatccct ggaacccgaa ttcgctcaca cgcgaccaag tatcaggctt
     3001 ttccaaagtc aagtaatgat gctcaccgcg ccgtcccttc gcccggttca gggcaggcct
     3061 cggcacgggt cttatgacgc cctcgccacc ggggacggcg gcttgagcga ctctggaaaa
     3121 gctgtgaacc aagtataacg caagttgaaa agcgcgcaaa tccgtgctac tattgccgcc
     3181 agccgtagac cgcagcccgg cgtgggtttg cgccagattc tcatggcgag caacacccca
     3241 ctcctcacca attaccaatt accgattact gatcactgat tactgctcac tgtccagttc
     3301 gcgcccgaca tcccatgctc agcgacaacc caccccctgc gtccaccgaa cctcgcgcgg
     3361 aacccgtctc gcgactcaaa aagcgttggg aaatcgcccc tcgggcgcct gccggccatt
     3421 ttgccctctt gcccgacctg ccgccgcttc tcgtccaggt gctgtataac cgtggcttga
     3481 aggaaccggg ccctatcgcc gagttcctcg gcgacgatgc gccgccagcc gatcctttcc
     3541 tgctcaaagg catgggcgac gccgtcgtac gcctccgccg cgccattgtg cagaatgagc
     3601 cgatcgccgt ttacggcgac tacgacgtgg atggcgtcac ggctacggcg ctgctgacgc
     3661 aggcgctgcg cggttggggc gcgaacgtca cgccctacat ccccgaccgc ttcgaggaag
     3721 gctacggcct caacgcgggc gcgctcggca agctctctgc gcagggcgcg caggtcgtcg
     3781 tcacggtcga ttgcggcgca cgcgcgctcg acgaggtcgc gcacgccaat cggctcggcc
     3841 tcgacgtgat cgtgaccgat catcacgagc cggagggcga tcggctgccg gcggcccggg
     3901 cgttgatcaa ccccaagcag cccggctgcc cgtatcccga caagcacctg gccggcgtcg
     3961 gcctggcgtt tcgactggcg caggcgctgg cgtgggagct ggcccgcgac ggcagcagcc
     4021 tgccgtttcc gatcgacgac ctgctcgacc tggtggcgct gggcacggtc gccgatctgg
     4081 cgccgctcgt cggcgagaac cgggacatgg tgcggcgcgg actgcgattg atgaatgccg
     4141 ggtcccggcc cggggtggtg gcattgtgca acgtggccgg ccagcagccc ggcgagctgg
     4201 atgctggcag catcggctac tcgctcgggc cgcgcctgaa cgccgccggc cgcgtcgagc
     4261 atgcgcgcgc tgcataccag ttgctcgtca gcccgagcgt cgagcaggcg gcacagttcg
     4321 cctaccagct caacgagcag aaccgcgagc ggcagcgcct cacagccgag atggtcgatg
     4381 cggccgccgc ccaggcgctc gccggcgatc ccgaggcgcc gattctcttc gccgcgagcg
     4441 cgggcttctc gccgggcgtg atcgggctgg ctgccagccg cctggtcgaa gtgtatcacc
     4501 ggccggcgat cgtcatgagc gtcggcgagg gcgaggcgcg cggctcggcg cgcagcatcc
     4561 cgcagtttca catcacgcac gcgctggacg agtgcaagga cctgctcgtc aaacacggcg
     4621 gtcacgccgc cgccgccgga ttcagcgtcg cgctggattg catagacgag ctgaaagcgc
     4681 ggctcacctc gatcgccgca gcccggcaag ccgaggggga ttggtcgccc gtgctcaagg
     4741 cggatgcgct cgtgcgcctg tccacgttga cgtacgagat ggtcaggcat ctggcgcggt
     4801 tggagccgta cggcatgcag aatccgcagc ccgtcttcgt cagccgggat attcgcgtgc
     4861 gcgg
//
LOCUS       DBNHXB010000188         7092 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3901195, whole genome shotgun sequence.
ACCESSION   DBNHXB010000188 DBNHXB010000000
VERSION     DBNHXB010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3901195"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1570)
                     /locus_tag="AC162G_07575"
     CDS             complement(<1..1570)
                     /locus_tag="AC162G_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006448 - regulation of translational
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongator complex protein 3"
                     /protein_id="HIR5075708.1"
                     /translation="MNGRDIVDVTPYANAVMAIIQEVRARERVDADVLGAILRRYPRD
                     GRSMFSKNLLVRTYRHLCETGQLEFDEQTLLRLQMKPTRTISGVAPVTVLTKPYPCPG
                     KCIFCPTDVRMPKSYLPDEPGAMRAEQHGFDPYAQTKARIDTFDGNGHATDKIELLIL
                     GGTWSSYRRDYQEWFVRRCLDAMNGCASSSLAEAQALNETARHRNVGLVIETRPDHVT
                     PEEIRWLRYLGVTKVQMGAQSLDDRVLELNKRGHTVEDTRQAMTLLRAAGFKVVLHWM
                     PNLLGATPGSDLIDFQRLWDDEALRPDEIKIYPCSLLANAELYEVWLRGEYHPYPDDT
                     LVDLIAACKRRVPRYCRINRVYRDIPAPNIVAGSTLSNMREIVRRTMQARGEHCECIR
                     CREVRGRRIEDGVLHFEDSGYTTRASEEHFIAFNTPAGKLAGYARLSLPQKCVALGID
                     EIRGAALIREVHVYGPALAIGDAIEGRAQHRGLGTRLLAEAERIAAGRGYRRMAVIAA
                     LGTREYYAKRGYVRD"
     gene            1603..2376
                     /locus_tag="AC162G_07580"
     CDS             1603..2376
                     /locus_tag="AC162G_07580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5075709.1"
                     /translation="MKTAIALRLLRLNREFYDTFAADFADSRRALQPGIVRALDGLGT
                     FESVVDVGCGDGRVGLALAGGVAGHRVSRYLGVDYSEGLLRQASPRMPLGFRFMLCDL
                     TSVDWPNQVRRAEKARFEAAVCFSALHHIPGARRRLRLLRGIRSLLEPGARCAVSVWQ
                     FLHVPRLRRRIVPWSEVDLRPEDVDAGDYLLDWRRGGRGLRYVHHFDEPELTRLCHRA
                     GFRLLDVYRSDGHAPALRVAEGAGSGEMGLYILLQVVIY"
     gene            2412..3845
                     /gene="amrB"
                     /locus_tag="AC162G_07585"
     CDS             2412..3845
                     /gene="amrB"
                     /locus_tag="AC162G_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HIR5075710.1"
                     /translation="MPTARAGSHAIRPPAVAGMFYPADPIELTRMIDSYLAQATTSET
                     EPSILIAPHAGYVYSGPVAAFTFKALAGRDCDGVIVLGFNHAYSYEFDGASIWLDGAW
                     RTPLGDVPIDSALARSIHSAEDSFSTGRDAHTTEHSIEVMLPFLQRVLPGLSFVPVSI
                     GRPTLDNCRALADGLARAIEGKHIFVVASTDLSHYPPYESAVMVDRETVAAVLSLDPL
                     ALEAIVEDVRRRDVPNLHTRMCGQGPVLTAMMLARHIGAERAELLRYANSGDVPEGGR
                     DRVVGYAAIGFSKGKPPALTDADRAELLRIARATLVANAENKPWPELRVDSPALRQPR
                     ATFVTLRRKPSGELRGCLGEVFARSEVWESVRRITVLSAVDDPRFMPLQAHEIPDTHI
                     EISVLSPLRRAASPEEVVVGRHGVRVWRGGHVGLFLPQVPVEQGWDRETYLDVLCEMK
                     AGLPSDAWRKPDTQLYIFEAEVFEEHE"
     gene            3846..5081
                     /locus_tag="AC162G_07590"
     CDS             3846..5081
                     /locus_tag="AC162G_07590"
                     /inference="COORDINATES: protein motif:HMM:NF018434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5075711.1"
                     /translation="MIATEARKFLFTSAPLSGHLDWGGYLRTAAHLSGCGHDVLWVSE
                     EPVRAAVEAAGVRFRAVDAIGWRWPAAQLPADLKPGERAAARLQRALLVMLSEDAIVR
                     AVDNLLAVADEFEPDALIGEPTVGAAALAAEKLNVPYAVCGYPATRHDPETLGQAERA
                     VYQDAAARLKDLIARLDVRGRNWPGEFSPWPQSPDLHVVYWTREWYADESEALPQTHF
                     VGGVVSPPRGDAPGWFARLPPGMPLALVTLGSLFTDDPDFFLLAAHACTRAGVFPVIA
                     MGRSERAPNLKQAIAPRLPRCIAVAWADYDHLFPRLSVAVHHGGMGTTHAAVVHGVPQ
                     VIVPHAGDQSLQALRAEASGVGLMIRPKEATLDALQHAVETVARDPGFRIRARSLTAE
                     FAAMGGMTAAARHIERLVC"
     gene            5285..5629
                     /locus_tag="AC162G_07595"
     CDS             5285..5629
                     /locus_tag="AC162G_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003937585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR5075712.1"
                     /translation="MLKFLIRLVINAIALWLAVTLVEGLDFTGNVLGLLIVAIVFGLV
                     NALIKPIVQFVTMPISVITLGLFSLVINALMLLLTGWLTEPLEVSGFFPALWGSIIIS
                     IVSTVLNRLVGD"
     gene            complement(5861..6277)
                     /locus_tag="AC162G_07600"
     CDS             complement(5861..6277)
                     /locus_tag="AC162G_07600"
                     /inference="COORDINATES: protein motif:HMM:NF037170.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5075713.1"
                     /translation="MSVSTAHRPIMELRVAFTAREYERLVRFYCDGLGLEPAQLWPSD
                     QGRAVVLDMGQATLEIFDETQANTIDQIEAGQRLSGQIRFALKVPDLKAAMERLLSNG
                     AILVHPPVVTPWGDHNVRLQDPEGMQVTLFQVLEQS"
     gene            complement(6326..6709)
                     /locus_tag="AC162G_07605"
     CDS             complement(6326..6709)
                     /locus_tag="AC162G_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5075714.1"
                     /translation="MAQPDLIGIIVRDMAAALRFYTLLGLDIPSNLESEQHVEVSLPN
                     GFRIAWDSLEMIKSLDPDWTEPVGQRMTLAFKCGSPAEVDALYDRVVQSGYRGHKAPW
                     DAFWGQRYAVVVDPDGNLVDLFAGL"
     gene            complement(6816..>7092)
                     /locus_tag="AC162G_07610"
     CDS             complement(6816..>7092)
                     /locus_tag="AC162G_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075715.1"
                     /translation="CAAGQTMTHNNRLQRTALGFFGPGGVAEEYRGKGTGKALLLACL
                     LDMKLKGYGYAIIGDVGPAEFYMRTAGAVEIPNSTPGVYKTMLRRGE"
BASE COUNT         1159 a         2272 c         2330 g         1331 t
ORIGIN      
        1 cgtcgcgaac atatcctctt ttggcgtagt actcgcgcgt cccgagggcg gcgatgacgg
       61 ccatacgcct gtagccgcgc ccggcggcga tgcgctcggc ttcggccagc aaccgcgtgc
      121 cgagaccccg gtgctgggcg cggccttcta tcgcgtcgcc gatggcgagc gccgggccgt
      181 atacgtgcac ctcgcgaatt aacgccgcgc cccgtatctc gtcgatgccg agggcgacgc
      241 atttctgagg cagcgaaagc cgcgcgtaac ccgccagttt ccccgccggc gtgttgaagg
      301 cgatgaagtg ctcttcgctg gcgcgcgtcg tgtagcccga atcctcgaag tgcaggacgc
      361 catcctcgat ccttcgcccg cgcacttccc ggcagcggat gcactcgcaa tgctctccgc
      421 gcgcctgcat ggtgcggcgc acgatctcgc gcatgttcga cagcgtgctg cccgcgacga
      481 tgttcggcgc ggggatgtcg cggtacaccc ggttgatgcg gcaatagcgc ggcacgcgcc
      541 gcttgcaggc cgcgatcaga tcgacgagcg tatcgtccgg ataagggtga tactcgcctc
      601 gcagccagac ttcgtacagc tcggcgttcg ccaggagcga gcagggataa atcttgattt
      661 cgtcggggcg gagcgcctcg tcgtcccaca gtctctgaaa gtcgatcagg tcgctcccgg
      721 gcgtcgcgcc gagcaggttg ggcatccagt gcagcacgac cttgaatccg gccgcgcgca
      781 gcagggtcat cgcctgccgc gtgtcctcga cggtgtggcc gcgcttgttg agttcgagta
      841 cccggtcgtc gagcgactgc gcgcccatct gcaccttggt cacgccgagg tagcgcagcc
      901 agcgaatctc ctccggcgtg acgtgatcgg gccgcgtctc gatcaccagg ccgacgttgc
      961 gatggcgcgc cgtctcgttc agcgcctgcg cttccgccag cgacgacgaa gcgcagccgt
     1021 tcatcgcgtc gaggcagcgc cggacgaacc actcctggta gtcgcgccga tagctcgacc
     1081 acgtcccgcc gaggatgagg agctcgattt tgtcggtcgc gtggccgttg ccgtcgaatg
     1141 tgtcgatgcg cgctttcgtc tgggcgtacg ggtcgaagcc gtgctgctcg gcgcgcatcg
     1201 cgcccggctc gtccggcagg tagctcttgg gcatgcgcac atcggtgggg cagaagatac
     1261 acttgccggg gcacgggtag ggcttggtca gcaccgtcac cggcgccacg cccgagatcg
     1321 tgcgcgttgg cttcatttgc aggcgcagca gcgtctgctc gtcgaactcc aactgcccgg
     1381 tctcgcacag atgcctgtaa gtgcgcacca gcaggttttt gctgaacata ctgcgcccgt
     1441 cgcgggggta gcggcgcaga atcgcgccca gcacgtcggc gtcgacgcgt tctcgcgcgc
     1501 gcacttcctg gatgatcgcc atcacggcgt tggcgtacgg agtgacatct acgatgtcac
     1561 gcccgttcat ggctggcatg cttgatagta taatccagat cgatgaagac ggccatcgct
     1621 ctccggttgc tccgcctcaa ccgggagttc tacgatactt tcgcggctga cttcgccgat
     1681 tcgcgccgcg cgctgcagcc gggcatcgtc cgcgcgcttg acgggctggg gacgttcgag
     1741 tcggttgtcg acgtcggctg tggcgacggg cgagtgggac tggcgctggc aggtggggtt
     1801 gccggccacc gggtgagccg ctacctcggc gtcgactaca gcgagggtct cctgcggcag
     1861 gcatcaccgc gtatgcctct cggttttcgg ttcatgttgt gcgatctcac gagcgtcgat
     1921 tggccaaatc aggtgcgccg ggcggaaaaa gcccggttcg aagccgccgt gtgcttctcg
     1981 gcgctgcacc acattcccgg cgcgcgccgc aggttacgcc tgctgagggg aatccggtcg
     2041 ctcctggagc cgggcgcacg ctgcgccgtc tcggtctggc aattcctgca cgtgcctcgc
     2101 ctgcgacgca gaatcgtccc gtggtcggaa gtcgatctgc gcccggaaga tgtcgacgcg
     2161 ggagactacc tgctcgactg gcggcgcggc gggcggggcc tgcgttatgt gcaccatttt
     2221 gacgagcctg aattgacgag gctctgccac cgcgctggct tccgcctcct ggatgtctat
     2281 cgcagcgatg ggcacgcacc tgccctgagg gtagccgaag gggccggcag cggcgaaatg
     2341 ggactgtaca tcctcttgca agtggtgata tactgattgc atagggtggg ctggtgagag
     2401 ggaggtctct catgcctact gcaagagcag gctcgcatgc tattcggccg ccggccgtgg
     2461 ccggtatgtt ctatccggcc gatccaatcg aattgacccg catgatcgac agttacctgg
     2521 ctcaagcgac gacgagcgag actgagccgt cgattttgat cgcgccgcat gccggttacg
     2581 tgtacagcgg gccggtcgcc gcattcactt tcaaggcgct ggccggccgc gactgcgacg
     2641 gcgtgatcgt gctgggattc aatcacgcgt acagttacga attcgacggc gcgtcgatct
     2701 ggctcgacgg cgcgtggcgc acgccgctgg gcgacgtgcc gatcgattcc gcgctggccc
     2761 gctcgataca cagcgccgag gacagtttct ccaccgggcg agacgcgcac acgaccgagc
     2821 actcgatcga ggtcatgctg cctttcctcc agcgggtgtt gcccggcctg tctttcgtcc
     2881 cggtctcgat cggccgcccc acgctcgaca actgccgggc gctggccgac gggctggccc
     2941 gcgcgatcga gggcaagcat atcttcgtcg ttgcctcgac cgacctctcg cattatccgc
     3001 cgtatgagag cgcggtgatg gtcgatcgcg agacggtggc ggcggtgctc agtctcgatc
     3061 cgctcgcgct cgaagcaatc gtcgaggacg tccgccgccg cgacgtgccc aacctccaca
     3121 cgcgcatgtg cggccaggga cctgtgctga ccgcgatgat gctggcgcgc cacatcggcg
     3181 cggagcgagc tgagctgctg cgctacgcca attcgggcga cgtgcccgag ggcgggcgcg
     3241 accgggtggt cggctatgct gcgatcgggt tctcgaaggg caagccgccg gcgctcacgg
     3301 acgccgaccg cgccgagctg ctgcgcatcg cgcgcgcgac gctcgtcgca aacgccgaaa
     3361 acaagccatg gcccgagctg cgcgttgatt cgccggcgct gcgccagccg cgcgcgacgt
     3421 tcgtcacgct gcgccgcaag cccagcggcg agctgagagg ctgtctcggc gaagtgttcg
     3481 cgcggagcga agtgtgggag tcggtgcggc gcatcacagt actgtcggcg gtcgatgatc
     3541 cgaggttcat gccgctgcag gcgcacgaaa tccccgacac gcacatcgag atcagcgtgc
     3601 tgtcgccgct gcggcgggcc gcgtcgccgg aagaggtggt cgtcggcagg catggtgtgc
     3661 gggtgtggcg cggcgggcac gtcgggctat tcctgcccca ggtgccggtc gagcaggggt
     3721 gggatcggga gacgtacctc gacgtgttat gcgagatgaa ggcggggctg ccgagtgacg
     3781 cctggcgcaa gccggacacg cagctctaca tcttcgaggc cgaggtgttc gaggaacacg
     3841 agtagatgat agccacagag gcacggaagt tcctgttcac ctccgcgccg ctgtccggcc
     3901 acctggattg gggtggctat ctccggacgg cggcgcatct gtcaggctgc ggccacgacg
     3961 tgctgtgggt cagcgaagag ccagtgcgcg cggccgtgga ggcggcaggc gtgcgattcc
     4021 gcgccgtcga cgccataggc tggcgctggc cggcagcgca gcttcctgcg gacctgaagc
     4081 cgggcgagcg cgctgccgcc cggcttcaac gcgcgctcct cgtcatgctc tcggaagatg
     4141 cgatcgtgcg cgccgtggac aatctgctgg ccgttgcaga cgagttcgaa cccgacgcgc
     4201 tgatcgggga accgaccgtc ggcgcggcgg ctcttgcggc ggagaagctg aacgtgcctt
     4261 atgccgtgtg cggctatccg gccacccggc acgatcccga gaccttaggt caagccgagc
     4321 gcgcggtgta ccaggacgcc gccgcgcggc tgaaggatct gatcgcccgc ctcgacgtgc
     4381 gcggccgcaa ctggccgggc gagttctctc cctggccgca gtcgcccgat ctgcacgtcg
     4441 tgtactggac gcgcgagtgg tacgcggacg aaagcgaggc gctgccgcag acgcacttcg
     4501 tcggcggcgt ggtctcgccg ccgcgaggag atgcgcctgg ctggttcgcc cgactgccac
     4561 cgggcatgcc gcttgcgctc gtcacgctcg gcagcctgtt caccgacgac ccggacttct
     4621 tcctcctggc ggcgcacgcc tgcacgcgcg cgggcgtctt ccccgtcatc gccatggggc
     4681 gcagcgagcg ggcgccgaat ctcaagcagg cgatcgcgcc gcgcctgccg cgctgcatcg
     4741 ccgtcgcgtg ggcggactac gaccatctgt tcccgcgcct gtccgtcgcc gtgcatcacg
     4801 gtggaatggg cacgacgcac gcggccgtcg tgcacggcgt gccgcaggtg atcgttccgc
     4861 acgcagggga tcagagcctg caggcgctgc gggccgaggc gagcggcgtc gggctgatga
     4921 ttcgaccgaa agaggcgacg ctggatgcgc tgcagcacgc agtcgagacg gtcgcgcgcg
     4981 atccgggctt caggattcgg gcacggagcc tgacggcgga atttgcggcg atggggggca
     5041 tgacggcagc cgcacgtcac atcgagcgac tggtgtgctg atgtatacct tcagtgccga
     5101 tccgaaaacc ctcggagtgc ggggctttag cccgctttgg ccgcgcagac aaaagtctgc
     5161 actccacata cctgacttga caatgtcaga ttcggctcgt cgcgccgtcc tcggcgcggc
     5221 catcgccgcc ggggacggcg gcttgagcct aaatgtgata ttgtcgaact aaagaggagg
     5281 cgctgtgctg aaatttctca ttcgtctcgt catcaatgcc attgccctct ggctggccgt
     5341 gactctcgtc gagggactgg acttcaccgg caacgtcctt ggcctgctga ttgtggcgat
     5401 tgtctttggt ctcgtcaacg ctctgatcaa gcccatcgtt cagttcgtga cgatgccaat
     5461 cagcgtcatc acgctggggt tgttttcgct ggtcatcaac gcgctgatgc tgctgttgac
     5521 gggctggctg accgagccgc tggaagtgag cggattcttc ccggcgttgt ggggcagtat
     5581 aatcatcagc atcgtgagca cggtgctgaa ccggcttgtg ggcgattagg atttcagcac
     5641 ggagatacgg agggcgccgg gctttctcag gatgctccga gcgctctgcg tctctgcggc
     5701 gcgacccaat ccgcgatgcg gctgcacacg gtgttgggcg gttgctcaca tagcaccgct
     5761 caacgtcgcg gttcacccgc ttggccgagc acgaccggag ggagcgcgaa ggccaagtcg
     5821 ggtgcaaccg ctgggccgcc gcttcacgac aaggtctgcc tcatgattgc tcaagcacct
     5881 ggaatagcgt gacttgcatg ccctccgggt cctggagtcg tacgttgtga tctccccaag
     5941 gtgtaaccac gggcgggtgg accaggattg cgccgttcga gagcaagcgc tccatagctg
     6001 ccttcaagtc aggcaccttc aacgcgaacc gaatctgtcc gctgaggcgc tggcccgctt
     6061 ctatctggtc aatcgtattg gcctgtgttt cgtcgaaaat ctccagcgtg gcctggccca
     6121 tatcgaggac gacggcacga ccttgatcgc ttggccagag ttgcgctggt tcgagcccga
     6181 gaccgtcgca atagaatctc acgaggcgct catattcgcg ggcggtaaac gcgacccgca
     6241 attccatgat cgggcgatga gcagttgaca cagacatgat aggcaatgga cctttctttc
     6301 cggaaatcgc aattcacagc aaacgttata gtcctgcaaa cagatcaacg agattgccat
     6361 cgggatcgac cactacggca tagcgttgtc cccaaaaggc atcccacggc gccttgtgac
     6421 cccgataccc gctctgaacc acgcggtcat acaatgcgtc cacctcggca ggactcccgc
     6481 atttgaaagc taaagtcatc cgctggccga ctggttcagt ccagtcgggg tcaaggctct
     6541 taatcatctc cagactgtcc caggcaatac ggaacccgtt tggtaggctg acctcaacat
     6601 gttgttcgga ttccagattc gagggaatgt ccagtccgag cagagtatag aaccgcagtg
     6661 ccgcggccat gtcgcgcacg atgattccga tcaggtctgg ctgtgccatc taagtctccc
     6721 ccttccagga tcgtttttct gtgatcgcgg cgtgcgtggt gccgcccaac ggtcatcgtg
     6781 cgcgcgatgc gacagcacga gacgaaagac cgatcttatt caccacgccg tagcatggtc
     6841 ttgtacacgc ccggcgttga attcggaatc tcaactgccc ccgccgttct catgtagaac
     6901 tcggctgggc caacatcgcc gatgatggca tacccgtaac ccttcaactt catgtccaag
     6961 agacaagcca gcagcaacgc cttgcccgtg cccttgccgc gatactcttc ggcgacccca
     7021 cctggaccaa agaagcccag cgctgtccgc tgcaaccggt tgttatgcgt catcgtctgg
     7081 ccggctgcac aa
//
LOCUS       DBNHXB010000189        10495 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2056542, whole genome shotgun sequence.
ACCESSION   DBNHXB010000189 DBNHXB010000000
VERSION     DBNHXB010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10495)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10495)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10495
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2056542"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="AC162G_07615"
     CDS             complement(<1..1354)
                     /locus_tag="AC162G_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HIR5075716.1"
                     /translation="MTRVKRSALVMCLAALAVGFALLAIVRAPTTIAASEPEVTPAPR
                     LVVLRVYVRSLDDAARLTSGGWDVLEARGPDYLLVMGDDVVMRRLRAQGFDVAIDHVL
                     DAAAEISPFSYYGGYRSVAEHDQHMDGIAAAHPDLAVVVDYGDSWRKVNSRPDGHDLK
                     AICITKLRPGDCALDPEADKPRFLLIAAVHARELSTAELAWRWIDYLVDNYDVDPDVT
                     MLLDHNEMWIIPVANPDGRHIVEQGGNSPYLQRKNANDSLGNCSDPPTASNHHGVDLN
                     RNADFQYGGASTSIDPCAQTYRGTNPASEPEEDALENLMRSLFRDQRGAAITDTAPLT
                     TTGAMLTLHSYGNLVLLPWGWTECFLSCPPSLRAPNDAGLRSFAFRMSYYNGYDTGQG
                     SELLYAASGTTDDWAYGVLGIPGFTFEVGPQFFGCSGFTPQYSCQDGTFWPLQRGAFL
                     Y"
     gene            complement(1498..3210)
                     /locus_tag="AC162G_07620"
     CDS             complement(1498..3210)
                     /locus_tag="AC162G_07620"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR5075717.1"
                     /translation="MSTSLPSSADDLMQWTWPQIEPHYRDLAARPLNTANVGAWLVDW
                     SQLGEAVNEMYNRLWVATTVNTADEEAGRRFAAYLDEIFPAAQAAEHKLKERLLASGL
                     EPEGYEIPLRNMRTEAELFREANLPLLAEEKKLSTAFDEIVGAQTATWDGKEVTLQQL
                     QPVFLDPDRAKRERAWRLSMERRLADREKIDELWTQFLRVRTHIAENASFGHDYRAYR
                     WRQLLRFDYTPADCETFHRAIEEVAIPASLRIYERRRKQLGVSSLRPWDLNVDPLNRP
                     PLHPFETIDELRGKTSAIFHRVDQQLGAHFDTMAREGLLDLDNRKNKAGGGYCTEFAA
                     ARRPFIFMNAVGVHDDVQTLLHEGGHAFHVFESARLPYLQQKQVGLEFAEVASMGMEL
                     LAAPYLSANHGGFYSEADAARARIQHLEEAILFWPYMAVVDAFQHWVYQNPGAALDPA
                     QCDAQWAALWKRFMHGVDWSGLDDVLITGWQRKGHIHQDPLYYVEYGLAQLGAVQVWR
                     NALKDQAASVARYRRALSLGGTQPLRMLYEAAGAKFAFDASTLQEAVGLMEQAIAALD
                     PEQG"
     gene            complement(3224..3541)
                     /locus_tag="AC162G_07625"
     CDS             complement(3224..3541)
                     /locus_tag="AC162G_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5075718.1"
                     /translation="MNKSEPRVTPWPNAEPPTESAIRQLFDAEGLRPYAWSNGPGDVY
                     GAHHHAYHKVIYVVEGSITFGLPDEGRQVTLNMGDRLDLPAGVVHDAVVGLQGVVCLE
                     AHH"
     gene            complement(3754..4167)
                     /locus_tag="AC162G_07630"
     CDS             complement(3754..4167)
                     /locus_tag="AC162G_07630"
                     /inference="COORDINATES: protein motif:HMM:NF017020.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR5075719.1"
                     /translation="MSGQPRRPVETPREWLRYAEQDFGVAGREMLSAEPAFHTVCFLC
                     QSAAEKFLKGFLIAQGWSLEKTHDIVALLGLCAEYDVGLGAMMAEGAVLNEYIVAGRY
                     PGDIAFEDMGRAQAEEALNAARHIRARVMALMGAE"
     gene            complement(4151..4525)
                     /locus_tag="AC162G_07635"
     CDS             complement(4151..4525)
                     /locus_tag="AC162G_07635"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR5075720.1"
                     /translation="MPEQTHTLQWDTRLQTSQITPELVAYIVEKIVRSIAPQRIILFG
                     SRARGAATDTSDLDLFIVQDSYTSNREVRRQIESLLWGRRFGVDLIVRRPEEVARNVA
                     DGNPFYTRHILGEGKVLYERSA"
     gene            complement(4593..4988)
                     /locus_tag="AC162G_07640"
     CDS             complement(4593..4988)
                     /locus_tag="AC162G_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR5075721.1"
                     /translation="MIFVDTSAFLAVLNADDQNHADARRVWENLVTREDVLVSSNYVL
                     VETFAVVQNRLGMAAVKLFQDDILPVVTVEWVDETLHQAGIAALLTANRRQLSLVDCI
                     SFEMMRRLGIKTAFTHDRDFAEQGFECIP"
     gene            complement(4985..5236)
                     /locus_tag="AC162G_07645"
     CDS             complement(4985..5236)
                     /locus_tag="AC162G_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HIR5075722.1"
                     /translation="MLRTQIQLTEEQSRALKMLAAKQGKSVSELIRQSVDRLIQSAGA
                     VDMADRRRRALAAAGQFRSGQTDLAVEHDRYLAEDYQAR"
     gene            complement(5311..8157)
                     /locus_tag="AC162G_07650"
     CDS             complement(5311..8157)
                     /locus_tag="AC162G_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5075723.1"
                     /translation="MADPAPHNKPAVIRTPDQRLRVFVSSTLQELADERDAAREAIAR
                     LRLAPVMFELGARPHPPRELYRAYLDQSHIFIGIYWQRYGWIAPGETISGLEDEYRLS
                     GAKPKLIYIKKLPEPVEGSPAPDREPRLKELLDRIRNDDTASYKPFASAHELRELIEN
                     DLALLLTERFETSQADAAEPAPPRSNLPAPSTRFIGRESEMAEVKRLLSNTRLLTLTG
                     AGGTGKTRLSLQVAADLLDSFADGVWLVELAPLSDPVLIPQSVATTLGVREVPGRPLM
                     VALTDYLRAKHLLLILDNCEHLIDACAHLADHLLRACAHLKILASSREALGIAGEIAF
                     RVPSLAVPPDVLSAARDLEGLTRCESAQLFVERTMAVQPHFAVTPQNAPAIAQICQRL
                     DGIPLALELAAARVKVLSVEQIAARLNDRFRLLTGGSRAALPRQQTLRASIDWSYSLL
                     PETEQTLFRRLAVFVGGWTLEAAESVCSDQLSVSSEPKPLTPDHSPLFTEDVLDLLTH
                     LVDKSLVIKDERADEVRFHRLETIRQYARDKLLESGEAARARDRHLEFFFKFAQEAER
                     DSGGAETLTWFNRLETEHDNLRAALEWALESNPDTAIELAVCLTGFWGRRGYATEGSK
                     WLGEALARVEALPPPDSVAAHRRLVARAKALVYWGNMALYLGDPMLGRARFEEGVTLS
                     RQTGDEWLIAIALQRLADAELFTGEPQDAKAAIEESVALFRKVGDRKGLAFALYLLAR
                     IAHMRGDRETSRASLEESTRLSRESDLPWESAMSLFGLGLTARSLGDYAEARARFREC
                     LALFREMGDKHFTNVLRSELAETERLEGNYEEAVPLYNETILTWRDFGHRGGIARCLE
                     VFAFIANARSQKQSSVESTGYLKRAARLLGAAEALRKISQSAMPSDEFVEYDRELSAL
                     EARLDRATFDAAWAEGRAMSMEQAIEYALETTDD"
     gene            complement(8418..10352)
                     /locus_tag="AC162G_07655"
     CDS             complement(8418..10352)
                     /locus_tag="AC162G_07655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5075724.1"
                     /translation="MSTPQTAPCGSWKSPITSDLIVAETIWPAQIQLDGDDVYWMEVR
                     PGEGGRYVIVRRSPDGRTVDVTPPPFNARTRVHEYGGLAYLVVDGTAYFPNFDDQRLY
                     RQKPSSDPQSITPSSNLRYADGVIDRRRNRIICVREDHTVSDREAVNTIVAIRLDGDE
                     DAGAVLVSGNDFYSSPRLSPDGSSLAWLTWNHPNMPWDGTELWVAGVNADGSLGRAER
                     VAGGLDESIFQPEWSPDGVLHFVSDRTGWWNLYRLRERQAEPLCPMEADFGMPQWVFG
                     MSIYAFESAERIVCRYIERGVSRLASLDTRTLKLEPIDTLYTDIARVQAQPGHALFIG
                     GSPTEPLSVVRLDLATRQHEVLRRSDKVTVDAGYLSQPLAIEFPTENDLTAHAFFYPP
                     HNRDVVAPSGEKPPLLVMSHGGPTAAVSTTLNLGIQYWTSRGIAVLDVNYGGSTGYGR
                     AYRERLKGQWGVVDVDDCVNAARYLVERGDVDGNRVAIRGGSAGGYTTLAALTFRDFF
                     KAGASHFGVSDLEALAKDTHKFESRYLDGLVGPYPERRDLYVERSPIHFTHNLSCALI
                     LFQGLEDAVVPPDQSEKMFEAVRAKELPVAYLPFEGEQHGFRRAENIKRTLDAELYFY
                     SKVFGFELADPVEPVHIENL"
BASE COUNT         1950 a         3320 c         3212 g         2013 t
ORIGIN      
        1 catacagaaa cgcgccgcgc tgcaagggcc aaaatgtgcc gtcctggcac gaatactgcg
       61 gcgtgaatcc gctgcatccg aagaactgcg gcccaacctc gaaggtgaag cccggaatgc
      121 ccaacacgcc gtaggcccag tcgtcggtcg tcccgctggc ggcatagagc agctcggatc
      181 cctggccagt gtcgtagccg ttgtagtagc tcatacggaa cgcgaacgag cgcaagcctg
      241 catcgttcgg cgctcgcagg ctgggcgggc acgacagaaa gcactcggtc cagccccacg
      301 gcagcaagac caggttgccg taactgtgca gcgtgagcat cgcgccggtc gtcgtgagcg
      361 gcgccgtgtc ggtgatggcc gcgccgcgct gatcgcggaa aaggctgcgc atcaagttct
      421 cgagcgcatc ttcctccggc tcggatgcgg ggtttgtgcc ccggtaagtc tgggcacatg
      481 gatcgatgct tgtgctcgcg ccgccgtatt gaaagtcggc gttgcggttg aggtcgacgc
      541 cgtgatgatt tgaggcggtg ggtggatcgc tgcaattgcc gagcgagtcg ttggcgttct
      601 tgcgctgcag ataaggcgaa ttgccgccct gctcgacgat gtgccggccg tcgggattgg
      661 cgaccgggat gatccacatc tcgttgtggt cgagcagcat ggtgacgtcg ggatcgacgt
      721 cgtagttatc gacgaggtag tctatccagc gccaggctag ctcggccgtg ctcagctcgc
      781 gcgcgtgaac cgccgcgatc agcaggaagc gcggcttgtc tgcctccgga tcgagcgcgc
      841 aatcccctgg gcgcagcttg gtgatgcata tcgccttgag atcgtggccg tccggccggc
      901 tgttgacctt gcgccacgag tcgccgtaat cgacgacgac ggccaggtca gggtgcgcgg
      961 cagcgatgcc gtccatgtgc tggtcatgct cggccacgct gcggtatccg ccgtagtagg
     1021 agaacgggct gatctccgca gcggcgtcga ggacgtggtc gatcgcgacg tcgaagccct
     1081 gcgcgcgaag ccggcgcatc acgacgtcgt cgcccatgac gagcaagtag tccgggcctc
     1141 gcgcctccag cacgtcccag ccgccggatg tgagccgggc ggcgtcgtcc aggctacgga
     1201 cgtagacgcg cagcacgacc aggcgaggcg caggcgtgac ttcaggctca gaggcggcaa
     1261 ttgtcgtggg cgcgcgcacg atcgccagca aagcaaaccc cacggcgagc gcggcgagac
     1321 acatcacgag cgccgagcgc ttaacgcgcg tcatagtgca acctcccgcc gtaaccaagg
     1381 cttagccgcg gatttgcgcg gatggacgcg gataaggtca aaatcagcgt agcccgcgtt
     1441 catccgcgtc ctagaaattt gtgctcgtgg taatttctca ggggcaacac catggcgtca
     1501 gccctgctcc ggatcgagtg cggcgatggc ctgttccatc aggccgaccg cttcctgcag
     1561 tgtgctggcg tcgaaggcga acttcgcgcc ggccgcttcg tagagcatcc ggagtggctg
     1621 cgttccgccg agcgacagcg cgcgccggta ccgggcgacc gacgcggctt gatccttcag
     1681 cgcattgcgc cacacttgca cggcgccgag ctgcgccagg ccatattcga cgtagtagag
     1741 gggatcctgg tggatgtgtc ccttgcgctg ccagccggtg atcaggacgt cgtccagccc
     1801 gctccaatcg acgccgtgca tgaagcgctt ccacagcgcc gcccactgcg cgtcgcactg
     1861 cgccggatcg agcgcagccc cagggttttg gtacacccag tgctggaaag catcgacgac
     1921 ggccatatac ggccagaaca ggatggcctc ctctaagtgc tggatgcgcg ctcgcgcggc
     1981 gtcggcttca ctatagaagc cgccgtgatt cgcgctcagg tacggcgccg ccaacagctc
     2041 catgcccatc gacgcgactt cggcgaactc cagcccgacc tgcttctgct ggaggtacgg
     2101 caggcgcgcc gactcgaaca cgtggaaagc gtgcccccct tcgtgcagca gcgtctgcac
     2161 gtcgtcgtgc acgccaacgg cattcatgaa gatgaagggg cgccgggctg ccgcgaactc
     2221 ggtgcagtag ccaccgccgg ctttgttctt gcggttgtcg agatcgagca gaccctcgcg
     2281 cgccatcgtg tcgaagtgcg cgccgagctg ctggtcgacg cggtggaaga tggccgaggt
     2341 tttgccgcga agctcgtcga tcgtctcgaa cgggtgcagc ggcgggcggt tcagcggatc
     2401 gacgttgagg tcccacggcc gaagcgaact gacgcccagc tgcttgcgcc gcctctcata
     2461 gatgcgcagg ctggcgggaa tcgcgacttc ctcgattgcg cggtggaacg tctcgcagtc
     2521 ggcgggcgtg taatcgaagc gcagcaactg cctccagcga taggcgcggt agtcgtggcc
     2581 gaagctggcg ttctccgcga tgtgtgtacg cacgcgcagg aattgagtcc acaattcgtc
     2641 gattttctcg cggtcggcca ggcgacgctc catcgacagg cgccacgcgc gctcgcgctt
     2701 cgcgcgatcc ggatcgagga agacaggctg caattgctgc agagtgacct ctttgccatc
     2761 ccacgtggcc gtctgcgcgc cgacgatttc gtcgaacgcc gtggacagct tcttctcctc
     2821 ggccagcagg gggaggttcg cctcgcggaa cagctctgcc tcggtgcgca tattgcgcag
     2881 aggaatctcg tagccttccg gttcgaggcc ggacgcgagc agtctctcct tgagcttgtg
     2941 ctcggcggcc tgcgcggcgg gaaagatctc gtcgaggtag gcggcgaagc gacgcccggc
     3001 ctcttcgtcc gccgtgttga cggtcgtcgc cacccataag cggttataca tttcgttgac
     3061 ggcttctccg agctgcgacc agtcgaccag ccacgcgccg acgttcgcag tgttcagcgg
     3121 gcgcgcggcg aggtctcgat agtgtggctc gatttgcggc caggtccact gcatcaggtc
     3181 gtcggcgctg ctcggcagtg atgtagacat aattggtcct ttgtcagtga tgtgcctcca
     3241 agcataccac accctgcagt ccgacgacgg catcgtgaac gacacctgcc ggcaggtcca
     3301 gccgatcgcc catgttcagc gtcacctgcc ggccctcgtc cggcaggccg aacgtgattg
     3361 agccttcgac gacgtagatg accttgtgat aggcgtggtg atgcgcgccg tacacatcgc
     3421 cggggccatt tgaccaggcg tagggtcgca gcccttcggc gtcgaacaac tgacggatgg
     3481 cggattcggt cggcggctct gcgttgggcc aaggagtgac gcgaggttca cttttgttca
     3541 tttcgttgat tcgttgtcct attcgttgac accggtcttc agtgcatact gaatcgcctg
     3601 ttccagcgtc atcccccgtc cgctggccca cgccgccgca aatgtggccc ttcgaggtag
     3661 gtgcgggcgg atggcgcagc gcccatcccg ctgtgactga gttccgcgtc gagacgatag
     3721 cgattttgaa gtagcacgcc gatcaagtgg tatctactcc gcccccatta atgccatcac
     3781 gcgcgcacga atgtgtcgag ccgcgtttaa cgcctcttcc gcctgcgccc gccccatgtc
     3841 ctcgaaggcg atgtcgcccg ggtaccgccc tgcgacgata tactcgttca ataccgcgcc
     3901 ctcggccatc atcgccccga gacccacatc atattccgcg cacagcccga gcaatgcaac
     3961 tatgtcgtgc gtcttctcca gcgaccaccc ctgcgcgatc aagaagccct tcaagaattt
     4021 ttcggccgcg ctctggcaca ggaagcaaac cgtatggaaa gcgggttctg cgctcagcat
     4081 ctctcgtccg gccacgccaa aatcctgttc ggcgtagcgc agccactcgc gcggggtctc
     4141 gaccgggcgt ctaggctgac cgctcataaa gcaccttccc ttcgcccaag atgtgccttg
     4201 tgtagaacgg attgccatcg gccacgttcc gggcgacttc ttcgggccgt cgcacaatca
     4261 ggtcaacccc aaaacgtcgt ccccagagca agctttcgat ttggcgccgc acctcgcggt
     4321 tggaggtgta gctatcttgg acgatgaaga gatcgagatc gctcgtgtct gtcgctgctc
     4381 ctcgcgcgcg cgagccaaac aggataatcc gttggggtgc gatgctgcgc acgatcttct
     4441 ccacaatgta agcaaccagt tcgggagtta tctggctggt ttgtaaacgc gtatcccatt
     4501 gaagggtgtg tgtctgctcc ggcatcttgt gtgctccctg cttggatatg atgccaaccc
     4561 tgagggtgct cctgcccgat aaggaggcct gatcacggaa tgcactcgaa gccttgttcg
     4621 gcgaagtccc gatcgtgcgt gaaagcggtc ttgatcccca atcgccgcat catctcaaaa
     4681 ctgatgcagt ccaccaggct gagttgccgc cgatttgccg tgagcagcgc ggcaataccg
     4741 gcctggtgaa gcgtctcgtc tacccactcg accgtcacta ccggcaaaat atcgtcctga
     4801 aagagcttga cggccgccat tcccaggcga ttctgcacca ctgcgaaagt ttccaccagc
     4861 acgtagttgc tgctgaccaa cacatcttcg cgagtaacga ggttttccca tacgcgccga
     4921 gcgtctgcgt ggttctggtc gtcagcgttc aacacggcta gaaaggcgga ggtatccacg
     4981 aagatcatcg cgcctgatag tcctctgcca gatagcgatc atgctcaacc gccagatcgg
     5041 tctgccccga gcggaactgg ccggcggcag caagcgcgcg ccgccgacgg tcggccatat
     5101 cgacggctcc ggccgactgg atcagtcggt caacactttg ccggattaac tctgagaccg
     5161 acttgccctg tttggcggcc agcatcttca atgcgcggga ttgctcttca gtcagttgta
     5221 tctgggttct aagcatttct tcctctcata tcatgattac atttctgata aaatgataac
     5281 gcattattcg cgcttgtcaa ccatcgtttg tcaatcgtct gtcgtctcca gcgcatactc
     5341 gatcgcctgc tccatcgaca ttgcgcggcc ttccgcccag gccgcgtcga aagtcgcacg
     5401 atcaagccgg gcctccagcg cagacaactc gcgatcgtat tcgacgaact cgtcagacgg
     5461 catagcagac tggcttattt tgcgcagcgc ttccgccgcg cccagcaaac gcgcggcccg
     5521 cttcaagtac ccagtcgatt caactgaaga ttgtttctgg gagcgtgcat tcgcgataaa
     5581 cgcgaagacc tccagacacc gcgcgatccc gccgcgatga ccaaagtcgc gccaggtcag
     5641 aatggtttcg ttgtagagcg gcactgcttc ttcatagttg ccctcaagcc gctcggtctc
     5701 tgccagctca ctccgcaaga cattggtgaa gtgtttgtcg cccatttctc ggaacaatgc
     5761 caagcactcg cgaaaccgcg cgcgcgcctc ggcataatcg cctagagagc gtgccgtcag
     5821 tcccaaaccg aacagcgaca tcgccgattc ccaaggcagg tctgactcgc gcgataggcg
     5881 cgtgctctcc tccaaagagg cgcgcgacgt ctcacgatcg ccgcgcatat gtgctatgcg
     5941 cgcgagtaga tagagagcga acgcgagacc tttacgatcg ccaactttac gaaagagagc
     6001 cacgctttcc tcaatggcgg cttttgcgtc ttgaggctcg cctgtgaata gttctgcatc
     6061 cgccagacgc tgcagcgcga tcgcaatgag ccattcatct ccggtctgcc gcgacaaagt
     6121 tacaccctct tcgaatctcg cacgaccaag catcgggtct cccagataaa gcgccatgtt
     6181 tccccaatag acgagcgcct tcgctcgcgc gaccaggcgt cgatgtgccg ccacgctatc
     6241 cggcggcggc aatgcctcga cgcgtgccaa tgcttcgcca agccacttgc tcccttcggt
     6301 cgcgtaaccg cgcctgcccc agaatcctgt taaacacacg gctaactcga tcgccgtgtc
     6361 cgggttgctc tccagcgccc actccagcgc cgcgcgcagg ttgtcgtgct cggtctcgag
     6421 tcgattgaac catgtcaagg tttctgcgcc acccgaatcc cgctcggcct cttgcgcaaa
     6481 cttgaagaag aattccagat gacgatcgcg cgctcgcgct gcttcgccgg actccaacaa
     6541 cttgtcgcgc gcgtattggc gaatcgtctc caatcgatga aagcgcactt cgtcagcccg
     6601 ctcgtctttg atcaccagcg atttgtccac cagatgggtg agcaggtcga gcacatcttc
     6661 agtgaacagt ggagagtgat ccggtgtcag tggtttcggt tcactgctga ctgataactg
     6721 atcactgcat actgattcgg cagcctccaa ggtccagcca ccgacaaaga cagccagccg
     6781 ccggaacagc gtttgctctg tctccggcag caggctgtaa ctccaatcga tcgatgcacg
     6841 cagcgtctgt tggcgaggca atgcggcgcg gctgccgccg gtcagcaagc gaaagcgatc
     6901 gttgagccgc gcggcaattt gctcgacgct aaggaccttg acacgcgcgg cggcgagttc
     6961 gagtgcgagc ggaatgccgt cgagccgctg gcagatttgg gcgatggccg gcgcgttttg
     7021 tggcgtcacc gcaaaatgcg gctgcacggc cattgtgcgc tcgacgaaga gctgcgcgga
     7081 ttcgcatcgg gttaaacctt cgaggtcacg cgccgcgctt aggacatctg gcggcacagc
     7141 cagcgatggc acgcgaaaag caatctcgcc cgcaattccg agcgcctcac ggctgctggc
     7201 gagaattttc aggtgagcgc aggcgcgcag caggtgatcg gcgaggtgtg cgcacgcgtc
     7261 aatcaagtgc tcacagttgt ccagaatcag gagtagatgc ttggcgcgca ggtaatcggt
     7321 gagcgcgacc atcagagggc gacctggcac ttcacgcacg ccgagcgtgg ttgcgacgct
     7381 ctgcggaatc agcacggggt cggacaacgg cgcgagctcg acgagccaca caccgtcggc
     7441 aaatgaatcc agcaaatcgg cggccacctg gagcgagaga cgcgtcttgc ccgtgccgcc
     7501 tgcgcccgtg agcgtcagca gccgcgtgtt gcttaaaagc cgcttgacct cggccatctc
     7561 ggactcgcgg ccgatgaagc gggtcgacgg cgcgggcaga ttgctgcgcg ggggcgcggg
     7621 ttcagccgca tccgcttgag acgtctcgaa tcgctcggtc aacagcaacg ccagatcatt
     7681 ctcgatcaat tcgcgcagct catgggccga agcgaatggc ttgtaagatg ccgtatcgtc
     7741 gttgcggatc cgatcgagca gttctttcag gcgcggttca cgatccggcg cgggcgaccc
     7801 ttcgacaggc tcagggagct tcttgatata gatcaacttc ggctttgcac cggacagtcg
     7861 atactcatct tccagaccgg agatcgtttc gccgggcgcg atccagccgt agcgctgcca
     7921 gtagatgccg atgaaaatgt ggctttgatc gagataggca cgatacaact cgcgcggcgg
     7981 atgcgggcgc gcgcccagct caaacatcac cggcgcgagg cgcaaacgcg cgatcgcctc
     8041 acgcgcggca tcgcgttcgt cagccagttc ctgcagcgtc gaactcacga acacgcgcag
     8101 gcgctggtcg ggtgtgcgaa tgaccgccgg tttgttgtga ggcgctggat cagccatgcg
     8161 ttcctctcaa acatgctagt ataggcgggc gtaaatgagc cgacagttta gacataaggg
     8221 gtcaagcccc aatgcggctc aaagcgggtc tgtgacccgc gtcttgcccg gtgtcagccg
     8281 ctggatcaca gacccagcgg gagcgggaaa ctgacgtgaa ctagccgttg actcaaaaat
     8341 gatctcgttc tagacctggc aggttttctg gcgccggcag gctcagcccg atcggtgtgt
     8401 gaaaacctgc caggtcttca cagattctcg atatgcaccg gctccaccgg atcggccagc
     8461 tcgaagccga acactttcga atagaagtac agctcggcgt cgagggtgcg tttgatgttt
     8521 tcggcgcggc ggaagccgtg ctgctcgccc tcgaaaggca ggtacgccac cggcagctcc
     8581 ttcgctcgaa ccgcctcgaa catcttctcg gactgatccg gcgggacgac ggcgtcttcc
     8641 agcccctgaa agaggatcag cgcgcatgac aggttatgcg tgaaatggat cggcgagcgc
     8701 tcgacgtaca ggtcgcgccg ctcgggatac gggccgacca ggccatccag gtagcgcgac
     8761 tcgaacttgt gggtgtcctt cgccagcgct tcgagatcgc tcacgccgaa atggctcgcg
     8821 ccggccttga agaagtcgcg gaaggtgagc gcggccagtg ttgtgtagcc acctgcgctg
     8881 ccgccgcgaa tggcgacccg atttccatcc acgtcgccgc gctcgacgag atagcgcgcc
     8941 gcgttcacgc agtcgtcgac gtccacgacg ccccactgcc ccttgagccg ctcgcgatac
     9001 gcgcgcccgt agcccgtgct gccgccatag ttgacgtcca gcacggcgat gccccggctc
     9061 gtccaatact gaatccccag gttgagcgtc gtcgaaacgg cggccgtcgg cccgccatgg
     9121 ctcatgacca gcagcggcgg cttctcgccg gacggagcga cgacatcacg gttgtgcgga
     9181 ggatagaaaa aagcgtgcgc ggtgaggtcg ttctcggtcg ggaattcgat cgccagcggc
     9241 tgggagagat aaccggcatc gaccgtgacc ttgtccgacc ggcgcagcac ttcgtgctgt
     9301 cgtgtggcga gatcgagccg gacgaccgac aacggttcgg tgggcgaacc gccgatgaac
     9361 aaggcgtgtc ccggctgcgc ctgcactcgc gcgatgtcag tgtacagggt gtcgatcggc
     9421 tcgagcttga gcgtcctcgt gtcgaggctg gccagcctcg acacgccgcg ctcgatgtac
     9481 ctgcacacga tgcgctcggc cgactcgaat gcataaatgg acatgccgaa cacccactgc
     9541 ggcattccga aatcggcctc catcgggcac agcggctcgg cctggcgctc gcgcaggcga
     9601 tagaggttcc accagccggt acggtcggag acgaaatgca atacgccgtc cggcgaccac
     9661 tctggctgaa agatggactc atcaagcccg cccgccacac gctccgcccg acccaaggag
     9721 ccatcggcgt ttacgccggc gacccacagc tcggttccat cccagggcat gttcggatgg
     9781 ttccacgtca gccacgccag gctcgacccg tccgggctga ggcgcggcga cgagtagaag
     9841 tcgttgcccg agacgagcac cgcgccggcg tcctcgtcgc cgtccagccg gatcgcgaca
     9901 atcgtattca ctgcttcgcg atcggaaaca gtgtgatcct ctcgcacaca gatgatgcgg
     9961 ttcctgcggc gatcgatgac gccatcggcg taacgcaaat tcgaggaggg cgtgatcgac
    10021 tgcggatcgg agctcggctt ctgccggtag aggcgctgat cgtcgaagtt ggggaaatag
    10081 gccgtgccgt cgacgacgag atacgccagg ccgccgtatt cgtggacgcg ggtgcgggca
    10141 ttaaaaggag gcggcgtcac gtcgacggtc cgcccatctg gcgagcgacg cacgatcacg
    10201 tagcgcccgc cctccccagg gcgcacctcc atccagtaca cgtcgtcgcc gtcgagctgg
    10261 atctgtgcag gccagatcgt ctcggcgaca atcaggtcgg acgtgatcgg cgatttccac
    10321 gatccacagg gtgctgtttg cggtgtggac atggtattat cccccttggc taggttcctt
    10381 aattgcggtt tccagcactg cactatccaa tcatacctca gatcctgcac gttctccaga
    10441 gacggctctg tactgcgcgc gggcacaaat tgcgcttttc gcagggcgcg tttcc
//
LOCUS       DBNHXB010000190         9403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_881655, whole genome shotgun sequence.
ACCESSION   DBNHXB010000190 DBNHXB010000000
VERSION     DBNHXB010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9403
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_881655"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="AC162G_07660"
     CDS             <1..186
                     /locus_tag="AC162G_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR5075725.1"
                     /translation="VFTLNANLAGICGISLPCGFDAQGLPIGLQINGPAFGEEKMLRV
                     AYAYEQATEWHRRRPAL"
     gene            183..569
                     /locus_tag="AC162G_07665"
     CDS             183..569
                     /locus_tag="AC162G_07665"
                     /inference="COORDINATES: protein motif:HMM:NF017020.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR5075726.1"
                     /translation="MSDELDVGGWIQYAQADRHAAQSLFASGDYAVCAEMCQQMLEKI
                     IKALIVSQTGERPPYEHNLKRLADMVVGIAIPSRIVDILLNASPHYRFARYPGFGDLS
                     LYTAEYTRNLLDEAEEAYGWFLQQIK"
     gene            545..949
                     /locus_tag="AC162G_07670"
     CDS             545..949
                     /locus_tag="AC162G_07670"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR5075727.1"
                     /translation="MVLTADQVRTQILRFVEALQQRMRVERAVLFGSYAYGKPHEGSD
                     IDLAIVSPDFAAMNRLERLVFLERIAWDADAHYIEPVGFTAEELQNASRTSVLSEIRD
                     RGVVIRLDEASVEPLVVRDDRADYSVEEDGDA"
     gene            946..1932
                     /locus_tag="AC162G_07675"
     CDS             946..1932
                     /locus_tag="AC162G_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR5075728.1"
                     /translation="MKVIIPLGGLGTRLRPHTYTRPKPLINVAGKPMLGHIVDKFAGL
                     PVEEFIFVVGYLGEQIEHYVKSTYAFPARFVEQKEALGQAHALWLCREHIAGPVFVIF
                     GDTLFDADLSELSRTPQDNLAYVKEVEDPRRFGVAALQPDGGVERFVEKPNTMANRLA
                     VIGMYYYTDGSQLMSACKELMDRRIQTKGEYFLADANNLMIGQGARFVTRTVDVWLDT
                     GKPETVLETNRYLLDNGSDNSGQVHFPACVIVPPVNVHPTAKIEEAVIGPYATIGENC
                     RIRRAIVRDSIIEADATITDTFLDQSLIGREAMVTGRYRKFNVGDSASVGFS"
     gene            1959..2195
                     /locus_tag="AC162G_07680"
     CDS             1959..2195
                     /locus_tag="AC162G_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075729.1"
                     /translation="MDTISVHVECYSSHRYAQEPRAIVWRGFRVPIAAIENMWRTPDG
                     PLFRVRLDDGAIVDLQYVEASDEWLLTDRLLPRR"
     gene            2206..3390
                     /locus_tag="AC162G_07685"
     CDS             2206..3390
                     /locus_tag="AC162G_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5075730.1"
                     /translation="MRNTISQRVASVPPSGIRRFFDIAATMKDVISLGIGEPDFTTPQ
                     NILDAGVASLHKGETHYTSNSGTIELRRALSDHLRRLYGVRYDPESELLITVGVSEAL
                     YLALTAILDPGDEVIVPQPCFVAYTAEVVFAGGVPVPIATKVENNFQVTGAEIAAKIT
                     PKTKAILIGYPNNPTGAVMSRERLMEIAQLAEQHDLLVVSDEIYDRLVYAPMDGDGMG
                     AQHTCFPSLPGMQERTLLLGGFSKAYAMTGWRIGYAAAPAELSAAMRKVHQYTIMSAP
                     TTAQTAALEALQRGEEHVEAMRQEYDRRRRLIVRGLNEIGLKTFEPQGAFYAFPSVGV
                     SGMSDEEFVERLLNEEQVAVVPGNAFGAGGAGFVRCSYATAYEKIEQALERMHRFLRR
                     HG"
     gene            3466..4260
                     /locus_tag="AC162G_07690"
     CDS             3466..4260
                     /locus_tag="AC162G_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HIR5075731.1"
                     /translation="MNDLRDMEIARSQHWYRIPVRSVAKLLKERWPPQWLAFYQTKEF
                     GDKAHAVNYFSQVIDIREVFRWQLFPNQPMDEKGRQRYYQLILSPLQRLPQPIPSRRW
                     RLIVFIPTTWEKLMNAVEINDLYDESPLEDRLWAEFKRLKIAAERQEYVQVGDHRYAL
                     DFAIYCATGKIDVETDGDTWHASSEKSQLDNIRDNDLKTLGWRTFHFTTHQIQEQAAE
                     YCISTIVENINRLGGLEEGRLAPRKINLDGLEQLTLPDPEVHDDPD"
     gene            4296..4703
                     /gene="mntA"
                     /locus_tag="AC162G_07695"
     CDS             4296..4703
                     /gene="mntA"
                     /locus_tag="AC162G_07695"
                     /inference="COORDINATES: protein motif:HMM:NF047752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII toxin-antitoxin system MntA family
                     adenylyltransferase antitoxin"
                     /protein_id="HIR5075732.1"
                     /translation="MRIPSKAQLEPLARRYGLRLIVLFGSQVTGHTHPESDVDVAVWA
                     ARPITPGRRDRLWRELTDLFQTEIDLALLNHAEPLLMKHVARTGRLLYEGRRWAWEEF
                     KGFAFRYYLDTRKFRDDLARHLDREIRRPRHAG"
     gene            4693..5151
                     /gene="hepT"
                     /locus_tag="AC162G_07700"
     CDS             4693..5151
                     /gene="hepT"
                     /locus_tag="AC162G_07700"
                     /inference="COORDINATES: protein motif:HMM:NF047751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII toxin-antitoxin system HepT family
                     RNase toxin"
                     /protein_id="HIR5075733.1"
                     /translation="MLDKDLVRKKLNELHKHLKELEPLTQVTLSEYRVDYVRRHAAEK
                     LVELIVEYAIDINRMIVEAARADPPQTAYDTFLEIERLGVIPADLTPRLASTTGLRNR
                     LVHRYETIDNKAVYYALKPLIQHYRQYARWIRAYLTGEEAKRPRRKRGTD"
     gene            5148..6587
                     /gene="gatB"
                     /locus_tag="AC162G_07705"
     CDS             5148..6587
                     /gene="gatB"
                     /locus_tag="AC162G_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR5075734.1"
                     /translation="MNYEAVIGLEVHAELLTQSKMFCACRVVTSEAAAPNSVVCPVCS
                     GMPGVLPVINRQAVRYTIMTGLALNCTISEFSLFARKNYFYPDLPKGYQISQYEYPLC
                     KNGWLEIDLESGERKRIGIRRVHLEEDTGKTIHVDGKSLVDLNRSGVPLIEVVSEPDL
                     RSAEEVHAYAKKLRAILRYLGVNSGDMEKGVIRFEPNISIRPLGSVEFGTRTELKNLN
                     SFRALLRGTEYELARQEKVLESGGQVEQETRGWDEARGVTVTQRSKEYADDYRYFPEP
                     DLPPLIVDPAWLEEIRASLPELPDARRERLEREYGLSRKDALRLTDEKAVADYFEEAA
                     GAQRSRAQTVANWILTDLFRHMNESGQSIESVKVRPAALAGLIDLVESGAINAITGKR
                     VLSEMFESGESARAIVERAGLAQISDTGALEEAVRKVVEANPAEVEKYLSGKETVLGW
                     LVGQVMKETRGKANPNLAREALLKQLSDR"
     gene            complement(6893..7582)
                     /locus_tag="AC162G_07710"
     CDS             complement(6893..7582)
                     /locus_tag="AC162G_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIR5075735.1"
                     /translation="MTYATDLIPMVIEQSARGERAFDIYSLLLRNRIVFLGTPINDQV
                     ANVLVAQLLWLNQEDSERDIQMFINSPGGVINAGLAIYDVMQYIQAPIVTIAVGLAAS
                     MGTVLLAAGRKGKRLALPNATIHLHQPLGGVQGQATDIEIEAREILRMRDLLNRLLTR
                     HTKMNDEQILKYTDRNFYMTPELAVELGVIDEVILPHDERAAMAAALPGASTTALEAP
                     HDGDKVKAAAE"
     gene            complement(7649..8989)
                     /gene="tig"
                     /locus_tag="AC162G_07715"
     CDS             complement(7649..8989)
                     /gene="tig"
                     /locus_tag="AC162G_07715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR5075736.1"
                     /translation="MKVTTEARENRQVALTIEVEPERVEAALAKAARSIANKANIPGF
                     RKGKAPREVIERMFGKGALLEEVIDDLGQQLYREALEQESIEPYGPGRIEHIQPEPLV
                     LQMVVPLAPAIDLGDYRSLRVPFEVPEVKDSEIDHQIEHLRERHAIVEPAPEGMPADW
                     GHMVTLNIESTVEGRPFFARQDASLTLEKEHLDGVIPVVPGFEGQIVGMKAGDERTFS
                     LPVPASTDEADYGEFSGKTAEFKVSLQEIRLRTLPDADDALAQTVGDFETIGALRDAI
                     RQDIADANRREAESAYVDRVIDEALKAAQIEFPPLMVDEELDEMLERTDKRVRGQGLS
                     LEEYLKVLGKTRDEYRAEMRPTAEARIQRGLLLSRVVEREGLSVDDSEIDRGIEAVSA
                     AYGQQAGAVRETLSKSESRRRLELELLTQKALNRLATIAKGEAPPLAEPAEASA"
     gene            complement(9153..9235)
                     /locus_tag="AC162G_07720"
     tRNA            complement(9153..9235)
                     /locus_tag="AC162G_07720"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9200..9202),aa:Leu,seq:tag)
BASE COUNT         1822 a         2841 c         2972 g         1768 t
ORIGIN      
        1 gtgttcacgc tgaatgccaa cctggcgggc atctgcggca tctcgctgcc gtgcggcttc
       61 gacgcgcagg ggcttccgat cggcctgcag atcaacggcc cggccttcgg cgaagagaag
      121 atgttgcgcg tggcgtatgc ctatgagcag gcgaccgagt ggcatcggcg gagacccgcc
      181 ttatgagcga cgaactagac gttggcggtt ggatacagta tgcccaggcc gatcgacacg
      241 ctgcccaaag cctgtttgcc agcggcgact acgccgtgtg cgctgagatg tgtcaacaga
      301 tgctggaaaa gatcatcaag gcgctgatcg tctctcaaac aggcgagcgt cctccctatg
      361 aacacaattt gaaaaggctg gccgacatgg tcgttgggat tgcgattcca tcgcgcatcg
      421 ttgatattct gctcaatgcg agtccgcatt atcgcttcgc tcgctatccg ggatttggtg
      481 acttgtcgct ctatactgcg gaatacacgc ggaatctgct cgatgaagcc gaagaggcat
      541 acggatggtt cttacagcag atcaagtgag aactcagata ctgcgcttcg ttgaggctct
      601 acagcagcgc atgcgagttg aacgcgctgt gttgtttggc tcgtatgctt atggaaagcc
      661 tcacgaaggt agtgatattg acctggcaat cgtgtcgccg gactttgcag caatgaatcg
      721 cctggaacgc ctggtgtttc ttgagcgtat tgcatgggac gccgacgcgc attatatcga
      781 gccggttggc ttcacagcag aggaactgca gaacgccagc aggacgagtg tcctgagcga
      841 gattcgagat cgaggcgtgg tcatccgttt agacgaggca tccgttgaac cgctcgttgt
      901 ccgagacgat cgagcagact attcggttga ggaagacgga gacgcatgaa agtcatcatc
      961 cccctgggcg ggctgggcac gcggctgcgg ccgcatacct atacgcgccc caagccgttg
     1021 atcaacgtcg ctggcaagcc gatgctgggc cacatcgtcg acaagttcgc cggtctgccc
     1081 gtcgaggagt tcatcttcgt cgtcggatac ctgggcgagc agatcgagca ctacgtcaag
     1141 agcacctacg ccttccccgc gcgcttcgtc gagcagaaag aggcgctggg gcaggcgcac
     1201 gcgctgtggc tgtgccggga acacatcgcg gggccggtgt tcgtgatctt cggcgatacg
     1261 ttgttcgacg ccgacctgag cgagctgagc cggacgccgc aggataacct ggcttacgtc
     1321 aaagaagtcg aggatccgcg ccgcttcggc gtcgccgcgc tgcagcccga cggaggcgtc
     1381 gagcgcttcg ttgagaagcc gaatacgatg gccaaccggc tggcggtcat cggcatgtac
     1441 tactacaccg acggctcgca attgatgagc gcctgcaagg agctgatgga tcgccgcatc
     1501 caaacgaagg gggagtattt cctggccgac gccaacaatc tgatgatcgg gcagggcgcg
     1561 cgcttcgtca cccgcaccgt cgacgtgtgg ctggacaccg gcaagccgga gacggtcctg
     1621 gagacgaatc gctatttgct cgacaacggt agcgacaaca gcggccaggt gcactttccg
     1681 gcctgcgtca tcgtgccgcc ggtcaacgtc catcccacgg caaagatcga agaagcggtc
     1741 atcgggccgt acgcgacgat cggcgagaac tgccgtatcc gccgggcgat tgttcgcgac
     1801 tcgatcatcg aggccgacgc gacgatcacc gacacgttcc ttgaccagtc gctcatcggc
     1861 cgcgaggcga tggtcacggg acggtatcgc aagttcaacg tcggcgattc ggccagcgtg
     1921 ggattcagct agacggcggt ctccgcgtaa cgtgaattat ggatacgata tccgtgcacg
     1981 tcgaatgcta ctccagccac aggtacgccc aggaacctcg cgccatcgtc tggcgcggat
     2041 tccgtgtgcc gatcgcggcc atcgagaaca tgtggcgcac gccggacggg ccgctgttcc
     2101 gcgtccggct cgatgacggc gccatcgtcg atctgcaata cgtcgaggcg agcgacgagt
     2161 ggctcctgac cgatcgcctc ttgcctcgcc gttaggacgg gaactatgcg caataccatt
     2221 tcgcaacgtg tcgcatccgt gcctcccagc ggcattcgcc gcttcttcga catcgccgcg
     2281 acgatgaaag acgtgatctc gctcggcatc ggcgagccgg acttcacgac gccgcagaat
     2341 atcctcgacg ccggcgtcgc ctcgctgcac aagggcgaga cgcattacac cagcaactcc
     2401 ggcacgattg agctgcgccg cgcgctgagc gaccatctgc gccggctgta cggcgtgcgt
     2461 tacgatcccg agtcggaatt gctgatcacg gtcggcgtat ccgaggcgct gtacctggcg
     2521 ctgacggcga tcctcgatcc gggcgacgag gtcatcgtcc cgcagccgtg cttcgtcgcc
     2581 tacacggccg aggtcgtctt cgccggcggc gtgcccgtgc cgatcgccac gaaggtcgag
     2641 aacaacttcc aggtgaccgg cgcggagatc gcggcgaaga tcacgccgaa gacgaaggcg
     2701 atcctgatcg gctaccctaa caatccgacc ggcgcggtga tgagccgcga gcggctgatg
     2761 gagatcgcgc agctcgccga gcagcatgac ctgctggtgg tctcggacga gatttacgac
     2821 aggctggtgt acgcgccgat ggacggcgat gggatggggg cgcagcatac ctgcttcccc
     2881 tcgctgccgg gcatgcagga gcgcacgctc ctgctcggcg gcttctcgaa ggcctacgcg
     2941 atgaccggct ggcgcatcgg gtacgccgcc gcacccgccg agctttcggc ggcgatgcgc
     3001 aaggtgcacc agtacaccat catgtccgcg ccgacgacgg cgcagaccgc cgcactcgag
     3061 gcgctgcagc gcggcgagga gcatgtcgag gcgatgcggc aggagtacga ccggcgacgg
     3121 cggctgatcg tgaggggcct gaacgagatc gggctgaaga cgttcgagcc acagggcgca
     3181 ttctacgcct tcccgtcggt cggcgtgtcc ggcatgagcg acgaggagtt cgtcgagcgg
     3241 ctgctgaacg aggagcaggt cgcggtcgtg ccgggcaacg ccttcggcgc gggcggcgca
     3301 ggcttcgtgc gctgctcgta cgccacggct tacgagaaga tcgagcaggc actggagcga
     3361 atgcatcgct tcttgcggcg gcatgggtga ggcaacggat atgcaacgga taaacggatg
     3421 cgggttggag gaatgggaaa acgcggtgaa gtattggtcg caatcatgaa cgatctccgc
     3481 gacatggaga tcgcccgcag ccagcactgg tatcgcattc ctgtgcgcag tgtggcaaag
     3541 cttctgaagg aacgctggcc tccacaatgg ctggcctttt accaaaccaa agaattcggt
     3601 gataaggcac atgcagtgaa ctacttctcg caggtaatcg acatccgcga ggtcttccgc
     3661 tggcagctct ttccgaatca accaatggat gagaagggcc gacaacgcta ctaccagttg
     3721 atattgtcac ccttgcagcg gcttccccaa ccgattccca gccgccggtg gcggctcatc
     3781 gttttcattc ccaccacttg ggaaaagctc atgaatgcag tcgagatcaa tgacttgtat
     3841 gatgaaagcc cattggaaga ccgtctatgg gctgaattca agcgactgaa aattgctgca
     3901 gagcgacaag agtatgtcca agtcggtgac catcgttatg ccctcgactt cgctatttat
     3961 tgtgctacag gcaaaataga tgtcgaaacg gacggcgata cctggcatgc cagttcggag
     4021 aagagccaac tggacaacat tcgtgacaac gatctcaaga cgcttggctg gcgaacattc
     4081 cacttcacca ctcaccagat tcaggagcaa gctgcggaat attgtatttc cacgattgtc
     4141 gaaaatatca atcgattggg tggccttgag gaaggacggt tagcgccgcg caagatcaac
     4201 ctggacggat tggaacaatt gactctacct gatcccgaag tgcacgacga tcctgactga
     4261 gagcgaagag tgaagtgaag ggagtttcac cagcgatgag aattccttcg aaggcgcaac
     4321 tcgaacctct tgccaggcgc tatggcctgc ggctgatcgt gctgttcggc tcgcaggtga
     4381 ccggccacac gcacccggag agcgatgtgg atgtggcggt gtgggcggct cgcccgatta
     4441 ctcccggacg gcgcgatcga ctgtggcgcg agctaaccga tctgtttcag actgagatcg
     4501 atctggcctt gctgaaccac gctgagcctc tgctgatgaa acacgtggct cgcaccggcc
     4561 ggctcttata cgaaggccgg cgctgggcgt gggaagagtt caagggattt gcattccggt
     4621 attacctcga cacccgcaaa ttcagagacg accttgcgcg tcacctcgac cgcgagattc
     4681 gccggccgcg ccatgctgga taaagacctc gttcggaaga agctgaacga gctgcacaaa
     4741 cacctgaaag aactggagcc gctcacacag gtgacgctgt cggagtacag ggtcgactac
     4801 gtgcggcgac atgccgccga gaaactggtc gagctgatcg tcgagtatgc gatcgatatc
     4861 aatcgcatga ttgtcgaggc cgctagagcc gatccacctc agactgctta cgacaccttt
     4921 ctcgaaatcg agcgacttgg cgttatccct gccgacctga ctccccggtt agcctcaact
     4981 accgggctgc gcaatcgttt agttcatcgc tacgagacga tcgataacaa ggccgtgtat
     5041 tatgcgctga aaccgctgat acagcactac cgccagtatg cgagatggat acgggcctac
     5101 ctgactggtg aagaagccaa gcgacctcga aggaaacggg ggaccgattg aattacgaag
     5161 ccgtcatcgg actcgaagtc cacgcagaac tattgaccca atcgaagatg ttctgcgcct
     5221 gccgggtggt cacctcggaa gccgccgcgc cgaactcggt cgtgtgcccg gtgtgctccg
     5281 gcatgcccgg cgtgctgccc gtgatcaatc gccaggcggt gcgctacacg atcatgaccg
     5341 gcctggcact gaactgcacg atcagcgagt tcagcctgtt cgcgcgcaag aactacttct
     5401 accccgacct gccgaagggg tatcaaatct cgcagtacga ataccccttg tgcaagaacg
     5461 gctggctgga gatcgacctc gagtcagggg agcgcaagcg catcggcatc cggcgcgtgc
     5521 acctggaaga ggataccggc aagacgatcc acgtcgacgg caaatcgctc gtcgatctga
     5581 accgcagcgg cgtgccgctg atcgaggtcg tgtcggagcc tgacctgcgc tcggcggaag
     5641 aagtgcacgc gtacgcgaag aagctgcgcg ccatcctgcg ctatctcggc gtgaactccg
     5701 gcgacatgga aaagggcgtc attcgcttcg agccgaacat ctccatccgg cccctcggct
     5761 cggtcgaatt cggcacgcgc accgagctga agaacctcaa ctcgttccgt gcgctgctgc
     5821 gcgggaccga gtacgagctg gcgcgccagg agaaagtgct cgaaagcggc ggtcaggtcg
     5881 agcaggagac gcgcggctgg gacgaggcgc gcggcgtcac cgtgacgcag cgcagcaagg
     5941 agtacgccga cgactatcgc tactttcccg agccggacct gccaccgctg atcgtcgatc
     6001 cggcgtggct cgaagagatt cgcgcctcgc tgccggagct ccccgacgcg aggcgcgagc
     6061 gcctcgagcg cgagtacggc ctgagccgca aggacgcgct gcgactgacc gacgagaagg
     6121 cggtcgccga ttatttcgaa gaggcggccg gcgcgcagag gtcgcgggcg cagaccgtcg
     6181 ccaactggat tctgaccgat ctgttccggc acatgaacga gtcgggccag agcatcgagt
     6241 cggtcaaggt gcgaccggcg gcgctggccg ggctgatcga cctcgtcgag tcgggcgcga
     6301 tcaacgccat caccggtaag cgtgtgctga gcgagatgtt cgaatccggc gaaagcgcgc
     6361 gcgcgatcgt cgagcgggcg gggctggcgc agatcagtga cacgggcgcg ctggaggagg
     6421 ctgtgcgcaa ggtcgtcgaa gccaacccgg ccgaagtcga gaagtatctc tctggcaagg
     6481 agacggtgct gggctggctg gtcggccagg tgatgaaaga gacgcgcggc aaggcaaacc
     6541 ccaacctcgc gcgcgaagcc ctgctcaagc agttgagcga tcgctgaagc ggatccgcca
     6601 cggttggagt gcaggcttca gcctgcgaag cgccccagat atggcagcct gaccccactc
     6661 cgcttcgcta cgggggcgct tcgcagaggc tgcactccag gaattcgcca gactctagca
     6721 agaaacacaa gaacctgcgg atgggtgagc agtgcataag tcgaggccac ctttcacagg
     6781 tggtctcgct cttttcatcg actaaggtac agacttccga agtctccaag acttcggaag
     6841 tctggcgaac gcaaatcgaa ttggaactga ctctataggt aaagtagaag gtctactcgg
     6901 cggctgcttt gaccttgtcg ccgtcgtgcg gcgcttcgag agccgtggtc gacgccccgg
     6961 gcagcgcggc ggccatggct gcgcgctcgt cgtgtgggag gatcacttcg tcaatcacgc
     7021 ccagctcgac ggccagctcg ggggtcatgt agaagttgcg atccgtgtac ttgagaatct
     7081 gctcgtcgtt catcttggtg tggcgggtga ggagacgatt gagcaggtcg cgcatgcgca
     7141 ggatctcgcg cgcctcgatc tcgatgtcgg tggcctggcc ctgcacgccg cccagcggct
     7201 ggtgcaggtg gatggtggcg ttcggcagcg ccaggcgctt gcctttcctg ccggccgcca
     7261 gcaggaccgt gcccatgctg gccgccaggc caacggcgat cgtcactatc ggcgcctgga
     7321 tgtactgcat cacgtcgtag atcgccaggc ccgcgttgat cacgccgccg gggctgttga
     7381 tgaacatctg aatgtcgcgc tcggagtctt cctggttcag ccacagcaac tgggcgacga
     7441 ggacgttggc cacctgatcg ttgatcggcg tgccgaggaa gacgatgcgg ttgcgcagca
     7501 gcagcgagta gatgtcgaag gcgcgctcgc cgcgcgcgct ctgctcgatg accatcggga
     7561 tcaaatcggt tgcgtaagtc attctgtatg cctctcctat ggatgcaatc cgcgcgaggg
     7621 tttcaatcct tcgcgagggt cttgagattc aggccgatgc ttcggccggc tcggccagcg
     7681 gaggcgcttc gcctttggcg atggtcgcca gtcgattgag cgccttctgg gtgagcagct
     7741 cgagctccag gcgacgccgg ctctcgctct tcgacaaagt ttcgcgcacc gcgccggcct
     7801 gctggccgta ggcggcgctg accgcctcga tcccccggtc gatttcggag tcgtccacgc
     7861 tcagcccttc cctctccaca acgcgactca gcagcaagcc gcgctggatg cgggcttcgg
     7921 cggtcgggcg catttctgcg cggtactcgt cgcgcgtctt gcccagcact ttgagatact
     7981 cctccaggct gaggccctgg ccgcgcacgc gcttgtcggt gcgctccagc atctcgtcaa
     8041 gctcctcgtc gaccatcagc ggcgggaact cgatctgggc agccttgagc gcctcgtcga
     8101 tcacccgatc gacgtaagcc gactcggctt cgcgcctgtt cgcgtcggcg atatcttggc
     8161 ggatcgcatc gcgcagcgcg ccgattgtct cgaaatcgcc caccgtctgg gccagcgcgt
     8221 cgtcggcatc gggcagcgtg cgcagccgaa tctcttgcag ggacactttg aactcggcgg
     8281 tcttgcctga aaattcgccg tagtcggcct cgtccgtgct cgcgggaacc ggcaggctga
     8341 atgttcgctc gtcgccggcc ttcatgccga cgatctgccc ctcgaagccg ggcacgaccg
     8401 ggatgacgcc gtccaggtgc tctttctcga gggtcaggct ggcatcctgc cgcgcgaaga
     8461 aaggccggcc ttcgacggtc gattcgatgt tcagcgtgac catgtggccc cagtcggcgg
     8521 gcatcccttc gggagccggc tcgacgatcg cgtgacgctc gcgcaggtgc tcgatctgat
     8581 gatcgatttc actgtccttg acctccggta cctcgaacgg cacgcgcagg ctgcgatagt
     8641 cgcccaggtc gatggccggc gcgaggggga cgaccatctg caggacgagc ggctccggct
     8701 ggatatgctc gatccggcca gggccgtaag gctcgatgct ctcctgctcg agcgcttcgc
     8761 ggtaaagctg ttggccgaga tcgtcgatca cttcttcgag cagcgcgcct ttgccgaaca
     8821 tgcgctcgat cacctcgcgc ggggccttgc ccttgcgaaa gccggggatg ttggctttgt
     8881 tggcgatgct ccgcgcggct ttcgccagcg ccgcctcgac gcgctccggc tcgacctcga
     8941 tcgtcagcgc gacctggcga ttctcgcgcg cttcggtagt aactttcacg gatgtcccta
     9001 ctttcggaaa tcggtctgaa acattatatc acgtttcacc cgcgcctgac gcagaatcgc
     9061 atatcgccta tcgcttatcg cgtatcgtgc ggcgcttggc tgcacgagca tcgacacacg
     9121 ataagctatc agcgatatgc gattcgccga aatgccggag gcgggacttg aacccgcacg
     9181 ctgttgccag cacaggttcc taagacctgc gtgtctgcca ttccaccacc ccggcgggcg
     9241 cattcgatta taccgtgttc gcactgcttg gcaaggctat gccacgccgt cgcgcgttcc
     9301 acgccgcagg caggccgggg gaggtgtagt taaggttcac tgcgcgcggc aggcgcagtt
     9361 gcatcttgac aacgcaattc agtggaccgc actagagcgg att
//
LOCUS       DBNHXB010000191         8099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3566441, whole genome shotgun sequence.
ACCESSION   DBNHXB010000191 DBNHXB010000000
VERSION     DBNHXB010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3566441"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3317
                     /locus_tag="AC162G_07725"
     CDS             <1..3317
                     /locus_tag="AC162G_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5075737.1"
                     /translation="LQKVVPYDSSSVLQVKGDRLVIIGGRGFPNPDEILGLSFGLTDE
                     SSPSTEVARRRAPYILEDAPNLYRGFRAEPHAEAGIRSWMGVPLLFGDRLIGMFTLDK
                     RQPGFYTEAHARLALAFAAQAAVAIENARLFEETQQGIAELAIINSIGQALASQLELN
                     ALIELVGEKLRLIFDVQYVFIALLDRQTPADSARGQAGIISFPYYWDLDHREASEETF
                     RLGEGLTSRIIESRQPQLINFDWERRAIELGAIFTGEPVKCSLGVPILVGDEAIGAIM
                     LQSTQREGVFTDSDVRLLTTIAANVGVAIQNARLFQETTRLLEQSQQRAAELAVINSV
                     QQALASQLDFQAAIEMEGDKIREVFKAQGLYIALVDQASGTIHFPYWLEQGQRFQVPA
                     RPLGAGFTSHVIRTRRPFVINRDIEKRRAELGASSSQSGPPIKAYAGVPLLAGEDVIG
                     VIALGNWDHEDAFSDSDVRLLTTLASSMSVALQNARLFGETKRLLEQSRQRAAELATI
                     NSIVQALASQPELHALIDLVGEQMRQIFDAQIVYIALLDRRTDLIHFPYYHEIDRRIA
                     ARPLRLGEGWASKIIQSRQPMLINKDLAQRRAALGIEIIGSPSKTYLGVPITVGDESI
                     GVISVQNTEREEVFSEADVRLLTTIAANVGAAIERARLYASAQEQKQYFESIVLYNPV
                     AIVTMDPNATVLSWNPAAERLFGYTASEAIGCNIDDLIATSQAIHAEAIAYSRQAMSG
                     ERLHVITRRNRKDGTLVDVEVLAVPVEVGGQPQAYVAIYHDITDLQRARREAETANQA
                     KSAFLANVSHELRTPLTSVLGFAKIIKKRLDEVIFPKVRDDDSRTRRAVAQVRDNIDI
                     IVSEGERLTALINDVLDLAKIEAGRVEWQAQPVRIADVIERAATATASLFAAKRLAQI
                     TDIDPDLPEIVGDHDRLVQVMINLISNAVKFTERGSVTCRARLSPALAPSARESQRSG
                     GGRSPTTPLGGAQAAAGGPGGQDIVVSVIDTGVGIARDDLSKVFEQFVQVGDTLTDKP
                     QGTGLGLAISKQIVEHHGGRIWVESVVGAGSTFSFTLPVDGAASGDSQSSVISNRYSE
                     RLAETVPSDRTVRSNE"
     gene            3310..3735
                     /locus_tag="AC162G_07730"
     CDS             3310..3735
                     /locus_tag="AC162G_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5075738.1"
                     /translation="MSERFIRQFPAKLSMLVEIRRYVEDTAAGFGANDSALLDVVQAV
                     DESAANIIMHGYRGGPGTIDVEIRRERDSLTVCLRDRAPLFDPTRVPAPDLRLPLDQR
                     PLGGMGIYLTRKFVDELAYRVTPEGGNELTLRKSLSSGQ"
     gene            3911..4318
                     /locus_tag="AC162G_07735"
     CDS             3911..4318
                     /locus_tag="AC162G_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR5075739.1"
                     /translation="MNITVENAQGRVPVTILELHGDLDGSNYQEVIARARDLYGAGMR
                     HLLIDMGDMAYMSSAGLVALHSVILLLRGEQPPDPESGWSAFHAIAREQGGVQKAVKL
                     LNPQPRVRRTLEMTGMQEFFEIYADRLAAVESF"
     gene            4385..6205
                     /locus_tag="AC162G_07740"
     CDS             4385..6205
                     /locus_tag="AC162G_07740"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR5075740.1"
                     /translation="MVPLHRLIERENAETLVREFAALLPGVDFALVDPDGRVLAGAGE
                     WPRSPASAEALTLTLNKVKGKGKEQQRGTPAMALALVKDERIVHADGLTILPLWVDAA
                     LVGALIAKESSPLLSSATGEGPGVRALRCLYACASMLIAQTIETRDIARETLERYREI
                     NLLYQIGETIGSCLDAAEIPGMALAEAQRVIRAEAGAVLLPASDDGDAWEVKAGFGED
                     ADFMLLSDLARPLIERARVKGIPDIVERAPGDSPLPRPFDPSAPLRAGFAQGRLRSEP
                     APSPARGQGFSSLLCAPLKTRERTLGALLLGRSAGRAAFTASDEKLAMAIAGQAAIAI
                     EKAWLHRQEIERQRMEEELSVGRRIQLSLLPQACPDSPGWAFAASYRAAREVSGDFYD
                     FFEAPDDPARLRIVIGDVTGKGVPAALMMAFSRALIRAESTSGRHPAEVLERVNRAII
                     WNRSRLLLTAFYAALDRRSGRMTFASGGHDWPLWLKAATGECIELETRGVVLGALNEI
                     PLVDRVIDLAPGDLVVCFTDGVTEAQNAEGQMFDLERLKAVVKANRHAGAQRALTAIV
                     EAVEGFVGDTPQSDDLTVVVVKRTSRVEEASHGAESADRG"
     gene            6177..6554
                     /locus_tag="AC162G_07745"
     CDS             6177..6554
                     /locus_tag="AC162G_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006101731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5075741.1"
                     /translation="MGQKVLIVDDEAHIRLLIQQTLEELEERGVEILTAANGEQALEI
                     IRADKPDLIFLDVMLPKMNGFDVCDRVRNDWGMRDVYIVMLTAKGQEFDRQKGERVGA
                     NLYMTKPFDPDELLRKAVEVLGL"
     gene            6619..6984
                     /locus_tag="AC162G_07750"
     CDS             6619..6984
                     /locus_tag="AC162G_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR5075742.1"
                     /translation="MNASSTLRADCIFCKIVANQASGHVLYQDDQVTAFRDIYPVAPT
                     HILIVPNKHLESTRDLTPEDAPLAGHMLIVAHRLAEQAGIQDSGYRLIVNTGPDANQR
                     VFHLHLHLIGGQRMRYPMG"
     gene            complement(7006..7161)
                     /locus_tag="AC162G_07755"
     CDS             complement(7006..7161)
                     /locus_tag="AC162G_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR5075743.1"
                     /translation="MGNGAITTHTYAAAGAYTAIVTATNGTSLVTATTQVLIVTSRIY
                     LPLVMRN"
     gene            7475..7939
                     /locus_tag="AC162G_07760"
     CDS             7475..7939
                     /locus_tag="AC162G_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075744.1"
                     /translation="MEGTDNGGYSWTLGIKLTNPDEGARNFIYVSAIPKGFQSEGDEG
                     IYNYDPAEAEILLNLRVGESKSLREGLDTAQWFTYTRQPDTIISGQAAKTYENIQPWE
                     FPEGTKEIRYYLQADEYTYLIGGYLDTTDSNQPGAITEELFDQIIATFRLNP"
BASE COUNT         1551 a         2474 c         2672 g         1402 t
ORIGIN      
        1 agctgcagaa agtggttccg tacgacagct cgtcggtgct gcaggtcaag ggcgatcgat
       61 tggtcattat cggcggccgc ggcttcccga atccggacga gatcctgggc ctcagcttcg
      121 gcctgacgga tgagagcagc cccagcacgg aagtggcgcg ccggcgcgcg ccgtatatcc
      181 tggaagatgc gccaaacctg tacaggggct tccgcgccga gccgcacgcc gaggccggca
      241 tccgctcgtg gatgggcgtg cccctgctgt ttggcgatcg cctgatcggc atgttcacac
      301 tggacaagcg ccagcccggc ttctacaccg aggcgcacgc ccgcctggcg ctggccttcg
      361 ccgcgcaggc ggccgtcgcc atcgagaacg cgcggctgtt cgaggagacc cagcagggca
      421 tcgccgagct ggcgatcatc aacagcattg gacaggcgtt ggcctctcaa ctggagctca
      481 atgcgctgat cgaactggtc ggcgagaagc tgaggctgat cttcgacgtg caatacgtct
      541 tcatcgcgct gctcgaccgg cagacccccg cagacagcgc gcgagggcag gcgggcatca
      601 tcagttttcc ctactactgg gatctggatc accgcgaggc ctccgaagag acgttccggc
      661 tcggtgaagg actgacctcg cgcatcatcg aatcccgcca gccgcagttg atcaacttcg
      721 actgggaacg gcgggcgatc gagctgggcg cgatcttcac gggcgagccg gtgaagtgct
      781 cgctgggcgt gcccatcctg gtcggcgacg aggccatcgg tgcgatcatg ctgcagagca
      841 ctcagcgcga gggcgtattc actgactcgg acgtgcgcct gctgacgacg atcgcggcca
      901 acgtcggcgt ggctatccag aacgcgcggc tgtttcagga aaccacccgc ctgctggagc
      961 aaagccaaca gcgcgctgcc gagctggcgg tcatcaacag cgtgcagcag gcgctggcct
     1021 ctcaactgga cttccaggct gcgattgaga tggagggcga caagattcgc gaggtgttca
     1081 aagcgcaggg cctgtacatc gcgctggtcg atcaggctag cggcacgatt cacttcccgt
     1141 actggctgga gcaggggcag cgctttcagg tccctgcgcg tcccctcggc gcgggcttta
     1201 cctcgcacgt cattcgaacg cgccggccgt tcgtcatcaa tcgtgacatt gagaagcgca
     1261 gggccgaact gggcgcatcc agctcgcaaa gcggcccgcc gatcaaggca tacgccggcg
     1321 tgccgcttct ggccggcgag gatgtgatcg gcgtcatcgc gctgggcaac tgggatcacg
     1381 aggatgcctt cagcgattcc gatgtgcgtt tgctgacgac gctggcgtcg agcatgagcg
     1441 tggcgcttca aaatgcgcgg ctctttggcg agacgaagcg cctgctggag cagagccggc
     1501 agcgcgccgc cgagctggcg acgatcaaca gcatcgtcca ggcgctggcg tctcaaccgg
     1561 agctccacgc gttgatcgat ctggtcggcg agcagatgcg ccagatattc gacgcacaga
     1621 tcgtgtacat cgcgctgctc gaccggcgga ccgatctgat ccacttccct tattatcacg
     1681 agatcgaccg gcgcatcgcc gcccgaccct tgcgactcgg cgaaggctgg gcgtccaaga
     1741 tcatccaatc gcgccagccc atgttgatca ataaggacct ggcccagcgg cgcgcggcgc
     1801 tgggtatcga gattatcggc tcgccgtcga aaacttatct gggcgtgccg atcacggttg
     1861 gcgatgagtc tatcggcgtg atcagcgtgc aaaacacgga acgggaggag gtcttcagcg
     1921 aagccgacgt gcgcttgctg acgacgatcg cggccaacgt cggcgcggcg atcgagcgcg
     1981 cccggctgta cgcctcggcg caggagcaga agcaatactt cgagtcgatc gtgttgtata
     2041 atccggtggc gatcgtgacg atggatccga atgcaacggt tctctcgtgg aacccggcgg
     2101 cggaacggtt gttcggctac acggcgagcg aggcgatcgg ttgcaacatc gacgacctga
     2161 tcgcgacgag ccaggctatc catgcggagg cgatcgccta ttcccgacag gccatgtccg
     2221 gtgagcgctt gcacgtcatt acgcggcgca accgcaagga tgggacgctc gtagacgtcg
     2281 aagtgctggc cgtgccggtc gaggtcggcg gtcagccgca ggcatacgtc gccatttatc
     2341 acgacatcac cgacctgcag cgcgcccgcc gtgaagccga aacggcaaac caggccaaga
     2401 gcgctttcct ggccaacgtc agtcacgagc tgcgcacgcc gctgacgtcc gtcctgggct
     2461 tcgccaaaat catcaagaag cgtctggacg aggtcatctt ccccaaggtt cgggatgacg
     2521 acagccggac gcggcgcgcc gtcgctcagg tgcgcgacaa catcgacatc atcgtcagcg
     2581 agggcgagcg gctgacggcg ttgatcaacg acgtgctcga cctggccaag atcgaagccg
     2641 ggcgggtcga atggcaggcg cagcccgttc ggatcgccga cgtgatcgag cgcgccgcga
     2701 cggccacggc gtccctgttc gccgcgaagc gcctggcgca gatcacggac atcgatccgg
     2761 acttgccgga gatcgtcggc gatcacgacc gcctcgtcca ggtcatgatc aatctcatct
     2821 cgaacgcggt gaagttcacc gagcgcggct cggtgacgtg ccgcgcccgc ctgtcacctg
     2881 ccctcgcgcc ttcggcgcgg gagtcgcagc gtagcggagg gggtcgcagc cccacaactc
     2941 cgctgggcgg ggcgcaggca gccgccggcg ggccgggcgg gcaggacatt gtggtgagcg
     3001 tgatcgacac cggcgtcggc atcgcgcgcg acgacctctc caaggtcttc gagcagttcg
     3061 tgcaggtcgg tgacacactc accgataagc cgcagggcac ggggctgggc ctcgcgatca
     3121 gcaagcagat cgtcgagcac cacggcggcc gcatctgggt ggagagcgtc gtgggcgcgg
     3181 gcagtacgtt ctccttcacg ctgcccgtcg acggggcggc gagcggcgat agtcagtcat
     3241 cagtcatcag taatcggtat tcagagcgcc tggcggagac ggtgccatcg gatcgaacgg
     3301 tgcgatcgaa tgagtgagag attcattcgg cagtttccgg cgaaactgag catgctggtg
     3361 gagatccggc gctacgtcga agacaccgca gccggtttcg gcgccaacga ctccgccttg
     3421 ttagatgtgg ttcaggcggt ggacgaatcg gccgccaata tcatcatgca cggctaccgt
     3481 ggcgggccgg gcacgatcga cgtcgaaatc aggcgcgagc gcgattcact caccgtttgc
     3541 ctgcgcgatc gggcgccgtt gttcgatccc acccgcgtgc cggccccgga cctgaggctc
     3601 ccgctggacc agcggcctct cggcgggatg gggatttatc tgacccggaa gttcgtggac
     3661 gagttggctt atcgcgtcac gcccgagggc ggcaacgagc tgacgcttcg caaaagcctg
     3721 tcctcaggtc agtgaacagt aaccagtgac cagtgatcag taatcagtaa tcagtggtca
     3781 gtcatcagta ctggacaact gatgactgac cactgacaac tgaaaactga taactgatga
     3841 ctgacaacga agggcctcag cgagcagtga gcaagtactc aaggaaccta tctatggaag
     3901 gaagacaaaa atgaatatca ccgttgagaa cgcccagggc agggtgccgg tgaccattct
     3961 ggagctgcac ggcgacctgg acggctcgaa ttatcaggag gtcatcgcca gggcgcgcga
     4021 cctgtacggg gcggggatgc gccatctgtt gatcgacatg ggcgacatgg cgtatatgag
     4081 cagcgccggc ctggtggcgc tgcacagcgt gatcttgctg ctgcgcggcg aacagccgcc
     4141 cgatccggag tcgggatgga gcgcgtttca cgcgatcgcc cgcgagcagg gcggcgtgca
     4201 gaaggccgtc aagctgctca acccgcagcc gagagtgcgc cgcacactgg aaatgacggg
     4261 catgcaggag ttcttcgaga tctacgccga tcggctggcg gccgtcgagt ccttctgacg
     4321 caggtttggt cggtgggtcg ggtggtcacg tagtcgaatg gtcgggtagt cgggtggtca
     4381 aggtatggtt ccgctgcatc gcttgattga gcgcgaaaac gccgagacgc tcgtgcgcga
     4441 gttcgcggcg ctcttgcccg gcgtcgattt cgcgctggtc gacccggatg ggcgtgtgct
     4501 ggccggcgcg ggcgaatggc ctcgatcgcc tgcctcagcc gaagccctga ccctgaccct
     4561 gaacaaagtg aaggggaagg ggaaggagca gcagcggggg acaccggcga tggcgctggc
     4621 tctcgtcaag gacgagcgca tcgtccacgc cgacgggctg accatcctgc cactctgggt
     4681 cgacgcggcg ctggtcggcg cgctgatcgc gaaggagtcc tcccctctcc tgtcgtctgc
     4741 gacgggagag gggccggggg tgagggcgct gcgctgtctg tatgcttgcg cgtcgatgct
     4801 catcgcgcag acgatcgaaa cgcgcgacat cgcccgcgag acgctggaac gctaccggga
     4861 gattaacctc ctgtatcaga tcggcgagac gatcggcagt tgcctggacg cggctgagat
     4921 tccgggcatg gcgctggccg aagcgcagcg cgtcattcgg gccgaggcgg gcgccgtcct
     4981 gctgccggct tcagacgacg gagatgcctg ggaggtgaaa gccggcttcg gcgaagacgc
     5041 cgacttcatg ctgctgtctg acctggcgcg gcctttgatc gagcgcgcgc gggtcaaggg
     5101 tatccccgat atcgtcgagc gcgcgccggg cgacagcccg ctccctcgac ccttcgaccc
     5161 ttcggctccg ctcagggcag gcttcgctca gggcaggctt cgctcagagc ctgccccgag
     5221 ccctgcgagg ggacaaggtt tcagctctct gttatgcgcg ccgctcaaaa cgcgcgagcg
     5281 cacgctgggc gcgctgctgc tcggtcgatc ggccggccgt gcggcattca cggcgagcga
     5341 cgagaagctg gcgatggcga tcgccggcca ggcggccatc gcgatcgaga aggcgtggct
     5401 gcatcgccag gaaatcgagc ggcagcgcat ggaggaagag ctgtcggtcg ggcggcgcat
     5461 ccagctcagc ctgctgccgc aagcctgccc cgactcgccg ggctgggcgt tcgcggcgtc
     5521 gtatcgcgcc gcgcgcgaag tgagcggcga cttttacgac ttcttcgaag cgccggatga
     5581 tccggcccga ttgagaatcg tgatcggcga cgtgacaggc aagggcgtgc cggcggcgct
     5641 catgatggcg ttcagccgcg cactcattcg cgccgaatcg acgagcggcc ggcatcccgc
     5701 cgaggtgctg gagcgcgtca accgcgccat catctggaac cgctcgcgac tgctgctgac
     5761 ggccttctac gctgcgctcg ataggcgcag cggtcgaatg acattcgcca gcggcgggca
     5821 cgattggccg ctctggctga aagcggcgac gggcgaatgt attgagctgg agacgcgcgg
     5881 cgtcgttctc ggcgcgctca acgagatccc gctggtcgat cgtgtgatcg atctggctcc
     5941 cggcgacctc gtggtatgct tcacggacgg cgtgaccgaa gcgcagaatg ccgaaggcca
     6001 gatgtttgac ctggaaaggc tgaaggcggt ggtgaaagcc aaccgtcacg ccggcgcaca
     6061 gcgtgccctg acggccatcg tcgaggcggt tgagggcttc gtcggcgaca cgccgcagtc
     6121 ggacgatctc acggtcgtcg tggtcaagcg aaccagtcgt gtcgaggagg cttcacatgg
     6181 ggcagaaagt gctgatcgtg gatgacgagg cacacatccg gctgttgatc caacagacgc
     6241 ttgaggaact cgaggagcga ggcgtcgaga ttttgaccgc cgcgaacggc gaacaggcgc
     6301 tggagatcat ccgggccgat aagcctgacc tgattttcct cgacgtgatg ctgcctaaga
     6361 tgaacggctt cgacgtgtgc gatcgggtga gaaacgattg ggggatgcgt gacgtgtaca
     6421 tcgtcatgct cacggccaag ggacaggagt tcgaccggca gaagggcgag cgggtcggcg
     6481 cgaacctgta catgaccaag ccgttcgatc cggacgagct gctgcggaag gccgtcgagg
     6541 tgctggggct gtagcgagca agatccaaac tacaaaagcc aaactgcaaa agccaaactt
     6601 caagaaccgg agatccccgt gaacgcgtcg agtactctgc gggccgactg tatcttctgc
     6661 aaaatcgtcg ccaaccaggc gtccggccac gtactctatc aagacgatca ggtcacggcc
     6721 ttccgcgaca tctaccccgt cgcgccgacc cacatcctca tcgtgccgaa caagcacctg
     6781 gagtccaccc gcgacttaac tcccgaggac gcgccgctgg cgggtcacat gcttatcgtc
     6841 gcccaccgtt tggccgagca ggccggcatc caggattccg gctatcgcct gatcgtcaac
     6901 accggccccg atgccaacca gcgggtcttc cacctgcatc tgcatctgat cggtgggcag
     6961 aggatgcgct atccgatggg atgacggcga ctcagacaca tcgcctcagt tgcgcatcac
     7021 cagcggcaga tagattcttg aagtgacgat caagacctgg gtggtcgccg tcacgaggct
     7081 cgtaccgttc gtcgccgtga cgatcgccgt ataggcgccg gctgcggcgt aagtatgcgt
     7141 tgtgattgcc ccattaccca actgaagttc cccggaaaaa gtggacaggc agataagcca
     7201 tagtacacta tccgaaagga ggaagcacat gcgcgcaaga acacaccagg ggtttttggg
     7261 ggttggtcaa cttagcccca ccctaatcct tgtaggtttg ttgttggtcg gaagcgtaag
     7321 ttgcacaccg gcaagcaccc agccggtggg tcaggctacg cccacgtcgt cggctgctcc
     7381 gtcagccacg gccacaagtg aagcgcccct gccctccggg tgggagacgc ataccagcca
     7441 gcgcttcgcg tatactatca gttatccact cgacatggaa ggcacagaca acggaggcta
     7501 cagctggacg ctggggatta aactgacgaa tcctgacgaa ggggcccgga acttcatcta
     7561 cgtgtctgcg attcccaaag gcttccagag cgagggcgat gagggcatct acaactatga
     7621 cccggcggag gcggaaatcc tgctgaacct gcgggtcggc gagagcaaat cgttgcgcga
     7681 gggcctggac acggcgcagt ggtttaccta cacgcgccaa ccggacacga ttattagcgg
     7741 ccaggccgcc aagacgtacg agaacatcca gccctgggag ttccccgaag gaaccaagga
     7801 gatccggtat tatttgcaag cggatgaata cacctacctg attggtggct acctagatac
     7861 cacagactcg aatcaaccgg gtgccatcac tgaagaactg ttcgaccaga tcatcgccac
     7921 cttccgattg aatccgtgat ggttgcctag tcctgtgtta cggctttccc tcattggggg
     7981 attgccgcag gtcgaaaggt cgcccaatcc ctgccggccg ccgaccgcac cgcgttccaa
     8041 gtagactcgt gccccgatgg gggctcttgg cgtgccatat attcgcgatc tgtgtggcc
//
LOCUS       DBNHXB010000192        15273 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4572770, whole genome shotgun sequence.
ACCESSION   DBNHXB010000192 DBNHXB010000000
VERSION     DBNHXB010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 15273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15273
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4572770"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="AC162G_07765"
     CDS             <1..310
                     /locus_tag="AC162G_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5075745.1"
                     /translation="EREAFGEPIAQRQSIAFMLAEMATEIEATRLMAWEAAWLIDQGR
                     DATREVYLAKITADDISLMCTDRAVQILGGHGYIRDHPVEMWLRNARGFATFEGMAMV
                     "
     gene            364..1584
                     /locus_tag="AC162G_07770"
     CDS             364..1584
                     /locus_tag="AC162G_07770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5075746.1"
                     /translation="MISFEIPESIAQEREQLQATAAGMMREQARYYDEHEHEIPWDYI
                     NMMWQAARASGRSYRGRGQDTGEGPRIGQQRMSHMVEMMSWGDAGLYLCTPGSGLGGA
                     AVEATGTPEQKEKFLARFADEKPAWSCMAMTEAHAGSDTAAIRTRAVKDGDEWVINGR
                     KIFVTCGHKALVDSQGFVVVWATIDPAAGRAGMRPFVVEAGTPGMTVTKLEYKMGIRA
                     SDTAELVLDNCRVPAENMLGSEEIATQQKGFKGAMATFDATRPLVAASALGIARATLD
                     LVKELLAAEGVTIPYGVPRTQLSAVQRDVLEMEAMIHAAWLLTLKATWLMDERKPNTI
                     EASMCKVKAGDVVTRVTQKGVELLGPLGYSRRLLLEKWMRDAKINDLFEGTGQINRLV
                     VARRILGYRSRELK"
     gene            complement(1636..3159)
                     /locus_tag="AC162G_07775"
     CDS             complement(1636..3159)
                     /locus_tag="AC162G_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5075747.1"
                     /translation="MSLRGFTVYDLLQHNALASGDAPAAISAGRTVSHREFLNRVDRL
                     AAGLTARGVAKGDRVCILAQNSIEYLELYGACAKTGAVAYPINWRLSASEVQAVVALA
                     DPQMIVVGANHMAQVEGIDLTGLRAGAIIGEEGGSATCPTGYVPLAEFYQSPAGSPAE
                     VNSNDPFVILSTAAVAGVPRGAVLTHANMILGGYQLITALGLTARDRHLAALPFFHVT
                     GLGLSLCMAQVGGANVVMESFDPALAAQWIDEHEATLIAVFPPVLSMLLEARDKVGVR
                     WQTLKHVVGLDAPDVIQRLLAETGAKFWTGFGQSETAGLLTLTRVDEKPGSAGKPLPL
                     ARVRLVNEAGDDVPVGEPGEIAAQGPLVFAGYWRDPDATDYTFRRGWHHTGDIGRFDA
                     ENYLYYVGRKPEKDLIKSGGENVYPAEVERVIQELPEVAAVCVIGVPHPKWGEAVKAV
                     VELKPGQALTAEQVSAAVADRIAAYKKPQSVNFVEKMPRQPDGEIDRTAVKAAHGRG"
     gene            complement(3175..3969)
                     /locus_tag="AC162G_07780"
     CDS             complement(3175..3969)
                     /locus_tag="AC162G_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5075748.1"
                     /translation="MLKINNVEVVYSDVILILHGVSIEVKKGQIVALLGANGAGKSTT
                     LKAISGLLRSQRGEVTRGSIEFKGQRIDRMRPDEIVRMGVVQVLEGHKLFQHLTVEEN
                     LLAGALVRSNGPEIRQDIEQVYHYFPLLKDLRHKVSGYLSGGEQQMLVIGRGLMGRPS
                     LMLLDEPSLGLAPLLVTEIFEVVQRINSEEGTSILLVEQNARMALEVANYGYVMENGR
                     VVLDGPAETLRENEDIKEFYLGLTSVGERKSYRDVKHYKRRKRWLG"
     gene            complement(4108..5490)
                     /locus_tag="AC162G_07785"
     CDS             complement(4108..5490)
                     /locus_tag="AC162G_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5075749.1"
                     /translation="MHKQVFAMLSVVVLVGIALAACAPAATATPTQAPPTTAPPTAVP
                     PTAEPPPTEAPPAVRRCADGLEGETIYYHSQAGLTGPLATILGTAFVNGLNDGIAEIN
                     ASGGICGATLELHLTDTQYEAEQEIAVYQQTKADFPNLTSINTYGSGATIALKDLLVE
                     DHVVNFAAGLNAEAFYNPPDGWTVGDYPIYPDQFGGFLQFVQDNWDDIKPDGAGDEII
                     VGVIGWEGPFGAGATTPEALAFAESLGITVLPLETQAVSAEADVVTPLQSLAVQGANV
                     IYIQSLGFGPAQVIGTLRALGMWDSVVVGGVNWAMNTDVLTFLGESAPAAVGMYAPFP
                     YRWWNDTDVPGVQMALAAFEAGGYPESDKGVSYLTSYAGLWGFKTIVEHAIDTVGYEN
                     LDGDAFFDAFKELGTVDNLGVWSFDVRDGSRAPHVAQIRQVQLVDGQPQFVVVKDFFE
                     LPDLRPSPAE"
     gene            complement(5606..6676)
                     /locus_tag="AC162G_07790"
     CDS             complement(5606..6676)
                     /locus_tag="AC162G_07790"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5075750.1"
                     /translation="MGVMRPSGDFDRSYQQDMSFLRKPWHWVLLFASIGAVATSPYWA
                     PPAFVIQANRIGYWIIAVQGLNILVGYTGQISLGQAAFMLVGGYVSTLCVTYLGFSFF
                     VALPIAALGAGLAGLIFGLPSLRVKGFYLAMATLAAQFIIPWLTRYTFPEYLGGTEGR
                     ISTPVPKIGDFAFDEPARYIYITLGVLIVTTVLAANISRSRLGRAFVSIRDSDLAAEL
                     LGVNLFGYKLRAFFIAAMYAGVAGALKAHSQRAVGTEFGYGLIESIVMLGMLVVGGLG
                     TNAGPFMGATFIIGMQEVSNWLGRTVAEAMPIEMTARLQTSFRPIFFGVVLMLFLIFE
                     PHGLARRWQLFKAAWRLRPFSR"
     gene            complement(6689..7624)
                     /locus_tag="AC162G_07795"
     CDS             complement(6689..7624)
                     /locus_tag="AC162G_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5075751.1"
                     /translation="MNWPLVPQAIVTGLLNGGVLGLIALGLVLIYKSSEVFNFAQGSL
                     VMLGAFVVWWFAGAQEKGGELFNLPLGFAVLAALVVAVWVGFLVERLTLRPMAGQPLL
                     SIILMTLGLAQFIEGVVSILFGIEPQGNFPKPFPPTESFNIPFDLAFGGAIHIQKTRV
                     VAFGVAIVGAILFASFFRLSRTGLAMRATAEDHELARSVGINVPRVFGLSWAIAGMIA
                     TAGGVLLAMLTGVSLSLSTVVLVAFPALLLGGVDSLGGAIVGGIAVGLAQEFVSASRL
                     IEIRNSSDIAPYVLLLIVLLIRPEGLFGQKRIERI"
     gene            complement(7621..9552)
                     /locus_tag="AC162G_07800"
     CDS             complement(7621..9552)
                     /locus_tag="AC162G_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5075752.1"
                     /translation="MDAVDTLPKCLVAARPKFGAHRVAMRQKDKGIWREYTWQQSYEQ
                     VRRLTLGLIRLGLQRGEKVCIIGDNDPQYFWTQLAIQAAGGVAVGIFTDSIPAEIQHI
                     VQHSDTVLVFAKDQEQCDKFLDIRDQIPAVRRVIYWDDKGLWSYSDPWLMSFEAVQAL
                     GQSLDAEQPDLFDRSVGEGQGDDLAVFCYTSGTTGQPKGAMISHSNLILGADQGVQID
                     PRGPTDEYVSFLPPAWITENALGLTYHLRFGVVVNFPEAPETVQENIREIAPHSLLFS
                     ARLWENLVALVQVRMAECSWLNRWLYRMFMPVGYKVAGLRFEQRQPVGLLWRLLHALG
                     EFAVFHPLRDKLGLTRVTSAYSSGAALSPDVIRFFRAIGVSIKQLYGSTEAQTHTLHI
                     GDDVKFETVGVPPPGVELRISDAGEIHVSSVTVFQGYYKNPEATARALFTDEEGRRWF
                     RTGDAGHFGPDGHLIYLDRVKELIELAGGQRYSPQYIEGRLKFNPYIRDVMTVGGADK
                     AFVTALINIDFENVGHWAEKRGLAYTTFVDLSQKPEVYELIRKEVDQVNRTLPPAARV
                     RKFVLLHKEFDADEGELTRTRKLRRDRCSERYRDMLVAMYDGGQEVRVQAEVRYRDGR
                     EGVVETTVHIMTLEESVTP"
     gene            complement(9554..10330)
                     /locus_tag="AC162G_07805"
     CDS             complement(9554..10330)
                     /locus_tag="AC162G_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5075753.1"
                     /translation="MAEDSVHLSVQDVSLSFGGTTALNDVSFDVCRGEILALIGPNGA
                     GKTSTLNCLNRFYEPDKGRIIFEGRDLRRVPRHRIAQWGIARTFQNLALYTGLSTLDN
                     LLAARHVHMRAGLLQCLLYWGAARREDIAHRQVVEDIIDFLEIEHIRKAIVGALPYGL
                     RKRVELGRALALEPRLLLLDEPMTGMNVEEKEDMARFILDIHERQKMTILLIEHDMGL
                     VMDIADRVVVLDFGVKIAEGKPDEIRHNPAVIKAYLGEVE"
     gene            10698..11723
                     /locus_tag="AC162G_07810"
     CDS             10698..11723
                     /locus_tag="AC162G_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011595351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR5075754.1"
                     /translation="MRLGINLGYSGATLNLPMDWIQEADRLGVYAVWTAEAYGSDCIA
                     PLAWIGALTKRIRLGTAIMQMPARTPANAAMTAMTLDQLSGGRFLMGLGMSGPQVVEG
                     WHGQAYGKPLARTREYVGIIRAIFKREKPLVHDGEHYQIPYRGADATGLGKPLKSTLH
                     GRPDIPIYLAAIGPKNVELAAEIGDGWLPIFFAPRHYDSAYKEHVEAGLAKAGKRPEQ
                     LDIAPSVIVALGEDVDACRSLVKPILALYIGGMGARGKNFYNDLACRYGYEAAAKEIQ
                     DLYLVGRKDEAIAAVPDALVDDVALCGPQARIAERLELWRQCPVTTLNITTFTIDGLR
                     AMVELVM"
     gene            complement(11754..13418)
                     /locus_tag="AC162G_07815"
     CDS             complement(11754..13418)
                     /locus_tag="AC162G_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HIR5075755.1"
                     /translation="MSQRHFAHWPRTVPRHLTAPETNLFYNVEVSATRYPNKPYLIYY
                     DTAISFAEFKDGAERLAGFLEHECGIKKGDRVLLFMQNSPQFVLAFYAILRANAVVVP
                     INPMNLTHELRSYVTDSGATTMVTSQELYPQVKPLISEGLKQVVLAAYSDYLRQATDL
                     RVPEFISAPRQNIRDAGVTLWSEALARNHRPGPLIAGPDDLCVMPYTSGTTGRPKGCM
                     HTHRTVMHTLVANVEWFQTNQDAVALAVLPFFHVTGMQGSMNSPLYSGSTVVLLPRWD
                     REVAGQLIQRYKVTGWTNIPTMIVDFLSNPKVTEFDLSSIMHVAGGGAAMPEAIAQRL
                     QDLCGLTYVEGYGLTETMAPTHINPPDRPKKQCLGIPIFDTEARVVDIETFRELPPGE
                     VGEIVVSGPQVFLGYWNNPRATEEAFVDMDGKRFFRTGDLGCIDEDGYFFIVDRLKRM
                     INAAGYKVWPAEVEVMMYQHPAIQEACINGVEDARRGETVKALVVLKPDAHGTVTEQD
                     IIDWAAQNMAAYKYPRIVEFVDTLPKSATGKIMWRALQEREKAQKT"
     gene            complement(13457..14677)
                     /locus_tag="AC162G_07820"
     CDS             complement(13457..14677)
                     /locus_tag="AC162G_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5075756.1"
                     /translation="MDFTYSERTQALQAKLNAFMDEYIYPSEEKFVAEVEENRRKGNA
                     WVPTKIMEELKEKARAQGLWNLFLPESEYGAGLTNLEYAPLCEIMGRSPIAPEAFNCG
                     APDTGNMEVLVRYGTAEHKKQWLEPLLEGKIRSAFAMTEPGTASSDATNIQARIVRDG
                     DHYVINARKWWASGGGDPRCKVLIFMGKTDPDNPDRHRQQSMIIVPMETPGVKVVRNL
                     PVFGYDDAPHGHAEMTFEDVRVPVSNILLGEGRGFEIAQGRLGPGRIHHCMRLIGLAE
                     RALEKMCKRTLKRVTFGRPVAEQTVTLERIANARILIDQARLLVLNAAHMMDTVGNKV
                     AAKEIAMIKVAAPHMACQVIDWAIQAHGGAGVCDDFGLASAYAHARSLRLADGPDEVH
                     RNTIGKLELRRYKQ"
     gene            complement(14735..>15273)
                     /locus_tag="AC162G_07825"
     CDS             complement(14735..>15273)
                     /locus_tag="AC162G_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR5075757.1"
                     /translation="QYRASETETIEAMDNLIDWLPQHIPADDETRIVHGDFRIDNVVF
                     HPREPRALAVLDWELSTLGHPMSDFAYHCMVWRMSPGEFRGLAGADVKALGIPSEAEY
                     VAAYCRRVGREPVPEHEWEYYMAFNMFRLAAILQGVLARALQGNASNQNALETGRRAR
                     ALSEAAWRQVERIMVERH"
BASE COUNT         2981 a         4729 c         4715 g         2848 t
ORIGIN      
        1 ggagcgcgaa gccttcggcg agccgatcgc acaacgtcaa tcgattgcgt tcatgttggc
       61 ggagatggca accgagatcg aagcgacgcg gctgatggcc tgggaagcgg cctggctgat
      121 cgaccaggga cgcgacgcga cgcgcgaagt ctatctggcc aagatcacgg ccgatgacat
      181 atccctgatg tgcacggatc gcgccgtgca gatcctcggc gggcacggct acatccgcga
      241 tcatccggtg gagatgtggc tgcgcaatgc gcgaggtttt gcgacctttg aaggcatggc
      301 gatggtgtag agaattggaa gtgatgaatc gggtcttccc gttgactcaa aggaggctca
      361 aagatgatct catttgaaat accggagtcg atcgcgcagg agcgcgagca gttgcaggcg
      421 accgcagccg ggatgatgcg cgagcaggcg cgatactacg acgagcacga gcacgagatt
      481 ccgtgggact acatcaacat gatgtggcag gcagcccggg cgagcggacg aagctaccgt
      541 ggccgaggtc aggacacagg cgaggggccg cgcatcggac agcaacgcat gagccatatg
      601 gtcgaaatga tgtcgtgggg ggacgccggg ctttacctgt gcacgccggg cagcgggctg
      661 ggcggcgcgg cggtcgaagc caccggcacg cccgagcaaa aggaaaagtt cctggcgcgc
      721 tttgcggacg agaaaccggc ctggtcgtgt atggcgatga ccgaagcgca cgccggctcc
      781 gacaccgccg ccatccgcac gcgtgcggtg aaggacgggg acgaatgggt gatcaatggc
      841 cggaaaatct tcgtgacgtg tgggcacaag gcgctggttg actcgcaggg attcgtcgtg
      901 gtgtgggcca cgatcgatcc ggcggcggga cgcgccggga tgcgcccgtt cgtcgtcgag
      961 gcaggcacgc cgggcatgac ggtgaccaaa cttgagtaca aaatgggcat tcgcgccagc
     1021 gacacagccg aactcgtcct ggacaactgc cgcgtcccgg ctgagaacat gctcggcagc
     1081 gaagagatcg ccacccagca gaagggcttc aagggcgcga tggcgacctt cgacgcaaca
     1141 cggccgctgg ttgccgccag cgccctgggc atcgcccgcg ccacgcttga tttggtgaaa
     1201 gagctactgg ccgccgaagg cgtcacaatt ccctacggcg tcccgcgcac acagctcagc
     1261 gccgtccagc gcgatgtgct ggagatggaa gcgatgatcc acgcggcgtg gctgctcacg
     1321 ctcaaggcga cctggctgat ggacgagcgc aagccgaaca cgatcgaagc gtcgatgtgc
     1381 aaggtcaagg ccggcgacgt ggtgacgcgc gtgacgcaga agggcgtgga gctgctcggc
     1441 ccgctcggct attcgcgccg gctgctgctg gagaagtgga tgcgcgacgc gaagatcaac
     1501 gatctgttcg agggaaccgg gcagatcaat cggctggtcg tggccaggag gattctgggc
     1561 tatcggagtc gagagttgaa atgaattgcg ggcgctgctt gcgccccgcc cagcggggtt
     1621 gagcgtctct tgctgtcagc cgcgaccatg cgcggccttg accgcagtgc gatcgatttc
     1681 gccgtcgggc tgcctcggca tcttttcgac gaagttgacg ctctgcggtt tcttataggc
     1741 ggcgattcgg tcggcaacgg cggcgctgac ctgctcggcg gtgagcgcct gacctggctt
     1801 caattcgacg acggccttca ccgcttcgcc ccacttggga tgtggcacac cgatgacgca
     1861 gacggctgcc acttccggca gttcctggat cacgcgctcg acttcggccg gatagacatt
     1921 ctcgccgccg gatttgatca gatccttctc cggcttgcgg cccacgtaat acaggtagtt
     1981 ttcggcgtcg aacctgccga tgtcgccggt gtgatgccag ccgcgccgaa aagtgtaatc
     2041 agtggcatcg ggatcgcgcc aatacccggc aaagaccagc ggcccctgcg cggcgatctc
     2101 gcccggctcg cccacaggca cgtcgtcgcc ggcttcgttc accaggcgaa cgcgcgccag
     2161 gggcagcggc ttaccggccg aaccgggctt ttcgtccacg cgggtcaacg tcaacagccc
     2221 ggcggtttca gattgcccga agcccgtcca aaacttggca ccggtttcgg ccagcaggcg
     2281 ctgaatgacg tcgggcgcgt ccaggccgac gacatgcttg agcgtctgcc acctgacccc
     2341 gaccttgtcg cgcgcctcca gcagcatcga cagcacgggt ggaaagacgg ctatcaacgt
     2401 cgcttcgtgc tcgtcgatcc actgcgccgc cagcgccgga tcgaagctct ccatgaccac
     2461 attcgcgcca ccaacctgtg ccatgcacaa cgacaggccc agcccggtga catgaaagaa
     2521 cggaagcgcg gccaagtgtc gatcgcgcgc ggtcaatcct aaagccgtga tcaactgata
     2581 gccgcccagg atcatattcg cgtgggtgag gaccgcaccg cgcggcacac ccgccacggc
     2641 agccgtcgag aggatcacga acggatcgtt gctgttcacc tcggccggcg agccggcggg
     2701 cgactgataa aactcggcca gggggacgta gccggtgggg cacgtcgctg atccgccttc
     2761 ctcgccgatg atcgcccccg cgcgcaggcc cgtcagatcg atgccttcca cctgcgccat
     2821 atgattggcg cccaccacta tcatttgtgg atcggccagc gcgaccaccg cctgaacttc
     2881 cgaagccgat aggcgccagt tgatcgggta cgcgactgcg cccgtcttgg cgcaggctcc
     2941 gtacagttcc aggtattcga tcgagttttg ggccaggatg cagacccgat cgcctttcgc
     3001 aacgccgcga gcggtgaggc ccgccgccag ccgatcgacg cggttgagaa actcccggtg
     3061 ggataccgtc cggccagcac tgatcgcggc cggggcgtcg cccgaggcga gtgcgttgtg
     3121 ttgaaggaga tcgtagacgg tgaagcctcg cagactcatg agcacacccc tttcttaccc
     3181 cagccagcgt ttacgccgct tgtagtgctt cacgtcgcgg tagctcttcc gctcgccgac
     3241 actggtcaac cccagataga attccttgat gtcctcgttc tcccgcagcg tctcggccgg
     3301 gccgtcgagt accacgcgtc cgttctccat cacgtagccg tagttcgcca cctccagcgc
     3361 catgcgagcg ttctgctcta ccagcaggat cgaggtgccc tcctcgctgt tgatgcgctg
     3421 gacgacttcg aagatctccg tgaccagcag gggagccagc ccgagcgaag gctcgtccag
     3481 cagcatcagg ctgggacgtc ccatcagccc ccggccgatg acgagcatct gctgttcgcc
     3541 gccggagagg tatccgctga ccttgtgccg cagatccttg aggaggggga agtagtggta
     3601 gacctgttcg atgtcctgcc gaatttcagg accgttggag cgcaccagtg caccggccaa
     3661 caagttttct tcgacggtca ggtgctggaa gagcttgtgt ccctcaagca cctggacgac
     3721 gcccatccgg acaatctcat cgggccgcat tctatcaatg cgttggcctt tgaactcgat
     3781 actgccacgg gtgacctcac ctcgctggga gcgcagcagg ccggaaatcg ctttcagcgt
     3841 cgtcgatttg cccgctccat tagcgcccaa gagcgccaca atctgtccct tcttgacttc
     3901 gatggagacg ccgtgcaaga tgaggatcac gtcgctgtag acgacttcga cgttgttgat
     3961 tttcagcatc gagaacctgc cgctggtacg gggttaggat tcgggaaaag ggggagcgcc
     4021 ccctgaacgc gctgcttgcg ccccgcctag cggggttgga ggagctccca ccttcttgct
     4081 cacaacacct cgtcgaaacg tgccacgcta ctcagccggc gacgggcgca agtcgggcag
     4141 ttcgaagaag tccttgacga cgacgaactg cggctggccg tccaccaact gaacctggcg
     4201 gatctgcgcc acatgcggcg cgcgacttcc atcgcggacg tcgaacgacc agacgccgag
     4261 attgtcgacg gtgcccagct ctttgaaggc gtcgaagaag gcgtcgccgt caagattttc
     4321 gtagccgact gtgtcgatgg cgtgctcgac gatggtcttg aacccccaca gtcccgcata
     4381 ggaagtcagg tagctgacgc ccttatcgct ctccggataa ccgccggctt caaaagcggc
     4441 cagcgccatc tggacgcccg gcacatcggt gtcattccac caccgatagg ggaagggcgc
     4501 atacatgccc actgccgccg gggcgctctc gccgaggaac gtcaggacat ccgtgttcat
     4561 ggcccagttc acgccgccga cgaccaccga atcccacatg ccgagggcgc gaagcgtgcc
     4621 gatcacctgc gccgggccga agccaagcga ctgaatgtag atcacgtttg cgccctgcac
     4681 ggccaagctt tgcagcggtg tgaccacgtc cgcctcggcc gatacggcct gcgtctccag
     4741 agggagaacg gtgatgccca gtgattcggc aaaggccagc gcctccggtg tggttgcccc
     4801 ggccccgaag gggccttccc aaccaatcac gccgacgatg atctcgtcgc cggcgccgtc
     4861 aggcttgatg tcgtcccagt tgtcctggac gaactgcagg aaaccgccga actgatcggg
     4921 gtagatcgga tagtcgccca ccgtccagcc gtccggcgga ttgtagaaag cctccgcgtt
     4981 gaggccggcg gcaaagttga cgacgtggtc ctcgacgagc aaatccttga gcgcgatggt
     5041 cgcgccactg ccgtaggtgt tgatagacgt cagattggga aagtcggctt tggtttgctg
     5101 atagaccgcg atttcctgct cggcctcgta ctgcgtatcg gtcagatgca attcgagcgt
     5161 cgcgccacag atgccgccgc tggcgttgat ctcggcaatc ccgtcattga ggccgttgac
     5221 aaaggcggtg ccgagaatgg tggccaacgg cccggtcaga ccggcttgcg agtggtagta
     5281 gatggtttcg ccttccaaac cgtcggcgca acgtcgaacg gctggcggcg cttcggtggg
     5341 cggtggctct gcggtcggcg gtactgcagt cggcggcgct gtggtgggtg gcgcttgggt
     5401 aggcgtggcc gttgcggcgg gtgcgcaggc agcgagggca atcccgacca ggaccaccac
     5461 ggacaacatt gcgaaaactt gcttgtgcat ggtttctctc ctccttagga tggaataacg
     5521 gggtaatcag actcccctca ttggcaagac ctagcggcga atacaggatt gagtcgaaat
     5581 gcatcacctc cttgtgatga tgtgtctaac gcgagaaagg cctcagacgc caggcagctt
     5641 tgaagagttg ccagcgacgg gccaggccat gcggctcaaa gatgagaaac aacatcagga
     5701 ccacgccgaa gaagatcggc ctgaacgaag tctgcagacg ggccgtcatc tctatgggca
     5761 tggcctccgc caccgttctc cccaaccagt tagagacttc ttgcatgccg atgatgaaag
     5821 tcgcccccat aaacggaccg gcgttcgtgc ccaatcctcc gacgaccagc atgcccaaca
     5881 tcacgatcga ctcgatgaga ccatatccga attcggtgcc caccgcgcgc tgggagtggg
     5941 ctttcaacgc gccggccacg ccggcgtaca tggcggcgat gaagaatgcg cggagcttgt
     6001 agccgaacag gttcacgccg agcagctcag ccgccaggtc gctatcgcgg atggagacaa
     6061 acgcccggcc cagacgcgat cgcgagatgt tggcggccag gacggtggtg acgatcagga
     6121 cgcccagcgt gatatagata tagcgggccg gttcgtcaaa ggcaaagtcg ccgatctttg
     6181 ggacgggagt ggagatgcgc ccctcggtgc cgcccagata ttccggaaaa gtgtatctgg
     6241 tcaaccacgg aatgatgaac tgggcggcca acgtggccat tgccaggtag aagcccttga
     6301 cacgcagcga gggcaatcca aaaatcaacc cggccagccc tgcccccagc gccgcaatcg
     6361 gcagggccac gaagaacgaa aagcccaggt acgtgacgca taaggtggag acgtatccgc
     6421 ccaccagcat gaaggccgcc tgccccagcg agatttgccc ggtatacccg accagaatgt
     6481 tcagtccctg cacggcgatg atccaatagc cgatacggtt ggcctgaatg acgaaagcag
     6541 gcggcgccca ataaggcgag gtcgcgacag cgccaatgga tgcaaacaac aagacccagt
     6601 gccagggctt tcgcaggaag gacatgtcct gttgatagct gcgatcaaaa tcgccagaag
     6661 gacgcataac gcccatgccc tattctcctt aaatcctttc gatacgcttc tgtccgaaca
     6721 gcccttcagg ccggatgagt aggacgatga gcagcagcac gtagggcgcg atatccgacg
     6781 agttgcgtat ctcgatcagt ctggatgcac tgacaaattc ctgcgccagc cccacggcga
     6841 taccgcccac gattgcgccg ccgagtgagt ctacgccgcc caaaagaagc gctggaaaag
     6901 ccaccaacac aacggtcgac aggctgagac tgacaccggt cagcatcgcc aagagcaccc
     6961 cgcctgccgt ggcaatcatt ccggcaatcg cccacgaaag tccgaagaca cgcgggacgt
     7021 tgatgccgac gctgcgcgcc agttcatggt cttccgccgt ggcgcgcatc gccaggccgg
     7081 tgcggctgag tctgaagaac gaggcaaaca agatcgcgcc cacgatcgcc acgccaaagg
     7141 ccactacgcg cgtcttttga atgtgaatcg ccccgccgaa ggccagatca aacgggatat
     7201 tgaaagactc ggtgggcggg aagggcttgg gaaagttacc ttgaggctcg atgccaaaga
     7261 gaatgctcac gacaccctcg atgaattgcg ccaggccaag cgtcatcaag ataatcgaca
     7321 gaaggggttg ccccgccatg gggcgcaggg taagccgctc gacgagaaag ccaacccata
     7381 cggccaccac cagggcggca aggacggcaa accccaacgg cagattgaat aactcaccgc
     7441 ccttttcctg ggcaccggcg aaccaccaca ccacgaatgc gccgagcatc accagagagc
     7501 cttgcgcgaa gttgaagact tcggatgatt tatagatcag caccaggccc agcgcgatga
     7561 gtcccagcac cccgccgttg agaagaccgg ttacgattgc ctgcggtaca agcggccagt
     7621 tcatggtgtg actgactcct ccagtgtcat gatgtggacg gtcgtctcga caaccccctc
     7681 acgcccgtcg cggtaacgca cctcagcctg cacgcgcact tcctggccgc cgtcgtacat
     7741 cgccaccagc atgtcccggt agcgttcgct acaccggtct cgccgcagtt tgcgggtccg
     7801 agtcagctca ccttcatcgg catcgaactc cttgtgcagc agcacgaact tgcgcacgcg
     7861 cgccgctggc ggcaaggtgc gattgacctg gtcgacctct ttccggatca attcgtagac
     7921 ctccggtttc tgagagaggt ctacgaaagt cgtgtaggcc aggccgcgct tctcggccca
     7981 gtgaccgacg ttttcaaagt cgatgttgat caaggccgtc acgaaggctt tgtcggcccc
     8041 gcccaccgtc atgacgtcgc ggatataagg gttgaacttg agtcgccctt cgatatactg
     8101 tggcgaatat ctctggccgc cggccagctc gatcaattct ttcacacgat cgagatagat
     8161 cagatgaccg tctggcccga agtgaccggc gtcgccggtg cgaaaccagc ggcggccttc
     8221 ctcgtcagtg aacaaggctc tggcggtggc ttcgggattc ttgtaatagc cttgaaagac
     8281 tgtcacacta ctcacgtgaa tttcgccggc atcggagatg cgaagctcca cgccgggagg
     8341 cggcacgcct actgtctcga acttcacgtc gtctccgatg tgcagggtgt gcgtctgcgc
     8401 ctcggtcgag ccgtacagtt gcttgatgct gacgccgatg gcgcggaaaa agcgtatgac
     8461 atccgggctg agcgccgcgc cggacgaata ggccgacgta acacgggtca gtcccaactt
     8521 gtcgcgcagg gggtggaaga cggcgaactc tcccaacgcg tgaaggagcc gccagagcag
     8581 ccccaccggt tggcgctgct cgaatcgcag gccggccacc ttgtatccga cgggcataaa
     8641 catcctgtac aaccagcggt tcagccatga acactcggcc attctgactt gcaccaacgc
     8701 caccaggttt tcccacaggc gggcgctgaa gagcagcgaa tggggggcga tttcgcgaat
     8761 attctcctgc accgtttccg gcgcttcggg aaagttgacc accacgccga atcgcaagtg
     8821 atacgtcaga cccagagcgt tttcagtgat ccaggccggt ggcaggaacg acacatattc
     8881 atctgtcggc ccgcgcggat cgatctgcac accctggtcg gcgccgagga taagattcga
     8941 gtggctgatc atggccccct tcggctggcc ggtggttccc gaggtgtagc agaataccgc
     9001 caggtcgtcg ccctgccctt cgccgacgga acggtcgaac agatcgggct gttccgcgtc
     9061 gagcgactgc cctagggcct gcacggcttc gaagctcatc agccacggat cgctgtaact
     9121 ccacaggccc ttgtcgtccc agtagatcac gcggcgtacg gccggaatct gatcccggat
     9181 gtcgaggaat ttgtcgcact gctcctggtc tttcgcgaag accaacacgg tatccgagtg
     9241 ctggacgatg tgctggatct cggcagggat gctgtcggtg aaaatgccaa ccgcgacccc
     9301 gcccgccgcc tggatggcca actgcgtcca gaagtattgc gggtcattgt cgccgatgat
     9361 gcacactttt tcaccgcgtt gcaggcccag gcggatcaag cccagtgtca agcgccttac
     9421 ctgttcgtac gattgctgcc aggtatactc gcgccagatg cccttgtctt tctggcgcat
     9481 ggcgacccta tgagcgccga atttggggcg ggcagccacc aggcacttgg gcagggtatc
     9541 tacggcgtcc atatcactcc acctccccca ggtaagcctt gatcacagcc gggttgtgcc
     9601 ggatttcgtc cggtttcccc tcggcgatct tcacgccgaa gtccagcacg accacccggt
     9661 cggcgatgtc catgaccaac cccatgtcat gctcgatgag caagatcgtc atcttctggc
     9721 gctcgtggat gtccaggatg aagcgggcca tgtcttcctt ctcctcgacg ttcatcccgg
     9781 tcatcggctc gtcgagaagg agcaagcgag gctccagcgc cagcgcccga cccagttcca
     9841 cgcgcttgcg caggccgtag ggcagcgcgc cgacgatcgc cttgcggatg tgctcgattt
     9901 caaggaagtc gatgatgtct tccacgacct ggcgatgggc gatgtcttcg cggcgggccg
     9961 ccccccagta gaggagacat tgaagcaggc cggcgcgcat atgaacatgg cgcgccgcca
    10021 acaggttgtc cagtgtgctg agaccggtgt acagggcgag attctgaaag gtgcgcgcga
    10081 tgccccactg ggcgatgcga tgtcgcggca cgcgtctgag gtcgcgtcct tcgaaaataa
    10141 tccggccttt atcgggttcg tagaagcggt tgaggcagtt cagcgtcgag gtcttgcccg
    10201 cgccgttggg gccgatgagg gcaaggattt cgccacggca aacgtcgaag ctgacgtcgt
    10261 tgagcgccgt ggtgccaccg aaactgagtg aaacatcctg cacggacagg tgaacggagt
    10321 cttcggccat gtgtgtccta cattagagac acttcgttct gtcgcttcgg atggcggaga
    10381 gggccaggat atacttcgtc agcacctctt gcaagactaa gtgatctgtt ttcgtgcgat
    10441 atgaaaggct gaggccaacc cttacttcaa gcgcacctcg cgctcgaggt agccattcag
    10501 gtaagcgacg gcccgattga cataggccct catttcagac cgagcggtct gatagtgaca
    10561 ggatactcgc gcagaggcga gttgtcaagg ttccgattct gtacgttctg tacaccgccg
    10621 ccacaactgc cgggtcaggc aagcgcgatt ttgcacatcg acccgactgt gtcacaattg
    10681 ccagggcgag gtggacaatg agactcggga tcaatcttgg ctattctggc gcaaccctca
    10741 acctgccgat ggactggatc caggaagccg atcgcctggg cgtgtatgcg gtgtggacgg
    10801 ccgaagcgta cggctcggat tgcatcgcgc ccctggcgtg gatcggcgcg ctcacgaaac
    10861 ggatccgtct cggcacggcc atcatgcaaa tgccggcgcg cacgccggcg aacgcggcga
    10921 tgacggcgat gacgctggat cagctgtcgg gcggacgttt tctgatggga ttgggcatgt
    10981 ccggcccgca agtcgtcgag ggctggcacg gtcaggcgta cggcaagccc ctggccagaa
    11041 cccgcgaata cgtcggtatt atacgcgcga tcttcaaacg cgagaagccg ctcgtccacg
    11101 acggcgaaca ttatcagatc ccctatcgcg gcgctgatgc gaccgggttg ggcaagccgc
    11161 tgaagagtac gctgcacgga agacccgaca ttccgatcta cctggccgcg atcgggccga
    11221 agaacgtcga gctcgccgcc gagatcggcg acggctggct gccgatcttt ttcgcgccac
    11281 gccactacga ctcggcctac aaggagcacg tcgaggccgg gctggcgaag gctggtaagc
    11341 gtcctgaaca acttgatatc gcgccgtccg tcatcgtcgc gctgggcgaa gatgtcgatg
    11401 cctgccggag tctcgtcaag cctatcttgg cgctgtacat cggtggcatg ggcgcgcgcg
    11461 gcaagaactt ctacaacgac ctggcctgcc gttacggcta cgaggcggct gcgaaagaga
    11521 ttcaagacct ttatctcgtt ggccggaaag acgaagccat cgccgccgtg cccgatgcgc
    11581 tcgtcgacga cgtcgcgctg tgcggcccgc aggcgcgcat cgccgaacgg ctagagctgt
    11641 ggcgccagtg cccggtcacc acactcaaca tcacgacgtt cacgatcgac ggactgcgcg
    11701 cgatggtcga gctggtgatg tgaacatcac cggtgaacgt tcgtatcgcc aactcaggtc
    11761 ttctgcgctt tctcgcgctc ctgcagcgcc cgccacatga tcttgccggt agccgacttg
    11821 ggcagagtgt ccacgaactc tacgatacgc ggatatttgt aggccgccat gttctgggcc
    11881 gcccagtcga tgatgtcctg ctcggtgacc gtgccgtggg cgtctggctt gagcacgacc
    11941 agcgctttga cggtctcgcc acgacgggca tcctcgacgc cgttgatgca agcctcctgg
    12001 atcgccggat gctggtacat cataacctcc acttccgccg gccagacctt gtaaccggcg
    12061 gcgttgatca tgcgcttcag gcgatccaca atgaagaagt aaccgtcctc gtcgatgcag
    12121 cccaggtcgc cggtacggaa aaagcgcttg ccgtccatgt cgacgaaggc ctcctcagtc
    12181 gcgcgcgggt tgttccagta accgaggaat acttgggggc ctgaaaccac gatctcgcct
    12241 acctcgcccg gcggcagctc gcggaaggtc tcgatatcca ccacgcgcgc ctcggtgtcg
    12301 aagataggaa tgccgaggca ttgttttttg ggtcgatcgg gcggattgat gtgggtcggc
    12361 gccatggtct ccgtaagccc gtagccctcg acgtaggtga ggccgcacag gtcctgcagg
    12421 cgctgggcga tggcttcggg catcgcagcg ccgccgccgg ccacgtgcat gatgctggat
    12481 agatcgaatt ccgtgacttt ggggttgctc agaaaatcca cgatcatcgt cggaatgttg
    12541 gtccagccgg ttactttgta acgctggatc agctggcccg ccacctcgcg gtcccagcgc
    12601 ggcagcaaga ccacggtgct gccgctatac aggggactat tcatactgcc ctgcatgccg
    12661 gtgacatgaa agaatggtaa caccgccagt gccaccgcgt cctggttggt ctggaaccac
    12721 tcgacattcg ccaccagcgt gtgcatcacc gtgcggtgag tatgcatgca gcctttggga
    12781 cgacccgtag tgccggaagt atagggcatg acgcacaaat catccggccc ggcgatcagc
    12841 ggcccggggc ggtgattgcg tgccagagcc tcgctccaca aagtcacgcc cgcatcccgg
    12901 atgttctgtc gtggcgcgct gatgaattcg ggcacgcgca ggtcggtcgc ttgcctgagg
    12961 tagtccgaat aggcagccag caccacttgc ttgagaccct cgctaatgag cggcttcacc
    13021 tgcggataca gttcctggga agtcaccatc gtggtggccc cgctgtcggt cacgtaagag
    13081 cgcagctcgt gggtgaggtt catgggattg ataggcacca ccaccgcatt ggcgcgcagg
    13141 atggcgtaaa aggccagcac gaattggggg ctgttttgca tgaacagcag cacccggtcg
    13201 ccctttttga tgccgcactc gtgctccaga aatcccgcca gacgttcggc cccatccttg
    13261 aattcggcga agctgatcgc cgtgtcgtag taaatgaggt agggcttgtt cgggtagcgc
    13321 gtggccgaga cttccacgtt gtaaaacagg ttggtctcgg gcgccgtcag gtgacgcggt
    13381 acggttctgg gccaatgggc gaaatgtcgt tgcgacacgg ctcttcctcc ccttatcctc
    13441 cctgattgga aagcccctac tgcttgtagc ggcgcagctc cagcttgccg atggtgttgc
    13501 gatgcacctc gtccggtccg tcggccaggc gtagcgaacg cgcgtgcgcg taggccgagg
    13561 caagtccgaa gtcgtcgcac acgccagcgc cgccgtgggc ctgaatggcc cagtcgatga
    13621 cctggcaggc catatgggga gccgccacct tgatcatggc gatttccttg gccgcgactt
    13681 tgttgcccac cgtatccatc atgtgggcag cattgagtac caggaggcgc gcctgatcga
    13741 tgaggatgcg ggcgttggcg atgcgctcca gtgtcaccgt ctgctccgcc acgggacggc
    13801 cgaaggtcac gcgcttcaga gtacgcttgc acatcttctc gagcgcccgc tcggccaggc
    13861 cgatgaggcg catgcagtgg tgaattcgac ctggccccag acgtccctgc gcgatctcga
    13921 aaccgcgccc ctcgcccagc aagatgttgg aaaccggcac gcgcacgtct tcaaaggtca
    13981 tctcggcgtg cccgtggggc gcgtcgtcgt agccgaacac tggcaggttg cgcaccactt
    14041 tgacgccggg ggtttccata gggacgatga tcatggactg ttgcctgtgc cgatcgggat
    14101 tatccggatc ggtcttgccc atgaaaatga gcaccttgca gcgcggatcg ccgccgcccg
    14161 acgcccacca tttgcgagca ttgatgacgt aatgatcccc atcgcgcacg atgcgcgcct
    14221 ggatgttggt cgcgtccgac gaggccgtgc caggctcggt catggcgaag gcggagcgga
    14281 tctttccttc cagcaagggt tccagccatt gcttcttatg ttccgccgtg ccgtaacgca
    14341 ccaacacttc catgttaccg gtgtcggggg cgccgcagtt gaacgcctcg ggcgcaatgg
    14401 gggagcgtcc catgatctcg cataggggcg catactccag gttggtcagc cctgcgccgt
    14461 attccgactc aggcaagaac aggttccata gaccctgtgc gcgcgccttt tccttcagtt
    14521 cctccatgat cttcgtcggc acccaggcgt tgcccttgcg gcgattctcc tccacctcgg
    14581 cgacgaactt ttcttcgctc ggatagatgt attcatccat gaaggcgttg agctttgcct
    14641 gcaacgcttg agtgcgttcc gaatacgtga agtccattgg ccttcctcct gtcggtcggc
    14701 ggatgtgggt cgacggcgaa agcgcctgcc gcgctcaatg cctctcgacc atgattctct
    14761 ccacttgacg ccaggctgcc tcgctgagag cacgcgcgcg gcgacctgtc tccagggcat
    14821 tctggttcga ggcgtttccc tgcaacgctc gcgccagcac cccctgcaaa atggcggcga
    14881 gccgaaacat gttgaacgcc atgtagtatt cccattcatg ctccggcaca ggctcgcgcc
    14941 ccacacggcg acaataggct gccacgtatt ccgcctcgct tggaataccc agagccttga
    15001 cgtccgcgcc cgccagacca cggaactcac ccggcgacat gcgccaaacc atgcagtggt
    15061 aggcgaagtc ggacatggga tgccccaggg tggacaattc ccaatcgagc accgccaggg
    15121 cgcgcggctc cctgggatgg aaaacgacgt tgtcgatgcg gaaatcgccg tgcacgatgc
    15181 gggtctcgtc atcggcggga atatgctgcg gcaaccagtc gatgagattg tccatggcct
    15241 cgatggtctc ggtctccgag gcgcgatact gtt
//
LOCUS       DBNHXB010000193         4526 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1889091, whole genome shotgun sequence.
ACCESSION   DBNHXB010000193 DBNHXB010000000
VERSION     DBNHXB010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1889091"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="AC162G_07830"
     CDS             complement(<1..571)
                     /locus_tag="AC162G_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016679 - oxidoreductase activity, acting
                     on diphenols and related substances as donors [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HIR5075758.1"
                     /translation="MRDIKIEWLRVAVHAGALLPLALLIWDASQNALSVNPIQDVTFR
                     TGRTALALLVLTLGCTPLNTLFGFKKALAVRRALGLYAFAYAGLHLFTFAYLDYGLDL
                     ELIAQTIAEKRYVVVGFAAFLILLLLALTSTKGWQKRLGRRWKRLHRLSYVAGVLAVI
                     HFVWLVKSDITEPVRFGLVVALLLALRIPI"
     gene            complement(571..1515)
                     /gene="msrP"
                     /locus_tag="AC162G_07835"
     CDS             complement(571..1515)
                     /gene="msrP"
                     /locus_tag="AC162G_07835"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HIR5075759.1"
                     /translation="MTKIRSSEITPEHVYLSRRRFMAGAGTLAASAALAACGAQAPGA
                     AAPQPAPTGLSTQAQTDELGDPSNAYQAITNYNNYYEFTTDKQGVALLARSFKSEPWK
                     VEVGGLVNNPKTYDIDDLRKKFTQEERVYRLRCVEGWSMVIPWIGFPLASLLKEVEPT
                     STAKYVRFESILDPQQMPGQLNTWYSWPYVEGLRLDEAMNDLALLVTGLYGKPLPNQN
                     GAPLRLAVPWKYGFKSIKAIVKIDLVEEQPVSLWMRAASHEYGFYSNVNPEVPHPRWS
                     QATERRIGESGRRRTLMFNGYADRVADLYGGMDLRINY"
     gene            complement(1599..2456)
                     /gene="lgt"
                     /locus_tag="AC162G_07840"
     CDS             complement(1599..2456)
                     /gene="lgt"
                     /locus_tag="AC162G_07840"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR5075760.1"
                     /translation="MICEIAGRPAICIGPIPINFYGIIIVVGALVGGYVAATQARLNG
                     DDSDVVWDGLFWVVLAGIIGARLYHVFSTPAGCTADLPYCGWPWYQNHPQDIVLGIRE
                     GGLGIFGAVLGGFVAVVIYARRRRLPLARYIDYAAPSLLIGQAIGRWGNFVNRELYGP
                     PTDLPWGLSIDAAHRTYEYVDLTRYPEEVTRFHPLFLYESTLNVLGFIVLIVASRRLT
                     GRLKAGDLFLAYLIWYGLVRIFVESFRFDAWTSLGGIPTASLISIVLAVGALAALIVR
                     HRPRAGRTA"
     gene            3008..3730
                     /locus_tag="AC162G_07845"
     CDS             3008..3730
                     /locus_tag="AC162G_07845"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR5075761.1"
                     /translation="MLKHRITATLILALVLLVASLGTVAAEDGGAVAGVETSGDTALA
                     RGVVLRQALLQDETPTSTATPTPTATATLTPTATPTPTSTATLTPTATPTLTSTGTLS
                     PTATPEFCHPVARMIAQFFDLTCAEVTELHDSGVGFGVIARAYLTAQALDDEEVSPED
                     ILALHQSGMGWGKIMQEYGAAAHPGGKGLGTIMRDKSPDEDDEEGGASPDRLRPGNSG
                     NAPGHNKGNGPRGPGRGKGRPW"
     gene            4360..>4526
                     /locus_tag="AC162G_07850"
     CDS             4360..>4526
                     /locus_tag="AC162G_07850"
                     /inference="COORDINATES: protein motif:HMM:NF019428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HIR5075762.1"
                     /translation="MTCKKKLTDLHEESGQALVEFALVLMFVILPFTFVLIDGAMTLF
                     SQAAITNAAREG"
BASE COUNT          877 a         1369 c         1452 g          828 t
ORIGIN      
        1 caatcgggat gcgcagcgcc agcagcagcg cgacgacgag tccgaaccgg accggctcgg
       61 tgatgtcgga cttgaccagc cacacgaagt gaatgacggc cagcacgccg gcgacgtacg
      121 acaggcggtg cagccgcttc cagcgccggc ccaggcgttt ctgccagccc ttcgtcgacg
      181 tgagcgccag cagaagcaag atcagaaacg cggcgaagcc aacgacgaca tagcgcttct
      241 cggcgatcgt ctgcgcgata agctccagat ccaggccgta gtccaggtag gcaaatgtga
      301 acaggtgcag gcccgcgtac gcaaacgcgt agaggccgag cgcgcggcgc acggcgagcg
      361 ccttcttgaa tccgaacagc gtgttgagcg gcgtgcagcc gagcgtcaac accagcaggg
      421 ccagcgccgt gcggccggtg cgaaaggtga cgtcttgaat cggattgacg ctgagcgcgt
      481 tctgcgacgc gtcccagatc aggagcgcga gcggaaggag cgccccggcg tggacggcga
      541 cgcgcagcca ctcgatttta atgtcgcgca tcagtagtta atcctcaagt ccatgcctcc
      601 gtacaagtcc gcaacccggt cggcgtatcc gttgaacatc agtgtccggc ggcgacccga
      661 ttcgccgatg cggcgctcgg tggcctgcga ccagcgcggg tggggcacct ccgggttgac
      721 gttggagtag aagccgtact cgtgcgacgc ggcccgcatc cacaacgaaa cgggctgctc
      781 ttcgaccagg tcgatcttga cgatggcttt gatactcttg aagccgtact tccacggcac
      841 ggccagtcga agcggcgcgc cgttctggtt cggcagcggc ttgccgtaca ggccggtcac
      901 cagcagcgcg agatcgttca tcgcctcgtc caggcgcagc ccctcgacgt agggccaact
      961 gtaccacgtg ttgagctgac cgggcatctg ctgcggatcg aggatcgact cgaagcgcac
     1021 atacttcgcc gtcgaggttg gctcgacctc cttcagcagc gacgccagcg ggaagccgat
     1081 ccacgggatc accatcgacc agccctcgac gcagcgcagc cggtagaccc gctcttcctg
     1141 cgtgaacttc ttgcgcagat cgtcgatgtc gtaggtcttg gggttgttga ccagcccgcc
     1201 gacctccacc ttccacggct cggacttgaa gctgcgcgcc agtagcgcca cgccctgctt
     1261 gtcggttgtg aactcgtagt agttgttgta attggtgatc gcttgatacg cattgctcgg
     1321 atcgcccagc tcgtccgtct gggcttgcgt actcaggccg gtcggcgcgg gctgcggcgc
     1381 ggcagccccg ggcgcctgcg cgccgcacgc ggccagtgcc gccgaggcgg ccagcgtccc
     1441 ggcaccggcc atgaacctgc gacgcgacag gtagacgtgc tcaggcgtaa tctcggaaga
     1501 tcggatcttc gtcatcttaa cccaccatga cagaggtagg ggcaggcctt gcgcctgccc
     1561 aggtggtttg attgtattca ccacagatta cagggttctt acgcggttcg acctgcgcgc
     1621 gggcgatgcc ggacgatcag cgcggcgagc gcgcccacgg caaggacgat ggagatcagg
     1681 ctcgccgtgg ggatgccgcc cagcgacgtc caggcgtcga agcggaacga ctcgacgaag
     1741 atgcgcacca gcccgtacca gatcaggtac gccagaaaca ggtcgcctgc cttcaagcgt
     1801 cctgtgaggc ggcggctggc gacgatcagc acgatgaagc cgagcacgtt gagcgtcgat
     1861 tcgtacagga acagcggatg gaagcgcgtg acttcctccg ggtaacgggt caggtcgacg
     1921 tactcgtagg tgcggtgggc ggcgtcgatg gagaggcccc acggcaggtc ggtcggcggg
     1981 ccgtacagct cccgattcac aaagttgccc cagcggccga tagcctggcc gatgagcaga
     2041 gagggcgcag cgtagtcgat gtatcgcgcg agcggcaggc gcctgcggcg cgcgtaaatg
     2101 acgaccgcga cgaagccgcc gaggaccgcg ccgaagatgc ccagcccgcc ctcgcggatg
     2161 cccagcacga tgtcctgcgg atggttctgg taccacggcc agccgcagta cggcaggtcg
     2221 gccgtgcagc cggcgggtgt cgagaagacg tggtatagcc gggcgccgat gatgcccgcc
     2281 agcacgaccc agaacaagcc gtcccagacg acgtccgagt cgtcgccgtt caggcgtgcc
     2341 tgtgtcgccg cgacgtagcc gcccacgagc gcgccgacga cgatgatgat gccgtagaag
     2401 ttgatgggta tcgggccgat gcaaatggcg ggccggcccg caatctcgca gatcatgtgc
     2461 tcactcctgg ctgtgggtgg gcgggattat accatttttg gcgtaactgc tcggccgtca
     2521 ttgcgagaaa tccatcactc tggcgtgatg tgtaactgga gaaactcttc cggccaatgt
     2581 catgccgaga agtccatacc gccagtggta tgcgtgcgga cagaaacgcc cggccgtgtc
     2641 atgctgagca tcttttcgcg aagcatctct accctggcgt gccgtcctag ccggttgtgg
     2701 cagagattcc tcactgcgtt cggaatgaca cggtaatgac attggctgag tagttatttt
     2761 tggctaattg atacgcgtta ttcgggttat tcacggattg gtaaggtacc aatctcccaa
     2821 tttgtgcacg gagcgtatcc gcaccgcctg tcattcgttt tatctacata gaaggtcacc
     2881 ctgcgaaggt ttcaaacatg ccagtcggtg tgcacaccag gtagacaaca tcagtcgtgt
     2941 cgctcagttt ctccggaacc ttcgcaaggg tgagcagtaa ccaatctcgc acgatatgag
     3001 gtgagccatg ctcaaacatc gcataaccgc tacgttgatc ctcgcgctcg tcctgctggt
     3061 cgcatctctc gggacggtcg cagccgagga tggcggcgca gtcgccggcg tcgagacatc
     3121 gggtgacact gccctggcca ggggggtcgt cctccggcaa gcgctcttgc aggatgagac
     3181 acccacctcg acggccacgc cgacgccgac agcgacggct acgttgacac cgacggctac
     3241 tccgacgccg acatcgaccg ctacgttgac cccgacggcc acgccgacgc tgacatcgac
     3301 cggtacgttg agcccgacgg ccacgccgga gttctgccat ccggtggcgc gcatgatcgc
     3361 gcagttcttc gatctgacgt gcgcagaggt caccgagctg cacgacagcg gcgtgggctt
     3421 cggcgtgatc gcgcgggcgt atctcaccgc gcaggcgctc gacgacgagg aagtctcgcc
     3481 tgaggacatc ctggcgcttc accagtcggg tatggggtgg ggcaagatca tgcaggagta
     3541 cggcgccgct gcccatccgg gcggcaaggg tctgggcacg atcatgcgcg acaagtcgcc
     3601 ggatgaggac gacgaagaag gaggcgcgtc gcccgatcgt ctccgacccg gcaacagcgg
     3661 aaacgcaccc ggccacaaca agggcaacgg gccgcgcggc cccggtcgcg gcaagggcag
     3721 gccctggtaa tagtcctgtg gtctggtggt ccagtggtct cggtagatca cgatttcgta
     3781 gtgacgactt cagtcgtcgt tgagacttcc gaagtctgag agacttcgga agtctgaaga
     3841 taagcgtaga agcgccggtc acgaccggcg cttctgcatt cacacgccag actcggtaga
     3901 tcgtaactac tcagccgtca gtgcgacccc gctgagattg cttcgcaaaa agacgctcgc
     3961 aatgacgtgc ggggcaggtg agcgttgtgt gcgacgaagc agtctcctgc acggacggcg
     4021 atgctcgctg atgggtgctg agattgcttc gcaaaagacg ctcgcaatga cgcgggctga
     4081 gtagttacgg tagatccaat tttcgcggag cgaggcatgc tgcatgccga ttcgcgtcgc
     4141 acgtgtgacg gtatcattgc aggttcattt ccaattcgac tcgagacgca agacattgtc
     4201 gcaatccgca tactcttctg accgtgtgca caccgaagcc ggcgagagcg gatatctgat
     4261 cggcaaacct aaccgattct ttccggtttt cttactgaaa tcttatgcaa ttgccatatt
     4321 cttcgagcgt gttgggcggt atatgggtgg cgatagccta tgacatgcaa gaagaaactc
     4381 actgacctgc acgaagaatc aggtcaggcg ctggtggaat ttgcgctggt cttgatgttc
     4441 gtgatcctgc cgttcacgtt cgtattgatc gacggggcga tgacgctgtt ctcgcaggcc
     4501 gcgatcacga acgcggcgcg cgaggg
//
LOCUS       DBNHXB010000194         6171 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_43741, whole genome shotgun sequence.
ACCESSION   DBNHXB010000194 DBNHXB010000000
VERSION     DBNHXB010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6171)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_43741"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="AC162G_07855"
     CDS             <1..146
                     /locus_tag="AC162G_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HIR5075763.1"
                     /translation="DLGPRIAQFLLPIPGKRDSDWGYSWIPVVGPAIGGVLAAFLAQA
                     LGM"
     gene            182..1183
                     /gene="dhaK"
                     /locus_tag="AC162G_07860"
     CDS             182..1183
                     /gene="dhaK"
                     /locus_tag="AC162G_07860"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HIR5075764.1"
                     /translation="MKKLINKPEDVVKEELQGIAAAHPDLVKVFFEPNYIVRADAPVK
                     GKVGVISGGGSGHEPMHGGFVGPGMLDAACPGAVFTSPTPDQMLEATKAVNGGAGVLH
                     VVKNYTGDIMNFEMAADLARGEGIEVESVVTNDDVAVQDSLYTAGRRGVGVTVLSEKI
                     SGAAAEQKRSLKEVADICRKVNANGRSMGMALTSCTVPHVGKPTFDLPEDEMEIGIGI
                     HGEPGRTRMKLKTAAEITEMLAIPVLDDLPFKSGDEVLAFVNGMGGTPVIELYVVYNE
                     LAKICAGRRVKIVRNLIGSYITSLEMAGCSITLLKLDDNLTKLWDAPVKTAGMRWGM"
     gene            1276..1920
                     /gene="dhaL"
                     /locus_tag="AC162G_07865"
     CDS             1276..1920
                     /gene="dhaL"
                     /locus_tag="AC162G_07865"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017316502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HIR5075765.1"
                     /translation="MTNSVSRDAVLAWIRAYAAVIADNKEYLTQLDAAIGDADHGANM
                     DRGFQAALTKLPGVSDKDIGAILKTVGMTLVSTVGGAGGPLYGTLFMQMGTSSAGKLE
                     LTLADWVDALEAGVNGVVMRGKANLGDKTMVDALSPALVTLKEAAANGTPMREALVKS
                     AQAAEQGMIATIPLVAKKGRASYLGERSAGHQDPGATSSHLLLKAAADTWTNGG"
     gene            2002..3501
                     /gene="glpK"
                     /locus_tag="AC162G_07870"
     CDS             2002..3501
                     /gene="glpK"
                     /locus_tag="AC162G_07870"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIR5075766.1"
                     /translation="MAKYAAAIDQGTTSTRFMIFDHGGKVVEVDQREHEQIYPKPGWV
                     EHDPMEIWQRTQEVVKEALTKSKIDPKDISAIGVTNQRETTIVWEKATGKPVYNAIVW
                     QDTRTAGICNDLAKDGGQDRFRPKTGLPLATYFSGPKIKWILDNVENTRARAEKGELL
                     FGNIDTWVIWNVTGGPKGGAHVTDVSNASRTMLMNLETLDWDDEILKILNIPRAMLPK
                     VRPSSEVYGTAVGDLAGVPVAGDLGDQQGALFGQTCFSPGEAKNTYGTGCFMLLNTGT
                     KPVPSKSGLLTTLGYKIGDQPAVYCLEGSIAITGALVQWLRDNLGMIQKSADVEALAK
                     TVEDNGGIYFVPAFSGLFAPYWRNDARGVIVGMTRYVNKGHIARAALEATAFQTREVL
                     DAMNKDSGVPLKALKVDGGMVYNELLMQFQADLLGVPVIRPTVAETTALGAAYAAGLA
                     VGFWRNVEDLRANWAKDKEWQPKMDVEHREKLHKGWLKAVTRTFDWAEH"
     gene            3635..4792
                     /locus_tag="AC162G_07875"
     CDS             3635..4792
                     /locus_tag="AC162G_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5075767.1"
                     /translation="MSAPHVIVIGAGSTGAATAHDLALRGLRVTVIERGEVASGTTGR
                     NHCLLHSGGRYCVKDQESAIECIDENMILRRIMPDVLELNDGLFVAITEDDLAFKEKF
                     LAGCEACHIPARDLPLKRAFEVEPLLNPKTLAAVQVPDGVFEPFRFCLAFLATAHRNG
                     AIVRTYCEVKEVTRSGQSVTGVTVVDHRTGKTESIGADIVVNATGPWAGEIAEMAGVD
                     VPVSPTAGVMVTVGKRLNNMVVNRLNKPSDGDIVVPQRTTSIIGTTSWAVDDPDLIPI
                     PQEHVDKMFQQGEMLLPGIRQVPLRGVMAAARPLISKGGVDEREVSRTFECFDHKRDG
                     IDGFVTISGGKTTTARAMAEKVSDIVCNKLGIGAECRTRDTPLASYRLFYQ"
     gene            5042..5809
                     /locus_tag="AC162G_07880"
     CDS             5042..5809
                     /locus_tag="AC162G_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HIR5075768.1"
                     /translation="MILEVALKVFRYKAGQPPRYDTFRVQVADTANVLDAIEAAWAQH
                     DRSLTFRHACHHASCGSCAVRIDGCEKLPCITPVRDVWRGRGDLRIEPLRNFPIVSDL
                     VVDVSGFFQRMSTSGMVITRAAEAYLPVPAGAADGRPQDARSVTLPEGVARANRFENC
                     IECGICISACPTTATSDKFMGPAALAGIYRARLEASNKAESARLLELSDGEHGVWRCH
                     SGWECTEACPQGVDPAGAIMLLRRDLVKHRIRKLFGR"
     gene            5811..>6171
                     /locus_tag="AC162G_07885"
     CDS             5811..>6171
                     /locus_tag="AC162G_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013307.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     transmembrane subunit"
                     /protein_id="HIR5075769.1"
                     /translation="MATRLQSSRLRRTAQWADVRRRGMGMWAYTLNRVTGIGLVVYLY
                     LHLVVLSMLAGGPDMWDPFVNLARSPLFLALDVILIAGWLIHGLNGIRVTLTGLGFGT
                     RSQKSLFVGLMLIAGIGT"
BASE COUNT         1186 a         1914 c         2026 g         1045 t
ORIGIN      
        1 gcgacctcgg tccgcgtatt gcacaattcc tcctgccgat ccctggcaag cgcgacagcg
       61 attggggcta ctcgtggatc ccggtagtcg gtccggccat aggcggtgtg ctggccgcgt
      121 tcctggcgca ggcgctgggc atgtagcatc agcctcaacc attcactgac agggggcacc
      181 gatgaagaaa ctgatcaaca agcccgaaga cgtcgtcaag gaagagcttc agggcatcgc
      241 cgcggcccac cctgacctgg tcaaggtgtt cttcgagccg aactacatcg tgcgcgccga
      301 cgcgccggtg aagggcaaag tgggcgtcat ctccggcggt ggctccggcc acgagccgat
      361 gcacggcggc ttcgtcggcc ccggcatgct cgacgcggcc tgcccaggtg ccgtgttcac
      421 ctcgcccaca cccgaccaga tgctggaagc cacgaaggcc gtgaacgggg gagccggcgt
      481 gcttcacgtc gtcaagaact acaccggcga catcatgaac ttcgagatgg ccgccgacct
      541 ggcgcggggc gagggtatcg aggtggagag cgtcgtcacc aacgacgacg tggcggtgca
      601 ggacagcctg tacaccgctg gacggcgcgg cgtcggcgtc accgtgctgt cggagaagat
      661 ctccggcgcg gccgccgagc agaagcgctc gctcaaagaa gtggccgaca tctgccgcaa
      721 ggtcaacgcg aacggccgca gcatgggcat ggcgctgact tcatgcaccg tgccgcatgt
      781 cggcaagccg acgttcgatc tgccggagga cgagatggag atcggcattg gcatccacgg
      841 cgagccaggc cgcacgcgca tgaagctcaa gacggcggcc gagatcaccg agatgctcgc
      901 catacccgtg ctcgacgatc tgcccttcaa gtccggcgac gaggtgctgg ccttcgtcaa
      961 cggcatgggg ggcacgcccg tcatcgagct gtacgtggtg tataatgagc tggcgaagat
     1021 ctgcgccggt cgtagggtga agattgtgcg caacctcatc ggctcgtaca tcacctcgct
     1081 cgaaatggcc ggatgctcca tcaccctgct caagctggac gacaacctga ccaagctgtg
     1141 ggacgcgccg gtcaagacgg ccggcatgcg ctgggggatg taagatcgac gggtgaccga
     1201 tgacgaagga cgaaagattg gtcgttgttt gcgccatccg tctttcgtcc ttcgtcgcaa
     1261 ttcatggggg ccagagtgac caacagtgta tcgcgggacg ccgttctcgc atggatcagg
     1321 gcctacgcgg ccgtcatcgc cgacaacaaa gagtatctga ctcagttgga tgcggccatc
     1381 ggagacgccg atcacggcgc gaacatggat cgcggttttc aggctgcact caccaagctg
     1441 cccggcgtct ccgacaagga tatcggcgcg atcctgaaga cagtcggcat gacgctggtc
     1501 tctaccgtcg ggggggccgg tggcccgctc tacggcacgc tcttcatgca gatgggaact
     1561 tcgtccgcgg gcaaactgga gctcactctg gccgactggg tcgacgcgtt agaagccggc
     1621 gtgaacggtg tggtcatgcg cggcaaggcc aacctcgggg acaagacgat ggtcgacgcg
     1681 ctctcgcccg cgctggtgac gctgaaagag gcggcggcta atggcacgcc tatgcgtgag
     1741 gcgctcgtga agtcggccca agctgccgag cagggcatga tcgccaccat tcccttggtc
     1801 gccaagaagg gccgcgccag ctacctgggc gagcgcagcg ccgggcatca agacccgggc
     1861 gcgacctcgt cgcacttgct cctcaaggcc gctgccgata cctggactaa cggcggctag
     1921 cgtcggcgga atatgccctg ttggtacgac gcatggcggc ctaccgaaca ggcacaacgc
     1981 tctatcagat ggaggcataa gatggcgaaa tacgcagcag caatcgatca aggcacgacc
     2041 agcacccgct tcatgatctt cgatcacggc ggcaaggtcg tggaggtcga tcaacgtgag
     2101 cacgagcaaa tttacccaaa gcccggctgg gtcgagcacg acccgatgga gatctggcag
     2161 cgcacgcagg aggtggtcaa ggaggcgctg accaagagca agatcgatcc caaagacatc
     2221 tccgccatcg gcgtgaccaa ccagcgcgag acgaccatcg tctgggagaa ggcgacgggc
     2281 aagccggtct acaacgccat cgtctggcag gacacccgca cggccggcat ctgcaacgat
     2341 ctggcaaagg acgggggaca ggaccgcttt cgccccaaga ccggcctgcc gctggcaacc
     2401 tacttctccg gccctaagat caagtggatc ctcgacaacg tcgaaaacac gcgggccagg
     2461 gccgagaagg gcgaactgct cttcggcaac atcgacacct gggtgatctg gaacgtcacc
     2521 ggcggcccga agggtggcgc gcacgtcacc gatgtgtcca atgcgtctcg caccatgctg
     2581 atgaacctgg agacgctgga ctgggacgat gagatcctga agatcctgaa catcccgcgc
     2641 gctatgctgc caaaggtccg gccgtcgagc gaagtgtacg gtacggcagt gggtgacctg
     2701 gcgggcgttc ccgtcgccgg cgacctcggc gatcagcaag gtgcgctatt cggtcagacc
     2761 tgcttctctc ccggcgaggc caagaacacc tacggcacgg gctgcttcat gctgctcaat
     2821 accggcacca agcctgtgcc ctcgaagagt ggcctgctca cgacgctggg ctacaagatc
     2881 ggcgatcagc cggcggtgta ctgcctggaa ggctcgatcg cgatcaccgg cgcgctggtg
     2941 cagtggctgc gcgacaacct gggcatgatc cagaagtcgg ccgacgtcga ggcgctggcc
     3001 aagaccgtcg aagacaacgg cggcatctac ttcgtcccgg cgttctccgg cctgttcgcg
     3061 ccgtactggc gcaacgacgc gcggggcgtg atcgtcggca tgacccgcta cgtcaacaag
     3121 ggccacatcg cgcgcgccgc gctggaggcc acggccttcc agacgcgcga agtgctggac
     3181 gcgatgaata aagactccgg cgtccctctc aaagcgctca aggtcgatgg cggcatggtg
     3241 tacaacgagc tgctcatgca gttccaggcc gacctcctgg gcgtgccggt catccgccca
     3301 accgtggccg agaccaccgc actgggcgcg gcctacgccg ccggcctggc cgtaggtttc
     3361 tggaggaacg tcgaggacct gcgcgccaac tgggcgaaag acaaagagtg gcagcccaag
     3421 atggacgtgg agcaccgcga gaagctgcac aagggctggc tcaaggccgt cacgcgcacg
     3481 ttcgactggg ctgagcacta aggattgctg aagacttcgg aagtcttcaa gagacttccg
     3541 aagtctggag ctggtacgtc actggcggaa gcctggaagc cgggttgcag gcttccaggc
     3601 tttcttgcat atcggtacga gagcaagaga gtctatgtct gcccctcacg tgattgtcat
     3661 cggcgccggt tcgaccggag cagcgaccgc gcacgacctg gcgctgcgcg gcttgcgcgt
     3721 caccgtgatc gagcgcggcg aagtcgcgtc cggcacgacc ggccggaatc actgcctgct
     3781 gcactccggt gggcgttact gtgtcaagga tcaggaatcg gcgatcgagt gcatcgacga
     3841 aaatatgatt ctgcgcagga tcatgccgga tgtattggag ctgaacgacg gcctgttcgt
     3901 ggccatcacg gaagacgatc tggccttcaa ggagaagttc ctggcgggct gcgaagcgtg
     3961 ccacatcccg gcgcgcgacc tgccgctcaa gcgcgcgttc gaggtcgagc cgctcctcaa
     4021 tccgaagacg ctcgccgccg tgcaggtgcc cgacggcgtg ttcgagccgt ttcgcttctg
     4081 cctggcgttt ctcgccaccg cgcaccgaaa cggggccatc gtgcgcacgt actgtgaagt
     4141 caaggaagtc acgcgctccg gccagagcgt gaccggtgtg accgtcgtgg atcaccgcac
     4201 aggcaagacg gaaagcatcg gggccgacat cgtcgtcaac gcgaccggac cgtgggccgg
     4261 cgaaatcgcg gagatggccg gtgtcgacgt gccggtatcg cccaccgccg gtgtgatggt
     4321 caccgtcggc aagcggctga acaacatggt cgtcaaccgc ctgaacaagc cttcggacgg
     4381 cgacatcgtc gtcccccagc ggacgacgtc gatcatcggc acgacttcgt gggctgtcga
     4441 cgaccccgat ctgattccga ttccgcagga gcacgtcgac aagatgttcc agcagggcga
     4501 gatgctgctg cccggcatcc ggcaggtgcc gctgcgcggc gtgatggccg cggcgcgtcc
     4561 cctcatcagc aaaggcggcg tcgacgagcg cgaagtcagc cggacgttcg aatgtttcga
     4621 ccacaagcgc gacggcatag atggcttcgt cacgatttcc ggcggcaaga cgacgacggc
     4681 ccgcgccatg gccgagaagg tctccgatat tgtatgcaac aagctaggga tcggagccga
     4741 gtgccgcacg cgcgatacgc cgctggcgtc ctatcggctt ttctatcagt gattggagtt
     4801 tggcaacggc tatctctggt gtctggagtg caggcttttg cagcttaaca aatagatccg
     4861 acaatccggg ggcaggttct tctatgcctg ccttgaaggc ggctatcgaa agccgcccta
     4921 cgtcgcctgc gtttagtgtc ggattaaatt gttaacattc aacagcctgc ggaggccgaa
     4981 gatatggcgg cctgatggcc gcactccggc aaattgccgg agtccagtca gtgagatcga
     5041 tgtgatactc gaagtcgcgc tcaaggtctt ccggtataag gccggccagc caccgcgata
     5101 cgacacgttc cgcgtccagg ttgcagatac ggccaacgtg ctcgacgcca tcgaagcggc
     5161 atgggcgcag cacgaccgca gccttacctt tcggcatgcc tgccaccacg cctcgtgcgg
     5221 atcgtgcgcc gtgcgcatcg acggctgcga gaagttgccg tgcatcacgc cggttcgcga
     5281 cgtgtggcgc ggccgcggcg acttgcgcat cgagccgctg cgcaactttc ccatcgtcag
     5341 cgacctcgtc gtcgacgtaa gcggcttctt tcagcgaatg tcgacgtcgg gcatggtcat
     5401 cacgcgcgcg gcggaagcgt atctgcctgt gccggccggc gccgcagacg gccggccgca
     5461 agacgcgcga tccgttacgc tgcccgaggg cgtggcgcgc gcgaaccgct tcgagaattg
     5521 catcgaatgt ggaatatgta tcagcgcctg cccgacgacg gcgacgagcg ataagtttat
     5581 ggggccggcg gcgctggccg ggatttaccg cgcccgcctc gaagcgtcga acaaagcgga
     5641 atcggcgcgg ctgctcgagc tgtccgatgg tgagcacgga gtgtggcgct gccacagcgg
     5701 ctgggagtgc accgaagcct gcccgcaagg ggtcgacccg gccggggcca tcatgttgct
     5761 gcgccgcgac ctggtcaagc atcgaatcag gaaactattc ggacggtgat atggcgacac
     5821 gacttcaaag ctccaggctg cgccgcaccg cgcaatgggc cgacgtgcgc cggcgcggga
     5881 tgggaatgtg ggcttacaca ctcaatcgcg tgaccggcat cgggctggtg gtctatctct
     5941 acctgcactt ggtcgtgcta agcatgctgg ccggcgggcc ggacatgtgg gatcccttcg
     6001 tcaacctggc gcggtcgccg ctgttcctgg cgctcgacgt cattctcatc gccgggtggc
     6061 tgattcacgg gttgaacggc attcgcgtga cgcttaccgg gctgggcttc ggcacgcgca
     6121 gccagaagag cctgttcgtc gggttgatgc tgatcgcggg gatcggtacc g
//
LOCUS       DBNHXB010000195         7965 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3231420, whole genome shotgun sequence.
ACCESSION   DBNHXB010000195 DBNHXB010000000
VERSION     DBNHXB010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7965)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3231420"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..438
                     /locus_tag="AC162G_07890"
     CDS             22..438
                     /locus_tag="AC162G_07890"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HIR5075770.1"
                     /translation="MNTKRSDITPELEALFPRIYEVVKQVPWGTVTTYGNVAQIVGAG
                     CDARLVGYAMAGVNDPDVPWQRVINAQGKISPRAGRGAELQRKRLEAEGVEFDERGRV
                     DFERFGWRGPDPDWARQHGYHTLKPKEEKPNQASLF"
     gene            490..1533
                     /locus_tag="AC162G_07895"
     CDS             490..1533
                     /locus_tag="AC162G_07895"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HIR5075771.1"
                     /translation="MRTYEIAGNLHVHTPFSDGEMYHADIAQAAVAAGLDFVVVTDHN
                     VWVKGPEGYHDGVLVLVGEEVHDNRRQPQANHLLIYGADAEMCLHAESPQGLIDAARA
                     RGALTFLAHPVDPPLRFIGEEALDWVDWDVEGYTGIEVWNYMSEFKGRIPNRVMALWN
                     VFNPQRTIRGPFRQILAIWDSLLAQGRRVTAIGGADAHGATYSLGLISRTVFPYEYLF
                     RCVNTHLLIEQPLSGDVATDKQSIYAALAHGRGWIGYDLLGSTKGFRFMARSMANSAG
                     IGDDLKRAAAVTFEVGTPAPGRIRLICADKGVVAASGGRTLKYTTADAGAYRVEVTRG
                     GKGWIFSNPIYVR"
     gene            1781..2485
                     /locus_tag="AC162G_07900"
     CDS             1781..2485
                     /locus_tag="AC162G_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075772.1"
                     /translation="MPQPTRNPPAPTSTGRAFPTFPARHTRTPWPTAVPAPSTTPASA
                     PPTMPVGPPTAAATASANGAPAGQPAATRISHMPALATIQSTLLSQGINSNVYWFDSG
                     GESQLMIQYDSPIRNRPGYADMLEKVKQVAAQHFLRIDPPLYTMWIAATDMTGTSDTV
                     LRLDRYTVERWTRGEIGDADFYNNGFVPARIVMTCDASGCAGVRPTPYPTFPSFPFPF
                     PTPTGIPTIQLPTPTP"
     gene            2683..2868
                     /locus_tag="AC162G_07905"
     CDS             2683..2868
                     /locus_tag="AC162G_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoisovalerate dehydrogenase"
                     /protein_id="HIR5075773.1"
                     /translation="MLKEIIFLVKEAPQGGFTACASGYSIFTEADTWEELRDAVCDAV
                     ACHFEDDQQPTFLHPPS"
     gene            complement(3082..3441)
                     /locus_tag="AC162G_07910"
     CDS             complement(3082..3441)
                     /locus_tag="AC162G_07910"
                     /inference="COORDINATES: protein motif:HMM:NF039496.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HIR5075774.1"
                     /translation="MMDIDAFFQGFAQSRSIFETLRQSVEGLQPTTVSVTKSQIAFRQ
                     RRAFAWAWVPDRYLRGRHAPLVLSVALRRRDHSQRWKQVVEPTPGRFMHHLELNSPAD
                     IDDEVLQRLREAWDEAG"
     gene            complement(3590..4471)
                     /locus_tag="AC162G_07915"
     CDS             complement(3590..4471)
                     /locus_tag="AC162G_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075775.1"
                     /translation="MHTEISDIRKHIIERILVKQDDSFRWGFDEKQARERPEYLYYSP
                     NYKSTLWTLLLLADIKAPVDIPQIEPSIRLITERFYDPEHRIFRLLGMSHFPIPCLNG
                     NMIYLHRYFETSHSETLDRTIGFFAAYQRFDDGGFKTPKTYPYCSNTSCYGKHTCYWG
                     VTKLLKGVSFIPKNQRTQEAQRLIENCIDFVLHHEVCFRSHNRDRFLHRDIGKLTFPN
                     FYKSDFLEILWLLAREEVHDRKMSRALELLRSKMKDGGFWELEKSMNIIVSIGQKDCA
                     NAFITERATEVLDYYGR"
     gene            complement(4828..5262)
                     /locus_tag="AC162G_07920"
     CDS             complement(4828..5262)
                     /locus_tag="AC162G_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075776.1"
                     /translation="MIEELTDTLQAIALLGQVFDSSVFVGTIYSRGEALGEYIDYPEY
                     PLEFENVSLLYLNRDVSIEQRATEWASLFGVPQHSSALADVFRRRDEYHFAEDFYTSL
                     LEALALPTVMVGATYWELVRAYETDPEAQKALIHASGVSPES"
     gene            5833..7893
                     /gene="uvrB"
                     /locus_tag="AC162G_07925"
     CDS             5833..7893
                     /gene="uvrB"
                     /locus_tag="AC162G_07925"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR5075777.1"
                     /translation="MSQKFELVTDLRPTGDQPEAIVKLVDGIKKGFKHQTLLGATGTG
                     KTFAIAHVIQQTQKPTLVMAHNKTLAAQLYAEFREFFPKNAVEYFVSYYDYYQPEAYI
                     AKTDTYIEKDTSINEEIDRLRLSATSAVMSRRDVIVVASVSCIYGIGNPEDWGKVVIH
                     VATGETRRRDLLLRHLVDVQYERNDVNFARGRFRVRGDTLEVFPAYAETAYRVEFWGD
                     AVERITEIDTLTGEILKAEASVSIYPAKQFITVEDKLKRAIEKIERELAERVQWLKQN
                     DLLLEAQRIEQRTKYDLEMLREVGYCAGIENYSRHLDGRAPGEPPWTLVDYFPDDYLL
                     MMDESHISVPQVRGMYHGDRSRKEELVKYGFRLPSALDNRPLTFEEFEARMGQTIFTS
                     ATPAEYELKRSQQVVQQIIRPTGLVDPQIVVRPVKGQVDDLVGEIQKRVARGERVLVT
                     TLTKRMAEDLADYLKELNTKVHYLHSEIQTIERTEILRDLRLGHYDVVVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSTTSLIQTMGRAARHVHGTVIMYADTVTESMQRAIDET
                     NRRRDIQARYNEAHNIVPASIVKAVRDLTDSIRVAAEEKADYRVSRKIPKDELARLIK
                     ELEKQMKQAAHDLEFEKAALLRDQIFELRQQMEEADEKKKPAWQRLRDLPAEVVADEL
                     VGEN"
BASE COUNT         1694 a         2405 c         2335 g         1531 t
ORIGIN      
        1 gcactgagca ggcgctgtgg gatgaatacc aaacgaagtg acatcacgcc agaactcgag
       61 gcgctcttcc cgcgcatcta cgaggtcgtc aagcaagtgc catggggcac cgtcacgacc
      121 tacggtaatg tggctcagat tgtcggcgcg ggctgcgacg cgcgcctggt cggctatgcg
      181 atggccggcg tgaacgaccc cgacgtgccc tggcagcgtg tgatcaacgc acagggcaaa
      241 atcagcccgc gcgccgggcg cggagcggaa ctccagcgca agcgactcga ggccgagggc
      301 gtcgagttcg acgagcgtgg gcgcgtcgac ttcgagcgct tcggctggcg cggtccggat
      361 cccgattggg ccaggcagca cggctatcac acgctcaagc cgaaggaaga gaagccgaat
      421 caggcaagtc tcttctaagg tgaccggcac tttgaaagtg ccggtcacct tcaaagttcg
      481 ttgacacgca tgcgcactta cgaaatcgcc ggcaacctcc acgtgcatac acccttctca
      541 gacggcgaga tgtaccacgc cgatatcgcc caggctgccg ttgccgcagg tctcgacttc
      601 gtcgtcgtca ccgaccacaa cgtctgggtg aagggaccgg agggctatca cgacggagtg
      661 ctcgtcctcg tcggcgagga ggtgcacgac aatcggcggc agccacaggc caaccacctg
      721 ctcatctacg gcgcggacgc cgagatgtgc ctgcacgccg aatctccgca ggggctgatc
      781 gacgctgcgc gcgcgcgcgg cgcgctcacc ttcctggctc atccggtcga tccaccgctg
      841 cgcttcatcg gcgaagaggc gctggactgg gtcgactggg acgtcgaggg ttacaccggc
      901 atcgaggtgt ggaactatat gtcggagttc aagggtcgca tccctaatcg cgtgatggca
      961 ttgtggaatg ttttcaaccc gcagcgcacg attcgaggcc cgtttcggca gatactcgcc
     1021 atctgggata gtctgctggc ccagggtcgt cgtgtgacag ccatcggcgg cgcagacgcg
     1081 cacggcgcga cttactcact cggccttatt tcgcgcacgg tgttccccta cgagtatctc
     1141 ttccgctgcg tcaacacgca cctgctgatc gagcagccgc tcagcggcga cgtggcgacc
     1201 gacaagcagt cgatctacgc ggcgctggcg cacgggcgcg gctggatcgg atacgacctg
     1261 ctcggcagca cgaagggctt tcgcttcatg gcgcgcagca tggcgaactc agcgggcatc
     1321 ggcgacgacc tcaagcgcgc ggctgctgtc accttcgagg tgggaacccc ggctcctggc
     1381 cgcattcgac tgatttgcgc tgacaagggc gtcgtcgccg cgagcggcgg acggacactg
     1441 aaatacacga cggccgacgc aggagcttac cgggtggaag tcactcgggg cggcaagggc
     1501 tggatcttca gcaatccgat ctacgtgcgc tgatggcgaa tcgcttatcg cagatagctt
     1561 atcgctgatg gacgatggtt tttcgggcaa ggcgcacaac ggaccagcca actaaccaac
     1621 tatcctgtgc tcgcggcccc ttcgggtgat ccatccgccg ccttcagccg ccggatggat
     1681 catccagcgg gagcgggtgc caaacatggg ctgagatcca gctattatgc gtttgcttct
     1741 actcttgtcg acagtagcac tcgccttcgc aggctgcgcg atgccgcagc cgacacggaa
     1801 tccgcccgct ccaacttcaa ccggccgcgc gttccccacg tttccagcac gccacacacg
     1861 cacgccctgg ccgacggcag taccggctcc cagcacgacg ccggcctcgg cgccgccaac
     1921 aatgccggtt ggccctccca cggcagcggc gacggcaagc gcaaacggcg cacctgccgg
     1981 ccagcccgcg gcaacccgca tcagccacat gccagctctt gccactattc aatccacgct
     2041 cctgtctcag ggcatcaact cgaatgtcta ctggttcgat tccggcggcg agtcgcagtt
     2101 aatgatccag tacgacagcc cgatccgcaa ccggcccggc tacgccgaca tgctggagaa
     2161 ggtcaagcaa gtcgccgcgc agcactttct ccggatcgat ccaccgctgt acacgatgtg
     2221 gattgcagcg accgacatga cgggcacgag cgacaccgtt ctgcgcctgg atcgctacac
     2281 cgtcgagcgc tggacgcgcg gcgagatcgg cgacgccgac ttctacaaca acggctttgt
     2341 accggcacgc atcgtcatga cctgcgatgc cagcggctgc gcaggcgtca gaccgacgcc
     2401 ctacccgacc tttccgtcgt tccccttccc gttcccgaca ccgactggaa ttccgacgat
     2461 ccaactgccg acgcctacgc cctgagcaag gagtcagcag ccccagatgc ggcctccagg
     2521 gcggaatctc aagtgcaaac gcatccttca acgcctgccc gtgaaccgat gcgagcctcc
     2581 gctcagaatg cttacccaat tttggatctt gaccggcaca cgggggatgc cgcggttgtg
     2641 caggtcaagg tataatctcc gcagcagcct gagggaccgc acatgctcaa ggagatcatt
     2701 tttctcgtca aagaagcgcc gcagggagga ttcacggcgt gcgcatcggg atactctatc
     2761 ttcacagagg ccgatacttg ggaagagctg cgtgatgcgg tatgcgacgc cgttgcgtgc
     2821 cacttcgaag atgaccagca gccaaccttt ctccatcccc cgtcatagcc cctttaggat
     2881 aggaacgctc aactcaattc tgaaagatat ggctcagcac ttcgggatgg gccgagaagc
     2941 tctgatcaat tcgttgctcg atcggtaaac agcccatttt ctcccgctca tcctccgcag
     3001 tgtgcagctt cgagcgaacg gggtgcactc tggtcgttat cacggccgga cttgctccgg
     3061 tgacgcatgg atggggtttg cctatccggc ctcgtcccag gcctcccgta gtctctggag
     3121 gacttcgtcg tcaatgtcgg ccggagagtt gagctccagg tggtgcatga accggccagg
     3181 tgtcggctcc acgacctgct tccaccgctg cgaatggtcg cggcgcctga gtgcgacgga
     3241 gagaacgagt ggggcgtgtc tgccgcggag gtaccggtct ggcacccatg cccaagcaaa
     3301 ggctcttcgc tggcggaagg cgatctggct cttggtcacg gagacagttg tcggttggag
     3361 gccttcgacc gactgtcgca aggtctcgaa gatagacctg gactgggcaa atccctggaa
     3421 gaaggcatcg atgtccatca tggacgattg agtcgcagcc ggcgcgattt gtcgcctcgc
     3481 cgaaggatcc tgcaggacgc ctaacggccg gcttcacctg cgccgcgaag cgcagcggag
     3541 cggcgtcagg tgcaagccgt tgttgggcgc atatggagat ttccctagat caacgcccat
     3601 aataatcaag cacctcggtt gctctttccg ttatgaaggc attggcgcaa tccttctgac
     3661 cgattgagac tatgatgttc atggatttct ccagttccca gaaaccgccg tccttcattt
     3721 tggatcgcaa caactccaag gcgcgagaca tcttcctgtc gtgcacttct tctcttgcca
     3781 atagccacag tatttcgagg aaatcactct tatagaaatt gggaaatgta agtttgccaa
     3841 tatcacggtg aaggaatctg tctctattat gggacctgaa acacacttcg tggtgtagaa
     3901 caaaatcaat gcagttttcg attaatcgtt gtgcttcttg tgtcctttgg ttcttgggaa
     3961 tgaacgaaac tcctttgagc agttttgtca ctccccagta acaggtatgc tttccgtagc
     4021 aggaagtatt cgaacagtac ggataggttt tcggggtctt gaaaccaccg tcgtcaaagc
     4081 gctggtatgc ggcgaagaaa ccaatggttc tatccagtgt ttctgaatga gatgtctcga
     4141 aatagcggtg gagatagatc atgttgccat tcaggcaggg gatgggaaaa tgactcatgc
     4201 ccagcagcct gaaaatcctg tgttcaggat catagaagcg ctcagtaata agccggatgg
     4261 atggttcgat ctgcggaata tcgacaggcg ctttgatgtc agcgagcagg agcaaggtcc
     4321 ataacgtgga tttgtaattg ggcgaatagt acaaatactc cgggcgctca cgggcttgtt
     4381 tctcatcgaa tccccaccgg aatgaatcgt cctgtttgac aaggattctt tcgatgatgt
     4441 gctttcggat atctgaaatc tctgtatgca tagactggtt ctctatttcg cctcaatgat
     4501 gcgcccaacg gacggagttc gcctgccgcc gggtggggca gcgatagact gccgaaagga
     4561 taccccgcag gcccagaacc ttcaagtgcc ggcgcggtag acggttacaa tgagttagat
     4621 ttattatacg cccaactgac acctgccgag gctatccatg aacaacacga tggctctgca
     4681 acagtttgaa cactatctgc gctggcgtcg ctcccagaat gcgcgctgac catctctgcc
     4741 gttgtaacga aagacacact tgacaactca ttgtaaagac ggtcggctgc atgcgcaggt
     4801 tgggcagcgg tttggaaccc ggccgcttca actctctggc gatacaccgc tcgcgtgtat
     4861 tagcgccttt tgcgcctctg ggtcagtctc gtaggctcgc actagctccc aataagtggc
     4921 cccgaccata acagttggaa gcgccagggc ttctaacaac gaggtgtaaa agtcttcggc
     4981 aaagtgatac tcatcacgcc tgcggaatac gtcggccagg gcgctactat gctgaggtac
     5041 gccgaacaga cttgcccact cggtcgctct ttgctcaatg gatacatccc ggttcaggta
     5101 cagtaacgac acgttttcga actcaagcgg gtattccggg taatcaatgt actcacctaa
     5161 ggcctccccc cgactgtaga ttgtgccgac aaacacgctg ctatcaaaaa cctgtcccag
     5221 cagggctatg gcttgcagtg tgtcggtcag ctcctcgatc acatcgaggt cagcttcgtc
     5281 atcattatca tcacaaatgg cgcatcggtt acggtcatct tgaaagatga aagcgcgtgt
     5341 gccttgcgct tgatagaaag cggctagtgc ttggcccgtg gaaccgacaa ctacgtagct
     5401 gttagtaagt agtcgacctt atgaacggtc gtgacgcctc gattgggctc gacggcacgc
     5461 tgcccagctc ttgattctac acaccaactg cgctgcaatt gattatgaca cactatagta
     5521 caccagcccg ctcgacacct gatgccgggc atggctaccc gcgttgccgg cagtatagga
     5581 gcgattcgtc tcgaagtcaa tcggcggtga taaaaacggg ggatgcaacg actggcccgc
     5641 ttcgggtata atcattaggt tgacaacgcc cattcgccga ctcgctcatt tagagactgt
     5701 cttaaaacct accaagaaga caccaagaaa aaccttgaaa tccttcgtgt cccttcggct
     5761 ccgctcaggg caggcttggt gacttcgtgg ttaacaaagg ccattttaag acgcgttctg
     5821 agaaaggcac ctatgtccca gaagttcgag cttgtcacgg atctcaggcc gacgggcgat
     5881 cagcccgaag cgattgtcaa actggtagac ggcatcaaga agggcttcaa gcaccagaca
     5941 ctgcttggcg cgaccggcac cggcaagacg ttcgccatcg cgcacgtcat ccagcagacg
     6001 caaaagccga cactggttat ggcgcacaac aagacgctgg ccgcgcagtt gtacgccgag
     6061 ttccgggagt tcttccccaa gaacgccgtc gagtacttcg tatcgtatta cgactactac
     6121 cagcccgaag cctacatcgc caagaccgat acgtacatcg agaaagacac gtcgatcaac
     6181 gaggagatcg accggctgcg gctgtcggcg acgtcggcgg tgatgtcccg gcgcgacgtg
     6241 atcgtcgtcg cctcggtgtc gtgcatctac ggcatcggca acccggagga ttgggggaag
     6301 gtcgtcatcc acgtggcgac gggcgaaacg cgccgccgcg acctgctgct gcgtcacctc
     6361 gtcgacgtgc agtacgagcg caacgacgtg aacttcgcgc gcgggcgctt ccgcgtgcga
     6421 ggcgacacgc tcgaggtctt ccccgcttac gctgagactg cctaccgcgt cgagttctgg
     6481 ggcgacgccg tcgagcgcat caccgagatc gacacgctca ccggcgagat cctcaaagcc
     6541 gaagcgtccg tgtcgatcta cccggcgaag cagttcatca ctgtcgagga taaactgaaa
     6601 cgcgcgatcg agaagatcga gcgggagctg gccgagcgcg tgcagtggct caagcagaac
     6661 gatctgctgc tcgaagcgca gcgcatcgag cagcgcacga agtacgatct ggagatgctg
     6721 cgcgaggtcg gctactgcgc tggcatcgag aactactcgc gccacctcga cgggcgcgcg
     6781 cccggcgagc cgccgtggac gctggtcgat tacttcccgg acgattacct gctgatgatg
     6841 gacgagagtc acatcagcgt cccacaggta cgcggcatgt atcacggcga ccgcagccgc
     6901 aaggaggagc tggtcaagta cggcttccgt ctgcccagcg cgctcgacaa ccgcccgctc
     6961 acgttcgagg agttcgaggc gcgcatgggg cagacgatct tcacctcggc cacacccgcc
     7021 gagtacgagc tgaagcgcag ccagcaggtc gtgcagcaga tcatccgccc gaccggcctg
     7081 gttgatccgc agatcgtcgt cagaccggtg aaaggccagg tggacgatct cgtcggcgag
     7141 attcagaagc gcgtcgctcg cggcgagcgc gtcctcgtca cgacgctgac caagcgcatg
     7201 gccgaggacc tggcggacta cttgaaggaa ctcaacacca aagtccacta cctccattcc
     7261 gagatccaga ccatcgagcg caccgagatc ctgcgcgacc tgcgcctggg acactacgac
     7321 gtcgttgtcg ggatcaacct gctgcgcgaa ggactcgacc tgcccgaagt gtcgctcgtc
     7381 gccatcctcg acgccgacaa agagggcttc ttacgcagca cgacctcgct cattcagacg
     7441 atgggccgcg ccgcccggca cgtgcacggc acggtgatca tgtacgccga cacggtcacc
     7501 gagtcgatgc agcgcgccat cgacgagacc aatcgccgcc gggacatcca ggcgcgctat
     7561 aacgaggcgc acaacatcgt cccggcgagc atcgtcaagg ccgtgcgcga cctgaccgac
     7621 tcgatccgcg ttgcggccga ggaaaaggcc gactatcgcg tcagcagaaa gataccgaag
     7681 gacgagttgg cgcggctgat caaggaactt gagaaacaga tgaagcaagc ggcgcacgat
     7741 ctggagttcg agaaggccgc gctgctgcgc gatcagatct tcgagctgcg ccagcagatg
     7801 gaagaagcgg acgagaagaa gaagccggcg tggcagcgcc tgcgcgactt gcccgccgag
     7861 gtcgtggcgg atgagctggt tggtgagaat tgaggagtgc gacatgaccg aaccgggcag
     7921 gcgactacgc cggcgcgctc gaatgggtcg aatcccacgg tggtg
//
LOCUS       DBNHXB010000196        12779 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4573506, whole genome shotgun sequence.
ACCESSION   DBNHXB010000196 DBNHXB010000000
VERSION     DBNHXB010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 12779)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12779)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12779
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4573506"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="AC162G_07930"
     CDS             <1..582
                     /locus_tag="AC162G_07930"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5075778.1"
                     /translation="SMPALETERLIIRPFAMDDLDAIHRILDVELAEVDFGSEEAKTL
                     DARREWLRWTILNYDQLARLYQPPYGERAIVLKSSDELIGAAGYVPCLNVFGQLPVSG
                     GTRDGLTSTEFGLYYALSPAHHRRGYAAEAVRAMIDYAFRRLRLRRIIATTTYNNKAS
                     MGVMRKVGMRIEKNPFPDPPWLQVVGVLEAPST"
     gene            631..1788
                     /gene="rsgA"
                     /locus_tag="AC162G_07935"
     CDS             631..1788
                     /gene="rsgA"
                     /locus_tag="AC162G_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR5075779.1"
                     /translation="MEGTLTGKRRARRKVIRHNTAVNQEKHRADWLPLAPNELGVVAD
                     RRIMPRDERDRRRALEEASAQSIDAAIGEADWLAAAQASGHRLGVVLEIAKDLCRVSV
                     DGRAVLCDIRGTLTAEGTGFTNIVAVGDRVLISLPEADHGWVEQVLQRRSALARPDSF
                     YSHLKQIIAANMDQLLIVASWREPHIWRELIDEYLIAAARNNLEAFICINKIDLAEDI
                     AECRAAVQAYPDLGYHLLLTSAKTGAGVDDLRQILRGKSTVLAGLSGVGKSSLLSAVQ
                     PGLKLRTRAVSEYSGEGRHTTKQVTMLPLREGGYVVDTPGIREMGLRGLYRDELVEFY
                     PEILAASPNCRYSDCSHIGEQVCGVKSAVAAGRIADWRYDNYRKLCAKLPE"
     gene            1929..3254
                     /locus_tag="AC162G_07940"
     CDS             1929..3254
                     /locus_tag="AC162G_07940"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR5075780.1"
                     /translation="MTKTLIKNVHVITLDETGAVLRNASIAIDGRTILAVGDVPSDFA
                     PDETIDGYNHVAVPAFFNAHCHAPMTYERGWAEDLPFPRWLNEKIWVAESALTPDDVY
                     WGAALAACEMIRSGVAAFNDHYFYMDRVAEVVEQSGLKAGLAWCVFGIGDDKEIGADL
                     DGTLAFVERWQDRAGGRLRLMLGPHSPYVCPPDFLARVAAIAHERGLPIHLHAAESQG
                     QVDNSMHAHGKSPVAHLAALGVLDGPCIVAHCLVVSDEDLDILAWHGVSVAHTPITYM
                     KLAMGVNDLSRFFAHGVNVAIGTDGPASNADMDMLAAIRQTAILQKHEQRNPETLPGD
                     TALRMASQNGARAMGFPDSGAIAPGRAADITLIDTDRPHLLPRHDLVANVVHSAKAAD
                     VTHLFVDGRLLYRNGEILTLDEDKIKTEAERGAFRMVGSEMRRVREYLG"
     gene            complement(3495..6710)
                     /locus_tag="AC162G_07945"
     CDS             complement(3495..6710)
                     /locus_tag="AC162G_07945"
                     /inference="COORDINATES: protein motif:HMM:NF019311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HIR5075781.1"
                     /translation="MNIHNFFNRFLLIMALFAAAAIVSACGAQRTPEPTIAPSVEPTI
                     TPTAPPATPAVIALNPASGGPGTPVTIVGVGFPASAHVAIRFGPPNAGAALQPFGDAI
                     ADANGAINIVVFVPGAWADGTPITGDRLVVVAATDDFVSKATADFAFQPPVTEPTPGA
                     TVSPTPPGAACSNRATFVSDLTIPDKTVVAPGASFIKAWRLRNSGTCAWDASYALVFA
                     GGDAMGGPATWPLPGSVPPGATVDVSVSLVAPAAAGTYRGNWLLRDGKRVLFGIGDDG
                     QQPFWVQVVVGPTPTPAVIGWRAEYYANRSLSGAPALVRGDPDVNFNWGAFSPAASIP
                     ADDFSARWTRSLGFAAGVYRFYVRSDDGVRVWLDGELIVDQWHDATNVTYSADRALSA
                     GLHAVRVEYYENRGDAQIQFWWERPGEFPEWRGEYYANASLTGAAKLVRNDGSISFNW
                     GGGAPDASLPADNFSARWTRTMGFDEGTYRFRALVDDGVRVYVDGALIVDEWREGGVR
                     EVAADVLLAAGNHALRVEYFERVGSALLQVWWEKFSTYPDWKGEYWSNRTLSGGPVVT
                     RNDKTVDFNWGAGTPAQVVPADNFSARWTRSLNFEAATYRFHILVDDGTRLWVDGGLV
                     IDDWRDGAAREITADVTMAAGFHGLKVEFYERTGDARISLWWEKLTPSFTDWKGEYWS
                     NRNLSGNPALTRNDKAIDFNWQGGSPAPGLPVDGFSARWSRQLSFEPGVYRFVAISDD
                     GVRITVDGRLVLNEWHDSVGDTPYTADVSLGGAHQIVVEYYENGGLARISVVWQRLPT
                     PTATAFPTRTPTATATSTPTTAPVTPSVTPTASATPTTVPVTPSATPTVSATPTATAT
                     QAPPSVTPTATATQAPPSVTPTATATQAPPSVTPTATATQAPPSVTPTATATQTRTPP
                     TLTGVRLNEILPAPGAVDWDGSGTANPQDEWIELTNTGVITVEIGGWSIESATAGAAY
                     EFPAGTVLGPGKFLVLYRQMTHIPLNDNGDGVRLLRPGGQVLELVIFGALGADRSFSR
                     DEYGVWHDDWSPSPGAPNVPPGGIASVPLRVVEPKGRPKVR"
     gene            complement(6938..7636)
                     /locus_tag="AC162G_07950"
     CDS             complement(6938..7636)
                     /locus_tag="AC162G_07950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5075782.1"
                     /translation="MPDYEWIYRQRAAEYDALVTREDYQRNILRTLRQIRPLHGLDVV
                     EMGAGTGRLTRLIAPHARSIAAFDASKAMLDVAAGKLPYLPARNWLLAVADNCCLPAR
                     DGIADICVSGWSFGHATGWYPDSWRKVVRLAVAQMIRVLRPGGTAIILETLGTGREMP
                     EPPTDALAEYYRLLEEEHDFRSRWIRTDYRFESVEEAERLARFFFGDALADRVARERL
                     AILPESTGVWYRVV"
     gene            complement(7647..8129)
                     /locus_tag="AC162G_07955"
     CDS             complement(7647..8129)
                     /locus_tag="AC162G_07955"
                     /inference="COORDINATES: protein motif:HMM:NF015669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HIR5075783.1"
                     /translation="MKLSNLMFLLAIILIASGCAPSGSVPAPTAEPTVPPSATDALAN
                     TNWRLVSFGVAGAESPVIEGTSLTLEFGADGQVGGSGGCNSFGGEYQVNGTALSISNI
                     VSTLIACADERVMEQEQRYFQALEASARFELAGDRLTISHDDGQGALNFVKDSPSALW
                     "
     gene            8454..9068
                     /locus_tag="AC162G_07960"
     CDS             8454..9068
                     /locus_tag="AC162G_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075784.1"
                     /translation="MRNRLSRIVLSLVVGSIVVAIGLYLGWFVAVGLQAARTLSGSVR
                     DQPSRETVALVTDVTRWTFLGLTTGLGLAFAALHKRRLRFLLASIAGFALGGALASWL
                     VVTSLDRAAPAATLALPLGGALAGLLLGLGARLDIRSALMLIVVALAMMIAGPFIDPR
                     SSVVLPSPLRLTPGDWLILLAPGALIGAILAALVSNGSDTSQTA"
     gene            complement(9109..9339)
                     /locus_tag="AC162G_07965"
     CDS             complement(9109..9339)
                     /locus_tag="AC162G_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075785.1"
                     /translation="MRDVVFISSASYRPAGGAPARLDTLLASAEAALARAEEHLRRAH
                     DARVHGVLPRVFSRAALDRYPSAADDPQSPVH"
     gene            10101..11120
                     /locus_tag="AC162G_07970"
     CDS             10101..11120
                     /locus_tag="AC162G_07970"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010036888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5075786.1"
                     /translation="MNESLAIETEKLGRIYKIRGAKKGEARELVALQDVDLEVRRGEL
                     FGLLGPNGAGKTTLIKILTTLLLPSSGRALVDGLDVVTQHRLVRERINMVSGGESSGY
                     GLLTVRENLWMFAQFYGIDHATTYRRIDEMLEVVGLSDRKNTKISDLSTGLRQKMNIV
                     RGFITDPQILFLDEPTLGLDVAAARDARQFVRRWIGRRDKTILLTTHYMVEADELCDR
                     VAIIDRGRVLACDTPASLKRRLQRDALFHIETSYLSTGGADGFEAIPGVSKVTHRHRD
                     DRTAFEFLLQGEDVLAPVVARMTEQNAKILSLQKREPTLEDVFIDLVGRGLTDGENGN
                     GSGNH"
     gene            11101..11958
                     /locus_tag="AC162G_07975"
     CDS             11101..11958
                     /locus_tag="AC162G_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5075787.1"
                     /translation="MEAAITNAPTRPPFKRRANLFWRTVFARSYPRLIGLRREPSWMF
                     FEIALPFLIACSYVFVYKAIGAPQEFIGFVVLGGAMSAFWLNMLWMMASQLFWEKQSG
                     NLPLYIAAPNSMTAILLGMALGGSAATCVRAAVIVVTGSVLFGVTYSTVNLLPALAVF
                     LLTMVALYGLGMLFASVFLLFGREAWHTSNLLQEPVYLVSGFYFPIKSLPVWVAAVAS
                     LVPVTLGLDAMRQLLFAGGETIGFLPVSIEIALLAILSVALLFASKWALDYLEMLARR
                     EGRLTERRR"
     gene            12043..>12779
                     /locus_tag="AC162G_07980"
     CDS             12043..>12779
                     /locus_tag="AC162G_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5075788.1"
                     /translation="MREALRSFKMATWLGWQIESNWTDPFLFGIYSIAKPVSAALILV
                     VMYSIVAQGGPGHPLFPYMYVGNAFYIYVGSVLIGISWAIVGDREEYGTLKYIAAAPL
                     AMLWYLLGRGMARTIIGTISVIVTLAIGVLFLQVPIALPIVEWPLFGVAMGLGLSGLM
                     AMGLLLAGVSFLIARHAGFIGEAVAGALYLFTGAIFPLEVLPGVLQPIGYVLPVTYWL
                     EAVRRALLGAHGGRTFAAWSDAQVVGAL"
BASE COUNT         2214 a         4068 c         4166 g         2331 t
ORIGIN      
        1 agcatgccag cattggaaac cgaacggttg atcattcggc cgttcgcgat ggacgatctg
       61 gacgcgattc accgcatcct cgacgtggaa ctggccgaag tggatttcgg cagcgaggag
      121 gcaaagacgc tggacgctcg gcgcgagtgg ctgcgctgga cgatcctcaa ttacgatcag
      181 ctcgcccggc tctaccagcc gccgtacggc gagcgggcca tcgtcctgaa atcgagcgac
      241 gagctgattg gcgcggccgg ctacgtgcca tgcctcaacg tgttcggaca gttgcctgtg
      301 tccggcggga cgcgcgacgg cctgacctcg accgagttcg gactatacta cgcgctgtca
      361 cccgcgcacc atcggcgcgg ctacgccgcc gaggcggttc gggcgatgat cgactacgcc
      421 ttccggcgac tcagactccg gcgcatcatc gccacgacga cgtataataa taaggcgtcg
      481 atgggcgtga tgcgcaaagt ggggatgcgt attgaaaaga acccgtttcc cgatccaccc
      541 tggctgcaag tggtcggtgt tttggaagcg cccagcacat aaagcgtgtt gcgtgttccg
      601 tagtccgtaa cggaacacgt gacacgcaac atggaaggga cattgaccgg taagcggaga
      661 gcgcgcagga aggtcatccg ccacaacacg gcggtgaatc aagagaagca tcgcgccgac
      721 tggctgccgc tggcgccgaa cgagctaggc gtggtggccg accggcgcat catgccgcgc
      781 gacgagcgcg accggcggcg cgcgctggaa gaagcctcgg cgcaaagcat cgacgcggcg
      841 atcggcgagg cagactggct ggcggcggcg caggcgagtg ggcatcgcct cggcgtcgtc
      901 ctcgaaatcg ccaaagacct gtgccgcgtc agcgtcgacg ggcgggcggt gctgtgcgac
      961 attcgcggca cgctcaccgc tgagggcacc ggcttcacca acatcgtcgc cgtcggcgac
     1021 cgcgtgctga tcagcctgcc cgaagccgat cacggttggg tcgagcaggt actgcagcgc
     1081 cgcagcgcgc tggcgcggcc cgattcgttc tactcgcacc tcaagcagat tatcgccgcg
     1141 aacatggatc aactgctgat cgtcgcctcg tggcgcgagc cgcacatctg gcgcgagctg
     1201 atcgacgagt atctcatcgc cgccgcgcgc aacaacctcg aagcgttcat ctgcattaac
     1261 aagatcgatc tggctgaaga catagccgaa tgccgtgccg ccgtgcaggc ctaccccgat
     1321 ctcggctatc acctgttgct caccagcgcc aagaccggcg ccggcgtgga cgacctgcgc
     1381 cagattttgc gcggcaaatc gaccgtgctg gcggggttgt cgggcgtggg caagtcgtca
     1441 ctgctgtcgg ccgtgcagcc cggcctcaag cttcgcacgc gagcggtcag cgaatacagc
     1501 ggcgaaggcc ggcacacgac gaagcaggtg acgatgctgc cgctgcgcga gggcggctac
     1561 gtcgtcgata cccccggtat ccgcgagatg gggctgcgcg ggctgtaccg cgacgagctg
     1621 gtcgaattct atccggagat tctcgctgcg tcgcccaact gccgatacag cgattgctcg
     1681 cacatcggcg agcaggtgtg cggcgtgaag tcggccgtcg ctgcagggcg catcgccgac
     1741 tggcgatacg acaactacag gaagctatgc gcgaaactgc cggagtaatt ggagattgga
     1801 gattggaggt tggaggttgg aggtcggagg tcggatggcg gcgtcatcca attacccatt
     1861 accaatcacc aattaccaaa tacgatctcc atccttcatc cggtataatc cctccgcccg
     1921 gaggggcgat gaccaaaacg ctgatcaaga acgtgcacgt gatcacgctc gacgagacgg
     1981 gcgccgttct gcgcaatgcc agcatcgcga tcgacggcag gacgatcctt gccgtcggcg
     2041 atgtgccgtc tgacttcgcg cccgacgaaa cgatcgacgg ctacaaccat gtcgccgtgc
     2101 cggcgttctt caacgcgcat tgccacgcgc cgatgacgta cgagcggggc tgggcggaag
     2161 acctgccctt cccgcgctgg ctgaacgaga aaatctgggt ggccgaatcg gcgctcacgc
     2221 cggacgacgt gtactggggc gcagcgctcg ccgcgtgcga gatgatccgg agcggcgtcg
     2281 ccgcgttcaa cgatcactac ttctatatgg accgcgtcgc cgaggtcgtc gagcaatcgg
     2341 gcctcaaggc cgggctggca tggtgtgtgt tcggcatcgg cgacgacaag gagatcggcg
     2401 cggatctcga cggcacgctc gccttcgtcg agcgctggca ggaccgcgcc ggcggccggc
     2461 tgcgcctgat gctcggcccg cattcgccct acgtctgccc gcccgatttc ctggcgcgcg
     2521 tcgccgcgat cgcgcacgag cgaggcctgc cgatccacct gcacgccgcc gagtcgcagg
     2581 ggcaggtcga caactcgatg catgcgcacg gcaagtcgcc ggtcgcgcac ctcgccgcgc
     2641 tgggtgtgtt ggacgggccg tgcatcgttg cgcactgcct ggtcgtcagc gatgaagatc
     2701 tggacatcct tgcgtggcac ggcgtgagcg tggctcacac gccgatcacg tacatgaagc
     2761 tggcgatggg cgtgaacgat ctatcgcgct tcttcgccca cggcgtcaac gtcgccattg
     2821 gcaccgacgg gccggcctcg aacgccgaca tggacatgct cgctgcgatc cgccagacgg
     2881 cgatcctgca aaagcatgag cagcgcaatc ccgaaacgct gcccggcgac acggcgctgc
     2941 gcatggcgtc gcagaacggc gcgcgggcga tgggctttcc cgattcgggc gcgatcgcgc
     3001 cgggtcgcgc ggccgacatc acactcatcg acacggaccg cccacacttg ctgccacgcc
     3061 acgacctggt cgccaacgtc gttcactcgg ccaaggccgc cgacgtgacg cacctgttcg
     3121 tcgacggacg gttgctgtat cggaacggcg aaatcctgac gctggacgag gacaagatca
     3181 agaccgaagc ggagcgcggc gcgtttcgca tggtcggcag cgagatgcgc cgtgtgcgcg
     3241 aatatctcgg ctagtaacta ctcagcccaa cgtcattgcg agcatctttg tgcgaagcaa
     3301 tcccccggcg gccaggagat tgcttcgtcg caaaagacgc ttacctgccc cgcgcttccc
     3361 agcggggtcg caatgacgcc tgagttgtta gcggcggcca catgatctac gtcgagtgag
     3421 gtcagggcag agacttcgga agtcttcaaa gacttccgaa gtctaaggcc aagtgcgtcg
     3481 cacgtttcgt ccggctatct cactttcggc ctgcctttgg gttcgacgac ccgcagcggc
     3541 accgacgcga ttccgcccgg cggcacgtta ggcgcgcccg gactgggtga ccaatcgtcg
     3601 tgccacacgc cgtactcgtc acggctgaag ctgcggtccg cgccgagcgc gccgaagatg
     3661 accagctcga gcacctggcc gccaggcctg agcaggcgca cgccgtcgcc gttgtcgttg
     3721 agcgggatgt gggtcatctg gcgatagagc accaggaact tgccaggccc tagcaccgtc
     3781 ccggccggga actcgtaggc cgcgccggcg gtggcgctct cgatcgacca gccgccgatc
     3841 tcgaccgtaa tcacgccggt gttagtcagc tcgatccact catcctgcgg gttggccgtg
     3901 ccgctgccgt cccaatccac tgcgccgggc gcaggcagga tctcgttgag gcgaacgccg
     3961 gtgagcgtcg gcggcgttcg cgtctgagtc gccgtggcgg taggcgtcac gctgggcggc
     4021 gcttgtgtag ccgtggcggt aggcgtcacg ctgggcggcg cctgtgtagc cgtcgcagtc
     4081 ggcgtcacac tgggtggcgc ttgcgtagcc gtcgccgtag gcgtcacgct gggtggcgcc
     4141 tgcgtggccg tggctgtcgg cgtggccgat accgtcggcg tcgcgctggg cgtgaccggc
     4201 acagtagtcg gtgtagccga tgctgtcggc gtcacgctgg gcgtgaccgg cgcagtagtc
     4261 ggggtcgatg tggccgtcgc cgtcggcgtc cgcgtaggaa aagctgtagc ggtcggcgtc
     4321 ggcaatcgct gccacacgac ggagatgcgc gccaggccgc cgttctcgta gtattccacg
     4381 acgatttggt gcgcgccgcc gagactcacg tcggccgtgt agggcgtatc accgacgctg
     4441 tcgtgccatt cattcaacac cagcctgccg tccacggtga tgcgcacgcc gtcgtcagag
     4501 atcgcgacga agcggtacac acccggctcg aagctcaact gccggctcca gcgcgccgag
     4561 aacccgtcca caggcaaccc aggcgcgggc gagccgccct gccagttgaa gtcgatcgcc
     4621 ttgtcgttgc gcgtcagcgc cgggttcccg ctcaagttcc ggttcgacca atactcacct
     4681 ttccagtcgg tgaacgacgg ggtgagcttt tcccaccaca ggctgatgcg ggcgtcgccc
     4741 gtccgctcgt agaactccac tttcaggccg tgaaagccag cggccatcgt cacgtccgca
     4801 gtgatctctc gcgccgcgcc gtcgcgccaa tcgtcgatga ccaggccgcc gtcgacccac
     4861 aaccgggtgc cgtcgtcgac gaggatgtgg aaccgatagg tagccgcttc gaagttgagg
     4921 ctgcgcgtcc agcgcgccga gaaattgtcg gcgggcacga cttgggccgg cgtgccagcg
     4981 ccccagttga agtctaccgt cttgtcattg cgcgtgacga ccgggccgcc gctcagcgtc
     5041 cgattcgacc agtattcacc cttccaatcg gggtacgtgc taaacttctc ccaccagacc
     5101 tggaggagtg cgctgcccac gcgctcgaag tactcgacgc gaagcgcgtg gttgcccgca
     5161 gccagcagca cgtccgctgc gacttcacgc acgccgccct cacgccactc atcgacgatc
     5221 agtgcgccgt cgacgtacac tcggacgccg tcgtccacca gcgcgcggaa gcggtacgtc
     5281 ccttcgtcga accccatcgt gcgcgtccac cgtgcggaga agttgtcggc gggcagagaa
     5341 gcatccggcg cgccgccgcc ccagttgaag ctgatgctcc cgtcgttgcg cacgagcttt
     5401 gccgcgcccg tcaggctggc attggcgtaa tactcgccgc gccactccgg gaactcgccg
     5461 ggacgctccc accagaactg gatctgggca tcgcccctgt tttcgtagta ctcgacgcgc
     5521 accgcgtgca ggccagcgct cagcgcccga tccgccgagt aggtgacgtt cgtcgcatcg
     5581 tgccactggt cgacgatcaa ctcgccgtcc agccacacgc gcacgccgtc gtcgctgcgc
     5641 acgtagaagc ggtagacgcc tgcggcaaat ccgagcgagc gtgtccaccg cgccgagaag
     5701 tcatccgccg ggatcgacgc cgccggcgag aaagcgcccc agttgaagtt gacgtcggga
     5761 tcgccgcgca cgagcgccgg cgcgcccgac aggctccggt tggcgtagta ttcggcgcgc
     5821 catccgatga ccgccggcgt gggcgtgggg ccgacgacca cctgcaccca gaacggctgc
     5881 tgtccgtcgt cgccgatgcc gaacaggacc ctcttgccgt cgcgcagcag ccagttgccc
     5941 cgatacgtgc cggcggcagc cggcgcaacc aggctcacgg acacgtcgac cgtggcgccg
     6001 ggcggcacag acccgggcag gggccatgtg gccggcccgc ccatggcgtc gccgccggcg
     6061 aacaccagcg cgtaggacgc gtcccaggcg caggttccgc tgttgcgaag ccgccaggct
     6121 ttgataaacg aagcgccggg cgcgacgacg gtcttatcag gaatcgtcag gtcgctcacg
     6181 aaggtcgccc gattggagca ggcggcgcct ggtggcgtcg gcgagactgt tgcgccgggc
     6241 gtcggctcgg tcacgggcgg ctgaaacgcg aagtcggcgg tcgccttcga gacgaagtcg
     6301 tccgtcgccg cgacgacgac gagccgatcg cccgtgatcg gcgtgccgtc tgcccaggcc
     6361 ccgggcacga agaccacaat gttgatcgcg ccgttcgcgt cggcgatggc gtcgccgaat
     6421 ggctgaagcg ccgcgccggc attcggcggg ccgaagcgaa tggcgacgtg tgcgctcgcc
     6481 gggaaaccca cgccgacgat ggtgaccggc gtgcctgggc cgccggaggc cgggttgagg
     6541 gcgatgaccg ctggcgtggc cggcggcgcg gttggagtga tcgtcggttc aacgctcggc
     6601 gcgatcgtgg gttcaggtgt tcgctgagcg ccgcacgccg acacgatcgc ggccgcggcg
     6661 aacagcgcca tgatgagaag aaatcggttg aagaaattgt ggatattcat gttaaccccc
     6721 tgcagctcaa gcgacatcga ctgcattcaa ctactggaac gcctgcgcgt atcttccggt
     6781 tccacgtcgc gccggcgggc tggatggcgt cgatttaccg tagtgtaatc gacggcgcag
     6841 ggggtgcgca tcgcacaaat gatgtcgatc gtctacatca ttcgccgttg accgcctacg
     6901 acaaaagatg gagaagcagc ctcgttctat ctacgattca caccacacga taccacacgc
     6961 cggtgctttc cggcaggatg gcgagccgct cgcgtgcaac tcgatcggcc agcgcgtcgc
     7021 cgaaaaagaa gcgcgccagc ctctccgctt cttcaaccga ctcgaaccgg taatcggtcc
     7081 gtatccatct tgatcgaaag tcatgctcct cttcgagcag cctgtagtat tcagccagcg
     7141 catcggtcgg cggctcgggc atttcacgcc cggttccgag cgtctccagg atgatcgccg
     7201 tgccgccagg tcgcagaacg cgtatcatct gcgcgacggc aagtctgacg actttccgcc
     7261 agctatccgg ataccagccg gtggcatgcc cgaagctcca acccgagacg caaatgtcgg
     7321 cgatgccgtc gcgagcaggc aggcagcaat tgtcggccac tgccagaagc cagttgcgcg
     7381 caggcagata gggcagcttg ccggccgcca cgtcgagcat ggccttcgat gcgtcgaacg
     7441 cggcgatcga tcgagcgtgg ggcgcgatca ggcgcgtcag ccggcccgtt ccggcgccca
     7501 tttcgacgac gtccaggccg tgcagcggcc ggatttgccg cagtgtgcga aggatgttgc
     7561 gctgataatc ctcgcgcgtc actaatgcat catactccgc ggcccgctgg cgatagatcc
     7621 attcgtaatc cggcatcgct ctaacgttac cacagagctg aaggcgagtc tttgacgaag
     7681 ttgagtgcgc cctgcccgtc atcgtgcgag atagtcaggc ggtcacccgc aagctcaaac
     7741 cgagcgctgg cttccaaggc ttggaagtac cgctgctcct gctccatcac gcgctcgtcg
     7801 gcgcaagcta tcagcgtgct cacgatgttg ctgatcgaaa gcgcggtgcc gttcacctga
     7861 tactcgcctc cgaacgagtt gcagcctcct gagccgccga cttggccatc ggcgccgaac
     7921 tccagggtga gcgaggtgcc ttcaatgacc ggcgattctg cgcccgcgac accaaaggac
     7981 accagccgcc agttcgtgtt ggctaaggcg tccgtagcgc ttggcggcac ggttggctcg
     8041 gcagtgggtg ctggcaccga tccactcggc gcgcagcctg acgctatcag gatgatcgcc
     8101 agtagaaaca tcagatttga aagcttcacg ttgtcctcct ttcttgctgt tccgtcatca
     8161 ggtgcggacg cgcgacataa ttcgtgcgca cgcggggaat gtagagcttg gtcgcaagaa
     8221 gcggcgtgac agcggacatc atgacttcct tggaccaggg acaccagaat tatactgacg
     8281 ggcgatgggt tgagcaagtg ggggaaggag tatagcgctc ttgattgaaa ctcatgtaga
     8341 gttggccgcc tccagaaact aacccggatg ccaaggctat ttggaatctt caaaggtatg
     8401 cgatacaatc cggcgcgttg tgtcacatcc ggacacagca cgaaggagct tgcatgcgca
     8461 accgcctctc acgcatcgtc ttgagtctcg tcgtcggatc gatcgtggtt gcgatcgggc
     8521 tgtacctggg ctggttcgtc gcggtcgggc tgcaggccgc gcgcacgttg agcggctcag
     8581 tccgggatca gccgtctcgc gagacagttg ccttagtgac cgacgtgacg cgctggacgt
     8641 ttctggggct gacgacgggg ttgggcctgg cgttcgccgc gctccacaag cgccgcttgc
     8701 gatttctcct cgcgtcgatc gctggcttcg cgctgggagg ggcgctggct tcatggcttg
     8761 tcgtgaccag tctcgatcgc gccgcgccgg cggcgacgtt ggcattgcca ctgggcggcg
     8821 cgctcgccgg gctgctgctg gggcttggag cgcggctgga tattcggtcg gcactgatgc
     8881 tcatcgtcgt ggcgctggcg atgatgatcg ccgggccgtt catcgatccg cgcagctccg
     8941 ttgtgctgcc atcgccgctg cgcctgacac cgggcgactg gctcatcttg ctcgcgcccg
     9001 gcgcgctgat cggcgccatc ctcgccgcgc tcgtctcgaa cggctccgac acatcgcaaa
     9061 ccgcgtgata ggcgcgcggg tcttcacgac cgtttttgag atgatttctt agtggacggg
     9121 tgactggggg tcgtcggctg ctgaggggta tcgatccaga gcggcgcgac tgaacacgcg
     9181 agggagcacc ccgtgcacgc gcgcatcgtg cgcccggcgc agatgctctt ctgcgcgcgc
     9241 gagtgcggcc tcggcggaag cgagaagggt gtcgagccgg gcaggcgccc ctccagccgg
     9301 acgatacgac gcgctcgaga tgaagacgac gtcacgcatc tcttttcctc cgagtggggt
     9361 aactgctcac gcaatcaaaa gaggcgaagc atcccagcga caagatcccc actcccttcg
     9421 cagggcatac ctcgactccc gctttgactg cgctcagggt ccgctctggg cgcttgcttc
     9481 gaaagatcgt gagtagttat cgcgtacagt aaacctgtgc agtataactc caaatcactc
     9541 caattgatac gtgactcagg tcatagcccg gtatgagttg cgttagggtt gcgtatggat
     9601 cgcgatagaa tgatcgcgcc acaatctttc gcacaccggt cgaacgtgat tcgatgcact
     9661 gctcacacga tactgtcact tgccatagac attcgtaaga gcttgttgga acgcagatga
     9721 ccgaagtctc atagttaatt cttacaggtg attgcacaca tcttttgcct gatgctcgag
     9781 tctcggtcgt ttcttgactt tctccctgaa tgggtgtaaa gtcgcgccat cctgacgaaa
     9841 ggaggcgcgc tcatggtgaa gcacattcgc accggcgatg catatcgcac cgcatctacc
     9901 agcggtgtcg tttcaccttg tcggagtgcg cctgatgacc gttaggtctg tcgtattgcc
     9961 tgtacggcca cgggtaggca cacccgtggc cgttttattt tggtcgacgt tcgaccggac
    10021 aaaaggtcag tagacgactc agaacatctg tgctattctg agatggctga gttacaagaa
    10081 gccaaagggt tgagaatgcc atgaacgaat cactcgctat cgagacggag aaactgggac
    10141 gcatctacaa gattcgcggc gcgaagaagg gcgaagcgcg cgagctcgtc gcgctgcaag
    10201 acgtcgatct cgaagtccgg cgcggcgagc tgttcggtct gctcggcccg aacggcgccg
    10261 gcaagacgac gctgattaag attctcacca cgctgctcct gccctcgtcc ggccgcgccc
    10321 tggtcgacgg gctggatgtc gtgacgcagc acaggctcgt gcgcgagcgg atcaacatgg
    10381 tgtcgggcgg cgagtcgtcc ggctacggcc tgctgacggt gcgcgagaac ctgtggatgt
    10441 tcgcgcagtt ctacggcatc gatcacgcga ccacctaccg gcgcatcgac gagatgctcg
    10501 aggtcgtcgg cctgagcgat cgcaagaaca cgaagatcag cgacctgtcg accgggctgc
    10561 gccagaagat gaacatcgtg cgcggcttca tcaccgatcc gcagatcctc ttcctcgacg
    10621 agccgacgct cgggctggac gtcgccgccg cgcgcgacgc gcgccagttc gtgcgccgct
    10681 ggataggccg gcgggacaag acgatcctgc tcacgaccca ctacatggtc gaggccgatg
    10741 agctgtgcga tcgggtggcg atcatcgacc gcggccgggt gctggcctgc gacacgccgg
    10801 cgagcctcaa gcgccgcctg cagcgcgacg cgctcttcca catcgagacg tcgtaccttt
    10861 cgaccggcgg cgcagacggc tttgaagcga tacccggcgt gagcaaggtg acgcaccggc
    10921 atcgcgacga tcggactgca ttcgagttcc tgctccaggg cgaggacgtg ctcgcgcccg
    10981 tcgtcgcgcg catgaccgag cagaacgcga agatcctctc gttacagaag cgcgagccga
    11041 cgctggaaga cgtgttcatc gacctggtcg ggcgcggcct gaccgacggg gagaacggca
    11101 atggaagcgg caatcactaa cgcaccgacc cggccgccgt tcaagcgccg cgccaacttg
    11161 ttctggcgca cggtcttcgc gcgctcgtac ccgcgcctca tcggcctgcg gcgcgagccg
    11221 tcgtggatgt tcttcgagat cgcgctgccg ttcctgatcg cctgctcgta cgtgttcgtg
    11281 tacaaggcga tcggtgcgcc gcaggaattc atcggcttcg tcgtgctggg cggcgcgatg
    11341 agcgcgttct ggctgaatat gctgtggatg atggccagcc agttgttctg ggaaaagcag
    11401 tcgggcaacc tgccgctgta catcgccgcg ccgaactcga tgacggcgat cctgctcggc
    11461 atggcgctgg gcggctcggc ggcgacctgc gtgcgcgccg ccgtcatcgt cgtcaccggc
    11521 tcagtgctgt tcggcgtgac gtactcgacg gtgaatctcc taccggcgct cgccgtcttc
    11581 ctgctgacga tggtcgcgct gtacgggctg ggcatgctgt tcgcctcggt gttcctgctg
    11641 ttcgggcgag aggcgtggca cacgagcaac ctgctccaag agccggtgta cctggtgtcg
    11701 ggcttctact tcccgatcaa gagcctgccg gtgtgggttg ccgccgtcgc gtcgctcgtc
    11761 ccggtcacgc tcgggctgga cgccatgcgg caactgttgt tcgccggcgg cgaaacgatc
    11821 gggttcctgc cggtctcgat cgagatcgcc ctcctggcca tattgagcgt cgcgctcctg
    11881 tttgcgtcga agtgggcgct ggactacctg gagatgctcg cgcgcaggga aggcaggcta
    11941 acggagcgaa ggcgttgagt taacctacag aagttccctc cagttccttc agttccttga
    12001 gttccaggga actgaaggaa ttcgaggaac tcaggagata acatgagaga agccttgcgc
    12061 tcgttcaaga tggcgacgtg gctcggctgg cagatcgagt caaattggac cgatccgttc
    12121 ctgttcggca tctactcgat cgccaagccg gtgtcggcgg cgctgatcct ggtggtgatg
    12181 tacagcatcg tcgcgcaggg cgggccgggc catccgctct ttccttatat gtatgtggga
    12241 aatgctttct atatttatgt cggctcggtg ctgatcggca tctcgtgggc gatcgtcggc
    12301 gaccgcgaag aatacggcac gctcaaatac atcgccgccg cgccgctcgc catgctgtgg
    12361 tatctgctcg ggcgcggcat ggcgcgcacg atcatcggca cgatttcagt gatcgtcacg
    12421 ctcgccattg gcgtgctctt cttgcaggta ccgatcgctc tgccgatcgt cgagtggccg
    12481 ctgttcggcg tggcgatggg gttaggcttg agcggcctga tggcgatggg cctgctgctg
    12541 gcgggcgtgt cgttcctgat cgcgcggcac gccggcttca tcggcgaagc cgtcgccggc
    12601 gcgctgtacc tgttcaccgg cgcgatcttc ccgctcgaag tgcttccggg cgtcctgcag
    12661 ccgatcggct acgtcttgcc ggtcacgtac tggctggagg cggttcgccg cgcgctgctc
    12721 ggcgcgcatg gcgggcggac gttcgccgcg tggagcgatg cgcaggtcgt cggcgcgct
//
LOCUS       DBNHXB010000197        22681 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_715170, whole genome shotgun sequence.
ACCESSION   DBNHXB010000197 DBNHXB010000000
VERSION     DBNHXB010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 22681)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22681)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22681
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_715170"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="AC162G_07985"
     CDS             <1..812
                     /locus_tag="AC162G_07985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR5075789.1"
                     /translation="AIVARFFDAGPLAPVITESLKFAAIAFIFTRYRREFDNILDGIV
                     YGALAGIGYSMTINLLTYATGFLFGGFELLGTSVLLNGIAFGLNEAYYGAAIGIGFGV
                     SRWARDRRLRIAAPFIALAAAIGLHLFNDYWRDLAVGDRSGLVIIPILATWAGIVAVV
                     AIAVLSLRRDQEIIRAHLKAELDLRTLTPNEFFYLSTPRNRSRFLLQAARRGPAELAR
                     ALQMRETITQLAYRRRELGLLGRDAATDAVAASLRQRVARLRPKPRPRENR"
     gene            863..1270
                     /locus_tag="AC162G_07990"
     CDS             863..1270
                     /locus_tag="AC162G_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075790.1"
                     /translation="MNADKHGLHRSRWLPLIVLLAILGAGCRSDPNDQFIQGWWTYSD
                     PHLGSIVSEKFQESVWRFDRGTYRFDACCMFEQHTFGKYNIVESEGDVLVLELFWQDG
                     SYRSEPTQIRIVIDRQNDTLKVQRTGPFRRITP"
     gene            1462..2490
                     /locus_tag="AC162G_07995"
     CDS             1462..2490
                     /locus_tag="AC162G_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5075791.1"
                     /translation="MRYKLLGRSGLRVSELCLGTMTFGEDWGWGASKEVSREIFDAFA
                     EAGGNFVDTACNYTNGTSERFVGDFVTSDRDHFVVATKYTLSERKDDPNFGGNQRKNM
                     MRSVEGSLKRLKTDTVDLLWLHMWDFTTPVEEVMRGLDDLVRSGKVLYVGLSDSPAWI
                     AAQANTLAELRGWSRCIALQAPYSLADRAVERELLPMARACDMGVLAWGVLEAGELTG
                     KYNRESPEPKRNRSAGEMNKALADVLMALADEIGRTPSQVAINWVRQHGNGHAAPIIP
                     ILGARTEAQIKDNLGCLEFTLTGEQLRRLGEASPISLGFPQAFLSDDHVRNLIFGETF
                     DRLDNHRA"
     gene            2503..3273
                     /locus_tag="AC162G_08000"
     CDS             2503..3273
                     /locus_tag="AC162G_08000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase family protein"
                     /protein_id="HIR5075792.1"
                     /translation="MIDAAAYLRRIDYDGPLAPAIDTLRGLHGAHLLAVPFENFDIHF
                     GRPIVLDEARFFDKIVGERRGGFCYELNGLFAALLHALGFDVTLHSARVREGNGRYSP
                     EFDHLVLVVYLEERWLADVGFGDSFRLPLRLDERGDQVQDGHIYRIRDEGHGLTMLEY
                     GEDGAILDGYAFTLQPRRLEEFAGMCRYHQTSPESSFTQKRICSRATPLGRVSLSDNR
                     LIITERGERREVELGSEEEYAAALRECFGIELTPYLST"
     gene            complement(3420..4349)
                     /gene="secF"
                     /locus_tag="AC162G_08005"
     CDS             complement(3420..4349)
                     /gene="secF"
                     /locus_tag="AC162G_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR5075793.1"
                     /translation="MWDIVGRRYWYFAFSLLVTVPGLIALLIGLLGGGLPLGIDFTGG
                     SLLKIRFDDTPPQPGDIKAIYAEFDFDDSQVQTLEDNGIQIRSRAMDDATKNEIAAVI
                     GDRHGAFTELEFSSVSATIGAEVIQGAVQTIGIATVVIMGYLWWAFRRVPHAFRYSVA
                     AILGLLHDVAVIVGMTALFGQFLGWQVDSLFLTALLTMLGFSVQDKIVVFDRIRENLL
                     RRQGGRYEQIVNLSIIQTVARSLVTQLTMFFTMLALALFGGITIRNFIVIMLVGLLSG
                     TYSSIFNAAQILVVWENREWRTWFRRRPAGQAA"
     gene            complement(4398..5861)
                     /gene="secD"
                     /locus_tag="AC162G_08010"
     CDS             complement(4398..5861)
                     /gene="secD"
                     /locus_tag="AC162G_08010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR5075794.1"
                     /translation="MASRTLVWLVLTIVLALAAAFVSLASSIPVKLGLDLSGGTQVLL
                     EAISAPGQTLDDAAMERTRQIIENRVNAFGLTEPVVQRAGSNRILVELPNEKDPARAV
                     STIQQTGQLEFVEAGFEFFPDGAILRTSTSPTPTNVLTATEGLTGTNLLNPTPPATAT
                     PVVTDTSALTSTTPITPAVPTVDPLEAQFGPIYPTLITGADLRPESLQLDEDEFGRPA
                     VAFEVVADKVSVLQEYTTVYQPTSSRPGGYLCIVLDNRVQSCPQIQSALPGRGIITVG
                     GDVEQAQSLLTLLRYGALPVSMQVVSSNTVGPTLGEDSIRASVIAGAIGLSAVAVFML
                     LYYRVAGLVAVLALMLYGLVTFALFKLIPVTLTLPGIAGFILSVGVAVDGNTLIFERM
                     KEELRSGRRLGVAVDVGFERAWTSIRDSNVSTLITCVILFIFGSQFGASIVKGFALTL
                     AIGVFLSLFTSIRVTRMLLHLITDNVSLEDRPHLFGA"
     gene            complement(6089..7168)
                     /locus_tag="AC162G_08015"
     CDS             complement(6089..7168)
                     /locus_tag="AC162G_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR5075795.1"
                     /translation="MASRLRTHSTRRRVRAGLRDTWVVLREFRGPIFVFLVVLVGAAV
                     LYRVLALAAHAQDPAVDAPSIPESIYLMLSMIFLQAGAEFPEPWYLEIFFFAMPVIGL
                     ALLGSGVANLGVLLFNKSARGSAWEAALASTFSNHVIVVGLGKLGYRIVQQLLDFGQD
                     VVAVEVEPDKPFVSLVRKLGVPVIIADARHGDALKSAGIERATSIVCCTQDDLTNLDV
                     ALDAREQRPGVKVVLRMFDADLAARVERGFGIHTVFSTASLAAPAFAAAAARAHVEYS
                     FYVDDQLLHVARVAVEPGSPLAGKAIEQVERQHDLTIVLHGRPNEKHLHPRSDEALGA
                     GDTVVVVASLEVLGQLGLISARPDR"
     gene            complement(7190..7837)
                     /locus_tag="AC162G_08020"
     CDS             complement(7190..7837)
                     /locus_tag="AC162G_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014643.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR5075796.1"
                     /translation="MKIVVSVHELATETQLVIDNMRRSNAPVLIGDPNKPVAVLIALE
                     EYDRLLREAQKASAAAAAAASAGAAAPVRDTTQPLPASKPAMAEAEASPAAPVRPAHA
                     EVLPASQPANIVQRAAAAIQANPAAPAQPKVVSQRGTTLQPDPTLAAGRAAPALRSRL
                     SKPLPRPPQPLRTPRSRPQLSLASIPGGWQTVALIAGMLALGIVGFALIIGAVGG"
     gene            complement(8118..9311)
                     /gene="menC"
                     /locus_tag="AC162G_08025"
     CDS             complement(8118..9311)
                     /gene="menC"
                     /locus_tag="AC162G_08025"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HIR5075797.1"
                     /translation="MSNLQPPTSDLHIERIELRLVRMQLKAPFRTSFGTEHERECIIV
                     SVHGGGLTGWGECVASRGFAADPFRATTGTGGASRLDWHLYSYETTVTAWHILRDFLV
                     PQVLGRPLSIDDMVDAGQQLRGHPLARAGLEAALWDLTAQLEGIPLSRALGGTRERVS
                     VGVSVGIQPSYEALVDTVDAYLAQGYRRIKIKIKPGYDLEAVKRIRAKHPDILLQVDA
                     NSAYTLADAPLFEAMDEHKLLLIEQPLGWDDLYEHSKLQPRLRTPICLDESIHSLGHA
                     RLAIELGAARVINIKAGRVSGFTESRRIHDLCQSRGVPVWCGGMLETGIGRAGNVALA
                     SLPNFTLPGDVSASDRYFHEDIVDPPFTLNSDSTISVPISPGLGVAVNLTRLDAVTVR
                     REAFV"
     gene            complement(9398..10306)
                     /locus_tag="AC162G_08030"
     CDS             complement(9398..10306)
                     /locus_tag="AC162G_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075798.1"
                     /translation="MTDSFSIRPLKTIDECRQAEELQRVVWRLDDLDVTPSHVLLTFA
                     KNGGVVLGAFVVSGGREHMVGCVFGFIGTREGLYAPEAPAQVKLKHCSHQLGVLPEWQ
                     SRGVGYALKLAQREAVRSQALRLITWTYDPLESRNANLNIAKLGAVCDTYIPNLYGEL
                     RDDLNRGLPTDRFQVDWWIASKRVETRLSRRRPPLTRALYQRAGTRILNEATFDERGL
                     PVPPDAIEPLHDDRALVEFPALFQDVKRLDTQLALAWREHTRAIFQPAFAAGFTVIDM
                     LYERDVTPRAFYVLTRTESSEASPAD"
     gene            complement(10380..11318)
                     /locus_tag="AC162G_08035"
     CDS             complement(10380..11318)
                     /locus_tag="AC162G_08035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5075799.1"
                     /translation="MILITGATGLVGRQLVARLAGREDVVCLVRPARRVRQFAPGVSV
                     RVVSGDVDDVPTLRLAMHSVTSIVHLAATWQPYSSGRTIESVNVGGTQNVIEAAREVG
                     VEHIVFVSPIGADPHSAYPYLRCKGQAEELIRTSGLAYTTLRASAVYGEDDKWTMPMA
                     MGMHALPFVFPIPGDGRSRLQPLCVHDMVTCLIACLSDSKKIGQTIDIGGPQVMTFDE
                     AVAEIMNAAGVKRRRFHLHPRVAHSLVRLSSRLLGRPPFSNSAVDLLAVNRTTALDAV
                     PFHFGFQAARMSDSLDYLKRPRPWRRMFVRYLMARE"
     gene            complement(11404..11796)
                     /locus_tag="AC162G_08040"
     CDS             complement(11404..11796)
                     /locus_tag="AC162G_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075800.1"
                     /translation="MDIILTAHTYLRLLILTLGALGILRALVSLGTRNATFMRVDEVL
                     SRGYTGALDLQAAVGAILALMLLAQSDSVRWIHPIIMLPAVFVAHLGRRFQDRPDRER
                     QKAQLAVYAGSLALVAVGLAVIGELRLA"
     gene            11986..13254
                     /gene="fabF"
                     /locus_tag="AC162G_08045"
     CDS             11986..13254
                     /gene="fabF"
                     /locus_tag="AC162G_08045"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR5075801.1"
                     /translation="MRRVVVTGMGALTPVGNDVDTMWASLVAGKSGIAPITHFDPSAF
                     ETRFAGEVKDFDPVALLGRKEARHMDRFTQFAMAATGQALADSGLKITPDNTLRVAVI
                     IGSGVGGISTIVGEVNVLRDRGANRVSPFLVPMMLSDTAPGTVAIAYGIRGPNLSIVT
                     ACATGTNVIGEAAEMIRHGRVDAAIAGSAEAGIIPLAMAGFNSMGALSRRNDQPEKAS
                     RPFDKNRDGFVASEGAGVLILEALEHALERGASIYAEVIGYGSSADAFHITAPEENGA
                     GAAAAMRMALDQAGLPPSDIDYLNAHGTSTLLNDKSETAAIKTVFGEAAYDVSISSTK
                     SMTGHLLGAAGSVEAIACIKALQEGIIPPTINYETPDPECDLDYVPNTARRKVVRRAM
                     SNSFGFGGHNACLILASVDNRGADPTSNVS"
     gene            13275..14447
                     /locus_tag="AC162G_08050"
     CDS             13275..14447
                     /locus_tag="AC162G_08050"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR5075802.1"
                     /translation="MAEFRNGRHAHIVGWGKYVPSRVVTNADLSRTVDTSDEWIVTRT
                     GIRERRVAGPKDTSASMGLAAAREALERAGVRPHEIDLIVVATATPEYAFPATACVIQ
                     DALGAVNSGAFDLSAGCTGFMYALALATQSIQSGAHNIVLVIGTETLSRIMDWNDRAT
                     CVLFGDGAGAIVLRASGQNGGVMSTLCRSDGSGGELLIIPGGGSKHPVSHQTIDQGLH
                     FIRMNGREVFRFAARIMDKSAREVASKAGLTMDDVQLVIPHQANLRIIQAAARALKLP
                     EERFFVNLQYYGNTSSASIPIAICEAVEQGKLKRGDHCVLVGFGAGLTWGAAAIKWSG
                     PAPLHEPTRLERVYETLLYGLAGIRSRLVRVLRRIDSALFGSPSPGRIDKDGRRKK"
     gene            complement(14495..14836)
                     /gene="trxA"
                     /locus_tag="AC162G_08055"
     CDS             complement(14495..14836)
                     /gene="trxA"
                     /locus_tag="AC162G_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR5075803.1"
                     /translation="MHNEPIHVSDAAFEKVVLQSNLTVLVDFWAPWCGPCRMVAPSLE
                     KLAGEYAGKILIAKVNTDDNAEYAMKYGVQGIPTLLFVRDGQEIDRIVGAAPYPIIKQ
                     RLDAALSKVVV"
     gene            complement(14829..15143)
                     /locus_tag="AC162G_08060"
     CDS             complement(14829..15143)
                     /locus_tag="AC162G_08060"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR5075804.1"
                     /translation="MRPSELLLVALGACSAVDVVGILQKKRIDLRSLDIKISGEQDPD
                     PPWTYRKIHVEYILRGKGLTDRAVQQALHLSEKKYCSVSTTVSGVAAITQSYQILEDG
                     DA"
     gene            complement(15248..15514)
                     /locus_tag="AC162G_08065"
     CDS             complement(15248..15514)
                     /locus_tag="AC162G_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HIR5075805.1"
                     /translation="MNVSPEVKTDLLKRLKRLEGQVRGVQKMLDEDRDCREVLQQLAA
                     VRAAAHQASLVVVREHAAACLFKSGEQVSPQEVLDSLVSVLEKV"
     gene            complement(15562..15762)
                     /locus_tag="AC162G_08070"
     CDS             complement(15562..15762)
                     /locus_tag="AC162G_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR5075806.1"
                     /translation="MPIYEYQCEDCDERFEKLVRMSTRPEDVECPQCGARHAKRVVSL
                     MAAVGRGGSSASALACAPSGGG"
     gene            15938..17239
                     /locus_tag="AC162G_08075"
     CDS             15938..17239
                     /locus_tag="AC162G_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR5075807.1"
                     /translation="MSQLRKGNRDLIKEINRNLVLNLIKSKGPISRVELAKLSGLSPA
                     TISGIVADFTASDLVHEMGEGESRGGRRPVLLRLNHRAGFVVGVKLMDNAIASVLTNL
                     DAQVLYHRVVPLKVSPLHPTSGDERTSSTSGTETPSTVGVAKESILSAIIQTIEVTIA
                     ESEVDRQRVLGIGIGMAGVVDGETGICRYSPFFGWRDVQIAEPIAAHFKLPVYLENDV
                     NTLTIAEQWFGHGHGVDHFAVITTGRGVGTGIVVNGQFYRGAVGGAGELGHITLVEDG
                     PLCDCGKRGCLEALASDPAVISQARLAVAAGKPTILVEAKPLTLETIIAAAEGGDELA
                     RQLLADSGQWLGIGIATLANILNPKLVIVSGEGVRAGEWRFGPMRKALQDHTFNDLAD
                     GMEIIIEATGDETWARGAACVVLGEMFKSPVQREKAVDLMA"
     gene            17438..>22681
                     /locus_tag="AC162G_08080"
     CDS             17438..>22681
                     /locus_tag="AC162G_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013239.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HIR5075808.1"
                     /translation="MRQHKSFAWLSKLLIVALLLSSIEFALPTPVAFALSGVYHNPYG
                     QDDLYSTEPTERSPRDPQAGQTVYIKITTWPIEWGQAVWLTWTKNGVPQSDVGAQWKY
                     NSGNNSYWEAAFGPFQKGDDITYTVHANVNGCCERTTGPFSFTVTDWSTVTNVTGHTN
                     NTNYVRFTFGDSGGNFTPAMRLAFVSDQVFRVQFSPTGQGLNITGLNNYTVSEDATYI
                     TVTTSALQLKVQKNPYRLFVYKADGQTLIAKEYDPSVFRIFEWLTNGDTAVAKIGTHF
                     YTPTTEKFWGLGERYNLLNQRGKDVNIYTVDQYLDHGERTYLPVPFFINSANYGVYLN
                     TTYYSVFNIATYLSDMYGFTQNTSGSLSSTLDYYFIYGSTPNEIIQRYTDVTGKPKLP
                     PKWAFGPWMMANEWDKQSEAQTQIDNTDTYDIPATVLAIEAWSDEATFYIWNDAQYTP
                     QPGSYCFTYNDFTFPVGGKWPNPRQLVQNAHDKGIKVLLWQAPVEKWMSSPYTQKDND
                     ESYMLQQGYAVGDGSGGSYRIPGGRWFANSLVPDFTNSAATDWWTNCKRGYLFNMNGV
                     SIDGFKTDGGEYIYGRNLTFANGKKGDEMRNAYPGSYIGAFHDFVQTKTISNGILLSR
                     AGAAGAQAYPGYWSGDERSTFSAFQDSIRAGLSAGMSGVPFWGWDLAGFSGDIPTKEL
                     YLRSAAMAAFTPIMEYHSETSGDPNPSRARSPWNMEARLSDSQARTGFAKFANIHMNL
                     VPYSYSEAKQTSLTGVPMMRAMPLEFPTDATAVNQVFQYLYGSQLLVAPVETQGATTK
                     SIYLPRGEWVDLWNGGQHSGPMTVTYGVSNLETIPVFVRGGAILPLNLDANYTLGGYV
                     GNTVDNYTRLTFAMYPFGSSSFPWFDDPINTVKTISVTENYLNQQVMVSVPPVSQTVT
                     LLVNTTVPISVTVDGNALTQYTNFASFQVAATGWYYNTQNQDVYVKIGSSAATRSVTL
                     NGVNKAAYEAEFEALTNVSTNTDHPGYTGIGFVDGFETQNDAVTFDGIEVSKGATFDL
                     RFRYSAANAGGATRTIYVNGQKVGRITFPQTTNWDTWATASISGNLYQGRTNTVKIVY
                     EAGDSGAMNLDNLLLVALPIRPAREYYQEEAMLANGYAFAQLDARGSLYDLMYPFGIY
                     TGVVVDGADASGTQGVQVNIWGALGGVEVDGKTHWLNHGDQWTFSQQYITDTAVLKTV
                     ATHNTASVRVTQYDFIPDGITFPNTTGGQPIRGMYLKRLVVENLAGTARDMGLLYYAD
                     LNINGSTAQDSVSYKTAEKALYFYDGGDSGSGRTRTLAFGLRLKSPGSVITETYQLYS
                     GDAAGYLKQVASIPAAGSQEFDVLLVGATSTTTGADLYSSDIVPAINWFNSQTINSVQ
                     TTTENTWTTLINGATVLDTPDPVYDANYRRSLITTILTINKDTGAVAAGFHNGAYAYN
                     WPRDSVYAAMTLDRAGIHDLPAKQYDWLWNVAERDTSSNDIGGDGVFYRFWYQKYTMD
                     GVRAWEQPQVDETAVIPFGAKLHWDMTGDTTFRSTYYPMVKEAALVSSENNPHPGLDY
                     SESMHLMFSYNIWEDMWGMLLYSNANVVAGLDAAAVLAGLEGQTSDQNTFISRRDDIK
                     NTGILQSVSSSNTTSPGLYSSDYNRFYMTKDLKKWYTDPSNIWNAITTDVSMLGIVVP
                     FGILSADHTWVVNTVNELEPALTDSAETGAAIGGVVRYRQDQVSRYGSTYANYGDTYY
                     DGGPWMVATTWLSQYYLERANT"
BASE COUNT         4474 a         7189 c         7032 g         3986 t
ORIGIN      
        1 tcgccattgt cgcgcgcttc ttcgacgccg gcccgctggc gccggtcatt accgaatcgc
       61 tcaagttcgc cgcgatcgcg ttcatcttca cccgctaccg ccgcgagttt gacaatatcc
      121 tcgacggcat cgtgtacggc gcgctcgccg gcatcggcta ctcgatgacg atcaacctcc
      181 tgacctacgc caccggcttc ctgttcggcg ggttcgagct cctgggcaca tcggtgctgc
      241 tcaacggcat cgccttcggc ctgaacgagg catactacgg cgctgccatc ggcatcggct
      301 tcggcgtgtc gcgctgggca agagatcggc gcctgcgcat cgccgctccc ttcatcgcgc
      361 tggccgccgc gatcgggctg catctcttca acgactactg gcgcgatctc gcggtcggcg
      421 accggagcgg actggtgatc atcccgatcc tggcgacgtg ggccggcatc gtcgccgtcg
      481 tcgcgatcgc cgtcctgtcg ctgcgccgcg accaggagat catccgcgcg caccttaagg
      541 cagaactcga tctccgaacg ctgacgccga acgagttctt ctatctgtcc acgccgcgca
      601 accggtcgcg gtttctgctc caggccgcgc gccgtgggcc ggccgagctg gcgcgcgcgc
      661 tgcagatgcg cgagacgatc acgcagctcg cctaccggcg gcgagagctg gggttgctcg
      721 gacgcgacgc ggccaccgac gcggtcgccg ccagcctgcg ccagcgtgtc gccaggttga
      781 gacccaagcc acgaccgcgt gaaaatagat aaagttttcc gctgaagaaa ctacatggcg
      841 cgcgaagatt tttaggacac ggatgaacgc agataaacac ggattgcaca ggtctcgatg
      901 gcttccgctg attgtgctgc tggcgatcct cggcgccggc tgccgctcgg atccgaacga
      961 tcagttcatc cagggatggt ggacttactc ggatccgcac ctggggtcga tcgtgtccga
     1021 aaagttccag gagtcggtgt ggcgcttcga tcgcggcacg taccgcttcg acgcgtgctg
     1081 catgttcgag cagcacacct tcgggaagta caatatcgtc gaaagtgagg gtgacgtgct
     1141 cgtcctggag cttttctggc aggacgggag ttaccgctcc gagccgacac agatcaggat
     1201 cgtcatcgac agacaaaacg acacgctcaa ggtgcagcga accgggccgt tccggcgcat
     1261 cacgccgtaa cccccacacg cgccgtcgcc caggctgtgg gcgaggtccg ctccgcccga
     1321 tagtgggcga gacctgccag gtctaaggca taagcctctc cagcgcagac agcaagtaca
     1381 ggtggaacct gatccattgg acagcgcagt gaattgcgac tttgtgtatc atctgaagac
     1441 aaaggttcca agggaggtca cgtgagatac aagctgctcg gacgaagcgg cctgcgcgtc
     1501 tcggagctat gcctgggcac gatgacgttc ggcgaggact ggggctgggg cgcatcgaaa
     1561 gaggtcagcc gcgagatatt cgacgccttc gccgaggccg ggggcaactt cgtcgacacc
     1621 gcctgcaact acacgaacgg cacgagtgag cgattcgtcg gcgacttcgt cacctcggat
     1681 cgcgatcact tcgtcgtcgc caccaagtat acgctgtccg aacgcaaaga cgatcccaac
     1741 ttcggcggca accagcgcaa gaacatgatg cgatcggtgg aaggcagcct caagcgtctg
     1801 aagacagaca ccgtcgatct gctctggctg cacatgtggg acttcactac gccggtcgag
     1861 gaagtgatgc gcgggttgga cgatctggtg cggtcaggaa aagtactcta tgtcggcctc
     1921 tcggattcgc cggcgtggat cgcggcgcag gcgaacacgc tcgccgagct gcgcggctgg
     1981 tcacgctgca tcgcgctgca agcgccctac agcctggccg accgcgccgt cgaacgtgag
     2041 ctgctgccca tggcgcgcgc ctgcgacatg ggagtgctcg cgtggggagt gctcgaagcc
     2101 ggcgagttga cgggcaaata caaccgcgaa tcgcccgagc cgaaacgcaa ccgcagcgcg
     2161 ggcgagatga acaaggcgct cgccgatgtc ctgatggcgt tggccgatga gatcggacgc
     2221 acaccgtcgc aggtcgccat caactgggtg cggcagcatg gcaacgggca tgccgcgccg
     2281 atcatcccca tcctcggcgc acggacggaa gcgcagatca aagacaacct gggctgtctg
     2341 gagttcactt taaccggcga acaactgcgt cgcctgggcg aggccagccc gatcagcctg
     2401 ggtttcccac aggcgttcct gtcagacgat cacgttcgca acctgatctt cggcgagacc
     2461 ttcgaccggt tggacaatca ccgggcctga ggctcgtgtc gtatgatcga cgctgccgca
     2521 tatctgagac gcatcgacta cgacggcccg ctggcgccgg cgatcgatac gctgcgcggg
     2581 ctgcatgggg cgcacctgct cgccgtgccg ttcgagaact ttgacatcca tttcggccgg
     2641 ccgatcgtcc tcgacgaagc tcgattcttc gacaagatcg tcggcgagcg acgcggcggt
     2701 ttctgttacg agctgaacgg cctgttcgcc gcgctgctgc acgcactcgg cttcgacgtg
     2761 acgctgcatt cggctcgggt ccgcgagggc aatggccggt atagcccgga gttcgatcat
     2821 ctcgtgctgg tggtgtacct ggaggagcgc tggctggccg acgtcggctt cggtgactcg
     2881 ttccggcttc cgctgcggtt agacgaacga ggcgaccagg ttcaggatgg gcacatttac
     2941 cgcatcaggg acgagggcca cggcttgacg atgctcgagt acggggagga cggcgccatc
     3001 ctggacggat atgcgttcac gcttcagcca cgccggctgg aagagttcgc cggcatgtgc
     3061 cgctaccacc agacctcgcc cgaatcgagc ttcacgcaga agcgcatctg ctcgcgcgcg
     3121 acgcctctgg ggcgcgtctc gttgagcgat aacaggctga tcatcaccga gcgaggggag
     3181 cgccgcgagg tcgagctggg cagcgaagag gaatatgcgg cagcgctgcg cgagtgcttc
     3241 ggaattgagc tgacgcccta cctctcgacg tgagacagtg ggcgagcggc gttcagcgtg
     3301 tcatgccgag gaagaatgac gaggcatgtc tgcccccggc gcgccgcgct cgccggtcgg
     3361 aggagagatt cctcactgcg ttcggaatga cacgatacac cgaggaatga cacgccgcgc
     3421 taagccgcct gcccggccgg gcggcggcgg aaccacgtcc gccactcgcg gttctcccac
     3481 acgaccagaa tctgcgctgc gttgaagatc gacgagtacg tgccgctcaa caggccgaca
     3541 agcatgatga cgatgaagtt acggatggtg atgccgccga agagcgccag cgccagcatt
     3601 gtgaagaaca tcgtcaactg cgtgaccaac gagcgtgcca ccgtctggat gatgctcagg
     3661 ttgacgatct gctcgtagcg cccgccctgg cggcggagca ggttctcgcg gatgcgatcg
     3721 aacaccacga tcttgtcctg caccgagaag cccagcatcg tgagcagcgc cgtgagaaag
     3781 agcgaatcga cctgccagcc caggaactgg ccgaacagcg ccgtcatgcc gacgatcacc
     3841 gccacgtcgt gcagcagtcc caagatcgcc gcgacgctat atcggaacgc gtgcggcacc
     3901 ctccgaaagg cccaccacag gtagcccatg atgacgacgg tcgcaatgcc gatggtctgc
     3961 actgcgccct ggatgacttc cgcgccgatc gtcgcactga ccgagctgaa ttccagctcg
     4021 gtgaacgcgc cgtgccggtc gccgatgacg gccgcgatct cgttcttggt ggcatcgtcc
     4081 atggcccgcg agcggatctg gatgccgtta tcctccagcg tctgcacctg actgtcgtcg
     4141 aagtcgaact cggcgtatat cgccttgatg tcgcccggct ggggaggcgt atcgtcgaat
     4201 cgaatcttca acagcgagcc gcccgtgaag tcgatcccga gcggcagtcc gccgcccagc
     4261 agcccgatca gcagtgcgat caagcccggc acggtgacca gcagcgagaa cgcgaagtac
     4321 cagtagcgcc tacccacaat gtcccacata agtctcctgc atttcggaag ttggagattg
     4381 gagattagag attgggatca ggctccgaag agatgagggc gatcttcgag gctgacgttg
     4441 tcggtgatca ggtgcagcag catgcgcgtg actcgaatcg aagtgaagag gctgagaaac
     4501 acgccgatgg ccagcgtcag cgcgaatccc ttgacgatgc tcgcgccgaa ttggctgccg
     4561 aaaataaaga gaatcacgca ggtgatcagt gtcgagacgt tcgagtcgcg gatcgaggtc
     4621 cacgcccgct cgaagccgac gtcgacggcg acgcccaggc ggcggccgct gcgcaactct
     4681 tccttcatgc gctcgaagat cagcgtattg ccatccaccg ccacgcccac cgagaggata
     4741 aacccggcaa taccgggcag cgtcagcgtg accgggatga gtttgaacag cgcgaaggtc
     4801 accaggccgt acagcatcag cgccagaacg gcgaccagcc cggccacgcg gtagtagagc
     4861 agcatgaaca cggcgaccgc cgacaggcca atggcgccgg cgatgacgct ggcgcggatc
     4921 gagtcctcgc ccagcgtcgg gccgacggtg ttgctggaca cgacttgcat ggacacgggc
     4981 agcgccccat agcgcagaag ggtgagcagc gactgtgctt gctcgacgtc accacccacg
     5041 gtgatgatgc ctctcccggg cagagccgac tgaatctgcg ggcacgactg cacgcgattg
     5101 tccagcacaa tgcacaagta accaccgggc cgactcgacg ttggctgata cacggtcgtg
     5161 tattcctgaa gcaccgacac cttgtcagcg acgacctcga aggccaccgc cggccgcccg
     5221 aattcgtcct cgtccagctg tagcgattcg ggccggagat cggcccctgt gatcaacgtc
     5281 gggtagatcg ggccgaattg agcttccagc ggatcgaccg tcggcacagc cggcgtgatg
     5341 ggggtcgtgc tcgtaagcgc agaagtatcg gtgacgacgg gcgtcgctgt ggcaggcggc
     5401 gtcgggttga gcaggtttgt ccccgtcaga ccttcggtcg cggttagcac gtttgtcggg
     5461 gtggggctgg tcgacgtgcg taggatggcg ccatctggga agaactcaaa accggcctcg
     5521 acgaactcca attgcccggt ctgttggatg gtgctcacgg cgcgcgccgg gtctttctcg
     5581 ttgggtagtt cgacgagaat ccggttcgag cctgcccgct gcacgaccgg ttcggtcaga
     5641 ccgaaggcgt tgacgcggtt ctcgatgatc tggcgggtgc gctccatcgc cgcatcgtcg
     5701 agcgtctgcc ccggcgcgga aatggcctcc agcagcactt gcgtgccgcc gctcaggtcc
     5761 aggcccagtt tcaccggaat cgaggaggcc agcgacacga acgcggcagc gagggcgagt
     5821 acgatggtca ggacaagcca caccagcgtg cgcgaagcca tgcgaagcga tcactccagg
     5881 caagaaagtc gccggttggc ttggcaagca tcaccgtgcc gaccggcgcg gattgtgtac
     5941 ggcgcgcaaa tgaaagttcc cacctgctcc cagcggattg ccaaatccgc cgctggattt
     6001 gacaatccgg cgggagcatt cactcggaaa tttgacatgc gatccgtacg tcaagacaaa
     6061 cggattatag tcgaagtgcc acaaacggtc aacgatccgg gcgagccgat attaatccca
     6121 gctgccccaa cacctccagc gaggccacca cgacgacggt atccccggcg cccagcgcct
     6181 cgtccgaccg tgggtgaaga tgcttttcgt tggggcggcc atgcagaacg atagtcaggt
     6241 cgtgctggcg ctccacttgc tcgatcgcct tcccggccag cggagagcct ggctccaccg
     6301 ccacgcgcgc cacgtgcagc aactggtcgt cgacgtagaa ggaatactcg acgtgcgccc
     6361 gcgccgctgc cgctgcgaag gccggcgcgg ccaggctggc cgtgctgaac acggtgtgaa
     6421 tgccgaagcc gcgctcgacc ctggccgcca ggtcggcgtc gaacatgcgc agaacgactt
     6481 tgacgccggg ccgctgctcg cgcgcgtcca gcgcgacgtc gaggttggtg agatcgtctt
     6541 gcgtgcagca cacgatcgac gtggcgcgct cgatgccggc cgacttgagc gcgtcgccgt
     6601 ggcgcgcgtc ggcgatgatc acgggcacgc ccagcttgcg caccagcgag acgaacggct
     6661 tgtccggctc gacctccacg gcgaccacat cctgaccgaa atcgagcaac tgctgcacga
     6721 tgcggtagcc gagcttgccc aggccgacga cgatgacgtg attcgagaat gtcgaagcca
     6781 atgccgcctc ccacgcgctg ccgcgcgccg acttgttgaa cagcagcacg ccgagattcg
     6841 ccacgccgct gcccagcagt gccaggccga tgaccggcat ggcaaagaag aagatctcca
     6901 ggtaccacgg ctcgggaaac tcggcgccgg cctggagaaa gatcatcgac agcatcaggt
     6961 agatggattc ggggatggag ggcgcgtcga cggcggggtc ttgcgcatgc gcggccaacg
     7021 cgagcacgcg atacaacacc gccgcgccga cgagaacgac gaggaaaacg aaaatcgggc
     7081 cgcgaaactc gcgcagcacc acccacgtat cgcgcaagcc ggcgcggaca cgccgccggg
     7141 tggaatgcgt gcgaagccgc gaggccatac cgtttgcagc gtcgctcgat cagccgccga
     7201 ctgccccgat gatcagggca aatccgacga tgcccagcgc gagcatgccg gcgatcaagg
     7261 cgactgtctg ccagccgccg gggattgacg cgagcgacaa ctgcgggcgc gatcggggtg
     7321 tcctgagcgg ctgaggcggc cgggggagcg gcttcgacag gcgcgacctg agcgccgggg
     7381 ccgcccggcc agccgccagc gtcgggtcgg gctgcagagt cgtgccgcgc tgcgacacga
     7441 ctttcggctg cgccggcgcg gccgggttgg cctgaatggc cgccgccgcg cgctgaacga
     7501 tattggcagg ctgtgaagcc gggagcactt cggcgtgcgc cggccgaacc ggcgcagccg
     7561 gcgacgcttc cgcctcggcc atcgccggct tggacgcggg aagcggctgc gtcgtgtcgc
     7621 gcaccggcgc ggccgcccca gccgacgcag cagccgcagc ggccgcggac gctttctgcg
     7681 cttcgcgcag caatcgatcg tattcctcga gcgcgatcag caccgccacc ggcttgttcg
     7741 gatcgccgat gagcaccggc gcgttgctgc gccgcatgtt gtcgatgacg agctgcgtct
     7801 cggtcgccag ttcgtgaacg gaaacgacga tcttcataat aggcacctca ccgtacgcct
     7861 ggtagcctgc aacggccggc acgcaagcgg gcgcggacgg caggcacaga cacactttgg
     7921 ctcacggagg atggcacaca ttatatacga gacgggccag agggcaaatg gccctcaccc
     7981 ccccggcccc tctccggccc tcacccccca gcccctctcc agccctcacc ccccagcctc
     8041 tttccagccc tcacccccca gccccctctc ccgaagttga aaagcgcact tcgggagagg
     8101 gggagatggg agtggggtca cacgaacgcc tcgcgccgga cagtcacggc atccagtcgc
     8161 gtcaggttca ccgctacccc caagccgggg ctgatcggca cgctgatggt cgaatccgag
     8221 ttgagcgtga aaggcggatc aacgatgtct tcgtgaaaat aacgatcgct cgcgctgacg
     8281 tcgcccggca gcgtaaagtt cggcagcgag gcgagtgcga cgttgccggc gcgcccgatg
     8341 cccgtttcca acattccgcc gcaccacact ggcacaccgc gcgactggca caggtcatgg
     8401 atgcgccggc tttcagtgaa cccgctcacg cgcccggctt tgatgttgat cactctcgct
     8461 gcgccaagct cgatcgcgag gcgggcgtgc cccagtgagt gaatcgattc gtcgaggcag
     8521 atcggcgtgc gcaggcgcgg ctggagcttg gaatgctcgt agaggtcgtc ccagcccagc
     8581 ggctgctcga tcagcagcag cttgtgctcg tccatggcct cgaacaacgg cgcgtcggcc
     8641 agcgtgtagg ccgagttcgc gtcgacttgg agaaggatgt cgggatgctt cgcccggatg
     8701 cgtttcaccg cttccaggtc gtaccccggc ttgatcttga tcttgatgcg ccggtagccc
     8761 tgcgccagat acgcgtcgac cgtgtcgacg agggcctcgt aggaaggctg aatgccgacg
     8821 ctcacgccca ccgacacgcg ctcgcgcgtc ccgcccagcg cacgagatag aggaataccc
     8881 tcgagctgcg cagtcaggtc ccacaacgcc gcttccagcc cggctcgcgc cagcggatgg
     8941 ccgcgcaact gctgcccggc gtcgaccatg tcatcgatcg acagcggccg accgagcacc
     9001 tgtggcacga gaaagtcgcg caggatgtgc cacgctgtga cggtcgtctc gtacgagtag
     9061 aggtgccagt ccaggcgcga cgcgccgcct gtccccgtcg tggcgcggaa ggggtcggca
     9121 gcgaagccgc gcgaagcgac gcattcgccc cagccggtca aaccaccgcc gtggaccgac
     9181 acgatgatgc actcgcgctc atgctccgtg ccgaaggagg tccgaaacgg cgctttgagc
     9241 tgcatgcgca ccagacggag ctcgatgcgt tcgatgtgca gatcggaggt tggaggttgg
     9301 aggttggaca ttggaagttg gacgttggag gttggaggtt ggaggttaga gactggactt
     9361 tggattttgg agtttgaagt ttggagtttg gatcggatca atctgccggc gatgcctcgc
     9421 tcgattcggt gcgggtgagc acgtagaagg cgcgcggcgt gacgtcgcgc tcgtacagca
     9481 tgtcgatcac ggtgaagccc gccgcgaagg ccggctggaa gattgcgcgc gtatgctcgc
     9541 gccaggcgag tgccagttgc gtgtcgaggc gcttcacgtc ctggaacagg gcggggaact
     9601 cgaccagcgc gcgatcgtcg tggagcggct cgatcgcatc gggcggcacc ggaaggccgc
     9661 gctcgtcaaa tgtcgcctca ttcaggattc tcgtcccagc gcgctgatag agagcacgcg
     9721 tcagcggcgg gcgccgccgt gacaggcgcg tctcgacgcg cttgctcgca atccaccaat
     9781 cgacctggaa gcgatcggtc ggcagccccc ggttcagatc gtcccgcaac tcaccgtaca
     9841 gattggggat gtacgtatcg cacacggcgc caagcttggc gatgttgagg ttggcgttcc
     9901 ggctttcgag tggatcgtac gtccaggtga tcaggcgcag cgcctggctg cgcacggctt
     9961 cgcgctgggc gagcttgagc gcgtaaccca ccccccggct ttgccactcc ggcagcacgc
    10021 cgagctggtg cgagcaatgc ttgagcttca cctgcgcggg cgcttccggg gcatagagcc
    10081 cttcgcgcgt gccgatgaag ccgaagacgc agccgaccat gtgctcgcgc ccaccgctca
    10141 cgacaaacgc gccgagcacc acgccgccgt tcttggcgaa cgtcagcagc acgtgcgacg
    10201 gcgtgacgtc gagatcgtcc aggcgccaca cgacgcgctg cagctcctcg gcctggcggc
    10261 attcgtcaat cgtcttgaga ggccggatgg agaaactgtc tgtcatgact gtgccaatca
    10321 gttccctgct tccttcggca ccccggccgg gaactcgagg aactgaagga actccacgtt
    10381 cactcgcgcg ccatgagata gcggacgaac atgcgccgcc acggccgtgg ccgtttgaga
    10441 tagtcgagcg agtcggacat gcgcgccgcc tggaagccga aatggaacgg gacggcgtcc
    10501 agcgcggtgg tgcggttgac cgcgagcaga tcgacggccg aattgctgaa cggcgggcgg
    10561 ccgagcaggc gcgaagacaa cctgacgagg gagtgggcga ctctcgggtg cagatggaat
    10621 cggcgccgct tcacgcccgc agcgttcatg atctcggcca ccgcctcgtc gaacgtcatg
    10681 acctgtggcc cgccgatgtc gatcgtctgc ccgatctttt tggagtcact caagcaggcg
    10741 atcaggcacg tcaccatgtc gtgcacgcac agcggctgca ggcgcgaccg cccgtcgccc
    10801 gggatgggaa agacgaacgg caaggcgtgc atgcccatcg ccataggcat cgtccatttg
    10861 tcatcctcgc cataaaccgc cgaggcgcgc agtgtggtat aggccaaccc gctcgtgcgg
    10921 atcagctcct cggcctgccc tttgcaccgt agatacggat aggccgaatg gggatcggcc
    10981 ccgatcgggc tgacgaacac aatgtgctcg acgccgactt cacgcgcggc ctcgatcaca
    11041 ttctgcgtcc cgccgacgtt gaccgattcg atggtgcgtc cgctcgaata gggctgccag
    11101 gtggcggcca ggtgcacgat ggacgtcacg ctgtgcatcg ccaggcgcag tgtgggcaca
    11161 tcgtcgacgt cgcccgacac gacgcgcacc gagacacccg gagcaaactg ccgcacgcgg
    11221 cgcgccggcc ggaccaggca cacgacgtct tcgcgcccgg ccaagcgcgc gacaagctgc
    11281 cggccaacca ggccggttgc acccgtaatc aggatcatgt ttgcggctca ggggatgagt
    11341 gcccgagatt atagcacgct tttcaaacgc gctgtcaccg gtcattacgc acctggtaag
    11401 gggtcaagcc agccgcagct cgccgatcac cgccaggccc accgcgacga gggcgagact
    11461 tccggcataa actgccaact gcgccttttg ccgctcgcgg tcggggcgat cctggaagcg
    11521 gcggccgagg tgtgccacga acaccgccgg gagcatgatg atgggatgaa tccaccgcac
    11581 cgaatcggac tgtgccagca gcatcagcgc caggatcgct ccgacggcgg cctgcaagtc
    11641 gagcgcgccg gtgtagcccc ggctcaacac ttcgtcgacg cgcatgaacg tcgcgttgcg
    11701 tgtgcccagg ctgacgagtg cccgaagtat gcccagcgcg cccagcgtca aaatgagcaa
    11761 ccgcaaatag gtatgggcgg tcaggataat atccatcgac ggttccgctc cgtaaggcag
    11821 ggtcggatcg aaatatactt gcatttgcga gatatgtaaa ctacgtgcga tgcacgtagc
    11881 gccgtatctt ttaggtcggg tctgtgttat atgcccttgt aaccgacctc tgccggctgc
    11941 agatcgtgta taatcacggg atacaccatc cgcaaggggg acaacgtgag acgagtggta
    12001 gtcaccggca tgggcgcgtt aacgcccgtg ggcaacgacg tcgatacgat gtgggcgagc
    12061 ctcgtggcgg gcaagtcggg catcgcgccg atcacgcatt tcgacccgtc ggcattcgag
    12121 acgcgctttg cgggtgaagt gaaggatttc gatccggtcg cgctgttggg ccgcaaggag
    12181 gccaggcaca tggatcgctt cacccagttt gcgatggccg cgaccggcca ggcgctcgcg
    12241 gattccggcc tgaagatcac gcccgataac acactgcgcg tggccgtgat catcggatca
    12301 ggcgtcggcg gcatcagcac gatcgtgggc gaagtcaacg tcctgcgcga cagaggcgcg
    12361 aatcgcgtca gcccgttcct ggtgccgatg atgctctcgg atacggcgcc gggcacggtg
    12421 gccatcgcct acggtatccg tgggccgaac ctgtccattg tgacggcgtg cgcgaccggc
    12481 acgaacgtga tcggcgaagc ggccgagatg attcgccacg ggcgtgtcga cgcggccatt
    12541 gccggcagcg ccgaggcggg gatcatcccg ctggcgatgg cgggcttcaa ctcgatgggg
    12601 gccttgtcca gacgcaacga ccaacctgaa aaggccagcc gccccttcga caagaatcgc
    12661 gacggcttcg tcgccagcga gggcgcgggc gtgctgatcc tggaagcgct cgagcacgcg
    12721 ctggagcgcg gcgcctcgat ctacgccgaa gtgatcggct acggctccag cgccgacgcg
    12781 tttcacatca ccgcgccgga agagaacggg gcgggcgcgg cggcggccat gcgcatggcg
    12841 ctcgatcagg ccggtttgcc gccgtccgac atcgactacc tcaacgcgca cggcacctcg
    12901 acgctgctca acgataagag cgaaacggcg gcgatcaaga cggtgttcgg cgaggcggca
    12961 tacgatgtgt cgatctcctc gaccaagtcg atgaccgggc atctactcgg cgcagccgga
    13021 tcggtcgagg cgatcgcgtg catcaaggcg ctgcaagagg gcattatccc gccgaccatc
    13081 aactacgaaa cgcctgatcc cgaatgcgac ctggactacg tgccgaatac ggcccgccgg
    13141 aaagtcgtgc gccgggccat gtcgaactcg ttcgggttcg gcgggcacaa tgcctgcctc
    13201 atcctcgctt ccgtcgacaa ccgcggcgcc gatccgactt cgaacgtctc gtgagcagca
    13261 ggtagaggtg tgccatggcc gagttcagga acggacgcca cgcccacatt gtcggctggg
    13321 gcaagtacgt cccgagcaga gtggtcacca atgcggacct ctcgcgcacg gtcgacacga
    13381 gcgacgagtg gattgtcaca cgcaccggca tccgcgagcg gcgcgtggcc ggcccgaaag
    13441 acacgtccgc ctcgatggga ttggcggcgg ccagggaggc gctggagagg gcaggagttc
    13501 gaccgcacga gatcgatttg atcgtcgtgg cgactgccac gccggaatac gcgtttccgg
    13561 cgacagcctg cgtaatccag gatgcgctgg gtgcggtgaa ctccggtgcg ttcgatctct
    13621 cggcgggctg caccgggttc atgtacgcgc tggcgctggc cacgcagtcc attcagtcgg
    13681 gcgcgcacaa catcgtgttg gtgatcggca cggagacgct tagccggatc atggattgga
    13741 acgaccgggc gacgtgcgtc ctgttcggcg acggcgcggg ggccatcgtc ttgcgcgcga
    13801 gcggccagaa cggtggcgtg atgtccacgc tatgccggtc ggacggctcc ggcggtgagc
    13861 tgctgatcat ccctggcggc ggcagcaagc atccggtttc gcaccagacg atcgatcagg
    13921 ggctgcattt catcaggatg aatggccgtg aagtcttccg cttcgcggcg cgcatcatgg
    13981 acaagtccgc gcgcgaggtg gcgtccaagg ccggcctcac gatggacgac gtacagctcg
    14041 tcatcccgca tcaggcgaac ctgcgcatca tccaggctgc cgcgcgcgcg ctcaaattgc
    14101 ccgaggagcg cttcttcgtc aatctgcaat actacggcaa tacgtcgagc gcgagcatcc
    14161 cgatcgccat ctgcgaggcc gtcgagcagg gcaagctgaa acgcggcgac cactgcgtgc
    14221 tggtgggctt cggagccgga ctgacctggg gggcagcggc catcaagtgg agcgggcccg
    14281 cgccgctgca cgagccaacg cgcctcgagc gcgtctacga gacactcctg tacgggctgg
    14341 ccggtattcg ttcgcggctg gtgcgggtgc tgcgccggat cgacagcgcg ctgttcggat
    14401 cgccgtcgcc tgggcgcatc gacaaagacg gccggcgaaa gaagtaagta gtagcgactt
    14461 cagtcgcttg ttaacgactg aagtcgtcac tacgctaaac gactaccttc gacagcgccg
    14521 cgtcgaggcg ctgcttgatg atgggatatg gcgccgcgcc gacaatgcga tcgatttcct
    14581 ggccgtcgcg cacgaacagc agcgtgggaa taccctgcac gccgtacttc atcgcgtatt
    14641 cggcattgtc gtcggtgttg accttcgcga taaggatctt tcccgcgtac tcgccggcca
    14701 gcttctccag cgacggcgcg accatgcggc acggcccgca ccagggcgcc cagaaatcga
    14761 ccagcacggt cagattgctc tgcagcacca ccttctcgaa cgctgcatcg ctcacatgaa
    14821 tcggttcgtt atgcatctcc atcctccaga atttgatacg actgagtaat cgctgccact
    14881 ccgctcaccg tcgtggacac tgagcagtac ttcttttcag acaaatggag ggcttgttga
    14941 acagccctgt cggtcaggcc cttgccgcgc aaaatatact cgacgtgaat cttgcgatac
    15001 gtccacggcg gatcggggtc ttgttcgccc gagattttga tgtccaggct gcgcaggtcg
    15061 atccgcttct tttgcagaat gccgacgacg tcgaccgccg agcacgcgcc cagcgccacc
    15121 agcagaagct cgctgggtct catgccgacg ccctccctgg cggtcgacat gacgaccgag
    15181 tgcttcgtgc tgtcgattcc tacgaatctt tgtccgccta tccactgaac gctgacttcg
    15241 cccataacta gactttctcc aacacactca ccaagctatc cagcacctct tgcggcgaga
    15301 cctgctcgcc ggatttgaac aggcaggctg cggcatgctc gcgcacgaca accaggctgg
    15361 cctgatgggc ggccgcgcgc actgccgcga gctgctggag cacctcccgg cagtcgcgat
    15421 cctcgtcgag catcttctgc acgccgcgca cctggccttc caggcgcttg agccgcttga
    15481 gcaggtctgt cttgacttcg ggtgatacgt tcatttcgcc ctctcttgaa ggcggcccga
    15541 cgggccgcta gggacggatc ctcagccacc gccggacggc gcgcacgcca gggcgctcgc
    15601 ggaggagcca ccccgcccca cggcggccat gagggagacg acgcgctttg cgtggcgcgc
    15661 gccgcactgc gggcactcca cgtcttcggg ccgggtggac atgcgcacga gcttctcaaa
    15721 ccgctcgtcg cagtcttcgc actggtactc gtagatcggc atatggggct ggacactccc
    15781 aagacaatat actatactgg ggtatagtat agcacggact ttcagattgt caagtccgtg
    15841 gcccggctgc atgggcgccc gctgaccctc ttgacaatct ggcttaatgc tatataatca
    15901 acttgattca ctgaacgaat aaactggagc tatcttcttg tcccaattgc gcaaaggcaa
    15961 ccgcgatctg atcaaggaaa tcaatcgcaa cctagtgctc aacctcatca agagcaaagg
    16021 ccctatctcc cgcgttgaac ttgccaaact gtcagggtta agcccggcca ctatctcggg
    16081 cattgttgct gacttcacag ccagcgacct ggtacacgag atgggtgaag gggagtccag
    16141 gggtggccgc cgcccagtgc tattgcgtct caatcaccgg gcgggttttg tagtaggcgt
    16201 gaagttgatg gataacgcga tcgccagtgt cctcactaac ctggatgccc aagtgctcta
    16261 tcatcgcgtt gttcctctga aggtttctcc cctccaccca acctcgggtg acgagcgtac
    16321 ctcttcaaca tcagggactg agacgccctc cacggtcggc gtggcaaagg aatccatcct
    16381 tagcgcgatc atccaaacca ttgaggtcac aattgctgag tctgaggtgg accgccaacg
    16441 ggtgctgggc attggcatcg ggatggcggg tgtggttgat ggggagacag ggatttgccg
    16501 ctactctcct tttttcggct ggcgcgatgt gcagatcgcc gaacctatcg ccgctcattt
    16561 caaactaccc gtgtacctgg agaatgatgt caacacgcta accattgccg agcagtggtt
    16621 tggccatggt cacggtgtcg accacttcgc cgtgatcacg accggtcgag gcgtaggcac
    16681 tggtatcgta gtcaacggcc agttctatcg aggtgctgtg gggggtgccg gggagttggg
    16741 acacatcacg cttgtcgagg atggcccgct atgcgattgc ggcaagcgcg gctgcctgga
    16801 agcgctggct tcagacccgg cggtgattag ccaagccaga ctcgccgtcg ccgcaggtaa
    16861 gccgaccata ttggttgagg caaagccgtt gacgctcgag acgatcatcg ccgctgccga
    16921 aggtggcgac gaactcgctc gacagctgct ggccgattca gggcaatggt taggcatcgg
    16981 tatcgctacc cttgccaaca tcctcaatcc gaagttggtg atcgtcagtg gggagggtgt
    17041 gcgcgccggt gagtggcgct tcggcccaat gcggaaggca cttcaagatc acactttcaa
    17101 cgacttggcc gatgggatgg agattattat cgaagcgacg ggcgatgaaa cgtgggcacg
    17161 tggggcggcg tgcgtcgtgt tgggggagat gttcaagtcg ccggtacaac gggaaaaggc
    17221 agtggatttg atggcatgaa gcggacggcg atcggctgaa gaccgatttt attttcgccg
    17281 gacttacttc gtttagagaa cgaagctaat acaaaaaaca ccactcgcgc cgtttacatc
    17341 aggctctcac cccctccagg cggcgggcga gggtactgta accgtaatgc tggcagccca
    17401 acgtccagca gattcaactc atcaggagaa aactaccatg agacagcata agtccttcgc
    17461 atggctatcc aagctcctca tcgttgctct gcttctctcc tcgattgaat tcgcgctccc
    17521 cactccggtc gccttcgcct tgagcggcgt ttaccacaac ccttacggcc aggatgacct
    17581 atactcaacc gagccaactg aacggagtcc tcgcgatccc caggccggcc agacggtcta
    17641 catcaagatc actacctggc cgatcgaatg gggccaggcg gtctggctca cctggaccaa
    17701 gaacggcgtg ccccagtccg atgtaggcgc gcagtggaaa tacaacagcg gcaacaacag
    17761 ctactgggaa gccgcgttcg gcccctttca aaaaggtgac gacatcacct acaccgtcca
    17821 cgccaacgtg aacggctgct gtgaacgaac caccggcccg ttcagcttta ccgtcaccga
    17881 ctggagcacg gtgacgaacg tcaccggcca caccaacaac accaactacg tgcggtttac
    17941 attcggcgac tccggtggca acttcacacc ggccatgcgt ctggctttcg tcagcgatca
    18001 ggtctttcgg gtgcaattct cacccaccgg gcaagggctc aatatcaccg gcctgaacaa
    18061 ctacaccgtt tccgaagatg cgacttacat caccgtcacc accagcgcgc tgcagctgaa
    18121 ggttcagaag aatccctacc gcctcttcgt ctataaagct gacggccaaa ccctgatcgc
    18181 caaagagtat gatccctcag tcttccgcat cttcgagtgg ttgaccaatg gcgacacggc
    18241 ggtggccaaa atcggcaccc acttctacac gccgaccacc gagaagttct ggggtctcgg
    18301 tgagcgctac aacttgctga accagcgcgg caaagatgtc aacatctaca ccgtcgacca
    18361 gtatctggat cacggtgagc gcacttacct gcccgttccc ttcttcatca acagcgccaa
    18421 ctacggcgtt tacctgaaca cgacctacta ctccgtcttc aacatcgcca cctacctgag
    18481 cgacatgtat ggcttcactc agaacacgag cggctcgctc agttcgacgc tcgattacta
    18541 tttcatctac ggttccacgc cgaacgagat tatccagcgt tacaccgacg tcaccggcaa
    18601 gcccaaattg cctcccaaat gggctttcgg cccgtggatg atggctaacg agtgggacaa
    18661 acaatccgag gctcaaaccc agatcgacaa caccgacact tacgacattc cggccacggt
    18721 actggcgatt gaggcctgga gcgacgaggc aaccttctac atctggaacg atgctcaata
    18781 cacccctcaa ccggggagct actgcttcac ctacaacgac ttcaccttcc cggtcggtgg
    18841 caaatggcca aatccgcgtc agttggtgca gaacgcccac gacaaaggca tcaaagtctt
    18901 gctctggcag gcgccggtcg aaaagtggat gagcagtcca tatacccaga aggacaacga
    18961 tgaatcttat atgctccagc agggatacgc cgtgggcgac gggtccggcg gctcgtatcg
    19021 catcccgggc ggacgctggt tcgcgaacag cctcgtgcct gactttacca actcggctgc
    19081 caccgactgg tggaccaact gcaagcgcgg ctacctgttc aacatgaacg gggtgagcat
    19141 cgacgggttc aagaccgatg gcggcgagta tatctacggg cgcaatctca ccttcgccaa
    19201 cggcaagaag ggcgacgaga tgcgcaacgc ctaccccgga tcttacatcg gcgcctttca
    19261 tgacttcgtg cagacgaaga ctatcagcaa cggcatcctg ctgagtcggg ccggtgcggc
    19321 tggtgctcaa gcctaccccg gctactggtc cggtgacgag cgctcgacat tcagcgcctt
    19381 ccaggactcc atccgcgcgg ggttaagcgc cggcatgtcc ggtgtgccat tctggggctg
    19441 ggacctggcc ggattcagcg gcgatatccc caccaaggaa ctctacctgc gcagtgcggc
    19501 gatggcagct ttcacgccga tcatggagta ccactccgag accagcggtg acccaaaccc
    19561 atctcgcgca cgatcgccgt ggaacatgga agcgcgcctg agtgactcac aagcgcgcac
    19621 cggcttcgcc aaattcgcga acatccacat gaacctggtt ccgtacagtt acagcgaagc
    19681 caagcagacc agccttaccg gtgtcccgat gatgcgcgcc atgccgctgg agtttcccac
    19741 cgatgccacg gctgtcaatc aagtcttcca gtatctgtat ggcagccagt tgctggtagc
    19801 gccggtcgag acgcaaggcg cgacaaccaa gtcgatctac ctgcctcggg gtgagtgggt
    19861 ggatctatgg aacggtggac agcacagcgg cccgatgacg gtcacctatg gcgtcagcaa
    19921 cctggaaacc atccccgtct tcgtgcgagg cggagccatt ctgccgctca acctggacgc
    19981 caattacacc ttaggcggct acgtcggtaa cacggttgac aactacacgc ggctcacctt
    20041 tgctatgtat ccctttggca gctcgagctt cccgtggttt gatgacccca tcaacactgt
    20101 caagaccatc agtgtcaccg agaactatct gaatcagcag gtgatggtca gtgtaccacc
    20161 ggtgagccag acggtgactc tgttggtgaa caccactgtg ccgatcagcg tgacggtgga
    20221 cggcaacgca ctcacccagt acaccaactt tgccagcttc caggtcgccg cgaccggttg
    20281 gtactacaac actcagaacc aggatgtcta cgtcaagatc ggctccagcg cggcgactcg
    20341 cagcgtgacc ctaaatggcg tgaacaaggc cgcctacgaa gcggagtttg aagcgctcac
    20401 caatgtcagc acgaacaccg accaccccgg ctacacaggc atcggcttcg tggacggctt
    20461 cgagacccag aacgacgccg tcaccttcga cgggattgaa gtctccaagg gcgcgacatt
    20521 tgaccttcgc tttcgctaca gcgccgccaa cgcgggcgga gccacgcgca ccatctacgt
    20581 caatggccag aaggtcgggc gcatcacctt cccgcagacg acgaactggg acacctgggc
    20641 cacggcctcg atcagcggca acctgtatca agggcgcacc aacacagtca agatcgtata
    20701 cgaagccggc gacagcggcg cgatgaacct cgataacctg ctgctcgtgg cgctgccgat
    20761 ccgcccggcg cgcgagtact atcaggaaga ggccatgcta gccaacggct atgccttcgc
    20821 ccagctcgat gcgcgcggtt ctctgtacga tctgatgtac cccttcggca tctacaccgg
    20881 cgtcgtcgtg gatggcgccg atgcctccgg cacacagggc gtgcaggtga atatctgggg
    20941 agccttgggt ggcgtggagg tagacggcaa gacccactgg ctcaatcacg gcgaccaatg
    21001 gaccttcagc cagcagtaca tcactgacac agctgtgctc aagacggtgg cgacacacaa
    21061 cacggccagc gtgcgcgtga cccagtatga cttcatcccg gacggcatca ccttccccaa
    21121 cacgaccggc ggccagccga tccgtggcat gtacctcaag cggctggtgg tggaaaacct
    21181 ggccggcacg gcgcgggaca tgggcctgct gtactatgcc gacctgaaca tcaacggcag
    21241 cacagcgcaa gatagcgtca gctacaagac ggccgagaag gctctctact tctacgatgg
    21301 cggcgatagc gggagtggtc gcacgcgcac actggctttt ggcctgcgcc tcaagtcccc
    21361 tggctcggtg atcaccgaaa cctatcagct ctacagcggc gacgcggccg gctacctcaa
    21421 gcaagtggcg agtatccctg ctgccggtag ccaggagttc gatgtgctgc tggtgggcgc
    21481 cacctctacg acgacgggcg ccgatctgta cagcagtgac atcgtgccgg ccatcaactg
    21541 gttcaacagc cagaccatca atagcgtgca gacaacgacc gagaatacct ggacgaccct
    21601 gatcaacggc gctaccgtgc tggacacgcc tgacccggtt tacgatgcca actatcggcg
    21661 cagcctgatc acgacgatcc ttactatcaa taaagacacc ggcgccgtag cggccggctt
    21721 ccacaacggc gcttacgcct acaactggcc gcgcgacagc gtttacgcgg cgatgacgct
    21781 tgaccgggcc ggtattcacg atctgccggc caagcaatac gactggctgt ggaacgtcgc
    21841 tgaacgggat acgtccagca atgacatcgg cggtgatggt gtcttctatc gcttctggta
    21901 tcagaagtat acgatggatg gcgtacgcgc ctgggagcag ccgcaggtag acgagacggc
    21961 ggtcattccg ttcggcgcca agctgcactg ggatatgact ggcgacacga cattccgttc
    22021 gacctactat ccgatggtca aagaggcggc cctggtctcg tcggagaata acccgcaccc
    22081 cggtctggac tacagcgaaa gcatgcatct catgttctcg tacaacatct gggaggacat
    22141 gtggggcatg ttgctatact ccaatgccaa cgtcgtcgcc gggctggacg cggccgcggt
    22201 tctggccggc ctggaaggtc agaccagcga tcaaaacacc ttcatcagcc ggcgcgacga
    22261 catcaagaac accggaatcc tgcagtctgt gtcgtcctcc aacaccacca gccctggtct
    22321 gtactcctcg gactacaatc gtttctacat gaccaaggat ttgaagaagt ggtataccga
    22381 tcccagcaac atctggaatg ccatcacgac cgacgtgtcc atgctgggca ttgtggtacc
    22441 cttcggcatc ctgtctgccg atcacacctg ggtagtgaac accgtcaacg aattggagcc
    22501 ggccctgacc gattcggccg aaacgggcgc tgccatcggc ggggtggtgc gttaccgcca
    22561 ggatcaggtg agccgctatg gcagcacgta cgccaattac ggcgacactt actatgacgg
    22621 cgggccgtgg atggtcgcca ccacctggct cagccagtac tacctggaac gggccaatac
    22681 g
//
LOCUS       DBNHXB010000198         3526 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3399962, whole genome shotgun sequence.
ACCESSION   DBNHXB010000198 DBNHXB010000000
VERSION     DBNHXB010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3399962"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..647
                     /gene="tsaE"
                     /locus_tag="AC162G_08085"
     CDS             138..647
                     /gene="tsaE"
                     /locus_tag="AC162G_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR5075809.1"
                     /translation="MSPILAPDVFEFVSGSPAQTMRIGERLGSLLQPGHVVCLAGALG
                     TGKTCMAQGIGAGWGAAGEMTSPTFTLVHELRRVSDGATLYHIDLYRIESEDEARLLG
                     LSDLFERDAVCLVEWPERAGDVLPDACLRVELITLDETRRRLTFSAEGERHLHLLSEL
                     KRAAFGVGE"
     gene            744..1409
                     /gene="tsaB"
                     /locus_tag="AC162G_08090"
     CDS             744..1409
                     /gene="tsaB"
                     /locus_tag="AC162G_08090"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR5075810.1"
                     /translation="MLLAIDTATQRASLALHDGGCVRAEMTWEMAGHHTVELMPRILW
                     MLEQLGAKACDLTAVAVAIGPGSFTGLRIGLAMAKGLSLANSIPVVGIPTLDVVAVAQ
                     PVRKEPLVAVLHAGRGKLAAMRYRRTRGEWRAQGDVAVTTTGRIGDDWDRPTLLCGEL
                     DAAERASIVARLGDRVILADPAHSLRRAGFLAELGWRRVQSGAVDDLDALKPIYLPTA
                     SVT"
     gene            1558..2046
                     /locus_tag="AC162G_08095"
     CDS             1558..2046
                     /locus_tag="AC162G_08095"
                     /inference="COORDINATES: protein motif:HMM:NF020419.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HIR5075811.1"
                     /translation="MDQTNLLERLTALLAEHQIRYCVIGGQAVNAYVEPLVSLDLDLV
                     VALDQLGQAESILASSFKLERFPHSLNVSLPGSDVRVQIQTDPRYAAFVEHASPREVL
                     GLAMPVASLDDVLRGKVWAIQDPTRRASKRQKDLADIARLLDAYPALRDQVPGDVRAR
                     LL"
     gene            2096..2713
                     /gene="rimI"
                     /locus_tag="AC162G_08100"
     CDS             2096..2713
                     /gene="rimI"
                     /locus_tag="AC162G_08100"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR5075812.1"
                     /translation="MTESPVSFEKMQLKDVPAVMAIEQRSFTLPWSANTYRHEILENQ
                     SAHYYVLRHRNGAPGRRADWLSRLARREAGAPIVGYGGFWCIVDEAHISTIAVDVGWR
                     GRGLGELLLASMIEQAIVLNSVKVTLEVRESNRVAQNLYRKYGFVVASTRPRYYQDNG
                     ENAFLMALEGVSGEAYRRALRDLTGLLQARLASADGEPIRARADR"
     gene            2812..3426
                     /locus_tag="AC162G_08105"
     CDS             2812..3426
                     /locus_tag="AC162G_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HIR5075813.1"
                     /translation="MSEHDSLDRIAAEVSACTKCILHKSRVKAVPGEGLAHADIMFVG
                     EGPGFHENQQGRPFVGAAGQFLEELLASIGLKREQVFITNVVKCRPPGNRDPLPEEIE
                     ACKQYLDRQIALIKPKVIVTLGRYSMARAFPNEKISRIHGQPKKVEGIVYVPMFHPAA
                     ALHQASLRKTIEEDFKKLPAILAEIETLRDEEPPSPQAQQLSLF"
BASE COUNT          665 a         1050 c         1150 g          661 t
ORIGIN      
        1 gtaactcctc caatgaccaa ttaccaatta ccacctacta agtcgcaatg acgcctgagt
       61 agtaaccaga gatgcacgcc ttgcgcgtct caagtgcggt tgtataatcg cctgcgacgc
      121 gcgcatcccg gagtggaatg tcaccgatat tagcgccaga cgttttcgag ttcgtgagtg
      181 gaagcccggc gcagacgatg cgcatcggcg agcgattggg cagcctgctc cagccgggcc
      241 acgtcgtctg tctggccggc gcgttaggta caggcaagac gtgcatggcg caagggatcg
      301 gcgcgggatg gggcgcggcc ggcgagatga ccagcccgac cttcacgctg gttcacgagc
      361 tgcgacgcgt gtcggatggc gcgaccttgt atcacatcga cttgtaccgt atcgaatccg
      421 aggatgaggc gcgcctgctg ggactgagcg acctgttcga gagagacgcc gtgtgcctgg
      481 tcgagtggcc cgaacgggca ggcgacgtcc tgcccgacgc gtgtctgcgc gtcgagctaa
      541 tcacgctcga cgaaacccgg cgccgcctga ccttcagcgc cgaaggcgag cggcacttgc
      601 atttgctgag cgagttgaag cgcgcggcgt ttggcgtcgg cgaatgagcg acgagtgaaa
      661 agcgatacgc aatatgcgat atgcgatgag cgatacgcga tacgcgatat gcgatacgcg
      721 atatgcgata agcgatacgc gatatgctgc tggcgattga tacggcgacg cagcgggcca
      781 gcctggcgct tcacgacggc gggtgcgtgc gggccgagat gacgtgggag atggcgggcc
      841 accatacagt ggagttgatg ccgcgtatcc tttggatgct ggaacagctc ggcgcgaaag
      901 cgtgcgatct caccgcagtg gccgtggcga tcgggcctgg ctcgtttacc ggcttgcgca
      961 tcggactggc gatggccaag ggcctgtcgc tggcaaactc cattcctgtc gtgggtattc
     1021 ccacgctgga cgtcgtggcg gttgcgcagc cggtgcgcaa ggagccgctc gtcgccgtgc
     1081 tgcacgcggg ccgtggcaag ctggccgcca tgcgttaccg gcgcacgcga ggcgagtggc
     1141 gcgcacaggg ggacgtggcc gtgacgacga ccggccgcat cggcgacgat tgggataggc
     1201 cgacgctgct gtgcggcgag ctggacgccg ccgagcgcgc ttcgatcgtg gcgcgcctgg
     1261 gcgaccgcgt gatcctggcc gatccggcgc attcactgcg gcgggcgggc ttcctggccg
     1321 agctgggctg gcggcgcgtg caatccggcg ctgtcgacga tctcgacgcg ctcaagccga
     1381 tttatcttcc gaccgccagc gtaacctaag agcataaaac ccgtagagcg ggcatcccta
     1441 tgcccgctgc gccggcgtaa cctaacgtga ccgtatgatc cgaatccgtc cattccaatg
     1501 atccgtgttt agtgaccgtg tgacatgcgc gctttcacct tttggaaggt aatttccatg
     1561 gatcagacca acctgctcga acgcttgacg gcgctgttgg cggagcacca gattcgatat
     1621 tgtgtgatcg gcggacaggc cgtcaacgcg tacgtggaac ccttagtcag ccttgacctg
     1681 gacttggtcg tagctctcga tcaactcggc caggccgagt cgatactcgc aagctctttc
     1741 aagctcgagc gtttcccaca tagcctgaat gtctcgttgc ccggatccga tgtccgcgtg
     1801 caaatccaaa cggatcctcg ctacgccgct ttcgtcgaac atgcttcgcc acgagaggta
     1861 ttgggactgg cgatgccggt cgccagcctg gacgatgtgc tgcgcggcaa ggtgtgggcc
     1921 atacaggacc cgactcggcg ggcgagcaag cgacaaaaag acctggccga cattgcgcgc
     1981 ctgctggacg cctatcccgc cttgcgcgac caggtgccgg gggatgtccg ggcgcgactg
     2041 ctctaacgag ctggggtccg tgtgtaatcc gtgcaatccg tgtggagcaa tccgtgtgac
     2101 tgagagcccc gtctcgtttg agaaaatgca gttgaaagac gtgccggcgg tgatggccat
     2161 cgagcagcgc tcgttcacgc tgccgtggtc ggcgaatacc taccggcatg aaatccttga
     2221 gaatcagagc gcgcactact acgtgctgcg ccatcgcaac ggcgcgccgg gccggcgcgc
     2281 cgattggctg tcgcggctcg ctcgccgtga ggccggcgcg cccatcgtcg gctatggggg
     2341 gttctggtgc atcgtggacg aagcgcacat cagcaccatt gccgtcgacg tcggttggcg
     2401 aggacgcggg ctgggcgaac tcctgctggc gtcgatgatc gagcaggcca tcgtgctgaa
     2461 ttcggtgaag gtcacgttgg aagtgcgcga gagcaatcgg gtggcgcaga acctgtatcg
     2521 caagtatggc ttcgtcgtcg ccagcacgcg cccgcgttat tatcaggaca acggggagaa
     2581 cgcctttctg atggcgctgg aaggtgtgag cggcgaggct tatcgccgcg ccttgcgcga
     2641 cctgaccggc ttgctgcagg cgcggctcgc gagcgcagat ggcgagccga tacgcgcccg
     2701 agctgaccgg taacggaatt cgggtctctc ggtggtaccc ttgttgatca tccccggctg
     2761 tggtatattg cccctcgctc cgcgaatgcc tctgtcacga aaaggtgatg tgtgtctgag
     2821 cacgattcgc tcgatcgcat agccgccgaa gtttcagcct gcacgaagtg cattcttcac
     2881 aaatcgcgtg tgaaggccgt gccgggtgag ggcctggcgc acgccgacat catgttcgtc
     2941 ggcgaaggcc cgggctttca cgagaaccag cagggacggc ccttcgtcgg cgcggccggg
     3001 cagttcttgg aagagctgct cgccagcatc ggcctgaagc gcgagcaggt tttcatcacc
     3061 aacgtcgtca aatgcaggcc tccaggcaat cgcgatccgc tgcccgaaga gatcgaggcc
     3121 tgcaagcagt acctcgaccg gcagatcgca ctgatcaagc ccaaggtcat cgtcaccctg
     3181 ggccgttact cgatggcgcg cgccttcccg aacgagaaga tcagccggat tcacggccag
     3241 ccgaagaaag tcgagggcat cgtgtacgtg cccatgttcc atccggcggc ggcgctgcat
     3301 caggccagcc tgcgcaagac gatcgaagag gacttcaaga aactgccggc gatcctggcc
     3361 gagatcgaga ccttgcggga tgaggaaccg ccatcgccgc aggcgcagca actcagcctg
     3421 ttctgaccca ccgatcactg atcactgatt actgatcact gattactgat cactgattac
     3481 tgatcactga tcactgataa ctggtcactg actatggaag acgaaa
//
LOCUS       DBNHXB010000199         3395 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_715976, whole genome shotgun sequence.
ACCESSION   DBNHXB010000199 DBNHXB010000000
VERSION     DBNHXB010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_715976"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1846
                     /locus_tag="AC162G_08110"
     CDS             <1..1846
                     /locus_tag="AC162G_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR5075814.1"
                     /translation="GQELIEKDIKDHDLTRVVVAACSPHLHEGTFRNACRRAGLNPYL
                     CELVSIREQVSWVHTDKEAATDKSKAVLSGGVERVIHHEPLEPLHVPIHEATLVVGGG
                     IAGIQAALEIADAGYPVYLVEREPSIGGHMAQFDKTFPTLDCAACILTPRMVSAGSHP
                     NITLLTWSEVERVDGYVGNFTVAIRKKARYVNTELCTGCGICQEKCPKRIIDNVYEAG
                     LGYRKAIYTPFPQAVPKYPVIDRPNCTYFLKGTCKACEKFCPTNAIDFEQQDEHITLQ
                     VGNIILATGYDIFDARKVSQYGYGRLANVFTSLEFERLSNSAGPTNGNIVLRDGVTMP
                     KSVGIIHCVGSRDRNFNNYCSAICCMQSLKFAHLVHERTGATVYNFYIDMRTPAKAYD
                     EFYQRVLEEGTLFVRGKVAEVTDAARLPGEEGRLIIQVEDTLAGKQRRIPVDMVILSI
                     GLEPRHDADEVAKLFGISCSADGWFIEKHPKLDPVATMTEGIFIAGCSTGPKDIPATV
                     AQGAAASARVLGRIQQGEIALEPVRASVDQDKCSGCRICNDLCPFNAITFLEDRMVTE
                     INPALCQGCGTCVAACPAGAISGTGFSNEQVMAQIEGLLMLNLEMVPA"
     gene            1916..2440
                     /locus_tag="AC162G_08115"
     CDS             1916..2440
                     /locus_tag="AC162G_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR5075815.1"
                     /translation="MPKNDEQFEPVIIGFTCNWCSYRAADLAGTARVKYPPNIRLIRL
                     MCSGRLDPTFVLKALSSGADGVLITGCHPGECHYIEQNYKALRRYLLLRRTLTQMGIE
                     PERVKLVWASAAEGVRLAEEITRMVEEIRALGPLQWPSHPSSVISYQSSAVSGQSTDH
                     RSLITEHSPEEVPA"
     gene            2437..>3395
                     /locus_tag="AC162G_08120"
     CDS             2437..>3395
                     /locus_tag="AC162G_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR5075816.1"
                     /translation="MTAEANGKPKFAMYWAAACGGCEIAVLNIDEKILDVDANFDVVF
                     WPVAMDAKYKDVEAMPDGSITLTLFNGGIRNEENEEIAHLLRRKSKILVAFGSCACEG
                     CIPALANLSSPREIFDTVYGTISTDNPNGVRPQYTYPAPEGDIHIPAFYPVLQTLDQV
                     VPVDYYMPGCPPESHQIAAVVDLVIKALHGEATLPPPGSTIGAGGSTVCDECPRARNV
                     KTIKKFVRIQDVRADPSLCLLEQGIPCNGLATRSGCGALCPQAGAQCIGCYGPAEGVI
                     DYGARLITAFASVIDSKDPVEIERILDGIPDPAGQFYRFNLAKS"
BASE COUNT          642 a         1106 c         1090 g          557 t
ORIGIN      
        1 ggggcaggag ctgatcgaga aagacattaa agaccacgat ctgacccgcg tcgtggtcgc
       61 cgcctgctcg ccgcacctgc acgaagggac attccgcaac gcctgccggc gcgccggtct
      121 caacccgtac ctgtgcgagc tggtctcgat ccgcgagcag gtctcgtggg tgcacaccga
      181 caaagaggcc gcgaccgaca agtccaaagc cgttctgtcg ggcggcgtgg agcgcgtgat
      241 acaccacgag ccgctggagc cgctgcacgt cccgatccac gaggcgacgc tcgtcgtcgg
      301 cggcggcatc gccggcatcc aggcggcgct ggaaatcgcc gacgccggct acccggtgta
      361 cctggtcgag cgcgagccgt cgatcggcgg gcacatggcc cagttcgaca agaccttccc
      421 cacgctcgat tgcgcggcct gcatcctcac gccgcgcatg gtctcggcgg gatcgcatcc
      481 caacatcacg ctcctgacct ggagcgaggt agagcgcgtc gacggctacg tgggcaactt
      541 cacggtggcg atccgcaaga aggcacgcta cgtcaacacc gaactgtgca cgggctgcgg
      601 catctgccag gagaaatgcc ccaagcgcat catcgacaac gtgtacgagg ccggcctcgg
      661 ctaccgcaag gccatttaca cgccgttccc gcaggcggtg ccgaagtatc cggtcatcga
      721 ccggccgaac tgcacgtact ttctgaaggg gacgtgcaaa gcctgcgaga agttctgccc
      781 gacgaacgcg atcgacttcg agcagcagga tgagcacatc acgctgcagg tcggcaatat
      841 catcctggcc acgggctacg acatcttcga cgcgcgcaag gtgtcgcagt acggctacgg
      901 gcggctggcc aacgtgttca ccagcctcga gttcgagcgc ctcagcaact cggccgggcc
      961 gaccaacggc aacatcgtgc tgcgcgacgg ggtgacgatg cccaagtcgg tcggtatcat
     1021 ccactgcgtc ggcagccgcg atcggaactt caacaattac tgctcggcga tctgctgcat
     1081 gcagagcctg aagttcgccc acctggtgca cgagcgtacg ggcgcgacgg tctataactt
     1141 ctacatcgac atgcgcacgc cggccaaagc ctacgacgag ttctaccagc gggtgctgga
     1201 ggagggcacg ctgttcgtgc gcggcaaggt cgccgaagtc acggatgcgg cgcgcctgcc
     1261 cggcgaggaa ggccgcttga tcattcaggt cgaggacacg ctcgccggca agcagcggcg
     1321 cattcccgtc gacatggtca tcctctcgat cggcctcgag ccgcgccacg acgccgacga
     1381 ggtggccaag ctgttcggca tttcgtgcag cgccgacggc tggttcatcg agaagcatcc
     1441 gaagctcgac ccggtcgcca cgatgaccga aggcatcttc atcgccggat gctccaccgg
     1501 cccgaaagac atcccggcca ccgtcgcgca gggagcggcc gcctccgcgc gcgtgctggg
     1561 ccgcatccag cagggcgaga tcgcgctcga gccggtgcgc gcgagcgtcg atcaggacaa
     1621 atgctccggc tgccgcatct gcaacgatct gtgcccgttc aacgcgatca ccttcctgga
     1681 ggatcgcatg gtgaccgaga tcaatccggc gctgtgccag ggctgcggca cgtgcgtggc
     1741 cgcctgcccg gccggcgcga tcagcggcac cggcttcagc aacgagcagg tgatggcgca
     1801 gatcgaaggg ttgctgatgc tgaacttgga gatggttccg gcgtgaaacg tgaatcgtga
     1861 tgcgtgatgg ccgattacgt ttcacgcttc acgttttacg gactggagga acgcaatgcc
     1921 caagaacgac gaacagttcg agcctgtcat catcggcttc acgtgcaact ggtgcagtta
     1981 ccgcgccgcc gacctggccg gcaccgcgcg tgtgaagtat ccgcccaaca tccgcctgat
     2041 ccgcttgatg tgctcggggc ggttggatcc cactttcgtg ctcaaggcgc tgtccagcgg
     2101 ggcggacggc gtgttgatca ccggctgcca tcccggcgag tgccactaca tcgagcagaa
     2161 ctacaaggcg ctccgccgct acttgctgct gcggaggacg ctgacccaga tggggatcga
     2221 gcctgaacgg gtcaagctgg tgtgggccag cgccgccgag ggtgtcagac tcgccgagga
     2281 gatcacccgg atggtcgaag aaatacgcgc actcggcccg ttacagtggc ccagtcaccc
     2341 gtcatccgta atcagttatc agtcatcagc cgtcagtggc cagtctactg atcaccgatc
     2401 actgatcact gaacactccc ccgaggaggt gccggcatga ctgctgaggc aaacggcaaa
     2461 ccgaaattcg ccatgtactg ggccgcggcg tgcggcgggt gcgaaatcgc cgtcctgaac
     2521 atcgacgaga agatcctcga cgtcgacgcg aacttcgacg tcgtcttctg gccggtggcg
     2581 atggacgcca agtacaagga cgtcgaggcg atgccggacg gctcgatcac gttgacgctc
     2641 ttcaacggcg gaatccgcaa cgaggagaac gaggagatcg ctcacctcct gcggcgcaag
     2701 tcgaagatcc tggtcgcgtt cggctcgtgc gcgtgcgagg gctgcatccc cgcgctggcg
     2761 aacttgagca gcccgagaga gatcttcgac acggtgtacg gcacgatctc gaccgacaac
     2821 ccgaacggcg ttcgaccgca atacacctat cccgcgcccg agggtgacat tcacattcca
     2881 gcgttctacc cggtgctgca gacgcttgac caggtggtgc ccgtcgatta ctacatgccc
     2941 ggctgcccgc ctgagtcgca tcagatcgcg gccgtcgtcg acctggtcat caaggcgctg
     3001 cacggcgaag cgacgctgcc gccgccggga tcgacgatcg gcgccggcgg ttcgacggtg
     3061 tgcgacgagt gccctcgcgc ccgcaatgtc aagacgatca agaagttcgt gcgcatccag
     3121 gacgtgcggg ccgacccgtc gctgtgcctg ctggagcagg gcatcccgtg caacggcctg
     3181 gccacgcgca gcggctgcgg cgcgctgtgc ccacaggcgg gcgcgcagtg catcggctgc
     3241 tatggcccgg ccgagggcgt gatcgactat ggcgcgcggt tgataacggc attcgcgtcg
     3301 gtgatcgaca gcaaagaccc ggtcgagatc gagcgcattc tcgacggcat ccccgatccg
     3361 gcggggcagt tctatcggtt caacctggcg aagtc
//
LOCUS       DBNHXB010000200         3156 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4406894, whole genome shotgun sequence.
ACCESSION   DBNHXB010000200 DBNHXB010000000
VERSION     DBNHXB010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4406894"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="AC162G_08125"
     CDS             complement(<1..1587)
                     /locus_tag="AC162G_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024882.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HIR5075817.1"
                     /translation="MAKDSTKGKSTRGGGRGKNGRKSPQGGPAIRLTLDQKLDILGLF
                     LVALALVTILSFLSSNQGDLTGAWLQFLRDTFGLGVVIVPLILGAMGLWLLARSFERI
                     PRPKASQVVGFALTFLAALTTLSWIQPATESAPGLGGEIGDWFTRLVSGAVGDFGGVI
                     VLVAWWLLGIVFLFDVTPSEMAAWIGQKIRAARAARAPGYTINTPRKANGSGQATPPK
                     VIGGAPDATTLPVRAEHAPRTAPVVEQPPPAKSIPSGKPEAPVAAPPAAPDSTPEGVE
                     PTGPLFPRIIGGGQIWRLPNIEEVFAPGSDASMSEDDIRYKVGVIEDTLHSFGVEGKV
                     VEVNRGPVITQFGMEPGFINRAGKQTKVKVSKISALADDLALALAARSIRIEAPVPGR
                     NVVGIEVPNAETSIVSLRDVIESEAFQRINSPLRFALGQDVAGQPIAADLSMMPHLLI
                     AGTTGSGKSVCVNAIVCCLLAYNTPDDLKFLMVDPKRVELTLYNGIPHLLAPVVVDLE
                     RVISVLKWVTNEMDSRYRRFS"
     gene            2021..>3156
                     /locus_tag="AC162G_08130"
     CDS             2021..>3156
                     /locus_tag="AC162G_08130"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014675.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10-like protein"
                     /protein_id="HIR5075818.1"
                     /translation="MTRRTRLTRLAALGMLAAAAIAATLLTLPTRAAHPDPSQSDPDD
                     VWARVGGLEAWYYENGYTESPPPAAPVPAAPSPMTHLSVASPTLTATAYLPLLARNHS
                     PNEFRGVWISRFDWTGFHVTPTTATIDTIVDNVKTANFNAILFQVRGTADAYYAPGLE
                     PWAARMTGELTKTLGVDPGWDPLEYLVARAHAQGIQVHAYVNVFSTWLCGLGAPPNPV
                     TPTHLFWSLSYSTTWDAWRVWTTSGPASIATCSDYLWATPALSLTRAHITAVAADLAV
                     RYDIDGIHLDLVRYPSSAYSHDPFTRQAYTDALALSPTLTFAQWQPDFQRAQVNDLVS
                     RVYSAVTTVKPDAWLSAAVWPSYTSGYNSYFQDSKGWLASGIVDA"
BASE COUNT          544 a         1076 c          979 g          557 t
ORIGIN      
        1 ggaaaagcgc cggtagcggc tgtccatctc gttcgtcacc cacttgagca cgctgatcac
       61 gcgctccagg tcgacgacga ccggcgccag gaggtgcggg ataccgttgt acagcgtcaa
      121 ttcgacgcgc ttgggatcga ccatcaggaa cttgagatcg tcgggcgtgt tgtaggccag
      181 caggcagcag acgatggcgt tgacgcacac gctcttgccc gagccggtcg tgcccgcgat
      241 cagaaggtgc ggcatcatgc tcagatcggc cgcgatgggc tggccggcca cgtcctggcc
      301 gagcgcgaaa cgcagcggcg aattgatccg ctgaaacgcc tcgctctcga tcacgtcgcg
      361 gagcgacacg atcgaggtct cggcgttggg cacttcgatg ccgacgacgt tgcggcccgg
      421 caccggcgct tcgatgcgaa tgctcctggc ggccagcgcc agcgccaggt cgtcggccag
      481 cgcgctgatc ttgctgactt tgactttcgt ctgtttccca gcgcgattga tgaagcccgg
      541 ctccatgccg aactgcgtga tcacgggtcc gcgattgacc tcgacgacct taccctcgac
      601 gccgaacgaa tgcagcgtat cctcgatgac gccaaccttg tagcggatgt cgtcctcgct
      661 catcgacgca tcgctgccgg gtgcgaagac ttcctcgata ttcggcagtc tccagatttg
      721 gccgccgccg atgatgcgcg gaaagagtgg gccggtcggc tccacgcctt ccggcgtcga
      781 gtccggcgcc gcagggggcg cggcgacagg cgcctccggc ttgccggacg gaatcgattt
      841 agcaggcggg ggctgctcca cgacgggcgc cgtgcgcggc gcatgctctg ctcgaacagg
      901 gagggtcgtc gcatcgggcg cgccgccgat cactttcggc ggcgtcgctt gaccggaacc
      961 gttcgccttg cgcggcgtgt tgatcgtgta gcccggcgcc cgcgcagcac gtgccgctcg
     1021 aatcttctgc ccgatccagg ccgccatctc gctcggcgtc acgtcgaaca ggaagacgat
     1081 gccgagcagc caccaggcga cgagcacgat cacgccgccg aaatcgccga cggcgccgga
     1141 gaccaggcgc gtgaaccaat cgccgatctc cccgcccagc ccgggcgccg attccgtcgc
     1201 cggctggatc cagctcagcg tcgtcagcgc ggccaggaac gtgagcgcga acccgactac
     1261 ctgcgacgcc tttggccggg ggatgcgctc gaacgatcgc gccaacagcc acagccccat
     1321 cgcgcccagt attaaaggga cgatgaccac gccgaggccg aacgtgtcgc ggaggaattg
     1381 cagccacgcg ccggtcaagt cgccctggtt ggacgacagg aagctcagaa tcgtcaccag
     1441 cgccagggcg acgaggaaca accccaggat atcgagcttc tggtcgagcg tcagtcgaat
     1501 cgccggcccg ccctggggcg atttcctgcc attcttgccc ctgccgccgc ccctggttga
     1561 cttacccttc gtagaatcct ttgccatagt gaaggtagta cagatgttct taagcgcaag
     1621 acattataac acatatggct tagattggta accccctcca tcgctgagac actgagaata
     1681 ctcgatggca ggtatcttgc tcggccactt gtggcgtgtc attccgaacg gagtgaggaa
     1741 tctctcctcc gaccggcgaa cacggcgcac cggggtagag atgcctcgtc gttcctcctc
     1801 ggcatgacac atctggcgca gaaggtctcc tccgctcccg ccgcgtgtgt cacccggcgg
     1861 tggcaaggat cgctgaggca ctgagacact gagaatacgc ggtgcgcttc ggctctgctc
     1921 ccgccgggtg tgtcacccgg cggccacaga ccccggacga gcgggccgga ggggatcgca
     1981 tcccctgatg cgagtcgcgc tatgatatac tctcgccact atgacacgtc gaaccagact
     2041 cacacgcctc gctgccctcg ggatgctggc tgctgctgcg atcgcggcga ccctgctgac
     2101 cctgccaacg cgcgctgctc accccgaccc gtcgcagtcc gaccccgacg atgtgtgggc
     2161 gcgcgtgggc gggctggaag cctggtatta cgagaatggc tacaccgaat cgccgccgcc
     2221 cgccgcgcca gtgccagcag ccccatctcc aatgacccat ctctctgtcg cctcgccaac
     2281 tctcacggcc acagcctact tacctctgct cgccaggaac cactcgccga acgagtttcg
     2341 cggcgtgtgg atcagccgct tcgactggac cggcttccac gtcacgccga cgacggcgac
     2401 gatcgacaca atcgtggaca acgtgaagac ggccaatttc aacgccatct tgttccaggt
     2461 gcgcggcact gccgatgcgt actacgcgcc tggtctcgag ccgtgggcgg cgcgcatgac
     2521 cggcgagctg acgaagacgc tcggcgtcga tccgggctgg gatccgttgg agtacctcgt
     2581 cgcgcgggcg catgcgcagg gcattcaggt gcatgcctac gtcaacgtct tctctacctg
     2641 gttgtgtgga ctcggcgcgc cgccgaatcc cgtcacgccg acgcatctct tctggtcgct
     2701 gtcgtattcg acgacgtggg atgcgtggcg cgtgtggaca acatcaggac cggccagcat
     2761 cgccacgtgc agcgactacc tgtgggccac gccggcgcta tcgctcacgc gcgcgcacat
     2821 caccgccgtg gccgccgatc tcgcggtgcg ctatgacatc gacgggattc acctcgatct
     2881 ggtgcgctat ccgtcgtcgg cgtactcgca cgatccgttc acgcggcaag cttacaccga
     2941 cgcgctggcg ctctcgccga cgctgacctt cgcgcagtgg cagcccgatt ttcagcgcgc
     3001 gcaagtcaac gatctggtct cgcgcgtgta ttccgcggtt acgaccgtca agcccgacgc
     3061 ctggttgagc gccgccgtat ggccgagcta cacgagcggc tacaactcat acttccagga
     3121 ctccaaaggc tggctggcga gcggcatcgt cgacgc
//
LOCUS       DBNHXB010000201         5006 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_716699, whole genome shotgun sequence.
ACCESSION   DBNHXB010000201 DBNHXB010000000
VERSION     DBNHXB010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_716699"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /locus_tag="AC162G_08135"
     CDS             <1..1526
                     /locus_tag="AC162G_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR5075819.1"
                     /translation="NPVNGAKIPIWIADYVLMTYGTGAIMGVPAHDARDFAFATKHNL
                     PIPVVIAPPNWDGRPLKEAYDGHGAMVNSGPFDGTPVPEGIQKVIRWLEEKDVGKGAI
                     NYKLRDWLISRQRYWGAPIPIIYCPEHGQVPVPEDQLPVRLPDDVEWKPTGESPLKLH
                     PTWAQTTCPVCSRPATRDTDTMDTFMCSSWYQYRYLSPQYEKGAWDPKEYDYWMPVDC
                     YTGGIEHATMHLIYTRFFTKALRDMKLVRDDEPMRQLRNQGVILGEDSEKMSKSRGNV
                     VAPDELVERYGADAVRAYLMFFAEWNKGGPWSSTGIEGVARWLNRVWNIALPAADERR
                     STKDATRNTQHEAEKALRRITHQTIKRVTGDFEDFEFNTIVSGLMEMTNALYKHRGSL
                     EGTPTWDEAVKTLLLLTAPVTPHLAEELWARRGLPYSIHQQPWPEYDAAAAAEDTITL
                     VVQVNGKVRDRIEVSPDIGEEEAKARALATDGAKRYMDGKTPKKVLYVPRRLVNIVM"
     gene            1726..2643
                     /locus_tag="AC162G_08140"
     CDS             1726..2643
                     /locus_tag="AC162G_08140"
                     /inference="COORDINATES: protein motif:HMM:NF046770.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF transmembrane domain-containing protein"
                     /protein_id="HIR5075820.1"
                     /translation="MSDRRPSLFWPILLIGIGLILLLNNAGMLQGSPWAVLWQFWPVL
                     LIVIGVDILFGRSPAGRILSAILALLVVAGAIALMAVSPAAGPGFLGFRVGGDLKTTH
                     VEYPVSDIQSADVKVSFSTGRNELHALSDSPNLIEADLRHYGELDVSYDESGSRANLE
                     IGAKGAFSVGIFQSSAEEWNVGLNPRVVYDLDLSMGVGESRIDLSQLSLSGGQIDVGV
                     GQTRMTLPDSGRFTLRVDGGVGSLRITAPREVALRAEIDTGIGSFNNNSRMRPVGDNA
                     YETEGFATADNAITLIVNVGIGSITIEDE"
     gene            2737..>5006
                     /gene="leuS"
                     /locus_tag="AC162G_08145"
     CDS             2737..>5006
                     /gene="leuS"
                     /locus_tag="AC162G_08145"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR5075821.1"
                     /translation="MPEYNPHEIEAKWLKRWSEADLYRVDLNNANRPFYNLMEFPYPS
                     GEGLHVGHFYTYSGADTYGRYQRMIGYDVFQPMGFDAFGIHGENYALQIGENPAIVMP
                     RNVARFREEQIKRMGAGFDWSREVNTTDPRYYKWTQWIFVQLFKAGLAYRAQALVNWC
                     PKDLTVLADEQVIDGRCERCSTIVVKRELEQWFFRITAYAERLLDHSKAEFTEITKTL
                     QRNWIGRSEGAEVAFQLDPSVPSVPLRGDQGTQGTQGTHQIEVFTTRPDTLWGATFMV
                     LSPEHPLVPRLTTLDRQAEVTAYGAAAAQKSEIEREDVTREKTGVFTGAYAINPVNGE
                     RIPIWIADYVLMSYGTGAIMAVPAHDQRDFEFARKYGLPIRVVIQPPDRRLDPDTLSE
                     AWPHEGVMVDSGPLDGTPADQSVQKTIEWLEAKGIGHRAVRYRLRDWLISRQRYWGPP
                     IPMIYCEKDGWQPVPDDQLPVQLPITNNFRPTGTGQSPLASIPDFVNTTCPVCGEPAR
                     RETDVSDNFLDSAWYFLRYTSTEFDDRAWDRDRAKKWLPVTHYMGGIEHTTLHHLYAR
                     FIWKAMVDLGHLPAEAGDEPFRQLRLHGWITRDGAKMSKSRGNVVNPDEYVQTYGADA
                     VRGHALFMGSYTEGGDWRDSAIAGIARFHRRVWEWVTAQSSVDSRQLSVSSEQPAVDE
                     AKARRALHKAIKKMSEDVPALGFNTAIAALMEALNVLRECRLPASTHNEIARAYVLLL
                     APIAPFLAEELWEWLGG"
BASE COUNT          984 a         1563 c         1610 g          849 t
ORIGIN      
        1 ccaacccggt caacggcgcg aagatcccga tctggatcgc cgactacgtg ctgatgacgt
       61 acggcaccgg cgcgatcatg ggagtgccgg cgcatgacgc gcgcgacttc gcctttgcga
      121 cgaagcacaa cctgccgatc ccggtcgtca tcgcgccgcc gaattgggac ggccggccgc
      181 tgaaagaagc ctacgatggc cacggcgcga tggtcaactc cggccccttc gacggcacgc
      241 ccgtgcccga gggcatccag aaagtcatcc gctggctgga ggagaaagac gtcggcaagg
      301 gcgcaatcaa ctacaagctg cgcgactggc tgatcagccg gcagcgctac tggggcgcgc
      361 cgatcccgat catttactgc cccgagcatg gacaagttcc cgtgcccgag gatcaactgc
      421 cggtgcggct gcctgacgac gtcgaatgga agccgaccgg cgaaagcccg ctgaagctgc
      481 atccgacctg ggcgcagacg acctgcccgg tgtgcagccg gcccgctacg cgcgacaccg
      541 acacgatgga cacgttcatg tgctcgtcgt ggtaccagta tcgctacctc agcccgcagt
      601 acgagaaagg cgcgtgggac ccgaaggagt atgactactg gatgccggtc gattgctaca
      661 cgggaggcat cgagcacgcc acgatgcacc tgatctacac gcgcttcttc accaaggcgc
      721 tgcgcgacat gaagctcgtc cgcgacgacg agccgatgcg gcaactgcgc aaccagggcg
      781 tgatcctcgg cgaggactcg gaaaagatgt cgaagagccg gggcaacgtc gtcgcaccgg
      841 acgagctggt cgagcgctac ggagccgacg cggtgcgcgc ctacttgatg ttcttcgccg
      901 agtggaacaa gggcgggccg tggagcagca ccggcatcga aggcgtcgcg cgctggttaa
      961 atcgcgtctg gaacatcgcg ctgccggccg cggacgaaag acggagcacg aaggacgcaa
     1021 cacgcaacac gcagcacgaa gccgagaagg cgctgcgccg gatcacccat cagactatca
     1081 agcgcgtcac cggcgacttc gaggacttcg agttcaacac cattgtctcc ggcttgatgg
     1141 agatgaccaa cgcactgtat aagcatcgcg ggtcgctgga aggcacgcca acctgggatg
     1201 aggcggtcaa gacgctgctg ctcctgacgg cgccggtcac gccgcacctg gccgaagaac
     1261 tgtgggcgcg gcgcgggctg ccgtactcga tccatcagca gccgtggccg gagtacgatg
     1321 ccgctgcggc agccgaagac acgatcacgc tggtcgtgca ggtcaacggc aaggtgcgcg
     1381 atcgtatcga agtgtccccg gacatcggcg aggaggaagc gaaggcgcgg gcgctggcga
     1441 ctgacggcgc gaaacggtat atggacggca agacgccgaa aaaagtgctc tacgtgcctc
     1501 gaaggttagt gaatattgtc atgtaaggca caggcgctcc cgccgcgtgg cctggcggtg
     1561 ggagcgcctg gtttctcggg cgggacacaa gtgtttggca gcccgatccc tacgccgttc
     1621 gtcccgggcc gacatccgcc tctagtccgt tgacgcccgg cgcaatcggt tcgatcatag
     1681 acatggatac ttcccagaac atctctcgaa gaaggagcac ataccatgtc tgatcgtcgt
     1741 ccaagcctct tctggccgat cctcctgatc ggcatcgggc ttatcctgct gctgaacaac
     1801 gccgggatgc ttcagggcag cccgtgggcc gtcctctggc aattctggcc ggtgctgtta
     1861 atcgtgatcg gggtggatat tctgttcggc cggtcgccgg ccggacgcat cctcagcgcg
     1921 atcctggcgc tgctggtcgt cgccggcgcg atcgccctga tggccgtctc gcctgcggcc
     1981 gggcctggct tcctcggctt tagagtcggc ggcgacctga agaccacgca cgtcgagtat
     2041 ccggtgagcg atatccagtc cgccgacgtc aaggtcagtt tctcgaccgg caggaacgag
     2101 cttcacgcgc tgagcgattc gcccaacctg atcgaagccg atctgcgtca ctacggcgag
     2161 ctggacgtct catacgacga gtcgggaagc cgcgccaatc tggaaatcgg cgccaaaggc
     2221 gcgttctcgg tgggcatctt ccagtcgtcg gcagaagagt ggaatgtcgg cctgaacccc
     2281 agggtcgtct acgatctcga cctgagcatg ggtgtgggcg aatcgaggat cgatctgagc
     2341 cagctttcat tgagcggcgg tcagatcgac gtcggcgtcg gtcagacccg gatgacgctg
     2401 cccgactcgg gccgattcac gctgcgcgtc gatggcggcg taggctcgct gcgcatcact
     2461 gcgccgcgcg aggtcgccct gcgcgccgag atcgacactg gcatcggctc gttcaacaac
     2521 aactcgcgca tgcgcccggt cggcgacaac gcatacgaga ccgagggctt cgcaacggcc
     2581 gacaatgcga tcacactgat cgtcaacgtg ggcatcggat cgattacgat cgaagacgaa
     2641 tgacatcgta agtcgtgata cgtgatgcgt gatgcgcgat cgtgatcgcg catcacgtac
     2701 tgtgttttgg atggtgggcc aggggcagga ggcgcgatgc cggagtacaa cccgcatgag
     2761 atcgaggcga aatggctgaa gcgctggagc gaagctgact tgtatcgggt cgatctgaac
     2821 aacgccaatc ggccctttta caatttgatg gagttcccct acccgtccgg cgaagggctt
     2881 cacgtcggcc atttctacac ctatagcggc gcggatacgt atggccgcta ccagcgcatg
     2941 atcggctacg acgtgttcca gccgatgggc ttcgacgcgt tcggcatcca cggcgagaac
     3001 tacgcgctgc agatcggcga gaacccggcc atcgtcatgc cgcgcaacgt cgctcgcttc
     3061 cgcgaagagc agatcaagcg catgggcgcg ggcttcgact ggtcgcgcga agtcaacacc
     3121 accgatccgc gctactacaa gtggacgcag tggatcttcg tccagttgtt caaggccggg
     3181 ctggcgtatc gggcgcaggc gctcgtcaac tggtgcccca aggacctgac cgtgctcgcc
     3241 gacgagcagg tgatcgacgg gcgctgcgag cgatgcagca ccatcgtcgt caagcgcgaa
     3301 ttggagcagt ggttctttcg gatcacggct tacgccgagc ggctgttgga tcattctaaa
     3361 gccgagttca ccgagatcac caaaacgctg cagcgcaact ggattggccg cagcgaaggg
     3421 gccgaggtgg ctttccagtt agacccctca gttccttcag ttcccttacg aggagatcaa
     3481 ggaactcaag gaactcaagg aactcatcaa attgaggtgt tcactactcg cccggacacg
     3541 ctgtggggcg cgacgtttat ggtgctgtcc ccagaacacc cgctggtccc cagactgacc
     3601 acgctcgacc gtcaggcgga agttacggcg tacggcgctg ccgccgcgca aaagagcgag
     3661 atcgagcgtg aagacgtgac acgcgaaaag accggcgtgt tcaccggcgc gtatgccatc
     3721 aacccggtga acggcgagcg catcccgatc tggatcgcgg actacgtgct gatgtcgtat
     3781 ggcaccggcg cgatcatggc cgtgcctgca cacgatcagc gcgacttcga gttcgcgcgc
     3841 aagtacggcc tgccgatccg cgtcgtcatc cagccgcccg accgccggct cgatcccgac
     3901 accctgagcg aggcctggcc gcacgaaggc gtcatggtcg attccggccc gctcgacggc
     3961 acacccgccg accagtcggt gcagaagacg atcgaatggc tggaggcgaa gggcatcggc
     4021 catcgtgccg tgcgctatcg cctgcgcgac tggctgatct cgcgccagcg ctactggggt
     4081 ccgccgatcc cgatgattta ctgcgagaag gacggctggc agccggttcc cgacgatcaa
     4141 ctgcccgtcc aattaccaat aaccaataac ttccgcccca ccggaacggg ccagtcacca
     4201 ctcgcttcga tcccggattt cgtcaatacg acctgtcccg tctgcgggga gccggctcgc
     4261 cgcgagaccg acgtgagcga caacttcctc gactcggcct ggtatttcct gcgctacacc
     4321 tcgaccgagt tcgacgatcg ggcctgggac agggatcggg ccaagaagtg gctgccggtc
     4381 acgcactaca tgggcggcat cgagcatacc acgcttcatc acttgtacgc gcgcttcatc
     4441 tggaaggcga tggtcgatct cggccacctc cccgccgaag ccggcgacga gccgttcagg
     4501 caactacggc tgcacggctg gatcacccgc gacggcgcga agatgagcaa gagtcgtggc
     4561 aacgtcgtca accccgacga gtacgtccag acgtacggcg ccgacgcggt gcggggacac
     4621 gcgttgttca tgggctccta tacggaaggc ggagactggc gcgattcggc gatcgccggc
     4681 attgcgcgtt tccaccggcg cgtgtgggag tgggtcactg cacagtcatc agtggacagt
     4741 aggcagttat cagtgagcag cgagcagccg gcagtggacg aggcgaaggc tcgccgcgcg
     4801 ctgcacaagg cgattaagaa gatgagcgaa gatgttcccg cgcttggctt caacacggcg
     4861 atcgccgcgc tgatggaagc gctcaacgtg ctgcgcgagt gccggctgcc ggcgtcgacg
     4921 cacaacgaga ttgcgcgggc ttacgtgctg ctccttgcgc cgatcgcgcc gttcctggcc
     4981 gaagaactgt gggagtggct gggcgg
//
LOCUS       DBNHXB010000202         5420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3736436, whole genome shotgun sequence.
ACCESSION   DBNHXB010000202 DBNHXB010000000
VERSION     DBNHXB010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3736436"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..611
                     /gene="hepT"
                     /locus_tag="AC162G_08150"
     CDS             153..611
                     /gene="hepT"
                     /locus_tag="AC162G_08150"
                     /inference="COORDINATES: protein motif:HMM:NF047751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII toxin-antitoxin system HepT family
                     RNase toxin"
                     /protein_id="HIR5075822.1"
                     /translation="MDARTADVIRKKLDYLEKTLVEIEPYLKAGYAEYTRQPAYRRAT
                     ERLVQIIIEAAGDTSELLLQAAGRSAPGTMREAFGGIRDLGAIDDALFERFNRTYVGL
                     RNRIVHDYETLDNRILFDSARRLRKDARAFLKSVTAYLTSMSSKKSDHKR"
     gene            648..2054
                     /gene="lpdA"
                     /locus_tag="AC162G_08155"
     CDS             648..2054
                     /gene="lpdA"
                     /locus_tag="AC162G_08155"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR5075823.1"
                     /translation="MATTYDVTIIGGGPGGYVAAIRAAQLGLKVALVEREYLGGVCLS
                     WGCIPSKALLRNAEIAQLLGRGKEFGFSFDNLKLDYAVAQKRSRQVSERLVKGTQFLM
                     KKNKVDVYQGSGKLKSASQVEVISAPDAPGVNGQKFDGVLDTKNVVIATGARAAHIPG
                     VQVDGQSIITYRHALELTEAPKSMVIIGAGPIGLEFGYVFRSYGAEVTIIEMLPAVAP
                     KEDEEVSAELAKQFTRMGFKLMANTKVDSVTKIDGGVEVKAGGQVIKADKALVATGFT
                     PNTENIGLEAVGVRVERRAIVVDGKYRTSVPNVYAVGDVVGGLMLAHVGSAQGIVAAE
                     TIAGHEAPELDENALPRCTYCIPQIASMGLTEKQCKEQGIEVNVGKFPFQPNGKALGL
                     GEGRGFVKVIADKKYGEILGVHMIGPEVTELLPEWVLARNYELTPSEIARSVHAHPTL
                     TEVMMEAAHAVEGQAIHI"
     gene            2260..3423
                     /locus_tag="AC162G_08160"
     CDS             2260..3423
                     /locus_tag="AC162G_08160"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR5075824.1"
                     /translation="MSRPLLMIPGPVEVDETVLQALGQPPLSHTAASFIEIFGACLDA
                     LREVFRAPDGQPFVVAGSGTLAMEMAVANLIEPGDRAVVVDTGYFSHRMADVLARHGA
                     VLESVSAPAGDCPSLERVESALKAGAKVLAITHVDTSTGVLAPVPDLAALARRYNALT
                     VVDGVCALGGETFEMQAWKVDVCLTASQKALAVPPGLALVMAGPRAIEAFEARRRPAG
                     SYYCDWDLWLPIMHAYQARKPSYFGTPPTNLIVALYESVKQILKEGMEARWARHRKLG
                     ASMRAAMQALGLKWVPARPDLCADTLTTIYYPDGVDDTLVGRIGQHGVAVAGGLHPDI
                     KGRYFRIGHMGILCDAEIRQTVAAVEAALKDAGYLGENGAGVTASQAAYEHAR"
     gene            3443..5137
                     /locus_tag="AC162G_08165"
     CDS             3443..5137
                     /locus_tag="AC162G_08165"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5075825.1"
                     /translation="MSLRDQANERKLRAQLEALNEASIAITSELGLERVLQRIVDMAR
                     DLAGARYGALGVPGAHGELEQFVVSGMTPEEVKRVGHPPRGKGLLGVLLREPNPIRVA
                     DIAADPRSAGFCENHPHMTSFLGVPIVSHGRLLGNLYLTDKIDAPEFSEDDERLIGML
                     ATHAAIAIDNARLVREALERGRQLAQRNQELEALNAVVTTASQSLDLESLLHDTLDKV
                     LSVLQAEAGEIFLREETSGDMVLVVHRGSFPSAFYSLNRFESGQGFIGIVATTGEPLI
                     TGELHRDTRFVREEVVRAGIRSFACIPITVKNQVIGVMSLASMQPDGFAEHNLDLLAA
                     IGRQIGVAVENARLYSEVKRLAVVDERARIGMELHDGIIQSIYAVGLTLEYASLMLED
                     HPQVTRERLGQAIDGLNEVIADLRSYILDLRPQRFEGKDLATGLLELVRTFRANTFVG
                     VELAINQAASAQVKPDLAAGLFHIAQEGLANVAKHARARNVKLSLDHVSGVLILEIKD
                     DGRGFDPQAVTRYAGHGMRNMEERARLFNGELRVDSAPGRGTRIQVRVPLAPASQA"
BASE COUNT         1010 a         1698 c         1797 g          915 t
ORIGIN      
        1 ttgctgttcg aggtcgccag cgacggcgtg ccgctgttcg aggcggagcc ggccggcttc
       61 gcccagttcc gatcctacgc cgcacgcgtc tacgacgata atcgcaagtg gcgcgaacgt
      121 cgccgccgat accaagagcg taaatacgcc tgatggatgc ccgcactgcc gacgtcattc
      181 gcaagaagct ggactacctc gaaaagactc tcgtcgagat cgaaccctac ttgaaggcgg
      241 gttatgccga gtatacccgg cagccagcct atcggcgcgc gaccgagcgg ctggtgcaga
      301 tcatcatcga agccgccgga gatacaagcg agttgctgct gcaggcagcg ggccgatcgg
      361 cgccgggcac gatgcgcgaa gccttcggcg gcatccggga cctcggcgcc atcgatgatg
      421 cactgtttga acgctttaat cgcacttacg tcggcctgcg caaccgcatc gtccacgact
      481 acgagacttt ggataaccga attctgttcg actcagccag gcgcttgcgc aaagacgcgc
      541 gggcatttct caaatcagtc acggcctatt tgacttcgat gagctccaag aaatcagacc
      601 ataagcgata agcgatacgc tatcggcgat aagcgacagg aggcagagtg gcaaccacct
      661 acgatgtgac gatcatcggc ggcgggccag gcggctacgt cgccgcgatc cgcgccgcgc
      721 agttggggct gaaggtcgcc ctcgtcgagc gcgagtatct cggcggcgtg tgcttgagct
      781 ggggttgcat accctcaaag gcgctgctac gcaatgccga gatcgcccaa ctcctgggac
      841 gcggcaagga gttcggcttc tcgtttgaca acctgaagct cgactacgcg gttgctcaga
      901 agcgcagccg gcaggtcagc gaacgcctgg tgaagggcac gcagttcctg atgaagaaga
      961 acaaggtcga cgtgtaccag ggcagcggca agctcaaatc ggccagccag gtggaagtga
     1021 tctccgcgcc cgatgcgccc ggcgtcaacg gccagaaatt cgatggcgtg ctcgatacga
     1081 agaacgtcgt catcgcgacc ggcgcgcgcg ccgcgcacat tccgggcgtg caggtcgacg
     1141 gccagtcgat catcacctat cgccacgcgc tcgagctgac tgaagccccc aagtccatgg
     1201 tgattatcgg cgcggggccg atcgggctgg agttcggtta cgtcttccga tcttacggcg
     1261 cggaagtgac gatcatcgag atgctgcccg ccgtcgcgcc gaaggaagac gaggaggtca
     1321 gcgccgagct ggctaagcag ttcacgagga tgggcttcaa gctcatggct aacacgaagg
     1381 tcgattcggt cacgaagatc gacggcggcg tcgaagtgaa ggccggtggg caagtgatta
     1441 aagcggacaa agcacttgtc gcgaccggct tcacgccaaa cacggagaac atcgggctgg
     1501 aggcagtcgg cgtcagggtc gagcgccgcg cgattgtcgt cgacgggaag tatcgcacca
     1561 gcgtcccgaa tgtgtacgcc gtcggcgacg tggtcggcgg gctgatgctg gcgcatgtcg
     1621 gatcggcgca aggcatcgtc gccgccgaga ccatcgccgg ccacgaagcg ccggagctgg
     1681 acgagaacgc gttgccgcgc tgcacgtact gcatcccgca gatcgcgtcg atggggttga
     1741 ccgagaagca gtgcaaggag cagggcatcg aggtcaacgt cggcaagttc ccgttccagc
     1801 ccaacggcaa ggcgttgggg ctgggcgagg gccgcggctt cgtcaaggtc atcgccgata
     1861 agaaatatgg cgagattctg ggcgtgcata tgatcggccc cgaagtgacc gagctactgc
     1921 cggagtgggt gctggcgcgc aattacgagc tgacgccgag cgagattgcg cgctcggtgc
     1981 acgcgcaccc gacgctcacc gaggttatga tggaagccgc gcacgcggtt gaaggacagg
     2041 cgattcatat ttagaggctg gaggttagag attagaggtt ggaggttgga gccgcatcca
     2101 ggcgcggctc ttttggttct tgacagggtg gacaagtagg gccacaatcc tatcgcgtcg
     2161 gcgattggcg gatgatctac cgtttggaca agggatttgt ctacttcaag gtcattcgct
     2221 accggcgaga cgtctatctt cacaaataag aggccaatca tgtctagacc cttgctgatg
     2281 atccccggac ctgtcgaggt cgacgagacc gttttgcagg cgctcgggca gccgccgctc
     2341 agccataccg cggcgtcctt cattgagatc ttcggtgcgt gcctcgatgc gctgcgcgaa
     2401 gtgttccgcg cgcccgacgg ccagccgttc gtcgtcgccg gttccggcac gctggcgatg
     2461 gagatggccg ttgcgaacct gatcgagccg ggcgaccgcg ccgtcgtcgt cgacaccggc
     2521 tatttctcgc accgcatggc cgacgtcctc gcccgtcatg gcgcggtgct cgagtccgtc
     2581 tccgcgccgg ccggcgattg cccgtcgctc gaacgagtcg aatcggctct gaaggcgggc
     2641 gcgaaggtct tggcgatcac gcacgtcgac acgtcgaccg gtgtcctcgc gccggttccc
     2701 gacctggccg cgctggcccg ccgctacaac gccctgaccg tcgtcgacgg cgtgtgcgcg
     2761 ctgggcggcg agacattcga gatgcaggcg tggaaagtcg acgtctgcct gaccgcgtcg
     2821 cagaaggcgt tggccgttcc gccgggactc gcgctggtca tggccgggcc gcgcgcaatc
     2881 gaggcattcg aagcgcgcag acgccctgcc ggctcatact attgcgactg ggacctgtgg
     2941 ctgccgatca tgcacgccta ccaggcgcgc aagccctcat acttcggcac gccgccgacg
     3001 aacctgatcg tcgcgctgta tgagtcggtc aagcagatcc tgaaggaagg catggaagcg
     3061 cgctgggcgc ggcatcggaa actcggcgcg tcgatgcggg cggcgatgca ggctcttgga
     3121 ctgaagtggg tgcccgcccg gccggacctg tgcgccgaca cgctcaccac catctattat
     3181 cccgatggcg tggacgacac gctggttggc cgcatcggcc agcacggcgt cgcggttgcg
     3241 ggcggcctgc atcccgacat caaaggaaga tacttccgca tcgggcacat gggaattctg
     3301 tgcgacgccg agattcggca gacggttgcg gcagtcgaag cggcgctgaa ggatgccggt
     3361 tatcttggcg agaacggcgc gggggtcacg gcgtcgcagg ctgcctacga acacgcccgc
     3421 tgatctcggc tataatcgcc gtatgtcttt acgcgatcag gccaatgaac gcaagctgcg
     3481 cgcgcagctc gaagcgctca acgaggcttc gatagccatt acgtccgagc tgggcctgga
     3541 gcgcgtgctg cagcgcatcg tcgacatggc tcgcgacctg gccggtgcgc gctacggcgc
     3601 gctcggcgtg cccggcgcac acggcgagct ggagcagttc gtcgtttccg gcatgacgcc
     3661 ggaagaggtc aagcgcgtcg gtcacccgcc gcgcggcaag ggactgctcg gcgtgctgct
     3721 gcgcgagccg aacccgattc gcgtcgccga catcgccgcc gatccgcgct cggccggctt
     3781 ctgcgagaac caccctcaca tgacatcgtt cctgggtgtg ccgatcgtat cgcacggcag
     3841 gctgctgggc aacctgtacc tcaccgacaa gatcgacgcg cccgagttca gcgaagacga
     3901 cgagcggctc atcgggatgc tggcgacgca cgccgccatc gccatcgaca acgcgcggct
     3961 ggtgcgcgag gcgctcgagc gcggccggca actggcgcag cgcaaccagg agcttgaagc
     4021 actgaacgcc gtcgtcacga ctgcgtcgca gtcgctggac ctggaatcgc tgctgcacga
     4081 cacgctcgac aaggtgctgt ccgtgctgca ggccgaggcc ggcgagatat ttctgcggga
     4141 ggagacttca ggcgacatgg tgttggtcgt ccaccgaggc tccttcccca gcgccttcta
     4201 ctccctgaat cgattcgaga gtgggcaggg cttcatcggc atcgtggcga ccaccggcga
     4261 gccgctcatc accggcgagc tgcaccgcga cacgcgcttc gtgcgggaag aggtcgtgcg
     4321 cgccggcatt cgatccttcg cctgcatccc gatcaccgtc aagaaccagg tcatcggcgt
     4381 gatgtcgctg gcgagcatgc agccggacgg cttcgccgag cataacctgg atctcctcgc
     4441 ggctatcggg cggcagatcg gcgtcgccgt cgagaacgcc cggctctaca gcgaagtcaa
     4501 acggctggcg gtagtcgacg agcgggcgcg catcgggatg gagctgcacg acggcatcat
     4561 ccagtcgatc tacgcggtcg ggctgaccct cgaatatgct tcgctgatgc tcgaagatca
     4621 cccgcaagtg acgcgcgagc gcctgggcca ggccatcgac ggactgaacg aagtgattgc
     4681 cgacctgcgc tcctacatcc tcgatctccg gccgcagcgc ttcgaaggca aagacctggc
     4741 caccggcctg ctcgagctgg tgcggacgtt tcgggccaac accttcgtcg gcgtcgagct
     4801 ggcgatcaac caggccgcca gcgcgcaagt caagccggac ctggccgccg ggctttttca
     4861 catcgcacag gagggattgg ccaacgtcgc caagcacgcg cgggcgcgca acgtcaagtt
     4921 gtcgctcgac cacgtcagcg gcgtgctgat cctcgagatc aaagacgacg gacgcggctt
     4981 cgatccgcag gccgtgacgc gctacgcggg ccacggtatg cgcaacatgg aggagcgagc
     5041 gcgcctgttc aacggggagc tgcgggtcga cagcgcgccg gggcggggca cgcgcataca
     5101 ggtgcgcgtg ccactcgcgc ccgcctcaca ggcctgagcc gctcgcgtta catccgtcag
     5161 gcgcaagtgg gacgaatgac actccaaacc cgagggtgtg acagcctata cttttcagca
     5221 tcaatacagg ttgagccatt ttcttcccgg gcagagggac attcgcgccg tggtgacact
     5281 ggttatcatc gatcatcacg catcgatccg gcaggcgctc gcgcagcgcc tcagccggat
     5341 tccggacatt caggtggtgg ggacggcggc ggatggcctg gaggggatcg cgcaggcacg
     5401 cacacaccgc cccgacgtcg
//
LOCUS       DBNHXB010000203        20076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2226971, whole genome shotgun sequence.
ACCESSION   DBNHXB010000203 DBNHXB010000000
VERSION     DBNHXB010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 20076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20076
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2226971"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="AC162G_08170"
     CDS             <1..1254
                     /locus_tag="AC162G_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR5075826.1"
                     /translation="DAIRVGLIYKPGKVAPVGDFKLLTTAVDPRFLDTKNRPALAQTF
                     EELATGARFTVVVNHLKSKGSDCNDVGDPDIGDGQGNCNQTRKAAAQALVDWLATDPT
                     GSGDSDFLIMGDLNSYAKEDPIDAIQAGADDTAGTGDDYTNLIAQYQGTYAYSYVFDG
                     QSGYLDHALASPSLAAQVTGAADWHINADEPDLLDYDTTFKPAAQEAIYEPNAYRSSD
                     HDPVVVGLGLALNATCNGLPATLVGTTGDDVINGTSGPDVIAGLGGNDIIYGGKGDDV
                     ICGYAGDDTLNGDDGNDTLIGSYGNDVLDGGKGNDTLTGGDGSDVLDGQDGEDVLDGG
                     SGNDTLMGGDGDDTLAGGNGNDALTGSNGDDVLDGGADNDTLVGNKGDDSLTGGTGAD
                     SFSGGLGTDTNIDFNASEGDTSDGT"
     gene            complement(1409..2410)
                     /locus_tag="AC162G_08175"
     CDS             complement(1409..2410)
                     /locus_tag="AC162G_08175"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR5075827.1"
                     /translation="MSEGNGSHPPVLLLYNYDSTWTAEDVLDCMQHVERMAGGLRNQG
                     HAVQPVEVRADIVDPLRVYDPREAIVFNWCEGIDGAPNAYDLVARTLDDMGFTYTGSD
                     AWALQHTQNKAEFKKILERLGIATPRWRVFTDTRDTDEWTTFPAIVKPVAEHCSYGIT
                     ADSVVDDVAGLHRQIDTLMEKFGVGALAEEFIAGREINVAIWGNGAPQALPLYEIDFG
                     DIEDPRRRVVDYDSKWVPTSFQYTHTPSRCPAELEEPVAEAIRTTALAAYRALRLRDY
                     GRIDTRVRDGVPYVVDVNANCDITSDGGFAKTAKVAGYDYGAMASQIVGWAARRKPM"
     gene            complement(2492..3013)
                     /locus_tag="AC162G_08180"
     CDS             complement(2492..3013)
                     /locus_tag="AC162G_08180"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5075828.1"
                     /translation="MRAVLQTIPPSPWLATGSVQPTSPDEREQIVQVAIEAAVFTERE
                     VATVAEMLDAYYDDPEVSGYHFLSYREDGRVLGFASWGPRDLAMHAFDLYWIAARPDV
                     QRRGVGRALMEAVEAQARACGGGWMWVETSDTPAYAAARALYERCGYRRLVRLPDFYR
                     SGDGLVIYTKRVE"
     gene            complement(3010..4041)
                     /locus_tag="AC162G_08185"
     CDS             complement(3010..4041)
                     /locus_tag="AC162G_08185"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR5075829.1"
                     /translation="MAPHRPLRRVLVAYSAIERTVRGEPRELEAELETIQTAQTIAEN
                     LNRAQIRAQTYVVYGLSDVDRIASEFDVETTLVFNLCEHLQGDSHRDGEVAQRMTDLG
                     IQFTGASVGTLASSLNKARTKEMLRAAGVPTARYQVFSQPDDPIRVPLPAIVKPVAED
                     ASIGIDRDSVVHDELSLRRRVEYVLDAYRESALVEEFIDGREFNVGLWGNGRRYMLPI
                     AELDYCDWADPYQRFLHFDAKWNPQAPEYQTMYVRCPADIDNALAERVGKTARMAYRV
                     MGCRDYGRVDMRLKDGRPMVLEVNPNPCLAPDAGFPKAARAAGYDYPQMVARIARWAW
                     ARRGKEDAL"
     gene            complement(4106..5218)
                     /locus_tag="AC162G_08190"
     CDS             complement(4106..5218)
                     /locus_tag="AC162G_08190"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR5075830.1"
                     /translation="MSEPDKEFEECDSTRSGRSLRVALLVNLASNAPEPPPGAPHDYL
                     YELDHENNVAAYHAALEARGYTVFPMEGNAALPARLRGTPVDICFNTCEGFRGDAREA
                     QAPALLEMLGVPYTGSQVRALALTLDKPMTKRVLAFHGLPTPAFQEFRSPGEPLDPTL
                     RFPLFAKPAREGTGMGIDERSVLHTEAEVRDRVTYLIHAYRGPALVEEYIEGRDVTVG
                     MVGNWPDPHVFPISMVDYSGYGERAVPIYTAEYKVDRADDYRYQCPADLPGSLAGELV
                     RLAVETMRVTNTLDFARVDFRLNRLEGDKPYILEINSLPGITPISDLTLMAAADGWSH
                     ADLVNGILDAALRRLGLVEAISLKEERASNNGRKPG"
     gene            complement(5249..6637)
                     /gene="kamA"
                     /locus_tag="AC162G_08195"
                     /gene_synonym="ablA"
     CDS             complement(5249..6637)
                     /gene="kamA"
                     /locus_tag="AC162G_08195"
                     /gene_synonym="ablA"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019011019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - L-lysine 2,3-aminomutase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HIR5075831.1"
                     /translation="MTNRILEKPRKRAAGFEDVPDSTWNNWRWQMSNRLNTVEELSRI
                     VTLTEGEIKALTTKNLFRVDITPYFASLIAPDDPHDPIRKQVVPTARELDAFESMMED
                     SLAEDRHSPVPGLVHRYPDRVLFLVTTQCASYCRYCTRSRIVGEPTENFSRKEWDAAI
                     DYIARTPQVRDVLLSGGDPLALAPKLLDGLMSRLREIPHVEIIRIGSRVPVFLPQRVD
                     DEFCQMLAKYHPVWMNIHVNHPNEITPELYEACDRLSRAGVPLGNQSVLLAGVNDSIH
                     IQRQLVHELVMMRVRPYYLYQCDLVMGAGHFRTSVSKGIEIIEGLRGHTSGYAVPTYV
                     IDAPGGGGKIPVMPNYVISQAPGKVVLRNFEGFITTYTEPLDYDPHEIEWLDRQAPRR
                     LEPGQEGVAALLEGERMTIEPEGFEDVHTRGGGQHRLRADESKWQPLGIGTEHKPVLK
                     GAQAPSDNGGGA"
     gene            complement(6983..7654)
                     /locus_tag="AC162G_08200"
     CDS             complement(6983..7654)
                     /locus_tag="AC162G_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5075832.1"
                     /translation="MTELADGAKRRRQRVMILAEEIVAARWAQALAEDGVVCLIAANV
                     SAAAKVLHHESALELMLVDRAFLDRQFQALTRMFVSVGRWPVIVPIKNGGRKPTKVDR
                     SRVGAALTLLRSSRRSAEWLRLGDLTIDLARKRAKFRRRKWVQLPPMQYQILVTLAQR
                     PNEVLGFRELLRQVWGYDGSKAEAQELLKAHVRLIRRKMRWNPQTGEYLRSVRGFGYM
                     LSVPE"
     gene            7688..8545
                     /locus_tag="AC162G_08205"
     CDS             7688..8545
                     /locus_tag="AC162G_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013078.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HIR5075833.1"
                     /translation="MIDQQEQRPARQPPALASRHEQGTIPVDSLAHPSAMPTPVQHLV
                     IADAILAKSSLPEHARAFLNAQRAEFLFGNVAPDVQTVSGQPRAATHFFNVPLDSSRP
                     AHEVMFEAHTHLSEPGRLPAGQAAFLAGYIMHLLLDVMWVRDIFQPIFGPDAGWSTFH
                     DRLFLHNVLRAWCDRRDQAHLAGNAGASLATVTPNHWLPFTGDRYLRQWRDALVEQLA
                     PGATIRTVQVFAERGQIDPLAFERVLESEDELNDRIFSQAPRRVIDDFYTRGLSESCA
                     LLNDYLARV"
     gene            8917..9903
                     /gene="galE"
                     /locus_tag="AC162G_08210"
     CDS             8917..9903
                     /gene="galE"
                     /locus_tag="AC162G_08210"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR5075834.1"
                     /translation="MKILVTGGAGYVGSHCVEELCEAGEDVVVFDNLYYGHREAVHPK
                     AKFVEGDLKDADALKRLFDAQPCDAVMHFASHTLVGESMEKPLLYLGDNITNALNLFR
                     AMIDHGVRRFILSSTANLFDKPERIPIDEDERIVPGSPYGESKNIIERQLYWLDKIYG
                     IRYACLRYFNASGASPTGLRGEDHEPETHLIPITLQVALGQREKLIVFGNDYPTPDGT
                     CVRDYVHVNDLAQAHILALQALDQGSRTYNLGNGNGYSNLEVLQTAEAVTGRKIAWEF
                     GPRRRGDPAVLVASSERIQRELGWKPRYGSLRDIISTAWEWHRAHPNGYDGK"
     gene            9916..11067
                     /locus_tag="AC162G_08215"
     CDS             9916..11067
                     /locus_tag="AC162G_08215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5075835.1"
                     /translation="MHIVINGWFLNQPNTGSGQYLRQLITRLPRLAPQHEFILVVPDR
                     RSFRVFDLHTDANLQSLISNLQPPTSDFRKLLFEQSIIPRAARALRADLIHVPYWAPP
                     LRASVPIVVTIHDVIPLVLKEYRGGPLGRLYTGLVTAAARGAALTLTDSEASKRDILQ
                     HLGVSESRVRTIYLAADPKFTPRRDPVDHAAVRRKYDLPDNYVLYLSGFDPRKNVEAA
                     LQVYTWGQDAIGHSHPLVVAGRLPEGGDGFFTDPREIVKRIEIEDVVQFIGEVDEEDK
                     VALYQGASAFLYPSRREGFGLPALEALACGVPVIGSEASSIPEIVGDAGMLVSPDDAR
                     AMAGALIAVVTEPPLREALSERALKQAARFSWDKTARETVEAYESVMKA"
     gene            11078..13483
                     /locus_tag="AC162G_08220"
     CDS             11078..13483
                     /locus_tag="AC162G_08220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 117 protein"
                     /protein_id="HIR5075836.1"
                     /translation="MTTSSLISLGKRQREILRPALFDVALLFAAFLLYTLTLTPSLLP
                     ADSGEFQLVAWKLGIAHPPGYPLYIVVGHLFSRFFASPAFALNVLSAILASITLVLVS
                     RAVRAATGSTLGGLAAAVILATATTFWAQATTANIRMPTALFTAACVYVLVMHQQAVA
                     EARNTAAAAPPRLRAGSPDRYLILFALLFSLGLSHHVSLIFPGIFFVTFVLLVDPSIF
                     RQPRRWIRPGIAFLLGLLPLLYLPARGLAGGTLANGESGAELAQPDRFLDYVLARGFE
                     GDFFYFVTERPDLLDDRLALLPTLFDFQFNLGALMLGLLGAVVLARRNWRLLVMLLGG
                     IAAHTFVTLAYRAPQTVEYLMPAYVLFAILIGCSVGQVLTTETRTLGAQRFSLWFNLV
                     LFVMALIAGLARGLDNLPSYDWLSRNGDTRAYAESLLEDAPPDAIILSNWHWANPMWY
                     LQQVERLRADVDVVYVYPRGEPLAITWLKEIDSSVTTGRPVIVDMYFHEEFLASPYFF
                     EPISQEAFLVRQSYLSNLPQGYTPYEAEFGGKFKLLGFRLLDERTTPGEPYMVNLAWR
                     VESQPERDYSFFVHLVDSSGRVVGQADRTLPTMRYKPGDVIVERFFVAPLPGVLPDEY
                     TLATGIYTVENTEIVELGGQAQFARTIVEPPVRLADQPTSAVALSHGIYFLNPLPYPR
                     ASALYPGSQLVLDLRFLASRPLTRDIVVSVQMIGEGYTWKTTADSVPALGAIPTLKWI
                     AGSQVADRHVVDIPPDTPPGPATVSVILYDHFTQQPLALLDPQLIQQDRSIPLGAWTI
                     LPP"
     gene            13582..14607
                     /locus_tag="AC162G_08225"
     CDS             13582..14607
                     /locus_tag="AC162G_08225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5075837.1"
                     /translation="MADHLDLSIIIPARNEAESLPSLHAEIGAALAPLHKSYEIIVVD
                     DGSTDDTFAALRRLAECDPHVVAVRLRRSFGQTAALVAGFDLACGDVVVTMDADGQND
                     PADVPKLLDKIDEGYDIVSGWRVNRKEPFITRRIPSMIANRILTARTGVRLHDSGCSL
                     KAYRSEVVRHIRLYGEMHRFIPALASSMGVTVAEVPVNDRARKHGKSKYGLSRTFRVI
                     LDLFTLNFLLGFQGKPMQLFGCAGLITGAIGFAILAYLTFLKIAYGEQLSNRPVLWLG
                     VLLGIVGVQFLFFGLIAEMLTRTYHESQGKSIYVIREIVGRDAIHEPPHERVGEPANS
                     PHDSLTT"
     gene            14626..17154
                     /locus_tag="AC162G_08230"
     CDS             14626..17154
                     /locus_tag="AC162G_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075838.1"
                     /translation="MRPSIRRRLRRDLFVLLFFTLLTLVLTWPLVARIGDAYAGNNED
                     LWVFQWDNWWTRVALQQGLDPLYTPYQFYPGGVSLAAHSLSFYNSLIWIPLAALFGDI
                     AAYNITILLTFILSGYMMFKLAEYLLPDARPPLPALLAGVVFAFAPYHFSQSLGHVSL
                     ASVQWFPLLALFILKTARERAWRNVVAIGLLALLIAATRLQFLILGGVVFALFVGVDW
                     LVSRHEWSRGALARLIAGAAAGLTLCLPLLVPAARLYTQAASPDELIADEDEWGQTDL
                     LAYAVPMTYHPIFGAAVQPLYQRFVKNQAWMPFLGYTAVALAAWGIARLRRRAWPWVA
                     IGAFLFVMALGPTLRVNGMVYEDIRLPYALIGDWFPLNTLRSPDRYNLLMPMALAALA
                     GAGADGVLRRWASRRGTIWVGLLGTLIVFEYLGLPYPTIEPLPTSPYFDALAADPEPY
                     AILDIPLERSDTKRYLYYQTRHGKPIVEGRVARVPAEAYALFDAIPLLDGWRAAIAPP
                     YPPDLDNQLAQLAARDVRAVVVHKTLLAPEQFNALRDYFTRIPAYEDEQIVVYSTRPS
                     REPSIPVGSGIGLFNGWAALSESPSAALRGQASAALEVHLRWAVTRSLRGDFDYRLSL
                     VGESGIHVAEQTQHIDPPTSTWQPGALMAGDYQLSLPARLSPGVYRIRLDLRDSDQGV
                     GMLELAHRILSLPEGPGPRQMVVSEEPRVRYGDAIELRAADVSVRGNWLLLWLYWHAL
                     EAPGADTLYFVHVLDGDGNLVAQNDGIHVKYTRPSSMWQADEIIADPIEIPLWNLPTG
                     EYLIAVGLTDTDGVHLTAIDASGQPRPDHRTILETRFAINRPTD"
     gene            17181..18839
                     /locus_tag="AC162G_08235"
     CDS             17181..18839
                     /locus_tag="AC162G_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR5075839.1"
                     /translation="MTRLLIVGLDGATLDLIKPWAAEGKLPALAALMQAGASGPLEST
                     LPPVTSPAWPAFMTGKNPGKHGVFDFLRPSAGAFEMVNASQIDGKLLWEILSDAGRSV
                     GVLNVPITYPPRKVNGYLVPGLLSPDQGVTTYPPDLLKPYRAELGRYRITPDVQYSPA
                     SADAFIADIHDLIDTQLRYALRLMQDRPTDVLMVHFLASDNASHALWRYMDSTHPLHD
                     PALAPKYGDALLKVYQHLDEAIQSLISNLQSPTSKPQTPNPNIIVMSDHGFGPLHKTV
                     NLNVLFLQAGLMHLKPHALTRLRYAMFKRGLTPKGVYRMLERMGLQNITARVSRQTRN
                     QVVSKFLSFEDVDWSRTVAYAMGHVGQVYLNLKGREPEGIVKPSAYHATRQKVVDALK
                     TLHDPQSGRPLVDRIILREEAAWGPYLDRSPDLHLVLDGYRAIAFPLFATEGKVVTPQ
                     IRGDSGCHRLHGIFIGSGPAFASVSIDGARITDLAPTILHILDVPVPSDMDGRVLVKA
                     LAPDLRARAVRTAHAADYIPQQVDFSADEQAEVEERLRALGYLG"
     gene            18839..>20076
                     /locus_tag="AC162G_08240"
     CDS             18839..>20076
                     /locus_tag="AC162G_08240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR5075840.1"
                     /translation="MIDAPFSNLQSRIPQLPTWFWALLIFAVALAPRALALGSYVTPD
                     EPNWVYRTLNFGAALARGDWAATAQTSHPGVTTMWLGSLGIAAQRAVDPAGTADAINW
                     LSKIDRLSPENLEALRRVGVFLDWARLPVIVVNALGLVGVFLLASRLFNRASARLAAL
                     LLALDPFFAGLQGLLHVDGLMTTFSTLSLLSLLLAIRSPQHETHESSSPSLVTRHSLF
                     VVRRSPFFWFGASGAFAGLALLSKSPAIFLVPFGALALLAALVARRLSARDAVIGCTF
                     FFILYSTLFIALYPAAWSDASSALGRMFEGVVYHTATATRPTFFGGQAELDHGIEFYP
                     VTLSYRLSPVVMLGLFLAAIFLFTRNRRDSRTLIFTLLLFAMSFIVFLTPAAKKFDRY
                     VLPAIPPLIIAAAWGIHQLT"
BASE COUNT         3509 a         6871 c         5951 g         3745 t
ORIGIN      
        1 gacgccatcc gcgtcggcct gatctacaag cctggcaagg tggcgccggt cggcgacttc
       61 aagcttctga ctacggctgt agacccgcgc ttcctggaca ccaagaatcg tcctgcactg
      121 gcccagacct ttgaggagct ggcgaccggc gctcgcttca cagtggtagt caatcatctc
      181 aagtccaaag gctccgactg caacgatgtg ggcgacccgg acatcggcga cggccagggc
      241 aactgcaacc agacccgtaa ggccgccgcc caggccctgg tggactggct ggcgaccgac
      301 ccgactggca gcggcgactc ggacttcctg atcatgggcg atctgaactc gtatgccaag
      361 gaagacccga tcgacgccat ccaggcgggc gccgacgata cggccgggac cggcgacgac
      421 tacaccaacc tgatcgccca gtaccagggc acgtacgcct actcatacgt cttcgacggc
      481 cagtccggct acctggacca cgcgctggcc agccccagcc tggctgcgca ggtgacgggt
      541 gccgccgatt ggcacatcaa cgccgacgag cctgacctgt tggactacga caccacgttc
      601 aagccggcgg cccaggaggc gatctacgag ccgaacgcat accgctcgtc ggatcatgat
      661 ccggtggtcg tgggcctggg cctggccctc aacgctacct gcaacggcct gcccgccacc
      721 cttgtcggca cgaccggcga cgatgtgatc aatggcacca gcgggcctga cgttatcgcc
      781 ggcctgggcg ggaacgacat catctacggc ggtaagggcg atgatgtgat ctgcggctat
      841 gccggcgacg acacactcaa cggtgatgat ggaaacgata cgctcatagg cagctatggg
      901 aacgacgtac tcgatggcgg aaaagggaac gacacgttga caggaggcga tgggtctgat
      961 gtgttggatg gccaagatgg tgaggatgtg ctcgatggcg gcagcggtaa tgacacgctg
     1021 atgggtggcg acggcgacga cacgctagcg ggtggcaatg ggaacgatgc gctgaccggc
     1081 agcaacggcg acgatgtgct ggacggcggt gcagacaatg atacgttggt cggcaacaag
     1141 ggggacgact cgctgaccgg cggcacaggc gccgactcgt tcagcggcgg cctgggcact
     1201 gacacgaata tcgacttcaa cgcgagtgag ggggacacga gcgacggaac gtaatctcgc
     1261 cggggtgtga cgcgctgcgc ttgcgcgaca cacgtgtgac ccagccgtca cgcgtaaatc
     1321 aggcgcgggt cacagacccg ctttaagtat ggaaatgcaa acggccgctc cggtgtggga
     1381 gcggccgttt caggtcctgc catctgcttc acatcggctt gcgccttgcc gcccagccga
     1441 cgatctgcga cgccatcgcg ccgtagtcgt aaccggccac cttcgccgtc ttggcgaacc
     1501 cgccgtcgct ggtgatgtcg cagttggcgt tgacgtccac gacgtatggc acaccgtcgc
     1561 gcacgcgtgt gtcgatgcgg ccgtagtcgc gcaagcgcag cgcgcgatac gcggccagcg
     1621 ccgtcgtgcg gatcgcctcg gccaccggct cctctaattc tgccgggcag cgggaaggcg
     1681 tgtgcgtata ctggaaagag gtcggcaccc acttcgagtc gtaatcgacg acccgccggc
     1741 gcgggtcttc aatgtcgccg aagtcgatct cgtacagcgg cagcgcctga ggcgcgccgt
     1801 tgccccagat ggcgacgttg atttcacgcc cggcgatgaa ctcctcggcc agcgcgccga
     1861 cgccgaactt ctccatgagg gtgtcgatct ggcggtgcag tccggccacg tcgtccacca
     1921 ctgaatcggc cgtgatgccg tacgaacagt gctcggccac cggcttgacg atggcgggga
     1981 aggtcgtcca ctcgtctgta tcccgcgtat cggtgaacac gcgccagcgc ggcgtggcaa
     2041 tgccgagacg ctcgagaatc ttcttgaact ccgccttgtt ctgcgtgtgc tgcagcgccc
     2101 acgcgtcgga accggtgtag gtgaagccca tgtcgtccag cgtccgggcg accaggtcgt
     2161 acgcgttcgg cgcgccgtcg atgccctcgc accagttgaa aacgatcgcc tcgcgcggat
     2221 cgtagacgcg cagcggatcg acgatgtccg cccgcacttc caccggctgt accgcgtggc
     2281 cctgattgcg caggccgccg gccatgcgct cgacgtgctg catgcagtcg agcacgtcct
     2341 ccgccgtcca cgtcgagtcg tagttataca acagcagcac gggcggatga gaaccattgc
     2401 cctcgctcat ctcacttacc tccttaccag agctccctga gttccttaag ttcctttttg
     2461 gaaccgaggg aactgaagga acttgagcac atcactcgac acgcttggta tagatcacca
     2521 atccatcgcc gctgcgatag aaatccggca gccgcaccag gcggcgatag ccgcagcgct
     2581 cgtacagcgc ccgcgcggca gcgtacgccg gcgtgtctga cgtctctacc cacatccagc
     2641 caccgccaca cgccctcgcc tgcgcctcga cagcctccat caaggcgcgc ccgacgccgc
     2701 gccgctgcac gtccgggcgc gcggcaatcc agtacaagtc gaacgcgtgc attgccaggt
     2761 cgcgcggccc ccaggaagcg aagcccagca cacgcccgtc ttcacggtac gacagaaaat
     2821 gatagcccga cacttccggg tcgtcgtagt acgcgtcgag catctctgcc accgtggcga
     2881 cttcgcgctc agtgaacacg gccgcctcga tcgcaacctg cacgatctgc tcgcgctcgt
     2941 ccggtgacgt gggctgcacc gagcctgtgg cgagccaggg cgagggtggg attgtttgca
     3001 agaccgccct cacagagcgt cctccttgcc tcgacgcgcc cacgcccagc gggcgatgcg
     3061 cgcgaccatc tgcgggtagt cgtagccagc ggcacgcgcc gctttcggga agccggcgtc
     3121 aggggccagg catgggttag ggttgacctc cagcaccatc ggccggccgt ccttcagccg
     3181 catgtcgacg cggccatagt cccggcagcc catcacccga tacgccatgc gcgccgtctt
     3241 gccgaccctc tcggccagcg cattgtcaat gtcggccggg cagcgcacgt acatcgtctg
     3301 atactccggc gcctgcgggt tccatttggc gtcgaagtga agaaagcgct gatacgggtc
     3361 ggcccagtcg caatagtcaa gctcggcgat aggcagcatg tatcgccggc cgttgcccca
     3421 caggccgaca ttgaactcgc gtccatcgat gaactcctcg accaaggccg actcgcggta
     3481 cgcatccagg acgtactcca cgcgccgccg cagcgataac tcgtcgtgga cgactgagtc
     3541 gcggtcgatg ccgatgctgg cgtcttcggc caccggcttg acgatggccg ggagcggcac
     3601 ccggatcgga tcgtccggct ggctgaagac ctggtagcgc gcggtcggca cgccggctgc
     3661 cctcaacatc tccttggtgc gcgccttatt cagcgagctc gcgagcgtgc ccaccgacgc
     3721 gccggtgaac tggatgccca ggtcggtcat gcgctgggct acctcgccgt cacgatgcga
     3781 gtcgccttgc aggtgctcac acagattgaa caccagcgtg gtctcgacgt cgaattccga
     3841 cgcaattcgg tcgacgtcgc tcaggccgta aaccacgtag gtctgcgcgc gaatctgggc
     3901 gcgattcagg ttttcggcga tggtctgcgc cgtctggatc gtctccagct cggcttccag
     3961 ctcgcgcggc tcgccgcgaa ccgtgcgctc gatggcgctg taggccacca gcacccgccg
     4021 cagcgggcga tggggcgcca caccttcctc gacggccgcc cccgcccgca ccctcccgag
     4081 gacagccggc aatatcagtg aagcctcacc caggcttgcg tccattgtta gatgccctct
     4141 cctccttcag gctgatcgct tcgacgaggc cgagccgccg aagcgctgca tccaaaatgc
     4201 cgttgacgag atcggcgtgc gaccagccgt cggccgccgc catcagcgtc aggtcgctga
     4261 tcggcgtaat gccgggcagc gagttgatct ccagaatgta cggcttgtcg ccttcgagtc
     4321 gattcaagcg gaaatcgacg cgggcgaagt ccagtgtatt cgtcacgcgc atggtctcga
     4381 cggccaggcg aaccagctcg cctgccagcg acccgggcag atcggccggg cattgatagc
     4441 gatagtcgtc tgcccgatcg accttgtatt cggcagtgta aatcggcacc gctcgctccc
     4501 cataaccgga ataatcgacc atcgagatgg ggaacacgtg cgggtcgggc cagttgccga
     4561 ccatgccgac cgtcacgtcg cggccctcga tatactcttc gaccagcgcc ggcccccgat
     4621 aagcgtgtat caaatacgtc acccgatcgc gcacttcggc ttccgtgtgc aacaccgagc
     4681 gttcgtcgat gcccatgcca gtgccttcgc gcgccggctt tgcaaatagc ggaaaccgca
     4741 gggtcgggtc cagcggctcg ccgggcgaac ggaattcctg aaaggcgggc gtcggcaacc
     4801 cgtggaatgc cagcacgcgc ttggtcatcg gcttgtcgag tgtcagcgcc agcgcccgca
     4861 cctgcgagcc ggtatacggc acgccgagca tctccagcag cgctggcgcc tgcgcttcgc
     4921 gcgcgtcgcc tcgaaagcct tcgcaggtgt tgaagcagat gtccaccggc gtcccgcgca
     4981 aacgcgcggg caaggccgca ttcccctcca tgggaaacac ggtatacccg cgcgcttcga
     5041 gcgccgcgtg ataggcggct acgttattct cgtggtccag ctcgtacagg taatcgtgtg
     5101 gcgcgccagg cggcggctcg ggtgcgttcg acgccaggtt gaccagcagc gcaacgcgca
     5161 gcgagcgccc cgagcgcgtg gagtcgcact cctcgaattc tttatctggc tcgctcacct
     5221 gacctcacag tctgacggcg gttacgattc acgcccctcc gccgttgtcg gacggggctt
     5281 gtgcgccctt gagcaccggc ttatgctcgg tgccgatgcc cagcggctgc cacttcgact
     5341 cgtcggcgcg cagccggtgc tggccgccgc cgcgcgtgtg cacgtcctcg aagccttccg
     5401 gctcgatggt catgcgctcg ccttccagaa gcgctgccac accctcctgt cccggctcca
     5461 ggcggcgcgg cgcctgccga tccagccact cgatctcgtg cgggtcgtag tccagcggct
     5521 cggtgtaggt cgtgatgaag ccttcgaagt tgcgaagcac gaccttgccc ggcgcctgcg
     5581 agatgacgta attcggcatg acgggtatct tgccgccgcc gcccggcgcg tcgatcacgt
     5641 acgtcggcac ggcgtagccg ctcgtgtgcc cgcgcaggcc ctcgatgatc tcgatgccct
     5701 tcgatacact ggttctgaag tgccctgcgc ccatgaccag gtcgcactgg tacaagtaat
     5761 acgggcgcac gcgcatcatc accaactcgt gcacaagctg gcgctgaatg tgaatcgaat
     5821 cgttcacgcc ggccagcagc acgctctggt tgcccagcgg cacgcccgcg cgtgacagcc
     5881 gatcgcaggc ttcgtacagt tccggagtga tttcgttcgg gtggttgacg tggatattca
     5941 tccacaccgg atgatacttc gccagcatct ggcagaactc gtcgtcgacg cgctggggca
     6001 ggaataccgg cacgcggctg ccgatacgaa tgatctcgac atgcggaatc tcccgcaggc
     6061 ggctcatcaa gccgtcgagc agcttaggcg ccagcgccag cggatcgccg ccgctcaaca
     6121 gcacgtcgcg cacctgcggc gtccgggcga tgtagtcgat ggcggcgtcc cactccttgc
     6181 ggctgaagtt ctcggtcggt tccccgacga tgcggctgcg cgtgcagtat cgacagtacg
     6241 aggcgcactg cgtcgtgacg aggaacaaca cccggtcggg atagcgatgc accaggcccg
     6301 gcacggggct gtgccggtct tcggcgagcg agtcctccat catcgactcg aacgcgtcca
     6361 gctcgcgcgc ggtggggacg acctgcttgc ggatcgggtc gtggggatcg tccggggcga
     6421 tcaggctggc gaagtacggc gtgatgtcga cgcgaaacag gttcttcgtc gtcagcgcct
     6481 tgatctcgcc ctcggtcagc gtcacaatgc gcgacagctc ctcgaccgtg ttcaggcggt
     6541 tcgacatctg ccaccgccag ttgttccagg tgctgtccgg tacgtcttcg aagcccgccg
     6601 cacgcttacg cggcttttcg agaatgcggt tcgtcatagc gatcctccag ttgtgtcggt
     6661 ctccaacctc agacttcgga agtcttcgcg aagcttccca ccggcgaagc gcctcgaacg
     6721 tcctcacaac ttagacagca cggcgcgcca tgcagacttc cgaagtcctt gaccaactgt
     6781 aacacaactg gacgctcttt gtctaacccg atgcattgta cagcaattca cgcagcgttg
     6841 agtgtaaaca tagtgaaaac ttttcggtga aatgctggtg aaaaagatcg ggccgcgatc
     6901 gattgggcac gactcgatcg cggccgggtc agacgatgga cgaaagacga gggacagacg
     6961 agggcgcgaa gagagaaggc gattactcgg gaacggacaa catataccca aacccgcgca
     7021 ccgagcgcag gtattctccc gtctgcgggt tccatctcat cttgcggcga atgaggcgca
     7081 cgtgtgcctt cagcagctcc tgtgcttcgg ccttgctgcc gtcgtagccc cacacctgtc
     7141 gcagcagctc gcgaaaaccc agcacctcgt tcggcctttg cgccagcgtc acgagaatct
     7201 ggtattgcat cggcggcaac tgcacccact tgcgccggcg gaacttggcc cgcttgcgcg
     7261 ccaggtcgat cgtcaagtcg cccaggcgca gccactcggc cgagcggcga gaagagcgga
     7321 gcaaagtcag cgctgccccg acccgactgc gatctacctt ggtgggctta cgcccgccgt
     7381 tcttgatggg cacgatcacc ggccagcggc caaccgaaac gaacatcctc gtcaacgcct
     7441 gaaactgtcg atcgagaaac gcgcggtcga ccagcatgag ctcgagcgca gattcatgat
     7501 gcagcacctt cgcagccgcc gatacgttcg cggcgatgag acacaccacg ccatcttcgg
     7561 cgagcgcctg cgcccaccgc gcagcgacaa tctcctcggc caagatcatc acgcgctgcc
     7621 tgcggcgctt agccccatcg gccagttcag tcatgatctc tccttagttt cattataatc
     7681 cgtttcgatg atcgatcaac aagagcagcg cccggcgcgg cagccgcctg cccttgcgtc
     7741 gagacacgag caaggtacaa taccggttga ctccctcgcg catccatcgg ccatgcccac
     7801 acctgtgcag cacctcgtca tcgctgacgc cattctcgcg aagtcgagcc ttcccgagca
     7861 cgcgcgcgcc tttctcaacg cgcagcgcgc cgagttcctg ttcggcaacg tcgcgcccga
     7921 cgtccagacc gtctccggcc aaccacgcgc ggcgacgcat ttcttcaacg tgccgctgga
     7981 ctccagccgg ccggcgcatg aggtgatgtt cgaagcccat acccacttga gcgagccggg
     8041 ccggctgccg gccgggcagg ctgccttcct tgccggttac atcatgcatt tgctgctcga
     8101 cgtgatgtgg gtgcgcgaca tcttccagcc catattcggg cccgatgccg gctggagcac
     8161 ctttcacgac cggctcttcc tgcacaacgt gttgcgcgcc tggtgcgacc gccgcgatca
     8221 ggcacacctg gcaggaaacg ccggcgcatc cctggcgacc gtcacgccaa accactggct
     8281 gccattcacc ggcgacagat acctgcgcca atggcgtgac gcgctcgtcg agcagctcgc
     8341 ccctggcgct accattcgca ccgtccaggt attcgcagag cgcggccaga tcgaccccct
     8401 ggcgtttgag cgcgtgctgg aatccgagga cgaattgaac gaccgcatat tcagccaggc
     8461 gccacgccgc gtcattgacg atttctacac gcgggggctg tccgaatcgt gtgcgctcct
     8521 caacgactat ctggcccgcg tataagatgg ttcctgtcgg gaaacgccat agagcggcat
     8581 cccgatgcgg ttcgaataag gctggcatag ggatgccagc ctactttctc gacaagaacc
     8641 aggatagacg gacgcctgcg caagggcaag ccggttgcag acggagcgta cgtggtacaa
     8701 tcgcgtccta gttgcggaac cgaactggag tctggcgaat ttccggagtc gagcctttag
     8761 gcggcatatt tggcggccca caccggctaa agcctcgact ccagcgccag atgtggcggt
     8821 cgccaaactc cagaaccgaa taacgcgcgc tggcagctct ggtcgtgacc cggccatgac
     8881 gcgcggctga cttccctttt caaggagatc ccttccatga agatactcgt caccggcgga
     8941 gccggctacg tcggcagcca ttgtgtcgaa gagctgtgcg aggcgggcga agacgtcgtc
     9001 gtcttcgata atctttacta cggccatcgc gaggccgtcc accccaaagc gaaatttgtc
     9061 gaaggcgatc tgaaggatgc cgacgccctc aaacggctgt tcgacgcaca gccgtgtgac
     9121 gcggtgatgc actttgcgtc gcatacgctc gtcggcgagt cgatggagaa gccgctgctg
     9181 tacctgggtg acaacatcac caacgcgctg aatctattcc gcgcgatgat cgaccatggc
     9241 gtcaggcgct tcatcctgtc gtcgacggcc aacctgttcg acaagccgga gcgcataccc
     9301 atcgacgagg acgagcgcat cgtgccgggc agcccatacg gcgaaagcaa gaacatcatc
     9361 gagcggcagt tgtactggct ggacaaaatc tacggcatcc gctacgcctg cctgcgctac
     9421 ttcaatgcgt cgggggcatc gccgaccggc ctgcgcggcg aggatcatga gcctgaaacc
     9481 catttgatcc cgatcaccct ccaggtcgcg ctcggccagc gcgagaaatt gattgtcttc
     9541 ggtaatgact atcccacgcc ggacggcacg tgtgtacgcg actacgtcca tgtcaacgac
     9601 ctggcgcagg cgcacatcct ggcgctgcag gcactcgacc agggcagccg cacgtacaac
     9661 ctcggcaacg gcaacgggta ctcgaacctg gaggtgctcc agaccgccga agccgtgacc
     9721 ggcaggaaga tcgcctggga gttcgggccg cgccggcgcg gcgacccggc cgtcctcgtc
     9781 gccagcagcg agcgcatcca gcgcgagctg ggctggaagc cgcgctacgg ctcgctgcgg
     9841 gacatcatct ccacggcctg ggaatggcac cgggcgcacc cgaacgggta cgatggcaaa
     9901 tagagggcgt gctgtatgca tatcgtcatc aatggctggt tcctcaacca accgaacacc
     9961 ggcagcgggc aatacctgcg acagctcatc acgcgcctgc ctcgactcgc gccgcagcac
    10021 gagttcatcc tcgtcgtccc cgaccgccgc tcgttcaggg tcttcgatct ccacacagac
    10081 gccaatctcc aatctctaat ctccaacctc caacctccaa cctccgactt ccgcaaactc
    10141 ttgttcgagc agtccattat cccgcgcgcc gcacgcgccc tccgcgccga cctcattcac
    10201 gtcccgtact gggcgccgcc gctccgtgca agcgtgccaa tcgtcgtcac gatccacgat
    10261 gtgatcccgc tcgtcctgaa agaatatcgc ggtgggccgc tggggcgcct gtacaccggg
    10321 ctggtgaccg ccgctgcgcg cggcgccgcg ctcaccctca ccgattccga ggcatcaaaa
    10381 cgggacatcc ttcaacacct tggcgtctca gaatcgcgtg ttcggacgat ctacttggcc
    10441 gccgatccga agttcacacc ccggcgcgat ccggtcgacc acgccgccgt ccgccgtaaa
    10501 tacgatttgc ccgacaacta cgtgttgtat ctgagcggct tcgacccgcg aaagaacgtc
    10561 gaggcggcgc tgcaagtgta cacctggggg caggacgcca tcggccacag ccacccgctc
    10621 gtcgtcgcag ggcggctgcc ggaaggcggc gacggcttct tcacagatcc gcgcgagatc
    10681 gtgaagcgca tcgaaatcga agacgtcgtg cagtttatcg gcgaagttga cgaggaagat
    10741 aaggtcgctt tgtatcaggg cgcgtcggca ttcctgtacc catcccgccg cgaaggcttc
    10801 gggctgccgg cattggaggc gctggcctgc ggcgtgcccg tgatcgggtc ggaggcgtca
    10861 tcgatccctg agatcgtcgg cgacgccggg atgctcgtca gccccgacga cgcgcgcgcc
    10921 atggccggcg cgctgatcgc cgtcgtgacc gagccgccgc tgcgcgaggc gctcagcgag
    10981 cgggcgctta aacaagcagc aagattctcg tgggacaaga ctgcccgcga gacggttgag
    11041 gcgtacgaat cggttatgaa agcgtagcac gcatgcagtg acgacttcat ccttaatctc
    11101 gttagggaaa cgacaacgcg aaattcttcg tcccgcgctc ttcgatgttg cgctcctctt
    11161 tgcggcgttt ctactctaca ctctaactct aactccctcc cttctccccg ccgacagcgg
    11221 cgaattccaa ctggtcgcgt ggaaactcgg catcgcgcat ccgcccggtt atccgctgta
    11281 catcgtcgtc gggcatctat tctcgcgctt cttcgcgtca cccgccttcg cgctcaacgt
    11341 gctcagcgcg atcctggctt ccatcacgct cgtcctcgtc agccgcgccg tccgcgcagc
    11401 cacgggttcg acgctgggcg ggcttgccgc tgccgtgata ctcgccacgg ccacgacgtt
    11461 ctgggcgcaa gcgacgacgg cgaacatccg catgccgacc gcgctgttta ccgccgcatg
    11521 cgtctacgtc ctcgtcatgc atcagcaggc cgtcgcagaa gcccgcaaca ctgcagccgc
    11581 agcgcccccg cgccttcgag ccggctcccc cgatcgatac ctgattctgt ttgccctgct
    11641 gttctccctc ggcctcagcc accatgtatc gctcatcttc cccggcattt tcttcgtcac
    11701 cttcgtgctg ctcgtcgatc cctcgatctt ccggcagcct cgcagatgga tccggcccgg
    11761 tatcgctttt ctgctcggcc tgctcccact gctctatctg cctgcccggg ggctggccgg
    11821 cgggacgctc gccaacggcg aatcgggagc ggaattggcg cagccagaca gatttctcga
    11881 ctatgtcctg gcgcgaggat tcgagggtga cttcttttac ttcgtcacgg agcggcccga
    11941 cctgctcgac gatcgactgg cgctgctgcc gacgctcttc gacttccaat tcaacctcgg
    12001 tgcgctgatg ctcggcttgc tcggcgccgt cgtgctcgcg cggcgaaact ggcggctgct
    12061 ggtcatgctg ctcggcggca tcgccgcgca taccttcgtc acgctcgcct atcgcgcccc
    12121 gcagacggtc gaatacttga tgccagctta cgtgctcttc gccattctga tcggctgcag
    12181 cgttggacaa gtgttaacca cggagacacg gacgcttggc gcacaaaggt tttctttgtg
    12241 gttcaatctc gttctctttg tgatggcgct gatcgccggc cttgcacgtg ggctcgataa
    12301 cctgccctca tacgactggc tctcgcgaaa tggggatacg cgcgcgtatg ccgaatccct
    12361 gctcgaagat gcgccgcccg acgcgatcat cctgtccaac tggcactggg ccaacccgat
    12421 gtggtatctg cagcaggtcg agagattgcg cgccgacgtc gacgtggtct acgtctaccc
    12481 gcgcggggaa ccactcgcca tcacgtggct gaaggagatc gactcctccg tcacgaccgg
    12541 ccgcccggtg atcgtcgaca tgtacttcca cgaggagttt ctcgcgtcgc cgtatttctt
    12601 cgagccgatc agccaggaag ccttcctggt gcgccagtcc tacctctcca atcttcccca
    12661 gggctacact ccctatgaag ccgaattcgg agggaaattc aagctcctcg gctttcgcct
    12721 gctggacgag cgaacaacac ccggcgaacc atacatggtg aacctcgcct ggcgggtcga
    12781 gtcgcagccc gagcgggact actcgttctt cgttcacctc gtcgattcat ccggccgggt
    12841 cgtcggtcag gccgaccgca cgctgccgac gatgcgttac aagcctggcg acgtgatcgt
    12901 cgaacggttc ttcgtcgccc cattgcccgg cgttctgccc gacgagtaca ctttggctac
    12961 cggcatctac accgtggaaa atacggaaat tgtcgaattg ggtgggcaag cccaattcgc
    13021 ccgcacgatc gtcgagccgc ccgttcgatt agcggatcag cccacttctg ctgttgccct
    13081 ttcgcacggc atctacttcc tcaacccgct cccctaccct cgggcgagcg cactctatcc
    13141 gggcagccag ctcgtcctcg acctgcgctt cctcgcgtcg cggccgctca cgcgtgatat
    13201 cgtcgtctcg gtgcagatga tcggcgaagg ctacacctgg aagacgaccg ccgactccgt
    13261 gcctgcgctg ggcgcgatcc ccacgctcaa gtggatcgcc ggctcccagg tcgccgatag
    13321 acacgtcgtc gatatcccgc ctgatacgcc gcccggcccg gcaaccgtct cggtcatcct
    13381 gtacgatcac ttcacacagc agccgctggc cctgctcgac ccgcaactga ttcaacaaga
    13441 ccggtcgatc ccgctcggcg cgtggaccat tttgccgccg tagcaactct tgtacgtcca
    13501 tactccctgt ccagttcatg ctataatttc aacatcacgg ccctccaccg ggccggtttt
    13561 ttcgccgata ctcccgaaat aatggcagac cacctcgacc tctctatcat catacccgcg
    13621 cgcaatgaag cggaatcgct gcctagcctg cacgccgaga tcggcgcggc gctggcgccg
    13681 ctgcacaagt cgtacgagat catcgtcgtc gacgacggca gcaccgacga cacgttcgcc
    13741 gcgctcaggc ggctcgccga gtgcgatccg cacgtggtcg ccgtacggct acggcgcagc
    13801 ttcggacaga cggccgcctt ggttgcgggt ttcgatctgg cgtgcggcga tgtggtcgtc
    13861 accatggacg ccgacggcca gaacgatccg gccgatgtcc ccaagctcct ggataagatc
    13921 gatgagggct atgatatcgt atccggctgg cgggtgaatc gcaaagagcc gttcatcacg
    13981 cggcgcatcc cgtcgatgat tgccaatcgc atcctgacag cgcgcactgg cgtccgcctg
    14041 cacgattcgg gctgctcact caaggcgtac cgcagcgagg tcgtcaggca tatccggctg
    14101 tacggcgaaa tgcaccgctt cattccggcg ctcgcctcga gcatgggcgt gacggttgcc
    14161 gaggtacccg tcaacgaccg cgcgcgaaaa cacggcaaga gcaaatacgg cctctcgcgc
    14221 acctttcgcg tgatcctcga tctcttcacg ctcaacttcc tgctcggctt ccagggcaag
    14281 cccatgcaac tgttcggctg tgccgggttg atcaccggcg cgatcggctt cgctatcctg
    14341 gcctacctca cctttctgaa aatcgcgtac ggcgagcagt tgagcaatcg acccgtcttg
    14401 tggctggggg tgctactggg tatcgtcggc gtgcaattcc tgttcttcgg actgattgcg
    14461 gaaatgctga cccgcactta ccacgaatcg caaggcaagt cgatctacgt catccgcgag
    14521 atcgtcggac gcgacgccat ccacgaacct ccacatgaaa gagttggcga gccggcgaac
    14581 tcgccacacg actcgctgac cacctgactc attccccatg cggcaatgcg accttcgatt
    14641 cggcggcgac ttcgccgcga tctcttcgtc ctgctgttct tcactctgct gacgctcgtt
    14701 ctgacctggc cgctcgtcgc acgcatcggc gatgcctacg ccggcaataa cgaggacctg
    14761 tgggtgtttc aatgggacaa ctggtggacc cgcgtcgccc tgcaacaggg tctcgatccg
    14821 ctgtacacac cctaccagtt ctatccaggc ggcgtcagcc tggcggccca ctcgctcagc
    14881 ttctacaact cgctgatctg gatcccactg gcggcgctgt tcggcgacat cgccgcctac
    14941 aacatcacga ttctgctgac gttcatcctc agcggctaca tgatgttcaa gctggccgaa
    15001 tacctgctgc ccgacgcgcg tccgccgctg ccggcgctgc tggctggcgt cgtgttcgcc
    15061 ttcgcgccat atcacttctc ccagtcgctc ggacacgtga gcctggcgag tgtgcagtgg
    15121 tttcccctgc tggcgctgtt catcctgaaa acggcgcgcg agcgcgcgtg gcgtaacgtc
    15181 gtcgccatcg gcctgctggc gttgctgatc gccgccactc gcctgcaatt cctgatcctg
    15241 ggcggcgtcg tgttcgcgtt gttcgtcggc gtcgactggc tggtctcgcg gcacgagtgg
    15301 agccggggcg cgctcgcgcg gctgattgcg ggcgcggcgg ccgggcttac gctgtgcctg
    15361 ccgctgcttg tgcccgccgc ccggctgtac acgcaggccg cctcccccga cgaattgatc
    15421 gccgacgagg acgagtgggg gcagaccgat ctgctggcct acgccgtgcc gatgacctat
    15481 catccgatct tcggcgcggc tgtccagccg ctgtaccagc gctttgtcaa gaaccaggcc
    15541 tggatgccgt tcctgggcta cacggcggtc gcgctagcgg cctgggggat cgcgcgcctg
    15601 cgccgacgcg cctggccgtg ggtcgccatc ggtgcgttcc tgttcgtcat ggcgctcggc
    15661 cccacgctgc gcgtgaacgg catggtgtac gaggatattc gtttgccgta cgcgctcatc
    15721 ggcgattggt ttccgctcaa cacgctgcgc tcgcccgatc gctacaactt gttgatgccg
    15781 atggcccttg ccgcgctggc gggggcgggc gcggatggcg tcctgcggcg ttgggccagc
    15841 cgccgcggaa cgatctgggt cggcctgctc ggcacgctga tcgtcttcga gtatctcggc
    15901 ctgccgtacc caacgatcga gccgttgccc acctcgccgt acttcgacgc gctggccgcc
    15961 gatcccgagc catacgccat tctcgacatc ccgctggagc ggtccgacac caagcgttac
    16021 ctctattacc agacgcggca cggcaaaccc atcgtcgaag gccgcgtggc gcgcgtgccg
    16081 gcggaagcct acgcgttgtt cgatgcgatc cccttgctcg acggctggcg tgccgccatc
    16141 gccccgcctt acccgcctga cctggacaac cagttggcgc aattagccgc ccgcgacgtg
    16201 cgcgcggtcg tcgtccacaa gactctgctg gcgccggagc agttcaacgc gctgcgcgat
    16261 tacttcaccc gcataccggc ctacgaagat gagcagatag tggtatactc gacgcgtcca
    16321 tcgcgcgagc caagcatacc tgtcggcagc ggcattgggt tgttcaacgg ctgggcggca
    16381 ttgtcggagt ccccctccgc tgcgctacga gggcaggcga gcgctgcgct cgaagtgcac
    16441 ttgcgctggg cagtgacccg gtcgcttcgg ggcgatttcg actatcgcct gtcgctcgtc
    16501 ggcgaaagcg gcatccatgt agccgaacag acgcaacaca ttgatccgcc gacgtccacc
    16561 tggcaaccgg gcgcgctgat ggccggcgac tatcaactga gcctgcctgc ccgtctatca
    16621 cctggtgtct accggatcag actcgatcta cgtgacagtg atcagggcgt gggcatgctt
    16681 gagctggcac atcgcattct cagcctgccc gaaggccccg gcccgcgaca aatggtcgtc
    16741 agcgaggagc cgcgcgtgcg atacggcgat gcgatcgaat tgcgcgccgc cgacgtgagt
    16801 gtgcgcggca actggctcct gttgtggttg tactggcatg ccctcgaagc gcccggcgcg
    16861 gacacgctgt acttcgttca cgtgctagac ggtgacggca acctggtcgc gcaaaacgac
    16921 ggcatccacg tgaagtacac acgccccagc tcgatgtggc aggcagacga gatcatcgcg
    16981 gacccgatcg agatcccatt gtggaacctt cccactggag aatacctgat cgccgtcggt
    17041 ctgactgaca cggacggcgt gcacctgacc gcgattgacg cgagcggaca gccacggccg
    17101 gaccatcgca ccatccttga aacccgattc gccatcaacc gaccaaccga ctaaccaacc
    17161 atccaacact ccgacccatc atgacccgtc tactcatcgt cggcctcgac ggggccacgc
    17221 tcgatctgat caaaccctgg gcggctgaag gcaagctgcc cgcgctcgcc gcgctgatgc
    17281 aagccggtgc gagcggcccg ctcgaatcga cgctgccgcc ggttacctcg cctgcctggc
    17341 ccgccttcat gacaggcaag aaccccggca agcacggcgt gttcgacttc ctccgcccca
    17401 gcgctggggc gttcgagatg gtcaatgcct cgcagatcga cggcaagctg ctgtgggaga
    17461 tcctcagcga cgcgggccgc tcggtgggtg tgctcaacgt gccgatcacc tacccaccgc
    17521 gcaaagtcaa cggctacctc gtgcccggcc tgctctcacc cgatcagggc gtcaccacct
    17581 acccacccga ccttctcaag ccgtatcgcg ccgaactcgg acgctaccgc atcacgcccg
    17641 atgtgcagta cagcccggcc agcgccgacg cgttcatcgc cgacatccac gacctgatcg
    17701 acacgcagtt acgctacgcg ctgcggctga tgcaggatcg cccgaccgac gtgctgatgg
    17761 tgcacttcct ggcgagcgac aatgcctcgc acgcgttgtg gcgatacatg gattcgacgc
    17821 acccgctgca cgatccggcg ctcgcgccga agtacggcga cgcactgctc aaggtctatc
    17881 aacatctcga cgaagccatc caatctctaa tctccaatct ccaatctcca acttccaaac
    17941 cccaaacccc aaaccccaat atcatcgtaa tgagcgacca cggattcggc ccgctgcaca
    18001 agaccgtcaa cctcaacgtg ctcttcctgc aggctggatt gatgcatctc aagccacacg
    18061 cactcacgcg cctgcgctac gcgatgttca agcgcggcct cacgccgaag ggcgtatatc
    18121 gaatgttgga gcgcatgggc ctgcaaaaca tcaccgcgcg cgtcagccgg cagacgcgca
    18181 accaggtggt cagtaaattc ctctcgttcg aggacgtgga ctggtcgcgc acggtcgcct
    18241 acgcgatggg acacgtggga caggtgtacc tcaacctgaa gggaagggag ccagagggca
    18301 tcgtcaagcc atccgcgtat cacgcgacgc gccagaaagt cgtcgacgcg ctgaagacgc
    18361 tgcacgatcc gcaaagtggc cgcccgctgg tcgatcgcat catcctacgc gaagaggccg
    18421 cgtgggggcc gtatctcgat cgctcgcccg acctgcacct cgtactcgac ggctaccgcg
    18481 ctatcgcctt cccgctcttc gctaccgagg gcaaagtcgt cacgccgcag attcgcggcg
    18541 attccggctg ccatcgcctg catggcattt tcatcggcag tggcccggcg ttcgccagcg
    18601 tatcgatcga cggcgcgcgc atcaccgatc tcgcgccgac gatcctgcac atcctggatg
    18661 tgccggtgcc gtcggatatg gatggacgcg tcctggtcaa ggcgctcgcg ccggacctgc
    18721 gcgcgcgcgc cgtccggacg gcgcacgccg ccgattacat accgcagcag gtcgacttca
    18781 gcgccgacga gcaggccgaa gtggaagaac gccttcgcgc actcggctac ctgggctgat
    18841 gatcgacgct ccgttctcca atctccaatc tcggatcccc caacttccga cctggttctg
    18901 ggcgctgctg atctttgcag tcgcgctcgc cccccgcgcg ctggcgctcg gcagctacgt
    18961 cacgcccgat gagccgaact gggtgtatcg cacactcaac ttcggcgcgg cccttgcgcg
    19021 cggcgactgg gcggccaccg cgcagacgag ccaccccggc gtgacgacga tgtggctagg
    19081 gtcgctcggc atcgccgcgc agcgcgccgt cgatcccgcc ggcacagccg acgctatcaa
    19141 ctggctgtcc aagatcgacc gtctctcgcc ggagaacctc gaagcgctca ggcgtgtcgg
    19201 tgtcttcctc gactgggcgc ggttgccggt catcgtcgtc aacgcgctgg ggctcgtggg
    19261 cgtgttcctg ctggcgagcc gcctcttcaa ccgtgccagc gcgcggctgg ccgccttgct
    19321 gctggcgctc gatccgttct tcgccggatt gcaagggttg ctccatgtcg acggattgat
    19381 gaccacattt tcaaccctgt cgctgctgag tctgctgctc gccattcgca gcccgcagca
    19441 tgaaacccat gagtcgtctt cgccgtcact cgtcacgcgt cattccttat tcgtcgttcg
    19501 ccgttcgcca tttttctggt ttggcgcatc cggcgcgttc gccgggctgg cgctcctcag
    19561 caaatcgcct gcgatcttcc tggtgccgtt cggcgcgttg gccctcctgg ccgccctcgt
    19621 cgccaggcgg ctatccgcgc gcgacgccgt catcggctgc acgtttttct tcattctgta
    19681 ttcgaccttg ttcattgccc tttaccccgc cgcgtggagc gatgcctcgt cggcgctcgg
    19741 ccggatgttt gaaggcgtgg tctaccatac cgccacggcc acgcgtccga cgtttttcgg
    19801 cggccaggcc gaactcgatc acgggatcga gttttatccg gttaccctca gttaccggct
    19861 gtcgcccgtc gtcatgctcg gcctcttcct tgccgcgatc tttctgttca cccgaaaccg
    19921 acgcgactcc cgcactctta tcttcacact actccttttc gccatgagct tcatcgtctt
    19981 tctcacgccc gccgccaaga agtttgatcg ctatgtgctg cccgccatcc cgccgctcat
    20041 catcgccgcc gcctggggca tccaccaact caccga
//
LOCUS       DBNHXB010000204         7440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_717262, whole genome shotgun sequence.
ACCESSION   DBNHXB010000204 DBNHXB010000000
VERSION     DBNHXB010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_717262"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..623
                     /locus_tag="AC162G_08245"
     CDS             180..623
                     /locus_tag="AC162G_08245"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5075841.1"
                     /translation="MQIDDSFVVHAPPEKVWELFFDLERMGQCVPGIESIERVDDVTY
                     RGKIKIKVGPVAAAFNGTAALTEVDPPRRVVASLEGDDRSIASFVKATFTSTLSSVEG
                     GTQVAYRMEVNLRGRLAQFGTAVINATAKKVTAEFVGNVKAQLES"
     gene            613..1473
                     /locus_tag="AC162G_08250"
     CDS             613..1473
                     /locus_tag="AC162G_08250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR5075842.1"
                     /translation="MKVDFLEPTTLDEAVALLSEYADGAKVIAGGTALVLMLQQRLVA
                     PAALVSLGRIPGWDFIRREADGLHVGALTRLRAAERSNFVREFCPALAHAFGVVGNVR
                     VRHQATVGGNLAEADYASDPPAMLLALDARVNAIGPNGKRQIPLSDFFLGFYTTALAS
                     NEILSEVVIPPLPASCRAVYLKYTSRSSEDRPCVGVAAVADIDESEHCRDLRVAVGAA
                     VETPQRVPKAEAMAMGESLADDLIDAIADEYARALDPLSDVRGSAWYRREMIRVFVRR
                     SLKEVRDGYR"
     gene            1460..3733
                     /locus_tag="AC162G_08255"
     CDS             1460..3733
                     /locus_tag="AC162G_08255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR5075843.1"
                     /translation="MAIGESVPMLDAVARVTGAVEYMVNLRLPGMLVGKIVRSAAPHA
                     RLLKVDAGQAERAPGVVAVLTRADLGPSALYGAAIKDQSAVAIDRVRFVGEPVAAVAA
                     ESLEAAEEAALLVDVEYDELPAVFDALEAIRPGAPMLHDQFPNNIFKHAKLRHGDVEA
                     AFAEADEVFEDTFTSPPAQQTSLEPHAVAAQWDGDRLTVWTASQAPYMVRNALAGIFG
                     LAPDAVRVIVPPVGGGYGGKGHIRIEPIAAALARKTGGRPVKIVLSRAEEFVTVTKHA
                     ATITLKTGVRRDGTFAARQVTIYWNGGAYADASPSLVLGGMVRSVGPYRIPAVRVDSY
                     GVYTNLPPAGAYRGAMSSQTAWAYESQMDIIARKMGWDPLEFRSKNLLRSGERFATGE
                     TLHDVHFAECLEAAVRGLGPHPPPPSPLPPLPIQEIGRGGRGGEAGEGGEVRRGRGCA
                     VMMKSTVARSKSQAKIRFTADEVATLFTSTVEMGQGAHTALAQITADALGVPFGAVRV
                     VGPDTAITPFDSTTSASRSTSMMGSAVRDAAEELKKKLVALAAPILETPADTLKAERG
                     RIVSATESISYADVLRRNDLDALEASGEYATAFGIDPETGQGVSTPHWHQGAGAAEVE
                     VDVETGRVTLVRYSAASFAGRVVNPRLAQLQNDGNVIYGLGPALLEEIVFDGGQVTNP
                     NLSDYMIPSFRDAPPELVSTCLESEGGELHGIGEMTLPPVAPAIANAVEDAVGVRIRD
                     LPITAEKVLRALRGESV"
     gene            3730..4188
                     /locus_tag="AC162G_08260"
     CDS             3730..4188
                     /locus_tag="AC162G_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5075844.1"
                     /translation="MTVLRVNGVDREVDAGLRTLLAVLRDDLGLGGAKEGCGIGMCGA
                     CTVLVEGEPISSCLMLAVQARGRDIVTIEGISREGELHRVQRAFVEYGAFQCAYCTPG
                     FVLSAIALLNENPSPDDDEVRDYLAGNLCRCGSYSNIARAVIAAGWLKPA"
     gene            4202..4864
                     /locus_tag="AC162G_08265"
     CDS             4202..4864
                     /locus_tag="AC162G_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR5075845.1"
                     /translation="MRSSLITPIAVFLLSLVGPAIVLGLPLVLGWSGDTLSSHLYIFG
                     TLGQALMDLLAIVMLARSNGKGLGRKHEHIASLGAGLACAAALAGVKMAASGRLVFME
                     GVPAFTQSLALAGPWNLISATAAVLAYGPGEALYVVYFVTAFDAAWEYQRRLVSRGVI
                     ITTLLWGLSHGWNVFFLGWRALPNALLMVGIGLIIGLLFKRTRSPWGSMVFWSLANGT
                     SA"
     gene            5070..6311
                     /locus_tag="AC162G_08270"
     CDS             5070..6311
                     /locus_tag="AC162G_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024776.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5075846.1"
                     /translation="MHSTKRSDQAHARQRRIAVRLYQAGHAVKSIVQKLKRSRSWVYK
                     WIAYRLQHPWTRFRSGSRAPHHHPNQTPRVVTRRVLRLRQRLVRHTSPAVRFAGVGAR
                     TIQREYRKRYARPPSLSALGRILKRNHCVTQAKKRKRYRPHPSAEYPNAVQATDIITR
                     WIQGGTVVQAFHTIDVYSNDVASTIHAHKAAGDARQHLLETWKTLGIPDLAQFDNESA
                     FSGGRYARRISQVVRLCLYFGIPVLFTPIDEADYNWPAETFNALWAQQFWNRHHFSRR
                     GDVSRVQRSFLDWYRTDYVAPRQSDTPARLRRGRRIRRLPARWADQVPDPLPIYVGQI
                     QAVRRVDAAGSVRFLNEPIRIGKRYAGRYVWLSLDTGHQQLTIWYQPCAEADWRQLMV
                     RDYPLEEAVLPVPKKFARLHM"
     gene            complement(6628..>7440)
                     /locus_tag="AC162G_08275"
     CDS             complement(6628..>7440)
                     /locus_tag="AC162G_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5075847.1"
                     /translation="VEPITFLGTVMALTGNYARAKELVGEGREKARAVGDEWFAAMCL
                     SLQGNIAMLIGEYKIAHERLQSAVAEWRAIGDPRFTAFGLNFLGQIALTLGRYDVART
                     ALEESVALNISVGARWNLGHAYQGLGAVAQAQGEHQRAVDMFRKGVDTFTELGGRFYA
                     AQGLAEMGRSVFALGNDTEAERVWRESLRIAIEIRGMPVAVEALVGLASLQAKKGDME
                     PALELLFIVLNHPASSEETKNRAGHLRAEWEAQLTRQQVEAAQAQARSKRFD"
BASE COUNT         1328 a         2317 c         2347 g         1448 t
ORIGIN      
        1 gctcgagcct gaatcgatcc tgtggcttga gccgggcgac acgtatacct tcgaagcagg
       61 cgcgaatgcg ctcgcgctag tgctttgtcg cgcgcctgcg cacaccgtct cccccccgca
      121 tgcgggaggt accccttctg tctccccacc gcatgcgggg gggactaagg gggggccaga
      181 tgcaaatcga tgactctttc gtggttcacg cgccccctga aaaggtgtgg gaacttttct
      241 tcgaccttga gcgtatgggc caatgcgtac ccgggattga atccatcgag cgcgtggatg
      301 acgtgaccta tcgcggcaag atcaaaatca aagtcgggcc ggtcgccgct gcgttcaacg
      361 gaacggcggc gctcaccgag gtcgatccac ctcgccgcgt ggtcgcctcg ctcgaaggcg
      421 acgaccggtc gattgcctct ttcgtgaagg cgacctttac ttcgacgctg tcctcggttg
      481 agggcggaac ccaggtcgcc tatcgaatgg aggtgaacct gcgcggccgg ctggcgcagt
      541 tcgggacggc ggtgatcaac gcgacggcga aaaaagtgac tgccgagttt gtcggcaacg
      601 tgaaggccca acttgaaagt tgactttctc gaacccacta cgctcgacga agcggtggcc
      661 ctcctctcgg agtacgctga tggcgcaaag gtcatcgcag gcgggacggc gctggtgctc
      721 atgctccagc aacgcctcgt cgcgccggca gcgctggtca gtctggggcg catccccggc
      781 tgggacttta tccgccgcga ggccgacggg ttgcacgtcg gcgcgttgac ccgtctgcgc
      841 gcggccgaac gctcgaactt cgtgcgcgaa ttctgcccgg cgctggcgca tgccttcggc
      901 gtcgtcggca acgtgcgggt gcgccatcag gccaccgtcg gcggcaatct ggccgaggcc
      961 gactatgcct ccgatccgcc ggccatgctc ctcgccctgg atgctcgcgt caatgcaatc
     1021 gggccaaacg gcaagcggca gattcccctg tccgatttct tcttgggttt ctacacgact
     1081 gcgctggctt ccaatgagat cctgagcgag gtggtcatcc cgccgcttcc ggcctcatgc
     1141 cgcgccgttt acctgaagta cacatcccgt tcttcagaag atcgcccctg cgtaggtgtg
     1201 gcggcggttg cggacatcga cgagtcggaa cactgtcgcg atctgcgcgt ggctgtcggc
     1261 gctgcggtcg agacacccca gcgcgtgcca aaggccgagg cgatggcgat gggcgaatct
     1321 cttgccgacg atctgatcga cgccatcgcc gacgagtacg cgcgcgcgct cgatccactc
     1381 tccgatgtgc gcggctcggc ctggtaccgc cgcgagatga tccgggtctt cgtgcggcgc
     1441 tcgctgaagg aggtgcgcga tggctatcgg tgagtctgtc cccatgctgg acgccgtcgc
     1501 gcgggtgacc ggcgctgtcg agtacatggt caacttgcgc ctgcccggca tgctcgtcgg
     1561 caaaatcgtg cgcagcgcgg cgccccacgc ccggctgctc aaggtcgacg ccggccaggc
     1621 ggagagagca cccggcgtcg tggccgtgct cacgcgcgcg gaccttggac cttcagcgct
     1681 ctacggcgcg gcgatcaaag atcagagtgc cgtggcgatc gatcgggtgc gattcgttgg
     1741 agagccggtg gcggccgtag cggctgagag tctggaagcc gcagaggaag cggcgttgct
     1801 cgtcgacgtc gaatacgacg agctgccggc cgtgttcgat gcgctggagg cgatccggcc
     1861 cggcgcgccc atgctgcacg atcaattccc gaacaacatc ttcaagcacg ccaaattgcg
     1921 gcacggcgat gtagaagccg ctttcgccga agccgacgaa gtcttcgagg acacgttcac
     1981 cagcccgccg gcgcagcaga cctcgctgga gccgcatgct gttgcggcgc agtgggacgg
     2041 cgacaggttg accgtgtgga ccgccagcca ggcgccctat atggtgcgta atgctctggc
     2101 gggaatcttc ggtctggcgc cggacgcggt gcgggtcatc gtcccacccg tgggcggcgg
     2161 gtatggcgga aaggggcata tccgcatcga gccgatcgcg gcagcgctgg cgcggaaaac
     2221 gggcgggcgt cccgtgaaga tcgtcctgtc acgcgccgag gagttcgtca ctgtgacgaa
     2281 gcacgcggcg acgatcacgc tgaagacggg cgtgcggcgc gacggcacgt tcgcggcgcg
     2341 ccaggtgacg atctattgga acggcggcgc gtacgcggat gcaagtccgt cgctcgtgct
     2401 gggcggcatg gtgcgctccg tggggccgta tcggattccg gcagttcgcg tggactccta
     2461 cggcgtgtac accaacctgc cccctgcggg cgcttatcgc ggtgcgatga gttcacagac
     2521 ggcatgggcg tacgaatcgc agatggacat tatcgcgcgg aagatgggct gggatccact
     2581 ggagtttcgg tcgaagaacc tgttgcgctc cggcgagcgg ttcgcgactg gcgagacgtt
     2641 gcacgatgtg catttcgcag aatgcctcga agcggctgtg agagggctgg gacctcaccc
     2701 cccacccccc agccccctcc cccctctccc aatccaggag attgggagag gggggagggg
     2761 tggcgaagcc ggggaggggg gtgaggtcag gcgcgggcgc ggctgcgccg tgatgatgaa
     2821 gagcacggtc gccaggtcca aatcgcaggc gaagatccgc tttacggcgg acgaggtcgc
     2881 cacgctgttc accagcacgg tcgaaatggg gcagggggca cacacggcgc tggcgcaaat
     2941 caccgccgat gcgttgggtg tgccattcgg ggccgtccgc gtcgtcgggc cggacacggc
     3001 cataacgccg ttcgattcga cgacgtcggc cagccgctcg acgagcatga tgggcagcgc
     3061 ggtcagggat gcggcagagg agttgaagaa gaaactcgtc gccctcgccg cgccgatact
     3121 ggagaccccc gccgatacgt tgaaagcgga gcgtggacga atcgtcagcg ccaccgaatc
     3181 gatttcctat gccgacgtgc tcaggcgcaa tgatctcgac gcactcgaag cctccggtga
     3241 gtatgccacc gcatttggca tcgatcccga gaccgggcag ggggtatcga cgccccattg
     3301 gcatcaaggc gcaggcgctg ctgaggtcga agtggatgtc gaaacaggca gagtcacgct
     3361 ggtgcgctac agcgccgctt cgtttgcagg ccgtgtcgtc aatccgcgac tggcccagtt
     3421 gcagaacgac ggcaacgtga tctacggatt gggccccgcg cttctcgaag agatcgtatt
     3481 cgacggcgga caggtcacta atcctaatct gtccgactac atgatcccgt cgtttcgcga
     3541 tgcgccgccg gagctggtga gcacttgcct ggaatccgag ggcggtgagc tgcacggcat
     3601 cggcgagatg acgctgccgc cggtggctcc tgctatcgct aacgccgtcg aagacgcggt
     3661 cggtgtgcgg atcagagatt tgcccatcac cgccgaaaag gtgctccgtg cgctgcgggg
     3721 ggaatcggta tgaccgttct gagggtgaat ggagtcgacc gcgaagtcga tgccggcctg
     3781 cgcaccctgc tcgccgttct gcgcgacgac ctcggactgg gaggcgcaaa agaagggtgt
     3841 ggcatcggga tgtgcggtgc atgcaccgtt ctcgtcgagg gcgagccaat cagcagttgc
     3901 ctgatgctgg ccgtgcaggc ccgtgggcgc gacatcgtga ccatcgaagg gatttctcgc
     3961 gagggcgagc tgcaccgggt ccaacgtgcg ttcgtcgagt atggtgcgtt tcagtgtgcg
     4021 tactgcacgc cgggtttcgt gctctcggcg atagctcttc tgaacgagaa tccatcgccc
     4081 gacgacgatg aggtgcgtga ctatcttgcc ggcaatctgt gccgctgcgg ttcatattca
     4141 aatatcgcac gcgcagtcat cgcggccgga tggctgaagc cggcctgact gaaatcgtga
     4201 tatgcgatct tcgcttatca cgcccattgc cgtctttcta ctgtcgctcg ttggcccggc
     4261 catcgttctg ggcctgccgc tcgtgctcgg atggtcaggt gacactctct catcgcacct
     4321 gtacatcttc ggaacactcg ggcaggcgct catggacctc ctcgccatcg tcatgctcgc
     4381 tcgcagcaac ggcaaagggt tgggacgcaa gcatgagcat atagccagcc tcggcgcggg
     4441 gctggcctgc gccgccgcgc tcgccggagt caagatggct gcatcgggac gattggtgtt
     4501 tatggagggc gtgcccgcat tcacccagtc actcgctttg gcggggccat ggaatctgat
     4561 ctccgccacg gcggccgtcc tggcctatgg ccccggcgag gcgctctacg tcgtttactt
     4621 cgttaccgct ttcgacgccg cctgggagta tcaacgtcgc ctggtttcgc gaggtgtgat
     4681 catcacgacg ctgctctggg gactgtcaca tggctggaac gtgttctttc tcggctggcg
     4741 ggcgctgcct aacgcactgc ttatggttgg gattggcctg atcatcggcc tcttattcaa
     4801 gagaacccga tctccgtggg gatcaatggt cttctggtcg ttagccaatg gcacatccgc
     4861 ataggcgtgg agttgaaagc cggacggaga agatgcacgt cctggttctt gattgatttt
     4921 ctgctaatcg gttgtatact gaagctaggt gtctgtattc agcacatcgt ggacacgcag
     4981 tgtatccagc acatcgtgga cacgagagtt aggcgtattc agcacatcgt ggacatgtga
     5041 catactcgcc tccattcagg aggtgagtca tgcattccac gaagcgttcc gatcaagctc
     5101 atgcccgaca gcgccgaatt gcggtacgcc tgtatcaggc gggccacgca gtcaagagca
     5161 tcgtccagaa actcaagcgg agtcgaagtt gggtctacaa atggatcgcg tatcgcctcc
     5221 aacatccgtg gacacgcttt cgctcaggtt cgcgtgcgcc gcaccatcat cccaatcaaa
     5281 cgccacgggt cgtcacgcgg cgtgtcctgc gcctgcgaca acgattagta cggcacacgt
     5341 caccggctgt ccgatttgcc ggtgtcggag cgcggacgat ccagcgcgaa taccgcaaac
     5401 gctacgcccg cccgcccagc ctgagcgcgc tggggcgtat cctcaagcgc aaccactgcg
     5461 tcactcaggc gaagaagcgc aagcgctatc gtccccaccc ctcggccgag tatccgaatg
     5521 cagtgcaggc caccgatatc atcacgcgtt ggattcaggg tggcactgtg gtacaggcgt
     5581 tccacacgat cgatgtgtac agcaacgacg tggcctcgac gattcacgcc cacaaagccg
     5641 ccggcgacgc tcgccaacat ctgctggaaa cctggaaaac actggggatt cccgacctgg
     5701 cccagttcga caacgagagc gctttcagcg gaggccgtta tgcccgccgc atcagtcagg
     5761 tcgtgcgcct gtgcctctat ttcgggattc ccgttctgtt cacccccatc gacgaagccg
     5821 attacaactg gccggccgag acgttcaatg cgttatgggc ccagcagttc tggaaccgac
     5881 atcatttctc tcgtcggggc gatgtctccc gcgttcagcg ttcgtttctc gattggtatc
     5941 gcaccgacta tgtggcgccg cgtcagtccg acacgcccgc tcgcttgcga cgcggtcgtc
     6001 gcattcgtcg cttgccggca cgctgggccg atcaggtacc cgatcccttg cctatttatg
     6061 tcggtcagat ccaagccgtc cgccgtgtag acgccgccgg ctctgtccgc tttctcaacg
     6121 agccgattcg catcggcaaa cgctatgctg gacgatatgt ctggttgagc ctcgacaccg
     6181 gccatcagca gctcaccatc tggtatcaac cctgcgcgga agccgactgg cgtcaactca
     6241 tggtgcgcga ctatccgctg gaggaggcgg tgctgcctgt accgaagaaa tttgctcgtc
     6301 tccatatgtg agggtcgtct aacggtcgtg tccacgatgt gctgaataca tctaatggtc
     6361 ggaagttgcg ctcatggtgt ccacgatgtg ctgaatacgg atagctaggt tcaggattga
     6421 caagcgaaga cctcggagcg cacgcgatgc gccgaggcag cagcgggggc cgtgcctcga
     6481 gatcaacacc agagcttacc acatggctgt gccggtttct gcgtcgcttc tcgcgaccag
     6541 gctttacgtc ccgcgcgcag gcaagccgcc gcgcgcgccc gcgaactaca ctcagtcgga
     6601 tcccctctga agaatttctt caacggttta atcgaaccgc ttgctgcgcg cctgcgcttg
     6661 agccgcttca acctgctgcc tcgtcaattg cgcttcccat tcggcgcgca gatggccggc
     6721 gcggttcttg gtctcttcag agctggcggg gtgattcagc acaatgaaca gcaactcgag
     6781 ggcaggctcc atatcacctt ttttcgcttg caggctcgcc aagccaacaa gcgcttccac
     6841 tgccacaggc atcccacgga tttcaatcgc aatgcgcagt gactcacgcc agacgcgctc
     6901 agcttcggtg tcgtttccca gcgcgaaaac acttcgaccc atctcggcaa ggccctgcgc
     6961 cgcatagaag cgtccgccca gttcggtgaa ggtgtccacg cctttgcgga acatgtcaac
     7021 tgcccgttga tgctcgcctt gcgcctgcgc gacagcgccg agtccctggt aagcatggcc
     7081 caggttccag cgggcaccga cagatatatt cagcgcaacg ctttcttcca gcgcagtgcg
     7141 cgctacatcg tatcgtccca atgtcaatgc gatctggccg aggaaattca gcccaaaggc
     7201 cgtgaagcgc ggatcgccaa tggcgcgcca ctctgccacg gcggactgca accgctcgtg
     7261 cgcgatcttg tactcgccga tcaacatggc gatgttacct tgcagagaga gacacatcgc
     7321 tgcgaaccac tcatcgccga ctgccctggc tttctcccgt ccctcgccga ctaattcctt
     7381 tgccctggca tagttgcccg tcaaggccat gacggtgccg aggaaggtga tcggctcgac
//
LOCUS       DBNHXB010000205        10860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3234437, whole genome shotgun sequence.
ACCESSION   DBNHXB010000205 DBNHXB010000000
VERSION     DBNHXB010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10860
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3234437"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="AC162G_08280"
     CDS             complement(<1..348)
                     /locus_tag="AC162G_08280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5075848.1"
                     /translation="MNSPVSGSSSDFFDSAYCQMPPWDIGKPQPDLIALLDEFPPTGP
                     VLDVGCGTGALTFALAERGCAVLGVDLTEAAIAQARAKAVAAAPAVRQLVDFRVGDAL
                     YPAQFPGPFGAVVD"
     gene            complement(383..1360)
                     /locus_tag="AC162G_08285"
     CDS             complement(383..1360)
                     /locus_tag="AC162G_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5075849.1"
                     /translation="MMRPLVVPFLAALGLGGFAALRCQRWIRDCTRRLEGGSTVIETP
                     KGRVEYAISGAAGPAILLVHGTPGGYDQGGLLAEVAARHGFRLIAVSRPGYLRTPLEV
                     GRTPAAQADVFAALLDALGISRAAVVGLSGGGPAAIQFALRHPGRCLALVVISAVSRP
                     TVPRESFVAWLLSTRLFTSNPAGWLMGAAVKLRPALLVQALEPSPELQAEILSDPQKL
                     SALTAIAQAGIQLPAQRRAGMRNDEEQFASLPVFRVETIRVPTLVIHGTADKNVPYAH
                     GLFIAETVPGAELYAIKGGTHSIFVTHADQVFSRLFTFVGAHGTDTTVQ"
     gene            complement(1597..2292)
                     /locus_tag="AC162G_08290"
     CDS             complement(1597..2292)
                     /locus_tag="AC162G_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075850.1"
                     /translation="MKAVEEKSVLRWGGLAGILAGIFFFLTIVIAATTLLLDAGAEGP
                     VIRFPDVRAALTVVEGLYLVGLILYVTLFLALYQALRGTSLAPALGSVVGVMGLTMFA
                     VGSLVYVALAHVSGLYYAPGATSEEQATLVLIWESIQGVFSSTDSVGLLLVPIGVIVL
                     GKAMHEVPAFGKRFGRVTVVLGAVGVIGMTLLVVDPLSPTALVGIFVGAFVAIVIFPL
                     LLGWKVYSLSRAP"
     gene            complement(2631..2906)
                     /locus_tag="AC162G_08295"
     CDS             complement(2631..2906)
                     /locus_tag="AC162G_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR5075851.1"
                     /translation="MAYTITANCTACGLCLPACPIDAISVADPIYVIDQELCCDFEDC
                     LAVCPVDAIVPSDSVLSLDVQASDAERLRSRCRRGRSSIPLSAPLLE"
     gene            complement(2933..3649)
                     /locus_tag="AC162G_08300"
     CDS             complement(2933..3649)
                     /locus_tag="AC162G_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075852.1"
                     /translation="MAMFDTVTMTRQVQVFDGVARLSQNGDVREFRIKELPEKFIGWQ
                     LDYKRHIYDKIEKDEYIAFNQGHLPVVATMGSGNPFPNLANKGVGFTPKDEYLDHYLR
                     LVERAVETILALPPHAVDETRALRVATARELYAHPEHIDWRRLGLLEIFEGTTLRNLI
                     ERPLASVLWTGDSPVFISFQVDCVVEIITPEHPRYRFSWAMRRLFEYEPFHIVQTMFP
                     YSYTFWVYDWHDKTPKRRYK"
     gene            complement(3805..5028)
                     /locus_tag="AC162G_08305"
     CDS             complement(3805..5028)
                     /locus_tag="AC162G_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5075853.1"
                     /translation="MTTQTAYKPSLVSWNLTKKCNLRCPHCYLEAGPAAENELATGEC
                     LQLLDEMKSLGTEMLILTGGEPLLRKDIYDIASAASSQGFWVVMGTNGVLITDKVAQK
                     MIECGVRGVGISIDSLDPAKHNSFRGGPNAWEYAARALDICRAHGLEVLVQTTVMAMN
                     IDEIPQLIDFAREKGAWSFNLYFLVQTGRGQQMNDLSAEETEATLSNLVDAQEGYRPM
                     LVRAKCAPHFKRIAYEKGAGGLESGGCMAGTQYCRITPEGDVTPCPYMTVVAGSVRRQ
                     SFREIWETSTVLKELRDLKELKGRCGRCEFNELCGGCRCRAYAAYGDYLHEDPACTYQ
                     PTGRPLEFGAIVWSAEARARLDRIPIAFIRDKVKQGLETYARRNAARVITPDLMKEAM
                     GGAMRPEFLKSEIGD"
     gene            complement(5087..5530)
                     /locus_tag="AC162G_08310"
     CDS             complement(5087..5530)
                     /locus_tag="AC162G_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075854.1"
                     /translation="MVLVSTTDTRRRHRQAALTYAGLGLLVIAITFAAGLVPRSRTAQ
                     LAELGIGAIFIVIFSALLWRGWWPLAALLAIPNAWRAFTYFNDGQGLHIELALFSVTP
                     IEPKPVAFVNAALMAVIVVMLARSAWAGFSVWRASKKRELGKTRN"
     gene            complement(5518..6654)
                     /locus_tag="AC162G_08315"
     CDS             complement(5518..6654)
                     /locus_tag="AC162G_08315"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5075855.1"
                     /translation="MKRVLILGGGFGGIAAARRLRQKLSPEDEVMLVDRRAHFMVGFR
                     KTWALIGQSPLEAGQRPLAALAQQNIQIVQGTITAVDPASRAAEIDGRRVDADAVIVA
                     LGAELAQEKIPGFAHHAFNVYDPQDIPRASAALHEFRGGRVVVGIFGAPYKCPPAPYE
                     MALLIKDFFQARGVPVTLEVFTPQPMSLPVLGAAGCQIIEGRLAESGITFLPNHTATA
                     VEAGEIVFATGRRPYDLLLGVPPHRCPAPLAQSELTGGGNWVRVNARTMETNIPGVYA
                     VGDCVEIPLADGKPLPKAGVFAEDMGLVAAERIAATFAGQAADATFKGEGGCFLEVGD
                     GQALMVQGAFLTEPAPQVSLSEPSRQYLEEKRAFEETRLQAWFS"
     gene            complement(6753..7112)
                     /locus_tag="AC162G_08320"
     CDS             complement(6753..7112)
                     /locus_tag="AC162G_08320"
                     /inference="COORDINATES: protein motif:HMM:NF019968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075856.1"
                     /translation="MSVTFRCPTCGRAIEMVTNPGETQMVRSLGVTIGHEALNAQPEP
                     MAMIRDALAGGSVAATTGPDPIWTDAALKRLAAAPTFVQGMVRRLYTDYARQKGYTEI
                     TPAVMTEAREALGMTGM"
     gene            complement(7263..8129)
                     /locus_tag="AC162G_08325"
     CDS             complement(7263..8129)
                     /locus_tag="AC162G_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR5075857.1"
                     /translation="MTNHSAVHDKSNVLGQVHAQRERVAARLAHIQRVVAVMSGKGGV
                     GKSALTANLAAALAMQGRAVGVLDADIHGASAAAMLGARGQRVVIGENGARPAIGVAD
                     IRVMSMDLFLSDEDDPVTWRHPGGLAADTFVWRGTLEANALREFLSDTDWGELDFLLI
                     DMPPGADRFETLMRLVPNLSGALVVTIPSQVSHLVVRRAIAAARQADARLLGLVENMA
                     GLAEGEVTHELFPNGRGEDFAAEVDVPYLGRVPFDPQLARTTDSGRPFVIEHAESRAG
                     RAILEIAAVLER"
     gene            8209..8529
                     /locus_tag="AC162G_08330"
     CDS             8209..8529
                     /locus_tag="AC162G_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017745980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR5075858.1"
                     /translation="MQLEDYFDFLAPDDIRIKDHRVGIESVLYEYIHRDQTPEAIARR
                     FPTLTLEQIYATILYYLHNREKIDAYLTDWLEYGRRMREEQDRNPPPVVVHLRALKAE
                     RQEA"
     gene            8537..8902
                     /locus_tag="AC162G_08335"
     CDS             8537..8902
                     /locus_tag="AC162G_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002732058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HIR5075859.1"
                     /translation="MSNIRYLLDENVDPLFRRELLKREPTLVVWNIGSPGAPPARTRD
                     PDILLWCEANTFILVTNNRKSMPRHLRDHLAEGRHIPGIFELNPDMGIGATIEELLLI
                     WAASLEDEYRDRIVYLPLS"
     gene            complement(8884..9204)
                     /locus_tag="AC162G_08340"
     CDS             complement(8884..9204)
                     /locus_tag="AC162G_08340"
                     /inference="COORDINATES: protein motif:HMM:NF022077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR5075860.1"
                     /translation="METRRAGDKLNVLGQVLAQRERVAVRLSHIRRVVAVMSGKGGVG
                     KSALAANLAAALAMQGRAVGVLDADIHGASAAMIGARKRNRRWRAAVQDYSRVCCQRQ
                     LRGR"
     gene            complement(9282..9512)
                     /locus_tag="AC162G_08345"
     CDS             complement(9282..9512)
                     /locus_tag="AC162G_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075861.1"
                     /translation="MASLSFAQDIRPLFRETDVEEMIDVAGFDLSRYEDVRDRALDIY
                     ERLGDGSMPCDGAWPADQVARFKQWMDDGMAP"
     gene            complement(9536..>10860)
                     /locus_tag="AC162G_08350"
     CDS             complement(9536..>10860)
                     /locus_tag="AC162G_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5075862.1"
                     /translation="RQRYSQLGGVVARTLRGTLKDTLIVRDDKPLSGGRIMVCIDGSA
                     YCYKGMRAALELAEAFGGSLYVCSAFDVEYHHVVFHNIKDVLSYQASKVFKFEEQEEL
                     HNNIIDRGLRKVCEANLMRAEKVAQEECLPITTEVLVGKPFNKILEWCEKENPDLVVI
                     GRWGAHHVEGADMGSNTENLLRQCACNLLIVGAADAKPYDIPTLEEYEEEPLEWDDDA
                     LKLILRAPPFAQSMAKASVEEYARKEGYTRITLDLLHGALMGILPPSAKHLMGLEAED
                     RKIAEARKSARSPIEEYVPHERAFPWTQEALDRVSSRVPAFVRPIAMLAMERYAREKQ
                     LPEVTLDVTIEVAKRLGYAGDDGVQPSMPWTDEALVRLERVPKFERQMVRQMVESLAH
                     DAGHSAINYEVADQMLAKVREMWEAQSEGAFGYFKGMAGNGEEGKSGN"
BASE COUNT         2117 a         3515 c         3098 g         2130 t
ORIGIN      
        1 gtcgaccacc gccccgaacg gtcccgggaa ttgggcggga tagagtgcgt cgccgacgcg
       61 gaagtctacc aactgtcgga cagccggcgc agcggcaacc gccttggcgc gggcttgcgc
      121 gattgcggct tcggtaaggt ccacacccaa aacggcgcag ccacgctcgg ctagggcaaa
      181 cgtgagtgcg ccggtgccac agccaacgtc aagcacgggg ccagtaggtg gaaactcatc
      241 cagcagcgcg atcagatctg gctgcggctt gccgatgtcc cagggtggca tctggcagta
      301 agccgaatcg aaaaagtcgc ttgatgaacc ggaaacagga ctgttcatga atacttcccc
      361 atcgcgttgt tatgcgccac tctcattgta cggtcgtatc cgtgccgtgt gcaccgacga
      421 acgtgaataa ccgcgagaac acctggtcgg cgtgtgtcac gaagatactg tgcgttccac
      481 ccttgatagc gtacagctcg gcaccgggaa ctgtttcggc gatgaatagg ccgtgggcgt
      541 atggaacgtt cttgtcggcc gtcccgtgta tgaccagcgt tgggacacgt attgtctcga
      601 cccgaaacac gggcagactg gcgaactgtt cttcgtcatt ccgcattcca gccctacgtt
      661 gcgcggggag ctgtattcct gcctgggcaa ttgcagtgag cgcggaaagc ttctgcggat
      721 ccgacagaat ctctgcctgg agttcgggac tgggctcgag tgcctgaacc aagagagccg
      781 gccgcagttt cacggcggct cccattagcc aaccagccgg attggatgtg aaaagccttg
      841 ttgacagtaa ccaagccacg aagctctcgc gagggactgt cgggcgactc accgctgaga
      901 tcaccacgag ggccaggcac cttccagggt gccggagagc aaactggata gcagccggcc
      961 cgccacccga taggccgacc acggcagcgc gggagatgcc gagcgcatca agcagtgcgg
     1021 cgaagacatc ggcctgtgcc gccggtgtcc gcccgacctc aagaggcgtt cgcagatacc
     1081 ccggacgtga caccgcgatc agccggaagc cgtgccgtgc agcgacctca gccaacaacc
     1141 ctccctggtc gtatccacct ggcgtaccgt gcacaagcag gatggccgga ccggcggctc
     1201 ctgatatggc gtactcgacc cggcccttcg gcgtctctat taccgtactg ccgccctcga
     1261 gcctccgagt gcaatcgcgg atccatctct gacagcggag cgccgcaaat ccgccgagtc
     1321 cgagtgcggc taagaagggg acaacaagag gcctcatcat ctcggcctcc aaatcctgct
     1381 tatttggtgg tttcgaagcc aggcgacgtt ctctccaagc atcgacacca agtaccgcct
     1441 tttgtgtcct ttataggcat ccctgtgagc ctaggcgatt tgttccaacg gatagtgtct
     1501 atctatggcc gattttgtct tccccgcctc cattatctct ttgaggaagc ctagatcttc
     1561 agttcattgg ggtgctatcc aatatcttca agacccctac ggggcccttg acaggctgta
     1621 gaccttccat cccaggagga gagggaagat gacgatggcg acaaacgcgc cgacgaagat
     1681 gccgaccagg gctgttggag agagaggatc aaccacaagg agcgtcatcc ctatgacccc
     1741 aaccgcgccg agcaccacag tcaccctgcc aaagcgcttg ccaaaggccg ggacctcgtg
     1801 catagccttt ccgaggacga ttacacctat cggcacgagc aggagcccga cagagtccgt
     1861 cgaactgaat acgccctgaa tcgactccca tattaggacg agcgtcgcct gctcctcgga
     1921 ggtcgcccca ggagcatagt agaggccgga gacgtgggcg agagcgacat acactagaga
     1981 gccgacagcg aacatggtga gacccatcac tcccacgacg ctcccaagag cgggcgcgag
     2041 gctcgtcccc cggagagcct gataaagggc aaggaagagc gtgacataca agatgagtcc
     2101 taccaggtag aggccttcca ccacagtaag ggccgctctg acgtcgggga accttatgac
     2161 cggcccctcg gcgccggcat ccagtagcag ggtcgtggct gctatgacga tagtgagaaa
     2221 gaagaagatg ccagccagga tacccgccag accaccccat cgcagcacgc tcttctcctc
     2281 cactgctttc atgagtctcc tccaaggctg ctttgatccg gtcattttcg atacactaca
     2341 ctgtgatcca tcccatcagc ataggttttg aaaacctaac tcgggattat ccaacaaaca
     2401 tgtcgtacat tatctccgag agggcaatta tccggcgatt ggatgattgc atatatccga
     2461 cttgaaggtg gttggattgt gtccgtgtat cctcagtata gatcggtttc ttcgcaagtc
     2521 aatacgcggc ggtcgtctcc cgtggctttt acattttcgt ctggcgtcgt gactgatgcg
     2581 tgggcgcggt agtagtaact gctgcgcggg tagtccagca cgtggtcgaa tcactcaaga
     2641 aggggggcgc tcaagggaat gctggaacgc ccgcgcctgc agcgcgagcg caggcgttcg
     2701 gcgtcagacg cttgaacatc caacgacaga acgctatccg agggaacgat agcatcgacc
     2761 ggacacacgg ccaggcagtc ctcaaagtcg cagcagagct cctggtcgat cacgtagatc
     2821 ggatcggcga ccgagatggc atcgatcggg catgccggca agcacaggcc gcaggcggtg
     2881 cagttggcag tgatagtgta ggccatggtg gcttagcgat aagcgataag catcatttgt
     2941 accgccgttt cggcgtcttg tcgtgccagt cgtagaccca gaaggtgtac gaatatggga
     3001 acatggtctg gacgatgtgg aaaggctcgt actcaaacag ccgccgcatg gcccacgaga
     3061 agcgatagcg cggatgctcg ggcgtgatga tctccacgac gcaatcgacc tgaaaactga
     3121 tgaacaccgg cgagtcgccc gtccacagca cggaggccag cggccgttcg atcaggttgc
     3181 ggagcgtcgt gccctcgaat atttcgagca atcccagtcg ccgccagtcg atgtgctcgg
     3241 gatgcgcgta cagctcgcgg gccgtggcga cgcgcagcgc gcgcgtctcg tcgacggcgt
     3301 gcggcggcag ggccaggatc gtctcgaccg cccgttcgac gagccgcagg tagtgatcca
     3361 gatactcgtc tttcggcgtg aagccgacgc ctttattcgc caagttcggg aagggattgc
     3421 cgctgcccat cgtcgcgacc accggcaaat ggccctgatt aaaggcgatg tactcgtcct
     3481 tctcgatctt gtcgtaaatg tgcctcttgt agtccagctg ccagccgatg aacttctccg
     3541 gaagctcttt gatccgaaat tcgcggacgt ccccgttctg gctcagccga gccacgccat
     3601 cgaacacctg cacctgccgt gtcattgtca ccgtatcgaa cattgccatc gagcctctcc
     3661 ttgccgtaaa gtgattggag attggcaatt agaggttagc cgatctccaa tctccaacct
     3721 ccaatctcca acctccaacc tccgatctcc aacctccaac ctccaatctc caacctccaa
     3781 tctccaatct ccaacctcta atctctaatc tccaatctcc gatttcaaga actccggtcg
     3841 catcgcgccg cccatagcct ccttcatcaa gtccggcgtg atcacgcgcg ccgcgttgcg
     3901 tcgagcatag gtctccaggc cctgtttgac tttgtcgcgg atgaaagcga tagggatacg
     3961 atcgagccgg gcgcgggctt ctgcgctcca gacgatcgct ccgaactcca gcggccggcc
     4021 ggtcggctgg taggtgcagg ctggatcttc gtgcaagtaa tctccatacg cggcgtacgc
     4081 ccggcagcgg caaccgccgc acagctcgtt gaattcgcag cgcccgcagc gccccttcag
     4141 ttccttcaga tccctgagtt ccttcagcac cgtcgatgtt tcccagatct cgcgaaaact
     4201 ctgccttcgc acgctgcctg cgacgacggt catatacggg cacggtgtga catcgccctc
     4261 tggcgtgatg cggcaatatt gggtgccggc catgcagccg ccgctttcca gcccacctgc
     4321 gcctttttcg taggcgatgc gcttgaaatg gggcgcgcat ttcgcgcgca ccagcatcgg
     4381 gcgatagccc tcctgcgcat cgaccagatt cgaaagggtg gcctccgtct cttccgccga
     4441 cagatcgttc atctgctggc cccggccggt ctggacgagg aagtacagat tgaacgacca
     4501 ggcgcccttt tcgcgcgcga aatcgatcag ttgcgggatt tcgtcgatgt tcatggccat
     4561 gacggtcgtc tgcaccagca cttccagacc atgcgcgcgg caaatgtcta acgcccgagc
     4621 cgcatattcc caggcgttcg gccctccccg gaagctgttg tgcttggccg gatcgagcga
     4681 atcgatgctg atgcccacac cccgcacgcc gcattcgatc atcttctgag cgactttgtc
     4741 tgtgatcagg acgccgttgg tgcccatcac gacccagaaa ccctgactcg acgcagcgct
     4801 cgcgatgtcg tagatgtctt tgcgcaagag cggctcgcct ccggtcaaga tgagcatctc
     4861 cgtgccgagc gatttcatct cgtcgaggag ttgcaggcac tcgcccgtcg ccagctcgtt
     4921 ctcggccgcc gggccggcct ccaggtagca atgcgggcag cgcaaattgc acttcttcgt
     4981 caggttccag gagacgagcg aaggcttgta cgcagtctga gttgtcatca ttttgatggc
     5041 tcttgatgaa agggaactca gggaactgag ggaacttgca gttccctcag ttccttgttt
     5101 tccctagttc cctctttttt gacgcgcgcc ataccgaaaa gcctgcccac gcggagcgcg
     5161 ccagcatgac gacgatcacg gccatcagcg ccgcgttgac gaaggcgacc ggcttcggct
     5221 cgatcggcgt cacactgaag agcgccagct cgatgtgcag gccctgtcca tcattgaaat
     5281 aggtgaacgc tcgccaggcg ttcgggatcg cgagcagagc cgcaagcggc caccaaccgc
     5341 gccagagcag cgccgaaaag atcacgatga aaatggcgcc gatgcccagt tcggcaagct
     5401 gcgctgtccg gctgcgcggc accaaaccgg cggcgaaggt aatcgcgatg accagtaggc
     5461 cgaggccggc gtacgtgagc gcggcctggc gatggcgccg ccgcgtatcc gtcgtgctca
     5521 cgagaaccat gcttgcagcc gcgtctcctc aaacgcgcgc ttctcttcga gatactggcg
     5581 cgatggctca gaaagcgaca cctggggagc cggctcggtc agaaatgcgc cctgcaccat
     5641 cagcgcctgg ccgtccccca cctccaggaa gcacccgcct tctcccttga acgtggcgtc
     5701 tgctgcttgc ccggcgaacg tggcggcgat ccgctctgcg gcgaccaggc ccatgtcctc
     5761 ggcgaaaacg ccagccttcg gcagcggctt gccgtccgcc agggggattt cgacgcaatc
     5821 gccgacggca tacacgccgg ggatgttagt ctccatcgtg cgcgcgttca ctcgcaccca
     5881 gttgccaccg ccggttaact cgctctgcgc cagcggcgcc gggcagcgat gcggtggcac
     5941 gccgagcagg agatcgtaag gacgccggcc ggtcgcaaac acgatctcgc ccgcctcgac
     6001 ggccgtcgcc gtgtggttgg gcagaaaagt aatgccactt tcggccaggc gaccttcgat
     6061 gatctggcag cccgcggcgc ccaggaccgg cagcgacatc ggctgcgggg tgaaaacctc
     6121 cagggtgacc ggcactccgc gcgcctggaa gaaatccttg attagcaaag ccatctcgta
     6181 cggggccggt gggcacttgt agggcgcgcc gaagatgcca accaccaccc gccctccgcg
     6241 aaattcatgc agggccgcgc ttgcacgagg aatatcctgc ggatcgtaaa cgttgaaagc
     6301 gtgatgtgca aagccgggta ttttctcctg cgccagctcg gcgcccaacg ccacgatcac
     6361 ggcgtcggca tcgacccgcc ggccgtcgat ctccgccgcg cgagacgccg gatccacggc
     6421 ggtaatcgtg ccctggacaa tctgtatatt ctgctgggcc agcgccgcca gtggacgctg
     6481 cccggcttcc aacggagact ggccgatcag cgcccatgtc tttcggaagc cgaccatgaa
     6541 atgcgcccgc cgatcgacga gcatcacctc gtcttccggc gagagctttt gccgcagacg
     6601 gcgtgctgcc gctattccgc caaagccgcc acccaggatc aacacacgtt tcatcgatac
     6661 tcctttggcc gatggctatt agctgatggc tgatagccga tggttttaca ccatctgcca
     6721 tctgctatct gctatccgct atctgccatc tgctacatcc ctgtcatccc taacgcctcg
     6781 cgggcttcgg tcatgaccgc cggtgtgatc tcggtgtagc ccttttgtcg ggcgtaatcg
     6841 gtgtacaggc ggcggaccat gccctgcaca aaggtcggcg cggcggccag gcgcttgagc
     6901 gccgcatccg tccatatcgg atcgggaccc gttgtggccg ctaccgatcc gcccgccagt
     6961 gcgtcgcgga tcatcgccat cggctcgggc tgagcattga gcgcctcgtg cccgatcgtc
     7021 acgcccaggc tgcgcaccat ctgcgtctcg ccaggattcg taaccatctc gatcgcgcgt
     7081 ccgcaggtcg ggcatctgaa agtgacagac atcgacccgc cgctctccga ggcaatgctc
     7141 tccacaaatt ccattgcagt gttatcggac tgacagacaa atttcatggc aggaggaact
     7201 cctgaaattg aggagactga gttcctttag ttccttcagt tcctctagtt ccctcgtttc
     7261 cttcagcgct caagaaccgc cgcgatctcc aggatcgctc gccctgccct gctctcggcg
     7321 tgttcgatga cgaagggccg gccggagtcg gtcgtccgcg ccaactgcgg gtcaaacggc
     7381 acacgcccca agtaaggcac gtccacctct gcggcgaagt cctctcccct cccattcggg
     7441 aacaactcgt gtgtgacctc tccctccgcc agtccggcca tgttttccac cagacccaac
     7501 aagcgggcgt ccgcctgtcg cgccgccgca atcgcacgcc gcacgaccag atgcgaaacc
     7561 tgcgagggga tcgtgacgac gagcgcgccg gagagattcg ggaccagtcg catcagcgtc
     7621 tcgaagcgat ccgcgccggg cggcatatcg attaacagga agtctaattc tccccaatcc
     7681 gtatcggaca ggaactcgcg cagtgcgttg gcctccagcg tgccacgcca cacgaatgta
     7741 tcggccgcca gcccgcccgg atgacgccac gtgaccgggt catcctcatc actcaggaag
     7801 agatccatcg acatgacgcg tatatcggcg acgccgatcg ccggtcgcgc accgttctcg
     7861 ccaatcacca ctcgctgtcc gcgcgcgccg agcatcgccg ctgccgaagc cccgtggatg
     7921 tcggcgtcga gcaccccgac cgccctaccc tgcatcgcca acgccgccgc cagattcgcc
     7981 gtcagcgccg acttgccgac gccgcccttg ccgctcatca ctgcgacgac gcgctgaatg
     8041 tgcgccagtc gcgcagcgac gcgctcgcgt tgggcatgca cctggccgag gacgttagat
     8101 ttgtcgtgta cggcgctatg attagtcacg gatgaatttg acccataagt ttccatgctt
     8161 tgtagtataa ttcacgttaa agccatttcg acgcttgggg tgaaagccat gcaacttgaa
     8221 gactatttcg acttcctcgc acctgatgac attcgaataa aagatcatcg agtcggcatt
     8281 gagtcggtgc tttacgagta cattcaccgt gatcagactc ctgaagccat cgctcggcgc
     8341 ttccccacgt tgactctcga gcagatttac gccacgattc tctactactt gcacaaccgc
     8401 gagaaaattg acgcttatct gacggattgg ctggaatatg gtcgccggat gcgggaagag
     8461 caagaccgca atcctccgcc ggtagtggta cacttgcgtg ctttgaaagc agaacgtcaa
     8521 gaagcatgac cactccatga gcaacattcg ttatttgctg gacgagaatg ttgacccgct
     8581 gttccgccgc gaattactga aacgtgagcc gacgttggta gtttggaaca ttggctcgcc
     8641 gggcgcgccg cctgcgagga cacgcgatcc ggatattctg ctttggtgcg aagccaacac
     8701 cttcatcctg gtcacaaaca accgcaagtc catgcctcgg catttgcgcg atcatctggc
     8761 ggaaggtcgg catattccag gtatctttga actgaacccg gacatgggca tcggcgcaac
     8821 cattgaagaa ttgttgctga tttgggcagc atcacttgaa gacgaatacc gcgatagaat
     8881 cgtttacctg cctctgagtt gacgctggca gcagacacgt gagtaatctt gaaccgccgc
     8941 tctccagcgg cgatttcttt tgcgcgcgcc gatcatcgcc gctgaagcgc cgtgaatgtc
     9001 ggcgtcgagc acgccgaccg ccctaccctg catcgccagc gccgccgcca ggtttgccgc
     9061 cagcgccgac ttgccgacgc cgcccttgcc gctcatgacc gcgacgactc gcctgatgtg
     9121 tgacaggcgc acagccacgc gctcgcgttg ggcgaggact tggccgagca cattcaattt
     9181 gtcgccggcg cgacgtgttt ccacatgagt cgctcaacaa agtgtacggg caggtcttag
     9241 ccctgccctc caagaatgcc tggcacgcga gcgccgcttc ctcacggcgc catgccgtcg
     9301 tccatccatt gcttgaacct ggcgacctga tcggccggcc aggccccatc gcagggcatc
     9361 gaaccgtcgc ccaggcgctc gtagatgtcc agtgcgcgat cgcgaacatc ctcgtacctg
     9421 gagagatcga agccggccac atcgatcatc tcctcgacgt cggtctcgcg gaacaacggc
     9481 cggatgtcct gtgcaaaact gagtgatgcc atgagcactc ctcctgtgaa ttccttcagt
     9541 tccctgactt cccttcttca ccgttccccg ccatcccctt gaaatacccg aacgcgcctt
     9601 cgctctgcgc ctcccacatc tcacgcactt tggcgagcat ttgatcggcc acttcgtaat
     9661 tgatcgccga atgtccggca tcgtgcgcca gactctcgac catctgacgc accatctgcc
     9721 gttcgaactt gggcacgcgc tccaaccgaa ccagcgcctc atccgtccac ggcatcgacg
     9781 gctgcacgcc atcgtcgcca gcgtacccca accgcttggc gacttcgatc gtcacgtcca
     9841 gcgtcacttc gggcaactgt ttctcgcgcg cgtagcgctc catcgccagc atcgcaatcg
     9901 gacggacgaa ggccggcacg cggctgctca cgcggtcgag cgcctcctgc gtccagggga
     9961 aggcgcgctc gtgcggcaca tactcttcga tcggggagcg ggcggacttg cgggcctccg
    10021 cgatcttgcg gtcttcggcc tccagcccca tcagatgctt ggccgagggc ggcaggatgc
    10081 ccattaacgc gccgtgcagc aggtcgagcg tgatacgcgt gtagccctct ttgcgcgcgt
    10141 attcctcgac cgatgccttc gccatcgact gcgcgaacgg cggtgcgcgc agaatgagct
    10201 tcagcgcgtc gtcgtcccat tcgagcggtt cttcctcgta ttcttcgagt gtcgggatgt
    10261 cgtacggctt cgcgtcagcc gcgccgacga tgagcaggtt gcacgcgcat tggcgcagca
    10321 gattctcggt gttcgatccc atgtcggcgc cctcgacgtg atgcgccccc cagcgtccga
    10381 tcacgaccag atcgggattc tccttctcgc accactccag gatcttattg aacggcttgc
    10441 cgacgagcac ctcggtcgtg atcggcaggc actcttcttg agccactttc tcggcgcgca
    10501 tcaggttggc ctcgcacacc ttgcgcaggc cacgatcgat gatgttgttg tgcagctcct
    10561 cctgctcctc gaacttgaaa accttcgaag cctggtagga cagcacgtcc ttgatgttgt
    10621 ggaacacgac gtgatggtat tccacgtcga acgcactgca cacgtagagc gatccgccga
    10681 acgcctcggc cagctcgagc gccgcgcgca tgcccttgta gcagtacgcg gagccgtcga
    10741 tgcagaccat gatgcggccg cccgagaggg gcttgtcgtc gcggacgatc agcgtgtcct
    10801 tcagggtccc gcgcagcgtg cgcgccacga cgccgccgag ctgcgagtac ctctggcggc
//
LOCUS       DBNHXB010000206         2820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_718353, whole genome shotgun sequence.
ACCESSION   DBNHXB010000206 DBNHXB010000000
VERSION     DBNHXB010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_718353"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="AC162G_08355"
     CDS             <1..202
                     /locus_tag="AC162G_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR5075863.1"
                     /translation="FDTRIGTFFDKPMLETTCVFCGQCVGVCPTGALKSKREWLLEEG
                     RTPDEIMNLTRAERRKSRKERK"
     gene            412..669
                     /locus_tag="AC162G_08360"
     CDS             412..669
                     /locus_tag="AC162G_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075864.1"
                     /translation="MVTRAIVQEKILAYLNRPITLTELVDWAEEAMMDGEFDERDIDL
                     LTEIVSRLGLADVREFGLSWDDCYEFLGRLGYRIEVTAVPA"
     gene            733..1539
                     /locus_tag="AC162G_08365"
     CDS             733..1539
                     /locus_tag="AC162G_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR5075865.1"
                     /translation="MASAACWGTGDFSGGLATKRTNVYAVIIASQIVGAVLLAMLALA
                     FREPLPLPDQSLWGMLAGLAGAVGLIGLYRALALGRMGVAAPVSAVITATVPVVVGAF
                     TEGMPDVPQQIGFGLALIGIWLVSRTEGLRVRIGDLGLPIMAGLSFGFFLVFIGQVSD
                     GAVLWPLVAARTASLTLMIVTATLTRQPRLPEMKALPLIALAGAMDAGGNAFFALAAQ
                     AGRLDVASVLASLYPATTVWLAWLILKERIARPQAFGIVVTLAAITLIVA"
     gene            1791..>2820
                     /locus_tag="AC162G_08370"
     CDS             1791..>2820
                     /locus_tag="AC162G_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5075866.1"
                     /translation="MIQADRVVPTTCPYCGVGCNLELHIKNDTIYRVTSPFDSVVNHG
                     NLCVKGRFGYDFIYSPARVTTPLIRRTPQSPGHRTQAFDPAEWREVSWDEALHYTADR
                     LVEIYRRDGSDAMAVYCCAKATNEDNYLLQKMFRALFRTNNVDHCTRLCHAGSVVALL
                     KAIGSSAMSNTAAEVIKSDVFIVTGSNTTENHPIIALQMKDAVMKHGAKMIVIDPRRI
                     ELCDFATLWLPVKPGTNVPVFTAMAHVIIREGLVNKEFVEERTEGYEEFMQSVEKFTP
                     EYAEAISGVDRNLIVEAARMYATAKNGAIYWGMGISQLSHGTASALALIHLAFLTGHI
                     GRDGTGLNP"
BASE COUNT          549 a          825 c          892 g          554 t
ORIGIN      
        1 cttcgatacg cgcatcggca ccttcttcga caagccgatg ctggagacga cgtgcgtgtt
       61 ctgcgggcag tgcgtcggcg tgtgccccac cggcgcgctc aaatccaagc gtgagtggct
      121 gctggaggag ggcaggacgc cggatgagat tatgaacttg acgcgggcgg agaggaggaa
      181 gagcaggaaa gaaaggaaat aaaggaaatc agggaacttc ccgtgtgtac ccgcgcgcag
      241 agcgagcgta ggatcaagcg gagtaactac tcagccgtcg tcattgcgag cgattcttgc
      301 gaagcaatct ccgcgccaat cagcaagcag ggccatctat gaaggggatt gcctcgtcgc
      361 aaaagacgct cctcgcaatg acggccgagc agttatcaag cggactgaaa tatggtcaca
      421 cgcgccatcg ttcaagagaa aatcctggcc tatctgaatc gccccatcac gctgactgag
      481 ttggtcgatt gggccgaaga ggcgatgatg gatggcgagt ttgatgaacg agatattgat
      541 ttgctgacag agatcgtctc gcggcttggc cttgccgacg ttcgcgagtt tggactgtct
      601 tgggacgact gctacgagtt tctggggcgg ctcggatatc gtatcgaagt aacggctgtt
      661 ccggcctgaa gtgggtcgca ccgaaaggat gagagtctga tcggatcaga acctgcgact
      721 gtcgccttcg ggctggcctc ggcggcgtgc tggggcacag gcgatttcag cggcggcctg
      781 gcgacgaagc ggacgaacgt ctacgcggtc atcatcgcgt cgcagatcgt cggcgctgtc
      841 ttgctcgcca tgttggcgct cgctttcagg gaaccgttgc cactccccga tcaatcactg
      901 tggggcatgc tggccgggct ggcgggcgcg gtcggtttga tcggcctgta ccgcgcgctg
      961 gcgcttggac ggatgggcgt ggcggctccc gtctcggcgg tgatcacggc gaccgtgccc
     1021 gtcgtcgtcg gcgcgtttac cgagggcatg ccggacgtac cgcaacaaat cggtttcgga
     1081 ctggcgctga ttggcatctg gcttgtatcg cgcacggagg ggctgcgagt tcgtatcggc
     1141 gaccttgggc tgcccatcat ggccggactg agctttgggt tctttctggt cttcatcggc
     1201 caggtgagcg atggggcggt gctgtggccg ctggtcgcgg cgcggacggc atcgctgaca
     1261 ctcatgatcg tgacggcgac gctcacccgc cagccacggc tgccggagat gaaagcgctg
     1321 ccactcatcg cactggctgg cgcgatggat gccggcggga acgccttctt cgcgctcgcg
     1381 gcacaggcgg gccggctgga cgtggcgtct gtgttggcat cgctctaccc ggcgacgacc
     1441 gtctggctcg cgtggctgat tttgaaggag cgcatcgcac gtccacaagc gttcggcatt
     1501 gtcgtgacgc ttgcagctat caccctgatt gtggcataag cgtacccgcc acaacccgat
     1561 actcgggcgc gaattttctg gataaaagta actactcagc ccgcgtcatt gcgagcgttt
     1621 tttgcgaagc aatctcagtg cctatccgca cacatcgcca gtcgtgtagg agactgcttc
     1681 gtcgcacaca acgctcctcg cagtgacggc tgagcagtta cggataaaat tacaatatca
     1741 ttgtcgatag cccgaagcga catctccggg cggctctgaa ggaggttgcc atgattcagg
     1801 ctgacagggt tgttcccacc acctgcccct actgcggcgt gggctgcaat ctcgagctgc
     1861 atatcaagaa tgacaccatc tatagggtca cctcgccctt cgacagcgtc gtgaaccatg
     1921 gcaacctgtg cgtcaaaggg cgctttggct acgactttat ctacagcccg gcgcgcgtga
     1981 cgacgccgct tattcgccga acgccgcaat cgcccggcca tcgtacccag gcgttcgacc
     2041 cggcagagtg gcgcgaggtc tcgtgggacg aggcgctcca ctacaccgcc gaccggctgg
     2101 tcgaaatcta caggcgcgac ggctcggacg cgatggcggt gtactgctgc gccaaggcga
     2161 cgaacgagga caactatctc cttcagaaga tgtttcgcgc cctgttccgc accaacaacg
     2221 tcgaccactg cacgcggctg tgccacgccg ggtcggtggt ggcgctgctg aaggcgatcg
     2281 gttcctcggc gatgagcaac acggccgccg aagtgatcaa gagtgacgtc ttcatcgtca
     2341 ccggctcgaa caccacggag aaccatccca ttattgcgct gcaaatgaaa gacgcggtga
     2401 tgaaacacgg cgcaaaaatg atcgtgatcg acccgcgccg catcgagctc tgcgacttcg
     2461 ccacgttgtg gctgccggtc aagccaggga cgaacgtgcc ggtcttcacc gcgatggcgc
     2521 atgtcatcat cagggagggg ctggtcaata aggagttcgt cgaagagcgc accgaaggct
     2581 acgaagagtt catgcaatcc gtcgagaagt tcacgcccga atacgccgag gccatctcgg
     2641 gtgtcgatcg caatctcatc gtcgaagcag cgcgcatgta cgccacggcc aagaacggcg
     2701 cgatctactg gggcatgggc atctcccagc tttcccacgg aaccgcttcg gcgctggcgc
     2761 tcatccacct ggcatttctt accgggcaca tcgggcgcga tggtaccggc ttgaatccct
//
LOCUS       DBNHXB010000207        25061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2395875, whole genome shotgun sequence.
ACCESSION   DBNHXB010000207 DBNHXB010000000
VERSION     DBNHXB010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 25061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 25061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25061
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2395875"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="AC162G_08375"
     CDS             <1..187
                     /locus_tag="AC162G_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIR5075867.1"
                     /translation="EQAIVATAHKIARAYYHVLKHRTPFRSLSADEDEQQVRQREVAH
                     LKKKAAKLGFTIVETPA"
     gene            complement(310..1527)
                     /locus_tag="AC162G_08380"
     CDS             complement(310..1527)
                     /locus_tag="AC162G_08380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5075868.1"
                     /translation="MAEKKSKKNRARRKPARARAEPAAETALAIPEPEVEYTRPEAEP
                     EVETVEVSLRQQLIHELDELAKRLRAITPGYSAAPATTGSVIDVIEEGADRIAPGFAR
                     RLRDTLSRFINEDYLDVDTWKGMWTVLNQTVQYQTDVFRRRLTGEYETDEWGLDREFM
                     DAVRPFFEFMYKKYWRVQMTGVENIPAEGRALLVVNHSGQLPWDGAMLGIGVMLEHPN
                     QRLVRNLYATWFPTLPFFSDILVKMGQVMATEDNGIRLLEQDELAAVFPEGYKGVGKL
                     FKERYRLARFGRGGFIKMALKTCAPMIPVAIVGAEETYISLAKSNVMAKITGFPYFPI
                     SVTWPWLGPIGFIPFPTKWTIDIGEPIPMDHYGRDADQSLVLVSQLTDQVRNIVQDMI
                     YARLAKRRSVLFG"
     gene            complement(1564..2685)
                     /locus_tag="AC162G_08385"
     CDS             complement(1564..2685)
                     /locus_tag="AC162G_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5075869.1"
                     /translation="MTEKRAILVTGVNAFWGSRVAAQLAARADLHVIGLVDTPPEEVI
                     EGLDFIQADVRNPLVVKLLEAERVDTVCHVTFVETARSSELAFDLNVMGTMKLLGACA
                     EAGVRKVVLKSSTMVYGANPGNSAFLPEDYPLQASQSYEYTRDLVEIEAFCNGFRGQA
                     PGVLLTILRFAHIVGPKADTPLTRFLREEQAPSLLGFDPMMQVIHEDDVVRALLHVIL
                     NDVPGVFNVAAEGVMPLWKVMGLAGKLPIPFLHPLAYLGVSLLGPAFAPIALDYLRYP
                     CVGDLGKMRTELGFVPQYTAEEALREFAAQQRLRRYLPEAAARAYDEERLHDTIERRR
                     RARTLDSEPSAPRSRARASSKRTPPARCRTQRRAKTVKR"
     gene            complement(2718..3149)
                     /locus_tag="AC162G_08390"
     CDS             complement(2718..3149)
                     /locus_tag="AC162G_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075870.1"
                     /translation="MSTFRDSAQFYDCVGELMDRAKKDPKVGPKIAKSGIIIQFRYTD
                     PEAMTTINARDKPTQADAFVDVIHGACNLRPDVTMSMKADVAHAFWHGKVNLVAALAK
                     KEIVASGPVPKILKLVPAVEPLYKVYPALLREKGYGNLVLK"
     gene            3481..3855
                     /locus_tag="AC162G_08395"
     CDS             3481..3855
                     /locus_tag="AC162G_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HIR5075871.1"
                     /translation="MTLKQKKAVETEALAPENGHESLLEPLHKAMLAGIGAMAMGVDA
                     VDKVTGRFIERGELVEKDAQKLWRQFAKEFQKRQKRAERGVGKELEDMREAMDIPSKA
                     DIDALGEKIAALSTKVDALKAS"
     gene            3940..4515
                     /locus_tag="AC162G_08400"
     CDS             3940..4515
                     /locus_tag="AC162G_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HIR5075872.1"
                     /translation="MLVIKRYSNRKLYDTASKRYITLDGIAELIRNGEDVHVLDHETG
                     EDITAMTQAQIILEQERKLKGGLPRSVLTGLIQTGSDTLSHLRKALVPPAAWNAEVNA
                     EIERRLQRLVQQGALSEEEGLSLLDKLVEAGESSRDAERTSAQEIRHVLRRAGVPSRE
                     EVQELARQVEALAAELEKLSTARASRSKRAK"
     gene            complement(4644..5252)
                     /locus_tag="AC162G_08405"
     CDS             complement(4644..5252)
                     /locus_tag="AC162G_08405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0052851 - ferric-chelate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR5075873.1"
                     /translation="MKIGILGAGNVGGNLGRLWAARGHEIVFGVRDPKGDKIHALLGT
                     IGGSARAASIRDAAAFGEAVVLAVPWAAVQETLGQTDDWSGKILIDGTNRFTAGRAAS
                     GAEEIAGLAPGARVVKAFNTIGAELLASARFDTQSATMFICGDDAQAKSVVASLAEEL
                     GFEVVDAGPLSSAGMLESLARLWVSLARGGLGRNIAFKLLRR"
     gene            complement(5360..5989)
                     /locus_tag="AC162G_08410"
     CDS             complement(5360..5989)
                     /locus_tag="AC162G_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013982.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HIR5075874.1"
                     /translation="MKRLRRWLKRNPDRFMLLLIRQAEYTVQGMDGLMAYMRVPTEES
                     ATTVRRIEQEADEVRRILIDELNRTFVTPLDREDISALSRAIDDVLDYAFSTVDEMEI
                     LKVQPNPYLLRMASLLRDAAEEIRLAMLRLKDHPGVASDHAVRAKALENRMESVYREA
                     LAALFQGPQDVEHVVEMLKLREIYRHLSNAADRGDEAANVISDAVVKIT"
     gene            complement(6115..7101)
                     /locus_tag="AC162G_08415"
     CDS             complement(6115..7101)
                     /locus_tag="AC162G_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR5075875.1"
                     /translation="MLFGLPAAVVALIGLALIFDFLNGVHDSSNVVATMIASRAMGPR
                     RALAIAALAEFAGPFLFGVAVAETIGSGIVDEGAVTVPVLIAAVSAAIAWNLLTWFLG
                     IPSSASHALVGGLTGATVAAAGLDALKWGGLGKVFLSLFIAPPLGLLAGFTLMKAILF
                     LGRGASPRINVFFKRGQWVTALGLALSHGTNDAQKTMGLITLGLVVSGVLPEFRVPPW
                     VIALSAGALALGTSLGGWRLIRTLGARFYTIRPVHGFTTQTASASVILGASLLGGPVS
                     TSQVVSSSIVGAGSAERLSKVRWGVARQIGVAWLLTIPMSTLLAAGLYVLVP"
     gene            7226..8020
                     /gene="murI"
                     /locus_tag="AC162G_08420"
     CDS             7226..8020
                     /gene="murI"
                     /locus_tag="AC162G_08420"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010316791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR5075876.1"
                     /translation="MNDHPIGIFDSGVGGLSVWREIARQLPHEDTLYFADQTHIPYGP
                     RPMEQIRDFSEAIARFLLRRGCKAIVVACNTASAAALKGLRKQFPQVPFVGMEPAVKP
                     AVERTQTRVVGVLATPATFQGALFASVVERFANGVQLVNQVCPGLVERIEAGNFDTPE
                     TDAMLRDFLQPILDANADTIVLACTHYPFVVEPIRRIVGPGVNVIDPSPAIARQTGRV
                     LAERGLSAPESRAGRHVFCTSGERDAFCRVMGQLVNVNAQIEVVEV"
     gene            complement(8024..9262)
                     /locus_tag="AC162G_08425"
     CDS             complement(8024..9262)
                     /locus_tag="AC162G_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075877.1"
                     /translation="MAGLDYPVFPLDLSPDGRWLMVAFEIEYSTGRAAMAVIDTQGES
                     HWWMNTQANFFSPYFVTYPYLYPSLYPRWLPDGRLLWVNQVCKVFVWDGQNRRELSAP
                     EPVHPVYWVVYASKGIAFANTWDGGLWRVDLASERWEEVTTHRPPRLGRLGNAILAFN
                     GSYALAFQTDQSAAQMWRIPAEMGSFAEPLPDIQDWVDVPGHGGPSGPPSTHLADTPY
                     WLLNVGIALVVNERDGSIITAKDLHLPEGYYIIDYYASPDGRWLAVTITDDRQQPWEQ
                     PDMYIAPSTDLTAGRIVEGVSVAGWHIDAPAVILRDQATGALSVARLPLADATPRAPL
                     DNAMSPLVTLPNMVFAVDAASPARLLQFDLDGNLLSTLDLSSQYDLIRTGTGTAGRVF
                     LGVTRSQDGSHALVEWAVGP"
     gene            complement(9614..10606)
                     /locus_tag="AC162G_08430"
     CDS             complement(9614..10606)
                     /locus_tag="AC162G_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5075878.1"
                     /translation="MRVGLLVLMAALPLAACATPAVLGTVEPPIPTTALAPSIATVST
                     TVTPAAVTRAPTATPTPTRRPTATATLARITKTPPATPDIIGTLVATIQPRTYGTYLS
                     PDGEWRAEVIIYDCARIFGEDVNAYEQLKLTQASTGAEKIVDSQLQYCGGVGAFGLAG
                     LFWSPNSRYFYYTDARQGVPDGICSYWEPSSVSFDVVTGKTEYLGYGPRSPDGTKMAA
                     WRDRELVIWDINAGEVGRVSVRAMGFVDGPIAWSPDSQALAYVRTTDYCPPRGKSYIG
                     RVDLPGLRHTLLLESETPSFEGIQWDVLGQLRLFDEEGREWHYDFATEQLQPPF"
     gene            complement(10850..11182)
                     /locus_tag="AC162G_08435"
     CDS             complement(10850..11182)
                     /locus_tag="AC162G_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR5075879.1"
                     /translation="MQIATTIGPVTVLSVSGRIDAQTFPQLMAEADRRLEEGQIRLVL
                     DLSGVDYVSSRGLIALQTIASRAAARGGKAVLCGVEARVMQVLEMTGLHKRLGIFPDR
                     AAATASFE"
     gene            11620..13134
                     /locus_tag="AC162G_08440"
     CDS             11620..13134
                     /locus_tag="AC162G_08440"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5075880.1"
                     /translation="MSTSTIAFIYFFYGLAFFSMGLAITLEVGRATDSRLRRALPPLA
                     GFGWIHAIHEWLEMFIILQILPLHEAHPIIWEGPRIGLLAFSFLSLAAFGASLLSPNE
                     NIRRISLLVPLLLVAVWGFGALILRGQPLPDDELLDVIDVWTRYVLGIPAALLASAGL
                     IAQQRAFRRAGMVQFGRDSLWAAVAFAWYGLIGLLFTRQSALPPSTVINQELFLRLFG
                     FPVQLLRATAAGVAAFFVIRFLRSFEVEMRDKLAALQAAQLEDAQRREAQRGELLMRV
                     VTAQEAERQRVARELHDETGQALTAIGLGLRGVSTMLRADVEKAAQNLRHLEGLVAKS
                     LNELQRVIADLRPSHLDDLGLPAAVRWYASEVQNRAPLHIDVEVIGEPCPIPPAVKTA
                     LFRVVQEALTNVIKHAQAQHVKVYLSYADGKVGALVEDDGRGFDASRLEDTSRPSWGL
                     LGMEERAALLGGQFTLQSSPGQGTRVEVIIPVDVRDASERADEDRHDHTLTPGG"
     gene            13106..13756
                     /locus_tag="AC162G_08445"
     CDS             13106..13756
                     /locus_tag="AC162G_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5075881.1"
                     /translation="MTIRLLLVDDHVVVRSGLRMLLDNEPDVEIVGEAGTAREALDSV
                     PRLKPDIVLMDIGLPDKSGIDAAREIKRLRPETGIVALTIHEDEEYFFKMLEAGASGY
                     VPKRAAPEELLTAIRTAASGEVYLYPSMAKLLVKDYLDHEQEEKTGRMLDGLTEREQE
                     VLAHLADGASNEEIAETLNISPKTVARHRENIMRKLNLHSRSELVKYAIRKGIIQA"
     gene            14265..14609
                     /locus_tag="AC162G_08450"
     CDS             14265..14609
                     /locus_tag="AC162G_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075882.1"
                     /translation="MDVLEAIGVMLIGLAVRFGIPIVLTILIVMWLRRLDARWQLEAN
                     QTRPRAAGAAMPQVRCWEQRGCPPERRASCPVYARPDVPCWQVFRGADGRLHEACLDC
                     DVFRKAPVPAYA"
     gene            14646..15545
                     /locus_tag="AC162G_08455"
     CDS             14646..15545
                     /locus_tag="AC162G_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024827.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5075883.1"
                     /translation="MTRLFVGLIFALPMAMLPVALTQADAPGVPQESPPAQLACNACH
                     SDFQEAWEKGAHGRAINDPVFKEAWEEQGKPQQCLTCHTTGYDAEQGTWLADGVTCQA
                     CHSPVAAKHPEEPMAADRSAKMCGTCHNETFFEWQVSKHREVDLDCVGCHDSHGTTLK
                     AEDASAMCATCHRDRASNFAHTAHSQMGLTCADCHLGQLDGVAGEGHATRDHSFNVRL
                     EACNQCHAYQMHDPVQVHLDEPTPEPPDAMAAGETLSVSAAPEQVSPVGFAVVSGLVG
                     FAAGIVLAPWLERWYRRISRDEK"
     gene            15554..16327
                     /gene="dsrO"
                     /locus_tag="AC162G_08460"
     CDS             15554..16327
                     /gene="dsrO"
                     /locus_tag="AC162G_08460"
                     /inference="COORDINATES: protein motif:HMM:NF045797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrO"
                     /protein_id="HIR5075884.1"
                     /translation="MGTKTFKRRSFLKTAGIGVVAVAGLRMNERALSGPAPQGDSPHK
                     WAMVIDQAKCTGCGYCTLACRAHNDVPPSISWNRVIHAGKVKDQDVFLARPCMQCEHA
                     PCVEVCPVGASYYRPDGIVMMDYGKCIGCRYCLVACPYGGRAFNWEDFTGPNPAVPAW
                     GQPEVPRRPRGVVEKCSFCYHRIDRGLALGLKPGVDAEATPACVVACPVGARKFGDLN
                     DPESEVSKLLADHSYYRLREDLGTGPRVYYLPTREEVSG"
     gene            16324..17589
                     /gene="nrfD"
                     /locus_tag="AC162G_08465"
     CDS             16324..17589
                     /gene="nrfD"
                     /locus_tag="AC162G_08465"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR5075885.1"
                     /translation="MIAKHALKRPAFRLTRLQLWLFLLATLVVAGVIAGSVVFARGLV
                     VTNLNDLVPWGLWITIDLSSIALSAGAFLLCAAVYLLGLKQYQPVARTATFIGLIGYS
                     MAVLCLMLDIGRPDRFWHALVYWNTHSVLWEVTMCVALYFAVLALETAPILGQAAWLQ
                     RRLPRVAGLLGKAHTLAPYLAVVGLGLSLLHQSSLGATYGVLKARPIWYRPDLSVLFI
                     VSAMAAGPALTALASMLSARLTRRAHVDDRLLERLSFFIGWVLVGYLYFRFWDAFAMT
                     YTYEPGRTEGLNLITQGPLSFNFWIVEILLGAVIPIVILLNPRLRRNPLLRMLALALV
                     VVGVVAYRWDTNLAGQMVVMSYLPQDISARYTTYFPSLIEFVSGAGIVAYGLLAFTLG
                     VRFLNVVDHRHAQHHEAQPEAKPALAVSD"
     gene            complement(17712..19928)
                     /locus_tag="AC162G_08470"
     CDS             complement(17712..19928)
                     /locus_tag="AC162G_08470"
                     /inference="COORDINATES: protein motif:HMM:NF025875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5075886.1"
                     /translation="MTKRIIWIGMMLIGVGLLVVACTTPTPAPTLAPPPTTPPEPTGE
                     PVPVKLAPEVPYLANWEGSAHNAVDTEPFRHWDEDDPAEVPTTCAKCHSTAGYRDFLG
                     ADGSEAGKVDAAVPAQDAQGVQCVACHNPVTLRKTTVVFPSGVEITAGDDTRCMECHQ
                     GRESKVSVDQQITDFNATDPDAIPAPITDAQGNERRFGFRNVHYFAAAATLYGSMVHG
                     GYEYDGKTYDAKHTHVEGYATCTGCHDQHTLQVRVEQCATCHEGVASVEDLKNVRMVS
                     SNPDYDGDGDAEEGMYYEVQGLQEILLAEIQKYAQDVAGAEIQYDGATFPYFLGADGE
                     AYPNWTPRLLKAAYNYQVSQKDPGAFAHGNKYIVQLLYDSIEDLGGDVSGLARTDAAH
                     FAGDTLPFRDWDADDYTVPFRCVKCHTAQGMPTFVKNGGSVVVDGRGTTVTTGVGAMP
                     SSNGFMCTTCHNAEAFPERYAIAQVTFPSGKTLSFAQDADGKNVADDSNLCILCHQGR
                     ESSTSVDNYLSGKEDDTPDGNLSFKNIHYLSAGATLFGAEAAGGYQYAGKIYVGQNMH
                     AQPTGAVNKCKDCHDVHALEPKVEICQGCHGTDQVEEIRAPGDTVDYDGDGDTTEGTK
                     GEMDTLAEALYAQIQTYAETTAGTPIVYDTHSFPYFFVDADKNGEPDKDDEGASVRYN
                     AFTPRLMRAAYNYQYSQKDPGAFIHNPKYVFELLIDSIADLGGDVSAYTRPEAPAQ"
     gene            complement(20055..20402)
                     /locus_tag="AC162G_08475"
     CDS             complement(20055..20402)
                     /locus_tag="AC162G_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075887.1"
                     /translation="MDQARVVILSGHSLFTQGIASRLRQHSERLEIQIIDPHHADVSE
                     QVIAAQPSAVIIDASDPDAEMYCSIGELLVALPSLKIIRVDPQQPQIQVVTSEQRTAG
                     EVRDLIDLIEPPE"
     gene            complement(21071..22144)
                     /locus_tag="AC162G_08480"
     CDS             complement(21071..22144)
                     /locus_tag="AC162G_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HIR5075888.1"
                     /translation="MRDNPTKRRGLFRVHLLLLIGAALVVGACAPPYSLPAGPTPIPT
                     LMPATEPAISLGATAEPSFTVLSYPARLPSAALGQPLYQTHCASCHGADGKGVVPGAR
                     NFGDLDYMRGETPAFFYSAVTEGRGEMPSFRDSLTSDERWDVVFYVWRFSTDAETLAL
                     GQHLYDTNCAACHGADGIGAVLGAADFTDLRFVDDRAPRDFYLTVTQGKGSMPAWQGR
                     LSQDERWAVIDYLRAFSYDPALPGEAAAPPPTAVTVETACSSAYLSQSNPFAWDDATA
                     IAAGQAIYNEACSMCHAPDGSGALPGAPDFTAAETIDRLRSNSGELLCIVAEGRQAMP
                     GWKETLTAEQMWQVLTYMATLGE"
     gene            complement(22179..22685)
                     /locus_tag="AC162G_08485"
     CDS             complement(22179..22685)
                     /locus_tag="AC162G_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5075889.1"
                     /translation="MSPRLRRGLLIIGLVLIPFVVGLLLTYELIRIPFATNMADQPSV
                     DYQEGPRLLPPDGAVPVQGLSVIPEEFPVNLVPADEVSLQRGKILYGIHCQVCHGEQG
                     HGDGPLATYFDRTPQNLTGPQITAEFDGSVYLTIVQGFGQMPSLTENLTPRERWDVVN
                     YVRTLPAR"
     gene            complement(22682..23206)
                     /locus_tag="AC162G_08490"
     CDS             complement(22682..23206)
                     /locus_tag="AC162G_08490"
                     /inference="COORDINATES: protein motif:HMM:NF023249.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HIR5075890.1"
                     /translation="MTDTLTLMALFHDSVETAGAIDDLHTLGIPDEKMVVITGVPYPE
                     GALGRHREWLTLPYIVLAGAVGGLLFGLFLSAITPHLYRLDVGGHPAVSFPPTAIIVF
                     VFTMMATIVSTFLGVLWEMNFPRFGPTPYHRLVTDGHLAVLLEFPSELDAKVRQVLDS
                     HQAHHVGEPERHAL"
     gene            complement(23336..24742)
                     /gene="nrfD"
                     /locus_tag="AC162G_08495"
     CDS             complement(23336..24742)
                     /gene="nrfD"
                     /locus_tag="AC162G_08495"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR5075891.1"
                     /translation="MPNSQAPAPDSEFDERLLLAERTDREINQAVLQPMIQTRRGFWV
                     LVTILGALVAWGLYAWGYLIYWGIGSTGKNRPVYWTLFITTFVFWVGISHAGTMISAV
                     LRLLRADWRRPITRGAEAMTAFALMMAGLYPLIHLGRVWKFYWMIPYPNNRFMWPNFQ
                     SPLMWDLVAIFTYLIGSTLYLYLALIPDMAMARDHTGGWRTRLYRALALGWRGTEGEW
                     HKLHRALGIFSVAIIPIFLSVHSIVSWDFAVTIQPRWHSTIFAPYFVIGAIYSGLAAL
                     ASILVIVRRGMRLEEFLRAEHFNALGKLVMIAGMAWTYFWFAGFLVEWYGNEPVTREL
                     IREEVAGRLAPLFYLQMSANLIPIGLLVFKKVRTTPALLLAATLLVNVGMYTERALIV
                     IGSLQRNDLPFNWGTYRPTWVEVSIVVMTFAGFALLYTLFSRIVPYVPVWEIKEGRLR
                     YTLRRIGRLLVPTAAEIE"
     gene            complement(24735..>25061)
                     /locus_tag="AC162G_08500"
     CDS             complement(24735..>25061)
                     /locus_tag="AC162G_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5075892.1"
                     /translation="AFPEPLAEQLNPDVTVRSRGIMEKCTFCIQRIRRVEIDTRAEGR
                     EVADGEIQPACVQTCPPSALVFGDLNDPTSRVSQLARSHRSFRLLDELGTDPAVIYLK
                     GGETNA"
BASE COUNT         4704 a         7714 c         7962 g         4681 t
ORIGIN      
        1 ggagcaagcg attgtggcca ccgcccacaa gattgcacgc gcgtattatc atgtgctcaa
       61 gcatcgcaca ccgtttcggt cgctgagcgc cgacgaagac gagcagcaag tgcgccagcg
      121 cgaagtcgcg catctcaaaa agaaagcggc taaactcgga ttcacgattg tcgaaacgcc
      181 tgcctaagct caacgagacg caagtttctg agcaggagcg ccgttggcga cgaagcaatc
      241 tccgggccgg ccggggattg cttcgcaaac agatgctcgc aatgacattg ggctgcgtag
      301 gtacgtttct tagccaaaga gcacggaacg ccgcttggcg agacgcgcgt agatcatgtc
      361 ttgcacgata ttgcggactt gatcggtgag ctgcgagacg agcaccaggc tttgatccgc
      421 gtcgcggccg tagtggtcca tgggaatcgg ctcgccgatg tcgatggtcc acttggtcgg
      481 gaaggggatg aagccaatcg ggccaagcca gggccacgtc accgagatgg ggaagtaggg
      541 aaagccggtg atcttggcca tgacattgga cttcgccagc gagatgtagg tttcttcggc
      601 gcccacgatc gccacaggga tcatgggcgc gcacgttttg agcgccatct tgatgaagcc
      661 gccgcggccg aagcgcgcca gccggtagcg ttccttgaag agcttgccga cgcccttgta
      721 accctccgga aagacagcgg caagctcgtc ttgttccagc agacggatgc cgttgtcttc
      781 cgtcgccatc acctgcccca ttttcacgag gatgtcggag aagaagggca gcgtcgggaa
      841 ccacgtcgcg tacagattgc gcaccaggcg ctggttggga tgctcgagca tgacgccgat
      901 tcccaacatg gcgccatccc acggcaactg gccggagtga ttgaccacga gcagggcgcg
      961 cccttcagcc ggaatgtttt ccacgccggt catctggacg cgccaatatt tcttgtacat
     1021 gaactcgaag aagggccgca ccgcatccat gaactcgcga tccagccccc actcgtcggt
     1081 ttcgtactcg ccggtcaagc gccgccggaa cacatccgtc tggtattgca ccgtctggtt
     1141 gagcacggtc cacatgccct tccaggtatc cacgtcgagg tagtcttcgt tgatgaaccg
     1201 gcttaacgtg tcgcgaagtc tccgcgcgaa ccccggcgcg atcctgtcgg cgccttcctc
     1261 gatgacgtct atcacactgc cggtggttgc cggcgccgca ctgtaacccg gcgtgatggc
     1321 gcgcagccgc ttagccagct cgtccagctc gtgaatgagc tgctgccgca gcgagacctc
     1381 aacggtctcg acttccggct cggcctctgg gcgggtgtat tccacctccg gctcgggtat
     1441 ggcgagcgcc gtttcggcgg ccggttcggc cctcgcccgc gctggcttcc gcctggcccg
     1501 attcttcttc gacttttttt cggccattgg ggtctcctgt tttgcgactg ctcgacgaga
     1561 tgatcagcgc ttgacggtct tcgcccgtcg ctgtgtcctg catcgcgctg gcggagtgcg
     1621 cttgctgctt gccctggcgc gcgatcgagg cgctgacggc tcgctgtcca gagtgcgggc
     1681 gcggcggcgg cgctcgatcg tgtcgtgcag gcgttcctcg tcataggccc tcgcggccgc
     1741 ctcggggagg tatctgcgga gtcgctgctg agctgcgaat tcgcgcagcg cttcttcggc
     1801 cgtgtactga ggcacgaatc ctagctcggt gcgcatcttg cccaaatcgc ccacgcaggg
     1861 atagcgcagg tagtcgagcg cgatgggtgc gaaggccggc ccgagcagcg atacgccgag
     1921 ataggcgagg ggatgcaaga agggtatggg caacttgccc gccaggccca tgaccttcca
     1981 cagcggcatg acgccctccg ccgccacatt gaacacgccc ggcacatcgt tcaggatgac
     2041 gtggagcagc gcgcgcacga cgtcgtcctc gtgaatgact tgcatcatcg ggtcgaagcc
     2101 cagcagcgaa ggggcctgct cctcgcgcag gaagcgtgtg agcggcgtgt ccgctttcgg
     2161 tcccacgata tgcgcgaagc gcaagatagt cagcagaacg ccgggcgcct ggccgcgaaa
     2221 gccgttgcag aatgcctcga tctccacgag gtcgcgcgta tattcgtagc tctggctggc
     2281 ctgcaaggga taatcctcgg gcaggaaagc cgaattgccg ggattcgcgc cgtacaccat
     2341 ggtgctgctc ttcaagacga ccttgcgcac acccgcttcg gcgcacgcac ccagaagttt
     2401 catcgtgccc atcacgttga ggtcgaaggc caattcgctc gagcgagccg tctcgacgaa
     2461 ggtcacatgg cacaccgtat cgacgcgctc tgcttcgagc agcttcacga ccaggggatt
     2521 gcgcacgtcg gcctggatga agtcgagacc ttcgatcacc tcttcgggcg gtgtatcgac
     2581 cagcccgatc acgtgcaggt cggcgcgggc ggcgagctgg gcggcgaccc gactgcccca
     2641 gaatgcgttg acgccggtca ccaggatagc tcgcttctcg gtcatcgtgt ttgctcctgt
     2701 gacttccgaa gtctcggcta tttgaggacg agattgccgt agcccttctc tctcaaaagg
     2761 gcagggtaca ccttgtaaag cggctcgacg gccggcacaa gcttcaagat ctttggcacc
     2821 ggaccggagg cgacgatctc cttcttcgcc agggcggcga ccaggttgac tttgccgtgc
     2881 cagaaggcat gcgcgacgtc ggccttcatg gacatcgtga cgtccggcct cagattgcac
     2941 gcgccgtgga tgacgtccac gaaagcgtcc gcctgagtag gcttatcgcg ggcgttgatg
     3001 gtggtcatcg cctccggatc cgtgtagcga aactggatga tgatgccgga cttggcgatt
     3061 ttcgggccga ccttgggatc cttcttggcc cgatccatca gctctcccac acaatcgtag
     3121 aactgcgcgc tgtcgcgaaa ggtgctcacg gggattcctc ctgtgatggc gtgcgcatgc
     3181 ggcggatggg cgagtctctc tcgaggaaat actgtcgggc aacccgacgc cggaggcgat
     3241 cagggggatt acggctatca agctcggttg caggcgcttc gcaggtaaca taagtcgaga
     3301 accgggtgtt cttcgccttc caaattatac acgatcactg tggaaaagtc ttcggtgaaa
     3361 atgatgtttt ccctcttgac aaccatgacg caacgtgtta tcatgttcat gacgcagtgc
     3421 gttgtaagca agcgcccatt cgcaggatat atatcttttt cgcaaaccag gaggttgcac
     3481 gtgacactca aacagaagaa ggccgtcgaa accgaagcgc tcgcgccgga gaacggccac
     3541 gaatcgctgc tcgagccttt gcacaaggcc atgctggccg gcatcggcgc catggctatg
     3601 ggcgtcgatg cggttgacaa agtgacgggc cggttcatcg agcgcggcga gctcgtcgaa
     3661 aaggatgcgc agaagctctg gcggcagttc gccaaggagt ttcagaagcg ccagaagcgc
     3721 gccgagcgcg gagtgggcaa ggagctggag gacatgaggg aggcaatgga cattccctcg
     3781 aaagccgaca tcgacgcgct gggcgagaag atcgccgcgc tgagcacgaa ggttgacgcc
     3841 ctgaaggcgt cgtaaccgga tccccactct gcgaaactgc acagggagtg gtgaagcatt
     3901 tcgccactcc ccctgtgagt gtatccggga aggatgcgca tgctcgtcat caagcgctac
     3961 tccaatcgca agctgtacga cacggcatct aagcgataca tcaccctgga cggcattgcc
     4021 gagctgatcc gcaacggcga agacgtgcac gtcctcgatc acgagacggg cgaggacatc
     4081 acggcgatga cccaggccca gatcatcctg gagcaggaac ggaagctcaa gggcggcctg
     4141 ccgcgctcgg tgctcaccgg cctcatccag accggcagcg atacactgag ccatttgcgc
     4201 aaagcgttgg tcccacctgc cgcctggaac gccgaggtga acgccgagat cgagcggcgc
     4261 ttgcagcggc tcgttcagca gggcgccctg tccgaggaag agggactgag tcttctggac
     4321 aagctggtcg aggcgggtga atcgtccagg gatgccgagc ggacaagcgc gcaagagatc
     4381 aggcacgtgt tgaggagagc cggagtgcct tcgcgcgaag aggtgcagga gttggcgcgc
     4441 caggtggagg cgttagccgc cgagctggag aagctcagca cggccagggc ttctcgctcc
     4501 aagcgagcga agtgagcggc ggattcagga aatttgtgat acgcgccttc aggcgccagt
     4561 cgcaccacgc gttcggcaaa gtccatgggc gaaagcgtcg acggcatgat gggtagtata
     4621 ttcggggatt gggagcgagt caactaacgc cgcagcagct tgaaggcgat gttgcgtccc
     4681 agaccgccgc gtgcgagcga aacccatagc cgggcgagcg actccagcat gcccgcactc
     4741 gacagcggcc cggcgtcgac gacttcgaag ccaagctcct ctgcgagcga tgcgacgacc
     4801 gatttggcct gcgcgtcgtc gccgcagatg aacatcgtcg cgctttgcgt gtcgaagcgc
     4861 gcgctggcga gaagctccgc gccgatcgtg ttgaatgcct tgacgacacg cgcgcctgga
     4921 gcaagcccgg caatctcctc cgcgcccgat gccgcgcggc ccgccgtgaa gcggttcgtg
     4981 ccgtcgatca ggatcttgcc cgaccagtcg tccgtctgcc cgagcgtttc ctgcacggca
     5041 gcccacggca cggccagcac cactgcctcg ccgaaagcgg ctgcgtcccg gatgctcgcc
     5101 gctcgcgcgc tgccgccgat cgtgcccaac agcgcgtgaa ttttgtcgcc tttcggatcg
     5161 cgcacgccga agacaatctc gtgtccgcgc gcggcccaca gcctacccag gttgccgccg
     5221 acgttgcctg ctccgagaat gccgatcttc attttcgtga ccgctcgatt accgacccgg
     5281 ggatgaacgt gatccaggca gatgtccctg tcgctcgtca tccaccacgc atcacgcatc
     5341 acgttttacg tttcacgctc tacgttatct tcaccacggc atcgctgata acgttggccg
     5401 cctcgtcgcc gcgatcggcg gcgtttgaca ggtgacggta aatctcgcgc agcttcagca
     5461 tctcgacgac gtgctcgacg tcctgcgggc cttgaaacag cgcggccagc gcctcgcggt
     5521 aaacgctttc catgcggttc tcgagcgcct tggcccgcac ggcgtgatcg ctggcgacgc
     5581 cggggtggtc cttcaggcgc agcatcgcca gccggatctc ctcggcggcg tcgcgcagca
     5641 gggaggccat gcgcagcagg tacggattgg gctgcacctt caggatttcc atctcgtcta
     5701 cggtgctgaa cgcgtagtcg aggacgtcgt cgatcgcccg cgacagggcc gaaatgtctt
     5761 cccgatcgag cggtgtcacg aacgtgcggt tgagttcgtc gatcaggatg cgccgcacct
     5821 cgtcggcctc ttgttcgatg cggcgcaccg tcgtggccga ctcctcggtc ggcacgcgca
     5881 tgtacgccat cagtccatcc atgccctgta ccgtgtactc ggcctgccgg atgagcagta
     5941 gcatgaaccg atcggggttg cgtttaagcc agcgcctgag ccgtttcatc cgcgcctctc
     6001 tcaaaaatgg gcagtcaggc atccccgaat gccgcttgtg gcgcaggtcg cacccttcgc
     6061 caggctcagg gcaagttctc gggaagcgac ctactctttc caggcaattt cgtcttacgg
     6121 aaccagcacg tataggcctg ccgccagcag ggtcgacatc ggaatggtga gcagccaggc
     6181 gactccgatc tgtcgcgcca cgccccaacg caccttcgac aatcgctctg cactcccggc
     6241 gccgacgatc gacgacgaaa cgacctgtga cgtgctcacc ggtccgccca gcagagacgc
     6301 gccgaggatg accgacgcgg atgcggtctg tgtcgtgaag ccgtgcaccg ggcggatcgt
     6361 gtagaacctc gcgccgagtg tgcgaatcag gcgccagccg cccagcgacg tgccgagcgc
     6421 caatgcgccg gcgctcaggg caatcaccca cggcggcacc cgaaattccg gcagcacgcc
     6481 ggaaacgacc aggccgaggg tgatgaggcc catcgtcttc tgcgcgtcgt tcgtgccgtg
     6541 cgatagggcc agcccgagcg cggtgaccca ctgaccccgt ttgaagaaca cgttaatgcg
     6601 cggagacgcg cctcgcccca ggaacagaat cgccttcatc agagtgaagc cggccagcag
     6661 gccgagcggc ggcgcgatga agagcgatag aaagactttg cccaggccgc cccacttcag
     6721 tgcgtcgagg ccggccgcgg cgacggtcgc cccggtgagg ccgccgacca gcgcgtgcga
     6781 tgcgctcgac gggatgccca ggaaccaggt cagcagattc caggcgatgg ccgcgctcac
     6841 ggcggcgatc agcaccggca cggtcaccgc gccttcgtcg acgatgccgc tgccgatcgt
     6901 ctccgccacg gccacgccga acaggaacgg gccggcgaac tcggcgagcg cggcgattgc
     6961 cagcgcgcgc cgaggcccca tcgcgcgcga agcgatcatc gtcgcgacga cgttggacga
     7021 gtcgtgcacg ccgttgagaa agtcgaagat cagggcaagc ccgatcagcg cgacgacggc
     7081 ggccggcagc ccgaagagca taccgacagt ataccgcgtc tgatggagaa ttgcacacgg
     7141 ggtataataa acccatcacg cgacacgcat cacgcatcac gtatcacgtc tcacgtttca
     7201 cgcagcgaat gaaacctcgt acacgatgaa cgaccaccct atcggcatct tcgattccgg
     7261 cgtgggcggc ctgagcgtgt ggcgcgagat cgcccgccag ttgccgcacg aagacacgct
     7321 ctacttcgcg gatcagaccc acatcccgta cggcccgcgc ccgatggagc agatccgtga
     7381 tttttcggaa gccatcgcgc gctttctgct caggcgcggc tgcaaggcga tcgtcgtcgc
     7441 gtgcaacact gcctcggcag cagcgttgaa gggcctgcgc aagcagttcc cacaagttcc
     7501 ttttgtgggt atggagccgg ccgtcaagcc cgcggtggag agaactcaaa cgcgcgtcgt
     7561 cggcgtgctg gcgacgccgg ccacgtttca gggcgcgctg ttcgcgagcg tcgtcgagcg
     7621 cttcgccaac ggcgtgcagt tggtcaatca ggtttgtccg gggctggtcg agcgtatcga
     7681 ggcgggaaac ttcgacacgc ctgagacaga tgcgatgctg cgcgacttcc tccaaccgat
     7741 cctcgacgcg aacgctgaca ccattgtcct ggcctgcacg cactatccct tcgtcgtcga
     7801 gccgattcgc cgcatcgtcg ggccgggcgt gaacgtcatc gacccgtcgc cggcgatcgc
     7861 gcggcagacg ggtcgtgtgc tggccgaacg cggtttaagc gcgcccgagt cgcgcgccgg
     7921 acgacatgtg ttctgcacca gcggcgagcg cgatgccttt tgccgagtga tgggtcaact
     7981 ggtcaacgtc aatgctcaga tcgaggttgt cgaggtctga gccttatggc ccaaccgccc
     8041 actcgacgag cgcatggctg ccgtcctgac tgcgcgtcac gccaagaaac acccggcctg
     8101 ccgtgcccgt ccctgtgcgg atgaggtcgt attgcgagga aaggtcgagc gtgctcagca
     8161 ggttgccgtc aaggtcgaac tgcaggaggc gggccggcga cgcggcatcc accgcaaaca
     8221 ccatgttcgg taaggtgacg aggggcgaca tggcgttatc cagcggggca cgtggcgtcg
     8281 catcggcgag tggcagccgc gcgacggaca gcgcacccgt cgcctggtcg cggaggatga
     8341 cggcgggcgc atcaatgtgc cagccggcga cggagacccc ttcgactatc cgcccggcgg
     8401 tgaggtcagt agagggtgcg atgtacatgt cgggctgctc ccagggctgc tggcgatcat
     8461 cagtgatggt gacggccaac catcggccat ccggtgaggc ataatagtca atgatgtagt
     8521 agccctccgg caagtgcagg tccttggcgg tgatgatact gccatcccgc tcgttgacca
     8581 cgagggcgat gcccacattc agcagccagt agggggtgtc tgccaggtgg gtggacggtg
     8641 gcccggatgg accgccatgg ccgggaacgt ccacccaatc ctggatgtcc ggtagcggct
     8701 cggcgaacga gcccatctct gccggtatgc gccacatctg ggccgccgac tggtctgtct
     8761 ggaaggccag ggcgtaagac ccgttgaagg ccaggatagc gttccctaac ctgcccaatc
     8821 tcggcggcct gtgggtcgtc acttcctccc acctctcgga agccagatcg acccgccaca
     8881 aaccaccgtc ccaggtgttg gcaaaagcaa ttccctttga tgcatacacc acccaataca
     8941 ctggatgcac tggctcgggc gcgctcagtt cgcgccggtt ttgcccatcc cagacaaaaa
     9001 ccttgcacac ctggttcacc cacagtagcc gcccatcggg cagccagcgg ggatagaggg
     9061 agggatagag gtagggatag gtaacaaaat agggagagaa aaagtttgcc tgggtattca
     9121 tccaccaatg ggactcgccc tgggtatcaa tcaccgccat cgctgcgcgg cctgtgctgt
     9181 attcaatttc gaatgcgacc atcaaccacc tgccatccgg cgagagatca agggggaaga
     9241 cgggatagtc cagcccggcc acgtggcgct cccgttgtcc ctctatcgcc agtgactccc
     9301 agtgcaacgc ggacagatcg ggcagcggga cggcatcttg aaagcggtcg gaaggagggc
     9361 aggcagggcg ggtgggttca gggatgggtg tcgtggtcgg gaccttcgtg gccgttgccg
     9421 tgcgggtggg ttgcagcgtg accgtcgggg aggagaatac tgtgacggtg gggagtggct
     9481 ctctcgttgc agttggggag ccgcacccca cgaggcttgc accgcacaca agcaaaacga
     9541 caactgctct caaggaagcg catcgcgctc gtgctgatat atggctcatg tctagaacaa
     9601 gtagggtcga tgcttagaac ggcggttgaa gttgttcagt cgcaaaatcg tagtgccact
     9661 cccgcccttc ttcgtcgaac aacctgagtt ggcccaagac atcccattga atgccctcaa
     9721 aacttggcgt ctccgattct agcaaaagcg tatgtcgcaa gcctggcaaa tccacacgcc
     9781 caatgtagga tttgccgcgt ggcggacagt agtcggttgt gcgcacatag gccaacgcct
     9841 gactgtcggg tgaccaagcg atcggcccgt ccacaaatcc catggccctc accgaaactc
     9901 gcccaacctc acccgcgttg atgtcccaga tgaccagttc ccgatctcgc catgcggcca
     9961 tcttcgttcc atccggcgac cgggggccat aacccaggta ttctgtcttc ccggtcacca
    10021 catcaaagct gacgctggac ggttcccaat agctgcagat gccatctggc acaccttgac
    10081 gcgcgtctgt atagtaaaag taacggctgt tgggcgacca gaatagaccc gccagaccaa
    10141 aagcgcctac gccgccgcag tactgaagtt gactgtcgac aatcttttcc gcgccggtgc
    10201 tggcctgagt aagcttgagt tgttcataag cattcacgtc ttcgccaaag attcgggcgc
    10261 agtcataaat aatgacttca gctcgccatt cgccatcagg cgaaagatat gtgccgtaag
    10321 tccgtggctg gatcgtggca acaagcgtcc caataatatc gggtgtggcc ggcggtgtct
    10381 tcgtaattct tgcgagcgtg gcggttgcgg tcggcctccg agttggggta ggtgttgcag
    10441 taggtgctct ggtgacggct gccggcgtca ccgtcgtaga aacagtggca atggaaggtg
    10501 caagtgccgt cgttggtatg ggcggttcaa ccgttcctag cacggcgggc gtcgcacacg
    10561 cagccaacgg cagtgctgcc atcagcacca gtagcccgac tcgcatcatt gctcccgcgc
    10621 ttcatcgcgc ttcgatgacc tgaatgccat ctggtctgat cgggatcata tcgtgatcgt
    10681 gtgggaattg cctgacgctg ccgcgccgat ttgtctacga gccggagacg ccaggggaac
    10741 gcgcaagaca acctgcgcgg aagaagagag gaccaattgt cagaagggcg actgtttgtt
    10801 gtgcgtgact gaggatggtt tgcaacagac ctgcggaagt aatgtgtttt tactcgaaac
    10861 tggccgtcgc cgccgcgcga tcgggaaaaa tgcccagtct cttatgaagc ccggtcatct
    10921 ccagcacctg catgactcgc gcctcgaccc cgcacagcac ggccttgccg ccgcgcgccg
    10981 ccgcccggct ggcgatcgtc tgcagagcga tcaagccccg gctgctgacg tagtctactc
    11041 cgctcagatc gagcacgaga cgaatctggc cttcctccag tctccggtcg gcctcagcca
    11101 tcaattgagg gaaggtctgt gcatcgatac gcccgctcac gctcagaacg gtgaccgggc
    11161 cgatggttgt cgcgatttgc atacctctcc ctctctctgc ctcggaacgc cggcggcagt
    11221 cgcggttttc ccacgtgcgg tgcgtgcaat gttggggtgc ctcttctggc gcttatgaat
    11281 atataccgat cccggcaatg cgtcaatgga ccatgccgcc gtgcaggcac atgtccatca
    11341 actcggtatt gtctccggat ctctttgatc ctcgccatga gaaatagttg tgcaggtgtg
    11401 ctatactgcg cgcgggtata gattacgctg ttttcgctcc cgccgctgtc cgaagggcgt
    11461 gccaaatcca ccacgaacgg ggcggcagcg aaaagcgccg attgtgaccg cgttgagtat
    11521 aaagcatggc tttaagcaga cgaaaccgga caaaaatcac ttccccttat tgcggtccgg
    11581 ttgtacacta taccttgcgg tagaggcccg gagcgcagcg tgtcgacgtc gacgattgcc
    11641 ttcatctatt ttttctacgg cctggctttc ttcagcatgg gcctggccat cacgctcgag
    11701 gtgggacgtg cgaccgatag ccggctgcgc cgcgccttgc cgccgctggc cggcttcgga
    11761 tggatccacg ccatccatga gtggctggag atgtttatca tcctgcagat actgccgctg
    11821 catgaggctc atccgatcat ctgggaaggt ccgcgcatcg gactgctcgc gttttccttt
    11881 ctctcgcttg ccgcgttcgg cgcatcgctg ttgtcgccta acgagaacat tcgccgcatc
    11941 agcttgctgg tgccgctcct gctcgtggcc gtgtggggct tcggcgcact aatcttgcgg
    12001 ggccagccgc tgcccgatga tgagctgttg gatgtgatcg acgtgtggac ccgctacgtg
    12061 ctggggattc ccgctgcgct gctggcttcc gccggcctga ttgcgcagca gcgcgccttt
    12121 cggcgcgccg gcatggtgca gttcgggcgc gacagcctgt gggcagccgt cgcgttcgcc
    12181 tggtacggtc tcatcggcct gttattcacg cgccaaagcg cgctgccgcc ttcgacggtg
    12241 atcaaccaag agctattcct gcggttgttc ggcttcccgg ttcaactgct gcgcgcaacg
    12301 gcggcgggtg tggccgcctt cttcgtcatt cgcttcctgc gctcctttga ggttgagatg
    12361 cgggacaagc tcgccgcgct gcaggcggcg cagctcgagg atgcccagcg gcgcgaggcg
    12421 cagcgcggcg agctgctcat gcgcgtcgtc acggcgcagg aagccgagcg gcagcgcgtc
    12481 gcccgcgagc tgcacgacga gaccggccag gcgctgacgg ccatcggcct gggtctgcgt
    12541 ggcgtgtcca ccatgctgcg cgccgacgtg gagaaggccg cgcagaacct gcgccacttg
    12601 gagggcctgg tcgccaaatc gctcaacgag ctgcagcgcg tgatcgccga cctgcgccct
    12661 tctcacctcg acgacctggg cctgccggcg gccgtgcgct ggtatgcgag cgaggttcaa
    12721 aaccgcgctc cgctccacat cgacgtcgag gtgatcggcg agccgtgtcc gattccgccg
    12781 gccgtgaaga ctgcgctgtt ccgggtcgtg caggaggcgc tgaccaatgt gatcaaacac
    12841 gcgcaggccc agcacgtgaa ggtgtatctg tcatacgcag acggcaaggt cggcgcgctg
    12901 gtcgaggatg acgggcgagg cttcgacgcc tctcgcctgg aggataccag ccgaccgtcg
    12961 tggggactgc tgggaatgga ggaacgcgcg gcgctgctgg gagggcagtt cacgctgcag
    13021 tcaagcccgg gccagggcac gcgcgtggag gtgatcattc ctgttgatgt acgggacgca
    13081 tctgaaaggg ccgatgagga tagacatgac catacgctta ctcctggtgg atgatcacgt
    13141 cgtcgtgcgc tccgggctgc gcatgctgct ggacaatgag ccggacgtcg agatcgtcgg
    13201 cgaggccggc acggcgcgcg aggcgctaga cagtgtgccg cgcttgaagc cggacatcgt
    13261 gctgatggac atcggcctgc cggacaaatc cggcatcgac gccgcgcgcg agatcaagcg
    13321 gctgcggccc gagacaggca tcgtcgcgct cacgatccac gaagacgagg aatacttttt
    13381 caagatgctc gaagcgggcg ccagtgggta cgtgcccaag cgcgccgcgc ccgaggagct
    13441 gctcaccgcc attcgcaccg ccgccagcgg cgaagtgtat ctttacccgt cgatggcgaa
    13501 gctgctcgtc aaggactatc tggatcacga gcaggaggag aagaccggcc gcatgctcga
    13561 tgggctgacc gagcgcgagc aagaggtgct ggctcacctg gccgacggcg cgagcaacga
    13621 agagattgcc gagacgctca acatcagccc taagacggtc gcgcggcatc gcgaaaacat
    13681 catgcgcaag ctcaacttgc actcccgctc ggagctggtc aagtacgcca tccgcaaggg
    13741 gatcattcag gcttgaatga cgggagaaag ccaaagggat caaatttcaa agatcaaact
    13801 tcaaagatca aactccaaag ggcaaagcac aagggtccaa cttcaaatta agaggttcgt
    13861 tcagcccaga cacctgataa ctgacaactg acaactgata actgatgact gatcactgat
    13921 cagcccgacg tcgacgcacg ccgggctttt tttcgtcctg cgcgtgggtc gtttcaccca
    13981 tacggcgcga tccggcaccc cggccacgtg ggttactcgc ccaaccggcg ggcgcgcgcc
    14041 caggcgatcc tgcccatttc agaatcggtg acttgctgga tttacgcgcc tggcccggtg
    14101 atctatgcta caagcgacga gaaggaaagt cggaggtgtg agatggaaac catgctggca
    14161 gtgatcgttc tgacgattct gagactgggg ctgccgctcg gcctgctgct gctgctcggc
    14221 atactcgtgg agcggcgaca tagggtccga acgtgaggtg aactgtggac gtgctcgagg
    14281 cgatcggtgt gatgctcatt ggcctggccg tgcgatttgg cataccgatc gttctcacga
    14341 tcctgatcgt gatgtggctg cgccggctcg acgcccgctg gcagctcgaa gcaaaccaga
    14401 cacgtccgcg agcggccggc gccgctatgc cacaggtgcg ctgctgggag cagcgtggat
    14461 gcccacccga gcgccgggcg tcgtgccctg tctacgcccg gccggatgtg ccgtgttggc
    14521 aggtcttccg cggcgcagat ggccgcctgc atgaggcttg cctggactgc gatgtcttca
    14581 ggaaggcgcc ggttccggct tacgcataac ctgactgcga gggaagaacg atgatcagac
    14641 attggatgac tcgactgttc gtcggcctga tcttcgcgct gccgatggcc atgctgccgg
    14701 tggcgctgac gcaggccgac gcgcccggcg tgccgcagga atcaccgcct gcgcagttgg
    14761 cgtgtaatgc ctgtcactct gatttccagg aagcctggga aaaaggcgcg cacggccgcg
    14821 cgataaacga cccggttttc aaggaggcct gggaagagca gggcaagccg cagcagtgcc
    14881 tcacctgcca cacgaccggc tacgacgcgg agcaaggaac ctggcttgcc gacggagtga
    14941 cgtgccaggc ctgccatagc cccgtggccg ccaaacaccc tgaggagccg atggctgccg
    15001 accgctcggc caagatgtgc ggcacgtgcc acaacgagac cttcttcgaa tggcaggtga
    15061 gcaagcatcg cgaagtcgac ctggactgcg taggctgcca cgattcgcac ggcacgacgt
    15121 tgaaggccga agacgcctcg gccatgtgcg ccacgtgcca ccgcgatcga gcctccaatt
    15181 tcgcccacac cgcgcacagc cagatgggcc tgacctgcgc ggactgccat ctcggccagc
    15241 tcgacggcgt agccggcgaa ggtcacgcca cgcgcgatca cagcttcaac gtgaggctgg
    15301 aggcgtgcaa ccagtgccac gcctaccaga tgcacgaccc ggtccaagtg cacctcgacg
    15361 agccgacgcc tgaaccgccg gatgcgatgg ctgccggaga gacgctcagc gtgtccgcag
    15421 cacccgagca ggtcagccca gtcggattcg ccgtcgtctc cggcctggtc ggcttcgccg
    15481 ccggtatcgt gctggcgcca tggctggagc gctggtaccg gcgcatcagc cgcgacgaga
    15541 agtgaggcgc tccatgggaa caaagacatt caagcggcgc agcttcctga aaacggcggg
    15601 catcggggtc gtggccgtcg ccggcctgcg catgaacgag cgcgcgctct caggcccggc
    15661 gccacagggt gacagcccgc acaagtgggc gatggtcatc gatcaggcga agtgcaccgg
    15721 atgcggatac tgcacgctcg cctgccgcgc gcacaacgac gtgcctccct cgatctcgtg
    15781 gaaccgcgtg atccacgccg gcaaggtcaa ggaccaggac gtctttcttg cgcgtccgtg
    15841 catgcagtgc gagcacgcgc cgtgcgtcga agtttgcccg gtcggggcca gctactaccg
    15901 gccggacggc atcgtcatga tggactacgg caagtgcatc ggctgccgtt actgcctggt
    15961 ggcctgcccg tacggtgggc gcgccttcaa ctgggaagat ttcaccgggc ctaaccccgc
    16021 cgttccggcg tggggacagc cggaagtgcc gcgccggccg cgcggcgtgg tcgagaagtg
    16081 ctcgttctgc taccaccgca tcgatcgcgg gctggcgctc ggattgaagc cgggcgtcga
    16141 cgcggaggcc acgccggcct gcgtggtggc ctgcccggtc ggcgcgcgaa agttcggcga
    16201 cctgaacgat cctgaatcgg aagtgagcaa gctgctcgcc gatcattcct actatcggct
    16261 gcgcgaagac ctggggacgg gtccgcgcgt gtactatctg cccacgcgtg aggaggtgtc
    16321 cggatgatcg cgaagcacgc gctcaaacgc ccggcgtttc ggcttacgcg gctgcaatta
    16381 tggctcttcc tgctggcgac gctcgtcgtg gccggcgtga tcgcaggcag tgttgtgttt
    16441 gcgcgcggcc tggtcgtgac gaatctgaac gaccttgtgc cgtggggcct gtggatcacg
    16501 atcgatctgt cctcgatcgc cctgagcgcg ggcgcattcc tgctgtgcgc ggcagtgtat
    16561 ctgctagggc tgaagcaata ccagcccgtc gcgcgcacgg ccactttcat cggcctgatc
    16621 ggctacagca tggccgtgtt atgcctgatg cttgacattg gccgtccgga tcgcttctgg
    16681 cacgcgctgg tgtactggaa cacgcactcg gtgctgtggg aagtcacgat gtgcgtggcg
    16741 ctgtacttcg ccgtgctcgc gctggagacc gcgccgatcc tcggccaggc tgcctggctg
    16801 cagcgccgcc tgccgcgtgt ggccggcctg ctcggcaagg cgcacactct cgcgccgtac
    16861 ctggccgtcg ttgggctggg attgtcactg ctgcaccagt cgtcgctcgg cgcgacgtac
    16921 ggcgtgctca aggcccggcc gatctggtac cgccccgatc tgtcggtgct gttcatcgtc
    16981 tcggcgatgg cggccgggcc ggcgctcacg gcgctggctt cgatgctgtc cgcgcgtctc
    17041 acgcgccgcg cccacgtcga cgatcgcctg ctcgagcggc tgtcgttctt catcggctgg
    17101 gtgctggtcg gctacctgta cttccgcttc tgggacgcgt tcgccatgac ctacacctac
    17161 gagccggggc gcaccgaagg gctgaacctg atcacgcagg ggccgctgtc gttcaacttc
    17221 tggatcgtcg agattctgtt gggcgcagtc atcccgatcg tgatcctgct caacccgcgc
    17281 ctgcgcagga acccgctgct gcgtatgctc gcactcgcgc tcgtcgtcgt cggggtcgtg
    17341 gcctatcgct gggacacgaa cctggccggc cagatggtcg tgatgagcta cctgccgcag
    17401 gacatctcgg cgcgctacac cacctatttc ccatctctga ttgagttcgt cagcggggca
    17461 gggatcgtgg cgtacgggct gctggccttc acgctcggcg tgcgcttctt gaacgtggtc
    17521 gatcatcggc acgcgcaaca tcacgaagcg cagcccgagg caaagccggc gctcgcagtt
    17581 tcagattaat agaagacctg ccaggtctca gagacctggc aggtctttgt gcattcgaaa
    17641 aagaaccccg gtttcttcac gccctccgct acgcttcgga ggccttcggg aaaccgggtt
    17701 tcttatcgtg attactgcgc cggtgcttcg gggcgggtgt aagccgatac gtcgccgccc
    17761 aggtccgcga tcgagtcgat caacagctcg aaaacgtact tcgggttgtg gatgaaggcg
    17821 cccggatcct tctgggaata ctggtagttg taggcggctc tcatcagacg aggcgtgaag
    17881 gcattgtagc gcacagaagc gccctcgtcg tccttgtcag gctcgccatt cttgtcggca
    17941 tccacgaaga agtacgggaa gctgtgcgta tcgtacacga tcggcgtgcc ggccgtggtc
    18001 tcggcgtacg tctggatctg cgcatagagg gcttcggcca gggtatccat ctcgcccttg
    18061 gtgccttcgg tggtgtcgcc atcaccgtcg tagtcgacgg tgtcacccgg agcgcggatt
    18121 tcctcgacct ggtcggtgcc gtggcagccc tggcagatct caaccttcgg ctccagcgca
    18181 tggacatcgt ggcagtcctt gcacttgttc acggcgcccg tcggctgcgc gtgcatgttc
    18241 tggccgacgt atatcttgcc ggcatactgg taaccgcccg ccgcttcagc gccgaacaag
    18301 gtcgcgccag cgctcaggta gtggatgttc ttgaagctca ggttcccgtc cggggtgtcg
    18361 tcttccttgc cgctcaggta gttgtcgacg ctggtggagg actcgcggcc ctggtgacac
    18421 aggatgcaca ggttggagtc gtcggcaacg ttcttgccgt cggcgtcctg ggcgaaggac
    18481 agggtcttgc cgctcgggaa ggtcacctga gcgatagcat agcgctcggg gaaggcttcc
    18541 gcgttgtggc aggtggtgca catgaagccg ttgctggagg gcatggcgcc aacgccggtg
    18601 gtcacagtcg taccgcggcc atcgacgacg accgagccac cgttcttgac gaaggtgggc
    18661 atgccttgag ccgtgtggca cttcacgcag cggaacggca ccgtgtagtc gtcagcatcc
    18721 caatcgcgga acggcagggt gtcgccggcg aagtgggcag catccgtgcg ggccaggccg
    18781 ctcacgtcac cgcccaggtc ttcgatcgag tcgtacagca gttggacgat gtacttgttg
    18841 ccgtgcgcga acgcgccggg atccttctga gagacctggt agttgtaggc ggccttgagc
    18901 aggcgcggcg tccagttcgg ataggcctca ccatccgcgc ccaggaagta aggaaaggta
    18961 gcgccatcgt actggatctc ggcgccagcc acatcctggg cgtacttctg gatctcggcc
    19021 agcaggattt cctgcagacc ctggacctcg tagtacatgc cctcttccgc gtccccatcg
    19081 ccgtcgtagt ccgggttgga gctgaccatg cggacgttct tcaggtcttc cacagaggca
    19141 acgccttcgt ggcacgtcgc gcactgttca accctcacct gaagcgtgtg ctggtcgtgg
    19201 cagccggtgc aggtcgcata gccttcgacg tgcgtgtgct tggcgtcgta ggtcttgcca
    19261 tcatactcgt aaccgccatg caccatggag ccgtacaggg tagcggcagc cgcaaagtag
    19321 tgaacgttgc ggaaaccaaa cctgcgctcg ttgccctgcg cgtccgtgat gggcgcggga
    19381 atggcatcgg ggtcggtcgc attgaagtcg gtgatctgct ggtctacact gaccttggac
    19441 tcgcggccct ggtggcactc catacaccgc gtgtcatcac cggctgtgat ctccacaccg
    19501 gacgggaaca cgacggtggt cttcctcaag gtgaccgggt tgtggcaggc gacgcactgg
    19561 acgccctgag catcctgagc gggcacggcg gcatccacct tgccggcttc agagccgtcg
    19621 gcgcccagga agtcgcggta gcccgcggtg gaatgacact tggcgcaggt ggtcggcact
    19681 tcggccgggt catcctcgtc ccaatggcgg aagggttcgg tgtcgacggc gttgtgggcg
    19741 gaaccttccc aattcgccag gtaggggact tccggggcca acttgaccgg caccggctcg
    19801 cctgtcggct ccggcggggt cgtcggcggg ggcgccagcg tgggcgccgg cgtcggcgtt
    19861 gtgcaggcga ccaccagcag cccgacaccg atcagcatca tccctatcca tatgattcgt
    19921 ttggtcatag ttcatctctc cgattcgagt agtgcagcga tttgcgccga cgtgaagtgt
    19981 acgaggggtc cattttcgtt ttgcgccttc atcctccttg ccagatggct tgagcagcgg
    20041 gcacctgtct tcgtttattc gggcggctcg atcaagtcga tgagatcgcg cacttcgcct
    20101 gccgtgcgct gctcgctggt gaccacctga atctgcggct gctgtggatc gacgcgaata
    20161 attttcagcg acggcagcgc gacgagcaac tcgcctatcg agcaatacat ctctgcatcc
    20221 ggatcgctcg cgtcgatgat gacagccgaa ggttgcgcgg cgatgacctg ctcgctgacg
    20281 tctgcgtggt gggggtcgat gatctgaatc tccaggcgct ctgaatgttg ccgtagccga
    20341 cttgcaatac cctgtgtaaa taacgagtga ccggacagga tgaccacgcg agcctgatcc
    20401 acgacagacc tcgaagcgat ggaatgctcc accactccgt gaagaatagc gcaagatgag
    20461 cgccatcagc aacactcgtt agttcaaatc ttgatacaaa tgagggtagg cgaaaagaca
    20521 ccatagccta cccgttcggg tagggtgaca atagtttgcg acttctaacc aagtcttaca
    20581 caattattac ccacaaaccg tgccaaatcg actaaaatgt cggtgtaatg tgtagaatgt
    20641 cgctgtaccg tgtgccggcg gatgctcgcc agtgtgttca tccacccacc cagagcatcc
    20701 gccgtgttca tttttagcaa catacgggcg gtgcaaccat gcccacacat ggcccgagcg
    20761 agccgatctc cggcccaccc ggcgcgagga gccatgatga cctattgcaa atactgcggc
    20821 cttagcaagt gctgctgccc ttctgccgaa gaactgaagc agatggtcaa ggtgctcgag
    20881 ggtcttgaag agggttgtgt catagacacg caatcgatgg gccgccctta tgcgctcatg
    20941 ccagtcggtc agcacgcgga tcgccccggt cgtgtagcac gagacgcagg actccgacga
    21001 cctgcggagc gcacaagggc ggctcaaacc aagcggggct agggggatgg ggtgaggaag
    21061 tcttgtctgc tcattcgccc agcgtcgcca tgtaggtcag cacctgccac atttgctcgg
    21121 cggtgagcgt ctctttccag ccaggcatgg cttgccggcc ctcggcgacg atacacagca
    21181 gttccccact gttagagcga agcctatcaa tcgtttcagc ggccgtgaaa tcaggcgcgc
    21241 ccggcagtgc acccgacccg tccggagcat ggcacatcga gcaggcctcg ttgtagatcg
    21301 cctggccggc tgcgatggct gtcgcatcgt cccaggcgaa gggattggac tgcgagagat
    21361 aggctgagct acaggcagtc tcgacagtga cggccgtcgg cggaggtgcg gcggcctccc
    21421 ccggaagagc cgggtcgtag gagaacgcgc gcaggtagtc aatcacggcc cagcgttcgt
    21481 cctgcgagag gcgtccctgc caggccggca tagagccttt gccctgtgtg acggtcaggt
    21541 agaaatcgcg gggagcgcgg tcgtccacga accgcaggtc ggtgaagtcg gccgcgccga
    21601 ggactgcgcc gatgccgtca gctccgtggc aagcagcgca gttggtgtca taaagatgct
    21661 gaccgagagc gagggtttcg gcgtcggtgg agaagcgcca cacgtagaac accacgtccc
    21721 agcgctcatc cgacgtcaga ctatctcgaa atgacggcat ctcgccacgc ccttcggtga
    21781 cggcagagta gaagaaagcc ggggtctcgc cgcgcatgta gtccaggtcg ccgaaattgc
    21841 gtgccccggg gacgactccc ttgccgtctg cgccgtggca actggcgcaa tgggtttggt
    21901 agagcggctg gccaagcgct gccgagggca accgcgccgg ataactcaga acggtgaacg
    21961 aaggctctgc tgtcgcgccg agggaaatgg ccggttcggt ggccggcatc agcgtaggta
    22021 tgggcgtggg accggcgggc aggctgtatg gcggagcgca ggcgcccacg accaaggcag
    22081 caccgatgag caggagcaga tgcacgcgga atagtccccg tcttttggtg ggattgtctc
    22141 tcattggaat tctctcaggg atggtcacac cgaacgcttc accttgccgg cagagtacgc
    22201 acgtaattga cgacatccca gcgctcgcgc ggcgtcaggt tctcagtcaa cgagggcatc
    22261 tggccaaagc cctgaacaat cgtcagatag actgagccgt cgaattccgc agtgatttgc
    22321 gggccggtca ggttttgcgg tgttcgatcg aagtaagtgg ccagcggccc atcaccgtgc
    22381 ccttgttcgc cgtgacagac ttgacaatgg atgccgtaca ggattttgcc gcgctgcaac
    22441 gaaacctcgt cggccgggac cagattgaca gggaattctt cggggatcac cgatagtccc
    22501 tgcacgggga ctgcgccgtc gggtggcaga agccgagggc cttcctgata gtctaccgag
    22561 ggctgatcgg ccatgttcgt ggcaaacggg atcctgatga gttcgtatgt gagcaacagc
    22621 ccgacgacga agggtatcag gaccaggcca ataattaaca gaccacggcg caagcgcggg
    22681 ctcatagggc atgcctttcc ggttcgccca cgtgatgcgc ctgatgggag tcgagcacct
    22741 ggcgcacttt ggcgtcgagc tcggacggga attctagcaa tacggccagg tgtccatcgg
    22801 tgacaagcct gtggtaaggc gtcgggccaa atcgagggaa gttcatttcc cacaggacgc
    22861 ccaaaaacgt cgagacgatg gtggccatca tggtgaagac gaagacgatg atcgcagtgg
    22921 gcggaaagct gacagcagga tgtccaccta catccaggcg gtacaggtgc ggcgtgatgg
    22981 ccgacaggaa caggccgaac agcagcccgc cgaccgctcc ggccagcacg atgtacggca
    23041 gcgtcagcca ctcgcgatgc cgccccagcg cgccctcggg gtacggcacg ccggtgatca
    23101 cgaccatctt ctcgtccgga atgccgagtg tgtgcaggtc gtcaatcgcc ccggctgtct
    23161 cgacactatc atggaagagg gccatcagtg tcagtgtgtc agtcatcagt tatcagtcat
    23221 cagttttcag tcgtcagtta tcagtgatca gttatcagtt gtcagttatc agtcatcagt
    23281 tgtccagtac tgatgactga ccactgatta ctgatcactg gtcactgatt accggtcact
    23341 cgatctctgc cgccgtcggc accagcagcc gcccgatgcg acgcagggta tagcgcaggc
    23401 ggccttcctt gatctcccat acgggcacat aagggacgat gcgcgagaac agcgtgtaga
    23461 gtagagcgaa cccggcgaaa gtcatgacga cgatgctcac ttccacccag gtcggacgat
    23521 acgtgcccca gttgaagggc agatcgttac gctgcaggct accgatgaca atgagggcgc
    23581 gctcggtgta catacccacg ttgacgagca gcgtcgccgc caatagcagc gccggcgtcg
    23641 tgcgcacctt cttgaaaacc aaaagcccaa tcgggatcag gttggcgctc atttgcagat
    23701 agaacagcgg cgccaggcgg ccggcgacct cctcgcggat cagctcgcgc gtgaccggct
    23761 cgttgccata ccactccacc agaaagccgg caaaccagaa gtaagtccaa gccatgccgg
    23821 cgatcatgac cagtttgcct agcgcgttaa aatgctccgc gcgcaggaac tcctccagtc
    23881 gcatcccccg gcgcacgatc accaggatgc tggccagcgc cgccaggcca gagtagatgg
    23941 caccgatgac aaagtagggc gcaaagatgg tgctatgcca gcgcggctga atggtcaccg
    24001 caaaatccca cgacacgatc gagtggacgg agaggaagat cgggatgatc gccaccgaga
    24061 agatgcccag cgcacgatgg agtttatgcc actcgccctc cgtgccgcgc cagccaagcg
    24121 ccagcgcccg atacagcctg gtgcgccagc ctccggtatg gtcgcgcgcc atcgccatgt
    24181 cggggatcag cgccaggtac agatacaggg tcgagccgat caggtaggtg aaaatggcga
    24241 ccaggtccca catgagcggc gactgaaagt tgggccacat gaagcggttg ttcgggtagg
    24301 ggatcatcca gtagaacttc catacccggc ccaggtggat aagcggatac agtccggcca
    24361 tcatcaatgc gaaggccgtc atggcctccg cgccacgggt gatgggtcgg cgccagtcgg
    24421 ctctgagaag tcgcagcacg gccgagatca tcgtcccggc atggctgatg cctacccaga
    24481 agacgaaggt ggtgatgaag agcgtccagt acaccggccg gtttttcccg gtgctgccaa
    24541 tgccccaata gatgaggtag ccccaggcgt acaaccccca ggcaaccaac gcgcccagga
    24601 tagtcaccag cacccagaac ccccgcctgg tctggatcat cggctggagc accgcttgat
    24661 tgatctcgcg gtccgtgcgt tcggccagca gcagccgctc gtcaaattcg gagtcgggag
    24721 ctggggcttg ggagttaggc atttgtctcg ccccccttga ggtagatcac cgcagggtcg
    24781 gtgcctagtt cgtccaggag acgaaaggat cgatggctgc gggccagttg gctaacccgg
    24841 ctagtcgggt cattcaggtc accgaagacc agcgctgacg gtgggcaagt ttggacgcag
    24901 gcgggctgaa tctcgccatc agccacctcg cgtccttcag cccgggtatc gatctcgacg
    24961 cggcggatgc gctggatgca gaaggtgcac ttctccatga ttccgcgcga ccggacggtc
    25021 acgtccgggt tgagttgctc ggcgagcggc tcagggaagg c
//
LOCUS       DBNHXB010000208         8042 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4073491, whole genome shotgun sequence.
ACCESSION   DBNHXB010000208 DBNHXB010000000
VERSION     DBNHXB010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8042)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4073491"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="AC162G_08505"
     CDS             <1..183
                     /locus_tag="AC162G_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075893.1"
                     /translation="SGAERLCGRGDFLAVSGAGVIRFQAAFAAPEEIARLVELMLGTE
                     GHKVIWEQLQPALEPV"
     gene            264..608
                     /locus_tag="AC162G_08510"
     CDS             264..608
                     /locus_tag="AC162G_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075894.1"
                     /translation="MSKNEMKNGGIVAVGFVSLVIAVAIIVGSRLSSETVAALAGAAC
                     GAGLAAPLGIAIGMVVGSSRSRSTATPVPPPQVVVIPPQNPPPSHAPLYPPTLEPLVP
                     RSRPFTIIGEDD"
     gene            complement(673..1323)
                     /locus_tag="AC162G_08515"
     CDS             complement(673..1323)
                     /locus_tag="AC162G_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5075895.1"
                     /translation="MPTILLIESARSKSSYAPYLERRKYDVIRASTVKQALACARDEA
                     PALIVLDSTSSRIDGPRLCRAIRARVDGIAILLLVRSNVEVDATSGADFVLRLPFTPR
                     KLLNRVARMLPSGEADVLTLDDVMLNVATRCVRLGKKEHQLTPKQSQLLEIFMRQPNE
                     VITRRQLMKAVWNTDYMGDTRTLDVHIRWLRQIIEVDAGRPRRLVTVRNVGYKLKA"
     gene            complement(1439..1651)
                     /locus_tag="AC162G_08520"
     CDS             complement(1439..1651)
                     /locus_tag="AC162G_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075896.1"
                     /translation="MALKRAVVIIVGLAIGAAVTFGIVYFLQISSSDPLLLQKFGALN
                     FILASTMFGAIAIIYLDKFLKTQILK"
     gene            complement(1727..3370)
                     /gene="murJ"
                     /locus_tag="AC162G_08525"
     CDS             complement(1727..3370)
                     /gene="murJ"
                     /locus_tag="AC162G_08525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR5075897.1"
                     /translation="MMTDSQSAEATLTRNRRIAVAALLVGAGFLASKVTGILDDLILA
                     RIIGPGRELDAYYAAFSLPDLLFTLVAGGALASAFIPVLSGLIARDRAASWRLTSAVV
                     SLAFLVTLCFAIGLALFAPWIADVLYGCHQGSAANEQCFTPEQVVLTANLMRVILIST
                     AIFSVSAVVMSTLQAHQHFLLPALAPILYNLGILAGVILLAPSIGVWGPAIGVVVGAV
                     LHLLVQVPGLARYGARWSPVLSLNDVLLRRVLKLLVPRVAGLGVVQLTFVMTTSLASQ
                     LSPGSVTVLNYAWRVMQLPETVIATAVATAVFPTLSELAASGKIAELRATISSTLRTI
                     LALTVPAAVGLIVLGRPFVRVLFEGGLFQASATDAVVWALQAYALGLIAHSMLEVCAR
                     IFYAQQDTWTPLYVAAGAMIIVIAASLILREAFGHAGLALANSIGVSLEVLALLLIAG
                     RRLRGIEGRRIAASLARFVAASVVMGLAIAVSQVVFAKWPLPDGVSVSLTLRSALEIG
                     IPFSIAAGVGMLVYVVTALAMGSEELRALPRVVLRRHAA"
     gene            complement(3385..4764)
                     /locus_tag="AC162G_08530"
     CDS             complement(3385..4764)
                     /locus_tag="AC162G_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024919.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5075898.1"
                     /translation="MKTRYAGRWAFFAAAIGAVMLMAGVFAVRSLQPRYQASLINRLP
                     SFLRPAARSLILPDYPDVVPTPAAQITMDASELLAPRQTKTPAPSPTPASSRTPTPTL
                     TSQTPEPTDPPGQTDILAQTQPAARLTLTPVIPPGPRQAARSTPASLESTDVLLTGFR
                     HIYQTWNNCGPATLSMNLSFYGWGGGQADAAKFLKPDPEDKNVSPHEMTAFARSLGFE
                     AVMRVDGSIPLVKEFLRAGIPVLIEKGFEPDPQSGWEGHYGLVVGYDDSKNAFVLMDS
                     YTGPNQSMTYDTLDWYWSHFNRTFIIVHTPDQAGTVMQILGADADPTENLTGALATAQ
                     AEATTNPSNPFAWFNVGSNFVALGDYESAAAAYDQARSLGLPWRMTWYQFGIFEAYYR
                     TGRFDDVLALADATLATTPYVEELWYYRGLVRQARGEPDAAREQFRQALHYNPNFTAA
                     AEALAVLGQ"
     gene            4902..5399
                     /locus_tag="AC162G_08535"
     CDS             4902..5399
                     /locus_tag="AC162G_08535"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106130 - purine phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR5075899.1"
                     /translation="MMRQEVVTWTDLEMLVDTLIPQLYGVFDALLLITRGGIVPGGMI
                     AEALDIKYVLTAAVRFPEVDQKLLAWPTFLQFPEDDLLRNRRVLVVDDVWGSGRTINS
                     VRGRIEAAGGKPEVAVLHYKPGESLFTTKPEYYAAVTDAFIIYPWEKGRGTQGIFRRL
                     EPSLI"
     gene            5736..>8042
                     /locus_tag="AC162G_08540"
     CDS             5736..>8042
                     /locus_tag="AC162G_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR5075900.1"
                     /translation="MQSREERLDLVLQGTNDGIWDWDMRTGEVYFSPRWKSMLGYADD
                     EVANRKDEWNRLLHPNDLERALAAVQDYLDGRTTTYQLEHRLRHKDGTYHWVLARGIA
                     LRDEAGRAYRMVGSHTDITDRKHAEQALLESETNLRSLMENARDFAVYRIAVDPSNPY
                     LARVVVASPSLKEVFGIPDLHDFTTWFTPIHPEDLSRIVEANRRSLEMGVPFDQQLRF
                     FNAERGSWVWAHVRSSPVFDTDGRLTHFNGLVVDISEQKRVEDEMRRQDELLQKRLAF
                     ENLISRISTEFINLGPNEIDLGIRHALQAIGEFAGADRSYIFLVSGDGTRMANAHKWC
                     AESIEPAIGRVAEVSAESFPWFIERMNRLEIVQIPRVADLPPEAGAEKQEFQSQSTQS
                     LLAVPMVYRGSLVGFVGFDSVRSEKAWDEDSIALLRIVGEIFVNALEHKRAQAIQAGQ
                     RQFLELLATGGDFSGTLHTLVRLIEEQWPGMLGLVLLLDVDGRHLHIGASISLPEDYV
                     QSIEGLEIGPMVGSCGTACYRRERVIVEDILTDPRWDGLRDLAVKYGLRACWSEPVFS
                     AGGQVVGTFAMYYRHPRAPTAAELRAIEMAAHLVGIAIEHKRAQKALQAAYQTLEQRI
                     EERTRELAALNAIAAVVSRSLDLKEVLSNALDKTMEVIQMGCGGAYRLEGEGDAAYLN
                     PLVYRGLSEEFVRFAGRLPLKGSAIEVAAGSGQPLVWDVQSSPAEPDMKQALKDEGIE
                     LVVSVPLMAKGRLIGAIQMGAPEIRAFAP"
BASE COUNT         1619 a         2379 c         2606 g         1438 t
ORIGIN      
        1 agcggcgcgg agcgcttgtg cgggcggggg gacttcctgg ctgtctccgg cgcgggggtg
       61 attcgcttcc aggctgcctt cgccgcgccg gaagagatcg cgcgactcgt cgagctgatg
      121 cttgggacgg aggggcacaa agtcatctgg gaacaactac aaccagcgtt ggagcctgtc
      181 tgaaaaggtc gctggagtaa ccactaagac accaagacac caagtgtctc aagagctttt
      241 ctttgtgcct tggtgtcttg gtggtgagca agaatgaaat gaagaacggc ggtatcgtcg
      301 cggttggatt tgtctcgctc gtgattgcag tggcaatcat cgtcggctcg cgcctgtcga
      361 gcgagacagt ggccgcgctg gcgggcgctg cttgcggcgc cgggctggct gcccctctag
      421 ggatcgccat cggaatggtc gtgggatcgt cacgcagccg cagcaccgcg acgcctgttc
      481 cgccgccgca ggtcgtcgtg attccaccgc agaaccctcc cccttcccat gcgcccttgt
      541 atccaccaac actggagcct ctcgtgccaa ggtcacgccc gttcacgatc attggcgaag
      601 acgactgaaa aaaacgtgcg acgcactttg gaagtgcgtc gcacgtggga ggagaccacc
      661 ggaggctgcg ttctacgctt tcagcttgta accgacgttg cgcacagtga cgagccggcg
      721 cggcctcccg gcgtcgactt cgatgatttg gcgcagccac cggatgtgca cgtcgagcgt
      781 gcgcgtatcg cccatgtagt cggtattcca cacggctttc atcaattggc ggcgcgtgat
      841 cacttcgttc ggctggcgca tgaagatctc gagcaactgc gactgcttgg gggtcagttg
      901 gtgctctttc ttacccagcc gcacgcagcg ggtggccacg ttgagcatga catcgtccag
      961 cgtcaatacg tcggcctcgc cgctggggag catgcgcgcg acacggttca gcagcttgcg
     1021 cggggtgaag ggcagcctca gaacgaaatc cgctccgctc gtcgcatcga cttcgacgtt
     1081 cgagcgcacc agcagcagaa tagcgatacc gtcgacgcgc gcccggatgg cgcgacatag
     1141 gcgcgggccg tcgatgcgcg aggaggtcga gtcgagaacg atcaaggccg gcgcctcgtc
     1201 tctcgcgcac gccagcgcct gcttgacggt cgaggcgcga atcacgtcat actttcgccg
     1261 ctcgagatac ggcgcatacg aggacttcga gcgagcgctc tcgatcaaga gaatagtcgg
     1321 cataccgcgc gcctgaggtg tgtgactgat cccgtccagc gggtacgacg gtgcggacgc
     1381 agggagcaaa aagccgcggc cgggatgacc ggcgcacggc agggttgaac cgagcgatct
     1441 acttcaggat ctgtgtcttc agaaacttgt cgagatagat gatggcgatg gcgccgaaca
     1501 tggtggacgc cagaatgaaa ttcagcgccc cgaacttctg cagcaggagc ggatcgctgc
     1561 tggagatctg gaggaagtaa acgatgccaa atgtaaccgc cgctccgatc gccaggccga
     1621 cgatgatcac aaccgcacgc ttgagcgcca tgcacacccc cgatgctgaa gtactgtcat
     1681 cttaacccaa cctggacgaa gcgtcaagta ggccgtcggt tctatcttac gcggcatgcc
     1741 gccgcagcac gacacgcggc agcgcgcgca actcttccga acccatcgcc agcgccgtca
     1801 cgacgtacac gagcatcccg acgccggcgg cgatcgagaa tggaatgccg atctcgagcg
     1861 ccgagcgaag cgtcaagcta acagagactc catctggtag cggccacttc gcgaacacaa
     1921 cctgtgaaac agcgattgcc aggcccataa caaccgacgc cgccacaaag cgtgccagcg
     1981 atgccgcgat gcggcgcccc tcgatgccgc gcaagcggcg gccggcgatg agcagcagag
     2041 caaggacttc gagactgacg ccgatcgagt tggccagcgc cagtccggcg tgtccgaaag
     2101 cctcgcgcag aatcaggctg gccgcaataa caataatcat ggcgccggcg gcgacatata
     2161 aaggcgtcca ggtgtcttgc tgcgcgtaga agatgcgggc gcacacttcg agcatggagt
     2221 gcgcgatcaa accgagcgcg taggcttgca gcgcccagac cactgcgtca gtcgcggatg
     2281 cctggaacag cccaccctcg aacaatacgc gcacgaatgg acgccccaga acgatcagcc
     2341 cgacggcggc cggcaccgtg agcgccagga tagtgcgcaa agtcgaagaa attgtcgcgc
     2401 ggagttccgc gattttgcct gaggccgcca gctcgctgag cgtcggaaac acggccgtcg
     2461 caactgccgt ggcgatgacc gtctcaggca actgcatcac gcgccaggca tagttgagca
     2521 cggtgacgct gcctgggctt aactgcgagg ccaggcttgt ggtcatgaca aatgtgagtt
     2581 ggacgactcc caatccggcg acgcgcggca cgagcagctt gaggactcgt cgaagaagaa
     2641 catcgttcag actgagaacc ggcgaccagc gcgcaccgta gcgcgccagg ccgggcactt
     2701 gaacgagtag atgcagcact gctccaacga ccacgccgat cgccggcccc cacacgccga
     2761 tcgagggcgc cagcaggatc acgcctgcca gaatacccag gttgtacagg atcggcgcga
     2821 gcgcgggcag aaggaagtgc tgatgtgcct gcaaggtgct catcacgacg gcgctgaccg
     2881 agaagatcgc cgtcgagatc agaatgacgc gcatgagatt ggctgtgagg acgacctgtt
     2941 cgggcgtgaa acattgctcg ttcgccgcgc tcccttgatg acatccgtat aggacgtcgg
     3001 cgatccacgg agcgaaaaga gccaagccaa tggcaaagca caaggtcacg agaaaggcga
     3061 gactcaccac ggcgctggtc agtcgccatg aagcggctcg atcgcgcgcg atcaggccag
     3121 agagcaccgg tatgaacgcc gacgccagcg cgccgccggc gacaagcgtg aagagcagat
     3181 cgggcaggct gaaggcggcg tagtaggcat ccagctcgcg gcccgggcca atgatgcggg
     3241 cgaggatgag gtcgtccagt atgcccgtga ctttgctggc gagaaagcct gcgccgacga
     3301 gcagcgccgc gacggcgata cgccggttgc gagtgagagt ggcttcggca gactgggaat
     3361 ccgtcatcat ttggcccggt cgcattattg gcccaacacc gcaagtgcct cggcagcagc
     3421 cgtgaagttc gggttgtaat ggagcgcctg cctgaactgc tcgcgggcgg cgtcaggctc
     3481 ccctcgcgcc tggcgcacga ggccgcggta gtaccatagc tcttcgacgt acggcgtcgt
     3541 ggcgagcgtc gcatcggcca gcgcaagcac gtcgtcaaag cgccccgtcc ggtaatacgc
     3601 ctcgaatatg ccgaactgat accaggtcat gcgccagggt agccccagac tgcgggcctg
     3661 gtcgtaggcg gcggcggcgg attcgtaatc gcccagtgcg acgaagttgc tcccgacgtt
     3721 gaaccaggcg aatggattgg aaggattggt cgtcgcctcg gcttgcgccg tggcaagcgc
     3781 gccggtgagg ttctcggttg gatcggcatc cgcgccgagt atctgcatga ccgtgccggc
     3841 ctgatccggc gtgtggacga tgatgaaggt gcgattgaag tggctccaat accagtcaag
     3901 tgtatcgtat gtcatggatt gattcgggcc ggtgtacgag tccatgagga cgaacgcgtt
     3961 tttcgaatca tcgtaaccca cgaccagccc ataatggcct tcccatcccg attgcgggtc
     4021 aggctcgaag cctttttcga tcaacacagg aatgcccgcg cgcaggaatt ccttgacgag
     4081 cggaatcgat ccgtcgacgc gcatcacggc ttcgaaaccc agcgaccggg caaaggccgt
     4141 catttcgtgc ggcgagacat tcttgtcctc cggatcgggt ttgaggaatt tcgcggcgtc
     4201 ggcctggccg ccgccccagc cgtagaagct gagattcatc gacagggtag ccggcccgca
     4261 gttgttccac gtctgataga tatgccgaaa accggtcaac agcacgtcgg tcgattcgag
     4321 cgacgccggc gtggagcgcg cggcctgcct cggtccgggc gggatgacgg gcgtgagcgt
     4381 gagtcgtgcc gccggttggg tctgcgcaag gatgtcagtc tgacccggcg ggtcggtcgg
     4441 ttcaggtgtt tgggaagtca acgtcggagt cggagtgcgt gacgacgccg gcgtgggcga
     4501 aggagctggc gtcttcgttt gacgcggcgc aagcagttcc gatgcatcca ttgtaatctg
     4561 cgccgccggc gtcgggacga cgtcgggata gtcgggcaaa atcagcgagc gcgccgccgg
     4621 gcggaggaaa gacggcagcc ggttgatgag gctagcctgg tagcgtggct gcagcgaacg
     4681 cacggcgaac acgccggcca tcaacatcac ggcgccgatg gctgctgcaa agaaagccca
     4741 ccgacctgcg tatcgcgtct tcatctcgag cctcgcatga tttgccgatt atacaccgcc
     4801 gcgaaagtaa agcgcccgat cccctggtca acgattgcac gacgattgcc caccgtgcgg
     4861 gattctgtgt ataatcgcag cattcccgct ggaggacgcc tatgatgcgc caagaagtcg
     4921 tgacctggac ggatctggaa atgctcgtcg atacgctgat accgcaactt tacggcgtct
     4981 tcgacgcact gctgctgatt acgcgcggcg gcatcgtgcc gggcgggatg atcgccgagg
     5041 cgctcgacat taagtacgtg ttgaccgctg cggtgcggtt ccccgaagtc gatcagaaac
     5101 tgctggcctg gccgaccttc ctgcaattcc ccgaggatga cctgctgcgc aaccggcgtg
     5161 tgctcgtcgt ggacgacgtg tggggcagcg gacggacgat caactcggtg cgcggacgca
     5221 tcgaggcggc cggcggcaag cccgaagtgg ccgtgctgca ctacaagccg ggcgaatcgc
     5281 tgttcacgac gaaacccgaa tactacgccg ccgtgacgga cgcgttcatc atctatccat
     5341 gggagaaggg gcgcggcacg caagggattt tccgaaggtt ggagccgagc ctgatctgag
     5401 cctgaatttc gcacccagca ggtaccgaag ttgggacggc agttgcactg ccgtcgccgc
     5461 agttggattg cggcgaaagc acgagccgca gtttatgttt acccccaatc tgaaatgcgc
     5521 ccagaggcaa tgcagttgac tttgttcaga aaccgccgta tactggggtc ggtctcaagc
     5581 agattgaagt gaatcttttc tgacgtagtc gtgagaagaa taaaccaagg cgggggcgcc
     5641 ttggtgggga cgtttgatat agtgcccgcc caccaagttg gtggacgggc ttttgcgtca
     5701 aaatgagaca tcgccacgat ggcggaggcg cgactatgca gagcagagaa gaacggctag
     5761 atctggtcct gcaaggcaca aatgatggga tttgggattg ggacatgcgc acgggcgagg
     5821 tctacttctc cccgcgctgg aagagcatgc tgggctatgc ggacgacgag gttgccaatc
     5881 gaaaagacga atggaatcgc cttctccacc cgaatgacct tgaacgggcg ttggctgcgg
     5941 tgcaggacta tctggacggc cggacaacca cctaccagct tgagcaccgc cttcgccaca
     6001 aagacggcac gtatcactgg gttctggccc ggggcattgc gcttcgcgac gaggcgggcc
     6061 gggcctaccg catggtcggt tcgcataccg atatcaccga tcgcaagcac gcggaacagg
     6121 cgctactcga gagcgagacg aacctgcgct cgttgatgga gaacgccagg gactttgccg
     6181 tgtatcgcat cgccgtcgat ccctcgaatc cctatctggc tcgcgtcgtc gtcgccagcc
     6241 cttcgctgaa agaggtcttc ggcatccccg acctccatga cttcacgacc tggttcacac
     6301 ccatccatcc cgaggattta tcccgaatcg tcgaagcgaa tcgacgctcg cttgagatgg
     6361 gcgtgccgtt cgatcagcaa ctgcgcttct ttaacgccga acgcgggagc tgggtctggg
     6421 cgcacgtgcg atcttcgccc gtgtttgata ccgacggtag actgacccat ttcaacggcc
     6481 tggtggttga catctccgag caaaagcggg ttgaagatga gatgcgtcgc caggacgaac
     6541 tgctgcagaa acgcctcgct ttcgagaatc tcatcagccg catctcgacc gaattcatca
     6601 acctcggtcc caacgagatc gacctgggca ttcggcatgc gctgcaggcg attggcgaat
     6661 tcgctggagc cgatcgcagc tacatctttc tcgtttccgg cgatggcacc cggatggcaa
     6721 acgcacacaa atggtgcgcc gaaagcatcg agccggccat cggacgcgta gccgaggtgt
     6781 cggccgagtc ctttccgtgg ttcatcgaga gaatgaatcg tctggagatc gttcagattc
     6841 cgcgcgtcgc agatctcccg cccgaggccg gcgccgagaa gcaggaattc cagtcgcaat
     6901 ccacccaatc gctcctcgct gtgccgatgg tctatcgcgg ctcgttggtg ggattcgtgg
     6961 ggttcgactc ggtgcgaagc gaaaaggcgt gggacgaaga tagcatcgcg ctgcttcgca
     7021 tcgtcggcga aatcttcgtc aacgcgctgg agcacaaacg cgctcaggcc attcaggcgg
     7081 gacagcgcca gtttctggag ttgctggcga ccggtggcga tttctcaggc acgcttcaca
     7141 cgctcgtgcg tctgatcgaa gagcagtggc cgggtatgct gggactcgtt ctgctcctgg
     7201 atgtggatgg ccgtcacttg catatcgggg cgtccatcag tttgcccgaa gactacgtcc
     7261 aatcgatcga ggggctggag atcgggccga tggtcggctc gtgtggaacg gcgtgctacc
     7321 ggcgcgagcg cgtgatcgtc gaagacatcc tgaccgatcc gcgctgggac ggcctgcgcg
     7381 atctggcggt gaagtatggg ctgcgcgcgt gctggtcgga gccggtcttc tcggccggtg
     7441 gacaggtcgt gggcacgttc gccatgtact atcgccatcc ccgcgcgccg actgccgccg
     7501 aactgcgcgc gatcgagatg gccgcgcacc tggtcgggat cgctatcgag cacaagcgtg
     7561 cacagaaagc gcttcaggcg gcgtatcaaa cgctggagca gcgcatcgag gagcgcacga
     7621 gagaactggc ggcgctgaac gcgatcgccg ccgtcgtcag ccgctcgctc gacttgaagg
     7681 aagttttgag caacgcactc gacaagacca tggaagtcat ccagatggga tgcggcggag
     7741 cgtatcgcct ggaaggagag ggagacgcag cgtatctgaa tcctttggtg taccgtggcc
     7801 tatccgaaga gttcgtccgc tttgcggggc ggctgccact caagggcagc gcgatagaag
     7861 tcgccgccgg cagcgggcaa ccgctcgtgt gggatgtgca gagttcgccg gccgagcctg
     7921 acatgaagca ggcgttgaag gacgaaggca tcgagctcgt cgtatccgtg ccgctgatgg
     7981 cgaaggggag gctgatcggc gccatccaga tgggcgcgcc ggagattcgt gccttcgcgc
     8041 ct
//
LOCUS       DBNHXB010000209        10136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2395986, whole genome shotgun sequence.
ACCESSION   DBNHXB010000209 DBNHXB010000000
VERSION     DBNHXB010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10136
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2395986"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="AC162G_08545"
     CDS             complement(<1..828)
                     /locus_tag="AC162G_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075901.1"
                     /translation="MKSIGPLVLLILLLFSRPAQANAIELSYELAVYYALPGDIPFDQ
                     EVFNRIEEASLEIQAWYQVATGGATWELAYPEIVRVYHGQNTRQFYKDTGNWWGSLLP
                     EMDSAGLPVWEPGIVVVIWAHGAEWWAGGAQGCGIDCGVVLLGVELFPEFNNPLWTGQ
                     DCPDPDGQGVEAWPCTPLGAYAHELGHTLGLIHPLDDPVTAPYAWHSIMQAHWNYPNE
                     APPEDRPWGFLRSERQHMQSNPFMKTGVPLIQVHQDADIAVNLPLSGTLPLADFMVEV
                     "
     gene            1208..2413
                     /locus_tag="AC162G_08550"
     CDS             1208..2413
                     /locus_tag="AC162G_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR5075902.1"
                     /translation="MSEMRRPIYLDHAATTPVDPRVIEAMQPYWTGAFGNTASIHGVG
                     RAALKGLEQARQTVADVLGCHPMEIVFTGSGTESDNLALRGVAWAARRSGRGEHIITT
                     PIEHHAVGVTAEQLRDLHEFDVTFVDVDAHGRVDPDAVGRAIRRDTCLVSVMYANNEV
                     GTIQPLRDIGALTRSKGITFHTDAVQAGGALDLNADRLNVDLLALSAHKFYGPKGIGV
                     LYVRRETELEPALTGGGHERGRRPGTVNVAGAVGLATALKLAQDNRESENARVAALRD
                     RLIAGVLERVPDARLTGHPTERLPNSASFAIKGVEGESILLALDLEGICVSTGSACTT
                     GEAEPSFVLTAMGLPREWAVGSLRMTLGHPTQPEDIDTVLEVLPRVVERLREHNLSPF
                     ARGAATFGR"
     gene            2474..3547
                     /gene="mnmA"
                     /locus_tag="AC162G_08555"
     CDS             2474..3547
                     /gene="mnmA"
                     /locus_tag="AC162G_08555"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR5075903.1"
                     /translation="MTTDRKSTRVVVAMSGGVDSSVAAALLVEQGYDVAGIMLKLWSE
                     DGGSRVNRCCAPADVALARSVADQLGIPFYLINVADSFKSIVVDFFVEEYAAGRTPNP
                     CIECNRRIRFELLLNKALGLSAQYLATGHYARVRNSEGVYQLLRAVDRRKDQSYVLSV
                     LGQVQLAHVMFPLGEMTKPEARAHAARLHLPVAEKPESQDLCFLGNEDYREFLLRHAP
                     ESIRTGEIRDTAGNVLGQHQGLPFYTIGQRKGLGIAAAEALYVIAKDAADNSLVVGPL
                     AELGRRECVARGMHYVSGVTPAEPFRAEVKIRYTARETPATVHPAGDTARVEFDELQR
                     DVTPGQGLVLFDGEVVLGQGIIT"
     gene            3548..3796
                     /locus_tag="AC162G_08560"
     CDS             3548..3796
                     /locus_tag="AC162G_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075904.1"
                     /translation="MPPALLFSALLSTAYAALFHLWRGGGVRRLGLFLVAAWIGFALG
                     QAAGSLLGWNVAMLGEVHLLEATIGSAMALVIVSRPTV"
     gene            3821..4267
                     /locus_tag="AC162G_08565"
     CDS             3821..4267
                     /locus_tag="AC162G_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075905.1"
                     /translation="MQLETNPDLPPPAPKPSGSRVSRRVVLAASLAVLVVILVVGAFV
                     LMVRAGTTDVFRDISIILLALVMMVIGLLLLVLIYQLALLTKMLNDEIKPLLQNLQET
                     ASTARGTTVFLSEHVVHPVIGAAGTMAGFARIVGLLSELGRVWRRR"
     gene            4315..4554
                     /locus_tag="AC162G_08570"
     CDS             4315..4554
                     /locus_tag="AC162G_08570"
                     /inference="COORDINATES: protein motif:HMM:NF024143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIR5075906.1"
                     /translation="MNSNTSSIVGGFLAGIVIGGVIGAAVALLLAPQTGQETRRQLES
                     KAEELRGRAELLAEDARKRVEAASEDLRKRVEKPA"
     gene            4745..5089
                     /locus_tag="AC162G_08575"
     CDS             4745..5089
                     /locus_tag="AC162G_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIR5075907.1"
                     /translation="MSDDGNEFGAFLAGFVVGGLIGAVVALLMAPQSGEAMRRQIRER
                     AYELRDRAEDIAEDTRRRIETTGADVRRQAEEQRVRLEQAVAEGKKAAERKRAEMMGS
                     APDSGDSASSST"
     gene            complement(5213..6865)
                     /locus_tag="AC162G_08580"
     CDS             complement(5213..6865)
                     /locus_tag="AC162G_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR5075908.1"
                     /translation="MKLVTVEQMKAMEVASDAAGHSYDAMMDRAGAAVAQVVQQRGAV
                     KGKRILVLVGSGNNGGDGLVAAGHLREAGAEVACYLLKPRDDARVTAAREAGAFIGAL
                     PDDRGLRVLRRFSLGADVVIDALFGTGARLPLPHEAHMLLNTVKGDIEARRNKDAALT
                     APASPAAPAGPLIVAVDGPSGMDFDSGALDSSALPADVSVTFAYPKIGHTRFPAAEAC
                     GELIVADIGVDPALASSVTRELADPHMIRRLLPARPASAHKGTFGKVMIVAGSIHYSG
                     APALAAAAAYRCGAGLVTVAAPRPVLPIVASRLTEATFQPLPDSMGIVSADAVPILSK
                     ALEGYRAVLIGPGLTHEKEAAAFLERLLGGEPPSNRRMGFSTESDSASPPARVSLPPL
                     VLDADGLNVVSEKEEWWKLLPESSVLTPHPGEMVRLTRLERDEVEADRIGVASRFSQT
                     WGHVVVLKGAFTVVTAPDGRIVVMPFANPALATAGSGDVLAGAIAGLRAQGLNAFDAA
                     LCGAYLHGLSGHNARTEIGKAGVVAGDLLERLPAAVRQLTGV"
     gene            complement(6862..7890)
                     /locus_tag="AC162G_08585"
     CDS             complement(6862..7890)
                     /locus_tag="AC162G_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5075909.1"
                     /translation="MILVTGATGFLGRNLIPLLVSRGHHVRALVRRSSAWQYLADLGV
                     ELAWGDVRDAGTVQDAMPGCRVVVHAAGLFRFWGNREDFSATNVGGTHHALEAARQAR
                     VERFVHISTVAVVGRPKPETTIDEGYPCEPADDYMRSKLEGEGLVRRYWQEHRLPAII
                     LRPGAYYGPWGHYAFNRLFFEDPFIRGLPIRVHGGRRFTFPVYVPDLASAIETTLTHG
                     RPGETYNVSGLSLTHREINATISRITGVQPRWIDVPAPAILLFARLWTAFSRLTGREP
                     YYPINLAHYVFHDWVVDSHKARRELMFEPTPFEEGARATIEWYRSIGMLTHSGPRLQA
                     SPLTREEA"
     gene            complement(7887..9806)
                     /locus_tag="AC162G_08590"
     CDS             complement(7887..9806)
                     /locus_tag="AC162G_08590"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR5075910.1"
                     /translation="MLNPFGRLLSVDLSRSTITEQQIDPDLTRDYVGGSGLAARLLWD
                     RLAPDLDPLGPDNPLLFITGPLTGTAGPTNGRFSVCARSPATGLWGEANCGGFWGPEL
                     RFAGYDAVLITGKAASPVYLWIRDGVAELRGASHLWGQADTYQTQERIREEAGDRLAR
                     VACVGVAGERLIPYALILCDHGRVAGRAGMGAVMGSKNLKAIAVRGKGKLPIAQPQFF
                     NTFRSTLNRALREENITLSLRAAGSASAAEYLQMLGEMPMRYFTAGQFEGQENVSGSV
                     IADTILTGVTTCHACVIACGRLVTITEGPYTREKSKGPEYETMTGFGSILGSGDMNAA
                     THLGQLCDVYGLDTISASNVIGLAYLLFDRGMLMERDTGGLKLAWGDPRPAEALLHLI
                     AQREGIGEVMSRGAKALGAHFGVEELAAHVNNLEMPYHDPRALSGMGIVYATSPRGAC
                     HNQSDFFVAEIGADHEEIGIPLLTPPTPDAGKAALVARHQDYRSLSNSLIVCIFARLP
                     VTSQLILYNAAVGLDRDIGEFMQAGARIWNLKRVINHRFGLTRANDKLPKLLREPLPD
                     GPNAGRVPDFDLLMREYYAARGWDPVTGRPTRATLEALDLGFAADTLDRAGARPAPTT
                     LQAPPSNLHPRTSSP"
     gene            complement(9957..>10136)
                     /locus_tag="AC162G_08595"
     CDS             complement(9957..>10136)
                     /locus_tag="AC162G_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HIR5075911.1"
                     /translation="INAPLANWKRVITKQIGPIPAMVSGQLKVRGNLPYILRHVSAAQ
                     HLVECATRVDTEFPA"
BASE COUNT         1781 a         3171 c         3411 g         1773 t
ORIGIN      
        1 aacttccacc atgaagtcgg ctaagggcag tgtaccgctg agtggtagat ttacggcaat
       61 gtccgcgtct tggtgaactt ggataagtgg gacgccggtt ttcataaatg ggttactctg
      121 catatgctgt ctttcacttc ttagaaaccc ccagggacgg tcttctggag gagcttcatt
      181 gggataattc cagtgcgcct gcataatact gtgccatgca taaggggccg tcaccggatc
      241 atccaatggg tgtatcaatc ccagggtatg gccgagttca tgggcgtagg ctcccagagg
      301 cgtacagggc caggcttcca cgccttgacc atctggatcg ggacagtcct gtcccgtcca
      361 gagaggattg ttgaactcgg gaaacaattc cacgccgagc agaacgaccc cacaatcgat
      421 accacaccct tgcgctcctc cagcccacca ttctgcgcca tgtgcccata tgaccaccac
      481 gattcccggc tcccagactg ggagtccagc actatccatc tccggtagta aagagcccca
      541 ccagtttcca gtgtccttgt agaactgtcg agtgttctgt ccatgataga ctcgaacaat
      601 ctccggatag gctaactccc atgtcgctcc gccggtggcc acttgatacc atgcttgtat
      661 ttccaaagaa gcttcttcga tcctgttgaa cacttcttga tcaaaaggaa tatccccggg
      721 gagggcataa tacaccgcca gctcataact caattcgata gcattcgctt gtgccgggcg
      781 tgaaaacagt aataagatga gcaaaacaag tgggccaata ctcttcatct tatgctcctg
      841 tcttggccat gcgcgcgcac caacggtgga gttcagcctg ttactttata ctcgacaaag
      901 ggaggccaat ggggtaagct acctgcagag cggggtgacg acctgtcacc atctgcccgc
      961 ttcgcgccat cggcggaccg tcccccaggc taccgagctg gaccgctcaa gcctcacggg
     1021 tgtgagccaa aacggggaca cagtcacccg cgtccgccgg cgggccttcg gagccacgac
     1081 acgggaggtc agtatgacag tgtacatcgg aatcgactgg agtcaagccc aacatgacgt
     1141 ggatcagctc ggtcgttgac gcgcccattc ccgcgtgcta caatccttca cgttcagggg
     1201 gataaagatg tctgagatga gacgcccgat ctacctcgat cacgcggcca cgacccccgt
     1261 cgatccgcgc gtcatcgaag cgatgcagcc atactggacc ggcgcgttcg gcaataccgc
     1321 gtcgatccac ggggtggggc gcgcggcgct gaagggactg gagcaggcgc ggcagaccgt
     1381 cgccgacgtg ctgggctgcc atccgatgga aatcgtgttc acgggcagcg gcactgagag
     1441 cgacaacctg gccttgcgcg gcgtcgcctg ggcggcgcgg cggagcgggc gcggggaaca
     1501 catcatcacg acgccgatcg agcatcacgc cgtcggcgtg actgccgagc agctccgtga
     1561 cctgcacgag ttcgacgtga ccttcgtcga cgtggacgcg catggccggg tcgatccgga
     1621 cgccgtcggc cgcgcgatcc gccgcgatac gtgcctggtc agcgtcatgt acgccaacaa
     1681 cgaagtcggc acgatccagc cgctgcgcga catcggcgcg cttacccgca gcaagggaat
     1741 cacctttcac accgacgccg tgcaggcggg cggagcgctc gatctgaacg ccgatcggct
     1801 gaacgtcgac ctgctcgcct tgtcggcgca caagttttac gggccgaagg ggatcggcgt
     1861 gctgtacgtc cgtcgcgaaa cggaactcga gccggcgctg accggcggag gccacgagcg
     1921 cggccggcgg ccagggacgg tcaacgtcgc cggcgcggtc ggattggcga cggcgctgaa
     1981 gctagcgcag gataaccgcg aaagcgagaa tgcgcgtgtc gccgcgctgc gcgatcgact
     2041 gatcgccggc gtgctggagc gcgtgcccga cgcgcggctc accggccacc cgaccgagcg
     2101 cctgcccaac agcgcctcgt ttgccatcaa aggcgtcgag ggcgagtcga tcctgctggc
     2161 gctcgacctg gagggcattt gtgtctcgac cggctcagcc tgtacgacgg gtgaagccga
     2221 gccatcgttc gtgcttacgg ccatggggct gccgcgcgag tgggccgtcg gctcgctgcg
     2281 catgacgctg ggacacccga cgcagcctga agacatcgat acagttttgg aggtgctgcc
     2341 gagggtggtt gagcggttga gagagcataa tctatcgccc ttcgcgcgag gtgcggcgac
     2401 ttttggaaga tgatacgccg gatggcgagg atgtgctgcc gggcttcgtg gcgccggtga
     2461 aagagttgtc tgaatgacta ccgatcggaa gagcacgcgc gtcgtcgtcg ccatgagcgg
     2521 cggcgtggat agctcggtcg cggcagctct gctcgtcgag caaggctacg acgtggcggg
     2581 catcatgctc aagctgtgga gcgaggacgg cggatcgcgc gtgaatcgct gctgcgcgcc
     2641 ggccgacgtc gccctcgcgc gctccgtcgc cgaccagctt ggcatcccgt tttacttgat
     2701 caacgtcgcc gactcgttca aatccattgt cgtcgatttc ttcgtcgaag agtacgcagc
     2761 cggccgcacg ccgaacccgt gcatcgaatg caatcgccgc atccgatttg agctcctcct
     2821 caacaaggcg ctggggctga gcgcgcagta cctggcgacg ggacactacg cgcgtgtgcg
     2881 gaattccgaa ggcgtctacc agttgctgcg agcggtcgat cgcaggaagg atcaatccta
     2941 cgtgttgagc gtgctcggac aggtgcaact ggcgcacgtg atgtttccgc tcggtgaaat
     3001 gacgaagccg gaggcgcgcg cgcacgccgc ccggcttcac ttgcctgtgg ccgagaagcc
     3061 ggagagtcag gacctgtgct ttttgggcaa cgaagactat cgcgagtttc tgctgcggca
     3121 cgcccccgaa tcgattcgga cgggcgaaat ccgcgatacg gcggggaacg tgctcggcca
     3181 gcaccaggga ctgccgttct acacgatcgg gcagcgcaag gggctgggca ttgcggcggc
     3241 ggaagcgctg tatgtgattg ccaaagacgc cgccgacaac tcgctcgtcg tcggcccgct
     3301 cgccgagttg ggccggcgcg agtgtgtggc acgcggcatg cactacgtca gcggcgtgac
     3361 gccggccgag ccgtttcgcg ccgaggtgaa gatccgctac acggcgcgcg aaacgccggc
     3421 gacagtgcac ccggcaggcg atacggcacg cgtcgaattt gacgagctgc agcgtgacgt
     3481 cacgcccggc cagggtctcg tgctgttcga cggcgaggtc gtcttgggac aagggatcat
     3541 cacctgaatg ccgcccgcct tgctcttctc tgccttgcta tccacggcgt acgccgcgct
     3601 ttttcacctg tggcgcggcg gaggtgtgcg ccggctggga ctgttcctcg tcgccgcatg
     3661 gatcggcttt gcgctaggcc aggcagccgg ctcgctgctg ggctggaacg tcgccatgct
     3721 cggcgaggtg catcttctgg aagcgacgat cggaagcgct atggctttgg tgattgtcag
     3781 ccgcccgacc gtatgatata atgcccgtcg aggctcctgc atgcagctcg aaaccaaccc
     3841 cgacttgccg ccgccggccc cgaagccgtc cggatcccgt gtcagccggc gcgtcgtgct
     3901 ggccgcgtca ctcgcggtgc tggtcgtcat cctcgtcgtc ggcgcgttcg tactgatggt
     3961 gcgggctggc acgacggatg tctttcgcga catttcgatc attctcctcg ccctcgtcat
     4021 gatggtcatc ggtttgctgc tgctggtgct catttatcag ctcgcgctgc tgaccaagat
     4081 gctgaacgac gaaatcaagc cgctgctcca gaacttgcag gagacggcga gcacggcgcg
     4141 tggcacgacg gtattcctga gtgaacatgt tgttcatccg gtgatcggcg cggcgggcac
     4201 gatggccggt ttcgcccgga tagtcggctt gctctccgag ctggggcgcg tgtggcgccg
     4261 ccgatagccg gcgtcgacgt ggtcgaaagt gcaggcattc gataaggagg gaacatgaac
     4321 agcaacactt caagcattgt tggggggttt ctggcgggaa tcgtcattgg cggtgtgatc
     4381 ggcgcggcgg tggccctgct cctcgcgccg cagaccggcc aagagacgcg gcgccagctc
     4441 gagtcgaagg ccgaagagct gcgtggccgg gcggaactgc tcgccgaaga cgcgcgcaag
     4501 cgcgtcgaag cagcatcgga ggatttgcga aagcgcgtgg agaagccggc gtaaactata
     4561 ctacctgcac gtcagatttc cgaacctcgt aggggcaacc ctggcggtcg cccctgggca
     4621 ggttacatcc atacctgcca tgaagaaggg ggctgatgaa agaaggcggc taagaaagcc
     4681 gccctacgtc gagtcttcaa gtgcaggcgg tatagaagcc tatccgagaa cggaggaccc
     4741 ccacatgagc gacgatggaa acgaatttgg agcttttctg gccggcttcg tggtcggcgg
     4801 gttgatcggc gcggtggtcg ccctgctgat ggcgccgcag tccggcgagg cgatgcgccg
     4861 gcagatacgc gaaagagcgt acgagctgcg cgatcgcgcg gaagacatcg ccgaagacac
     4921 gcgccggcgc atcgagacga cgggtgccga tgtgcgccgc caggccgagg agcagcgcgt
     4981 gcgcctggag caagccgtcg cggagggcaa gaaagcagcc gagcgcaagc gcgccgagat
     5041 gatgggttct gcgcccgact ccggcgattc ggcttcatcc tccacctgag cgcagtgggc
     5101 aacttcaatt cgaaaaggcc ccgatctctg tcgagatcgg ggcctcttgc gtgtgcgagc
     5161 cagcccccct ggccctcccc ccgtcagaac ggggggaggg ccaggggggc ggttagacac
     5221 cggtcagttg cctgacggcg gccggcaagc gctcgagcag gtcacctgcg acgacgccgg
     5281 cttttccgat ttcggtgcgg gcgttgtggc ccgagagtcc gtggaggtac gcgccgcaca
     5341 aggctgcgtc gaaggcattc aatccctgcg cgcgaaggcc ggcgatcgcg ccagcgagca
     5401 cgtcgccgct ccccgccgtg gcgagcgcag gattggcaaa cggcatcacg acgatgcgcc
     5461 catccggcgc cgtgacgacg gtaaaagcgc ctttcaacac gaccacgtgc ccccaggtct
     5521 gcgaaaatcg cgacgccacg ccgatgcggt ctgcctcaac ttcgtcgcgc tccaacctgg
     5581 tgagacgcac catctcgccc ggatgcggcg tgagcacgga cgactccggc aggagcttcc
     5641 accactcttc tttctcagac acgacgttca gcccatccgc atcgagcacg agcggcggca
     5701 gagagacgcg ggccggcggc gaggcggagt cagattccgt cgagaagccc atgcgcctgt
     5761 tggaaggcgg ctctcctcca agcagccgct cgaggaaagc ggctgcctcc ttctcgtgcg
     5821 tgagacccgg gccgatcaga acggcgcggt agccttcgag tgctttgctg agaatcggga
     5881 cggcgtcggc ggagacgata cccatcgaat cgggcagggg ttgaaacgtg gcttcggtca
     5941 atctggacgc gacgatgggc aggacaggcc gtggcgcggc gacggtcacc agccccgcgc
     6001 cgcaccgata cgcggccgca gccgctaggg cgggcgcgcc tgaatagtga atcgagccgg
     6061 cgacgatcat gaccttgccg aacgtgcctt tgtgcgcgct ggcgggccgc gccggcagca
     6121 gccgccggat catgtgggga tcggccagtt cgcgtgtcac actggaggcg agcgccggat
     6181 cgacgccgat gtcggccacg atcagctcgc cacacgcctc ggctgccggg aaccgcgtgt
     6241 gcccgatctt tggatacgcg aacgtcacgc tgacgtcggc aggcagcgcg ctactgtcga
     6301 gcgcgccact gtcaaaatcc ataccgctcg ggccgtctac tgcgacgatc agcgggccgg
     6361 ccggcgcggc aggcgatgcc ggcgccgtca aagcggcatc cttgtttcgc cgcgcttcga
     6421 tatcgccctt gaccgtgttg agcaacatgt gcgcttcgtg cggtaaggga aggcgcgcac
     6481 cggttccaaa aagcgcatcg atgacgacgt ccgcgcccag gctgaatcgg cgcagcacgc
     6541 gcagcccgcg atcgtcgggc agcgcgccga tgaaggcgcc cgcttctctc gccgcggtga
     6601 cccgtgcgtc gtcgcgcggc ttgagcaggt agcacgcgac ttccgcgccg gcttcgcgca
     6661 gatgtccggc cgcgaccagc ccgtcgccgc cattgttgcc cgacccgacg agcaccagga
     6721 tgcgcttgcc tttgactgcg ccgcgctgct ggacgacctg cgcgacggca gctccggcgc
     6781 gatccatcat cgcgtcgtac gagtgccccg ctgcgtccga ggcgacctcc atcgctttca
     6841 tttgctcgac ggtgacgagc ttcatgcttc ctcccgagtc agcggcgagg cctgaaggcg
     6901 cggaccgctg tgcgtcagca tgccgatgct gcggtaccac tcgatcgtcg cgcgcgcgcc
     6961 ttcctcaaac ggggttggct cgaacatcag ctcgcgcctg gccttgtggc tgtccaccac
     7021 ccagtcgtgg aagacgtagt gcgccaggtt gatggggtag tacggctcgc ggccggtcag
     7081 gcgtgagaac gccgtccaca atcgcgcgaa gaggaggatc gccggcgcag gcacgtcaat
     7141 ccagcgcggc tgcacgccgg tgatgcggct gatcgtcgca ttgatttcgc ggtgcgtcag
     7201 gctcagcccg gacacgttgt aggtctcacc tgggcggccg tgcgtaagcg tcgtttcgat
     7261 cgcgctggcc aggtcgggca cgtagaccgg aaaggtgaag cgccgcccgc cgtggacccg
     7321 aatcggcaga cccctgataa aggggtcttc gaagaagagc cggttgaatg cgtaatgccc
     7381 ccagggaccg tagtacgcgc cgggccgaag gatgatggcg gggagtcgat gctcctgcca
     7441 atagcgccgc acgagccctt cgccttcgag cttgctgcgc atgtaatcgt cggccggctc
     7501 gcacggatat ccctcgtcga tggtcgtttc cggcttcggc cggccgacga cggcgacggt
     7561 cgagatgtgc acgaaccgct cgacgcgcgc ctggcgcgcg gcctccaggg cgtggtgtgt
     7621 gccacccacg ttcgtagcgg agaagtcctc gcggttaccc cagaagcgga acagtccggc
     7681 ggcgtgtacc actacgcggc agccgggcat ggcgtcctgc acggtgcccg catctcgcac
     7741 gtcgccccag gccagctcga cgcccagatc ggccaggtac tgccaggcgc tggaacgccg
     7801 cacgagggcg cgcacgtgat ggccgcgcga gacgagcaga gggatcaggt tgcgtccaag
     7861 gaagccggtc gcgccggtga cgaggatcat gggctggagg ttcgaggatg gagattggag
     7921 ggtggagctt ggagagtggt aggggcaggc cttgcgcctg cccggtcgag cgtgtcggcg
     7981 gcaaaaccaa gatcgagtgc ctcgagcgtg gcgcgggtcg ggcggccggt gacagggtcc
     8041 cagccgcgcg cggcgtagta ttcgcgcatc aggagatcga aatccgggac gcggccggcg
     8101 ttcggcccat cgggtagagg ctcgcgcagc aacttgggga gtttgtcgtt cgcgcgcgtc
     8161 agtccgaagc gatgattgat gacccgcttc aagttccaga tgcgcgcgcc ggcctgcatg
     8221 aactcgccga tgtccctgtc gagaccgacg gcggcgttgt acaggataag ctgcgaggtc
     8281 actggcaggc gtgcgaagat gcacacgatc aggctgttgc tgagcgaccg gtagtcctga
     8341 tgccgggcga cgagtgcggc cttgcccgcg tcgggcgtgg gcggggtgag aagcgggatg
     8401 ccgatttcct cgtgatccgc gccgatctcg gcgacaaaga agtcgctctg gttgtggcag
     8461 gcaccgcgcg gcgaggtggc gtagacgatg cccatgcccg acagcgcgcg cgggtcgtga
     8521 taaggcatct ccaggttgtt aacgtgcgcg gcgagttctt ccacgccaaa atgcgcgccg
     8581 agcgctttcg ctccccggct catcacttcg ccgatgccct cccgctgcgc gataagatgc
     8641 agcagcgcct ccgccgggcg cggatcgccc catgcaagct tcaggccgcc cgtgtcccgt
     8701 tccatgagca tgccgcgatc gaacagcagg taggccaggc cgatcacatt gctggcgctg
     8761 atcgtgtcaa gcccgtagac gtcgcacaac tggccgagat gggtggccgc gttcatgtcg
     8821 ccgctgccaa ggatcgagcc gaagcccgtc atcgtctcgt actcggggcc tttgcttttt
     8881 tcgcgcgtgt aggggccttc tgtgatcgtc accagccgcc cgcaggcgat cacacaggcg
     8941 tggcaggtgg tgacgccggt gaggatcgtg tcggcgatca cgctgccgct gacgttctcc
     9001 tggccctcga actgtccggc ggtgaagtag cgcatcggca tctcgccgag catctggaga
     9061 tactcggcag cgctggcgct gccggctgcg cgcaacgaaa gggtgatgtt ctcctcgcgc
     9121 aaggcccggt tgagcgtcga tcgaaatgtg ttgaaaaact gcggctgcgc gatgggtagt
     9181 ttacctttgc cccgcacggc gatggctttg aggttcttgc tgcccatgac cgcgcccatg
     9241 cctgcccggc cggcgacgcg cccgtggtcg cacaggatca gcgcatacgg aatcaggcgc
     9301 tcgccggcga cgccgacgca ggcaacgcgc gccagccggt cgccggcctc ctcgcggatg
     9361 cgctcctgcg tctggtaggt gtcggcctgg ccccacagat gcgacgcgcc gcgcagttcc
     9421 gccacgccgt cgcgaatcca caagtacacg ggtgaggcgg ctttgccggt gatcagcaca
     9481 gcgtcgtaac cggcgaagcg cagctccggc ccccagaagc caccgcagtt cgcttcgccc
     9541 cacaggccgg tagcgggtga gcgggcgcac acgctgaacc gcccgttcgt cgggccggcc
     9601 gttccggtga gcgggccggt gatgaacaac agcgggttgt ccgggccgag tggatcgaga
     9661 tcgggcgcga gtcgatccca cagcaggcgc gcggccagcc cgctgccgcc gacgtaatcg
     9721 cgcgtgaggt cgggatcgat ttgctgctcg gttatcgtgc tgcgggacag atcgacgctg
     9781 aggagacggc cgaagggatt gagcatggag cgtagagtga gagtttggag ttagagtctc
     9841 gagttacagt gagagctgga gttgaagtgg gctagcagct tgtcagagag agagcgggcc
     9901 gcgctcccac gcccggcggc atgggagtcg cctctgctag gctctccggc agcctgctag
     9961 gccggaaact cggtgtcgac gcgcgtggcg cactctacca gatgctgcgc cgcgctgacg
    10021 tgtcgcagaa tgtacggcag gttgccgcgg actttgagtt gaccgctcac catcgccggg
    10081 atcggcccta tctgcttggt gatgacgcgt ttccagttgg cgagcggcgc attgat
//
LOCUS       DBNHXB010000210        34121 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1053728, whole genome shotgun sequence.
ACCESSION   DBNHXB010000210 DBNHXB010000000
VERSION     DBNHXB010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 34121)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 34121)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34121
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1053728"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="AC162G_08600"
     CDS             <1..676
                     /locus_tag="AC162G_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5075912.1"
                     /translation="LEVFYGEFQALFGVSLEVTAGETIAIIGANGAGKTTLLNTIAGV
                     LTPRRGRVIYRGEVVSGSPSYRLCRRGISLVPEGRRIFPSLTVEENLEAGAYSGRRGP
                     WQRDAIYDLFPILRDRARAPGTELSGGEQQMLAIGRALMSNPDLILMDEISLGLAPVI
                     VRELYKVVRAVAEAGATVILVEQDVSRGLKAADRVYCLLEGKVSLAGRPAELSLAQVS
                     QAYFGV"
     gene            679..1551
                     /locus_tag="AC162G_08605"
     CDS             679..1551
                     /locus_tag="AC162G_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5075913.1"
                     /translation="MQWVNIVAQGVLLGGLYALYGTGLSLIFGVMRLVNLAHGDMIVA
                     AAYLALLAGQFLGLSPWLSLLLAAPALFIIGVLLQRGLLNATLGKGVLPPLIITFGLS
                     VITQNVLQLGFSADTQGLDAGPVEAASIEVVKGVTLGVLPLIVFATALVVLAGLHLFL
                     TRTKLGRAFRATSDDPTTAELVGINSRQVYGWAMGLSLAIGAVGGVLLGIRTTFAPTD
                     GPARLIFAFETVIIGGLGSLWGTLAGGIILGVAQNIGAQLSPAWFQLAGHLITLALLA
                     FRPTGLFARTRDTE"
     gene            1555..2655
                     /locus_tag="AC162G_08610"
     CDS             1555..2655
                     /locus_tag="AC162G_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5075914.1"
                     /translation="MGAPTPTPESSPATDHPKARPISPRGLALGSVLLLTALFSLPWW
                     GTPDQMRTLVEFITFLALAQMWNLLAGYGGMVSFGQQAWIGLGGYTLIVLADDLHVNM
                     FLAVIVGGVVAALVALPTAALAFRLRGGYFAVGTWVIAEVFRLLVASSTEWLKGGLGR
                     SLAAAGLMARPLREQYTYWLAIVIGAGAIALVYYLMRSRVGLGLTAIRDSESAAMSVG
                     VKITRIKLLVFVIGAFGTGVVGGLIYLNALRITPQASFSIQWSAFMAFMVVIGGLGTV
                     EGPIVGAVVFFVLRHYLSSLGPWSIILLGGTAVIIVLVAPDGLWGVLHRRFGLELFTI
                     RRRLPAAMAQGQLAMVEGKPASRDPALGEPPS"
     gene            2704..3486
                     /locus_tag="AC162G_08615"
     CDS             2704..3486
                     /locus_tag="AC162G_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR5075915.1"
                     /translation="MSIQIADVKQIVVEAGRLALLYYGKVSKSVKADLSVVSEADAAV
                     ENFLRLSLTALAPQYGYIGEETEESRPPAPGEEHAWVVDALDGSRAFVAQMPLWMPAV
                     CLMQGNQPVAGAAFNPVTNELFWAGEDGPAFCNDEPLHPTFSSALEPNTFIFGPTNHH
                     RTFTIDFPGRIYCLGAPIYQLCLVAKGAVAALFFDPAINLWDLALPSLLLERTGAVLV
                     YASGRPVFLSELMGRSKVPEPIFTGGAKVVEILRQRITYKHA"
     gene            3511..4038
                     /locus_tag="AC162G_08620"
     CDS             3511..4038
                     /locus_tag="AC162G_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR5075916.1"
                     /translation="MPDLRNMRLLPKPIIAAVNGLAAGAGAVIARAADLRVVADTARF
                     AFLFTRVGLTGADRGAAFLLPRVVGQGRAMDLLMLGGDVDAPTAERHGLANRVVLADQ
                     VLATAHDLAERLSAGPTYATGMTKLMVNNEWTMDLNAALEAEAQAQALMMMGEDHRAF
                     YEAFKEKRKPDFKGR"
     gene            complement(4056..5234)
                     /locus_tag="AC162G_08625"
     CDS             complement(4056..5234)
                     /locus_tag="AC162G_08625"
                     /inference="COORDINATES: protein motif:HMM:NF052764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR5075917.1"
                     /translation="MKAVNLMRIVVISMLIAACEESTAAQREIQPAPAASPGIAPDTV
                     SASGKVMPERWAVLSFQVSGAVKAVLAQPGDVVHTGDVLVRLDDVDAQMALAQAEAGL
                     ALAQAQLAQQTASPRSEQIAIAEQAVQSAEAELRAASAQLAQAQAGPPAAEIAAAQAA
                     LAAAARDLKVAQDTYDRVKDIGGTPEEQARAALEAARQAHRAAQERVNQLISGAAENE
                     LNALRAQVTAAQAKRDAARAQVELLQAGSSEEELSVAQATVAQAQVAVDAAQARLSRL
                     QIVAPFDGSVGAVSVRAGELAAPGQPVVTLGDLNRLHVETTDLNEIDIARVREGQTAL
                     VTFDALPGMALTGTVTRISPMSTPGQGTANFTVWIELAATDPALRWGMTAFVEISTEP
                     "
     gene            5630..7126
                     /locus_tag="AC162G_08630"
     CDS             5630..7126
                     /locus_tag="AC162G_08630"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5075918.1"
                     /translation="MSTTVPVDAPVVPLGAQVHDPVQAAIERANTRQDQDHIFFGITR
                     STCPECLALINAQILFRDGQVFMRKFCPEHGHFEALMAADADWWLHALSRTKPGTIPL
                     WHATQRKTQCPEDCGLCDDHQQHTCYPLIEITDHCDLHCPICFARNQHAWYLSPEQMT
                     FMLDRLAQAEGRAEIVCLTGGEPTVHPQFFELLDIAINHPVATKVAVNTHGLRLARDE
                     EFVRRIAETGAYIQLQFDGFKAETYRRLRGADLSKMKRLAFDHLAKYNVKTLLITTIA
                     QDLNEDAVGETIRLVLENDFIHSLTLQPLIYCGDALSNHLPHDMQRHITLTTILNLLE
                     AQMDGLMTKEDFVSIPCSHPSCSAVGYFLKAGPNDWVSLARITQADKMLNYLQNRLLF
                     NSEQWMRDLQSNLETLWSASAVMESEKVGRALSCCAAAYAKDKTLVGMGKMIGVHGFM
                     DPSTYDQSRAMKCCYHVLTTDGRMMPFCNYNIFHREKDMQRRNALARA"
     gene            7174..8931
                     /locus_tag="AC162G_08635"
     CDS             7174..8931
                     /locus_tag="AC162G_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR5075919.1"
                     /translation="MRVLLINPPRPAHSLWNPDAARLPPLALEYLAASPPPEAEVRIL
                     DAEADGLDASDVVGEAEAFQPDWVGLTALALPWVATAVALSRQLKQAGLSAPIAMGGA
                     ATTAVAPEIIASGCVDFVVQGEGEIAFRQLVSGADPRQVAGLCYRDDGAVVSTPAASP
                     LPDLDAHLPARICDRARYTAFGKRLDAIESSRGSPTSGWRARSAASVVGEIADLVSRG
                     AELIMVLDDHFMADIGRVRHICEELIQRQVRPWMWCHADVLAIRQQPEIVAKMREAGF
                     VVQLLTVQPCDVADASRRDNLRQAVQALHECDVALWATLLIDGLQPDRAQRWSMIEFA
                     RELDLEFAQIGFATPYPGTPLYDECRRKGLLLAHDWEDYNPHHRVCGEDVSPESAYAT
                     LRELYKAYYLRQEFALPRMWTRLRAENPHLAKLIQVFNPAGIKTARDVVREGLLKSAW
                     PTLLHHYCVPATLAEWSELIAVLKPLVNAHLAQQFPAYSTTVRMDTDIGSGSLAIVQG
                     EVQAFSAQADGADVALRLDNAALRRLLIGLGADPGPTLNGAHTAGSPEALSDFQRYVE
                     ALQTYLRSWCTCGATVEPV"
     gene            8918..9349
                     /locus_tag="AC162G_08640"
     CDS             8918..9349
                     /locus_tag="AC162G_08640"
                     /inference="COORDINATES: protein motif:HMM:NF046932.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApeP family dehydratase"
                     /protein_id="HIR5075920.1"
                     /translation="MNLYSADVLAVLPHRPPMLRVDGVLAIEPGRRGVARNVISIGEP
                     LATSAPDWSGVWLIEAIAQAGRLTASLGTDAAGRAGYLASIENFHIVRPAGPGDALTL
                     EVEVAAGRRGFVCVRGQVKAGDDLVAEGEVVLSMPRDESAR"
     gene            9379..10641
                     /gene="fabF"
                     /locus_tag="AC162G_08645"
     CDS             9379..10641
                     /gene="fabF"
                     /locus_tag="AC162G_08645"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR5075921.1"
                     /translation="MSRRMVVTGMGILISLGHEVDEVWTALLEGRSGVRRIQSFDPTP
                     FDSQIAAEVRDFDAAVCCSRKEARRLDRFVQYALYAADRAVADAGLKMDEVDAERVGV
                     LIGSGKGGTITLCDELRTLDRYGPERVSPFLIPMMICDMASAHVAMRVGAKGPNFAIV
                     SACCTSAHSIGEAAEVIRRGDADVMIAGGSDASLHPICVAGFTAMRALSRRNDDPVHA
                     SRPFDAQRDGFVIGEGSGVLVLESAEHAQRRGARIYGELLGYGATADAHHITAPVEGG
                     DGLVRAIRIALNKAGLAPEDVHYVNAHGTSTPLNDRVETLALKGVFGEYAYDLPVSST
                     KSMTGHLLGASGAVEAIFCLRAINDGIIPPTINYEYRDPACDLNYVPNQPLVRRVDVA
                     LSNSAGFGGHNASLIFGKWRNGHGAAPA"
     gene            10671..11912
                     /gene="fabF"
                     /locus_tag="AC162G_08650"
     CDS             10671..11912
                     /gene="fabF"
                     /locus_tag="AC162G_08650"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR5075922.1"
                     /translation="MTRRVVITGMGALTSLGPDVPSLWSALCAGQSGVGLITKFDTSE
                     WDVHIGAEIKDFDPASILGAKQARRLDPFVQYAMAAAFQAVQETGLCFDNGLAERTGV
                     IIGSGKGGTTTLFEQFRVMLEHGPKRIHPLLVPMWISSMASSQIAMALGVKGPNFCVM
                     SACSTGAHAIGEAAEIIRRDDADVMIAGGTEASVGPLTVAGFSAMRALSRRNAEPTRA
                     SRPFDAWRDGFVLAEGAGVVILEELEHAQARGARIYAELVGYAATADAYHITAPPEDG
                     EGLARAMHLALRKARLSPQDVDYINAHGTSTSLNDAIETRAIKSVFGEHAQRVPVSST
                     KSMTGHLIAAAGAVEAMICVKAICDGVLPPTINYEYPDPECDLDYVPNTARQARVDVA
                     LSNSAGFGGQNACLILQRAAI"
     gene            12059..12850
                     /locus_tag="AC162G_08655"
     CDS             12059..12850
                     /locus_tag="AC162G_08655"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0030497 - fatty acid elongation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR5075923.1"
                     /translation="MLLSGKQGLIFGVANDHSIAWGIAQALHAEGATLGFSYAVPALE
                     RRVRPLARSLRAEFVEPCDVTRDADIATLFEKARQAFGTLDFLVHSVAFANRDDLNRP
                     YLDTSREGFHLALDISAYSLTALVRAAAPLMNPGGSVLTLTFHGSRQVMPHYNVMGVA
                     KAALEASVRYLACDLGPRHIRVNAISPGPIRTLASAGVTGFKTLFRTFEDIAPLRRRV
                     TIEDVGKTAVWLCSDLSGAVTGEVVYVDGGFNILGIRLPEPVKEA"
     gene            12850..13593
                     /gene="fabG"
                     /locus_tag="AC162G_08660"
     CDS             12850..13593
                     /gene="fabG"
                     /locus_tag="AC162G_08660"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR5075924.1"
                     /translation="MRLSGCVALVTGGTGEIGRAICLALAQEGASVAVNYVCDAASAD
                     RLVSDVAAAGGEGVAVCADVADAGAVRSMVKTVLDRWGRVDVLVNNAGVTADRLLLMM
                     SDEHWDHVLDINLKGAFHCARAVAKTMMKKRAGRIINVASISGIMGQAGQANYSAAKA
                     GLIGLSKALARELATYRITVNVVAPGFIDTRMVKAIPAHVQEKFVQAIPLQRLGRPED
                     VAKAVVFLSSPAAEYITGHVLVVDGGLAM"
     gene            13608..14102
                     /gene="fabZ"
                     /locus_tag="AC162G_08665"
     CDS             13608..14102
                     /gene="fabZ"
                     /locus_tag="AC162G_08665"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR5075925.1"
                     /translation="MDIQDIFRLLPHRYPFLFVDRVLEVEPGRRAVAIKNVSINEPYF
                     AGHFPDHPIVPGVLLVEMVAQVGGIALRAGSNDDEATAYLGFLARIRNFRFRQPARPG
                     DSLRIEATVIRACLGVMVIQGRITTGGGTVAEGEVVIASPGDHTGERLRAERGSDERT
                     QELA"
     gene            14077..14520
                     /locus_tag="AC162G_08670"
     CDS             14077..14520
                     /locus_tag="AC162G_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR5075926.1"
                     /translation="MSERKNSLNTFERMRNVIVHTLNVAPDDVTLQARFREDLDADSL
                     DIVELIIAIEREFEADVFRYVDPSWEETLEARAAEISTVGQLVEYLDLLAPLCCSRPL
                     MSETRIHQRELEFRKELWRSPNPQKRVLALLMTANLHYGEIGWLC"
     gene            14514..15809
                     /locus_tag="AC162G_08675"
     CDS             14514..15809
                     /locus_tag="AC162G_08675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR5075927.1"
                     /translation="MLTHDASTRQNARRRVVVTGIGVVSPAGIGKEAFWQAIRQGRSC
                     AGPIRTLEPRPGQRVHIAASVDGFSINDYTDRKQARFMERVSGFAYAAAQMAMEDADL
                     TLTPSDSERAGTSIGTFAGPIRMARGEIERIQTGGIHKMNALSCIIGSSGASFGNITV
                     NFQLRGPGVTVYAAETAGMDALGYAYRQIKYDEADVMIAGGSEAPILHSSLILFDGLG
                     LLSRRNDQPERACRPFDAGRDGFVLGEGAAILVLEEMQHALRRGAHVYAELAGYAATC
                     LPANDLPAHDAPGDTSLAMRMALADAALAPDCVEFIAAHGIGTQAGDIRETLAIKQAL
                     GEHAYDVPVSSIKPIFGYTLGADGALQAASCCLALGRGLLPPTINYSQPDPMCDLDYV
                     PNQARAASPAVILHNSLSVLGKCGALVYKRWREGDPADG"
     gene            15802..17073
                     /locus_tag="AC162G_08680"
     CDS             15802..17073
                     /locus_tag="AC162G_08680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR5075928.1"
                     /translation="MARRVALTGVGIVSPIGIGKRDFWENLKAGRSGAGPIQAFDASG
                     YRSTMAAEVKVFDVRRYLDAAAVARWTDQADRANQFMLVATQQALADADVQLDAEDRT
                     RAGVSIGSAAGDLEARLPLLDDLFAAAARGDEAEAQRCARHVLTTFLGQVALSVNGMF
                     GLQGDSTVTAAACASSTDAVGYAFRQIQYGEADLMIAGGAEAPIQPYIFYSYAMLNVL
                     SQHNDEPERASRPFDRTRNGFVMGEGAGVILLEEWEHARRRGAHIYAEVAGYGASADA
                     YHMTAPSPTSEQSARAMRLALHEAGVQPDEVDYVCAHGTSTQLNDVNETKTIKNVFGE
                     AACRVPVSAIKSMLGHTQGAAGIMQAVTCALVMEHGFVPPTINYREPDPECDLDYVPN
                     VGRAIPIRTILQNTISFFGRNNAVVYRKPVA"
     gene            17070..18500
                     /locus_tag="AC162G_08685"
     CDS             17070..18500
                     /locus_tag="AC162G_08685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR5075929.1"
                     /translation="MRVLLIYTNREQSPYPVLPLGLAYVDASLRQRGIETRLLDLCFA
                     KDAAQVARDAVQTFKPDCIGLSIRNIDNLTYDAPVRYLPAIRPIVDACRQAFHAPIVA
                     GGSGFSIFPQAILRFLDLQIGIVGEGEEAFPLWLACGDPHRVPGVVTNRDSPGDRQPP
                     ARVRDLDALPIPTRDRAVSRTYELAGSAVNVQTKRGCPFRCLYCNYPVIEGRDVRLRS
                     PQNEGEELERLARAGVKKIHFVDAVFTYPPDHAAAICEEILRRRLDLHWVCGANPSSL
                     SPELACLMRRAGCSGVELGIDAANDGMLHRLRKGFTLDDVAACSEGCCQARLPFLFHL
                     IFGGLGETATTIEETLTAIGKMRATAVTYSFGLRIFPATGLAEVLQQEGGAFSDDDLL
                     EPRFYLSPELAPKMMERVLNHARREPTWLAPRQRDSAIAKAIMRLGTRLGTTGPAWRH
                     AAVGAPIKRAVAVVKAARTKRSVARA"
     gene            18559..19350
                     /locus_tag="AC162G_08690"
     CDS             18559..19350
                     /locus_tag="AC162G_08690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5075930.1"
                     /translation="MDKVAIVTGASSGIGEATARRLARDGMCVTLAARRREELERVAA
                     AIGDETRALVVPTDVRDRAAIQHMVQATLDRWRRVDVLVNNAGLSYPRRVIDLDPDQV
                     CEQIDVNLIAVIDCVQAVLPAMLRQSSGHIINVASLAGLIGIPGASIYCATKSAIVGF
                     SDALRREVQDSGIRVTVLSPGFVATNFSPRLRRAAEKLANGQRVRGVMCSDVVAERVA
                     RLLRHPRRHDILPTSWRPLIAFLRAFPGLADVVLSRYYVRMRERI"
     gene            19592..20920
                     /locus_tag="AC162G_08695"
     CDS             19592..20920
                     /locus_tag="AC162G_08695"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HIR5075931.1"
                     /translation="MEADLNWLDRQIALRDDVAYRKVGDSVYVYALRTGETSKLTGVG
                     ELIWGHLTRQTPLREIAASLSDQFAVPLAQVAQDVCKFVRSLYDRSIVTADGETGGVR
                     RSFREATRWQPELVITEVKRQRVPFRVDFETTYACNLDCVYCYTAKDNHRLLNTSEVQ
                     SILDQLAEAGTLFLCFTGGEPFARRDLEQLIRLAHERRFAILVLTNATLITPAYADLL
                     ARWCTVQVSLHGARPSTHDAFTAVNGSFRRACRGIEYLAQAGANVYVIFNATYHNVRE
                     EDALRRLCEQWQVGFALNVHLLPNVNGSLAPLDYRISDDWVLDLARQGKLVRRRSVCT
                     AATTKARISPFGDVYPCELVRRSFGNLREQAFEDIWRGAELEAFRASEFFCTPEACHA
                     CAWSDYCRRCPALAEMEDGNPNSPSSEACRVARLCAEAKQETYALERQPA"
     gene            21399..22814
                     /locus_tag="AC162G_08700"
     CDS             21399..22814
                     /locus_tag="AC162G_08700"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5075932.1"
                     /translation="MNDHDSGADGKVGQAWSHLKIADNLAYRFVGDEVFCWRTGAKVE
                     NRFAIVGGLGVEVFSSVERRAAFDAFRDEILARFDVSPDEFDSGLEEFLVSAVAAGLF
                     TEESPNGATSLSLRSPVSNGGEPEVSEADVLDTMFEKASAQVLPLKVFFELTHHCNHH
                     CQHCYLGFGVGVPGTSALSTSRQLELLDEMAQAGVLELILTGGEPTLHRAFTKILQRA
                     HELHFAVELLTNGTRLDEELVELLASVSLVEVRVPIYGLEAYHNRFVRNARSFKRTWK
                     GIVALHTHGVPVTVTSTLMSDNADQLLEIKRRLDSLGIRFQVSPLVYPTIQRNAAPTQ
                     YRATREQFRAMTPQLGITLRRSRCTAGVSRFRIGPDGSLHPCEMLSTIELGNLAHHSF
                     ETVLRSPERQEWIARFRSYLETKDRAGCRECRVRSYCVDCVGLSYLESTTFDGRCQEA
                     CQLAHAIAYIDQRDQMVAALA"
     gene            22861..23403
                     /locus_tag="AC162G_08705"
     CDS             22861..23403
                     /locus_tag="AC162G_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR5075933.1"
                     /translation="MEEYVERLCCDPASVNLSEANRLQRDLPQLIRLFYVLSREMLGA
                     LDITILQSYILMGLQVNGPMTMTEISDYTKLAASTVTRVADKLVKANLTERMCDERDR
                     RVVCLKLSESGEARSTQIRTLQNVFFQGLLSGLSALERESIVSGLALLTGRLAQFADQ
                     RHDAIDVGEDLLVMADTALA"
     gene            23466..24995
                     /locus_tag="AC162G_08710"
     CDS             23466..24995
                     /locus_tag="AC162G_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075934.1"
                     /translation="MDKRHEEKNAVGRLRQRDEFILSLAREAYQEKPFYRWFYDNASV
                     DVEHADVRRLPFITKKDFFHYEEETGQPYYLPLGYPDSSLEYLRTSGTTCQPLVIPWL
                     KSEWSFLYNSLLLNFIEQIKGRPRFLNAVNPNQLEEMRAFQSVLVNVPHDRMADAPYV
                     LDLIARHRPNIFLDNSPGLWFNYLLDHGLDPCRAGIQMVCAYPSAELAQKVESLGLKY
                     VSAFGCMEAGFFGYAHAGLGDVYHLSDAEPIRSASDSPTLLGGIRLQVFSRLFPCDCY
                     QGMDLQAMFGRRDIRVRRVDGELSTAGAGELVITCANSAFPFIKYLNSDRVEIHPEAK
                     CDCGFVGQSMRFECRDHNVQIPYLGGGMNVSLEGIALGLKGFRRVFPIHIAPDPTVRI
                     VVTFVEDELATAACLDLDLAERIMTAATGINSETMRLISSRRNFLPFFPAVRIPVGTI
                     PLRDQHSLKSRLYLDAVSGYVPDEYRPVLALIDQLVDRAAIIRSGSWFLPVEVSQPTP
                     A"
     gene            25375..26769
                     /locus_tag="AC162G_08715"
     CDS             25375..26769
                     /locus_tag="AC162G_08715"
                     /EC_number="1.17.98.2"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5075935.1"
                     /translation="MGSNGRAEQPTANELKRRISRHEAARSRTVGDDTFIISLREGAT
                     IQHRMDRVGTYIWQLADGTRTGEEMARAVARHYDADLAAVTDSVARFVHALHQSGLVT
                     LEGCTTPAFSRQNRRSNLPVPAQVARFYGAADEKCVPLVATLTLTHACNLKCYYCYDA
                     GQRGRALDFSHWRDILDQIAEAGTLCIRLSGGEPTAHPDFMLILEYAVQRGFVTSVVT
                     NGTLITPELAERLGRLALESVAVSFHGGSPDVHDRLAGRNGAFERSCHGVALLREHGV
                     KVIPGMVVTRRNFDDVDNFARLSATWGLSQPILSIAVYARTNGATDTLNDRLSLDQIR
                     NLMSRGWYRPFPNLYCGAARTKYAITPNGRVTPCQRLMIEVGDLERESFADIWRQSPA
                     LCQMREERWFADPDQCRTCPAYQKYCSRCPAQAYYEQGQLRATSSFDCQLVPVYLQLQ
                     QTTEVSAPTQPACL"
     gene            26828..28330
                     /locus_tag="AC162G_08720"
     CDS             26828..28330
                     /locus_tag="AC162G_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075936.1"
                     /translation="MNDGDEFILALARQAYQEKPFYRWLYDNAAVDVERAGVQDIPFL
                     TKSDIIRYEEETGQPYYLPVAYPDTQIEYRHTSGTTNRPITFPWLRAEWFFLCQSIFL
                     NFISQIKGSPRFLFCVNPDEEERAKAFGSVLVGVSRDRLSDAPYVLDLIERERPNVIV
                     DNSPGIWFNYLIEHGLEPRAAGIQMVLMLPNQELAHKIEAMGIQYGSVFGCTEGGLFG
                     YSHAGIGEVYHLSDADPATMLKLAISSQLGRLGGLKLQMLTRLFPCECYQGLDLQRMI
                     GRRDIRIQAENGRMEKTGFGELAITCANSVFPIVKHLNADMVEISPAAECACGFSGRS
                     MRFHHRDHEVQVPYIGINGSTYNLQWIAQELKGFRRVFPVYYALNGGFPRIVVTFLED
                     DTPDVPRVDTGMAGHILAACCGKSEAEVRETHAFAKQRFFFPAIRVPPGAIPIRDGAS
                     LKSRLYLDTVSGRAPDEYQPLLALVERLIDPSPIIRAGSWFLPVEQPVEG"
     gene            28330..29283
                     /locus_tag="AC162G_08725"
     CDS             28330..29283
                     /locus_tag="AC162G_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5075937.1"
                     /translation="MSDGIRTHKLTKRYAGNVLALAELDLDVAAGEIFGLLGPNGAGK
                     TTAVRILTTILRPSSGTASVGGYDVRKQPAQVRQRIGVVAQNACLDCMLTAYDNLDFY
                     AKIHGLRGAERAQRLREAFRWANLDGKERHLVDSFSGGMKRKLDLARVMMLSPQFVFL
                     DEPTAGLDPASRRSVWDMVGEMKARGCTVILTTHYMEEADRLCDRLAILHRGRKVAEG
                     APHEIRSQVSGKSSLEVHAPNLTDAMLIPLVRAGEIEKYVIVGNYHQRIARLYLDDAV
                     GKMPYVLNYLHDHGVTANAVTVQGPTLEDAFINLTGMRIEA"
     gene            29285..30064
                     /locus_tag="AC162G_08730"
     CDS             29285..30064
                     /locus_tag="AC162G_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5075938.1"
                     /translation="MLGAFDVWQRDLKLIRKFWVFFAVLLLDIVLLLLAFGPAMTRLL
                     PRFAYPAGSTTFQIDYFTFFVPGLVVLVVMQASLYMGEMSYIDRDMGVLEILFTAPMS
                     RWSICLGKLASATTVAAVIAGTLYVVSFALGFRPYDVGIGSAVVLLIILTASWGFAGC
                     SIATVMQIRSTSRWLLLLGAILSPLTAAAALQYPVQVMPEWIRPIAGANPLTHAVDMV
                     RAAALEGRWPDITSLIGLFGFAIVGTLLAVTSMKHSRLLGR"
     gene            30097..30870
                     /locus_tag="AC162G_08735"
     CDS             30097..30870
                     /locus_tag="AC162G_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5075939.1"
                     /translation="MEVWYVWQRDVRLMRRMINSFTMTILGTLVALAIFAPTIDRLVA
                     GIAFGTASVPYLQFYIPGSLILAAFSGSFYGGGSIQHDRREGVLQVMAASALSRRALA
                     LGKIASAVTRSMLIVLGVFAAVTAAGFRFIHPLAGALGLIGMAVLISWSFAAISSCLA
                     FVIRHRFGYDLLITGLIAPLSYLAAITYPETVLPDTLQVLARLNPLTYAANGVREIIV
                     YGTFPSALNLLMPLGLAGISTVLAARMARNLSGEKLWAP"
     gene            30889..33435
                     /locus_tag="AC162G_08740"
     CDS             30889..33435
                     /locus_tag="AC162G_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5075940.1"
                     /translation="MSILLTMAWRALWLHKLRTVLTIMGVMLGVAVILAVRTANRSTL
                     RSIETLFDEAAGKSSLTVESAVSSRYAQIGFSDDTLRQAQTVAGVVQAVPRLTASTVF
                     QGKKGPVELSVQGVDPNVDQEVRIYPLVDGSFLPTAGRQRAILVVEDFAEREGIRVGR
                     DIELLTPSGLETFRVAGILARKNAGVTSGGRVVFVSLRIAQEAFQRGRKIDALDVVVK
                     PDIAQSPVALQQFKEQLQTALGDDYIVLYPAAKGSNVAEALSSVQYSLELFGLFALFA
                     SALLIHTTFTMAIAERMREVGMLRMLGTSHAQIVGQLLGGALLLGAIGVGLGLIMGFV
                     MAVPLLGFFASLTGYGLGTLSPSPGDILLSGGLGLIVTLLSALWPVIRAGRLSPLEAV
                     RVRGQTTEGRSIGRSWVVGLALLAIWGASFFIPQLPSAPMFIVFFGGATLLLPSVVYQ
                     ASKRLRRPVATVYGYEGSLGATNLERSRWRTSTTVAVLMIGVAMGVSIGAVSDSLTTS
                     IRSWVDSAMGADLFISSYSSMDPLWGRRLAAIDGVDVVSPLRILDARVEDPTRAGHYF
                     TARVQAIDPATLKQIGEQSYVSGQGAPADLWAAFEQGGSLLVSTQYADRYDIRQGDQL
                     RLRTARGMQEFPVTAVILDYNSMRGTVMISQDDLRRYFGLNDADVYLLKIATGADVEA
                     VRQRIDAQYGRSGQLTIQSNSEVRRSLLQSVGSIMLAFNAIVVIAFIIAAFGIANTLT
                     MNAIERTREIGMLRAVGMTRFQVGKMVLAEALVMGVIGGVFGLAFGGLFSRVVVWWMS
                     SGIGFRVDYVWPVLAVLISGALVLLVSQLAAFLPARRASGYAIVEALRYE"
     gene            33490..>34121
                     /locus_tag="AC162G_08745"
     CDS             33490..>34121
                     /locus_tag="AC162G_08745"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5075941.1"
                     /translation="MPILEAHDVSKQYKLSEQVTISALTHVNFHADRGELVAIRGPSG
                     SGKSTLLHVLGGMDRPTSGEIALDGQPLSRLSDRELTLLRRRKVGFIFQFFNLIPTLN
                     TEENILLPLLIDGKRKTQCLPRLDEIVSLVGLDHRRQHRPEQLSGGEQQRVAIARALI
                     AEPSLILADEPTGNLDSAMGLEIIELLKRLATTYAQAIVVVTHDPRVAEHA"
BASE COUNT         5992 a        10396 c        11113 g         6620 t
ORIGIN      
        1 cctcgaagtc ttttacggcg agtttcaggc gctgttcggg gtgagtctgg aggtaaccgc
       61 cggagaaacc atcgccatca tcggcgcgaa tggggccggg aagacgactc tgctcaacac
      121 gatcgccggc gtgctgaccc cgcgccgggg gcgggtgatc tatcgaggcg aggtggtttc
      181 cggctctccg tcatatcggc tttgccggcg cggcatttcg ctggtgccgg agggccggag
      241 aatatttccc agcctgacag tggaagagaa tctggaagcc ggcgcgtatt ccggccggcg
      301 cggcccctgg caacgagatg ccatctatga cttgtttccc atcctgcgcg accgggcgcg
      361 tgcccccggg acggaacttt cgggcggcga gcaacaaatg ctggcgatcg gtcgggccct
      421 gatgagcaac cccgacctga tcttgatgga cgagatttcg ctcgggctgg cacccgtgat
      481 cgttcgagag ttgtataaag tcgtacgcgc ggtggccgaa gcgggcgcga ccgtcattct
      541 ggtcgagcag gacgtgagcc gcggcctcaa agccgccgac cgagtttatt gcctgctgga
      601 gggtaaggtc agcctggcag gccgcccagc agagttgagc ctggctcagg tctctcaggc
      661 ttacttcgga gtctgacgat gcagtgggtg aacatcgttg cacaaggagt cttgctggga
      721 ggactgtatg cgctctacgg gacggggttg tcgctcatct tcggggtgat gcggctggtg
      781 aacctggcgc acggcgacat gatcgtggca gcggcatatc tggcgctgct cgccgggcaa
      841 ttcctgggct tgtctccctg gctgagtttg ctgctggccg cgccggcact ctttatcatc
      901 ggcgtcttgt tgcagcgcgg cttgctcaac gcgactctcg gaaaaggcgt actgccgccg
      961 ctcatcatca cgttcggact ttcggtgatt acccagaatg tgcttcagct aggcttctcg
     1021 gcagatacgc aggggctgga cgccggcccc gttgaggccg cgagtatcga ggtggtgaag
     1081 ggtgtgacgc tgggtgttct gccgttgatt gtgttcgcca cggcactcgt cgttcttgca
     1141 gggttgcacc tgttccttac acgcacgaag ctgggcagag cgttccgggc cacgtcagat
     1201 gatcccacca cggcagagtt ggtcggaatc aacagccgcc aggtttatgg ctgggcgatg
     1261 ggcctgtcgc tcgccatcgg ggcggtgggc ggcgtgctct tgggaatccg cacgacgttt
     1321 gcaccgacgg atgggccggc ccggttgatc ttcgcttttg agacggtcat catcggcggg
     1381 ctgggcagcc tgtggggaac actcgccgga ggcattatcc tgggggtggc ccagaacatt
     1441 ggcgcgcagc taagtccggc ctggtttcaa ctggccgggc atttgatcac actggcgttg
     1501 ttggcgttca gacccaccgg cctgttcgcg cgtacacggg ataccgagtg agccatggga
     1561 gcgccgacgc caaccccgga gtcatcccca gcgaccgacc accccaaagc gcgccccata
     1621 tccccgcgtg ggcttgccct cggcagcgtc cttctgctga cggccctgtt ctccttgccg
     1681 tggtggggca cgccggacca gatgcgcacc ctcgtcgagt tcatcacctt cctggcgctg
     1741 gcgcagatgt ggaatctgct ggcgggttac ggcgggatgg tgtcgtttgg ccagcaagcc
     1801 tggattgggc tgggcggcta taccctgatc gtcctggcgg acgacctgca cgtgaatatg
     1861 tttctggcgg tgatcgtggg gggcgtggtg gcggcgctcg tcgcgctgcc gactgccgcg
     1921 ctcgcctttc gcctgcgggg tgggtatttc gcggtcggaa cctgggtgat cgccgaggtc
     1981 tttcggctgc tagtcgccag ttccaccgaa tggctgaaag ggggacttgg ccgctctctt
     2041 gcggcggcgg ggttgatggc gcgccccctg cgcgagcagt acacgtattg gctggccatc
     2101 gtcattgggg ccggggcaat cgcactcgtc tattatctca tgcgttcccg tgtggggttg
     2161 ggcctcaccg ccattcgaga ttccgagagc gcggccatga gtgtcggcgt gaagatcacg
     2221 cgcatcaagt tgctggtctt cgtgatcggc gcctttggca ccggtgttgt cggcgggctg
     2281 atttacttga acgcgctccg gattacgcct caggcatctt tcagcatcca atggtcggcc
     2341 ttcatggcgt tcatggtcgt catcggcggg ctgggaacgg tcgaaggccc gatcgtcggc
     2401 gcggtcgtct ttttcgtcct ccggcattac ctgtccagtt tggggccgtg gtcaatcatt
     2461 cttcttggag gcaccgccgt catcatcgtg cttgtcgcgc cggacggctt atggggcgtc
     2521 ctgcaccgcc gcttcgggct tgagcttttc acgattcggc gacggttgcc cgccgcgatg
     2581 gcccaggggc aactcgcaat ggtggaaggg aagcctgctt cgagggatcc cgcgctcggc
     2641 gaaccgccat cctgaacggg cgtggacgtg gacaagtgat tcgatcttga ccggggaggc
     2701 gatatgtcta tccagatagc cgacgtcaag cagatcgtcg tcgaggcggg acgactggcc
     2761 ttgctctact atggaaaagt gtccaagtcg gtgaaggccg atttgagtgt tgtcagcgag
     2821 gccgatgcag ccgtcgaaaa cttccttcgg ttgtcgctca ccgcgcttgc gccgcagtac
     2881 ggctatatag gcgaagaaac cgaagagtcg cggccgcctg cgccggggga agagcacgcc
     2941 tgggtcgtgg atgcgctgga cggcagccgg gcgttcgtgg cgcaaatgcc gctctggatg
     3001 ccagcagtat gtttgatgca aggcaatcag ccagtggccg gcgccgcctt caacccggtc
     3061 accaacgagt tgttctgggc cggcgaagac ggcccggctt tctgcaatga cgaaccgctg
     3121 cacccgacgt tctcctcggc gctggagcct aacacgttca tttttggccc gacgaatcat
     3181 catcgaacgt tcacgatcga ttttccgggt cgaatctatt gcctgggcgc gccgatttat
     3241 cagttgtgtc tggtggcgaa gggcgcggtc gccgcgttgt ttttcgatcc ggcgatcaac
     3301 ctgtgggacc tggcgctgcc cagcctgctt ctggagcgca caggggcggt gttggtttat
     3361 gcatctggcc ggccggtgtt tctttctgaa ctcatgggac gcagtaaagt accggagccg
     3421 attttcactg gaggggccaa agtggttgag atcctgcgac agcggatcac atataaacat
     3481 gcttagcggg gaagcggcgg aacatccggc gtgcccgatc tgcgcaacat gcgcctcctg
     3541 cccaagccga tcatcgccgc cgtcaatggg ctggccgccg gggcgggcgc tgtcatcgcc
     3601 cgcgcagccg atctgagggt cgtggccgat acggctagat tcgctttcct cttcacgcgg
     3661 gtaggtctga ccggcgccga taggggtgca gccttcttgc ttccgcgcgt ggtcgggcag
     3721 ggccgcgcga tggatctatt gatgctgggc ggcgacgtag acgcgccgac ggctgaacgc
     3781 catggcctcg ccaatcgagt ggtcctcgcc gatcaggtgc tggccacggc gcacgacctt
     3841 gctgagcggc tgagtgccgg cccgacgtac gctactggca tgaccaagct gatggtgaac
     3901 aacgagtgga cgatggacct caacgccgca ctggaggcgg aagcccaggc gcaggcgctg
     3961 atgatgatgg gcgaagacca tcgcgctttt tacgaagcgt tcaaagagaa gcgcaagccc
     4021 gacttcaagg gaagataagc gctgtaataa ccaacctatg gctcggtgct gatttcgaca
     4081 aatgccgtca taccccagcg cagcgccgga tcggttgcgg ccagctcgat ccatacggtg
     4141 aagttggcag tgccctgccc gggcgtggac atcgggctga tgcgcgtgac agtgcccgtg
     4201 agcgccatcc ccggcagcgc atcgaaggta acgagagccg tttgcccttc gcgcacgcgc
     4261 gcaatgtcga tctcgttcag gtcggtagtt tccacgtgta gacggttcag gtcgcccaac
     4321 gtcactaccg gctgacccgg cgctgcaagt tcaccggcgc ggacggacac agcgccgacg
     4381 ctgccgtcga acggtgctac gatctgcaac ctggacaaac gcgcctgtgc cgcatcgaca
     4441 gccacctgag cctgcgcgac agtggcctgc gcaacggaca actcctcctc agaagagcct
     4501 gcctgcagaa gctcgacctg cgctctggcc gcgtcgcgct tggcctgcgc agcggtgact
     4561 tgcgcccgca aggcattcag ctcgttctct gccgcaccgg atatcagttg gttcacacgt
     4621 tcctgagcgg cacgatgcgc ctgcctggcc gcctcgagcg ccgcgcgagc ctgctcctcc
     4681 ggcgtgcccc cgatgtcttt gacccggtcg taggtgtcct gcgcgacttt caggtcgcgc
     4741 gcggctgcgg ccagcgccgc ctgggcagcc gcgatctcgg cggccggcgg gccggcctgc
     4801 gcctgcgcta attgcgccga agctgcccgc agctccgctt cagccgattg aaccgcctgc
     4861 tcggcgattg cgatctgctc ggagcgcggg ctggctgtct gctgcgctaa ttgtgcctgg
     4921 gccagtgcca gcccggcctc ggcttgcgcc agggccatct gcgcatccac atcgtccagc
     4981 cgcaccaaca cgtcgcccgt gtggacgacg tcgccaggct gcgccagcac ggcctttacc
     5041 gcgccactca cttgaaagct gagcacagcc caccgctccg gcatcacctt gccggaagcg
     5101 gacacagtat ccggcgcgat tcccggtgac gctgctgggg cgggttgaat ctcgcgctgg
     5161 gcggccgtgc tttcctcgca ggccgcgatg agcatggata tcacgacaat cctcataagg
     5221 ttgacggctt tcatcagttt ttctccaggg tacagcctgt ccgcttcggc actcccgaaa
     5281 acctctcgtg ctcatcgtac ccaaaccgca caggcggaca caaccgcgcc caggtgagtt
     5341 tcgacctcga ctgcaatcta aaactaggct gtagcgatct aaactccggt cgaggttcaa
     5401 actcgacttt ccgctgttgc ttgcctgcgc cgcatcgcat actctcttca aagacagctc
     5461 cgagtcgtcg ttgccattcc gctaataaca cgaagatggc tgaactctac tcgccggcaa
     5521 gggtgtgacc cgccgtttcg atccgagttc gatagctatt cccgttccgg cggaatcgcg
     5581 cagttcacct gcagttgcca ggaactcgct gtgccaaaca ggaggaacca tgagtacgac
     5641 cgtccccgtc gacgcgccgg ttgtcccgct aggcgctcaa gtccatgacc cggttcaagc
     5701 ggcaatcgag cgagcgaata ccagacagga tcaggaccac atcttcttcg gcatcacgcg
     5761 tagcacgtgt ccggagtgcc tggcgctgat caacgcgcag atcctcttcc gcgacggcca
     5821 ggtgttcatg cgcaagtttt gcccggagca cggtcacttc gaggcgctca tggctgccga
     5881 cgcagactgg tggctgcacg cgctcagccg gaccaagcct ggcaccattc cgctgtggca
     5941 cgccacacag cgcaaaacgc agtgtcccga ggactgcggc ctgtgcgacg atcaccagca
     6001 gcacacctgc tatcctctga tcgagattac cgatcactgc gatctgcatt gcccgatctg
     6061 cttcgcccgg aatcagcatg cctggtatct gtcgccggag caaatgacct tcatgctcga
     6121 tcgtctggcg caggccgagg gccgcgcgga gatcgtgtgc ctgaccggcg gcgagccgac
     6181 cgtgcatccg caattcttcg agctgctgga cattgcgatc aaccatcccg tcgccaccaa
     6241 ggtggccgtc aacacgcacg gcctgcgcct cgcccgcgac gaagagttcg tccgccgcat
     6301 cgccgagacc ggggcctaca tccagttgca gttcgatggg ttcaaagcgg agacttacag
     6361 gcggctgcgc ggcgccgatc tcagcaagat gaagcgcttg gccttcgacc atctggctaa
     6421 gtacaacgtc aagacgctgc tgatcaccac catcgcgcaa gacctcaacg aggatgccgt
     6481 cggcgagacg attcggctgg tgttggagaa cgacttcatc cactcgctga cgctgcagcc
     6541 gctgatctat tgtggcgacg cgctctccaa ccatctgccg cacgacatgc agcgtcacat
     6601 cacactgacg acgatcctga acctgctcga ggctcagatg gacggcttga tgaccaagga
     6661 ggattttgta tccatcccgt gctcgcaccc ctcctgctcg gcggtgggct acttcctgaa
     6721 ggccgggccg aacgattggg tctcgctggc ccgcatcacc caggccgaca agatgctgaa
     6781 ctacctgcaa aaccggctgc tgttcaacag cgagcagtgg atgcgcgatc tgcaatcgaa
     6841 cctggagacg ctatggtcag cctcggcggt gatggagtcc gagaaagtgg gacgggcgct
     6901 cagctgctgc gcggcggctt acgccaaaga caagacgctg gtcggcatgg gcaagatgat
     6961 cggcgtccac ggcttcatgg atcccagcac ctacgaccag agccgggcga tgaaatgctg
     7021 ctatcacgtc ctcacgaccg acgggcgcat gatgccgttc tgcaactaca acatctttca
     7081 tcgtgaaaag gacatgcagc gccggaatgc gctggcccgc gcctaatgac tcgccgtgcg
     7141 acgccctgat acttcggacg ggtaggccca gcgatgagag tccttctgat caatccgccg
     7201 cgaccggcac atagtctgtg gaatccggat gccgcccgcc ttccaccgct ggcgctggag
     7261 tacctggctg ccagcccgcc accggaggcc gaggtcagaa ttctggatgc tgaagcggat
     7321 ggcttggatg cgagcgacgt ggttggagag gcggaagcct ttcagccgga ctgggtgggc
     7381 ctgaccgcgc tggcgctccc ctgggtggca accgcggtgg ccctatcacg ccagctcaag
     7441 caggccgggc tgtcggcgcc gatcgccatg ggtggcgctg cgacaaccgc cgtcgcgccg
     7501 gagatcatcg cgtccggatg cgtggacttc gtcgtccagg gtgagggcga aatcgcattc
     7561 cgccagctgg tatcgggcgc ggatccccgc caggtggccg gattgtgcta tcgggacgac
     7621 ggagcggttg tcagtacgcc tgctgcgtcc ccgctgcccg atctcgacgc gcacctgccg
     7681 gcccgaatct gcgaccgcgc ccggtacacg gccttcggca agcggctgga cgctatcgag
     7741 agcagccggg gcagcccgac gagcggctgg cgggcgcgct cggccgctag cgtcgtcggc
     7801 gagatcgccg acctggtgag ccggggtgcc gagctgatca tggtgctcga cgatcacttc
     7861 atggccgaca tcggccgtgt gcgccatatc tgcgaggagc tcatccagcg ccaagtgcgc
     7921 ccctggatgt ggtgtcatgc cgatgtcctg gcgatccgac aacagccaga gatcgtcgcc
     7981 aagatgcgcg aagcgggctt cgtcgtacag ttgctgacgg tgcagccgtg cgacgtggcc
     8041 gacgcatctc gccgcgacaa cctacgccag gccgtccagg cgttgcacga gtgcgatgtt
     8101 gcactgtggg cgacgctgct gatcgacggg cttcagccgg atcgcgccca acgctggtcg
     8161 atgatcgaat tcgcgcgcga gctcgatctc gaattcgcac agatcgggtt cgccacaccg
     8221 tacccaggca cgccgttata cgacgagtgc cggcgcaaag ggctgctgct cgcgcacgat
     8281 tgggaagatt acaacccgca tcaccgggtg tgtggtgaag acgtgtcgcc cgaatcggcc
     8341 tacgcgacgc tccgcgaatt gtacaaggcg tactacttgc gccaggagtt cgcgctgcct
     8401 cgcatgtgga cccgtctgcg cgccgagaac cctcatctgg ctaaactcat ccaggtgttc
     8461 aatccggcgg gtatcaagac tgcgcgcgac gtggtgcgcg aaggcctgct caagagcgcc
     8521 tggccgacgc tgctgcacca ctactgcgtc cctgccacct tggctgaatg gtcggagctc
     8581 atcgccgtgc tcaagccgct ggtcaatgca cacctggcgc agcagttccc tgcgtactcg
     8641 acgacggttc ggatggacac agacatcggt tctggaagcc tggccattgt gcagggcgag
     8701 gtccaggcgt tttcggcgca ggcggatggg gccgacgtgg ccctccggct ggacaacgcg
     8761 gcgctgcgcc ggctgctcat cggtctcggc gcggatcctg gcccaacgct caatggcgcg
     8821 cacacggccg gatcgccaga ggccttgagc gactttcagc gctacgtgga ggcgctccag
     8881 acttatctgc ggtcgtggtg cacatgcgga gcaaccgttg aacctgtata gcgccgacgt
     8941 gctggccgtc ctgccgcacc gaccgccgat gctgcgcgtc gacggcgtgc tcgcgattga
     9001 gccgggccgg cgcggcgtgg cccgcaatgt gattagcatc ggcgagccgc tggcgacgag
     9061 cgcgcctgac tggtccggtg tgtggctgat cgaagcgatt gcccaggcgg gtcggctgac
     9121 tgcgtcgctg ggaacggatg ccgccgggcg cgccggctac ctggcgagca tcgagaactt
     9181 tcacatcgtt cgcccggctg ggccgggaga cgccttgaca ctcgaagtcg aagtcgctgc
     9241 tgggcgacgt gggtttgtct gcgtgcgcgg ccaggtgaag gccggcgacg acctggtggc
     9301 cgaaggtgaa gtcgtcttga gcatgccccg cgacgaatcc gcacgttgac ctggccgacc
     9361 tgattggaga tcgggcgtat gagccgacgc atggttgtca ccggcatggg tattttgatc
     9421 tcgctggggc atgaagtgga cgaagtgtgg acggcgctgc tcgaaggtcg ctccggcgtg
     9481 cggcgcattc aatcgttcga tcccacgcct ttcgacagcc agatcgccgc cgaggtacgc
     9541 gacttcgatg cagccgtctg ttgcagccgc aaggaggccc ggcgactcga tcgatttgtg
     9601 caatacgcgc tgtacgcagc cgaccgcgcc gttgccgacg ccgggctgaa gatggacgag
     9661 gtcgacgccg agcgggtcgg cgtgctgatc ggttcgggca aaggcggcac catcaccctg
     9721 tgcgacgagc tgcggacgct cgatcgttac gggccggaac gggtcagtcc cttcctgatc
     9781 ccgatgatga tttgcgatat ggcgtcggct cacgttgcca tgcgggtggg cgccaaggga
     9841 cccaacttcg ccattgtctc ggcgtgctgc accagcgcac attcgatcgg cgaggccgcc
     9901 gaggtcatcc ggcgcggcga cgccgatgtg atgattgcag gcggcagcga tgcctcgctc
     9961 caccccattt gcgtggccgg cttcacggcc atgcgcgcgc tctcgcgtcg caacgatgac
    10021 ccggtgcatg ccagccggcc gttcgacgcc caacgcgacg ggttcgtcat cggcgaaggg
    10081 tccggcgtgc tcgtgctcga gtcggccgag cacgcgcagc gacgcggcgc gcgcatctac
    10141 ggcgagctgc tgggttacgg cgcgacggcc gacgcgcatc acatcaccgc tccggtggag
    10201 ggtggcgacg gtttggtgcg cgccatccgc attgcgctga acaaggccgg cctggcgccc
    10261 gaagacgtcc attacgtcaa cgcgcatggc acgtcgacgc cgctcaacga tcgcgtcgag
    10321 acgctggcgc tcaagggcgt cttcggtgag tacgcttacg acctgccggt cagctcgacc
    10381 aagtcgatga ccggtcatct actgggcgca tccggcgcgg tggaggccat cttctgcctg
    10441 cgcgccatca acgacggcat aattccgccg acgatcaact acgagtatcg ggacccggct
    10501 tgcgacttga actacgtgcc caaccagccc ctggtgcgcc gcgtcgacgt agcgctgtcc
    10561 aactcggccg gattcggtgg gcacaacgcg agcctaatct tcggcaagtg gcgcaacggg
    10621 cacggcgccg cgccggcttg agttgcgttt gagaaagaag gtgagatgac atgactcgac
    10681 gtgtggtcat caccggtatg ggagcgttga cgtcgttggg gcccgacgtt ccgtcgctgt
    10741 ggtcagccct gtgcgcgggg caatccggtg tcggcctcat cacgaagttc gacacttcgg
    10801 agtgggacgt ccacatcggg gccgaaatca aggacttcga cccggcgtcc attctgggcg
    10861 cgaagcaggc gcggcgactc gatccgtttg tgcaatacgc gatggccgcc gcgtttcaag
    10921 ccgtgcagga gacgggtctg tgcttcgaca acggcctcgc tgagcgaacc ggcgtgatta
    10981 tcggcagcgg caaaggcggc acgaccacgc tcttcgagca attccgcgtg atgctcgaac
    11041 acggtcccaa gcgcatccac ccgttgctgg tgccgatgtg gatcagcagc atggcttcca
    11101 gccagatcgc catggcgctg ggcgtgaagg gaccgaattt ctgcgtcatg tcggcgtgca
    11161 gcaccggcgc acacgccatc ggcgaggcag cggagatcat ccggcgcgac gacgccgacg
    11221 tgatgatcgc gggcgggacg gaagcctcgg tcgggccgct gaccgttgcc ggattcagcg
    11281 ccatgcgcgc gctctcacgg cgcaacgccg agccgacgcg cgccagccgc ccgttcgatg
    11341 cctggcgcga cggcttcgtg ctggccgagg gagcgggcgt ggtgatcctg gaagagcttg
    11401 agcacgcgca ggcgcgcggc gcacgcatat acgccgagct ggtgggctat gcggcgacgg
    11461 ccgacgctta ccacatcacc gcgcctccgg aggacggcga gggcctggcg cgggctatgc
    11521 atctcgcgct gcgcaaggcc cggctctcgc cgcaggacgt ggactacatt aacgcgcacg
    11581 gcacctcgac gtcgctcaac gacgcgatcg agacgcgcgc catcaagtcg gtgttcggcg
    11641 agcatgccca gcgagtgccg gtcagctcga ccaagtcgat gacaggccac ctgattgccg
    11701 ctgccggcgc ggtggaggcg atgatctgcg tgaaagcgat ctgcgacggc gtgctgccgc
    11761 ccacgatcaa ctacgagtat ccggatccgg agtgcgacct ggattacgta ccgaacacag
    11821 cgcgtcaggc ccgggtagat gtggcgctct ccaactcggc aggcttcggc gggcaaaacg
    11881 cgtgcctgat cctgcaacgc gcggcgattt agagtgtgtc ttaagactcc gtgcagccgt
    11941 cactctgggc gggcgcagcg agcgaagtgt ctcatgtcgg gccgcgaaaa cagagattct
    12001 tcggcgcttc gtgcctcaga atgacatgtc ggatgcactg tgagagcgag gaggcgctgt
    12061 gttactcagc ggcaagcaag gcctgatctt cggcgtggca aacgatcaca gcattgcctg
    12121 gggcatcgcc caggctctgc acgcggaggg cgcgacactc ggcttcagtt acgccgtgcc
    12181 cgcgctggag cgacgcgtgc ggccgctggc gcgcagcctg cgggccgagt tcgtcgagcc
    12241 gtgcgacgtg acgcgcgacg cggacatcgc gacgctgttt gagaaagcgc gtcaggcgtt
    12301 cgggacgctg gacttcctgg tgcatagcgt cgccttcgcc aatcgcgacg atctcaaccg
    12361 gccgtacctc gacacctcgc gcgagggttt tcacttggcg ctcgacatca gcgcctattc
    12421 gctcacggct ctggtgcggg ccgccgcgcc gttgatgaac ccgggcggca gcgtcctcac
    12481 cctcaccttc cacggcagcc ggcaagtgat gccgcactac aacgtgatgg gtgtggccaa
    12541 ggccgcgctc gaagcgagcg tgcgctatct ggcctgcgac ttggggccgc gccatatacg
    12601 cgtcaacgcc atcagccccg ggccgattcg cacgctggct tcggccggcg tgaccggttt
    12661 caagacgctg tttcgcactt tcgaggacat cgccccgctg cggcggcgcg tcaccatcga
    12721 ggacgtgggc aagacggccg tgtggctgtg cagcgatctg tccggcgccg tgaccggcga
    12781 agtggtgtat gtcgacggcg gcttcaacat cctgggcatt cgcctgccgg agccggtgaa
    12841 ggaagcgtga tgaggctatc gggctgcgtg gcgttggtca ccggcggaac cggcgagatc
    12901 ggacgcgcta tctgtttggc gctcgcccaa gaaggcgcca gcgtggccgt gaactacgtg
    12961 tgcgatgcgg cgagcgcgga tcggctcgtc tccgacgtcg ccgctgccgg cggtgagggc
    13021 gtcgccgtgt gcgccgacgt ggcggacgct ggtgcggtca ggtcgatggt caagaccgtg
    13081 ctcgaccgct ggggccgtgt cgacgtgctg gtgaacaacg ccggggttac cgccgatcgg
    13141 ctgctgctga tgatgagcga cgagcactgg gaccacgtcc tcgacatcaa cctcaagggc
    13201 gcgtttcact gcgcgcgtgc cgtcgccaag accatgatga aaaagcgggc tggtcgcatc
    13261 atcaatgtcg cctcgatcag cgggatcatg gggcaggccg gtcaggccaa ctacagcgcg
    13321 gccaaagccg gcctgatcgg tctgagcaaa gcgctcgccc gcgagctggc gacctaccgc
    13381 atcaccgtca acgtcgtcgc gccggggttc atcgacacgc ggatggtgaa agcgattccg
    13441 gctcacgttc aggagaagtt tgtgcaggcg attccactcc agcgcctggg ccggcctgaa
    13501 gacgtggcca aagccgtcgt cttcctgagc agcccggccg ccgagtacat caccggccat
    13561 gtgctggtag tggacggcgg tctggccatg tgagcgaggt ggacgccgtg gacattcagg
    13621 atatctttcg gctgcttccc caccgctatc ccttcctgtt cgtcgatcgc gtcctcgaag
    13681 ttgagccggg acggcgcgcg gtggcgatca agaacgttag catcaacgag ccgtacttcg
    13741 ccgggcactt ccccgatcat cccattgtgc cgggtgttct gctggtcgaa atggtggcgc
    13801 aggtcggcgg gattgccttg cgggcagggt cgaacgacga cgaggctact gcctacctgg
    13861 gtttcctggc gcgcattcgc aactttcgct ttcgccaacc ggcgcggccc ggcgactcgc
    13921 tgcgcatcga agcgacggtg attcgagcct gcctgggcgt gatggtgatt caaggtcgca
    13981 tcaccaccgg cggcgggaca gtcgccgagg gcgaggtggt cattgcgtcg cccggcgacc
    14041 acaccggtga gcggctgcgc gcggagagag gttccgatga gcgaacgcaa gaactcgctt
    14101 aacacgtttg agcgaatgcg aaatgtcatt gtgcatacgc tcaatgtcgc gccggacgac
    14161 gtgacgctcc aggcccgttt tcgcgaagac ctcgacgccg attcgctgga catcgtggaa
    14221 ctcataatcg ccatcgagcg cgagttcgag gccgacgtgt ttcggtacgt cgatccatcg
    14281 tgggaggaaa cgctggaggc ccgcgccgcc gagatctcca ccgtcggcca gctcgtcgag
    14341 tatctcgacc tgctggctcc cttgtgttgc tcgcggccgc tgatgagcga gacccgcatt
    14401 caccagcgcg aactcgagtt tcgcaaagaa ctatggcgct ctcccaatcc tcagaagcgg
    14461 gtgctggcgc tgttgatgac ggcgaatctg cactacgggg aaatcggatg gttatgctga
    14521 cacatgacgc ctcgacacga caaaacgcgc gccgccgcgt ggtggtgacc ggcattggcg
    14581 tcgtgtcgcc ggccggcatc gggaaagaag ccttctggca ggccatccgt caaggccgct
    14641 cgtgcgccgg gccgatccgc acgcttgagc ctcggccggg ccagcgcgtg cacatcgcag
    14701 ccagcgtgga tggcttctcc atcaacgatt acaccgaccg caagcaggcg cgcttcatgg
    14761 agcgcgtgtc gggctttgcg tatgcggccg cccagatggc gatggaggat gccgatctca
    14821 ccctcacccc gagcgactcc gaacgcgcag gcacgagcat cggcacgttt gccggcccga
    14881 tccgtatggc gcgcggcgag atagagcgca tccagacagg cggcattcac aagatgaacg
    14941 ccctgtcgtg catcatcggc agcagcggcg cgtcgttcgg caacatcacg gtcaattttc
    15001 agttgcgcgg gccgggcgtg acggtctacg ccgccgagac cgccggcatg gatgcgctgg
    15061 gctatgcgta tcggcagatc aagtacgacg aggccgatgt catgatcgct ggcggcagcg
    15121 aagcgccgat cctgcattcg tcgctaatcc tgttcgacgg cctcggcttg ctgtcgcgcc
    15181 gcaacgatca gccagagcgc gcctgccggc cgttcgatgc cgggcgggac gggtttgtgc
    15241 tcggcgaggg tgcggccatt ctcgtcttgg aagagatgca gcatgcgctg cggcgcggcg
    15301 cgcacgtcta cgccgaactg gccggctatg ccgcgacgtg cctgcccgcc aacgacctgc
    15361 cggcgcacga cgcgccaggc gataccagcc tcgccatgcg catggcgctg gcggatgcgg
    15421 ccctggctcc tgactgcgtc gagttcatcg ccgcgcacgg catcggcact caagcgggcg
    15481 acatccggga gacgctggcc atcaagcagg ctctgggcga acacgcgtac gacgtgccgg
    15541 tcagttccat caagcccata ttcgggtata cgttgggcgc cgacggcgcg ctgcaagcgg
    15601 cgagctgctg tctggcgctg gggcgcggct tgctgccgcc gacaatcaat tacagccagc
    15661 cggatccgat gtgcgacctg gattacgtgc ccaaccaggc gcgggcagcc tcgcccgccg
    15721 tgatcctaca caacagccta tctgtcctgg gcaagtgcgg cgcgctcgtc tacaagcgct
    15781 ggcgcgaagg agatcctgcc gatggctaga cgtgtggcgc tgaccggtgt ggggatcgtg
    15841 tcgccgattg ggatcggcaa acgcgacttc tgggagaacc tcaaggcggg tcgctccggt
    15901 gcagggccga tccaggcgtt cgatgccagc ggctaccgct ccacgatggc tgccgaagtg
    15961 aaggtcttcg atgtgcgccg ctatctggat gccgccgccg ttgcgcgttg gaccgatcaa
    16021 gccgatcgcg cgaatcaatt catgctggtt gcgacccagc aggcgctggc cgatgccgat
    16081 gtgcagctcg acgccgaaga tcggacgcgt gctggggtga gcatcggttc ggcggcgggg
    16141 gatttggaag cgcgtctgcc tcttttggac gacttgttcg ccgcagccgc ccgtggcgac
    16201 gaagcggagg cgcagcgctg cgcgcgtcac gtcttgacca ctttcttggg acaggtggcg
    16261 ctgagcgtga acgggatgtt cggcctgcag ggtgatagca cggtcaccgc cgcagcgtgc
    16321 gcgtcgtcca ccgatgcagt gggctacgct tttcgccaga tccagtatgg cgaggccgac
    16381 ttgatgatcg caggcggcgc agaggcgccg atccagccct acatcttcta cagttacgcg
    16441 atgctcaacg tgctgtcgca gcacaacgat gagccggaac gcgcgtcgcg tcccttcgac
    16501 cgtacgcgca acggattcgt gatgggcgag ggcgcaggcg tgatcctgct ggaagagtgg
    16561 gagcatgccc ggcggcgcgg cgcgcatatc tacgcggagg tggccggcta cggcgcgtcg
    16621 gcggatgctt atcacatgac ggctcccagc ccgacgagcg aacagtcggc gcgggccatg
    16681 cgcctggcgt tgcacgaggc gggcgtacag cctgacgagg tggattacgt atgcgcgcac
    16741 ggtaccagta cacagttgaa cgacgtcaac gagaccaaga cgatcaagaa cgtgtttggt
    16801 gaagcggcgt gtcgcgtgcc ggtcagcgcg atcaagtcga tgctcgggca tacgcagggc
    16861 gcggccggga tcatgcaggc ggtgacgtgc gcgttagtga tggagcacgg tttcgtgccc
    16921 ccgacgatca actatcgcga gcctgatccc gaatgcgatc tggattacgt gccgaacgtg
    16981 ggccgggcaa ttcccattcg gacgatcctg caaaacacga tctcgttttt cgggcgcaac
    17041 aatgccgtcg tgtacaggaa gccagtggca tgagagtctt gctcatctac accaatcggg
    17101 agcagtcgcc ctatccggtc ctgccgctgg gactcgccta tgtggatgcc agcctgcgac
    17161 agcggggcat cgagacgcgc ctgctggacc tgtgctttgc aaaggatgcg gcgcaggtcg
    17221 cgcgcgatgc cgtgcagacg ttcaagccgg attgcatcgg cctgtccatc cgaaacatcg
    17281 acaacctgac ttacgatgcg cccgtgcgtt acctgcctgc gatacgccct atcgtcgatg
    17341 cctgccgtca ggcgttccat gcgcccatcg tggccggcgg gtcgggcttc tccatctttc
    17401 ctcaggccat actgcgcttc ttggacctgc aaatcgggat tgtcggcgaa ggcgaagagg
    17461 cgttcccgtt gtggctggcg tgcggtgacc cgcatcgtgt cccaggcgtt gtgacgaacc
    17521 gcgactcgcc aggcgacagg cagccaccgg ctcgtgtgcg cgatctcgat gctttgccca
    17581 ttcccacgcg cgatcgggca gtctcccgga cgtatgagct ggcaggcagc gcggttaacg
    17641 tccagaccaa gcggggttgc ccgtttcggt gcctgtactg caactacccc gtgatcgagg
    17701 ggcgagacgt gcggctgcgc tctccgcaga acgaaggcga agagttggag cgattggcgc
    17761 gcgccggcgt gaagaagatc catttcgtgg atgcggtgtt cacgtatccg ccggatcacg
    17821 ctgcggcgat ctgcgaggag atactgcggc gcaggctcga cctgcactgg gtgtgcggag
    17881 ccaacccgtc ctcgctgtcg cccgagctgg cctgcctgat gaggcgcgcc ggctgcagcg
    17941 gcgtcgagct gggcatcgac gcggcaaacg atgggatgct gcatcggttg cgcaaggggt
    18001 tcacgctgga cgacgtagcc gcctgcagtg agggctgctg ccaggccagg ttgccgttcc
    18061 tcttccatct gatcttcggc gggctaggcg agacggcgac aacgatcgag gagacgttga
    18121 cagccattgg caagatgcgc gccacggccg tgacgtattc attcggactg cgcatctttc
    18181 cggccaccgg cctggccgag gtgctccaac aggaaggggg ggcgttctcg gacgacgatc
    18241 tgctggaacc gcgtttctat ttgtcaccag agcttgcgcc gaagatgatg gagcgagtct
    18301 tgaatcacgc gcggcgggaa ccgacctggc tcgccccgcg tcaacgcgat tcggccatcg
    18361 ccaaggctat catgcgcctg ggcacacgcc tggggacgac agggcctgcc tggcgacacg
    18421 cagccgtcgg cgcgccgatc aagcgagcag tggccgtcgt caaagcggcg cggactaaaa
    18481 ggtcggtcgc tcgcgcttga ggtgccgcgc cgcccaggcg tcggatcgac gcgcaagcgg
    18541 ccaacgtagg agtcgatcgt ggacaaagtg gctatcgtga caggcgcatc gagtggaatt
    18601 ggcgaggcaa cggcgcgtcg cctggcgcgt gacggaatgt gtgtgacgct ggccgcgcgg
    18661 cgacgagaag aactcgaacg ggtggccgct gcaatcggcg acgagacgcg cgccctcgtc
    18721 gtcccgaccg acgtgcgcga tcgcgcggcc atccagcaca tggtccaggc caccctcgac
    18781 cggtggaggc gcgtggatgt gctcgtcaat aatgccgggt tgagttaccc gcggcgcgtg
    18841 atcgatctgg atcccgacca ggtgtgcgaa cagatcgacg tgaatctgat cgcggtgatc
    18901 gattgcgtgc aggcggtgct gccggccatg ctgcgccagt cgtcggggca catcatcaac
    18961 gtcgcttccc tcgccggtct gatcgggata ccgggcgcca gcatctattg cgcgaccaaa
    19021 tccgcgatcg tcggtttctc cgacgcgctg cggcgcgaag tgcaggacag cggcattcgc
    19081 gtgacggtgc taagcccagg ctttgtcgcc accaacttca gcccacgcct gcggcgcgcg
    19141 gccgagaagc tggccaacgg ccagcgcgtg cgcggcgtga tgtgttccga cgtcgtcgcc
    19201 gaacgcgttg ccagactgct ccggcatccg cgtcgccatg acatcctgcc gacgagctgg
    19261 cgccccctga ttgcattcct ccgagcattt ccagggctgg ccgatgtggt gctgagccgc
    19321 tactacgtcc gcatgcgtga gcgcatctga cctgtcgtgg ttcggcttac gcccagtggc
    19381 cggcacatcg cttgaggcgc gaactagact ggggtctagg tcggaactcg accgtgcgcc
    19441 attgtgcgcc gtgcggtttc ccaatactat acagatacaa gtgttgctag tagcaagcat
    19501 ccattcgcga tcaggcctgc cgagtcagcc ggtgaaggcg cgcggggcgg agggcactcg
    19561 tcgttggcgc cagttgagag atggggtgag catggaagcc gatctgaatt ggctggatag
    19621 acaaatcgcg ctcagggatg atgtcgctta ccgcaaagtg ggggacagcg tctatgtcta
    19681 cgcgctgcgc actggcgaaa cgagcaaact gaccggcgtc ggcgagttga tttgggggca
    19741 tttgacgcgc cagacaccgt tgcgcgaaat cgccgcttca ctcagcgacc agtttgccgt
    19801 gcctctcgcg caggtcgcgc aggatgtgtg caagttcgtc cgttcgctat acgaccgctc
    19861 gattgtcacg gccgatggcg aaactggcgg cgtgcgccgg tcattccgcg aagcgacgcg
    19921 ttggcagccg gagctcgtca tcaccgaggt taagcgacag cgggttccat ttcgtgttga
    19981 ttttgagacg acctacgcct gtaatctgga ttgcgtgtat tgctacacag ccaaagacaa
    20041 ccaccggctt ttgaatacat cagaagttca atcgattctt gatcaactgg ctgaagcggg
    20101 gacgctgttc ttgtgcttca cgggcggcga acccttcgcg cgtcgcgacc tggagcagct
    20161 catccggctc gcccacgagc gccggtttgc cattctggtt ttgacgaatg ccacgttgat
    20221 cacaccggcg tacgcagacc tgctggcgcg ctggtgtacg gtgcaggtca gcctgcacgg
    20281 cgcgcgcccg tccacgcacg atgcattcac cgctgtgaat ggatcgtttc gccgggcctg
    20341 ccggggtatc gagtatctgg ctcaagccgg cgcaaacgtt tacgtgatct tcaatgccac
    20401 gtatcacaac gtccgtgagg aagatgccct gcgccggctg tgcgagcaat ggcaggtggg
    20461 ctttgctctg aatgttcacc tgctgccaaa cgtcaacggc tcactcgcgc cactggatta
    20521 tcgtatttcg gatgactggg tgctggatct ggcgcggcag ggcaagctgg tgcgccggcg
    20581 aagcgtatgc acagccgcca ccactaaagc gcgcatctca ccattcggtg atgtttatcc
    20641 ctgcgagctg gtgcggcgtt cctttgggaa cctgcgtgag caggcgtttg aagatatctg
    20701 gcgcggggcc gagttggaag cattccgggc gtccgagttt ttctgcacgc ccgaagcctg
    20761 ccacgcttgc gcatggtcgg attactgccg tcgctgcccg gcgttggccg agatggaaga
    20821 cggcaatccg aatagccctt cgagcgaggc ctgccgcgtc gcgcgcttgt gcgccgaggc
    20881 gaagcaggaa acgtacgcgc ttgaacgaca gccagcgtga ggccactgcc tgtggcgcgc
    20941 actcatgagg gcatcaactg acattggtta aaacagagtt ggtatcaact tgttgcctct
    21001 gggcccgttg gtgtaccacg cccttgacgg tctcgtgtac agtcagcttg tgtgctgatt
    21061 ggcatctcgc gaatcggtcg tgagtatctg ccgagatgtg gatggcacca tgttccgaat
    21121 tctgaacttc caaggaggta taagcatgtt caagctcgtc gttggaaaga cgcgccgcac
    21181 cagtggcgcc gttgcgtgcg gctcaggctg ctaatcgagt cgctcttcga tacaccgtaa
    21241 ggcggggggg caaaaaccga gcgccgaaac cgggcgctcg tggttctcaa cggtgagacc
    21301 ataaaggacg gatttggcgc agcgtgccca gaggcgcccc gccacggttt caggggctat
    21361 gagccggacg tgttgatact cgatcaggag aatatggtgt gaacgatcac gattctggtg
    21421 ctgacgggaa agtcgggcag gcatggtctc acctgaagat cgcggacaac ctggcctatc
    21481 gcttcgtcgg tgatgaagtg ttctgctggc gaacaggcgc aaaggttgag aaccgattcg
    21541 ccattgtcgg cgggcttggc gtcgaagtgt tctcgtccgt cgaacggcgc gcggccttcg
    21601 acgcattcag agacgagata ctcgcccgct tcgatgtatc gcccgatgaa ttcgactccg
    21661 gtctggagga gtttctggtg tcggctgtgg cggcgggctt gttcaccgaa gaatccccga
    21721 atggcgcgac gagcctctcg ctcagatcac ccgtgtcgaa cggaggcgag ccggaagtgt
    21781 ccgaagccga tgtgttggat actatgtttg aaaaggccag cgcccaggtg ctgccgctca
    21841 aggtcttctt tgagttgacg catcactgca atcatcactg ccagcattgt tacttggggt
    21901 tcggcgtagg cgtgccgggc acgtcggcgc tctccacttc gcgccagcta gaactcctgg
    21961 acgagatggc gcaagccggc gtgttggagt tgattctgac tggcggcgag ccgacgctgc
    22021 accgtgcctt caccaagata ctgcagcgtg ctcatgaact gcacttcgcc gtggaactgc
    22081 tgactaacgg tacgcggctt gacgaagaac tagtcgaatt gctcgcctct gtttcgctgg
    22141 tcgaggtgcg ggtgcccata tacggcttag aagcctacca caaccgtttt gtgcggaacg
    22201 cacgttcctt caagaggacg tggaagggga ttgtggcgct ccatacccac ggcgttccgg
    22261 tcacggtcac cagcacactg atgagcgaca atgccgatca gttgttggag atcaagcgcc
    22321 ggctggactc actgggcatt cggtttcagg tcagcccgct cgtatatccc acgattcagc
    22381 gcaacgccgc tccaacgcaa taccgggcga ctcgcgaaca attcagggcg atgacgccac
    22441 aactgggcat cacgctgcgg cgcagccgat gcacggcggg ggtcagccga tttcgcatag
    22501 ggccggatgg ctcgttgcac ccatgcgaga tgttatcgac gatcgaattg ggcaacctgg
    22561 ctcaccattc tttcgagacg gttttgcgtt cccccgagcg ccaggagtgg atcgctcgct
    22621 tccgctctta tttggagacg aaagaccggg cgggctgtcg cgaatgccgt gtgcgatctt
    22681 actgcgtcga ttgcgtgggc ctgtcgtatc tggagagcac gacgtttgac ggccgttgtc
    22741 aagaagcctg ccaattggcg catgcgattg cctacatcga tcagcgtgat cagatggtag
    22801 ccgctctggc ctaattgcgc tgcgacacgg agccgcgcga actgtgtgaa gaggcgtcag
    22861 atggaagagt acgtcgagcg gttgtgttgc gacccagcca gcgtgaatct atcggaggca
    22921 aacaggctgc aacgcgatct gccgcagttg atacgcctgt tttatgtatt aagccgcgag
    22981 atgctaggcg ccctggacat caccattttg caaagctaca tcttaatggg tctgcaagtc
    23041 aacggcccga tgacgatgac cgaaatcagc gactatacca agttggcggc cagcacggtg
    23101 acgcgggtcg cggataagct tgtcaaagcc aacctgacag agcggatgtg cgacgaacgg
    23161 gatcggcgtg tcgtttgtct gaagctgtca gagagcggcg aagcgcgcag cacgcaaatt
    23221 cggacgttgc aaaacgtgtt tttccagggg ctgctgtcag gcttatcagc cctcgaacgc
    23281 gaatcgatcg tgtccggcct ggcgctccta acggggcgat tggcgcagtt cgccgaccag
    23341 cgacacgacg ctatcgacgt cggagaagac ctgctcgtca tggctgacac cgccttggcg
    23401 tgacggcccg gcgacattcg cgcgtgcgac tatcggttgg ccgtggcggg gatggaaggt
    23461 tcggaatgga taagcgacac gaggagaaaa acgcggttgg gcggctgagg cagcgagacg
    23521 agtttatcct ctcactcgct cgcgaggctt atcaggaaaa gcccttttac cgctggtttt
    23581 atgataacgc aagcgtggac gtagaacacg cggacgtgcg acgattgccc ttcatcacca
    23641 agaaggactt cttccactac gaggaagaga cggggcagcc ctattatctg ccgctgggct
    23701 atccagattc cagcttggaa tatctgcgta cctccggcac aacctgccag cctctggtga
    23761 taccctggct gaagtccgag tggtcgttcc tctacaacag cttgctgttg aacttcatcg
    23821 agcaaatcaa aggtcgcccg cgtttcctca acgccgtcaa tcccaatcaa ctggaagaga
    23881 tgcgagcctt ccagtcggtg ctggtgaatg tgccgcacga tcgcatggcc gacgcgccct
    23941 acgtcttaga cctcatcgcc aggcatcggc ccaatatctt tctcgataat tcgccgggcc
    24001 tgtggtttaa ctatctcctc gaccacggcc tagatccgtg ccgggccggc atccaaatgg
    24061 tctgtgcata tcccagcgcc gaactggccc agaaggtgga gtccttgggc ctgaagtacg
    24121 tcagcgcgtt tggctgtatg gaagccgggt tcttcggcta tgcgcacgcc ggcctgggcg
    24181 atgtgtacca cctgtccgat gcagagccga tccgttccgc cagcgactct ccaacgctgt
    24241 taggcggtat ccggctacag gtgttcagcc ggctgttccc ttgcgactgc tatcagggta
    24301 tggatctgca ggccatgttc ggccggcgcg atattcgagt gcgccgggtg gatggggaac
    24361 tgagcactgc cggtgcaggc gagttggtca tcacttgcgc gaattcggca ttccccttca
    24421 tcaagtactt aaacagcgat cgggtcgaga ttcatcctga ggctaagtgt gactgcggct
    24481 tcgtggggca gtcgatgcgg ttcgagtgcc gcgaccataa tgtccagatt ccttatctgg
    24541 gcggcggcat gaacgtcagc ctggaaggca tcgcgctcgg gctgaagggc tttcggcgcg
    24601 tcttcccgat tcatatcgca ccggacccga cggttcggat agtcgtcacg ttcgttgaag
    24661 acgaactcgc gacggcagcc tgcctggatc tcgacctggc tgagcgcatt atgacggcgg
    24721 caaccggcat caattcagag acgatgcgcc tcatctcatc gagaaggaac ttcttgccat
    24781 ttttcccggc cgttcgcatt ccggtcggga cgatacccct gcgagaccaa cattcgctca
    24841 aatctcgcct gtacctggat gcggtgtccg gatacgtgcc cgacgagtat cgacctgtgc
    24901 tggcgttgat cgaccagctt gtcgaccgcg ccgccatcat ccggtcggga agctggttct
    24961 tgcccgttga ggtttctcag ccaacgccgg cctgacggca atgccggcga gtgtacgcat
    25021 cgagcggttt cgcgattcct ccggccgggg agtcgatctc acatcctact agagaggagg
    25081 taagcacatg tacgccgagc ctgagctgat cacactcgat gttggcgagg aagcggaagc
    25141 tctgaaggag tgcgccgccg cttgctagtc cgtcactgac gagccagcga gcatagccag
    25201 ggctggattg tctcccctga ctgtcgagga gagtgttgcc tgtgcatgcg tcacggccct
    25261 tgcggccaac acggacgtca cgattgactg agcggtctcg gttagtggca ggcactcgtt
    25321 agcgactgcc ggacagtgcg acactctcca tcgggattac gaaggagtgt acggatgggc
    25381 agcaacggtc gtgcagaaca gcccacagcg aacgaattga aacgccggat cagtcgccac
    25441 gaggccgcgc gctcgcgaac cgtcggcgac gacacgttca tcatcagcct gcgtgaaggt
    25501 gcgaccatcc aacaccgtat ggatcgagtc ggaacatata tctggcagtt ggctgacggc
    25561 acccgaacag gcgaggagat ggcgcgcgcc gtggcgcggc actacgatgc cgacttggcc
    25621 gccgtcacgg acagtgtggc tcggtttgtg catgcactcc accagagcgg actcgtgaca
    25681 ctggagggtt gcacgacgcc cgctttctca cgtcagaatc gacgctccaa tttgcccgta
    25741 cctgcgcagg tcgcccgctt ttacggtgct gctgatgaga agtgcgtccc actggtggcc
    25801 actctgacgc tgacccatgc ctgcaatctg aagtgctatt actgctacga cgccggacag
    25861 cgcgggcgcg cgctggactt cagccactgg cgagacatcc tggatcaaat agccgaggcg
    25921 ggcacgctgt gcatccgcct gagcggcggc gaaccgacgg cgcaccccga cttcatgttg
    25981 attctcgaat acgccgtgca gcgtgggttt gtcacatcgg ttgtgacgaa cggcacgctg
    26041 atcaccccgg agctggccga gcgccttggc cggctggctc tcgaaagcgt cgccgtctcc
    26101 tttcacggcg gcagcccgga cgttcacgat cgtctggcag ggcggaacgg cgcatttgaa
    26161 cgcagctgcc acggcgtcgc actgcttcgg gagcatggcg tcaaggtcat tccggggatg
    26221 gtggtcactc gccgtaattt cgacgatgtg gacaatttcg cgcgattatc ggcgacctgg
    26281 ggactgtctc agcctatcct gtcgattgcc gtatacgccc gcaccaatgg cgcgaccgac
    26341 acgctgaacg atcgactgtc gctcgaccag attcgaaacc tgatgtcgcg aggttggtac
    26401 cggcccttcc ctaacttata ctgtggtgcg gctcggacca agtatgccat cacgcccaac
    26461 gggcgcgtga cgccgtgtca acgactgatg atcgaagtgg gcgacttgga gcgggagagt
    26521 ttcgccgaca tctggcgaca atcgcccgcg ctatgccaaa tgcgcgaaga acgctggttc
    26581 gcggatcccg accaatgccg gacgtgcccc gcttaccaga agtattgcag ccgctgcccg
    26641 gcgcaggcgt actacgaaca aggacaactc agggcgacct cgtcgttcga ctgccaactg
    26701 gtgcctgtat acctgcagtt gcagcaaacg acggaagtgt ccgcgccgac gcagcccgcc
    26761 tgcctgtgag caacggacag tggcgcgaga ggcagcaccg cgatgtgtga cgatcggaga
    26821 aagtctgatg aacgacggtg acgaattcat tctggcgctg gcgcgccagg cttaccaaga
    26881 gaaaccgttc taccgctggc tgtacgacaa tgctgctgtg gacgtggagc gggccggcgt
    26941 gcaagacatt ccgtttctca ccaaaagcga catcatccgc tacgaggaag aaaccggcca
    27001 accctactac ttgccggttg cctatcccga cacgcagatc gagtatcgac acacttctgg
    27061 cacaactaat cgccccatca cttttccctg gctcagagcc gaatggtttt tcctgtgtca
    27121 gagcatcttc ctcaatttta tcagccagat caaaggcagc ccccgatttc tcttttgtgt
    27181 aaatcctgac gaagaagaac gtgccaaggc ttttggctcg gtcctggtcg gtgtgtcgcg
    27241 cgacaggctc tccgacgcgc cgtacgtttt ggatctgatc gaacgcgaac gccccaatgt
    27301 catcgtggat aactcgccgg gcatctggtt caactacctg atcgaacacg gcctggagcc
    27361 gcgcgcggca ggcatccaga tggtgttgat gctgcccaac caggaactgg cgcacaagat
    27421 cgaggctatg ggcatccaat acggcagcgt gttcggatgc accgagggcg ggctgttcgg
    27481 ctactcgcac gccggcatcg gcgaggtgta tcacctgtcg gatgctgacc cggcgaccat
    27541 gctcaagttg gccatcagca gccagctcgg ccggttgggc ggcctcaagc tgcagatgct
    27601 gaccaggctg tttccgtgcg agtgttacca ggggcttgac cttcagcgta tgatcggccg
    27661 gcgcgacatc cgcatccagg cggagaatgg ccgcatggag aaaaccggct tcggcgagct
    27721 ggccattacc tgcgccaact cggtgttccc catcgtcaag cacttgaacg ccgacatggt
    27781 cgaaatttcg cccgccgccg agtgcgcctg tggcttctct ggacgctcta tgcgcttcca
    27841 ccaccgcgat cacgaagtcc aagtgcccta catcgggatc aatggcagca cttacaacct
    27901 gcaatggatt gcccaggaac tgaaaggttt caggcgcgtc tttcccgtct actacgcttt
    27961 gaatggcggc tttccacgca tcgtcgtgac gttcctggaa gatgatacgc cggacgtgcc
    28021 gcgtgtggac actggcatgg ccggacatat cctggctgcg tgctgtggca aaagcgaggc
    28081 ggaagtgcgc gagacacacg cttttgctaa acagcgattc ttcttccccg cgattcgcgt
    28141 gccgcccggc gccatcccca ttcgggacgg cgcgtccctc aagtcgcgcc tgtacctgga
    28201 taccgtgtcg gggcgcgcgc cggacgagta ccaaccgctg ttagccctag tggagcggtt
    28261 gatcgacccg tccccgatca ttcgcgcggg cagctggttt ttgccggtcg agcaaccggt
    28321 ggagggatga tgagcgacgg aatacgtacg cacaagttga ccaagcgcta cgccggcaac
    28381 gtcctggcgt tggccgagct cgatctggac gtggcggccg gcgagatttt tggactgctg
    28441 ggtcccaacg gcgcgggcaa gacgacggca gtgcgcatct tgacaaccat cttgcgacct
    28501 tcgtccggca cggccagtgt gggcggttac gacgtgcgca agcagcccgc ccaggtgcgc
    28561 cagcgcatcg gcgtcgtcgc gcagaatgcc tgcctggact gcatgttgac ggcgtatgac
    28621 aacctcgatt tctacgccaa gatccacggc ctgcgcggcg ccgagcgcgc acagcgccta
    28681 cgagaagcgt tccgctgggc caacctggac ggcaaggaac ggcacctggt tgattcgttc
    28741 tccggcggca tgaagcgcaa gctcgatctg gctcgcgtga tgatgctctc gcctcagttc
    28801 gtcttcctgg acgagccaac cgccggcctg gacccggcct ctcggcgtag tgtgtgggac
    28861 atggtcggcg aaatgaaagc gcgcggctgc acggtgatcc tcaccacgca ctatatggaa
    28921 gaagccgatc gcctgtgcga tcggctggcg attctgcatc gcggccgcaa agtggccgaa
    28981 ggtgcgccac acgagatcag gtcgcaggtg agcggcaaga gcagtctgga agtgcacgcg
    29041 cccaacctga ccgacgccat gctgattccc ctggtgcgcg ccggcgagat cgagaagtac
    29101 gtcattgtgg gcaattacca tcagcgtatc gcccggctgt atctcgacga tgcggtgggc
    29161 aagatgccct atgtgttgaa ctatctgcac gaccacggtg tgacggccaa cgccgtgacg
    29221 gtgcaggggc cgacgctgga agatgcgttc atcaacctga cgggtatgcg aatcgaggcc
    29281 tgatatgctc ggcgcattcg acgtgtggca gcgcgacttg aagttgatac gcaagttctg
    29341 ggtgtttttc gcggtgctcc tgctcgacat cgtcctgctg ctgctggcct tcggcccggc
    29401 tatgacgcgc ctgctgccgc gctttgcgta tccggcgggc tcaaccacgt tccagatcga
    29461 ttacttcaca ttcttcgtgc cgggcctggt tgtgctggtg gtcatgcagg ccagcctgta
    29521 catgggcgag atgagttaca tcgatcgcga catgggcgtg ctggagatct tgttcaccgc
    29581 acccatgtcg cgctggagca tctgcctggg caagctggcc agcgccacga ccgtcgccgc
    29641 cgtcatcgcc ggaacgctgt acgtcgtgtc atttgcgctg gggttccgac cgtacgatgt
    29701 cgggatcgga tcggccgtcg tgctgctaat tatcttgacg gccagctggg gattcgcggg
    29761 gtgcagcatc gccacggtga tgcagattcg cagcaccagt cgctggttgc tgctgttggg
    29821 cgcgatcctg tcgccgctca ccgccgccgc cgcgctgcag tatcccgtcc aggtcatgcc
    29881 cgaatggatt cggccgatcg ccggcgccaa ccctttgacg catgccgttg acatggttcg
    29941 cgcggcagct ctggaaggtc gttggccgga catcacgtcg ctgatcggct tgttcgggtt
    30001 cgcgatcgtc ggcacactgc tggcggtgac ctcgatgaag cactctcgat tgctgggccg
    30061 gtgaggcaca cgccacccga ctgaggtgaa cgcatcatgg aagtctggta tgtgtggcag
    30121 cgcgacgtgc gcctgatgcg ccgcatgatt aactcgttca cgatgacgat cctgggcacg
    30181 ctcgtcgcgc tggcgatctt cgcgccgacg atcgaccggc tggtagcggg catcgcgttt
    30241 ggaacggcca gcgtgcccta tctccagttc tacatcccgg gttcgctgat cctggccgca
    30301 ttcagcggga gcttttatgg cggcggatcg atccagcacg accggcgaga aggcgtgctt
    30361 caggtgatgg ccgcttccgc gctatcgcgg cgcgcattgg cgctgggcaa gatcgcgagc
    30421 gcagtgacgc gttccatgct catcgtgctg ggcgtgtttg cagcagtgac ggcggcgggt
    30481 ttccgcttca tccatccgct ggcgggggcg ctgggtctga tcgggatggc cgtgctgatc
    30541 agttggagct tcgcggcgat cagctcatgc ctcgccttcg tgattcgaca tcgctttggc
    30601 tatgacttgt tgatcaccgg tctgattgcg cccctatcgt acctggctgc gatcacttac
    30661 ccggaaaccg ttctgcccga cactcttcag gttttagcgc ggctcaaccc tttgacgtat
    30721 gccgccaacg gcgtgcgcga aataatcgtc tacggaacat ttccgtccgc gctgaatctg
    30781 ctcatgccgt tgggcttggc agggatatcc actgttctgg cggcgcggat ggcccggaac
    30841 ctgagcggcg agaagttgtg ggcgccttag tgcgtaggag ggatcgaaat gtctatcttg
    30901 ctcacaatgg cctggcgtgc cctatggctt cacaagctgc gcacagtgct gacgattatg
    30961 ggcgtgatgc tgggcgtggc cgtcatcctg gctgtacgca cggccaatcg cagcacgctc
    31021 cggtcgatcg agacgctctt cgatgaggca gccggaaaaa gcagcctcac tgtcgaaagc
    31081 gcggtgtcgt cccgctatgc gcagattggc ttctcggatg atacgctgcg gcaggcgcag
    31141 acagtcgccg gcgtggttca agccgtgccg cgtctgaccg cctcgaccgt gtttcagggc
    31201 aagaaaggcc cggtcgagct gtcggtgcag ggagttgatc cgaacgttga tcaggaagtg
    31261 cgcatctacc cgctggtgga cggttccttt ctgcccaccg ctggacggca acgcgcgatc
    31321 ctggtggtcg aggatttcgc cgagcgtgaa ggcatccgtg tcgggcgtga catcgagctg
    31381 ctcacgccga gcggcctgga gacctttcgt gtggccggca tcttggcgcg caagaacgcg
    31441 ggcgtgacga gcggcggacg cgtggtcttc gtctcgctgc gcatcgcgca ggaggcgttc
    31501 cagcgtggcc ggaagatcga tgctctggac gtggtcgtca aaccggacat cgcgcaatcc
    31561 ccggtggcgc ttcagcaatt caaagaacag ctgcagacgg cgctcggcga tgactacata
    31621 gtcttgtacc cggccgccaa gggcagcaac gtcgccgaag ccttgagcag cgtccaatat
    31681 agtctggagt tgttcggctt gttcgcgctg tttgccagcg cgctgctgat tcacaccacg
    31741 ttcacgatgg cgatcgccga acgcatgcgc gaagtcggaa tgctgcggat gttgggcacg
    31801 agccacgcgc aaatcgtcgg ccagctgctg ggcggcgcgc tgctattggg cgcgatcggc
    31861 gtggggttgg gcctgatcat ggggtttgtg atggccgtgc ccctgctggg tttcttcgcg
    31921 tcgctcaccg gttacgggct ggggacgttg tcaccgtcgc cgggcgacat cctgttgagc
    31981 ggcggcctgg gtctgatcgt gacgctgcta tcggcactgt ggccggtgat acgggccggt
    32041 cgactttcac cgcttgaagc ggtgcgggtg cgtgggcaga cgacggaagg ccgatcaatt
    32101 ggccggagct gggtggttgg gctggcgctg ctggcgatct ggggtgcttc gtttttcatt
    32161 ccgcagctac cgagcgcgcc gatgttcatc gtcttcttcg gcggcgccac actgctcttg
    32221 ccgtccgttg tgtatcaggc gagcaaacgg ttgcgtcgtc cggtcgcgac ggtgtacggc
    32281 tatgagggga gcctgggcgc cacaaatctg gaacgctccc gctggcgcac gagcacgact
    32341 gtcgctgtgc tgatgatcgg cgtcgcgatg ggtgtctcga ttggagcggt atcggatagc
    32401 ctgacgacca gtatccggtc gtgggtggac agtgccatgg gcgcggacct tttcatcagc
    32461 tcgtacagct cgatggaccc gctttggggc aggcgattgg ccgctatcga cggcgtggac
    32521 gtggtcagcc cgctgcgcat cctggacgcg cgagtcgaag acccgacgcg cgcaggccac
    32581 tatttcacgg cgcgtgtgca ggccatcgac ccggccacgc tgaagcagat cggcgaacag
    32641 agctacgtct ccgggcaggg cgctccggcc gacctgtggg cggcgttcga gcagggtggc
    32701 agcctgctgg tctcaacgca gtatgccgat cgctacgaca ttcgtcaggg agaccagctt
    32761 cgcctgcgca cggcgcgcgg catgcaggag tttccggtca ccgccgtgat cctggattac
    32821 aacagcatgc gtggcacagt gatgatttcg caagatgacc tgcggcggta cttcggccta
    32881 aacgatgcgg acgtttatct actcaagatc gcgacgggtg cggacgtcga ggccgtgcgc
    32941 cagcgcatcg acgcgcagta cggtcgctcc gggcagctca ccatccagtc gaactccgaa
    33001 gttcggcggt ctctcttaca aagcgtcggc tcaattatgc tggccttcaa tgccattgtc
    33061 gtgatcgcct tcatcatcgc cgcgtttggc atcgccaaca cactgacgat gaacgcgatc
    33121 gaacgcacgc gcgagatcgg catgctgcga gcggtgggca tgactcgctt tcaggtcggc
    33181 aagatggttc tggcggaggc gttggtgatg ggcgtgatcg ggggggtgtt tggcctcgcc
    33241 ttcggcggcc ttttttcgcg cgtcgtggtg tggtggatga gcagcggcat agggttccga
    33301 gtcgactatg tgtggcctgt cctggctgtg ttgatcagcg gcgcgctggt actgctcgtc
    33361 tctcagttgg cggccttcct acccgcgcgg cgcgcgtccg ggtatgccat cgtggaggcg
    33421 ctgcgatacg aataacttgg tcgcgagcgg ttcgccgcga gcgcacgtgt catgaaagga
    33481 gagttcgaca tgccaatcct ggaagcgcac gatgtcagca aacaatataa gctgagcgaa
    33541 caggtcacta tcagcgcatt gactcacgtc aacttccacg ccgaccgggg ggagttggtc
    33601 gcgattaggg gtccgtctgg ttcgggcaag agcaccctgc tgcatgtgct gggcggcatg
    33661 gaccgcccaa ccagcggtga gatcgccctg gacgggcagc ctctgtcccg gctcagcgat
    33721 cgcgagctga cactgttgcg ccggcgcaag gttggattca tctttcaatt tttcaacttg
    33781 attccgacct tgaataccga ggaaaacatc ctgctgccgc tgttgatcga cggcaagcgc
    33841 aagacgcagt gtctgcctcg cctggacgag attgtgagcc tcgtcgggct ggatcaccgt
    33901 cggcagcatc gacccgagca gctatcgggc ggcgaacaac aacgcgtggc gatcgcgcgg
    33961 gcgctgatcg ccgaaccgag cctcattctg gccgacgagc cgaccgggaa cctcgattcg
    34021 gcgatgggct tggagatcat cgagctgctc aaacgcctgg cgacgacgta tgcgcaggcg
    34081 atcgtggtgg tcacgcacga cccacgggtg gccgaacacg c
//
LOCUS       DBNHXB010000211         5530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_550885, whole genome shotgun sequence.
ACCESSION   DBNHXB010000211 DBNHXB010000000
VERSION     DBNHXB010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_550885"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..967)
                     /locus_tag="AC162G_08750"
     CDS             complement(248..967)
                     /locus_tag="AC162G_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HIR5075942.1"
                     /translation="MLQRFSDTDDAAIQYDELVAYVEASGRPRRIVQVDLEHHPKISQ
                     YTDIDSSPAGYGKNACGLVAAAAALGGEEWVSLVDQIAEAAGKNYGRYAGIQPSKYVS
                     ALLDVFGVENVTAKGRGSLGGLYRELEAGNIVIVDIKVHANTRVPSASGPNYAHFARV
                     LGLDVDKQEIYIENTLRGGPYWTLSLDSFLATWNRPETTASIILDPQNAENVTRWAVI
                     IDGALVPMESSEQMKSLESSL"
     gene            complement(1377..1583)
                     /locus_tag="AC162G_08755"
     CDS             complement(1377..1583)
                     /locus_tag="AC162G_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019554698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HIR5075943.1"
                     /translation="MSVDIMEGQWQQLRGKIREKWSKLTDDDLGQLGGGYDQLVGLLQ
                     AKYGYARDRAQHEVDKFLKAMKAR"
     gene            complement(1634..2296)
                     /locus_tag="AC162G_08760"
     CDS             complement(1634..2296)
                     /locus_tag="AC162G_08760"
                     /inference="COORDINATES: protein motif:HMM:NF017088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HIR5075944.1"
                     /translation="MQFTQGANVVTAQGQDVGNIDRVVIDPRSKEVTHIVVRKGLLST
                     QDKVVPIDLIAAATADRITLREDAADPQALPDFEEEYYVASGEDGAPPDPPLGSLPGL
                     YWYPPVAGGQIGYLFERSRQAPITQIQRNIPEGMVALKEGAKVISADDRYVGKIERVL
                     IDPQADRATHFLISKGLLLKETKIVPVQWVTNIGEDEVHLAVRSRLLNELRAHREPEP
                     VD"
     gene            complement(2386..3027)
                     /locus_tag="AC162G_08765"
     CDS             complement(2386..3027)
                     /locus_tag="AC162G_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR5075945.1"
                     /translation="MIRILLVDSSRLIGGMFTSVLDNEPDLQVVACATTVDQSLEHIH
                     ACDVALVSAALPDDGTYEFIRAARSANPQVKTLVVGLAGSKSAVIRHIEAGAAGYANR
                     DDSVDNLLSHIRAAHRGEALLAPDLAAALMARLVEWTTAPQKAQPRSIEAAGLTQRER
                     EVLRLIEQNLSNREIAKRLVIEVCTVKNHVHHILTKLDVNSRRDLVAYPAAEG"
     gene            complement(3199..3831)
                     /locus_tag="AC162G_08770"
     CDS             complement(3199..3831)
                     /locus_tag="AC162G_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5075946.1"
                     /translation="MTKTRVVLVDDHPIVRAGIRNLVEKAPDIEVVGEASNGAEALRL
                     AQELTPDVLLLDMEMPGLKGVEVAQQLNEAGSPVRILALSAYDDSQYILGLLAAGAAG
                     YLTKDEVPETIIEAVRGVSRGEQGWVSRRAAAKMAAWTHSVASGKGALTHREVEVLKW
                     VVDGKTNQEIGATLGISEKTVEKHLEAIFRKLGVASRVEAAVLAVKEGIV"
     gene            complement(3841..>5530)
                     /locus_tag="AC162G_08775"
     CDS             complement(3841..>5530)
                     /locus_tag="AC162G_08775"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5075947.1"
                     /translation="EASQALMAKTDLDARLESVTRLVVPRLADWCTIHLVEADESIRL
                     LTAAHRDPAKSARIMALVQSFPLDPDAPSGVPLVLRTGKPEIAVKVSDELLRAMAEDA
                     GHLRGLQELGIESYLIVPLVARGRTLGAMTLASALAGRRYAHRDLALVEELADRVALS
                     VDNALLYQETHLLNAELEQRAAKRTAQLQASNDRLRKQIAERRRAEEALQRSQRQYEE
                     LVDAVNGIVWEADAQTLEFHFVSRQAERMLGYPTTRWITEPTFWIDHIHPDDREWVID
                     YCTRAIAEQRLHEFEYRMIADDGREVWLRNMVTVVVRDDRPVKVRGVMTDITERKRAE
                     AELAEMQRRMMESREAERLHLAQELHDGPVQDLYGISFRLAGLRDAPRLRDGEPAEQL
                     AAGQQGLNQVIQTLRGITSELRPPTLAPFGLEKAIRSHAERFQEAHPELELHLDLMPD
                     QQKLPEPMRLALFRIYQQTLTNVIRHADARQATIRFQLGADRATLEIEDDGRGFDVPA
                     RWIDLVRQGHLGLAGAVERAEAVGGRLKVISAPGHGTRVQVSVPTQPRDNGPTR"
BASE COUNT          909 a         1794 c         1674 g         1153 t
ORIGIN      
        1 gtcgcttcgg ccgtcgcggt cgcttcggct gtcggcgttg ccgttgcctg acggagcggc
       61 gaaccggcct gttgcgccga aacgagcggc gcgagtgaca gcagcaccag caccaccgct
      121 ccgaccatga tcaatttgcg catgtgagac atcttcactt cactcctctc ttaactggac
      181 ttgggcaagt gtcatatctg gggttggagt cgaggcttta gccggtcttg accgcagata
      241 tgaccgccta gaggctggac tccagtgatt tcatttgctc tgaactttcc attggaacga
      301 gcgcgccatc gatgataacg gcccagcgcg tcacgttttc ggcattttga ggatcaagga
      361 tgatcgaggc ggtggtctca ggccgattcc aggttgctaa aaagctatcc agcgagagcg
      421 tccagtacgg gccgcccctc agcgtattct cgatgtaaat ctcttgctta tcgacatcca
      481 ggccgaggac gcgggcgaag tgggcgtagt ttggaccgct ggccgatgga actctcgtat
      541 tcgcgtgaac cttgatgtcg acgatgacga tattgccggc ctccagttct cgatacaacc
      601 cgccgagcga gcctcggccc ttcgccgtca cgttttcgac gccaaacacg tctaggagag
      661 ccgaaacata tttcgagggc tggattccag catagcgccc atagttcttg cccgccgcct
      721 cggcgatctg gtccacgagc gagacccatt cctctcctcc cagcgcggcc gcggccgcaa
      781 ccaagccaca tgcattcttt ccataaccgg cgggagatga atcgatgtcg gtgtactggg
      841 agattttggg gtgatgctcc aggtcgacct gcacgatccg gcgtggccgg ccagacgcct
      901 cgacgtaagc aaccagctcg tcatactgaa ttgcggcatc gtccgtatcg ctgaagcgtt
      961 gcagcacggg cgccgggaag aaggttgccg tcgagatcgg cagggtctga aagcctaata
     1021 cacccaggat gatgccgacc aggacgctga gcgccgggag ccggggttgc cgaaccctgg
     1081 tcggttgtga agatgacaac ttcatgtatc tcaagcccta tcctctctct tgacatctgt
     1141 ttccgtgcga tggcggggcg cgtcgagaat ctgccggcgt caagtcgggt tgctttcagt
     1201 gccggtctgg ctgcccttca actgtaacgc gcttccccgg cgggccagac ggtggccgtt
     1261 ctcctcctct cgccaggttg aagccgccgt tgcagcgacc tgaattggct caggcggacc
     1321 tctgcctcga cgcgccccat ccatctatcc ggctcggcgc cggcgccgcg ctgcccttag
     1381 cgggccttca tcgctttgag gaacttatcg acctcgtgct gggcccgatc cctggcgtag
     1441 ccgtacttcg cttgcagcag gcccacgagc tgatcgtacc cgcctccaag ctgcccgagg
     1501 tcgtcatcgg tgagcttgct ccacttctct cgtatcttgc cccgaagctg ctgccactgt
     1561 ccctccatga tgtccacact catcgccgtt ctcctttcga gtgatgctga tactgcatcg
     1621 gccgtattgg ccgtcaatcc acgggttcag gttcccggtg cgcacgcagc tcgttcagca
     1681 ggcgcgaccg gaccgccagg tgaacttcat cttcaccgat gttcgtcacc cactgaaccg
     1741 ggacgatctt cgtctctttg aggagcagtc ccttgctgat cagaaagtgc gtggcgcggt
     1801 ccgcctgcgg atcgatgagc acgcgctcga tcttacccac gtaccggtcg tcggcgctga
     1861 tcactttagc gccttccttc aaagcgacca tcccttccgg gatgttccgc tggatctggg
     1921 tgatgggcgc ttgtcgagac cgctcgaaaa gatagccgat ctggccgcct gcgacaggcg
     1981 gataccagta taggccgggc aggctgccca gtggagggtc aggcggtgcg ccgtcttcgc
     2041 ccgacgcgac gtagtattcc tcctcgaaat cgggtagggc ttgcggatca gccgcatcct
     2101 ctcgcaacgt gatgcgatcc gcggtcgccg ccgcgatcag atcgatgggc acgaccttat
     2161 cttgagtgga gagcaatcct tttcgcacta cgatgtgcgt gacctctttc gatcggggat
     2221 cgatgaccac ccgatcgatg ttgccgacgt cctgtccttg tgccgtgacg acattggctc
     2281 cttgcgtaaa ctgcatggcg gcgctcctat ctctctggtg attattcaca tctcgctcct
     2341 agcgccgccg gggacggcgg cttgagcgtt gtgtgtaatc accggctatc cttccgcagc
     2401 gggatacgcg acgaggtccc gccggctgtt cacgtccagt ttcgtcaaga tgtggtggac
     2461 gtgattcttg actgtgcaga cttcgatcac cagccgtttg gcgatctcgc gattgctgag
     2521 attctgctcg atcaatcgaa gcacctctcg ctcgcgctgg gtcagtccgg ccgcctcgat
     2581 cgacctgggc tgagcttttt gtggcgccgt agtccactcg accaggcgcg ccatcagcgc
     2641 ggcggccagg tcgggtgcga gcagcgcctc accgcgatga gcggcgcgga tgtggctcaa
     2701 caagttgtcg accgaatcgt cgcgattcgc gtatccggcc gcgccggcct cgatgtgacg
     2761 aatcaccgcc gacttcgacc cggccagacc gacgaccagg gtcttgacct gcggattggc
     2821 actccgggcg gcccggatga actcgtacgt gccatcgtcg ggcagggcgg cggacacgag
     2881 cgccacgtca caggcgtgga tgtgctcgag cgactggtcg accgtcgtcg cgcaggcgac
     2941 gacttgcagg tccggctcgt tgtccagcac gctcgtgaac atgccgccga tcaagcggct
     3001 ggaatcgacg agaaggatgc gaatcatagt cggctcctcc actgttcgtc ggcctgctcg
     3061 cacatcacac tgctgtcaga acgagtgatc ccccggtgca cactatatgc gcactcgagg
     3121 gatcaatcaa tgggggatgt gccttttttg aggtagggat ttccctaccc tggcacgagg
     3181 gggattacat gagcgtcgtc aaacgatacc ctctttgaca gccagcacgg ccgcttccac
     3241 gcgcgaagcg acgcccagct ttctgaaaat cgcctccagg tgtttctcga cggttttttc
     3301 gctgatgcct agcgtcgcgc cgatctcctg attcgttttt ccgtcgacga cccatttcaa
     3361 gacttccact tctcgatggg ttagcgcacc tttgccggat gccacgctgt gcgtccaggc
     3421 ggccatcttc gcggcagccc ggcggctgac ccagccttgt tcgccgcgcg acacaccgcg
     3481 caccgcttcg atgatggtct ccggcacttc gtctttcgtc aggtagccgg ccgcccccgc
     3541 cgccagcagg cccagaatgt actgactgtc gtcgtaggcg ctcagcgcca ggatgcgcac
     3601 gggcgagccg gcctcattca gttgctgcgc cacctcgaca cctttgaggc cgggcatttc
     3661 catgtcgagc agcagcacat cgggcgtgag ttcctgcgcc aggcgcagcg cttcagcgcc
     3721 attgctggcc tcgcccacga cctcgatgtc gggcgctttc tcgaccagat tgcgaatgcc
     3781 ggcgcgcacg atgggatggt catccaccag cacaacacgt gttttcgtca tcgtccttcg
     3841 tcaccttgtc gggccattgt ctcgtggctg ggtcggcacg ctcacctgaa cccgcgtccc
     3901 gtggccgggc gcggagatga ccttcagtcg cccgcccacc gcctcggcgc gctcgacggc
     3961 gccggccagt cccaggtgtc cctgacgcac cagatcgatc cagcgcgccg gtacgtcgaa
     4021 tccgcgcccg tcgtcttcga tttcgagcgt tgcccgatcg gctcccaatt ggaagcggat
     4081 tgtggcctgg cgcgcgtcgg cgtggcgtat cacgttcgtg agcgtctgct ggtagatgcg
     4141 gaacagggcg agccgcatcg gctcgggcag tttctgctga tcgggcatca agtcaaggtg
     4201 caattccaat tcagggtggg cttcctggaa gcgctcggcg tgtgagcgga tcgccttctc
     4261 caggccgaac ggcgcgagcg tcggcggccg cagctcactc gtaatgccgc gcagcgtctg
     4321 aatcacctgg ttcagccctt gctgtccggc ggccaactgt tcggcaggct cgccgtcgcg
     4381 cagacgcgga gcgtcccgca acccggcgag ccgaaacgag atgccgtaca gatcctgcac
     4441 tggcccgtcg tgcagctcct gggccaggtg cagccgttcg gcctcacggc tctccatcat
     4501 ccggcgctgc atctcggcca gttcagcctc ggcgcgcttg cgctcggtga tgtccgtcat
     4561 cacgccgcgc actttcacgg ggcgatcatc cctgacgacg acggtgacca tgtttctcag
     4621 ccaaacctct cgaccgtcgt cggcgatcat gcgatattca aactcgtgca gccgctgttc
     4681 tgcgatcgct cgggtacagt agtcgatcac ccattcgcga tcgtccgggt ggatgtggtc
     4741 gatccagaaa gtcggttcgg tgatccagcg tgtggtcggg tagccgagca ttcgctcggc
     4801 ctgccggctc acgaagtgaa actcgagggt ctgcgcatcc gcttcccaca caatcccgtt
     4861 gaccgcgtcg acgagttctt cgtactgccg ctgcgagcgc tgcagcgcct cctccgcccg
     4921 ccggcgctcg gcgatctgct tgcgcagccg gtcattgctt gcctggagct gcgcggtgcg
     4981 cttggcggcc cgttgctcca gttcggcgtt cagcaggtga gtttcctggt acagcagcgc
     5041 gttatcgacg gacaacgcga cgcgatcggc gagttcttcg accagcgcca gatccctgtg
     5101 tgcgtagcgg cggcccgcaa gggccgaggc cagcgtcatc gcgccgagcg ttcgcccccg
     5161 tgcgacgagc ggcacgatca ggtacgactc gatgccaagc tcctgcaagc cgcgcagatg
     5221 gccggcgtct tcggccattg cacgtagcag ctcatcggaa accttcaccg cgatttccgg
     5281 cttgccggtg cgcagcacga gcggcacgcc ggagggcgcg tccggatcga gcgggaagct
     5341 ttgcacaagc gccatgatcc tggccgactt agccggatcg cggtgtgccg cggtaagcag
     5401 acggatggat tcgtctgcct cgaccagatg gatggtgcac caatcggcca gccggggcac
     5461 gaccagacgc gtcacgctct cgagccgcgc atccagatcg gtcttggcca tgagcgcctg
     5521 gctggcttcg
//
LOCUS       DBNHXB010000212         4961 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2397197, whole genome shotgun sequence.
ACCESSION   DBNHXB010000212 DBNHXB010000000
VERSION     DBNHXB010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2397197"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="AC162G_08780"
     CDS             <1..737
                     /locus_tag="AC162G_08780"
                     /inference="COORDINATES: protein motif:HMM:NF020260.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair MutL family protein"
                     /protein_id="HIR5075948.1"
                     /translation="AEVRFYQERAIYNAVTQAVGEALRDFPLLQPVPTDWSFADAAGT
                     EPVASALREPRADYAIGPWRAVAQIHNTYILAQSAEGLVIVDQHAAQEQVFYERLTIS
                     NPQSPITVSRQIQLMPQEAGLLLAHLEDYQSLGIDIEPFGTNTFRVTGMPGFIQMDAR
                     ELLTALVQEHERYRTLEGDALRDKLASKAACLSAIKAGDVLDAAQQQALLDELLQVYS
                     PATCPHGRPVFVTLRLEELERRMGRR"
     gene            complement(711..1028)
                     /locus_tag="AC162G_08785"
     CDS             complement(711..1028)
                     /locus_tag="AC162G_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HIR5075949.1"
                     /translation="MKTLPPVHPGEVLLEDFMKPLGLSQYRLAKDIGVPALRISQIVR
                     GKRSITADTAMRLARYFGTSAVVWLRLQARYDLEVAEARLAKRINREVKVLKQLTPPH
                     PSL"
     gene            complement(1039..1323)
                     /locus_tag="AC162G_08790"
     CDS             complement(1039..1323)
                     /locus_tag="AC162G_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR5075950.1"
                     /translation="MIESFADVETEKVFRGVVSRKLPPDIQKTARRKLLYLDDAEDLR
                     DLRAPPGNRLEELRGDRAGQYSIRINDQWRICFKWVNGKARDVEIVDYHR"
     gene            complement(1618..2082)
                     /locus_tag="AC162G_08795"
     CDS             complement(1618..2082)
                     /locus_tag="AC162G_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075951.1"
                     /translation="MLTVNGILTNPIAPQAGIAHALRQYQMARLRGRLGQWWAALTGR
                     CRSLLQLDATTRENGVGARHYAGIQAVPIRQIRGTEERARDFDVEFNPLTDHTQGRWL
                     SVFTALQRGAALPPVELTRLGETYFVRDGHHRISAARALGQEYIEAIVTVWQ"
     gene            2263..>4961
                     /locus_tag="AC162G_08800"
     CDS             2263..>4961
                     /locus_tag="AC162G_08800"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR5075952.1"
                     /translation="MSRLTLYLFGPPRLELDGAPVAIDRRKALALLVYLAVTRQSHSR
                     ESLAALFWPDYDQSRAYAYLRRTLWEINQALGEGWVMADRETVGLNPAVDPSSDSGQA
                     LWLDVARFRNLIAERRKHGQGCAAGLSLLTEAVELYQNHFLAGFNLKDAPDFDEWVFF
                     EAEGLRRDLAGALETLARCLSEQGDTEASISYARRWLALDTLNEAAHRQLMGLYEQAG
                     QHNAALRQYQECTRILHEELGAIPQAETTALYERIKADGGRTKEEGKKPASSLIPHPP
                     SLRHNLPPQPTPFIGRADELGQIAALLSNPDCRLLTLIGPGGAGKTRVALQAAGAQLA
                     AFSDGALFAPLASVGDPAFVVSAIADAVGFSFCSPSTAADQPTQKRQLLNFLSQKHML
                     LALDNFEHLLPAASLLTDILQAAPRVKIVATSRERLNLQDEWVLTIAGMRVPASDAQE
                     DAQAYSSVQLFVQNARKAVVGFMLTEKERPSVVRICQLVEGLPLGLELAAAWVKLLSC
                     RDIAEEIERSLDFLATTLRDVPERHQSLRAVFESSWNLLGAPEQDAFRRLSAFRGGFQ
                     REAALAVAGATLPVLAALVDKSLLYRRDADRYELHEVLRQYAAEKLDAAPDDKAQTLA
                     RHSAAYAGLVYARLAPLYGRGQKQALEQIAAEIENIQAGWRWAVTQRDAVRAEEYLEG
                     LFRFYDARSRFQEAEELSRQALAAFPHGDDSSEDETAIAGQLLGWHSHFCYQTGRLAL
                     ASDHMRQALACVRRLGPRHRHALAFVNLLAIYWQGIDTTIDAAEARRMARDNLAYYRA
                     QHNCWGIAQMLIILAWEAEWQGSRSRAKRLYRQALKIQREIGDDLGAARALNWLGELQ
                     HHIGEYAEARQSYQASLDLSREFDDRWAMSQSLDYSGYVARRMG"
BASE COUNT          920 a         1556 c         1580 g          905 t
ORIGIN      
        1 aggccgaagt gcgcttttac caggagcgcg cgatctataa cgccgtcacg caggccgttg
       61 gagaggcgct gcgcgatttc ccactgctcc agcctgtccc gaccgactgg tcgttcgccg
      121 atgcagcagg tactgagccg gtcgcctcgg ccttgcgtga accgcgtgcg gactatgcca
      181 ttgggccgtg gcgggccgtc gcccagattc acaacaccta catcctggcc cagagcgcag
      241 aaggcctggt gatcgtggat caacacgccg cgcaggagca ggtcttctac gagcgactga
      301 caatctccaa tccccaatcc ccaattaccg ttagtcgcca gattcaactg atgccacaag
      361 aggccgggct gctgctggcg cacctggagg actatcagtc gctgggtatc gacatcgaac
      421 ccttcggcac gaacacgttt cgggtgacgg ggatgccggg cttcatccag atggatgcgc
      481 gagaactcct cacggcgctg gtgcaggagc acgagcgtta tcggacactc gagggagacg
      541 cgctgcgcga caaactcgcg tcgaaggctg cctgcctcag cgcgatcaag gccggcgacg
      601 ttttggatgc cgctcagcag caagcgctcc tggacgaatt gcttcaggtg tattccccgg
      661 caacctgccc gcacggtcgc ccggtgttcg tgacgctgag gctggaggag ttagagcgaa
      721 ggatggggag gcgttagctg cttcaacact ttgacttcgc ggttgatgcg cttcgccaac
      781 cgggcctcgg ccacttcaag atcgtagcgc gcctgcaggc gcagccacac cacggcgctg
      841 gtgccgaaat agcgcgccag tcgcatggcc gtgtcagccg taatcgagcg cttgccacgc
      901 acaatctggc tgatccgcaa tgccggaacg ccgatgtcct tcgccaatcg atactggctc
      961 aagcccaacg gcttcatgaa atcttccagc aagacttctc cgggatgcac gggtgggagt
     1021 gtcttcatgg atgccctctt acctgtgata atcgacaatc tccacgtctc tggccttgcc
     1081 attgacccac ttgaaacaga tacgccattg gtcgttaatg cggatactat attggccggc
     1141 gcggtcgcct cgaagctcct caagccgatt accgggcggc gcccgcaagt ctcgtaagtc
     1201 ctctgcgtcg tccagataca gcagcttgcg ccgagcagtt ttttgaatat ccggcggcaa
     1261 tttcctcgat acgacacctc tgaagacctt ctcggtttcg acgtcggcga acgattcgat
     1321 cacgcaactg ataatatcac ataacgatat tatcgtcaag cggtcagacc tgggggagtg
     1381 caatacagaa ctgcggtgat tccaggtgct cgtcggcggt ctgtctcaca gggaggcttc
     1441 gcactgaccg tcgaccccgg atcggtcggg tcacaggccc tgcccggagc aagaaggtcg
     1501 gggaggcgtt gagcgggcag gctgaacact tgtgccaggg tgtttgcgcg ctcgatatga
     1561 aacagatcgg gcacggccag gaaagccggc gagtgcgcgc gaagagggac gacccagcta
     1621 ctgccacacg gtcacgatcg cttcaatgta ctcctgtccc agcgcgcgcg ctgccgagat
     1681 gcgatgatgc ccgtcgcgca cgaagtaggt ttcgcccagg cgcgtcagct cgaccggagg
     1741 cagggctgcg cctcgctgca aggcggtgaa cacgctcaac cagcgtccct gggtgtgatc
     1801 cgtgagcggg ttgaactcca cgtcgaagtc gcgtgcgcgc tcctccgttc cccgaatctg
     1861 ccggatagga actgcctgaa tgcccgcgta gtgtcgcgcg cccacgccgt tctctcgcgt
     1921 cgtcgcatcc agttggagca ggctgcggca gcgcccggtc agggctgccc accactggcc
     1981 gaggcgcccg cgcaggcgcg ccatttgata ctggcgcaaa gcgtgtgcga tgccggcctg
     2041 tggagcaatc gggttcgtca gaattccatt cactgtaagc atggctggga tcctcctccc
     2101 tgaccactct tcttcagtgt cgcttccaga atagcctgcg cccgttgcaa gcccgttgcg
     2161 caagccgttt cgagcggcgt ttcggaggaa aattcgggcg actctgtctg cgagagaacc
     2221 catttggcta tataatctgg tcgagatgga ggcttccatc cggtgtcccg cctgaccctg
     2281 tatctgttcg gcccgcctcg cctcgaatta gacggcgcgc cggtagcaat tgaccggcgg
     2341 aaagcgctgg ctctactggt ttacctggcc gtgaccaggc agagccacag ccgcgagtct
     2401 ctggcggcgc tgttctggcc ggattacgac cagagccgcg cctacgccta cttgcgccgt
     2461 acgctgtggg aaatcaacca ggcactggga gaaggctggg tgatggccga ccgcgaaacg
     2521 gtgggcctca acccggcggt cgatccctcg tctgactcgg gacaggccct gtggctggac
     2581 gtggctcgat ttcggaatct gatcgccgag cgccgcaagc atggccaggg gtgcgcagcc
     2641 ggcctgtctc tgctcactga ggcggtcgag ctgtatcaga atcactttct ggccgggttc
     2701 aacctgaagg acgcgcccga ctttgacgag tgggtgtttt tcgaagccga gggcttgcgc
     2761 cgcgatctgg ccggcgcgtt ggaaacgctt gctcgctgtc tctccgaaca aggcgatacg
     2821 gaagcctcga tttcgtatgc gcggcgctgg ctggcgcttg acaccttgaa cgaggccgcc
     2881 catcgccaac tcatggggct ttacgagcag gccggccagc acaacgctgc tctgcgccag
     2941 taccaggagt gcacccggat attgcacgaa gagctgggcg cgatccctca agccgagacg
     3001 acggcgctgt acgaaaggat aaaggcagat ggcggaagaa cgaaggagga aggcaagaag
     3061 ccggcttcat ccctcatccc tcatcctcca tccttacgcc acaatcttcc gccgcagcca
     3121 acacccttca tcgggcgtgc cgacgagctc ggtcaaattg ccgcgctgct gtccaatccg
     3181 gactgccgcc tgctcacgct gatcgggccg ggcggcgccg ggaagacccg ggtggccctg
     3241 caggcagccg gagcgcagtt ggccgcattc tccgacggcg cgttgttcgc gccgctcgca
     3301 tcggttggcg atccggcgtt cgtcgtctcc gccattgccg acgcggtggg gttttcgttc
     3361 tgcagtccgt caaccgccgc cgaccagccc acgcagaagc gtcaactact caacttcctg
     3421 agccagaagc acatgctgct ggcgctggac aacttcgagc accttctgcc cgcggcgagc
     3481 ctgctgacag acatcttgca ggccgcgcca cgggtgaaaa tcgtggcgac ttctcgcgaa
     3541 cgactcaacc tgcaggacga gtgggtcttg accatcgcgg gcatgcgcgt gcccgcgagc
     3601 gacgcccaag aggatgcaca agcgtacagc tcggtgcaac tgttcgtgca aaacgcgcgc
     3661 aaagccgtgg tcgggttcat gctgacggag aaagagcgcc cctccgtcgt gcgcatctgc
     3721 caactggtcg agggcctgcc gctcgggctt gagctggcgg cggcgtgggt caagctgctc
     3781 tcttgccgcg acatcgccga ggagatcgag cgcagcctgg atttcctggc gacgacgctg
     3841 cgagatgtgc cggagcggca tcagagcctg cgggcggtgt tcgagtcttc gtggaacctg
     3901 ctcggcgcgc cggagcaaga cgccttccgg cgtctttcgg cgttcagggg aggatttcag
     3961 cgcgaagccg cgctcgccgt cgcgggcgca acgctgccgg tgctcgcggc gctggtcgat
     4021 aagtcgctgt tgtaccgccg ggatgcggat cgctacgagc tgcacgaggt gctgcggcag
     4081 tacgccgccg aaaaactcga cgccgcgccg gacgacaaag cgcaaacgct ggcccgacac
     4141 agcgccgcct acgccggcct ggtatacgcc cggctcgcgc cgctgtacgg gcgtggtcag
     4201 aaacaggcgc tcgaacagat cgccgcagag atcgagaaca tccaggcggg ctggcgctgg
     4261 gcggtgacgc agcgcgacgc ggtgcgcgca gaagagtacc tggaaggact gtttcgattc
     4321 tacgatgcgc gcagccggtt tcaagaagcc gaggagcttt ccaggcaggc attggctgct
     4381 tttccacacg gggacgactc ttcagaggac gaaacagcaa tcgccggcca actgttggga
     4441 tggcacagcc acttctgcta ccagacgggc cggctcgcgc tggcaagtga tcacatgcgc
     4501 caggccctgg cctgtgtgcg ccgcctgggg ccgcgtcaca ggcacgcgct ggcgttcgtc
     4561 aatctactgg ccatttactg gcaagggata gacacgacca tcgatgcggc cgaagccagg
     4621 cgcatggcgc gggacaacct tgcgtactat cgcgcgcagc acaactgctg ggggatcgcc
     4681 cagatgctca tcatcctggc ctgggaagca gagtggcagg gcagtcgatc ccgggccaaa
     4741 cgcctgtatc gccaggccct gaagattcag cgggaaatag gcgacgacct gggcgcggcg
     4801 cgcgcgctca actggctggg cgagctgcag caccatatcg gagagtacgc ggaagcgcgg
     4861 cagtcttatc aagccagcct ggacctgtcg cgtgaattcg acgatcgctg ggctatgtcg
     4921 cagagcctgg actattcggg gtacgtggcc cgccggatgg g
//
LOCUS       DBNHXB010000213         4462 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2397252, whole genome shotgun sequence.
ACCESSION   DBNHXB010000213 DBNHXB010000000
VERSION     DBNHXB010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2397252"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="AC162G_08805"
     CDS             complement(<1..322)
                     /locus_tag="AC162G_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075953.1"
                     /translation="MKATLRVLAALAALGLIVAACSTAAPTPIIQTVEVPREVVVTQE
                     VERVVEVTPTPIGGELVLYACLFDPDKLEVLFQTFKAQYGVDVTCLDMSSGEALERIR
                     AEQDN"
     gene            complement(527..1423)
                     /locus_tag="AC162G_08810"
     CDS             complement(527..1423)
                     /locus_tag="AC162G_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR5075954.1"
                     /translation="MPIQIATAPVSWGILEFVGQSSGQPWQSVLDEMVAAGYEGTELG
                     PYGFLPTDANALKTALSARGLRLLSAFVPVRLADRSAHEAGLMTARTIGALLAACGCK
                     HIVLSDDNAADPRRLSRAGRISPDDGLEESGWRALADGVSLVARQMHDELGLETVFHH
                     HCAGFVETPDEVAHLLDLSDPSLVGLCLDTGHYAYGGGDPVECIRQHGARVRYVHLKD
                     VDPSVMAQARREGWDYFRAVAAGLFCELGRGCIDFPGVIAALNALGYAGWAVVEQDLA
                     GMAGTPLDSARRNREYLMGLGL"
     gene            complement(1546..3360)
                     /locus_tag="AC162G_08815"
     CDS             complement(1546..3360)
                     /locus_tag="AC162G_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014328257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR5075955.1"
                     /translation="MQLTGAQIIAHSLVKAGVPYALGIPGHGCWCLTDAFLDVRDQLT
                     TLMVMHEQSAVHVADGYFRATGRPLAAFTSIGPGSTNTVIGMATAFVDSSAVLLMTGS
                     THTYMRGHAVMQELDRAQASNNLRIFEPVSKRQYLAVSAQGLPHIMHRAFNAMLSGRP
                     GPVHLDLPMDVQAEAADVDLPPDDQPGGFDRRVPAARPRPDADLTQRAARLLYGARRP
                     VIVAGGGVILSNAAKELVALAEYLGAPIVTTWQGKGAIPEDHPLNAWGVGTQASHCGN
                     QLASTADVLLAAGCRFVDWTSSSFRQGVTYSIPPTKLIHLDIDPGEIGKNYPAEIGLV
                     ADAKAGLADLLDAARQVGKAKDYSSSSYFAEIQAKKADWDRVLGANRDSDATPVTQAR
                     VMKELRAALDRDAIVTSGAGHVQGVVRQAFPVYEPRTHITSGGFSTMGFTVPAAIGAQ
                     LAHPGRQVVGCAGDGDFMQTMQEMAVAAMVDLPVLFVVLNNSGFISIKGGQMHNFGRT
                     TVVDFQKKDGGLYTPNLCEAARAFGLYSERIHAPGEVQPAVKRALATKGPALIEVMVD
                     REVARGVTPTGWWDMPVPEYLPDRRAVYEKERGEEAIG"
     gene            complement(3446..>4462)
                     /locus_tag="AC162G_08820"
     CDS             complement(3446..>4462)
                     /locus_tag="AC162G_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR5075956.1"
                     /translation="RENPVGPASPCRPSPAEAILKIGLFTDVLQDLSFAEALDWVAGQ
                     GIEAVEIGTGNFSNAPHCDVDRLLNDADARARFKDAIESRGLTLSALNCNGNLLDPHP
                     ERGKKSQDTFFKTVEAANRLGLDTIVTMSGCPGDPTGGEYPNWVTCTWQREFAELGEQ
                     QWAEVVEPFWKKAGRFAADRGVKIAIEMHPGQSVYNTRSLLRLRDVAGPGIGANLDPS
                     HLFWQGMDPLVVVRALGRNGVFHVHAKDTRIDPQEMALNGGLDTRPTSMVGERSWAFR
                     TVGYGHGAAWWRDFVSTLRLMGYDGVLSIEHEDRLMGSREGIVKSVEFLRPIVLKTTA
                     EEWI"
BASE COUNT          753 a         1501 c         1407 g          801 t
ORIGIN      
        1 ggttgtcctg ctcggcgcga atgcgctcga gcgcctcgcc gctgctcata tccaggcagg
       61 tcacgtcgac gccgtattgc gccttgaagg tctggaacag cacctccaac ttatcggggt
      121 cgaacaggca ggcgtataaa accaactcgc cgccgatcgg cgtgggcgtc acctcgacga
      181 cgcgctcgac ttcctgggtg acgacgactt ctctgggcac ttcaacggtt tgaatgatcg
      241 gagtaggcgc ggcagtgctg caggcggcca cgatcaggcc aagcgccgcc agcgccgcta
      301 ggacgcgcag tgtcgccttc atggtcaatt cttctcctct ctcggttggt tcaggtggtg
      361 ttaccggtgc cggcctcgcg cgggcgaggc tacttcgata cagacgagcg aatacgggtg
      421 agattcacct cctttccagg caggaatgca aaccaggacc acgttgtcac gttttacgct
      481 ttacgctagg ttccttgagt tccttgaatt ccctgagttc ccccatttat agcccgagtc
      541 ccattaagta ttcgcggttt cgccgcgcgc tgtcgagcgg ggttcccgcc atgccggcca
      601 ggtcctgctc gacgactgcc catccggcat agccaagcgc gttcagcgcc gctatgacgc
      661 cagggaaatc gatgcagccg cgtccgagtt cgcagaacag accggcggcg acggcgcgaa
      721 aatagtccca gccttcgcga cgcgcctgcg ccatgacgct cggatcgacg tctttcaaat
      781 gaacgtaccg cacgcgcgca ccgtgctggc gaatgcattc caccggatcg ccgccgccgt
      841 aagcgtagtg cccggtatcg agacacagcc ccacgagcga cggatcgctc aggtcgagca
      901 ggtgtgccac ctcgtcaggt gtctcgacga atcctgcgca gtggtggtga aagaccgtct
      961 ccaaccccag ctcgtcatgc atctgccggg cgacgaggct aacgccatcg gccagcgcac
     1021 gccagcctga ctcctccagc ccatcgtcgg gactgattcg cccggcccga ctcaaccggc
     1081 gcgggtcggc tgcgttatcg tcggatagga cgatgtgctt gcagccgcac gccgccagca
     1141 gcgcgccgat cgtgcgcgcg gtcatgagtc ctgcttcgtg cgcgcttcga tccgccagcc
     1201 gcaccgggac aaaggccgac agcagccgca gcccgcgcgc ggacaatgcg gtcttgagcg
     1261 cattcgcgtc cgtcggcagg aagccatacg ggccaagctc ggtgccctcg tatcccgccg
     1321 cgaccatttc gtcgaggaca gattgccatg gctgtcccga gctttgcccc acaaattcga
     1381 gaatacccca ggaaacgggt gcagttgcaa tctggatagg catacacaga tcaggtcaaa
     1441 gttcaaaatc caaaggttca aaggtcaaaa ggcagccctt cgtccttcga ccctttgctc
     1501 ttcactgatc actgttcact gattactgat gactgaatac cgacactacc cgatcgcctc
     1561 ctcccctcgt tctttctcat aaaccgcccg tctgtcgggc aggtattcgg gtacgggcat
     1621 gtcccaccag ccggtcggcg tgacaccgcg cgcgacctcg cgatcgacca tgacttcgat
     1681 cagcgccggg cccttcgtcg ccagcgcacg cttgaccgcc ggctgcactt cgccgggcgc
     1741 gtggatgcgc tccgagtaca ggccgaaagc gcgcgccgcc tcgcacaagt tgggcgtgta
     1801 caggccgccg tccttcttct gaaaatcgac gaccgtcgtc cgcccgaagt tgtgcatctg
     1861 cccgcccttg atcgagatga agccgctgtt gttcagcacg acgaacagca caggcaggtc
     1921 aaccatcgcg gcgacggcca tttcctgcat cgtctgcatg aagtcgccgt cgccggcgca
     1981 gccgaccacc tgccggccgg ggtgcgccaa ctgcgcgccg atcgccgccg gcaccgtgaa
     2041 gcccatcgtg ctgaagccgc cgctggtgat gtgcgtgcgc ggctcgtaca ccgggaaggc
     2101 ctgccggacg acgccctgca cgtggcccgc gccggaggtg acgatcgcat cgcggtcgag
     2161 cgcggcgcgc agttccttca tcacccgcgc ctgcgtcacc ggcgtcgcgt cggaatcgcg
     2221 attcgcgccc aggacgcgat cccagtcggc tttcttcgcc tgaatctcgg cgaagtaact
     2281 gctcgacgag tagtctttgg ccttgcccac ctgccgggcg gcgtcgagca gatcggccag
     2341 cccggctttc gcatcggcca ccagcccgat ctcggccggg tagttcttgc cgatctcgcc
     2401 cgggtcgatg tcgaggtgaa tcaatttcgt cggcgggatg gagtacgtca cgccctgccg
     2461 gaacgacgac gacgtccaat cgacgaagcg gcagccggcg gcgagcagaa cgtcggccgt
     2521 cgaggcgagc tggttgccgc agtggctggc ctgcgtgccg acgccccagg cgttcagggg
     2581 gtggtcttcg ggaatcgcgc ccttgccctg ccatgtcgtc acgattggcg cgccgaggta
     2641 ttcggccagc gctacgagtt ccttggccgc attagacaga atgacgccgc cgccggcgac
     2701 gatcaccggg cgccgtgcgc cgtagagcag acgcgcagcg cgctgcgtga gatcggcgtc
     2761 agggcgcggc cgggcggcgg gcacgcgccg gtcgaaaccg ccgggttgat catcgggcgg
     2821 gaggtccacg tcggcggctt ccgcttgcac gtccatcggc aggtcgaggt gcaccgggcc
     2881 gggccggccg ctcagcatgg cgttgaatgc gcgatgcatg atgtgcggca gcccctgcgc
     2941 cgagacggcg aggtattgtc gcttgctcac cggctcgaag atgcgcaggt tgttcgacgc
     3001 ctgcgcccgg tcaagttcct gcatcacggc gtggccgcgc atgtacgtgt gcgtcgagcc
     3061 ggtcatcaat agcacggcgc tcgagtcgac gaaggcagtt gccatgccga tgacggtgtt
     3121 ggtcgagccg gggccgatcg aggtgaacgc ggcgagcggg cggcccgtcg cgcggaagta
     3181 gccgtcggcg acgtgcaccg ccgactgctc gtgcatcacc atcagagtcg tgagttggtc
     3241 gcgcacgtcg aggaaggcgt cggtcaggca ccagcagccg tggccgggaa tgccgagcgc
     3301 gtacggcacg ccggccttca caagcgagtg agcgataatc tgcgcgccgg tgagttgcat
     3361 atcacctcct tgctcgcttc cgctcccaga ccgccggatt gatcacgcga agcggagcgg
     3421 gggcgggggc ggagatggag agaagttaaa tccactcctc cgccgtcgtc ttcagcacga
     3481 tcggcctgag aaactcgacg ctcttgacga tgccctcgcg cgaccccatc agccggtctt
     3541 cgtgctcgat gctcaacacg ccgtcgtagc ccatcaggcg cagcgtgctg acgaagtcgc
     3601 gccaccaggc cgcgccgtgc ccgtaaccga cggtgcgaaa tgcccacgac cgctcgccta
     3661 ccatcgacgt ggggcgcgtg tccagcccgc cgttgagcgc catctcctgc ggatcgattc
     3721 gcgtgtcttt ggcatggacg tggaacacgc cgttgcgccc cagcgcgcgc acgacgacga
     3781 gcgggtccat gccctgccag aataaatggc tcggatcgag gttcgcgccg atgcctgggc
     3841 cggcgacgtc gcgcagccgc agcagcgagc gcgtattgta caccgactgg ccgggatgca
     3901 tctcgatcgc gattttcacg ccgcgatcgg cggcgaatcg gcccgccttt ttccagaacg
     3961 gctcgaccac ttcggcccat tgctgctcgc ccaactcggc gaactcgcgc tgccaggtgc
     4021 acgtcaccca gttcggatac tccccgccgg tcggatcgcc ggggcagccg ctcatcgtga
     4081 caatggtgtc cagcccaagc cggttggccg cttccaccgt cttgaagaac gtgtcctgag
     4141 atttcttccc gcgctccggg tgaggatcga gcaggttgcc attgcagttg agcgcgctga
     4201 gcgtcagccc gcggctctca atcgcgtcct tgaaccttgc gcgcgcgtcc gcgtcgttca
     4261 gcagccgatc gacgtcgcag tgcggcgcgt tcgagaaatt tccggtgccg atctcgaccg
     4321 cttcgatccc ctgcccggcc acccaatcca gcgcttcggc gaacgacaga tcttgcagaa
     4381 cgtcggtgaa cagtccgatt ttcaaaatgg cctccgcggg tgagggccgg catggggatg
     4441 ccggccctac ggggttttca cg
//
LOCUS       DBNHXB010000214        18072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2062405, whole genome shotgun sequence.
ACCESSION   DBNHXB010000214 DBNHXB010000000
VERSION     DBNHXB010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 18072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18072
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2062405"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="AC162G_08825"
     CDS             complement(<1..524)
                     /locus_tag="AC162G_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR5075957.1"
                     /translation="MLKLFRKGTDETPRPPRLWLDLGDLGDLVNPAGPHEQWQDHGLG
                     LLRTILHQNGIMTDLASTRACTSWNEVRGKLKGYEMLIMNVRSYTFPAAYQAAKLFKE
                     VNPNGLVLTGGMHATVALDEMTAVAEFDKICQGPGENVIVDLVSHPEKHPRVVLGVGA
                     KSMEEWPMIDRRLWP"
     gene            complement(545..928)
                     /locus_tag="AC162G_08830"
     CDS             complement(545..928)
                     /locus_tag="AC162G_08830"
                     /inference="COORDINATES: protein motif:HMM:NF021486.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 family protein"
                     /protein_id="HIR5075958.1"
                     /translation="MSRHFSVEEANALLPRIRSIVESVLAARQRILDAQPEVWPALEK
                     AIGNGGSKRASALIEDFKKVENGVKTIQKLGAVVKDVNTGLIDFPSLRGEREVFLCWR
                     YDEPAVAHWHELHTGFAGRQAIDWR"
     gene            complement(1058..1489)
                     /gene="erpA"
                     /locus_tag="AC162G_08835"
     CDS             complement(1058..1489)
                     /gene="erpA"
                     /locus_tag="AC162G_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HIR5075959.1"
                     /translation="MTTAPILMHDAEQTLTETPLLTITPAAAQKLVKLLAEKNIPDYG
                     LRVFVAGGGCSGMQYGMAFEQTAREFDAVVELHGVRLFVDPTSAQYLGDATIDYVDNL
                     MGGGFRIDNPNAVATCGCGHSFRTQDSGNSAYGAGGGSGCS"
     gene            complement(1735..2358)
                     /locus_tag="AC162G_08840"
     CDS             complement(1735..2358)
                     /locus_tag="AC162G_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024804.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5075960.1"
                     /translation="MQATRQAILKILKERGQATVEQMSDELNLTPVTIRHHLDILRSE
                     GLVQAPKVQRRQSPGRPQHVYGLTEQATNYFPKNYVAFADLTLQELRERVSTDEMDTI
                     VRGVARRMAANAPEPLPGEPMPERLTRIVDFLNRQGYVARWEPNDQGFLLHMANCPYQ
                     GLTDHHAAPCIMDSALLTDLLGSQPLRISWMSAGDDTCTYLVKNSVH"
     gene            2429..2821
                     /gene="iscX"
                     /locus_tag="AC162G_08845"
     CDS             2429..2821
                     /gene="iscX"
                     /locus_tag="AC162G_08845"
                     /inference="COORDINATES: protein motif:HMM:NF016284.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HIR5075961.1"
                     /translation="MSIGKMRVIKKKVRCRFLRNLVQLAQTRPEVQLRARHECGFAAR
                     AGNSIGDVKLQRDQAAQVDEFYWDDPYPIALELFRRHPGVDPLSVSWETLHRWVVELP
                     QFADDASVTSLNLLEDIQKEWYEEVTSR"
     gene            2818..3462
                     /locus_tag="AC162G_08850"
     CDS             2818..3462
                     /locus_tag="AC162G_08850"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR5075962.1"
                     /translation="MTDANEQRTPVNDPMVPPELQNNVFITTLDKLYNWGRKRSVWPL
                     MFGLACCAIEMISTAAGRFDLDRFGMGLMRASPRQADLMIVSGTVTKKMVPQIVRLYN
                     QMAEPKYVLSMGACATGGGPFKEGYNVVSGIDKFLPVDVYVPGCPPTPQALLNGMLKL
                     HEKMEKQSIRTVSWYKKDVGEAIPVPVLGPDLIDVRRVPEIRSSLAKVSEPSQG"
     gene            3474..3869
                     /locus_tag="AC162G_08855"
                     /pseudo
     CDS             3474..3869
                     /locus_tag="AC162G_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012549.8"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
     gene            4125..5273
                     /locus_tag="AC162G_08860"
     CDS             4125..5273
                     /locus_tag="AC162G_08860"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HIR5075963.1"
                     /translation="MTVPTAGGNGDSAAGLRTDQLVVNLGPQHPSTHGVFRMAVRLDG
                     ETIRELEPVVGYLHRNHEQIGERNTYIQNMPYTDRLDYICSMSNNVGYAITVEKLMGL
                     KPTERAEYLRVIMAELTRIVNHFWAIGFLLNDLGAFFTPALYAIEERELILDLFEAAS
                     GSRMMCNYMRFGGVVRDVPPGWLEKCREIVFERLPRKIDHLDTFLSGNEIIRARCMGV
                     GYLSPQDAIAFSTAGPVLRASGVPYDVRRAESYSIYDRFDFDVAVRYNGDVYDRYVIR
                     IDEMRQSVRILQQALEQIPEGEIQAGKKQYSQRVPAGQAYGRVENPKGELGFYVVSDG
                     SANPYRYHIRAPSFINLTALERMCVGYKVADTVIILGSIDIVLGETDR"
     gene            5303..5983
                     /locus_tag="AC162G_08865"
     CDS             5303..5983
                     /locus_tag="AC162G_08865"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR5075964.1"
                     /translation="MFGTGVLRGLGVTFKHFVASYVDDIAYLFRGGRYYNEKALRQRQ
                     GPKARGVFTVQYPEEKLPVPENFRYLPFLIYDTTANDDKCTSCGICAKVCPPQCIWIV
                     RTNDPVTKRPVPQPAEFYIDTTICMQCGFCAEFCPFDAIKMDHDYEIATFERFQTLIF
                     DKQKLTKPDTYHAAIHPTDWAREQVELAEKAAKEAEKKRQAEEKAKAAAAAKAAAVAQ
                     PPPAHGAS"
     gene            6115..7161
                     /locus_tag="AC162G_08870"
     CDS             6115..7161
                     /locus_tag="AC162G_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006278553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HIR5075965.1"
                     /translation="MANVTETQVIEALSRVKEPELGRNIVSLGMIQDLKIDGGHVGFT
                     IVLTTPACPLTDVLDRDARAELGKLPGVKEIDIKWDSKVPEDQRIAGQLGGQFKNTIA
                     VSSGKGGVGKTTVAVNLAVALAQAGATVGLLDADIYGPNVPLMLGIDSMPKPRDGRLI
                     PAYAHGLRVMSMGFLVPPDQPVIWRGPMIHSAIRQFLGDVDWGNPDYLIIDLPPGTGD
                     AQLTLAQSVPLTGAIIVTTPQEVALADAVKGFATFEKLEVDVLGIVENMGPYTDPKTG
                     QTISFFGEGGGQRMAERLGVPFLGTVPLDPNVRIGGDDGTPVVVQHPDSPAAQMFMKI
                     AKDVAARISVLNLS"
     gene            complement(7254..7379)
                     /locus_tag="AC162G_08875"
     CDS             complement(7254..7379)
                     /locus_tag="AC162G_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075966.1"
                     /translation="MIDKTNFQAIEERFEQLAAAIVVTLLATSILGFVLLLIDLP"
     gene            complement(7801..8553)
                     /locus_tag="AC162G_08880"
     CDS             complement(7801..8553)
                     /locus_tag="AC162G_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HIR5075967.1"
                     /translation="MPHAHFRFYEELNDFLPPGRRKVTFVHEWKGIASVKDIVEALGV
                     PHTEVDLILANGESVDFAYRPKDGDRISVYPMFESIDISPVQRLRPRPLREPRFVLDG
                     HLGRLARHLRMLGFDTLWSNDADDERLAHISQQQTRTLLTRDQGLLKRRDVTRGYWVR
                     STAPREQLREVLERFDLYRAATPFTRCMACNGLLRSVTLEEVKERLPSRTAQHYREFR
                     QCEQCAKVYWEGPHVRRMEKLLQEVLGSSAVE"
     gene            8649..10085
                     /locus_tag="AC162G_08885"
     CDS             8649..10085
                     /locus_tag="AC162G_08885"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR5075968.1"
                     /translation="MTAHFSSQVLFEPISGQKTFGPDLPDYDALTDCIRCGRCLPVCP
                     TYQETALETQSPRGRLNLLRAVEDGKIALDHARVEEHLYHCLDCRACNMVCPAGIPIG
                     ELIVQGRAAVEHAHRRNWLIRFALNVVLTSPRRAEVLTAPLRLIQKLKLDQLGVVLFG
                     WLPKIGPALRDLVHMAPAISRPIRPELARRPSSKGPDHPTTGLLDYSTTRPPDYSTTR
                     PPGHSANRRVGFFLGCVMNVAFADASRASVALMQRFGCEVVTPADQACCGAPHDDQGM
                     KNRARAFTRRNIEAFERLGDLAAIVADCAACSGFLKEYAHALGDDPEWAERAERFSSK
                     VRDITEWLDEIIPDDRSAGFSLPVGTPHRVVTYHEPCHLAHMQGVRRQPRALLARAEN
                     LDVRELPDATRCCGSAGIYNLTHPAMSKALLGRKMADVASTGAGVVVSANPGCLIQLE
                     WGRRWHKLPVAVKHITEVILESLVTREA"
     gene            10190..11161
                     /locus_tag="AC162G_08890"
     CDS             10190..11161
                     /locus_tag="AC162G_08890"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HIR5075969.1"
                     /translation="MDVSVTQTLHREQTLVGVRFMPVGKIYHFDATRFPDLREGDWVI
                     VTTSRGRQMGEVATFDLPKHGDADGPFKAIDRIATNRDLAVRHYWAQKETEAMVIGRE
                     KVKELGLPVKIVKAEYAFDGSRLAFLYSAEEEKPELGTLQQDLQRSFKAKVELRLVGP
                     RDTAKIIGGSGACGLEVRCCSAFLTEFSPISIKMAKEQGISLNPQEITGMCGRLRCCL
                     IYEYEQYVDARRTLPRRGKEVGTPHGTGKVVDLLPLKESALVQIGEATFVVHREDIQP
                     LAELEAFQKKAASPCGEGTGCTCGHAKKKKRKSQSSGSGDGGDGGAE"
     gene            11136..12545
                     /locus_tag="AC162G_08895"
     CDS             11136..12545
                     /locus_tag="AC162G_08895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5075970.1"
                     /translation="MAATAALNELRAAIGAERVSTDPAELIVYAYDGAWVEGRPAVVL
                     HPETTRHVAESLRIADRERIPVVPRGAATGLAGGAVAVEGGWCLNLARMNKILDISAA
                     DSVAVVQPGVVTAALQKEVEKRGLFYPPDPASLHMCTIGGNVSTNAGGPRCLKYGVTA
                     DYVLALEVVLPGGRVMRTGRRTIKDVAGYNLTQLFVGSEGTLGAITEITLQLIPKPAA
                     EATALAAFPRIADACQAVGRILASGIVPLVTELMDRIVVRAVDAYYRSRSASAGLPLD
                     DDAVLLVAVDGDGDQVERDIDTIAGLLRGSGASEVRRARDAVEAEALWEARRAINPAL
                     IRLAHDRLGEDIVVPRGHIPRMVERLRRIAAEHDLTVALFGHIGDGNLHPTILCDRRD
                     GEMMKRVQSASGAIFGAALDLGGSITGEHGIGIVKLDYLQRAVDPIALEAMQAIKRIF
                     DPNGIMNPGKKLPSGVGEW"
     gene            12607..13629
                     /locus_tag="AC162G_08900"
     CDS             12607..13629
                     /locus_tag="AC162G_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010474105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-methyltransferase"
                     /protein_id="HIR5075971.1"
                     /translation="MRELTEKARAPRNRTITLSTDEATFYQKRLLKLSRSASAVDLEN
                     KTINQDLYEVLDWLPASFVDLLFIDPPYNLTKSFNTTAFNERSTVEYRDWFESWFPKL
                     LKTLKPSASVYICGDWRSSTAIHLVAEKYLIVRNRITWEREKGRGARANWKNCSEDIW
                     FCTVSETYTYNLDAVKLKRRVMAPYTDSEGNPKDWDKTESGNYRLTHPSNVWTDLTVP
                     FWSMPENTDHPTQKPEKLVAKIILSSSKPGDLVFDPFLGSGTTSVVAKKLSRRYVGIE
                     IDPAYCCLAEKRLAMAEYEKSIQGYAEGVFWERNSLQDRRWSLRKRGGQSVLPLFAQG
                     LDKEHE"
     gene            13626..14180
                     /locus_tag="AC162G_08905"
     CDS             13626..14180
                     /locus_tag="AC162G_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075972.1"
                     /translation="MSKSRIFHTGRYKAIQKAIVKLLNSQPDFVSTRTAASTRAVGDA
                     IQTILSERFQSLLGELCAEYSADFARRAMADLAFTDRDGLYHVVDVKTHRLGTQFNMP
                     NLTSVERLARFYEGDENYFVCVVREIRYRRDEGQDRTGAFCSDRVSLLGLPDDWSAGM
                     GTDTDSQRECYQYQRRLFSQEMDD"
     gene            14323..15024
                     /locus_tag="AC162G_08910"
     CDS             14323..15024
                     /locus_tag="AC162G_08910"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR5075973.1"
                     /translation="MADEGLRVLNRVMVILAHPDDPEFFCGATIAKWAKEGRHIRYCM
                     LTCGDKGSDDPDMAHDRLCAIRHEEQTNAARVLGVTDLVWLNYPDGELVPTLETRLRV
                     AREIRKFKPDVVVTCDPTRVITAFGTVNHPDHRAAGQIALDAIMPGAGNRMYFTELLS
                     EGLEPHQVKEVYIAGALEPDTAIDVTDTFDVKLRALREHKSQIKDPDGLEQRMRARFS
                     RDGIFVEMFKRLVLA"
     gene            15111..15866
                     /locus_tag="AC162G_08915"
     CDS             15111..15866
                     /locus_tag="AC162G_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5075974.1"
                     /translation="MADLLLLGTGAALNDGSREPTMLALRGASSTVLIDCGSNPVRQL
                     QRLGVPLASIERLILTHSHPDHTSGFALLVEMLWLAGRREPLPVHGPAEAIDVVRRVF
                     AQWDTSAWNGLPDLQWHEARLEIGAPIAAGADFELTAAPGKHSVPVIGVRARDMHGGG
                     ALAYGADGEPSPGIRALAQGADLLVHEATGAYPTHSTAEGAAGQASAAGVKRLVLVHL
                     SPAVNDLETQRLAANKIFGGEVFVGRDLDRYAF"
     gene            16012..17223
                     /locus_tag="AC162G_08920"
     CDS             16012..17223
                     /locus_tag="AC162G_08920"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR5075975.1"
                     /translation="MSTIDFKPEALAMKEQLVAWRRDFHRHPELAYQEVRTSGIVAKH
                     LGDLGLEVQTGVGKTGVIGVLDGAQPGPVVMLRFDMDALPIHEANRTDYVSQNPGVMH
                     ACGHDAHTAIGMGIAQLLTKHRDRLCGAVKFVFQPAEEGGNGALAMINDGALEGPRAA
                     VSMGLHVWNEMPVGQAGVGAGPIMAAAGILKIKVQGKGGHGAQPDKAIDAILAGSAIV
                     NALQTIVARNVEPRQTAVVSVCSFHAGDAFNVIADSAELIGTYRSFDDGVQELIMCRI
                     REVAEDTARAMGAMVTVELRPITSTTVNDARVAEFVRADASDVLGADRVVPEQFTMGA
                     EDMSEFLKRVPGCFFFLGSRNDERGFNAPHHNPRFDIDENVMPLGVAILAQSATRYLS
                     GQGKVGTPSVP"
     gene            17277..17960
                     /locus_tag="AC162G_08925"
     CDS             17277..17960
                     /locus_tag="AC162G_08925"
                     /inference="COORDINATES: protein motif:HMM:NF015534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HIR5075976.1"
                     /translation="MTQAGPLRWRTGNGWLVLVGGGRWNTTEAIDRRAIDAMTDEAPI
                     AFVPAAGCPPDYGESFLAHYARLGAPAGYVVPIHDQASANDPANVRRLTHAGLVYFGG
                     GDMRQLLDAMTGAQALDAVAAAYAAGAVIVGMSAGAAALAAWGLSSDPGVGLLRGWGW
                     LPNAIVAPHYAPAQADALHAGLREHPQLLGLGIPEDVALALGPDAEVFTWGRGQITVT
                     LGSKFDAEG"
BASE COUNT         3489 a         5500 c         5753 g         3330 t
ORIGIN      
        1 ggccagagcc tccggtcgat catcggccac tcttccatcg acttcgcgcc gacgccgagg
       61 acgacgcgcg ggtgcttctc cggatggctc accagatcga cgatgacgtt ttcgccggga
      121 ccctggcata tcttgtcgaa ctccgccacc gccgtcattt cgtcgagcgc gaccgtggcg
      181 tgcatcccgc cggtgagcac cagtccgttc ggattcacct ccttgaacag cttcgccgcc
      241 tggtaggctg cgggaaacgt gtagctcctc acattcatga tgagcatctc gtatcccttg
      301 agcttgccgc gcacttcgtt ccatgacgtg caggcgcgcg tcgatgccag atcggtcata
      361 atcccgttct ggtgcaagat cgtcctgagg agtccaagtc cgtgatcctg ccattgctcg
      421 tgcggtccgg ccgggttgac caggtcgccc agatcgccca ggtccagcca caatctcggc
      481 ggcctgggcg tctcgtctgt tccctttcgg aagagtttga gcacggtccg attctcctcg
      541 acgatcaccg ccagtcgatg gcctggcgcc cggcaaagcc ggtgtgcagc tcgtgccagt
      601 gggcgactgc cggctcgtcg tagcgccagc atagaaagac ttcccgttct cctcgcagag
      661 acgggaagtc tatcaggccg gtgttgacat cctttacgac ggcgcccagc ttctgaatgg
      721 tcttgacgcc gttttcgacc ttcttgaaat cctcgatcag cgcgctcgct ctcttgctgc
      781 cgccgttgcc gatcgccttc tcgagcgccg gccagacttc gggctgcgcg tcgaggatac
      841 gctgccgggc ggcgagcacc gattcgacga tagatcggat gcggggcagc agcgcgtttg
      901 cctcttcgac gctgaaatgg cgggacatgc cagcttaact gcctatccga aaactagcgg
      961 gctaggctcc cacgcccagc catccggcgg catgcccact ccgctgcgtt acggggtgct
     1021 tcgcaagtag ccgcctccgc tgggtctcgg acaagcacta actgcagccg gacccaccgc
     1081 ccgcgccgta cgccgaattg ccgctgtcct gtgtacggaa ggagtgccca cagccgcacg
     1141 tcgcaaccgc gttcgggttg tcaatgcgaa aaccgccgcc catcaggttg tcgacgtagt
     1201 cgatcgtggc atcccccagg tattgcgcgc tggtcggatc gacgaacaac ctcacgccgt
     1261 gcagttcgac gacggcgtcg aactcacgcg ccgtttgctc gaaggccatt ccatattgca
     1321 tgcccgagca tccgccgccg gctacgaaca cacgaagccc gtagtccggg atattctttt
     1381 cggccagcag cttgaccagc ttctgcgctg ccgccggcgt gatagtcagg agcggcgtct
     1441 cggtcagtgt ctgctcggcg tcgtgcataa gtattggggc ggttgtcatg gtcatctcca
     1501 caatatggaa cgaatcacct gctaaggcca ggggacttct aacatcccca ggcgcatttg
     1561 tcaataaagc aggtgtccat ttgcttaatt cggtcctcaa gtctaacttc aggtgctcgt
     1621 caggggttta tcccacaggg aggcttcgca ctgaccgccg accccgatcg gtcgggtcac
     1681 ggacccgacc ggagcaacaa ggtgaacgcc gttcacgacg acgactgggt ccggctagtg
     1741 aacggaattc ttcaccaggt acgtgcaggt gtcgtcgcca gcgctcatcc aagaaatgcg
     1801 aagcggctgc gatccgagca gatccgtcag cagagccgaa tccatgatac aaggcgcggc
     1861 gtggtgatcc gtcagtccct ggtatgggca gtttgccata tgcagcagga agccctgatc
     1921 gttgggttcc cagcgcgcga cgtaaccctg ccgattgaga aagtccacga tgcgtgttag
     1981 ccgctctggc ataggctcgc ccggcagagg ctcgggcgcg ttggccgcca tgcgccgcgc
     2041 caccccgcgc acgatcgtgt ccatttcgtc ggtgctgacg cgctcgcgca gctcctggag
     2101 cgtgaggtct gcaaacgcga cgtagttctt gggaaagtag ttggtcgcct gttcggtgag
     2161 gccgtagacg tgctgcgggc ggcccggcga ctgccgccgc tgcaccttgg gtgcctgcac
     2221 gaggccctcg ctgcgcagaa tgtcaagatg gtggcggatc gtgaccggcg tcaggttaag
     2281 ctcgtcgctc atctgctcga cggtcgcctg accgcgctcc ttcaggatct tgagtattgc
     2341 ctgtcgtgtg gcctgcatgg ctgatcctac ctttcatgtg cgccaccccc caaacggctg
     2401 caacctaatt ctagcacgtt gaccccgaat gtcaattggc aaaatgcgcg taattaaaaa
     2461 gaaagtgcgt tgccgttttt tgagaaacct agtacaattg gcccaaaccc gccccgaagt
     2521 ccagctcagg gcacgccatg agtgtggctt cgctgcgcgg gctggaaatt ccatcggcga
     2581 tgtcaaattg cagcgcgatc aggcggcgca ggtggacgag ttctactggg acgatcccta
     2641 tccgattgcg ctcgaactgt ttcgccggca tcctggcgtc gatccgctat cggtgtcctg
     2701 ggagacgctt catcgctggg tggtagaact gccgcagttt gcagacgacg caagcgtgac
     2761 cagtctgaac ttgttggaag acatccaaaa ggaatggtat gaggaggtca cttcacgatg
     2821 actgacgcga atgaacagcg gacgcccgtc aacgatccga tggtgccccc cgagctgcag
     2881 aacaacgtct tcatcacgac attggataag ctgtacaact ggggacggaa gcgcagcgtc
     2941 tggccgctga tgttcggcct ggcgtgctgc gccatcgaga tgatttccac ggcggccggc
     3001 cgcttcgacc tagaccggtt cggcatgggc ctcatgcgcg ccagcccgcg ccaggctgac
     3061 ctgatgatcg tctccggcac ggtgacgaag aagatggtgc cgcagatcgt gcgcctgtac
     3121 aaccagatgg ccgagccgaa gtacgtgcta tcgatgggcg cgtgcgccac gggcggcggc
     3181 ccattcaaag agggctacaa cgtcgtgtcc ggcatcgaca agttcctgcc cgtcgacgtg
     3241 tatgtgccgg gctgcccgcc gacgccgcag gccctgctca acggcatgct caagctgcac
     3301 gagaaaatgg aaaagcagag cattcgcacc gtgtcgtggt acaagaaaga tgtcggcgag
     3361 gcgattccag ttcccgtcct gggacctgac ctcatcgacg tgcggcgcgt tcctgaaatc
     3421 cgttccagcc ttgcgaaggt ttcagaacct tcgcaaggtt agccgaggtc ctcatggcaa
     3481 acccaaccac cgctcctgcc gccgaattga ccggcccact cgccgcgttg aaagagaagt
     3541 tcggcgacgc cattcagcct gccgacttcc aaggcgtcac gatcgacccg gcgcatctcg
     3601 tcgaagtggc gaaaaccatt cgcgacgatc tgggctacaa ctacctgtcc agcgtcacgg
     3661 cggtcgacta catcaaggac ggccagatgg aagtggtgta ccacgccttc tcgatcgaca
     3721 aaggcggcgg cccgctggtc ttcaaggcca gggcgccgcg cgacaacgcc gtgctgcctt
     3781 cgctgacctc ggtatggccg ggcgccgatt ttcaggagcg cgaagcctgg gacttgtttg
     3841 gcgtcaagtt cgagggccat ccgaacctca agcgcatcct gatgtgggaa ggcttcgacg
     3901 gccacccgat gcgcaaagac tggcatgaag cgtattacga ggaggatagc aagcctttcg
     3961 acagccgctg gccgggcggg cgccacgtgc gcgccgaaga ccgcgtgccg tgggccgata
     4021 acgtcagcta tccgccaggg ttcgatccga gcgcctttgt cgaggaagcc aacgcggccg
     4081 tgtacgagcg cctgcgccag gtcgaggtcg tccagggcca gccgctgacg gttccgaccg
     4141 ccggcggcaa cggggatagc gcggccggtc tcagaaccga ccaactcgtc gtcaacctgg
     4201 gcccacagca tccttcgacg cacggcgtct ttcgcatggc cgtccggctg gacggcgaga
     4261 ccatccgcga gctggagccg gttgtcggct acctgcaccg caaccacgag cagatcggcg
     4321 agcgcaatac ttacattcag aacatgccgt acaccgatcg gctcgactac atctgctcga
     4381 tgagcaacaa cgtgggttac gccatcaccg tcgagaagct gatgggcctc aagccgactg
     4441 agcgcgccga gtacctgcgc gtgatcatgg ccgagctgac gcgcatcgtc aatcactttt
     4501 gggcgatcgg cttcctgctg aacgacctgg gcgcgttctt cacgccggcg ctgtacgcca
     4561 tcgaggagcg tgagctgatc ctcgacctgt tcgaggcggc gtcgggcagc cgcatgatgt
     4621 gcaactacat gcgcttcggc ggcgtggtac gcgacgtgcc gcccggctgg cttgagaagt
     4681 gtcgcgagat cgttttcgag cggctgccga ggaagatcga tcacctcgat accttcctat
     4741 ccggcaacga gatcatccgc gcgcgctgta tgggcgtcgg atatttatct ccccaggacg
     4801 cgatcgcgtt cagcacggcc ggcccggtgc tgcgcgcctc cggggtgccg tacgacgtgc
     4861 gccgcgcgga gtcgtattcg atctacgatc gcttcgactt cgacgtggcc gtgcgctaca
     4921 acggcgacgt gtacgatcgt tacgtcatcc gtatcgacga gatgcggcag tcggtgcgta
     4981 tcctccagca ggcgctcgag cagattcccg agggtgagat ccaggctggc aagaagcagt
     5041 attcgcagcg cgtgcccgcc ggtcaggcct acgggcgcgt cgagaacccc aaaggcgagc
     5101 tgggcttcta cgtcgtgtcg gacgggtcgg cgaacccgta tcgctatcac atccgggcgc
     5161 cttcgtttat caacctgacg gcgctggagc ggatgtgtgt cggatacaag gtcgccgaca
     5221 cggtcatcat tttgggcagc atcgatattg tgctgggcga aaccgatcgt tagcgcccgg
     5281 tcgttcagtg gaggaaacca cgatgttcgg aaccggtgtg ctcaggggtc tgggtgtgac
     5341 gttcaagcat ttcgtcgcgt catacgtgga cgacatcgcc tatctgtttc gcggcgggcg
     5401 ctactacaac gaaaaggcgc tgcgtcagcg gcaggggccg aaggcgaggg gcgtgttcac
     5461 cgtgcagtat cccgaagaga agctgcctgt gccggagaac ttccgctacc tgccgttcct
     5521 gatctacgac acaacggcga acgacgacaa gtgcacgtcg tgcggcatct gcgccaaggt
     5581 ttgcccgccg cagtgcatct ggatcgtgcg caccaacgac ccggtcacca agcggcccgt
     5641 cccgcagccc gccgagttct acatcgacac gacaatctgc atgcagtgcg ggttctgcgc
     5701 cgagttctgc ccgttcgacg cgatcaagat ggaccacgac tatgagatcg cgacattcga
     5761 gcgcttccag acgctcatct tcgacaagca gaagctgacc aagcccgata cgtatcacgc
     5821 ggcgatccac ccgaccgact gggcgcgcga gcaggtggag ctggccgaga aggcggcgaa
     5881 ggaggccgag aagaagaggc aggccgagga gaaggccaag gcggccgccg cggcgaaagc
     5941 ggccgcggtt gcgcagccgc cgccggctca tggtgcgtcg tgatgagtgc gatgtgaggc
     6001 atgatgggcc gactctatcc cctggtccgc cctcgccggg tgcgattgcg ggttccggtc
     6061 gagggcggta ttgtcgcgaa gccgggcgaa gcgatggcgc atcgggagta aagcatggcg
     6121 aacgtgactg aaacacaagt gatcgaggcg ctgagccggg tgaaagaacc cgagctagga
     6181 cgaaacatcg tgtcgctggg catgattcag gatctcaaga tcgacggcgg ccatgtcggc
     6241 ttcacgatcg tcctgacgac gccggcctgc ccgctgaccg acgtattgga cagggacgct
     6301 cgtgcggagc tgggcaagct gccgggcgtg aaagagatcg acatcaaatg ggactcgaaa
     6361 gtgcccgaag accagcgcat cgccggccag ctcggcggac agtttaagaa cacgatcgcc
     6421 gtatcgagcg gcaagggcgg cgtcggcaag acgacggttg cggtcaatct cgccgtcgcg
     6481 ctggcgcagg ctggcgcgac cgtgggcctg ctcgacgccg acatctacgg gccaaacgtc
     6541 ccgctcatgc tcggcatcga cagcatgccc aagccgcgcg acggccggct cataccggct
     6601 tacgcgcacg ggctgcgagt catgagcatg ggcttcctcg tcccgcccga ccagccggtg
     6661 atctggcgcg ggccgatgat tcactcggcg atccgccagt tcctcggcga cgtcgactgg
     6721 ggcaaccccg actacctgat catcgatctt ccaccgggca ccggcgacgc acagcttact
     6781 ctcgcgcagt cggtcccgct caccggcgcg atcatcgtca ccacgccaca ggaagtcgcg
     6841 ctcgccgacg cggtgaaggg ctttgccacg ttcgagaagc tggaggtcga cgttctcggc
     6901 attgtcgaga acatggggcc gtataccgat ccgaagaccg ggcagacaat ctccttcttc
     6961 ggcgaaggcg gcggccagcg catggccgag cggctgggcg tgcctttcct ggggaccgtc
     7021 ccgctcgatc cgaacgtgcg catcggcggc gacgacggca cgccggtcgt cgtgcagcac
     7081 cccgattcgc cggcggcgca gatgttcatg aagatcgcga aggatgtcgc cgcgagaatc
     7141 agcgtgctga atctatcgtg atgccaagcg gggatcccta tgcccgctgt tgaggcatag
     7201 ggatgcgtca accacggatt tttggacagt cgctggaatt cgagaaccgt ggcctatggc
     7261 aggtcgatga ggaggagcac gaaaccgagg atggatgttg cgaggagagt tacgacaatg
     7321 gctgccgcca gttgctcgaa acgttcctcg atggcttgga agttcgtttt atcgatcatc
     7381 gtgacagtct ccttagacta taaacctcaa ccatcttgat ccgttgaagg catttgtcca
     7441 actgcggagc atcttcgatg ttggacacgc ccctctatgg tttcagagta tcacgcttat
     7501 ctggtgttgg aaatgagaaa gaagtttatc ccgatggact ttgcgatacg gatgacaaaa
     7561 atgcatctag gctcatattg gaaaggtcgc tctatttgta gctctatttt ctctgggcct
     7621 gcttccatca cggggatcaa gaactacaaa gtctggtaca aaaagcccat ctggtgatct
     7681 gattgttgtg tcccggtacc tttggatgat tccacacgta tcataccaga tgaacatgaa
     7741 ggcgccatga agcgaagatg aagttttccg agtttgaagc agaccttttt gaggttgtgt
     7801 ctattcgacg gcggaactcc ccaggacctc ctgcagcagc ttctccatgc gccgcacgtg
     7861 cggtccctcc caatacactt tcgcacattg ctcgcactgc cggaactcac ggtaatgctg
     7921 cgccgtgcga gacggtagac gctccttgac ctcttcgagc gtgacggatc gcagcagccc
     7981 attgcaagcc atacagcggg tgaagggcgt tgcggcgcga tacaaatcga agcgctccag
     8041 cacctccctc aattgctcac gcggcgcggt cgatcgcacc cagtagccgc gcgtcacgtc
     8101 cctccgcttg agaagcccct ggtcgcgcgt gagcaaggtg cgcgtttgct gctgcgagat
     8161 atgcgccagc ctctcgtcgt cggcgtcgtt gctccacagt gtatcgaagc ccagcatgcg
     8221 cagatgccgg gcgagacggc ccaggtggcc atcgagcacg aagcgcggct cgcgcagcgg
     8281 gcgcgggcgc agccgctgca ccggcgagat gtcgattgac tcgaacattg ggtagacgct
     8341 gatccggtcg ccgtctttcg gtcgataggc aaagtcaacc gactcgccgt tggcgaggat
     8401 gagatccacc tcggtgtgag gcacgcccag cgcctcgacg atgtctttca ccgaggcgat
     8461 gcccttccac tcgtgcacga aggtcacctt gcgcctgccc ggcgggagga agtcgttcag
     8521 ctcttcgtag aaacggaagt gggcgtgagg catgggcagt tgtgactttc gaggatgttt
     8581 tcgattggtg tgtcggcgtc gggcttgcgt ttaggcatgg atggaataca ctatgctagt
     8641 aatagtctat gacagctcat ttttcgagtc aagtactgtt cgagccgata tcgggtcaga
     8701 aaaccttcgg cccagacctg cccgattacg acgcgctcac cgactgtatc cgctgcggcc
     8761 gctgcctgcc cgtgtgcccc acctaccagg agacggcgct cgagacgcaa tccccgcgcg
     8821 ggcggctcaa cctgctgcgc gccgtcgagg acggcaagat cgcgctcgac cacgctcgcg
     8881 tcgaagagca cttgtaccac tgtctcgact gccgcgcctg caacatggtg tgcccggcgg
     8941 gcatccccat cggcgagctg atcgtccagg ggcgcgccgc cgtcgagcat gcgcatcgcc
     9001 gcaactggct catccgcttc gcgctgaacg tcgtcctcac gtcgccccgc cgcgccgaag
     9061 tcctcaccgc gccgctgcgg ctcatccaga agctcaaact cgatcagctt ggcgtggttc
     9121 tcttcggatg gctgccgaaa atcggcccgg cgctacgtga cctggttcac atggctccgg
     9181 ccatatccag acccattcga cctgagcttg cccgccgtcc aagcagcaaa ggccccgacc
     9241 acccgaccac tggactactc gactactcga ctactcgacc acccgactac tcgaccactc
     9301 gaccacccgg ccactcggct aaccggcgtg tcggcttttt cctgggctgt gtcatgaacg
     9361 tggcgtttgc cgatgccagc cgcgcctcgg tcgcgctgat gcagcgcttc ggctgcgaag
     9421 tcgtgacgcc ggcagatcaa gcctgctgtg gcgcgccgca cgacgatcag ggtatgaaaa
     9481 acagggcgag agccttcaca cggcgcaata tcgaggcgtt cgagcggctg ggcgatctgg
     9541 ccgcgatcgt cgccgactgc gccgcgtgca gcggcttcct gaaggagtac gcgcacgcac
     9601 tgggcgacga tcccgagtgg gccgaacggg cggagcgatt ctcgtcgaaa gtgcgcgaca
     9661 tcaccgagtg gctggacgag atcataccag acgatcggag tgcaggcttc agcctgcctg
     9721 tcggcactcc gcaccgggtt gtgacctatc acgaaccctg tcatcttgcc cacatgcagg
     9781 gcgtgcgccg ccagccgagg gcgctcctgg cgcgcgcgga gaacctcgac gtgcgcgaac
     9841 tgcccgacgc gacgcgctgc tgcggtagcg ccggcatcta caacctgacg cacccggcca
     9901 tgtcgaaggc gctcctcggg cgcaagatgg ccgacgttgc gtcgaccggg gccggcgttg
     9961 tcgttagcgc caatccggga tgtttgatac aattggaatg gggcaggcgg tggcataagc
    10021 tgccggttgc cgtgaagcat attactgagg tgattctcga aagcctggtg acacgtgaag
    10081 cgtgaagcgt gattcgtggc gccgaatttc cattgggata agtaccacgc atcacataat
    10141 cacgtaatca cctaatcacg tgatcacgta cctacaacga tactgaacta tggacgtatc
    10201 cgtcacacaa acccttcacc gcgagcaaac cctcgtcggc gtgcgcttta tgcctgtcgg
    10261 caagatctac cacttcgacg ccacacgatt tcccgatcta cgcgagggcg actgggtgat
    10321 cgtcaccacg tcgcgcgggc ggcagatggg cgaggtcgcc acgttcgacc tgcccaagca
    10381 cggcgacgcg gacggcccct tcaaggcaat cgaccggatc gccaccaacc gcgatctcgc
    10441 ggtccggcac tactgggcac agaaagagac cgaagcgatg gtcatcggcc gcgagaaagt
    10501 gaaggagctg gggctgccgg tcaaaatcgt gaaagccgaa tacgcgttcg atggatcgcg
    10561 gctggcgttc ctctacagcg ccgaggaaga gaagcccgag ctgggcacgc tgcagcagga
    10621 cctccagcgc tcgttcaagg ccaaggtcga gctgcgcctc gtcggcccgc gcgacacggc
    10681 caagattatc ggcggcagcg gcgcgtgcgg gctggaggtg cgctgctgct cggctttcct
    10741 caccgagttc agcccgatct cgatcaagat ggcgaaggag cagggcatct cgctcaaccc
    10801 gcaggaaatc accggcatgt gcggccggct gcgctgctgc ctgatttacg agtacgaaca
    10861 atacgtggat gcgcgcagaa cgctgcccag gcgcgggaaa gaggtcggca cgccgcacgg
    10921 caccggcaag gtcgtcgacc tcttgccgtt gaaagagtcg gcgctcgtgc agatcggcga
    10981 ggcgaccttc gtggtgcacc gcgaagacat ccagccgctg gccgagctgg aggcgtttca
    11041 gaagaaggca gcctcgccgt gcggcgaagg cacgggctgc acgtgcggcc acgcgaagaa
    11101 gaagaaacgc aagagtcaat cgtcaggctc gggcgatggc ggcgacggcg gcgctgaatg
    11161 agttgcgggc ggcgatcggc gccgagcgcg tctcgaccga tccggctgaa ctgatcgtgt
    11221 atgcgtacga cggcgcgtgg gtggagggcc gccccgccgt cgttttgcat cccgaaacga
    11281 cccggcacgt cgccgagagt ctgcgcattg ccgaccgcga gcggatcccc gtcgtgccgc
    11341 gcggcgcggc gaccggattg gcgggtggcg cggtggcggt cgaaggcggc tggtgcctga
    11401 acctggcgcg gatgaacaag atactcgaca tctccgccgc cgattcggtc gccgtcgtgc
    11461 agccgggcgt cgtgacggca gcgctgcaga aggaagtcga aaagcgcggc ctgttctatc
    11521 cccccgatcc ggcgtcgctg cacatgtgca cgatcggcgg gaacgtgagc acaaacgcgg
    11581 gaggaccgcg ctgcctgaag tacggcgtca ccgccgatta cgtcctggcg ctcgaagtcg
    11641 tgctgcccgg cgggcgcgtc atgcgcaccg gccgccgcac gatcaaggac gtcgccggct
    11701 acaacctgac gcagttgttc gtcggctcgg agggcacgct cggcgccatc accgagatca
    11761 cgctgcaact gatcccgaag cccgctgccg aggccactgc gctggcggct tttccgcgca
    11821 tcgccgatgc gtgccaggca gtcgggagaa tcctggcttc gggcatcgtg ccgctggtga
    11881 ccgagctaat ggaccgcatc gtcgtgcgcg ccgtcgacgc atactatcgg agccgttctg
    11941 ccagtgccgg gctgccgctt gacgacgacg ccgtgctgct ggtcgcggtc gacggcgacg
    12001 gcgaccaggt cgagcgcgac atcgacacga tcgccgggct gctgcgtgga tccggcgcga
    12061 gcgaggtgcg ccgagcgcgg gacgcggtcg aggccgaagc gctgtgggaa gcgcggcgcg
    12121 cgatcaaccc ggcgctgatc cggctggcgc acgaccggct cggcgaagac atcgtcgtgc
    12181 cgcgggggca tatcccgcgc atggtcgagc gccttcggcg cattgccgcc gagcacgacc
    12241 tgacggtcgc gctgttcggc cacatcggcg acggcaacct gcatccgacg attctgtgcg
    12301 atcgccgtga cggcgagatg atgaagcgcg tccagtccgc ctccggcgcg atattcggcg
    12361 cagcgctcga tctgggtggc tccatcacgg gcgagcacgg catcggaatc gtcaagctcg
    12421 attatctgca gcgcgccgtc gatccgatcg cgctcgaagc gatgcaggcg atcaagcgca
    12481 tcttcgatcc gaacggtatc atgaacccgg gcaagaagct gccgagtgga gtaggggagt
    12541 ggtgacacag gcccaactga gatagtgttt cgctgtaatc gcaagctata cgaaggatgg
    12601 ttggcaatgc gcgaactaac cgagaaggcg cgagcgccaa gaaacagaac aattaccctt
    12661 tcgaccgatg aggccacttt ctaccagaag agactgctca aactctccag atcggctagt
    12721 gccgtggatc tggagaacaa gactattaac caggaccttt atgaggttct cgattggttg
    12781 cctgcttcct ttgtcgatct cctgtttatc gatcctccct ataatttgac gaagtccttt
    12841 aataccactg cgttcaacga gcgctcgacc gttgagtatc gagattggtt tgaatcatgg
    12901 tttccgaaac tgttgaagac actcaagccg agtgcttcgg tatatatttg cggcgactgg
    12961 cggtcttcca cggcgattca tcttgtcgca gaaaaatact tgatcgtcag gaaccgaatc
    13021 acgtgggagc gcgaaaaagg caggggagca agggcgaact ggaaaaactg ttccgaagat
    13081 atctggttct gcaccgtgtc agagacgtac acatataacc tggatgcagt gaaactcaag
    13141 cggcgtgtga tggcgccgta cacggattcg gaaggcaacc caaaagattg ggacaagacc
    13201 gaaagtggaa attatcgtct cacacatcca tcaaacgtgt ggacagacct gacggtgccc
    13261 ttttggtcga tgcccgagaa tacagatcat ccgacacaaa aaccggagaa acttgtggcg
    13321 aaaatcatct taagcagctc caaaccgggt gacttagtgt tcgacccgtt tctaggttcg
    13381 ggaacaacct ctgtcgtggc gaagaaactt agtcgaaggt atgtaggcat cgaaattgac
    13441 ccagcatact gctgtctggc agagaaacgg cttgcgatgg ccgaatacga aaagagcata
    13501 cagggttacg ccgaaggagt gttctgggag cggaactcgc tgcaggatcg caggtggagt
    13561 ttaagaaaga ggggtgggca gagtgtcttg ccgctctttg ctcaaggtct cgacaaggaa
    13621 cacgaatgag caaaagtcgg attttccaca ccggcagata taaggcgatc caaaaagcga
    13681 ttgtcaagct tctgaattca cagccggatt ttgtctcgac gcggacggcg gcaagcactc
    13741 gagctgtagg cgatgcgatt caaacgatcc tctctgaacg cttccagtca cttcttggtg
    13801 agttgtgcgc cgagtattcg gccgattttg ctcgccgggc gatggctgat ttggcgttta
    13861 ccgacaggga tggcctttac cacgtcgttg atgtaaagac gcataggctt ggaacgcagt
    13921 tcaatatgcc aaacttgacc tcggtcgaga ggcttgctcg attttacgag ggtgacgaaa
    13981 actacttcgt ttgtgttgtt cgtgaaatac gctatcgaag ggacgaaggt caagaccgaa
    14041 cgggcgcatt ttgttccgat cgagtttctc tcctgggatt gcctgacgat tggagcgctg
    14101 ggatggggac agatacagat agccaacgcg aatgttatca atatcaacga aggctattct
    14161 cgcaagaaat ggatgattga gttgtgcaac gtcatgctcg agttttatcc gaaggagatt
    14221 gggaagatcc atgagcggat agagtatttc agacgagtca agcggcgctg ggaaaagaag
    14281 caagaaagtt aggccgacag gcgatgatca tgggagggta ttttggccga cgagggactg
    14341 agagttctga atcgcgtgat ggtcattctg gcgcacccag atgaccccga attcttctgc
    14401 ggcgcgacga tcgcgaagtg ggcgaaagag ggcaggcaca tccgctattg catgctcacc
    14461 tgcggcgaca agggcagcga cgatccggat atggcgcacg atcgattgtg cgccattcgc
    14521 cacgaggagc agacgaacgc ggcgcgcgtt ctgggcgtga ccgacctggt gtggctgaat
    14581 tatcccgacg gcgagctggt gccgacgctc gaaacgcggc tgcgtgtcgc gcgcgagatt
    14641 cgcaaattca agcccgacgt cgtcgtgacg tgcgacccga cgcgcgtcat caccgcgttc
    14701 ggcacggtca accatcctga ccaccgcgcc gccggccaga tcgcgctgga cgccatcatg
    14761 cccggcgcgg gcaaccgcat gtacttcacg gagctgctgt ccgagggtct ggagccgcat
    14821 caagtgaaag aggtctacat cgcaggcgcg ctcgaacccg acacggcgat cgacgtgacc
    14881 gacaccttcg acgtcaagct gcgcgcgctt cgcgagcaca agagccagat caaggacccg
    14941 gatggcctgg agcagcgcat gcgggcgaga ttcagtcgag acggaatctt tgtcgagatg
    15001 ttcaagcggc tggtattggc ctgacgaatg atcagtgatc agttggcagt aatcagtaat
    15061 cactggctac tgatcactgg tcactgatta ctgattccta tgaggagcga atggccgacc
    15121 ttctactgct ggggacgggc gctgcgctga acgacggaag ccgcgagccg acgatgctgg
    15181 cgctgcgggg agccagctcg acggtactga tcgactgcgg ctcgaatccg gtgcgccagt
    15241 tgcagcgcct gggcgtgccg ctggcgtcga tcgagcgcct gatcttaacg cattcacatc
    15301 ccgatcacac ttccggcttt gcactcctgg tcgagatgct gtggctggcc gggcggcgcg
    15361 agccgctgcc cgtgcacggc ccggccgagg cgatcgacgt cgtccgccgc gtcttcgcgc
    15421 aatgggacac gagcgcgtgg aacgggctgc ccgatctgca atggcatgag gcacgcctgg
    15481 agatcggcgc gccgattgcg gccggcgccg acttcgagct gacggccgcg cctggaaagc
    15541 acagcgtgcc ggtcatcggc gtgcgcgccc gcgacatgca cggcggcggc gcactggcct
    15601 acggcgcgga cggcgagccg tcgcccggca ttcgcgcgct ggcgcagggc gccgatttgc
    15661 tcgtgcacga agcgaccggc gcatatccca cccacagcac cgccgaaggc gcggccggac
    15721 aggcgagcgc ggcgggtgtg aagcgcctgg tgctcgtgca tctatcgccg gccgtgaacg
    15781 acctggagac ccagcggctc gccgcaaaca agatattcgg cggcgaggtc ttcgtcggcc
    15841 gcgatctgga tcgctatgcg ttttagccat attgccggcc cgtccaattt cgaataatgg
    15901 cgagacttcg gaagtctcaa attgccatct cgcctgcgca atgacttgga tgccgggtat
    15961 cactgccggc gagaatgggc cagaacactt ccgaagtctg aaaggggcgt catgtcaacc
    16021 atcgacttca aacccgaggc gctcgcgatg aaagagcagc tcgtcgcctg gcggcgcgac
    16081 ttccaccggc atcctgagct ggcctaccag gaggtgcgca cgtcgggtat cgtcgcgaag
    16141 catctcggcg atctcggcct ggaagtgcag actggcgtcg gcaagaccgg cgtgataggc
    16201 gtgctcgacg gcgcgcagcc cggcccggtc gtcatgctgc gcttcgacat ggacgcgctg
    16261 ccgatccacg aagcgaaccg caccgactac gtgtcgcaga acccgggtgt gatgcacgcc
    16321 tgcggccacg acgcccacac cgccatcggc atgggcatcg cccaactgct gacgaagcat
    16381 cgcgatcggc tgtgtggcgc agtcaagttc gtgttccagc ccgccgaaga aggcggcaac
    16441 ggcgcgctgg ctatgatcaa cgacggcgcg ctggaaggcc cgcgcgcggc ggtgtcgatg
    16501 ggcttgcacg tgtggaacga aatgccggtc ggccaggcgg gtgtcggcgc ggggccgatc
    16561 atggcggcgg ccggcatcct caagatcaag gtgcagggca agggcgggca cggcgcgcag
    16621 ccggacaagg cgatcgacgc gatcctggcc ggctcggcca tcgtgaatgc actgcagacg
    16681 atcgtggcgc gcaacgtcga gccgcgccag acggccgtcg tcagcgtgtg cagtttccac
    16741 gccggcgacg cctttaacgt catcgccgac agcgccgagc tgatcggcac gtaccgctcg
    16801 ttcgacgacg gcgtgcagga actgatcatg tgccgcatac gcgaggtggc ggaggacact
    16861 gcgcgggcga tgggcgcgat ggtgaccgtg gagctgaggc cgatcacgtc gacgacggtc
    16921 aacgatgcac gcgtcgccga attcgtgcgc gccgacgcga gcgacgtgct cggcgcggat
    16981 cgcgtcgtgc ccgagcagtt caccatgggc gcggaagaca tgagcgagtt cctcaagcgc
    17041 gtgccgggct gcttcttctt cctcggctcg cgcaacgacg agaggggctt caatgcgccg
    17101 caccacaacc cgcgcttcga catcgacgag aacgtgatgc cgctcggcgt cgcgattctc
    17161 gcgcagtcgg cgacgaggta tctatcgggt caggggaagg tgggaactcc ttcggtgccg
    17221 tgagttcccg ttgtctcatg aggaactgaa ggaactcaat ttgcgcgatc aaggcgatga
    17281 ctcaagcagg ccctctgcgc tggcgaacgg gaaatgggtg gctggtgctc gtcggcggcg
    17341 gacgatggaa tacgaccgag gcaatcgacc gccgcgcgat tgacgcgatg acggacgagg
    17401 cgccgatcgc gttcgtcccg gcggcgggct gcccgccgga ctacggcgag tcgttcctgg
    17461 cgcactacgc gcgcctgggc gcgcccgccg gctacgtcgt gcccatacac gatcaggcat
    17521 ccgcaaacga tccggccaac gtgcgccggc tgacgcacgc cgggctggtg tacttcggcg
    17581 gcggcgacat gcggcagttg ctcgacgcca tgaccggcgc gcaggcgctc gacgccgtcg
    17641 cggcggcgta tgcggccggc gccgtcatcg tcggcatgag cgccggagca gccgcgctgg
    17701 ccgcctgggg cttatcgtcc gatccgggcg ttggcctgct caggggctgg ggctggctgc
    17761 cgaatgcgat cgtcgcgcca cattacgctc ccgcgcaggc cgatgcgctg cacgccgggc
    17821 tgcgcgagca tccgcagcta cttgggctgg gcatcccgga ggacgtggcg ctcgcgctag
    17881 gtcctgacgc cgaagtcttc acctggggac gtggacagat tactgtcacg ctcggttcga
    17941 agtttgacgc agaaggatga aggatgaggg atgaaggatg aatctggcga ccaggtccga
    18001 gacgttcgtg gtcgtgtatt cagcctgcct tgtcctcccc ggcggatcgt ctcgcttgtg
    18061 ccgtcgatca cc
//
LOCUS       DBNHXB010000215        12902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_720101, whole genome shotgun sequence.
ACCESSION   DBNHXB010000215 DBNHXB010000000
VERSION     DBNHXB010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 12902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12902
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_720101"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="AC162G_08930"
     CDS             complement(<1..257)
                     /locus_tag="AC162G_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075977.1"
                     /translation="MKRSGGYPESALTASRQEAQLSGGFLCTKPIDPFSTQGAAGPLT
                     RYSGRIITEENLMSENELLTKALTDTILTRRSFLKWSGVLGG"
     gene            complement(304..972)
                     /locus_tag="AC162G_08935"
     CDS             complement(304..972)
                     /locus_tag="AC162G_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5075978.1"
                     /translation="MLPLRVLVADDHRLFRQGLIGLMNTRKDLVEVVGEAASGREAVL
                     LAQRLQPDVVLMDIHMPDGDGLQATARIRQLSSQTSVVMLTASELDEHLYEAVRLGAA
                     GYLLKSLDASELFDLLDGVSRGEAAMTRAMAARVLKSVAGHATHVDGGREPLTEREID
                     VLRLVAQGMSNPEIAERLNITVNTVKVHLRNILDKLQVENRTQAAAYAVQSGLVTSLL
                     PDPA"
     gene            complement(988..2937)
                     /locus_tag="AC162G_08940"
     CDS             complement(988..2937)
                     /locus_tag="AC162G_08940"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR5075979.1"
                     /translation="MTAVFFVYGLAFFCLGLAVALESRRTSDLPLGRQLPWLAAFGFA
                     HSLVEWADMFLLLNPTGLPHDVLTLARTLLLPLSAVLLVRFGIGLVGEAGPLPVWMTF
                     LPVVLLVPIALMVGYAVVVAFTQSSVEKAADVWSRYLLYLPGNLLAGFGFVRQARVLW
                     HDASAPLSTSLDVARRLLLGAALAFALNAVVAGLIVPAAPYGLAPWLNYDTVQSITGV
                     PVQIWRMVSAVAVTIFVVRALDVFEAERAQRLAALDKARAQAQHAALQAQSEARQIAE
                     GWTNGLVSISRCIANLENVDTILSAIVTLSQRLLKSDTAALALWDETGTRLVMKCHAT
                     VDQTQAPPCPEVCSPLIVEPLRAGRAACLSEHGQDGESRWRCPAADQEIQTTAIVPLQ
                     LDGRPVGGLWAGRFTPEPFTTADLIGLERLADQAVIAIEHALMAARLQSLAVTEERGR
                     IAREMHDGLAQVLGFLSLETQTLEVLVRQGNCEAALAELEQARKRINEAQANVRENIL
                     SLRTTLAGEVGLMPALRQYVEEFGVQTGIDARLVSEVEEALPLSPIAETQLVRIVQEA
                     LANVRKHAQAHHAQVRLAARSGCLCVTVADDGIGFDGQAVRSHFGLQTMRERAQSVGG
                     GLTIASQPGEGTQVELWLPLLPQRN"
     gene            complement(2971..3753)
                     /locus_tag="AC162G_08945"
     CDS             complement(2971..3753)
                     /locus_tag="AC162G_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorD/DmsD family molecular chaperone"
                     /protein_id="HIR5075980.1"
                     /translation="MNNLSAASLSRQESPSTVDPRRRAFDILSRVAADRGRVYRWLAI
                     GFYPPDASLIAALNTGHMVDEIQASIAWLGKDQAKLQAGLSALQRFVDATLADLEADY
                     ARLFGKSVERVSARESAYRWRDASDVLSATETIAQALRQQYTQFNTVPMNGYEDHVAV
                     ELEFLAFLCEREAASWASGAQERARQLRRQERVFLDDHLGRWLAEFCWRVCDRLPDTF
                     YAALADLGDVWLSLEHGPGYLPAAASNQLPVISGQSSVASNQ"
     gene            complement(3753..4373)
                     /locus_tag="AC162G_08950"
     CDS             complement(3753..4373)
                     /locus_tag="AC162G_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5075981.1"
                     /translation="MSERYVMVVDLQACTGCHTCTVACKQENNLPEGVAWIQIVSIGS
                     DGDEAPAGDYPDLQLEYLPLACQHCAGGPCVEVCPTSATARSANGLVTLDPSLCIGCR
                     YCMIVCPYTSVRVFADSQLRYALPYPTGSNPMIHRAKTVEKCAFCAHRLVQELQPACV
                     EACPTRALIFGDLNDPNSDVAQLLRARPHFQLLVEKGTEPSVYYLT"
     gene            complement(4366..7236)
                     /locus_tag="AC162G_08955"
     CDS             complement(4366..7236)
                     /locus_tag="AC162G_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5075982.1"
                     /translation="MDAHRPPQIVVAVSHSWLRNTFSQLLQRLPVDILPVENVDDLLI
                     RARAKPDAIVLDPFAFGRSSLDLVTRIRREAHYVPVIVLLSSDTGDYRDAAMRLGANA
                     AIAVERAATDLLPTLKTLLGRANLVNGIADRIARTARSNAPAGEAAYGSDPATLNLRE
                     RSERTVEQRAMVPETLAAIEPPAGDVATSLKDDGRVFLRGLSLSAGSPHGSSPGRQYR
                     TACNLNCGAHYCGLAVTVHDGRITRIEPADFPDERYRRICLKGISHVQMVAHRDRLLY
                     PLKRAGPRGTSEWERVSWDQALDEIADRVRALREKFGAESLMFFPFSGQLSALNGLTG
                     VYLRLAAALGASGTSLREFGLDSAVPSGIEDTLGEGAGYLANDYADLPNSRLVLIWGG
                     DPAQSRMNWWPFFVEAQRAGTHLIAIDPKYSVTAGKCDEWLPIRPGTDLYLALAMLRE
                     IIARDWIDREFVIRHTVAPLLVREDDGRFLNGYLVWDEATQRAVPAERAKSPALAGRF
                     VVKGVACRPAFDLLKERVSAYTPDFAAQKTGLPAERIIALAQAYATVKPARIFTLYGV
                     DRWHHGATFGRLIATLAALTGNLGVPGGDAGVDGFCEGALFDSAFCAPGGKLHRPVNP
                     LRMPEQIVTGLPYPIKAMWVAFGNWLNQWPDQNRLRQEVLPKLDLLVTVDHFMTETAR
                     WSDYVLPAAMLFEREDMVKGPGPFVQYQPVIVPPPGECRSDFDIAAALAGLLGVREYF
                     SRQPVEYLAEILAQGDASMRRLSFEELKSKGVLQRDLPPDALVVHRDRKFKTPTGRVE
                     FYVERLLPYGQALPNYQPPIEADPDGDLIHRYPLICLTEHSRYRVHSTFVNTPWLREL
                     DREPCAILHPSVAQPRRIANGDRVRLFNDRGFVVLRARLSQSVPPGTVYLSQGWQSTD
                     FQSGHAQALTHAVGNPANAFGPNCSFSDVLVEVVREVGRQDV"
     gene            complement(7366..8010)
                     /locus_tag="AC162G_08960"
     CDS             complement(7366..8010)
                     /locus_tag="AC162G_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5075983.1"
                     /translation="MSEATRVLIVDDHAVVRSGLRLLLEKESDIRVVGEAEDGARAVR
                     QALDLRPDVVLMDITLPDFDGVEVTRRIRAAWAEARVLALTMHSEETHLVPFIEAGGM
                     GYVRKSAADRDLVTAIRTVARGESFLQPEGVQAIVQERRSAKSGPPAPDVLSERERQV
                     LELTARGFTSREIGERLSLSSRTVETYRERIMEKLGLEHRSQLVEYALRHRLLG"
     gene            complement(8003..9172)
                     /locus_tag="AC162G_08965"
     CDS             complement(8003..9172)
                     /locus_tag="AC162G_08965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR5075984.1"
                     /translation="MILLSIKRIASHAWRRLGAAPVAIKIVGLVVLPLLLVLVAGALF
                     VRQRLLILLAAPEHHSANFDLVFTLTREGSAVLVLGALIGLGLAVLLTWVLVRPLRNL
                     VHAMRRVQAGDLSVEVSAWAHDEIGEVQAEFNAMVAGLRESREALMRQQGELEALNHE
                     NTRLLAELHAKSERLQQLLNHALSAQEAERRRLSRELHDETGQALTSILVQLKALQDE
                     TDLDLIQDRLNGLRYLTGQTLEEVRRLSMDLRPAVLDDLGLIPAIRWCVKECAEQKGT
                     EVNFNTTDSLGRLPSEVEIVLYRAVQEGLTNIVRHAQAQRAEINLARGPRAVWLTIID
                     DGRGFESNDRNGKGLGLAGMQERVALIGGRLQVDSEAGGGTRIFIEIPLEEAQRE"
     gene            complement(9247..12276)
                     /locus_tag="AC162G_08970"
     CDS             complement(9247..12276)
                     /locus_tag="AC162G_08970"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5075985.1"
                     /translation="MTPKPSMPIELRLLGPVQVIWEKGKAPRFRSPKAIALLGYLAAE
                     ARPISRDHLIGLFWPDASDDQARSELRRVLHNLSTILPGCLETDRRTIHFQPAPAVDV
                     DIVTFRELHAKGDAASLAAAAELYRGEFMEGVALADCPEFETWLITERERWRQAAWRV
                     LDALITLHAHHGEFDQALRFASRLVALDPWREEAHRRLMLMLARTGQRDAALRQYGTC
                     RRVLAEELGVEPTTETTALYERIKAAASRSPNLPIQPTPFVGRQPELADLARLLADPH
                     CRLLTIVGPGGVGKTRLATEVAACTVNAFLNGISYVSLTPVMSAEFLITAVAEALGLR
                     FSGAASPRTQLLDHLRARELLLLLDNFEHLMAGVDLVADILRQAPEVKVLVTSREKLN
                     LREEWLFELDGLPYPSAGAGDGAALAGYDAVQLFTHNARRARHQFDVHSEIDSVARIC
                     RLLTGSPLGLELAAALVATQSCTQIADDIARSRDALSATWRNMPERHRSLRAVFDHSW
                     RLLTLDEQRVFRRLSTFRGGFTLAAARQVAGLAPSMLNALVAKSLLQRMGDDADSPRY
                     DIHEILRQYAAEMLAAEPSEREQTSTQHAGYYAVFLQEQEQRFNTSTVGESLAAIRRE
                     IDNVRAAWQRLVARRDLERIGCVIATLHKFYEAQNWFQEGSDQLDQARAAIAPHADSL
                     SMAERVIWGRLHAHEAGLLLRLGQVSRMRALAEAAVSLLRGHAGTDTLGFALNVAGAA
                     LIQAGEFVAARARLEEALALYRQAGNRKETVKVLANLGNVAGRAGDYAYSIETLGEGL
                     SLCREIDDRRGEGFFLNNLAAVHLMLGNLAEARTHFEACLPVCEETGNLYVKTVALQN
                     LGELCLRQADHSRALFFCEEAVALARRIDSVSQLARGLKWLALVQIERQEHATAWGHL
                     REGLQAAQASQSAMIILDMLEAAAVWRLRNGELDRAVDLLTLLVRHPATEQHTRENAT
                     RLLNELNVTPDAPADDESLQLEKVVQALLATPPTPAG"
     gene            12622..>12902
                     /locus_tag="AC162G_08975"
     CDS             12622..>12902
                     /locus_tag="AC162G_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075986.1"
                     /translation="MTRKTLVLVSGLAALTLVISLSAAAAQDAGPQTESSVQAASAMT
                     GYYNLFGYEGIGTLGSVGFAAGPTPPVGDYVTTHCRYIDVQNTTVGHYYL"
BASE COUNT         2375 a         4212 c         4057 g         2258 t
ORIGIN      
        1 ccgcccagca cgccgctcca cttcaggaaa ctgcgccggg tgaggatggt gtcggtcagg
       61 gctttcgtga ggagttcgtt ctcactcatg agattttcct ccgtgatgat tctgccggaa
      121 taccgggtaa gagggccggc agcgccctgc gttgagaaag ggtcgatcgg tttcgtgcat
      181 aaaaagcctc ctgagagttg agcctcctgt cgggacgcag taagggcact ctcaggatag
      241 ccgcccgatc gtttcaacgc atcactccac ggaataatcc ggcatcgccc ggtcgggtga
      301 tatttacgcg ggatccggca aaagtgaggt caccagtcct gactgaacgg cgtaggccgc
      361 tgcctgtgtc cgattctcga cttgcagctt gtcgaggata tttcgtagat gaaccttgac
      421 ggtattcacc gtgatgttga gccgctcggc aatctccgga ttgctcatgc cctgtgccac
      481 gagccgcagc acgtcgatct cgcgctcggt gagcggctcc ctaccgccat caacgtgcgt
      541 tgcgtgtccc gccacgctct tcagcacccg tgccgccatc gcgcgtgtca tggccgcttc
      601 accgcgtgat acgccgtcga gcaaatcgaa caactcggac gcatccagac tcttgagcag
      661 gtaaccggcc gcgcccaggc gcacagcctc gtacaggtgc tcgtccagtt ccgaggccgt
      721 cagcatcacg acagaagttt gggacgagag ctgtcggata cgagccgtcg cctgaagacc
      781 gtccccatcc ggcatatgaa tgtccatcag cactacatcc ggttgaagac gctgcgccag
      841 cagcacggct tcacggccgg acgctgcttc acccaccacc tcgaccaaat ccttgcgggt
      901 gttcatcaag ccgataagcc cctgccgaaa caggcggtga tcgtctgcga ccaaaaccct
      961 gagcggaagc atcgctacct cctcgtctca attacgctgc ggcaacagcg gcagccatag
     1021 ttcgacttgt gtcccttcac cgggctgcga cgcgatcgtc aacccaccgc cgacgctttg
     1081 tgcgcgctcg cgcatagtct gtagtccgaa gtggcttcgg acggcctgcc catcgaaacc
     1141 gataccgtcg tcggcgacgg tgacgcacag gcagccgctg cgcgcggcca gccgaacctg
     1201 cgcatgatgc gcctgtgcgt gcttgcgcac gttcgccagt gcctcctgga cgatgcgcac
     1261 gagctgcgtc tcggctatgg gcgacagtgg cagcgcctcc tcgacttcgc tcaccagccg
     1321 ggcatcgatg ccggtctgca cgccgaactc ttcgacatac tgtcgcagcg cgggcatcag
     1381 cccgacttcg ccggccagtg tcgtgcgcag gctgaggatg ttctcgcgca cattggcctg
     1441 cgcctcgtta atgcgcttgc gcgcctgctc caactcggcc agcgcggctt cgcagttgcc
     1501 ctggcgcaca agcacttcta gcgtctgcgt ttccagactc aggaatccca gcacctgcgc
     1561 cagcccatca tgcatctcgc gcgcgatgcg cccgcgctct tctgtcacgg ccagtgattg
     1621 aagacgggcc gccatcaacg catgctcgat cgcgatcacg gcctggtcgg ccagccgctc
     1681 caatccgatc agatcggcag tcgtgaaagg ctctggcgtg aaccgcccgg cccacagccc
     1741 gccgaccggc cggccgtcca attgcagcgg aacgatggcg gtggtctgaa tctcctgatc
     1801 ggctgccggg cagcgccatc gagattcacc gtcttgcccg tgttcggaga ggcaggctgc
     1861 gcggccggcg cgcagcggct cgacgatgag cgggctgcac acttccggac agggtggcgc
     1921 ctgagtctgg tcaaccgtgg catggcactt catcaccagg cgagtcccgg tctcgtccca
     1981 tagcgccaga gcagcagtat cggatttcaa caggcgctgc gagagtgtga cgatcgccga
     2041 caggatcgtg tccacgtttt ccagattcgc aatacaccgg ctgatactca ccagtccgtt
     2101 tgtccaaccc tcggcaatct gccgtgcctc ggactgagcc tgcaatgccg cgtgctgcgc
     2161 ctgcgcccgt gccttgtcca gcgcggccag ccgctgggca cgctcggcct cgaacacatc
     2221 cagcgcgcga acgacgaaga tcgtcacggc caccgccgac accatgcgcc agatctgcac
     2281 gggcacaccg gtgatcgatt gcaccgtgtc gtaattgagc cagggcgcga gaccatacgg
     2341 cgcggccggc acgatcaggc cggcgacgac ggcgttgagc gcaaacgcca gcgccgcgcc
     2401 cagcagcagg cggcgcgcca cgtccaggct tgtgctgagc ggagccgaag catcgtgcca
     2461 cagtacgcgt gcctggcgca cgaagccaaa accggccagc agattgcccg gcagatacag
     2521 caggtagcgc gaccacacat cggccgcctt ttcgaccgat gactgcgtga aggccacgac
     2581 aactgcatag ccgaccatca gcgcgatcgg caccagcagc acgaccggaa gaaacgtcat
     2641 ccacacaggc agcggaccgg cctcgccgac cagtccgatg ccaaaccgga caagcagcac
     2701 tgccgagagc gggagcagca gcgtgcgagc gagcgtcaac acatcgtgcg gcaatccggt
     2761 gggattgagc agcaggaaca tgtccgccca ctcgaccagg ctgtgcgcga agccgaaagc
     2821 cgccagccac ggcaactgtc tgccaagcgg gagatcgctc gtccggcgcg attccagcgc
     2881 gaccgccagc ccgaggcaaa agaaagccag cccgtagaca aaaaagaccg cagtcatgtc
     2941 ttcttcagct cagttatcag tcatcggtgc tcactgatta ctggccactg atgactggcc
     3001 actgattact ggtaactgat tactggccgc agcaggcagg tagccggggc cgtgctccag
     3061 gctcaaccag acgtcgccca ggtcggcgag cgcggcgtaa aacgtatcgg gcagccgatc
     3121 gcacacgcgc caacagaact cggccagcca gcggcccagg tgatcgtcca ggaacacacg
     3181 ctcctggcgg cgcaactgcc gcgccctctc ctgcgcgccc gatgcccaac tcgcggcttc
     3241 gcgttcgcac aggaaggcca ggaactccag ttcgacggcg acgtgatctt cgtagccgtt
     3301 catcggcacg gtgttgaact gcgtgtactg ctgccgcaac gcctgcgcga tagtctcggt
     3361 cgccgacagc acatccgatg cgtctcgcca gcgataggcc gattcgcgcg ctgagacgcg
     3421 ctcgacgctc ttgccgaaca gccgcgcata gtccgcttcg agatcggcca gcgtcgcgtc
     3481 gacgaatctt tgcagcgcgc tcagaccggc ctgaagtttg gcctgatcct tgcccagcca
     3541 ggcgatcgac gcctgaatct cgtcgaccat gtggcctgtg ttcagcgcgg cgatcaggct
     3601 agcatcgggc gggtagaagc cgatcgccag ccagcgatac acgcgccccc gatcggcggc
     3661 cacgcgcgac aggatatcga aagcacgccg gcgcggatcg actgtggacg gcgactcttg
     3721 tcgagagagg ctggctgcgg aaaggttatt catcatgtca ggtagtagac cgaaggctca
     3781 gtgcctttct caaccagcag ttgaaagtgc gggcgcgccc ggagcagttg cgccacgtca
     3841 ctgttgggat cgttcaggtc gccgaagatg agggcgcggg tcgggcacgc ctcgacgcag
     3901 gccggttgca gctcctgcac cagccggtgc gcgcagaacg cgcacttctc taccgtcttc
     3961 gcgcggtgga tcatcgggtt gctccccgtg gggtagggca atgcgtagcg cagttgcgaa
     4021 tcggcgaaca cgcgcacgct ggtatacggg cagacgatca tgcagtagcg gcagccgatg
     4081 cacaggctgg ggtctagcgt gacaagtcca ttcgcggagc gagccgtggc cgacgtgggg
     4141 cacacttcga cgcacggccc gccggcgcag tgctggcagg ccagtggcag gtattcgagt
     4201 tgcaggtcgg gataatctcc ggcgggggcc tcatcgccgt cggaaccgat gctgacgatc
     4261 tgaatccacg cgacgccttc gggaaggttg ttctcctgct tgcaggcaac cgtgcaggtg
     4321 tggcacccgg tgcacgcctg gagatcgacg accatcacgt aacgctcaga catcctgccg
     4381 ccctacttct ctgacgactt cgacgagaac atccgagaag gagcaattcg gcccaaaggc
     4441 gttggccgga ttgccgacgg catgtgtcag cgcctgtgca tgacccgact gaaaatcggt
     4501 cgattgccag ccctggctga gataaactgt ccctggtggc acagactggc tgagacgcgc
     4561 ccgcagcacg acgaagccgc gatcgttgaa tagccgcacc cgatcgccgt tggcgatccg
     4621 gcgaggctgg gcgaccgagg ggtgcaggat agcgcacggt tcccgatcca gttcgcgcag
     4681 ccagggcgtg ttgacgaacg tcgagtgcac ccggtagcga ctatgctcgg tcaggcagat
     4741 cagaggataa cggtggataa ggtcgccatc tggatcggct tcaatcggcg gttgatagtt
     4801 cggcagggcc tgaccatagg gcaacagccg ttcgacgtag aactcgaccc ggccggtcgg
     4861 cgtcttgaac ttccgatcgc gatgcaccac aagcgcatcg ggcggaaggt ctcgttgcag
     4921 gacgcctttc gatttcagtt cttcaaacga cagccggcgc atcgacgcgt caccctgcgc
     4981 cagaatctcg gccaggtatt cgaccggctg ccgggagaag tactctctga cgcctagaag
     5041 cccggccagc gccgccgcga tgtcaaagtc cgaacggcac tcgcccggtg gcggcacgat
     5101 gacgggctga tactggacga acggtcccgg ccctttgacc atgtcttcgc gctcgaacaa
     5161 catcgccgcc ggcagcacgt agtcagacca gcgcgccgtc tcggtcatga agtgatcgac
     5221 ggtcaccagc aaatcgagtt tgggcagcac ttcctggcgc agccgattct gatccggcca
     5281 ctggttcagc cagttgccga aggcgaccca catggccttg atggggtagg gcaggccggt
     5341 gacgatttgc tcgggcattc gcagagggtt caccggacga tggagtttgc cacccggcgc
     5401 gcagaaggcg ctgtcgaaga gagcgccttc gcagaagcca tccacgcccg catcaccacc
     5461 gggcacgccc aggttaccgg tcagcgcagc cagtgtcgcg attaaccggc cgaaggtcgc
     5521 gccgtgatgc cagcgatcga cgccatacaa cgtgaagatc cgcgccggct tgaccgtggc
     5581 atatgcctgc gccagcgcaa tgatccgctc cgcgggtaac cccgtcttct gcgcggcgaa
     5641 gtccggcgtg taagcggata ccctctcctt caacagatcg aaggccgggc ggcacgcgac
     5701 gcctttgacg acgaatcgac ccgcgagcgc aggagacttt gccctttcag caggaaccgc
     5761 gcgctgcgtg gcttcatccc agaccaggta gccgttgaga aagcgaccat catcctcgcg
     5821 caccaacagc ggcgcgacgg tgtgccgaat gacgaactcc ctgtcgatcc agtcccgcgc
     5881 gatgatctcg cgcagcatcg ccagcgccag atagagatcg gtgcctggtc gaatcggcag
     5941 ccactcgtcg cacttgcccg ccgtgacact gtacttcgga tcgatggcga tcaggtgcgt
     6001 tcccgcccgc tgcgcctcga cgaagaacgg ccaccagttc atgcggctct gcgccggatc
     6061 gccgccccag atcagcacca ggcgcgaatt gggcaggtcg gcgtagtcgt tggcgagata
     6121 gcccgcgcct tcgccgagcg tgtcttcgat gccgctgggc acggcgctat ccaggccgaa
     6181 ctcgcgcagg ctggtgcccg aagcgcccag cgcggcggcc aaccgcaggt acacgccggt
     6241 caggccgttc aacgcgctca actggccgga gaaggggaag aacatcaggc tctccgcccc
     6301 gaacttctcc cgcagcgccc gcacccgatc ggcgatctcg tccagcgcct gatcccagga
     6361 gactctttcc cattcgctgg tgccgcgcgg gccggcgcgt ttgagcgggt acagcaatcg
     6421 atctcgatgc gccaccattt gcacgtggct gatacccttc aggcaaatgc ggcggtagcg
     6481 ttcatccggg aaatcggccg gctcgattct ggtgatgcgt ccatcatgca ctgtcaccgc
     6541 caggccgcag tagtgcgcgc cacagttgag gttgcacgcc gtgcgatact gccgccccgg
     6601 cgaggaaccg tgcggcgagc cggccgagag cgacaggccg cgcaggaaga cgcggccgtc
     6661 gtctttgagt gaagttgcca cgtcgcctgc cggcggctca atcgcggcaa gcgtttcggg
     6721 gaccattgcg cgctgttcga ccgtgcgctc gctgcgctcc cgaaggttca acgtggccgg
     6781 gtcacttcca tacgccgctt cgccggcggg cgcgttgctg cgcgcggtcc gggcaatgcg
     6841 gtcggcgata ccattgacca ggttggcgcg tcccagcagc gtcttcaacg tcggcagcag
     6901 atcggtggcg gcccgctcga cagcgatggc ggcattggcg ccgaggcgca tagctgcatc
     6961 gcgatagtcg ccggtgtcgg aagacagcag cacgatgacc ggcacgtagt gggcttcgcg
     7021 ccgaatccgc gtgacgagat ccaggctaga cctgccaaag gcgaacggat cgagcacgat
     7081 cgcgtctggc ttcgctctgg cccggatcag caggtcgtct acgttctcaa ccggtagaat
     7141 gtcgaccggg agacgttgaa gcaactgtga aaaggtgttt cgcagccaag agtgagagac
     7201 ggcgaccacg atctgaggag gccgatgcgc atccatttgc gtgatccttc gatgatcgcg
     7261 ccatcgtcat tatgaagcaa cgggcgactc ctgtcagtcg ggagtcgccc gcgatttgcc
     7321 gcgtattttt ctcgacaagc gtgtggggat tcttacggac agagactatc ccagcagacg
     7381 atggcgcagc gcgtattcca ccaactgcga ccggtgttcc agccccagtt tctccatgat
     7441 acgctcgcga tacgtctcga cggtgcgtga agagagcgac agtcgctcgc cgatctcacg
     7501 gctggtgaac cctctggccg tcagttcaag tacctggcgc tcacgctcgg atagcacgtc
     7561 cggtgcaggt gggccggact tcgcggaccg gcgctcttga acgatcgcct gtacgccttc
     7621 gggctgcagg aaagattcgc cgcgcgcgac cgttcgaatg gcggtgacca gatcgcggtc
     7681 agccgcggat ttgcgcacat agcccatgcc gccggcctcg atgaagggaa cgagatgggt
     7741 ctcctcgctg tgcatggtca gcgccaacac gcgggcctca gcccaggccg cgcggatgcg
     7801 acgagtcact tcgacaccgt cgaagtcggg cagtgtgatg tccatcagca ccacgtcggg
     7861 ccgcagatcg agcgcctgcc gcactgcccg cgcgccgtct tcggcttcgc cgaccacgcg
     7921 gatgtcagat tctttttcga gcagcaggcg gagaccgctg cgcacgacgg cgtgatcgtc
     7981 aacgatcaga acgcgagtgg cctcactcac gctgcgcctc ctccagcgga atctcgatga
     8041 agatgcgcgt gcccccgccg gcttccgagt ccacctggag ccgcccgccg atcaacgcga
     8101 cgcgctcctg catcccggcc agtcccagcc ccttgccgtt acggtcgttc gattcgaacc
     8161 cgcgcccgtc gtcgatgatg gtgagccaga ccgcgcgagg cccacgcgcg aggttgatct
     8221 cggctcgttg cgcctgcgcg tgccgcacga tattggtcag cccctcctgc accgcgcgat
     8281 agagcacgat ctcaacttcg gaagggagac gtcccaacga gtcggtggta ttgaagttga
     8341 cctccgtccc cttctgctcg gcacactctt tgacgcacca ccggatggcc gggataaggc
     8401 ccagatcatc gagcacggcc gggcgcaagt ccatggacag tcggcgcacc tcttcgagcg
     8461 tctggccagt cagatagcgc aggccgttca ggcgatcctg gattagatcg agatcggttt
     8521 cgtcctggag ggctttcaat tgaaccagga tcgaagtgag cgcctgcccg gtctcatcgt
     8581 gcagctcgcg cgagaggcgc ctgcgctcgg cttcctgcgc cgaaagcgca tggttgagca
     8641 attgctgaag tcgctcgctt ttcgcgtgca gttcagccag caggcgtgta ttctcgtgat
     8701 tgagggcttc cagctcgccc tgctgccgca tgagggcctc gcgcgattcg cgcagtccgg
     8761 ccaccatcgc attgaactcg gcctgcacct cgccgatctc gtcgtgcgcc caggctgata
     8821 cctcgacgga caggtcgccc gcctgcacgc gtcgcatggc atgaaccagg ttgcgcaacg
     8881 ggcggacgag cacccaggtc agaaggacag ccagtccaag cccaatgagc gcaccgagca
     8941 cgaggacggc gctgccctcg cgcgtcagcg tgaagacgag atcgaaattg gctgaatgat
     9001 gctcaggcgc agccaaaagg atgagcaggc gctgccgaac gaacagtgct cccgcgacca
     9061 gaacgagaag cagcggcagg acgacgaggc cgacaatctt gatcgcaacc ggggctgcgc
     9121 cgagtcggcg ccaggcatga gaggcaattc gttttatgct gagaagaatc atcggaatgg
     9181 tcccaccggg taccgatagg cgaacggggt agcagcataa ttataaacgc aagtggctta
     9241 ccccgttcat cctgcaggcg ttggtggagt agccagcaag gcctggacga ccttttccag
     9301 ctgtagactc tcgtcgtctg ccggagcatc cggcgtcacg ttcaattcgt tgagcaggcg
     9361 cgtggcgttc tcccttgtgt gctgctccgt cgccggatgt cgcaccagca gcgttagcag
     9421 atcgacggct cgatccagtt cgccgtttcg cagccgccac acggctgccg cttcgagcat
     9481 gtcgagtatg atcatcgccg actggctggc ctgtgccgcc tgtagtcctt cgcgcaaatg
     9541 cccccaggcc gtcgcgtgct cctgtcgctc aatctgaacg agggccagcc atttcaagcc
     9601 gcgcgccaac tgactgacgc tgtcgatccg gcgcgccagc gccacggcct cctcgcagaa
     9661 gaacagcgcc cggctgtgat cggcctgcct caggcacagc tcgcccagat tctgcagcgc
     9721 gactgtcttg acgtacaggt tgccggtttc ctcgcagacc ggcagacacg cctcgaagtg
     9781 agttcttgcc tccgccagat tgcccagcat gagatgcacc gctgccaggt tattcaggaa
     9841 aaacccttcg ccgcgccggt cgtcgatctc gcgacagagg gaaagcccct cgccgagcgt
     9901 ttcgatggaa taggcatagt cgccagcgcg cccggcgacg ttgcccaggt tcgccagaac
     9961 cttcaccgtt tccttgcggt tgcctgcctg ccgatacagc gccagcgcct cttccaggcg
    10021 tgcccgcgcc gcgacgaact cgcccgcctg gatcagtgcc gcgcccgcga cgttgagcgc
    10081 gaagcccagc gtgtccgtgc cggcatgccc gcgcaacagg gacacagccg cctcggccag
    10141 tgcgcgcatg cgtgagactt gccccagtct caacagcagg cccgcctcgt gcgcgtgtaa
    10201 gcgtccccat atgacgcgct ccgccatcga cagcgaatcg gcgtgcggcg cgatcgcggc
    10261 gcgggcttgg tcgagctgat cgctgccttc ctgaaaccag ttctgtgctt cgtaaaactt
    10321 gtgcagcgtt gcgatgacgc agcctatcct ctccagatcg cggcgcgcta ccagtcgctg
    10381 ccacgccgcg cggacgttgt cgatctcgcg ccggatcgcg gccagggatt cgccgactgt
    10441 ggatgtgttg aagcgctgct cctgctcctg aagaaagacc gcgtagtaac cggcgtgctg
    10501 cgtcgatgtt tgctcgcgct cggacggttc ggcggcgagc atctcggcgg cgtactggcg
    10561 caggatttcg tggatgtcgt aacgcggcga gtcggcatcg tcgcccattc gttgtagcag
    10621 cgacttggcg accagcgcgt tgagcatcga aggcgccagc ccggcgacct gccgcgccgc
    10681 agcgagggtg aacccgcccc gaaacgtgga cagccggcga aacacccgct gttcatcaag
    10741 cgtgagcaac cgccacgagt ggtcgaacac ggcgcgcagg ctgcgatggc gctccggcat
    10801 gttgcgccac gtggccgaca gcgcatcccg gctgcgcgcg atgtcgtcgg cgatctgtgt
    10861 gcacgattgc gtcgccacca gcgcggcggc caattccaga ccgagcggcg agccggtcag
    10921 caggcggcaa atgcgcgcga cgctgtcgat ttcgctgtgg acgtcgaact ggtggcgcgc
    10981 gcgccgcgcg ttgtgcgtga agagctgcac cgcgtcgtaa ccggcgagcg ccgcgccgtc
    11041 gcccgcgccg gcggacgggt agggcagccc gtcgagttcg aacaaccatt cttcgcgcag
    11101 gttgagcttc tcacgcgaag tgacaagcac tttaacctcg ggcgcttggc gcaggatatc
    11161 ggcgacgaga tcgacgccgg ccatcaaatg ctcgaaattg tcgagcaaca acagcaactc
    11221 gcgcgcgcgc aggtgatcga gcagttgcgt tcgaggcgat gcggcgccgg agaagcgcag
    11281 gccgagcgct tcggcgacgg cggtgatcaa gaattcggcg gacatcacgg gcgtgagcga
    11341 gacgtaactg ataccgttga ggaatgcgtt gacggtgcac gcggcgactt cggtcgccag
    11401 acgcgttttg cccacgccgc ccggcccgac gatcgtcagc aagcggcagt gtggatctgc
    11461 cagcagtcgg gcgaggtccg ccaattccgg ctggcgaccg acgaacgggg tcggctgtat
    11521 cggcaggttg ggcgatcggg acgcggcggc tttgatgcgc tcgtagagcg ccgtcgtctc
    11581 cgtcgtcggc tccacgccca gttcttcggc caatacccgg cgacaggtac cgtactggcg
    11641 cagcgccgcg tcgcgctggc ctgtgcgggc cagcatgagc atcagccggc gatgggcttc
    11701 ttcgcgccac ggatcgagcg cgaccagccg tgacgcgaag cgtagcgcct gatcgaactc
    11761 gccgtgatgg gcatgcagtg tgatgagcgc gtcgagcacc cgccaggctg cctgtcgcca
    11821 gcgctcgcgc tcggtgatca gccaggtttc gaactcgggg cagtcggcca gcgccacgcc
    11881 ttccatgaac tcgcccctgt acagctcggc ggcggccgcc agtgacgcgg cgtcgccttt
    11941 cgcgtgcaat tcccggaacg tcacaatatc tacgtcaacg gcgggcgcgg gttgaaagtg
    12001 aatcgtgcgc cgatcggtct cgaggcaacc cggcaaaatc gtagacagat tgtgcagcac
    12061 gcggcgcaat tcgcttcgcg cctgatcgtc gctcgcgtcg ggccagaaca ggccgatcag
    12121 atgatcgcgc gagatcgggc gcgcttcggc ggccaggtag cccagcagcg cgattgcttt
    12181 gggtgagcgg aagcgcgggg ccttaccttt ttcccagatg acctgcaccg gccccaacaa
    12241 cctcaactcg atcggcattg aaggctttgg cgtcatgcgc tcccggctcg ccgtgcggac
    12301 gatttgcgga cggtgccatt atacctttct cgcacattaa cctgaatatg accgatggcc
    12361 catacgccag actccgattc acgcgatatg cgaccgtacc aggcttacct tgttcgctgc
    12421 tggcgcgagg ggtcggactg gcgcttctcg ctggaaggca ttgggcgcga ccgccagcgg
    12481 ttgggcttca gtcgtttgga ggaactgatg gcgcatatcc aggccagtct atcagaggag
    12541 ggtgatgacc aatcaagcga attcccggat ggatcgtgac tcgaacgcgc atttcaatca
    12601 ttttcaaacg gaggctatgt catgactcgc aaaacgctcg tcctcgtttc tgggttggcc
    12661 gcgctgacgc tggtgatcag cttgagcgcg gcagcggcgc aagacgccgg cccgcagacc
    12721 gaatccagcg tgcaggctgc atcagcaatg accggctact acaacttgtt cggctatgag
    12781 gggattggca cgctgggcag cgtaggtttc gccgcggggc caacgccgcc ggtgggcgac
    12841 tacgtcacca cacactgccg ttacatcgac gtgcagaaca cgacggtggg acactactat
    12901 ct
//
LOCUS       DBNHXB010000216         3642 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2230219, whole genome shotgun sequence.
ACCESSION   DBNHXB010000216 DBNHXB010000000
VERSION     DBNHXB010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2230219"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="AC162G_08980"
     CDS             <1..635
                     /locus_tag="AC162G_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5075987.1"
                     /translation="ILEAARALGFGLKIHADEFAPLGGTQLAVELGAASADHLVVTPP
                     REIEALGRSNTVAVSLPGTPFGLGQTAFTPAQAILGAGGILALATDCNPGTTWCESMQ
                     MVIAVACRYLRLTPAQAIVASTLNAAHAIGLGDRIGSLQAGRQADIIILDAPDYRHLG
                     YRYGVDLVEKVIKRGRRVTHAAGALVSSSVETRQGRMDNSVAMTERGGIR"
     gene            632..1108
                     /locus_tag="AC162G_08985"
     CDS             632..1108
                     /locus_tag="AC162G_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5075988.1"
                     /translation="MKVRDIMTTDVVSAKRGTSVNELAKLMGARDISGVPVLDDDGSV
                     VGIVTELDMIARNTRLDMPRFIQILDLGRIPLERRSDYEHRMRHMLGTRAEDLMSEEV
                     VTVGPDDSVEDLAELMVKRRVNPVPVVENGKLVGIVSRADIIDMMAAEVGEPPGEQ"
     gene            complement(1111..1569)
                     /locus_tag="AC162G_08990"
     CDS             complement(1111..1569)
                     /locus_tag="AC162G_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075989.1"
                     /translation="MKKSPFTPVRIVVTIACMLALASLVIAAPALPAQVMSLGSARSR
                     WMQIESPISTPLASPLFPTVVSPLPTPTSEPLECSSALPCTDDAPTPQSTFQAFLPGL
                     SEPDSVPARPPGPPPDLGTILNYVAAAVVVIGVTLKVYWFISDRRKGASK"
     gene            complement(1689..3446)
                     /locus_tag="AC162G_08995"
     CDS             complement(1689..3446)
                     /locus_tag="AC162G_08995"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HIR5075990.1"
                     /translation="MLGYKREELLQMNIADLIPPEDLAAQPIHFAELRAGQTVLTERR
                     LRRKDGSLVLVESSARMLEDGRIQAITRDITERKRVEAALRESEERLRLAVSAAQMDT
                     WEWDILADRTSSGGYRQMAPPAGTYESFMARVYPADREVIEQAVQRTVENGAPYDVEF
                     RTVRPDGSLRWQASQGQTLRDESGRAVRLIGVTQDITERKQAEEALRQAQKMESLGVL
                     AGGIAHDFNNLLVAMLGQASLALAQLPPESAARAHVEKVVKAAERAADLTRQMLAYSG
                     RGQFEKRTVNLNKLIHENLHLFEVAVPKNVQLRSLPAEPLPAIEGDVGQLQQVIMNLI
                     INAAEAIGERPGTVRVTTGVQAVSAADDQLWRHTGEPLAPGEYVVLEVSDDGSGMSVE
                     TLSKIFDPFFTTKFTGRGLGLAAVLGIVRGHRGGLSVDSEVGRGTTFRLFFPAGGEAS
                     SEPIAPRAAAPAGAPRGRVLVIDDEAPVREAVADILEIEGVQVVSAATGAAGIDLYRD
                     RAAEIDLVLLDLSMPGMTGDETLRRLREINPDIQVILSSGYSEAEATRRFTGLGLVGF
                     IQKPYDAASLVEIVREHLA"
BASE COUNT          627 a         1184 c         1196 g          635 t
ORIGIN      
        1 aaatcctgga ggcggcgcgg gcgctcggct tcggcctgaa gatccatgcg gacgaattcg
       61 cgccgctggg cgggacgcag ctcgccgtcg agctgggcgc ggcgagcgcg gatcatctcg
      121 tcgtcactcc accgcgcgaa atcgaggccc tgggccggtc aaacacggtt gccgtgtcgc
      181 tgccaggcac gccgttcgga ttggggcaga cggctttcac tcccgcgcag gcaatcctcg
      241 gcgcgggcgg cattctggca ttggccaccg attgcaaccc gggcacgacc tggtgcgagt
      301 cgatgcagat ggtgatcgcc gtcgcctgcc gctatttgag gctcacaccc gcgcaggcaa
      361 tcgtcgcgtc gacgctgaac gcggcgcacg cgatcgggct aggagatcgg atcgggtcgc
      421 tgcaagccgg ccggcaggcc gacatcatca tcctggatgc cccagactac cggcacctcg
      481 gctatcgcta cggggtcgac ttggttgaga aggtgattaa gcgcggccgc cgggtgacac
      541 acgcggcggg agcgcttgtg tcttcatccg tcgagacgcg ccaagggcgt atggacaact
      601 ccgtggctat gaccgagaga ggaggaataa gatgaaggta cgagacatca tgaccaccga
      661 cgtcgtcagc gcgaagcgcg gcacgtcggt gaacgagctg gccaagctga tgggcgctcg
      721 cgacatcagc ggcgtgccgg tgctcgacga cgacgggtcg gtcgtaggta tcgtcaccga
      781 gctggatatg atcgcgcgca acacgcggct ggacatgccg cgcttcattc agatactcga
      841 cctgggccgc atcccactcg agcggcgcag cgattacgag caccgcatgc ggcacatgct
      901 gggcacgcgc gccgaggatc tgatgtcgga agaggtcgtg acggtcggcc cggacgacag
      961 cgtggaagac ctggccgagc tgatggtgaa gcgccgcgtc aaccccgtgc cggtggtcga
     1021 gaacggaaaa ctggtcggca tcgtcagccg cgcggacatc atcgacatga tggcagcaga
     1081 ggtgggggag ccgccaggag aacagtagcg ttacttcgat gcacccttgc gccgatcgga
     1141 aatgaaccag tagaccttca gcgtcacgcc gatcaccacg acggcggcgg cgacatagtt
     1201 gaggatcgtg cccagatcgg gcggcggccc cggcgggcgg gcgggcacgc tatccggctc
     1261 agagagaccc ggcaagaacg cctggaaagt actctgcggc gtgggcgcat cgtcagtgca
     1321 cggcagagcc gagctgcatt cgagtggctc ggaggtaggc gtcggcagcg gactcacgac
     1381 agttggaaac agcggactag ccaggggggt cgaaatcgga ctctcgattt gcatccatcg
     1441 cgagcgcgct gatcccagtg acataacctg cgcgggcaac gcaggcgcgg cgatcaccag
     1501 gctggcgagg gccagcatgc aggcaatcgt gacgacgatt cgaaccggcg tgaaagggct
     1561 tttcttcacg atgcgacctc gtgagggcct caaggctctc gcaaggccgc agtttaccat
     1621 tcaacggcga aacgcgcacg ccgcgccgcg cccgtatgac cgatccgttc ctgtctcagg
     1681 gaggccggtc aagccagatg ctcccggacg atctcgacga gggatgcagc gtcgtacggc
     1741 ttctgaatga agcctaccag cccgaggccc gtgaagcgcc gggtcgcctc cgcttcgctg
     1801 tatccggagg acagaatcac ctgaatgtcg gggttgatct cacgcagccg gcggagcgtc
     1861 tcgtcgccgg tcatgcccgg catcgaaaga tccagtagca ccaggtcgat ctcggccgcc
     1921 cgatcccgat acaaatcgat gcctgccgcg cccgtagccg cgctgacgac ctgcacgcct
     1981 tcgatctcga gaatgtcggc gacggcctcg cgcaccggcg cttcgtcgtc gatgacgagc
     2041 acacgcccgc gaggtgcgcc ggccggcgca gccgcacgcg gcgcgatcgg ttcactcgac
     2101 gcttctcccc cggccggaaa gaagagccgg aaagtcgtcc cgcgcccgac ctcgctgtcg
     2161 accgacagtc caccccggtg cccgcgcacg atacccagca cggcggccag ccccaggccg
     2221 cgccccgtga acttcgtcgt gaaaaacggg tcgaaaatct tcgacaacgt ctcgacgctc
     2281 atgccgctgc cgtcgtcgct cacctccagc acgacgtact cgccgggggc aagaggctct
     2341 cccgtgtgcc gccagagttg atcgtctgcg gcgctcaccg cctgcacgcc ggtcgtcacc
     2401 ctgaccgtgc cgggtcgctc gccgatggct tcggccgcgt tgataatcag attcatgatg
     2461 acctgctgca actggccgac gtcgccttcg atcgccggca gcggctcggc tggcagcgag
     2521 cgcaactgca cgttcttggg cacggcgacc tcgaacagat gcaggttctc gtgaatcagc
     2581 ttgttcagat tgacggtgcg cttctcgaac tggccccggc cggaataggc gagcatttgt
     2641 cgggtcaggt cggcggcgcg ctcggcggct ttgacgactt tctccacgtg cgcgcgggcc
     2701 gcgctctccg gcgggagttg cgccagcgcg agtgaggcct gccccagcat cgccaccagg
     2761 aggttgttga agtcgtgggc gatgccgccg gcgagcacgc ccaggctctc catcttttgc
     2821 gcctggcgca gcgcctcttc cgcctgcttg cgctcggtga tgtcttgcgt gacgccgatc
     2881 aggcgaacgg cgcggcccga ttcgtcgcgc agtgtctggc cctgtgacgc ctgccagcga
     2941 aggctgccgt cgggtcgaac ggtgcggaat tctacatcgt agggtgctcc attctcgacg
     3001 gtgcgctgca cggcctgctc gatgacttcg cggtctgctg gataaaccct tgccataaaa
     3061 gactcatagg ttccggccgg aggagccatc tggcggtatc cgccggagct tgtccggtcg
     3121 gcgaggatgt cccactccca ggtatccatt tgggcggccg acacggccag ccgcagccgc
     3181 tcctcgctct cgcgcagcgc cgcctccacg cgcttgcgct cggtgatgtc gcgcgtgatg
     3241 gcctggatgc gcccatcttc gagcatgcgc gcgctggatt ctacgagcac cagactcccg
     3301 tccttgcgtc gcagccggcg ctcggtgagc accgtctggc ctgctcgcag ctcggcgaag
     3361 tgaatgggct gggcggccag gtcttccggc gggatgaggt cggctatgtt catctgcagc
     3421 agttcttcgc gtttatagcc cagcagatcg catgctttct agttcacttc gatttaattt
     3481 ccttccgggt agatgatgga tatgacatcg gcagcctgtt ccaccaacat gcggtacttg
     3541 tggacattct cacgcagcgc cgcctccacg cgcttacgct cggtgatgtc gcgctcgatg
     3601 gcctgaatgc gcccatcctc gagcatgcgc gcgctggttt cc
//
LOCUS       DBNHXB010000217         5974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3572673, whole genome shotgun sequence.
ACCESSION   DBNHXB010000217 DBNHXB010000000
VERSION     DBNHXB010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3572673"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..2648
                     /locus_tag="AC162G_09000"
     CDS             255..2648
                     /locus_tag="AC162G_09000"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR5075991.1"
                     /translation="MAGLELTPFESVAIWAVLGVAILGLAYALFLRNQILREDKGTSK
                     MQEVWGAIREGADAYLGRQLRAILPLIALLTGALFLSVYIVPPSPEAIERFAGMDESQ
                     IRLIVGLARAVAFVMGAGFSLTVGQLGMRMAVQANVRVAAAARRSFGDALRIAYRAGT
                     ITGMLTDGLGLFGGTIIFIIMGKAAPDALLGFGFGGTLLALFMRVGGGIFTKAADVGA
                     DLVGKVEAGIPEDDPRNPAVVADLVGDNVGDCAGMAADIFESYEVTIVSALILGLALT
                     SITGRIEWILYPLLVRGIGVLSSIIGTYLVRGGPGKSGDAMRAIFTGFLSSAAISVLL
                     FGLMAGFYLSDSVPGGTTIPLLGIYVGGWWRPFLTTTVGVLLAILIDRLTEYFTSTHN
                     MPVKGIKKAADTGPATLILQGVAVGYESSVWAIIVIAITIFASILIFGSIPEVSATDR
                     ITFILYGVAMTGIGMLTLTGNNVAMDSYGPISDNANGIGEMAWSGMEDEETKAAQQIM
                     ADLDAVGNTTKAITKGVAIGSAVIAAVSLFGSFLVDVSRAQATLGVPAPDRIQAIGIR
                     VDVPQVFVGMLIGGALPWLFSSFAIQAVSRAASLIVQEVRRQFKLGVLEGKVKPDYQQ
                     AVSISTAAAQKELVPLALMGVITPVLVGLVLQVEALGGFLAGIILSGQLLAVFMNNAG
                     GAWDNAKKLIEDEPKDPANNLGKGSERHKAAVVGDTVGDPMKDTAGPALNPMIKVVNL
                     VSVIIAPIVVQYATAAGAAQAVIWVVSLVLLAVLAWAIRTSKREAPAPIAAQPATGK"
     gene            2714..3754
                     /locus_tag="AC162G_09005"
     CDS             2714..3754
                     /locus_tag="AC162G_09005"
                     /EC_number="3.5.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF013046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000118 - histone deacetylase complex
                     [Evidence IEA];
                     GO_function: GO:0004407 - histone deacetylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR5075992.1"
                     /translation="MATAYVYHPTYLEHDHPGHVECRERLEQVNEALDQTGMRSRLQP
                     LKPQPITLERLIRVHLPRYVEHVEHVARSGGGGLAGRGDETYVAPRSYEAALLAAGGV
                     VTAVESVARGEFKNAFALVRPPGHHAFADHGEGFCLFNNVAVAAATARHDLGIERVLI
                     VDFDLHHGNGTQSIFYDDDTVLLFSTQQWGIYPGTGHWSETGVGKGAGFTVNVPMIPG
                     WGDSAYARFFNDLLAPIARRFRPQFMLASAGYDPHWTDLLGSMTVSDRGFFDLTRRLV
                     ALSEELCGGRLVLTLEGGYGLQGLAYGVVATFAALLGDETFADPVGPSPRPERPFDEK
                     YLRQLRELHGLA"
     gene            3849..>5974
                     /locus_tag="AC162G_09010"
     CDS             3849..>5974
                     /locus_tag="AC162G_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075993.1"
                     /translation="MADIALQDYVTEIESLIEGNAYDEAVAHCRHILGVYPKYLEAYR
                     LLGKAALEKEDDRAALDIFERVLSVDPEDFVARIGLSIVRDRQDTLDEALWQMERGFE
                     LMPSNDVIQSELRRLYGRRDGVEPQYISLTRGALARMYAHGDLYDEAIAELRALLRES
                     PERLDLQVLLAETLWRNDQRSEAASVAEGILEKLPYCLTAGLILGEIYLNSGLADEGE
                     MLLRRAQAIDPDNLRANALLGPASPLAEQKTTVERLERGLQFTPVSPQAAVEEVPEWL
                     SGLAGLTLEEPESLSVPGLPPPPSGPPIELGPPTEGEIPEWLESMELPAESSAETPEW
                     IDTLWQQAQAPAEAPVESAGAGAEPTPAGAQPSESLPDWLQAGLAETPGAAEETAPPD
                     WLSEIAAPPVAEMAEQPELPSWLSDLESETAGMEQAGQEALDLTQADLPDWLKALRPQ
                     VGEAPPPAVELPEVQESEIPDWMADLEEKPSADEAFDFLRRLGESMEAEPEAPPATEQ
                     PVQPAAPAEMGAMPSADEALAFLQGLTAGQEERLRAEAEQAAEERMEAIVGGTPGTSP
                     LRPPPQLAPEAPSVSEQPAEMAAMPSADEALAFLQGLTVGKEEQLRAEAERAADERMD
                     AIMGGKPGTSPLRPRAAPAPEVVEVTPEPEVAPLAEDLLATDEALAFLQDLAVPPEAP
                     SAGELEYAEMVEAPGVALDFWLQTAE"
BASE COUNT         1063 a         1789 c         1983 g         1139 t
ORIGIN      
        1 tgtattcatt cgcgcacgag tcgaatgacc tgagcatcct taaagtgtat cgtggatcgc
       61 gacaaccgcc aagtggatcc catgagagtc gagacgagct acagatccac ttgacttatc
      121 aatagaatag gctagaatgt gcaaaatcat tcacagacgc aaacacgttc gcaaaccgat
      181 ttgagatgcc cgtccctgcg ggcgtttttg tgcgtgtttg cgccggatct attttcgaaa
      241 cggggagtgg atccatggcc ggactcgaac tgacaccctt tgagagcgtt gcgatctggg
      301 ccgtattggg agtggccatc ctcggactgg cctacgcgct tttcctgcgc aaccagattc
      361 tgcgcgaaga taaaggcacc tcgaaaatgc aggaagtgtg gggcgcgatt cgcgaaggcg
      421 cagatgctta tctcggccga cagttgcggg cgattctccc gcttatcgcg ctgctgacag
      481 gcgccttatt cttgagcgtg tatatcgttc cgccgtcgcc tgaagcgatt gagcgcttcg
      541 ccggaatgga tgagagtcag attcgattga ttgtcggcct ggctcgcgcc gtggcgttcg
      601 tgatgggcgc cggcttcagt ttgacggtcg gccagttggg catgcgcatg gccgtgcaag
      661 ccaacgtccg cgtggccgcc gccgcgcgcc gatccttcgg cgacgcgctg cgcatcgcct
      721 accgcgcggg cacgatcacg ggcatgttga ccgacggcct gggcctgttc ggcggcacga
      781 ttattttcat catcatgggg aaagccgctc ccgatgcgct gctcggcttc gggttcggcg
      841 gcacactgct ggcgctcttc atgcgcgtgg gcggcggtat ctttacgaag gctgccgacg
      901 tcggtgccga tctggtgggc aaagtcgaag ccggcattcc ggaggatgac ccgcgtaacc
      961 cggccgtcgt cgccgatctg gtgggcgaca acgtcggcga ctgcgcgggc atggccgccg
     1021 atattttcga gtcctatgaa gtcacgatcg tctcggcgtt gatcctcggc ctggcgctga
     1081 cttcgattac gggacgcatc gagtggattc tctacccgct gctggtgcgc ggcattggcg
     1141 tgctgtcgtc gatcatcggc acgtacctgg tgcgcggggg gccgggcaaa tccggcgatg
     1201 cgatgcgggc catcttcacc ggcttcctgt cgtcggcggc gatctcggtg ttgctgtttg
     1261 gtctcatggc aggcttctac ctcagtgaca gtgtccctgg cggaaccacg attccactgc
     1321 ttgggatcta tgtcggtggc tggtggcggc ccttcctgac gacgacggtt ggcgtgctgc
     1381 tcgccatcct gatcgaccgg ctgaccgaat acttcacgag cacccataac atgccggtga
     1441 agggcatcaa gaaggccgcc gataccggtc ccgccacgct aatcctgcag ggcgtggcgg
     1501 tcggctacga gtcatcggtg tgggcgatca tcgtcatcgc gatcaccatc ttcgcgtcta
     1561 tccttatatt tggcagcatc ccggaggtat cggccacgga ccggatcacc tttatcctgt
     1621 atggcgtagc tatgacaggc attggcatgc tgaccctgac cggcaacaac gtggcgatgg
     1681 attcctacgg gccgatctca gacaatgcta acggcatcgg tgagatggcc tggtcgggca
     1741 tggaggatga ggagacgaag gcggctcagc aaatcatggc cgatttggac gcggttggca
     1801 atacgacgaa ggcgatcaca aagggcgtag ccatcggctc ggccgtcatc gccgccgtgt
     1861 cgctgtttgg ctcgttcctg gtggatgtga gtcgcgcaca ggcaacgctc ggtgtgcccg
     1921 cacctgaccg gatccaggct attggcattc gtgtggacgt tccccaggtg ttcgtcggga
     1981 tgctgatcgg cggcgcgctg ccgtggctgt tttcgtcgtt cgcgatccag gcggtttcgc
     2041 gcgcggcctc gctcatcgtc caggaagtgc ggcgccagtt taaattgggt gtgttggagg
     2101 ggaaggtcaa gccggattac cagcaggctg tatcgatctc gaccgcggct gcgcagaaag
     2161 aactggtgcc gcttgcattg atgggtgtga ttaccccggt cctcgtcggc ttggtgctgc
     2221 aggtggaagc gctgggcggt ttcctggcgg gtatcattct atccggacaa ctgctcgcgg
     2281 tgttcatgaa caatgccggg ggcgcgtggg acaacgcgaa gaagcttatc gaagatgagc
     2341 cgaaagaccc ggccaacaat ctgggcaagg gttcggagcg gcacaaggcc gccgtcgtcg
     2401 gtgacacggt gggcgatccg atgaaagaca ccgctggccc ggcgctcaac ccgatgatca
     2461 aagtggtgaa cctggtgtcc gtaatcatcg ccccgattgt cgtccagtac gctacggccg
     2521 ccggcgccgc ccaggcggtg atatgggtcg tgtcgctggt tctgctcgcc gtgctggcct
     2581 gggcaattcg tacctcgaag cgcgaagcgc cggcgccgat tgcagcgcag ccagcgaccg
     2641 gcaagtagcg ttcggcggca cgtggggcga ctcgaagggt tgcccctgtt cttttatcga
     2701 gtacaactag cccgtggcaa cggcatacgt ctatcatcca acttatctcg agcacgatca
     2761 tcccggtcac gtggaatgcc gcgagcggct ggagcaagtg aatgaagcgc tcgaccagac
     2821 cggcatgcgc tcgcggctgc aaccgcttaa gccgcagccg atcacgctcg agcgcctgat
     2881 ccgtgttcac ctgcctcgct atgtggagca cgtcgagcac gtggcgcgga gcggcggggg
     2941 cgggctggcg ggccgtggcg atgagaccta cgtcgcgccc aggtcatacg aagccgcgct
     3001 gcttgcagcg ggtggtgtgg tgactgcagt cgagtctgtc gcgcgtggtg aattcaagaa
     3061 tgccttcgcg ctggtgcgtc ccccgggcca ccacgctttc gccgatcatg gcgagggatt
     3121 ttgtctgttc aacaacgtcg ccgtcgccgc ggcaacagcc cgtcacgatc tcggcatcga
     3181 gcgggtgctg atcgtcgatt ttgacttgca tcacggcaac ggcacacagt cgattttcta
     3241 cgacgacgac actgtgctgc tcttctcgac ccagcagtgg gggatttatc cgggaaccgg
     3301 gcattggagc gagacggggg tcgggaaggg cgccgggttc acggtaaacg tgccgatgat
     3361 tcccggctgg ggggattcgg cttacgcgcg attcttcaac gacctgcttg ccccgatcgc
     3421 tcgccgcttc aggccgcagt tcatgctggc gtcagccgga tacgatcccc actggacgga
     3481 cctgctcggc tcgatgacgg tcagcgatcg ggggttcttc gatctgaccc ggcggctcgt
     3541 cgccttgagt gaggagctgt gcggcgggcg gttggtgctg acgctggagg gcggctacgg
     3601 gctgcaaggg ctggcctacg gtgtggtcgc cacttttgcg gctcttctcg gcgacgaaac
     3661 cttcgccgac ccggtcggcc cgtctccgcg cccagaacgc ccgtttgacg agaagtatct
     3721 gagacagctc cgcgagttgc acggcctggc ctgaaataac ccgacttgca acacaatttg
     3781 agttatgcta agcttaatgg tgaggttgta aggcgccgca ttgaagaact accgagaagg
     3841 attttgcgat ggccgacata gcacttcagg attatgtcac tgagatagag tcgcttattg
     3901 agggcaatgc ctacgacgaa gccgtcgccc attgccgcca catcctgggc gtctatccga
     3961 agtacctgga agcctaccgt ctgcttggca aggccgccct ggaaaaagaa gacgaccgcg
     4021 cggcgctcga catcttcgag cgcgtcttga gcgtcgatcc ggaggacttt gtcgcgcgca
     4081 ttggcctcag catcgtgcgc gatcggcagg acacgctcga cgaggcgctg tggcaaatgg
     4141 agcgcggctt cgagctgatg ccaagcaacg acgtcattca gagcgagctg cggcggctct
     4201 atggccggcg cgacggcgtt gagcctcagt acatctcgct cacgcgcggc gcgctggcgc
     4261 gcatgtacgc tcacggcgat ctgtacgacg aggctatcgc ggagctgcgc gcgctgcttc
     4321 gagagtcgcc cgagcgtctc gacctccagg ttctcctggc tgagacactg tggcgcaacg
     4381 accagcgaag cgaagccgcc agcgtagccg aaggcattct cgaaaagctg ccctactgcc
     4441 tgacggccgg cctgatcctt ggcgagatct acctcaacag cggtcttgcg gacgaaggcg
     4501 agatgctcct gcgccgggcg caggccatcg atccagacaa tctgcgggcg aatgcactgc
     4561 tcggccccgc ctctcctctc gccgaacaga agacgacggt ggaacggctg gaacgggggc
     4621 tgcagttcac gcctgtctct ccccaggcag ccgtggaaga agtgcccgaa tggctttcag
     4681 gcttagccgg actgacactc gaggagcctg agtccctctc cgtgcctggc cttcccccac
     4741 cgccgtcagg cccgccgatt gagcttggtc cacccaccga aggcgagatt cctgaatggc
     4801 tggaaagcat ggagcttccg gcggagtcct ccgcagagac gccggagtgg attgatacac
     4861 tctggcagca ggcgcaagcg ccggccgagg ctccggtgga atcagcaggc gccggcgctg
     4921 aacctacacc ggccggcgcg cagccgagcg agtctttgcc cgactggctg caggccgggc
     4981 tggctgaaac gcccggggcc gcggaggaaa ctgcgcctcc cgactggttg tccgagatcg
     5041 cagcgccgcc cgtggctgag atggccgagc agcctgaatt gccaagttgg ctgagcgacc
     5101 tggagagcga aacggctggt atggagcagg ccgggcagga agcacttgat ctcacccagg
     5161 ctgacctgcc tgattggttg aaagcactgc gtccacaggt cggcgaggcg ccgccgcctg
     5221 ccgtcgaatt gcccgaggtg caggagtccg agattccgga ctggatggca gacctggaag
     5281 agaaaccctc cgccgacgag gcattcgact tcttgcgaag gcttggcgag agcatggaag
     5341 ccgagcccga agcaccgcct gccacggaac aaccggtgca gcctgccgcc ccggctgaga
     5401 tgggggccat gcccagcgcg gacgaggcac tggcctttct acagggcttg acggcagggc
     5461 aggaggagcg gttgcgcgcc gaggccgagc aggctgccga ggagcgtatg gaggccatcg
     5521 tgggaggcac gccgggaacg tcaccgctgc gacctcctcc ccaattggcg cccgaggcgc
     5581 cgtccgtctc ggaacagccg gcggagatgg cggctatgcc cagcgcagac gaagcattgg
     5641 ctttcctgca gggcctgacg gtgggcaagg aagagcagtt gcgtgccgaa gccgaacggg
     5701 cggccgacga gcgcatggac gccatcatgg gaggcaagcc gggaacgtcg ccgctgcggc
     5761 ctcgcgccgc gccggcgcct gaggtggtcg aggtcacgcc tgaacctgaa gtcgccccac
     5821 tcgcggaaga cctgctcgct acggacgaag cgctggcgtt tcttcaagac ctggccgtgc
     5881 cgcccgaagc gccttcggcc ggcgagctgg aatacgcgga aatggtcgaa gcgcctggcg
     5941 tggcgctcga tttctggctg cagaccgccg agga
//
LOCUS       DBNHXB010000218         4430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1392700, whole genome shotgun sequence.
ACCESSION   DBNHXB010000218 DBNHXB010000000
VERSION     DBNHXB010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1392700"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..2091
                     /locus_tag="AC162G_09015"
     CDS             193..2091
                     /locus_tag="AC162G_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5075994.1"
                     /translation="MAANAWALPTRWSPRQRRRAAFVALIVLEVLLLIFLGTRLVQVQ
                     AVEDRREIVGNVETIHFRNEVVGDPPLLALIVLGALLPLASGRLRREPVMLIVTQAAI
                     VSLIVFVAWPLAQVFGEGFRAGQSEEGFSLVQFEKLLETPMVARASLNTMVIGVTSAA
                     LATVIGTLVAYTLTLTDVPWRRGLRILTILPLVSPPFAVSFAVILLFGRRGLITYDLL
                     HITDYNIYGPQGIVFVQLISDIPLVALILSAVFASISRDLEEAAEDLGGRPLYVLRTV
                     TFPLVTPAILTAALLSFISAISDFGNPMLVGGGYQVLATQAFIQMIELFDLQLGAALG
                     MLLLIPAVLAFLLQHWITNRRSYITVTSGARTGTVRRLPGWIKWPLFGLCVFLAAFNI
                     LLYGSIFVGAFVNQWGFDFTLTLRNLSGLGNAMPMLRNSLIVAVGAGIVGGIAGIVLA
                     WLVTRPHVPGRRALDFSATVMYAVPGTVVGIGYIIAFNAAPYFWTGTFFIIIVAYAFR
                     RLPVGLRTGVATQKQIDPSLEEASLDLGASRFRTFTRITFPLLSRAFFAGVIYIFIRA
                     MTDLSAAVFLNAGNTQLYTVRMFRVMITGTPSQAAAFAALLIVIILIALGLLAKVTGK
                     SFVDLFRV"
     gene            2094..3167
                     /locus_tag="AC162G_09020"
     CDS             2094..3167
                     /locus_tag="AC162G_09020"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005871054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5075995.1"
                     /translation="MSEVFATASIQLKNLRKQFGPVRAVDDVSLEVEPGTLMCLLGPS
                     GCGKTTTLRMIGGFEEPTSGRIFIGADDVTGVPPYARPTAMVFQSYALFPHMSVYDNI
                     AYGLRARRMPRAEIEMRVQEAIALMELHGHAKKSPPQLSGGQQQRVALARALVIHPKV
                     LLFDEPLSNLDAQLRVRMRAEIRAVQRRLGITSVYVTHDQEEAFGISDRVALMNHGKL
                     IQVGTPRELYRQPADRFVAEFVGLSNIVSAELVESSANGAVVRVFGRTIRSRRPPRDP
                     GKPISLVLRPEALKVEPQADSGVPVRVLTLAFLGPLARYTVAVSDGTVLTVDLHNPGP
                     DQFFAEGTPVSLCLPAEVPALLN"
     gene            3413..3877
                     /locus_tag="AC162G_09025"
     CDS             3413..3877
                     /locus_tag="AC162G_09025"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5075996.1"
                     /translation="MIPGAGEMARELKRRGYPLALIADGRPGTYVNVLAQHGLYDLFD
                     AFAISERLGVEKPHPLMFRHALDQLRIRQEDYGRTLMVGNNLERDVKGANELGMISVW
                     IDWAPRRSKTPADASQVPQHTIKMPFDLLGVIDQLEGDRGHAWPFARKFGSA"
     gene            3844..>4430
                     /locus_tag="AC162G_09030"
     CDS             3844..>4430
                     /locus_tag="AC162G_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR5075997.1"
                     /translation="MAIREKIRVGLIGAGRIGQLHAEHLATRIPGVELAAVADVNLAA
                     AQATAARFDVPAAVADYRALLDDRSIRAVAICSATDTHASITQDAASAGKHIFCEKPI
                     DFDLRRIDQTLEAVERAGVKFQVGFNRRFDANFRRVRDLVAEGKVGVPHILRITSRDP
                     EPPPIDYVEVSGGIFLDMTIHDFDMARYLMDDEVR"
BASE COUNT          725 a         1444 c         1420 g          841 t
ORIGIN      
        1 agatggcagg agctgatcgg gtcgaagcga ggcggcggct agcacatcgg ccgttcgccg
       61 ctcgcggctc gccttgagtc cggactcgaa tgcgccccac tctaactcca tactctaact
      121 ccacgaagag cacgcaggag aatacctcgt gaacttcgtg ctcttcgtgg acgactggca
      181 gtgaggtcat cgatggccgc caatgcatgg gcgctcccga cacgctggtc cccgcggcag
      241 cgacgacgcg cggccttcgt cgcgctgatc gtgctcgaag tgttattgct gatcttcctt
      301 ggcacccggc tggtgcaggt tcaggccgtg gaagatcggc gcgaaatcgt cggcaacgtg
      361 gagacgattc actttcgcaa cgaggtcgtc ggcgatccgc cgctgctggc gctgatcgtg
      421 ctgggcgcgc tgctgcctct ggcatcgggc cggctgcgcc gcgagccggt gatgctgatc
      481 gtgacgcagg cggcgatcgt ctcgttgatc gtgttcgtgg cctggccgct ggcacaggtg
      541 ttcggcgaag gcttccgggc cggccagagt gaagagggct tctcgctggt gcagttcgag
      601 aagttgctag agacgccgat ggtcgcccgc gcctcgctga acacgatggt gatcggcgtg
      661 acgtcggctg ccctggcgac ggtgatcggc acgctggtcg cctacacgct gacgctgacc
      721 gacgtgccgt ggcggcgcgg gctgcgcatc ctgacgatac tgccgctcgt ctcgccgccg
      781 ttcgccgtga gttttgccgt catcctgctc tttggccggc gtgggctgat cacctacgac
      841 ctgttgcaca tcaccgacta caacatctac ggcccgcagg gcatcgtctt cgtccagctc
      901 atcagcgaca tcccgctggt cgcgctgatt ctgtcggcgg tgttcgcctc gatcagtcgc
      961 gacctggaag aggcggccga agatctagga gggcggccac tctatgtgct gcgcacggtc
     1021 actttccccc tggtcacgcc ggccatcctg accgccgcgc tgctcagctt catctcggcg
     1081 atctccgact tcggcaaccc gatgctggtc ggcggcggtt atcaggtatt ggcgacgcaa
     1141 gcgttcatcc agatgatcga actgttcgat ctgcaactcg gcgcggcact gggcatgctg
     1201 ctgctcatcc cggcggtgct cgccttcctg ctgcagcact ggatcaccaa ccgccgctcg
     1261 tacatcactg tcactagcgg cgcgcgcacc ggcaccgtgc gcaggctgcc gggctggatc
     1321 aagtggccac tcttcggcct gtgcgtcttc ctggcggcat tcaatattct gttgtatggc
     1381 tcgatcttcg tcggcgcgtt cgtcaaccag tggggcttcg acttcacgct gacgctgcgt
     1441 aatctcagcg gtctgggcaa cgcgatgccc atgctgcgca actcactgat cgtcgcggtc
     1501 ggcgccggga tcgtcggcgg gatcgccggc atcgtgttgg cctggctggt gacgcggccg
     1561 cacgtgccgg gccggcgcgc gctcgatttc tcggcgacgg tgatgtacgc cgtccccggc
     1621 acggtcgtcg gcatcggcta catcatcgcc ttcaacgccg caccgtattt ctggacgggc
     1681 accttcttca tcatcatcgt cgcttatgcc ttccggcgac tgccggtcgg gctgcgcacc
     1741 ggcgtcgcca cgcagaagca gatcgacccg tcgctggaag aagcgtcgct cgacctgggc
     1801 gcgtcgcgct ttcgcacgtt cacgcgtatc acctttccgc tgctcagccg cgcgttcttc
     1861 gccggcgtga tctacatctt catccgcgcg atgaccgacc tgagcgcggc ggtgtttctc
     1921 aatgcaggca acacgcagct gtatacggtg cgcatgttcc gtgtcatgat caccggcacg
     1981 cccagccagg cggctgcgtt tgccgcgctg ctcatcgtca tcatcctgat cgcgctcggc
     2041 ctgctcgcca aagtgaccgg caagagtttc gtcgatctgt ttagggtgta gctatgtctg
     2101 aagtcttcgc aacggcatcc attcaactga agaacctgcg caagcagttt gggccggtca
     2161 gggccgtgga cgacgtcagc ctggaggtcg agccgggcac gctgatgtgc ctgctcggcc
     2221 ccagcggctg cgggaagacg acgacgctgc gcatgatcgg cggcttcgag gagccgacgt
     2281 cgggccgcat cttcatcggc gcggacgacg tgaccggcgt gccgccctac gcccggccga
     2341 cggcgatggt cttccagagc tacgcgctct tcccgcacat gagcgtgtac gacaacatcg
     2401 cctacggtct gcgcgcgcga cgcatgcccc gggccgagat cgaaatgcgg gtgcaggagg
     2461 ccatcgcctt gatggagctg cacgggcacg ccaagaagtc gccgccgcag ttgtcgggcg
     2521 ggcagcaaca acgcgtggca ctcgcccgcg cactcgtcat ccatcccaag gtgctgttgt
     2581 ttgacgagcc gctgtcgaac ctcgacgcgc aactgcgcgt ccgcatgcgg gcagagatcc
     2641 gcgccgtgca gcggcgcctg ggtatcacca gcgtgtatgt cacgcatgac caggaagagg
     2701 cgtttggcat ctccgaccgc gtcgcgctca tgaaccacgg gaagctgatc caggtcggca
     2761 cgccgcgcga actgtatcgc cagccggccg atcgcttcgt cgccgagttc gttggcctgt
     2821 cgaatatcgt ttcggcggag ctggtcgagt cgagtgcgaa cggcgccgtc gtgcgcgtgt
     2881 tcgggcgcac gatccgcagc cggcgccccc cgcgcgaccc cggcaagccg atctcactcg
     2941 tgttgcgccc tgaggcgctc aaagttgaac cacaggccga cagcggcgtg cctgtgcgcg
     3001 tgctcacgct cgcgttcctc ggcccactcg cacgctacac cgtcgccgtg agtgacggta
     3061 cggtgctcac cgtcgatctc cacaaccccg gcccggatca attcttcgcc gaaggcacgc
     3121 cggtcagcct gtgtctgccg gctgaagttc cggcgctgct gaactgatat gcggggaacg
     3181 gtatggggca attagtaatc agtagtcagt catcagtaat cagtaatcgt gccctggctg
     3241 attgcggaag gtgcatcact gatgactgat cactgacgac tgatcactga tcagttaaag
     3301 gaggtgtgcg ctgaccagac acatcctcgc cgtctgcttc gactttggtg acacgctggt
     3361 cgacgagggc acggaaatca aagacgacac gctcaccacg ctgcatgccg agctgatccc
     3421 cggcgcgggt gagatggccc gcgagctgaa acgacgcggc tacccgctcg cgctcatcgc
     3481 cgacggccgc ccgggcacct acgtcaacgt cctggcgcag catggcttgt atgacctgtt
     3541 cgatgcgttt gccatctccg agcgcctcgg cgtcgagaaa ccgcatccgc tgatgttcag
     3601 gcacgcgctc gaccagttgc ggattcgtca agaggactac ggtcgcacgt tgatggtggg
     3661 caacaacctg gagcgtgacg tgaaaggcgc gaacgagctg gggatgatca gcgtgtggat
     3721 cgactgggcg ccgcgccggt cgaagacgcc cgccgacgca tctcaggtgc cgcagcacac
     3781 gatcaagatg ccgttcgacc tgctcggtgt gatcgaccaa cttgaagggg atagggggca
     3841 cgcatggcca ttcgcgagaa aattcgggtc ggcttgatcg gggctggacg gatcggccag
     3901 ttgcacgccg agcatcttgc aacgcgcatc ccaggcgtcg agctggccgc cgtcgccgac
     3961 gtcaacctgg cagcggcaca ggcgacggcc gcgcgctttg acgtgccggc cgccgtcgcc
     4021 gactatcgcg cgttgctgga cgatcgatcg atccgggcag tggcgatctg ttcggcgacg
     4081 gacacacacg cgtcgatcac gcaggacgcc gcctcagccg gaaagcacat cttctgcgag
     4141 aagccgatcg atttcgacct gcggcggatc gaccagacgc tggaggcggt cgagcgagcc
     4201 ggggtgaagt tccaggtcgg gttcaatcgc cgcttcgacg ccaacttccg ccgcgtgcgc
     4261 gacctggtcg ccgagggcaa agtcggcgtg ccgcacatcc tgcgcatcac cagccgcgac
     4321 ccggagccgc cgccgatcga ttacgtcgaa gtctccggcg gcatcttcct cgacatgacg
     4381 atccacgact tcgacatggc gcgttacctg atggacgatg aggtgcgcga
//
LOCUS       DBNHXB010000219         8553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_386131, whole genome shotgun sequence.
ACCESSION   DBNHXB010000219 DBNHXB010000000
VERSION     DBNHXB010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8553
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_386131"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="AC162G_09035"
     CDS             <1..264
                     /locus_tag="AC162G_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5075998.1"
                     /translation="GDLDTVYAPIAAGQGREEFVGGQQGQEGQTSTNEGKSPQPGANN
                     PALVPYSQVYQQYLQIASQAMERAYIPASLQDYVKEYFSGLEP"
     gene            282..1268
                     /locus_tag="AC162G_09040"
     CDS             282..1268
                     /locus_tag="AC162G_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5075999.1"
                     /translation="MADPITVEQFTAIAQAIEREVGKMIVGQHDIVRQTLVTLLAGGN
                     ALLEGVPGLGKTMLLRTLAQVWDCRFSRIQFTPDLMPADIVGTNILIVDEGGRRRFQF
                     EPGPVFANLVLADEINRATPKTQSAMLEAMQEHSVTAAKITHRLEEPFFVLATQNPLE
                     MEGTYPLPEAQLDRFFFKLNVAYPNVNELVAIADRTTGQELPQPQVVADGATVMAMQQ
                     LARGVPIAPHVTEAAARLVRATQPQDPLAPDIVKRYVNWGSSPRGMQAMILAGKIIAL
                     LDHRYNVAVDDLRQAALPALRHRLILNFEAQAEGVTTDQIVTELVEQVLEYA"
     gene            1261..2166
                     /locus_tag="AC162G_09045"
     CDS             1261..2166
                     /locus_tag="AC162G_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR5076000.1"
                     /translation="MRELLSPSFLATLDRLTLVSRRARAGKFKGERRSPRRGGSVEFA
                     DFREYAPGDDFRQIDWNAYARLERLFLRLFVEEEDATVHVIVDASRSMDWGQPLKSTY
                     ARRVAAALGYVALVGMDRLVGAALGLDGRSYALFPPHRGKHQALAWFDWLAGLALSEA
                     EGLQPGGQASPLAALTQYAASVRRAGPLVVVSDLMDDGWREGIQRLAARHYEITLLHI
                     LAPQEIDPEWEGDLKLVDSETNAAVEITADFDLLSRYRARLEAWQSEWQQFCAARAIN
                     YAPIETSLPFEELVLSFLRRRGILK"
     gene            2177..4189
                     /locus_tag="AC162G_09050"
     CDS             2177..4189
                     /locus_tag="AC162G_09050"
                     /inference="COORDINATES: protein motif:HMM:NF024909.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR5076001.1"
                     /translation="MNFITPLAFGLAALLPVIVALYFLKLRRQEQSVSSTYLWRTLVR
                     DTAANAPWQRLKPNWLLLLQLLFLIALIVALARPFTWSEAAASSHLILVMDTSASMGA
                     TDVEPNRLAEAVTTARELIEGLPNSTRVTVIEAGAQVRVPVSGASDPGAAVAALSALR
                     PGWAGADLTSALTMAAAIAAREPDSEVVILSDGRVTLPDASTPSTSLRAGFTLPGRVR
                     YIPIGRENANQAIGAFSLQAESGRRSLTAFVQMVNFGREDMQRRLVLREAAGGPAPSQ
                     GSGQALSGAEGRLLTVRDVTLPAGKSQALILPGLPGDAHAVEARLEGQDVLSADDRAW
                     AVPPSTEKVAVRIVGPGNRFLETALRLMPNVEITLVQPDQLSDQLSNQPTNQLTIFDS
                     VVPTGTLPGGNLLFIAPPRSTEVFSVTGRIDAPLAAPVVADDPLLRYVDLHEVAVQDA
                     ARLALPTWGRAVVLDTKTDAPLLVAGERDGRRLAVLAFDLRRSDLPLRVAFPILVANL
                     VDALVPGGAAGVPASVEPGQPLVISASPQASAIVVRTPDGQSHTLIPAGGSVVFQQTD
                     RPGAYEIAAQDSAGQLQNLGLFTVNLFNPQESDVSPRETLPVASAGSVSAQELPRARD
                     EWWRPIAWMALVLLVAEWLLVHRGNLARLWSLVTARGRPAGVKRNA"
     gene            4246..7167
                     /locus_tag="AC162G_09055"
     CDS             4246..7167
                     /locus_tag="AC162G_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR5076002.1"
                     /translation="MTLSFLYPTALYALLLLPLLLTLPFVGRRASARTFNFWVGLALR
                     AVVLLGLILSLAGTQVVQAVNDTTVVFVVDRSDSVPRAEQKRAEDFVRASLATMKAGD
                     RAAVVVFGEEALVERLASDDTTLAPITSAPRTTHTHLASALRLALALFPEETHKRIVL
                     LSDGLENVGHAADLVDLAVARGVQIAVMPLAPASGRAEAYLDALEAPAAVRKGQTFEI
                     VAVIYSTSAGPASLRLFGDGKLIATRDLTLQPGDNRVSFSLTGDEPGFHRYTAELQAA
                     GDTLPQNNVASAFSVVHGPPRVLVVENTSGDADNLIAALESAKVETERVAPAVLTGDL
                     ATLSNYDAVFLVNIPARALPDAAMNALPTYVRDLGRGLVMIGGPEAYGAGGYLRTPLE
                     KALPVDMDVRSRTQEPNLALVFAIDKSGSMGRCHCDDPSAQPGSYARIESGLSKVDIA
                     KDAVMQAARAVGRADYVGVVAFDANALWALRVQEMIDPDFVQQKIGGLRAEGQTNINA
                     GLSEAEAALEQTPARLKHVVLLTDGWSRSFEYGALALRLHDKGITLSVIAAGSGSATY
                     LEQLARQAGGRYYAARTMTEVPSLFFKETVEAAGSYIVEEPFYPAPAGATPILRGLDV
                     TRLPALRGYNGTTPKSSAQVSLVSSQGDPLLATWQYGLGRAAAWTSDVKGQWATDWIK
                     WNGFNTFIAQLTSWVLPQPADEGLQTMFASDGEQTVLEVTSKDADGRPRDLLDTHATV
                     VGPDSPAGSGQALVSQAITLTQVAAGRYRGRLPAVQPGTYLVRVTQQDAGGTPVASAT
                     TGLIVPYSPEYKSLGEAASVLPELSRATGGQVLAEPELAFAPLARPASRAQPIWPTLL
                     LAAALLFPLDVAARRLRLTRGDAQRVWAWMRTPLRRRGAAVAPRPRALSTLFAARDRA
                     IGSRSQRMAQPASPVVEGERPSRPPPESREPSPELPASPEAMAARLRRAKDRARKSRP
                     G"
     gene            7474..8118
                     /locus_tag="AC162G_09060"
     CDS             7474..8118
                     /locus_tag="AC162G_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HIR5076003.1"
                     /translation="MHKYILFDLDETLYPRDAGLMQEIGVRILRYMVERMGFTPEAAE
                     AKRPYYYHKYGTSLRGLMAEETVDAEDYLRFVHEVDITRYSGPNPELDAMLRRIPLSK
                     VVFTNATREHARRVLDALGIADHFLFVVDVRAMDYASKPDPQAYRRILDAIGARAEEC
                     IIVEDTPRNLLPAKALGMKTILVDHQACDEVDYCVSDILKVGDVVARLLADDNQ"
     gene            8157..>8553
                     /locus_tag="AC162G_09065"
     CDS             8157..>8553
                     /locus_tag="AC162G_09065"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HIR5076004.1"
                     /translation="MTDKLISLTDAAALVQSGDTLALGGMTLYRRPVAFVRELLKAET
                     HDLTLLCFTASYESDLLVGAGRVKAVRTCYFGMEAFGLAPMFTQRATSGDLRVIEESE
                     GSLAFGMRATLARVGFMPGPGWVGTDMLKV"
BASE COUNT         1472 a         2749 c         2776 g         1556 t
ORIGIN      
        1 ggcgacctcg acacggtgta cgcgcccatc gccgccggtc aggggcgcga ggagttcgtc
       61 ggcggacagc agggtcagga ggggcagacc tcgacgaacg agggcaagtc gccccagccc
      121 ggtgcgaaca accctgcact cgtcccatat agccaggtgt atcaacagta tctgcaaatc
      181 gccagccagg cgatggagcg cgcgtacatc ccggcaagct tgcaagacta cgtgaaagag
      241 tatttcagcg ggctggaacc atgacaggag gcccagtctc aatggccgat cccatcaccg
      301 tcgaacagtt caccgccata gcccaggcca tcgagcgaga ggtgggcaag atgatcgtcg
      361 gccagcacga catcgtgcgg cagacgctcg tgacgctgct ggccggcggt aacgccttgt
      421 tggaaggcgt gcccggcctg ggaaaaacca tgctgttgcg tacgctggcg caggtgtggg
      481 actgtaggtt cagccgcatc cagttcacgc ctgacctcat gcctgccgac atcgtgggca
      541 cgaatatctt gatcgtggac gaaggcgggc ggcggcgttt tcaattcgag ccgggaccgg
      601 ttttcgccaa tctggtgctg gccgacgaga tcaaccgcgc cacgcccaag acgcaaagcg
      661 cgatgctgga agcgatgcag gaacacagcg tcactgccgc caagatcacg catcgcctgg
      721 aagagccttt cttcgtgctg gctacgcaga acccgctgga gatggaaggc acctaccctc
      781 tgcccgaagc gcagctcgac cgcttcttct tcaaactcaa cgtggcctat cccaatgtga
      841 acgaactggt ggcgattgcc gatcgcacaa ccggccagga gcttccgcaa ccgcaagtcg
      901 tcgccgacgg ggcgacggtg atggccatgc agcaactcgc acgcggcgtg ccgatcgcgc
      961 cgcacgtgac cgaagcggcg gcgcggctgg tgcgcgccac gcagccgcaa gacccgctcg
     1021 cgcccgatat cgtcaagcgc tacgtcaact ggggctccag cccgcgcggt atgcaggcga
     1081 tgatcctggc cggcaaaatc atcgcgctgc tagatcaccg ctacaacgtg gcggtagacg
     1141 acctgcgcca ggccgcattg cccgcactgc gccaccggct catcctcaac ttcgaggcgc
     1201 aagcagaagg ggtgacgaca gatcagattg tgacggagct tgtcgagcag gtgttggagt
     1261 atgcgtgaac tgctctcccc atccttcctc gccacgctcg accgtctcac gctggtgagt
     1321 cgccgggcgc gcgcgggcaa gttcaagggc gaacgacgca gtccgcgacg cggcggcagc
     1381 gtcgagttcg ctgatttccg tgagtacgcg ccgggcgacg actttcgcca gatcgattgg
     1441 aatgcctacg cgcggctgga gcggttgttc ctgcggctgt tcgtcgagga agaagacgcc
     1501 accgtccatg tgatcgtgga tgccagccgg agcatggact ggggacagcc gctcaaatcg
     1561 acctacgctc ggcgtgtcgc ggctgcactg ggctacgtag ctctggtcgg gatggatcgc
     1621 ttggtcggcg cggcgcttgg actcgatggg cgatcctacg cgttgtttcc accccatcgc
     1681 ggcaagcatc aagccctggc gtggttcgac tggctggccg ggcttgccct gagcgaagcc
     1741 gaagggttgc agcctggcgg acaggcctcg cccctggccg ctctgacgca gtatgccgcc
     1801 agtgtgcgcc gcgccggtcc gctcgtcgtc gtcagcgatc tgatggatga cggctggcgc
     1861 gagggcatcc aacgcctggc cgcgcgtcac tacgagatca cgctgctgca catcctggct
     1921 ccccaggaaa tcgatcccga atgggaaggt gatctgaaac tggtcgacag cgagacgaat
     1981 gccgccgtcg agatcaccgc cgacttcgat ctgctgtcgc gttaccgcgc gcgcctggaa
     2041 gcctggcaga gcgagtggca gcagttttgc gccgcgcgcg cgatcaatta cgcgccgatt
     2101 gaaacgtcac tgccctttga ggagttagtg ttgtcgtttc tcaggcggcg ggggatattg
     2161 aagtgagggc tgtaccatga acttcattac tcctctcgcc tttggcctgg cggccctctt
     2221 gcccgtcatc gtcgcgctgt actttctgaa actgcgacgc caggaacagt ccgtttcttc
     2281 cacgtatctg tggcggacgc tcgtgcgcga tacggctgcc aacgcgccct ggcagcgcct
     2341 caagccaaac tggctgcttc tgctgcaact gttgtttctg atcgcactca tcgtcgcgct
     2401 ggcgcgcccg tttacgtgga gcgaagccgc ggccagctcg cacctcatcc tggtgatgga
     2461 cacttcggcc agcatgggcg ctacggacgt cgagccgaat cggctggcgg aagcggtgac
     2521 cacggctcgg gagctgatcg agggtttgcc gaattcgacg cgcgtgacgg tgatcgaggc
     2581 gggcgctcaa gtgcgtgtgc ctgtgtctgg cgcgagcgat ccgggtgcgg cagtcgccgc
     2641 actgagcgcg ctgcgccccg gctgggccgg cgccgatctg acatccgccc tgacgatggc
     2701 ggccgccatc gccgcccgcg aaccggacag cgaggtcgtg attctatcgg atgggcgcgt
     2761 caccctgccc gatgcttcca ccccttcgac ttcgctcagg gcaggcttca cgctgcccgg
     2821 tcgcgtccgc tacatcccca tcggtcgcga aaacgcaaac caggccattg gcgcattctc
     2881 gctgcaggcc gaatcgggca ggcgcagcct gacggcgttt gtgcagatgg tgaacttcgg
     2941 gagagaggat atgcagcgac gcctggtgtt gcgcgaagcg gccggcgggc ctgccccttc
     3001 gcaaggctca gggcaagctc tgagcggagc cgaagggcgc ttgctgacgg tgcgcgatgt
     3061 gacactgccg gcgggcaagt cccaggcgct cattctgccc ggcctgcccg gcgacgcgca
     3121 tgcggtcgag gcccgcctgg aaggtcagga tgtgttgtcc gccgacgacc gcgcctgggc
     3181 ggtgccaccc tcgaccgaga aagttgccgt gcgcattgtc gggccaggca atcgttttct
     3241 ggaaactgcg ctgcgcctga tgcccaacgt cgagatcacg ctggtgcagc ctgaccaact
     3301 atcagaccaa ctgtccaacc agccgaccaa ccaactcacc atattcgatt cagtcgttcc
     3361 gaccggcacg ctgcctggcg gtaatcttct gtttatcgca cctccgcgct cgaccgaagt
     3421 cttttccgtg acgggacgga tcgatgcgcc gctggccgcc cccgttgtcg ccgatgatcc
     3481 gcttttgcgc tacgtggatt tgcacgaggt ggctgtgcag gatgccgcgc gccttgcgct
     3541 gcccacctgg ggacgcgccg tcgtcctcga caccaagacg gacgcgccgc tgctcgtcgc
     3601 cggcgagcga gacggacgac gactggccgt gctggccttc gatctgcgtc gcagcgacct
     3661 gccgctgcgc gtggcctttc cgatcctggt cgccaacctg gtcgatgcgc tggtgcccgg
     3721 cggcgcagcg ggtgttccgg ccagcgtcga gccggggcag ccgctggtta tttctgcctc
     3781 gccgcaggcg agcgcaatcg tcgtgcgtac acccgatggc caatcccaca ccttgatacc
     3841 tgccgggggg agcgtcgtct tccagcagac cgatcggccc ggcgcatacg agatcgcggc
     3901 gcaagatagt gccggccagc tccagaacct gggcttgttc accgtcaacc tattcaatcc
     3961 gcaagagtct gacgtctcgc cgcgtgagac gttgcccgtg gctagcgccg gctcggtgag
     4021 cgcacaagag ttgccgcgcg ctcgagacga atggtggcga cctattgcct ggatggcttt
     4081 ggttttgctc gtggcggagt ggctgctggt ccatcgcggg aacctggcgc ggctctggag
     4141 ccttgtaacc gcgcgcggta gacccgccgg tgtgaagcgc aacgcgtgaa gggataaaga
     4201 tcactacccg tcccgtccgt gcatccgtgt cgagtccgtg tcgtgatgac cctttctttc
     4261 ctttacccga cagccctata cgcgcttctc ctgctgcccc tcttgttgac attgccattc
     4321 gtgggccggc gcgcctcggc gcgtacgttc aatttttggg tgggcctcgc gctacgcgcg
     4381 gtcgtcctgt tgggattgat cctgagtctg gccggcacgc aggtggttca ggcggtgaac
     4441 gataccacgg tcgtcttcgt ggtcgatcgt tccgatagcg tgccccgggc cgagcaaaag
     4501 cgcgccgagg attttgtccg tgcttcgctg gcgacgatga aagccggcga ccgcgccgcc
     4561 gtcgtggtct ttggcgagga ggcgctggtc gagcggttgg cttcggatga cacgacgctg
     4621 gcgcccatca cctcggcccc gcgtacgacc catacccatc tcgcttcggc gctgcgtctg
     4681 gcgctggcac tcttccccga agagacccac aagcgcatcg tgctgctttc cgatggcctg
     4741 gagaacgtcg gccacgccgc cgacctcgtc gacctggctg tagcgcgggg cgtgcaaatc
     4801 gccgtgatgc cgctggcgcc ggccagcggt cgggccgaag cctacctcga tgcgctcgaa
     4861 gcgccagcgg ccgttcgcaa ggggcagacg tttgagatcg tcgccgtgat ttacagcacc
     4921 agcgccggtc cggccagcct gcgcttgttc ggcgacggca aactgattgc gacgcgcgat
     4981 cttaccctgc agcccggcga caatcgcgtc agtttctcgc tcacgggcga cgagccgggc
     5041 tttcatcgct acacggcgga actccaggca gccggcgaca cgctgccgca gaacaacgtc
     5101 gcctcggcct tcagcgtcgt tcacggtccg ccgcgcgtcc tggtcgtcga gaacacttct
     5161 ggcgatgccg acaacctgat cgccgcgttg gaatcggcca aggtcgaaac ggaacgcgtc
     5221 gcgccggctg tcctcacggg tgacctggcg acgctgtcta actacgatgc ggtgttcctc
     5281 gtcaacatcc ccgccagagc attgcctgac gcggcaatga acgcgctccc gacttacgtg
     5341 cgagacctgg ggcgtgggct ggtgatgatc ggcgggccgg aggcttacgg cgcaggcggt
     5401 tacctgcgca cgcctctcga aaaggcgttg ccggtggata tggatgtgcg ctcgcggacg
     5461 caggaaccga atctggcgct ggtgtttgcg atcgataaat ctgggtccat gggtcgctgc
     5521 cactgtgacg atccgagcgc gcagcccggc agttatgccc gcatcgagag tgggctatcc
     5581 aaggtggata tcgccaagga tgccgtcatg caggccgcgc gtgctgtcgg ccgcgccgat
     5641 tacgtcggcg tggttgcctt tgacgcgaac gcgctgtggg cgctgcgcgt gcaggaaatg
     5701 atcgaccccg atttcgtcca gcaaaagatc ggcggcctgc gcgctgaagg gcagaccaat
     5761 atcaatgccg ggttgagcga agccgaagcc gcgctggagc agacgccggc gcgcctcaag
     5821 cacgtcgtct tgctcaccga tggctggagt cgctcatttg aatatggcgc gctggcgctc
     5881 aggctgcacg acaagggcat tactctatcc gtcatcgctg cgggcagcgg ttcggcgacc
     5941 tacctggagc aactggcgcg ccaggctggt ggtcgctatt acgcagcacg cacgatgacc
     6001 gaggtaccca gcctgttctt caaggagacg gtagaagccg ccggttcgta catcgtcgag
     6061 gagccgtttt accccgcgcc tgccggggcg acgccgatcc tgcgcgggct ggacgtcacg
     6121 cgcttgcccg cgctgcgcgg ttataacggg accacgccca agtcgagcgc acaggtgtcg
     6181 ctggtcagca gccagggcga tccgctcctg gcgacctggc agtatggatt ggggcgcgct
     6241 gcggcgtgga cctcggacgt caaggggcag tgggcgaccg actggatcaa gtggaatgga
     6301 ttcaacacct ttatcgcgca actgacgagt tgggtcttgc cgcagccagc cgacgaggga
     6361 ttgcaaacca tgttcgcgtc tgacggcgag cagaccgtgt tggaagtgac atcgaaggat
     6421 gcagacgggc gcccgcgcga tctgcttgac acgcatgcga cggtcgtcgg tcccgactcg
     6481 ccggcaggct ccggacaggc cctggtgtcg caagccatca cgctgaccca ggtcgcagca
     6541 ggccgctatc gcggccggtt gccggcggtg cagccgggca cgtatctggt gcgggtcacg
     6601 caacaggacg ccggtggcac gcccgtcgcc agcgcgacaa ccggtctgat cgtaccttat
     6661 tcgccggagt acaaatcgct gggcgaggca gccagcgtac tacctgagct gtctcgggcg
     6721 acgggcggac aagttctcgc cgagccggaa ctggcatttg cgcctctggc tcgaccggcc
     6781 tcgcgggcac aaccgatatg gccgacactg ctcttggccg cggcgctcct gttccccttg
     6841 gatgtcgcag cgcggcggct gcgtctcacg cgcggcgacg cgcagcgagt ttgggcgtgg
     6901 atgcgcacgc cacttcgacg gagaggggca gcggttgcgc cgcgcccgcg cgccttgagc
     6961 acattgtttg cggcgcgtga tcgagcgatc ggatcgcgct cccagagaat ggcgcagcct
     7021 gcttcgccgg tcgttgaagg cgagagacca tcacgacctc ctcctgaaag cagagagcca
     7081 tcacccgagc tgccggcttc gcctgaggcg atggccgcac gactcaggcg agccaaagat
     7141 cgggcacgca agtcgcgccc cgggtaaatg ctgggatacg agggttttgc ccacgccgag
     7201 gcgctgcgcc tggccggcca cactgccagg gcgcttgagc ggtactgcaa cttggccgag
     7261 acgagcgcct cactacattc tcattcatcc aatccacccc ggtaatcatc cctttcgacg
     7321 tcctcgcctt gccgaaatat tcggctggta taacgcagaa ctgtcggggc tgctcaagtc
     7381 agtattgtgc cgctcactcc tctatcactt gcgagcagcc tgctcagcat gtacaatgtc
     7441 tgacaactcg ccaatctcta atctccattc tctatgcaca aatacattct cttcgacctc
     7501 gacgaaaccc tctatccgcg cgacgccggg ctgatgcagg agatcggcgt gcgcatcttg
     7561 cgctacatgg tcgagcgcat gggtttcacg cccgaggcag cggaagcgaa gcggccgtat
     7621 tactaccaca agtacggcac gtcgctacgc ggactgatgg ccgaagaaac cgtcgatgcc
     7681 gaggattatc tgcgcttcgt gcacgaggtc gacatcacac gctacagcgg cccgaatccc
     7741 gagctggacg cgatgctgcg gcgtatcccg ctgtcgaaag tcgtgttcac caacgcgacg
     7801 cgagagcacg cccggcgcgt gctcgacgcg ctgggcatcg ccgatcattt cctgttcgtc
     7861 gtcgacgtgc gcgccatgga ctacgccagc aaacccgacc cgcaggccta ccggcgcatc
     7921 ctcgacgcga ttggcgcgcg cgccgaggaa tgtatcatcg tcgaagatac gccgcggaac
     7981 ctgctgccgg cgaaagcgtt gggcatgaag acgatcctgg tcgatcacca ggcgtgcgac
     8041 gaagtggact actgcgtgag cgacatcttg aaagtcggcg acgtggtggc gcgcctgctg
     8101 gcagatgata accaatgacc aatgaccaat aaccaattac tgataactga caaccgatga
     8161 ctgacaaact gatttccctc accgatgccg ctgcgctcgt ccagagtggc gacacgctgg
     8221 cgctcggcgg catgacgctg taccggcggc cggtcgcctt cgtgcgtgag ctgctcaagg
     8281 cggagacgca cgacctgacg ctgctgtgct tcacggcctc gtacgagagc gatctcttgg
     8341 tcggcgcagg gcgtgtgaag gcggtgcgca cgtgctactt cggcatggag gcgttcggcc
     8401 tggcgccgat gttcacgcag cgcgccacgt cgggcgattt gcgcgtgatc gaggagtccg
     8461 agggcagcct ggcgttcggc atgcgcgcga cgctggcccg cgtcggcttc atgcccgggc
     8521 cgggctgggt aggcacggac atgctcaaag tcc
//
LOCUS       DBNHXB010000220        17323 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3741151, whole genome shotgun sequence.
ACCESSION   DBNHXB010000220 DBNHXB010000000
VERSION     DBNHXB010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 17323)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17323)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17323
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3741151"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="AC162G_09070"
     CDS             <1..289
                     /locus_tag="AC162G_09070"
                     /inference="COORDINATES: protein motif:HMM:NF020967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR5076005.1"
                     /translation="SSEVDMFGPRNVKREGLEVVYRASGLLKAQVIKGRLEVSGIPAV
                     LDYDAVSQVFGITIDGLGEVRVMVPVERLEEARELIAAGDDEMVAGMDEEG"
     gene            complement(445..1767)
                     /locus_tag="AC162G_09075"
     CDS             complement(445..1767)
                     /locus_tag="AC162G_09075"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR5076006.1"
                     /translation="MTHRKKFTPRLSAFIRVLTIFIIVFAPIATARAQSAAPTIYVVQ
                     AGDTLFSIARRFSVPVNELARYNGVLNPDVIYVGQELKIPPTGQPSAASGTPTPVVSM
                     SAYRIHVVQSGENLFRIALRYGVTVEAIAAANRLGSTTLIFVGQQLSIPAPAGAPTDA
                     PVAQPKTPLPDPFVSFDVGPLPVSQGGIVVVKVHTREPVTLEGSFVDWRIPFAQEGDA
                     YYGLVGVSASPASGVQPGIYPLTITATQADGTQVAVSASVQVTSGRYNSETINLPPDR
                     EALLDPNLIAAEREKLNAVWTIFNSTRHWSGPFSLPIENYSKISSPFGTRRSYNGGPF
                     TSFHEGTDFSALTGTSVYAPANGVVALAEPLIVRGNAIVIDHGWGVYTGLYHLSSIDV
                     SVGQQVSQGDFIGRVGGTGLSTGPHLHWDIRVRSLNVDPMQLTRQALP"
     gene            complement(1764..2135)
                     /locus_tag="AC162G_09080"
     CDS             complement(1764..2135)
                     /locus_tag="AC162G_09080"
                     /inference="COORDINATES: protein motif:HMM:NF014678.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HIR5076007.1"
                     /translation="MKFVKVLNARGEPLLARTKWCPSFASRLRGLTFRRSLAPDEGLI
                     LVERRASVAATSIHMFFVNFAIAVVWLDDERRVVHKALARPWRPFYASPRPARYVLEG
                     PPALLERIAVGDILSFKETSG"
     gene            complement(2181..3221)
                     /locus_tag="AC162G_09085"
     CDS             complement(2181..3221)
                     /locus_tag="AC162G_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR5076008.1"
                     /translation="MNRRALRLILIAILSTVFAWRAAATSAQTSINLLQNPSFEEEYD
                     AWGDYRELRTAKGWTPWWIEFEGDEGHVLRRPECKPAHHFVFANRVKSGEFAQQCYTS
                     FGTHAMGVYQQVKATPGQVLRFSIWVQVWSTSLGDPSVSELPGNVRVSVGIDPTGGVD
                     PKSPVIRWAPDIEAYDRWHFQYIEEVAQNSVVTVFTRSIAEFAVRTNDIYWDEAGLVD
                     ADLALSLKLTPIPTATRVPTPRPGPDSILYAPPVTDTLRLIALRFGIDFARVAHANRV
                     PADVPVKPRFPVVVPGGQDEHAARGTYVVQPGETIYSIAARHQYSPIELAIINRRYGP
                     GIAWPGETLFLP"
     gene            complement(3343..4302)
                     /locus_tag="AC162G_09090"
     CDS             complement(3343..4302)
                     /locus_tag="AC162G_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloenzyme"
                     /protein_id="HIR5076009.1"
                     /translation="MTSILYVFLDGVGLGPDSDDNPLASARTPNLRAALGRPLIAGEA
                     ILRDGFLFRGIDATMGVDGLPQSATGQTALFTGVNAAQAVGMHVATYPTRPLRDLIAQ
                     HSLFRRAAEAGRRITFANPFHAVYWQAVEEGRARLSATTLAIKASGVPFRDLTDLERG
                     EAVFWDITHEIARLGRAPRRTRASRDGDSPRPAREYILSLPPITPGDAGRRLAALAAI
                     HDLTLYESFLTDLAGHRRLPIAPESAVETIDAFFGAVLAHRTPDTTLVLTSDHGNLET
                     RSFKGHTVNPVPLIVAGPAAAHFHDVRAITQVAPAILRALGCE"
     gene            4553..6718
                     /gene="glgP"
                     /locus_tag="AC162G_09095"
     CDS             4553..6718
                     /gene="glgP"
                     /locus_tag="AC162G_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HIR5076010.1"
                     /translation="MSPIHTSNRSFSLPKPISRLGELAYNLWWSWHPDAPDVFRWIDD
                     VLWRRVTHNPVKFLQLASRSRINAAIQDSGYMALYQHVLAEFNRYMEADDTWMQRTHP
                     GLINRPIAYFSTEFGLHESIPVYAGGLGVLSGDHLREASDLGLPLIGVGFLYREGYFR
                     QRVTEDGWQEAINEPLNLSELAILPEVDSEGRQVIVHVEMPGRVIQARVWRVQVGRVP
                     LFLIDTHVEVNDPADQSLSAQLYYSDLERRLQQEIVLGIGGVRALRTLGYNPAAWHMN
                     EGHSAFLTLERVAELVRSGRPFEEAREDIWRSTIFTTHTPVPAGHDKFPVWMMEKYFS
                     SYWSELGLDRDRFLSLALEAESWGDVFSMSLLAIRFAGQVNGVSELHGQVTRRMFARL
                     WPERSEDQIPIRSITNGIHTLTWLSRRMAALYEKHLGPDWREHLDDQAFWDHVRDIPD
                     QELWLVRRHLKRRLAAFVRERAREQWLRGGTHPVQIVAGGVLLDPYALTIGFARRFAT
                     YKRATLLLRDVDRLRRIVNNPARPVQFIFAGKAHPADDPGKHLIQDLYRVIKDAGMGG
                     RMVFLEDYDMNVARYLVQGVDVWLNTPRRPMEASGTSGQKAVVNGVLNCSVLDGWWRE
                     GYNGSNGWAIGDETQYGDPAEQDAADAESLFGTLENDVVPLYYERPYAGGAPAGWMRM
                     VKESIRTMLPMFGTNRMLKEYMAEMYVPAMKGEAVQTLP"
     gene            6917..7987
                     /locus_tag="AC162G_09100"
     CDS             6917..7987
                     /locus_tag="AC162G_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR5076011.1"
                     /translation="MSRPRKTRTTIHDVAKHAGVAKTTVSHAISGKRPVAPETRERIF
                     AAMRELNFRPNPVARRLAGGPGGAVAMVYPLASPSLSAVELRFINSIAEVVNRSDYMF
                     MNLSSPHVEIEQLHQIMHSGLVDGIILMRINMSDARVDLLKREGVPFVMIGRMADNQG
                     LSYVDLDAGAAIDIAIDHLVRLGHRTIAFVHPDDLDFGFAYRLVEGHRSACERRALPI
                     LAEPASLSDEAGYRAIPALLRRHSDVTAVIVWSDAVAVGVIRALQEHGRRVPDDISLI
                     SFDRSDQLRLISSELTIVDTRAEEVGARAAHMLLDVLEGRALDRRQVLVAPQLIVGQS
                     TGPCADGNGAAIAPAAVLTEGG"
     gene            8115..9437
                     /locus_tag="AC162G_09105"
     CDS             8115..9437
                     /locus_tag="AC162G_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5076012.1"
                     /translation="MKTRYMMLVSVLLVAAVLLTACPAPATTVAPTQPPAPQATEPPA
                     PQATEPPAPTEPAMEEVSFELWSQEGESEGVFQEIEALAEEYMAEHPNVTITVVQKDT
                     EALREDFQTASLAGAAPQLLWTVNDHAGVFVVADLIQPVDDLVDLDQFVDSALAAVEL
                     EGQHWGVPISNGNHLMLLYNKDLLAEAPADTDEMIAAAKELTSGDVYGLVYNATEPFW
                     LVPWLGGFGGVVFADDGKTPTLNTPEMIATLQFLHDLEFVEGIVPPEADYNAADTLFK
                     EGKAAMLINGDWSLGDYSNVLGDQLGVAPIPLVTATGEYPAPYTSGKFFMIAQDVEGA
                     QLEAILDFIQFTTSMDNQLRFTEKFRRLPALKAALDDPMIAGDPILAGSALQMENGTP
                     MPVQAEMRCNWDSMKPEMAKVLADEATPEDAAVAMQAAAENCVQTLQP"
     gene            9624..10901
                     /locus_tag="AC162G_09110"
     CDS             9624..10901
                     /locus_tag="AC162G_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5076013.1"
                     /translation="MEKLKPEVLGNSLQQVLSTVLVLVLVLVTFEVALYAGWRLAEWL
                     DKRLRKPSSAVAYVIQVGAFAVFFFVTLAIALSVLDSLDFKALLEPMEGDVVRGVYLW
                     AALSAIAIVPLVAFLGLVVSLIRLQRQPSLRLAFMLLAPAGIGLAALIGYPMYYELRL
                     AFSNMSLRNFREFQVGLEYGVANLQRLLTDPVLQQQTFTPVFARTVIWTAVNVFFHVC
                     GGLALALLLNRPMRGKGFYRTLLVLPWAVPQIIAVLAWRGEFHFQYGFVNLILTGLGL
                     EPINWLTDAVWNFVAMCLVNIWLGIPFMMVIFLGGLQSISGEYYEAAEIDGASPSQQF
                     RRITLPLLRPVMTPAIVLGTIWTFNNFNVPFLINQNELETSDILVTALYRAAFEYNRY
                     GFAAMFAFVIFLILLVFSLVYIWRSGTLKGVYE"
     gene            10898..11815
                     /locus_tag="AC162G_09115"
     CDS             10898..11815
                     /locus_tag="AC162G_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIR5076014.1"
                     /translation="MSTTTTVERPREVARPTAAAVSTFFRRTRGDSPFKRLLIHGTLI
                     LACLIAAYPILRVMSVALRPNDRLLSTSLEIVPPDASLDAFGEVLTNTDFLLWIWNSL
                     IITITTATIGVIIAATSAYAFSRWRFPGRSFGLVFLLATQMIPAAMLLVPLFILATQI
                     GIVNSYRGLVLAYSVTAVPFSIWILKGYYDTIPIDLEEAAKIDGASQMQAFVRILLPL
                     STPALAIVFLFNFMTAWNDYLLARVMVGSVADLNTWTLGFKHFIDQFRTDWGNFAAAS
                     IMVMIPVMALFLYSAKWLISGLTLGSVKG"
     gene            11829..12473
                     /locus_tag="AC162G_09120"
     CDS             11829..12473
                     /locus_tag="AC162G_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HIR5076015.1"
                     /translation="MRLLWDILKGAASDIAAEPYLFIVYNLLWVVMSLLIIPLPFATG
                     GLFFAAREVSARRAVGWRTFFVGGRQVARPMYLWGALNLAVALILITNISFYGRLGNV
                     FSDVIQSVLISAALVWLLLQLFVVPLLIQLRQPRLRVAVQDGSFLMLRHPLTTLAMAL
                     VVTVLIVASTMIPVLLAGITPAAIAVLSTRVVGEELARHRDEESAGRSLESKPK"
     gene            12470..15628
                     /locus_tag="AC162G_09125"
     CDS             12470..15628
                     /locus_tag="AC162G_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HIR5076016.1"
                     /translation="MNRTFENLVSLLALLAVIAAACTPPTETPAPPITPAEPTDTPVP
                     VEPEEPPLYVALVWHQHQPLYYKDPETNVYTRPWVRVHATKDYYDMAAILKSYPDVHV
                     TFNLTPVLIRQLDDLAAGAKDIYWVLAEKPADQLTEDEKRFILQRFFDVNPKIVNRFP
                     RYTELQAKRGGAEPEQIDAALESYTEQDFRDLQVLFNLAWFDPDFLTEEPLKSLVEKG
                     ESFAESDKPILFDKATEVIQSVIPVHKELQDAGQIEVITTPYAHPILPLIYSTRLALV
                     GSPEGEKPTDTFSYPNDAKAHVEKSVEIYEQHYGQKPRGMWPGEGAVAEDIVKMVGEA
                     GYNWMASGEQVLARSIGIDSFTRDSADTVQEADALYRPYLVEDAKSGAKVNMIFRDLR
                     LSDLIGFEYSNKDGEAAAEDLMQRLENIRQQLKKEGAEGPHLVSIILDGENAWEHYPN
                     DGKAFFHALYEKLSSTPAIKTVTPSEFFAMVPTERAIESLYPGAWFSPNYDTWIGESE
                     EAKAWDYLRRTRQTLAQYDILQRRTTSPENLARALDFMYLAEGSDWFWWYGADQDSGN
                     DDYFDFSFRSLLSEVYIALGELVPDFVQAPIVSRRAAQPTQPFKGLFTPIIDGAAAEG
                     EWDNAGTYLVSGGAQARSEDVIAALRYGMDQNNLYARIDARDSWGDIPEGAVNLYLSM
                     PRAEGSSPFSIRSAVAEDKTALGFQATHALDVSLDSGAATPYRANTIGEWIAGGAEGI
                     QVAVDGSVLEAAIPLALLGDDVQTGDDLRFAAIASSGLRDLHLVPTAGPAQVVIPDLG
                     TTTVVLSVADPEGDDHGPGTYTYPTDGVFKPGVFDIKEFTAGYDENNIVFKFTFYGPI
                     PNPWGSPNNLAVQTLDVYVDKDPGTGTGARLLLPGRNAALEPGNGWEVAIWAEGWTPQ
                     IHAPDAEGKPKQMSADFKVLVDPAAQTATLRVPRSVFGDDFDPARAAYLGIVASQDGF
                     PSKGVWRLRDVFQTAEQWKLGGGPDDANHTRIIDAAIPAGAPKSQEESLSAYPPSQET
                     NLDLLAPDDFPQLPALPASLGS"
     gene            15702..16832
                     /locus_tag="AC162G_09130"
     CDS             15702..16832
                     /locus_tag="AC162G_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076017.1"
                     /translation="MASVTYDHVTKRFGGGKQGDGKGVVAVRDLDILIADREFLVFVG
                     PSGCGKTTCLRMLAGLEEITEGQILISDRVVNDVPPKDRDIAMVFQSYALYPHMSVYD
                     NMAFALKLRKMPKAEIDQRVKRAADILGIGNLLDRKPKQLSGGQRQRVAVGRAIVREP
                     KVFLFDEPLSNLDAKLRVQTRAEIIKLHQQLGTTFIYVTHDQTEAMTMGTRIAVLKDG
                     VLQQCDTPQRLYDKPDNMFVAGFIGSPSMNFFDATIARGDGGLKAVGKEGRFEVPVPP
                     DRREPLQGYIDRTVVLGIRPEDIHDIAFTPPGIRSALIEAQVDVTELMGNEVFLYLIT
                     GGTNYVARVDPRTQARVGATIQVAANLDNIHFFDKETEKVLR"
     gene            17003..>17323
                     /locus_tag="AC162G_09135"
     CDS             17003..>17323
                     /locus_tag="AC162G_09135"
                     /inference="COORDINATES: protein motif:HMM:NF019924.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HIR5076018.1"
                     /translation="MKILFLAVECAPFYKVGGLADVIGSLPRALRELGHDVRVAIPRY
                     RSIDGAKHKLIRAGSTFSAYVGSEARRTEMLRSDASGVPTYFVWDERFFNRDVVYGQP
                     DEVMS"
BASE COUNT         3134 a         5338 c         5688 g         3163 t
ORIGIN      
        1 gagctcagag gtcgacatgt tcggaccgcg caacgtgaag cgtgagggcc tggaggttgt
       61 gtatcgcgcg tcgggcttgc tgaaggccca ggtgatcaag ggccgcctgg aggtctcagg
      121 tatccccgcc gtgctcgact acgacgccgt cagccaggta ttcgggatca cgattgacgg
      181 gctgggcgag gtgcgcgtga tggtgccggt cgagcgcctc gaagaggcgc gggagttgat
      241 tgcggccgga gatgacgaga tggttgccgg aatggatgaa gaaggatgag catatagacc
      301 tggcaggtct tgaggacctg ccaggtctgc ccataacgag gaacccggtc tctgccagag
      361 gccgggttcg ttcttttctc agggcgcgga gaaaaccttg aagttcttgg tgtctttgcg
      421 ccttggtggc tgcaatcagg actctcaagg tagcgcctgg cgcgtgagct gcatcggatc
      481 gacgttcagg ctgcgcaccc gaatgtccca atgcagatgc ggcccggtcg acaggccggt
      541 gccgccgacg cggccgatga agtcgccctg actcacctgc tggccaacgc tcacgtcgat
      601 cgacgacaga tgatacaggc cggtgtagac gccccagccg tggtcgatga cgatggcatt
      661 cccgcgcacg atgagtggct cggccagagc gacgacgccg ttagccggcg cgtacaccga
      721 tgtgccggtc agcgccgaga agtcggtgcc ttcgtgaaaa gaggtgaacg gcccgccgtt
      781 gtacgaacgg cgcgtgccga acggcgagct gatcttggag tagttctcaa tcggcagcga
      841 gaacgggccg ctccagtggc gcgtcgaatt gaagatcgtc cagaccgcgt tgagcttctc
      901 acgctcggcg gcaatcaaat tgggatcgag cagcgcctca cgatccggcg gcaggttgat
      961 cgtctccgag ttgtagcgcc cggaagtgac ctgcacgctg gccgacacgg cgacctgcgt
     1021 gccatctgcc tgggttgcag tgatggtgag cggatagatg ccgggctgaa cgccgctggc
     1081 cgggctggcc gagacgccaa ccaggccgta gtaggcatcg ccttcctgcg cgaacggaat
     1141 gcgccagtcg acgaacgacc cttcgagcgt gactggctcg cgcgtgtgca ccttgacgac
     1201 gacaatgccg ccttgcgata ccggcagcgg ccctacgtca aagctgacga acgggtcggg
     1261 caggggcgtc ttcggctgag caacgggggc atccgttggc gcgccggctg gggccggtat
     1321 gcttaactgc tggccgacga agatcagcgt cgtgctgccg agccgattcg ccgccgcaat
     1381 cgcctcgacg gtgacgccgt atctaagcgc gatgcgaaag agattctcgc cggactggac
     1441 gacgtggatg cgataagcgg acatcgacac taccggcgtg ggcgtcccgc tcgcagcgga
     1501 tggctggccg gtaggcggta tcttgagctc ctgccctacg taaatcacat cgggattcag
     1561 cacgccattg taccgggcaa gctcgttgac cggcacgctg aagcggcgcg caatgctgaa
     1621 gagcgtgtcg cccgcctgga cgacgtatat cgtcggcgca gcagattgcg cgcgagctgt
     1681 cgcgatcggc gcgaagacga tgatgaagat tgtaaggacg cggatgaacg cagataaacg
     1741 cggagtgaat ttcttacggt gcgtcatcct gacgtctcct tgaagctcaa aatatcgccg
     1801 acggcgatgc gttcgagcaa agcgggcggc ccttccagga cgtaacgcgc cggacgcggc
     1861 gatgcgtaga atggacgcca cggccgcgcc agcgccttgt gcacgacgcg gcgctcgtca
     1921 tcgagccaga cgacggcgat agcgaagttg acgaagaaca tgtgaatgct cgtcgcggcg
     1981 acgctggcgc gccgctcgac gaggatcagg ccttcgtcag gcgccaatga gcgccggaaa
     2041 gtcaagccac gcaggcggga cgcgaacgaa ggacaccact tcgtgcgcgc caggaggggc
     2101 tcgcctcggg cattaaggac tttgacgaat ttcatggcac tcaagaccta acaggtctca
     2161 aagacctgtt cggtctgcgt tcacggcagg aacaacgtct ctcccggcca ggcgatgccc
     2221 ggcccgtacc gccggttgat gatggctagc tcgatcgggc tgtattgatg cctggctgcg
     2281 atcgagtata ttgtctcgcc cggctgcacc acgtaggtgc cgcgtgcggc atgctcgtcc
     2341 tgcccacccg gcacgaccac cgggaaacgc ggcttcaccg gaacatccgc cggcacccgg
     2401 ttcgcgtggg ccacccgggc gaagtcgatc ccgaagcgca gggcgatgag ccgcaaagta
     2461 tccgtcaccg gtggcgcata caggatcgag tccggtccgg gtctcggcgt cggcacgcgc
     2521 gtcgccgtcg gaatcggcgt gagcttcagc gaaagcgcca gatcggcgtc gaccagaccg
     2581 gcttcgtccc agtagatatc gttggtccgc acggcgaatt cggcgatcga gcgcgtgaat
     2641 accgtgacga cgctgttctg cgcgacctcc tcgatgtact ggaagtgcca gcgatcgtaa
     2701 gcttcgatat ccggcgccca gcgaatgacg ggcgacttgg gatcgacgcc gcccgtggga
     2761 tcgatgccga ccgacacgcg cacgttcccc ggcagctcgg acacgctcgg atcgcccaga
     2821 ctggtcgacc agacttgcac ccagatggag aagcgcagca cctgccccgg ggtggccttg
     2881 acctgctggt acacacccat ggcgtgcgtg ccgaagctgg tgtagcactg ctgcgcaaac
     2941 tcgccggact tgacgcggtt ggcgaacacg aaatggtgag ccggcttgca ttcgggccgg
     3001 cgcagcacgt gcccctcgtc gccctcgaac tcgatccacc acggcgtcca ccccttcgcc
     3061 gtcctcagct cgcgataatc tccccaggcg tcatactcct cctcgaaact cggattctgg
     3121 agcaggttga tgctcgtctg cgccgaggtc gccgcagcgc gccaggcgaa tacggtggac
     3181 agtatggcga tcagaatcaa gcgcagcgct cgccggttca tcgctgcgga ttataccccc
     3241 ggtagggcgg ctttccatag ctgtcatttc gagggccggt aagaaacctg tcctaaggga
     3301 ctctgagggc aggtaagaaa cctgccctac gggactctga gactactcgc atccgagcgc
     3361 gcggagaatc gctggcgcga cctgggtgat tgcgcgcacg tcatggaagt gcgccgcggc
     3421 cggcccggcg acgatcagcg gcaccggatt cacggtgtgg cccttgaacg atcgcgtctc
     3481 gagattgccg tgatcgctcg tcaggacgag cgtcgtgtcg ggcgtgcgat gcgcgagcac
     3541 cgcgccgaag aaagcgtcga tcgtctcgac ggcgctttcg ggcgcaatcg gcagccgccg
     3601 gtgcccggcc aggtcggtga ggaaagattc atacagcgtc aggtcatgaa tggcggcgag
     3661 cgcggccagc cgccgcccgg catcgccggg tgtgatgggc ggcagcgaca agatatactc
     3721 tcgcgccggt cgcggcgagt cgccatcgcg gctggcgcgc gtgcgtcgtg gcgcccggcc
     3781 cagccgggcg atttcgtgtg tgatgtccca gaaaacggcc tcgccgcgtt cgaggtcggt
     3841 caggtctcgg aaagggaccc ccgatgcctt gatcgccagc gtcgtcgccg aaagcctggc
     3901 gcggccctct tcgacggcct gccagtagac cgcgtggaac ggattggcga aggtgatgcg
     3961 gcggcccgcc tcggcggcgc gcctgaacag gctatgctgc gcgatcaagt cacgcagcgg
     4021 ccgggtgggg taggtggcga catgcatccc aaccgcctgc gccgcgttga ccccggtgaa
     4081 cagcgccgtc tggccggtcg ccgactgcgg caggccgtcg acgcccatcg tcgcatcgat
     4141 gccccggaac aggaagccgt cacgcaggat cgcttccccg gcgatcaaag gccggccgag
     4201 cgcggcgcgc agattcggcg tgcgggcgga cgcgagcggg ttgtcgtcgg aatcggggcc
     4261 gaggccgacg ccgtcgagaa agacgtacaa gatcgacgtc atcgcagcga attctccgag
     4321 cagacgtggc aggtcttcgc aacgcggagc gagcctactg ctaacgttgg cgaacaagac
     4381 ctgccaggtc tatggctcga aatttccatt tgtggtcagt atagcatgcg gatccgatgt
     4441 gggcaatcgc aaaccatcgg ttggcgaaaa gttgatcgcc ggcagtcgca tgccttgacg
     4501 gttcggaagg tgggcattag aatcgacgca gcctataagg ggagaatgca cattgtcgcc
     4561 cattcatacc agtaatcgct ctttctcttt gccgaagccg atcagccggc tcggcgagct
     4621 ggcctacaac ttatggtggt catggcatcc ggacgcgccc gatgtgtttc gctggatcga
     4681 cgacgtgctg tggcggcggg tgacgcacaa tccggtgaag ttcctgcagc tggcgtctcg
     4741 atcccggatc aatgctgcga tccaggattc cggctacatg gcgctgtatc agcatgtgct
     4801 ggcggagttc aatcgataca tggaagccga cgacacgtgg atgcagcgga cgcatcccgg
     4861 tctgatcaac cggccgattg cgtacttctc gaccgagttc ggtctacacg agtcgatccc
     4921 ggtgtacgcc gggggcctgg gtgttctctc cggcgatcac ttgagagagg cgagcgacct
     4981 gggcctgccg ttgatcggcg tgggtttcct gtatcgcgag ggctatttcc ggcagcgtgt
     5041 cacggaagac ggctggcagg aggctatcaa cgagccgttg aatttgagcg agctggctat
     5101 cctgcccgag gtagactctg agggccgtca ggtcatcgtc cacgtcgaga tgccgggccg
     5161 cgtgattcag gcccgcgtct ggcgcgttca ggtcgggcgc gtgccgctgt tcctgatcga
     5221 cacgcacgta gaggtgaacg atccggccga tcaaagcctg tcggcccagt tgtactatag
     5281 cgatctggag cggcgtttgc agcaggaaat cgtgctgggc attggcggcg tgcgcgcgct
     5341 gcgcaccctg ggctacaacc ccgccgcgtg gcacatgaac gagggacact cggcgttcct
     5401 gacgctggag cgcgtcgccg agctggtgcg cagcgggcgg ccgttcgaag aggcgcgtga
     5461 agacatctgg cgctcgacga ttttcaccac acacacgccg gtgccggcag gccacgacaa
     5521 gttccccgtc tggatgatgg agaaatactt ttcgtcgtac tggagcgaac tgggcctcga
     5581 ccgcgatcgc tttctgtcgc tggcgctgga ggccgagtcc tggggcgacg tgttcagcat
     5641 gtcgctgctg gccatacgat tcgccggcca ggtgaacggc gtcagcgagc tgcacggcca
     5701 ggttacacgc cgcatgttcg cgcggctgtg gccggagcgc tcggaggacc agatcccgat
     5761 ccggtcgatc acgaacggta tccatacgct cacgtggctg tcgcggcgta tggcggctct
     5821 gtacgaaaag catctgggtc cggattggcg cgagcatctc gacgatcagg ctttctggga
     5881 ccacgttcgg gacatccctg atcaagaatt gtggcttgtg cggcgtcacc tcaagcgccg
     5941 gctggccgcg ttcgtgcgcg agcgggcgcg cgagcagtgg ctgcgcggcg gtacgcatcc
     6001 ggtgcagatc gtcgccggcg gcgtgctgct cgatccgtac gcgctgacga tcggcttcgc
     6061 gcgccgcttc gccacctata agcgggcgac gctgctgctg cgcgacgtcg atcgactgcg
     6121 gcgcatcgtg aataatccgg cccggcccgt gcagttcatc ttcgcgggca aggcgcaccc
     6181 ggccgacgac cccggcaagc atctgatcca ggacctgtac cgcgtgatca aggacgcggg
     6241 gatgggcggg cgcatggtct tcctggaaga ctacgacatg aacgtcgcgc gctacctggt
     6301 gcagggcgtg gacgtgtggc tgaacacgcc gcgccgccct atggaagcca gcggcacgag
     6361 cggccagaag gcggtggtca acggcgtcct gaactgcagc gtgctcgacg gctggtggcg
     6421 tgagggctac aacggcagca atggctgggc gatcggtgac gagacccagt acggcgatcc
     6481 ggccgagcaa gacgccgccg acgccgaatc gctgttcggc acgctcgaaa acgacgtcgt
     6541 cccactgtat tacgagcgcc cgtacgccgg cggcgccccg gcaggctgga tgcgcatggt
     6601 caaagagtcg attcgcacga tgctccccat gttcggcacc aaccgtatgc tcaaggagta
     6661 catggccgag atgtacgtgc cggcgatgaa aggcgaggct gtccagacac tgccctaatt
     6721 tccatatcaa acgccccgaa aaacttactc tcgctccgcg cgccgtcctc gacgcgggca
     6781 gtggccgccg gggacggcgg cttgagcact tgtggataat caccagaatc caggtcttga
     6841 ctagcaaacc ggtttgtgct attctgttgt tatcctccca aaccggtttg ctattccatc
     6901 gggcctggag taagtggtga gtcgcccacg caagacgcgt acgaccatcc acgacgtagc
     6961 gaagcatgcc ggtgtcgcca agaccacggt gtcgcacgcc attagcggca agcgaccggt
     7021 ggcgcccgag acgcgcgagc gcatttttgc cgcgatgcgc gagctaaact tccggccgaa
     7081 tcccgtcgcc cgccgcctgg ccggcggccc gggcggcgcg gtggcgatgg tctatcccct
     7141 tgcctccccc agcctgtccg ctgtcgagct gcgcttcatc aatagcatcg ccgaggtcgt
     7201 caaccgcagc gattacatgt tcatgaacct cagctctccg cacgtcgaga tcgagcagtt
     7261 acatcagatc atgcacagcg ggttggttga cggtataatt ctcatgcgga tcaacatgag
     7321 tgacgcgcgg gtcgacctcc tgaagcgcga gggagtcccg ttcgtcatga tcgggcgcat
     7381 ggcagataac cagggtctgt cgtatgtcga tctggatgcc ggtgcggcaa tcgacatagc
     7441 gattgaccac ctggtgcgcc tgggccatcg aaccattgct ttcgttcatc ccgacgacct
     7501 cgatttcgga ttcgcttatc gtttggtgga gggccaccgg agcgcgtgtg agaggcgagc
     7561 gcttcccatc ctggcggagc ctgcctcgct gtccgacgaa gccggctatc gcgccatccc
     7621 ggcgctctta cgccgccatt ccgatgtgac ggccgtgatc gtatggtcgg acgccgtggc
     7681 cgtgggcgtc attcgagcct tgcaggagca cgggcggcgc gtaccagacg acatttcgct
     7741 gatttcgttc gatcggtccg atcagttgcg gctgatctcc tctgaattga cgattgtcga
     7801 tacgcgggcc gaggaagtgg gtgcgcgcgc ggcccacatg ctgctggacg ttttggaagg
     7861 gcgcgcactc gatcgccgtc aagtgctcgt cgcgccgcag ctcatcgtcg ggcagagcac
     7921 cggcccgtgc gctgacggca acggcgcggc gatcgcgccg gctgccgtgc tgaccgaagg
     7981 ggggtgatat tccgcatccg atcctggtcc atcgtgcttc aaccgcgtcg ttttcacatg
     8041 cgctcacccg caggcagatg cctgaatcca taccgggtga agcagcgaga tactctctgg
     8101 aggagtaaga agacatgaag acccgataca tgatgcttgt aagcgtgctg ctggttgcgg
     8161 ccgtgctgct cacggcctgc ccggcgccgg cgacgacagt cgcaccgacg cagccgcccg
     8221 cgccgcaagc gactgagccg ccggcccctc aggctaccga gccgcccgcg ccgacggaac
     8281 ccgcgatgga agaggtgtcc ttcgagctgt ggtcgcagga gggcgaatcc gagggcgtct
     8341 tccaggaaat cgaggccctg gctgaagagt acatggcgga gcacccgaat gtgacgatca
     8401 cggtggtcca gaaggacacc gaggcgctgc gcgaggactt ccagacggcc agtttggccg
     8461 gggcggctcc gcagctcctg tggaccgtca acgaccacgc cggcgtgttc gtcgtagccg
     8521 acctgattca gccggtggac gatctggttg acctcgacca gtttgtcgat tcggcgctgg
     8581 ccgcggtcga gctggaaggc cagcactggg gcgtgccgat ctccaacggc aaccacctga
     8641 tgctgctata caataaggat ctgctcgccg aggcgcccgc ggacaccgac gagatgatcg
     8701 ccgcggctaa ggagttgacc agtggagacg tgtacggcct ggtttataac gcgaccgagc
     8761 cattctggct ggtgccgtgg ctgggcggct ttggcggcgt ggtatttgcc gacgacggca
     8821 agacccctac gctgaatacc cccgagatga tcgcgacgct gcagttcctg cacgatctcg
     8881 aattcgtgga gggcattgtc ccgcccgagg ccgactacaa cgcggccgac acgctgttta
     8941 aggagggcaa ggcggcgatg ctgatcaacg gcgactggtc gctgggggat tacagcaacg
     9001 tcctgggcga ccagctgggc gttgcgccta tcccgctggt gacggcgacc ggcgagtacc
     9061 ccgcgccgta cacctccggc aagttcttta tgatcgccca ggacgtcgag ggcgcccagt
     9121 tggaggcgat cctcgacttc atccagttca caaccagcat ggataaccag ttgcgcttca
     9181 ccgagaagtt ccgccgcctg cccgcgctga aggccgcgct ggacgatccc atgatcgccg
     9241 gcgacccgat cctggccggc tccgcgctgc agatggagaa cggcacgccg atgccggtgc
     9301 aggccgagat gcgctgcaac tgggattcga tgaagcccga gatggcgaag gtgctggcgg
     9361 acgaagcgac gccggaagat gcggccgtcg ccatgcaggc cgccgccgag aactgcgtgc
     9421 agacgctcca gccgtaacga gccgtgttcg agacgaggaa cggggacccc tccgttcctc
     9481 gtccgctaag aaactatctc aaaactggct tggagtggcg actcacttgc cccgtagcac
     9541 agcggagtgt ggtcggtcgc ttaagcaacg actgaagtca ttactcccat ccttttgtcg
     9601 gttgagaacg aaaggttcca ggcatggaaa aactcaaacc tgaagttctc ggaaacagtc
     9661 tccagcaggt gctcagcacc gtgttggtgc tggtgctcgt gctcgtcacg ttcgaagtcg
     9721 cgctgtacgc cggctggcgg ctggcggagt ggctcgacaa gcggctgcgc aagccgtcga
     9781 gcgccgtcgc ctacgtgatc caggtcggcg ccttcgcggt cttcttcttc gtgacgctgg
     9841 ctatagcgtt gtccgtgctg gactcgctcg acttcaaggc tttgcttgag ccgatggagg
     9901 gcgacgtcgt gcgcggcgtc tatctgtggg cggcgctgag cgcgatcgcg atcgtcccgc
     9961 tggtcgcatt tctcggactg gttgtcagcc tgattcgctt gcagcgccaa ccgtcgctgc
    10021 gcctggcgtt tatgctactg gcaccggcgg gtatcgggct ggcggcgctc atcggttatc
    10081 ccatgtacta cgaactgcgg ctcgcgttca gcaacatgag cctgcgcaac ttcagagagt
    10141 ttcaggtcgg gctggaatac ggcgtggcga acctgcagcg gctcctcacc gatcctgttc
    10201 tccagcagca gacgttcacc ccggtcttcg ctcgtacggt gatctggacg gcggtgaacg
    10261 tcttcttcca cgtgtgcggc ggcctggcgc tcgctctgct gctcaatcgc ccgatgcgcg
    10321 gcaaaggttt ctaccgcacg ctgctggtgc tgccctgggc ggtgccgcaa atcatcgccg
    10381 tgctggcctg gcgcggcgag ttccacttcc agtacggctt cgtgaacttg atcctgaccg
    10441 ggctggggct ggagccgatc aactggctga cggacgccgt gtggaacttc gtcgccatgt
    10501 gtctcgtcaa catctggctg ggcatcccgt tcatgatggt catcttcctg ggtggcctgc
    10561 agagcatctc gggcgagtat tacgaggcgg ccgaaatcga cggggccagt ccctcccagc
    10621 agttccgccg catcaccctt ccgctgctgc ggccggtgat gacgccggcg atcgtgctcg
    10681 gcacgatctg gacgttcaac aacttcaacg tgcctttcct gatcaaccag aacgagctgg
    10741 agacgtcgga tattctcgtc acggcgctgt accgcgccgc gttcgagtat aaccggtacg
    10801 gcttcgccgc gatgttcgcg ttcgtcatct tcctgatcct gctggtcttc tcgctcgtat
    10861 acatctggcg ctcgggcacg ctgaaaggag tctacgaatg agcaccacta ctaccgtcga
    10921 acgccctcga gaagtcgcgc ggccgacagc ggccgctgtc tcgaccttct tccgccgcac
    10981 gcgcggcgac agcccgttca agcggctgct gattcacggg acgctgatcc tggcgtgcct
    11041 gatcgccgcc tatccgattc tgcgcgtcat gagtgtggcg ctgcgcccga acgaccgcct
    11101 gctgtcgaca tcgctggaga tcgtcccgcc agatgcctct ctggatgcgt tcggcgaggt
    11161 gttgacgaac acggatttcc tgctgtggat ctggaacagt ctcatcatca ctatcacgac
    11221 ggcgacgatc ggagtcatca tcgcggccac gtcggcgtac gccttctcac gctggaggtt
    11281 ccccggccgg tcgttcgggc tggtgttctt gctggcgacg caaatgatcc cggctgccat
    11341 gctgctcgtg ccgctgttca tcctggcgac gcagatcggg atcgtgaatt cgtatcgcgg
    11401 cctggtgctg gcctactcgg tgacggcggt gccgttcagc atctggatcc tcaaaggcta
    11461 ctacgacacg atcccgattg acctggagga agcggccaag atcgacggcg cgtcgcaaat
    11521 gcaggcgttc gtccgcatct tgctgccgct atcgactccg gcgctggcga tcgtcttcct
    11581 gttcaacttc atgaccgcct ggaacgacta cttgttggcg cgggtcatgg tcggcagcgt
    11641 ggccgatctg aacacctgga cgctgggctt caagcacttc atcgatcagt tccgcaccga
    11701 ctggggcaac ttcgccgcag catcaatcat ggtcatgatc ccggtgatgg ccttgttcct
    11761 gtattcggcg aagtggctga tctccggcct gacactcgga agtgtaaagg gatgataggg
    11821 tttgcagcat gcggctgctg tgggatatct tgaaaggcgc ggcgtccgac atcgccgccg
    11881 agccgtacct gttcatcgtg tacaacttgt tgtgggtggt gatgagcctg ctgatcatcc
    11941 cgctcccctt cgcgacgggc ggcctgttct tcgcggcgcg ggaagtcagc gcgcggcgag
    12001 ccgtcggctg gcgaacgttc tttgtcggcg gccggcaggt cgcccggccg atgtacctgt
    12061 ggggtgcgct gaacctggcg gtggccctga tcttgatcac gaacatcagt ttctatggcc
    12121 ggctggggaa cgtcttcagt gatgtgatcc agtccgtgct catcagcgcc gccctcgtct
    12181 ggctgctgct tcaactgttc gtcgtgccgc ttttgatcca gctcaggcag ccgaggctgc
    12241 gcgtggcggt acaggacggc tcgtttctga tgctgcgcca tccgctgacg accctggcga
    12301 tggcactggt cgtcaccgtg ctgatcgtcg ccagcacgat gataccggtt ctgctggccg
    12361 ggattacccc ggcggcgatt gccgtgttga gcacgcgggt cgtcggcgag gagctggcgc
    12421 ggcatcggga tgaggaatcg gccgggcgaa gcctggagtc caaacccaaa tgaatcgaac
    12481 gttcgagaat cttgtctcac tgttggcgct gcttgcggtg atcgcggcgg catgcacgcc
    12541 gccgaccgaa acgccagcgc cgccgatcac gccggctgag ccaactgaca cgccagttcc
    12601 ggtcgagccg gaagagccgc cgctatatgt cgcgctcgtc tggcatcagc accagccgct
    12661 gtattacaaa gacccggaga cgaacgtcta cacccggccg tgggtgcgcg tccacgcgac
    12721 caaagactac tatgacatgg ccgcgatcct gaagagctac ccggacgtgc acgtgacgtt
    12781 caatcttacg ccggtgctca tccgccagtt ggacgatctc gcggccggcg cgaaggacat
    12841 ctactgggtg ctggccgaga agccggccga tcaactgacc gaagacgaga aacgcttcat
    12901 cctgcagcgc ttcttcgacg tgaatccgaa gatcgtcaat cgcttcccgc gctacacgga
    12961 gttgcaggcc aagcgcggcg gcgcggagcc ggagcagatc gacgcggcgc tggagtcata
    13021 caccgagcag gacttccgcg atttgcaggt gctgttcaac ctggcctggt tcgatccgga
    13081 tttcttgacc gaagagccgc tcaagtcgct ggtggagaag ggcgagagct tcgccgagtc
    13141 ggacaagccg attctgttcg acaaggccac cgaagtcatc cagagcgtta tcccggttca
    13201 caaagagctg caggacgccg ggcagatcga ggtcatcacc acgccgtatg cccatccgat
    13261 cctgccgttg atctactcga cccggctggc gctggtgggc agccctgagg gcgagaaacc
    13321 gaccgacacg ttctcttatc cgaacgatgc gaaggcgcat gtcgaaaaga gcgtggaaat
    13381 atacgagcag cattacgggc agaagccgcg cggcatgtgg ccgggcgagg gcgcggtggc
    13441 cgaggacatc gtcaagatgg tcggcgaggc aggctacaac tggatggcct ccggcgaaca
    13501 ggtgctggcc aggtcgatcg ggatcgattc gttcacgcgc gacagcgccg acacggtgca
    13561 ggaagccgac gcgctctacc gcccctacct cgtggaggat gcgaagtcgg gcgcaaaagt
    13621 caacatgatc ttccgcgatc tgcgcctgtc ggacctgatc ggcttcgagt actcgaacaa
    13681 ggatggcgag gccgcggccg aagacttgat gcagcgcctg gagaacattc gccagcaact
    13741 gaagaaggaa ggcgcggaag ggccgcacct ggtgtcgatc atcctcgacg gcgagaacgc
    13801 ctgggagcat taccccaacg acggcaaagc gttcttccac gcgctgtacg agaagttgtc
    13861 gagcacgccg gcgatcaaga ccgtgacgcc gtcagagttc ttcgcgatgg ttccgacgga
    13921 acgcgcgatc gaatcgctgt atccgggcgc gtggttcagc ccgaactacg acacctggat
    13981 cggcgaatcg gaagaggcca aggcgtggga ttatctgcgc cgcacacggc agacactggc
    14041 ccagtacgac atcctccagc gcaggacgac gtcgcctgag aacctggcgc gcgcactcga
    14101 tttcatgtac ctggccgaag gctccgattg gttctggtgg tacggcgcgg atcaggacag
    14161 cggcaacgac gactacttcg acttctcgtt ccgctcgctg ctgagcgaag tatacatagc
    14221 gctgggtgag ctggtccccg acttcgtgca agcgcccatc gtttcgcgcc gtgccgccca
    14281 gccgacgcag ccgttcaagg gactgttcac cccgataatc gacggtgccg ccgctgaagg
    14341 cgagtgggat aatgcgggca cctatctcgt tagcggcggt gcgcaggcgc gatcggagga
    14401 tgtgatcgcc gcgctgcgtt acggcatgga ccagaacaac ctctacgcgc gcatcgacgc
    14461 cagggacagt tggggcgaca ttcccgaggg cgcggtaaac ctgtacctgt caatgccgcg
    14521 cgctgaaggc tccagcccgt tctcgatccg cagcgccgtt gcggaagaca agacagcact
    14581 cggcttccag gcgacgcacg cgctcgacgt gtcgttggac agcggcgcgg ctacgccgta
    14641 ccgcgcgaac accatcggcg aatggatcgc gggtggagca gagggcatcc aggtggccgt
    14701 ggacgggtcg gtgctggagg cggcgatccc gctggcgctg ctcggcgacg acgtgcagac
    14761 cggcgacgac ctgcgctttg ccgcgatcgc cagctcgggc ctgcgcgatc tgcacctggt
    14821 gcccacggct ggaccggcgc aggtcgtcat ccccgatctc ggcacgacaa ccgtcgtgct
    14881 gagcgtcgcc gatcctgaag gcgacgatca tggccccggc acgtacacct atccgaccga
    14941 cggcgtattc aagccgggcg tgttcgacat caaggaattc accgccggtt acgacgagaa
    15001 caacatcgtg tttaagttca cgttctacgg gccgattccg aacccgtggg gatcgccgaa
    15061 taatctcgcg gtgcagacgc tcgacgtgta cgtggacaaa gaccccggca cagggaccgg
    15121 cgcacgcctg ctgctgccgg gccgtaacgc ggcgctcgaa cccggcaatg gctgggaggt
    15181 ggcgatctgg gccgaaggct ggacaccgca gattcacgca cccgacgccg agggcaagcc
    15241 gaagcagatg agcgccgatt tcaaggtcct ggtcgatccg gcagcgcaga ccgcgacgct
    15301 gcgcgtgccg cgctcggtct tcggtgacga tttcgatccg gccagggcgg cctacctcgg
    15361 cattgtggcg agccaggatg ggttcccgtc caagggcgtg tggcggctgc gcgacgtgtt
    15421 ccagacggcg gagcagtgga aactcggcgg cgggccggac gacgcgaacc acacgcgcat
    15481 catcgatgcg gcgattcctg ccggcgcgcc gaagtcgcag gaggagtctc tgagcgcgta
    15541 cccgccgtcg caggaaacga atctcgacct gctcgcgccg gacgattttc cgcagttgcc
    15601 ggcgctgccg gcaagcctgg ggtcgtgacg gcgagtattg acacgaataa ccggtgatgc
    15661 tacactaggc acagcgtgcc cccacagaga ggagtgaacg catggcgagt gtaacctacg
    15721 atcatgtcac caagcgcttt ggcggcggca agcaaggcga cggtaagggc gtcgtcgccg
    15781 tcagggacct cgacattctc attgccgatc gcgaattcct cgtctttgtc ggcccgtcag
    15841 gctgcggcaa gacgacctgc ctgcgtatgc tggccgggct ggaggagatc accgagggcc
    15901 agatcctgat cagcgaccgg gtggtcaacg acgtgccgcc gaaggatcgc gacatcgcga
    15961 tggtctttca gtcatacgcg ctgtatccgc acatgtcggt gtatgacaac atggccttcg
    16021 cgctgaagct gcgcaagatg cccaaagccg agatcgacca gcgcgtgaag cgggcggcgg
    16081 atattctggg catcggcaat ctgctcgacc gcaagccgaa gcaactgtcg ggtgggcagc
    16141 gccagcgcgt ggccgtgggg cgggcgatcg tgcgcgagcc aaaggtgttc ctcttcgacg
    16201 agcctttgtc caacctggac gccaagctgc gcgtgcagac gcgcgccgag atcatcaagc
    16261 tgcaccagca gctcggcacg acgttcatct acgtcactca cgatcagacc gaagcgatga
    16321 cgatgggcac gcgcattgcg gtgctgaagg atggtgtgct gcagcagtgc gacacgccgc
    16381 agcgcctgta tgataagccg gacaacatgt tcgtcgccgg cttcatcggc agcccgagca
    16441 tgaacttctt cgacgccacg atcgcgcgcg gcgacggcgg gctgaaagcg gtgggcaagg
    16501 aggggcgctt cgaggtgccg gttccgcctg atcgccggga accgctgcaa ggctacatcg
    16561 acaggacggt ggtgctgggt atccgccccg aagacatcca cgatatcgct ttcacgccac
    16621 cgggtattcg ctctgcgctg atcgaggcgc aggtggacgt gacagagttg atgggcaacg
    16681 aagtgttctt gtacctgatt acgggcggca ctaactatgt agcgcgcgtc gatccgcgca
    16741 cgcaggcacg tgtgggcgcc acgattcagg tcgcggcgaa tctcgacaac atccacttct
    16801 tcgacaagga gaccgagaag gtccttcgat agcgatgctg aacgattgaa gccctcggct
    16861 actcgccggg ggcttcttct tgttcggcgc gggcctcggt cgggagacct tcggccatcg
    16921 gagagatggc cggcgcgcgt tgactcaccc cgtaggctgt gctagactat cactaccatc
    16981 accaccgcca gcagcgaaat tcatgaagat cctcttcctc gcagttgaat gcgcgccgtt
    17041 ctataaggtc ggcggcctgg ccgacgtaat cggctcgctg ccgcgcgcgc tgcgcgagct
    17101 ggggcacgac gtgcgcgtgg cgatcccacg ctaccgctcg atcgacggcg cgaagcacaa
    17161 gctgatccgc gccggatcga ctttcagcgc ctacgtcggc agtgaggcgc gccgcacgga
    17221 gatgctgcgc tccgacgcat ccggcgtgcc cacttacttc gtctgggatg agcgattctt
    17281 caatcgggac gtggtctacg gccagcccga cgaggtcatg tcg
//
LOCUS       DBNHXB010000221        12169 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2231708, whole genome shotgun sequence.
ACCESSION   DBNHXB010000221 DBNHXB010000000
VERSION     DBNHXB010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 12169)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12169)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12169
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2231708"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..894)
                     /locus_tag="AC162G_09140"
     CDS             complement(82..894)
                     /locus_tag="AC162G_09140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR5076019.1"
                     /translation="MRDLHIIVCMKVVPKPEEIRVDRETRRLERANARSEINPPDMNA
                     LEMALSLTDRYAGRVSILSMGLPFFEAYLRIGLAMGADHVYLLSDRAFSGADTLATTY
                     TLAKGIQKMGGADLIICGEESSDGATGQVPAGLAEWLGLCQLTQVNEVDVDPRRWIAR
                     GHRVIRGGYEILEVPLPAVVSVKTACNEPRFMDYRIKPWAFGPAGVTVWTEADLGVDE
                     ACIGAVGSPTVVSRLEQAPSRERKRVSVTGTPEEIARQLVELLGREAFVVQA"
     gene            complement(915..1952)
                     /locus_tag="AC162G_09145"
     CDS             complement(915..1952)
                     /locus_tag="AC162G_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR5076020.1"
                     /translation="MPASLYSADQAHNAIWVFLEQEGGILERVSLELLSKGRQLANQV
                     GWRLTGLLMGHGVSDQVEQAIAFGADEIVLAEHPLLDVFTVDAYGHVAFQAIMESKPS
                     IFLLGATPNGRDLAGRLAVRLRTGLNADCTDLRLNPETGVLVSEVSGFGGGVLALIEM
                     QKHRPQMATVRPGVFPPEPADYSRKGTVVRLPVNLTGAIIRTRIVERVVGQAADLTQA
                     RALVIGGRGVGGHFDMLRELAGLIGGEVGATRPPVDEGHIERDCQIGQTGVVCRPQVA
                     IVCGASGAFHFVVGIQKAGTVIAVNSDPEAPIFESADYCVVGDFRQIVPALMQQIREW
                     RAEQIGSTAGS"
     gene            complement(1968..3179)
                     /locus_tag="AC162G_09150"
     CDS             complement(1968..3179)
                     /locus_tag="AC162G_09150"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR5076021.1"
                     /translation="MRDEEAVVIDDARWEEIIELTGGAAAACYQCGVCTAICPWGLVK
                     KETVSVRHLLRRAQLGLPTGDDHPAGRGASTGASSLWLCTTCAQCEAYCPRGVAITDV
                     FRSLRAIAWERRQTPRGLPPLLWSVYWNNNPWSQPPSQRSAWTKGMHVPRYDAHRHEM
                     LLYVGCTSSYDGRARKVARSLTRLLDAASLSFGILGDDEPCCGEAVLSVGHKPYFAEI
                     AQQAAHVFHEKGVTRLVAISPHCYDAFKNHYPRGEEEFEPRHYTQVLATLVDQRRLVF
                     DRPVERKIAFHDPCYLARHNHECEAPRRILQAIPGLELVEMEHTGTETLCCGGGGGRM
                     WMETDAGERFSNIRVNEALASGADILVTACPFCIACLEDSLKAQGIRKLVVMDVAEIA
                     ALALTEKRERE"
     gene            complement(3176..6745)
                     /locus_tag="AC162G_09155"
     CDS             complement(3176..6745)
                     /locus_tag="AC162G_09155"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR5076022.1"
                     /translation="MGDSVLVIGGGIAGIQASLDLAEAGARVVLVEREATIGGIMAVL
                     DKNFPTLDCSICIEAPKMSEVGLHPNIEVLSLAEVESIEGGPGDFCVRIRQRGRYVTD
                     GCTRCGECTAACPVVLPNEYDAGMAVRKAIYTPIPQSVPGAYVIDIEHCLNDPPNYLP
                     CNRCDEVCPPKAIDFMMPRGCLLTRQVGSIIVAIGYDTLDPRKLKEYGYGTHADILTA
                     LEFERLISSAGPTGGEILRPSDGKHVDSILVVLCVGLRDRRFFRYCSRFCCMYSIKHA
                     YQAIDHGIPEVTVLYMDIRAYGKGFDDFWSRTRESGAKFIRGRPGRIQPVGDRIRVVY
                     EDTESATRIEAEYDMVVLATAVTPADGLARLAERLGIDVDEDGFIRAADTGGSPILTS
                     RPGIYAAGCASGPKDIPDSVSEGSGAAAIALSHLTHRSWPEPPEACPAPRSTLHASVG
                     EPGQRVEPMADIGTSRVGVFVCHCGSNIAGVVDVKQVVGFARTFPDVVYAADQMFSCA
                     GNTQSEIEEAIKRESINRVVVAACSPKTHESIFRGVLIRAGLNPYLLEMANIRNMDSW
                     VHKFDKAAATTKAMDMVSMAVEKARRLAPLQIGHLPLTQSVLVIGGGIAGMTAAATLA
                     RQGFETDLVERNEHLGGLLTQLDRIAPGGPSAALRACPDPDPERSEGEGEGAGLNARD
                     LVAAKSRELAQAGVHIHSGTIVESVGGVVGNFHASLSDGAEIRIGAIIVATGAAPYQP
                     REFGCGDDPRVITNLELENLMAKCRVQAERITFVSCVGSRQGDRGCSRYCCTSMINQA
                     LRLRQLGKKVHVISKDIRTYSRHAEELYEQAMRAGVRFFRYDGDRPPQDAIRFLNPEG
                     NELEWGVRVGGQVCFYDHLLGTNLRIPTDLLVLVVGLAPAEDGLSDQLKLAHSADGFL
                     MELHPKLGPAETATQGVFLAGAAQGPKDVRESIAQAMAAAGKAGALLSRGEIEKAPLT
                     ARVNLDLCIGCMRCVKVCPFSAIEQTGPVKEGRVRIVEAACMGCGTCAAECNFDAIDM
                     PTFTKEQILAQIDAALAEKPEEKCLVFTCNWCSYAGADLAGIEKRQYPTSARIIRTMC
                     SARLEEDFIARGFEKGAGAILVTGCRLTDTGSDCHYNYANRHTLKRFNHWHRKYTRQG
                     ITPERLQLQWISAAEGKAFAEKIHEMDAVVQQQARVVTMRDDG"
     gene            complement(6738..7298)
                     /locus_tag="AC162G_09160"
     CDS             complement(6738..7298)
                     /locus_tag="AC162G_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR5076023.1"
                     /translation="MRKEIRISGFGGQGVVLAGTILGKALSLHAGLEAVMTQAYGPEA
                     RGGASSADIVVSDEAVDYPFVEHPDILVALSQEAYTRFRPAAPFDALVLIDEDLVIPL
                     PDDHPHRIPATGMAETLGGRIMANMVMLGFFTGVTGLVDREAMREAIESSVKAQTAPL
                     NLQAFEVGFEHARPALSEVEGNKCHG"
     gene            complement(7295..8125)
                     /locus_tag="AC162G_09165"
     CDS             complement(7295..8125)
                     /locus_tag="AC162G_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5076024.1"
                     /translation="MGKALVANRHPLDDIIRSERMPHIWCPGCGIGIAMRCYARAILE
                     SGIPVDRHVVVSGIGCSGRAAGYMNIDSYHSTHGRAIPFATGLKLARPDLMVSVFSGD
                     GDLAAIGGNHLIHAARRNVDIKVICINNFNYGMTGGQLGPTTPFHARTTTSPYGNPEL
                     PFNLPYLLAAAGANHVSRWTTLHVRQVKNDILYAFDKPGFAFIEILSPCPVGFGKSNN
                     INEGVDEMELYRSRCVLADGVPMEQLGIDLREERPIYIGNFIDRDQPPYKPVRLEPAA
                     "
     gene            complement(8103..9323)
                     /locus_tag="AC162G_09170"
     CDS             complement(8103..9323)
                     /locus_tag="AC162G_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR5076025.1"
                     /translation="MKAHGVLTGKHFMHGDHACAEGAIAAGCRFFAGYPITPSTEIAE
                     RLARRLPEVSGVFIQMEDELGSLAAVIGASAAGTRAMTATSGPGFSLMMENLGLAVMM
                     EVPCVLVDVQRGSPSTGLPTMAGQSDVMQARFGSHGDYEIVAYSPWSPQEMFDLTILS
                     FNVADRYRVPVLLMSDEVIGHMVERVVIPPEDQIPRWERKRPGGTPNGHFDSFRAEDS
                     DPSTGSELALSLSKGLALVPPMAHAGEGYRVHFTGLTHDERGYPAMSAETHHRLVTRL
                     AEKVRRSADQLILTEGYQLDDARIVVVSYGCTARSTRRAVREARGKGIPVGLLRLVSL
                     WPFPEQTVKELAGQADSFIVAEMNLGQMIREVERHVKQPVRGVFHAGGAMIPPEPILD
                     AIQEVAACGQSVSR"
     gene            complement(9320..9589)
                     /locus_tag="AC162G_09175"
     CDS             complement(9320..9589)
                     /locus_tag="AC162G_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5076026.1"
                     /translation="MKAYGRTPLNLHQTRIPRGQVYLIPERCKGCKLCIQFCPQQVLQ
                     ESETANGKGYHYPEVTPGKEESCVHCEFCAMVCPEFAIYTLEVAQ"
     gene            10165..11187
                     /locus_tag="AC162G_09180"
     CDS             10165..11187
                     /locus_tag="AC162G_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007143367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR5076027.1"
                     /translation="MNILAEGQGELIGWHDPDENREWILENKPRDLRDKRTSVSDAVT
                     RLVQDGDFIAFGGFGHIRIPMALVYEIIRQRRRNLVMAGKTAVHDVDLLIGAGCVNRI
                     EVAYAFGHELRGLSPAGRRAVETGQCRVVAEISNAGYQWRFLAGMMGIPFIPSRNMLG
                     TDTFKHSACKVATDPWSGKPVCLIPAAYPDVAMFHVPRCDMYGNAQIDGIIVEDFELA
                     RAARRVIITAEEVVDEAIIRNQPCRTVIPFYVVDAVCEVPHGAHPTLMPYLYFFDEEH
                     IGEWLELSQTPEGAAAYFDKYVFGVCCFEEYLERIGGIRKMHYLKQVEQLKAPLRAPW
                     LKTRKG"
     gene            11194..11970
                     /locus_tag="AC162G_09185"
     CDS             11194..11970
                     /locus_tag="AC162G_09185"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR5076028.1"
                     /translation="MAAQVLEYTQTELLSAVAAHLLEDKKSVLVGTGLPMIAALLAQR
                     MHAPGLLIIFEAGGIGPRVPTLPISVGDSRTFYRAAAASSMHDVMSACQAGYVDYGFL
                     GAAMIDQYGNINTTVIGDWEQPAVRLPGSGGANDIGSLCHRTIIILRQDERKFVERVN
                     FITTPGYLDGPGARERAGLPYGSGPYRVITQLGVYGFDDETKRLTLLAMHPGVTRDDV
                     RANSSCEVLIPRVVDTTAPPTEEEQRLLREIDPAGMVIGR"
     gene            11971..>12169
                     /locus_tag="AC162G_09190"
     CDS             11971..>12169
                     /locus_tag="AC162G_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076029.1"
                     /translation="MRRAAFQSRWGERAFSIAFRSCAIPGLTLVAMGIVHLQLESPNV
                     AGAVAVPGDRQVSGAGGSREQT"
BASE COUNT         2214 a         3851 c         3780 g         2324 t
ORIGIN      
        1 tacgactagc ctgactttcg cgccatcagc gcccgaatcg ttgaacggga tgtcacgcga
       61 cctcggccat cgcatggtcg cttacgcctg gacaacaaac gcttcacgcc cgaggagttc
      121 cactaactgg cgtgcgatct cctctggagt gcctgtcaca gatactcgtt tccgctcccg
      181 cgagggcgcc tgttccagtc tcgaaacaac tgtcggcgat cccacagcgc cgatgcaggc
      241 ctcgtccacg ccgagatcgg cttcggtcca cacggtcact ccagccggcc caaaagccca
      301 gggcttgatg cgatagtcca tgaagcgtgg ctcattgcag gcggtcttca ccgaaaccac
      361 cgcaggcaaa ggcacttcca agatttcgta tccgcccctg atcactcggt gtccacgggc
      421 aatccatcgc cttgggtcga catccacctc attcacctgg gtgagctggc agagtcccaa
      481 ccattcggcc aggccggcgg ggacttgtcc tgtcgcgcca tcggacgact cttcgccgca
      541 gatgatgaga tcggcgccgc ccatcttctg gatgcctttc gccagcgtgt aagtcgtcgc
      601 cagcgtgtcc gccccactga acgcccggtc gctgagcaga tagacgtgat cggcgcccat
      661 cgccaagccg atccgcagat aggcctcgaa gaacggcagc cccatggata gaatgctgac
      721 gcgacccgcg taccgatccg tgaggctgag cgccatctcc agggcgttca tatcgggtgg
      781 gttgatctcc gagcgggcgt ttgcgcgctc cagtcgcctg gtctcgcgat cgacgcgaat
      841 ctcttcaggc ttggggacaa ctttcatgca gacgatgatg tgaaggtcgc gcatcgtgca
      901 cctctgattg gggatcacga gccagccgtt gagccgatct gctctgctct ccactctctg
      961 atctgttgca tgagagccgg cacaatctgg cgaaagtccc ccacgacgca atagtcggca
     1021 gactcaaaga tgggcgcttc gggatcggag ttcacggcga tgacggtacc ggccttctga
     1081 atgccgacga cgaagtggaa ggcgccgctg gcgccgcaga cgatggcgac ctgcggccgg
     1141 cagacgacgc ccgtctgccc aatttgacaa tcgcgctcga tatgaccctc gtccaccgga
     1201 gggcgggtcg cgccgacttc gccgccgatc aggccggcca gctctcgcag catatcgaag
     1261 tggccgccca cacctcgtcc gcctatcacc agggcgcgcg cctgagtcag atcggcggcc
     1321 tgccccacga cccgctccac tattcgagtg cggattatgg ccccggtcag gttcacaggt
     1381 agccgaacca ctgtgccttt ccggctgtag tcagccggtt ctggcgggaa cacgcccggc
     1441 ctgaccgtgg ccatctgggg ccggtgtttc tgcatctcaa tcagggccag cacgccgcct
     1501 ccaaaaccgg agacctcgct gacgagcacg ccggtttccg ggttcagacg cagatcggtg
     1561 caatcggcgt tcagcccggt cctcagtcga acggcgagcc ggcccgccag gtcgcggcca
     1621 ttgggcgtcg cgccaagcag gaaaatgctc ggcttgcttt ccatgatggc ctggaatgcc
     1681 acgtggccat aagcatcgac cgtgaagaca tccagcaacg gatgctcggc cagcacgatc
     1741 tcgtcggcgc cgaaggctat agcttgttcg acctgatcgg agactccatg ccccattaag
     1801 agacctgtga ggcgccaacc cacctggttg gctagctgcc tgcctttcga caggagttcc
     1861 agcgagaccc gttccaggat accgccttcc tgttcaagga agacccaaat cgcattgtgc
     1921 gcctggtcgg cgctatacag cgaggcgggc atgaaaaccc tccctcctca ttcgcgctcc
     1981 cttttttcgg tcagcgccag cgccgcaatt tcggccacat ccatgaccac cagctttcgg
     2041 atgccctgag ccttcaggct atcttccagg caggcgatac agaacgggca ggcggtgacc
     2101 aagatatcgg ccccgctggc gagagcttcg ttgacccgga tgttggaaaa ccgctcgccg
     2161 gcgtcagttt ccatccacat gcggccacca ccaccgccac agcagagcgt ctccgtgccg
     2221 gtgtgctcca tctcgaccag ctcgaggccg gggatcgcct gcaagatgcg acgcggcgct
     2281 tcgcactcat ggttgtggcg ggcgaggtag caaggatcgt ggaaggcgat cttgcgttcg
     2341 accggtcgat caaacacaag gcgtcgctgg tcgaccagtg tcgcgaggac ctgcgtgtag
     2401 tgccgcggct cgaattcctc ttcgccgcgc gggtaatgat tcttgaaagc gtcgtagcag
     2461 tggggcgaga tcgccaccag ccgggtgaca cccttctcgt ggaagacgtg cgctgcctgc
     2521 tgcgcgatct ccgcaaagta aggcttgtgc cccacgctta agacagcttc gccacaacaa
     2581 ggttcgtcgt cacccaagat gccgaaggac aggcttgccg catccaggag tcgagtcagc
     2641 gagcgggcca ccttgcgcgc ccgtccgtcg taggacgatg tgcaaccgac gtagagcagc
     2701 atctcgtgcc gatgtgcatc atacctcggg acgtgcatgc ccttcgtcca ggccgagcgc
     2761 tgcgacggcg gctgggacca ggggttgttg ttccagtaga cagaccacag caggggggga
     2821 agacccctcg gggtctggcg tcgctcccat gcgatggcgc gcaggctgcg aaacacgtcg
     2881 gtgatggcga cgcctcgcgg gcaataggct tcgcattggg cacacgtcgt gcacagccag
     2941 aggctgcttg cgcccgtgct tgccccccgc ccagcggggt ggtcgtctcc ggtgggcagc
     3001 ccgagttgtg cccggcgcag aaggtggcgc acggatacgg tttctttctt gaccagaccc
     3061 cagggacaga ttgcggtgca cacgccgcac tgatagcacg cggccgccgc gccgcccgtc
     3121 aactcgatga tctcttccca tcgtgcatcg tcaatgacga cagcctcttc atccctcatc
     3181 cgtcatccct catcgttaca acccgggcct gctgctgaac gacggcgtcc atttcatgga
     3241 tcttctcggc gaacgcttta ccttcggcgg ccgagatcca ctgcaactga agccgttcgg
     3301 gcgtgatgcc ttgccgggtg tatttgcgat gccaatggtt gaagcgcttc agggtgtggc
     3361 gattggcgta gttgtagtga caatccgagc cggtgtccgt caggcggcag cccgtgacca
     3421 ggatggcgcc ggcgcccttc tcgaagccgc gcgcgatgaa gtcctcttca agacgcgcgg
     3481 agcacatggt tcggatgatg cgggccgagg tcgggtactg acgcttctcg atgccggcca
     3541 ggtcggcccc ggcgtaggag caccagttac aggtgaagac caggcacttc tcctccggtt
     3601 tctcggccaa tgcggcatcg atctgcgcca ggatctgctc cttggtgaag gtgggcatgt
     3661 cgatggcgtc gaagttgcat tcggcggcac acgtcccgca gcccatgcag gcggcttcga
     3721 cgatgcgcac acgaccttcc ttcacaggcc cggtctgctc gatggcgctg aacgggcaca
     3781 ctttcacgca gcgcatgcag ccgatgcaca gatccaggtt gactcgggcc gtcaagggcg
     3841 ctttctcgat ctcgccacgg gacaacaagg cgccagcttt gcccgccgcc gccatggcct
     3901 gcgcgatcga ctctcgaacg tcttttgggc cctgcgcggc cccggccagg aaaacgccct
     3961 gcgtggccgt ctcagccggc cccagcttgg gatgcagttc catgaggaag ccatccgcgc
     4021 tgtgagccag tttcaactga tcggacaggc catcttcggc tggcgccagc cccacgacca
     4081 gcacgaggag atcggtgggg atgcgcaggt tggtccccag aaggtggtcg tagaagcaca
     4141 cctgccctcc cactctaact ccccactcta actcgttccc ctcaggattc aggaagcgga
     4201 tggcgtcctg cggcgggcga tcgccgtcgt agcggaagaa ccggacgccg gcccgcatcg
     4261 cctgctcgta cagttcctcg gcgtggcggc tgtaggtgcg aatgtctttg ctgatcacgt
     4321 gcaccttctt gccaagttgt cgcagccgca gggcctggtt gatcatcgag gtgcagcagt
     4381 atcgggagca gcccctgtca ccctgccggg aacccacgca ggaaacgaaa gtgattcgtt
     4441 cggcctggac ccgacacttc gccatcaggt tttccagttc gaggttggtt atgacacgcg
     4501 gatcgtcacc gcagccgaac tcgcgtggtt ggtaaggtgc cgcgcccgtg gcgactatga
     4561 tggcaccgat ccggatctcc gcgccatcgc tcaaactggc gtggaaattg cccaccacgc
     4621 cgccgacgct ctctacgatc gtgcccgagt gaatgtgcac accggcttga gcgagttctc
     4681 gcgatttggc tgcaacgaga tcacgtgcat tcaggcctgc cccttcccct tccccttcac
     4741 tccgttcagg gtcagggtca gggcaagctc tgagcgcagc cgaagggccg cccggcgcaa
     4801 tccgatcgag ctgggtgagc aaacctccta gatgctcatt gcgttccact agatccgtct
     4861 cgaatccctg gcgggccagg gtcgctgcgg ctgtcatgcc tgcaatgccg cccccgatca
     4921 ccaggacgct ctgcgtcaac ggtagatgac cgatctgcag cggcgcaagc cgccgcgcct
     4981 tttccaccgc catcgagacc atgtccatcg ccttcgtggt cgcagcagct ttatcgaact
     5041 tgtgcaccca cgaatccatg ttgcggatgt tcgccatctc caggaggtaa ggattcaatc
     5101 cggcccggat cagcacgccc cggaagatgg actcgtgggt cttcggcgag caggccgcca
     5161 ccacgacccg gttgatgctc tctcgcttaa tggcctcttc gatctcgctc tgtgtgttgc
     5221 cggcgcaaga gaacatctga tcggcggcat acaccacgtc ggggaatgtc ctggcgaatc
     5281 ccaccacctg cttcacgtcc accacgccgg cgatgttgct gccacagtgg cacacgaaca
     5341 cgcccacgcg tgatgtgcca atgtctgcca ttggttcgac cctttgccca ggctccccga
     5401 ctgaagcgtg gagcgtggag cgtggagccg ggcaggcctc gggcggctcg ggccaggatc
     5461 ggtgcgtcag gtggcttaac gcgatggccg ccgcgccgct gccttcggat acactgtcgg
     5521 ggatgtcttt cggcccgctg gcacaaccgg cggcgtagat gccgggccgg ctggtgagga
     5581 tcgggctgcc cccagtatcg gcggctcgga taaacccatc ctcgtccaca tcgatgccca
     5641 ggcgttcggc gagcctggcc agcccatccg ccggggtgac cgctgtcgcc agcaccacca
     5701 tgtcatactc ggcttcgatc cgggtcgccg actcggtatc ctcgtacacg acccgtatgc
     5761 gatccccgac cggttgtatg cgcccgggcc ggccacggat gaactttgct cccgattcac
     5821 gcgtgcggga ccagaagtcg tcgaagccct tcccgtaggc tcggatgtcc atgtagagga
     5881 cggtcacttc ggggatgccg tggtcgatcg cctgataggc atgtttgatg gaatacatgc
     5941 agcagaagcg agagcagtat cgaaagaagc gccggtcgcg taagcctacg cataggacga
     6001 cgaggatgct gtcgacgtgt ttcccatccg aaggccgcag gatctcgccg ccggtcggcc
     6061 cggctgaact gatcaggcgc tcgaactcta acgcggtcaa gatgtcggcg tgcgtcccat
     6121 agccgtattc tttcaacttt cgaggatcga gggtgtcata cccgattgcc acgatgatcg
     6181 acccgacctg tcgtgtcagt aagcatcccc tcggcatcat gaagtcgatc gccttcggcg
     6241 ggcagacctc gtcacaacgg ttgcacggca agtagttcgg cggatcgttc aaacagtgct
     6301 caatgtcgat cacgtacgcc ccgggaaccg actgtgggat cggcgtgtag atggctttcc
     6361 gaacggccat cccggcatcg tactcgttgg gcaacacgac ggggcaggct gccgtgcact
     6421 cgccgcagcg cgtgcatccg tccgtcacgt agcgccctct ctggcgaatg cggacgcaga
     6481 agtcgcccgg ccctccctcg atgctttcca cttcggccag tgataagacc tcgatgttgg
     6541 gatgcaatcc cacttccgac attttgggtg cttcgatgca gatggagcag tccagcgtgg
     6601 ggaagttctt gtcgaggacg gccatgatcc cgccgatggt cgcctcccgc tccaccagca
     6661 cgacgcgcgc tccggcttcg gccaggtcca gcgatgcctg aatgccggcg atgcctccgc
     6721 caatgacgag gaccgaatca cccatgacac ttgttccctt cgacttcgct cagggcaggc
     6781 ctggcgtgct caaaaccgac ctcgaaggcc tggaggttca gcggcgctgt ctgggccttc
     6841 accgacgact cgatcgcctc tctcatggct tcgcgatcga cgaggcccgt cacacccgtg
     6901 aagaatccca acatgaccat gttggccatg atccgccccc caagcgtctc ggccatgcct
     6961 gtggccggaa tgcggtgagg gtggtcgtcc gggagaggga tgaccaggtc ttcatcgatc
     7021 agcacaagcg catcgaatgg ggctgctggc cggaatcggg tgtaggcttc ctgcgacagc
     7081 gccacgagaa tgtcaggatg ctcgacgaag ggatagtcga cagcctcatc cgagaccacg
     7141 atgtctgcgc tcgacgcgcc gcctcgcgct tcaggaccgt aggcctgggt catgacggct
     7201 tcaagcccgg catgcaacga cagggccttg cccaggatgg tgcctgccag gacgacgcct
     7261 tgtcctccaa accccgatat ccgaatttcc ttcctcatgc cgctggctcc agccggaccg
     7321 gcttgtaggg tggctgatcg cgatcgatga agttgccgat gtaaataggc cgctcctcgc
     7381 gcaggtcgat ccccaattgc tccatcggca cgccgtcggc cagcacgcat cggctgcggt
     7441 acaactccat ttcgtctacg ccctcgttga tgttgttcga cttgccgaat cccaccggac
     7501 agggcgagag aatttcgatg aaggcaaagc ccggcttgtc gaaggcgtac agaatgtcgt
     7561 tcttaacctg ccggacatgg agcgtcgtcc agcgagagac gtggttggcg ccggcggctg
     7621 ccagcaggta gggcaggttg aacggcaact ccggattacc atagggcgag gtcgtcgttc
     7681 gggcatggaa aggcgtcgtc ggacccaact gtccccccgt catgccatag ttgaagttgt
     7741 tgatgcagat gaccttgatg tctacgttcc gccgtgcggc gtggatcaga tgattcccgc
     7801 cgatagcggc caggtctcca tcgccactga acacgctcac catcaaatcg ggacgggcga
     7861 gtttcaggcc ggtggcaaag ggaatggccc gtccgtgagt cgagtggtac gaatcgatgt
     7921 tcatgtaccc ggcggcacgg ccggagcagc cgatgcccga cactacgacg tgccgatcga
     7981 ccgggatgcc ggattccaga atggcccgag cgtaacagcg catcgcgatg ccaatgccgc
     8041 agcccgggca ccagatgtgc ggcatgcgct cggagcggat gatgtcgtcc agcggatgtc
     8101 tgttagcgac taacgctttg cccacaggcc gccacctcct ggattgcatc gagaatcggt
     8161 tcaggcggga tcatcgcgcc gccggcgtga aagacacctc gtaccggctg tttaacgtgg
     8221 cgctccacct cgcggatcat ctggcccagg ttcatttcgg cgacgatgaa cgagtcggcc
     8281 tgaccggcga gctccttgac tgtctgttca gggaaaggcc acagggatac cagccgcagg
     8341 agacctacgg ggatgccctt cccgcgggct tcgcgcacgg ctcgacgcgt ggatcgggcc
     8401 gtgcagccat aggagacgac cacaatccga gcgtcatcca actggtagcc ttccgtgagg
     8461 atgagctgat cggcgctgcg gcggaccttt tcggccaggc gggttaccag tcggtgatga
     8521 gtttcggccg acatcgcggg atagccccgc tcgtcgtggg tcagcccggt gaagtgcaca
     8581 cgataccctt cgccggcatg agccatgggc ggcaccagag ccagcccctt cgacaggctc
     8641 agggcaagct ctgagcctgt cgaagggtcg gaatcctcgg cccggaagga gtcgaagtgc
     8701 ccgttcggcg tgcctcctgg tcgcttgcgc tcccagcgtg gaatctggtc ctccggcggg
     8761 atcacgacgc gctcgaccat gtggccgatg acttcgtcgg acatcaggag caccgggacg
     8821 cggtagcgat cggccacgtt gaaggacaag atcgtcaggt cgaacatctc ttgaggcgac
     8881 cagggcgaat aagcgacgat ctcgtagtcg ccgtgcgagc caaaacgcgc ctgcatcacg
     8941 tcggactgcc cggccatcgt gggaaggccg gtcgaaggcg atcctcgctg aacatcgacc
     9001 aggacgcacg ggacctccat catgacggcg aggcccaggt tctccatcat caggctgaac
     9061 cccggcccgg aagtggcggt catcgcacgc gtgcccgcgg ctgaggcgcc gatgacggcg
     9121 gccagactgc ccagttcgtc ttccatttga atgaagaccc cgctgacctc gggcaaccgg
     9181 cgcgccagac gctccgcgat ttcggtggaa ggggtgatcg ggtagccggc gaagaaacgg
     9241 catccggccg cgatcgcgcc ctcggcgcag gcgtggtcgc catgcatgaa gtgcttgccg
     9301 gtcagcacac cgtgtgcctt cattgcgcca cctcgagcgt atagatagcg aattctggac
     9361 acaccatcgc gcagaactcg caatggacac acgattcctc ttttccgggc gtgacttcag
     9421 ggtagtgata acccttgccg ttcgccgtct ccgattcctg aaggacctgc tgcgggcaga
     9481 actggatgca gagcttacac cccttgcacc gttcgggaat gaggtagacc tgaccgcgcg
     9541 ggattcgggt ctggtgcagg ttgaggggcg tgcgcccata agctttcatg gttttgcccc
     9601 gagatgatgg tgtacgactt gtcagggcgt tgcatctgta ggttaagtga agtttgtcgc
     9661 caagtcatgt ggcctttgtc acggccacga ggttaataat tcatcctttt cacggcggta
     9721 ttgcggattg agcttgaggc gaaatcgact cacgctgcgg cgcaccaccc tcggcgtggt
     9781 ttaataagac ctcggtcatc gaaatggtct gaattgaaac gtggcgtcct tcgcattgtg
     9841 gcggtctaat ttcctgccgg atttgggaga gtcggaaacg gcgacgcggc ccatagcggc
     9901 gattatccgc tcgcgacgct cgaaacctgg gcgcggcgaa cggcctgatc tgccaactga
     9961 cctgtcccgt gtgtgatgag tgtcactcga cttccccttg caggtatcta caatgatcga
    10021 aggggtgtta tgtgttgaat gtgtcgttcc gcgctcgcgg ctgcttgcgc ccgtgcttgc
    10081 cccccgccca gcggggttgt gctgccgcca gctcgctggc cgcatagggt acaggcggga
    10141 ggcatgaccg ccggagggat ggccatgaat attcttgctg aaggtcaggg cgaactgatc
    10201 ggctggcacg atcccgacga gaatcgggag tggatcctgg agaacaaacc gcgcgacctg
    10261 cgcgataagc gcacgagcgt gagcgacgcg gtcacgagac tcgtccagga cggtgacttc
    10321 atcgccttcg gcggtttcgg tcacattcgg attccgatgg cgctggtgta cgagatcatc
    10381 cgccagcgca ggcgcaatct cgtcatggcc gggaagacgg ccgtccacga tgtggatctt
    10441 ctgatcggcg ccgggtgcgt gaatcggatc gaggtagcgt atgcttttgg ccacgaactg
    10501 cgcggtctgt cgccggcagg ccgccgcgcg gtcgaaaccg ggcagtgccg cgtggtggcc
    10561 gagatctcca acgccggcta ccagtggcgc tttctagcgg gcatgatggg cattcccttc
    10621 atcccgtcgc gcaacatgtt aggcacggat accttcaagc acagcgcgtg caaggtcgcc
    10681 accgatccgt ggagtggaaa accggtctgc ctgatcccgg cggcataccc cgacgttgcc
    10741 atgtttcacg tgccgcgctg cgacatgtac ggcaacgccc agatcgacgg cattatcgtg
    10801 gaggactttg agctggcccg cgcggcccga cgcgtgatca tcactgcgga ggaagtcgtc
    10861 gacgaagcga tcattcggaa ccagccatgc cgcacggtga tcccgtttta tgtggtcgat
    10921 gccgtgtgcg aggtgccgca cggcgcgcac cccacgctga tgccctacct gtacttcttc
    10981 gacgaagagc acattggcga gtggctcgag ctgtcgcaga cgcctgaggg ggcagccgcc
    11041 tactttgaca agtatgtctt tggcgtgtgc tgcttcgagg aatacctgga gcgtatcggc
    11101 ggcatccgga agatgcacta cttaaagcag gtggagcaat tgaaggcgcc gttgcgcgcc
    11161 ccttggttga agacgaggaa aggataagcc gtcatggctg cacaggtgtt ggagtacacc
    11221 cagaccgaac tgctcagcgc tgttgcggcg cacctgctgg aggacaagaa gtccgtgctg
    11281 gtgggcacgg gcctgccgat gatcgccgcg ctgctcgcgc agcgaatgca cgcgccgggg
    11341 ctgctcatca ttttcgaggc gggagggatc ggcccgcgcg ttccgacgct gccgatctcg
    11401 gtgggcgatt cacgcacttt ctatcgcgcg gcagcggctt ccagcatgca cgacgtgatg
    11461 agcgcctgcc aggccggcta cgtagactac gggttcctgg gcgcggcgat gatcgaccag
    11521 tatggcaaca tcaacacgac cgtcatcggc gactgggaac agccggcggt gcgcctgccc
    11581 ggcagcggcg gcgcgaacga catcggctca ctgtgccatc gcaccatcat catcctgcgc
    11641 caggacgagc gcaaatttgt cgagcgcgtg aacttcatca cgacgccggg ctacctggat
    11701 ggccccggcg caagggaacg ggcgggcttg ccgtatggca gcggccctta ccgcgtgatt
    11761 acccagttag gcgtctatgg cttcgacgac gagacgaaga gattgacact cctggccatg
    11821 catccgggtg tcacacggga cgacgtacga gccaatagca gttgcgaagt cctcattccc
    11881 cgggtggtcg atacgactgc gccaccgacc gaagaagagc agcgattgct ccgcgagatc
    11941 gacccggcgg gcatggtgat tggaaggtag gtgcgccgcg ctgcattcca atcccgctgg
    12001 ggcgagcgcg cgttttcgat cgcctttcgc tcgtgcgcga ttccgggcct gacgctcgtc
    12061 gcgatgggga tcgtccatct tcaacttgaa tccccgaacg tggccggtgc tgtggcggtt
    12121 cctggtgacc ggcaagtgag cggcgcggga ggttcccgtg agcagaccc
//
LOCUS       DBNHXB010000222         3483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1057071, whole genome shotgun sequence.
ACCESSION   DBNHXB010000222 DBNHXB010000000
VERSION     DBNHXB010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1057071"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1586
                     /gene="malQ"
                     /locus_tag="AC162G_09195"
     CDS             78..1586
                     /gene="malQ"
                     /locus_tag="AC162G_09195"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009632384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR5076030.1"
                     /translation="MRFPRSSGILLHPTSLPGRYGIGDLGDDAYRFVDFLADAKQSLW
                     QVLPLGPTGYGDSPYQCFSAFAGNPLLISPDKLLEEGLLAEDDVRSVPPFPSGAVDFG
                     EVIDYKRGLLNRAARRFRAGANDRLREAFDTFCAEQAYWLEDFTLFMALKDAHDGAMW
                     NAWPRDIATRKPDALKPQADKLASTVFAHKFLQFLFFRQWSAVKRYANERGIRIIGDI
                     PIFVAYDSADVWSHPDLFFLDEQGSPTVVAGVPPDMFTETGQLWGNPLYRWDVMAERR
                     YAWWIDRFRAALEQVDIVRLDHFIGFTRYWEVPAGEKTAENGRWVPGPGAALFHAVER
                     ALGKLPIIAEDLGVVTPQVEALRDQFEFPGMKTIQFAFTGPDNPFLPHNYTQNYVVYT
                     GTHDNDTSRGWFSTASPEEKSFAQRYLARNGEDFAWDLIRTAMSSVADTVVVPLQDVL
                     DLGSEARMNFPGRPAGNWKWRFTWDRLDDEHRDRLREMVELYGREPMKKDES"
     gene            complement(1613..1876)
                     /locus_tag="AC162G_09200"
     CDS             complement(1613..1876)
                     /locus_tag="AC162G_09200"
                     /inference="COORDINATES: protein motif:HMM:NF052803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7990 family protein"
                     /protein_id="HIR5076031.1"
                     /translation="MSTSLRRFNRLFAEVLYGMAVHDQVRALTRQRGSLEHLFVLISF
                     GDLLGVPILPPYYGLRLLPFVVPLINNWRRRMLRERDLIDSMF"
     gene            complement(1934..2914)
                     /locus_tag="AC162G_09205"
     CDS             complement(1934..2914)
                     /locus_tag="AC162G_09205"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014433.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HIR5076032.1"
                     /translation="MPQIETSMRDYFRSHPNLKYVFFGGKGGVGKTVSAGVTAQWLAG
                     QGKRTLLASTNPVHSLSGLLGQDVLGKHTPVAGVSNLWAYEIDTKDTIERSKVEIREK
                     IQWFLKFADISTKADAFVESATMNPAFEESAMFENMIDLMFKAEYDAYVFDTAPTANA
                     RRLLGMSKVYSLWVNKMLKSREEARTLREMLSFTKKKEKDPLMEYLIAFRDRMEHARV
                     LLTDPQQTAFFFITLPEALPIAVITRFIGWFHEFGIPVGGVIVNMLIDKSQVKPDSPD
                     FVRNRVAMQDRYMDDIWQKFDGMVRATVPLYETEVRGVQSLARMGGAVFV"
     gene            complement(2940..3089)
                     /locus_tag="AC162G_09210"
     CDS             complement(2940..3089)
                     /locus_tag="AC162G_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013522.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HIR5076033.1"
                     /translation="MPLVRISLRTGKSADYRRAIGDAVHRAMVELVFRQWGSAVCVIG
                     GIWRE"
     gene            complement(3161..>3483)
                     /locus_tag="AC162G_09215"
     CDS             complement(3161..>3483)
                     /locus_tag="AC162G_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HIR5076034.1"
                     /translation="FFVVVPEEMIILDTQKAAKLFAAFDVPIAGYVVNRVVPHELLDQ
                     DIPAYLRNRIEMQDRYLGRIKTDLGDQIRAYVPELERDVTGLPMIERLAGIMYGDGYP
                     RSAS"
BASE COUNT          681 a         1073 c         1077 g          652 t
ORIGIN      
        1 gagagagtcg ggcttcgtgt ggtgaccatt gtgaggcgta agaggtaaca agcgatacgc
       61 tataggcgat aagcgatatg cgatttccac gttctagcgg cattctgctg catccgacat
      121 cgctgcccgg ccgatacggc attggcgatc tgggagacga tgcctatcgc ttcgtcgatt
      181 ttctggccga cgcgaagcaa agcctgtggc aggtgctgcc gctcggcccg accggttacg
      241 gcgactcccc gtaccaatgc ttttcagcct tcgcagggaa ccccctattg atcagccccg
      301 acaagctgct cgaagagggc ctgttggccg aagacgacgt gcgctcggtc ccgccgttcc
      361 catccggcgc cgtcgacttc ggcgaggtca tcgactacaa gcgtggcctg ttgaaccggg
      421 cggcgcggcg attcagggcc ggagccaacg acaggttaag agaggcgttc gacacgttct
      481 gcgccgagca ggcatactgg ttggaggatt tcacgctgtt catggcgctc aaagacgcgc
      541 acgacggcgc gatgtggaac gcctggccgc gcgatatcgc cactcggaag ccggacgctc
      601 tgaagccgca ggccgataag ctggccagca ccgtgttcgc ccacaagttc cttcagttcc
      661 tgttctttcg ccagtggagc gccgtcaagc gctacgcgaa cgaacgcggc atacgcatca
      721 tcggcgatat tcccattttc gtggcgtacg acagcgccga tgtatggtcg caccccgatc
      781 tattcttcct cgacgagcag ggcagtccga ccgtcgtggc cggcgtgccg cccgacatgt
      841 tcaccgaaac gggccagttg tggggtaacc cgctgtaccg ctgggacgtg atggccgagc
      901 gccgatatgc ctggtggatc gatcgcttcc gcgccgcgct ggagcaggtc gacattgtgc
      961 gcctcgacca cttcatcggc ttcacgcgct actgggaggt tccagcgggc gagaaaacgg
     1021 ccgaaaacgg gcgctgggtg cccgggccgg gagcggcgct gttccacgct gtcgagcgcg
     1081 cactgggcaa gctccctatc atcgcggagg atttgggagt cgtcacgccg caagtggagg
     1141 cgctgcgtga ccaattcgag ttccccggta tgaagaccat ccagttcgca ttcaccggcc
     1201 ccgacaaccc cttcctgcca cataactaca cccagaacta cgtcgtgtac acgggcacgc
     1261 acgataacga caccagccga ggctggttca gcaccgcttc acccgaagag aagtccttcg
     1321 cacagcgcta cctggcgcgc aacggcgagg actttgcctg ggacctcatc cgcacggcga
     1381 tgagctccgt cgccgacacg gtcgtcgtgc cgctgcaaga cgtgctcgat ctgggcagcg
     1441 aggcgcgcat gaatttcccc ggccggccgg ccggcaactg gaaatggcgc ttcacgtggg
     1501 ataggctcga cgacgagcac cgggatcggc tgcgcgagat ggtggagctg tacgggcggg
     1561 agccaatgaa gaaggatgaa agttgacttc ttcatgagtc attcgtcgct catcaaaaca
     1621 tcgagtcgat gagatcgcgc tcgcgcagca tgcgccgccg ccagttattg atcagaggaa
     1681 cgacgaaggg cagcagacgg aggccgtagt acgggggcaa gatcggcacg cccagcaggt
     1741 cgccgaagct gatcaacacg aagaggtgct ccaggctgcc gcgctgccgg gtgagcgccc
     1801 gcacctgatc gtggacggcc atgccgtaga gaacttcggc gaagagacga ttgaagcggc
     1861 gaagagaggt ggacatcgtt gaaacgtgat gcgtgatggc gtgagtcgag ggcgcgttat
     1921 cacgcctgac ccatcacacg aacacagctc cacccatacg ggccagcgac tgcacgccgc
     1981 gcacctcggt ctcgtacagc ggcacggtcg cgcgcaccat gccgtcgaac ttctgccaga
     2041 tgtcgtccat gtagcgatcc tgcatggcga cgcggttgcg gacgaagtcg ggcgagtcgg
     2101 gcttgacttg cgacttgtcg atcagcatat tcacgatcac gccgccgacc gggatgccga
     2161 actcgtggaa ccagccgatg aagcgggtga tgacggcaat cggcagcgcc tccggcagcg
     2221 tgatgaagaa gaaggccgtc tgctgcggat cggtcaacag cacgcgggca tgctccattc
     2281 gatcgcggaa agcgatcagg tactccatca gcgggtcttt ctctttcttc ttcgtgaacg
     2341 agagcatctc gcgcagtgtg cgggcctctt cgcggctctt cagcatcttg ttaacccaca
     2401 gcgaatagac cttcgacatg cccagcagcc gccgcgcgtt cgccgtcggc gcggtgtcga
     2461 agacgtaggc gtcgtactcg gctttgaaca tcaggtcgat catgttttcg aacatggccg
     2521 actcttcaaa cgccgggttc atggtggccg attcgacgaa cgcgtccgcc ttcgtcgaaa
     2581 tgtcggcgaa cttgaggaac cactgaatct tctcgcggat ctctaccttg ctgcgctcga
     2641 tggtatcctt cgtgtcgatc tcgtaagccc acaagtttga aacgccggcg acgggcgtgt
     2701 gcttgccgag aacgtcctgc cccagcagac ccgacaggct gtgcaccggg ttcgtcgaag
     2761 ctagcagcgt gcgcttgccc tgcccggcga gccactgcgc ggtgacgccg gcggagaccg
     2821 tcttgcccac gccgcctttg ccgccaaaga agacgtattt gagattcggg tgcgaccgga
     2881 agtagtcgcg catggaagtt tcaatttgcg gcatcttttc tccttttagc gcctgtgact
     2941 tactctcgcc agataccacc gatcacgcat actgcgcttc cccattgccg aaagaccaac
     3001 tcgaccattg cgcggtgaac agcgtcgccg atcgcccggc gatagtcagc agatttgcct
     3061 gtgcgaagcg agatacgaac gagcggcatg ggagactccg cgtgaagcgt ccaccctcag
     3121 gggttgagaa aaaccgggcg cacaaatgct gccagatcgc tcatgaggct gaccttggat
     3181 atccgtcccc atacatgatc ccggccagcc tctcgatcat cggcaggccg gtcacgtcgc
     3241 gctccagctc cggcacgtag gcccggatct ggtcgcccag atcggtctta atgcggccga
     3301 ggtatctgtc ctgcatttcg atccggttcc tgagatacgc cggaatgtcc tggtcgagca
     3361 gctcgtgcgg caccacgcgg ttcaccacgt agccggcgat cggcacgtcg aaggcggcga
     3421 agagcttggc cgccttctgc gtgtcgagga tgatcatctc ctcgggcacg acgacgaaga
     3481 aga
//
LOCUS       DBNHXB010000223         8590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2903529, whole genome shotgun sequence.
ACCESSION   DBNHXB010000223 DBNHXB010000000
VERSION     DBNHXB010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8590
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2903529"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1230)
                     /gene="coxB"
                     /locus_tag="AC162G_09220"
     CDS             complement(175..1230)
                     /gene="coxB"
                     /locus_tag="AC162G_09220"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR5076035.1"
                     /translation="MRAFNKTRTRSIILVSSLVALLLALSLPTIALAEPDGPPSALEP
                     ASPVSAAIADLHTVVLIIAAGVFVIVEGLILFAAFRFRRRAKDAGEPPQIHGNTRLEI
                     AWTVAPAVIVVALFVLAVRAQQNMDAAAYSAEPGVPISVEVIGHQWWWEFRYPDLGVA
                     TAGQMVIPVGRAIHLKITSADVIHSFWVPELSGKTDAIQGVINQSLLKTSRPGDYYGQ
                     CAELCGVSHAYMRLVITAVSEGEFAAWATSQAKDAVEPTEEAAQAGQQVFQSAGCVGC
                     HVIRGVPGAVGKTGPDLTHVYGRPCFAGCTLATTPYNLAKWLADPPAVKAGSLMPNLN
                     LPPADIESLVAYLQTLK"
     gene            complement(1784..4228)
                     /locus_tag="AC162G_09225"
     CDS             complement(1784..4228)
                     /locus_tag="AC162G_09225"
                     /inference="COORDINATES: protein motif:HMM:NF040138.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR5076036.1"
                     /translation="MRSKLSVIPVVVSVVLSLWAPVSGASENFAESQQGQVGPRPVVA
                     YATRSDTTVALRRMPQIPPRPAVLGEIFETRPPKLLPGRQGSSGPSGPDPVLQGPVPG
                     VSAPTTGANFEGVNNVNGVLPPDTVGDIGPNHYVQMVNLSFAIWDRNGNKLYGPVDNN
                     TLWQGFGGPCAATNDGDPIVLYDHLADRWMMSQFALPSFPRGPFYQCIAVSQTGDPLG
                     AWHRYEFKISDSKLNDYPKFGVWPDGYYMGVNQFTCNFVSCNWAGQGVVVFERDQMLN
                     GQPARMVYFDLFNVDSNLGGMLPSDLDGSPPPSGAPNVFSQVDDNAWGYSPDQLQLWE
                     LRVNWTNPASSTFTHAASLGTAAFDSNMCGYSRNCIPQPGGTNVDAISDRLMYRLQYR
                     NFGSHQTLVVNHTVDVNGSNRAGIRWYELRNTGGGWGLYQTGTYSPDSHHRWMASMAM
                     NGAGDMALGVSVSSSSVYPSIRATGRLDGDSLGQMTQGEVEIATGSGYQTHSAGRWGD
                     YSMMAVDPVDDCTFWYTQEYYAVVGSAPWQTRVGSFKLRDCGPANNPPTVSIVNPAGG
                     STVVGTVTVQIGATDTEDAAGTLTVEWNVDGGAWQPATYNSISGYYEASWNTTGVGDG
                     SHMLNARATDSGGKTGTDSNAVTVDNVNDPPVASFTYSCGGLTCNFDASGSSDPDGTI
                     TSYAWDFGDGTAGSGTTASHTYAAAGTYNVTLTVTDDDGATDAHTQGVMVSDGAITLT
                     ANGYKVRGLQKADLTWSGATSPNVDIYRNNALIATTENDGFYTDNINNRGGGSYTYKV
                     CEAGTSICSNEATVTF"
     gene            complement(4530..4784)
                     /locus_tag="AC162G_09230"
     CDS             complement(4530..4784)
                     /locus_tag="AC162G_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HIR5076037.1"
                     /translation="MDERVFTEAELRQYDGTKGRPVYIACNGIVYDVSDAPLWRTGMH
                     QELHYAGLDLTRSLRKAPHDGRVFERKYVRAVGRLRSEDS"
     gene            complement(4822..5154)
                     /locus_tag="AC162G_09235"
     CDS             complement(4822..5154)
                     /locus_tag="AC162G_09235"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5076038.1"
                     /translation="MKGPQMFKDYRDHVGARADKHFKTTLFQGDHVMVGLNCLEPGQT
                     QEAHAHAAADKFYYVIEGRGAFTIGDEVREAGPGMMVWAPAGASHGVENRSETRLTLL
                     VGIAPPPR"
     gene            complement(5236..7068)
                     /locus_tag="AC162G_09240"
     CDS             complement(5236..7068)
                     /locus_tag="AC162G_09240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR5076039.1"
                     /translation="MAAKSNGQGALPHWDLSNVYPGLESEEFSVAVAETKSTLGDLEA
                     YLAERQIAKLPPDSPAARDAAAVKATIDGYLDRMNAIQRQYATLRAYVWSFVTTDSYN
                     TTAKRLLSELEMQGVRLQQAGVRFQAWIGSVEGALPGVLEQDGPAKVHAFYLRELAEQ
                     SRYLMSDAEESLAAELALSGPSAWSRLQGTVCSQLTVDFERDGKVEKMPIPALQNLSH
                     DPDPGVRRRAYEAELAAWETVREPLAAALNGVKGATTTLNKRRGRVDALHASIDQARI
                     DRETLETMIGAMQDSFPMFRRYLRAKAKRLRHEGGLPWWDIFAPVGKSERRYTWPETR
                     EFILTHFATFSDRLAGLARRAFDNNWIDAEPRDGKRGGAFCMSVDAVDESRVLCNFDG
                     SLDQVGTVAHELGHAFHNECHVGKTFLQTVTPMTLAETASIFCQTIITDAALANVSGP
                     EEELAILETFLIDAAQVIVDITSRYLFEKEIFERRAKSELSADDFCEIMLRGQKATYG
                     DGLDERHLHKYMWAWKPHYYFAGLSFYNYPYAFGLLFGLGLYAIYKERGKSFVPEYEA
                     LLASTGEGTPADLAARFGIDIRKPDFWRGSLNLIGQRIDRYVGL"
     gene            complement(7109..8377)
                     /locus_tag="AC162G_09245"
     CDS             complement(7109..8377)
                     /locus_tag="AC162G_09245"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5076040.1"
                     /translation="MVTSRFTGLWRHADFMKLWAGQTISEFGSVVSRDALPLVAVLTL
                     TASPAQMGVLAALGSLPALLIGLPAGAWVDRTRRKPIMIAADVASAALLASIPVAYLL
                     GALRMEQLYVVAALAGGLALLFDVAYTSYLPSLVERQHVVEGNSKLSASESVSEIGGS
                     ALAGALVQTLSAPLAVAVDALSFLASAVSLALIRKPEPRPAPPPNPNLRREIADGLRA
                     LATHSILRALTAAAITGVFFGNFYAALYGLYAIRELGIGPAALGLLIASGGIGSFLGA
                     MSAAPAARRFGVGPVLIGSAVLSGVLGLLTPLAAYVGAAAVAFLFVAQIAGDLVRTVY
                     TIHALSLRQIVTPDRLLGRMNASVEFVQGGAATIGVLAGGLLGQALGVQAAVWIAALG
                     SMLPVVWLIASPVRRLPAFGGLPDGRLTIQ"
BASE COUNT         1616 a         2778 c         2695 g         1501 t
ORIGIN      
        1 caggcggatg agcagggcct ggatgccccc ggcgataaag aagaggaagg cagaccacac
       61 gtacagcagg ccgatcttct tgtgatcgac cgtggtgagc cattccatca ggctgcctcg
      121 gcggcgcggc gcaacggcgc taatcgtacc ggttatcgtg gccatgtatc atccttattt
      181 cagagtctga agatacgcga cgagcgactc gatgtccgcc ggcggcaggt tgaggttggg
      241 catcagcgag ccggccttga cggccggcgg gtcggccagc cacttcgcca ggttgtaggg
      301 cgtagttgcc agggtgcatc cggcgaagca cgggcggccg tagacatggg tgaggtccgg
      361 ccccgtcttg cccaccgcac ctggcacgcc tcgaatcaca tgacacccaa cgcagcccgc
      421 gctctggaag acttgttgac cggcctgcgc tgcctcctcg gtaggctcga cggcatcctt
      481 cgcttggctt gtcgcccacg ccgcaaactc gccttcggac accgccgtga tgaccagacg
      541 catgtaggcg tgggagacgc cgcacaactc ggcgcactgt ccgtaatagt cgcccggccg
      601 gctggtcttc agcagcgatt gattgatcac gccctgtatc gcgtcggtct tgccgctcag
      661 ctcaggcacc cagaaactgt ggatcacgtc cgccgaggtg atcttgagat ggatggcccg
      721 cccgacgggg atcaccatct gccccgccgt cgcgacaccc agatcaggat agcgaaactc
      781 ccaccaccac tgatgaccga tcacctcgac ggagatcggc acgccgggtt cggccgagta
      841 ggcggccgca tccatatttt gctgtgccct taccgccagc acgaacagcg ccacgacgat
      901 gaccgccggc gcgaccgtcc aggcgatctc gaggcgcgta ttgccgtgaa tctggggtgg
      961 ctcgccggcg tccttcgccc ggcggcggaa gcgaaaggcg gcaaacagga tcaagccctc
     1021 cacgatgaca aacacgccgg cggcgatgat gagcacgacg gtgtggagat cggcgatggc
     1081 ggcggataca ggagaagccg gttcgagggc agacggtggg ccgtcgggtt cggccaatgc
     1141 aatagtgggg agagagagtg ccaggagcag tgcaaccagg gacgagacaa gaattatact
     1201 ccgggtccgc gtcttattga acgcgcgcat cggtcctccg tgcccgtgat gtgaagccgt
     1261 caaatggtgc gtcacaaagg gaggctcttg ccagatcaaa gctgcccacc gtcgcaagag
     1321 ccataagtga atctttgcgc gattatacca taccggaagg aaaatacaaa cggaagccgc
     1381 tcactgacta ccccgtgcac gccgcacgtg gccgagtcgg acggcgccag gcgtatcatg
     1441 ccgaggaggc aggacgaggc gtctcggccc gggcacgcgg cactcgccgg tcgcacgaga
     1501 gattcctcac tacgttcgga atgacacgct gcaaaggtga cgcgccgcac atggccgagt
     1561 caggcggcac cgctcgtgtc atgccgagga ggcacgacga ggcatctcga tcccggtgcg
     1621 ccgcgctcgc cggccagacg agagattcct cactgcgttc ggaatgacac gccgcacgga
     1681 tctcccacca aagtcggcag agccaaaaac aggggatgca gcctgcctgt ggccgcatcc
     1741 cctgcttgcg ccgttaccct gcaacagatg tcatcagccg ccactaaaag gtaaccgtcg
     1801 cctcattgga acagatgcta gtgcccgcct cacacacctt ataagtatag ctacccccgc
     1861 ctctgttgtt gatgttatcg gtgtagaagc cgtcgttctc ggtggtggct atcagcgcgt
     1921 tgtttcgata gatgtccaca tttggcgagg tagcgccgct ccacgtcagg tcggccttct
     1981 gcaaccccct caccttgtac ccatttgccg ttaaggtgat cgcgccgtcg ctgaccatca
     2041 cgccctgggt gtgtgcgtcc gtagcgccat cgtcgtcggt gacggtgaga gtgacgttgt
     2101 aggtgccggc agccgcatag gtgtggctgg cagtggtgcc agaaccggcc gtcccatccc
     2161 cgaagtccca ggcgtagctg gtgatcgtcc catccggatc ggaggagcca gaggcatcga
     2221 agttgcaggt caagccgccg cacgagtaag tgaaggaagc caccggcgga tcgttgacgt
     2281 tgtccaccgt aactgcattg ctgtcggtgc ccgttttgcc tccgctgtct gtggcgcgcg
     2341 cgttgagcat atgggatccg tcgccgacgc cggtcgtgtt ccagcttgcc tcgtaatatc
     2401 cgctgatgga gttataggtc gccggctgcc aggccccgcc gtccacgttc cactcgacgg
     2461 taagtgtgcc cgctgcgtct tcggtgtcag tcgcgccgat ctggacagtc accgtgccca
     2521 cgaccgtgct gcctccggcg gggttcacga tgctcacagt cggcggattg ttcgccgggc
     2581 cgcagtcgcg cagcttgaag gagccgacgc gtgtctgcca tggagcgctg ccgactacgg
     2641 cgtagtattc ctgcgtgtac caaaaggtgc agtcatccac cgggtcaacc gccatcatgc
     2701 tgtagtcgcc ccagcgcccc gcgctatggg tctggtagcc gctgccggtt gcgatctcca
     2761 cctcgccctg ggtcatctgg cccaaggagt cgccatccag acggccggtg gcacggatcg
     2821 acggatagac cgacgagctc gacacagaga ctcccagggc catgtcaccc gcaccgttca
     2881 tcgccatgct ggccatccag cgatgatggg aatccggaga gtaagtgccc gtctggtata
     2941 ggccccagcc gccgcctgtg ttgcgcagct cgtaccagcg aatgccggcg cgattcgaac
     3001 cgttcacatc caccgtgtga ttgaccacca gcgtctggtg gctgccgaag ttgcggtatt
     3061 ggagtcggta catcaaccgg tcggagatag catccacatt cgttccccct ggctgcggga
     3121 tgcagttgcg ggagtagccg cacatgttag agtcgaaggc agccgtgccc aaggaagctg
     3181 cgtgagtgaa ggtagagcta gccgggttag tccagttgac tcgaagctcc cagagctgca
     3241 actggtcggg cgagtagccc cacgcgttgt catcgacctg gctgaagaca ttgggggcgc
     3301 ctgagggcgg cggcgacccg tcgaggtcgg atggaagcat accgcccagg ttggagtcga
     3361 cgttgaatag atcgaagtac accatccgcg ctggctgacc gttcaacatc tggtcgcgct
     3421 cgaagaccac aacgccctgg ccggcccagt tgcaggagac gaagttacac gtgaactggt
     3481 tgacccccat gtagtagcca tcgggccaca cgccgaactt gggatagtcg ttcagcttgc
     3541 tgtcgctgat cttgaactcg tagcggtgcc aggcaccgag tgggtcgccg gtctgagaga
     3601 cggcaatgca ctggtagaac ggcccgcgcg ggaagctcgg cagcgcgaac tggctcatca
     3661 tccagcggtc ggccaggtgg tcatagagga cgataggatc tccgtcgtta gtcgccgcac
     3721 acgggccgcc gaagccctgc catagcgtgt tgttgtcgac cgggccgtag agcttgttac
     3781 cattccgatc ccagatagcg aaggaaagat tgaccatctg gacgtaatga ttggggccga
     3841 tgtcgcccac ggtatcgggc gggagcacac cgttgacgtt gttgacaccc tcgaagttgg
     3901 cgccggtggt cggcgcactc acgcctggga ccgggccctg cagcactggg tcaggccccg
     3961 agggtcccga gctcccctgg cggccgggca ggagcttggg tgggcgtgtt tcaaagatct
     4021 cgcccagaac cgccggccta ggtgggatct gcggcatccg gcgcaaggca accgtggtgt
     4081 cgcttcgggt tgcatacgcg acaacggggc gcggtccgac ttgaccttgc tggctctcag
     4141 caaagttctc ggacgcgccg ctcaccggcg cccataggct cagcacaacc gacacaacaa
     4201 cgggaattac agatagtttg ctacgcatca gaacatcctc cgtatgtgat atatagagct
     4261 ggggcatacg cacttcgacg gagccagctc aagccctccg gcgagccgtt catatcaact
     4321 tcaaacgact ccaaatacaa caacgtcctc gcgcaagaag ccgcctgcgc gaggacgaag
     4381 tcccaggccc tgtgagaaga agtgccagca gaaagtcaat cccaagtctt agcccacccg
     4441 gcaggatcga acaccaccct gcccattcaa tctatcgcca ttataaagcc aaattccaga
     4501 caaatcgggt gagaacggtt agattttggt tacgagtctt cgctcctcag gcggccgacc
     4561 gcgcggacat actttcgctc gaacacgcgc ccatcgtgcg gcgctttgcg caggctgcgt
     4621 gtcaggtcta agccggcgta gtgcagttcc tggtgcatgc ctgtccgcca cagcggcgcg
     4681 tcgctcacat cgtatacgat accgttgcag gcgatgtata cgggcctgcc tttagtacca
     4741 tcgtactggc gaagttcggc ctcggtgaaa acacgctcat ccaacctcca acctccaact
     4801 tccaacctcc aatgtccagc ctcacctcgg cggcggcgcg atgccgacca ggagcgtcag
     4861 tcgggtttcg cttcgattct cgacgccgtg tgaggcgccc gccggcgccc agaccatcat
     4921 ccccggcccg gcttcgcgca cctcatcgcc aatcgtgaac gcgccccggc cttcgatgac
     4981 gtagtagaac ttgtcggcgg cggcgtgtgc gtgcgcctcc tgcgtctgcc cgggttccag
     5041 gcagttgagg ccgaccatca cgtggtcgcc ctgaaacagc gtcgtcttga agtgcttgtc
     5101 ggcccgcgcg ccgacgtggt ctcgatagtc tttgaacatc tgagggcctt tcacatatcg
     5161 catatcgcgt atcgcttagg gctgatagcc gatggctgat cgccaatagc ttatcgcatg
     5221 ccgccttcat cctttctaca agccgacata tcgatcgatg cgctggccga tcaggtttag
     5281 gctgcctcgc cagaagtccg gcttgcggat gtcgatgccg aagcgggcgg cgagatcggc
     5341 aggtgtgccc tcgccggtgc tggcgagcag cgcctcgtat tcgggcacga aggatttgcc
     5401 gcgctccttg tagatcgcgt acagccccag gccgaagagc aggccgaaag cgtaggggta
     5461 gttgtagaac gacaggccgg cgaaataata atggggtttc cacgcccaca tatacttgtg
     5521 caggtgacgc tcgtcgaggc cgtcgccata cgtcgccttc tggccgcgca gcatgatctc
     5581 gcagaagtcg tccgccgaca gctccgactt cgcgcggcgc tcgaagattt ccttctcgaa
     5641 caggtagcgc gacgtgatgt cgacgatcac ctgcgctgcg tcgatcagga acgtctccag
     5701 gatagccagt tcttcctccg ggccggatac gttggccagc gccgcatcgg tgatgatcgt
     5761 ctggcagaag atcgaggcgg tctcggccag cgtcatcggc gtaaccgtct gcagaaaagt
     5821 cttgcccacg tggcattcgt tgtggaaagc atggcccagc tcgtgcgcca cggtcccgac
     5881 ctgatcgagc gagccgtcga agttgcacag gacgcgcgac tcgtcgacgg cgtcaacaga
     5941 catacagaac gcgccgcccc gcttgccgtc gcgcggctcg gcgtcgatcc agttgttgtc
     6001 gaaagcccgc cgcgccaggc cggccagccg atcgctgaac gtggcgaagt gcgtcaggat
     6061 gaactctcgc gtctccggcc aggtgtaacg ccgctcactc ttgcccaccg gcgcgaagat
     6121 gtcccaccac ggcagcccac cttcgtgtct gagccgttta gccttagccc tcaagtatcg
     6181 ccggaacatg gggaacgagt cctgcatcgc gccgatcatc gtctccagcg tctcgcggtc
     6241 gatgcgtgcc tgatcgatcg acgcgtgcag ggcgtcgacg cgcccgcggc gcttgttcag
     6301 cgtggtagtc gcgcccttca cgccgttgag cgcggcggcc agcggctcac ggacagtctc
     6361 ccaggcagcc agctcggcct cgtaggcgcg gcggcgcacg ccgggatcag gatcgtggct
     6421 caggttttgc agcgccggta tcggcatctt ttcgactttg ccatcgcgct cgaaatcgac
     6481 ggtgagctgc gagcagacgg tgccctgcag tctgctccag gcactcggcc cgctcagcgc
     6541 caactcggcg gccagcgatt cctcggcgtc gctcatcagg tagcggctct gctcggccag
     6601 ctcgcgcagg taaaaggcgt ggaccttcgc cgggccgtcc tgttcgagca cgccgggaag
     6661 cgcgccttcg acgctgccaa tccatgcctg aaagcgaacg cctgcctgct gcaggcgcac
     6721 gccctgcatc tcaagctcgg acaacaggcg cttcgccgtc gtgttgtatg agtccgtcgt
     6781 cacgaacgac cagacgtaag cgcgcagcgt cgcgtactgc cgctggatgg cgttcatgcg
     6841 atccagatag ccgtcgatcg tcgccttcac ggcggcagca tccctggctg ccggtgagtc
     6901 cggcggcagc ttcgcgatct gccgctcggc caggtaggct tcgagatcgc ccagcgtgga
     6961 tttcgtctcg gcgacggcca ccgagaattc ttccgactcg aggccgggat agacgttcga
     7021 taagtcccag tgtggcaggg cgccctgccc gtttgacttg gcagccatgt tgtctccaaa
     7081 ggtgaaattg tgcggtctcc cgcaggcgct attgtatcgt caagcggcca tctggcaagc
     7141 cgccgaaggc aggcaacctg cgaaccggcg aagcgatcag ccacacgacc ggcagcatgg
     7201 atcccagcgc cgcgatccac acggccgctt gcacaccgag cgcctgaccc agcaggccgc
     7261 cggccagcac gccgatcgtc gccgcgccgc cctgcacgaa ctcgacgctg gcgttcatcc
     7321 ggcccagcag ccgatcgggc gtgacgatct ggcgcaggct cagcgcgtga atcgtgtaga
     7381 ccgtgcgcac caggtcaccc gcgatttgcg cgacgaacag gaacgcgacg gcggccgcgc
     7441 ccacatacgc ggccagcggt gtcagcagac cgagcacgcc gctcaacaca gccgagccga
     7501 tcagcactgg cccgacgccg aaacgccggg cggccggcgc ggccgacatc gcgccgagaa
     7561 acgatccgat tcccccgctc gcgatcaaca gacccagggc agccgggccg atgcccagct
     7621 cgcgaatcgc gtacagcccg tacagcgcgg cgtagaagtt gccgaagaag acgccggtga
     7681 tcgcggccgc agtcagcgcg cgcaagatcg aatgcgtcgc cagcgcgcgc agcccgtcgg
     7741 cgatctcgcg tcgcaggttc ggattcggcg gcggcgcggg gcgtggctcc ggctttcgaa
     7801 tcagcgccag cgaaaccgct gacgccagaa aggacagtgc gtcgacggcg accgccagcg
     7861 gcgcgctgag cgtctgcacc agcgcgcccg ccagcgccga cccgccgatc tcggagaccg
     7921 actcgctcgc gctgagcttg ctattgcctt ccacgacatg ttggcgctcg acgagcgacg
     7981 gcaggtagga ggtgtacgct acgtcgaaca gcagcgccaa ccccccggcc agcgccgcga
     8041 cgacgtacag ttgctccatg cgcagagcgc ccagcagata ggcaaccgga atggaggcca
     8101 gcagcgccgc gctggccaca tcggcggcga tcatgatcgg cttgcgtcgc gtccggtcga
     8161 cccaggcgcc cgccggcagc ccgatcagca aggccggcag cgatccgagc gcggccagca
     8221 cacccatctg cgcgggcgag gctgtcagcg tcagcacggc gacgagcggc agcgcatcgc
     8281 ggctgacgac cgagccgaac tcggagatcg tctgaccggc ccacagcttc atgaagtcgg
     8341 cgtggcgcca caggccggtg aagcgcgaag tcaccatcag tagatccgga aacgctctga
     8401 gaggattgcc aaatccccgg tttggcctgg atttggcaat ccaggccgag caaaatcgga
     8461 tctacggtat ctgtagcgca ggcgcacaac acaaggcccc gaccgcgcat cggggccaag
     8521 tgtgtgagga gggagggcag ccaaagccgc cctcccagtg ctggacgaga aggagtggct
     8581 aagggccacc
//
LOCUS       DBNHXB010000224         3838 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1225598, whole genome shotgun sequence.
ACCESSION   DBNHXB010000224 DBNHXB010000000
VERSION     DBNHXB010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1225598"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..614
                     /locus_tag="AC162G_09250"
     CDS             72..614
                     /locus_tag="AC162G_09250"
                     /inference="COORDINATES: protein motif:HMM:NF016064.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HIR5076041.1"
                     /translation="MNWLITQLRKPEWSPYVAGAGLGLVTILALVLSSALLPAPVLIG
                     ASGAYENLVAPAGLAVDPKNTYFNYIMPPGLTWSVWILAGVFLGGLASALLSRTFKWR
                     ALPDRQWTEIFGKSVVKRWAIVFLAAVLLEYAAGIAGGCTSGLAISGGVVLAPASFIF
                     IAGMFASGIVTAMLLYRKKY"
     gene            691..1272
                     /locus_tag="AC162G_09255"
     CDS             691..1272
                     /locus_tag="AC162G_09255"
                     /inference="COORDINATES: protein motif:HMM:NF016064.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HIR5076042.1"
                     /translation="MTLIAPILIGFLFGFLLHKGGMTRYHKIVNVFRFTDMAVLKFMM
                     SSLVVGMIGIYLLTDLGVVASMPITPTYVVGNLVGGLLFGVGMAAAGVCAAPIAAGSG
                     EGRLDYIFPGVFGMLAGALLFGLTYNQVYPAISKIANEGPIVLPEVIDANHWLVIGLF
                     AAITIVLFYTLERAIRLRRNRVASEQQSVISNQ"
     gene            1404..3653
                     /locus_tag="AC162G_09260"
     CDS             1404..3653
                     /locus_tag="AC162G_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5076043.1"
                     /translation="MADQIIQLTRRSFLKASGATLGALGLAGMGFGAASAEPAADGPL
                     TYDKIVPTMCEMCVWRCGVWAKVRDGRAVKLEGNPYHTHSRGRLCARGQSGIMTAYDP
                     DRVQFPLIRAGKRGEGRFRRASWDEALDFTAKQMLEIKEKYGPEAMIFSATHNLSQPL
                     FENLLYAYGSPNYGTQRSLCFNAMVVAHLMTYGIEEPGRDYRTAKYVIYTGRNMLEAI
                     STSETSDLVEAIARGAHVVVLDPRFTKTASKASEWLPIKPGADLAFHLALLNVIVGEN
                     RYHHEFVALNTAGFDELKAEVAKYTPEWAAPITGVDAGTIRRIARDFAAAGPDAMAHP
                     GWRTSNFVNSFQTERAVAILNALVGNWGRTLVNNGGEEGGIQLGKPPQPPYPRITAQR
                     LDGVPWKYPLVPLKLGVFQEIRDNILSGKPYQARGWFIARQNPALSLPERHKTLEAFG
                     KLDFIVTLGIIPNDTEWYADVILPEASYLERYDPLNVVENRVFLRRPVIEPIGESKSA
                     LWIYKQLGERLGLGDYFQYEDEVDYINQQLAPLGIDIGAFAERGCWSPTLEAISAAER
                     SSCFVPLAITFEEEELPYELKEFKFSTPSGKIELASETLRNAGQPAVPAWEAPPEPEP
                     SAFYLLTGKVGQHTQFATQNNLWLNQVYAENELWIHPKPAAGRGIVNGDVVKVTSDAG
                     TVTIKAKVTEGIREDCVWMTQGFGHRSMGLKTAYGKGASDSDVHVTITDPVSGGQALS
                     QTYVTVEKA"
     gene            3736..>3838
                     /locus_tag="AC162G_09265"
     CDS             3736..>3838
                     /locus_tag="AC162G_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024207.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR5076044.1"
                     /translation="MANNVKKYGFVIDPRRCIDCRACLVACRAENDVP"
BASE COUNT          754 a         1202 c         1190 g          692 t
ORIGIN      
        1 tgaatcgtaa agcgtgaatt acgttttacg tttcacgcac caagcaacac taattaacgg
       61 gagaatatgc tatgaactgg ctcatcactc aactgcgtaa gcccgaatgg tcgccctacg
      121 tggccggagc cggactcggt ctcgtcacga tcctcgcgct ggtgctcagc agcgccctct
      181 tgccggcgcc ggttctcatc ggcgcgtcgg gcgcgtacga gaacctggtc gcgccggcgg
      241 ggcttgccgt cgatccgaag aacacctact tcaactacat catgccaccg ggtctcacct
      301 ggtccgtgtg gatactggcc ggtgtcttcc tcggcgggct ggcttcggcg ctgctgtcgc
      361 gcaccttcaa gtggcgcgcc ctgcccgatc gacagtggac ggaaatcttc ggcaagagcg
      421 tcgtcaagcg ctgggcgatc gtattcctcg ccgccgtgct gctggagtac gcagcaggca
      481 ttgcaggcgg ctgcaccagc gggctggcga tctccggcgg cgtcgtgctg gcgcccgcct
      541 cgttcatctt catcgccggt atgttcgcgt cgggcatcgt cacggcgatg ctgctgtatc
      601 ggaagaagta ttgaggtgat gcgtgatcac atgatcacgt gatcacgtaa tgtcgtcgcc
      661 accgaaagac actttaagga acatactcct atgacactca tcgctcctat cttgatcggc
      721 tttctgttcg ggtttctgct gcacaaaggc ggcatgaccc gctatcacaa gatcgtcaac
      781 gtgttccgct tcaccgacat ggcggtgctc aagttcatga tgtcttcgct ggtggttggg
      841 atgatcggca tctatttgct gaccgacctg ggcgtcgtcg ccagcatgcc catcacgccg
      901 acctacgtcg tcggcaacct ggtcggcggc ctcctgttcg gcgtgggcat ggcggcggcc
      961 ggggtatgcg ccgcgccgat cgccgccgga tcgggcgagg gccgcctgga ctacatcttt
     1021 cctggagtct tcgggatgct ggccggcgcg ctgctcttcg gcctgaccta caatcaggtc
     1081 taccccgcga tcagcaagat cgccaatgaa gggccgattg tgctgcccga agtcatcgac
     1141 gccaatcact ggctggtgat cggcttgttt gcggcgatca cgatcgtgct gttctataca
     1201 ctcgagcgcg cgatccgatt acggcgcaac agagtagcca gtgagcagca atcagtaatc
     1261 agtaatcagt aatcagtgac cagtgaacag tgaacagcag ccagtagcca gtgagcagcg
     1321 acgcaccgat taccgatcac cgatcaccga tcactgacca ctgaccactg actactgact
     1381 actggtcact tccagaggtg gttatggcgg accaaatcat ccaactcacc cggcgcagct
     1441 ttctgaaggc ctcgggcgcg acgctcggcg cattggggtt ggccggcatg ggcttcggcg
     1501 cggcgtcggc agagcctgcc gcagacggac cgctgaccta cgacaagatc gtgccgacga
     1561 tgtgcgagat gtgcgtgtgg cgctgcggcg tatgggccaa agtgcgtgac ggacgcgccg
     1621 tcaagctcga aggcaacccc taccataccc attcgagggg gcgtctgtgc gcgcgcggcc
     1681 agagcggcat tatgaccgcc tacgatccgg accgcgtgca atttccgctg attcgcgccg
     1741 gcaagcgcgg cgagggcagg tttcgccgcg cgtcgtggga cgaagcgctc gatttcaccg
     1801 cgaagcagat gctggagatc aaggagaagt acgggccgga ggcgatgatt ttctccgcga
     1861 cccacaacct gagccagcca ctgttcgaaa acttgctgta cgcttatggc tcacccaact
     1921 acggcacgca gcgcagcctg tgcttcaacg cgatggtcgt tgcacacctg atgacctacg
     1981 gcattgaaga acccgggcgc gactatcgca ctgcgaagta cgtcatctac accggccgca
     2041 acatgctcga agcgatctcg accagcgaga cgagcgacct ggtcgaggcg atcgcgcgtg
     2101 gcgcgcacgt cgtcgtgctc gatccgcgct tcaccaagac ggcgagcaag gcgagcgagt
     2161 ggctgccgat caagccgggc gccgatctcg ccttccacct ggcgctgctc aacgtgatcg
     2221 tcggcgagaa tcgctaccat cacgaattcg tcgcgctcaa caccgccggc ttcgacgagc
     2281 tgaaagcgga agttgccaag tacacgccgg aatgggccgc gccgatcacc ggcgtcgacg
     2341 cgggaacgat ccgccgcatc gcgcgcgact tcgccgccgc cggcccggac gcgatggcgc
     2401 atcccggctg gcgcaccagc aacttcgtca actccttcca gaccgagcgc gccgtggcga
     2461 ttctcaacgc gctggttggc aactgggggc gcacgctggt caacaacggc ggcgaagagg
     2521 gcggcattca gttgggcaag ccgccgcagc cgccctaccc gcgcatcacc gcccaacggc
     2581 tcgacggcgt gccgtggaaa tatccgctgg tcccgctcaa gctcggcgtg ttccaggaga
     2641 ttcgcgacaa catcctgagc ggcaagccgt atcaggcgcg cggctggttc atcgcgcggc
     2701 agaacccggc gctcagcctg cccgagcgac acaagacgct cgaagccttc ggcaagctcg
     2761 atttcatcgt caccctcggc atcatcccga acgataccga gtggtacgcc gacgtgatcc
     2821 tgcccgaggc atcgtacctg gagcgctacg atccgctgaa tgtcgtcgag aatcgcgtgt
     2881 tcctgcgccg gccggtgatc gagccgatcg gcgagagcaa gagcgcgctg tggatctaca
     2941 agcagctcgg cgagagactg gggcttggcg actacttcca gtacgaggac gaggtcgact
     3001 acatcaacca gcaactggcc cccctgggca tcgacatcgg cgcgttcgcc gagcgcggct
     3061 gctggagccc gacgcttgaa gcgatcagtg ccgccgagcg atcgtcgtgt ttcgtgccgc
     3121 tggcgatcac gttcgaggaa gaagagctgc cgtacgaatt gaaagagttc aaattcagca
     3181 cgccgagcgg caagatcgag ctcgcttcgg aaacactgcg caacgccggg cagccggccg
     3241 tcccggcgtg ggaagctccg cccgagccgg aaccaagcgc gttctacctg ctcaccggca
     3301 aggtcggcca gcacacgcag ttcgccactc agaacaacct gtggctcaac caggtgtacg
     3361 cggaaaacga gttgtggatt caccccaagc cggctgccgg gcgcggcatt gtcaacggcg
     3421 acgtggtgaa agtcaccagc gatgccggga ccgtgacgat taaggccaaa gtcaccgaag
     3481 gcatccgcga agactgcgtg tggatgacgc agggtttcgg gcatcgatcg atgggcctga
     3541 agaccgcgta tggcaaaggg gcgagcgaca gcgatgtaca cgtcaccatc accgatccgg
     3601 tcagcggcgg gcaggcgctg tcgcagacgt acgtgacggt ggagaaagca tagggcatat
     3661 cgcgtatcgc ctatcgccta tcgcagagct ttccgcaata agcgatacgc cataggcgat
     3721 aagcggagat agagcatggc gaataacgtg aagaagtacg gctttgtcat cgatccccgg
     3781 cgctgtatcg actgccgcgc ctgtttggtc gcgtgccgcg ccgagaacga cgtgccgc
//
LOCUS       DBNHXB010000225        11306 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2903581, whole genome shotgun sequence.
ACCESSION   DBNHXB010000225 DBNHXB010000000
VERSION     DBNHXB010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11306)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11306)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11306
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2903581"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1819
                     /locus_tag="AC162G_09270"
     CDS             <1..1819
                     /locus_tag="AC162G_09270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5076045.1"
                     /translation="NVFAWGGGFHLSPGGKSLVFMWNKTGRWQLYSLPIAGGEARQLT
                     YAEESVVSPRWSPDGKWIAYLQDYAGDENFDLFVLDPATGETRNLTPDTPDEAINWAV
                     RWMPDQSGFVYVSNRDGHFATYLLPAESSVPKRLTHYEFSDTFAQPSPDGRWIAVETM
                     AADGKMGVFLVPLAGGEPIPIGDAASAIDASGARWSPDSSKLAFTSDAKDSCDVGIFD
                     IATRTIEWLTGGAHECYYPVWSPDGKRLAYAENREGNLGLTIHALDGTIERHADMPGI
                     HSQIEWTPDGRSLVFTYSGPDYPADLWLFSPFDPARRGVPSAPLRGSASSSGPARQLT
                     DSLPRTIDRSVFVRPTHVTYPSLDEGVRVPALLYTPHGAKRGGSNPAVLFVHGGPTAQ
                     HDNDFLAAVQDLAMRGYVVLAPNYRGSTGYGRQWREANRFDIGRADTNDVAAGADYLA
                     REGWAEPRRIGITGVSHGGYLTMTSLTFHPEKFAAGSALVPFVNWFTGHENSRGDLQY
                     WDLQHMGDPVKDGKRWRAMSPIFFVDRIAAPVQLFAGAHDPRCPLDESEQIRDALRQR
                     GRPVELHVYADEGHGFRKIENRVDAYKKRATFFDRYLRR"
     gene            1926..2858
                     /locus_tag="AC162G_09275"
     CDS             1926..2858
                     /locus_tag="AC162G_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020518192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076046.1"
                     /translation="MPEPVIVTENLTKRYGSIIAVRNLNLSVPRGAVFGFLGPNRAGK
                     TTTIRLLLGLVRPTAGRARVMGHDVVTARQMVLPQVGALVESPTFYPFLSGRDNLRVL
                     AHTGGYPDEARVDEVLDITGLADRQRDRVRTYSLGMKQRLAVAATLLNRPQLLILDEP
                     TNGLDPAGQAEVRALIRRLGQAGHTVFISSHLLHEIEQVCDRVAIIDKGELLREGKVS
                     ELLGDLPTLRIEAEPIERAYAIVAGLDGLAVERDGTNQIVVRAGRERAPEIVDALSSG
                     RTRVYQVIVERETLEQVFLRITGAHREDDMEKRK"
     gene            2855..3682
                     /locus_tag="AC162G_09280"
     CDS             2855..3682
                     /locus_tag="AC162G_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076047.1"
                     /translation="MTAGLLRAEWIKLRRMRITWLVLLLPAAAGLCGAILPTLTAADA
                     VRRFGTRILQSLDTFAFPQSMLFGLQIVELLGSILIIVFITSAVGNEYGFDTWKNLLA
                     RHGRRGAFLLAKLLYALAAVTALIVIVPILVHLSMLVTFRAATGFDFPLQAAPGAWQT
                     FGGAWLLAWLRLAITASIGVFATVITRSGGAGVAIAVSWLLVDALANSLGAAFETSAW
                     RTIAPFTFNFSLNALAAYLRGDAGDVTLLHSLANLAVYTVGLTVLAMLLFRRRDIAG"
     gene            3694..5067
                     /locus_tag="AC162G_09285"
     CDS             3694..5067
                     /locus_tag="AC162G_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR5076048.1"
                     /translation="MILGPTFEEMLHPDTIDAETRAEAVAAMTRDPLDPINLFNITWR
                     DAGNRIRYEVLPRQLTGVEANIAVLYGKDFPSGSHKVGAAYSVLVDLETAGEVDPSVH
                     TLVWPSTGNYGIGGAWVGCRMGFKSIVVLPEGMSAERFERIEGYGASIIKTPGSESNV
                     KEIYDKTWELRRSDPNVRILNQFEVMGNYRFHYHVTGNTIVELASKLKVQGVGDGTIA
                     AFCSAMGSAGTIAAGDRLKQVWPHHKIVGLEPIQCPTLYNNGYGSHDIQGIGDKHVTW
                     IHNVMNMDAIMCLDDVECKKGLQLLTDPIGMGTLEKRFDVPREKVEKLSTIFGISGVC
                     NVLGAIKTAKHYGFGAKDTIVTICTDAIDRYYSVMADMTGPYGKMDEAEAVARAHIFL
                     DQKTDWIKDGTPDMRRQWHHLKYYTWVEQQGKSVEELNAQLSEDWWLAEQAKVAQIDA
                     RIKQARA"
     gene            5093..5773
                     /locus_tag="AC162G_09290"
     CDS             5093..5773
                     /locus_tag="AC162G_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076049.1"
                     /translation="MQPDSSILVAVMNSPRDFEIARDEGWYRIPQRSAPKFFPPEFVA
                     FYFTKPFEQDAYSVRWYAKVRGHELASRRQLFPGEADHPRADHVYYKLQLGPLVELPH
                     PIPSKRWRRLTFILTTGGRLFGAWEINDLVVGSREHDLMWRALKEAGLHAERDDELPS
                     RKRVDFLLPCSYGDLGIVVSDEAPAAGEVLMHFTPKQINESLDDCIYQIQSAVTERGG
                     LTPPSSQP"
     gene            5853..7004
                     /locus_tag="AC162G_09295"
     CDS             5853..7004
                     /locus_tag="AC162G_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5076050.1"
                     /translation="MNALDGIRVIDLTEALAGPYCTMILGDLGADVVKVERPGVGDQA
                     RKWGPPFVGGESAYFLAVNRNKRSAAIDITAGDGQRAMRGLLERADVFVCNVRRVESL
                     RKYGLDPETTRRFNPRLVYCSITGYGRTGPYAGRGGYDLVAQGEAGLMAATGEPDGEP
                     MRWPVAIADMTTGVHSAIAILAALLSRERSGRGQFIDSALLDSQLSWAPIMASGYFAT
                     GQRPPRLGNVHATIAPYQVFKARDKHMIVAVGTDRLWASFCEVIGLGPEVRDDPRFVS
                     NPDRLVHRGELIPILETIFVQHDAAHWLERLRAAEIPCGPINALDETLSDPHMLARGM
                     VFVMEHPVGMIKMLGSPLHLSDTPVTYRRPPPLLGEHTDEVLQEAMSWS"
     gene            7175..7558
                     /locus_tag="AC162G_09300"
     CDS             7175..7558
                     /locus_tag="AC162G_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076051.1"
                     /translation="MREMMGPLMVDQTIRQAIQHCWLSLPDDERSPDRVEQEILRVVH
                     RALQNMREDAAAFGFSKEEKSSWEERLARMRAQYPKAYEKWTEEDDSLLLQKFGEGML
                     MADLARLFQRQPSAIRSHLAKLGLI"
     gene            7655..8329
                     /locus_tag="AC162G_09305"
     CDS             7655..8329
                     /locus_tag="AC162G_09305"
                     /inference="COORDINATES: protein motif:HMM:NF017984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HIR5076052.1"
                     /translation="MARFDIPRQRLRNQGIAGATFKKPGDVVEWLAAVQAQDYAGAKW
                     ALGLRLQDATDDDVEQAFAEGAILRTHLLRPTWHFVTPADIRWMLVLTAPRAHAVNAD
                     MSRKLELDNALFKRSNAALARALHGGRQLTRDELRGVLQKAGIATDGGLRLGYLLMRA
                     ELDGMVCSGARRGKQFTYALLDERAPHAKTLGREEALAELAQRYFVSRGPATVQDFAK
                     RSFTHA"
     gene            8322..9218
                     /locus_tag="AC162G_09310"
     CDS             8322..9218
                     /locus_tag="AC162G_09310"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013731363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR5076053.1"
                     /translation="MPNRPIVILDAPSNLGLRPPEQGAVPGCYKLPWALRDRKLVSAI
                     DATDAGSLVPPRYRAAWNPGDGDRNADAIAAFSTMLADRVGSILTPNGLAFVLGGDCS
                     ILIGNTLALKRRGRYGLVFLDAHSDFRHPQNAPAIGAAAGEDLAIVTGRGDPRLINLE
                     NLGPYIRDEDVMLIGVRDDDECLPELAELGIHVITSARLEEVGVQQAATHALATAAQA
                     TQGFWVHLDLDIVDATEMPAVDCPEPNGLSFSALTALLKSLLESPSCVGLELTIYDPD
                     LDPTGYYADQVMACISAVFRSS"
     gene            9328..10329
                     /locus_tag="AC162G_09315"
     CDS             9328..10329
                     /locus_tag="AC162G_09315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5076054.1"
                     /translation="MTHDQITVANAPAISGLSFRHFRGESDYPHMVAVIMSSADADKI
                     ERADTVDDIANNYNHLTNCDPYKDMIFAEIAGEVIGYSRGWWWDEPATRRMYGFVGFL
                     VPAWRRKGIGRVMLLWMENHLRGIATTHPIDRARFFQVSVSQFQAGTSIMLERAGYRP
                     IRYFHEMARPSLNDIPDVPLPDGLEMRPALPEHYRLIWASVDETSQDEWGYTGEDYEA
                     WLNNMPHFQPHFWQIAWDSATERIAGHVLTFIDHAENERFNRKRGYTEGIGVGRAWRR
                     RGLARALIARSLQAQKAEGMSESALVADSDSISGVTRLYESCGFRVVRRNAIYRKPF"
     gene            complement(10413..10637)
                     /locus_tag="AC162G_09320"
     CDS             complement(10413..10637)
                     /locus_tag="AC162G_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002748708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HIR5076055.1"
                     /translation="MPTLPMVRGAEVVRVLQKAGFQIVRQRGSHVRLHHADGRVVTVP
                     VHAQQDVGRGLLRKILRDADLSPEEFVELL"
     gene            complement(10637..10870)
                     /locus_tag="AC162G_09325"
     CDS             complement(10637..10870)
                     /locus_tag="AC162G_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015201823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR5076056.1"
                     /translation="MAGDIERGKQEFYVLIERDEDGTFVGEVPQLRGCYSQGKTLDEL
                     MKNIQEVIELCLEEQAESGEAIPEFVGIQKVTV"
     gene            complement(10947..>11306)
                     /locus_tag="AC162G_09330"
     CDS             complement(10947..>11306)
                     /locus_tag="AC162G_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076057.1"
                     /translation="SIARAALANPRILILDEATSSVDTRTERLIQQALEGLLSGRTSF
                     VIAHRLSTVKHADQILVLRDGQIVERGKFRELLDARGFFYDLYMSQFRRQEPAGGNGR
                     VSGDGRQSQAVTVAGAA"
BASE COUNT         2157 a         3559 c         3511 g         2079 t
ORIGIN      
        1 caacgttttc gcctggggcg ggggatttca cctgtctccc ggcggcaagt ccctcgtctt
       61 catgtggaac aagaccgggc gctggcagct ctacagcctg ccgatcgcgg gcggcgaggc
      121 cagacaactc acgtacgccg aagaatcggt cgtctcgccg cgctggtcgc ccgacggcaa
      181 atggatcgcc tatttgcagg attacgccgg cgacgaaaac ttcgacctgt tcgtgctcga
      241 tcccgcgacg ggggagacgc gcaacctgac tcccgacacg ccggacgaag cgatcaactg
      301 ggcggtgagg tggatgcccg accaatccgg cttcgtctac gtctcgaacc gcgacggcca
      361 tttcgcaacg tacttgctgc ccgccgagag cagcgtgccg aaacggctga cccactacga
      421 gttcagcgac acattcgcac agccttcgcc cgatgggcgg tggatcgccg tcgagacgat
      481 ggccgccgac ggcaaaatgg gcgtcttcct ggttccgctc gcgggtggcg aaccgatccc
      541 gatcggtgac gcagcgagcg ccatagacgc atccggcgcg cgctggtcgc cggacagctc
      601 gaagctggcc ttcacatcgg atgcgaagga cagttgcgac gtcggcatct tcgacatcgc
      661 cactcgcacg attgaatggc tcactggcgg cgcgcacgag tgttactatc cagtctggtc
      721 gccggacggg aagcggctgg catatgccga gaatcgggaa gggaatctcg ggctgacgat
      781 tcacgcgctg gatggaacga tcgagcgtca tgccgacatg cctggcattc attcgcagat
      841 cgagtggacg ccggacggcc gctcgttggt gttcacgtat tcgggccccg actacccggc
      901 agacctgtgg ctgttttcac ctttcgaccc cgctcggcgg ggcgtcccct ccgctccgct
      961 acgaggatct gcgtcaagca gcggtccggc tcgccagctt accgactcgc tgcctcgaac
     1021 aatcgaccgg agcgtcttcg tcaggcccac tcacgtgacc tacccgagcc tcgacgaggg
     1081 agtccgcgtg cctgcgctcc tgtacacgcc gcacggcgcg aagcgcggcg gctcgaaccc
     1141 ggccgtgttg ttcgtccacg gcggcccgac ggcgcagcac gacaacgatt tcctggcggc
     1201 cgtccaggac ctggcgatgc gcggctatgt cgtcctcgcg ccgaactatc gcggcagcac
     1261 gggctacggt cggcagtggc gcgaagccaa tcgcttcgac atcggccgcg ccgacacgaa
     1321 cgacgtcgcg gcaggcgccg attaccttgc ccgcgagggc tgggccgagc cgagacgcat
     1381 cgggatcacg ggcgtgtcgc acggcggcta cctgaccatg accagcctca cctttcatcc
     1441 agagaagttc gcggccgggt cggcgctcgt gcccttcgtc aactggttca cgggacacga
     1501 gaactcgcgc ggcgacctgc agtactggga cttgcagcac atgggcgatc cggtgaagga
     1561 tggcaagcgc tggcgcgcga tgtcgccgat attcttcgtc gaccggatcg ccgcgcccgt
     1621 gcagctcttc gccggcgcgc acgatccgcg ctgtcccctc gacgagtcgg agcaaatccg
     1681 cgacgcgctt cggcagcgcg gtaggcccgt cgagctgcac gtgtacgcgg acgaaggcca
     1741 cggcttccgc aagatcgaga accgggtcga cgcatacaag aagcgtgcga cgtttttcga
     1801 ccgatacctg agacgataga ctgcagacct ggcaggtctt gtccgcgcag gttagctgta
     1861 cactagttcg caaagacctg ccaggtctac ccagagaatt caagtgcgtt tgggatgacc
     1921 tcgccgtgcc tgagcctgtc attgtcaccg agaacctcac gaagcgctac ggctcgatca
     1981 tcgccgtgcg gaatctcaac ttgagcgtgc cgcgcggggc ggtgttcgga ttcctcggcc
     2041 cgaaccgggc cggcaagacg acgacgatcc gcctgctgct cggcctggta cgcccgacgg
     2101 cgggccgcgc gcgggtgatg ggccatgacg tcgtcacggc gcgccaaatg gtcctgccgc
     2161 aggtcggcgc gctggtcgag tcgccgacgt tctacccgtt tctgtccgga cgcgacaatc
     2221 ttcgcgtgtt ggcacacacg ggcggctatc cggacgaagc gcgcgtcgac gaagtgcttg
     2281 acatcaccgg ccttgccgat cgccagcgag atcgcgtgcg gacgtattcg ctcggcatga
     2341 agcagcgact cgccgtcgcc gcgacgctcc tcaaccgccc tcaactgctg atcctcgacg
     2401 agccgacgaa cggtctcgat ccggccgggc aggccgaggt acgcgcgctc attcggcggc
     2461 tgggccaggc gggccacacc gtcttcatct ccagccatct ccttcacgag atcgagcagg
     2521 tgtgcgaccg cgtggcgatc atcgacaagg gggagctgct gcgcgaaggg aaagtctcgg
     2581 aactgctcgg cgatctgccc acgctgcgca ttgaggctga gccgatcgag cgggcgtacg
     2641 cgatcgtggc cggcctggac gggctggctg tcgagcgcga cggcacgaat cagatcgtcg
     2701 tcagagccgg gcgcgagcgc gcgccggaga tcgtcgacgc gctgagcagc ggccggacgc
     2761 gcgtttacca agtcatcgtc gaacgcgaga cgctggaaca ggtcttcttg cgcatcaccg
     2821 gcgcgcatcg cgaggacgac atggagaaaa ggaagtgaca gccgggctgc tgcgcgccga
     2881 gtggatcaag ctgcgccgga tgcgcatcac ctggctcgtg ctgctcctgc cggccgcggc
     2941 gggactatgt ggagcgatcc tgcccacgct caccgccgcg gacgccgtgc gtcgattcgg
     3001 aacccgcatc ctgcagtcgc tcgacacatt cgcgttcccc cagtcgatgc tattcgggct
     3061 gcaaatcgtc gagcttctcg gcagcatcct gatcatcgtc ttcatcacct cagccgtcgg
     3121 caacgaatac gggttcgaca cgtggaagaa cctgctggcg cgccacgggc ggcgcggcgc
     3181 gttcctgctc gccaagctcc tgtacgcgct ggccgccgta acggccctga tcgtcatcgt
     3241 gccgatcctc gtgcacctga gcatgctcgt gacttttcgg gcggcaaccg gcttcgactt
     3301 cccgttacag gccgcgccgg gcgcctggca gacatttggc ggcgcatggc tgctggcgtg
     3361 gctgcggctc gctatcacgg cttcgatcgg cgtgttcgcc acggtcatta cgcgatccgg
     3421 cggcgccggc gtcgccatcg ccgtgtcgtg gctgctcgtc gacgcgctgg cgaacagcct
     3481 gggcgcggcg ttcgagacaa gcgcctggcg caccatcgcg ccgttcacgt tcaacttcag
     3541 cctgaacgcg ctggcggctt acctgcgcgg agatgccggc gatgtcacgc tgctccacag
     3601 cctggccaac ctggctgttt acacagttgg cctgaccgtt ctggcaatgc tgctatttcg
     3661 ccgccgcgat attgcaggat agtggagaaa ccaatgattc tcggaccgac tttcgaagag
     3721 atgctccacc ccgacacaat cgacgccgaa acgcgcgccg aggcggtcgc cgcgatgacg
     3781 cgcgacccgc tcgatccgat caacctgttc aacatcacct ggcgcgacgc tggcaaccgc
     3841 atccgctacg aagtgctgcc caggcagttg accggcgtgg aagcgaacat cgccgtcctg
     3901 tacggcaagg atttcccatc cggctcgcac aaggtcggcg cggcgtactc ggtgctggtc
     3961 gatctcgaaa cggccggcga ggtcgatccg agcgtccaca cgctcgtgtg gccgtcgacc
     4021 ggcaactacg gcatcggcgg cgcgtgggtc ggctgccgca tgggattcaa gagcatcgtc
     4081 gttctgccgg agggcatgag cgccgagcgc ttcgagcgca tcgaaggcta cggcgcgtcg
     4141 atcatcaaga cccccggcag cgagagcaac gtcaaagaga tttacgacaa gacctgggag
     4201 ctgcgccgct cggatccgaa cgtccgcatc ctcaaccagt tcgaagtgat ggggaactac
     4261 cgctttcact accacgtcac cggcaacacg atcgtcgagc tcgcgtcgaa attgaaggtg
     4321 cagggcgtcg gcgacgggac gatcgccgcg ttctgctccg cgatggggtc ggcgggcacc
     4381 atcgcggcgg gcgaccggct caagcaagtc tggccgcatc acaaaatcgt cggcctggag
     4441 ccgatccaat gccccacgct gtacaacaac ggctacggct cgcacgacat ccagggcatc
     4501 ggcgacaagc acgtgacgtg gattcacaac gtgatgaaca tggacgcgat catgtgcctg
     4561 gacgacgttg agtgcaagaa gggactgcaa ctgctgaccg acccgatcgg catgggcacg
     4621 ctggagaagc gtttcgacgt cccgcgggag aaggtcgaga agttgtcgac gatcttcggc
     4681 atcagcggcg tgtgcaacgt tctgggcgcg atcaagacgg ctaagcatta cggtttcggg
     4741 gcgaaagaca cgatcgtcac catctgcacg gacgccatcg accgttacta ctcggtgatg
     4801 gccgacatga ccgggccgta cgggaagatg gatgaagcgg aagccgtggc gcgtgcgcac
     4861 atcttcctcg accagaagac agattggatc aaggacggca cgccggacat gcgccgccag
     4921 tggcaccatc tcaagtatta cacctgggtc gagcagcagg gcaagagcgt cgaggagctt
     4981 aacgcgcaat tgagcgaaga ctggtggctg gccgagcagg ccaaggtcgc ccagatcgac
     5041 gcccgcataa agcaggcaag ggcatgatga aatctattct cgtagggtca ctatgcagcc
     5101 cgattcgtcc atcctcgtcg ccgtgatgaa ctcgccgcgc gatttcgaga tcgcgcggga
     5161 cgaaggctgg tatcgcatcc cgcagcgatc ggcgccgaag ttcttcccgc cggaattcgt
     5221 cgcgttctac ttcacgaagc cgttcgagca ggacgcgtac agcgtgcgct ggtacgcgaa
     5281 ggtgcgcggg cacgagctgg cctcgcggcg gcagttgttt cccggcgagg ccgatcatcc
     5341 gcgcgccgat cacgtctatt acaagctcca gctcggcccg ctcgtcgagc ttccccaccc
     5401 tatcccgtcg aagcgctggc ggcggctgac gttcatcctg acgacgggcg ggcgactgtt
     5461 cggcgcgtgg gagatcaacg acctggtcgt cggctcgcgc gagcacgacc tgatgtggcg
     5521 cgcgctgaaa gaggccgggc ttcacgccga gcgcgacgac gagctgccct cgcgcaagcg
     5581 cgtcgacttc ctgctgccgt gcagctacgg cgacctgggc atcgtcgtat cagacgaggc
     5641 gccggctgcg ggcgaggtct tgatgcactt cacgccgaag cagattaacg aatcgctcga
     5701 cgactgcatc tatcagatcc aatccgccgt caccgagcgc ggcggcctga ctccaccctc
     5761 cagtcagcca tgagccgcgt gcgccaagct attcaaaggc taattgacca cctgactacg
     5821 tgactacgtg actatatgac caggagacca ccttgaatgc cctggatggc atccgcgtca
     5881 tcgatctaac cgaggcgctc gccgggccat actgcacgat gatcctgggc gacctgggcg
     5941 ctgacgtcgt caaagtcgag cgtcccggcg tcggcgacca ggcgcgcaag tggggcccgc
     6001 cgttcgtcgg cggcgagagc gcctacttcc tcgccgtcaa ccgcaacaag cgcagcgcgg
     6061 cgatcgacat cacggccgga gacggccagc gcgcgatgcg cgggctgctc gagcgcgccg
     6121 acgtgttcgt gtgcaacgtg cgccgcgtcg aatcgctcag gaagtacggc ctcgacccgg
     6181 agactacccg ccgcttcaac ccgcgcctgg tgtactgctc gatcaccggc tacgggcgga
     6241 cgggtcccta cgccggccgg ggcggatacg acctcgtcgc gcagggcgag gcggggctga
     6301 tggccgccac aggcgagccg gacggcgagc cgatgcgttg gccggtcgcc atcgccgaca
     6361 tgacgaccgg cgtacattcg gcgatcgcca tcctggcggc gctgctcagc cgcgagcgga
     6421 gcgggcgcgg ccagttcatc gacagcgcgc tgctcgactc gcagttgtcg tgggcgccga
     6481 tcatggcctc gggttatttc gcgaccgggc agcgaccgcc gcgcctcggc aacgtgcacg
     6541 cgactatcgc gccctaccag gtcttcaagg cgcgcgacaa gcacatgatt gtcgccgtcg
     6601 ggacggaccg cttatgggcg agcttctgcg aagtgatcgg cctcggtccc gaagtgcgcg
     6661 acgatccacg attcgtgtcc aaccccgatc ggctcgtgca ccggggcgag ctgattccca
     6721 tcctggaaac gatcttcgtt cagcacgatg cggcgcactg gctcgagcgg ctgcgcgcag
     6781 ccgagatacc gtgcggcccg atcaatgcgc tcgacgagac gctgagcgat ccgcacatgt
     6841 tggcgcgcgg catggtgttc gtcatggagc atccggtcgg gatgatcaag atgctcggca
     6901 gcccgctgca cctgtccgac acgccggtca catatcggcg gccgccaccg ctgctgggtg
     6961 agcacacgga cgaggtattg caagaggcca tgtcttggtc gtaacagcca agtcgacctc
     7021 gcgtgcgctc gacgccgccg cccgggcgac cgtctcccgg aacgatcact gggccgctac
     7081 aacgtgagtg aggtctcaaa tgactgatga taaaccacct gtctctcaaa ccggcataac
     7141 ggagctaggg gccactcccg tgacgggcac ctccatgcgc gagatgatgg ggccgctcat
     7201 ggtagaccag accatccgcc aagccattca gcactgctgg ctgtccctgc ccgacgacga
     7261 acgctcgcca gaccgcgtgg aacaagaaat ccttcgcgtg gttcatcgcg cgcttcagaa
     7321 catgcgcgaa gacgcggcgg cgtttggttt ctcgaaagag gaaaagtcga gctgggaaga
     7381 acggttggcc cgcatgcgcg cacagtatcc gaaagcctac gagaagtgga ccgaggaaga
     7441 cgacagtttg ctcttgcaga agtttggcga aggtatgctc atggctgact tggcgaggtt
     7501 gtttcaacga cagcccagcg cgattcggtc gcatctggca aaactgggac tgatttgagg
     7561 cagagaagac ggcttgtcat gggtgggttt caatgcgtaa accgtatcgc cgggtacgtt
     7621 gtgcctttgg tgtcggtctc aattctcacc cataatggca cgctttgaca ttccacgcca
     7681 gcgactccgc aaccaaggca ttgccggagc gacatttaag aagccgggtg atgtggtgga
     7741 gtggctggcc gccgtccagg cccaagacta cgcaggcgcg aaatgggcac tcggactgcg
     7801 gttgcaagat gccacggacg acgatgtcga acaagccttc gccgagggcg ccatccttcg
     7861 cacccacctc ctgcgaccca cctggcattt tgtcacgcca gccgatattc gttggatgct
     7921 ggtactcacg gcgccgcgcg cccatgcggt caatgccgat atgtctcgca agctggaatt
     7981 ggacaacgcc ctctttaagc gcagcaacgc cgcgctggca agagcattac acggcggtcg
     8041 gcaacttacg cgggatgaac tgcgaggcgt gcttcagaaa gccggcatcg ccacggacgg
     8101 cgggttgcgc ctgggctatc tcctgatgcg tgccgaattg gacgggatgg tctgcagcgg
     8161 cgcacggcgt gggaaacaat tcacctatgc gttgctggat gaacgcgccc cgcacgccaa
     8221 gaccctgggg cgcgaggagg ccctggctga actcgctcag cggtactttg tgagccgtgg
     8281 gccagccacc gtgcaggatt ttgcgaagag gagctttacg catgcctaac cgtcccatag
     8341 ttatcctcga cgccccatca aaccttgggt tacgtccacc tgaacagggt gctgtaccgg
     8401 ggtgttacaa actgccttgg gcgcttcgcg accgaaagct tgtatccgcc attgatgcaa
     8461 cggatgcggg atcacttgtt ccgccaagat atcgtgctgc atggaatccc ggcgacggtg
     8521 atcgcaacgc agatgctatt gcggcgtttt ctacaatgct cgcagatcgc gttggctcca
     8581 ttcttacgcc gaatgggctc gcgtttgtgc tcggcggtga ttgcagcatc cttattggca
     8641 ataccctcgc cctcaaacgg cgcgggcgct atggcttggt atttcttgat gcccattccg
     8701 actttcggca cccgcagaat gcgcccgcga ttggtgctgc tgcgggtgaa gatctcgcga
     8761 ttgtgacggg gcgcggcgat ccacgcctca tcaacctcga aaatctcggc ccatacattc
     8821 gtgatgaaga cgtgatgctg attggcgtcc gtgatgatga cgagtgtctg cctgagcttg
     8881 cagaactcgg cattcacgtc attaccagtg caagattgga agaggtgggc gtacagcagg
     8941 cggcgacaca cgcgttggca actgcggcac aggcaaccca aggcttttgg gtacatcttg
     9001 atctagatat tgtggacgcg acagagatgc ccgcggttga ttgcccagag ccaaacgggc
     9061 tatcattctc tgcgctcacg gcgttgctca aatcactgct tgagtcaccg tcttgtgtgg
     9121 gccttgaact gaccatctat gatcctgacc tcgacccaac agggtactac gcagaccaag
     9181 tgatggcctg cattagcgct gtgtttcgca gttcataagg agattgccct gaatctactc
     9241 attgggttgt cttgcgcatt tcccgcgtac ctctatgcga gggagcgcac ttagaagtca
     9301 agttgaccgg ctggagaaac tgcgcatatg acccatgatc aaattaccgt agcgaatgcc
     9361 cctgccatat cgggattgag ctttcgccac tttcgaggag agagtgacta tccccacatg
     9421 gtagcagtca tcatgtccag tgcggacgca gacaaaatcg agcgcgcaga tacggttgac
     9481 gatatcgcca ataactacaa ccacctgacc aactgtgacc cctacaagga tatgatcttt
     9541 gcagaaatcg cgggtgaagt gattggctac tcgcgaggtt ggtggtggga tgaaccggcc
     9601 acgcgacgca tgtacggctt tgttggcttc cttgtgccgg cgtggcgacg caaaggtatt
     9661 ggtcgcgtga tgctgctttg gatggagaat cacctgcgtg gcatcgcaac gacacatcca
     9721 attgatcggg ccaggttctt ccaggtttcg gtctcgcaat ttcaagcagg tacgagcatc
     9781 atgctggagc gcgcaggcta ccggcccata cgctacttcc atgaaatggc gcggccatcg
     9841 ctgaatgaca tacccgacgt acctttaccg gatggtctcg aaatgcgccc ggcgttacct
     9901 gagcattatc gcttaatctg ggcgtctgtc gatgaaacga gtcaagacga atggggatat
     9961 acgggcgaag attatgaagc ctggctgaac aatatgcctc acttccaacc tcacttctgg
    10021 caaatcgcct gggatagtgc aaccgaacgg attgcagggc atgtgttgac ttttatcgat
    10081 catgcagaga acgagaggtt caaccgaaaa cgcggctata cagagggcat tggggtcggt
    10141 cgagcctggc ggcgtcgtgg cctagcccgt gcgctgatcg cacgcagcct acaagcacaa
    10201 aaagcagaag gcatgtccga gtcggctctc gttgccgaca gtgacagtat cagtggcgta
    10261 acccgcttgt atgaaagctg tggttttcga gttgtcaggc gcaacgccat ctatcgcaag
    10321 cccttttaag cgtaggcgcg gcccaggacg ggcgtgcagc ggattggcgc ttcttcatca
    10381 actcatcgag cgttttgccc tgtaagcagc ggctacagca actctacgaa ctcttccggc
    10441 gacaagtcgg catcgcgcag aatcttgcgc agcaggccgc gcccaacatc ctgttgcgcg
    10501 tgaacgggaa ccgttaccac tcggccatcc gcgtggtgca gccgaacgtg actgccgcgt
    10561 tgccgaacga tctgaaagcc tgccttctgc aagacacgga caacttctgc gcctcgcacc
    10621 atcggcaggg tcggcatcag acggtcacct tctgaatgcc aacgaactct ggaatagcct
    10681 cgcctgactc agcctgttct tcaaggcaga gttcgatcac ctcctgaatg ttcttcatta
    10741 gctcgtcgag cgttttgccc tgcgaatagc aaccgcggag ttgaggaact tctccgacga
    10801 aggtgccatc ctcgtcgcgc tcgatcagga cgtagaattc ctgcttccca cgttcgatat
    10861 cacctgccat gtgatttcct ccgcccggcg caggctcccg acaaagaaat tataccgcaa
    10921 gatcatcaga tgccggagcc gtttgctcac gccgcaccgg cgaccgtcac cgcctgcgac
    10981 tgccgtccat ctccagacac cctgccattg ccgcccgctg gctcctgccg ccggaactgg
    11041 ctcatgtata gatcgtagaa gaagccgcgc gcgtcgagca gctcccggaa cttgccccgc
    11101 tcgacgatct gcccatcgcg cagcaccagg atttgatcgg cgtgcttaac cgtgctcagg
    11161 cggtgcgcga tgacgaagct cgtgcggcca ctgagcagcc cctccagcgc ctgctggatc
    11221 agccgctcgg tgcgcgtatc gacgctcgac gtcgcctcgt cgaggatcag gatgcgcgga
    11281 ttcgccagcg ccgcgcgcgc gatcga
//
LOCUS       DBNHXB010000226         7244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1393951, whole genome shotgun sequence.
ACCESSION   DBNHXB010000226 DBNHXB010000000
VERSION     DBNHXB010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7244
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1393951"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="AC162G_09335"
     CDS             <1..921
                     /locus_tag="AC162G_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5076058.1"
                     /translation="GSLQDAEDLVQETLLRAWRRLDTYEGRASIRAWLYKIATNACLD
                     ALDKRSRRVLPSMLGPATEASQPFTPPVDEPIWLEPLPDHLIVETASDPEARYSLHES
                     VTLAFVAALQSLPPRQRAVLILRDVLDWRASEVADLLDLTVSAVNSALHRARVTLSRH
                     YHGSAAENVTWSPADDHVRALLDRYVSAWESADVAGLTALLKEDAVLNMPPSPSWYHG
                     RDAIRAFLQSMIFGSIVFPGSGAGRWRLTPTRANGQPGFGVYLRGPSGAYAAFGIIVV
                     AIDGDRVASITHFGTPTLLARFGLPDELAA"
     gene            complement(940..3252)
                     /locus_tag="AC162G_09340"
     CDS             complement(940..3252)
                     /locus_tag="AC162G_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5076059.1"
                     /translation="MTRRINPFVYGRYAPLSHFVGRRAEIDACYNALVGPVCGSVAIS
                     GEPRMGKTSLLRAVADVGSSEGWARPGTRTVFVYVDCQSISRFTPTRFWQRVLMLLGR
                     VLGDPAGRGASSAQTAPDPSPDSGQAVLSVTEALLAQERVDATEFESILHALRGQHIV
                     LALLLDEFEWVVKRDVKNLPVTRNFLSSLRALINHAPDTLSLIIASQQPLNVLCRGIT
                     NGEGSPFYNNFVFRRLHPLDASEVNELIERRLAGSGVQFAPQDRQYVAQVAGTHPFLV
                     QLACALIFDVRSRGAPIEYDVIGSRFEEEARHHFAQIWEISSPEERTVLLLVAWRWWA
                     RRLGTAEPADDRVYWALLRRYERDVISVVERGLATNAEDDPRPISSVFAWWIVNAVRT
                     GDTVVAPPDLAAVPDDEASHISQVIQQVRDTGSQIIRSMSVAPLSGWRAPLRPAEREH
                     PALPPADGGRPPQTAGEGGWQAAAPGPTPLGGAQAAVDNQRQERIGRYEVAGLLGEGG
                     MGAVHKAFDPSMSRYVAIKVVRTDALGSAADMLRIQQEAQLAGQLSHPNIVTLYDVMY
                     EPGRAYIIMEYLEGRTLAQLLRGDTLLPADLAISFVEQIADGLDFAHHHGIIHRDLKP
                     SNIIVLEDNRVKITDFGLAKLVHGASLTRPGHLIGTPNYMSPEQALGEPVSPQSDQFS
                     LAIAAYRMFTGRLPFGGDAFHAVMQQIVEADPRPIAPDELPEAAALSGILFKAMSKRP
                     QDRYATCRELAVALRLWLELAMAGEKTPIV"
     gene            complement(3372..6650)
                     /locus_tag="AC162G_09345"
     CDS             complement(3372..6650)
                     /locus_tag="AC162G_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5076060.1"
                     /translation="MNTRLANPYVVGAPLAGGHGFYGREDVFAFVTETLAVPSQNVIV
                     LYGQRRIGKTSLLHQLITRTGEGFHAVLFDLQGKGQHDLPALLRELSSAIARSLKLSA
                     PDPALFDSSRDTFREAFLPGVYGALDSRRLLILFDEFDVLGDESSSGEELAGSQLFGF
                     LRTWLEQDARLACVFVVGRRLDELPIHYRSLFKQAVFRRISVLPRHEAVELITQPAAG
                     EFSYSPEAIDAILELTSGHPYLTQLICHVIYKRLIAIPRRAVDADDVRDCLDEAMELG
                     TGGLDWFWEGLPRAERIMLSALAETLRTARPPSGSPRSDGGRERGAGSGPNGKSPLSA
                     REDEILQTLNRRRVRLLGVELTSARQRLLEWEIIARDGEAYRFAVDLVRRWIDREHPL
                     DRAQQDIDLISQRATRYFNNARDAHLSGDLALAIEDYRRALAANPHHFGARLGLALSL
                     HESGDLVAAIAEYEKAYQMDPSSTRDGLVSARLALGKAHQARAKLDAAEAEFEQVLRL
                     APSDDEAQRNLIDIWIKRGEDHLAANRYAQAVEAFSHALAIAPDDPPVQTRVKSIMRR
                     FSEAAEARGMWEMAFESLSRLAETLPDDEQLQSWLNETRLHWRAHNYFEMARRAHTVG
                     DHVTTIEECRRALEANPNHLQARLLLASVFYQAGDLSTAIEEYELAFRLDAGAAGPGL
                     AQARLQRAVELEAHDEIAAAVVDYERALEAHPSVETARRRLPAIWMRWGDDHLAAGRL
                     DQAVEHYRKALLVADQPQTLARPIKTKLAEFSQAQRSAGNWDAAVAAISRLRTDLMLR
                     DRESDGWLVGIWLQRGGGALLSGQYREAARAYKQALQTVADTSDAASLLARVKSDFFA
                     YADDRVRAEQSDAAIAALTMLIEVVGSDADSFGALSSAWLARGAMHLKHDRLDEAERA
                     FHRALELQPDNREAFARLTSVTERRRQIDIDRLRAEAAAHAGRRDWVKAEMLYRQLVF
                     DFQDEGSHQAFADVSEEVRLEELFNLAQSYQAKGEWNAAIPTWLEICHSRRDYVGLDG
                     QKAELLLAEAIRRDAGVAQASEWALARLKQRARQAWIVAVVFGLTIISLLVTILANGL
                     LRAR"
     gene            complement(6907..7161)
                     /locus_tag="AC162G_09350"
     CDS             complement(6907..7161)
                     /locus_tag="AC162G_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076061.1"
                     /translation="MVLENGAAQVAAWFGDVEVRRYPDTLVVTDENALLDYIFSLSTV
                     FNLPGQRKADLVQFVKKRFATQGGVFTITKDAGIIFGTRI"
BASE COUNT         1174 a         2399 c         2451 g         1220 t
ORIGIN      
        1 ggctcactgc aggacgcaga ggatctggtt caggagacac tgttgcgcgc ctggcggcgg
       61 ctcgatacgt acgaggggcg tgcgtcgatc cgcgcctggt tgtacaagat cgccaccaac
      121 gcctgcctcg acgcactcga caagcgctcg cggcgtgtgt tgccgtcgat gcttggccct
      181 gccaccgagg cgagccagcc gttcacgcca cccgtcgatg agccgatctg gctggagcct
      241 ctgcccgacc atctcatcgt cgagacagcg agcgatcccg aggcgcgcta ctccctgcac
      301 gagagcgtga cgctggcatt cgtcgcggcg ctgcagtctc tgccgccgcg gcagcgcgcc
      361 gtgctgatct tgcgcgacgt gctcgactgg cgcgccagcg aggtggctga cttgctggac
      421 ctcaccgtat cggcagtgaa cagcgcgctg catcgcgccc gcgtgacgct gtctcgacat
      481 tatcacggca gcgccgccga gaacgtgacg tggtcacctg ccgacgatca tgtcagggcc
      541 ttactggatc gttacgtgag cgcctgggag agcgccgacg tggcgggact gacggcgctg
      601 ctcaaagagg atgcagtact gaacatgccg ccctcgccgt cgtggtatca cgggcgtgat
      661 gcgatccgcg ccttcctgca atcgatgatc ttcggcagta tcgtctttcc ggggtctggc
      721 gctggccgct ggcgcctgac gccgacccgc gccaacggac aacccggttt cggggtctat
      781 ctgcgcggcc catccggagc ctatgccgcc ttcggaatca tcgtggtggc gattgacggc
      841 gaccgggtcg ccagcatcac tcactttggc actcccacgc tcctcgcgcg tttcggtctc
      901 cccgatgaac tggcggcttg agccgagttc gcacccagtt cacacgatcg gcgtcttctc
      961 gccggccatc gccagctcca gccacagccg aagcgcgacg gccagctccc ggcacgtggc
     1021 gtatcgatcc tgcgggcgct tcgacatcgc tttgaacaga atgccggaca atgcagccgc
     1081 ctcgggcagc tcgtccggcg cgatggggcg cggatcggcc tcgacgatct gctgcatcac
     1141 cgcgtggaac gcgtcgccgc caaacggcag gcgtcccgtg aacatgcgat acgcggcgat
     1201 cgccagcgag aactggtcgg actgcggcga cacgggctcg cccagcgcct gctcgggcga
     1261 catgtagttg ggcgtgccga tgagatggcc gggccgcgtc agcgaggctc cgtgcaccag
     1321 cttggccagg ccgaagtcgg tgatcttcac gcggttgtct tcgaggacga tgatgttgga
     1381 cggcttcaag tcacgatgga tgatgccgtg gtggtgcgcg aagtctagcc cgtcggcgat
     1441 ctgctcgacg aacgaaatgg cgaggtcggc cggcagcagc gtgtccccgc gcaggagctg
     1501 cgccagcgtg cgcccctcga gatactccat gatgatgtaa gcgcgccccg gctcgtacat
     1561 cacgtcgtac agcgtgacga tgttggggtg cgacaactgg ccggccaact gcgcttcttg
     1621 ctggatgcgc agcatgtcgg cggcgctgcc gagcgcgtcg gtgcgcacga ccttgatcgc
     1681 gacgtatcgg ctcatggacg gatcgaacgc cttgtggaca gcgcccatgc cgccctcgcc
     1741 cagcaggccg gcgacttcgt agcgaccgat gcgctcctgg cgctggttat cgacggctgc
     1801 ttgcgccccg cccagcgggg tggggcccgg cgcggcagcc tgccacccgc cctcgcccgc
     1861 tgtctgcggg ggtcgcccgc cgtctgcggg gggcagcgcc gggtgctctc gctcagcggg
     1921 ccgaagcggc gcccgccaac cggacagcgg cgcgaccgac atgctgcgga tgatctggct
     1981 gccggtatcg cgcacctgct gaatgacctg cgagatgtgg ctggcctcgt cgtcgggcac
     2041 ggccgccagg tcgggcgggg cgacgaccgt gtcccccgtg cgcaccgcgt tgacgatcca
     2101 ccaggcgaac accgacgaga ttgggcgtgg atcgtcttcc gcgttcgtcg ccagcccgcg
     2161 ctcgacgacg gaaatcacgt cgcgctcgta gcgccgcagc agcgcccagt agacgcggtc
     2221 gtcggccggc tcggcggtgc ccagccgacg cgcccaccag cgccacgcga ccaacagcag
     2281 gacggtgcgc tcctccggcg acgagatttc ccagatctgg gcgaagtgat ggcgtgcttc
     2341 ctcctcgaag cgcgagccga tcacgtcgta ttcgatcggc gcgccccggc tgcgcacgtc
     2401 gaaaatcaga gcgcaggcga gctgcaccag gaacgggtgc gtgccggcca cctgcgcgac
     2461 gtattgccgg tcctgcggcg caaactgcac gcccgagccg gcgagccggc gttcgatcag
     2521 ctcgttcacc tctgacgcgt cgagcggatg caggcgtcga aagacgaagt tattgtagaa
     2581 cggcgaacct tcgccgttgg tgatgccccg gcacagcacg ttgagcggct gctgagaggc
     2641 gatgatcagc gagagcgtgt ccggggcgtg gtttatgagc gcgcgcaggc tggacaggaa
     2701 attgcgcgtc accggcaggt tcttcacgtc gcgcttgacg acccattcga actcatctag
     2761 cagcaaggcc agcacgatgt gctgtccgcg cagcgcgtgc aggatcgatt cgaattcggt
     2821 cgcgtcgacg cgctcctgcg cgaggagtgc ttccgtcacg gagagcacag cctgccctga
     2881 gtctggcgaa gggtcgggcg cggtctgcgc gctgcttgcg ccccgcccgg cggggtcgcc
     2941 caggacgcgc ccgaggagca tcagcacgcg ctgccagaaa cgggtcggtg tgaagcggct
     3001 gatcgactgg cagtccacgt agacgaagac cgtgcgcgtg ccgggccgcg cccagccctc
     3061 gctcgatccg acgtcggcga cggcgcgcag cagcgacgtc ttgcccatgc gcggctcgcc
     3121 gctgatggcg acgctgccgc acaccgggcc gaccagcgcg ttgtagcatg cgtcgatttc
     3181 ggcgcggcga ccgacgaagt ggctgagcgg cgcgtagcgc ccgtacacga aagggttgat
     3241 gcgtcgtgtc ataggtgcgt ccctcggctc tcctgactct acacccgatc cattccccgg
     3301 ctcctgaagc ctgtcctgcg aagtccccga ccccgagcaa agcgaggggg agcgggtcat
     3361 ccgtgtgtgc gctaccgcgc tcgcagcagt ccgttcgcca gaatggtgac gagcaggctg
     3421 atgatggtca agccgaacac gaccgccaca atccaggcct gccgggcgcg ctgcttcagg
     3481 cgcgccagcg cccactcgct cgcctgtgcg acgccggcat cgcgcctgat tgcttcagcc
     3541 agcagaagct cagccttctg accgtcgagg cccacgtagt ccctgcggct gtggcagatc
     3601 tccagccagg ttgggatagc cgcattccac tcgcccttcg cctgatacga ttgcgccagg
     3661 ttgaacagct cttccagacg aacctcttcg ctcacgtcgg cgaacgcctg gtgtgagcct
     3721 tcgtcttgaa agtcgaacac gagctgccgg tacagcatct cggccttcac ccagtcgcgg
     3781 cgtccggcgt gggcggccgc ctcggcgcgc aagcgatcga tgtcgatctg cctgcgccgc
     3841 tcggtgaccg acgtcaatcg cgcgaaggct tcgcggttgt ccggctgcag ttcgagcgcg
     3901 cgatggaagg cgcgctcagc ctcgtccaac cgatcgtgct tgagatgcat cgcgccgcgc
     3961 gccagccagg cgctcgacaa cgcgccgaag ctgtcggcgt cgctgcccac gacttcgatc
     4021 agcatggtga gcgcggcgat ggcggcatcc gactgctcgg cccgcacgcg atcgtcggca
     4081 taggcgaaga aatccgactt gacgcgcgcc aggagtgacg cggcatcgct cgtgtcggcg
     4141 accgtctgca gcgcctgctt gtaggcacgc gcggcctccc gatattgccc ggacaacagc
     4201 gcccccccgc cgcgctgcag ccagatgccg acgagccagc cgtcgctttc ccgatcacgc
     4261 agcatgaggt cagtccgcag ccggctgatg gcggcgacgg cggcgtccca gttgcctgcc
     4321 gagcgctgcg cctggctgaa ctcggccagc ttcgttttga tggggcgcgc gagcgtctgc
     4381 ggctggtcgg cgacgagcag cgccttgcga taatgctcga cggcttggtc gagacgtccg
     4441 gcggccaggt ggtcatcgcc ccagcgcatc cagatagcag gcaggcggcg gcgcgccgtc
     4501 tcgacgctcg gatgcgcttc cagcgcgcgc tcgtaatcga cgacggcggc ggcgatctcg
     4561 tcgtgcgcct cgagctcgac cgcgcgctgc aggcgggcct gcgccaggcc cgggccggcg
     4621 gctccggcgt cgaggcggaa agccagctca tactcttcga tggccgtcga cagatcgcct
     4681 gcctggtaga acaccgaggc cagcagcagg cgagcctgca aatgattggg attggcctcg
     4741 agcgcgcggc gacattcctc gatcgtggtc acgtgatcgc cgacggtgtg cgcgcggcgg
     4801 gccatctcga agtagttgtg agcgcgccag tgcaggcgcg tctcgttcag ccaggattgc
     4861 agttgctcgt cgtcgggcag cgtctcggcc aggcgcgaca ggctctcgaa tgccatctcc
     4921 cacataccgc gcgcctcggc cgcttcgctg aagcgccgca tgatcgactt cacgcgcgtc
     4981 tgcaccggcg ggtcgtcggg cgcgatggcc aacgcgtgtg agaaggcctc gacggcctgt
     5041 gcgtaccggt tggcggccag atggtcttcg ccgcgcttga tccagatgtc gatcaggttg
     5101 cgctgcgctt cgtcgtcgct gggcgccagg cgcagcacct gctcgaactc ggcctcggcg
     5161 gcgtcgagct tggcccgcgc ctgatgcgcc ttgcccaaag ccaggcgcgc gctcaccaga
     5221 ccgtcgcggg tgctggacgg atccatctgg tacgccttct cgtattcggc gatcgccgcg
     5281 accagatcgc cggattcgtg cagcgacaga gccagcccca gccgcgcgcc gaagtgatgg
     5341 ggattagcgg cgagtgcgcg ccggtagtcc tcgatggcga gcgccagatc gccggatagg
     5401 tgcgcgtcgc gtgcattgtt gaagtagcgc gtggcgcgct ggctgatcag gtcgatgtct
     5461 tgctgggcgc ggtcgagcgg atgctcgcga tcgatccagc gccggacgag gtcgacggcg
     5521 aaacggtatg cttcgccgtc gcgcgcgatg atctcccatt ccagaaggcg ctgccgcgcg
     5581 ctggtcagct cgacgccgag caggcgcacg cggcggcgat tgagcgtctg caagatttcg
     5641 tcctcgcggg cggaaagcgg cgacttgccg ttcggcccgc tgcccgcccc cctctccctc
     5701 cccccgtcag aacgggggga gccggagggg gggcgcgccg tccgcagcgt ttcggccagc
     5761 gccgagagca tgatacgctc ggcgcgcggc aggccttccc agaaccagtc cagcccgccg
     5821 gtgccaagct ccatcgcctc gtccaggcag tcgcgcacgt cgtccgcgtc gacggcgcgg
     5881 cgcggtatgg cgatcaggcg cttgtaaatc acgtggcaga tgagctgcgt caggtagggg
     5941 tggccggagg tcagttcgag tatcgcgtcg atggcttcgg gcgagtagct gaactcgccg
     6001 gccgccggct gcgtgatcag ctcgacggct tcgtggcggg gcaggacaga gatgcgccgg
     6061 aacacggcct gcttgaacag cgagcgatag tggataggca gctcgtcgag ccggcggccg
     6121 acgacgaaga cgcaggcgag gcgcgcgtcc tgctcgagcc aggtgcgcag gaagccgaat
     6181 aattggctgc ccgccagctc ctctcccgaa gacgactcgt cgcccagcac gtcgaattcg
     6241 tcgaatagaa tcagcaggcg ccgggagtcc agcgccccgt agacccccgg cagaaacgcc
     6301 tcacggaacg tatcccggct ggagtcgaac agagccgggt cgggggcaga gagcttcagt
     6361 gagcgggcaa tcgccgacga gagttcgcgc agcagcgcgg gcaggtcgtg ctggcctttg
     6421 ccctgcagat cgaaaagcac ggcgtggaag ccctctcccg tccgcgtgat gagctggtgc
     6481 aggagcgagg tcttgccgat gcgccgctgg ccgtacagga cgatgacgtt ctgagaaggc
     6541 acggccagcg tctcggtcac gaatgcgaag acgtcttcgc ggccgtaaaa tccgtgccca
     6601 ccggcgagcg gcgcgccgac gacgtagggg ttggctaggc gcgtgttcat aggcgctcag
     6661 tctctgcgat ctgaagccac ggcacgttga aggtcgtaca aggacggtaa gctgcagcgt
     6721 atctcggtta agtgcacgca ttatagcatc cgagttgcgc ggtttgcgtt tcgtttaagt
     6781 tacagtagcg aaattttgac ctgtcaagtc cgtgctgtcc cgcccgcaat cgtgaaggta
     6841 gactcaaaca cttggattcc aggccgtagc tggccgaggc tggagaggcc cgaggagaca
     6901 aaagggctag atgcgcgtcc cgaagataat gcccgcgtct ttagttatcg taaacacccc
     6961 gccttgtgtc gcaaatcgct tcttgacgaa ctggaccaga tccgccttcc tctggcccgg
     7021 caagttgaac accgtcgata aggaaaagat gtagtcgagc agcgcgttct cgtcggtcac
     7081 aaccagcgtg tccggatagc gacgcacttc cacgtcgccg aaccatgctg cgacctgcgc
     7141 cgccccgttt tcgaggacca agcttgctat cgtgcgccgg acgaagggga tcgacgcaag
     7201 gattagtcaa ggaagttctg catgccctgg atgatccgct caag
//
LOCUS       DBNHXB010000227        15878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1729400, whole genome shotgun sequence.
ACCESSION   DBNHXB010000227 DBNHXB010000000
VERSION     DBNHXB010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 15878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15878
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1729400"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="AC162G_09355"
     CDS             complement(<1..283)
                     /locus_tag="AC162G_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076062.1"
                     /translation="MSSSPIDIVEAVTPQQRDEFIFFQWVPYKGNPYWVPPLISERRV
                     FLDKTKHPFHKHSEVALFLAKRAGQTVGTIAAIVNNRHNETHGENIGFFG"
     gene            complement(333..2816)
                     /locus_tag="AC162G_09360"
     CDS             complement(333..2816)
                     /locus_tag="AC162G_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5076063.1"
                     /translation="MNNHLRFAKYLFLVVVTPLLVMASLRIFKPIAASAGVDRVSIDA
                     GAPLIKQSDVLGEVPTNQIIIKYEASADLSGINAPAHANRMQALSAAAGVHLEYFREM
                     SGDAHVLRLPERMPVAQVEQIARRIAAMTGVEYVEPDRILQPLLTPNDPQYGSQWHYF
                     ETYGINAPAAWDITTGTNNVVVAVIDTGATNHADLSGRAVPGYDFISDVPTANDGNGR
                     DSDPSDPGVWVTSDESNTPGGPFQGCLVVDSGWHGTHVAGTIGANGNNSTGVAGVNWA
                     SRILPVRVLGKCGGYTSDIVDGMRWAAGLSVAGVPANAYPAEVLNLSLGGPGTCSSSQ
                     QSAISAIVAAGSTVVVAAGNENADASGSNPGNCSGVITVAATDRSGNRAFYSNYGSVV
                     EISAPGGETNVSDLNGILSTLNTGTTVPAFDTYVFYQGTSMAAPHVAGVASLLYSLSP
                     SLTPGQVGRILTTTITAFPVGSSCNTSICGAGIVNAGAAVNTLPRIAGTSPVTATTGG
                     PTFTMIITGANFASGATAKWNGSSRATQVLSSTQLSVTISAADIAVSGNYSLTVTSVH
                     ATYGAITTAARTFLVQGPASERVYLPLIMRNWPPIPPAPVLNAINNADGDGAYNVTWS
                     ASSTATSYTLEEDDNSSFSSPTAVYSGSGLLWVASGKAGGTYYYRVKATNSYGDSAWS
                     NVQSVTVLPQSGPTPGFWESQTGDEFYVSPDRANVLNFAIYVDVSGCGTYKITRLTPV
                     PISNNSFSFSGTFYASGTFDTSTLAHGTDGLSDYYIFGCGYVSGGPWSWTATWQNSSQ
                     PTVVYNIATPAMAVHVTTPHTAHPAAISD"
     gene            complement(2910..4313)
                     /locus_tag="AC162G_09365"
     CDS             complement(2910..4313)
                     /locus_tag="AC162G_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076064.1"
                     /translation="MRYAYLQRIGLVMLPVVLFVFFVGPIDVVVPPHIGYGFNVAYPD
                     SPRVAAIGFDWIKTLDDPPCPNRLPVKVLVRIDVDGHTDLNAWRNSVRALAQSRLGCL
                     EAFEIGNEPNLDASYGWAVAPNAVDYTAALCAAYEEIKSVSSSTKVISAGLAPAGRVA
                     GTWNGHPGHNGYYQDDREFLKEMIAAGAGNCLDAVGYHPYGFSADYDVEPDVDSPDPA
                     RKCVNGFCFRGVEKIYEIMQAHGLGSTQVWATEWGWITDPPAHCKNLPEWQGRLWQIV
                     SEQKQAGNLRGAFEYADAHWSWMGGMFVFNLDFNMADWYDECEQMRYYAVANHPAETV
                     LTGMPKRLLPTARLDVQVVSIHLLIAAADQPQTRTVTVAIGNSGLVTMTWSAQSDTGA
                     ALIPALTPISGTLAPEESQTINVVWTSGVRPVGVYTGTIVLSALPVDTINAPAAIPIT
                     LVVADRVYENTLPLVIK"
     gene            complement(4439..4843)
                     /locus_tag="AC162G_09370"
     CDS             complement(4439..4843)
                     /locus_tag="AC162G_09370"
                     /inference="COORDINATES: protein motif:HMM:NF048130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR5076065.1"
                     /translation="MRRLYPALVAVLVVALILPTVAAAAPAESGGFSYQIRLGDTLYG
                     IARKFGVSPQAIASANGLVNPNLIYAGYWLWIPDAPYYPPYGCRYSHYVQPGQTLYRL
                     GRWYGVSPWAIASANGIYNLNLIYAGTTLCIP"
     gene            complement(4946..6187)
                     /locus_tag="AC162G_09375"
     CDS             complement(4946..6187)
                     /locus_tag="AC162G_09375"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR5076066.1"
                     /translation="MADTKATATLLRTVPFLRGLSGADYAALARRSRERAFKAGKMIF
                     REGSQGTTLWIIKDGEVDIVKGRGRDEVTLVTRRAGEFFGEMGIIEGTKRSATTRAHT
                     DVTLIEVPGDVVMKTLLKHPPVLLETTRRLSSNLRQADTKMIQGLKKKNAELQRAYKA
                     LQQAQDEIVEKKRLERELELARELQDSLLPRELPSLPGLQFAGRSRPAEAVGGDFYDV
                     IPIRSNLFGLLIASIAGQGLFSAIFMALTRALVVSEGQRQEHPHLVASNVHRLLLQLA
                     KPTMPVSMFYGVIDAREGTVTYVNAGHTAPLVRSADGSVAALAGAGTQLAASLRVQVD
                     ERSTTLQAGQTLVLYTDGLTRTENPAGEPFGLNRLKAVLAGEVRSAGMLVGSVFAAVD
                     AHRGEAAQRYDQAILVAHVKD"
     gene            complement(6294..7025)
                     /locus_tag="AC162G_09380"
     CDS             complement(6294..7025)
                     /locus_tag="AC162G_09380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR5076067.1"
                     /translation="MTKRPVIGITALATRYIDPPHSPMYALNQRYVMAVEASGGAPLM
                     VPPDLDETSMQAIFERLDGWVLSGGGDVDPAYYGESPHAALNEVNAERDRLELSFARQ
                     AVEADKPVIGICRGVQVLNVALGGSLVQDIPTQQADSLPHSFDGTRVARDYMAHPVRI
                     ESGSLLREVMGVEQAGVNSWHHQSVKRVAASLAVVAQSPDGIIEAVEMPGRRFVIGVQ
                     WHPEWLYDHQPEMKRLFEALVQAAK"
     gene            complement(7090..7449)
                     /gene="rplS"
                     /locus_tag="AC162G_09385"
     CDS             complement(7090..7449)
                     /gene="rplS"
                     /locus_tag="AC162G_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR5076068.1"
                     /translation="MNVLDTLTKKKTDLPPLRPGDTVRVHALIVEGNRERVQVFQGTV
                     IRLRRSGAQANFTVRRIASHGVGVERTFPLHSPRIEKIEVVRRAKVRRAQLYYLRGLR
                     GKASRLKEATTAKESES"
     gene            complement(7570..8109)
                     /locus_tag="AC162G_09390"
     CDS             complement(7570..8109)
                     /locus_tag="AC162G_09390"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5076069.1"
                     /translation="MQQETVLASRRIYDGRVVSLRVDDVRTSRSLQTMREIVEHHGAV
                     ALVALDDEDRVLFVKQYRHAIGRVTTEIPAGTLEPGEDPLASAARELAEETGYSASQF
                     ERIGGIYPSPGFCTEHIHLYLARGLTRGVAHPEDDEHIVVESVAWAEAIRRVRAGEID
                     DAKSVCALLLADAARSREL"
     gene            complement(8171..8788)
                     /locus_tag="AC162G_09395"
     CDS             complement(8171..8788)
                     /locus_tag="AC162G_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR5076070.1"
                     /translation="MKSGRNRLGVLVSGLAIALLLITGCERSAAQDPLAGTSTAAAQI
                     AETPSSTPVVVAVTPETTPAVIAAVTDTPVPTPVPPTPTQAPPTATATAAPGGTTTYT
                     VKSGDWLFSIGRRFGVNPYSIAAANNIPPPYTIYPGQVLRIPTSGGGTTPPPACSSGK
                     THTVQLGENLYRLSLRYGTTVQAIATANNISNPNVIFAGQVLCIP"
     gene            8956..10242
                     /locus_tag="AC162G_09400"
     CDS             8956..10242
                     /locus_tag="AC162G_09400"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR5076071.1"
                     /translation="MSNDTLTVTDNRTGKSYEIPITDETIRATDLRQIKISPDDFGLM
                     SYDPAFMNTASTKSAITYIDGDKGILRYRGYPIEQLAEKSTYLETAYLILFGELPNAS
                     QLESWTYNVTHHTFLHENIKTLMEAFRYDAHPMGMFISTVAALSTFYPAAYQVDDPDV
                     RMKQIYRLIAKVPTIAAFSYRHSRGLPYVYPDNDLSYTGNFLNMLDKMTQVKYQPKPV
                     LERALDVLFILHADHEQNCGTNAMRAVGSSRVDPYSAMAGAAAALYGPLHGGANEQVL
                     RMLTQIGSTSSVPAFIDKVKQGEGRLMGFGHRVYKNYDPRATIIKQTADAVFEVTGSN
                     PLLDIALELEQIALKDEYFISRKLYPNVDFYSGIIYQAMGFPTEFFPVLFAIPRTVGW
                     LAQWVEMLEDQEQKIARPRQVYLGRDIRDYVPLERR"
     gene            complement(10320..13664)
                     /locus_tag="AC162G_09405"
     CDS             complement(10320..13664)
                     /locus_tag="AC162G_09405"
                     /inference="COORDINATES: protein motif:HMM:NF021635.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HIR5076072.1"
                     /translation="MARLPPSAPALLVLIAVGLVLAAPLLQGETLCTDDGALHIYRTV
                     ALDRALRDGLLYPRWFPDLAYGYGFPFFVYREPLSYYALEALHLLGLSVPTAFNVTMA
                     GGVVVAGIAIYLLGRDIFGPRGGMLAGVVHMAAPYVLIGPLVRGNLPEVIAFALMPLI
                     LFFFRRLIVYGHTRYFVASTASYAALFLTHNISSLLFTPFLLVYIALLGWVQVADSRW
                     QMADGRSPIADSPASPRDRSSIISHVRYAIRDTRYAICDTRYALLAVALTLGLTAFFW
                     LPALAEQNEAQLYLTHSTRGNDYHFNFISLEELLGGPGSFDPNLLNPPLRISFGWAQL
                     VVAALGALGYRRAQNREQRATVIAAAVAAITFTLMALPITLPLWERLPLIRFVQFPWR
                     FVGRAMLPTALLAAASLSPPRSINHKDTKTRSQLRVFAPLRLRSGQASWLVVTVFGTV
                     VLVALLFTAPLSYPRVCPTPHALDINDVFAYERLSGHVGVDPLGAYLPVTVIERPGGS
                     PLEAMYSAGRAIERFDASALPGGARVFDAQYGPNRADVLVSSPTAFTATYLAFAFPGW
                     NAQIDGHPIAIRPSYPNGLVTFDVPAGEHRIHVEFGDSPQRALADGISLIAVVAMIAA
                     VVIHWRSRRSLIAYRLLLIASGARHASRFTLSILLALLVYVVVKSLLIDPGLTPLRAT
                     RLIGDALNGIAHPPDATFGDELRLLGYTATPSTARPGERFRVDLYWKALKPLTANYQA
                     EVSIADTDGWLWSPKHADRPRDYNSFPSMPEWPLDGYAVDSFEVEVLPGAPPGEYDLV
                     VQVFDRETLAPLLTGTLPAPGRVAASIGKIRVDRATRSFDADELRIYGGERHELDDDM
                     TLLGYNIDRVEGVPGESILLTFFWQADRKPQTDRSFRIELVDSSGNVLSSSVLPVGNE
                     SYRTSHWEAGEQVITLASLRVPAAAPSGDYHWRAVLLDDAARPAYDVELQPTLRVTAP
                     ARTFDVPPIENRIDVGVGDFATLLGFNVAGDAFAPGDTIDLTLFWQPRAETSTSYKVF
                     VHVLDEAGHIVAQADAVPVNGERPTTGWLPHEVIPDRHTIPLPGDLVGGLYRIQAGLY
                     DVDGGARLKTSEGADSIILADIDVKE"
     gene            complement(13732..>15878)
                     /locus_tag="AC162G_09410"
     CDS             complement(13732..>15878)
                     /locus_tag="AC162G_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076073.1"
                     /translation="LLFAMSFIVFLTPAAKKFDRYVLPAIPPLIIAAAWGIHQLTDKP
                     TSRLSRLLSPAAILLQAILLLSVIPYPLRAYNPLLGGAAGARDRIAVGWGEGLIAAAR
                     WVAERQPGATVAIGGLPNLVPLYAGRIATLNDAGLAAADHIIFTISEVQQAPDFFASL
                     AQRGTLVHTIRSGGIDTTWVYRNDRHAEQAAWIRRRAGPRDAILLDAPTPLAHLLDPL
                     PARILPPNVTPEGISSTMDALHEYEHILYISTAAATPVVRRDVCAWLDANARLESETP
                     LPGATARVYAPENQTSTSLTPFVVQFDGSLALLGLEMLTDTAAYPDSIVVAARWLTLA
                     PPATDYAATFEVSDASGDGWVRIGGLLRNASDFAPTEWKPGEVVEQAFSAQVPPWLAP
                     GSYRLRFSVDRADGSRAGLVDASGAFTGSAPVLASVTIEPSRQFANPTDILTHQRIEH
                     AWPGRVELIGADILSYVPSTGDLFFATMYWRALRDGLDPGMELRWSLQPTFTTDVPPL
                     EWQTPLAPHTPARLRAGDIVAARYAVRLPLGLPDGRYHLRLSIDGETLDVATNIDISH
                     VEREFDLPPGAVAVGSLGTFDVYLRRPLPAHLKPGESLQVNLASRAEEEVLVAYSVSV
                     RLIAPDGQTVAQSDSWPQQGAWPTTNWVRHQIVEDTHTMTLPSDAPPGVYRVAISMYD
                     SLDGSALPARDANRPAAPDRHLKLGTLQVSAP"
BASE COUNT         2995 a         4809 c         5124 g         2950 t
ORIGIN      
        1 acccgaagaa gccaatgttc tcgccgtgcg tctcgttgtg gcgattgttg acgatcgcgg
       61 cgatggtgcc gacggtctgc ccggcgcgct tcgcgaggaa cagcgcgacc tcgctgtgct
      121 tgtggaaggg atgtttggtc ttgtcaagaa agacgcgtcg ctcgctgatg agcggcggca
      181 cccagtaggg gttgcctttg tagggcaccc actggaaaaa gatgaattcg tcgcgctgct
      241 gcggcgtgac ggcttcgacg atgtcgatgg gtgaggatga catatagtgt cctcgttgcg
      301 tcgcatgttt gtagtctttg ttggctattc gactaatcgg agatggcagc cggatgggcc
      361 gtgtgcggtg ttgtgacgtg cactgccatg gcaggagtgg cgatgttgta aacgactgtc
      421 ggctgcgaac tgttctgcca ggtcgccgtc cagctccatg gcccgccact gacgtatccg
      481 cagccgaaga tgtaatagtc gctcaaccca tccgtgccgt gggccaacgt ggatgtatcg
      541 aacgtgccgg aggcgtagaa cgtcccgctg aatgagaaac tgttattgga gatcggcaca
      601 ggagtgaggc gcgtaatctt gtatgttccg cagcccgaga cgtcgacgta aatggcaaag
      661 ttcagcacgt tggctctatc gggcgagaca tagaactcat cgccggtttg gctttcccag
      721 aaacccggtg tagggccgga ctgcggcaac accgtcacag actgcacgtt gctccaggcg
      781 ctgtcgccgt aggagttggt tgccttcacg cgatagtagt acgtgccccc cgccttgccg
      841 ctggccaccc acaggagccc ggagccgctg tagactgccg tcgggctgga gaacgacgag
      901 ttgtcatcct cctccaatgt gtacgaagtc gccgtactgg atgcgctcca cgtcacattg
      961 taggctccgt cgccgtcggc gttgttaatt gcgttgagca cgggcgcagg cggaatgggc
     1021 ggccagttcc gcataatcag cggcaggtac acgcgctcac tcgctggccc ctgaactaga
     1081 aacgtgcgcg cggcggtcgt gatcgcgcca taggtggcat gaacgctggt caccgtaagg
     1141 ctgtagttgc ccgacacggc aatgtcggcc gccgaaatgg tgacgctcaa ttgggtgctg
     1201 ctgaggacct gcgtcgcccg gctcgatccg ttccatttcg ccgttgcgcc gctggcgaag
     1261 ttcgcgcccg tgatgatcat cgtgaaggtc ggaccgcccg tcgtcgccgt gaccggactc
     1321 gtgccggcga tgcgcggcag cgtgttgacc gccgcaccgg cgttgacaat gcccgcgccg
     1381 caaatggatg tattgcagct actgccgacc gggaaggccg tgatggtagt cgtcagaatc
     1441 cgccccacct ggccgggcgt cagcgatggg ctgagcgaat acaacagcga tgccacaccg
     1501 gccacgtgcg gcgctgccat gctagtacct tgataaaaga cgtaggtatc gaatgcggga
     1561 acagttgtcc cggtgttgag cgtcgaaagt atgccgttca ggtcggacac attggtctcg
     1621 ccgcccggcg cgctgatctc gacgaccgat ccatagttac tgtagaaggc gcggttgccg
     1681 gaccgatcag tggccgcaac agtgatcacg ccgctgcagt tacctgggtt ggagccgctg
     1741 gcatcggcat tctcgttgcc ggccgcgacg acgaccgtcg aaccggcggc aacgatcgcg
     1801 ctgatggcgc tctgctgaga cgaactgcat gtgcctggcc cgcccaggct caggttgagc
     1861 acttctgccg gataagcatt ggccggcacg ccggcgacac tcagcccggc cgcccagcgc
     1921 ataccgtcga cgatgtccga tgtgtagccg ccgcacttgc ccagcacgcg caccggcaga
     1981 atcctcgatg cccagttgac gccggccacg cccgtgctgt tgttgccgtt cgcgccgatc
     2041 gtgccggcga cgtgcgtgcc gtgccaaccg ctgtcgacga ccaggcagcc ctggaagggg
     2101 ccaccgggag tgttgctttc gtcagaagtg acccaaacgc ccggatcgct tgggtcactg
     2161 tcgcgcccat tgccgtcgtt tgcagtgggt acgtcgctga tgaaatcgta gccgggcacg
     2221 gctcgaccgc tcagatcagc gtggttggtg gcgccggtgt cgatcacggc gacgacgacg
     2281 ttgttcgtgc cggtggtgat atcccacgcg gcgggcgcgt tgatgccgta cgtttcgaag
     2341 tagtgccact gcgaaccgta ctgcggatcg ttgggcgtga gcagaggctg caagatacgg
     2401 tccggctcga catactccac gccagtcata gccgcgatgc gcctggcgat ttgctcgacc
     2461 tgagccaccg gcatccgttc gggcaggcgc agcacgtgcg catcgcccga catttctcga
     2521 aagtactcca ggtggacccc ggctgccgcg ctcaatgcct gcatccggtt cgcgtgcgcc
     2581 ggcgcattga tgccgctgag atcagcggat gcctcatatt tgatgataat ctgattggtc
     2641 ggcacttcgc ccagcacgtc actttgcttg atcagaggag cgccggcgtc gatggaaact
     2701 cgatcgacgc cggcgcttgc cgctatcggc ttaaagatcc gtagcgaggc cataaccaag
     2761 agcggtgtga caactacgag gaaaagatac tttgcaaatc gcagatggtt attcatgttg
     2821 ggttttcctt tcccgaatgt ctactgcgta ctgaaatgcg gtctgggccg ccgggcctca
     2881 cgagccgatg tagttcacga tggtcgcgtc tacttgatga ccagaggaag agtgttctcg
     2941 tatacccgat cggcgacaac cagcgtgatc ggaatagctg cgggtgcatt gatcgtatcg
     3001 acaggcaggg cactcagcac gattgtgccg gtgtacaccc cgacgggtct cacgccgctc
     3061 gtccacacga cgtttatcgt ctgggactct tctggagcaa gcgtgccgct gatcggtgtg
     3121 agagccggga tcagtgctgc gccggtgtca ctctgcgccg accacgtcat cgtcaccaat
     3181 ccggagttgc cgatcgcaac tgtcaccgtt cgcgtctgcg gctggtcggc ggcggcgatc
     3241 aggaggtgga tcgacacgac ctgtacgtcg agcctggcgg tcggtaggag gcgtttgggc
     3301 atgccggtga gcacagtttc ggcaggatgg ttcgcgacgg cgtagtaccg catctgctcg
     3361 cactcgtcgt accagtcggc catgttgaaa tcgagattga agacgaacat gccacccatc
     3421 caggaccaat gggcgtccgc gtactcgaac gcgccgcgca gattgccagc ctgcttctgc
     3481 tccgatacga tctgccacag tcgcccctgc cactccggca ggttcttaca gtgcgcaggt
     3541 ggatcggtga tccagcccca ttccgtggcc cagacttgcg tgctgcccag gccatgcgcc
     3601 tgcatgattt cgtaaatctt ctccacgccg cggaagcaga acccgtttac acatttgcgc
     3661 gccggatcgg gagagtccac atcaggctct acgtcataat cggcgctgaa gccgtaggga
     3721 tggtagccga ccgcgtcgag acaattgcct gcaccggcgg cgatcatttc tttcaggaac
     3781 tcacgatcgt cctggtagta gccattgtgg ccgggatggc cgttccaggt tccggccacg
     3841 cggccggccg gggccagccc cgccgagatc actttagtgc tgctcgacac gctcttgatt
     3901 tcttcgtacg cggcgcacag agccgcagtg tagtcgacgg cattcggcgc gacggcccag
     3961 ccgtagctcg cgtcgaggtt aggctcgttg ccgatctcga acgcttcgag acagccgagg
     4021 cgcgattgcg cgagagcgcg taccgagttg cgccacgcgt tgaggtcggt atgaccatcg
     4081 acgtcgattc gcacgagtac cttgaccgga agtcgattcg ggcatggcgg atcgtcgagg
     4141 gtcttgatcc aatcgaagcc gatcgcagcg acgcgcggcg aatcggggta ggctacattg
     4201 aacccatagc cgatatgagg tggcacaact acatcgatcg gaccaacaaa aaacacgaac
     4261 aacacaacgg gcagcatcac cagcccgatg cgctgcaaat aggcgtatct caaatcgacc
     4321 ctctccaaat cgtcaaggcg gtggcgatct taacaggaga ggagatcgat tagattgagc
     4381 caatagtcac cgacgggcga ctcccaccgt gcgtcccgta caggcagttg tgtttgcctt
     4441 acgggatgca cagcgtcgtg ccggcgtaaa tcaggttcag gttgtagatg ccgttggccg
     4501 aggcgatcgc ccacgggctg acgccatacc agcgcccgag gcgatacaac gtctggcctg
     4561 gctgcacgta atgggaatat cggcatccgt atggcggata atatggcgcg tcgggtatcc
     4621 acagccagta gccggcgtaa atcagattcg ggttgaccaa cccgttcgcc gacgcgattg
     4681 cctgtgggga cacaccgaac ttgcgagcga ttccgtacaa ggtatcgccc agccgtattt
     4741 gatacgaaaa cccgcccgac tccgccggcg cggccgccgc caccgtcggc aagatcaacg
     4801 ccacgaccaa gacggcaacg agtgccgggt acaatcgacg catggcaaat cctccttgtt
     4861 aggttcaatg gcaggtaacg cgccacctgc aggataaact ccgctcgcat tatacatccg
     4921 gttgtgcaaa tgggatcaag tgtgttcaat ccttcacgtg tgcgacgaga atcgcctggt
     4981 cgtagcgctg agccgcttcg ccgcgatgcg cgtcgacggc cgcgaatacg ctgccaacga
     5041 gcatgccggc cgacctcacc tcgccagcca ataccgcctt caatcgattc aatccgaaag
     5101 gttcgccggc tgggttttcc gttcgggtaa gcccatcggt gtacagcacc agggtctggc
     5161 cggcctgcag cgtcgtcgag cgctcgtcca cttgcacgcg cagcgatgcg gcaagctgtg
     5221 tgcccgcgcc cgccaacgcg gcgacggagc cgtcggcgct gcgcaccaac ggtgcagtgt
     5281 ggccggcgtt gacgtacgtc acggtccctt cgcgcgcatc gattacgccg tagaacatgc
     5341 tcaccggcat cgtcggtttg gcgagctgca ggagcagacg atgcacgttc gacgcgacga
     5401 ggtgggggtg ctcctggcgc tggccctcgg agacgaccag cgcgcgggtg agtgccatga
     5461 agatcgccga aaacagcccc tgcccggcga tgctggcgat gagcagaccg aagagattcg
     5521 agcggatcgg gatgacgtcg tagaagtcgc cgcccacggc ctcggccggc cggctgcgcc
     5581 cggcgaattg gagacccggc aggctgggca actcgcgtgg cagcaggctg tcctgcagct
     5641 cgcgagccag ctccagctcg cgctccaggc gcttcttttc aacgatctcg tcctgggcct
     5701 gctgcagggc cttgtaggcg cgctgcaatt cggcgttctt cttcttcaaa ccctggatca
     5761 tcttggtgtc agcctggcgc aggttgctgc tgagccgacg cgtggtctcc aggagcacgg
     5821 ggggatgctt cagcagcgtc ttcatcacca catcgccggg cacctcgatc agcgtcacgt
     5881 cggtgtgggc gcgcgtcgtc gccgagcgct tcgtcccttc gatgatgccc atctcgccga
     5941 aaaactcgcc ggcgcgccgc gtgacgagtg tcacctcatc cctgccccgc cccttgacga
     6001 tatcgacttc gccgtcttta atgatccata gcgtcgtgcc ctgcgagcct tcgcggaaga
     6061 tcatcttgcc tgctttgaag gctcgctctc gcgaccgtcg cgccagggcc gcatagtctg
     6121 cgccggacag acctcggagg aacgggactg tcctgaggag agtcgcagtc gctttagtgt
     6181 cggccatcgt ttttcccctt gcactgggag gcagattcgt gccaaggcaa atcttatcac
     6241 gaatgacgcc tcaaaacaaa cctcccgcag gttcacaacc tgcgagaggt ttctcacttc
     6301 gccgcctgca cgagcgcctc gaataggcgt ttcatctccg gttggtgatc atagagccat
     6361 tcaggatgcc actgcacgcc gatgacgaac cggcggccgg gcatctcaac ggcttctatg
     6421 atgccgtcgg gtgactgagc gacgaccgcc aagctggcgg ctacgcgctt caccgactgg
     6481 tgatgccacg agttaacgcc ggcctgctcc acgcccatca cttcgcgcag gagagagcct
     6541 gattcgatgc gcaccggatg cgccatgtaa tcgcgcgcca cccgggtgcc gtcaaatgaa
     6601 tgaggcaggg aatcggcttg ctgggttgga atgtcttgca ccagtgatcc gcccaacgcc
     6661 acgttcagta cctgcacgcc ccggcaaatg cctatgaccg gtttgtctgc ctccaccgcc
     6721 tggcgcgcga atgacagctc cagccggtcg cgctcggcgt tcacctcgtt cagggcggcg
     6781 tggggtgatt cgccgtagta tgcagggtcg acgtcgccgc ccccggatag cacccatccg
     6841 tcgagtcgct cgaagatcgc ctgcatcgac gtctcgtcga ggtcgggcgg caccatcagg
     6901 ggcgcgccgc cggacgcctc gaccgccatg acgtatcgtt gattgagcgc gtacatcggt
     6961 gagtgcggtg gatcgatata gcgtgtggcc aaggcggtga tgccaatcac cgggcgtttc
     7021 gtcatcggtt tctccaacga tcaaaaaagg cgtagatgaa gctctacgcc cctcggtctg
     7081 accttcggat tacgactcgg attccttcgc cgtggtggct tccttcaggc ggctggcctt
     7141 gccgcgcaaa ccgcgcagat agtacaactg cgcgcggcgc accttggcgc ggcgcacgac
     7201 ttcaatcttc tcgatgcgcg gtgaatgcag cgggaacgtg cgctcgacgc cgacgccgtg
     7261 cgaggcgatg cggcgcaccg tgaagttcgc ctgcgcgccg ctccggcgca gccggatcac
     7321 cgtgccctgg aaaacctgca cgcgttcgcg gttgccttcg acgatcagcg cgtggacgcg
     7381 caccgtgtcg cccggtcgca atgggggcag gtcggtcttc tttttggtca gggtgtccag
     7441 cacattcatg gccttcaagt cctttggtga tacatatgct tgcccgggat gccgtctgtt
     7501 ccggatagcg gcataacatt ataccacgcc gatcgtgttc cgacaaggtc aatttttctg
     7561 gtgagcgggt tacagctctc tactgcgtgc tgcgtcggcc agcagcagcg cgcagacgct
     7621 tttcgcatcg tcgatctcgc ctgccctcac ccggcgtatc gcctcggccc aggctacgga
     7681 ttcgaccacg atgtgctcat cgtcttccgg atgggcaacg ccgcgcgtca ggccgcgcgc
     7741 caaatagagg tggatatgtt ccgtgcagaa tcccggactg ggatagatgc cgccgatgcg
     7801 ctcgaactgc gacgccgagt agccggtttc ttcggccagc tcgcgcgccg ccgacgccag
     7861 gggatcttcg cccggctcga gcgttccggc cggaatctct gtcgtcactc ggccaatagc
     7921 atgccggtac tgctttacga aaagcacccg gtcttcgtcg tcgagtgcga ccagcgccac
     7981 agcgccgtga tgctcgacga tctcgcgcat cgtctgcaga ctgcgcgacg tgcggacgtc
     8041 gtcgacgcgc aggctgacga cgcggccatc gtagatcctg cggctggcga gcacagtctc
     8101 ttgctgcatg caacctccca aagaaaagcg gctaccgtgc ccggtagccg cgcggtattt
     8161 tgctgccgat tcacggaatg cacaacacct ggcctgcgaa aatgacgttg ggattggaaa
     8221 tgttattcgc cgtagcgatg gcctgcaccg tggtcccgta cctgagcgag aggcggtaca
     8281 ggttctcgcc gagctgcacg gtgtgcgtct tgccactgct gcatgccggc ggtggagtgg
     8341 tgcctcctcc gctcgtcggt atcctcagga cctggccagg gtagattgtg tagggcggtg
     8401 gaatgttgtt cgccgctgca atcgaatagg gatttacgcc gaacctccgg ccgatgctga
     8461 acagccagtc gccggatttg acggtatagg tggtcgtgcc gccaggggca gccgtggcgg
     8521 ttgccgtggg tggcgcctgc gtaggtgttg gcggaacggg cgtgggtacg ggcgtgtctg
     8581 tcactgccgc aatcaccgcg ggggtcgttt caggtgttac ggcgacaacg accggggtcg
     8641 agcttggcgt ttcagcgatt tgggctgctg ccgtgctcgt gccggccagc ggatcctgcg
     8701 ccgcgctgcg ctcgcagccg gtgatgagga gcagggcgat cgcaagcccg cttactaaca
     8761 cgccaagccg gtttctgccc gatttcatgg tgcgcctcct taacagggcc gccagatcat
     8821 gccttggcct gattggcaca caggataaca taagccagac atagagtcaa gttgcagtat
     8881 aatggctaca ttgcgagccg agctttcggc gaccgcgcct ccagacataa caccttttga
     8941 ggagaagaat ctgtcatgtc caacgatact ttgaccgtca ccgacaacag gacgggcaag
     9001 tcctacgaga tccccattac ggatgagacg atcagagcca cagatctgcg tcagatcaag
     9061 atttcgccag atgacttcgg cctgatgagc tacgatccgg cgttcatgaa cacggcgtcg
     9121 accaaaagcg ccatcaccta catcgatggc gataagggca tactgcgcta ccgaggctat
     9181 ccaatagaac agttggccga gaagagcact tacctcgaaa ccgcctatct gattctgttc
     9241 ggcgagctgc ccaacgccag ccagctcgag tcgtggacgt acaatgtcac gcaccacacc
     9301 ttcctgcacg agaacatcaa gacgttgatg gaggccttcc gctacgatgc ccacccgatg
     9361 ggtatgttca tcagcacagt ggcggcgctg tccacgtttt atcccgcagc gtaccaggtc
     9421 gacgatcccg acgttcgcat gaagcagatt taccgcctga tcgccaaggt gccgacgatc
     9481 gccgcgttct cctaccggca cagcaggggt ctgccgtacg tctatcccga caacgacctg
     9541 agttacaccg gcaactttct gaacatgctg gataagatga cccaggtgaa gtaccagccg
     9601 aaaccggtgc tcgagcgcgc gttggacgtg ttgttcatcc tgcatgccga tcacgagcag
     9661 aactgcggca caaacgcaat gcgcgccgtc ggatcgtcca gggtcgatcc ctattcggcg
     9721 atggccggcg cggcggcggc gctatacggc ccgctgcacg gcggggccaa cgagcaggtg
     9781 ctgcgcatgc tgacacagat cggcagcacg tcgagtgtcc ccgccttcat cgacaaagtg
     9841 aagcagggcg aggggcgact gatgggcttc ggccaccgcg tgtacaagaa ctacgatccg
     9901 cgcgcgacga tcatcaagca gacggccgac gccgtcttcg aggtgactgg ctccaacccg
     9961 ctgctcgaca tcgcactgga gctggagcag attgccctga aggatgagta cttcatttcg
    10021 cgcaagctat acccgaacgt cgatttctac tcgggtatca tctatcaggc gatgggcttc
    10081 cccaccgagt tcttcccggt gctattcgcc atcccgcgca cggtcggctg gctggcgcaa
    10141 tgggtcgaaa tgctggaaga ccaggagcaa aagatcgcgc gcccgcggca ggtctacctg
    10201 ggtcgtgaca ttcgcgacta cgtcccattg gagcggcgat agggtgtatt cggcaagaat
    10261 gatagcgtga cttgagtcgc ctgatcggcg caagcccgcc atgcgcactt gtggcgggct
    10321 tattctttca cgtcgatgtc ggccagaatg attgagtctg cgccttcgct cgtcttcaag
    10381 cgggcgccgc cgtccacgtc atacaacccg gcttgaattc ggtacaggcc cccgaccaaa
    10441 tcgccgggta gcgggatggt gtgcctgtcc ggaatgactt catgtgggag ccaacccgtt
    10501 gtcggacgtt caccgttgac cggcactgca tctgcctgcg cgacgatgtg gcctgcctcg
    10561 tccaggacat gcacgaagac tttgtaagat gtcgaggttt cagcgcgcgg ttgccagaac
    10621 agcgtcaagt caatcgtgtc acccggcgcg aatgcgtcgc cggccacatt gaagccaagc
    10681 agtgtcgcaa agtctccgac ccccacatcg atccgattct cgatcggcgg cacgtcgaac
    10741 gtccgcgccg gcgcagtcac gcggagtgtt ggctgcaact cgacgtcgta ggcgggtcgc
    10801 gccgcgtcgt cgagcagcac agcgcgccag tggtaatcgc ctgagggcgc ggccgccggc
    10861 acgcgcaggg acgccaaagt gataacctgt tcccctgctt cccagtgcga tgttcgatag
    10921 ctctcgtttc cgaccggcag aacacttgag gaaaggacgt tgccggacga atcgaccagc
    10981 tcgatgcgga aggagcgatc ggtctgcggt tttcggtcgg cctgccagaa gaaggtgagc
    11041 aggatgctct cgccgggcac gccttcgacg cggtcaatgt tgtagccgag cagcgtcatg
    11101 tcgtcatcca gctcgtgacg ctcgccgccg tagatgcgca gttcgtcggc gtcgaaagat
    11161 cgcgttgcgc gatcgacgcg aatcttgccg atggaggcag cgacgcgccc gggcgccggc
    11221 agcgtgccgg tcagtagcgg cgcgagtgtc tcccggtcga agacctgcac gacgaggtca
    11281 tactcgccgg gcggtgcgcc aggcaggacc tcgacttcga agctgtcgac ggcatagcca
    11341 tcgagcggcc attcaggcat agatgggaag ctgttgtagt cgcgcggccg gtcggcgtgc
    11401 ttcgggctcc acaaccagcc atctgtatcg gcgatgctta cctcggcctg gtagttggct
    11461 gtgagcggct tgagagcttt ccagtacaga tcgacgcgga agcgctcgcc ggggcgggca
    11521 gtggacggcg tggccgtgta tccgagcaga cgtagctcgt cgccgaaggt cgcgtcgggt
    11581 gggtgggcga tgccgttcag tgcgtcgccg atcaagcgtg tggcgcgcag tggcgtcagg
    11641 ccgggatcga tcaatagact cttgacgacg acgtacacca aaagtgcgag caagatagat
    11701 aacgtgaagc gtgaggcgtg acgcgcaccg ctggcgataa gcaataagcg ataagcgata
    11761 agcgatcggc gagaccgcca atgaatcacc accgctgcta tcatggcgac caccgctatc
    11821 agcgatatgc catcggccag tgcccgctgc ggcgaatccc cgaattcgac gtggattcga
    11881 tgttctccgg caggcacgtc gaacgtcacc aggccgttcg gataggaggg ccggattgcg
    11941 atggggtgac cgtcgatttg tgcgttccag cccggaaagg cgaaggccag atacgttgcc
    12001 gtgaaggctg tcggcgacga aacgagaaca tcggcgcgat tgggaccata ttgtgcatcg
    12061 aacacccgcg cgccgccggg cagagctgac gcatcgaatc tttcgattgc ccggccggcg
    12121 ctgtacatgg cttcgagggg cgagccacct gggcgctcga tcaccgtgac cggcaggtac
    12181 gcgcccaatg ggtcgacgcc gacgtgcccg ctcaagcgct cgtaggcgaa tacgtcgttg
    12241 atgtccaggg cgtgtggcgt cgggcacacg cgcggatagg acagcggcgc cgtaaacaac
    12301 agggcgacca agacaaccgt tccgaacacg gtcacaacta accacgaagc ctgccctgag
    12361 cggagccgaa ggggcgcaaa gacacgaagt tggcttcgtg tcttcgtgtc tttgtggtta
    12421 attgagcgag gcggtgaaag agatgccgcg gccagaaggg cggtgggaag catagcgcgg
    12481 ccgacgaagc gccacgggaa ctggacgaat cgaatcaacg gcagcctttc ccacagcggc
    12541 agcgtgattg gcagagccat gagcgtgaat gtgatcgcgg cgactgcggc tgcgataacc
    12601 gtcgcgcgct gctcgcggtt ctgtgcgcgt cgatagccca gggcgccgag cgcggccacg
    12661 accaattgcg cccacccgaa cgagatgcgc agcggcgggt tgagcagatt aggatcgaac
    12721 gagccaggcc cgccgagcaa ctcctccagg ctgatgaaat tgaaatggta gtcgttgccg
    12781 cgtgtcgagt gcgtgaggta aagctgggct tcattctgct cggcgagcgc gggcagccag
    12841 aagaaggcgg tcaggcccaa agtaagagcg acggcgagta gggcgtatcg cgtatcgcat
    12901 atcgcatatc gcgtatcgcg tatggcgtat cgcacatggc ttatgataga cgacctatca
    12961 cgcggcgagg ctggactatc agctatcggc gatctgccat cagccatctg ccatctgcta
    13021 tctgctacct gcacccatcc cagtagcgca atgtacacca acaggaacgg cgtgaacagc
    13081 aagctcgata tgttgtgcgt gaggaacagt gcggcataac tggcggtcga tgcgacgaaa
    13141 taccgcgtat gcccatagac gatcaggcgg cggaagaaga acaagatgag cggcatcagc
    13201 gcgaaggcga tgacttcagg taaattgccg cgtacgagtg ggccaatgag cacatacggc
    13261 gcggccatgt gcaccacgcc ggccaacatc cccccgcggg gtccgaagat gtcgcgcccc
    13321 agcagatata tcgcgatgcc ggccacaacc acaccgccgg ccatcgtcac attgaatgcc
    13381 gttggcacgc tgaggcccaa aagatgtagc gcttcgagcg cgtagtaact gagcggctct
    13441 cggtacacga agaaggggaa gccgtaaccg taagccaggt cgggaaacca gcgcgggtac
    13501 agcaggccgt cgcggagggc gcgatcgagc gcgaccgtgc ggtagatgtg gagcgcgccg
    13561 tcgtcggtgc agagtgtctc gccctgcagg agaggcgcgg cgaggacgag gccgacggcg
    13621 atcaaaacga gaagggccgg cgctgagggc gggaggcggg ccataaggtg tgagcgtgtc
    13681 tgtcgataat cgactcgatg catggcgcga agcgtgcggg gcgcgcctcg attacggggc
    13741 gctcacctga agcgtgccga gcttaaggtg gcgatcaggc gcggcgggtc tattggcgtc
    13801 gcgcgcgggc aacgccgatc cgtcgagcga atcgtacatg ctgatagcga cgcggtacac
    13861 gccaggcggc gcgtccgaag gcagcgtcat tgtgtgtgtg tcctcgacga tctgatggcg
    13921 cacccagttc gtcgtcggcc acgcaccctg ctgcggccac gaatcggatt gcgctacggt
    13981 ctgcccgtcc ggggcgatca gacgcacgga aaccgagtag gcgacgagca cttcctcctc
    14041 agcacggcta gccagattca cctggagcga ctcgcccggt ttcaggtggg caggcagcgg
    14101 gcgcctcagg taaacgtcga acgttccgag cgagccgact gccaccgcgc caggcggcag
    14161 atcgaactca cgctcgacat ggctgatgtc gatgttcgtg gcgacgtcga gggtctcgcc
    14221 gtcgatcgac agtcgcaggt gataacgccc gtcgggcagg ccgagcggca ggcggacggc
    14281 gtagcgcgcg gcgacgatgt cgcccgcgcg caggcgcgca ggcgtgtgcg gcgcgagcgg
    14341 tgtctgccat tccagcgggg gcacatccgt cgtgaaggta ggctgcaacg accagcgcag
    14401 ttccattccc ggatccagcc catcgcgcag cgcgcgccaa tacattgtgg cgaagaagag
    14461 atcgcccgtc gatggcacgt aagagaggat gtcggcgccg atcagctcga cccggcctgg
    14521 ccaggcgtgc tcgatgcgtt gatgggtgag aatatccgtc gggttcgcga actgccggct
    14581 tggctcgatc gtgacgctgg cgaggacggg agcacttcca gtgaatgcgc ccgacgcgtc
    14641 gactagtcca gcgcgactgc cgtcggcgcg gtccacactg aagcgcaggc ggtacgatcc
    14701 cggcgccagc cagggcggaa cctgagcgga gaaggcctgc tcgaccacct cgccgggctt
    14761 ccactcggtt ggcgcgaagt cgctggcgtt gcgcagcagg cctccgatcc tcacccagcc
    14821 gtcgccgctg gcgtcgctca cctcgaaagt ggctgcgtaa tccgtcgccg gcggcgccag
    14881 cgtcagccag cgcgcagcca ccacgatgct atcgggatac gcggcggtat cggtcaacat
    14941 ctccagtccg agcagcgcca gcgagccatc gaactggacg acgaagggcg tgagagacgt
    15001 gctcgtctgg ttctctggcg cataaacgcg ggccgtcgct ccggggagcg gtgtctcact
    15061 ctccagccga gcgttcgcat cgagccaggc gcagacgtcg cggcgtacga cgggtgtcgc
    15121 cgccgcagtc gaaatgtaca ggatatgttc gtattcgtgc agcgcgtcca tcgtggacga
    15181 gatgccttcg ggcgtgacgt tcggcggcag gatacgggcc ggaagcggat cgagcaggtg
    15241 agccagcggc gtgggcgcgt cgagcaggat cgcgtcgcgc ggcccggcgc ggcggcgtat
    15301 ccaggccgcc tgctcggcgt ggcgatcgtt gcggtacacc cacgtcgtgt cgatgccgcc
    15361 gctgcggatt gtatgcacga gcgtgccgcg ctgcgccagc gaggcaaaga aatcgggcgc
    15421 ctgttgaact tcgctgatgg tgaagatgat gtggtcggca gcagccaagc ccgcgtcgtt
    15481 caaggtggcg atccgtccgg cgtacagcgg cacgagattc ggcaggccgc cgatggcgac
    15541 ggttgcgccc ggctggcgct cggcgaccca gcgcgcggcg gcgatcagac cctcgcccca
    15601 gccgacggcg atgcgatcgc gcgcgccggc tgcgccaccg agcagcggat tgtaggccct
    15661 gagcgggtag gggatgacgc ttaacagcaa aatggcctgg aggaggatgg ccgccgggga
    15721 caggagccgg gaaagtctgc tggtgggttt gtcggtgagt tggtggatgc cccaggcggc
    15781 ggcgatgatg agcggcggga tggcgggcag cacatagcga tcaaacttct tggcggcggg
    15841 cgtgagaaag acgatgaagc tcatggcgaa aaggagta
//
LOCUS       DBNHXB010000228         5612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2233633, whole genome shotgun sequence.
ACCESSION   DBNHXB010000228 DBNHXB010000000
VERSION     DBNHXB010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2233633"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="AC162G_09415"
     CDS             complement(<1..292)
                     /locus_tag="AC162G_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR5076074.1"
                     /translation="MATHSITLHPSDKTVSVPTGALVIEALREAGLDVAQPCGGQGRC
                     GRCAVVVESGAVRRRSTIRLSQADIEAGYALACQTIIEGDAAILVPEQEKVER"
     gene            complement(402..620)
                     /locus_tag="AC162G_09420"
     CDS             complement(402..620)
                     /locus_tag="AC162G_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076075.1"
                     /translation="MISLQITLADLVAGASEERCLGCPPDRVCAWACWQGMGVADNAI
                     AEMLGFPLDEPLDADDEFVRQALCECYT"
     gene            complement(659..2146)
                     /locus_tag="AC162G_09425"
     CDS             complement(659..2146)
                     /locus_tag="AC162G_09425"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5076076.1"
                     /translation="MHEHPERIAGRKERLQIPHTPYLERLPVERIRDFDDIVVGYTAE
                     EAMLEASRCLQCPQPAACAEACPLHNDIPGALWLIAQGDFVGAANLYRQTSIFPEICG
                     RVCPQEKLCEGACTTGKHHTAPSLGKLEMFVADYQRQIEGWPRPPMEQPTGHKVAVVG
                     SGPAGLAVAETLIQRGHGVTIYEAMPYPGGLLMYGIPNFKLPKSLIQEKIEFLERLGV
                     KFVCNARIGEALAVDDLFEAGFEAVFVGVGANVDAPLKAPGVDLRGVYQAGEFLIRSR
                     SGMFHSRSPQDIVPPALRDTPRVGKRVAVIGGGDTATDCMRTALRLSAEAVVCYYRRT
                     EAEMPGSKKERGHALEEGARVEYLVAPVKFFGDEARRLRAMELQRMELGEPDKSGRRR
                     PVPIEGSEFTVPVDTVVLALGYWPDETIGKTTPGLETHDYGLISADGASGRTSRRGVF
                     AGGDAVTGPDLVSTAVAAGLRAARAIDAYLAAGETRVRVEGPEAV"
     gene            complement(2158..3498)
                     /gene="acsC"
                     /locus_tag="AC162G_09430"
     CDS             complement(2158..3498)
                     /gene="acsC"
                     /locus_tag="AC162G_09430"
                     /inference="COORDINATES: protein motif:HMM:NF003195.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HIR5076077.1"
                     /translation="MALTGLQIYKLLPQTNCKECGYPTCLAFAMKLAAKQAELAACPY
                     VTEESKAQLEAAAAPPIRLVTVSSDSAKIEAGNETVLFRHEKTFYHKPGLFIRVKDSE
                     GAEAIEAKVAAADAYTVDYVGIKLGVDGFAVEAASGDGAKFAEAVGAVRRASKKPLIL
                     IAGDAGAMADGAKAAGGTRPLLHAADESNWQKMVEVAKMAKAALAVKAATLEALAGLT
                     EDIKKAGFEDLVLDPAARDYGGSLTTLTQIRRLALKKNFRALGYPVIAFPGEANGVDE
                     TVLAAQHIAKYAGFVVLESFDPATLYPLLVLRQNIYTDPQKPIQVQPGLYEINSPKAD
                     SPLMVTTNFSITYFAVNNEVEGSGWPAWLVVADAEGMSVLTAWAAGKFDADRIAKAVK
                     TFGVEGKIDHKRLIIPGQVAVLSGELEESLPGWQIMVGPKEAVDIPGYLKVLWK"
     gene            complement(3561..3842)
                     /locus_tag="AC162G_09435"
     CDS             complement(3561..3842)
                     /locus_tag="AC162G_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013286.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR5076078.1"
                     /translation="MTTLFAGAQPETNGVPAKGTPEERMRALIETLSAYIEAYHGGAV
                     EMVSFDGERLTIRMSGACDGCPLSAVTLHGWVEGTVRQFFPELKKVEAV"
     gene            complement(3857..4828)
                     /locus_tag="AC162G_09440"
     CDS             complement(3857..4828)
                     /locus_tag="AC162G_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HIR5076079.1"
                     /translation="MPVTVEIPKEKWVGKVREVTLGATAADGGTRARTVTVGGESTLP
                     FLHFDGAIPHRPALAIEVRDRRPDDWSPLLYDAWGDAMNDPGEWAKAAEAAGADLIVL
                     TLSLTKADGSPNTPDHARAVVRKVLNSTGLPLAALGPGQAEKDNELIVPVADEAKGER
                     LLIGVCEDKNYRTIVAAAMANDHLVSSKTPMDVNLAKQLVILIHDMGMPLERVIMDPT
                     TGALGYGIEYGYSVMERLRLAALQGDGMTQQPMIVTPGEETWKAKESKVGEGVPEAWG
                     EWKRRAINWETLTASSLIHAGANIVVLRHPESLARVRSMVNGLMGSN"
     gene            complement(5028..5402)
                     /locus_tag="AC162G_09445"
     CDS             complement(5028..5402)
                     /locus_tag="AC162G_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5076080.1"
                     /translation="MKSLLVRDVMTIGVPVCRDTERCGDVEARLARQAVQVEVVGVLD
                     EDGSACGWVSRARLAENAARTVGEVMEEDIQTVPPDIPAEAAAQIMRDRGVAYLFLMH
                     DWPGEPRPSAVISLKAIEQRIG"
BASE COUNT          942 a         1996 c         1610 g         1064 t
ORIGIN      
        1 ggcgctcgac cttctcttgc tcagggacga gtatcgccgc gtcaccttcg atgatcgtct
       61 ggcaggcgag cgcgtagccg gcctcgatgt ccgcctgcga caggcggatc gtcgagcgcc
      121 gccggaccgc gccgctctcg acgacgaccg cgcagcgccc gcagcgtccc tgcccgccgc
      181 acggctgcgc gacgtccaga ccggcttcgc gcaaggcttc gattaccagc gccccggtcg
      241 ggacggagac ggtcttatcg gaaggatgta gcgttattga gtgagttgcc attcgggttc
      301 aaaatccaaa tcccaaactc caaacttcaa acctccaacc tccaacttcc aatctccaac
      361 ctccaatctc caatctccaa cctccaatca ccaattacca attacgtata acactcacac
      421 agcgcctgtc taacgaactc gtcgtccgca tcgagcggct catcgagcgg gaagccgagc
      481 atctcagcga tggcattgtc ggcgacgccc atgccctgcc agcacgccca ggcgcacacg
      541 cgatcgggcg ggcagcccag gcagcgctcc tcactcgccc cggcgacgag gtccgcgaga
      601 gtgatctgca agcttatcat gataaactct ttagcgcttt cgcccaccgc cgtcgcgtct
      661 agaccgcctc cggtccttcc acgcgcacgc gcgtctcccc tgcagccaga tatgcgtcga
      721 tcgcgcgcgc cgcgcgcagc ccggccgcca cggccgtgct caccaggtca ggcccggtca
      781 ctgcgtcacc cccggcaaac acgccacgcc ggctggtgcg tccgctcgct ccgtcggcgc
      841 tgatcaaacc atagtcgtgg gtctccagac cgggcgtggt tttgccgatt gtctcgtcag
      901 gccagtagcc gagcgccagc acgacggtgt cgaccgggac cgtgaactcc gatccctcga
      961 tcggcaccgg ccgccgccgc ccggatttgt ccggctcacc aagctccatg cgctgcagct
     1021 ccatcgcacg caagcgccgg gcttcgtcgc cgaagaactt caccggggcg acgaggtact
     1081 cgacgcgcgc gccctcttcg agcgcgtgcc cgcgctcttt cttggagccg ggcatttcgg
     1141 cctcggtgcg gcggtaatag cacaccactg cctccgcgct cagccgcagc gcggtgcgca
     1201 tgcagtccgt cgccgtatcg ccgccgccga tgacggcgac gcgcttgccg acgcgcggcg
     1261 tatcgcgcaa cgcaggtggc acgatgtctt ggggcgagcg gctgtggaac atccccgacc
     1321 gggagcgaat gagaaactcg cccgcctgat acacgcccct gagatcgacg cctggcgctt
     1381 tgagcggggc gtcgacgttt gcgccgacgc cgacgaacac cgcctcgaat ccggcctcga
     1441 acagatcgtc caccgccagc gcctcgccaa tgcgtgcgtt acagacgaac ttcactccca
     1501 ggcgctcgag gaactcgatc ttctcctgaa tcagcgattt gggcaacttg aaattgggaa
     1561 tgccatacat caggagaccg cccggatagg gcatcgcctc gtatatcgtg acgccatggc
     1621 cgcgctgtat caaggtctcg gcgacggcca gtccggccgg ccccgatccg acgaccgcca
     1681 ctttgtggcc ggtaggctgc tccatcggcg gtctcggcca gccttcgatc tgcctctggt
     1741 aatcggccac gaacatttcc agcttgccga gcgatggcgc cgtgtgatgt ttccctgtgg
     1801 tgcacgctcc ctcacacaac ttctcttgcg gacacacgcg cccgcagatt tcggggaaaa
     1861 tgctggtctg ccgatacaga ttcgctgcgc cgacgaagtc gccctgcgcg atcagccaga
     1921 gcgcgccggg gatgtcgttg tgcagcgggc acgcttcggc gcacgccgcc ggctgcgggc
     1981 attgcaggca gcggctcgct tccagcatcg cctcctcggc cgtgtagccg acgacgatgt
     2041 catcgaaatc gcggatgcgc tccacaggca ggcgctccag gtacggcgtg tgaggaattt
     2101 gaagacgctc tttgcgcccg gcaatgcgct caggatgttc gtgcacgttc ttcctcccta
     2161 tttccacagg accttgagat agcccggaat gtcgacggcc tccttcgggc cgaccataat
     2221 ctgccagccg ggcaggcttt cctcaagctc acccgacagc accgcaacct ggcccggaat
     2281 gatcagacgc ttgtggtcga tcttgccctc cacgccgaac gtcttcaccg ccttggcgat
     2341 tcgatcggcg tcgaacttgc cggcggccca cgcagtgagc acgctcatgc cttcggcgtc
     2401 cgcgaccacc agccaggccg gccagccgga gccttccacc tcgttgttca ccgcgaaata
     2461 cgtgatcgag aaattggtcg tcaccatcaa aggcgaatcg gctttcggcg aattgatctc
     2521 gtacaacccg ggctgcacct ggatcggctt ctgcgggtcg gtgtagatgt tctggcgcag
     2581 aacgagcagc gggtacagcg tcgccggatc gaacgattcc agcacgacga atccggcgta
     2641 cttggcgatg tgctgcgcgg ccagcaccgt ctcgtccacg ccattcgctt cgccggggaa
     2701 ggcaatcacc ggatagccga gcgcccggaa gttcttcttg agcgccagcc tccgaatttg
     2761 ggtaagggtc gtcaaagaac caccgtagtc gcgggccgcc ggatcgagca ccaggtcttc
     2821 gaagcccgcc ttcttgatgt cctcggtcag cccggccagc gcttcgagcg tggccgcctt
     2881 cacggccagc gccgccttcg ccatcttcgc cacctcgacc atcttctgcc agttggattc
     2941 gtcggcggcg tgcagcagcg gccgggttcc gccggcggcc ttcgcgccgt cggccattgc
     3001 acccgcgtcg cccgcgatca ggatgagcgg cttcttgctc gcccggcgca ccgcgccaac
     3061 agcctccgcg aacttcgccc cgtcgcccga cgcggcctcg acggcgaagc cgtccacgcc
     3121 cagcttgatg ccgacgtagt cgaccgtgta cgcgtcggct gctgcgacct tcgcttcgat
     3181 cgcctctgcc ccctcgctat ccttcacgcg aatgaagagg ccgggcttgt gatagaaggt
     3241 cttctcgtgg cggaacagta ccgtctcgtt gcccgcctcg atcttcgcgc tatcgctgga
     3301 gaccgtgacc aggcggatcg gcggcgccgc ggcggcctcg agctgcgcct tcgactcctc
     3361 ggtgacgtac gggcaggcgg ccagctcggc ctgcttggca gctagcttca tcgcaaacgc
     3421 caggcaggtg ggatagccac attccttgca gttggtctgt gggaggagct tgtaaatctg
     3481 tagtcctgtc aatgccatgg tcaatctccc tgttcagtgc caggtaccac gtggcacttg
     3541 gcactccgca cttggcgctc tcacaccgct tccactttct tgagttcggg aaagaactgc
     3601 ctgaccgtgc cttctaccca gccgtgcagc gtaaccgccg acagcgggca tccgtcgcac
     3661 gcgccggaca ttctgatcgt cagtcgctcg ccgtcgaacg acaccatctc gaccgcgccg
     3721 ccgtgatacg cctcgatgta tgcgctcagc gtttcgatca gcgcgcgcat gcgctcctcg
     3781 ggtgtccctt tggcgggcac gccattcgtt tcgggctgtg cgccggcgaa cagagtggtc
     3841 atcgcgctcc ttcccgtcag ttcgatccca tcaatccgtt caccatcgag cggacgcgcg
     3901 ccagcgactc aggatggcgc agcacgacga tgttcgcgcc ggcgtgaatg agcgaggacg
     3961 ccgtcagcgt ctcccagttg atcgcgcgcc gcttccactc gccccacgct tccggcacgc
     4021 cctcgccgac cttcgactct ttcgccttcc acgtttcttc gccgggcgtg acgatcatcg
     4081 gctgctgggt catgccgtcg ccctgcagcg ccgccaggcg caggcgctcc atgaccgagt
     4141 agccatactc gatcccgtag cccagcgcgc ctgtcgtggg atccatgatg acgcgctcca
     4201 gcggcatgcc catgtcgtgg atcaggatga cgagctgctt cgccaggttc acgtccatcg
     4261 gcgtcttgga gctgaccaag tgatcgttcg ccatcgccgc cgcgacgatc gtgcggtaat
     4321 tcttgtcctc gcacacgccg atcagcagcc gctcgccctt cgcttcgtcc gcgacgggca
     4381 cgatcagctc gttgtccttc tccgcctggc ccggcccgag ggcagccagc ggcaggccgg
     4441 tcgagttgag gaccttgcgg acgacggcgc gcgcatggtc cggcgtgttc gggctgccgt
     4501 ctgccttcgt gagcgacagc gtcagaacga tcaggtcggc gccggccgct tcagccgcct
     4561 tcgcccactc gcccggatcg ttcatcgcat cgccccacgc gtcgtagagc agcggcgacc
     4621 aatcgtccgg cctgcgatcc ctgacctcga tcgccagcgc cggccggtgc gggatcgcgc
     4681 cgtcgaagtg caggaacggc agcgtgctct cgccgccgac cgtgaccgtc ctggcgcgcg
     4741 tgccgccgtc cgccgccgtt gcgccgagcg tgacctcgcg gaccttcccg acccattttt
     4801 ctttaggaat ttcaaccgta accggcatca acgcctccct cgtcaattga gatgaacccg
     4861 aaccacttca cgagcgaaat tgacaggaac ttcgtatcgc cttccactca ctttaactct
     4921 aactctaact cctcactcct gttgtaccat ccgcccatgc tcatcacccc tgccccatcc
     4981 gctgaattca cccgatccgc tgctccctgt ccatacatcc gctgaattca cccgatccgc
     5041 tgctctatgg ctttcagcga gatgaccgcc gacgggcgcg gctcgccggg ccagtcgtgc
     5101 atcagaaaga ggtacgccac gccgcgatcg cgcatgatct gcgccgccgc ctcggccgga
     5161 atgtcgggcg gcacggtctg tatgtcttct tccatcactt cgccaaccgt gcgagccgcg
     5221 ttttccgcga gtcgggcgcg cgacacccag ccgcacgccg aaccgtcttc gtccagcacg
     5281 ccgacgactt caacttgaac ggcttggcgc gccagacgcg cttcgacgtc gccgcatctc
     5341 tccgtgtcgc ggcagactgg aacgccgatg gtcattacgt cgcgcacgag taaagatttc
     5401 atttcagctc gtcgcttcag gctcgtatcc ggcatagttg acagctcaat ccgacaaggc
     5461 tacaatcagg tttatgaggt gcgccatggg catcgatgaa ctgattcgca gtatccacac
     5521 actcgaaaac catcttcaga agttcgaaga gaagtaccaa cttcgctctg aagacttcta
     5581 tcggctcgtc tcggaaggca aactcgaaca ga
//
LOCUS       DBNHXB010000229         4213 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_723921, whole genome shotgun sequence.
ACCESSION   DBNHXB010000229 DBNHXB010000000
VERSION     DBNHXB010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_723921"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="AC162G_09450"
     CDS             complement(<1..340)
                     /locus_tag="AC162G_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5076081.1"
                     /translation="MLYQSYAYGKNHWLLDPDLKPILSRYWKDLSDHEQALIDFGALA
                     GGRAYEVADHVDHGAAPILVMHDLDGQRVDRARLSPAHADLLKHMAFINRPPYEGGSW
                     HHHFAFGYLLA"
     gene            complement(425..697)
                     /locus_tag="AC162G_09455"
     CDS             complement(425..697)
                     /locus_tag="AC162G_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076082.1"
                     /translation="MNATIDWLLEKENPSDVLEASLALVEAGYGRDPRLKNAIELILS
                     KRDADGRWAMKHSLNGKMWADVDVKGRPSKWVTLRALRVLKAAGVD"
     gene            complement(779..1984)
                     /locus_tag="AC162G_09460"
     CDS             complement(779..1984)
                     /locus_tag="AC162G_09460"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR5076083.1"
                     /translation="MSNIILEELKQTPVEQQRVELVERKGTGHPDSICDAIMEQISVA
                     LCREYLDRFGRILHHNIDKGLLVAGRADPRPGGGSVMEPMRLVIGDRATAAYKGKRID
                     VGAIAEAGARDWVRQNLRFVEPEKHIVFQNELKEGSPELTDIFERDVISANDTSAAVG
                     YAPLTETERIVFATERYLNSKEFKQHFPEAGEDVKVMGYRRDRELVLTVALAFVDRFV
                     PDAKTYFARKQEIQVALEEYLAREQRTLDRTTVTINTLDDPARGEGGMYLTVLGTSAE
                     GGDCGQVGRGNKVNGVIALNRPMSTEAAAGKNPVSHVGKIYSLLTHRMAAEIYASVPG
                     LREVYVWLCSQIGKPIDQPLIASAQLILQQGVALADVKPAVESVVEHELATVGDFTAR
                     LARGELPVW"
     gene            complement(2050..2670)
                     /locus_tag="AC162G_09465"
     CDS             complement(2050..2670)
                     /locus_tag="AC162G_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076084.1"
                     /translation="MTSILNKLSSQVADRTETANKRVAAQALKRPTRLAEIAAGLASA
                     DAKLAGDCAEVMTLVAAERPDLVSPHAGALIAILDRPDTRVRWEAMHSLAEIAAHVPN
                     RLSPIVPKLAEKIALDKSVIVRDYAIETLGEYGRTSASAACRAWPHLREALTLWEGRH
                     AGKVLEAMRKLVAMDTALKAEAQDIAGRFVDHSSAKVRTAAKRLLR"
     gene            complement(2687..3712)
                     /gene="amrS"
                     /locus_tag="AC162G_09470"
     CDS             complement(2687..3712)
                     /gene="amrS"
                     /locus_tag="AC162G_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HIR5076085.1"
                     /translation="MIDSMFVRESLLQERTDGKVRCHVCERRCTIGAGGAGWCRTREN
                     RSGRLVTLIYGAASSIAVNPIEKKPFYHFYPGTLTLTAGSWSCNFGCPWCQNWDISKT
                     PPPRAGEYVSPERFVELAENYTCQGTSISFNEPTLSLEWSLDVFRLARGRGLYNTYVT
                     NGYMTPEALSLLIDAGLDAMNVDVKGDAVAVRKYGKGIDVDKVWAVCRLARSGSVHIE
                     ITTLVIPTVNDSDAALRSIAERMVNDLGPDVPWHVTGYYPAYKFTAPPTSIRTLERAW
                     HIGKDAGLEFVYVGNVPGHRFDNTYCPTCDTLLLRRRGFDVLHNAVRRGRCPHCERPI
                     AGVWGEE"
     gene            complement(3726..4142)
                     /locus_tag="AC162G_09475"
     CDS             complement(3726..4142)
                     /locus_tag="AC162G_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076086.1"
                     /translation="MSISGTGSCSLCEGVSLPVSSALALCAHCLRERFAEVKSRVQAV
                     HLASRAEFGLPGEPPRHSNGIRCTLCANECMIGEGERGYCGLRTVRHGRLNHLAGTPA
                     RGRPRQRAGRESAPAGVGASVTAARDFAGVNHAAYV"
BASE COUNT          701 a         1416 c         1261 g          835 t
ORIGIN      
        1 ccgccagcaa gtagccgaac gcgaagtgat ggtgccacga gccgccttcg tagggcgggc
       61 gattgatgaa agccatgtgc ttgagcaggt cggcgtgggc cgggctgagc cgcgcccgat
      121 ccacgcgttg gccgtccaga tcgtgcatca cgagtatcgg cgccgcgccg tgatcgacgt
      181 ggtcggccac ctcgtaggcc cgtccaccgg cgagcgcgcc gaagtcgatc agcgcctgtt
      241 cgtgatcgga caaatccttc cagtagcggc tcaggatcgg cttcaagtcc ggatcgagga
      301 gccagtggtt cttaccatag gcgtaggatt gatagagcat tgctcttccc ttccgctcgt
      361 cgagggtctg tgaccctcag cctcccggcc gccgggcgac acacccggcg ggagcagcca
      421 taaatcaatc gacacccgcg gccttgagca cccgcaacgc gcgcagcgtc acccacttgc
      481 tgggcctgcc tttcacgtcg acatccgccc acatcttgcc gttgaggctg tgcttcattg
      541 cccatcgacc atccgcgtct cgttttgaga gtatgagctc gatcgcgttc ttgaggcgcg
      601 gatcacgccc gtaacccgcc tcgaccagcg cgagggacgc ttcgaggaca tccgagggat
      661 tttctttctc taacaaccag tcaatcgtcg cgttcattga gcctctcctg gctcactgtg
      721 tgccaacgtg tcgaagttag gtgaatctgg ttcgctgaat gcgagggtgg atcttccact
      781 accacaccgg cagctcaccc cgcgccagcc gcgccgtgaa gtcgccaacc gtcgccagct
      841 cgtgctcgac gaccgattcg acagccggct tgacatccgc cagcgcgacg ccctgctgca
      901 ggatcaactg cgccgacgca atcaacggct ggtcgatcgg ttttccgatc tggctgcata
      961 gccacacgta gacctcgcgc agcccgggca cgctggcgta aatctcggct gccatacgat
     1021 gtgtcagcag cgagtagatc ttgccgacgt gactcaccgg gttcttgccc gccgccgctt
     1081 cggtgctcat cggacgattg agcgcgatga cgccgttcac cttgttgccg cgcccgacct
     1141 gcccgcagtc gccgccttcg gccgaggtgc ccagcacggt caggtacatg ccgccttcgc
     1201 cgcgcgccgg gtcgtcgagc gtgttgatcg tcaccgtggt gcgatccagc gttcgctgct
     1261 cgcgcgccag atattcttcc agcgcgacct gaatctcctg cttgcgcgcg aagtacgtct
     1321 tcgcatccgg cacgaaccga tcgacgaagg ccagggcgac ggtcaataca agctcgcggt
     1381 cgcgccggta gcccatgact ttcacgtctt cgccggcttc ggggaaatgc tgcttgaact
     1441 ccttagagtt caggtagcgt tccgtggcga acacgatgcg ctcggtttcg gtgagcggcg
     1501 catagccgac cgcggccgac gtgtcgttcg cgctgatcac gtcgcgctcg aagatgtcgg
     1561 tcagctcggg cgagccttct ttcagctcgt tctggaacac gatgtgcttc tcaggctcca
     1621 caaagcgcag gttctgcctt acccaatccc tcgcgccggc ctcggcgata gcaccgacgt
     1681 cgatgcgctt gcctttgtac gcagcggtgg cgcggtcgcc aatcacgaga cgcatcggct
     1741 ccatcacgct gccgccgccc ggccggggat ctgcccgccc ggcgacgagc agccccttgt
     1801 cgatattgtg gtgaaggatg cgaccgaacc tgtccaggta ttcccggcac aacgcgaccg
     1861 agatttgttc catgattgcg tcgcagatcg agtcgggatg gcccgtgccc ttgcgctcca
     1921 ccagctcgac ccgctgctgc tcgaccgggg tctgcttcag ctcttcgagg ataatgttcg
     1981 acatgcgcgt cgctcctttg atgggcggcc gcaggggtcg ccctaagtta tgcgcctgcc
     2041 agggtccgat cacctgagaa ggcgtttggc tgccgtgcgc accttcgcgc tgctgtgatc
     2101 cacgaatctt cctgcaatgt cttgcgcttc cgccttgagc gctgtgtcca tcgcgaccag
     2161 tttccgcatc gcctcgagca ccttgccggc atgcctgcct tcccagagcg tgagcgcctc
     2221 gcgtaaatgt ggccaggctc tgcacgccgc cgaggcgctc gtgcgcccgt attctcccag
     2281 tgtttcgatc gcgtaatccc gtacgatgac gctcttgtcc agcgcgattt tctcggccag
     2341 cttcggcacg atcggcgata gtctgttggg gacgtgcgcg gcaatctccg cgaggctatg
     2401 catggcttcc catcgtacgc gcgtgtccgg gcgatccaga atcgcgatca gcgcgccggc
     2461 gtgcggcgag acgagatcgg gtctctctgc cgcgaccagc gtcatcacct cggcgcaatc
     2521 gcccgcgagt ttcgcgtccg ccgacgcaag cccggcggcg atctcggcca accgggtcgg
     2581 ccgtttcagc gcctgtgcgg ctacccgctt gttcgccgtt tccgttcggt cggcgacctg
     2641 tgagctgagt ttgttcagga tgctcgtcat cttccctcta gtttgcctat tcctcgcccc
     2701 acacgccggc gatgggccgc tcgcagtgcg gacaccgccc gcggcgcacc gcgttatgca
     2761 gcacgtcgaa ccctctgcgg cgaagcaata gcgtatcaca cgtggggcaa tatgtgttat
     2821 caaagcgatg gccgggcaca ttgccgacgt agacgaattc caatccggca tctttgccga
     2881 tatgccaggc gcgttctaaa gtgcgaatcg acgtcggagg agcggtgaac ttgtaagccg
     2941 ggtagtagcc ggtgacgtgc cacggcacat ccggtcccag atcgttcacc atgcgttccg
     3001 caatgctgcg cagcgcggcg tccgagtcat tcacagttgg aatcacgagc gtcgtgattt
     3061 cgatgtgcac gctgccggag cgtgccagcc tgcagacggc ccacaccttg tccacgtcga
     3121 tgcctttgcc gtacttcctc accgccacgg catcaccctt cacgtcgacg ttcatcgcgt
     3181 ccagcccggc gtcgatcaag agcgacagcg cctccggcgt catgtagccg ttcgtcacgt
     3241 acgtgttgta caggccgcgc ccgcgcgcca ggcgaaatac gtccaacgac cactccagcg
     3301 acagggtcgg ctcattgaac gagatcgacg tgccctggca tgtgtagttc tccgcgagtt
     3361 ccacgaatcg ttctggcgag acatactcac ctgcacgcgg cggcggcgtt ttgctgatgt
     3421 cccagttctg gcaccatggg cagccaaagt tgcacgacca actgccggcg gttagtgtaa
     3481 gcgtgccggg gtaaaaatgg tagaacggct ttttttcgat cgggttgact gcgatagacg
     3541 aggccgcccc gtaaatcagg gtgaccagtc gtccggagcg gttctcgcgc gtgcgacacc
     3601 agcctgcgcc gccggcgccg atcgtgcagc gccgctcgca gacgtgacag cggactttgc
     3661 cgtcagtcct ctcttgcaag agcgattcgc gcacgaacat gctgtctatc atctgcgcct
     3721 cgtcatcaga cgtaggctgc gtgattcact ccggcgaagt cccgcgccgc cgtcacgctt
     3781 gctcccaccc cagcaggtgc cgattcccga cccgcacgtt gacgaggccg gccgcgcgcg
     3841 ggcgtgccgg cgagatggtt gagcctgccg tggcgcacgg tgcgcaatcc gcaatagccg
     3901 cgctcgcctt cgccgatcat acactcgttg gcgcacagcg tgcagcggat gccgttggaa
     3961 tgtcgtggcg gttcgcccgg cagaccgaac tcggcgcgcg acgcgaggtg gacggcctgc
     4021 acacgcgact ttacctccgc aaatcgctcg cgcagacaat gagcgcagag agcgagtgcg
     4081 gacgacaccg gcagcgagac gccttcgcac agcgagcagc ttccggttcc cgagatgctc
     4141 atcgctcaac acgtgacgtg gaacaagccg atcacgccct ccacctcgcc gctcacatca
     4201 catgctcccc tgg
//
LOCUS       DBNHXB010000230         5497 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_53851, whole genome shotgun sequence.
ACCESSION   DBNHXB010000230 DBNHXB010000000
VERSION     DBNHXB010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_53851"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2115
                     /locus_tag="AC162G_09480"
     CDS             <1..2115
                     /locus_tag="AC162G_09480"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR5076087.1"
                     /translation="VTVRQGDTVILATATMAGQPREGIDFFPLSVDYEERLYAGGRIP
                     GSFFRREGRPSEAAILIGRLVDRPLRPLFPKDLHNDVQVIVTSLSSDAEHPIDILAIN
                     GASAAMMISDVPFYGPVGAVRVGRINAELVINPTYGQMEQSDLDLRIAGTADAILMVE
                     CGANEVPEDVMVQALLKGHQALQSIIAVQNEMRAQVGKPKNESYPRFGIADDVRSRVT
                     ERINRRIRDILINLYDKDARNEALAELEEELVTEFAEYFDARHVKEAFHDRLKIEVRE
                     RILSDGIRPDGRGPTDIRPLAAEVDLLPRVHGSGLFTRGETQVLSAATLGTARDAQEL
                     DGLAPVDEKRYMHHYNFPPFSTGEARPLRGQSRREVGHGALAERALAAMLPSMEEFPY
                     TIRVVSEVLSSNGSTSMASVCGSTLSLMDAGVPIKAPVAGIAMGLVSDGERYKILTDI
                     QGMEDHLGDMDFKVAGTAKGITALQMDSKIKGLTAHLLKEALEQARAARLQVLDVMRS
                     AIAEPRPELKPHTPRILTIKINPDKIGAVIGPGGKMVRSIQDETGATIDIQEDGTIYI
                     AATGGTSADAARARIELLTESPELGRIYSGKVVRTTDFGAFVEIMPGVDGMVHISQLA
                     DYRAPNVESVVKVGDEVMVMVTDIDVNGKIRLSRKAVLEGMTVDEARESDVRRGGAGR
                     SGRGGAGRSPRREGGDERRRRR"
     gene            2231..3331
                     /locus_tag="AC162G_09485"
     CDS             2231..3331
                     /locus_tag="AC162G_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR5076088.1"
                     /translation="MTPASPSWSTDTKRLVALFAVILVGLTIYRFNFIVGPLAISLLI
                     TYLFAPVVGWIERRLHGRRAIATLIVLLTLVMVLVLLAALLIQTVIVQVRSFNLDLDA
                     IYERISERLAEPLVVGDVRLDLRAAFEPLRGSLSSLLQTVVNRIVDVGANLAEGFIWL
                     VFIFISTFYLLKDWRAIGRWFENTTPPGFRGDFIYLRGQIGQTWNNFFRGQLVLALVM
                     GTLVGLAVWVIGLPNAFVIGVLFGVLEVVPNFGPVIASIPTVLIALFQGSSWMFVGNN
                     LAFTVVVIILLFLLQQTENAILVPRILGHHLNLHPVAVLVAAIAGASLAGVLGILLAS
                     PVLATLRDIARYVGARMLDLDLYSSGSSPPGV"
     gene            3587..4453
                     /locus_tag="AC162G_09490"
     CDS             3587..4453
                     /locus_tag="AC162G_09490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5076089.1"
                     /translation="MSGEFVFRPVRSEDKSRVLEFTAHTWGDGDYIHDVFDLWLADTD
                     GQFTAVELDGRAVAIAKLTDLGEGEVWLEGLRVDPAHRRKGIGQALHNYELGLARQIG
                     ARILRYATGTKNTVSLMFGERTGFRRVCDFRWHVADASTEFSPPEPLRPDDLPQLRAW
                     LDSPLLRSAHGLYPQLWKWSRLSEARLVRHLRAGEVFGLPGASGLRAWSICSLFEGWD
                     EAQMHHLDGADPASIVELAQSMRGYAAGGGREKIECFALDPSPLIDLLRDAGYAGEDF
                     AVTVLELELERA"
     gene            4450..4749
                     /locus_tag="AC162G_09495"
     CDS             4450..4749
                     /locus_tag="AC162G_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076090.1"
                     /translation="MTTVREALHRLFRRPEPVGTAGLDRAGYSYRVFWMKQARGWDAA
                     RRQSVLEATRVVTRADAFESNTFERRYQVQGLAGEHSGASLLALIQVLEALNAGE"
     gene            4736..4936
                     /locus_tag="AC162G_09500"
     CDS             4736..4936
                     /locus_tag="AC162G_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076091.1"
                     /translation="MPANESDSNAREALKQRMIQVLREPLYVAIEREFSRMLLRENIV
                     LSRAEYRKLKWEVVRAIVNSGE"
     gene            4949..>5497
                     /locus_tag="AC162G_09505"
     CDS             4949..>5497
                     /locus_tag="AC162G_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HIR5076092.1"
                     /translation="MPTMKCPNCQHDNPPEATFCMNCGTKLSRTCATCGATLPAGARF
                     CMSCGQPAGGSTPADEARLARLAAAAPVPLAEKMRAAHLEGERKQVTVLFADVVGSTA
                     LAERMDPEEWTTIMNRAFEQLSPAIYRYEGTIARLMGDALLAFFGAPLAHEDDPMRAL
                     RAALDLLKAARAYGDELRRERGI"
BASE COUNT          940 a         1702 c         1829 g         1026 t
ORIGIN      
        1 gtgaccgtgc gtcagggtga cacggtcatt ctggccacgg cgacgatggc cgggcagcca
       61 cgcgagggga tcgacttctt cccgctcagc gtggactacg aagagcgtct atatgccggc
      121 ggccgtattc ccggctcgtt cttccggcgt gaaggcaggc cgtccgaggc ggccatcctg
      181 atcggtcgcc tggtcgaccg acccctgcgg ccgctgttcc ccaaggacct gcacaacgac
      241 gtgcaggtga tcgtcaccag tctcagctcg gacgccgagc acccgataga cattctggcg
      301 atcaacggcg cgtcggcggc gatgatgatc agcgacgtgc ccttttacgg ccctgtgggc
      361 gcggtgcgcg tcggccggat caacgccgag ttggtcatca acccgacgta cgggcaaatg
      421 gaacaaagcg atctggatct gcggatcgcg ggcacggccg atgcgatcct gatggtcgag
      481 tgcggcgcga acgaagtgcc cgaggacgtg atggtgcagg cgctgctcaa gggtcaccag
      541 gcgctgcaat ccataatcgc cgtccagaac gagatgcgcg cccaggttgg caagccgaag
      601 aatgagtcgt atccgcgctt cggcatcgcc gacgatgtca gaagccgtgt gactgagcgg
      661 ataaaccgcc gcatccgcga catcctgatc aacttgtacg acaaagatgc acgcaacgag
      721 gcgctggccg agctcgaaga agaactggtg accgagtttg ccgagtactt cgacgcccgg
      781 cacgtcaaag aggcgttcca cgatcggctc aagatcgagg tgcgcgagcg catcctgagc
      841 gacggcattc gacccgacgg ccgcggcccg accgacatcc gcccgctggc ggccgaagtc
      901 gacttgctgc cgcgcgtcca cggctccggc ctgtttacgc gcggcgagac gcaggtgttg
      961 tcggcggcca cgctgggcac ggcgcgtgac gcgcaggagc tggatgggct ggcgccggtc
     1021 gacgagaagc gttacatgca tcactacaac ttcccgccgt tttcgaccgg cgaagcgcga
     1081 cccttgcgcg gccagtcgcg ccgcgaggtc ggccacggcg cgctggccga gcgtgcgctc
     1141 gcggcgatgc tcccttcgat ggaagagttc ccctatacga ttcgcgtcgt cagcgaagtc
     1201 ctgtcgtcca acggatcgac ctcgatggcg tccgtgtgcg gcagcacgct ctcgctgatg
     1261 gacgccggcg tgccgatcaa agcgcccgtc gccggcattg ccatgggatt ggtcagcgac
     1321 ggcgagcgtt acaagattct gaccgacatt caggggatgg aagatcatct gggtgacatg
     1381 gacttcaaag tcgccggcac ggcgaaaggc atcactgcgc tgcagatgga cagcaagatc
     1441 aagggcctca cggctcacct cctcaaggag gcgctcgaac aggctcgcgc cgctcgcctg
     1501 caagtcctcg acgtcatgcg cagcgcgatc gccgagccgc gacccgaact gaagcctcac
     1561 acgccgcgca tcttgacgat caagatcaac cccgacaaga tcggggcggt catcggcccc
     1621 ggcggcaaga tggtgcgctc gatccaggat gagacgggcg ccacgatcga catccaggag
     1681 gacggcacga tctacatcgc cgcaaccggt gggacgagcg ccgatgcggc gcgcgcgaga
     1741 atcgagctgc tcaccgagtc gccggagctg ggccgcatct actccggcaa ggtggtgcgc
     1801 accactgact tcggcgcgtt cgtcgagatc atgcccggcg tggacggcat ggtgcacatc
     1861 tcgcagctcg ccgactatcg cgcgcccaac gtcgaatcgg tcgtcaaggt cggcgacgaa
     1921 gtgatggtca tggtcaccga catcgacgtg aacggcaaga ttcggctcag ccgcaaggcg
     1981 gtgctggagg ggatgacggt cgacgaagcc cgcgaatccg acgtgcggcg aggcggcgca
     2041 ggccgcagtg gccgaggcgg cgccggccgt agcccgcgcc gcgaaggcgg ggacgagcga
     2101 cggcggcggc gataaggaat agaggtgacc ggcgccccaa aggtgccggt caccttcaga
     2161 aacccggctt cttccaagaa accgggtttc tttcttgtga accatctgcc tttgtggtaa
     2221 aaatgactgc atgacgcctg cttcgccgag ttggtcgacc gacaccaaac gcctggtggc
     2281 gctgttcgcc gtcattttgg tcggcctgac gatctatcgt ttcaacttca tcgtcggacc
     2341 tctggctata tcgctcttga tcacctatct gtttgccccg gtcgtcggat ggatcgagcg
     2401 ccggctgcac gggcggcggg cgatcgccac cctgatcgtc ctgctgacgc tggtcatggt
     2461 actcgtcttg ctcgcggcgc tgctgattca gacggtgatc gtgcaggtgc gctcgttcaa
     2521 tctggacctc gacgcgatct acgagcggat ctccgagcgc ctcgccgagc cgctcgtcgt
     2581 cggggacgtc cgcttggact tgcgggccgc tttcgagccg ctgcgcggat cgctcagctc
     2641 gctgcttcaa acagtcgtca accgcatcgt cgacgttggc gcgaacctgg ccgaaggatt
     2701 catctggctg gtcttcatct tcatcagtac cttttacctt ctcaaggatt ggcgcgcgat
     2761 tggccgctgg tttgagaaca cgacgcctcc tggcttccgg ggcgacttta tctacctgcg
     2821 cgggcagatt gggcagacgt ggaacaactt ttttcggggg cagttggtgc tggcgctggt
     2881 gatggggacg ctggtcgggc tggcggtgtg ggtcattggc ctgcccaacg ccttcgtcat
     2941 cggcgtgttg ttcggcgtcc tggaagtcgt gcccaacttc ggcccggtga tcgcatcgat
     3001 accgaccgtg ctgatcgcgc tgttccaggg atcgagctgg atgttcgtcg gtaataacct
     3061 ggcgttcacg gtcgtcgtga tcattttgct attcttgctg caacagacgg agaacgccat
     3121 cctggtgccg cgcatcctgg ggcatcacct caacctgcat ccggtcgcgg tgctggtcgc
     3181 cgcgatcgcc ggcgcgagtc tggcgggcgt gctgggtatc ctgctggcct cgcctgtctt
     3241 ggcgacgctg cgcgacatcg cccgctacgt cggcgcgcga atgctcgacc tcgatctgta
     3301 ttcgtcgggg agtagcccgc ccggtgtata accacggatt acacagatgt cacggatggc
     3361 gacccttcaa tatctgtctg gactttggca agtgtcatgc cttgggctgg agtcgaggct
     3421 ttagccggtt ttgaccgcca gatatgacca cctaaaggct cgactccagt gatttgccag
     3481 actccagtat ctgtgtaatc cgtgtcatcc gcctgccctc gcctgccgtg tgcgggggtg
     3541 gttgaaattc ttcgcggccg gcgcgatttc tgaggagcta tactttgtgt ctggcgagtt
     3601 tgtattccgt ccggttcgtt ccgaagacaa gagccgcgtt ctagagttta cggcgcatac
     3661 ctggggcgac ggcgactaca ttcacgacgt cttcgatctc tggctggccg atacggatgg
     3721 acagttcacg gcggtcgagc tggatggacg agctgtcgcc attgccaagc tgaccgacct
     3781 cggtgagggc gaagtgtggc tcgagggcct gcgggtcgat ccggcccatc gtcgaaaggg
     3841 cattggccag gcgctgcaca attatgagct cggcctggcc cggcagatcg gcgcgcgcat
     3901 cctgcgctac gccaccggca cgaaaaacac ggtgagcctg atgttcggcg agcgcaccgg
     3961 tttccggcgc gtgtgcgact ttcgctggca cgtggccgac gcatcgacag agttctcgcc
     4021 acccgagccg ttgcgcccgg acgacctgcc gcaactgcgc gcgtggctgg attcgccctt
     4081 gctgcgatcg gcgcatgggc tgtacccgca actctggaag tggagccggc tgtcggaggc
     4141 gcgtctggtg cgtcacttgc gggccggcga ggtattcgga ttgcccggcg cttcagggct
     4201 gcgcgcctgg tcgatctgct cgctcttcga gggctgggac gaggcgcaga tgcatcacct
     4261 ggacggcgct gacccggcgt ccattgtcga gctggcgcaa tcgatgcgcg gttacgccgc
     4321 cggcggcggc cgggaaaaga ttgagtgctt cgcgctcgac ccgtcgccgc tgatcgattt
     4381 gctgcgcgac gcaggctatg cgggtgaaga cttcgccgtg acggttctcg agctggagtt
     4441 ggagcgagca tgacgaccgt gcgcgaggcg ttgcacaggc tgttccgccg gcctgaaccc
     4501 gtcgggacgg ctggactcga tcgggccggc tactcctatc gtgtgttctg gatgaagcag
     4561 gcgcgcggtt gggatgcagc gcggcgacaa tcggttttgg aggcgacgcg cgtcgtgaca
     4621 agagccgacg cgttcgagtc gaacactttc gagaggcgct atcaggtgca aggcctggcc
     4681 ggtgagcatt ccggcgcgag cctgctggcg ctcattcagg ttttggaggc gctgaatgcc
     4741 ggcgaatgaa tcggattcca acgcgcgcga ggcgctgaag cagcgcatga tccaggtctt
     4801 gcgcgagccg ctgtatgtcg ccatcgagcg cgaattcagc cgcatgctcc tgcgcgagaa
     4861 catcgtcctc tcgcgcgccg agtacagaaa gctgaagtgg gaggtcgtgc gcgccatcgt
     4921 gaacagcggc gaatgaacag aagcggggat gcccaccatg aaatgcccga actgccagca
     4981 tgacaatccg cccgaagcca cgttctgcat gaactgcggg acgaagctga gccgcacgtg
     5041 cgcgacctgc ggcgcgacgc tgcctgctgg cgcgcgtttc tgtatgagct gcggccagcc
     5101 ggccggcggc agcacgccgg ccgacgaagc gcgcctggcc cgcctcgccg ccgctgcgcc
     5161 ggtgccgctg gctgagaaga tgcgtgctgc ccacctggaa ggtgagcgca aacaagtgac
     5221 cgtcttgttc gccgacgtag tcggctcgac cgccttggca gagcgaatgg accctgaaga
     5281 atggacgacg atcatgaacc gcgccttcga gcagttgtcg cccgcgattt accgctacga
     5341 aggcacgatc gcgcgtctga tgggcgatgc gcttctggct ttcttcggcg cgccgctcgc
     5401 ccacgaggac gatcccatgc gcgcgctccg cgccgcgctc gatctgttga aagcggcgcg
     5461 ggcatacggc gacgagctgc gccgcgagcg cggcatt
//
LOCUS       DBNHXB010000231         6287 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1228065, whole genome shotgun sequence.
ACCESSION   DBNHXB010000231 DBNHXB010000000
VERSION     DBNHXB010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6287)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1228065"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="AC162G_09510"
     CDS             <1..218
                     /locus_tag="AC162G_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076093.1"
                     /translation="KVDSSGTTAYVGATEALITGTQRITRTYYSAGAQLVAMRVVTST
                     GGNALREAYLHADHPSASSGQVWAARL"
     gene            complement(296..964)
                     /gene="rnc"
                     /locus_tag="AC162G_09515"
     CDS             complement(296..964)
                     /gene="rnc"
                     /locus_tag="AC162G_09515"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019498574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR5076094.1"
                     /translation="MSSLPTFRDPSLLERALTHRSYLNERPELAIEDNERLEFLGDAV
                     LDFVTGAWLYNRFPELQEGRLTSLRAALVKTDALADFARRVGVDEHLRLGKGESETGG
                     RGRATLLADAFEAVLGALYLDQGLEAVQAYLEPLLPDEVERILLEERDRDAKSVLQEW
                     SQAERAITPRYRTVAEEGPDHAKLFTVEVRIGDEIVGRGTGLSKQAAAQAAASDALRR
                     LSVW"
     gene            complement(1006..2250)
                     /gene="fabF"
                     /locus_tag="AC162G_09520"
     CDS             complement(1006..2250)
                     /gene="fabF"
                     /locus_tag="AC162G_09520"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR5076095.1"
                     /translation="MNQRRVVITGMGTVNPLGLDPETTWKNALAGASGVGPITRFDSS
                     PLQVHIAAEVKNFDPTQYMDAKEARRRDPVTQFAVVAAMQAVQMSGLKVDNSFGDEVG
                     VYIGTGVGGLQTMHDGTQLVLLEGPRRISPFIIPMFLADSPASTVAIQYGARGLNFSP
                     ATACAAGSDAIGMALRDIQRGEARAVIAGGSEAPIVMVGMAAFDRTGACSRMNDEPQR
                     ASRPFDKNRQGLVFGEGAAVFVVEALEHALARGARPLAEIAGYGASSDAYHMTAPLED
                     GSGAAIAIRKALHDASLAPTDIDWINAHGTATPLNDRMETLAIKSVFGEHAYNVPVTS
                     TKSMHGHIMGATGALGVMWCMLAIRDGLIPPTINYETPDPECDLDYTPNQARRRTVDA
                     AMTNAFGFGGHNSVLIIKRYVE"
     gene            complement(2273..2992)
                     /locus_tag="AC162G_09525"
     CDS             complement(2273..2992)
                     /locus_tag="AC162G_09525"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014448.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030366 - molybdopterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein"
                     /protein_id="HIR5076096.1"
                     /translation="MRIDVQLFATLRDRAGSKAISVEIGDVATVEQLLDHIAAAYPAL
                     APALPSAIVAVNQEFAFLPTLLHTGDEVALFPPVSGGADYPEVFRVTPDAIDLNVLVA
                     QVTLPTTGAVCTFTGTVRGRSPERETVQLDYEAYTPMAEAKMRQVAREIRARWPKVEG
                     IAMVQRTGPLKVGQFTVLIAVSAGHRYDGVFEAARYGIDRLKEIVPVWKKEIGPGGEE
                     WIEGHYHPAPVDAEEAPAYEP"
     gene            complement(3029..3526)
                     /gene="moaC"
                     /locus_tag="AC162G_09530"
     CDS             complement(3029..3526)
                     /gene="moaC"
                     /locus_tag="AC162G_09530"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR5076097.1"
                     /translation="MTTQDERRLTHVDEQGHARMVDVGAKPDTRRAAVAKGEVHLSPE
                     TLRQIVAGVTPKGDVFATARIAGILAAKKTSELIPLCHPLALTHVAVDLQAEGDRVIE
                     ITARVETTGKTGVEMEALTAVSVAALTVYDMIKALQRDARITNVRLVEKHGGKSGDVV
                     LEAGG"
     gene            complement(3523..4260)
                     /locus_tag="AC162G_09535"
     CDS             complement(3523..4260)
                     /locus_tag="AC162G_09535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5076098.1"
                     /translation="MVSLSIVIPAYNEEANVAEAVRRVSRAAQNLGMDYEIIVVDDGS
                     RDRTGDAVREMTPSTPNLRLVEHFPNRGYGGALKAGFAASTKDLIAFLPADNQFDFSE
                     IRLLLDKLAPDVVLVSGHRVNRRDNVVRKFNAFGWNLVVRLMFGYLIRDIDCGFKVFR
                     RWVLDRIHVESNGAMIDTEMLAEIRARGYRIAEAPVSHLPRTAGSPTGANLKVVLRAF
                     RDLFRFRLRLWRELREERRAARTDVKP"
     gene            4333..5247
                     /gene="ftsY"
                     /locus_tag="AC162G_09540"
     CDS             4333..5247
                     /gene="ftsY"
                     /locus_tag="AC162G_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR5076099.1"
                     /translation="MRDRLTAFQETLARTRRAAFGRVATLLGATEVTPNLWDELEAAL
                     IQADVGVATTSDLLQRLRARAAREGMLRAADLQSALKDELRRMLAAPANGSRQLDTAR
                     PAVALIVGTNGSGKTTSIAKLASRHKRDGRKVLLVAADTFRAAAGEQLDVWADRAGVE
                     LIGGRPGADPGAIAFDALQAAQARGFDVVLVDTAGRLHTKYNLMQELKKVRNVIGKTL
                     PGAPHATYLVLDGTTGQNALTQARQFKAAVDVTGVIVTKLDGTARGGMVFSITHELGL
                     PVAYIGTGEKIDDIEPFDAQAFVDSLFE"
     gene            5274..>6287
                     /gene="prfB"
                     /locus_tag="AC162G_09545"
     CDS             join(5274..5339,5341..>6287)
                     /gene="prfB"
                     /locus_tag="AC162G_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143597621.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR5076100.1"
                     /translation="MEELRASLIELNSQIASVMERLDVAGKQQRIRELEKQASASDFW
                     SDAQSAQAVMRELNALKSEIQEWVDQSRRARDALELLELAETDAALADGIAEELDAIR
                     RAFEVSEFHLLLSGSHDRGDAIVAIHAGAGGVDAADWTAMLLRMYLRWAESRGFKAEV
                     LDQLEGDEAGLRSVTFSVAGEYAYGYLRAEKGVHRLVRLSPFDAAHRRHTSFALVEVW
                     PDLPDNSEIVVNPSDVKIETFRASSAGGQHVQKNATAVRITHLPTGIVVSCQNERSQT
                     QNRETALKILRAKLYEIQEENQRKALAKLKGEHVKAEWGSQIRSYVLHPYQMVKDHRT
                     GHEV"
BASE COUNT         1075 a         2083 c         2045 g         1084 t
ORIGIN      
        1 agaaggtgga ctcaagcggc acgacggcct atgtcggcgc gaccgaggcg ctcatcaccg
       61 gcacgcagcg catcacgcgg acgtactaca gcgccggggc gcagttggtg gcgatgcggg
      121 tggtgacctc gacgggcggg aatgcgctac gcgaagcgta tctgcatgcc gaccatcctt
      181 cggcaagctc aggacaggtc tgggcggcac gtctttaata accaaatgcc gacggacgtg
      241 ggctacaccg gccaacgcca cgacgctcag atagcagcac gactgcccgc gcatactacc
      301 acacgctcaa ccgccttagc gcatcactcg ccgctgcctg cgccgccgcc tgcttgctca
      361 ggcccgtccc cctgccgacg atctcgtcgc cgatgcgcac ttcgacggtg aacagcttgg
      421 cgtgatccgg cccttcttcg gcgacggtgc ggtagcgcgg ggtgatggcg cgctcggcct
      481 ggctccactc ctgcagcacg ctcttcgcgt cgcggtcgcg ctcctcgagc agaatgcgct
      541 cgacctcgtc cggcagcagc ggctctagat acgcctgcac cgcctccagg ccctggtcga
      601 gatacagcgc gccgagcacc gcctcgaacg cgtcggccag cagcgtggcg cgcccgcgcc
      661 cacccgtctc cgattcgcct ttgcccagcc gcagatgctc gtctaccccg acgcgccgcg
      721 cgaaatcggc gagcgcgtcg gtcttcacca gcgccgcgcg cagcgaagtc aaccggccct
      781 cctgaagctc ggggaagcgg ttgtacagcc acgcgcccgt cacgaagtcg agtaccgcgt
      841 cgcccagaaa ctccagccgc tcgttgtctt cgatcgccaa ttcgggacgc tcgttcaagt
      901 aagagcgatg agtcagcgcg cgctcgagca gcgagggatc gcgaaaggtg ggcagtgaag
      961 acatgctgga agcgtgagcg tgatacgtga tcacgtgatc acgtgtcact ctacgtagcg
     1021 cttgatgatg agcacgctgt tgtgcccgcc gaagccgaag gcgttcgtca tagcggcgtc
     1081 gacggtgcgc cggcgcgcct ggttaggcgt gtagtccagg tcgcattccg gatcgggcgt
     1141 ctcgtaattg atcgtcggcg ggatcaggcc gtcgcggatg gcgagcatgc accacatcac
     1201 gccgagagcg cccgtcgcgc ccatgatgtg gccgtgcatc gacttggtgc tggtgacggg
     1261 gacgttgtag gcgtgctcgc cgaaaacgga cttgatggcc agcgtctcca tgcgatcgtt
     1321 gagcggcgtc gccgtcccgt gcgcattgat ccagtcgata tcggtcggcg ccaggctcgc
     1381 atcgtgcagc gccttccgga tggcgatcgc cgcgcccgac ccgtcttcga gcggcgccgt
     1441 catgtgatac gcgtcggacg atgcgccgta gcctgcgatc tcggccaacg gcctcgcgcc
     1501 gcgcgccagc gcgtgctcga gcgcttccac cacgaagacg gccgcgccct cgccgaacac
     1561 cagaccctgc cggttcttgt cgaacggccg gctggcgcgc tgcggctcgt cgttcatgcg
     1621 cgagcaggcg cccgtacggt cgaaggccgc catgccgacc atcacgatcg gcgcttccga
     1681 cccgcccgcg atcaccgcgc gtgcctcgcc gcgctggatg tcccgcagcg ccatgccgat
     1741 ggcgtccgag ccggcggcgc atgcggtcgc cggcgagaaa ttcaacccgc gcgccccgta
     1801 ttgaatcgct accgtgctag caggactgtc ggcgaggaac atcgggatga tgaatgggct
     1861 gatccgtcgc ggcccttcca gcaggacgag ctgcgtccca tcgtgcatcg tctgcagccc
     1921 gccgacgccg gtgccgatgt acacgcccac ctcgtcgccg aagctgttgt ccactttcag
     1981 acctgacatt tgcacggctt gcatcgccgc cacgacggca aactgtgtca cggggtcacg
     2041 acgccgcgct tccttggcgt ccatgtactg cgtgggatcg aagttcttca cttcggcggc
     2101 gatgtgaacc tgcaacgggc tcgagtcgaa ccgcgtgatc ggtccaaccc ctgatgcgcc
     2161 cgccagggcg ttcttccagg tggtctctgg gtccagcccc aacgggttga ccgtgcccat
     2221 cccggtgatc acgacacgtc gctgattcat agtcccccca gaacgagttt tgtcagggct
     2281 cgtacgccgg tgcctcttcc gcgtcgacgg gcgccggatg gtagtggccc tcgatccact
     2341 cctcgccgcc cggcccgatc tcctttttcc agactggcac aatctccttg agccggtcga
     2401 tgccgtatcg agcggcttcg aacaccccat cgtagcggtg gcctgccgac accgcaatca
     2461 gcaccgtgaa ctggcccacc ttcagcggcc cggtgcgctg caccatggcg atgccttcca
     2521 ccttcggcca gcgcgcgcgg atttcgcgcg ccacctggcg catcttggcc tccgccatgg
     2581 gcgtgtaggc ttcgtagtca agctgaacgg tttcgcgctc cggggacctg ccgcgcacgg
     2641 tgccggtgaa cgtgcacacc gcgccggtcg tcgggagtgt cacttgcgcg acgagcacgt
     2701 tcaagtcgat ggcgtctggg gtgacgcgga atacttcggg gtaatccgct ccgccgctga
     2761 ccggtggaaa cagcgcgacc tcgtcgcccg tgtggaggag ggtaggtagg aacgcgaact
     2821 cctggttcac ggcgacgatg gcgctgggca gcgccggcgc cagcgccggg tacgcggcgg
     2881 cgatgtggtc gagaagctgc tcgacggtcg ccacgtcgcc tatctcgacc gaaatagcct
     2941 tgctgccggc gcgatccctg agcgtagcaa acagttgaac gtcgatgcgc attccaacct
     3001 ccaacctcca ataaccaatc accactccct agcctccagc ctccaacacc acatccccac
     3061 tcttgccgcc gtgtttctcg accagccgca cgtttgtgat gcgcgcgtcg cgctgcagcg
     3121 ccttgatcat atcgtacacc gtcagcgccg ccacgctgac ggcggtcagg gcttccattt
     3181 cgacgccggt cttgcccgtc gtctccacgc gcgccgtgat ctcgatcacc cggtcaccct
     3241 ccgcctgcaa atcgacggcg acatgcgtca aggccagcgg atggcacagc gggatcaact
     3301 cgctcgtctt cttcgcggcc aggatgcccg cgatgcgcgc cgttgcgaac acgtcgccct
     3361 tcggcgtcac gccggcgacg atctggcgca gcgtctcggg cgacaggtgc acttctccct
     3421 tcgcgaccgc cgcgcgccgg gtatccggct tggcgccgac gtcgaccatg cgcgcgtgac
     3481 cctgctcgtc gacatgcgta aggcgacgct cgtcctgggt cgtcatggct tcacatccgt
     3541 ccgggcggcg cgccgctcct cacgcagctc gcgccacagc cgcaggcgaa agcggaacag
     3601 gtcgcggaag gcgcgcagga cgactttgag attcgcgccc gtcggcgagc cggccgtgcg
     3661 cggcagatgg ctcaccggcg cttccgcgat gcgatagcct cgcgcgcgta tctcggccag
     3721 catctccgtg tcgatcatcg cgccgttcga ttcgacgtga atgcggtcga gcacccagcg
     3781 gcgaaacacc ttgaacccgc agtcgatatc tcggatcagg taaccgaaca tcaggcgcac
     3841 caccaggttc catccaaacg cgttgaactt ccgaacgaca ttgtccctgc gattgacgcg
     3901 gtggccgctg accagaacca cgtccggcgc cagcttgtcg agcagcaggc ggatctcgga
     3961 aaagtcgaac tggttgtcgg cgggcaggaa ggcgatcagg tctttcgtcg acgccgcgaa
     4021 gcccgccttg agcgcgccgc cgtagccccg attggggaaa tgctcgacca gccgcaggtt
     4081 gggcgtggat ggcgtcatct cacggacggc gtcccccgtg cgatcgcggc tcccgtcgtc
     4141 gacgacgatg atttcgtagt ccatccctag attctgtgcg gcgcgggaca cgcgccgaac
     4201 ggcctcggcg acgttggcct cttcgttgta ggccggtatg acgatggaaa gactaaccat
     4261 tcgcgcctcg gacctcgcag ccgattatat cacaggcgtg tatcgtgcga tggccgtggt
     4321 ataatctttc tgatgcgcga tcgattgact gctttccaag aaacactggc gcgtacccgc
     4381 cgcgcggcgt tcggccgtgt cgccacgctg ctcggcgcga cggaggtcac acccaacttg
     4441 tgggatgagc tagaagccgc cttgattcag gccgacgtgg gagttgccac gacaagcgac
     4501 ctgcttcagc ggctccgcgc acgcgccgcg cgggagggta tgctgcgcgc cgcggatttg
     4561 cagagcgcgc tcaaggatga gctgaggcgc atgcttgccg cgccggcgaa cgggtctcgc
     4621 cagctcgaca ccgcgcggcc ggcagtggcg ctgatcgtgg ggacgaacgg ctccggcaag
     4681 acgacgagca tcgccaagct ggcttctcgc cataagcgag acgggcgcaa agtgctgttg
     4741 gtggcagccg acacgttccg cgcggcggcc ggagagcagc tcgacgtgtg ggctgaccgg
     4801 gcgggcgtcg aactgatcgg cgggcggcct ggcgccgatc cgggcgcgat tgcgttcgac
     4861 gcgctgcagg cggcccaggc gcgcggattc gacgtcgtgc tcgtcgatac ggcaggccgg
     4921 ctgcatacca aatataacct gatgcaggag ctcaagaagg tacgcaacgt gatcggtaag
     4981 acgctgcccg gcgcgccgca cgcgacctat ctggtgctgg acggcacgac tggccagaac
     5041 gcgctgacgc aggcccgcca gttcaaagcc gccgtggacg tgacgggcgt gatcgtcacg
     5101 aagctcgacg gcacggccag gggcggcatg gtgttctcga tcacgcacga gctggggctg
     5161 ccggtggcct acatcggcac gggtgagaag atcgacgaca tcgagccatt cgacgcgcaa
     5221 gcattcgtcg atagcctgtt cgagtgagtc gtttttgcaa aggagtacga tatatggaag
     5281 agctgagagc gtcgctgatc gagctgaatt cgcagattgc atccgtcatg gagcgtcttt
     5341 gacgtcgcag gcaagcagca gcgcattcgc gagctggaga agcaggcgag cgcctccgat
     5401 ttctggagcg acgcccagtc ggcgcaggct gtgatgcgcg agctgaatgc gctgaagtcg
     5461 gagattcaag aatgggtcga tcagtcgcgc cgggcgcgtg acgcgcttga attgctggag
     5521 ctggcggaga cggatgcggc gctcgccgac gggatcgccg aagaactcga tgcgatccgg
     5581 cgcgcgtttg aagtcagcga gttccatctg ctgctatcgg ggtcgcacga tcggggcgac
     5641 gcgatcgtcg ccatacacgc gggagcgggc ggcgtcgacg ccgccgactg gactgcgatg
     5701 ctgctgcgca tgtatctgcg ctgggcggag agccgcgggt tcaaggcgga agttctggat
     5761 caactcgaag gcgacgaggc cgggctgcgc agtgtgactt tctccgtcgc cggcgaatac
     5821 gcgtacggtt acctccgcgc tgagaaaggt gttcatcgcc tggtgcgcct ctcgccgttc
     5881 gacgctgcgc accggcggca tacgtccttc gcgctggtcg aggtctggcc cgacctgccc
     5941 gacaacagcg agatcgtcgt caacccgagc gacgtcaaga tcgagacgtt tcgggcatcc
     6001 agcgcaggcg ggcagcacgt gcagaagaat gcgacggcgg tgcgcatcac tcacctcccg
     6061 acggggatcg tcgtgtcgtg ccagaacgag cgcagccaga cgcagaatcg cgagacggcg
     6121 ctcaagattc tccgcgccaa gttgtacgag atccaggagg agaatcagcg caaggcgctg
     6181 gccaagctga agggcgagca cgtcaaggca gagtggggca gccagattcg ctcatatgtt
     6241 ctacatccct atcagatggt caaggatcat cgcaccggcc acgaagt
//
LOCUS       DBNHXB010000232         4409 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3912023, whole genome shotgun sequence.
ACCESSION   DBNHXB010000232 DBNHXB010000000
VERSION     DBNHXB010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3912023"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="AC162G_09550"
     CDS             complement(<1..733)
                     /locus_tag="AC162G_09550"
                     /inference="COORDINATES: protein motif:HMM:NF048867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kelch repeat-containing protein"
                     /protein_id="HIR5076101.1"
                     /translation="MKRQLLTLALAVAFVAACGPAPAPTQAPAPTSPPPPAAPIEPTR
                     PVMVPTSAGISPPGSGGPAIVPTKAAAATPLAGQVPAGRTAPGWKRLDTANPPPARFD
                     YTLALDAASRKLVLFGGRDGSQTLGDTWVFDLASSTWREVKASPSPGARFGHSATYDP
                     KSKRVLIFAGQAGGFFNDVWAFDAAKETWQKLETKGTAPAQRYGSSAIVDTKTNQLII
                     THGFSSSRFDDTFALDLTTNTWSAIV"
     gene            929..1846
                     /locus_tag="AC162G_09555"
     CDS             929..1846
                     /locus_tag="AC162G_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR5076102.1"
                     /translation="MNTPAGRSPLFNYGGQAVIEGVMMRGSKAMAVAVRNPKQEIVIH
                     TQPLSQKLYGGRMMRIPFLRGLTMLWDALGLGMRSLMYSADVALGEEEVKFSGPVAWG
                     TIAVSLAFGLGLFVFLPKGVAALFEPSMTPLASALVEGAVRLALFVAYIWLIGWMKDI
                     RRVFGYHGAEHKTINAYEAGAELTVRSVQRFPLAHPRCGTAFLLTVVVLSIFFFAPLN
                     ALGLPFGVSLALRLALIPVVAMVAYEYIRFSARHLDNALVRAIIIPNLALQSLTTRQP
                     DDSMVEVAIAAFNKVLESEGTGAVSAAVR"
     gene            1913..2842
                     /gene="mdh"
                     /locus_tag="AC162G_09560"
     CDS             1913..2842
                     /gene="mdh"
                     /locus_tag="AC162G_09560"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR5076103.1"
                     /translation="MRNKITVVGAGNVGASCALWLAERELGDIVLVDIPQTGTMPMGK
                     SLDLLQSGPVYGFNTRITGAIDYGPTANSDVVVITAGFPRKPGMSREDLVGKNQEIIT
                     DVMGQVTKTSPNAIVIVVTNPLDTMVYLAHKLSGWSRSRVFGQAGQLDSARMAAFIAL
                     ELDVSVSNVHASVMGGHGDEMVPLVRYTTVAGVPIGELLPPERVQAIVERTRKGGGEI
                     LNLLGTTAYYAPGAAAARMVEAILKDQKLILPCSVVLEGEYGLRDIAFGVPVKLGRGG
                     VERIFEVRLDDDERVMLQKSVDLIRETMSALKM"
     gene            2929..>4409
                     /gene="pckA"
                     /locus_tag="AC162G_09565"
     CDS             2929..>4409
                     /gene="pckA"
                     /locus_tag="AC162G_09565"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR5076104.1"
                     /translation="MIQSKNGLELAGLHPSGTLHWNLSPALLVEHAVRRAEGAITRDG
                     PFNAITAPYTGRSPGDKFTVREPSSENDVWWGKQNQALSPEHFERLYQKVVAYLSGCG
                     VLYVRDLYAGADPAYRLKVRVINENAWQNLFVYNLFIRPDAAGLADFMPDFTILHAPG
                     CKADPVTDGTRTETFIVLNFARKMILIGGTHYAGEMKKSIFTTLNYLLPMHNVFPMHC
                     SANVGKHGDTALFFGLSGTGKTTLSADPQRGLIGDDEHGWSDRGVFNFEGGCYAKVIR
                     LSAEAEPEIYATMRRFGTVLENVVVDPESRALDLDDESITENTRAAYPIDFIGNAVIP
                     GLGDHPKNVMFLAADAFGALPPISKLTPELAMYHFLSGYTAKLAGTERGVTEPQATFS
                     TCFGAPFLPRHPTVYARMLGEKIAKHKAQVWLVNTGWSGGPYGVGARMKISYTRAMVH
                     AALSGSLDRVPTEADPIFGLHMPVECPGVPSEVLNPRRTWPDP"
BASE COUNT          752 a         1437 c         1409 g          811 t
ORIGIN      
        1 gcacgatcgc cgaccaggta ttcgtcgtca ggtcgagcgc gaacgtatcg tcgaagcggc
       61 tgctggagaa tccgtgcgta atgatcagtt ggttcgtctt cgtgtcgacg atggccgagc
      121 tgccgtaccg ctgcgccggg gctgtcccct tcgtctccag cttctgccag gtctctttcg
      181 ccgcgtcgaa ggcccacacg tcgttgaaga agccgcctgc ctgtccggcg aagatcagca
      241 cgcgctttga ctttgggtcg taagtggcgg agtgcccgaa gcgcgcccct ggcgagggcg
      301 aggctttcac ttcgcgccac gtactcgagg cgagatcgaa gacccaggtg tcgcccagcg
      361 tctgcgagcc gtctcgcccg ccgaaaagga cgagctttct gctggccgcg tcgagcgcga
      421 gcgtgtagtc gaagcgggca gggggcgggt tcgccgtatc aaggcgcttc cagccgggcg
      481 cagtgcggcc ggcgggaact tgcccggcaa gcggtgtcgc cgctgccgcc ttcgtcggca
      541 cgatggccgg cccgcccgaa ccgggtgggc tgattccggc gctggtagga accatcacgg
      601 gccgagttgg ctcgatcggg gcagcaggcg gtggaggcga tgtcggcgct ggagcctgcg
      661 tcggcgctgg ggccggcccg caggcggcga cgaaagccac agccagcgct aaagtaagca
      721 gttggcgttt catgtctcct ccttacgtca tcattttaca gacgcagttt gccagtcaat
      781 tgttgcgaca aagttacttc agcgctgcca aatcatagaa cagcccacat cgcgcatcgc
      841 aatccaatgt ccgtgcaatc cgcgttcatc cgtgtccgag cactcgctat gcttgacaca
      901 atctggcgcg ctgggtagaa tcgcggtcgt gaatactccg gcggggcgct cgcccctatt
      961 caactacggc ggccaggctg tcatcgaagg cgtgatgatg cgcggctcga aggcgatggc
     1021 cgtcgccgtg cgcaacccca agcaggaaat cgtcattcat acgcagccgc tcagccagaa
     1081 gctatacggc gggcgcatga tgcgcatccc gttcctgcgc ggcttgacga tgctgtggga
     1141 cgcgctcggc ctcgggatgc gcagtctcat gtattcggcc gacgtcgcgc tgggcgaaga
     1201 agaggtgaag ttcagcgggc cggtggcgtg gggcacgatc gccgtgtcgc tcgccttcgg
     1261 tctcggcctg ttcgttttcc tccccaaggg cgtcgccgcc ttattcgagc cgtcgatgac
     1321 gcccctcgcc tctgcgctcg tcgaaggcgc cgtgcggctc gccctgttcg tcgcatacat
     1381 ctggctcatc ggttggatga aggacatccg gcgcgtgttc ggctatcacg gcgcggagca
     1441 caagacgatc aacgcctacg aggccggcgc ggaactgacc gtgcggagtg tccagcgctt
     1501 cccgctcgcg catccgcgct gcgggacggc cttcttgctg accgtcgtcg tgctctcgat
     1561 cttcttcttc gcgcccctca acgcgctcgg cctgccgttt ggcgtatcgc tggctctgcg
     1621 cctggcattg atcccggtcg tggcgatggt cgcctacgag tatatccgat tctcggcgcg
     1681 gcatctggac aacgccctcg ttcgcgcgat catcatcccc aacctggctt tgcagtcgtt
     1741 gaccacgcgc cagcccgatg actcgatggt cgaggttgcg atcgccgcgt ttaacaaggt
     1801 gctggagtcg gaagggacag gcgcggtatc cgctgcggtc cgctgagcgt gtttcgtgtt
     1861 gcgtgttccg tcacgcaaca cgcactacgc aacacgtttt caaggaggaa tcatgcgcaa
     1921 taaaatcacc gtcgtcggcg cgggcaacgt cggcgcgtcg tgcgcgctgt ggctggccga
     1981 acgcgagctg ggcgatatcg tgctggtgga catcccgcag accggcacga tgcccatggg
     2041 caagtcgctc gacctgctcc aatccgggcc ggtctacggc ttcaacacgc gcatcaccgg
     2101 cgccatcgac tatggcccca ccgccaactc ggacgtggtc gtgatcacgg ccggcttccc
     2161 gcgcaagccc ggcatgagcc gcgaggacct ggtcggcaag aaccaggaga tcatcaccga
     2221 tgtcatgggg caggtgacga agacttcccc gaatgccatc gtcatcgtcg tgaccaaccc
     2281 actcgacacg atggtgtacc tggcgcacaa gctgagcggc tggtcccgct cgcgcgtctt
     2341 cggccaggcg ggccagctcg attcggcgcg catggcggct ttcatcgccc tggagctgga
     2401 cgtctcggta tccaacgtgc atgcctcggt gatgggtggg cacggcgatg agatggtgcc
     2461 gctcgtgcgc tacacgaccg tcgcgggcgt gcccattggc gagctgctac cgccggagcg
     2521 cgtgcaggcc atcgtcgagc gcacgcgcaa gggcggcggc gagatcctca acctgctggg
     2581 cacgaccgcg tattacgcgc ccggcgcggc cgctgcccgc atggtcgagg ccatcctcaa
     2641 agatcaaaag ctcatcctgc cgtgctcggt cgtcctcgag ggcgagtatg gcctgaggga
     2701 catcgccttt ggcgtgccgg tgaagctcgg gcgcggcggg gtcgagcgca tcttcgaggt
     2761 ccggctcgac gacgatgaaa gggtgatgct gcaaaagtcg gtcgatctca ttcgggagac
     2821 gatgtccgcg ctcaagatgt agacgagttc ctttagtgcc aggcctgccc tggaatggga
     2881 ctcaaggaac ttaaggaact caaggaactc aaggcctcaa ggacgattat gattcaaagc
     2941 aaaaatgggc tggaattggc cgggctgcac ccttccggca cactgcactg gaatctctcg
     3001 cccgcgctgc tcgttgagca cgccgtccgg cgggccgagg gcgccatcac gcgcgacggc
     3061 ccgttcaacg ccatcaccgc gccgtacacc ggccgctcgc ccggcgacaa attcaccgtg
     3121 cgcgagccgt cgagcgaaaa tgatgtctgg tggggcaagc agaaccaggc gctcagcccc
     3181 gagcatttcg agcggcttta tcagaaggtc gtcgcctacc tgagcggctg tggcgtgcta
     3241 tacgtgcgcg atctgtatgc gggcgccgat cccgcgtatc gcctcaaagt gcgcgtcatc
     3301 aacgagaacg cctggcagaa tctcttcgtc tacaacctgt tcatccgccc cgacgcggcc
     3361 ggcctggccg actttatgcc cgactttact atcctgcatg ccccgggctg caaggccgat
     3421 cccgtgaccg acggcacccg caccgaaacg ttcatcgtcc tcaacttcgc gcgcaagatg
     3481 atcctgatcg gcggcacgca ctacgcgggc gagatgaaga agtcgatctt caccacactc
     3541 aactacctgc tgccgatgca caacgtgttc ccgatgcact gctcggccaa tgtcggcaag
     3601 catggcgaca cggcgctgtt cttcggcctg tcgggcacag gcaagaccac gctgtccgcc
     3661 gatccgcagc gcgggctgat cggcgacgac gagcacggct ggtcggatag gggcgtgttc
     3721 aacttcgagg gtggctgtta tgccaaggtg atccgcctct ccgccgaagc cgagccggag
     3781 atctacgcga ccatgcgccg cttcggcacg gttctcgaga atgtcgtcgt cgatcccgaa
     3841 tcgcgcgcgc tcgatctcga cgacgagtcg atcaccgaga acacgcgcgc ggcatatcct
     3901 attgacttca tcggcaacgc ggtcatcccc ggcctgggcg atcatccgaa aaacgtgatg
     3961 ttcctggccg ccgacgcctt cggcgcgctg ccgccgattt ccaagctcac gccggagctg
     4021 gcgatgtatc acttcctgtc cggctacacg gcgaagctgg ccggcaccga gcgcggcgtg
     4081 accgagccac aggcgacatt cagcacctgc tttggtgcgc cgttcctgcc acgccatccc
     4141 accgtctacg ctcgcatgct gggcgagaag atcgcgaagc acaaggcgca ggtctggctc
     4201 gtgaataccg gctggagcgg cgggccttac ggcgtcgggg cgcgcatgaa gatcagctac
     4261 acgcgtgcga tggtgcacgc ggcgctgagc gggtcgttgg accgggtgcc caccgaggcc
     4321 gacccgatct ttggcctcca catgccggtc gagtgccccg gcgtcccgtc cgaggtgctc
     4381 aatccgcgcc gcacgtggcc cgacccgaa
//
LOCUS       DBNHXB010000233         9277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2571948, whole genome shotgun sequence.
ACCESSION   DBNHXB010000233 DBNHXB010000000
VERSION     DBNHXB010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9277
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2571948"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="AC162G_09570"
     CDS             complement(<1..302)
                     /locus_tag="AC162G_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076105.1"
                     /translation="MPHKERILCALRFSFVVLRRAGIACLILVLLAPSTAIAIRQTSG
                     DAAAGARSTASSSYVYLPAIHKSGCAPIPGVSYGAVAPDPVIGIDPQNDYRINLALR"
     gene            complement(442..3078)
                     /gene="recG"
                     /locus_tag="AC162G_09575"
     CDS             complement(442..3078)
                     /gene="recG"
                     /locus_tag="AC162G_09575"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR5076106.1"
                     /translation="MTGRPASFEKLTKILKLEAEFGYRDRAMIGGLAQYAPKWQEEAR
                     RESGDAQRIEAITERLRRYGAIDDIEARRQSVEDMLSLLSLATAGVADTPGAPYSQAA
                     AHPQPPSTPSPATTTAGETREEERHGDAVPPVARRPAVPAAPAEAVAAPRPADSVTPE
                     GPSQNGMGTPFLQEHRRPARGERATLDSPATAIPGVGPSIAEKLGRLGVKTIRDLLFF
                     LPRRYDDFTALRPINRLQYGEEVTIIGSVWEVGVRHTRGGAQIVQAVISDGSGTIQCT
                     WFNPYLKDKIRRGQQIVVSGKVTEYLGRLTFQSPEWELLEKDLLHTARLVPIYPLTEG
                     LTGRGVRKLMRRAIDEWAGRIPDFLPDSVLASAGLFSLPKAIQQAHFPDSTTQLDAAR
                     KRLAFDELFMLQMGLLRQRRRWKSQNGRAIDVSPATLDQFLGALPYALTAAQRRALDD
                     VLSDMRSGQPMNRLIQGDVGSGKTIVAAAAMFAVTQAGAQAAIMAPTEILAEQHYQNF
                     TRVFANLGAIGLAQPVSIHLLTGSTPHADREALLAGVASGHAHILIGTHALIQQGVEF
                     RDLAFVVIDEQHRFGVQQRAALRQKGVKPEEGPASPHMLVMTATPIPRTLALTLYGDL
                     DLSVIDEMPPGRQPIVTRVVLPTERERMYSFIRSQIEKGRQAYIICPLVEESENIEAK
                     AAVEEHERLQKHIFPQFKLGLLHGRMKSEEKDAVMRAFAANELNILVSTSVVEVGIDV
                     PNATVMLIEGANRFGLSQLHQFRGRVGRGTEKSYCLLLADSTANGMNERLQAVEATQD
                     GFALAEKDLELRGPGEFLGTRQSGLPDMRLANIADARLIDLARREAEKLISADPNLEA
                     PEHGALAQRLHAFWSSGTGDVS"
     gene            complement(3075..3926)
                     /locus_tag="AC162G_09580"
     CDS             complement(3075..3926)
                     /locus_tag="AC162G_09580"
                     /inference="COORDINATES: protein motif:HMM:NF014679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR5076107.1"
                     /translation="MVRIVTDSTADVPEEFVRELDIVVVPVHVIFGTRSYDDGVNLSR
                     EEFYARLITADPLPTSSSPSAGEFEVTYRRLRDEGADAVVSVHVAATLSAVQNAAHMG
                     AQAVPDLEVAIVDSGQVSMGLGWQVIEAARAARAGQSIGEILDAVARVRQRVRLFAAL
                     DTLEYVRRSGRVGWARAAIGQLLKIKPIVEVRDGAVLSVDRVRTRAHCLARLKELVAG
                     QGALRSLIVLHTRARNAAQALADEFRTLHSSLSDPIYVAEATTAIGTYVGPNGLGVAC
                     VVDERRK"
     gene            complement(3966..4829)
                     /locus_tag="AC162G_09585"
     CDS             complement(3966..4829)
                     /locus_tag="AC162G_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR5076108.1"
                     /translation="MTRVHVITDSGAHFADPGVVERLGITVVPQIIQLGAQHFREGVD
                     LGAEDFYRRLPHFIGTPECHPPSVDEFRALYASLIRRNEQVLSIHTSSRMNQTIARAR
                     QAADEFLGRGKIVVIDSMHTSLGLGILIEAAAEAAARGEPLDEMVRIVRGMIPHMYAV
                     YFTDSLDYLERGKRLSKSQSILGAMLGIKPFLSVEDGEIIPMEKVRTREKAIEKLVEF
                     VSEFSAIERVAIMQSGAQPTEDTRLLLERLEQTFPGQYFPVMMYGASLACLIGPNSLG
                     IVIYEGAAPRA"
     gene            complement(4836..6488)
                     /locus_tag="AC162G_09590"
     CDS             complement(4836..6488)
                     /locus_tag="AC162G_09590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIR5076109.1"
                     /translation="MASAVDQPLYALSGLQLKEMVQASVSWLRVNQQSVNALNVFPVP
                     DGDTGTNMLLTMQAAWAEVEKVDGATIHRMAHAVAHGALMGARGNSGVILSQLWRGFA
                     RGLGSKPAMNAADFVVAMHEGCRTAYQGVIRPVEGTILSVSRAAADAAEASAKTTDDL
                     MLIFEQVLVAAQEAVARTPTQLEVLRQAGVVDAGGQGLAVIFEGMLRHLKGEPVEMEP
                     IVATLAFAPSTMLESIEEGQDYELIFDLKPRPDLDWDAMHQELSTFGTSIQVGSGDEA
                     LKIHIHVPTENFYRTQEYAFTLGTVTKIAVENLQEQIAAMQATGARPHGAHQGLTASD
                     IATIAVAPGDGLADVFQSLGASTVISGGQTMNPSTEDFVKAIQALPCERAILIPNNGN
                     VILAARQAAELAGRQVAVVPTRTVPQGIAAMLAFNPQAELEANLHAMEASVQHVRTGE
                     VTIATRAVEMNGVRVRPGQPIGLLDDALAAAGESIEAVVGQLLEKMDAAERELVTIYY
                     GASATAEGAQALADAIRERYSHLEVEVVAGGQPHYPYIFSAE"
     gene            complement(6606..6962)
                     /locus_tag="AC162G_09595"
     CDS             complement(6606..6962)
                     /locus_tag="AC162G_09595"
                     /inference="COORDINATES: protein motif:HMM:NF015721.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR5076110.1"
                     /translation="MGEKTPLGHIEISPTAIASIAARTVTQAYGVVGLASRNFADGIV
                     RTLSGDPHRGVSVTIDGDRIEIDLYVIVEYGTRIASVAASLQNIVRFNVEKALGAPID
                     AVNVHVQGLRVSSTDA"
     gene            7107..7289
                     /gene="rpmB"
                     /locus_tag="AC162G_09600"
     CDS             7107..7289
                     /gene="rpmB"
                     /locus_tag="AC162G_09600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR5076111.1"
                     /translation="MAKCNICGRGPMFGHNDTFSKKKTNRRFDLNVSKRAVVVNGQRR
                     RVWVCNNCLKTLSRSA"
     gene            complement(7341..8024)
                     /gene="rpe"
                     /locus_tag="AC162G_09605"
     CDS             complement(7341..8024)
                     /gene="rpe"
                     /locus_tag="AC162G_09605"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR5076112.1"
                     /translation="MPRPLRIAPSILSADFARLGDQIAEAEAAGADLIHCDVMDGRFV
                     PNLTFGPVVVEAVRRTTRLPIEVHLMIVEPERYLVDYAEAGGDTIIVHAEVSPHLHKT
                     LQEIHRLGGRAGVAINPATPLSAVEEVIDDLDLLLVMTVNPGFGGQELIEGTIAKIAR
                     ARRMLDAAGSDADLEADGGVGAETAGRIAAAGANHLVAGSAVFASGEGIGAAIRRIRH
                     AALESAGVK"
     gene            complement(8085..8990)
                     /locus_tag="AC162G_09610"
     CDS             complement(8085..8990)
                     /locus_tag="AC162G_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR5076113.1"
                     /translation="MLTKHGYVSIAVRAIAVLSAFALIAACQTAPPEPAGEVPPTQTP
                     GERLEARTTSRLISLFSNGDWSAEVQLEVTNGQPPFTYETTWPRLGSDEPATFSVSGS
                     NCEPIVFTAMVTSSDGLERKVQIGIGPVSCEGVTAASPEPSPGATPPTPSGTTTPATT
                     GTPPVTSPPSASLDAATQELLRLINDLRVRNNAPEFAYNDKLAQAAVRHSQNMASTGQ
                     ISLQVTGEPDLAQRVQEAGYTGQSTEIIYAGGADPIDALDWWLTDQTDPGHRTNIIGT
                     EFQEIGIGIVKGAVLTYYTLVFGKP"
BASE COUNT         1557 a         2991 c         2958 g         1771 t
ORIGIN      
        1 cgcagagcca gattgatgcg gtaatcgttc tgcggatcga tgccgatcac cgggtcgggc
       61 gcgacggcgc cgtacgaaac gcccggtatg ggagcgcacc cggatttatg aatggccgga
      121 aggtaaacgt acgatgaact cgcagtcgac cgagcgccgg ctgcggcgtc tccggaggtt
      181 tgtcgtatcg caatcgcagt ggacggcgcg agaagcacga gaatcaagca agcgatcccg
      241 gcgcgccgca gcacgacaaa gctaaagcgc agtgcacaca agatgcgttc cttgtggggc
      301 acgctgctac tttactgaga aacgactaat gtggcaatag gtaatcgggt gcatcatcag
      361 aaccctggtt gctccgagaa accagggttg cgatggctca gtcaagcatg agtggctcaa
      421 ccagaaacct ggtttcttgg ctcagcttac atctccggta ccgctgctcc agaaggcgtg
      481 tagacgctgg gcgagcgcgc catgctccgg cgcttcgagg ttcgggtcgg cggagataag
      541 cttctcggct tcacggcggg ccaggtcgat gagacgcgcg tcggcgatgt tcgcaagccg
      601 catatcaggc aggcccgatt ggcgcgtgcc caggaattcg cccggcccac gcagctccag
      661 atctttctcg gccaacgcga agccgtcctg agtggcttca acggcctgca gccgttcgtt
      721 cattccgttc gccgtcgaat ccgccagcag caggcagtac gacttttccg tgccgcgccc
      781 gacgcgtccc cggaactggt gaagctgtga caggccgaag cggttcgcgc cctcgatcag
      841 catcaccgtc gcgttcggca cgtcgatacc gacttcgacg accgaagtcg agacgaggat
      901 attcaactcg ttcgccgcga aggcgcgcat gacggcgtcc ttctcttcgg atttcatgcg
      961 gccgtgcagc agcccgagct tgaactgcgg gaagatgtgc ttctgcagcc gctcgtgctc
     1021 ttcgaccgcg gctttggctt cgatgttttc cgattcttcg accagcgggc agatgatgta
     1081 cgcctgccgg cccttctcga tctgagatcg gatgaagctg tacatccgtt cgcgctccgt
     1141 ggggaggacg acgcgcgtca cgatgggctg gcggcccggc ggcatctcgt cgatcacgga
     1201 caggtcgaga tcgccgtaca gcgtgagcgc cagcgtgcgc ggaataggcg ttgccgtcat
     1261 caccagcatg tgcggggagg ccggcccctc ttccggcttc acgcctttct gtcgaagcgc
     1321 ggcgcgctgc tgcacgccga aacgatgctg ctcgtcgatc acgacgaaag ccaggtcgcg
     1381 aaactccaca ccctgctgga tcagcgcgtg cgtgccgatc aggatatggg cgtggccgga
     1441 tgcgacgcca gccagcagcg cctcacggtc cgcatgcggc gtgctgcccg ttagaaggtg
     1501 aatcgacacg ggctgcgcca atccaatggc gccgaggttc gcgaatacgc gcgtgaagtt
     1561 ctggtagtgt tgctcggcaa ggatttcggt gggggccatg atcgccgctt gagcgccggc
     1621 ctgcgtcacc gcgaacatcg cggcggctgc cacgatcgtc ttgcccgatc ccacgtcgcc
     1681 ctggatcagg cgattcatcg gctggccgga gcgcatatcg ctcaacacgt cgtcgagcgc
     1741 gcggcgctgg gccgcggtca gcgcataggg cagggcgccg agaaactgat cgagcgtggc
     1801 cggagagacg tcgatggcgc gcccgttctg cgatttccat cggcgccgct gccgaagcaa
     1861 gcccatctgg agcatgaaca gctcgtcgaa ggccaggcgc ttgcgcgcgg catcgagctg
     1921 cgtcgtcgaa tccgggaagt gcgcctgctg aatcgccttg ggcaggctga acagcccggc
     1981 cgacgcgagt accgagtcgg gcaggaaatc gggtatgcgc cccgcccatt cgtcaatagc
     2041 gcgccgcatc agttttcgca ccccgcgtcc cgtcagccct tcggtcagcg ggtagatcgg
     2101 caccaggcgc gccgtgtgca gcaggtcttt ttcgagaagc tcccattccg gagactgaaa
     2161 cgtcagcctt cccaggtact cggtcacctt gccgctgacg acgatttgct ggccgcggcg
     2221 gatcttgtct ttgagataag ggttgaacca cgtgcattga atcgtgcccg atccgtcgct
     2281 gatcacggcc tggacgatct gcgcgccgcc gcgcgtgtgg cgcaccccga cttcccaaac
     2341 ggagccgatg atcgtgacct cttccccgta ttgcagacgg ttgatcggcc ttagcgcggt
     2401 gaagtcgtcg tagcgtcgcg gcaggaagaa cagaaggtcg cgaatcgtct tcacgccgag
     2461 ccggccgagc ttctcggcaa tcgaaggccc gacgcccggg atggccgtcg ccggcgaatc
     2521 gagtgtcgcg cgctcgccac gtgcggggcg gcgatgttcc tgtagaaacg gcgtccccat
     2581 gccgttttgg gaagggcctt ccggcgtaac gctgtcggcg ggccgcggcg ctgcgactgc
     2641 ctccgccggt gcggcgggca ccgcaggccg ccgtgcgaca ggaggaacgg catccccatg
     2701 ccgttcttct tcccgcgtct cgcccgccgt ggtcgtcgcc ggcgagggtg ttgaaggtgg
     2761 ctgggggtgc gcggctgctt gcgaatatgg cgcgccgggc gtgtcggcca cgcctgccgt
     2821 cgccaggctc agcaatgaca gcatgtcctc aaccgactgg cggcgggcct cgatatcgtc
     2881 gatcgctccg tagcgccgca gccgttcggt gatcgcctcg atgcgctggg cgtcgcccga
     2941 ttcgcggcgc gcttcttcct gccactttgg ggcgtactgc gccagcccgc cgatcatcgc
     3001 gcggtcgcgg tagccgaatt cggcctcgag cttaagaatc ttcgtcagct tctcgaaaga
     3061 tgcaggtcgt ccggtcactt tcgtcgctcg tctaccacgc acgccacgcc cagaccgttc
     3121 gggccgacgt acgtgccaat cgccgtcgtc gcctcggcaa cgtagatggg atcactcagc
     3181 gatgagtgca gtgtcctgaa ctcgtcggcc agggcctgtg cggcgttccg tgcgcgagtg
     3241 tgcagcacga tcaggctgcg cagcgctccc tgcccggcga caagctcttt cagacgtgcc
     3301 aagcagtgcg cgcgggtgcg cacgcggtcg acagatagaa cggcgccgtc gcgcacctcc
     3361 acaattggct tgattttgag gagctggccg atcgccgccc tcgcccagcc cacgcgcccg
     3421 ctgcgccgca cgtattcaag cgtgtcgagc gcggcgaaca ggcgcacccg ctgccggacg
     3481 cgggccacgg cgtcgagaat ctcgccgatg ctctggccgg cgcgcgccgc gcgtgcggcc
     3541 tcgatgactt gccagcccag ccccatggac acctggccgg aatccacgat ggccacctcg
     3601 aggtctggca cggcttgcgc tcccatgtgc gccgcgttct gaaccgcgct gagtgtcgcg
     3661 gccacatgca cggagacgac cgcatcggcg ccttcgtcgc gcaggcgacg gtacgtcact
     3721 tcgaattcgc ctgcgcttgg cgaagagctg gtcggcagcg ggtctgccgt gatgagccgc
     3781 gcgtagaact cctcacggct gaggttgact ccgtcgtcgt atgaccgggt gccgaagatc
     3841 acatgcaccg gcacgacgac gatatccagc tcacgcacaa actcttccgg tacgtcggcc
     3901 gtgctatccg tgacaatgcg aaccatgtct cgccctgtgt gtttgagcgg caaacgccgg
     3961 cgcgattacg cgcgtggagc cgcgccttcg taaatgacga tgcccaggct gttaggcccg
     4021 atcaggcagg ccagcgacgc gccgtacatc atcaccggga agtactggcc ggggaatgtt
     4081 tgttccagcc gctcgagcag cagtcgagta tcctctgtcg gctgcgcgcc gctctgcatg
     4141 atggccacgc gttcgatggc ggagaactcg gagacaaact cgacgagctt ctcaatcgcc
     4201 ttctcgcgcg tgcgcacttt ttccatgggg atgatttcgc cgtcctccac ggacaggaac
     4261 ggtttgatgc ccagcatcgc gcccaggatg gactggctct tgctgagacg cttgccgcgc
     4321 tccaggtagt cgagcgaatc cgtgaagtag accgcgtaca tatgcggaat catgccgcgc
     4381 acgatgcgca ccatttcgtc aagcggctct ccgcgcgcgg cagcttcggc ggcggcttcg
     4441 atcagaatgc ccaggcccag cgacgtgtgc atcgagtcga tgaccacgat cttgcctcgg
     4501 cccaggaatt cgtcggccgc ctgccgcgcc cgcgcgatgg tctggttcat ccgcgacgag
     4561 gtgtggatcg agagcacctg ctcgttgcgg cgtatgagcg acgcgtacag cgcgcggaat
     4621 tcgtcgacgg acggcggatg gcactccggc gtgccgatga agtgtggcag ccgccgatag
     4681 aaatcctcgg cgcccaggtc gacgccctcg cggaagtgct gagcgcccaa ttggatgatc
     4741 tgtggcacca ccgtgatccc gagccgctcg accacgccgg ggtcggcgaa gtgcgcgccg
     4801 ctgtcggtga taacatgaac tctggtcaaa ggggcttact ctgcgctaaa gatgtaggga
     4861 tagtgcggct ggcccccggc caccacttca acctcgaggt ggctgtagcg ctcgcggata
     4921 gcgtcggcca gcgcctgcgc gccttcggct gtagccgaag cgccgtagta gatcgtcacg
     4981 agttctcgtt cggcggcgtc catcttttcc aacaactggc cgaccacggc ctcgatcgat
     5041 tcgcccgccg cagccagcgc gtcgtctagc aggccgatgg gctgccccgg cctgaccctg
     5101 acgccgttca tttcgacggc gcgcgtggcg atcgtcactt cgccggtgcg cacgtgctga
     5161 acggatgcct ccatcgcgtg tagattggcc tccagttctg cctgcggatt gaaggccagc
     5221 atggccgcga tgccttgcgg aaccgtgcgc gtcggcacga cggccacctg ccgcccggcc
     5281 agctcggctg cctgacgcgc ggcgaggatc acgttaccgt tgttcgggat caagatcgcg
     5341 cgctcgcacg gcagcgcctg gatcgctttg acgaagtcct ccgtgctcgg gttcatcgtc
     5401 tgcccgccgg atataacggt gctggcgccc aggctctgga agacatccgc aagtccgtcg
     5461 cccggcgcga cggcgatcgt ggcgatgtcg ctggccgtga ggccctgatg cgcgccgtgt
     5521 ggtctcgcgc cagtcgcctg catcgcagct atctgctctt gaaggttctc gaccgcaatc
     5581 ttggtgacgg ttcctagcgt gaacgcgtat tcttgcgtcc ggtagaaatt ctcggtggga
     5641 acgtgaatat ggattttcaa tgcctcgtcg ccactgccga cctggatcga agtgccgaat
     5701 gtcgatagct cctggtgcat ggcgtcccag tcaaggtcgg ggcgcggttt caagtcgaag
     5761 atgagttcgt aatcctgccc ttcttctatg ctctccagca tcgtgctggg agcgaacgca
     5821 agcgtggcga cgatgggttc catctccacc ggctcgcctt ttagatgtcg cagcatgcct
     5881 tcaaagatga cagccagacc ttgcccgccg gcgtcgacta cgccggcctg gcgcaggacc
     5941 tcgagctgcg tcggcgtgcg ggcaaccgct tcctgggcgg caaccagcac ctgttcgaag
     6001 atcagcatca ggtcgtccgt cgtcttggcg gatgcttcgg cagcgtcggc agcggcgcgc
     6061 gagacggaca gaatggtgcc ttctaccggc cggatcacgc cttggtaggc ggtgcggcag
     6121 ccctcgtgca tagccacgac gaagtcggct gcgttcatcg ccggcttgct gcccagtccg
     6181 cgcgcgaaac cccgccacaa ttgcgagagg atgacgcccg agttgccgcg cgcgcccatc
     6241 agcgcgccat gcgcgacggc gtgcgccatg cgatgaattg tcgcgccgtc caccttttcg
     6301 acttctgccc aggccgcctg catcgtgagc agcatgttcg tgccggtatc cccgtcgggc
     6361 acgggaaaga cgttgagcgc gttgacggac tgctggttga cgcgcagcca cgacacgctg
     6421 gcctgcacca tctctttgag ctgcagtcca ctgagtgcat atagaggctg atcgaccgcc
     6481 gatgccaatc cccggctccc aatctgatgt gatactacgc gcctatccga aaaccgtagg
     6541 gcggcatccc catgccgctt gccggcgcat ggggatgcgc ccgctacggg gttttcggat
     6601 ggagtttaag cgtccgtgct gctgacgcgc agaccctgca cgtggacgtt caccgcgtcg
     6661 atcggcgcgc cgagcgcctt ctcgacgttg aagcgaacga tgttttgcag gctcgccgca
     6721 accgacgcga tgcgcgtgcc gtattcgacg atgacgtaca gatcgatctc gatacggtcg
     6781 ccgtcgatcg tcacgctgac tccccgatgc ggatcgcccg acagcgtccg gacaatgccg
     6841 tcggcgaagt tcctgctggc cagcccgaca acaccgtagg cttgcgtcac cgtccgggca
     6901 gcgatgctgg cgatggccgt cggcgagatt tcgatgtggc ccaacggtgt cttctcgccc
     6961 actcaaccct cccaatgaga cttcggaagt ctccggatgt caatgagcct taagacttcc
     7021 gaagtctgag gccagttgac ggaactcttc gcctgtggta acattcgtcc gctctcgaca
     7081 gcgacataat cgtaaggtga tgactcatgg cgaagtgcaa catttgcggc agaggaccga
     7141 tgttcggtca caacgatacg ttctccaaga aaaagacaaa tcgccggttc gatttgaacg
     7201 tcagcaagcg ggccgtcgtc gtcaacggtc agaggcgccg ggtgtgggtc tgtaacaact
     7261 gcctgaagac gctctctcgt tccgcttgaa tctcccgccg ctcccagaag cccggtttct
     7321 gccttcgaga aaccgggttt ttattttacc cctgccgatt cgagcgcagc gtggcgaatg
     7381 cggcggatcg cggcgccgat accttcgcct gacgcgaaca ccgccgatcc tgccaccaga
     7441 tggttggcgc ccgctgcggc aatccggcct gccgtctcgg cgcccacacc cccgtcagct
     7501 tccaggtctg cgtcgcttcc ggccgcgtcg agcattcgtc tggcccgcgc gatcttggcg
     7561 atggtcccct cgatgagttc ctgtccaccg aaccccgggt tcaccgtcat cacgagcagc
     7621 aggtcgaggt catcgatgac ttcctcgact gccgatagcg gcgtcgcggg gttgatcgcc
     7681 acgccggcgc gcccgcctaa ccgatgtatc tcctggagag tcttgtgcaa gtgcgggctg
     7741 acctcggcgt gcacgatgat cgtgtcgccg cccgcctccg cgtagtcgac gagataccgc
     7801 tccggctcga cgatcatcaa gtggacttcg atgggcaatc gcgtcgtgcg tcgaaccgcc
     7861 tctacgacca caggtccgaa ggtgagattc ggcacgaaac ggccgtccat cacgtcacaa
     7921 tggatcaggt cggcgccggc ggcttccgct tcggcaatct gatcgccgag gcgggcaaaa
     7981 tccgccgaga ggatggaagg cgcgattcgc aacgggcgtg gcatcgccgg caattatacc
     8041 cggtcgggag acggatttca agggttatgt catctggcct gctactatgg cttgccgaag
     8101 accagcgtgt aatacgtcag caccgcgcct ttgacgatgc caattccgat ttcctggaat
     8161 tccgtgccga tgatgttcgt ccggtggcct ggatcggtct ggtcggtcag ccaccagtcc
     8221 agcgcgtcga tcgggtcagc gccgcccgcg tagatgatct cagtcgattg accggtatag
     8281 cctgcttctt gcacgcgctg cgccagatcc ggctcgccgg tgacctggag cgagatctgg
     8341 ccggtgcttg ccatgttctg gctgtgccgg actgcggctt gcgccagctt gtcattgtac
     8401 gcaaactccg gcgcgttgtt acgaacgcgc agatcgttga tgagcctgag aagctcctgc
     8461 gtggcagcgt ctagtgaggc ggatggcggt gatgtgacag gcggtgtgcc tgtcgtcgcc
     8521 ggagtggtcg tcccggatgg ggtagggggt gtagcgccgg gcgacggctc cggcgaggcg
     8581 gccgtgacgc cttcgcagga cacaggacct ataccgatct gaaccttgcg ctccaacccg
     8641 tccgacgatg tcaccatcgc ggtgaacacg atcggctcgc agttcgaacc gctgacgctg
     8701 aaagtcgccg gctcgtcgct gcccaggcgc ggccacgtgg tctcgtaggt gaaaggcggc
     8761 tgcccgttcg tgacctcgag ctggacttca gccgaccaat cgccgttgct gaacaggctg
     8821 atcaagcggg acgtggtcct cgcttcgagt ctctcgcccg gcgtctgtgt cgggggcact
     8881 tccccggccg gctccggcgg ggctgtctgg caggcagcga tcaacgcgaa ggccgacaat
     8941 acggcgatgg cccgaacagc aatagacaca tatccatgtt tcgtcagcat gtgacgcgct
     9001 cctttcagat ccccaaatca catcttggcc ggcacggggc gatggttggc gagccacatc
     9061 tggctgttga gcgcctgctg tttcgccccc gctttcgccc actcgtacgt gccgtccttg
     9121 cacattgtga tctcctcgtg cttcggagag caaccctgcg tcagctcagc gcagccgact
     9181 gccgcaactg gccgcagccg gcgtcgatgt cgatgcctcg gcgcacgcgc agggtgttcg
     9241 gaatgccgcg ccgatctagc tcggcgcgaa aggcggc
//
LOCUS       DBNHXB010000234        17267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1062572, whole genome shotgun sequence.
ACCESSION   DBNHXB010000234 DBNHXB010000000
VERSION     DBNHXB010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 17267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17267
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1062572"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1766
                     /gene="hutU"
                     /locus_tag="AC162G_09615"
     CDS             114..1766
                     /gene="hutU"
                     /locus_tag="AC162G_09615"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HIR5076114.1"
                     /translation="MSQRIIRAPRGTQLVCKGWLQEAALRMLMNNLDPDVAEKPDDLI
                     VYGGRGPAARSWESFDAIVAALKELENDETLLVQSGKPVAVFKTHPDAPRVLIANSNI
                     VPHWATQENFDRWERDGLIMFGQMTAGSWIYIGTQGILQGTYETLGSLAGRQGWAEGL
                     KGKFVLTAGLGGMGGAQPLAITMNEGVGLIVEIDPARARRRLETKYVDVVVDDLEEAM
                     TMVEEARNKRQPLSVGLIANAADVFPELVIRGVLPDVVTDQTPAHDPLSYVPHGMSLE
                     AANVLRMHDPDEYTRRSMASMARHVQAMLEFQNKGAVVFDYGNNLRQRAYDAGVKEAF
                     AYPGFVPAYIRPLFCEGKGPFRWVALSGDEKDLHRTDEAILELFPHDKHLERWIRMAQ
                     QRVKFQGLPARICWLGYGERARAGLKFNELVAKGEVSAPIVIGRDHLDAGSVASPRRE
                     TEGMRDGSDAISDWPILNALINAVGGATWVSFHHGGGVGIGYSQHAGQVIVADGTPEA
                     AQRLERVLTTDPGLGVVRHADAGYPEAIAFAKKTGIKMPMLR"
     gene            1873..2280
                     /locus_tag="AC162G_09620"
     CDS             1873..2280
                     /locus_tag="AC162G_09620"
                     /inference="COORDINATES: protein motif:HMM:NF015232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HIR5076115.1"
                     /translation="MPEYRVNQLEAILRTLHESVEGLQGTVVVSLDGFVVAAHLPSGN
                     GRREHTSSAADSPQVAAMAASIIALSDKVLAQLARGSISRVLIDGSQGGIIVVPAGAE
                     AALAVMVSHDAKLGLVMLVLQRSAEQVKRILAR"
     gene            2510..3160
                     /locus_tag="AC162G_09625"
     CDS             2510..3160
                     /locus_tag="AC162G_09625"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR5076116.1"
                     /translation="MKVIIVGCGRVGAELAISVSRSGHDVTVVDQSPTAFDRLGSDFK
                     GRTVQGIAFDRDVLERAGITAADAFAATTSSDNANIIAARIARDIFHVPNVVARIYNP
                     TRREVYERLGLQTVASSTWGAQRIEQLLLHPGLADVEMIGHGEVKLIEMQVPPAWVGQ
                     PANRIMDGQACVIAVTRAGRAFVPKDGDRFQAGDLAYVSLAAELMPQLVESALQVL"
     gene            3178..3843
                     /locus_tag="AC162G_09630"
     CDS             3178..3843
                     /locus_tag="AC162G_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR5076117.1"
                     /translation="MFVLIVGGGKIGSHLATLLLEEGHAVRVIDPRPDVLTKLHRELP
                     AEMIHPGDGTDPARLEQSGIRKAHVLAAVTGDDDTNLVVTTLARFEYGVPRIIARVNN
                     PRNAWLFTPEMGVDVALNQADLMAKMIAEEMSLGDMMTLLKLRKGEVEIVEEKIAPTA
                     IANEKAIKDIQWPEGCTVAAVIRKGEVITPRGDTVLHAYDEVLAVVRTAQRQALATLL
                     GRS"
     gene            3892..5841
                     /locus_tag="AC162G_09635"
     CDS             3892..5841
                     /locus_tag="AC162G_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5076118.1"
                     /translation="MASETSRRFSIADLLIGQPLRTADAPYQAISKKIGLAVFASDAL
                     SSVAYATQEILIVLALAGAATFALSIPISLVIVFLMIVVAVSYRQTIYAYPSGGGAYT
                     VTRENFGRNIAQVAAAALLTDYILTVAVSISSGVEQIASFAPALHPYRVEIALAVIWG
                     MTVVNLRGIKESGRAFALPTYFFVTMMALTLGAGLIQMITGSLGTVTNPPSPSGHEVQ
                     TLTLLLVLRAFASGSTALTGIEAITNGIPAFKEPRSRNAAITLVAMVAILASMFLGVT
                     LLANRVQVFAFEGMKETIISQIARTVFGEGALHVLALVATAVILLMAANTSFADFPRV
                     SSIVAQDGYLPKQLTFRGRRLVFSWGIVALALVASALVAVFNAETTRLIPLYAIGVFL
                     SFTLSQSGMVRRWREIGRLKPGEEQELHGHLVRYDSAWRSKMAVNALGAVMTGTVMLV
                     FAIAKFREGAWIVVILIPTLVYGFVRIHRHYQGVADILSHGSRWPNLRPRPVKTLVLV
                     DDIHAGTIHTINFAKSLGVPWTAVHVAIDPEKAERVKEKWRERIGDEAYLTVLPSPYR
                     NLADPVREYVLKLLEEMPNGFIHVIVGHLVMDKLREQALHQNAAIILNLALQDVDHAT
                     VTTVSYRLETHANGKGRHRAISVKE"
     gene            complement(5887..6219)
                     /locus_tag="AC162G_09640"
     CDS             complement(5887..6219)
                     /locus_tag="AC162G_09640"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR5076119.1"
                     /translation="MRSIPAVRELHHTYQDDGLIVVGVHSPEFNHEKDIDNVRDAIAR
                     LDVPYPVAIDNDFATWRAFDNRYWPTLYLIDERGVIRYSHIGELHKDTKSWNELVSLI
                     EQLRKEPG"
     gene            complement(6229..6507)
                     /locus_tag="AC162G_09645"
     CDS             complement(6229..6507)
                     /locus_tag="AC162G_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076120.1"
                     /translation="MTGLLWSLAASGLILAACAPAAVAPADSAGQSAPAADAPRSSAP
                     EQENAVPNPLPDGPLAPKLKNDVWLNSRPLTTADLRGSVVLIDFWTFG"
     gene            6586..6822
                     /locus_tag="AC162G_09650"
     CDS             6586..6822
                     /locus_tag="AC162G_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HIR5076121.1"
                     /translation="MPIQVELDDVVRLRKPHACGGYEWTVARIGADIGLKCNTCGRRV
                     LLTRRDFEKRLKTFVRRAMAASDSPQTPTSPASS"
     gene            6819..7955
                     /locus_tag="AC162G_09655"
     CDS             6819..7955
                     /locus_tag="AC162G_09655"
                     /inference="COORDINATES: protein motif:HMM:NF018613.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGDG synthase family glycosyltransferase"
                     /protein_id="HIR5076122.1"
                     /translation="MSRRSVRLVFLFADVGGGHRAPAEAVREALRRMAGDAAQVELVD
                     ALADYAPFPYNRGRQTYTQTARFARPMLWLFFHGLDTRQRIRLGVGVMWWPMRRAMRE
                     MIRDHPADLYVSFHPTYSYPLTWLRLPSVFVFTDLVTNHALWCATGAQCYLVPTEVAR
                     RRAIVRGAPAERIAVTGLPVHPRFLDPIEPPDRLRSKLGVALGQPIILLSGGGEGMGR
                     VYDIAHAIGESGLDAQMVVVAGRNQALREELEHADWPMPTRVLGFTRNMPDWMAAASV
                     LIAKSGSNTIAEALVRGLPIILFEFVPGQEEGNPQFVVDAGAGAYCPRPAQVVRTLRD
                     WLNDPARLEELAVNARAASRPNAAFSVARTIVQIAEERARDRSG"
     gene            7939..8565
                     /locus_tag="AC162G_09660"
     CDS             7939..8565
                     /locus_tag="AC162G_09660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR5076123.1"
                     /translation="MIDPDDVRRYYADRRDPAHDFDHVLRVTEMAVRIARAEGADVEV
                     VRAAALLHDVGRGDASGRDHAEVSADVARRILESHPPEKVEAVTQAILQHRFRSGPQP
                     DSLEARCLFDADKLDSIGAIGVARAFAFAGSIGQRLWDEVAPDYAGRFARGDVRPGEH
                     TSHHEYVVKLSRLRERMTTRAGRAIAEERHAAMAAFFERLAREARGEA"
     gene            8585..9637
                     /locus_tag="AC162G_09665"
     CDS             8585..9637
                     /locus_tag="AC162G_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076124.1"
                     /translation="MTRPSKVNLRKLAYGLIALFFFVLAIQLMKAGARGLAPQLEQAG
                     LINSPLNGLGLGWLGAYFVLSGSPVAAAALGLFSGDALDSFQTFMMINGSRMGASFIV
                     LFIGFIYMLRGHEWKRSMSAGLLSMLTTWTTYIPAMALGYALLSNGWLKRFQLSMTGE
                     LVSAIDAVFDPLVAWITSWAPGLLVFVVGVGIIIVSFNLIDRALPELNLGRAGFGEAP
                     RLLYRPLAMFILGMAVTSLSMSVSVSLGLLVPLSARGYIRIENVVPYIMGANVTTFVD
                     TLVVALLMNNPAAFTIVLVQMVSVTIASLAILLVSYRRYERFLLGTAGAITHSRRNQT
                     IFMLAMVGVPIVLLMI"
     gene            9674..10537
                     /locus_tag="AC162G_09670"
     CDS             9674..10537
                     /locus_tag="AC162G_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5076125.1"
                     /translation="MRILMCSDGSATSEQAGQFAAYLLKAPGHQVTLFGAIENEREAG
                     AIKHSLDLLFARLRPARSDVSMKLARGHAAEEILKEAEAGSYDLIIVGARGRRGLTRF
                     LIGSTSSRVVQYAPCSVMIVKKSSRELRRILVCTAGGAPGLRDVRFAARVAAVTESDM
                     TVLHVMSQVALSEDSDLYDLEATAEQLIERGTREGRHLVAALESMRQAGVRGEIRVRH
                     GLVMDEIDAEAREGGYDLVVVGAHFVRGMSRLLLDDVTSHVLEALDAAVLVVRDKPSP
                     PSPSPSPLSQS"
     gene            complement(10564..11331)
                     /locus_tag="AC162G_09675"
     CDS             complement(10564..11331)
                     /locus_tag="AC162G_09675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5076126.1"
                     /translation="MSDYTAFARLYDLFYDDFTQDIAMYRGFAERVGSRVIEIGCGTG
                     RVAIPLARDGCSVTGIDAAEAMLAIARGKAEHAGLADRVKLLQADMRRFDLGARFDLA
                     IAPINTFMHNATLDDQLDTLGCIRRHLAPHGLLVLDLFNPDVHAPNDGRLVLHRTRPG
                     PHGQLILQFTARSLDWENQVSSVMFIVDEPDESGLIRRTTFPFQLRFLFRNEVELLLR
                     QAGFELEAVYGSYDLEPYGEGAEKMIVVARPSLPSPA"
     gene            11591..12802
                     /locus_tag="AC162G_09680"
     CDS             11591..12802
                     /locus_tag="AC162G_09680"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5076127.1"
                     /translation="MVKERILIAEDNKELASFLQSYLLQQGYEAQVAHDGEAAVRLFV
                     AQPFDLVLTDLQMPRLNGLGVLHRVKERSSDAQVIILTAHATLDTALDALRHGAYDFL
                     LKPVDSVDQLQFTINRALLQRSLEIDNRRLLAELKEANAHLGQKVAEQTRELREAYGK
                     LQSLDRMKSEFVSVVSHELRTPLSVILLSTQMLLSGTDSLDADRLHEHLSNVQKYSRR
                     LKRLVDNLLDFSLLQRGELELDIGPVDVAGVIMEVVEVYQPRAEEKGIILRAVAPKTA
                     LTVVGDLARLSNALSHLVDNAVKFTDTGGRVVIGAHGPTQVSGGGNAPYVAIGVADSG
                     VGIPPERQHLLFRAFSQLDMSDSRRFEGIGLGLALANRIVTAHKGRITLKSEPGKGST
                     FAIWLPMEGPR"
     gene            12962..13582
                     /locus_tag="AC162G_09685"
     CDS             12962..13582
                     /locus_tag="AC162G_09685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR5076128.1"
                     /translation="MLIPFSVESLIAFAVVVLAAFPLHEYGHAWMAVRLGDRTPYYQG
                     RLTLDPRAHLDLVGTLLLAVAGFGWAKPVQFVPYNLRNAPSFKVGIVLVALAGPLMNI
                     LIAMVAALLLRIDIGNRTVDGILGAIVLINLLLAVFNMIPLVPLDGSKVLWGLMPDSW
                     SRAYEQIQQYSLFILLILIVPIGGVSLLSRIIFPIVSTLQQAFVGL"
     gene            13579..14088
                     /locus_tag="AC162G_09690"
     CDS             13579..14088
                     /locus_tag="AC162G_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HIR5076129.1"
                     /translation="MKATYRIRQLLAALTARVSEADLAEADACLPPGACSLFRRMPRD
                     GQAHALCVMRDLVRAGHTHSSLLAAALLHDAGKSAARITPLTRSIFVVVERIAPRRLA
                     RWPATGWRRPLAVLRDHPRVGAEWARAAGCDELTVSLIARHQEKLGGAISSEEDRLLK
                     TLQAFDDRN"
     gene            14154..14396
                     /locus_tag="AC162G_09695"
     CDS             14154..14396
                     /locus_tag="AC162G_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR5076130.1"
                     /translation="MPFVYIVECADGTLYTGWAIDVDKRVAAHNAGRASRYTRSRRPV
                     KLVYVEKRVSRAIAMKREAAIKKMPRARKLRLIRDA"
     gene            14485..15747
                     /locus_tag="AC162G_09700"
     CDS             14485..15747
                     /locus_tag="AC162G_09700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5076131.1"
                     /translation="MPYRILMIAPTSFFSDYGCHVRILEEARVLQRHGHAVRIVTYYK
                     GRDLPGLDVVRTRPTPWHADYEVGSSRHKIAFDVLLSWTSLRAALRFKPDIIHAHLHE
                     GALIGSILGAISRAPLVFDFQGSLTGEMVDHHFLNPDGPWHALVRWLERRIDHRPRAI
                     LTSSHAALPLLRDEFGVDPERIIPIPDCVDTTFFRPRAESDAVEVAAIKSYLGVPADR
                     SVVVYLGLLAPYQGTHLLIEAARQVVERRPQTHFLIMGYPGMPQYRAMAEALGLHDHT
                     TFVGKTLYDDAPRYLRLGDVAVAPKLSATEANGKVLNYMATGLPTVAFDTPVNREYMR
                     DYGFYARPGDAASLAGQVLRALDDAPACAGAARGLRARAESAFSWDDAARRITAVYDA
                     VRMRRRSERTRALRALSFEPPRGGWDVG"
     gene            15737..16555
                     /locus_tag="AC162G_09705"
     CDS             15737..16555
                     /locus_tag="AC162G_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076132.1"
                     /translation="MWARLRELWRYRELIRNLVERDLKVRYKNSVLGIAWSWLNPLLM
                     MIVLTVVFTVMAGSSRQPAYHVFVLIGILTWNFFSGSVVGATGSIVNNAHLIKKVYFS
                     RAVLPVSVVLSNMVNFLIALPVFFVLAWLSGVPLSGRIELTLLLPVVILVQILFTIGV
                     GMVLSTVNVFYRDTQIIMEVIMLAWFFLTPIVYPIETVPETATLLGMTIDLRRLVFIL
                     NPMASIVASYRDVLYWGRTIGADFFLRTAVTSIAILLVGYGVFDRYSRRFAEEV"
     gene            16691..>17267
                     /locus_tag="AC162G_09710"
     CDS             16691..>17267
                     /locus_tag="AC162G_09710"
                     /inference="COORDINATES: protein motif:HMM:NF014750.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5076133.1"
                     /translation="MYGYAGQILHVDLTKGALTVEKPDESFYRTYYGGSAMGLYYLLK
                     NTPPKADPLGPENTLSFFVSAPTGVAISGQSRATATAKSPLSGLVGDSQSGGFWPAEL
                     KFAGFDGIVITGKSPRPVYLWIHQGQPELRDASHLWGNGIATGDVEKKIKEEVGEPKA
                     EVIQVGPAAERGVRFANIMNMSNRAFGRTGMG"
BASE COUNT         2974 a         5494 c         5613 g         3186 t
ORIGIN      
        1 agacttcgga agtctcaagg cgtattctcg caggcgacgg tagccggcaa tccgagtagt
       61 ttcgctggcg agatgccact taaagacttc cgaagtctca acggaggcaa gccatgtcgc
      121 aacgcatcat tcgcgccccg cgcggcacgc agttggtatg caagggttgg ctgcaagagg
      181 ccgccctgcg catgctgatg aacaatctcg acccggacgt ggccgagaaa cccgacgacc
      241 tgatcgtgta cggcggacgg ggacccgccg cccggtcgtg ggagtcattc gacgccatcg
      301 tcgccgcgct gaaggaactg gaaaacgacg agacgctcct ggtgcaatcg ggcaagccgg
      361 tggccgtgtt caagacgcac cctgacgcgc cgcgcgtgct gatcgccaac tcgaacatcg
      421 tgccgcactg ggcgacacag gagaacttcg atcggtggga gcgcgacggt ctgatcatgt
      481 tcggccagat gacggccggc tcgtggatct acatcggcac gcagggtatc ctgcaaggca
      541 cgtacgagac gctcggctcg ctcgcgggca ggcaaggctg ggccgaaggg cttaagggca
      601 agttcgtgct gacggccgga ctgggcggca tgggcggcgc gcagccgctc gcaatcacga
      661 tgaacgaagg cgtcggcctg atcgtcgaga tcgacccggc gcgagcgagg cgcaggctgg
      721 agacgaagta cgtcgacgtg gtcgtggacg acctggaaga agcgatgacg atggtcgaag
      781 aggcaagaaa caagaggcaa ccgctgtcgg ttgggctgat tgcgaacgcc gccgacgtgt
      841 tccccgagct ggtgatacgg ggcgtgctgc ccgacgtcgt caccgatcag acgcccgcgc
      901 acgatcccct ctcctacgtc ccacacggaa tgtcgctcga agcggcgaat gtgctgcgga
      961 tgcacgatcc cgacgaatac acacgccgct cgatggcgtc gatggcgcgc cacgtgcagg
     1021 ccatgctcga gtttcagaac aagggcgccg tcgtgttcga ctatggcaac aacctgcgcc
     1081 agcgcgcata cgacgcaggc gtgaaggagg cattcgccta cccgggtttc gtgccggctt
     1141 acatccggcc cctgttctgc gaaggcaaag gccccttccg ctgggtcgcg ctgagcggcg
     1201 acgagaagga cctgcatcgc acggatgaag ccatcctgga actgttcccg cacgacaagc
     1261 acctcgagcg gtggatccgc atggcgcagc agcgcgtgaa gttccagggc ctgcctgcgc
     1321 gcatctgttg gctgggttac ggcgaacggg ccagagccgg gttgaagttc aacgagctgg
     1381 tcgcgaaagg cgaggtatcc gcgccgatcg tcatcgggcg cgatcatctc gacgccggct
     1441 cggtcgccag cccgcgccgc gagacggaag gcatgcgcga cgggtcagac gccatcagcg
     1501 actggccgat cctcaacgcg ctgatcaacg ccgtcggcgg cgcgacgtgg gtgagcttcc
     1561 accacggcgg cggcgtcggc atcggctaca gccagcacgc gggccaggtg atcgtcgccg
     1621 atgggacgcc cgaggcggcg cagcggctgg agcgggtgct caccaccgat ccgggcctgg
     1681 gcgtcgtacg acacgcggac gccgggtatc ccgaagcgat cgcgtttgcg aagaagaccg
     1741 gaatcaagat gccgatgcta cgataagcga gtcggcgaat cggcgagtca gcgatgctgt
     1801 tcgccggctc tccgattcac caagcgtggt atactgtaga tattatcatg atctgacaga
     1861 ccggataggt gaatgcctga gtatcgcgtc aaccagctcg aagccatact gcgcactctg
     1921 cacgaatcgg tcgagggatt gcagggcacg gtcgtcgtca gcctcgacgg gttcgtcgtc
     1981 gcagcgcacc tgccttccgg caacggccgt cgcgagcaca cgtcgagcgc agccgacagc
     2041 ccacaggtcg ccgccatggc ggcctcgatc atcgccttga gtgacaaagt cctggcgcag
     2101 ttggcgcgcg gctcgatcag ccgcgtcctg atcgacggca gccagggcgg catcatcgtc
     2161 gtgccggccg gcgccgaagc ggccctggca gtgatggtca gccacgacgc gaagctcggc
     2221 ctggtgatgc tggtgctgca gcgctcggcc gagcaggtga aacgcatact cgcgcggtag
     2281 cgcttcatct ttgacacagc gtggtgtcct cggtataatc tcgcctgttt agggtcagac
     2341 gagcgtttca ctcgcgtagt tcctgtcggg aaacgccgta gaacggtatc ccgatgccgt
     2401 tcggataagg ccggcatagg gatgccaacc tactttcccg acaagaacta gcgtaacgcc
     2461 cgcccgtcca ggggcgggtt tttatttgcg agtttgtgga gaaaactgta tgaaagtcat
     2521 catcgtcgga tgcggccgcg tcggcgccga gctggccatc tcggtatcgc gcagcggcca
     2581 cgacgtgacc gtggtcgatc aaagcccgac cgcgttcgat cggctaggct ccgacttcaa
     2641 agggcgcacg gtgcagggca tcgcgttcga ccgcgacgtg ctggagcgcg cggggatcac
     2701 ggcggcggac gccttcgccg cgaccacgtc gagcgacaat gcgaacatca tcgccgcccg
     2761 gatcgcccgc gacatcttcc acgtcccgaa cgtcgtcgcg cgcatttaca atccgacgcg
     2821 gcgcgaggtg tacgagcgac tgggcctgca gaccgtcgct tcatcgacgt ggggcgcgca
     2881 gcgcatcgag cagttgctgc tccatccggg cctggccgac gtcgagatga tcggccacgg
     2941 cgaagtcaaa ttgatcgaga tgcaggtccc ccccgcgtgg gtcggccagc cggcgaaccg
     3001 gatcatggat ggccaggcgt gcgtgatcgc cgtcactcgc gccgggcgcg cgttcgtgcc
     3061 caaggacggc gaccggttcc aggcaggcga tctggcatac gtcagcttgg cggcggaatt
     3121 gatgccgcag ttggtcgaat cggcgctgca ggtcctataa cgatgggaga agaggtcatg
     3181 ttcgtgttaa ttgtcggcgg cggcaagatc ggctcgcact tggctacgct gctgcttgaa
     3241 gagggccacg ccgttcgcgt gatcgatccg cgtccggacg tcctgacgaa gctgcatcgc
     3301 gagctgccgg ccgagatgat ccatcccggc gacggcaccg atccggcgcg gctggagcag
     3361 tcgggcattc gcaaggctca cgtcctggcg gcggtgaccg gcgacgacga caccaacctg
     3421 gtcgtcacta ccctggcgcg cttcgaatat ggcgtgccgc gcatcatcgc ccgcgtgaac
     3481 aatccgcgca acgcctggct gttcacgccg gaaatgggcg tggacgtggc gctcaaccag
     3541 gccgatctga tggcgaagat gatcgccgag gagatgtcgc tgggcgacat gatgacgctg
     3601 ctcaagctgc gcaagggcga ggtggaaatc gtcgaggaga agatcgcgcc gacagcgatt
     3661 gccaacgaaa aggccatcaa agatatccag tggccggaag gctgcacagt ggcggccgtc
     3721 atccgcaagg gcgaagtgat cacaccgcgt ggcgacacgg tgctgcacgc gtacgacgaa
     3781 gtgctggccg tcgtgcgcac cgcacagcgc caggcgctgg ccacgctgct cgggcgatcg
     3841 taggcgattc caggtgaaac cggatcgttc cacgtcagaa cggagtgatt tatggcatct
     3901 gaaaccagtc ggcgtttctc gatagccgac ctcttgatcg gccagccgct gcgcacggcc
     3961 gacgcgccgt atcaggctat cagcaagaag atcggcctgg ctgtcttcgc ctccgacgcg
     4021 ctttcgtcgg tggcgtacgc cacgcaggaa atcctgatcg tgctggcgct ggccggcgcc
     4081 gccaccttcg cgttgtcgat ccctatttcg ctggtcatcg tcttcctgat gatcgtcgtc
     4141 gccgtctcgt accggcagac gatctacgcg tatcccagtg gcggcggcgc gtacacggtg
     4201 acgcgtgaga atttcggccg gaacatcgcg caggtggccg ccgccgcctt gctgacggat
     4261 tacatcctga cggtggcggt cagcatttcg agcggcgtcg agcagattgc gtcgttcgcg
     4321 ccggcgctgc atccgtaccg cgtcgagatt gcgctggctg tgatctgggg catgacggtg
     4381 gtcaacctgc gcggcatcaa ggaatccggc cgagcgttcg ccttgccgac gtatttcttc
     4441 gtcacgatga tggcgttgac gctgggggcg ggattgattc agatgatcac tggcagcctg
     4501 gggactgtca cgaatccacc gtcgccgtcc gggcacgaag ttcagacgct gacgctgctt
     4561 cttgtactgc gcgcgttcgc cagcggctcg accgcgctga ccggcatcga agccatcacg
     4621 aacggcatcc cggcgttcaa ggagccgcgc agccgcaacg cggcgatcac actcgtcgcg
     4681 atggtggcga tcctggcgtc gatgttcctg ggcgtcacac tgctggcgaa ccgcgtgcaa
     4741 gtgtttgcct tcgaaggcat gaaggaaacg atcatctctc aaatcgcgcg cacagtgttt
     4801 ggcgaaggcg cactgcatgt gctggcgctc gtcgccacgg ccgtcatcct gttaatggcc
     4861 gccaatacct cgttcgccga ttttccgcgc gtaagcagca tcgtggcgca ggatggttac
     4921 ttgccgaagc agcttacttt ccgcgggcgg cgcctggtgt tttcgtgggg catcgtggcg
     4981 ctggccctgg tcgcgtctgc actggttgcc gtcttcaacg ccgagacgac gcgcctgatc
     5041 ccgctgtatg ccatcggcgt gttcctgtcg ttcaccctgt cgcagtccgg catggtcagg
     5101 cgttggcgcg agatcggcag gctgaagccg ggcgaggagc aagagctgca cggccacctg
     5161 gtgcgatacg actctgcctg gcgatcgaag atggcggtca acgcgttggg cgcggtgatg
     5221 accggcaccg tgatgctggt cttcgccatc gccaagttcc gcgagggagc atggattgtg
     5281 gttatcctga tcccgacgct cgtctacgga ttcgtgcgca tccatcggca ctatcagggt
     5341 gtggccgaca tcttgagcca cggctcgcgc tggccgaatc tgcgtccacg gccggtgaag
     5401 acgctggtac tggtggacga cattcacgcc ggaacgatcc acaccatcaa cttcgccaaa
     5461 tcgctgggtg tgccctggac ggcagtgcac gtggccatcg atccggagaa ggccgagcgg
     5521 gtgaaggaga agtggcgaga gcgcatcgga gacgaggcgt atctcaccgt cctgccctca
     5581 ccatatcgca acctggccga tccggttcgc gagtacgtgc tgaaactgct ggaagaaatg
     5641 ccgaatggtt ttatccacgt catcgtcggc cacctggtga tggacaaact ccgcgagcag
     5701 gcgctccacc agaacgcggc gatcatcttg aacctggccc tgcaggacgt cgatcacgcg
     5761 acggtgacca cggtttcgta tcgcctggag acacacgcca acggcaaggg ccggcaccgc
     5821 gccatctcgg tgaaggaata aaagaagacc tggcaggtct cgcagacctg ccaggtctac
     5881 aacaactcac cccggttctt tccgcaattg ctcgatcagg ctcacgagct cgttccagct
     5941 tttcgtatcc ttgtgcaact cgccgatgtg gctgtagcgg atcacgccgc gctcgtcgat
     6001 gagatacagc gtcggccaat aacggttgtc gaacgcgcgc cacgtagcga agtcgttgtc
     6061 gatcgccacc gggtagggca cgtccaggcg ggcgatggcg tcccggacgt tgtcgatgtc
     6121 cttctcgtgg ttgaactccg gcgaatgcac gccgacgacg atcaagccat cgtcctggta
     6181 cgtgtggtga agctcccgca ccgcgggaat cgagcgcacg cagttgattc agccgaacgt
     6241 ccagaaatcg atcagcacga cgctgccgcg caggtcagca gtggtgagcg gcctgctgtt
     6301 gagccacacg tcgtttttca atttgggcgc gagaggcccg tcgggtaacg gattcggcac
     6361 ggcgttctcc tgttcgggcg cggatgatct gggagcgtct gccgccggcg cagattgccc
     6421 ggcggaatcg gccggggcca cggcggctgg cgcgcaggcg gcgagtatca ggcccgacgc
     6481 ggcgagactc cacagcaatc ctgtcatctc agtattcctc cctctgcgcc tgcgagtgta
     6541 gccaccagat attacagcct acttacgaca tggtataatc gcgccgtgcc catacaagtt
     6601 gaactcgacg acgtggttcg gctgcgcaag ccccacgcgt gcggcgggta cgagtggacg
     6661 gtcgcgcgta ttggcgcgga catcggcctg aaatgcaaca cgtgcggtcg gcgcgtgctc
     6721 ctcacccggc gcgacttcga gaagcgcctc aagaccttcg tgcggcgcgc gatggccgcc
     6781 agcgattcgc cgcagacccc tacttcacca gcgtcatcgt gagccggcga tctgttcgac
     6841 tcgtatttct gtttgccgat gtcggcggcg ggcatcgcgc gccggccgaa gccgtgcgcg
     6901 aggcgttgcg gcgcatggcc ggcgatgcgg ctcaggtgga gttggtcgac gcactcgccg
     6961 actacgcgcc gttcccttac aaccgcgggc ggcagaccta cacccagacg gcgcgcttcg
     7021 cacgtcccat gctgtggctg ttcttccacg ggctggatac tcgccagcgc attcgtctgg
     7081 gcgtgggcgt gatgtggtgg cccatgcgcc gggccatgcg cgagatgatc cgagatcatc
     7141 ccgccgacct gtacgtctcc tttcacccga cgtacagcta tccgctcacc tggctgcgcc
     7201 tgcccagcgt gttcgtgttc accgatctgg tgacgaatca cgcgctatgg tgcgcgactg
     7261 gcgcgcagtg ttacctggtg ccgaccgagg tcgctcgccg acgggcgatc gtgcgcgggg
     7321 cgcccgccga gcgcatcgcc gtgaccggct tgccggttca tccccgcttt ctcgatccga
     7381 tcgagccgcc cgaccggctg cgaagcaaac tcggcgtcgc cctgggccag ccgatcattc
     7441 ttttgagcgg cggcggcgag gggatgggtc gagtgtacga cattgcgcat gcgatcggcg
     7501 aaagcggtct ggacgcgcag atggttgtcg tggccgggcg caaccaggcg ctgcgagagg
     7561 aattggagca cgccgactgg cccatgccga cgcgcgtgct cggctttacc cgcaacatgc
     7621 cggactggat ggcggcggca tccgtgctga tcgccaagtc cggctcgaat acgatcgccg
     7681 aagcgctggt gcgcggcctg cccatcatcc tgtttgaatt tgtgcccgga caggaggagg
     7741 gcaacccgca gttcgtcgtc gacgccgggg cgggcgctta ctgtcctcgc ccggcgcaag
     7801 tcgtccgcac gctgcgcgac tggctgaacg atccggcccg tctggaggag cttgccgtga
     7861 acgcgcgcgc cgcgtctcgg ccgaatgcgg cattcagcgt tgctcgcacc atagtccaga
     7921 tcgccgagga gcgcgcccgt gatcgatccg gatgatgtcc gacgctatta cgccgaccgg
     7981 cgcgatcccg cgcacgattt cgaccacgtg ctgcgcgtga cggagatggc cgtgcgtatt
     8041 gcccgcgccg aaggcgccga cgtcgaggtg gtgcgcgccg ccgcgctgct gcacgacgta
     8101 gggcgcggcg atgcgtcggg acgcgatcac gccgaggtct ccgccgacgt tgcgcgccgc
     8161 atcctcgaat ctcatccgcc ggagaaggtc gaagccgtca cgcaggcgat cctccagcat
     8221 cgcttccgct ccggtccaca gcccgactcg ctcgaagcgc gctgcctgtt cgacgccgac
     8281 aagctcgatt cgatcggggc gatcggcgtg gcgcgtgcgt tcgccttcgc cggctccatc
     8341 ggccagcggc tgtgggacga ggttgcgccc gactacgccg gccgcttcgc gcgcggcgac
     8401 gtccggccgg gcgagcatac ctcgcatcac gagtacgtcg tgaagctgtc gcggctgcgc
     8461 gagcggatga cgacgcgggc aggccgcgcc atcgccgaag agcgacatgc cgccatggcc
     8521 gcgttcttcg agcgcctggc gcgtgaggcg cgcggcgagg cttaaaccct cgcccattcc
     8581 ctctatgaca cgcccgagca aggtcaacct gcgaaaactc gcctacggcc tgatcgcgct
     8641 ttttttcttc gtcctggcga ttcagttgat gaaggccggt gcgcgggggc tggcgccgca
     8701 gttggagcag gccggcctga tcaacagccc gctcaacggt ctgggcctgg gctggctcgg
     8761 cgcctacttc gtgctcagcg gatcgccggt cgcagcggcc gcgctgggcc tgttcagcgg
     8821 cgacgcgctc gactcgttcc agacgttcat gatgatcaac ggctcgcgca tgggcgcgtc
     8881 gttcatcgtg ctgttcatcg gcttcatcta catgctgcgc ggccacgagt ggaagcgcag
     8941 catgagcgcg ggactgcttt cgatgctgac gacgtggacg acgtacattc cggctatggc
     9001 gctggggtac gccttgctgt cgaacggctg gctgaagcgg ttccagttgt cgatgaccgg
     9061 ggagctggtg tcggccatcg acgctgtatt cgatccgctc gttgcctgga tcacctcctg
     9121 ggcgcccggc ctgttggtct tcgtcgtcgg cgtgggcatc atcatcgtgt ccttcaacct
     9181 gatcgatcgc gcgctgccgg agctaaacct gggacgcgcc gggttcggcg aagcgccgcg
     9241 cctgttgtat cgccctctcg ccatgttcat cctgggcatg gccgtgacct cgctgtcgat
     9301 gtcggtgtcg gtttcgctcg gactgctggt gccgctctca gcgcgcggct acatccgcat
     9361 cgaaaacgtc gtgccctaca tcatgggcgc gaacgtgacc accttcgtcg acacgctggt
     9421 ggtcgcactg ctgatgaaca acccggcggc tttcaccatc gtgctggtgc aaatggtgag
     9481 cgtgacgatc gcatcgctcg ccatcctgct cgtgtcgtat cgacgctacg agcgcttcct
     9541 gctgggcacg gccggcgcga tcacgcacag ccgtcgcaac cagaccatct tcatgctggc
     9601 gatggtcggc gtgcccatcg tgcttctcat gatttgacag gcgactgatg ctcagacagt
     9661 cggagtgtgc aaagtgcgga ttctgatgtg ctcagacggc tcggcgactt ccgagcaggc
     9721 ggggcagttt gccgcctacc tgctgaaagc gccgggccac caggtgacat tgttcggcgc
     9781 gatcgagaac gagcgcgaag ccggcgcgat caagcactcg ctcgacctgc tcttcgcgcg
     9841 cctcaggccc gcccggtcag acgtgtcgat gaagctggcg cgcggccacg ccgccgagga
     9901 gattctcaaa gaggcagagg cggggagtta cgacctgatt atcgtcggcg cccgtggccg
     9961 gcgcggactg acgcgttttc tgatcggttc gacctcgtcg cgcgtcgtgc agtatgcgcc
    10021 ctgctcggtg atgatcgtca agaagtcgtc gcgcgagctg cgccgcatcc tggtgtgcac
    10081 ggccggtggc gcgccgggcc tgcgcgacgt gcgcttcgcg gcgcgcgtcg ccgccgtgac
    10141 cgagtccgat atgaccgtgc tgcacgtgat gtcacaggtg gcgctgagcg aagactccga
    10201 tctatacgat ctggaggcca cggccgagca gttgatcgag cgcggcacgc gcgagggccg
    10261 gcacctcgtg gccgcgctcg aatcgatgcg gcaagccggc gtgcgcggcg agatccgggt
    10321 gcgccacggc ctggtgatgg acgaaatcga cgccgaagct cgcgaaggcg gctacgacct
    10381 cgtcgtcgtc ggcgcccatt tcgtgcgcgg catgagccgt ctcctgctcg acgacgtcac
    10441 gtcgcacgtg ctggaggcgc tggatgcggc ggtgctggtc gtgcgggata agccctcacc
    10501 cccctccccc agcccctccc ccctctccca atcctagatt gggagagggg ggaggggtgg
    10561 ccgtcaggcc ggggagggga gtgaaggccg cgccacgacg atcatcttct ccgccccttc
    10621 tccgtatggc tccaagtcgt atgacccgta cacggcttcc aactcgaagc cggcctgtcg
    10681 cagcagcagc tcgacttcgt tacgaaacag gaagcgcaac tggaagggga aggtcgtgcg
    10741 gcggatcagc ccgctctcgt caggctcgtc gacgatgaac atgaccgagg agacctggtt
    10801 ttcccagtcg agcgagcggg ctgtgaactg caagatgagc tgcccgtgcg ggccgggccg
    10861 cgtgcggtgc agtaccagac ggccatcatt cggcgcgtgc acatcgggat tgaacaggtc
    10921 gaggacgagc aggccatgcg gtgcgagatg ccgccggatg catcctagcg tgtcgagctg
    10981 atcgtcgagc gtcgcgttgt gcatgaacgt attgatcggc gcaatcgcca gatcgaagcg
    11041 cgcgccgagg tcgaagcggc gcatgtcggc ctgcagcagc ttcacgcgat cggcgaggcc
    11101 ggcgtgctcc gccttgcccc gcgcgatcgc cagcatcgct tcggcggcgt cgatgccggt
    11161 gacggaacag ccgtcccgag caagcggaat cgccacgcgg ccggtgccgc agccgatctc
    11221 gatcacgcgg ctacccactc gctcagcgaa gccccgatac atcgcgatgt cttgtgtgaa
    11281 atcgtcgtaa aagagatcgt acagacgcgc gaatgcggtg taatcgctca tcatgccagc
    11341 ctgatccgaa cggcatcccg atgccgttct acggcgtttc tcaacaggaa cgagtctcag
    11401 tagatccaaa ttcggaagca gagcgggctc ccacgcccgc gaatcagcgg catgggagcc
    11461 gcctccgctg gcaattttag atctattgag tctagcacgg cgagcgacct ggggcaagtt
    11521 gatctgtgcg tcgcctgcgc tataatccat gcaggcccac aggagaggat cgaagacctg
    11581 gagggtgtgg atggtcaaag agcgcatcct gattgccgag gacaacaaag agcttgcctc
    11641 ctttctccaa tcgtatctgc tgcagcaggg ctacgaggcg caggtggccc acgacggcga
    11701 ggcggccgtg cgcctgttcg tggcgcagcc gttcgacctg gtgctgacgg atctgcagat
    11761 gccgcgtctg aacggcctgg gcgtgctgca tcgggtgaag gagcgttcca gcgacgcgca
    11821 ggtcatcatc ctcaccgcgc acgccacact ggacacagcg ctggatgcgc tgcgccacgg
    11881 cgcgtacgat ttcctgctca agccggttga cagcgtcgac cagcttcagt tcacgatcaa
    11941 ccgtgcactc ctgcagcgca gcctggagat cgacaaccgc cgcctgctcg ccgagctgaa
    12001 ggaggccaac gcgcatctgg gacagaaagt ggcggagcag acgcgcgagc tgcgcgaggc
    12061 gtacgggaag ctgcaaagcc tggatcgcat gaagagcgag ttcgtgtcgg tcgtgtcgca
    12121 cgagctgcgc acgccgctgt ctgtcatctt gttgtcgacg cagatgctcc tctccgggac
    12181 tgattcgctc gacgccgatc gcctgcacga gcacctgagc aatgttcaga aatatagccg
    12241 ccgcctgaag cgcttggtcg acaatctgct cgacttcagc ctgttgcagc gcggcgagct
    12301 ggagctggat attggccctg tcgacgtggc cggcgtgatc atggaagtcg tcgaagtgta
    12361 tcagccgcgc gcggaggaga agggcattat cctgcgggcc gtggcgccca agacggcgct
    12421 gaccgtcgtc ggcgacttag cccgattgtc caacgcgctg agccatctgg tcgacaatgc
    12481 ggtcaagttt accgacacag gcgggcgtgt ggtgatcggc gcgcacggcc cgacgcaggt
    12541 gtccggcggg gggaacgccc catacgtcgc catcggcgtc gccgattcgg gagtcggcat
    12601 tccgcccgag cgacagcatc ttttgttcag agccttctcg cagctcgaca tgagcgatag
    12661 ccgtcgattc gagggtatcg gtctgggcct ggcgctggcg aaccgcatcg tcaccgcgca
    12721 caagggccgc atcacgctga agagcgagcc gggcaaaggc agcacgtttg ccatctggct
    12781 gccgatggaa gggccgcgct agccgcccaa atttcacagc cgccatgtgt gacgcacttc
    12841 ccaagtgcgt cgcatatcgt ctccctcaca gcccgcgtcc tcgcgggcgt tgtccaccct
    12901 gtcccctttt tgatgtgtta taatggcatc ccgcattcct tgtatctaag ctcagaaacc
    12961 tatgctcatc ccgttttccg ttgagagcct gatcgcattt gccgtcgtgg tgctcgccgc
    13021 gtttcccttg cacgagtacg ggcacgcctg gatggccgtc cggctaggcg atcggacgcc
    13081 gtactatcag ggccgcctga cgctcgatcc gcgtgcgcac ctcgacctgg tcggcacgct
    13141 cctgttggcc gtcgccggct tcggctgggc caagccggtg cagttcgtgc cgtacaatct
    13201 gcgcaacgcg ccgagcttca aggtcggcat cgttttggtc gcactggccg gcccactgat
    13261 gaatattctg atcgcgatgg tcgcggcgct cctgctccgt atcgacatcg gtaaccggac
    13321 cgtcgacgga atcttggggg cgattgtcct catcaatctg ctgctggccg tgttcaacat
    13381 gatcccgctc gtgccgctcg acggctccaa agtgctgtgg ggcttgatgc ccgattcctg
    13441 gtcgcgtgcg tacgagcaga ttcaacagta cagcctgttt atcctgctga tcctgatagt
    13501 gccgataggc ggcgtgagcc tgttgagccg gatcatcttt ccgatcgtgt cgacgctgca
    13561 gcaggcgttc gtcggacttt gaaagcgacg taccgcattc gacagttgct ggccgcgctc
    13621 accgcgcgcg tgtctgaagc cgatctggcc gaggcggatg cctgcttgcc gcccggcgcg
    13681 tgcagcctgt tccggcgcat gccccgcgat gggcaggcgc atgcgctgtg tgtgatgcgc
    13741 gatctggtgc gcgccggaca tacgcactcg tcgctgctgg cggcggcgct gctgcacgac
    13801 gccggcaaat cggcggcgcg catcacgccg ctcacgcgct cgatattcgt cgtggtcgag
    13861 cgcattgcgc cgcgccggct cgcgcgctgg ccggcgacgg gctggcgcag gccgctcgcc
    13921 gtccttcgcg atcatccgcg cgtcggggcc gagtgggcgc gcgcggccgg ctgcgacgag
    13981 ctgaccgtgt cgctcattgc gcggcatcaa gagaagctag gcggcgctat atcgagcgaa
    14041 gaagatcgtt tgctgaagac gcttcaagcg ttcgacgatc gcaattgatc gacgactcga
    14101 ccatccgacc attcgaccat tcgaccactt gaccattcga ctatccaacc accatgcctt
    14161 tcgtttacat tgtcgagtgc gcggatggaa cactgtatac cggctgggca atagacgtgg
    14221 acaagcgcgt cgccgcacac aacgcaggac gcgcatcacg ctacacgcgc tcgcgccggc
    14281 ccgtgaagct ggtctacgtc gagaagcgtg tgtcgcgcgc catcgctatg aagcgtgagg
    14341 ccgcgatcaa gaagatgccc cgcgccagga aactacgatt gattcgtgat gcatgatcat
    14401 gcaaccgtga cggttaacca attaccgatc actaattact gatcgccgat tactgatcac
    14461 tgatcactga ccactgaccc agccatgccc taccgtatct tgatgatcgc ccctacgtcg
    14521 ttcttttccg actacgggtg ccacgttcgc atcctcgaag aggcgcgcgt gctgcagcgc
    14581 cacggtcacg ccgtacggat cgtgacctat tacaaaggcc gcgacctgcc cggcctggac
    14641 gtcgtccgca cgcgacccac gccctggcat gctgactacg aagtcggctc gtcgcggcac
    14701 aagatcgcgt tcgacgtgct gctctcgtgg acgtcgctcc gcgcggcgct gcgcttcaag
    14761 ccggacatca tccacgcgca tctgcacgag ggcgctctga tcggatcgat cctgggcgcg
    14821 atcagccgcg ccccgctggt gttcgatttc cagggaagcc tgaccggcga aatggtcgat
    14881 caccactttc tcaacccgga cggcccgtgg catgcgctcg tgcgctggct ggagcgccgc
    14941 atcgaccacc ggccccgcgc gatcctaacc agctcgcacg cggcgctgcc cttgctgcgc
    15001 gacgagttcg gcgtcgatcc cgagcgcatc atccccatac ctgactgcgt cgacacgacg
    15061 ttcttccgcc cgcgtgcaga gagcgacgca gtagaggtgg ccgcgatcaa gtcttacctg
    15121 ggcgtgcccg cagatcgttc ggtggtggtg tacctcggcc tgctagcacc gtaccagggc
    15181 acgcatctgt tgatcgaagc cgcgcgccag gtcgtggagc ggcgccccca gacccacttc
    15241 ctgatcatgg gataccccgg catgcctcag tatcgagcga tggccgaggc gctcggcctg
    15301 cacgatcata cgacgttcgt cggcaaaacg ctgtacgacg acgcgccgcg ctacttgagg
    15361 ctgggcgatg tcgccgtcgc gccgaaactc tcggcgacgg aggcgaatgg caaggtcttg
    15421 aactacatgg cgaccggact gccgaccgtg gccttcgaca cgccggtgaa tcgcgaatac
    15481 atgcgcgatt acggcttcta cgcgcggccg ggcgacgcgg cctctctcgc cgggcaagtg
    15541 ctgcgcgcgc tggacgatgc gcccgcgtgc gcgggggcgg cacgcgggct gcgcgcgcgc
    15601 gccgagtcgg cgttctcgtg ggacgacgcc gcgcgccgca tcacggccgt atacgacgcc
    15661 gtgcgcatgc gccgccggag cgagcggacg cgggcgctgc gcgcgctgag cttcgagccg
    15721 ccacgcggag gctgggatgt gggctagatt gcgcgagctg tggcgctacc gcgagctgat
    15781 tcgcaacctc gtcgagcgcg atctcaaggt gcgctacaag aactccgtgc tgggcatcgc
    15841 ctggtcgtgg ctcaatccgc tcctgatgat gatcgtgctg acggtcgtct tcacggtgat
    15901 ggccggcagc agccgccagc ccgcctatca cgtcttcgtc ctgattggca tcctgacctg
    15961 gaacttcttc tcggggtcgg tcgtcggcgc gacgggcagc atcgtcaaca acgcgcattt
    16021 gatcaagaag gtatacttct cgcgcgccgt gctgcccgtg tccgtcgtgc tgtcgaacat
    16081 ggtcaacttc ctgatcgcct tgccggtctt cttcgtgctc gcctggcttt ccggcgtgcc
    16141 gctcagcggc cggatcgagc tgacgctcct cctgccggtc gtgattttgg tgcagatcct
    16201 ctttaccatc ggcgtcggca tggtgctgtc gaccgtcaac gtgttctatc gcgacacgca
    16261 gatcatcatg gaagtgatca tgctggcgtg gttcttcctc actccgatcg tctatccgat
    16321 cgagacggtg ccggagacgg cgacgctcct cggcatgacc atcgatctgc gccggctggt
    16381 gttcatcctc aacccgatgg cgtcgatcgt cgcctcgtac cgcgacgtgc tgtactgggg
    16441 acgcacgatc ggcgcggact tcttcctgcg cacggccgtc acgtcgatcg cgatcttgct
    16501 cgtcggctac ggcgtgttcg acaggtattc acggcggttt gcagaggaag tctagagtta
    16561 gagttagagt cgcactctaa ctcccgactc taactctaac tccaactcct cactctaact
    16621 ctttacttgg cattaacttc ggtttatact agcatcgcgc actcgacatc tcatcgtgag
    16681 gagagacatc atgtacggtt acgctggtca aatcctgcac gtcgatctga cgaaaggcgc
    16741 actgaccgtc gagaagccgg acgagtcgtt ctatcggacg tactacggcg gcagtgcgat
    16801 ggggctgtat tacctgctca aaaacacgcc gcccaaagcc gatcccctcg ggccggaaaa
    16861 cacgctgtcg ttcttcgtca gtgcgccgac tggtgtagcc atctccggcc agagccgcgc
    16921 gacggccact gccaaaagcc cgctcagcgg actggtcggc gattcccagt cgggtgggtt
    16981 ctggccggcc gagctgaaat tcgccggctt cgacggcatc gtcatcaccg gcaaatcgcc
    17041 caggccagtc tacctgtgga ttcatcaggg tcagccggag ctgcgcgacg cgagccatct
    17101 gtggggcaac ggtatagcca cgggcgacgt cgagaaaaag atcaaagagg aggtcggcga
    17161 gccgaaggcc gaggtgattc aggtcggtcc ggccgccgag cgcggcgtgc gcttcgccaa
    17221 catcatgaac atgagcaacc gcgccttcgg ccgcacgggc atggggc
//
LOCUS       DBNHXB010000235        11336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3244356, whole genome shotgun sequence.
ACCESSION   DBNHXB010000235 DBNHXB010000000
VERSION     DBNHXB010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11336
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3244356"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1865)
                     /locus_tag="AC162G_09715"
     CDS             complement(<1..1865)
                     /locus_tag="AC162G_09715"
                     /inference="COORDINATES: protein motif:HMM:NF046269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HIR5076134.1"
                     /translation="MESRLTHDPASACQVDLRADTGYNGAAPAAARAPTYLSERMPLD
                     FAALLKDRIDLSRIPFSDRSARIMFFRGDRPPEPGDRPANFFTIRLAERWEKQQAQVG
                     HYRQRRPVIEGFVFIDANGNPLPFELTTYPHEVIFDTPAGRVRMCFADTETLFFTLPP
                     GPIGMRFRVHAASGRVDRRGGVFRGTRNVAYTTNATLVSNEIASIDDSTLQAAFVAES
                     AGGAGLLLNITPRLGFNRTMPVPAAALDAAAARWQAWFAAAPPVEARYQRQYYYAWWI
                     MRAGLISPRYYMTREGMTPSKIHYVGVWQWDAFFHALAYRHVDARLAEDQVRLVFDHQ
                     RDDGMLPDAVHDEGLVTRLSHPVPGEVTKPPLAAWTVWRLYEKSGNREFLEEVYEPLV
                     RWHDWWFEHSDDDRDGLCQYNHPFSSGLDDSPLWDGGMPVESPDLNTYLCLQQESLAR
                     IAGAIGLHDDARVWAHRADAMAQRLIDKMWDEEAGVFWALRDKQPIRILTPFSLYPLW
                     TGRLPQHITARLIEHLTDPDEFWTRYPLPTVARSDPHYDPVQMWRGPTWVNVNYLFIE
                     GLQRCGRNDLAAELRDRTLDLVMQRDDIFEDDHPETGDHPPKAAPIFGWTSAVFI"
     gene            2059..2565
                     /locus_tag="AC162G_09720"
     CDS             2059..2565
                     /locus_tag="AC162G_09720"
                     /inference="COORDINATES: protein motif:HMM:NF019533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HIR5076135.1"
                     /translation="MTELETFRKEKDKFFARDPHSPLTPEQKKNFHGLDYFPENPALR
                     LEAPIEEFPQKEQIVMQTSTGDTQTYVRYGRFKFSVDGQPAELTIYASPHGFFLPFVD
                     ALAGKETYGAGRYLDPETLPNGKFLIDFNLAYNPYCAYNENWSCPLTPFENRLKVPIR
                     AGEKIFHE"
     gene            2637..3308
                     /locus_tag="AC162G_09725"
     CDS             2637..3308
                     /locus_tag="AC162G_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5076136.1"
                     /translation="MATDSIRVILVDDHAVVRKGIREFLTESADIEVVAEASDGDEAI
                     ALIEQHRPDVAVLDIQMPKRSGIDVCRHVRAQQWPVGVLILTAFDDDPYVMAALQAGA
                     NGYVLKTADADDIIQAVHDVHEGKSALDPAVARKLMAQLTGRRSDRPIESLTPRELDV
                     LRLAARGYTNKAIGAQLSISDRTVQGHLANVFDKLHVATRTEAVMRAVSLGWIPPALS
                     ELDQE"
     gene            3348..5069
                     /locus_tag="AC162G_09730"
     CDS             3348..5069
                     /locus_tag="AC162G_09730"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5076137.1"
                     /translation="MAGSSKTRQLLSGLRLRGLTLQLFVFIILPLAALLIAIPLGSLT
                     VHQRAMRGLVGERDERAARATADSITEQINHRAAAVRGLALRAADSVSPEQVVADYAF
                     LLPDFEGGLALFGPDGDVLAASNTPGAWPSRPVADLLARARSQTEPPFSDVFIDAVSS
                     EPMMLVASAAADGTAAVGGFFPASLARRVLTHAFPPDSQVSVLIVDSQARLLYQWDDS
                     LRETDPAQHPGVAEALRGESGTLYRQVEGSEHVVAFSPVPPVGWALVIEEPWEIVYSP
                     LLRTTLAAPLILIPVLLFALVALGFGIRQIVQPLRALEQKTTQLAGGQFDAVESPVGG
                     IAEIRSLQDSLIQMARKLKTAQQRLRDYAAAILRSQEDERSRLARELHDESVQALVAL
                     NQRVQMAERALARDPQAAPARLAELRTMTAAALEDVRRVIRALRPIYLDDLGLLPALE
                     TLTQTIGREGGLITTFEVEGESRRLTPERELALYRIVQEALHNVARHAKAKHVQVKLR
                     YDHALCVSVADDGIGFDVPERVDAMVDLAHFGLVGMRERAEVVGARLEIRSAPGSGTT
                     VELYLPL"
     gene            5266..5490
                     /locus_tag="AC162G_09735"
     CDS             5266..5490
                     /locus_tag="AC162G_09735"
                     /inference="COORDINATES: protein motif:HMM:NF016811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HIR5076138.1"
                     /translation="MKKKSASRLVTDPVCNMELPASEAAATLKFNGRTYYFCHVACQR
                     LFEADPLAYITDVQVGNDTTPLGKAGWDSG"
     gene            5552..5749
                     /locus_tag="AC162G_09740"
     CDS             5552..5749
                     /locus_tag="AC162G_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076139.1"
                     /translation="MFKWLDTHFDQMALTIVVWLCSLPLIVLIVLPLFGLQVAVVAAL
                     VLLFVMLAVCWGICAWKTVKG"
     gene            5760..6824
                     /locus_tag="AC162G_09745"
     CDS             5760..6824
                     /locus_tag="AC162G_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076140.1"
                     /translation="MIGSVPATHSTRQASLAGEQQVVARLVQAAAEAGVRADPVSIVN
                     VYVALKSTPLVILTGPARSGKIALVHSLGHVLAGNDPFRCQIMHGHAWWAEQCRDVAF
                     FNEMQTRLNADKILALIDEAWQPENTKCVFMACLTRISRAELTGFFSEVAFQLRHGQI
                     MRLPSAHLTEPIPYPPNLFLIGTMDDIRPNGLDAGLLSGASVIPWPAAETRSATGRTQ
                     TVPIPGGESLFLHSCIRNAVAARLKLDYILGRRTHVIRPLLLVAELFQQHAVPMSDSV
                     IGKSIIYLANAWSNEGVGLFDRVSSHNFVIATDLAIAQTILPSTGEAIRRSAALHGQL
                     KAALNGRFPRASALLDSLNE"
     gene            6872..7072
                     /locus_tag="AC162G_09750"
     CDS             6872..7072
                     /locus_tag="AC162G_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076141.1"
                     /translation="MQVHRAGRCKKNRSVWPGLFLAGVILVVTIASVARIGWIALAPF
                     GFAALILAILLWLMRATDRAAK"
     gene            7121..7291
                     /locus_tag="AC162G_09755"
     CDS             7121..7291
                     /locus_tag="AC162G_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011849410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HIR5076142.1"
                     /translation="MVKDLVCGMEVDPKTAAGESEYKGQTYYFCSPGCKKAFDKDPEK
                     YVHALHEHSGHH"
     gene            7363..8151
                     /gene="lgt"
                     /locus_tag="AC162G_09760"
     CDS             7363..8151
                     /gene="lgt"
                     /locus_tag="AC162G_09760"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR5076143.1"
                     /translation="MITISIDPVIFGIGHIVIRWYSVIVTLAIAVGTGIVWREAKRRG
                     LAEKHFSDAILWVIIAGLIGARLFHVLDHWTHEYAAYPIRALYIWEGGLAIWGALIGG
                     GIAVAVFAWLRKLPLGRLFDIAAPGLVLGQAIGRVACVITGDAMGKPTTGPFGFAYTN
                     PGAMVPELGVYYIPMPVYEMIANLSIFALLWQLRKRNWPNGLLFLVYLSLYSVERFLL
                     AFTSSYQIIALGLTQSQIVALISLAVALPLMARMRARAHPTYQI"
     gene            8178..8426
                     /locus_tag="AC162G_09765"
     CDS             8178..8426
                     /locus_tag="AC162G_09765"
                     /inference="COORDINATES: protein motif:HMM:NF023095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HIR5076144.1"
                     /translation="MKTKHLLIMLACCLVPLAALAAIAVFNLPANTVITFGILLLCPL
                     LHFAMMRNMGHPHDEHDGHHLTAKTPQLTRGEDKELTR"
     gene            8589..9317
                     /locus_tag="AC162G_09770"
     CDS             8589..9317
                     /locus_tag="AC162G_09770"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076145.1"
                     /translation="MAQHSLIEIHDLKKVYTRGRETVEALRGVSLDIAPGEFVSVMGP
                     SGCGKSTLLHVIGGIDRPTGGSVRVNGADLAAASEDELTRFRRDHVGFVFQFYNLLPT
                     LSASENVELPLVALKYPRLSRRKMARAALGLVGLADRATHRPAELSGGQQQRVAIARA
                     LVSNPKVILADEPTGDLDTASAHMVVDIMRELNRDLGLTFVVVTHNPEVGAAGSRIIH
                     LRDGQVVGDERVSPDSHAAGGRAS"
     gene            9363..10538
                     /locus_tag="AC162G_09775"
     CDS             9363..10538
                     /locus_tag="AC162G_09775"
                     /inference="COORDINATES: protein motif:HMM:NF052764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR5076146.1"
                     /translation="MKKRIIFGVLIGVLIIGAWLGYSRLRAEAVDAAVATPVAAGSDI
                     ALGVSASGKVVPARWAQLSVQIGGPIEMLVEEGQFVEAGHIVARLDGAELQHAVHEAR
                     AARALAEADLARLKAGARSEDIASAEAAVRAAQAELTAAVAGRDRLNKGAQAADVAAA
                     EAALSETEANLKIAQDTYDRIAMYGTVEEQMRAQLAAARQARDAAQARLNQLLAGAGD
                     ELKAASAGVALALAQRDAAQAAFDKAQAGATREEIAAAEARVEMARAAVERAQVTLDR
                     ATLGAPFAGTIARVLVRTGELVGSGQPVLVLGDLSRWQIETDDLSEEDVARVSAGQPV
                     AITFDALPNLTLHGRVTRIAPMATPGQGGTNYTIVIAVDEADARLRWGMTAYPAFED"
     gene            10541..>11336
                     /locus_tag="AC162G_09780"
     CDS             10541..>11336
                     /locus_tag="AC162G_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076147.1"
                     /translation="MNPFRFSHLAWNDLRYGRWRALLMIAGVGVVVAVFFTLSSIAGG
                     LGSVVSATPASERNLVLIDKGATDYCQGQIPEAIVETLRRYPGVAGVVPMLHTALRWK
                     GQMVLIRAIEPEEYGGMAGLRIMEGKLSKRANSVIAGEQLARQNGWRVGDTLEIGGQE
                     LKLTGIFQAGGTLNSELWTSLEDGQRLLSRGDSYSAIVVQAAPDADVRTLQNRMAGAT
                     VLARETDIDTEPEVYETMNRSFGQVTRVMNAISALALIAIAFGVYNV"
BASE COUNT         2052 a         3532 c         3690 g         2062 t
ORIGIN      
        1 tcgataaaca cggccgaggt ccagccgaag atcggcgcgg ccttcggcgg atgatcgccc
       61 gtctcgggat gatcgtcttc gaagatgtcg tcgcgctgca tgaccaggtc gagcgtgcgg
      121 tcgcgcagct cggctgccag atcattccgc ccgcagcgct gcaagccttc gataaacagg
      181 tagttgacgt tcacccacgt tggcccgcgc cacatctgaa ccggatcata gtgtggatcg
      241 gatctggcga cggtcggtag tggatagcgt gtccagaact cgtccggatc ggtcaaatgc
      301 tcaatcaatc gcgccgtgat gtgctgcggc aggcgtcccg tccacagcgg gtatagactg
      361 aacggcgtaa ggatgcgaat cggctgcttg tctcgtaacg cccagaacac gccggcctcc
      421 tcgtcccaca tcttgtcgat caggcgctgc gccatcgcgt cggcgcggtg cgcccacacg
      481 cgcgcgtcgt catgcaggcc gatcgcgccg gcgatgcgcg cgagcgattc ctgctgcagg
      541 cacaggtacg tgttgagatc gggcgactcg accggcatcc cgccatccca cagcgggctg
      601 tcgtcgaggc cggacgagaa aggatggttg tactggcaca gcccgtcgcg atcgtcgtcg
      661 ctatgctcga accaccagtc atgccagcgc accagcggct cgtacacttc ttcgagaaac
      721 tcgcgattgc ccgacttctc atacagtcgc cacacggtcc aggcggcgag aggcggcttg
      781 gtgacctcac cgggcaccgg atggctgagg cgcgtgacga ggccctcgtc gtgcacggca
      841 tcggggagca tgccatcgtc gcgctggtgg tcgaacacca gccgcacctg atcctcggca
      901 agtctcgcgt cgacgtggcg atacgcgagc gcgtggaaga aggcgtccca ctgccacacg
      961 ccgacgtagt gaatcttgct gggcgtcatg ccttcgcgcg tcatgtagta gcgcgggctg
     1021 atcagcccgg cgcgcatgat ccaccaggcg tagtagtatt ggcgctggta gcgcgcctcg
     1081 accggcggcg cagcagcgaa ccacgcttgc caacgggcag cggcggcgtc gagggcggca
     1141 gccggaacgg gcatcgtgcg gttgaagccg agacgcggcg taatgttcag caacaagcct
     1201 gcgccgcctg cactctcggc gacgaaggcg gcttgcagtg tcgagtcgtc gatcgaagcg
     1261 atctcgttgc tcaccagcgt cgcattcgtc gtgtaagcca cgttgcgtgt gccgcgaaac
     1321 acgccaccac gccgatcgac ccgtccgctc gccgcatgaa cacgaaagcg catcccgatc
     1381 gggccgggcg gcagtgtgaa aaatagcgtc tcggtgtcgg cgaagcacat ccgaacccgg
     1441 ccggcaggcg tatcgaagat cacttcgtgc ggataggtgg tcagctcgaa cggcagtgga
     1501 ttcccgttcg cgtcgatgaa aacgaatccc tcgatgaccg gccggcgctg gcgataatgc
     1561 ccgacctgcg cctgctgctt ctcccagcgc tcggccaggc ggatggtgaa gaagttggcg
     1621 ggccgatcgc caggctcggg gggcctgtcg ccacggaaaa acatgatgcg cgcgctgcga
     1681 tcgctgaacg gaatgcgcga caggtcgata cgatctttga gaagagcggc gaaatccaaa
     1741 ggcattctct ccgacagata ggttggtgcg cgagcggctg ccggcgcagc gccgttatac
     1801 cccgtatccg ctctgagatc aacctggcag gcgctggccg ggtcgtgcgt aaggcggctt
     1861 tccatagccg ccatcttaga gactgtctta agacccccgt gttaagaccc gcgtgctaag
     1921 acaccgtggt tgacgcatcc ctatgcgcca acagcgggca taattgggta gcccgccgcc
     1981 aatcgagata caattgagcg agccatggag cacggctccc aggtgagaga ggaatcaaac
     2041 tggtggatga ggcaaatcat gaccgaactg gagacattcc gcaaagagaa ggacaagttt
     2101 ttcgcgagag acccgcacag cccgctgacg ccggagcaaa agaagaactt ccacggcctc
     2161 gactacttcc cggagaatcc tgcgctccgc cttgaggcgc ccatcgagga attcccccaa
     2221 aaggaacaga tcgtcatgca gacctcgacg ggcgacacgc agacatacgt ccgctacggg
     2281 cgcttcaagt tcagcgtaga cgggcagccc gccgagctga cgatctacgc cagcccgcac
     2341 ggcttcttcc tgcccttcgt cgacgcgctc gccgggaagg agacctacgg cgcagggcgt
     2401 tatctcgatc cggagacgct cccgaacggg aagttcctga tcgacttcaa cctggcctac
     2461 aacccgtatt gcgcgtacaa cgagaactgg tcgtgcccct tgaccccttt cgagaaccgg
     2521 ctgaaggtgc cgattcgggc gggggagaag atcttccacg aatgagcagc gaagtagctc
     2581 aggtgcgtga agtgtccccg ccctcgcaag cgtttgtcca gtgacttgag gttgacatgg
     2641 ctacagactc cattcgcgtc atcctcgtcg acgatcacgc ggtcgtgcgc aaaggcatcc
     2701 gcgagttctt gactgaatcg gccgatatcg aggtcgtcgc cgaagcgtcg gacggcgatg
     2761 aggctatcgc gctgatcgag cagcaccggc cggacgtggc cgtgctagat attcagatgc
     2821 ccaagcgaag cggcatcgac gtgtgccgcc acgtgcgcgc gcagcagtgg ccggtcggcg
     2881 tgctgatcct gaccgctttc gacgacgatc cctacgtcat ggccgccctg caggccggcg
     2941 cgaacggcta cgtgctcaag acggcggacg cggacgacat catccaagca gtacacgacg
     3001 tgcacgaagg caagtcggcg ctcgatccgg ccgtcgcgcg caaactgatg gcgcaactca
     3061 ccggtcgcag atccgaccgg cccatcgaat cgctcacgcc acgcgagctg gacgtattga
     3121 ggctggccgc gcgcgggtat acgaacaagg ccatcggcgc tcagctcagc atcagcgacc
     3181 gcacggtgca aggccacctg gcgaacgtct tcgacaagct gcacgtcgcc acccgcaccg
     3241 aggccgtcat gcgcgcggtc tcactcggct ggatcccgcc tgcgttgagc gagttggacc
     3301 aggaataacc tctgcttccc gatgcttctg gagaatcgca ccagcctatg gccggctctt
     3361 cgaaaactcg gcaactgctt tcgggtctgc gcctgcgcgg cttgacgctt caactcttcg
     3421 tgttcatcat cctgccgctg gcggcgctgc tgattgccat cccactcggt agtttgacgg
     3481 tgcaccagcg cgccatgcgc ggcctggtgg gggagcgaga cgagcgcgcg gcgcgagcca
     3541 ccgccgattc gatcaccgag cagatcaatc accgcgccgc agccgtacgc ggtttggcac
     3601 tgcgcgccgc cgattccgtc tcgcccgaac aagtcgtggc cgactacgcc tttcttttgc
     3661 cggactttga aggcgggctg gcgttatttg gccccgatgg cgatgtgctc gcggcttcga
     3721 acacgcccgg cgcctggcca tcgcggcccg tcgccgatct cctggctcga gcgcggagcc
     3781 agactgagcc acccttctcg gatgtcttca tcgacgccgt gagcagtgag ccgatgatgc
     3841 tggtcgcctc tgctgcggcg gatggcacgg ctgcggtcgg cggcttcttc ccggccagcc
     3901 tcgcccgtcg cgtcctgacc catgcgtttc cgcctgacag tcaggtgtcg gtcctgatcg
     3961 tggatagcca ggcgcgcctg ctctatcagt gggacgattc gctacgagaa accgaccccg
     4021 cccaacatcc cggcgtcgcc gaagcgctgc gcggcgagag cggcacgctc tatcgacagg
     4081 tcgaggggag cgagcacgtc gtggcgttca gtccggtgcc gcccgtcggt tgggcgttgg
     4141 tgatcgagga gccttgggag atcgtctata gcccgctgct gcgaacgacg ctggcggctc
     4201 ctctcattct gattcctgtg ctgctcttcg cgctggtggc gctgggattt ggcatccggc
     4261 agatcgttca gcccctgcgg gcgctggagc agaagacgac tcaactggcc ggagggcaat
     4321 tcgacgccgt cgagtcgccg gtgggcggca tcgccgagat tcgcagcctg caagacagcc
     4381 tgatccagat ggctcggaag ctgaagacgg cccagcaacg cctgcgcgat tatgccgccg
     4441 ccatcctgcg cagccaggaa gacgagcgat cgcgcctggc gcgcgagttg cacgacgagt
     4501 cggtgcaggc gctggttgcg ctgaatcagc gtgtgcagat ggccgaacga gcgctggcgc
     4561 gcgatccaca ggccgccccg gcccggctgg ccgagttgcg cacgatgacg gccgcggcgc
     4621 tcgaagatgt ccggcgcgtc attcgggcgc ttcgcccgat ctacctggac gatctcgggc
     4681 tgctgccggc actggagacg ttaactcaaa ccatcggccg cgagggcgga ctgatcacga
     4741 ccttcgaggt cgagggcgag tcgcgccggc tcacgccaga gcgcgagttg gcgctctacc
     4801 gtatcgtgca ggaggcgctt cacaacgtcg cccggcatgc gaaggccaaa cacgtgcagg
     4861 tcaagttgag atacgaccac gcgctgtgcg tgtcggtcgc cgacgacggg atcgggttcg
     4921 acgtgcccga gcgggtcgat gcgatggtcg acctcgcgca tttcgggctg gtcggcatgc
     4981 gcgaacgcgc cgaggtcgtt ggtgctcgtc ttgagattcg atctgcgccg ggcagcggca
     5041 cgaccgtcga actctacctg ccactgtagc tgcgctcctg cgccgcaagc ccgccattat
     5101 cggcgggctt ttcgttttcc ggcgaattcg cctgtgctca cccccgccaa acagcgtggg
     5161 gcaaaacggg ccgtttggcg ctggaatcgg cggaggggtg agcgtatagt tgctcgtagg
     5221 gcggccgccg ggttcgtatt cattcgcctc cacaggtgcg tcaatatgaa aaagaaatcc
     5281 gcatccagac tcgtcaccga tccggtgtgc aacatggagc tgccagccag cgaggctgcg
     5341 gccacgttga agttcaacgg ccggacgtat tacttctgcc acgtcgcttg ccagcggctg
     5401 ttcgaggctg atccgctggc atacatcacc gatgttcagg tagggaacga cacaacgccc
     5461 ttgggcaagg ccggatggga ttctggttga tgcgcagcca gtgaaagggc gtctgtgagt
     5521 ggcccggcag gtgttcgagc gaggaggcgc gatgttcaag tggctcgata ctcacttcga
     5581 ccagatggcg ctgaccatcg tcgtgtggct gtgcagtctg ccgctgatcg tgctcatcgt
     5641 ccttcctctg tttggtctac aggtggccgt ggtcgccgcg ctggtgttgt tgttcgtcat
     5701 gctggccgtg tgctggggca tctgcgcctg gaagaccgtt aagggctgaa gaggtgagtg
     5761 tgatcggcag tgtaccggcg acccattcca cccggcaagc gtcgctcgcc ggtgaacagc
     5821 aagtcgtcgc ccgcctggtt caggcggccg ccgaggccgg tgtacgggcc gacccggttt
     5881 caatcgtcaa cgtctacgtg gcgctcaagt ccacaccact ggtgatcctg accggcccgg
     5941 cacggtctgg aaagatcgcg ctggtgcaca gcctcgggca cgtgctggcg ggcaacgatc
     6001 ctttccgctg ccagattatg cacggccacg cctggtgggc ggaacagtgc cgcgatgtcg
     6061 cctttttcaa cgagatgcag acacggctca atgccgacaa aatcctcgcg ctcatcgacg
     6121 aggcctggca gccggagaac acgaagtgtg tcttcatggc ctgcctgact cgcatcagcc
     6181 gggcggaact gacaggcttt ttctccgagg tggcgtttca acttcggcac gggcaaatca
     6241 tgcgtctgcc gagcgctcac ctgactgaac cgatcccgta tccaccaaac ttgtttctca
     6301 tcggaacgat ggacgacatt cgcccgaatg gtctggacgc cggcttgctc tccggagcaa
     6361 gtgtcattcc ctggccggca gcagagacca gatcggcgac cggccgtact caaacagtgc
     6421 cgatcccagg cggcgaaagc ctattcctac attcatgcat tcgcaatgcg gtggccgccc
     6481 gcctgaaact cgattacatc ctgggccgtc gaacgcacgt catccggcca ctgcttctgg
     6541 tggcagaatt gtttcagcaa catgccgtgc cgatgtcgga ctcggtgata ggcaaatcca
     6601 tcatctatct tgccaacgcg tggtccaacg agggggtcgg acttttcgat cgggtatctt
     6661 cccacaactt tgtgatcgcc accgacctgg cgattgccca gacgattctg ccgtctacgg
     6721 gagaggcgat acgacgctcg gcagcgctgc atggccagtt gaaagcggcg ttgaatggcc
     6781 ggttcccacg cgcgtcggcc cttctggaca gtctcaacga ataatagggc agaggcaagt
     6841 cgatggccag caaagcaagt gagacggtag gatgcaagta catagagcag gaaggtgcaa
     6901 gaagaatcgc tcggtctggc ccggcttgtt cttggctggc gtgatactgg tcgtcacgat
     6961 agccagcgtg gctcgaatcg gctggatcgc gctcgccccg ttcggctttg ctgcgcttat
     7021 tctcgccatc ctgctgtggc ttatgcgcgc gacggatcgc gctgcgaaat gatcgtctat
     7081 ccgtgtgtta attctatcga tgaaaggagg tgaggctcac atggtcaagg atctggtctg
     7141 cggcatggaa gtcgatccga agacagccgc cggcgaatcc gagtacaagg ggcagaccta
     7201 ctacttctgc tctcccggct gcaagaaggc gttcgacaag gacccggaga agtacgtcca
     7261 cgcgttgcac gagcacagcg gccatcacta ggcgacttac cgcggggcgg ctgagccgct
     7321 gccggtcgcc cctgcgactc tcatttcgga aaggttcggt ggatgattac catcagcatc
     7381 gatcccgtca tcttcggcat cggtcacatc gtgattcggt ggtatagcgt catcgtgaca
     7441 ctggccatcg ccgttggcac gggaatcgtc tggcgtgagg cgaagcgtcg cggacttgcc
     7501 gagaagcatt tcagcgacgc catactctgg gtgatcatcg ccggactgat cggcgcgcgg
     7561 ctctttcacg tgctcgatca ctggacgcac gagtatgctg cgtacccaat tcgggccttg
     7621 tacatctggg agggcggtct ggcgatttgg ggcgcgctga tcggcggagg aatcgccgtc
     7681 gccgtattcg cgtggctgcg caaattgccg ttggggcgat tgttcgacat cgccgcgccg
     7741 ggcctcgtgc tggggcaagc catcgggcgc gtggcatgcg tcatcaccgg cgatgcgatg
     7801 ggcaagccga cgaccggccc gttcggcttc gcctacacca atcccggcgc gatggtgccg
     7861 gagttgggtg tgtactacat tcccatgccg gtgtacgaga tgatcgcgaa cctgagcatc
     7921 ttcgcgctgc tgtggcagtt gcgcaagcgc aactggccca acgggctgtt gttcctggtc
     7981 tatctaagcc tatacagcgt tgagcgcttc ttgctcgcgt tcaccagttc gtatcagatc
     8041 atcgcgttgg ggctgacgca atcgcagatc gttgcgctca tctcgctggc cgtcgcgttg
     8101 cccctcatgg cgagaatgcg ggcacgcgca caccccacgt atcaaatctg agcgcccaca
     8161 tacatggagt ttcctgaatg aagaccaaac acctgctcat tatgctcgcc tgctgcctgg
     8221 ttccgctggc agcactggcc gccatcgccg tgttcaatct tccggccaac acggtgatca
     8281 ccttcggcat cctgctgttg tgcccgctgc tgcacttcgc gatgatgcgc aacatgggcc
     8341 acccccatga tgaacacgat gggcaccacc tgacggcgaa gacaccgcag ttgaccaggg
     8401 gcgaggacaa ggagttgacc cggtaactgc gcctgcagga ttgaccttcc tcataggtag
     8461 tttcgcctat cccgcaacgc gccactttgc gcttatctcc aatattccca cgtgctatcc
     8521 tacaactgat gatgtttctg gccaccgcgc cctttgcgcg cggaaagatg accggcacag
     8581 gaggcggtat ggcgcagcac agcctgatcg aaatccacga cctcaaaaag gtgtacaccc
     8641 gcggccgcga gaccgtcgag gcgctgcgcg gcgtcagcct cgacatcgcg ccgggcgagt
     8701 tcgtcagcgt gatggggccg tcgggctgcg gcaagtccac gctcctacac gtgatcggcg
     8761 ggatcgaccg gcccaccggc ggatccgtgc gtgtgaacgg tgccgacctc gccgcagcga
     8821 gcgaggacga gctgacgcgc ttccggcgcg atcacgtcgg cttcgttttc cagttctaca
     8881 acctgctgcc cacgttgtcg gcgtcggaga atgtcgagtt gccgctggtc gcgttgaagt
     8941 atccccggct gtcccgccgc aagatggcgc gcgccgcgct cggcctggtt gggctggccg
     9001 accgcgcgac gcaccgcccc gccgagctgt cgggtgggca acaacagcgc gtcgccattg
     9061 cccgcgcgct ggtctccaat cccaaagtga ttctggccga cgagccgacc ggcgacctgg
     9121 acacggcctc agcgcacatg gttgtggaca tcatgcgcga actcaatcgc gatctgggtt
     9181 tgaccttcgt cgtcgtgacg cacaacccgg aggtgggcgc ggccggctcg cgcatcatcc
     9241 atttgcgcga tgggcaggtg gtcggagacg aaagggtaag ccctgactcg cacgcggcgg
     9301 gtggccgggc gagttgaagt cgttcggtca agaccgagcg tgcatagcag gaggatgcaa
     9361 ctatgaaaaa gcgcatcatt ttcggtgtac tgatcggcgt gttgatcatc ggggcatggt
     9421 tgggctacag ccggttgcgc gccgaggcag ttgacgccgc cgtggccacg cccgtggcgg
     9481 ccgggtcaga catagcactc ggcgtgtctg cttcggggaa ggtggtcccg gcgcgctggg
     9541 cgcagttgag cgtccagatc ggcggcccga tcgagatgct ggtcgaagaa ggacagtttg
     9601 tcgaagccgg tcacatagtc gctcggttgg acggcgcgga gttgcagcac gccgttcacg
     9661 aagcgcgcgc ggcacgggcg ctggccgaag cggatctcgc gcggctgaag gccggcgcgc
     9721 gcagcgagga tatcgcttct gcggaagctg cggtgcgcgc agcgcaggcc gaactgaccg
     9781 ccgccgtcgc cggccgcgat cggctcaaca agggtgccca ggcagcggac gtggccgccg
     9841 cggaagccgc tttgagcgag accgaggcca atctcaagat cgcgcaagac acctacgacc
     9901 ggattgcgat gtacggcacg gtcgaggaac agatgcgcgc gcaactggcc gctgcgcggc
     9961 aggcccgcga cgcagcccag gcgcggttga accaacttct ggccggggct ggcgacgaac
    10021 ttaaggcagc cagtgcaggc gtggctctgg cgctggcgca gcgcgacgct gcacaggcgg
    10081 cattcgacaa ggcacaagcg ggcgcgacgc gtgaagagat cgcggcggcc gaagcgcgcg
    10141 tggagatggc acgcgcggcg gtagaacgcg cgcaagtcac gctggatcgc gccacgctcg
    10201 gcgcgccgtt cgctggcacg atcgcacggg tactcgtgcg caccggcgag ctggttgggt
    10261 cgggccagcc ggttttagta ctcggagact tatcgcgctg gcagatcgag accgacgatc
    10321 tgagcgaaga agatgtcgcg cgcgtctccg ccggccaacc ggtcgccatc accttcgacg
    10381 cactgcctaa cctgacgctg cacggccgcg tgacgcgcat cgcgccgatg gcaacgcccg
    10441 gccagggcgg gacgaactat accatcgtca ttgcggtcga cgaggcggac gccaggttgc
    10501 gctggggcat gaccgcctat cccgccttcg aggactgacg atgaacccgt ttcgcttctc
    10561 gcaccttgcc tggaacgacc tgcggtatgg ccgatggcgc gcgctgctga tgatcgccgg
    10621 cgtgggcgtg gtggtggctg tgttcttcac gctatcttcg attgccggcg ggttgggcag
    10681 cgtggtctcg gccaccccgg cctcagagcg caacctggtg ctcatcgaca aaggcgcgac
    10741 ggattactgc cagggccaga ttccagaggc gatcgtggaa acgctgcggc gctacccggg
    10801 cgtggcaggc gtcgtgccga tgctgcacac cgccttgcgc tggaaagggc agatggtgtt
    10861 gatccgcgcg attgagccgg aagaatacgg cgggatggcc ggcctgcgta tcatggaggg
    10921 caagttgtcg aagcgagcga actcggtcat cgccggcgag caactggcgc ggcagaatgg
    10981 ctggcgggtg ggcgacacgc tggagatcgg cgggcaggaa ctgaaactca ccggcatctt
    11041 tcaggccggc ggcacgctga actcggaact gtggacaagc ctggaagacg gccagcgcct
    11101 gttgagccgg ggcgattcgt actcggcgat cgtggtgcag gccgcgccgg acgccgacgt
    11161 gcgcacactg caaaaccgaa tggcaggcgc gaccgtgctg gcgcgtgaga ccgacatcga
    11221 caccgagccg gaagtctatg aaaccatgaa tcgctcgttc gggcaggtga cgcgcgtgat
    11281 gaacgcgatt tccgcgctgg ccttgatcgc catcgccttc ggcgtgtaca acgtgg
//
LOCUS       DBNHXB010000236         7505 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_560438, whole genome shotgun sequence.
ACCESSION   DBNHXB010000236 DBNHXB010000000
VERSION     DBNHXB010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7505)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_560438"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="AC162G_09785"
     CDS             complement(<1..668)
                     /locus_tag="AC162G_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5076148.1"
                     /translation="MRNTIRQWILVLAAMLLAACGPSAPPAPLDAPSLTDQTIILWHS
                     ADGDERRALLDLVDQFNATNPWRILVVPEYHGSPADIAGALKVAIESGRTPDLLLGQP
                     IDVTWLGDSVVPVEPYASDPRFGLSDADMADFYPAFLDANRDPHRDGALVGFPVGGDG
                     TVLVYNADRLAAAGYLTPPSSWPLFREVCLVTTVDRDGDRRPDIYGFGFTPRPEFVSA
                     WFLSR"
     gene            1020..1880
                     /locus_tag="AC162G_09790"
     CDS             1020..1880
                     /locus_tag="AC162G_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR5076149.1"
                     /translation="MKSLTVGLAQIYPKLGDVRANCELHMQFVERAATQNVDVLIFPE
                     LSLTGYTLQDLVREVAMRPTAEHPAFAAMLDASRRLDLVVGFAEVDERGRFFTSAAYL
                     SQGKVVHLHRKVYLPTYALFDEGRYFDLGESVRAFNTRFGRFGILICEDFWHASPPYL
                     AWLDGADVLILTSSSPGRGLGYPGKLASSRFVETVNQAYAALFGSYVVHVNRCGFEDG
                     LNFWGGSTVFDPDGDLIAQAPYFEEALVVTQVHLDQIRRTRTRLPMLRDERPEVTLRE
                     LQRILSERSG"
     gene            1969..2799
                     /locus_tag="AC162G_09795"
     CDS             1969..2799
                     /locus_tag="AC162G_09795"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR5076150.1"
                     /translation="MNLDDKLRIDPDLVHTVLKHFLRDEIRKVGFEKAVIGVSGGVDS
                     AVACTLVAQAIGAHNVLALRLPYKTSSPDSLEHAQRVIDALGVPSETIDITPMVDPLF
                     ERLPDMDHVRKGNVMARQRMIVWYDRSAAIRGLVVGTSNKTELLLGYGTLFGDMAAAV
                     NPIGDLYKTQVRQLAAYLGVPQPIIDKPPSADLWVGQTDEGEMGLTYAEVDRLLTLLV
                     DERYTPAEAVEAGFEAKFVERVWRTVRVNHFKRRGPIIAKLSSRTIGVDFNYLRDWGY
                     "
     gene            2804..5158
                     /locus_tag="AC162G_09800"
     CDS             2804..5158
                     /locus_tag="AC162G_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR5076151.1"
                     /translation="MTQEMPLVSNLNLIPKALQLYLEISQYPILAKRIREQMRAELFS
                     RGVITPEAFEQEVQAKAIRSQELEGLSNPYEQEPADVWQARVEKVRDDVTDFYFAFNL
                     PHDLFRDIVQRLIGQRTPAHEVILAFNPELAPWDLLFAQGRAYESTPPEKRAQIEHHL
                     REIIVVLIKGMISDQLDLVRVAREVFTIQDLREIYDRRIGRGKIGGKAAGMMLAYKII
                     QREGQRHGIDVTRFITIPDSYFIASDAFYDFHASNNLFPYMNQKYKSHDRMREDYPAV
                     FRAYVDSQLPEYVEQALARLLERLSGAPLIVRSSSLLEDNFGKSFAGKYDSFFLPNQG
                     TLEENLAALADAIKKVYASVIRPEALIYRETVGLTDYDERMAILIQKVEGAPHHGYFL
                     PAFAGVAFSKNPYRWSPRVRLEDGMLRMVTGLGTRAVERVDNDYPRMVALSHPTLRPE
                     QSASAVRRYSQHQVDVLDLEANAFTTLPVADVLGADFPALGAIMSHDRGDYVQPFSGR
                     PLSTERQNMIVTFDRLLAHKPFVETMRSMLRVVEEAYGRPVDMEFAGEVVSTYPELQF
                     KVALLQCRPLSLRQPDQRYTIPEDVPPEAILFTANRQVPHGSASGIRYIVYVDPRRYA
                     RIPDANMRFEIGRVVGRLNQALAGAPFVLMGPGRWGTSNARLGVKVTYADIYNTKVLI
                     EIAHEDEGSVPEVSYGTHFFLDLVEADIYPLPLYPDDPSTVYKEEFFAGAANALPQLL
                     PADEAYAPYLKVIDVPAASGGRTLTIVMNAEQDKAIGYLTDGAA"
     gene            5145..6140
                     /locus_tag="AC162G_09805"
     CDS             5145..6140
                     /locus_tag="AC162G_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HIR5076152.1"
                     /translation="MAPHNWSAGFSDVGRLSIAAFKAALQHSQGQVGMKRAVSISLGS
                     AKRDKVVEVDLLGERVRVERIGTDGDISWAAALFAELAGKVDAFGVGGIDLGEHTPWK
                     FYPLRDAQRMVKHVQRTPIVDGGGLKMTLEARVMQAVEREIGANIRPKTALLVAAITR
                     YGMTASFVNAGYECVFGDLMFALGLPVPVRSLTTLRRLAKVLLPIAGRLKIEWLYPTG
                     DSQEKNAPKYEKHYQWASVTAGDFLYVRKHMPPRMDGKTIVTNTTTGADVDFLRARGV
                     KYLVTTTPVYDGRSFGTNLLEAALVAATGKGRALTHDELDALIDQLKLGPQIQRL"
     gene            6200..6970
                     /locus_tag="AC162G_09810"
     CDS             6200..6970
                     /locus_tag="AC162G_09810"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5076153.1"
                     /translation="MDAIVVAGQAPGHTDALLAYAGAGNKALIPIAGKPMIKWVTDAL
                     AGSRYVDRLIVVGLDESCGIDFGPKPVAYVPSQERLLQSALAGVRRALQDTSDRDHLL
                     MCACDIPLITTTIVDDHIVTMLATQHDLYYTVIDRETMLTRFPTSRRSYARLRDGVFC
                     GGDLNMGSSKVMETDRQLWDDLLEARKSALRQARRVGFGILLRFATGRLSLAAAACRA
                     EQILGVKCRVVRSPHAELGMDVDKPFQLEIVRAELEKR"
     gene            complement(6979..>7505)
                     /locus_tag="AC162G_09815"
     CDS             complement(6979..>7505)
                     /locus_tag="AC162G_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076154.1"
                     /translation="LLFLLGALTDLSKWGLLFPGFFLLGIGVVIFLSESASASGNVIG
                     SVFLASVALPFWAVALVRRANWWALIPAGVITTLAMMPLLAETQMRGEVVAGIFFMGL
                     GATFGLVRLATIGQSGTSWAWYPAVILGGFGLALMVSNNPQAWPLVLIGAGVLLLVRS
                     FLPRRRTDQPDAKG"
BASE COUNT         1422 a         2421 c         2413 g         1249 t
ORIGIN      
        1 cggctcagga accaggccga cacgaattcc gggcgcggcg tgaagccaaa cccgtagatg
       61 tccggccggc ggtcgccgtc ccggtcgacc gtggtgacca ggcatacttc gcgaaacagg
      121 ggccacgagc tcggcggcgt gaggtagccg gccgcagcca ggcggtcggc gttgtagacg
      181 agcaccgttc cgtcgccgcc gaccgggaaa cccaccagcg cgccgtcccg atgcgggtca
      241 cggttagcgt cgaggaaagc cgggtagaaa tcggccatgt cggcgtcgct caagccgaag
      301 cggggatcgc ttgcgtacgg ctcgacgggc acgaccgaat cgcccagcca cgtcacgtcg
      361 atcggctggc cgagaagcaa atcgggagtg cgacccgatt cgatcgcgac cttgagcgca
      421 cccgctatgt ccgccgggct gccgtgatat tcgggcacga ccaggatgcg ccaggggttc
      481 gtcgcattga attggtcgac cagatcgagc agcgcgcggc gctcgtcgcc gtcggcgctg
      541 tgccacagga tgatcgtctg gtctgtgagg ctgggtgcat cgagcggtgc gggcggcgcg
      601 gacgggccgc acgctgcgag cagcatcgcg gccaggacga ggatccattg tcgaatcgta
      661 tttctcatgg cgacggcgcg gattataaca cgcgcggagc gcctgtccct cacgtgagac
      721 ccattcgagc cacggccaag acctcggaag tctcagattg aagacttccg aagtctgaca
      781 ctcgcccttg acagcctctc gatttgggct atatttagtg tcgaaagtac accaattaag
      841 ccatcaagaa acgtggctgc tcaagcgtca ttgcgaggag cgtcgtttgc gacgaagcaa
      901 tctcagccgc cctgagcgcg cgtggctgtc cgtgccggag attgcttcgc aaaaaagcgc
      961 tcgcaatgac gtgggttgag caattaccga gaaaccaggt ttctgaaata ggtgatgcga
     1021 tgaaatccct gaccgtcggt ctggcgcaga tctatcccaa gctgggtgac gtgcgcgcca
     1081 attgcgagct gcacatgcag ttcgtcgaac gtgccgccac gcagaacgtc gacgtgctca
     1141 tctttcccga gctgtcgctg accggctaca cgctgcagga cctggtgcgc gaggtggcga
     1201 tgcgccccac ggcggagcac ccggcgtttg ccgccatgct cgacgccagc cgccgcctcg
     1261 acctggtggt cggcttcgcc gaagtcgacg agcgcgggcg gttcttcacc tcggccgcct
     1321 acctgtcgca gggaaaggtg gtgcacctgc accgcaaggt ctacctgccg acgtatgcgt
     1381 tgttcgacga agggcgttac ttcgacctgg gcgagtcggt gcgcgcattc aatacgcgct
     1441 tcggaaggtt cggcatcttg atctgcgagg atttctggca cgccagcccg ccgtacctgg
     1501 cctggctcga cggcgcggac gtgctgatcc tcacgtcgtc cagcccgggg cgcggcctgg
     1561 ggtatccggg caagctcgca tcctcgcggt tcgtggagac ggtgaaccag gcctacgctg
     1621 cgctgttcgg ctcgtacgtc gttcacgtca atcgctgcgg cttcgaagac gggctgaatt
     1681 tctggggcgg ctcgaccgtg ttcgaccccg acggcgatct gatcgcacag gcgccgtatt
     1741 tcgaggaggc cctggtcgtc acgcaagtcc atctcgacca gatccgccgc acgcgcacgc
     1801 gcctgccgat gctgcgcgac gagcgtcccg aagtaaccct gcgcgagctt cagcgcatcc
     1861 tgtccgaacg atccggctag tcctgagttc cttcagttcc cttgagttcc ttgagttcca
     1921 agggagctga aggaactcac gggaactcct taaagaatga gagccatgat gaatctcgac
     1981 gacaagctcc gcatcgaccc cgatctggtg cacaccgtgc tcaagcattt tctgcgcgac
     2041 gagatccgca aagtgggttt cgagaaggcg gtgatcggcg tgtccggcgg cgtcgacagc
     2101 gccgtggcgt gcactctcgt cgcgcaggcg atcggcgcgc acaacgtgct ggcgctgcgc
     2161 ctgccctaca agacgtcgtc gccggacagc ctggagcacg cgcagagggt catcgacgcg
     2221 ctcggcgtgc cgagcgagac gatcgacatc acgccgatgg tcgacccgct gttcgagcgc
     2281 ctcccggaca tggaccacgt gcgcaaaggc aacgtgatgg cccgccagcg catgatcgta
     2341 tggtatgatc gatcggcggc gatcaggggc cttgtcgtcg gcacgagcaa caaaaccgag
     2401 ctgctgttgg gctatggcac gctgttcggg gacatggcgg cggcggtgaa cccgatcggg
     2461 gatctttaca agacgcaggt gcgccagttg gccgcgtacc tcggcgtgcc gcagccgatc
     2521 atcgacaagc cgccctccgc cgatctgtgg gtcgggcaga cggacgaggg cgagatgggg
     2581 ctgacgtacg cggaagtcga ccggctgctg acgctgctcg tcgacgagcg ctacacaccc
     2641 gccgaagcgg tcgaggccgg cttcgaggcg aagttcgtcg agcgtgtgtg gcgcacggtg
     2701 cgcgtcaatc acttcaagcg gcgcggtccg atcatcgcca agctgagcag ccggacgatc
     2761 ggggtcgatt tcaattacct gagggactgg ggatattgac tgcatgaccc aagaaatgcc
     2821 gctcgtctcc aacctgaacc tcattccgaa ggcgctccag ttgtacctgg agatcagcca
     2881 gtatccgatc ctcgccaagc gcatccgcga acaaatgcgc gccgagctgt tctcgcgcgg
     2941 cgtcatcacg ccggaggcgt tcgagcagga ggtgcaggcc aaggcgatcc gctcccagga
     3001 gctggagggc ctgagcaacc cgtacgaaca agagccagcc gacgtgtggc aggcgcgcgt
     3061 ggagaaggtc cgcgacgacg tcaccgattt ctatttcgcc ttcaacctgc cgcacgatct
     3121 gttccgcgac atcgtgcagc gcctcattgg tcagcgcacg ccggcgcacg aggtcatcct
     3181 cgcgttcaat cccgagctgg cgccgtggga cctgctcttc gcgcagggca gggcctacga
     3241 gagcacgccg ccggaaaagc gcgcccagat cgagcaccat ctgcgcgaga tcatcgtcgt
     3301 gctcattaaa ggcatgatct ccgaccagct cgacttggtg cgcgtggcgc gcgaggtctt
     3361 cacgattcag gacctgaggg agatttacga ccggcgcatc gggcgcggca agatcggcgg
     3421 caaggctgcc ggcatgatgc tcgcctacaa gatcatccag cgcgaaggtc agagacacgg
     3481 catcgacgtg acgcgcttca tcaccatccc cgactcgtac ttcatcgcct ccgacgcctt
     3541 ctacgacttt cacgccagca acaatctgtt cccctacatg aaccagaagt ataagagcca
     3601 cgaccggatg agggaggatt atccggctgt cttccgggcc tacgtcgaca gccagttacc
     3661 ggagtacgtc gagcaggcgt tggcgcgcct gctcgagcgg ctgagcggcg cgccgctcat
     3721 cgtgcgctcg tccagcctgt tggaggacaa cttcggcaag tcgttcgccg gcaagtacga
     3781 cagttttttc ctgcccaacc agggcacgct cgaagaaaac ctggcggcgc tggccgatgc
     3841 gatcaaaaaa gtgtacgcca gcgtgatacg tcccgaggcg ctcatctatc gcgagacggt
     3901 cggcctgacc gactacgacg agcgcatggc gatcctgatc cagaaggtgg agggagcgcc
     3961 gcatcacggc tatttccttc cggccttcgc cggcgtggcc ttcagcaaaa acccctaccg
     4021 ctggagcccc cgcgtccgcc tcgaagacgg gatgctgcgc atggtgaccg ggctgggcac
     4081 gcgcgccgtc gagcgggtcg acaacgacta tccgcgtatg gtcgcgctca gccatcctac
     4141 actgcggccc gagcagtcgg ccagcgccgt gcgccgctac tcgcagcacc aggtcgacgt
     4201 cctcgacctg gaggcgaacg ctttcacgac gctgccggta gccgacgtgc tgggtgcgga
     4261 ttttccggcg ctcggcgcga tcatgtcgca cgatcgcggc gactacgtcc agcccttctc
     4321 cggccggccg ctgtcgacag agcggcaaaa catgatcgtc acgttcgatc gcctgctggc
     4381 gcacaagccg ttcgtcgaga cgatgcgcag tatgctgcgc gtcgtcgagg aggcttacgg
     4441 gcggccggtc gacatggaat ttgccggcga agtggtctcg acttatcccg aactgcagtt
     4501 caaggttgcg ctgttacagt gccggccgtt gagcctgcgc cagcccgacc aacgctatac
     4561 gatcccggag gacgtcccgc ctgaagcgat cttgttcacg gccaaccggc aggtgcctca
     4621 cggctctgcc agcggcatcc gttacatcgt ctacgtcgat ccgcgccggt acgcgcgcat
     4681 cccggatgcc aacatgcgct tcgagatcgg tcgcgtcgtc gggcgcttga accaggcgct
     4741 ggccggcgcg ccattcgtcc tcatgggacc gggacgctgg ggcacttcga acgcgaggct
     4801 gggcgtcaag gtaacgtacg ccgacatcta caacacaaag gtgctgatcg agatcgcgca
     4861 cgaggacgaa ggcagcgtgc cggaggtctc gtacggcact cacttcttcc tggacctcgt
     4921 cgaggccgac atctaccccc tgcccctgta ccccgacgat ccgtcgaccg tctacaagga
     4981 agaattcttc gcgggcgccg cgaacgcgct gccccagttg ctgccggccg acgaggcgta
     5041 cgcgccttac ctcaaagtca tcgacgtgcc ggcggcgagc ggcggccgca ccctgacgat
     5101 tgtgatgaac gccgagcaag acaaagccat cggctacctg accgatggcg ccgcataact
     5161 ggagtgcagg cttcagcgac gtagggcggc tttcgatagc cgccttcaag gctgcactcc
     5221 agcacagcca ggggcaagtc ggcatgaagc gtgccgtcag catcagcctg ggcagcgcga
     5281 aacgagacaa agtcgtcgag gtcgatctgc tcggcgagcg ggtgcgcgtc gagcgcatcg
     5341 ggactgacgg cgacatctcc tgggcggcgg cgctcttcgc cgaactggcc ggcaaggtcg
     5401 acgcgttcgg cgtcggcggc atcgacctgg gcgagcacac cccgtggaag ttctacccgc
     5461 tgcgcgacgc gcagcgcatg gtgaagcacg tccagcgcac gccgatcgtc gacggcggcg
     5521 ggctgaagat gacgctcgaa gcgcgcgtga tgcaggcggt cgagcgcgaa atcggcgcga
     5581 acatccggcc gaagacggcg ctcctcgtcg ccgcgatcac ccgctacggc atgaccgcgt
     5641 cgttcgtgaa cgccggatac gagtgcgtgt tcggcgactt gatgttcgcg ctcggcctgc
     5701 cggttccggt gcgcagcctg accacgctgc gccgcctggc gaaagtgctg ctgccgatcg
     5761 ccgggcgctt gaagatcgag tggctgtacc ccaccggcga cagccaggag aaaaacgcgc
     5821 cgaaatacga gaaacactac caatgggcca gcgtgaccgc cggcgacttc ctgtacgtca
     5881 ggaaacacat gccgccgcgc atggacggca agacgatcgt gacgaacacg acgaccggcg
     5941 ccgacgtgga tttcctgcgc gcgcgcggcg tgaaatacct ggtgacgacc acgccggtat
     6001 acgatgggcg ctcgttcggg acgaacttgc tggaagcggc gctggtcgcc gccacaggca
     6061 aaggccgcgc gctgacgcac gacgagctgg acgcgctgat cgatcaactc aagctcggcc
     6121 cgcagattca gcgcctgtaa gatccctgag ttccttgagt tccctcaaat caggaactca
     6181 aggagctgaa ggaacttccg tggatgcaat tgtcgtcgcc ggacaggcac ccggccacac
     6241 cgatgcgctg ctggcgtatg ccggcgccgg gaacaaggcg ctgatcccga ttgccggcaa
     6301 gccgatgatc aagtgggtaa ccgacgcgct ggccggctcg cgctacgtgg atcgcctgat
     6361 cgtcgtcggg ttggacgaat cgtgcgggat cgacttcggc cccaagccgg tggcgtacgt
     6421 gccgagccag gagcggctgc tccagagcgc gctggccggc gtgcgccgcg ctctgcaaga
     6481 cacatccgac cgcgatcatc tcctgatgtg cgcctgcgac atccccctga tcacgacgac
     6541 catcgtcgac gatcacatcg tgacgatgct ggccacgcag cacgatctgt attacaccgt
     6601 catcgatcgg gagacaatgc tcacccgctt cccgacctcg cgccgatcgt acgcgcgcct
     6661 gcgcgacggc gttttttgcg gcggcgattt gaatatgggc agcagcaagg tcatggagac
     6721 cgaccggcaa ttgtgggacg acttgctcga agcgcgcaag agcgcgctgc gccaggcgcg
     6781 gcgcgtcggc ttcgggatac tgctgcgctt cgccacgggc aggctgtcac tcgccgccgc
     6841 cgcgtgccgc gccgagcaaa tcctcggcgt gaagtgccgc gtcgtgcgct cacctcacgc
     6901 tgagctcggg atggacgtag acaagccgtt tcagttggag atcgtgcgcg cggagttgga
     6961 gaagaggtaa gaaggacgct accctttcgc gtccggctga tccgttcggc ggcgcggcag
     7021 aaacgaccgg acgagcaaca agacgccagc gccgatcaac accagcggcc acgcctgcgg
     7081 attgttcgac accatcagcg ccaggccaaa gccgccgagg atcaccgccg gataccacgc
     7141 ccagctcgtg ccgctctgtc cgatcgtcgc caggcgcacc aatccgaacg tggcgcctaa
     7201 tcccataaag aatatcccgg ccacgacttc accgcgcatc tgggtctcag ccaggagggg
     7261 catcatcgcc agtgtggtga tcaccccagc cggaatgagc gcccaccagt tcgcccgccg
     7321 gacgagcgcc acggcccaga acggcaaggc gacgctggcg aggaaaacag agccgatcac
     7381 gttaccggat gccgaggcgc tctcagaaag aaaaatcacg acgccgatac ccagcagaaa
     7441 gaagccgggg aagagcagtc cccacttcga gagatcggtc agtgcgccga gcaggaagag
     7501 caggc
//
LOCUS       DBNHXB010000237        10534 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4083844, whole genome shotgun sequence.
ACCESSION   DBNHXB010000237 DBNHXB010000000
VERSION     DBNHXB010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10534)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10534
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4083844"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="AC162G_09820"
     CDS             <1..196
                     /locus_tag="AC162G_09820"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR5076155.1"
                     /translation="SADDYRRIAPEFGADLAETLDFVHSIAARETIGGTAPRAVREQI
                     AQARKLLSDSALLPLIASIA"
     gene            complement(177..878)
                     /locus_tag="AC162G_09825"
     CDS             complement(177..878)
                     /locus_tag="AC162G_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076156.1"
                     /translation="MTAISTVSTALVVLIILLLLGSLFTGSVLADAEIFKPYTGHRMN
                     VETDAMAQRLKVQAEADARVAVVQAQEAAKLAAAKTAAEQDLIRADTQAQIAVIQAET
                     EATIARIQEDLRVQIRQNDQKIENDANINKVVTILALAFGLGLCVAFARLLFGLAGLA
                     STRPSTVPAITATQTRAVDSWRDSDFRAQAIGQARKREREYRESLIARRTVWPEDIRS
                     GNGRKGPVSVRQSKR"
     gene            complement(1261..1755)
                     /locus_tag="AC162G_09830"
     CDS             complement(1261..1755)
                     /locus_tag="AC162G_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HIR5076157.1"
                     /translation="MRITGWMARHGIPFLRMSLGIVFFWFGVLKFFPGLSPAQDLATR
                     TIDILTFGLIPAHLSMLVLATWECLIGLGLIFGVFLRATLLLLFLQMLGTILPIFFFP
                     QEVFTRIPYAPTLEGQYIIKNVVLIGAGIVIGATVRGGAVVADPRIARVAVQMEKRQE
                     KEMQ"
     gene            2207..3484
                     /locus_tag="AC162G_09835"
     CDS             2207..3484
                     /locus_tag="AC162G_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5076158.1"
                     /translation="MNRIPSIALLLALLLMAGCAAPATTPATTAAPPIVIGVPTDLGT
                     IEGQDALNSALLAVDEINAKGGVNVGGAQRLLQIESIDTREAGAGIPVNDALQAVEKL
                     ILEKEPDAIVVGAFRSEVLMASLDLIAKYKIPYLVTIAATPALQQRVTESYDSYKYFF
                     RLGLNGSGAGMYLTQAMGFLAQEFNFSRAYLTYQDVAWAKGTADGVAKWLTDNGWEVV
                     GNDAYPIGATEFSTSLSKAIDGKADVIMPIFDMPQAGILLKQARSMQAPALLAGFISP
                     VAPATAWETFEGDVDGMVNLMFEIGPLPVQAVPASVQFNDNYAKKFGEEARAKLSGHG
                     PGPSYDAVYVLAAAIERAGTLDGDALVTALEATDMQGVIGRIKFGEDHQVIFGLDPKE
                     TALGVVFQWRDGKRIPVFPPAVAEGEIEVPASE"
     gene            3584..4456
                     /locus_tag="AC162G_09840"
     CDS             3584..4456
                     /locus_tag="AC162G_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5076159.1"
                     /translation="MIASVLIYGLVNSVILILTALGFSLTFGLSKVANFAHGGLYLFA
                     GLAAWLALHNLGLPYGIAILLAILVTGALGALIYRFILLRVRGIELSEVIATFAVGVA
                     ILEFFRSLGFVTYEFNLAPFIKGQLRIAGVSIDYQRLFVIGVGLALAVLLWLFTHHTK
                     IGLALRGIAQDEHTALAFGIESDWAAMLSVALGSALTAIAALTIFPLSIISIGTGYEV
                     LLIAVAVSVLGGLESTPGLIIGGLGLGYSQVLAATILGTEWQAVVYLAAIVLVLAVKP
                     SGLLGKSQELEERV"
     gene            4458..5483
                     /locus_tag="AC162G_09845"
     CDS             4458..5483
                     /locus_tag="AC162G_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5076160.1"
                     /translation="MSAAYRRERLDRGIKARSDDIFALASYRELSYLLGPRVVLIGGL
                     LIFPLLRDVIGVYGLNVMLTACTIALMALSWDLLASAGLVSLGQALFFGIGGYLAGVL
                     NDTYHWPPFLTIPAGTLLGALLCAAILYPVLRLRGIYFALITLTLPLLFMRVIEATKI
                     LGGTDGLSALDPLPDITIVLYIAIAAVLLTLFGFRRLINTDYGLVLKGIRDNDRSVIA
                     AGINITWYKAQAVFIAALPATFAGALMTHQLQFVGMPAFASDYSILPLTGAIVGGVGT
                     FAGAMLGAFLLVPISEIFRGFGTWRVVVYSVILVVFVVGLPEGLFHYVQRRYHQFERR
                     VPWEGDT"
     gene            5480..6244
                     /locus_tag="AC162G_09850"
     CDS             5480..6244
                     /locus_tag="AC162G_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076161.1"
                     /translation="MSAVPILKVEQLNKRFGGVGAVYNVSFELEEGGALGVIGPNGSG
                     KTTLVNLITGFVRPTSGRVLLKGKDITRLMPHERVGLGIARSFQMVRPFYNLPAFKNL
                     IIPLYSPRVRRMKGGQYGNRDEVAIHLLEEVGFERDSQVPYKTASNLPHGYLKRLELA
                     RCLALEPEVLILDELFSGMSLSEVASLLPLIEKIRERGTTLMMVEHRLRELFRIVKTV
                     IVLNFGEKIAEGTPSEVIEMEDVRKAYLGTGVGADA"
     gene            6237..6980
                     /locus_tag="AC162G_09855"
     CDS             6237..6980
                     /locus_tag="AC162G_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076162.1"
                     /translation="MLEIKDLTVYYENALALNSLSLEVRPGEIVGVFGSNSAGKTTRM
                     NTIAGLTLDLKTKEERKGGERITVHGELKFEGEEILHAKPSDRVKKGIVLCRERHPVF
                     RDSSVEENLKIGGFLRPSREVKERIRLVYDIFPHLTRLRRRKGGLLSGGEQQMLAVGM
                     ALVAHPRLLLLDEPLLGLSPLLQADIVRAMRDIRQQGITLLVSEQYARPIFPVIDRGY
                     VIENGTLILTGTGPELMNNPEVRSAYFGV"
     gene            6983..8632
                     /locus_tag="AC162G_09860"
     CDS             6983..8632
                     /locus_tag="AC162G_09860"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR5076163.1"
                     /translation="MDSIYHVFENTANSLPEKSCLIYLGKKYTFAQLADAVDRAATSL
                     TQWGVKKGSQAILFLPNLPQWIIAWLALQKMGAVAIPITPVYAPSDLEYIANDSGAET
                     IFCLDTNFGYVTRVLSETKLKRVVVTTLIEALPLWKRVLGRALNKVPKGMFHLDENTI
                     AFSQLLKTRGRSGREPAPNGVKGLASSPGLEIAEMLYTGGTTGYPKGVPISHALFLEA
                     MREQRRAGEGLVPKGQDVLIQGAPLYHILGQVVGLGALLHGEQMILLPRPSLDATFDH
                     IQRYKATTFFGVPTMYRMILEHDRVDQYDLGSLKYCFSGGDVLPVEVAHRWRKKFGKP
                     IHQGYGATEAGGGISLTPAGEPFPEGSVGKVVPFQKIKIVNPDTLEPLPVGESGELLV
                     SSEHMVNAYWNKPEETLRSFVNLDDKRWYRTGDIVRLDDQGWLFFRDRSVDVIKHKGY
                     RVAASRIEAVLQEHPAVIASCVVGVPDSNVGERIKAFVVLKEDVKGITAYDLTTWCRG
                     RLAPYEVPHYIEFRDMLPKSKVGKLLRRELRAEEQRKMAAA"
     gene            8739..>10534
                     /locus_tag="AC162G_09865"
     CDS             8739..>10534
                     /locus_tag="AC162G_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR5076164.1"
                     /translation="MKRALTLLLALMFLVGSAEGLPSRAAATAAWLPVNGPTGGSVSA
                     LAISPDYAYDHTAYAGLRGRGVYRTDDGGLSWKSTSPDDWVVVDLAISPAYAADQALF
                     AVTGFGTTGYTVYRSTDAGETWQAPATVPTGGLQNLTGVVVSPNYAVDRTLYAIGASQ
                     TYRSTDGGIIFTEAGGWFSTHPLTDLAFSPAYAADRTLYATASGGDPAGLYKSTDGGT
                     VWTPTGLTGNLSAVAVSPDYADDQTIAAINTDDGRITISPDRGASWSSSTITLSGSAD
                     QHTLLFSPTFAADGIMLAASAADPGAYRSTNGGATWSPVGWYDSTKPYLGGFVGGSVF
                     ALALAPTTASDAAAFAGTSSGIYRSSNWGEHWYPGNKGLARLTIRALAVAPNDPHTLL
                     AGTAFFERALTGNPDEDAGSLQLSTDGGATWRDVSGRLDQVQQVAFSPDFAADRTAFA
                     VTGLTAPPDFRSGGIYRSTDGGQNWSLVSSGLIGWAVAISPDFAADGTVWVYGSSATE
                     SGIFVSADGGDSWALLASSPLLAPRPAGSAGVIAPSPNYAVDRTIFAGTGDGGVQRSP
                     DGGQTWTQVLSHTTTALAVSPLYAASQTVYAGV"
BASE COUNT         2027 a         3086 c         3280 g         2141 t
ORIGIN      
        1 gtcggcggac gactatcgcc ggatcgcccc agagtttggg gcagacctcg cagagacact
       61 cgattttgtc cattcgatcg cggcgcgcga gacgatcggc ggcacggctc cccgggcagt
      121 gcgcgagcaa atcgcccagg cgcgtaaact cctcagcgac tccgcactcc ttccgctcat
      181 cgcttcgatt gcctgacaga tacaggcccc ttgcgaccgt taccgctgcg tatgtcctct
      241 ggccacacgg tgcggcgggc gataagcgac tctcgatact cgcgctctcg tttcctagcc
      301 tggccgatgg cctgggctct aaaatccgaa tcgcgccacg aatcgacagc ccgagtctgc
      361 gtagctgtga ttgcaggcac agtggacgga cgcgttgagg cgagccccgc caaaccaaac
      421 agcagcctgg cgaaggcaac gcacaggccc agcccgaagg caagtgcgag gatcgttaca
      481 accttgttga tattagcatc attctcaatc ttctgatcgt tctgcctgat ctgcacgcgc
      541 aggtcttcct gaattcgcgc gatcgtggcc tcggtttcag cctggataac cgcgatctgg
      601 gcctgggtgt cggcccggat gagatcctgc tcggctgccg tcttggcagc ggcaagtttt
      661 gccgcctctt gggcctggac taccgcgact ctcgcatcgg cttctgcttg caccttgagc
      721 cgttgtgcca tcgcgtcagt ttccacattc attcggtggc cggtataggg cttgaagatc
      781 tcggcatcgg ccagcacaga cccagtgaac agtgagccga gtagcagtaa gataatcaac
      841 acgaccaacg ctgttgaaac ggtactgatc gcagtcatgt cacacctccg ttcgcagaaa
      901 caggtcccgc tcctccattg cgtgtcgttt tcatgcggca gtgtgattcc agatggacac
      961 cattaccgca gacataaagg ccgtcagtag aaatttcctg cacggccaac gtgcacgacg
     1021 tcgtggtctg gattggacag agcgataacc gtcaacgcgg agagattgcg ctcccgatgg
     1081 ctgataacag gatagtccga tacgaaggtg caatcaatgg gggattcccc ttgatggcga
     1141 ggggagaata ccgggcagcg gtagggacat ccctaccctg atgcgagact tatgtcagca
     1201 cacggcggcg gaacgtacct attcatcatg ccacgcggca attgattaag cgagattaag
     1261 tcattgcatc tctttttctt gccgcttctc catctgcaca gctacccggg caatcctggg
     1321 atcggcgacg actgcgccgc cacgcacagt cgcgccgatc acgatgccgg cgccgatcag
     1381 caccacgttc ttgatgatgt attgaccctc cagggtgggc gcgtagggaa tgcgagtgaa
     1441 cacttcctgg gggaagaaga aaatgggcag gatcgtgccg agcatttgca gaaacaacag
     1501 gagcaacgtc gcccgcagga acacaccgaa tatcagcccc aacccgatca ggcattccca
     1561 tgtggccagc acaagcatag agagatgggc agggataagc ccgaaggtga ggatatcgat
     1621 cgtgcgtgtc gccaggtcct gcgccgggct aagaccgggg aagaacttca gcacgccgaa
     1681 ccagaagaag acgatgccga ggctcattcg caagaatggg atgccgtgac gagccatcca
     1741 tccggtaata cgcacatcta tatcatcgaa ccatcgaggt agactttgca tgattcacct
     1801 gccgggtaga aggggtcgga tgcgccacat aacaggatat tcggatgcga aggagcaatc
     1861 aatgggggat tccccttgat ggcggaggga gattaccggg tagcggtagg gatatcccta
     1921 ccctgatgcg acgcttatgc cagcacacgt cattttccaa tggacttggg accaataccc
     1981 atgtcgagta caagaacact gcacactggt cgaatgacga gaacaaagag aagtttgaca
     2041 gcttaaatta tatcgtatta taataaaaca taccgggtag aaggtattta attgcatgct
     2101 caaagtccgt aacgatcagc gccaacctgg tggcggtggt acggactttt cctcatagct
     2161 gtcagccggg acaccacgtt gctatccttt caaggaggac atcaaaatga accgcattcc
     2221 tagcatcgcg ttactgctcg ctctgctgtt gatggcgggg tgtgctgcgc cggccaccac
     2281 gccggcgacg acggcggccc cgccgattgt gatcggtgtg ccgacggacc tgggcaccat
     2341 cgaggggcaa gatgcgctca actcggctct gctggcggtg gatgagatca acgccaaagg
     2401 cggggtgaat gtgggaggtg cgcaacggct cctgcaaatc gaatcgattg acacacggga
     2461 ggctggcgcg ggcattccgg tgaacgatgc cctgcaagcc gtggagaaac tgatcctcga
     2521 aaaggagcca gacgcgattg tggtcggcgc tttccgttcc gaagtcttga tggcctcgct
     2581 ggacctgatt gccaagtaca agatcccgta tctcgtcacc attgccgcga cgcccgcgct
     2641 ccagcaaaga gtcacggaga gctatgacag ttacaagtac ttcttccgct taggcctgaa
     2701 tgggtcgggc gccggcatgt acttgacgca ggcgatgggc ttcttggcgc aggaattcaa
     2761 cttcagccgc gcttacctca cttaccagga cgtagcgtgg gcgaagggaa ccgccgacgg
     2821 tgtggcgaag tggctcacgg ataacggctg ggaggtcgtc ggcaacgacg cgtacccgat
     2881 tggcgcgacg gagttctcta catcgctgag caaggcgatc gacggcaaag ccgatgtgat
     2941 tatgccgatc tttgacatgc cacaggcagg tattctattg aagcaggcgc gctcgatgca
     3001 ggcgccggcc ctcctggcgg gctttatcag ccccgtggct ccggcgacgg cctgggaaac
     3061 attcgagggg gatgtggacg ggatggtcaa cttgatgttc gagattggcc ctctgccggt
     3121 tcaagccgtt cccgcttcgg tgcagttcaa cgacaactat gccaagaagt tcggcgaaga
     3181 ggcgcgggcg aaactcagcg ggcacggtcc tggaccttcc tatgacgcgg tctacgtcct
     3241 cgccgcagcg attgagcgcg cgggaacgct cgacggcgat gcgctcgtca cggcgttgga
     3301 agcgaccgac atgcaagggg tcatcgggcg gatcaagttt ggcgaggatc accaggtgat
     3361 cttcggtctc gatcccaagg agaccgcgct cggcgtagta ttccaatgga gagacggaaa
     3421 acgcatcccg gtcttcccac cggccgtcgc cgaaggcgag attgaagtgc cggcatccga
     3481 ataagcctcg tgtgtactgg cgcctgagca tctaacgaat gagtcaaccc cgggagggcg
     3541 acctgcatcg aggccgccct ctccttgtcc acaggtcgga cccatgatcg ccagcgttct
     3601 catctatggc ctggtcaaca gtgtcatcct gatcctcacg gcgctcggct ttagtctcac
     3661 gttcggtttg agcaaagttg ccaattttgc gcacggcggg ttgtatctgt tcgcgggtct
     3721 tgccgcgtgg ctggcgctgc acaatctcgg gttgccttac ggcatcgcta tcctgttggc
     3781 cattctcgtg accggcgccc tcggcgcctt gatctaccgc ttcattttgc tgcgcgtgcg
     3841 tggcatcgag ttgtccgaag tgatcgcgac attcgccgtg ggcgtcgcga ttcttgagtt
     3901 cttccggtcg ctgggattcg tcacctacga gttcaacctg gctccattta tcaaggggca
     3961 gcttcggatc gcaggcgtgt cgattgatta ccagcggctg tttgtgatcg gcgtcggatt
     4021 ggcgctggcg gttttactct ggctgttcac gcaccatacg aagatcggcc tggcgctgcg
     4081 cgggatcgca caggacgagc acaccgcgct ggcgttcggc atcgaatcgg actgggcggc
     4141 gatgctcagc gtggctctcg gctcggcgct gacggcgatc gcggcgctga cgatattccc
     4201 gttgagcatc atctcgatcg gcacggggta cgaagtgcta ttgatcgcgg tcgccgtgtc
     4261 cgttttgggt ggcctggaaa gcacacccgg cctgattata ggcggactcg ggcttggcta
     4321 ttcgcaagtg ctggccgcga cgattttggg aacggagtgg caggcggtgg tgtacctcgc
     4381 ggcgattgtc ctcgtcctcg ccgtcaagcc gtcggggttg ctcggcaagt cccaggaact
     4441 ggaggaacgc gtttgacatg agcgcggctt accgcaggga gcggctggat cgaggcatca
     4501 aagcgcggtc ggacgatatc ttcgcgctcg cgtcttatcg cgaattgtcc tacctgctgg
     4561 gaccgcgcgt ggtgttgatc ggcggtctgc tcatctttcc tttgctgaga gatgtgatcg
     4621 gagtgtatgg gttaaacgtg atgctgaccg cctgtacgat cgcgctcatg gcgctctcct
     4681 gggacttgct cgcgtccgcc gggctggttt cgctggggca ggcgctgttc ttcggcattg
     4741 gcggctatct cgccggtgtc ctgaatgata cttaccactg gccgcctttc ctcaccattc
     4801 cggccggcac gcttctcggc gcgctgttgt gcgcggccat cctctaccca gttctgaggc
     4861 tgcgcgggat ctactttgcg ctcatcaccc tgacgctgcc cctgctattc atgcgcgtga
     4921 tcgaagcgac caagatcctg ggcggcacgg atgggctgag cgcactcgat cccttgccgg
     4981 acattacgat cgtgctgtat atcgcaatcg cggcggtgct tctcaccctg tttggatttc
     5041 gccgtctcat caacaccgat tacggcctgg tgttgaaggg gatcagggat aacgaccgct
     5101 cggtgatcgc ggcggggatc aacatcacct ggtacaaggc ccaggccgtg ttcatcgccg
     5161 cactccccgc gacgttcgcg ggggcgctga tgacgcatca acttcagttt gttggcatgc
     5221 cggccttcgc gagcgactat tctattctgc ctctgaccgg cgcgattgtc ggcggcgtag
     5281 gcacgtttgc cggcgcgatg ctcggcgcct tcctcctcgt gccgatctcg gaaattttcc
     5341 gcgggtttgg aacgtggcgg gtggtggtct actcggtcat cctcgtcgtc tttgtcgtcg
     5401 gcctgccgga gggcctcttt cactatgtgc agcgccggta tcatcagttt gagcgccggg
     5461 tgccctggga aggtgacacg tgagcgccgt tcccatctta aaagtagagc agctcaacaa
     5521 acgcttcggc ggggtcggcg ccgtctataa cgtcagtttc gagctggaag aaggcggcgc
     5581 gttgggcgtc atcggcccga acgggtcggg gaagacgacg ctggtcaatc tcatcaccgg
     5641 ctttgtgcgc ccgacttccg ggcgcgtcct tcttaagggg aaagacatca cccgcctgat
     5701 gccgcacgag cgggttgggc taggcattgc gcgcagtttt cagatggtgc ggccgttcta
     5761 caatctgccc gccttcaaaa acctcatcat ccctttatat tctccgcgtg tcaggagaat
     5821 gaaaggaggc cagtacggca atcgtgatga ggtcgcgatt cacctgctgg aagaagtcgg
     5881 gttcgagcgt gattcgcaag tgccatacaa gaccgccagc aaccttccgc acggctattt
     5941 gaagcgattg gaattggcgc gctgcctcgc gctcgaaccc gaggtcctca tcctcgacga
     6001 attgttctcc ggcatgagct tgtccgaagt cgcgagcctg ctcccgctca tcgaaaaaat
     6061 acgggagcga gggacgacgc tgatgatggt ggagcatcgg ctgcgcgaac tcttccgcat
     6121 tgtcaagaca gtgatcgtgc tgaatttcgg cgagaagatc gccgagggga cgccgtccga
     6181 agtgattgaa atggaggacg tgagaaaggc atacctgggg acaggggtgg gcgccgatgc
     6241 ttgagatcaa ggatctcacc gtctactacg agaatgcgct cgcgctgaac agtctgagcc
     6301 tcgaggtgcg cccaggcgag atcgtcggtg tgttcggatc gaacagcgcg ggcaagacga
     6361 cgcggatgaa tacgattgcc ggcctgacgc tcgacttgaa aaccaaagaa gaacgcaaag
     6421 gcggcgagcg gatcacggtc cacggggagt taaaattcga gggcgaagag atcctgcacg
     6481 ccaagccgag cgaccgcgtg aaaaaaggca tcgtcctgtg ccgcgaacgg catcctgtgt
     6541 tccgcgacag cagcgtcgaa gagaatttga aaatcggcgg attcctgcgc ccctctcgcg
     6601 aggttaagga gcgcatccgc ctggtttacg acatctttcc gcatctgacg cgattaaggc
     6661 gcaggaaggg cgggctgttg agcggcggcg agcagcaaat gttggccgtc gggatggcac
     6721 tcgtcgcgca cccgcgcctg ctgttgctcg acgagccgtt gttgggcctg agtcccctgc
     6781 tgcaggccga catcgtccgt gcgatgagag acattcggca acagggcatc acccttctcg
     6841 tgagtgagca atacgcgcgc cccatctttc cggtcatcga tcgcggctat gtcatcgaaa
     6901 atggcacgct gatcctgacc ggcacgggac cagagctgat gaacaatccc gaagttcgct
     6961 ccgcgtattt tggagtgtag cgatggattc catttatcac gtgtttgaga acaccgcgaa
     7021 cagcctcccg gagaaatcat gcctgatcta tctgggcaag aaatatacct tcgcacaact
     7081 ggccgatgca gttgaccgcg ccgcgacctc tctgacccag tggggggtga agaagggcag
     7141 ccaggcgatt ctttttctgc ccaatttgcc gcagtggatc atcgcctggc tcgccttgca
     7201 aaagatgggc gcggtggcga tcccgattac gcctgtctac gcgccaagcg acctcgagta
     7261 catcgcgaac gacagtggcg cggaaacgat cttttgcttg gacaccaact ttggctatgt
     7321 cacacgtgtc ctttccgaaa cgaagctcaa gcgcgtggtc gtaacgacgc tgatcgaggc
     7381 cttgccgctg tggaagcgag ttctgggccg ggctttgaac aaggttccca aagggatgtt
     7441 ccatctggat gagaacacga tagcgttcag ccagttattg aagactcgcg ggcggtctgg
     7501 cagagagccg gcgccgaacg gggtcaaagg gttggcctct tcaccaggtc tggagatcgc
     7561 cgagatgctt tacaccggcg gcacgaccgg gtatccgaaa ggggtgccga tttctcacgc
     7621 actcttcttg gaagcgatgc gagagcaaag gcgagccggc gaaggtttgg ttcccaaggg
     7681 gcaggacgtg ctcatccagg gcgcgcctct ctaccacatc ctcggccagg tcgtgggttt
     7741 gggagcgctg ctgcacgggg aacagatgat cttgttgccg agacccagcc tcgacgcgac
     7801 gttcgaccac attcagcgct acaaggcgac gacgttcttc ggcgtgccga cgatgtaccg
     7861 gatgattttg gagcacgatc gtgtagacca gtacgatctc ggctcgctca aatattgttt
     7921 ctcgggcggc gatgttctgc cggtggaggt ggcccatcgc tggcgcaaga aattcggcaa
     7981 gccgatccac cagggctacg gcgcgacgga agcgggcggc ggcatttcgt tgacgccggc
     8041 gggcgagcct ttccccgaag gaagcgtggg caaagtcgtt cccttccaaa aaatcaaaat
     8101 cgtgaatccc gatacgctcg agcctctgcc tgtaggagag tcgggtgagt tgctggtttc
     8161 ttcggagcac atggtcaacg cctattggaa caagccggaa gaaaccctgc gcagctttgt
     8221 aaatctcgac gacaaacggt ggtatcgaac gggggacatt gtacgcctcg atgaccaggg
     8281 ttggctgttt ttccgggatc gctcagtgga tgtgatcaaa cacaagggtt accgcgtcgc
     8341 cgcatcgcgc atcgaagccg ttttgcagga acatcccgcg gtgatcgcgt cctgcgtcgt
     8401 cggcgttccc gattcgaatg tcggcgaacg gatcaaagcg ttcgtggttc tcaaggaaga
     8461 tgtgaaaggc attactgcgt acgatctcac cacctggtgc cgcgggcgac tcgcgccgta
     8521 cgaggttccg cactacatcg aatttcgaga tatgctgcct aaatcaaaag tcgggaaact
     8581 gctgaggcgc gaattgaggg cggaagaaca aaggaaaatg gcagcagcct gatttgcacg
     8641 atttatgatg caaacacacc gaggttcaca ggttgcttgc tgcggtgtgc tataatgagc
     8701 ttggggtggg cgagcgatgt gccttggagg caaaggcgat gaaacgcgct ctcactctac
     8761 tgctggctct gatgttcctc gtcggctccg ccgaagggct gccgtcgcgt gcggcggcga
     8821 cagccgcgtg gttgccggtg aacggcccaa cgggcggcag cgtctccgca ctggcgattt
     8881 cccccgatta cgcttacgac cacactgcat acgccggcct gcgcggcagg ggcgtctatc
     8941 gcacggacga tggcggcctg tcgtggaaat cgaccagccc cgacgactgg gtcgtcgtcg
     9001 atctcgcgat ttcgcccgct tacgcggccg atcaggcgct cttcgccgtg accggatttg
     9061 ggacgaccgg ttacaccgtc tatcgctcaa ccgacgcggg cgagacgtgg caggcgccgg
     9121 cgacggtgcc cactggcgga ctgcaaaacc tcaccggcgt tgtcgtctct cccaactacg
     9181 ccgtcgatcg cacactgtac gcgatcggcg cgtcgcaaac atatcgatcg acggacggtg
     9241 gcatcatctt caccgaggcg ggcggctggt tctcgacgca ccctttgacc gacctggcct
     9301 tttcgcccgc gtatgcggcc gatcgcacgc tgtatgccac tgcctcgggc ggcgatccgg
     9361 ccggcctgta caaatcgacc gacggcggga cagtgtggac cccgacgggc ctcaccggca
     9421 atctgagcgc cgtagccgtc tcgccggatt acgccgacga ccagacaatt gcggccatca
     9481 acacggacga tggccgcatc accatctcgc ctgatcgcgg cgcgtcgtgg tcgtccagca
     9541 cgatcacact gtcgggcagc gccgatcaac acacgctgct cttctcgccg acgttcgccg
     9601 ccgatggcat tatgctggcg gcgagcgcgg ccgatccggg cgcctatcgt tctaccaacg
     9661 gcggcgcgac gtggtcgccg gtcggctggt acgactcgac gaagccctac ctgggtggct
     9721 tcgtcggcgg gtcggtcttc gcgctggcgc tcgcgccgac caccgcatca gatgccgccg
     9781 ccttcgccgg gaccagctcc ggcatctatc gctcgtcgaa ctggggcgag cactggtatc
     9841 caggcaacaa aggcttggcc cggctgacca tccgcgcgct cgccgtcgcg ccgaacgatc
     9901 cgcacacgct gctggccggc acggcattct tcgagcgggc gctcacgggc aatcccgacg
     9961 aggatgcggg cagcctgcag ctctccaccg atggcggcgc gacgtggcgc gatgtttcgg
    10021 gccggctcga tcaagttcag caggtggcct tttcgcccga tttcgccgcc gaccggactg
    10081 cgttcgccgt gaccggcctg accgcgccgc ccgacttccg cagcggcggg atctatcgct
    10141 cgacggacgg cgggcagaat tggagcctgg tcagcagcgg gctgatcgga tgggccgtgg
    10201 cgatctcgcc tgacttcgcc gccgatggca cggtgtgggt gtacggttcg agcgcgacag
    10261 agtcgggcat cttcgtttcg gccgacggtg gcgactcgtg ggcgctcctg gcatcttcgc
    10321 cactgcttgc gccccgccca gcggggtctg ccggcgtgat cgctccgtcg ccgaattacg
    10381 ccgtcgatcg gacgatcttc gcgggcacag gcgacggcgg cgtgcagcgc tcgccggacg
    10441 gcgggcagac gtggacgcag gtgttgtccc acacgacgac cgcgctggcc gtgtcgccgc
    10501 tttacgccgc gagccagacg gtatacgcag gtgt
//
LOCUS       DBNHXB010000238        14712 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_393295, whole genome shotgun sequence.
ACCESSION   DBNHXB010000238 DBNHXB010000000
VERSION     DBNHXB010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 14712)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14712)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14712
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_393295"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="AC162G_09870"
     CDS             complement(<1..804)
                     /locus_tag="AC162G_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076165.1"
                     /translation="MSSNGELLLDIQNLRTYFHTRDGIVKAVDGVNFHVNRGEIVGLV
                     GESGCGKSVTSLSILRLVGAPGKIESGRILFDGQNLLELSRDQMTKIRGSRISMIFQQ
                     PTSSLNPVFRAGPQISEVLEIHQAMGRKAGRARAIEMLKKVGIPDPERRVDSYPHEMS
                     GGMAQRVMIAMALACIPDLLIADEPTTALDVTIQAQILDLMRGLQRELGTSIILITHD
                     LGIVAEMCSRVAVMYAGEIVEETDVRTLFRDPKHPYTQGLIESVPMLGIE"
     gene            complement(840..1817)
                     /locus_tag="AC162G_09875"
     CDS             complement(840..1817)
                     /locus_tag="AC162G_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076166.1"
                     /translation="MSESRIAMGSFDVVTEAGTQEAAIQPASLWRDAFHSLLRNRSAQ
                     IGLFILGVLLFVAIFAPLIAPYDPIDYLDPDNTTRTPPCIHLLGCPDDQPQHLFGLDS
                     NGRDLFSRVVYGAQVSLFIGLATVTFGIVVGTLIGAVAGYLGGWFDNSVMRFMDVLLA
                     FPSLLLAIALVAILRPIIVEGGLSPLVPALFAIGFVSIPVYARIVRSSVLSIKEQDYV
                     AADRALGVSRARILFRRIVPNSLTPLIVAGTLGIATAILDAAALGFLGLGQQPPHPEW
                     GAMLGVERASVFNAPHLLIFPGLAIMLTVLAFNLLGDGLRDALDPRLHR"
     gene            complement(1838..2914)
                     /locus_tag="AC162G_09880"
     CDS             complement(1838..2914)
                     /locus_tag="AC162G_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076167.1"
                     /translation="MGSFLLRRLLLIAPVLIGIIFVAFVLVRAIPGDPCTSLLGERAT
                     PQACEDFKERSGLNDNVVVQFLRYLSNLLSGDLGKSIKFGRPIDVILLERMPMTLELA
                     IAALLFAVVFGIPLGIISAYRHNSPIDAATMIGANLGVSMPIFWLGLLLAGLFGVGLK
                     DTPLQLPTQGRLTAGVTIPPLIETWQLGNLEGLPLTIVTFLSNMYTFNALVTGNWTVY
                     GDAVKHLILPAIALGTIPLAIIARLTRSNLLDVMGLDYIRTARAKGVVERAVVFKHAM
                     RNAMLPLVTVIGLQFGALLGGAVLTETVFGLPGMGKFLVDSIFARDYPVIQGFTLVIA
                     LIFVGVNLLVDASYAYLDPRVRLS"
     gene            complement(3012..4739)
                     /locus_tag="AC162G_09885"
     CDS             complement(3012..4739)
                     /locus_tag="AC162G_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5076168.1"
                     /translation="MLHNRKLFAVMSLLMVASVVLSACGGAATTVAPTQPPPPTAVPP
                     TEPPPPTATPPEFEGMAYPEAGPADCAYGGIMKHIEAVDELTVKFTLCEPDPAFRSKV
                     AFSSLGIHSAAQLEATGGGGDLLEQALGTGPYMVEEWVRGDHLTLVANPDFWEEPAKT
                     ERIIFKWNPEATARFTALQSGEADGIDNPDPNDFPAIEGDSSLTLYPRTALNVFYVGM
                     NNFFPPFDDERVRQALAMGIDRQAIVDKYYPPGSEVASHFTPCAIPGGCEGESWYEYD
                     PEGAKALLAEAGYADGFDVTVSYRDVVRGYLPAPGKVAEEIQAQLKEYGINVTIEVQE
                     SGTFITNAGEGKLPFFLLGWGADFPDMVNFVDVFFGAGASPRFGDLYPDIVEVLAEAR
                     TLADQGERNKLYAEANTLIKQHSPMVAVAHGGSATVFKADVEGAHSSPLSTEIWRVLE
                     APDRDTLVWMQNAEPLSLYCADETDGETLRACEQIFDALLSYKIGSTEVEPGLAESCD
                     PNADLTEWTCNLRPGVTFSDGTPLTANDVVATFAVQWDAAHPLHVGNSGLFEYWTFLF
                     ASFLNPPPQ"
     gene            complement(4956..5321)
                     /locus_tag="AC162G_09890"
     CDS             complement(4956..5321)
                     /locus_tag="AC162G_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR5076169.1"
                     /translation="MNGAVAQEILEADIVRVAPQGRLDTFSVPPFEQVLTEHLDAGRA
                     RLIVDLAGVTYVSSSGLRALLSARRRARLAGGDVVLCSMAPRVREIFEMVGFISLFNV
                     YPTAEEAIRSVREGIPPRA"
     gene            complement(5318..5752)
                     /locus_tag="AC162G_09895"
     CDS             complement(5318..5752)
                     /locus_tag="AC162G_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5076170.1"
                     /translation="MSEEKTLRVPGRFESLAEIADFIAGVARDAGFDEDDVFRVQMAV
                     DEACSNVVEHAYGPNRVGDIVLSCRVDGNGDLRVDIYDRGRPFNPDVVPEPRVGANLL
                     DLEEMKVGGLGLFFMRKLMDEVTFHFDEGVGNHLSMVKRGAK"
     gene            complement(5760..6092)
                     /locus_tag="AC162G_09900"
     CDS             complement(5760..6092)
                     /locus_tag="AC162G_09900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR5076171.1"
                     /translation="MQISSRDLKRVNVVTVSGRVDSAASPDLEKALQDLLESRRHQIV
                     MELAEVEYMSSAGLRALVSALKAAKKAGGDVVIARPSERVREVIDLAGLTSVFTMYED
                     TLEAVGSF"
     gene            complement(6263..7702)
                     /locus_tag="AC162G_09905"
     CDS             complement(6263..7702)
                     /locus_tag="AC162G_09905"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR5076172.1"
                     /translation="MQRTRWFQSVFRPTDARLSMPSVLILVLAVSLVGGLLVSLLGGL
                     ISLGLLAGMTLGLWVLRDIDVGYVALIAVICLLPFGALPVNVGFKPTFLDLIVGALFF
                     VWLMQRVTGKLGRFAAVPIGVPVLIFLALALASFVAGLGHAPLTQTVARHFAEVLLSI
                     MLFFMVVDTVRDEARLAKFVRAIMIAGFLASLIAIVLYILPDGTTMELLSRLRVFGYP
                     EGPGVLRYIRDDPSLTQRATGTAVDPNVLGGLLIMTLSVSAAQVFGSRPVVRRGWAAI
                     IAAAMATALVLTISRGSFVGAGAALLGLSVLRYRKIMPILAVAVIFILILPQTQAYVT
                     HFFEGVQGEDLATQMRFGEYRDALTLIGRYPIFGVGFTGAPDLDTYLGVANVYLTIAQ
                     QMGMVGLAAFLLAMLTLFAWSWRARRAVRDRPALEPMWWGLHAAIAGALVGGIFDHYF
                     FNLDFHHSVAFFWLFAGLAAGATRLASNQ"
     gene            complement(7764..9707)
                     /locus_tag="AC162G_09910"
     CDS             complement(7764..9707)
                     /locus_tag="AC162G_09910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR5076173.1"
                     /translation="MREAIPARLTLRPVSVRPTFLAVLICLAFLAVWIWDLGYPSLSS
                     DESFVAILTTRPAGEILRLLNTDEPHPPVYYLIMRAWHILAGSRPEFLVRYLSVLNGV
                     LLLSLVYRLGRTIGLGQLAALSAVLFLGLNPQVTAHVREARMYGPMITGLAFAVLIGL
                     RFERLPRRSAIGIAAVASLLALLTHYFNVLFVAALGLWGLMALTGAARRRWLVSQVIA
                     WGAFALWLPLMGQGFFNPTSLSAGKTWSFTLLPWDTLGRLAVVGTFGYRDIPDVWPSV
                     MGGALLVGGWLAGSLQAAKPWRWLLLGMVALPLGGYAVLVWVKPLYHPKYMLPWLLFA
                     VLGLGSLVGRRRWLGLGVFFVLAAVMLSPTWRTIRLPYDPRFSMSPNEWLRPLPRQLS
                     AYLVEHAGSRDVFGLGTPDAAHCYYSDVYFERSLGCALIPAYPTQTLEELEEQLEALL
                     AEHEVLWYLDYHNPAWDPQRIAEAALARLALHLGPADANGNRLRLYASPATILRRQQA
                     VGARFGEVAELEGVWIRRGTDLHIVLIWRALADRPHIGAKVFVHLLDVSGRLVAQADG
                     VPVWWTRPLETWRLGEQLLDVYTLSLPEGIHLDEGKLRIGLYDPDTVTRLPARDQGGN
                     RLPEDAASLPLVAWFPPASTADP"
     gene            complement(9797..11692)
                     /locus_tag="AC162G_09915"
     CDS             complement(9797..11692)
                     /locus_tag="AC162G_09915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR5076174.1"
                     /translation="MSGEGAASSARERDMLPVLLGVILLAFALRMFQIDAQELRGDEA
                     FSWSYVTGEPDPIALVARIVREGDPQPPMHYWLLQIWMRVFGDGEFALRSLSGFLSLL
                     LVPLIFKLAHRVDEDDWLALSAAGIAAVNPYQIWLAQDVRSMYLLALIGLVVATRQLP
                     GVLCPGRRSWALYVLSASFALYSHYYAIFGLLAHGLYVALKRPARPGWLRWLGAGGAI
                     ALVVLPWWITMLPIYSAGQLADPAIYPVADFLLETLGDAGLGPAVPDPVALAGMLIST
                     VAVLAGAMWLWPRRRPWAGLLLGWPLITLVGIYLVTRTRATYNTFYFSVAFPALYILF
                     AAALAMLWQRPRRVALVALCGLALIGCAASLYNYHFDPHWSKSRGLRDLAALLATDSR
                     PGDVIVANLPDPATVYYLRRLTLPYDMLPAEPDFDPVEVDGAVGALASRYDRIWHIPM
                     RVAQWDADGFVESRLADRYFAAADYRFGKTRLRLFVPRPEDLPYYRSLNVEFAQGIRL
                     VGAYVTVNGNPNYPHPKGGDWLRVSLIWTTTTAIPADYTVFVHALSSEGGIAGQHDSI
                     PRNSAAPTRAWRPGAIVLDVHEFQLPPESAVLDRIVVGMYDPTTGRRLSLLEGGDSVL
                     VWQPASQ"
     gene            complement(11682..13685)
                     /locus_tag="AC162G_09920"
     CDS             complement(11682..13685)
                     /locus_tag="AC162G_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076175.1"
                     /translation="MYSGHPFLADVQSASFYPPRWITILLAGPGGFTYFALEMEAIAH
                     VFLAGAFIFAFAWRVLKDRIAALVAAIVFAFGGYLTSYPILQLAILETVTWLPLLLLL
                     VDIAFTSARAGSRSTASVGAGLVMAIALLAGHPQTALHMAYLALLYGVWRAYSQRTGW
                     REAAARFAVLGLIGLGASAAQWLPSLEFMRLSTRSELGYDALAGGFALRDIVQMLLPG
                     TTGAWSPLYVGLVPLLLAVFAVLGDGKRLAFFWLAIALGAFVLSLGGATFLYNLLYQW
                     VPGFNLFRSQERAAGIMSFALAIAAGIGFVALRAQDSQRLAGLMRRALGWLVAGLVLL
                     AAVLFALWQAAYARGAPDVTTALDRTVFAAFLALASIGWLKLRQPPSTGRPLWPALAL
                     ALIVFDLFTTNWRTDLEPRLPELQAALPGLLDTVRADADLARVDDRGWLEGNWGNLAH
                     VQSVNGASPLVIGHYARVRDALTKEREWELFNVKYAIIDQDSLSVPAEVVDSRSGLGR
                     RLNLFRLADPAPRVWMVHRARVMDDDSALAALADVAFDLHREAIVAEPVTVTESSAGS
                     SLRVSTYGPERIVVDVDASAAGLLMLSELDYPGWRATLDGAETPVVRADVALRSVVVP
                     VGHHRVEMVFQPSSVWIGVGISGVTCFAGLIVLARSMRRRHER"
     gene            complement(13901..>14712)
                     /locus_tag="AC162G_09925"
     CDS             complement(13901..>14712)
                     /locus_tag="AC162G_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076176.1"
                     /translation="RRVDSDGQLRVTLYWQCLVPIQDNYSVSISAKGRDLAEIGKIDA
                     YPHRGLLATSDCPAGAIFADEYRIPLDPDAQRPTLVRAHINLIDWSRRRWAALTDGAG
                     NPIPALIFDASALPSPVTWPAKSPTEGRREPAVSSRITFGDVIQLDGYELNVASSASK
                     QVALGLHWSALQRPPEDYTVFVHLLDSQGRMITQWDSQPLNGDYPTSWWQPGETVLDT
                     RILPLPGDLPAGAYRLALGLYRIDDGARLPVRDASQNPMPDDRVLIGISLP"
BASE COUNT         3118 a         4789 c         4481 g         2324 t
ORIGIN      
        1 ctctatgccc agcatgggaa ccgactcgat gaggccctgc gtgtagggat gcttcgggtc
       61 gcggaacagg gtgcgcacgt cggtttcctc gacgatctcc ccggcataca tcaccgccac
      121 gcggctgcac atctcggcga cgatgcccag gtcgtgggtg atcaggatga tcgaggtgcc
      181 gagttcgcgc tgcaggcctc gcatcaggtc gaggatctgc gcctggatcg tcacgtcgag
      241 cgcggtcgtc ggctcgtcgg cgatcagaag atcgggaatg cacgccagcg ccatggcgat
      301 catcacgcgc tgggccatgc cacccgacat ctcgtgcggg tacgagtcga cgcggcgctc
      361 cggatcaggg atacccactt tcttcagcat ctcgatcgcg cgcgcgcgtc ccgccttgcg
      421 gcccatggcc tggtgaatct ccagcacttc gctgatttga ggccccgccc ggaaaaccgg
      481 gttcaacgat gaggtgggtt gctggaagat catcgagatg cgcgagccgc gaatcttggt
      541 catctgatcg cgcgacaatt ccagcagatt ctgcccgtcg aataggatgc gacccgattc
      601 gatcttgccc ggcgctccga ccaggcgcag gatcgacagc gaggtcacgc tcttgccgca
      661 gcccgactca ccgaccagcc cgacgatctc gccgcggttg acgtggaagt tcacgccatc
      721 caccgccttg acgatcccat cacgggtatg gaagtaggtg cgcaggtttt gaatgtcgag
      781 gagaagctcg ccgttagaag acatagctgc gatgatcggt cggctacgcg cacaagggat
      841 cagcgatgta gacgcgggtc gagcgcatcg cgcagaccgt cgccgagcag attgaaggcc
      901 agtacggtga gcatgatcgc caggcccgga aagatcagaa ggtgcggcgc gttgaacacg
      961 ctggcgcgct cgacgccaag catcgcgccc cattcaggat gcggcggctg ctggcccaat
     1021 cctaggaatc cgagcgccgc cgcgtcgagg atcgccgtcg cgatgcccag cgtgccggcg
     1081 acgatgagcg gcgtgagtga gttgggcaca atgcgccgga acaggatgcg cgcccgcgac
     1141 acgccgagcg cacggtcggc ggcgacgtaa tcctgctcct tgatcgacag cacactcgac
     1201 cgcacgatgc gcgcatagac gggtatagac acgaagccga tagcaaacaa tgccgggacg
     1261 agcggcgaca ggccgccctc gacgatgatc gggcgcagga tcgctaccag cgcaattgcc
     1321 agcaacaggc tggggaaggc cagcaggacg tccatgaagc gcatcaccga gttatcgaac
     1381 cagccgccga ggtagccggc cactgcgccg atcaacgtgc cgaccactat gccgaacgtg
     1441 accgtcgcca ggccgatgaa gagcgacacc tgcgcgccgt acacgacccg gctgaacaaa
     1501 tcgcggccgt tgctgtccag cccgaaaagg tgttgaggct gatcgtccgg acagcccagc
     1561 aggtggatgc acggcggcgt gcgcgtcgtg ttgtccggat cgaggtagtc gatcggatcg
     1621 tacggcgcga tcagcggagc gaaaatcgcg acgaacagca gaacacccag aataaacaat
     1681 ccgatctggg ccgatcggtt ccggagcagg ctgtggaagg cgtcgcgcca tagactggcc
     1741 ggctggatcg ctgcttcctg agtccccgcc tcggtcacaa cgtcgaagga ccccatggcg
     1801 attctcgatt cactcatcac gcgccaccta tcacgtatca gctcaacctc acgcgcgggt
     1861 cgaggtaggc gtacgaagca tcgaccagca aattgactcc gacgaagatc agggcgatga
     1921 caagcgtgaa gccctggatg accggataat cgcgcgcgaa aatcgagtcc accaggaact
     1981 tgcccatgcc gggcaggccg aataccgtct cggtcagcac ggcgccgccg agcagcgcgc
     2041 cgaactgcag gccgatgacc gtcaccagcg gcagcatcgc gttgcgcatg gcgtgcttga
     2101 acacgacggc ccgttcgacg acgcccttgg cgcgcgcggt gcggatgtag tcaagcccca
     2161 tcacgtcgag caagttggag cgggtgagcc gggcgatgat cgcgagcggg atcgtgccga
     2221 gcgcgatggc aggcaggatg agatgcttga cggcatcccc atagacagtc cagttgccgg
     2281 tcaccagcgc attgaaggtg tacatgttgg agaggaaggt gacgatcgtg agcggcaggc
     2341 cttcgaggtt tccgagctgc cacgtttcga tcagtggcgg aatcgtgacg cccgccgtca
     2401 accggccctg cgtcggcaac tgaagcggcg tgtctttcag gccgacgccg aacaagccgg
     2461 ccaggagcaa gccgagccag aaaatcggca tcgacacgcc gaggttggcg ccgatcatcg
     2521 tggcggcgtc aatcggcgag ttatgacgat aggctgagat gatccccaac ggaataccga
     2581 acacgacggc gaagagcaag gccgcgatcg ccagctcgag cgtcatcggc atgcgttcca
     2641 gcaggatgac gtcgatgggc cggccgaact tgatcgactt gcccagatcg ccgctcaaca
     2701 ggttcgacag gtagcgcaga aactgaacga cgacgttgtc gttgagcccg gagcgttctt
     2761 tgaaatcctc gcaggcctgc ggcgtggcgc gctcgcccag cagcgacgtg cacggatcgc
     2821 cggggatggc gcgcacaagg acgaaggcaa cgaaaatgat cccgatgaga acgggggcga
     2881 tcaacaacag gcgccgcaac aggaagctac ccattcggct ctcacactct tgaccgatta
     2941 tggcgggtga gcggggctat gcccactcac ccgcctatct ccctgcactt acccctacct
     3001 gcgccgcgtg atcactgcgg cggcgggttc aggaacgaag cgaacaggaa cgtccagtac
     3061 tcgaacaggc cggagttgcc gacgtgcagc ggatgagcgg catcccactg aacggcgaac
     3121 gttgccacca catcgttcgc cgtgagcggc gtgccatccg aaaacgtgac gcccgggcgg
     3181 agattgcacg tccactcggt cagatcggcg ttcggatcgc agctctcggc caggccgggt
     3241 tcgacttcgg tgctgccgat cttgtacgac agcagcgcgt cgaagatctg ctcgcaggcg
     3301 cgcagcgtct cgccgtcggt ctcgtcggcg cagtacaggc tgagcggctc ggcgttctgc
     3361 atccacacca gcgtgtcgcg gtccggcgct tccagcacgc gccagatctc ggtgctcagc
     3421 gggctggagt gtgctccttc cacgtcggcc ttgaagactg tcgccgaacc gccgtgagcc
     3481 acggcgacca tcggcgagtg ctgcttgatc agcgtgttgg cctcggcata caacttgttg
     3541 cgctcccctt gatcggccag cgtgcgcgcc tcagccagca cctcgacaat atccgggtac
     3601 aggtcgccga agcgcgggct ggcgccggca ccaaagaaca cgtccacgaa gttcaccatg
     3661 tccgggaagt ctgcgcccca accgagcagg aagaagggca gtttgccctc gccggcattc
     3721 gtgatgaacg tgcccgattc ctgcacctcg atcgtaacgt tgatgccgta ctccttgagc
     3781 tgcgcctgta tctcttccgc caccttcccg ggcgcgggca ggtagccgcg caccacatcg
     3841 cggtaggaca ccgtcacgtc gaatccatcg gcgtagccgg cctcggccag cagcgccttg
     3901 gctccctctg gatcgtactc ataccaggat tcgccctcgc agccgccggg aatggcgcac
     3961 ggcgtgaagt gcgaagcgac ctctgagccg ggcgggtagt atttgtcaac gatcgcctgc
     4021 cggtcgatgc ccatcgcaag cgcctggcgg acgcgctcgt cgtcgaaagg cgggaaaaag
     4081 ttgttcatgc cgacatagaa cacgttcagc gcggtgcgcg gatagagcgt cagcgaggaa
     4141 tcgccctcga tcgccgggaa gtcgttcgga tcggggttgt cgatgccgtc ggcctcgccc
     4201 gactgcagcg ccgtgaagcg cgccgtcgcc tccggattcc acttgaagat gatgcgctcc
     4261 gtcttggccg gctcctccca gaagtccggg ttggccacca gcgtcaggtg atcgccgcgc
     4321 acccattcct cgaccatgta cgggccggtg ccaagcgcct gctccagcag gtcgccgccg
     4381 ccgccggtcg cttccaactg cgcggcagag tgaataccca acgatgagaa ggcgaccttc
     4441 gaccggaagg ccggatcagg ctcgcacagc gtgaacttga ccgtcagctc atccacggcc
     4501 tcgatatgct tcatgatgcc gccgtaagcg cagtctgccg gccccgcctc agggtaggcc
     4561 atgccctcga actcgggcgg cgttgcggtg ggtggcgggg gctccgtggg tggcaccgcg
     4621 gtgggcggcg gcggctgagt cggcgcgacc gtcgtcgccg cgccgccgca ggccgataaa
     4681 acgaccgaag cgaccatcag cagcgacatc actgcaaaca gcttgcggtt gtgcaacatt
     4741 ctttctcctc cttagagaaa acttgcgtgt agagacgttg taccgacaag cgaaccagaa
     4801 tgactctccc tggcctcacc tcccttctaa tgcatcttgc gccttgaccg tgccggaata
     4861 cttaccaatc cgtgtcatcc gcctgccctc gcctgctgcc tgccctcgcc cgctgtctgc
     4921 gggggtgtgc gggggtgaga aatctgtgtg tcaggtcatg cgcgcggcgg tatgccttct
     4981 cggacggatc ggatggcctc ctcggcggtc ggatatacgt tgaacaggct gatgaagccc
     5041 accatctcga agatctcgcg cacgcgcggc gccatgctgc acaggacgac gtcgccgccg
     5101 gccaagcgcg cgcgccgccg cgcgctcagc agcgcgcgca ggccgctgct cgacacgtac
     5161 gtcactcccg ccagatccac gatcagccgc gcgcgaccgg cgtccaggtg ctccgtcagg
     5221 acctgttcga acggcggcac gctgaacgtg tccaaccgtc cctgcggggc aacgcggacg
     5281 atatcggcct cgagtatttc ctgcgccacg gcgccgttca cttggcgccc cgcttcacca
     5341 tgctcagatg attgcccacg ccctcgtcga aatggaacgt cacttcgtcc atcagtttgc
     5401 gcatgaaaaa caacccaagt ccgccgacct tcatctcttc gagatccaac agattggcgc
     5461 cgacccgtgg ttccggaacc acgtccggat tgaacggccg gccccggtcg tagatgtcta
     5521 cgcgcaaatc gccgttgccg tcgacgcggc aggataagac gatgtcgccc acccgattcg
     5581 gaccgtacgc gtgctcgacg acattggagc acgcctcatc gacggccatc tggacgcgaa
     5641 agacgtcatc ctcgtcgaag ccggcatccc gggcgacccc agcgatgaag tctgcgatct
     5701 cggccaggct ctcgaagcgg cccggcactc tgagggtttt ctcttccgac atcgaaggct
     5761 caaaatgagc cgactgcctc cagagtgtcc tcgtacatgg tgaagaccga cgtcagcccg
     5821 gcgagatcga tcacttcgcg cacgcgctcc gagggccggg cgatcaccac gtcgcccccc
     5881 gccttcttgg ccgctttgag cgcagacacc aacgcgcgca gacccgcgct gctcatgtat
     5941 tcgacttcgg ccagctccat gacgatttga tggcgccggc tctcgagcag gtcttgcagc
     6001 gccttttcca gatccggcga tgccgcgctg tcgacgcggc cgctcaccgt aacgacgttg
     6061 acacgcttca aatcgcggct agagatctgc atccctcgac tcctgtttag ggtgtgagcg
     6121 attataccac aggcattttg caacggccaa agttatcagt catcagttgt cagtcatcag
     6181 ttatcagtgg tcagtggtca gtcatttcgc tactcactga tcactgctca ctgatcactg
     6241 attactgatc actgattact gctcactggt tactagccag tcgcgtggcg ccggctgcca
     6301 ggccggcgaa cagccaaaag aaggcaaccg agtggtgaaa gtccaggttg aagaaataat
     6361 ggtcgaatat gccgccgacc agcgcgccgg cgatcgcggc gtgcagcccc caccacatcg
     6421 gctccagcgc tgggcggtcg cgcacggccc gacgcgctcg ccacgaccac gcgaacagcg
     6481 tgagcatggc gagcaggaag gcggccaggc cgaccattcc catttgctga gcgatcgtca
     6541 ggtacacatt ggcgacgccg aggtaggtat cgaggtcggg agcgccggtg aagccgacgc
     6601 cgaagatggg atagcgtccg atcagggtca gcgcgtctcg atactcgccg aagcgcattt
     6661 gcgtcgccag gtcttcaccc tgcacgcctt cgaagaagtg cgtgacgtag gcctgcgtct
     6721 gcggcaagat gagtatgaat atgaccgcca cagccaggat cggcatgatc ttgcggtagc
     6781 gcaacacact caacccgagc agcgcggcgc cggcgccgac gaacgatccg cgcgagatgg
     6841 tcagcaccag cgcagtcgcc atcgccgcag cgatgatcgc cgcccagccg cgtcgcacga
     6901 ccggccgcga gccgaagacc tgcgccgcgc tcacggagag cgtcatgatc agcagccccc
     6961 cgagcacgtt cggatcgacc gcagtgccgg tcgcgcgctg cgtcaggctt ggatcgtcgc
     7021 ggatgtagcg cagcacgccc ggcccttccg ggtaaccgaa aacgcgcagc cgggacagca
     7081 gctccatcgt cgtgccatcg ggcaggatgt acaacacgat cgcgatgagc gacgccagga
     7141 agccggcgat catgatcgcg cggacgaact tcgccaggcg cgcctcgtcg cgaaccgtat
     7201 ccacgaccat gaaaaacagc atgatgctga gcaagacctc cgcgaagtgg cgcgccacgg
     7261 tctgcgtcaa gggcgcgtgc cccagcccgg ccacgaagga cgccagcgcc agcgccagga
     7321 agatcagcac cggcacgccg atcggcacag cggcgaaccg gcccaatttg cccgtcacac
     7381 gctgcatcag ccacacgaag aacagcgccc cgacgatcag gtcgaggaac gtcggcttga
     7441 acccgacgtt gaccggcaac gcgccgaagg gaagaagaca aatgacggca atcagggcca
     7501 cgtagccgac gtcgatgtcg cgtagcaccc acaggccgag ggtcatgccg gcgagcaggc
     7561 cgaggctgat cagcccgccc agcagtgaga cgagcagccc gccgaccagc gagaccgcca
     7621 gcaccaggat cagcacgctc ggcattgaca gccgtgcgtc agtgggtcgg aacacagatt
     7681 gaaaccaacg tgtgcgctgc atatacaaat taaactcctg gaatgggcaa aaggttcagt
     7741 ggcgtctgac cggcacacgg cgatcacgga tcggctgtgg atgcgggtgg gaaccaggcg
     7801 accaggggta atgaggctgc atcctcagga agccggtttc ccccttgatc gcgcgccggc
     7861 agccgggtga ccgtatctgg atcatacaga ccgatgcgga gcttcccctc atccaggtga
     7921 ataccctccg gcaaagatag cgtgtaaacg tccaagagtt gctcgcccag gcgccatgtc
     7981 tccaaagggc gggtccacca cacgggcaca ccgtcagcct gagcgacgag ccggccggat
     8041 acgtccaaca agtgaacgaa gactttagcg ccgatgtgcg gacgatcggc cagagcgcgc
     8101 cagatgagca cgatatgcaa gtcagtgccg cgcctgatcc aaacgccttc cagttcagcc
     8161 acctccccga accgcgcgcc gaccgcctgc tggcggcgca ggatggtcgc cgggctggcg
     8221 tacaggcgca gccgattgcc attcgcgtcg gccgggccca ggtgcagcgc cagtcgagcc
     8281 agcgccgcct cggcgatgcg ctgcggatcc caggccgggt tgtggtagtc cagataccat
     8341 agcacctcgt gctcggccag cagcgcttca agttgttcct ccaactcttc gagtgtctgt
     8401 gtcgggtagg ccgggatcag tgcacagccc aggctgcgct caaagtagac atcggagtag
     8461 tagcagtgcg ccgcatcggg cgtacccagc ccgaacacgt ccctcgaccc ggcgtgctcc
     8521 accagatacg cgctcaattg gcgaggaaga gggcgcagcc actcgttggg ggacatcgag
     8581 aatcttggat catatggcag gcgtatcgtg cgccaggtag gtgagagcat caccgcggcc
     8641 agcacgaaga acacccccag ccccagccag cgccgacgcc ctaccagact ccccaacccc
     8701 aacaccgcaa acagcagcca cggcagcatg tacttcgggt gataaagcgg cttgacccac
     8761 actaacaccg cataccctcc taacggcagc gccaccatcc ccagcagcag ccagcgccag
     8821 ggcttcgcag cctggagact ccctgccagc cagcccccta ccagcaacgc cccacccatg
     8881 acgctcggcc acacgtctgg gatgtcccga tacccaaatg tccccaccac cgccagacgc
     8941 cccagggtgt cccacggcag cagcgtgaac gaccacgtct tgcccgcgct caggctcgtt
     9001 gggttgaaaa agccttgccc catcagcggc agccaaagcg cgaaagcccc ccaggcgata
     9061 acctgcgaaa cgagccagcg gcggcgggca gcgccggtca acgccatcag tccccatagg
     9121 cctagcgcag ccacaaatag aacattaaag taatgcgtca gcaaggccaa caggctggcc
     9181 acagccgcga tcccgatggc tgagcgtcga ggcaaccgct cgaagcgcag cccaattagc
     9241 accgcgaagg ccagaccggt gatcatcggg ccgtacattc gcgcctcgcg cacatgcgcc
     9301 gtaacctgag gattcaagcc caggaagaga acggccgata atgctgcgag ctgtcccaga
     9361 ccgattgtgc gtcccaggcg gtagaccagg ctaagcaata atactccgtt cagcactgac
     9421 aggtatcgta cgaggaactc cggacgactc cccgccagga tgtgccaggc acgcatgatg
     9481 agataataga ccggcggatg tggctcatcg gtgttcagca gtcgcaatat ctcaccggcc
     9541 ggccgggtgg tcaaaatcgc cacgaacgac tcatcactgc tgagcgacgg gtaacccagg
     9601 tcccagatcc agactgccag gaaggcaagg cagatcagga cagccagaaa agtcggcctt
     9661 acgctgacag ggcgcagtgt cagccgtgca gggattgctt cacgcatgag cctcgatcta
     9721 ctccgtgagt gtcacgggca tggagatgct cgtaccgtgc cactgaaagg ttgacctcgt
     9781 tcaaacacgg ccaggtttat tgagacgctg gctgccagac cagcacactg tcgccacctt
     9841 ccaaaagact gagccgtcgt ccggttgtgg gatcgtacat gcccacgacg atccgatcca
     9901 gaacggcgct ctccggcggg agctgaaatt cgtgcacatc gagaacgatc gcgccaggcc
     9961 gccaggcacg ggtgggcgct gcgctgttcc gcgggatgct gtcgtgctga cctgcgatcc
    10021 cgccctccga tgagagcgcg tgcacgaaga ccgtgtaatc ggcggggatg gcagttgtcg
    10081 ttgtccatat gaggctaacc cgcagccaat ctcctccctt agggtgagga taattcgggt
    10141 tgccgtttac ggtgacgtac gcccccacca atcttatccc ctgtgcaaac tcaacattca
    10201 gcgatcgata gtatggcaag tcttccgggc ggggcacgaa cagccgcagc cgtgttttgc
    10261 caaatcggta atctgctgca gcgaagtaac ggtccgcaag ccgcgattcg acaaaaccat
    10321 cagcgtccca ttgagccacg cgcatcggta tatgccatat tcggtcgtat cgggacgcga
    10381 gtgcgccaac tgcaccgtcc acctccactg gatcgaagtc gggctcggcc ggcagcatat
    10441 cgtatggcag tgtgaggcgc ctcaagtagt acacggttgc aggatctgga agattcgcga
    10501 cgatgacgtc gcccggccgg ctgtccgtgg cgagcagagc ggcgagatct cgtagtccac
    10561 gcgacttgct ccagtgcgga tcgaaatgat agttatacag gctcgccgca catccaatca
    10621 gtgccagtcc acatagagca actagggcga cacggcgggg acgctgccac agcatcgcca
    10681 gcgcagcggc gaacaggatg tacagagcgg gaaaagctac gctgaagtag aacgtattgt
    10741 aggtcgcgcg ggtccgtgtc actaaatata taccgaccaa cgtgatcaac ggccagccca
    10801 acagcagccc ggcccagggc cggcgcctgg gccagagcca catcgcgccg gccaagaccg
    10861 caaccgtact gatgagcatt cccgccagcg caacgggatc gggcaccgcc ggccccaagc
    10921 ccgcgtcgcc cagagtctct aatagaaagt ccgccacggg gtaaatcgcc gggtcggcga
    10981 gctggccggc gctgtagatc ggcagcatag ttatccacca tggtagcacg acgagcgcga
    11041 ttgcgccgcc ggcgcctagc caccgcagcc agccgggtcg tgcggggcgc ttcagcgcga
    11101 catacaggcc atgcgccaac agcccgaaaa tggcgtagta atggctgtac agcgcgaacg
    11161 atgcgctcag cacatagagc gcccaactgc gccgaccggg gcacagcaca cctggcaatt
    11221 gtcgcgtggc gacaaccaac cctatcagtg caagcaggta catgctgcgc acatcctgcg
    11281 ccagccagat ctgatacgga ttcacggcgg ctatgccggc cgcgctgaga gccagccaat
    11341 cgtcttcatc cacccggtgg gcaagtttga agatcagcgg caccagtaaa agactgagaa
    11401 aacccgacag actgcgcaac gcaaactcgc catcgccgaa aacgcgcatc catatctgca
    11461 atagccagta atgcatgggc ggttgaggat cgccttcacg cacgatgcgc gcgaccagcg
    11521 cgatcggatc cggctcgccg gtgacgtagc tccaactgaa tgcttcatcg ccccgaagtt
    11581 cctgagcgtc gatttgaaac atgcgaagcg cgaacgccaa cagaatgacg ccgagcaaca
    11641 cgggcagcat gtcgcgctcg cgcgcggaag aagccgcgcc ttcaccgctc atggcgcctc
    11701 cgcatagacc gtgcgagcac gatcaatccg gcgaagcacg tcacacccga gataccgaca
    11761 ccgatccata cgctggaagg ctgaaagacc atctccacac gatgatgtcc caccggtacc
    11821 accaccgagc gcaacgcgac atctgcccgc acgacgggcg tctcggcgcc atccaatgtc
    11881 gcacgccaac cgggatagtc tagctcgctc aacatcagaa ggccggctgc cgatgcgtcg
    11941 acgtcgacca caatgcgctc cggcccatag gtcgagactc gcagcgagga accggcggac
    12001 gattcagtta ctgtcacagg ctctgcgacg attgcctcgc gatgcagatc gaacgccaca
    12061 tcggccaatg cggccagcgc cgagtcgtca tccatcacgc gagcgcgatg caccatccac
    12121 acgcgaggcg ccgggtcggc caggcggaac agattcaagc gtcggccaag cccactcctg
    12181 ctgtcaacga cttcagccgg gacagacagg ctgtcctggt cgatgatggc gtacttcacg
    12241 ttgaacaatt cccactcccg ctcctttgtc aacgcatcac gcacgcgcgc ataatgcccg
    12301 atcaccagcg ggctggctcc attcacggac tgcacatgcg ccaggttgcc ccagtttccc
    12361 tcaagccagc cgcggtcgtc tacgcgggcg agatcggcat ccgcgcgaac ggtgtcgagc
    12421 agaccgggaa gagcagcctg cagctcgggc agacgcggct caaggtccgt gcgccagttg
    12481 gtcgtgaaca ggtcaaacac gatcagcgcg agcgcgagcg ccggccataa cgggcgtccc
    12541 gtcgatggag gttggcgcag cttcaaccag ccgatcgagg caagtgccag gaaagccgcg
    12601 aacacggttc gatccagcgc cgtcgtcaca tcgggcgcgc cacgggcata ggccgcctgc
    12661 cacagcgcaa acagaaccgc tgccaaaagg actaaaccgg caacgagcca gcctagagcg
    12721 cgtcgcatca agccagccag gcgctgcgaa tcctgcgcgc ggagcgctac gaatccgatt
    12781 ccggcggcga tcgcaagtgc gaaactcatg atgcccgcgg ctcgttcctg tgatcgaaaa
    12841 aggttgaacc cgggcaccca ctggtacagg aggttataca gaaacgtcgc gccgccgaga
    12901 gacaatacaa acgcacccag cgcaatggcc aaccaaaaga atgccagtcg cttgccatcg
    12961 cccagcaccg cgaagacggc aaggagcaag ggcacgaggc ccacgtacag cggcgaccat
    13021 gccccagtcg tacccggcag cagcatctgg actatgtcgc gcagggcgaa gccccccgcc
    13081 agcgcgtcat atcccaactc actccgcgtg gacagtcgca tgaactcaag cgacggcagc
    13141 cactgcgctg ccgacgcgcc tagtccgatg agtcccaaga cggcgaaccg tgccgccgct
    13201 tcgcgccagc cggtgcgctg cgagtacgcg cgccacacgc catacagcaa agccaggtag
    13261 gccatgtgca gcgccgtttg gggatgccct gccagcagcg caatcgccat gacgagcccg
    13321 gcgccgacac tcgccgtgct gcgcgaccca gcccgcgccg aggtgaaggc gatatcgacc
    13381 aggagcaaga gcagcggcaa ccaggtcaca gtctcaagga tggccaattg aagaatggga
    13441 tagctggtca ggtaaccgcc gaaagcgaag acgatggctg cgacgagcgc cgctatccgg
    13501 tctttcagca cacgccacgc gaaggcgaag atgaaagcgc cggccaggaa gacgtgcgcg
    13561 atcgcttcca tctccaacgc gaaatatgtg aagccacccg ggccggcaag caggatggtg
    13621 atccagcgcg gcggatagaa gctggccgac tgcacgtcgg ccaggaaagg atgccctgag
    13681 tacacgtacg gattccacaa tggaagttga ccttgccaga ggcgctcgga ctggtaaact
    13741 gcggacgcgt agaactgtcc ggagaaatcg cccgccgcaa acgaggcgcg atcaatcgca
    13801 ctgggcgtcc agatacgcca aaagaacgca gcgcacagaa ggcctaggaa cccataggga
    13861 gcagctccgc gcagaacagg cttcaatcgg tcaaacatgg tcagggaagt gatatgccga
    13921 tcagaacacg atcgtctggc attggattct gactcgcatc acgaacgggc agacgcgccc
    13981 cgtcatctat gcgatacagg ccgagtgcca gtcgataagc gccggccggc agatcacccg
    14041 gcagcggcag aatacgcgtg tccaacaccg tctcgccggg ctgccaccac gaggtaggat
    14101 agtcgccatt cagcggctga ctgtcccatt gtgtaatcat acggccctgg ctgtcaagta
    14161 gatggacgaa caccgtgtaa tcctcaggag gacgctgcaa ggcagaccag tgtagcccca
    14221 acgcgacttg cttcgacgcg gacgaagcga catttaactc gtagccatcc agttgaatca
    14281 cgtcgccgaa tgtgattctt gatgagacgg ccggctccct cctcccttcg gtcggggact
    14341 tcgcgggcca agtcacgggc gacggcaacg cgcttgcatc gaatatcaat gccggaatgg
    14401 ggtttcccgc accgtccgtg agcgccgccc aacgccgccg agaccaatcg atcaagttga
    14461 tgtgcgcccg caccagggtg gggcgctgcg catcagggtc gagtggaatg cgatactcgt
    14521 cggcgaagat cgcgcccgcc ggacaatcac tcgtcgccag caagccgcga tgcggatagg
    14581 cgtcgatctt gccgatttcg gcaagatcac gccctttcgc cgaaattgac acgctgtaat
    14641 tgtcctgaat ggggacgagg cactgccaat acagcgtgac cctgagctga ccgtccgagt
    14701 caactctccg aa
//
LOCUS       DBNHXB010000239         3539 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1567610, whole genome shotgun sequence.
ACCESSION   DBNHXB010000239 DBNHXB010000000
VERSION     DBNHXB010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1567610"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="AC162G_09930"
     CDS             complement(<1..676)
                     /locus_tag="AC162G_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076177.1"
                     /translation="MEATNPTNIDVPFPDVGSLHLTIRVGACRLRVAPGESAAWVAGT
                     YHDPTGTLPARIEQEGGNVTITQEVRTGEWWNLLGGNPPRFDLALGKAKAFMLTLEVG
                     ASESSVDLGGLPITRLLIKQGAGKAGFDFSAPNPQAMSLLDLDAGAVGIEMKNLANAN
                     FAEMSVDGGAASYQFDFGGALQREAHVRITTGMSSVEIRVPTSSAARIATESFLGGLE
                     VGDGLTK"
     gene            complement(788..1201)
                     /locus_tag="AC162G_09935"
     CDS             complement(788..1201)
                     /locus_tag="AC162G_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076178.1"
                     /translation="MTTTHFRYVPLGILLIAAFYMFGALVLLISIFINPVGASQAIAL
                     VHGLSPVMGIEILLAVAALALVLAYGLISLSRWGFILTIAYLLYVGGESLVMGGLSFA
                     WTGQPEMQVYFGNLLWSALVTIYLLSVRKRFLGAS"
     gene            complement(1282..2205)
                     /locus_tag="AC162G_09940"
     CDS             complement(1282..2205)
                     /locus_tag="AC162G_09940"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR5076179.1"
                     /translation="MTFITHFAMLKATQRVEHIGQYVTDDGKEKVMKAHIGLLDWIDE
                     VGYLMKAFATRYPFLFAAAVSLTGVLSMLWPFWVPGLSTTTQILLARVSVLVIAVGLL
                     TTLHWWREAGFVKLSSWRVTLPYLPLACLWVLPSLLSLITGGVRVTDLGLILFGCASY
                     LAGAFFEEAIFRGVVLRALLPGGLLRANLLAATLFATVHLAGLAVGADPTAAALQWAY
                     TWLFGFAAVAALAYARNIWPLVVIHFLANYCGYLASGDFFNTAAVTPSQILMAMIIMT
                     PATLYGFWLLRRAGRRQVEPVSAHTSAMQQP"
     gene            complement(2222..2533)
                     /locus_tag="AC162G_09945"
     CDS             complement(2222..2533)
                     /locus_tag="AC162G_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase family protein"
                     /protein_id="HIR5076180.1"
                     /translation="MKILAIEKETPGATKEQFAPHLKAEAARAWELYQAGVLRELYFN
                     RDNHCAVLILECSDVEEARRALDSLPLVKAGRITFDIIPLAPYPGFARLFAGEMPPSA
                     P"
     gene            complement(2670..>3539)
                     /locus_tag="AC162G_09950"
     CDS             complement(2670..>3539)
                     /locus_tag="AC162G_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIR5076181.1"
                     /translation="PAMNNNIGPYHVVDLTPARRVWLNTLELSWSTHCMYGLLEVDVT
                     VARRFIAEHKARTGETLSFTGFLVSCLARAVDEDKAVQAYLKGRKQLVLFDDVNVGLM
                     VEGKIGEKRALMGHVIRGANHKTYREIHQEIRSVQSEPVPPSRGMPTWFRSAMLLPWP
                     LSRLIKALLGMALRRDPTIPVSMAGTVGITSVGMFGGGHSGWGLTPTSQSLGLVVGGT
                     AWKPAVVEGRIEPREILNLTVMFDHDVIDGAPATRFTWRLVELIESGYGLDESSCVAG
                     RLCLNPSARTRPG"
BASE COUNT          736 a         1113 c         1048 g          642 t
ORIGIN      
        1 gcttggtgag gccgtcgcca acctccaggc cgccgaggaa cgattcggtc gcaattctgg
       61 cggcgctcga ggtgggcacg cggatttcga ccgaggacat tccggtagtg atacggacgt
      121 gcgcttcgcg ctggagcgcg ccgccgaagt cgaactggta ggaggccgcg ccgccgtcga
      181 cgctcatctc ggcgaagttg gcattcgcca gattcttcat ctcgatgccg accgcacccg
      241 cgtccaggtc gagcaggctc atcgcttgcg ggttgggcgc ggagaaatcg aagcccgcct
      301 tgcccgcgcc ctgtttgatg agcaggcgcg tgatgggcag gccgcccaga tcgacactgc
      361 tctcgctggc cccgacttcg agcgtcagca taaaggcctt cgctttgccg agcgcgaggt
      421 cgaatcgcgg cggatttccc ccgagcaggt tccaccactc acccgttctg acctcctgag
      481 taatcgtgac gtttccgccc tcctgctcga tcctggctgg caaagtgccg gtcgggtcgt
      541 gataagtgcc agcgacccag gccgcacttt cgccgggcgc gactctgaga cggcaggcgc
      601 ctactctgat tgtcaggtgc aggctgccca cgtcaggaaa tgggacgtcg atgttggttg
      661 gatttgtagc ctccatgatg ctctcctttc aatgatgtga tgcgcgtcaa gcgtgtgcgc
      721 cggtcggagc gatgatgcgc tcgtcacgct catcctcaag gctggctcgt tacacgcagg
      781 gcggtcgtca agatgcgccg agaaagcgct tgcgcacgct gagcagatat atcgtcacga
      841 gcgccgacca gagcaggtta ccgaagtata cctgcatttc cggctggccg gtccacgcga
      901 aactcaggcc gcccatgact aggctttcac cgccgacgta gagcaggtaa gcaatcgtca
      961 agatgaaccc ccagcgcgag agggaaatca gcccatatgc caggacgagc gccagcgctg
     1021 cgacggccag caagatttcg atgcccatga cgggtgatag cccgtgcact aaggcgatgg
     1081 cctggctggc cccaaccgga ttgataaaga tgctgatcag cagcaccagt gctccgaaca
     1141 tatagaacgc cgcaataagc agaatgccca gcgggacgta tctgaagtgg gtggttgtca
     1201 tgacgggtac cctccatgct ccttaagatg ctcaatcatg cggtcacacc ctcggcggtg
     1261 tggctgcggg cacttctttt tctatggttg ctgcatcgca ctcgtgtgcg cagataccgg
     1321 ctcaacctgc cgacgtccgg cacggcgcag cagccagaag ccatacaggg tagccggggt
     1381 catgatgatc atcgccatca gaatctgcga cggcgtcaca gccgcggtgt tgaagaagtc
     1441 gccggaggcc aggtaaccgc agtaattcgc cagaaaatgg atgacgacca gcggccaaat
     1501 gttgcgagcg tacgccagag ctgctaccgc tgcaaaacca aacagccagg tgtaagccca
     1561 ctgtaatgcc gcggcggtag gatcggcgcc gactgccaga ccggcgaggt gcaccgtggc
     1621 gaacagcgtg gccgcgagga ggttggcacg cagtagcccg ccgggcagta gggcacgcag
     1681 cactacccca cggaagatcg cctcctcaaa gaaggcgcct gccagatagc tggcgcaccc
     1741 gaagaggatc aggccaagat cggtcacgcg aacgccgccc gtaatcaggc tcaagagact
     1801 cggcaagacc cacaggcagg ccagcggcag gtaaggcaag gtcacccgcc aactgctgag
     1861 cttgacaaag ccggcttcgc gccaccaatg caatgtggtg agcaggccca cggcaatgac
     1921 aaggacagag acccgggcga gcaagatttg cgtcgtagtc gatagccccg gcacccaaaa
     1981 cggccacaac atgctgagca cgcccgtcag gctgacggca gccgcaaaca gaaaaggata
     2041 acgcgttgca aacgccttca tgagataccc cacctcgtcg atccaatcca gtaacccgat
     2101 gtgcgccttc atgactttct cctttccgtc gtcggtgaca tattgcccga tgtgctcaac
     2161 tcgctgagtt gctttcagca tagcgaagtg ggtgatgaat gtcatggtcg ttcatcactt
     2221 gtcaaggtgc ggatggcggc atctcgccgg cgaataacct cgcaaagcca ggatagggcg
     2281 ccaacggaat gatgtcgaac gtaatccgcc cggctttgac caacggcagc gaatcgagcg
     2341 cccggcgagc ctcttccacg tctgagcatt cgaggatcag gacggcacag tgattgtcgc
     2401 gattgaagta gagctcgcgg agcacgcctg cctgatacag ctcccacgct ctcgcggctt
     2461 ccgctttcag gtggggcgcg aactgctctt tggtcgcacc tggcgtttcc ttctcgattg
     2521 ccagaatctt catgaaatca ccctcgcttc agcccgctct ttgatccctc gcagcatggt
     2581 gctgtgagcc acggtatccc catacccgaa gaccaggcgc atgaccagga agagccacgc
     2641 tggacctcca accgcccgca cgcacaccat cagccgggtc ttgtcctggc cgatgggttc
     2701 aagcacaaac gcccagctac acacgaactc tcgtccagcc catatccgct ctcgatcagc
     2761 tccaccaatc tccacgtaaa gcgggtcgcc ggagccccgt cgatcacatc gtggtcgaac
     2821 atcacggtca ggttgaggat ctcgcgcggc tcgatccgcc cctccactac ggctggcttc
     2881 caggcagtac cccccactac caggccgagc gattgggatg tgggagttag tccccagccg
     2941 ctatggcccc cgccaaacat accaacggag gttatgccga ccgtgcctgc cattgacacg
     3001 ggaatcgtcg gatcacggcg cagggccata ccgagcagag ccttgatcag cctggacagt
     3061 ggccacggaa gcaacatggc agaacggaac caggtgggca tgcctctgct cggcggcacc
     3121 ggttcggact gtaccgagcg gatctcctgg tggatctccc ggtatgtctt gtggttggcg
     3181 ccccggatga catgccccat cagggcgcgc ttttctccaa tcttcccttc gaccatcaac
     3241 ccgacgttta cgtcgtcaaa caggacgagc tgtttacggc ctttcaggta agcctgcacc
     3301 gccttgtcct catccaccgc gcgggccagg caagagacca ggaagccggt gaaggaaagg
     3361 gtctcgccgg tgcgcgcttt gtgctccgcg atgaaccgcc gggcgactgt cacatccact
     3421 tctagcaggc cgtacatgca atgcgtcgac caagacagct cgagcgtgtt cagccacacg
     3481 cggcgcgccg gtgtgagatc gacgacatgg tacgggccaa tgttgttgtt catggcagg
//
LOCUS       DBNHXB010000240         4271 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2406760, whole genome shotgun sequence.
ACCESSION   DBNHXB010000240 DBNHXB010000000
VERSION     DBNHXB010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2406760"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="AC162G_09955"
     CDS             <1..218
                     /locus_tag="AC162G_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076182.1"
                     /translation="LGVSLIWLTRAYDHVVKGEASVEEALAAAQDLFDDYRTCVIARN
                     ALSDPDTQQACQKEVDPTLPDFATGGD"
     gene            459..3326
                     /locus_tag="AC162G_09960"
     CDS             459..3326
                     /locus_tag="AC162G_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076183.1"
                     /translation="MFPTTLPSTLILAARRVWNHRLLMACLLAGLIAAVALLSSIPLY
                     ADAVHHRLMQGELTEEGTYRPPFAFLWRYIGAWHGDIAWDAYAPIDEYLVQQAPAVVG
                     LPLDIQVRHVRTTNLRLFPATETQTFTAREPLLWTSLGFVSGLVDHIQLIEGQFPGDD
                     ASGADVEVLISQTLAEQVGLQVGERYVLFAQGRDAAQIPVRIAGVWRPLNAQDPFWFY
                     QPSSFDEVLLTSEPAFTTGVLPKLEKPIDVAVWYQVFDGSRVRTPDVPRLLDGVKIVE
                     ARATALLNNTTLDASPVQALQTYSQAARLLTVVLTIFGMPVLMLVLYFIGQVAGMVVR
                     RGQGEVALLRSRGATRAQVLFIHLLEFSLVGAIGLIVGLLLGAVLAQLMGQMRSFLDA
                     SPGTSAGEAPLLIVLSWTALWYGLAGVGLAVLASLLPALAASRHTIITFKWEQARMLR
                     RPFWQRYFIDVLLLAVSLYGLFVLRGQQTIALVGQGDDPFSNPLLVLVPALFCFSLAL
                     LFMRVFPALMSGLAWLADRLPGMVVLLTLRQVARSAGQYIGPLLLLIVTLSLATFTAS
                     MAATLDGHLHDQVYYQVGADMNLAELGQSTESPDRPALPGEQPVTLARAEGEPRWLFL
                     PVSEHLRVPGVKAAARAGDYQATSNIGGRQQSGRLLGVDWTDFTQVAFFRLDFAANDS
                     LGGLMNQLALKRSHILVSRSFLARNNLSIGDPLRLTVNVEDVFEPVDFIVAAALDLFP
                     TLYPQDGPFFVANLDYVHESLGETFPYDVWLSTDPSVRGEDIVAGVRDLGLAVVTVSD
                     ARATMTAEQMRPERQGPFGLLSVGFLAAAALTVLGFLVYAVVSFQRRFIELGMLRAVG
                     LSVVQMAAFLAGEQITLVLVGVGIGTGLGVAASNVFIPFLQVGAGKTAQVPPFVVHIA
                     WDKLWIIYAVFGAMFLVAVAVLSLLLARMKVFEAVKLGETV"
     gene            3339..>4271
                     /locus_tag="AC162G_09965"
     CDS             3339..>4271
                     /locus_tag="AC162G_09965"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5076184.1"
                     /translation="MDGEHLRLRCASPQVQVSTNRTRMPFEAIFRLLLLGWLLGIAGC
                     QAPPPQPSTVTITFAAFEFDRKAYESLMAAFHETRPNIEVQFVSLEQALSDDRYDPAS
                     VAAAADTAVIRSTSPGSAASFHDPMPFVEADPAFNASDFWPGLLTACQDGGRVAGVPV
                     GASPSLIFFDGAAFDAAGLPRPAPGWTWSDFQGAARVLTRREGNQVTRYGFVPNGDPF
                     SLIAPVADAMLTTAGDQLNAPTLSGALGWVVDLAREGALPAIEEDPAGLERDEALIAN
                     AQAAMWIDSLINLQWRRSILGPSIGLAPFPISADG"
BASE COUNT          644 a         1363 c         1396 g          868 t
ORIGIN      
        1 ggctgggcgt ctcgctcatc tggctgactc gagcttacga ccacgtggtg aaaggtgaag
       61 ccagcgtcga ggaggccttg gcggcggcgc aagacctgtt cgacgattac cgcacgtgtg
      121 tcatcgcgcg taatgccctg tccgatccag acacgcaaca ggcgtgccaa aaagaggtcg
      181 atcccacttt gcccgacttt gcaacaggtg gtgactaaga ctcattcagg ggaaatagct
      241 gggagctcac atccatgaag ccctttgtac cacacacgaa gggcttcact atcgccccca
      301 tggcccccgg gtgattgcac acatctcgct ccttgcgccg tcctcggcgc gggcagtagc
      361 cgccagggac ggcggctcga gcacttgtgg gtaatcacca gatggccccg ggattgacac
      421 ggtgccccca attgtgacac aatattctcg gtttcgcgat gttccccact acacttccat
      481 cgacgctgat cctggccgcc cggcgcgtgt ggaatcaccg cctgctgatg gcgtgcctgc
      541 tggccgggct gatcgctgct gtcgcgctcc tgtctagcat ccccctatat gccgacgccg
      601 tccatcaccg cctgatgcag ggcgagctga ccgaagaagg cacctaccgc ccacccttcg
      661 ccttcttgtg gcgatacatc ggcgcctggc acggcgacat cgcctgggat gcctacgcgc
      721 ccatcgacga gtatctcgtt caacaggctc cggctgttgt cgggctgccg ctggacatac
      781 aggttcgcca cgtccgcacc accaacctgc gcctcttccc cgcaaccgaa acgcagacgt
      841 ttacggcgcg tgagccgctg ctgtggacca gcctgggatt cgtcagcggc ctggtcgatc
      901 acattcaact gatcgagggc cagttccccg gcgatgatgc gtccggcgcc gacgtcgagg
      961 tgctcatcag ccagacgttg gccgagcaag tgggtctgca agtcggcgaa cgctacgtcc
     1021 tgttcgctca ggggcgcgat gccgctcaga taccggtgcg cattgccggt gtgtggcgac
     1081 ctctcaacgc gcaagacccc ttctggttct accagccctc gtccttcgat gaagtgctgc
     1141 tcacgtcgga gccggctttt acgaccggcg tcttgcccaa actggagaag ccgatcgacg
     1201 tcgccgtatg gtatcaggtc ttcgacggca gccgcgtgcg aacgccggac gtgccgcgcc
     1261 tgctcgacgg tgtcaagatt gtcgaggcgc gcgccaccgc cttgttgaac aacaccacac
     1321 tcgatgcctc gccggttcag gcgttgcaga cctacagcca ggcggcgcgg ttactgacgg
     1381 tcgtgctgac gatcttcgga atgccggtgc tcatgctggt gttgtacttc atcgggcagg
     1441 ttgccggcat ggtcgtgcgg cgcggccagg gcgaggtcgc ccttctgcgc agccggggcg
     1501 cgacgcgcgc gcaggtgctc ttcatccacc tgctcgaatt ctcgctcgtc ggcgccatcg
     1561 gcctgatcgt tgggctgttg ctcggcgccg tactggcgca gttgatgggc cagatgcgtt
     1621 cgttcctcga cgcttcgccc ggcacaagcg caggcgaggc gccgctgttg atcgtgctct
     1681 cgtggacggc gctgtggtac ggcctggcgg gagtggggtt ggccgtgctg gcttcattgc
     1741 ttccggcgct ggcggcctcg cggcacacga tcatcacttt caagtgggag caggcgcgca
     1801 tgctgcgccg ccccttctgg cagcgctact tcatcgacgt gttgttgctg gcggtcagcc
     1861 tgtacgggct gttcgtgctg cgcggccagc agacgatcgc gttggtcggg caaggcgacg
     1921 atcctttctc caatccgctc ctggtgttgg ttcccgcact gttctgtttc tcactggcgc
     1981 tgctcttcat gcgcgtcttt cccgcgttga tgtccgggct ggcctggctg gccgatcgac
     2041 tgccgggcat ggtggtgttg ttgacgctgc ggcaggtcgc tcgatccgcc gggcagtata
     2101 tcgggccgct gttgttgctg atcgtgacgc tgagcctggc gacgttcacc gcgtcgatgg
     2161 cggccacgct cgatgggcat ttgcacgatc aggtttacta ccaggtcggc gcggacatga
     2221 acctggccga gttgggccag agcaccgagt cgcccgatcg gccggcgctg cccggcgagc
     2281 aacctgtaac gttggcgcga gccgaaggcg agccgcgctg gctgttcctg cccgtcagcg
     2341 agcatttgcg cgtgccgggc gtcaaagcgg ctgcgcgcgc gggcgactac caggccacgt
     2401 cgaacatcgg cgggcggcag caatcgggcc gcttgctcgg cgtcgattgg acggacttca
     2461 cccaggtcgc tttcttccgt ctcgacttcg ccgcgaacga ttcactcggc gggttgatga
     2521 atcaactggc gctcaagcgc agccacatcc tggtcagccg cagtttcctg gcgcgcaaca
     2581 acctgtcgat cggcgatccg ctgcgcctga cggtgaacgt cgaggatgtc ttcgagccgg
     2641 tcgatttcat cgtggcggct gcgctcgatc tgttcccgac actctatccg caagatgggc
     2701 cgttcttcgt cgccaacctc gactatgttc acgagtcgct gggcgagacg ttcccctacg
     2761 acgtgtggtt gtccaccgac ccgtccgtgc gcggcgagga catcgtcgcc ggtgtgcgcg
     2821 acctgggatt ggccgtcgtc accgtgtcgg atgcacgcgc gacgatgacg gccgagcaga
     2881 tgcggccgga gcgtcagggg ccgttcgggt tgttgtcggt cggatttctg gcagccgccg
     2941 cgttgacggt gttaggcttc ctggtgtacg ccgtcgtctc cttccaacgc cgatttatcg
     3001 aactgggcat gctgcgcgcg gtcggcctct cagtcgtgca gatggcggcg tttctggctg
     3061 gcgaacagat cacgctcgtc ctggttggtg tggggatcgg caccggcctc ggcgtggcag
     3121 ccagcaacgt gttcatcccg ttcctgcaag tcggtgcggg caagaccgcg caggtgccgc
     3181 ccttcgtcgt gcacatcgcg tgggacaaac tgtggatcat ctacgcggtc ttcggcgcga
     3241 tgttcctggt ggcggttgcc gtgctgagcc tcctgctcgc gcgcatgaag gtcttcgagg
     3301 ccgtgaagct aggcgagacg gtataaggga gtcagcgaat ggacggcgaa cacctgcgct
     3361 tgcgctgcgc gtcaccgcag gtgcaagtgt caacgaatcg aacacgcatg ccattcgagg
     3421 ccatcttccg gctgctgctg ctgggatggc tcctgggtat agcgggatgc caggcacccc
     3481 cacctcagcc gtcgacggtg acgatcacct tcgccgcatt cgagttcgac cgcaaagcct
     3541 acgagtcgtt gatggccgcg ttccacgaaa cacgcccgaa cattgaggtg caattcgtgt
     3601 cgctggagca ggcgctgagc gacgatcgct atgatccggc atcggtggcg gcagcggcgg
     3661 acaccgccgt gatccgctcg acatccccag ggtcggcagc ttccttccac gatccgatgc
     3721 cgttcgtcga agccgatccg gcgtttaatg cttcggactt ctggcctggc ttgctgacgg
     3781 cctgtcaaga cggggggcgc gtcgcaggtg tgccggtggg cgcaagcccc agcctgatct
     3841 tcttcgatgg cgcggccttc gacgccgccg gtctgccgcg cccggcgcct ggctggacgt
     3901 ggagtgactt tcaaggcgcg gcccgcgtgc tgacacggcg agaaggcaac caggtgactc
     3961 gctacggctt cgtgccgaac ggcgatccat tttcgctgat cgcgccggtg gcggatgcga
     4021 tgctgaccac tgccggcgac cagttgaacg cgcccactct gtccggcgcc ttgggctggg
     4081 tcgtcgattt ggcgcgcgaa ggcgcactgc ccgctatcga ggaggacccg gctggcctgg
     4141 agcgcgacga ggctctgatt gcgaacgcgc aggcggcgat gtggatcgac agcctgatca
     4201 acttgcagtg gcggcgcagc atcctggggc cgtcgattgg gcttgcgccg tttccgattt
     4261 cggcggatgg a
//
LOCUS       DBNHXB010000241         5108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1400792, whole genome shotgun sequence.
ACCESSION   DBNHXB010000241 DBNHXB010000000
VERSION     DBNHXB010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1400792"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..681
                     /locus_tag="AC162G_09970"
     CDS             214..681
                     /locus_tag="AC162G_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076185.1"
                     /translation="MSTNRLLYVLVAVALVVTAALVIRAGVATSEVVASGSNLRAWNA
                     VGERYTAMAEALKPVSAYTARWTGLADSFKDANRAQEAYAARYAGLAQAQRSINAYGA
                     RWSGLADSLTGASAIDAYSARLSGLAELFTNKSRAIEAYGARWTGLAQAFANK"
     gene            complement(761..2476)
                     /locus_tag="AC162G_09975"
     CDS             complement(761..2476)
                     /locus_tag="AC162G_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076186.1"
                     /translation="MDETFLVLGGGGMVGTQVVRQIARHLAPRRIIVASLYQQEVRET
                     ISRLEREFPSVHFVGFWGDVFLRAEWNSHDRRRQTTRARMLDSAERRAGMYDDLLGDP
                     DAAYARSQLVRLILEHRPDVVVDSINTATAISYQDVHAASIVAKKRFDALMSDVEQGD
                     IGRAVGAGVSARRALETLLISQSVPQLVRHVLLIHRAMSEAGTRLYLKVGTTGTGGMG
                     LNIPYTHSEDKPSAKLMTKTAIAFAHTGLMFLMARTPGVPVVKEVKPGAMIGYADITR
                     RTIRERGKPVTVFASHTEPLGDTLSLRQPESSYERCGPLTMVVVDTGENGLFTKGEFE
                     TITHMRQMEFITPEEIALQVVLEIKGSNTGYDVIAAIDSAVMNPTYRAGYLRYFALEE
                     IARLERETQSHSVALGQLGPPELGKLLWESHLLKLRFGTLSAVLEQTIDALAAEVFDL
                     LQSDTKLRDTITAVGLPILTPDGHALIRGPFIRIPEMPGTNTVALTPERVEQWAAKGW
                     VDLRPRNFERWQERFRRMRREAQRLRGRGSAAITREAYLSDDISIGAVVGWIFNNEAG
                     AYRIK"
     gene            complement(2641..3030)
                     /gene="rplL"
                     /locus_tag="AC162G_09980"
     CDS             complement(2641..3030)
                     /gene="rplL"
                     /locus_tag="AC162G_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR5076187.1"
                     /translation="MSEKLEKLVAELDSLSLVEAAELSKMLQDKWGVSAAAPVMMGAM
                     SAAGAAPAAAAPVEEKTEFDVIIKDAGPKKIEVIKTVRQLTSLGLKEAKDLVDTAPQP
                     VLTAVAKEAANDAKSKLEAAGAVVEVK"
     gene            complement(3189..3719)
                     /gene="rplJ"
                     /locus_tag="AC162G_09985"
     CDS             complement(3189..3719)
                     /gene="rplJ"
                     /locus_tag="AC162G_09985"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR5076188.1"
                     /translation="MPLTKERKSELVAEYAEWLRRSSAVVVAEFPGVGAKDLYNLRVK
                     LRESNSQLHVVKLTLFVRALAEAGLPIPEDMLTGPLVMAFAHEDAPAAAKAMTEFASS
                     IESFQVKGGLLGDRIIDAAGVKMLADLPPRPVVLAGLVGTLQGPMSQLVSVLQAPLRE
                     IAQVLKARSEQAPAAT"
     gene            complement(3990..4712)
                     /gene="rplA"
                     /locus_tag="AC162G_09990"
     CDS             complement(3990..4712)
                     /gene="rplA"
                     /locus_tag="AC162G_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR5076189.1"
                     /translation="MKRHGKKYLAAAQKVDDQKLYTPSEAMALVKETSITKFDSTVEV
                     HMRLGVDPRHADQQVRGVVLMPAGVGKTVRVTVFAQGEAARIAEDAGADTVIGDDEGL
                     KKIQDGWTDWDISLAMPDMMGKVGRLGKILGVRGLMPNPKAGTIAAKADDLPRMIKEA
                     KAGRVEFRIDKTANIHVIIGKASFGAEQLMTNFAALMDALQKAKPSAAKGTYLKRVVV
                     TSTMGPGIKVDPNLALSLQASV"
     gene            complement(4809..>5108)
                     /locus_tag="AC162G_09995"
     CDS             complement(4809..>5108)
                     /locus_tag="AC162G_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL11 family ribosomal protein"
                     /protein_id="HIR5076190.1"
                     /translation="NARTASMAGQIIPAELTIFSDSSFTFVLRTPPTADMLRRAAGVE
                     KGSSAPNKDKVATISRKQLREIAEAKLKDLNTADVEMAMQQVAGTARSMGIDIKD"
BASE COUNT          864 a         1711 c         1549 g          984 t
ORIGIN      
        1 caattcaagt atatcaccaa acgagcctga aatgaatacc gatttcacca agacttccga
       61 agtcttgaag acttcggaag tctggcatgc aaaacacgct gaataacgct gacgaacaca
      121 ctgtcgaacg ccggcctgat acagtggcgg caacaaaaag ggccgggctg cgagaaacgc
      181 gaaatctaac tcggccaagt cagggaggtc gaaatgtcta ccaatcgttt gctgtatgtg
      241 ctggtcgcag tcgctctggt ggtgacggct gccctcgtca tccgtgcagg ggttgccacc
      301 tcggaagtcg tggccagtgg gtcaaacctg cgggcctgga acgccgtggg cgagcgctac
      361 accgccatgg ccgaagccct gaagcccgtg agcgcttaca ccgcgcgctg gacgggtctg
      421 gccgattcgt tcaaggatgc gaaccgggcg caggaagcct acgcggcgcg ctacgcaggc
      481 ctggctcagg cgcagcgcag catcaacgcc tatggcgcgc gctggtccgg tctggcggac
      541 tcgctcaccg gcgcaagcgc gatcgacgcc tactccgcgc gcctgtcagg gctggccgag
      601 ttgttcacga acaagtctcg ggctattgag gcctacggcg cgcgctggac gggcttggcc
      661 caggcgttcg caaacaagta agctgatggg cgccagctac aacatcccta ttcgcagacg
      721 acctgcgccg ggcggagcct ccggcgcagg tcttttcatc tcacttgatc cgatacgcgc
      781 ccgcttcgtt gttgaaaatc caccccacga ccgcgccgat cgagatgtcg tccgaaagat
      841 aagcctcgcg cgtgatagcc gccgagcccc gcccgcgcag ccgctgcgcc tcgcgccgca
      901 tacgacggaa acgttcctgc cagcgctcga agttgcgcgg ccgcagatcg acccaaccct
      961 tggcggccca ttgctcgacg cgctccggcg tcagcgccac agtgtttgtc cctggcattt
     1021 ccgggatgcg gatgaagggg ccgcgtatca gggcgtggcc gtccggcgtg aggatgggca
     1081 agccgacggc ggtgatagtg tcgcgcagtt tcgtgtcgga ttgcagcagg tcgaacacct
     1141 cggcagccag cgcgtcgatc gtctgctcga gcaccgcgct gagtgtgccg aatctcagct
     1201 tgaggagatg agattcccac agcagcttgc ccagctcggg cggtccgagc tggccgagcg
     1261 ccaccgagtg cgactgcgtc tctcgctcca ggcgcgcgat ctcttcgagc gcgaagtagc
     1321 gcaggtagcc ggcgcggtag gtcgggttca tcaccgcgct gtcgatcgcg gcgatcacgt
     1381 cgtagccggt gttgctgcct ttgatttcca ggaccacctg cagcgcgatc tcctccggcg
     1441 tgatgaattc catctgccgc atatgggtga tcgtctcgaa ctcgcccttg gtgaacaggc
     1501 cgttctcacc cgtgtcgacc acgaccatgg tcagcgggcc gcaacgctcg tagctcgact
     1561 cgggctggcg cagcgagagc gtgtcgccca gcggctccgt gtgactggcg aagacggtca
     1621 ccggcttgcc gcgctcgcgg atcgtccggc gcgtgatgtc ggcatagccg atcatcgcgc
     1681 cgggcttgac ttctttgacg accgggacgc cgggcgtgcg cgccatgagg aacatcaaac
     1741 cggtgtgggc gaaggcgata gccgtcttag tcatcagctt ggccgagggt ttgtcttccg
     1801 agtgcgtgta cggaatgttc aagcccatgc cgcccgtgcc ggtcgtcccc accttgagat
     1861 acaggcgtgt cccggcctcg gacatggccc ggtggatcaa cagcacgtgc cgcaccagtt
     1921 gcggcaccga ctgcgagatg agcagcgtct ccagcgcacg ccgggcagaa acgcctgccc
     1981 cgaccgcccg gcctatgtcg ccttgctcga cgtcagacat tagcgcgtca aaccgcttct
     2041 tcgcgacaat cgaagcggcg tgcacgtcct gataactgat cgccgtcgcc gtgttgatgc
     2101 tgtccacgac cacgtcgggc ctgtgctcca ggatcagccg cacgagctgc gagcgggcgt
     2161 acgccgcgtc tgggtcgcct aacaaatcgt cgtacatccc tgcgcggcgc tccgccgaat
     2221 cgagcatccg cgcgcgcgtc gtctggcggc gacgatcgtg cgagttccac tcggcgcgca
     2281 ggaacacgtc gccccagaag ccgacgaagt gcacggaagg aaactcacgc tcgaggcggg
     2341 aaatcgtctc acgcacttct tgctggtaca acgaggcgac gatgatgcgt cgcggcgcaa
     2401 gatggcgcgc gatttgtcgc accacctgcg tgccgaccat ccctccacca cccaacacca
     2461 gaaatgtttc gtccatgggc gactctttct ttttcggatc ctccgtcgca gctgcgaacg
     2521 gcatggggat accgttgcta cgtaagcata cacgcggctg aaggcggggt caagcaaaac
     2581 ctcccgcagg cgcgcagcct gcgggaggtt cggacattgc atgcacggac gagacggcgc
     2641 ttacttgact tcgacgaccg cgcccgccgc ttcgagcttg gacttggcgt cgttagcggc
     2701 ttccttcgcc acggcggtca gcaccggctg cggagccgta tccaccaagt ccttggcttc
     2761 cttcaggccg aggctggtca actggcggac ggtcttgatg acctcgatct tcttcgggcc
     2821 agcgtccttg atgatcacgt cgaactcggt cttctcttcg accggggccg cggcagcggg
     2881 cgccgcgccc gccgccgaca tcgcgcccat catcaccggg gcggcggcgc tcacacccca
     2941 cttatcctgc agcattttcg acagctcggc cgcttccacc aggctcagcg agtctagctc
     3001 ggcaaccagc ttctcaagtt tctcagacat atcgaatccc tcttttcttt gatttacatt
     3061 tccttcgttt ccttcagttc cgacgagcca acgaagctca gggaaccccc gcagacagcc
     3121 ggcgagggca ggcccccgca cacagccggc gagggcgggc caaaggaact cccgtcaggt
     3181 ccgggtgttc aggtggcggc tggtgcctgc tctgaccgcg ccttcagcac ctgcgcgatt
     3241 tcgcgcagcg gcgcttgcag cacgctgacg agctggctca tcgggccttg cagcgtgccg
     3301 actaacccgg ccagcacgac cggcctcggc ggcaggtcgg cgagcatctt cacgcccgcc
     3361 gcgtcgatga tccggtcgcc gagcagcccg cctttcacct ggaacgactc gatgctgctc
     3421 gcgaactccg tcatcgcctt cgccgcagct ggcgcatctt cgtgcgcgaa ggccatgacc
     3481 agcggcccgg tcagcatatc ttccgggatc ggcaggccgg cctcggccag cgcgcgcaca
     3541 aacagcgtaa gcttcacgac gtgcaactgg ctgttagact cgcgcagctt cacgcgcagg
     3601 ttatacaggt ccttcgcgcc gacgcccggg aactcggcga cgaccaccgc gctgctccgg
     3661 cgcagccact cggcgtattc ggccaccagc tccgacttgc gctctttcgt taacggcact
     3721 cttcttcacc tcctttcgcg ttcgctgtcc agcacgacgc ggatttcgca cggattacac
     3781 ggacattagt gtggacaacg aaaagtcttt gccgcagccg tggggtcgcc aagcgactca
     3841 cacgaggcaa agactgtgac gcgagcgggc acattgcccg cacaatgcgt gccacgagaa
     3901 tcttgcctcg gcaggctacc atcattaagc cgctcacctt cgcggcgcct gctgtctccg
     3961 gctgactgcg gcgctattcg gttgtcgaac tacaccgacg cctggagcga cagcgccagg
     4021 ttcgggtcga ccttgatgcc cggccccatc gtcgacgtga cgacgacgcg cttcaggtac
     4081 gtgcccttcg ccgcggacgg cttggccttc tgcagggcgt ccatcagtgc ggcgaagttc
     4141 gtcatcaact gctcggcgcc gaacgaggct ttgccgatga tgacgtggat gttcgccgtc
     4201 ttatcgatgc gaaactcgac gcggcctgcc ttggcttctt tgatcatgcg cggcaggtcg
     4261 tccgccttcg ccgcgatcgt gccggccttg gggttgggca tcaggccgcg tacgccgagg
     4321 atcttgccca gacgcccgac cttgcccatc atgtcgggca tcgccaacga gatgtcccag
     4381 tccgtccagc catcctggat cttcttcagg ccctcgtcgt cgccgatgac ggtatcggcg
     4441 ccggcgtctt cggcgatcct ggctgcctcg ccctgcgcga agacggtcac ccgcaccgtc
     4501 ttgcccacac cggccggcat gagcacgacg ccgcgcacct gctggtcggc gtgacgcgga
     4561 tcgacgccca ggcgcatgtg gacttccacc gtgctatcga acttcgtaat cgacgtctcc
     4621 ttcacgagcg ccatcgcctc ggagggcgtg tacagcttct gatcgtcgac tttttgtgct
     4681 gctgccagat atttcttgcc gtgacgcttc actgtcattg ctccttgtgg tgcgatcggg
     4741 ccgacgccag tccggccctc ccacgctctc actcacaaac caggtttctt ggagaagtgt
     4801 ggtttgtgct agtctttgat gtcgatcccc atgctgcgcg ccgtgcctgc gacctgctgc
     4861 atcgccatct cgacgtccgc cgtgttcaag tccttcagct tggcttcggc aatttcacgc
     4921 agttgcttgc gactgatcgt cgccactttg tccttgtttg gcgcgctcga tcccttctcg
     4981 acacctgccg cgcggcgaag catatcggcc gtcggcggcg tgcgcagcac gaaggtgaac
     5041 gaggagtcgc taaagatcgt cagctcggcc gggatgatct ggccggccat acttgccgtg
     5101 cgcgcgtt
//
LOCUS       DBNHXB010000242         2870 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2910622, whole genome shotgun sequence.
ACCESSION   DBNHXB010000242 DBNHXB010000000
VERSION     DBNHXB010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2910622"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="AC162G_10000"
     CDS             <1..1261
                     /locus_tag="AC162G_10000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5076191.1"
                     /translation="PGQQAHSPDSATPRPELANSPADHADVIGSSIAGLMAARVLSEH
                     FACVTVVERDWLPDGPAYRRGVPQARHAHTLLPQGQMILEQLFPGLVDELLAGGAIAI
                     DAARDIAFFEAGAWHTPRARDVKVSIACSRPLLENVIYRRVASRANVHIMPGYETLGL
                     QAGEWRERVTGIRIRRRQAGAAEAVLAAELVVDASGRQSQAPRWLYSLGYTPPEEWAI
                     NSFTGYATRSYRRPAGFTGGWKTLYVRPTPANGTRGGIILPMEGDRWHVTLIGVAGDY
                     PPTNEQGFLAFARSLPTPRLYEAIESAEPLTKPSGFRRTENRVRRYDKLPRYLEGFVV
                     CGDAVYALNPIYAQGMTAAATGSLVLDECVQEQRRRQGAGGITGLAETYHKQLSKAVA
                     GLWRTATRKDWDWPITEVDDSVYQPHQ"
     gene            1626..2507
                     /locus_tag="AC162G_10005"
     CDS             1626..2507
                     /locus_tag="AC162G_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5076192.1"
                     /translation="MIQKIILAVDGSEHSMAATLLIRDLPFPRSSSVTALAVLTPLQT
                     PGRAALMAALGKTQSILESGNFDVKTGILHGHPAEELAKFADEHRPDLLVVGAKGLRA
                     TLGILLGGVAQQLVEYAHWPVLVVRAPYAGLRRVLLVTDGSTHSREAVDYLKKFPLRA
                     NTTVEVMHVLPPMPQPYVSTPAWPAGTNMPPLVPEALEEEVTARQAEAEELEGKAIVD
                     SAVRTLRSAGIAATGVLVRGDAATEIIEHVKAHVTDLIVAGSRGYGAVKGWLMGSVSR
                     KLVHYAGCSVLIVRSEL"
     gene            2572..>2870
                     /locus_tag="AC162G_10010"
     CDS             2572..>2870
                     /locus_tag="AC162G_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076193.1"
                     /translation="MRLRNVATIAVKDLVDALKNFRLLAIVLMPIALSIVFGLLYRDA
                     PSGSTIVVYDPGESQIVTSIGATDGWSLWEVDSEADVERAMTEQEALAGLVLPAD"
BASE COUNT          519 a          898 c          950 g          503 t
ORIGIN      
        1 gccaggacag caagcacact ctcctgactc agccacgccc cggcccgagc tggccaactc
       61 gcccgccgat catgccgacg tcatcggcag cagcatcgca ggcctgatgg cggctcgggt
      121 cctttccgag catttcgcct gcgtcacggt cgtcgaacgt gactggctac ccgatgggcc
      181 ggcctatcgc agaggtgttc cccaggcgcg ccacgcccac acactgctgc cacagggtca
      241 gatgatcctg gagcagctct ttcctggact ggtcgatgag ctgctggccg gtggcgctat
      301 cgctatcgat gccgctcgcg acatcgcctt cttcgaggcc ggcgcctggc acacgccacg
      361 ggcaagggat gtcaaagtgt cgatcgcctg cagccggccg ctgctggaaa acgtcattta
      421 ccgtcgggta gcatcgcgcg ccaacgtcca catcatgccg ggatacgaaa ccctcggtct
      481 ccaggccggc gagtggcgcg agcgcgtgac cggtatccgc attcgtcgtc gccaggctgg
      541 cgcggccgag gcggtgctgg cagcggagct ggtcgtcgac gccagtggcc ggcagtcgca
      601 ggccccacgg tggctgtata gcctcggtta cacaccgccg gaagagtggg cgatcaactc
      661 gttcacgggg tacgccacac gcagttaccg ccgtcccgcc gggttcaccg gcggctggaa
      721 gacactgtac gttcgaccga cgccggccaa cggcacgcgc ggcggcatta ttctccctat
      781 ggagggcgac cgctggcacg tgacgctcat cggcgtggcg ggtgactatc cgccgacgaa
      841 cgaacaggga ttcctggcct tcgcccgcag cctgcccacg ccgaggctgt acgaggcgat
      901 tgagagcgcc gagcctctga cgaagccgtc cgggttccgc cgcaccgaaa atcgcgtgcg
      961 ccgctacgac aagctgcctc gttacctgga gggattcgtc gtgtgcggcg atgcggtgta
     1021 cgccctgaac ccgatatatg cccaggggat gacggctgcg gcgacaggca gtctggtatt
     1081 ggacgagtgc gtgcaggaac agcgccgacg gcagggcgcc ggcggcatca ccggcctggc
     1141 cgagacctac cataagcagt tgagcaaagc ggtggccgga ctgtggcgca cagcgacgag
     1201 gaaggattgg gactggccca tcacggaagt cgacgacagt gtctaccagc cacatcagtg
     1261 aacggcaatg ccgccggaac tgatcgcgca ggcgcggcgg ccgtcgacag ccgtgtgtgc
     1321 gcgcaaaaca tgccagcggc gcgtatagaa acgcgcccta cggttgacaa cctttgcgtg
     1381 cgcactccgt ttcgccacca cttgatttct tggcggaaat atcttatact gacagtgaaa
     1441 ttagtgtaaa caagggaatg cctcaggccg tattgacaca ctggggccac agccaaggcg
     1501 gagggggggc cttggtgggg agcgaataga atgcctgccc accagtcgat gaggcaggca
     1561 ttttgcttat tcttccgagt gcgtgcctgt atcgcctgtg ggtgaagacg agtaggagat
     1621 ttgccatgat tcagaaaatc atcctcgccg tcgatggctc cgagcactcc atggcggcga
     1681 ctctactgat acgtgatttg ccgtttcccc ggagcagtag cgtgaccgct ttggccgtcc
     1741 tgacgccgct ccagacgccc ggccgggctg cattgatggc cgcgttgggc aagacccaat
     1801 cgatcctgga atccggcaac ttcgacgtga agaccgggat actgcacggc catccggctg
     1861 aagagctggc gaagttcgcc gacgagcacc ggcccgactt gctcgtcgtc ggcgcgaagg
     1921 gcctgcgcgc gacactgggc attctgctcg gcggcgtggc gcagcaactg gtcgaatacg
     1981 cgcactggcc tgtcctggtc gtgcgagcgc cgtacgccgg gctgcggcgc gttctgctcg
     2041 tcaccgacgg ttctacccac agccgggaag ctgtggacta tctgaagaaa ttcccgcttc
     2101 gcgctaacac aaccgtagag gtcatgcacg tcctgccgcc aatgccacag ccgtatgtct
     2161 caacccccgc ctggcccgca ggcacgaaca tgccgcccct ggtgccggag gcgttagagg
     2221 aagaggtcac cgccaggcag gctgaggcgg aggagcttga ggggaaagcg atcgtcgaca
     2281 gcgcagtgag aaccctgcgc agcgccggca tcgccgcgac cggcgtcctc gtgcgcggcg
     2341 atgcggcgac cgaaatcatc gagcatgtca aggcgcacgt gaccgatctg atcgtcgccg
     2401 gctcgcgcgg ttacggcgcg gtcaagggct ggctgatggg cagcgtctcg cgcaagctgg
     2461 tgcattatgc cggatgctct gtcttgattg tacggagcga gctttaggtc gacacctgag
     2521 ttgccgccgc tcgatcgcgc agggggtcgg gcgcgagcag tgggagatgt catgcgtctc
     2581 agaaacgtcg caacgattgc cgtcaaggac ctggtcgacg cgctgaagaa ttttcgactg
     2641 ctggccatcg tcctgatgcc gatcgcgttg tcgatcgtgt tcgggcttct gtaccgggac
     2701 gcgccgtccg gctcgacgat cgtcgtctac gatccgggcg agtcgcagat cgtcacgtcg
     2761 attggagcga ccgacggctg gagcttgtgg gaagtcgatt ccgaagcgga cgtcgagcgc
     2821 gcgatgacgg agcaagaggc gctggctggc ctcgtgctgc cggccgactt
//
LOCUS       DBNHXB010000243         3559 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_59239, whole genome shotgun sequence.
ACCESSION   DBNHXB010000243 DBNHXB010000000
VERSION     DBNHXB010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_59239"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /gene="hpaE"
                     /locus_tag="AC162G_10015"
     CDS             <1..1430
                     /gene="hpaE"
                     /locus_tag="AC162G_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HIR5076194.1"
                     /translation="AEGTFETLNPATNRPIGVVADGQPEDVDRAVKAARKAFDDGPWP
                     KMNAEARSKKLRKIADLIEKYADEIAEREIVDCGVPIRQIRGGAIPRAAYNFNYFADM
                     TYRVTGESYPVGDQFINYSIRRPVGVAGLITPWNVPFMLETWKVAPCLAFGNTCILKP
                     AEWSPLSADMLARIVQEADLPPGVFNIVHGFGETAGAPIVAHPGVQVISFTGETTTGQ
                     IIMRNGASTLKRYSMELGGKSPTIVFEDADMERALDGTIFQVYSLNGERCTAGSRLVL
                     QESIYDTFVEKLAARVAKIKVGPPEDPATEVGPLIHPEHWEKVNGYMDIARTEGATIR
                     VGGVRPKGFPEGSYFSPTLIVNVRNDMRIAQEEIFGPVLVVIPFKDEAEAIRIANDVR
                     YGLAGYVWTQDIGRGHRVAQAIESGMVWINSHNVRDLRTPFGGSKHSGIGREGDHYSF
                     ELYTELKAIHVALGTHHIPRMGATD"
     gene            1542..2999
                     /gene="hpaB"
                     /locus_tag="AC162G_10020"
     CDS             1542..2999
                     /gene="hpaB"
                     /locus_tag="AC162G_10020"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HIR5076195.1"
                     /translation="MGARRGKDVIQKLKEQPAELWHRGKRVADVTAEPGLANGVASLA
                     ELYDLQWEDPGTMLFDSPTTGDKVGRSYLIPRTKEELMSVGTMMMRWTRHTMGMMGRA
                     PDYINRAISAYASGADFLAKQDARFGENIRRYHEYARENDLCLTHTLINPQANRAVGA
                     AQQADPFLAARVKTETDAGIVVKGARMLATLPISDEIMVFPSTLLKSGEEDAPYAFGF
                     AIPNSTPGLKFICRESVDYGRSKYDHPLGSRFEEIDATVIFDDVLVPWERVFLYRDVA
                     ACNRAYSATGAVVHMSHQVVCKNVAKTEFVLGLVSLMVDAIGVEQFQHIHEKVAEVWC
                     ILEAMKAFKRVAEVDAAPNEYGVMTPAWDPLDAARNMYPRLYPRLIEIVQQVGASGLV
                     SMPTEADVNGPLAEDIRKYYQAARMGALERIPLFRLAWDTAVSAFGSRQVLYERFFFG
                     DPVRMAGVLFQGHDRKPYMDMVREFLRRSARDGEE"
     gene            3020..>3559
                     /locus_tag="AC162G_10025"
     CDS             3020..>3559
                     /locus_tag="AC162G_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR5076196.1"
                     /translation="MDKRGFDFIFRAMSHPSNSSTIDPRAYRNTIGAFATGVTVVAAG
                     HGNHLHAMTANAVTSLSLDPPLLIFCVSKKARIVEVLEAAHGFSVNILRDSQQALSTY
                     FAGGWKEPAPPPFRFVPWEGGPRLEGCAASIGCTLHERIEGGDHWIVVGHVVALHQGE
                     EPQRPLIFYGGKYRHLDAAG"
BASE COUNT          684 a         1090 c         1188 g          597 t
ORIGIN      
        1 tcgccgaagg gacgtttgag acgctcaacc cggcgacgaa ccggccgatc ggcgtcgtgg
       61 ccgacgggca gccggaagac gtggatcgcg cggtgaaggc ggcgcgcaag gcgttcgacg
      121 atggcccctg gccgaagatg aacgccgagg cgcgctcgaa gaagctgcgc aagatcgccg
      181 atctgatcga gaaatacgcc gatgagatcg cggagcgcga gatcgtcgac tgcggcgtgc
      241 cgatcaggca gatcagaggc ggcgctatcc cgcgcgcggc gtacaacttc aactacttcg
      301 ctgacatgac ttaccgcgtg accggcgaga gttacccggt gggcgatcag ttcatcaact
      361 acagcatccg tcgcccggtc ggcgtggcgg gcctgatcac gccgtggaac gtcccgttca
      421 tgctggagac gtggaaagtc gcgccgtgcc tggccttcgg caacacctgc attctcaagc
      481 ccgccgaatg gtcgccgctc tcggccgaca tgctcgcgcg catcgtccag gaggccgacc
      541 tgccgccggg cgtgttcaac atcgtgcacg gcttcggcga aacggcgggc gcgccgatcg
      601 tcgcgcatcc gggcgtacaa gtcatctcgt tcaccggcga gacgacgacc ggccagatca
      661 tcatgcgcaa cggcgcgtcg acgctcaagc gctactcgat ggagctgggc ggcaagtcgc
      721 cgacgatcgt cttcgaggac gccgacatgg agcgcgcgct cgacggcacg atcttccaag
      781 tctactcgct caacggcgag cgctgcacgg ctggctcgcg cctggtcctg caggaatcga
      841 tttacgatac atttgtcgag aaactcgccg cgcgggtggc gaagatcaag gtcggcccgc
      901 cagaagatcc ggcgacggag gtcgggccac tgattcaccc tgagcattgg gagaaggtga
      961 acggttacat ggacatcgct cgcactgaag gggcgacgat ccgggtcggc ggcgtgcggc
     1021 caaagggttt cccggagggc agttatttca gcccgacgct gatcgtcaac gtccgaaacg
     1081 acatgcgcat cgcacaggaa gagatcttcg ggccggtgct ggtggtcatt ccgttcaagg
     1141 acgaggccga ggcgatccgt atcgccaacg acgtgagata cgggttggcc ggatacgtgt
     1201 ggacgcagga catcggccgc gggcatcgcg tggcgcaggc catcgagtcg ggcatggtgt
     1261 ggatcaactc gcacaacgtg cgcgacctgc gcacaccgtt cggcggctcg aagcactccg
     1321 gcatcgggcg cgaaggcgac cactacagct tcgagctgta caccgagctt aaggcaattc
     1381 acgtcgcatt aggcacgcat cacattccga ggatgggggc aacggattaa cagcggataa
     1441 gcggatgcag taccgcgcag accgtatccg tttatccgat gcctattcgt tgcttcgctg
     1501 ttcagcattc cgataagccg tttctaaatt ctggagggaa gatgggcgca cgacggggta
     1561 aagatgtcat ccagaagctg aaggagcaac cggccgaact ctggcatcgc ggcaagcgcg
     1621 tcgcggacgt gacggccgag ccgggcctgg cgaacggtgt cgccagcctg gccgagctgt
     1681 acgacctaca gtgggaagac cccggcacga tgcttttcga ctcgccgacg acaggcgaca
     1741 aggttgggcg ctcgtacctg attccgagga cgaaagaaga gctgatgagc gtcggcacga
     1801 tgatgatgcg ctggacgcgg cacacgatgg ggatgatggg ccgcgcgccc gactatatca
     1861 accgcgccat ctcggcctac gccagcgggg ccgacttcct ggcgaagcaa gatgcgcgct
     1921 tcggcgagaa catccgccgg tatcatgaat acgcgcgcga gaacgacctg tgcctgacgc
     1981 ataccttgat caatccacag gcgaatcgcg cggtgggcgc cgcacagcag gccgatccgt
     2041 tcctcgctgc gcgcgtgaaa acagagaccg acgcggggat cgtcgtcaag ggcgcacgca
     2101 tgctcgcgac gctgcccatc tcggacgaga tcatggtctt tccctcgacg ctgctcaaga
     2161 gcggcgagga agacgcgccg tacgccttcg gcttcgccat ccccaactca acacccggcc
     2221 tcaagttcat ctgccgcgag agcgtggact acggacgcag caagtacgac catccactcg
     2281 gctcgcgctt tgaagagatc gacgcgaccg tgatattcga cgacgtgctc gtgccgtggg
     2341 aacgagtttt cttgtaccgc gacgtggccg cctgcaaccg ggcctactcg gcgacgggcg
     2401 cggtggtgca catgtcgcat caggtcgtgt gcaagaacgt cgccaagacc gagttcgtgc
     2461 ttggcctggt gtcgctgatg gtcgacgcga tcggcgtcga gcagttccag cacatccacg
     2521 agaaggtcgc cgaggtgtgg tgcatcctgg aggcgatgaa ggccttcaag cgcgtggccg
     2581 aagtcgacgc cgcgccgaac gagtacggcg tgatgacgcc ggcctgggat ccgctggacg
     2641 cggcgcgcaa catgtatccg cgcctgtatc cgaggctgat cgagatcgtg cagcaggtag
     2701 gcgccagcgg cctggtcagc atgccgacgg aagccgatgt caacggcccg ctggccgaag
     2761 acatccggaa atattaccag gccgcgcgca tgggcgcgct ggagcgcatc ccgctctttc
     2821 gcctggcctg ggatacggcg gtgtcggcgt ttggctcacg gcaggtgctg tacgagcggt
     2881 tcttcttcgg cgacccggtg cgcatggcgg gcgtgctgtt ccagggccac gatcgcaagc
     2941 cgtacatgga catggtgcgc gagttcctca ggcgcagcgc ccgcgatggt gaagaataat
     3001 cgggacgcgg acgcgaagca tggataaacg cggattcgac tttatcttcc gagcgatgag
     3061 ccatccctcc aacagttcga cgatcgaccc gcgcgcgtat cgcaatacca ttggcgcgtt
     3121 cgccacgggc gtgaccgtgg tggccgccgg gcacggcaat catttgcacg cgatgacggc
     3181 caacgccgtc acctcattat cgcttgatcc gccgctgttg atcttctgcg tgagcaagaa
     3241 ggcgaggatc gtcgaagtcc ttgaggcggc ccatggtttc tcggtcaaca tcttgcgcga
     3301 cagccagcag gcgctctcga cgtatttcgc cggaggatgg aaggagccgg cgccgccgcc
     3361 gtttcgcttc gtgccgtggg aaggagggcc gcgcctggaa ggctgcgccg cgtcgatcgg
     3421 ctgcacgctg cacgagcgga tcgagggcgg cgaccactgg atcgtggtgg ggcacgtcgt
     3481 ggcactgcat cagggcgaag agcctcagcg cccgctgatt ttctacggcg gcaagtaccg
     3541 ccacctcgac gcagcgggc
//
LOCUS       DBNHXB010000244         9160 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_897511, whole genome shotgun sequence.
ACCESSION   DBNHXB010000244 DBNHXB010000000
VERSION     DBNHXB010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9160)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9160
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_897511"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..862
                     /gene="coaE"
                     /locus_tag="AC162G_10030"
     CDS             269..862
                     /gene="coaE"
                     /locus_tag="AC162G_10030"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958992.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR5076197.1"
                     /translation="MKYLIGLTGNIATGKSTVARMLVDLGAERIDADALVHDLQRKGT
                     PTYEKIVAEFGPGILQEDGEIDRKALGAIVFDDPARLRALEAIVHPAVSIESRRRMSE
                     AKADVVVYEAIKLIEAGRHTMCDAIWVVTAPRDAQIARLMRDRDLSEAEARQRVDAQP
                     PPEEKLKHATVVIDNGGSLDDTREQVAKAYAAIGLKR"
     gene            910..1425
                     /locus_tag="AC162G_10035"
     CDS             910..1425
                     /locus_tag="AC162G_10035"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5076198.1"
                     /translation="MPTRPLPGTRLLVKRARPVYAEAIAKLVAEATGGRIRPTEDDVI
                     DRMGLRGFFIATAEGNPVGIVGWTAENLVARVEDYIVHPTSMRPVAGRELLVEVEDAA
                     RELECEAALLLVARGASIDAVTFYESCGYSFRPLAEMPEAWRESAEEVVSDDRFVMVK
                     QLRAELVRQPI"
     gene            1441..1635
                     /locus_tag="AC162G_10040"
     CDS             1441..1635
                     /locus_tag="AC162G_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076199.1"
                     /translation="MATLRKWIDQHPRLTAWLVLAVGMVIIVVLSAKDVGLLPTQMLA
                     LVAATIGLAGLCVWIIGWED"
     gene            complement(1714..2718)
                     /locus_tag="AC162G_10045"
     CDS             complement(1714..2718)
                     /locus_tag="AC162G_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076200.1"
                     /translation="MRVSRYRIAPLLLLALLTVLVLAGGLWLISGRRHAGDDYEYFPQ
                     IQHSVRGEFLRFFRENGELEIFGFPMTEEFLFDGRIIQYFQRARMELHPENEEPNRVR
                     LSPLAEELAGKLNLDTGPMNEPLSVSPGDPSQRYFPETGHAVGEPFLGYFDAHGGARI
                     FGNPITEAVQQEGRLAQYFQNMRLDYYPERPSGQQVQPADLGDVHFLLTGMDPSLRKG
                     VKAIAAATPGLSAGPVELRLDAHVARPYAASPEQQVLHVYVTDQDGHSVENAAVEFTV
                     QYPGSPRLYKMPLTNEEGHTSMSFPLEPIPAGQKLMILVTASAGTAAETMLVSSLPWQ
                     "
     gene            3064..3435
                     /locus_tag="AC162G_10050"
     CDS             3064..3435
                     /locus_tag="AC162G_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HIR5076201.1"
                     /translation="MNAILGQCPVCGELLEVTRLHCRACDTTVEGHFNPGKLSQLTPD
                     QLAFVEIFLKNEGKINRVGEELGISYPTVRARLQEVIAALGYQVEEESAIGDEERRQL
                     LDELAQGHISSEEVLKRLGSH"
     gene            3461..3817
                     /locus_tag="AC162G_10055"
     CDS             3461..3817
                     /locus_tag="AC162G_10055"
                     /inference="COORDINATES: protein motif:HMM:NF046909.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT-like domain-containing protein"
                     /protein_id="HIR5076202.1"
                     /translation="MMPESERTQVLKMIEAGKINAAEGARLLGAVSRPPAQSDWSGRW
                     LRVRVTNLSTRQPKVSVNLPITWIMLGLQIGARYHPELAQIDVNEVLEAIRTGAEGRI
                     VEVENEEGERIEVYVD"
     gene            3830..5062
                     /locus_tag="AC162G_10060"
     CDS             3830..5062
                     /locus_tag="AC162G_10060"
                     /inference="COORDINATES: protein motif:HMM:NF046909.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT-like domain-containing protein"
                     /protein_id="HIR5076203.1"
                     /translation="MSERIFDTSPTPHVSVEMCGGDLSLAAVDAPRVLVDLSRNDGDI
                     QREGETLRIRTGGDCKITCPPGASITIEQVGGDLSVQDVKGTLAVRSAGGDVSLRGAG
                     VVTLQSAAGDVSARDIAGDLRIEAIRGDLEVRRVAGQFIAANVGGDLSARALGRGFDI
                     SAVCDVSLEADIQAGETYRARAGCDLTLRVPYDASARFKLSAGGEIGRRIELSEWQGN
                     SHTGQGAMGKGEAEVELVAGGDLMLLPARSKGDDVFGFNFDAIDSQIGAKMEQFERDL
                     DAKMAKLNEQIARMATVGAADLEVRLSRASTERVTRHAERAAEHARRQAEHAAERARR
                     QAERAAERARRRAERSSRWHAAHIHVDIPPRAARPSAPSKPAAPVTEAERLMILRMLE
                     ERKISVEEAGRLLDALEG"
     gene            5063..6130
                     /locus_tag="AC162G_10065"
     CDS             5063..6130
                     /locus_tag="AC162G_10065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5076204.1"
                     /translation="MVASAARRRAPFSGLRPLDAGRDLAAVADLIAEAFQDDMDPAGE
                     RAIRDIRAMGRWALWFGWMDRFAPPGEGMAPGFVWVEDDRIVGNVSVRRGGPLRRGWM
                     IGNVAVAREWRRRGIARALMQAAIELARRHHGDWVALQVRSDGTAPRLLYESLGFAVL
                     GETIHYRRTRPPALVAEALTQTLSGAKGKGKGQRRGAPVAQPGSLGEDRVRRGTARDG
                     ERIYRLAQAAIPEELRWAEPLRRDDFLMGLDRDFANWLSRRREAWWVIESEAEMLGAA
                     HIVMPPPPHEGRLWIGVIPSHQGRYEDHLVHAALSSIESVARRAIFASVPAAHAAARA
                     ALEAAGFEAHRRLTHMRLKLR"
     gene            6200..7462
                     /locus_tag="AC162G_10070"
     CDS             6200..7462
                     /locus_tag="AC162G_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5076205.1"
                     /translation="MSFFTIWIGQAFSLLGSGLVQFVLVWWLTIETGSATVLAVATLV
                     AILPQVVVGPLAGPLVDRLNRRAILIVADGVVALATLWLIYLYSIDAMQVWHVYAAML
                     IRASVGAFHFPTMQASTSLMVPQEHLSRVAGLNQALQGITSILSPALGALLLQIFELR
                     IALAVDVGTALLAILPLFFIAIPQPPRTTTAVAESGRASFGQDLRAGLRYVRGWPGLM
                     AILIMAMLINFLLVPSGSLMPLLVKNELNGDAGLFATMEALLGVGVIVGGIVLSVWGG
                     FKRRVVTSLVGLIGIGVGVIVVGLTPASAAWLAVAGMFVLGAMQSLTNGPLFAVLQTV
                     VAPDMQGRVMSLIGSLSAAMTPLSLIVAGPVADALGVRVWYIVGGVACTLMGVAGFFI
                     PAVINVEHNHVQTATPSTVVDERVVEMT"
     gene            7478..8074
                     /locus_tag="AC162G_10075"
     CDS             7478..8074
                     /locus_tag="AC162G_10075"
                     /inference="COORDINATES: protein motif:HMM:NF046909.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT-like domain-containing protein"
                     /protein_id="HIR5076206.1"
                     /translation="MSEERIKILQMVADGKISAEQASQLLAALGNPPLPPPVLTGGGR
                     GGPEPSPPPPMPHFGSLWLIPMYVGLALFVCGALAVFPFYAASGSWLWLVCGWPVFLI
                     GLLTIFAGWFARKSRWVHIRITNVDGSRRNIKLSFPLPLRLTAWVLKVASRFSGKLKE
                     TGVDEIIVALNEGLKSDQPLIVDVQDDDDGERVQVYIG"
     gene            8229..8609
                     /locus_tag="AC162G_10080"
     CDS             8229..8609
                     /locus_tag="AC162G_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT-like domain-containing protein"
                     /protein_id="HIR5076207.1"
                     /translation="MTTVEERMQILKMIEEGKISAEEGAKLLQALAGKSDKRRTSGAL
                     AGDEARWLRVRVTDLDTGRNTVNVNIPMSLVAVGLKMGARFAPNVEGLDYEQITEAIK
                     SGAQGKIVDVVDEEDGERVEIFVE"
     gene            8621..8986
                     /locus_tag="AC162G_10085"
     CDS             8621..8986
                     /locus_tag="AC162G_10085"
                     /inference="COORDINATES: protein motif:HMM:NF015953.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR5076208.1"
                     /translation="MRRTFFLRCVIGGLGLALAIQLVGGIRYAGGPFEFVLLSLLFGL
                     VSAVLKPILSLLSCPLVLLTLGLFLLVINAFLLQITSSLAQTIDIPFVVVDFGAAFLG
                     GIIISLTGLLANLVISDQQ"
BASE COUNT         1663 a         2781 c         3018 g         1698 t
ORIGIN      
        1 agattacggt gctcgaaacg cgcatcgccg agctatcgca ccagatcgaa aaggccccgc
       61 cggacaaggt cggctctctg ggggaagaat acgcgcgcgt cgagcgagac ctgcaccggc
      121 ggctggatga gtggacagta atcagtgatc agtagccagt gatcagtggt cagtcttcat
      181 caggtccacc gctcactgat aactaatcac tgataactgg tcactgataa ctggccactg
      241 ataaccggtc actgctcgct acgcggtcat gaaatacctc atcgggctaa ccggcaacat
      301 cgcgaccgga aaatcgaccg tcgcgcgcat gctggtcgac ctcggcgccg agcggatcga
      361 cgccgacgcg ctcgtccacg acctgcagcg caaaggcaca ccgacttatg agaagatcgt
      421 cgccgaattc ggccccggca tcctgcagga ggatggcgag atcgatcgca aggcgctggg
      481 cgcgatcgtc ttcgacgatc cggcgcggct gcgcgcgctg gaggcgatcg tgcaccccgc
      541 tgtctcgatc gagagccggc gccgcatgtc agaggcgaag gcagacgtcg tggtctacga
      601 agcgatcaag ctcatcgaag ccggccgcca cacgatgtgc gatgcgatct gggtcgtcac
      661 tgcgccgcgc gacgcgcaga tcgcccgtct gatgcgtgac cgcgacctga gcgaggcaga
      721 agcgcgccag cgcgtcgacg cgcagccgcc gcccgaagag aaactcaagc acgccaccgt
      781 cgtcatcgac aacggcggct cgctcgacga tacgcgagag caggtcgcga aagcttacgc
      841 ggccatcggt ttgaagcgct gatcgagttg tggtatagta tcgtccgttg cccgcagagg
      901 agatagccta tgcccacacg accccttccc ggaacgcggc tcttggtgaa gcgcgcccgc
      961 cccgtctacg cggaagccat cgccaaactg gtcgccgagg ccaccggcgg ccgcatccgt
     1021 cccaccgagg acgacgtgat cgatcggatg ggactgcgcg gattcttcat agcgactgcg
     1081 gagggaaacc cggttggcat cgtgggctgg actgccgaga atctcgtcgc acgcgtcgaa
     1141 gactacatcg tgcatcccac cagcatgcgg ccggttgccg ggcgcgaatt gctggtggag
     1201 gtcgaagatg ccgcgcgaga gcttgagtgc gaagcggcgc tcctgctcgt cgcgcgcggc
     1261 gcgtcgatcg acgccgtgac gttttacgag tcatgcggtt attccttcag gcctcttgcc
     1321 gagatgccgg aggcatggcg cgagtcggcc gaggaggtcg tgtcggatga ccgtttcgtc
     1381 atggtcaagc agttgcgcgc cgagctggtg cgccagccga tttgatgccg aggtgcccag
     1441 atggcaacgc tccgcaagtg gatcgatcaa caccccaggt tgacggcctg gctcgtgctg
     1501 gcggtcggca tggtgattat cgtcgtgctc tccgcaaagg acgtcggctt gctgccgacg
     1561 caaatgctcg cgctcgtcgc cgcgacaatc ggcttagccg gtctgtgcgt gtggatcatc
     1621 ggctgggaag actgaaccgt ccacgcgata caggctccaa tacaaagcgc ccgcgggatc
     1681 aagcctcgcg ggcgctgctg ttattcggcc cgatcattgc cacggcagcg acgagacgag
     1741 catggtctcg gcggcggtgc cggctgaagc ggtgacgaga atcatcagct tctgcccggc
     1801 cggtataggc tcgagcggga agctcatcga cgtatggcct tcctcgtttg tcagaggcat
     1861 cttgtacaga cggggagagc ctgggtactg cacggtaaat tcgaccgcgg cgttttcaac
     1921 cgagtgaccg tcctgatcgg tcacgtacac gtgcaagacc tgctgttcgg gagacgccgc
     1981 atacggcctg gcgacgtgcg catccagccg cagctctaca ggtcccgcag acaagccggg
     2041 tgttgccgct gcgatcgctt tgacgccctt tctcaggctt ggatccatcc cggtgaggag
     2101 aaagtggacg tcgcccaggt ccgctggctg cacttgctgt ccgctcggtc gctccgggta
     2161 atagtccagg cgcatgttct ggaaatactg cgccaggcgt ccctcttgct gaacagcttc
     2221 ggtgatcggg ttgccgaaaa tgcgggcgcc gccgtgcgcg tcaaagtacc ccagaaatgg
     2281 ttcgccgacg gcatgtccgg tctccggaaa gtaacgttgg gaagggtcgc caggcgaaac
     2341 gctcaatggc tcattcatcg gtccggtgtc caggttcaat ttcccggcca attcttcggc
     2401 caacgggctt agacggacgc gattaggctc ctcgttctcc gggtgaagct ccattctggc
     2461 gcgctggaag tactgaatga tgcggccgtc gaacagaaac tcctcggtca tcgggaaccc
     2521 gaagatctcc agctcgccgt tctcccgaaa gaacctgagg aactcgccgc gcacgctgtg
     2581 ctggatctgg ggaaaatact cgtaatcgtc gcccgcgtgc cggcgcccag agatcagcca
     2641 caacccgccg gccaggacga gcacggtaag cagggctaac aatagcagcg gggcaatacg
     2701 atacctggac actctcattc cgtcagccga tcgcgtcccc aaaccgattt ccaaagcggc
     2761 ctgattctac atcagcatat tgcttttggc aatacagttt cggaaccatg cgcacggcct
     2821 ttcttgtgcg accaccggca aggcttgaga tcagaatgat tgagtaagga gccattggtc
     2881 ttcatgttat gtcgagcgca aggcgttctt cgcatttgac catcgtggtt tcaatcggat
     2941 caagaggtcc attatcgaga aagtttattt gcatcttgac aaaattgatt tgtcatgtat
     3001 aatatgttgc gaaaattcac tgcatgattc attttctcaa gggtcgaatc aataatcttc
     3061 actatgaacg caatccttgg acaatgcccg gtatgtggcg agcttctcga ggtcactcgc
     3121 ctgcattgca gagcgtgtga taccaccgtc gagggtcact ttaacccggg gaagctcagc
     3181 cagctcacgc ccgaccaact ggcattcgtc gagatcttcc tcaagaacga aggcaaaatc
     3241 aaccgggttg gcgaagaact cggcatctcc tacccgaccg tccgcgcccg gctgcaagag
     3301 gtcatcgccg cgcttggcta ccaggttgaa gaagagtctg caatcggcga tgaagagcgc
     3361 cgccagctcc tcgacgagct ggcccagggt cacatctcat ccgaagaagt cctcaaacga
     3421 ttagggtcgc actgagtcac accagagaag gagaataacc atgatgcccg aatccgaacg
     3481 cacccaagtg ttgaaaatga tcgaggccgg caagatcaac gctgccgagg gagcgcgcct
     3541 gctcggcgcg gtgagccgac cgcccgccca atcggactgg tctgggcgct ggctgcgcgt
     3601 gcgagtcacc aacctgtcga cgcggcagcc gaaggtgagc gtgaacctcc cgatcacctg
     3661 gataatgctg ggcctgcaga tcggcgcgcg ctaccatcca gaactcgcgc agatcgacgt
     3721 gaacgaggtc ctcgaagcga tccgcactgg cgccgagggg cgcatcgtcg aagtcgaaaa
     3781 cgaggaaggc gagcggatcg aagtctacgt ggattgagcg aggtgggcca tgtctgagcg
     3841 aattttcgac acgtcgccga cgccgcatgt ctccgtcgag atgtgcggcg gcgatctctc
     3901 ccttgcggcc gtcgacgcgc cgcgagtcct cgtcgacttg agccgtaacg atggagacat
     3961 tcagcgcgaa ggcgagacac tgcgcatccg aaccggcggc gactgcaaga tcacgtgccc
     4021 gcccggcgcc agcatcacga tcgagcaagt cggcggcgac ctgagcgtgc aggatgtcaa
     4081 gggtacgctc gccgttcggt ccgccggcgg cgacgtatcg ctgcgcgggg caggcgtcgt
     4141 caccctccaa tccgcagccg gcgatgtgtc ggcgcgcgac atcgcgggcg atctgcgtat
     4201 cgaagccatt cgaggcgacc tcgaagttcg ccgcgttgcc ggccaattca ttgcggcgaa
     4261 cgtcggcggg gacctcagcg cgcgcgcgct cggccgcggt ttcgacatca gcgcagtctg
     4321 cgacgtgtcg ctcgaagccg acatccaggc cggcgagacc tatcgggccc gagccggctg
     4381 cgatctcaca ctgcgcgtgc catatgacgc gagcgcgcga ttcaagctca gcgcgggcgg
     4441 cgaaatcggg cggcggatcg agctgagcga gtggcagggc aactcccata ccggccaggg
     4501 cgccatgggt aagggtgaag cagaggtcga attggtcgcg ggtggcgatt tgatgctgct
     4561 gcccgcgcgc tccaagggcg atgacgtctt cggcttcaac ttcgacgcga tcgacagcca
     4621 gatcggggcg aaaatggagc agttcgagcg ggacctcgac gccaagatgg cgaagctcaa
     4681 cgaacagatc gcccgaatgg ccaccgtcgg cgcggccgac ctggaggtgc gcttgagccg
     4741 cgcctccacc gagcgcgtga cccggcacgc tgagcgcgcc gccgagcacg cgcgccggca
     4801 ggccgaacac gcggccgagc gcgcgcggcg acaggccgag cgagcagccg agcgcgcacg
     4861 ccggcgggcc gaacgcagca gccggtggca cgctgcgcac atccatgttg acatcccgcc
     4921 acgagccgcc agaccgtccg cgccttccaa gcccgccgcc cccgtcaccg aggcggagcg
     4981 cttgatgatt ctgcgcatgc tggaagagcg caagatcagc gtcgaggaag ccggccggct
     5041 gctcgacgca ctggagggct gagtggtcgc cagcgccgcg cggcggcggg cacccttctc
     5101 cggcctccgg cctctggacg ctggccgcga cctggcagcc gtcgccgatc tcatcgccga
     5161 agcctttcag gacgatatgg atccggccgg cgagcgtgcc atccgcgaca tacgcgcgat
     5221 gggacgctgg gccttgtggt tcggctggat ggatcgcttt gcgccgccgg gcgagggcat
     5281 ggcgcccggt ttcgtgtggg tcgaagacga ccgaatcgtg gggaacgtct cggtgcggcg
     5341 cggcggtccg ctcaggcgcg gctggatgat cggcaacgtg gccgttgcgc gcgagtggcg
     5401 ccggcgcggc atcgcccggg cgctcatgca ggccgccatc gagctggctc gccggcatca
     5461 cggcgattgg gtggcgcttc aggtgcgctc ggacggcacg gcaccacgat tgctgtacga
     5521 atcgctgggg ttcgccgtcc ttggtgagac cattcactac cggcggactc gcccgcctgc
     5581 cctcgtagcc gaagccctga cccagaccct gagcggagcg aaggggaagg ggaaggggca
     5641 gcggaggggg gcgccggtcg cgcagcccgg atcgcttgga gaggatcgcg tgcggcgtgg
     5701 cacggcgcgg gacggggagc gcatttaccg attggcacag gcggctatcc cggaagagtt
     5761 acgctgggcg gagcctctgc gccgcgacga tttcttgatg ggattagatc gcgacttcgc
     5821 caattggctg tcgaggcgac gcgaggcatg gtgggtgatc gagtcagaag ccgagatgct
     5881 cggcgcggcg cacatcgtga tgccaccccc gccgcatgag gggcgactgt ggatcggggt
     5941 catccccagc catcagggcc ggtatgaaga tcatcttgtg cacgcggcgc tctcttcgat
     6001 cgagagcgtg gcgcgcaggg cgatcttcgc cagcgtgccc gccgcgcacg cagccgcacg
     6061 cgcggcgctc gaggccgctg gcttcgaggc gcaccgccgg ttgacgcaca tgcgccttaa
     6121 gctgcgctga actcacgtgc gacgcacgta agagagccca tacctgatgc aaaacataca
     6181 accttcatcg aattggaaat tgtctttctt tacgatctgg atcgggcagg ctttctcgct
     6241 gctgggcagc gggctagtgc agtttgtgct ggtgtggtgg ctgacgatcg agaccggctc
     6301 ggcgacggtg ctggccgtcg ctacactcgt cgctatcctg ccacaagtgg tcgtcggccc
     6361 gctggccggg ccgctggtcg atcgcctgaa ccggcgcgcg attctgatcg tcgccgatgg
     6421 cgtcgtcgcg ctggcgacgc tgtggctgat ctacctctac tcgatcgatg cgatgcaggt
     6481 gtggcacgtc tacgcggcga tgctgatccg cgcgtcggtg ggcgcgttcc acttccccac
     6541 tatgcaggcc tcgacttcgt tgatggttcc gcaggaacac ctgtcgcgtg tggcggggct
     6601 caatcaggcg ctgcagggta tcaccagcat cctgtcgccg gcactgggcg cactgctgtt
     6661 gcagatcttc gagctacgca tcgcgctggc ggtggacgtg ggcactgcgc tgctggcaat
     6721 cctgccgcta ttctttatcg cgattccgca gccaccgcgc accacaacgg cagtggcgga
     6781 gagtggcagg gcttccttcg ggcaggatct ccgcgcgggc ctgcgttatg tgcgcggctg
     6841 gcccggcttg atggcgatcc tgatcatggc catgctgatc aatttcctgt tggtgccatc
     6901 aggttcgctg atgccgctcc tggtgaagaa tgaactgaac ggcgatgcgg gcttgtttgc
     6961 gacaatggag gcgctgctgg gtgtgggcgt gatcgtcggc ggtattgtgt tgagcgtgtg
     7021 gggtggcttc aaacgccgcg tggtgacctc gctggtgggg ttgataggca ttggcgtggg
     7081 cgtgatcgtg gtcggcctca ccccggccag tgccgcgtgg ctggctgtcg caggtatgtt
     7141 cgttttaggt gcgatgcaat cgctgacgaa tgggccgctg tttgccgtgt tgcagaccgt
     7201 ggtcgcgccc gatatgcagg gccgcgtgat gtcgctgatt ggcagtctgt cggcggcgat
     7261 gacgccgttg agcctgatcg tcgccgggcc ggtggcagat gcgctgggcg tgcgcgtctg
     7321 gtatatagtc ggcggtgtgg cgtgcacgtt gatgggtgtg gccggcttct tcatcccggc
     7381 cgtcatcaac gtggaacata atcacgttca aacggcaacc ccgtcgacgg tagtcgatga
     7441 gcgcgtggtc gagatgactt gataaggatc aaggcacatg tccgaagagc gaatcaagat
     7501 cttgcaaatg gtcgccgacg gcaaaattag cgccgagcag gcgtcgcagt tgctggcggc
     7561 gctgggcaac ccccctctcc ctccccccgt tctgacgggg ggagggagag gggggcctga
     7621 gccatcgccc ccgccgccga tgccgcactt cgggagcctg tggctcatcc cgatgtacgt
     7681 ggggctggct ctgttcgtat gcggcgcgct ggcggtcttc ccgttctacg ccgcgagcgg
     7741 gtcgtggctg tggctcgtgt gcggatggcc cgttttcctt atcggcctgc tgacgatatt
     7801 cgccggatgg ttcgcgcgga agtcgcgctg ggtccacatc cgcatcacga acgtcgacgg
     7861 ctcccggcgc aacatcaagc tcagttttcc attgccgctg cgcctgaccg cctgggtgct
     7921 gaaggtcgcg tcgcgtttct cgggcaagct gaaagaaacc ggcgtcgacg agatcatcgt
     7981 cgcgctgaac gagggtctca agagcgacca gccactgatc gtcgacgtgc aggacgacga
     8041 cgacggcgag cgcgtacagg tgtatattgg gtagtgccat gtgccacgtg cagcggctca
     8101 cactgcccgc acttggactt ccgattggag tcgaggcttt agccggaccc gcctaaaggc
     8161 tcgactccgg cgctcggaag ttcagcgtgg cctggtaaac gttttacgca tgatgcgtga
     8221 gggtcaacat gacgacagtc gaagaacgca tgcagattct gaagatgatc gaggaaggca
     8281 agattagcgc agaggaaggc gcgaagctcc tgcaggcgct cgccggcaag agcgacaagc
     8341 gccggacgag cggcgcgctc gccggcgacg aggcgcgctg gctgcgcgtg cgcgtcaccg
     8401 acctggacac cggccggaac acggtgaacg tcaacatacc gatgagcctg gtcgccgtcg
     8461 gcctcaagat gggcgcgcgc ttcgcgccga acgtcgaggg cctggactac gagcagatca
     8521 ccgaggccat caagtcgggc gcgcagggca agatcgtcga cgtcgtcgac gaagaagacg
     8581 gcgagcgcgt cgagattttc gtcgagtagc ggcgaaaacc atgcgtcgaa ccttcttcct
     8641 tcgctgcgtg atcggcggct tggggctggc actggcgatc caactggttg ggggcattcg
     8701 ctacgcgggc ggcccattcg agtttgtcct cctgtcgctg cttttcggcc tggtcagcgc
     8761 cgtgctcaag cccatcttga gcctgctctc ctgtccgctc gtgctgctta cgttggggtt
     8821 gttcctgtta gtgatcaacg cctttctcct gcagataacc agctcgctgg cgcagacgat
     8881 cgacattcca ttcgtcgtcg tcgacttcgg cgcggctttc ctgggaggga tcatcatcag
     8941 cctgacgggc ttgctcgcga atctcgtgat ttccgaccaa caataacacg gcattcgccg
     9001 cctgccttcg tagcgaagct gagggggctc gccatcagat tgcgtcacac cgagcaggag
     9061 atcctcacgt tttacgtttg acgttttacg ctttacgttg agccgtgttc gccatcggac
     9121 tgcgtcacac cggggaggag atggcatgga aggtcaagta
//
LOCUS       DBNHXB010000245         5413 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_60116, whole genome shotgun sequence.
ACCESSION   DBNHXB010000245 DBNHXB010000000
VERSION     DBNHXB010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_60116"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1609)
                     /locus_tag="AC162G_10090"
     CDS             complement(<1..1609)
                     /locus_tag="AC162G_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076209.1"
                     /translation="MLAALPLILLAPIVFGGQVLYWGVPLMQFYPWQHYAADMWRSGA
                     VPLWNPLLGNGAPLAANLQTGAFYPLNFLYLLVPIEIAMGYTAVVHLVLAGLFMYAYA
                     RSIGLNPLSALIAAIAFQLSGYLVSRLGFLSITAALPWVAAWLWRAERLMRTGRSLDV
                     MFLAVVVGLGLLAGHAQTSVHGLILLASYTLYRGLSTREWRRMLSAGAGMLLGLGLAA
                     IQLVPSAELARESQRVGGLDYTFAMTHSYWPWRLLTLLAPDLFGNPADGDFWGYDNYW
                     ENAAYIGVLPLALAVLAILEVPWSLWKRRARNFKLQTPNSKLQTPTPNPQSLIPNYQS
                     LTLFLATSATLLLILAFGWFTPIYPFLFRTIPGFELFQGPARWLGIFTIALCALAGIG
                     AERMLRGRGNLRLSGVWIVVGAAVAAAGLVAGLFLTGRVATFPDAALRLGVLVAIGGL
                     LFGLRPPAGAQRFAWWAGAFVAVIALDLLTAHARLNPTIDPELYRLPSSSAEALRTGD
                     GRVFMFNDDDAAVRKQYGISLPFSSFGP"
     gene            complement(1666..3933)
                     /locus_tag="AC162G_10095"
     CDS             complement(1666..3933)
                     /locus_tag="AC162G_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076210.1"
                     /translation="MRESRRWRRDAAFASMIVLIAVLALLLPALLQPDGLIYPPQGSF
                     TDLTITHWPAFDYLSASLRQTGALPLWRTSILGGTPFAADPVTGTWYLPNWLSIALPL
                     EAFFKLIFAAHLALGGVAMMFLARSFGVGWIGAAASGVAYGVAPRVVAHMGAGHVTLV
                     EAWPWMPLTVWAYRRGATWKGALFSGVALGFCALADLRAVVYAGVVIAAYALIAGDAS
                     RALRVRVLHLLIILSAALFVGAAAWLPALSLAGESTRASLSAGEAGAYSLPFEYLAGV
                     LLAVRGDAERLTYVGLAVLVLALAGLQTLLREQRRAAIWLITLVVIGSIAALGANTPL
                     YDVMVRLPGVSLLRVPGRAWFLVAFAAGLACGLGVDAMVKRKETRLSKPWMLAALLVA
                     WFALLFGLGGALLAYTSGQAGAGRAGLSLIGLAVVLPLAIALVIARASGRLAPSRFGA
                     LVVVLIAIDLVWVGWGQYRVVSRNEAFTDGRAVAAYLASETQEALFRVYSPSYSLPQH
                     AAQEYELELADGIDPLQLERTVRFMQRASGVGEWGYSVTLPAFEELTRDEDVRTLLSG
                     VVPDPALLGLLNVRYVVAHFPIAHADLIEIARIDGAYVYKNARVSRHAWVAGRVDMVS
                     NQAQALDWLDSHDMRGEAVVEGGRPLSLGVVGGEARIVARAPDRLVVSARGPGLLVVS
                     EVYERNWRALVDGEAAPIYAVDGVLRGVYLDDGQHLVEFDYDPAAVKLGVLIGGAAIV
                     IWLSIWVITQRRQVA"
     gene            complement(4017..>5413)
                     /locus_tag="AC162G_10100"
     CDS             complement(4017..>5413)
                     /locus_tag="AC162G_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076211.1"
                     /translation="TDGQSYFAKMRQGHEGSWLFRLPYTHNDEADAFLFTYHLGLGHI
                     ARITRLPLTVVYHAARIIGGWVLLVVAYVLLARVFDATAERRRAWWFVALTSGLGWIG
                     LRGTDLVIPESNTFFAVLTNAHFALAMALMALLFMAVVDGSVLMAVLTSVALAVLQPF
                     APIAVFAALGVFLLFRWVRPLEDKRGRTFPMRQARAVLAAGLIITPLMLYFYLVTQRD
                     PILQGWSTQNVTPSPQVGEYLIGFGVMSILAIPGARFALKRGRDTDVIVVAWAVTSAL
                     LLYAPFALQRRFSLGLHIPVALLGVMGLTYVLAPRFRDRAAHRLPIVLFAISLPSTLL
                     LLLATSTAAFVQPPDARLFVTADEAASFQWLRQNVPHESVVIAAPETGLFLPGWAGVR
                     VLYGHPFETLDAEKMKATVEQFFSGSVDRADVIRDYGVDFIFFGPHERNLGTPEPEWE
                     AAFVAGDVAIYKAP"
BASE COUNT         1190 a         1787 c         1669 g          767 t
ORIGIN      
        1 ccggaccgaa agacgaaaac ggcagggaga tgccgtactg cttgcgcact gcggcgtcgt
       61 cgtcattgaa catgaagacg cggccatcac cggtgcgcag cgcctccgcc gatgacgagg
      121 gcaggcgata tagttccgga tcgatcgtcg gattgagcct ggcatgcgcc gtgaggaggt
      181 cgagggcgat gaccgccacg aaagcgcctg cccaccaggc gaagcgctga gctcctgccg
      241 gtgggcggag gccgaacaac agcccgccga tcgcgacgag cacccccagt cgcagcgcgg
      301 catccgggaa ggtcgccaca cgcccggtga gaaagaggcc ggcgaccagg ccggcggcag
      361 ctacggcagc gccgacgacg atccagacgc cactgagacg caggttgccc cgcccacgaa
      421 gcatgcgctc cgcgccaatt ccggcgagcg cgcagagcgc gatggtgaat atgcccaacc
      481 agcgcgccgg accctgaaac aactcgaagc ccggaatcgt gcggaacagg aacgggtaga
      541 tcggggtaaa ccagccgaag gcgagaatca gtaggagggt cgcgctcgtt gcaagaaaga
      601 gggtcaggga ttggtaatta gggattaggg attgaggatt gggggtggga gtttggagtt
      661 tggagtttgg agtttgaagt ttgaaatttc gcgctcgtct cttccagagg ctccacggaa
      721 cttccagaat agccagcacg gccagcgcca gcggcagcac gccgatatat gcagcatttt
      781 cccagtagtt gtcgtaaccc cagaagtcgc cgtcggccgg gttgccaaac agatccggcg
      841 cgagcagcgt gagaagccgc cacggccagt atgaatgcgt catggcgaac gtgtagtcca
      901 acccgccgac gcgctgcgat tcgcgcgcca gctcggcgga cggcacgagc tggattgccg
      961 caagccccag tcccaggagc atgcctgcgc ctgccgagag catgcgacgc cattctcgcg
     1021 tcgagagacc acgatacagc gtgtatgagg ccaacagaat aaggccgtgc acggatgtct
     1081 gagcgtgtcc cgcaagcaga cccaggccga cgacgaccgc caagaacatc acgtcgaggg
     1141 agcgtcccgt gcgcatcagg cgctcggcgc gccacagcca cgccgccacc cagggcagcg
     1201 cggccgtgat gctcagaaaa cccagccgcg agaccaggta gccgctcaac tgaaaggcaa
     1261 tcgccgcgat caacgcggaa agcggattca gaccgatcga gcgcgcgtac gcgtacatga
     1321 aaagtccggc cagaacgagg tgcacgaccg ccgtataccc catcgcgatt tcgatcggca
     1381 cgagcaagta aagaaaattc agcggataga acgcgccggt ctggagattg gcggcgagcg
     1441 gcgcgccgtt gcctagcagc gggttccaca gcggcaccgc gcccgagcgc cacatgtcgg
     1501 cagcatagtg ctgccaggga tagaactgca tgagcggcac accccaatac aggacctgcc
     1561 cgccgaagac aatcggcgcg agcaggatga ggggaagcgc ggcgagcacg gcgccaattg
     1621 cgaggtgcga tgtgtgacga gtgatacgtg atgcgtatgc gcgcattagg ctacctgtcg
     1681 tctctgggta atcacccaga tcgaaagcca gatgacgatc gcagcgccgc cgatcaggac
     1741 ccccagcttc accgccgccg gatcgtagtc aaactcgacc agatgctgtc cgtcatcaag
     1801 atacacgcca cgcaacacgc cgtcgaccgc atagatcggc gccgcttcgc cgtcgaccaa
     1861 cgcgcgccag ttgcgctcat atacttcgct caccaccagt agtccggggc cgcgcgccga
     1921 caccacgagc cgatctggcg cccgggcgac aatccgggct tctcctccga cgacacccag
     1981 gctgagcggg cgcccccctt cgacgactgc ttcgccgcgc atgtcgtgtg agtccagcca
     2041 gtcgagcgcc tgggcctgat tactcaccat gtcgacgcgg cccgccaccc aggcgtgccg
     2101 agacacgcgc gcattcttgt acacgtatgc gccgtcgatg cgcgctatct cgatcagatc
     2161 ggcatgcgcg atcggaaaat gcgcgacgac gtagcgcacg ttgagcaaac ccagcagagc
     2221 cggatcgggc acgacgccgg acagcaacgt gcgcacgtcc tcgtctcgag tcaactcctc
     2281 gaaggcaggc agggtcaccg agtagcccca ctcgcccacg ccgcttgcac gctgcatgaa
     2341 gcgcacggtg cgctcgagct gaagaggatc gatgccgtcg gccaattcca gctcgtattc
     2401 ttgcgctgcg tgctggggca aactatacga cggcgagtac acgcggaaga gcgcctcttg
     2461 agtctccgac gcgagatacg cggccaccgc gcggccatcc gtgaacgcct cattcctgga
     2521 cacgacgcgg tactgccccc accccaccca taccaggtct atcgcgatca ggaccaccac
     2581 aagcgcgccg aagcgcgacg gtgcgaggcg cccgctcgcg cgagcgataa ccagcgcgat
     2641 ggcgagaggc aagacgacgg ccaggccgat caggcttaag ccagcgcgtc ctgccccggc
     2701 ttgcccactc gtgtatgcga gaagcgcgcc ccctaagccg aacagcaatg caaaccatgc
     2761 cacgagcaag gcggcgagca tccagggctt gctcaaacgc gtttctttgc gtttgaccat
     2821 cgcgtcgaca cccaggccgc atgccaaccc ggccgcgaac gcgaccagga accaggcgcg
     2881 gcccggcacg cggagcagtg acacgcccgg cagccgaacc atcacgtcgt acagcggcgt
     2941 gttcgcgccc agcgcagcga tcgaacctat caccaccagg gtgatcagcc agatcgcggc
     3001 tcggcgctgc tcgcgcagca gcgtctgcag gccggccaga gccaacacca ggacagccag
     3061 gcctacgtat gtcaatctct ccgcatcgcc tcgaacggcc aggagcacgc cggcgagata
     3121 ctcaaagggc agagaatatg cgccggcttc acctgccgaa aggctggcgc gtgtcgactc
     3181 accggcgagg gagagcgccg gcagccacgc tgccgcgccg acgaagaggg ctgcactcaa
     3241 gatgatcaac aggtgcaata ccctgacgcg cagcgctcgc gatgcgtcac cggcgatcag
     3301 cgcgtaggcc gcgatcacga cgcctgcata cacaactgcc cgcaggtccg ccagtgcgca
     3361 gaatccaagc gcgacgcccg agaagagcgc gcctttccag gtggcgccgc ggcgatacgc
     3421 ccatacggtc aggggcatcc acggccatgc ctcgacgagc gtcacatgtc ccgcgcccat
     3481 gtgcgcgacg acgcgtggcg cgacgccgta cgccacgccc gacgccgccg ctccgatcca
     3541 gcccactcca aacgagcgcg cgagaaacat catcgccacg ccgccgagag ccaggtgcgc
     3601 ggcaaaaatc agcttgaaga acgcctccag aggcagcgcg attgacagcc agttcggcag
     3661 gtaccacgtg cccgtcacgg ggtcggctgc aaacggcgtc ccgccgagga tcgacgtgcg
     3721 ccacagcggc agcgcgcctg tctggcgcag cgatgcactg agataatcga aagccggcca
     3781 gtgggtgatg gtgagatcgg tgaacgagcc ttgcggcgga tagatcagcc cgtccggctg
     3841 cagcagcgcc gggagcaata acgccagcac ggcgatcaga acgatcatcg atgcgaaggc
     3901 agcgtctcgc ctccagcgcc tcgattcgcg catcacaccc tctcaatcac gtgtcacacc
     3961 gatttcctgg ggacgttttt tggctccgaa attgggaata cctgccaatt cgtgtgtcac
     4021 ggagccttgt agatcgcgac atcgccggcg acaaaagccg cctcccactc cggttcaggt
     4081 gttccgagat tcctctcgtg tgggccgaag aagatgaaat cgacgccgta gtctcgaatc
     4141 acgtcggcgc ggtccacgga acccgagaag aactgctcga ccgtcgcctt cattttctcc
     4201 gcgtcgagcg tttcaaacgg atgcccgtac agcacgcgca cgccggccca gccgggcagg
     4261 aacaaccctg tttccggcgc agcgatcacg actgattcgt gaggcacatt ctgacgcagc
     4321 cactggaacg acgctgcttc gtccgccgtg acaaacaggc gggcgtcggg gggctgaacg
     4381 aatgccgccg tcgaggtggc caacagcagc agcagagtgg acggcagcga gatggcgaac
     4441 aagacgatcg gcagcctgtg cgccgcgcga tcccggaatc gaggcgccaa cacataggtc
     4501 agtcccatga cgcccagcag ggcgacggga atatgcaggc caagcgagaa gcgccgctgc
     4561 aacgcgaacg gcgcatacag gagcaaggca ctggtgaccg cccaggctac cacgatcacg
     4621 tcggtgtcgc gcccgcgttt caacgcaaag cgtgcgcccg ggatcgccag gatcgacatc
     4681 acaccgaaac ctatcaggta ttcacccacc tgcggcgaag gtgtgacgtt ctgcgtcgac
     4741 catccttgca agatcggatc gcgctgtgtg accaggtaga aatacagcat cagcggcgta
     4801 atgatcaatc cggccgccaa cacagctctc gcctgtctca ttggaaatgt ccggccacgc
     4861 ttatcttcca gtggtctcac ccacctgaaa agcaagaaaa ctccgagcgc cgcaaacacg
     4921 gcaataggcg cgaacggttg aagcacggcc aacgccaccg aggtcagcac ggccatcaga
     4981 accgatccat cgaccacggc catgaagagg agcgccatga gcgccatggc aagggcaaag
     5041 tgcgcattcg tcagtaccgc gaagaaggtg ttcgattcag gtatgaccag atcggttccg
     5101 cgcagcccga tccagcccag gcccgacgtg agcgccacaa accaccatgc ccgtcgtcgt
     5161 tcggcggtcg catcgaagac acgcgccagc aacacgtagg ccacgaccag cagcacccac
     5221 ccgccgatga tgcgcgccgc atgatacacc accgtcaagg gcagacgcgt gatgcgagcg
     5281 atatggccca atcccaggtg atatgtgaac aagaacgcgt cggcttcgtc gttatgcgtg
     5341 tacggcaacc gaaacagcca ggagccttcg tggccctgac gcatcttggc gaaatagctc
     5401 tgcccgtcgg ttg
//
LOCUS       DBNHXB010000246        16677 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_60313, whole genome shotgun sequence.
ACCESSION   DBNHXB010000246 DBNHXB010000000
VERSION     DBNHXB010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 16677)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16677)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16677
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_60313"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /gene="uvrA"
                     /locus_tag="AC162G_10105"
     CDS             <1..1496
                     /gene="uvrA"
                     /locus_tag="AC162G_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR5076212.1"
                     /translation="ERQRAIARQILKEIRARLQFMVDVGLDYLTLSRTASTLSGGEAQ
                     RIRLATQIGSQLMGVLYVLDEPSIGLHQRDQARLIKTLQAMRDLGNTLLIVEHDHDTM
                     EAADWIIDLGPGAGVHGGEVIFEGTVKQLKADRKSITGAYLSGRTKVEMPAQRRVGNG
                     KFLVVRGATENNLKKINVSIPLSKFVCVTGVSGSGKSTLVIEVLFKRLAQMLHGAHEA
                     AGAHIGLKGVEHIDKIINIDQSPIGRTPRSNPATYTGFFGDIRDIFANLPESKVRGYG
                     PGRFSFNVKGGRCEACEGQGQVQIEMQFLPDIFVPCEVCHGARYNRETLQVRYKGKNI
                     AEVLSMTVEEALAFFENIPPLRRKLETLSRVGLGYITLGQPATTLSGGEAQRVKLSRE
                     LSKRSTGRTLYVLDEPSVGLHAADVHLLIDVLQQFVDAGNTVLIIEHHPDIIKVADHI
                     IDLGPEGGEAGGRIVAEGTPEEIAQVRESHTGQFLKRLLGNGSRSRQ"
     gene            1723..2985
                     /locus_tag="AC162G_10110"
     CDS             1723..2985
                     /locus_tag="AC162G_10110"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR5076213.1"
                     /translation="MSTSSSAPNAYQVARQQFERAAVHLEDIPPGIIEFLRHPKRELT
                     VNFPVKMDDGRLRMFTGYRVHYNNVLGPTKGGIRFHPDVTLDEVRALAMWMTWKCSLM
                     NIPYGGAKGGVAVDPAQLSPGELERLTRRYATDISILMHPSNDVPAPDVGTNPQIMAW
                     IMDTYSMHFGYIQMAVVTGKPIEVGGSLGRTDATGRGVMITAREAMNHLGIPIPGARV
                     AVQGFGNVGSVAAHTAYEMDCKVIAASDVGGGIVDKGGLHVPSLVEHVRKTGSVVNFP
                     GTEPIDNASLLELECEVLIPAALENQITQDNAANIEAGIVAEGANGPTTNEADAILDA
                     NGVFVIPDILCNGGGVTVSYFEWVQDLQSFFWDEEEIARRLERTMVKAFHDVLRFSQE
                     RKINLRTAAQALAIRRVAQAAMIRGIYP"
     gene            3088..4215
                     /gene="queG"
                     /locus_tag="AC162G_10115"
     CDS             3088..4215
                     /gene="queG"
                     /locus_tag="AC162G_10115"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIR5076214.1"
                     /translation="MTLTARIRGRATDLGFDLIGVAPAGRALRADAYVQWVEAGRAGE
                     MEYMTRDPQRRQDARHFMPEARSVVVVGLSHYTVNLPDEIKRDPSRGLIARYAWGLDY
                     HDIVTPRLRELLAFIRAEARREIQAKVYVDTGPVLERDFAERAGLGFTGKNTCLIHPR
                     TGSWLFLGEIILDLELDYDVPVTSIGCGSCTRCLSACPTDAFVAPYVLDARRCISYLT
                     IELKGNIPRELRPPLGNRIFGCDECQDVCPWPRRFARPTSEKAFYPIDFERAAPKLLD
                     VIGLSAEQFRERFRGTPILRAKRKGLLRNAAIAIGNWGDETAVPGLVNALNDPEPLVR
                     AHVAWALGEISTLAARNALETQAKIEPDPSVQQEIADALIR"
     gene            complement(4240..4491)
                     /locus_tag="AC162G_10120"
     CDS             complement(4240..4491)
                     /locus_tag="AC162G_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076215.1"
                     /translation="MKLPAATYEIRVEGHLDDHWSAWLGGMTLTRTGRSETILTGALP
                     DQAALYGVLMKMRDLALTLVSIRRIESDNSNPTRASDEG"
     gene            complement(4488..5387)
                     /locus_tag="AC162G_10125"
     CDS             complement(4488..5387)
                     /locus_tag="AC162G_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5076216.1"
                     /translation="MKDKTILITGATSGIGFHTAQALAAKGARVIVTGRDEKRGREAI
                     DELRRRAGHDQVHFVAVDHSTVGGNGDLADRVAKELDRLDGLINNVGGSPNGQRQETA
                     DGYEASLAMNFVGPVALTRGLLQLLRESAPARIVNVVSSAHAMWKRDPFEDLDARERY
                     VGIEAHAHAKLLNLLWTFALARRLEGSGVVVNATNPGMAWTPSTQALTPQAVPAWRFT
                     WPLVRWIQRRASAEAASRSSVFLASSDDAASVSGMYFESDAKPKRPSALALDIGNQER
                     AWELAATLVARAPTARFAQKSGV"
     gene            complement(5522..5986)
                     /locus_tag="AC162G_10130"
     CDS             complement(5522..5986)
                     /locus_tag="AC162G_10130"
                     /inference="COORDINATES: protein motif:HMM:NF021538.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HIR5076217.1"
                     /translation="MFPIYNLFEFLHIAAFIVWIGCVVALSIINVRLAREQDRAALVA
                     LSRQSDFYGRAILGPAAGITLITGIVMVAVLGLGFGTLWIVWGLVGVFGSLVLGVALI
                     RRATAELSALAPTVAPNDPRVIALQQRLATLSTINLLLLLSAVWAMVFKPAL"
     gene            complement(6183..8909)
                     /locus_tag="AC162G_10135"
     CDS             complement(6183..8909)
                     /locus_tag="AC162G_10135"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5076218.1"
                     /translation="MVLLATKLYLPAARTGSRIVPRPRLIERLNEALARPLLLISAPA
                     GCGKTTLLGDWIAHNHRRVAWLSLDEGDNDPTRFWTYFIAALQTVQADFGESVQAILQ
                     ETGQQPIPLESFLTVLLNEMAASREGFALVLDDYHVVTTPAIHEGLTFLLDHLPPETQ
                     VIIASRADPPLPLARLRASNRMAEFRADDLRFTPDEVAVFLNQVMGLDLSADDIAALE
                     ARTEGWIAGLQLAALSLLGRDETAKRHFVSTFTGSQRYILDYLVEEVLQRQPETTQAF
                     LLQTSILARLSGSLCDAVTGRADGQAMLEELERANLFLIPLDEDRRWYRYHHLFAEAL
                     RLRLQQAAPGRVAEIHRKAATWSEEHGLIDDAVHHALEAGDTAWAAQLVEQHVEEVLS
                     RGEGETLRRWLAAVTQAAVRSRPRLALAQAIAAFNTGRLEAVEPLLEDAERALATTPS
                     EPYTPSIGREVSMLANVPATIALLRASLVGLRGDAERMTELVRLALTHLTEDERGPHI
                     SVRWNLALADWMRGRLAEAERAFAGIVAEGRVAGEPHLMLSAGSVLGRVQRAQGRLDA
                     ALRTYQKGLEFAAQTGSPVVLSAAMAHMGMAEVLYERNQLEQALRHVTEGISLGRQLT
                     STQSLATGLATLAWIRQARGDPAGALEAMDEAYQVMPALEVVALHNPVPAERARLWLA
                     QGDVMEAARWVEAQGLEEDAELSYPRECEYLVLARVLLARNMSDRALSLLERLGSVAE
                     AQARTGSVIEVRVLKALALKAIGEQARALAVLVEALTLAGPEGYVRVFVDEGKPVAGL
                     LQQVTGEQWPYAERLLAVLAKSEQGSVEAPTHPPVSPPIHAQSPALIEPLSPREMEVL
                     RLIAAGLSTAQIADELVITVGTVRNHLKNIYGKLDTHSRVQAVERARALKLL"
     gene            complement(9061..9192)
                     /locus_tag="AC162G_10140"
     CDS             complement(9061..9192)
                     /locus_tag="AC162G_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076219.1"
                     /translation="MSIWKGRISALERLAPVIAAVYVSVWLVMSGIEGHFLHGSRER"
     gene            9512..10216
                     /locus_tag="AC162G_10145"
     CDS             9512..10216
                     /locus_tag="AC162G_10145"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR5076220.1"
                     /translation="MAHRGNSAAAPENTLAAFRRAIDDGADIIETDLHLTRDGVFVCI
                     HDATLDRTTDGHGAVADLTLAEIKRYSASYGRPEFAAERVPALGELTAILPPDVVLAL
                     ELKTDRFLERGVAPRLIDQLRADGVFDRVIVLSFGLERIQAVQRAAPEIPIGYITLTR
                     LTPSVPAQMIGPFWPLLAVNPLYTWWAHRRGMLIAPLDPWPNGRLWLYRLLRCDAILT
                     NDPAGTMKKLGRTLKS"
     gene            10350..11279
                     /gene="mtnP"
                     /locus_tag="AC162G_10150"
     CDS             10350..11279
                     /gene="mtnP"
                     /locus_tag="AC162G_10150"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HIR5076221.1"
                     /translation="MPKLESVQLAIIGGSGLYKMESLAGIKEYRIETPFGDPSDAIVV
                     GTLSGRRVAFLPRHARGHRVLPSEINARANIFALKMMGVTHIVSVSACGSLREHIHPR
                     HVVVPDQLFDRTRGRPLTFFGNGIVVHIGLADPFCPNLSALMAQAVEASGGTVHRGGT
                     FVTVEGPRFSTKAESRAFRQLGFDIIGMTAIPEANLAREAEIAYAAMAHVTDFDVWHA
                     SEEAVTVEMVIRTLNENVALAKEAIQRIAAADLPPSDAHSALSSAIITPAHLIPDKVK
                     RALAPIVGKYLPARPRSQAPRAQRRGKSRQRKA"
     gene            11345..12631
                     /locus_tag="AC162G_10155"
     CDS             11345..12631
                     /locus_tag="AC162G_10155"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR5076222.1"
                     /translation="MQDTLSFRRNLLARPRARERALLLVGGAFVVAGLVQLSIVDPLS
                     MGASLYLAASVVWALVFVVAHLALDRYLPGRDPLILPIAALLTGWGLIEVARLSRTDA
                     LRQLMWVVVSVGVMLIVAAAPRELRWLRRYRYTWLLGGLVLLAATLVFGVNPSGIGQE
                     LWLGFGGIFFQPSEVVKLLMVVFLASYLAEKRDLQLVMRIQSRSLTVAYLAPMLAMWG
                     VAMTILLIQQDLGAAMLLFLAFLVMLYLASGKWQYLLIGMVLLACAVLIGYALIGRVT
                     ERVNTWLDPWSRAEVESYQVVQSLIGIGAGGLFGRGLGQGDPVVPAAHTDMPLAVIGE
                     EFGLLGAIGLVACYALLTLRGLRAAALSRTVFGCLLAAGLAALLGMQAWIIMAGNINL
                     IPLTGVTLPFVSYGGSSLVVSFVMVGLLLHVSANAD"
     gene            12790..13410
                     /locus_tag="AC162G_10160"
     CDS             12790..13410
                     /locus_tag="AC162G_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076223.1"
                     /translation="MGETRRWISNSMMRHALQVLEELLAHNGLTAALRLSGLERYIDD
                     LPPDDDQMDIPRGDLNALFAAIVSMYGDQGARGVLRRWGRAFAVRRLKRRIALRVLRL
                     GLRLIPAERSAPYVLARLLHHLDLASEDRPPTMEDSGASFLLELADCLYCPGQKPSQP
                     YGNAVVGLVEGLLRWATGHDFEVTDQSSAGQGAVVLEIRKQPVGRR"
     gene            13416..14768
                     /locus_tag="AC162G_10165"
     CDS             13416..14768
                     /locus_tag="AC162G_10165"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR5076224.1"
                     /translation="MPDSINRIALLALLAFVCLAGALGYWVASGASLTAREDNPRHIF
                     AEQRTLRGSILDRDGEVIVETTGEPGAYTRRTLYPDAAPVTGYYSINYGTSGIEQAFD
                     SMLRGTLGIDPLRADLDALMHIQPIGRDVQVTLDLDVQRVADALLADRTGAIVVLSVP
                     DGEVLALSSHPTFDPNTLDENWDRLRADPASPLLNRATQGEYQPGTALQPVILAKALR
                     QGIVALDDAPESPAAPFPIDSQSLTCRTADDVRTLSDALRSACPAPFADLGAALGSDA
                     LWNVVSTWGLTQTDTSLSGIGSRPTLTQTIPLTDTQALREFAAGQGGLTVSPLQMATM
                     VSTLASRGVLVSPRIISATESVDGAWQGVGRVVPHRVAPSDVVQQVVSAMPQESDVAW
                     HAGTGLSGRSKLHWFVGVTPIDAPRYAAAVLIEDAEGRLASEREAAEIGLKLLTALPR
                     "
     gene            14975..15157
                     /locus_tag="AC162G_10170"
     CDS             14975..15157
                     /locus_tag="AC162G_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076225.1"
                     /translation="MGTGAVPGYGIPESKTGLLLWSQLTECMAKAQYYWMCTASLDGH
                     PHAAPVDGSWLEDQLF"
     gene            complement(15154..>16677)
                     /locus_tag="AC162G_10175"
     CDS             complement(15154..>16677)
                     /locus_tag="AC162G_10175"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5076226.1"
                     /translation="LGQTVLIFPSPDAQSYEDSIEAGRKFIEKWKGHPLVVPAIAPHA
                     HYTCPPEVLRACVDLATEYSVPLHTHVSETALEVEESRKQHGMPVVPWIKKNGLLDTK
                     LIAAHCVHVDPGEIRTLQRAGAGVAHNPSSNLKLASGVAPVVQMLEAGLKLGIGTDGC
                     ASNNDLDMFEEMRLASFIAKGVSGDPLAVPARSTLMLATIGGARALHLDHLTGSLVPG
                     KRADVAVVDLTGLHNTPRFQRDPEAVYSQLVYSCKAGDVRDVWCNGRPLMRSRALLTI
                     DVKAVAARAQEIAAQIDAFLVAREGSVLDKLVAVGGLEQQETYEVQVKVTIEDPQVVM
                     TGLTGPDIVIERSSVRRQYDTYFSFEDPLQGRIRYREDEILDDAGQAKEVRYSLTLIG
                     PAKEREFAHSVLLSRSRFTARAERSRRFYHEYFRPIEEREVQKERRRWHIRYKNTELA
                     VNLDRLTLPERPGWYLEIKSLTWSPRDAETKAELIRELLEQLGLYAQWVTRSEYVDF"
BASE COUNT         3010 a         5455 c         5283 g         2929 t
ORIGIN      
        1 gcgagcggca gcgcgccatc gcccggcaga ttctcaagga gatccgggcg cgcctgcaat
       61 tcatggtcga cgtgggattg gattatctca cgctgagccg cacggcgtcg acgctctcag
      121 gcggcgaggc gcagcgcatc cggctcgcca cgcaaatcgg ctcgcagttg atgggcgtac
      181 tgtacgtgct cgacgagccg tcaatcggcc tgcaccagcg cgaccaggcg cggctgatca
      241 agacgctgca ggcgatgcgc gacctgggca atacgctgct gatcgtcgag cacgaccacg
      301 acacgatgga ggccgccgac tggatcatcg acctcggtcc cggggcgggc gtgcacggcg
      361 gcgaagtgat cttcgaagga accgtcaagc agctcaaagc ggacaggaaa tcgatcacgg
      421 gcgcgtatct gtcgggccga acgaaagtcg aaatgccggc gcagaggcgt gtgggtaacg
      481 gcaaattcct cgtcgtgcgc ggcgcgaccg agaacaatct caagaagatc aacgtgtcga
      541 tcccgctgag caagttcgtg tgtgtgaccg gcgtgtccgg ctcgggcaag tcgacgctcg
      601 tcatcgaggt cctgttcaag cggctggcgc agatgctgca cggcgcgcac gaagcggccg
      661 gcgcgcacat cggcctcaag ggcgtcgagc acatcgacaa aatcatcaac atcgaccaat
      721 cgccgatcgg ccgcacgccg cgctccaacc cggcgactta caccggcttc ttcggcgaca
      781 tccgagacat cttcgccaac ctgcccgagt cgaaggtgcg cggctacggg ccgggccgct
      841 tctcgttcaa cgtcaagggt ggacgctgcg aggcgtgcga gggccagggc caggttcaga
      901 tcgagatgca gttcctgccg gacatctttg tcccgtgcga ggtctgccac ggcgcgcgct
      961 acaatcgcga gacgctgcag gtgcgctata agggcaagaa catcgcggaa gtgttgagca
     1021 tgacggtcga agaggcgctg gcgttcttcg agaacatccc accgctgcgg cgcaagctcg
     1081 agacgctttc gcgcgtcggg ctgggctaca tcacgctcgg ccagccggcg acgacgctat
     1141 ccggcggcga ggcgcagcgc gtgaagttga gccgtgagct gagcaagcgc agcaccgggc
     1201 ggactctgta cgtgctcgac gagccgtcgg tcggtctgca tgccgccgac gtgcacttgt
     1261 tgatcgacgt gctgcagcag ttcgtcgacg cgggcaacac ggtgctgatc atcgagcacc
     1321 atccggacat catcaaagtc gccgaccaca tcatcgacct ggggccagag ggtggcgagg
     1381 ccggggggcg catcgtcgcg gagggaaccc cggaggagat cgcacaagtt cgggagtcgc
     1441 acacggggca gttcttgaag cgcctgctcg gaaacggctc aagaagccga caataacctt
     1501 cccgcagagt atgccacaag agtgctggcc accccgccca ctgcgctcgg tggaccgcca
     1561 aatccacagc aaacgagctg aatggcgcag gacttgacaa tcctgcgcga gcaaccggga
     1621 aagccatcga tcattctgcg taatgctcgg tcacgcaggg gttgttgaca aaccaagtcg
     1681 agcgagtaga atcaggcgca gtatccagcc caggagactg ccgtgtccac ttcgtccagc
     1741 gcaccgaatg cctatcaagt cgcgcgccag cagttcgagc gcgccgccgt ccacctcgaa
     1801 gatataccgc ccggcatcat cgagttcctg cgtcatccca agcgcgaact gacggtcaat
     1861 ttcccggtca aaatggacga tggccgcctg cgcatgttca ccggctaccg ggtgcactac
     1921 aacaacgtgc tcggcccgac gaagggcggc atccgcttcc acccggatgt gacactcgac
     1981 gaagtccggg cgctggcgat gtggatgacg tggaagtgct cgctgatgaa catcccgtac
     2041 ggcggcgcga agggcggcgt cgccgtcgat ccggcgcagt tgtcgccggg cgagctggag
     2101 cggctgacgc ggcgctacgc taccgacatc agcatcctca tgcacccgag caacgacgta
     2161 cctgcgcccg acgtcggcac caacccgcag atcatggcct ggatcatgga cacgtattcg
     2221 atgcacttcg gctacattca aatggccgtc gtcaccggaa agccgatcga ggtcggcggc
     2281 tcgctggggc gcacggacgc gaccggtcgc ggagtgatga tcacggcccg cgaggcgatg
     2341 aatcacctcg gcatcccgat ccccggtgcg cgcgtcgccg tacagggctt cggcaacgtc
     2401 gggtcggtcg ccgcgcacac ggcttacgaa atggactgca aggtgatcgc cgccagcgac
     2461 gtgggcgggg gcatcgtcga taagggcggg ctgcatgtgc catcgctcgt cgagcatgtg
     2521 cgcaagactg gatcggtcgt caactttccc ggcaccgaac cgatcgacaa cgccagtctc
     2581 ctcgaactcg aatgcgaggt gctcatccct gccgcgctgg agaaccagat cacgcaggac
     2641 aacgcagcga atatcgaggc cggaatcgtc gccgaaggcg cgaacgggcc gacgaccaac
     2701 gaagccgatg ccatcctcga cgcgaacggc gtgttcgtca tccccgacat tctctgcaac
     2761 ggcggcggcg tgaccgtctc ctacttcgaa tgggtgcagg atcttcaatc cttcttctgg
     2821 gatgaggagg aaatcgcccg gcggctcgag cgcacgatgg tcaaagcatt ccacgacgtg
     2881 ctgcgcttct cccaggagcg caagatcaac ttgcggacgg cggcgcaggc gttggctatc
     2941 cgccgggtag cgcaggcggc gatgatccga ggcatctatc cctaaccacg aaggcacaaa
     3001 gacaccaaga cgttcaacac ctgcctgcaa ccgacgcaat gccttgcagg aacttcgtgc
     3061 cttggtgtct tagtggtaag aacgaccgtg acgcttactg cacgaatcag aggcagggca
     3121 accgatctcg gcttcgacct gatcggcgtc gcgccggccg ggcgcgcttt gcgtgccgat
     3181 gcttacgtcc aatgggtgga ggccggccgc gcaggcgaga tggagtacat gacgcgcgat
     3241 cctcagcgac gccaggatgc ccggcacttc atgcccgagg cgcgctcggt ggtcgtcgtc
     3301 ggcttgagtc actacactgt caatctaccc gatgagatca agcgtgatcc atcacgcggc
     3361 ctgatcgcac gctacgcctg ggggttagat taccacgaca tagtcacacc gcgcctgcgc
     3421 gagctgttgg ctttcatccg cgctgaagcc aggcgcgaga tacaggccaa ggtgtacgtc
     3481 gataccgggc cggtgctgga gcgcgatttc gccgaacggg ccggactggg cttcaccggc
     3541 aagaacacct gcctgatcca cccgcgcaca ggctcgtggc ttttcctcgg cgagatcatt
     3601 ctggacctgg agctagacta cgacgtaccg gtcacgagca ttggttgtgg ctcgtgcacg
     3661 cgatgcctgt ccgcctgccc gaccgacgcc ttcgtcgctc cctacgtcct cgacgcgcgc
     3721 cgctgcatct cctatctgac gatcgagctg aaaggcaata tcccacgcga gctacgccca
     3781 ccgctgggca atcgcatctt cggctgcgac gaatgtcaag acgtgtgccc ctggccacgg
     3841 cgattcgccc gcccaacctc tgagaaggct ttctacccga ttgacttcga gcgagccgcc
     3901 ccgaaactgc tcgacgtcat cggcctgagt gccgagcagt tccgagagcg attcaggggc
     3961 acacccatcc tgcgcgccaa acgcaagggc ttgctgcgaa acgccgccat cgcgatcggc
     4021 aactggggag acgagactgc tgtgcctgga ctggtcaacg cgctgaatga cccggaaccg
     4081 ctcgtccgcg cgcacgttgc ctgggcactc ggcgaaatca gcacactcgc tgcccggaac
     4141 gcactcgaaa cacaagccaa gattgaaccc gatccttccg ttcagcagga aatcgccgac
     4201 gcgttgattc gctgactcgc cagtttgctt ttttcgctct tatccttcgt cagacgcacg
     4261 ggttgggttg ctattgtccg attcgatgcg cctaatcgaa accagcgtca gagcgagatc
     4321 gcgcattttc atcaacactc cgtacagcgc cgcctggtcg ggcagcgcgc cggtgagaat
     4381 cgtttcactg cgcccggtgc gcgtcagggt cataccgcca agccacgccg accagtggtc
     4441 atcgagatgg ccctcgactc tgatctcgta agtggcggcg ggcagtttca tacaccactc
     4501 ttttgcgcga acctcgccgt aggcgctcgc gcgacgagcg tcgcggccag ctcccaggca
     4561 cgctcctgat tcccgatatc gagcgcgagc gcagatgggc gtttgggctt cgcgtcactc
     4621 tcgaagtaca ttcccgaaac actcgcagcg tcgtccgacg aggcgaggaa gaccgaagac
     4681 cttgacgcag cctccgcgga ggctctgcgc tgaatccacc gcaccagcgg ccaagtaaat
     4741 cgccaggccg gaacggcctg cggggtgagc gcttgcgtgc tcggcgtcca ggccatcccc
     4801 gggttggtcg cgttgacgac gacaccagac ccctccagcc gtcgagccaa cgcgaacgtc
     4861 cagaggaggt tcagcagctt cgcgtgcgcg tgggcctcga tacccacgta acgttctctc
     4921 gcatcgagat cttcgaatgg gtcccgcttc cacatggcgt gggcactgga gaccacgttg
     4981 acgatgcggg caggggcgct ttcgcgcagt agctgcagca ggccgcgggt cagggcaacc
     5041 ggcccgacaa agttcatggc caggctggcc tcgtaaccat ctgccgtctc ctgacgttga
     5101 ccgttcggcg aacccccgac gttattaatc aggccgtcga ggcggtcgag ctccttcgcg
     5161 actcgatccg caaggtcgcc attgccgccg acggtcgaat ggtcgacggc gacgaagtgg
     5221 acctggtcgt gcccggcgcg gcgacgcagt tcgtcgatgg cttcacgtcc gcgtttctcg
     5281 tcgcggccgg ttacgatcac ccgtgcgcct tttgcggcga gggcctgtgc ggtgtgaaag
     5341 ccgattcccg acgtggcccc cgtgatcaaa atggtcttgt ccttcaagtt gcaaccctcc
     5401 ttcgaatgga ttctccggcg cgaggctggt cagcaccgaa gattggagtg ctcggcgaat
     5461 ctgccggcgg cagggcgcat cgcctcctcg cggtcaccag gttactggaa cgggcacgcg
     5521 gtcacagggc cggcttgaac accatcgccc acacggcgga cagcagcagg agcaggttga
     5581 tcgtgctcag ggttgccagc cgctgctgta gcgcgatcac gcgcggatcg ttgggcgcta
     5641 cagtcggcgc gagcgcgctc agctccgccg tggcacggcg gatgagggct acccccagga
     5701 caagcgagcc gaagactccg acaagccccc atacgatcca gagggtgccg aagccgagac
     5761 ctaagaccgc gaccatcacg ataccagtga tgagtgtgat gccagcggcc gggcccaaaa
     5821 tggccctgcc gtagaagtcg ctctggcgcg acaacgccac cagcgccgcc cggtcctgct
     5881 cgcgggcgag ccgcacgttg atgatgctga gggcgacgac gcagccgatc cagacaatga
     5941 acgcggcaat gtgcaggaac tcgaataggt tgtagatggg gaacacagat cctccttgcc
     6001 ttgtcggcgt cgctccccgc aacaccaagc ggggggcgac gccgtgcatg tgctcggctt
     6061 cacccggcgc gcgcgatttt gggggacgat ctcacgcccc ctttttaccg cagggttgag
     6121 ataggcgtca tgtcccaaat gagatattcg attgggatat tgtggtggga tatgcgggag
     6181 ggctacagga gtttcaacgc gcgggcgcgc tccacggctt gcacgcggct atgggtgtcg
     6241 agtttgccat agatattctt gaggtgattg cgaaccgtac ccacggtaat cacgagttca
     6301 tcggcgatct gtgcggtgga cagtccggcc gcaatcagcc tgagcacttc catctcgcgc
     6361 gggctgagtg gctcgatgag cgccggggac tgcgcgtgaa tgggcgggga taccggcgga
     6421 tgtgtcggcg cttccacact tccttgctct gacttggcta acactgccag caatctctcg
     6481 gcatagggcc actgctcacc cgtgacctgc tgcaacaatc ctgccactgg cttgccttcg
     6541 tccacaaaga cgcggacgta gccttccggg ccagccaggg tgagcgcctc gaccaggacg
     6601 gccagggctc gagcctgctc gcctatcgcc ttgagggcga gcgccttgag aacccgcacc
     6661 tcaatgacgc tccccgtccg cgcctgcgcc tccgccacag agccgaggcg ctccagcagg
     6721 ctgagggctc gatcggacat gttccgggcg agcagcaccc gggccagcac gagatactca
     6781 cactcccgag ggtagctgag ttcggcgtcc tcttccagcc cttgcgcctc cacccagcgg
     6841 gcggcttcca tcacgtcgcc ttgggcaagc catagccggg cccgttctgc aggcaccggg
     6901 ttgtgcaggg cgacgacctc caaggccggc atgacctgat aggcctcgtc catcgcctcc
     6961 agggcgccag ccgggtcccc cctggcctgc cggatccagg ccagggtggc caggccggtg
     7021 gctagcgact gggtggaggt gagttgccgg ccgagggaga tgccctccgt cacgtggcgc
     7081 agggcctgct cgagctggtt gcgctcgtag agcacctcgg ccatccccat gtgcgccatg
     7141 gccgcgctca gcaccaccgg agagccggtt tgggcggcga actctaggcc tttctgatag
     7201 gtgcggaggg ctgcgtccag gcggccctgg gcacgctgca cacggcccag gactgagcca
     7261 gcgctcaaca tcaggtgcgg ctcgcccgct acccgaccct cggccacgat gccggcgaag
     7321 gcgcgctcgg cctccgccag gcggccacgc atccagtccg caagcgccag gttccagcga
     7381 accgagatgt gcgggcctcg ctcgtcctcg gtcagatggg tcagggccaa ccgcacgagc
     7441 tcggtcattc gctcggcatc accccgcagc cctaccaggc tagcacgcag gagcgcgata
     7501 gtcgccggga cgttggccag catgctcacc tctctgccga tcgaaggcgt gtagggttcg
     7561 ctgggagtgg tcgcgagcgc ccgctcagcg tcctcgagca gcggctcgac cgcctccagc
     7621 cggccggtgt taaacgccgc gatggcctgt gcgagagcca accgtgggcg ggagcgtacg
     7681 gccgcctggg tgactgccgc gagccaccgc cgcagcgtct ctccttcacc tcgactgagc
     7741 acctcctcga cgtgctgttc gaccagctgg gccgcccacg ccgtgtcgcc cgcctcaagg
     7801 gcatggtgca cggcgtcatc aatcagccca tgctcttcgg accaagtcgc cgcctttcgg
     7861 tgtatctccg ctacgcggcc gggtgctgct tgctgcagcc gaaggcgcaa ggcctccgca
     7921 aaaagatgat gatagcgata ccagcgtcga tcctcatcca gcgggatcag gaagagattc
     7981 gcgcgctcca actcctccaa catggcctgc ccatccgcac gtcccgtgac cgcgtcacac
     8041 agcgatccgc tgaggcgggc caggatggag gtttggagca agaaggcttg tgtggtttcg
     8101 ggctggcgtt gcaggacttc ctcgaccaga taatccagaa tgtaacgctg gctgccggtg
     8161 aacgtcgaga caaaatgacg cttggccgtc tcatcgcgtc ccaggagcga gagggcggcc
     8221 aattgcagcc cggcaatcca accttcggtg cgggcctcga gcgcggcgat gtcatcggcc
     8281 gagagatcca agcccatcac ctgattcaag aacacggcca cttcgtccgg cgtgaaacgc
     8341 aggtcgtcag cccgaaattc ggccatccgg ttgctcgcgc ggagacgggc gaggggtagg
     8401 gggggatccg cgcgactggc gataatcacc tgcgtctcgg gtggcaaatg gtcgagtaga
     8461 aaggtgaggc cttcatggat cgcgggcgtg gtgacgacat gatagtcgtc gagcacgagc
     8521 gcgaagccct cccgggacgc cgccatctca ttcagcagca cggtcaggaa ggattcgagg
     8581 ggaattggct gctgccctgt ctcctggagt atcgcctgta cactttcgcc aaaatcagcc
     8641 tggacagttt ggagtgcagc gatgaagtag gtccagaagc gtgtgggatc gttgtcgccc
     8701 tcgtccagtg agagccaggc gacgcggcgg tggttatgag cgatccagtc gccgagcagg
     8761 gtcgtcttgc cacaaccggc gggtgcggag atcagcagga gcgggcgcgc cagcgcctca
     8821 ttcagtcgct cgatgaggcg cgggcgaggc acgatgcgcg agccggttcg cgcggcgggg
     8881 aggtagagct tggtagcgag gagcaccata gacacagacc tgggtcatcc tcaagcgcca
     8941 taataatccg acggcaagga cgttatttaa tcataccgca cgctcgcgcg caagcgctgg
     9001 tgcgaggacc cagggacgcg agcgcaaccg cccagcaaat ggagcaactc cggtccgcaa
     9061 ctaccgctcg cgcgaaccgt gaagaaagtg gccttcgatg cccgacatga ctaaccacac
     9121 cgagacgtaa accgccgcga tgaccggcgc taaccgctcc agcgccgaga tccggccttt
     9181 ccagatcgac atcccacata cccaatttcg cacggactgg ctaacaccat cgattcgggc
     9241 caaatacccc atcctgtaca tctttgctgc agcgactata ctcggcggaa gcgaacatgg
     9301 aaaggtcctg aagcactgca gagcactgca gaacacacct tgacgcgccc gcgcgtcaac
     9361 ggtacaatcg ctcccacttg gagaagccag gtttctaagc ggtagcgccc tgaagtacca
     9421 tcttcacgcc ttcggctgcc agatgaacga ggccgacgct gcccatccat tgacacccca
     9481 cactcttcct agcagaccca aaccttacgt catggcccat cgaggcaaca gcgccgccgc
     9541 gccggaaaac acgctcgctg ccttccgccg cgcaatcgac gacggggcgg acattatcga
     9601 gaccgacctg cacctaacgc gagatggcgt ctttgtgtgc attcacgacg cgacgctcga
     9661 tcgcacgacg gacggtcacg gagcggtggc cgacctcaca ctggccgaga tcaagcgata
     9721 ttccgccagc tacggcaggc ccgagttcgc cgccgagcgc gtgccggcgc tgggcgagct
     9781 caccgcgatt cttccgccgg acgttgtcct cgcgctggaa ctcaagaccg accgcttcct
     9841 ggagcgcggc gtcgccccac ggctcatcga ccagttgcga gccgacggcg tcttcgatcg
     9901 cgtcatcgtg ctgtcattcg gcctggagcg cattcaggcc gtgcagcgtg ccgcgccgga
     9961 gataccgatc ggctacatca cgctcacgcg cctgacgccg agcgtcccgg cgcagatgat
    10021 cgggccgttc tggccgctgt tggccgtcaa tccgctctac acgtggtggg cgcatcggcg
    10081 cggcatgctc atcgcccctc tcgatccgtg gccgaatggg cgactgtggc tgtatcgact
    10141 cctgcggtgc gacgctatcc tcacaaacga tcccgccggc actatgaaga agctgggacg
    10201 cactctcaaa tcctagcgat cggcgcatcc tcattcgtgg gtttgtgtcc ggttcgagat
    10261 atcgctatac tcggcaggtc tatgtttgcc cgtggtacaa tagagccggc tcccgcaacg
    10321 ccggaagcgc aggacaccgg gggatcccca tgcccaaact cgaatccgtt caactcgcga
    10381 tcatcggcgg aagcggcctt tacaaaatgg agtcactggc cggcatcaag gaatatcgca
    10441 tcgagacgcc gttcggcgat cccagcgatg cgatcgtcgt cggcactctg agcggccggc
    10501 gcgtggcttt cttgccccgc cacgctcgcg gccatcgcgt gctgccctct gagatcaacg
    10561 cgcgagccaa catcttcgcg ctcaaaatga tgggggtgac gcacatcgtc tccgtcagcg
    10621 cgtgcggcag cctgcgcgag cacatccacc cacggcacgt cgtcgtgccc gatcagttgt
    10681 tcgatcgaac gcggggccgc cccttgacat tcttcggcaa cgggatcgtc gtccatatcg
    10741 gactggccga tccgttctgc ccgaacctga gcgcgctgat ggcccaggct gttgaagcgt
    10801 caggtggcac tgttcaccga ggcggcacgt tcgtgaccgt cgaaggcccg cgcttcagca
    10861 cgaaggcaga gagccgtgcc ttccggcaac tcggcttcga cattatcggc atgacggcga
    10921 tacccgaagc caatctcgcg cgggaggccg agatcgccta tgcggccatg gcacacgtca
    10981 ccgatttcga cgtgtggcac gcgtcggaag aagccgtcac cgtcgaaatg gtgatccgca
    11041 cgctgaacga aaacgtggcg ctcgccaagg aagccatcca gcgcatcgcg gctgccgatc
    11101 tgccgccgag cgacgcacat tcggcgctat cttcggccat catcactccg gcgcatctca
    11161 tccccgacaa agtcaagcgg gcactggcgc ccatcgtcgg caaatacctc ccggccaggc
    11221 ccaggtcgca ggcgcccaga gcacagcgaa ggggaaagag ccggcaacgc aaagcgtgaa
    11281 gcacgccggc tgacccgtcg tccaccctga gcgaagccga agcggaagtc gaaggacgcc
    11341 ctgtatgcag gacacgctct ccttccgacg caatttgctg gcccggccac gcgcgcgcga
    11401 gcgggcattg ctgctcgtcg gcggcgcctt tgtcgtcgcc gggttggtgc agttgagcat
    11461 cgtcgatcca ctctcgatgg gtgcgtcttt atacctcgcc gcatccgttg tctgggcgct
    11521 cgtcttcgtg gtagcccacc tcgcgctcga ccgttatttg cccgggcgag atcctctgat
    11581 actgccgatc gctgccctgc tgaccgggtg gggactgatc gaagtggcgc gcctgtcgcg
    11641 cacggatgcc ctccggcaac tgatgtgggt cgtcgtcagc gtcggcgtga tgctgatcgt
    11701 cgcggccgcg ccgcgggagt tgagatggct gcggcgctat cgctacacct ggctgctcgg
    11761 tggactcgtg ctcctcgcgg cgacactcgt cttcggcgtc aacccgtcgg gaatcgggca
    11821 agagctgtgg ctcggcttcg gcggcatctt ctttcagcca tccgaggtcg tcaagctgct
    11881 gatggtcgta ttcctcgcat cgtatctggc cgaaaagcgc gacttgcagt tggtcatgcg
    11941 catacagtct cgcagcttga ccgtggctta cctggcgccg atgctggcga tgtggggcgt
    12001 ggctatgacg atcctgctca tccagcaaga cctcggcgcg gcgatgctgc tgttcctggc
    12061 tttcctggtc atgctgtatc tggcgagcgg caaatggcag tacctgttga tcggaatggt
    12121 cctgctagcc tgtgccgtgc tcatcggcta cgcgttgatc ggtcgcgtca ccgaacgggt
    12181 gaacacctgg ctcgatccgt ggtcgcgcgc tgaagtcgag tcgtatcagg ttgtgcaatc
    12241 gctgatcgga atcggggcgg gggggctttt cggacgcggg ctggggcaag gcgatccggt
    12301 cgtgccagcc gcgcacacgg acatgcccct ggccgtcatc ggagaggagt tcgggctgct
    12361 cggtgcgatc gggctggtgg cgtgctacgc cctcttgacg ctgcgcggct tgcgcgccgc
    12421 agcgctgtcg cgcaccgtgt tcggctgctt gctggcggcc gggctggccg cactgctcgg
    12481 catgcaggca tggatcatca tggccggcaa catcaatctg atcccgctga ccggcgtcac
    12541 gctacctttt gtatcatatg gcggcagctc tctggtcgtc agcttcgtga tggtcgggct
    12601 gctcttgcac gtctcggcaa acgccgacta agagcgtatc cgagaattgg agtgcagact
    12661 tcagtctgca tggctaagcg ggctaaagcc ccccactcca aggttttcgg atagactcta
    12721 agccgaagag cctgtcccgc gaagtccccg accgaaggga gcgggaagcg cagtcgaagg
    12781 aacgcgcgaa tgggcgaaac ccgccgctgg atttcgaatt cgatgatgcg ccatgcgctg
    12841 caagtcctgg aggagctgct ggctcacaac ggcctcacag cggctctgcg cctatcggga
    12901 ctggagcggt acatcgacga tctgccgccg gacgacgacc agatggacat accgcgcggc
    12961 gacctcaacg ccttgttcgc ggccatcgtc agcatgtacg gcgaccaggg cgcgcgcggc
    13021 gtgctgcgac gctggggacg agccttcgcc gttcgccgcc tcaagcggcg catcgcgctc
    13081 cgcgtgctgc ggctcggcct gcggctgatc cccgcggaac ggtccgcgcc ttacgttctg
    13141 gcgcgactgc tgcaccatct cgacctggcc agcgaggatc ggcctcccac gatggaagac
    13201 agcggcgctt cattcttgct cgagctcgcc gactgcctgt actgccccgg tcagaaaccg
    13261 agccagccgt acggcaacgc cgtcgtgggc ctggtcgaag ggctgctgcg ttgggcgacc
    13321 gggcacgatt tcgaggtcac cgaccagtcg agcgccggcc agggcgcggt cgtgctcgag
    13381 atacgcaagc agcccgtagg ccggcgctga gggacatgcc tgattccatc aaccgcatcg
    13441 cgctgctcgc gctgcttgcg tttgtatgcc tggccggcgc gctcggctac tgggtggcga
    13501 gcggcgcgag cctgaccgca cgcgaagaca acccacgcca cattttcgcc gagcagcgca
    13561 cgctgcgcgg ctcgatcctc gaccgggacg gcgaagtgat cgtcgagacg acgggagagc
    13621 ctggcgccta cacccggcgc acgctctacc ctgacgccgc gcccgtcacc ggatactatt
    13681 cgattaacta cggcacgtct ggcatcgagc aggcattcga ttccatgctg cgaggcacgc
    13741 tcgggatcga tccgctccgc gcggatctcg acgctttgat gcatatccaa ccgatcggcc
    13801 gagacgtgca ggtcacgctc gacctcgacg tgcagcgtgt cgccgacgcg ctcctcgccg
    13861 atcggaccgg cgctatcgtc gtgctgtcgg ttcctgacgg cgaggtgctg gcgctcagca
    13921 gccacccgac gttcgatccc aacacgctcg acgagaactg ggacaggctg cgggcggacc
    13981 cggcgtcgcc tctgctgaat cgcgccacac agggtgagta tcagcccggc acagcgctgc
    14041 agcccgtcat tctggccaaa gcgctccgac agggtattgt tgcgcttgac gacgcgccgg
    14101 aatcgccggc agcgccattc ccaatcgact cgcagtcgct gacttgtcga acagccgacg
    14161 atgtgcgcac gctttccgat gcgcttcgct ccgcctgccc ggcgcccttc gccgatctcg
    14221 gcgcggcgct cggaagcgat gcgttgtgga acgtcgtgtc gacgtggggg ctcacgcaga
    14281 ccgatacgag cctgtcgggt attggaagcc gccctaccct tacacagacg atcccgctga
    14341 cggacaccca ggcgctgcgc gagttcgccg ccggccaggg ggggcttacc gtctcgccgc
    14401 tgcaaatggc tacgatggtg tccacgcttg cctcacgcgg cgtcctggtg tcgccgcgca
    14461 tcatatcggc gaccgaatcg gtcgatggtg cctggcaagg cgtcggccgg gttgtgccgc
    14521 accgcgtcgc gccgtccgac gtcgtgcagc aggtcgtatc ggccatgccg caggagagcg
    14581 acgtcgcctg gcacgccgga accggcctga gcggacggtc gaagctgcac tggttcgtcg
    14641 gggtcacgcc gatcgacgca cctcgatacg cggccgccgt cctcatcgag gatgcagagg
    14701 gcaggctcgc gtcggaacga gaagccgccg agatcggtct caagctgctc accgccctgc
    14761 cccgttgagg ttgatacctc acacttgact tgggcgacag tcgattccga ggtacactgt
    14821 catgcagacc tcccgtgtcg atatccggag gcccaccggc ggacgcgggt gactgtgtcc
    14881 ttgttaagac ttcggttttg aggcggtcta tgccattgac acactcgaca tcttgcgagg
    14941 ccagttacca cgacgcgcac agcatggtca tcgagtgggc actggcgcag tgccaggcta
    15001 cggcatcccc gagagcaaga cggggctgct tctctggtct caattgaccg agtgcatggc
    15061 gaaggcccag tactattgga tgtgcaccgc cagcctcgat ggtcacccgc acgccgcccc
    15121 ggtcgatggc tcgtggcttg aggaccaact gttttagaag tccacgtatt cgcttcgcgt
    15181 cacccactgc gcgtacagcc ccagttgctc gagcagctcg cggatcaatt cggccttcgt
    15241 ctcggcatcg cgcggcgacc aggtaaggct cttgatctcc aggtaccagc cgggtcgctc
    15301 gggcagcgtc aggcgatcca ggttgacggc cagctcggtg ttcttgtacc gaatgtgcca
    15361 gcgccgccgc tccttctgta cttcgcgttc ctcgatcggc cggaaatact cgtggtagaa
    15421 gcgccgcgag cgctcggcgc gcgccgtgaa gcgtgagcgc gacagcagca ccgagtgcgc
    15481 gaactcgcgt tctttcgccg ggccgatcag cgtcagcgag tagcgaacct ccttggcctg
    15541 gccggcgtcg tccagaattt cgtcttcgcg atagcgaatg cgcccctgca gcggatcttc
    15601 aaagctgaaa tacgtgtcgt actggcgacg cacggacgat cgctcgatca cgatatcggg
    15661 accggtcagg ccggtcatca ccacttgcgg gtcttcgatc gtgaccttca cctgaacttc
    15721 gtaagtctct tgctgctcca ggccgccgac ggcgaccaac ttgtcgagga cgctgccttc
    15781 gcgcgcgacg aggaacgcat cgatctgcgc agctatctct tgagcgcgcg ccgccaccgc
    15841 ctttacgtcg atcgtcagca gcgcgcggct gcgcatgagc ggcctgccat tgcaccacac
    15901 gtcgcgcacg tcgccggcct tgcacgaata gacgagttgg ctgtagaccg cttcgggatc
    15961 gcgttggaag cgcggcgtgt tgtgcagccc ggtcagatcg acgacggcga cgtcggcgcg
    16021 cttgcccggg acgagcgagc cggtgagatg atcgaggtgc agcgcacgcg cgccgccgat
    16081 ggtcgccagc atgagcgtcg agcgcgccgg cacggccagc gggtcgccgc tcacgccctt
    16141 ggcgatgaac gaggccaacc gcatctcctc gaacatatcc aggtcgttgt tgctggcgca
    16201 gccgtccgtg ccgatgccga gcttgaggcc ggcctccaac atttgcacaa ccggcgccac
    16261 gccgctggcc agcttgaggt tgctggaggg attgtgcgcg acgcccgcgc ccgcacgctg
    16321 aagcgttcgt atttcgcccg gatcgacgtg gacgcaatgc gccgcaatca gcttcgtatc
    16381 cagcaggccg ttcttcttga tccagggcac gaccggcatg ccgtgctgct tgcgcgactc
    16441 ttccacctcg agcgccgtct ccgagacgtg cgtgtgcagg ggcacactgt actcggtcgc
    16501 cagatcaacg caggcgcgca gcacctcggg cgggcaggta tagtgcgcgt gaggcgcgat
    16561 ggcaggcacg acgagcgggt gccccttcca cttctcgatg aacttgcgtc cggcctcgat
    16621 cgaatcttcg taggactgcg cgtccggcga cggaaagatc aacaccgtct ggccgag
//
LOCUS       DBNHXB010000247         4136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4086442, whole genome shotgun sequence.
ACCESSION   DBNHXB010000247 DBNHXB010000000
VERSION     DBNHXB010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4086442"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="AC162G_10180"
     CDS             <1..1130
                     /locus_tag="AC162G_10180"
                     /inference="COORDINATES: protein motif:HMM:NF021379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HIR5076227.1"
                     /translation="GVAVALYLAHPAMWNALLFDFHPVTLAAAFLMFALWMLIQKKRT
                     ACLIFALLAMACKEHIGLIVAMFGVYAVLFQKSERRLGAVLIAMGLAGSILAIGVVNP
                     AYQPSGASYYLNRYSKVGSSIGEILLAPLTKPDVVWATLTGPKRISYYGDLLLPLGGL
                     PLLGPAFILPVLPDIALNTFSAFAPARTLDAHYAVMIAPFLILAAIWGADRLAHWLST
                     RFGDPQRYDTRASGLRVFLESIAQRRAILVLASAWLIVSMAAYHLHNYGTFLPLSGRY
                     VSTYVSEPRDAVGLRLATRIPAAAVVSAQFNLVPHISQRRRAHIFPRIDDAEYVFLDT
                     HGVIEPFQTQAEYQSAVEALRNDPAFEVAAEEEGFILMSRR"
     gene            1408..3414
                     /locus_tag="AC162G_10185"
     CDS             1408..3414
                     /locus_tag="AC162G_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076228.1"
                     /translation="MATLTKFVASQPATHRPPAARQRSRYWQDFFILCGCVAFVMSLY
                     GISLTYPFVWHEADDLSRTLRYSPLEYFTGMSSYEYYRPLMFLAWRVLLNAWGPDSAP
                     VFHALSIYLHLLNAVLLFALARAITGSRLVGWAAAFLLVAYPFSYQAITWSTAHFHPA
                     VLLLVLACVVTYSRARLKTASWPTYALAAAFLVAGILVHETAFAAAPILVLIESYLVV
                     NRRVPRWSRWPLLYVVVTLAVLALYATADKSPPVEQTFQLLSGLYLLQGLIYPVAMLL
                     QRACAWVGCNSVALLLPAVAVTLIALLLVCRTRHALTLGLLGLAWFLLGAAPVWAGRD
                     YVYIEYAPRLLYFAGAGAALALAAVADVPGEMTSPARPSIGLALRLGIIAWIVLQSSL
                     FVAARQPLYAEGFRVVEQENQATFSPRDGMALFINTIDLQDYAQRDFPLGWFGVLVAP
                     WHNRLGAVPHLRSEKAEWIIEPGQAQYLQDRYPRKLEFHGRTVTPELLRGLLAQASHV
                     YQLRALLPMPNLHLFQIAEIERGEGSSSSAWAEWPNGVRLASASIEYEAGIPVLNLDW
                     IINGPVDATQTVFVHVRDTAGNIVTQADGDPIAGLAPFSEWAVGDRIRERRPLDLPVH
                     LPAGAYTVAVGLYDRGSLQRTAPAQANGADVVDGAVVIGTFRRE"
     gene            3479..>4136
                     /locus_tag="AC162G_10190"
     CDS             3479..>4136
                     /locus_tag="AC162G_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076229.1"
                     /translation="MRNIARHQHFLLVLLVFLAFRLLMPFVFRSGSYFVEQAPDIGDY
                     LRWGTLSDSGFYPYLDYWSEYPPLFPWSMVVLYRISTLLPAWPFDQRLWFAIVTQTVT
                     MLFDAGSLVMVYAIAQRLGSRARAVRTAALFAGAFITAYAASSWYEPVPLFFLLAALY
                     LALRDRYVGSAAAAGVGIMLKIFPIVVVPIALRRTTTPRQVAGYLLALAVTIGAILLP
                     F"
BASE COUNT          647 a         1362 c         1241 g          886 t
ORIGIN      
        1 cgggcgtggc cgtggcgctg tacctggcgc atccggcaat gtggaacgca ctgctcttcg
       61 atttccatcc ggtcacgctg gcggcggctt ttctgatgtt cgcgctgtgg atgctgattc
      121 aaaagaaacg cactgcttgc ctgattttcg cgctgctggc gatggcctgc aaggagcaca
      181 taggcctgat agtggcgatg ttcggcgtgt acgctgtgct gttccagaaa tcggagcgac
      241 gactgggcgc ggtgttgatc gccatgggac tggccggatc gattctcgct atcggcgtgg
      301 tcaacccggc ctatcagccg agcggggcgt cgtactatct gaatcgatac agcaaggttg
      361 gcagcagtat cggcgagatc ctgctggcac cgctgaccaa gccggatgtc gtctgggcga
      421 cgctcacagg tccaaagcgc atctcgtact acggcgacct cctgcttcct ctaggaggac
      481 tgccgctctt ggggccggcg ttcatcctgc ccgtcctgcc cgacattgcg ctcaacactt
      541 tttcggcctt cgcgccggcc cgcacgcttg acgcgcacta tgccgtgatg atcgcaccgt
      601 ttctgattct ggctgccatc tggggggccg atcggctggc gcactggctc agcacacgct
      661 tcggcgaccc gcagcgctac gacacacgag cttcgggcct tcgtgtcttt ctggaaagca
      721 tcgctcaacg ccgagccatc ctggtccttg ccagcgcgtg gctgatcgtc agcatggctg
      781 cttaccatct ccacaactac gggaccttcc tgccgctgtc tgggcgctac gtctcgactt
      841 atgtttctga gccgcgtgac gcagtcggtc tgcgcctggc gacaaggata cctgccgctg
      901 cggtcgtgtc agcccagttc aacctggtgc cgcacatcag ccagcggcgg cgcgcgcaca
      961 tctttccccg catcgacgac gccgagtatg tgttcctcga cacgcatggt gtgatcgagc
     1021 cgttccaaac gcaggccgag taccaatcgg ctgtcgaggc gcttcgtaac gatccggcgt
     1081 ttgaagtcgc ggccgaggaa gaagggttca tcctgatgag tcgcagatag cgtaccgcgc
     1141 atcgcttatg gcttatcgct tatcgcctcg cgttttaggt cacgacagga tcccgctctg
     1201 tgggtcgatt tcggcatagt ggtagttcac cgattcgccc aacacaaatg tgttacaatc
     1261 gccaccagct ccgacatctg cccgtttctc atgcggcaac agcgcatgca ccagcttgcc
     1321 acagcgcctg cgaggatggc gcgtgtgcga ccggaatggg tcgtcctggg caccctctat
     1381 tcaggcatca ataggcgcac cgcacgcatg gcaaccctca cgaaatttgt cgccagccag
     1441 ccggcgacgc atagaccgcc cgcggcgcgc cagcggtcgc gctactggca agacttcttt
     1501 atcctgtgcg ggtgcgtcgc gttcgtcatg agcctgtatg gcatcagcct gacctatcca
     1561 ttcgtctggc atgaggctga cgacctgtcg cgcacgcttc gttactcacc gctcgaatac
     1621 ttcaccggta tgtcgagcta cgaatattat cggccgctga tgttcctggc gtggcgcgtg
     1681 ctcctcaacg catgggggcc cgactctgcg ccggttttcc atgcgctctc gatctacctg
     1741 cacctgctca atgccgtgct gcttttcgcg ctcgcgcgcg cgatcacggg cagccggctc
     1801 gtcggctggg ccgctgcctt tctgctcgtc gcctacccat tcagctacca ggccatcacc
     1861 tggtcgacgg cgcacttcca cccagccgtt ctcttgctcg tgctggcctg cgtcgtgacg
     1921 tactcacggg cgcgactgaa gacagcaagc tggccgacct acgcgctcgc cgccgcgttt
     1981 ctggtggcag gcattctggt gcacgaaaca gccttcgcgg cggcgccgat tctggtactc
     2041 atcgagtcgt acctggtcgt caaccggcga gtgccgcgtt ggagtcgctg gcctctactg
     2101 tacgtggttg tcacgctggc agtactggca ctgtacgcga ccgccgacaa atcgccgccg
     2161 gttgagcaga catttcaact cctgtccggt ctgtatctgc tccagggcct gatctatccg
     2221 gtcgccatgc ttctgcagcg tgcgtgtgcc tgggtgggtt gcaacagcgt cgcgctgctg
     2281 ctgccggcgg tcgccgtgac gctcatcgcg ttgctcctgg tctgccgaac gcgccacgcg
     2341 ctgacgctcg ggctgttggg cctggcctgg ttcctgcttg gcgcagcgcc tgtgtgggcg
     2401 ggccgcgact acgtgtacat cgaatacgcg ccgcggctcc tctatttcgc cggggcaggc
     2461 gcggcgctgg ccttggcagc agtcgccgac gtaccgggag agatgacctc gcccgcccgc
     2521 ccatcaatcg gcctggccct ccgtctcggc atcatcgcgt ggatcgtcct ccagagcagt
     2581 cttttcgtcg ccgcgcggca gccactgtac gccgagggct tccgcgtcgt cgagcaagag
     2641 aaccaggcca ccttctcacc gcgcgacgga atggcgctgt tcattaacac catcgatctt
     2701 caggattacg cacagcgcga ttttccgctt ggctggttcg gcgtgctcgt cgcaccatgg
     2761 cacaatcggc tcggcgctgt gccccatctg cgatccgaga aggccgaatg gattatcgag
     2821 ccagggcagg cacagtatct gcaggatcgt tatccgcgca agcttgagtt ccacggccgc
     2881 acggtcacgc cggaattgct gcgcggtctg ctcgcccagg cgagccatgt ctatcaactc
     2941 cgggcgctgc tgccaatgcc gaatctgcac ctgttccaga tcgcagagat cgaacgcggt
     3001 gaagggtcgt catcgtcggc atgggcagaa tggccaaacg gtgtccgcct ggcatcggca
     3061 tcgatcgaat acgaagccgg catacccgta ctcaacctcg actggattat caacggaccg
     3121 gtcgacgcga cccaaaccgt atttgtccac gtccgagaca ctgccggcaa catcgtcacg
     3181 caggcagatg gcgatccgat cgccggattg gctcccttca gcgagtgggc ggtcggcgat
     3241 cggatccgcg aacgccggcc gctggacctg cctgttcacc ttcctgccgg cgcgtatacg
     3301 gtcgctgtcg gcctgtacga tcgcggctcg ctccagcgca cagcgcccgc gcaggcaaac
     3361 ggcgccgacg tggtcgacgg cgccgtcgtc atcggcacat tcaggcgaga atgagggcgc
     3421 atcgcttatg gccgcacctg tacccacatc gagcgaggcc cgccttagag cgcgtgtcat
     3481 gcgcaacata gcacgccacc agcactttct acttgtcctg ctcgtcttcc tcgccttccg
     3541 gctgctgatg ccattcgtgt tccgcagcgg atcgtatttc gtcgagcagg cgcccgatat
     3601 cggcgactac ctgcgctggg gcacactgtc cgacagcggc ttctatccat acctcgatta
     3661 ctggtcggag tatccgccac tgttcccgtg gtcgatggtt gtcctgtatc ggatcagcac
     3721 gctgctgccc gcctggccgt ttgaccagcg cctgtggttc gcgattgtca cccagacggt
     3781 gacgatgctc ttcgacgcgg gcagcctggt catggtgtac gccatcgcgc agcggttggg
     3841 cagccgagcg cgagctgtgc gcacggcagc gctgttcgct ggcgcgttca tcacggccta
     3901 cgccgcgtcg agctggtacg aacctgtgcc gctgttcttc ttgcttgcgg cgctgtacct
     3961 tgcgcttcgc gatcgctatg tgggaagcgc ggcggctgcc ggtgtgggca tcatgctcaa
     4021 gattttcccg attgtcgtcg tgcccattgc actgcggcgc acgacgaccc ctcggcaggt
     4081 ggctggctat ctgctcgcgc tcgccgtgac catcggggca atcctcttgc cattcc
//
LOCUS       DBNHXB010000248         8365 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_898749, whole genome shotgun sequence.
ACCESSION   DBNHXB010000248 DBNHXB010000000
VERSION     DBNHXB010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_898749"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="AC162G_10195"
     CDS             <1..794
                     /locus_tag="AC162G_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076230.1"
                     /translation="DREGILIGKSLANSLGAGVGQKVSLTLVNSDGRPDQGIFTIRGL
                     FSTGIPSYDESAILVPLSKAQAFTGVSDRASAIVILLNQQNDVDKVAAALQSPALAIR
                     TWSDLNQVLLQALQAGMGFYVIMDLIVMLIVAVIIANTLLMSVFERVREMGILAALGM
                     KGRHIMEMFLLEAAILSLAGIVVGIVLGLAGVAYLATTGIPIAEKTAALAGSSVALGT
                     TMYGRFVPDTFAALSIATLVVVLLAALYPARYAARLEPIKALHEL"
     gene            830..1516
                     /locus_tag="AC162G_10200"
     CDS             830..1516
                     /locus_tag="AC162G_10200"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076231.1"
                     /translation="MSIIELENATKIYTLGEVETRALDGVSLSIEKGEFTAVVGPSGS
                     GKTTLLQLIGCLDRPDSGTVKINGKDVTRFSANQRADLRREEIGFIFQFFALVPVLNA
                     YENIELPLLLSGVNAQERRERVMELLKAVGMTDRAKHRPDQMSGGEQQRVAIARALAP
                     RPSMVLADEPTANLDTDNGKQAMEIMQRLNEQTGTAFIFATHDPRVVAFARRVVKLRD
                     GRIVDNGANP"
     gene            1642..3246
                     /locus_tag="AC162G_10205"
     CDS             1642..3246
                     /locus_tag="AC162G_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076232.1"
                     /translation="MAYTPPAQIRVNAQPYLNLRREPVVRPGNVIGRLADGSTWPVVG
                     VAREDYDEKSDVLWVHVESTDASTGKKTLGYCSSAFVNPKPDPLPRAQRVLPEGGLNL
                     RSSPVLDPGVNNKIVTMQTGSTVPVVGGAWENFDPKSGKWWYVVEYQGRRGYAYSRYL
                     GEPTGVQPGPGPTPPRPTPPEPTPPRPAPGRPSPVWKFGVCLAGVGNADQNVWQMPTF
                     PDAIKSARVEAVKIVPLNDLEQMNRVYEWFRSQGIAFVMVRLTWKPDPAWANLPAAAR
                     ITAAVNSFVQTVRGQLEFAYANGVRYFEVHNEPNIPANPTDPVGDGLGTAWMGPEEFA
                     NWFGRVVDDLRKIRPNAYYGFPGLAPRGSQFADFGGGPILVKGQKLLWNTDQWLTVCK
                     PVVLAKADWVGAHCYWQFDGGGLFGIENPDSGGMYWRRYKARFPGKLLFITEFSNNGG
                     AIPYAEKGRQYAKYISLLRREKSIGAAFGYAIFWASDPNREGWVFINEAGKWRVTEIP
                     GAMGAALAANPTTANPVVVSSPAVWA"
     gene            3850..4695
                     /locus_tag="AC162G_10210"
     CDS             3850..4695
                     /locus_tag="AC162G_10210"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR5076233.1"
                     /translation="MLAMMQVGDDRPRQDNGTCSLRLATTEINLPILICLLGNFYVRK
                     RGEPVRVSNGGKAEALLSALALQGDYCVSRDGLLGILWPNTHSELAIQSLNSLVYTLH
                     KRLGDAIGGAAPVLHADGYYLLNVEAGVGVDVACFDALAQAGDREADAGNRSAAAAAY
                     DRALRLYRGDVCAGNDLHAVLERERLRARYLALLTHLADYYFSEGNYAASLDYAQRLL
                     DKDPCREDAHRLVMRSYVRRGERAQALRQYRLCQEILRAEFDAVPEPATTALFDAVRL
                     HPDSV"
     gene            4890..5024
                     /locus_tag="AC162G_10215"
     CDS             4890..5024
                     /locus_tag="AC162G_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076234.1"
                     /translation="MVIDRRERLVHGEVVDVDGKAQGRFDGWREMIHTVRDYLTNQEC
                     "
     gene            5312..>8365
                     /locus_tag="AC162G_10220"
     CDS             5312..>8365
                     /locus_tag="AC162G_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5076235.1"
                     /translation="MIRGIVGWAVQFRFLVLVIAVALIAIGVGQLRNMPVDVLPEFSP
                     PYVEIQTEALGLSAEEVEQMITVPMEQDLMAGVAWLDVIHSESVPGLSSVVIYFEPGT
                     DLFRARQMVSERLAQAAVGIPHVSKPPTMIQPLSSASRFMIVGLSSKDLSLIEMSVLA
                     RWTIAPRLLGVPGVSNVAIWGMRDQQLQVLVDPEQLQAQGVSLQQVVETTGNALWVSP
                     LTYLEASSPGTGGFIDTPNQRLGVWHVLPISSPEDLAQVPVEDAAGLSLGDVAEVVED
                     HQPLIGDAIINDGPSLLLVVEKLPGVNTLEVTRGVEEALAVLQPGMTGIEFDATLFRP
                     ATYIEMAIANLSNALVVGAVLVVVALFAFLWNWRVALISAVVIPLSLMASLLVLYGRG
                     ATLNAIVLAGLVVALGTIIDDAIVDVDNIARRLRQLRKEGSDKSPVLIILRTSAEMRR
                     SILFALVITVLAVLPVFFMEGASGALFQSLVTSYVLAVLVSMVVALLVTPALSMIFLA
                     NAPLERRQSPLVGWLQRGYDRFLARILHNARLAYVAVAVIVLAGVAALPFLEQQSLLP
                     TFKEPYLSIQLEGAPGTSRPEMDRIVARVSQELRTIPGVSNVGAHVGRAVFGDQVVGI
                     NSAQLWVSIDPAADYDATVAAVQETVDGYAGLGGEVSTYLQQTLSQPRTSAGDGITVR
                     VFGEDLDVLRSEADKVRQALVGIAGVVDPHVVLPVEEATLEIEVDLDSAQTHGIKPGD
                     VRRAAAILLSGLQVGSLFEEQKVFDVVVWGTPETRQSLTTVRDLLIDTPGGDQVRLGD
                     VADVRLTSSPIVIRREAVSPYLDVVFNVGGRDPLAVTRDVETALQNVPFSLEYHAELQ
                     HDYEASQAANQRTLLTGAVAVIGIFLLLQAAYGSWRLAAVAILTLPMALAGGVLAALL
                     AGGLSLGSLFGLLMVLGIAVRNGIVMTNHFQYLEQREDQAFGPELVLRGARERLAPIV
                     MTALATGLALAPFVLFGAVPGLEIVRPMAIVILGGLVTSTLLDLFV"
BASE COUNT         1494 a         2490 c         2707 g         1674 t
ORIGIN      
        1 acgatcggga aggcattctg atcggcaaga gcctggccaa tagtctgggg gcgggcgtgg
       61 gacagaaggt gagcctgacg ctcgtcaatt ctgatggccg acccgatcaa gggatcttta
      121 ccatccgagg cctgttctcg accggcattc ccagctacga cgagagcgcg atcctggtgc
      181 cgctctccaa agcacaggcg ttcacgggtg tctccgatcg ggccagcgcc atcgtcattc
      241 tgcttaacca acagaatgac gtcgataaag tcgcggcggc tctgcaaagc cccgcactgg
      301 ccatccgcac ctggtcggat ttgaatcaag tgctcttgca ggcccttcag gcaggcatgg
      361 gtttctacgt gataatggac ctcatcgtga tgttgattgt ggcggtcatc atcgccaaca
      421 cgctgctcat gtccgtcttt gagcgcgtcc gcgaaatggg cattctggcg gcgctgggca
      481 tgaaggggcg gcacatcatg gagatgttcc tgctggaggc ggccatcttg agcctggccg
      541 gcatcgtcgt cgggattgtg cttggcctgg ccggggtcgc gtacctggcg acaaccggca
      601 tccccattgc cgaaaagacg gcggcattgg caggcagcag cgtggcactg ggcactacca
      661 tgtatggccg ctttgtcccc gacacctttg ctgcgctgtc gatcgccaca ctggtggtgg
      721 tcttgttggc cgcactctat cccgcgcggt acgcggcccg gctggagccg atcaaagcgt
      781 tgcatgaact ctaatcagaa tgacactgta ccaggcaagg agcctttcca tgtccatcat
      841 cgaactggag aacgccacca agatttacac tctgggtgaa gtcgaaactc gcgcgctgga
      901 cggggtgagt ctgagtattg aaaaaggtga attcacggcc gtggtgggtc catcgggttc
      961 aggtaagacc actctcttgc agttgatagg ctgtctcgat cggcccgaca gcggcacagt
     1021 gaagatcaac gggaaggatg tgacgcgctt cagcgccaat caacgcgccg atctgcgccg
     1081 ggaggaaatc ggtttcattt tccaattctt cgcgctggtg cccgtcctca acgcgtacga
     1141 aaacatagaa ctgcctctgt tgttgagcgg cgtcaatgcc caggaacgac gcgagcgcgt
     1201 catggagttg ctgaaagccg tcggtatgac tgaccgcgcg aagcatcgtc ccgatcagat
     1261 gagcggcggt gagcagcagc gtgtagccat tgcgcgcgcc ctcgcgccac ggccctcgat
     1321 ggtgctggcc gatgagccga cagctaacct ggacaccgac aacggcaagc aagcgatgga
     1381 gatcatgcag cgcctcaacg aacaaaccgg cacggcgttc atttttgcga cccatgatcc
     1441 gcgcgttgtc gcgtttgcgc ggcgagtggt caagctacgc gatgggcgca tcgtcgacaa
     1501 cggcgcaaat ccgtgacgac ctacccatga tgcgcaaacc cgggtacgaa tgactcccaa
     1561 ttgtacacat tgactttcgc cgtctctggg ctaaaatgag agtgtcggct tgccgcatcc
     1621 tttccgagtg gagaaagaga catggcctat actccgccag cccagattcg ggttaatgct
     1681 cagccgtacc ttaacctgcg ccgcgagcct gtcgtcaggc ccggcaacgt catcggccgc
     1741 ctggccgacg gctcgacctg gccggtcgtc ggcgtggctc gcgaggacta cgacgagaaa
     1801 tcggatgtgc tgtgggttca tgtcgaatcc accgacgcgt cgaccggcaa gaaaacgctc
     1861 ggctattgca gcagcgcctt cgtcaacccc aagcccgacc cgctgccgcg cgcgcagcgg
     1921 gtgctgcccg aggggggcct caacctgcgc tcatcgccgg tgctcgaccc gggggtcaac
     1981 aacaagatcg tcacgatgca gaccggatcg accgtgcccg tggtcggcgg cgcatgggag
     2041 aacttcgacc ccaagagcgg caagtggtgg tacgtcgtcg agtatcaagg gcgacgcggc
     2101 tacgcctact ctcgctatct gggcgagccg acaggcgtgc agcctggtcc gggcccgacg
     2161 ccgcctcggc caacaccacc cgagccaacg ccaccccggc cggcccctgg ccggccgtcg
     2221 ccggtgtgga agtttggggt atgcctggcc ggggtcggca acgccgatca gaacgtatgg
     2281 cagatgccca cgtttccgga cgccatcaag tcggcgcgcg tcgaagcggt taagatcgtc
     2341 ccgttgaacg atctcgaaca gatgaaccgg gtgtatgagt ggttccgctc gcaaggcatc
     2401 gcttttgtca tggtgcgcct gacctggaag cccgacccgg cctgggccaa tctcccggcg
     2461 gcagcgcgca tcacggcggc ggtcaattcg tttgtgcaaa cagtgcgcgg ccagctcgag
     2521 ttcgcgtacg cgaacggcgt gcgctacttc gaagtccaca acgagccgaa catcccggcg
     2581 aacccgaccg atccggtcgg cgacgggctg ggcacggcgt ggatgggccc ggaggagttc
     2641 gcaaactggt tcggccgcgt cgtcgacgat ctgcgcaaga ttcgccccaa tgcttactac
     2701 ggcttccccg gcctcgcgcc gcgcggctca cagttcgccg acttcggcgg cgggccgata
     2761 ctcgtcaagg gccagaagct cttgtggaac acggaccagt ggctgaccgt ctgcaagccg
     2821 gtcgtcctgg cgaaggcgga ttgggtcggc gcccactgct actggcagtt tgacggcggc
     2881 ggcctgttcg ggatcgagaa tcctgattcg gggggcatgt actggcggcg ctacaaggcg
     2941 agattccccg gcaaactgct gttcatcacc gagttctcca acaacggcgg ggcgatcccg
     3001 tacgccgaga agggacggca gtacgcgaaa tacatctcgc tgctgcgccg cgagaagtcg
     3061 atcggcgcag catttggata cgccatcttc tgggcgagtg atccgaaccg cgagggctgg
     3121 gtgttcatca acgaggcggg taagtggcgg gtgaccgaaa taccgggcgc gatgggcgcg
     3181 gcgttggcgg ctaacccgac gacggcgaat ccggtggtcg tcagctcgcc ggcggtgtgg
     3241 gcgtgaatga acgtgaagcg tgatacgtga tgcgtgagac ttggtctcac gttcgtatct
     3301 tgaccgccgg gccttcggat tccgaatggc ccggcggtct tcgcttcccc agtatctggc
     3361 gtgccctaaa tcagttttga ttccggtttg ttcttgatcg cagttcagtt tagcctgacg
     3421 atatcagatt cggcccgccg cgccgtccct tcgacaggtt ccccactcca cctgccccct
     3481 gcagggggtt cggccggaga cttcgcaggg caggccctgg cacggtcaag gccgccgggg
     3541 cctgccctga gcgcagccga agggccgaag ggccgccggg cgtgggagcc cggcccgcta
     3601 actaagtcga atcagatgca caggttctaa tcatttctaa cacaagatta accgacaacc
     3661 tatcgaaata tgcgtattgt tgtggtataa gagtaccagt atcagggcaa ggatcagagc
     3721 ttcgagcggc tttgctacat attcacccag ccgtacagcg cctgggcgtt cgcctgcggg
     3781 ggatttgtca tgcttggcag ctatagtggc ggtctgcggc acgatgcact taggagaggg
     3841 agaggagaca tgcttgccat gatgcaagtg ggtgatgatc gtccacggca agacaacggc
     3901 acatgttcgt tacgtttagc cacgaccgaa atcaacctgc ccatcctgat ctgcttgctg
     3961 ggtaacttct atgtgcggaa gaggggcgaa cctgtgaggg ttagcaacgg cgggaaggcc
     4021 gaagctttgc tcagtgctct cgcacttcag ggcgattatt gtgtctcgcg cgacggactg
     4081 cttggcattc tttggcccaa cacccattct gagctggcca tccagtcgtt gaacagcctg
     4141 gtctacaccc tgcacaagcg actcggtgat gcgatcggcg gggccgcgcc cgtgttgcac
     4201 gcggacggct actaccttct caacgtcgag gcgggcgtcg gcgtggacgt ggcgtgtttt
     4261 gacgccctgg cgcaagccgg cgatcgtgaa gccgacgcag gcaaccggag tgcggccgca
     4321 gcagcctacg accgcgcttt acgcttgtat cgaggcgatg tgtgcgccgg caacgacttg
     4381 catgccgtcc ttgagcggga gcggctgcgc gctcgctatc tggccttgct gacgcacctg
     4441 gcggattatt acttcagtga aggcaactac gctgccagcc tggactacgc ccaacgcctg
     4501 ctggacaagg atccttgccg cgaggatgcc caccgcttgg ttatgcgcag ctacgtacgg
     4561 cggggtgaac gtgcccaggc cctgcgccag tatcggttgt gccaggagat cctgcgcgcc
     4621 gagttcgacg ccgtgccgga gcctgcgacg acggcattgt tcgacgcggt gcgcctgcat
     4681 ccagacagcg tttgacgctc acttacgaat ggctcataga aacttcatgc ataattcagc
     4741 ctcaactcag cctgaattga gccggacttc aggatggtgt gctatcttga attgcgtcga
     4801 cgctctaatc tcgggtgaag gtcaggctca tcataacgcc gcaagagaat catttatcag
     4861 acaaacgcta cgttacggtg atcgtgcgca tggtgatcga ccgtcgagaa cggctggttc
     4921 acggcgaggt cgtggatgtg gacggcaaag cacaaggccg gtttgacgga tggcgagaga
     4981 tgatccacac ggtgcgcgac tatctgacga accaggagtg ctgagggcga catctagggg
     5041 cagtactgtt gagataagct caaagcccgc gtccgcgcgg ttggagagcc cttgacttaa
     5101 caggattgcc tctaggcgtg acttgcagga ataacaaaac cagccgagtt ccgctcgcaa
     5161 cgttcctcgg ctggcctggg atgaaggtaa gcgcatagat gcgctcgtct tcgtgtttat
     5221 cataccagat gaacatgaag gcaacatgaa gcgaagatga agttttccga attcgagatt
     5281 caaaagtgac cgcgaaagga gatcttcagc catgatacgt ggaattgtcg gatgggccgt
     5341 gcagtttcga ttcctggtgc ttgttatcgc cgtggcgctg atagccattg gcgtcgggca
     5401 acttcgcaac atgccggtgg acgtgttgcc cgagttctcg ccgccgtacg tggagattca
     5461 gaccgaagcc ctggggctgt ccgcggaaga agtggaacag atgatcactg ttccgatgga
     5521 gcaggacttg atggccgggg tggcctggct cgacgtcatt cactccgagt cggttccggg
     5581 gctgtcgtcg gtcgtcatct acttcgagcc gggcaccgac ctgttccgtg cgcggcagat
     5641 ggtgtccgag cgcctggccc aggcggcggt cgggattccc cacgtgtcca aaccgccgac
     5701 gatgatccag cccctgtcgt cggcaagccg gttcatgatc gttgggcttt cctcgaagga
     5761 tctgtcgctg atcgagatgt cggtgctcgc ccgttggacg attgctcctc gcctgctggg
     5821 cgtacccggc gtgtccaacg tggccatctg gggcatgcgg gatcagcagt tgcaggtgct
     5881 ggtcgatccg gaacagttgc aagcgcaggg ggtttcgctc cagcaagtcg tcgagaccac
     5941 gggcaacgcg ctgtgggtgt cgccgttgac ctatttggag gcgtcgtcgc ccggcaccgg
     6001 cgggttcatc gacaccccca atcaacggct cggggtctgg cacgtcttgc ccatctcctc
     6061 gccggaggac ctggcccagg tgcccgtcga ggacgcggcg gggctgagcc tgggtgacgt
     6121 ggccgaggtg gtggaagatc atcaaccgct gatcggggac gcgatcatta acgacggtcc
     6181 cagcctcctg ctcgtcgtcg aaaaactacc cggcgtcaac accctggagg taacgcgggg
     6241 ggtggaagaa gcgctcgccg tgttgcagcc cggtatgaca gggatcgagt ttgacgccac
     6301 cctgttccgg ccggcgacct acatcgagat ggcgatcgcc aatctcagca atgcgctggt
     6361 cgtcggcgcc gttctcgtgg tggtggctct gtttgccttc ctgtggaact ggcgtgtcgc
     6421 gttgatcagc gccgtggtca tcccgttgtc gctgatggcg tccctgctcg tgctctacgg
     6481 ccgtggggca accctcaacg caatagtcct ggcggggctg gtggtagccc tcggcaccat
     6541 catcgacgac gcgatcgtcg acgtagacaa catcgcgcgg cgtctccgcc agttgcgcaa
     6601 agagggcagt gataagtcgc ctgtgctgat tatccttagg acctcggccg agatgcgcag
     6661 gagcatcctc ttcgcgctgg tgatcacggt gctggctgtg ctgccggtct tcttcatgga
     6721 gggtgcctcc ggcgcattgt ttcagtcgct ggtcacgtcc tatgtgctgg cggtgctggt
     6781 ctccatggtg gtggcgctgc tcgtcacgcc cgcgttgagc atgatcttct tggccaatgc
     6841 gccgctcgag cgccgtcagt cgcctctggt gggttggctg cagcgtggct acgaccggtt
     6901 tctggcgcgg atcctccaca acgcgcgcct ggcgtacgtc gccgtcgcgg tcatcgtgct
     6961 ggccggcgtg gcggcgctgc catttctgga gcagcagtcc ctgctgccca cgttcaaaga
     7021 accgtacctc tcgatccagt tggagggtgc acccggcacg tcccgtccgg aaatggaccg
     7081 gatcgtggcc cgggtcagtc aggagttgcg gaccattcct ggcgtcagca acgtcggcgc
     7141 ccacgtcgga cgcgccgtgt ttggggatca agtcgtcggt atcaattccg cccagctatg
     7201 ggtcagcatc gatcctgcgg ccgactatga tgcgacggtg gccgccgttc aggagacggt
     7261 ggacggctac gccgggctgg gtggcgaggt gagcacctat ttgcagcaga cgctcagcca
     7321 gccacggacg agcgccggcg acggcattac cgtgcgcgtc tttggcgagg acctggacgt
     7381 cctgcgcagc gaagcggaca aggtgcggca ggccctggta gggattgccg gcgtcgtcga
     7441 tccgcacgtg gtgctcccgg ttgaggaggc caccctggaa atcgaggttg acctcgactc
     7501 ggcccagact catggcatca agccgggcga tgtgcgccgg gcggcggcca tcttgttgtc
     7561 gggccttcag gtcggcagcc tgttcgaaga acagaaggtg ttcgacgtag tcgtgtgggg
     7621 cacacccgaa acacgccaga gtctgaccac tgttcgcgat ctgctgatcg acacgcccgg
     7681 cggcgatcag gtgcgtttgg gagatgtggc cgacgtgcgc ctcacgtcga gtccgatcgt
     7741 catacgacgt gaagccgtct cgccttacct tgacgttgtc ttcaacgtgg ggggacgcga
     7801 ccccctggcc gtgacgcgcg atgtcgagac ggccctgcag aacgtcccgt tctcgctgga
     7861 gtaccacgcg gagctgcagc acgactacga agcgtcacag gctgcgaatc agcgcacgct
     7921 cctcaccgga gccgtcgcgg tgatcgggat tttcctcctc ttgcaggcgg cctacgggag
     7981 ttggcgcctg gcggctgtcg ccatcttgac cttgcccatg gcactggcgg gcggcgtgct
     8041 ggcggcgctc ctcgccggcg gtctctcgct cggttcgctg tttggtttgc ttatggtgtt
     8101 ggggatcgcg gtgcgtaacg gcattgtgat gaccaaccac tttcagtatc tggagcagcg
     8161 tgaagaccag gccttcgggc cggagcttgt cctgcgcggg gcgcgcgagc ggctggcgcc
     8221 gattgtgatg acggcgttag ccaccggact ggccctcgcg cccttcgtgc tcttcggcgc
     8281 cgttcccggt ctcgagatcg tgcgcccgat ggccatcgtc attctggggg ggctggtcac
     8341 ttcgaccttg ctcgacctgt tcgtg
//
LOCUS       DBNHXB010000249         3400 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1737637, whole genome shotgun sequence.
ACCESSION   DBNHXB010000249 DBNHXB010000000
VERSION     DBNHXB010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1737637"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="AC162G_10225"
     CDS             complement(<1..272)
                     /locus_tag="AC162G_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR5076236.1"
                     /translation="MKWEYVELHTHSYYSLLDGVPSPEELVEQAARYEMPALALTDHD
                     ALYGLPRFVLAAEQAGIKPIIGVELTLANGGGHLTLLAENSAGYANL"
     gene            310..567
                     /locus_tag="AC162G_10230"
     CDS             310..567
                     /locus_tag="AC162G_10230"
                     /inference="COORDINATES: protein motif:HMM:NF014059.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HIR5076237.1"
                     /translation="MTVKSVTAIYQKGMLKPLSSLPLVENERVEVSITRSETNNRPVL
                     TLSGVWKGLGDPSYEDIVAVTHAAHRARLERLSSLLDTTGS"
     gene            574..978
                     /locus_tag="AC162G_10235"
     CDS             574..978
                     /locus_tag="AC162G_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR5076238.1"
                     /translation="MADLYVADTHALVWHLFNPTRLSAPAAEAFARVDRGEAILHVPL
                     VVVAETAMVIEKGRVQATLDQLDALLHQMAGSRNYQIGALDLDTVLAATRLTELTDIF
                     DRLLVAEVRALGAALITRDEEITAARAVPVIW"
     gene            complement(989..2383)
                     /locus_tag="AC162G_10240"
     CDS             complement(989..2383)
                     /locus_tag="AC162G_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR5076239.1"
                     /translation="MRFACLLVEHLPTRVEALCSPDLAAKSLVVLRAWDDSVIDASPE
                     LGAKGIGPGDSRRRVDQLCPDAIILSAREEVYQAHHDKLRNGLLNFASAVETSALGEL
                     FIEVGALSRAFPSDKALALQIATQTQRDTRLLPTVGIASNKFTAARAARLAAQESSRA
                     LIVPDGQERAFLAPLSLRSLPDPPLEMLRRLHLFGINTLGDFAQLPHAAAALQFGADL
                     AFYHDLACGRDPRPLAPQSPPPMVTHTLRLPEPIADRHMALAALEHLAGQMAHDLDKA
                     GYHAVAMSLAVLTSDGQEHTVGGSIKPPSSDASLLRRLAGRLLGKLSFSVEVTCLTLT
                     AYPLREWHLGARQLTLFEEALQPKLARIQEVLRLLRQRFGEAVIRLASMIGPPVPIPI
                     RVRAEADGTPSVLSWSGWSRPVERVYEYWREMRTWWSAPIERDYYQVVVVGEAVYTVF
                     CDGKGRWFLDRRRG"
     gene            2647..>3400
                     /locus_tag="AC162G_10245"
     CDS             2647..>3400
                     /locus_tag="AC162G_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR5076240.1"
                     /translation="MNFVQALGLPAFALTDTNALYGAVEFASRCRDAGIRPIIGAELT
                     VAGGHSVVFLAQDREGYANLCHLVTRLQAAPDREASLARGLALSDLDGHTHGLIALSG
                     GCAGLLDAALREGDGQRAESLVQDFTNLFGRDRFFVELQIIEAGDGAKAAVLHSLAGR
                     MGVRTVATHDIRYLSPGDAPRYRVLAAMRQSQSLKDLPSMPDLSFPVPEDMQRRFRDF
                     PTALDNTNLVAEMCRFDLPLGELHFPTLDLPPG"
BASE COUNT          663 a         1091 c         1007 g          639 t
ORIGIN      
        1 agattggcgt agccggcgct gttttcagct aacagtgtga gatgaccgcc cccgttggct
       61 aacgtcagtt caacaccgat gatcggcttg atgccggctt gctctgcggc taacacaaaa
      121 cgcggtaagc cgtagagcgc gtcgtgatcc gtcaaggcca aggccggcat ctcgtatcgc
      181 gcggcctgct cgaccaactc ctctggcgaa ggcacgccgt ccagcaacga gtagtacgag
      241 tgggtatgca gctcaacgta ttcccatttc ataagttagc cggtataatt agcgcggagg
      301 gagttcagca tgacggtcaa gtctgtaacg gcaatctacc aaaagggcat gctcaaacct
      361 ctatcctcgc ttcctctggt cgagaacgag cgggtcgagg tcagcattac gcggtcagag
      421 acgaacaacc gtcctgtgtt aacgctatcc ggcgtctgga agggactggg cgacccgtcc
      481 tatgaagaca tcgttgcggt gacacacgcc gcacatcggg cacgactgga acgcctatcg
      541 tcacttttgg acacgacagg ttcgtaacgt cgaatggccg atctctacgt ggccgacacc
      601 cacgcgctgg tctggcactt attcaatccc acccgcttaa gcgcgccggc cgccgaagcc
      661 tttgcccgag tcgaccgtgg cgaggcgatt ctccatgtgc ccctggtggt cgtcgctgaa
      721 acggcgatgg tgatcgagaa aggtcgcgtg caggccacgc tcgatcagct cgatgcgctc
      781 ctccaccaaa tggccggcag ccgtaactac cagattggcg cactcgacct cgacacagtg
      841 ctggccgcca cccgcctgac tgaactgacg gacatctttg accgcctgct cgtcgccgag
      901 gtgcgtgcgc tgggtgccgc gctaatcacg cgcgatgaag aaatcactgc cgcccgcgcc
      961 gtgcctgtta tctggtagat ctattccttt atcccctgcg ccgatccaga aaccagcggc
     1021 ccttgccatc gcaaaacacg gtgtacacgg cctctccgac gaccaccacc tggtaatagt
     1081 cgcgctcgat gggcgcgctc caccacgtcc tcatctcgcg ccagtattcg tacacgcgct
     1141 cgaccggacg cgaccagccg ctccagctca aaaccgacgg cgtgccgtct gcttcggcac
     1201 gcacacggat cggaatgggc acgggcgggc caatcatgct ggccaatcga atgaccgctt
     1261 cgccgaaacg ttggcgcagc agccgcaaga cctcctgtat ccgcgccagc tttggttgga
     1321 gggcctcctc gaacagcgtg agctgcctgg cgcccagatg ccactcgcgc aagggatagg
     1381 ccgtgagcgt caggcaggtc acttcaaccg agaacgatag cttgcccagc agccggcccg
     1441 ccagccggcg tagaagactc gcatccgagg agggaggctt gatcgaccca ccgacggtgt
     1501 gctcttgtcc atcgctcgtc aacacggcca gtgacatcgc cacggcgtga tagccggcct
     1561 tgtcgagatc gtgcgccatc tgccccgcca ggtgttccag ggcggccaac gccatatgcc
     1621 gatccgcaat cggctcgggc agtctgagtg tgtgggtgac catcggcggc ggtgattgtg
     1681 gagcgagtgg acgcggatcg cgcccgcagg cgagatcgtg gtaaaaggcc aggtctgcgc
     1741 cgaattgtag agcggcggcg gcgtggggca gttgtgcaaa gtcgccgagt gtgttgatcc
     1801 cgaacaggtg cagccggcga agcatttcca acggcggatc gggcaatgac cgaagcgaca
     1861 gcggcgcgag gaaagcgcgc tcttggccat caggcacaat cagcgcgcgg ctgctctctt
     1921 gcgcggcgag tcgtgcggcc cgagccgccg tgaatttgtt ggacgctatg ccgactgtag
     1981 gcaaaaggcg cgtatctcgc tgggtctgtg tggcgatctg cagagccagc gccttatccg
     2041 atgggaatgc ccgcgacagc gcgccgactt cgatgaagag ttcacccagc gctgaggttt
     2101 cgacggcgct cgcaaagttc aaaagcccat tcctgagttt gtcgtgatgg gcctgataga
     2161 cctcttcgcg ggcggacaga atgatcgcat ctgggcaaag ctgatcgaca cggcggcgcg
     2221 aatcgcctgg gccgatgcct tttgccccga gttcaggcga tgcgtcgatg acgctatcat
     2281 cccaggcacg aagcacgacc agtgactttg ccgcgaggtc agggctgcat agtgcttcaa
     2341 cacgcgtggg caggtgttcg accagcagac aggcaaagcg cattcacgcc tttcactctc
     2401 acaatcgaga gaaacaggcc aagtttgatg cgcccaactt agtcgcaaca gaattgaaac
     2461 atttgttcta atactaacat gaaagggtat gatgtcaacc cccatccgca accgaaaact
     2521 cacctgtaga tcatcacttg cccggtgttc gacgcgatca tataatcttt gcatatgcac
     2581 gccatccccc tgcatctcca ttcccattgg agcctcctcg atggggtccc ttccgttcac
     2641 gagagcgtga acttcgtcca ggcgctcggc cttccggcat tcgccctgac cgacacgaat
     2701 gcgctgtacg gggcggtgga gttcgcgtca cgttgccgag acgccggtat tcgtccgatc
     2761 atcggcgcgg aactgaccgt tgcaggtgga cattccgtcg tcttcctggc acaggaccgg
     2821 gagggctacg ccaacctctg ccacctggtc acgcgactgc aagccgcgcc ggatcgcgaa
     2881 gcgtcgctgg cgcgcggact cgcgctttcc gacctcgacg ggcataccca cgggctgatc
     2941 gctttgtccg gcggctgcgc gggtctactc gacgccgccc tgcgcgaagg cgacggacag
     3001 cgcgccgaaa gtcttgtgca agacttcaca aatctattcg ggcgcgaccg cttcttcgtc
     3061 gagctgcaga tcatcgaagc aggcgatggg gcgaaagccg ccgtgcttca ctcactggcc
     3121 gggcgcatgg gcgtccggac tgtcgcgact cacgacattc gatacctgtc gcccggcgac
     3181 gcgccgcgct accgcgtgct cgcggccatg cgccagagcc aaagcctcaa agatctgccg
     3241 tccatgcccg acctgtcttt ccccgtgccc gaggacatgc agcgcaggtt ccgcgacttc
     3301 cccaccgcgc tcgacaacac aaacctggtc gccgaaatgt gccgcttcga tctgccgctt
     3361 ggcgagcttc acttccccac gctcgatctg ccgccgggcc
//
LOCUS       DBNHXB010000250         6558 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2409933, whole genome shotgun sequence.
ACCESSION   DBNHXB010000250 DBNHXB010000000
VERSION     DBNHXB010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2409933"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..496)
                     /locus_tag="AC162G_10250"
     CDS             complement(74..496)
                     /locus_tag="AC162G_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR5076241.1"
                     /translation="MTRPIRVLIAKPGLDGHDRGAKVVALALRDAGFEVIYTGLHQAV
                     EQIVEAALQEDVDAIGLSILSGAHLPIARRLMDRLRAANAEDVVVLVGGNIPERDVEP
                     LKALGVAGVFPTSSRFADIAAFIRERVTASEAVSHAGR"
     gene            complement(612..1823)
                     /locus_tag="AC162G_10255"
     CDS             complement(612..1823)
                     /locus_tag="AC162G_10255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR5076242.1"
                     /translation="MQFQNAFVPYGGYWCTPFVKWQGNFANLHPLTFAADVARRALAE
                     RQIDPRAFDALFLGMTVPARNSFYGAPWVAGMIGAEGVTGPTFGQACATSARVIGSAA
                     FEIEAGNGTRNTILCLTADRTSNGPHMVYPNPAGPGGKPDVEDWVWDNFNCDPFAGNA
                     MIETAENVAREEEITTEEQHEVALLRYRQYQDALNDDAAFHRRYMVMPVEVNPSGRKV
                     IATVKDDEGVFATTAEGLAKLKPVLKDGTVTFGAQTYPADGNAGLIVTTKERAEELSQ
                     NPKVKIQILSFAQGRAKKGFMPMANLPATQRALALAGIVIADVKAIKTHNPFAVNDVY
                     FARAMGVKKEAMNNYGSSLVWGHPQAPTGMRLVIELIEELVLLGGGYGLFTGCAAGDT
                     AAAVVVKVSDR"
     gene            complement(1884..3107)
                     /locus_tag="AC162G_10260"
     CDS             complement(1884..3107)
                     /locus_tag="AC162G_10260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR5076243.1"
                     /translation="MREAVIVEAVRTPIGRHGGVLKDVRPDDLAALVITKVLERVSVD
                     PALVEEVYFGCANQAGEDNRNVARMGMLLAGLPVSVAAVTFNRLCASGLAAINAAARA
                     IKVGEGDVYIAGGVESMTRAPYAIPKTSPEWAFGNVTAWDTTIGWRFPNQKLKERYGN
                     DSMGETAENVYELTKTISREEQDQFALASHQRAVAAIDSGKFAEEIVPVSIPQRKGDP
                     VLVSRDEHPRPDTSMQALGKLRPAFRQGGTVTAGNSSGINDGAAAVLLMSAEKARELG
                     KKPLARIVASAAAGVEPRTMGLGPIPATRKALERAGLKIEDIGLIELNEAFAVQALAV
                     MKELGFRHEITNVNGGAVALGHPLGCSGARIVCTLVHEMQRRAANEKRPYYGLATLCV
                     GVGQGETTIVEWVGG"
     gene            complement(3195..3938)
                     /gene="fabG"
                     /locus_tag="AC162G_10265"
     CDS             complement(3195..3938)
                     /gene="fabG"
                     /locus_tag="AC162G_10265"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR5076244.1"
                     /translation="MDVQGKGAIGTGGSLGIGRAIALDLAAHGANIAINYRKHADEAN
                     QVVCEAERMGRRGLAVQADVASFGDALSMVDAVRQKLGRLDILVNNAGINRDAVVWKM
                     DETQWDEVMATNLKGYFNYIHAAAPIFKEQGSGKIVNITSINGLRGKFGQSNYSAAKA
                     GIIGLTKAVARELGKFNVNVNAVAPGLIETEMMRQAPQDVKDKALAEIVLGRLGVPEE
                     VAHVVTFLCSDLARHITGEVIKVDGGQYI"
     gene            complement(3940..4707)
                     /locus_tag="AC162G_10270"
     CDS             complement(3940..4707)
                     /locus_tag="AC162G_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HIR5076245.1"
                     /translation="MRLKDKVALITGGGSGIGRATALRFAREGACVVVSDLDEAGVNE
                     TAHLVVEAGGKAVAVVGNVSARADAQKMVEAATSHFGRLDILINNAGINRDALAVRVK
                     DGDVKMMSEEQWDSVMAVNLKGTFLCAQAASVPMMAQKHGRIVNTSSIGALGNVGQAN
                     YSASKAGVIGLTKTLALEWARFNITVNCVAPGATKTRMTAGIPENIMAGLLEKIPFRR
                     LAEPEEIAAAHVFFASDDAGYVTGQVLFVDGGISVGV"
     gene            complement(4813..5268)
                     /locus_tag="AC162G_10275"
     CDS             complement(4813..5268)
                     /locus_tag="AC162G_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR5076246.1"
                     /translation="MTYKTRGLTFDQFNLGDTFVSQARTVTEADVVNFAGLSGDFNPL
                     HTDEQFGKTTPFGGRIAHGMLVAAMATGMANWTGVFEGTTIALMEQVIQYKGAVKFGD
                     TVHLELKVAEKKETSKPDRGVVVFETRVCNQEGKAVIEGKWTLMMKRSQ"
     gene            complement(5289..6428)
                     /locus_tag="AC162G_10280"
     CDS             complement(5289..6428)
                     /locus_tag="AC162G_10280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5076247.1"
                     /translation="MDFALTEEQELFRKSVRDFVEKEVIPVAREIDERGEFPMALFRR
                     CGEFGYFGLRYPESAGGMGAGFTTFCLMAEEIARGSLALAAAVSMQSLMGTDFVYRFG
                     TDDHKARLLAPAFGGEKIGTIAMTEPDFGSDLGGITTRAIRDGNGWVLSGRKMWITSA
                     TVADFFTVAAKTDPDAGFKGIDMFLVERGMPGLSVGKRIEKMGVRASETSELILESVR
                     VPPENLLGEQGTGFKNLSAILGEIRTMTGALALGLAQAALDASIRYSHERVQFGKPIG
                     AFQAIAHKIAEAGTQLEAARGLVYRAAWLVDSGKPDMKLASMAKLFATEMANKVADEC
                     TRIFGSYGFAMEYDAQRYFRDARFLLYGGGTSEILRGIIAKEMGV"
BASE COUNT         1130 a         2297 c         1814 g         1317 t
ORIGIN      
        1 attcctcacg agtcttatca aagcatcggt ccgggccgag ccggtgaccg tctgaaagaa
       61 gccgacgaca tcatcatcga cccgcgtgtg acacagcttc gcttgctgtg acacgctcgc
      121 gaatgaatgc cgcgatgtcc gcgaatcggc tgctcgtcgg gaacacgcct gccacgccga
      181 gcgccttgag cggctcgacg tcgcgctctg ggatgttgcc gccgacgagc acgaccacgt
      241 cctcggcgtt cgccgcgcgt aatcgatcca tcagcctgcg cgcgatcggc aggtgcgcgc
      301 ctgacaggat cgacagcccg atcgcatcca catcttcctg cagtgccgcc tcgacaatct
      361 gctcgacggc ctggtgcagg ccggtgtaga tcacctcgaa gccggcgtcg cgcagcgcca
      421 gcgccacgac cttcgccccg cgatcatgac catccaatcc gggtttggcg atgagaacgc
      481 gaatgggtct tgtcatgttg tcacagttgc ccgtagttag tgatcagtta tcagttatca
      541 gtgatcagtt gtcagtggcc gattgatacg actgatgact gacccaccaa ttactgatta
      601 ctgatcactg atcaccgatc acttaccttc accaccaccg ccgccgccgt gtcgcccgcc
      661 gcgcagccgg tgaacaggcc gtacccgccg ccgagcagca ccagctcctc gatcagctcg
      721 atgacgaggc gcatcccggt cggtgcctgc gggtgccccc acaccagcga cgagccgtag
      781 ttgttcatcg cctccttctt cacccccatc gcgcgcgcga aataaacgtc gttgacggcg
      841 aagggattgt gcgtcttgat cgccttcacg tcggcgatga cgatgccggc cagcgccagt
      901 gcgcgctgcg tggccggcag gttcgccatc ggcatgaagc ccttcttggc gcggccctgc
      961 gcgaaactga gaatttggat tttgaccttt ggattttgac tcagttcttc cgcccgttct
     1021 ttcgtcgtca cgatcaagcc ggcgttgccg tcggccggat aggtctgcgc gccgaacgtc
     1081 accgtgccgt ccttcaacac gggcttaagc ttggccagtc cctcggcggt agtcgcaaac
     1141 accccctcgt cgtccttgac cgtggcgatg acctttcggc cagaaggatt cacttccact
     1201 ggcatcacca tgtagcgcct gtggaaggca gcgtcgtcgt tcagcgcgtc ttgatattgc
     1261 cggtagcgca ggagcgcgac ctcgtgctgc tcttcggtcg tgatctcctc ctcgcgggcg
     1321 acgttctcgg ccgtctcgat catcgcgttg cctgcgaacg gatcacagtt gaagttgtcc
     1381 catacccagt cctcgacgtc gggcttgccg cccgggccgg ccgggttggg gtacaccatg
     1441 tgcgggccgt tcgacgtgcg atcggcggta aggcatagga tcgtgttacg tgttccgttt
     1501 cccgcttcga tctcaaaggc tgcgctgccg atcacccgcg ccgatgtcgc gcacgcctgg
     1561 ccgaacgtcg ggccggtgac gccttcggcg ccgatcatac ccgccaccca cggcgcgccg
     1621 taaaacgaat tcctcgccgg gacggtcatg cccaagaaca acgcatcgaa agccctggga
     1681 tcgatctgcc gctccgccag cgcgcgcctg gcgacgtcgg cggcaaacgt caagggatgc
     1741 aggttggcga aattcccctg ccacttcacg aacggcgtgc accagtagcc gccataagga
     1801 acgaaggcat tctggaattg catctctctc tccttcacgt ttcacgcttc acgttttacg
     1861 ttttgcgctt cacgtttcac gcatcaaccc cccacccact ccacgattgt cgtttccccc
     1921 tgccccacgc ccacgcacag cgtcgccagc ccatagtaag gtcgcttctc gttcgcggcg
     1981 cggcgctgca tctcgtgcac cagcgtgcac acgatgcgcg cgcccgagca gccgagcgga
     2041 tgacccagcg cgaccgcccc gccgttgacg ttggtgatct cgtgccgaaa gccaagctcc
     2101 ttcatcacgg ccagcgcctg cactgcaaac gcttcgttca gctcgatcag cccgatgtct
     2161 tcgatcttca acccggcgcg ttccagcgcc ttgcgcgtcg ccgggatagg cccgaggccc
     2221 atcgtgcgcg gctcgacgcc cgccgccgca ctcgccacga tgcgagccaa cggcttcttg
     2281 cccagctcgc gcgccttctc cgcactcatc agcagcacgg ccgccgcgcc gtcgttgatc
     2341 ccgctcgagt tgcccgccgt caccgtcccg ccctggcgaa acgccggtcg cagcttgccc
     2401 agggcttgca tcgaggtgtc ggggcgcgga tgctcgtcgc ggctgaccag gaccggatcg
     2461 cctttcctct gcggaatcga aacgggcacg atctcttcgg cgaacttgcc agaatcgatc
     2521 gccgccaccg cgcgttgatg ggacgccaag gcgaattggt cttgctcttc gcgcgagata
     2581 gtcttggtca actcgtacac gttctcggcc gtctccccca tcgagtcgtt gccataccgc
     2641 tccttgagtt tctggttcgg gaagcgccag ccgatcgtcg tgtcccaggc tgtcacattg
     2701 ccgaaggccc actcggggct ggtcttgggg atcgcgtacg gcgcgcgggt catgctctcg
     2761 acgccgcccg caatgtacac atcgccctcg ccgaccttga tcgcgcgcgc cgccgcatta
     2821 atcgcagcca ggcccgaagc gcataaacga ttgaacgtca ccgccgccac tgagaccgga
     2881 agcccggcca gcagcatacc catgcgcgcc acgttccgat tgtcttcgcc ggcctggttc
     2941 gcgcaaccga aatacacttc ctcaaccagt gctggatcga cgctcacgcg ctcgaggact
     3001 ttcgtaatca ccaacgcagc cagatcgtcc ggccgcacgt ccttcagcac gccgccgtgc
     3061 cgcccgatcg gcgtccgcac tgcttctact attactgcct ctctcatatt gcctccaatt
     3121 tcaccacgtc ctgacggttc cagatgacgt cttcggctgc ctcgcttcac gcatcacgca
     3181 ccacgcttca cgtcttaaat atactgccca ccatccacct tgatgacttc acccgtgatg
     3241 tgccgcgcga gatcggagca gaggaacgtg acgacatgcg cgacttcttc gggcacgccc
     3301 agccggccca gcacgatctc ggccagcgcc ttgtccttga cgtcttgcgg cgcttgtcgc
     3361 atcatctccg tctcgatcag gcccggcgcg acggcgttga cgttgacgtt gaacttgcct
     3421 agctcgcgcg cgacggcctt agtcaatccg atgatcccgg ccttggccgc cgagtagttg
     3481 gactggccga acttgccgcg cagtccgttg atcgaggtga tgttgacgat cttgcccgag
     3541 ccttgctcct tgaagatcgg cgcggcggcg tgaatgtagt tgaagtagcc cttgagattg
     3601 gtggccatga cttcatccca ctgggtctcg tccatcttcc acaccacggc gtcgcgattg
     3661 atgcccgcgt tgttcaccag gatgtccagc cggccgagct tctggcggac ggcatcgacc
     3721 atgctcagcg cgtcgccgaa cgacgccacg tcggcctgca cagccagccc gcgccgcccc
     3781 atgcgctcgg cctcgcagac aacctggttg gcttcatcgg cgtgcttccg ataattgatc
     3841 gcaatattcg cgccatgggc ggccaggtcg agcgcgatcg cgcgcccgat gcctaagctg
     3901 ccgccggtcc caatcgcccc tttgccttgc acatccatct cacaccccca cgctgattcc
     3961 gccgtcgacg aacagcacct gcccggtgac gtagcccgca tcgtcgctgg cgaagaacac
     4021 atgtgccgca gcaatttcct caggctcggc cagacgacgg aacgggattt tctccagcag
     4081 gccggccatg atgttctccg ggatgcccgc cgtcatgcgc gtcttcgtcg cccccggcgc
     4141 gacgcagttg acggtgatgt tgaatcgcgc ccattcgagc gccagcgtct tcgtcaggcc
     4201 gatcacgccc gctttcgacg ccgagtagtt cgcctgcccc acgttgccga gcgcgccgat
     4261 cgaagacgtg ttcacgatgc gcccgtgctt ctgcgccatc atcggcacgc tggccgcctg
     4321 cgcacacaga aacgtgcctt tgagattcac ggccataacc gagtcccact gctcttcgga
     4381 catcattttc acgtcgccgt ccttcacgcg cacggccagc gcgtcccggt tgatgcccgc
     4441 gttgttaatg aggatatcca gccgcccgaa gtggctcgtg gccgcctcga ccatcttctg
     4501 agcatcggcg cgcgcggaca cattgccgac gacggcgacg gccttgccgc ccgcttcgac
     4561 gacgagatgg gccgtctcat tcaccccggc ctcgtcgagg tccgacacca cgacgcacgc
     4621 gccttcccga gcgaagcgga gcgccgtcgc ccgcccgatg ccgctcccgc cgccggtaat
     4681 gagtgctact ttgtccttga gtcgcatgac gttctgtcct ctccgtaatc agtaatcagt
     4741 gatcggtgat cggtgatcag tggctacgga ttactgatga ctgatgacgg gtcactgatg
     4801 actggctact ctttactgag atcgcttcat catcagcgtc cacttgcctt cgatgacggc
     4861 cttgccctct tgattgcaca cgcgcgtctc gaagacgacc acaccgcgat ccggcttgga
     4921 ggtttccttc ttctcggcca ctttcagttc gagatggacc gtgtcgccga acttcaccgc
     4981 gcccttgtac tggatcacct gctccatgag cgcgatggtc gtaccttcga acacgcccgt
     5041 ccagttggcc atgcccgtcg ccatggctgc gacgagcatg ccatgagcga tgcgtccgcc
     5101 gaacggcgtg gtcttgccaa actgttcatc ggtgtgcagc ggattgaagt cgcctgatag
     5161 cccagcgaag ttcaccacgt cggcctcggt gacggtgcgc gcctgcgaga cgaacgtgtc
     5221 gcccagattg aactgatcga acgtcaggcc gcgagtcttg tatgtcattc agaatctccc
     5281 tttctccatc aaactcccat ctccttcgcg ataatgcccc tcaagatttc gcttgttccc
     5341 ccgccataga gcaagaagcg cgcgtcgcgg aagtaacgct gcgcatcgta ctccatggcg
     5401 aagccgtaac tgccgaaaat acgcgtgcat tcgtcggcaa ccttgttcgc catctcggtc
     5461 gcgaacagct tcgccatcga cgccagtttc atatccggct tgccgctgtc gacgagccat
     5521 gcagcacgat acaccaatcc gcgcgccgcc tccaattgtg tgcccgcctc ggcgatcttg
     5581 tgtgcaatcg cctgaaaggc gccgatcggc ttgccgaact gcacgcgctc gtgcgagtat
     5641 cgaatcgacg cgtcgagcgc ggcctgcgcc agccccagcg ccagcgcgcc ggtcatcgtg
     5701 cgtatctccc ccaggatcgc gctgaggttc ttgaagcccg tgccctgctc gcccagcagg
     5761 ttctcgggcg gcacacgcac gctctccaaa atcaactccg acgtctccga cgcgcgcacg
     5821 cccatcttct cgatccgctt gccgaccgac agccccggca tgcctcgttc gaccaggaac
     5881 atgtcgatgc ccttaaaccc ggcgtcggga tcggtcttgg cagccacggt gaagaagtcg
     5941 gcaacggtgg cagaggtaat ccacatcttg cgtccgctca acacccagcc attgccatct
     6001 cggatcgctc gagtggtgat gccgcccagg tccgagccga aatccggctc cgtcatggcg
     6061 atcgtgccga tcttctcgcc gccgaacgcg ggcgcgagca gacgtgcctt gtgatcgtcc
     6121 gtaccgaacc gatacacgaa atccgtcccc atcaggctct gcatcgagac cgccgccgcc
     6181 agcgcgagcg agccgcgcgc gatctcctcg gccatcaggc agaacgtcgt gaagccggcg
     6241 cccatcccgc cggccgattc cggatagcgc agcccaaagt agccgaactc gccgcagcgc
     6301 cggaacagcg ccatcgggaa ctctccccgc tcgtcgatct cgcgcgcgac cgggataact
     6361 tccttttcga cgaagtcgcg cacggacttg cgaaacagtt cttgttcttc ggtcagagcg
     6421 aagtccatat gactaattac tgattactga tcactgatca ctgactactg atcgccttct
     6481 tctcggccag atggatcaac acgccaaacg catcctttgg atggatgaac gcctcgcgcc
     6541 agacacagcc ctcgaatg
//
LOCUS       DBNHXB010000251         4087 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3751980, whole genome shotgun sequence.
ACCESSION   DBNHXB010000251 DBNHXB010000000
VERSION     DBNHXB010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3751980"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1888)
                     /locus_tag="AC162G_10285"
     CDS             complement(<1..1888)
                     /locus_tag="AC162G_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HIR5076248.1"
                     /translation="MTIALFGLLAIVLAAGGLALLYVASVSATVPTVRIGQIQAAMNY
                     AYVRIEGVVRRSPAVNPEAESLSFWVDDGSGQMLVTAFRPAGRALIEADRVPSIGDRV
                     VVEGALRVRDETPSLTLSTIDALAITRVTDSASPREISSITPDDALAPVTVRGQVRAV
                     RQPYDGLRLVTLRDASGEIDLAVERDAAKAGAPAPDIRVGDPIEATGVVTLFKGTPQV
                     TVTRGDHVAVLDQPVVFTEQEPIGSLGDDDIGRWVRVQGTIAGIAPFSAGVKFTLSDP
                     QGRDVTLLLWHDVFGALPDAADWQIGAEVVAQGPVNSFRGDLEIVPELPSDVSVLTRA
                     VAEEPATLRLAPLGAITPEDIGRTVFISGTIESIDRFENGVRFRLEDDTGSIRLVLFS
                     DVYDQLENGDILREGVSVSALGRVSEFSGALEVVPPNGASVRAWVGAQVAEAAPTPRL
                     PVVTATPRPTPEALPTMPTIILADVMTPTAKPTPGAIPTVATTPTPASGAVTAINTID
                     NTSIGQVVTVRGRVVETYSFSAGFRFIIDDGTGSIALVLFDGRYREVEERAALNLGAR
                     VAVEAEVVEYNGALELQPASGEKVVVEEPGSTASIETRAINTLSRVDIGRLATVAGDV
                     LRVEG"
     gene            2163..2537
                     /locus_tag="AC162G_10290"
     CDS             2163..2537
                     /locus_tag="AC162G_10290"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5076249.1"
                     /translation="MALHISAEQMAQYKRTARARWQAEREQQDARRERAWKLARQAAD
                     LLKREYGVRRVMVFGSLTHSDRFTLWSDVDVAAWGLTPANWLSAIGAVNALSSEIELN
                     VVDVECCPPQLLAAIEREGVSL"
     gene            2534..3040
                     /locus_tag="AC162G_10295"
     CDS             2534..3040
                     /locus_tag="AC162G_10295"
                     /inference="COORDINATES: protein motif:HMM:NF044627.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease toxin HepT-like protein"
                     /protein_id="HIR5076250.1"
                     /translation="MIPRYRLLAERVRSELRSLEQVVERANNALARTIPHPQDQDYFL
                     AAAALDLHGFYVGLERLFELIATEIDRSRPGGPTWHRDLLAQMSLTVPDVRPAVVSDE
                     TRTKLRNYLEFRHVVRNVYTFDLQPDRVTELVGGLRPAFDLARRDLLAFADFLEELST
                     ADEEDPAS"
     gene            complement(3121..3894)
                     /locus_tag="AC162G_10300"
     CDS             complement(3121..3894)
                     /locus_tag="AC162G_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076251.1"
                     /translation="MKGFHVLVAVGLLAALISVAPASAASAVSIGIYSIAACKDETTV
                     VVNGSSTYSNNRLDVGVYHKNNKGQYVLLKQTYTSAFGAGSFMLAVPLAYPESGTSEG
                     ETLRIDVQLQYLSGSSYVNVGPLLIQHVTVADKYCIGKCTVTVDTADKAPASGTLTLR
                     SHFGQWFRPEGWLHGAVPVSAGQKVRAVFVGLSCGWTVRAWYYPKSGDKTPKMLPSQY
                     WPNEFAANSLDGTNPYTTAFAKGLKATHPLEPDDPFITK"
BASE COUNT          739 a         1303 c         1321 g          724 t
ORIGIN      
        1 agccttccac gcgcagcacg tcgccggcga ctgtcgctag cctgccgatg tccactctcg
       61 acagtgtgtt aatcgctcgc gtctcgatgc tcgccgtcga gccgggctcc tcgacgacga
      121 ccttctcgcc cgaggcgggc tgcaattcga gagcgccgtt gtactcgacc acttcggcct
      181 ccacggcgac ccgcgcgccg aggttcagcg cagctcgctc ttccacctcg cgataccggc
      241 cgtcaaacaa gacgagcgcg atcgagccgg tgccgtcgtc gatgatgaaa cggaatccgg
      301 cggagaagct gtacgtttcg acgacccggc cgcgcaccgt caccacctgc ccgatcgacg
      361 tgttgtcgat cgtattgatc gccgtcactg cgccggacgc cggcgtaggc gtcgtggcga
      421 cggtcgggat ggcccccggt gtgggcttcg ccgtcggcgt catgacgtcg gctaagatga
      481 tcgtcggcat ggttggcaga gcttcgggcg tcggtcgcgg cgtggcagta acgaccggca
      541 gccgtggcgt cggcgcggcc tcggcgacct gcgccccaac ccaggcacgc acgctggcgc
      601 cgttgggcgg gacgacttcg agcgcgccac tgaactcgct cacgcgtccc agcgccgaca
      661 cgctgacgcc ttcccgaagg atgtcgccgt tctcaagctg atcgtagacg tcgctgaaca
      721 gcaccagccg gatcgagccg gtgtcgtcct caaggcggaa gcgcacgccg ttctcaaagc
      781 gatcgatgct ttcgatcgtg ccgctgatga acactgtgcg gccgatgtct tccggcgtga
      841 tggcgccgag cggcgccaga cgcaacgtcg ctggttcttc ggccacggcg cgggtgagga
      901 cggacacgtc ggacggaagc tcgggcacga tctccagatc gccccggaac gagttgaccg
      961 gcccttgcgc gacgacttcc gccccgatct gccagtcggc ggcgtccggc agcgcgccga
     1021 atacgtcgtg ccacaacagc agcgtgacgt cgcgaccttg cgggtcgctg agcgtgaact
     1081 tcactccggc cgaaaagggc gcgatcccgg cgatcgtccc ctgcacgcgc acccaccggc
     1141 cgatgtcgtc gtcgccgagg ctgccgatag gctcctgctc ggtaaagacg accggttggt
     1201 ccagcacggc tacgtggtcg ccgcgcgtaa ccgtcacttg cggcgtgcct ttaaagagcg
     1261 tcaccacgcc ggtcgcttcg atcggatcgc cgacccggat gtccggcgcg ggagcgccgg
     1321 ctttcgccgc gtcgcgctcg acggcaaggt cgatctcacc gctcgcgtcg cgcagcgtga
     1381 ccaggcgaag cccgtcgtac ggctggcgca cggcgcgcac ctggccgcgc accgtgaccg
     1441 gggccagcgc gtcgtccggc gtgatcgagc tgatctcgcg cggagaagca gaatcggtga
     1501 cgcgggtaat cgccagcgcg tcgatcgtgc tgagcgtcag cgagggcgtc tcgtcccgca
     1561 cgcgcagggc gccttcgacg accacgcgat cgccgatcga cggcacgcga tcggcctcga
     1621 tcagggcgcg gcctgcgggc cgaaaggccg tgacgagcat ctgcccgctg ccgtcgtcga
     1681 cccagaagga gagcgattcc gcttcagggt tgaccgctgg cgagcgcctg acgacgcctt
     1741 cgatgcggac gtaggcgtag ttcatcgccg cctgaatctg gccgatacgg acggtgggca
     1801 ccgtggcact gacggaggcg acatacagca gcgccaatcc gccggcagcc agcacgatgg
     1861 cgagcaagcc gaacagggcg atcgtcatgc gccgcttgag gttcgctccg caatacggac
     1921 acgtgacgtg tgaaccgact tcgcgcccgc acgacgggca gaaggacaag tcaagtctcc
     1981 agggtttaga cgtggccggt ggtcagtata ttagtggctg ggaccggctg caaggatgtg
     2041 agtgtaagta taaccagatg gggcgggcgc gcaagtggac gagcagtgga aattgcccgt
     2101 tgcaaacgtg gtacaatctg ctcaaatttc acgcagtcgc gcggcgacag acgagcggac
     2161 caatggcttt gcacatatcc gccgaacaga tggcccaata caagcgcacg gctcgcgcac
     2221 gctggcaggc tgagcgggaa cagcaagacg ccagacgcga gcgcgcctgg aaactggccc
     2281 ggcaggcggc agacctgctg aagcgcgagt acggcgtgcg tcgcgtgatg gtcttcggtt
     2341 cgctcacgca ctcggatcgc ttcacgctct ggtcggatgt ggacgtggct gcgtggggac
     2401 tgacaccggc caactggctg agcgctatcg gcgcagtcaa cgcattgtcg agtgaaatcg
     2461 aactgaatgt ggtggatgtg gaatgctgtc cgccgcaact gctcgcagcc atcgagcggg
     2521 aaggagtatc tctgtgatcc cgcgctaccg tctgctggca gaacgggtac gctccgagct
     2581 acgcagccta gagcaggttg tcgaacgggc caataacgcg ttggcacgga caatcccgca
     2641 tccgcaggat caagattact tcctggcggc tgcggcgctt gatctgcacg gcttctacgt
     2701 cgggttggag cgcctgtttg agttaatcgc gaccgagatc gacaggagca ggccgggcgg
     2761 cccaacctgg caccgcgatt tactggcgca gatgtcgctg accgtgcctg atgtgcggcc
     2821 tgcagtcgtt tcggacgaaa cgcgcaccaa gttgaggaat tatctggaat ttcgccacgt
     2881 ggtgcgtaat gtctacacct ttgacttgca gccggatcgg gtaacggaac ttgtaggcgg
     2941 tctgcggcct gctttcgacc tagcccggcg cgatctgctg gccttcgccg atttcctcga
     3001 agagttatcc acagcagacg aagaagaccc tgcgtcttga gcgggcagag cgcctcatgg
     3061 cagtgcgagg atgatcggcc ccagcctcaa ctccgacgcg tagagacacg cctgcgacgc
     3121 tcactttgtg atgaacgggt cgtcgggttc gagcggatgg gttgccttca agcccttcgc
     3181 aaacgctgtc gtgtatggat tcgtcccgtc caacgagttt gccgcgaact cgttcggcca
     3241 gtactgagac ggcaacatct tgggcgtctt gtcgccgctc ttcgggtagt accacgcgcg
     3301 cacggtccag ccacacgaca ggccgacgaa cacggcgcgg actttttggc cggcgctcac
     3361 aggcacggcg ccgtgcagcc aaccctcagg ccggaaccac tggccgaagt ggctgcgcag
     3421 ggtgagcgtg ccggatgcgg gcgctttgtc cgccgtatcc acggtcacgg tgcacttgcc
     3481 gatgcaatac ttgtcggcga ccgtcacatg ctggatcagc agaggtccca cgttgacgta
     3541 ggagctgcca ctcagatact gcaactgcac gtcgatgcgg agggtctcac cttctgatgt
     3601 gccgctctcg ggatacgcca gcggcacggc gagcatgaac gagcctgcgc cgaaagccga
     3661 cgtatacgtc tgcttcaaga gaacgtactg gcccttgttg ttcttgtggt aaacaccgac
     3721 gtcgaggcgg ttgttggaat acgtggacga tccgttgacg acgacggtcg tctcatcctt
     3781 acaggctgcg atgctgtaaa tgccgatcga aacggcgctg gccgccgacg ccggcgccac
     3841 gctgatcagc gcggcaagca gccctaccgc gacgagaaca tgaaagccct tcatgcgaat
     3901 acctcctgaa cgctaccctt gacatccgtt gattcgttgc ctatccgttg acaccttcga
     3961 acaagtcctc ttcgatatga cgaagcccgt tcggcggcgg ttccacgcgt gtgaaccgct
     4021 cgtatcccag tacccggcgt tgcaacacgc cggggaatct tcgaaagagc atactcgact
     4081 cgccctg
//
LOCUS       DBNHXB010000252         5112 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_564901, whole genome shotgun sequence.
ACCESSION   DBNHXB010000252 DBNHXB010000000
VERSION     DBNHXB010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_564901"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1450)
                     /locus_tag="AC162G_10305"
     CDS             complement(104..1450)
                     /locus_tag="AC162G_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5076252.1"
                     /translation="MAAQRTLLKYGCVLSLDPKVGNFKQADVLIEGSKIAAVGPNLSA
                     ADAEVIDASNTIVMPGFVDTHRHIWEGILRNIGTDVPLEGEVSYLAFILNVIAPVYRP
                     EDAYVGDLVGLLGAIDAGITTILDWSHIQATREHADAVIRALQESGMRAVFAYGYPWW
                     KYPDEHQDDWIRDVAKQYFSSKDQLLTFGLASPGPEFIPFEFAKAQWELARELGARIT
                     VHVGVGTAGQHGKLAEMGRAGLLKDDTTYIHCTTLSDDEIQMIVDTGGTFSLASPVEM
                     MMGHGMPPTQRFLDRGLRPSLSVDVETNVPNDMFTQMRSVISLQHALLFDQKLAGKEN
                     VPAPLTTRDLLEFATIEGARANGLADKTGSLTPGKEADVIMLRTDRPNIFPINDPIGA
                     VVWGMDTSNVDSVFVAGKALKRNGELLGVDLNRIRKMAYESRDYVVSKSGFKLPEI"
     gene            complement(1701..2564)
                     /locus_tag="AC162G_10310"
     CDS             complement(1701..2564)
                     /locus_tag="AC162G_10310"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5076253.1"
                     /translation="MHYVSRIGEAAAQTPKLYEKHSAGFRRAAYVDHTIGSVHMGTGI
                     CTLEPGGVIQPHVHSFEESFYILEGNVIAQIGDRGVALGPGHFGLIGTGARHAWRNTG
                     HKAVRWLEMQAPQPRPLDYGRDTFFVDGDVLMQAAPPNLQALDGILIGHFDESQLLRP
                     GGPSQMEGFNPVTGVAIKMFVDRSFGAIHQSLFLIQYQPGAKIDPHDHTFEESYFIVS
                     GRVRATADGKAYDLGPGSVIWTSVGCLHSFANVSDEPVRWIETQAPLPPAREVFRFER
                     DWAGYAANTSV"
     gene            complement(2707..4023)
                     /locus_tag="AC162G_10315"
     CDS             complement(2707..4023)
                     /locus_tag="AC162G_10315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR5076254.1"
                     /translation="MALRTYGTMGVDWEERVNYERLRTERLARIKKLLKQSEIGALLC
                     FDMYNIRYITNTTIGTWAMDKLGRFCLLPQDDDPINWDFGSAARHHALHCPWLGEGRS
                     RAGISTLRGAMPPGAQRAEDVARKIRIELEERGLLHEPLGVDMVEPPVLFALQKEGIQ
                     VVDGQQLMMEARAIKTQDEITLLNMACMMVDAAYEELYRAMKPGIRENECVALVSKVL
                     YELGSEHVEGVNAISGERCSPHPHIFSDRALRPGDPAYFDILHAYMGYRTCYYRTFVI
                     GSASQAQVDAYKRCRYYLDAAIHIIKPGVTTADVAKLWPKAQEFGFPNEEACFALQYG
                     HGVGLAIWEKPVFSRLVSFDSPQVIEEGMVFALETFWPASDGWSAARIEEQLVVTKDG
                     CEVITRFPAEELMIAGVKYYTATGPLPGTRETQSHKNIDYTGVTYQ"
     gene            complement(4044..4418)
                     /locus_tag="AC162G_10320"
     CDS             complement(4044..4418)
                     /locus_tag="AC162G_10320"
                     /inference="COORDINATES: protein motif:HMM:NF046770.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF transmembrane domain-containing protein"
                     /protein_id="HIR5076255.1"
                     /translation="MNENLPPNERPTEVSRQRSSAWLAGAVLIILGVIFLVLNLTGFS
                     IENWWAVFILIPAFGSLAAAWRIYQSSGGRFTPAARGPLIGGLILLTVASVFLFDLDW
                     GAIWPVFLIVIGVAVLLTSLSR"
     gene            4565..>5112
                     /locus_tag="AC162G_10325"
     CDS             4565..>5112
                     /locus_tag="AC162G_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024252.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5076256.1"
                     /translation="MPDTSSHTLNIGEKLRAARKSARMSLRDLADKSEMSASMLSQIE
                     HGKAYPSVRSLYSIAAALSLPVDYFFPTENNREPASTGALPGMAGDMTASELREATIA
                     ATAEGAAVEFHAAPHPSLPVVHRAARPTIELKGGVTWARLTARAEEGVEFLEITYAPG
                     ATSGANMSHHEGREFGLILEGVL"
BASE COUNT          993 a         1590 c         1528 g         1001 t
ORIGIN      
        1 acgccccggt cgtcatcaac ccatattctc ggcccaggcc tcgcgccatt cggtagtcac
       61 aatccccggc atcactgcgt tgacggtgat ctggtcgcct tgcttagatt tcggggagct
      121 taaaccccga cttggacacc acgtaatcgc gagactcgta ggccattttc cgaatacggt
      181 tcaaatccac ccccagcaat tccccgttgc gtttcagggc cttgccggcg acaaagaccg
      241 agtccacgtt gctggtatcc atcccccaga ccactgcgcc gatcggatcg ttgatgggga
      301 agatgttggg gcgatccgtg cgcagcatga taacgtccgc ctctttgccc ggcgtcagtg
      361 agccggtctt gtcggccaac ccattggcgc gcgcgccctc gatggtggcg aactccaaca
      421 gatcgcgggt ggtcaaggga gctggcacgt tctccttgcc cgccagcttc tggtcgaaaa
      481 gcaaagcgtg ctgcagggag atgaccgagc gcatctgcgt gaacatgtcg ttcggcacgt
      541 tggtctcgac atccacactc aagctcgggc gcaggccgcg atccagaaaa cgctgggtgg
      601 gcggcatccc gtgtcccatc atcatctcga ccggggaggc cagcgaaaac gtcccgcccg
      661 tgtccacgat catctggatt tcatcgtcgc tcaacgtcgt gcagtgaatg tacgtcgtgt
      721 catccttgag caggcccgcc ctgcccatct ccgccagctt gccgtgctga ccagcggtgc
      781 caacgcccac gtggacggtg atacgggcgc caagctcgcg cgccagctcc cactgagcct
      841 tagcaaactc aaacgggata aattccggcc cgggagaagc aagtccaaac gtcagcaact
      901 gatctttcga cgagaaatac tgcttggcga catccctgat ccagtcgtcc tggtgctcgt
      961 cgggatactt ccaccatggg taaccatagg caaagacggc gcgcatgccg gactcttgca
     1021 gggcgcggat gacggcgtcg gcgtgctctc gggtggcctg gatatgggac caatcgagga
     1081 tcgtcgtaat cccggcgtca atggcgccca ggagacccac caggtcgccg acgtaggcgt
     1141 cttccgggcg gtagacgggc gcaatgacgt tcagaatgaa cgccagatag ctaacctcac
     1201 cttccagcgg gacatctgtt ccaatgttgc gcagaatgcc ttcccagatg tgccggtgtg
     1261 tatccacgaa gcccggcatg acgatcgtgt tcgaggcgtc gattacctcg gcgtccgccg
     1321 cgctgaggtt cggcccgacg gcggcgatct tggagccttc gatcaacacg tcggcctgct
     1381 tgaagttgcc gaccttcgga tcgagactga ggacacaacc atacttcagc agcgttcgtt
     1441 gggctgccat gtctgcctcc ttggcccttg gcatgggaac ggatttacca gtgattgtgg
     1501 cgaggtcgga taagattggc tgatcgcggc ttcagccttt ggtgtataga ccggatgtgc
     1561 tttcaatttg ctcgataatc gcctccttgt tcagggcgct ctgctgcgct attccagcgc
     1621 cgcccggccc aatccacgcc tgctgcttac agagacctcc atctgccatt tgccatctgc
     1681 tatctgctat ccgccatctg ctataccgac gtgttcgcgg cgtaccccgc ccaatctctt
     1741 tcaaagcgga agacttccct ggcgggcggc agcggcgctt gcgtctcgat ccagcgcacc
     1801 ggctcgtcgc tcacattcgc gaaactatgc aggcagccga cgctcgtcca gatcacgctg
     1861 cccggcccca ggtcatatgc tttaccgtct gccgtcgcgc gcacgcgccc actgacgatg
     1921 aagtaggatt cctcgaaagt gtgatcgtgc ggatcgatct tcgcgccggg ctggtactgg
     1981 atgagaaaca gcgattgatg aatcgcgccg aaggagcgat cgacgaacat cttgatcgcc
     2041 acgccggtga cgggattgaa gccttccatt tgggacggcc cgccggggcg cagcagttgc
     2101 gactcgtcga agtggccgat cagaatgccg tccagggctt gcagattggg tggcgcggcc
     2161 tgcatgagga catcgccgtc gacgaagaac gtatcgcggc cgtagtcgag gggacgcggc
     2221 tggggcgctt gcatctccag ccagcgaacg gccttatggc ccgtattgcg ccaggcgtgc
     2281 cgggcgcccg tgccgatcag cccgaaatga ccaggcccca gcgcaactcc ccgatcgccg
     2341 atctgggcga tgacgttccc ttccaaaata tagaaactct cttcgaacga gtggacgtgc
     2401 ggctggatca cgccgcccgg ctccagagtg caaatgcccg tgcccatgtg caccgagccg
     2461 atcgtgtgat ccacgtacgc ggctcgccgg aaaccagcgc tatgcttctc atacagcttc
     2521 ggcgtctgcg cagccgcttc gccgatcctg gaaacgtaat gcatatctgc cccccagtga
     2581 tcagtgaacg gtgatcagtg aacagtgatc agtgatcggt gaacagtgaa aagtgaacag
     2641 tggtcagtga gcagtgggca ctgcccactg attactgatt actgataact gattactgat
     2701 aactgattac tgataagtca caccggtgta gtcgatgttc ttgtgcgact gtgtttcgcg
     2761 ggtgccgggc agcgggccgg tcgccgtgta gtacttcacg ccggcgatca tcaattcctc
     2821 ggcagggaaa cgcgtgatca cttcgcagcc gtctttcgtg accacgagct gctcttcgat
     2881 gcgggccgcc gaccagccat cggacgccgg ccagaaggtt tcgagcgcga acaccatgcc
     2941 ctcttcgatc acctgtgggc tgtcgaagga taccaggcgg ctgaagaccg gcttctccca
     3001 gatggcgagg ccgacgccgt gtccgtactg caatgcgaag caggcctctt cgttggggaa
     3061 gccgaattcc tgcgctttcg gccacagctt ggccacgtcg gcggtggtca cgccgggctt
     3121 gatgatgtgg atagccgcgt cgaggtagta acggcagcgc ttgtaggcgt ccacctgcgc
     3181 ctgcgaggcc gagccgatga cgaacgtgcg gtagtagcac gtgcgatagc ccatgtacgc
     3241 gtgcagaata tcgaagtagg ccgggtcgcc gggacgcagg gcacgatcgc tgaagatgtg
     3301 cgggtgcggg ctgcaccgct cgccggagat cgcgttgacg ccctccacgt gctccgagcc
     3361 aagctcgtag aggaccttgc tgacgagcgc cacgcactcg ttctcgcgta tgccgggctt
     3421 catcgcgcgg tacagctcct cataagcggc atcgaccatc atgcaggcca tgttgagcaa
     3481 ggtgatctcg tcctgcgtct tgatcgcgcg agcctccatc atcaactgct gcccgtcgac
     3541 gacctggatg ccttcctttt gcagcgcgaa cagcaccggc ggctcgacca tgtccacgcc
     3601 gagcggctcg tggagcaggc cgcgctcttc cagctcgatc cgaatcttgc gggccacgtc
     3661 ctcagcgcgc tgcgcgccgg gcggcatcgc gccgcgcagc gtcgagatgc cggcgcgcga
     3721 tcggccttcg cccagccagg gacaatgcag cgcgtgatgc ctggccgccg agccaaagtc
     3781 ccaattgatc ggatcgtcgt cctgcggcag gagacagaag cgccccagct tgtccatcgc
     3841 ccaggtgccg atcgtggtgt tcgtgatgta gcggatgttg tacatgtcga agcacagcag
     3901 cgcgccgatc tccgactgct tgagcagttt cttgatgcgc gccaagcgct ccgtccgcag
     3961 gcgctcgtag ttgacgcgct cctcccagtc gacgcccatc gtgccgtagg ttcgaagtgc
     4021 catgatgtcc tccttgtagt caactatctg gataacgagg tgagtagcac cgccacgccg
     4081 atgacgatca ggaacaccgg ccagatcgcg ccccaatcca ggtcgaacaa gaatacggat
     4141 gccacggtca gcaggatcaa gccgccgatc agcgggccgc gcgccgcagg ggtaaatcgc
     4201 cctccgctcg actggtaaat tcgccacgcg gcggcaagcg aaccgaaggc ggggatcaat
     4261 atgaagacgg cccaccagtt ttcgatactg aagccggtga ggttcaggac caggaaaatg
     4321 acgccgagaa tgatcagaac ggcgccggcc agccaggccg aactgcgctg ccgggatacc
     4381 tctgtcggtc gttcgttagg cggaaggttt tcattcatag aagtctccta acgcgcgctt
     4441 caattgtctg ctataattta agtgatgtta aacattttaa ccgaaattcc gtcgatttgt
     4501 caagagatac ttaacaaacc ggcgtggaat attcaatcca actgtcatat aatgggtgcg
     4561 cataatgccg gacacatcat ctcacaccct caacattgga gagaagctcc gcgctgcgcg
     4621 caagagcgcc cgcatgagtc tgcgcgacct ggcagacaag tccgaaatga gcgcgagcat
     4681 gctgagccag atcgagcatg gcaaagccta tccgtcggtg cgctctctgt acagcattgc
     4741 cgctgcgctg tccctgccgg tcgattactt ctttcccacc gaaaataacc gcgaacccgc
     4801 cagtaccggc gcgctgcccg gcatggccgg cgacatgacg gctagcgagc tgcgcgaagc
     4861 gaccatcgcg gccacggcgg agggcgctgc cgtcgaattc cacgctgcgc cgcacccgtc
     4921 tttgcccgtc gttcacaggg cggcgcgccc gacgatcgag ttgaaaggcg gcgtcacctg
     4981 ggcgcgactg acggcccgcg ccgaagaagg ggttgagttc ctggagatta cctacgcgcc
     5041 aggggcgact tccggcgcga atatgtctca ccacgagggg cgcgagttcg gattgatcct
     5101 ggaaggcgtg ct
//
LOCUS       DBNHXB010000253        16420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1739206, whole genome shotgun sequence.
ACCESSION   DBNHXB010000253 DBNHXB010000000
VERSION     DBNHXB010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 16420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16420
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1739206"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /locus_tag="AC162G_10330"
     CDS             <1..1451
                     /locus_tag="AC162G_10330"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR5076257.1"
                     /translation="MTLADVIEGLSGARPARSGALQPVRRFVVDSRQAAPRDVFAALR
                     GEETDGHLFVEHAFARGAIAALIDRDVAIGADVVDLRPGRPFPPSVPLPACLRVADVL
                     AALQSLARWWRDRFPALRVIGITGSVGKTSTKELAAAVLSRRYEVLQSEGNLNNEIGL
                     PLTLLELDQHHERVVLEMGMYARGEIAMLCSIARPAVGVITNVGPVHLERLGTIEAVA
                     QAKAELVEALPGDGVAILNYDDARVRPMAGLTKARAFFYGLDPAADLWADDIQSEGLQ
                     GIHFQMHYRDETLHVQVPLLGRHSVHTSLRATALGLVEGMSWDQILEGLQDRRAQLRL
                     VAVPGPNGSTILDDTYNASPESTIAALNLLAELEGRKIAVLGDMLELGSHEEEGHRKV
                     GLRAMDVARVLVTVGERGAIIAEEARRSGMPADRVRVCVSNEEAVEYLKIVIEPGDMI
                     LVKGSRGLHMEDIVAALEPALTLSEGKGKGQG"
     gene            1451..2479
                     /gene="mraY"
                     /locus_tag="AC162G_10335"
     CDS             1451..2479
                     /gene="mraY"
                     /locus_tag="AC162G_10335"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR5076258.1"
                     /translation="MSAALFLGGLSFLLAVIWGAPLIRLLKARGIGKQIRLEGPQTHL
                     VKAGTPTMGGLMIIVPVVLITAALNIANLIGLNQFGQQILVPMGTMMAFGALGAIDDW
                     TNVRGRRVRGEGISGRMKFLIQVILALGIAAFLHFGPTQLRSIAIPGIEQKIDIGLLW
                     IPIAVFIVVGTTNAVNFTDGLDGLAGIINATAFIGYAIIAYWQSFRGEPPFVVSNLVP
                     FCFIVVGAVLAFLWFNAHPAQMFMGDTGALALGATLGVVALMTGQWLLLPVVALIPMA
                     ETLTVILQVAYFKYTKRKYGEGRRLFKMAPLHNHFVLLGWSETQIVQRFWLIGIWSAM
                     VGIALALL"
     gene            2534..3910
                     /gene="murD"
                     /locus_tag="AC162G_10340"
     CDS             2534..3910
                     /gene="murD"
                     /locus_tag="AC162G_10340"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR5076259.1"
                     /translation="MATPVEGRRVVVLGLARQGTALAHWLIGHGARVTVSDVQPADRL
                     RGALKALEGLAIRYELGGHPSSLLVECDLLCLSGGVPIDLPIVQEARKRGIALSNDAQ
                     LFLERCPAAVIGITGSAGKTTTTTLVGDMGRSAGRTTWIGGNIGNPLISDLAAMTRSD
                     LVVMELSSFQLDLMSVSPPVAAVLNVTPNHLDRHGTMAAYVAAKARILDYQSPPDTAV
                     LGYDDPVARSLIGRAKGRLTLFGRSGVGDWADLAHTRVDEGWVVLLKDQRVPICEVSE
                     IRLRGVHNVLNVLAACALADAAGISPQAMRDAISAFAGVEHRLQLVRERSGVQYYDDS
                     IATAPERVIAALHSFQEPIVLLVGGRDKHLPWAEAAQLIVNRVRQLILFGEMAELVQR
                     SIAEVTWRDSLEAVHRVVTLAEAVRKAAEVARPGDVVLLSPGGTSFDAFKDFAERGDK
                     FQEYVRAL"
     gene            4023..5225
                     /gene="ftsW"
                     /locus_tag="AC162G_10345"
     CDS             4023..5225
                     /gene="ftsW"
                     /locus_tag="AC162G_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR5076260.1"
                     /translation="MATQTIRHIKPTSGNSRVPRLDYPLLIVVGVLAAIGLMMVYSTT
                     FDWGYQQFGEPTYFVLRQTLWLLLGIGALWATTRMDYTVLRRFSIPLLGLGLALLVGV
                     LLFGQEVLGAQRAFLNGSIQPSELVKLITVIYIADWLASKGEKIRDVSYGLIPFAVLI
                     GIIAGLIVLQPDFGTTIVIVLVAGMMFFLAGADLLQIVVGLLVSGGTLLALIVNSPYA
                     QERINSYIAGLQDPGQASYQLQQALIALGSGGITGQGPGASHQKFGYLPVPHTDSVFA
                     VLGEELGLIGGLAVLALFAFLAYRGFRIALHAPDSFGMLAAAGVTIWIVTEALINIAV
                     ITGTLPLTGIPLPFISYGGSSLVSLMTAVGLLQSVARSSRKGLTRSALMDSRRRNGRP
                     RLSRVGRR"
     gene            5161..6270
                     /gene="murG"
                     /locus_tag="AC162G_10350"
     CDS             5161..6270
                     /gene="murG"
                     /locus_tag="AC162G_10350"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR5076261.1"
                     /translation="MRLWIAGGGTGGHVYPALAVVEALREAQPNVVTRWIGSRGGVEE
                     ELVARAGIAFDAVPAGGVHGLAPLRAAWNALKLAAGVVRAAGMARAFRPHVLLVTGGF
                     VGVPAAVACWLRRTPILVYLPDIEPGLAVKFASRLAMRVAVSVDDSLQFFPKGARRAI
                     VTGYPIRSELAHATRADAIRRFALEPERKTLLVFGGSKGARSLNRAVASILDTLVARY
                     QIIHISGPTDALEARSRRDTLPDEAKGRYHLFEYLHDEMGLALAAADVAVSRAGASVL
                     GEFPLLGVASILVPYPYAWRYQKVNADYLTSRGAALRLEDENLMRDLLPAIERLMSDD
                     AERRRMQEKARALARPDAASRLAAVLAELSGAVSA"
     gene            6267..6872
                     /locus_tag="AC162G_10355"
     CDS             6267..6872
                     /locus_tag="AC162G_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076262.1"
                     /translation="MIPNPLTLEELTLIVVLSLPLVYWGLQHGLDAAIIAVVSVLAGM
                     ALSDAAASALSGVINIFWRMGKALGGGFGADFYTQFSQESGLITTPEQLEMLGSIIFV
                     VVAFWGLRIARRRAGGRANFLDGLLGALGALVTSYLFVTHLFERHVALPQEIVINPTT
                     ELPGFLAPTGVPTVVLDPNVLVLIVLVIIAYGVQTTKRKSK"
     gene            6879..7466
                     /locus_tag="AC162G_10360"
     CDS             6879..7466
                     /locus_tag="AC162G_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076263.1"
                     /translation="MITLEAYFGGLVGVFAVIGIVRGFLKELGLTTVLLVWLFAMDLI
                     LPRMEEMIEMPDSFMQRLGLTPQTDDMFMWLFFSVLTVIIVFIAYQGETLAFGGTAPK
                     GLLGGLFALLIGLVNGYLVSGTLWWIMNRYNYPGPIQVIPYPGQLLLSPVANNIVNVW
                     KLLPPDLLAGGMDEPGTLGLLPVLLITMILLRVLR"
     gene            7570..8955
                     /gene="murC"
                     /locus_tag="AC162G_10365"
     CDS             7570..8955
                     /gene="murC"
                     /locus_tag="AC162G_10365"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR5076264.1"
                     /translation="MTGKLKSGDRVHIVGIGGAGMSAIATVLLERGHRVSGSDQIESP
                     STQALRERGAQVGIGHRAENVGEVDHVLISSAIKADNVELAAAQRRGIPVSKRAQFLG
                     WLMQGALGVAVAGTHGKTTTTAMIASILARAGRDPSFIVGGTIAGMNTPAHAGRGREF
                     VIEADEYDRTFLGLTPTVAVVTNVEHDHPDCYPTFESMLDAFRAFVGLLPESGFLALC
                     GDDAPARGLMEYRGSRDVVTFGFGSDVTWQATDLRVNNTGGSDFVALRDGATHGLLRL
                     RIPGRHNVLNALAAVAATDFLGVSIEQMREALVEFQGVGRRFELRGEACGVTVVDDYG
                     HHPTEIRATLAAARMAYPGRAIWAVWQPHTFSRTKLLMDEFAAAFEDADHVIVTEVYA
                     SREKDTLGVTAGAVVKRMRHRDTRHAASLLDATNMLAEDVQAGDVVITLSAGDGNVVG
                     DSLLRRLEERC"
     gene            8952..9158
                     /locus_tag="AC162G_10370"
     CDS             8952..9158
                     /locus_tag="AC162G_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076265.1"
                     /translation="MNRCTTHQLPITGMARKPGNAFEATLEKWQRGDDITWGELFAAW
                     LKTPMAPASPDTVRRVIHKVEWLL"
     gene            9146..10108
                     /gene="murB"
                     /locus_tag="AC162G_10375"
     CDS             9146..10108
                     /gene="murB"
                     /locus_tag="AC162G_10375"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR5076266.1"
                     /translation="MAPLTGLRQFLGDRARAGESLSRHTTSRIGGPADLLVEAASADD
                     LRDLVLEARRRNVPVFVLGGGANVLVSDAGMHGLTIVNKARRLEFLDGGRVRAESGVV
                     LPTLARECIARSLSGLEWAVGVPGTVGGAIVGNAGAHGRDTASDVKRAWILGPDSRVR
                     EWTVDELEFEYRSSRIKQEARSRMREPGADTRYALRDTRYVVLAAEFRLARGDRADLE
                     RRAAEFNDYRRRTQPPGASMGSMFKNPSGDAAGRLIDAAGLKRLRVGNAEISSVHANF
                     FVNLGGATARDVMALIERARRTVREQFDVELELEIELVGDWGNG"
     gene            10113..11279
                     /locus_tag="AC162G_10380"
     CDS             10113..11279
                     /locus_tag="AC162G_10380"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR5076267.1"
                     /translation="MKRKTCPERRRRIRVGVIFGGKSGEHEVSLMSAQSVMRAIDKGK
                     YEVVPIGITHEGRWLTGGDPLKALTGGQTSMPQLLESGYVLDADVPRGQTPPEHRELV
                     PGTQAAGIPEVDVVFPVVHGTYGEDGTVQGLLELADIPYVGAGVMASAVAMDKAIMKD
                     VLRSHGLPVVDHVVVKRSSWEADPAPVRAQVAESIGYPCFVKPANLGSSVGVSKAHSE
                     QDLDTAIELAAQYDRKILIEKCVEQAREIEVSVLGNDDPIASVPGEIIPSREFYDYAA
                     KYLDNASELLIPAPLDPETSDQTRQLAIDAYLAFDCAGMARVDFLLSRATGELFIGEV
                     NTIPGFTAISMYPKLWEASGIPYPELIDRLIELALERHAEKQRTRTTYDVERME"
     gene            11276..12103
                     /locus_tag="AC162G_10385"
     CDS             11276..12103
                     /locus_tag="AC162G_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR5076268.1"
                     /translation="MKQRRRPARRPQRGARRYEVVALPTLRGSTRGAYVRHQNLRQAR
                     EFRIDRGRLVSLAVLMTLVLAGVWIGFDETFYVSVPGVTGQTRVPVDDIARASGLIGL
                     HVAWANSAEVESRLLEALPSLRAARVTCSLPADCAITVAEREPFLAWRWGDALVWVDQ
                     DGVVYAVRNGLQDVLTIESSDVQPPPPGQRVDSDLMAAIVAAARALPEVRIFRYSAAR
                     GLEFTDASGYPVYLGTGSNMADRAVVWRALREDLASRGIEPVYIDVRFPLAPYYGRP"
     gene            12159..13400
                     /gene="ftsA"
                     /locus_tag="AC162G_10390"
     CDS             12159..13400
                     /gene="ftsA"
                     /locus_tag="AC162G_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR5076269.1"
                     /translation="MTAQRTIVGIDVGTTKICALVGESGPDGALRIVGVGLSPSRGIK
                     RGVVVNVGEATEAIGAALEKAERSSGYQIARAFVSLAGTHINAVNSRGVVAVVHGERG
                     ISQEDVDRAIEAARAIAIPADREVLHVIPRGFTVDGQDGVRDPIGMTGFRLEVEAHIV
                     TGATSSVHNLLKCIEGANVAVDALVLSSIASGEAVLTETEKDMGVALADIGGGTTDIA
                     IFIDGSVWHTTILGVGGSHITNDVAVGLRLPADAAENVKVAHGHARYQTIDPVETIEV
                     EGFDDGGASRVVRRDVAEIIEARAEEIFNLIMQEVKRSGYDGLLPAGLVLSGGTAQLR
                     GLRELGRQVMNMPVRVGGPHDLLGLVDTISTPAFATSVGLLKWGDRQSDALARTRRSG
                     PSVGRRLGRFLRSLLPDRGGT"
     gene            13548..14666
                     /gene="ftsZ"
                     /locus_tag="AC162G_10395"
     CDS             13548..14666
                     /gene="ftsZ"
                     /locus_tag="AC162G_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR5076270.1"
                     /translation="MMQPQVQPESYAQIRVVGVGGGGTNAVNRMIAEGLQGVDFVAIN
                     TDAQALMLSKAPKRVRIGDKLTRGLGAGGNPDIGERAAEESADDLYEVLRGSDMVFIT
                     SGMGGGTGTGASPIVARIAKEVGALAIGVVTRPFTFEGHKRSASADLGINKLKEHVDT
                     LIAIPNDRLLEIVDKKASLQQAFSVADDVLRQGIQGISELITVPGLINLDFADVKAIM
                     VEGGVALMAVGQASGEDRAMHAAQQAITSRLLDITIGGARGILFNVTGGPNLTLFEVN
                     QAAAIIKETAHPDVNLIFGAVIDENMDDAVRVTVIATGFDQTTSVRRRSIASMQREAL
                     HKPAPAPAQPTVEQTFEFAVREFDRDDLEIPAFLRKRG"
     gene            14986..15435
                     /gene="nrdR"
                     /locus_tag="AC162G_10400"
     CDS             14986..15435
                     /gene="nrdR"
                     /locus_tag="AC162G_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR5076271.1"
                     /translation="MKCPYCGDTDTKVIDTFHESSGGIRRRRECNACGKRFSTVERAI
                     VTTPLVVKSDGRREPFDRQKLSNGIRVACAKRPIPSDALEGLVNNIETRVQQSGKAEV
                     PSKMIGDMVIAGLKELDQVAYIRFAMVYLGLKDVEAVRREIDKILER"
     gene            15627..>16420
                     /locus_tag="AC162G_10405"
     CDS             15627..>16420
                     /locus_tag="AC162G_10405"
                     /inference="COORDINATES: protein motif:HMM:NF014868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HIR5076272.1"
                     /translation="MKRYVRRGPDGKPIETPEGMFRRIARAIAEPEKDHGGDMEATER
                     AFYRLLTSLRFFPNSPTFTGAGTPLGQLAACFVLPIGDDMGRKSDGIFQTLRDAALIQ
                     QTGGGNGFSFSRLRPKGALVKSSAGNATGPVGFLRVYDRAFGEIAQGGSRRGANMSVL
                     RVDHPDIEEFITCKTDENAITNFNISVGITDAFMEAVKRDDGWELRFPDVGAPEYKGF
                     DGTLEQAEAAGIPIKVYKRVRARDLFDKIVGQAHHNGEPGVLFLDAA"
BASE COUNT         2834 a         4818 c         5709 g         3059 t
ORIGIN      
        1 tgatgaccct ggcagatgta atcgaaggct tgagcggcgc gcggcccgcg cgtagcgggg
       61 cgctgcagcc ggtgcggcgt ttcgtcgtcg attcgcgcca ggccgcgccg cgcgacgtgt
      121 tcgctgcgct caggggcgag gagacggacg gccatctgtt cgtggagcac gcgttcgcgc
      181 gcggcgcgat cgccgccctg atcgaccgcg acgtcgccat cggcgcggac gtcgtcgact
      241 tgcgaccggg ccgcccattc ccgccctccg tgccgctgcc cgcgtgcctg cgcgtcgccg
      301 acgtgctggc ggcgctgcag tcactcgcgc gctggtggcg cgatcgcttc cccgccctgc
      361 gcgtcatcgg cattacgggc agtgtgggca aaaccagcac gaaggagttg gcggcagccg
      421 tgctatcccg ccgctatgaa gtactccaga gcgaaggcaa cctgaacaac gagatcgggc
      481 tgccgctgac gctgctggag ctcgatcagc atcacgagcg agtcgttttg gagatgggca
      541 tgtacgcgcg cggcgagatc gccatgctgt gctccatcgc ccggccagcc gtcggcgtca
      601 tcaccaacgt ggggccggtt cacctggagc gcctgggcac catcgaagcg gtggcgcagg
      661 ccaaagccga gctggttgag gcgctgcccg gcgacggcgt tgccatcttg aactacgacg
      721 acgcgcgcgt gcgccccatg gccggcctga ccaaagcgcg tgccttcttt tacgggctgg
      781 atccggctgc cgatctgtgg gccgatgaca ttcagagcga ggggctgcaa ggtatccatt
      841 tccagatgca ctaccgggat gagacgctgc acgtgcaggt gcccctcctg gggcggcaca
      901 gcgtccatac atcgctgcgg gccacggccc taggcctggt cgaaggcatg tcgtgggacc
      961 agattctcga gggcctccaa gatcggcgcg cgcaactgcg actcgtcgcc gtgcccggcc
     1021 ccaacggctc gaccatactg gacgatacgt acaacgccag cccggaatcg accatcgccg
     1081 ccctgaacct gctggccgag ctggaagggc gcaagatcgc cgttctgggt gacatgctgg
     1141 aattaggcag ccacgaagag gaagggcacc ggaaggtggg cttgagggcc atggacgtcg
     1201 cacgcgtgct cgtgacggtg ggcgagcgcg gcgcgatcat cgccgaagag gcgcggcgca
     1261 gcggcatgcc cgcggaccgg gtgcgcgtct gcgtgtcaaa tgaggaggcc gtcgagtatt
     1321 tgaagatcgt gatcgaaccg ggcgatatga tcctcgtgaa aggttcgcgc ggcctgcaca
     1381 tggaggatat cgtcgcggcg ctggagcctg ccctgaccct gagcgaaggg aaggggaagg
     1441 gacagggttg atgtcggcgg ctctgtttct cggcgggttg tcgtttctgc tggccgtgat
     1501 ctggggtgcg cccctcattc ggctgctgaa ggcgcgcggg atcggcaagc agatccggct
     1561 cgaggggccg cagacgcacc tggtgaaagc cggcacgccg accatgggcg gcttgatgat
     1621 catcgtgccc gtcgtgctca ttacggcagc cctgaacatt gccaacttga tcggcctcaa
     1681 ccagttcggc cagcagattc tggtgccaat ggggaccatg atggctttcg gggcgctcgg
     1741 cgcgattgac gattggacaa acgtgcgcgg caggcgcgtg cgcggcgagg gcatctcggg
     1801 ccgcatgaag ttcctcattc aggtcatcct tgccctgggc atcgctgcgt tcctccattt
     1861 cggcccgacg cagttgcgca gcatcgccat tccgggcatc gagcagaaga tcgacatcgg
     1921 cctgctgtgg atcccgattg ccgtgttcat cgtcgtcggc acgacgaatg cggtgaattt
     1981 taccgacgga ttggacgggc tggcgggcat tatcaacgcg acggcgttca tcggatacgc
     2041 gatcatcgcc tactggcaat cgttccgggg agaaccgccg ttcgtcgtgt cgaacctggt
     2101 tccgttctgc ttcatcgtcg tcggcgcggt tctggcgttc ttgtggttca atgcgcaccc
     2161 ggcgcagatg ttcatgggtg acacgggcgc gctggcgctg ggcgcgacgc tgggcgtcgt
     2221 ggccctcatg accggccaat ggctgctcct cccggtggtg gcgctgatcc cgatggccga
     2281 gaccctgacg gtgatcttgc aggtggcgta cttcaaatat acgaagcgca agtacggcga
     2341 gggtcggcgc ttgttcaaga tggcgcctct gcacaaccat ttcgtgttat tgggctggag
     2401 cgagacgcag atcgttcagc ggttctggct gatcggcatc tggtcggcga tggtaggcat
     2461 cgccctggcg ctgttgtaga gagttagaga tgggagttag agtgaggagt tagagtggag
     2521 agttggggtt tcgatggcta ctccggttga gggacggcgc gtcgtcgtgc tggggctggc
     2581 gcggcaagga accgcgctgg cgcactggct gatcgggcat ggcgcgcgtg tcaccgtgag
     2641 cgacgtgcag ccggccgata ggctgcgggg cgcgctcaaa gcgttggagg gtctggcgat
     2701 tcgatatgag ctgggcggcc atccatcctc cctgctcgtc gagtgcgact tgttgtgcct
     2761 gagcggtggt gtgccgatcg acttgcctat cgtgcaggag gcaaggaagc gcggcattgc
     2821 gctctcgaac gatgcgcagt tgttcctcga gcgatgcccg gcggcggtga tcggcattac
     2881 cggctcggca gggaagacga ccaccacgac cctggtcggg gacatggggc gctccgccgg
     2941 gcgaacgaca tggatcggcg gcaatatcgg gaatccactg atcagcgatc tcgcggccat
     3001 gacacgttcg gatctcgtgg tgatggagct ttcgtccttt caattagacc tgatgtccgt
     3061 cagcccgccg gtcgcggcag tgctgaacgt cacgccgaac cacctggatc gacatgggac
     3121 aatggcggct tacgtcgcgg ccaaggcccg cattctggat taccagtcgc cgccagatac
     3181 ggcggtgctg ggatatgatg acccggtagc gcgctcgctg atcggacgag cgaaaggccg
     3241 cctgaccttg tttggccgga gcggagtcgg tgactgggcc gaccttgcgc acactcgggt
     3301 cgatgagggt tgggtggtgc tgctgaagga tcagcgcgtc ccgatatgtg aggtgagcga
     3361 gatccgtctg cgcggcgtgc acaacgtgct caatgtgctg gccgcgtgcg cacttgccga
     3421 cgcagccggc atctcacctc aagccatgcg cgatgcaatc tccgcgttcg ccggcgtgga
     3481 gcaccggctg cagctcgtgc gcgagcggag cggcgtgcag tactacgacg acagcatcgc
     3541 caccgcgccg gagcgagtga tcgcagccct ccactccttc caggaaccca tcgtcctgct
     3601 ggtcggtgga cgggacaagc atctgccatg ggccgaagcg gcgcagctca tcgtcaatcg
     3661 ggtcaggcag ttgatcctgt tcggagagat ggcggagctg gtgcagagat ccattgccga
     3721 ggtgacctgg cgcgacagcc tggaggctgt gcaccgggtc gtgacgctgg ccgaggccgt
     3781 gcggaaggct gccgaggtgg cgcgcccggg cgacgtcgtc ctgctctcgc caggtggcac
     3841 gagcttcgat gccttcaagg acttcgccga gcgcggcgac aagttccagg agtatgtcag
     3901 agcgttataa gatcctatcc gaaaaccact ggactgcgga ctttggtccg cttcagatgc
     3961 ggcaggctca agcctgcact ccagttttcg gatgcgttcc aagtgagggg caggggccga
     4021 tcgtggcgac tcagaccatt cgacacatca aaccgacgtc tggtaatagc cgtgttccga
     4081 gactggacta tccgcttctg atcgtggtag gcgtgctggc ggccatcggg ttgatgatgg
     4141 tctatagcac cacgttcgac tgggggtatc agcagttcgg cgagccgacc tatttcgtct
     4201 tgcgccagac gttgtggctg ttgctcggaa tcggcgcgct gtgggccacc acccgcatgg
     4261 actacaccgt attgcgccgg ttctcgatcc ccctgttggg actcggcctg gcgttgctcg
     4321 tcggggtgct gctgttcggt caggaggtgc tgggcgcgca gagagccttt ttgaacggct
     4381 ccatccagcc gtcggagctg gtgaagctga ttaccgtgat ctacatcgcc gactggctgg
     4441 catccaaggg cgagaagata cgcgacgtca gctacggttt aatccccttc gccgtgctga
     4501 tcggaatcat cgctggattg atcgttctgc agcctgactt tggcacgacg attgtgatcg
     4561 tgctggtggc cggcatgatg tttttcctgg ccggcgcgga tcttctgcag atagtggttg
     4621 gcttgctggt cagcggcgga actctgttag cgctgattgt gaattcgccg tatgcgcagg
     4681 agcgcatcaa ctcctatatt gccggactgc aggatcccgg gcaagccagt tatcagttgc
     4741 agcaggcgct catcgcgctc gggtcgggcg ggataaccgg gcaggggcct ggcgccagcc
     4801 accagaagtt cggttacctg ccggtgccgc acaccgatag tgtgttcgcc gtgctaggcg
     4861 aagagcttgg actgatcggc ggactggccg tgctggcgtt gttcgcgttc ctggcctatc
     4921 gcgggtttcg catcgcgctc cacgcgccgg attcattcgg catgctggcg gcggcgggcg
     4981 tgacgatctg gatcgtgacg gaggcgctga tcaacatcgc cgtcatcacc ggcacactgc
     5041 cgcttaccgg catcccgctg ccgttcatat cctacggagg ctcgtcgctg gtgagcttga
     5101 tgacggcggt tggtttgctg caaagcgttg cgcgcagctc gcgcaaaggg ttgactcgca
     5161 gtgcgcttat ggatagcagg cggcggaacg ggcggccacg tctatcccgc gttggccgtc
     5221 gttgaggcgc tgcgggaggc gcagccgaac gtggtgacgc gctggatcgg cagccgcggc
     5281 ggtgtggagg aggagctggt agcgcgcgcc ggcattgcgt tcgatgcggt tccagccggc
     5341 ggcgtgcacg gactagcgcc tcttcgcgcg gcgtggaacg ccttgaaact ggccgccgga
     5401 gtcgtgcgcg cggcaggcat ggcgcgcgcc ttcaggccgc atgtgctact ggtgacgggt
     5461 ggtttcgtcg gcgtgccggc tgccgttgcc tgctggcttc gacgaacgcc aattctggtg
     5521 tatctgccgg atatcgagcc ggggctggcc gtcaagttcg ccagccggct ggcgatgcgg
     5581 gtggcggtga gcgtcgacga ctctctgcag ttcttcccga agggggcgcg cagggccatc
     5641 gtcaccggct acccgatccg gtctgaactg gcgcacgcca cccgcgccga cgcgatcagg
     5701 cgctttgcgc tggagccgga gcgaaagacg ctgctcgtgt tcggcggcag caaaggcgcg
     5761 cgcagcttga accgcgcggt ggcgtcgata ttggatacgc tggtggcgcg ctaccagatc
     5821 attcacatca gcgggccaac cgatgcgctt gaagcgcgct cgcggcgcga cacgctgccg
     5881 gacgaagcga agggacgtta tcacttgttc gagtacctgc acgacgagat ggggctggcg
     5941 ctggccgcag cggacgtggc ggtgtcgcga gcgggcgcct ccgtgctggg cgagtttccg
     6001 ctgttgggtg tggcgtcgat cctggtgccg tatccatacg cctggcgcta ccagaaggtg
     6061 aacgccgatt atcttacgtc gcgcggcgcg gcgcttcggc tcgaggacga gaatttgatg
     6121 cgcgatttgc tgccggcgat cgagcgcctg atgagcgacg atgcggagcg cagacgcatg
     6181 caagagaaag cgcgcgcgct ggcgcgaccg gatgcggcca gccgtctcgc cgccgtgctc
     6241 gccgagctat cgggggcagt gtctgcatga tcccgaatcc gctaacgctc gaagaactga
     6301 ccctgatcgt tgtgttgtcg ctgcccctgg tttattgggg cttgcagcac ggactggacg
     6361 cggcgatcat cgcggtcgtc agcgtgctcg ccggaatggc actgtccgat gcggcggcca
     6421 gtgctttgtc aggcgtcatt aacattttct ggaggatggg gaaggcgctc ggggggggat
     6481 tcggtgcgga cttctacacg cagttcagcc aggagtcggg cctgatcacg acgccggagc
     6541 agttggagat gctcggctcg atcatctttg tcgtggttgc tttttgggga cttcgcattg
     6601 cccggcggcg cgccggaggg cgcgccaatt tcctcgacgg attgctcggc gcgctgggcg
     6661 cgctggtgac gagttacctg ttcgtcaccc acttgttcga gcggcacgtc gcgctgccgc
     6721 aggagatcgt gatcaatccg acaaccgaat tacctgggtt cttggcgcct accggtgtac
     6781 ccaccgtcgt gctcgacccg aacgtcctcg tcttgatcgt gctggtgatc atcgcctatg
     6841 gcgtgcagac gaccaagcgt aagagcaagt aggtgccgat gataaccctc gaggcgtact
     6901 ttggcggatt ggttggagta tttgccgtca tcggcatcgt ccgcggcttt ctcaaggaat
     6961 tgggcctgac gacggttttg ctggtgtggt tgttcgcgat ggacctgatt cttccacgca
     7021 tggaagagat gatcgagatg cccgattcgt tcatgcagcg cctgggcctc acaccgcaga
     7081 cagacgatat gttcatgtgg ctgtttttct cggtgctcac tgtgatcatc gtgttcatcg
     7141 cctatcaagg cgagacgctg gccttcggcg gcacggcgcc caaaggtttg ttagggggtc
     7201 tgttcgccct cctgatcggt ttggtgaacg gatacctggt gtcgggcacg ctgtggtgga
     7261 tcatgaatcg ctacaactat cccgggccta ttcaggttat cccctacccc ggccaactgc
     7321 tactgtcgcc ggtggcgaac aacatcgtca acgtgtggaa gctgctgccg cccgatctgc
     7381 tggccggggg tatggatgag cctgggacgt tgggcttgct gcccgtgctg ttgatcacga
     7441 tgattttgct gcgcgtgctc cggtagtcgg gtggtcgagt ggtcgagtag tcgagtggtc
     7501 gggtagtcca gtggtgcgtg ttgcgtgttg gctgatgact gataactgat aactgataac
     7561 cgatgactga tgactggtaa gttgaagtcg ggcgatcggg ttcatattgt cggcatcggc
     7621 ggcgcgggga tgtcggccat cgcgacggtg ctgctcgaac gcggacaccg tgtcagcgga
     7681 tcggatcaga tcgagtcgcc ctcgacgcag gcgctgcggg agcgcggcgc gcaggttggc
     7741 atcggccacc gggccgagaa cgtgggtgag gtggatcacg tgcttatctc atctgccatc
     7801 aaagcggaca acgtggaact ggccgcggcg cagcgacgcg gcatccctgt ctccaagcga
     7861 gcgcagttcc tgggctggct catgcagggc gcgctcggcg tggctgtggc tggcacgcac
     7921 ggcaagacga cgaccacggc gatgattgcc tcaatcctgg cccgcgccgg ccgcgatccg
     7981 agcttcattg tgggtggcac gattgcaggg atgaatacgc cggcgcacgc cggccgcggc
     8041 cgtgagttcg tcatcgaagc tgacgaatac gatcgcacgt tcctgggatt gacgccgacg
     8101 gtggccgtgg tgacgaatgt cgagcacgat catccggact gctatccgac cttcgagtcg
     8161 atgctcgacg cattccgcgc gttcgtcggg ctgctgccgg agagcggttt tttggcgctg
     8221 tgcggcgatg acgcccccgc tcgcggcctg atggagtacc gcggcagccg tgacgtcgtc
     8281 accttcggct ttgggagcga tgtgacctgg caggcgaccg atctgcgcgt caacaatacc
     8341 ggcggctccg acttcgtggc gctgcgcgac ggcgcgacac atgggctgct gcggctgcgc
     8401 attccaggcc gccacaacgt gctgaacgcg ctggccgccg tcgcggcgac agactttctg
     8461 ggcgtgtcga tcgaacagat gcgcgaagcg ttggtggagt tccaaggcgt gggacgtcgc
     8521 ttcgaattgc gcggcgaggc gtgcggcgtg accgtcgtcg acgactacgg ccatcacccg
     8581 acggagatcc gcgcaacgct cgccgcagcg cgcatggcgt atcccggacg cgccatctgg
     8641 gcggtgtggc agccgcatac gttcagccgc acgaagctgt tgatggacga atttgcagcc
     8701 gcgttcgagg atgcggatca cgtgatcgtg acggaggtat acgcttcgcg cgagaaagac
     8761 acgctgggcg tcacggccgg cgccgtcgtg aaacgcatgc ggcatcgtga cacccggcac
     8821 gcggcttcgc tgctcgacgc gacgaacatg ctggcggaag acgtgcaggc cggcgacgtc
     8881 gtcatcacgt tgagcgcggg cgacgggaac gtggtcggcg atagcttgct gcgccggctc
     8941 gaggagcgct gttgaatcga tgcaccactc accaattacc aattactggc atggcgcgca
     9001 agccgggtaa tgccttcgag gcgacgctcg agaagtggca gcgcggcgac gacatcacat
     9061 ggggcgagtt gttcgcagcg tggctgaaga cgccgatggc gcctgcctcg cccgacacgg
     9121 tgcggagggt gatccacaaa gtcgaatggc tcctctaacg ggtttgaggc agttcctcgg
     9181 cgatcgggcg cgagccggcg aatcgttgag tcggcatacg acctcgcgca tcggcggccc
     9241 ggccgacctg ctggtggagg cggcgagcgc cgacgatctg cgcgatctgg ttctcgaagc
     9301 gcgaagacgc aacgtgccgg ttttcgtgct cggcggcggg gcgaatgtcc tggtcagcga
     9361 tgcgggcatg cacggactga cgatcgttaa caaggctcga cgcctggaat tcctggatgg
     9421 aggccgcgtg cgagcggaga gcggcgtggt cctgccgacg ctggcgcgcg agtgcatcgc
     9481 tcgcagcttg agcggtttgg agtgggccgt gggcgtgccc ggcacggtgg gcggcgcgat
     9541 tgtcgggaac gccggcgcgc acggccgcga cacggcgagt gacgtgaagc gggcatggat
     9601 tctcggcccg gatagccgtg tgcgcgagtg gacagttgac gagttggagt ttgagtatcg
     9661 cagcagccgg attaagcaag aagcaagaag caggatgcgg gagcctgggg ccgatacgcg
     9721 atacgcgcta cgcgatacgc gctatgtggt cctggcggcc gagttccggt tggcgcgcgg
     9781 cgatcgcgct gacctggaac gcagggcggc ggaattcaac gactaccggc ggcgcacgca
     9841 accgccgggg gcgagcatgg ggtcgatgtt caagaatcca tcgggcgatg cggcaggtcg
     9901 cctgatcgac gcggctggct tgaaacgcct gcgcgtcggc aacgcagaga tttcgagcgt
     9961 gcatgcgaac ttctttgtga atctcggcgg cgcgacggca cgggatgtga tggcgctcat
    10021 cgagcgggcg cgccgaacgg tgcgtgagca attcgacgtc gagttagagc tagagattga
    10081 gctggtcggc gattggggca acggatgaga acatgaagcg caaaacctgt cctgagcgta
    10141 ggcgaaggat acgcgtgggc gtgatcttcg ggggcaaatc gggcgagcat gaggtgtcgc
    10201 tcatgtcggc ccagtcggtc atgcgcgcga tcgataaagg caaatacgag gtggtgccta
    10261 tcggcattac ccacgagggc cgctggctga cgggcggcga tccgctcaag gcgctgaccg
    10321 gcgggcagac ctcgatgcct caactgctgg agagcggcta tgtgctcgac gcggatgtgc
    10381 ctcgcggcca gacgccgcct gagcatcgcg agctggtgcc cggcacccag gctgccggca
    10441 ttcccgaagt cgacgtcgtg tttccggtgg tgcacgggac gtacggcgaa gatggcacgg
    10501 tgcaagggtt gctcgagctg gccgacatcc cttacgtcgg cgcgggcgtt atggcgagcg
    10561 cggtcgccat ggataaggcc atcatgaaag acgtgctgcg cagccacggg ctgccggtcg
    10621 tggatcacgt cgtcgtgaag cgatcgagtt gggaggccga tccggcgccg gttcgagcgc
    10681 aggtggctga atcgatcggg tatccgtgct tcgtgaagcc ggcgaatctg ggatcgagcg
    10741 tgggcgtcag caaggctcac tcagaacagg atctggacac ggcgatcgaa ctggctgctc
    10801 agtacgatcg gaagattctg atcgagaaat gcgtggagca ggcgcgcgag atcgaggtca
    10861 gtgtgctcgg caacgacgat ccgatcgcgt cggtgcccgg tgagatcatc ccgagccgcg
    10921 agttctacga ttacgcggcc aagtatctcg acaacgcgag tgaactgctc atccctgcgc
    10981 ccctggatcc ggaaacttcc gaccaaacac ggcaactggc gatcgatgcg tacctggcgt
    11041 tcgactgcgc gggcatggcg cgcgtcgatt ttctgctatc gcgagcgacc ggagagttat
    11101 ttatcggcga ggtcaacaca atccccggtt tcactgcgat cagcatgtat ccgaagctat
    11161 gggaagccag cggcattccg tacccggaac ttatcgaccg tctgatcgag ctggcactgg
    11221 agcgccacgc cgaaaagcag cggacacgca cgacgtacga cgtggagcgc atggaatgaa
    11281 gcagcgcagg cgaccggccc gccggcctca acgcggagcg cggcgctacg aagtcgtagc
    11341 gctgcccacg ctgcgcggct cgacgcgcgg agcctatgtc cggcatcaga atctgcggca
    11401 ggcccgtgag ttcagaatcg atcgcgggcg gctggtgagc ttggctgtgc tcatgactct
    11461 cgtgctggcc ggggtgtgga tcggattcga cgagacattc tacgtatcgg tgccgggtgt
    11521 gacaggccag acgcgcgtgc cggtggacga tatcgcccgt gcgagcggcc tgatcggact
    11581 gcatgtcgca tgggcgaact cggccgaagt cgaatccagg ctgctcgagg ctctgccgtc
    11641 gctgcgcgcc gcgcgggtga cctgctccct gccggcggac tgcgccatca cggtcgcgga
    11701 gcgcgagcct tttctcgcct ggcgatgggg cgacgccctg gtatgggtgg accaagatgg
    11761 cgtcgtctat gcagtgcgca acgggttgca ggatgtgctg acgatcgagt cgagcgatgt
    11821 tcaaccaccc ccgccgggcc agcgtgtcga ttccgatctg atggcggcaa tcgtggctgc
    11881 cgcgcgggcg ctgccggagg tgcgcatctt tcggtactcg gccgcgcgcg gcctggaatt
    11941 caccgatgcg agcggttatc cggtgtactt aggcaccgga tctaacatgg cggatcgggc
    12001 ggtggtctgg cgcgcgcttc gcgaagacct ggcttcgcgc ggaatcgagc cggtttatat
    12061 cgacgtgagg ttcccactgg cgccgtacta cggccgcccg tgacaaagga agattgcctg
    12121 ccgcttgcgc cccgcccagc ggggtcaagg acttggatat gacagcccaa cgcaccatcg
    12181 tcggaatcga cgtaggcacc acgaagatct gcgcgctcgt cggcgaaagt ggccccgatg
    12241 gtgccctgcg catcgtcggc gtggggctgt cgccgtcgcg cggcatcaag cgcggcgtgg
    12301 tcgtcaacgt cggcgaggcg actgaagcca tcggcgcggc gctggagaag gcagagcgta
    12361 gcagcggata ccagattgcg cgggcgttcg tcagcctggc cgggacacac atcaacgcgg
    12421 tgaacagtcg aggcgtggtg gccgtcgtcc acggcgagcg cggcatttcg caagaagacg
    12481 tcgaccgcgc catcgaagcg gctcgcgcga tcgccatccc ggccgatcgc gaggtgctgc
    12541 acgtgatccc gcgcggcttc acggtcgatg ggcaggacgg cgtgcgcgat ccgatcggaa
    12601 tgaccggttt ccgattggaa gtcgaggcgc acatcgtaac cggcgcaacg tcgtcggttc
    12661 acaacttgtt gaagtgcatc gagggcgcga acgtcgccgt ggatgcgctg gtgctcagtt
    12721 cgatcgcttc gggcgaggcg gtcctcaccg agaccgagaa agacatgggc gtggcgctgg
    12781 ccgacatcgg cggcggcacg acggacattg cgatctttat cgacggtagc gtctggcaca
    12841 cgacaatcct cggcgtcggc ggcagccaca tcaccaatga cgtggcggtg gggctgcgcc
    12901 ttcctgcgga cgcggccgag aacgtcaagg tcgcgcatgg gcatgcccgg taccagacga
    12961 tcgatccggt ggagacgatc gaagtagagg gcttcgacga cggcggcgcg tcgcgcgtgg
    13021 tgcgtcgcga cgtcgccgaa atcattgaag cgcgggcgga agagatcttc aacctcatca
    13081 tgcaggaagt caagcgctcc ggctacgacg ggcttttgcc ggcgggtctg gtgctcagcg
    13141 gcgggacggc gcagttgcgc ggcctgcgcg agctgggccg gcaggtcatg aatatgccgg
    13201 tgcgcgtggg cgggccgcac gacctgctcg gcctggtcga taccatttcg actccggcgt
    13261 ttgcgacgag cgtcggcctc ttgaagtggg gcgatcgcca gagcgatgct ctggcgcgca
    13321 cgaggcgcag cggccccagc gtcggccggc gactgggtag gttcctccgc tcgctgctgc
    13381 ccgatcgagg tggaacatga cacgctgctt gcgccccgcc cagcggggtg acacgcacga
    13441 acctgaacgg ccaccttgaa aggaggcggc agcctatgga ctcgatcgca cagccggata
    13501 atcaagaacc aatacacgct cagcaagaaa tagatcaaga ggagatgatc atgcaaccgc
    13561 aagttcaacc ggagtcatat gcacagattc gtgtggtcgg cgttggcggc ggtgggacga
    13621 acgccgtcaa ccggatgatc gccgaaggcc tgcagggtgt ggacttcgtc gccatcaaca
    13681 ccgacgcgca ggcgttgatg ctgtcaaaag cgccgaagcg agtgcgcatt ggcgacaagc
    13741 tgacgcgcgg gctgggtgcg ggaggcaacc cggacatcgg cgagagggcc gccgaagaga
    13801 gcgcggacga tctgtacgaa gtgctcagag gctcggacat ggtgttcatc acgagcggca
    13861 tgggcggtgg cacgggcacg ggcgccagcc cgatcgtggc ccgcattgcg aaggaagtcg
    13921 gcgcgctggc gatcggcgta gtgacgcgcc cgttcacatt cgagggccac aaacgctcgg
    13981 cttcggcgga tctgggaatc aacaagctga aagagcacgt cgatacactg atcgccattc
    14041 ccaacgaccg tctgctggag atcgtcgaca agaaagcgtc gctgcagcag gccttcagcg
    14101 tggccgatga cgtgctgcgc cagggcattc agggcatcag cgagctcatc acggtgccgg
    14161 gcctgatcaa cctcgacttc gccgacgtga aggcgattat ggtggagggc ggcgtggcgc
    14221 tgatggccgt cggccaggcg tcgggcgagg atcgcgcgat gcacgcggct cagcaggcga
    14281 ttaccagccg gctgctcgac atcaccatcg gcggcgcgcg aggcattctg ttcaacgtca
    14341 ccggcggccc gaacctgacc ctgttcgaag tcaaccaggc ggccgccatc atcaaggaga
    14401 ccgcgcatcc cgacgtgaac ctgatcttcg gcgcggtgat cgacgagaac atggacgacg
    14461 cagtgcgcgt caccgtgatc gccacgggct tcgatcagac gacttccgtc cggcgccgct
    14521 cgatcgccag tatgcagcgc gaggcgctcc acaagccggc gccggcgccc gcccagccga
    14581 cggtcgagca aaccttcgaa ttcgccgtgc gcgagttcga ccgggacgat ctggagattc
    14641 cggcgttcct gcgcaagcgc ggctgacctc gccctcttgc aggccccctc tcctgaagaa
    14701 tgctctcgcc attgggggag ggggccgatt ccttgtcctt cactccgtgt tccctctggc
    14761 acggttcggt ttggcgtcgt tcaacccagc gcaatacagt tagaacacat gttttttaag
    14821 gtttggctat tgctttcggc aagtcactgt gctataatgc cgaggcttag cacccatatg
    14881 ttggggtcga gcggtacttt tttcccatat atgtgcaatc cgtcatggcg cggatcgtgg
    14941 ctgacataac ttcgaaatag cacgctccct gttgcgaggc agctcgtgaa gtgcccatac
    15001 tgcggtgaca ccgacaccaa agtcatcgat acttttcacg aaagcagcgg cggcattcga
    15061 cgccggcgcg agtgcaacgc gtgcggcaag cgtttttcga ctgtcgagcg cgcaatcgtt
    15121 acgactcccc tggtcgtgaa gagcgatggc cgccgcgagc cgttcgatcg ccagaagctc
    15181 tcgaacggta tccgcgtcgc ctgcgccaag cgcccgatcc cgagcgatgc gctcgaaggg
    15241 ttggttaaca acatcgagac gcgcgttcag caatcgggga aagccgaagt gccgagcaaa
    15301 atgatcggcg acatggttat tgccggcttg aaagagctgg atcaagtcgc ttacatacga
    15361 ttcgctatgg tgtatctcgg cctgaaggac gtggaggcgg tccgccgcga aatcgacaag
    15421 attctcgagc gctgaaccca gacgccggac gcggataacc ttaccggctt cgcgtccggc
    15481 gtctgcgttt gttggtgtgt ggttggtagt catcacgtgt gtgaaggaga cacgtcatgt
    15541 tggaaggcac gcgtttagag agggcagact ctgggcaggc tgacgaatct gaattgcaat
    15601 tgactccgaa tgcgcggacg gtgctcatga agcgctacgt gcggcgcggg ccggacggca
    15661 agccgatcga gacgccggaa ggcatgttcc gtcgcatcgc gcgcgccatc gccgagccgg
    15721 agaaagacca cggcggcgat atggaggcca ccgagcgcgc gttctatcgc ttgctgacct
    15781 cgctgcggtt ctttcccaat tcgcccacgt tcaccggcgc gggcacgccg ctcgggcagc
    15841 ttgcggcatg cttcgttctc ccgatcggcg acgacatggg ccgcaagagc gacggcatct
    15901 tccagacgct gcgcgacgcg gcgctcattc agcagaccgg aggcggcaac ggcttctcgt
    15961 tttcgcggct gcgcccgaag ggtgcgctgg tcaaatcgtc ggcgggcaat gccaccgggc
    16021 cggtcggttt cctgcgcgta tatgacaggg cgttcgggga gatcgcgcaa ggcggaagcc
    16081 gcaggggcgc gaatatgagt gtgctgcgcg tcgatcatcc cgatatcgaa gagttcatca
    16141 cctgcaagac agatgaaaac gctatcacca atttcaacat ctcggtcggc atcaccgacg
    16201 ccttcatgga agcggtcaag cgcgacgacg ggtgggaact gcgcttcccc gacgtgggtg
    16261 cgcccgagta caagggcttc gacggcacgc tggagcaggc cgaggcggcg ggcatcccga
    16321 tcaaagtcta taagcgcgtg cgcgcacgcg atctgttcga caagatcgtc gggcaggcgc
    16381 atcacaacgg cgagccgggc gtgctgtttc tcgacgcggc
//
LOCUS       DBNHXB010000254        24407 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2746988, whole genome shotgun sequence.
ACCESSION   DBNHXB010000254 DBNHXB010000000
VERSION     DBNHXB010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 24407)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24407)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24407
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2746988"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..4649
                     /locus_tag="AC162G_10410"
     CDS             18..4649
                     /locus_tag="AC162G_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5076273.1"
                     /translation="MSRAVTSVLDPDDVIRRVAQLITERFGFYYAAVFLLDDLGRFAV
                     LREAIGAAGSILRASQHRLEIGGQSMVGAAIASRQPHVARNVGDETMRFANPLLPDTQ
                     SEVALPLLVGDRVLGALDVQSRQLDAFDENSLAMLQSLADQIAIALNNAQQYQEAQLD
                     ARQATALFEASRLVGFISEDLSAAADHLLRTVAEQAGFDSWTAAAYEADSATYTLLCA
                     FQDPARRLSAEHRHDADFDTDTRIGGMDAPNALAIRMRQAVVINDPEIDPLLSQALPA
                     EREAYGRSVSVPAVLGDRVVGAISLGRAIDKRPISARDVQLAQAIANQLAVTIENRRL
                     FEQSRKAVAELNSLMQRYTHEGWARFSQADHATVEHEFRRPAPALSRSHSRSRERSEG
                     AGEGSRVAPSSEDARNARGDSGHRPPQTAGEGRQPALAVPIELRGEVIGMLDVQAEGD
                     HQWSQDELVMLKSVADQVAQSVEAARLLDETEASLQETVKLYEASRAMAAAATPAEVM
                     HTVVSVLHGYLEVDQVSLVLFDEEQEYGRVAAQAVPSPGAETIRVPMAENPGALAMLA
                     DGRSIAISHLTGHPVAAPVADLLRQMGALGESFLVLPLVVGQRLIGFVGASSCGRPTP
                     REFRDNEIAFCETLSRQAAVALENRRLFEETRYRAEIESTLNRISQTIKASLDPQTVL
                     DLQTTIAEMGRAMRASRSFYLANVDDRRFVVAYEWREAADNAASLRGVTGVWMDAPNT
                     FQAFKAGEPLLVSSVERQGVPGLRPPLLAKDALSHMHVPVVSGSRLIGVLGFDQVERE
                     RRWLPEEVSMVRRVADQLAAALENARLYQDVRARVNELTALTRIGRRLAASLQLEEVL
                     NTIVEEAINITPADRGSIALYDPIRDALELRVLIGFPEAALRTIRDNTYTVLQRGDGL
                     HGRLLTTGQAVLVNDVLSDPAYIIADSDTRAELIVPIRQGETLLGALNLESPHAYAFG
                     EPDQRLIEALADQAAVAIANARAYEAERAALDRMREVDRLKTQFLANMSHELRTPLNS
                     IIGFSRVILRGIDGPLTEMQQADLTAIHNSGQHLLGLINDVLDLSKIEAGKMELASED
                     IDLAEVVRGVMSTAIALIKDRKIELHQEIPPDLPLVRADPRRIRQVVLNLVSNAAKFT
                     ETGRITTRVIVGTTEVWVSVVDTGIGIQPDKLEHIFEEFTQVDPSTTRKAGGTGLGLA
                     ISRKFVEMHGGRIWAESQMGVGSTFTFTLPLARPALHTDSDTDDTDTTLPLPEPGRPL
                     ILCIDDDPEVITLYRRFLEKQGYQVIGLRDTERAVQEAARLKPATIILDVLMPAERRD
                     GWSILAELKSTPATRDIPIVVCTIVEDQNKGFALGAADYLVKPVSESDLMRALDRASF
                     SKAIHTVLVVDDELDAIRLLRRMLEGRPGVNVIEAQGGAMGIAAAQTYQPDLVLLDLM
                     MPDIDGFAVLETIRGNRHTHDIPVIVVTAKDVTEEDRARLHGKVVAMLNKGALDSERL
                     MNEIAAALARLNRRQPAPALPRETGERPGARGAQRDLPIENHVKSERPL"
     gene            4646..6373
                     /locus_tag="AC162G_10415"
     CDS             4646..6373
                     /locus_tag="AC162G_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR5076274.1"
                     /translation="MMLDRRLRFDPVLECLRCGTRLPVPVAQAQVGEAYQPLPAACPN
                     CDFTMLDVRYDYDALRDSLPGLLRDRMFNMWRYLELLPVRDPASIVTMGEGGSPLIRL
                     ANLGVMLGRSRIFIKDERQGPTGSFKDRQASLAISVMKESGILECVVASTGNVAISYS
                     AYCARAGIKLWAFLTSMVPAEKMREVALYGSEVVKVTGTYDQAKEVAATFAQHRGLYL
                     DRGIRSIAARESMKTLAYEVSEQLALVDHSVHNGRRPLFRAPDWYIQSVSGGLGPLGA
                     YKGFGELKRMGFTDRIPKFALIQVEGCAPMSRAFKAGKEVADVVSDPQTHIATLSTGN
                     PGPAYALLRSMILEHDGTMDMVSDEDAFRAMHITAKMEGISVEPAAAVAFAGLFKLIE
                     AGLIRPDEDVVVNCTGHTLPVTQELLGDEWALSFEMPTAPGPAAPLPQEGLLSALERL
                     DKRVNRILIVDDNADARRLLRRIMQARGRYTLFEAADGRQALAMAAGERPDLILLDLM
                     MPEMDGFQVLEALKQSESLRQIPVIVVTAKELTPLERQRLAGQADSLLQKGSFTDLDS
                     LDDLVDALI"
     gene            6417..7751
                     /locus_tag="AC162G_10420"
     CDS             6417..7751
                     /locus_tag="AC162G_10420"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR5076275.1"
                     /translation="MDATDHQKRVLYIEDDPGSRLLVKRILESEGYVVDEAADGLSGI
                     SSAQKNLPDLILVDVNLGEVSGHDVATKLKGMPETARIPLVALTAATISGDRERALAA
                     GCDGYIPKPIDVDRLPNLVRRFLGGARERVADSVRAERLEEYSRTLVDRLQATVADLQ
                     KANAELRRLDKMKSDFVVLASHELRTPLTLVYGYVNLLKLEIGRLPDNAHLLDVTQRL
                     SDATTRLTELYDAIVNVSLIDTQRLNVTLVPIDLFGLIASVVSELSPVARQRSLRLNV
                     ADFRGLPKILADADYLRRAISNVAGNAVKYTPDGGTIGLSYQLQPDAIDIVVTDSGIG
                     IDPAEHERIFQKFAVTEDIRYHSTSRTAFQGGGLGLGLAVVRGIVEAHGGRVWVESEG
                     HDPARLPGTTFHILLPASGRRRPESPPTKSPPTESPPTESPPTADSVRGAAV"
     gene            7748..8686
                     /locus_tag="AC162G_10425"
     CDS             7748..8686
                     /locus_tag="AC162G_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5076276.1"
                     /translation="MTSRRRAQQRGIAAALLSAVFLGTAPIFGKQAYAAEMTPIALAA
                     WRILIAAAGLWLVYALIGRRYLYIYPAGLIGCVAVGAVNGVGSLMYYSGLERVDAGVG
                     QMIYSLYPLFLVMFLRLDGHRFSGLTLFRMVLAVVAIALITQAGGGPVDVVGLGLMLG
                     AGFGYALHLAIGQRVSYEMPAQTLTLYAVTSMAVVVCAASLIAGWSPVPAGAWPPTLG
                     LAWVTVASRLGMFLGVKRLGSLQTALIGITEILVTLILALTLLGETLSSRQWIGAGLL
                     IASLLLIGREPTLGGALPRKIQSAASRSSSIAAESQ"
     gene            complement(8574..8975)
                     /locus_tag="AC162G_10430"
     CDS             complement(8574..8975)
                     /locus_tag="AC162G_10430"
                     /inference="COORDINATES: protein motif:HMM:NF016001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HIR5076277.1"
                     /translation="MANSQARERGWRLAIKQIAATRAASWLSSHILHHVDRPLLRLSN
                     GRYSATGILTGLPVVSLTTTGARSRLPRSLPLVGLEDGDKVVLIASNLNGIGFTAIRP
                     LSTRSARRQIGSCAGAPRPTSARGRSGASWR"
     gene            complement(8980..10089)
                     /locus_tag="AC162G_10435"
     CDS             complement(8980..10089)
                     /locus_tag="AC162G_10435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5076278.1"
                     /translation="MSDSPQVSTDAVARQRDAFVERFLHFASGTFNIFTIYIGDRLGL
                     YRALAQGGPSTAAELATRTSMQERYVREWLEQQTVAGILEVEDENREAAARRFRLPPG
                     HVEVLIDCDSLNYLAPLVQLIVGAVHPIEAVLDVYRNGGGVPFSAYGANLREGQAAIN
                     CPAFTRQLAQEWLPAMPDVHARLQADPPARVADIGCGYGWSSIGIAQGYPRVLVDGFD
                     LDAPSIERARLVARAWGLTDRVHFQVRDAGDPALEGAYDLVTAVECVHDMSDPVGALR
                     TMRRLSGERGTVLIVDERVGESFTAKGNDVEWMMYGWSVLHCLPVGMADRPSAGTGTV
                     MRADTLRRYAAEAGFGSVEILPVDNFFFRFYRLNA"
     gene            complement(10200..10916)
                     /locus_tag="AC162G_10440"
     CDS             complement(10200..10916)
                     /locus_tag="AC162G_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5076279.1"
                     /translation="MPPEAKHTILVVDDEPRMIQFIRMNLELEGYRVVQATNGLEALN
                     QVRDELPDLVVLDVMMPDLDGFETLRLVRETSTVPVIMLTVKDDEDDKVKGLELGADD
                     YVTKPFSPRELTSRIKAALRRAELPGLVEKSTIRVDDYLQIDFANRRVIAGGKEVKLR
                     PTEYRLLYHLVQNAGHIVPHDTLLSKVWGYEYRDETHYLRLYITYLRQKLEPDPANPR
                     YIFTERGVGYRFVDFKSGER"
     gene            complement(10970..11212)
                     /locus_tag="AC162G_10445"
     CDS             complement(10970..11212)
                     /locus_tag="AC162G_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076280.1"
                     /translation="MPYTILIHLHNEDPVVGEVDELPHPTDNFVLVTNVRRRDGKDVL
                     FIDPSAASVIFPWTRIAFIEVMTTEEEGEVIGFVRE"
     gene            complement(11249..11758)
                     /locus_tag="AC162G_10450"
     CDS             complement(11249..11758)
                     /locus_tag="AC162G_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012708.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR5076281.1"
                     /translation="MIECPSCGRQHRPGTLFCSECGVYLLTGGPLRTEPLPESELPTA
                     RADPWASSSGAQTAESEQTTLTVVMSATGRRVALPLGGEAVLGRLDATRGVFPDVDLT
                     PDGGLEGGVSRRHARVHQQKSQFFIEDLGSANGTFLNGQRLTPYLPHPLRDGDEVQLG
                     RVRLRISVQ"
     gene            complement(11755..13239)
                     /locus_tag="AC162G_10455"
     CDS             complement(11755..13239)
                     /locus_tag="AC162G_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR5076282.1"
                     /translation="MIEEPDFYEILGVPQNASLDDIKRAYRVLAKQFHPDARETPAGT
                     SMLFRQVQEAYEVLSDDQRRTAYDRQLGLAGRNFDALFTLDVQLSRTILQVIPEEQIL
                     YALFEVKATRAMSRVQRLPLNLCLVIDRSTSMQGVRLDQVKAAAHQLIDSLQEGDALA
                     VVAFSDRADVVWPGQTVGDTMRAKSRVTGIQAAGGTEILQGLKLGMGELERHRRLQSV
                     NHLILLTDGQTYGDEEKCLELALEAKRRSVGISAMGIGEDWNDQLLDAIASRSGGASH
                     YIASAADVPAILNERLKSLATVYGDDLRLTPRLSDNVRIRGAFRLTPYIQRMDMDGDA
                     LLLGTLQADASIAGLFELVVGQREPGVRRLAQLELTGNVPALERTDERVQRNISATFT
                     TEPPPGQAVPPHLLNALAKVTIFQMLESAWTSLEKGDVAAATQRLETMATRLLDVGEH
                     QLARATLLEAGRLARSGHLSPGGQKTIKYGTRGLSLAAMKDQKS"
     gene            complement(13525..14343)
                     /locus_tag="AC162G_10460"
     CDS             complement(13525..14343)
                     /locus_tag="AC162G_10460"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR5076283.1"
                     /translation="MDHRSPRGAAPAAARAPHLRAGSATHVGRVRTHNEDVLFTFTSE
                     LQGDAALDAFGLFILADGMGGHQAGEVASSLAVRTVAREVLEHVYTPYLIHGRPGTTQ
                     TPLAEALTQAVDEASKMVYNNVPGGGTTLTCLLVVNERAVIAHVGDSRAYVYHDGVLH
                     QITRDHSYVDKLVELGQLTAEEAAVHPQKNVLYRAVGQEGLLEVDTHVETIPRGGCLL
                     LCSDGLWGMLSDEEIARIVSHAATAQAACNQLVDAANEAGGRDNITAIVVEVDA"
     gene            complement(14407..16278)
                     /locus_tag="AC162G_10465"
     CDS             complement(14407..16278)
                     /locus_tag="AC162G_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR5076284.1"
                     /translation="MSSLDTRHIGTEQEPTAGGTLAPGRVLQSRYEIRSVLGMGGFSA
                     VYQARDLRFPNVVKLCAVKEMVNHAPDPQMREIALQNFEREANILATLNHPAIPKVYD
                     YFSEDNRAYLVMELIDGRDLEAVISDGTGFLPQDQILAWAIQLCDVLAYLHSHSPPII
                     FRDMKPSNIMLDRHGQIRLIDFGIAKNFQPGQKGTMIGTEGYSPPEQYRGIADQRTDV
                     YGLGATLHHLLSRQNPQIEPPFSFHERPISAVNPNVYPEFAHVVMRALEYDPSRRYAS
                     PEEMKRALLVLKPASKLGTATIVSMAGRSDLTPLWTFTCEDEIRSGPALGGGAVFVGA
                     YDHNLYALEAQSGKFLWKYAAEAGIASTPCVAEDRVLFGSQDRVLYCVNMQTGRIQWT
                     CPSNGKIFSSPRVKFNHVFFGADDGMLYAVNFQTGRVAWKVQTEAAIRSSVCVGAEDI
                     FFGSNDGYIYALDIRGTLRWRYRTKRAIVSSPALHDDILYVGSDDANVYAIDARSGWV
                     VWRFRTGKAVWSSPVISESQLHVGSVDGNVYTLDIRTGREVWRYATEGQVTASPAVHD
                     GVVYIGSVDQSLYSLDARTGSLRWRFRTSGPITSAPAIGGDILYVTSTDRQVYALPI"
     misc_binding    16423..16696
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            16813..17523
                     /gene="alaXM"
                     /locus_tag="AC162G_10470"
     CDS             16813..17523
                     /gene="alaXM"
                     /locus_tag="AC162G_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaXM"
                     /protein_id="HIR5076285.1"
                     /translation="MTELLYHTDAYCREFDATVVAIEGSEVALDRTAFYPGGGGQPSD
                     AGVLSMGGRQWAVTKVRKQGDRVWHEIAEGEPPAAGSTVHGAIDWERRYRLMRTHTAM
                     HILCGVIWRDYGASVTGGNMEPLRGRMDFEFETMRQELVKDIEEKINVEVAAARPVRV
                     RILPRQEAFQIPDLIRTKINLLPEGIAEVRTVEIEGLDLQADGGTHVANTSEVGRIRV
                     VDYKSKGKINKRIEVAIE"
     gene            17531..20878
                     /locus_tag="AC162G_10475"
     CDS             17531..20878
                     /locus_tag="AC162G_10475"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR5076286.1"
                     /translation="MPFKPVSTTVDFPTLEREILQGWKDTDAFNKLRRLRASSGKRFS
                     FLDGPITANNPMGVHHAWGRTYKDVWQRYYAMKGCNQRWQNGFDCQGLWIEVNVERDL
                     GFTNKRDIETYGIAPFVVLCKQRVLTFAAVQTEQSIRLGYWVNWDDPDQLRGLRDKLG
                     ENPHQVIEYRGPDGGLEGTVEQVVGQLGLPHVGGSYFTFSDENNYQIWRFLKTCHDQG
                     WVYKGHDVMPWCWRCGTGISQHEIVTEGYIEKVDPGLTVRFPLAGHWGDGQTRESLLV
                     WTTTPWTLTSNVAAAVGPDLDYVKVRQTLEDGAEEYLYLSTGTLSMLKGAYEIVDELK
                     GREMEGWRYIGPFDDLPVARETGVPEAHRVILWDEVGVVEGTGIVHIAPGCGAEDFAL
                     GKQYNLPVIAPLDEDGVFVDGFGWLSGMHVKDVADPIADALKIKGRFYRQEDYTHRYP
                     QCWRCQTPLVFRLVDEWFIRMDGMRQLLIDVTEQIRWIPDFGKDRELDWLRNMHDWMI
                     SKKRYYGLALPIWECGVCGHFEVIGSKAELKERAVEGWDAFDGHTPHRPYIDAVKIRC
                     SKCGALVERIADVGNPWLDAGIVSISTMGYTYDRTYWETWFPADFITESFPGQFRNWF
                     YALLTMGAVLTGQPPFKTVLGFATLLGEDGRAMHKSWGNAIEFNEAADEIGADVMRWV
                     FVRQRYESDIWFGRKSAAEVRRQFFIPLWNVYSFFVTYANLDGWTPPMADGRRRMADS
                     DRTLLDRWILARLHELIGEVTAALDDYDAPRVARAVEPFVDDLSNWYVRRSRRRFWKS
                     ESDADKNAAYSTLYEVLLTLAKLLAPMLPFLAEAVYQNLARSFDAGAPESVHHCDWPA
                     ADAARIGAGLLADMAVARQVVTLGHATRANNNLKVRQPLGKAIVVVPEAQRSAVVRMS
                     DVIADELNVKTVELAANEAELVTYKLLPDNKKLGPKFGAKFPLVRKALAEADAPRVVE
                     AIRSGAPVTLGSGEDTVELAPDEILVTPQPRESLAVSSEAGAVVALDTALTPALVREG
                     LARDVVRRIQDLRKQAGFDISDRIVAAAQAEGELRAAIEAWQDYVKAETLSDELRFGA
                     PEPGMLTEEDSLDGMRLVIGVRKKPIT"
     gene            20971..21312
                     /locus_tag="AC162G_10480"
     CDS             20971..21312
                     /locus_tag="AC162G_10480"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR5076287.1"
                     /translation="MPVPYDQLKSALLSEQARLTEQMAALSEVAAGDHLGYGNHQADD
                     GSVAFEQAKDLAIRINAESLLKQVNGALRRLENGTYGLCVECNQPIDTARLKAIPYAD
                     LCISCQRKRER"
     gene            21313..21819
                     /gene="lspA"
                     /locus_tag="AC162G_10485"
     CDS             21313..21819
                     /gene="lspA"
                     /locus_tag="AC162G_10485"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR5076288.1"
                     /translation="MKRLPLVPLVALATLALDQFTKWLVVTSLALGESIYPLPALSRL
                     FSLTYVTNTGAAFGLLKEAGMVFVFVAIVVITIIVLYMRRVPADQQLVRVALGLQLGG
                     ALGNLIDRLRLGHVVDFIDLKFWPVFNVADSAIVIGVALLAIAFWRQERAAARSPEPS
                     SGSGQFPG"
     gene            21961..22470
                     /gene="lspA"
                     /locus_tag="AC162G_10490"
     CDS             21961..22470
                     /gene="lspA"
                     /locus_tag="AC162G_10490"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR5076289.1"
                     /translation="MKRLPTLPLLVAAIVALDQATKWWVVSYVPVGHYWFPIPALAHV
                     VAVEHVTNTGVVFGLFKDAGPLFALASTTIVIVMIGYLIRLPRDRRWARLALALLLAG
                     GIGNLIDRLRLGHVVDFIAIGSFSRFNVADSAVTIGVALMLVTALLDGRRPPAAKARA
                     ASDADSSQM"
     gene            22479..23402
                     /locus_tag="AC162G_10495"
     CDS             22479..23402
                     /locus_tag="AC162G_10495"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR5076290.1"
                     /translation="MPVHIITLHADTRERLDKFVAARVSDLSRTAVQRLIDDGLVTVN
                     GAVTGAAHKVGQGDEIVVRVPPPAPATIEAEDIPLSIVYEDADLIVIDKPAGLVVHPA
                     AGHDRGTLVNAILAHAPDLKGVGGEMRPGIVHRLDKDTSGLIVAAKHDAAHRELQRQF
                     KSRQVKKVYLALVEGRLAPRVGIVDAPIARDRVHRKRMAVSADGRPSRTRYKVIRYLR
                     LAEAAYSFVEAHPETGRTHQIRVHLAWLGHPLVGDTLYGRRKPALPIGRQFLHAARLT
                     LSLPSTGEERTFESPLPDDLERVLRSLGDYS"
     gene            23521..24333
                     /locus_tag="AC162G_10500"
     CDS             23521..24333
                     /locus_tag="AC162G_10500"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR5076291.1"
                     /translation="MREVTIACVQMQVELNRMEDNLVKMSEWIEKVAVAQKVDLIVFP
                     ELATTGYECGVRFTDFAQRVPGPSANLIAQRASEFGVYVAFGLPSKEKVESVLYNSAV
                     LVGPDGDLVGEYRKVHLKGEERLAFRNGFRFAVLETDFGNVGLMIGWDVAFPEAARTY
                     ALDGADLLIVCANWEKPNVEEWRTYVTARALENSIFVAASNRIGQDATLSFCGETMIV
                     GPRGQTFAQVDEEIEGYALARIDLDDVRRYREELQQIQNRQPSAYRAVVRQY"
BASE COUNT         4251 a         7929 c         7920 g         4307 t
ORIGIN      
        1 tgagcgtgag cgccgaggtg agccgcgcag tcacctccgt gctcgatccg gacgatgtga
       61 ttcgccgggt cgcacagttg atcacggagc ggttcggttt ctactacgcc gccgtgttcc
      121 tgctcgatga cttgggccgc ttcgccgtct tacgcgaggc gatcggcgcg gcgggcagca
      181 tcctcagggc cagccagcac cgcctggaga tcggcgggca gagcatggtc ggcgccgcga
      241 tcgctagtcg ccagccgcac gtggccagaa atgtgggcga cgagaccatg cgctttgcca
      301 atccgcttct gcccgacacg cagtcggagg tcgccctccc tctcctggtc ggcgatcgcg
      361 ttctgggcgc gctcgacgtg caaagccgcc aactggacgc gttcgacgag aactcgctgg
      421 ccatgctgca gagcctggcc gaccagatcg cgatcgcgtt gaacaacgcg cagcaatatc
      481 aggaagcgca gctcgacgcg cggcaggcga ccgcactgtt cgaggccagc cgcctggtcg
      541 gcttcatcag cgaagacctg tccgcggcgg ccgatcactt gctgcgcact gtcgccgagc
      601 aggcgggctt cgactcgtgg acggcggcgg cttacgaggc cgatagcgca acctacaccc
      661 tcctgtgtgc cttccaggac ccggctcgca ggctgtccgc ggagcatcgg catgacgcgg
      721 attttgacac ggatacacgg attggtggga tggatgcgcc caacgcgctg gccattcgca
      781 tgcgccaggc agtcgtcatc aacgatcccg aaatcgaccc ccttctgagc caggcgttgc
      841 ctgcagagcg cgaggcgtat ggccgatcgg tgagcgtgcc ggccgtcctg ggcgatcgcg
      901 tcgtcggcgc catcagcctt ggtcgcgcga tcgacaaaag gccgataagc gcgcgcgacg
      961 tgcaactggc gcaggctatc gccaaccagt tggcggtcac gattgagaac cgccgcctct
     1021 tcgagcagtc gcgcaaggca gtcgccgagt tgaacagcct gatgcaacgc tacacgcacg
     1081 agggctgggc gaggttcagc caggcggatc acgccaccgt cgagcatgag tttagacgcc
     1141 ccgcgcctgc cctgagccga agccatagcc gaagccgtga gcggagcgaa ggggcaggcg
     1201 aagggtcgcg cgtcgcgccc agcagcgagg atgcacggaa cgcgcgaggc gactccggtc
     1261 accgaccccc gcagacagcg ggcgagggca ggcagccggc gctggccgtg ccgatcgagc
     1321 tgcgcggcga agtcatcggc atgctggacg tgcaggccga gggagaccat cagtggtccc
     1381 aggacgagct ggtcatgctg aaatccgtgg ccgatcaggt ggcgcagtcg gtcgaggccg
     1441 cgcggctgct cgacgagact gaagccagct tgcaggaaac ggtgaagttg tacgaggcca
     1501 gccgcgcgat ggccgcggcc gccacgcccg ccgaagtcat gcataccgtc gtaagcgtac
     1561 tgcatggtta cctggaggta gaccaggtgt ccctcgtgct cttcgacgag gagcaggagt
     1621 acggacgagt cgcggcgcag gctgtgccgt cgcctggcgc ggagacgata cgggtgccca
     1681 tggccgaaaa ccccggcgcc ctcgccatgc tggccgacgg ccggtctatc gccatctctc
     1741 atttgaccgg ccatccggtg gcggctcccg tggcggatct cttgcgccag atgggcgccc
     1801 ttggcgagtc gtttctcgtg ctcccgctcg tcgtcgggca gcgattgatc ggcttcgtag
     1861 gggcgtcctc ttgcgggcgc cccacgccgc gcgagttccg ggacaacgag atcgccttct
     1921 gcgagacgct ctcccggcag gccgccgtgg ccctcgagaa ccggcgcctg ttcgaagaga
     1981 cgcgttaccg ggcggagatc gaatcgacgc tgaatcgaat cagccagacg atcaaggcgt
     2041 cgctcgaccc gcagacggtg ctcgacctgc aaaccacgat cgccgagatg ggtcgcgcca
     2101 tgcgcgccag tcgcagcttc tatctggcga acgtcgacga tcggcgtttc gtggtcgcct
     2161 acgaatggcg cgaagcggcc gacaatgccg cctcgctgag aggggtaacc ggcgtgtgga
     2221 tggatgcgcc aaacacgttt caagcattca aggcaggcga gccgctcctg gtcagcagcg
     2281 tcgaacgcca gggcgtgccg gggcttcgcc ccccgctgct cgccaaagac gccctgtcac
     2341 acatgcacgt gcccgtggtt tccggcagtc gcctgatcgg cgtcctgggc tttgaccagg
     2401 tcgagcgcga gcgccgctgg ctgccggaag aagtgagcat ggtgcggcgc gtagccgacc
     2461 agttggccgc agcccttgag aatgcccgcc tgtatcaaga cgtccgagcg cgcgtcaacg
     2521 agctgaccgc gctcacgcgc atcgggcgcc gcctggcggc ctcgcttcag ttggaggaag
     2581 tgctcaacac catcgtcgaa gaggcgatca acatcacacc cgccgaccgc ggctcgatcg
     2641 cgctgtacga tccgatcagg gatgcgctcg agctgcgtgt gctcatcggg ttccccgagg
     2701 cggcgctgag gacgatccgg gataacacgt acaccgtgct gcagcgtggg gatggcctgc
     2761 atggccgctt gctgacgacg gggcaggctg tcctcgtcaa cgatgtgctc agcgatccgg
     2821 cgtacattat cgcagacagc gacacgcgcg ccgagctgat cgtcccgatc cgccagggcg
     2881 aaacgctgct cggagcgctc aatttggaaa gcccgcacgc ttacgccttc ggcgagccgg
     2941 accagcgcct gatcgaggcg ctggccgacc aggcggcggt cgccatcgcc aacgcgcgcg
     3001 cctacgaggc cgagcgcgcc gccctcgatc gcatgcgcga ggtcgaccgg ctcaaaacgc
     3061 agttcctggc caacatgagc cacgagctgc gcacgccact caactcgatc atcggcttca
     3121 gccgtgtcat tctgcgcggc atcgatggcc cgctgacaga gatgcaacag gccgacctga
     3181 ccgcgatcca caactccggc cagcacctgc tcggcctgat caacgacgtt ctcgacctgt
     3241 cgaagatcga ggcgggcaag atggaactgg cgtcggagga catcgacctg gccgaggtgg
     3301 tcaggggcgt catgtctacg gctatcgccc tcatcaaaga caggaagatt gagctgcacc
     3361 aggagatccc gcccgatttg ccactggtga gagccgaccc gcgccgcatt cggcaggttg
     3421 tcctaaacct ggtgtcgaac gcggctaagt tcaccgagac gggccgcatc actacgcgcg
     3481 tcatcgtggg cacgaccgaa gtgtgggtct ccgtcgtcga caccggcatc ggcattcaac
     3541 cagacaagct ggagcacatc ttcgaggagt tcacccaggt ggatcccagc accacgcgca
     3601 aggcgggggg caccggcctg ggtctggcga tcagccgcaa gttcgtcgag atgcacggcg
     3661 gccgcatctg ggccgagtcg caaatgggcg ttggctccac cttcaccttc accctcccgc
     3721 tggcgcggcc agcgttgcac acggattcgg acacggatga cacggatacc accctgccgc
     3781 tccccgagcc cggccggccg ctcatcttgt gcatcgacga cgatccggag gttatcacac
     3841 tgtaccggcg tttcctggag aagcagggct accaggtgat cggcctgcgc gataccgagc
     3901 gggcagtgca ggaagcggcg cgcctgaagc cggcgaccat catcctcgac gtgctgatgc
     3961 ccgcggagcg tcgcgacggc tggtcgatcc tggccgagtt gaagagcaca cctgccacgc
     4021 gcgatatccc aatcgtcgtg tgcaccatcg tcgaagatca gaacaagggc tttgcgctgg
     4081 gcgcggccga ttacctggtc aagccggtta gcgaaagcga tctcatgcgc gcgctagatc
     4141 gggccagctt cagcaaagcg atccacaccg tgctggtcgt cgacgacgag ctggatgcta
     4201 tccgcctgct gcggcgtatg ctggaagggc ggcccggcgt gaacgtgatc gaggcgcagg
     4261 gcggcgccat gggcatcgcc gccgcgcaga cgtaccagcc tgacctcgtc ctcctcgacc
     4321 tgatgatgcc cgacatcgac ggcttcgcgg ttctcgagac catccggggc aaccgtcaca
     4381 cccacgacat cccggtcatc gtcgtgacgg ctaaagatgt gaccgaggaa gatcgagcgc
     4441 gcctccacgg caaggtggtc gcgatgttga ataagggcgc cctcgattcg gagcggctaa
     4501 tgaatgagat cgctgctgcg cttgcccgcc tgaaccgccg gcagcccgcc ccagccctcc
     4561 ctcgtgaaac gggagagagg ccgggggcga ggggcgcgca gcgcgaccta cctatagaaa
     4621 atcatgtgaa gagtgagcga ccattatgat gctcgaccgc cgtttgaggt tcgatccggt
     4681 actcgaatgc ctgcgctgcg gcacgcgcct gcctgtacct gtggcgcagg cacaggtggg
     4741 cgaagcctac cagccgctgc ctgccgcgtg ccccaactgc gatttcacga tgctggacgt
     4801 tcggtacgac tacgatgctc ttcgcgatag cctgcccgga ctactgcgcg accggatgtt
     4861 caacatgtgg cgttatctgg agctgctgcc cgtgcgagac ccggccagca tcgtcacgat
     4921 gggcgagggc ggctcgcccc tgatccggct cgccaacctg ggcgtcatgc tcgggcgcag
     4981 ccgcatcttc atcaaagacg agcgccaggg gccgaccggc tctttcaaag atcggcaggc
     5041 gtcgctggcg atctcggtca tgaaagagag cggcatcctc gaatgcgtcg tcgcctccac
     5101 cggcaacgtc gccatctcgt actcggccta ctgcgcgcgc gctggcatca agttgtgggc
     5161 gtttctcacg agcatggtgc cggccgaaaa gatgcgcgag gtggctctct acggctcgga
     5221 agtggtgaaa gtcaccggca cctacgacca agccaaggag gtcgccgcga cgttcgccca
     5281 gcaccgcggc ctgtatctcg accgcggcat ccgatcgatc gcggcgcgcg agagcatgaa
     5341 gacgctcgcc tacgaagtca gcgagcagct cgcgctggtc gatcacagcg tgcacaacgg
     5401 gcggcggccg ctcttccgcg cgccggattg gtacatccag tccgtcagcg gcggcctggg
     5461 gccgctcggc gcgtacaagg gatttggcga gctgaagcgg atgggcttca ccgatcggat
     5521 tccgaagttt gccctgatcc aggtggaagg ctgtgcgccg atgtcgcgcg cgttcaaagc
     5581 cggcaaagag gttgccgacg tcgtcagcga tccgcagacg cacattgcca ccctatccac
     5641 cggcaacccc ggcccggcct acgctttgtt gcgctccatg atcctggagc acgacggaac
     5701 gatggatatg gtgagcgacg aagacgcctt ccgcgccatg cacatcacgg ccaagatgga
     5761 aggcatctct gtcgagccgg ccgcagccgt cgcgtttgcg gggctgttca agctgatcga
     5821 ggcgggattg atccgacccg acgaggacgt cgtcgtcaac tgcaccggcc acacgctgcc
     5881 tgtcacgcag gaattgcttg gcgacgagtg ggctttgtcg ttcgagatgc cgacggcccc
     5941 gggccctgcc gcgcccctgc cccaggaggg tttgctctcc gcgctcgagc gcctggacaa
     6001 gcgcgtgaac cgtatcttga tcgtcgacga caacgccgat gcgcggcggc tgttgcgccg
     6061 catcatgcag gcgcgtggcc gttatacgct gttcgaggcg gccgacggcc gccaggcgct
     6121 ggcgatggcc gccggcgagc ggcccgacct gatcctgctc gacctgatga tgccggagat
     6181 ggacggcttc caggtcctcg aagcgctgaa gcagtccgaa tccctacgcc agattccggt
     6241 catcgtggtc acggctaagg agctgacgcc gctcgagcgc cagcggctgg ccgggcaggc
     6301 ggattccctg ctgcagaaag gctcgttcac cgatctggac agtctggacg atctggtcga
     6361 tgccttgata tgacacggat tcggacacgg atacatggag gatttgaacg gacgggatgg
     6421 acgcgacgga tcatcagaag cgggttctat atatcgagga cgatccaggc agccggctgc
     6481 tggtcaagcg catcctggag agcgaaggat acgtcgtcga cgaggcggcg gatgggctct
     6541 ccggcatctc gtccgcgcag aagaacctgc cggatctaat cctggtggac gtcaacctgg
     6601 gcgaggtctc cggacacgac gttgccacca agctcaaggg gatgcccgag acggcgcgca
     6661 tccccctcgt cgccctgacg gcggccacca tcagcggcga tcgcgagcgc gccctcgccg
     6721 ccggatgtga cggctacatc cccaagccga tcgacgtcga ccggctgccc aatctcgtgc
     6781 ggcgcttcct gggcggcgcg cgcgagcgcg tcgccgacag cgtgcgcgcc gagaggctgg
     6841 aggagtacag ccgcacgctc gttgatcggc tgcaggcgac ggtcgccgac ctgcaaaagg
     6901 cgaacgccga gctgcggcgg ctcgacaaga tgaagagcga cttcgtggtg ctggcgtcgc
     6961 acgaactgcg caccccgctc acactcgtct acggctacgt caatttgctc aagctggaga
     7021 tcggccgctt gcccgataac gcccacttgc tcgacgtcac gcagcgcctg agcgacgcga
     7081 ccacgcgctt gaccgagctg tacgacgcga tcgtgaatgt ctcgctcatc gacacgcagc
     7141 gcctgaacgt gacgctcgta ccgatcgatc ttttcggcct gattgcatcg gtggtcagcg
     7201 agctaagccc ggtggcgcgc cagcgcagcc tgcgcctgaa cgtcgccgat tttcgcggcc
     7261 tgccgaagat cctggccgac gccgactatt tgcggcgcgc gatctcaaac gtcgccggca
     7321 acgccgtcaa gtacacaccc gacggcggaa cgatcggact ctcgtatcag cttcagccag
     7381 acgcgatcga catcgtcgtg accgatagcg gcatcggcat cgacccggcc gaacacgagc
     7441 gcatttttca gaagttcgcc gtgacggaag atatccggta tcactccacc agccgcacgg
     7501 cgtttcaagg cggggggctg ggcctgggcc tggccgtcgt gcgcgggatt gtcgaagcgc
     7561 acggcgggcg cgtgtgggtc gagagcgaag ggcacgatcc ggcgcggctg ccggggacaa
     7621 cattccacat tctcctgccg gcgtcgggtc gtcggcgacc ggagtcgcct ccgacaaagt
     7681 cgcctccgac agagtcgcct ccgacagagt cgccgcctac agccgatagc gtgcgcggag
     7741 cggcggtatg acgtcccgcc gacgcgcgca gcagcgtggc atcgccgccg ccctgctatc
     7801 ggccgtgttt ctggggacgg cgccgatctt cggcaagcag gcgtatgccg ccgagatgac
     7861 gccgattgcg ctcgccgcct ggcgcatctt gatcgccgcc gccggtctgt ggctcgtcta
     7921 cgcgctgatc ggccgccgct atttgtacat ttatcctgcc ggcctgatcg gctgcgtggc
     7981 cgtcggcgcg gtgaacggcg tcggctcact gatgtactat tcgggattgg aacgcgtgga
     8041 tgccggcgtg ggccagatga tctacagtct gtatccgctt ttcctggtca tgtttctgcg
     8101 cctcgacggg catcgtttct ccggcctgac gcttttccgc atggtgctag cggtcgtcgc
     8161 catcgccctg atcacgcagg cgggcggcgg cccggtcgac gtcgtcgggt tgggactgat
     8221 gctgggggcc gggttcggct acgcgctgca cctggcgatt ggccagcgcg tgagctacga
     8281 gatgccggcg cagacgttga cactgtacgc ggtgacttcg atggcggtgg tggtgtgcgc
     8341 ggccagcctg atcgccgggt ggtcaccggt gcctgctggg gcgtggccgc cgacgctcgg
     8401 cctggcgtgg gtgacggtcg cctcgcggct gggcatgttc ctcggcgtga agcggctggg
     8461 cagcctgcag acagcgctca tcggcatcac cgagatcctc gtcaccctga tcctggcgct
     8521 tacgcttctt ggcgagacgc tgagcagccg gcagtggatc ggtgcggggc tgctcatcgc
     8581 cagcttgctc ctgatcggcc gcgagccgac gttgggcggg gcgctcccgc gcaagatcca
     8641 atctgccgcc tcgcgctcct cgtcgatagc ggccgaatcg cagtaaaacc aataccgttc
     8701 aaattggaag cgatcagcac gactttgtcg ccgtcttcga ggccgacgag tgggagcgag
     8761 cggggcaggc gactcctggc gccagtcgtc gtgagtgaca ccacaggcaa gccggtgagg
     8821 ataccggttg cagagtatcg accgttcgag agtcgcagca gcgggcggtc gacgtgatgc
     8881 aggatgtgcg aggagagcca cgatgcggcg cgcgtcgccg cgatttgctt gatcgcgagg
     8941 cgccagccgc gctcccgggc ctgactgttc gccatgcgct cacgcgttca ggcgatagaa
     9001 tcggaagaag aagttgtcga cgggcaggat ttcgacgctg ccaaaacccg cttcggctgc
     9061 gtagcgtctc agcgtgtcgg cgcgcatgac ggtgcctgtt cccgccgagg gccggtcggc
     9121 catgcctacg ggtaaacagt gcaggacgct ccagccgtac atcatccact cgacgtcatt
     9181 ccctttcgcg gtgaaggact cgcccacacg ttcgtcgacg atcaacaccg tgccgcgctc
     9241 cccgctcaag cggcgcatgg ttcgcagtgc gccgaccggg tccgacatat cgtgaacaca
     9301 ctcgacggcg gtcacgagat cgtacgctcc ctcgagcgcc gggtcgcccg cgtcacgcac
     9361 ctggaagtga acccggtcgg tcaagcccca tgcccgggca accaggcgcg cgcgctcaat
     9421 tgaaggtgcg tccaggtcga atccgtccac gaggactctg ggataaccct gtgcgatgcc
     9481 gatactcgac cagccgtagc cgcacccgat gtcggcgacg cgcgccggcg gatccgcctg
     9541 caagcgcgcg tgcacgtcag gcatagcggg gagccattcc tgcgccaact ggcgcgtgaa
     9601 cgcggggcaa ttgatggcag cctgtccttc gcggagatta gcgccgtagg cgctgaaggg
     9661 cacgcctccc ccgttgcgat agacgtccag cacggcctcg atagggtgga cggcgccgac
     9721 gatgagctga accaggggcg caaggtagtt caagctgtcg cagtcgatca gcacttcgac
     9781 gtgtccgggg ggcaggcgga atctgcgcgc ggcggcctcg cggttctcgt cctcgacctc
     9841 cagaatgcct gccaccgtct gctgttcgag ccattcgcgc acgtagcgtt cctgcatgct
     9901 cgtgcgagtg gcgagttcgg ctgccgtcga tggccctccc tgtgcaaggg cacggtacag
     9961 gccgaggcga tcgccgatgt agatggtgaa gatgttgaac gtgcccgacg caaaatgaag
    10021 aaatcgctcg acgaacgcgt cgcgctgccg ggcaacggca tcggtagata cttgaggtga
    10081 atcgctcatg actcgtcctt tccctgccac gcctggatct gtggaatgtg ctgcgggtaa
    10141 tgctcgtagg aattttctgc gatgagtttc gcgggctgcc agtcgggcgg catccctgct
    10201 caccgctcac ctgacttgaa gtccacgaac cgatacccca cgccgcgctc ggtaaagata
    10261 tacctgggat ttgcaggatc cggctccagc ttctggcgca ggtaagtgat gtacagccgg
    10321 agatagtgcg tctcgtcgcg gtactcgtag ccccatactt tcgacaggag cgtgtcgtgc
    10381 ggcacaatgt gcccggcgtt ctgcaccagg tgatacagaa gccggtattc cgttgggcgc
    10441 agcttcactt ctttgccgcc tgcaatcacc cgccggttcg cgaaatcgat ctggaggtag
    10501 tcgtccaccc ggatcgttga cttctcgacc aggccgggca gctcggcccg gcgcagtgcc
    10561 gccttaatgc ggctcgtcag ctcgcggggg ctgaacggct tcgtcacgta atcatccgca
    10621 cccagctcca gtcccttcac cttgtcgtcc tcgtcgtcct tcacggtgag catgatcacg
    10681 ggcacggtcg acgtctcgcg taccaggcgc agcgtctcga agccatccag gtcgggcatc
    10741 atcacgtcga gcacgactag gtcgggcagc tcgtcgcgca cctggttcag cgcctcgagg
    10801 ccgttcgtcg cctgcacgac gcggtatccc tccagctcca ggttcatgcg gatgaactgg
    10861 atcatgcgcg gctcgtcgtc gacgaccagg atggtgtgct tggcttctgg gggcatgacg
    10921 gaggatggta gacctgttag gtcttcaaga cctaacaggt ctggacaggc tactcgcgca
    10981 cgaagccgat gacctcgcct tcctcttcgg tggtcatcac ttcgatgaat gcgatgcggg
    11041 tccaggggaa aatgaccgac gcggcgctcg gatcgatgaa caggacgtcc ttgccgtcgc
    11101 gccggcgcac gttcgtcacc agcacgaagt tatcggtcgg gtggggaagc tcgtccacct
    11161 cgcccacgac cggatcctcg ttgtgcaaat gaatcagaat cgtgtacggc atggatcgtc
    11221 tgcctttctg cgggagtagg tcgtagcgct attgcacgct gatgcgcagc cgcacgcgcc
    11281 ccaattgcac ctcgtcgccg tcgcgcagcg ggtgcggcag atagggcgtc aaccgctggc
    11341 cgttcaggaa cgtgccgttg gcgctgccca ggtcttcgat gaagaactgc gacttctgct
    11401 ggtggacgcg cgcgtgccgg cgcgacacgc cgccttccaa ccccccgtcc ggcgtgagat
    11461 cgacgtcggg gaacacgccg cgcgtcgcgt ccagccggcc gagcacggct tctccgccca
    11521 gcggcagcgc cacgcgccgg ccagtcgccg acatcaccac ggtcagcgtc gtctgctccg
    11581 attcggccgt ctgtgctcca ctgctgctcg cccacggatc ggcgcgcgcc gtcggcagtt
    11641 ccgattcggg cagcggctcg gtgcgcagcg ggccgcccgt cagcaggtat acgccgcact
    11701 cgctgcaaaa cagcgtgccg ggccggtgtt gtcgtccgca agatgggcac tcaatcatga
    11761 tttttgatcc ttcatagccg ccaaagataa gccgcgcgtg ccgtacttga tcgtcttctg
    11821 accgcccggc gacaggtgac cggagcgcgc cagccgcccc gcctcaagca gcgttgcgcg
    11881 cgccagttga tgctcgccca catccaggag ccgcgtcgcc atcgtctcca gccgttgcgt
    11941 tgcggcagcc acgtcgccct tctccagcga cgtccacgcg ctttccagca tctggaagat
    12001 cgtcaccttc gccagcgcgt tcagcaggtg cggcggcacg gcctgccccg gcggcggctc
    12061 cgtcgtaaat gtggcggaga tgttgcgctg cacgcgctcg tccgtgcgct ccagcgccgg
    12121 cacgttgccg gtcaactcga gctgcgccag ccggcgcacg ccaggttctc gctgcccgac
    12181 gaccagctcg aacagccccg cgatcgatgc gtcggcctgc agcgtcccta gcagcaatgc
    12241 gtcgccgtcc atgtccatgc gctggatgta cggcgtcagg cggaacgccc cgcgtatccg
    12301 cacgttatcg ctcaacctcg gcgtcagccg caggtcgtct ccgtacacgg tggccagcga
    12361 cttcagccgc tcgttcaaga tggctggcac gtctgcggcg gacgcgatgt agtgcgacgc
    12421 gccgcccgag cgcgacgcga tcgcgtcgag aagctggtcg ttccagtctt ccccgatgcc
    12481 catggcgctg atgccgacgc tgcgccgctt cgcctccagc gccagctcca ggcacttctc
    12541 ctcgtcgcca tacgtctgcc cgtcggtcag caggatcagg tgattgaccg actgcaggcg
    12601 gcggtggcgc tccagctcgc ccatgccgag cttcaggccc tgcaggattt ccgtgccgcc
    12661 tgccgcctgg atgccggtga cacgggactt ggcgcgcatc gtgtcgccga ccgtctggcc
    12721 gggccacacc acatccgccc ggtcgctgaa cgcgacgacg gccagcgcgt cgccttcctg
    12781 gagcgaatcg atcaactggt gtgcagcggc cttcacttgg tcgaggcgca ccccctgcat
    12841 cgacgtgctg cgatcgatca ccaggcacag gttgagaggc agccgctgca ccctcgacat
    12901 cgcccgcgtc gccttcacct cgaacaacgc gtacagaatc tgctcttcgg gtatgacctg
    12961 caagatcgta cggctgagct gcacgtcgag cgtgaacagc gcgtcgaagt ttcggccggc
    13021 cagccccaac tgccggtcgt acgcggtccg gcgctgatcg tcgctcaaca cctcgtaggc
    13081 ttcctgcacc tgccggaaca gcatcgacgt gcccgccggc gtctcccgag catccgggtg
    13141 aaactgcttg gcgagtacgc gatacgcgcg cttgatgtcg tccaggctcg cgttctgcgg
    13201 cacaccgagt atttcgtaga agtcaggttc ttcgatcata gcaggacttc agaccagttc
    13261 ggaatagcga cttgctggcg attgcccact ccgctttcct tcggctgcct cttcgctccg
    13321 ctcagggctt cggctagggc ttccgtttcc tgctcccctt ccccttccct ccgctcaggg
    13381 tcagggtccg ggacttcgca ggacaggcgc tgtggggtgc ttcgcaaaaa tcgtgccgac
    13441 aactgcgcag agtcgcccgg cggcgactgg caataacccg cgctggcggg ctttgtgcct
    13501 catgtgctcg ccggccgcac aggctcaggc gtcgacttcg acgacaatcg ccgtgatgtt
    13561 gtccctgccg ccggcctcgt tcgccgcatc cacgagctga ttgcaggcgg cttgcgccgt
    13621 cgctgcgtgg ctcacgatcc gggcgatctc ctcgtcgctc agcatccccc acaacccgtc
    13681 gctgcacagc aacaaacagc cgccgcgtgg gatcgtctcc acgtgcgtgt cgacttcgag
    13741 caggccctcc tggccgaccg cccggtagag cacgttcttt tgcggatgca ctgctgcttc
    13801 ttcggcggtt aactggccca gctctaccag cttgtcgaca tacgagtgat ctctcgtgat
    13861 ctggtgcagc acgccgtcgt gatagacgta cgcgcgactg tcacccacgt gcgcaatcac
    13921 cgcgcgctcg ttcacgacga gcaggcacgt gagcgtcgtg ccgccccccg gcacgttgtt
    13981 atacaccatc ttgctcgctt cgtccactgc ctgcgtgagc gcctccgcca gcggcgtctg
    14041 cgtcgtgccg ggcctgccgt gaatcagata cggcgtgtac acatgctcga gcacctcgcg
    14101 cgccaccgtg cgcaccgcga gcgagctggc cacttcgccg gcctgatgtc cccccatgcc
    14161 gtcggccagg atgaacagcc cgaacgcgtc gagcgccgcg tctccctgca gctccgacgt
    14221 gaaggtgaaa agcacgtctt cgttgtgtgt gcgcacgcgc ccgacgtgag tggccgatcc
    14281 ggcccgcagg tgcggcgccc ttgcggcggc cggggccgcc ccgcgtggcg atctgtggtc
    14341 catgcgattc tcttctcggg ttcggagcaa ccgtcgcacg aagcgcatag cgtgtctccg
    14401 gtgccctcag ataggcagtg cgtacacctg tcgatcggtc gacgtcacat aaagaatgtc
    14461 cccgccgatc gccggcgcag acgtgatcgg gccgctggtg cggaaacgcc agcgcaggct
    14521 gccggtgcgg gcgtccaggc tgtacaagga ctgatcgacc gagccgatgt acaccacgcc
    14581 gtcgtgcacg gcgggcgacg ccgtgacctg gccttccgtc gcatagcgcc acacctcacg
    14641 gcctgtccgg atgtcgagcg tgtacacgtt cccgtcaaca gagccgacgt gcaattggct
    14701 ctcgctgatc accggcgagg accacaccgc cttgcccgtg cggaagcgcc acaccaccca
    14761 gcccgagcgc gcatcgatcg cgtacacatt cgcgtcgtcc gagcccacgt acaagatgtc
    14821 gtcgtgcagc gcgggcgacg aaacgatggc gcgcttcgtg cgatagcgcc acctcagcgt
    14881 gccgcgaatg tccaacgcgt agatgtaccc atcattcgag ccgaagaaga tatcctcggc
    14941 tccaacgcac accgacgagc ggatcgcagc ctcggtttgc accttccagg cgacgcgtcc
    15001 ggtctggaag ttgaccgcgt acagcatgcc gtcgtcggcg ccaaagaaga cgtggttgaa
    15061 cttcacgcgc ggcgacgaaa atatcttgcc gttgctcggg cacgtccact ggatgcggcc
    15121 cgtctgcatg ttgacgcagt ataacacacg atcctgcgac ccgaacagca cgcgatcctc
    15181 ggcgacacac ggcgtcgaag cgatgcccgc ttcggcggca tacttccaca ggaacttccc
    15241 cgactgggct tcgagcgcgt acagattgtg gtcatacgcg cccacgaata cggcgcctcc
    15301 acccagcgcc gggcctgatc ggatctcgtc ttcgcaggtg aacgtccaca gcggcgtgag
    15361 gtcgctgcgg cccgccatgc tgacgatcgt cgccgtgccg agcttgctgg ccggtttcag
    15421 cacgagcagt gcgcgcttca tttcctcggg cgacgcatag cgccggcttg gatcgtattc
    15481 cagcgcgcgc atcaccacgt gggcgaactc ggggtacacg ttcgggttga cggccgagat
    15541 cggccgctcg tggaacgaga acggcggctc gatctgtggg ttctgccggc tgagcaggtg
    15601 atgcagcgtc gcacccagcc cgtacacgtc ggtgcgctga tcggcgatgc cccgatactg
    15661 ctccggcggg ctgtagccct ccgtgccgat catcgtgccc ttctggccgg gctggaaatt
    15721 cttggcgatg ccgaaatcga tcaggcggat ttgcccgtgc cggtccagca tgatgttgga
    15781 tggcttcatg tcgcggaaga tgatcggcgg cgaatgcgaa tgtaggtagg ccaagacgtc
    15841 gcacaactgg atcgcccagg cgaggatctg gtcttgcggc aggaaccccg tcccgtcgct
    15901 gatcaccgct tccagatcgc gcccgtcgat gagttccatc accaagtagg cgcggttgtc
    15961 ttcggaaaag tagtcgtaga ccttggggat ggccggatgg ttgagtgtgg cgaggatgtt
    16021 ggcctcgcgc tcgaagttct gcagcgcgat ctcccgcatc tgcggatcgg gcgcgtgatt
    16081 gaccatctcc ttgacggcgc acagcttcac gacgttcgga aagcgcaggt cgcgcgcctg
    16141 gtacacggca ctgaatccgc ccatacccag tacgctgcga atctcgtacc ggctctggag
    16201 cacgcgcccc ggcgcgagcg tgccgccggc ggtgggttcc tgttcggtgc cgatgtggcg
    16261 cgtgtcgagc gagctcatat cacatgcgac taacaaattt cggcgtacgg atagaccgca
    16321 cccctgtgta ttatagcgcg gttcaaaagc gtctgcaata aagataccat gaccgttcta
    16381 ttgccgactt gacgctgccc tgcgtctatg gcataatcct ttctatcagc gcagatggaa
    16441 acgagtacgt atccgggcct tcccagcgag ttcgggggtg gtgcgaaccg aacgaggcga
    16501 agatacggaa aatccttccg gagctgctga ccgaaaggtt ggcaagtgca gccgagtagg
    16561 ctcagccggg ctcgcggccc gttatccgcc gcgcggctct acgccgtctc gcagcgcgtg
    16621 cagccggtcg gagtggccca ttcgatttgg gcaatagagg gtggtaccgc gggagcctga
    16681 gcctctcgtc cctgttgtgg acggaggctt cttgtttccg tcgaaggcgc gaagttgcta
    16741 agaccatcag gggcgatgct gttcgcacgc caaaatatgt ggccgccgcc ggctctgggg
    16801 atggagggtg acatgaccga gttgctgtat cacacagacg catactgtcg ggaattcgat
    16861 gcgaccgtag tcgcgataga gggcagcgag gtcgcgcttg atcgcacggc tttctacccg
    16921 ggcggcggcg gccagccgag cgatgcaggc gtgctgtcga tgggcggccg gcagtgggcg
    16981 gtgacgaagg ttcgcaagca aggcgacagg gtgtggcacg agatcgccga gggcgagccg
    17041 cctgccgccg gctcgaccgt tcacggggcg atcgattggg agcgccgcta tcggctcatg
    17101 cgcacacata cggccatgca catcctgtgc ggcgtgatct ggcgcgacta cggcgcaagc
    17161 gtcaccggcg gcaacatgga gccgctcagg gggcgcatgg acttcgagtt cgagacgatg
    17221 cgccaggaac tggtgaagga catcgaggag aagatcaacg tcgaggttgc cgccgcgcgt
    17281 cccgtgcgcg tgaggatcct tcctcgccag gaggcttttc agattcccga tctcatccgc
    17341 accaaaatca acctgctgcc ggaaggcatt gccgaggtgc gcacggtcga gatcgaaggg
    17401 cttgatctgc aggccgacgg cggcacgcac gtggcgaata cctccgaggt cgggcgcatc
    17461 cgcgtcgtcg attacaagag caagggcaag atcaacaagc gcatcgaagt ggcgatcgag
    17521 tgaggatacc atgccattta agcctgtgtc cacaacggtc gattttccca ctctggagcg
    17581 cgagatcctc caggggtgga aagacaccga cgccttcaac aagctgcgcc ggctgcgcgc
    17641 ttcgtccggc aagcgcttct ccttcctcga tggaccgatc accgctaaca acccgatggg
    17701 cgtgcatcac gcctggggcc gcacttacaa ggacgtgtgg cagcgctact acgcgatgaa
    17761 gggctgcaac cagcgctggc agaacggctt cgactgccag gggctgtgga tcgaggtgaa
    17821 cgtcgagcgc gatctgggct tcacgaacaa gcgcgacatc gagacgtacg gcatcgcgcc
    17881 gttcgtcgtc ctgtgcaagc agcgcgtgct gactttcgcc gctgtgcaga ccgagcagtc
    17941 gatccgcctg ggctactggg tgaactggga cgatcccgac cagcttcggg ggctgcgtga
    18001 caaactcggc gagaacccgc accaggtgat cgagtaccga ggccccgacg gcggactcga
    18061 aggcacggtc gagcaggtcg tcggccaact gggcctgccg catgtcggcg gctcgtactt
    18121 cacgttcagc gacgagaaca actaccagat ctggcgcttt ctcaagacct gccacgatca
    18181 gggctgggtg tacaaggggc acgacgtgat gccgtggtgc tggcgctgcg gcaccggcat
    18241 cagccagcac gagatcgtca ccgaaggata catcgagaag gtcgatcccg gcctcaccgt
    18301 gcgcttcccg ctggccggcc attggggcga cggccagacg cgcgagtcgc tgctcgtgtg
    18361 gaccacgacg ccgtggacgc tcacctcgaa cgtcgccgcc gccgtcggcc cggacctcga
    18421 ctacgtgaaa gtcaggcaga ctctggagga cggcgcggaa gagtacctgt atctctccac
    18481 gggcacgctg agcatgctga agggcgcgta tgaaatcgta gatgagttga agggcaggga
    18541 gatggagggc tggcgctaca tcggcccgtt cgacgacttg cccgtcgcca gggaaacggg
    18601 cgtaccagag gcgcatcgtg tcatcctgtg ggacgaggtc ggcgtcgtag aaggcaccgg
    18661 catcgtccac atcgcgccgg gctgtggcgc ggaagacttc gcgctcggca agcagtacaa
    18721 ccttccggtc atcgcgccac tcgacgagga cggtgtgttc gtcgacggct tcggctggct
    18781 gagcggcatg cacgtcaagg acgtggccga tccgatcgcc gacgcgctca agatcaaggg
    18841 ccgcttctac cggcaggagg actacacgca tcgctacccg cagtgctggc gctgccagac
    18901 cccactcgtg ttccgcctgg tcgacgagtg gttcatccgc atggacggga tgcgccagtt
    18961 gttaatcgac gtgaccgagc agattcgctg gattcctgac ttcggcaaag accgcgagct
    19021 cgactggctg cgcaacatgc acgactggat gattagcaag aagcgttact acggcctggc
    19081 gctacccatc tgggagtgcg gcgtctgcgg tcacttcgag gtgatcggct cgaaggccga
    19141 gctgaaggag cgcgctgttg aaggctggga cgcattcgat ggccacacac cacaccgccc
    19201 gtacatcgac gctgtcaaaa tcaggtgctc gaagtgcggc gcgctcgtcg agcgcatcgc
    19261 ggacgtcggc aacccgtggc tcgacgccgg gatcgtgtcg atctcgacga tgggctacac
    19321 gtacgatcgc acctattggg agacgtggtt ccccgccgac ttcatcaccg agtcgttccc
    19381 cggccagttc cgcaactggt tctacgcgct gctgacgatg ggcgcggtgc tgaccgggca
    19441 accgccgttc aagaccgtgc tcggcttcgc tacgctgctc ggcgaggacg gccgcgccat
    19501 gcacaaaagc tggggtaacg cgatcgaatt caacgaggcc gccgacgaga tcggcgcgga
    19561 cgtgatgcgc tgggtgtttg tgcgccagcg ctacgagagc gacatctggt tcgggcgcaa
    19621 atccgccgcc gaggtgcgcc gccagttctt catcccgctg tggaacgtgt actcgttctt
    19681 cgtaacgtac gcgaatctcg acggctggac gcccccgatg gcggatggca gacggagaat
    19741 ggcagatagt gatcggacgc tgcttgaccg atggatattg gcgcggctgc acgaattgat
    19801 cggcgaggtg acggcggcgc tcgacgatta cgacgcgccg cgcgtggccc gcgccgtcga
    19861 gcctttcgtc gacgacctca gcaactggta cgtgcgccgc tcccgccgcc gcttctggaa
    19921 gagcgagtcg gatgctgaca agaacgccgc ctacagcacg ctgtacgagg tgctgctgac
    19981 gcttgccaag ctgctcgccc cgatgctgcc ttttctggcc gaggccgtgt accagaacct
    20041 ggcgcgcagc ttcgacgcgg gcgcgccgga gtcggtgcac cattgcgact ggcctgccgc
    20101 agacgctgca cggatcggcg ccggtctgct ggccgatatg gccgtcgccc ggcaggtggt
    20161 gacgctgggc catgcgacgc gcgctaataa taacttgaag gtgcgccagc cgcttggcaa
    20221 ggcgatcgtc gtcgtgcccg aggcgcagcg atcggcggtc gtgcggatga gcgacgtgat
    20281 cgccgacgag ttgaacgtga agactgtcga gctggcggcg aacgaggccg agctggtcac
    20341 ttacaagctg ctgcccgaca acaagaagct ggggccgaag ttcggcgcga aattcccgct
    20401 ggtgcgcaag gcgctggccg aggccgacgc gccccgcgtc gtcgaggcca ttcgctcggg
    20461 agcgcccgtc acgctcggca gcggcgagga cacggtcgag ctggcgccgg acgaaatcct
    20521 cgtcaccccg cagccacgag agagtctcgc cgtctcctcg gaggcaggtg ctgtcgtcgc
    20581 gctcgacacg gcgctgacgc cagcgctcgt gcgtgagggc cttgcccgcg acgtcgtgcg
    20641 ccgcatccag gacctgcgca agcaggctgg cttcgacatc tccgatcgca tcgtggccgc
    20701 cgctcaggcc gagggcgagc tgcgcgccgc gatcgaggcg tggcaggact acgtcaaggc
    20761 cgagacgctc agcgacgagc tgcgcttcgg cgcgcccgag ccgggaatgc tcaccgagga
    20821 agacagcctg gatgggatgc ggctggtgat cggggtgagg aaaaaaccga ttacgtgatc
    20881 acgtgatcac gtaatcaacc atcagccacg tattccctac cttgtcggaa ctggactcgt
    20941 cgggtagaat acgcgaaagg ggagggcgac atgccggttc cgtatgacca actcaaaagc
    21001 gcattgttat ccgagcaggc gcgcctgacc gagcaaatgg ctgcgctgag tgaagtggcc
    21061 gcaggtgacc atctcggtta cggcaaccac caggccgacg acggctcggt tgcgtttgag
    21121 caggccaaag acctggccat ccggatcaac gccgagtcgc ttctcaagca agtgaacggc
    21181 gcactgcggc gcctggagaa cggcacgtac ggcctgtgtg tcgaatgtaa ccagcctatc
    21241 gacaccgccc gcctgaaagc catcccctat gctgacctgt gcatatcctg tcagcgcaag
    21301 cgagagcgat aattgaagcg gcttccactc gtccccctcg tggcactggc tacgctcgcg
    21361 ctcgaccagt ttaccaagtg gttggtcgta accagccttg cgctgggcga gtcgatttac
    21421 cctcttccgg cgctgtcgcg cctcttcagc ctcacctacg tcacgaacac cggcgccgca
    21481 ttcgggttgt tgaaagaggc cggtatggtc ttcgtcttcg tcgccatcgt cgtcattacg
    21541 atcatcgtgc tgtacatgcg cagggtgccc gccgatcagc aactggtgcg cgtcgccctt
    21601 ggcctgcaac taggcggcgc tctgggcaac ctgatcgacc ggctgcggct gggtcacgtc
    21661 gtcgatttta tcgacctcaa gttctggccg gtcttcaacg tcgccgatag tgccatcgtg
    21721 atcggcgtcg ccctgcttgc catcgcgttt tggcgccagg agcgcgccgc cgccagatcg
    21781 cccgagccct cttctggcag tggacaattt cccggctgaa cgggccgcgc cctctccgcc
    21841 gcttagcctg gaattcgcac ccgctcccgc cggattgcca catccggcgt ggattgacca
    21901 atccacgccg agcaggcgaa atatgagctt aagccgggcg gcccctttag gaatatagcc
    21961 ttgaagcgac ttccgacgct gcccttgctc gtcgccgcca tcgtcgcgct cgatcaggcc
    22021 acgaaatggt gggtggtgag ctatgtgcca gtgggacact actggttccc gattccggcg
    22081 ctggcccacg tcgtcgcagt cgagcatgtc acgaacaccg gcgtcgtgtt cgggttgttc
    22141 aaagacgcag gccccctgtt cgcgctggcc tcgacgacga tcgtcatcgt catgatcggg
    22201 tatctgatca ggctgcccag agatcggcgt tgggcgcgcc tggcgttggc gttactcctg
    22261 gcgggcggca tcggcaacct gatcgaccgg cttcgcctcg gccacgtcgt cgacttcatc
    22321 gccatcggct ccttctcgcg cttcaacgtg gcggacagcg cggtgacgat cggggtcgcg
    22381 ctgatgctcg tcaccgcgct gctagacggc cggcgcccgc ctgcggcgaa agcacgggcg
    22441 gcgagcgatg ccgattcgtc tcaaatgtag atctgctcat gcctgttcac atcatcacgc
    22501 tgcacgccga tacgcgcgag cggctggaca agtttgtcgc cgcgcgcgtg agcgacctct
    22561 cgcgcacggc ggtccagcgc ctgatcgacg acggcctcgt caccgtcaac ggcgcggtga
    22621 ccggcgccgc tcacaaggtg gggcaggggg atgaaatcgt cgtgcgcgtc ccgccgcctg
    22681 cgccggcgac gattgaagcg gaagacatcc ctctctcgat cgtctacgaa gacgccgacc
    22741 tgatcgtgat cgacaagccg gccggcttgg tcgtgcatcc ggcagcgggg cacgatcgcg
    22801 gcacgctcgt caatgccatc ctggcgcacg cgcccgatct gaagggcgtc ggcggagaga
    22861 tgcgccccgg catcgtgcac cggctcgaca aagacacctc gggcttgatc gtcgccgcca
    22921 agcacgacgc cgctcaccgc gagctgcagc gccagttcaa gtcacgccag gtcaagaagg
    22981 tgtacctggc cctagtcgag ggacgcctcg cgccgcgcgt aggcatcgtc gacgccccga
    23041 tcgcgcgcga tcgcgtccat cgcaagcgta tggcggtgtc ggcggacggc cgcccatcgc
    23101 gcacccggta caaagtgatt agatatttga gattggccga agcggcatac tcatttgtcg
    23161 aggcgcatcc cgaaaccggg cgcacacacc agattcgtgt tcacctcgcc tggctcgggc
    23221 atcccctcgt cggcgatacg ctgtacggcc gccggaagcc ggcgctcccg attgggcggc
    23281 aattcctgca cgcagcgcgc ttgacgctga gtctgccctc gaccggcgag gagcgcacat
    23341 tcgaatcgcc cctgccagac gatctggaac gcgtgctgag atcgctgggg gattattctt
    23401 gacaccgccc tgctaatccg cgataatccg ggtaccacag acttgtttac caccaagaca
    23461 cgaaggcaca aggtactccc attcttcgtg tctttgtgtc ttcgtggtga aggagaaccc
    23521 atgcgcgaag tgacgatcgc ctgcgtccag atgcaggtcg agctgaaccg gatggaagac
    23581 aacctcgtca agatgtcgga gtggatcgag aaagtcgccg tcgcgcagaa agtcgatctg
    23641 attgtcttcc ccgagctggc gacgaccggc tacgagtgcg gcgtgcgctt caccgacttc
    23701 gcgcagcgcg tgcccgggcc gtcggccaac ctgatcgcgc agcgcgccag cgagttcggc
    23761 gtgtacgtcg ccttcggctt gccgtctaag gagaaggtcg agtcggtgct gtacaactcg
    23821 gcggtgctcg tcgggccgga cggcgatctc gtcggcgaat accgcaaggt tcacttgaaa
    23881 ggcgaagagc ggctcgcctt ccggaacggg tttcgtttcg ccgtgctcga aaccgatttc
    23941 ggcaacgtcg ggctgatgat cggctgggac gtcgcctttc ccgaagcggc gcggacgtac
    24001 gcgctcgacg gcgcggacct gctgatcgtc tgcgcgaact gggagaagcc gaacgtcgaa
    24061 gagtggcgca cgtacgtcac ggcgcgcgcg ctggaaaact cgatcttcgt ggcggcgtcc
    24121 aaccgcatcg ggcaggacgc gacgctgtcg ttctgcgggg agacgatgat cgtcggcccg
    24181 cgcgggcaga ccttcgcgca agtcgacgaa gagatcgaag gctacgcgct ggcgcgcatc
    24241 gatctggacg acgtgcggcg ctatcgcgaa gagcttcagc agatccagaa ccgccagccg
    24301 agcgcctatc gcgccgtcgt gcggcaatac tgagaggaag gtagggcgag ttccttgagt
    24361 tccctgaatt ccacttggtg aagattaagg aactgaagga actcaag
//
LOCUS       DBNHXB010000255         9989 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3251073, whole genome shotgun sequence.
ACCESSION   DBNHXB010000255 DBNHXB010000000
VERSION     DBNHXB010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9989
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3251073"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="AC162G_10505"
     CDS             complement(<1..228)
                     /locus_tag="AC162G_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015413098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076292.1"
                     /translation="MKTGLTWSSRWILGGALALAVVALLAVVLVPRLRGPEQVSTPTY
                     WPTQGWRSSTPEEQGFDSAKLAEGLLAIRERN"
     gene            complement(225..698)
                     /locus_tag="AC162G_10510"
     CDS             complement(225..698)
                     /locus_tag="AC162G_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5076293.1"
                     /translation="MEHIMPAPVSPSSCTPHRPRVLIVDDMPQVRQELRLLLSLTGEV
                     EVVGEAANGFEAIGQAEALRPDAVVIDLEMPVMDGYEASRQIKTRWPACRVIALTVHD
                     GEAERQRAAQVGVDAFIVKGAPVEALVKAIIEQNEHQYGQAGALPPLQEHTGGAS"
     gene            complement(869..1675)
                     /locus_tag="AC162G_10515"
     CDS             complement(869..1675)
                     /locus_tag="AC162G_10515"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019350.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5076294.1"
                     /translation="MNPSRKPLKPGRTAVIAVYLAFAAGVARTLASTPSQDLWPWYLG
                     LHGVWLILFTAVLWRPATRSELLHLYFALQSAIVLALLSLNPELDIVTALFDLLCYQA
                     ALVFTGLPRWMWVGLFVLLTNGSLMFYLGPLEGLALGLMPSAAGVVLAAYVAVNEEIE
                     RARARSQVMLGELQETHRQLQAYADQVEELAAMEERNRLARELHDSVSQTMFSIVLNT
                     RSTQILLERNPARVRPQLEQLQELTQNALAQMRSLIAQLRPKVPEGLQDL"
     gene            complement(1786..2457)
                     /locus_tag="AC162G_10520"
     CDS             complement(1786..2457)
                     /locus_tag="AC162G_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5076295.1"
                     /translation="MMAPEDKIRVLIVDDHRVVRQGLRTFLELHDDVVVVGEAGDGLT
                     AVDMIRQLVPDVVLMDLVMPELDGIAATRQVKSLGGRTKVIALTSFTEDDKVFPAIQA
                     GASSYLLKDVSPDDLVEAIRAVHRGEARLHPDIVRRLMEQVAHQTGPIRETPAPAGGL
                     TERECEVIRWVAQGCSNHEIAQKLVISDKTVKSHVSNILSKLNLEDRTQLAIYAIKHG
                     LADSD"
     gene            complement(2454..3620)
                     /locus_tag="AC162G_10525"
     CDS             complement(2454..3620)
                     /locus_tag="AC162G_10525"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5076296.1"
                     /translation="MVWIFYLTAGLLFGAAMLRSLLYYGGTPVISQVLGLLMVWLVLL
                     MASETAISRRWSRYFPIYLILQSALIFALLLMPDASDFFAVLFWILSMQIMRRFSPSQ
                     GAVWIGLFTPLMTLPLVRTYGLPKGMAFALAYTAGNVLSAAYAFATQRAQTARAQNHI
                     LAQELQEANRQLQAHSAQLEQLAVARERHRLARELHDSVTQTIFSMTLTTQSAVLLMD
                     RDPGRVGAQLDRLGQLARSALSEMRVLISELRPGKAAEGGLIAALRRHLADGHLPENL
                     AVSLEVAGDQLLAPAEEQSLFRIAQEALNNIVKHARASQAQIRLHLAEPFWMEIQDQG
                     QGFDLQRARISGRVGLVSMGERAAEIGWRLQVITSPGAGTRVRVEKKPSEGRQP"
     gene            3966..4982
                     /locus_tag="AC162G_10530"
     CDS             3966..4982
                     /locus_tag="AC162G_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifH"
                     /protein_id="HIR5076297.1"
                     /translation="MAWQAQLKCNSLPWLLETESPGVRYLALRDVVGLPKDNPELRAA
                     RKSAHKQGPIAAILAEMDKAGHWARPGPGYNPKYRSTVWSVIMLAQLGASMEEDKRIA
                     RACAYLLEHALTEGGHFTASGAPSGTADCLQGNLCWALVELGYDDARLETAFEWMARS
                     VTGEGIAPAADRQAAVRYYAGKCGPTFACGSNNKLPCAWGAVKVMLAFSRWPAKRRTP
                     VIKRAIKHGVEFLLGTDPAAADYPAGYSDKPSSNWWKFGFPIFYVTDLLQNVEALAGL
                     GYGDDPRLANALAIVRDKQDSQGRWPLEYGYAGKTWVDFGPKKQPNKWVTLRALRVLK
                     AVGP"
     gene            complement(5192..5956)
                     /locus_tag="AC162G_10535"
     CDS             complement(5192..5956)
                     /locus_tag="AC162G_10535"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076298.1"
                     /translation="MLQVFFVAFGKEWMEQRRSHRLLVVAVVLVAFGLSSPLLAKLTP
                     ELIRLVPEGEALVPLIPPPTVADAVAQYVKNLSQFGIVLALLMAMGAVAQEKDKGTAA
                     LMLVKPMPRGAFLLAKFAALAITFAISVALAGAAGYIYTLLLFEEPNVSGWLALNGLL
                     LLFVLVFVALTLLCSTLTRSQAAAGGLAFGALVILSAVGAIPAIGEYLPGQLIVWGVG
                     LTLGTGQASWPALWVSLGLIATALAGAWLIFRRQEL"
     gene            complement(5958..6896)
                     /locus_tag="AC162G_10540"
     CDS             complement(5958..6896)
                     /locus_tag="AC162G_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076299.1"
                     /translation="MTAIRTEGLQKVFGAVRALDGLTLTVEPGVVFGFLGPNGAGKTT
                     TIRLLTGLARPTGGRAWVAGVEIGADRRQVAGRIGHLPEEPTFYPWMTPREFLDYVGR
                     VFGLSSREREARVTELLDLAGLAEVGRRRTGGFSRGMRQRLGLAQALVNRPEVLFLDE
                     PVSALDPAGRKELLELIERLRGQCTVFMSTHILADVERVCDTVGIIARGRLIVEAPQS
                     DLLTRYAVPAFEVECDPGSEERVSQWAAALRETKWISAVTTDGSSARVVVTDVEIAKR
                     ELVASAVQAGLVLTRYEMVKPSLEDVFLRLVGEGGE"
     gene            complement(6897..7094)
                     /locus_tag="AC162G_10545"
     CDS             complement(6897..7094)
                     /locus_tag="AC162G_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019241758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HIR5076300.1"
                     /translation="MELVVPEWLILVIPIVILQLALVVVGLMDLARRERTKGPKWMWA
                     LIVLFIGVIGPVVYFVFGREE"
     gene            complement(7160..7867)
                     /locus_tag="AC162G_10550"
     CDS             complement(7160..7867)
                     /locus_tag="AC162G_10550"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HIR5076301.1"
                     /translation="MTNAMWKRLGLWAIWFFVVIIGSVFLHEVGHGVGATIDGIHIST
                     GFNKVGTPDKTPDDPDFRTGMPDGIWSGLLGPIVSWALAIVFTLALYQMRRPTPAAFL
                     VGATAVANGLLRAAPMTMFLIPALGGQLHVEDEVAWGIWMVTHVFRPDLSGVTLSEIP
                     PGLLLSYPAVWILPLFSLAISLACLIFAYRGIFKLEGARMSHWSVRLLFVALPVAAWL
                     AAPPVLGALDRVVRINW"
     gene            complement(8001..8843)
                     /locus_tag="AC162G_10555"
     CDS             complement(8001..8843)
                     /locus_tag="AC162G_10555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR5076302.1"
                     /translation="MFHDIKPAHGLVLVAAIVSNFVLITLFTMLYAGGMIQPIVDVSN
                     GLIHPTLILGLPLFVLVVGVVVVWLGRLTPRDAGLEWSRLPSGIAVGLIFWVVVQIIQ
                     LLVGLGGTGKLVLDPVWAEEGVAPILGLLIGMLLGTALPEEVIFRGFAVPQLYLKLRD
                     RMPERHTTNLVLAVLVSQVIFSLTHVPNLVLRRDVAWHQLPGPLLGVLLNGVLYALVY
                     LRTNNLFIAMAVHALGNAPTPLLKTTVETPSLVFVFTVLLLLTWPVLARQAALSRRTK
                     HVKG"
     gene            complement(9055..9927)
                     /locus_tag="AC162G_10560"
     CDS             complement(9055..9927)
                     /locus_tag="AC162G_10560"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR5076303.1"
                     /translation="MKGKSIIEVLIVFGLTTLLTWWLNEEFMRLQLDSEVRRWLGGAV
                     FITISLVVISLTRKPWAEYGLTRRDWRLHLDVGMLAFLASFITLGTLFALILLNVDYN
                     SWAGAVPLAVAAMVATAVLLAVLNRQDTKDPSGQRRRSKARTNLIVLGGLLLLPIGLS
                     ILMGRFSWAIVSTVAWQFIVSGFGEELRFRGYYQSRVNQEFGQPWQVLGVRFGPGLII
                     ASALFGLVHAFNTFNPLAGLYEPAWAWAFWTFFGGLFFGLIRERTNTLVAPTIAHGGP
                     DAVGEALGMLFGWR"
BASE COUNT         2126 a         3173 c         2930 g         1760 t
ORIGIN      
        1 gttccgttcg cgtatggcga gcagcccttc cgccagcttg gccgagtcaa acccttgttc
       61 ctccggcgtg ctgctccgcc agccctgggt gggccagtag gtcggtgtgg aaacctgttc
      121 aggcccacgc agccggggaa cgagcacgac tgccagcagc gctacaaccg ccagcgccag
      181 cgctcccccg agtatccatc gcgagctcca cgtcaatcct gttttcatga tgcacctcct
      241 gtgtgctctt gcagtggagg caacgccccc gcctgaccgt actgatgttc gttttgttct
      301 atgatcgctt tcaccaaagc ctccaccggt gcgcccttca cgatgaaagc gtccacgccg
      361 acttgagcag ctctctgccg ctccgcttcg ccatcatgga cagtcaaggc gatcacgcgg
      421 caggcaggcc agcgagtctt gatctggcgc gacgcttcgt aaccatccat gaccggcatc
      481 tccagatcga tcaccactgc gtccggacgc agcgcctcgg cttggccgat ggcctcgaag
      541 ccattcgcgg cttcgccgac aacttccacc tcaccggtca gcgagagtaa caggcgcagc
      601 tcctggcgca cctgaggcat gtcatcgacg attagcacac gaggacggtg gggagtgcag
      661 gaagaagggg aaaccggggc cggcatgatg tgctccatgc gctttcgcgc cgaatcttta
      721 cgcttgcatc ataccggttc tggcgcgcaa gcgcatcgag ccggcagagc atttctgcgc
      781 gaccccagat tgaattcgtt ttctattgcc ggagttagac ggctctacat ctcaagtcgt
      841 agggcggggg atggattaga gaaagacctc aaaggtcttg gagaccttca gggactttgg
      901 ggcgcaattg ggcaatcagg ctgcgcattt gtgccagagc attctgggtc agttcctgca
      961 attgctccaa ctggggtctg acacgggcag gatttcgctc caaaaggatt tgtgttgacc
     1021 gggtgttgag gacaatgcta aacatcgtct gcgagaccga atcgtgcagc tcgcgtgcga
     1081 gccggttgcg ctcctccatg gctgcgagtt cttccacctg atcggcatac gcctgcaatt
     1141 gccggtgagt ctcctgtaat tcgccgagca tcacctgact cctcgcgcga gcgcgctcga
     1201 tctcctcgtt aacggcgaca tacgccgcca gcacaacgcc ggccgcgctg ggcatcagtc
     1261 ccagggccag gccttccagc ggccccaggt agaacatgag cgatccgttg gtgagcaaga
     1321 cgaacaggcc gacccacatc cagcgcggca ggccggtgaa aaccagcgcc gcctgatagc
     1381 acagcaggtc gaagagcgcc gtcacaatgt caagttccgg attcagcgac agcagcgcca
     1441 gcacgatggc ggactggagg gcgaagtaca gatgcagcag ttcggagcgc gtggcgggcc
     1501 gccacaacac tgctgtgaac aagatgagcc acaccccatg cagccccagg taccagggcc
     1561 acaggtcttg cgagggtgtc gaagccaacg ttcgggcgac cccggcggcg aaagccaggt
     1621 agaccgcgat cacggccgtc cggccgggct tgagaggctt gcgagaaggg ttcattcgtg
     1681 ctgacacgga ttcggtcacg gatacacgga agcagtcgta agaggtgtca atccgatctg
     1741 acacggattc ggtcacggat gcacggaaag cagcggcaag aggtgtcaat ccgaatcagc
     1801 caggccgtgc ttgatggcgt agattgccag ttgcgtgcgg tcctccaggt tgagtttgct
     1861 caggatgttg ctgacatggc tcttgaccgt cttgtcactg atgaccagtt tctgagcaat
     1921 ctcgtggtta ctgcatccct gcgccaccca acgaatgacc tcgcactctc gttcggtgag
     1981 accacccgca ggggccggcg tctcgcgaat agggccggtc tgatgggcga cctgttccat
     2041 caatctgcgg acaatgtccg ggtgcaaccg tgcttcgccc cggtggacag cccggatcgc
     2101 ctccaccaga tcatcggggg agacgtcttt cagcaggtag ctggaagcgc ccgcctggat
     2161 ggccgggaag accttgtcgt cctcggtaaa actggtcagt gcaatcactt tggtgcggcc
     2221 gcccagcgac ttgacctggc gcgtggcggc aatcccgtcg agttcaggca tgacgagatc
     2281 catcaggacg acatccggca caagctggcg gatcatgtcc acggctgtca acccatcacc
     2341 cgcctcgccg accacgacga catcgtcgtg caattccagg aaggtccgca ggccctgtcg
     2401 gacgactcga tgatcgtcca cgatcaacac cctgattttg tcttccggtg ccatcatggc
     2461 tgcctccctt ctgatggctt cttctcaacc cggacgcgcg tgcccgcgcc gggagaagtg
     2521 atgacctgca agcgccatcc gatctctgcg gccctctcgc ccatgctgac caggccgacc
     2581 ctgccgctga tcctggctcg ctgtaaatcg aagccctggc cctgatcctg aatctccatc
     2641 caaaaaggct cagccaggtg tagccggatt tgcgcttgag aggctcgggc gtgcttgacg
     2701 atgttgttca gcgcctcctg cgcaatgcga aagaggctct gctcttcggc gggcgcgagg
     2761 agttgatccc ctgcgacttc caaagagacc gccaggttct ccggcaggtg accatccgcc
     2821 aggtggcggc ggagggcagc gatcaagccg ccttcggcgg ctttccccgg acgcagttcg
     2881 gaaatcagca cacgcatttc ggagagcgcg ctgcgtgcca attggcccaa ccggtccaac
     2941 tgcgcgccca cccggccggg atcacgatcc atcagcaaca cagccgactg tgtggtgagc
     3001 gtcatgctga agatcgtctg agtcaccgaa tcgtgcagct cgcgcgccag gcgatgacgc
     3061 tcgcgcgcca cagccaattg ttcaagctgt gccgaatgag cctgcagttg gcggttcgct
     3121 tcttgcagtt cttgcgccag gatgtggttc tgggcgcgcg ccgtctgagc gcgctgtgtg
     3181 gcgaaggcat aagcggccga caaaacgtta ccggcggtgt aggccaacgc aaaggccatg
     3241 cctttaggta gtccataggt tctcaccagg ggcagcgtca tcaatggggt aaacagtcca
     3301 atccacacgg ctccctggct ggggctgaag cgccgcatga tctgcatact gaggatccag
     3361 aacaggaccg caaagaagtc agaggcatct ggcatgagca ggagcgcgaa gatcagggcg
     3421 gactggagaa tcaggtagat cggaaaatac ctggaccacc tgcgggagat cgccgtttcg
     3481 ctggccatca gcaagactag ccagaccatc agaagaccca gcacctgact gattaccggg
     3541 gtgcccccat agtaaagcag cgaacgcagc atcgccgccc cgaacaggag accggcagtc
     3601 agatagaaga tccacaccat ttcgcttacc tgcgttttct caataccgca agcgagcaag
     3661 aagtcatctg gcccaacctc taaagtagac ccggactcct caccctgtca cgtaacggaa
     3721 atcatgtaac tgtgagcctt tcatcagggg attgcttttt gctggaattg ccatatactg
     3781 aggatggatc gcagtgaaca accgaatgcc tcggggagtg cacgcacacc gaggccactg
     3841 ccagggtgga gattgccttg gtgggagagg catatggcgc cgcccaccga tgtggtgtgc
     3901 gggcgttttg ttgtgtggtg tagggcagat cggaagggcg agttcgatct aaggagaggt
     3961 gacacatggc atggcaagca caactcaagt gcaactcatt gccgtggctg ctggagacgg
     4021 aatcaccagg cgtgcgttat ctggcgctgc gagacgtagt tggtcttcca aaggacaatc
     4081 ccgaactacg tgctgctcga aaatcggcac acaaacaagg gcctatcgca gccatcctgg
     4141 ccgagatgga taaggcaggg cattgggcca ggccgggtcc aggctacaac ccgaagtatc
     4201 ggtccaccgt ctggtcggtg atcatgcttg ctcagttggg cgcatctatg gaggaagaca
     4261 aacggatcgc ccgtgcttgc gcttacctct tggagcatgc cctcacggag ggtggacact
     4321 tcactgcatc cggggcgcct tctggcaccg cagactgtct acaaggcaac ctgtgttggg
     4381 cgttggtcga gctgggttat gatgacgctc ggctggagac ggccttcgag tggatggcgc
     4441 gcagtgtcac cggagagggc atcgcgccag cagcagaccg gcaggcggcc gtgcgctact
     4501 acgcgggcaa gtgcggcccc actttcgcgt gcgggtcgaa taacaagctg ccctgcgcct
     4561 ggggagccgt caaagtgatg ttagccttca gccgatggcc cgccaaacgg cgaactccgg
     4621 tgatcaagcg cgctatcaag cacggtgtcg agtttctgct cggcaccgat ccggcggcag
     4681 ccgattatcc ggcgggatac agcgataagc cgagttcaaa ctggtggaag ttcggcttcc
     4741 cgatcttcta tgtgacggac ttgctccaga atgtcgaagc actggccgga ctgggctacg
     4801 gcgatgatcc ccggctggcg aacgcactcg cgatcgtccg cgacaaacag gactcccaag
     4861 gccgttggcc gttggagtat ggctacgccg gcaagacgtg ggtggacttt ggaccaaaga
     4921 agcagcccaa caaatgggtg acgctgcgtg ccttacgcgt gctcaaagcg gtcgggccat
     4981 agcaccaaaa caatcgaatg ctgtcggttg ggcggcacac tcatttgcac gcacgtctca
     5041 tgacgcaggt cgaagtggca aaactctggt ctccccttat ccacaacagg gctatttcat
     5101 tgctcaaatc gtattgggca gggagcccgt cgagcaggcg cttcgcctga cgcttgggaa
     5161 aaacgtgaga accgttcttg ggacaaggaa atcagagctc ctgcctccga aagatcaacc
     5221 acgccccggc gagcgccgtc gcgatcaggc cgaggctcac ccataaggct ggccaggagg
     5281 cctggccggt tccgagcgtc aaccccacac cccacacgat caactgcccc ggcaggtatt
     5341 cgccgatcgc cggaatcgcg cccacggcgg acaggatgac caatgcacca aaggccagcc
     5401 cgccggcggc cgcctgcgat cgggtgagcg tgctgcacag cagcgtcagg gcgacgaaca
     5461 caagcacgaa cagcagcagc agcccgttga gcgccagcca gcccgacacg tttggttcct
     5521 cgaacaagag cagcgtgtag atgtagcccg ccgcgccggc cagcgcgacg ctgatcgcga
     5581 aggttatcgc tagcgccgcg aacttagcca acaagaacgc gccgcgcggc atcggcttga
     5641 ccagcatcag cgccgccgtg cctttgtctt tttcttgcgc caccgcgccc atcgccatca
     5701 gcaaggccag gacaatgccg aactggctga gattcttgac gtactgcgcc accgcgtcgg
     5761 cgactgtggg cggtgggatg agcgggacca gtgcctcgcc ctccggcacg agccggatca
     5821 gctccggcgt aagcttggcc agcaagggcg acgacagccc gaaggcgacg agcaccaccg
     5881 cgacgaccag caggcggtgc gaacgtcgct gctccatcca ttccttgccg aatgcgacga
     5941 aaaagacttg tagcatgtca ctcaccccct tcaccgacca gccgcagaaa gacatcttcc
     6001 agcgagggct tcaccatctc gtagcgagtg agcaccagcc cggcctgcac cgccgaagcg
     6061 accagctcgc gcttggcgat ctcgacatcg gtcacgacca cccgcgcgct agacccatcg
     6121 gtcgtcacgg cagagatcca tttcgtctcg cgcagcgcgg cggcccactg gctgacgcgc
     6181 tcttcggagc cgggatcgca ctcgacctcg aacgccggaa ccgcgtagcg ggtcagcaga
     6241 tcgctctgcg gtgcttcgac gatcagtcgt ccgcgcgcga tgatgcccac ggtgtcgcac
     6301 acgcgctcca cgtcggccag gatgtgcgtg gacataaaca cggtgcactg gccgcgcagg
     6361 cgctcgatca gctccagcag ttccttccgc ccggcggggt cgagcgcact gaccggctcg
     6421 tccaggaaca agacctccgg gcgattgacc agcgcctgcg cgagccccaa tcgctggcgc
     6481 attccacgcg agaacccgcc ggtgcgcctt cggccgactt cggccagccc ggccaggtcg
     6541 agcagctctg tcacgcgcgc ttctcgctcg cgcgacgata gtccaaagac gcgcccgacg
     6601 tagtcgagaa actcgcgcgg cgtcatccac ggatagaagg tcggctcttc gggcaagtgg
     6661 ccaatccggc cggcgacctg tcggcgatcg gcgccgattt ccacaccggc cacccaggcg
     6721 cgcccaccgg tcggccgggc caggccggtc agcaggcgga tagtcgtcgt cttgcccgca
     6781 ccgttggggc cgaggaatcc gaacaccacg cccggctcga ccgtgagcgt caggccatcc
     6841 agcgcgcgga ctgcgccgaa caccttctgc aacccttcgg tccgaatcgc cgtcatctac
     6901 tcctcccggc caaacacaaa gtatacgacc ggcccgatca cgccgatgaa caggacaatc
     6961 agcgcccaca tccacttggg gcctttcgtc cgctcgcgcc gtgccaaatc catcagcccg
     7021 acgacgacca gcgcgagctg caagatcacg atcgggatga ccaagatgag ccattctggc
     7081 actaccagtt ccatgtttta tccccctggc ggtttgacgg ttgagtcgcc ctgaccggcg
     7141 agatcgttct gcaaactcgt caccaattga tgcgaaccac tcgatcaagc gcgcccaata
     7201 ccggtggcgc ggcgagccac gcggcgaccg gaagcgcaac aaacagcaag cgcacactcc
     7261 aatgagacat cctcgcgccc tccagtttga agatgccgcg ataggcaaag atgaggcacg
     7321 cgagggaaat cgcgagtgag aaaagcggta gaatccacac ggccgggtac gacaacagca
     7381 agccgggggg aatctcactc agcgtgactc cgctgaggtc tgggcgaaag acatgcgtca
     7441 ccatccagat gccccaggcg acttcgtcct ccacatgcag ttgtccacct agcgcgggga
     7501 tcaggaacat ggtcatcggc gctgcgcgca acaagccatt ggcaaccgcc gtcgcgccga
     7561 ccaggaaggc tgccggcgtc ggccgccgca tctgatacag ggcgagtgta aacacaatcg
     7621 ccagcgccca cgacacgatt gggccgagca ggccggacca aatgccatcc ggcatgcccg
     7681 tgcgaaaatc cggatcgtct ggtgttttgt ccggcgtccc caccttgtta aagcccgtgc
     7741 tgatgtgaat gccgtctatc gtcgcgccga cgccgtgtcc cacctcgtgc aagaacacgg
     7801 agccgatgat gacgacgaag aaccagatcg cccaaagtcc caatcgtttc cacattgcgt
     7861 tcgtcattct ggcgctccat gcagccgtta cggtttcacg cggcagcttt cggaacgtgc
     7921 agcgcgatga taatcagcac gaattacatg acgatcagcg ccaggatcag cggcagccat
     7981 tggttcagga tttccatcgt tcatcccttc acatgttttg tgcggcgcga aagagccgcc
     8041 tgacgcgcaa gaaccggcca cgtcagtaac agcaacacgg tgaaaacgaa caccagggat
     8101 ggtgtttcaa cggttgtctt cagcagcggc gtgggcgcat tgcccagggc atgaacggcc
     8161 atggcaatga acaggttgtt ggttctcaga tagacgagag catagagcac accgttcaat
     8221 agcacaccca gcagcgggcc tggcaactga tgccatgcca cgtctctgcg cagaacgaga
     8281 ttaggaacgt gagtcaggga gaaaatcact tgggagacga gaacagccag caccaggttc
     8341 gtggtgtggc gctccggcat ccgatcgcgc agtttcaggt atagctgcgg cacagcgaaa
     8401 ccacgaaaga tgacctcttc gggtagggct gtgccaagca gcatgcctat caataagccc
     8461 aggatcgggg ccacgccctc ttccgcccaa accggatcca gcacgagttt gcccgtgcca
     8521 cccagcccta ccaatagttg gatgatctgc acgacgaccc agaagatcag gcccaccgcg
     8581 ataccgctgg gcaggcgaga ccactccagg cccgcatccc tcggcgtcag cctgcccaac
     8641 cagacgacga ccacgccgac gacaaggaca aacagcggca atcccagaat gagcgtcggg
     8701 tgaatcaacc cgttggacac atcgacaatc ggctgaatca tccctccggc atagagcatc
     8761 gtgaatagag tgatcaaaac gaaattggaa acaatcgctg ctacgagtac cagcccgtgg
     8821 gccggcttga tatcatggaa catgactctg cgccaccttg ttgctcttca gtgtgcctct
     8881 cacaacctcg aacccgcaaa tcggacgata tcgttctcga agctactcat caccggcatc
     8941 ccgacgccca gttcgcggcc ggtgttcaac aggtcgtagt aggccatatt ctcacgtcgc
     9001 ctccccatgg tttgcccgca tctcagtaca gactcggcga tgcactccgc gtggctacct
     9061 ccagccgaac aacatgccca gtgcctcacc caccgcatcc ggcccgccat gcgcgattgt
     9121 cggcgcgacc agggtgttcg ttctctcgcg tatcagcccg aaaaacagtc cgccgaaaaa
     9181 cgtccagaac gcccacgccc aggctggctc gtacaggccg gctaacggat tgaatgtgtt
     9241 gaaggcgtgc accaggccga acagtgccga cgcgatgatc aaccccggac cgaaccgcac
     9301 gcccaggact tgccacggct gcccgaattc ctgattgacg cgcgactgat agtagccccg
     9361 gaagcgcaat tcttccccga aaccggagac gatgaactgc cacgccacgg tcgaaacgat
     9421 ggcccacgag aaacgcccca tcaggatgct gagcccgatc ggcagcagaa gcaagccgcc
     9481 cagaacgatc aaattcgtgc gtgctttgga tcggcgccgc tggcccgacg ggtctttcgt
     9541 atcctgtcga ttcagcaccg ccagcaggac ggcggttgca accatcgcag caaccgccag
     9601 gggcacagcg ccggcccatg agttgtaatc gacgttgagc agtatcagcg cgaacaacgt
     9661 gccgagtgta atgaagctgg ccaggaatgc cagcatgcca acgtcgaggt gcaggcgcca
     9721 gtcacggcga gtcaatccgt actcggccca cggtttgcgc gtcaggctga tcacgaccaa
     9781 cgatatggtg atgaaaacgg caccacccag ccaacgccgc acttcagaat caagctgcag
     9841 ccgcatgaac tcttcgttca gccaccacgt caacagcgtg gtcaatccga aaacgatcag
     9901 gacttcgatg atagacttgc ctttcatcgt cacgctcctt atgccgctct aagctcggcc
     9961 ctccgatcaa gcaccggtct cacgccatc
//
LOCUS       DBNHXB010000256         4889 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3083452, whole genome shotgun sequence.
ACCESSION   DBNHXB010000256 DBNHXB010000000
VERSION     DBNHXB010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3083452"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="AC162G_10565"
     CDS             <1..767
                     /locus_tag="AC162G_10565"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5076304.1"
                     /translation="WPVVGTAAVGLLISASTLYLASMNMEGFTQSHAAEVGASALGMA
                     WPAFPPLALGLLPLAAAPAIALDRQYGVEELLRSLPLSGGMYLAGKILGTLAVVLLIG
                     AVMLALHLGLHLLLIGPVNPQLYLDLTLLSGLPLFLWAPAMGVAAACAARTRRVAVTI
                     GLMVGLFGVLVWGLLATPPASFLIEMNPVTAWIGQMMTHPPASDFVLARYGVLMQSGM
                     TAPTAWHVAGSLLVALLVLGVAWTLARLWLLWKENF"
     gene            767..1651
                     /locus_tag="AC162G_10570"
     CDS             767..1651
                     /locus_tag="AC162G_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076305.1"
                     /translation="MIKITNLTKTYAGNVQALRGIDLEIGAGMFGLIGPNGAGKTTLM
                     RILAGLLRPTGGSVRVLGHDVTTAKGKQAVKAALGYLPQELGLYPDLTGREFLDYIAI
                     LKGVTDSKARYRQVAELLELVGLSEAANRRMKTYSGGMKRRVGIAQALLGNPRLLIVD
                     EPTSGLDPEERVRLRNLLADLSRRCTVILSTHIVEDASQSCNDLAVLNQGCTVFRGNP
                     RDLIAQARGKVWTITTTGERPDAELTTVSTLQRQECVQYRVLGNPAPHYAAQAVEPTL
                     EDGYIWLMRHVHVHPTRG"
     gene            complement(1665..2321)
                     /locus_tag="AC162G_10575"
     CDS             complement(1665..2321)
                     /locus_tag="AC162G_10575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR5076306.1"
                     /translation="MLQNNKLIIVVEILAVAFFPLVLALVQVSRTIIPLLLVGWLSLW
                     LRRKSWKEIGFHRPPSWPKVVLIGGGIAVAGVCLSEKAISPFLLRLTGETQPQVAEFV
                     LLRGNAFYFLFLLIGIWLLAAIGEELVYRGYVLNRLVDLFGSGKLGWGIGLIVSSLMF
                     SLGHGLFNLAVIIGGFILGLIEGGLYLAGHRNLWFSVVFHGTWDTIFLTLFFLGLSLR
                     "
     gene            complement(2538..4760)
                     /locus_tag="AC162G_10580"
     CDS             complement(2538..4760)
                     /locus_tag="AC162G_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5076307.1"
                     /translation="MRRASPYKAKRRAVRRPAERLPPSVDRRRALKAFVESLRWLQPI
                     DEVARQIVQRVAGEAGAAWCGLLLRDDARARLSLTRFAGSAPDPITSIPLDDARLAIL
                     GRQNRPFRVPNTVLWNDWRRRRIVLALPLIQNGRWIGLILIGERPAAWTRADLSLLNA
                     LRRNAGVAIENARRYSAAIQSRNDLAAVFETTLVASSLLDLQDALKQLAEHMVQAVVV
                     DGVTFSAWERERDCLLNWIGYRRKPFGPPDALGSVQPLAELPIAREALLDRRPRILSS
                     LHVQSHPLEAQWLNEHGIAQMLLLPLVARDMVIGLARLYTLDLSRSFEPHDVQLAQTL
                     AAQAALAIDNAHLIEELRDAARTLERKVAERTEELERTLWRQQVEASKTQAIIESVSD
                     GVVVVDSSGTLILVNAAAEGILGRSREQMLGRQLSGLAAALGEPALPWVRAMQEWLGG
                     DSTHEERIELGERTISVILSPVSLGGEAQGLVNVLRDVSRDVEVNRAKSAFVSTVSHE
                     LRTPMTAMMGYVDIILKGMAGPIGEEQRRFLQIVRNNIDRLTVLVNDLLDLSHIETGR
                     LRLTFGPVSLHDTVAEVLRTLHARVDEKQHTLEARVSPALPPVRADGSRLTQILINLV
                     GNAIAYTPPGGHITISARADDRFAVVEVSDTGVGIAPEDQRRVFERFFRADNATVREA
                     KGTGLGLPIVQSLVEMHGGRIWVHSEGVPGKGSTFSFTLPLWETDAPVAKPLVAAETQ
                     "
BASE COUNT          939 a         1513 c         1558 g          879 t
ORIGIN      
        1 gctggccggt agtcggcacg gcggcggtag gactgttgat cagcgcatcc accttatacc
       61 tggcatccat gaatatggaa ggattcacgc agagccacgc ggccgaagtt ggcgcaagtg
      121 cgctggggat ggcctggccg gccttcccac ccctggcact cggcctgctg ccgctcgctg
      181 ccgcaccggc gatcgcactc gatcggcaat atggagttga agaattgttg cgcagcctgc
      241 cgttgagcgg gggtatgtat ctggcgggca aaatcctggg cacactcgcg gtcgtgctat
      301 tgatcggcgc tgtgatgctg gcattgcacc tcgggctgca cttgctgctc atcggcccgg
      361 tcaatccgca gctatatctg gacttgaccc tcttgagcgg gttacctcta ttcctgtggg
      421 cgccggcgat gggtgtcgcg gctgcctgtg cggcgcgcac acgccgggtg gctgtgacga
      481 tcggtctgat ggtgggactg tttggcgtgt tggtgtgggg tttactcgcc acaccgccgg
      541 cttcgtttct catcgaaatg aatcccgtga ccgcctggat cggtcagatg atgactcacc
      601 cacctgcgtc cgactttgtg ctggcgcgct acggggtatt gatgcaaagc gggatgacgg
      661 cgccaacggc gtggcacgtc gccggatcgc tgctggtggc gttgctcgtg ctgggggtgg
      721 cttggaccct ggcccggctg tggttattgt ggaaggagaa cttctgatga tcaagattac
      781 gaacctgacc aagacgtatg ccggcaacgt gcaggcactg cgcggcatcg acctggaaat
      841 cggcgccggc atgttcggcc tgatcggccc caacggcgcg ggtaagacga cgctgatgcg
      901 catcctggcc ggcctgctgc gcccaaccgg cggcagcgtg cgcgtgctcg gtcatgacgt
      961 gaccacggcc aaaggcaaac aagcggtcaa ggcagccttg ggatacctcc cgcaggagtt
     1021 ggggctgtac cccgacctga ccggtcgcga gttcctcgac tacatcgcca tcctcaaagg
     1081 cgtgacggac agtaaggccc gttatcgtca ggtggctgaa ctgttggagc tggtgggatt
     1141 atcggaggcc gccaaccgcc ggatgaagac ctattccggg gggatgaaac gccgtgtcgg
     1201 tattgctcaa gcgctcttgg gcaatcccag actgttaatc gttgacgaac cgacctcggg
     1261 acttgacccc gaagagcgcg tgcgcctgcg caatttgttg gccgacctgt ccagacgctg
     1321 caccgtgatc ttgtcaacgc acatcgtcga agatgccagc cagagctgca acgatttggc
     1381 ggtgctcaac cagggttgca cagtgtttcg tgggaatcct cgcgacctga tcgcgcaggc
     1441 gcgtggaaag gtgtggacga ttaccacgac gggcgagagg cccgatgctg agttgacgac
     1501 ggtctccact ttgcagcgac aggagtgtgt tcaataccgg gtgttgggca atccggcgcc
     1561 acactatgcc gcccaagcag tcgagcctac ccttgaagac ggttatatct ggctgatgcg
     1621 ccatgttcac gtacatccaa cacgaggttg acactggggt atggtcaacg caaggataaa
     1681 cctagaaaaa acaatgtgag gaagatcgta tcccacgtac cgtgaaagac aacggaaaac
     1741 cacaagttcc gatggccggc cagatacagt cccccttcaa tcaggcccaa aatgaaaccg
     1801 cctatgatga cggccaggtt gaataggcca tgacctaaac tgaacatgag tgaactcacg
     1861 atcagcccga ttccccatcc aagcttgcca gaaccgaaaa ggtcaaccag acgattcaag
     1921 acataccccc gatagactag ttcttcccct atcgctgcca gcagccatat tccaatcaag
     1981 aggaacagga agtaaaaggc attgccccga agcagaacga attcggcgac ttgaggctgc
     2041 gtctcacctg tcaagcgcag tagaaaaggc gagattgctt tttcactgag gcacacacct
     2101 gccacggcaa ttccgccgcc gataagaacg actttgggcc agcttggggg acgatgaaag
     2161 cctatttcct tccagctctt acgacgcaac caaagcgata gccatcctac aaggaggagg
     2221 ggaataatcg tccttgatac ctgtacaagt gccaggacca gtgggaaaaa agctactgcc
     2281 aatatctcca caacgatgat cagtttattg ttctgaagca aacctccaat ttgatcacgg
     2341 acagaagaca gctgtgagtt aacgaaccga aacatattgg agtgcgggct ttagcccgca
     2401 tctactgagc gacgtagggc ggctttcgat agccgccttc aaggctgagt ctcagcagat
     2461 ccaaagttgc cagcggaggc ggctcccatg ccgccgattc gcgggcgtgg gagcccgctc
     2521 tgcttccgaa tctgggtcta ctgagtctca gcggcgacca gcggtttcgc cacgggcgca
     2581 tccgtttccc acaacggcag cgtgaacgag aaagtgctgc ctttaccggg cacgccttcg
     2641 ctgtgcaccc agatgcgccc gccgtgcatc tcgaccagcg actggacgat cggcaggccc
     2701 aggcccgtgc ctttcgcctc gcgcacggtg gcattgtccg cgcggaagaa gcgctcgaac
     2761 actcgccgct gatcctccgg cgcaatgccc acgcccgtgt cggacacttc gacgacggcg
     2821 aagcgatcgt cggcgcgcgc cgagatcgtg atatgcccgc cgggcggcgt gtaggcgatc
     2881 gcgttgccga ccagattgat cagtatttgc gtcaggcggc tgccgtcggc gcgaacgggc
     2941 ggaagcgccg gcgagacccg cgcctccaaa gtgtgctgct tctcgtcgac gcgcgcgtgc
     3001 agcgtgcgca gcacctcggc gacggtatcg tgcagcgaga ccggcccgaa ggtcaggcgc
     3061 aggcggccgg tctcgatgtg gctgaggtcg agcagatcgt tgaccaggac cgtgagccgg
     3121 tcgatgttgt tgcgcacgat ctgcaagaac cggcgctgct cctcgccgat cgggccggcc
     3181 attcccttca aaatgatgtc gacgtagccc atcatcgccg tcatcggcgt gcgcagctcg
     3241 tgcgagacgg tcgacacgaa ggcgctcttg gcgcgattga cctccacgtc gcggctgacg
     3301 tcgcgcaaga cgttcaccag gccctgcgct tccccgccca ggctgacggg cgagaggatg
     3361 acgctgatgg tgcgctcgcc cagctcgatg cgctcttcgt gcgtgctgtc gccgcctaac
     3421 cactcctgca tggccctgac ccaggggagc gccggctccc ccagcgcggc cgccaggccg
     3481 gacaactgcc ggcccagcat ttgctcgcgc gagcgtccga ggatgccctc cgcagccgcg
     3541 ttcaccagga tgagcgtgcc cgacgaatcg acgacgacca cgccatcgct cacggactcg
     3601 atgatggcct gcgtcttgga ggcttcgacc tgttggcgcc agagggtgcg ctccagctcc
     3661 tcggtgcgct cggccacctt gcgctcgagc gtgcgcgccg cgtcgcgcag ctcctcgatc
     3721 aggtgcgcgt tgtcgatagc cagcgccgcc tgcgcggcaa gcgtctgcgc cagttgcacg
     3781 tcgtgaggct cgaaagacct cgacagatcg agcgtataga gccgggccag cccgatgacc
     3841 atatcgcgcg cgacgagcgg cagcagcagc atctgcgcga ttccatgctc gttcagccac
     3901 tgcgcctcga gcggatggga ttggacgtgg agcgaagaga gaatgcgcgg ccgccgatcg
     3961 aggagcgcct cgcgcgcgat gggaagctcg gccagcggct ggaccgagcc taaggcgtcg
     4021 ggcgggccga agggtttgcg ccggtaaccg atccagttga gcaaacagtc gcgctcgcgt
     4081 tcccaggcgg aaaacgtgac gccgtcgacg acgacggctt gcaccatgtg ctcggccaac
     4141 tgcttgagcg cgtcttgcag gtcgagcagc gaagaggcga cgagcgtcgt ctcgaagacg
     4201 gcagccaggt cattccggct ctgaatcgcc gcgctgtagc gccgcgcatt ttcgatggcg
     4261 acccccgcgt tccggcgcag cgcgttgagc agcgacaggt ctgcgcgcgt ccaggcggcg
     4321 ggccgctcgc cgatgaggat caggccgatc cagcgaccgt tctggatcag gggcagcgcc
     4381 agcacaatgc gccgtcgtcg ccagtcgttc cacagaaccg tgttcggaac ccggaaaggt
     4441 cgattctgcc ggccgaggat cgccaggcgg gcatcgtcga gcgggatcga agtgatggga
     4501 tcgggggcgc tccccgcgaa gcgcgtcagg ctgaggcggg cgcgagcgtc gtcgcgcagc
     4561 agcagaccgc accacgcggc gccggcctcg ccggccacgc gctgcacgat ctggcgcgcg
     4621 acttcgtcga tcggctgcag ccagcgcagc gattcgacaa aggctttgag cgcgcggcgc
     4681 cggtcgacgg acggcggcag ccgctccgcc ggcctgcgta ccgcccggcg tttcgctttg
     4741 tagggcgatg ctcggcgcac gaacgacctt ccatgagcct gtgtgtcaac gtagggcgcg
     4801 tttccatacg cgccgcaaat ctcgatcgag gcgcgaatga agacgatcga gggggtttat
     4861 atcatcaaac ccgacgccgc gcaaccgct
//
LOCUS       DBNHXB010000257        10299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3922654, whole genome shotgun sequence.
ACCESSION   DBNHXB010000257 DBNHXB010000000
VERSION     DBNHXB010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10299
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3922654"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..787
                     /locus_tag="AC162G_10585"
     CDS             143..787
                     /locus_tag="AC162G_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HIR5076308.1"
                     /translation="MLNSLLGRREQEDVVRKMGRLPPGQSLTQKFPVLHYGPVPPFNP
                     ATWDFRVFGLVEEEKRWTWEEFNQLPRRKIAMDIHCVTRWSKFDTEWEGVHIQDLIDQ
                     GTIKPKPEARFIMQHCEYGFTVNIPIELALGDQFIMATHFNGQPLDAEHGYPLRGVAG
                     ALPGKPMDANDRYFWKGGKWLRGLEFIAKDRPGFWEQAGYSMTANIWKEERFGW"
     gene            complement(820..2145)
                     /locus_tag="AC162G_10590"
     CDS             complement(820..2145)
                     /locus_tag="AC162G_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012783.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR5076309.1"
                     /translation="MKRRMLGLLICSFLTVLLATVTAAAQDEAPRVYRIVLQGPLTPV
                     WATHIERSIAAAERDGAEALLIVLDTPGGQINLMQQLVEIMRASDVPLVVYVSPRGAA
                     AASAGTLITLAAHVAAMAPETTIGAASPVGGEGEDIGETLEQKVKQDIRALARALTDR
                     RGEQATSLAEAMIEEAKAVHAHEALAAALIDFVAVDEPDLLRQLDGFTVEVANRPVML
                     NTTGAQIQTLEMSWFEQLLNRVIDPNILAILLFIGIQAILIELSNPGGWVAGFIGVVC
                     LALALYGLGVIPVNWFGLALIVIAFALFIYDLTTPTHGVLTTVAAGTLVAGLLVLFSE
                     PGAEQFGRLSLPLAIGLGAGAAAFFGFVLWKGLRAQRAKPAFGGDSLIGSTGVARSDL
                     TPQGFVLVEGERWQAVGEDGEVKEGTPVQVVGRDGLRLSVRRVQPGTEK"
     gene            2269..3051
                     /locus_tag="AC162G_10595"
     CDS             2269..3051
                     /locus_tag="AC162G_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR5076310.1"
                     /translation="MLDALFSSGGLVLLSLIILLIVILTAAIKILPEWERGVILRLGR
                     LSSVKGPGLVVIIPFIDRLIRVSIRVVTLDVPQQRVITLDNVTIEVDAVVFYRVVDPA
                     KSVVAVEDFRRASFFVTQTTLRNVIGQSEMDELLARRERINEKLQVIIDEATEPWGVK
                     VGAVEVRDVRLPDTMQRAMAAQAEAERVKRAKVIHAEGEYQAAQRLAEAAGRMAEQPA
                     ALQLRYLQTLTEIAAEKNSTIIFPLPLEYLKGFERLLAERPK"
     gene            complement(3153..4523)
                     /locus_tag="AC162G_10600"
     CDS             complement(3153..4523)
                     /locus_tag="AC162G_10600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR5076311.1"
                     /translation="MDNPIAPSENAEGVMQSAVASDASAPPSTSPGGWSPRARRRLSL
                     TIAAGVISALAGFVLLVAPLGSSDEQLRLQSVAGAASIVAGGLIAARARRLRSASGPH
                     RPYAPRDPRPARATFVWVWLVGGGIGAVISGRLIGVGPAMLVATSIGFAAAGAGAVWI
                     SRWIGVKVERAWPPAELSQAIRLRVHEWAVFVAFAWGMFSTFPSLVLEATFGGLLGQA
                     TLGESPLDLVSESGLPNVIVDPELLAWLLVSFVFIAPPIEEASKAIGLRFVRAEIQST
                     TGGLLLGMSIGLGFGFLESAFFILEGVGSFAVLVLMWVRLATLVAHCVTTGLTGAGYA
                     RARLSGDRRALWRGLGRAAIVHAAWNALAVGALPLLTVLGPGEFIACCLVLIYMPLLI
                     VAGLRVLPRVVMAEIHQSIQDDHALAGVPLPREWSPIEEGLWWRIAGGRPQHPSPATG
                     DTPAPA"
     gene            complement(4527..6998)
                     /locus_tag="AC162G_10605"
     CDS             complement(4527..6998)
                     /locus_tag="AC162G_10605"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HIR5076312.1"
                     /translation="MAIKKLTAKQARRACPPSRFTFRTTAELPAAADIFGQPRATRAI
                     DFGIDVGGPGFNIFVLGPGGSGRTTTIERFLAEKAIREKVPGDWVYVHNFVDPYKPRA
                     MRLPPGRANAFRQDMQSLVGHLETDVPRAFESEEYSQARARISRELDEKQQAAYHELE
                     SAAAQRNYAIVRTPGGLLIAPLVDGKPAPIEALAGLPPDQRQQMEQTGMDLQQILADS
                     VRKVRIYEKEAQEAVAELDRQVAANVAGHVVDALIERYQTDCPEVVEYLEEVRRDIID
                     HIGEFSPSQQQQPQPPDPLRGPSARLRYQVNVLVDHARTLGAPVVNERNPTFMNLIGR
                     IERDVRFGGTVMDFTMLRSGALHRANGGYLVLRAKDVLNDPIGWAALKRALDSGQVQI
                     DDPGTQFQMFSTRTLEPEPIPLDVKVIMLGSPSLYYLLYAVDEDFQKLFKVKADFAPE
                     MDRTPENEHAYALFIRARCDEEGLCALDRGAVANIVEYGSWLAEDQEKLSTRFGEVAN
                     VVREAAYWASKAGRDVATADDVRTAIRESRQRADQIEQQSRRLILDGTITIATQGAIV
                     GQINGLSVRIAGDHAFALPSRISARTYLGRAGVINIEREVKLSGRIHDKGVLILQGYL
                     GSQYAVERPLTLSASLVFEQSYSDVDGDSASSTELYALLSSLSGLPIRQDIAATGSVD
                     QLGRVQAIGGVTHKVEGFFDVCHARGLTGEQGVMIPAANVKNLMLREDVVEAIDEGRF
                     HIWAISTVDEGIELLTGAKAGKRGAKGKFPPDSVHGRVEARLKEIAERMEKAGKPAED
                     KGNSQSSHEPNGERPDAPKRRRKTK"
     gene            complement(7120..8256)
                     /gene="dnaN"
                     /locus_tag="AC162G_10610"
     CDS             complement(7120..8256)
                     /gene="dnaN"
                     /locus_tag="AC162G_10610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR5076313.1"
                     /translation="MRVSCLQENLAKGLSIVGRAVAPRSTLPVLGNVLLATDNGRLRL
                     AATNLEIGITCWIGAKVEEDGAITVPARTFIDLVNALPQDRVDMELAVRTQTLNVRSG
                     RFENNIKGIDAQEFPIIPSFEEDSAIQIAPDALKKMIEQVVFAAATDESRPILTGVLA
                     KFSGDKLVFAAADGFRLSVRAAPLLTPVSKPIEVIIPARALAELGRVSGEQKDPISIL
                     FPPQRSQVLFHLSNVELVSQLIDGKFPDYSAIVPKRYETRAVMDTAPLLKACRAASVF
                     ARENSNIARVTIAPGSELAPGHVTVQATSAETGDNAGELDASVEGAQVEIAFNVKFMI
                     DVLSVAGTPQIALETTSPSSPGMIRPIGDDAFTHVIMPMHIGGR"
     gene            complement(8374..9261)
                     /locus_tag="AC162G_10615"
     CDS             complement(8374..9261)
                     /locus_tag="AC162G_10615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5076314.1"
                     /translation="MTQSPPVIDYEGSKYSTEFWTPQREYEDRAERIALRAMLPPRGR
                     RLVEIGAGAGRLAELYLGYDEIVLMDYARSTLLEARARWGRDPRFTFVAADVYRLPFA
                     DTAFDTLVMVRVLHHLVDVPKALGQIAAALRAGGTFVVEFANKRNLKAMLRYALRRQP
                     WSPYSREPVEFVELNFDFHPAWMQAQLRAAGFNIRQNRSVSFFRVGFLKRLLGAKTLA
                     SIDGALQRPLAPLALAPSQFLLCTLRRAGATDRAIGLFKCPECGSTEWREAPDALTCA
                     NGHAWPVREGVYDFRATDE"
     gene            complement(9258..9644)
                     /locus_tag="AC162G_10620"
     CDS             complement(9258..9644)
                     /locus_tag="AC162G_10620"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR5076315.1"
                     /translation="MAGHLLDSGLVIRHFRGDPEAVHLLRELGRRTRLGVSVVTHLEV
                     YAGMHPHEAYHTRKLLSRFAAYDVDREIAERAGDFLRRHHKDGTSIPDAIIAATAVRH
                     GLMLVTLNAKHFPMPELSLYASSVDG"
     gene            complement(9648..9941)
                     /locus_tag="AC162G_10625"
     CDS             complement(9648..9941)
                     /locus_tag="AC162G_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076316.1"
                     /translation="MTRASNTTQKITVTLPKALIQRLRKQVPARKRDEFVAQALEERL
                     VVAEQAAVLEETAGAWKDTDHPDMRTPEDIDRWLSNLRGTWPERLTQLQARRM"
     gene            complement(10018..>10299)
                     /locus_tag="AC162G_10630"
     CDS             complement(10018..>10299)
                     /locus_tag="AC162G_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076317.1"
                     /translation="IMLAHTWSGDAASAQEAFDRLSADFPAGAPGHEYQELAQVFWSK
                     YAETNRLSQACLAANAHANNETDVLDGLNAFGYANRQYVAADMCPFVGP"
BASE COUNT         1909 a         3361 c         3212 g         1817 t
ORIGIN      
        1 agagcgcggc cttcgcgaga ctagaatgtg gtggatgaag gcgggcgacc gccagggtcg
       61 cccctacatt tgaggatagg ctgttaatcc ccgcgtaagg gtctgagata taattgagtt
      121 agaaaacgtc agggaggatg ccatgctgaa cagcctgttg ggccgccgcg agcaggagga
      181 cgtcgtgcgc aagatgggtc gcctgcctcc cggccagtcg ctcacacaga aattcccggt
      241 gctgcactac gggccggtgc cgccattcaa cccggcgacg tgggactttc gcgtcttcgg
      301 tctggtcgaa gaagagaagc gctggacgtg ggaggagttc aatcaactgc cccgccgcaa
      361 gatcgccatg gacattcact gcgtcacgcg ctggagcaag ttcgacacag agtgggaggg
      421 cgtccacatc caggacttga tcgaccaggg caccatcaag cccaaacccg aagccaggtt
      481 catcatgcag cactgcgaat acggcttcac cgtgaatatt cccatcgagc tggcgctggg
      541 cgaccagttc atcatggcga cgcacttcaa cggccagccg ctcgacgccg agcacggcta
      601 tccgctgcgc ggcgtggccg gggcgctgcc cggcaagccc atggacgcga acgatcggta
      661 cttctggaag ggaggcaaat ggctgcgcgg gctggagttc atagccaagg accgtcccgg
      721 cttctgggaa caggccggct actcgatgac agcaaatatc tggaaggaag aacgattcgg
      781 gtggtgaaca cgaagtgacc ggcacctttg aggtgccggt cacttctctg tcccaggctg
      841 cacacgccgc acggagagcc gcaagccatc tctgcctaca acctgaaccg gcgtaccctc
      901 tttgacctcg ccgtcttcgc cgaccgcctg ccagcgctcg ccttcgacga gcacgaaacc
      961 ctgcggcgtg agatcgctgc gcgccacgcc ggtcgacccg atgagcgagt ccccgccaaa
     1021 cgccggcttg gcgcgttggg cgcgcaaccc cttccagagg acgaatccaa agaacgcggc
     1081 tgcacccgct cccagaccga tcgccagagg aagcgacagc cggccgaact gctccgctcc
     1141 aggctcgctg aacagaacca gcaggccggc gacgagagtg cctgccgcga ccgtggtcaa
     1201 caccccgtgc gtcggtgtgg tgaggtcata gataaagagc gcgaaagcga tcacgatcag
     1261 ggcgaggccg aaccagttca ccggaatcac gcccagtcca tacagcgcca acgccaggca
     1321 cacgacgcca atgaagcctg ctacccaacc gcccgggttc gacaactcga tcaggattgc
     1381 ctgtatgccg atgaacagca gaatcgccaa gatatttggg tcgatcaccc gattcaagag
     1441 ttgctcgaac caggacatct caagcgtttg aatttgcgct ccggtcgtgt tcagcatcac
     1501 cggacgattg gccacctcga cggtgaaccc gtccagttgc cgcagcaggt caggctcatc
     1561 gactgccacg aagtcgatca gagcggccgc cagcgcctcg tgcgcgtgga ccgccttggc
     1621 ctcttcgatc atcgcctccg cgagcgatgt agcctgctcg ccgcgtcgat cggtgagcgc
     1681 acgcgccagg gcgcgaatgt cttgcttgac tttctgctcg agcgtctcgc cgatatcttc
     1741 cccttcaccg ccgaccgggc tggctgcgcc gatcgtcgtc tccggcgcca tcgccgcgac
     1801 gtgagccgcg agtgtgatga gggtgccggc cgaggcggcc gccgcgccgc gcggcgagac
     1861 gtacaccacg agcggcacgt cgctggcgcg catgatctcc acgagctgct gcatcaggtt
     1921 aatctgaccg cccggcgtgt cgagcacgat taatagggct tctgcgccat ctcgctcggc
     1981 cgcggcgatg ctgcgctcga tgtgcgtggc ccacactggc gtgaggggac cctgcaacac
     2041 gatgcgatac acccgcggcg cttcatcctg cgccgctgcg gtcaccgtcg ccagtaacac
     2101 agtgaggaag ctgcatataa gcaacccgag catgcgccgt ttcatcgcca tcctcagtca
     2161 tatacattat atccccaatt gcacgcgacg ggtctcgcgg ccctgtacgc cctggcggaa
     2221 tgggagtaca atgcagtcag acccgtttta gatagcaagg aggaacacat gttagacgca
     2281 ctcttcagtt cgggcggcct ggtgctgctg tctctcatca tactgctgat cgtgatactc
     2341 acggcggcga tcaagattct tccagaatgg gagcgcggcg tgattctgcg cctgggccgc
     2401 ctgtcgagcg tgaagggacc gggactggtc gtgatcattc cgtttatcga ccggctcata
     2461 cgcgtcagca ttcgcgtggt gacgctggac gtgccgcagc agcgggtgat cacgctcgac
     2521 aacgtgacga tcgaagtgga cgccgtcgtg ttctaccgcg tcgtcgatcc ggcaaagtcc
     2581 gtcgtggccg tggaggactt ccggcgcgcc tctttcttcg tcacgcagac gacgctccgc
     2641 aacgtgatcg gtcaatcaga gatggacgag ctgctggcac gccgcgagcg catcaatgag
     2701 aaactgcagg tcatcatcga cgaggccacc gagccgtggg gcgtcaaggt cggcgccgtc
     2761 gaggtgagag acgtccgatt gcccgacacg atgcagcgtg cgatggccgc gcaggccgag
     2821 gcggaacgcg tgaagcgcgc caaggtcatt cacgccgagg gtgaatacca ggcggcgcag
     2881 cggctcgccg aagccgccgg ccgcatggcc gagcagccgg ccgccctcca gctgcgctac
     2941 ctgcaaacgc tgaccgagat cgcagccgag aaaaactcga cgatcatatt ccctctgcca
     3001 ctcgaatact tgaaggggtt tgaaaggctg ctcgccgaaa ggccgaaata gaccagccgc
     3061 catccgcgaa gcgtctcgca cctccgagcg ggatgcttcg ccactcactg aggcgctgag
     3121 aggttgaaga cgctgataat tatggcgcga cgctaggcag gcgccggcgt gtcgccggtg
     3181 gccggcgacg gatgttgcgg cctgccgccg gctatccgcc accacagccc ctcctcgatg
     3241 ggcgaccact ccctcggcag gggcacgcct gccagggcat ggtcgtcctg gatcgattgg
     3301 tggatctccg ccataacaac gcgaggcagc acccgcagcc cagcgacgat cagcagaggc
     3361 atatagatca gcacgagaca acacgcgatg aattcgccag gtccgagcac ggtgagcaag
     3421 ggaagcgcgc ctacggcgag cgcgttccag gcggcgtgga caattgccgc gcgtcccagg
     3481 ccgcgccaca gtgcgcgccg gtcacccgac agtcgcgcgc gtgcgtaacc cgcgccggtc
     3541 aacccggttg tgacgcagtg tgcgaccagg gtagccaggc gcacccacat caacacgagc
     3601 accgcaaaac tacccacacc ttcgaggata aagaacgcgc tctccagaaa gccgaacccc
     3661 aggccgatgg acatacccag caaaaggccg ccggtggtcg attggatctc ggcccgcacg
     3721 aaccgcaatc cgatcgcctt ggacgcttcc tcgatcggcg gcgctatgaa gacgaagctc
     3781 accagcagcc acgccagcag ctccggatcc acgatcacat tcggcaggcc ggattccgag
     3841 accaggtcta gaggtgactc tccgagagtc gcctgtccca gcaatccgcc aaacgtggcc
     3901 tccagaacaa gcgatgggaa ggtgctgaac attccccagg caaacgcgac aaacacggcc
     3961 cactcgtgga ctcgcaaccg tatggcctgc gatagctctg cgggaggcca tgcgcgctcg
     4021 accttgacgc caatccagcg ggagatccac actgcgcctg cgcccgctgc ggcgaatccg
     4081 atcgacgtgg ccaccagcat cgccggccca acgccgatca accggcccga gataaccgcg
     4141 ccgattccac cgccgaccag ccagacccaa acgaaggttg cgcgggccgg tcttggatcg
     4201 cgcggcgcgt acggccggtg cggcccggag gcggacctca gacggcgcgc ccgtgctgca
     4261 atcaagccgc cggcgacgat cgacgccgcg ccggcaacgg actgaagcct gagctgctca
     4321 tcgctcgacc cgagcggtgc gaccaaaagg acgaatccgg ccagcgcgct gatcacgccc
     4381 gccgcgattg tgagcgacag ccggcggcgc gcgcgaggcg accatccgcc cgggcttgtg
     4441 ctgggcggag ccgaagcatc ggacgccacg gcggactgca tcacgccttc ggcgttttca
     4501 gatggtgcga tggggttgtc caagcgctat ttcgtctttc gtctgcgctt cggcgcatcg
     4561 ggccgctcgc cgttcggttc gtggctcgac tggctgttgc ctttatcttc cgccggcttg
     4621 ccggctttct ccatccgttc ggcgatctcc ttgaggcgcg cctcgacgcg gccgtgcacc
     4681 gagtcaggag gaaatttccc tttcgcgccg cgcttgcccg ccttggcacc tgtcagcagc
     4741 tcgatacctt cgtcgaccgt gctgattgcc cagatgtgga agcgaccctc gtcgatcgcc
     4801 tcgacgacgt cctcgcgcag catgaggttc ttcacgttgg cggcaggtat catcacgccc
     4861 tgctcgccag tcagcccgcg cgcgtggcac acgtcgaaga acccttccac cttatgcgtc
     4921 actccgccga tcgcctgcac tcgaccgagc tgatcgaccg atcccgtcgc ggcgatgtcc
     4981 tgccggatag gcaggccgga taggctcgac aggagcgcgt acagttcggt gctcgacgcg
     5041 ctgtcgccgt cgacgtcgct gtatgactgc tcgaagacca aactcgcgct caacgtgagt
     5101 gggcgctcga cggcgtactg gctgcccaga tacccttgca ggatcaagac gcccttgtcg
     5161 tgaatgcggc cgctcaattt gacttcacgc tcgatattga tgacgccggc ccggcccagg
     5221 tacgtccgcg cgctgatgcg cgacggcagc gcgaacgcat gatcgccggc gatgcgcaca
     5281 gataatccat tgatctggcc gacgatcgcc ccctgtgtgg cgatcgtaat cgtgccgtcg
     5341 aggatcaagc ggcgcgactg ctgctcgatt tgatcggcgc gctggcgcga ttcgcgaatc
     5401 gcggtgcgca cgtcgtcggc tgtagcgaca tcgcgacccg ccttgctcgc ccagtacgcc
     5461 gcttcgcgaa ccacgttcgc cacttccccg aagcgcgtgc tgagtttctc ctgatcctcg
     5521 gccagccacg agccgtattc gacgatattt gccacggcgc cccggtcgag cgcgcacaag
     5581 ccctcttcgt cgcagcgtgc ccgaatgaac agcgcatagg cgtgctcgtt ttccggcgtg
     5641 cgatccatct cgggcgcgaa gtcggccttc accttgaaga gcttctggaa atcctcgtcg
     5701 accgcgtaga ggaggtagta caggctgggg ctgcccagca tgatgacctt cacgtcaagc
     5761 ggaatcggtt caggctcaag cgtgcgagtc gagaacatct gaaactgagt gcccggatcg
     5821 tcgatctgca cctggccact gtcgagcgca cgcttcaatg ccgcccaccc gatcggatcg
     5881 ttgagcacgt ccttggcgcg caggacaagg tagccgccgt tcgcgcggtg cagcgcgccc
     5941 gaccggagca tcgtgaagtc catcaccgtc ccgccgaagc gcacgtcgcg ctcgatgcgg
     6001 ccgatcaagt tcatgaacgt cgggttgcgc tcgttcacca ccggcgcgcc gagcgtgcgc
     6061 gcgtgatcga ccaggacatt cacctggtag cgcaggcgcg cggaggggcc gcgcagcgga
     6121 tcgggaggct gaggctgctg ctgttgactg gggctgaact ccccgatgtg gtcgatgata
     6181 tcccggcgca cttcctcgag atactcgacg acctctgggc agtcggtctg gtagcgctcg
     6241 atcagcgcat ccacgacgtg cccggcgacg ttcgccgcca cctggcggtc aagctcggcc
     6301 acggcctctt gagcctcctt ctcgtaaata cgcaccttgc gcaccgagtc ggccagaatc
     6361 tgctgcaggt ccatgcccgt ctgttccatc tgctgccgct gatcgggcgg caggccggcg
     6421 agcgcttcga tcggcgccgg cttcccgtcc acgagcggcg cgatcagcaa gccgccgggc
     6481 gtgcgcacga tggcgtaatt gcgctgcgcc gccgccgatt ccagctcgtg atacgccgcc
     6541 tgctgctttt cgtcgagctc gcgcgagatg cgggcgcgcg cctgactgta ttcctcggat
     6601 tcgaacgcgc gcggcacatc ggtttccagg tggccgacca gcgactgcat atcttgccga
     6661 aacgcgtttg cccgtccggg tgggagccgc atcgcgcgcg gcttgtaggg atcgacgaag
     6721 ttgtgcacgt acacccagtc gccagggacc ttctcgcgaa tagccttttc ggccagaaag
     6781 cgctcgatgg tggtagtgcg gccgctgccg cccggcccca atacgaatat gttgaagccc
     6841 ggcccgccga cgtcgatgcc gaagtcgatg gcgcgcgtag cgcgcggctg cccgaaaatg
     6901 tcggcggccg ccggcagctc agccgtcgtc ctgaaggtga atcgagatgg cgggcatgcc
     6961 cggcgggctt gcttcgcggt cagtttcttg attgccacgg catacctccc ctataggtgg
     7021 gggcgaccct tcggcttcgc tcagggcaag tgtcgaccaa cgacaacgcc cccagacggc
     7081 gtagggtccg ggggcgtcca gtcaagcggc aacctcgttc tatcgcccgc cgatgtgcat
     7141 cggcatgatc acgtgggtga aggcgtcgtc gccgatcggg cggatcatgc cgggcgacga
     7201 aggcgaagtc gtctccaacg ctatctgggg cgtgccggcg acgctcagga cgtcgatcat
     7261 gaacttcacg ttgaaggcga tctcgacctg cgcgccttcg accgaggcgt ccagctcgcc
     7321 ggcgttgtcg ccggtctcgg cgctggtcgc ctgcacggtc acgtgccccg gcgccagctc
     7381 gctcccgggg gcaatcgtca cccgtgcgat gttggaattc tcgcgggcga acacgcttgc
     7441 cgcccgacac gccttcagca gcggcgcggt atccatcacg gcgcgcgtct cataacgctt
     7501 aggcacgatc gcgctgtaat cggggaactt cccgtcgatc agttgcgaca ccagctcgac
     7561 gttgctcagg tgaaacagga cctggctgcg ctgcggcggg aacaggatac tgatcgggtc
     7621 tttttgctcg ccggatacgc ggcccagctc ggccagcgcg cgcgccggaa tgatcacttc
     7681 gatcggcttg ctcaccggcg tgagcagcgg cgctgcccgc accgacagcc ggaagccgtc
     7741 ggcggctgcg aagacgagct tgtcgccgct gaacttggcc agcacgccgg tcagaatcgg
     7801 gcggctttcg tccgtcgcgg cggcgaagac gacttgctcg atcatcttct tcagcgcgtc
     7861 aggcgcgatc tggatcgcgc tgtcttcttc gaagctcggg atgatcggaa actcctgcgc
     7921 gtcgatgcct ttgatgttgt tctcgaagcg tcccgagcgc acgttgagcg tttgggtgcg
     7981 caccgccaat tccatgtcaa cgcgatcctg cgggagcgcg ttgactaggt cgatgaaggt
     8041 gcgcgccggc accgtgatcg cgccgtcctc ctccactttc gcgccgatcc agcacgtgat
     8101 gccgatctcc agattcgtcg cggcgagccg taagcgaccg ttgtcggtcg cgagcagcac
     8161 gttgcccagc accggcagcg tcgagcgcgg cgcgacggcc cgtccgacga tgctcagccc
     8221 tttggccaga ttctcttgca agcaggatac tctcatggaa tgaagctccc ccggttaatt
     8281 ccgcgaatgc aaaataagta tagtccctgc caggcgaaat cgcaagaggg cgaaggacga
     8341 atttcgcaat gtgaagcggt cattcgtctt tcgtcattcg tctgtcgccc tgaagtcgta
     8401 cactccctcg cgcaccggcc aggcatgccc attggcgcac gtcagcgcgt cgggcgcttc
     8461 gcgccattcg gtcgatccgc actccgggca cttgaagagg ccaatcgctc gatcggtcgc
     8521 gcccgcgcgg cgcagcgtgc acagcaggaa ctggctgggc gccagcgcca atggagcgag
     8581 ggggcgctgc agtgcaccat cgatcgacgc cagcgtcttg gcgccgagca gtcgtttcaa
     8641 gaagccgacg cggaagaagg ataccgagcg gttctgccgg atattgaacc ccgccgcgcg
     8701 caattgagcc tgcatccagg ccgggtgaaa atcgaagttc agctcgacga actcgaccgg
     8761 ctcgcgggaa tagggcgacc agggctggcg gcggagagcg tagcgcagca tcgccttgag
     8821 gttacgcttg ttggcgaact cgacgacaaa cgtcccaccg gcgcggagcg ccgccgcgat
     8881 ttggcccagc gctttcggaa cgtcgaccag atgatgcagc acgcgcacca tcacgagcgt
     8941 gtcgaacgca gtgtcggcga acgggaggcg gtacacgtcc gcggcgacga atgtgaagcg
     9001 cgggtcgcgt ccccagcgcg cccgcgcttc gagcagcgtc gagcgcgcat agtccatcag
     9061 cacgatctcg tcgtaaccga gatacagctc cgccagccgt ccggcgcccg cgccgatctc
     9121 gacgagccgc cgcccgcgcg gcggcagcat cgcccgcagt gcgatccgct cggcgcgatc
     9181 ttcgtattca cgctgcggag tccagaactc tgtgctgtat ttcgagcctt cgtagtcgat
     9241 gacaggaggg gattgagtca tccatctacc gaggacgcat agaggctgag ttccggcatg
     9301 gggaagtgct tggcgttgag ggtgacgagc atcaggccgt ggcgaacggc tgtagcggca
     9361 atgatcgcat cggggatgct cgtgccatct ttgtggtgac ggcgcaagaa gtcgccagca
     9421 cgctcggcaa tctcacggtc cacatcatac gctgcaaagc gtgagagtaa cttacgagtg
     9481 tggtacgctt catgggggtg catccctgca tacacctcaa ggtgagtgac caccgataca
     9541 cccaagcggg tgcggcggcc aagttcacgt aagagatgca ccgcctcagg atcgccacga
     9601 aaatgtcgga tgacaaggcc cgagtcgagc agatgtccag ccatcgccta catccggcgc
     9661 gcctgtaatt gcgtgagacg ctcaggccat gtgccacgca agttagatag ccagcgatca
     9721 atatcttcag gtgtccgcat atcaggatga tcggtatctt tccaggcgcc cgcagtttct
     9781 tccagtactg ccgcctgttc tgctacgact aatctctctt caagcgcctg agcgacaaat
     9841 tcatcacgct tacgcgcggg aacttgcttc cgaagacgct gaatcaacgc tttaggtaga
     9901 gtcaccgtga ttttctgtgt tgtgttcgat gccctggtca tcctaccctc ccggcgagaa
     9961 ttatagcacg cactggcaca caaacctggt ttctctcaga aaccaggttt ccgccgctta
    10021 tggcccgacg aaggggcaca tgtccgccgc gacgtattgg cggttggcgt agccgaaggc
    10081 gttcaggccg tcgagcacat cggtctcgtt gttggcgtga gcgttggcgg cgaggcacgc
    10141 ctgactcagg cgatttgtct ccgcatactt cgaccagaag acctgcgcca attcttgata
    10201 ttcgtgaccg ggcgcgccgg ccgggaaatc cgcgctcagg cgatcgaacg cctcctgcgc
    10261 agaagccgca tcgcctgacc acgtatgggc tagcatgat
//
LOCUS       DBNHXB010000258         2902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2078020, whole genome shotgun sequence.
ACCESSION   DBNHXB010000258 DBNHXB010000000
VERSION     DBNHXB010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2078020"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="AC162G_10635"
     CDS             complement(<1..416)
                     /locus_tag="AC162G_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076318.1"
                     /translation="MSRRTRIVVTALAGIAILAGAVVVSNLPRRAPIPVTPSATPGPI
                     IQATSTPVPIPSSPPPRPVVGYTPIPDSVISPVVIQRSPETGQEQPPGSPIELVFDRP
                     MNRALVESAFQIYPAVAGTFNWVDDRIVQFQPGKELA"
     gene            complement(656..1558)
                     /locus_tag="AC162G_10640"
     CDS             complement(656..1558)
                     /locus_tag="AC162G_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR5076319.1"
                     /translation="MNTPTFGWVITPAARDASGAATLHENNRRFIERLRGAFDTIWVE
                     DHFQWNDRPVVECWTALTYLAAQYPDFTFGALVLGQSYRNPALTAKMMATLHWLTGGR
                     TIAGIGAGWKEDEYRAYNWPYPEPRTRIEQLEDAVNIIRAMWTQSPATYTGKHFSIKE
                     AYCEPRPNPPPPLLIAGGGEKLTLRVVAKYADWMNVGFRNAEAYARKLNALRGHCEQV
                     GRDYDSIKKTYFGFVSVTRDGARPEPRGDLHIVYGTPDQVAKELKELVELGVEHFMLR
                     FVDFPSTDGLELFLTEVAPVLTRA"
     gene            1796..>2902
                     /locus_tag="AC162G_10645"
     CDS             1796..>2902
                     /locus_tag="AC162G_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076320.1"
                     /translation="MSFAAKSAIPNDADAALRQPVLEQAAEGGMTLFTAPAGYLLTGS
                     LAAVLDGHGRSMLWLRLGPEDRDPAVLLMSLIASAQHIRPGLGTDTVSQMRRLPGPIQ
                     GWPHLFEHLGRELNEALPTSSAIVLEHVHYLNDVQPTLALLGHYILSALPSNVVCILA
                     SHNPLPPATLPVRTAYRGVNDLRLADHAALGLLESAEAKLPSALVYRANTLVGGRAGV
                     LADLFSAVALHGRASVQQAVERATSLEDLLTRVARAWLTTVNAETLQAIALATRLEFS
                     HPALIQAAIGVATTPAGPWLQPLADNWKRVRRVWLGPLRASSRAESTLSGDMLRRAAD
                     YLIGQDAIEPALRLYFEISDAARAAQVISGAADAM"
BASE COUNT          528 a          887 c          929 g          558 t
ORIGIN      
        1 gccagctctt tgccgggctg aaactgcacg attcgatcgt cgacccagtt aaaagtgccc
       61 gcgacggccg ggtaaatctg aaacgcagac tccaccaaag cacggttcat cggccggtcg
      121 aagaccagct cgatcgggct gccgggcggc tgttcctgcc cagtctcagg cgatcgttga
      181 atgacgacgg gcgatatgac gctatccggg atcggcgtgt agccgactac gggtcgcggg
      241 ggtggggaac tggggatcgg cacgggggtc gatgtggcct gaatgatagg gccaggcgta
      301 gcggaaggcg tgacggggat cggcgcgcga cgcggcaggt tggacaccac gacagcgccg
      361 gcgagaatgg ctatgccggc aagcgcggtg acgacgattc gagttcggcg ggacatggat
      421 cgttctcctc acgggcggaa ggcatagtgg ctctgaagaa atgacgcaaa atgcaggtgt
      481 cgggttcccg gaaatttttg gacgcggatc aacgcggatc acgcggattg tgattctagc
      541 tgcgctccca ccgggccggt ggcctggcga cccaagtcgg gagtcagtgc ggagcctccc
      601 cgcgggacag ccccccgacg accgacggga gcgtacaaga tgcatggcgc caggttcagg
      661 cgcgggtgag aacgggcgcc acctccgtca ggaacaactc taatccatcc gtgctcggaa
      721 agtcgacgaa gcgcagcatg aaatgctcga cgccgagttc cacaagttcc ttcagttcct
      781 tcgccacttg atccggcgtg ccgtacacga tgtgcaggtc gccgcgtggc tcgggcctgg
      841 cgccgtcccg agtcaccgac acaaagccga agtaagtttt cttgatcgaa tcgtagtcgc
      901 gccccacctg ctcgcagtgc ccccgcagcg cgttgagctt gcgggcgtat gcctcggcat
      961 tccgaaaccc gacgttcatc cagtccgcat acttcgcgac gacgcgcagt gtgagttttt
     1021 cgccgccgcc cgcgatgagc agaggcgggg gcgggttcgg gcgcggctcg cagtaagcct
     1081 ctttgatcga aaagtgcttg cccgtgtacg tggcgggcga ctgcgtccac atcgcgcgga
     1141 tgatgttgac ggcgtcctcg agctgctcga tgcgcgtgcg cggctccgga tacggccagt
     1201 tgtaggcgcg gtactcgtcc tccttccagc ccgcgccgat gccggcgatc gtccggccgc
     1261 cggtcagcca gtgcagcgtc gccatcatct tcgccgtgag cgccgggttg cgatacgact
     1321 ggcccagcac cagcgcgccg aacgtgaaat cgggatactg cgcggcgagg tacgtgagcg
     1381 ccgtccagca ctcgacgacg ggccgatcgt tccactggaa gtggtcttcg acccagatcg
     1441 tgtcgaacgc gccgcgcagg cgctcgatga agcggcggtt gttttcgtgc aaagtggctg
     1501 cgccggaagc gtcccgggcg gcaggtgtga tgacccagcc gaaggtcggt gtgttcatgg
     1561 cagctcctgt gcgtatagat tgtatgtcgc gccacgggag aagtcaaaca ataacgacaa
     1621 catgttgtcc atagccaaga ctccgctatc ggcttaaact atcccaacca atttcaaacg
     1681 gtggtgtgct acatttcacg ccttcagtga cagttgaaaa ggctgtgttt tgtcgtttat
     1741 catataacac agtcttcctc tttgtcctgg cacgctcgct gtgtgagaag tgaagatgtc
     1801 gttcgctgca aagtcggcta taccaaatga tgctgacgcg gcgctgcgcc agccggtgct
     1861 ggaacaagcg gccgagggtg gcatgactct attcaccgcc ccggccggct atctgcttac
     1921 cggcagcctg gcagcggtcc tcgacggtca tggccggtcc atgttgtggc tgcgtctggg
     1981 gcctgaggat cgtgatcccg cagttctcct tatgtcgctc atcgccagcg cacagcacat
     2041 ccgccccggg ctaggaacgg acacggtgtc gcaaatgcgg cggctgcctg gcccgattca
     2101 gggttggccc catctgttcg agcacctggg gcgagaactg aatgaagcac tccccacatc
     2161 gagtgccatc gtgttagaac acgtccatta cctcaacgat gtgcagccga cgctggcgtt
     2221 actgggccac tacattctgt cggcgctgcc atctaacgtc gtgtgtatac tggcgagcca
     2281 taatcctctg ccgccagcta ctctgccggt ccggaccgcc tatcgaggtg tgaatgatct
     2341 gcgtcttgcc gaccacgctg cgctgggtct gcttgaaagc gcggaagcga aattgccgag
     2401 tgctctggtc taccgcgcga acaccttagt cgggggccgg gctggtgtac ttgctgacct
     2461 cttctctgca gtggccctgc atggacgggc gtccgtccag caagccgtag aacgcgccac
     2521 cagtttggaa gacttgctga cccgcgtcgc gcgtgcctgg ttgacgacgg tcaatgctga
     2581 gacgcttcaa gcgatcgccc tggcgacgcg cctcgagttc agccatcctg cactgatcca
     2641 ggcggcaatc ggcgtggcga caacgccagc cggcccgtgg ctgcagccac tcgccgacaa
     2701 ttggaaacgg gtgcgccgcg tgtggctggg tccactgcgg gcgtcatcgc gagccgaatc
     2761 caccctgagc ggtgacatgc ttcgccgggc ggccgattac ctgatcggcc aggatgccat
     2821 cgaaccggcc ttgcgattgt acttcgagat aagcgacgct gcccgtgccg cgcaagtcat
     2881 ctccggcgcc gccgacgcaa tg
//
LOCUS       DBNHXB010000259         6340 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_65063, whole genome shotgun sequence.
ACCESSION   DBNHXB010000259 DBNHXB010000000
VERSION     DBNHXB010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_65063"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="AC162G_10650"
     CDS             <1..206
                     /locus_tag="AC162G_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010995232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR5076321.1"
                     /translation="QLGSPPSECVVVEDAAAGIEAAKAGGFRSVGLGPVERVGSADAV
                     FPNLTGVHLSDLLEALRGANPST"
     gene            264..737
                     /locus_tag="AC162G_10655"
     CDS             264..737
                     /locus_tag="AC162G_10655"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR5076322.1"
                     /translation="MHLRQPNARMCFICGLQNPVGLKMVIYNDLDNRQVVSTVTVPDH
                     FQGYPGVVHGGIVAAMLDEVAGRAVLLDGGDDDLMVTVRIEVKYRQPTPTLTPLTIVG
                     WVTHPGKTRARARGEVRLPDGTVTAEAELLLTLPPDDFRKRWAEEKPYWKVYADA"
     gene            747..2690
                     /locus_tag="AC162G_10660"
     CDS             747..2690
                     /locus_tag="AC162G_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076323.1"
                     /translation="MSFLHRLRPRTLDALWLAGLLAAALLVYGTSLTYPFFWVDPIDI
                     GLAASRDIPTILTNSQGYLYYRPFAFMLWKALYTLSGRYDPFAFHLVHVVCHTISAWL
                     FYALARRILGDRTPAGIAGLLFVWYPASYQAVTWVISPQVQAMPFLLGSAIAYTDGRT
                     RGDRRRIILSLLLLAIALPFHENAASFGFVIAALEGFFVAGRRIGEQEGRRVSPWPLA
                     HLAICAAFAALWFAVPKDPDSAVARFEPAAGWYLLQGAIWPVAGAVGWWRDGLDGPAW
                     QPLILTAPMTLLFLLAAYAYARRLPLFGLGLSWFAAMTLPIWATRGISYVGISPRVFY
                     IAAPGAILTWAGLLSLDLRSPNVNRVWKMGLGLLTALIVVQSAAFLGVRKDVHDQATT
                     AIWDVVRIGQSAGDEASLLFVNAPDQITPRLREYPVGFFRAILMPVSVDLGQYTELQT
                     GVRPATTSLSVPWLAGLEHYPHQVDMRGPPVGQAELSAAIRAADGVYLTEYEPGGRVR
                     VVEAGSVTPAAAEPYQATFGGRAGLVNATLTPEGDRRRVALIWECLARFDRQDTIFLH
                     AVDGDRLVAQSDGDPLRGLFPLSECRPGERIRDVRFIEAPPGADVIRVGIYNRLTGER
                     LIAIDANGQSLPDHAVVIGQVTR"
     gene            2687..4792
                     /locus_tag="AC162G_10665"
     CDS             2687..4792
                     /locus_tag="AC162G_10665"
                     /inference="COORDINATES: protein motif:HMM:NF021134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HIR5076324.1"
                     /translation="MTRLKPHVSRFTHHVSRITHHDLTLIVAVALVARLMAIVALRVG
                     GYVAETGPDSAYHFQLGRLAASGAYPFIDYWTEYPPVFPWLAVLAHKISTLMPSWIDQ
                     RFWFNLSLHGLIAPFEAGNVVFVYALSRKAGGAPDALKGAWLYALLFAPMFVVLGWFE
                     SIALFAVLLALWLILEDRPIPAGVAIGLGMLVKPYVALIGAVGLIHLSTWRKAGLLVA
                     SAALAYLAGLSPFLIASPEMVMAHFTTLLALPGWSSPYALIDGVIKHADPQLADRFDA
                     ALAASPIAPSQVPWAAVTLAFAAVYAFVLFRAYGQQHNRAAVGLAGFSFVMYLLWSKG
                     YSPQWSLYLIAFLCILMPNLRGMLMLITLELLYVIEWPITFILLKADPAYLTALVAVR
                     TLVTIGLGVLFAALIFVKSDQAWSRLRKGSIAGSLAALGAVTLLAAGALPVYHAQRYR
                     IEPMREAVELIQSSSAPDVAGVLFDRVDTYERLASFLSRWPKLTALRLGGAADVWSEA
                     EILSFATGRPELWYVLDYGAAAHRVIGDDIDRYLSDSLCIVSRQFAGAARVARYANAK
                     PETGLGVSADFENGITLAGARINNTTIGQDDPICVELVWTADDTPSTDYTVFVHLIDA
                     AGNLVAQSDIQPGSGYTPTTTWTPDQPMLDRHGLIPPAGLAPGIYRLTVGMYDLEGER
                     LHTVDGADSAVLGEIEVGD"
     gene            4854..>6340
                     /locus_tag="AC162G_10670"
     CDS             4854..>6340
                     /locus_tag="AC162G_10670"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5076325.1"
                     /translation="MTPEEYLEALLKLPGMWTPEVSPDGKWVAWMWFRTGPAGDVYAA
                     PTEPALSGGEGSPPIRLTETPDDTVVVSWTPDSQAVIVEQDKDGNERAQLFRVDLARP
                     GVMVPLTEPDPHYFIRGGELHPNGRWLVYGANFDFEADREIEPTWVYRHDLETGKRRV
                     LARTQKGCWYRPELNTPGTHILYSRNERHPSGRQVWLVDIEGSEDREVLNFGADAKVF
                     ASWFPDGRRALVVAETKTHRRVGVWDLKHETTRWLIDDPDRNIESAHVPHGSDRAVVV
                     EIREARPRASLLDVQTGEEFRLPEIGGSLMPLAPAGDAAWVGQYYSSRQPADLVCFSP
                     LDPNPARFTSVSRVWERTSLTPDDLTPAEDFRWQSVDGLEIQGWLYRPQGKARGTIVF
                     VHGGPTFHSADAINNQIQFFARQGFNVLDPNYRGSTGFSLAFREAIKVDGWGGREQGD
                     IRAGIEALIAAGIAEKGKVGVTGTSYGGYSSWCAITRLPKDVTAAS"
BASE COUNT         1033 a         2048 c         2069 g         1190 t
ORIGIN      
        1 cgcaacttgg ctcgcccccg tctgagtgcg tggtggtcga agatgccgcc gccggcatcg
       61 aggcggcgaa ggcgggtggc tttcgctcgg tcggtctcgg tccggtggag cgcgtgggca
      121 gcgcagacgc cgtctttccc aatctcaccg gtgttcacct gtcagacttg ctcgaagcgc
      181 tgaggggagc gaacccatcc acgtaatatc gaagatgggc cgcgcatggc ctatatatcc
      241 tgcaagatag gagagcgacc gctttgcatt tacgacaacc gaacgcgcgc atgtgcttca
      301 tctgcggcct tcagaatccg gtcggcctca agatggtgat ctacaacgac ctcgacaacc
      361 ggcaggtcgt gtcaaccgta accgttcccg atcactttca aggctatccc ggcgtggtgc
      421 acggcgggat cgtcgccgcg atgctggacg aagtggccgg gcgcgccgtg ctgctcgacg
      481 gcggcgacga cgacctgatg gtcacggtca ggatcgaggt gaagtatcgg cagccgactc
      541 cgacgctgac acctctgacc atcgtcggct gggtcacgca tcccggcaag acgcgggcgc
      601 gggcacgcgg cgaagtccgc ctgcccgatg gcacggtcac tgccgaggcc gagctgctgt
      661 tgacactccc ccccgacgac ttccgcaaac gctgggccga agagaagcca tactggaagg
      721 tttacgccga tgcctgacgt ctggctatga gcttcctcca tcgcctccgg ccacgcaccc
      781 tcgacgcgct gtggctcgcc gggctgctcg ccgctgcgct tctggtctac ggcacatctc
      841 tcacataccc attcttctgg gtcgatccga tagatatcgg cctggctgcc tcgcgcgaca
      901 tccctaccat cctcacgaac tcgcagggct atctttacta ccggccattc gctttcatgc
      961 tgtggaaagc actatacacg ctgtccggcc gctacgaccc gttcgctttc cacctcgttc
     1021 acgtcgtctg ccacacgatc agcgcctggc tgttttacgc gctggcgaga cgcattctcg
     1081 gcgaccgcac gcccgccggc attgcgggac tgctgttcgt atggtatccg gcgagttacc
     1141 aggcggtgac gtgggtcatt tcgcctcagg tgcaggccat gccttttctg ctcggctcgg
     1201 ctatcgccta caccgacggg cgaacgcggg gagatcgtcg gcgaatcatc ctctcgctgc
     1261 tgctcctcgc catcgccctg cccttccatg agaatgccgc ttcgttcgga ttcgtgatcg
     1321 ccgcgcttga gggctttttc gtcgccggcc gccggattgg cgaacaggag ggcaggcggg
     1381 taagcccctg gccgctcgcc cacctcgcga tctgcgccgc gttcgctgcc ctgtggtttg
     1441 ccgtccccaa agaccccgat tcggcggtag ctcgcttcga gccggcagcc ggatggtatc
     1501 tgttgcaggg cgcgatctgg ccggtggcgg gcgcagtcgg ctggtggcgc gacgggcttg
     1561 acgggccggc ctggcagccg ctgatcctca ctgcgccgat gacattgctg ttcctgctcg
     1621 ccgcgtacgc ctatgcgcgc cggctaccgc tgttcgggct cggcctgtca tggtttgctg
     1681 ccatgaccct gcccatctgg gcgacgcggg gcatcagcta cgtgggcatc tcgccgcgcg
     1741 tcttctacat cgccgcgccg ggcgcgatcc tgacgtgggc cggcctgctc tcgctcgatc
     1801 ttcgatctcc taacgtgaat cgcgtctgga agatgggcct cggcctgctc accgcgctga
     1861 tcgtcgtgca aagcgccgcc tttctcggcg tgcgaaagga tgtgcacgat caggcgacca
     1921 cagcgatctg ggacgtcgtg cgcatcggcc agtcggcagg ggacgaagcc agcctgctct
     1981 tcgtcaatgc gcccgatcag atcacgccgc gcctgcgcga atatccggtc gggttctttc
     2041 gtgccatcct gatgccggtg tcggtcgatc tgggccagta caccgagctg caaaccggcg
     2101 ttcgaccggc aacgacttcg ctgtccgtgc cttggctggc cggattggag cactacccgc
     2161 atcaggtcga catgcgcgga ccgccggtcg gtcaagcgga gctgagcgcc gccatccgcg
     2221 ccgccgacgg cgtgtacttg accgagtacg aacccggtgg acgcgtgcgc gtagtcgaag
     2281 cgggcagcgt gacacctgca gccgccgagc cgtatcaagc gaccttcggc ggacgcgccg
     2341 gcctggtcaa tgccacactc acaccggagg gcgatcgccg gcgtgtggcg ctgatctggg
     2401 agtgcctcgc gcgattcgac aggcaggaca cgatattcct gcacgccgtc gatggcgatc
     2461 gactcgtcgc gcagtccgac ggcgatccac tgcgcggcct ctttccgttg tccgagtgcc
     2521 ggcccggtga gcgcatccgc gacgtgcggt tcatcgaagc gccgcccggc gcagacgtta
     2581 tccgcgtggg gatatataat cggctcaccg gcgagcgcct cattgccatc gacgcaaacg
     2641 ggcaaagtct gcccgatcat gccgtggtca tcggacaagt gacaaggtga cgcgcctcaa
     2701 acctcacgtt tcacgtttca ctcatcacgt atcacgcatc acgcatcacg atctgaccct
     2761 gatcgtcgcc gtggcactcg tggcgcgcct gatggcaatc gtcgcgctgc gcgtgggcgg
     2821 ctacgtcgcg gagaccggcc cagacagcgc ttatcacttc cagttggggc gcttggccgc
     2881 cagcggcgcc tatccgttca tcgactactg gacggagtac cctcctgtct tcccgtggct
     2941 ggccgtgctg gcgcacaaga tttcgacgct catgccttcg tggatcgacc agcgcttctg
     3001 gttcaatctc agcctgcacg gcctgattgc gcccttcgag gcagggaatg tcgtcttcgt
     3061 ctacgccctt tcgcggaaag ccggcggtgc gccagacgcg ctcaaaggcg cgtggctgta
     3121 cgcgctcctg ttcgcgccga tgttcgtcgt gctgggctgg ttcgaatcga tcgctctgtt
     3181 cgccgtgctg ctcgcactgt ggctgattct cgaggatcga ccgatccccg ctggcgtcgc
     3241 catcgggctg ggcatgctcg tcaagcccta cgtcgcgctg atcggagccg taggcttaat
     3301 acacctgtcc acgtggcgca aggccggcct tctcgtggcg tctgcggcgc ttgcgtatct
     3361 ggcgggcttg agtcccttct tgatcgccag cccggagatg gtgatggccc atttcacaac
     3421 gctgctggcg ctgcccggct ggtcgtcacc gtatgcgctg atcgacggcg tgatcaagca
     3481 tgcggatccg caactggccg accgtttcga tgccgctttg gccgccagcc cgatcgcgcc
     3541 gtcgcaggtg ccgtgggccg ccgtcacact tgccttcgcc gccgtatacg cctttgtgct
     3601 gtttcgcgcg tacggccagc agcataaccg cgccgccgtc gggctggccg gattcagctt
     3661 cgttatgtac ctgctgtggt cgaagggcta cagcccccag tggagcctgt atctgatcgc
     3721 gttcctatgc atcttgatgc cgaacttgcg cggcatgctg atgctgatca cgctcgagct
     3781 gctgtacgtc atcgaatggc cgatcacgtt catcctgctc aaggccgacc cggcctacct
     3841 gacagcgctg gtcgcggtgc gcacgctggt gacgatcggg ctgggcgtgt tgttcgccgc
     3901 gctgatattc gtcaagagcg accaggcgtg gagccggctg cgcaaaggca gcatcgccgg
     3961 cagcctggcc gcgctcgggg cggtcaccct gctggcggcc ggcgcgctac cggtgtacca
     4021 tgcgcaacgc tatcggatcg agccgatgcg cgaggccgtc gagttgattc agtcgtcctc
     4081 ggcgcccgat gtggccggcg tgttattcga ccgcgtcgac acctacgagc ggctggcgtc
     4141 gttcctgtcc cgctggccga agctcaccgc gctgcgcctc ggcggcgcgg ccgacgtctg
     4201 gagtgaagcg gaaatcctgt catttgccac cgggcgacca gagctgtggt acgtgctcga
     4261 ctacggcgcc gcagcgcacc gggtcatagg ggacgacatc gatcgttacc tgagcgactc
     4321 tctgtgcatc gtcagccggc aattcgcggg ggcggcgcgt gtcgcgcgct acgcgaacgc
     4381 gaagccggaa accggcctgg gcgtctcggc cgatttcgaa aatgggatca cactggcagg
     4441 cgcgcgcatc aacaacacga ccatcggaca ggacgacccc atctgtgtcg agctggtatg
     4501 gacggcggac gatacgccgt cgactgacta caccgtcttc gtccacctga tcgacgcagc
     4561 cgggaatctc gtcgctcagt cagacatcca gcctggcagc gggtacacgc cgacgacgac
     4621 ttggacgcct gaccagccca tgctcgatcg gcacgggctg atcccgccag cgggccttgc
     4681 gccgggcatc tatcggttga ccgtgggaat gtacgacctg gaaggagagc gtttgcatac
     4741 agtagatggc gccgattcgg cggtgctggg ggagatagag gttggagatt agagattgga
     4801 gattggagat tggagattgg aggttggagg ttggagattg gaggttggag aagatgacgc
     4861 ctgaagagta tctggaagcg ctattgaagc tgccgggcat gtggacaccg gaggtctcgc
     4921 cggacggcaa gtgggtcgcc tggatgtggt tccgaaccgg cccggcaggc gatgtctacg
     4981 ccgcgccgac agagcctgcc ctgagcggag gcgaagggtc gccgcccatc cgcctgaccg
     5041 agacacccga cgatacggtc gtcgtgtcgt ggacgccgga cagccaggcg gtgatcgtcg
     5101 agcaggacaa ggatggcaac gagcgcgcgc agttgttccg cgtcgacctg gcccgccctg
     5161 gcgtgatggt cccactcacc gagcctgacc cacattactt catccgaggc ggggagctgc
     5221 atcccaacgg gcgatggcta gtgtacggcg cgaacttcga cttcgaagcc gatcgagaga
     5281 tcgaaccgac gtgggtgtat cggcacgact tggagaccgg caagcggcgc gtattggcgc
     5341 gcacgcaaaa gggatgctgg tatcggcccg agctgaacac tccggggacg cacatcttgt
     5401 attctcgcaa cgagcggcac ccgagcgggc gacaggtgtg gctggtggac atcgaaggga
     5461 gcgaagatcg ggaagtcctc aacttcggcg cggacgcgaa ggtcttcgcc tcgtggtttc
     5521 ccgacggccg gcgcgcgctg gttgtcgccg agacgaagac gcacaggcgc gtcggcgtgt
     5581 gggacctgaa gcacgaaacg acgcgctggc tgatcgacga tcccgatcgc aacatcgaaa
     5641 gtgcacacgt cccgcacggg agcgatcggg cagtggtcgt ggaaatccgg gaggcgcggc
     5701 cgcgcgcatc cctgctggac gtgcagaccg gcgaagagtt ccggctgccg gaaatcggcg
     5761 gcagcctgat gccgctcgcg ccggcgggcg acgcggcgtg ggttgggcaa tactacagct
     5821 cgcgccagcc ggcagatctg gtgtgcttct cgccattaga tccgaacccg gctcgcttca
     5881 ccagcgtgag ccgagtgtgg gagcgcacct cactcacgcc cgacgacctg acgccggcag
     5941 aggacttccg atggcagtcc gtcgacgggt tggagattca aggctggttg tatcggccgc
     6001 agggaaaggc gcggggcacg atcgtgttcg tccacggcgg accgactttt cacagcgccg
     6061 acgcgatcaa caaccagatt cagttcttcg cgcgacaggg cttcaacgtg ctcgatccga
     6121 actatcgcgg cagcaccggc ttcagcctcg ctttccgcga agcgatcaag gtcgacggct
     6181 ggggcgggcg cgagcagggc gatatacggg ccggcatcga agcgttgatc gcagccggca
     6241 tcgccgagaa gggcaaggtc ggcgtgaccg gcacgtcgta cggcggctat tcgtcctggt
     6301 gcgccatcac ccgcctcccg aaggacgtca ccgccgcgtc
//
LOCUS       DBNHXB010000260         4333 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1407547, whole genome shotgun sequence.
ACCESSION   DBNHXB010000260 DBNHXB010000000
VERSION     DBNHXB010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1407547"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="AC162G_10675"
     CDS             complement(<1..473)
                     /locus_tag="AC162G_10675"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(342..344),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR5076326.1"
                     /translation="MSKAALNAQAPDFTLDDFEGHPVRLSDYRGRKNVVLVFNRGFFU
                     PFCRRHMAQLRRDYHRFAERNAEVIVVGPDDASAFADYWREESLPFIGLPDPGGSVLK
                     RYGQEFNLFKLGRMPAQAIVDKAGRVRYLHYGRSMSDIPSGEQLLDILDELNRQD"
     gene            complement(479..1435)
                     /locus_tag="AC162G_10680"
     CDS             complement(479..1435)
                     /locus_tag="AC162G_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5076327.1"
                     /translation="MTRKESQLESIALGATDVRVPPIGIGTWQWGDTLVWGYGRGGYT
                     DADLRAAFDACLVNGIDFFDTAEGYGRGRSERLLGEFIRASGRPAIVATKFMPLPWRL
                     RHGSLVAALRRSLGRLGLARVDLYQIHWPFPPRSVETWAGALADAVEAGLARAVGVSN
                     FSAGQMRRAHQALARRGIPLASNQVGYSLLQRGPERSGLLAACRELNVTLIAYSPLAK
                     GLLTGKYSPEHRPSGVRRWMWSREKLAHAQSLVALLRHIGESHGGKTLSQVALNWLMC
                     KGALPIPGVKNARHVSDNLGAVGWRLTDEEVAALDAASERVV"
     gene            complement(1439..2383)
                     /locus_tag="AC162G_10685"
     CDS             complement(1439..2383)
                     /locus_tag="AC162G_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIR5076328.1"
                     /translation="MRVVITGGTGLIGRALASSLAADRHDVIILSRNPKYARSLPKGV
                     RAEQWDGRTASGWGHLADGADAIVNLAGASIAGDGLVGVFLQRWTSRRKRLILESRLN
                     AGRAVLQAIEAAKKKPGVLVQASAIGYYGAAREEALTEDDTRGFDFQARVVWDWEVST
                     SQAEVMGVRRVVTRSGLVMSRNGGMLPVIALPFKFFLASRLGSGRQWFSWVHVADEVG
                     AIRFLIENPTARGPFNLTAPDAITNAQLTRLLRRILRRPPFIPAPAFALRLLLGEKST
                     LILAGPRPVPKRLTEMGYAFKFPQAEAAFRDLLSKGKR"
     gene            2690..3226
                     /locus_tag="AC162G_10690"
     CDS             2690..3226
                     /locus_tag="AC162G_10690"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5076329.1"
                     /translation="MSQRSYPERVTLRDGTTVTLRPIRPDDAPRLQEAFERLSPESVF
                     LRFLDQRALLSDVEARRFATVDGHSTMAIVAVIEEHGEEHIIGSARYAVVGPAKPDTA
                     EAAVTVIDEYQRRGLGTILVQHIVEYALAHGIRYFLATVHYSNEQILSFIERSKLRAE
                     KRLESGAWEIKILLDAQA"
     gene            3571..4017
                     /locus_tag="AC162G_10695"
     CDS             3571..4017
                     /locus_tag="AC162G_10695"
                     /inference="COORDINATES: protein motif:HMM:NF015085.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HIR5076330.1"
                     /translation="MQPKFLLGGALLTMAVILVIAGGFAGGAQYFLTVDELRAKGAEF
                     QDQNVRISGAVIGDSVRTYVNSQTPIVEFDVVDAVEMDGVFQPVVGQTPLHIVYEGPR
                     PELLQDDAQAIMEGRLSADGAFYADTLLLKCPTRYEEQFPEQAQGN"
     gene            4084..>4333
                     /locus_tag="AC162G_10700"
     CDS             4084..>4333
                     /locus_tag="AC162G_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076331.1"
                     /translation="MIVDIGLIALVLAFASAAYAVGAAAYGGATKRPTWVASARYAAL
                     TIAPLLTIACLMVILSNLTDNYNLAYTYRVSNRAQLTVL"
BASE COUNT          808 a         1440 c         1320 g          765 t
ORIGIN      
        1 tcatcctgcc ggttcaactc gtccagaatg tccagcagtt gttcgccgga gggaatgtcg
       61 ctcatcgacc tgccgtagtg caggtagcgc actcgaccgg ccttgtcgac gatagcctgc
      121 gcgggcatgc ggccgagctt gaacaggttg aattcctggc cgtagcgctt cagcacgctg
      181 ccgcccgggt caggcaggcc gatgaaaggc aaactctcct cgcgccagta gtcggcgaag
      241 gcgctcgcgt catccggccc gacgacgatc acttcggcgt tccgctcagc gaagcgatga
      301 taatcgcggc gcaactgcgc catgtgccgg cggcagaacg gtcaaaagaa accacggttg
      361 aacaccaaca cgacattctt acgaccccga taatccgaca gccggaccgg atggccctcg
      421 aagtcgtcga gggtgaagtc cggcgcctgt gcgttgagcg ctgctttcga catggcttct
      481 acacgacccg ttcactcgca gcgtcgagcg cggcaacttc ctcatccgtc aaccgccaac
      541 cgaccgcacc caagttgtct gatacgtggc gcgcgttctt gacgccgggg ataggcagcg
      601 cgcctttgca catcagccag ttgagcgcaa cctgtgacag cgtcttgccg ccgtggctct
      661 cgccgatatg ccggagcagc gcgacgagcg actgggcatg ggccagcttc tcccggctcc
      721 acatccagcg gcgcacgccg ctcggacggt gctcgggcga gtacttgccg gtgagcaggc
      781 ccttggcgag cgggctgtag gcgatgagtg tgacgttgag ttcgcggcag gcagccagca
      841 gcccgcttcg ctccggcccg cgctgcagca ggctatatcc gacctggtta gaggcgagcg
      901 ggatgccgcg cctggccagc gcctggtgcg cgcgccgcat ctggcccgcg ctgaagttcg
      961 agacgcccac cgcgcgcgcc agcccggcct cgactgcgtc agccagcgca cccgcccacg
     1021 tctcgaccga gcgcggtggg aacggccagt ggatctggta aagatcgaca cgcgccagcc
     1081 ccagccggcc aagacttcgt ctcagcgcgg caaccaggct gccgtgtcga agccgccacg
     1141 ggagcggcat gaatttcgtc gctacgatgg ccggccggcc ggaagcgcga atgaattcgc
     1201 cgagcagacg ttcggatcgc ccacggccat acccttctgc cgtatcgaag aagtcgatgc
     1261 cgttcaccag gcacgcgtcg aacgccgcgc gcaggtcggc gtcggtgtaa ccgccgcggc
     1321 cataacccca caccagcgtg tcgccccact gccacgtgcc gatgccgatc ggcggcacac
     1381 gcacgtccgt cgcgcccagg gcaatggact cgagctgaga ttcctttcga gtcacggatt
     1441 acctctttcc tttcgacagc aaatccctga acgcggcttc cgcttgcggg aacttgaaag
     1501 catagcccat ctccgtcagg cgcttcggca ccgggcgcgg tcccgccagg atgagcgtcg
     1561 acttctcgcc gagcagaagg cgcagcgcaa aagccggcgc cgggatgaac ggcggccgcc
     1621 gcaggatgcg tcgcagcagg cgggtcagtt gcgcattcgt gatcgcatcc ggcgccgtga
     1681 ggttgaacgg cccgcgcgcc gtcggatttt cgatgaggaa gcgaatcgcg ccgacctcgt
     1741 cggcgacgtg cacccacgag aaccattgcc ggccgctgcc cagccgcgaa gccaggaaga
     1801 acttgaaggg gagcgcgatg acgggcaaca tgccgccatt ccggctcatc accaggccgg
     1861 aacgagtcac cacgcgccgc acgcccatca cctcggcctg cgaagtagac acctcccaat
     1921 cccacaccac gcgcgcctga aaatcgaagc cgcgtgtatc gtcctcggtc agcgcttcct
     1981 cgcgcgcagc gccgtagtaa ccgatggcag acgcctgcac cagaacgcct ggcttctttt
     2041 tcgctgcctc gatcgcctgc agaacggcgc gccccgcatt caaacggctc tccaggatca
     2101 ggcgcttgcg ccgagatgtc cagcgctgca agaaaacgcc cacgagaccg tcgcccgcga
     2161 tgctcgcgcc ggccaggttg acgattgcgt ccgcgccgtc ggccaggtga ccccagccgc
     2221 tcgccgtgcg cccatcccat tgttcggcac gcacaccctt gggaagcgat cgagcgtact
     2281 tcggattgcg gcttagaatg atgacgtcgt gcctgtccgc tgcgaggctg gatgccagtg
     2341 ccctgccgat gagtccggtt cctccggtga taacgacgcg catacgttgg tctcctcgaa
     2401 ggcttccgaa gtcttgcaga cttcggaagt ctgaacttga ttcagccagt cgaggatagg
     2461 cgcaccatgc ctatccaatc cctcgggtct ggcattaacg gcccatgatc cgacacaagc
     2521 gaggatgccc taattatcgc acgtgttgag cctttgtcca cattgcgcct ggcataggtc
     2581 gtcaatcctg cgacgaacac ccagccgatc gcccgactca ggtataatgt gaccagtgtc
     2641 tgcaccgttc ccagcgaagg tcggctgttg ttccggggag caagcaccta tgagccagag
     2701 gtcatatcca gaaagagtca cgctgcgcga tggtacgacc gtgacgcttc gcccgattag
     2761 gcccgacgat gcgccccggc tccaggaggc gttcgagcgg ctttcgccgg agagcgtctt
     2821 cctgcgcttt ctcgaccagc gcgccttgct gtctgacgtc gaagcgcggc gcttcgcaac
     2881 cgtcgacggc cacagtacga tggcgatcgt cgcggtcatc gaagagcatg gcgaggagca
     2941 catcatcggc agcgcgcgat acgcggtcgt cggtcccgcg aagccggaca cggcagaggc
     3001 agcggtgacg gtcatcgacg agtatcagcg gcgcgggtta ggaaccatcc tcgtgcagca
     3061 catcgtcgag tatgccctcg cgcacgggat tcgctatttc ctggcgaccg tgcactacag
     3121 caacgagcag attctgtcct tcatcgagcg aagcaaactc cgggctgaga aaagactgga
     3181 gagcggcgcc tgggagatca aaattctgct cgacgcccag gcgtgattcg cgccgtcgtc
     3241 tttccgctgg cagtcgtcgc gcccgtttct ggctcgctgg attccctgcg tgcatttcag
     3301 gttggtggcg gatattgcct cacccccctc ccccctctcc tgacgacagc cgtttcgtcg
     3361 tcaggagagg ggccgggggt gaggtcaagc agccagcccg ccgattcgcc ccagaattga
     3421 aatgcgcccc attccccggt gaggttgatc cgctgacaaa tgtcaattaa aatagatgat
     3481 acaccgcatt gacactaccc gttcgggtag ggtataactt catctgtgaa tacccacccg
     3541 gtcacataac gaaacctgga ggagagtctg atgcaaccca aattcctttt gggcggtgcg
     3601 ctgctcacca tggccgtcat tctggtcatt gctggaggct ttgccggcgg cgcccagtac
     3661 ttcctgaccg tcgacgagct gcgcgcgaag ggagcggagt ttcaggatca gaacgtgcgc
     3721 atctccggcg cggtcatcgg cgacagcgtc cgcacgtacg tcaattcgca gacgcccatc
     3781 gtggagtttg acgtcgtcga tgccgtcgag atggacggcg tattccaacc ggtcgtcggg
     3841 cagacgccgc tgcacatcgt gtacgaaggg ccgcggcccg agctgctcca ggatgatgcg
     3901 caggcgatca tggaaggccg cctgagcgcg gacggcgcgt tctacgccga cacgctcctg
     3961 ctcaagtgcc ccacccgcta cgaagagcag ttccccgaac aggcgcaggg aaattgaccg
     4021 tcgccagact tccgaagtct ttggagactt cggaagtctt gagccaaggg agagaggtgt
     4081 ctcgtgatcg ttgacatcgg attgatcgcg ctcgtgctcg cgttcgcctc ggcggcctac
     4141 gccgtcggcg cggcggccta cggcggcgca acgaaacgac ccacctgggt ggccagcgcg
     4201 cgctacgcgg cgctcaccat cgcgccgctg ttgaccatcg cctgcctgat ggtcatattg
     4261 tctaacctca ccgacaacta caacctggca tacacctatc gcgtcagcaa ccgcgcccag
     4321 ctcacggtcc tga
//
LOCUS       DBNHXB010000261         5296 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4595131, whole genome shotgun sequence.
ACCESSION   DBNHXB010000261 DBNHXB010000000
VERSION     DBNHXB010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4595131"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2415
                     /locus_tag="AC162G_10705"
     CDS             <1..2415
                     /locus_tag="AC162G_10705"
                     /inference="COORDINATES: protein motif:HMM:NF014877.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR5076332.1"
                     /translation="ENAWTQSTSAKVFSLLLTLMAEAGQYRHETYRPPLDADQTRYRM
                     LQDQVLPGMGRLRAVAVKLAKKYLSRPVFASVARDIEEEIFPLLDDMDPAVAPDRYMP
                     FRVIHVGNVVERLYGFRFRTGDPTLVGDSNGAGLLQEIYERKYLRFGTSGVRGRWGRD
                     FTEERAKQVVQAICDFLKNQDAPPYAAAENLQGRRMVIGYDSRLNARLVASWAAQVCL
                     ADGFSVDQANRDTPTPALVYYMTDYLGADEVAGLINCTASHNPPEWQGIKFNPRLGYP
                     APTNLTDFIASRINEIQIVGRRNTEAALEDQTEQGRLRGFDPIDLYTNWILDSGRGNP
                     RIAPDFDRIRKYFGDQVVVVDEMHGAGRGYLTRLLGEIGVRCTVIHAETDPMLPGLDY
                     ANPEEPFINSLKEKVKESGAILGLGLDTDADRFGIVDRGGIYLRPNQILPILVRYLGV
                     DRGLNGRVIATQTGSPLIEPLAGMIRGNEAFMPESGVIPAYIDHPFYQRRVGQREDRV
                     YKNTFMVPVGIKYIEEQRRTDRRYRSLPQLLDNWRSVLLIGGEESSGLTTRGHVTDKD
                     GIWANLLVMDMLAYYGTLPDKSLSSLSDIWQDTCQLPGCWLSYGGRELRGSNAGRADV
                     DAILEAKEDLISYYLDLFSATKPDNKPALSGAEGLADLNVVYAGGVRYDICELQLRDA
                     QGDDRHFLRVRSSGTEPINRIYVESSNPEIAQRLMRTVLDKLEELIAREIGQAQTEWR
                     LVDVLTLTQMSPRLLDTVQKALAERGWSVASVTGKLKRIIHAPNYLEHRNRRTALGWV
                     NALESP"
     gene            2470..3300
                     /locus_tag="AC162G_10710"
     CDS             2470..3300
                     /locus_tag="AC162G_10710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR5076333.1"
                     /translation="MIRLVDTHCHLDWHTFDADREEAIWRAVDAGVTRMVTVGVSVAS
                     SRMAVELAGRYEPVYAAVGVHPNDAGGFDGDSLSEIRALAQHPKVVAIGEIGLDNYRQ
                     TVAPERQRIAFEAQLDLALDIGRPVIIHSRSAIDDVMNTLARKACPERSRRVTQHATR
                     STGILHAFSGNFSAAQRVFECGFLIGIGGPVTFKRSESLRELVRSAPLDRMVIETDAP
                     FLTPEPRRGRRNEPAYVRLVAQRIALERRASVEEIAEQTTANAARLFGWTSDYDTATP
                     "
     gene            3281..4252
                     /locus_tag="AC162G_10715"
     CDS             3281..4252
                     /locus_tag="AC162G_10715"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR5076334.1"
                     /translation="MTQRHLEMMDLVQEDLQRVEDALRVLPMIDYEVVEEAIGHLLDS
                     GGKRIRPTLTLLASRLYPADLDKAVSLAAAVEMLHTATLVHDDLIDDSLMRRGSPTLN
                     AIWTPGTTILVGDYLFARAADLAAQTDNVRVMQIFARTLMTIVTGELGQMFDDGHSVS
                     REAYERRIYAKTASMFELATEAAGVLIHAPEDQIGVLRDFGRDLGMAFQIVDDVLDFT
                     GDAVEVGKPVGSDLRRGLVTLPVLHYLENHPDDRRIADALSNGKRDEQHIGDIVESIR
                     ASDSIRASLEDAREYTRRSQRALSYLPEGSARQAMWDIAEYVITRTR"
     gene            4249..5169
                     /locus_tag="AC162G_10720"
     CDS             4249..5169
                     /locus_tag="AC162G_10720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5076335.1"
                     /translation="MSIDLSIVVVNWNVRDLLRRCLASIYKSSMARLEVIVVDNASSD
                     GSAEMVRTAFPAAMLLSNADNVGFPAGNNQGFAAARGRYVMTLNPDTEIVGDALAEMT
                     AYLDAHRDVGALGPQLLNSDGSVQSSRRRFPTFATALFESTWLQGIAPPGVLARYYMD
                     DRPADVAQEVDWVTGACILVRRDVLASVGGFDEAFFMYSEELDWCRRIKLAGWKIVYL
                     PEAKVIHHVGRSSGQAVAARHVHFQTSKVRYFKKHHGRFAGGMLRVFLLALYAWQMLL
                     ESVKMLLGHKREMRRQRVRVYWQVVRSRLR"
BASE COUNT         1023 a         1620 c         1691 g          962 t
ORIGIN      
        1 gaaaacgcct ggactcaatc cacctcggcc aaggtgttca gcctcctgct gacgctgatg
       61 gccgaagccg gccagtaccg ccacgagacc taccggccgc cgctcgacgc cgaccagact
      121 cgctaccgca tgttgcagga ccaagtgttg ccgggcatgg gcagactccg cgcggtcgcg
      181 gtgaaactgg cgaagaaata tctctcgcgg cccgttttcg cctcggtcgc gcgcgacatc
      241 gaagaagaga ttttcccact gcttgacgat atggacccgg ccgtggcgcc ggaccgttat
      301 atgccttttc gcgtgatcca tgtcggcaac gtcgtcgagc ggctttacgg attccggttt
      361 cgtaccggcg atccgactct ggtgggcgat tcgaacggcg ctggattgtt gcaggaaatc
      421 tacgagcgga agtacctgcg tttcggcaca tccggcgtgc gtgggcgctg gggacgggat
      481 ttcaccgagg agcgagctaa gcaggtcgtg caggccatct gcgacttcct gaagaaccag
      541 gatgcgccac cgtacgcggc cgccgaaaac cttcagggca gacgcatggt gatcggctat
      601 gacagccgat tgaacgcgcg gctggtcgcg tcctgggcag ctcaggtgtg cctggcggac
      661 ggtttctccg tcgaccaggc caaccgggac actccgacgc cggcgcttgt ctattacatg
      721 accgactacc ttggcgcgga cgaagtggct ggcctgatca actgcaccgc cagccacaac
      781 ccgcctgaat ggcaagggat caagttcaac ccgcgcctgg gctaccctgc gccgactaac
      841 ctgacggact tcatcgcctc gcggatcaac gagatccaaa tcgtgggccg acgaaacacg
      901 gaggcggcac tcgaggacca gaccgaacag gggcggctgc gaggattcga tccgatcgat
      961 ttgtatacca actggatact cgacagcggg cgcggcaacc cgcgcatcgc gccagacttt
     1021 gatcgaatcc gcaagtattt cggcgatcaa gtggtggtgg tggacgagat gcacggcgca
     1081 ggccgcggct acctgacacg tctcctgggc gagatcggtg tgcgctgcac ggtgattcac
     1141 gccgagaccg accctatgct gcccgggctg gactatgcca acccagagga gccgtttatc
     1201 aattccctca aggaaaaggt gaaagaatcg ggggcgatcc tgggcctcgg gctggatacg
     1261 gatgccgatc ggttcggcat tgtcgatcgg ggtggcatct acctccgacc caatcagatc
     1321 ctgcccatac tggtgcgata cctgggcgtg gatcgtggcc tcaacgggcg cgtgatcgcc
     1381 acgcagaccg gctcgccgct gatcgagccg ctcgcgggaa tgatccgggg caacgaagcg
     1441 ttcatgccgg aaagcggcgt gatcccggcc tacatcgatc accctttcta ccagcggaga
     1501 gtgggccagc gcgaggatcg cgtttacaag aacacgttta tggtgccggt tgggatcaag
     1561 tacattgaag aacagcggcg cacagacaga cgctatcgga gcctccccca gctcttggac
     1621 aactggcgga gcgtcctgtt gattggcggc gaggaaagct caggtttgac cacgcgcggt
     1681 cacgtgacgg acaaagatgg catctgggcg aatctgctgg tgatggacat gctggcctac
     1741 tacggcacgc tcccggataa gagcctgagt tcgctcagcg atatctggca agatacctgt
     1801 cagttgccgg gatgctggct ttcttacggt gggcgcgagt tgcgaggctc gaacgccggc
     1861 cgcgccgatg tcgacgccat cctggaggcc aaagaagact tgatcagcta ctatctcgat
     1921 ctgttttcgg cgaccaagcc ggacaacaag cctgccctga gcggagccga agggcttgcc
     1981 gatctgaacg tcgtgtatgc aggcggcgtt cgatatgaca tttgcgaact gcaactgcgc
     2041 gacgcccaag gcgatgaccg gcatttcctg cgcgtgcgct cgtccggcac agagccgatc
     2101 aaccgcatct atgtcgaaag cagcaatccc gagattgccc agcggttgat gcggaccgtt
     2161 ctggacaaac tggaagaact gatcgcccgc gagatcggcc aggctcagac tgagtggcgc
     2221 ttggtagatg tgcttacctt gacgcagatg tcgccgcgtc ttctggacac cgtgcagaag
     2281 gcactcgctg agcgcggttg gtcggtggcg agcgtcaccg gcaagttgaa gcgaataatc
     2341 catgcgccca actatttgga gcatcggaac agacgcacgg cgctgggctg ggtgaatgcg
     2401 ttggaatcac cctaggcgaa tttctccatc cgaattgtgg tgaagcagtc ctttaagggt
     2461 gcaaccggga tgatccgctt ggtcgatacg cattgccacc tcgattggca tacgttcgac
     2521 gcggatcgcg aagaggccat ttggcgcgcg gtcgacgccg gcgtgacgcg catggtgacg
     2581 gtcggcgtca gcgtggcgag cagccgcatg gccgtcgagc tggccggccg gtacgagccg
     2641 gtgtatgcgg ccgtgggcgt gcatcccaac gacgccggcg gcttcgacgg cgacagcctg
     2701 agcgagattc gcgccctggc gcagcatccg aaggtcgtcg cgatcggtga gatcggcctg
     2761 gataactacc ggcagacggt cgcgcctgag aggcaaagaa tcgcattcga agcgcaactg
     2821 gatctggcgc tcgacatcgg caggccggtc atcattcaca gccgcagtgc aatcgatgac
     2881 gtgatgaata cactggcgcg caaggcttgc cctgagcgaa gccgaagggt cacccaacac
     2941 gcaacacgca gcacgggtat ccttcacgcc ttctcgggaa acttcagcgc cgcgcagcgg
     3001 gtgtttgaat gtgggttttt gatcggcatc ggcgggccgg tcaccttcaa gcgatccgaa
     3061 tcactgcgag agctggtacg ctccgcgccg ctcgaccgca tggtgatcga gaccgatgct
     3121 ccgtttctga cgccggagcc tcggcggggc cggcgcaatg agccggcgta cgtgcggctc
     3181 gtcgcccagc gtatcgcgct cgaacgacgt gcctcggtcg aagagatcgc cgagcagacc
     3241 acggcgaatg cggcgcgcct gttcggctgg acaagcgatt atgacacagc gacaccttga
     3301 aatgatggac ttggttcagg aagatttgca gcgcgtcgag gacgcgcttc gtgtgctgcc
     3361 gatgatcgac tacgaggtcg tcgaggaagc catcggccat ctactcgaca gcggcggcaa
     3421 gcgcatccgg ccgacgctga ccttgttggc cagccggcta tacccggccg acctggacaa
     3481 ggccgtctcg ctggcagcgg ccgtcgaaat gctgcatacg gccacgctgg tccacgacga
     3541 tttgatcgac gactctctga tgcggcgcgg cagcccgacg ctgaacgcaa tctggacgcc
     3601 gggcacgacg atcctcgtcg gcgactacct gttcgcgcgc gcggccgact tggccgcaca
     3661 gacagacaac gtgcgagtca tgcagatctt cgcccggaca ctgatgacga tcgtcacggg
     3721 tgagctggga cagatgttcg acgacggcca ctccgtgtcg cgcgaggcat atgagcggcg
     3781 catttacgcc aagacggcgt cgatgttcga gctggcgacc gaggcggccg gcgtgctgat
     3841 ccatgcgccc gaagaccaga tcggcgtgct gcgcgacttc ggccgtgacc tgggcatggc
     3901 tttccagatc gtcgacgacg tgctcgactt caccggcgat gccgtggaag tcggcaaacc
     3961 ggtcggcagt gacttgcgcc ggggtctcgt gacgctgccg gtgcttcact acctggagaa
     4021 ccatccggat gaccgccgca tcgccgatgc actgagcaac ggcaagcgtg acgagcagca
     4081 catcggcgac atcgtcgagt cgatccgcgc ctcggattcg atcagggcca gcctggagga
     4141 cgcgcgcgaa tatacgcggc ggagccagcg cgcgctgagt taccttccgg aaggctctgc
     4201 ccgccaggcg atgtgggaca tcgccgaata cgtcatcacc cgcacccggt gagcatcgac
     4261 ctctcgatcg tcgtcgtcaa ctggaacgtg cgcgacctgc tgcgccgctg cctggcgtcc
     4321 atctacaagt cgagcatggc gcggctcgaa gtcatcgtcg tcgacaacgc gtcgagcgat
     4381 ggcagcgcgg agatggttcg caccgcgttc cccgccgcca tgctgctttc caatgccgac
     4441 aacgttggct tcccggccgg caacaaccag ggcttcgccg ccgcgcgagg ccgctacgtg
     4501 atgacgctga acccagacac agagatcgtc ggcgatgcgc tggctgaaat gaccgcgtac
     4561 ctcgacgcgc atcgtgacgt cggcgcgctc ggcccgcaat tgctcaattc ggatggatcg
     4621 gttcagtcgt cgcgtcgccg cttcccgacc tttgcgaccg cgctgttcga aagcacctgg
     4681 ctgcaaggca tcgcgccacc cggtgtcctg gcccgctact acatggacga caggccggcc
     4741 gatgtggccc aggaagtcga ctgggtcacc ggcgcgtgca tcctcgttcg gcgcgatgtc
     4801 ctggcgtcgg tcggcggttt cgacgaggcg ttcttcatgt actccgaaga gctggactgg
     4861 tgccggcgca tcaagttggc ggggtggaaa atcgtctatc ttcccgaagc gaaggtgatt
     4921 catcacgtcg gccggagcag cgggcaggcc gtcgccgcgc ggcacgtcca cttccagacg
     4981 agcaaggtgc gctatttcaa aaagcaccat ggccgattcg caggcggcat gcttcgggtg
     5041 ttcctgctgg cgctgtacgc gtggcagatg ctgctcgaat cggtcaaaat gctattgggc
     5101 cacaagcgtg agatgcgtcg ccaacgcgta cgcgtgtatt ggcaagtcgt tcgttcgaga
     5161 ttaagataag gccacgagca cgcagtgcgc caccagaatt tgcaccacca ggatactgct
     5221 cagcgcgcac tgcgagtcct ccttttccta agactgacct ccccgaccac gttcaactgc
     5281 tgcgtgccga tattca
//
LOCUS       DBNHXB010000262         5552 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3924774, whole genome shotgun sequence.
ACCESSION   DBNHXB010000262 DBNHXB010000000
VERSION     DBNHXB010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3924774"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="AC162G_10725"
     CDS             <1..336
                     /locus_tag="AC162G_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR5076336.1"
                     /translation="NRGPFDVNGGAELVNNNGTSFGQAYPDDPDDPAPEFTSIFQSRG
                     GPSLRQIVDMGNLDASRFIHTTGQSGLPYHPHYDDMIQRWLNIEFAPMWWERTSVEQN
                     AEGTLSLTP"
     gene            641..1837
                     /locus_tag="AC162G_10730"
     CDS             641..1837
                     /locus_tag="AC162G_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076337.1"
                     /translation="MLTRRLLTVLILAALLITAFPLAASAQQPSTYAAIAVTGVTSGT
                     PAVGSNFTTDVQVTINTTGPGLVGMELYLSINPALVNAVDTDSSLPGVQPAELRSDFF
                     GVNPVPAANEVIVAPDTIAPSITVPGRTCPGGVYPCIHLSLVGPAQTNKTGTVARLHW
                     TGVAIGFAVFTILNPVIAPPPFPLSPRTALSDADGFLIPISSFSATGVSIVQGGTIGG
                     IVDRQGVPSDVGPGTDGCTQIDATNGAVVAVPGFTLVAPPPDLPAQVNTAGGFRITLP
                     TAGTYTVRASYPGYLQAQKSNVFASAGQALIGTTRLHGGDVNGDNAVNILDIVSIIGR
                     LGTAGLPVRSSAAVCIAPWEGSQTSVPPPPDSPFDINDDGTVDISDLSITAGNFGRVG
                     PTNWAP"
     gene            2057..3517
                     /locus_tag="AC162G_10735"
     CDS             2057..3517
                     /locus_tag="AC162G_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013156.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR5076338.1"
                     /translation="MPNPRLRSIRLPLAHRAPPVVSWSVVLVCLGALALVSLFAAPRA
                     GAQGGTVVRIEPASSSVQCGVTIVVDVRIENASNLAGAEVHLTYDPFLLDAQIQPGGF
                     PAPDYVLQSSATGGRIDFAITQMPPQAPVSGSGVLLKITLKGLASGVSAIDFTSALLS
                     DGAGVLIPSTTQNGSVGVCPTPTPTFTATHTPTPTYTPTPTHTSTPTPTTTSTPTSTP
                     TTVPGSAKALVSPPSSTVVVGNSTTVAIRIEGATNLWGVDLKLTYDPAIVECTASLPG
                     VIPKPSVLAKNSCGAGAAEYIAAQQAPDPPASGSGDAVRLTFKCLKEGTSPLHLERSK
                     LVDRDGRDLPNTATHGQITCTPPPDDGSILGYHKVRAGETLYCIGRAYRVNPWAIATV
                     NHIAYPYYVYPGQMLAIPDAPWTNIPPGPTCAAQFPVASPPPSPAGCRVVYVVKPGDT
                     LYKIAGMYGSDPWTIAARNNIYNLNLIYPGQRLCIP"
     gene            3684..4769
                     /locus_tag="AC162G_10740"
     CDS             3684..4769
                     /locus_tag="AC162G_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013156.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR5076339.1"
                     /translation="MRSNSAWNLTGITAGLVVTLALFGSAFAQGGTVVRVDPAAATIA
                     PGQIVSISIKVDNVSNLFGAEVHLAFNPNVLEVIDSDAGQAGVQITNGGMLTPDFVTQ
                     NQADNTIGTIDFSVAQIGRPAVSGSGTLAIISFRGKAAGSAPITFRGTPAAPSGVILS
                     DVSAVQIPSTTQNGLVTVTAAVVTPSSTPTLTPTPIGPTFTPTPIPPSPSSTPTPTPT
                     GPTQTPTPSATPGGGTPGRHVVRAGETLFCIGRAYGVLPWAIAATNRIFAPYRLFPGQ
                     VLTIPNVPWSNIPAGPTCARQFPGTPPPPPPPPPPPACRAIHIVRPGETLWRISRIYG
                     TNPWAIGAVNNIFNLNLIYPGQRLCIP"
     gene            complement(4827..>5552)
                     /locus_tag="AC162G_10745"
     CDS             complement(4827..>5552)
                     /locus_tag="AC162G_10745"
                     /inference="COORDINATES: protein motif:HMM:NF048886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkZ-related protein"
                     /protein_id="HIR5076340.1"
                     /translation="IQGIEMPSLWAAVAGDRPVADEHDDPGHITWNWKDQSLDNRRWY
                     YGKLLRGKATLVSLDTLPYFYALSENFGGADDYLEEYRAGRLSAEAKTIYEVIRDGGA
                     MDTVRLRREARMAAESSKARFEKALTDLQKGLKILPVGVAEAGAWRYAFVYELLDRWF
                     PDAAEAARLITRGAARTALARHYVENVVVATPKAIARLFGWRKDDLEAAIATLVERGA
                     FITDIQVEGARGDHIAAATLLKR"
BASE COUNT          994 a         1821 c         1691 g         1046 t
ORIGIN      
        1 aaccgtgggc cgttcgacgt gaacggcgga gccgagctgg ttaacaacaa tggcaccagt
       61 ttcggccagg cgtatccgga cgatcccgac gaccctgcgc ccgagttcac gtctatcttc
      121 cagtcgaggg gcggcccgtc gctgcgccag atcgtcgaca tgggcaatct cgacgcgtcg
      181 cgcttcatcc acaccaccgg ccagagcggc ctgccgtacc atccgcatta cgacgacatg
      241 atccagcgct ggctcaacat cgagttcgcg ccgatgtggt gggagcgcac gagcgtcgag
      301 cagaatgccg aaggcacgct cagcctgacg ccgtgatggc ggatagcaga tggcggaggg
      361 cagatagctg atagctgatg gcggaatggc gaacgtcaaa cgttcgtcct tcgtactgcg
      421 gtgtctctgc aagacccggc tcagaggttg gagccgggtt ttgtttgcaa atggtatatg
      481 gatgaagcgc ttcgcccctc tggcaattca aaccggaact tggcagtaac ctaaccggaa
      541 ctcacagtgc gcgtggtttg tgctactctg ggtttgacgt gtactcatcc gtctcgagaa
      601 ggattccgct gcaacgatct gtatggaaag gaacatctac atgttgaccc ggcgcttgct
      661 aaccgtcctt attctcgcgg cactgctcat cacggcattc cctctggcgg ccagtgccca
      721 gcagcccagc acgtacgccg caatcgcggt gaccggcgtc acctccggga cgccggccgt
      781 gggcagcaac ttcacgactg acgttcaagt cacgatcaat accaccgggc ccgggctggt
      841 cggcatggag ctatacctgt ccatcaatcc cgctctggtc aacgcggtgg acacggacag
      901 ctcgctgccc ggcgttcagc ccgcagagtt gagaagcgac tttttcggag tcaaccccgt
      961 cccggctgcg aacgaagtga tcgtcgcccc cgacaccatc gcgcccagca tcacggtgcc
     1021 cggccgcacc tgccccggag gcgtatatcc gtgtatccat ttgtcactgg taggaccggc
     1081 gcagacgaac aagaccggca cggttgcccg cctgcactgg actggtgtcg ccattgggtt
     1141 tgccgtcttt acgatcctga acccagtcat tgcgccgccg cccttccctc tgtcgccacg
     1201 cacggcactg agcgacgcgg acggcttcct cattccaata agctctttta gcgcgactgg
     1261 tgtgtctatc gtgcagggag ggactatcgg cggcatcgtg gaccggcaag gtgtgccttc
     1321 ggacgtgggc ccaggcacgg acggctgcac gcagatcgat gcgacgaatg gcgccgtcgt
     1381 cgccgtgccc ggattcacac tggtagcccc gcctccggat ctgccggcgc aggtgaacac
     1441 ggcaggcggc ttccggatta cactgcccac cgccggcacg tacacggtgc gcgcgtcgta
     1501 tcccggctat cttcaggcgc agaagtccaa cgtgtttgcc tcggctggac aggcactcat
     1561 cggcacaacg cgcctgcacg gcggcgatgt gaatggcgat aacgccgtca acattctcga
     1621 catcgtctcc atcatcggtc gcctcggcac tgccgggctt ccggttcggt cgtctgcagc
     1681 cgtctgcatc gcgccttggg aaggttcgca gacttccgtg ccgccgccgc ccgatagccc
     1741 cttcgacata aacgacgacg gcacggtgga catcagtgat ctgtctatca cggcgggcaa
     1801 cttcggtaga gtcggcccga cgaactgggc gccgtagtca tttgccgctt cgactcgccc
     1861 cccggcttcg aggagaagcc gggggctgaa gtcgcacagg ggatgaacca atgcagcgtc
     1921 tcatatggct gacggtcttg ctctctttgg ccgtcttcgc cgcagcgccg tgttagtccc
     1981 cgcgcacttg cgcgtcgagt ttcaccttca ctgcctgagc ctgtgggact agcaagaagg
     2041 gcagaaggag ggcgccatgc ctaacccgcg attgcgatct attcgattgc cgctcgctca
     2101 tcgcgcgccg cctgtcgtgt cgtggagtgt cgtgctcgtc tgcttaggcg cgctcgcgct
     2161 ggtgagcctg ttcgcggcgc cgcgcgcggg cgcgcagggc gggacggtcg tgcggatcga
     2221 accggcaagc agctccgtcc agtgcggtgt gaccatcgtc gtcgacgtgc gcatcgaaaa
     2281 cgcgtcgaac cttgccggcg ccgaggtgca tctcacctac gatccattcc tgctggatgc
     2341 gcagattcag ccgggtggct tccctgcgcc agactacgtg ctacaaagca gcgcgaccgg
     2401 cggccggatc gattttgcca tcacgcaaat gccgccgcag gctccggtca gcggcagcgg
     2461 cgtgctgctc aagatcacct tgaagggtct ggcctccggc gtgtcggcga tcgattttac
     2521 gtcggctttg ttgagcgacg gagccggcgt tctcatccct tcaacgacgc agaatggatc
     2581 agtcggtgtg tgccccacgc cgactccgac gttcacggcg acccatactc ctacaccgac
     2641 ctatactcct acgccgaccc atacctccac gccgacgccg accacgacgt ccacgccaac
     2701 gtcgacgccc acgacagtgc ccggcagcgc caaagcactc gtgtcgcctc cgtcgtccac
     2761 agtggttgta ggcaattcca ccacggtcgc catccggatc gaaggggcga cgaatctgtg
     2821 gggagtggac ctcaagctaa cctacgatcc ggcgattgtc gaatgcacag cctcactgcc
     2881 cggcgttatc ccgaagccga gcgtgctggc gaagaattcg tgcggcgccg gcgcagccga
     2941 gtacatcgcc gcccagcagg cgccagaccc tccggcaagc ggcagtggcg atgcggtgcg
     3001 cttgaccttc aaatgcctaa aagaaggaac atcgccgctg caccttgaac ggtcgaagct
     3061 ggtggatagg gatggccggg acctgccgaa cacggccact cacgggcaga tcacctgcac
     3121 accgccaccg gatgatggtt cgattctagg ctaccacaag gtgcgggctg gtgaaacgct
     3181 gtactgcatc ggccgcgcct acagagtgaa tccatgggcc attgcgacgg tcaaccacat
     3241 cgcgtatccg tattatgtat acccgggcca gatgctagcg atcccggatg caccgtggac
     3301 gaacattccg ccgggtccga cgtgcgcggc tcagtttccg gtcgcttcgc cgcctccgtc
     3361 tccggctgga tgtcgtgtcg tgtatgtggt gaagccaggt gacacgttgt acaagatcgc
     3421 aggcatgtac ggcagcgatc cgtggacaat agctgccagg aacaatatct ataatctgaa
     3481 cctgatctac cccggacagc gtctgtgtat cccgtagccc tgttcttgag tttggctgaa
     3541 gcgcggcaaa tgtggtagac tctggcacac tccagcgcgc acggcggtcg cggtcgcagc
     3601 gaagatgcac ggactgtgtg ctgccgtgtg acgagacgca atcgatccgt tgatcaggct
     3661 gcaatccaac accgaggtgg attatgcgaa gcaacagtgc ctggaacctg acaggtatta
     3721 ccgccggact ggttgtcacg cttgcgctat tcggctcggc gttcgcgcag ggtggaacgg
     3781 tcgtgcgtgt cgatccggct gcggcgacga tcgcaccggg acagatcgtc tcgatatcga
     3841 tcaaggtcga caacgtgtcc aacttgtttg gcgccgaggt ccatctggcg ttcaatccga
     3901 acgtgctcga agtgatcgac tcggacgccg gccaggcggg cgtgcagatc accaacggcg
     3961 gtatgctgac ccccgacttt gtcacgcaga accaggccga caacacgatt gggaccatcg
     4021 acttcagcgt ggctcagatc ggccgcccgg cggtgagcgg aagcgggacg ctagccatta
     4081 tcagctttag gggcaaggcg gcggggtcgg cgccgatcac tttccgtggt acgccggctg
     4141 ccccctcagg cgtcatccta agcgatgtaa gcgcagtgca gattccgagc acgacgcaga
     4201 atgggttggt gacggtgacc gccgccgtgg ttactccgtc ctcgacgccg acgctgacgc
     4261 ccacgccaat cgggccgact ttcacgccga cgcctattcc gcccagccca agctcgacgc
     4321 cgacacccac gccaaccggt cctacgcaga cgccgactcc atcggcgacg cccggtggcg
     4381 ggacaccagg ccggcatgtc gtgcgagcgg gcgagacgct gttctgcatc ggccgcgcct
     4441 atggcgtgct gccctgggca attgccgcga cgaatcgcat tttcgcgccc tatcggttgt
     4501 tccccgggca agtgctgacg atcccgaacg tgccctggag caacatcccg gccggcccga
     4561 cctgcgcgcg ccagttcccg ggtacaccgc cgcctccgcc tccgccgcct ccgccgcccg
     4621 catgccgtgc catccacatc gtgcggcccg gcgagacgct gtggcggatt tcacgcatct
     4681 atggaaccaa tccgtgggcg attggggccg tgaacaacat cttcaacctc aacttgattt
     4741 accccggcca acggctgtgc atcccgtaat tgctatagac ctggcaggtc tctaagacct
     4801 gccaggtctg ccaagagacc ggggttctat cgtttgagca gcgtcgcggc ggcaatgtga
     4861 tcgccgcgcg cgccctcgac ctggatgtct gtgataaacg cgccccgttc gaccagcgtg
     4921 gcgatcgccg cttcgagatc atccttgcgc cagccaaaca gccgggcgat cgccttcggc
     4981 gtggcgacga cgacgttctc cacgtagtgt ctcgccagcg cggtgcgtgc tgcgccgcgc
     5041 gtgatgaggc gcgccgcttc cgccgcatct gggaaccacc gatcgagcag ctcgtagacg
     5101 aaggcgtagc gccacgcgcc cgcttccgcc accccgaccg gcaggatctt cagccccttc
     5161 tgcaaatccg tcagggcctt ctcgaatcgc gctttgctcg actcggccgc catgcgcgcc
     5221 tcgcgtcgaa ggcgcacggt gtccatcgcg ccgccgtcgc ggatcacttc gtagatcgtc
     5281 ttcgcttccg ccgataaacg gccggcgcgg tactcctcga gatagtcatc cgcgccaccg
     5341 aagttctcgc tgagtgcgta gaagtacggc agggtatcca gtgagacgag ggtggctttg
     5401 ccgcgcagca gcttgccgta gtaccatcgg cggttgtcga gcgactgatc tttccaattc
     5461 caggtaatgt gacccggatc gtcgtgctcg tcggcgacgg gtcgatcgcc ggcgacggcg
     5521 gcccacaggc tcggcatttc gatgccctgg at
//
LOCUS       DBNHXB010000263         6099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1409210, whole genome shotgun sequence.
ACCESSION   DBNHXB010000263 DBNHXB010000000
VERSION     DBNHXB010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1409210"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="AC162G_10750"
     CDS             <1..1169
                     /locus_tag="AC162G_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR5076341.1"
                     /translation="DVGVREGEAPVEAGGAVQLMTVHKSKGLEFPVVVIADAGYEPRG
                     GTDSVLIDDDLGVLIGVSNSDGARPVAWRLASLKEAAKEDAEDRRLLYVAATRAKEKL
                     VVSGHAKRTAKGALSLSGWLKRLGSVIGLDEVTLDGEITTPRALEVRCPGNAGPIACV
                     LHPPRAAERSTVQVVRIPAPAAAPLTVPDLVAPLLTGGIEADHQPSAISYSLSRVWRV
                     VPRAKRPSGPAWVVGKLVHEALRRWRFPDEDNFDVFLWPFALETGLADAVEIQATIRE
                     ARRLLSHFQGHTLFAEIAAAERYHEVPYALPDDHGVIDLLYHTDAGWTVVDFKTDELR
                     SEAEMREAIPREGYDVQLRRYADAVTAQLGHRPKALLVFLRVAGEVHVMELVGS"
     gene            1665..4859
                     /gene="fdh"
                     /locus_tag="AC162G_10755"
                     /pseudo
     CDS             1665..4859
                     /gene="fdh"
                     /locus_tag="AC162G_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
     gene            4969..5796
                     /locus_tag="AC162G_10760"
     CDS             4969..5796
                     /locus_tag="AC162G_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5076342.1"
                     /translation="MPKGMFVDTSLCIGCKACQVACKEWNELEGEPDHFQEVNGRLKA
                     VNFTGNSYDNTGALSATDWRHVRFIERGSSLEGNMAWYMMSDSCKHCVQAGCLEVCPT
                     HALRRTDLGNVVVDQDVCNGCRACVAACPYGVISYSTRTGTVHKCTLCDDRIHNGLET
                     ACAKACPTDSIVFGEVDELRVNARKRVDTLKQLGFGKANLYGDTDILGGLNVFYLLLD
                     EPEIYGQPRNPQLPQRNLIPGSLWSIASAIALGIAALIAFRNRGAEVDARKEPDHAR"
     gene            5786..>6099
                     /gene="nrfD"
                     /locus_tag="AC162G_10765"
     CDS             5786..>6099
                     /gene="nrfD"
                     /locus_tag="AC162G_10765"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR5076343.1"
                     /translation="MPGELQIPQWEWWIVLYFFAGGIAGGAYLTSAIVELVGRPEDRP
                     IARMGYYIAFPLSLVCVVALIADLGRPERFWHMIFYSKTFQPWPKWDSPISVGAYALL
                     FFG"
BASE COUNT         1221 a         1961 c         1909 g         1008 t
ORIGIN      
        1 aggacgtcgg cgtgcgcgag ggcgaagcgc ctgtcgaggc aggcggcgcg gtgcagttga
       61 tgaccgtgca caagtcgaag gggcttgagt ttccggtcgt cgtgatcgcc gatgcaggct
      121 acgagccacg cggcgggacc gattcagtgc tgatcgacga cgacctcgga gtgctcatcg
      181 gcgtctccaa ctcagacggc gcccgcccgg tcgcctggcg cctggcgagc ctgaaggaag
      241 cggccaaaga ggacgccgaa gacaggcgtc ttctgtacgt ggccgcaacg cgcgccaaag
      301 agaagttagt cgtcagcggg cacgccaagc gcacggccaa aggcgcattg agcctgtccg
      361 gctggctgaa gcggctgggc agtgtgattg gactggacga agtcacgctc gacggggaaa
      421 tcacgacgcc gcgcgcgctc gaagtgcgct gtcccggcaa tgcagggccg atcgcctgcg
      481 tgcttcatcc gccccgtgcc gccgagcgtt caactgtgca agtagtgcga atccccgcgc
      541 ctgccgccgc gccgcttact gtccctgacc tcgtcgcgcc cctattgacc ggtgggatcg
      601 aggccgacca tcagccatct gccatcagct attcgctatc cagagtctgg cgagtcgttc
      661 cgcgcgcgaa acgccccagc gggccggcgt gggtggtcgg gaagctcgtc cacgaggcat
      721 tgaggcgctg gcgctttccc gacgaagaca acttcgacgt cttcctgtgg ccgttcgcgc
      781 tggagaccgg attggcggat gcggtcgaaa tccaggcgac gattcgcgag gcgcggcgct
      841 tgctcagtca cttccagggg catacgctct ttgccgagat cgccgccgcc gagcgttatc
      901 acgaggtacc ttacgcgctg ccagacgatc acggcgtcat cgatttgctg taccacacgg
      961 acgcaggttg gacggtcgtc gatttcaaga cggacgagct gcgctcggaa gccgagatgc
     1021 gcgaagccat tccacgcgag ggttacgacg tccagttgcg gcgctatgcg gacgccgtca
     1081 ctgcgcaatt gggccatcgc ccgaaagcgt tattggtgtt cttgcgcgtg gccggcgagg
     1141 tgcacgtgat ggagttggtg ggttcataat tcagcgctca acgcgtggtc ttccggcaaa
     1201 cctatccccc cattggcgcg tatttcaatc tgggaaccgt tccgtcgctt gagagttcag
     1261 cccatatttc gcacggctcg gcgtggattg gcaactccac gctccagccg ccggatttgg
     1321 cacgccctta ggcggcggaa gcgggtgcga aatgctggct cagcgcggcg tcctggaaaa
     1381 cgatagctac attgattatc acgcgtcgtg aaaattttca taaaattgac ttcactatta
     1441 aatagtaata agataggtta gctccctcag ccaagttgac tgtcaccaag tgacgtcgga
     1501 caacacagag taaggaatgc ccgggcagca cgcacgcgct gctttggggc tttctttcct
     1561 taatcatacg aaggaggtga tgcctatgcg ccgatagcga caaccaggcg tactgattgt
     1621 ggaacgagcg gatcgccttg aagttctgag aaggagaccc atctatggcg caacaactgt
     1681 cacggcgaca gttcatgaaa ctgtccgccg gagcgatggg tggtgcagtg gcagccgaga
     1741 tggccggcct cggcttcgac acttcacccc ttcacgctcg aactcacgcc atccggatca
     1801 agcagggcaa agaagtcccc agcgtttgtc cctactgcgc cgtgggctgc ggcgagatcg
     1861 tgactgtagc cgacggcaag atcgtcgaca tccagggcag ctacgacagc ccggtcagcg
     1921 ttggcacgct ctgccctaaa ggcgcggcta cctaccagct cgccgtcaat ccccaccgct
     1981 ggacgacagc caaataccgc gctcccaact ccgatcactg ggaggacatc gagctcgaaa
     2041 aggcgatgga catgatcgcc gagcggttca agaagacccg cgacgagagc ttcatcgaga
     2101 agacgatggt cggcgacacg gagaagaccg tcaaccaggt cctggccatc gcctcgctgg
     2161 gcggcgcgac gatcgacgac gagtggaact acctgcatca gaagctgctg cgagcgacgg
     2221 gcgtggtttc gattgaaaac caggcccgta tatgacactc cgccagtgtc cccggtctgg
     2281 ggataacgtt tggccgcggc gcggccacca ccttcgcccg cgatctatcg aactctgacg
     2341 ccgtcatgat catgggctcg aacatggcgg agtgccaccc cgtcgccttc cgctggccga
     2401 tgaaggccaa ggagaagggc gctaaaatca tgcacgtgga cccgcgcttc acgcgcacct
     2461 cggccatggc cgacgtccac gcgcccatcc gcgccggcac ggacatcgtc ttcctgggcg
     2521 cgctgatcaa caccatcatc aacagcgaac gctggaacag cgaccccttc ttcaaagagt
     2581 acgtcgtcaa ctacaccaac gccgccacgc tcattaccga agagtatcaa gaccccgagg
     2641 acctggacgg cgtgttctcc ggtctcgacg ctgaaaagcg ccggtacgat acccagtcgt
     2701 gggcctatca gcgcgaagag gcgctggtca aacctaccgg cgaagagaag acctacgccg
     2761 aaatggtcgc ggcgcgcgtg cccggccggt ccaagaccga cccaacgctg cagcatccgc
     2821 agaccgtctt ccaggtcatg aagcggcact acgcccgcta cacgccggag atggtcgaga
     2881 acgtgtgcgg ctgcccgaag gagaagttca ttcaggtggc cgagacgctg ctggcgaact
     2941 ccggccgcga ccgcaccagc gcgatcgtct acgccgtcgg ctggacgcaa cataccgtcg
     3001 gcgtacaatt cattcgcacc gccggtatgc tacaggtcct cctcggtaat gcaggtcggc
     3061 ccggcggcgg catcctggcc ctgcgcggcc acgcgagcat tcaaggctca accgatatcc
     3121 cgacgctcta ccactcctgg tcgggctaca tcaacgccgc cgacgcgcgc aagaaccacg
     3181 acaccctgaa ggatttcatc ctcaccgaaa cccagatcac cagcttctgg tcgaataccc
     3241 ccaagttcac cgtcagcctg ctcaaggcct ggttcggcga cgcggccacg ccggagaacg
     3301 acttcggcta caactccctg cccaagatca gcggcgacca ctcacacctg cccaccttcg
     3361 tgctgatgca cgaaggcggg gtcaagggct tcatcgccat cggccagaac ccggcggtgg
     3421 gcggccagaa tgccgaattc cagcgcacgg cgctgggcaa cctggaatgg atggtggtgc
     3481 gcgacttctt cgagaccgag acggctgcct tctggaagcg ccccggcgtg gacccgaagg
     3541 acataagcac cgaagtcttc ttcctgccgg cagcgcacgt tgccgagaac gagggcacct
     3601 tcaccaatac gcagcgcctg ctgcagcggc acgacaaggc cgtcgatccg cccgacgacg
     3661 cgcgcgccga tctgtggttc acctttcacc tgggccgtcg tctcaaggaa ctgtacaagg
     3721 acagcacgaa cccgcgcgac tggccgatcc agaatctgct gtgggattac ctgaatcccg
     3781 acgagaacgc ggagtggcgg atcaaggacg agccgagcgc cgacctggtg atgcgcgaga
     3841 tcaacggcta cacctgggcc gacaagaagc tcgtccccgg tttcaccgcg ctccaggacg
     3901 acggctcgac cgcctgcggc tgttggatct acagcggcgt gtatccggag gaaggcaaga
     3961 accgcgccgc ctcgcgcgag gccgacgact gggttagccc caattggggt ttcgcctggc
     4021 cagctaaccg ccacatcatg tacagccgcg cctccgccga cccggccggc aacccgtgga
     4081 gcgagcgcaa gaagtacacc tactgggatg cgaacaagga ctccggccag aaagacgcca
     4141 acggcaaccc gatcatcggt gtctgggtca acgccggagg agacgccatc gacttcccgg
     4201 tcaccaagaa gcctgacacg aaggcgaaag acggggctga gggcgtgggc ctgaacttcc
     4261 acgacggggt cagcccggcg atcatgaagc ccgacggcaa gttctggctg ttcgcgccga
     4321 gcggcgcagt ggatgggccg atgcccaccc actacgagcc gtacgaatcg ccggtcgcca
     4381 atcccgtgta caagcagcag cgcaatccag tcgccaagat ctggccggtc aagggcaacc
     4441 cgtaccacga gatggaagac gcggagaagt acccgatcgt cgtctccacc taccgcctga
     4501 ccgagcacta cctgtccggc acgatgagcc gctggctgcc ctggctggcg gaactgatgc
     4561 cggagctgtt cgtcgagatg agtccggaac tggcagaaga gaagggcgtc aagaacggcg
     4621 actgggtaac cgtcgtcacc gcccgcggcg aggtcgaagg ccgcgctctg gtgacgcgcc
     4681 gcatgcggcc cttcgtcatc gacggcaaga cggtgcacga gatcggcatc ccgtggcact
     4741 ggggttatca gggcctggcg agcggagacg tgaccaacga catcagcgcg ctcgtggccg
     4801 atccgaacgt gagcattcac gaaggcaagg tgttctcgtg caacctgcgg ccgggccgcc
     4861 gctccaagcc cgcgccggag ctgcaccagg agttgatcgc gcgccacacc gggccggaag
     4921 tgacggtggc aagcctgagc tgcaacgatt cgctcgggga ggtgtgcaat gcctaagggt
     4981 atgttcgtcg acacttcctt gtgcatcggc tgcaaagcgt gccaggtcgc ctgcaaggaa
     5041 tggaacgaac tggaaggcga gcccgatcac ttccaggagg tgaacggccg cctcaaagcc
     5101 gtcaacttca ccggcaactc ctacgacaac accggcgcgc tgtcggcgac cgactggcgg
     5161 cacgtgcgct tcatcgagcg cggctcgtcg ctggaaggta acatggcctg gtacatgatg
     5221 agcgactcgt gcaagcactg cgtccaggcc ggctgcctgg aagtgtgccc gacgcacgcg
     5281 ctccggcgca ccgacctggg caacgtcgtc gtggatcagg acgtatgcaa cggctgccgc
     5341 gcctgcgtcg cggcctgccc gtacggcgta atcagctaca gcacgaggac cgggacggtg
     5401 cacaaatgca cgctgtgcga cgatcgcatc cacaacgggc tggagacggc gtgcgccaaa
     5461 gcctgcccga cggactcgat cgtgttcggc gaagtagacg agctgcgcgt caatgcccga
     5521 aagcgcgtgg acacgctcaa gcagctcggc ttcggcaagg cgaacctgta cggcgacacg
     5581 gacattctcg gcgggctgaa cgtgttctac ctgctcctcg acgaaccgga aatatacggc
     5641 cagccgcgca atccgcagtt gccgcagcgg aacctcatcc ccggatcgct gtggagcatc
     5701 gccagtgcga tcgcactggg tatcgcggcg ctgatcgcct tccgcaatcg cggggcagag
     5761 gtcgacgcga gaaaggagcc tgaccatgcc cggtgaactg caaatcccgc agtgggaatg
     5821 gtggatcgtc ctgtacttct tcgccggcgg catcgccggt ggggcgtacc tgacgtcggc
     5881 tatcgtcgag ctggtcggcc ggccggaaga ccggcccatc gcccgcatgg ggtactacat
     5941 cgcctttccc ctgtcgctgg tgtgcgtggt cgcgctcatc gccgacctgg gccgtcctga
     6001 gcgcttctgg cacatgatct tctacagcaa gacgttccag ccgtggccca agtgggactc
     6061 gccgatctcg gtcggcgcgt acgcgctgct gttcttcgg
//
LOCUS       DBNHXB010000264        13105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3422558, whole genome shotgun sequence.
ACCESSION   DBNHXB010000264 DBNHXB010000000
VERSION     DBNHXB010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 13105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13105
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3422558"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="AC162G_10770"
     CDS             <1..982
                     /locus_tag="AC162G_10770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HIR5076344.1"
                     /translation="EVFKSDLGGHPLLSNNFVRWLNFPLVKNKRWFHKNIVLLGDALH
                     TAHFSIGSGTKLALEDAIALAGCFAGHREESSNSKSQTSKGSGQALLSPRPSPLLGAS
                     GVGSRRRVEAALAEFERVRMPVVEKFQDAALNSLTWLEDVQEHLHLEPVPFTYRLMTR
                     SRRISYNRIKRGDPEFIARYDAWRAQQPSAGPIPAEFLDLFQKKTFAHLATLMADGTP
                     HVTSVYVDYDGQHILINSAQGRQKDINMEKRRHVAIEVPDPDNPNRFLAVRGPIVDIT
                     EQGADEHLDRLAQRYLNRDKYPDSMRFPGEVRCIYKILPKHVTVWDPFGR"
     gene            complement(979..2274)
                     /locus_tag="AC162G_10775"
     CDS             complement(979..2274)
                     /locus_tag="AC162G_10775"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR5076345.1"
                     /translation="MRYFDQATEIMPRERLAVLQLEKLQAMLAELWGRNGFYTDKLKK
                     AGVGPDEVRTLANLSRLPLTTKHELTADQAAHPPFGTNLTYPPEAYIRYHQTSGTTGA
                     PLRVLDTEESWDWWGRCWGYVLSGAGLTAADRLFSAFAFGPFIGFWSAVEGARKIGAM
                     MIPGGGRDSPQRLELMRESGATALCCTPTYALRLAEVAREKDFDLHALPVRATIHAGE
                     PGANVPATKRRIEESWGAKCYDHAGASEVGAHSFECEAQPGGIHIIESEFIAEVLDPA
                     TGQAVEPGARGELVITNLGRLGFPILRYRTGDVVQLNTEPCICGRTFARFQGGILGRA
                     DDMITVRGVNVFPSAVENIIRRFNEVDEFRVTVYMVRHMDEMDVEVELVEDADPNTVA
                     AIGQAIDTMLAFRPNVRPVARGTLPRFELKAKRFHVQRG"
     gene            complement(2396..3136)
                     /locus_tag="AC162G_10780"
     CDS             complement(2396..3136)
                     /locus_tag="AC162G_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR5076346.1"
                     /translation="MTPLVYLVRHGEVEHHRTDVNLTPRGRAQAETTGAALADPLAEG
                     DTVLLYHSPVKRVKETADLMHASIDAALKTSGRDGRVHLHAPRQDQALCNVRFFAAPG
                     EEPEEPSLLYARINTPAFQQDLPPARADFYRGFWVSADPMGYWLTHDSAGGAETVEVV
                     LARLQGRLRSLFDPERLGETPSPRGRVHWIGITHSGAMRALLRAAFGDDPGEPDFCES
                     ILIEPSDRTGWVTLSYRGRSASLNIAYR"
     gene            complement(3133..3594)
                     /locus_tag="AC162G_10785"
     CDS             complement(3133..3594)
                     /locus_tag="AC162G_10785"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR5076347.1"
                     /translation="MTKTTSELPEKLLTWLRSGGPALLLTMGADGFPNVAYTWAAAPD
                     ARRVRFGADHGSATLANLQRDGRVALQIVGPENLVFLVKGTTRMLKERIAAAPFRIAL
                     MEMVVTETKDQSWPGVIVAPLAYEWPADQREAMRAMEQAVYIELREAEARA"
     gene            complement(3647..3994)
                     /locus_tag="AC162G_10790"
     CDS             complement(3647..3994)
                     /locus_tag="AC162G_10790"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5076348.1"
                     /translation="MTVTRLSLLREQLKPISSEMSAITPFRGEGYRAGLVAFRPLPAP
                     DDEFIRHAASDVIAHVLDGRGRLRLSHSEIDLEPGMLCHIPAGTAHDFVAEGHSPLLL
                     FYAVIDLEAPKLL"
     gene            complement(3991..4245)
                     /locus_tag="AC162G_10795"
     CDS             complement(3991..4245)
                     /locus_tag="AC162G_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076349.1"
                     /translation="MMPRDDWEQENAERLLEDVRRRRRALEDFVASLPSLLAAAPSDE
                     ARANIQPLADRLIQLVGNFHMNNLDEMELLRAEMEKLGKA"
     gene            complement(4314..4928)
                     /locus_tag="AC162G_10800"
     CDS             complement(4314..4928)
                     /locus_tag="AC162G_10800"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HIR5076350.1"
                     /translation="MTKRLIVGLSGASGVIYGIRLLEVLRGVDAPRVETHLVMSTAAR
                     QTILLETDYKVEEVEALASAHYRFNDIAAAISSGSFKTMGMVVIPCSMKTLSGIALSF
                     SDNLLLRAADVVLKDRRRLVLVPRETPLHLGHLRLMTQVVEMGAIIVPPMPAFYHRPQ
                     TVDDIINQTVNRVLDVLEIDLPADLFERWAGPRYAPSRREGPEP"
     gene            complement(4925..5488)
                     /locus_tag="AC162G_10805"
     CDS             complement(4925..5488)
                     /locus_tag="AC162G_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR5076351.1"
                     /translation="MAVQFCARCGTRLIFGPVEGRDREHCPACGYVVYHNPAPVGMAV
                     VEDRGRLLLIRRTIPPLKGYWAPPAGHVEIGESVPEAATRETKEEAGVDVVLDGLLGV
                     YSQADVKVVIIAYRGRVCGGEPKAGADADEVGLFAPGELPVQPPPDPLSATVMDRWFY
                     GVLQEVTAPWREARSPRLRGEPEGGRR"
     gene            complement(5511..6602)
                     /locus_tag="AC162G_10810"
     CDS             complement(5511..6602)
                     /locus_tag="AC162G_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR5076352.1"
                     /translation="MADEPYIIWLQNCDRTCLPTVGGKNASLGEMLRANIRVPPGFAV
                     TTAAYRDFLNHAGMAQEIQRILAGVDAENIAATEQASITIRQLMGETPIPARVEDAVR
                     SAYEMLAEQCGVPDLPVAVRSSATAEDLPGASFAGQQETTLWVRRAGVVLVRTAICWS
                     SLFTPRAISYRIKMGFPHEKVQISVGIQKMVHAHTAGVMFTLNPTNGDRSKVVIDASW
                     GLGETVVSGMVTPDNYVVDKVSRDILRRTISPKLIEYVPNPDGRQAEMCDVPPERQNS
                     LCLSDDEVLALVDLAKRIEAHYGAPQDIEWAIDRDLPFPSNVMILQCRPETVWSRKQA
                     APVAKGMSSALDYVVDNLLAGVRPRKSLP"
     gene            complement(6698..8527)
                     /locus_tag="AC162G_10815"
     CDS             complement(6698..8527)
                     /locus_tag="AC162G_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HIR5076353.1"
                     /translation="MSSTDGKFPGPFEVPTPPGCEGWEKLYPYYYLFSSERREFEEGK
                     FWFQDGMHHPEPLYPFDTITCESWWVALGQYNSRVFIVPPALGIDQRVLNGYLYISAN
                     ALTDEKVIGERVGHFMERAGYYYQHWDELYEQWKGKAEAVIRDLQGLKVPDLPEMEDI
                     SVVKEGKGVTSAYHLLEAYNRAIENMHKIWHLHFEFLNLGYAAYLTYYGFCKKAFPDI
                     SDQSIAKLVSGIDVILFRPDEELKRLAQAAVDLGVAGVFKKTVQPDEVIAEMQKSDAG
                     KKWLDELEKSKDPWFYFSTGTGFYHLHRSWIDDMTVPCLSIRGYVEKIEAGQDIARPL
                     GEIQKERDRLAAEYTALLQTEEDKKAFQEQLGLARTVFPYVENHNFYVEHWHHTIFWN
                     KMREFGRLFVKHGFFKETDDLFYLHRYEVQAALYDLIASWAVGTEARGPKYWPSIVEE
                     RKVLFEKLKAWAPPPALGEPPEVVTEPFTIMLWGITTDSIQRWLQPKAKPEDAKELTG
                     FAGSPGVVEGPARVITSVEELSDVQPGEILVCPITTPSWAPVFTRIKATVTDIGGMMS
                     HAAIVCREYGLPAVIGTGYGTKTFKNGQMLRVDGSSGVVTVLN"
     gene            complement(8796..9611)
                     /locus_tag="AC162G_10820"
     CDS             complement(8796..9611)
                     /locus_tag="AC162G_10820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HIR5076354.1"
                     /translation="MRPIEGIIFDIDGCVAKDKNAIPGVPDTLAELRRRGVRFTFLTN
                     DNQNTIGFWVERLNAMGIPARAGEIMTSALIAASVTRELHADRKILVVGAPGLKEAMQ
                     AQGLNLIDLDHATEAEVVVMGKDPEFNQKALNQVCQAIWKGAEFIATNEDARVPSADG
                     FIPATGPMVKAVAYATGKEPLVTGKPSKWAGAMGMKMLGVPPERGAVAGDQLKQDIAM
                     GKQAGLFTILVLTGSTTAEEAAAAPVGLRPDLVLPDVNRLPDWLDGRWPMARG"
     gene            complement(9687..11153)
                     /locus_tag="AC162G_10825"
     CDS             complement(9687..11153)
                     /locus_tag="AC162G_10825"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR5076355.1"
                     /translation="MPYRDLREFLDRLEAEDHLRRIYKEVDWNLELSHIAKVNEEEHN
                     GGKALLFENVKDYPGWRVLTSALTAKERLALALELPTDMSLMNLSRAWVDRIQGKRVP
                     PRVVNSGPVHENVVQGDDVNIFKLPAPWFYPGDGGRYLGTAGYLISRNLDTGRLNVGT
                     YRLMVVDEKRASIQMIKAKDSEIDLRGYAAKNIPMPVAYVCGAEPVLFLCGSTLFGLN
                     ESEYEIAGALRGEPVEVTQALTNDISVPATAELVIEGEIMPGETLQEGPFGEYTGHYS
                     GKEFPKEFIQIKAITYRNNPILWSTTVGKPITDTHMIMAVNRTASLWNDLKAMNIPGI
                     KAVYGPPASAGRMLVIISMKPMYHGHSTQVGLAAFATVTGNYGLKTVILVDDDIDPEN
                     MDQVIYALSFKYQPDFGTQIIRRGRSTPLDPSLPIGDTRYMTSRIIIDCTTPYEWKEK
                     PKPIFLDENMVKHVKEHWDEYFAMPADLPQRVMEMAQA"
     gene            complement(11389..12141)
                     /locus_tag="AC162G_10830"
     CDS             complement(11389..12141)
                     /locus_tag="AC162G_10830"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR5076356.1"
                     /translation="MADFQTIQKSRSIPEVVLEQVQKLIAEGKLQPGDHLPSEMDLAE
                     QFGVGRSSIREAMRVLQLVGVVEVIQGKGTFVREPGILPLMVDWSRISQMGIISEVME
                     ARQFMEVLLAQLAAERATEEDIEALRAALERSRASITNVETNVRAGVNFHLAIADAAH
                     NQVLALMYRTIHDLYLEAARRTRITPEAAHDRLHDHECILAAIIARDPETAAQVMKEH
                     IDKAYQIMSAGEPEKAGSPDLNLSHSRDFGSL"
     gene            complement(12378..12878)
                     /locus_tag="AC162G_10835"
     CDS             complement(12378..12878)
                     /locus_tag="AC162G_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR5076357.1"
                     /translation="MTEDHSSLHEKTLAYLREHNVMTLATAGPQGLWAAAVFYVNDGF
                     TLYFLSAPTSRHSLNVEAQPIVSATIQEDYRDWREIKGIQLEGEARRVEGAEQAAAMA
                     RYGSKFPIVGNLAQAPAEIVKAMSRIVWYRVVPAHLYFIDNSLGLGHRDEVSLPHIPN
                     ATGFSG"
BASE COUNT         2397 a         4160 c         3975 g         2573 t
ORIGIN      
        1 cgaagtcttc aagtcggatc tgggcggcca tcccctcctc tcgaacaact tcgtgcggtg
       61 gctcaacttc ccactcgtca agaacaagcg ctggttccac aagaacatcg tgctgctggg
      121 cgatgcactg cacacggcgc acttctcgat cggctcaggc accaagctgg cgcttgaaga
      181 cgcgatcgca ctggccggct gtttcgccgg acaccgcgag gaaagttcaa actccaaatc
      241 tcaaacttca aaagggtcag ggcaggctct gctaagtcct cgaccgagtc ccctgctggg
      301 ggcgagtgga gtgggaagcc ggcgaagggt cgaggccgcg ctggcggagt tcgagcgcgt
      361 gcgcatgccg gtcgtcgaga agttccagga cgccgccctc aacagcctga cctggctcga
      421 agacgttcag gagcacctgc acctggagcc tgtcccgttc acctacaggc tgatgacccg
      481 cagccggcgc atcagctaca accggatcaa acggggtgat cccgagttca tcgcgcgata
      541 tgacgcatgg cgcgcgcagc agccatctgc cgggccgatc ccggccgaat ttctagacct
      601 gtttcagaag aagaccttcg cgcatctcgc cacgctgatg gccgacggca cgccgcacgt
      661 cacgtcggtg tacgtggact acgacggcca gcacatcttg atcaactcgg cgcagggacg
      721 ccagaaggat atcaacatgg agaagcgacg gcacgtcgcg atcgaagtcc ccgatccgga
      781 caaccccaac cgcttcctgg ccgtgcgcgg gccgattgtc gacattaccg agcagggcgc
      841 cgacgagcac ctcgacagat tagctcagcg ctacctcaac cgtgacaaat acccggacag
      901 catgcgcttt cccggcgaag tacgctgtat ctacaaaatc ctgccgaagc atgtgacggt
      961 ctgggacccg ttcggccgct agcctcgctg cacgtggaat cgcttggcct tcaattcgaa
     1021 gcgaggcaaa gtgccgcggg cgacaggccg aacgttcggc cggaaggcga gcatcgtgtc
     1081 aatcgcctgg ccgatggctg caacggtgtt cggatcggca tcttccacca gctcgacctc
     1141 gacatccatc tcgtccatgt ggcgcaccat atagacggtg acgcggaact catcgacctc
     1201 gttgaagcgc cggatgatat tctcgacggc gctggggaag acgttgacgc cgcgcactgt
     1261 gatcatgtcg tcggcgcgcc ccagaatgcc gccctggaaa cgggcgaatg tccggccgca
     1321 gatgcacggc tcggtgttga gctgcacgac gtcgccggtg cgataacgaa gaatgggaaa
     1381 gccgagccgg cccaggttcg tgatcaccag ctcgcctcgc gcacctggct ccaccgcctg
     1441 tccggtcgcc gggtcgagca cctcggcgat gaactcgctt tcgatgatgt ggatgccacc
     1501 gggctgcgcc tcacactcga aggagtgcgc gccgacctcg gaagcgcccg cgtgatcgta
     1561 gcacttcgcg ccccagctct cctcgatgcg ccgcttggtc gccgggacgt tggcccccgg
     1621 ctcgccggca tgaatcgtcg cccgcaccgg cagcgcgtgc aggtcgaagt ctttctcacg
     1681 tgcgacttcg gccaaccgca gcgcgtaggt cggcgtgcag cacaacgccg tcgcgccgct
     1741 ctcgcgcatc aactccaatc gctggggtga gtcacgcccg ccgccgggga tcatcatcgc
     1801 cccgattttg cgcgcgccct cgaccgccga ccagaagccg atgaacgggc cgaaggcaaa
     1861 ggcgctgaag agccgatcgg cggcggtgag gcccgcgccg gagagcacat agccccagca
     1921 gcggccccac cagtcccagc tttcctccgt atccaatacg cggagagggg cacccgtcgt
     1981 gccggaagtc tgatggtatc ggatgtacgc ttccggagga tatgtcagat tcgtgccgaa
     2041 gggcggatgg gcagcctgat ccgccgtgag ctcgtgcttg gtcgtcagcg gcaggcgaga
     2101 gagattggcc agcgtgcgca cctcgtccgg cccgacgccc gcctttttca atttatcggt
     2161 gtagaaaccg ttgcgacccc acaactcggc cagcatcgct tgcagctttt cgagctgcaa
     2221 cacggcgaga cgctcgcggg gcataatttc ggtggcctga tcaaagtatc gcatgtggct
     2281 tatcgcttat cgcatatcgc tgatgttgat ggcgcgaggc gcatcgcgta tcgcatatcg
     2341 cacatcgctg tcttcggatc caggcaccgg gcgatcggcg actggcgata ggctatcagc
     2401 gatatgcaat gttcagagac gcactccggc cgcgatagga tagtgtcacc cagccggtcc
     2461 ggtcagatgg ctcgatgaga atgctttcgc agaagtctgg ctcgccagga tcgtcgccga
     2521 aagcggcgcg cagcagagca cgcattgctc cggaatgggt gatgcctatc cagtgtacgc
     2581 gtccacgcgg cgagggcgtt tcccccaacc tctcaggatc gaaaaggctg cgtaatcttc
     2641 cctgtaaacg cgccagaacg acttctaccg tttccgcgcc gccggcgctg tcgtgcgtca
     2701 gccagtagcc catcgggtcg gcgctcaccc agaagccgcg atagaagtct gcccgcgcag
     2761 ggggtaagtc ctgttgaaag gcaggcgtat tgatcctggc gtagagcagc gacggctcct
     2821 ccggctcttc acccggcgct gcgaaaaaac ggacgttgca caacgcctga tcctgccgtg
     2881 gcgcatgcag gtgaacgcgg ccatcccggc ccgatgtctt caatgcggca tcgatgctgg
     2941 cgtgcatcag gtcggcggtc tccttcacgc gcttcaccgg cgaatgatac aggagcaccg
     3001 tgtcgccttc ggccaggggg tcggcgagcg ctgctccggt cgtttccgcc tgcgccctgc
     3061 cgcgcggcgt gagattgacg tccgtgcgat gatgctccac ctcgccgtgg cgcaccaggt
     3121 agacgagcgg cgtcacgctc tggcctccgc ttcacgcagc tcgatgtaaa cagcctgctc
     3181 catcgcgcgc atagcctccc tttgatccgc cggccactcg taggcgagcg gcgcgacgat
     3241 cacgcccggc caggattgat ctttcgtctc ggtcacgacc atctccatca aagcgatacg
     3301 aaatggcgcg gcggcgatgc gttccttgag catccgcgtc gtgcccttga ccaggaatac
     3361 tagattttcc gggccaacga tttgtagtgc gacccggccg tctcgctgca ggttagccag
     3421 tgtcgcactg ccgtgatccg ccccaaaacg cacacggcga gcgtcggggg cggcggccca
     3481 cgtgtaagcc acgttgggaa acccatctgc gcccattgtc agcagcagcg ccggcccacc
     3541 ggagcgcagc caggtcagca gcttttctgg cagctcggac gttgtcttag tcacattccc
     3601 cctggattgc caaatccagg cccagatcgg ggattgggca atcccctcag agcaatttcg
     3661 gcgcttcgag atcgattaca gcatagaaca agagcagcgg cgagtgcccc tccgcgacga
     3721 agtcgtgcgc cgtgccggcc gggatgtggc agagcatgcc cggttccaga tcgatttcgg
     3781 aatggctgag gcgcagccgg ccccggccat ccagcacatg ggcaatgacg tccgaagccg
     3841 cgtgcctgat gaattcgtca tctggcgctg ggaggggacg gaaagccacc aacccggccc
     3901 ggtagccttc tccccgaaac ggcgtgatgg cgctcatctc ggagctgatc ggtttcaact
     3961 gctcgcgcag caggctcagg cgggtgacag tcatgctttg cccagtttct ccatttcggc
     4021 tcggagaagc tccatttcgt ccagattatt catgtgaaaa ttgcccacca actggatcag
     4081 gcgatcggcc agcggctgaa tgttggcgcg cgcttcgtct gacggcgcag ccgcgagcag
     4141 tgacggcaag cttgcgacga agtcttccag cgcgcggcgc cgccggcgga cgtcttcgag
     4201 aagacgctcg gcgttttcct gttcccaatc gtcacggggc atcatggctt acaaccctcc
     4261 cggcgttggg cgcccgcaag ggaacgcccc tacgatgtgg acctcacggg gcattatggc
     4321 tcaggtccct cccggcggct tggggcataa cgtggccctg cccagcgttc gaacaaatcc
     4381 gcaggcagat cgatctccaa cacgtcgaga acccgattga cggtttgatt gataatgtcg
     4441 tcgaccgttt gcgggcgatg atagaaggca ggcatgggcg gaacgatgat cgcacccatc
     4501 tcgaccacct gcgtcatcaa tcgcaaatgt ccgaggtgca gcggcgtttc gcgcgggacg
     4561 agcaccaggc ggcgacggtc tttgagcacg acgtcggcgg cgcgcagcag cagattgtcg
     4621 ctgaaagaga gcgcgatgcc ggagagggtc ttcatcgagc aaggaatgac gaccatgccc
     4681 atcgtcttga acgagccgga ggagatagcg gcggcgatat cgttgaagcg atagtgcgcg
     4741 ctggcgagcg cctccacttc ctccactttg tagtcggttt cgagaaggat ggtctgccgg
     4801 gccgcggtgc tcatcaccag gtgcgtctcg acgcgcggag cgtccacgcc gcgcaagact
     4861 tcgagcaggc gtatgccata aatcacgccg ctcgccccgg agagaccgac gatcagtctc
     4921 ttcgtcatcg gcggcccccc tccggctccc cccgcaggcg gggggagcgc gcttcccgcc
     4981 agggtgcagt gacttcctgt agtacaccat agaaccagcg atccatcacg gtcgcgctca
     5041 atggatcggg gggcggctgg accggcagct ctcccggcgc gaagagtccg acctcgtccg
     5101 catctgcccc cgctttcggc tcacctccgc acacccggcc tcgataagcg atgatcacca
     5161 ctttcacatc ggcctgtgaa tacaccccca gcaagccatc caggaccacg tccaccccgg
     5221 cttcttcctt ggtctcgcgg gtcgccgctt cgggcaccga ctcgccgatc tcgacgtgac
     5281 cggcgggcgg cgcccagtag cccttgagtg ggggaatcgt gcgccggatg agcagcaatc
     5341 gacccctgtc ctcgacgacg gccatgccga ccggcgccgg gttgtggtac acaacgtagc
     5401 cgcacgccgg gcagtgttcc cggtcacggc cctcaaccgg gccgaagatc aggcgtgtgc
     5461 cgcatcgcgc gcagaactgg acggccatcg ctcagtccct tcgccttcgc tcagggcagg
     5521 ctcttgcgcg gcctcacgcc cgccagcaag ttgtccacca cgtagtccag tgcgctcgac
     5581 atgcccttgg cgacaggtgc agcctgcttg cggctccaca ccgtttccgg ccggcattgc
     5641 aagatcatga cgttcgaggg gaacggcaaa tcacgatcga tcgcccattc gatgtcctgg
     5701 ggcgcgccat agtgagcctc gatacgcttc gccaggtcga ctagcgcgag cacttcgtcg
     5761 tcgctcaggc acaggctgtt ctgccgctcc ggcggcacgt cgcacatctc cgcctggcgg
     5821 ccgtccgggt ttggcacgta ctcgatcaac ttcggcgaga tcgttcggcg caggatatcg
     5881 cgtgacacct tgtccaccac gtaattatcc ggcgtcacca tgcccgacac gaccgtctcg
     5941 cccagtcccc aactcgcgtc gatgacgact ttggaacgat cgccgttggt cggattgagt
     6001 gtgaacatca cgccggccgt gtgcgcgtgg accatcttct gaatgccgac gctgatctgc
     6061 accttctcgt ggggaaagcc cattttgatc cggtagctga tggcgcgcgg ggtgaagagg
     6121 ctcgaccagc agatcgccgt gcgcaccaaa acgacccctg cccgacgcac ccacagcgtc
     6181 gtttcctgct ggcctgcgaa cgaggcacca ggcaggtctt cagcggtggc gctggagcgc
     6241 acggcgaccg gcagatcggg cacgccgcat tgctcggcca gcatttcgta ggcgctgcgg
     6301 acagcgtctt cgacgcgcgc cgggataggc gtctcgccca tcaactgccg gatggtgatg
     6361 cttgcctgct cggtcgccgc gatgttctct gcgtccacac cggccagtat ccgctggatc
     6421 tcttgcgcca tcccggcgtg atttaggaaa tcccgataag cggccgtggt gacggcaaag
     6481 ccgggcggga cccggatgtt ggcgcgcagc atctcgccca ggctggcatt tttgccgcct
     6541 acggttggca ggcatgtcct atcgcagttc tgcagccaga tgatataagg ttcgtcggcc
     6601 atgggaagtt ggtgtgatgc gtgatcatgt gatcacgtgt cacgtgtcac gtatcacgca
     6661 tcacgttttc gtcacgtgcc tcccatcaac catgcaatca gttcaagacg gtgacgacgc
     6721 cggacgagcc atcgacgcgc agcatctggc cgttcttgaa ggtcttggtg ccgtagccgg
     6781 tgccgatgac cgccggcagg ccgtactcgc ggcagacgat ggcggcgtgc gacatcatgc
     6841 cgccgatgtc cgtgacggtc gccttgatgc gggtgaacac cggcgcccag ctcggcgtgg
     6901 tgatggggca gaccaagatt tcgcccggct gcacgtctga aagctcctcg acactggtga
     6961 tgacgcgggc cggtccctcg acgacgccgg gcgatccggc gaatccagtc agttctttcg
     7021 catcttccgg cttggccttc ggctgcagcc agcgctgaat gctgtcggtg gtgatgcccc
     7081 acagcatgat cgtgaacggc tcggtgacga cctccggcgg ctcgcccagt gcaggcggcg
     7141 gcgcccacgc cttgagcttc tcgaacagaa ccttgcgctc ctcgacgatg gacggccagt
     7201 atttggggcc gcgcgcttcg gtgccgaccg cccacgatgc gatcaggtcg tacagcgccg
     7261 cctggacctc gtagcgatgc aggtagaaga gatcgtctgt ctccttgaag aagccgtgct
     7321 tgacgaacag ccggccgaat tcccgcatct tgttccagaa gatggtgtga tgccagtgct
     7381 ccacgtagaa gttgtggttc tcgacgtagg ggaagacggt gcgcgccagg ccgagctgct
     7441 cctgaaacgc cttcttgtcc tcctcggtct ggagcagcgc cgtgtactcg gctgccagcc
     7501 ggtcgcgctc tttctggatc tcgcccagcg ggcgcgcgat gtcctggccg gcctcgatct
     7561 tctcgacgta gccacggatg gacaggcagg gcacggtcat atcgtcgatc caactgcgat
     7621 gcaaatggta aaagcccgtg ccggtggaga aatagaacca cggatctttc gacttctcta
     7681 gctcgtccag ccacttcttc ccggcatccg acttctgcat ttcggcgatg acttcgtccg
     7741 gctggacggt tttcttgaat acgccggcca ccccaaggtc gacggctgcc tgggccagcc
     7801 gcttcagctc ttcgtcaggc cggaagagga tcacgtcgat gccggaaacc agcttggcaa
     7861 ttgactggtc agaaatgtcg gggaaggctt tcttgcagaa gccgtagtac gtcaggtagg
     7921 ccgcgtagcc caggttgagg aattcgaagt ggaggtgcca gattttgtgc atgttttcga
     7981 tagcccggtt gtaggcttcg agcagatggt aggcgctggt gacgcccttg ccctccttga
     8041 ccaccgaaat gtcttccatc tccggcagat cgggcacctt gagaccctgc aagtcgcgga
     8101 tgaccgcttc ggccttgccc ttccattgct cgtacagctc gtcccagtgc tggtagtaat
     8161 agccggcgcg ctccatgaaa tgacccaccc gctcgccgat caccttttcg tcggtgagcg
     8221 cgttggcgct gatgtaaagg tagccgttca gcacgcgctg atcgataccc agcgccggcg
     8281 gcacgatgaa cacgcgcgag ttgtattggc cgagcgccac ccaccagctc tcgcaagtga
     8341 tcgtgtcgaa cggataaagc ggctccgggt ggtgcatgcc gtcctgaaac cagaacttgc
     8401 cttcttcaaa ttcgcgccgt tccgagctga acagatagta gtacgggtag agtttctccc
     8461 acccctcaca ccccggaggc gtgggaacct cgaatggacc gggaaatttc ccgtcagtgc
     8521 tggacatgtg tgcttcctcc ttcctctctt cggcatctgg agatgcctcg cacaaggccc
     8581 ggggctgatc cggtctccgc gtcgcatgtc cgggcgcggc ggcgcagagg cgcacaacgg
     8641 gtcacaggcg ctcgtcagtc tgatctcaga acagtcgcac ctccttgggc gaatagcaga
     8701 tagcagatgg cagatagcag atagcggatt gcagatagtc gcccacgctg tcatcagcca
     8761 tcagctacca gccatcggcc atcagccatt agccatcagc cacgagccat cggccatcgg
     8821 ccgtcgagcc agtcgggcaa acgattcacg tccggcagga cgagatcggg acgcagcccc
     8881 accggcgcag ccgctgcctc ttccgccgtc gtcgagccag tcaacaccag gatcgtgaac
     8941 agcccggcct gcttgcccat ggcgatgtct tgcttgagct ggtcgccggc cacggcgccc
     9001 cgctccggcg ggacgccgag catcttcata cccattgcgc cggcccactt cgacggcttg
     9061 cccgtgacca gcggctcttt gccggtggcg taggcgaccg ccttgaccat cggcccggtc
     9121 gccgggatga agccatccgc tgatggcacg cgagcgtcct cgttcgtcgc gatgaactcc
     9181 gcgcccttcc agatggcctg gcacacctga ttcaaggctt tctggttgaa ctccgggtct
     9241 ttgcccatca ccaccacctc agcctcggtc gcgtggtcga ggtcgatcag gttcaaacct
     9301 tgcgcctgca tcgcttcctt caagcccggc gcgcccacaa ccaggatctt tcggtccgcg
     9361 tgcagctcgc gcgtcacgct cgcggcaatc agtgccgagg tcatgatctc gccggcccgc
     9421 gccggaatgc ccatcgcatt cagacgctcg acccagaagc cgatcgtatt ctgattatcg
     9481 ttggtgagga aggtgaagcg gacgccgcgc cggcgcagct cggccagcgt gtccggcaca
     9541 cccggaatgg cattcttgtc cttcgccacg cagccgtcaa tgtcgaagat aatgccctct
     9601 atcggacgca tgcggtctcc atcatgcgtg aaacgtgata cgtgatgcgc gatgattggc
     9661 gtcacgcatc acgttctccg cagcggctag gcctgtgcca tctccataac ccgctgcggc
     9721 agatcggcgg gcatggcgaa atactcgtcc caatgctcct tgacgtgctt gaccatgttc
     9781 tcgtcgagga agatcggctt gggcttttcc ttccactcgt aaggcgtcgt gcagtcgatg
     9841 atgatgcgcg aagtcatgta gcgcgtgtcg ccgatcggca gcgaaggatc gagcggcgtc
     9901 gagcggccgc gccggatgat ctgcgtgccg aaatcgggct ggtacttgaa gctcagcgcg
     9961 tagatcacct gatccatgtt ttccgggtcg atgtcgtcgt cgacgagaat cacggtcttc
    10021 aggccgtagt tgccggtgac ggtggcgaag gcggccagcc cgacctgcgt cgagtgtccg
    10081 tggtacatcg gtttcatgga gatgatgacg agcatgcgcc cggcgctggc cggcgggcca
    10141 tacacagcct tgataccggg gatgttcatg gccttcagat cattccacag cgaggcagtg
    10201 cggttgacgg ccatgatcat gtgtgtatcc gtgatcggct tgccgacggt cgtcgaccac
    10261 aaaatggggt tgttgcggta ggtgatggcc ttgatctgga tgaactcctt gggaaattcc
    10321 tttccggagt aatggcctgt gtactcgccg aacggccctt cttgaagcgt ctcgccgggc
    10381 atgatctcgc cctcgatgac cagctcggcg gtggccggca ccgaaatgtc gttcgtcagc
    10441 gcttgggtga cttcgaccgg ctctccgcgc agcgccccgg cgatctcgta ctcgctctcg
    10501 ttcaagccga agagcgtgct tccgcacaag aacaggacgg gttccgcgcc gcacacgtag
    10561 gccaccggca tagggatgtt cttcgcggcg tacccgcgca ggtcgatttc cgagtctttg
    10621 gccttgatca tctgaatcga ggcgcgcttc tcatccacca ccatcaagcg gtaggttccg
    10681 acgttgagtc tgccggtgtc caggttgcgc gagatcaggt agcccgccgt gccgagatag
    10741 cggccgccat cgccggggta aaaccagggg gcgggcagct tgaaaatatt cacgtcgtca
    10801 ccctgcacga cgttctcgtg caccggcccg gagttcacga cgcggggcgg gacgcgcttg
    10861 ccctgaatgc ggtccaccca cgctcgcgac aggttcatca ggctcatgtc cgtgggcaat
    10921 tcaagcgcca aagcgaggcg ctctttggcg gtcagcgcgc tcgtcaagac gcgccagccg
    10981 ggatagtcct tgacgttctc gaataacaac gccttaccgc cgttgtgctc ctcttcgttg
    11041 accttggcga tatggctgag ctcgaggttc caatccacct ccttgtagat gcgccgaagg
    11101 tggtcttcgg cctcgagtcg gtccagaaac tcacgcaaat cacggtacgg catggtctta
    11161 tcctcctaat ggaagtgagt tttcagactt cggaagtctc aagacgtgtt ctcgccggcg
    11221 gcggtggccg gacatccgag tcatttcgct ggcgagatgc cacttaaaga cttccgaagt
    11281 ctcggccgta gctcatcgat ccgagtctca gacttcggaa gtctcaagac gtgttctcgc
    11341 cggcgacggt ggccggccat ccgagtcgtt tcgctggcga gatgccactt aaagacttcc
    11401 gaagtctcgg ctgtggctca aattgagatc tggcgagcct gctttctcgg gttcgccggc
    11461 ggacatgatt tggtacgcct tgtcgatatg ctccttcatc acctgcgcag ccgtctccgg
    11521 gtcgcgcgcg atgatcgcgg ccaggatgca ctcatggtca tgaagccggt cgtgagccgc
    11581 ctccggggtg atccgcgtcc ggcgtgccgc ctccaggtac aaatcgtgga tcgtgcgata
    11641 catgagcgcg agcacctgat tgtgagcggc gtcggcgatc gccagatgga aattcacgcc
    11701 ggcccgcacg ttcgtctcga cgttcgtgat gctcgcccgg gagcgctcca gcgccgcacg
    11761 caacgcctcg atgtcttcct ccgtcgcccg ttcggccgcc agttgcgcga gcagcacttc
    11821 catgaactgc cgcgcctcca tcacttccga gatgatgccc atctgagaaa tgcgggacca
    11881 gtccaccatc aagggcaaga tgcccggctc gcggacaaaa gtgcctttcc cctggatcac
    11941 ttccacgacg cccacgagct gcagcacccg catcgcttcc cggatcgagg agcggcccac
    12001 cccgaactgc tccgccaggt ccatctcgga gggaagatgg tcgccaggct ggagcttgcc
    12061 ttcggcaatg agcttctgaa cttgctcgag cacgacttcg gggatagagc gtgacttctg
    12121 aatggtctgg aagtcggcca tattttcagc aataactcca tcagaaacct ggtttctcgt
    12181 agaaaccagg tttatctcta gccaagcaag ttgtcagatg tctgatgacc tgataacatc
    12241 actatagcat gaatttccta tttgtaaata tacataactg ggaaattcaa gttttttaag
    12301 gttacagata cacctggccg gtcttatcca ccggtgttgg ctgtagactc gctccgcgtc
    12361 gtgaagacct gtcaggtcta cccggagaat ccagttgcgt ttgggatgtg agggagtgac
    12421 acctcgtccc ggtgacccag gccgagcgag ttgtcgatga aatagaggtg agccggcacg
    12481 accctgtacc agacgattcg gctcatggcc ttcacaatct cggccggggc ctgcgccagg
    12541 ttgccgacga tggggaattt cgagccgtag cgtgccatcg ccgccgcttg ctctgcgcct
    12601 tcgactctcc tcgcctcccc ttccagttgg atgcccttga tctcgcgcca gtcgcggtaa
    12661 tcttcctgga tggttgccga gacgatgggt tgggcctcga cgttgaggct gtggcggctc
    12721 gtcggcgcgg agaggaaata gagggtgaac ccgtcgttga cgtagaagac ggccgcagcc
    12781 cacaggccct gcgggccggc ggttgccagc gtcatcacgt tgtgctcgcg caggtaggcc
    12841 agggtctttt cgtgtagcga ggagtggtct tcggtcatca gcctagccaa actctaagag
    12901 actgtctgaa aactaaccat caagtcacca agacacgaag aaaaattgga gattcttggt
    12961 gccttcgtgt ctttgtggta agtttggggg ctttgcagac aggctctaaa caggaccctc
    13021 gataacttga taatgcgccg agtggctatc gaacctggac aagagctgcc tggccttcgg
    13081 tggcacaacc gccacggtat agtcc
//
LOCUS       DBNHXB010000265         4737 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1410144, whole genome shotgun sequence.
ACCESSION   DBNHXB010000265 DBNHXB010000000
VERSION     DBNHXB010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1410144"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1713
                     /locus_tag="AC162G_10840"
     CDS             <1..1713
                     /locus_tag="AC162G_10840"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5076358.1"
                     /translation="HAELALAFASHAAIAIENARLFETEQRRAEQFRAINEVGRHITS
                     ILTVDELLAQTARLIQETFRYYFTSIGLIEGDEVVVKYGDPLRLRVGQEGIIGWVAGS
                     GEPLLVPDVSQDSRYVATTSASQTRSELAVPIKSRGRVIGVLDVESDRVNAFDESDVA
                     VVQSLANQLGVAIENARLYESEQRRAEQFRVLAEVGRRITSILDVDQLLSQTVQLMSQ
                     AFGYYHVGIAMIEGDMAIYRTGAGTLWNEPPLQFQPPRLKVGQEGITGWVAGSGEPLL
                     VPDVSQDSRYVWMEGSQTRSELALPILAKDRTIGVLDVQSDRVNAFDQSDLVVLQSLA
                     HQLAIAIENARLYEQAQQLAALEERQKLARELHDSVSQALYGIALGAKTARTLLDRDP
                     IQVAQPLDYVLSLAEAGLAEMRALIFELRPESLEMEGLVAALTKQTASLHARYHIEVQ
                     TAFGDEPRLPLPTKEALYRIAQEALNNIVKHARASRVDVRLEPADGSVGLEIGDNGVG
                     FDPQGEFPGHLGLRSMRERAEKAGGTLAVESAPGQGTCIRVRVPVNRNSGGDAPSPPR
                     ADSR"
     gene            complement(1746..1976)
                     /locus_tag="AC162G_10845"
     CDS             complement(1746..1976)
                     /locus_tag="AC162G_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076359.1"
                     /translation="MFNNYAGSVPIAGRRTGLKNSINIRPRGVSMERREWIAAVSITV
                     LIVALVALIGLAALSQIDTADFVRLDVVSRGW"
     gene            2331..3644
                     /locus_tag="AC162G_10850"
     CDS             2331..3644
                     /locus_tag="AC162G_10850"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5076360.1"
                     /translation="MRNNQLLYLSLCCFIILFVGMGLFPVLPSYDAQFGATRTVVGIH
                     FALMYAANVAGHMLAGRLAARLTRQGAFVMAGMLGIPALILLGHATALWHVIALTAIT
                     WFCGGAGFALVGVFTGLHADRDSRGRSFVVTYLAYPLGATFGGATVGRLVAWQGYPVM
                     FAVLGAVWAFLPLIGLLKLKDKQTSKPAASTAQAGGASTPSSRTPALAHTPPSRASAR
                     ALHWLLTVMLMSATAIGVGRLGMPLSMQALDFPAGAIASVTAMSGLVAVPVALLIGAL
                     SDRLGRRRFLALIYVLVASGMLILSAADQVWHFWLAATLMFVALSTNGALTSALATDI
                     LPQEALGRSLPRITATNSAAAVLSFASTGFVMDTFGATALYVAAAGLALIAALQLRRV
                     ESHAQRVEARVAYLGQYNKPTVQSAIPLNTQLPDAHQLRRMESSC"
     gene            3638..>4737
                     /locus_tag="AC162G_10855"
     CDS             3638..>4737
                     /locus_tag="AC162G_10855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5076361.1"
                     /translation="MLSQREISHDSTMRLSDALGAHAVVIGGSIAGFTAARVLTNHFE
                     RVTIVERDRLPDTPDFRPGAPQARHVHVLRLRGQMILEQQFPGLIDELLAAGAVTVNA
                     GNEAEFFLFGHWRAPRYRSAIVSVAGSRPLLEYTIARQLAARPGVAILQEHDVVGLNV
                     DERGERVIGVRLRQRGEAHAGGSELAADLVVDASGRASKAQQWLAGLGYAPPRETVVD
                     AFPGYTTRLYRRPAEFRSGWKTMYIIPTPPHSPRGGVIVPLEGDRWMVTLIGMGRDYP
                     PTDEEGFMAFARSLPSSRLHDAIKGAEPLTKPYAYRNNANRLRHYDQLPRYLEGFLVS
                     GDSVLTLNPTHAQGMTAAALGSLALQRSLQAHR"
BASE COUNT          839 a         1475 c         1546 g          877 t
ORIGIN      
        1 cacgcggaat tggccctggc attcgccagc catgcggcga tcgccattga gaacgcgcgc
       61 ctgtttgaga ccgagcagcg ccgcgccgag cagttccggg ccattaatga ggtagggcgt
      121 cacatcacct cgattctgac ggtggatgag ctactggctc agacagcccg actgattcaa
      181 gagactttcc ggtattactt cacgagcatc ggactgatcg aaggggacga ggtggtcgtc
      241 aaatacggcg atcccctgcg cctgcgggtg gggcaggagg gcattatcgg ctgggtcgcc
      301 gggagcggtg aaccgctcct ggtgccggac gtgagccagg attcccgata tgttgctacc
      361 acgtcggcca gccagacccg atcggagttg gcggtgccga tcaaatccag aggccgggtc
      421 attggcgtgc tggacgtgga gagcgatcgc gtcaacgcct tcgacgaaag cgatgtggcc
      481 gtcgtgcaat cgctggccaa tcagctcggc gtggcgatcg agaatgcccg tctctacgaa
      541 tctgagcagc gccgcgccga gcagttccga gttctcgccg aagtcggccg ccgtatcacg
      601 tccattctgg atgtcgatca gcttctatca caaaccgtcc agctcatgag ccaggccttc
      661 ggttactatc atgtcggcat tgccatgata gaaggtgaca tggcgatcta tagaaccgga
      721 gcgggcacct tgtggaatga gcctccgctt cagttccagc caccaaggtt gaaagtgggg
      781 caggaaggga tcacgggctg ggttgcaggc agtggcgagc cgctgctggt gccggacgtg
      841 agccaggact cccgctacgt ctggatggaa ggcagccaga cgcgatccga actggcgctc
      901 cccatcctgg ctaaagatcg cacgatcggc gtgctggatg ttcagagtga ccgggtgaat
      961 gccttcgatc agagcgatct ggttgtgctt cagtccctgg cgcaccaact ggcgatcgcc
     1021 atcgagaacg cccggctgta cgagcaggct cagcaattgg ccgcgctgga ggagcggcag
     1081 aagctggcac gcgagctgca cgactcggtg tcgcaggcgc tgtatggcat cgcactcggc
     1141 gcgaagacgg cccgcacgct gctcgatcgc gatcccatcc aggtggcgca accgctcgac
     1201 tacgtgctgt cgctggccga agcgggcctg gccgagatgc gtgcgctgat ctttgagctg
     1261 cgccctgaat cgctggagat ggaaggattg gtcgccgcgc tgaccaagca gaccgcctcg
     1321 ctgcacgccc gataccacat cgaggtgcag acggcgttcg gcgacgagcc tcggctgccg
     1381 ctgcccacga aggaagcgct gtatcgcatc gcgcaggagg cgctgaacaa catcgtcaag
     1441 cacgcgcgcg ccagccgggt ggatgttcgg ttggagccgg cggatggcag cgtgggatta
     1501 gagattggag ataatggcgt gggcttcgat ccgcagggcg aattccccgg ccacctgggc
     1561 ctgcgctcga tgcgcgagcg cgccgagaag gcgggcggca cgctggccgt ggaaagcgct
     1621 cccggtcagg gcacgtgcat tcgcgttcgg gttcccgtca accgaaacag cggcggtgat
     1681 gccccgtcac cgccgcgggc cgacagcaga taagaggaac atcagcgggc cggacagagg
     1741 ttctgctacc agccacgcga tacgacgtcc aggcgaacga agtcagccgt atcaatttgg
     1801 ctgagcgcgg caaggccgat cagagcgact agcgccacga tcaacacagt gatactcacg
     1861 gctgcgatcc actcccgtcg ttccatcgag accccgcgag gtctgatatt gatggaattt
     1921 ttcagaccag tccggcgtcc ggcgatcggc acgcttccgg cgtagttgtt gaacatttcc
     1981 ccctcctgta tctgtatctc agagactgac ttaaaacctc gtggttgacg catccccata
     2041 cgtcaacagc gggcatctgt gcgcattgta tgctatgcgc gcctgcccgt catggctcaa
     2101 atgctggaag gggcattcct tccttgggcg catgacggcc tcggccaaag gatgtagtca
     2161 gaccctattc gcacgcaaga acccggacga aagtaaggga tatgaagctg acgatgagca
     2221 ttctcgggcc aatgcatccg aatgcccggc cgctcatccg actttcggcg tacatgtaga
     2281 aggtccgttt agtcgaatac agacgcagcg cgaatcagta gaatcctact atgcgaaaca
     2341 atcaactact ctacctgtct ttgtgctgct ttatcattct cttcgtcggc atggggctgt
     2401 ttccggtgct gccatcgtac gacgcgcagt tcggcgcgac gcgcaccgtg gtgggcatcc
     2461 acttcgcttt gatgtatgcg gcgaatgtag ccggccacat gctggccggc cggctggcgg
     2521 cacggctcac gcgacaaggc gcgttcgtca tggccgggat gctggggatt cccgcattga
     2581 tcttgctggg acacgctaca gccctgtggc acgtgatcgc gctgacggct atcacctggt
     2641 tttgcggcgg tgccggcttc gcactggtcg gcgtattcac cggtctgcac gcggatcggg
     2701 acagccgcgg caggtcattc gtcgtaacct acctggcgta tccgcttggg gccacgttcg
     2761 gcggcgcgac ggtgggccgt ctcgtggcgt ggcagggcta tcccgtgatg ttcgccgtgc
     2821 tgggtgcggt ctgggccttt ctgccgctca tcggcctgct gaagctgaag gataagcaga
     2881 cgtccaagcc ggcagcctcg acggcgcagg cgggcggcgc atcaacgcca tcaagccgaa
     2941 cacctgcgct cgcgcacacc ccgccgagca gggcgagtgc gcgggcgttg cactggctgt
     3001 tgactgtcat gctgatgtcg gctacggcta tcggcgtcgg gcggctgggc atgccgctgt
     3061 ccatgcaggc gctcgatttc cctgccggcg caatcgccag cgtgaccgcg atgagtgggc
     3121 tggtggccgt gccggtcgcg cttctgatcg gcgccctgtc cgaccgcctg ggacgccggc
     3181 gcttcctggc gctgatctac gtgctcgtcg caagcgggat gctgatcctg agcgcggccg
     3241 accaggtgtg gcacttctgg ctggcggcca cgctgatgtt cgtggcgttg agcacgaacg
     3301 gggcgcttac ttcggctctg gcgacggaca ttctgccgca agaggcgctc ggccgcagtt
     3361 tgccccgcat cacggcgacg aactcggcgg cggctgttct gagcttcgcg agcaccgggt
     3421 tcgtgatgga cacctttgga gcgaccgccc tgtacgtcgc agccgccggt ctggcgctca
     3481 tcgccgcgct gcagctaagg cgagtggaga gccacgcgca gcgcgtcgag gcaagggtcg
     3541 cctacttggg ccagtataac aagcctaccg tgcagtcagc cataccgctt aacactcagt
     3601 tgccggatgc tcatcaactc aggaggatgg agtcatcatg ctaagccaac gggagatttc
     3661 tcacgactcg acaatgcgcc tgtccgatgc cctgggcgcc cacgccgtcg tcatcggcgg
     3721 cagcatcgcc ggtttcacgg cggcccgcgt tctgaccaac cacttcgagc gggtcacgat
     3781 tgtcgaacgc gatcggctgc ccgacacccc agacttccgt ccgggcgcgc cgcaggcgcg
     3841 acacgtgcac gtcttgcggc tgcgcggtca gatgatcctg gagcagcaat ttccgggcct
     3901 gatcgatgaa ttgctggccg ccggcgcggt cacggtcaac gccggcaacg aagccgagtt
     3961 ctttctcttc ggccactggc gcgccccgcg ctaccgttcc gccatcgtgt ctgtcgccgg
     4021 cagccggccg ctgctggaat acaccatcgc acggcagctc gcggcccgcc ccggcgtcgc
     4081 catcctgcag gagcacgatg tggtgggact gaatgttgac gagcgcggtg aacgggtgat
     4141 cggggttcgg cttcgccagc ggggcgaagc gcatgcgggc ggatcagaac tggcagccga
     4201 tttagtcgtg gacgccagcg ggcgcgcgtc gaaggcgcaa caatggttag ccggcctggg
     4261 ttatgcgccg ccgcgcgaaa cggtggtcga tgcctttccc ggttacacga cgcgcctgta
     4321 caggcgaccg gccgaattcc gcagcggatg gaagacgatg tatatcatcc ctactccgcc
     4381 gcacagcccg cgcggcggcg tcattgtccc cctggagggt gaccgctgga tggtcacgct
     4441 gatcggaatg ggccgcgact atcctcccac cgacgaggag ggcttcatgg cgttcgcccg
     4501 cagcctgccc agctcgcgcc tgcacgacgc catcaagggg gccgagccgc tgaccaaacc
     4561 gtacgcttat cgcaacaatg caaatcgtct gcgccactat gatcaactgc cgcgttatct
     4621 ggaagggttc ctggtgtccg gcgatagcgt cttgacgttg aacccgaccc acgcgcaggg
     4681 gatgaccgcc gcggccctgg gtagcctggc cctgcagcgc agtctgcagg cgcaccg
//
LOCUS       DBNHXB010000266         3145 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4094089, whole genome shotgun sequence.
ACCESSION   DBNHXB010000266 DBNHXB010000000
VERSION     DBNHXB010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4094089"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /gene="mntA"
                     /locus_tag="AC162G_10860"
     CDS             complement(<1..373)
                     /gene="mntA"
                     /locus_tag="AC162G_10860"
                     /inference="COORDINATES: protein motif:HMM:NF047752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII toxin-antitoxin system MntA family
                     adenylyltransferase antitoxin"
                     /protein_id="HIR5076362.1"
                     /translation="MHFDEAKLKKVARRYNLDLIVLFGSHAKGRARKGSDVDVAVRAR
                     KRRWGDADWELGLVSDLTAALESAGEVDVAFLNGADPILLFEVASDGVPLFEAEPAGF
                     AQFRSYAARVYDDNRKWRERRR"
     gene            complement(538..1875)
                     /locus_tag="AC162G_10865"
     CDS             complement(538..1875)
                     /locus_tag="AC162G_10865"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR5076363.1"
                     /translation="MGTNVVMPQLGESVVEGKISKWLKKPGDAVKLYEPILEVETDKV
                     TTEVTAAGEGTLLKLYFDEGEVVQAETLIAFIGQPGETPPAGGKVDLHAAEPAHAAAA
                     ASVAPVAKPPAVTRVSPVVARIAAEHNVDVSLVPGTGEGGRVTKKDILAYVERGGTAK
                     PVSPLPVGEERVRELAPWEQPGLGELFRPTEEVFGAAKPAPAPAPMPTPVPAPAPMPG
                     EVVSLSSMRRSIAEHMVRSKHASPHVTTVFEIDMSRVVAHRNANKAAFERDGTNLTYT
                     PYFVAASVAALKQHPMANSSWREDGILLHRTVNVGVAVSLGDEGLIVPVIKNADSMNL
                     LGLARAVNDLADRARRKALKPDDVQGGTFTITNHGVSGSLFATPIINQPQCGILGVGM
                     VQKRAVVVTQDGVDSIAIKPMCYISFTFDHRILDGALADYFAASLKLALENWT"
     gene            complement(2040..3023)
                     /locus_tag="AC162G_10870"
     CDS             complement(2040..3023)
                     /locus_tag="AC162G_10870"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR5076364.1"
                     /translation="MAEKTLIEGIREALDEELERDPAVFITGEDVGPRGGVFRATMGL
                     FKKYGPSRIIDSPLAESSIVAIGVGAAMNGMRPVCEIQFADFIHPAYNQIVNEAARMY
                     YRTGGSTTVPMVIRTPYGGGIGGGLYHSQSIEATFAHVPGLKVAVPSNPHDAKGLLKS
                     AIRDNNPVLFLEPKKGYRLIKGEIPDGEYTVPLGAAKVTREGADITVYAYGIMHFYCQ
                     QAADEVAKEGIGVELVDLRTLNPLDKETVLNSFKKTGKALIVYEDNRFLGYGAEIAAI
                     LADEAFHYMDAPIMRLAGPDVPGVPYSHKLQDWFMPNPQKIAEAMRKLAAY"
BASE COUNT          518 a         1053 c          970 g          604 t
ORIGIN      
        1 ggcggcgacg ttcgcgccac ttgcgattat cgtcgtagac gcgtgcggcg taggatcgga
       61 actgggcgaa gccggccggc tccgcctcga acagcggcac gccgtcgctg gcgacctcga
      121 acagcaagat cggatcggcc ccgttcagga acgcgacatc gacctcgcca gcggattcca
      181 atgcggcagt caggtcactc accaggccga gttcccaatc ggcatcgccc cagcgccgct
      241 tgcgagcacg caccgccacg tcgacatcgc tgcctttgcg ggcgcgtccc ttcgcgtgtg
      301 aaccaaacag cacgatcagg tccagattgt agcggcgcgc gaccttcttg agtttcgctt
      361 cgtcgaagtg cacgggcaat tcggattctc cattcagtac cgcagtgact ccacgtgtca
      421 tcggcgaaca agatttccag gacctgcttt atcgcggaac tcgcgctccg ccccgataag
      481 cccaccggcg gcaaccgcca gcagcagcct gttcagatcc tcgagcggaa acgtcatcta
      541 cgtccaattc tctagcgcca gcttgagcga cgctgcgaag tagtccgcca gggcgccgtc
      601 caggatgcga tgatcgaatg tgaacgagat gtagcacatc ggcttgatcg cgatcgaatc
      661 gacgccgtcc tgcgtgacga ccacggcgcg cttctggacc atgccaacgc cgagaatgcc
      721 gcactgcggc tggttgatga tcggcgtggc gaacaggcta cccgacacgc cgtggttggt
      781 gatggtgaac gtgccaccct gcacgtcgtc gggcttgagc gccttgcgcc gcgcgcggtc
      841 ggccagatcg ttcaccgcgc gagcgagtcc cagcaggttc atcgaatcgg cgttcttgat
      901 taccggcacg atcaggccct cgtcgcccag cgacacggcg acgccgacgt tcaccgtccg
      961 gtggagaagg ataccgtcct cgcgccacga cgagttggcc atcggatgct gcttcagcgc
     1021 ggcgacggac gctgcgacga agtacggcgt gtaagtgagg ttcgtgccgt cgcgctcgaa
     1081 cgcggccttg ttcgcgttac ggtgcgccac gacgcgcgac atgtcgattt cgaagactgt
     1141 cgtcacgtgc gggctggcat gcttggagcg caccatatgc tcggcgatcg agcggcgcat
     1201 cgacgagagc gagacgactt cgccgggcat gggcgcgggc gccggaacgg gcgtcggcat
     1261 cggggcaggc gccggcgcag gcttcgcggc gccgaacacc tcctcggtcg gacggaacag
     1321 ctcgcccagg ccgggctgct cccacggcgc aagctccctc acccgctcct ctcccacagg
     1381 gagaggggag acgggcttcg ccgtgcctcc gcgctcgacg tacgccagaa tgtccttctt
     1441 cgtgacgcgc ccgccctcgc cggtgccagg cacgagcgac acgtcgacgt tgtgctcggc
     1501 ggcgatgcgc gccacgacgg gcgatacgcg cgtcacggcc ggcggtttgg ccaccggggc
     1561 gacgctcgcc gctgccgccg cgtgcgccgg ctcggccgcg tgcaagtcaa ctttgccgcc
     1621 ggccggcggc gtctcgcccg gctgaccgat gaacgcgatc agcgtctcgg cctgcacgac
     1681 ctcgccctcg tcgaaataca gcttcaggag cgtgccctcg cccgcggcag tcacctcagt
     1741 cgtcaccttg tcggtttcga cttcgaggat cggctcgtac agcttcacgg cgtcgccggg
     1801 cttcttgagc cacttgctga tcttgccctc gacgacgctc tcgccgagct ggggcatcac
     1861 gacgtttgtt cccatgatcg gtctctccaa tgggcggatg gcagatagcc gatggcagat
     1921 ggcagatggc ttatcgcatg tcgcgtatcg cgtatcgcgc gtcgcagcgt gtcacctgtc
     1981 acacttcacg cctcacgttt tacgtatcac gtttcacgca tcacgtatca cgtcacgtat
     2041 cagtaagccg ccagtttcct catcgcctcc gcaatcttct gcgggttcgg catgaaccag
     2101 tcctgcagct tgtggctgta cggcacgccg ggcacgtcgg ggccggccag ccgcatgatc
     2161 ggcgcgtcca tgtaatggaa cgcctcgtcg gccaggatcg ccgcgatctc tgcgccgtag
     2221 cccaggaacc ggttgtcctc gtacacgatc agcgccttgc ccgtcttctt gaacgagttg
     2281 agcaccgttt ccttgtcgag cgggttgagc gtgcgcagat cgaccagctc gacgccgatg
     2341 ccctccttcg ccacttcgtc ggcggcctgc tggcagtaga agtgcatgat gccgtaggcg
     2401 tacacggtga tgtccgcgcc ttcgcgcgta accttcgccg cgccgagcgg cacggtgtac
     2461 tcgccgtccg ggatttctcc cttgatgagc cggtaccctt tcttcggttc gaggaatagc
     2521 accgggttgt tgtcgcgaat tgccgacttg agcaggccct tggcgtcgtg ggggttcgac
     2581 ggcacggcga ccttcaggcc gggcacgtgc gcgaatgtcg cctcgatcga ctgcgagtga
     2641 tacagcccgc cgccgatgcc accaccgtac ggcgtgcgga tcaccatcgg cacggtcgtt
     2701 gagccgccgg tgcggtagta catgcgcgcc gcttcgttga cgatctggtt gtaagccgga
     2761 tggatgaagt cggcgaactg gatttcgcac accgggcgca tgccgttcat cgccgcgccg
     2821 acgccaatgg cgacgatcga cgattcggcc agcggggaat cgatgatgcg actcggcccg
     2881 tacttcttga acaggcccat cgtcgcgcgg aacacgccac cgcgcgggcc gacgtcctcg
     2941 cccgtgatga acaccgccgg atcgcgttcc agttcctcgt cgagcgcctc tcgaatccct
     3001 tctatcagag ttttctccgc catcgattct cctcactcgt ccaccaatta ctaatgacca
     3061 attaccgatt ggagtttaga ctaaccgccg ctttgagggc cttttcgcca catgtgccgc
     3121 catatttggc ggttttgtaa catgt
//
LOCUS       DBNHXB010000267         5520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_403856, whole genome shotgun sequence.
ACCESSION   DBNHXB010000267 DBNHXB010000000
VERSION     DBNHXB010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_403856"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="AC162G_10875"
     CDS             <1..473
                     /locus_tag="AC162G_10875"
                     /inference="COORDINATES: protein motif:HMM:NF016176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR5076365.1"
                     /translation="NRLQTAIGRTKGVAGDVLVDFAQPSNSQIGEIVIDVSQFTSDET
                     RRDNYIRRDGLQSSQYPYARFVPHSIEGLPDQVVPGEDLTFTIAGDLTVKETTLPVTW
                     IVTLRVEDDRLVGSAASEILMSDFGVGPIQVATLVTEDLVKLHFDFVAVPVEGS"
     gene            470..1195
                     /locus_tag="AC162G_10880"
     CDS             470..1195
                     /locus_tag="AC162G_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016906542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase family protein"
                     /protein_id="HIR5076366.1"
                     /translation="MIHQRTARPHVILFFVIACFVAGCASQPQATATPTHESVAVVPA
                     DAQAPPASATPTVAVPTPAPAMTPTPTQAQAILPTPACSADTGATPAMTEGPYYTPNA
                     PERTSLLEPGMTGTRIVISGYVLSTDCQPIPGALVDFWQADADGVYDNAGYRLRGRQY
                     TDANGRYTLETVVPGLYPGRTRHIHVKVQAPGGPILTTQLFFPGEAQNDTDTIFDPGL
                     LLSPVQETGNDLTATFDFVVEQN"
     gene            1270..2358
                     /locus_tag="AC162G_10885"
     CDS             1270..2358
                     /locus_tag="AC162G_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vgb family protein"
                     /protein_id="HIR5076367.1"
                     /translation="MIMTLVLAACATQPQPSVAPTNTARPASTPTESARPPDAPAATT
                     APSPELPTAEPTEHAPGTPAPPTIQEYPVPPGSHPHDVAPAPDGTVWYTAQRSGELGR
                     LDPATGETRHIPLGPGSAPHGVIVGPDGAAWITDSGLNAIVRVDPATEDVQVFPLPAG
                     RGNANLNTAAFDGDGVLWFTGQNGVYGRLDPAVGQVEVFDAPRGRGPYGITSTPDGVV
                     YYASLAGSHIAGIDAATKLASVIEPPTPGQGARRVWSDSQGRLWVSEWNAGQLGMHDP
                     ATGEWREWRLPGDNPQPYAVYVDDRNLVWLSDFGANAMVRFDPATETFEVFTLPSSPA
                     SVRQILGRPGEVWGAESGVDKIVVLRIP"
     gene            2450..3580
                     /locus_tag="AC162G_10890"
     CDS             2450..3580
                     /locus_tag="AC162G_10890"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013555821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5076368.1"
                     /translation="MKAGKSSRLWIIVAALTFLAAACGTGPQPSSAEEVVRIAVAASA
                     TPLPSATPTPEPTPTSTPTATLTPTPHPLTIPSMREREYPGSEVTIVETLAPGSNYGR
                     YIASYESEGLTIYALLTVPNGERPAAGWPVIVFNHGFIPPDVYRTTERYVAYVDWLAR
                     SGYIVFRSDYRGHDNSEGEARGAYGAPDYTVDVLNAVATMKRFPDADPNRIGMWGHSL
                     GGYLTLRSMIITGDIKAGVIWAGVVGPYPNLFVRPTATPTPEGWTPEPTTTATPRFGR
                     RWRTELVELYGSPEENPDFWNSISSNSYLSDVSGPIQLHHGTRDASVPLAASETLYRQ
                     MLEAGQPVELYTYEGDDHNLSQSFGLAMQRTIEFFNRYLKGP"
     gene            3646..4761
                     /locus_tag="AC162G_10895"
     CDS             3646..4761
                     /locus_tag="AC162G_10895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014686340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5076369.1"
                     /translation="MKAGTFPHRWIVVIGLALLMAACGSNPQPPQTTRVAEVPPTASR
                     TAAPSPTDTPTPTHTPTATPTSTPTPHPLTIVSMRQREYPGSDITIEETLQPGGNYSR
                     YIASYESEGLTIYALLTVPNGERPATGWPVIVFNHGFIPPDVYRTTERYVAHVDWLAR
                     SGYIVLRPDYRGHDNSEGEARGAYGSPDYVVDVLNAVAAMKRYPNADPNRIGMWGHSM
                     GGYITLRSMVIDGDVKAGVIWAGVVGSYPDMINRWRPTPTPGPTTTATPTGRRRWRTE
                     LIATYGSPEENPEFWSSISANSYLGDLSGPIQLHHGTADRSVPIGFSETLYQEILAAG
                     KPAELYTYQGADHNIANSFGLAMQRTIEFFDKYVKGS"
     gene            4927..>5520
                     /locus_tag="AC162G_10900"
     CDS             4927..>5520
                     /locus_tag="AC162G_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR5076370.1"
                     /translation="MVEAFLQLSPVAQAILATGFTWGMTALGAGMVFLSHSVNQKMLD
                     WMLGFAAGVMIAASYFSLLAPAIAAAEGGPLPAWFPAATGFLAGAIVLRLIDRILPHL
                     HPGLRIDQAEGVKTTWQRSVLLVLAITLHNFPEGLAIGVAFGAAAAGMPSATVASAIA
                     LAIGIGIQNFPEGTAVAMPLRREGVSRWRSFLYGQASA"
BASE COUNT         1029 a         1790 c         1755 g          946 t
ORIGIN      
        1 acaaccgact ccagaccgca atcgggcgga cgaaaggcgt cgccggcgat gtgctggtcg
       61 atttcgcgca gccgtccaac agccagatcg gcgaaatcgt catcgacgtg agtcagttca
      121 cgtcggatga aacgcgccgc gacaactaca tccgccgcga tggcttgcaa tcgtcgcagt
      181 atccctacgc gcgcttcgtc ccgcactcga tcgaaggatt gcccgatcag gtggtgccgg
      241 gcgaggacct caccttcacc atcgctggcg atttgactgt caaggagacg acgctgccgg
      301 tcacgtggat cgtgacgttg cgcgtggaag acgatcggct ggtcggctcg gcggcgagcg
      361 agatcctgat gtcggacttt ggcgtagggc caatccaggt agcgacgctg gtgacagaag
      421 acctggtcaa gctacacttt gacttcgttg ctgtgccagt ggagggatca tgattcatca
      481 gagaaccgca cggccccatg tgatcctgtt cttcgtgatc gcatgcttcg tcgcgggttg
      541 cgccagccag ccgcaggcaa ctgctacgcc aacgcacgaa tctgtagcag tcgtaccggc
      601 agatgcacaa gcgccgccgg catcggccac gccaacagtc gcggtgccga ccccagcgcc
      661 tgcgatgacg cccacgccaa cgcaggctca agccattctg ccgactccgg cctgtagcgc
      721 tgacacgggc gcgactccgg cgatgaccga agggccttat tacacgccca atgcgccgga
      781 gcgcacgtca ttgctggagc cgggcatgac cggcacgcgc atcgtcatca gcggatacgt
      841 cctctcgaca gattgccagc cgattccagg cgcgctcgtc gacttctggc aggccgacgc
      901 cgacggagtc tacgacaacg ccggctaccg gctgcgtggc cgccagtaca cggatgccaa
      961 cgggcgttac acgctcgaga cggtcgtgcc cggattgtat cccggtcgca cgcggcacat
     1021 tcacgtcaaa gtgcaggcgc ccggcggccc gatcctgacg actcagttat tctttcctgg
     1081 cgaagcgcag aacgacaccg acacgatctt cgacccgggt ctactgctct cgccggttca
     1141 ggaaacaggc aacgatctga cggccacatt cgattttgtt gtggagcaga actgagagcg
     1201 accatgcgat cgggcattga tccatccgaa cggatgcgag gtgcgaatat gcgccggatc
     1261 agcgcaagca tgataatgac attggtgctg gccgcatgtg cgacgcagcc gcagccatcc
     1321 gttgcgccga caaacacagc gcggcctgct tcaacgccga ccgaatcggc tcgcccgccg
     1381 gatgcgccag cggcgacgac tgcgccttcg cctgaactgc cgacggccga gccaaccgag
     1441 cacgcgcccg gcacaccggc cccaccgacg attcaggaat atcctgtgcc gccaggctcg
     1501 cacccacacg acgtcgcgcc cgcgcctgac gggacggtgt ggtacactgc gcaaagatcg
     1561 ggtgagctgg gccggttgga tccggccacc ggcgagacgc gccacatccc gcttgggcca
     1621 gggtcggcgc cgcacggcgt gatcgtcggg ccagatggcg cggcgtggat cacggacagt
     1681 ggcctcaacg cgatcgtccg tgtcgatccg gcgacggaag atgtgcaggt gtttccttta
     1741 cccgccgggc gcgggaacgc caacctgaac acggctgcat tcgatggtga tggcgtgcta
     1801 tggttcaccg gccagaacgg agtgtacggc cggctcgatc cagcagtcgg acaggtcgag
     1861 gtgttcgatg cgccgcgcgg gcgcgggccg tatggtatca cgagcacgcc ggacggcgtt
     1921 gtgtactatg cgtcgctggc gggcagccac atcgccggca tcgacgcggc gacgaaactc
     1981 gcatccgtca tcgagccgcc gactccagga cagggcgcgc gccgcgtgtg gtcggactcg
     2041 cagggccgat tgtgggtgag cgaatggaac gcaggccagc tcggcatgca cgatccggcg
     2101 acaggtgagt ggcgcgagtg gcgcctgccg ggcgataatc cccagccgta tgccgtgtac
     2161 gtggacgacc gcaacctcgt ctggctgagc gacttcggcg cgaacgcgat ggtgcgattc
     2221 gacccggcga ccgagacgtt cgaggtcttc acactgccca gctcgcccgc cagcgttcgc
     2281 cagatactgg gtcggcctgg ggaagtgtgg ggcgccgagt cgggcgtgga taagatcgtc
     2341 gtgctgcgaa ttccataggt tgggacggca gttgcactgc cgtcccaggc cgccgcagtg
     2401 caactgcggc gggaactgca gaacacagac taaagaagga gttgactcga tgaaggcagg
     2461 gaagtcttca cgactctgga tcatcgtagc agcactgaca ttcctggcgg ccgcctgtgg
     2521 aaccggcccg cagccgtcgt ccgcggagga ggttgtccgc atcgccgtgg cggcctcggc
     2581 cacacctctg ccttctgcca cgcccactcc tgagccgacg cccacgtcga cacccacggc
     2641 gacgctgacc cccacgcccc acccgctcac gatcccatcc atgcgagaac gcgagtatcc
     2701 tggcagcgag gtcaccattg tcgagacact agcgccaggc agcaattacg gccgctacat
     2761 cgcgtcctac gagtccgaag ggctgaccat ctacgcgctg ctgacggtgc ccaacggcga
     2821 gaggccggcg gccggctggc cggtcatcgt cttcaatcac ggcttcatcc cgcccgatgt
     2881 gtatcgcacg accgagcgct acgtggcgta cgtggactgg ctggcgcgca gcggctacat
     2941 cgtctttcga tccgactatc gcgggcacga caactccgag ggcgaggcgc gcggcgcgta
     3001 cggcgcgccg gattacaccg tcgacgtgct caacgccgtc gccacgatga agcgctttcc
     3061 ggacgccgat ccgaatcgca ttggcatgtg gggccattcg ctgggtggat acctgaccct
     3121 ccgctctatg atcatcaccg gcgatatcaa ggccggtgtg atctgggccg gcgtggtggg
     3181 accgtatccc aatctgtttg ttcgcccgac ggcgacgccc acacccgagg gctggacacc
     3241 ggagcctacg acgacggcga cacctcgatt cggccggcgc tggcgcaccg aactggtcga
     3301 gctgtacggc tcgcccgagg aaaaccccga cttctggaac tccatatcgt cgaactcata
     3361 cctcagcgat gtctctggcc caatccagct tcatcacggc acgagagatg ccagcgtgcc
     3421 gctggcggcc tcggaaaccc tgtaccggca gatgctggaa gccggtcagc cggtcgagct
     3481 gtatacctac gaaggcgatg accacaatct ctcccagagc tttggattgg cgatgcagcg
     3541 tacaattgag ttcttcaatc gatacctcaa agggccatag ccaggtgata ttcagcagct
     3601 ccccacactg gccgctagcg gtgacgctga ggaggaatcg agttgatgaa agcagggaca
     3661 tttccacatc gctggatcgt cgtcatcgga ttggcgctgc tgatggcggc gtgcgggagc
     3721 aatccccagc cgccgcagac gacgcgcgtg gccgaggtcc cgccgaccgc gtcgcgcacc
     3781 gctgcgcctt ctccaacgga tacacccaca cccactcata cgccgacggc gacgcctacc
     3841 tctacgccga cgccgcatcc gctcacgatt gtttccatgc ggcagcgcga atacccgggc
     3901 agcgacatca ccatcgaaga aacgctgcag ccgggcggta actacagccg ctatattgcg
     3961 tcctacgagt ccgaaggact gacgatctac gcgctgttga ccgtgcccaa cggcgagagg
     4021 ccggcgacgg ggtggccggt catcgtcttc aatcacggct ttatcccgcc cgacgtgtat
     4081 cgcacgaccg agcgctacgt ggcgcacgtg gactggctgg cgcgaagcgg ctacatcgtc
     4141 ctccgcccgg attatcgcgg gcatgacaac tcggagggcg aagcaagagg cgcgtacggc
     4201 tctcccgatt acgtcgtcga cgtgctcaac gccgtcgctg caatgaagcg atacccgaac
     4261 gccgatccga accgcatcgg catgtggggc cattcgatgg gtgggtacat cacactgcgc
     4321 tcgatggtca tcgacggcga cgtaaaagcc ggtgtgatct gggccggcgt ggttggatcc
     4381 tatccggata tgatcaaccg ctggcgccca acgccgactc ccgggccaac cacaaccgcc
     4441 acacccaccg ggcggcggcg ctggcgcacc gagctgatcg ccacgtatgg ctcgccggaa
     4501 gagaaccctg agttctggag ctcgatttcg gcgaactcgt atctcggcga tctgtccggt
     4561 ccgattcaac tgcatcacgg cacggcggat agaagcgtgc cgatcgggtt ctcggagact
     4621 ttgtaccagg agattctggc tgccggcaag ccggccgagc tgtacaccta ccaaggcgcc
     4681 gatcacaaca tcgcaaacag cttcggcctg gccatgcagc ggacgatcga attcttcgat
     4741 aagtatgtca aagggtcgta gcgccttgac cataaggcaa tagccattct cgggtgatta
     4801 cccgcaagtc cgtgcgctcg cagttagact gcgggcgccg ggcgacagtg caactgtcgc
     4861 ctcaacatgc atcaccagac gacgcatact cccgcgataa gcgggagtgt gctatattag
     4921 cgccgtatgg tcgaggcatt cctgcaactg tcgcccgttg cgcaggccat tctggcaacg
     4981 ggcttcacgt ggggtatgac ggcgctcggc gcgggcatgg ttttcctgtc gcacagcgtg
     5041 aaccaaaaga tgctggattg gatgctgggg ttcgccgccg gcgtgatgat cgcagcgagc
     5101 tatttctcgc tgctggcccc ggcgattgcc gcggcggagg gcggcccgct cccggcatgg
     5161 tttccggcgg cgacgggctt cctggccggg gcgatcgtcc tgcggctgat cgacaggatc
     5221 ctgcctcacc tgcatcccgg cttgcgcatc gatcaggcgg aaggcgtcaa gacgacctgg
     5281 cagcgcagcg tgctcctggt gctggctatc acgctgcaca acttcccaga gggcctggcg
     5341 atcggtgtgg cattcggcgc ggcagcggcg ggcatgccgt cggcgacggt cgcgtcggcc
     5401 atcgcgctgg cgatcggcat cggcatccag aactttcccg aaggcacggc ggtggcgatg
     5461 ccgctgcgcc gcgaaggtgt atcgcgctgg aggagttttc tgtacgggca ggcgtcggcg
//
LOCUS       DBNHXB010000268         9114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1410487, whole genome shotgun sequence.
ACCESSION   DBNHXB010000268 DBNHXB010000000
VERSION     DBNHXB010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9114
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1410487"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            584..871
                     /locus_tag="AC162G_10905"
     CDS             584..871
                     /locus_tag="AC162G_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5076371.1"
                     /translation="MVSLQWSPDGRYLLARGGLAGVYLIRPDSENPIAQLDFFGVPEV
                     SQAWAPDGQRFAVLISLGETSRIGIYDVDAAQLSELPVEVQRPFDLAWSTR"
     gene            complement(875..1636)
                     /gene="rlmB"
                     /locus_tag="AC162G_10910"
     CDS             complement(875..1636)
                     /gene="rlmB"
                     /locus_tag="AC162G_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR5076372.1"
                     /translation="MRELIYGRNAVHEVLRAHRREVYKLLVADGAQERGVLAEALALA
                     AQRNIPIQRVPRSRLDHVTDAHQGIAAEAGPYPYVNVEDILDLAAGRNEPPLILVLDV
                     IQNPQNLGTLLRTAEAMGVHGAILPQRRGVEVTLAVVNASSGASEHLRIARVVNLVRS
                     MEELKELGVWIAGLEHKDERAIDFWSADLRGPLALVVGNEAEGMRRLVRQTCDYLLRI
                     PMRGHIESLNASIAGSIVLYEVFRQRRAATQTVAQ"
     gene            complement(1694..3142)
                     /gene="cysS"
                     /locus_tag="AC162G_10915"
     CDS             complement(1694..3142)
                     /gene="cysS"
                     /locus_tag="AC162G_10915"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011356974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR5076373.1"
                     /translation="MPIQIYNVLTRKKEEFVPLNPNLVTMYVCGPTVYDHAHIGHAKT
                     YVSFDVIVRYLRFRGYRVRYVQNITDVGHILDTGEDRILKGARRDRVEPMELVETYTR
                     SYFEDMDALGIVRPDISPRASAHITEQIEMIRKLIEKGHAYEVDGSVYFSVPSFPEYG
                     KLSGRRIEEEEEHARVAVREEKRHPADFALWKKAEPEHILHWPSPWGWGYPGWHIECS
                     AMSTKYLGGPFDIHGGGIDNIFPHNECEIAQWEAAADQPFARYWMLTGSLTLDGVKMS
                     KSLGNVLTIKDALKRYSPQAIRFFVLSSHYSNPIDFSDEAVEAATRGVERLLGPARQV
                     RDRLRTAQDSAGAGEFVRVLDEYRLAFVEAMDEDFNAPAALGVMSKLTTDVNTLLNSG
                     DPIGRATLEAIDKTYRDLGGSVLGVVPEAFKSEANMDVLDGLVRILIDVRAEARKNKD
                     FARADAIRSQLAEAGIALEDGRDGTTWRLTKG"
     gene            complement(3281..4342)
                     /locus_tag="AC162G_10920"
     CDS             complement(3281..4342)
                     /locus_tag="AC162G_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HIR5076374.1"
                     /translation="MTTEFVARIMGMIVFSIVGLQAGVVIVDLASVAPELRVPYVLVF
                     TLVGALAGAILTPWITTRPLNVLRKRIRQVPASQIVAAVIGLIVGLLIAVLLAFPLSF
                     LPSPFREVLPIVVAVGFGWLGMYTMIARQHDIAVLLGGRLSLRESKDASPAHAERHML
                     LDTSVIIDGRIADISQTGFLSDTILVPRFVLNELQHIADSSDSLRRNRGRRGLDILNK
                     LQKDSRAPIRITDMDVEDVREVDDKLILLAKRLKCPILTNDFNLNRVAELQGVRVLNI
                     NELANAVKTVFLPGEGMQVHVIQEGKEIGQGVGYLDDGTMVVVEDGRRHMNATIPVVV
                     TKVLQTAAGRMIFARPDAN"
     gene            4743..5294
                     /locus_tag="AC162G_10925"
     CDS             4743..5294
                     /locus_tag="AC162G_10925"
                     /inference="COORDINATES: protein motif:HMM:NF014704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR5076375.1"
                     /translation="MIVNFILDFLLLLGGVGGLLIGLRRGFVRMLLSTATLLLAMAFA
                     ALMAPPLVGIFVARSGSQAETPIGVVFAALLAGLYALLEVLLRRTFPNTRIAMLGVAD
                     NALGFVFAIPWTLLVLSLIVMLAGFVSYAVYGATGAGFIGDWYSTSNLVAFLQDFFEI
                     PLNLMRFLFPSGLPQPLAFFAAN"
     gene            5463..7862
                     /locus_tag="AC162G_10930"
     CDS             5463..7862
                     /locus_tag="AC162G_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIR5076376.1"
                     /translation="MNAKHWTTLELPKVLARLAKYTSFSAGHERALALAPSPDPDTVA
                     RWQRETSEARRLLTSKADLNLGGVHDVRPLAEGASRNGTLLPQELLDVRSTLIAAREL
                     RRTLTRLDNPFPSLAAVAGRIADLPGLINEIGRCIDAERAEVYDHASPALAAVRRDLR
                     GAFGNLQEKLQRILTNPRNGPYLQEALLTQRGGRYVIPIKAEFKGRIPGIVHDQSASG
                     ATLFIEPLSTVELNNKWRELQLKEEREVQKVLHDLSQIVGAEAAPIARTVEALAEIDV
                     IFARARYADATRSVEPALRAPPVIPPAERGGQRGGAPSGGPVALKQARHPLLDPSTVV
                     PLDIVLDEDTRIVVITGPNTGGKTVALKTVGLIASMAQCGMHIPAAEGSALAIFDQIF
                     ADIGDEQSIEQSLSTFSSHLTNITSFLDEVDDRSLVLLDELGAGTDPAEGSALARAML
                     DHLRARQAVTLVATHYPELKVWAAARHTPGAVNASVEFDAETLRPTYKLTIGLPGRSN
                     AFAIATRLGLPDEIVSRARSMVAEGDLRAEDLLADIHRQRELAESARRAAEAARAEAE
                     RQARELRDRLAKIEDERAAILDRARDDAAQQLAALSEEVDALRKRLSAAQQAELEAVR
                     ARAAELEAKIEPERPADTQHATRNTSHAIRVGDTVWVRPVNATGEVLSIAGREAEIGV
                     GAARMRAKLGDLEWRSSPAPGVGRAGQTTVHLSTRPKLDLDLRGLRADEAIRELEKHV
                     EAAYLAGMPFTRIIHGKGTGSLRKAVREALRDNPVVSAIESGQEGEGGDGVTVVRLVE
                     G"
     gene            7870..9114
                     /locus_tag="AC162G_10935"
     CDS             7870..9114
                     /locus_tag="AC162G_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR5076377.1"
                     /translation="MITDVKPFLQLAVTLPIIILAAKAGGWVSVRLRQPAVLGELLVG
                     LILGPSLIDLLHLPFVFDPDLLEQEIVDLAEIGVVLLMFVAGMEVDLGEMARSGRVSV
                     FSGVLGVIAPLVLGALVTLPFGYQGQEAFYVGVILTATSVSISAQTLLELGVLRTREG
                     IALLGAAVVDDVLVILLLSVFLALIDGSAGAGAIIVVFVRMIVYLVAAGAIGWLALPR
                     LARWVDRQPISEGLAALVAAIALLFAWSAEVIGGLAAITGAFIAGVGLACSPLRGRIE
                     EKMRAVTYGFFVPIFFVSVGLEANVREITGPVVPFLFVLVGVAILSKMLGSGLGARLG
                     GFTNREALRVGVGMVSRGEVGLIVASIGVLQDIIQQELFSVIVILVLLTTLVTPILLR
                     AVFPRTQARRPAPPGRVAVEEN"
BASE COUNT         1517 a         2834 c         3019 g         1744 t
ORIGIN      
        1 cttgttcatt tgagggaacc atgccgccct tttggtacac aaggtgtgaa gctgactcgt
       61 ggattctata gacaaatcct ctggttgatg aattcgtagg caatgtgtat tatttccaaa
      121 cctactagta taacattgac cgcgggtttt gccggtctga tgtggggatt gttgcgcatt
      181 gagttggttc tgctgctgcc ttatttctat ctgttcgtgt tcttatgtga tggtgcggtc
      241 tatatcttgt tgggacacac gattgggcga tgtatggccc tctctcaacc gagaaagcgc
      301 tgggtattat ggctaccttt gctgatcgct tacgtgagcg atcgctttct gtctgctaga
      361 atccttttag gaacatcctt tgccggacgt gttacatcgt tgtgggaact actgatcgag
      421 gtcctgattg ttgggctagc cattttatcc ttgtggagag gctggaggtt aacccaatcc
      481 acaatgtctt cgtagacacc atccgttaga ccggtttcaa acggcgctgt atctggtcga
      541 tctccatact ggtaacaggc gctacctcgg attcgacccc ggcttggtta gcctgcaatg
      601 gtcgcctgac ggtcgatact tgctggcgcg tggcgggctg gccggggtct atctcatacg
      661 acccgactcc gaaaatccta tcgcgcaact cgacttcttc ggcgtgcctg aggtgtcgca
      721 ggcgtgggca cccgatggtc agcgcttcgc cgtgctcatt agcctcggag agacgtctag
      781 aatcggcatc tacgatgtag atgcggcgca attgagtgag ttgcccgtcg aagtgcagcg
      841 ccccttcgac ctggcgtgga gtacccgtta gacttcattg cgcaaccgtc tgggtcgccg
      901 ctcgccgctg ccggaacacc tcgtacaaca caatcgaccc ggcgatcgac gcgttgagcg
      961 attcgatgtg cccgcgcatc gggatgcgca gcagatagtc gcacgtctgg cgcaccaggc
     1021 ggcgcatgcc ttcggcttcg ttgccgacga cgagcgccag tggcccgcgc aagtcggccg
     1081 accagaagtc gattgcccgc tcgtccttgt gctccagccc ggcgatccat actccaagct
     1141 ccttgagttc ctccatcgaa cggaccagat tgaccacgcg cgcaatgcgc aggtgctcgc
     1201 tcgcgccgga cgaggcgttg acgacggcga gcgtcacttc gacgccgcgc cgctgcggca
     1261 ggatcgcgcc gtgaacgccc atcgcttcgg ccgtgcggag gagcgtgcca aggttctgcg
     1321 gattttggat gacgtcgagg acgaggatga gcggtggctc atttcgcccg gcggcgagat
     1381 cgaggatgtc ttcgacgtta acgtagggat acggtccggc ttcggcggcg atgccctggt
     1441 gcgcgtcggt gacgtgatcg agccgcgaac gaggaacgcg ctgtatcggg atgttgcgct
     1501 gcgcggcgag ggcaagcgct tcggccagca cgcccctctc ctgcgcccca tcggcgacga
     1561 ggagcttgta cacctcgcgc cgatgggcgc gcagcacttc gtgcacggcg ttgcggccgt
     1621 agatcaattc acgcataaaa caagggatga gggataaagg atgaaggatg aagttgtcct
     1681 catccttccg cattcatcct ttggtcagcc gccacgtcgt gccgtcgcgc ccgtcttcga
     1741 gcgcgatacc ggcttcggcg agctggctgc ggatcgcatc ggcgcgggcg aagtccttgt
     1801 ttttgcgcgc ttcggcgcgc acgtcgatca gtattcgcac caggccgtcg agcacgtcca
     1861 tgttcgcctc ggacttgaac gcttcgggca cgacgccgag gacactgccg cccagatcgc
     1921 ggtaagtctt gtcgatcgct tcgagcgtcg cgcgaccgat cggatcgccg ctgttgagca
     1981 gcgtattgac gtcggtggtc agcttgctca tcacgccgag cgcggccggc gcgttgaagt
     2041 cctcgtccat cgcttccaca aaggcaaggc gatactcgtc gagcactcgc acgaactcgc
     2101 cggcgccggc ggaatcctgg gccgtgcgca ggcggtcgcg cacctgacgg gcggggccta
     2161 acagtcgctc gacgcctcgc gtggcggctt ccactgcctc gtcgctgaag tcgatcgggt
     2221 tggaatagtg gctgctcagg acgaagaagc ggatcgcctg tgggctgtaa cgcttgaggg
     2281 cgtccttgat cgtcagcacg ttgcccagag acttgctcat cttcacgccg tcgagcgtga
     2341 gtgagccggt cagcatccag tagcgggcga acggctggtc cgccgccgct tcccactgcg
     2401 cgatctcgca ctcgttgtgc gggaaaatgt tgtcgatgcc gccgccgtgg atgtcgaacg
     2461 ggccgccgag gtacttggtc gacatagccg agcattcgat gtgccagccg ggataacccc
     2521 agccccacgg gctgggccag tgcaggatgt gctccggctc ggccttcttc cacagtgcga
     2581 agtcggcggg gtggcgcttt tcctcgcgca cagccacacg cgcatgttcc tcttcttcct
     2641 cgatgcgccg tccggagagc ttgccatact cggggaacga cgggacggag aaatatactg
     2701 agccgtccac ttcgtacgca tggcctttct cgatcaactt ccggatcatc tcgatctgct
     2761 cggtgatatg cgcgctggcg cgcggcgaga tgtcgggccg cacgatgccc agcgcgtcca
     2821 tgtcctcgaa gtacgatcgg gtgtacgtct ccaccagctc catcggctcg acgcgatcgc
     2881 ggcgcgcgcc cttgaggatg cgatcctcgc cggtgtcgag gatgtggccg acgtcggtga
     2941 tgttctgcac gtagcgcacg cgatagcccc ggaagcgcag gtagcgcacg atcacgtcga
     3001 acgagacgta ggtcttggcg tggccgatgt gggcgtgatc gtagaccgtc ggcccgcaca
     3061 cgtacatcgt caccaggtta ggattgagcg ggacgaattc ctctttcttg cgcgtgagga
     3121 cgttgtagat ctggatgggc attggctcgg ttccttggca gtttgaagtt ggaggttggg
     3181 gcttggaagt ttgaagttgg aggttagaag ttggagggcg tcatccaatc tccaatctct
     3241 aacctccaat ctccaatctc taacgtccaa cccccaatct ctagttggca tccggccgtg
     3301 cgaagatcat ccggccggcg gccgtctgca ggactttggt gacgacgacc gggatcgttg
     3361 cattcatatg gcggcgacca tcctcgacga cgaccatcgt gccgtcgtcg agatagccga
     3421 cgccctggcc gatctccttg ccttcctgaa tgacatgcac ctgcatgccc tcgccgggca
     3481 ggaagaccgt cttcactgcg ttagccagct cgttgatgtt cagcacgcgc acgccctgca
     3541 gctcggctac gcggttcagg ttgaagtcgt tggtcaggat gggacacttc aggcgcttgg
     3601 ccaggaggat cagcttatcg tcgacctcgc gcacgtcctc cacgtccata tccgtgatgc
     3661 ggatcggcgc gcgggagtcc ttctgcagct tgttgaggat gtccaatcct cgccggcccc
     3721 gattgcgtct cagcgagtcc gacgagtcgg cgatgtgctg cagctcgttc aggacgaagc
     3781 gcggcacgag gatcgtatcg gacaggaacc ccgtctggct gatgtcggcg atgcggccgt
     3841 cgatgatcac ggacgtgtcg agcagcatgt gccgctcggc gtgagccggg ctggcatctt
     3901 tcgactcgcg cagcgacagg cgaccgccga gcaggacggc gatgtcgtgc tggcgggcga
     3961 tcatcgtgta catgcccagc cagccgaagc cgacggcgac gacgatcggc aatacttcgc
     4021 ggaagggcga gggcagaaac gacaggggaa acgccaggag cacggcgatg agcaggccga
     4081 cgatcagccc gatcacggcg gccacgatct gggaagcagg cacctgccgg atgcgctttc
     4141 gcagcacgtt gagcggccgc gtggtgatcc atggcgtgag gatggcgccg gccagcgcgc
     4201 cgaccagggt aaacaccaac acgtacggca cgcgcagctc cggcgccacg ctggccaggt
     4261 cgacgatgac gacgccggct tgcagcccga cgatgctgaa aacgatcatg cccatgatgc
     4321 gggcgacaaa ttcggttgtc attccggccc cctcgcagcc actgcgcgcc gctaaaacaa
     4381 aaacggcgag cacacctcac ggcgcactca cctgcaatgg ggcagccagc gctcgcacgg
     4441 ggcggtcaag cccgattatg gcgatgtcgt accaccacga agccggctag gcgacggatt
     4501 aggaatgatg gcgcacttga atctgaccgc tcggtgtgag agcaacggac aattgctgga
     4561 tgagtgcaat gcgaataagc gcatacaaag tttatcacgc gcaatgtgaa atgtcaaaca
     4621 ttttcgaacg caagggtcat tttacacgtg tgcgcgaaca cccgtttgcg gacggcgatg
     4681 gatatgctat actcagtctt gcgcaccggg cgcacgcggt ctgaaatcgg cgcggaccgg
     4741 ccatgatcgt caacttcatt ctcgatttcc tgctgttgct gggaggtgta ggtgggctac
     4801 tcatcgggct gcggcgcggc ttcgttcgca tgcttctgag cacggccacc ttgctgctgg
     4861 cgatggcctt tgcggcgctg atggcgccgc ccctggtcgg catcttcgtg gctcgctcgg
     4921 gaagccaggc cgagacgccg atcggcgtcg tgttcgccgc tctcctggcg ggcctttacg
     4981 cgctgctcga agtgctgctg cgccgcacgt ttcccaatac gcgcatcgcc atgctaggcg
     5041 tcgcggacaa cgcgctgggt ttcgtgttcg ccattccctg gacgctgctg gtgttgtcgc
     5101 tcatcgtcat gctggccggc ttcgtcagct acgccgtgta cggggcgacg ggcgccggct
     5161 tcatcggaga ctggtattcg acttcgaatc tcgtcgcatt cttgcaggat ttctttgaaa
     5221 tccctttgaa cctgatgcgg tttctattcc cgtcggggct tccacagccg ctcgccttct
     5281 tcgccgccaa ctgatccgcg ccgtgcaaac aacgcatagg ctcatgcggc gtaactgctc
     5341 acccttgcga aggtttagag tgagtttgcc gtcagaagcg ctggtgcccg tctggcacgc
     5401 acgccggccg gcgcatttga aaccttcgca agggtctgcc tgagtacttg catcgtgaga
     5461 ccgtgaacgc gaaacactgg actacgctcg agctgcccaa ggtgctggca cgcctggcga
     5521 aatacacatc cttctcggca ggccacgaac gggcgctcgc gctggcgccc tcgcccgacc
     5581 cggacaccgt cgcgcgctgg cagcgcgaga cgagcgaggc gcgtcggctc ctgacgagca
     5641 aggccgacct taacctgggc ggcgttcacg atgtgcgccc tctggccgaa ggggcgtccc
     5701 ggaacggcac gctgctcccg caagagctgc tggacgtgcg cagcacactg atcgccgcgc
     5761 gcgagctgcg ccgcacgctg acccgcctgg acaacccatt tcccagcctg gctgcggttg
     5821 ccgggcgcat cgccgatctg cccggcctga tcaacgagat cgggcgctgc atcgacgccg
     5881 agcgcgccga agtgtatgac cacgccagcc cggcgcttgc cgccgtccgg cgcgacttgc
     5941 gtggggcgtt cggtaacctt caggaaaagc tccagcgcat cctgaccaat ccgcgcaacg
     6001 ggccgtactt gcaggaggcg ctcctcaccc agcgcggtgg ccgatacgtc atcccgatca
     6061 aggccgagtt caaggggcgc ataccgggca tcgtgcacga ccagtcggca tcgggcgcga
     6121 cactgtttat cgagccgcta tccaccgttg agctgaacaa caagtggcgc gagcttcagt
     6181 tgaaggaaga gcgcgaggtc caaaaggtgc tgcacgacct gtcgcagatc gtcggggcgg
     6241 aagcggcgcc catcgcgcgc acggtcgagg cgctggccga gatcgacgtg atctttgcgc
     6301 gcgccaggta cgccgacgcg acacgcagcg tggaaccggc gctgcgcgcc ccccctgtca
     6361 tcccccccgc cgagcggggg ggacagaggg ggggcgcgcc aagtggcggc ccggtcgctc
     6421 tgaagcaggc gcggcatccc ctgctcgacc cgtcgaccgt cgttccgctt gatattgtcc
     6481 tcgacgagga cacgcgcatc gtcgtcatca ccggcccgaa caccggcggc aagacggtgg
     6541 cgctgaagac cgtcggcttg atcgcgtcga tggcccagtg tggcatgcac attccggctg
     6601 ccgagggatc ggcgctcgcg atcttcgacc agatcttcgc cgacatcggc gacgagcaat
     6661 cgatcgagca aagcctgtcc acgttctcat cgcacctgac gaacatcacg tctttccttg
     6721 acgaggtcga cgatcgctcg ctggtgctgc tggacgagct gggggcgggc accgatccgg
     6781 ccgaaggctc ggcgctggcc cgcgcgatgc tcgaccacct gcgcgcccgc caggcggtca
     6841 cgctcgtcgc cacgcactat cccgagctga aagtgtgggc cgctgcgcgc cacacacccg
     6901 gcgcggtcaa cgcctcggtc gagttcgacg cggagacgct gcgcccgact tacaagctca
     6961 ccatcggcct gcccggacgc agcaacgcgt tcgcgattgc gacgcggctg ggactgcccg
     7021 acgagatcgt ctcgcgcgcg cggtcgatgg tggccgaggg cgatctcaga gccgaagacc
     7081 tgctggccga catccatcgc cagcgcgaac tggccgagtc ggcgcggcgc gcagccgaag
     7141 cggcgcgcgc agaggccgag aggcaggccc gcgagctgcg cgatcggctc gccaagatcg
     7201 aggacgagcg cgcggcgatc ctcgaccggg cgcgcgacga cgcggcgcag caactggctg
     7261 cgctgtcgga agaggtggat gcgctgcgca agcggctgag cgccgcgcag caggcagagc
     7321 ttgaggccgt gcgtgcgcgc gcggcggagc ttgaagcgaa gatcgagccg gagcgcccgg
     7381 ccgacacgca acacgcaaca cgcaacacat ctcatgccat ccgggtgggc gataccgtgt
     7441 gggtgcgtcc cgtgaatgcg acgggcgagg tgctctccat cgccggtcgg gaagccgaga
     7501 tcggcgtcgg cgcggcgcgc atgcgtgcga agctcggcga tctcgagtgg cgctcctcgc
     7561 cggcgcccgg cgtggggcgc gcgggccaga cgacggtcca tctatcgacc cggccgaagc
     7621 tcgacctcga tctgcgcggg ctgcgcgccg acgaggcgat ccgcgagctg gagaaacacg
     7681 tcgaagcggc gtacctggcc ggcatgccgt ttacgcgcat catccacggc aagggcacgg
     7741 gcagcctgcg taaggcggtg cgcgaggcgc tgcgggacaa cccggtggtc agcgcgattg
     7801 aatcgggcca ggaaggcgag ggcggcgacg gcgtgacggt cgtgcgactc gtggaggggt
     7861 gatggatcga tgattaccga cgtcaagccg tttctccaac tggccgtgac gctcccgatc
     7921 atcatcctgg ccgccaaggc gggcggctgg gtgagcgtcc ggctgcggca gccggcggtg
     7981 ttgggcgagc tgctcgtcgg gcttattctc ggcccatcgt tgatcgacct gctgcacttg
     8041 ccgttcgtct tcgatccgga tctgctcgaa caagagatcg tcgacctggc cgagatcggc
     8101 gtcgtgctcc tgatgttcgt cgccgggatg gaagtggacc tgggtgagat ggcgcgcagc
     8161 gggcgcgtgt cggtgttcag cggcgtgcta ggggtgatcg cgccgctggt gctgggcgcc
     8221 ctggtgacgc tgccgttcgg ctaccagggc caggaggcgt tctacgttgg cgtcatcctc
     8281 acggcgacga gcgtcagcat ttcggcacag acgctgctgg agcttggcgt gctgcgcacg
     8341 cgcgagggca tcgcgctgct cggcgcggcg gtggtggacg acgtgctggt gatcctgctg
     8401 ctgtcggtct tcctggcgct gatagacggc tcggcgggag cgggcgcgat catcgtcgtg
     8461 ttcgtccgga tgatcgtcta cctggtggcg gccggcgcga tcggctggct cgcgctgccg
     8521 cgcctggcgc gctgggtcga tcggcagccg atcagcgaag gattggcggc gctggtggcg
     8581 gccatcgccc tgctgttcgc ctggagcgcg gaggtcatcg gcggcttggc cgcgatcacc
     8641 ggcgctttta tcgcaggcgt cgggctggcg tgcagcccgc tgcgcggtcg gatcgaggag
     8701 aagatgcgtg ccgtgacgta cggattcttc gtgccgatct tcttcgtgag cgtcggcctg
     8761 gaggcgaacg tccgcgagat taccggcccg gtcgtgccct tcctgttcgt gctggtgggc
     8821 gtcgccatcc tgtccaaaat gctgggcagc ggtctgggcg cgcgcctggg cggctttacc
     8881 aatcgcgaag cgctgcgcgt gggggtgggc atggtgtcgc gcggcgaagt aggcctgatc
     8941 gtcgcgtcta tcggcgtgct gcaggacatc attcaacagg agcttttctc ggtcatcgtc
     9001 attctcgtgt tgctcaccac gctggtcacc ccgatcctgc tgcgggcggt ctttccgcga
     9061 acgcaggcca ggcgccctgc gccaccaggc cgggtggccg tggaggaaaa ttga
//
LOCUS       DBNHXB010000269         3983 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_739431, whole genome shotgun sequence.
ACCESSION   DBNHXB010000269 DBNHXB010000000
VERSION     DBNHXB010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_739431"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="AC162G_10940"
     CDS             <1..830
                     /locus_tag="AC162G_10940"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF016119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HIR5076378.1"
                     /translation="FGNEVALGAAIVQAAESLPQPAPDRPPAQAEIVPPPAVLPETPL
                     QEDTQAAQATLALTPTRANPSPTVEPTALPADPSPTVEPTSPPAGPSPTIEPTPTSQS
                     APAPTQTSADFVPDVPTRLTIPAIGLDAPVETVGWSMKVQDGQQVSMWDVPNRFASGW
                     LKTSARAGEKGNTVLDGHHNIAGEVFRDLVQLKAGDTLQLWVGNQSREYIVSFLKILP
                     ERGQPIAVRLENAKWIQPTKDERVTLVTCWPYTNNTHRLIVVALPADAPPPPELGAE"
     gene            831..1325
                     /locus_tag="AC162G_10945"
     CDS             831..1325
                     /locus_tag="AC162G_10945"
                     /inference="COORDINATES: protein motif:HMM:NF039426.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HIR5076379.1"
                     /translation="MNLLELSPVLRTRRNHGLEHATIHVLSGRHPGQAIAGRSTPYGF
                     YIYGNLDTGDVASAAAEALRRLRGGEPHLAVHPGCGTNYVTAGVFAGLGAFVALSIGG
                     RRRWERLPNAIMATTLALILATPLGPMLQARVTTTGDMADLEILSVRKLPGRPMHYVA
                     TRST"
     gene            1424..2422
                     /gene="holA"
                     /locus_tag="AC162G_10950"
     CDS             1424..2422
                     /gene="holA"
                     /locus_tag="AC162G_10950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR5076380.1"
                     /translation="MFYILHGEDEFSRAEQVAAFKEKIGDATVRDFNVTVLDGRKATL
                     AELRLAADAVPFLADKRLVVVEGLLARLGAGKGGKGSSGDELSGAAREFIAQLIDYLP
                     QVPDSTRLVFVEARTLPPSNPILKLAASQPGKTVLAFAIPKNPVPWIEKRALLHGGRI
                     DRKAASKLAQVIGSDLRRLDSEIQKLITYVNAARPIGEDDVARLVSPSIEANIFDLVD
                     ALGRRDGRRAMGELHRLLDLGENSLGLLAMITRQFRLLIQIKELQEKNVPAPEMAKTL
                     GQHPFVIDKIGQQARNFSMEQLERIYVHLLDVDVGIKTGEVGDVLALDLLVAELAG"
     gene            2419..3081
                     /locus_tag="AC162G_10955"
     CDS             2419..3081
                     /locus_tag="AC162G_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR5076381.1"
                     /translation="MKKPFQIDEAVRLIREAIRPWPKAAMFELAEEGFNSPFEQLIAC
                     IISIRTYDEVSLPTARNLFTRARTPADMSQLTIDEIDTLIRECSFHERKAPQIRAIAR
                     RVVDEFGGVLPCDREVLLSFAGVGPKCANLALGVACGEPFISVDVHVHRVTNRWGYVS
                     ASTPERTMTALEAKLPRPYWIEINRLLVPFGKHICTGSLPRCSTCPVLDMCQQVGVKA
                     HR"
     gene            complement(3186..>3983)
                     /locus_tag="AC162G_10960"
     CDS             complement(3186..>3983)
                     /locus_tag="AC162G_10960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5076382.1"
                     /translation="VIERPGANISQGRNAAIEAASGDIIASTDAGVRLDPHWLGVLVA
                     PFGREPQTRVVSGFFAPAPQTAFEVAMGATVLPGRRDIRPETFLPSSRSLAFRKEAWK
                     EAGGYPEWLDYCEDLIFDFALLEKYRPFAFAPEAVVYFRPRGSLRAFVQQYYRYARGD
                     GKANLWIKRHVIRYATYLAAVPGIAALSVVISPWLLLLYLAGAAVYTRTPYRRLRPRL
                     REMTPGDKLKAMALVPVIRVVGDVAKMIGYPVGVTWRIRQRPIRPRR"
BASE COUNT          727 a         1284 c         1261 g          711 t
ORIGIN      
        1 gcttcggcaa cgaggttgcg ctgggcgcgg ccatcgtcca ggcggccgag tcgctgccgc
       61 agcctgcgcc ggaccggcca cctgcgcaag ccgaaattgt gccgcccccg gccgtcctgc
      121 cggaaactcc tctccaggaa gacacacagg ccgcgcaggc gacgctggcg ctgacgccca
      181 ctcgtgccaa cccgtcgccg accgttgaac cgacagcgct tcctgctgat ccgtcgccga
      241 ccgttgaacc gacgtcgcct cctgccggcc cgtcgccgac gattgagccg acgcctacct
      301 cccagagtgc cccagcccca acgcagacga gtgccgactt tgtgccggat gtgccgaccc
      361 gcctcacgat cccggcgatc ggcctggacg cgccggtgga gactgtcggc tggagcatga
      421 aggtacagga cggccagcag gtcagcatgt gggacgtgcc gaatcgcttc gcgtcaggct
      481 ggctgaagac cagcgcgcgc gccggcgaga agggcaacac ggtgctcgac ggccatcaca
      541 acattgccgg cgaggtcttc cgcgatctgg tccaactgaa ggcgggcgac acgcttcagt
      601 tgtgggtagg caaccagtcc cgcgagtaca tcgtgtcgtt cttgaagatt ttgccggaaa
      661 gaggccagcc gatcgcagtg cggctggaga atgccaagtg gatccagccg acgaaagacg
      721 agcgcgtgac gctcgtgacg tgctggccgt atacgaacaa cacgcatcgc ttgatcgtcg
      781 tcgcgcttcc ggccgacgcg ccgccgccgc cagaactagg agcggagtga atgaacctgc
      841 ttgagctgtc gcctgtgctg cgtacgcgcc gcaatcatgg cctggagcac gccacgattc
      901 acgtgctgtc gggacggcat cccggccagg caatagcggg ccgctcgacg ccgtacggtt
      961 tttacattta cggcaacctc gacaccggcg acgtcgcttc ggcggccgca gaagcgctcc
     1021 ggcggctgcg cggtggagag ccgcatctgg ccgtgcaccc cggctgcggc accaattacg
     1081 tcacggcggg cgtgttcgcc gggctgggcg cgttcgtcgc actgagcatc ggcgggcggc
     1141 gccgttggga gcgactgccc aacgccatca tggccaccac gctcgccttg atcctcgcca
     1201 cgccgttggg cccgatgctg caagcgcgcg tcacgacgac gggcgacatg gccgatctgg
     1261 agatcctgtc cgtgcgcaag ctgccgggca ggccgatgca ctacgttgcg acgcgcagca
     1321 cttgatcagt aatcagttat cagttgtcag ttatcagtga acaccaatga gtgagtactg
     1381 gtgactgatc actgatcacg gttcactggt cactgatcat acgatgttct acatcctcca
     1441 cggcgaagac gaattcagcc gcgccgagca ggtcgccgcg ttcaaggaga aaatcggcga
     1501 tgcgacggtg cgcgacttca acgtgaccgt cctcgacggg cgcaaggcca cgctggcgga
     1561 gcttcgcctg gcggcggatg ccgtgccgtt cttggccgat aagcgcctgg tcgtcgtcga
     1621 aggactcctc gcgcggctgg gcgcaggcaa agggggcaaa gggtcgagcg gcgatgagct
     1681 atccggcgcg gccagggagt tcatcgccca gttgatcgac tatctgccgc aggtgcccga
     1741 ttcgacgcgg ctggtgttcg tcgaggcgag gacgcttccg ccgtcgaacc ctatcctgaa
     1801 gctggcggct tcgcagccgg gcaagaccgt cctcgccttc gcgattccga agaatcccgt
     1861 tccgtggatc gagaagcgcg cactgcttca cggcggccgg atcgaccgca aggcggcatc
     1921 gaagctggcg caggtcatcg gcagtgacct gcgccgtctc gactcggaga tccagaagct
     1981 catcacgtac gtcaacgccg cgcgccccat tggcgaagac gacgtggcgc gcctggtgtc
     2041 gccttcgatc gaagcgaata tcttcgacct ggtcgacgcg ctgggacggc gcgatggccg
     2101 gcgcgcgatg ggagagctgc accgcctgct cgacttgggc gagaactcgc tcggcctgct
     2161 ggcgatgatc acacgccagt tccggctgct gattcagatc aaggagctgc aagagaagaa
     2221 cgtgcctgcg ccggagatgg cgaagacgct cggccagcat ccgttcgtga tcgacaagat
     2281 cggccagcag gcgcgcaatt tctcgatgga gcaattggag cgtatttacg tgcacttgct
     2341 cgatgtggac gtaggcatca agaccggcga agtgggcgac gtgctggcgc tggatttgct
     2401 ggttgcggaa ctggcggggt gaagaaaccg tttcagatcg atgaagcagt ccgcctgatc
     2461 cgagaggcga tccggccctg gcccaaggca gcgatgttcg agctggccga ggagggcttc
     2521 aactctccct tcgagcagtt gatcgcctgc atcatttcga tccgaaccta cgacgaggtc
     2581 tcacttccga cggcgcgcaa tctgttcaca cgcgcccgca cgccggctga catgagccaa
     2641 ctaacgatcg atgagatcga tacgctgatc cgagagtgct cgttccacga gcgtaaagcg
     2701 cctcaaatcc gggcgattgc gcggcgcgtg gtcgatgaat tcggtggagt gttaccgtgc
     2761 gatcgcgagg ttctgctgtc gttcgcgggt gtagggccaa aatgtgcgaa cctggcgctg
     2821 ggcgttgcct gcggcgagcc gttcatcagc gtcgacgtgc acgttcaccg cgtgaccaat
     2881 cgctggggtt acgtcagcgc ctccacgccg gagcgcacga tgactgcgct cgaagccaaa
     2941 ctcccacgcc cttactggat cgagattaac cgcctgctcg tgccctttgg caagcacatc
     3001 tgcacgggaa gcctgccgcg ctgctcgacg tgtcccgttc ttgatatgtg ccagcaagtc
     3061 ggtgtcaagg cgcaccggta atccagtcgt aattggtact tggtaattgt ttaccgcgtc
     3121 cctgcctgat tcaccgtttg ccgtcactca tctcgaaacg ccgccgcccg atgttctgaa
     3181 gttagttacc gtcgtggcct gatgggacgc tgccttattc tccacgtcac cccaaccgga
     3241 tacccgatca tctttgccac atctccgacc acgcggatga ccggtaccag cgccatagcc
     3301 ttcaacttgt cacccggtgt catctcgcgc aatcgtggcc gcagccgccg gtacggcgtc
     3361 cgcgtataaa ccgctgcgcc cgccagatac aatagcaata accacggact gatgaccacg
     3421 cttaaagctg caatgcccgg cacggcggcg agatatgtcg cgtaacggat cacgtgccgc
     3481 ttgatccata agttggcttt gccgtcgccg cgcgcgtacc ggtagtattg ctggacgaac
     3541 gcgcgcagcg acccgcgcgg gcgaaaatag acgaccgctt ccggcgcgaa cgcgaacgga
     3601 cggtatttct ccagcagcgc gaagtcgaag atcaggtctt cgcagtagtc gagccactcc
     3661 gggtatccac cggcctcttt ccacgcttct ttcctgaacg ccaggctgcg gctcgacggc
     3721 agaaacgttt ccggccggat gtcgcgccgt ccaggcagca ccgtcgcgcc catcgccact
     3781 tcgaatgccg tctgcggtgc cggcgcgaag aacccggaga cgactcgcgt ctgcggctcg
     3841 cgcccaaacg gcgcgacgag gactccgagc cagtggggat cgagcctcac gccagcgtcg
     3901 gtcgaggcga tgatgtcgcc ggaggcagct tcgatcgccg cattgcgccc ctgcgagatg
     3961 ttcgcgccgg ggcgctcgat gac
//
LOCUS       DBNHXB010000270         4675 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1410939, whole genome shotgun sequence.
ACCESSION   DBNHXB010000270 DBNHXB010000000
VERSION     DBNHXB010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1410939"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="AC162G_10965"
     CDS             complement(<1..525)
                     /locus_tag="AC162G_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076383.1"
                     /translation="MNNSGLERNPVATSPLTQPNTQTLAARLKVSTQWKVAARAIALF
                     VLFAALFAAVQFATPALAGNDGYYHMKMGLLVREQGLKPLFVWLPLTILNREAFYDHH
                     LLYHAYLSLFAFGDSGEALTLGAKIASVIMPALAFVAIWHLLRGQGVCWASLWALGLF
                     AVSEAFLYRMSMPRA"
     gene            complement(690..1019)
                     /locus_tag="AC162G_10970"
     CDS             complement(690..1019)
                     /locus_tag="AC162G_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5076384.1"
                     /translation="MSDATIFTALADPMRRKLLVNLAENSPKTATQLAKEYPRITRQG
                     ILKHLNILEDAGLVAAHRKGREKRYILTPEPLGDLEQWIKEISAKWDERLLRLKTFLE
                     NETAGEK"
     gene            complement(1120..1506)
                     /locus_tag="AC162G_10975"
     CDS             complement(1120..1506)
                     /locus_tag="AC162G_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5076385.1"
                     /translation="MKIQLASVIVDDPIKAFKFYTEVMGFIERVYMPEARLAIVASPE
                     EPGGTGLLLEPNDNPIAKTFQEGVYQQGLPVIVFGVEDIYKEYDRLKKLGVVFRSEPT
                     PQGPFLVADLEDRCGNIVQISQFAPA"
     gene            complement(1562..2011)
                     /locus_tag="AC162G_10980"
     CDS             complement(1562..2011)
                     /locus_tag="AC162G_10980"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5076386.1"
                     /translation="MEKLAVERTVWIAAPRERVWQAITDPEQVEKWFSPGTSWKLSAL
                     EVGGRLYAQGADAELYVHIIEALDPPHRIVLRSAPEPPATPEVTTYTLEEENNGTRLT
                     LTYTGYELMPEELRRKRMDQDGMGFAMMLENLQSHIEGRSLPYPQGF"
     gene            complement(2219..2866)
                     /gene="lexA"
                     /locus_tag="AC162G_10985"
     CDS             complement(2219..2866)
                     /gene="lexA"
                     /locus_tag="AC162G_10985"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR5076387.1"
                     /translation="MMSLSTRQQAMLEFIQRFTRDHRYPPTIREIGQEVGISSTSVVN
                     YNLNILEREGLIERDKEVSRGIKLIGAIAQALDPRSLVSVPLLGRIAAGEPIPVPPSD
                     FATMGDETIELTRGLLPADMTGIYALQVRGTSMVDAMIGDGDIVVMKHQNTARNGELV
                     AVWLRDQGETTLKRFYLENGRVRLQPENPTMKPIYQDPANVEIQGKVVLVVRRPE"
     gene            3129..4301
                     /locus_tag="AC162G_10990"
     CDS             3129..4301
                     /locus_tag="AC162G_10990"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR5076388.1"
                     /translation="MTTAPLTSVRGLEGVVAAETRMSHVDGLAGELIIGGYALEELAG
                     RATFEEACFVLWRLALENKASLPNRAQMDALRQEMAALRALPPLVHSTIKAAKDAPPM
                     DALRMSVAALSLDDPNAKDESLAANLRRAKLLTARVPAIIAAHDRARRGLDSIPPRAD
                     LPRAANFLYMLDGKDPDMHAARALDTYWTTVIDHGMNASTFTARVIASTQSDMVSAVT
                     GAIGALKGPLHGGAPGPVLDMLVEIGSVDRAEEWVRGELAQGRRIMGFGHRVYRVRDP
                     RADVLSRAVREMNAAGGGDKALFEMATQVEQIILRLLEEHRPGRNLQTNVEFYTALVL
                     QSIGLRPDQFSAMFAAGRVGGWTAHVLEQLANNRLIRPSSEYVGPRGLKYVPIEQR"
     gene            4399..>4675
                     /locus_tag="AC162G_10995"
     CDS             4399..>4675
                     /locus_tag="AC162G_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR5076389.1"
                     /translation="MNQGIRDFVHCQTIAVVGASRTGKKFGNMAYRDLKKRGYDVLAV
                     HPTAQTLEGDTCYASLRDLPRTPDGVIVSLPPNKVTPVLRDAADLGIR"
BASE COUNT          903 a         1436 c         1403 g          933 t
ORIGIN      
        1 cgccctcggc atgctcatgc ggtacaggaa cgcttcggac acggcgaata atccgagcgc
       61 ccagagagat gcccagcaca ccccctggcc gcgcaggagg tgccagatcg cgacgaaggc
      121 cagggccggc atgatcacgc tggcaatctt cgcgccgagc gtcaatgcct cgcccgagtc
      181 gccgaacgca aaaagcgaca ggtaggcgtg atacaggaga tggtgatcgt aaaacgcctc
      241 gcgattgagg atagtcagag gcagccacac gaacagcggc ttaagcccct gctcgcgcac
      301 gagcagcccc atcttcatgt ggtagtagcc gtcgtttccg gccagcgcgg gcgtggcaaa
      361 ctggacggct gcaaagagcg cggcgaagag cacgaacagg gcaatcgcac gagcggcgac
      421 tttccactgc gtggacacct tgaggcgtgc ggccagtgtt tgtgtgttcg gttgagtgag
      481 cggggaggtt gccaccgggt ttcgttccag gccagaatta ttcaccacgc tacctatagt
      541 cagtcgcgga aagcagcgaa aagtaacgcc ccgttcaaca aaaagcccca gcgcgtcggc
      601 tgggccgggg tatgtcccat ttccgacgct cttggccgct ggattgtcat ccagtgggag
      661 cagcaaattg cattctcaaa atcaggcctt cacttctcgc cagccgtctc attttcgagg
      721 aatgtcttga gccgcagcag gcgctcatcc catttggcgc tgatctcttt gatccattgc
      781 tcaagatcgc cgaggggttc cggcgtcagg atatagcgct tttcgcgccc ctttcgatgc
      841 gcagcgacca agcccgcatc ttcgagaata ttcagatgct tgagtatccc ctgtcgtgtg
      901 atgcggggat attcctttgc aagctgggtc gccgtcttag ggctgttttc cgccaggttc
      961 accagcaatt tccggcgcat cggatcagcc agcgcggtga agatggtcgc gtcactcaca
     1021 acaggctgct cccaccctga ttggtgtaca ggcccccggc gataggtgtc aacgcacatt
     1081 tgacccccta tcgccgggca gcctcactgg cagcggtgac tacgccggag caaactggga
     1141 aatctgtaca atgttgccac atctatcctc aaggtcagcg acgaggaacg gcccctgtgg
     1201 tgttggctcc gaacgaaaca ccacccccag ctttttcagt ctgtcgtact ctttgtaaat
     1261 atcctcaacc ccaaatacga tgacgggtaa cccctgctga tagacaccct cctggaatgt
     1321 cttggcgatg gggttgtcgt ttggctcaag cagcagcccg gtaccccctg gctcttcggg
     1381 tgatgcaaca attgccagtc gcgcttcagg catatacacc ctttcgatga agcccataac
     1441 ctccgtgtaa aacttgaagg ccttgatggg gtcgtctaca atgacactgg cgagttgtat
     1501 cttcatgata gcgtctcctt aggtttgagt gaagttgacc acggatgcct cacatgtgcg
     1561 cttagaagcc ctgtggatag ggtaggctcc tgccctcaat atgggattgc aagttctcca
     1621 gcatcatagc gaagcccatg ccgtcctggt ccatgcgctt ccggcgaagt tcttccggca
     1681 tcagctcata gcctgtgtag gtcagcgtca atcgtgtgcc attgttttcc tcctccaagg
     1741 tgtaggtcgt gacctccggt gtggctggcg gctccggcgc cgaacgcagg acgatgcgat
     1801 ggggcggatc gagggcctcg ataatgtgta cgtagagttc tgcgtccgca ccctgagcgt
     1861 atagcctccc accgacttcc agcgcggaga gcttccagga tgtccccggc gaaaaccact
     1921 tttcgacctg ctctggatcg gtaatagcct gccaaacccg ctcgcgcggc gcggcgatcc
     1981 aaacggtgcg ttcgacggct aacttttcca tgtcatctgt ctccaatgtg caacctttta
     2041 gttgcacata ttgtaacaga aaaatgttct aagtcaatcc cccactttcg gtaggatttt
     2101 cactgaggca acgcgccccc tgttcataga gggcggtact gaaagactag ctgtcgcggt
     2161 gatgctcaaa acaaaagccc cagctagacg gctggggcct gtatgtgggt tggacacatt
     2221 attcgggtcg gcgcacgacc agcacgacct tgccctgtat ctcgacgttc gccggatcct
     2281 ggtagatcgg cttcatcgtc ggattctccg gctggagccg gacgcgcccg ttttcgaggt
     2341 agaagcgctt gagcgtcgtt tctccctgat cgcgcagcca cacggcgacc agctcgccgt
     2401 tgcgcgccgt gttctggtgc ttcatcacga cgatgtcgcc gtcgccgatc atcgcatcga
     2461 ccatcgacgt gccgcgcacc tggagcgcgt agatgccggt catatcggca ggcagcagcc
     2521 cgcgcgtaag ctcgatcgtc tcgtcgccca tcgtcgcgaa gtcggacggg ggcacaggga
     2581 tcggctcgcc ggcggcgatg cggccgagca ggggcacgct caccaggctg cgcggatcga
     2641 gcgcctgcgc tatcgcgccg atcagcttga tcccgcgaga gacttccttg tcgcgctcga
     2701 tcagcccttc gcgctccaga atgttcagat tgtagttgac gaccgaggtc gacgagatgc
     2761 cgacctcttg accgatctcc cgaatcgtcg gaggatagcg gtgatcgcgt gtgaagcgct
     2821 ggatgaattc gagcatcgct tgctgacggg tagataggct catcattcac ctccgcagga
     2881 aatcccaagt atcccaagtg atttcccaaa ccgtgaatgc attataaggg aacattagtt
     2941 ctatgtcaag acccaaacga aacatatggt cgacctgctg ctcaacccca ggcggctgat
     3001 cgccacactg cggcgatagt gtgtgacgac attcgacaat tcgatccgcg tttggtatac
     3061 ttcatcttgt acccgatcat ttccgactcg atacccgttt cgcccgcttc attttcgcag
     3121 gagacgagat gaccacagca cctctaacct cagtgcgggg ccttgaaggc gtggtcgccg
     3181 ccgaaacgcg catgagccac gtcgacggcc tggccggcga gctgatcatc ggcggctacg
     3241 cgctggagga gctggccggt cgcgccacgt tcgaagaagc ctgtttcgtg ctgtggcgcc
     3301 tggcgctcga aaacaaggcc agcctgccca accgtgctca aatggacgcc ctgcggcagg
     3361 agatggccgc gctgcgggct ttgcctccac tcgtgcactc gaccatcaag gccgccaaag
     3421 acgcgccgcc gatggacgct ctgcgcatgt ccgtggcggc gctttcgctg gacgatccga
     3481 atgcgaaaga cgagtcgctg gcggccaatc tgcggcgcgc caagctgctg acggctcgcg
     3541 tgccggccat tatcgccgcg cacgaccgcg cccggcgtgg actcgattcg atccctccgc
     3601 gcgccgacct tccccgggcg gcgaacttcc tgtacatgct ggacggcaaa gaccccgaca
     3661 tgcacgcggc ccgcgcgctc gatacctact ggacgacggt gatcgatcac ggaatgaacg
     3721 ccagcacgtt caccgcgcgc gtcatcgcct cgacgcagtc cgacatggtc tcagccgtca
     3781 ccggcgcgat cggcgcgctc aaggggccac tgcacggcgg cgcgcccggc ccggtgctgg
     3841 atatgctggt cgagatcgga tcggtagacc gggccgagga gtgggtgcgc ggcgagctag
     3901 cccaaggccg gcgcatcatg ggctttgggc atcgcgtcta ccgggtgcgc gatccgcgcg
     3961 ccgacgtgct gagccgcgcc gtccgtgaga tgaacgcggc cggcggcggc gacaaggcgt
     4021 tgttcgaaat ggcgacgcag gtcgagcaga tcatcttgcg tcttctcgaa gagcacaggc
     4081 ccgggcgcaa cctacagacc aacgtcgagt tctacacggc gctggtgctg caaagcatcg
     4141 gtttgcgccc cgaccagttc tcggcgatgt tcgccgcggg acgcgtcggc ggctggacgg
     4201 cgcatgtttt ggagcagttg gcgaacaacc gcctgatccg gccatcgtcg gaatacgtag
     4261 gccctcgcgg gttgaagtac gtgccgatcg agcagcggtg atggcccgcg cggcatctgg
     4321 tgaggtatgg cactaccggg gagttgcctt ccgtagtacc ctgggaggga cgcctgggac
     4381 tcgcatcgga ggtgccgaat gaatcaagga atacgggatt tcgttcactg ccagaccatt
     4441 gccgtagtcg gcgcgtcgcg cacgggcaag aagttcggca acatggccta ccgcgacttg
     4501 aagaaacgcg gttacgacgt gcttgccgtg catcccacgg ctcaaacact cgaaggtgac
     4561 acgtgctacg cttcgctgcg cgatctgccg cgcacgccgg acggggtgat cgtcagcctg
     4621 cccccgaaca aggtgacgcc ggtgctgcgc gacgccgccg atttgggcat tcgca
//
LOCUS       DBNHXB010000271         3474 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4431186, whole genome shotgun sequence.
ACCESSION   DBNHXB010000271 DBNHXB010000000
VERSION     DBNHXB010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4431186"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..659)
                     /locus_tag="AC162G_11000"
     CDS             complement(210..659)
                     /locus_tag="AC162G_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HIR5076390.1"
                     /translation="MSSGPNDLALALALRLRDIARMLDRLDRLESGSGEWLLSQGESA
                     LAEAAREMTLRTFRMAADPVNFAVLAFLSTRTAAPIAELEKASGLGRLALSERVNDLV
                     QVGLIERNIDTDHVQGTAACAALVRLIDGIGQATAKKLSEALKRVTR"
     gene            complement(725..2854)
                     /locus_tag="AC162G_11005"
     CDS             complement(725..2854)
                     /locus_tag="AC162G_11005"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR5076391.1"
                     /translation="MLTTPEIIAFSLLMIVSTTLTIYGFLGIYSVIRRGRQASRLKNL
                     FPRFVVTLIQVGLQQTIFRSRPVLSVFHAFIFFGFSFYFLVNVNDLLEGFVVGYSTAE
                     VSTGLIGVFNIIADVLSVLTLAGMVAFLVRRFVAKDKRLNFNPNVLLHPGVAAGGVRR
                     DSAIVGAFILLHVGSRFLGQALRLAEMGVTNPMQPFASLVSQAFAGLPPDTINLLIHI
                     TWWVAIGLVVVFLPYFVISKHIHLMIAPLNLALAKQTARGHLDRVFPGSEPPPGFAPG
                     AQMLADLPWPRLLDAYACIMCNRCQDVCPAHNSMRPLSPAALEINKRYWINSNFSAFA
                     DGAPSPPLTEFAISPEAVWSCTTCFACVRICPVGNEPMSDIIDIRRRLVNDGAELDGG
                     IQSTLEKIGKTGNSFGQSARNRAKWAQTGLDFKIKDVRKEPAEYLWFVGDTASFDARV
                     QQITRTVARVLHHAGVDFGILYDAEKNSGNDVRRVGEEGLFEQLAEQNVAAMSKCTFE
                     RVITTDPHTLNALKNEYPEYGGKWDTIHYTRLLARLIDEGQIRLARKLDYRVTFHDPC
                     YLGRYNQGFNPPRALIRATGCELVEMPRNKENSYCCGAGGGQIWMGATPPGERPAENR
                     IREALSVLDGRSGDGAKPERPLLFVVACPKDVVMYTDAVKTTGNEGKIEVRDVIQLVA
                     EAMGEQIQTPKSHVQTPKTESQTIVPA"
     gene            complement(2861..>3474)
                     /locus_tag="AC162G_11010"
     CDS             complement(2861..>3474)
                     /locus_tag="AC162G_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR5076392.1"
                     /translation="AEDGTLVATSQIYGGKIQAEAVPNGEACIVSCLAGAFHAEAGRV
                     GATLAVAPPVEQIASPVPIDDLKVKFVRLIEPEGGDVDITQQAMLVSIGRGIGGQENI
                     ELAQELADALGCAVSASRPITDAGWLPKTRQVGKSGLMVKPKVYLAFGISGAPEHLEG
                     MRGAELIIAVNTDPKAPIFDVAHYGATCDILDLMPLLSEKIKG"
BASE COUNT          655 a         1072 c         1090 g          657 t
ORIGIN      
        1 agcccgcaca ccgggcagcc gacgatgtac ctcatcttcc ccgtcgcgtc gtcgtgctcc
       61 gtcagcacct ggtcgagatg cgcctcgccc agatggcgat gcagcttcat acgtgtatct
      121 tcaaaggtgc agtgtgggca tttcatacgg gtaattcgta atgggtaatt caacttaggt
      181 tgcgacccgg ctgccttacg aattaccaat taccgggtaa cccgtttcag cgcctctgac
      241 agtttcttcg ccgtcgcctg gccgatgccg tcgatcaggc ggacgagcgc cgcgcaagcg
      301 gcggtgccct gcacgtgatc ggtgtcgatg tttctctcga tcagcccaac ttgcacgagg
      361 tcgttgacgc gctcggagag cgcgagacgc ccaagtccgg acgctttttc gagttcggca
      421 atcggcgcgg cggtgcgcgt cgagagaaac gcgaggacgg cgaagttcac cggatcagcg
      481 gccattcgaa atgtgcgcag cgtcatctct cgcgccgctt ccgccagcgc gctctcccct
      541 tgtgagagca gccactcgcc cgagcctgat tccaggcgat cgagccggtc gagcatgcgc
      601 gcgatatcgc gcagccgaag ggcaagggca agagcaaggt cgttgggtcc agaggacatc
      661 gggaaaatcc aaacgccaaa ggttcaaact tcaaagttca aactccaaac tccaaaagca
      721 catttcaggc cgggacgatc gtttgagatt cggtttttgg agtttgaaca tgggattttg
      781 gagtttgaat ttgttctccc atcgcttcgg ccaccagttg aatcacgtcg cgcacttcga
      841 tctttccctc attgcccgtc gtcttcaccg cgtcggtgta catgacgacg tctttcgggc
      901 aggcgacgac gaacagcagc ggcctttccg gcttcgcccc gtcgcctgag cgcccgtcga
      961 gtacggagag cgcctcgcgg atgcggttct cggctgggcg ctcgccaggc ggggtcgcgc
     1021 ccatccagat ctgcccgccg cccgcgccac agcagtacga gttttccttg ttgcgcggca
     1081 tttcgaccag ctcgcagccc gtcgctcgaa tcagcgcccg cggcgggttg aagccctggt
     1141 tgtagcgacc gagataacac gggtcgtgga acgtgacgcg gtagtcgagc ttccgggcca
     1201 gccggatctg gccctcgtcg atcaaccgcg cgagcagacg cgtgtaatga atcgtgtccc
     1261 acttgccgcc gtattccgga tattcgttct tcagcgcgtt gagcgtgtgc ggatccgtgg
     1321 tgatgacgcg ctcgaacgtg catttgctca tcgccgcgac gttctgctcg gcaagctgct
     1381 cgaacaaccc ctcctcgccg acgcgccgca cgtcgttgcc ggaattcttc tcggcgtcgt
     1441 acaggatgcc gaagtccaca cccgcgtggt ggaggacgcg tgcgacggtg cgcgtgatct
     1501 gctgcacccg cgcgtcgaac gaagcggtgt ccccgacgaa ccacaggtat tcagccggtt
     1561 ctttgcgaac gtccttgatc ttgaaatcga ggccggtctg cgcccacttg gctcgattgc
     1621 gcgccgactg gccgaatgag ttgcccgttt tgccgatctt ctccagcgtc gattggatgc
     1681 cgccgtccag ctccgcccca tcgttcacca ggcggcggcg gatgtcgatg atgtcggaca
     1741 ttggctcgtt gccgaccggg cagatgcgca cgcaggcgaa gcaggtcgtg cacgaccaca
     1801 ccgcttcggg actgatggcg aactcggtga gcggcggcga cggcgcgccg tcggcgaacg
     1861 cactgaagtt cgaattgatc cagtagcgct tgttgatttc cagcgcggcc gggctgagcg
     1921 ggcgcatcga attgtgcgcc gggcagacgt cctggcagcg attgcacatg atgcaggcgt
     1981 aggcgtccag caggcgcggc caggggagat cggccagcat ctgcgcaccc ggcgcaaaac
     2041 cgggcggcgg ctccgatccg gggaagactc gatcgagatg gccgcgtgca gtttgttttg
     2101 cgagcgccag gttgagcggc gcgatcatca aatggatgtg cttgctgatg acgaagtacg
     2161 gcaggaacac gacgaccagc ccgatcgcca cccaccaggt gatgtgaatg agcaggttga
     2221 tcgtgtccgg cggaaggccg gcgaaagcct gcgacacgag tgaggcaaac ggctgcatcg
     2281 ggttggtcac gcccatctcg gccagccgca gcgcctgacc caggaagcgc gacccgacgt
     2341 gcagcaggat gaacgcgccg acgatcgccg aatctcgtcg cacgccgccg gcggcgacac
     2401 cggggtgcag cagaacgttc gggttgaagt tgagccgctt gtctttcgct acaaagcggc
     2461 gcaccaggaa ggcaaccatg cccgcgagtg tgagcacact gagcacatct gcgatgatgt
     2521 tgaacacgcc gatcaggccg gtcgaaacct cggccgtcga atagccgacg acgaaacctt
     2581 ccagcaagtc gttgacgttg acgaggaagt agaagctgaa tccgaagaag atgaatgcgt
     2641 gaaaaacgct gagaacgggg cgcgaacgga agatcgtctg ctgcaggccg acttgaatca
     2701 gcgtgacgac gaagcgcgga aacagattct tgaggcgcga agcctgacgg ccgcgccgga
     2761 tgaccgaata gatgccgagg aagccgtaga ttgtgagtgt cgtgctgacg atcatcagca
     2821 agctgaacgc gatgatctca ggcgtagtca gcatttggat ttatcctttg atcttctcac
     2881 tcagcagcgg catcaaatcc aatatgtcgc acgtcgcgcc gtagtgggct acgtcgaaga
     2941 ttggcgcttt gggatcggtg ttgacggcga tgatcagctc cgcgccgcgc atgccttcga
     3001 ggtgctcggg cgcgccggag atgccgaatg ccaggtacac cttgggcttg accatcaggc
     3061 cgctcttgcc tacctgccgc gtcttaggca gccagcctgc gtcggtgatg gggcgcgagg
     3121 ccgacacggc gcagcccagc gcgtcggcca gctcctgcgc cagttcaatg ttctcctgcc
     3181 cgccgatgcc ccgcccgatc gacaccagca tcgcctgctg ggtgatgtcc acgtcgccac
     3241 cttcaggctc gatcagcctg acgaacttca ccttcaagtc gtcgatcgga accggcgacg
     3301 cgatttgctc gaccggggga gcgaccgcaa gggtcgcccc tacgcggccg gcttcggcgt
     3361 ggaacgcgcc cgccagacac gagacgatgc acgcttcgcc attcggcaca gcctcggcct
     3421 gaatcttgcc accgtaaatt tgcgaagttg caacgagcgt cccgtcctcg gcgg
//
LOCUS       DBNHXB010000272         8360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2083496, whole genome shotgun sequence.
ACCESSION   DBNHXB010000272 DBNHXB010000000
VERSION     DBNHXB010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8360
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2083496"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1713)
                     /locus_tag="AC162G_11015"
     CDS             complement(49..1713)
                     /locus_tag="AC162G_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5076393.1"
                     /translation="MAKTVSKKAVKKAKTRAVKSVKKAARRVAPGGDGAFKLTYATMF
                     DPPEEMHIRFEAALARVRGELGEDHGMLINGQDRFVEAKFDDRSPINTEWLLGTFQKG
                     SAQDARDALAAARAAWSSWAGMKWKDRVRLLRKAAALIEKRVYEIGAVMAIEVGKNRM
                     EALGDVQETADLISYYCDQMEANAGYIKPMGVDPLKGYKSRNLSVLKPYGVWVVISPF
                     NFPFALAGGPSGAALVAGNTVVFKPASDTPYTGRLLAEVFRDAGLPDGVFNYVTGSGT
                     TVGETLITSPEVDGITFTGSYDVGMHILRTFAGGKWPRPCIAEMGGKNPAIVSRNADL
                     DRAAIGVMRSAFGLQGQKCSACSRIFVEKPVVDQFKQKLLDLTHRISIGDPTRRENWL
                     GPVVNANSYRQYSEFGEELSHSGTILTGGKHLVEGDLGKGYFCAPTIAEAPKDHRLWQ
                     YEMFLPIAMIAEVDSLEEAMRRANGVEYGLTAGFYGSKKELAWFFDNIQAGVTYANRP
                     QGATTGAWPGFQPFGGWKGSGSTGKAGGSLYYVPQYMHEQSQTVIE"
     gene            complement(1788..2108)
                     /locus_tag="AC162G_11020"
     CDS             complement(1788..2108)
                     /locus_tag="AC162G_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076394.1"
                     /translation="MAKGESIERGNTIRFAWSAASGPVRATYRIDVLRYEPGGDRYVC
                     RLVELVSVDRMQPDEAVSDDTLRSLTGKCVRVPREALNGSILPLKMTTLTGDLARSYF
                     FDEA"
     gene            complement(2112..3482)
                     /locus_tag="AC162G_11025"
     CDS             complement(2112..3482)
                     /locus_tag="AC162G_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5076395.1"
                     /translation="MSRPTSSPYPGDADRTVDERAVVMHTWTVQADWGAPTLVGGEGA
                     LFWDDQDNVYIDMSSMAECSNLGHQHPRVVAAIRRQAGELCFATSAWGAKPRAELAAR
                     LIELSGYDGGRVFFTLAGADANENAVKLARWATNKPVGKIITRYRSYHGATLGAMEFS
                     GDARGWVYAPGTPNVVHAVPPYCYRCPLGLAYDKCGIRCADHVADLIEWEGPKTVSAV
                     LMEPHAGTNGVVAPPEYWPRLREICSRYNILLIADEVMSGFGRVGHWFAWQEYGEEGR
                     PDLMTLAKGLTGAHVPLGAVVLSRKVAEQFESQMLYSGMTYSGHPLACAAGLAALDAY
                     RDEDLIARSRDLGAHMHSVLQVLEESHPSIGDIRGQGLFAVIELVRNRQTRGPLSPWP
                     QTAPSLRRLVDEGRKNGVSFAVRGNLIILAPPLNIEQRHLIRALEVLDKLLPMCDAEV
                     ARTAGE"
     gene            complement(3535..4287)
                     /locus_tag="AC162G_11030"
     CDS             complement(3535..4287)
                     /locus_tag="AC162G_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HIR5076396.1"
                     /translation="MADPVSDDGQPISLGAALNLLAHGEIEVQGLMPWSSNYTFLTLV
                     VHGELQVPAVYKPCKGERPLWDFPAGTLAYREVAAHLVSEALGWMLVPPTVLRDGPHG
                     PGMVQFYIHADPDEHYFTFGKQFPEQFKKIVLFDALVNNADRKAGHCLRDRDDHIWAI
                     DHGICFHEEPKLRTVIWEFGGQKIPADLLRDLRAFRRRLDSRSDPVTKSLGDLLAREE
                     VEALRRRADQLIAQARFPSPSHYERHVPWPPV"
     gene            complement(4303..4866)
                     /locus_tag="AC162G_11035"
     CDS             complement(4303..4866)
                     /locus_tag="AC162G_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HIR5076397.1"
                     /translation="MSQRVYELNPVKAITAGAVGEPGHRVFYIQARALDEIVTLIAEK
                     EQVVMLARGVYQFLKEIDEKFPAELEGDSVGPLDLELRGPFEPNFRVATLGLGYDAQN
                     DLVVLVAEELVPEDTESEPGAAAEPRAASVARFWATRSQMRALADHALEIAARGRPQC
                     PLCGRPEDPDGHFCPRRNGHSRTLVTP"
     gene            complement(4877..5509)
                     /locus_tag="AC162G_11040"
     CDS             complement(4877..5509)
                     /locus_tag="AC162G_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HIR5076398.1"
                     /translation="MTQVFLIRHGHNDWADKKLAGWTPGVHLNDAGRRQAEALVERLA
                     PLELEAIYSSPLERTLETAVPLARARGLKVIRMRDLGEVRYGDWQGKSLGQLKKRKEW
                     AIVQMAPSVMRFPNGESLREMQSRAVAGIERIVAAHPKGNVAAFSHGDVIKAIVAHFV
                     GMPLDSFQRLIIAPASVTVLGMGRFGARLLRLNDTGPFAPPSQNGRARQR"
     gene            complement(5602..6672)
                     /locus_tag="AC162G_11045"
     CDS             complement(5602..6672)
                     /locus_tag="AC162G_11045"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR5076399.1"
                     /translation="MQIGIIGLPRCGKTTLFHALAGGEKAVATFSAGPLKIQTAVASV
                     PDARVDILSAMFKPRKPVYAKVMYADVGGLDKGVGKTGLPGPFVNQIGQMDAFVHVLR
                     AFDDPSVPHSEGSVDPLRDRAILDTEFLLNDLGIVERRWERVEQSIKKGAPDREQAAK
                     EKALLARLKETLEAETPLRAVALSAEEEQRLRGFGLLSLKPMLLVFNIGEGGRAPDFE
                     ADSAKTAVVALNARLEHEIIELSANDAALFMQEYGISELSRTKVIRLSYDLLGVQSFF
                     TIGEDEVRAWTVRKNATALEAAATVHTDLAQGFIRAEVVSYHDLVELGSMNEAKASGR
                     LRLEGKEHVVEDGDIVHIRHSN"
     gene            7039..7512
                     /locus_tag="AC162G_11050"
     CDS             7039..7512
                     /locus_tag="AC162G_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076400.1"
                     /translation="MTPSPPAVGKPARITITATNAGTANADNVVITDPVPDNMALAGV
                     TASQGTVVVSRGIVTVYVGTLAPGQTVTVVSDVIITRAFANDTPYTNCTGLTFRDGTA
                     RLACFPFGPAFGPVSVASPPRFLPDAGAPGGWLPLSLILIGSAGLLVGLRLRLRR"
     gene            7516..8331
                     /locus_tag="AC162G_11055"
     CDS             7516..8331
                     /locus_tag="AC162G_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5076401.1"
                     /translation="MPDRPTLLIVEDDDGLRVLLQTALGRDDYDVTAAGSGEEALRKM
                     SILAFDVVITDIRLPGIDGVQLVRDIRRRWPDIVVIVLTGYPALESAVACLRAGVHDY
                     LYKPCPIGEIRRSLREGLAKRHGFARRLELLQGLEQQLTQGLRVLRGEMPATSSMLDA
                     MGDGATTVGRYVRTGSLIIDRERHEAILGETQLDLTPTEFEILGYLAERAPAVVTPVD
                     LARRAMGYDADEQEARELVKWHIHHLRQKIEADATRPILLKNVRGVGYKLDVA"
BASE COUNT         1443 a         2719 c         2623 g         1575 t
ORIGIN      
        1 ctgaaccttt ggttcaggag agggggaggg gatggggagg gggtgaggtt attcgatcac
       61 tgtctgagat tgttcgtgca tgtactgcgg cacgtagtac agtgagccac ccgccttgcc
      121 ggtgctgccg gaacccttcc agccgccaaa cggctggaaa cccggccacg cgccggtcgt
      181 cgcgccctgc ggacgattcg cgtacgtcac gccggcctgt atgttatcga agaaccaggc
      241 aagttccttc ttgcttccgt agaatccagc cgtcagaccg tactcgacgc cgttggcgcg
      301 gcgcatcgcc tcttcaagcg agtcgacttc cgcgatcatc gcgatcggca ggaacatttc
      361 atactgccac aggcgatgat ccttcggcgc ttcggcgatc gtcggcgcgc agaagtaacc
      421 tttgcccaga tcgccctcga cgaggtgctt gccgccggtg agaatcgtcc cgctgtgcga
      481 cagctcttcg ccgaactcgg aatactggcg atacgagttg gcattgacaa cggggccgag
      541 ccagttctcg cgccgggtcg gatcgccgat gctgattctg tgcgtcaaat cgaggagctt
      601 ctgtttgaac tggtcgacca ccggcttctc cacgaagatg cgcgagcagg ccgagcactt
      661 ctgcccttgc aggccgaacg ccgagcgcat gacgccgatc gccgcgcgat ccagatcggc
      721 gttgcggctg acgatggccg ggttcttgcc gcccatctcg gcgatgcacg ggcgcggcca
      781 cttgccgccc gcgaacgtgc ggaggatgtg catgcctacg tcgtacgagc cggtgaaagt
      841 aatgccgtcg acttcagggc tggtgatcaa cgtttcgccg acggtcgtgc cactgccggt
      901 gacgtaattg aacacgccgt cgggtaagcc ggcatcccgg aacacctcgg ccaacaggcg
      961 gcccgtgtac ggtgtatccg acgccggctt gaagaccacc gtgttccctg cgacgagcgc
     1021 cgcgccgctc ggcccgccgg ccaacgcgaa cggaaagttg aacggcgaga tcacgaccca
     1081 cacgccgtag ggtttgagca cgctcaggtt gcggctcttg tagcccttga gcggatccac
     1141 acccatcggc ttgatgtagc cagcgttggc ctccatctga tcgcaatagt acgaaatcag
     1201 gtcggcggtc tcctgaacgt cgccgagcgc ctccatgcga ttcttgccga cctcgatcgc
     1261 catgactgcg ccgatttcat acacgcgctt ctcgatgagc gcagccgcct tgcgtaacag
     1321 cctgacccga tccttccact tcattcccgc ccacgaggac cacgcggcgc gcgccgccgc
     1381 gagcgcatcc cgggcgtctt gcgcgctgcc cttctgaaac gtgccgagca gccattccgt
     1441 gttgatcggg ctgcgatcgt cgaacttggc ctcgacaaag cgatcctgcc cattgatcaa
     1501 catgccgtga tcttcgccca gctcacccct caccctggcc agcgccgcct cgaaccgaat
     1561 gtgcatttcc tccggcggat cgaacatcgt cgcgtaggtc agcttgaacg cgccgtcgcc
     1621 tcctggcgcg acgcgcctcg ccgctttctt gacgctcttc accgctctgg tcttggcctt
     1681 cttcactgct ttcttgctca ctgtcttcgc catggataga cctcctcatt ctgaaagcat
     1741 tcagacgcaa cgctggcgaa ggcgcgcatg ccttcgcgca tcgtcgttca cgcttcgtcg
     1801 aaaaaatacg agcgggcgag atcgccggta agtgtagtca tcttcagcgg gagtattgaa
     1861 ccgttcagcg cttcgcgcgg cacgcgaacg cacttgccag tcaggctgcg cagcgtgtcg
     1921 tcgctcaccg cctcgtcggg ctgcatccga tcgaccgaaa caagctccac gagccggcac
     1981 acgtagcgat cgcccccggg ttcatatctc aacacgtcga tccgataggt ggcccggaca
     2041 ggacccgaag ccgccgacca cgcgaaacgg atcgtgttcc cccgttcgat gctctcgcct
     2101 ttagccaccc gtcactcgcc cgctgtacga gcgacctccg cgtcgcacat cggcagcagc
     2161 ttgtccagca cttcgagcgc gcggatcagg tgacgctgct cgatgttcag tggcggcgcg
     2221 aggatgatca ggttgccccg cacggcgaaa gatacgccgt tcttgcggcc ctcgtcgacc
     2281 aggcggcgca gcgacggcgc ggtttgcggc cacggcgaca gcggccctct cgtctggcgg
     2341 ttcctcacca gctcgatcac ggcgaacagt ccctgcccgc gaatgtcgcc gatcgaggga
     2401 tggctctctt ccagcacttg cagcacggaa tgcatgtgcg cgccgagatc gcgtgatcgc
     2461 gcgatcaggt cttcgtcacg gtatgcatcc agcgcggcga gtccggcggc gcaggccagc
     2521 ggatggccgc tgtacgtcat gcccgagtac agcatctggc tttcaaactg ctcggcgacc
     2581 ttcctggaca agacaaccgc gccgagaggg acgtgcgcgc cggtcagtcc tttggcgagc
     2641 gtcatcaggt ccggccgtcc ttcctcgcca tactcttgcc aggcgaacca atgtccgacc
     2701 cgtccgaacc cgctcatcac ttcgtcggcg atgaggagta tgttgtagcg agagcaaatc
     2761 tcgcgaaggc gcggccagta ttcaggcggc gcgacgacgc cgttcgtgcc tgcgtgcggc
     2821 tccatcagca cagccgatac ggtcttcggc ccttcccact cgatcagatc ggcgacgtgg
     2881 tcggcgcagc ggataccgca cttgtcatac gcgaggccga gtgggcagcg atagcaatac
     2941 ggcgggacgg cgtggacgac attcggcgtg cccggcgcgt acacccagcc ccgggcgtcg
     3001 ccggagaatt ccatcgcgcc cagcgtcgcg ccgtgatacg agcgataacg cgtgatgatc
     3061 ttgccgaccg gcttattcgt cgcccagcgc gcgagcttga cggcgttctc gttagcgtcc
     3121 gcgccggcca gcgtgaagaa gacacgcccg ccatcgtagc cggacagctc gatcaggcgc
     3181 gcggcaagct ccgcgcgcgg ctttgcgccc caagctgatg tggcgaaaca cagctcgccg
     3241 gcctgccggc ggatggccgc gacgacacgc gggtgctggt ggccgaggtt ggagcactcg
     3301 gccatgctgc tcatgtcgat gtagacgttg tcctgatcat cccaaaagag cgcgccttcc
     3361 ccgccaacga gggtcggtgc gccccagtcg gcctgaaccg tccaggtgtg cattacgacg
     3421 gcgcgctcat cgacggttcg atcggcatcg cctgggtagg gcgaagaagt gggtctactc
     3481 atcgcctgat attgtatgac cgcaggcgga gatggtcaag caacggatag gctcctagac
     3541 cggcggccag ggaacgtgac gctcgtagtg gctgggactg ggaaagcgcg cctgcgcgat
     3601 gagctggtcg gcgcggcggc gcagcgcctc tacctcttcc cgcgccagca ggtcgccgag
     3661 tgacttcgtc accggatccg agcgggaatc aagccgccgg cggaatgcgc gcaaatcgcg
     3721 cagcaggtca gccgggatct tctgcccgcc aaactcccat atgaccgttc gcagcttagg
     3781 ctcttcgtga aaacagatcc cgtggtcgat cgcccagatg tggtcgtccc tgtcgcgcag
     3841 gcagtgacca gccttccgat cggcattgtt gaccagcgcg tcgaagagca cgatcttctt
     3901 gaactgctcg ggaaactgtt tgccgaacgt gaagtaatgc tcgtcgggat cggcgtggat
     3961 gtagaattgc accatgccag gaccgtgcgg tccgtcgcgc aggacagtag gcggaaccag
     4021 catccagccc aaggcctcgc tgacgaggtg cgcggccacc tcgcggtagg ccagagtgcc
     4081 cgccggaaaa tcccacagcg ggcgctcacc tttgcacggc ttgtagacgg ccggcacttg
     4141 cagctcgccg tggacgacga gagtgaggaa cgtatagttg ctgctccacg gcatcagccc
     4201 ctgcacttcg atttcgccgt gtgcgaggag attcagcgca gcgccgaggg aaatgggttg
     4261 tccgtcgtca gagacaggat cggccatgtc ggctcttctc gatcatggcg tcacgagcgt
     4321 gcggctgtgg ccgttgcgtc tcgggcagaa atgcccgtcg gggtcctctg gtcggccaca
     4381 cagcgggcat tgagggcggc cgcgcgcggc gatctccagc gcgtggtcgg ccagcgcgcg
     4441 catttgtgag cgcgttgccc agaagcgggc gacggatgcc gcgcgaggct ctgccgcagc
     4501 gccgggttcc gattctgtgt cttcaggcac caactcttcg gcgaccagca cgaccaggtc
     4561 attttgggca tcgtaaccca accccagcgt cgccacgcgg aagttcggct cgaatggccc
     4621 acgcaactcc agatcgaggg ggccgactga atcgccttcc agttccgccg gaaacttctc
     4681 atcgatctcc ttgaggaact ggtagacgcc gcgcgccagc atcacgacct gttccttttc
     4741 ggcgatgagc gtgacgattt cgtccagcgc gcgagcctgg atgtaaaaca cgcggtggcc
     4801 cggctcgccg accgccccgg ccgtaatggc ctttaccgga ttaagctcgt acaccctctg
     4861 agacatcacc ctctctttat cgttggcgag cacgcccgtt ttgcgatggg ggcgcgaacg
     4921 gtccagtatc gttgaggcgc agcagccgcg cgccaaagcg tcccatgccc aggacggtta
     4981 ccgatgcggg cgcgatgatc agcctctgga acgaatcgag cggcatgccg acgaagtgcg
     5041 cgacgatcgc cttgatcacg tcgccgtgcg agaaggcggc tacgttgccc ttgggatgag
     5101 cggcgacgat gcgctcgatg ccggcgacgg cgcggctctg catctcgcgc agcgactcgc
     5161 cgttcgggaa gcgcatcaca ctaggcgcca tctgcacgat cgcccattct tttctttttt
     5221 tgagctggcc gagtgacttg ccttgccagt ctccgtagcg cacttctccg agatcgcgca
     5281 tccggatcac tttcaggccg cgcgctcgcg ccaggggcac agccgtttcc agcgtgcgct
     5341 ccaaagggct gctgtatatc gcctcaagct cgaggggcgc caaccgctcg accagcgcct
     5401 cggcttgtcg ccggccggcg tcgttgagat gcacgccggg cgtccagccg gcaagtttct
     5461 tatcggccca atcgttgtgg ccatgtcgga tgagaaagac ttgggtcatg tggcttgaag
     5521 actgaataca tcgattcgcc taaaaagtgt accacgattc agcgccggat gacaaccggg
     5581 gtatcgatcg tgcatgcgcc gtcaattgct gtgccggatg tgaacgatgt ctccgtcctc
     5641 cacaacgtgt tctttgcctt ccagtcgcag cctgccgctg gctttggcct cgttcatgct
     5701 gcccagctcg accagatcgt gatacgagac gacctcggcg cgaatgaagc cctgggccag
     5761 gtcggtgtgc acggtcgccg ctgcctcgag cgccgtggcg ttcttgcgca ccgtccaggc
     5821 gcgcacctcg tcctcgccga tggtgaagaa cgattgcacg ccgagcaagt cgtatgacag
     5881 ccggatcact tttgttcggc tcagctcgga gatgccgtat tcctgcatga acagcgcggc
     5941 gtcgttggcg gacagctcga tgatctcgtg ctcgagccgg gcgttcagcg cgacgacagc
     6001 ggtcttagcg gaatcggctt cgaaatcggg cgcgcggccg ccttcgccga tgttgaacac
     6061 caggagcatc ggcttgagcg agagcaggcc gaaaccgcgc aggcgctgct cctcttcggc
     6121 gctcagcgcg acggcgcgca gcggtgtctc cgcctcgagc gtctccttga gccgcgcgag
     6181 cagcgccttc tccttggcag cctgctcgcg atcgggcgcg cctttcttga tgctctgttc
     6241 gacgcgctcc catcgccttt cgacaatgcc cagatcgttc agaagaaact cagtgtcgag
     6301 gatcgccctg tcgcgcagcg gatcgaccga gccttcactg tgcggcacac tcggatcatc
     6361 gaacgcgcgc aggacgtgga cgaacgcgtc catctggccg atttggttca cgaacgggcc
     6421 gggcaatccg gtcttgccga cgcctttgtc gaggccaccg acgtcggcgt acatgacctt
     6481 cgcgtagacg ggcttgcgcg gcttgaacat cgcgctgagg atgtcgacac gcgcgtcggg
     6541 cacagaggcc acggcggtct gaatcttgag agggccagcg gagaacgtcg cgacggcttt
     6601 ctccccgcct gccagcgcgt ggaatagcgt ggtcttgccg catcgaggga gtccgataat
     6661 gccgatttgc atgtatcgga ttatcctcta tgcacgttca ctcgtcaagg cggcgcatct
     6721 ggtgattgcc cgcacttgct ttattgggcg gcaacagtat actatacgct accacggtaa
     6781 gcgccggtca atatgccggc atggggcgtg cacgcaatac gtgtcaggct gctcaccgcc
     6841 cgcgcctctg gaggcggcca accgcgtcct cgcggttggc gagccggctt ctctctgaca
     6901 acctttacgt gcacacggca gaggtggcat atgcgtcaac tgatgatagt gtgcctgctg
     6961 gttacagtac tgtccttcgc atcgactttg acagcggtcg ctcagttcgg agggcctaac
     7021 ccggtactga ccaagacgat gactccgtct cctcccgccg tcgggaagcc ggcgcgtatc
     7081 acgattacgg cgacgaatgc cggaacggct aacgccgaca acgtcgtgat caccgatccg
     7141 gtgcccgaca acatggccct cgccggcgtc acggcgtcgc agggcacggt cgtcgtttcg
     7201 cgcggcatcg tcaccgtata cgtcggcacg cttgcgccgg gccagacggt gaccgttgtc
     7261 agcgatgtca tcattacccg cgcgttcgcg aacgacacgc cgtacacgaa ctgcacgggc
     7321 ctgacttttc gagacggcac ggccagattg gcgtgcttcc cgttcggacc ggcattcggg
     7381 ccggtgtccg tcgcgtctcc gccgcggttc ttgcctgacg ccggcgcgcc aggcggatgg
     7441 ctgccgctca gcctgattct catcggctcc gccggtctac tggtcgggct gcgcctacgg
     7501 ctgagacgtt gagccatgcc cgatcgaccg accctcctca tcgtcgaaga cgacgacggg
     7561 ctgcgcgtct tgctgcagac tgctctgggc cgcgacgact acgacgtcac ggcggccggg
     7621 agcggagaag aagcgctgcg caagatgtca atcctggcgt tcgacgtggt gatcacggac
     7681 atccgcctgc ccggcatcga cggcgtgcag ttggtgcgcg atattcgccg ccgttggccg
     7741 gacatcgtcg tcatcgtcct caccggctat cctgcgctgg agagcgccgt cgcctgtctg
     7801 agggccggcg ttcacgatta cctctacaag ccgtgcccga tcggggagat ccggcgcagc
     7861 ctgcgcgagg gtctggccaa gcggcatgga ttcgccaggc ggctcgagtt gctgcaaggg
     7921 ctggagcagc agctcacaca agggttgcgc gtcttgcgcg gcgagatgcc cgccaccagc
     7981 tcaatgctcg acgcgatggg cgacggcgcg acgacggttg ggcgatacgt gcgcaccggc
     8041 tcgttgatca tcgaccgcga gcggcacgaa gccatcctgg gcgagacgca gctcgacctc
     8101 acgccgaccg agttcgagat actggggtat ctggccgagc gcgcgccggc cgtcgtgacg
     8161 ccggtcgacc tggcgcggcg cgcgatgggt tacgacgccg acgagcaaga agcgcgcgag
     8221 ctggtcaagt ggcacatcca tcatttgcgg cagaagatcg aggccgatgc gacgcggccc
     8281 atcttgttga aaaacgtgag aggcgtcggc tacaagctgg acgtggcgtg acgcctgtta
     8341 agagacgatc actccttgcg
//
LOCUS       DBNHXB010000273         3727 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1747663, whole genome shotgun sequence.
ACCESSION   DBNHXB010000273 DBNHXB010000000
VERSION     DBNHXB010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1747663"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="AC162G_11060"
     CDS             <1..1334
                     /locus_tag="AC162G_11060"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR5076402.1"
                     /translation="FRDRGEFQRAIEVYEDVFNMASEIGDQSLVSLVLDGLGHAHRLS
                     DGLDRAVSLFEQARSVASRGGIDSQAILSEASISMGRFEQGGVSPGIQGLEHAERALR
                     ESGSHLDLARVLFWLARAYFEAGRHAEARESLAELMRLAGRLGYRPFSSAEGRRAEAF
                     LQWAASLTDADSRLRLWIGELRAARTAAAPGVQVEAIAAPRIEVRAFGSGQVVRAGRL
                     LAASEWGGSAAARELFFYLLEKSPQRKEEIGAAFWPDLSPARMTNAFHAVKYRIRRAL
                     DVEFVIYEDDRYRVNPAVDYGYDIAEFERLLDSAARRPANDPGRYALLDEALALYSGD
                     YLTGIYSEWALDKRRALQAQFFEALDQLLPELLRREEYERAAELSRRGIEVDTFREEL
                     HRSLMWSLARTGRRAEALAHYKTFAQHMRKELRVSPSQETRDLYLRIRAQR"
     gene            1770..1904
                     /locus_tag="AC162G_11065"
     CDS             1770..1904
                     /locus_tag="AC162G_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076403.1"
                     /translation="MLGIIRKNVASIANHPIVVQVAAAAMALTVLALPMAPGAGGGGA
                     "
     gene            1922..2155
                     /locus_tag="AC162G_11070"
     CDS             1922..2155
                     /locus_tag="AC162G_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076404.1"
                     /translation="MNYPVLEEVVGQAIIDRDFCAGLLNGRRARLLSRFSLTPEETQV
                     LMSIRADSLEAFAGQLYRWIESRQPARRRVHLA"
     gene            2171..2578
                     /locus_tag="AC162G_11075"
     CDS             2171..2578
                     /locus_tag="AC162G_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5076405.1"
                     /translation="MTLRVLIADDDPDLREIVRLTLQRDGYEVIEAGDSEQTIARAID
                     SAPSLILLDLMMPGADGFDTCRQLKNDERTVAVPVIFITGQGDPRARTRGLQLGAADC
                     LDKPFHPRDLSSRVRMAIQRRGINTLFGAVAPS"
     gene            2755..>3727
                     /locus_tag="AC162G_11080"
     CDS             2755..>3727
                     /locus_tag="AC162G_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076406.1"
                     /translation="MTHRAHVLNGLVRVLIVAGLFLSPAGFVQTRTASAQTPTPTSSP
                     PPEPTATPTAAPPSDATLTSTPEPTATPSATPDPSPSSGQAPSATLEASPSATPEASP
                     TPTETAVSPTPTLEAEDVVTVTLDAAGGVVTSPDGRARLEFPPDALSGPAAIVASVLR
                     PERDGRGRVRMTRFRLNAYALDAQGRIGEPLTRFARPYTLTVDVRGVLDVQALPPNER
                     LALYYAPDPSPGSGQATAPDEWIEIARTQQPQPGVLAASLDHFTAFAAGATASPSSGW
                     KLVYHPPVPDLFSGAATYNYPIDVPPGRNGLQPRVNLAYSSRGLDGLV"
BASE COUNT          613 a         1213 c         1228 g          673 t
ORIGIN      
        1 tcttccgcga ccgtggagag tttcagcgag cgattgaggt ctacgaagat gtattcaata
       61 tggccagtga gatcggagat cagtcgttgg tcagcctggt gctcgatggt ctcggacacg
      121 cgcatcgcct gtcggatggg ctggaccgag ccgtgtcgtt attcgagcag gcacgcagtg
      181 tcgcgtctcg aggagggatc gattcacagg ccatactttc ggaagcgtcg atcagcatgg
      241 gacgtttcga gcaaggaggc gtctcgcccg gcatacaagg gttggagcac gccgagcgtg
      301 cgctgcgcga gtcaggctcg catctcgacc tggcgcgcgt gctgttttgg ctggcccgcg
      361 cctatttcga ggccggccgg cacgccgagg cgcgggagag cctcgccgag ctgatgcgcc
      421 tggcggggcg gttgggttat cggccgttct cttcggccga aggccggcgc gccgaggctt
      481 tcttgcagtg ggcggcatct ctaacggacg ccgacagccg gctgcggttg tggatcggag
      541 agctgcgcgc ggctcgcacg gctgccgcgc ccggcgtgca ggttgaggcg atcgccgcgc
      601 ctcgcatcga ggtgcgggcg ttcggttcgg gccaggtcgt tcgggctggg cggctgctcg
      661 ccgcctccga gtggggcggc tcggctgccg cgcgcgagct tttcttctac ctgctcgaga
      721 agtcgcccca gcgcaaggaa gagatcggcg cggcattctg gccggacctg tcgccggcca
      781 ggatgacgaa cgcctttcac gcggtcaagt accggattcg gcgcgcgctg gatgtggagt
      841 tcgtcatcta cgaggacgat cgttatcgcg tcaatccggc cgtcgactac gggtacgaca
      901 tcgccgagtt cgagcgtctt ctcgattcgg ctgcacgccg gccagcgaac gacccggggc
      961 gctacgcgct gctcgacgag gcgctggcgt tgtacagcgg agactacctg accggcatct
     1021 actcggagtg ggcgctcgac aagcggcgcg ccctgcaggc gcagtttttc gaggcgctgg
     1081 atcagctcct gcctgagctg ctgcgacgcg aggaatacga gcgggccgcg gagctgagcc
     1141 ggcgggggat cgaagtggac acctttcgag aggagcttca ccggagcctc atgtggagcc
     1201 tggcgcgaac ggggaggcgg gcggaagcgc tcgcgcatta caagacattc gcgcagcaca
     1261 tgcggaagga gctacgggtg tcgccgtcac aggaaacccg ggacctgtac ttgcgtatcc
     1321 gcgcgcagcg ctaggccgtt ctccgcgccc actctcggcc ggttgtcttc ctctatcgtg
     1381 ttaatcttac cgcgagcgcg cacacttgcc cctgtgctgc cgcacgcgtg catttcactt
     1441 ttggggcgac ccttcgagca gtgtcagaaa ttctagcggt gcgctgttcc gatggcagtt
     1501 gcactgccgt cgccgcagtc caactgtggc gaaaacacga ggccatgcgg cacgtggcct
     1561 caggtgatcg cgtttcaccg ggggcttttg caaattttca ggcaattttc ctacgccaca
     1621 tgtgggtatg atagttctac gagacgccat atatggcgca gaagaggcca aaaacaggcc
     1681 aagactgcgc gcagggggtc ttcgactgtc ataggtactt acatatcctt cttgtagaat
     1741 gccgcacaaa tcgacaaagg gggcaaatca tgttgggaat cattcgcaag aacgtcgcct
     1801 cgatcgccaa tcatcctatc gtcgtgcaag ttgccgcggc tgccatggcc ctgacggttc
     1861 tcgctctacc catggcgcca ggcgctggcg gcggcggcgc atagtttagg gagatgtcac
     1921 tgtgaactat cccgttctcg aagaagtcgt cggccaggcc atcatcgatc gcgacttttg
     1981 cgccgggctt ctgaacggca ggcgcgcccg gctgctgagc cggttcagcc tgacgcccga
     2041 ggaaacgcag gtgctcatga gcatccgcgc ggattcgctc gaggcgttcg ccggccagct
     2101 ttatcgctgg atcgagtccc ggcagcctgc gcgccgccga gtgcacctgg cgtgaccggc
     2161 cggagctacc atgacgctgc gcgtcctcat cgccgacgac gatcctgatc tgcgcgagat
     2221 cgtccgcctg accttgcagc gggacggata cgaggtgatc gaggcgggcg atagcgagca
     2281 gactatcgcg cgcgccatcg attccgcccc gtcgctgatc ctgctggacc tcatgatgcc
     2341 cggcgccgac ggattcgata cgtgtcgcca gctcaagaac gacgagcgca cggttgcggt
     2401 gccggttatt ttcatcaccg gccagggcga tccgcgcgcg cgcacgcgag gcttgcagct
     2461 cggcgccgcc gactgcctgg acaagccatt ccacccgcgc gacctcagct cgcgcgtgcg
     2521 catggcgatc cagcgccggg ggatcaatac tctcttcggc gccgtcgccc cgagctgatc
     2581 ccttctgcat tttgaagcag agcaggctcc cacgcctgcg aatcggcgac ataggtgttg
     2641 aactctctct cgcttcggac ttagccgtgc ggacataacc gaatacttcc ccgtgcgcct
     2701 ctcggagacg cggactttga actgccgtta agaaacatag gcaagggggc aaccatgacg
     2761 caccgtgctc atgtgctgaa tggtctggtc cgggttctga tagtggcagg cctgtttctg
     2821 agcccggccg gatttgtgca gacgagaacg gcgtctgctc agacgccgac cccgacgtct
     2881 tctccgcctc ccgagccgac cgcaactccc acagcagctc cgccatccga cgccactctc
     2941 acctcgacac ctgagccaac cgctacacct tcggcgacgc ccgatccttc gcccagctca
     3001 gggcaggctc cttcggctac gctcgaggca agcccgagcg cgacgccgga agcatctccg
     3061 actccgaccg agacagcggt atcgccgacg cccacgctgg aggccgagga tgtcgtgacc
     3121 gtcaccctcg acgcagcggg cggcgtggtg acctcacccg acgggcgggc gcggctggag
     3181 ttcccgcccg acgcgctgag cggcccggcg gcgatcgtcg cctcggtgct gcggcccgag
     3241 cgcgacggca ggggccgtgt gcggatgacg cgcttccgcc tgaacgccta cgcgctggac
     3301 gcgcaaggcc gtatcggcga gccgctcacg cgcttcgccc gtccgtacac gctcaccgtc
     3361 gacgtgcgcg gcgtgctgga tgtccaggcg ctgccaccca acgaacggct cgcgctgtat
     3421 tacgcgcccg acccttcgcc cggctcaggg caggctactg cgccggacga gtggatcgaa
     3481 atcgcgcgga cgcagcagcc acagccgggc gtgctggccg cgtcgctcga ccacttcacc
     3541 gcgttcgcag ccggcgcgac ggccagtccc agctcgggtt ggaagctggt gtatcacccg
     3601 ccggtgcccg acctgttcag cggtgcggcg acgtacaact accccatcga cgtgccgccg
     3661 ggccgcaacg gcttgcagcc cagggtgaac ctggcctaca gcagccgggg gctggacggt
     3721 ttggtgg
//
LOCUS       DBNHXB010000274         3583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1077780, whole genome shotgun sequence.
ACCESSION   DBNHXB010000274 DBNHXB010000000
VERSION     DBNHXB010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1077780"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="AC162G_11085"
     CDS             complement(<1..140)
                     /locus_tag="AC162G_11085"
                     /inference="COORDINATES: protein motif:HMM:NF016167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR5076407.1"
                     /translation="MAVVLDRYIEIKADVRSGKPCVAGTRITVSDIVLMHFRLGQSLE
                     EIA"
     gene            complement(170..1915)
                     /gene="recN"
                     /locus_tag="AC162G_11090"
     CDS             complement(170..1915)
                     /gene="recN"
                     /locus_tag="AC162G_11090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR5076408.1"
                     /translation="MLAELLITDFAIIDELRLKLAPGFNVLTGETGAGKSIIIDAVGT
                     LLGGRVETQMVRAGTARAAIEGMFYLDDATRAAIDPILKENDLVDDEEPDMLLLSREI
                     RAGGRSTSRVNGRVVTTAILKSIGEVLVDIHGQSEHLSLLRPKEHVNLLDRYAETWEA
                     RQRLASVVAQWRSVRKELDTLLRSEAELARRADLLKFQVEEIGDARLKEGEEEALTAE
                     RNRLANAETLASLADEAYAALYEGAGETPAALDALAQARKAIAGLAKIDPSLARHSET
                     IESVNAQIDDLAREISDYRDRIEYSPQRLEFVEERLDLIKRLKRKYAPNGSIADVLAF
                     AQKAAEELDTIEHSGERVEELRDEEERLLREIGLLGKQLSEARRAAADRLSAGIEAEL
                     NDLRMSGARFAVDMKWEAAAEGAFVDDGRFAFDMTGLDKIEFLIAPNIGEGLKPLAKI
                     ASGGETSRLMLALKNVLSRADQTPTLIFDEIDQGIGGRVGVTVGQKLWGLSGNHQVVC
                     ITHLPQLAGYGDAHFKVEKIVDGERTVTRVGLLEGETRIEELAQMLGASGEGATLSAQ
                     EILAGVAEVKTKVMM"
     gene            complement(1926..2453)
                     /locus_tag="AC162G_11095"
     CDS             complement(1926..2453)
                     /locus_tag="AC162G_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076409.1"
                     /translation="MSEQATSSAGETLYCYYHPNTPTTLRCNRCNKPICSKDAVRTAV
                     GYRCKTCVRQQQDVFFNATQADYVIAAIAALPIGYLAQRFVPQLGFFVIFLGPVIGGL
                     AGEAIWRVTGRRRGRYVWALALASLTAGALAALWPQLQLFFLFGAQALTRILWDAVFF
                     VLMAGAVLARLRLWR"
     gene            complement(2480..3382)
                     /locus_tag="AC162G_11100"
     CDS             complement(2480..3382)
                     /locus_tag="AC162G_11100"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039482.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR5076410.1"
                     /translation="MAYVTPVNTPARCAPPRRVVVAHTPQINASSRLAGQIAEQLAQW
                     KVQAQLASLEDEDIDVRLDGIDMVIALGGDGTMLRVGCLTAPRNIPVLGVNLGRLGFL
                     VEVQPSEWPGVLARVMAGDYWLEERLMLHADVERDGEVVQSLEVLNEVFVGRGATGRP
                     VRIEVCIDGGLLANYLADGLIVATPTGSTAYALAAGGPILPPELKNILLVAVAPHLVI
                     DRPVVLHEGTTVDLRVATTRPAMLSADGRDPMPLANGDRVRVQSSPYVCRFVRVQPKS
                     YFYRTLMARMAQNPAADLEKNPND"
BASE COUNT          595 a         1250 c         1090 g          648 t
ORIGIN      
        1 gcgatctcct ctaaagactg ccccagacga aagtgcatca acacgatgtc ggaaacggtg
       61 atacgtgtgc ctgcaacaca cggcttgccg cttcgtacat cggctttgat ttcaatgtat
      121 cgatccagaa cgactgccat ttgaacctgc ctcctacacc cgacactatt tacatcatca
      181 ccttcgtctt cacctcggcc acgccggcca ggatctcttg cgcgctgagc gtagcgccct
      241 ctccgctcgc gccaagcatt tgggccaatt cttcgatgcg cgtctcgccc tccaacaggc
      301 cgacccgcgt gacggtgcgc tcgccgtcga cgatcttttc gaccttgaag tgcgcgtcgc
      361 cgtagccggc taactgcggc aggtgcgtga tgcacacgac ctgatgattg ccgctcagcc
      421 cccacagctt ctggccgacc gtcacgccga cgcgcccgcc gatgccctga tcgatctcgt
      481 cgaagatcag cgtcggcgtc tggtcggcgc gcgacaacac gttcttgagc gcgagcatca
      541 ggcgagacgt ctcgccgccc gacgcgatct tcgccagcgg cttcaatccc tcgccgatat
      601 tcggcgcgat gaggaattcg atcttgtcca gccctgtcat gtcgaacgcg aagcggccgt
      661 cgtcgacgaa cgcgccctcc gccgccgcct cccacttcat atcgacggcg aagcgcgcgc
      721 cggacatgcg caggtcgttc aattcggctt cgatgcccgc cgagagtctg tccgccgctg
      781 cgcgccgcgc ctccgacaat tgcttgccca acagcccgat ctcgcgcagc aggcgctctt
      841 cctcgtcgcg cagttcctcc acgcgctctc cggaatgctc gatggtgtcc agctcctcgg
      901 cggctttttg ggcaaacgcc agaacgtcgg caatcgagcc gttgggcgcg tacttgcgct
      961 tcaggcgctt gatcaggtcg agccgctcct ccacgaactc cagacgctgg ggggagtact
     1021 cgatgcgatc gcggtagtcg ctgatctcgc gcgccaggtc gtcgatctgc gcgttgaccg
     1081 actcgatcgt ttccgaatgc ctggccaggc tcggatcgat cttcgccagc ccggcgatcg
     1141 ccttccgcgc ctgcgccagc gcgtcgagtg cggcaggcgt ctcgcccgcg ccttcgtaca
     1201 gcgcggcgta cgcttcgtcg gccagcgagg cgagcgtctc ggcgttcgcc agccgattgc
     1261 gctcggcggt caacgcttcc tcttctccct ccttcaagcg cgcgtcgccg atctcttcca
     1321 cctggaactt gagcaggtcg gcccggcgcg ccagctcggc ctcgctgcgc agcagggtgt
     1381 cgagttcctt ccgcacactg cgccactgcg cgacgaccga cgcgagccgc tgccgcgcct
     1441 cccacgtctc ggcatatcga tccaggagat tcacgtgctc tttgggacgc agcagcgaca
     1501 ggtgctcgct ctggccgtga atgtcgacga gaacctcgcc gatcgatttg aggatagcgg
     1561 tcgtgaccac ccgcccgttg acacggctcg tcgagcggcc accggcgcga atctcgcgcg
     1621 agagcaggag catatccggc tcctcgtcgt cgaccagatc gttttccttg aggatggggt
     1681 cgatggccgc gcgcgtcgca tcgtccaggt aaaacattcc ctcgatcgcg gcacgcgccg
     1741 tgcccgcccg caccatttgc gtttcgacgc gcccaccgag cagcgtgccg accgcgtcga
     1801 tgatgatcga cttgcccgcg ccggtctcgc cggtcagcac gttgaacccg ggagcgagct
     1861 tcaggcgcag ctcgtcgatg atcgcgaaat cggtaatcag gagttcggcc agcattgtca
     1921 atcccttatc gccacaggcg cagccgcgcc agaaccgcgc cggccatcag cacgaagaac
     1981 acggcatccc agaggattct cgtgagcgcc tgcgcgccga acaagaagaa caactgcagt
     2041 tggggccaca gagcggcgag cgcaccggcg gtcaggctcg ccagcgcgag ggcccagacg
     2101 tagcgcccgc gccgccggcc cgtcacgcgc cagatcgcct ctccggccaa tccgccgatc
     2161 accgggccga ggaagatgac aaagaacccg agctgcggca cgaagcgctg cgcgagatag
     2221 ccgatcggca gcgccgcgat cgcagcgatt acgtagtctg cctgggtcgc attgaagaac
     2281 acatcctgct gttggcggac gcacgtcttg cagcgatagc ccacggccgt gcgcacggcg
     2341 tctttggagc agatcggctt gttgcagcgg ttgcagcgca gcgtcgtcgg cgtgttggga
     2401 tggtagtaac agtacagggt ttcgccagcg ctcgaagtcg cttgctctga catgtcactc
     2461 tttccggaca aattcaacgt tagtcgttcg gattcttctc caggtcggcg gccgggtttt
     2521 gcgccatgcg cgccatcaac gttcgataaa agtacgactt gggctgcacg cgcacgaagc
     2581 ggcacacgta cgggctggac tgcacgcgga cgcgatcgcc gttggccagc ggcatcgggt
     2641 cgcgcccgtc ggcgctgagc atggccgggc gcgtcgtcgc cacacgcagg tctaccgtcg
     2701 tcccttcgtg cagcacgacc ggccggtcga tgaccaggtg gggtgcgacg gccacgagca
     2761 agatgttttt cagctccggt gggaggatcg gcccgccggc tgccagcgcg tacgccgtgc
     2821 tgccggtcgg cgtggcgacg atcaagccgt cggccagata attcgccagc agcccgccgt
     2881 cgatgcacac ctcgatgcgc accggccggc ccgtcgctcc gcgcccgacg aacacctcgt
     2941 tgagcacttc cagcgattgc accacctcgc cgtcacgctc gacgtcggcg tgcagcatca
     3001 ggcgctcttc cagccagtaa tcgcctgcca tcacccgcgc gagcacgccc ggccattcgc
     3061 tgggctgcac ttcgaccagg aaccccaatc gtcccagatt cacgcccagc acggggatgt
     3121 tgcgcggcgc ggtcaggcag ccgacgcgaa gcatcgtccc gtcgccgccc agcgcgatga
     3181 ccatgtcgat gccgtccagc cgcacgtcga tgtcttcgtc ttcgagcgag gccaactgcg
     3241 cctgaacctt ccactgggcc aactgttcgg caatctgccc ggcgagcctg gacgatgcgt
     3301 tgatctgcgg cgtgtgcgcc accactaccc gcctgggggg ggcgcagcgc gccggggtat
     3361 tgaccggagt cacgtatgcc atatcctgaa tcgtactgac cggcattata acccgattca
     3421 agccaggcgc tcgtcgcacg cgtggcggta accgcagttg gcgcagcgcc ggggatccga
     3481 gtgcgatcgg tgcgcctcgt tttcgctcgc agccaggcgc atttcgtcaa gcatgtcgag
     3541 cagcgacgag cgcagccggt cagtgtgctc gatcgagagc gtc
//
LOCUS       DBNHXB010000275        11269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1414551, whole genome shotgun sequence.
ACCESSION   DBNHXB010000275 DBNHXB010000000
VERSION     DBNHXB010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1414551"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..761)
                     /locus_tag="AC162G_11105"
     CDS             complement(69..761)
                     /locus_tag="AC162G_11105"
                     /inference="COORDINATES: protein motif:HMM:NF053467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FFLEELY motif protein"
                     /protein_id="HIR5076411.1"
                     /translation="MLHDFQQLKRHPVEGQPLDPKIALLRTWQSERLARTYADLVANP
                     RYQPAYLFFLNEVYAPRDFSQRDRDMETVHDALQHILPDALIRPLALTVELHHLTEAL
                     DQRLIDVLTQQLGVTDTITTALYAEAYRICDNYDERVRQIDLVHQIGSRLDGVVRSPL
                     TRAMLAVSKGPARKAGWGELTDFLENGYRVFKHMRGARHFLDTVRERERRILDRIYAN
                     DPDPFGFDESVS"
     gene            complement(768..1109)
                     /locus_tag="AC162G_11110"
     CDS             complement(768..1109)
                     /locus_tag="AC162G_11110"
                     /inference="COORDINATES: protein motif:HMM:NF023178.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HIR5076412.1"
                     /translation="MLTPFERGFVAHLFADWLLQNDWMARNKISLRHPAAWVHGAIHA
                     LLLGLALGWPAGLALGIVHMLIDTRLPLSWWQRVYHQTGDGPYARHVAIWADQVLHIV
                     FVAAWVALSGM"
     gene            complement(1134..4802)
                     /locus_tag="AC162G_11115"
     CDS             complement(1134..4802)
                     /locus_tag="AC162G_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5076413.1"
                     /translation="METLIAYIPADRLHALADGETLPDRTRGAALFADISGFTPLTEA
                     LARELGPQRGAEELTRHLNLVYDAIIGELNRYGGSVISFSGDAITCWFDGDSGLFATA
                     CALSMQQTMKRFTSVALPSGQTLALAMKVAVSTGPARRFLVGDPSIQLIDALAGAILD
                     QLAVAEHQAGKGEVVLDPAAVAALADQVHIGEWRVGENGQRFGVVSGIVKPVPPLAWA
                     PIRADALRDEQVRPWLLPAVYERLSSGQGEFLAELRPAVALFLLFGGIDYDADEEAQT
                     KLDAYIRAVQGIVARYEGSLLQLTIGDKGSYLYISFGAPLAHEDDVVRAASAALELHV
                     LKKDFPYIHDARIGISRGRMRTGAYGGSMRRTYGVLGDDVNLAARLMQAAAPGQILAS
                     RTAYQGAEAEFEWERLPDIRVKGKVEPVAIFSLIGSRERRAVRLQEPDYALPMVGRES
                     ELKIASQKIDQARDGRGQILGITGEAGIGKSRLVAEIIRGTGEEQIVGYGGECLSYGT
                     NTSYLVWHSIWRSFFGVDPAWSLEKQVKVLTRQLRLIDDALVPRLPLLGAALGLTIPD
                     NDLTQGFDAQLRKTSLESLLVDCVRGRARMTPTLFVLEDCHWLDPLSHDLLEAVGRAI
                     ADVPVLIVMAYRPPDVERLQALRVSQLPHFTEIRLTSFTPQEAEELIRLKLDRFFGSH
                     VAAPRDVVDRVVARAEGNPFYIEELLNYLQDRGIDPQDSRALEQLDLPASLHSLILAR
                     IDQVTERQKTTLRVASVVGRLFKAAVLWGAYPPLGGPQHVKADLDALSRLELTSLDAE
                     PELTYIFKHIVTQEVAYESLPFATRAMLHEQIAQYIEQSYPQAPEQQVDLLAFHYDRS
                     ENEAKKSEYLLRAGEAAQERYANAAAIDYYRRVLRLLPAAQQVPVLLKMGQVLEVVGR
                     WDESLDAYRQALELARDLGDRQAQARSRAATGELLRKRGRYDEAARWLVQARAGFEEL
                     GDEAGVGQTLHYAGSLAAQQGNTDAARALYGESLAIRRKLEDKPQIASLLSNLGIVAR
                     MRGEYASAHALHEEALAIRRELKDRRAIGVSLNNLGFVALELGDYTEARARLEEAIAL
                     NREVGDKQNTGVALNNLGNVVRSRGDYTTARKHYRESLVLYRELGDRWSLAYLLEDMS
                     VLAALQSQPERALRLAGAASALRDMIGAPHSSVEQEKLEQMLEPARRAMSDDEQETYH
                     AQGRAMSLDQAIEYVLSD"
     gene            complement(4815..5060)
                     /locus_tag="AC162G_11120"
     CDS             complement(4815..5060)
                     /locus_tag="AC162G_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family ATP-binding protein"
                     /protein_id="HIR5076414.1"
                     /translation="MRKLDVPRMALVANKMPQAFDPAEVKARVEKAYNCPVIAVLPHS
                     DDLMALASSGVFVLRFPDHPISAMLRDIVRLVVNEPA"
     gene            complement(5400..11243)
                     /locus_tag="AC162G_11125"
     CDS             complement(5400..11243)
                     /locus_tag="AC162G_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HIR5076415.1"
                     /translation="MNSTADAVNTDGACTLREAIIAANKDQASSNNPGECAAGSSVDT
                     IVLPAGTYTLTRSDNGKEDSSNTGDLDITASVVISPTGPVTITASSGFSDRIFHILGG
                     SATISGVTISSGNVSSDGGGIYNLGTLTLLNGTVSDNKAGGRGGGIYNAGAATLANVT
                     ISGNTAKTDGGGLYNTGSASAATLNNVTVTANTADSDGNGSGNGGGVFRSGGTVGVKN
                     TIIAGNTDNSGATQHPNCSGSLISQGHNLIGPSTGCAGFVDGANGDRVGVADPDLNLG
                     SLQDNGGATFTHALLAGSPAIDAGNPATPGSGGDACSLTDQRGAYRPRGRACDIGAFE
                     AGAQNSPVVIRVVLGNAGTTVDGRLFSAANTTFTLTFYTSPTCDGPKTLLGSSTVTTN
                     DSGNVYFRSVIDALATEGQFVTATATDPNGNVSGSSSCVTVGQGNDAWTRAFSMTLTG
                     TGSTVSASASHYIDLQGQSRWYKFSVQPGSKVIVALTDLPANYDLTVYKDISATLRSL
                     LEINAQQDLSHLGAEFAIDAFSPEIFSPEIFSPEIFSPEIFSPEIFSPEIFSPEIFSP
                     EIFSPEIFSPEIFSPEIFSPDALSPEIFSPEIFSPEIFSPEIFSPEIFSPEIFSPEIF
                     SGAQTRSVIAVSGFDGTAGEGVSVNTWNNTGDFYVRVRGRNGAFSLASPFRLQVVQQL
                     GACSNVSSAGFPPSNLANSAAAVSAADGPYKTAILIDSGRMGITGALMDALDAFAARP
                     EVRGVVLDVSLDDRVTDANDQADERPQCPFAKNLVAESIKGIVDAYRDANPTLEYAVI
                     VGNDNVIPFFRYPDNASLANENNYVPPVRDNTASQASLRLGFVLSQDAYGSRFGLSLK
                     NNSFPIPDLAVGRLVETEADVIQMLDAYMSTTDGVAPTPASSLVTGYDFLADAANAVN
                     AELVAGMGAGASHDTLIDPRNRSPQDADAWTADDLRDQLLDSRHDLIFLAGHFSAGGA
                     LAADYRTRVTADEIVSSSLDWRNVLVFSAGCHAGYNIVNPHGIPGVTADPDWAQAFNG
                     KGASLVAGTGYQYGDTDFVEYSERLYVEFAKQLRAGSDPVAIGQALVQAKQAYLANTP
                     VMRGIHEKSLLEATLFGLPMLKVDLPSGRGDAPTDGSIVPGTSGFAADPGQTLGLRFA
                     DVNIPMSLTPNTVPLSNTANLAQTVTAQYLSGSSGTVSNPGEPVLPLESRDVSVPGLA
                     LRGVGFRGGSFTDLPDILPFLSAATTELRAAHAPFLSDVFYPIRPWNVNYFDALSGGA
                     GVTRLDVIPAQVQSGAPGASTVTLRQFDDMSFRLYYSSHTASYSNGNVPALAAAPSIV
                     RVLGIPGDGNVTFHVNVVGSPGAGMQEAWITFTALSGPYAGRWQSLDLAQSLTDSTLW
                     TATLPLAGTPAGDIRYIVQAVNGVGLVGLDTNKGAYYIPGNVTEPTEPAVLEFQSAPS
                     SGTYGARATFSAKLTSDGMPLGGQRVVFRLGPQSRLAFTDNTGVATATLSLLGLPGQY
                     EVRASFAGTSIYLASSATAPFEILHQDTILTLDPTSATVPRGAGTPITATLTDAGGRR
                     LPEKTVFFVVTGSGGSHTVAVITDYLGRAPLGGVPAPVGVYAVNAYFSGVIPLPDGTL
                     ALVNDRYISSTATGSLTIFNRAPVAGNDAYSANEDATLFVAAPGVLSNDSDPDGDSLS
                     AILVFGPLSGTLALNADGSFTYTPNANFNGGDSFTYKANDGFDDSSVATVSITVHPVN
                     DNPVAGNDAYSVNEDTSLNVPAPGVLGNDSDIDGDGLTAVLVNGPAHGALTLNANGSF
                     VYTPDANFHGGDSFTYKANDGAADSNVATVVITVHSVNGVPVCTDAVASTVTLWPPDK
                     VFHPVTVLGVTDPDGDMVTITITGIRQDEPTGTTADGMGLGTSTAYVRAERDGNGDGR
                     VYHIFFTASDGRGSCDGQVRVGIMPHDQSGTIGDVDQGPLYDSTVAQ"
BASE COUNT         1934 a         3626 c         3642 g         2067 t
ORIGIN      
        1 caagtccgca atatcgtgca agacatgatc tacgcgcgtc tcgccaagcg gcgttccgtg
       61 ctctttggct aagaaacact ctcgtcaaac ccaaagggat cggggtcatt cgcgtagatt
      121 cgatcgagga tacgccgctc gcgctcacgc actgtgtcca ggaagtgcct ggcgccgcgc
      181 atgtgcttga agacccgata gccgttctcc aggaaatcgg tcagctcacc ccacccggcc
      241 ttgcgcgcag gccccttgct caccgccagc atcgcgcggg tgagcggtga gcgcacgaca
      301 ccgtcgaggc gagacccaat ttgatgcacc aagtcgatct gccgcacacg ctcgtcgtag
      361 ttgtcgcaaa tgcggtaagc ctcggcgtac agcgccgtgg tgatggtgtc cgtcacaccc
      421 aactgctggg tcagcacgtc aatgaggcgc tggtcgagcg cctcggtgag atgatgtaat
      481 tcgacggtca gcgccagggg acgaatcaac gcgtcgggca ggatgtgctg cagcgcatcg
      541 tgcacggtct ccatgtctcg atcccgctgg ctgaagtcgc gcggcgcgta gacctcgttc
      601 aggaagaaga ggtacgccgg ctggtaacgc ggattggcaa ccagatcggc atatgtgcgt
      661 gccagccgtt cggactgcca cgtacgcagc aaggcgatct tcggatccag cggttgaccc
      721 tcgacggggt gacgcttcag ttgttggaag tcgtggagca tattccctca catgccggac
      781 agcgcgaccc acgccgcgac aaaaacgatg tgcaggactt gatcggccca aatcgccacg
      841 tgccgcgcgt acggcccgtc tccggtctgg tggtacacgc gctgccacca gctcagcggc
      901 agtcgcgtat cgatgagcat gtgaacgata cccagcgcca atccagccgg ccatcccaga
      961 gcgaggccga gcaggagcgc atgaatcgcg ccgtgcaccc acgctgcagg atgccgtaga
     1021 ctgatcttgt tccgggccat ccagtcgttt tgcagcagcc agtcggcaaa gagatgcgcg
     1081 acgaaacctc gctcgaacgg tgtcagcatg cggttccgat cctggcgatc acgtcaatca
     1141 ctcaacacat actcgatagc ctggtccaac gacatcgctc tgccctgggc gtggtaagtt
     1201 tcttgctcgt catcgctcat tgcccgccgg gccggttcca gcatctgctc cagcttttcc
     1261 tgctcgacgg acgagtgtgg cgcgccgatc atgtcacgca gcgcggaggc tgcgccggcc
     1321 agccgaaggg cgcgctccgg ttggctttgc agcgcggcga gcacactcat gtcttcgagc
     1381 aagtacgcca gcgaccagcg atctcccagc tcgcgataga gtacaaggct ttcgcgatag
     1441 tgcttgcgcg ctgtcgtgta atcgcctcgg gagcggacga cgttgcccag gttgttaagc
     1501 gcgacgcccg tgttctgctt atcgccgacc tcgcggttga gggcgatcgc ttcctccagc
     1561 cgtgcgcgcg cttccgtgta gtcgcccagc tccagcgcaa cgaaacccag gttattgagc
     1621 gagacgccga tggcgcggcg atccttcagc tcgcggcgaa tcgccagcgc ttcttcatgc
     1681 aaggcgtggg cgctcgcgta ctcgccgcgc atgcgcgcga cgatgcccag gttgctgagc
     1741 agactggcga tttgcggctt gtcttcgagt ttgcgccgga tcgccagact ctccccatag
     1801 agcgcgcgcg ccgcatccgt gttgccctgc tgggcggcga gggatccggc ataatgaagc
     1861 gtctggccga caccagcctc gtcgcccagc tcctcgaaac ccgcccgcgc ttgcacgagc
     1921 caccgcgcgg cctcgtcgta tcggccgcgt tttcggagca gttcgccggt cgccgcccgg
     1981 ctgcgcgcct gagcttgccg atcgcccaga tctcgggcca gctccagcgc ctgtcgataa
     2041 gcgtcgaggg actcgtccca ccggcccacg acctccagca cctgccccat cttgagcagc
     2101 accggcacct gctgcgcagc cggcagcagc cgcagcacgc gccggtaata gtcgatcgcc
     2161 gccgcgttcg cataccgttc ttgcgcggct tcgcccgccc ggagaagata ctcgcttttc
     2221 ttggcctcgt tttcgctgcg atcgtaatga aacgccagca gatcgacttg ctgctccgga
     2281 gcttgaggat aggactgctc gatgtactgg gcgatctgct cgtgcagcat ggctcgcgtc
     2341 gcgaacggca ggctttcgta cgcgacttcc tgagtgacga tgtgcttaaa gatgtacgtc
     2401 agctccggct cggcgtcgag cgaggtgagc tccaggcgac tcagcgcgtc gagatcggct
     2461 ttgacgtgct gcggcccacc caacggcgga taggcgcccc acagcacggc cgccttgaac
     2521 aatcgcccga ccacactggc gacccgcagc gtcgtcttct gccgctcggt gacttgatcg
     2581 atgcgcgcca ggatcagact gtgcaggctg gccggcaggt cgagctgttc cagcgcccgg
     2641 ctgtcctgtg gatcgatgcc acgatcttgc aggtagttca ggagttcttc gatatagaag
     2701 ggattgccct cggcgcgagc cacgacgcgg tcgacgacgt cgcgcggcgc ggcgacgtgt
     2761 gagccaaaaa agcgatcgag cttcaggcgg atcagctctt cggcctcctg cggcgtgaag
     2821 ctcgtcaacc gaatctcggt gaagtgaggc agttggctca cgcgcagcgc ctgcaggcgc
     2881 tctacatccg gtgggcgata ggccatgacg atcagcacag gcacgtcggc aatggcgcgg
     2941 ccgacggcct ccaacagatc gtgtgaaagc ggatccagcc agtggcaatc ctccagcacg
     3001 aacagcgtcg gcgtcatgcg cgcgcgtccc cgcacacagt cgaccagcag cgactcgaga
     3061 gaggtcttgc gcagttgggc gtcgaagcct tgtgtgagat cgttgtccgg gatcgtcagg
     3121 ccgagcgccg cccccaggag cggcagtcgc ggcacgagcg cgtcgtcgat caggcgcaat
     3181 tgtcgggtca gcaccttgac ttgcttttcc aggctccacg ccggatcgac gccgaagaag
     3241 ctgcgccaga tcgagtgcca caccaggtaa ctcgtgttcg tgccgtagga gagacattcg
     3301 ccgccatacc cgacgatctg ttcctccccc gtgccgcgaa tgatctcggc cacgaggcgc
     3361 gacttgccga tgcccgcctc gccagtgata cccaggattt gtccacgtcc gtcgcgtgcc
     3421 tggtcgatct tctgactggc gatcttcagc tcggattcgc gcccgaccat cggcagcgca
     3481 tagtccggct cctgcaagcg caccgcgcgc cgctctcggg aaccgatcag gctgaaaatc
     3541 gccacaggct cgaccttgcc tttgaccctg atgtcgggca ggcgttccca ctcgaattcc
     3601 gcctcagcgc cctgataggc ggtccggctc gcgagtattt gcccgggcgc ggcggcctgc
     3661 atcaagcggg ctgccaggtt gacgtcatcg cccagcacac cgtacgtacg gcgcatcgat
     3721 ccaccgtacg cgcctgtccg catgcgaccc cggctgatcc cgattcgtgc atcgtggatg
     3781 taagggaagt ccttcttgag gacgtgcagc tcgagcgcgg ccgaagccgc ccgcacgacg
     3841 tcgtcctcgt gcgccagagg agcgccgaag gagatataga ggtagctgcc cttatcgccg
     3901 atcgtcagtt gcagaaggct gccttcgtag cgcgccacga tgccctgaac ggcgcggata
     3961 taggcgtcga gcttggtctg cgcttcctcg tccgcgtcgt agtcgatgcc cccgaacaga
     4021 aggaagagcg ccacggcggg gcggagctcg gccagaaact ctccctggcc gctgctcagc
     4081 cgctcgtaca ccgccggcag cagccagggg cggacttgct cgtcgcgcag ggcatcggcg
     4141 cgaatcggcg cccacgccag cggcggaacg ggcttgacga tgccgctcac tactccgaaa
     4201 cgctggccgt tctcgccgac acgccactcg ccaatgtgca cctgatcggc cagcgcggcg
     4261 acggccgccg ggtcgagcac gacctcgccc ttcccggcct gatgctcggc gacggccaac
     4321 tgatcgagga ttgcgccggc cagcgcgtcg atgagctgga tgcttggatc gccaaccagg
     4381 aagcgccgcg ccgggccggt cgacaccgcc actttcatgg ccagcgccag ggtctggccg
     4441 gaggggagcg ccacgctcgt gaaccgcttc atcgtctgct gcatcgacag cgcgcaggcc
     4501 gtggcgaaca atcccgaatc gccatcaaac cagcatgtga tcgcgtcgcc gctgaagctg
     4561 atcacgcttc caccgtagcg attcagctcg ccgatgatcg cgtcgtagac caggttcaaa
     4621 tggcgcgtca gctcctccgc gccgcgctgc ggccccagct cgcgcgccag cgcttccgtc
     4681 agcggagtga aacccgaaat gtcggcgaac agcgcggcgc cgcgcgtgcg atcgggcagt
     4741 gtctcgccgt cggccagcgc gtgcagtcgg tcggcgggga tgtaggcgat caatgtttcc
     4801 atcgaggttc ccggctacgc cggctcgttc acgacgaggc gcacgatgtc tcggagcata
     4861 gcgctgatcg gatgatcggg gaagcgcagc acgaagactc ccgagctggc cagggccatc
     4921 aggtcgtcgg agtgcggcag cacggcgatg acaggacagt tgtacgcctt ctccacgcgc
     4981 gctttcactt cggcggggtc gaacgcctgc ggcatcttgt tggcgacgag cgccatgcgc
     5041 ggcacgtcga gcttgcgcac cacctcgacc gtaacgcccg tgccctggta gtcctgctgg
     5101 tcgggacgca gaacgatgac gagcgcgtct caaaacccgt agaacgggca tccctatgcc
     5161 cgctgttgac gcatgggaat gcgtcagcca cgagttctaa aacagtctct aagcccgttt
     5221 tccgcaccct ggcccaattc taacagtgct cgcgtggatt gccaacccac gccccgatca
     5281 ctggatttgg cacgcccttc aggcagcagt gggagcgggt gcgaaatctg ggctaagcgc
     5341 cggccaatcg gctcaaccgg attgttgcca gcaaccggca tacccggcgt ccagctcgtt
     5401 cactgagcaa ccgtcgagtc gtatagaggc ccctggtcaa cgtcgccgat ggtaccgctc
     5461 tggtcgtgcg gcatgatccc gactctcacc tgtccatcgc acgagccgcg cccatcgcta
     5521 gcggtgaaga agatgtgata cacgcggccg tcgccgttgc cgtcgcgctc ggcgcgcacg
     5581 tacgctgtgc tcgttccaag acccatcccg tccgccgtgg tgcctgtcgg ctcgtcttgc
     5641 cggatgcccg tgatggtgat cgtgaccatg tcgccgtcgg gatcggtcac gccaagcacg
     5701 gttaccggat ggaagacctt gtccggcggc cagagcgtca cggtgctggc cacggcgtcc
     5761 gtgcacaccg gcacgccgtt gacggaatgg acggtgatca ccactgtcgc gacgttgcta
     5821 tcggccgcgc cgtcgttggc tttgtaggtg aagctgtcgc cgccgtggaa gttggcgtcc
     5881 ggcgtgtaca cgaacgaacc attcgcattc agcgtgagcg cgccgtgggc cgggccgttc
     5941 accaggaccg ccgtcaggcc gtcaccgtct atgtcgctgt cgttgccgag cacgcccggc
     6001 gcgggcacat tcaacgacgt gtcttcatta acgctgtagg cgtcgttccc cgcaaccggg
     6061 ttgtcgttca ccggatggac ggtgatcgac accgtcgcga cgctgctgtc gtcgaagccg
     6121 tcattcgcct tgtaggtgaa gctgtcgccg ccgttgaagt tggcgttcgg tgtgtaggtg
     6181 aacgatccgt ccgcgttcag cgccagcgta ccactcagcg gtccgaacac caaaatcgcg
     6241 ctcaggctgt cgccgtcggg gtcgctatca ttgctcagca cgcctggcgc agcgacgaac
     6301 agggtggcgt cctcattggc gctataggcg tcattcccgg ccaccggagc gcggttgaag
     6361 atggtcagcg agccggtcgc cgtagacgaa atgtagcggt cgttgacgag cgcgagtgtc
     6421 ccgtccggca gcggaatgac gccgctgaag tatgcgttga cggcgtacac acccaccggc
     6481 gccggcacgc cgccgagagg agcacggcct aagtaatcgg tgatgacggc caccgtgtga
     6541 ctgccaccgc tgcccgtcac gacgaagaat accgtcttct ccggcaggcg ccgccccccg
     6601 gcgtctgtca gcgtcgcggt gatcggcgtc cccgcgccgc gcggcacggt ggcgctcgtc
     6661 gggtcgagcg tcagaatcgt atcctgatgc aagatttcga acggggctgt cgcagagctg
     6721 gccaggtaga tcgaagtccc ggcgaaggag gcgcgtactt cgtactggcc cggcagcccc
     6781 agcagcgaga gcgtggccgt ggcaacgccc gtgttgtctg tgaacgccag ccggctctgc
     6841 gggccgaggc ggaagacgac gcgctggccg cccagcggca tcccgtcgct cgtcagcttc
     6901 gcactaaagg tggcgcgtgc cccgtacgtg ccggaactcg gcgcagactg gaattcgagt
     6961 accgccggtt ctgtcggctc cgtaacgttg ccagggatgt agtacgctcc cttgttcgtg
     7021 tccaggccga cgagacccac gccgttcacc gcctgcacga tgtagcgaat gtcccctgcc
     7081 ggcgtgcctg ccagcggcaa ggtcgccgtc cagagcgtcg agtcggtcag gctctgtgcc
     7141 aggtcgagcg actgccatct cccagcgtag ggaccgctta acgcagtgaa ggtgatccac
     7201 gcctcctgca ttccagcgcc ggggctgccc actacattga catggaacgt gacgtttccg
     7261 tcgcccggaa tacctaagac gcgcacgata gagggcgcag ccgcaagggc cggcacgttg
     7321 ccattcgagt acgatgccgt gtggctgctg tagtacaacc ggaagctcat gtcgtcgaac
     7381 tggcgcagcg tgacggtcga agcgccgggc gcgcccgatt gcacctgggc cgggatcacg
     7441 tcgaggcgcg tcacgccggc gccgccggac agcgcatcga agtagttcac gttccagggc
     7501 cggatcggat agaacacatc agagaggaac ggcgcgtggg cggcgcgcag ctcggtcgtc
     7561 gccgcactga ggaacggcag tatgtccggt aagtctgtga agctgccgcc ccggaagccg
     7621 acgccgcgca gcgccaggcc gggcacgctc acgtcccgcg attcgagcgg gagcaccggc
     7681 tcgccggggt tggacaccgt gccgcttgag ccggacagat actgcgccgt gaccgtctgc
     7741 gcgaggttcg ccgtgttcga cagcggcacc gtgtttggcg tcagtgacat ggggatattg
     7801 acgtctgcaa agcgcaggcc gagcgtctgg ccggggtcgg cagcgaagcc gctcgttccg
     7861 ggcacgatcg agccatcggt aggagcgtcg ccgcgtcctg agggcaagtc taccttcagc
     7921 atcggcaggc cgaagagcgt cgcctccagc agagacttct cgtggatgcc gcgcatcact
     7981 ggcgtgttcg ccaggtacgc ctgcttggcc tgcacgagcg cctggccgat agcgaccggg
     8041 tcgctgcccg ctcgcagttg tttggcgaac tcgacgtaca ggcgctcgct gtactcgacg
     8101 aaatcggtgt cgccgtactg gtagccggtc ccggcgacga gcgacgcgcc cttgccattg
     8161 aatgcctgcg cccagtccgg gtcagccgtc acgccgggaa tgccatgcgg gttgacgatg
     8221 ttgtacccgg cgtggcagcc tgcgctgaag accaggacgt tccgccaatc gagcgacgac
     8281 gacacaatct cgtccgcggt cacgcgggtg cgatagtcgg cggcgagcgc gccgccggcg
     8341 ctgaagtgac cggccaggaa gatcaggtca tgacggctgt ccaaaagctg atcgcgcaag
     8401 tcgtccgccg tccaggcgtc cgcgtcttgt ggcgaccgat tgcgcggatc gatgagtgtg
     8461 tcgtgcgacg ccccggctcc catcccggcc accaattcgg cgttgaccgc gttcgccgcg
     8521 tctgcgagga agtcgtagcc ggtcacgagc gaggacgccg gcgtgggcgc gacgccatcc
     8581 gtcgtgctca tatacgcatc gagcatctga atcacgtctg cctcggtttc cacaagccgc
     8641 ccgacggcga ggtccgggat cgggaaactg ttgtttttaa gcgagaggcc gaaccgcgag
     8701 ccgtacgcat cctggctgag cacgaagccg agtctcaggc tggcctgcga ggcggtgttg
     8761 tcgcgcaccg gcggcacgta gttattctcg tttgccaggg aggcattgtc gggataacgg
     8821 aagaacggga tcacattgtc gttgccgacg atgaccgcat attctaaggt agggttggcg
     8881 tcccggtagg catccacaat gcctttgatt gactccgcga cgaggttctt ggcaaatgga
     8941 cactgcgggc gctcgtctgc ctggtcgttg gcgtccgtca cgcggtcgtc taagctcacg
     9001 tcgagtacga cgcccctcac ttcgggccgc gccgcgaagg cgtcgagtgc atccatcagc
     9061 gcgccagtga tgcccatccg gcctgagtcg atcaagatag ccgtcttgta gggaccgtca
     9121 gcggccgaga ccgcagcggc tgagttcgca aggttcgagg gcgggaatcc ggcggagcta
     9181 acgttgctgc acgccccgag ctgctgcacg acttgcaggc ggaacggcga cgccaagctg
     9241 aacgcgccat tgcgcccgcg cacgcgcacg tagaagtcgc ccgtgttgtt ccaggtgttg
     9301 acactgacac cttcgccggc cgtgccatca aacccggaga cggcgatgac gctgcgcgtc
     9361 tgcgcgcccg aaaatatctc cgggctgaag atttctgggc tgaagatctc gggactgaaa
     9421 atctccgggc tgaagatttc cgggctgaag atctcgggac tgaagatctc gggtgacagg
     9481 gcgtccgggc tgaagatttc cggactgaag atctcggggc tgaagatctc cggactgaag
     9541 atttccgggc tgaagatctc ggggctgaag atctccgggc tgaagatttc cgggctgaag
     9601 atctcaggac taaagatctc agggctgaag atctcaggac taaagatctc gggactgaag
     9661 gcgtcgatgg cgaattccgc gcccagatgg gagaggtcct gctgcgcgtt gatctccagc
     9721 agcgaccgga gtgtggcgct gatgtccttg tacaccgtga gatcgtaatt ggccgggagg
     9781 tcggtgagcg ccacgatgac cttgctgccc ggctgcacgc tgaatttgta ccagcgcgac
     9841 tggccttgca gatcgatgta gtgactggcc gaagccgaca ccgtggatcc ggttccggtg
     9901 agcgtcatgc tgaatgcccg cgtccaggcg tcgttcccct gaccaaccgt cacgcaggac
     9961 gaggagcctg acacgttgcc gttcggatca gtcgccgttg ccgtgacaaa ctggccctcg
    10021 gtcgccaggg catcgatcac gctgcggaag tagacattgc cgctatcgtt cgtcgtcacc
    10081 gtggacgaac cgagcagcgt cttcgggccg tcgcaggtag ggctagtgta gaaggtgagc
    10141 gtgaacgtgg tgtttgcggc gctgaagagc cggccatcga ccgtcgtgcc ggcgttgccg
    10201 aggacgacac gaatcacgac gggggagttc tgcgcgcccg cctcgaatgc gccgatgtcg
    10261 caggctctcc cacgcggtcg ataggcgccg cgctgatcgg tcagggagca ggcgtccccg
    10321 ccgctgccgg gcgtggccgg gttgcccgcg tcgatcgccg ggctgccggc cagcagtgcg
    10381 tgcgtgaagg tggcgccccc gttatcttgc agcgagccga gattgaggtc cgggtcagcc
    10441 acgcctaccc ggtcgccatt ggctccgtca acaaagccgg cgcaacctgt gctcggcccg
    10501 atcaagttgt gcccctgcga gatcaggctc cccgagcagt tgggatgttg ggttgcgcca
    10561 ctgttgtccg tgtttccagc gatgatggtg ttcttaacgc cgactgtgcc gccgcttcgg
    10621 aacacaccac cgccgttacc cgatccatta ccgtcactgt cggctgtatt ggcggtaacg
    10681 gtgacgttgt tcagagtcgc agcgctggcg ctgcccgtgt tgtaaaggcc gccgccgtca
    10741 gtcttggccg tgttgccgct gattgtgacg ttggcaagcg tggctgctcc tgcgttgtag
    10801 atgccgccgc ctctgcctcc agccttgttg tccgacacgg tgccgttcaa cagcgtgagc
    10861 gtaccgagat tgtagatgcc gccaccgtca ctgctcacgt tgccactact gatcgtcacc
    10921 ccggaaatag tggcgctccc gcccaggata tggaaaatcc ggtccgaaaa cccactgctg
    10981 gccgtgatcg tcacagggcc ggtcggcgaa atgacgacgc tggcggtgat gtccagatcg
    11041 ccggtgttgc tcgaatcttc tttgccgttg tcagagcggg tgagcgtgta agtgccggcc
    11101 ggcaggacaa tggtgtccac gctgctgcct gcggcgcatt cgccgggatt gttgctggag
    11161 gcctggtcct tattggccgc gatgatggct tcgcggagcg tgcatgcgcc gtccgtgttg
    11221 acggcgtccg ccgtgctgtt caccgcgatt tgaatgtggt ttgatgcgc
//
LOCUS       DBNHXB010000276         9143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3259747, whole genome shotgun sequence.
ACCESSION   DBNHXB010000276 DBNHXB010000000
VERSION     DBNHXB010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9143
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3259747"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="AC162G_11130"
     CDS             complement(<1..797)
                     /locus_tag="AC162G_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5076416.1"
                     /translation="MKPTRATSVQASDNDTQSAASLRMTQRQAIRDRQAAVLRRTIGN
                     ALLYVVLCLVAVITIFPFAWIFFTSLKGPTDPIYSVPPQLIPQAPTLDNYVRVWNLLP
                     VGNFYLNSIIATVSVVILDTLVCSLAAYPLAKMRFRGRGVIFYALLATLIVPPVLTSI
                     PSFILAIKVFQYHDKLTSVIFPLLANVFNIFLLRQAFKGVPDDLMDAARIDGASELRI
                     WRSIMMPIIRPSLATVAIIIFVTQWNNFFWPSLMLNTMENKTLPVGLV"
     gene            complement(794..1672)
                     /locus_tag="AC162G_11135"
     CDS             complement(794..1672)
                     /locus_tag="AC162G_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5076417.1"
                     /translation="MTRKFSLTPYLFLAPALLVIAIFVVYPIVAVVYYSFTEYNISTP
                     PVWTDLKNYQRLMRDPVFWQALKNSFIYLLVTPTLIFLCILLAIVVNRRLPGISAFRA
                     MFFIPVISGTIAVGIAWRLMWERNGIINGLLMSLGLLQEPIQWLAEPAYTLPIAMLLT
                     TWAGLGYYMMIFLAGLQNIPDELYDAALIDGCNAWQKHWHVSIPGLRPQITFVAVISS
                     LAALGVFNEILTLTGGTGGILNSGVTMVFYLWRQTFRLSNAGLGSAIAVVFLVISLAF
                     SIANMRIVERGSESQG"
     gene            complement(1742..3121)
                     /locus_tag="AC162G_11140"
     CDS             complement(1742..3121)
                     /locus_tag="AC162G_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5076418.1"
                     /translation="MRTKFLYTLTLVSLLLAACGPAATATSEIIRETVVVTQPAEVVE
                     KIVEVTPAPGPHPEAVIEGVESNAEITVWTFWLSPTFDDYIKSTIARFEEAYPGVTVN
                     WEDHQATFQDDLRNSFAAGNAPDVINLSISEGWVTDYATQGLLLPLDDVVPDDVKDLY
                     FPNLWDQQVVDDQHYMFPYYQGIIVNLVNTKIISDAGLTVEDFPKTVQELPAFCQIIK
                     DETSTLCDIRLTVNDLLAQMTYEGGVQVINEDGTEFTFDSPEAVEWLQMYVDMVAAGT
                     VDRAVLVTDQDRVGLDLFTTGQAAFYNTGPNLIREVRANNPGLYGYLAVVPAPVGKSG
                     VVGKGLMAMSVKADTEFPNASIALAQFMTNPRSMLEFSKIVAIYPSTPASYDDPFFSA
                     APVAIEDSAKPYAKDAISKLADIVPSVPNKAEVNNIVLQAVQQALFNNVDPQQALSDA
                     VEQANALLP"
     gene            complement(3135..4166)
                     /locus_tag="AC162G_11145"
     CDS             complement(3135..4166)
                     /locus_tag="AC162G_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR5076419.1"
                     /translation="MGGTKVAVLVVDAWRHIRSRVVVPPDLTSPQRTLTGIAAAIRQA
                     LEMGSASLHEVAGIGLGIPGRVDPQTGVVHLAVNLNWRDMSVRAWLSEELGVPCFLEK
                     DVRAAALGARLCKGNSSVQNMAYVSVGTGIGAGVILQGRLYHGTHGMAGEIGHMIVEP
                     NGPRCQCGAQGCLEALAAGPAIARLAQQAVSSGQETRLRDYSPLTAESVYQAAEAGDA
                     AAQAVADVVGHRLGRALQVLVMTYDVEEVVLGGGVSRAGHAFLEPILRELERQRQQSA
                     LAREMLRLEMFKLLPSNCDAGTWGAVAIAEQSLRDSFREKGGVNEISHTLPALEEPFD
                     NRESNLSQS"
     gene            complement(4244..5452)
                     /locus_tag="AC162G_11150"
     CDS             complement(4244..5452)
                     /locus_tag="AC162G_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HIR5076420.1"
                     /translation="MKTHPLKATHKQTKAYNSQLVLKTIYDGGQISRADVARATGLTR
                     TSVSELVPHLLRQGLVEEVGRGPSAGGKSPILLKVADDARHLIGIDLASSVFRGAVVS
                     LRGEIRHTVNLPIQTRRGEDALELVYELIDSLAASTDKPLLGIGIGTPGLIDTANGVV
                     QQAVNLDWRDLPLGSLLRARYNLPIYVANDSQLAALAEHTFGGGQGASNLVVLKVGRG
                     IGAGIVLNGQLFQGDGSGAGEIGHIAIVEDGLQCRCGNFGCLETVASTRAIVQRAQAL
                     APSHPHSLLSRFSNQPDAITTDTIQAAFQAGDDLARQIVLEAGRYLGMAVAGLVGALN
                     VQCILVVGNMARFGQPWLEAIRQEMLKRSLPMLAQRTQIEIGQLEPNVVILGASAMLL
                     TRELGLSLAR"
     gene            5911..6654
                     /locus_tag="AC162G_11155"
     CDS             5911..6654
                     /locus_tag="AC162G_11155"
                     /inference="COORDINATES: protein motif:HMM:NF019844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR5076421.1"
                     /translation="MKLIIRLVLTALVLALSVAATAGSASAAPLEGSAILGLHTVRTG
                     ETLYCIGRAYSVQPWAIAAQNGIGQASKLYVGQVLAIPDAPWINRPAGPTCARQFDPG
                     TPPPPPPDCQVTYTVTYGDTLLRIARRFNVNVYTLAARNGIYDLNRIFVGQVLCIPGS
                     GKPAPDIVIVLPTDVQYVLALSNVNMRTGPGLEFAVIGKVYAGQIAGVTGVSGNGQWW
                     RVICPDNTVGNCWVSADPSLTQPTTPPGW"
     gene            6677..7444
                     /locus_tag="AC162G_11160"
     CDS             6677..7444
                     /locus_tag="AC162G_11160"
                     /inference="COORDINATES: protein motif:HMM:NF016300.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR5076422.1"
                     /translation="MKSNRWYFVLGAVAVTALAIVAGAVISLIPTATTVAVEPVVAYT
                     PAQASDAARSITVVGTGSVSIAPDRATATIGVDTQAASPDEATGQNDERVATVIDALK
                     AAGIEPADIQTAYYNLYAEQRFDPGTGQPTGEFTYRVSTGLSVTVRDLPRLGSLLNDA
                     VKAGANNISGVSLSVADQAAFEASAREKAIADAKARAQALAELSGVTLGEVVSVSEVI
                     GGVAPVFDRGLGGGGAPIQPGAIEVRMQVQVSFAIGQ"
     gene            7654..8247
                     /locus_tag="AC162G_11165"
     CDS             7654..8247
                     /locus_tag="AC162G_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076423.1"
                     /translation="MLAVSACAPAGTETPDSNARPILADGWQAFKADSGYEIRYPLEQ
                     YSIRNAPSSPEVLYPGVKVIEPNDAFYYAEPRVVTYKISIAVSENTQNLSLDDPAQLL
                     AHSVIYPYDPGALAGHNIREVTLDGVKALRVDNLEVGPAGVTAQIVTIRSNRIYELLV
                     EPHQVVGNQAVPFQMGEASPENQELFEAIIATFRFSE"
     gene            8268..>9143
                     /locus_tag="AC162G_11170"
     CDS             8268..>9143
                     /locus_tag="AC162G_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5076424.1"
                     /translation="MKTKRLTTMGVIAAFVLAACAPIQTGSTVSITAAPTVASTHTPG
                     LTPSASATQAQAPTPQSSTALYRGFVVYDEGTAQFTAYDFQGKPLNFTVSARHQQVWR
                     APDETQVVGDAIYYRDEDQTIVRADADGTRKLSGIPAENLSVFKVSPDETMIAWTSNL
                     WDQTPPQSELWIANLDGSNARKVATSTTDASSELFVPTPYRWTDDGKLLYVETPTGIG
                     GYILYRAYSSLHVFDRGTGEINPLYQPEDKYSVCLSGVASDLSRVGFGCGEGGAGRVS
                     ILTLADGKTTPVPDLA"
BASE COUNT         1884 a         2736 c         2808 g         1715 t
ORIGIN      
        1 accagcccaa cgggtaaggt cttgttctcc atcgtgttca gcatgagcga tggccagaag
       61 aagttattcc actgggtaac gaagatgatg atggcgacgg tggccagcga aggacggata
      121 atgggcatca tgatactccg ccagatgcgc agctcgctcg caccatcaat gcgtgccgcg
      181 tccatcaaat cgtcaggcac gcccttgaac gcctgccgca gcagaaagat gttgaacaca
      241 ttcgccagaa gcgggaagat cacggaagtc agcttgtcgt gatattggaa gaccttgatg
      301 gcgaggataa agctagggat tgaggtcagc acgggcggta caatgagcgt cgccaggagc
      361 gcgtagaaaa tcacgccgcg cccccgaaat ctcatcttgg ctaaaggata cgcggcgagt
      421 gaacagacga gcgtgtccag gattacgacg ctcaccgtcg caatgatgct attcaagtag
      481 aagttcccaa ccggcaatag attccagacc ctcacatagt tatccagggt cggagcctgc
      541 ggaatgagct gcggcggcac ggaatagatc ggatcggttg gccctttgag cgaagtgaaa
      601 aatatccaag cgaaagggaa gatggtgatg acggccacaa gacagagcac gacgtacaac
      661 aaagcattcc cgatcgtacg gcggagcacc gcggcctggc ggtcacgtat cgcttgccgt
      721 tgcgtcatgc gcaggctggc ggcgctctgg gtatcgttgt cagaggcttg gacacttgtc
      781 gctcgcgtgg gtttcatcct tgtgactccg aaccgcgctc aacaatacgc atattcgcaa
      841 ttgaaaaggc gagcgagatc accagaaaca cgaccgcgat tgccgagcct agtcccgcat
      901 tactcaacct gaatgtctgc cgccagaggt aaaacaccat cgtcacgccg ctgttcaaaa
      961 tgccacccgt gccgccggtg agcgtcaaaa tctcgttgaa cacacccaga gcggccaggc
     1021 tcgagatgac ggcgacgaag gtgatctgcg gacgcaggcc gggtatgctg acatgccaat
     1081 gcttctgcca ggcattacag ccgtcaatca gcgccgcgtc gtacagctcg tccgggatgt
     1141 tctgcagccc ggcgaggaag atcatcatgt aatagcccag gcccgcccag gtggtcagca
     1201 gcatggcgat cggcaacgtg taagccggct cggccagcca ttgaatgggc tcttggagaa
     1261 gacccagcga catgagaagg ccgttaatga ttccgttgcg ttcccacatc aaacgccagg
     1321 caatgccgac ggcaatggtg ccgctgatga cggggataaa aaacatcgcg cgaaaggcgc
     1381 tgatgccggg cagtctacga ttgacgacga tggccaggag gatgcatagg aagatcagcg
     1441 tcggcgtcac cagcaagtaa atgaatgagt ttttcagcgc ctgccaaaag accgggtcgc
     1501 gcatcaatcg ttgataattc tttaagtcag tccagaccgg cggcgtcgag atgttgtatt
     1561 cggtaaagct atagtagacg acggctacga tggggtatac gacaaagatc gcaatcacga
     1621 gcagagcggg cgcgaggaaa aggtagggcg ttaaagagaa ctttctggtc atgcgggggt
     1681 ctccgcaggg ccggccctct cccggaaacg ggagagggcc gggggcgtag cgggtgtttg
     1741 cttacggaag caaggcgttt gcctgctcga ccgcgtccga caatgcctgc tgaggatcga
     1801 cattgttgaa cagcgcctgt tgaaccgcct ggaggacgat attgttgacc tcggccttat
     1861 ttggaacgct gggcacgatg tcggccaact tggagatagc atccttggcg tagggcttgg
     1921 cgctatcctc gatggcgacc ggcgcggctg agaagaacgg atcgtcatag gacgccggcg
     1981 ttgaggggta gatggctacg atcttggaga actcgagcat gctgcgtggg ttggtcatga
     2041 attgcgccag agcaatcgac gcattcggga actcggtgtc cgctttcacc gacatggcca
     2101 tcagaccctt gccgacgacc cccgacttgc ccaccggcgc aggcacgacg gcgagataac
     2161 cgtacagccc ggggttgttg gcgcgtacct cgcggatgag attgggaccc gtgttataga
     2221 aggcggcctg gcccgtggtg aagaggtcaa gccccacgcg atcctgatcg gtcaccagca
     2281 ccgcccgatc caccgtgccg gctgcgacca tgtccacgta catctgcagc cattcgacgg
     2341 cttccggcga atcgaaggtg aattccgtgc cgtcttcgtt gatcacctgg acgccgcctt
     2401 cgtacgtcat ttgcgcgagc agatcattca ccgtcaagcg gatgtcacac aaagtgctgg
     2461 tctcgtcctt gatgatctgg caaaacgcag ggagttcctg aacggtcttc ggaaaatcct
     2521 cgaccgtcaa cccggcgtcg ctgatgatct tggtgttgac gaggttcacg atgatcccct
     2581 ggtagtaggg gaacatatag tgctggtcgt caactacctg ctggtcccac aagttgggga
     2641 agtacaggtc cttcacgtca tcgggcacaa catcgtccag cgggagcagt aagccctgcg
     2701 tcgcatagtc agtgacccag ccttcggaga tggacaggtt gatgacgtcg ggcgcgttcc
     2761 cggcggcgaa cgaattgcgt agatcatcct ggaaagtggc ttgatgatct tcccagttga
     2821 ccgtgacgcc ggggtacgct tcctcgaagc gggcgatggt gctcttgatg taatcgtcaa
     2881 acgtcggtga gagccagaaa gtccaaaccg tgatctcggc gttcgactcg acgccctcga
     2941 tcaccgcttc ggggtgcggg cctggcgcgg gcgtgacctc tacgatcttc tccacgacct
     3001 ccgcgggctg tgtgacgacc actgtctctc gtatgatctc ggaggttgcg gtcgccgccg
     3061 ggccacacgc ggcgagcagc aggctgacca gcgtcagcgt atacagaaac ttcgtgcgca
     3121 tgtgttatct cctcctagga ttgggatagg ttggattcgc ggttgtcgaa tggctcttca
     3181 agtgcgggca gagtgtggct gatttcgttc acccctcctt tctccctgaa cgagtctcgc
     3241 aatgactgct cggcgatggc gaccgctccc caggtccccg cgtcgcagtt cgacggaaga
     3301 agcttgaaca tttcgaggcg cagcatctcg cgggccagtg cggactgttg gcgctggcgc
     3361 tcaagctcac gtaagatcgg ctccaagaac gcatggcctg cgcgcgacac gccgccgccc
     3421 agcacgacct cctctacatc gtaagtcatc accaatacct gcagtgctcg ccctaaccgg
     3481 tgtcctacga catcggcgac ggcttgtgcc gccgcgtcgc cggcttcggc ggcctggtaa
     3541 acgctttctg cggtcaatgg cgagtagtcg cgcaggcgtg tctcctggcc ggaggagaca
     3601 gcctgctgtg ccaaacgcgc gatggccgga ccggcggcca gtgcttccag acaaccctgc
     3661 gcgccgcact ggcagcgcgg gccgttcggc tccacgatca tgtgcccgat ctcgcctgcc
     3721 atgccgtgcg ttccgtggta gagccggccc tgcaaaatca cgcctgcgcc gatgccggtg
     3781 ccaacgctga cgtaggccat gttctgaacg gatgagttgc ccttgcaaag ccgcgcaccg
     3841 agcgccgccg cccgtacatc cttctccaag aagcagggca cgcccagttc ttctgagagc
     3901 cacgctctaa cggacatgtc gcgccagttc agattgacgg ccagatgaac cacacccgtc
     3961 tgcggatcga cgcgccccgg aatgccgagg ccgattccgg cgacctcgtg aagcgaggcg
     4021 ctgcccatct ccagggcctg ccggatggcc gccgcaatgc ccgtcagtgt gcgctgcggc
     4081 gaagtcaggt ccggcggcac gacgacccgg cttcgaatgt ggcgccacgc atcgacgacg
     4141 agaacggcca ctttcgtgcc gcccacgtcc acgccaacaa gcaggtcccg tgaagagtcg
     4201 cgtgaatcga tcaacgctgt gcgtgttcgt gtgtgtggtt gatttaacga gccaagctga
     4261 ggcccagctc acgcgtcaac agcatggccg aggctcccag aatcacgacg ttcggttcaa
     4321 gctgcccaat ctcaatctgc gtgcgctgcg ctaacatcgg cagtgaccgc ttgagcattt
     4381 cctgccggat cgcctccaac cacggctgac caaagcgggc catgttgccg accaccagaa
     4441 tgcactggac gtttagcgca cccaccagac cggcgacagc catgcccaaa taacgtcccg
     4501 cttccagcac gatctggcgc gccagatcgt cgccagcctg aaacgctgcc tggatggtat
     4561 cagtcgtgat cgcgtcaggc tggttcgaga aacgactcaa aagagagtga gggtgactcg
     4621 gggcgagagc ttgcgctcgc tggacaatgg cccgcgtgct ggcgaccgtc tccagacagc
     4681 cgaagtttcc acagcggcac tgcagcccgt cttcgacgat ggcgatgtgg ccgatttcac
     4741 ccgcgcctga tccatcaccc tggaacaact gcccgttgag cacgatgccg gcgccaatgc
     4801 cgcgccccac cttgagcacg accagattcg acgcaccttg tccgccgccg aacgtgtgct
     4861 cagccagggc ggccagttgg ctgtcgttcg ccacataaat cgggaggttg taacgcgccc
     4921 tcagcagact gcctagcgga aggtctcgcc agtccagatt gacggcttgc tgcaccacgc
     4981 cgttcgcggt gtcgatcagg cccggcgtgc cgatcccgat gccgagcagt ggcttgtccg
     5041 ttgaagccgc cagcgagtcg atcaactcgt aaacgagctc cagcgcatcc tcaccgcgac
     5101 gagtctgaat cggcagattg acggtgtgcc gaatctcacc gcgcaggctg accacagccc
     5161 cgcggaatac actgctcgcc aggtcaatgc cgatcaagtg ccgtgcgtca tcggccacct
     5221 tcaggaggat aggggacttg ccgccagcag atggaccgcg cccgacttcc tcgaccagcc
     5281 cctgcctgag caggtgggga accaactcgg acacgctcgt gcgcgtcagt ccggtggcgc
     5341 gagccacatc ggctcggcta atctgcccgc cgtcgtagat ggtcttcagc accaactggc
     5401 tgttataggc ttttgtctgc ttgtgagtgg ccttcagggg gtgcgttttc acagcgacga
     5461 tttcggcgta agactttgaa agtaaactga acttacaatg tattgtatca cgaattcgta
     5521 gtttgtcaag agagtttaca aactgtttca tatttcacaa acccgcctgc tgctgcaagg
     5581 agatacgggc ggcctgcccg ctctgctgga agagggactg ttcggcagcg cgcaagaggc
     5641 attggacaat ttcatcccgc atgctccgca ggtcgaagcg gtgacggcca agcacaaata
     5701 catcgtttgg ccgacttcgc cgttccggcg aaggtcagga acgctttccg acatttgcat
     5761 cacgacaggc gctgtgcacc ggtgtatatc tatcgcatca gccgccattc tgcgagcggc
     5821 gttgctggaa tcgaggaaac gacaaacgtc gagttgattc ggacctggct gatgcgactg
     5881 cagccacatt gagcatagag gaggtcaatc atgaaactca tcatccgtct ggttctcacc
     5941 gctttggtgc tcgctttgag cgtcgccgcg acggcaggtt cggcctccgc cgcgccactg
     6001 gaaggatcgg cgatcctggg cctgcacacg gtgcgcactg gggagacgct ctactgtatc
     6061 gggcgcgcgt acagtgtgca gccgtgggcg attgccgcac agaacggcat cgggcaggcg
     6121 agtaagctgt atgtcgggca ggtgctggcg atcccggacg cgccgtggat caaccggccg
     6181 gccggcccga cgtgcgcgcg gcagttcgac cccggcacgc ctccaccgcc gccgcctgac
     6241 tgccaggtga cgtacaccgt cacgtacggt gataccctgc ttcgcatcgc acgccggttc
     6301 aacgtcaatg tctacacact ggcagcgcgg aacggcattt atgaccttaa ccgaatcttc
     6361 gttggacagg tgctgtgcat cccgggttcg ggcaagcctg cgccggacat cgtcatcgtc
     6421 ctgccgacgg atgtgcagta tgtcctggcg ctgagcaacg tgaacatgcg cactggaccc
     6481 ggcctggagt ttgccgtgat aggcaaagtc tacgccggtc agatagccgg ggtcaccggc
     6541 gtgagcggca acggtcaatg gtggcgagtg atctgcccgg ataacaccgt gggcaactgc
     6601 tgggtctccg ccgatccgag cctgacgcag ccgaccacgc cgccgggatg gtaggaacaa
     6661 caaggagaat gacgacatga aaagcaaccg atggtacttt gtgctcggag cggtggcggt
     6721 gacggcgctc gccattgtag ccggcgccgt gatcagcctc atcccaacgg ctacaactgt
     6781 cgccgttgaa ccggttgttg cgtacacgcc tgcccaggcg agcgatgccg cacggtcgat
     6841 caccgtcgtg ggaaccggct cggtgtcgat cgcgcccgat cgggccacgg cgacgatcgg
     6901 cgtggacacg caggcggcct cgcccgacga ggcgactggc cagaatgacg aacgggtggc
     6961 caccgtgatc gacgcgctca aggccgccgg catcgaaccg gcggacatcc agacggccta
     7021 ctacaacctg tacgccgagc agcgcttcga cccgggtacg ggccagccga ccggcgagtt
     7081 cacctaccgc gtcagcaccg gcctgtcggt gacggtgcgc gatctgcccc gcctcggcag
     7141 cctgctgaac gatgcggtca aggccggcgc caacaacata tccggcgtgt cgctcagcgt
     7201 cgccgatcag gccgcgttcg aagccagcgc tcgcgagaaa gccatagctg acgccaaagc
     7261 ccgcgcacag gcgttagcgg aattgagcgg cgtgacgctg ggcgaagtgg tgagcgtgag
     7321 cgaggtgatc ggcggtgttg ctccggtctt tgatcgcggc ctgggcggag gcggcgcgcc
     7381 gatccagcct ggcgctatcg aagtgcggat gcaggtgcag gtctcgttcg cgattgggca
     7441 gtgacggtgt aaacagcgtt ggacggccaa aagcggaccg gaaggcctgc cgcctaacac
     7501 gcgtatgtag tccgaccgcc ttcggcgcgc aagattgtgg ctattttgaa gtgatcttgc
     7561 gtagtgcgcc gaaggcggcg actgatacgc aagccattag gaggataagc catattacga
     7621 caatccaaag ctgccgccat cctgatgttg gcgttgctgg cagtctcggc ctgtgcgcca
     7681 gcgggaaccg agacgccgga ttcaaacgcc cgacccatct tggccgatgg ctggcaggcg
     7741 ttcaaggccg acagcggcta cgagattcgc tatccgttgg agcagtattc gatccgtaac
     7801 gcgccttcat cgccagaagt gctgtaccct ggcgtcaagg tgatcgagcc gaacgatgcg
     7861 ttctattacg cggaaccgcg cgtcgtgacg tacaagatat cgatcgccgt gagcgaaaac
     7921 acgcagaatc tgtcgctgga cgatccggcc cagttgctgg cgcacagcgt catctacccg
     7981 tacgatccgg gcgcgctggc cggccacaac attcgcgagg tcacactgga tggcgtgaag
     8041 gcgctccgtg tggataacct cgaggtcggt ccagcgggtg tcacggctca gatcgtaacg
     8101 atccgcagca accggatcta cgagctgctg gtcgagccac atcaagtcgt cggcaatcag
     8161 gccgtgccat tccagatggg cgaggcttcg ccggagaacc aagagctgtt cgaggcgatc
     8221 atcgccacct tcagattcag cgagtgattt cttcccagga gacccgcatg aagaccaaac
     8281 gactcacaac catgggggtg atcgccgcgt tcgtgctggc cgcgtgcgcg ccaatccaaa
     8341 ccggatcgac ggtgagcatt acagcagccc cgactgtcgc gtctacgcac acgcctggcc
     8401 tgacgccatc tgcatcggcg acgcaggctc aggcgccaac acctcaatca tcgaccgcct
     8461 tgtatcgggg cttcgtcgtc tacgacgagg gcacagctca gttcacggcc tatgattttc
     8521 aaggcaagcc gctgaacttc acggtctctg caaggcacca acaggtgtgg cgcgctcccg
     8581 acgaaacgca agtggtgggc gatgcgatct actatcgcga tgaagaccag accatcgtcc
     8641 gcgcggatgc ggatggcact cgaaaactct ctggcattcc cgcagagaat ctgagtgttt
     8701 tcaaggtgtc acctgacgaa acgatgattg cgtggacgag caatctatgg gatcagacgc
     8761 cgccgcagag tgagttatgg atcgctaacc tggatggctc aaatgcacgc aaggtcgcga
     8821 cttcgaccac ggacgccagc agtgaattgt tcgtgcccac gccttatcgc tggacggacg
     8881 atggcaagct gttgtacgtc gaaacgccga ccggcattgg tggctacatt ctttatcggg
     8941 cgtacagcag tttgcacgtg ttcgatcggg gaacgggcga aatcaatccg ctctatcagc
     9001 cggaagataa gtacagcgtg tgcctgagcg gtgtggcgag cgatctatca cgtgtagggt
     9061 ttggctgcgg cgaaggcggc gcaggcaggg tcagcatcct cacgctggcc gatggcaaga
     9121 ccactcccgt gcccgatctg gcc
//
LOCUS       DBNHXB010000277         5748 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_240287, whole genome shotgun sequence.
ACCESSION   DBNHXB010000277 DBNHXB010000000
VERSION     DBNHXB010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_240287"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2867
                     /locus_tag="AC162G_11175"
     CDS             <1..2867
                     /locus_tag="AC162G_11175"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5076425.1"
                     /translation="RARASSARGQAAREGKQRARAGGYTDARPDGSAAPSLRSGLRLT
                     TSLLDDARNVFSFGALLAAVFAAVGVIGIILAPPGPRLLPATACGLFSFLLSWAGMFL
                     APRSGNPLAAIRLVLIGYVLAVTLAIHFAGGPQSFLTALYVAVTVAAAFLLGRRGALG
                     IALFSAASFSVLIGLEFTGAIPIYPIWEPSLSLEGRGLVLGGLIFSVIVPTFIVANVA
                     GRLADRLSRRTGEQAALAQIARNIASSLDPDEVIRTVLQHAVEDTDSDRGGIYLYDQE
                     RQQMVDAATIGFGRSLQESGGDHSWAGLDGIVGRVLRSGESARVPDVSRDPDYVEAAP
                     TTRAELCVPIIRDGRVEGVINLESDRLAAYSESHPRVVEQLAQHAAIALSNARLYAKT
                     EQNLYEVARANLEVHALQESLSAVQSAPELDAVLQRICDAIVTLGYDLATVATVERAE
                     SRLASRAVAAGEADLIRSLETLLGAELVGMRAQLRRARNIGVRALTERRVLVSRDPID
                     FLYPIGSRMRRSRAFEAVGLRVGAAIPLVVRDESLGVLFAFSRKPDLTDADLSSLQAF
                     GAQAAVALDKAKLLEEARISRDRLQAVLDATRDGLIFFDAQMHMVLTNRAAEALLGIS
                     LSPYVGQPMRRVLEQSGLIERLYPNLNPEERQAALDTEVNTVTAGLRDGSSEVARRLI
                     VVPGVETRYVEEFNLRVDDDEGQFAGRLMVLHDVTHQKQLEADRDAFTQMLVHDLRSP
                     VSAVISGLQLIELAIQEGDPPDLLLRSARASLASANKLLNLIGALLDIQKLETGQLEL
                     QLQPVAPPSLVREVVDILRPLAEMSGVRLEMDAPEDTPPVQGDPDHLRRVLTNLVDNA
                     LKFVGEAGRVRVSVAADGEFVRFSVADNGPGVPLEYRERIFGRYVQVPGRTARRRGTG
                     LGLAYCRMIVEMHGGKIWVESSPLGGSDFIYTLPVAEARE"
     gene            2864..3145
                     /locus_tag="AC162G_11180"
     CDS             2864..3145
                     /locus_tag="AC162G_11180"
                     /inference="COORDINATES: protein motif:HMM:NF013401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR5076426.1"
                     /translation="MTRDGLTSDECSSFFEDMMHAARAVECVFRPLKMNFELLGNAVP
                     HLHAHIVPRYYGDPAPGRPLDPMAQQVTLTLPEYEERIRQLREALLAVG"
     gene            complement(3188..3607)
                     /locus_tag="AC162G_11185"
     CDS             complement(3188..3607)
                     /locus_tag="AC162G_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076427.1"
                     /translation="MWIDHVGYNIIRLSTRGESHECYTDGLNCVSPCGTTLTAEAAVH
                     IAENFIADHLGNLALAGIPRRIIFPIRALWAVPMAVAYPRYGLAGVIGVVAIDDEFAS
                     VVAATPIEEMRQAAEQLYVEHQSDIETAFRQITSAND"
     gene            3593..4285
                     /locus_tag="AC162G_11190"
     CDS             3593..4285
                     /locus_tag="AC162G_11190"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5076428.1"
                     /translation="MIDPHLDNKVILITGANHGIGAATAEAFAAQGAKVFITYYRGAC
                     RHSDEELAQARQAGIGGDVLVNNHTHCVPETFDPALATDQDGGIHLVSAVGIDAHFAI
                     NARAYALMMSEYLARYLKRSAESGRIVNVSTDAAHAHTANVSYAASKHAIESYSRSAA
                     AEMGRYGITINVVAPGPIQTGYVTPEAEAPLRRVGEPEDVADVIVFLASAQARWLTGQ
                     LLYVGGGWRMAQ"
     gene            4353..4997
                     /locus_tag="AC162G_11195"
     CDS             4353..4997
                     /locus_tag="AC162G_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076429.1"
                     /translation="MEQPFWIDEPYPFFPYGASKPGARASILPHALLLGCQLVFLAYI
                     AAELRFPPGGWILFGLLSLIPLTGIYLQLRRKSLTARAALVQQRAREQIGASTMGSAI
                     HVAGHPLLDREQPVVIALRGDQLLFFSYQNRNPIDVLPIGELQDIRTVVYDDDRVPHV
                     DVIDSTAQALQLTFTWRGHTCTSLFRRMHKVRPIDWYQAIQQARLLSVTHVNAA"
     gene            complement(5048..5518)
                     /locus_tag="AC162G_11200"
     CDS             complement(5048..5518)
                     /locus_tag="AC162G_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5076430.1"
                     /translation="MQATHVLETCLYVDDLVAAEDFYTRVCGLKRISRVVGRHVFFRC
                     GDGVFLLFNPEATSQPSDSVSSVPTHGARGPGHVAFAMRHADIDAWREHLRRNDVEIE
                     KEMAWPGEGGLPYLPTWPCQLATNRAAYEFSSARYYDLGKPPIVSVDDVREWLT"
BASE COUNT         1035 a         1760 c         1851 g         1102 t
ORIGIN      
        1 agcgcgcgag ggcaagcagc gcgcgagggc aagcagcgcg cgagggcaag cagcgcgcga
       61 gggcaggcgg atacacggat gcgcgccccg atggttcggc tgccccttca ctgcgttcag
      121 ggcttcggct caccacgagt ctgctcgacg acgcccgaaa tgtcttttcc tttggggcac
      181 tgcttgcggc cgtcttcgcc gcggtcggcg tgatcggtat catcctcgcc ccgcccggcc
      241 cacggcttct gccggcgacg gcatgcggcc tattcagctt cttgttgtcg tgggcgggca
      301 tgttcctcgc gccgcgcagt ggaaaccccc tggcggcgat ccgcttggtc ctcatcggct
      361 acgtgctggc cgtgacgctc gcgattcact tcgccggcgg cccgcagtcc ttcctgacgg
      421 cgttgtacgt agctgtcacg gtggcggcgg cgtttctgtt gggacgacgc ggcgcgctgg
      481 gaatcgcgct gttttcggcg gcgtctttca gcgtcctcat cgggttggag ttcaccggcg
      541 cgattccgat ctatcctatc tgggagccga gcctgtctct ggaagggcgc ggcctcgttt
      601 tgggcgggct gatcttcagc gtgatcgtgc cgactttcat cgtggccaac gtcgccggca
      661 ggctggccga tcgcctctcg cggcgcacgg gcgagcaggc ggcgctggcg cagatcgccc
      721 gcaacatcgc gtccagcctc gaccccgacg aggtcattcg cacggtgctt cagcacgccg
      781 tggaagacac ggacagcgat cgcggaggaa tctacctgta cgatcaggaa cgccagcaga
      841 tggtggatgc ggcgacgatc gggttcggcc gctcgctgca ggagagcggc ggcgaccact
      901 cgtgggcggg gctggacggc atcgtaggcc gggtgctgcg ctccggggaa agcgcgcgcg
      961 tccccgacgt ctcgcgcgat ccggattacg tggaagcggc gccgacgacg cgagccgagc
     1021 tgtgtgtgcc gatcattcgg gatgggcgcg ttgagggcgt gatcaacctg gaaagcgatc
     1081 ggcttgcggc ttacagcgaa tctcacccgc gtgttgtgga gcagctcgcg cagcacgcgg
     1141 cgattgccct gtccaacgcg cgcctgtacg ccaagaccga gcagaatctc tacgaagtcg
     1201 cccgcgccaa cctcgaagtc cacgcgctgc aggagagcct gtccgcggtg cagtcggcgc
     1261 cggagctcga cgcggtgctg cagcgcatct gcgacgcgat cgtgacgctg ggatacgatc
     1321 tggcgacggt ggcgaccgtc gaacgcgccg agtccaggct ggccagccgc gcggttgccg
     1381 ccggagaggc cgatctgatt cgatcactgg agactcttct gggcgctgag ctggtcggga
     1441 tgcgcgctca gttgcgccgg gcgcgcaaca tcggcgtgcg ggcgctgacg gagcggcgcg
     1501 tgctggtgag ccgggatccg atcgactttc tctatccgat cgggtcgcgg atgcggcgca
     1561 gccgcgcgtt cgaggccgtc ggcctgcgcg tgggcgcggc cattccgctg gtggtgcgcg
     1621 acgaatcgct gggggtcttg ttcgccttca gccgcaagcc cgatctgacg gatgcggatc
     1681 tgtcgtcgct gcaggcgttc ggggcgcagg cggcggtggc gctggacaag gccaaactgt
     1741 tggaggaagc gcgcatctcg cgcgaccggc tgcaggcagt gctggacgcg acgcgggatg
     1801 ggctgatctt cttcgacgcc caaatgcaca tggtgctgac caaccgggcg gcggaggctc
     1861 tgttggggat ctcgctgtcg ccgtatgtgg gccagccgat gcggagggtg ctggagcaga
     1921 gcgggctgat cgagcgactg tatcccaatc tcaacccgga agagcggcag gcggcgctcg
     1981 atacggaggt gaacacggtc accgccggct tgcgcgacgg ctcgagcgag gtggcgcgcc
     2041 gcctgatcgt ggtgcccggg gtcgagacgc gttacgtgga ggaattcaac ctgcgtgtgg
     2101 acgacgacga ggggcagttt gccgggcggc tgatggtgct gcacgacgtg acgcaccaga
     2161 agcaattgga ggccgaccgc gacgcattca cgcagatgct ggttcacgat ctgcgcagcc
     2221 cggtctccgc agtcatcagc ggcttgcagc tcatcgagct ggccatccag gagggcgatc
     2281 cacccgatct gctgctgaga agcgcgcggg cctcgctggc cagcgcgaac aagctgctga
     2341 atctgattgg ggcgctgctg gacatccaga agctggagac gggccaactt gagctccagc
     2401 tccaacccgt cgcgccgcct tcgctcgtcc gagaagtggt ggacattctg cgcccgcttg
     2461 cggagatgtc cggcgtgagg ctcgagatgg acgcgcctga agacacgcct ccggtgcagg
     2521 gcgatcccga tcatctgcgg cgcgtattga ccaacctggt ggacaacgcg ctcaagttcg
     2581 tcggcgaagc gggccgcgtg cgtgtgtcgg tcgcggcaga cggcgagttc gtgcgcttct
     2641 cggtcgcgga caacggcccg ggcgtgccgc tcgaataccg cgagcgcatc ttcgggcgat
     2701 acgtgcaagt gcctgggcgc accgcgcggc ggcgcggcac cggcctgggg ctggcatact
     2761 gccggatgat cgtcgagatg cacgggggca aaatctgggt cgaatcatca cccctgggcg
     2821 gcagcgactt catttacacg ctgccggtgg ccgaggctcg cgaatgacga gggacggact
     2881 tacttctgat gaatgttcct cgttcttcga ggacatgatg cacgccgcgc gagcagtcga
     2941 gtgtgtcttc agaccgctga aaatgaactt tgaacttctt ggcaacgccg tgccacattt
     3001 gcacgcgcat atcgtaccgc gctactacgg cgacccggca cctgggagac cacttgaccc
     3061 catggcacaa caggttacac tgacgttgcc agaatatgag gaacggatcc gacagctccg
     3121 agaagccctt ttagcagtgg ggtagagagt ggagtttcct gcttgaaaaa gggcaggcgt
     3181 ggaggcatca gtcgttggca gacgtgattt gtcgaaaggc cgtttcaatg tccgactgat
     3241 gttccacgta gagttgttca gccgcctgac gcatctcttc aatcggcgtc gccgcgacga
     3301 cgctggcaaa ctcgtcgtct atggcaacga caccgatcac tcctgccaat ccatatctcg
     3361 gataggcgac tgccatcgga acggcccaca gcgcgcggat ggggaaaatg atccgtcgag
     3421 gaatgcctgc cagggcgagg ttgccgagat gatcagcgat gaagttctca gcgatgtgaa
     3481 ctgcggcttc ggcggtcagg gttgtcccgc agggagaaac gcagttgagg ccgtccgtat
     3541 agcactcatg cgattctcct cgtgttgaca atctaatgat attatacccc acatgatcga
     3601 tccacatctg gataacaaag tcatcctgat cacaggcgcc aatcacggca tcggcgcagc
     3661 caccgcagaa gcgttcgcgg cacaaggcgc gaaagtcttc atcacctact atcgtggcgc
     3721 gtgccgtcac tcggatgagg aattggccca ggcgcgccag gcgggtatcg gcggtgacgt
     3781 gctcgtcaac aatcacacgc attgcgtccc cgagacgttc gatccggcgt tggcaacgga
     3841 ccaagacggc ggcattcacc ttgtctcggc tgtcgggatt gacgcccact ttgccatcaa
     3901 tgcccgtgcc tacgcgctca tgatgtccga atacctggcg cgttatctga aacggtcggc
     3961 tgaatcaggc cgcatcgtca acgtcagcac ggatgccgct cacgcccaca ccgccaacgt
     4021 cagttacgcg gccagcaaac atgccatcga atcctatagc cgctcggctg cggcggagat
     4081 gggcaggtat ggcatcacca tcaacgtcgt cgcgcccggt ccaattcaga ccggctatgt
     4141 cacgcctgag gccgaggcgc cccttcgccg cgtaggagag cctgaggatg tggcggatgt
     4201 catcgtcttt ctcgcttctg cacaggcgcg atggctgacg gggcaactcc tatacgttgg
     4261 cggtggctgg cgaatggctc agtagtgtcg cctaccgaag ctgctgccaa accgccggga
     4321 gactctccgg cgcaagggga ataaactcct cagtggaaca acctttttgg attgatgaac
     4381 cgtatccctt tttcccctac ggggcatcca aacccggcgc aagggcgagc attctgccgc
     4441 acgccctctt gcttggatgc cagttggtct tcctggcgta catcgccgca gaactgagat
     4501 tccctccagg gggatggata ctgttcgggc tgctatctct tattccattg acaggcatct
     4561 atctgcagct acgccggaag tccttgacgg cccgtgcggc cctcgttcaa cagcgggcgc
     4621 gcgaacagat aggtgcctcg acgatgggca gcgccatcca tgtcgcaggg catcctctgc
     4681 tcgatcgtga gcagcctgtc gtcatagctt tgcgtggtga tcagttgctg ttcttcagtt
     4741 atcaaaatcg caatcccatc gacgttttgc cgattggaga attacaagat atccgcacgg
     4801 tcgtgtatga cgacgatcgg gttccgcatg ttgacgtgat tgacagcacg gcccaggcat
     4861 tgcagttgac attcacctgg cgtggtcaca cctgcaccag tctgtttcgg cgcatgcaca
     4921 aagtccggcc tatcgattgg tatcaggcca ttcaacaagc acgcctgtta tcggtcactc
     4981 atgtcaacgc ggcctgagat tcggctgcac ataatccgtg tgcgcacggg tgttctggat
     5041 caacctctca agttagccac tctcgcacgt catccacaga tacaatcggt ggcttgccca
     5101 gatcatagta ccgcgcactg gaaaactcat acgctgcacg gttagttgcc agttggcatg
     5161 gccacgtagg cagataaggc aaaccgccct cgccaggcca cgccatctcc ttttcgatct
     5221 ccacatcgtt ccgacgcaga tgctcgcgcc aggcgtcgat gtctgcgtgc cgcatcgcga
     5281 acgcgacgtg gcccggcccg cgcgcgccgt gcgtcggtac gctgctcaca ctgtcactcg
     5341 gctggctcgt ggcttcggga ttgaacagca agaagacgcc gtcgccgcag cggaagaaga
     5401 cgtgcctccc caccacgcgc gagatgcgct tcagcccgca gactcgcgtg tagaagtctt
     5461 cggcggcgac gagatcgtcg acgtacaggc aggtctcgag gacgtgtgtt gcttgcatca
     5521 cttccctctc caggcgagcc gctgcagggg tggtcgggag accgctcccg aactcgaaga
     5581 gcgttgccac cactcacaat gagttctaca aacatggcgc tcctgtttac gggcgaacag
     5641 tgtcaacgaa tgggcaacga atcaacgaat cagtgagtca gcgaaacggt gtgttttcac
     5701 gcatcacgtt ttacgcaatc acgcctcggt ctcgaccagc tcctgcat
//
LOCUS       DBNHXB010000278        18972 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3427713, whole genome shotgun sequence.
ACCESSION   DBNHXB010000278 DBNHXB010000000
VERSION     DBNHXB010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 18972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18972
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3427713"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..962
                     /locus_tag="AC162G_11205"
     CDS             60..962
                     /locus_tag="AC162G_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR5076431.1"
                     /translation="MSATTKKTFTIVSLTIVAIVLLSACQPSAPTDLLGEIKARGTIR
                     ISTDPNYAPQSSSVPDATRKADTKCASDELTSAELEGFDIDTAVEIATRLGVEPCFVT
                     PQWEIITAGNWAGRWDISVGSMTITTQRQEILWFTPGYYFTPAQLAARQGSDIDSVDD
                     IAGKPVCVGTATTYESYLSGQDVGIPASDIKVPPPAGVQVFPLDTDAECAQAIRAGRT
                     DFDTFLTSGTVVDEAIAQGIPVVKVGSPVFVENLAVAIDKSSPKDPQSLLDEIGKIIT
                     AMHADGTLKASSIKWFGADLTTAP"
     gene            1185..2024
                     /locus_tag="AC162G_11210"
     CDS             1185..2024
                     /locus_tag="AC162G_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR5076432.1"
                     /translation="MATQVAIRPTLAQQILQQQAQAQRVFAVKVIISWAALLAFLLFM
                     FSSVTIQIGPIAIRTIQLDRAFIDKYWRYISEGAIETLRICFLSIVFASVLALLAALG
                     RLSRIAPIYALSTFYVSLIRGTPLLLQIFFFFLALPQMGIVLSGLVAGVVALSLNYGA
                     YMSEIFRAGIQSVGKGQREAALALGLTQGQTMRRIVLPQALRVVIPPVGNEFIAMLKD
                     SSLVAMTGFVREIMWRAKDVGQSHFRGLEALLVAALWYWVMTLIFSAIQSRLETRLAR
                     GDR"
     gene            2033..2800
                     /locus_tag="AC162G_11215"
     CDS             2033..2800
                     /locus_tag="AC162G_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR5076433.1"
                     /translation="MIQVTDLHKYFGPLHVLRGISLSVERAEVVCVIGRSGSGKSTLL
                     RCINFLEQPTHGRIEVDELVVNVERHRLTPEQKLVIRHIRMRTGMVFQEFNLFPHLSV
                     IDNLIEAPVTVKGMKKADAIPLAEKYLAKVGLMHKRDEYPARLSGGQKQRVAIARALT
                     MQPEVMMFDEPTSALDPELIGEVLDVMKELAREGTTMLVVTHEMGFASEVCNRVIYMD
                     DGQIVEEGPPKLIFTSPADPRTRQFLDRLLRTEASEK"
     gene            2908..3660
                     /locus_tag="AC162G_11220"
     CDS             2908..3660
                     /locus_tag="AC162G_11220"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF039614.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016403 - dimethylargininase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylarginine dimethylaminohydrolase family
                     protein"
                     /protein_id="HIR5076434.1"
                     /translation="MIPTYAIVRPPGDTYPDSLTRLDPPPPVDLKLARRQHAAYDAAL
                     RECGLEVIALPPDPEHPDSVFVQDPVLVVDGRAIICRSAAESRRGEADALVGVLRPYM
                     DIVELQPPATLDGGDVLIADRRLYVGLSTRSNRAACEQLGEITGWPVEGVPLPENLLH
                     LLSGCTYLGANRLLVVDSLAPLMPGFEHILVPGDEEQAANVLIAGHHAIVPAGYPKTA
                     ALVAQCGFEIHLAPMSEYEKRDGGVTCSSLLF"
     gene            3673..3873
                     /locus_tag="AC162G_11225"
     CDS             3673..3873
                     /locus_tag="AC162G_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076435.1"
                     /translation="MRNDERKLQEEFDTVGLCRTCRHVKVIESARGSTFYLCRLSETD
                     PRFPKYPRLPVVRCAGYEREQK"
     gene            complement(3895..4635)
                     /gene="pgl"
                     /locus_tag="AC162G_11230"
     CDS             complement(3895..4635)
                     /gene="pgl"
                     /locus_tag="AC162G_11230"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR5076436.1"
                     /translation="MKPEIVVLADLSAVANEAAGRIVRLARESIAAQGRFTIALSGGS
                     TPRALYVLLASKEYRDRIEWDRALVFWGDERCVPPDHADSNYRLAHETLLSKVSIPSG
                     SVHRVMGEADPEHAAMLYEQIVRREVPARDGVPAFDLILLGMGPDGHTASLFPGTTAP
                     RETHRLVAANDVPQLNAHRITFTVPLINAAANIMFLVAGGDKADALRAVLEGEFKPDV
                     FPAQMVRPANGQLMWLVDRAAAVHLRNK"
     gene            complement(4641..5936)
                     /gene="tal"
                     /locus_tag="AC162G_11235"
     CDS             complement(4641..5936)
                     /gene="tal"
                     /locus_tag="AC162G_11235"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HIR5076437.1"
                     /translation="MTTNRVQELAKLGQSIWYDNISRKLLTSGRLHELVDEGLLGMTS
                     NPTIFEKAIAGSRDYDGDLRRLVASGGSVEAIYDALTISDVSAAADELRRVYDRTNGR
                     DGYVSIEVAPTLANDAVGTIADARRLWTTLGRPNILVKVPATRPGLGAIEQLLSEGIN
                     VNVTLMFSMTHYEAVTEAHIRGLEARAAAGRPVERVASVASFFVSRVDTMVDAMLEAI
                     VRDEGADAGLAASLMGKSAVANSKLVYERFRQIYDGPRFAGLKARGARLQRVLWASTS
                     TKNPNYPDTLYVDTLIGPDTVNTVPPETYDAIQDHAFVRRSVDQDMDAARKVVSDLAR
                     LGIDLNQVGERLSAEGVEKFTQSYEQLLSTIATKRDALLAEMTPAQTGATKRKPAAKK
                     KAARKQLVRRKAAAAKPAAKKMTGRKPAAKKKTTVRRKR"
     gene            complement(5950..6408)
                     /locus_tag="AC162G_11240"
     CDS             complement(5950..6408)
                     /locus_tag="AC162G_11240"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HIR5076438.1"
                     /translation="MRIAMGADHGGFDLKNELAAHLKTSGHDVVDLGAHAYDAGDDYP
                     DFTRPVAEAIANGEAERGIIVCGSGVGASIAANKIRGVRAALCHDTYSARQGVEHDDM
                     NVLCLGARVIGVELARELAAAFVGARFSGEERHVRRLNKVLAIERDATRS"
     gene            complement(6475..7362)
                     /locus_tag="AC162G_11245"
     CDS             complement(6475..7362)
                     /locus_tag="AC162G_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012982.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5076439.1"
                     /translation="MYLIRPWLYVGKFNETQDDDLLRRREIGAMLQLADSVKHEGIPS
                     LYVPVMDGEPLPHDMLRIGVEFVRLEKALGRNVLIACGAGISRSAAFTVAALKEEEGL
                     SLLDAFREVRARHPHAVPHAALWESMCEYYREDVPYAKVLQVIWPELEQGEKSMPRPG
                     HPKYHTVGEIMTTEVATVPASMPVEDALRLMRQRGIHSVIVEPEQPGGAYGIMTQRDL
                     LRKIVAADRPLLNVAVRDLMTSPLITVSPDTTIRQCSIIMLDANIRRAAVMKDGKLAG
                     IISDTDIFQAVEERGWGPD"
     gene            complement(7389..7730)
                     /locus_tag="AC162G_11250"
     CDS             complement(7389..7730)
                     /locus_tag="AC162G_11250"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015184981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HIR5076440.1"
                     /translation="MQRDLAYLSDILQAARLVQAGTQGITESEFSDDWMRQSAIIRQL
                     EIIGEATKHLSREFKASHPEISWKSVAGMRDVLIHAYASVDLKEVWKAAIRDIPQLIR
                     VIEPLLPLSGE"
     gene            complement(7720..8034)
                     /locus_tag="AC162G_11255"
     CDS             complement(7720..8034)
                     /locus_tag="AC162G_11255"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015147995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5076441.1"
                     /translation="MNNLKINLPEEKVASFCRRWKITELALFGSVLRDDFGPDSDVDV
                     LVTFAPDAPWSLVDHVTMQDELKQILQRNVDLISRRGLEQSHNSIRRKAILSSAQVFY
                     AA"
     gene            complement(8178..9506)
                     /locus_tag="AC162G_11260"
     CDS             complement(8178..9506)
                     /locus_tag="AC162G_11260"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR5076442.1"
                     /translation="MTDVPLASGSLSYTSEAQACLSAARVEAQARHAPQVEPEHLLMA
                     LLTPASGAVWNALVGTLGDPAPLRKRADAALSSPPIESGTGEPPPSHRLERVLRDAGD
                     AAKSSGAPVDTPHLLVGLLDEGGAAANILRQAGIDARRLRLWLRKPPEVSQTTPPSAT
                     LRGQARPPVETLRKTRAQPALDTLPLRQALPRLIDWRAVLALLAVLAIGAWLATQPDY
                     GTAGAVFLVVGGWIVSLCAHEFAHALAADLGGDYTARQKGYLSFNPLAYTHPLLSIVM
                     PLLFLLLGGFGLPGGAVYVETHRLRSPRWSSIVSAAGPLASMLMALIFALPFLFSLVS
                     GLQIYLEPTLWGALAAVAMLNVSGVLFNLLPIPPLDGFGIIAPWLPPELRLRLSMFGF
                     LGLWLVILAFWTIEPLSQSFWNAVYSILEIFRIDPGLAAYGISRFMFWRS"
     gene            complement(9561..11120)
                     /locus_tag="AC162G_11265"
     CDS             complement(9561..11120)
                     /locus_tag="AC162G_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076443.1"
                     /translation="MTSASLSPSHAARRINRVEMRGIVKRFPGVIANAGVTFDASAGE
                     VHALLGENGAGKSTLMRQLYGLYQPDEGEILIDGRPHVFRSPADAIRAGIGMIHQHFM
                     LVPTLTVAENVALGLRSSREPLLDLDKVSARIRALAQAYGLKVDPNATIWQLAVGEQQ
                     RVEIVKALYRGASLIILDEPTAVLTPQEVDDLFVTLKRMAAEGHALIFISHKLHEVMS
                     ISDRVTVLRDGRVVGAHHTRSVTGSQLAQMMVGRELKPLAAAARESRAGDVRLKVESL
                     RAQGDRGTEMLRGVGLEVRAGEIVGVAGVSGNGQRELAECLVGLRRPTTGRVYLDDRD
                     ITEASAQARMAAGQAYIPEERMRDGVIRDFTVQENLFLRDHAARDYTRGIFMNFGAMR
                     VRARDLITRFNVKTPRLDTPVKNLSGGNIQKLILARELSRQPKVLIASQPTRGVDIGA
                     TEYIHRRLLEQRDAGTAILLVSEDLEEIQALSDRIAVMYEGQIVGIADRGQATPQELG
                     LLMAGITRTSV"
     gene            complement(11218..12219)
                     /locus_tag="AC162G_11270"
     CDS             complement(11218..12219)
                     /locus_tag="AC162G_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HIR5076444.1"
                     /translation="MTLRRFWILLLLVALLASACGGGAVTTAPAAAQPFRIAVVMPSA
                     TNDMAFSQSMYSALLAVQAEMGGPSALEIAYSEGMFNVPDAAAALRDYASKGYDLVIG
                     HGSQYGSSVQEIAPDFPDTSFAWGTDVNTFGLPNVFAYTAAAEEGGYVNGVLAAELTE
                     SEKIGVTGPIEVGDAKTYIDGFVQGVEATDPDVEVAKTWTGSFSDVALMAEAAKTHIA
                     AGADALTGSSQSVVGSIGAAKEAGGVLWFGTQQDQASLAPELVVASQVYDWTPFIKEM
                     IEKHKAGTLGGEVFTLRLANDGLKIAFNPQYDLPPAVKAAADAAIEGIKNGTIKVEP"
     gene            complement(12328..13260)
                     /locus_tag="AC162G_11275"
     CDS             complement(12328..13260)
                     /locus_tag="AC162G_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076445.1"
                     /translation="MSDLFTLPVFIGVLTSAIRLATPYLYASIGETFAQLSGVLNLGV
                     DGIMLMGAFSGFYVALNTGDLWLGMAAAAVVGLLMGLLMSVISVTLKAEQGISGIGLY
                     LFGLGLSSLLYKEAIGTVKTIDGFPTVKIPVLGDIPIVGEIVFQQSLPVYGAFLLVPL
                     AWLFLNRTTWGLKIKSVGQNPAAADSLGVSVNAVRYFCVCLGAILAGIAGASLSLALL
                     NLFQENLTAGLGFIAVALVYFGGWRPLGILAGALLFSTVNALQLWMQVLGFQIAPELA
                     VMLPYILTIAVLAVTVSRVRQPAALNKPFERGEG"
     gene            complement(13253..14494)
                     /locus_tag="AC162G_11280"
     CDS             complement(13253..14494)
                     /locus_tag="AC162G_11280"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076446.1"
                     /translation="MNEPMLDRVASAPRRIRAPRFDWRDVALRALTPILAVLAAMLVG
                     AILLLLLNVNPIEAYAAMIGGALGDVSGLTQSVVKATPLLLVALGIVIAFRASVINIG
                     GEGQIILGALAGTWFSLTFRMWPSWLLIPTTMGVGFLAGAFWGFIPGILKARLNVNEI
                     LSTVMLNAIALQLMNYLLRGPMIDPAGVAAGTYLAQSERLPDSVWLPRLVPQTLLHTG
                     AILAVILAVLVYVFLWRTTIGYRIRAVGLNPDASRYAGIRVPFHQALALTLAGGFAGL
                     AGVIEVIGVQHRLLQGITSGYGFTGIVAALFGGLHPIGAIPASFLFGVLLVGADKMQR
                     AMQVPSALIGTLLGLIVLFVVGSAYLSRRWAARRAIEVAAEEDMPAEDGTLPAEGASA
                     GPVRHSTAACAPPSGVEPSHE"
     gene            complement(14600..15541)
                     /locus_tag="AC162G_11285"
     CDS             complement(14600..15541)
                     /locus_tag="AC162G_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076447.1"
                     /translation="MEAALASILLASAPLIFATIGEAITEKSGVINLSLDGSILLSAM
                     IGFAAAYLTGNVWLGFVAAAIVSLLVALLVAYASISLRLNQVAVGFVLTLLCADVSSF
                     LGNPFVRIQGPSVPHMPIPILEDIPFLGPILFDQDLVVYASILTIIAAYLFVFRTRRG
                     LELQGMGERPEAAHARGVPVNRMRYVYAAVGGALVGIAGAAYSLDVKLGWSYRHTAGL
                     GWIALAIVIFGGWHPVRVAFGAYLFGALQVLALKLQPVLPNLSQVLSILPFPLMIFTL
                     LLVSSDSIGRLLDRWPTVRGVLRSEPPSALGTHFEQE"
     gene            complement(15663..16688)
                     /locus_tag="AC162G_11290"
     CDS             complement(15663..16688)
                     /locus_tag="AC162G_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076448.1"
                     /translation="MITLRRVGLGLLPVVLAVALALVLLLLVGAAPLQAFQHLIEGAF
                     GTPEKISDTLMAWVPLVLCASGLLITFAAGLWNIGVEGQIVFGAIAAAWVAREVKTPA
                     EVLVPLTMIGGIAGGVVWGLLVGVLRTFGKVNEIFGGLGLNFVAIGLTIYLIIGPWKR
                     AGIASTSGTDTFPREAWLPTLEGLRLAPLAIVLAIAAVIVVYVLLRGTLFGLQLKAVG
                     KNMRSAFLLGIPTHRYMLIAFALCGGFAGLAGAIQATGFHHKLVPAVSGGYGYLGILV
                     ALLASFRAAWIAPIALFFAMITVGSTQLQLRLNLDSSLGGVLQGMLVLFVLLARGWQA
                     KRVISNQ"
     gene            complement(16765..18231)
                     /locus_tag="AC162G_11295"
     CDS             complement(16765..18231)
                     /locus_tag="AC162G_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076449.1"
                     /translation="MRVELRDIHKHFGPVRANDGVTLTVEAGTLHGLLGENGAGKSTL
                     MKILSGFQPADSGEIVLDGKPARIRSPVDAIRHGIGMLHQDPLVFLPFNVIDNFLLGS
                     PGPLRLDRASAERGLKRLCDQFGFVLDPDMPARALTVGERQQLEIVRLLWLGARVLIL
                     DEPTTGISAPQRVKLFETLRALAAQGMSVIFVSHKLEEVEELCGKVTVMRRGKVVGEA
                     GLPCPVERLVEMMFGQVVVPGGRLPVKQGEPVLQLDHVSLRDRLLSINDLSLSVNAGE
                     VIGLAGLEGSGQRTTLRACAGLLRPSAGRVHVGGRDLTGHSYREFLSAGVHYLPAGRL
                     EEGLVAGMTLAEHFVLASGDRRFFVDWAGARQRAEETIQFFEIMGRPTSTAEALSGGN
                     QQRLLLAMLPPKLRLLLMEHPTRGLDVESANWVWMQLLARRAHGTAIVFASADLDELL
                     QYSDRIMVFFAGRVLNVLNASETDVEQLGHLIGGKQVA"
     gene            complement(18343..>18972)
                     /locus_tag="AC162G_11300"
     CDS             complement(18343..>18972)
                     /locus_tag="AC162G_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR5076450.1"
                     /translation="KYCWENYRGNDPADLKFEVTWIGFWFNIPGVTLDPTQVANEFFD
                     GGADVVISGIDTTEGLVVAGQRAAADETVWAVPYDFEGACEEAPFICLGVPYFNWGPA
                     YLRVAQAVIDGSFEATFEWNGPDWTDINNPDTTAVGFVKGPALSEDNSATLDEFISGL
                     ADGSIVLFKGPLNYQDGSTYLEDGEVATDLQVWYMPQLLEGMLGASTSQ"
BASE COUNT         3593 a         6193 c         5887 g         3299 t
ORIGIN      
        1 cacgaaacac gatcaggcta cccaccgacg catgcacatt ctctcaggag gagatacaca
       61 tgtccgctac aacgaagaag acgttcacca tcgtcagctt gacgatcgtc gccattgtgc
      121 tcttgagtgc gtgccagccc agcgcgccga ccgacctgct tggcgagatc aaggcgcgcg
      181 gcacgatccg catctcgacc gacccgaact acgcgccgca gtcgtcgtcg gtccccgatg
      241 cgacacgcaa ggccgacacg aagtgcgcct ccgacgagct gacatccgct gagttggagg
      301 gcttcgacat cgacaccgcc gtcgagatcg ccacgcggct gggcgtcgag ccgtgctttg
      361 ttacgccgca gtgggagatc atcaccgccg gcaattgggc cggccgctgg gacatcagtg
      421 tcggctcgat gacgatcacc acgcagcgcc aggaaatatt gtggttcacg cccggctact
      481 acttcacgcc ggcgcaactg gcggcccgcc agggttccga catcgattcc gtcgacgaca
      541 tcgcgggcaa acccgtgtgt gtcggtacgg ccacgacgta tgaatcgtac ttgagcggcc
      601 aggatgtcgg catccccgca tccgacatca aggtgcctcc gccggccggc gtgcaggtat
      661 tcccgctgga caccgacgcc gagtgcgctc aggccatccg ggccggccgc accgatttcg
      721 acacctttct gacttcgggc accgtggtcg acgaagcgat cgctcaaggc atcccggtcg
      781 tgaaggtcgg ctcgccggtg ttcgtcgaga acctggccgt cgcgatcgac aagagctcgc
      841 ccaaggaccc gcagagcttg ctggatgaga tcggcaagat cattacggcg atgcacgccg
      901 atggcacgct gaaggcctcg tcgataaagt ggttcggcgc cgacttgacg acggcgccgt
      961 agacgacgag cgtggccgga gtggaggcgg cggtgcagtc gatggccgcc gcttccgcac
     1021 cttgatctgg cgcatgacgc tcgcgtcgat cggcgacagt cagtgcctgt ccgaaaaccg
     1081 gcggacttgg cttccatgcc cagccatccg gcggcgtgtc cgcttcgctc cggggtgctg
     1141 cgcaaggagc cacctccgca gacttttcct agggaggtgg aacgatggcc acccaagtag
     1201 caatccgtcc gacgctggct cagcagattc tccagcagca agcccaggcg cagcgcgtgt
     1261 ttgcggtcaa ggtgatcatc tcgtgggcgg cgctgctggc gtttcttctg ttcatgttta
     1321 gcagcgtgac catccagata ggcccgatcg ctatccggac catccagctc gaccgagctt
     1381 tcatcgacaa atactggcgg tacatcagcg agggcgcgat cgagacgctg agaatctgct
     1441 tcctgtcgat tgtcttcgcc agcgtcctgg ccttgctggc tgcgctaggc cgcctgtcgc
     1501 gtattgcgcc catctacgcg ctgtccacgt tctacgtctc gctcattcga ggtacgccgc
     1561 tgctgctgca aatctttttc ttctttctcg cgctgccgca gatgggcatc gtgctctcag
     1621 gcctcgtcgc cggggtggtg gccctctcgc tgaactacgg cgcgtatatg agtgagattt
     1681 tccgcgccgg cattcaaagc gtgggcaagg gccagcgtga agcggcgctg gcgctgggcc
     1741 tgacacaggg ccagaccatg cgccgcatcg tgctgcccca ggcgctgcgc gtcgtcatcc
     1801 cacccgtcgg caatgagttc atcgccatgc tgaaagattc ttcgctggtc gcaatgaccg
     1861 gatttgtgcg cgagatcatg tggcgagcca aagacgtcgg ccagtcgcat tttcgcgggc
     1921 tagaggcgct gctcgtcgcc gcgctgtggt actgggtgat gacgctgatc ttctccgcca
     1981 ttcagtcgcg gctcgagaca cggctcgcgc gcggggaccg ctaagcatgc cgatcatcca
     2041 ggtgaccgac ctgcacaagt atttcggtcc cctgcatgtc ctcagaggca tctcgttgtc
     2101 tgtcgagcga gccgaggtcg tctgcgtcat cggccgatcg ggatcgggca aatcgacact
     2161 gcttcgctgc atcaactttc tggagcagcc aacgcacggg cgaatcgagg ttgacgagct
     2221 ggtcgtgaac gtcgagcgcc accgtctcac gcccgagcag aaactggtga ttcgccacat
     2281 ccggatgcgc accggcatgg tgttccagga gttcaacctg tttcctcatc tgagcgtgat
     2341 cgataacctg atcgaagcgc cggtcacagt caaaggcatg aagaaggcgg atgccatccc
     2401 gctggcagag aaatacctgg ccaaggtcgg cctgatgcac aagcgcgacg aatacccggc
     2461 gcgtctgtcc ggcggccaga agcagcgcgt cgctatcgcc cgggcgctga ccatgcagcc
     2521 agaggtcatg atgttcgacg agccgacctc ggcgctcgat ccggaactga tcggcgaagt
     2581 gctcgacgtg atgaaggagc tggcccgcga agggacgacg atgctggtgg tcacgcacga
     2641 gatgggcttc gcgagcgagg tgtgcaatcg cgtcatttac atggatgacg ggcagatcgt
     2701 tgaggaaggc ccaccgaagc tgatcttcac gtccccggct gacccgcgca cccgccagtt
     2761 cctcgatcga ttgctgcgca ccgaggccag tgaaaagtga tcagtaatca gtaaacagta
     2821 atcagtgatc ggtgatcggt gatcagtgac aagagccaac ggcgtcatgt gtcactcatc
     2881 acacatcact tgccggcacg aggaaggatg attcctacct acgcgattgt ccgcccaccg
     2941 ggcgatacat acccggactc gctcacacgg ctcgatccac cgccaccggt cgatttgaag
     3001 ctggcgcggc ggcagcacgc ggcgtatgac gcggcgctgc gcgagtgcgg cctggaggtc
     3061 atcgccctgc cgcccgatcc cgagcatccc gattctgtct tcgtgcagga ccccgtcctg
     3121 gtggtggatg gccgcgcgat catctgccgt tcggctgccg agtcgcggcg aggcgaagcc
     3181 gatgcgctgg tcggagttct gaggccgtat atggatattg tcgagctaca gccgcccgcg
     3241 acgctcgacg gcggcgacgt gctgattgcc gaccgccgcc tgtacgtcgg attgtcgacg
     3301 cgatcgaatc gcgcggcgtg cgaacagttg ggagagatca cgggctggcc ggtcgaaggc
     3361 gtccccttgc cggagaacct tctgcacttg ctcagtgggt gcacttatct cggcgcgaat
     3421 cgcttgcttg tcgtcgattc gctcgcgccg ctcatgccgg ggttcgagca tattctcgtg
     3481 cccggcgacg aggaacaggc ggccaacgtg cttatcgccg gccatcatgc tatcgttcca
     3541 gcaggatatc ccaagacggc cgcgctcgtc gcgcagtgtg gcttcgagat tcacctcgcg
     3601 ccgatgagcg aatacgaaaa gcgcgacggc ggggtgacgt gctcgtcgct gttattttga
     3661 ctgcgaaacg gtatgagaaa cgacgagcgg aagctgcagg aggagttcga tacggttggc
     3721 ctgtgccgca cgtgccggca cgttaaagtg atcgaaagcg cgcgcggcag cacgttctat
     3781 ctgtgccggc tgtcggagac cgacccgcgc ttcccgaagt atccgaggct gccggtggtt
     3841 cggtgcgcgg ggtacgagcg agaacagaag tgaccagccc ttacacggtg ccggtcactt
     3901 gttgcgcaga tgcacggccg ctgcccgatc gaccagccac atcaactgcc catttgctgg
     3961 cctgaccatc tgcgccggaa acacatccgg cttgaattcg ccttcgagca cggcgcgcag
     4021 cgcatccgcc ttgtcgccgc cggcgacgag aaacatgatg ttcgcggcgg cgttgatgag
     4081 cggcacggtg aacgtaatgc gatgggcatt gagctggggc acatcattcg ccgcgacgag
     4141 ccggtgtgtt tcgcgcgggg ccgtcgttcc aggaaatagc gaggcggtgt ggccgtccgg
     4201 tcccatgccg agcaagatga ggtcgaaagc cggaacgccg tcccgagccg gcacttctcg
     4261 ccgaacgatc tgctcgtaga gcatcgcggc atgttccggg tcggcctcgc ccatcacgcg
     4321 atgcacactg cctgaaggga tggagacttt agaaagcagc gtttcgtgtg cgaggcgata
     4381 gttgctgtcg gcgtgatcgg gcggcacgca gcgctcgtcg ccccagaaga cgagcgcccg
     4441 atcccactcg atccggtcgc gatactcttt gctcgccagc agcacgtaca acgcgcgcgg
     4501 cgtcgacccg ccggacagtg caatggtgaa tcgtccctgc gcggcgattg actcgcgcgc
     4561 cagcctgacg atccggccgg ccgcctcgtt tgcgacggcg ctcaggtccg ccagcacgac
     4621 gatttccggt ttcatgctgc tcacctcttt cggcgcacag tcgttttctt cttcgcggcc
     4681 ggcttccgcc cggtcatttt cttggcggca ggtttcgcgg cggctgcttt tctcctgacg
     4741 agctgcttcc tggccgcctt tttcttcgcc gcgggcttgc gcttcgtcgc gcccgtctgt
     4801 gcaggtgtca tttcggccag cagcgcgtcg cgctttgtcg caatcgtgga aagcaactgc
     4861 tcgtacgact gggtgaactt ctccactccc tcggcagaga gccgctcgcc gacctgattc
     4921 aagtcaatgc cgagccgcgc caggtccgac acgaccttgc gcgcggcgtc catgtcctgg
     4981 tcgacactgc gccgcacgaa ggcgtgatct tgtatcgcgt cgtacgtctc cggcgggacg
     5041 gtattcaccg tgtcggggcc gatgagcgta tcgacgtaca gcgtgtcggg gtagttcgga
     5101 ttcttagtgc tggtgctggc ccacaggacg cgttgcaggc gcgcaccgcg cgctttcagc
     5161 cccgcgaagc gcgggccgtc gtaaatctgc ctgaaacgct cgtacaccag cttggagttg
     5221 gcgactgccg atttgcccat gagcgatgcg gccagtccgg cgtctgcccc ctcgtcgcgg
     5281 acgatcgctt cgagcatggc gtcgaccatc gtgtcgacgc ggctgacgaa gaacgacgcg
     5341 accgaggcga cgcgctcgac cggccgtccg gctgccgcgc gcgcttcgag gccgcgaatg
     5401 tgcgcctcgg tgacagcctc gtagtgtgtc atggagaaca tcagcgtcac gttgacgtta
     5461 atcccttcgc tcaacaactg ctcgatcgcg ccgaggccgg gccgcgtggc cggcactttg
     5521 accaagatgt tgggacggcc gagcgttgtc cacaatctgc gcgcgtcggc gatcgtgccc
     5581 acggcgtcgt tcgccagggt cggcgccact tcgatgctca cgtagccgtc acgcccattc
     5641 gtccggtcgt atacgcggcg cagctcgtcg gctgcggccg acacgtcgga gatcgtcagc
     5701 gcatcgtaga tcgcctcgac gctcccgccg ctggcgacca gccggcgcag gtcgccgtcg
     5761 tagtcacggc ttccagcgat cgccttttcg aagatagtgg ggttagacgt catgcccagc
     5821 aagccttcgt cgacaagctc gtgcagcctg cccgacgtga gcagcttgcg gctgatgttg
     5881 tcgtaccaga tgctctggcc cagcttcgcc agttcctgta cacgattggt cgtcatgcgg
     5941 gtccccggtt caggatcgcg tcgcgtcgcg ctcgatggcg agcactttgt tcaagcgccg
     6001 gacgtgccgc tcttcgccgc tgaagcgcgc gccgacgaac gccgccgcca gctcgcgcgc
     6061 cagctcgacg ccgatgacgc gcgcgcccag gcacagcacg ttcatatcgt cgtgctcgac
     6121 gccctggcgg gccgagtacg tatcgtggca taacgcggcg cgcacgcctc ggattttgtt
     6181 cgcagcaatc gacgcgccta ccccgctgcc gcatacgatg atgccccgct ctgcctctcc
     6241 gttcgcgatc gcctcggcca cgggacgggt gaagtcggga tagtcgtcac cggcgtcgta
     6301 agcatgcgcg ccgagatcaa ccacgtcgtg cccgctcgtc ttcaaatgcg cggcaagctc
     6361 gttcttcaaa tcgaacccgc cgtggtctgc tcccatcgca attcgcaccg cattctcctg
     6421 ggtcatttgg acgccgatgc tgcggatgaa ggccgccgtg tccgtgttcc gagatcaatc
     6481 cggtccccag ccgcgctctt cgactgcctg aaagatgtcc gtgtcgctga taatgccggc
     6541 gagtttgccg tccttcatca ccgccgcgcg gcgaatgttc gcgtcgagca tgatgatcga
     6601 gcactggcga atcgtcgtgt ccggcgagac ggtgatcagg ggtgaggtca ttagatcgcg
     6661 cacggcgacg ttgaggaggg gccggtcggc ggcaacgatc ttgcgaagca ggtctcgctg
     6721 ggtcatgatg ccatacgcgc cgccgggctg ctccggctcg acgatcacgc tgtgaatgcc
     6781 tctctgccgc atcaggcgca gcgcgtcctc gaccggcatg gacgccggca cggtggcgac
     6841 ctcggtggtc atgatctcgc cgacggtgtg atatttcgga tggccgggac ggggcatgct
     6901 cttctctcct tgttcaagct cgggccatat gacctgaagc accttcgcgt aagggacgtc
     6961 ctcccgataa tattcacaca tcgattccca cagcgccgca tgcggcacgg catggggatg
     7021 cctggcccgc acctcgcgga acgcgtccag caggctcaag ccctcttctt ctttgagcgc
     7081 ggcgacggtg aacgccgccg agcggctgat gcccgcgccg cacgcgatga gcacgttgcg
     7141 accgagcgcc ttctccaggc gcacgaactc gacgccgatg cgcagcatat cgtgcggcag
     7201 cggctcgccg tccatgaccg gaacgtacag cgacgggatg ccctcgtgct tcacgctgtc
     7261 ggccaactgc agcatcgcgc cgatctcgcg gcgacggagc agatcgtcgt cttgcgtctc
     7321 gttgaacttc ccgacgtaca gccaggggcg gattaggtac atagaagtcg aaagtctgcg
     7381 cctcgcagct attcgccgga tagcggcaat aagggctcga tcaccctgat caattgagga
     7441 atgtcccgga ttgccgcttt ccagacttcc ttcaaatcga ccgaagcata agcatgtatc
     7501 aagacgtcgc gcataccggc gacgcttttc caggagatct ccggatggga tgccttgaac
     7561 tccctggaaa gatgcttggt tgcttcgccg attatctcca attgccggat gatcgcggac
     7621 tggcgcatcc agtcgtcgga gaactcggac tcggtaatgc cctgggtgcc ggcttgcacg
     7681 aggcgcgccg cttgcaagat gtcggacaga taagcaagat cacgctgcat agaacacttg
     7741 tgccgagctc agaattgcct tccggcgaat cgagttatgg ctctgttcga gtccccggcg
     7801 gctgatcagg tccacatttc gctgcaagat ttgcttcagt tcgtcctgca tggtgacatg
     7861 atccaccagg ctccacggcg cgtcgggtgc aaaggtgacg agtacatcga catcactgtc
     7921 tggtccgaaa tcgtcacgta gcacagaacc gaacagggca agttctgtga tcttccagcg
     7981 tcggcaaaaa ctggcgacct tctcttcagg cagattgatc ttgagattgt tcatcgtcgc
     8041 tctccgcacg accgtcaaga gagtccaata aagaataacc gactctgacg gaacgcgtat
     8101 caggcgagag tgtctgccac cgcctcgcgt ccgacgcgag acaaccgtcg gttgaaactg
     8161 aactgattgt aacacgatca gctacgccag aacatgaatc gcgagatgcc ataggcggca
     8221 agccccgggt caatgcggaa tatctcaaga atggaatata ccgcgttcca gaaagattgg
     8281 ctcagcggct caatggtcca gaaggccagg atcacgagcc acagccccag aaagccgaac
     8341 atgctgagcc gcaggcgcag ttcaggcggc agccagggcg cgatgatgcc gaacccgtcc
     8401 aacggcggaa tgggcagcag gttgaacaac acgcctgaga cattcagcat ggcgacggcc
     8461 gccagcgcgc cccacagcgt cggctccaaa tagatttgga ggccgctgac cagcgagaac
     8521 aggaagggca gtgcaaagat taacgccatg agcatcgacg caagcgggcc ggccgccgag
     8581 acgatgctgc tccagcgggg gctgcgcagc cggtgagtct cgacgtatac tgcgccgccc
     8641 ggcagcccga atcctccaag cagcaggaac agcagcggca tcacaatgct gagcagggga
     8701 tgggtgtacg caagcgggtt gaaactgagg taacccttct ggcgggcggt gtagtcgccg
     8761 ccgagatcgg cggccagcgc gtgcgcgaat tcgtgcgcgc acaggctgac gatccagccg
     8821 ccgacgacca ggaaaaccgc gcctgccgtc ccgtagtccg gctgcgtcgc cagccaggcg
     8881 ccgatggcaa gcacggccag cagcgccagc acggcgcgcc agtcgatcaa acggggaagg
     8941 gcctgccgca gcggaagtgt atccagtgcg ggctgcgcgc gtgtcttccg gagtgtttcg
     9001 accggcggtc tcgcctgccc tcgcagcgta gcggaggggg gcgtggtctg cgagacctcg
     9061 ggtggttttc tgagccacag ccggagccgg cgcgcatcga tcccggcctg gcgcagaatg
     9121 ttggccgcag cgccgccctc gtcgagcagc cccacgagca gatgcggggt gtcgaccggc
     9181 gctcccgacg acttcgccgc atctcccgcg tcgcgcagca cgcgttccag gcgatgactg
     9241 ggcggcggct cgcccgtgcc cgactcgatc ggcggggacg atagcgccgc atcggcgcgt
     9301 ttgcgcagcg gcgccggatc gccgagcgtt cccaccaacg cattccacac cgcgccggac
     9361 gcaggtgtga gcagtgccat cagcaggtgc tccggctcga cctgcggcgc gtggcgcgcc
     9421 tgcgcctcga cgcgggcggc gctcaggcat gcttgagctt cagatgtata ggatagagaa
     9481 ccggaagcta aaggcacgtc agtcatacgc ccctttcagc ggtatttcgc tggtcctggc
     9541 tcatacgcgt ttcgtgcgat tcaaacgctg gtgcgcgtga tgcccgccat cagtaggccc
     9601 agttcctgcg gcgtcgcctg gcctcgatcg gcgatgccca cgatctgccc ttcgtacatc
     9661 acggcaatcc ggtccgaaag cgcctggatc tcttcgagat cttccgagac gagcaggata
     9721 gccgtgccgg catcgcgctg ttcgagcaga cgtcgatgga tgtactctgt tgcgccgatg
     9781 tccaccccgc gcgtcggctg cgaagcgatc agcactttcg gctgccgcga cagctcgcgc
     9841 gccaaaatca atttctggat attgccgccg gacaagttct tcacgggtgt gtccaggcga
     9901 ggcgtcttga cgttgaaccg ggtgatcaga tcacgggcgc gcacgcgcat cgccccgaaa
     9961 ttcatgaaga tgccgcgcgt gtaatcccgc gcggcatggt cgcgcaggaa caggttctcc
    10021 tgcacagtga aatcgcggat cacgccgtcg cgcatgcgct cttctggaat gtaggcctgt
    10081 ccggcggcca tccgcgcctg cgccgaggcc tccgtgatgt cgcgatcatc caggtagacg
    10141 cggccggtgg tcggtctgcg cagaccgacc aagcactcgg ccaactcgcg ctgcccgtta
    10201 ccggagacac cggccacgcc gacgatctct ccggcgcgaa cttccagacc gacgccccgc
    10261 agcatttcgg tgccgcggtc accttgcgcg cgcagcgact cgaccttcag tcgcacgtct
    10321 ccggcgcgtg attctcgcgc agcggccgcg agcggcttga gctcacggcc gaccatcatt
    10381 tgcgccaact ggctgccggt gacggagcgc gtgtgatgcg cgccgaccac gcgcccgtcg
    10441 cgcaggactg tcacacgatc gctgatcgac atgacctcgt gaagcttgtg cgagatgaaa
    10501 atcagagcgt gaccctctgc ggccatgcgc ttcaacgtga caaacagatc gtccacctcc
    10561 tgcggcgtca ggaccgccgt cggctcgtcg aggatgatca ggcttgcgcc ccgatacagc
    10621 gccttcacga tttcgacgcg ctgttgttcg ccaacggcca gctgccagat ggtcgcattc
    10681 ggatccacct tgaggccata cgcctgtgcc agcgcccgga tgcgcgccga caccttgtcg
    10741 agatcgagca gtggctcgcg cgacgagcgc aatcccagtg cgacgttctc cgccacggtg
    10801 agcgtcggca cgagcatgaa atgctggtga atcatgccga tgccggcacg gatggcatca
    10861 gccggggagc gaaagacgtg cggtcgcccg tcgatcagga tttcgccctc atccggctgg
    10921 tacaggccat acagttgccg catgagcgtg ctcttgccgg cgccgttctc gccgagcaga
    10981 gcgtgcacct cgcccgcgct cgcgtcgaag gtcacgcctg cgttggcgat cacgccgggg
    11041 aaacgcttga cgattccgcg catctcgacg cgattgatcc gacgcgcagc gtgcgaaggt
    11101 gacagggaag cggaggtcat ggcccaacta tcgtcaattg ccacggagac acagaggcac
    11161 ggagaacatt cgatggatct gtgtctccgt ggctccgtgg cgatgtatgg agttcgccta
    11221 cggctcgacc ttgatcgtgc cgttcttgat gccctcgatg gcagcgtcgg cagccgcttt
    11281 gactgcaggc ggcaggtcgt actgcgggtt aaacgcgatc ttcaggccgt cgttcgccag
    11341 gcgcagcgtg aagacctcac cacccagtgt gcctgccttg tgcttctcga tcatctcctt
    11401 gatgaagggg gtccagtcat agacctggct ggcgacaacc agttcgggcg ccaacgaagc
    11461 ctgatcctgt tgcgtgccga accacagcac gccgcccgct tccttggccg cgccaatcga
    11521 gccgaccacc gattgcgacg agccggtgag cgcgtctgcg ccagccgcga tgtgggtctt
    11581 ggcggcttcg gccatgagcg ccacgtccga gaacgagccg gtccaggtct tggcgacctc
    11641 gacgtccgga tcggtggcct ccacaccctg cacgaacccg tcgatgtagg tcttggcgtc
    11701 gccgacttca atcgggccgg tcacaccgat tttctcgctt tcggtcagct cggccgccag
    11761 aacgccattc acgtagccgc cctcctcggc cgccgccgtg tacgcgaata cgttgggcag
    11821 gccgaaggtg ttgacgtcgg tgccccaggc gaagctggtg tcggggaagt ccggcgcgat
    11881 ctcctgaacc gacgagccat actgcgagcc gtgcccgatc accaggtcgt agcccttgct
    11941 ggcataatcg cgaagggcgg ccgccgcgtc gggcacgttg aacatgcctt ctgaataggc
    12001 gatctcgagc gcagacggac cacccatctc ggcctgcacg gcgagcaggg cagaatacat
    12061 cgactggctg aacgccatat cattcgtggc gctgggcatc accacggcga tgcggaacgg
    12121 ctgggctgcg gcgggcgcgg tcgtcacagc cccgccgccg caggccgaag ccagcagcgc
    12181 aaccaacagc agcagaatcc aaaagcgtcg cagggtcatt ctatcttctc ctcagaatgt
    12241 gagattcgaa agcgtagcgt cgcgatcggg tccgaatctg tggccggtca cctccttttc
    12301 tgtccgaatc agttcaagcc gtatcgttca gccttcaccg cgctcaaagg gcttgttcag
    12361 cgcagccggc tggcgcacgc gacttacggt cacagccaga acggcaatcg tcagtatgta
    12421 tggtagcatg acggccagct cgggcgcaat ctggaacccc agtacctgca tccacaactg
    12481 cagcgcgttg acggtactga agaggagcgc gccggccaat atgcccaatg gccgccatcc
    12541 gccaaagtac accagcgcca cggcgatgaa acccagcccg gcagtgagat tctcctgaaa
    12601 gagattgagc agcgcaagcg acagcgaagc gccggcgatc ccggccagga ttgcgcccag
    12661 gcatacgcag aagtagcgca ccgcgttcac gctcacaccc aacgagtcgg ccgccgccgg
    12721 attctgaccg actgacttga tcttgagtcc ccaggtcgtt ctgttcaaaa agagccaggc
    12781 gagtggcacc agcaggaaag cgccatacac cggcaggctc tgctggaaga cgatctcgcc
    12841 gacgatgggg atgtctccga gcaccggaat cttcaccgtc ggaaagccgt cgatggtctt
    12901 gaccgtgccg atcgcctcct tgtacagcag gctcgacagg cccaacccga agaggtataa
    12961 cccgatgccg ctgataccct gctcggcctt cagcgtgacg ctgatcacgg acatcaagag
    13021 acccatgagc agcccgacga ccgccgccgc agccatcccc agccacaggt cgccggtatt
    13081 cagcgcaacg tagaacccgg agaatgcgcc catgagcatg atgccgtcca cgccgaggtt
    13141 caggacgccg gaaagctgcg cgaacgtctc gccgatcgag gcgtacaaat acggcgtcgc
    13201 caggcggata gccgaagtga ggacgccgat gaagacgggc agcgtgaaga gatcactcat
    13261 gactaggctc caccccgctg ggcggggcgc aagcagcggt gctgtgcctc accggacccg
    13321 cgctggcgcc ttcggccggc agggtcccgt cttcggccgg catgtcctcc tccgccgcca
    13381 cctcgatggc gcgacgcgcg gcccagcggc gcgacagata ggctgacccg acgacgaaca
    13441 acacaatcag gccgagcagc gtgccgatca gcgccgaagg cacctgcatg gcacgctgca
    13501 tcttgtctgc gcccacgagc agcacgccga acaggaacga ggccggtatc gcgccgatcg
    13561 ggtgcaggcc gccgaacagc gctgccacaa tgccggtgaa gccgtagccg ctggtgatgc
    13621 cttgcaggag ccggtgctgc acgccgatga cttcgatcac accggccagc ccggcgaatc
    13681 cacccgccag cgtcagcgcc agcgcctggt gaaacggcac gcgaatgccg gcgtagcgtg
    13741 aagcgtccgg gttgagaccc accgcgcgga tacgatagcc gatcgtcgtg cgccacaaga
    13801 agacgtagac cagcacggcc aggatcacgg ccaggatcgc gccggtgtgg agcaaagtct
    13861 gcggcaccag gcgcggcagc cacacgctgt ccggcagccg ctccgactgg gccagatacg
    13921 tgcccgccgc gacgccggct ggatcgatca tcgggccgcg caacaggtag ttcatgagtt
    13981 gcagcgcgat ggcgttcagc atcacggtgc tgaggatctc gttcacgttc aggcgggcct
    14041 tgaggatacc ggggatgaag ccccagaatg ccccggccag gaagcccacg cccatcgtcg
    14101 tcgggatcaa cagccaggat ggccacattc gaaatgtcaa cgaaaaccaa gttcccgcca
    14161 gtgcgcccag aatgatctgg ccctcgccgc cgatgttgat cacgctggcg cggaaggcga
    14221 tcacgatgcc gagcgcgacg aggagcagtg gcgtggcctt gaccaccgac tgagtgaggc
    14281 ccgagacgtc acccagcgcg ccgccgatca tcgccgcgta cgcttcaatc gggttgacgt
    14341 tcagcagcag caggagaatc gcgccgacga gcatcgccgc gagcacggcg aggatcgggg
    14401 tgagcgcgcg gagcgccacg tcgcgccagt cgaaacgagg tgcacggatg cgacggggag
    14461 ccgaggcgac cctatcgagc ataggctcgt tcaatcgaac catcccgggg ccggctttcg
    14521 aaatccgctg cttgcgcccc gcccagcggg gtcgattcgc cggcaagctg cttgcgcccc
    14581 gcccagcggg gtcgctgtct tactcctgct caaaatgcgt ccccagcgcg gacggcggtt
    14641 cgctgcgcaa gacgccgcgc acggtaggcc agcgatccag caatcgtccg atcgagtcgc
    14701 tggacaccag aagcaaggtg aagatcatca gcgggaaggg cagaatcgac agcacctgtg
    14761 acaggttcgg caacaccggc tgcagtttca aggccagaac ctgcaaagcg ccgaacagat
    14821 acgcgccgaa tgccacgcga acaggatgcc acccgccgaa gatcacgatc gccagtgcga
    14881 tccagccgag gccggccgta tgccgatagg accagcccag tttcacgtcg agcgagtacg
    14941 ccgcgccggc gatgccgacc agcgcccccc caacggccgc gtacacataa cgcatccggt
    15001 tgacggggac gccgcgcgca tgcgccgcct cgggccgctc gcccatgccc tgcaattcca
    15061 gcccgcgccg cgtcctgaac acgaacaggt aggccgcaat aatcgtcagg atgctggcgt
    15121 acacgaccag gtcctggtca aacaagattg gccccaggaa cggaatgtcc tcgaggatcg
    15181 gaatgggcat gtgcggcacc gacgggcctt gaatccgcac gaagggattg cccaagaacg
    15241 agctgacgtc ggcgcacagc agcgtcagga cgaaacctac cgcgacctgg ttgagccgca
    15301 gtgaaatgct ggcgtacgcg acgagcagcg cgacgagcag cgacacgatc gccgccgcca
    15361 cgaagcccag ccacacgtta cccgtgaggt aggccgccgc gaagccgatc atcgccgaga
    15421 ggagaatgct cccgtccagc gacaggttga tcacgccgga tttctcggtg atagcctcgc
    15481 caatcgtcgc gaagatcagc ggcgccgagg ccaacaggat cgaagcgagg gcggcttcca
    15541 tgccaactcc tagaaggata gttatcagtc atcagtcatc agtgatcaga agccagtggt
    15601 cagcaagcac tggctgctga gcactgaata acgaacattg accactgacc accgaccact
    15661 gatcactgat tactgatcac tctctttgct tgccagccgc gggccagcag cacaaacaga
    15721 actaacatac cctgcagcac accacccagc gacgagtcca ggttgagccg caattgcaac
    15781 tgcgtgctcc cgacggtgat catcgcaaag aatagcgcaa tcggcgcgat ccaggccgcg
    15841 cggaagcttg ccagcagcgc gacaagaatg ccgaggtagc cgtagccgcc tgagacggcg
    15901 ggcacgagct tgtgatgaaa gcccgtagcc tgaatcgcgc cagccagccc ggcgaatccg
    15961 ccgcacagcg cgaaggcgat caacatgtag cggtgggtgg gtatgcccag cagaaacgcg
    16021 ctgcgcatgt tcttgcccac ggccttcagt tgcaggccga acagcgtgcc gcgcagcagc
    16081 acgtatacga cgatcacagc ggcgatcgcc aggacgatcg ccagcggcgc cagccgcaga
    16141 ccttcgagcg tgggcagcca tgcctcgcgc ggaaacgtat cggtaccgct cgtcgaggca
    16201 atacctgcac gcttccaagg cccgatgatc agatagatcg tcaagccgat ggcgacgaaa
    16261 tttaagccca gcccgccgaa gatttcattg accttgccaa aggtgcgcag cacgccgacc
    16321 agaagtcccc acaccacgcc gccggcgatc ccgccgatca tcgtgagtgg cacgagcacc
    16381 tcggccgggg ttttcacttc ccgcgcgacc cacgctgcgg cgatcgcgcc gaagacgatc
    16441 tgcccctcaa cgccgatgtt ccacaaccct gcggcgaacg taatcagcag gccagatgcg
    16501 cacaagacca gcggcaccca agccatcagc gtgtccgaaa tcttctccgg cgtcccaaac
    16561 gcaccctcga tcaggtgttg gaacgcctgc aggggtgccg cgccgacgag caacaggagc
    16621 accaaggcga gcgccacggc cagaacgacc ggaagcagac ccagccccac ccgtctcaat
    16681 gtgatcatgc gttaactccg aacgtaaagc gtgaagcgtg ggcgtttcat ccttcgactt
    16741 cgctcaggac aggcgtaccg cgcatcacgc gacttgcttg cctccaatca gatgcccgag
    16801 ctgctccacg tctgtctccg acgcgttcag cacattcaac acgcgcccgg cgaagaaaac
    16861 catgatccgg tcgctgtact gcagcagctc gtccagatcg gcggatgcaa acacaatggc
    16921 cgtgccatgt gcccgccggg ccagcaactg catccacacc cagttcgcag attcgacgtc
    16981 cagtccgcgc gtggggtgtt ccatcaatag cagccttaac ttcggcggga gcatcgccaa
    17041 cagtagacgc tgctggttgc cgcccgacag cgcctcagcc gtggaagtgg gtcgccccat
    17101 aatttcgaag aattggatgg tctcttcggc acgctgacgc gcgcctgccc aatcgacgaa
    17161 aaagcgccgg tcaccactgg ccagaacgaa gtgttccgcc agcgtcatcc cggcgaccag
    17221 cccttcttcg agacggccag ccggcaaata gtggacgccg gccgataaga actcgcggta
    17281 agaatgaccg gtcaggtcgc gcccgcccac gtgcacgcgc ccggcagacg gccggagcaa
    17341 accggcgcag gcgcgcagcg tggtgcgctg gccgctgccc tccagcccgg ccaggccaat
    17401 cacctcaccg gcgttgacac tcagcgacag atcgttgatc gacagcaggc gatcgcgcag
    17461 gctgacgtga tcgagctgca gcactggctc accctgtttg accggcaacc gcccgcctgg
    17521 cacgacgacc tgtccgaaca tcatctcgac cagcctctcg accgggcatg gcaggcccgc
    17581 ctcgccgacg accttgcctc gccgcatcac ggtgaccttg ccgcacagct cctcgacctc
    17641 ttccagcttg tgcgacacga agatcacgct catgccctgt gccgccagcg cccgcagcgt
    17701 ctcgaacagc ttgacgcgct gtggagccga gatgcccgtc gtcggctcgt cgaggatcag
    17761 cacgcgtgcg cccagccaaa gcagccgcac aatctcgagc tgctgccgct cgcccaccgt
    17821 caacgctcgg gcgggcatgt cgggatcgag cacaaagccg aactggtcgc acagccgctt
    17881 caacccgcgc tcggccgagg cgcgatcgag gcgtagcggc cccggactgc cgagcaggaa
    17941 attgtcgatc acgttgaacg gtaagaagac caacgggtcc tgatgcagca tgccgatacc
    18001 gtgacggatg gcatcgacgg gcgagcggat gcgggctggc ttgccgtcga ggacgatctc
    18061 gccggagtcg gcgggctgga acccggacag aattttcatc agcgtggatt tgcctgcgcc
    18121 gttctcgccg agcaagccgt gcagcgtgcc ggcctcgacg gtcagcgtga cgccgtcatt
    18181 ggcgcgcacc ggcccgaagt gtttgtggat gtctcgcagt tcgacgcgca tgtggctaat
    18241 cgcttatagc gcatggctta tcgcggatgg ccggatgcac tgccgccggc catccgcgat
    18301 tcgcaataga cgatacgcta tatgcgatac gcgatatgct cgctactggc tcgtgctcgc
    18361 gccgagcatg ccctcgagca actgcggcat gtaccagacc tgcaggtcgg ttgcgacctc
    18421 gccgtcctcc agatacgtcg aaccgtcctg gtagttcagc ggacctttga acagaacgat
    18481 cgacccatcg gccaggccag agatgaactc gtcgagggtc gcgctattgt cttccgacag
    18541 cgccgggcct ttgacaaagc cgaccgcggt ggtgtccgga ttgttgatgt cggtccagtc
    18601 cgggccgttc cactcgaatg tcgcttcaaa cgatccatcg atgacggcct gcgcgacccg
    18661 cagataggca gggccccagt tgaagtacgg cacgcccagg cagataaacg gcgcttcctc
    18721 gcacgcgcct tcgaagtcgt agggcacggc ccacacggtt tcgtcggccg ccgcgcgctg
    18781 cccggcgacc accagccctt cggtggtgtc gatgccggag atcaccacgt cggcgccgcc
    18841 gtcaaagaat tcgttggcga cctgcgtcgg gtcgagcgtg acgccgggga tgttgaacca
    18901 gaagccgatc catgtcacct cgaacttcag atcggcgggg tcattgccgc gatagttctc
    18961 ccagcagtac tt
//
LOCUS       DBNHXB010000279        12639 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_73101, whole genome shotgun sequence.
ACCESSION   DBNHXB010000279 DBNHXB010000000
VERSION     DBNHXB010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 12639)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12639)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12639
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_73101"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="AC162G_11305"
     CDS             <1..326
                     /locus_tag="AC162G_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR5076451.1"
                     /translation="AEARGDGLGPGRGIGDVNGDGHADLIIAAYTSDAGAVDGGRAYV
                     RSGKDGSLMRTITGRIANDNLGVDALAVGDVNDDGLVDYLVTAVGNSFAGLDTGRAYI
                     VAGVP"
     gene            complement(371..1369)
                     /locus_tag="AC162G_11310"
     CDS             complement(371..1369)
                     /locus_tag="AC162G_11310"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR5076452.1"
                     /translation="MKDTIRKLVEAYGPSGFEDQIRDIIRKEIGGLADSVRVSSLGSL
                     IAVKKGTGGKNAKRVMLAAHMDEIGLMVTHIDEKGYARVTHIGGIRPLNCIGQRVQFA
                     DGTIGVVLAERLEDPSKAPGMEHLFIDTGARDRASSKVKVGDAAGFVQPFVDMGQRIA
                     AKTMDDRIGCAVQIEVMRRLARTPHEVHFVFSVQEEIGLIGARTAGYEINPDVAIAID
                     VTLTGDTPKALPMAVELGKGPAIKVQDGMMIAHAGVKNLMIRRAEESKTPYQLEILRA
                     GSTDAAAIQLAREGVPSGCVSIPCRYVHSQSEMVDTTDVLQCVNLLLAILRKPIEV"
     gene            complement(1494..2543)
                     /locus_tag="AC162G_11315"
     CDS             complement(1494..2543)
                     /locus_tag="AC162G_11315"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR5076453.1"
                     /translation="MTITSYLRELSEAAGVPGDERAVRKMIEDAVRDHVDEMRVDTLG
                     NLLALKRARRDKGRPMRVMIDAHMDEVGFMIVGHTGDGGLKFRAVGSLDPRILPGKVV
                     SVGPDRIPGVIGVKPMHLARDTSAVQQIDALVIDIGASSKEQAEDAAKVGTYAAFRTL
                     YRSLGGGKVLGKAFDDRAGCAVLIDILQGPRLPVDVLAAFTVQEEVGLRGARAAAYAL
                     NPDAGFAIDVTPANDLPQPEGEDRSPNARLDAGPAIYVMTRGDVSDPRLVKHALEVGE
                     KRGIPHQIRQPGAGGTDAGAINRSRAGVPAIAISTPCRYIHSPVCVLSLADLRNCADL
                     VRESVAQMTPRVLRR"
     gene            complement(2669..3718)
                     /locus_tag="AC162G_11320"
     CDS             complement(2669..3718)
                     /locus_tag="AC162G_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5076454.1"
                     /translation="MNLTQLIKKLSETTGISGHEAAIRQVIGEELGRCADEVHVDKMG
                     NLVALKRGTGRRPRRRILLAAHMDEIGLMVGGIDKGFLRVERVGGVDPRVMLAQEVVV
                     HGRRDLPGVIASLPPHLQADADKDKTVPVDKLWVDAGLSEAQARRLVRVGDLVSIRQT
                     VVELKNDLLAGKAFDNRVSVAAIAACLEGLVEHQHGWDVMAVATVQEETTMLGAATSA
                     FRHAPDVAIAIDVTFGAQNGSNDVETYSLGKGPTIMIGPNAHPQIADRLRDVARRIEM
                     SVQVEPLPGSSGTDAWAIQVAREGVPCGIVGIPLRSMHTPVETVSLKDVERAGRLLAA
                     FIADLDEAFYKSLTD"
     gene            complement(3837..4439)
                     /locus_tag="AC162G_11325"
     CDS             complement(3837..4439)
                     /locus_tag="AC162G_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076455.1"
                     /translation="MLSDVKHPTAADTSARRRDELAGRRARKPAEALPDLDQHHHRRR
                     NLLLRWDSRLTVRSLKSDDHGHIHADGHAVPDRARRDSIADPYAASVADAVQHADLHA
                     HVDPDGHADAKPDEYRNTDTDGHAGLSDADGNWNGGADSDANADIDLHAHTDTGDHRH
                     FHANAYRHGDAHADRDAYADRDAHADRDAYAIAQEHMAAF"
     gene            4812..7166
                     /locus_tag="AC162G_11330"
     CDS             4812..7166
                     /locus_tag="AC162G_11330"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase TgpA family protein"
                     /protein_id="HIR5076456.1"
                     /translation="MKHEMMPSKPRLDFGILLLLAFTLAMAAFTVASSDWAPGLDRLQ
                     PTSLAALLLGTLLARSRFRPLSAHLLASAYGVAWIAFASLAYLPAAEEAATLGESLIA
                     FGRHLGEWIASLLQTGVGKDNFMFLLSMMAIFWLLGYLAAWYTFHTVRVLRVALLPGL
                     VILVNLYYYGGRMQLSIFLYLYLAGMLLYLARVHYMLREREWRQARIGFDSDVRSSFV
                     RGSSIVALCAMIVAWAVPEFAPMPQFDELWRQVSRPIRSIEDGFTRVFSALEGEGPPL
                     TNPFGRTLGFAGPRALGDTVLMDVYVHPSPDNAHALARYWRAGTYDVYTSNGWLSSAA
                     DSYVYRPGADPLAVPYAQRSNVRQTVTFYFQRTSLLISAAQPVYFNRDAEADAMLAAM
                     PAGSIQGGSQLVVEPVFVTSRDMLRAGDNYDVVSSLSTADVDTLRGAGTDYPDWIVER
                     YLQLPEDFSPRVKDLARQIVTEAEATNPFDQAAALEQWLRRNIGYNEKIEGPQLGQDG
                     VDYVLFESRAGYCDYYAAAMATMARALGIPARVAVGYARGEYEPSGEVYRVRERDAHA
                     WVEVYFPGYGWVEFEPTAAQPVINRPVPAKEGDLPEPALPPTPSARDLERLSRLGEDE
                     GLDVFDSSGATSAAQRWLPGAILFVASALIVCVAIAGVAIYVVENRGLRSLRGARWAY
                     ARLVRLAKWLRVDLTAYQTPYEQAELLGEAAPSNRTAIQEITVDFVRETFGRDESGVD
                     RVRSIWRQVHLGLWWAGLKRRIFSATRPYRPKDFLQLRFPRRRV"
     gene            7383..9137
                     /locus_tag="AC162G_11335"
     CDS             7383..9137
                     /locus_tag="AC162G_11335"
                     /inference="COORDINATES: protein motif:HMM:NF023277.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5076457.1"
                     /translation="MSDTSSFGPPSDITLILKTPAAQADLKSMLEALTGALERQASAL
                     TKMPASPDVLQAQKSLLEMQRILPKFRQMVDTLAREREALRALYEVGQAVNSTLDLHD
                     VLNQVMDHIIELTGAERGFLMLRDERTGQLEFRVARNMERETINSSSFEVSRTIVHQV
                     ATGGVAVVTTNAQADPRFATQESIVAYSLRSILCVPLRARDRITGVIYADNRIKTGLF
                     SDRDRDLLAAFANQAALAIENARLFESVRRNLAAITEMKNLMDNVFASIASGVVTTDV
                     IDRVTLFNRAAEAIFGVPARDIVNRPYTSALSAAHDLAPLIDEVKANETVVAREMMPT
                     LPERGTVSWRLNVSPLKDAAHTTQGVAIVIDDLTEQRFIKETFQRYVGPRVVQRLLSD
                     PGMLKLGGARRVITTYYADIRGFSTFGENLKPEKLVEILNAYLGLAADAVLGEEGTLD
                     KFMGDAVMGFFNAPLDQADHTLRAVRAAIATQKAIQEYRERVPSGERLSYGVGINVGE
                     AVVGNIGNAQQQNYTAIGDSVNYARRLQENARHDQILISQAVYDLVKDRVRVNALEPL
                     LVKGHSKPEPVFEVLGLK"
     gene            9241..10737
                     /locus_tag="AC162G_11340"
     CDS             9241..10737
                     /locus_tag="AC162G_11340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5076458.1"
                     /translation="MIVATVLYAIATTALGLYGVYVLALVAQCLRHRRAPRLPLPPVP
                     RDEYDLPVVTVQLPLYNEPAVAERIVDAAAALDWPRHRLQIQVLDDSTDDTTAMARAR
                     AESHRRRGIDVMLIHRSSRDGYKAGALANGLKSARGEYVAVFDADFVPAPDFLRRLMP
                     YFSASASIGFVQARWEHLNYHRSALARALAVAVDGHFVVEQFARNRSGWPTIFNGSAG
                     IWRRSCIELSGGWHTDTLCEDMDLSYRAMFAGWRGVFVPDVSVPQEEPESIGAVKLQH
                     ARWAKGGAQCLRKHALSILRASSWSPIQKAAGFAYLAGYTAHLLMVLVVLLWLPLAMG
                     GPSLQQGPLAFLGLSGLGLPFAHGVAQWALHRGDGSWLRRLSYLPLLLVVGFGIAFSN
                     ALAVLSGLIRQGGEFHRTPKGAADTTRLADSRPRAELLAWLEIGMALYAIAAGLVLWI
                     AGHPTTSAFVATFALGFGSVGIGSLVEIARVRGNAPARGAQRRRRAAL"
     gene            complement(10896..11357)
                     /locus_tag="AC162G_11345"
     CDS             complement(10896..11357)
                     /locus_tag="AC162G_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR5076459.1"
                     /translation="MFSREKPQQRPAAAPPSAPAGGAARTIETVIGSTAHVKGSITSD
                     AGIRIDGIFEGEIRVAGNLIIGETGKVIADISADNVSVSGAAKGTIEAAGRLEILSTG
                     RVWGDITVASFLIDEGGFFRGQSIMPGEPDMPMLEPPGGAAVTPGEVIDAQ"
     gene            complement(11443..>12639)
                     /locus_tag="AC162G_11350"
     CDS             complement(11443..>12639)
                     /locus_tag="AC162G_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076460.1"
                     /translation="AFLLAPWEALTPLWVEQYANNHPLDPRDVTLVYVAAGSFNPWLD
                     NVFAYFDRGPVYLADYRRQVVEGGLFRLRPDGESPPLWRVAALGDASLPALSTTLDVI
                     AEGEIELLGVSLDRTALQAGDTAHITLAMRAPVTPTHILMPYADVGGRAYRWTTDSRV
                     LSTDWRPGEVIVERYEIALPFSTPPGEYPLSLGVADLTEGRDLRLRKSVGISEVSMVP
                     VGALRVEVPQLAPAALSSPAWADFGAQIALVGASASGNGQVVHAASRAWTEPLVVRPG
                     QSIQVWLDWLGQRQPLDNYKVFVHLITPGEQLAAPPADFYTPLGGAFPTHLWIPRWIE
                     GQGVSDPYTLTLPPDVPPGDYLLQAGLYGLTSNRRVYILGADGNLAGDRVILGPVTVA
                     GQSSSP"
BASE COUNT         2099 a         4017 c         4083 g         2440 t
ORIGIN      
        1 acgccgaagc aaggggagat ggcctgggac ctggacgagg aatcggcgat gtgaatgggg
       61 atggtcacgc cgacttgatc attgcagcgt acacatcgga cgcgggcgcc gtagatggtg
      121 gcagagctta cgtccgttca ggcaaagacg gctccctgat gcgcacgatc accgggagaa
      181 ttgcaaatga caacctgggc gtggatgctc tggccgtagg cgatgtcaac gacgacggcc
      241 tagtagacta tcttgtcacg gcggtgggca acagcttcgc ggggcttgat accgggcggg
      301 cctacatcgt ggccggagtc ccatagagta aagaaaccgg gtttctgcaa gaaacccggt
      361 ttcttgcctg ctacacctcg atcggcttgc gcaggatggc tagcagcagg ttcacgcatt
      421 gcagcacgtc ggtcgtatcg accatctccg actgcgagtg gacgtagcga catgggatcg
      481 acacgcagcc gctgggtacg ccctcgcgcg cgagctggat cgcggctgcg tcggtcgagc
      541 cggcgcgcag gatctccaac tggtacggcg ttttgctctc ttcggcgcgc cggatcatca
      601 agttcttcac accagcgtgg gcgatcatca tcccgtcctg cactttgatc gccgggcctt
      661 tgcccagctc gacggccatc ggcagtgcct tcggcgtgtc gccggtgagc gtcacgtcga
      721 tcgcgatcgc gacgtccggg ttaatctcgt agcccgccgt gcgcgcgccg atcaggccga
      781 tctcctcctg cacgctgaac acgaagtgca cctcgtgcgg cgtgcgggcc aggcgccgca
      841 tcacctcgat ttgcaccgca cagccgatgc ggtcgtccat cgtcttggcg gctatgcgct
      901 gtcccatgtc gacgaacggc tgcacgaacc cggccgcgtc gcccaccttc accttcgacg
      961 atgccctgtc gcgtgcgccg gtgtcgataa ataaatgctc catgcccggc gctttgctcg
     1021 ggtcttccag ccgctcggcc agcaccacgc cgatcgtgcc gtccgcgaat tgcacgcgct
     1081 gcccgataca gttgagcggc cggatgccgc cgatgtgggt gacgcgcgcg tagcccttct
     1141 cgtcgatgtg tgtcaccatc aggccgattt cgtccatgtg cgccgcgagc atcacgcgct
     1201 tcgcattctt gccgcccgtc cccttcttga cggcaatcag gctgcccagt gagctgacgc
     1261 gcaccgagtc ggccaggccg ccgatttcct ttcggatgat atcgcggatc tggtcttcga
     1321 acccggacgg gccgtaagcc tcaacgagtt tcctgatcgt gtctttcaac tctgcactcc
     1381 cctagatctc tcagagcgtg tccgaagtca accaccaaga caccaaggca caaaggatca
     1441 cctggcattt cttcgtgact ttgtgccttt gtggtgaatt cttcggcagc ctctcagcgc
     1501 ctgaggacac gcggcgtcat ctgcgctacg ctctcgcgca ccagatcggc acagtttcgc
     1561 aagtcagcca ggctcaagac acacaccggc gagtgaatgt agcggcaggg cgtcgagatc
     1621 gctatggccg gcacgccggc gcgggatcgg ttgatggccc cggcgtccgt ccctcccgcg
     1681 cccggctggc gaatctgatg tggaatgcct cgcttctcac cgacttccaa ggcgtgcttg
     1741 accaaacgcg gatcggacac gtcgccgcgc gtcatgacgt agatcgccgg gccggcgtcg
     1801 aggcgcgcgt tcgggctgcg atcttcgcct tccggctgcg gcaggtcgtt ggcgggcgta
     1861 acgtcgatcg cgaaacccgc gtcggggttc agcgcatagg ccgccgcgcg cgccccgcgc
     1921 agcccaactt cttcctgcac ggtgaaggcg gccagcacgt cgaccggcag gcgcggtcct
     1981 tgcagaatgt cgatcagcac ggcgcacccg gcgcggtcgt cgaaggcttt gccgagcacc
     2041 ttccccccgc cgagggatcg atacagcgtg cggaacgccg cgtacgtgcc gaccttcgcc
     2101 gcgtcctcgg cctgctcctt gctgctcgcg ccaatgtcga tgaccagcgc atcgatctgt
     2161 tgcacggcgc tcgtgtctct ggcaagatgc atgggcttca cgccgatcac gccgggaatg
     2221 cggtcgggcc cgaccgacac gaccttaccg ggcaagatgc gcggatcgag tgagcctacc
     2281 gcgcggaact tcagcccgcc gtcccccgtg tgcccgacaa tcatgaaccc gacttcgtcc
     2341 atgtgcgcgt cgatcatcac gcgcatcggg cggcccttgt cccgacgcgc tcttttgagc
     2401 gcaaggaggt tgcccagcgt atccacgcgc atctcgtcga cgtgatcgcg gacggcatcc
     2461 tcgatcatct tgcgcacggc gcgctcgtcg ccgggcacgc cggctgcttc ggagagttct
     2521 cgaagataag aggtgatggt catggcctgg tattgtacga tctgggaccg gttatcagta
     2581 atcagtgatc agtaatcagt gatcagttat tggtaattgg taatccgtgg tcactgctca
     2641 ccaatctcta attactaatc tccaattact aatccgtcag cgacttgtag aacgcctcgt
     2701 ccaggtcggc gatgaacgcg gcgagcagtc gacccgcacg ctcgacgtct ttcagcgaca
     2761 cggtctcgac cggcgtatgc atcgagcgca gcgggatgcc gacgatgccg cacgggacgc
     2821 cctcgcgcgc gacctgtatc gcccaggcat cggtgccgct cgatcccggt aacggctcga
     2881 cctggacgct catctcgatg cgtctggcga cgtcgcgcag ccggtcggcg atctgaggat
     2941 gcgcgttcgg gccgatcatg atcgtcgggc ctttgccgag cgaatacgtc tcgacgtcgt
     3001 tcgatccgtt ctgcgcgccg aacgtcacgt ctatggcaat ggcaacgtca ggggcgtgcc
     3061 tgaacgcgct cgtcgccgcg ccgagcatcg tcgtctcttc ctgcaccgtc gccacggcca
     3121 tcacatccca gccatgctga tgctcgacca gcccttcgag acatgccgct atggccgcca
     3181 ccgatacgcg attgtcgaac gccttgcctg cgagcaggtc gttcttcagc tcgacaaccg
     3241 tctgccgaat cgacaccaga tcgccgacgc gcaccagccg ccgggcttgc gcttcactca
     3301 aacctgcgtc gacccacagc ttgtcgacgg gaacggtctt gtctttgtcg gcgtcagctt
     3361 gcaggtgcgg cggcagcgag gcgatcaccc ccggcaggtc gcgccggccg tggacgacga
     3421 cctcctgtgc gagcatcacg cgcggatcga cgccgcccac gcgctcgacg cgaaggaagc
     3481 ccttgtcgat gccgccgacc atcaggccga tctcgtccat atgcgcggcg agcaggatgc
     3541 gccggcgggg acgccggcct gttccgcgct tcagcgccac tagattcccc atcttgtcga
     3601 cgtgcacctc gtcggcgcag cggccgagtt cctcgccgat cacttgtcgg atggcagcct
     3661 cgtggccgga gatgccggtt gtctcagaca gtttcttgat gagttgagtc aggttcatcg
     3721 agttccgggt cggatggtgt ttcgaacgcg caggagcgac aggcatcctt tgaccctacg
     3781 cctgcgctca gagcttgccc tgagcctgcg aaggggcagg cctcactgcg tttcgctcag
     3841 aatgcggcca tgtgctcttg tgctatggcg taggcgtctc ggtcggcgtg ggcgtctcgg
     3901 tcggcgtagg cgtctcggtc ggcgtgggcg tctccgtgtc ggtaggcgtt ggcgtgaaag
     3961 tgtctgtgat ctccggtgtc ggtgtgagcg tgaaggtcga tgtcggcgtt ggcgtcgctg
     4021 tcggcgcctc cgttccagtt gccgtcggcg tcggagagac ctgcgtggcc gtcggtgtcg
     4081 gtgttgcggt actcgtcggg cttggcgtcg gcgtggccgt cggggtcgac gtgggcgtga
     4141 aggtcggcgt gttgaactgc gtcggcgacg gaggcagcgt acgggtcggc aatggagtcg
     4201 cgcctggcgc ggtcggggac ggcgtggccg tcggcgtgaa tgtggccatg atcgtctgat
     4261 ttgagggagc gaacagtaag gcggctatcc cagcgcaata gcagattccg gcggcgatga
     4321 tgatgctgat caagatcagg tagcgcctcc gccggctttc tggcacgtct gccagccagt
     4381 tcatcgcgtc tcctcgcgct cgtatcggcc gctgtcggat gcttcacatc gctcagcatg
     4441 accaaacgcc gatctactta gcccggctgt actggtgttg gtctcgccgc agaggccgac
     4501 ggcacacgga ggccgcggcg attcctgagc tgcgcggcct ctctgcgcgt cgttggcgtc
     4561 tgcgatgagg agcaggccaa tcggctcatc aacacggaag agggagactg cctgggagcc
     4621 gaagcgcgcg aatcataaca tcgttgatcg gggaagtcaa cttgcctttc agtcgccgtg
     4681 taccctgtgt ctttgctgtt gacctcggct cacagcacgg ttatacttct ttgtgtgttc
     4741 tgaacctctt gggtgggata tgcgtatctt agggggtatg tgacgatctg gtcacgggac
     4801 aattgcacga gatgaaacac gaaatgatgc catccaagcc gaggctcgac ttcggaatct
     4861 tgcttcttct ggcgttcaca ctggccatgg ccgcgtttac cgtggcgtcg tcggactggg
     4921 cgccggggct ggaccggctc cagccgacat cgctcgcagc cttgctgctc ggcacactgc
     4981 tcgcgcgcag ccggttccgc ccgctgtcgg cgcatcttct ggcgagcgcg tatggcgtgg
     5041 cgtggatcgc gttcgcctcg ctggcctatc tgccggctgc ggaggaggct gcgaccctcg
     5101 gcgagagcct gatcgctttc gggcgccacc tcggagaatg gatcgcttcg ctcttgcaga
     5161 ccggcgtcgg caaggacaac tttatgttct tgctgtcgat gatggcgatt ttctggctgc
     5221 tcggatatct tgccgcgtgg tataccttcc acaccgtgcg cgtactccgc gtcgcgctgc
     5281 tgcccggcct ggtgatcttg gttaacctgt actattacgg cggccggatg cagttatcca
     5341 tttttctgta cctgtacctc gccggcatgc tgctctatct ggcgcgcgta cactacatgt
     5401 tgcgcgagcg cgaatggcgg caggctcgga ttggattcga tagcgacgtg cgcagctctt
     5461 tcgtgcgcgg cagctccatc gtcgccttgt gcgcgatgat cgtagcctgg gccgtgcccg
     5521 aattcgcgcc catgccacag ttcgacgagc tgtggcgtca ggttagccgg cctattcgat
     5581 cgatcgaaga tggcttcacg cgcgtgttct ctgccttgga gggcgaaggt ccgcccctga
     5641 cgaatccgtt cgggcgcacg ctgggctttg ccgggccgcg cgccctgggc gacacggtgc
     5701 tgatggacgt gtacgtccac ccgtcgcccg ataacgcgca tgccttagct cgatattggc
     5761 gcgccggcac gtacgacgtg tacacgtcga acgggtggct cagctcggct gcggattcgt
     5821 acgtgtaccg tccgggcgcg gacccgctcg ccgtcccgta tgcccagcgc tcgaacgtga
     5881 ggcagaccgt cactttctat ttccagcgca cgtctctatt gatctctgcg gctcaacccg
     5941 tatatttcaa ccgagacgcg gaggcagatg cgatgctggc cgccatgcct gctggcagca
     6001 tccagggcgg ctcacagctc gtcgtcgagc cggtgtttgt gacgagtcgc gatatgcttc
     6061 gcgcgggcga caattacgac gtcgtctcgt ccttgtcgac ggccgacgtg gatacgctgc
     6121 gcggggcggg aacggactat ccagactgga tcgtcgagcg ctatctccag ttgcccgaag
     6181 acttttcgcc gcgtgtgaag gaccttgccc ggcagatcgt gacagaagcc gaggcgacta
     6241 atccattcga tcaggcagcg gcgctcgagc aatggctgcg acgtaacatc ggctacaacg
     6301 agaagatcga agggccgcag ttaggacaag acggtgtcga ctacgtgctg ttcgagtcac
     6361 gcgccggtta ctgtgactac tacgccgctg caatggcgac gatggcgcgt gcgctgggga
     6421 tccctgcgcg ggtcgccgtg ggctacgcgc gcggcgagta cgagccatct ggcgaggtct
     6481 accgcgtgcg cgagcgcgac gcccatgcct gggtcgaggt ctacttcccc ggctacggct
     6541 gggtcgagtt cgagccgacg gcagcgcagc ctgttatcaa tcggcctgtg ccggccaaag
     6601 agggggattt gcctgagcct gcgctgccgc ctacgccgtc ggcgcgcgac ctcgagcggc
     6661 tctcgcggct cggcgaggac gaaggcctcg acgtgttcga ttcgagcggg gcgacaagcg
     6721 cggcccagag atggctgccc ggagctatcc tcttcgtcgc cagcgcgctg atcgtgtgcg
     6781 tggcgatagc cggcgtagcg atttacgtcg tcgaaaaccg cggcctgcgc agcctgcgtg
     6841 gcgcgcgctg ggcttatgcc cggctcgtgc gcctggcgaa atggctgcgc gtcgatctca
     6901 cggcgtatca aacgccgtac gagcaggccg agctgctcgg cgaggcggct cccagcaacc
     6961 ggaccgcaat ccaggaaatc accgttgact tcgtgcggga aactttcggg cgcgatgaaa
     7021 gcggtgtgga tcgggtgcgt tcgatctggc gccaggttca tctcgggttg tggtgggccg
     7081 gccttaaacg ccgcatcttc agcgcgactc ggccttatcg gcctaaggac ttcctacagc
     7141 tacgtttccc caggcgacgc gtgtagagca cgcggtttaa ccacggattc cacggatgtc
     7201 acaaatggcg gcccctcaat atgtccgtgt catctgtggt aattcttttg gttgcgacac
     7261 gcttcgcagg tctgccctat gcctcagcgg cagagaaaac gccgcttcgg tttgtcacct
     7321 ccaacgagtt gtactataat catcgtgctt cgagccaagt ggccgtctcg tggagacctg
     7381 aggtgtccga cacatcctcg tttggaccgc catccgatat cacgctcatc ctgaagacgc
     7441 cggccgcgca ggccgatctg aagtcgatgc tggaagcgtt gacgggtgcg ctggagcgtc
     7501 aggcttcggc cctcacgaag atgcccgcca gccccgatgt gctgcaggcg cagaagtcgc
     7561 tgctggaaat gcagcgcatc ttgcctaagt tccggcagat ggtcgacacg ctggcccgcg
     7621 agcgtgaagc gctccgtgcg ctgtacgagg tcggccaggc cgtcaactcc accctcgatc
     7681 tccacgacgt gctcaaccag gtcatggatc atatcatcga gctgacgggg gccgagcgcg
     7741 gcttcctgat gctgcgcgac gaacgcaccg gccagttgga gttccgagtc gctcgcaata
     7801 tggagcgcga gacgatcaac agctcgtcgt tcgaggtgag ccgcaccatc gtccatcaag
     7861 ttgcgacggg gggcgtcgcc gtcgtcacca cgaacgccca ggccgatccg cgatttgcca
     7921 ctcaggagag catcgtcgcc tacagcctgc gcagtatcct gtgcgtgccc ctgcgggcgc
     7981 gtgatcgcat caccggcgta atctacgccg acaaccgcat caagaccggc ttgttctccg
     8041 accgcgatcg cgatctgctg gccgcgtttg ccaatcaggc tgcgctggcg atcgaaaacg
     8101 cgcgcctctt cgagagcgtc cggcgcaacc tggcggccat caccgagatg aagaacctga
     8161 tggacaacgt attcgcctcg atcgcgtcgg gcgtggtcac caccgacgtg atcgatcgtg
     8221 tgacgctctt caaccgcgcc gccgaggcca ttttcggcgt gcctgcccgc gacatcgtca
     8281 accggcctta cacgtcggcg ctgagcgcgg ctcacgacct ggctcccctg atcgacgagg
     8341 tcaaggcaaa cgagacggtc gtcgcgaggg agatgatgcc gacgctgccc gagcgcggca
     8401 ccgtctcgtg gcgtttgaac gtctccccac tgaaggatgc cgcgcacacg acgcagggag
     8461 tcgccatcgt catcgacgac ctgaccgagc agcgcttcat caaggagacg ttccagcggt
     8521 acgtcgggcc gcgcgtcgtg cagcgactgc tgtctgatcc tggcatgctg aagctgggag
     8581 gcgcgcgccg cgtcatcacg acgtactacg ccgacatccg cggcttcagc acgttcggcg
     8641 agaatctgaa gcccgaaaag ctggtcgaga tcttgaatgc ctacctgggc ctggccgccg
     8701 acgccgtgct cggcgaagaa ggcacgctcg acaaatttat gggcgacgcc gtgatgggct
     8761 tcttcaacgc gccgctcgac caggccgacc acacgttacg cgccgtgcgc gcggcgatcg
     8821 cgacgcagaa agcgatccag gaatatcgcg agcgcgtgcc atccggcgag cgcctgtctt
     8881 atggcgtcgg catcaacgtc ggcgaagccg tggtcggcaa tatcggcaac gcgcagcagc
     8941 agaactacac ggccatcggc gacagcgtga attacgccag gcgcctgcaa gagaacgccc
     9001 ggcacgatca aatcttgatc agccaggcgg tatacgatct cgtcaaagat cgcgtgcgcg
     9061 tgaatgcgct cgagccgctg ctggtcaagg gtcacagcaa gccggagccg gtgttcgagg
     9121 tgcttggact caagtaggct ttcacctgcc actgcgcgcc gcacccggcg cttgaggagg
     9181 ccgcgacgga cacacgtcgc ggctttgtgt tgccctttat cgaaaggccg ttgagactga
     9241 atgatcgtgg ctactgtctt gtatgccatc gctacgacgg ccctcgggtt atacggcgtc
     9301 tacgtgctgg cgctcgtcgc ccagtgcctt cgtcaccgcc gcgcgccgcg cctgccgctt
     9361 ccacccgtgc cgcgagacga atacgacttg ccggtcgtca cagtgcagtt gcccctgtat
     9421 aacgagccgg ccgtggccga gcgcatcgtc gacgcggcgg ccgccctcga ctggccgcgt
     9481 caccggcttc agattcaggt gcttgacgat tcgactgacg acacgacggc catggcgcgg
     9541 gcgcgcgccg agtcgcatcg gcgacgtgga atcgacgtga tgctgatcca tcgctcgagc
     9601 cgagatggtt acaaggcggg cgcgctggcg aatggactga aatcggcgcg cggcgaatac
     9661 gtcgccgttt tcgacgccga cttcgtgccc gcgccggatt ttttaaggcg cttgatgccg
     9721 tacttctccg ccagtgcgtc gatcggcttc gtgcaggcgc gctgggagca cctgaactat
     9781 caccgttcgg cgctggcgcg tgctctggcc gtcgccgtcg acgggcattt cgtcgtcgag
     9841 cagttcgcgc ggaatcgatc tggctggccg acgatcttca acggctcggc cggcatctgg
     9901 cgccggtcgt gcatcgagct cagcggcggc tggcacaccg atacactgtg cgaggacatg
     9961 gatctcagct accgcgccat gttcgctgga tggcgcggtg tgttcgtccc ggacgtatcg
    10021 gtgccgcagg aagagcccga gtcgatcggc gccgtcaagc ttcagcacgc gcgctgggcg
    10081 aagggcggcg cgcagtgcct gcgcaagcac gccctatcta tcttgcgtgc atcgtcgtgg
    10141 tcgcccatac agaaggcagc cggcttcgcg taccttgcgg gttacacggc gcacttgttg
    10201 atggtactgg tcgtgctgtt gtggctgccg ctggcgatgg gtggcccaag tcttcagcaa
    10261 ggaccgttgg cgttcctcgg cctgagtgga ctggggctgc cattcgccca cggcgtcgcg
    10321 cagtgggcgc tccaccgtgg cgacgggagc tggcttcgac ggctatctta cctgccgctc
    10381 ctgctcgtcg tcggtttcgg gatcgcgttc agcaatgcgc tggccgtgct gtccggcctg
    10441 atccggcagg gtggtgagtt tcaccgcacg ccgaaaggcg ccgcggacac tacccggctc
    10501 gctgattcgc gcccgcgcgc cgagttgctc gcctggctgg agatcgggat ggcgctgtac
    10561 gccatagccg ccgggcttgt cctgtggata gccggccatc cgacgacgtc ggcattcgtg
    10621 gcgacctttg cgctgggctt tggcagtgtg gggattgggt cgctggtcga gatcgcgcgc
    10681 gtgcgcggca atgcgccggc gcgtggtgcg cagcgcaggc gtcgcgctgc cttgtaacat
    10741 gtggacggtt cgcagcaagt cactcaaagg cgcgacgatg ccggatggca cgaagcgcga
    10801 gtgaaaggtt tgtcatgccc ggttggatca ccacggttga ggctaccgtg ttggccgggt
    10861 atgctcccag taactactta ccaattacga tttgtctact gtgcgtcgat gacctcgccg
    10921 ggcgtgactg ccgcgccgcc aggcggttcg agcatcggca tgtcaggctc gccgggcatg
    10981 atggactggc ctcgaaagaa accgccttcg tcgatcagga acgaggcgac ggtgatgtcg
    11041 ccccacacgc gacccgtcga caaaatctcg agccggccgg cggcctcgat ggtgcctttc
    11101 gccgcgcccg aaaccgacac attgtcagcg ctgatgtcgg cgatgacctt gcccgtctcg
    11161 ccgatgatca agttgcctgc gacccggatt tccccttcga aaatgccgtc gatgcgaatg
    11221 ccggcgtcgc tggtaatact ccccttgacg tgcgccgtcg agccgataac cgtctcgatc
    11281 gtgcgcgctg cgccacccgc aggcgcggac gggggtgcgg cggcaggtcg ttgctgcggt
    11341 ttctcgcgtg aaaacacggt ggatctcctc cgaataagtg atttgagcac gaggtcacat
    11401 catacacggc ttgcaggcaa tggtcaaatg atccggcttt gctcaggggg acgaggactg
    11461 gcctgcaacc gtgaccggac cgaggatgac gcggtcgcct gcgagattcc catccgctcc
    11521 gaggatgtac acgcggcggt tgctcgtcaa tccgtacagc ccggcctgga gcagatagtc
    11581 gcccggtggg acgtcgggcg gtagcgtgag cgtatacgga tcgctcacgc cctggccttc
    11641 gatccatctg ggaatccaca aatgggtggg aaacgcgccc ccgagcggcg tgtagaagtc
    11701 ggccggcggc gcggcgagct gctcccccgg cgtgatcaag tgcacgaaca ccttgtagtt
    11761 gtcgagcggc tggcgctgcc ccaaccagtc gagccagacc tgtattgact ggccggggcg
    11821 cacgacgagc ggctccgtcc atgcgcgtga tgccgcatgg accacctgcc cgttacccga
    11881 agccgatgcc ccgaccaggg cgatctgcgc cccaaaatcg gcccatgcag gcgagctcag
    11941 cgcggccggg gcaagttgag ggacctcgac gcgcagcgcg ccgaccggga ccatggagac
    12001 ttcggaaatc cccacagact tccgaagtct gaggtcgcgc ccctcggtga gatcggcgac
    12061 gccgagcgac agtggatatt cgcccggcgg cgtcgagaaa ggtagggcga tctcgtagcg
    12121 ctcgacgatc acttcgccag gacgccaatc cgtgctcagc acgcgtgaat cggtcgtcca
    12181 ccggtaagcg cgcccgccga cgtccgcgta aggcatcagg atgtgcgtcg gcgtgacggg
    12241 ggcgcgcatc gccagagtga tgtgggccgt gtcgcctgcc tgcagcgccg tgcgatcgag
    12301 cgagacgccc agcagctcga tctcgccctc ggctatcacg tcgagcgtcg tcgacaacgc
    12361 cgggagactg gcatcgccta gcgcggcgac gcgccataaa ggcggcgatt cgccgtccgg
    12421 tcgcaggcga aagagaccgc cctcgacgac ctgccgccga tagtcggcaa ggtatacggg
    12481 accgcgatcg aagtacgcga agacgttgtc tagccacgga ttgaaggagc cggcggcgac
    12541 gtacaccaag gtcacgtcgc gcggatcgag aggatggttg ttggcatact gctcgaccca
    12601 cagcggcgtg agcgcttccc aaggcgcaag caggaacgc
//
LOCUS       DBNHXB010000280         5855 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_241557, whole genome shotgun sequence.
ACCESSION   DBNHXB010000280 DBNHXB010000000
VERSION     DBNHXB010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_241557"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="AC162G_11355"
     CDS             complement(<1..765)
                     /locus_tag="AC162G_11355"
                     /inference="COORDINATES: protein motif:HMM:NF014679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR5076461.1"
                     /translation="MSRIAIVTDSSSGLSAGLIARYDLHVLPYYILAGGRMLRDGIQI
                     SRDEVFRLLRDGRHLPNTKGPSQEDYAALYRSLLGKVDAVLVILPPRERTAAYQHADS
                     ARRLIPDLPVTLLDARQTSAGQALVVLAAARARKAGGDLWAIAAAARQAGERMEFWIV
                     LESLKFAARSGRVPNIAQYIGSMVRPVPVLRHVDGAPHIAFTARSAERAIERLKDELA
                     GRVRGERVHAAIVHAGDAARAESMRAWAQSTWPAIDC"
     gene            complement(765..1415)
                     /locus_tag="AC162G_11360"
     CDS             complement(765..1415)
                     /locus_tag="AC162G_11360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR5076462.1"
                     /translation="MSIDATLLALLVIGYIFGSVPYAVIVSRARGVDIRAVGSKNPGA
                     HNVMGHVGRGWGWLVTILDLLKGALPALLAKLVGLSDWAIVAVGVAAMLGHITSPFLG
                     FKGGKGVATGFGMLLVLAPVAMVTGSVIGLTILRVTRLVALSAGIALILTFGLMIAAG
                     ASGAALAAPWIVLAISAAAAAVDEMHKTSGSLPAMLKNVLLRGASRRAQGEPSERE"
     gene            complement(1523..2620)
                     /locus_tag="AC162G_11365"
     CDS             complement(1523..2620)
                     /locus_tag="AC162G_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076463.1"
                     /translation="MTLNHWPTLLQTVLRIAVFLLGASVVFAALASAIRTFVLPRSAS
                     DFVARAVFTSLRRTFDRRVRKARTYEGRDRIMALYAPIGLLLLLAAWLVLVWIGFSGM
                     LWAAGIGSWYDALRASGSSLLTLGFAALDGLPETILAFAEAMIGLILIALLIAYLPTI
                     YSAFSRREAAVTLLEVRAGSPPSAVEMILRYNRIHGLRRLSEVWTAWEAWFADVEESH
                     TSLAALVFFRSPKPERSWVTAAGAVLDAASLVNAAIDAPRDPQADLCIRAGYIALRRI
                     AGFFRVSYDPQPRRGDPISISREEFDEACEVLAEAGVPLKPDRDLAWLDFAGWRVNYD
                     TVLLALASITMAPYAPWSSDRSVRLQRPARP"
     gene            2705..3697
                     /locus_tag="AC162G_11370"
     CDS             2705..3697
                     /locus_tag="AC162G_11370"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019154071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076464.1"
                     /translation="MPLIQVTDLHKEFRVYKHHRGARGAIRNLFTREYRIVRAVDGVT
                     FAIEAGELVGYLGPNGAGKSTTIKMLTGILVPTHGEVIVDGRVPWKQRVDHARGIGVV
                     FGQRTNLWWDLPVIESLDLLRHVYRVPEDRYERNLSHFGEMLGLDEFLHTPARSLSLG
                     QRMRADLAAALLHDPRIVFLDEPTIGLDVVAKERIRRFIQSINRERGVTVILTTHDLG
                     DVQKLCERVMMIDHGKLLFDGPPGDLVSRFGGERELVVDFAQDYEDVSVDGARIALRD
                     GRRVTYRFARGAISASELIQRLSVTYRIEDLSVQEPQIEDTVRRIYEQGLLRKT"
     gene            complement(3761..4747)
                     /locus_tag="AC162G_11375"
     CDS             complement(3761..4747)
                     /locus_tag="AC162G_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR5076465.1"
                     /translation="MNTLILTHADVEQLLPMDECVDVMAGALVALAKGQVHQPLRMMV
                     RPPDAAGLLGLMPAYRSGERAAFGLKAVSVFPGNTARGLDAHQGAVMLLSAETGELLA
                     LLNASAVTAIRTAAVSAVATNVLARQDATDLAIVGAGVQARWHLAAMAAVRPIKRVRV
                     ASRDFERARAFAAEMGFHVLAPIDAVDGVELAVENADIVVTATTASEPVLKREWIIPG
                     AHINAVGASVPAAREIDAATMAAASLFVDRRESTLNEAGDYLLAVREGAIGPEHIRAE
                     IGEVLIGAKPGRTSGGEITLFKSVGLAVEDLAAAEHVYRKAKQFGVGTWVEF"
     gene            5063..5209
                     /locus_tag="AC162G_11380"
     CDS             5063..5209
                     /locus_tag="AC162G_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR5076466.1"
                     /translation="MSDNPIAVGQPAPDFSLAASAGLSPLSLAGLRGKTVVLAFYVLD
                     FTDT"
     gene            5222..5533
                     /locus_tag="AC162G_11385"
                     /pseudo
     CDS             5222..5533
                     /locus_tag="AC162G_11385"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            5671..>5855
                     /locus_tag="AC162G_11390"
     CDS             5671..>5855
                     /locus_tag="AC162G_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076467.1"
                     /translation="MLAILFWLHLMGISIWVGASFLMPLVIVPSVQAGIDPPARLKFM
                     LALADRLLRWILLSIAVV"
BASE COUNT         1026 a         1964 c         1942 g          923 t
ORIGIN      
        1 gcagtcgatc gccggccagg tcgattgcgc ccaggcgcgc atcgactcgg cccgcgcggc
       61 gtcgcccgcg tgcacgatgg ccgcgtgaac gcgctcgccc cgcacgcggc cggccagctc
      121 gtccttcagc cgctcgatcg cgcgctcggc gctgcgcgca gtgaaggcga tgtgcggcgc
      181 gccgtcgacg tggcgcaaga ccggcaccgg cctcaccatc gacccgatgt actgcgcgat
      241 gttcggcaca cggcccgagc gcgccgcaaa tttcagtgac tccagcacga tccagaactc
      301 catgcgctcg cccgcctgcc gcgcggcagc ggcgatcgcc cacaggtcgc cgcctgcttt
      361 acgcgcccgc gccgccgcca gcacgaccag cgcctgcccg gccgacgtct gccgcgcgtc
      421 gagcagcgtg accggaagat ccgggatcag ccgccgcgcc gagtcggcgt gctgatacgc
      481 cgccgttcgc tcgcgcggcg gcaggatgac cagcacggca tcgactttgc ccaacagcga
      541 tcgatacagc gcggcgtagt cttcttgcga agggcctttc gtgttcggaa ggtgacgccc
      601 gtcgcgcagc aggcggaaca cctcgtcgcg cgagatctga atcccatcgc gcagcatgcg
      661 cccgccggcg aggatgtagt aggggaggac gtgcagatcg tagcgcgcga tcagcccggc
      721 gctcaacccg ctgctgctgt cggtgacgat cgcgatgcgc gacattactc tcgctctgat
      781 ggctcgcctt gcgccctgcg cgacgcgcct cgcaaaagaa cgttcttcaa catcgccggg
      841 aggctgcccg atgttttgtg catctcgtcg accgcggcgg cggcggcgga gatcgccaga
      901 acgatccagg gcgcggccag cgccgccccg ctcgctcccg ccgcgatcat cagcccgaat
      961 gtcaggatca aggcgattcc ggcggagagg gcgacgaggc gcgtcacgcg caagatggtc
     1021 aggccgatga cactgccggt gaccatggcc accggcgcca gcacgagcag catgccgaag
     1081 ccggtggcga cgcccttccc gcctttgaat cccaagaacg gcgaggtgat gtggcccagc
     1141 atcgccgcca ccccgacggc cacgatggcc caatcggaaa gtcctaccag tttagccaac
     1201 agcgcaggca gcgccccctt gagcagatcg aggatcgtga cgagccagcc ccatccgcgc
     1261 ccgacgtgtc ccatgacgtt gtgcgcgccg ggattcttcg acccgacggc gcggatgtcc
     1321 acaccccgcg cgcgggagac gatcaccgcg tacgggaccg atccgaagat gtagccgatc
     1381 acgagcaagg ccagcagcgt ggcgtcaatc gacatacggc actgattgta gcatggtcga
     1441 gggagaatgg gattcgtggc agacctgcca ggtcttggcg atccatagca tggctggccc
     1501 tgacaaatga gacctagcag gtctaagggc gcgcggggcg ctgcaggcgg acggatcgat
     1561 ccgacgacca gggcgcgtac ggcgccatcg tgatcgaggc cagcgccagc agcaccgtgt
     1621 cgtagttcac gcgccagccg gcgaagtcga gccaggccag atcgcgatcg ggcttgagcg
     1681 gcacgccggc ttcggccaac acctcgcagg cctcgtcgaa ttcctcgcgg ctgatgctga
     1741 tcgggtcgcc ccgcctgggt tggggatcgt acgagactcg aaagaagccg gcgatgcggc
     1801 gcagcgcgat gtagccggcg cggatgcaca ggtcggcctg cggatcgcgc ggcgcgtcga
     1861 tcgccgcgtt gaccagcgac gccgcgtcga gcactgcgcc cgccgccgtc acccacgaac
     1921 gctcgggttt cggcgagcgg aagaacacca acgccgcgag cgaggtgtgg ctttcctcga
     1981 cgtccgcgaa ccacgcctcc cacgccgtcc agacttcgct caggcgacgc aagccgtgaa
     2041 tgcggttgta gcgcaggatc atctcgaccg ccgacggcgg tgatccggcg cgcacttcga
     2101 gcagcgtcac cgccgcctcg cgccgcgaga aggccgagta gatcgtcggc aggtacgcaa
     2161 tcagcagcgc gatcagaatc agtccgatca tggcttcggc gaaggccagg atcgtctccg
     2221 gcaggccgtc cagcgcggcg aagccgagcg taagcagcga cgagccgctg gcgcgcagcg
     2281 cgtcgtacca cgacccgatg ccggctgccc acaacatgcc ggagaacccg atccacacca
     2341 ggaccagcca cgccgcgagc aagagcagca agccgatcgg cgcgtagagc gccataatgc
     2401 ggtcgcgccc ctcatacgtg cgcgcttttc gcacgcgtcg atcgaacgtc cggcgcagcg
     2461 acgtgaacac ggcgcgggcg acgaagtcgc tcgcgctgcg cgggaggacg aacgttcgaa
     2521 tcgccgaggc cagcgcggca aagacgacgg atgcgccgag gaggaagacg gcgatgcgaa
     2581 gcacggtctg gagaagggtg ggccagtggt tgagagtcat cgcgccgaat tatacccgct
     2641 cggccatcgc cgcgcggcag atcgtggtaa actcgctctc aagggagggg gtgggtgagg
     2701 gtatatgcct ctcatccagg ttaccgatct ccacaaggaa ttccgcgttt acaagcacca
     2761 tcgaggcgcg cgcggcgcga tccgcaacct gttcacgcgc gagtatcgca tcgtgcgcgc
     2821 cgtcgacggc gtgacgttcg ccatcgaggc gggcgagctg gtgggctacc tcggcccgaa
     2881 cggcgctggc aaatcgacga cgatcaagat gctcaccggt atcctcgtcc cgacgcacgg
     2941 cgaggtgatc gtcgacgggc gcgtgccgtg gaagcagcgc gtcgaccacg cgcgcggaat
     3001 cggcgtcgtg ttcggccagc gcaccaacct gtggtgggac ctgccggtca tcgagtcgct
     3061 cgacctgctg cgacacgtgt accgcgtgcc ggaggatcgc tacgagcgca acctgtcaca
     3121 cttcggcgag atgctggggt tggacgagtt tctgcacacg cctgcccgct cgctgtcgct
     3181 cggccagcgc atgcgcgccg atctggccgc tgcgctgctg cacgatccgc gcatcgtgtt
     3241 cctcgacgag ccgacgatcg ggctggacgt cgtggccaag gagcgcatcc ggcgcttcat
     3301 ccagtcgatc aatcgcgagc gcggggtgac cgtgatcctg acgacgcacg acctgggtga
     3361 cgtgcagaaa ctgtgcgagc gggtgatgat gatcgatcac ggcaagctgc tgttcgacgg
     3421 gccgccgggc gatctggtga gccgcttcgg cggcgagcgc gagctggtcg tggacttcgc
     3481 gcaggactac gaggacgtgt ccgtcgacgg agcgcgcatc gcgctacgcg acgggcggcg
     3541 cgtcacgtat cgtttcgcac gcggcgcgat cagcgcctcg gagctgatcc agcgcctgag
     3601 cgtgacgtat cgcatcgaag atttgagcgt gcaggagccg cagattgagg atacggtgag
     3661 gcggatttac gagcaggggt tgttgagaaa gacgtaggac aggtttccat acctgcctga
     3721 aagaaggcgg ctttcataag ccgccctacg ccgagaaaac tcaaaactcc acccacgtcc
     3781 ccacgccaaa ctgtttcgcc ttgcgataga cgtgctcggc tgcggcgagg tcttcgacgg
     3841 ccagcccgac cgacttgaac agcgtgatct cgccgccgga tgtccggccc ggtttcgcgc
     3901 cgatcagcac ttcgccgatc tcggctctga tgtgctccgg tccgatcgcg ccctcgcgca
     3961 ccgccaacag gtaatcgccg gcctcgttga gcgtcgactc gcgccggtcg acgaacagac
     4021 tggccgcggc catcgtcgcc gcgtcgatct cgcgcgccgc cgggacgctt gccccgacgg
     4081 cgttgatgtg cgcgccgggg atgatccact cgcgcttcaa taccggctcg ctggccgtcg
     4141 tcgccgtgac gacgatgtct gcattttcga cggccagctc gacaccgtcc accgcgtcga
     4201 tcggcgccag cacgtggaat cccatctcgg cggcgaaggc gcgcgcgcgc tcgaaatcgc
     4261 ggctggcgac acgcacgcgc ttgatagggc ggacggcggc catcgcggcg aggtgccagc
     4321 gcgcctgcac gccggcgccg acgatcgcca ggtcggtcgc gtcctggcgc gccagcacat
     4381 tcgtcgcgac ggctgagacg gcggcggtgc gaatcgccgt gacggcagag gcgttcagca
     4441 gcgcgagtag ctcgcccgtc tcggcgctga gcagcatcac cgcgccctgg tgcgcgtcca
     4501 gacccctggc cgtgttgccg gggaacacag acactgcttt caacccgaag gccgcgcgct
     4561 cgccggatcg gtacgcgggc atcaggccca acagcccggc ggcatcgggc gggcgcacca
     4621 tcatgcgcag cggctgatgc acctgccctt ttgccagcgc gaccagcgcg ccggccatca
     4681 cgtcgacgca ttcgtccatc ggcagcaact gctcgacatc ggcgtgagtg aggatgagtg
     4741 tgttcatcga caagctccca tgtagcagtt tccgattgcg ggtacgccgc tccggaatcg
     4801 agccttcagg cggtttccgg ccaatgcctc gactccggcg taagccaaag tggaagccgc
     4861 tccaagccgg tgaaactgcg cgccaatgct cggcgcagtt tgcctttcgt gacgagtagt
     4921 gtatcatcta gcgcactctc ctgcaacgca ccatccgaga gcctatctgg aaatcccgta
     4981 gcgggcgcat ccctatgcgc cagcaagcgg catggggatg ccgccctacg gttttcggat
     5041 gggtgctgag gaggaaccga gaatgtctga caacccgatc gccgttgggc agcccgcgcc
     5101 cgacttttca ctcgcggcga gcgccggcct cagcccgctg agcctggccg gcctgcgcgg
     5161 caagaccgtc gtgctcgcct tctacgtgct cgacttcacc gacacttgaa ggacggagct
     5221 tgcctcgttt cagaagaacc tctctcgatt cgaggcggcg aatgcccagg tgctgggcat
     5281 cagcgtggac agtgtgcact cgcatcgcgc cttcgccagg gagctgggcg gcatcgactt
     5341 ccccctgctg gccgatttcc atccgaaagg cgaagtgacc aaagcctacg gcctgtggcg
     5401 cgacgacaga ggctgcagcc ggcgtgcgat cgtgatcgtc gatcgcgaag gcatcgtccg
     5461 atacgtcgag gtgatcccgc aaggtcctcc ggacgtggag aaggtgctga acgaggtcaa
     5521 agccatctcg taaccacgga tttccacccc cgcacacagc aggcgagggc aggcggatca
     5581 cacagatatt gatgggccgc catccgtagt gaacaatctt cgcggccgcg cagtgtctgg
     5641 gaggcaatac tacaagcagg gaggaatgcc atgcttgcca tcctgttctg gctgcacttg
     5701 atgggcattt ccatttgggt cggcgcgtcg ttcctgatgc cgctggtgat cgtgccgagc
     5761 gtgcaggccg ggatcgaccc gcccgcgcgc ctgaaattca tgctcgcgct ggccgaccgg
     5821 ttgctcagat ggatcctgct gtcgatcgcc gtggt
//
LOCUS       DBNHXB010000281         6456 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4099940, whole genome shotgun sequence.
ACCESSION   DBNHXB010000281 DBNHXB010000000
VERSION     DBNHXB010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4099940"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1655)
                     /locus_tag="AC162G_11395"
     CDS             complement(<1..1655)
                     /locus_tag="AC162G_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HIR5076468.1"
                     /translation="MSELRGARIHITGVVQGVGFRPFVYGLATRLSLSGWVRNTSAGV
                     DIEADGSPEVLDQFVRALRDEAPPLARIDSMQVEARRANGFTTFEIVHSEPVPGAFLP
                     VSPDVCVCADCLREMRDPGDRRYRYPFINCTNCGPRFTIIKDIPYDRPLTTMAPFEMC
                     PECAAEYADPLDRRFHAQPVACPACGPHVWLEMNLDADERGYTRIKEEKIGVHQRLSA
                     SLYPLTGDAAIQEAQRLLAQGKIVAVKGLGGFHLACDATNDAAVTELRRRKGRIDKPF
                     ALMMPDVETVERFCEVSAEERALLESPQRPIVLLRRRSGEPTAQRISDAVAPHQSTLG
                     VMLPYTPLHYLLMANGVSHIAYRISRMADGEWEDSEDQPSAISHQLSAIVLTSGNFNE
                     EPIATDNEDALTRLASLADAFLLHNRDIHVRCDDSVVRVFAPTPDPSPARGGGDGVGV
                     TILRRSRGYAPYPVRLPFDAPPILAAGAELKNTFCVTREGYAFLSHHIGDMENYETLR
                     SFEDGVAHFERLFRIQPEVIAYDLHPNYLATRYALERAGRDGIR"
     gene            complement(1747..2118)
                     /gene="hypA"
                     /locus_tag="AC162G_11400"
     CDS             complement(1747..2118)
                     /gene="hypA"
                     /locus_tag="AC162G_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013334.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HIR5076469.1"
                     /translation="MHELPVVENILEIALQRAKEAGAKRITDLHLVVGPLSSVVDEPV
                     QFYWDMVSKGTPAEGARLHFRRVPIELECLACGLRYTGGEDDLACPACGSGRIKALAG
                     EEFYLEAIDVDAGEPVPAAQA"
     gene            complement(2348..4150)
                     /locus_tag="AC162G_11405"
     CDS             complement(2348..4150)
                     /locus_tag="AC162G_11405"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5076470.1"
                     /translation="MIDPDGSPGRPDVPAAADLIHSNARLIRMRWLAGLAVLAGTVFG
                     VHVAGAPLPALSLYVLGAIILAYNALVEYASRRAGTRRPALITTVQIAFDWLALAAFV
                     HFTGGIESPAIAFFLFYVLLASILLPGRTAFTYAVLVIGLVFILVVLEAAGVLPHYHV
                     IPALPESLYRDPLYVTAILAFFGVTLLVIVSLAAPVLHEFRDHQRRLATLYQSVQALP
                     SSLDLPRVLDQLVHGVTHALQAKAASIRLLDETGRQLKVTAAYGLSQAYLEKGPVEVD
                     RSPIDQEALRGRPIIVEDTAGDPRLQYPAEVLAEGIHSILCVALIGRRRPLGVLRVYS
                     WQPRFFTEKDIDFVTTVARQGAMAIENAMSYGELQRADATKSQFVRAVTHELRSPVVA
                     SQSLLRTVIRDLAGGLNDLQRDILGRLSERLNVLQMLIDDLLDLAAGKVEGLEGALTP
                     VSVEAMVLGIVDRLSSQAEEKKVDLRVDYVPRGLSVMASEDGLSRIFLNLIGNAVKYT
                     PAGGKVDVELEQRGDDVVVTVADTGIGIPEVDVPHIFEEFYRASNVKQNGLTGTGLGL
                     AIVKDLVERYGGRVSVRSKPGEGSTFTVVLPLAG"
     gene            complement(4151..4567)
                     /locus_tag="AC162G_11410"
     CDS             complement(4151..4567)
                     /locus_tag="AC162G_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5076471.1"
                     /translation="MAKILIIDDDPDIVLAMRLCLKGAGHEVFDAKNGQDGLRMVPEI
                     KPDLIILDVMMDTTTEGFQVSLKLRSPDPGSPLAAYSRIPILMLTALHSTTDLRFGPD
                     KDYLPVDAFVDKPIDPDKLVRQVDAMLAKSRQPEGS"
     gene            complement(4652..5119)
                     /locus_tag="AC162G_11415"
     CDS             complement(4652..5119)
                     /locus_tag="AC162G_11415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HIR5076472.1"
                     /translation="MSLPAKQTLVLGLGNPLRGDDGLGPAVIRWMQARGLPRGATAID
                     AGTRGLDVLLTLAEYRRVFVVDAAGVGRAPGEWLRIAPDLRRLGVETPALSCHAAGLV
                     DALALGAALDVLPEQVIIFGVQPARVDWSPELSAEVRKAVPAVGRAILSEIGR"
     gene            complement(5176..>6456)
                     /locus_tag="AC162G_11420"
     CDS             complement(5176..>6456)
                     /locus_tag="AC162G_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HIR5076473.1"
                     /translation="VHHLTRVEGHGNIVVDVERGELKRCELQIVETPRFFEAMLVGRP
                     YAEASPIASRICGICSVGHATTSLLAVEAALGVTPSEQTQLLRKLNFLGEILDSHILH
                     AYMLVAPDLLGVGSVVPLARSHRDVVLRALRMKKLAGDLCAVVGGRHTHPLTLTVGGF
                     TRLPYRSDLEELRARLEAARQDVDATVELFQSLALPDFERETEYVALRQPGEYAFVGV
                     AGATITSSDGYQMHVADYGELTNEFLVPHSTAKHTRHRRDAYMVGALARFNVNYDQLC
                     ARAQAAAATLGLKPACINPYLNTIAQVVEIAHCVESAITLVDALLERGVQPEAPPPVE
                     VQAGEGVGACEVPRGTLYHHYRIGDDGRLTGANCIIPTGQNLANIEADMRTLVPRLLD
                     RPQYEVRLALEMLVRAYDPCISCSTHMLDVTFEV"
BASE COUNT         1146 a         2138 c         2061 g         1111 t
ORIGIN      
        1 cgaatgccgt ctcgccccgc ccgttcgagc gcgtagcggg tggcgagata gttggggtgc
       61 agatcgtagg cgatgacctc gggctggatt cggaacaggc gctcgaagtg cgccacgcca
      121 tcctcgaacg agcgcagcgt ctcgtagttc tccatgtcgc cgatgtggtg actgagaaag
      181 gcatatccct cccgcgtcac gcagaaggtg ttcttcagct cggccccggc ggcaaggatc
      241 ggcggcgcgt cgaacggcag ccggaccggg tacggcgcgt atccgcgcga gcggcggagg
      301 atggtgaccc ccaccccatc ccctcccccg cgcgcggggg aggggtcagg ggtgggggcg
      361 aacacgcgca cgacggaatc gtcgcagcgc acgtgaatgt cgcggttgtg taggaggaac
      421 gcgtcggcta acgaagcaag ccgagtcaga gcatcttcat tatccgtcgc gatcggctcc
      481 tcgttgaagt tgccgctggt gagcacgatg gccgatagct ggtggctgat agccgatggt
      541 tgatcttcgg aatcctccca ttcaccatct gccatccgcg atatgcgata tgcgatatgc
      601 gatacgccat tcgccatcag caagtagtgc aacggcgtgt agggcagcat gacgccgagt
      661 gtggattggt ggggagcgac cgcgtcggag atgcgctggg cggtgggttc gccggatcgc
      721 ctgcgcaaga ggacgatcgg gcgctgcggc gattcgagca gtgcgcgctc ttcggcggat
      781 acttcgcaga accgctcgac ggtctcgacg tcgggcatca tcagcgcgaa gggcttgtcg
      841 atgcgcccct tgcggcggcg cagttcggtc acggcggcgt cgttcgtcgc gtcgcacgcc
      901 aggtggaagc cgcccagacc cttgacggcg acgattttgc cttgcgccag caagcgttgt
      961 gcttcctgga tcgcagcgtc accggtcaat ggataaagag acgcggataa acgctgatga
     1021 acgccgattt tttcctcttt tatccgcgtg tatccgcgtt catccgcgtc caaattcatt
     1081 tccagccaga cgtgaggccc acaggccggg caggccaccg gctgcgcgtg gaagcggcga
     1141 tcgagcgggt cggcgtattc ggcggcgcac tcggggcaca tctcgaacgg cgccatcgtc
     1201 gtgagcgggc ggtcgtacgg gatgtccttg atgatcgtga agcgcggccc gcagttggtg
     1261 cagttgatga acgggtagcg atagcgccga tcgcccggat cgcgcatctc gcgcaggcag
     1321 tccgcgcaca cgcacacgtc aggcgagacc ggcaggaacg cgccgggcac aggctccgag
     1381 tgaacgatct cgaaggtcgt gaagccgttg gcgcggcgcg cctcgacctg catcgagtcg
     1441 atgcgcgcga gcggcggcgc ttcgtcgcgc agcgcccgca cgaactgatc caggacttcg
     1501 ggcgatccgt cggcctcgat gtcgacgccg gccgacgtgt tgcgcaccca gccgctcagc
     1561 gacagccgcg tcgccaggcc atacacgaac ggccggaagc cgacgccctg cacgacgccg
     1621 gtgatatgga ttcgcgctcc tcgcaattca ctcatagtgg tgacaaccag gatcttgcca
     1681 cggaggcact gaggcacgga gaagtgatcg ttgtaatcct ctgtgtctcc gtgtctccgt
     1741 ggctaatcac gcttgcgccg ccggaacagg ctcgcccgcg tcgacgtcga tcgcttcgag
     1801 atagaactcc tcgcccgcca gcgccttgat ccggccgctg ccgcacgccg ggcaggcgag
     1861 gtcgtcctcg ccgcccgtat accgcagacc gcacgccagg cactctaact cgatcggcac
     1921 gcggcggaag tgcaggcgcg ccccttcggc aggcgtccct ttactcacca tgtcccagta
     1981 gaactgcacc ggctcgtcga cgactgaaga cagcgggccg acgacgaggt gcagatcggt
     2041 gatgcgcttc gcgccggcct ctttcgcgcg ctgcaaggcg atctccagga tgttttcgac
     2101 gacaggcagc tcgtgcatgg tttttcctcc accctacata atactcgact ttgtcggtgg
     2161 ctcaagtaac atctgtcatg cctctattgc ccattcgata cacgacacac ggatttccca
     2221 ctttggcgct ggttcacaat tagatttcga tccaccggtg tcaacgaata tgcaacgaat
     2281 caacgaatgc gggggcgcat ccgttgattc gttgtctatc cgatgacgcc gtcctaccag
     2341 tcctttgtca gcctgccaac ggcagaacga cggtgaatgt ggagccttcg ccgggcttgc
     2401 tgcgcacgga gacgcgcccg ccgtagcgct cgaccaggtc cttcacgata gccagcccca
     2461 ggcccgtgcc ggtcaatccg ttttgcttga cgttgctggc tcgatagaac tcctcaaaga
     2521 tgtgcggcac atccacctcg gggatgccga tgcctgtgtc ggcgacggtg acgaccacgt
     2581 cgtcgccgcg ctgctccaac tcgacgtcga ctttcccccc tgcaggcgtg tacttcaccg
     2641 cgttgccgat caggttgaga aagatccggc tcaggccgtc ttcgctcgcc atcacggata
     2701 ggccgcgcgg cacgtagtcc actcgaagat cgaccttctt ctcttccgcc tggctcgaca
     2761 ggcgatcgac gatgccgagc accatcgctt cgacggatac cggcgtcagc gcgccttcca
     2821 ggccctcgac tttgccggcc gccaggtcca gcagatcgtc gatcagcatt tgcagcacgt
     2881 tgagccgctc cgacaggcgg cccagaatgt cgcgctgcag gtcgttcagg ccgccggcca
     2941 ggtcgcgtat cacggtgcgc agcaggctct gcgacgcgac gacgggcgag cgcagctcgt
     3001 gcgtcaccgc gcgcacgaac tgcgacttcg tcgcgtccgc gcgctgcagc tcgccgtaac
     3061 tcatcgcgtt ttcgatagcc atcgcgccct ggcgcgccac tgtcgtcacg aagtcgatat
     3121 ccttctcggt gaagaaacgg ggctgccaac tgtagacgcg cagcacgccc agcggccggc
     3181 gccgcccgat cagcgccacg cacaagatcg aatggatgcc ctcggccagg acttcggccg
     3241 gatattggag tcgcgggtcg ccggccgtat cttcgacgat gatcggccgg ccgcgcagcg
     3301 cttcctggtc gattgggctt cggtcgacct ccaccggccc tttctccagg tacgcctggc
     3361 tcagaccgta ggcggcggtc actttcaact gccggccggt ctcatccagc aggcgaatgg
     3421 aagccgcttt cgcctgaagc gcgtgcgtca cgccatgcac aagctggtcg aggacgcgcg
     3481 gtagatcgag gctgctcggc aaagcctgga cgctctgata cagcgtcgcg agccgccgct
     3541 ggtgatcgcg aaattcgtgc agcaccgggg ccgcgagact gacgatgacc aggagcgtca
     3601 cgccaaagaa ggccaggatg gcggtgacgt agagtggatc gcgatacagg ctttccggca
     3661 gggcgggtat cacgtggtag tgcggcaaca cgccggccgc ctccaagacc acgaggatga
     3721 agaccaggcc gatcaccagg acggcgtacg tgaacgccgt gcgacccggc agcaggatcg
     3781 aagccagcag cacgtagaag agaaagaagg cgatggccgg actttcgatg ccgccggtga
     3841 aatgcacgaa cgcggccagc gccagccagt cgaaggcgat ttgaaccgtt gtgatcaggg
     3901 ccggccggcg tgtgccggcg cggcgcgagg cgtactcgac cagcgcatta taagccagga
     3961 tgatcgcccc cagcacgtat agcgacagcg caggcagagg cgctcccgcc acgtgcacac
     4021 cgaacaccgt gcccgccagc acggcaagcc ccgccagcca gcgcatgcgg atcaggcgtg
     4081 cgttgctatg gatcaggtct gccgccgcgg gcacgtcggg ccgcccgggc gatccatccg
     4141 ggtctatcat tcacgagcct tccggctgcc gactcttggc gagcatggca tccacctgcc
     4201 ggacgagctt gtcggggtcg atcggcttgt ccacgaacgc gtcgaccggc aggtagtcct
     4261 tatccggccc gaagcgcagg tcggtcgtgc tgtgcaaggc cgtgagcatc aggatcggga
     4321 tgcggctgta agccgccagc ggcgagccgg gatctggact gcgcagcttg agcgacacct
     4381 ggaagccctc ggtcgtcgtg tccatcatca cgtcgaggat gatcaagtcc ggcttgatct
     4441 cgggcaccat gcgcaggccg tcttgcccat tcttcgcgtc gaacacctcg tgacccgcgc
     4501 ctttcaggca cagtcgcatg gcgaggacga tgtcaggatc gtcgtcgatg atcaggattt
     4561 tcgccattgt tactcccaag acccttgcga aggtttgaca cgcagcagct ttgttggcga
     4621 acctgcgcca aaccttcgca agagtgagta gttacctccc tatttcgctc agaatagcgc
     4681 ggcccactgc gggcacggcc ttccgaactt cggcactcag ttcaggcgac cagtcgaccc
     4741 gcgccggctg gacgccgaag atgatcacct gctcgggcag cacgtccagg gctgcgccca
     4801 acgccagcgc gtcgacgagg cctgcggcgt ggcacgacag cgccggcgtc tcgacgccta
     4861 gtcgacgcag gtcaggcgcg atgcgcagcc actcgcccgg cgcgcgcccg acgccggctg
     4921 cgtcgacgac gaacacgcgg cgatactccg cgagcgtcaa gagcacatcc aggcctcgcg
     4981 taccagcgtc gatcgccgtc gcgccgcgcg gcaagccccg cgcctgcatc caccttatca
     5041 cagccgggcc gaggccatca tccccgcgca agggattgcc cagccccaga accagcgtct
     5101 gttttgcggg caggctcact cgcacctcca acttccaacc tccaacctcc aatctccaac
     5161 ctcaaacctc caacttcaaa cttcaaaagt cacgtccagc atgtgcgtcg agcacgagat
     5221 gcacggatcg taggcgcgca ccagcatctc cagcgccagc ctgacctcgt attgcggccg
     5281 atcgagcagg cgaggcacga gcgtgcgcat gtcggcttcg atgttcgcca ggttctgccc
     5341 cgtcggaatg atgcagtttg cgccggtgag gcggccgtcg tcgccgatcc gatagtgatg
     5401 atacagcgtg ccgcgcggaa cttcgcacgc gcccacgccc tcgcccgctt gcacttcgac
     5461 cggcggcgga gcctcgggct ggacgccgcg ctccagcagc gcatcgacga gtgtgatagc
     5521 cgactcgacg cagtgcgcga tctcgaccac ctgggcgatc gtgttcaggt acgggttgat
     5581 gcacgccggc ttcaatccca gcgtcgcggc cgccgcctgg gcgcgcgcgc acaactggtc
     5641 gtagttgacg ttgaatcgcg ccagcgcgcc gaccatgtac gcgtcgcggc gatgccgggt
     5701 atgtttcgcg gtcgaatgcg gcaccagaaa ttcgttcgtc agctcgccat agtcggcgac
     5761 gtgcatctgg tagccgtcgc tcgacgtgat ggtcgcgccg gcgacgccga cgaaagcata
     5821 ctcgccgggc tggcgcagcg caacgtactc ggtctcgcgc tcgaaatccg gcagtgcgag
     5881 cgattggaac aattcgaccg tggcatccac atcctggcga gcggcttcca ggcgggcgcg
     5941 cagctcctcc aggtcgctcc ggtacgggag gcgcgtgaac cctccgacag tcagtgtgag
     6001 cggatgcgtg tggcgcccgc cgacgacggc gcacaggtcg cccgccagct tcttcatgcg
     6061 cagcgcgcgc agcacgacgt cccgatggct gcgcgccagc ggcaccacgc tgcccacgcc
     6121 gagcagatcc ggcgcgacca gcatgtacgc gtggaggatg tggctgtcca ggatctcgcc
     6181 gagaaagttc agcttgcgca gcaactgcgt ctgctcggaa ggcgtcacgc ccagcgcggc
     6241 ctcgaccgcc agcagcgagg tcgtcgcgtg cccgacggag cagataccgc aaatgcgcga
     6301 cgcgatgggt gaggcttcgg cgtacggtcg cccgacgagc atcgcctcga agaagcgcgg
     6361 cgtctcgacg atctgcagct cgcagcgttt cagctcgccg cgctcgacgt cgacgacgat
     6421 gttgccgtgc ccctcgacgc gggtcagatg atgaac
//
LOCUS       DBNHXB010000282         4136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3261930, whole genome shotgun sequence.
ACCESSION   DBNHXB010000282 DBNHXB010000000
VERSION     DBNHXB010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3261930"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..835)
                     /locus_tag="AC162G_11425"
     CDS             complement(77..835)
                     /locus_tag="AC162G_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076474.1"
                     /translation="MNCGPLSKKQEHRLPHDPADVGDMWVWRAIALPSRLRVVSHVSH
                     ERSEQEATAFLAKFKAKTDGSAPFFTSDQLPAYVAALIANYSTPEPPPAKRGPGRPRK
                     EPKRIMDPNLRYAQVDKRREGGRVVEVKRHILFGTETDIIQILEVDGCGSQINTSYVE
                     RDNLTSRHSNGRLVRKTLSHSKKKDYLERHLDLEDAIYNFVRPHRALRVRLHRPAAHG
                     RRWKQRTPAMAAGLTDHIWTVEELLTFRISHASG"
     gene            complement(786..1187)
                     /locus_tag="AC162G_11430"
     CDS             complement(786..1187)
                     /locus_tag="AC162G_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="HIR5076475.1"
                     /translation="MELERQWCDNETCSYGGEVGAGNIKVFSHVERRYYCTSCGRTFS
                     ADKGTCFETIRRPHAVVTEALALLNERNSLRAVERLKHLSPNRVLHWLDLAGHHVARV
                     SANLIRDLHLSQVQIDELWTFVKKTRAPSAA"
     gene            1416..2711
                     /locus_tag="AC162G_11435"
     CDS             1416..2711
                     /locus_tag="AC162G_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076476.1"
                     /translation="MTDPLRERLRISGEHLDDINALLLDPDARVINAFLDVVSKYGTP
                     EEINRKADEARKLPNLLARLKAIDSPYVDDLEWLIEQRDKGTFISEADYRRSVLGPRA
                     DTMAFADDFAVTLEISAFQYFPWLITEAKQAIANGELMPGRFIRVRKMKESEADDGDL
                     LAVAAAMQIVGASYVETLDTKGTYGSNVHLGGPATITGYFGGIGQPNEHALKWLDEFL
                     YYYTTYGVKQVLNINPGTVLLGYLLHKLGVDNEFKISVFMGNDNPYAALWTLIGARLF
                     SRPDGTSPLIGFNWSNSVNNETMEITAEFRKALDFEHIVRFEHHIVETWKSIVRQPYD
                     RRDELIEIAGHVANISAKHEGGDPEVERTRKHPSDILDYFREKAEVLQSGEMALLERN
                     YLDKHAALNHTARALTEHGLSFVAARHLHHVPAKERVSA"
     gene            2832..3182
                     /locus_tag="AC162G_11440"
     CDS             2832..3182
                     /locus_tag="AC162G_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076477.1"
                     /translation="MNEPSTSHRPGPLRVIVLAATGQDVRKCWHCDACNTNLAPDQDV
                     TLEILLQMVVMNDEEALTSRTLWSDAALASAPKVCSNGLDIEAVMLALRREAQRRGLH
                     PPIANYQLPKDRGD"
     gene            3183..3989
                     /locus_tag="AC162G_11445"
     CDS             3183..3989
                     /locus_tag="AC162G_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5076478.1"
                     /translation="MKLAITGKGGVGKTTLAALLAQVYADAGRVVLAADADPSPCLAG
                     ALGFPAELRARLRPIAEMDDLIEERTGAKPGTTGGFFKINPRVDDIPDRFSVSHRGVR
                     LLEMGAVDLGGSGCICPESAMLKTLFTHLLFRKDEVLILDMYAGVEHLGRATVDFVDA
                     MLVVVEPTRRSLSTAAQIKELASHIGLTRLWLVGNKVRTTEDRAFVEANSPGLPVLGH
                     LPYTPLAQEADRAGAAAYDMIPEIVEAARSIAATLEKELVAQAASATGQP"
BASE COUNT          852 a         1233 c         1283 g          768 t
ORIGIN      
        1 aaaacgggtc gaatttgaaa taggcccgaa aatttaagcg aaatggcagt gtatacgtaa
       61 gccgtgaaag gacattttag ccgctagcgt gtgaaatgcg aaaggtcagc aactcttcta
      121 cagtccagat gtggtccgtc aatccagccg ccatggcggg cgtccgctgt ttccagcggc
      181 gaccatgggc ggcgggtctg tgcagtcgaa cccgcaaggc gcgatgcgga cgcacgaagt
      241 tgtagatggc gtcttccaga tcgagatggc gttcgaggta gtccttcttc ttggagtgcg
      301 acaacgtctt gcgtaccagt cggccattgc tgtgacgcga agtcaggtta tcgcgttcca
      361 cgtaagacgt gttgatctgt gagccgcagc catcgacttc cagaatctgg atgatgtcag
      421 tttccgtgcc gaacaagatg tgtcgcttga cttcgaccac acgcccacct tcgcgccgct
      481 tgtcgacttg ggcatagcgc aagttcgggt ccatgatccg tttcggttct ttgcggggac
      541 gccccgggcc gcgcttcgcc gggggtggtt cgggtgtact gtagttggca atgagtgccg
      601 ccacataggc cgggagttga tcgctggtga agaagggagc acttccgtcg gttttggcct
      661 tgaatttcgc cagaaaggcc gtggcctcct gctcgctgcg ctcgtggctc acgtggctga
      721 ccacgcgcag tcggctgggc aaggcgatgg cccgccagac ccacatgtcg ccgacatccg
      781 ccgggtcatg cggcagacgg tgctcttgtt tttttgacaa aggtccacag ttcatcgatt
      841 tgaacttgac ttagatgcaa atcacggatg aggttcgcgc tcacacgggc cacgtggtgt
      901 ccggctaagt cgagccagtg caggacacgg ttgggtgaaa gatgcttcag acgctccacc
      961 gcacgcaggc tgttgcgctc attcaagagc gccaaggctt ccgtcactac ggcatgcgga
     1021 cggcggattg tttcaaaaca cgtgccctta tccgcactga aggttcgccc gcagctagtg
     1081 caatagtagc gccgttcaac gtggctgaag actttgatat tgcccgcacc gacttcgcca
     1141 ccgtaggaac aggtttcgtt gtcacaccat tgacgctcaa gttccatgac gtatcacctc
     1201 cgctgggtca gatgatacgc caattgcgtt tcacggctta cgtatacact caagggattt
     1261 cacggcttac atatacagca ccgtaattag tgaccaccga ttactgataa gataactggt
     1321 aactgatgac tgatgaccga tgactgataa ctgatgactg gtggctaatg gctaattacc
     1381 cataactaat aactgttccc tttacggagg tgaacatgac agacccactg cgcgagcgac
     1441 tgcgcatttc cggagaacac ctcgacgaca tcaacgcgct gcttctggac ccggatgcgc
     1501 gggtgatcaa cgcctttctc gacgtggtga gtaaatatgg cacgcccgag gagatcaacc
     1561 gcaaggccga cgaggcgcgc aagctgccga atctgttagc tcggctgaag gccatcgact
     1621 caccgtacgt ggacgatctc gaatggctga tcgagcagcg ggacaaaggc acattcatca
     1681 gcgaggcgga ctatcgccgc agtgtcctcg gcccgcgcgc cgacactatg gccttcgccg
     1741 acgatttcgc cgtcacgctg gagattagcg ccttccagta tttcccgtgg ctaatcaccg
     1801 aagccaaaca ggcgatcgcc aacggcgagc tgatgccggg ccgcttcatt cgtgtgcgca
     1861 agatgaagga gtccgaggcc gacgacgggg acctgctggc cgtggcggct gcgatgcaga
     1921 tcgtcggagc gagctacgtg gagacgctcg acaccaaagg cacatacggc tcgaacgtgc
     1981 acctgggcgg cccggcgacc atcaccggct acttcggcgg tatcggtcaa ccgaacgagc
     2041 acgccctgaa atggctggac gagttcctat actactacac cacctacggc gtcaagcagg
     2101 tgctcaacat caatcccggc acggtgctgc tcggctattt gctacacaag ctcggcgtgg
     2161 acaacgagtt caagatatcg gtcttcatgg gcaacgacaa tccctacgcc gcgctgtgga
     2221 cgctgatcgg cgcgcggctc ttctcgcggc ccgatggcac cagcccgctc atcggcttca
     2281 actggagcaa ctcggtcaac aacgagacga tggagatcac ggctgaattc cgcaaggcgt
     2341 tggacttcga gcacatcgtc cgcttcgagc atcacatcgt cgagacgtgg aagagcatcg
     2401 tgcggcagcc ttacgaccgc cgggacgagc tgatcgagat cgccgggcat gttgccaaca
     2461 tctccgccaa gcacgaaggc ggcgatccgg aagtcgagcg tacgcgcaag cacccgtcgg
     2521 acatcctgga ctacttccgc gagaaggccg aggtgctgca gagcggcgag atggcgctgc
     2581 tcgagcgcaa ttacctggac aagcacgccg cactgaatca tacggcgcga gcgctcacgg
     2641 agcacggcct gagtttcgtg gcggcgcgcc atctgcatca cgtgccggca aaagaaagag
     2701 tgtctgcctg accgtgagac gatggacgaa ctggtcgtcc gccatgcgtc gcctgccctc
     2761 gtagcgtctg tcctgagcgc agccgaagga aagcggaggg ggttcgtccg tcgtcaacac
     2821 aggactgagc cttgaacgag ccgtcgacct cgcaccgtcc tggtccactg cgcgtgatcg
     2881 tgctggcagc gaccgggcaa gacgtgcgca agtgctggca ctgcgacgcg tgcaacacga
     2941 atctcgcgcc cgaccaggac gtgacgctgg agatcctgct gcagatggtc gtgatgaacg
     3001 acgaggaagc cctgaccagc cgcacgctgt ggtccgatgc cgcgctggcc tcggctccga
     3061 aggtctgcag caacggcctg gacatcgagg cggtcatgct cgccttgcgc cgtgaggcac
     3121 aacggcgcgg cctgcatccg ccgattgcca attaccaatt accaaaggac agaggagatt
     3181 gaatgaagct ggcgattact ggcaagggag gagtcggcaa gacgacgctg gcggcactcc
     3241 tggcacaggt atacgccgac gctggacgcg tggtgctggc cgcggacgcc gacccgtctc
     3301 cctgcctggc cggggcgctc ggcttcccag ccgagctgcg cgcccggctg cgcccgattg
     3361 ccgagatgga cgacttgatc gaggagcgca ccggcgcgaa gccgggaacg acgggcggct
     3421 ttttcaagat caatccgcgc gtcgacgaca tccccgatcg attcagcgtg agccatcgcg
     3481 gcgtgcgact gctggagatg ggcgcggtcg acctgggcgg atcaggctgc atctgcccgg
     3541 agagcgcgat gctcaagaca ctcttcacac acttgctctt ccgcaaggac gaggtgctga
     3601 tcctggatat gtacgcgggc gtcgagcacc tgggccgcgc gacggtcgac ttcgtcgacg
     3661 cgatgctcgt cgtcgtcgag ccgacgcggc gcagcctgag tacggcggcg cagatcaaag
     3721 agctggcatc gcacatcggc ctgacgcggc tgtggctggt gggcaacaag gtgcggacga
     3781 ctgaagatcg cgccttcgtc gaggcgaact cgcccggcct gcccgtgctg ggccatctgc
     3841 cctacacgcc gctggctcag gaagccgacc gggcaggcgc ggccgcgtat gatatgatcc
     3901 cggagatagt ggaggcggcg cggtcgatcg ccgctacgct ggagaaggaa ctcgtcgctc
     3961 aggcggcgtc cgccacaggc cagccatgag tcagaagcga gacggataca cgactgagcc
     4021 ttacggactg actgaaacac accctattga ggaggctgtg catgcgtgac ctgacccaag
     4081 agttgatcga actcatccgc cgcacgtcga ccgacctgcc gccggacgtc gagaag
//
LOCUS       DBNHXB010000283        11397 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3093752, whole genome shotgun sequence.
ACCESSION   DBNHXB010000283 DBNHXB010000000
VERSION     DBNHXB010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11397)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11397)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11397
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3093752"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1838)
                     /locus_tag="AC162G_11450"
     CDS             complement(<1..1838)
                     /locus_tag="AC162G_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HIR5076479.1"
                     /translation="MKRKVYLEDIPLDAAWRRFIATLEAADLWRPFDGEEIPVADALG
                     RVTAEPIWARVSSPAYHAAAMDGYAVRSEDTVGATETAPVRLAIVDGGGDSSRRLQAQ
                     YVDTGDPLPAWADAVIPIEHVHLIHQPASEPTTLAPDQATTRPVDQATIRPPDQATTR
                     LIYIEILASVAPWSNVRSMGEDMVATELALPANHTLRPVDLGAIAGCGYASLKVRRRP
                     RVAIIPTGTELITAEQAARAGVKPGDIIEYNSIVLAAEVEQWGGLPTRYPIVPDDYDL
                     IKAAVLEAAPEHDLVLVNAGSSAGSEDYTARIVEELGTLLVHGVAVRPGHPVILGTIQ
                     SPISKSQTPNSKLQSLTPVIGIPGYPVSAALTGEIFVEPLLAKWRGQPPLRKPTMRAT
                     ITRKLLSPMGEDEWVRVTVGRVGDRVIAAPLRRGAGVITSLARADGIVRIPRFSEGVN
                     AGDEVTVELYTTPDEIERTIVHIGSHDLTLDLLAQFLAEKSGRRFVSANAGSLGGLIA
                     LRRGEAHLAGSHLLDPETGEYNISYVKQYLPGTPVSLVTLLRREQGLIVTKGNPKRIE
                     SLAHLARPDVRFVNRQRGAGTRVLLDYRLGQLGIAPEQVRGYEREE"
     gene            complement(1906..2346)
                     /locus_tag="AC162G_11455"
     CDS             complement(1906..2346)
                     /locus_tag="AC162G_11455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR5076480.1"
                     /translation="MTLVKDAPHRDRTYKLIGLAMEVHNELGPGHREADYHDALLIKL
                     KGAQLDFEDEPEVLITLDAGEMVKTRKPDFVVAQSVIIEVKGRSHLMTKDDQAQVIGY
                     FAALPECKVALFFNFGRSRLEYHRLFPPKKVAAFQRQKWGKATD"
     gene            complement(2440..3672)
                     /locus_tag="AC162G_11460"
     CDS             complement(2440..3672)
                     /locus_tag="AC162G_11460"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR5076481.1"
                     /translation="MPELFTVLTPSAAIQKLLDHLPPSAGADVVPLHEALDRVIAESP
                     VAPGALPAFPRSAMDGYAVRAADTFGASESLPAYLTVAGESPMGQAPAFEIDKGQAAI
                     IHTGGMLPKGADGVVMIERTQKARENEIEVLRAIAVGENVIQIGEDVKAGEALLPAGH
                     RLRPQDLGGLAAAGITQVRVARRPRVAILATGDEVVPADKEPAPGQVRDVNTYSIAGL
                     VKRAGGAPMPCGIVPDDFDALYHAARSALDGSDALVLSAGSSVSARDMTSRVIDRLGK
                     PGVLVHGVSIKPGKPTVLAVCDGKPVFGLPGNPVSALIVADLFLTPALWRMQGLAQPA
                     IYRSVHARLTRNVASTPGREDYIQVKLVERGGELWAEPVLGKSNLIFTLVKADGLMVV
                     PLDANGVQEGELVEVRVF"
     gene            complement(3676..4752)
                     /locus_tag="AC162G_11465"
     CDS             complement(3676..4752)
                     /locus_tag="AC162G_11465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5076482.1"
                     /translation="MGTQRFRDRFPDLPDHFRHQQGWWLSSIGIGTYLGSPDDNADAA
                     YVAAVQRAVECGINVIDSAINYRCQRSEQSIGRALKLLFESGRARRDEVVICTKGGYV
                     PYDGSPPDDPQRFIHEKYIDTAIARPEDFVDGHCMSPEFLWHQIEQSRHNLGVECIDV
                     YYLHNPETQLEKIPRAEFRTRLRAAFEFLEERVDQGLIRFYGTATWNGYRVSPQAGGY
                     LSLDDVLAVAREVRGINHHCRFIQLPCNLALLEALTLRNQRAGGEQMSVLEAAKRLGV
                     TVICSASLLHSRLLGRIPGALQAQFEGLSADAQRAIQFARSAPGVSSALVGMSRPEHV
                     DENIATARIAPLDATAFKRLFAQR"
     gene            complement(4807..5712)
                     /locus_tag="AC162G_11470"
     CDS             complement(4807..5712)
                     /locus_tag="AC162G_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HIR5076483.1"
                     /translation="MFVHLWSFALLTRPVFLLGGALLYALGAAIATSAGVPIDWRRYL
                     LGQAIVTSVQLTTHYANEYFDFEADQLIGANRTWFTGGSGVLPGGLLPPSAALGAARV
                     CAMIALVAIVAATPIEPAMGVIGVLALLGGWFYSAPPARLVASGFGELTTALIVAFLA
                     PLSGLVMQRGPVDARLIAITLPLVLMQMAMLVVVELPDLDADARAGKRTLAVRTGRAR
                     AALLHSVLLIAAFAATWIAAATGWIDSRIPLWTLLVAPLAAWQFAGVLRHGYRGWRAD
                     GVLAAGGVGLFAATAAATFAGLVTG"
     gene            complement(5731..6207)
                     /locus_tag="AC162G_11475"
     CDS             complement(5731..6207)
                     /locus_tag="AC162G_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5076484.1"
                     /translation="MSERKEAIRRHLEATRAQFIDAVAGLSEAEWEHTVASSEGAWTV
                     KQVVAHLASAEPGQTATGKRMLAGEARFPETFSLDFWNQRQVEKRKDRAPADLLSDLS
                     ESRQQLTTWIDGLSEADLDKSGQHARGDFITVAQLCFRIGEHEAQHADEIRRALGK"
     gene            complement(6249..6731)
                     /locus_tag="AC162G_11480"
     CDS             complement(6249..6731)
                     /locus_tag="AC162G_11480"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5076485.1"
                     /translation="MTTQGERDLIRKRLRDEGDKLIKTFESLSPAQRSMTIYSDGMTW
                     TLQDLVAHLGSAERAFLYYGRDILNGGEGAPEGFDINAFNKGEVGNLRVRTYEQLLDD
                     LRAVRQETIDMVSRIADEDFARTGRHPFFGQMTIEEMFKLIYRHNMMHLRDVRRAIGG
                     "
     gene            complement(6728..7351)
                     /locus_tag="AC162G_11485"
     CDS             complement(6728..7351)
                     /locus_tag="AC162G_11485"
                     /inference="COORDINATES: protein motif:HMM:NF022747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HIR5076486.1"
                     /translation="MAEKAVTKKEMRRQRRQEEKSRRAARARRERLFIWGGVALAVVV
                     AGFFAFRALNPTGQPGTAVLTQGNEHVDTLDQTHVPYNTVPPTSGPHLGTLAPWGIST
                     EPIPDELQVHNLEDGGVLIQYDCPDQGCPELEAQLTDFVTQLRVDPKYTHLILAPHAG
                     IRQASGGKPIALTAWTRYLYLDDFDRDKLIEFIDAYINIDHHVGGIG"
     gene            complement(7389..8165)
                     /locus_tag="AC162G_11490"
     CDS             complement(7389..8165)
                     /locus_tag="AC162G_11490"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5076487.1"
                     /translation="MEIRNRVALVTGGAHRVGKSIALALAAAGAHTVITYLHAVDEAA
                     RTRREIEAHGVQGLALQLDQREPAQIAALLGAVRERFSRLDILVNSAAIMERTSIFDI
                     TPEDWDRTLDTNLRGPFFMAQAAARMMLENKPGVDPVIESETSRDAAGCIVNISDLSA
                     LRPWPSYIAHSVSKAGLVALTRALAVALAPSVRVNAIAPGAVFKPPGWSDERWSRLSR
                     SAPLGRSGTPEDVAQAVLYLTRSEFITGQLLVLDGGRELR"
     gene            complement(8213..9136)
                     /locus_tag="AC162G_11495"
     CDS             complement(8213..9136)
                     /locus_tag="AC162G_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR5076488.1"
                     /translation="MRAFFIWMSRSELFRRLITGFLFVRRAARRFVAGETIDDAIAAI
                     RTLNASGTLASVDHLGENVESEADARRATEDYLAVLDRIGIECVKSHGSIKLTQLGLD
                     IGVDFCQENVARILRRAKEIGTFIRIDMESSEYTSRTLDVYRSLRREYENLGIVIQSY
                     LYRSKDDVAALCQEGANVRLCKGAYKEPPDKAFPHKRDVDARYVALARMLLSDDARAQ
                     GAYTAIATHDPKMIDAAKTYIAEHCIPRDRFEFQMLYGIRRDLQQQLVREGFTVRVYV
                     PYGAEWYPYFMRRLGERPANVWFVLRNLLRE"
     gene            complement(9146..10189)
                     /locus_tag="AC162G_11500"
     CDS             complement(9146..10189)
                     /locus_tag="AC162G_11500"
                     /inference="COORDINATES: protein motif:HMM:NF037020.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR5076489.1"
                     /translation="MVELHKPIVRAPDFAPGAWINSAPLHIDALRGRVVLADIWDFTC
                     INCIRTLPYLVEWQRRYAGFGLTIVGVHSPEFEFAKDKRQVEAAARRFGLGYPIVLDN
                     DYQTWTAYANRYWPAHYLIDRNGYIRYSQFGEGGYRELELAIQALLHDADPSVDLPEP
                     MPLLRPEDQPGAVCYRATPELYTGSDRGSLGNAEGYRPGAVMFYVEPEHRADGFFYVT
                     GPWRAARDHLAAAGESSTLILPYRAATVNAVMSPSGDPVELMLGIADPSATPLPTVDV
                     LIAQNGQPLPALEAGDDVFLDDRGRSVVRVDAPRMYNLVKNPGFEQSELRLMPQRAGF
                     AIYAFTFTSCIAE"
     gene            complement(10225..>11397)
                     /locus_tag="AC162G_11505"
     CDS             complement(10225..>11397)
                     /locus_tag="AC162G_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HIR5076490.1"
                     /translation="EEGRVRIPRDPADDYSDEIVRVRQAFVEQFTGVKLHHVSHYSFD
                     PHVAKGNCEHFTGVSQVPLGFAGPIQIDGEHAQGQFLIPLATSEGTLVASYNRGIKLL
                     NLCGGVKVTVVGDAMQRAPVFVLKDARAGREFVEWVQAHIDTIREEAEATSHVAKLTY
                     IDPYVANKFVYLRFNFTTGDAAGQNMVGRATFAACSWILDRFDGIEHFYLESNFATDK
                     KASQINIMRTRGKRVTAEAVVKRDVLVQHMRVEPESLSYAGGVANVGAFLSGANNNGL
                     HSANAITAMFIATGQDVANVAESSAGIIYSELTPEGNLYISLTIPSLIVATYGGGTGL
                     PTQRECLEMLGAYGKGKVNKLAEIVAAVALAGEISLASAISSSDWVSSHEKYGRNR"
BASE COUNT         2007 a         3573 c         3732 g         2085 t
ORIGIN      
        1 tactcctcgc gctcgtaacc ccgtacctgc tccggcgcaa tacccaactg gcccagtcga
       61 taatcgagca gcacgcgcgt gcccgcgccg cgctgccggt tcacgaaacg cacgtcgggc
      121 cgcgccaggt gcgccagcga ttcgattcgt ttcgggttgc ccttcgtcac gatcagaccc
      181 tgctcgcggc gcagcagcgt caccagggag accggcgtgc cgggcagata ctgcttaacg
      241 tacgagatat tgtactcgcc ggtttcagga tcgagcaggt gcgatccggc caggtgcgcc
      301 tcgccccggc gcagggcgat cagtccgccc aggctcccgg cattggccga gacgaatcgc
      361 cgccctgact tctctgcaag gaattgggcc agcagatcga gtgtaaggtc atgcgagccg
      421 atgtggacga tcgtgcgctc gatttcgtca ggggtggtat acagctcgac cgtgacctcg
      481 tcgcccgcgt tcaccccttc gctgaagcgc ggtatgcgca cgatgccgtc ggcgcgggca
      541 agcgacgtga tcacgcccgc gccccgtctc agcggcgcgg cgatgacacg atcgcccacg
      601 cggccgaccg tcacgcgcac ccattcgtcc tctcccatcg gcgatagcaa ctttcgggtg
      661 atcgtcgccc gcatggtggg cttgcgcagt ggaggctgcc cgcgccactt cgccagcagc
      721 ggctcgacga agatctcgcc ggtgagcgcg gcgctgaccg ggtagccggg gatgccgatg
      781 accggagtta gagattggag tttggagttt ggagtttggg atttggagat tggagattgg
      841 attgtgccga gtatgacggg gtggccgggg cggacggcga cgccgtgcac gaggagtgtg
      901 ccgagctctt cgacgatacg cgccgtgtag tcctcgctgc ctgccgatga gccggcattg
      961 accaggacca gatcgtgctc tggcgctgct tcgagcacgg cggctttgat gaggtcgtag
     1021 tcgtcgggaa cgatcgggta gcgggtcgga agcccacccc attgctcgac ctccgccgcc
     1081 aggacgattg agttgtattc gatgatgtcg ccgggcttca cgcctgcgcg tgcggcctgt
     1141 tcggccgtaa tcagctcggt gccggttgga atgatcgcca cgcggggccg gcgccggacc
     1201 ttgagcgaag catagccaca gccggcaatc gcgccgagat cgacgggccg cagcgtgtga
     1261 ttggcaggca gggcaagctc ggtggcgacc atgtcttcgc ccatcgagcg gacgttgctc
     1321 cacggcgcga cggaggcgag gatttcgatg tagattagtc gagtagtcgc ttggtcaggt
     1381 ggtcgaatgg tcgcttggtc aactggccga gtggtcgctt ggtcaggtgc tagagtggtt
     1441 ggttcgctgg ctggctggtg gatgaggtgg acgtgttcga tggggattac ggcgtcggcc
     1501 caggcgggta gggggtcgcc ggtgtcgacg tattgtgcct gtaatcggcg actagagtcg
     1561 cctccaccat cgacgattgc tagccgtacg ggtgcggttt ccgtcgcgcc gaccgtgtct
     1621 tcgctgcgaa ctgcataacc gtccatcgcc gccgcgtggt aggcgggcga cgaaacgcgc
     1681 gcccagatcg gctcggcagt gacgcggccc aaggcgtcgg cgacgggaat ctcttcgccg
     1741 tcgaagggac gccataagtc ggcggcctcc aacgtggcga tgaatcgccg ccaggccgcg
     1801 tcgagcggga tgtcctcgag gtagactttg cgtttcatgt caaatgcggg gcaacgaatg
     1861 tacaacggat aaacgaatcg cacagcgtta tccgtttatt cgttgctaat ccgttgcctt
     1921 gccccacttc tggcgctgaa aggcggccac ctttttaggt ggaaacaggc gatgatactc
     1981 cagccgggac cgaccgaagt tgaaaaacaa cgccactttg cattcaggca gagcggcgaa
     2041 gtaaccaatg acttgcgctt gatcgtcttt ggtcatcagg tgcgagcggc ctttgacctc
     2101 gatgatgaca gactgagcga cgacaaaatc gggcttgcgg gttttgacca tttcgccagc
     2161 gtcgagcgtg atcagtacct cgggttcgtc ttcaaagtcg agttgtgccc ccttgagctt
     2221 gatcaagagc gcgtcgtggt agtcggcctc gcgatgaccg gggccaagct cgttatgaac
     2281 ttccatcgcc aggccgatga gtttgtacgt gcggtctctg tggggagcgt ctttgaccag
     2341 agtcatgtga cttctccaga aatcgatgca tcggatgtgc aacgtataaa cggattaccg
     2401 tggcgcctca catccattta tccgttgtct attcgttgct cagaacaccc tcacctcaac
     2461 caactcccct tcctgcaccc cattcgcatc cagcggcacg accatcaacc cgtcggcctt
     2521 caccagcgtg aaaatcagat tgctcttgcc taagactggc tcggcccata gctcaccgcc
     2581 gcgttccacg agcttcactt gaatatagtc ctcgcgcccc ggcgtcgagg cgacgttgcg
     2641 cgtgagcctc gcatgaaccg agcgatagat cgcgggttgc gccagccctt gcatgcgcca
     2701 cagcgccggc gtgaggaaca aatcggcgac gatcagcgcg ctgaccgggt tgccgggcag
     2761 accgaacacg ggcttgccgt cgcacacggc gaggaccgtc ggcttgcccg gtttgatcga
     2821 cacgccgtga accagcacgc cgggtttgcc gagccggtcg atcacccgag aagtcatgtc
     2881 gcgcgcactc accgacgatc cggccgacaa caccagcgcg tcgctgccgt cgagcgcaga
     2941 gcgggccgca tggtatagcg cgtcgaagtc gtcggggacg ataccgcatg gcatcggcgc
     3001 gccccctgcg cgcttcacca gcccggcgat gctgtatgta ttcacgtcgc gcacctggcc
     3061 gggcgcaggc tctttgtcgg cgggaaccac ctcatcacca gtggccagaa tcgctacgcg
     3121 cggtcgtctg gcgacgcgca cctgggtgat accggctgcc gccaggcctc ccaggtcctg
     3181 cggacgcagg cggtggccgg ccggcagcag agcctcgccg gctttgacgt cttcaccgat
     3241 ttgaatgacg ttctcgccga cggcgatcgc ccgcagcact tcgatctcgt tctcgcgcgc
     3301 cttttgcgtg cgctcgatca tcacgacgcc gtccgcgcct ttgggcagca tgccgccagt
     3361 gtgaatgatg gcggcctgac ccttgtcgat ctcgaacgcc ggtgcttggc ccatgggtga
     3421 ctcaccggcg acggtcaggt aggcggggag cgattcactc gcgccgaacg tgtctgccgc
     3481 gcgcacggcg tacccgtcca tcgcgctgcg cgggaaggcc ggcagcgctc cgggcgcgac
     3541 aggcgattcg gcgatgacgc ggtcgagcgc ttcgtggagc gggaccacgt cggcgccggc
     3601 gctaggcggc aagtggtcga ggagcttctg aatggcggct gacggggtga ggacagtgaa
     3661 cagctcgggc atggatcatc gctgcgcgaa gaggcgcttg aaggcagtcg cgtcgagcgg
     3721 cgcgatgcgc gccgtggcga tgttttcgtc gacgtgctcc gggcggctca tgcctacgag
     3781 ggcgctggac acgccgggcg ccgagcgtgc gaactggatc gcgcgctgcg cgtcggccga
     3841 caatccctcg aattgcgcct gaagcgcgcc ggggattcgg cccaacaggc gcgagtggag
     3901 cagcgatgcg ctgcagatca cggtgacgcc gagacgcttg gcggcttcta gcacgctcat
     3961 ttgctccccg cccgcgcgtt ggttgcgcag cgtcaacgcc tcgagcagcg ccaggttgca
     4021 cggcaattgg atgaagcggc aatgatggtt gatgccgcgc acctctcgcg cgacggccag
     4081 cacgtcgtcg agcgacagat acccaccggc ctggggcgac acgcgatagc cgttccacgt
     4141 ggccgttccg tagaaccgga tcaagccctg atcgacgcgc tcctctagaa actcgaacgc
     4201 cgcgcgcagc cgggtccgga attcggcgcg cggaatcttt tcgagctgcg tttcgggatt
     4261 gtgaaggtag tagacgtcga tgcactcgac gccgaggtta tgtcgcgact gctcgatctg
     4321 gtgccacagg aattcgggtg acatacagtg cccgtcaacg aaatcttcgg gccgtgcgat
     4381 agccgtgtcg atgtacttct cgtggatgaa gcgctgcggg tcgtcaggag ggctgccgtc
     4441 gtacgggacg tatccgccct ttgtgcagat gacgacctcg tcgcgccgcg cccggcctga
     4501 ctcgaacagc agcttgagcg ctcggccgat gctttgctcg ctgcgctggc agcgatagtt
     4561 gatcgcacta tcgatcacgt tgatgccgca ctcgaccgcg cgctgcacgg ctgccacgta
     4621 agcggcgtcg gcgttatcgt ccgggctgcc gaggtacgtg ccaatgccga tggacgaaag
     4681 ccaccagccc tgctggtgcc ggaaatggtc cggcaggtca gggaaccggt cgcggaagcg
     4741 ctgtgtgccc accgttgtgg caccgggtcc ggggcggaag tctgtgtgca taccggtcag
     4801 aggaacctag ccggtgacga gtccggcgaa cgtcgccgcc gccgtcgcgg cgaagagtcc
     4861 tacgccgccc gcggccagaa cgccatcggc gcgccatccg cgatagccat gccgcaagac
     4921 gccagcgaac tgccatgcag ccaacggcgc aacaagcagc gtccagagcg ggatgcgtga
     4981 atcgatccag cctgtcgccg ccgcgatcca ggtcgcggca aatgccgcga tcaacaacac
     5041 gctgtgtaag agcgcggcgc gcgctcgacc ggtccgcacc gcgagcgtgc gcttgcccgc
     5101 gcgagcgtcg gcatcgaggt cgggaagctc gacgacgacg agcatcgcca tctgcatcag
     5161 cacgagcggc agggtaatgg cgatgagacg cgcgtcgacc gggccgcgct gcatgacgag
     5221 tccgctcaac ggcgccagga acgcgacgat cagcgccgtc gtcagctcgc cgaatccgct
     5281 ggcgacgagg cgggcaggcg gagcagagta gaaccagccg cccagcagcg ccagtacgcc
     5341 gatcacgccc atggcaggct cgatcggtgt ggccgcaacg atggcgacga gggcgatcat
     5401 cgcacacaca cgcgcggcgc cgagcgccgc gctcggcggg agcaagcctc cgggaagcac
     5461 gccgctgcca cctgtgaacc acgtgcggtt tgcgccgatg agctggtcgg cttcgaagtc
     5521 aaagtactcg ttggcgtaat gcgtcgtcaa ctgcacgctg gtgacgatcg cctggccgag
     5581 caggtagcgg cgccaatcga tcgggacgcc ggcgcttgtc gcgatcgccg cacccagcgc
     5641 gtacaacagc gccccgccga gcagaaacac agggcgcgtc aataaggcga aagaccacag
     5701 atggacgaac atgggcggct cgtgtgtggg ctattttccc agcgcgcgtc tgatctcatc
     5761 ggcgtgctga gcctcatgct cgcctatgcg gaagcacaac tgcgcgacgg tgatgaagtc
     5821 gccgcgtgcg tgctggccag acttgtccag gtcagcctcg ctcagtccgt cgatccaggt
     5881 ggttaactgc tggcgcgatt cgctcaggtc gctcaacaga tcggcaggag cccgatcctt
     5941 gcgcttttcg acctgccgct gattccagaa gtcgaggctg aaggtttccg ggaaccgggc
     6001 ttcgccggcg agcatgcgct tgccggtggc tgtctggcca ggctcggcag atgccaggtg
     6061 cgccacgacc tgcttgaccg tccacgcgcc ttcgctcgac gcgacggtgt gctcccactc
     6121 cgcctcggat agtccggcga cggcgtcgat gaactgggcg cgcgtcgctt cgagatgccg
     6181 gcgtatcgcc tctttgcgct cactcattcg aatctccccc gatggcgggg cgcacgcaag
     6241 gcgcgcccct accccccgat ggcgcggcgc acgtcgcgca ggtgcatcat gttgtgacga
     6301 tagatgagct tgaacatctc ctcgatcgtc atctggccga agaaggggtg gcgacccgtg
     6361 cgcgcgaagt cttcgtcggc gatgcggctg accatgtcga tcgtctcctg gcggacggcg
     6421 cgcaggtcgt cgagcaactg ctcgtaggtg cggacgcgca gattgccgac ttcgcccttg
     6481 ttgaaggcat tgatgtcgaa gccttcaggc gcgccttcgc cgccgttgag gatatcgcgt
     6541 ccataataca gaaaagcccg ctccgcagac cccaggtgcg caaccaagtc ctgaagcgtc
     6601 caggtcattc cgtcggaata aatggtcatc gatcgctgag cgggcgacag gctttcgaac
     6661 gtcttgatca gcttgtcgcc ctcgtcgcgc agccgctttc gaatcaggtc gcgctcgccc
     6721 tgcgtcgtca cccgatccct cccacatggt gatcgatgtt gatgtaagcg tcgatgaact
     6781 ctattaattt atcacgatcg aagtcgtcga gatacagata gcgcgtccag gcggtgagcg
     6841 cgatcggctt tccaccgctg gcctgtcgaa tgcccgcatg aggagccagg atgaggtgtg
     6901 tgtatttggg atcgacccgc aattgggtga cgaagtccgt caactgggct tccagctcgg
     6961 gacaaccctg gtcgggacag tcatactgaa tgagcacgcc gccgtcctcc aggttgtgga
     7021 cctgaagctc atcgggaatc ggctcggtcg agatgcccca cggcgccagc gtgcccaggt
     7081 gcgggccgga agtcggtggc acggtattgt agggcacgtg cgtctggtca agtgtatcta
     7141 catgctcgtt gccctgagtc agcacggccg ttcccggctg gccggtcgga ttgagcgccc
     7201 gaaacgcgaa gaaaccggcc acgacgacgg caagggccac tccaccccag atgaacaggc
     7261 gctcccgtcg ggcgcgtgcc gcccgtcgag atttctcctc ctggcgccgc tggcggcgca
     7321 tctccttctt ggtgacagcc ttctcagcca atgcctaact cctccttgaa atgtaagtgc
     7381 cactaagttt accttaactc gcgtcccccg tccaacacca ggagctggcc ggtgatgaat
     7441 tcgctgcggg tgaggtagag gaccgcctgc gcgacgtcct ccggcgtgcc ggaccggccg
     7501 agcggggcac tccgcgataa tcgagaccac cgttcgtcgc tccatcccgg cggcttgaac
     7561 actgctcccg gcgcgatcgc gttgacacgg actgacggcg ccagcgccac cgccaatgcc
     7621 ctggtcagcg cgacgagtcc tgccttgctg acactgtgcg cgatatacga cggccaaggc
     7681 cgcagcgcgg acaggtcgga aatgttgacg atgcaccccg ctgcatcgcg cgacgtctcg
     7741 ctctcgatga cagggtccac gccgggtttg ttttccagca tcatgcgcgc agcggcttgt
     7801 gccatgaaga agggaccgcg caggttggtg tccagcgtgc gatcccagtc ttcgggcgtg
     7861 atgtcgaaga tcgacgtgcg ctccatgatc gccgcgctgt tgacgaggat gtcgaggcgg
     7921 ctgaaacgct ctcgcacggc gccgagcagc gccgcaatct gcgccggctc gcgctggtcg
     7981 agctgcagcg ccagcccttg cacaccgtgc gcttcgatct cgcggcgcgt gcgagccgcc
     8041 tcgtcgacag cgtggagata ggtgatgacg gtgtgcgcgc ctgcggccgc cagcgccagc
     8101 gcgatcgact tgccgacgcg gtgtgccccg ccggtcacaa gcgcgacccg gttgcgaatc
     8161 tccaatctcc aatctcctcg gacctgacag gtttctgaaa cccgtcaggt ctttattcac
     8221 gaagcaggtt gcgcagcacg aaccacacgt tggccggtcg ctcgcccagg cgacgcatga
     8281 agtacggata ccattcggcg ccgtaaggca cgtacacgcg cactgtgaag ccttctcgga
     8341 cgagctgctg ctgcaggtcg cggcggatgc cgtacagcat ctggaactcg aaccggtcgc
     8401 gcgggatgca atgctccgcg atgtacgttt tcgcagcgtc gatcatcttc ggatcgtgcg
     8461 tcgcaatggc ggtgtaagcg ccctgcgcgc gggcgtcgtc actcagcagc atgcgcgcca
     8521 gtgcgacgta tctggcgtcg acatcgcgct tgtgtgggaa cgccttatcg ggcggctcct
     8581 tgtaagcgcc tttgcacagc cggacgttgg cgccctcctg gcacagcgcg gcgacgtcgt
     8641 ccttgctgcg gtacaggtac gattgaatga cgataccgag attctcgtat tcccgacgca
     8701 ggctgcgata gacgtcgagc gtgcgagagg tgtattcgct cgactccatg tcgatgcgaa
     8761 tgaacgtgcc gatttccttc gctcggcgca ggatacgggc gacgttttcc tggcaaaagt
     8821 cgacgccgat atccaggccc agttgcgtga gcttaatcga cccgtgcgac ttcacgcatt
     8881 ctatgccgat tcgatccagc acggccaggt agtcttcagt ggcgcggcgt gcgtcggcct
     8941 cactctcgac gttctcaccc aggtgatcga ccgaagccag agtgccgctg gcgttcaacg
     9001 tgcggatggc ggcgatggcg tcgtcaatcg tttcgccggc gacgaagcgc cgtgcggcgc
     9061 ggcggacgaa gaggaagccg gtgatgaggc ggcggaacaa ctcgctgcgg gacatccaga
     9121 tgaaaaaggc gcgcatgggg tgtcctcatt cggcgatgca agatgtgaag gtgaacgcgt
     9181 atatggcaaa cccggcgcgc tgtggcatca accgcagctc agactgctcg aacccggggt
     9241 ttttgacgag attgtacatg cgcggcgcgt cgacgcggac gacgctgcga ccacgatcgt
     9301 caaggaacac gtcgtcgcca gcttcgagcg ccggcagcgg ctgcccattt tgcgcgatca
     9361 acacgtcgac ggtgggcagg ggcgtcgcgc tcggatcggc gatgcccagc atcagctcga
     9421 cgggatcacc gctcggcgac atcacggcgt tgacggtggc cgcccgatac ggcaggatca
     9481 gcgtgctgct ctcaccggct gcggccagat gatcgcgcgc cgcgcgccac ggcccggtca
     9541 catagaagaa accgtcggcg cgatgctccg gctcaacgta aaacatgact gcgccgggcc
     9601 ggtagccttc ggcattgccc agcgatccgc gatcagaacc tgtgtaaagt tccggcgtcg
     9661 cgcggtaaca gacggccccc ggctggtctt cgggccggag cagcggcatc ggctcaggca
     9721 gatcgacgga cgggtcggca tcgtgcagga gcgcctggat cgctaactca agctcgcgat
     9781 agcctccttc gccgaactgg ctgtagcgga tgtaaccgtt cctgtcgatc aagtaatgcg
     9841 cgggccagta acgattcgcg taagcggtcc aggtttggta gtcgttgtcg agcacgatgg
     9901 gataccccag accgaaacgg cgcgcggcgg cctcgacctg acgcttatct ttcgcgaatt
     9961 caaattccgg cgagtgcacc ccgacgatgg tcagaccgaa gccggcgtag cggcgctgcc
    10021 actcgaccag gtagggcagc gtgcggatgc agttgatgca ggtgaaatcc cagatgtcgg
    10081 caaggacgac gcgaccgcgc agcgcgtcga tgtggagggg cgcggagttg atccaggcgc
    10141 cgggagcgaa gtcgggcgcg cggacgatcg gcttgtgtag ttcgaccatg ccgcgattct
    10201 accacgaaga aatgatgagt agatttagcg gttgcggccg tatttctcgt ggctcgaaac
    10261 ccagtccgac gacgagatcg cagaggccag ggatatctcg cccgccagcg ccacggcggc
    10321 cacgatctcc gcgagcttgt tgaccttgcc tttgccgtaa gcgcccaaca tttcgagaca
    10381 ctcgcgctgg gtcggcaggc cggtgccgcc gccgtacgtc gccacgatca gggaagggat
    10441 cgtgagtgaa atgtacaggt tgccttctgg cgtcaactcg gagtaaatga tgcccgccga
    10501 cgattcggcg acgttggcga cgtcctgccc cgtggcgatg aacatggcgg tgatggcgtt
    10561 cgccgaatgc agcccattgt tgttggcgcc ggacagaaac gccccgacgt tcgcgacgcc
    10621 gccggcataa gacaggctct ccggctcgac gcgcatgtgc tggacgagca cgtcgcgctt
    10681 gacgacggct tcggcggtga cgcgcttgcc gcgcgtgcgc ataatattga tctgcgaggc
    10741 tttcttgtcc gtggcgaagt tggactcgag gtagaagtgc tcgatgccgt cgaaacggtc
    10801 caggatccaa ctgcacgccg cgaacgtggc gcggcccacc atgttctgcc cggcggcgtc
    10861 gcccgtcgtg aagttgaagc gcaggtagac gaacttgttg gcgacgtacg ggtcgatgta
    10921 ggtgagcttc gcgacgtgcg acgtggcctc cgcttcttcg cgaatcgtgt cgatgtgcgc
    10981 ctgcacccat tctacgaact cgcgcccggc gcgagcatct ttcaagacga agaccggcgc
    11041 gcgctgcatc gcgtctccga cgacggtcac cttcacgccc ccgcatagat tgaggagctt
    11101 gatgccccgg ttatacgagg cgacgagcgt gccctcggaa gtcgccagcg ggatcaggaa
    11161 ctggccctgc gcgtgttcgc cgtcgatctg aataggcccg gcaaacccca gcggcacctg
    11221 cgacacaccg gtgaagtgct cgcagttgcc tttcgcgacg tgtggatcga atgagtaatg
    11281 gctgacgtgg tgaagcttca cccccgtaaa ttgctcgacg aaagcctgcc ggacgcgcac
    11341 gatctcgtcg gaatagtcgt cggccggatc gcgcgggatg cgcacgcgcc cttcctc
//
LOCUS       DBNHXB010000284         6406 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3262155, whole genome shotgun sequence.
ACCESSION   DBNHXB010000284 DBNHXB010000000
VERSION     DBNHXB010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6406)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3262155"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="AC162G_11510"
     CDS             <1..285
                     /locus_tag="AC162G_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076491.1"
                     /translation="GDRLMPTVPIPQVEVGDVLALLDTGAYQEVSMSNFNAMPRPATV
                     LVTGDQATVIRRAETETDVFRRDVVPPHLRSIQVAETEDVRARSSVPSRP"
     gene            complement(299..2323)
                     /locus_tag="AC162G_11515"
     CDS             complement(299..2323)
                     /locus_tag="AC162G_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012377.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HIR5076492.1"
                     /translation="MHTGIAIVPVGRVETRILARVCAVVTETFGRPCRIEDALPAPEY
                     AFDPRRRQYAAEANTAFKDEHGRTLILRGVNLGGSSKVPFAPNGATHLRDGFFDHRRV
                     SFVGRPFPLDQADEHFTRLREWGFTFLRLLITWEAIEHAGPGEYDEAYLDYLWAVVKK
                     AGDYGFGVCIDPHQDMWSRFSGGDGAPGWTLDAVGLDLTQFAETGAAITHQTHGDPFP
                     HLIWPSNETRLATATMFTLFFGGDDFAPATRIEGESAQSYLQRHYVAAVARAAHRLKD
                     LPNIVGYDTLNEPSPGYIGWPDLRAAGGQVKKGPCPSPYQAMLLGAGFPQTVAVWDMR
                     LSGFKRIGARALNTSRARAWQPGHDCVWRQNGVWDVGLDGSPHLLRPEHFAQRAGRAV
                     DFSQDYYLPFARRFAEAIRAADRRAILFVESAPRQPPPRWNMPCLAYAPHWYDGYVLA
                     LKRYSRWLAVDFHTGRLVFGPGRVRRSFAAQLNRFKREAAERLNDAPVLIGELGIPFD
                     LHGGRAYHTGDFSAQIQAMDRALRALEDTLLSGALWNYTADNTNARGDQWNGEDLSIF
                     SRDQQTDPADIHSGGRALPAVVRPYARRIAGKPLRMGFEVRRRVFEFEFRHAAGVSEP
                     TEIFVPNYQYPQGCAVKVSDGTYSIDQMAQTLRYRHGLDRPTHTIRIEAL"
     gene            complement(2325..3059)
                     /locus_tag="AC162G_11520"
     CDS             complement(2325..3059)
                     /locus_tag="AC162G_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR5076493.1"
                     /translation="MTIHIGTSGWVYNHWRGIFYPERLRQSEWFACYAREFDTVEINN
                     SFYRLPGETTFDKWRTQAPPGFLYAVKASRFLTHLKKLKDPEEPLRRFFERAGRLGET
                     LGPVLYQLPPHWQVNLPRFEHFLSVLPKGYRHVVEFRDASWLTEDVFRLMERHRVAHC
                     IHDLQPLNVPLRVTTSMVYLRFHGDPSHGGNYPHAALEAWARRLAAWRSQGLGVFAYF
                     NNDIGGYALENAKTLRALLPEVHRGT"
     gene            3129..3365
                     /locus_tag="AC162G_11525"
     CDS             3129..3365
                     /locus_tag="AC162G_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076494.1"
                     /translation="MLGKLHVPQQAPSGPIQIPMVYVKGKSVWEYKQLMRNLRTEQAP
                     GQDELNALGAEGWELAGVFADLPFVYFYFKRLVE"
     gene            complement(3486..6290)
                     /locus_tag="AC162G_11530"
     CDS             complement(3486..6290)
                     /locus_tag="AC162G_11530"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5076495.1"
                     /translation="MSEPLHSLDDIARQHSLLATKLRPPHLRARLVRRPRLTAHLDEA
                     LQHRLTLISAAAGSGKTTLLSEWQDAARRQRWPIAWVSLDKGDNDPVRFWSHVTAALD
                     AFQTGISRTTLMALHATPLPDPSVMQDGPAWIEAILAVLVNELATLPRDVVVVLDDYH
                     VIESSAVHDSLAYWLEHVPDCVHLVIASREDPPMPLARLRVRGELAEIRGADLRFTRG
                     EVATYFNEVMGLHLPDEDIAAIEARTEGWIAGLQMAALSMQGRRDTAGFIAAFTGSHR
                     FVADYLIEEVLGRQPEAIQSFLLQTAILNRFNAALCDAVTSQTDSQAVLERLDAANLF
                     LVSLDDKRRWYRYHPLFAELLRDRLSRLQPDRVPDLHRRACDWHERNGLVADAIEHAI
                     AMKDWERAADLLEHASRRTWMPWTQSEIGAMLEWLEALPPDLVRARPRLCLACAWALL
                     DPSQIDRVEQYLQDATQAVNSLRGEKQVQAVLCEVAVIRTFVSSIRGDAPLTIELARQ
                     ALASLPETEVSLRSMIAWSLGYAYALTRDMPSAEQALTEALSLSRVAGNHALALMSMN
                     ELAEIQCDCGRLRESARTLQQTLELAEEWGGQNFFILSRTWWLQAWLFYEWNDLERAA
                     TCLGESHKIAAQWRYLRGVVSARGLLAIVKQAQGDSEGAQEMIRQARQAALESRVPIA
                     ADTLAVYQLNLWRARGDLESAMQWIREHELNWSDKGSRIHYAAGTAVARVLIEHSRKQ
                     NEQTWMHKAFELLEDLLQKAEAAGLALDSIDILPLQATALHVQGQTAQALRVLKRALV
                     LAEPEGYVRMFLECGEPMAQLLRWSIESKEWNEPRLEAYARRLLAQFGQPQPEAGMAA
                     SSLPVLASGDEGWLEPLTGREMEVLRLVAAGASDQEIAEKLGLAKATVKTHLRHIYRK
                     LQVGRRTQAIVRARLLRLLP"
BASE COUNT         1168 a         2012 c         2049 g         1177 t
ORIGIN      
        1 ggcgaccggc tgatgccgac cgtgccgatt ccgcaggtgg aagtgggcga tgtgctggcg
       61 ctgctggata ccggcgcgta ccaggaagtg tcgatgagca acttcaacgc gatgccgcgt
      121 ccggctaccg tgctggtgac cggcgatcag gccaccgtca ttcgtcgcgc agagacagag
      181 accgatgtct tccggcgcga cgtggtgccc ccacacttac gaagcattca ggtcgccgag
      241 acagaggatg tgagggctcg atcgagtgtc ccgtcgaggc cgtgatgccc gcctgcattc
      301 agagcgcctc gattcggatc gtgtgtgtag gccgatcgag gccgtgccgg tagcgaagcg
      361 tctgcgccat ctgatcgatg gaatacgttc cgtcggacac tttgacggcg catccttgag
      421 gatactggta gttcggcacg aagatctcgg tcggttcgga cacacccgca gcgtgccgaa
      481 actcgaattc gaatacacgg cggcgcacct cgaagcccat gcgcagcggc ttgccggcga
      541 tcctgcgcgc gtaaggccgc acgacggccg gcagagcgcg cccaccggaa tggatgtcgg
      601 ccgggtcggt ctgttgatcg cggctgaaga tcgacaggtc ttcgccgttc cactgatcgc
      661 cgcgcgcgtt cgtgttgtcg gccgtgtagt tccacagcgc gccgctcagc agcgtatcct
      721 ccagcgcgcg caaggcgcga tccatggcct gaatctgcgc gctgaagtct ccggtgtgat
      781 aggcccgacc cccgtgcagg tcgaacggga taccgagctc gccgatgagt accggcgcgt
      841 cgttcaatcg ctcggccgcc tcgcgcttga agcggttcaa ctgcgcagcg aacgacctgc
      901 ggacgcgacc cggcccgaac accagccggc cagtgtggaa gtcaaccgcc agccagcggc
      961 tgtatcgctt cagggccagc acgtagccgt cgtaccagtg cggcgcgtag gccaggcacg
     1021 gcatgttcca gcgaggcggc ggttgtcgcg gcgcgctctc gacgaacagg atggcgcgcc
     1081 gatcggcggc gcgaatggct tccgcgaatc gcctggcgaa ggggagatag tagtcctggc
     1141 tgaagtccac cgcccggccg gcgcgctgcg cgaagtgttc ggggcgcagc aggtgcggcg
     1201 atccatccag ccccacgtcc cacacgccgt tctgccgcca cacacaatcg tgtccgggct
     1261 gccacgcccg ggcgcgcgag gtgttgagcg cccgagcgcc gatacgcttg aagccggaca
     1321 atcgcatatc ccacacagcg acggtctggg ggaagcccgc gccgagcagc atggcttgat
     1381 acgggctggg gcatggcccc ttcttgacct ggccgcccgc cgcgcgcaga tcgggccagc
     1441 cgatgtagcc gggcgacggc tcgttgagcg tgtcgtagcc gacgatgttg ggtaagtcct
     1501 tcagccggtg cgcggcgcgg gccacggcgg cgacgtagtg ccgctgaaga taggactgcg
     1561 ccgattcgcc ttcgatgcgc gtggccggcg cgaagtcatc gccgccgaag aacagcgtga
     1621 acatcgtggc ggtggcgagc cgggtttcgt tcgagggcca gatcaggtgc ggaaagggat
     1681 cgccgtgcgt ctggtgcgtg atggccgcgc cggtttcggc gaactgcgtg aggtccagcc
     1741 cgaccgcgtc gagcgtccag ccgggcgcgc cgtcgccgcc tgagaagcgg ctccacatgt
     1801 cttgatgcgg atcgatgcag acgccgaatc cgtagtcgcc cgccttcttg accacggccc
     1861 acaggtagtc cagataagcc tcgtcgtact ctcccggccc ggcgtgctcg atggcttccc
     1921 acgtgatgag cagacgcagg aaagtgaagc cccattcccg caggcgcgtg aagtgctcat
     1981 cggcctgatc gagcgggaaa ggtcggccga cgaaagacac ccgtcgatga tcgaagaaac
     2041 cgtcgcggag gtgcgtcgcg ccattggggg cgaacggcac tttgctgctg ccgccaagat
     2101 tgacgccgcg cagaatcagg gtgcggccgt gttcgtcttt gaaggctgta ttcgcttcgg
     2161 cggcatactg ccggcggcgc ggatcgaagg catactcagg cgcgggcagg gcatcctcga
     2221 tgcggcacgg ccgtccgaac gtctccgtca ccactgcaca gacacgtgcc aggatccttg
     2281 tctcgaccct cccgaccggc acgatggcaa tcccggtgtg catctcacgt tcctctgtga
     2341 acctcgggta gcagcgctcg cagcgtcttt gcattctcca gggcgtagcc gccgatatcg
     2401 ttgttgaaat aggcgaagac gccaagcccc tgactgcgcc acgcggcgag gcgcctggcc
     2461 caggcttcga gcgcggcgtg cgggtagttg ccgccatgcg atgggtctcc gtggaaacgg
     2521 aggtagacca tcgatgtggt gacgcgaaga ggaacgttca gcggctgcag gtcgtgaata
     2581 cagtgtgcga cgcggtggcg ctccatcaat cgaaagacat cttcggtcag ccagcttgcg
     2641 tcgcgaaact cgaccacgtg cctgtatcct ttcggcaaga cagacagaaa gtgctcgaag
     2701 cgcggcaggt tgacctgcca gtgcggcggg agctggtaga gcaccgggcc gagcgtctcg
     2761 cccaggcgac cggcgcgctc gaagaatcgc cgcagcggct cctccgggtc tttgagtttc
     2821 ttcaggtggg tgaggaagcg gctggccttg acggcgtaca ggaaaccggg tggcgcctgc
     2881 gtgcgccact tgtcgaacgt cgtctcgccc ggcagccggt agaacgagtt gttgatctcc
     2941 acggtgtcga attcccgcgc gtagcaggcg aaccactcgc tttgccgcag gcgctccgga
     3001 tagaaaatgc cgcgccagtg gttatagacc cagcccgatg tgccgatgtg gatcgtcatc
     3061 ccgtcacctg cttgtcaccc tggttcgcca agagtatact acctgccacc gattcaggag
     3121 gtaattcaat gcttggcaag ctgcatgtcc ctcaacaggc tccgagtggg cccatccaga
     3181 tcccgatggt ctatgtgaag ggcaagtccg tctgggagta caaacaactg atgcgcaacc
     3241 tgcggacgga acaagcgccg ggccaggacg aactgaacgc gctgggcgcc gaggggtggg
     3301 aactggcggg cgtcttcgcc gatttgccgt ttgtgtactt ctacttcaag cgactggtcg
     3361 agtgagacag gtgtgatcct tgtgtgtggg agggtgaacg cgcgcatgcg gggtgtcgaa
     3421 ctggagttgc ccgacgccac tgatggcgcc gagggggaag gtgcgggatg ccaggctcgc
     3481 tggcgctacg gcagcaaccg caacagcctc gctcgaacga tcgcctgggt gcggcgaccg
     3541 acttgcagtt ttcggtagat gtgccgcaga tgtgtcttga ccgtggcttt tgccaaaccc
     3601 agtttctcag cgatttcctg atcggacgcg ccggccgcga ccagccgaag cacttccatc
     3661 tcgcgcccgg tgagcggctc cagccatccc tcgtcgccgg acgccagaac tggcagcgat
     3721 gaagccgcca tgcccgcttc cggttgaggc tgcccgaact gagcgagcag cctgcgcgca
     3781 tacgcctcca ggcgcggctc gttccactct ttgctctcaa tgctccaccg aagcaattgc
     3841 gccatcggtt cgccgcattc cagaaacatg cgaacatacc cttcgggttc tgccaacacc
     3901 aacgcgcgct tgagcactcg gagtgcctga gcagtctggc cctgcacatg tagcgctgtg
     3961 gcttgaagcg gcaagatgtc aattgaatcg agagcgagtc ccgctgcctc ggctttctgc
     4021 aataagtctt ccagtaactc gaatgccttg tgcatccagg tctgttcgtt ctgtttccga
     4081 ctatgctcga ttaagacgcg cgccacagcg gttcccgcgg cgtagtggat acggctgcct
     4141 ttgtcgctcc aattcagttc gtgctctcga atccactgca tcgcggactc cagatcgccc
     4201 cgcgctctcc agagattcaa ttgatagacc gccagcgtgt ctgcggcgat cggtacacgc
     4261 gactccagcg cggcttgcct ggcctgtcga atcatttcct gcgcgccctc gctgtcgcct
     4321 tgcgcttgtt tcacgatggc cagcaagccg cgagcactca cgacccctct caagtatctc
     4381 cactgcgccg ctatcttgtg gctttcgccg agacaggtgg ctgctctttc gaggtcgttc
     4441 cactcataga acagccaggc ttgcagccac caggtcctgc tcaagataaa gaaattctgc
     4501 ccgccccatt cttcggccag ttcgagcgtc tgctgcagcg ttctcgcgct ctcgcgcagt
     4561 ctgccgcaat cgcattgaat ttccgccagc tcattcatgg acatcaaggc cagcgcatga
     4621 ttgccggcaa cccgactcag cgatagggcc tcggtcaggg cttgttcggc cgacggcata
     4681 tccctcgtga gcgcataggc atatcccagc gaccaggcga tcatgctccg caaagacact
     4741 tcagtctctg gaaggctcgc cagcgcctga cgagccagct cgatggtgag cggcgcatca
     4801 ccgcgaatgc tggagacaaa ggtgcggatg accgccactt cgcacaagac agcctgcacc
     4861 tgtttctcac ctcgcagcga gttgacggct tgtgttgcat cctgcaagta ctgttcgacg
     4921 cgatcaatct ggctgggatc gagcaaagcc caggcacagg ccagacacaa tcgcggtcgc
     4981 gcacgaacga ggtctggcgg caacgcttcg agccactcga gcatcgcgcc gatctcgctt
     5041 tgcgtccagg gcatccatgt tctgcgagag gcgtgctcca gcagatccgc cgcccgctcc
     5101 caatccttca tcgcgatggc atgctcgatc gcgtcggcca cgagtccgtt acgctcgtgc
     5161 cagtcgcagg cgcggcggtg cagatcggga acgcgatccg gctgcaggcg gctcaagcgg
     5221 tcgcgaagca gttcggcgaa cagcggatgg tagcgatacc atcttcgctt gtcatcgaga
     5281 gacaccagga acaagttcgc cgcgtctaag cgttccagca ccgcctggct atccgtttgg
     5341 ctggtgacgg cgtcgcacag cgcagcattg aagcggttaa ggatggccgt ctgcagcaaa
     5401 aaggactgaa tagcctcagg ttgtcgtccc agcacttcct cgatcagata gtctgcgacg
     5461 aagcgatggc tgccggtaaa ggcggcgatg aatccagccg tgtcccggcg cccttgcatg
     5521 gacaacgccg ccatctgcag accggcgatc caaccttccg tccgcgcctc gatagctgca
     5581 atgtcctcgt cgggaaggtg cagccccatg acctcgttga agtaggttgc cacttcgcct
     5641 cgagtgaaac gcaagtcggc gccacgaatc tcggccagtt cgccgcgaac acgcaagcgg
     5701 gccagcggca tgggtgggtc ttcccggctt gcgatgacaa ggtgtacaca gtccggcacg
     5761 tgctcgagcc aatacgcaag ggaatcatga actgcggatg actcgatgac gtgatagtca
     5821 tccagaacaa ccacgacgtc gcggggtagg gtggccagct cgttgaccag gaccgccagg
     5881 atagcttcaa tccatgccgg gccatcttgc attacggaag ggtccggcag cggtgtcgca
     5941 tgcagagcca tcagagtcgt ccggctaatc cctgtttgga atgcgtccag cgcagcggtg
     6001 acgtgcgacc agaaacgcac cgggtcattg tcacctttgt ccagagagac ccaggcgatc
     6061 ggccaccgct ggcgtcgggc agcgtcctgc cactcgctca acagcgtcgt cttgccagag
     6121 ccggcggccg ccgagatgag ggtgagcctg tgttgcagcg cttcgtccag gtgtgcggtc
     6181 aggcgcgggc gccgcacgag ccgggcgcgc aggtgaggcg ggcgcagttt ggttgccagc
     6241 agcgagtgct gccgtgcgat gtcgtccagg gagtggagtg gctcactcat acgcgaacgg
     6301 ccttgccatc gatacaagca ggggcgcttg ctggcgtagt gtaactggaa cagcgcaaaa
     6361 cgcaaccgcg tctataccag tgcagcgcgt cgcgagtgaa gccgcg
//
LOCUS       DBNHXB010000285         3430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2256312, whole genome shotgun sequence.
ACCESSION   DBNHXB010000285 DBNHXB010000000
VERSION     DBNHXB010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2256312"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="AC162G_11535"
     CDS             <1..1668
                     /locus_tag="AC162G_11535"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5076496.1"
                     /translation="PISEPSQLPRDATEVLWNSLTRLPYKDNTQSPGVLLMFFVTWLF
                     GAVVSLTELRRRNIITPANQWRAAGLYAGVSIAFSFAYWIFHASGLLNVIGTLFRLNA
                     GPAASFDEYMGRFITTAEGFAGLLALFYVMVFAALIFLALSLYGEARARALPLAGEWG
                     VIAGVPIAAAALAMVFAFNYNSIRADIIYKQGAAWVANNACDGTGQPISQCDIAIAHL
                     KQALRHAPDEDFYMLALGASYLNKSAASTDEGSPLLIENSTYDSVFNLNAERTSQLNR
                     RDALTAARVTLERARRVNPLNTDHSANLARLHRRWSDLASSTDERRLRLDQSNKFYEQ
                     ATNLSPNNAQLWNEWALVHFALYEVASQLGDAATAQAALDEAGNKLDQSLALDQEFAD
                     TYFYLASLYTVRNEPEKAQAALEQALRLNPDNAEVWARLTDQLLASGNYTEADRLTQD
                     FLSRNPTFLPGWRTLAQHIYLPQDRLAEAANATQHALELSGQDPEHWLDQAAMAQILL
                     RAGDPAAALPYAQTAVQLAPPERQADAQAILDAVQAALSGSSQSPLP"
     gene            1691..2740
                     /locus_tag="AC162G_11540"
     CDS             1691..2740
                     /locus_tag="AC162G_11540"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIR5076497.1"
                     /translation="MTFATTAMLIFAGALILAISGTPLARRIAPRLGLIDQPNARKVH
                     QHPMPRMGGAAIVVASLAAILLFQGRFEFQQLVGILLGGSFVSLLGALDDRWGMHPIV
                     KLGGQFFAAAIPIAAGVVVQLFPEPWMNVAASALWIVGVTNAFNLLDNMDGLSSGICV
                     IAAAFFTVLASLSHQSYVAALSAAVLGASLGFLIYNFNPATIFMGDSGSLFLGFVLAT
                     LAIKLRFPDNVTFVTWMVPVLVLGVPLFDMTLVVVSRLRRGRNPLTTPGKDHTSHRLV
                     EAGFTTREAVLLLYLAGGAFGLLAIFVTQASVLEGYVVGIGAALVCLYALIRFEIQFH
                     SATRLAGRSQNTDHG"
     gene            2826..>3430
                     /locus_tag="AC162G_11545"
     CDS             2826..>3430
                     /locus_tag="AC162G_11545"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015666.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR5076498.1"
                     /translation="MSEQDQHLAVIGLGYVGLPLAVALAELGRTVTGLDIDQKKVASL
                     NRGSSHIPDVPGEAVRRVVEAGRFAATTDAEALRAPDAIFICVPSPFDANRAPDLSYI
                     RSAAQSVQPRLRKGHLIVLESTTYPGTTEEILQPMLESTGLRAGVDFDLAFVPERIDP
                     GQVESKGWTIKNTPKVVGALTPQGARRTAALLETMGAPVHVV"
BASE COUNT          579 a         1126 c         1101 g          624 t
ORIGIN      
        1 cccatctccg agccttccca gttgccgcgg gacgcgacgg aggtgctgtg gaactcgctg
       61 acgcggctgc catacaagga caacacgcag tcgcccggcg tgctcctgat gttcttcgtc
      121 acctggctgt tcggcgcagt ggtgtcgctg accgagctga ggcggcgtaa cattatcaca
      181 ccggcaaacc agtggcgcgc ggccgggttg tacgcaggcg tctccatcgc gttttcattc
      241 gcttactgga tattccatgc ctctggcctg ctcaacgtga tcggcacgtt gttcagattg
      301 aacgccgggc cggcagccag cttcgacgag tacatggggc gcttcattac caccgccgaa
      361 ggctttgctg gtctgctggc cctgttttat gtgatggtct tcgcagcctt gatctttttg
      421 gccctgagcc tgtacgggga ggcgcgcgcg cgtgccctgc cgctcgccgg cgagtggggc
      481 gtgatcgccg gcgtgcctat cgccgccgcc gcgctggcga tggtgttcgc cttcaattac
      541 aactccatcc gggcggacat catttacaaa caaggagcgg cttgggtcgc caacaacgcc
      601 tgcgatggca ccggccaacc gatcagccag tgcgacatcg ccatcgccca cctcaagcag
      661 gcgctccggc acgcgccgga cgaagacttt tacatgctgg cgctcggcgc atcgtacctg
      721 aacaagtcgg ccgcctcgac cgacgagggg tcgccactgc tcatcgaaaa cagcacgtat
      781 gactccgtgt ttaacctgaa cgccgagcgc acctcccaac tgaaccggcg cgacgcgctg
      841 acggctgcgc gcgtcacgct cgagcgcgcg cggcgggtga acccgctcaa cacggatcac
      901 tcggccaacc tggcgcgatt acatcggcgc tggtcggacc tggccagcag cacggatgag
      961 cgccgcctgc gcctcgatca gtcgaataag ttctacgagc aggcgaccaa cctcagcccg
     1021 aataatgcgc agttgtggaa cgaatgggcg ctcgtgcact tcgcgctgta tgaggtggca
     1081 tcgcagttgg gcgacgcggc cacggcgcag gccgcgctgg acgaggcggg caacaagctc
     1141 gaccagtcac tggcgctcga ccaggaattc gccgacacct acttctatct ggcgagcctg
     1201 tacacggtgc ggaacgagcc ggaaaaggcg caggcggcgc tcgagcaggc gctgcgcctc
     1261 aacccggaca acgccgaagt ctgggcgcgc ctgacggacc aactgctcgc gagcggtaac
     1321 tacacggaag ccgacaggtt gacgcaggat ttcctcagcc gcaacccgac ttttcttccc
     1381 ggctggcgca cgctggcgca gcacatttac ctgccgcagg accgcctggc cgaggcggca
     1441 aacgcgacgc agcacgcgct ggagctgagc ggtcaggatc cggagcactg gctcgatcag
     1501 gccgcgatgg cgcagatctt acttcgggca ggcgatccgg cagcggcgct gccgtacgcc
     1561 cagaccgccg tgcaattggc gcctccggag cgccaggccg acgcacaggc gattctggac
     1621 gctgtccagg cagcgctgag cggctcttcg cagtcgccgc tcccgtgagt gacgctcgga
     1681 ggatgcaggc atgactttcg cgacgactgc catgctcatt ttcgcgggtg cgctgatcct
     1741 cgccatcagc ggcacgccgc tggcccggcg catcgctccg aggctcggcc tgatcgacca
     1801 gcccaacgcg cgcaaagtgc atcagcaccc tatgccgcgc atgggcggcg cagccatcgt
     1861 cgtcgccagc ctggcggcga tattgctttt ccagggtcgg tttgagtttc aacagttagt
     1921 gggcatcctg ctaggcggct ctttcgtctc gttgttaggc gcgctggacg accgctgggg
     1981 catgcacccc atcgtcaagc tgggtggcca gttcttcgcc gccgcgatcc ctatcgccgc
     2041 cggcgtcgtc gtgcagttgt ttcccgagcc gtggatgaac gtcgcagcga gcgcgctgtg
     2101 gatcgtcggt gtcacaaacg cgttcaatct gctcgacaac atggacggcc tgtcgagcgg
     2161 aatctgcgtg attgcggcag ccttcttcac ggtgctcgcg tcgttgagtc accaatccta
     2221 cgtcgccgcg ctgtcggcgg ccgtgcttgg cgcatcgctg ggattcctca tctacaactt
     2281 caatccggcc acaattttca tgggcgactc cggcagcctg tttctgggat tcgtgctcgc
     2341 cactctggcc atcaagctgc gcttcccgga caacgtcacc ttcgtcacgt ggatggtgcc
     2401 cgtcctggtg ctgggcgtgc cgctattcga catgacgctg gtcgtcgtct cacggctgcg
     2461 gcgcggccga aacccgctga cgacgcccgg caaagaccac acgtcgcacc ggctcgtcga
     2521 ggcaggcttc acgacgcgcg aggccgtgct gctgctgtac cttgccggcg gcgcgttcgg
     2581 gctgctggcg atcttcgtca cacaggccag cgtactcgaa ggttacgtcg tgggaatcgg
     2641 ggccgcgctg gtgtgcctgt acgcgctgat ccgtttcgag atccagttcc atagcgcgac
     2701 tcgcttggcg ggccgcagcc aaaataccga ccatggatga cgcgcgttac actcacgcga
     2761 agcgtgcggc ggcagttgca ctgccggcgc acgcttcgcg caactgcggc aggaaatgag
     2821 agcatatgtc tgaacaagac caacacctgg ccgtcatcgg cctgggctac gtcggtctgc
     2881 cgctcgcggt cgctctggcc gagctagggc gcacggtgac cggcctggac attgatcaga
     2941 agaaggtggc gagcctgaat cgaggctcgt cgcacattcc cgacgtgccg ggcgaagcgg
     3001 tgaggcgcgt cgtagaggcc gggcgatttg ccgcgacgac cgacgcggag gcgctgcgcg
     3061 cgcccgatgc cattttcatc tgcgtcccca gcccgtttga tgccaatcgc gcgcccgatc
     3121 tctcgtacat tcgcagcgcg gcgcagtcgg tgcagccgag gctgagaaag ggccacctca
     3181 tcgtgctcga atcgaccacg tatccgggca caactgagga gattttgcag cctatgctcg
     3241 aatcgaccgg cctgcgtgcc ggcgtcgatt tcgacttggc gttcgtgccg gagcgcatcg
     3301 atcccggaca ggtagagagc aagggctgga cgatcaagaa cacgccgaaa gtcgtcggcg
     3361 cgctcacgcc gcagggtgcg cggcgcacgg cggcgctgct ggagacgatg ggcgcgccgg
     3421 tgcacgtcgt
//
LOCUS       DBNHXB010000286         2707 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1249427, whole genome shotgun sequence.
ACCESSION   DBNHXB010000286 DBNHXB010000000
VERSION     DBNHXB010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1249427"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1929
                     /locus_tag="AC162G_11550"
     CDS             <1..1929
                     /locus_tag="AC162G_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HIR5076499.1"
                     /translation="LVTPDTFTVNKATGQVMQRDIADKQVMTVRVEGGTREQPVPESL
                     RRVPVLDEDAAAQLNRLGVQIEQLYGMPMDIEWTLTDGRFAIVQARPITALSEPEPSA
                     PTTWKMPKGCYAAMRNNIVELMTEPLTPLFDTLGRSCINASMNRLITQFMGRPGILPA
                     EAIISVNGFAYYNGSLGLRPIGFVLLHSIGIMKRMFRGAAERWQVARPRYVSTVERWQ
                     SVAWREQSGAAILGGVRELMEAAIDAYFALVSGIIPGAWISEALFTFVYRFTNRRDDP
                     SAPTYLLGYDSVPIQAEKALYDLAEWVRTRTELAAYLSRTPAAQLAAQLDSEQVPADV
                     SADNWREWQSQFQAHLRRYGHAIYNLDFANPVPADDPTPLLDTCKMFINGQGMNPHER
                     QQAAADRREQATQAMLTRLKGLRLKLFRKYVASAQHYAPLREDGLADVGLSYPLLRQM
                     LRELGRRFSVGGLIEQPDDVFWLTQDEVEQAVARLDRGESLNSLSALVLQRKAAWRAA
                     RRLAPPMMLPHIKLFERIRTARKRRDGKDSLKGVAASPGRVTATARVLYGPEDFDQMQ
                     AGDVLVASITTPAWTPLFARAAAVVTDIGGPLSHGSIVAREYGIPAVLGTGVATRRIQ
                     SGQMITVDGSAGTVALNV"
     gene            2002..>2707
                     /locus_tag="AC162G_11555"
     CDS             2002..>2707
                     /locus_tag="AC162G_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR5076500.1"
                     /translation="MAMNLKSSEYVLPLADRQARLEIVGGKGASLARLANAGLPVPDG
                     FHVTTEAYRHFVAENNLQSDILAALEGVDLSRPSTLEEASHRIRERVAKAHIPPALAG
                     AIVEAYGALPGQNPAVAVRSSATAEDLPEASFAGQQETYLNVSGAEAVLEATRKCWTS
                     LWTARAIAYRANLSVSPERVGMAVVVQRLVPAEASGILFTANPMNGQRDQAVINATWG
                     LGEAIVGGLVTPDTFTV"
BASE COUNT          485 a          844 c          916 g          462 t
ORIGIN      
        1 ctggtcacgc ccgacacgtt cacggtgaac aaggcgaccg gtcaggtgat gcagcgcgac
       61 atcgccgaca agcaggtgat gaccgtgcgc gtcgaggggg gcacgcgtga gcaacccgtg
      121 ccggaaagcc tgcgtcgtgt gccggtgctg gacgaagacg cggccgcaca actcaaccgg
      181 ctgggtgtgc agatcgagca gttgtatggg atgccgatgg acatcgagtg gacgttgacc
      241 gatggcaggt tcgccatcgt ccaggccagg ccgatcacgg ccttgtctga gcccgaaccg
      301 tctgcgccga cgacgtggaa gatgcccaaa ggctgttatg cggccatgcg caacaatatc
      361 gtcgaattga tgaccgagcc gctgacgccg ctgttcgaca cgctgggtcg atcgtgcatc
      421 aatgccagca tgaaccggtt gatcacgcag ttcatgggca ggccgggcat cttgccggca
      481 gaggcgatta tcagtgtcaa cgggttcgcc tactacaacg gctcgctcgg tctgcggccg
      541 atcggctttg tgctcctgca cagcatcggc atcatgaagc gcatgttccg tggcgcggcg
      601 gaacgttggc aagtggcacg cccgcgctat gtgagcaccg tggagcgctg gcagtccgtc
      661 gcgtggcgcg agcaatcggg cgcggcgatt ctcggtggcg tgcgcgaact aatggaagcg
      721 gcgatcgacg cttacttcgc actggtgtcc ggcatcatac ccggcgcatg gattagcgag
      781 gctctgttca ccttcgtcta tcggttcacc aatcggcgtg acgatccatc cgctccgacc
      841 tatctactgg gctacgacag cgtacccatt caagccgaga aagctctgta cgacctggcc
      901 gaatgggttc gcacgcgcac ggagctggcg gcctatctga gtcgcacacc ggccgcacaa
      961 ctcgccgcgc agttggacag cgaacaagtg ccggcggatg tgtctgccga caattggcgc
     1021 gaatggcaga gccaattcca ggcgcacctg cggcgctacg gtcacgccat ctacaatctc
     1081 gactttgcga accctgtgcc ggccgatgat cccacgccac tgttggacac gtgcaagatg
     1141 ttcatcaatg gtcagggcat gaatccgcat gaacgccagc aggccgccgc cgatcggcgt
     1201 gagcaggcga cgcaggcgat gctgactcgc ttgaaggggt tgcgcctgaa actcttccgc
     1261 aagtacgtcg cgtctgcgca gcactatgct ccactgcgtg aagacggctt ggccgatgtc
     1321 ggcctgagct atccgttact gcggcagatg ctgcgcgagt tgggccgtcg tttcagcgtg
     1381 ggcgggttga tcgaacagcc cgacgacgtc ttctggctga cgcaagacga ggtcgaacag
     1441 gctgtggcgc ggctcgatcg cggcgagagc ctgaacagtc tgagcgcgct ggttctgcag
     1501 cgcaaggcgg cgtggcgggc tgcgcggcgc ctggcaccgc cgatgatgct gccgcatatc
     1561 aagttgttcg agaggatcag gacggcgcgt aagcggcgag acggcaagga ttcgctcaaa
     1621 ggcgtggccg ccagcccggg ccgcgtgact gccacggcgc gcgtattgta cgggccggaa
     1681 gacttcgatc agatgcaggc gggcgacgtg ctcgtggcgt cgatcaccac acccgcctgg
     1741 acgccgttgt ttgcccgcgc ggcagccgtc gtcaccgaca tcggcgggcc gctcagccac
     1801 ggctcgatcg tggcgcggga gtatggcatt cccgccgtgt tgggcacggg cgtcgcgacc
     1861 agacggattc agagcgggca gatgatcacg gtcgatggaa gcgcggggac ggtagcgctg
     1921 aatgtgtgaa gcgtgagggg tggtggagat cctgcatggg ccgcaccgac cgggtcatcc
     1981 cgcgccgggg gaggtagtat gatggcgatg aacttgaaat cctccgagta tgtgctgcca
     2041 cttgccgaca ggcaagctag gctggagatc gtgggcggca agggcgcgtc gctagcgcgg
     2101 ctcgccaacg ccggcctgcc cgtgcccgac ggctttcacg tgacgaccga ggcgtatcgg
     2161 cacttcgtgg ccgagaacaa cctgcagtcc gacatcctgg ccgcgctgga aggcgtggac
     2221 ctctcacgcc cctcgacgct tgaagaagcc tcccaccgca tccgtgaacg ggtcgccaag
     2281 gcacacattc cgccggcgct cgccggcgcc atcgtggagg cgtatggcgc tctacccgga
     2341 cagaacccgg ccgtcgccgt gcgttcgtcc gccaccgccg aggacctgcc cgaggcctcc
     2401 ttcgccggcc agcaggagac ctacctcaac gtcagtggcg ccgaggccgt gctcgaggcg
     2461 acccgcaaat gctggaccag cctgtggacg gcccgcgcca tcgcctatcg cgccaacctg
     2521 agtgtttcac cggagcgggt gggcatggcc gtcgtggtgc aacggctcgt cccggcggaa
     2581 gcctcgggca ttctgttcac ggcgaacccg atgaacggtc agcgcgatca ggccgtcatc
     2641 aatgcgacgt ggggactggg cgaagccatc gtcggcggcc tggtcacgcc cgacacgttc
     2701 acggtga
//
LOCUS       DBNHXB010000287         4550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1418159, whole genome shotgun sequence.
ACCESSION   DBNHXB010000287 DBNHXB010000000
VERSION     DBNHXB010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1418159"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="AC162G_11560"
     CDS             <1..501
                     /locus_tag="AC162G_11560"
                     /inference="COORDINATES: protein motif:HMM:NF044937.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl thiohydantoin desulfurase
                     domain-containing protein"
                     /protein_id="HIR5076501.1"
                     /translation="VPGTFSRALALGRAVQAARAAGYDPIAAAADTLGARHLIFGRIE
                     SLQEEERMGFYFTTATLPGLGSDTGQAARLVIKNEAMLCFLDERPVTIFPDPIYLLDP
                     QTGRGLMSVELAAGHEIVLLAAPAHPRLRAAAMTEAGRKAMSPARFGHPDLIYRPMEE
                     LLKGWT"
     gene            501..1190
                     /locus_tag="AC162G_11565"
     CDS             501..1190
                     /locus_tag="AC162G_11565"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIR5076502.1"
                     /translation="MDRQEVLDRIIAHRLIAIVRVADADHLLPVAEALQVGGAEVIEF
                     TLTTSGALEKLRAARRRFGETLTMGAGTVLDAGAAQSAIEAGAQFIVAPNLNPAVIRL
                     CHGPKYNVPVIPGALTPTEIIAAWDAGADLIKVFPASLGGPAYVREVLAPLPHVRLVP
                     TGGVTADDVVEFLSAGALAVGVGSALVDKMVVATSDWSALTERARRFVALISQAERVA
                     HSGQSAGDAQG"
     gene            1194..2162
                     /locus_tag="AC162G_11570"
     CDS             1194..2162
                     /locus_tag="AC162G_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR5076503.1"
                     /translation="MQKRYDVLTVGETMIRLSPPRWQRLEQADSFEVAVGGAESNVAV
                     CLSRFGLRVAWVSRLVDNPLGHRIMQTLRAQGVDVSHVIWTPMGRVGTYFVEFGQPPR
                     STNVIYDRRDSVMSEMTLSDLDPTLLNETRLVHLTGITPALSPACRDLVQGILKMAAE
                     RGVLRSFDVNYRARLWSADEAREALEPLCAGVDVLFVTRQDAHRLFGCAAGADETLRR
                     LQAKFNVGVAVLTMGEEGAMALNRDGSFYRARTEPAPEIDPLGSGDAFAAGFLYGYLT
                     EGDLEAALQIGVSAAALKRTIPGDLALITLEESLRGNARTSGQILR"
     gene            2309..3601
                     /locus_tag="AC162G_11575"
     CDS             2309..3601
                     /locus_tag="AC162G_11575"
                     /EC_number="5.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase family
                     protein"
                     /protein_id="HIR5076504.1"
                     /translation="MTIKTALQYGRYGLTVEIPASKVTLLEPQYIAGLADEAAEFRAA
                     VRHPIGARPISELIGTQDRVAVVIPDITRPFPSDRVLPWLLEELPHVPANRLTIHLGN
                     GSHRLETPDEIVALVGRGIAAGYRIVNHNACDRDSLELAGHMPDGRPVYMDTDYVRAD
                     RRIVLGFIEPHFMAGFSGGYKGIFPGVADIDAILHYHRASIIGDPRSTWGMLEDNPTQ
                     ELIRHNGSLLPVDFCINVTLNRQRQITRFFCGDPIAAHEAGCRFARATAMVACPHPYP
                     IVITTNSGYPLDQNLYQTVKGMSAGAQIVADGGLIITASECCDGFPAHGNYRKLLFEH
                     PSPKAILDTILAPGFLLYDQWEAQKHAEILLKARVALYSTIPADDARRAHLEPIDDLD
                     DFIAGEVRRLGPDAEVAVLPEGPMTIPYLANGGRATRL"
     gene            3897..4175
                     /locus_tag="AC162G_11580"
     CDS             3897..4175
                     /locus_tag="AC162G_11580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD family protein"
                     /protein_id="HIR5076505.1"
                     /translation="MAVTMRIPALWRPVVGANHVELEADNLSAALHALVGQFPLLGDQ
                     LFDERGEVREAINLFVNGEHVRYRGGLSAPLRDGDEVYIVPMISGGSR"
     gene            4234..>4550
                     /locus_tag="AC162G_11585"
     CDS             4234..>4550
                     /locus_tag="AC162G_11585"
                     /inference="COORDINATES: protein motif:HMM:NF014750.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5076506.1"
                     /translation="MAHGYMGEILNVDLTTGQIKAETLDETLCRDYIGGYGLGAKLLY
                     DRMPAHADPLGPDNILGILTGPLTGTPALIGSRFVVFAKSPKTQTWGDANCGGYFGPH
                     LKFA"
BASE COUNT          795 a         1482 c         1461 g          812 t
ORIGIN      
        1 gtaccgggca cgttcagccg cgcgctggcg ttgggccgcg cggttcaagc ggcacgcgcg
       61 gctggatatg atccgatcgc tgcagccgcc gataccctcg gcgcgcgcca cctgatcttc
      121 gggcgcatcg agtcgctgca ggaggaagag cgcatgggct tctacttcac gacggcgacg
      181 ctgcccggtc tcggctccga taccggacag gcagcgcggc tggtgatcaa gaatgaagcc
      241 atgctgtgtt ttctggacga acgcccggtg accattttcc ccgacccgat ctatctgctc
      301 gatccccaaa ccgggcgcgg cctgatgagc gtcgaactgg cagccggaca cgagatcgtc
      361 ttgctcgcgg cgccggccca tccccgcctg cgggcggcgg cgatgaccga ggccggtcgc
      421 aaagctatgt cgccggcgcg cttcggtcat cccgacctga tctaccggcc catggaagaa
      481 ctgttaaaag ggtggacgtg atggatcgcc aggaggttct ggatcggata atcgctcatc
      541 gcttgatcgc catcgtccgc gttgctgacg ccgatcatct gctgccggtt gccgaagcgc
      601 ttcaggtggg cggtgccgag gtcatcgaat tcacgctgac gacatccggc gcgttggaga
      661 aattgcgggc ggcgcgacga cgattcggcg agacgctgac catgggggcg ggcaccgtgc
      721 tcgatgcggg cgcagcacag agcgcgatcg aggccggggc gcagttcatc gtcgcgccca
      781 atctgaaccc ggccgtcatc cgcctgtgcc atgggccgaa gtacaacgtg cctgtcattc
      841 cgggggcttt gaccccgacc gaaatcatcg ccgcatggga tgctggtgcg gacctgatca
      901 aagtcttccc ggccagcctc ggcgggccgg cctacgtccg cgaggtgttg gcgccgctgc
      961 cccacgttcg gcttgtgccc accggtggtg tgacggccga tgatgtcgtg gagttcctga
     1021 gtgctggggc tctcgctgtg ggagtcggga gcgctttggt ggacaaaatg gtggtcgcca
     1081 ccagcgactg gtcggcgctg actgaacgtg cccggcggtt cgtggccctt atctcgcagg
     1141 ccgagcgcgt tgcacactcc ggacagtccg ccggcgacgc ccaaggctga tctatgcaga
     1201 aacgatacga tgttcttacc gtcggcgaaa cgatgatccg gctgtcgccg ccgcgctggc
     1261 agcgcctgga gcaagccgac tccttcgagg tggccgtggg cggtgcagag tctaacgtcg
     1321 ccgtgtgcct gagtcgcttt ggactacgcg tggcttgggt ctcgcggctg gtagacaatc
     1381 cactgggtca tcggatcatg caaacgttgc gtgcccaggg tgtagacgtg tcccatgtca
     1441 tctggacgcc gatggggcgc gtcggcacgt acttcgtgga gttcgggcag ccgcctcgct
     1501 cgactaacgt catctacgat cgccgggact cggtcatgag tgagatgacg ttaagcgatc
     1561 tggacccaac cctgctgaac gaaacgcgtc tggtgcacct gaccggcatc acgccggcgc
     1621 tcagcccagc gtgtcgcgat ctcgtccagg gaattctcaa gatggccgcc gagcgcggtg
     1681 tgctgcgctc ttttgatgtc aactatcggg ccaggctctg gtcggccgat gaggcgcgcg
     1741 aggcactgga gccactctgt gccggcgtgg acgtgttgtt cgtcacccgg caagacgcgc
     1801 atcggctgtt cggctgcgcc gctggtgcag atgagacact gcgtcggctc caggccaagt
     1861 tcaacgtggg agtggctgtg ctcacgatgg gcgaagaagg agcgatggcc ctgaatcgag
     1921 acggctcttt ctaccgcgcc cggacggagc cggcgcccga gatcgatccc ctgggcagcg
     1981 gcgacgcctt tgcggcgggt ttcctatatg gctacctgac ggagggcgat ctggaagccg
     2041 ctctccagat cggcgtgtcg gccgcagcgc tcaaacggac gatccccggc gatctggctt
     2101 tgatcacact cgaagagagc ctgcgcggca acgcccgtac ttcaggacaa atcctgcgct
     2161 gagtggggct ttcaaagtca ggggtctgcc cacgggaacc tctttgccgc tcacttgcat
     2221 gagtatataa tcacatcagc cgtctgcgcg catgcgccat tgggccgcgg cgacggggag
     2281 cgggggacga gtcatgcagg agatgatcgt gaccataaag accgcgctcc agtacggcag
     2341 gtatggcctg actgtcgaaa tccctgcctc gaaagtcacg ctccttgagc cgcagtacat
     2401 cgccggtctc gccgatgagg ccgccgagtt ccgcgcggcg gtccgccacc cgatcggcgc
     2461 gcggccaatt agcgaactga tcggcacgca ggatcgcgtc gccgtcgtca tccccgacat
     2521 cacccgcccc ttcccatcgg atcgcgtcct accgtggctg ctcgaagagc tgccgcacgt
     2581 tccggcgaac cgcctcacga ttcacctcgg caacggttcg caccgcctcg agacgccgga
     2641 cgagatcgtg gctctggttg ggcgcggcat cgccgcgggc tatcgcatcg tcaaccacaa
     2701 cgcctgcgac cgcgactcgc tcgagctggc cggtcacatg cccgacgggc gcccggtgta
     2761 catggacaca gactacgtgc gcgccgaccg gcgcatcgtc ctcggtttca tcgagccgca
     2821 tttcatggcc gggttctccg gcggctacaa gggcatcttc cccggcgtcg ccgacatcga
     2881 cgccatcctg cactaccacc gcgcgtcgat catcggcgat ccgcgcagca cctggggcat
     2941 gctggaagac aacccgacgc aggagctgat ccggcacaac ggctcactgc tgccggtcga
     3001 tttctgcatt aacgtgacct tgaaccgcca gcggcagatc acacgcttct tctgcggcga
     3061 tccgatcgcc gcgcacgagg caggctgccg cttcgcccgg gcgacggcga tggttgcctg
     3121 cccgcacccc taccctatcg tcatcacgac caacagcggc tatccgctcg atcagaacct
     3181 gtaccagacg gtcaagggca tgtcggccgg cgcgcagatc gtcgcggacg gtgggttgat
     3241 catcaccgcg tcggaatgct gcgacggctt ccccgcccac ggcaattacc gcaagctgct
     3301 gttcgagcac ccgtcgccca aggcgatcct cgacacgatc ctcgcgccgg gctttctgct
     3361 gtacgaccag tgggaggcgc aaaagcacgc cgagatactc ctgaaggcgc gggtggcgct
     3421 gtacagcacg atcccggcgg acgatgcccg ccgcgcccac ctggagccga tcgacgatct
     3481 ggatgatttc atcgccggcg aggtgaggcg gctcggcccg gatgccgagg tcgcggtgct
     3541 gcccgaaggg ccgatgacga tcccctatct ggcgaatgga ggacgtgcaa cgcgcctttg
     3601 aggtgcgccg cacgcagttg ataaacatgt catcgacgga agatgacaaa caacacttca
     3661 ttcccgcgcg ccacgtccga taatgaatga agatcgccgt tccattcctg cctagttgaa
     3721 tacatcctct cactcgagag gcgagcgcgt gaccgaactg cggcttgcag atgccaccag
     3781 cgcaaggggg gcaggccgat ccggttccgt ccttactcga actgaacgtt cattgtggtg
     3841 accggagcgt tgtgccaatg cggcataacg ctccggtcct gttttggagg cgtgacttgg
     3901 ccgtcacgat gagaatcccg gcgctgtggc gacccgtcgt cggggccaat catgtggaac
     3961 tggaggcgga taacctgtcg gcggcgttgc acgcgctggt cgggcaattc ccgctgctcg
     4021 gcgaccagtt attcgacgag cgcggcgaag tgcgcgaagc catcaacctg ttcgtcaacg
     4081 gcgagcacgt ccgctaccgg ggcgggctgt cagccccgct ccgcgatgga gatgaggtgt
     4141 atatcgtgcc gatgatcagc ggcggcagcc ggtgatcatc ccgacgctct tctgccaccc
     4201 gacggcagca gaccacacga aaggagatga cgtatggcac acggatacat gggcgagatc
     4261 ctcaacgtgg atctcaccac cggccagatc aaggcggaaa ccctcgacga gacactgtgc
     4321 cgggactaca tcggcggcta cggtctgggc gcgaaactgc tctacgaccg catgccggca
     4381 cacgccgacc cgctggggcc ggacaacatc ctgggtatcc tcaccggccc gctgaccggc
     4441 accccggcgc tgatcggctc acgcttcgtc gtcttcgcca agtcgcccaa gacccagacc
     4501 tggggcgacg ccaactgcgg cggctacttc ggcccgcacc tcaagttcgc
//
LOCUS       DBNHXB010000288         4760 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2089169, whole genome shotgun sequence.
ACCESSION   DBNHXB010000288 DBNHXB010000000
VERSION     DBNHXB010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2089169"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="AC162G_11590"
     CDS             complement(<1..752)
                     /locus_tag="AC162G_11590"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR5076507.1"
                     /translation="MSGPASTARTASPANSPAARRGALLALARLSRWTAILAVLALLV
                     VGAYTVVKPSLGTQAGPNTYRPAFGFAGQPAGRPRLPRVGIVAGHWGYDSGAVCADGL
                     TEQQITLDIALRAKRLLEARRLQVDLLQEFDARLNGYAADALVSLHVDSCEYINDIAT
                     GFKVARSVASYTPEAEDRLVNCLVESYGAATGLGYHENSITPDMTSYHTFSEVAPDTP
                     AAIIELGFLYLDRYFLTGQPDRAASGIADGIA"
     gene            856..1386
                     /locus_tag="AC162G_11595"
     CDS             856..1386
                     /locus_tag="AC162G_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR5076508.1"
                     /translation="MRQLSKTEIKRLQKQTAKTRPGKVALVFLLQNLDDPVNVGALFR
                     IADACGVREMILVGDTPAPPHPGISLAARGCERRVAWRHVNRIEEAAAALKGDGYHLV
                     ALELTRDAQMYTEYPYPDKVCLVLGSEGGGVWPKTLRLCDGTVFIPMFGKGPSLNVHV
                     AAAVVAYHILVSQRTE"
     gene            1733..2677
                     /locus_tag="AC162G_11600"
     CDS             1733..2677
                     /locus_tag="AC162G_11600"
                     /inference="COORDINATES: protein motif:HMM:NF013341.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase
                     domain-containing protein"
                     /protein_id="HIR5076509.1"
                     /translation="MLDYEAYEALFETRAERRRRTRPNGESKHKAPELAVAAAERFDE
                     TEYGFQPTYKSASNDHFEHQWIVEALSGFYYDKLIDDVLRVVKGGKEASVYCCAANAS
                     TGVEFIAAKIYRPRMFRSLKNDALYREGRSILDDTGKTIKARDKRVQRAVKNMTRFGS
                     ELRIGSWIGHEFETLRLLHDAGADVPRPYAQANNAIMMEYLGDGELAAPTLNHVALER
                     DEAQAVFERVMRNVELMLSRHRVHADLSAYNILYWDGAIKLIDFPQAVDPRINPHAES
                     LLARDVERVCQYFSRFRIKVDARRMARDLWSRYMHGEL"
     gene            2759..4045
                     /locus_tag="AC162G_11605"
     CDS             2759..4045
                     /locus_tag="AC162G_11605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5076510.1"
                     /translation="MEKLKAYRVFLAMSAAQAFAMTAMFTTGTVYYVNGIGLDPLQLV
                     LVGTALEATAFLFEIPTGIVADVYSRRLSILLGHFLLGAGFLLIGAFPLFDVLLAAQV
                     ITGIGYTFLSGATEAWLADEIGEVHVGAALIRARQLERITSIAGILAGAGLASLRLNL
                     PFLAGGGLLLALGAFLVWHMPETGFRPASREDRSTWSKFAGAFREGARLVRASRLLLL
                     FFGIAFFWGAASEGFDRLGDAHLLASFAFPAIGALQPVAWFGVLALAGTLIELAVNEL
                     LRKRLEAITRVPSATAKVLFVINALMIASVLVFALAGGFWLALSAMLLYGVCRSMGGP
                     LIDAWLVQHIDSSVRATVLSMRGQTDAIGQIAGGPGVGWIGRAVSLRAAIAAAGLLLS
                     PALILYARTIRHTREMLTRDALEEPKLIETMEATTS"
     gene            4216..>4760
                     /locus_tag="AC162G_11610"
     CDS             4216..>4760
                     /locus_tag="AC162G_11610"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5076511.1"
                     /translation="MHNVIESLPGCEPEIGRWLWALEDTRRRTKRSLEGLTQAVIDWP
                     HPLAPSPEVYSSTSGEGESDSPLLTTAVSSSGEGSGVRPGNSIGTLLYHLAVIEIDWL
                     HADVLQKPWPPELETLFPFDVRDEQAHLIAVRGLSLDAHLRRLGAGRSILLDAFRGMS
                     LDEFRRARRTQGYDVTPEWVI"
BASE COUNT          836 a         1486 c         1581 g          857 t
ORIGIN      
        1 cacgcaatgc cgtcggcgat gccgctcgcc gcgcgatccg gctgaccggt gaggaaatac
       61 cggtcgaggt acagaaagcc gagttcgatg atggccgccg gcgtgtcggg cgcgacttcg
      121 ctgaacgtgt ggtagctggt catgtccggg gtgatgctgt tttcgtgata gccgaggccg
      181 gtcgccgctc cgtacgattc gaccaggcag ttcaccaggc gatcctccgc ttcgggcgtg
      241 tacgaggcga ccgagcgcgc caccttgaag ccggtggcga tgtcgttgat gtattcgcac
      301 gaatcgacgt ggagcgacac cagcgcatcg gcggcgtagc cgttcaggcg cgcgtcgaac
      361 tcctggagca gatcgacctg gagccggcgc gcctcgagca gtcttttcgc gcgaagcgct
      421 atgtcaagcg taatctgctg ctctgtcagc ccgtcggcgc aaaccgcgcc cgagtcgtac
      481 ccccagtgcc cggccacgat gccgacccgt ggaaggcgcg gacgcccggc gggctggccg
      541 gcgaatccaa acgccggccg gtaggtattc ggcccggctt gcgtgcccag agacggcttg
      601 acgaccgtgt acgctccaac gacgagcagc gccaggacgg ccagaattgc cgtccagcgc
      661 gagagacgcg ccagagccag gagggcgcca cggcgggcgg ctggcgaatt tgcagggctt
      721 gccgtgcgtg ccgtgctcgc agggccggac atggcgcaaa taatactgcg aaaacaggcg
      781 ttcgtcaaaa agcaaatgca aaaattgtga taagatgcac cgatagcgtc gcaatagcag
      841 gcgacttgga atccaatgag acaattgagc aaaaccgaga tcaagcggct gcagaagcag
      901 accgcgaaaa cgcgccctgg taaggtcgct ctcgtttttc tgttgcaaaa tctggacgat
      961 ccagtcaacg tcggcgctct gtttcgcatc gccgacgcgt gcggcgtgcg cgagatgatt
     1021 ctggtgggcg acacaccggc gccgccgcac ccgggcatca gtttggcggc gcgcggctgc
     1081 gagcgccgcg tcgcgtggcg ccacgtgaat cgcatcgaag aggcggctgc cgctctgaag
     1141 ggcgatggct atcatctcgt cgcgctcgag ctgacccgcg acgcgcagat gtacacggag
     1201 tatccgtatc cggacaaggt gtgcctggtg ctgggcagcg aaggcggcgg cgtgtggccg
     1261 aagacgctgc gcctgtgcga cgggacggta ttcattccga tgttcggcaa aggcccgtcg
     1321 ctcaacgtgc acgtcgcggc ggcggtcgtt gcgtaccaca ttcttgtgag tcagcgcaca
     1381 gagtaattgg ggattggtaa ttaccactcc ccaattaccg ctccccaatt accgtgcaat
     1441 gccagatttt ctaacttgac ttcatgatcg aagcatgcta aactgcggcc atcctgacga
     1501 aaggaggcgc acacgatgtc atcacaattt gcccttgtct cgatcgaatg cgtcggagtg
     1561 cgcctcgtca ccgcttaggt atccctcatt gcttcctcgc ggccacgggt gcgctcctgc
     1621 ccgtggccgt tttgttttca aagacccttc gggtcttgga gacccgaaaa gtctggcacg
     1681 gccatggtgc gaattcgcac cgtggccgcc ttgttaagga gcctagccaa ccatgttgga
     1741 ctacgaggct tacgaagcct tattcgagac gcgcgccgag cggcgtaggc gcacacgtcc
     1801 caacggcgaa tctaaacata aagcgcccga gctcgccgtc gcagcggccg aacgcttcga
     1861 cgagacggag tacgggttcc agccgactta caaatccgcg agcaacgatc acttcgagca
     1921 tcagtggatc gtcgaggcgc tgagcggctt ctactacgac aagctgatcg acgacgtgct
     1981 gcgcgtcgtg aagggcggca aggaggcgtc ggtctactgc tgcgcggcga acgcctcgac
     2041 cggcgtcgag ttcatcgccg ccaagatcta ccgcccacgc atgttccgca gcctgaagaa
     2101 cgacgccctg taccgcgaag ggcgcagcat tctcgacgac acgggcaaga cgatcaaggc
     2161 ccgcgacaag cgcgtgcagc gcgccgtcaa gaacatgacg cgcttcggca gcgagctgcg
     2221 catcggatcg tggatcggtc acgagttcga aacgctgcgc ctgctgcacg acgccggcgc
     2281 agacgtcccc aggccgtatg ctcaggccaa caacgccatc atgatggagt atctcggcga
     2341 cggcgagctg gccgcgccga cgctcaacca cgtggcgctg gagcgggacg aagcgcaggc
     2401 ggtcttcgag cgtgtgatgc gcaacgtcga gttgatgctg tcccggcacc gcgtccacgc
     2461 cgacctgtcg gcctataaca ttctgtactg ggatggcgcg atcaagctca tcgacttccc
     2521 gcaggcggtc gacccgcgca tcaatccgca cgccgaatca ctgctggcgc gcgacgtcga
     2581 gcgcgtgtgc caatatttct cgcgcttcag gatcaaggtg gacgcgcggc gcatggcccg
     2641 cgatctgtgg tcgcgctaca tgcacgggga gctatgatcc acgaaggaca cgaaggggca
     2701 cgaagatcaa aaccttagtg gttcttcgtg tgctttcgtg gatcgccaag agagaatcat
     2761 ggagaaactg aaagcctatc gggtgtttct ggcgatgagc gcggcgcagg cgtttgcgat
     2821 gacggcgatg ttcacgaccg gcacggtgta ctacgtcaac ggcatcggcc tcgatccgct
     2881 gcaattggtg ctggtcggca cggctctgga agccaccgca ttcctgttcg agataccgac
     2941 cggcatcgtc gccgacgtgt acagccgccg cctgtcgatc ctgctcggcc acttcctgct
     3001 gggcgcaggc tttctgctga tcggcgcgtt cccgctgttc gacgtgctgc tcgccgcgca
     3061 ggttatcacc ggtatcggct acaccttcct cagcggcgcg accgaagcct ggctcgccga
     3121 cgagatcggc gaggtgcatg tcggcgcggc gctcattcgc gccaggcaac ttgagcgcat
     3181 aacgagcatc gccggcatcc tggccggcgc ggggctggcg agcctgcgcc tgaacctgcc
     3241 gttcctcgcc ggcggcgggc tgctgctggc gctgggtgcg ttcttggtgt ggcacatgcc
     3301 ggagaccggc ttccggccgg cgtcgcgcga agatcgctcg acgtggagca agttcgccgg
     3361 cgcgttccgc gagggtgcgc ggctggtgcg cgccagccgg ctgctgctgc tgttcttcgg
     3421 catcgccttc ttctggggcg cggcgagcga aggcttcgac cgtctcggcg acgcgcacct
     3481 gctggcgagc ttcgccttcc cggcgatcgg cgcgctccag ccggtggcgt ggttcggcgt
     3541 cctcgccctc gccggcacgc tgatcgagct ggccgtgaac gagcttctcc gcaagcggct
     3601 ggaggcgatc acgcgcgtcc cgtctgcgac ggcgaaagtg ctgttcgtca tcaacgcgct
     3661 gatgattgcc agcgtgctcg tcttcgccct ggcaggtggg ttctggctgg ccctctcggc
     3721 gatgctgctg tacggcgtgt gccgatcgat gggtgggcca ctgattgacg cgtggctggt
     3781 gcagcacatc gattcgagcg tgcgggccac ggtgctgtcg atgcgcgggc agaccgacgc
     3841 catcggccag atcgcgggcg ggccgggcgt tggctggatc gggcgcgccg tgtcgctgcg
     3901 cgcggcgatc gcggcggcgg gcctcctgct gtcacccgcc ctgatcctgt acgcgcggac
     3961 gatccggcac acccgcgaga tgctgacccg cgatgcgctg gaagagccga agttgatcga
     4021 gacgatggag gcgacgactt cgtagtggcg actttacggc ggtttccact ttgatttacg
     4081 atagcatcct gagcggactg gcgagcgcat tctgagcgga acgcagtgca gtcgaagagc
     4141 ctgccccaag cgaaggcgaa gggataacga ccgaagtcgt cactacggac gtcactgtga
     4201 gttacggaga ggagaatgca caacgtcatc gagtcactgc cgggctgcga gccggagatc
     4261 ggtcgctggc tgtgggcgct ggaggacacg cgccgccgca ccaagcgcag cctcgaaggg
     4321 ctgacgcagg ccgtcatcga ctggcctcac cctctggccc cctctcccga agtatatagt
     4381 tccacttcgg gggagggaga atcggactcc cctctcctga cgacggctgt ttcgtcgtca
     4441 ggagaggggt cgggggtgag gcctggcaac tcgatcggca cgctgctgta ccaccttgcg
     4501 gtgatcgaaa tcgactggct gcacgccgac gttttgcaga agccgtggcc gccggagctg
     4561 gaaacgctgt tcccattcga cgtgcgcgac gagcaggctc acctgatcgc ggtgcgcggg
     4621 ctgagtctcg acgcgcacct gcgccgtctc ggcgcgggtc gctcgatcct gctcgatgcg
     4681 tttcgcggca tgtcgctcga cgagttccga cgcgcgcggc gcacgcaggg ctacgacgtg
     4741 acgcccgaat gggtcatcca
//
LOCUS       DBNHXB010000289         7048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1752902, whole genome shotgun sequence.
ACCESSION   DBNHXB010000289 DBNHXB010000000
VERSION     DBNHXB010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1752902"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..389
                     /locus_tag="AC162G_11615"
     CDS             90..389
                     /locus_tag="AC162G_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HIR5076512.1"
                     /translation="MLKKSYPKNKNTCRVTFELPGAAGAQTACLVGDFNHWDKTAHPM
                     KKRKKDGAFTATIELARDQIFHFRYWLDNSRWENDWAADAYVPGPFGDENSVVYT"
     gene            complement(437..1927)
                     /locus_tag="AC162G_11620"
     CDS             complement(437..1927)
                     /locus_tag="AC162G_11620"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5076513.1"
                     /translation="MSVTRQQIYIVVVGAIGIAVLIGSLVAPPAAPLVLLLFCILAAA
                     AEWTTATLLQAKQFSIELSITSAVSFAAALIFAPANAALVGAVGGATAAVISARRQKL
                     NRQRAVEVLVFNAGMNALATLVGALVFQSAHGGPLTGNLILTDLPLLLAAALADDLVN
                     AVALVVMIAIQWAQKPFTIWRENFAWAAPINVATMAVGGGALALGYEALGLTGLLVFF
                     LPVFLSVYAFRAFITRTQTMMAHLEEMVRERTADLAKANESLQELHRQKDAFFAIITH
                     DMRTPLTSMLGFADLLERMGRLDPGAQPFIGAIKRNTESLMEMVANILDLSRLDSGRM
                     EYKREPVDLCALVDEVLANIDGHSRARHISVHRALEATEKLTGDAGKLRRMITNLVSN
                     AIKYTPEGGQVYVDVRQVNGHVRFSVRDTGVGIPADQLPYIFERFRRVRHSGGSDEVG
                     TGLGLAIVQEFAQAHGGRIEVESEEGVGSTFTVTLPVKHEPSSAEN"
     gene            complement(2213..3862)
                     /locus_tag="AC162G_11625"
     CDS             complement(2213..3862)
                     /locus_tag="AC162G_11625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR5076514.1"
                     /translation="MPAHFAGRRSHVLVVAALCAAIALSALGFATAAPAQSVRAIVQG
                     RSIAEVESAVQALGGFVTHRFALIQAVSADVPESALGALRARGLGVTLDTPVRAAGRP
                     DADSSGFAEAVGADELWAQGYTGQGVGVAILDTGIARHPDLLRDAQGRPAQRVVARYD
                     ALDAGSGAPDLFGHGTFMAGMIGNARRTANGFAGVAPGTRLIDVRVMDASGQGTQTDL
                     IEGIEWVVAHKDEYNIRVMNLSLFAYVTGPYWASPVNQTVEAAHQAGIVVVAAAGNMG
                     DANMTIAMPGNDPFAITVGAFTDNYTPNNPGDDTIPPFSSTGPTETGFVKPELLAPGA
                     HVVSTMLDGAAWAREHRDNRVRGGYYEAAGTSTATAVTSGVAALLLEAHPDWTPSQLK
                     HHLMITARPIEGASLWRQGAGRVWAPDALADTQAGEANPGMIPGQPYVGPTVYLFDQF
                     TLVYEDGTLVAPQGYNWTGGYNWTGGYNWTGGYNWVGSLDWQEWVEGTLWQSGYNWTG
                     GYNWTGGYNWTGGYNWTGGYNWTGGTNGPGGYNWTGVQLLP"
     gene            4156..5349
                     /locus_tag="AC162G_11630"
     CDS             4156..5349
                     /locus_tag="AC162G_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076515.1"
                     /translation="MSRTGRFALLLAAAALLSLAPIQAPTFAQIATPAAPNLLINGGL
                     EGQYVMQCSARSGPPWVAVPCGDPIDFGTTMLWQTAQVPVGWTAWWLPPNENRSDPNF
                     YSTYPNQCYAEDAPRDCVAWHNPEYRDTAGGPQGPPLRKLAGDNSQKYFTFYSLHEAG
                     LYQTVGGLEPGQRVRFSVYMQAWSNAESDPFVSAGQPTMGMRVGIDPHGGNFPWSPSI
                     VWSPVKEAFDHWELFTVEAVAQSDRVTVFTRSRPVFAIQHNDVYVDEASLIVVGSAPA
                     QASPTSASEATRASAATPTRTPSPTWTPSPTRTVTPTRTPFHVPTRTRTPTPSATFTP
                     SATPLPTSTPTATATATPTLPAVFATAPSGSEPVLWAGGVIAGILLVAFVIVRIGSSG
                     VRDET"
     gene            complement(5544..6902)
                     /locus_tag="AC162G_11635"
     CDS             complement(5544..6902)
                     /locus_tag="AC162G_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR5076516.1"
                     /translation="MPPTLPVDSIHAARRALQSGEIAPGDLTASTLDRIRALNPKLNA
                     YLEVFDPPERAANPDGPLGGIPIAIKDLIDVAGRRTTAGSPFMIDNVAAEDATVVKKL
                     RAAGAVLAGKTHLHEWAHGTTNDNPHFGPCRNPWDLERVSGGSSGGSAVAVAAGLCLG
                     ALGTDTGGSIRIPASLCGIVGLKPTRGRVSLRGVVPLSWSLDHVGPMARTVRDAAILL
                     GAIAGHDPEDPASIDAPVPDYAGSLDGETGLRGARIGVPEALFFEESEPEVVEVVRAA
                     IEALRSLGGTIVEVELREAADMTKATGVILMADAAAFHRERFESQPDKFGADVLDRIK
                     AGRAFTATDYAFAVQAGQQWRHRLRQVFESVDVLATPATQIAAPRIGEPEAGARLPRF
                     TRLFNLAGTPAISVPCGFVSGGRLPIGMQLVAKWWDEATLLRVAHAYERATEWHLRKP
                     SL"
BASE COUNT         1224 a         2288 c         2321 g         1215 t
ORIGIN      
        1 gggggattgc ttcgcagaaa gatgctcgca atgacgttga gctgagtagt tacgagctgc
       61 caagacaaac tcggttcaca gggggcgcta tgctgaagaa atcctatccg aagaacaaga
      121 acacttgccg cgtgacgttc gagctgccag gcgcagccgg cgcgcagacc gcgtgcctgg
      181 tcggcgactt caaccattgg gacaagactg cgcaccccat gaagaagcgc aagaaggacg
      241 gcgccttcac cgcgacgatc gagctggcgc gcgaccagat cttccacttc cgctattggc
      301 tcgacaattc gcggtgggag aacgactggg cggcggacgc ctacgtgccc ggtcccttcg
      361 gcgacgaaaa ctcggtggtc tatacctgac tgttcgagac ctgccaggtt tccgcaaacc
      421 tggcaggtct tttatttcag ttctcggcag aggatggctc gtgcttcaca gggagagtca
      481 cggtgaacgt gctgccgacg ccctcttcgc tctcgacctc gattcgtcca ccgtgcgcct
      541 gggcgaactc ctgaacgatc gccagaccca ggccggtgcc gacctcgtcg cttccgccgc
      601 tgtggcgcac acggcgaaac cgctcgaaga tgtacggcag ttgatcggct gggatgccga
      661 cgccggtatc gcgcacgctg aaccggacgt ggccgttcac ctggcgcaca tccacgtaga
      721 cctggccgcc ttcgggtgtg tacttgatcg cgttggacac cagattggtg atcatgcggc
      781 gcagcttccc tgcgtcgccg gtcagcttct cggtggcctc cagcgcgcga tggacggaga
      841 tgtggcgggc gcgggagtgc ccgtcgatgt tagcgagcac ttcgtcgacc agcgcgcaga
      901 ggtccacagg ctcgcgcttg tattccatcc ggcccgaatc gagcctggac aggtcgagga
      961 tgttggcgac catctccatg agcgattcgg tgttgcgctt gatcgcgccg ataaacggct
     1021 gcgcgcctgg atcgaggcga cccatgcgct ccagcagatc ggcgaagccc agcatgctgg
     1081 tcaggggtgt gcgcatgtcg tgtgtgatga tggcgaagaa ggcatctttt tgccggtgca
     1141 gctcctgcag cgactcgttg gccttggcga gatcggcggt gcgctcccgc accatctcct
     1201 cgaggtgggc catcatcgtc tgcgtgcggg tgatgaaggc gcgaaaggcg tacaccgaca
     1261 ggaacaccgg caggaagaac accagcaggc cggtcaatcc gagcgcctcg tagccaaggg
     1321 ccagcgcacc gccgccgacg gccatcgtcg ccacgttgat cggggcggcc caggcgaagt
     1381 tctcgcgcca gatggtgaac ggcttttgcg cccactggat cgcgatcatg acgacgagag
     1441 cgacggcgtt caccaggtcg tctgccaggg cagccgccag cagcaaaggc aggtcggtca
     1501 agatcagatt gccggtgagg gggccgccgt gcgccgactg gaagacgagc gcgccgacca
     1561 gcgtggccag cgcgttcatg ccggcattga agaccaggac ttcgaccgcg cgctgccggt
     1621 tgagcttctg ccggcgcgcg ctgatgacgg cggccgtggc gccgccgacg gcgccgacca
     1681 gggcggcgtt cgccggcgcg aagatcaacg ccgcggcgaa gctgacggcc gacgtgatcg
     1741 acagctcgat gctgaactgc ttggcctgca gcaatgtcgc cgtcgtccat tcggcggcgg
     1801 cggcaaggat gcagaacaaa agcagcacga gcggcgccgc gggcggcgca accagcgacc
     1861 cgatcagcac ggctatgccg atggccccga ccaccacaat atagatctgc tgccgggtga
     1921 cgctcatgct gttacaccat gtctcgccct gcctctattg tagtgaaagt ctccaggtgg
     1981 ttatatgcag atatattaca agaccgttgg cgcgaaaacg ctctcaactc cttccaatcc
     2041 gctccgatcc gccgcttcga gcttacgagc gcagccggat atttgtgaac cggagtttga
     2101 agactgcact ccgggaatgc gtcagactcc ggttgtgaag atagagccta aatgacaaca
     2161 gggatgcgtc agcgcacccc tgtgtgagtg gagagtcttg tggcttcgcc aatcacggaa
     2221 gtagttggac tccggtccag ttgtatccgc ccggcccgtt ggttccgcct gtccagttgt
     2281 acccgcctgt ccagttatac ccgcccgtcc agttataccc gccggtccaa ttgtagccgc
     2341 cggtccagtt atagcccgac tgccacagcg ttccctccac ccattcctgc cagtcgaggc
     2401 tgcctaccca gttgtagccg ccggtccaat tgtacccgcc ggtccagtta tatccgcccg
     2461 tccagttgta accttggggc gcgacgagcg tcccgtcttc gtacacgaga gtgaactgat
     2521 cgaacaggta gacggtcggc cccacgtaag gctggccagg gatcatgccc gggttagcct
     2581 caccggcctg tgtgtcggcc agcgcgtcag gcgcccagac ccggcccgcg ccctgccgcc
     2641 acagcgaggc gccctcgatc ggccgggccg tgatcatcaa gtgatgcttg agttgcgacg
     2701 gcgtccagtc aggatgcgct tcgagcaaca gcgcggccac accggaggtc accgccgtcg
     2761 cggtggatgt gcccgccgct tcgtagtagc ccccccgcac ccggttgtcg cgatgctccc
     2821 tcgcccacgc cgcgccgtcg agcatcgtcg agacgacgtg cgcgcccggg gccagcagct
     2881 ccggcttgac gaagccggtc tccgtcggcc cggtggacga gaagggcggg atggtgtcgt
     2941 cgcccggatt gttgggcgtg tagttgtcgg tgaacgcgcc gacggtgatg gcgaaaggat
     3001 cgttgccggg catggcaatg gtcatgttcg cgtcgcccat gttgccggcg gcggcgacga
     3061 cgacgatgcc ggcctggtgc gccgcttcga cggtctgatt gaccgggctg gcccagtacg
     3121 ggccggtgac atacgcgaag agtgagaggt tcatcacgcg gatgttgtac tcgtccttgt
     3181 gcgcgacgac ccactcgata ccttcgatca gatcggtctg cgtgccctga ccgctcgcgt
     3241 ccatcacgcg cacgtcgatc aggcgcgtgc caggtgcgac gccggcaaaa ccgtttgccg
     3301 tgcgccgggc attgccaatc atgccggcca tgaaagtccc atgaccgaac aggtcgggcg
     3361 cgcccgaccc ggcgtcgagg gcatcgtacc gggcgacgac acgctgcgcc ggacggccct
     3421 gcgcgtcgcg caagagatcg ggatggcgcg caatgcctgt gtcgagaatg gcgacgccga
     3481 caccttgacc tgtgtagcct tgcgcccaca gctcgtccgc gccgacggct tctgcaaatc
     3541 ccgaggagtc ggcgtcaggc cggcccgctg cgcgcacggg cgtgtccagc gtcacgccca
     3601 gaccgcgagc gcgcagcgcg ccgagggccg attccggcac gtcggccgac acagcctgaa
     3661 tcagcgcgaa ccggtgcgtg acgaagccgc cgagcgcctg aaccgcgctc tccacttcgg
     3721 cgatcgatcg tccctggaca atcgcgcgca ccgattgcgc cggtgcggcg gtggcgaagc
     3781 cgagcgcgct gagcgcgatc gccgcacaga gtgctgctac aacgagaacg tgagatcgcc
     3841 ggccggcgaa gtgtgctggc atgccagtcc tcccgctgtt tcttcgatat gactatagcc
     3901 gaaaccggcg ccggggtgcg ttgggggtcg gagagagacc cgttggagat cgcacgccag
     3961 caaacctcac cagacgcttc cagatcgtgc ggttcgatgc gacgggttgg ggcaggattg
     4021 aaagcggttt gagcgctctg agtggtggtc tcgaatcgcg cgcgccatcg cgcagcacga
     4081 acatcgattc tggtataata tcgccgagtt cgctccgggc gaaacacact tttttctgtt
     4141 gaaaagattc caactgtgtc gaggacgggt cgcttcgctc tacttcttgc cgctgctgcg
     4201 ctgctgagcc tggcgccgat tcaggcgccg acgttcgcgc agatcgccac accggccgct
     4261 ccgaacttgc tgatcaatgg cgggctggag ggccagtacg tgatgcagtg cagcgcgagg
     4321 agcggcccgc cgtgggtggc cgtgccgtgc ggcgatccga tcgacttcgg gacgacgatg
     4381 ctgtggcaga cggcacaggt gcccgtggga tggacggcgt ggtggctgcc gccgaatgag
     4441 aatcgcagcg atcccaattt ctacagtacc tatccgaatc aatgctacgc cgaggacgcg
     4501 ccgagggact gcgtggcgtg gcacaacccg gagtatcgcg atacggccgg cgggcctcaa
     4561 ggcccaccgc tgcgtaaact ggccggagac aactcgcaaa agtacttcac gttctacagc
     4621 ctgcacgaag cggggctgta ccagaccgtc ggcggcctgg agccgggcca gcgcgtgcgc
     4681 ttcagcgtgt atatgcaagc gtggtcgaac gcggagagcg atccgttcgt ttcggccggc
     4741 cagccgacga tgggcatgcg cgtcggcatc gatccccacg gcggcaactt cccgtggagt
     4801 ccgagcatcg tctggtcgcc ggtcaaagag gcgttcgatc actgggagtt gttcacggtc
     4861 gaggccgtcg cgcagtcgga tcgcgtgacg gtgttcacgc gctcgcggcc ggtgttcgcg
     4921 attcagcaca acgacgtgta cgtggacgag gccagcctga tcgtggtcgg cagtgcgccc
     4981 gcgcaagctt cgcccacttc ggcgagcgaa gcgacgcgcg cgtcagcggc gactccgacg
     5041 cggacgccca gcccgacatg gacgcccagc ccgacccgga ccgtcactcc cactcgaacg
     5101 ccgttccacg tgccgactcg cacacgcacg ccgacgccgt cggcgacgtt cactccgagc
     5161 gcgacgccgc tcccgacgag cacgccgact gcgaccgcca cggcgacgcc gactctgcct
     5221 gccgtgttcg ccacggctcc cagcggatcg gaacctgtgt tgtgggcggg cggcgtcatc
     5281 gccggaatac tgctcgtcgc cttcgtcatc gtgcgcatcg gttcttcggg ggtgcgcgac
     5341 gagacgtagc ctgtcgttct gcgcttgaag gtggcttcac ttcgcggaag tcggatctgc
     5401 gcagaggctg tctgaaaact caccaccaag cctgccctga gcggagccga agggacacca
     5461 agacacgagg aaaatccttg agattcttgg tgtcttggtg tcttcgtggt tattgtagcg
     5521 actattgcag acaggacctg agtctaaagc gagggcttcc gtaggtgcca ctcggtcgcg
     5581 cgctcgtagg catgcgcgac gcgcagcagc gtcgcctcgt cccaccactt ggcgacgagc
     5641 tgcatgccga tcggcagccg cccgcccgac acgaacccgc acggcacgga gatcgccggc
     5701 gtgcccgcca ggttgaacag ccgcgtgaag cgcggcaggc gcgcgcctgc ttccggctcg
     5761 ccgatgcgcg gagccgcgat ctgcgtcgcc ggtgtggcga gcacgtcgac agactcgaag
     5821 acctggcgca gccggtgtcg ccactgttgc ccagcctgca cggcgaatgc gtagtccgtc
     5881 gcggtgaaag cacggcctgc cttgatgcgg tccagcacgt cggcgccgaa cttgtcgggc
     5941 tgcgactcga accgctcacg atgaaacgcg gcggcgtcgg ccatcaggat gacgcccgtc
     6001 gctttcgtca tgtccgccgc ctcgcgcaac tccacttcga cgatcgtccc gcctagactt
     6061 cgcagcgcct cgatcgccgc gcgaacaact tcgaccacct ccggctcgga ctcctcgaaa
     6121 aagagagctt ccggcacgcc gatgcgcgct ccgcgcagcc ctgtctcgcc gtcgaggctc
     6181 ccggcgtaat cgggtaccgg cgcgtcgatc gacgccgggt cttcgggatc gtgcccggcg
     6241 atcgctccca gcaggatggc tgcgtcgcgc acggtgcgcg ccatcggccc gacgtgatcg
     6301 agcgaccacg acagcggcac gacgccgcgc aggctgaccc gcccgcgcgt cggcttgagg
     6361 ccgacgatgc cgcacagcga ggccgggatg cggatcgacc cgccggtgtc ggtgcccagc
     6421 gcgcccaggc acagaccggc ggcgacggcg accgccgacc cgccgctcga cccaccggac
     6481 acgcgctcga ggtcccacgg attgcgacag gggccaaagt gcgggttgtc gttcgtcgtg
     6541 ccgtgcgccc actcgtgcag atgagttttg ccggcgagaa cggcgcctgc ggcgcgcagt
     6601 ttcttcacga cggtcgcgtc ttcggcggcc acgttatcga tcatgaacgg cgatccggcc
     6661 gtcgtgcgac gaccggcgac gtcgatcagg tctttgatcg cgatgggaat gccgcccagc
     6721 ggcccgtccg ggttggcggc acgctccggc ggatcgaaga cttcgaggta agcgttcaat
     6781 ttcgggttga gtgcccgaat gcgatcgagg gtggaggcgg tcagatcgcc gggcgctatc
     6841 tcgccggact gcaaggcacg acgagcggcg tggatcgaat cgacaggcag ggtgggtggc
     6901 attctagact ttatcggaaa tagtcgcatg gccccttgtg ggcgtcgaaa tcgtgcgaga
     6961 acgcgcacaa ggcgctatgc tacaccgtta tttccgaagt atagccctca cccggcgggc
     7021 tatttcagag gaatagccgg aaggcgtt
//
LOCUS       DBNHXB010000290         2520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1082094, whole genome shotgun sequence.
ACCESSION   DBNHXB010000290 DBNHXB010000000
VERSION     DBNHXB010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1082094"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="AC162G_11640"
     CDS             <1..809
                     /locus_tag="AC162G_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076517.1"
                     /translation="QAARALDAGPSTLPVAASGLSIQDLDPQTFDLLLRRRDFKVKWF
                     DCRSAILFPAGGAPARYLEPDFFSCDPDLMSHYLPGAPIIAQPRWPDSGNVIFTLRQF
                     DARALLDGALPRIAAQPIYIGSESFDAADPAGGLEPLALPIDLSGLQLLGWEMERDTW
                     KPGETIQIRTYWKVTAALAPPLSLFVHVTRPDGSIFAQHDGLDIGVATLEPGDLFVQR
                     HRIPLAADATPGAMRVSLGAYHPDTGSRLRGQAGDRGVDALVLGSYNLLQ"
     gene            842..1606
                     /locus_tag="AC162G_11645"
     CDS             842..1606
                     /locus_tag="AC162G_11645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5076518.1"
                     /translation="MPDHPYDLSLVVACYNEEVELEESIRQVVEILDSTRWTYEIIFV
                     DDCSQDRTREIIEELLAKYKGRNFRKLFHEKNTGRGRTVADGVRLARGDVAGYIDIDL
                     EVHARYIPSMMIAIQNGADLATAHRIYKVQLGLFNRFVLSTGYAWLVRHLLGVHLKDT
                     ETGYKFFKREKIVPVLDEIEDERWFWDTEVMVRSLLRGYRIEEIPCLFVRRYDRQSTV
                     NVVRDSLDYLVKLWRFRRVVRQLRAQPQAERTPDVL"
     gene            1635..2255
                     /locus_tag="AC162G_11650"
     CDS             1635..2255
                     /locus_tag="AC162G_11650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5076519.1"
                     /translation="MSGFSSLLYSNRLAYHLVMRVLYGRHFDARYEAVAAEIPSGASV
                     VDVCAGDAYLYLKCLRHKPVRYLALDISPAMVAWANRHGIPARQFNLWQDTPPPGDIV
                     VMQASLYQFLPHADAIVRKLLAAAHQKVIIAEPVRNLSASSHPWLSFVSRRLSAPDST
                     GGRYTGQRFDRQSLTALFHAFDAFEREFIVPGGREMIGIFRGQCDG"
     gene            2248..>2520
                     /locus_tag="AC162G_11655"
     CDS             2248..>2520
                     /locus_tag="AC162G_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR5076520.1"
                     /translation="MVDAFRQRTLSVMESSRIPFNRPSFEGNEQVYIAEATASGHLSG
                     DGPFSRKCHAFLEAALGAPKALLTTSCTHALEMCALLLDIQPGDEVI"
BASE COUNT          432 a          816 c          778 g          494 t
ORIGIN      
        1 cgcaggccgc gcgcgcgctc gacgccggcc cgtcgacgct gccggtcgcc gcttcgggat
       61 tgagcatcca ggacctcgat ccgcagacgt tcgacctgct gctcaggcga cgcgatttca
      121 aagtgaagtg gttcgactgc cgcagcgcga ttctcttccc cgccggcggc gcgccggcgc
      181 gttacctgga gccggatttc ttctcatgtg atcccgacct gatgtcgcat tatttgccag
      241 gcgcacccat catcgctcaa ccgcgctggc ccgattccgg caacgtgatc ttcacactgc
      301 gccagttcga tgcgcgcgcg ctgctcgacg gcgccctgcc gcgcattgcg gcgcagccga
      361 tctacatcgg cagcgagagc ttcgacgcgg ccgatcccgc cggcgggctt gagccgctgg
      421 cgctgccgat cgacctgagt ggattgcagt tattggggtg ggagatggag cgggatacgt
      481 ggaagccggg tgagacaatc cagatcagga cctattggaa ggtcacggcg gcgcttgcgc
      541 cgcccttgtc gctattcgtg cacgtcacgc ggcccgacgg atcgatcttc gctcagcacg
      601 atggactgga catcggcgtg gcgacgttgg agccgggtga cctcttcgtg cagcggcacc
      661 gcattccgct cgcggcggac gccacaccgg gcgcgatgcg tgtctcgctc ggcgcgtacc
      721 atcccgacac aggcagccgt ttgagagggc aggccggcga tcgcggggtg gatgcactcg
      781 tcctcggttc gtataatctg ctacaatgaa gcgccgccgt caagcgataa tggaggcacg
      841 gatgccagac catccttacg atttgagcct ggtcgttgcg tgttacaacg aagaggtcga
      901 gcttgaagag agtatccggc aggtcgtcga aatactcgat agcacgcgct ggacttacga
      961 gatcatcttc gtcgacgact gcagccagga tcgcacgcga gaaatcatcg aagagcttct
     1021 ggcgaaatat aagggcagga atttccgcaa gctgtttcac gagaagaaca cggggcgcgg
     1081 ccgcaccgtc gccgacggcg tgcggctcgc gcgcggcgac gtcgccggct acatcgacat
     1141 cgacctggaa gtccacgccc gttacattcc ctccatgatg atcgccatcc aaaatggcgc
     1201 cgacctggct accgcgcatc ggatttacaa ggttcagctc ggactgttca atcgtttcgt
     1261 cttgagcacc ggctatgcct ggctggtgcg tcacctgctc ggcgttcacc tgaaagacac
     1321 ggaaaccggt tataagttct ttaagcggga aaagatcgtg ccggtgctcg acgagatcga
     1381 agacgagcgc tggttctggg ataccgaggt catggtccgt tccttgctgc gcggctaccg
     1441 gatcgaagaa atcccgtgcc tgtttgtccg gcgctatgat cggcagtcca cggtcaacgt
     1501 cgtgcgcgat tcgctggact acctggtgaa gctgtggcgc ttccgccgcg tggtcaggca
     1561 gttgcgcgcg cagccgcaag ccgagcgcac gcccgacgtg ttataatccg cgcgcccggc
     1621 caagtgcttc gacggtgtca ggcttctctt cgctgctcta ctcgaatcga ctggcgtacc
     1681 atctcgtcat gcgcgtgctg tacggtcgcc acttcgacgc gcgctacgag gcggtggccg
     1741 ccgagattcc gtccggcgcg tcggttgtcg atgtatgcgc gggcgacgct tacctgtatc
     1801 tcaagtgcct gcgccacaag ccggtgcgct atctcgcgct cgacatctcg cccgcgatgg
     1861 tcgcgtgggc gaaccggcac ggcatcccgg cgcggcagtt caacctgtgg caggacacgc
     1921 cgccgcccgg cgacatcgtc gtcatgcagg ccagcctgta ccagtttctg ccgcacgccg
     1981 atgcgatcgt gcgcaagctg ctggccgctg cgcaccaaaa agtgatcatt gccgagccgg
     2041 ttcgaaacct ctcggcttcg tctcacccgt ggctgtcctt tgtcagccgc cgcctgagcg
     2101 cgcccgattc gaccggtgga cgttacacgg gccagcgctt cgatcgccaa tcgctcaccg
     2161 cgctctttca tgcgttcgac gcgttcgagc gtgagttcat cgtccccggc ggccgggaga
     2221 tgatcggtat cttcagagga caatgcgatg gttgatgcgt ttcgccagcg caccctctct
     2281 gtcatggaat cgtctcgcat cccctttaat cgccccagct tcgagggcaa cgagcaagtc
     2341 tacatcgccg aagcgaccgc cagcggtcac ctatccggtg acggcccgtt ctcgcgtaag
     2401 tgccacgctt tcctcgaagc cgcgctgggc gcgcccaagg cgctcctgac cacatcgtgc
     2461 acgcacgcgc tggagatgtg cgccctcctg ctcgacattc agcccggaga cgaagtgatc
//
LOCUS       DBNHXB010000291         2977 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_76844, whole genome shotgun sequence.
ACCESSION   DBNHXB010000291 DBNHXB010000000
VERSION     DBNHXB010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_76844"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="AC162G_11660"
     CDS             <1..269
                     /locus_tag="AC162G_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5076521.1"
                     /translation="KVDSQVIELSPIEFRLLVTLIRNEGKVLTHQFLLEQVWGPTYKE
                     ERHYLKLYIWYLRQKLEKDPNHPLLFLTERGVGYRLAREGESEV"
     gene            complement(293..1963)
                     /locus_tag="AC162G_11665"
     CDS             complement(293..1963)
                     /locus_tag="AC162G_11665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR5076522.1"
                     /translation="MIRTATALLTLAALACACALSDPTPAPTTPPLPTEAPTATPEPT
                     LSPTPTPVPIGVYVAPWLPSGLKAHLDDTLAALPNDFFPVPDASGASVRVGYSAPAGL
                     PALGRQVFGLVAAFPTVADEATWTEVEASWKGAPGAQHSPLRMTPNTYAALSTRLGEA
                     APGAVEVLEADRIADALWADRDAWGFVPFDELDPRLKVLYVDGRSPLRRNLDLAAYPL
                     VTDVVLSGDLNEAAKLQAAFGEAYTNRDESRMTILGMTGVTALTRDLAWMMEQNGVLW
                     PAEHIRPWLEEVDILHVSNEVSFTPNCPAPILGTGSVVFCSDPKYFDLITSLGVDVIE
                     NTGNHLNDWGSMPFSYTLDLYVQNNVPYFGGGYNLADAARPITIEDHGNLIGFVGCNP
                     NGPDIDWATDSSPGSYPCREGGGIDFSRVKPQIDELRAQGALPVLTIQYWEFYTYEPT
                     YQQALEFRQAAELGAAIVSGSQAHHPQGFDFASGAVIHWGLGNLFFDQSATAGQRQGL
                     FDRYVIYDNRVLSLEVLTFIREHYAQPRPLTPAERADLLSVLFAASGW"
     gene            complement(2087..>2977)
                     /locus_tag="AC162G_11670"
     CDS             complement(2087..>2977)
                     /locus_tag="AC162G_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076523.1"
                     /translation="TDDWSGRPAERAMGALAHVLSGLPLSNSTRASLAVAFSRLLGDP
                     YDFVNAPVTQLFQEHVLGSTMFEEVYRRRPRFDLPSLLFDEDRAALSASARATLLDLM
                     SRAGVRVCVYTSRPSLPPTDVLDWLGNADRAPVGYSPEAELALRLVGLTDLPLIGMGR
                     MQWLARRVGSKVEYLTKPAPVQALAAILSAISCRESESLEVAYRFVSEGESVPLMSRM
                     FEQPVDLWVVEDATPGLHAAAGAVDLLGKHGVSVRLRALGVSSGGPKAEALAGPCEVI
                     VPTVNEAIAYIAEQIQAARP"
BASE COUNT          597 a          913 c          961 g          506 t
ORIGIN      
        1 tcaaggtcga cagccaggtg atcgagctgt cgcctatcga gttccggcta ctcgtgacac
       61 tgattcgaaa cgaaggcaag gtgctcacgc atcagttctt gctcgagcaa gtctggggtc
      121 ccacgtataa agaagaaaga cattacctca aactgtatat ttggtatctg cgccagaagc
      181 tcgaaaagga tcccaaccac cccctgctgt tcctcaccga gcgtggcgtc ggctaccggc
      241 tggcgcgcga aggcgaatca gaagtttgag tcgggtttgt gcaacctccg agtcaccacc
      301 cgctcgccgc gaacagtacg gacagcaagt cggcgcgctc ggctggtgtg agcggccgtg
      361 gctgagcgta atgctcgcgg atgaaggtca acacctccag gctgagcacc cgattgtcgt
      421 aaatcacata ccgatcgaac aatccctggc gctgacccgc cgtcgcagac tgatcgaaaa
      481 acaggttgcc caggccccag tgaatcaccg cgccactcgc aaagtcgaag ccctgcggat
      541 ggtgagcctg gctgccactg acgatcgccg cgccaagctc cgccgcctgg cgaaactcca
      601 gcgcctgctg ataggtaggc tcgtaggtgt aaaactccca gtattggatg gtgaggactg
      661 gcagcgcgcc ttgcgcgcgt agttcgtcga tctgcggctt gaccctgctg aaatcgatcc
      721 cgccgccctc gcgacaagga tacgagcctg gcgacgaatc agtggcccag tcgatatcgg
      781 ggccgttggg attgcagccg acgaaaccga tcaggttgcc gtgatcttcg atggtgatcg
      841 gtcgagccgc gtcggctaga ttgtagccgc cgccgaaata tgggacgttg ttctgcacat
      901 agagatcgag cgtgtacgag aagggcatgc tgccccaatc gttcaggtgg ttgcccgtat
      961 tctcaatcac gtccacaccc aggctggtga tcaggtcgaa atacttgggg tcggagcaga
     1021 acacaaccga gcctgtgccg agaatcggcg ccggacagtt cggggtgaac gacacttcgt
     1081 tgctgacgtg aagaatatcg acttcttcca gccaggggcg gatgtgctcc gccggccaca
     1141 gcacgccgtt ctgctccatc atccacgcca gatcgcgagt gagcgcggtg acgcccgtca
     1201 tgcccaggat cgtcatacgc gactcgtcgc gattggtgta cgcctcaccg aatgccgctt
     1261 gcagcttcgc cgcctcgttc aggtcgccgc tcaacacgac gtccgtaacc aatggataag
     1321 cagccaggtc gaggttgcgc cgcagcggcg agcggccgtc gacgtacaac accttcagac
     1381 gcgggtcaag ctcgtcgaag gggacgaatc cccacgcatc gcggtcagcc cacagtgcat
     1441 cggcgatccg atcggcctca agcacttcga ccgcgccggg cgctgcctca cccaggcgcg
     1501 tactgagcgc agcgtaagtg ttcggcgtca tgcggagcgg gctgtgctgc gcgccaggcg
     1561 ctcccttcca agatgcttcc acctcagtcc aggtggcttc gtcggcgact gtcggaaatg
     1621 cggcgaccag gccgaacacc tgccggccaa gcgctggcaa accggcggga gcgctgtagc
     1681 cgactctcac gctcgcgccg gacgcgtctg gaacagggaa gaaatcattc ggaagcgcag
     1741 ctaacgtgtc gtccaggtgc gccttgagac cagaagggag ccatggggcg acgtacacgc
     1801 caatcggaac cggcgtcggc gtcggcgaga gtgtcggttc gggtgtagcg gtcggcgctt
     1861 cggtcggaag gggtggagtc gtcggggcgg gcgttgggtc gctcaatgca cacgcgcagg
     1921 cgagagcggc gagtgtgagg agggcagtcg cggtgcgtat cataatcgct caacgtcgtg
     1981 tgggacggct cgacattgta gcatcgcggc ggatttgtga caaggacggg gaggtagtgg
     2041 aggagcgtga tgggtaattg gggattggta atcggggctt gggaggttat ggtcgggcag
     2101 cctgaatctg ttcggcgatg taggcgatcg cttcgttgac ggtaggcacg atgacttcgc
     2161 aaggcccggc gagcgcctcg gctttcggcc cgccggaaga tacgccgagc gcgcgcagcc
     2221 gcacgctgac gccgtgcttc cccagcagat cgaccgcgcc cgcagccgcg tgcaggccgg
     2281 gcgtcgcatc ttccacgacc cacaggtcga ccggctgctc gaacatgcgc gacatgagcg
     2341 gcaccgattc accttcggat acgaaccggt acgccacctc cagagactcg gattcgcggc
     2401 aggatatggc gctcaggata gcagccagtg cctgcactgg cgcaggcttg gtgaggtact
     2461 ccaccttcga gccgacgcgg cgcgccagcc actgcatacg ccccattcca atcaggggaa
     2521 gatcggtcag gccgacgagc cgcagcgcaa gctcggcctc cggagaatag ccgaccggcg
     2581 cgcgatcggc attgcccagc caatccagca cgtcggtggg cgggaggctg ggacgtgacg
     2641 tgtaaacgca aacccgcacg ccggcgcgcg acatcaaatc cagaagcgtc gcccgcgccg
     2701 aagcgctcaa cgcagcccga tcttcgtcga acagcagcga aggcagatcg aagcgcgggc
     2761 gcctgcggta aacttcctcg aacatcgtcg agcctaagac gtgctcttga aacaactgtg
     2821 tgaccggcgc attcacgaag tcgtaagggt cgcccagcag cctggaaaag gccacggcca
     2881 agcttgcacg cgtggaattg gagaggggca agccgctcaa cacgtgtgcc agtgcgccca
     2941 tcgcgcgctc ggcaggccgg ccggaccagt cgtccgt
//
LOCUS       DBNHXB010000292         3364 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1922428, whole genome shotgun sequence.
ACCESSION   DBNHXB010000292 DBNHXB010000000
VERSION     DBNHXB010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1922428"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..801
                     /locus_tag="AC162G_11675"
     CDS             205..801
                     /locus_tag="AC162G_11675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5076524.1"
                     /translation="MPVVHEDGQLVARIREGDLSALGALYDKYRLQVFHTALAVTRDR
                     QAAEDILQEAFLKVHAYANRIDLGVPLAPWLYRVTVNLSYTWATRQAKWRTSLETLID
                     RLVAPPRVAPEGHFEARDLQTRIQRAVDALPFNQRVVIILYYLSDLSLKEIAYILDCP
                     VGTAKSRLHYGREALRRQLAQSLEPQAALVLEMSYDFT"
     gene            788..1075
                     /locus_tag="AC162G_11680"
     CDS             788..1075
                     /locus_tag="AC162G_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076525.1"
                     /translation="MTSRERPNVAGDGLGDLLRTALKDGVRAVTPSAGARQALLRAAA
                     ERQRAAIPYSFERIQDRRDEEWRPWEARTATESPTAALLLHARLIALRIVQ"
     gene            1234..1704
                     /locus_tag="AC162G_11685"
     CDS             1234..1704
                     /locus_tag="AC162G_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076526.1"
                     /translation="MTRSALHHWILRAIILLLVVALLAACSRGGTPAAGGAGTAAQTG
                     VPGTGGTAPAPAATEEVLPPDIPIIEDTPIERASEGTLEYRVPMPLQDVLDYYQTELP
                     AQGWEETGRPNILGTVATLRYSKEVEGKTRGLSVSMQYNENAQSTLVRLFWQGY"
     gene            1911..>3364
                     /locus_tag="AC162G_11690"
     CDS             1911..>3364
                     /locus_tag="AC162G_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5076527.1"
                     /translation="MHHKRLMMIASLLVLASLLLTACPAPAATPETVVVTAPPEVQVV
                     TATPPQPPPAGPKTLVICQGQEPDSLYSYGTDMLAAAHIQEAIYDGGDWQPAGIDSRT
                     YDFQPVILEKMPSVDDGDAVINAVTVGEGDTVLNDAGEVVELAAGETIKPAGCLSSDC
                     AVTYEGGDVEMDQFVVTFKLLPGLTWSDGEPLTADDSVYGFELLVDPDTPESKFTTDR
                     TTSYEATDDVTAVWTGLPGFVDATYFLNFFQPFPRHLWQEQLGYGAADLVTAEESSRT
                     PLGWGPFVITEWVPGDHITAEKNPNYFRAAEGLPKVDTIVFRFTGEDPNPVIAAALAG
                     ECDIIDQSSGLESQGQLLIELEASGQLVPTFVTGTAWEHVDFGINVVDSYERPDFFED
                     VRFRQAFAQCLGRQEVVDALLFGRSTVIHNFIPPEHPMYAEDVTQWPDDPAAGMALLD
                     EIGWVDDDGDPATPRVAQGVEGVPDGTALSFKWQ"
BASE COUNT          619 a         1079 c         1069 g          597 t
ORIGIN      
        1 tgagagtcgc gccatggatc gaacagaaca tttgaccgaa gccgatctgg aacatgaacg
       61 ctggattgta cttgcgttta gtggcactta tctattataa tcaccagtaa atccgcacgc
      121 ccgcacattg ccccagaatt gaaccttttc gaccaccggg tcgtattaaa gaagaacggg
      181 gcattctaag gctgagtgaa aattatgccg gtcgttcacg aagacggaca actggtcgct
      241 cgtatccgcg aaggagacct gtcggcgctc ggcgcgctgt atgacaaata tcgcctccaa
      301 gtgttccata cggccctggc cgtcacgcgc gatcggcagg cggccgaaga catcttgcaa
      361 gaagccttcc tcaaagtgca tgcttatgca aaccgcatcg acctcggagt tccgctggcg
      421 ccctggctgt accgcgtcac ggtgaacttg agttatacct gggccacgcg ccaggcgaag
      481 tggcgcacct cgctggaaac tttgatcgat cgtctcgtcg cgccgccgcg cgtggcgccg
      541 gagggccact tcgaggcgcg cgacctccag acccgcatcc aacgcgccgt cgacgcgctg
      601 ccgttcaacc agcgcgttgt gatcatcctc tactatctga gcgacctcag cttgaaggag
      661 atcgcttaca ttttggattg tccggtcggc acggccaagt cgcgcctgca ctatgggcgc
      721 gaggcgttgc gccgccagtt ggcccagagc ctggagccgc aggctgcgct tgttttggag
      781 atgtcctatg acttcacgtg agcgcccgaa tgtcgcgggc gacggcctcg gcgacctgct
      841 gcgcacggcg ctgaaggacg gcgtccgcgc cgtgacaccg tccgctggtg cgcgccaggc
      901 gctcctgcgc gcggcagccg aacggcagcg cgccgcgatc ccgtactctt ttgagcggat
      961 ccaagatcgg cgggacgaag agtggcgccc atgggaagcg cgcacggcaa ccgaatcacc
     1021 taccgcggcg ctgttgctgc acgcgcggct gattgcgctg cgcatcgttc agtagggcag
     1081 gtttctatac ctgcccttga atggcggcta tggaaagccg ccctacattg gggtcgagcc
     1141 tttaggcgga tagttctggt ggtcaagacc ggctaaagcc tcgactccga cccaagatgc
     1201 ggaacttgcc aaagtccagc agggggagga ttcgtgacgc ggagcgcgct tcatcactgg
     1261 atactcagag caatcattct tttgctggtc gtggcgctcc tggcggcctg cagtcgaggt
     1321 ggcacacccg cagccggagg cgcgggtacc gctgctcaga cgggtgtgcc gggcacgggc
     1381 ggcaccgcgc cggcgccagc ggcgacagag gaggtgttgc cgcccgacat cccgatcatc
     1441 gaggacaccc cgatcgagcg cgccagcgaa ggcacgctgg aataccgcgt gccgatgccg
     1501 ctgcaggacg tcctcgatta ttatcagacg gaacttcctg cgcagggatg ggaggagacc
     1561 ggccggccga atattcttgg cacggtcgcg acgctgcgct actcgaagga agtcgaaggc
     1621 aagacccgtg gcctgtctgt cagcatgcaa tacaacgaga acgcgcagtc cacgctggtg
     1681 cgtttgttct ggcaagggta ttagtttctt ctcgatcggc gcaggtctcc gaccgcgccg
     1741 ggtgtcgacc gcaggtctcc agggccttgg tcaggagacc ttcagccatc ctggctacag
     1801 gggttgaaag gaggtgatga ccgagagttg agatgtcact cactgcctgt gaaggcgaag
     1861 tctgctcgta tcgcgcaagc aacaacacca tctttggagg agatgaagac atgcatcaca
     1921 agcgtctaat gatgatcgcc agcctgctgg tcctggcatc cctgctgctg acggcctgcc
     1981 ccgctccggc ggcgacaccg gagactgtcg tcgtgacggc tccgccggaa gtgcaggtag
     2041 tcaccgcgac gccgccgcag ccgccaccgg ccgggcctaa gacgctggtc atctgccagg
     2101 gccaggagcc ggattcgttg tactcctatg gcactgacat gctggccgcc gctcacatcc
     2161 aggaggccat ctacgacggc ggtgactggc aacctgctgg tatcgatagc cgcacctacg
     2221 acttccagcc ggtcatcctg gagaagatgc ccagcgtcga cgacggcgac gccgtgatca
     2281 acgccgtcac ggtcggcgaa ggcgacacgg tgctcaacga cgccggcgaa gtcgtcgagc
     2341 tggcggccgg cgagacgatc aagccagcgg gctgcctgag cagcgactgc gctgtgactt
     2401 acgaaggcgg cgacgtagag atggaccagt tcgtcgtcac cttcaagctg ctgcccggcc
     2461 tcacctggtc ggacggcgag ccgctgaccg ccgacgactc ggtctacggc ttcgagctgc
     2521 tggtcgatcc cgacacgccg gagagcaagt tcacgaccga ccgcaccacc tcctacgagg
     2581 cgacggacga cgtgacggcg gtgtggaccg gtctgcccgg cttcgtcgac gccacctact
     2641 tcctcaactt cttccagccg ttcccgcgcc acctgtggca ggagcagttg ggctatggtg
     2701 ctgccgacct ggtcaccgcc gaagagtcct cccgcacgcc gctgggctgg ggcccgttcg
     2761 tcatcacgga gtgggtcccc ggcgatcaca tcaccgccga gaagaacccc aactacttcc
     2821 gcgctgccga aggcctgccc aaggtcgaca cgatcgtctt ccgcttcacc ggcgaggatc
     2881 cgaacccggt gatcgccgcc gcgttggccg gcgagtgcga catcatcgac cagtccagcg
     2941 gcctggaatc gcagggccaa ctgctgatcg agcttgaggc gagcggccag ttggtcccga
     3001 ccttcgtgac cggcacggcc tgggagcacg tcgacttcgg catcaacgtc gtcgactcgt
     3061 acgagcgtcc cgacttcttc gaggacgttc gcttccgcca ggcattcgcg cagtgcctgg
     3121 gccgccagga agtcgtcgat gccctgctgt tcggccgctc gaccgtgatc cacaacttca
     3181 tcccgcccga gcaccccatg tacgccgagg acgtcacgca gtggccggat gatccggcgg
     3241 cgggcatggc gctgctggac gagatcggct gggtggacga cgacggcgat ccggccacgc
     3301 cgcgcgtggc gcagggtgtg gaaggcgtcc ccgacggcac tgccctgtcg ttcaagtggc
     3361 agag
//
LOCUS       DBNHXB010000293         8323 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_76947, whole genome shotgun sequence.
ACCESSION   DBNHXB010000293 DBNHXB010000000
VERSION     DBNHXB010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_76947"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1798)
                     /gene="groL"
                     /locus_tag="AC162G_11695"
     CDS             complement(158..1798)
                     /gene="groL"
                     /locus_tag="AC162G_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431940.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR5076528.1"
                     /translation="MAKQLTFGEDARRSLKAGMDTLAIAVATTLGPKGRNVALDKKFG
                     APTITHDGVTVAKEIELSDPYENMGAQLLKEAATKTNDVAGDGTTTATVLAHSMVTEG
                     LKMVAAGANPMLLKRGIEQAREAIVEAIKKMSIDVKTRDDIAHIATISAADEEIGELI
                     AEVMDKVGKDGVITVEESKGLAFETEYVEGMQFDRGYISPYFITSVESMEAVLENPYI
                     LIHDKKISAASDMVPLLEKLIQRGKRELVVIAEDVDGEALATLVLNKLRGVLNMLAIK
                     APGFGDRRKAMLQDIAVLTGGTVITEELGRKLESVTLDDLGQAAKVTADKDNTTIIEG
                     KGTTKEIKGRIEQIKVEIENTTSDYDKEKLQERLAKLAGGVAIIRVGAGTEVELKEKK
                     HRVEDALSAARAGVEEGMVPGGGVALINAMPALDKVKPELDDEVTGVAIVRRALEEPM
                     RRLAHNAGMDGAVVIDNVRRQQKETKNKNIAYNVMTGEYEDMIKGGIMDPAKVTRSAV
                     ENATSIAAMILTTEALIADKPEKKEAALMPPGGGMGDF"
     gene            complement(1811..2116)
                     /gene="groES"
                     /locus_tag="AC162G_11700"
     CDS             complement(1811..2116)
                     /gene="groES"
                     /locus_tag="AC162G_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR5076529.1"
                     /translation="MAKTKLNLKPLGDRVVVEPLEKEEKTTSGIILPETAKEKPQEGE
                     VLAVGPGRTDDDGKRVPMDVKKGDIVLFAKYAGTEIKIDDKKLLILKESDILAIVEK"
     gene            complement(2308..2460)
                     /locus_tag="AC162G_11705"
                     /pseudo
     CDS             complement(2308..2460)
                     /locus_tag="AC162G_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
     gene            complement(2457..2816)
                     /locus_tag="AC162G_11710"
     CDS             complement(2457..2816)
                     /locus_tag="AC162G_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010888345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5661 family protein"
                     /protein_id="HIR5076530.1"
                     /translation="MSLRARYIVSNKRRISAEEVQQVGATLGLDWQKIDLEQFRRGLE
                     VELERGVRDPETNVTNDDLALTGNIAWAHLKEFPDYYTRLDQLEARRTSIRRRAADAH
                     HRLRSRPIRYDKNRASP"
     gene            complement(2970..3338)
                     /locus_tag="AC162G_11715"
     CDS             complement(2970..3338)
                     /locus_tag="AC162G_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5076531.1"
                     /translation="MTRVYLADAQRAERSALRLLLLDLNMEVVGEAADWPDTLAQAPA
                     THPDMLLVDWDLLANRSSADLAELRVACAAAVVIVLISHLDARQQAALSAGADAFISK
                     GEAPDRVVERLRAAAESVGS"
     gene            complement(3415..3642)
                     /locus_tag="AC162G_11720"
     CDS             complement(3415..3642)
                     /locus_tag="AC162G_11720"
                     /inference="COORDINATES: protein motif:HMM:NF014090.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076532.1"
                     /translation="MSDGLTVPPCTRGRTPLASSTAIVLTGSLAEIAAGSIAMGLSGY
                     LAAWRGQRISGFSQTLMLSGCRLYSAKLQTK"
     gene            complement(3720..3887)
                     /locus_tag="AC162G_11725"
     CDS             complement(3720..3887)
                     /locus_tag="AC162G_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076533.1"
                     /translation="MADKTSKTKPKRLSKGQRTHVRRLKQAARKTGIAHSPPTSASRP
                     SSVPKKEDQHN"
     gene            complement(4131..4412)
                     /locus_tag="AC162G_11730"
     CDS             complement(4131..4412)
                     /locus_tag="AC162G_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5661 family protein"
                     /protein_id="HIR5076534.1"
                     /translation="MSNKRRISAEEAQQVGATLGLDWQKIDLEQFRRGLEVELEHGAR
                     DPETNVTNDDLPLTGKIAWAHLKEFPDYYTRLDKLEAEADEFWTARLKG"
     gene            complement(4611..5453)
                     /locus_tag="AC162G_11735"
     CDS             complement(4611..5453)
                     /locus_tag="AC162G_11735"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015192377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR5076535.1"
                     /translation="MFGQRNSGGRGSSILPRLVIGAVIAIISLIGYFGAQVYNPATDE
                     VQHIDMTVEQEIALGLQAAPEIAAQFGGLDPDEQAQALVDRVGQQLVAQTAATDTDYP
                     FQFYLLADPQTINAFALPGGPVFITRGLLDRLETEGQLAGVLAHEVGHVVARHSAEHI
                     AKARLTEGLTGAAVIAAYDPNDPSSQQTAQVAVLIGQLINLKFGRDDELESDRLGVRF
                     MAESGYDPRSMMQVMEILAEASQGQQPPEFFSTHPNPENRIERIQAAIEEAFPTGVPE
                     GLIQ"
     gene            complement(5843..6052)
                     /locus_tag="AC162G_11740"
     CDS             complement(5843..6052)
                     /locus_tag="AC162G_11740"
                     /inference="COORDINATES: protein motif:HMM:NF016141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076536.1"
                     /translation="MGVVGAFVAGWFLTPLFWDQHNQPGQLQRAGFAGVVGGRHPPTG
                     GGRQSRPPGRAVRTLTVFHSVQQTP"
     gene            complement(6112..6468)
                     /locus_tag="AC162G_11745"
     CDS             complement(6112..6468)
                     /locus_tag="AC162G_11745"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR5076537.1"
                     /translation="MSERERRNFRASQLIWLLVGVLEVLIALRIGLKLIAATPDNPFA
                     AFIYNLTNIFVFPFAGLTPTPAVGSGVLELHSIIAMVVYALAGWAIARLVWVIFYRRR
                     EPVDVTQTTIQDDRTE"
     gene            complement(6695..7912)
                     /locus_tag="AC162G_11750"
     CDS             complement(6695..7912)
                     /locus_tag="AC162G_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076538.1"
                     /translation="MALTIAACLANGRFVTSDPALDNSAASQKARADYAMQQAQQQEA
                     NAHAAAVNETAQAQAADGVLALAATATAQALGVARVQLAMTADAANFPSTQSAVFAQQ
                     TAQAIEAKATIDARAMLLAQEQAQATATAQAMSAQQTAQAIEAKATIDARAMLLAQEQ
                     AQATATAQAMQREGVTADQEASTSRRQSELMSWLIPIVAAIAFGLVLLLGAKFIGGMI
                     DAANERRSLENQRLALLGTLFEAPTETIVFADDPQTGFATPQLLNTPGNGKGDDTSGS
                     LASHTPMIDTEAPPVIVILSNGDKRLADRAEAREEAARCKLAMKLLRDAINHVGAQSN
                     RIPPAAQLGWPAGAWTIAVAILRPYGVEILPGMDGGTYLVGQYPTLQALYIAIGERRL
                     TLYPPSAESVTNG"
BASE COUNT         1458 a         2553 c         2427 g         1885 t
ORIGIN      
        1 acgcgcgcag cagttgtggc gcgtcgcgca gtttccacat gccgatatct ttcctgtgct
       61 caatgcgcgg cgcggccaac tcgacgagcg cgctcaacaa agaaacccgg ctccctttcg
      121 ggaaaccggg tttccaaaca ttggcctgct ttgacgatta gaagtcaccc atgccaccac
      181 cgggcggcat cagggcagct tccttcttct cgggcttgtc ggcaattaga gcttcggtcg
      241 tgaggatcat cgccgcgatg ctggtggcgt tctccaccgc gctgcgcgtg accttggcgg
      301 ggtccatgat gccgcccttg atcatgtcct cgtactcgcc ggtcatgacg ttgtaggcaa
      361 tgttcttgtt cttggtttcc ttctgctggc ggcgaacatt gtcgatgacc accgcgccgt
      421 ccatgccggc attgtgagcg agccggcgca tcggctcttc gagcgcgcgg cgaacgatcg
      481 ccacaccggt gacctcgtcg tcgagctcgg gcttcacctt atccagcgcc ggcatggcgt
      541 tgatcagagc cacaccgccg ccaggcacca tgccctcttc gacgcccgcg cgggccgccg
      601 acagcgcgtc ctcgacgcga tgcttctttt ccttcaactc cacctcggtg ccggcgccga
      661 cgcgaatgat ggccacgccg ccagccagct tggccaagcg ttcctgtagc ttctctttgt
      721 catagtccga agtggtgttc tcgatctcga ccttgatctg ctcgatgcgc cccttgattt
      781 ccttggtcgt gcctttgcct tcgatgatgg tcgtgttgtc cttgtcggca gtcaccttgg
      841 cggcctgacc cagatcgtcg agcgtgacgc tctccagctt gcggcccagc tcctcggtga
      901 tgacggtgcc gccggtcagc acggcgatgt cctgcagcat cgccttgcgg cggtcgccga
      961 agccaggggc cttgatggcc agcatgttca gcacgccgcg cagcttgttg agcacgagcg
     1021 tcgccagcgc ctcgccgtcg acgtcctcgg cgatgacgac cagctcgcgc ttgccgcgct
     1081 ggatcagctt ctccagcagc ggcaccatgt ctgaagcggc ggagatcttc ttgtcatgga
     1141 tcaggatgta cggattctcg agaacggctt ccatcgactc gacgctggta atgaagtacg
     1201 ggctgatgta gccacggtcg aactgcatgc cctcgacgta ctcggtctcg aaggccagac
     1261 ccttgctctc ttcgacggtg atcacgccgt ctttgccgac cttgtccatc acctcggcga
     1321 tcagctcgcc gatttcctcg tcggcggcgg agatcgttgc gatgtgggca atgtcgtccc
     1381 tggtcttgac gtcgatcgac atcttcttga tcgcttcgac gatcgcctcg cgggcctgct
     1441 cgatgccgcg cttgagcagc atcgggttgg cgccggcggc gaccattttc agaccctcgg
     1501 tgaccatgga gtgcgccagc acggtggcgg tagtggtgcc atcgccagcc acatcgttcg
     1561 tcttggtggc ggcttccttg aggagctgcg cgcccatgtt ctcgtaagga tcgctcagct
     1621 cgatctcctt ggcgacggtc acgccgtcgt gggtgatggt cggggcgccg aacttcttgt
     1681 cgagcgcgac gttgcggccc ttggggccga gtgtggtggc cacggcgatc gccagcgtat
     1741 ccatgcccgc cttcaacgag cgccgcgcgt cttcgccgaa tgtcagttgc ttagccatgt
     1801 gtctctccct ttacttctcg acgattgcga gaatgtcgga ttctttgagg atgaggagct
     1861 tcttgtcgtc gatcttgatt tcggtgccgg cgtatttggc aaacagcacg atgtctccct
     1921 tcttcacgtc catcgggacg cgcttgccgt cgtcgtcggt gcggcccggc cctacggcca
     1981 gcacttcgcc ctcctgcggc ttctctttcg ccgtctcggg tagaataatc ccgctggtgg
     2041 tcttctcttc cttttcgagg ggttcgacga ccacgcgatc gccaagcggc tttaagttca
     2101 acttcgtctt agccatgttg cagacctccc ataggtatat agtattgggt ttctcgacca
     2161 ttcgtcttgg ccgggtcgat acatcaggac tggttagcac tctatccgcg agagtgctaa
     2221 ccctatcgag aaggggcaat gtgccagctc gggtctaagc ctggccttca gcccgccgaa
     2281 ttatggagtc gggacatcgc tctacttctc gtcgatcgcc tgcctctcga tcgccgtgta
     2341 gagcttttcg tatccccagt tagcgcacat ctcggagtgc accatatact ggctaatcgc
     2401 agtgagttca gcggccaaga gttggttgag taccgcgagt agcttgtcgt tgcctttcat
     2461 ggtgatgctc gattcttgtc gtatctaatc ggcctcgatc tcagccggtg gtgggcgtca
     2521 gcagcgcgac gcctaataga cgtccgcctt gcctcgagct ggtccagacg cgtgtagtag
     2581 tccgggaatt ccttgagatg tgcccaggcg atattccccg taagagccag gtcatcgttc
     2641 gtgacgttcg tctccgggtc ccgcacacca cgctccaact caacttcaag gccgcggcgg
     2701 aactgctcga gatcgatctt ctgccagtct aggccaagtg tggcgccgac ttgctggact
     2761 tcttctgcgc taatccgacg tttgttgctc acgatgtacc tcgctctcaa tgacatgttt
     2821 ctacccagtg cgcggcccgg gatgaatccc agtgcgcggc ccaaccttcg gataagctgc
     2881 ccagttcacg ccggcgctcc gcggagcgca gcgtagtgcg gccgattttc agaccgctcc
     2941 tccgccttga gacccgcctg gtgatgtcat caagagccaa cgctctcggc cgcggctcgc
     3001 aggcgttcca ccacacggtc gggtgcctca cctttgctga taaaggcatc ggccccggcg
     3061 gagagcgccg cctgctgacg ggcatccaga tggctgatga ggacaatgac gaccgcggcc
     3121 gcgcaggcca cgcgcagttc ggccagatcc gcgctggacc gattagcgag taagtcccaa
     3181 tccaccagca acatgtctgg gtgggttgct ggagcctggg ccaacgtgtc aggccagtcg
     3241 gcggcttcgc cgactacttc catattgaga tccagcagta acagacgtag ggccgaacgc
     3301 tctgcgcgct gagcatcagc cagatagaca cgggtcatga attcatgcta caacgaatac
     3361 gccccagcca catccatcgg ccggggagta tttgactccg ccagttgggg gagattattt
     3421 ggtttgaagc ttcgcggaat ataagcggca tccactcaac atcaatgtct ggctgaaccc
     3481 acttattcgc tggcccctcc aagccgcgag gtaaccactc aagcccatag cgatagaacc
     3541 cgccgcgatc tcggccaggc tcccggtgag gacgatggcg gtcgagctgg ctagcggggt
     3601 cctgccacga gtacaagggg gaaccgtcaa gccgtcggac atgccgacca cgatggccgg
     3661 acggcctgac tacccgtgaa gtgcttctcg acatgcgatg tctgcagcat ggaatcgtcc
     3721 tagttgtgtt gatcctcctt ctttggtacg cttgatggac gcgacgcact ggtcggcggg
     3781 ctgtgagcga tgccggtctt gcgggccgct tgcttcaacc gccgcacgtg cgtgcgctgc
     3841 cctttggaca atcgtttggg tttggtcttg cttgtcttat ctgccatcga tgtcgactcc
     3901 ttattctgtc aatgatctag gaggccgctc tcgccccacg ttatacatcc cgatcacaat
     3961 atctctcacc gtattggaag cagtgaagtg tcgctcgata tgcggtgttt ggggcacggt
     4021 ggtttccttc aactgaacgg gggagtgccg gcgattagct tgtcattgcc tttcaggttt
     4081 gttctccttt gcttcgagtt gaaagtgctc gttccgcgtc atggcaggca tcaacctttg
     4141 agccgtgctg tccagaactc atccgcctcg gcttccagct tgtccaggcg agtgtaatag
     4201 tccgggaact ccttgaggtg tgcccaggca atcttgccgg tgagaggcag gtcgtcattc
     4261 gtgacgttgg tctccgggtc ccgcgcacca tgttccaact caacttcaag gccgcggcgg
     4321 aactgctcga gatcgatctt ctgccagtct aggccaagtg tggcgccaac ttgctgggct
     4381 tcttctgcgc taatccgacg tttgttgctc acgatgtacc tcgctctcaa taacatgttt
     4441 ctacccagtg cgcggcccgg catgaatccc tgtgcgtcgc ccaaccttcg gataagcttc
     4501 tccagtttac gctgccgcgc agcggcggtg ccagggggag agcgcggagt ggattttctg
     4561 aaaatctctg cgttctccgc gatcgctgcg gtgaaattgc gtaaggtgag ttactgaatc
     4621 agcccctccg gcactccagt ggggaacgcc tcctcgatcg ccgcctggat ccgctcgatg
     4681 cggttctcgg gattgggatg ggtgctgaag aattcaggcg gctgctgacc ctgactagct
     4741 tcggccaaga tctccatcac ctgcatcatc gagcggggat cgtagccgga ttcggccatg
     4801 aaacgcactc ccaaccggtc cgattccagc tcgtcgtcgc ggccgaactt caagttaatc
     4861 agctgtccga tcagcacagc cacctgcgcg gtctgctggc tggacggatc attcggatcg
     4921 tacgcggcga tcaccgccgc gccagtcaaa ccctcggtca gccgggcctt ggcgatatgc
     4981 tccgctgaat ggcgggccac cacgtgaccg acttcgtgcg ccagtacacc cgccagttga
     5041 ccttcggtct ccaggcgatc cagcagcccg cgcgtgatga agaccgggcc gccgggcaag
     5101 gcgaatgcat tgatcgtctg cgggtcggcc agtaagtaga attggaatgg gtagtccgta
     5161 tcagtagccg ccgtctgtgc gaccagctgc tggcccaccc gatcgaccag cgcctgagcc
     5221 tgttcatccg gatctaatcc tccgaattgc gccgctatct cgggagctgc ttgcaggcca
     5281 agcgcaatct cctgttccac ggtcatgtcg atatgctgta cttcgtcggt ggccgggttg
     5341 tagacttgcg cgccgaagta accgatcagc gagatgatgg cgatgaccgc cccgataacc
     5401 aggcggggca ggattgacga gcctcgccca cccgagttac gttgaccgaa catgagatct
     5461 cctcattgag gacggcttaa cgttcagctg cgccgttacg tttcgttctc gccgggtctg
     5521 cgatccagca ctcaggccag cggatcagag acccgctgcg aacaatgcat ctaccctgcc
     5581 atcagaaaag tcaggatggt agtgcctgtg tagggcacgg cagccggata atcaaaccga
     5641 aaacactaaa acacaacagt cgtgtctctt aaaatgtcaa atatcgtctg tgtcagtttc
     5701 atgttgctca cctccttagt gtgagaagtg cgattcgtaa taccgcaggc cgcggtgtcc
     5761 cacggcacga gtcgactagc ggtgcgaccg attccggccg cgggcgtccc tctgccgctt
     5821 gtgctgtgcc gcagctacaa cgctaaggtg tctgctggac agagtgaaag accgtcagcg
     5881 tccgcacagc cctccccggc ggacgggatt gacggccgcc gccagtagga ggatggcgcc
     5941 ccccaacgac accagcaaag ccggcgcgct gaagttggcc tggttgattg tgctgatccc
     6001 aaaatagagg ggtgaggaac caaccggcca cgaacgcgcc cacgacgccc acaaggattt
     6061 tgtaagacta ctcgctgagc gcctgtccca ctatcagtgg gaccctggtg cttactcggt
     6121 gcggtcatcc tggatcgtcg tttgagtgac gtccactggt tcacgccggc ggtagaaaat
     6181 cacccagacc agtcttgcaa tagcccatcc cgccaaggca tacaccacca ttgcgatgat
     6241 ggaatggagt tccagcaccc cactgccgac cgctggcgtc ggcgtcaacc ccgcaaaggg
     6301 gaacacgaaa atgttggtga gattgtagat gaaggcggca aatgggttat cgggggtggc
     6361 cgctatcagc ttcagcccga tgcgtagcgc gatgaggact tcaagcacgc cgacaagcag
     6421 ccagatcaat tggctggctc taaagttgcg tcgttctcgt tctgacattt tgaccttcct
     6481 tgctgagcat ggtccctatt gaaggctgag gactcagctc acttgtgaga tacacggggc
     6541 gtccgtgttt gcaatactgc acgcgctcag tgcccatcct cctatcagcg gaaacctgat
     6601 ggagctgtag gacggagact gatttatctg tcttcggttt agatcgttcc ttctgcctcc
     6661 cacacttgtt caggaaggcg agcgacgaaa cgtttcagcc gttggtgaca ctctcagccg
     6721 atgggggata aagggtcaag cgtctttcgc caatggcgat atacaacgct tgcaaggtgg
     6781 gatactgccc taccaagtat gttcccccgt ccatacctgg cagtatttct acgccgtacg
     6841 gtctgagaat tgcgaccgcg atggtccacg ccccagccgg ccagcccaac tgtgcggcgg
     6901 gcgggatgcg gttggattgt gcacccacgt ggttgatcgc atcgcgtaat agcttcatcg
     6961 ccaacttgca ccgcgctgct tcttcgcgtg cctccgcacg gtcagccagc ctcttgtcgc
     7021 cgttggacag aatgacgatg acaggcggcg cttcggtgtc gatcatcggt gtgtgactgg
     7081 cgagggagcc gctggtgtca tcgcctttgc cgttgccggg cgtgttcaac agttgcggcg
     7141 tggcgaatcc cgtttgggga tcgtcggcaa agacaattgt ctctgtgggc gcctcaaaca
     7201 gcgtgccaag cagggcaagt ctctggttct ccaagctgcg cctctcattg gccgcgtcga
     7261 tcattccgcc gatgaacttc gcccccaaca acaacaccaa cccgaacgcg atggccgcta
     7321 cgataggtat gagccatgac atcaactcgc tttgccgccg tgacgtgctg gcctcttggt
     7381 ccgccgtcac gccctcacgt tgcatggcct gagccgttgc cgtcgcctgg gcttgttctt
     7441 gcgccaggag catcgcccgc gcgtcgatgg ttgctttcgc ctctatcgcc tgcgcggtct
     7501 gttgggcgga catcgcctga gccgttgccg tcgcctgggc ttgttcttgc gccaggagca
     7561 tcgcccgcgc gtcgatggtt gccttcgcct ctatcgcttg cgcggtctgt tgggcgaaca
     7621 cggccgactg cgtggatggg aagttcgcgg cgtcggcggt catggcaagt tgcacgcgcg
     7681 ccacgcccaa cgcttgggcg gtcgccgtcg cggcaagtgc cagtacgccg tcagcggctt
     7741 gggcttgcgc cgtctcattg accgccgctg catgcgcatt ggcttcctgc tgttgcgcct
     7801 gttgcatggc gtaatcggcc ctggccttct gtgaggccgc gctgttatct aacgcggggt
     7861 cggacgtgac gaatctgcca ttcgctaagc acgcggcgat cgtcagcgcc atagctaaca
     7921 atatcaagga aagtttcatg gttgcccttt acgcctgacc attcatcggc atagcgtggg
     7981 tgtgcggatg agctatcgat tagcagcgcc ccatccgctt acaaccccgg ttacgcctgt
     8041 tatagccggc ccattagaaa caggaccagc acaaccacta gaatcagcca caaccttgga
     8101 aacattctcc tttatggcat cgtcggaata gcatctcggc tattctgaag ctgttagccg
     8161 ctggaaaggg tgcgtgtcgt gcgctcgttg cctgtgccgc cggggatctg ctcacgcacc
     8221 ggaagttgtt ctggttgatc ttgatcgtgc tgatcacgaa ggcgcatcga ccggttgttc
     8281 tgtactacgc tgcgggcggg cggcggcgga acaaccggtc tcg
//
LOCUS       DBNHXB010000294        22719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3431933, whole genome shotgun sequence.
ACCESSION   DBNHXB010000294 DBNHXB010000000
VERSION     DBNHXB010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 22719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22719
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3431933"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..2529)
                     /locus_tag="AC162G_11755"
     CDS             complement(142..2529)
                     /locus_tag="AC162G_11755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020266126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR5076539.1"
                     /translation="MGTAIGQRLPKLDAPAKATGRAVYGHDVRLPGMLHGRILYARYP
                     HARILSVDVSQAARLPGVKAIVTAADNPTTKFGYGKDNTPLKGDVARSLRDEIAAVAA
                     VDADTAEEALELIRVEYEPLPAVFDAGSALQEGAPLVHLQHGSNLFQTYRYAHGDPER
                     GERESDVIVEADYDLPYVAHAAMETSVVVASFDLGGRLTLYSTTQIPFLLQRDLSEAL
                     GMNGSDIRIIQTAIGGAFGRGLDIYPYEPIAALLARKAGKPVRVAFSRREEFLAAPMR
                     QPAKVHVRAGAKRDGLITFRDVDALLDIGAYVSWGSVTPLVMLETTASLYRVPHVRFK
                     ADCVYTNNPITGAVRGYGNPQSTFFVETTMDRLAEALGIDPVDFRIRNANRPDEETPQ
                     GLKITSCGLKECLEAVAARADVESMEVGSEKLEVGGRAPDKSRASNLQSPISNLKKGI
                     GFAATLNVGGGARIYRSDGCGATVKVDDFGHVSLITGSTEIGQGSETVLAQIVAEVLG
                     VKAEAVTVLNSDTDVKPWDVGVHASRTTFIAGNAAHVAAMHARAQIFETAAEMLGVSP
                     EQLVARDGMVTVAADGEPDSKRQTPKSKIQNPKAVELGKVVRARHFREGGQIVAGEGW
                     YDPPTQLVDKATYKGNISATYGFGAQMAEVEVDTETGHVRVLRLVCANDVGRAINPMA
                     VEGQMEGGAQMGLGYALCEEVQVQDGQVLNPNFHDYRLFTAADMPHIESIIIETDDPQ
                     GPFGAKGVGEMGGTPTAAAIANAIYDAVGIRMTTLPMTPERVLKGLMELREVREL"
     gene            complement(2535..3059)
                     /locus_tag="AC162G_11760"
     CDS             complement(2535..3059)
                     /locus_tag="AC162G_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018032235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5076540.1"
                     /translation="MPKLVLTLTVNGEPRELLIDPRRTLLDVLRNQLELRGTHRGCDT
                     GNCGACTVILNGRLVTSCMVLAADCDGAEVLTVEGISPDGQLHPVQRTLVEQGGIQCG
                     YCTPGMVMASVALLNANPHPTQEEVRMGLAGNLCRCTGYTKIVEAVLAVAGERDSWEL
                     RELTPSVSEDPSFR"
     gene            complement(3100..3948)
                     /locus_tag="AC162G_11765"
     CDS             complement(3100..3948)
                     /locus_tag="AC162G_11765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR5076541.1"
                     /translation="MKSFEYFAPRTLAEAIETLARYNGQARVLAGGTNLVLNMKEGND
                     SPRAVVNVKRIPELRGLEFNSHLTLGALTAAEEIRCSPLIRERFPALAHAASTMASVQ
                     IRNLATVGGNLCNAAPSADLAPILIAFDAVARISGPGGERRVPLEEFFTGPGATVLSA
                     ADLLMAVEVPPWDGPAWYLKHAPRAWMDIAVAGVACALRMEGGVCADARVVLGAVAPT
                     PLRVRRAESELVGGPLIPERIDRAAKIAAEESKPIDDVRGSAWYRRQMVEVLTRRGLM
                     QLITDR"
     gene            complement(4081..4623)
                     /locus_tag="AC162G_11770"
     CDS             complement(4081..4623)
                     /locus_tag="AC162G_11770"
                     /inference="COORDINATES: protein motif:HMM:NF013707.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR5076542.1"
                     /translation="MELKLIITGIGGQGVVYATKVLSHAALARGERVMVSENHGMSQR
                     GGSVMAHLKLGGHEAPLIRRGTADALIGFDRVETLRNLTFIRPGGSVFVNTANELEAA
                     ATDRLNELGVGVFTIDAGACAKELGAPAAANLIVLGFAAAHQRLGLSVEDLKAAVRAL
                     GPVKAIELNLKALEMGAARA"
     gene            complement(4704..6632)
                     /locus_tag="AC162G_11775"
     CDS             complement(4704..6632)
                     /locus_tag="AC162G_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5076543.1"
                     /translation="MSAQLLLGNDAIALGLIRNGCRMVTAYPGTPSSEILAGVVKFKK
                     QLDRSIYTEWSASEKVAFEVALAASWSGLRAATAMKQVGLNVAADSLFSAAYTGVVGG
                     FVVVSCDDPGPQSSQTEQDSRLLALTAKVPVLDPSTPAEALAMIRAAFELSERHRMPV
                     MLRPTTRVCHAVQSMEVNGSLPSPADGKLGFKKDPRRWAATPVFRLKLHHELNAKLEA
                     IEREFESSPLNYQSPITPLQDMGNWRLGHWGLGIIASGVAYATAQECLAELGVTVPIL
                     KIGTPFPLPRKRVMGFIEQFERVLILEEPDACLELQIPDRTRVSGRLDGTVPNAGELA
                     PEVMMSILAQALNRAGIAASPPSADAALSTLVQSFKLAPRRPRLCPGCSHRSAFFTIK
                     QTFGPNAIYPGDIGCYTLGTNLRVVDTCVDMGASVTMATGFYHASRLTRDKRPIVATI
                     GDSTFLHSGIVPLANAVHTNARFVLVILDNHTTAMTGAQPTPASDYTADGDIATTVPI
                     PALVEACGVKFVRVADPYDHGPFAALLKEAHAFTQAPEGGPAVIIADRPCILYDPSPV
                     QAEPVPVVVTEQCDGCRYCVEAFECPALVLRADRSRVDINTSVCVDCGQCIDACYKGF
                     IVPQAPVTSYQSSVISNQ"
     gene            complement(6703..8076)
                     /locus_tag="AC162G_11780"
     CDS             complement(6703..8076)
                     /locus_tag="AC162G_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HIR5076544.1"
                     /translation="MSDQHKFLLDESQVPKAWYNINPDLPQSPGPLLHPATKEPVTPD
                     FLAVLFPMGLIMQAVSMDRWVEIPDPVLDVYRLWRPTPMYRALRLEKMLDTPAHIYYK
                     YEGVSPVGSHKPNTAVAQAFYAKQEGVTGYTTETGAGQWGSALAMACNFFGLECQVYM
                     VRVSYHQKPYRRIIMETFGASVVPSPSDKTQYGRKLLAEYPDHPGSLGIAISEGVEAA
                     VTSNGKYKYSLGSVLPFVLMHQTVVGLEALQQMEMAGEYPDMVIGCIGGGSNFAGFVF
                     PFLHQNFTAGKKTRVIAVEPMACPSLTKGKYTFDYGDTAETAPIAKMHTLGHTFIPPG
                     IHAGGLRYHGMAPHVCALYDHGDIEAVAVHQVPTFEAAVQFARAEGILAAPEAAHAVR
                     VAIDEALKCKRDGTKKVIAFNLSGHGHFDLGAYDAYHQGKLEDYEYPAELVEEAMKHL
                     PEVSIPA"
     gene            complement(8263..8586)
                     /locus_tag="AC162G_11785"
     CDS             complement(8263..8586)
                     /locus_tag="AC162G_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076545.1"
                     /translation="MTDADYRTELSDEACPEPRRRDECVIEEPVPRWPDFERPPDPRL
                     VAEGWERRFMADSQRLNEFKDLYISMGYEVRAEPVRPEEIGPECGDCQLVLCRLYFTL
                     YTRKL"
     gene            complement(8620..9450)
                     /locus_tag="AC162G_11790"
     CDS             complement(8620..9450)
                     /locus_tag="AC162G_11790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HIR5076546.1"
                     /translation="MIYTAGIDVGSTYTKAVVLSEENQIAGRGLLNTGFKLAEAGSRA
                     LARALDEAGLTQDDLAYVASTGYGRFQVPFRNVNVTDLTAAAHGTRFFFPGTQTVLDV
                     GGQTMKASRLDGAGKVKSFRLNDKCAAGTGAFLEKTARYMGFTTEEIGPLVATSKEVV
                     PISGVCAVFAESEVINCLSQGYAPADIMHGAIVSLVDRSVQLMKRVQVEPEVTLIGGI
                     LRFETMAGVVREKLKMAVNVPEGDLVQFTSALGAALLARQRLYKVGEQDVPAESALAA
                     "
     gene            complement(9447..10271)
                     /locus_tag="AC162G_11795"
     CDS             complement(9447..10271)
                     /locus_tag="AC162G_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HIR5076547.1"
                     /translation="MAYAAGVDVGSTQTKAIILDENRQIVSRSLIDTGANVVLAAENA
                     YAEALRNGDIREEEVQYVVGTGYGRYKVIFGDTQITEISCHGRGSVHMFPGTRTVVDM
                     GGQDTKAIRVSAKGEILDFCMNDKCAAGTGRFLGAAAAALEIPLTELGPTALRADKPV
                     KISTTCTVFAESEVISWLGKGKKIEDILLGVHQSIVSRSMGLMRRVGIESEVTFTGGV
                     ALNVGVVQVLNDALGFPVNVGAESHFMGALGAALFAMDRILSRRVPAGAVRAEEAA"
     gene            complement(10377..11672)
                     /locus_tag="AC162G_11800"
     CDS             complement(10377..11672)
                     /locus_tag="AC162G_11800"
                     /inference="COORDINATES: protein motif:HMM:NF017828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HIR5076548.1"
                     /translation="MTDRQSTDTIVGRGAAEGARLFREWFKSLTEAPEHGKKAAYVFV
                     MGSLAEVLRVFDFHTVFPEINSLQTAVRRVAHEYLNTAEDYGYSPDICGYVKADVGMQ
                     LRGGEHPMGRIPPPSLAVYTNACNTYIKWAEIWERMYHIPIFTLDVPGSRAAGGQTWP
                     GDPDFENDKRYVAAQLKELIALCEEVSGKHFDIDRLREVMREANVMSRAWKRVLELNQ
                     SVPSVFNAVTDGTVYLGMANGFRGTPEGAKYFEEVVEEMEYKAANGLGTLHEEKYRLI
                     FVGVPCYPIFRRFNEMFAEQGGAFVSSTYLWFASGGTSLGYEYDLSRPLDSLAEGLLI
                     SVRHAMDSMFFQDRTLIDMVDPYRIDGVVYHPIKSCRTVSTGLADNRRSLLAARDVGS
                     LFIESDMMDKRVVSEAQLKNRIDAFFEGLATRKHMADSR"
     gene            complement(11728..12894)
                     /locus_tag="AC162G_11805"
     CDS             complement(11728..12894)
                     /locus_tag="AC162G_11805"
                     /inference="COORDINATES: protein motif:HMM:NF017828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HIR5076549.1"
                     /translation="MNVRTLDSRPYEPLDAVLDMCRELVEDPEYPTVRRWREAGGKVL
                     GHFQVYFPEEIAHAAGMLPLKIRGAQIEARQAEARFGSYLCSILKTSLELALSKRIEI
                     DMFVTHPICDAARNLAAVWGRNFPYVCQILYLPQNANSSHAAAYLRDEYARLKREIEG
                     VAGRKVGDDDLRASIRVYNENRRLLRKLYAIKREKPWLLPIDEAYVLVAVGGMILREE
                     HNDMLKAVIPQIEARTAKPLDRIRLVFEGGFCEQPPLDLLRAIAQSCYVVDDDLFIGL
                     RWILEDVPLEGDPLFNLAEAYLERSSYSPVQHDLRKPKEHMLLQRIKEANASAAVITA
                     AKMCEPGLDEQVAYVKALDAKGIPYFVSEFEESMTSFDHLQIQLETFVENLLFA"
     gene            complement(12990..14033)
                     /locus_tag="AC162G_11810"
     CDS             complement(12990..14033)
                     /locus_tag="AC162G_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR5076550.1"
                     /translation="MSFIQTRTVEFTEILYAKSDWIARITINRPQAYNAYSTRALMEL
                     AAAFREAAFDDEVAVIVYTGAGDRAFCTGGDVKEYEEIYTRSPRDYWKYMGLFSAYIE
                     SIINTGKPVIARVNGMAVGGGNESHLACDLSVIAEHAYIGQVGTGVGSVAAGGATQWL
                     PIFVGDRRARWMLMLNRRIPPYQALEWGLVNEVTPSVKKDGEFVVRATPDQIAKAQKG
                     QDGYCLDLSRLDQAVDALCRELVDKFPECTRYTKQQANFWKDLAWHQTVGHARDWLSI
                     HFATWEPLEGMGAFVEKRPARYRMLRERAAAGQSSEFPWGPYARECPSCGTKYLPDEF
                     DFCGKCGARVSNQ"
     gene            complement(14113..14958)
                     /locus_tag="AC162G_11815"
     CDS             complement(14113..14958)
                     /locus_tag="AC162G_11815"
                     /inference="COORDINATES: protein motif:HMM:NF019829.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator"
                     /protein_id="HIR5076551.1"
                     /translation="MSKRRQVRTQFLIFTLFGDYVLPREGWAWTRSLIELLGLLGVSS
                     QAARSTLSRMSKQGWLKPQRRGRNSLYALTPKGRRLLEEGAQRIFEPRRQEWDGQWRV
                     VVYSLPERKRDLRETIRRRLSWLGFGPLAPGMWVSPHDRHTELGALLDDLHAWRYVQC
                     FVGRWLSDLSCGDLVARCWNLGDLNQRYAAFLRKWEPELQSCEERLSTGNALRPDDCF
                     VRRFWIIHEYSTFPRSDPNLPAELLPEGWLGDRATLVFREYHRLLSGRANEFIEKTLG
                     NGSKP"
     gene            complement(15046..15846)
                     /locus_tag="AC162G_11820"
     CDS             complement(15046..15846)
                     /locus_tag="AC162G_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR5076552.1"
                     /translation="MFRSLRIQLGAIVFCFVLLLAGSVAITYLIVQTQASGATLRALL
                     LIQLVLLVAALLLLTWGYFFTRRRFARPLATLNRAAHRIAEGHLVEPVRVEGGDELAD
                     LARAFETMRADIAASRDELESRVAQRTRELASAFEFSQEIVSQLDLRHLLQSVTDHAR
                     TLTRAEAASLCLLDEGNTALVLSSTSGAKVPPPDMRQPVWLVPADQGTSSGRMVAVER
                     VFIVGDSLFAETLSPLLASSRSIQVVGSAPTSEAAARLRGYCEKKNFL"
     gene            complement(16090..17931)
                     /gene="glmS"
                     /locus_tag="AC162G_11825"
     CDS             complement(16090..17931)
                     /gene="glmS"
                     /locus_tag="AC162G_11825"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR5076553.1"
                     /translation="MCGIVGYVGSRDATPIIFNGLKRLEYRGYDSAGIAVIENGHIAV
                     RRDAGKLDRLASLLEAEPMHGPIGVGHTRWATHGQANQTNSHPHVDCTGRLVVVHNGI
                     VENFLALRHELQAAGHVFRSETDTEVIVHLVEQYLRDGETFEEAARKALNRLQGAQAV
                     VLLDHDQPDRLIVSRIGNAGGVTVGVGQGEMFLASDMPAILEYTRDMIFLESRQMAVV
                     LRDGVKFVSLDGEPLELPIHTVPYDPVAAAKGEYTHFMQKEIFEQARSITDTIRGRVD
                     FERGVVSLPEMNLSSEAARSIDKIISVACGTSWHAALVGKYMIESLARVPVEVDYGSE
                     FRYRDPILDDRTAVLAITQSGETVDTLAAMEQARMKGARLWSIVNVVGSQAHRISDGS
                     ILIQAGPEIGVASTKTFTGSQVDQYVLGVYLGQLRGALSGERLRAAVDALAKLPDLAG
                     RLMDRAPEVEALAAHFYHYTDFLFLGRGINFPIALEGALKLKEISYIHAEGYPAGEMK
                     HGPIALIDEDMPVVCIALRDHVYDKMISQVEQVKARGGTVIALATDGDEDIRRKADHV
                     LYVPECAPLLAPVLASIPLQMLAYTIAVRRGCDVDQPRNLAKSVTVE"
     gene            complement(18500..19267)
                     /locus_tag="AC162G_11830"
     CDS             complement(18500..19267)
                     /locus_tag="AC162G_11830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5076554.1"
                     /translation="MTSTTDVKRRGRFRSKRAGRASKYQNYKPSLLRRFYTLFLRVAF
                     KVTMRLTIEGLEHFPASGACIAMFNHIAFVDPVIVSGRFPRSVIALAKIEAYQHWLTG
                     PLLRTFDAIPVSRGDVDRKALQAAFDVLDQGLPLLIAPEGTRSKTATLLEAQPGLAYI
                     ASQAGAPIVPVAFSGSDRWSRNIKRLRRTTIRVRVGRPFVLAADEERLSGETLKQMTD
                     EAMYQLASLLPAEQRGVYSNLDAATARYVRFLQAAPD"
     gene            complement(19264..19959)
                     /gene="cmk"
                     /locus_tag="AC162G_11835"
     CDS             complement(19264..19959)
                     /gene="cmk"
                     /locus_tag="AC162G_11835"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR5076555.1"
                     /translation="MKPSVIAIDGPAGSGKSTLGHLLAQRLGYLYFDTGVLYRAVSLA
                     ALEHGVSVDDDVSLTELARRVQIDVVEPTAADGRLDTVLLDGRDVTWDIRSPAVDAIV
                     SPVSAVAGVRRALVDRQRRIAQRGKVVMVGRDIGTVVLPDADLKLFVDASLEVRAQRR
                     HAELLRRGEQATYEDVLDEMRRRDVRDREKPISPLVAAPDAITLDTTKLDMSAMVDRA
                     EEIVRRFAEAKVT"
     gene            complement(20117..20914)
                     /locus_tag="AC162G_11840"
     CDS             complement(20117..20914)
                     /locus_tag="AC162G_11840"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR5076556.1"
                     /translation="MPSERLQKLLAHAGYGSRRACEEIVAAGRVTVNGKVMKLGDKAD
                     PAADTIAVDGQRLKIESPPVYIILNKPRNVVTTNEDELGRTTVRDLVDYDGQIYPVGR
                     LDADSEGLVLLTNDGELANVLTHPRYEHEKEYLVFVKGRPDAKALDVWRRGVVVPDPE
                     GAYKTAPARVEVVRHEGGGTLLRVVMREGRKRQIREVARLLGHPVQYLLRVRIGPIDM
                     GDLKPGKWRFLNAKEIRSLRGLVEKSQSGPTVVKRKSRFGRAVKREM"
     gene            complement(21038..21280)
                     /locus_tag="AC162G_11845"
     CDS             complement(21038..21280)
                     /locus_tag="AC162G_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076557.1"
                     /translation="MALSFFLSYAIAGLVGGASTGLVLRWVEPAIRWSQVIGIAVGWA
                     VAVVLGGFFPSGVISGAVAGALGAGVLYAVLRRART"
     gene            complement(21318..>22719)
                     /locus_tag="AC162G_11850"
     CDS             complement(21318..>22719)
                     /locus_tag="AC162G_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0015630 - microtubule cytoskeleton
                     [Evidence IEA];
                     GO_process: GO:0000226 - microtubule cytoskeleton
                     organization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MAP7 domain-containing protein"
                     /protein_id="HIR5076558.1"
                     /translation="DPRPWVREGAVRELDRLLHGSQLGLAQAARDALTRLADDDSRRV
                     STAAAGILATYADEQHAREAAQAGSARIARDKAEADRLAAKKAEEERLAREARRVEQA
                     RMAAQRAEADRVAREKVERERLAQQTAEAERLAAQKTQAERIAPEMQTGRTVEIDSGA
                     GLTNAIGIPVLLTTIGLGLSWAIAARTLSSEDVIASFVFGVPVPLTVIIVLALRHQGV
                     IVKWNQALVALVGWIIGVVAGGFAVALLISLIVLSGLTTQEANLTSGAVFGAGSGTIG
                     GLITGWVLKRIKPLIQIRVITVGWGLAWTLGWSIWSIVLVTMTDASAEEAVSAMAVTG
                     TIVGAIGGGVMFWHLNQVSGLKSESGLRVAPAVTQTADIFMRRRSTGAEVGLRVQAAG
                     VEPRPQPEWPMLLLIPVGWIIAWAIFPPSVLRGAIGGFITGASLRWTTKALQEKHVFL
                     IAAGWAIAWWIWLIFL"
BASE COUNT         4145 a         7558 c         6735 g         4281 t
ORIGIN      
        1 cgtcgagctc gagctcttcc tgcacctctt cgatcgactt gccggcgatg tacagcggaa
       61 ccttcagtag gttaggattt agatgaagct gtgacacgat gctctccgtg acaacggcaa
      121 gaactgaagg aactcgggaa ctcaaagttc cctcacttcc ctgagttcca tcagtccctt
      181 gagaacgcgc tccggcgtca tcggcagcgt ggtcattcgg atgcccaccg catcgtagat
      241 cgcattggca atcgctgcgg cggtcggcgt gccgcccatc tcgccgacgc ctttcgcgcc
      301 gaacgggcct tgcggatcgt ccgtttcgat gataatgctc tcgatgtgcg gcatatcggc
      361 ggcagtgaac aatcggtaat cgtggaagtt aggattgagc acctggccgt cctgcacctg
      421 tacctcttcg cacaacgcgt agcccagccc catctgggcg ccgccttcca tctgcccttc
      481 gaccgccatc gggttgatcg cccgacccac gtcgttcgcg cataccagcc gcagcacgcg
      541 cacgtgcccc gtctcggtgt ccacttccac ctcggccatc tgcgcgccga agccgtatgt
      601 cgccgaaatg ttgcctttgt aagtggcctt gtcgacgagc tgggtcggcg ggtcgtacca
      661 cccctcgccg gcgacgatct gcccgccctc gcgaaagtgc cgcgcgcgga ccaccttgcc
      721 caactcgacc gccttcggat tctggatttt ggactttggc gtttggcgtt tggagtctgg
      781 ttctccatct gctgcaacag tgaccatacc atcacgcgct accaactgct cgggcgagac
      841 gcccagcatt tccgccgccg tctcgaatat ctgcgctcgc gcgtgcatcg ccgcaacgtg
      901 cgccgcgttg cccgcgatga acgtcgtccg gctggcgtgc acgcccacgt cccacggctt
      961 cacgtcggtg tcgctgttga gaacggtcac ggcttcagcc ttcacgccca acacctcggc
     1021 gacgatctgc gccagcacgg tctccgatcc ctgcccgatc tcggtgctgc ctgtgatcag
     1081 ggatacgtgc ccgaagtcgt ccaccttcac cgtcgcgccg cagccgtcgc tgcgatagat
     1141 gcgcgccccg ccgccgacgt tcagtgtggc tgcgaaaccg atccccttct tgagattgga
     1201 gattggagat tggaggttgg aggcgcgact cttgtccggg gcacgccctc caacttccaa
     1261 cttctcactt ccaacttcca tgctctccac atctgctctt gcggcgaccg cttccaggca
     1321 ctctttcaag ccgcacgacg tgatcttcag accctgcggc gtctcttcat ccgggcggtt
     1381 cgcgttgcgg atgcggaagt cgacgggatc gatacccagc gcttcggcca ggcgatccat
     1441 cgtcgtctcg acgaagaagg tcgattgcgg attgccgtag ccgcgcaccg cgccggtgat
     1501 cgggttgttg gtgtacacgc agtcggcctt gaatcgaacg tgcggcacac ggtacagcga
     1561 ggctgttgtc tctagcatca ccagcggcgt gacgctgccc cacgagacat aggcgcctat
     1621 gtcgagcagc gcatcgacgt cgcggaaggt gatcaaccca tcgcgtttcg cgcccgcgcg
     1681 cacgtgtacc tttgccggct ggcgcatcgg cgcggcgaga aattcctcac ggcgcgagaa
     1741 cgccacgcgc accggcttgc ccgccttgcg cgccagcagc gcggcgatcg gctcgtacgg
     1801 gtaaatgtcc aacccgcgcc cgaacgcgcc gccgatcgcc gtctggatga tgcgaatgtc
     1861 gctgccattc atgccgagcg cttcagagag atcccgctga agcaggaatg gaatctgcgt
     1921 ggtgctgtac agcgtgagcc ggccgccgag gtcgaacgag gcgacgacga cgctcgtctc
     1981 catcgccgcg tgcgctacat acggcaagtc gtagtccgct tccacgatga catcgctctc
     2041 gcgctcgccc cgctccggat cgccgtgcgc atagcggtaa gtctgaaaca gattgctgcc
     2101 gtgctgcaga tgaacgagcg gcgcgccttc ctgcaacgcc gatccggcgt cgaacaccgc
     2161 aggcagcggc tcgtactcga cgcggatcaa ttccagggcc tcttcggcgg tatcggcgtc
     2221 taccgcggcg actgccgcga tctcgtcgcg caggctgcgc gccacgtccc ccttgagcgg
     2281 cgtgttgtcc ttgccgtagc cgaacttcgt cgtcgggtta tcggccgccg tgacgatcgc
     2341 cttcacgccc ggcagtctcg ccgcttgtga aacatccacg ctcaaaatcc gcgcgtgagg
     2401 atacctcgca tacaggatgc gcccgtgcag catgcccggc aggcgcacgt cgtgcccgta
     2461 gaccgcgcgt ccggtcgcct tagccggcgc atcaagcttt gggaggcgtt gaccgatagc
     2521 tgtacccata cgttttaccg gaatgaggga tcctcagaaa cagagggggt gagttccctc
     2581 agctcccatg agtccctttc tcccgccaca gccaacaccg cttcgacaat cttcgtgtaa
     2641 cccgtgcagc ggcacagatt gccggccagt cccatccgca cttcctcttg ggtgggatgc
     2701 ggattcgcgt tcaacagcgc gacagaggcc atcaccattc ccggcgtgca gtaaccgcat
     2761 tggatgccgc cctgttccac caacgtgcgc tgcacgggat ggagttggcc gtcgggcgat
     2821 atgccctcga cggtgagcac ttcggcgccg tcgcaatcgg cggcgagcac catgcacgac
     2881 gtgacgagcc tgccgttgag gatcaccgta cacgcgccgc agttaccggt gtcgcagccc
     2941 cgatgcgtgc cgcgcagctc gagctgattg cgcaggacgt ccagcagtgt ccggcgcggg
     3001 tcaatgagca gctcgcgcgg ctccccgttg acagtcagcg tgagaacaag tttcggcatg
     3061 caaatgacaa cttactggtt actgataact gattactgtt caccgatcag ttatcaattg
     3121 catcaatcct cgccgcgtca gcacttcgac catctgccgg cgataccacg ccgatccgcg
     3181 cacgtcgtcg atcggcttcg actcttcggc ggcgatcttc gccgcgcgat cgatccgttc
     3241 ggggataagc ggtccgccca ccagttcgct ctctgcccgg cgaacacgca gcggcgtcgg
     3301 cgccacggcc cccaacacaa cccgcgcatc ggcgcacacg ccaccctcca tccgtagtgc
     3361 gcacgcaacg cctgcgacgg caatatccat ccacgcgcgc ggcgcgtgct tgaggtacca
     3421 cgccgggccg tcccaaggcg ggacttcgac cgccatcagc aagtccgccg cagagagaac
     3481 ggtcgcgccc ggccccgtaa agaactcctc gagcggaacg cgccgctcgc cgcccggacc
     3541 ggagatgcgc gcgaccgcgt cgaacgcgat aaggatcggt gcgagatcgg ccgacggcgc
     3601 cgcgttgcac aggttgccgc cgacggtggc gagattgcga atctgaaccg aggccatcgt
     3661 cgacgcggcg tgggccaacg ccgggaagcg ctcgcgaatg agaggcgagc atcggatctc
     3721 ttcggccgct gtcagagcgc cgagcgtgag atgagaattg aactccaggc cgcgcaattc
     3781 cgggatgcgc ttgacgttga cgacggccct cggcgaatcg ttgccctcct tcatgttgag
     3841 gacgagattc gtgccgcctg cgaggacacg cgcctggccg ttgtatcgag ccagcgtctc
     3901 gatcgcctct gccagcgtcc tgggagcgaa atactcaaat gacttcatga tagctgatag
     3961 cctatggcgt atcgcatatc gcgtatccct tcacgcctca cgctttacgt ttcacgcatc
     4021 acgcttcacg cctcacgctt tacgcttcac gttttacgtt tcacgcttac acttcacgcc
     4081 tcacgctctc gccgcaccca tctccaacgc cttcaaattc aactcgatcg ccttcaccgg
     4141 gccgagcgcg cgcacggccg cctttagatc ctctacgctt aaacccaagc gctggtgcgc
     4201 cgcagcgaag cccagcacga tgaggtttgc cgccgctggc gcgccgagct ccttcgcgca
     4261 ggcgcccgcg tcgatggtga acacgccgac gcccagctcg ttcaggcgat ccgtcgccgc
     4321 cgcctcaagt tcattcgcag tgttgacgaa cacgcttccg cccggccgga tgaacgtgag
     4381 gtttcgcagg gtttcgacgc ggtcaaaccc gatcaacgcg tcggcggtcc cacgccggat
     4441 gagcggcgct tcgtgccccc cgagcttgag gtgcgccatc acgctgccgc cgcgctgact
     4501 catgccgtga ttctccgaga ccatcacgcg ctcgccgcgc gccagcgccg cgtgcgacag
     4561 caccttcgtc gcgtaaacga ccccctgccc gccaatgcct gtgatgatga gcttgagttc
     4621 catatcagtg atcagtgatc agttatcagt catcagttat cagtcggcag ccatcaatca
     4681 ccagtgatca gctcaaccac tgatcactga ttactgatga ctgatgactg ataactggtt
     4741 actggcgcct gcggcacaat gaatccctta taacacgcat cgatgcactg gccacagtcc
     4801 acgcacacac tggtgttgat atccacccgc gaccgatcgg cgcgcaggac gagcgcgggg
     4861 cattcgaatg cttcgacgca gtagcggcag ccgtcgcatt gctcggtgac gacgacgggt
     4921 accggctctg cctggaccgg ggacgggtcg tacaatatgc agggccggtc tgcgatgatg
     4981 acggccgggc cgccttcggg cgcctgggtg aaggcgtggg cctccttgag cagcgccgcg
     5041 aacggcccgt gatcgtaggg atcggcaacc cggacgaact tcacgccaca cgcctcgacc
     5101 agagcgggga tcggaaccgt ggtcgcgatg tcgccgtcgg ccgtgtagtc gctggcgggc
     5161 gtgggctgcg cgccggtcat cgccgtcgtg tggttgtcga gaatcaccag gacgaaacgt
     5221 gcattcgtat gcacggcatt cgccagcggc acaatacccg aatgcaggaa cgtcgagtcg
     5281 ccgatcgtgg cgacgatcgg tcgcttatca cgggtcagcc gcgaggcgtg ataaaacccg
     5341 gtcgccatcg tcaccgatgc gcccatatcc acacaggtgt ccacgacgcg caggttcgtc
     5401 cccagcgtat agcagccgat gtcgccggga tagatagcat tcggaccgaa ggtctgcttg
     5461 atcgtgaaga aggccgatcg gtgcgagcag ccggggcaca gtcgcggacg gcgcggcgcg
     5521 agcttgaacg actggacgag cgtggacaaa gccgcgtcgg cagagggcgg cgacgcggcg
     5581 ataccggccc ggttgagcgc ctgcgccagg atcgacatca tgacctcggg cgccagctcg
     5641 cccgcgttgg gcaccgtccc atccaaccga ccgctcacgc gcgtccgatc cggaatctgc
     5701 agctcgaggc aggcgtccgg ctcttcaaga ataagcacgc gctcaaactg ctcgataaat
     5761 cccatcaccc gtttgcgcgg cagcggaaac ggcgtaccga ttttgaggat gggcactgtg
     5821 acgccgagtt cggcaagaca ctcctgcgcc gtagcatatg cgacaccgct ggcgataatc
     5881 cccaatcccc aatgaccaag tctccaatta cccatatcct gaagaggggt aattggagat
     5941 tggtaattga ggggcgacga ctcgaactcg cgctcgatgg cttccagttt ggcgttaagc
     6001 tcgtgatgca gcttgagccg gaagaccggc gtcgcggccc agcgtcgagg atctttcttg
     6061 aagccgagct tgccgtctgc cggcgacggc aggctgccat tgacttccat actctgcacg
     6121 gcatggcaca cgcgggtcgt cgggcgaagc atgacaggca tgcggtggcg ttccgaaagc
     6181 tcgaaggctg cccgtatcat cgccaacgct tcggccggcg tagacgggtc gagtaccggc
     6241 actttggccg tcagcgccag caggcgcgag tcttgctcgg tctgcgacga ttggggaccc
     6301 ggatcgtcgc aggatacgac cacgaacccg ccgacgacgc cggtgtacgc cgcgctaaac
     6361 aatgaatcgg cggcgacatt gagaccgacc tgcttcatcg ccgtcgccgc tctaagcccg
     6421 ctccagctcg ccgcaagcgc aacttcaaag gcgaccttct cgctggcgct ccactcggtg
     6481 tagatcgaac ggtccaactg cttcttgaac ttgacgacgc cggcgaggat ctccgagctg
     6541 ggcgtgccgg ggtaggccgt caccatccgg caaccgttcc ggatcaggcc aagagcgatt
     6601 gcgtcgttgc cgagcagaag ttgagcgctc atcgtcactc tgtcccccgc cccccgaccc
     6661 ttcccccgct gccacggggg aggggccggg gggtgagggc cactaagccg ggatcgaaac
     6721 ctccggcaga tgcttcatcg cctcctccac cagttcagcc ggatactcgt aatcttccag
     6781 cttgccttga tggtaggcgt cgtatgcgcc caggtcgaag tggccgtgac cagacagatt
     6841 gaaggcgatc actttctttg tgccgtcgcg cttgcacttg agcgcctcgt cgatcgccac
     6901 gcgcaccgcg tgcgccgcct ccggcgccgc caagatgcct tccgcccgcg cgaactgcac
     6961 cgccgcctcg aacgtcggca cttggtgcac cgccactgcc tcgatgtcgc cgtgatcgta
     7021 cagtgcgcac acgtgtggcg ccatgccgtg atagcgcagc ccgccggcgt ggatgcccgg
     7081 cggtatgaac gtgtgcccca gcgtgtgcat cttggcgatc ggcgccgtct ctgccgtgtc
     7141 gccgtagtcg aaggtgtatt tgcccttcgt cagcgacggg caggccattg gctcgacggc
     7201 gatcacccgt gtcttctttc ccgccgtgaa gttctgatgc aggaagggga atacgaaccc
     7261 cgcgaagttg ctccctccgc cgatgcagcc gatcaccatg tccgggtact ctccagccat
     7321 ctccatctgc tgcagtgctt ccaggcccac gacagtctga tgcatcagca cgaacggtag
     7381 caccgatccc aacgagtact tgtacttccc attcgatgtg acggcggctt caaccccctc
     7441 ggaaatagca atccctagcg agccggggtg gtccggatat tccgccagca gcttccggcc
     7501 gtactgggtc ttgtctgaag ggctgggcac gaccgacgcg ccgaaggtct ccatgatgat
     7561 tcgccggtag ggcttctgat gatatgaaac cctgaccatg tagacctggc attccaggcc
     7621 gaagaaattg caggccatcg ccagggccga gccccactgg ccggcgcccg tctccgtggt
     7681 gtagcccgtg acaccttctt gtttggcgta gaacgcctgc gccacggccg tgttgggctt
     7741 gtgcgacccg accgggctga caccttcgta cttgtagtag atgtgagccg gggtgtcgag
     7801 catcttctcc agtcgtagcg cgcggtacat gggcgtcggc cgccagagcc tgtagacgtc
     7861 cagcaccgga tcggggatct caacccagcg atccatgctc acggcttgca taatgagccc
     7921 catcggaaag agcacggcca ggaagtcagg ggtgaccggt tctttggtgg ccgggtgcag
     7981 caacgggccg ggcgactggg gcagatctgg attgatgttg taccacgcct tcggcacctg
     8041 gctttcgtcc aacaggaatt tgtgttgatc gctcatgatt ctctcctcca cgggttgttg
     8101 gcatacacac gagacaactc aagcaggctg gcgaccccgc tgggcggggc gcaagcagcg
     8161 atggttcccc aatagattcc ccctttccag tagtcagtga tcagtgatca gtgatcagtg
     8221 atcagtaatc ttgcagtgag tgaacctgac cgtgagcact ggctacaact tgcgcgtata
     8281 cagcgtgaag taaagccggc ataataccaa ctggcagtcg ccgcattccg gaccgatctc
     8341 ctccgggcga accggctcgg cccgtacttc gtagcccatc gagatgtaca agtccttgaa
     8401 ttcgttcagc cgctgcgagt cggccatgaa acggcgctcc catccttcgg cgaccaacct
     8461 cggatcgggc ggccgctcga agtccggcca gcgcggcaca ggctcttcga tgacgcattc
     8521 gtcccttcgc ctcggctcag ggcaggcttc gtcgctcagc tccgttcggt agtcggcgtc
     8581 tgtcatgtcg cggcggctcc ttgcccggat gcggccgtgt caggcggcca gggccgattc
     8641 ggccggcaca tcttgctcgc cgactttgta gagccgctgg cgagccagga gcgctgcgcc
     8701 cagcgccgac gtgaactgaa ccaggtcgcc ttcgggcacg ttgacggcca tcttcagctt
     8761 ctcgcgcacc acgcccgcca tcgtctcgaa gcgcaggatg ccgccgatca gcgtgacctc
     8821 cggctccacc tgtacacgct tcatcagttg cacggagcgg tcgacaagcg agacgatcgc
     8881 cccgtgcatg atgtccgccg gcgcgtaacc ttgcgagagg caattgatga cctccgactc
     8941 ggcgaagacc gcgcacacgc ccgagatggg cacgacttct ttcgacgtcg ctacgagcgg
     9001 accgatctcc tcggtcgtga atcccatgta gcgcgccgtc ttctccagga aagcgccggt
     9061 cccggccgcg cacttgtcgt tgaggcggaa cgatttcacc tttcccgctc cgtcgagccg
     9121 gctggctttc atcgtctgcc cgcccacgtc caggaccgtc tgggtgccgg gaaagaagaa
     9181 gcgcgtgccg tgcgcggcgg cggtcaggtc ggtgacgttg acgttgcgga agggaacttg
     9241 aaaacggcca tagccggtgc tggcgacgta agccaggtca tcctgcgtca gcccggcttc
     9301 gtcgagcgcc ctcgccaggg cgcggctgcc ggcctcggcc agcttgaagc cggtattcag
     9361 caggccgcgc ccggcgatct gattttcttc gcttagaacg acagccttcg tgtacgtgga
     9421 gccgacgtcg atgcccgctg tgtaaatcat gctgcctcct ccgcgcgaac ggctccagcc
     9481 gggacgcgcc ggctcaagat gcgatccatc gcgaacaacg ccgcgcccag cgcgcccatg
     9541 aaatgcgatt ctgcgccgac gttgaccggg aagcccaacg cgtcgttgag cacttgcacg
     9601 acgccgacgt tcagcgccac gccgccggtg aacgtcactt cagactcgat gccgacgcgg
     9661 cgcatcagcc ccatcgagcg cgacacgatc gattgatgca cgccgagcag gatgtcctcg
     9721 atcttcttgc ctttgcccag ccacgagatc acctcggatt cggcgaagac cgtgcaggtg
     9781 gtgctgatct tcaccggctt gtcggcgcgc agcgccgtcg ggcctagctc agtcagagga
     9841 atctcgagcg ccgccgctgc cgcgcccagg aagcgtcccg tgcccgccgc gcacttgtcg
     9901 ttcatgcaga agtcgagaat ctcgcccttc gcgctaacgc gaatcgcctt ggtgtcctgg
     9961 ccgcccatgt cgacgacggt gcgcgtgccg ggaaacatgt gcacggagcc gcgtccgtgg
    10021 caggagattt cggtgatctg cgtatcgccg aagatcactt tgtagcgtcc gtagcccgtg
    10081 ccgacgacgt attgcacttc ctcctcgcgg atgtcgccgt ttcgcagcgc ctcggcgtag
    10141 gcgttctcgg cagccagcac aacgttcgcc cccgtatcga tcagcgagcg actgacaatc
    10201 tgacgattct cgtcgagaat gatcgccttg gtctgagtcg acccgacatc tacacccgca
    10261 gcgtatgcca ttatatcctc ccttcccgcc tatcgcgtat cgctcatggc atatcgcgta
    10321 tcgcttatgg cttattgcta tatgctgcca ataggcgata cgcgataagc gatatgctat
    10381 ctgctatccg ccatgtgctt ccgcgtcgcc agtccttcga agaacgcgtc gatgcgattc
    10441 ttgagctgtg cttccgatac gacgcgcttg tccatcatgt ccgactcgat gaacaggctg
    10501 cccacgtccc gcgcggcaag gagcgagcgc cgattgtcgg ccagcccggt cgagaccgtg
    10561 cggcaagact taatcggatg atagaccacg ccgtcgatgc ggtacggatc gaccatgtcg
    10621 atcagcgttc ggtcctggaa gaacatgctg tccatcgcgt gccgcacgct gatgagcagc
    10681 ccttcggcca ggctgtccag cgggcggctc agatcgtact cgtaacctag gctggtgccg
    10741 cccgaagcga accacaggta agtggagctg acgaaggcac caccctgctc ggcgaacatc
    10801 tcgttgaagc ggcggaagat gggatagcat ggcacgccga cgaagatgag gcgatacttc
    10861 tcctcgtgca gcgttccgag gccgttcgcc gctttgtact ccatctcttc gacgacttcc
    10921 tcgaagtact tggcgccctc cggcgtgccg cgaaagccgt tcgccatgcc cagatacacg
    10981 gtcccgtccg tgacggcgtt gaagacagat ggcacactct ggttcaactc cagcacacgc
    11041 ttccaggcgc ggctcatgac gtttgcctcg cgcatcacct cgcgcagccg gtcgatgtca
    11101 aaatgcttgc ccgacacctc ttcgcacagc gcgatcagct ccttgagctg cgccgccacg
    11161 tatcgtttgt cgttctcgaa gtccggatcg cccggccagg tctgcccgcc cgccgcacgg
    11221 ctgcccggca catccagagt gaagatcggg atgtggtaca tgcgctccca tatctccgcc
    11281 cacttgatgt acgtgttgca ggcgttggta tataccgcca gcgacggcgg agggatgcgc
    11341 cccatcggat gctcgccgcc gcgcaactgc atccccacat cggccttgac gtaaccgcag
    11401 atgtcgggcg aatagccgta atcctcggcg gtgttcagat attcgtgcgc cacacgtcgc
    11461 acagcagtct gcagcgaatt gatctccggg aagacggtat ggaagtcgaa gacgcgcagg
    11521 acctcggcca gactgcccat cacgaacaca tacgccgcct tcttgccgtg ctccggcgct
    11581 tccgtgagtg atttgaacca ctcccggaag agccgcgcgc cctccgccgc tccacgcccg
    11641 actatcgtgt ctgtgctctg tctatcagtc atcgactctc ctcctcactg caccattcat
    11701 cacgcatcac gcaccacgca ccatgcctca cgcaaacagc agattctcga caaacgtctc
    11761 cagttgaatc tggaggtgat cgaaactggt catgctctct tcaaactcgc tgacgaagta
    11821 ggggatgccc tttgcgtcca acgctttgac gtaggcaacc tgctcgtcca gacccggctc
    11881 gcacatcttg gccgccgtga tgaccgccgc gctcgcatta gcctccttga tgcgctgcag
    11941 cagcatgtgc tccttcggct tgcgcagatc gtgctgcacg ggactgtacg acgatctctc
    12001 cagataggct tcggccagat tgaataacgg gtcgccttcg agcgggacgt cttccagaat
    12061 ccatcgcagc ccgatgaaca gatcgtcgtc gacgacgtag cacgattggg cgatcgcgcg
    12121 cagcaggtcg agcggcggct gctcgcagaa gccgccttcg aataccaggc ggatgcggtc
    12181 cagcggcttg gccgtgcgcg cttcgatctg cgggatgacg gctttgagca tgtcgttgtg
    12241 ctcctcgcgc aggatcatgc cgccgacggc gaccagcacg taggcctcgt cgatggggag
    12301 gagccacggc ttctcgcgct tgatggcgta cagcttgcgc agcaggcggc gattctcgtt
    12361 gtacacgcgg atcgacgcgc gcagatcgtc gtcgccgact ttgcggccgg ccaccccttc
    12421 gatctcgcgc ttgagccgcg cgtactcgtc acgcaggtaa gctgcggcgt gtgacgagtt
    12481 ggcgttctgg ggcaggtaca ggatctgaca cacataggga aagttgcggc cccagaccgc
    12541 cgccaggttg cgcgcggcgt cgcagatagg gtgggtgacg aacatgtcga tctcgatgcg
    12601 cttgctgagc gccagctcga gcgaggtctt caggatcgag cacaggtacg agccgaagcg
    12661 cgcctccgcc tgccgcgctt cgatctgggc gccgcgaatc ttcagcggca gcatgccggc
    12721 ggcgtgagcg atttcttccg ggaagtacac ttgaaagtgg cccagcacct taccacccgc
    12781 ctcgcgccag cggcgcacgg taggatattc gggatcttcc accaattccc ggcacatgtc
    12841 gagcacggca tctaacggct catagggcct gctatccagc gttctcacat tcatctgcct
    12901 cctccttacc tcccagttat cagtcatcag ttatcagtca tcagtgctcg ccgctcacgg
    12961 atcactgatt actgatcact gattactaat tactgattac tgactctcgc cccgcacttg
    13021 ccgcagaagt cgaactcatc cggcaggtac ttcgtcccgc acgacggaca ctcgcgggcg
    13081 tatggccccc acggaaactc ggacgactgg cccgccgccg cccgctcgcg cagcatgcga
    13141 tagcgcgcgg ggcgcttctc gacaaacgcg cccatacctt cgagcggctc ccacgtggcg
    13201 aaatggatcg acagccagtc gcgcgcgtgg ccaacagttt ggtgccaggc gaggtccttc
    13261 cagaaatttg cctgctgctt cgtgtagcgt gtgcattcgg ggaacttgtc gaccagctcg
    13321 cggcacaagg catcgactgc ctggtcgagc ctggataggt cgaggcaata gccgtcctgc
    13381 cccttctgcg ccttggcgat ctgatccggc gtcgcccgga cgacgaactc gccatccttc
    13441 ttcacggaag gggtcacctc gttgaccagt ccccattcca gcgcctggta gggcgggatc
    13501 cggcggttaa gcatcagcat ccatcgggcg cggcggtcgc cgacgaagat cggcagccac
    13561 tgcgtcgcgc cgccggcagc cacgctgccc acgcccgtgc cgacctggcc gatataggcg
    13621 tgttccgcga tcaccgacag atcgcacgcc aaatggcttt cgttgccgcc gccgacggcc
    13681 atgccgttca cgcgcgcgat cacaggcttg ccggtattga taatgctctc gatgtacgcg
    13741 ctgaacaggc ccatgtactt ccagtagtcg cgcggcgagc gcgtatagat ttcctcgtat
    13801 tccttcacgt cgccgccggt gcagaacgcg cgatcgccgg cgccggtata aacgatgacc
    13861 gccacctcgt cgtcgaaagc ggcttcgcga aaggcggcgg caagctccat cagtgcgcgc
    13921 gtcgaatagg cgttgtacgc ctgaggccgg ttgattgtga tgcgcgcaat ccagtcggac
    13981 ttcgcataga ggatttcggt gaattcgacg gtgcgtgtct gaatgaatga catcatcagc
    14041 ctcctgaccg gtagtcgagt agtcggtagt cgagtagtga ggtagtgacc acgtgaccat
    14101 gtgactacgt gactagggct tgctcccgtt tccgagcgtc ttttcgatga actcgttcgc
    14161 ccgcccggac agcaaccgat gatactcgcg aaacacgagc gtcgcccggt cgcccagcca
    14221 tccctccggc agcagctcgg ccggcaggtt cgggtcggat cgcgggaagg tgctgtactc
    14281 gtggataatc cagaatcggc gcacaaaaca gtcgtcaggc ctcagcgcgt tgcccgtgga
    14341 taaccgctct tcgcagctct gcaactcggg ttcccacttc cgcaagaatg ctgcgtaccg
    14401 ctgattcaga tcgccgagat tccagcagcg agccaccagg tctccacatg aaaggtcact
    14461 caaccaccgg ccgacaaagc actgcacgta gcgccacgca tggaggtcgt ccagcagggc
    14521 ccccaattcg gtatgacggt catgaggcga tacccacatc cctggagcca aaggcccgaa
    14581 ccccagccaa ctgagccgcc gccgaatcgt ctcacgcagg tcgcgcttcc tctcgggcag
    14641 cgaatacacc acaacgcgcc actgcccgtc ccactcttga cggcgcggct cgaaaatgcg
    14701 ctgagcgccc tcttcgagca ggcggcgccc cttcggggtg agcgcataca gactgttgcg
    14761 gccgcggcgc tgcggtttca gccatccctg cttgctcatg cgtgacaggg tcgatcgggc
    14821 cgcctggctg ctcaccccca gcaggccgag cagctcgatc aagctcctcg tccaggccca
    14881 cccttcacgc ggaaggacgt aatcgccaaa gagcgtgaaa atcagaaact gcgtccgaac
    14941 ctgccgtcgc ttcgacatat gtcattttga atacggtcgt ctgaaaaatg cgatatgtca
    15001 ccagtataac atcgtttttg ctgaagtcaa tatgcaaatc tcgtttcata gaaagttctt
    15061 tttctcacaa tatccacgca ggcgcgcagc cgcttcagaa gtcggcgccg agcctaccac
    15121 ttgaatggac cggctacttg ccagcaacgg ggaaagcgtc tcggcgaaga gtgaatcgcc
    15181 gacgatgaag accctttcca cggcgaccat ccggccgctg ctcgttccct gatcggctgg
    15241 gaccagccac acgggctggc gcatatccgg gggaggtact ttcgccccac tcgtgctgga
    15301 aagcaccagg gcggtattgc cttcatcgag caagcacaac gaggccgcct cggcccgggt
    15361 cagcgtgcga gcgtgatccg tcacggattg caggaggtgc cggagatcga gctgcgacac
    15421 gatctcctgg ctgaactcga aggcggaggc cagctcacgt gtgcgctgcg cgacgcggga
    15481 ctccagctcg tcacgcgaag cggcgatgtc ggcgcgcatc gtctcgaagg cgcgcgccag
    15541 gtccgcaagc tcgtcgccgc cttcgacgcg cacaggctca acgagatgtc cttcggcgat
    15601 gcgatgagcg gcccggttga gcgtcgcgag tggccgcgca aaacggcggc gcgtaaaaaa
    15661 atagccccac gtcagcagca gtaaggcggc gaccagcagg acgagctgaa tgagcaggag
    15721 ggcacgcagc gtcgcgccac ttgcctgggt ctgaacgatc aggtaagtga tcgcaacgga
    15781 gccggcaagg aggagcacga aacagaatac gattgcgccg agctggatcc gcagggagcg
    15841 gaacacggct gcctccggct gcgctgaaat aggccggtgt tgatcgtatc gcacacgcct
    15901 gaaaatcgca agccggggcg agggcgcctt cgtggttaat ctcccttgtc ttcactccac
    15961 cgtggcgctc ttgatctctt tctgcgcagc accttgcgca ccacgcgccc gacaagcaag
    16021 aagcccgttc tctcatcaag aacgggcttt gcttctatcc gtgcctatcc gtgttcatcc
    16081 gtggtttatt tactccaccg tcacactctt cgccaggttc ctcggctgat cgacgtcgca
    16141 gccgcgtcgc acggcgatag tgtacgccag catttgcagc gggatggaag ccagtaccgg
    16201 cgccagcagc ggcgcgcact ccggcacgta taacacgtgg tcggccttgc gccggatgtc
    16261 ctcatcgccg tccgtcgcga gcgcgatgac cgtaccgccg cgcgccttga cctgctcgac
    16321 ctggctgatc atcttgtcgt agacgtgatc ccgcagcgcg atgcacacga ccggcatgtc
    16381 ctcgtcgatg agcgcgatcg ggccgtgctt catctcgccg gccgggtagc cctcggcgtg
    16441 gatgtacgag atttccttga gcttgagcgc gccctccagc gcgatgggga agttgatgcc
    16501 gcgccccagg aacaggaaat ccgtataatg gtagaaatgc gccgccagag cctcgacctc
    16561 cggcgcgcgg tccatcagcc gcccggccag gtcgggcagt ttcgccagtg cgtcgaccgc
    16621 cgcgcgcagc cgctcgccgc tcaatgcccc gcgcagttgg ccgagataaa cgccgagcac
    16681 atattgatcg acctgtgagc cggtaaaggt cttcgtcgag gcgacgccga tctctggccc
    16741 ggcctgaatc aggatcgacc catcgctgat gcggtgcgcc tgagagccga cgacgttcac
    16801 gatgctccac aggcgcgcgc ccttcatgcg ggcttgctcc atcgccgcca gcgtgtcgac
    16861 ggtctcgccc gactgcgtga tggccaggac ggcggtgcga tcgtcgagga tcggatcgcg
    16921 atagcggaac tccgacccgt aatcgacctc gaccggcacg cgcgccagcg actcgatcat
    16981 atacttgccg acaagcgccg catgccacga cgtgccgcac gcgaccgaga tgatcttgtc
    17041 gatcgagcgc gccgcctcgg acgagagatt catctcgggc aggcttacta cgccgcgctc
    17101 gaagtcgacg cggccgcgaa tcgtgtcggt gatcgagcgc gcctgctcga agatctcctt
    17161 ctgcatgaag tgcgtgtact cgcccttggc cgccgcaacc ggatcgtatg gcacggtgtg
    17221 gatcggcagc tcgagcggct cgccgtcgag cgagacgaac ttcacgccgt cgcgcaggac
    17281 gacggccatc tggcgcgatt cgaggaagat catgtcgcgc gtatattcga ggatggcggg
    17341 catgtcggag gcgaggaaca tctcgccctg ccccacgccg acggtcacgc cgcccgcgtt
    17401 tccgatgcgc gacacgatca ggcggtcggg ctggtcgtgg tcgagcagca cgacggcctg
    17461 cgcgccctga agccggttaa gcgccttgcg cgccgcttcc tcgaacgtct cgccgtcgcg
    17521 caggtactgc tcgacgaggt ggacgatgac ttccgtatcg gtctcgctcc tgaagacgtg
    17581 acccgccgcc tgtagctcgt gccgcagcgc caggaagttc tcgacgatgc cgttgtgcac
    17641 gacgaccagc cggcccgtgc agtccacgtg gggatgcgag ttcgtctggt tggcctggcc
    17701 gtgcgttgcc cagcgcgtat ggccgacgcc gatcggcccg tgcatcggct cggcttcgag
    17761 cagcgatgcc agccggtcga gcttgcccgc gtcccggcgc acggcgatgt ggccgttttc
    17821 gatcacggct atgccggccg aatcgtagcc gcgatactcc aaccgcttga gaccgttgaa
    17881 gatgatcggc gtcgcgtcgc gcgagccgac gtatccgacg ataccgcaca tgggaatccc
    17941 tccttggggg attgaaccct tgcgaaggca tgccctgagc ggagtcgaag ggttcgtaac
    18001 cttcgcgagg gtttgatttt tcgccaccgc gctccgcttg tccgcgagtc gcctcgcggc
    18061 tttcgcgcct gcgcttgaac gtcacaggag ggggagacga gggtggtggc tcaccctgac
    18121 ggcatccgct gattcctcga ttttccttcg gcgtatcgca tatcgcctat cgcgtatcgc
    18181 aaagcagcct tcctggatga agcgatgcga taagcgataa gctataggcg atgagcgaag
    18241 ttagcgctgc cccacggcag gaaccgtctc ctcgtcaacc gggccgccga gcgacggcct
    18301 cagtccatgc gctgtctccc cgcttattcg tccgagcgat cacctcctta tccggtgtgt
    18361 tacgacaaca ttctaacaca accccggcgt ctatgcgtcg gaaaacggtt agagaatcga
    18421 ccccgatttc ttgagttccc tgatcacctt cagttccctc agcatgtgcc aggcacgcca
    18481 ggaactgaag gaactcaagt cagtccggcg cagcctgcag gaaccgcaca taccgcgccg
    18541 tcgccgcgtc caggttggag tagacaccgc gctgctcggc cggcaggagg gaagccagtt
    18601 gatacatcgc ctcgtcggtc atctgcttga gcgtctcgcc gctcaggcgc tcctcgtccg
    18661 cagccagaac gaatgggcgg cccacgcgca cccggatcgt cgtgcggcgc aggcgcttga
    18721 tattgcggct ccagcgatcg ctgccgctga acgccacagg cacgatcgga gcgcctgcct
    18781 gcgacgcgat gtacgccagg cccggctgcg cttcgagaag ggtggccgtc ttactgcggg
    18841 tgccttcggg cgcgatcaga agaggcaggc cctgatcgag cacgtcaaac gccgcctgaa
    18901 gcgctttgcg gtcgacgtca ccccgactca ccggtatcgc gtcaaaggtg cggagcaaag
    18961 ggccggtgag ccagtgttga tacgcctcaa tcttcgccag ggcgatgacg gagcgtggaa
    19021 agcggccgct gacgatcaca gggtccacga aggcgatatg gttgaacatc gcgatgcagg
    19081 caccgcttgc aggaaaatgc tccaatccct cgatagtcag gcgcatcgtc accttgaagg
    19141 ccacgcgcag gaacaacgtg tagaaccggc gcagcaggct cggcttgtag ttctggtatt
    19201 tgctcgcgcg gccggcgcgc ttgctgcgaa atcgtccccg gcgcttgacg tcggtcgtgc
    19261 tcgtcatgtc accttcgctt cggcgaagcg tctcacaatc tcctcggccc gatcgaccat
    19321 cgcgctcatg tccagcttcg tcgtgtccag agtaatcgcg tcaggcgcgg cgaccagggg
    19381 ggagatcggc ttctcccggt cacgcacgtc ccggcggcgc atttcgtcga gcacatcctc
    19441 gtaagtggcc tgctcgccgc gccgcagcag ctcggcgtgc cggcgctgcg ctcgcacctc
    19501 gagcgacgcg tcgacgaaga gtttcagatc ggcatcgggg agcaccaccg tgccgatgtc
    19561 gcgcccgacc atgacgactt ttccccgctg cgcgatgcgc cgctgccggt cgaccagcgc
    19621 gcgccgcacg ccggcgaccg ccgaaacggg agaaacgatc gcgtcgaccg ccggggagcg
    19681 aatgtcccac gtcacatcac gcccgtcgag caggacggtg tcgagacggc catcggcggc
    19741 ggtcggctca accacgtcga tctgcacacg ccgggcaagc tcggtcagcg aaacatcgtc
    19801 gtcgacgctg acgccgtgct ccagcgccgc cagcgacacg gcacggtaca gcacgcccgt
    19861 atcgaaatac aggtatccca ggcgctgcgc gagcagatgc ccgagcgtgc tcttgcccga
    19921 gcctgccggc ccgtcgatcg caatcaccga gggtttcatc gcatatcgca tatcgcatat
    19981 cgcgtatcgc gtatcgctga tggctgatgg cagacagctg atggcttacg cagatggtat
    20041 atcgaaattc gcctcacgct tcacacctca cgttttactc tttacgcttc acgactcacg
    20101 ttttacgctt cacgttttac atttcacgct tcacggctct cccgaatcga ctctttcttt
    20161 tcaccactgt tggtccggac tggctcttct cgaccaatcc gcgcagtgag cgaatctctt
    20221 tcgcattcag gaagcgccac ttgccgggct tgagatcgcc catgtcgatc gggccgatac
    20281 ggacgcgcag caaatactgc acggggtgtc ccaacagccg cgccacctca cggatctggc
    20341 gcttgcgacc ttcgcgcatg acgacgcgta gaagcgtgcc gccgccttcg tgccggacga
    20401 cttcgacgcg ggccggagcc gtcttgtagg cgccttccgg gtccgggaca accacgccgc
    20461 gccgccagac gtcgagcgcc ttggcgtccg ggcggccctt cacgaagacg agatactcct
    20521 tctcgtgctc gtagcgcgga tgcgtgagca cgttcgccag ctcgccgtca ttcgtcagga
    20581 gcacgaggcc ctcgctgtcc gcgtcgagcc ggccgacggg gtaaatctgt ccgtcgtagt
    20641 ccaccaggtc gcgcaccgtc gtacgcccca actcgtcttc attcgtcgtc accacgtttc
    20701 gcggcttgtt gaggatgata tacacaggcg gcgactcgat cttcaggcgt tgcccgtcga
    20761 ccgcgatcgt gtccgccgcc ggatcggcct tgtcgcccag cttcatgact ttgccgttga
    20821 ccgtcacacg acccgcggcg acgatctctt cgcacgcgcg gcgcgagccg tagccggcgt
    20881 gggccaggag cttttgcagt cgttcggatg gcattcgtcc ctcgtgcagg acgattatac
    20941 agcaaaacgt gaccggcact tcggaagtgc cggtcacccg ttactgcaaa gatgcgccat
    21001 cagagctgta gtgcactcgc ccgaacgagc ttattcttta cgtacgcgct cgtctgagca
    21061 ccgcgtatag cacgccagcg ccgagcgcgc ctgctactgc gccggaaatc acaccggaag
    21121 ggaaaaatcc tccaagcact acggccacgg cccaaccgac ggcgatgcca atgacctgcg
    21181 accaccgaat ggccggctcc acccatcgca gcaccaatcc ggtggacgct ccaccgacca
    21241 gacctgcgat ggcgtagctg agaaaaaaag agagagccat cgacacacct ccccgacgca
    21301 gtgtcgtcta aaccaagcta caggaagata agccagatcc accacgcgat agcccagccg
    21361 gcggcgatca agaacacgtg cttctcttga agtgctttgg ttgtccaccg caacgaagcc
    21421 ccggtgatga aaccgccgat ggcgccccta agcacagagg gggggaagat cgcccaagcg
    21481 atgatccacc cgaccggaat gagcagcaac atcggccact ctggctgtgg gcgcggctca
    21541 acaccggccg cctgcacgcg taagccgacc tctgcaccag tcgaccggcg tcgcatgaag
    21601 atgtccgctg tctgagtgac cgctggcgcg acgcgcaagc cggactctga tttgagacca
    21661 cttacctgat tgagatgcca gaacatcacc ccgccgccaa tcgcaccgac gatggtcccg
    21721 gtgacagcca tggcagaaac cgcttcttca gcactggcat ccgtcatagt gaccaatacg
    21781 atggaccaga tggaccatcc caatgtccac gccaggcccc aaccaacagt aatcactcga
    21841 atttgaatta acggcttgat tcgcttaagc acccagccgg taatcagccc gccgatcgtg
    21901 ccgctaccgg ccccaaacac agcgccggag gttagattgg cttcttgagt tgtcaggccg
    21961 gaaagaacaa tgagggaaat cagcaaggca accgcgaatc caccggcaac tacgccaatt
    22021 atccaaccca ctaatgccac aagcgcctga ttccatttaa cgattacccc ctggtggcgc
    22081 aacgccaaca cgataataac cgttagagga acaggtacgc cgaagacgaa gctggcaatc
    22141 acgtcctcag acgacagggt tcgtgcagca atagcccagc tcaggcccaa gcctatcgtc
    22201 gtcaacagca cgggtatgcc tatggcattc gtcagcccag cgccgctgtc gatctcgacc
    22261 gtgcgcccgg tttgcatctc aggtgcaatc cgttccgcct gggtcttttg tgcggcgaga
    22321 cgttcagctt ctgctgtctg ttgcgccagt cgttcccgct caaccttctc tcgtgcaaca
    22381 cggtctgcct cagccctctg tgcggccatc cgtgcctgct ccacgcgccg cgcctctcga
    22441 gctaggcgtt cctcttcggc cttcttcgcc gccaggcgat cggcctcggc tttgtcacgc
    22501 gcgatgcgcg cagagcccgc ctgcgcggcc tcacgtgcat gctgctcatc cgcatacgta
    22561 gctagaattc cggcagccgc cgtcgacacc cggcggctat cgtcgtcggc caggcgcgtc
    22621 aaggcgtcgc gtgctgcctg cgccagaccc aactggctgc cgtgcagcag ccggtcgagt
    22681 tcgcgcaccg cgccctcgcg cacccacgga cgcgggtca
//
LOCUS       DBNHXB010000295         3820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1251670, whole genome shotgun sequence.
ACCESSION   DBNHXB010000295 DBNHXB010000000
VERSION     DBNHXB010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1251670"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2451
                     /locus_tag="AC162G_11855"
     CDS             <1..2451
                     /locus_tag="AC162G_11855"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076559.1"
                     /translation="GEYVRFGYHRAFANNHQLWVHDPLARMDDGLFIGLQHSQVSSAI
                     PTTTFSFQVDFYGIDDNAWLAESTSSLQVPAGASRTFDVTLSVPGDADAGIYEAALYV
                     DDPGDAGHLAHTTVVPVVVNVAEDFTPGDIVTLGGMDAFDADAMYNNAGVQGTFDWSW
                     RAESGDWRFFMLDVPVTPTVGTAWLIRDVWDDGYPTDIDTLVMGRTRDAFSNGPTAGN
                     PSDGLFDLRDQLCAIFGNAVFGGLGCDTQDSFYGGFVTQDPAYYGPYTEATVGGSANT
                     YLGSGKWVFDTATGGAEEWVVAPIQEGLHIVAEHNVNFSGAKFTVPYTKTVSSINLTP
                     AAIEITTSVNSGAVPITFTSGLTLPAGLAAAGFGLGAPTVYVTQTVTQDDDQDPSTDT
                     FNTANGFTNYDFTVENGASILAETFDATTNDIDMYLFYDDAGDGFQYSDLVASSTTPT
                     SDESVAVVRPGDGNWRLSIHGWDVTGGGTYKLRLTVVQGFDLSVTGVPTGTVNAGAPV
                     VLTVNYAKIMQPGSTYEGLLLLGPSVAPNALAVPVIIHRTGTGTPNLTTSTKTADKSV
                     VPVGAQVQYTINVVNTSPVTATTAAFVDLIPAGTTYVAGSLVGTNAVFTPTLGGTLGP
                     AIVWTRSVPANGTRTITFRVVNGAPAGTTITNTAVISDFANAPELGGVTLASVTYQSA
                     SPLAASTKSATPQVGNGGTLLYTIVLSNSSSVPVDAQVGDAMPTGVTATGATAGATVS
                     EDGTNVFWGGTVAANSSYSIAITARVNISATLPALLSGVPVTNTVSINDGGSTFTRSA
                     VTEVGPMQTYLPIIMKNF"
     gene            complement(2506..2973)
                     /locus_tag="AC162G_11860"
     CDS             complement(2506..2973)
                     /locus_tag="AC162G_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076560.1"
                     /translation="MNERLWILMIVCLAACSSPPGTPESQVSPLQLPQLPVTNYPLTG
                     TPDSQVSPLEAPVTTSPLITPASGTLSPAEKAVVDLAERLAIDPASIQIERVEPDEFP
                     AQELGCAEPASKELSGDRPAFVTGQAIWLRVGDALYEYHSHGSQLVFCGMRQE"
     gene            3060..3236
                     /locus_tag="AC162G_11865"
     CDS             3060..3236
                     /locus_tag="AC162G_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076561.1"
                     /translation="MCGAPRLPLVFSSPGYYAITATVEHAGLDLGAQNNSAGLTALAA
                     RVRAFVPIVLRETQ"
     gene            complement(3368..>3820)
                     /locus_tag="AC162G_11870"
     CDS             complement(3368..>3820)
                     /locus_tag="AC162G_11870"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HIR5076562.1"
                     /translation="LGVVVLDKRVRMETYGALPEQDVLDVMATERGLQDVTFTAVGYG
                     LQESFPDAASWKEHNVRVRMVSYPHLIQINGGLVGDFSLLLSNNHATGGTCFGDSGGP
                     NFIDKTNIIGGVTSFGLNGNCAGTGGVYRVDKADDLDWLYGTFGDHLP"
BASE COUNT          713 a         1216 c         1185 g          706 t
ORIGIN      
        1 ggcgagtacg tccgctttgg ctatcaccgc gcgttcgcca acaaccacca gttgtgggtg
       61 cacgatccgc tggcgcgcat ggacgacggc ctgttcatcg gcctgcagca ctcccaggta
      121 tccagcgcga tcccgacgac cacctttagt ttccaggtgg acttctacgg catcgacgat
      181 aacgcctggc tcgccgaatc gacgtccagc ctgcaggtgc cggcgggcgc gtcgcgcacg
      241 ttcgacgtaa cgctgtccgt gcccggcgac gccgacgccg gcatctacga ggccgcgctg
      301 tacgtcgacg atccgggcga cgccggccac ctggcgcaca cgacggttgt gccggtcgtc
      361 gtcaacgtgg ccgaggattt cacgccgggc gacatcgtca cgctcggcgg catggacgcc
      421 ttcgacgccg acgcgatgta caacaacgcc ggggtgcagg gcaccttcga ctggtcgtgg
      481 cgagccgagt ccggcgactg gcgcttcttc atgctcgacg tgccggttac cccgaccgtc
      541 ggcaccgcct ggcttatccg cgacgtgtgg gacgacggtt atccgaccga catcgacacg
      601 ttggtgatgg gccggacgcg cgacgcgttc tccaatggtc cgacggcagg caatcccagc
      661 gatggcctat tcgatctgcg cgatcagttg tgtgccatct tcggcaatgc agtgtttggc
      721 ggcctgggct gcgacacaca agactcgttc tacggtggct ttgtgacgca agacccggcc
      781 tactacggcc cgtacaccga ggcgacagtt ggcggcagcg ccaataccta ccttggcagt
      841 ggcaagtggg tgtttgacac ggccaccggc ggcgcggaag aatgggtcgt cgctccgata
      901 caggaaggtc tgcacatcgt ggcggagcac aacgtgaact tctcgggcgc caagttcacc
      961 gtgccgtaca ccaagaccgt ctccagcatc aacttgacgc cggccgcgat cgagatcacg
     1021 accagcgtca acagtggcgc ggtgccgatc acgttcacca gcggcctcac gcttcctgcc
     1081 gggctggcgg cggccggctt tggcctgggt gccccgacgg tgtatgtgac tcagaccgtc
     1141 acgcaggacg acgatcagga tccgtcaacc gacacgttca acacggcgaa tggcttcacc
     1201 aactacgact tcaccgtcga gaatggcgcg tcgatcctgg cggagacctt cgatgcgacc
     1261 acgaatgaca tcgacatgta cctgttctac gacgacgccg gcgatggctt ccaatacagt
     1321 gatctggtcg cgtcgtccac gactccaacg tcggatgagt ccgtcgcggt tgtccggccc
     1381 ggagacggca actggcggct gagcatccac ggttgggatg tcacgggcgg gggcacgtac
     1441 aagctgaggc tcacggttgt ccagggcttc gacctcagcg tgaccggcgt gccgacgggg
     1501 acagtcaatg ccggcgctcc ggtggtgctg accgtcaact acgccaagat catgcagccg
     1561 ggcagcacgt acgagggcct gctgctgctt gggccgagcg tcgcgcccaa cgcgctggcc
     1621 gtgccggtga tcatccaccg taccggcacg ggcacgccca atctgacgac gtccaccaag
     1681 acggcggata agagcgtcgt cccggtcggc gcccaggtac aatacacgat caacgtggtg
     1741 aatacctccc ccgtcacggc gacgacggct gcgttcgtcg atctcatccc ggccggcacc
     1801 acttacgtcg ccggctcgct ggtggggaca aacgcggtct tcacgccgac gctgggtggc
     1861 acgctcggcc cggccatcgt ctggacgcgc agtgtgccgg cgaatggaac acgcaccatt
     1921 accttccggg tcgtcaacgg cgccccggcc ggcacgacga tcaccaacac cgcggtgatc
     1981 agcgacttcg ccaacgctcc ggagctgggc ggcgtcacgc tggcctcggt tacttaccaa
     2041 tcggcctcgc cgctggccgc ttcgaccaag tccgccacgc cgcaggtggg caatggtggc
     2101 acccttctct acactatcgt gttgagcaat agcagctcgg tgcctgtcga cgcccaggtg
     2161 ggtgatgcca tgccgaccgg cgtaaccgcg acgggcgcga ccgccggcgc gacggtgtct
     2221 gaagatggca ccaatgtctt ctggggcggc acggtggcgg ctaactcgtc gtactcgatc
     2281 gcgattacgg cgagagtgaa tatcagcgcc acactcccag ccttgcttag cggagtgcct
     2341 gtgaccaaca cggtgtcgat caacgacggc ggcagcacct tcacgcgctc ggcagttacg
     2401 gaggttggcc cgatgcaaac ctacctgccg atcatcatga aaaacttctg agcgcagttg
     2461 gctgtagggg aagacccgac aggtctctag agacctgtcg ggtctttatt cttgtcgcat
     2521 accgcaaaac acgagttggc tgccgtggct atgatactcg taaagtgcgt cacccacccg
     2581 cagccaaatc gcttgaccgg tcacgaaagc cggccgatcg ccggataact ctttggatgc
     2641 cggttcagcg cagcccaact cctgagccgg aaattcgtcg ggttccactc gttcaatctg
     2701 aatcgaggcc gggtcgatcg ctagccgctc ggccaaatcc acgaccgctt tctcggctgg
     2761 gctgagcgtg ccactcgccg gtgtgatcag cgggctagtg gttaccggcg cttccaacgg
     2821 cgacacctgg ctgtcgggcg tgccagtcag tgggtaattg gtaactggta attggggaag
     2881 ctgcagcggc gacacctggc tctcaggcgt gccaggcggg ctactgcacg ccgccaggca
     2941 cacgatcatc agaatccaga gccgctcgtt catcagcgca ccccctgttc ttaagacgcc
     3001 gctgacatcg gatggttccc ttacaacgaa caggtggtct actatcatga ggctatagta
     3061 tgtgcggcgc acccagactt ccgttggtct tctcgtcgcc gggttactac gcgatcacgg
     3121 cgacggtgga gcacgccggc ctggatctcg gtgcgcagaa taacagcgcg ggactcacgg
     3181 cgctcgccgc gcgcgtgagg gcgttcgtgc cgatcgtctt gcgcgagact cagtaaatcc
     3241 aaattcggaa gatggcacga tgcaccggat ggacaacgga gttaagagaa ctcagggatt
     3301 ccgaaggcga ttcgaaatcc ccgagttcca tggcaggtgc tggagggcgg atgcccgggc
     3361 tttgccctta cggcaggtga tcgccgaatg taccgtaaag ccagtccagg tcgtcagcct
     3421 tgtccacgcg gtacacaccg ccggtgccgg cgcagttgcc gttgaggcca aaggaagtga
     3481 ctccgccgat aatgttagtt ttgtcgatga agttggggcc gcccgagtcg ccgaagcagg
     3541 tgcctccggt agcatggttg tttgacagta gcagggagaa gtcgcccacc aggccaccgt
     3601 tgatctggat caggtgggga taggacacca tacgcactct aacgttgtgt tccttccagg
     3661 aggctgcatc agggaaactt tcttggagtc cataacctac ggccgtaaag gtgacgtcct
     3721 gcaagccgcg ttccgttgcc atcacgtcta acacatcttg ttcgggcagc gcgccgtacg
     3781 tctccattct gacccgctta tcaagcacga cgacgcccag
//
LOCUS       DBNHXB010000296        24635 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4439015, whole genome shotgun sequence.
ACCESSION   DBNHXB010000296 DBNHXB010000000
VERSION     DBNHXB010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 24635)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24635)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24635
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4439015"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2546)
                     /locus_tag="AC162G_11875"
     CDS             complement(<1..2546)
                     /locus_tag="AC162G_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5076563.1"
                     /translation="MNPSTHYRPALQLALTALLAALALVLALSFAFADDAGVSSGSPL
                     PGRAERQDHGTGQYPRGQSAPSNLTPIDGPPAPGKIDPPLLKRMLAAAPGEQIPIIVE
                     MRQQADLDSAPLRAAGISQAEAVVHALRTTAAHSQRGVRAQLAVMEKAGRASQVRSFW
                     VFNGLASHVAAGDILALAARDGVGLIREDRFRQWVTAPPSSLPPPTSTSNSNSLTLTL
                     EWGLRKIRADEVWAALNVTGTGIVVANMDTGVDWQHPALITAYRGYGKGLAGHAGNWL
                     DATDDATVYPVDPHGHGTHTMGTIVGADGIGVAPGARWIAARVLNSAGFGYDSWIHAG
                     FEWILAPDGDPNLAPDVLNNSWGSDVSASTAFQSDLHALRQAGIFAVFSAGNNGPDAG
                     GIGSPASLPEAFAVGATDDEDEVAVFSSRGPSPWGEVRPHVAAPGVNVRSSVPGGIFD
                     RYSGTSVAAPHAAGTAALMLAANPSLTITGTAFVLTGAAVPLSTTIPNNDSGYGRVDA
                     YAAVQAVASMGTLSGTVARSDTGAPIAGATVAAVSNAGSGTASTGANGQYVRGLAASI
                     YTVDASAFGYVTATQAPVFVITGAATLLDFSLDPVPSGEVRGSLTDAESGQPISGTIV
                     VAGTPVTASAAARGVYSATLPGGTFELRAVAWGHRALTATVTVTPGAAVTRDFSLPPA
                     PTILLVDSGPWYYGSQIGYYRAALEALGYPYAEHRVKRLPADVPQWSTLAAYDAVIWS
                     APQDAPGFIGAGDAISSYLSAGGSLLLSGQDVGFWDGGLATNWSSYYHDRLKAIAVED
                     DSGSRTVSGVGVFAGLAASIAGPGGADNQWYPDAIGSAEPDLTADAFTYA"
     gene            2946..3548
                     /locus_tag="AC162G_11880"
     CDS             2946..3548
                     /locus_tag="AC162G_11880"
                     /inference="COORDINATES: protein motif:HMM:NF018822.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HIR5076564.1"
                     /translation="MQRAAKGMASQNTLRFTIDIAGTPAMLNPALGLALRSGEGVFAR
                     PDRMGVHLKLVGPVAIEADMIALGDEQYITNFLSKEWEPLPAEIGFNPAVMFHPEFGL
                     EKTLEGGLDDAELVGVESIDGAQAYYVKGRIEGSRLWRMSGGLIGSSTVDVEVWVDPQ
                     TFAVRKIVLVDPTIDPGNPSIWTMTFSKFGEPVTIEAPDV"
     gene            3608..5164
                     /locus_tag="AC162G_11885"
     CDS             3608..5164
                     /locus_tag="AC162G_11885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5076565.1"
                     /translation="MPYSSRLPRPNAIIALVSVAIFVGAMDLTVASAFLPQVVVDFEL
                     TVDQSALAGWVVTMYLAAYAVSMTFAGRLSDLYGRRAAYLACLAIFVAGSVMVAVTQR
                     APFAQSLEWLVMSRVVQALGAGAMVPVSMALAGDLYPPEKRATPLGAIAAVDTAGWVV
                     GHLYGGIMVRLFGDWRIIFWLNVPIGVLAFILCWRALRDAPQSKGQGGLDWIGVLLIG
                     GALVAFNISLGSGAESGAVSPFVEQTAPADQTPLLLAAAVLSCAFIVWELRARDPLLD
                     MRLFRNRTFTAASLTNLLVGFVLIVALGLVPLFVNVVVASSQPGLTLEQILSDGAWYT
                     GWVLSGLTLTMAIVSAFAGRIVERRGYRLPASVGLALGAIGFWIASRWTSGTTYFEML
                     PGLMVAGAGFGLVLAPVATAVINVAPAQERGVASALVIILRLVGMSLGGSTALAWWTQ
                     RVQTLTANLSAGSSLFSGDAFEVFRTATAQAVGESFILFAVLPCLAGLLPASQLRSED
                     ELPSGSFPKA"
     gene            complement(5638..6093)
                     /locus_tag="AC162G_11890"
     CDS             complement(5638..6093)
                     /locus_tag="AC162G_11890"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5076566.1"
                     /translation="MARFSNNLIIGRPINEVFRFVADFENMPKWNYYVVEVNKVTTSP
                     IGVGTVFRQVRKTDTQRYKIVEFEPNRKVVVETLPPAPALQMRFTFEPAGGGTRLTDE
                     WELKGGLIGLFGSFAAGRVKAAVAENLAKLKQLLETGEVRLQDGRVERI"
     gene            6439..8532
                     /gene="acs"
                     /locus_tag="AC162G_11895"
     CDS             6439..8532
                     /gene="acs"
                     /locus_tag="AC162G_11895"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetyl-CoA synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIR5076567.1"
                     /translation="MSEREFMRVSEAEIAVHWQEEEYYHPSAQFIAQANMADPEVYER
                     FSLENFPECFKEYADLLTWYQYWHTTLDTSDAPFWKWFVGGKINACYNCVDRHLPKYK
                     NKAAIIWVPEPEDEPHVALTYRELYVRVNEVAAVLRDFAGLKAGDRVTLHLPMVPELP
                     ITMLACARLGVIHSEVFGGFSGKACGDRIADSGSHVLITMDGYYRSGQLGDHKEKADV
                     ALKAAEEQGQTVDKVLVWRRHRGKYVSQTPMRAGRDYFMDDLIRDYGGKRVDPVPMPA
                     EAPLFLMYTSGTTGRPKGCQHSTGGYLAYVAGTSKYIQDIHPEDVYWCMADIGWITGH
                     SYIVYGPLALAATSVIYEGVPTYPDAGRPWRIAEQLDVNIFHTAPTAIRMLRKAGPDE
                     PTKYHYQFKHMTTVGEPIEPEVWKWYYHVVGKGKAVIVDTWWQTENGGFLCSTMPALH
                     AMKPGSAGPGVPGIHPVIYDENGNEIEPGSDKAGNICIRNPWPGEFQTIWGDRERYVA
                     QYYAKYCKNKNSKDWRDWPYFTSDGAMQAKDGYVRILGRVDDVINVAGHRLGTKELES
                     ACLTVDKVAEAAVVPAVDEVKGRVPDIYVSLKPGYPSSKETGYEVIRALEMIIGKIAR
                     PRHVYIVPDMPKTRSGKIMRRVLAAISNNMDVGDVTTLANPEVVEEIRRMVQGETVIV
                     TQEVPEDIRQFGEER"
     gene            complement(8650..9177)
                     /locus_tag="AC162G_11900"
     CDS             complement(8650..9177)
                     /locus_tag="AC162G_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076568.1"
                     /translation="MRKRLFSILTTLVALSLMTAPVFAGRGHFVGAVSFSLGESLTAS
                     GVLAGYGNRDAIVELVGSGIGTATCANKGGNEAPGQNPIQVEVSGIQEVSASLIENGS
                     APFTVVTDAPPMPSAREAGCANNNWKVTDLFVYWDYARMTVTDAASGEVLLEQEFTCV
                     TSGPPNESVSCTPVP"
     gene            9561..12899
                     /locus_tag="AC162G_11905"
     CDS             9561..12899
                     /locus_tag="AC162G_11905"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5076569.1"
                     /translation="MTEREQMEQAIRALEAQRAMLGDAVVDTALAPLREKLAALEAQA
                     EARERQQRKQVTVLFADVSGFTAMSEKVDAEEMSATMNALWSRLDKAIVDHGGMIDKH
                     IGDAVMALFGAPTAREDDPERAIRAALAMQRELAEFRAHLDSMLVARPGAGVSLPEIA
                     MRIGINTGPVLLGQVGTTSEYTAMGDAVNVANRLEHAAPVSGILISHDTYRHVRGVFD
                     VLSLEPISVKGKSEPIQVYVVRGAKPRAFRVPTRGVEGIETRTIGREAELQLLRSAME
                     TARHSRRTHLVSVVAEAGVGKSRLLYEFNNWLELLPERILLFKGRASQEMINLPYSLI
                     RDVLAFRFEIQDSDRASTARDKIERGIAEFLGPVAVEKAHFIGHLVGIDFSHSPYLQG
                     ILGDARQIRDRAFHYIGQLFEAVTREHAAAILLEDIHWADDGSLNLIEYLARERPDLP
                     LLIIGLARPTLFERQSAWDAELPSHIRLDVRPLTEPDCRRLVAEILRKVPMVPAELDD
                     LIVSRAEGSPYYVEELIKMLIDDGVIVAREDQWRVELERLAAVRVPATLTGVLQSRLD
                     SLPPPEREVLQQASVVGRVFWEEVVARLHHPDTPRIELPATASERLAALSRKELVFHH
                     DPSAFAGMHEYIFKHALLHDVTYESVLMRLRRAYHAQVAAGLIELSGERVGEFAGRIG
                     EHFERAGEWLQAAEWYGRAGKQARDTYAPEAAISYYRKALEFAKEFPATASRFDLYAG
                     LGEALVDQARYAEAMDTFTAMREAADAAGDRLAEARAWYGLATAQVNHGDHHAAVVSA
                     ERAEALAQAADARLELAKALEIKGGGLYRLGDAEAARAAVEQAIVIATELDARRPMAS
                     SLNLLGLVHNMLGRYELAERHFEKAIAICRELEDRRRVMSLVNNLGVIAEARGDYAAA
                     LERYRNALQIARDIGHRDGEFVFLNNVGAARVGLGEYVSAEADLRHVIQMAGRAGPGM
                     LSNAHGFLAEACLGQGKVAEALEAARLALGLALESDEQEGIGAAWRTLGQVVAQWPEP
                     VAIGAGPDPSRRVDAVACFAESLRVCTETGMEGERARTLKAWATHELQRGDRARGAEM
                     WQEAHATFARLGADREAERMSRMP"
     gene            complement(12905..14149)
                     /locus_tag="AC162G_11910"
     CDS             complement(12905..14149)
                     /locus_tag="AC162G_11910"
                     /inference="COORDINATES: protein motif:HMM:NF017984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5076570.1"
                     /translation="MHTLSPTLARRLAITKQRLADPRPAVDPPGILEVVRDLGCLQLD
                     PISAVARSHQLVLWSRLGPYDLDDLDTLLWKDRSLFEYWAHVASIVLTEDYPIHNLLM
                     RRYAAGTPKTPRAKKLRQWLKDKAGLRRHILGELHRNGPLPARYFEDEARSDWYSTGW
                     TSGRNVTEMLDYLWTKGQIMVAGRSGLQKLWDVSERCLPKWTPREKLAEREVVRRAAQ
                     KSLRALGVATPRHIGLHYIRGRYPDLKSVLSELQSDARIERVEIRDGGQVWQGEWYMH
                     AEDLPLLERLEIEHWEPRTTLLSPFDNLICDRARTKQIFGFDYTVEIYVPPPKRKHGY
                     YVLPILHGDRFVGRIDPKMDRTRSVLAINAVHAEPDAPNSRTVSRAVAKAIEELAEFL
                     GAKDIEYDRRRMPSSWKRDLTA"
     gene            complement(14214..14408)
                     /locus_tag="AC162G_11915"
     CDS             complement(14214..14408)
                     /locus_tag="AC162G_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076571.1"
                     /translation="MSASVWLTGWLLDQFALDPRAFSLLLAASSVVPLGVWAIASRPH
                     ARVARSRRPAAELGDGTPTL"
     gene            14511..16112
                     /locus_tag="AC162G_11920"
     CDS             14511..16112
                     /locus_tag="AC162G_11920"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR5076572.1"
                     /translation="MNQLIAALPSLESRPDSDPEITLVTDDSRRVAPGALFVAYRGVS
                     VDGHRFVPDAIARGAVAVVGERGDLTGFQNLSGLDVLYLHVRSGREALAYLHAAWNDF
                     PARRLIMIGVTGTDGKTTTTNLIYSILKVAGVRAGMISTVNAVIGDEMLDTGLHVTTP
                     DADEVQGYLRRMVDAGLTHCVLEVTSHGLEHHRVDACEFDVAVVTNITHEHLDIHGSV
                     EGYLAAKARLFESLAEAYDKGVPKTAVLNVDDASYGYLRPRVRGRVLAYRIVRHGDTE
                     VTETHGENAMSAWRDRVIQATHVVYEPDAIRFVAQGDDFSVPIETSLVGEYNVSNCLA
                     AIAATASALGVPPEVTARGIAGLKSVPGRMERVDEGQAFIAVIDFAHTPNALDKAIAA
                     ARTMTQGQVIVAFGSAGLRDREKRRLMGEVAGRYADKVVITAEDPRTESLDAIMDATA
                     RAVAAQGRAEGVDFWRVPDRGEAIAFACSLARAGDVVIACGKGHEQSMCFDDIEYPWD
                     DREAMRAALRGEPLRTLPTAQPRVG"
     gene            16149..17111
                     /locus_tag="AC162G_11925"
     CDS             16149..17111
                     /locus_tag="AC162G_11925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5076573.1"
                     /translation="MLDLAVVIVSYTVRDLLRDCLRSALANEGLAFSICVVDNDSPDG
                     SADMVEREFPQIHLVRNSNSGYAAGNNLGLRYFGFGETPNPTSNLQLPTSNLQSPISN
                     PQVPTATSPRYALLLNPDTLVPPDAFARVVAYMDAHPDIGVLGVKLVRADGSLDLACR
                     RSFPTPEVSFYRLAGLSKLFPRSPRFGRYNMTFLDPDQHAEVDSVVGAFMLVRGEAIA
                     QAGLLDETFWMYGEDLDWAYRIKQRGWKVLYYPEVTVLHLKRSSSAGSARARYEFQRA
                     MWVFYCKHYRAATPRWLDALVRLGLGLRGGRRLLAEMSRDGGRQ"
     gene            17108..18526
                     /locus_tag="AC162G_11930"
     CDS             17108..18526
                     /locus_tag="AC162G_11930"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HIR5076574.1"
                     /translation="MSKGKVRAFFGITLVLSDILMAAMAFVFSYLLRLSLALPNAAVS
                     VAPINEYLGLMVVFVGSMVIVFFFARLYHQGRAISRVDELYTVFGAVSISSMMSVALS
                     VLLFKNSAFELDFPRAMVIYAWLLTIVLVTLGRLLHHRVQGQLQARGVGRDRVLIVGA
                     SETGNMILQKIKWSPQLGYQVVGLVTRNPDFALSEVFGTTVVGAVDDLPRLIQEHQVD
                     EVIIALPEATHTEVLDLVAKCDHGRINVKVFPDVFQIIASQVSIDDLGGLPLLSMRDV
                     RLRGWHVTLKRGFDFVVSAIVLVLLSPLLLFVALLVKLESPGSVFYAQERMGLDARPF
                     MMLKFRSMRRDAEANGPGWTTPDDPRRTRLGAILRRLNVDELPQLINVVVGEMSLVGP
                     RPERPVYVEQFKKTIPRYMERHKEKAGITGWAQVNGLRGDTSVAERTKYDLWYIENWS
                     FLLDLKILIRTFFRVINDRNAY"
     gene            18668..19255
                     /locus_tag="AC162G_11935"
     CDS             18668..19255
                     /locus_tag="AC162G_11935"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR5076575.1"
                     /translation="MQDAPILKLTMRQGPRPDQTFELHKDVYTLGREAGNDIIINDPQ
                     ISRHHARLTLQGGSYVLEDLGSTNGSFVNGRRITGPTALSPGDMVGFGDTVVLSVSGA
                     MDAAATQVGRPPQTAPSMQAARPSAPTPSQPPPATQAGVPQNTQRMLVIGCAALTLVV
                     IICMMALVAWSFLDCPSFEAFWESLPILGSIQVSC"
     gene            complement(19295..20314)
                     /locus_tag="AC162G_11940"
     CDS             complement(19295..20314)
                     /locus_tag="AC162G_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5076576.1"
                     /translation="MANLQSLISLTPMPRPILDHLYQITLPTPFPVGPVNVYLATDEP
                     ITLIDTGPRDDAARQALGEALHTLGLKRGDIRRILVTHAHSDHCGLAAEIARDGDGSE
                     VWTHPYNRMWLEDYETERARWLAFYSQLMRESGVPPDEQERMAVARRGVGRFTEAVRI
                     HRELGEGDRVDFAGKTWRVLHMPGHAGGMLCFFEPESRLMLSSDHLLRDISSNPVVEP
                     PPHPGAPKPHRLVEYVREMQRAADMHPSIAWTGHGEPITDVDKLVRQRLMFHSRRAQR
                     IVSLLENGEMTAYEVSGPMFGRLGPIDSFLALSEVIGHLEWLEEQRRVESFRRGETVY
                     WRSAE"
     gene            complement(20373..20837)
                     /locus_tag="AC162G_11945"
     CDS             complement(20373..20837)
                     /locus_tag="AC162G_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR5076577.1"
                     /translation="MSDYQPRGLYFEDLTTGLKVTSAGRTISEADIMQFAGLSGDWNP
                     MHVDAGYAKQSIFGERVAHGLLGLSIASGLAMQLGFLDRTVEAFTSLDWKFRAPIKIG
                     DTVHMTAEVTKTRAMGTSGGFVTFNVVVKNQRGETVQRGEWTIVVKGKPKVE"
     gene            21049..21474
                     /locus_tag="AC162G_11950"
     CDS             21049..21474
                     /locus_tag="AC162G_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076578.1"
                     /translation="MDWLPFAVGAAVALATADFSVKLAAGQIGSQIGALIYSLTTLVI
                     ASGWVLISRANGAALKFTPEGLLPALVTGVAFAFVTIFLYLTFATGVNISLAIPTIRI
                     AGILLASALGVLLLREPLTWRYVAGVALALAGIYLIATR"
     gene            21561..23045
                     /locus_tag="AC162G_11955"
     CDS             21561..23045
                     /locus_tag="AC162G_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076579.1"
                     /translation="MSTVLTPAEERGLSGLSLDSQVRKVFYSIPPETIRAMGERMVEE
                     AFRRSLIYERDGKPEAIRVLLRPIGIMPDQLAYLHYVSLTIVNALKRLPDLYIQDFAV
                     RQVVPLTPSEEKWVWDCYGPSHRDNSPVFGRLDAVVDFTSPMWKESLRFVEPNLCGVG
                     GLHLIPTCEQMLADVVLPVIQEYAPQIQLEIGQDLRELFIEEVLDHLDTIGRSGRNIC
                     FVEPKYAGDGPNEQAALAEYYHERHGLTVLHADPSELYMKKGDVWYEDSPVDVAYRDY
                     EVRDIVDLESEGTDVRPMRTLFKENRMVSSLAGDFDHKSCWEVLSDPQFTNKYFNAEE
                     RLVFRRHILWTRVLSDRRTALPDGEVVSLLEHTRKYQDELVVKPNRSYGGDRVLIGPS
                     VSQSEWEAAIEEAVTGDEPWVVQRLAPIAVNEFPVIAEDGAVQMEPFYTVLGFAPTRY
                     GLAVLGRASQKQVVNVAQRGGMCGVLIGKPSGRLIGPGAPVRMS"
     gene            23115..24542
                     /locus_tag="AC162G_11960"
     CDS             23115..24542
                     /locus_tag="AC162G_11960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR5076580.1"
                     /translation="MSRVPFNGRRPGMLGRAEIEDRLKYAVYLSEADETEAVIEASDL
                     SLTRFARNAIHQNVAETDALLEVRAAFQRRVGAASTNDLSRMGIYRAVKRACALARHA
                     PENPNWPGLPEPQPLPEVQAFDEAVAGITPEGRARAVADICGDARALNVAASGAFSTG
                     WSEYAVMNSKGLFAYAPSTQVDLTFLVERPQESASAYANATGWRLSQIDFQAVKRQAI
                     EHALADRQPRPVPPGEYPVVLEPYAVANLLDALSEAGMGALAVQEERSWMNGRIGRRC
                     LSPKVSIVDDAFDLNGIPQAFDVEGMPKRRVPIVVDGVPTSPVYDRLTAARERERAST
                     GHAQPFDDDWDGPLPENLHLAPGDVTVQEMIRAIHRGLYITRFWYVNLTTTHECGVTG
                     TTRDGVWWIERGELAYPVTDMRFDQELVPALEHVRGVGNDVRTLSGWAGGVLRVPALA
                     LESFRFIDVGDDRYTARKYDEAGDD"
BASE COUNT         4383 a         7544 c         8073 g         4635 t
ORIGIN      
        1 tttgcgtagg tgaacgcatc ggcggtcaaa tcgggttcag ccgagccgat ggcatctgga
       61 taccattgat tatccgcgcc gccaggccct gcgatcgaag cggccaggcc ggcgaatacg
      121 cccacgccgc tcaccgtgcg tgagccggag tcgtcttcga ctgcgatcgc cttcaggcga
      181 tcgtggtaat agctcgacca attggtcgcc agcccgccgt cccagaaacc cacatcctgc
      241 ccgctgagga gaagagagcc gccggccgac agatagctgc tgatcgcgtc gcccgccccg
      301 ataaacccgg gcgcatcctg cggcgcagac cagatgacgg cgtcgtacgc cgccagcgtg
      361 ctccactggg gcacgtcggc gggcaggcgc ttcacgcggt gctcggcgta ggggtagccc
      421 agtgcctcga gcgcagcccg gtagtaccca atctggctgc catagtacca tggaccggaa
      481 tcgaccagca ggatggtcgg cgcgggcggc agcgagaaat cacgcgtcac ggccgcgcca
      541 ggcgtaaccg tcaccgtcgc agtaagggcg cggtgacccc aggccacggc gcgcagctcg
      601 aacgttccac cgggcagcgt cgcgctgtac acgccgcgcg cagcggccga ggcggtgacc
      661 ggcgtgccgg cgacgacgat cgtcccgctg atcggttgcc ccgattcggc gtcggtgagc
      721 gagccgcgca cctcgccgga tggcacgggg tcgagcgaga agtcgagcaa tgtggcggcg
      781 ccggtgatga cgaagacagg cgcctgcgtc gccgtcacgt aaccgaacgc cgacgcgtcg
      841 accgtataga tcgaggcagc cagtccgcgc acgtattgcc cgtttgcgcc ggtgctcgcc
      901 gttccagagc cggcattcga gacggcggca acggtcgcgc cggcgatcgg agcgccggtg
      961 tcggatcgcg ccaccgtgcc gctgagcgtg cccatgcttg ccacagcttg cacggccgcg
     1021 taagcgtcga cgcgcccgta gccgctgtcg ttgttgggaa tcgtcgtgga cagcggcaca
     1081 gcagcgccgg tcaggacgaa cgccgtgccg gtgatcgtca gcgacgggtt ggccgcaagc
     1141 atgagcgcgg ccgttcccgc agcgtgcggc gcggctaccg acgtgccgct gtaacggtcg
     1201 aagatgccgc ctggcacgct cgatcgcacg ttgacgccgg gcgcggctac gtgcgggcgg
     1261 acttcgcccc acggcgacgg gccgcggctc gagaaaaccg cgacctcatc ttcgtcgtcg
     1321 gtcgcgccga cggcgaacgc ctcaggcagc gacgccggcg atccgatgcc gccggcgtcc
     1381 gggccgttgt tgccggcgct gaacacggcg aagatgcctg cttggcgcag cgcgtgcagg
     1441 tcactttgaa aagccgtgct ggcggagacg tcgctgcccc acgagttgtt cagcacgtcc
     1501 ggcgcgaggt tcgggtcgcc gtccggcgcg agtatccact cgaagccggc gtggatccat
     1561 gagtcgtagc cgaagccggc gctgttcaag acgcgggccg cgatccagcg cgcacccggc
     1621 gcgacgccga taccgtccgc cccgacgatc gttcccatgg tgtgcgtgcc gtggccgtgc
     1681 ggatcgacgg ggtacacggt ggcgtcatcg gtggcgtcga gccagttgcc ggcgtggccg
     1741 gccagcccct tgccatagcc gcggtatgcc gttatcagcg caggatgctg ccaatccacg
     1801 cccgtatcca tgttggcgac gacgatgccg gtgccggtga cgttgagcgc ggcccagacc
     1861 tcgtcggcgc ggatcttgcg gaggccccat tcgagagtta gagttagaga gttagagtta
     1921 gaggtagagg ttggaggtgg aaggctggat ggcggggcgg ttacccactg gcgaaaacgg
     1981 tcttcgcgaa tcaggccgac gccgtcgcgc gcggcgagcg cgaggatgtc cccggcggcg
     2041 acatgcgagg ccaggccgtt gaaaacccag aacgaacgca cctggctggc gcggcccgcc
     2101 ttttccatca cggcgagctg ggcgcgcacg cctcgctgcg agtgggctgc agtcgtccgc
     2161 agcgcatgga caactgcctc ggcctggcta atcccagcgg ccctcaatgg cgcactgtcg
     2221 agatcggcct gctgccgcat ctcgacgatg atcggaatct gctcgccagg cgcggcagcc
     2281 agcattcgct tgagcagcgg tggatcgatt ttgccgggcg caggcggacc atcgatcggc
     2341 gtgaggttac tcggcgcgga ttgaccgcgc ggatactgcc ccgtgccgtg atcttggcgc
     2401 tctgcgcgtc caggcaacgg ggaaccgctg gacacgcccg cgtcgtcggc gaatgcaaac
     2461 gacagtgcca gcacgagcgc gagcgcggcc agcagtgcgg tgagtgcgag ttgaagagcg
     2521 ggacggtaat gagtagaagg gttcatgctc aggcctgtcc atcaaatgcg tcacaaaagc
     2581 atacaccgaa cttgtgatat acgcgaatca ccccctccca gatcgcagtc gaataacaat
     2641 accggcgccg gtgtttgaca cacgcactac ggcagtctat acttggcgcg ggcataaact
     2701 gcgctttttc acccacgaat ggacacgaag tctcacaaag atttcgtgtc gcttcgtgtg
     2761 ccttcgtgga ttcccaataa cgcaagttgt gaccgccggc agtctacaag acacgcggcg
     2821 ttcatctcgc ctgaaggacg actgggatgc gtttgatcct acggtttgga atcctggcgt
     2881 gttgctttgc tgcactctcc gcctgtggcc ctccgcctcc tcccgaactg cctcctgacg
     2941 agatcgtgca gcgtgcggcg aagggcatgg ccagccagaa cacgctgcgc ttcacgatcg
     3001 acatcgcggg cacgccggcg atgctcaatc cggcgctggg gttagcgctg cgctcggggg
     3061 agggcgtgtt cgcacggccc gatcggatgg gcgtgcacct gaagctcgtc ggcccggtcg
     3121 cgatcgaggc cgacatgatc gcgctcggcg acgagcagta catcaccaac ttcctgtcaa
     3181 aggagtggga gccgctgccc gccgagatcg gcttcaatcc ggcggtgatg tttcaccctg
     3241 aattcgggct ggagaagacg ctggaaggcg gactggacga tgcagagctg gtcggcgtcg
     3301 agtctatcga cggcgcgcag gcatactacg tgaagggccg cattgagggc tcgcgcctgt
     3361 ggcggatgag cggcggcctg atcggctcca gcacagtgga tgtagaggtg tgggtcgatc
     3421 cgcagacctt cgccgtgcgc aagatcgtgc tggttgatcc gaccatcgat cccggcaacc
     3481 cgtcgatctg gacaatgacc ttctcgaagt tcggcgagcc ggtgacgatc gaagcgccgg
     3541 acgtctaatc tccaacttcc aacttccaac ctctaactcc aatctctaac tctaactcct
     3601 gacttccgtg ccctactcct cccgcctgcc acgccccaac gcgatcatcg ctctcgtctc
     3661 cgtagcgatc ttcgtcgggg cgatggacct caccgtcgca tcggcgttct tgccgcaagt
     3721 cgtcgtcgat ttcgagctga ccgtggatca atcggcgctg gccggctggg tcgtgacgat
     3781 gtacctggcc gcctacgcgg tgagcatgac gttcgccggc cgcctgagcg acctgtacgg
     3841 gcgccgtgcc gcgtaccttg cctgcctggc gatcttcgtt gccggctcgg tcatggtcgc
     3901 cgtgacgcag cgcgcgccgt tcgcgcagtc gctggagtgg ctcgtgatga gccgcgtggt
     3961 gcaggcgctg ggcgcgggcg cgatggtgcc cgtgtccatg gcgctcgccg gcgatctgta
     4021 tcccccggaa aagcgtgcca cgccgctcgg cgcgatcgcc gccgtcgaca cggctggctg
     4081 ggtagtgggg catctgtacg gcgggatcat ggttcgcctg ttcggcgact ggcgcatcat
     4141 cttctggctt aacgtcccga tcggcgtcct cgcctttatc ctgtgctggc gcgcgctgcg
     4201 cgatgcgccg caatcgaaag ggcagggagg gctggactgg atcggcgtcc tgctcattgg
     4261 cggcgcactc gtggcattca acataagcct gggcagtgga gccgagtccg gcgcagtttc
     4321 gccgttcgtc gagcagacgg cgccggcgga tcagacgccg ctcttgctcg ccgccgctgt
     4381 gctgtcttgc gcgtttatcg tgtgggaact gcgcgcgcgc gacccgctgc tcgacatgcg
     4441 gctgttccgc aaccggacct tcaccgccgc ctcgctcacg aaccttctgg tcggcttcgt
     4501 cctgatcgtc gcgctgggat tggttccact gtttgtcaac gtagtcgtcg cgtccagcca
     4561 gcccggcctg acgctggagc agatcctgag cgacggcgcg tggtacaccg gctgggtgct
     4621 atccggcctg acgctgacga tggcgatcgt gtcggcgttc gccggccgta tcgtcgagcg
     4681 acgcggctat cgtctgcctg cgtcggtcgg gttggccctc ggcgcgattg ggttctggat
     4741 agccagccga tggacgtccg gcaccacgta cttcgaaatg ttgccgggcc tgatggtagc
     4801 cggcgcaggt ttcgggctgg tgctggcgcc cgtggcgacg gcggtcatca acgtcgcgcc
     4861 ggcgcaggaa cgcggcgtcg cctccgcgct ggtcatcatc ctgcggctgg tgggcatgag
     4921 cctgggcgga tcgaccgctc tggcgtggtg gacgcagcgt gtccagacat tgacggcgaa
     4981 cctgtcggca ggctcttcgt tgttcagcgg ggacgccttc gaggttttcc gcacggcgac
     5041 ggcgcaggcc gtgggcgagt cgttcatcct tttcgcagtc ctcccatgtc ttgccgggct
     5101 gctgccggct tcgcaattgc gcagcgagga cgagctccct agtggctctt ttccaaaggc
     5161 atgagtagcc atgaagtgct ggtgctactt ctcctcgatt tgtttgagga ctcttgtttg
     5221 tatacgcgct ggaagccccg ctgcttgcat ggcttgccgt agtaactttg tgttacagtc
     5281 gtgagtccga atcggtctcc cattcaactg tactgacctc aacttgaaca ccgttgatgt
     5341 taaatgcggt gtaatactgc cgtgcatccg acatccaagt cggcgggcta atgcagggaa
     5401 agtctgccag tgcgcgagcg aagatatcca cgctttcacg tttctctacg aggatgtcaa
     5461 catcgccggc acgcatagat acgccttgtg cgactgcggc tgccgtaccg acgaaacaga
     5521 accgttggcc tttgagatac ttggcgacga tgtgcgccca atggcacgca gatcagccgc
     5581 gcgagccgcg agagcggtcg gaactcgcag agccgcttgc ggcgaagcgt cggggggtta
     5641 tattctttcg actctaccgt cttgcaggcg cacttccccc gtctccaaca actgcttcag
     5701 tttagcgaga ttctccgcaa ctgcggcttt gacccggccc gccgcgaatg aaccgaataa
     5761 gccaatcagt ccacccttca actcccattc atcggtaagt cttgtgccgc cgcccgcagg
     5821 ctcaaacgta aaccgcatct gcaacgccgg ggctggcggc agcgtctcga caacgacttt
     5881 acggttaggc tcaaactcaa cgatcttgta ccgttgagta tccgtcttgc gtacttgcct
     5941 gaagactgtt ccgacaccga tggggctggt ggtgaccttg tttacttcaa ctacatagta
     6001 attccatttt ggcatattct caaaatcagc cacgaatcga aaaacctcat tgatcgggcg
     6061 accaatgatt aagttgttgc taaagcgtgc cattgaagtg ctcccttgcc cgaaccgggc
     6121 gctacactgc cccgaccatc aaaacacatg cttccaccgt aaggtacatt cataaaggtt
     6181 cctcatgtca ggctctcgaa cgagcggcgg gctacgccag ccaagcctga tcaagcggtc
     6241 tggcatagcc ttctttagac cggttggcca ttgtagcctg cgaattcgtt cgcgggcgtg
     6301 cccggcgcac cgcccaacgt cgtcggcctg gggcggtgtg tcctagtggc gcgggtgcga
     6361 ttctagcaca ttgacacata tagggttgca tattatcatt taaaatactt taatcgctag
     6421 aagaaggagg atgctaccat gagtgaaaga gaattcatgc gagtcagcga agcggagatc
     6481 gccgttcact ggcaggaaga ggaatattac cacccctcgg cccaatttat tgctcaggcc
     6541 aatatggctg acccagaagt ctatgagcgg ttcagcctgg agaatttccc cgaatgcttc
     6601 aaggagtatg ccgatctgct gacctggtat cagtattggc atacgacgct ggatacgagt
     6661 gatgcgcctt tttggaagtg gtttgttggc ggcaagatca atgcgtgcta caactgcgtc
     6721 gatagacact tgccgaagta taaaaataag gcggcgatca tttgggttcc tgaaccggag
     6781 gatgagcctc atgtggcgct cacttatcgg gagctttacg tccgagtgaa tgaggtggca
     6841 gcggttctga gagactttgc cggactgaag gccggcgata gagtcacgct ccatttgccg
     6901 atggtgccgg aactcccgat cacgatgctg gcttgtgcca gactgggcgt cattcactcc
     6961 gaggtatttg gaggatttag cggcaaggcg tgtggtgaca ggatagcgga ctccggaagt
     7021 catgtcctga tcactatgga cggctactac cggtccggcc aactcggcga ccataaggaa
     7081 aaggctgacg tagccctcaa ggcagcggaa gaacagggcc aaaccgtcga taaggtcttg
     7141 gtctggagac gccaccgtgg gaagtatgtc tcccaaacgc ctatgagagc aggccgtgat
     7201 tactttatgg acgatctgat tagggattat ggcggcaaaa gagtcgatcc cgttcccatg
     7261 ccggccgaag cgcctctctt cctgatgtat acgagtggga cgacgggccg gcctaaagga
     7321 tgccagcata gcaccggcgg ctatctggcc tatgttgccg gtacctcgaa gtatattcaa
     7381 gacattcatc cggaggatgt ctactggtgt atggcggata tcgggtggat caccgggcat
     7441 tcatatatcg tctatggccc gctcgcgctg gctgcgacga gcgtgatcta cgaaggtgtt
     7501 cccacttacc cggatgccgg gcgtccctgg cgaatcgccg agcaactgga cgtgaatatt
     7561 ttccacacgg cgccgacggc gatacggatg ctcagaaaag cggggccgga cgagccgacg
     7621 aaataccatt atcagtttaa gcatatgacg acggttggag aaccgataga gccggaagtg
     7681 tggaagtggt attaccacgt cgtcggcaaa ggcaaggcgg tcattgtcga cacgtggtgg
     7741 caaacggaaa acggaggatt tctctgcagc acgatgccgg cgctgcatgc gatgaagcct
     7801 ggaagcgctg gtccgggggt gcccggtatc catccggtca tctacgatga gaatgggaat
     7861 gagatcgaac cgggctcgga taaggccgga aacatatgca tcaggaatcc gtggccaggg
     7921 gaattccaga cgatctgggg cgaccgggag cgatacgtcg cgcaatatta tgcgaagtat
     7981 tgcaagaaca agaacagcaa agactggcgt gactggcctt actttaccag cgatggggcc
     8041 atgcaggcta aagatggata cgttcggata ttaggcaggg ttgacgacgt catcaatgtg
     8101 gcaggccatc gactgggcac aaaggagtta gagtccgctt gtctgaccgt cgacaaagtg
     8161 gcagaagcgg ctgttgtgcc cgccgtggat gaggtcaagg gccgtgtgcc tgatatttac
     8221 gtgtcgctta agcccggcta tccatccagc aaagagaccg gatatgaagt gatccgggcg
     8281 ctggagatga tcattggcaa aatagccagg ccgaggcacg tctatatcgt gccggacatg
     8341 ccgaagactc gctccgggaa gatcatgcgc cgtgtcctgg ccgctatctc gaacaatatg
     8401 gacgtcggtg acgtaacgac gttagccaac ccagaagtgg tcgaggaaat ccgcagaatg
     8461 gttcaaggag agacggtgat cgttacgcaa gaggtgccgg aagatatcag gcaatttggt
     8521 gaggaacgtt gataggcatc gtcgcgaata gcaagccccg ccgagcaggc ggggcttgtt
     8581 tgtgctttgg ccctttttca gacttcggaa gtctcattaa agacttccga agtctcggtc
     8641 gtgggcaacc tacggcacgg gcgtgcacga cacgctttcg ttgggcgggc cggacgtgac
     8701 gcaggtgaat tcctgctcca gtaagacctc accagacgcc gcatccgtca ccgtcatgcg
     8761 cgcgtagtcc cagtaaacaa agaggtcggt cactttccag ttgttgttcg cgcagccggc
     8821 ttctcttgcg ctcggcatcg gaggcgcgtc ggtgaccacg gtgaacgggg cagagccgtt
     8881 ttcgatcaga ctggccgaga cctcctgaat gccggagacc tccacctgaa tgggattctg
     8941 gcccggcgct tcgttgccgc ccttattggc acacgtcgcc gtgccaatac ccgatccgac
     9001 gagttcgaca atggcgtcgc ggtttccata cccggctaat acgcctgacg cagtcaggga
     9061 ctctcccagt gagaacgaga ctgcgccgac gaagtgtcct ctgccggcga atacaggcgc
     9121 ggtcatcagg ctgagtgcga cgagggttgt caggatagaa aacaggcgct tgcgcatgat
     9181 tcactccctt tctcttcggt gcgtgggcca tgccccacgg ctgattcaat gcctcgagca
     9241 caggtcgtcg agatcacctc attccatccg ctgacgacgg acattatagg cgatgaatcg
     9301 ggagttcgcc gtgagaaaac cgttagaatt tgaatcaagt ttgaggtttg ttactggtta
     9361 accacgctgt tctctccatt gtgtcgccgg cgagacagag ttatacttgt caatacaggc
     9421 gattctggtg attatctaca agtcggtttg tccgctgttg cactgcggat gttgggcgac
     9481 agtgcaactg tcgccccgac aggtgtgcaa tcaccaaagg cgattctcac gccggatttc
     9541 ggctgcccag agacgatgtc gtgaccgaac gcgaacagat ggagcaggcg atacgcgcgc
     9601 ttgaagccca gcgcgccatg ctcggcgatg ccgtggtcga tacggccctc gcgccactgc
     9661 gcgaaaagct cgccgcgctg gaggcgcagg cagaggcgcg ggagcggcag cagcgcaagc
     9721 aggttacggt gttgtttgcc gatgtgtctg gcttcaccgc gatgtccgag aaggtcgatg
     9781 cggaagaaat gagcgccacg atgaacgcgc tctggtcacg gctcgacaaa gccatcgtcg
     9841 accacggcgg catgatcgac aagcacatcg gcgacgcggt gatggccctg ttcggcgcgc
     9901 caacggcccg ggaggacgat ccagagcgcg ccatccgcgc cgcgttggcg atgcagcggg
     9961 aactggccga gtttcgagcg catcttgaca gcatgcttgt cgcgcgaccc ggcgccgggg
    10021 tctcgctgcc ggagatcgcc atgcgcatcg ggatcaacac cggtccggtg ctcctcggcc
    10081 aggtcggcac cacgagcgag tacacggcca tgggcgacgc ggtcaacgtc gccaaccgcc
    10141 tggagcacgc tgcgccggtg agcggcatcc tcatctcaca cgacacctac cggcacgtgc
    10201 gcggcgtatt cgacgtgctg tccctggagc cgatcagcgt caagggtaaa tcggagccga
    10261 tccaggtata cgtcgtgcgt ggggcgaagc cgcgcgcgtt tcgcgtcccc acccggggcg
    10321 tggaaggtat cgaaacgcgc accatcggcc gcgaggctga gcttcagttg ctccggtcgg
    10381 cgatggagac ggcgcgacac agtcgccgga cgcacctggt cagtgtcgtc gccgaggctg
    10441 gggtcggcaa gtcgcgcctg ctgtacgagt tcaacaactg gctcgagctt ctgccggagc
    10501 gcatcctgct attcaagggg cgcgcttccc aggagatgat caatcttcct tactcgctca
    10561 tccgcgacgt gctggccttc cggtttgaga tacaggacag cgatcgcgcg tcgaccgcgc
    10621 gcgacaagat cgagcgcggc atcgccgagt tcctcggccc ggtggccgtg gagaaggcac
    10681 acttcatcgg ccacctggtc ggcattgact tctcgcacag cccgtacctg cagggcatcc
    10741 tgggcgacgc gcggcagatc cgcgaccgcg ctttccacta catagggcaa ctgttcgagg
    10801 cggtgacgcg cgagcatgcg gcagcgatcc tcctggagga catccactgg gccgacgatg
    10861 gatcgctgaa tctgatcgaa tacctggccc gcgagcggcc cgaccttcct ctcctgatca
    10921 tcggcctggc ccggcccacg ctgttcgagc ggcagtcggc gtgggacgcc gagctgccat
    10981 cgcacattcg cctcgatgtc cgtccgctca ccgagccaga ctgccgccgt ctggtcgcgg
    11041 agattctgcg caaggtaccc atggtgccgg ctgagttgga cgatctcatc gtgagccggg
    11101 ccgaaggcag cccgtattac gtcgaagagc tgatcaagat gctcatcgac gacggcgtga
    11161 tcgtcgcccg cgaagatcag tggcgagtgg agctggagcg cctggccgcc gtgcgcgtcc
    11221 ctgcgacgct gaccggcgtg ctccaatctc gccttgacag cctgcctcca ccggagcgcg
    11281 aggtcctgca gcaggcgtcg gtggtcgggc gcgtgttttg ggaggaggtc gtcgcgcggc
    11341 tgcatcatcc ggatacgccg cgcattgaat taccggcgac ggcgagcgag agattggcgg
    11401 cactgagccg gaaggagctg gtttttcacc acgacccatc ggccttcgcc ggcatgcacg
    11461 agtacatctt caagcatgcc ctgctgcatg atgtgaccta cgagagcgtg ctgatgcggc
    11521 tgcgccgggc gtatcacgcc caggtcgcgg caggcctgat cgagctaagt ggtgaacggg
    11581 tgggtgagtt tgccggtcgt atcggcgagc acttcgagcg ggcaggcgag tggctccagg
    11641 cggcagaatg gtatggtcgg gcggggaagc aagcgcgcga cacctacgcg ccggaagcgg
    11701 ccatcagcta ctaccggaag gcgctcgaat tcgcgaaaga attcccggcg acggcgtcgc
    11761 gcttcgatct gtatgcgggc ctcggcgaag cgctcgtcga tcaggcgcgt tatgcggaag
    11821 cgatggacac attcacggcg atgcgcgagg cggcagacgc cgccggtgac cggttggctg
    11881 aggcacgcgc ctggtatgga ttggcaacgg cacaggtcaa ccacggtgat catcatgcgg
    11941 cggtggtgag tgcagagcgt gcggaggctt tggcgcaggc ggcggatgca cgtctggaat
    12001 tggctaaggc gctcgagatc aagggcggcg gcctgtatcg cctgggcgat gccgaagctg
    12061 cccgggcggc cgtcgagcag gccatcgtca tcgcaacgga gcttgacgcc cggcgcccga
    12121 tggcgagcag cctgaacctg ttgggcctgg tgcataacat gctggggcgt tacgagctgg
    12181 ccgagcgtca cttcgagaag gcgattgcca tctgccggga gctggaggat cgccggcggg
    12241 taatgtccct ggtcaacaat ttgggcgtca ttgccgaagc gcgcggcgac tatgcggcgg
    12301 ctctcgagcg ttaccgcaat gcgctgcaga tcgctcgcga catcgggcat cgcgacggcg
    12361 agtttgtctt cctcaacaac gtcggagcgg cgcgcgtcgg actcggagag tacgtctccg
    12421 ccgaagccga tctgcggcac gtgatccaga tggccggccg cgctggaccg gggatgctgt
    12481 ccaatgcgca tggctttctg gccgaagcct gcctgggtca agggaaggtt gcggaggcgc
    12541 tcgaggcggc gcgactggcg ctggggctgg ccctggagag cgacgagcag gaaggcattg
    12601 gggcggcgtg gcgtacgctg ggccaggtcg tggcgcagtg gccggaaccg gtcgcgatcg
    12661 gcgccggtcc ggatccgtca cggcgcgtcg acgcagtggc ctgcttcgcc gagagtcttc
    12721 gcgtgtgcac cgaaacgggc atggaaggcg agcgggcgcg cacactgaag gcatgggcga
    12781 cgcacgaact gcagcgcggc gaccgcgcgc gcggcgccga gatgtggcaa gaagcgcacg
    12841 ccacttttgc gcggctggga gccgatcggg aagccgagcg gatgagccgg atgccgtgaa
    12901 cggcttacgc cgtcagatca cgtttccagg atgagggcat gcgcctgcgg tcgtactcga
    12961 tatctttcgc gccgaggaat tccgccagct cctcgatcgc tttcgcgaca gcgcgtgaga
    13021 cggttcgact attgggtgcg tcgggttcgg cgtgcaccgc gttgatcgcc agcacgctcc
    13081 gcgtgcgatc catcttcgga tcgatgcgcc ccacgaagcg gtcgccgtgc aagatcggca
    13141 ggacgtagta gccgtgcttg cgcttcggcg ggggcacgta aatctccacg gtatagtcga
    13201 agccgaatat ctgcttggtg cgtgcgcggt cgcagatcaa gttgtcgaac ggcgagagca
    13261 gcgtcgtgcg cggctcccaa tgttcaatct ctaatctctc cagaagcggc aagtcctctg
    13321 cgtgcatgta ccactctccc tgccacacct gcccgccgtc tcgaatctcc actctctcga
    13381 ttcgagcgtc cgattgaagt tcggacagca cgctcttcaa gtcagggtag cggcctcgaa
    13441 tgtagtgcaa cccgatgtgc cggggcgtcg ccacgccgag cgcgcgtagc gacttctgcg
    13501 cggcgcggcg caccacctcg cgctcggcca gcttctcgcg tggcgtccat tttggcagac
    13561 agcgctcgct cacgtcccac agcttctgga gacccgatcg gccggcaacc atgatctgcc
    13621 ccttcgtcca caggtagtcg agcatctcgg tcacgtttcg gcctgaagtc cagccggtgg
    13681 aataccagtc ggacctggcc tcgtcttcga agtagcgcgc gggcaggggg ccgttgcgat
    13741 gcagctcgcc gagaatgtgc cggcgcaagc cggccttgtc tttcagccac tggcgaagct
    13801 tcttcgctct cggcgtcttg ggtgtgccgg ccgcgtaccg ccgcatcaag agattgtgga
    13861 tgggatagtc ctcggtgagc acgatcgacg cgacgtgcgc ccagtactcg aacaggctgc
    13921 gatctttcca gagcagcgtg tcgaggtcgt ccaggtcata tgggccgagg cgactccaga
    13981 gcacgagctg atggctgcgt gcgacggcgc tgatcggatc gagctgcagg cagcccaggt
    14041 cgcgcacgac ttccaggatg cccggcggat cgacggccgg gcgcgggtcg gccaatcgct
    14101 gcttggtgat ggcgagacgg cgggcaaggg taggggataa ggtgtgcacg ctttcctcaa
    14161 aagacctgcc aggtctcgca gacctggcag gtctgtcaac ggtgattata taactacaac
    14221 gtcggcgtgc catcccccag ttcggcagcc ggcctcctgc tgcgcgcgac gcgcgcgtgt
    14281 ggacgcgacg cgatcgccca tacaccgagt ggcaccacgc tgctggcggc gagcagcagc
    14341 gagaaggcgc gcggatcgag cgcgaactga tcgagcagcc agcccgtcag ccacaccgat
    14401 gccgacatcg ctagcgtgaa caggccgaaa tcgagtgcgt ctctggccga cgggaatccg
    14461 ggtgcacttt gccaaccgtg gcgtcgtggt aaaatgcata cgccatgcgg ttgaatcaac
    14521 tcatagcggc gctcccgtcg ttggaatcgc gccccgattc ggatccggag atcacgctcg
    14581 tcaccgacga ctcgcgtcga gtcgcgccgg gtgcgctgtt cgtcgcgtat cgcggcgtgt
    14641 cggtagacgg gcaccgcttc gtccccgacg ccatcgcgcg cggcgcggtg gccgttgtgg
    14701 gtgagcgcgg agacctgaca ggtttccaaa acctgtcagg tcttgacgtt ctctatcttc
    14761 acgtgcgcag cggccgcgag gcgctcgcct atttgcacgc ggcgtggaac gatttccctg
    14821 cgcgcaggtt gatcatgatc ggcgtcaccg gcaccgacgg caagacgacg acgaccaatc
    14881 tgatttactc tatcctgaag gtcgccggcg tcagggcggg catgatcagc acggtcaacg
    14941 cggtgatcgg cgatgagatg ctcgacacgg gcctgcacgt caccacgccc gacgccgacg
    15001 aagtgcaggg ctacctgcgc aggatggtcg acgccgggct gacgcactgc gtgctcgaag
    15061 tcacctcgca cggactggag catcaccgcg tcgacgcctg cgagttcgac gtcgccgtcg
    15121 tcaccaacat cacgcacgag catctcgaca tacacggctc agtggaggga tacctcgccg
    15181 ccaaagcgcg gctattcgaa tcgctggcgg aggcatacga caagggcgtt cccaagacgg
    15241 cggtgctcaa cgtcgacgat gcttcgtatg gctatctgcg cccgcgcgtt cggggacgtg
    15301 tgctggcata ccggattgtt cgccacggag acacggaggt cacggagact catggagaaa
    15361 acgccatgtc tgcgtggcga gatcgcgtga ttcaggccac ccacgtcgtt tatgaaccgg
    15421 acgcgattcg gttcgtcgct cagggcgacg actttagcgt tccgatcgaa acgtcgctcg
    15481 tcggcgagta caacgtgtcg aactgcctgg cggcgatcgc ggcgacggcc agcgcgctcg
    15541 gcgtcccgcc cgaggtcacc gcgcgcggca ttgccgggct gaagagcgtg cctggccgca
    15601 tggagcgcgt cgacgagggt caggctttca tcgccgtcat tgatttcgcg cacacgccga
    15661 acgcattgga caaggccatc gccgccgcac gcacgatgac gcagggccag gtgatcgtcg
    15721 cgttcggatc ggctggcctg cgcgatcgtg agaagcgccg cctgatgggc gaggtggccg
    15781 gccggtatgc ggacaaagtt gtgatcaccg ccgaggaccc gcgcaccgag tcgctcgatg
    15841 ccatcatgga tgccaccgcg cgcgccgtcg ccgcgcaggg gcgcgccgaa ggcgtcgatt
    15901 tttggcgcgt gccggatcgc ggcgaggcga tcgcctttgc ctgctcgctc gcccgggcgg
    15961 gcgacgtcgt gatagcctgc ggcaagggcc acgagcagag catgtgcttc gacgacatcg
    16021 aatatccttg ggacgatcgc gaggcgatgc gagcggcgct gcgtggtgag ccactacgga
    16081 ctttgccgac tgctcagcct cgcgtgggat gagcgcgccc acgccaccta acgtcggtgt
    16141 attaccatat gctcgatctc gccgtcgtta tcgtgtcata caccgtgcgc gatctgctgc
    16201 gcgactgctt gcgatcggcg ctggcgaacg aaggcctggc gttctcgatc tgcgtggtgg
    16261 acaacgactc gcccgacggc agcgccgata tggtcgagcg tgagttcccc caaatccatc
    16321 tcgttcgcaa cagcaatagc ggttacgccg ctggcaacaa cctggggctg agatatttcg
    16381 gtttcggcga aacacccaat ccaacttcca acctccaact tccaacttcc aatctccaat
    16441 ctccaatctc caatccccag gttccgacag ccacgtcgcc gcgttacgct cttctgctga
    16501 accccgacac cctcgtcccg cccgacgcct tcgcgcgcgt cgtggcctac atggacgcgc
    16561 atcccgacat cggcgtgctc ggcgtgaagc tggttcgcgc cgacggctcg ctggatctcg
    16621 cctgccgccg ctcgtttccc acgccggaag tgtcgtttta tcggctcgcg ggcttgagca
    16681 agctgttccc gcgcagcccg cgcttcgggc gctacaatat gacgttcctg gacccggacc
    16741 agcatgccga ggtggactcg gtcgtcggcg cgttcatgct cgtgcgtggc gaggccatcg
    16801 cgcaggccgg cctgctcgac gagacgttct ggatgtacgg cgaggacctc gactgggcgt
    16861 accgcattaa gcagcgcggc tggaaagtgt tgtactaccc ggaagtgacc gtgctgcatc
    16921 tcaagcggtc gtcgagcgcg ggcagcgcgc gggcgcggta cgagttccag cgcgcgatgt
    16981 gggtgttcta ctgcaaacac tatcgcgccg cgacgccgcg ctggctcgat gcgctcgtgc
    17041 gcctgggcct cggcctgcgc ggcggccggc ggctgctcgc cgagatgtcc cgcgacggag
    17101 gcaggcaatg agcaagggca aggtgcgcgc tttcttcggg attactttgg tgctcagcga
    17161 tatactgatg gcggccatgg ccttcgtgtt ttcctacctc cttcgactgt cgctggcgct
    17221 gcccaacgcg gcggtcagcg tcgcgccgat caatgagtac ctgggactga tggtggtctt
    17281 tgtcgggtcg atggtcatcg tgttcttctt cgcgcgcctg tatcaccagg ggcgggccat
    17341 ctcgcgcgtc gacgaactgt acaccgtttt cggcgcggtg tcgatcagct ccatgatgtc
    17401 ggtggcgctg tctgtgctgc tgttcaagaa cagcgcgttc gagctggact ttccgcgcgc
    17461 gatggtgatc tacgcctggc tcctgacgat cgtcctggtg acgctcggcc ggctgctcca
    17521 tcaccgggtg cagggacagt tgcaagcgcg cggcgtgggg cgcgaccggg tgctgatcgt
    17581 cggcgcgagc gagaccggca acatgatcct gcagaagatc aagtggtcgc ctcaactggg
    17641 ctaccaggtc gtcgggctgg tcacgcgcaa cccggacttc gcgctgagcg aagtgttcgg
    17701 cacgacggtc gtgggcgccg tcgacgatct gccgcgtctg attcaggagc atcaggtgga
    17761 cgaagtgatc atcgcgctgc cggaggccac gcatacggag gtcctcgacc tggtcgccaa
    17821 gtgcgaccac ggaaggatca acgtcaaagt gttccccgac gtctttcaga tcattgccag
    17881 ccaggtgtcg atcgatgacc tgggcgggct gcccttgctg tcgatgcgcg acgtgcggct
    17941 gcgcggctgg cacgtcacgc tcaagcgcgg attcgatttc gtcgtttcgg cgatcgtgct
    18001 cgtgctcctg tctccgctgc tgctgttcgt ggcgctgctc gtcaagctgg agtcgcccgg
    18061 gtcggtcttc tacgcgcagg agcgcatggg acttgacgcc aggccgttca tgatgctgaa
    18121 gtttcgctcg atgcgccgcg acgcggaggc gaacggcccg ggctggacaa ccccggacga
    18181 tccgcgccgc acccgcctcg gcgcgatcct gcgccgcctc aacgtcgacg agctgcctca
    18241 attgatcaac gtggtggtcg gcgagatgag cctagtcggc ccgcgccccg agcggccggt
    18301 gtacgtcgag cagttcaaga agacgatccc gcgctacatg gagcggcaca aagaaaaggc
    18361 cggcatcacc ggctgggcgc aggtcaacgg gctgcgcggc gacacgtcgg tcgccgagcg
    18421 caccaagtac gacctgtggt acatcgagaa ctggtcgttc ctgctggatc tcaagatatt
    18481 gattcgcacc ttcttcaggg tgatcaacga ccgaaacgca tactgaccgc gcccgttcct
    18541 tatcccgctt cctgagaatc aaccacggag atcacgaaga tcacgaaggt tcccggtttg
    18601 acaaggcggg gcgatcttcg tatactgcgc ccaaccgggc aatattcggt gatcaggaga
    18661 ctctatcatg caggatgctc cgatcctcaa gcttacgatg cggcaaggcc cacgtcccga
    18721 tcagaccttc gagctgcaca aggatgtcta cacgctgggc cgcgaggccg gcaacgacat
    18781 catcatcaac gatccgcaga tctcgcgcca tcacgcgcgc ctgacccttc agggcggctc
    18841 gtacgttttg gaagacctgg gcagcaccaa cggctcgttc gtcaacggcc gccgcatcac
    18901 ggggcccacg gccttgtcgc cgggcgacat ggtcggattc ggcgacaccg tggttttgtc
    18961 cgtgtccggc gcgatggatg cggccgccac gcaggtgggc cgcccgccgc aaaccgcccc
    19021 gtccatgcag gctgcgcgcc cttccgctcc aaccccgtcg cagcctccac ctgccactca
    19081 ggccggggtg ccgcaaaata cacagcggat gttggtgatc ggctgcgcgg cattaacgct
    19141 tgtcgtcatc atctgcatga tggcgctggt cgcctggtct ttcctcgact gcccgtcgtt
    19201 cgaggccttc tgggagagtc tgcccatcct gggcagtatc caggtcagtt gttagacaaa
    19261 taagaacccc ggtctctcca agagaccggg gttcttattc tgcgctccgc cagtacacgg
    19321 tttctccgcg ccgaaagctt tccacgcgcc tttgctcctc cagccactcc aggtggccga
    19381 tcacctccga cagcgcaagg aaagaatcga tggggccgag cctcccgaac atcggcccgc
    19441 tgacttcgta cgccgtcatc tccccattct cgagcaggct gacgatgcgc tgcgcacgcc
    19501 ggctgtggaa catcaaccgc tgccggacga gcttgtccac gtcggtgatc ggctcgccgt
    19561 ggccggtcca cgcgatcgat ggatgcatgt cggcggcgcg ctgcatctcg cgaacgtact
    19621 cgaccagccg gtgcggcttg ggcgcgccgg ggtgcggcgg cggctcgacg accgggttgc
    19681 tgctgatgtc gcgcagcaag tgatcgctgg agagcatgag gcgcgattcc ggctcgaaga
    19741 agcacaacat gccgcccgcg tggccgggca tgtgcaacac gcgccacgtc ttcccggcga
    19801 agtccacgcg gtcaccttcg cccagctcgc gatggatgcg cacggcttcg gtgaagcggc
    19861 cgacgcctct ccgggcgacg gccatgcgtt cctgctcgtc cggcgggacg ccggattctc
    19921 gcatgagctg gctgtaaaac gctagccagc gggcgcgctc ggtctcgtaa tcttccagcc
    19981 acatgcggtt gtaaggatgc gtccagactt cactcccgtc gccgtctcgg gcgatctccg
    20041 ccgccagccc gcaatggtcg gagtgcgcgt gcgtgacgag gatacgccgg atgtcgccgc
    20101 gcttgaggcc gagcgtatgc agcgcctcgc cgagcgcctg gcgcgccgcg tcgtcgcgcg
    20161 ggccggtgtc gatcagcgtg atcggctcgt ctgtggcgag gtacacgttg accggtccca
    20221 cgggaaacgg cgtcggcagt gtgatctgat agagatgatc gaggatgggt cgaggcatgg
    20281 gggtcagaga gattagagat tggagattgg ccacccatct ctaatctcca atctccaatc
    20341 ccattgggta aaggcactca tcaagcgacc ctctattcca ccttcggctt gcctttcacc
    20401 acgatcgtcc attcgccgcg ctggacggtc tcgccgcgct ggttcttgac gacgacgttg
    20461 aacgtgacga agccgccgct tgtgcccatc gcccgcgtct tcgtgacctc ggccgtcatg
    20521 tggacggtat cgccgatctt gatcggcgcg cggaacttcc agtcgagcga ggtgaaggcc
    20581 tcgaccgtgc gatcgaggaa gccgagctgc atcgccagtc ctgacgcgat cgacagccca
    20641 agcagcccgt gcgccacgcg ctcgccgaag atcgactgtt tggcgtaccc ggcgtcgacg
    20701 tgcatggggt tccagtcgcc ggacaggccg gcgaactgca tgatgtcggc ttcgctgatg
    20761 gtgcgtccgg cgctcgtgac tttcaggccg gtcgtcaggt cttcgaagta caagccgcgc
    20821 ggttggtagt cgctcatggg ttggctccga tgaaaggaat tggggattag agattggaga
    20881 ttggcccgct gcgattgtag cggggattgg ggtgatggtc aaatgcgacg tgcgacgcac
    20941 ttccaaagtg cgtggcacgt tgagtccgag tataattggc gcattgttgc gccgaggcaa
    21001 ccatcatgaa gggtcgggaa acaacccttg acgacgaaag gacgagccat ggactggtta
    21061 ccgttcgcgg tcggcgcggc ggtcgcgctg gcgacggccg atttctcggt gaagctggcc
    21121 gcaggtcaga tcggcagtca aatcggcgcg ctcatctaca gcctgacgac gctggtgatt
    21181 gcgagcggct gggtgctgat ttcccgcgct aacggcgcgg cgctcaagtt cacgccggaa
    21241 gggttgctcc ccgcactcgt gaccggcgtc gcgttcgcgt tcgttacgat ttttctatac
    21301 ctgacgtttg caaccggagt caacatttcc ctggcgatcc cgactatccg catcgccggc
    21361 atcctgctgg ccagtgcgct gggagtgctc ttactgcgcg agccgctgac gtggcgctac
    21421 gtcgccggcg tcgcgctcgc gctggccggg atttacctga tcgcgacgcg ctgacgacac
    21481 cgacgtgcga cgcacttcca accctctccg ctggctacga gggcgagagt gcgtcgcacg
    21541 tagtacacga ggagcttgcc atgagcaccg tgcttactcc cgccgaagaa cgtggcttga
    21601 gcggcctcag cctggacagc caggttcgca aggtcttcta ctcgattccg ccggagacaa
    21661 tcagagcgat gggcgagcgc atggtcgagg aggcgttccg gcgcagtctg atttacgagc
    21721 gcgacgggaa gcccgaggcg atccgcgtct tactacggcc gatcggcatc atgcccgatc
    21781 aactggcgta cctgcactac gtctccctga cgatcgtcaa cgcgctgaaa cgtctgcccg
    21841 atctgtacat tcaggacttc gccgtgcgcc aggtcgtgcc gctgacgccg tcggaagaga
    21901 agtgggtgtg ggattgttac ggaccgagcc atcgcgacaa cagcccggtg ttcggccgcc
    21961 tcgacgcggt cgtcgacttc accagcccga tgtggaaaga atcgctgcgt ttcgtcgagc
    22021 cgaacctgtg cggcgtcggc gggctgcatc tgatcccgac ctgcgagcag atgctcgccg
    22081 acgtcgtctt gcccgtgatt caggagtacg cgccgcagat tcagttggaa atcggccagg
    22141 acctgcgcga gttgttcatc gaggaggtgt tggatcatct ggatacgatt gggcggtccg
    22201 ggcgcaacat ctgcttcgtc gagccgaagt acgccgggga cggcccaaac gagcaggcgg
    22261 cgctcgccga atactatcac gagcggcacg ggctgaccgt gctgcacgcc gatccgtccg
    22321 agctgtatat gaagaaaggc gacgtgtggt acgaggacag cccggtcgac gtcgcctacc
    22381 gtgactacga ggtgcgcgac atcgtcgacc ttgaaagcga agggaccgac gtgcgcccca
    22441 tgcgcacgtt gttcaaagag aaccgcatgg tctcctccct ggcgggtgat ttcgatcaca
    22501 agagctgctg ggaagttctg agcgatccgc agttcaccaa taaatatttc aacgccgagg
    22561 agcgcctggt cttccgccgc cacattttgt ggacgcgtgt cctgtcggat cggcgcacgg
    22621 ccttgcccga cggcgaggtg gtgagcctgc tcgagcacac gcgcaagtac caggacgagc
    22681 tggtcgtcaa gcccaaccgg tcgtatggcg gcgaccgcgt gctgatcggg ccgtcggtca
    22741 gccagtcgga gtgggaagcg gccatcgaag aggcggtgac tggcgacgaa ccgtgggtcg
    22801 tgcagcgcct ggcgccgatc gccgtgaatg agtttccggt catcgccgag gatggggcgg
    22861 tgcagatgga gccgttctac acggtgctgg gcttcgcgcc gacccgctac ggcctggccg
    22921 tgctggggcg cgcgtcgcaa aagcaggtcg tcaacgtcgc gcagcggggc ggcatgtgcg
    22981 gcgtgctgat cggcaagcct tcaggacgcc tgatcgggcc gggcgcgccc gtgaggatga
    23041 gttagccatg gccaagactt cggaagtctt gaagagactt ccgaagtctg atgaaatgtc
    23101 gaagagcgtg ttccatgtca agagttccgt tcaatggtcg ccggccgggc atgttgggcc
    23161 gcgccgaaat tgaggacagg ctgaagtatg ccgtctatct ctcggaggcc gatgaaaccg
    23221 aagcggtgat tgaagcttcg gatttgtcgt tgacgcggtt cgctcgcaac gcgattcatc
    23281 agaacgtggc ggagacggat gccctgctcg aagtgcgcgc cgccttccag cggcgcgtgg
    23341 gcgcggcgtc gacgaacgac ctgtcccgaa tggggattta tcgggcagtc aagcgcgcct
    23401 gcgccctggc gcgtcacgcg ccggaaaacc ccaactggcc cggccttcca gagccgcagc
    23461 ctctgcccga ggtgcaggcg ttcgacgagg ccgtggcggg tataacaccc gaagggcggg
    23521 cgcgcgccgt ggcggacatt tgcggcgacg cgcgcgcgct caacgtggcg gcttccggcg
    23581 cgttcagcac cggctggtcg gagtacgccg tgatgaactc gaagggcctg ttcgcttatg
    23641 ccccgagcac tcaagtcgat ctgacgttcc tcgtcgagcg cccgcaggaa tcggcgtcgg
    23701 cgtacgccaa cgcgaccggc tggagattgt cgcagatcga cttccaggcg gtcaagcgtc
    23761 aggcgatcga gcatgcgctg gccgatcggc agccgcgccc tgtgccgccc ggcgaatatc
    23821 ccgtcgtcct cgagccgtat gccgtcgcga atctgctcga cgcgctgtcc gaggcgggca
    23881 tgggtgcgct cgccgtccag gaagagcgca gttggatgaa tgggcggatc ggtcgccgct
    23941 gtctctcgcc gaaggtgtct atcgtcgacg acgcgttcga cctcaacggg attcctcagg
    24001 cgttcgacgt cgagggcatg cccaagcgcc gcgtgccgat cgtcgtcgac ggagtgccga
    24061 cttcgccggt gtacgatcgc ctgacggcgg cgcgcgaacg ggaacgggcg tcgaccggcc
    24121 acgcccagcc tttcgacgac gactgggacg gcccattgcc cgagaatctt cacctggcgc
    24181 ccggcgacgt gaccgtgcag gagatgatcc gcgcgatcca ccgcggcctg tacatcacgc
    24241 gtttctggta cgtcaacctg accacgacgc acgagtgcgg cgtgaccggc acgacgcgcg
    24301 acggcgtgtg gtggatcgag cgcggcgagc tggcatatcc cgtgacggac atgcgtttcg
    24361 atcaggagct ggtccccgcg ctcgagcacg tgcgcggcgt ggggaacgat gtgcgcacgc
    24421 tgtccggctg ggccggcggc gtgcttcgcg tgcccgcgct ggcgctggag agtttccggt
    24481 tcatcgacgt gggtgacgat cgatacacag cgcgcaaata tgacgaggcg ggcgatgatt
    24541 aaccgatcat gcgcccgcgg catcgggtgg atttggaagc aggatattgg cggtgtagaa
    24601 tagaatgagt cgagcagatt ctgcgcttgc ggcgg
//
LOCUS       DBNHXB010000297         5878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3265220, whole genome shotgun sequence.
ACCESSION   DBNHXB010000297 DBNHXB010000000
VERSION     DBNHXB010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3265220"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..711
                     /locus_tag="AC162G_11965"
     CDS             124..711
                     /locus_tag="AC162G_11965"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR5076581.1"
                     /translation="MTAADHKALIEALFSGDDERAEEAALTLGRLGDAALPVLRDLLS
                     HPDGDCRWWATRALSEIRSPAAIAALIDQCADIDPDVRACAIYALGVLDGEAASAIPA
                     LIGRLADPSVYVGQLAADSLARIGPAATPRLIATLKEGTPSVRSRAARALAHIADPQS
                     IPALITALDDENPIVEYYADLALQKMGVGTVLLKV"
     gene            858..2522
                     /locus_tag="AC162G_11970"
     CDS             858..2522
                     /locus_tag="AC162G_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR5076582.1"
                     /translation="MSPDPITLEIFKHLFASVAEEMGVTLRRTAYSPNIKERLDFSCA
                     LFDGEGRLLAQAAHIPVHLGAMPASVRSAIEQCSPFAPGDVVILNDPYLGGTHLPDIT
                     LVSPVFIDAGAGLAPAHSGDRKGTPLLYVASRAHHADVGGMSPGSMPLSSEIYQEGLI
                     IPPVKIVDAGKANQAVLDLILRNVRTPDERRGDLDAQMAAHEVGARRAREIVARYGLQ
                     TTLAYAAALIEYAERLTRHAIASMPDGAYTFEDYLDDEELSNFKHQISNSQLPITNYQ
                     SPVTIKVILTIEGNSLSVDFTGSSPAVRGNLNAVRAIVESAVYYCVRCVVGAMGHDDA
                     PTNAGAFAPIRVIVPEGSVLDAKPPHAVAGGNVETSQRIVDAVFGALARALPDLIPAA
                     SQGTMNNLTFGGVRADGAPFAYYETMGGGAGAGPLRDGASGIHVHMSNTLNTPIEALE
                     LAFPLRVRQYAIRCGSGGAGRRRGGDGLIREIEFLAPVTATLLGERRAARPYGLSGGE
                     PGAPGENALIREGVTSRLPGKVQLQLDTGDVLSIRTPGGGGWGENE"
     gene            2526..4631
                     /locus_tag="AC162G_11975"
     CDS             2526..4631
                     /locus_tag="AC162G_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076583.1"
                     /translation="MLNVELCYNYFVVISLDSIVGELHLVRGQRQGVTPQCSAFTAPR
                     RSARGRQDDTLFILVDASGSASLIQELIQRIQQIYWHTSGSVTAGLRAAIDAGNEWLV
                     DNNRSAPAAAQAGVTCAVLRGPEVFIAQAGPSCAYLAHQGRLERFPRSDAAGSLPPLG
                     VARAVEVRFSRADLHPGDTLLLADASLPARIPEEAIASSIVYVGVETALDNLERLVRD
                     GNLIAMLIEVTAATAAPETSAAPAVAPATKTSIRDRVSPAPPRRIMPQVGEWAASVGQ
                     GVSRGLRSLSAAARGFVQTTLPDRPAARPARRRTAPSLESHTLIMAAIAIGIPLIVAA
                     VVTTVYVQRGAAAQVETLLAQGQASMAQADAAATGEEKRARWGDALAAANQALALDPN
                     NEVAIVLRTQAQAQLDQQDGTQRLSPILLYDYTKAGRRRLAVQGASLFVMDPDEGRVD
                     RFALSNQGDTVIGGEPAPAVSSGITVDDRVIGALIDMAWIEAGGQRQKSALVILERGG
                     LVEYDPAFGPSALPFAQDSAPPGVIRMDTFDGNIYLLDATGQQLWKYVATADGYTTLP
                     EGYFETAPPGIASTIDLAIDGNVYLLESNGVAHKYFGGEEATFQVSGLPSPIGKPVAA
                     AVDPYTPTDSGFYIADREGARIIHLAPDGRFIRQLRAPGGEFDALEDILVDERLGRLY
                     VISGGRLYSAALPSATSAP"
     gene            4827..5390
                     /locus_tag="AC162G_11980"
     CDS             4827..5390
                     /locus_tag="AC162G_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076584.1"
                     /translation="MSALFHIRQARNYPLEGCWAMRTWKDNGLTYVVVARRQPNGNIA
                     FGNYLVDYYCLGVKDAFCNAEIPSGEFRRDYLTKMFRGEKPLEISPALAHELVYGSIE
                     YAAQFGFKPHRDFKLAQHILDSADAHPRSGQVTFGKDGKPFFIAGPYDNVDAIVHQLL
                     RTAGADNFNYLVPLGPPSESLEIDDAI"
     gene            5465..>5878
                     /locus_tag="AC162G_11985"
     CDS             5465..>5878
                     /locus_tag="AC162G_11985"
                     /inference="COORDINATES: protein motif:HMM:NF016330.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HIR5076585.1"
                     /translation="MSRRRSKTDPFEDLTWDDLEDWAGSSVVSRGRSYQRSHHVQDLA
                     RTPGGGLIAWVLGTEKYATRVDFGEGGLTSACTCPYWTTCKHAVAVVLEYLESAKRKT
                     EIPIVTERDPRLADLRDVDEVGAEEWDEYDEGDAED"
BASE COUNT         1061 a         1945 c         1886 g          986 t
ORIGIN      
        1 tcatgctgat cgaagagggc gacaagtggc gtaatgtccc tgtcgagcaa ctgggacaac
       61 cttaacgtgt tgcgtgtttc gttttgaatg gcggaacacg caacacactt tcgagagccg
      121 atcatgaccg cggcagatca caaggccctg atcgaagcgt tgttttccgg cgatgacgaa
      181 cgcgccgaag aggcggcatt gacgctcggc aggctgggcg atgccgcctt gcccgtcctg
      241 cgcgatctgc tctctcatcc cgacggcgac tgccgctggt gggcgactcg tgcactcagc
      301 gaaatccgtt ctccggcggc gatcgcggcg ctgatcgacc aatgcgcgga catcgatccc
      361 gacgtgcgcg cctgcgccat ctacgcgctc ggcgtgctcg acggggaggc agccagcgcc
      421 atcccggctc tgatcgggcg gctcgccgac cccagcgtgt acgtcggcca gctcgccgcc
      481 gactccctcg cgcgcatcgg tccggcggcg actccccgtt taatcgccac cctgaaggaa
      541 ggcacgccct cggtgcgcag ccgcgcggcg cgggcgctgg cgcacatcgc cgacccacaa
      601 tccatccccg ccttgatcac cgcgctggac gacgaaaacc ccatcgtcga atactacgcc
      661 gacctcgcgc tgcagaaaat gggtgtcgga accgtgctgc ttaaagttta gggcgtagcg
      721 cgcatagcgt atcgcgtgtt gcgtgtcgcg cagtgtttaa cacagctcac gtttcacgca
      781 ccacgtatca cgttttacgc atcacgtttc acgtatcact cgccacgcat cacgcggctc
      841 ccaagcgatc tgactctatg tctcccgacc ccatcaccct cgagatcttc aagcacctgt
      901 tcgcctcggt cgccgaggag atgggtgtga cgctgcggcg cacggcgtac tcgcccaaca
      961 tcaaggagcg cctcgacttc tcgtgcgcgc tgttcgacgg cgagggccgc ctgctggcgc
     1021 aggccgcgca catccccgtg cacctcggcg cgatgcccgc ctcggtgcgg tcggcgatcg
     1081 agcagtgcag cccgttcgcg cccggcgacg tcgtcattct caacgatccc tatctcggcg
     1141 gcacgcactt gcccgacatc acgctcgtct cgccggtctt catcgacgca ggggcaggcc
     1201 tcgcgcctgc ccattcgggc gaccgcaagg gtacgcccct actgtatgtc gcttcgcgcg
     1261 cccatcatgc tgacgtcggc ggcatgtccc caggatcgat gccgctctcg agcgagatct
     1321 accaggaggg cctgatcatc ccgcccgtca agatcgtcga cgcgggcaag gccaaccagg
     1381 ccgtgctcga tctgatcctg cgcaacgtac gcacgcccga cgagcggcgc ggcgacctcg
     1441 acgcccaaat ggcggcgcac gaagtcggcg cgcggcgcgc gcgggaaatc gtcgcgcgct
     1501 acggcctgca gacgacgctg gcctatgcgg cggcgctgat cgaatacgcc gagcggctca
     1561 cgcgccacgc catcgcctcg atgccggatg gcgcttacac gttcgaagac tatctcgacg
     1621 acgaagagct ctccaatttc aaacatcaaa tttcaaactc ccaactccca attaccaatt
     1681 accagtcccc ggttaccatt aaggtcatcc tcaccatcga aggcaattcg ctcagtgtgg
     1741 atttcaccgg cagttcgccg gctgtgcgag gaaatctgaa tgccgtgcgc gcgatcgtcg
     1801 agagcgctgt gtattactgc gtgcgctgcg tcgtcggcgc gatgggtcac gacgacgcgc
     1861 cgacgaacgc cggcgccttt gcgccgattc gcgtgatcgt cccggaaggc tcagtgctcg
     1921 acgcgaagcc gccgcacgcc gtcgcgggcg gcaacgtcga aacgtcgcag cgcatcgtcg
     1981 acgccgtctt cggcgcgctg gctcgcgcgc ttcccgacct cattccggcc gcgagccagg
     2041 gcacgatgaa taacctgacg ttcggcggag tgcgggcgga cggcgcgccg ttcgcctact
     2101 acgagacgat ggggggtggc gctggcgcag gtccgctgcg cgacggcgcg tccggcatcc
     2161 acgtccacat gagcaatacg ctcaacacgc cgatcgaggc gctcgagctg gcctttcccc
     2221 tgcgcgtgcg gcagtacgcc atccggtgcg gctcgggcgg cgcggggcgg cgacggggcg
     2281 gcgacgggtt gatccgcgag atcgagttcc ttgcgccggt cacggcaacc ctgctcggcg
     2341 aacggcgtgc agcgcggccg tacggcctga gcggcggcga gcccggcgcg ccgggcgaga
     2401 acgcgctgat ccgcgagggc gtcacgtctc ggctccccgg caaagtccag ctccagctcg
     2461 acacgggcga cgtgctgtcg attcgaacgc caggtggcgg cggatgggga gagaacgaat
     2521 gaagaatgtt gaatgtcgaa ttatgctaca attacttcgt ggtgatctcc ctcgattcga
     2581 tcgtcggtga attgcatctc gtccgcgggc agcggcaggg cgtgacgccg caatgcagcg
     2641 cgttcacggc gccgcgtcgc agtgcgcgcg gacgccagga cgacacgcta ttcattctgg
     2701 tcgacgcgag cggatcggcc tcgctcattc aggagcttat ccagcgcatc cagcaaatat
     2761 actggcacac gtcgggcagc gtcaccgccg ggttgcgcgc ggcgatcgac gccggcaacg
     2821 agtggctggt cgacaacaac cggtcggcgc cggcggcggc gcaggccggc gtcacgtgcg
     2881 ccgtgctgcg cgggccggag gtgttcatcg cgcaggccgg gccgtcgtgc gcgtacctgg
     2941 cgcatcaagg tcgcctggag cgctttccgc gcagcgatgc agcgggcagc ctgccgccgc
     3001 tcggtgtagc gcgcgccgtc gaggtgcgct tctcgcgcgc cgatctgcac cccggcgaca
     3061 cactcctgct ggccgacgcc tcgctcccgg cgcgcatccc ggaagaggcc atcgcctcgt
     3121 cgatcgtgta tgtcggcgtc gaaacggcgc tcgacaacct ggagcgcctg gttcgcgacg
     3181 gaaacctaat cgccatgctg atcgaggtga cggcggccac agccgctccc gagacaagcg
     3241 ccgcgccggc tgtcgcgccc gcgacaaaga cttcgatacg tgatcgagtc tcccccgcgc
     3301 cgcctcgccg aatcatgcca caagtcggcg aatgggccgc ctcggtcggc cagggtgtgt
     3361 cacgcggtct gagatcgttg agcgcagcgg cgcgcggatt cgtgcagacg acacttcccg
     3421 atcggccggc ggcgcgcccg gctcgacgtc gcacagctcc ctcgctcgaa agtcacactc
     3481 tgatcatggc cgcgatcgcg atcggcattc cgctgatcgt cgccgccgtg gtgacgacgg
     3541 tgtacgtcca gcgcggcgca gcggcgcagg tcgagacgct cctggcacag gggcaggcat
     3601 caatggcgca ggcggatgcg gcggcgaccg gcgaagagaa gcgcgcgcgc tggggcgacg
     3661 cgctggctgc ggcgaaccag gcgctggctc tcgaccccaa caacgaagtc gcgatcgtgc
     3721 ttcgcacgca ggcgcaagca cagctcgacc agcaggacgg cacgcagcgc ctgtcaccca
     3781 tcctgctgta cgactatacg aaagcgggcc gccgtcggct cgccgtacag ggcgcgtcgc
     3841 tgttcgtgat ggacccggac gagggccgcg tcgatcggtt tgcgttgagc aatcagggag
     3901 acacggtgat cggcggcgag ccggcgccag ccgtatcgag cgggatcacc gtcgacgatc
     3961 gggtgatcgg cgcgctgatc gacatggcct ggatcgaagc cggcggccag cgccagaaaa
     4021 gcgcgctggt cattctggag cgcggcgggc tggtcgaata cgatccggca ttcggcccga
     4081 gcgcgctgcc cttcgcccag gacagtgcgc cgcccggcgt gattcgcatg gacaccttcg
     4141 acggcaatat ctacctgctc gatgcaacag ggcagcagtt gtggaaatac gttgcgacgg
     4201 cggatggcta tacgacctta cccgaaggct atttcgagac ggcgcctccc ggtattgcaa
     4261 gcacgatcga cctggcgatc gatggcaatg tgtacctgct cgaatcgaac ggcgtggcgc
     4321 ataagtactt cggtggagaa gaggcgacat ttcaggtgtc cggcctgccg tcgccgatcg
     4381 gcaagccagt cgcggcggcg gtcgatccgt acacgccgac cgatagcggc ttctacatcg
     4441 ccgatcgcga aggcgcgcgc atcattcatc tcgcgccgga cgggcgcttc atccggcagc
     4501 tccgcgcgcc cggcggcgaa ttcgacgcgc tcgaagacat cctcgtcgac gagcgtctcg
     4561 gccggctgta cgtgatcagc ggcgggcggt tgtattcggc ggcgctgccg tccgcgacct
     4621 cggcgccgtg acggggatag attttcacta ctactgacgg tgcggagaac gctggcaacc
     4681 ttggaatctg tgcgccctct gcacgccatc ggcgtccact gggaaagcag ccaatgaaaa
     4741 aaggcgagaa gaagaaatta aacaaagcac ataagcaacg cactcaggat aaactcgccc
     4801 gcaaacgcgc gcatgcggca gcgcctatga gcgccctgtt ccacatccgc caggcgcgca
     4861 actatcccct tgaaggttgc tgggcgatgc gcacctggaa ggacaacggc ctgacctacg
     4921 tcgtcgtggc gcggcgccag ccgaatggta acatcgcgtt cggcaactac ctggtggact
     4981 attactgcct gggcgtgaag gatgctttct gcaacgccga gattccttcg ggagagttca
     5041 ggcgcgacta cctgacgaag atgtttcgcg gcgagaagcc gctcgagatc tcgcccgccc
     5101 tggcccacga actcgtctac ggcagcatcg agtacgccgc gcagtttggc ttcaagccgc
     5161 accgtgattt caaactggca cagcacatcc tcgactcggc ggacgcacat ccgcgcagcg
     5221 ggcaggtcac gttcggcaaa gacggcaagc ccttcttcat agcggggcca tacgacaacg
     5281 tggacgctat tgtgcaccag ttgctgcgaa cggccggcgc agacaacttc aactacctgg
     5341 tgcccctcgg tccgccaagt gaatctctcg agatagacga cgctatctga agcttcgcgt
     5401 tattcgcgga caaagatccg cgtgtcgatg cacgccagca acatcattgc acaagagctg
     5461 tcaggtgagc agacgtcgct ccaaaaccga tccatttgag gacttgacct gggacgatct
     5521 cgaagattgg gccgggtcgt ccgtcgtttc ccgcgggcgc agctaccagc gcagtcatca
     5581 cgtgcaggac ctcgctcgca cacccggcgg cggtctgatc gcgtgggtgt tgggcaccga
     5641 gaaatacgcc acgcgcgtcg atttcggcga aggcgggctg acttccgcct gtacctgccc
     5701 ctactggaca acctgcaagc acgcggttgc cgtcgtgctc gaataccttg agagtgcgaa
     5761 gagaaagacc gagatcccca tcgtcacaga acgcgatccg cgcctggctg atctgcggga
     5821 tgttgatgaa gtgggagcgg aggaatggga cgaatatgac gaaggggatg cagaagat
//
LOCUS       DBNHXB010000298         5066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3265429, whole genome shotgun sequence.
ACCESSION   DBNHXB010000298 DBNHXB010000000
VERSION     DBNHXB010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3265429"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="AC162G_11990"
     CDS             complement(<1..188)
                     /locus_tag="AC162G_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076586.1"
                     /translation="MKSNPGRQPITLIAVLLVVGALMAGLVARLRRRPTLRSRPRGVV
                     ADQSGVFMTYQADRKALSS"
     gene            366..1127
                     /locus_tag="AC162G_11995"
     CDS             366..1127
                     /locus_tag="AC162G_11995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5076587.1"
                     /translation="MKIYGEVSTHIVQSQNRDPLLRYLTYMITKLAIRYVQNGGKILD
                     LGCGIGRTSVSLAKAGYDVTGLDVSEKAVNIARQIHKNGVAGSLHFIVGDETEPLSQK
                     FNERFDGVVCSEVIEHVEDYQSLLAVCRSVLKEGGFLILTTPNDPAQWTVLDDYAGHL
                     RRFEPRPLEEKLKSSDFVVAESFTVGFPFMRLLLKLYDYYIRKSRKKHDFKMYGRSVT
                     YQLIYIPLATLLLRVDSLFGWSKRGTTIVLVAQKR"
     gene            complement(1173..3260)
                     /locus_tag="AC162G_12000"
     CDS             complement(1173..3260)
                     /locus_tag="AC162G_12000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5076588.1"
                     /translation="MKILSILTYYYPHWTGLTAYAKRIGEGLAARGHRVTVLTTRHHH
                     SLPEEEVYNGVRIVRIKPIARISRGMICPAFPFAAHRLIREHDVIQIHTPLLESLLVA
                     LFCRRQGRPLVMTHHGDPVLPAGFINQSIERLLAGMMMFTERLADRISIHSRDYAEHS
                     DLLWPFASKLACIYPPVEIPEPQPEAAAAWRHELGLEHRKLIGFAGRFVEEKGFDYLL
                     QSIPMLLAAEPDVHLIYAGEHNVVYERFYERWKQLVESQRDRITFVGLLRDPQRLANF
                     YAMCDVFALPSRTDCFPSVQIEAMLCGTPVVTTNIPGAREAVRVTGMGRLAAPRNPVA
                     LAEGLIEVLRHPAHYTKPKAEIRAVFNTERTVDQYEALMAELAGLPAPAPRAAPPPRP
                     VAPPLSVSTVRYRNGTLPWVSLTERDHAILGRLLRNEADMAYRRRVPILLDYLELQDG
                     DQVFDCGCGMGFYLMVMGKLRRLRLVGLDGDLERLHWAQRERVPAALSSGDILRLPFA
                     DESFDKVLMSEVLEHLGDDRHALREIHRVLRPGGIVAFSVPHANYPFWWDPINRVWTS
                     LGGEPFRSGPLVGIWSNHERLYRPGELIERIQSAGFQVEIAEETTHFSFPFCHFLVYG
                     IGKPLIEHNLLPGGLRRSADRFMGEQNSGSLLNPINLGLSAFRAVDRLNNRPAVVSQE
                     TFVNILVKARKPE"
     gene            complement(3264..4280)
                     /locus_tag="AC162G_12005"
     CDS             complement(3264..4280)
                     /locus_tag="AC162G_12005"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR5076589.1"
                     /translation="MRKWTVWIGVAVSAVALLLAFRGVEWGELIRAASQADYAYLLPA
                     ALLMLAAIAARAERWRWLLGGRDRVGYWRSYRAVSIGYLMTNVLPFRLGELVRPIVIS
                     RGGAISAVHALSSIAVEHVLDVVMILALLAVVLPDLPLPPLVAQGARLSAVAFGGAAV
                     VMMLLVVAHKRVERAVVRFLDAISWIDTPTWLRRFNSAMEGLSVLRSPRLFVMSTLWT
                     LIGWLASVASLYCAMLAFVPAASLTSALFATVTSTLVLLVPSTPGYIGVLEWALRDSL
                     VLFGWSPEVALACAISFHFMELAVMNVSGVGCLVREGLSWSAMMSTVREAETRHDVAV
                     TVED"
     gene            4414..>5066
                     /locus_tag="AC162G_12010"
     CDS             4414..>5066
                     /locus_tag="AC162G_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR5076590.1"
                     /translation="MTKVRLDRLLVERGLVDSRERGQRLIMAGEVLVGDRVVDRPSQL
                     VQLDADIRVKTQLPYVSRGGFKLAAALEAFDIPVEGAVCADVGASTGGFTDVLLQRGA
                     ARVIAIDVGYGQLDWKLRSDPRVTVLDRTNARTLTELPRDSATGGPALARVVTIDVSF
                     ISLTLILPAVARWLPEGGHVVALIKPQFEAGRERVGKGGVVRDPGVHADVLRSVLGWA
                     "
BASE COUNT         1123 a         1512 c         1525 g          906 t
ORIGIN      
        1 gacgacagcg ctttccgatc tgcctggtaa gtcatgaaca cccctgattg atccgcgaca
       61 acgccgcgcg ggcgcgaccg tagggtcgga cgtcgccgca ggcgagcgac gaggcccgcc
      121 atcagcgcgc ccacaaccag cagcaccgct atcagcgtga tgggctgacg accaggattg
      181 cttttcatcg cctaatccga tagtctctag ggaaagtgta ccagcaaacc gctgcattat
      241 ccataacgga taccccggac agcacgccag ttgttgaagc agagacgccg aaggaagatt
      301 cggcctgggc aatccaggta taatggtact ttagaccgtg tgacgacggt gaggaaggtt
      361 aagtcatgaa aatttatggc gaagtcagta ctcatatcgt acaatctcaa aatcgagacc
      421 cactactcag atatctgacg tacatgatta ccaaattggc aatacggtac gtccaaaatg
      481 gtgggaagat cctggatcta ggatgcggta ttggccggac gagtgtgtcg ctggccaagg
      541 caggatatga cgttacgggt ttagatgtca gtgaaaaggc ggttaacatt gcacgtcaaa
      601 ttcataaaaa tggtgtggct ggctcgcttc atttcatcgt cggcgatgag acggaaccgt
      661 tgagccagaa gttcaatgag cgatttgatg gagttgtctg ttctgaggtc atcgaacatg
      721 tcgaggatta ccagagcttg ttagccgttt gtagaagcgt tcttaaagaa ggcggctttc
      781 ttattctgac gaccccaaat gacccagccc agtggactgt actcgatgat tatgcaggac
      841 atctccggcg cttcgagcct cgaccactgg aagaaaaact caaatcctcg gattttgttg
      901 tcgccgagtc gtttaccgtc gggttccctt ttatgcgact acttctaaag ctctatgact
      961 attatattag aaagagtagg aagaaacatg actttaagat gtatgggcga agtgtgactt
     1021 accaattaat ctacattccc cttgccactt tattgcttcg agtcgatagt ttgttcggtt
     1081 ggagcaaaag aggaacgact atcgttctgg tcgcacaaaa aagatgagcg tcgggctaga
     1141 gaaacctggt tttctaaagg cggccagtac actcactccg gtttgcgcgc tttgacgaga
     1201 atattcacaa aggtctcctg gctgactacg gctggccggt tattgagccg gtcaactgcg
     1261 cgaaaggcag acagacccag gttaatgggg ttgagcaggc tgccgctatt ctgctcgccc
     1321 atgaaccggt cggcacttct gcgcagtccg cccggcagca agttgtgctc gatgagcggc
     1381 ttgccgatgc catacaccag aaagtggcag aaggggaagc taaagtgagt tgtctcctcg
     1441 gcaatctcaa cctgaaagcc agcgctctgg atgcgctcga tcagctcgcc gggccgatac
     1501 agtctctcat ggtttgacca aatgcccacg agtgggccgc tgcgaaacgg ctcaccgccg
     1561 aggcttgtcc atacgcgatt gatcgggtcc caccaaaacg gatagttggc gtggggcacg
     1621 gagaaggcca cgataccgcc gggccgcaag acgcgatgaa tctcgcggag cgcgtgccgg
     1681 tcatcaccca ggtgttcaag cacttcggac atcagcactt tatcaaaaga ttcatcggcg
     1741 aaggggagcc taaggatgtc gccgctcgac aatgcggcgg gtacgcgttc acgctgcgcc
     1801 cagtgcagcc gctcaaggtc accgtccagc cccaccagcc gcaatctccg cagcttaccc
     1861 ataaccatca ggtaaaaacc catcccgcag ccacaatcga agacttgatc gccatcctgg
     1921 agctccaggt aatccaacag aatagggaca cgccgccgat aggccatatc cgcctcgttg
     1981 cgcaagagcc gtccgaggat cgcatgatcg cgttcggtga gcgacaccca gggcagcgtg
     2041 ccgttgcgat agcggacagt agacacgctc aacgggggcg ccacgggccg aggcggtggc
     2101 gccgcgcgcg gcgccggcgc tggcaaacca gccagctctg ccatcagcgc ctcatactga
     2161 tcgacggtgc gctccgtatt gaagacagcc cgaatttcgg ctttgggttt ggtgtagtga
     2221 gccggatgcc gcaggacctc gattaacccc tctgccagcg caaccgggtt gcgaggcgca
     2281 gccaggcgtc ccatcccggt gacccgcaca gcctcccgag caccagggat gttggtcgtc
     2341 acaacgggtg tgccgcatag catcgcttca atctggacgc tgggaaagca atcggtgcga
     2401 ctgggcagtg cgaagacatc acacatggca tagaagttgg caagacgctg tgggtcacgc
     2461 aggagaccga caaaggtgat gcgatcacgc tggctctcga cgagttgctt ccaccgctcg
     2521 taaaagcgct catacacgac gttgtgctca cccgcgtata tcaagtgaac atccggctcg
     2581 gcagccagca gcatggggat agactgaagc aggtaatcga agcctttctc ttctacaaag
     2641 cgcccggcga aaccgattaa tttcctatgc tccagcccca actcgtgacg ccaggcggca
     2701 gccgcctccg gctgtggctc gggaatctcg accggtggat aaatgcaggc cagcttggag
     2761 gcaaagggcc acagtaagtc agagtgttca gcgtagtcac ggctgtgaat actgattcgg
     2821 tcggccaggc gctccgtgaa catcatcata cccgccagca ggcgctcaat cgactgattg
     2881 ataaacccgg caggcagcac agggtcgccg tgatgggtca tcactaatgg ccgcccttgt
     2941 ctacggcaga aaagcgccac cagcagcgac tccaacagcg gcgtatggat ctgaatcaca
     3001 tcatgctcgc gaatcagacg atgagccgcg aacggaaacg cggggcagat catgccgcgg
     3061 ctgatgcgcg caatcggctt gatgcgcaca atgcgaacac cattgtagac ttcctcctcc
     3121 ggcaaggagt ggtgatgtcg agtcgtgagg acagtgaccc ggtgtccacg agccgccagg
     3181 ccctccccaa tgcgcttggc gtaagccgtc agtcccgtcc agtgaggata ataataagta
     3241 aggatagaca gaatcttcat gcttcaatcc tcgactgtca ccgccacatc gtgtctggtt
     3301 tcggcctcgc gcacggtgga catcatcgcc gaccacgaca ggccctcgcg gacgagacag
     3361 ccgacgcccg agacgttcat cacggccagc tccatgaagt gaaagctgat ggcgcaggcc
     3421 agcgccacct cgggactcca gccaaagaga accagcgagt cgcgaagcgc ccactccagc
     3481 acgccgatgt aacccggcgt cgacggcacc agcagcacga gcgtcgacgt gacggtggcg
     3541 aataaggcgg aagtcaacga tgcggcgggg acgaaggcga gcatcgcgca atagagcgac
     3601 gcaaccgacg ccagccagcc gatgagtgtc cagagcgtcg acatgacgaa caggcgcggg
     3661 ctgcgcagaa cgcttagccc ttccatcgcc gagttaaagc gccgcagcca ggtaggggtg
     3721 tcgatccagg aaatggcgtc gaggaagcgt acgaccgcgc gctcgacgcg cttatgcgcg
     3781 acgacgagca gcatcatgac gaccgccgcg ccgccgaaag cgaccgcact gagacgtgca
     3841 ccctgggcca cgagcggtgg cagcggtaga tcaggcagca ccacagccag cagcgccagg
     3901 atcatcacga cgtccagcac gtgctcgacc gcgatcgacg acagggcatg cacggcgctg
     3961 atcgcgcccc cccgcgagat gacgatcggg cgcaccagct cgccgagccg aaacggcagc
     4021 acgttcgtca tcaaataacc gatcgagacg gcgcggtacg agcgccaata gccgactctg
     4081 tcccgcccgc ccaacagcca gcgccagcgc tcggcgcgcg ccgcgatagc cgccagcatg
     4141 agcagcgccg ccgggagcag gtacgcataa tccgcctggc tcgccgcgcg gatcagctcg
     4201 ccccactcaa cgcctcggaa ggccagcagc agcgccacgg cgctgactgc cacgccaatc
     4261 cacacagtcc atttgcgcac gaccatctcc acacaataag cggtggctgg ggccaccgcc
     4321 gcttcgagcg cattataaca tctcgatcct gtccccgcaa aatgccctcg cggacagcct
     4381 tgacttttgc ccgacttgga atatgcttgt actgtgacga aggtccggct ggatcggctg
     4441 ctcgtcgagc gagggctggt ggacagccgc gagcgcggtc agcgtttgat catggccggc
     4501 gaagtcttgg tcggcgatcg cgtcgtcgac cggcccagcc agctcgttca gctcgacgcc
     4561 gatatccgcg tcaaaacgca attgccgtac gtgagccggg gcggtttcaa gctggctgcg
     4621 gcgctggagg cattcgacat cccggtcgag ggcgcggtat gtgccgacgt gggcgcctcg
     4681 acgggtggct tcaccgacgt gctactgcag cgcggcgcgg cgcgcgtgat cgccatcgac
     4741 gtcggatacg gccagctcga ctggaagctg cgcagcgatc cgcgcgtgac cgtgctcgat
     4801 cgaacgaacg cgcgcacgct gaccgagctg ccgcgcgatt cggcgaccgg cgggccggcg
     4861 ctcgcccgcg tcgtcacgat cgacgtatcg tttatctcgc tgacgctcat cctgccggcc
     4921 gtggcgcggt ggctgccgga gggcgggcac gtcgtggcgc tgatcaagcc gcagttcgaa
     4981 gcggggcgtg agcgggtagg caaaggcggc gtggtgcgcg atccgggcgt gcacgccgac
     5041 gtgctgagat cggttctggg gtgggc
//
LOCUS       DBNHXB010000299         7898 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_246338, whole genome shotgun sequence.
ACCESSION   DBNHXB010000299 DBNHXB010000000
VERSION     DBNHXB010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7898)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7898)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7898
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_246338"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="AC162G_12015"
     CDS             complement(<1..1021)
                     /locus_tag="AC162G_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076591.1"
                     /translation="MKRLRPLAAVMFTMLLAACMDGTPTLQASPSASAVPQIVAFEVF
                     PNPVERGDELRVTWQVDGASRAALWPMTYDSKLGRWYRQRDPISTGSTAGEYRLTVPP
                     DADRTLRFELEATDTIGNSVTATSDEIRLVCHPIFFDLSRTAWCPNPPQTTLAAFQAF
                     EGGYMIWRADTGQVYMLLQPAEPELPDGWFASVPTSKAVTVDAPPGRYAPGEHFQRAW
                     ASLTEHWRSLGWGVAPEQAFTLTAQLSLSSGDTMSQNDDLYLSWPDGRIAHLRVYLSA
                     PNHASGPAWSFVDPNTVPAPAVDASAVPTPFPTVAAKAPTILSFTADRVNAKPGDRVT
                     LSWTST"
     gene            complement(1034..1198)
                     /locus_tag="AC162G_12020"
     CDS             complement(1034..1198)
                     /locus_tag="AC162G_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076592.1"
                     /translation="MNQSLLRSNEMSPAAADPMAVYALAAEGDVRWAGTSFGVCAPNG
                     RGSRCSPSGI"
     gene            complement(1206..2441)
                     /locus_tag="AC162G_12025"
     CDS             complement(1206..2441)
                     /locus_tag="AC162G_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR5076593.1"
                     /translation="MTVLTHQQVRYALHVGRESFDAGAQSELKQHLVECTACRTYAAE
                     LTTLQASLSRVMRARWESHRPPVDSIPRIWARVDRKAAQRRVLRFGGSWVTTGALAIL
                     AGVLIWMISVTGPSNENPGGQSAAGRASGSAVPVPTPVVIVPTPIPGLAIFGGNITLI
                     EGDLAETRFMAGSTISLTLHWQAHAAMTTSYNVSVHVLDVEGNLVAQSDSIPGDGAHP
                     TTNWLPGETVADQHQFVLPDSLATGRYELAAGVYDAANGARLLTDNGESIVRFATIDV
                     QGISHRVGEDFGEFATLLGFDLASDRLAAGDAVEMTLYWRARAQTATSYTVSVQVFGE
                     NKTIVAQADSVPDGGARPTTNWQPGEVIADRYTIRLPANLPPGEYRVVMGLYDSASGI
                     RLSTPADNQSVVLATLQVE"
     gene            complement(2438..3001)
                     /locus_tag="AC162G_12030"
     CDS             complement(2438..3001)
                     /locus_tag="AC162G_12030"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5076594.1"
                     /translation="MEYSDTLQLIGLCRTGDSQAIESLVRRHQSLIYRLAVSILDDPA
                     EADEATQDAFVAAIDRLDSFRGDSSFTTWLYAIALNVCRGRLRRRRVRERMMEAVHAL
                     FRVSGEAEAYPEQIVAQREADTALWQAIQALDDRLREVVILRYYHELRLNDIAQITGV
                     SERSVRNRLHAAHERLRIRLDERSHLS"
     gene            complement(3265..4872)
                     /locus_tag="AC162G_12035"
     CDS             complement(3265..4872)
                     /locus_tag="AC162G_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076595.1"
                     /translation="MNKHLLIVLGFSIVLSACSALPRELDRVADRLADATLRAPTAEP
                     RPTDPVATPTARSWPPPTSTSASILPDGVSISLSPAVWLGGNYYQVSGPATVSALAPG
                     AVARVEFYTAPTGTGVTPRLESADTDGRDGWSWYWQTPLPGSHLWAEAIYADGRRNSS
                     AYLMITASEGEPPAWQTYANADLGYSLEYPSNWQVDEYGLSQLSKEVLFSPPDPEPFI
                     AYFSISLDGRTLEQIQQSYAEQFPNVSPEWIGFAGQRALQYRFSETRFERYVPLHHGV
                     VRFASDRATLPEVQHMLSSFRVIVPIGTVPPPTPACETHTASVQVLPSAQSVQIGQQL
                     IVRVTLLNQGCVALGLPKYTLRAHAPQGQPLFNPPQPESVTHGLAVAPGQSDTVEFVL
                     QGAQVGSGALQADVSFEVHIGYPGPAYWGYASSGPINIAVTGNHKPPDSERPAAGICA
                     EVVDGIASITIGPDVPSPRCVKVTTNVRLKFVNATDAAVQVRLWSFDAQIAPGGEQII
                     DAPVGSYLMPGVHFIEGIGAEVWLVGS"
     gene            complement(5121..5552)
                     /locus_tag="AC162G_12040"
     CDS             complement(5121..5552)
                     /locus_tag="AC162G_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076596.1"
                     /translation="MTSAQQGIARLASRLALIGGLIQALVALGLLLLPVFTTCIYVDG
                     APECHSQSYLQQGGSLVGYLILTLAILAGVVAVLSSQDLNSPKAVLSRWLAASAGVIV
                     VAVGIWSIGIVFMPGTLVILLSALLTRPGRGADRTHEAKDA"
     gene            complement(5580..6209)
                     /locus_tag="AC162G_12045"
     CDS             complement(5580..6209)
                     /locus_tag="AC162G_12045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024852.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5076597.1"
                     /translation="MRLVIFGASGRTGRHLIMQALEQGHTVTAFARTPGKIQTNHARL
                     HIVQGNVLDAMAVEKAVAGHDAVLCALGVNRDDPTTALAEGTRHILQAMEKHGVRRMV
                     CVSAAGFLGEQADFLIGKILFWYFGRFLTRLFDAMKRQYQELEKSAVDWIAVRPLLLD
                     EGPRRGNYRIAVVGIPSRGYRINTGDVAEFMLQQLTNGEYVRKAPAIAY"
     gene            complement(6274..7224)
                     /locus_tag="AC162G_12050"
     CDS             complement(6274..7224)
                     /locus_tag="AC162G_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076598.1"
                     /translation="MAEQPHTEIVETTRLRSMHLLRLLLTIAILMQVACVTPGVDGGP
                     PPAVSTVAPAHTAAAHSSTPLASPVEVLTPNPAPTSDPNSLAPQSNHIYLATLSGERV
                     LFVTNTNFQRSFDADGAVTIDPDLGLMVNEDGQGRSPSGFQLLEQPRLVYVAQHDVTW
                     LNFKFDPTGRLMVVSLWYPGADGAHSFLNHVIEIDFDDLTSRILWTYQNGDDRYPGFD
                     GGALIDEIVGHIVVLPLFPCNACDAFTPHAIVLLNVTTGNELYLGTVGDIRIDVAGNV
                     VSYRNLAESRVPCEPSPGCDNDGLRLVYEPAGETLTAQLP"
     gene            complement(7264..>7898)
                     /locus_tag="AC162G_12055"
     CDS             complement(7264..>7898)
                     /locus_tag="AC162G_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ligand-binding sensor domain-containing protein"
                     /protein_id="HIR5076599.1"
                     /translation="GQTWTTYSTTNGLGGNHVADVEFAPNGAVWVGTDGGVSRFDGQM
                     WTTYTTANGLPEDWVDELAIAPDGAVWTITHSRVSRFDGHSWRSYTTADGLSSDYVSH
                     LTLAPDGAVWVKDGDGVSRFDGQAWRNYTAADGLANFSLYGMMIAIAPDGSVWVGSDK
                     GVSRFNGQTWTTYTTSDGLATNRITGFAFAPDGSVWVSMDLGVARFSGSP"
BASE COUNT         1555 a         2453 c         2410 g         1480 t
ORIGIN      
        1 ctgtcgacgt ccaggataat gtaacgcgat cgccgggctt cgcattcacg cgatcggcgg
       61 tgaagctcag gatcgtcggc gctttcgcgg ctacggtcgg aaacggcgtc ggcacagcag
      121 atgcgtcaac ggcgggcgct ggcaccgtgt tggggtcgac gaaggaccag gctggaccac
      181 tggcatggtt gggcgcggac agatacacgc gcaggtgagc gatccgtcca tccggccacg
      241 aaagatacag atcgtcgttc tggctcatcg tgtcgcctga actcaggcta agctgcgcgg
      301 tcagggtgaa ggcctgctcc ggtgccacgc cccaacccag cgagcgccaa tgctcagtca
      361 gactcgccca cgcgcgctgg aagtgttcgc cgggcgcata ccggccaggc ggcgcgtcaa
      421 ctgtcaccgc cttactcgtg ggtacagagg cgaaccatcc gtccggcaat tcaggctccg
      481 ccggctgcaa tagcatgtac acctgccccg tgtcggcgcg ccagatcatg tagccacctt
      541 cgaacgcctg aaaggcggcc agggtggtct gcggcgggtt cggacaccac gccgtccggg
      601 ataagtcaaa aaagatcgga tgacacacca gccggatctc atcgctcgtc gcggtcacgc
      661 tgttgccaat tgtgtccgtc gcttcaagtt caaagcgcag tgtccggtcg gcgtcgggcg
      721 gcacggtcag acgatactcg ccggcagtcg atccggtcga gataggatcg cgttgccgat
      781 accatcgtcc gagtttcgag tcgtaggtca tcggccacaa cgcggcgcgc gacgcgccgt
      841 cgacctgcca ggtgacacgc agttcgtcgc cacgctccac agggttggga aaaacctcga
      901 aagcgacgat ctgcgggacg gcggacgcgg agggagacgc ctgcagcgtc ggcgttccat
      961 ccatacaggc tgccagtaac attgtgaaca tgaccgctgc gagcggtcgg aggcgtttca
     1021 taacttcaca ccttcagatt ccagacggcg aacatctcga cccacggcca ttcggcgcgc
     1081 acacgccgaa cgaggtgccc gcccaccgca cgtcgccctc cgccgccagt gcgtacacgg
     1141 ccatgggatc ggcggccgcg ggcgacatct cgttcgaacg aagcagagac tgattcatgc
     1201 tgccgctatt ccacctggag tgttgccagc acaaccgact gattgtccgc aggtgtgctc
     1261 agccgtatcc cgctcgctga gtcgtacagg cccatcacca ctcgatactc tcccggcggc
     1321 aagttggcgg gcagccgaat cgtgtaccgg tcggcgatga cctcgccggg ctgccaattc
     1381 gtcgtcgggc gcgcgccgcc atccggcaca ctgtcggcct gcgccacgat cgtcttgttc
     1441 tcaccgaaga cctgcaccga gacggtgtac gatgtcgccg tttgggcgcg cgcgcgccag
     1501 tacaacgtca tctcgacggc gtcgccggcc gcgaggcgat cgctcgccag atcgaagccg
     1561 agcagtgtgg caaactcgcc gaagtcttcg ccaacccgat gggagatgcc ctgcacgtcg
     1621 atggtcgcga accggacgat gctttctcca ttgtctgtga gcagccgcgc tccgttggcg
     1681 gcgtcataca caccggccgc gagttcgtag cgtcccgtcg ccagactatc cggcagcacg
     1741 aattgatgct gatccgcgac ggtctcgccg ggcagccaat tcgttgtagg gtgcgcgcca
     1801 tcgccgggaa ttgaatcgga ctgcgcgacg aggttgccct cgacgtcgag cacgtgcacg
     1861 gaaacgttat acgaggtcgt catcgccgca tgcgcttgcc aatgcagggt cagactgatc
     1921 gtgctgccgg ccatgaaacg agtctccgcc agatcgcctt cgatcaacgt gatgttcccg
     1981 ccgaaaatgg ccagccccgg aatcggcgtc ggcacaatga cgacaggggt gggaaccggc
     2041 acggccgacc cggacgccct tccggccgcc gactgcccac ctgggttctc gttgctcggt
     2101 ccggtgacgc tgatcatcca gatgagcacg ccggccagga tggccaacgc gccggtcgtc
     2161 acccatgatc cgccgaagcg gagaacgcgc cgctgcgccg ccttcctgtc gacgcgcgcc
     2221 cagatgcgcg ggatcgagtc aaccggcggg cgatgcgact cccaccgcgc gcgcatcacg
     2281 cgtgacaggc tcgcctgaag cgttgtcagt tcggcggcat aggttcggca agccgtacac
     2341 tcgacgaggt gctgcttcag ttctgactgc gcgccggcgt cgaacgactc acgtccaaca
     2401 tgcagagcgt agcgcacttg ctggtgtgtc agcacagtca tgacaggtgg ctcctttcat
     2461 ccaagcggat tcgcagccgc tcgtgcgcgg catgcagccg gttgcgcacg ctgcgctcac
     2521 tgacacccgt gatctgcgcg atgtcgttca gacgcaattc gtggtagtag cgcagaatca
     2581 ctacctcgcg caatcgatca tccaaggcct gtatggcctg ccacaacgcc gtgtcggctt
     2641 cacgctgcgc gacgatttgc tccggatagg cttcggcttc gccgcttacc cggaacaggg
     2701 cgtgcacggc ttccatcatt cgctcgcgaa cgcggcgcct gcgcaaccgc ccccggcaca
     2761 cgttcaacgc aatcgcgtac agccaggttg tgaaggacga gtcaccacga aaggaatcga
     2821 gccggtcgat ggcggcgaca aaggcatcct gtgtcgcttc atccgcctcg gccggatcgt
     2881 cgaggatgga gacggccagg cggtagatga gcgattgatg gcgacgcacc agcgattcta
     2941 tggcctgtga atcgccagtg cggcacaggc cgatcaattg aagagtatcc gagtattcca
     3001 ttctactccc gtgctcgtga tgcggcgttt ggccagttca agggataatc aaaaggaagg
     3061 tgtttgagtg ttcctgtgtg ctaataagat gcgcgtcagg cttgataccg gaagacgagt
     3121 atttgggccg cggcgcggta agcggccgcg tgcaacgacg ggttgggcgc acgtgcctga
     3181 tacaatttct cgattcccga cgccggaggt tggcgacggt gacggcccga ccacagtcgt
     3241 ctgagaactg tcttcgagcc agtctcacga gccgaccagc cacacttctg cgccgatgcc
     3301 ctcgatgaag tgcacgcccg gcatgagata cgagccgacc ggcgcgtcga tgatttgttc
     3361 gccgccgggc gcgatctgcg cgtcgaaact ccaaagcctg acctgcaccg ctgcgtccgt
     3421 cgcgttgacg aacttcagcc ttacgttggt cgtgaccttc acacagcgcg gcgagggcac
     3481 gtcgggacca atcgtgatgg atgcgatgcc gtccacaacc tccgcgcaaa tgccggccgc
     3541 cggccgctcg ctgtcgggtg gcttgtggtt accggtcacc gcgatgttga tcggtccgct
     3601 gcttgcatag ccccagtaag ccggaccggg ataaccgatg tgcacctcaa atgaaacgtc
     3661 cgcttgaagt gcgcctgacc caacctgggc accttgcagc acaaactcca cggtatcgga
     3721 ctggcccggc gcaacggcca aaccatgcgt caccgattcc ggctgaggcg gattgaacag
     3781 gggctgtccc tggggcgcgt gagcgcgcag cgtgtatttg ggcaggccaa gtgccacaca
     3841 gccctggttc aacaaagtca cacgcacgat cagttgctgg ccgatctgca cgctttgcgc
     3901 cgacggtaat acctgcacgg aagccgtatg tgtttcacac gctggcgtcg gaggcggaac
     3961 agttccgatg gggacaataa cgcgaaagct gctcagcatg tgctgcactt cgggcagcgt
     4021 ggctcgatcg gaggcgaacc gcactacccc atgatgcagc ggcacgtatc gctcgaagcg
     4081 ggtttcgctg aagcgatatt gcagcgcacg ctgccccgca aaaccgatcc actctggcga
     4141 cacgttggga aactgttcgg cgtaggattg ctggatttgc tccagtgttc gtccatcgag
     4201 tgagatgctg aaatatgcga tgaacggttc gggatcgggc gggctgaaca acacctcttt
     4261 ggagagctgc gaaaggccgt attcgtcaac ctgccagttg gatggatact ccagtgagta
     4321 gccgagatcg gcgttggcgt aggtctgcca cgcaggcggc tcgccttcag atgccgtaat
     4381 catgagataa gcagaagagt ttcgcctgcc atcggcgtag atcgcttcgg cccatagatg
     4441 cgatcctgga agcggtgtct gccagtacca tgaccagcca tcgcggccgt cggtgtcggc
     4501 agactccagt cgcggcgtga cgcccgtgcc ggtgggcgcg gtgtagaact cgacgcgcgc
     4561 caccgcgcca ggcgcgaggg ctgataccgt cgccgggcct gagacctggt agtagttccc
     4621 gcccagccac actgccggtg acagcgagat gctgacccca tcgggaagga tcgatgcaga
     4681 cgtgctcgtc ggcggcggcc acgaccgtgc tgtcggcgtg gctactggat cggtggggcg
     4741 cggttcagcg gtcggcgcgc gcagagtggc atcggccaga cgatcggcca cgcgatccaa
     4801 ctcacgcggc agcgcgctgc acgcgcttaa tacgatcgaa aagcccagga cgatcaacag
     4861 atgtttgttc atatcgcgta tgaccccact atcgtgattt ccccttcgag tatgttcgta
     4921 cgggtgtttt cagcacatca aggaagccag acaggctcgc agccttcaga cgccagcagg
     4981 gcaccgccgg ttccatcgac gctgatcacg taggctttgt gcccgacgac gcgatgcccg
     5041 gaaccctgcc caatgttttc tccacccctg gtatagactc gatgggccga gcgctcactt
     5101 gaccttcccc tgagcatcgg tcacgcatcc ttcgcctcgt gtgtgcgatc ggccccccgg
     5161 ccaggccggg tgagaagtgc ggacagtagg atcaccagcg ttcccggcat aaaaacgatc
     5221 ccgatgctcc agattccgac ggcgacgacg atgacgccgg cagacgcggc cagccagcga
     5281 ctcaacacag ccttgggaga gttgagatcc tggctgctga gtaccgccac gacgccggcc
     5341 agaatcgcaa gtgtcaagat caagtagccc accaaagatc cgccttgctg gagataggac
     5401 tggctgtggc actcgggcgc accatcgaca tagatacagg tcgtgaagac aggcagaagg
     5461 agcaggccga gcgcaaccag tgcctgtatg aggccgccaa tgagagcaag ccgcgaagct
     5521 aaccgggcta ttccttgctg tgcagaggtc atacgccatc gccttcttat tcgtccacct
     5581 caatacgcaa tcgccggtgc ctttcgtaca tactcaccgt tcgtcagttg ttggagcata
     5641 aactcggcca catcgcccgt gttgatacga taaccccggc tgggaatgcc gacgactgca
     5701 atgcgataat tgccccggcg cggcccctcg tccagcaaga gggggcgaac cgcaatccag
     5761 tccaccgcgc tcttctcgag ttcttgatat tgccgtttca tcgcgtcgaa tagcctggtc
     5821 aagaacctcc cgaagtacca gaacagtatc ttgcctatga ggaaatcggc ttgctcgccc
     5881 aagaaaccgg ctgccgatac gcagaccatt cgccggacgc catgtttttc catggcttga
     5941 aggatatggc gcgtaccctc tgccagggca gtcgtcggat catcgcggtt gacgcccagt
     6001 gcacacagga cagcgtcgtg accggcaaca gccttctcca cagccatcgc gtccaggaca
     6061 ttgccttgca cgatatgcaa tcgtgcgtgg ttcgtttgta tcttgccggg cgtacgggcg
     6121 aaggcagtaa ccgtgtggcc ttgttccaat gcctgcataa tcagatggcg tcctgttctt
     6181 cccgatgctc caaagatgac gagtctcatg gcttacttat tcctgggttc tcgatgaatg
     6241 gtgcagcggg gcggcacttt gaaaccattc agttcagggt agttgagccg ttagcgtttc
     6301 tccggcaggt tcgtagacga gccggaggcc atcattgtcg caccccggac tcggctcgca
     6361 gggcaccctg ctctcagcca ggtttcgata cgagaccacg ttccccgcca catcgatccg
     6421 gatgtcgccc accgtgccga gatacaactc gtttccagtg gtcacattca gcagcacaat
     6481 cgcgtgcggc gtgaatgcat cgcaggcgtt gcaggggaac agtggcagca ccactatgtg
     6541 accgacaatt tcatcgatca gcgcaccacc gtcgaagccg gggtagcgat cgtcgccgtt
     6601 ctggtacgtc cacaatatcc ggctggtcag gtcgtcgaag tcgatctcga tgacatgatt
     6661 gagaaacgaa tgtgcgccgt ccgcgccggg ataccagagc gagacaacca tcaggcggcc
     6721 tgtcgggtcg aacttgaagt tgagccacgt gacatcgtgt tgagcaacat agaccaagcg
     6781 gggttgttcc aggagctgaa agcccgacgg gctgcgacct tggccgtctt cgttgaccat
     6841 cagccccagg tcaggatcga tggtgacagc accgtccgca tcgaaggagc gttggaagtt
     6901 cgtgttggtg acgaacagga cgcgctcgcc agagagcgtg gcaaggtata tgtggttcga
     6961 ctgaggcgcc agagagtttg gatctgaagt cggcgccggg ttgggcgtga gcacttccac
     7021 cggagatgcc aacggagtcg atgagtgtgc cgctgcagtg tgcgcaggcg caacggtgct
     7081 cactgccggt ggcggcccgc cgtccacgcc cggcgtgacg caggccacct gcataaggat
     7141 cgcgatcgtc aagagcagcc tgagcaaatg catgctgcgc aggcgagtag tctccacaat
     7201 ctctgtgtgc ggctgttctg ccatgaacac ttccttggtt gaaacagttc agtcttcaaa
     7261 gagtcaggga ctgccactga atcgcgcgac acccagatcc atgctcaccc atacgcttcc
     7321 atcaggagcg aaagcgaacc cggtaatacg attcgtggcg agaccatctg acgttgtgta
     7381 cgtcgtccag gtctggccgt tgaagcggga cacgcccttg tccgaaccga cccacacgct
     7441 gccatcgggg gcgatggcga tcatcatccc gtagagagaa aagttggcca ggccatcggc
     7501 agcagtgtag tttctccagg cctggccgtc gaaacgcgag acgccatcgc cgtctttgac
     7561 ccacaccgcg ccatcgggtg ccaaggtgag atggctgaca tagtcgctgg acaggccatc
     7621 cgccgtggta tagcttcgcc acgaatgccc atcaaagcgg gatactctgc tgtgtgtgat
     7681 ggtccacacc gcgccatcgg gggcgatggc aagttcgtcg acccaatcct caggcaggcc
     7741 attagccgtc gtataggtcg tccacatctg gccgtcgaag cgagatacgc cgccgtccgt
     7801 gccgacccac actgcgccat ttggagcgaa ctcaacatcg gcgacgtgat taccccccaa
     7861 gccgtttgtc gtcgagtaag tggtccaggt ctggccgt
//
LOCUS       DBNHXB010000300         2742 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2594936, whole genome shotgun sequence.
ACCESSION   DBNHXB010000300 DBNHXB010000000
VERSION     DBNHXB010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2594936"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..123
                     /locus_tag="AC162G_12060"
                     /pseudo
     CDS             1..123
                     /locus_tag="AC162G_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(283..1020)
                     /gene="istB"
                     /locus_tag="AC162G_12065"
     CDS             complement(283..1020)
                     /gene="istB"
                     /locus_tag="AC162G_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HIR5076600.1"
                     /translation="MLIQPVLEQLTTLGLSGFRAALEAQQHNPQYAELTFEERLGLLL
                     DVECTRRADSRLRRRLQAAHFALPATIEDLKFSPGRGLDRRRVLDLAQAEWVPRHLNV
                     IVLGPTGAGKTFLACALGQAACRQGFSVRYERTSRLLDALHLAQADGSYAKLLASLAR
                     LPLLILDDWLRDPLTRPQARDLLEILDDRYARAATLIATQIPVADWHARIPDPTLADA
                     ILDRLVHNAHRFELKGESMRKTHSPLT"
     gene            complement(1031..2551)
                     /gene="istA"
                     /locus_tag="AC162G_12070"
     CDS             complement(1031..2551)
                     /gene="istA"
                     /locus_tag="AC162G_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032685482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HIR5076601.1"
                     /translation="MRNIHEVLRLKWESRLSHRAIANSCGISPGTVSDYVQRAQAAGL
                     SWPLPVDLTEEALYERLFLPAAGAAGRTIPQPNWAQVHTELRRQGVTLRLLWVEYREV
                     HPDGYGYSQFCEHYRQWIGHLQPTMRVCHAAGEKLFVDYAGQTVPLVNPDTGEIQSAQ
                     IFVAVLGASSYTYVEAHLHQDLPSWIGAHVRALSFFGGVPEILVPDNLKAGVKSPCRY
                     EPDLNPTYHDLAQHYGVAVLPARVRKPKDKAKVEVGVQVVERWILARLRDSTFFDVAD
                     LNRALRSLQDELNDRPMRHLGQSRRQLFEALDRPALKPLPAQPYALATWKKARVHIDY
                     HVEFEKHYYSVPHTLIHKEVDVRATEHTVEIFYRQRRQAVHPRSAAAGRFTTQAEHMP
                     PAHRAVSEWSPQRFQGWAEQIGPHTAQLISAVLASRVHPQQAYRTCLGILGLAKRYTA
                     ARLEAACRYALPAGIHSYKGLHHILEAQLDRLTPAEPPPAIRATEHANLRGPAYYQ"
BASE COUNT          457 a          820 c          984 g          481 t
ORIGIN      
        1 ggatcggcat tcctgtacgg tatttcctcc ggtgcggtac ttgccctcga ggcggccaac
       61 aagctcccca ccaaggtcaa gaagctggcg ctgtacgagc caccgttcat cgttgcgcat
      121 tccggcgaaa acgatcagtc attccggctg atggcgatcg cccattccgg tcgatggcga
      181 tcaccgaatc cggagcaagg cgatcgtgct ggctggcagc gggctgaccg cggcacagag
      241 cgaagcgacg cggatgtcga catgatacat gcactcgcgt ggtcaagtca aggggctgtg
      301 tgttttgcgc atggactctc ctttcagttc gaagcgatgg gcattgtgca ccagacggtc
      361 caggatcgcg tcggccaggg tggggtctgg gatgcgggca tgccagtcgg ccacggggat
      421 ctgagtggcg atcagggtgg ccgcgcgcgc gtagcggtca tcgaggatct ccaacaggtc
      481 gcgggcctgg ggtcgcgtca acgggtcgcg cagccagtca tcgaggatca gcaaaggcag
      541 gcgcgccagg ctggccagta gtttggcata cgagccatcg gcctgcgcca gatgcagagc
      601 gtccagcagg cgcgaggtgc gctcgtagcg cacgctgaag ccctgacgac aggcggcctg
      661 gccaagcgcg caggccaaga aggtcttccc ggcgccggtc gggcccagca caatcacatt
      721 caagtggcgc gggacccact cggcctgagc caggtcgagc acgcggcgac ggtcgaggcc
      781 acgaccgggg gagaacttga ggtcctcgat cgtcgccggc agggcgaagt gcgcggcctg
      841 caggcggcga cgcagacggc tgtcggcgcg ccgcgtgcac tcgacatcca gcagtaagcc
      901 gaggcgctcc tcgaaggtca actcggcata ctgcgggttg tgctgctggg cttcgagcgc
      961 ggcgcgaaaa ccggacaggc ctaaggtggt cagttgctcc aacacaggtt gaatcagcat
     1021 gggggtctcc tcactggtag tacgccgggc cgcgcagatt ggcgtgctcg gtggcgcgga
     1081 tggccggcgg tggttcggca ggtgtcagtc ggtcgagctg ggcctccagg atatggtgca
     1141 ggcctttgta ggagtgaatg cccgccggca gcgcgtagcg gcaggcggcc tcgaggcgcg
     1201 cggcggtgta gcgtttggcc agccccaaga tgcccaggca agtccggtag gcttgctggg
     1261 ggtgcacgcg tgaggccagc acggcgctga tcagttgggc ggtgtggggg ccaatctgtt
     1321 cggcccaccc ttggaagcgc tggggcgacc actcgctgac ggcgcggtgg gcgggtggca
     1381 tgtgctcggc ttgcgtcgtg aaacgcccgg cggcggccga acgcggatgg acggcttgcc
     1441 ggcgttggcg atagaagatt tcgacggtat gttcggtggc gcgcacgtcg acttctttgt
     1501 ggatgagcgt gtggggcacg ctgtagtagt gtttctcgaa ctcgacatgg taatcgatgt
     1561 gcacgcgcgc cttcttccag gtcgccagtg cgtagggctg cgcgggcagc ggcttcaagg
     1621 cgggtcggtc tagcgcctcg aacagttggc ggcgcgactg acccagatgg cgcatcgggc
     1681 ggtcgttgag ctcgtcttgc aggctgcgca gggcgcggtt caagtcggct acgtcgaaga
     1741 aggtggagtc gcgcaggcgg gccaggatcc agcgctcgac cacctggacg ccgacttcca
     1801 ccttggcctt gtccttgggc ttgcgcaccc gggcgggcag gacggccacg ccatagtgct
     1861 gcgccaggtc atggtaggtg ggattcaggt caggttcata gcggcaggga ctcttgaccc
     1921 cggctttgag attatccggg acgaggatct cgggcacccc gccgaagaag gacaacgcgc
     1981 gcacgtgggc gccgatccaa ctgggcaggt cttgatgcag atgggcttcg acatacgtgt
     2041 aactgctggc gcccagcacg gccacgaaga tctgagcgga ctggatttca ccggtgtcgg
     2101 ggttgacgag gggcaccgtc tgaccggcgt aatcgacgaa cagcttctcg ccggccgcat
     2161 ggcacacccg catggtcggc tgcagatgcc cgatccattg gcggtagtgc tcacaaaact
     2221 ggctgtagcc gtagccgtcg gggtggactt cgcggtattc gacccacagc aggcgcagcg
     2281 tcacgccctg acgccgcagt tcggtgtgca cctgcgccca gttcggctgc gggatcgtcc
     2341 gccccgcggc gccggcggcg gggagaaaca ggcgctcgta cagcgcctcc tctgtcaggt
     2401 ccaccggcag cggccagctc agccccgccg cctgagcccg ctgcacgtag tcactgacgg
     2461 tcccgggcga gataccgcaa ctgttggcga tggcgcggtg ggagagtcgg ctctcccact
     2521 tcagccgcag gacttcgtga atgttgcgca tggataacct ttcctggcac atgtcgacct
     2581 cctcctctgg ggtggaggac gatcatgccc gaaggcagtg agattatcca gcgccgttcg
     2641 ctctgtgccg tcggaggcgg cccacgctct ggcgatcggc atgcccggat tcagtgatcg
     2701 gcatgcgccg gaatcggtga tcggcatgca ccggattacg ca
//
LOCUS       DBNHXB010000301         9022 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2428021, whole genome shotgun sequence.
ACCESSION   DBNHXB010000301 DBNHXB010000000
VERSION     DBNHXB010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9022)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9022)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9022
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2428021"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="AC162G_12075"
     CDS             complement(<1..356)
                     /locus_tag="AC162G_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017688548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR5076602.1"
                     /translation="MYNHAPADYVCPFCALVADMENEHLLSRRNDIVLKSALTTAFIG
                     LRQWPNNPGGVIVIPNEHFENIYDLPAHLAAKIHEAAQAIAIAMKAAFVCDGVSTRQH
                     NEPAGNQDVWHYHLHVT"
     gene            complement(367..972)
                     /locus_tag="AC162G_12080"
     CDS             complement(367..972)
                     /locus_tag="AC162G_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076603.1"
                     /translation="MTRDDLETRSKSELIDLILQQESQIADLQAQLVQLQFATKPDGD
                     DRPPASLPTGRFPAAFKLILIASGIFVCAIALIIVNFKPGAATSAGRAPDFPPGSVVA
                     VQLPRPDRPDQSVPIFVVNDPVVGFLALHGRDPGSNCQMRWVPEVERIEDPCSGSKYT
                     RTGDYLSGPSPRSLDRYPVTVGENGDVAVDLSGLQRGQPRP"
     gene            complement(1045..1734)
                     /locus_tag="AC162G_12085"
     CDS             complement(1045..1734)
                     /locus_tag="AC162G_12085"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR5076604.1"
                     /translation="MSESTRIRAGKPTARKRLLPNHRPSLEHERALLREGCLRVAGLD
                     EAGRGAWAGPVVAAAVILNLAKARNLRQVHDSKQLTPRQRTDLYPLILESSLACGFGQ
                     AEASEIDTIGVVSATRLAMRRAIDVLSPPPDALIVDALRLPEVDLLQRAFNFADSISL
                     SVAAASILAKVTRDRMMVELETVYPGYGFDRHKGYGTRRHWRALEELGACPAHRATFK
                     PVAAMVGTLAR"
     gene            complement(1667..2482)
                     /locus_tag="AC162G_12090"
     CDS             complement(1667..2482)
                     /locus_tag="AC162G_12090"
                     /EC_number="2.4.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004581 - dolichyl-phosphate
                     beta-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HIR5076605.1"
                     /translation="MKCRPLRGVTISTQPFLSIVIPAHNEEHRLPPSLEKIAAFLARQ
                     SYTSEVIVVENGSVDRTADVAQDFAATHPGVTLIQEEQRGKGIAVRHGMLAARGEYRF
                     ICDADLSMPIEEVNKFLPPALDGYDVAIGSREAPGARRYNEPLYRHLMGRLFTAIVKL
                     LAVRGFEDTQCGFKCFRADVARDLFSVQRLNGMSFDVEALFIAQRRGYKIVEVPINWY
                     FSAESRVRLIDDSLRMVGELLEVRRNWRRGAYERVDANSSRKTDGPQAAPTES"
     gene            complement(2589..3698)
                     /locus_tag="AC162G_12095"
     CDS             complement(2589..3698)
                     /locus_tag="AC162G_12095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5076606.1"
                     /translation="MSNSDVVRVEELELELGSRDSDHGEASGAPEDHVLHTLTLNSNS
                     NSVLSIVIPNWNGAHHLPVCLDSLRQQTYPHIEVILVDNASSDGSLELLARDYSEVRV
                     VALTENRGFAGACNAGIRASRGEFVALLNNDTEADERWAQAVVDAFARHPEAGLIASK
                     MLLFDRRDTFHTAGDTYRLDGLPGNRGVWQRDEGQFDTEEYVFSACGGSAAYRRSMLD
                     QIGLLDEDFFFSCEDLDLAWRAQLAGWKCVYAPSAVVYHKLSSTGGGPIASYHDGRNF
                     VWLIAKNYPSSLWRQYRDAILRAQWRLAWEALRAWRGAEARARLRGMLAGLLGLPRIL
                     KKRQGVQRLRRASDDELLGVLTPIDNQHSDAQRSG"
     gene            complement(3685..4524)
                     /gene="rfbD"
                     /locus_tag="AC162G_12100"
     CDS             complement(3685..4524)
                     /gene="rfbD"
                     /locus_tag="AC162G_12100"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR5076607.1"
                     /translation="MRIYITGNKGQLGRALESALADHTLHGGDLPEVDITDLASIRPS
                     IRDARPDVVIHSAAMTDVDGCARDPDLAYRVNALGTRNVAIACQDAGCVMLAVSTNEV
                     FDGKAGRPYLEFDPTNPINPYAQSKLAAEVFVRDLLTRFYIVRTAWLFGYGGVNFVTK
                     IIDRARRDGRLRVVVDEISSPTFAADLAGAVAHLIATGAYGIYHFTNEGICSRFELAQ
                     KILELAGLAHIPIEPITLADITRPSTPPPYTPIRNFCGAQIGITLRPWQDALAEYIAH
                     EQF"
     gene            4684..5442
                     /gene="trmD"
                     /locus_tag="AC162G_12105"
     CDS             4684..5442
                     /gene="trmD"
                     /locus_tag="AC162G_12105"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014652169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR5076608.1"
                     /translation="MRFDIFTLFPSIFESPLQESILKRAIEAGLLDVRVHNIRDYATD
                     KHHVTDDYPYGGGGGMVMKPEPIFAAVEGVLEIGNYQSPIPVILLTPQGRLFNQEIAR
                     ELARHERLALICGRYEGVDERVREHLATDEISIGDYVLTGGELVALVIVDAVSRLLPG
                     VLGDASATAHDSHSGGLLEYPHYTRPPEFRGWTVPDVLLSGNHAEIDRWRRRQSLLRT
                     LRRRPDLLARAALTEEERRWLDQMVQSDQERSSP"
     gene            5648..6259
                     /locus_tag="AC162G_12110"
     CDS             5648..6259
                     /locus_tag="AC162G_12110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5076609.1"
                     /translation="MLNQRGDERLVARLREGDLSALGEVYDTYRVNIFQAALAITRDR
                     EASETILRDCFVALSARADSIDPESPLAPWLYRKVVELSYAWVTRHSKWWTSLEGLLD
                     RFDRLVASAHGASALPVSLREAQEGIDRAIDALPFNQRIVVILYYLGGLSLKEIAHIL
                     DCPVGTAKSRLHYGREALRQHLDAQLQAPEWLFKHDLAPSPGA"
     gene            6323..6898
                     /locus_tag="AC162G_12115"
     CDS             6323..6898
                     /locus_tag="AC162G_12115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5076610.1"
                     /translation="MARVREGELEALGVLYDKYRLQVFHAALAITRDHAVAEDILQEC
                     FLRLNAHAARIDATLPLLPWLYRVTVNLSYSYVSRDARRKVSLEGLIDHFEQFVAPAG
                     HAPEAQAERRDVESCVRHAVQALPFHQRAVVVLYYVGGLDLKEIAYILDCPVGTVKSR
                     LHSSREALRKQLASSLEPQTARTPGVAYDYV"
     gene            6885..7142
                     /locus_tag="AC162G_12120"
     CDS             6885..7142
                     /locus_tag="AC162G_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076611.1"
                     /translation="MITYESNRPPIELDSLLRRALCERVVDAEPSRRVRIDVLRAAMI
                     ARARRLDARDHAAAWPGWAYTHAQARMDIIRAQFGWARLLL"
     gene            complement(7102..7923)
                     /locus_tag="AC162G_12125"
     CDS             complement(7102..7923)
                     /locus_tag="AC162G_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5076612.1"
                     /translation="MQAKQPLIQVEDLRKLYPVEAGLFHKTDSWIHAVDRVRFEIERG
                     ESLALVGESGRGKTATGKLLVKLVEPTSGSIFLQANGRRIDVAGLRGKELKAYRREVQ
                     MVFQDPYESMNPRRTIYDTVAEPLAVQHVGSFAEMLSLVRLTPPASFLFRFPHELSGG
                     QRQRVAIARALIVQPSFVVADEPTSMLDVSIRAGVMRLMGDLARRQPAHRSAAALPVL
                     RALPDRREGVRGERSPAAGRQRRRTRRGMLPGLKVHSVPDGAVTATSAPNRTVRG"
     gene            complement(7956..8174)
                     /locus_tag="AC162G_12130"
     CDS             complement(7956..8174)
                     /locus_tag="AC162G_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076613.1"
                     /translation="MCEDSLLKSMRRFQHQLGMAMIYISHDMAVIAEVSDRVGVMYAG
                     RLVELGTTDLPQRHRGAESPDGWIFLSL"
     gene            8329..8511
                     /locus_tag="AC162G_12135"
     CDS             8329..8511
                     /locus_tag="AC162G_12135"
                     /inference="COORDINATES: protein motif:HMM:NF016432.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR5076614.1"
                     /translation="MLILYYLGGLRLKEIAHVLDCPIGMVKWRLHYGRELLRVWLAGE
                     AAPRARSRLEAVHDPA"
     gene            8498..8791
                     /locus_tag="AC162G_12140"
     CDS             8498..8791
                     /locus_tag="AC162G_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076615.1"
                     /translation="MTRLRRGEDGFDRAIVQTLRAGLCGEEPSPRVRDALLRGAIERM
                     HAIRDAEGTLARWRQAHPHPRLWREGNLYHAQSVPPDSARLTLETRRVMLALV"
     gene            8820..>9022
                     /locus_tag="AC162G_12145"
     CDS             8820..>9022
                     /locus_tag="AC162G_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076616.1"
                     /translation="MSSNRSLLASIDARYASADGHDPLEQENARGMMPLLEVRNLTTR
                     FKISGGFVHAVNGVTFQLEEGDSL"
BASE COUNT         1600 a         2786 c         2938 g         1698 t
ORIGIN      
        1 gtgacgtgaa gatgatagtg ccagacatcc tggttgccgg caggctcgtt gtgctgtcga
       61 gtggacacgc cgtcgcacac gaaagccgct ttcatcgcga tagcaatggc ctgagcggct
      121 tcgtgaatct ttgcggcgag gtgggccggc aggtcataaa tgttctcgaa gtgctcgttg
      181 gggatcacga tgacgccgcc cgggttgttc ggccattgcc gcaagccgat gaatgctgtt
      241 gtgagggcgc tcttgagcac gatgtcgttg cggcgcgaga gcaagtgctc gttttccatg
      301 tccgcgacga gcgcgcagaa cgggcagacg tagtcggcag gggcgtgatt gtacataagg
      361 cgctcactat ggtcggggct gcccccgttg gaggccgctc agatcgacgg cgacgtctcc
      421 gttctcgccg accgtgaccg gatatcgatc gaggctgcgc ggcgacgggc ctgagaggta
      481 atctccggtg cgcgtgtatt tcgacccgct gcacggatcc tcgatgcgct cgacttccgg
      541 tacccatctc atctggcagt tcgatccggg atcgcggccg tgcagcgcca gaaagccaac
      601 aactggatca ttcacaacga agatgggcac ggactgatcc ggcctgtccg gtcgaggcaa
      661 ttgcacggcg accacggaac ccggcgggaa atccggcgcc cggccggcgc tcgtcgccgc
      721 gcccggcttg aaattgacga tgatcagcgc aatcgcgcag acaaagattc cagaggcgat
      781 caagatcagc ttgaacgcgg cgggaaatcg tcccgttggt agcgaggccg gcgggcgatc
      841 gtcgccgtcc ggcttcgtcg cgaattggag ctgcacgagc tgcgcctgca ggtcggcgat
      901 ttgcgattct tgctggagga tgaggtcgat cagctcggat ttcgagcggg tttccaggtc
      961 gtctcgagtc atagcggtat ccgtggtggt tcgtagtgac gactaaggtc gctactacga
     1021 atcagttccg tgatcgaccg agtttcatct agcaagcgtc ccaaccatag cggccaccgg
     1081 cttaaatgtc gctcgatgcg cggggcacgc gccgagttct tccagcgccc gccaatgcct
     1141 tcgtgtccca taacccttgt gccgatcgaa accgtaccca ggatagaccg tttccagctc
     1201 gaccatcatt cgatcccgcg tcaccttcgc caagatcgac gcggccgcga cggacagcga
     1261 gatcgaatcg gcgaagttga aggcgcgctg gagcaggtcg acctccggca ggcgcagcgc
     1321 gtcgacaatc agcgcgtcgg gcggcggcga cagcacgtcg atcgcgcggc gcatcgccag
     1381 tcgcgtagcg gagacgacgc cgatggtatc gatctcggag gcttcggcct ggccgaagcc
     1441 gcatgcgaga ctcgattcga ggatcagcgg gtacaagtcg gtgcgctgcc ggggggtaag
     1501 ctgctttgag tcgtgaacct ggcgaaggtt tcgagccttc gcaagattca aaatcacggc
     1561 ggcggcgacg accgggccgg cccacgcgcc gcgccccgct tcgtcaaggc cggcgacgcg
     1621 caggcagcct tcgcgcagca gcgcgcgctc gtgctcgagc gacggcctat gattcggtag
     1681 gagccgcttg cgggccgtcg gttttccggc tcgaattcgc gtcgactcgc tcatatgcgc
     1741 ctcgccgcca gttgcgccgc acttccagca gctcgcccac catgcgcagc gagtcgtcga
     1801 tcaatcgtac gcggctctcg gcagagaagt accagttgat cggcacctcg acgatcttgt
     1861 agccgcgtcg ctgcgcgatg aacagcgcct ccacgtcgaa gctcatgcca ttcaggcgct
     1921 gcacgctgaa caggtcgcgc gcgacgtcgg cgcggaagca cttgaagccg cactgcgtgt
     1981 cttcgaagcc gcgcacggcc agcagcttca cgatggcggt gaagaggcgg cccatcaggt
     2041 ggcggtagag cggctcgttg tagcgccgcg cgcccggcgc ttcgcgcgag ccgatggcga
     2101 cgtcataacc gtcgagcgcg gggggcagga acttattcac ctcctcgatc ggcatcgaca
     2161 ggtcggcgtc gcagatgaag cgatactcgc cgcgcgccgc gagcatgcca tgccgcacgg
     2221 cgataccctt gccacgctgt tcttcctgga tcagcgtcac gccaggatgc gtcgctgcga
     2281 agtcctgcgc cacgtcggcc gtgcgatcga cgctgccgtt ctcgacgacg atcacctccg
     2341 aggtgtacga ctgtcgcgcc aggaaagccg cgatcttctc caggctcggc ggcaggcggt
     2401 gctcttcgtt gtgggcagga atgacgatgg acagaaaggg ctgggtgctg atggtgacgc
     2461 ctcgaagtgg ccgacacttc accccctctg cttcgctaca gggggctttg gaataccggt
     2521 cactttcata gcataccgcg agagccatta tccgtcaacg ttaatgggga tataggcctg
     2581 ccaggtcttc atccgctccg ctgggcgtcg gaatgttggt tatcgatcgg cgtcagaacg
     2641 cccagcagct cgtcatcgct cgctcggcgc agacgctgca ccccctggcg ctttttcagt
     2701 attctcggca acccgagcag cccagccaac atgccacgca gccgcgcccg cgcttcggcg
     2761 ccgcgccagg ctcggagcgc ttcccatgcc agccgccact gcgcgcggag gatcgcgtcc
     2821 cggtattgcc gccacaggct cgacgggtag ttcttggcga tcagccacac gaagttgcgc
     2881 ccgtcgtggt aagacgcaat tgggccgcca ccggtcgaac tgagtttatg gtagacgacc
     2941 gccgacgggg cgtagacaca tttccacccg gcgagctgcg cgcgccatgc caggtcgagg
     3001 tcctcgcacg aaaagaagaa atcttcgtcg agcaggccga tctgatcgag catcgatcgt
     3061 ctgtatgcgg cgctgccgcc gcacgcgctg aagacgtatt cctcagtgtc gaactggcct
     3121 tcgtccctct gccacacacc gcgattgccc ggaaggccgt cgagccggta agtgtcgccc
     3181 gccgtgtgga aggtgtcacg gcggtcgaag aggagcatct tggaagcgat cagcccggct
     3241 tcgggatgcc gcgcgaacgc gtcgaccacc gcctgcgccc agcgctcatc ggcttcggtg
     3301 tcgttgttga gtagcgcgac gaactcgccg cgtgaggcgc ggatgccggc gttgcacgcg
     3361 ccggcgaagc cacgattctc cgtgagcgcg acgacgcgca cctcagaata gtcgcgcgcc
     3421 agtagttcga gcgagccatc gctcgacgcg ttgtcgacca gaatcacttc aatgtgaggg
     3481 tatgtttgct ggcgcagcga gtcgaggcag accggcaggt gatgcgcgcc gttccagttg
     3541 gggatgacga tggagagtac agagttagag ttagagttta gagttagagt atggaggacg
     3601 tggtcttctg gagcgccgga tgcctcgcca tgatctgaat cccgacttcc caactctaac
     3661 tctaactcct caactctaac tacatcagaa ttgctcatgg gcgatgtact cggccaacgc
     3721 gtcctgccag ggccgcaagg tgatgccgat ttgcgcgccg cagaagttgc ggatgggtgt
     3781 gtaggggggc ggcgtcgagg ggcgcgtgat gtctgcgagc gtgatcggct cgatggggat
     3841 gtgcgccaac ccggcgagct cgaggatctt ctgcgcgagc tcgaagcgcg agcaaatacc
     3901 ctcgttggtg aagtgataga tcccgtaagc gccggtcgcg atcaggtgcg cgacggcgcc
     3961 ggccaggtcg gccgcgaagg tgggcgagct gatttcgtcc accaccacgc gcagccggcc
     4021 atcgcgcctg gcgcgatcga tgatcttggt gacgaagttg acccccccgt agccgaacag
     4081 ccaggcggtg cgcacgatgt agaagcgtgt gagcaggtcg cgcacgaaaa cctcggcggc
     4141 gagcttggat tgggcgtagg ggttgatcgg gttggtcgga tcaaactcca ggtagggccg
     4201 gccggccttg ccgtcgaaca cttcgttcgt gctgacggca agcatcacgc agccagcgtc
     4261 ctggcaggcg atcgccacgt tgcgcgtgcc gagcgcgttg acgcggtaag ccaggtcagg
     4321 gtctcgggcg cagccgtcga cgtcggtcat cgccgcgctg tggatcacga cgtcgggcct
     4381 ggcgtcgcga atgctggggc gaatcgacgc caggtcggtg atgtcgacct cgggtagatc
     4441 gccgccgtgc agtgtgtgat cggccagcgc cgattcgagc gcgcgcccga gttgcccctt
     4501 attacccgtg atgtagatgc gcatggcgcg attataccag tgagtcggtt atcagtaatc
     4561 agttgtcagt aatcagtcgt cagcgatcgg ttacgtggcg atgcgggtga ctgatcactg
     4621 attaccgatc actgattact gactactgat tcaccgatca ctgattcgtg ctacaatcac
     4681 tttatgcgct tcgacatctt cacgctcttt ccctccatct ttgagagccc gctgcaagag
     4741 agtatcctca agcgcgcgat tgaggccggc ctgcttgacg tgcgcgtgca caacatccgc
     4801 gattacgcga ccgacaagca tcacgtgacc gacgattatc cctacggcgg tggcggcggg
     4861 atggtgatga agccggaacc gatctttgcg gcagtcgagg gtgtattgga gattggtaat
     4921 taccaatcac caattcctgt aattctcctc actccgcagg gccgactctt taaccaggag
     4981 attgcccggg agctggcgag gcatgaacgc ctggcgttga tctgcggccg ttacgaaggc
     5041 gtcgacgagc gcgtacgcga gcatctggca accgacgaga tctcgatcgg cgactacgtg
     5101 ctgaccggcg gcgagctggt cgcgctggtg atcgtcgacg cggtgtcgcg gctgctcccc
     5161 ggcgtgctgg gcgatgccag cgccacggca cacgactcgc actccggggg actcctggag
     5221 tatccgcatt acacgcgccc gcccgagttt cgcggctgga ctgttccaga cgtgcttctc
     5281 tctggcaatc acgccgaaat cgaccgctgg cggcggcggc aatcactgct gcgcacgctc
     5341 cggcgacggc ctgacctgct ggcgcgtgcc gcgttaacgg aggaagagcg gcgctggctg
     5401 gatcaaatgg ttcaaagcga tcaggagcga tcctctccct gattcgttct tcacaaagtc
     5461 tcgcgtcatt ctcccgattt cccgctctct ttgaaccttt ccgcttgcct gggagtgata
     5521 tttccagatc ataggcgtat cggctcagaa tctgggccag cctgctgaat gagctggcgc
     5581 gcgcattttt tgacgtggga agcggagact gctccaggac accggaggct gttcaagagg
     5641 gggagcgatg ctcaatcaac gcggcgacga acgattggtt gcccgcctac gggaaggcga
     5701 tttgtccgcg ctgggcgaag tgtacgacac ataccgcgtg aacatctttc aagcggcgct
     5761 ggctatcacg cgcgatcgcg aggcgtcgga gactattctg cgcgactgct tcgtcgcgct
     5821 gagcgcgcgc gccgactcta tcgatcccga atctccactc gctccctggc tgtatcgcaa
     5881 agtcgtcgag ctgagttatg cgtgggtgac gcgtcactct aaatggtgga cgtctctgga
     5941 ggggctgctc gaccgattcg accggctcgt cgcatcggcc catggcgcgt cggcgctgcc
     6001 cgtgagcctg agagaggccc aggagggcat cgaccgcgcg atcgacgccc tgccgttcaa
     6061 ccagcggatt gtagtgatcc tgtactatct gggcgggctg agcctcaagg aaatcgcgca
     6121 catcctggat tgtccggtgg gcacagccaa gtcgcgcctg cactatgggc gcgaagcgct
     6181 gcgccagcat ctcgacgcgc agcttcaggc gcctgagtgg ctcttcaagc atgatctagc
     6241 gccaagcccc ggcgcgtaat ccgagccgaa gtggggaggg agaggtatgg cgccagcagg
     6301 gcaggaggag gatcgacact tgatggcccg cgtcagggag ggcgaattgg aggcgctggg
     6361 cgtgctgtac gacaagtatc gccttcaggt ttttcacgcg gcgctggcca tcacgcgcga
     6421 ccacgccgtc gcagaggaca ttctgcagga gtgcttcctg cgcctgaacg cgcacgccgc
     6481 gcgcatcgac gcgacgctgc cgttgctgcc gtggttgtat cgggtgacgg tcaatttgag
     6541 ctactcgtac gtgagccgcg acgcgcgccg gaaggtgtcg ctggagggat tgatcgatca
     6601 cttcgagcag ttcgtcgcgc cggccgggca cgcgccggaa gcacaggccg agcgccgcga
     6661 cgtggagtcc tgcgtgcggc acgcggttca ggcactgccc tttcatcagc gcgccgtcgt
     6721 ggtgttgtac tatgtcggcg gtctcgatct gaaagagatc gcgtatattc tcgattgccc
     6781 agtcggcaca gtcaaatctc gcctgcacag cagtcgcgag gcactgcgca agcagctcgc
     6841 gagcagcctg gagcctcaga cggcgcgcac gccgggagtc gcgtatgatt acgtatgagt
     6901 cgaatcgacc accgatcgag ctggacagcc tgctgaggcg cgccctgtgc gagcgggtag
     6961 tcgacgccga gccgtcgcgc cgggtgcgca tcgatgtgct gcgcgccgcg atgatcgcgc
     7021 gggcgcggag actggatgcc cgcgaccacg ccgctgcctg gccaggctgg gcctacaccc
     7081 acgcgcaggc gcgaatggac attatccgcg cacagttcgg ttgggcgcgc ttgttgctgt
     7141 gacggcgccg tccggcacgc tatgcacttt cagacccggt aacatgccac ggcgtgtccg
     7201 ccgtcgttgt cttccagcgg cgggtgatcg ttctcctcgc acaccttcgc ggcgatcggg
     7261 cagcgctcgt aaaaccggca gcgcggcggc ggatcgatgg gccggctgac gccgcgccag
     7321 atctcccatc aggcgcatca cgcccgcccg gatcgacacg tcgagcatgg acgtcggctc
     7381 gtccgcgacg acgaaactgg gctggacgat gagcgcgcga gcgatcgcca cgcgctggcg
     7441 ctgcccgccg gacagctcgt gcggaaagcg gaacaggaag ctggccggcg gcgtcagtct
     7501 gaccaggctc agcatctcgg cgaagctgcc cacgtgctgc acggcgagcg gctccgccac
     7561 ggtgtcgtag atggtgcggc gcgggttcat cgactcgtag ggatcctgga acaccatctg
     7621 cacctcgcgc cggtacgcct ttagctcctt gccgcgcagc ccggccacgt cgatacgccg
     7681 tccgttcgcc tgcaggaaga tcgatcccga cgtcggctcg accagcttca cgagcagctt
     7741 gccggtggcg gtcttgccgc ggcccgactc gccgaccagt gccaggctct cgccccgctc
     7801 gatttcgaac ctgacgcggt cgacggcgtg aatccacgag tcggtcttgt ggaacagccc
     7861 cgcttcgacg ggatataact tcctcaggtc ctccacttga attaagggtt gctttgcctg
     7921 catgcgggcc tcgaatgaac ggctctgccg cagcatcaca gagacagaaa gatccagccg
     7981 tccggactct ctgcgcctct gtgtctttgt ggcagatcgg tcgtccccaa ctcgaccagc
     8041 ctgccagcat acatgacgcc gacgcgatcg ctcacctcgg cgatcaccgc catgtcgtgc
     8101 gagatataaa tcatcgccat gcccaattga tgctggaacc ggcgcatgga cttgagcagg
     8161 ctgtcctcgc acattgagtc ttaaaaggtt caacccaaac gtgccaacgt gcgacgcact
     8221 tcaagaatgc gtcgcacgtt gacctgtgcc ctgaaccttt cgggtcgtgc gtgagtgttt
     8281 tgatcgtcgg agtggagtat gcgatatggc gacgaatcaa gatgcgcagt gctgattctg
     8341 tattaccttg gcggtcttcg tctgaaggag attgcgcacg tgctggactg cccgatcggc
     8401 atggtgaagt ggcgcctgca ctatggccgc gagctgctgc gcgtgtggct ggcgggcgag
     8461 gctgcgccgc gtgcacggtc ccggctggag gccgttcatg acccggctta ggcgcggcga
     8521 agacggattc gatcgagcga tcgtccagac gctgcgcgcc ggcctgtgcg gcgaagaacc
     8581 atccccgcgc gtccgcgacg cactgctgcg cggagcgata gagcgaatgc acgcgatacg
     8641 ggacgcggag ggcacgttgg ctcgctggcg ccaggctcac cctcacccga ggttgtggcg
     8701 cgaaggcaat ctgtatcacg cgcagagtgt gccgcccgac tcggcacggc tcacgctcga
     8761 aacacgccgc gtcatgctgg cactcgtctg actcgctcgc gcattccctg tgatttgcca
     8821 tgagctcgaa tcgatccctg ctcgcgtcga tcgatgcgcg ttacgcaagc gctgacgggc
     8881 acgacccgct cgagcaggag aatgcgcggg gcatgatgcc tttgctcgaa gtcaggaacc
     8941 tcacgacgcg attcaagatc tcgggcggct tcgtgcatgc cgtcaacggc gtcacgttcc
     9001 aattggagga gggcgactcg ct
//
LOCUS       DBNHXB010000302        10164 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_582175, whole genome shotgun sequence.
ACCESSION   DBNHXB010000302 DBNHXB010000000
VERSION     DBNHXB010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10164)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10164)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10164
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_582175"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="AC162G_12150"
     CDS             complement(<1..829)
                     /locus_tag="AC162G_12150"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR5076617.1"
                     /translation="MKHFVLKLSMALTLGAGLAIVLLWSLGRRSTELPIAHAGELRVC
                     PVGCTYSSIQAAVDAANTGDIVKVAVGTYTGVSARAGVTQVVYISATITIQGGYTTTN
                     WTTPYPITQPTTLDAQGQGGVMYITGNVSPTVEGLHITGGASFFDFNQDLYLGGGMYV
                     VSATPTISGNWVFSNTLGVGPCLCNGGGGLFLWHSKATVRGNRVYSNTSMLGGGLMLS
                     ESDATLSGNWVFGNISSRRGGGLALVRSAAAIIGNSIFSNSASTNGGGIHVLDSAATI
                     "
     gene            1286..2155
                     /gene="rpsB"
                     /locus_tag="AC162G_12155"
     CDS             1286..2155
                     /gene="rpsB"
                     /locus_tag="AC162G_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR5076618.1"
                     /translation="MAVISMKALLETGVHFGHRTRRWNPKMKPFIFTERNGIHIIDLQ
                     QTIQALDQAHHTMRDTFANGGTVLFVGTKPQAQEVVATECGRAGVPYVAQRWLGGTLT
                     NWRTIRQRIDTLINLEARRDRGDFELLPKKEALQLNRQIDKLNARLGGIKTMRALPNA
                     LFVIDVRREDTAIREANALDIPVIAMVDTNCDPDPIDLIIPSNDDAIRAIKLIVGRMA
                     DSALEGMGMRKDMPGAEAEAMEEADERYLGEATLAKLRAGELAFEEEGEETLHGGDDE
                     VPDALPPGDEGEA"
     gene            2168..2770
                     /gene="tsf"
                     /locus_tag="AC162G_12160"
     CDS             2168..2770
                     /gene="tsf"
                     /locus_tag="AC162G_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR5076619.1"
                     /translation="MCVDITAEQIKKLREATGAGVLDSREALRQTGGDFDKAAAYLRE
                     KGLIKAAKKSDRAASDGVIGSYIHYNNRVAVLVELNSETDFVARTEDFGALAQSLAMH
                     IAMANPLYIAPEDVPADVLEAKRAIYRQEAETTGKPAPVVDKIVDGKIEKFYDEACLL
                     RQPYIKDDKVRIGDMIAQAIARLGENIMVRRFARFEVGGE"
     gene            2823..3539
                     /gene="pyrH"
                     /locus_tag="AC162G_12165"
     CDS             2823..3539
                     /gene="pyrH"
                     /locus_tag="AC162G_12165"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR5076620.1"
                     /translation="MPVPKYKRILLKLGGEALAGKGGFGIDPDKAEEVAAKIKAIRDI
                     DVQVAIVLGAGNLWRGKDGLAHGMERATADHMGMIATVMNALALQDALERIGLITRVQ
                     TAITMQSIAEPYIRLRAIRHLEKDRVVIIGGGTGNPYFTTDTAAALRAMEIGCDVLIK
                     ATKVDAVYHEDPRINPQAKRFESLSYIEALNLRVGVMDSTAISLCMDNAMPILVLDLW
                     QPHGLERAVMGEPIGTLIHG"
     gene            3621..4178
                     /gene="frr"
                     /locus_tag="AC162G_12170"
     CDS             3621..4178
                     /gene="frr"
                     /locus_tag="AC162G_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR5076621.1"
                     /translation="MVKDILKAAEDHMKKTVEVLEQDLKTIRTGRASPALVERLPVNY
                     YGAPTPLEQLATISAPEPQMLAIRPYDPGSLKEIERAILASDLGLTPNNDGKIIRLPI
                     PRLTEERRHELVKVVHKRIEEARVAARNVRREAQDQMREAQKEKLISEDDLKRGRDDL
                     QKLTDKIMEQIDAVGERKEQEIREV"
     gene            4246..4977
                     /locus_tag="AC162G_12175"
     CDS             4246..4977
                     /locus_tag="AC162G_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR5076622.1"
                     /translation="MVESAPLPAVPYHLAIIMDGNGRWAQARRLPRLAGHRAGTENLR
                     RVLRACKDFGVKILTVYAFSTENWRRPAEEVSGLMTILDEYIVREMRALHEEGVQIRH
                     LGRMDGVRPGLQEKIKQAIELTRNNTTLILNVAFNYGGRQEILDAVRAILADGVAPED
                     VDEALFSRYLYTAGQPDPDLIIRTSGEARISNFLLWQGAYAELYSTPVFWPDFDKHEL
                     RKALDHYANRERRFGLTTAQLATVR"
     gene            5080..5874
                     /locus_tag="AC162G_12180"
     CDS             5080..5874
                     /locus_tag="AC162G_12180"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR5076623.1"
                     /translation="MLLTRVLSAIVLVPLVVAAVLIGDWVLVALLAAVLGLASGEFVR
                     LMQQGGYRPVWLVAYVLVATLLLDSTVIEARLMITVVSLTLIGSLVWHMGHRTATVTA
                     DWALSVAMGLYLGWAGRQSVAIRAFDDGAAWLVLVLTGTWLADSGAYFVGSRAGHHKM
                     APTLSPKKSWEGLAGGIVAGVAGNGLLAAVFGLPPIHGAALGLLGATIGTLGDLSISM
                     MKRQVGAKDSSQLIPGHGGALDRVDSLLFSVVAGYLYLTWFTGMPT"
     gene            5961..6956
                     /gene="glpX"
                     /locus_tag="AC162G_12185"
     CDS             5961..6956
                     /gene="glpX"
                     /locus_tag="AC162G_12185"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005480703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HIR5076624.1"
                     /translation="MTASPTIDPDRNLALELVRTTEAAAMAAGRWMGRGDKNAADQAA
                     VVAMRTIFNTINMDGVVVIGEGEKDHAPMLYNSERLGSGTPPLVDIAVDPIDGTRLTS
                     LGLPGAISVVAVAERGTMYNPQHIVYMNKIAVGPQAKGAVSLDAPVKDNVRRVARALD
                     KDVNDVTVVVLDRPRHETLLKEIRAAGARIKLIPDGDVSGGLLSAFEESGVDMLMGIG
                     GSPEAVITACALKCLGGEIQCKLWPRDEEERRRAMEIGLELDRVFTTDDLVRGENVFF
                     VATGITDGEWLRGVKYASKGIRTTSVVMRSRSGTVRTVDAVHRAEKLAQLTSLYE"
     gene            7251..8519
                     /gene="rho"
                     /locus_tag="AC162G_12190"
     CDS             7251..8519
                     /gene="rho"
                     /locus_tag="AC162G_12190"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR5076625.1"
                     /translation="MDFIELESKTLGELRDLARSMNLSGTSRLKKEDLILRLLRAKAE
                     GQGYIFGGGVLDIVEDGIGFLRSDHYLPGPEDVYVSQSQIRRFGLRTGDMVVGQVRPP
                     KDSEKYFGLLRVEAVNGLDPEAAKARPIFEKLTPIFPNERFNLEVESNNLTTRLLDII
                     APVGRGQRGLIVSPPKAGKTTVLKHIANAISRRYPDVHLMVVLIGERPEEVTDMDRSV
                     EAEVISSTFDEPPTSHVHVAEMALARAKRLVEGGRHVVILLDSITRLGRAYNNTVVSS
                     GRTLSGGIDPAALYPPKGFFGAARNIEEGGSLTIIATCLVDTGSRMDDVIYEEFKGTG
                     TMELHLSRRLQERRVFPAFDIERSGTRREELLLDQETLSKTWLIRRMLDQMTNSNGGM
                     SEGQAMEAFLARMARTKTNAEFLAALRDGM"
     gene            8721..9962
                     /locus_tag="AC162G_12195"
     CDS             8721..9962
                     /locus_tag="AC162G_12195"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR5076626.1"
                     /translation="MDIPDFKPRPALRYTPPAGDPDVRRSPRLPAITPDRVSQIVVGW
                     VAFFSRLALFITSDVTRTSFARYISHATILGLAIGAIVFSASAARIAQPELVPNAPLR
                     ASSAFELASVDYTLYAAGGALLYPQSDIVRQADPYTAIPDRPRRGVIEYTVQPGDVLF
                     TIAGRFGVSPESILWNNQETLNNDPHKILPGTVLLIPPVSGVIHTIQEGDTLESIAAA
                     YKVTVDAIVVEGAQWNNLLEGRLPPAGSTLIVPGGQKEFKGWELPKAARRTATGIAAP
                     ALGLCSSVSGGLVGSGSFIWPANSHWVSGYNYSAWHQGVDLAGRLGDAVFAADSGFVV
                     YAGWSNVGYGNLVVIDHANGWQTWYGHLSQIYVTCGQTVWQGGTIAAVGSTGNSSGPH
                     LHFETRFQGSLPNPWNVLPAP"
BASE COUNT         1961 a         3183 c         3164 g         1856 t
ORIGIN      
        1 tgattgtggc cgcactgtcc agcacgtgaa taccaccacc gttcgtgctg gcgctgttcg
       61 aaaagatgct gttcccgatg atggcggcgg cgctgcgtac cagggccaag ccgccccctc
      121 tgcgagagct gatattgccg aacacccagt tgccgctcag ggtcgcatca ctctcgctga
      181 gcatcaggcc gccacctaac atggacgtat tgctgtatac ccgattcccg cgaacggtag
      241 ccttgctgtg ccacaagaac aggccgcctc ctccattgca caagcaaggt cctacaccca
      301 gagtgttgct aaacacccaa ttgccgctga tggttggcgt ggctgagacg acatacattc
      361 ctccgcctaa gtaaagatcc tgattgaagt cgaaaaagga cgcgcctccc gtaatgtgta
      421 gtccttcgac tgtaggactg acattcccgg tgatgtacat cacacccccc tgcccctgcg
      481 catcgagcgt cgtcggttgg gtgatggggt agggcgtcgt ccagttggtc gtcgtgtagc
      541 cgccctggat ggtgatggtc gcactaatgt aaacgacctg cgtcacgccc gcgcgggcgc
      601 tcacgccggt gtaggtgccg actgccactt tgacgatgtc cccggtgttg gcagcgtcga
      661 cggcagcctg gatggaggag taagtgcagc cgactgggca gacgcgcagt tcgccggcgt
      721 gggcaatcgg cagctccgta ctccgtctgc ccagtgacca cagtagcaca atagccagcc
      781 cggctccaag tgtcagggcc atgctgagct tgaggacaaa gtgcttcatg gaactcctcc
      841 tgctgatctc gacgggcgtg gcctgtcgag ttcagcttgt cgttgcccgt gtggcatatg
      901 cgccgtatgc ggttgagacc gaagtgtgaa ggaaggttat ggagccgaca agaaacgcaa
      961 acaggaaccg tggagctgcc acattcgata cgtacgtagg tgtcgatatt cgatcggcaa
     1021 agatcagtat agcactggga cactggctgt caacatttcg ctttccttca caatcaggtg
     1081 gccgcatggc tcggtcaggt gcatttcact ttgaagttgt cgcttcctaa taactgtggt
     1141 acaatcttgc ccggtcgcgg cacgcgttgc gccgcgacac atcatgtacg ccctttgatc
     1201 aacgaggcgt gccggaacca ggccgttccg gctctcagcg agacgagaag ggcgggcgca
     1261 taaaacgcaa aggagacaat cgaatatggc agtcatctcg atgaaggcgc tcctggagac
     1321 aggcgtgcac ttcggtcacc gcacccggcg ctggaacccg aagatgaagc ccttcatctt
     1381 caccgaacgt aacggcatcc acatcatcga cctccagcag accatccaag ctcttgatca
     1441 agcgcaccac acgatgcgcg acacgttcgc caacggcggc acggtgttgt tcgtcggcac
     1501 caagccgcaa gcgcaggagg tcgtcgcgac cgaatgcgga cgcgccggcg tgccgtacgt
     1561 cgcgcagcgc tggctgggcg gcacgctcac caactggcgc accatccgcc agcgcatcga
     1621 taccctgatc aacctggagg cccggcgcga ccgaggcgac ttcgagctgc tccccaagaa
     1681 agaggcgctg cagctcaacc gccagatcga caagctcaac gcgcggctgg ggggcatcaa
     1741 gaccatgcgc gcgctgccga acgcgctgtt cgtgatcgac gtacgccgcg aggacacggc
     1801 tatccgcgag gccaacgcgc tagacattcc ggtgatcgcc atggtcgata ccaactgcga
     1861 tccggacccg atcgacctga tcatcccgtc caacgacgac gcgattcgcg cgatcaagct
     1921 gatcgtcggc aggatggccg actcggcgct ggagggcatg ggcatgcgca aagacatgcc
     1981 gggggccgaa gccgaagcaa tggaagaggc cgacgagcgc tatttgggcg aggccacact
     2041 ggcgaagctg cgcgccggcg agcttgcctt cgaggaagaa ggggaagaaa ccctccacgg
     2101 cggggacgac gaagtgcctg acgccctccc gccgggtgac gaaggtgaag cataacgttc
     2161 gaggagtatg tgcgtggaca tcaccgcgga gcagatcaag aaattacgcg aggcgacggg
     2221 agccggcgtg ctggacagcc gcgaggcgct ccggcaaacg ggtggcgact tcgacaaggc
     2281 tgccgcttac ctgcgcgaga agggcttgat caaggctgcc aagaagtccg accgcgccgc
     2341 gagcgacggc gtcatcggct cgtacatcca ttacaacaat cgcgtcgccg tgctcgtcga
     2401 gttgaattcg gaaaccgatt tcgtggcgcg cacggaagac ttcggcgcgc tggcgcagag
     2461 cctggcgatg cacatcgcca tggccaatcc tctctatatc gcgccggagg acgtgccggc
     2521 ggacgtgctg gaggccaagc gagccatcta tcgccaggaa gccgagacca ccggcaagcc
     2581 ggccccggtc gtcgacaaga tcgtcgacgg caagatcgag aaattctacg atgaggcctg
     2641 cctgctgcgc caaccataca tcaaggacga caaagtgcgt atcggcgaca tgatcgcgca
     2701 ggccatcgcc aggctcggcg agaacatcat ggtgcggcgt tttgcccggt tcgaagtcgg
     2761 aggcgagtga ttttcaagat gaccggcact tcccaagtgc cggtcatctt cacagaggtt
     2821 ctatgcccgt tccgaagtat aagcgcatcc tgttgaagct cggcggcgag gcgctggccg
     2881 ggaagggcgg cttcggcatc gatcccgaca aggccgagga agtcgccgcc aagatcaaag
     2941 ctatccgaga tatcgatgtg caggtcgcca tcgttttagg cgcgggcaac ctgtggcgcg
     3001 gcaaggatgg gctggcgcac ggcatggaac gcgccaccgc cgaccacatg ggcatgatcg
     3061 ccacggtcat gaacgcgctc gcgctgcagg atgcgctgga gcgcatcggc ctcatcacgc
     3121 gcgtgcagac ggcgatcacc atgcagtcga ttgccgagcc gtacatccgg ctgcgcgcca
     3181 ttcgccatct ggagaaagac cgcgtcgtga tcatcggcgg cggcacgggc aatccctact
     3241 tcaccaccga cacggcggct gccctgcgcg ccatggagat cggatgcgac gttctgatca
     3301 aggccaccaa ggtcgacgcc gtctaccacg aagacccgcg catcaacccg caggcgaaac
     3361 gcttcgagtc gctttcctac atcgaggcgc tgaatttgcg ggtgggggtg atggactcca
     3421 ccgccatttc gctgtgcatg gataatgcga tgcctatcct ggtgctcgac ctgtggcagc
     3481 cccatggcct ggagcgcgcc gtgatgggcg aacccatcgg cacgctgatc cacggctgat
     3541 cggccacggt cgcacttgcc acaaagccca agcgcgcata caatattggt actgcacccg
     3601 ccttgcgaag ggttcccgcg atggtcaaag acatcctcaa ggctgctgaa gaccatatga
     3661 aaaagacggt cgaagtgctg gagcaggacc tgaagactat tcgaacaggc cgcgcctcgc
     3721 cggcgctggt cgagcggctc ccggttaact actacggcgc gccgacgccg ctcgagcagc
     3781 tcgccacgat ttccgcgccg gagccgcaaa tgctcgccat tcggccttac gatcccggct
     3841 ccctgaaaga gatcgagcgc gccattctgg cctccgatct gggcctcacg ccgaacaacg
     3901 atggcaagat cattcgcctg cctattccgc gtctgaccga agagcggcgc catgagctgg
     3961 tcaaagtcgt gcacaagcgg atcgaagagg cgcgggtcgc cgcccgcaat gtccgccgcg
     4021 aggcgcagga tcagatgcgt gaggcgcaga aagagaagct gatctcggag gacgacctca
     4081 agcgcggccg tgacgacctg caaaaactca ccgacaagat catggagcag atcgacgcgg
     4141 tgggtgagcg caaagagcaa gaaattcgag aagtgtagcg gtgacccccc tccggctccc
     4201 cccgtcagaa cgggggagcc ggaggggggc ggcctgcaaa tcctcatggt cgaatcagca
     4261 cctctccccg ccgttcctta tcatctggcc atcatcatgg acggcaacgg ccgatgggcg
     4321 caggcccgcc gcctgccgcg cctcgccggc catcgcgccg gcaccgaaaa cctgcgccgg
     4381 gtgctgcgcg cttgcaagga tttcggcgtc aagattctga cggtctacgc attctccacg
     4441 gaaaactggc gccgccccgc cgaggaagtc agcgggctga tgacgatcct cgacgaatac
     4501 atcgttcgcg agatgcgcgc ccttcacgaa gaaggcgtgc agattcgtca cctgggccgc
     4561 atggacggcg tcaggcccgg cctgcaagag aagatcaagc aggcgatcga gctgacccgc
     4621 aacaacacga cgctgattct caatgttgca ttcaactacg gtggacgcca ggagatcctg
     4681 gacgcggtgc gcgcgattct ggccgacggc gtcgcgcccg aagatgtcga cgaggccctg
     4741 ttcagtcgat acctgtacac ggccggccag cccgatccgg atctgatcat ccgcaccagc
     4801 ggcgaggcgc gcatcagcaa tttcctgctg tggcagggcg cttacgccga gttgtactca
     4861 actcctgtat tctggcccga cttcgacaag cacgaactgc gcaaggcgct ggatcactac
     4921 gccaaccgcg agcgccgctt cgggctgacg accgcgcaac tggcaactgt gcgctgatct
     4981 tgtccgctcg tcgcatagtc gtgtggtagg gtcgtgagtc gttgatgatt ctgttgacgg
     5041 gcggactgtg cgacgaccag actggccggc tactcgatta tgttgctgac gcgcgtgctc
     5101 agtgccattg tgctcgtccc cctcgtcgtg gcggccgtcc tgatcgggga ttgggtgctc
     5161 gtcgctctgc tggccgccgt gctggggctc gcgtcgggag agtttgttcg cctgatgcag
     5221 cagggcggct accggccagt ctggctcgtc gcgtatgtcc tcgtcgccac gctgctcctc
     5281 gattcgaccg tcattgaagc gcggttgatg atcacagtcg tgagcctgac gttgatcggc
     5341 tcgttggtct ggcacatggg gcatcgcacg gcaacggtca ccgccgactg ggcgctctcc
     5401 gtcgccatgg gcctgtatct gggctgggct ggacggcagt ccgtcgcgat ccgcgccttc
     5461 gacgatggcg ccgcctggct tgttctggtc ttgacaggca cctggttggc cgatagcggc
     5521 gcttatttcg tcgggtcgcg cgccggccat cacaagatgg cgccgacgct cagcccaaag
     5581 aagagctggg agggtctcgc aggcggaatc gtcgccggcg tcgccggaaa cgggctgctg
     5641 gccgctgtgt tcgggctgcc gccgattcac ggcgcggcgc ttggtctgct gggtgcgacc
     5701 atcggaacgc tgggcgatct gtctatctcg atgatgaaga ggcaggtagg cgctaaggac
     5761 tccagtcagc ttatccccgg gcacggcggc gcgctggacc gagtcgacag cttgctgttt
     5821 tcagtcgtgg cgggttatct ctacctgacc tggtttaccg ggatgcccac ttagagactg
     5881 tctcaaaacc ctgtggttgg cgcatcccca tgcgtcaaca acttaacaga caggctctta
     5941 ccttgcgaga aggatgtgac atgactgctt cgccgacaat cgatcccgat cgcaatctgg
     6001 cgcttgagct ggtccgcacg acggaggcgg cagccatggc cgccggacgc tggatggggc
     6061 gtggggacaa gaatgctgcc gaccaggccg ccgtcgtcgc catgcgcacg attttcaaca
     6121 cgatcaacat ggacggcgtc gtcgtcatcg gcgaaggcga aaaagaccat gcgccgatgc
     6181 tgtacaatag cgagcgtctg ggttcgggca caccgcctct cgtcgacatt gccgtcgatc
     6241 cgatcgacgg cacgcgcttg acttcgctgg gcctgcccgg cgcgatctcg gtcgtcgccg
     6301 tggccgagcg cggcacgatg tacaaccccc agcacatcgt gtacatgaac aagatcgccg
     6361 tcggtccgca ggccaagggc gcggtcagcc tcgacgcgcc ggtgaaagac aacgtgcgcc
     6421 gcgtcgccag ggcgctcgac aaggacgtaa acgacgtgac cgtcgtcgtg ctcgatcgac
     6481 cgcgccatga aaccctgttg aaggagattc gcgcggccgg cgcgcgcatc aagctcatcc
     6541 ccgacggcga cgtgtccggg ggcctgctgt ccgcctttga ggagtcgggc gtcgatatgc
     6601 tcatgggcat cggcggatcg ccggaagccg tcatcacagc ttgcgccctg aaatgcctgg
     6661 gcggcgaaat ccaatgcaaa ttgtggccgc gcgacgaaga agagcgccgg cgcgcgatgg
     6721 agatcggcct ggagctcgac cgcgtgttca cgacagacga tctggtgcgt ggggaaaacg
     6781 tgttcttcgt cgccacgggc atcaccgatg gcgagtggct gcgcggcgtg aaatatgcca
     6841 gcaaaggcat ccgcaccacg tcggtcgtca tgcgcagtcg ttctggaacg gtgcgcaccg
     6901 tagacgccgt ccaccgcgcg gaaaagttgg cgcagctcac cagcttgtat gaatagttga
     6961 ctttcaagtt gctcgtgcta taattgacat gaggttccac gtttgtcgta cagcagcgac
     7021 tggactccct gataactcct tacggactta gcgattcctc ttttcgagcc tacgacatcg
     7081 aagcgggccg ggcgacctgc cggccggatg tgatgcgcag catggcaggg ccgctcaagg
     7141 gttcgttttg ccgaagcagg ttcgaaagtc tcgtcgaaag tgatcctgtc tcatctggca
     7201 ttcgatgacc tggattgaca acgttacact gactcatacg gagtgtcgcc atggatttca
     7261 tagaacttga atccaaaacg ctgggcgagc tgcgcgatct ggcgcgctcg atgaacctgt
     7321 ccggcaccag ccgcctcaag aaagaggatt tgatcctgag gctgctgcgg gccaaagccg
     7381 aggggcaggg atacatcttc ggtggcggcg tgctggacat cgtcgaggac ggcatcgggt
     7441 tcttgcgcag cgatcactac ctgcccggcc cggaggacgt ctacgtctct cagtcacaga
     7501 tccgtcgctt cggcctgcgc acaggtgata tggtcgtcgg ccaggtccgc ccacctaagg
     7561 atagcgaaaa gtacttcggg ctgcttcgcg tcgaggcggt gaatggcctc gatccggaag
     7621 ccgccaaggc ccggcctatc ttcgagaagc tcacaccaat tttccccaac gagcgcttta
     7681 acctggaagt cgagtcgaat aacctcacga cgcgcctgct cgatatcatc gcgccggtcg
     7741 ggcgcgggca gcgcggcctg atcgtctccc cgccgaaagc cggcaagacg accgtgctca
     7801 agcacatcgc caatgccatc tcacggcgct atccggacgt gcacctgatg gtcgtcctca
     7861 tcggtgagcg ccccgaggaa gtgaccgata tggatcgctc ggtcgaagcg gaggtcatca
     7921 gcagcacgtt cgacgagccg ccgacgagcc acgttcacgt tgccgaaatg gcgctggccc
     7981 gcgccaagcg cctggtcgaa ggaggccggc acgtcgtcat cctgctcgat tcgattacgc
     8041 ggctggggcg cgcctacaac aacacggtcg tgtcgagcgg gcgcacgctg tcgggcggca
     8101 tcgatccggc cgcgctctac ccgcccaagg gattcttcgg ggcggcccgc aatatcgagg
     8161 agggcggcag cctgacgatt atcgccactt gcctggtcga caccggcagc cgcatggacg
     8221 acgtcattta cgaggagttc aaaggcaccg gcaccatgga acttcacctg agccgccggc
     8281 tgcaagaacg ccgcgtcttc ccggcgttcg atatcgagcg ctccggcacc cgccgcgaag
     8341 aactcctgct cgatcaggag acgctcagca agacgtggct cattcgccgc atgctcgacc
     8401 agatgaccaa ctccaatggc ggcatgtccg aggggcaggc catggaagcg tttctcgcgc
     8461 gtatggcgcg caccaagacc aatgccgagt tcctggccgc gctccgagac ggcatgtaac
     8521 cttttgcaaa cggctttctt cgcctgacaa agccagactg tacgatatac ctacgccata
     8581 tctgggggta tttggcctag tcacccccta tatgtgggtt gtaagtagtg tgcccggact
     8641 tgtcctcggc gctctctgtg gtataatgcc aggcaattcg agggacgacg ctggccgtct
     8701 ctccttcgaa tgtccccacc atggatattc ccgactttaa gccgcgcccg gctctgcgat
     8761 acacacctcc ggcgggggac cccgacgtga ggcgcagccc gcgacttcct gccataacgc
     8821 ctgatcgggt ttcgcagatc gtggtgggat gggttgcctt cttcagccgg ttggcgctgt
     8881 tcatcacgag cgatgtcacc cgaaccagct tcgcgcgata tatctcgcat gccacgattc
     8941 tgggcttggc catcggtgcg atcgtcttca gcgccagtgc ggcccgtatt gcccagccgg
     9001 aactggttcc gaacgcgcct ttgcgcgcca gttcggcgtt cgagcttgcg tccgtcgact
     9061 acacactgta cgccgcaggc ggcgccctgc tgtatccgca atcggatatc gtgcggcagg
     9121 ccgatcccta tacggccatt cccgaccgcc cgcgccgcgg cgtgatcgaa tacacggtgc
     9181 agccaggcga cgttctgttc acgattgccg gccggttcgg cgtctcgccc gaatccatct
     9241 tgtggaacaa tcaggaaact ctgaacaacg atccccacaa gatattgccc ggcaccgtgc
     9301 ttctgattcc ccctgtcagc ggtgtgatac acaccatcca agaaggcgac acgctggaga
     9361 gcatcgccgc agcctacaag gtcaccgtcg acgccatcgt cgtcgagggc gcgcagtgga
     9421 acaacctgct cgagggtcgc ctgccgccgg ctggcagcac cttgatcgtg cccggcggtc
     9481 aaaaagagtt caaggggtgg gaactgccta aggctgcgcg ccgcactgcc acgggcattg
     9541 ccgcgcctgc gctagggttg tgctccagcg tgagtggcgg cctggtcggc agtggatcgt
     9601 tcatctggcc ggcgaacagc cattgggtca gcggctataa ctactcggcc tggcatcaag
     9661 gcgtggatct cgccggccgg ctcggcgacg ccgtcttcgc cgccgacagc ggattcgtcg
     9721 tgtatgcagg ctggagcaac gtcggctacg gcaacctggt cgtcatcgat cacgccaatg
     9781 gatggcagac gtggtacgga cacctgagcc agatctacgt gacttgcggc cagaccgtgt
     9841 ggcagggcgg cacgatcgct gcggtcgggt cgactggcaa ttcgagtggg ccgcacctcc
     9901 acttcgagac gcgcttccag ggcagtctcc cgaatccttg gaacgtcctt ccagccccgt
     9961 agcgggcagt cgccaccggc cgccatcctc tcatggatag ccattcgcac ccgctgcttg
    10021 tcaatccggc gcaagccggc catcgccgaa tgtcgtcgcc agagcgcggt ctcgttctcc
    10081 aggcctatcg tttttggtcg gctgccatca gatggtcggc tgtcacggcc gtcggaagca
    10141 tggcgctcac cggcgccggg gagc
//
LOCUS       DBNHXB010000303         6523 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1756240, whole genome shotgun sequence.
ACCESSION   DBNHXB010000303 DBNHXB010000000
VERSION     DBNHXB010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1756240"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="AC162G_12200"
     CDS             complement(<1..259)
                     /locus_tag="AC162G_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008338153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HIR5076627.1"
                     /translation="MAEVIRIENIARHVGQDVTVRGWLYDKTDKGKLSFLQVRDGTGI
                     VQAVVFAKDVSPELFDLSKRISQESSVEVTGTVRADARAPGI"
     gene            complement(341..1177)
                     /locus_tag="AC162G_12205"
     CDS             complement(341..1177)
                     /locus_tag="AC162G_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076628.1"
                     /translation="MRRKLTNTSNLYESQLLIWPTRPDVLVFYTTPGFPDGAGWIGHL
                     TVMRADGSNYEVVSREPVSSPAALSPDGYTIAYATYGEETSSVPWYYRLGSQPKRFPW
                     QDFGLAGFKNMGFSSPSWSNDGRRIAWWMRSYDDNYQRKFRGIGLFDLEAGEVVLLDN
                     FLQIVPDGWPQAVEWDADGKRIVFFGGRQQSELFGDWGEFGIWTADADGSHVRHLIGL
                     HSEWNPCDWAWQPDGEWLAFNCRDTDLAPGIWLVEIETGTLVKTNLPPGAEVRDWIRF
                     ER"
     gene            complement(2030..6322)
                     /locus_tag="AC162G_12210"
     CDS             complement(2030..6322)
                     /locus_tag="AC162G_12210"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HIR5076629.1"
                     /translation="MLRRRLPTLLILLGLFLLPLFLFWQVTLGGETLLPADIPFQFAP
                     WRSAAEQFDATIPDNPLLADLILENYAWKRFIVESLRAGEIPLWNPYLFAGAPFLANG
                     QASTLYPFSAIFYVLPLAQAYGWFTVSQLFLAGASMYVLGRMLGIGRFGSFFAAIVYQ
                     LSGFFVVSVVFTMIIAAAAWLPLLLAMIECVIQERSLLGRPATIPWVVIGAIALGCTI
                     LAGHSEIVYYTLLVMAFFAAWRLLSLRAEKPTGQQTRERPGRLVGWLAVVRRPIGWLL
                     LTVALGIGLGAVQLLPLGEVVTRNFREGSATLEQVRGWAWPPRHVLKFLMPDAYGNPA
                     HHSVFDVFTWQNVPLTSNLFCAQQPADPYCATWDRSTHFGIKNYVEGGTYLGILPLLL
                     AIFAVFRAQRTGQQTSRWAGRLVCWFKRPHVPFFTALALLSLAFAFGTALYAILYVLP
                     GINQLHSPFRWVWPYTLSVATLAGFGIEALIRDLDRDRAARQARGLLRWFTLLAPVSL
                     RTVLAGFAFWGGALLLAGLLVSRFVVPAQSLMAADRLLRALALADRAFPDAELFYSYE
                     WRNLLLLALFAMASGTLLRVSLCSIDIGKRNLAKVLDPSTALRAGPSQGVPIWKPLAV
                     GVLVLDLLIAGWGFNPSVDPALLDYEPPVVDFLRQDTSLWRFTTFDPHGRKTFNANLG
                     WPIAFQDVRGYDSVILKQYADYMRIIDQQDELQYNRIAPLTTYGGLDSPMLDLLGVKY
                     VLTDPDVPIESPKYRLVYDAEVKVYENLGAVPRAYAQTCAAGVEDQLDALRRIDPRTM
                     LILDAGVPGPNPARAACLLQPATITSYAPSEVFVEVTLDQPAWLVLNDTYFAGWQAWD
                     RLNGSAQETELTIQHANGNFRAVPLQAGQHTVRFKYSPWSFKLGAFISFMSAMVIVCL
                     VGVWLWRLAYREETVAGSTVRRVAKNSLAPMLLTLFNRAIDFAFAALMLRILRPENVG
                     NYALAVVIAGWVEILTNFGLNTLLTREVSKDLSQANRYLVNTSILRLMLGGVSIPGVL
                     LLLLIATGAPGLLGGNLTPETAITIVLLTVAQIPSSLSYGLTALFYANEKAEYPAAIS
                     VATVMLKVLLGVPALLVGWGIVGLALVSLAVNVVTLLILARQSFRLFFKPRVESDPAL
                     RRGMIRESFPLMLNHLLATLFFKIDIPLLYSLQGPAVVGLYSAAYKWVDALNIIPAYS
                     TIALFPVMSRQAGDDRAALLRSYRIGARLLVALALPLAVATTFVAPLLMLILGGHEYL
                     PHSATALQIMIWSIPFGWINSITNYVLIAAGQQSKLTRAFVVGLVFNVAANLILIPRY
                     SYVATAVVTIVAELVEGLAFQYYVEKSVGVVPWVRMLWKFFASAAAMFVVTWALWDVS
                     GVVALGAGIAVYGAGVWGLKAFGLEERAVFMELRRSRSESASQRVGESTASM"
     gene            complement(6339..>6523)
                     /locus_tag="AC162G_12215"
     CDS             complement(6339..>6523)
                     /locus_tag="AC162G_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HIR5076630.1"
                     /translation="VEMAYLCERLGLSVLEVPIYFEDRRIGQSKMSVPVKIEAAWRVW
                     QVWLRHRRVRRLSLDR"
BASE COUNT         1430 a         2058 c         1966 g         1069 t
ORIGIN      
        1 ggatgcccgg cgcgcgcgcg tcggcgcgaa ccgtgccggt cacttcgacg ctcgattcct
       61 gcgagatgcg cttcgacagg tcgaacagct ccggcgacac atccttggcg aagacgactg
      121 cctgcacgat gcccgtcccg tcgcgcacct gcaggaacga cagcttgccc ttgtcggtct
      181 tgtcgtacag ccaaccgcga accgtaacat cttgtccgac atgccgggca atattctcga
      241 tgcgaataac ctcagccacg atgtcctccg aagcgaagtg aggcgattct accatgaaac
      301 gtacagtgtt atgagtgaag cgtgatgaag aaatgaacca ctaccgctca aaccgaatcc
      361 aatcacgcac ttccgcgccg ggcggtagat tggtcttcac cagcgtgccg gtttcgattt
      421 ccaccagcca gatgccggga gcgaggtccg tatcgcggca gttgaaggca agccactcgc
      481 catcgggctg ccaagcccag tcacagggat tccattctga gtgaagcccg atcagatgac
      541 gtacgtggct gccgtcagcg tctgctgtcc agattccgaa ctccccccaa tctccaaata
      601 actcgctctg ctgacgaccg ccaaagaaca ctattcgctt cccatcggcg tcccactcaa
      661 ctgcctgcgg ccagccatca ggcacaatct ggaggaagtt atcaagcaga acaacttcac
      721 cggcctccaa gtcgaaaagg ccaatgcctc taaacttccg ctgataatta tcatcatagc
      781 tccgcatcca ccatgcgatc cttcggccat cgtttgacca ggacggagac gaaaaaccca
      841 tgttcttgaa acccgcaagc ccgaaatcct gccaggggaa acgcttcggt tgagagccaa
      901 gccggtagta ccagggcaca gaactcgttt cctcgccata agtggcgtag gcaatcgtgt
      961 aaccatccgg cgacagcgca gcgggacttg aaacgggctc tctcgaaacg acctcgtagt
     1021 ttgatccatc tgctcgcatg acagtcaaat ggcctatcca ccctgcgcca tcggggaaac
     1081 cgggtgtcgt gtagaatacc agcacatctg ggcgggtggg ccagatcaag agttgcgact
     1141 cgtagagatt ggacgtattg gtaagcttgc gcctcaagcc cgtcgcaacc tcctcgaccc
     1201 agatgtcggt aagacgattc tcgtcaggga gcgtgtaata tagagagcgg ctatggggca
     1261 accacactgc gtaccgcgag tcatatgata cagtccgtgt gtaacccgtg gtcaaatgca
     1321 tcaagattgc ctcatcgccg cacttgcagt ccctatatat gctccattgg cgatcggggg
     1381 agagcaaggc acgaggactg tcgatgattt tccgaggctg actggcggat tcgacaagcc
     1441 atattccgct ctcatccgaa tagacgatcc cggccggcgc aggtacgaca ctggctggag
     1501 tacttgtcag tgccagcgtc ggctttggcg cgggtggcga gagatgtgtg actgctggct
     1561 gcagtgtggg agcgtgaatg ggtgaagatg tgaccgtggg cgatgtctgc gctggggtcg
     1621 gctgggcagt cggagttgat acggcgactt caatcggggt cggcgtgacg acaacaatga
     1681 tcggttcggg tgaggattcg cacgccgcga agacagcgac taacagcaag aaccactgcc
     1741 aacgtcgggt gggcatgaca cacctccgca gcagacaatc gacaatctca ttctatgcct
     1801 gccggtgcgc gctgccggtc aactgcgcgg tgattatcct acgctaggcc aacgcatgcg
     1861 gggtctaagg tataatccgc ttcactcgcc gagcagcggc tgattgaggg gaagtagccc
     1921 ccccccgtcg aacaaaaccg gttcgacagg cgagggagcg gcccgtcccc tcccctgtgg
     1981 catcttttcg ccacggggga gggcctaggg agggggccaa cggttatctt cacattgacg
     2041 ccgtcgattc gccgactcgc tgactcgctg actcactcct cgatcgtctc aactccatga
     2101 acaccgcgcg ctcctccagg ccgaatgcct tcaaccccca cacgcctgca ccgtatactg
     2161 cgatgcccgc gcccaatgcc acgaccccac tgacgtccca cagcgcccac gtcacgacga
     2221 acatcgccgc cgccgaggca aagaacttcc acagcatccg aacccacggc acgacgccga
     2281 ctgacttctc gacgtagtac tggaacgcca gcccctcgac cagctcggcc acgatcgtga
     2341 ccaccgccgt ggcaacgtac gagtaacgcg ggatgaggat cagattcgcc gcgacgttga
     2401 ataccaaccc gacgacgaac gcccgcgtca gtttcgattg ctgcccggcg gcgatcagca
     2461 cgtagttcgt gatactgttg atccagccga agggaatcga ccagatcatg atctgcagcg
     2521 ccgtcgccga atgcggcagg tactcgtggc cgcctaagat gagcatcagc agcggcgcga
     2581 cgaaggtcgt ggcgacggcc agcggcaacg ccagcgccac gagcagccgg gcaccgatgc
     2641 ggtacgagcg cagcaaggcc gccctgtcat cgccggcctg acgcgacatc acggggaaca
     2701 gcgcaatcgt cgaataggcc gggataatgt tcagggcgtc gacccatttg taggcggcgc
     2761 tgtacaggcc gacgaccgcc gggccttgca gcgaatacag cagcggaatg tcgatcttga
     2821 agaacagcgt cgccagcagg tgattgagca tcagcgggaa cgactcgcgt atcatcccgc
     2881 gccgcagcgc cggatcgctc tcgacgcgcg gtttgaagaa caagcggaac gactgccgcg
     2941 ccaggatcag aagcgtgacg acgttgacgg cgaggctgac gagcgccagc ccgacgatgc
     3001 cccatcccac cagcagcgcc gggacgccca gcaacacttt gagcatcacg gtcgcgacgc
     3061 tgatcgccgc cgggtactcg gccttctcgt tggcgtagaa cagcgccgtc aacccgtacg
     3121 acaggctgct cggaatctgg gcgacggtga gcagcacgat ggtgatcgcc gtttccggcg
     3181 tgagattgcc gcccaacagc ccgggcgcgc cggttgcgat cagtaacaac agcagcacgc
     3241 ccggtatcga cacgccgccg agcatcagtc gcagtatcga cgtgttgacg aggtagcggt
     3301 tcgcctgcga aaggtctttg gagacctcgc gcgtcaacag cgtgttcagg ccgaagttgg
     3361 tcagaatctc tacccagccg gcgatgacca cggcgagcgc atagttgccg acgttctccg
     3421 gccgcaggat gcgcagcatc agcgcggcaa aggcgaagtc gatggcgcgg ttgaacagcg
     3481 tgagcagcat gggcgcaagc gaatttttcg ccacccggcg cacggtcgag ccggcgacgg
     3541 tctcttcgcg gtaggccagc cgccacagcc acacgccgac caggcacacg atcaccatcg
     3601 ccgacatgaa cgagatgaac gcgccaagct tgaacgacca cgggctgtat ttgaaccgaa
     3661 cggtgtgctg cccggcctgg agtggaacgg cgcggaagtt gccgttggcg tgctgaattg
     3721 tcagctccgt ctcctgggct gatccgttca gtcgatccca cgcctgccag cctgcgaaat
     3781 atgtgtcgtt aagcaccagc cacgccggtt gatcgagcgt cacttcgacg aagacttcac
     3841 ttggggcgta cgaggtgatg gtcgcaggct gcaagaggca cgctgcgcga gcaggattgg
     3901 ggccgggcac gccggcatcc agtatgagca tcgtgcgcgg atcgattctt ctcagcgcgt
     3961 cgagttgatc ttcgacgccg gcggcgcacg tctgggcgta ggcacgcggc accgcgccga
     4021 ggttctcgta caccttcacc tcggcatcgt acaccagcct gtactttggg ctttcgatcg
     4081 gcacgtcggg atcggtcaac acgtatttca cgccaagcag atcgagcatg ggcgagtcaa
     4141 gcccgccata cgtcgtgagc ggcgcgatgc ggttgtactg cagctcgtcc tgctgatcga
     4201 tgatacgcat gtagtcggca tactgcttga ggatgaccga gtcgtagccg cgaacgtcct
     4261 gaaaggcaat cggccagccc aggttggcgt tgaacgtctt gcgcccgtgc ggatcgaacg
     4321 tcgtgaagcg ccacagggac gtgtcctgcc tgagaaagtc gacaaccggc ggctcgtaat
     4381 cgagcagcgc tggatcgacg gaggggttga atccccaacc ggcgatcagg agatcgagca
     4441 cgagcacccc gacggccagc ggcttccaga tgggcacacc ttgcgaaggg cctgccctga
     4501 gcgcagtcga agggtctaga accttcgcaa ggttgcgttt gcctatgtcg atcgaacaca
     4561 gcgacacgcg caggagtgtc ccgctcgcca tcgcgaacag cgccagcagc agcaagttgc
     4621 gccactcgta cgaatagaac aattccgcat cagggaatgc gcgatcggcc agcgccagag
     4681 cacgcagcaa ccggtcggca gccatcagcg attgcgccgg cacaacaaac cggctcacca
     4741 acaggccggc cagcagcagc gccccacccc agaacgcgaa accggccagc accgtccgca
     4801 gcgacaccgg cgcgagcagt gtgaaccatc gcagcagccc acgcgcctgg cgggctgccc
     4861 gatcccgatc gagatcgcgg atcaatgcct cgatgccgaa ccccgccaat gtcgcgacac
     4921 tcagcgtgta cggccacacc catcgaaatg gcgagtgcag ttgattgatg ccgggcagca
     4981 cgtagaggat ggcgtagagc gccgtcccaa acgcgaaagc caatgacaag agtgcaagcg
     5041 ccgtaaagaa ggggacatgc gggcgcttga accaacacac caaccggcca gcccacctgg
     5101 atgtttgttg acccgttcgc tgcgcacgga acacggcgaa gatcgctagc aacagcggca
     5161 agatgccgag ataggtcccg ccctcgacgt agttcttgat gccgaagtgc gtgctgcgat
     5221 cccacgtcgc gcagtacgga tcggctggct gctgcgcgca gaacaggttg ctcgtcaggg
     5281 ggacgttctg ccaagtgaag acgtcgaaga ccgagtgatg cgccgggttg ccataggcgt
     5341 cgggcatcaa gaacttcagc acgtggcgcg gcggccacgc ccaaccccgc acctgctcga
     5401 gcgtcgccga gccttcgcgg aagttccggg tgacgacctc acccaacggc agcagttgaa
     5461 cagcgcccag cccgatgccc agagccaccg tgagcaacag ccagccgatc gggcgacgca
     5521 ccacagccaa ccatccaact aaccggccag gacgttcgcg agtttgctgg ccagttggct
     5581 tctcggctcg cagactcagc aagcgccatg ctgcaaagaa cgccatcacc agcagcgtgt
     5641 agtacacaat ctctgaatgc ccggcgagga tcgtgcagcc cagcgcgatc gcaccgatca
     5701 ctacccatgg gatcgtcgcc ggccggccga gcagtgatcg ctcctgaatc acgcactcga
     5761 tcatcgccag tagcagcggc agccacgccg ctgctgcgat gatcatcgtg aacacgacac
     5821 tgacgacgaa gaagccgctg agctgataaa cgatcgccgc gaagaaactg ccgaagcgcc
     5881 cgatgcccag catgcgcccc agcacataca tggacgcgcc ggcgaggaat aactggctca
     5941 ccgtgaacca gccatacgcc tgcgccagcg gcagcacgta gaagatcgcg ctaaatgggt
     6001 acagcgtcga cgcctggccg ttcgccagaa acggcgcgcc cgcaaagagg tacggattcc
     6061 atagaggaat ctcgcctgcg cgcagcgact ccacgatgaa gcgcttccag gcgtagtttt
     6121 cgagaatgag gtcggccagg agcgggttgt cagggatggt cgcgtcgaac tgctctgccg
     6181 ccgagcgcca cggagcgaac tgaaacggga tgtcggccgg caacagcgtc tcgccgccga
     6241 gcgtgacctg ccagaacagg aacagaggca ggagaaacag gccgagcaag atgagcagag
     6301 tgggcaggcg gcgacgcagc atgaaaggag ggtgtggatc agcgatcgag agacagccgg
     6361 cgcacgcggc gatggcgcag ccacacctgc cacacgcgcc aggcggcctc gatcttgacg
     6421 gggacgctca tcttcgactg gccgatccgg cgatcttcaa aatagatcgg tacctccagg
     6481 acgctgaggc caagccgctc gcacaggtaa gccatctcga cct
//
LOCUS       DBNHXB010000304         3729 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_750687, whole genome shotgun sequence.
ACCESSION   DBNHXB010000304 DBNHXB010000000
VERSION     DBNHXB010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_750687"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            539..2542
                     /locus_tag="AC162G_12220"
     CDS             539..2542
                     /locus_tag="AC162G_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5076631.1"
                     /translation="MPARTKRLSTAEDLYRLEQITDCRIAPDGRHVVYAVQRVDKKTE
                     KKYANLWIVPMRGGAPRSFTFGDHVDAQPRWSPDGEHIAFLSNRGDEKQMQIYVIPFG
                     GGEARKLTDLKGSFGSFAWSPDGRQFVVQFRKKDREALEREQDEEKKKLGVVSRRITR
                     VWHKLDGEGFLPDERWHIWTVNARSGRAKQLTDGEVHDEVDPCWSPGGRSIGFVSNQH
                     PDPDFNPDEVDLFVMPATGGRPRKIGTPIGPKRMPVYSPDGKWIAYIGREGRGEWWKN
                     DRVYVAAADGKRKPVDLTGQYDIHLESGTLTDTGAPPTIPPVWSSDSRTIYFQVTRHG
                     NTELRSVSIDGGDLQTVIGDKGVVGAFSFSRDQATLAYFYAEMSDPGQVWARDMGSGS
                     SRRLTAVNAWLRGIDLGEMEEVWFEGAAGNRLQGWILKPPRFSARKRYPAIVEIHGGP
                     LLQYGHHFMHEFHFLAANGYVVAFSNPRGGQGYGEAHAKAPWQAWGTSDYEDIMAWAD
                     TVEKRPYVDKKRMGVTGGSYGGFMTNWIIGHTDRFKAAVTQRSISNQISMWGTSDFNW
                     FFQQEIGDKPPWENLEGYWEQSPLKYIGNAKTPTLISHSEQDLRCSIEQAEQMFVALK
                     KLGVDTEMVRFPDEPHGLSRTGRTDRRIVRLNHFLRWFDKYLK"
     gene            complement(2608..2835)
                     /locus_tag="AC162G_12225"
     CDS             complement(2608..2835)
                     /locus_tag="AC162G_12225"
                     /inference="COORDINATES: protein motif:HMM:NF019540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HIR5076632.1"
                     /translation="MTRLPSVTSRQMIQALQRAGFEVDHQTGSHVVLRRAPDGRRVVV
                     PMHNRDLGRGLTLQIVKSAGLSRDEFVELLK"
     gene            complement(2835..3077)
                     /locus_tag="AC162G_12230"
     CDS             complement(2835..3077)
                     /locus_tag="AC162G_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010901181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR5076633.1"
                     /translation="METYHFTVIIERQPEGEYLVTVPALPGCFTEGRTLEEARAMAVD
                     AISAYCVSLRKHAEPIPVEAFDEQFIGRVSVELEAA"
     gene            complement(3103..>3729)
                     /locus_tag="AC162G_12235"
     CDS             complement(3103..>3729)
                     /locus_tag="AC162G_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5076634.1"
                     /translation="GQGRTVDFRNTVIIMTSNLGTEFVRKSGALGFVRPGDSSGPAQV
                     ADHKKIEDELKRAFRPEFLNRVDEIIIFKTLTLEDVEKIVDLQMNEIRGRLRDQGLFI
                     ELTDPARKWLAKEGYDPQFGARPLRRALQRFVESPLSVAMLKGEFKIGDTVVVDTNEQ
                     GITFGHAVKVPEAEPELAGAESRPRSKSRATRGAPPRDTVPTDTDKVE"
BASE COUNT          719 a         1190 c         1171 g          649 t
ORIGIN      
        1 gtcagggctg ggggccggac ggcacaggtg aggcgatcct ggccgtggcg gcgcagcccg
       61 acggatcgac gtattggcat ggcatgattt acgcgctcgc agggttcgat tagacggcca
      121 gccggcacat ggcactctac actggctgga gtttcggcct ggcatggctc agccggtatg
      181 tgctgacgcg gtgagacgca gaaccccggt ttctcggaga aaccggggtt ctcagttgta
      241 tctccattgc atcgacattg ctcaaggaga taagaggcgc gtcgtcgcat ccaccagccc
      301 gcgaaggcgg gcttcgcgca gttatcggcg cagtttcaac cgccgccggc cagcgcgtgg
      361 cgctctaccg ccacaccaga ggataccgtt cgggatgctc cagtgcgtcg agttcaagat
      421 cggcatccag agctggccag tgaaactgcc ggggcgacaa tcgctgcacg ttgaatatgt
      481 gttaaacttg ggcgctggcg cgaagcccag cgatgacttc aatacccaag gagcctccat
      541 gcccgcacgc accaagcgtc tcagcactgc cgaagacctg taccgcctgg aacagatcac
      601 ggactgtcgg atcgcgcccg acggcaggca cgtagtgtac gccgtgcagc gcgtcgacaa
      661 aaagaccgag aagaagtacg ccaacctgtg gatcgtgccc atgcgcggcg gcgcgccacg
      721 gtcgttcacg ttcggcgacc acgtggacgc gcagccgcgc tggtcgccag acggcgagca
      781 catcgccttc ctgtcgaatc gcggcgacga gaagcagatg cagatttacg tcatcccgtt
      841 cggcggcggc gaggcgcgta agctcaccga cttgaaaggc agcttcggct ccttcgcgtg
      901 gtcgcccgac gggcggcagt tcgtcgtcca gtttcgcaag aaggatcggg aagctctgga
      961 acgcgagcag gacgaggaga agaagaaact gggcgtcgtc tcgcgccgca tcacgcgcgt
     1021 atggcataag ctcgacggcg aggggtttct gccggacgag cgctggcaca tttggacggt
     1081 caacgcgcgc agcggcaggg cgaagcagtt gaccgatggc gaggtgcacg acgaggtgga
     1141 tccgtgctgg tcgcccggcg gcaggtcgat cggcttcgtg tccaaccagc atcccgatcc
     1201 ggacttcaac ccggacgagg tcgacctgtt cgtcatgccc gcgaccggcg gccggccgcg
     1261 caagatcggc acgccaatcg gccccaagcg catgccggtc tattctcccg acggaaagtg
     1321 gatcgcctac atcgggcgcg aagggcgagg cgaatggtgg aagaacgatc gcgtgtacgt
     1381 cgcggcagcg gatggcaagc gcaagccggt cgatctcaca ggccaatacg atattcacct
     1441 cgaaagcggg acgctcaccg ataccggcgc gccgccgacg ataccgcccg tatggtcgag
     1501 cgacagccgg acgatctatt tccaggtcac gcggcacggc aacaccgagc tgcgctccgt
     1561 gtcgatcgac ggcggcgacc tgcaaacggt gatcggcgac aagggcgtcg tcggcgcgtt
     1621 ctccttcagt cgcgaccagg ccacgctcgc ctacttctac gccgagatga gcgaccccgg
     1681 ccaggtgtgg gcgcgcgata tggggagtgg cagctcgcgg cgtctgacgg ccgtcaacgc
     1741 ctggctgcgc ggtatcgatc tgggcgagat ggaagaggtc tggttcgagg gcgcggccgg
     1801 caaccggctg cagggctgga tcctgaagcc gccgcgcttc agcgcccgca agagataccc
     1861 ggcgatcgtc gagattcacg gcggcccctt gctccagtac ggccaccact tcatgcacga
     1921 gttccacttt ctcgccgcga acggttacgt cgtggccttc tccaatccgc gcggcgggca
     1981 gggctacggc gaagcgcacg ccaaagcgcc gtggcaggcg tggggcacct ccgactacga
     2041 agacatcatg gcctgggccg acaccgtcga aaagcggccg tacgtcgaca aaaagcgcat
     2101 gggcgtcacc ggcggcagct acggcgggtt catgaccaac tggatcatcg gccacaccga
     2161 tcgcttcaag gcggccgtga cccagcgcag catcagcaac cagatcagca tgtggggaac
     2221 gagcgatttc aactggttct ttcagcaaga gatcggggac aagccgccgt gggagaatct
     2281 ggagggctac tgggagcagt cgccgttgaa atacatcggc aacgccaaaa cgccgacact
     2341 catctcacac agcgagcaag acctgcgctg ctcgatcgag caggccgagc agatgttcgt
     2401 cgcgctcaag aagctcggcg tcgacacgga gatggtgcgg ttccccgacg agccgcatgg
     2461 cctgtcacgc accggccgca ccgaccggcg catcgtgcgg ctcaaccact tcctgcgctg
     2521 gtttgataaa tatttgaagt agacaggtag acaggtggca agtaaaaagg cacggagggg
     2581 ttacgcctct ccgtgcctgt gtgtttgtca tttgagcagt tcgacaaact cgtcgcgact
     2641 cagccctgcg ctcttcacga tctgcagcgt cagcccacgt cccaggtcac gattatgcat
     2701 tggcaccacg acacgtcgtc cgtctggcgc gcgcctaagc acaacatgac tgcctgtctg
     2761 gtggtcgact tcgaagccag cgcgttgcaa ggcctggatc atctgccgag acgtgacgct
     2821 cgggaggcga gtcattaggc tgcctcaagc tcaacgctca cacgtccgat aaactgctca
     2881 tcgaaagcct cgaccggtat gggctcagcg tgcttgcgga ggctcacaca gtacgcgctg
     2941 attgcgtcaa cagccatcgc acgcgcttct tccagcgtcc ggccttccgt gaaacatccc
     3001 ggcagcgccg gcacggtcac cagatactcg ccctcgggct gacgttcaat gatcacggta
     3061 aaatgatatg tttccatcgt ccatccccga tagcgcgatc tctcattcca ccttatccgt
     3121 atccgtcggc actgtgtcgc gcggcggcgc acctctcgta gcgcgcgact tcgaacgcgg
     3181 tcggctttcc gctcccgcca actccggctc ggcctccggc actttgaccg catggccgaa
     3241 cgtgatgccc tgctcgttcg tatccacgac aaccgtatcg ccgatcttga actcgccctt
     3301 cagcatcgcc accgagagcg gtgattcgac gaatcgctgc agcgcgcggc gcaacgggcg
     3361 cgcgccgaac tgcgggtcgt agccttcctt ggccagccac ttgcgcgccg ggtcggttag
     3421 ctcgatgaac aggccctggt cgcgcaggcg gccgcgaatc tcgttcatct gcagatcgac
     3481 gatcttctcg acgtcttcga gcgtgagcgt cttgaagatg ataatctcgt cgacgcgatt
     3541 gaggaactcg ggccggaacg cgcgcttcag ctcgtcctcg atcttcttat gatcggcaac
     3601 ctgcgccggg ccgctcgaat cgccgggccg cacgaagccg agcgcgccgc tcttgcgcac
     3661 gaactcggtg cccaggttgc tggtcatgat gatcaccgta ttgcggaaat cgactgtgcg
     3721 cccctgccc
//
LOCUS       DBNHXB010000305        32310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1589796, whole genome shotgun sequence.
ACCESSION   DBNHXB010000305 DBNHXB010000000
VERSION     DBNHXB010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 32310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 32310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32310
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1589796"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="AC162G_12240"
     CDS             <1..486
                     /locus_tag="AC162G_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HIR5076635.1"
                     /translation="ARGGDVLAPGRPDRQIQFIDVRDLAEWMIRAVEQNLTGVYNANG
                     PDDHLSMGTLLETSKVVSGSNAHFVWVEDQFLIDSGVQPWSEVPLWIPESDPDAAGFF
                     AFDCRKAIAAGLTFRPLEETIRATLEWETTRSTEYEWRAGLKPEREAELIGIWSDHVI
                     T"
     gene            712..2424
                     /locus_tag="AC162G_12245"
     CDS             712..2424
                     /locus_tag="AC162G_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5076636.1"
                     /translation="MSARKWCLTLVLALALIASACGPAATPTPTEPPAAPATQPPAPP
                     TEPPPTPTEPPARKVATFIWTQEFDTLNPYYTNMWFSTITHQFWNCWPWIFDDQNNLV
                     PVLVEELPSTENGGISADGKTLTMKLRDDITWSDGTPITADDFVFTYEMVINPKNIVA
                     STDPYDKMESVTAPDPQTVVVTFKDAYAPWAGSLWHGILPKHVLEPVLEKDGTLDNAE
                     WNRNPTVGCGPYNFAEWESGSFARFVARDAYWAGRPKIDEIFIRFVPDDASQVAALKA
                     GDGDLGTFIAYSDVPDLEAAGVAMYKAFSGYNEGIYFNLSSEKGHPALQDQRVRQAIA
                     LATDRESLNKDLLLGLTQPAATDWDNTPYIDPSIDPWPYDPEQAKQLLDEAGWVDSNG
                     DGVRDKDGVELVLKYGTTTREIRMDTQAVLQQQLAEVGIKVELLNFESDIYFGGYADG
                     GPAATGQLDIFEYSNSPQFPDPDTAEWLCSEIPTDEYPDGTNWSLYCDEELDALFQKQ
                     ATQVDFAERQATFYQITKMIFDQVYWLGLWQDPDIWAVNSRLKNVKFSGTTPFFNIIE
                     WDIE"
     gene            2536..3567
                     /locus_tag="AC162G_12250"
     CDS             2536..3567
                     /locus_tag="AC162G_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076637.1"
                     /translation="MTRYIIRRLLQAIPLLFIVSVVIFVLMDSAADPLATMGGRKVTR
                     AEDRARLTRQLGLDQPLHMQYLFWLIGNDWTKTDLDGDGVAETPGTRKGMLRGDFGTS
                     LVERRPATAVILDRLPNTLLLMLAAEVVIICFALIIGTYSAMHQYSLTDNTITALSFI
                     FYSMPIFFIALGSIYVFAVNFEKWGLPYLPTNGMFDPQVGETPQQVAWHMVLPVLSIA
                     LISMAAYSRYIRSAMLEVMNQDYIRTAKSKGLARRYIIWVHALKNASLPIVTLVGLDL
                     PLLLAGALVTERIFAWPGMGRLFLDHVERGDVSVVMGILMLITLAVVVFQIITDIVYA
                     WLDPRIRYA"
     gene            3590..4543
                     /locus_tag="AC162G_12255"
     CDS             3590..4543
                     /locus_tag="AC162G_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076638.1"
                     /translation="MSTAIEAGRIPRRSADEIESPPLTLRQLAGRRFRRHKMAMFGSV
                     MLIVLILWSLGGALFYTEAYANYNDTSLRLQDPSAEHPFGTDTIGRDILARTIYGGQI
                     SLLIGITAVIIEVIVGVLIGAIAGYYGGRADAVLMRLTEAMLCIPQIFLLIVMAKFFG
                     GKIPNVTILGREFSGSVVVIVLIIGLTSWPYLARIVRAEFLALKEREFTLAARATGTQ
                     PRSIIFRHILPNCAAPIIVSATLGVANAITTEAYISFLGLGVRPPTATWGNMLESSYN
                     YIESAPWLWFFPGMLILLTVLSINFVGDGLRDALDPRSRVV"
     gene            4691..5677
                     /locus_tag="AC162G_12260"
     CDS             4691..5677
                     /locus_tag="AC162G_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076639.1"
                     /translation="MTTLLEVSDLRTRFHTPEGTVYAVNGVSFDLSEGETLAVVGESG
                     CGKSVTMLSILGLIPKPPGEIVTGSALYRGVDLFKVTEQQLEHIRGKDIAMIFQDPMT
                     SLNPVLTIGRQITESLRMHMGMSADQARERTIQLLGMVGLSDPVRRLGDYPHQFSGGM
                     RQRVMIAMALACAPSILIADEPTTALDVTIQAQIVELAIRMREQMGMALIWITHDLGV
                     VASIADRVIVMYAGYIVEEAGIDAMYDDPRHPYTLALRAALPRHDRRRDHRLKSIPGA
                     PPSLQVKPHGCPFAPRCEFALERCWEENPTLTPVDQNHRIACWVDVTTGAPR"
     gene            5674..6669
                     /locus_tag="AC162G_12265"
     CDS             5674..6669
                     /locus_tag="AC162G_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076640.1"
                     /translation="MSTAEPLVVVENLKKHFPVMRGIVFQRQAGAVRAVDDISFTIAR
                     GETLGLVGESGCGKTTAGRTLLNLYPPTSGKVTIDGMDVYAAKGRDQMIIRRKSQIIF
                     QDPFASLNPRWTVNAIVGEPLRVHKLRRTGTERTERVKELLELVGLSARHLNRFPHEF
                     SGGQRQRIGVARALASEPLFIVCDEPISALDVSIQAQVVNLLEDLQDRLGLTYLFIAH
                     DLSMVRHICDRVAVMYLGMIVELAPRDDLYENPLHPYTQSLLSAVPIPDPKKERARKR
                     IILTGDVPSPIDPPSGCRFHPRCPIAKDHCSKIVPEWREIKSGHWVACHEARLTE"
     gene            6858..8579
                     /locus_tag="AC162G_12270"
     CDS             6858..8579
                     /locus_tag="AC162G_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5076641.1"
                     /translation="MWKRAWYLTSLLLVAALVGSACGGAATTPPAPTTAPPAETQPPP
                     PTEPPPATQEPAILRIGWSGAPDSLNPAAGLLTESYTLYELTYDTLIGLDLSGTYYPE
                     LAESWEVSDDGTVWTFHLREGVKFHDGTDFTAEDMAFSIQFYKDHEEFPYLNSYTLNF
                     ETVEATDDATLVITLGEPIGNMESQVLFLYALPKHIWEGIGFDEALTFENLEMIGTGP
                     FKVVKYVRDQSIELAANKDHYRNPPKIDGVIFQTYESSDALVQALREGQADLITDVPP
                     TTIASLKQEANITAQVGGSRSLRDIILNVVPDDKCPAEGGVCSGHPALKDRVVRQALA
                     HATDKQQIIDVALLGLGTPGLSMVPRSLGDWFNSELEDYAYDVDLANKMLDDAGYKDT
                     DGDGVREMPDGTNPLKFRFLIPSDILTGAREGDLIKAMWNEAGVDIDIQVLEPDTVTA
                     ACCPAFDYDVLLWGWSADPDPAYLLGVLTTDEIDTGFSETGYSNPVYDQLYLDQSTAV
                     DHEARKKIIWEMQKIALEDVPYIIPYYQNNVMAYRNDKFTGYVYVEGGLLSLDDPLSL
                     SNVEPVR"
     gene            8636..9667
                     /locus_tag="AC162G_12275"
     CDS             8636..9667
                     /locus_tag="AC162G_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076642.1"
                     /translation="MGRRNYLLRKAGASLLTIFIVLVINFFLFRVIPGDPAKAGLKDP
                     RVRPEVIEAQRVRFGLDKPVINCFKGQMVFDASRCAVNPLDTQLFIYLGNLFKGDLGI
                     SYDQKRPVADILRERIGNTVILLLGAELFAIILGSLLGLVAGWKRKTRLDTSALIFSL
                     TAWSLPTFWLGILMFMIGSSAFGLPVNGMRTTGLTYANFGEELADVARHWLLPSLTYA
                     IVLLGQYMIVMRASVLEVLSEDYILTAKAKGLTNFQILKDHALKNAMLPMVTLVLLNL
                     GFTVSGAVELERVFSWPGVGLAIVEAVARKDYPVLQGAFLIVTISVIFANLLMDLLYT
                     YLDPRVKAE"
     gene            9672..10616
                     /locus_tag="AC162G_12280"
     CDS             9672..10616
                     /locus_tag="AC162G_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076643.1"
                     /translation="MNRFSRWRDGFKNFWRVFRHNRLGLLGLIMLAAAIVIAVFAPLI
                     APADPYARVRVKVEDIYAPPSAAHPFGTDDAGKDVLSAFIYGSRVSLIVGFAASLISL
                     FVGGAVGLIAGFYGGRLGNFLMRINDIFLVIPFLPLAIVLVALLGQSLLNIILVIGLL
                     GWSSTARLVRSQVLSVKERQFVTRARAIGAGNLHIIWHHILPLVLPLLLANTVLIISL
                     SILAESSLAFLGLGDPTVISWGSMLNFAFTRSAMSAGAWWALVLPGLGIVWVVLACTL
                     LGNVLEEIFNPHLKAHHLSSTDKMVAIPNEIQPAPSGK"
     gene            10585..11667
                     /locus_tag="AC162G_12285"
     CDS             10585..11667
                     /locus_tag="AC162G_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076644.1"
                     /translation="MKFNPRRLGNDPASIALDWQIDNMSSPAPLLEVKNLSTHFLTDD
                     GVMHAVEDVSFTLDRGQSLGLVGESGCGKTTTMLALMRLLPPNGRIVKGQVLLEGRDL
                     LRLPDSEMQRVRWSKISLIFQGAMNALNPVRTVGDQIAESIRRHGLMGGRLDNHALDQ
                     RVRQLLDMVGIASSRQDQYPHQYSGGMRQRAMIAMALACTPDVVIADEPTTALDVMIQ
                     AQILDLLQKLQRELGLAVILITHDLAVVAEMCDRVLVMYGGKVAEYADVDAIYNEPLH
                     PYTRLLLAAFPDLTQPEGKLVAIPGYPPRLNALPAGCRFEPRCPVRIGKCVTADPPLR
                     EVKPGHWVACHLVTEQLPIHPTTSLP"
     gene            11679..12656
                     /locus_tag="AC162G_12290"
     CDS             11679..12656
                     /locus_tag="AC162G_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076645.1"
                     /translation="MLEITRLRKHFPISRGLLASFRRRSAHSVHAVDDISFQVQPGEI
                     LALVGESGCGKTTTAKLLMGLEPPSGGTIHFCGRDVATFDLKQMRRQIQMVFQDPYES
                     LNPRMTVFEIVAEPLNVHHLARSREDRIDRVKRALKDAGLKPAESYFNRLPHELSGGQ
                     RQRVVIAAALVLEPTVLLADEPVSMLDVSIRADILNLLAELRQTRGISVLFITHDLGT
                     IAYFADRIAVMYLGRIVEIGPARDVLSNAQHPYTKALLSVVPVPNPRLRRERIILQGE
                     TPNPIDLPGGCRFHPRCPVAIEQCRQIDPPYVELPNSHRAACLLIESTQ"
     gene            12890..13099
                     /locus_tag="AC162G_12295"
     CDS             12890..13099
                     /locus_tag="AC162G_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR5076646.1"
                     /translation="MSERITGTVKWFSRTKGYGFITQPNGPDVFVHYSAIQGEGFRNL
                     NEGQQVEFTVLEGPKGLHAADVRAV"
     gene            complement(13483..14715)
                     /gene="metK"
                     /locus_tag="AC162G_12300"
     CDS             complement(13483..14715)
                     /gene="metK"
                     /locus_tag="AC162G_12300"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR5076647.1"
                     /translation="MTNSFMRSPQLYFTSESVSEGHPDKMCDQISDAVLDAMLSQDPR
                     SRVACEVSATTGLIVVMGEVTTQAHLEVQDVVRQTVADIGYTDAAYGFDGRTCGVVVS
                     LKGQSPDIAIGVDRAAEAKSGEMTGSEIEAVGAGDQGMMIGFACDETPELMPLPIALA
                     HRLVKRLSHARRNGEIEWLRPDAKSQVTVEYAYGKPKRVDTVLVSTQHDERVTHKQIR
                     DDVIERVIREVIPADLLDARTKYYVNPTGRFVIGGPMGDAGLTGRKIIVDTYGGMARH
                     GGGAFSGKDPTKVDRSAAYYARYAAKNLVAAGVADRLEIQVSYAIGMARPLSIMVETF
                     GTGKVPEEKILGILRAHFDFRPGAIIQNLNLRRPIYRPTAAYGHFGRHDIEAPWEVTD
                     KADAIRAEAGLEPAFVAA"
     regulatory      complement(14815..14978)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            15102..15175
                     /locus_tag="AC162G_12305"
     tRNA            15102..15175
                     /locus_tag="AC162G_12305"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15136..15138,aa:Val,seq:cac)
     gene            15305..17026
                     /locus_tag="AC162G_12310"
     CDS             15305..17026
                     /locus_tag="AC162G_12310"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR5076648.1"
                     /translation="MRTIKPLYQIDGRLANLRWFLLITAGIVIALQAQVTGSDLPALQ
                     IAAVIVACIFNLLIVLSLAVDMSPRPVPLIGLAVDTMLAIILISSDPRMTSPLIFYAI
                     FPIITSALRFGWLFNVVTAGAIMGAYVAFKMIGRGLAGEQVLQTLGTLLILGVAATVS
                     GTIGGRVKDLVLQAMRTESEGELRQLRTRREQARAIFEMASTLSATLSYERILEAVLD
                     VGAMGLREFGSVAQRLVSIIMLFTEDGLVVSASRRLTARDEAARLTAPSGIIAQALQS
                     AEPIICQDPKGDPDLATFVALHRCVTVVVVPLRAGFESYGVAVFGSPEPDVFTPDHLE
                     LLTVVASQAIIALQNAQLYQKLQEEKNRIVDIEEDARKKLARDLHDGPTQNVAAIAMR
                     LNYTRTLLKRSPARAEEEIEKIEDLARRTTKEIRYMLFTLRPLVLETQGLVPALEQYV
                     QKFKETDDSVEIILEAGRITDRVSRDAQGAIFYIVEEAVGNARKHAQAQHIWINLHED
                     SSQLTAEVRDDGIGFEVQAVQKSYDQRGSLGMVNMTERAALVKGTLSVASAPGEGTHV
                     TLVMPLK"
     gene            17075..17755
                     /locus_tag="AC162G_12315"
     CDS             17075..17755
                     /locus_tag="AC162G_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076649.1"
                     /translation="MYVVAARLVAPRIGAALRVSPPGQWLLEGAQFAYFLGVPYLALL
                     TGAFAPSDVGLQGSPALDLLLGWAAEEWMRALGQAIALGLLTLLVVRLLTWQIRRAGG
                     CGAAAMEIVDASAAHSIRDGIYAEVHWSFYRALPMVLWGDAGRAALLGIALAAAEALL
                     AGRRTTSSVSRISASSALLGMGSATFFALTGGNFWIASVLQVTMRVSLARIAPAPERA
                     EPLEDVIV"
     gene            complement(17887..18189)
                     /locus_tag="AC162G_12320"
     CDS             complement(17887..18189)
                     /locus_tag="AC162G_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076650.1"
                     /translation="MAKKTTRQRKELSRQRAVQQAAANVTAGIAQPASPASTPTPIPA
                     VRTGVDPSAALRARPERSEGAGVGEEYAYVRSDLRRIAILAAAIVALLVVLSLVMA"
     gene            complement(18212..19162)
                     /locus_tag="AC162G_12325"
     CDS             complement(18212..19162)
                     /locus_tag="AC162G_12325"
                     /inference="COORDINATES: protein motif:HMM:NF016119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HIR5076651.1"
                     /translation="MPDRRSVDDLSIEELEQILAIRKRQAREATLRRLQGEGRIVDGE
                     FANEVLSRPATSAGEAPADPIGSRYRTAEIASPSETRRSRVFPWRAVRDRFLLLIEIG
                     ALIGLVLVVVGIEQTRQELNRDVAVARNVGQSSAEPTPVIRAVVLPSGHKPPTSPGGA
                     APNFDEIPAHLRAYVQSVTPLPIPTRGPGQPTRIVIPALGVDGNVVQGTDWEQLKKGV
                     GHYLNTANPGERGNLVLAGHNDVFGEIFRDLDKLKVGDEIMIYSVDRAFRYVVTETRI
                     VEPTQVEVMDPTSEPTATLISCYPYLVDTQRIVVFAALQS"
     gene            complement(19200..20012)
                     /locus_tag="AC162G_12330"
     CDS             complement(19200..20012)
                     /locus_tag="AC162G_12330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5076652.1"
                     /translation="MSSEQTQASLPSTLYTEDYFLTACEGYEEFVESHGEHLSRRLNA
                     ALAVASVEPGMRVLDVGCGRGEILRHCARLGARAYGIDYADVAARLSHDVASTEVEST
                     GRPAIGVYRADAKFLPYPDAAFDRVLIFDVVEHLYPWELDAALREARRVLKPGGKIAI
                     HTAPNRWYDRYAYPVVRLVRTMQGRSTQYPANPRALNVAANVDVHVNEQDMRSLHRNL
                     KQAGFAHVRVWLDSPPQNRQESRIFRLLRRVAFGLPPFRWFFEREVFAVGTK"
     gene            complement(20113..21738)
                     /gene="murJ"
                     /locus_tag="AC162G_12335"
     CDS             complement(20113..21738)
                     /gene="murJ"
                     /locus_tag="AC162G_12335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR5076653.1"
                     /translation="MSELLETSEIPITPEAASETLVIARAASIIALGNVASRVLGLAR
                     EAVKANLFGSTGLVSAYQAAALVPTLLFDLLIGGMVSSALVPAFSEVAQRDRNALWRL
                     ASLVMTLAFVVLTALCLVVIVAAPFVARLISGGLDARLLAQTSSLLNVTAVATIFISL
                     SAILSGLLQALKRFTLPAFTGAVFNAAIVVCALVLGSRFGIASMAIGLLAGAILQVVF
                     QLPSLRDARLRPAWDLRHPGLRQIGRLYLPVVLGLAINQLAIVLSYNLASRASEEGIA
                     WMQYATTLIQFPLGLVATAVSIAILPTLSRQASLDNTGGEASFKATLARGLRLVMVLI
                     VPAAVGLLVLARPIVALLFEHGQFATSDTDAVTRVLQFYVPGLAFAAVDLLLVFAFYA
                     RKDTLTPALVGLVCNVVIYLAAALAPGVILGRPLEVVDLAFANSVQWICHALIMLWLL
                     ERRIGGLRGFGLRALTLKALVGSAAMGIATWIVVALLGSSQYLEAYPGFVAEVITVAG
                     AALAGAAVYLGVMAVLHAGEMTHWGGLIRRRVA"
     gene            complement(21912..22145)
                     /locus_tag="AC162G_12340"
     CDS             complement(21912..22145)
                     /locus_tag="AC162G_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076654.1"
                     /translation="MKQTLVRHMILVLAITAPTWFGVASGIALDTVIPLQSVVVLIVA
                     AGDLIGPDVQVAEEVPASTQEDAPRRRSTRRDK"
     gene            complement(22489..24354)
                     /gene="uvrC"
                     /locus_tag="AC162G_12345"
     CDS             complement(22489..24354)
                     /gene="uvrC"
                     /locus_tag="AC162G_12345"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR5076655.1"
                     /translation="MPSDQVAEKLKTVPSRSGVYLYRDARGAILYVGKAINLRNRVRS
                     YFHNSIDPFSKTGRLVMEIADLEWIITETELEALLLEANLIKKHRPRFNILLKDDKRY
                     PYVKVEWQNDFPKVLTVRKMERDGARYFGPYTNAGAVYATLETLRRIFPYLTCDRIIT
                     GKDPRACLYYDIKLCGGPCIGAVSKEEYRATIDRLCAFLEGKTDQVVAEIEKKMQAAS
                     EGMQFERAAEYRDQLRAIRHIAERQKVVSTMGKDQDVVAFARDEKRGDACVQVFFIRQ
                     GKLIGREYFILEGAEGEDTREIVSSFLKQFYEEAATVPPEILLQVEVEEAQIIQQWLR
                     QKRGAQVLLDVPREGQPADLVAMAAENAAETLSALQAQWMADEHKNEQALQELADGIG
                     LEALPARIECFDVSNTQGTNTVASMVVFVKGVPRKSHYRKFNIKSVDGPDDFASMREV
                     LTRRLRRYFDAKAQESQPAPGKKKDESFSVLPDLLIVDGGKGQLGVAVEVLKEFDLFG
                     QIPVCGLAKQQEEIFLPGHPDSILLPRRSQGLFLMQRIRDEAHRFAITAHRSLRTRTG
                     IASQLDAIPGVGPARRRALLKHFGSIDAIRAADVDALAAVPGIPRDVAVAIKENL"
     gene            complement(24531..24974)
                     /locus_tag="AC162G_12350"
     CDS             complement(24531..24974)
                     /locus_tag="AC162G_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076656.1"
                     /translation="MQSGQQFKLDQPGLIKAGPVALVVAAVSGALFIIFALWSVASLL
                     GWIVPIFAGVWYVMTVRKGGSLPEQMDALVNGAVLGAVAGIVFGIVALITAPIGLNSA
                     TGGFGGLFVSAFGFGNLITEVIFGAIGGAAGAFGYWYLVKQGTIK"
     gene            complement(25141..25959)
                     /locus_tag="AC162G_12355"
     CDS             complement(25141..25959)
                     /locus_tag="AC162G_12355"
                     /inference="COORDINATES: protein motif:HMM:NF021384.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HIR5076657.1"
                     /translation="MRLFTRRLRPARRGRAASFPATLWQSLIVGLALSVIALFACAGP
                     HDALSKADMIGFATCHRIPERSLHIDGHQLPLCARCTGIYIGMLAGWLWLIARRRTHA
                     AQLPARPILVALLGFVALMGIDGLNSAAMLIGLPHPYETENWMRVFTGSLYGLAISLL
                     VTPLITVMLWREPTGERTLKGWGELLLVVNITSVMVMVALTEASFLLWPLVAVSVVGV
                     VGLMTLMNLSIVATLAGKANTYVGWRDLIAPGLVGISLTLVEFTGIGLLRAGLL"
     gene            complement(25995..26432)
                     /locus_tag="AC162G_12360"
     CDS             complement(25995..26432)
                     /locus_tag="AC162G_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076658.1"
                     /translation="MERCPKGIAAVKVGNLSLARQLLDAALAEDPTDVQAWLWMSGIV
                     HTPAEKRQCLRRVLEIDPANAAAKRGLEALGPEPAAAFVADSARPQVSEPVHAMTVER
                     KDDTAPRRATASPDRWSNLLTVALFIAVILLLILILALLANRV"
     gene            26476..28032
                     /locus_tag="AC162G_12365"
     CDS             26476..28032
                     /locus_tag="AC162G_12365"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5076659.1"
                     /translation="MMPAPPSSPHTRPVIVVGAGMAGLSAALSLAERGIPPLVLEANP
                     RFAGGRVAGGPVTEFEHAGETWQFRSEHGIHGFWSEYRNLLALLGRNNILPRFNPAVE
                     EEWILARGGVVRRAAAGSAIRNSPFPAPFHYAMMFLRPRFWSMLDLRDWLSIPVVVGR
                     LFGAMSIDPLREGKSLQGLTLEDFCRGWSPSLRAFFAGLARSGLSAHPDEVPLDGFIA
                     FLRFYTLLRRDAWRFDYLPADAGTALVEPMLARLQHLGGEVILGARVNRLQRAAGGWR
                     LRYDFDRSTIYSESRHVILATDAPAAQRILRDSPDTADLADALAFPTGTATAIVRLWF
                     DASPRFGPEAGIFTGDFVLDNFFWLHRFQTDFAIWHAATGGSAVEAHVYGPPEVIEQM
                     DAVLLAQMITDVFHAWPELRGHLIHHILQRNAATHTLFKIGSGDDRLGTHTPWTGIAA
                     CGDWVRHPTPALFLERACVTGIDAANLVLEALGKPPWPLASHAGPEWMAGLLERFFRG
                     TRMALHKTKR"
     gene            28148..31804
                     /locus_tag="AC162G_12370"
     CDS             28148..31804
                     /locus_tag="AC162G_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5076660.1"
                     /translation="MDDLIGRTLGQYEILQLLGKGGMASVYKAYQASLQRYVAIKILP
                     PHLAQNPEFSKRFAREARAIAQLDHPNIVPVYDYGQAEGFTFIVMKYVEGGTLKDMLE
                     RRGRLPLDRAALILGQVASALDYAHGQGVIHRDIKPANVLMNRPDWALLSDFGLVHIA
                     EGSVKLTGSGVSLGTPEYMSPEQAQGLEIDARSDIYSLGVMLYAMTTGQAPYTGNTPI
                     AVILKHVNEPLPSPRDSNPDLPVAAESAIRKALAKHPEERPSSAGELSRSFSTAIGEK
                     HPVSPALSVVEGDSEPQPRVSATPPTPKTEVAAPQAAAPLSPRRKQSLLLIAGAAIIA
                     IAALGIGVLLGGRSGAPAPAPPSPPAVESTVTPAPAAAGALQLPIRSTVQIWVLQKAG
                     DPPHTAGEGREFQYAWSGSGSIVTPDGLILTNAHVVLPDKVYGTVDGLEIWLTFQEDA
                     APEPTFRAQTVVADPDLDLAVVKIISDLDGNPVDPDALNLPAVELGDSDELSMGAEVF
                     ILGYPGIGGENITLTNGRVAGFTNAPGVKGRAFVKTDATIAGGNSGGLAADAGGHLIG
                     VPTQLGYGGEDQYVDCRVLADTNGDGAIDERDGCVPTGGFINALRPINLALPLIRQAQ
                     AGIVSLPTVEPEREPIPTPANAGEVLFSDDFSSSDSGWSEDEDEDIAARYEDGHMAID
                     VFTQNYVGWTNPDQSFGDVDVEVDTQYLGGPLDNQIGLIVRYQDADNFYVFSISSDGF
                     YEFSKLVDGEWITMLPWSTSDLIDQDSGQNRIAVVAEGDRFTFYINGVQVDQAFDDEF
                     GDGDIGLLAGAFGEGGVSVGFDNLLARVPGGSHEVSVDVSPTADVQMILSDSFDDESG
                     GWYVGSSKDVLRTIADGELTIEVAAESYAGWSDLEGVELPDVVVELEARKVSGPDLNA
                     FGLLCRYQDSENYYALEIGSDSTYTIYRVASGEYASLVDWTSSEAIAPGTDANQLRVE
                     CIGDHLRLFANGVLLADVVDPAGRGASSDALAGGSVALIVETFDDPGVLVGFDNVVVG
                     VPVAGGGAEVLFSDDFSSPDSGWDRASDKDSLSDYVDGQYVIGISAESLYVWSNPGQS
                     FTDVVIDVDATQVAGPDNNDYGVLCRYQDFDNFYRILISGDGYYAFIRQVDGESTQLI
                     DWTQSSAINQGAAHNHLTVICDGDSLALWVNDKFVAEASDGALGEGDIGLTAGTFDEA
                     GVRVAFDNVSVTAP"
     gene            31815..>32310
                     /locus_tag="AC162G_12375"
     CDS             31815..>32310
                     /locus_tag="AC162G_12375"
                     /inference="COORDINATES: protein motif:HMM:NF051939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HIR5076661.1"
                     /translation="MFVPSLLLKKLYTTGSLKNTERGVQFSIKNRLSDAELVAVRGIA
                     INGTPVPLQSLSLDLGDGRMLSPDHISADRPLAFPLRTVVTIQAGVEPLSLGKHEIEI
                     AFEAKPFGKLKFSVEDAIAAPEEGRVRIPRDPADDYSDEIVRVRQAFVEQFTGVKLHH
                     VSHYS"
BASE COUNT         6045 a        10394 c         9970 g         5901 t
ORIGIN      
        1 gcgcgcggcg gcgacgtgct ggcgcccggc cggcccgacc ggcaaatcca gttcatcgat
       61 gtgcgcgacc tggcggagtg gatgatccgt gccgttgagc aaaacctcac cggcgtgtac
      121 aatgccaacg ggccggacga ccacttgagc atggggacac tactggagac gagcaaagtc
      181 gtcagcggca gcaacgccca cttcgtctgg gttgaagacc aattcctgat cgattcgggc
      241 gttcaaccat ggagcgaagt gccgctgtgg atacccgaat ccgatcccga tgcggcgggc
      301 ttcttcgcgt tcgattgccg caaggccata gccgccgggc tgaccttccg cccgcttgaa
      361 gagacgatcc gcgcgacgct cgaatgggag acgactcgat caacggagta cgagtggcgc
      421 gcggggctga agccagaacg ggaggcggag ttgatcggga tatggagtga tcacgtgatc
      481 acgtgattac gtgattacgt gctgattgaa cctttcaaga tcgatcggcg tatcttagtc
      541 gaggagcatc cggccaggca tttcctggtg taagacgtgc ggagcactgc gccgtgaaag
      601 ttgcagcgaa cgccgattaa atttatacta tgtgtcgatc cggccatgca cgcccgcgca
      661 tggcggttac tacccgtgac catacaggtc aacacaaaaa ggaggagtca catgtctgcc
      721 cgtaagtggt gcttgacgct cgtgctggcg ctggcattga tagcgtccgc ctgtgggccg
      781 gcagcaacgc ccacgccgac tgaaccgccg gcggcgccgg cgacccagcc gcctgcgccg
      841 cccacggaac cgccgccgac gccgaccgaa ccgccggcgc gcaaagtggc tacgttcatc
      901 tggacacagg aattcgacac gctgaacccg tactacacca acatgtggtt ctccaccatt
      961 acccatcagt tctggaactg ctggccgtgg atcttcgacg atcaaaacaa cctcgtgccg
     1021 gtgctcgtcg aggagctgcc cagcacggaa aacggcggca tctcggccga tggcaagacc
     1081 ctcaccatga aactgcggga tgacatcact tggtcggacg gtacgcccat tacggcggac
     1141 gacttcgtct tcacgtacga gatggtaatc aacccgaaga acatcgtcgc ctccaccgac
     1201 ccgtacgaca agatggagtc cgtgaccgcg cccgacccgc agaccgtagt ggtcacgttc
     1261 aaggatgcat acgcgccgtg ggccggctcg ctgtggcatg gcatcctgcc caagcacgtc
     1321 ctggagccgg tccttgaaaa ggacggcacg ctcgacaacg ccgaatggaa ccgcaatccg
     1381 accgtcggct gcggtcccta caacttcgcc gagtgggaat caggctcctt cgcgcgcttc
     1441 gtcgcccgcg acgcctactg ggccggccgg ccgaagatcg acgagatctt catccgcttc
     1501 gtgcccgacg atgcgtcgca ggtcgccgcg ctcaaggccg gtgatggcga tctgggcacc
     1561 ttcatcgcct actcggacgt gccggacctg gaagcggccg gcgtggcgat gtacaaagcc
     1621 ttctccggtt acaacgaggg catctacttc aacctcagtt ccgagaaggg tcatcccgct
     1681 ctacaggatc agagggtgcg ccaggcgatt gcgctggcca ccgaccgtga gtcgttgaac
     1741 aaggacctgc tgctgggcct gacgcagccg gcggcgaccg actgggataa cacgccctac
     1801 atcgatccgt cgatcgatcc ctggccctat gaccccgagc aggcaaagca gttgctcgac
     1861 gaggccggct gggtggacag caacggcgac ggcgtgcgcg acaaggacgg cgtcgagctt
     1921 gtgctgaaat acggcaccac cacgcgcgag atccgcatgg acacgcaggc cgtgctgcag
     1981 cagcagttgg ccgaagtcgg catcaaggtc gagctgctca acttcgaatc ggacatctac
     2041 ttcggcggct acgccgatgg cggcccggcg gcaaccggtc agctcgacat cttcgagtac
     2101 tccaattcgc cgcagttccc cgatccagac acggctgaat ggctgtgcag cgagatcccg
     2161 accgacgagt acccggacgg cacgaactgg tcactctact gcgacgagga gctggacgcg
     2221 ctgttccaga agcaggccac gcaggttgac ttcgccgagc gccaggcaac cttctatcag
     2281 atcaccaaga tgatcttcga ccaggtctac tggctgggcc tgtggcaaga tccggacatc
     2341 tgggccgtca attcccggtt gaagaacgtg aagttctccg gcaccacgcc gttcttcaac
     2401 atcatcgagt gggatatcga gtaacagtcc gcctgcaacc gcaacccatg gccgggacgg
     2461 gtgcagggcc tgtcccggct cccgcaacga gaaacccggc ttccatgaga agccgggttt
     2521 ctaaagggag gatgtatgac ccgctatatc atccgccgct tgctgcaggc cattcccctg
     2581 ctgttcatcg tcagcgtcgt catcttcgtt ttgatggaca gcgcggccga tccgctggcg
     2641 acgatgggcg ggcgcaaggt gacgcgcgcc gaagatcgcg cccgcctgac gcggcagctc
     2701 ggcctcgacc agccgctgca catgcagtac ctgttctggc tgatcggcaa cgactggacg
     2761 aagaccgatc tcgatggcga cggcgtggcc gagacgcccg gcacgcgcaa gggcatgctg
     2821 cgcggcgact tcggtacctc gctggtcgag cgccggcccg cgactgccgt catcctggat
     2881 cgactgccga acacgttgct gctcatgctt gcggcagagg tggtgatcat ctgcttcgcg
     2941 ctgatcatcg gcacctactc agcgatgcat cagtactccc tgacggacaa cacgatcacc
     3001 gctctgtcgt tcatcttcta ctcgatgccg atcttcttca ttgcgctagg ttcgatttac
     3061 gtgttcgcgg tcaacttcga gaagtggggg ctgccgtacc tgcccaccaa cggcatgttc
     3121 gatccgcaag tcggcgagac gccgcagcag gtggcctggc acatggtgct gccggtgctg
     3181 agcattgcgc tgatcagcat ggccgcctac agccgctaca tccgcagtgc gatgctcgaa
     3241 gtgatgaatc aggattacat ccgcacggcc aagtcgaagg gcctggcgcg gcgctacatc
     3301 atctgggtgc atgccttgaa gaacgcctcg ctgccgatcg tgaccctggt cgggctcgat
     3361 ctgccgttac tgctggccgg ggcgctggtc acggagcgca tttttgcctg gccaggcatg
     3421 ggccgcctgt ttctggatca cgtcgagcgc ggcgacgtct ccgtggtgat gggcatcctc
     3481 atgctgatca cgttggccgt ggtggtgttc cagatcatca ccgacatcgt atacgcgtgg
     3541 ctcgatccgc gcattcggta tgcgtagtgt tacccgcaag aggtccgcta tgagcacagc
     3601 cattgaagcc ggccgcattc ctcgtcgctc tgccgacgag atcgagtcgc cgccactcac
     3661 actccgccaa ctggccgggc ggcgctttcg ccgccacaaa atggcgatgt tcggcagcgt
     3721 catgctcatc gtgctgattc tctggtcact ggggggcgcg cttttctata ccgaggcata
     3781 cgccaactac aacgacacgt cgctacgcct gcaagatccc tcggctgagc atcccttcgg
     3841 cacggacacg atcgggcgag acattttggc ccgcacgatc tacggcgggc agatctcact
     3901 gctgatcggc atcacggcgg tcatcatcga ggttatcgtc ggtgtgctga tcggcgcgat
     3961 cgctggctac tacggcggcc gggccgatgc cgtgctgatg cgcttgaccg aggcgatgct
     4021 gtgcatccca cagatcttcc tgctgatcgt gatggccaag ttcttcggcg gcaagatccc
     4081 caatgtaacc atcctgggcc gagagttcag tgggagcgta gtggtcatcg tcctgatcat
     4141 cgggctgacc agttggccgt acctggcgcg catcgtccgc gccgagttcc tggccctcaa
     4201 agagcgcgag ttcacgctgg ccgcgcgggc caccggcaca cagccgcgct cgatcatttt
     4261 ccgccatatc ctgcctaact gcgctgcgcc gattattgtc tcggccacgc tcggtgtggc
     4321 gaacgccatc accaccgagg cgtacatcag cttcctgggc ctaggcgtgc ggcccccgac
     4381 ggcgacatgg ggcaacatgt tggagagttc gtacaactac atcgagtcgg cgccgtggct
     4441 gtggttcttt cccggcatgt tgatcttgtt gacggtgttg agcatcaact tcgtcggcga
     4501 cggcctgcgc gacgcgctcg atccacgtag tcgagttgtc tgatcgtcag atggtctgtt
     4561 agtcagatag tctgatagtc acgtggtcgc atagtgctgt ggctggatga ccggccatgc
     4621 gactcgttgg ctgtctggct aattgactac acgactaatc gaccacacga ctaaccgact
     4681 aagcgactat atgactactc tcctcgaagt gagcgacttg cggacacgct tccacacgcc
     4741 ggaaggcaca gtgtacgcgg tcaacggcgt ctcattcgac ctgagcgaag gcgagacatt
     4801 agcggtggtg ggcgagagcg gctgcggcaa gtcggtgaca atgctgtcca tcctggggct
     4861 gatcccgaag ccgcccggcg agatcgtcac cggcagcgcg ctgtaccgcg gtgtcgattt
     4921 gttcaaggtc acggagcagc agcttgagca catccgcggc aaagacatcg ccatgatctt
     4981 ccaggacccg atgacgtcgc tgaatccggt gctcaccatc gggcggcaga tcacggagtc
     5041 gctgcggatg cacatgggca tgagcgcaga tcaggctcgc gagcggacga tccagttgct
     5101 gggaatggtc ggcctctctg atccggtgcg gcgactgggc gactatccac accagttctc
     5161 aggcggcatg cgccaacgcg tgatgatcgc tatggcgctg gcctgcgcgc ccagtatcct
     5221 catcgcggac gagccgacca ccgccctcga cgtcacgatt caggcgcaga tcgtcgaact
     5281 ggccatccgc atgcgcgagc agatgggcat ggccctgatc tggatcacgc acgacctggg
     5341 cgtggtggcc agcatcgccg atcgggtcat cgtcatgtac gccggctaca tcgtggaaga
     5401 agccggtatc gacgcgatgt acgacgatcc gcgccacccc tacacgctgg cgctgcgggc
     5461 ggccctgccg cgacacgatc gccgccgcga tcatcgcctc aaatcgatcc ccggcgcgcc
     5521 acccagtctg caagtcaagc cgcacggctg ccctttcgcg ccccgctgcg aattcgcgct
     5581 cgagcgctgt tgggaggaga acccgacgct cacgcctgtg gatcagaatc atcgcatcgc
     5641 atgctgggtg gatgtgacga cgggagcacc gcgatgagca cagccgagcc gctggtcgtc
     5701 gtcgagaacc tcaagaagca cttcccggtc atgcgaggaa tcgtgtttca gcgtcaggct
     5761 ggcgcggtgc gggccgtgga cgacatctcg ttcactatcg cgcgcggcga gacgcttggc
     5821 ctcgtcggcg agtcgggctg cggcaagacc acggccgggc gtacactgct gaacctgtac
     5881 ccgcccacca gcggcaaagt cacgatcgac gggatggatg tgtacgcggc aaagggtcgc
     5941 gaccaaatga tcatccgccg caagtcgcag attatcttcc aagacccgtt cgcatcgctc
     6001 aatccccgct ggacggtcaa cgcaatcgtc ggcgagccgc tgcgcgtgca caagctgcgc
     6061 cgcaccggca cagagcgcac cgagcgcgtg aaggagctcc tggaactcgt cgggttgagc
     6121 gcgcgccacc tcaaccgctt tccgcacgag ttctccggcg gccagaggca gcgtatcggc
     6181 gtcgcccgcg cgctcgcctc cgagccgctg ttcatcgtct gcgacgagcc gatctcggcg
     6241 ctcgacgtgt cgatccaggc gcaagtggtc aatttgctcg aggatcttca ggataggctc
     6301 ggcctgacgt atctcttcat cgcgcacgac ttgagcatgg tgcgccacat ttgcgatcgg
     6361 gtggctgtga tgtatctggg catgatcgtc gagctggccc cgcgcgacga cctgtacgag
     6421 aatccgctgc atccgtacac gcaatcgctg ctctcggccg tgcccattcc cgaccccaag
     6481 aaggagcgcg cccgcaagcg catcatcctg accggcgacg tgcccagccc gatcgatcca
     6541 ccgtccggct gccgcttcca tccgcgctgc ccgattgcaa aggaccattg ctcgaagatc
     6601 gtgccggagt ggcgtgaaat caaatcgggg cattgggtcg cctgccacga ggcgcggctg
     6661 actgagtaga tagcctttca cttcacggca gaggcgcagc gacgcgacca gcgtcatgcg
     6721 gttgcgccga aacaaaatag ccatatacta tgcaccgaac gacacacgtc gtcccacgct
     6781 gctttccgtt cagacgcaat cggtatccgc cttgaccccc gcaagggtca cccgaatctc
     6841 attcaggagg agacgagatg tggaaacgtg cttggtatct gacgtcgctg ctcttggtgg
     6901 cggcgctggt cgggtccgcc tgcggtggcg ccgcgacgac gccgcccgcg ccgaccaccg
     6961 cgccgccggc tgagactcag ccgccaccgc ccaccgagcc gccgcccgca acacaagagc
     7021 cggccatcct gcgcatcgga tggtccggcg cgccggactc gctcaacccg gcggccggcc
     7081 tgctcaccga atcgtacacg ctgtacgagc tgacgtacga cacgctgatc ggcctcgatt
     7141 tgagcggcac gtattatccg gaattggctg agagctggga ggtgtcggac gatggcacgg
     7201 tgtggacttt ccacctgcgt gagggtgtga agttccacga cggcaccgat ttcaccgccg
     7261 aggacatggc cttcagcatc cagttctaca aggatcacga ggagttcccg tatctcaaca
     7321 gttacacgct caacttcgag acggtcgaag ccaccgacga tgccacgttg gtcatcactt
     7381 tgggcgaacc gatcggcaac atggaatccc aggtgctgtt cctgtacgcg ctgcccaagc
     7441 acatctggga aggtatcggc ttcgacgagg cgttgacgtt tgagaatttg gagatgatcg
     7501 gcaccggccc gttcaaagtg gtcaagtacg tgcgcgacca gtcgatcgag ctggcggcca
     7561 acaaagatca ctaccgcaat ccgcccaaga tcgacggggt gatcttccaa acctacgaga
     7621 gctcggatgc gctggtgcag gcgctgcgcg aaggccaggc cgatctgatc accgacgtgc
     7681 cgccgacgac gatcgcctcg ctgaagcagg aggccaatat caccgcgcag gtgggcggca
     7741 gccgcagcct gcgcgacatc atcttgaatg tcgtgccgga cgacaagtgt ccggctgaag
     7801 gcggcgtgtg ctccggccat ccggccctca aggacagggt agtgcggcag gcgctagcgc
     7861 acgccaccga caagcagcag atcatcgacg tggccttgct cggcctaggc acgcccggcc
     7921 tgagcatggt gccgcgctcg ctgggcgact ggttcaacag cgaactcgaa gactacgcct
     7981 acgatgtcga tctggcgaac aagatgctgg acgacgccgg ctacaaggac accgatggcg
     8041 atggcgtgcg cgagatgcct gacggaacca atccgctcaa attccggttc ttgatcccct
     8101 ccgacatttt gaccggcgcg cgcgaaggcg acttgatcaa agcgatgtgg aacgaagcag
     8161 gtgtagacat cgacatccag gtgttggaac ccgacaccgt cacggcggct tgctgcccgg
     8221 ccttcgacta cgacgtgctc ctgtggggct ggagtgccga tcctgatccg gcctacctgc
     8281 taggcgtgct gaccaccgac gagatcgaca ctggtttctc cgagacgggt tactccaacc
     8341 cggtctacga tcagttgtac ctggatcagt cgacggcggt cgatcacgag gcgcgcaaga
     8401 agatcatctg ggagatgcag aagatcgcgc tcgaggatgt gccgtatatc attccgtatt
     8461 accagaacaa tgtgatggct taccgcaacg acaagttcac cggttacgtt tacgtagagg
     8521 gcggcctgtt gtcgctggac gatccgctct cgctgtcgaa cgtggagccg gttcgataat
     8581 caatatcctc ccgcaggtta tccaacctgc gggagggtac tgttgtggag tgagtatggg
     8641 acggcgcaac tacctgctgc gcaaggcggg cgcatcgctc ctgaccatct tcatcgtcct
     8701 ggtcatcaac tttttcctgt ttcgcgtcat ccccggcgat ccggccaagg ccggcctcaa
     8761 agaccctcgg gtccggccgg aggtgatcga ggcccagcgg gtgcgcttcg gactggacaa
     8821 gccggtgatc aactgcttca aagggcagat ggtattcgac gccagccggt gcgccgtcaa
     8881 tccgctggac acccaactgt tcatctacct gggcaacctg ttcaaaggcg atctgggcat
     8941 ctcttacgat cagaagcgcc cagtcgccga catcctgcgc gagcggatcg gcaacaccgt
     9001 gatcttgctg ctgggcgcgg aacttttcgc catcatcctg ggttccttat tgggtttggt
     9061 ggccggttgg aagcgaaaga cccggctcga cacttcggcc ttgatctttt cgctgacggc
     9121 ctggtcgttg cctacgttct ggctcggtat cctgatgttt atgatcggca gtagcgcatt
     9181 cggtttgccg gtcaatggga tgcgcacgac cggcctgacg tacgctaatt tcggagaaga
     9241 actggccgac gttgcccggc actggctgct gccctcgctg acgtatgcca tcgtgctact
     9301 gggccagtac atgatcgtca tgcgcgcctc ggtgttggag gtcctctcgg aagactatat
     9361 cctcacggcc aaagccaagg gcctgacgaa ctttcaaatt ctgaaagacc acgcgctgaa
     9421 gaatgccatg ctgccgatgg tgacgctggt gctgttgaac ctgggcttta cggtctcggg
     9481 cgcggtcgag ttagagcggg tcttttcgtg gccaggcgtc ggcctggcga tcgtcgaggc
     9541 ggtggcgcgc aaagattacc ccgtgctgca aggggcgttt ctgatcgtga cgatcagcgt
     9601 catcttcgcc aacctgttga tggacttgct gtacacctat ctcgatccgc gcgtgaaggc
     9661 ggagtagtcc catgaaccga ttctcgcgtt ggcgcgacgg cttcaaaaat ttctggcgag
     9721 tgttccgcca taaccgcctg ggcttactgg gcttgatcat gctggcggcg gcgattgtga
     9781 tcgccgtgtt cgcgccgctg atcgcgccgg ccgatcctta tgcccgcgtc agggtcaagg
     9841 tcgaagatat ttacgcgccg cccagcgcgg ctcacccctt cggcaccgac gacgccggca
     9901 aagacgtgct gtcggcgttc atctatggtt cgcgcgtctc gttgatcgtc gggtttgccg
     9961 cttcgctgat ctcgctgttc gtcggcggag cagtcggcct gatcgccgga ttttacggcg
    10021 ggcggctggg caacttcctg atgcggatca acgacatctt tttggtcatc ccgtttctgc
    10081 cgctggcgat tgtgctggtg gcgctgctgg gccaaagcct gctcaacatc attctcgtca
    10141 tcggcctgct cggctggagc agcaccgccc ggctggttcg ctcgcaagtg ctgtcggtca
    10201 aagagcggca attcgtcaca cgcgcgcggg cgatcggcgc cggcaatctg cacatcatct
    10261 ggcaccatat cctgccgctc gtcctgccgc tgctgctggc gaacaccgtg ctgatcatct
    10321 cgctgtcgat cctggccgag tcatcgctgg ctttcctggg gctgggcgac ccgacagtta
    10381 tcagttgggg gagcatgctc aacttcgctt tcacccgcag cgcgatgagc gcgggagcgt
    10441 ggtgggcgct ggtgctgccc ggcctgggga tcgtgtgggt ggtgctggcc tgcaccctgc
    10501 tcggcaacgt gctggaagaa atcttcaacc cacacttgaa ggcgcatcac ctgtccagca
    10561 cagacaaaat ggtagcgatt ccgaatgaaa ttcaacccgc gccgtctggg aaatgaccct
    10621 gcctcgatcg cgctcgactg gcagatcgat aatatgtcct cgcctgcgcc gttactcgag
    10681 gtcaaaaacc tgtccaccca cttcctgacc gacgacggcg taatgcacgc cgtcgaagat
    10741 gtctcgttca ccctggatcg cggccagtcc ctgggcttgg tcggcgagag cggctgcggc
    10801 aagacgacga cgatgctcgc gttgatgcga ctgctgccac ctaatggccg gattgtcaag
    10861 ggtcaagtcc tgttggaagg acgcgatctt ctgcgtctgc ccgactccga gatgcagcgg
    10921 gtgcgctgga gcaagatcag tttgatcttt caaggcgcga tgaacgcact taatccggtg
    10981 cggacagtgg gcgatcagat cgccgaatcg atccggcgcc acggcctgat gggtgggcgg
    11041 ctcgacaacc acgcgctcga tcagcgcgtc cgccaattgc tggacatggt tggcattgcc
    11101 tcgtcgcgcc aggatcaata tccacaccag tattcgggcg gcatgcgcca gcgggcaatg
    11161 atcgcgatgg ctctggcctg cacgcccgac gtggtcatcg ccgacgaacc gaccaccgcg
    11221 ctggacgtga tgatccaagc tcagatcctc gacctgctgc agaagcttca gcgcgaactc
    11281 gggctggccg tcatcctgat cacgcatgac ctagccgtgg tggccgagat gtgcgatcgg
    11341 gtgctggtaa tgtatggcgg caaggtggcc gaatatgccg acgtcgacgc catttacaat
    11401 gagccgctgc atccctatac tcgactgctg ctcgcagcct ttcccgatct gacgcagccc
    11461 gaaggcaaac tcgtggcgat cccggggtat ccgccgcgcc tgaatgcgct gccggccggc
    11521 tgccgcttcg agccgcgctg cccggtacga atcggcaagt gtgtcacggc cgatccgccg
    11581 ctgcgcgaag tcaagccggg tcattgggtc gcgtgccatt tggtcactga gcaattaccg
    11641 attcatccta ccacttcgtt gccatgaacc ccactcccgt gcttgaaata actcgtctgc
    11701 gtaagcactt cccgatctca cgtggactac tggcctcatt ccgcagacga tcggctcaca
    11761 gcgttcacgc ggtagacgat atttcgttcc aggtgcagcc gggcgaaatc ttggcgctgg
    11821 tgggcgaaag cggctgcggc aagacgacca cggccaagct attgatgggc ctggaaccac
    11881 caagcggagg cacgatccac ttctgtggcc gggatgtcgc gacgttcgat ctcaagcaga
    11941 tgcggcggca gatccagatg gtctttcaag acccgtacga gtcgctgaat ccgcgcatga
    12001 cggtgtttga gatcgtggcc gagccgctga atgtccatca cctggcgcgc agccgggaag
    12061 acaggatcga tcgggttaag cgcgcgttga aagatgccgg acttaagccg gccgagtcgt
    12121 atttcaaccg gctgccgcac gaactgtcgg gtgggcagcg acagcgcgtc gtgatcgccg
    12181 ccgccctggt gcttgagccg accgtgctgc tggccgacga gccggtatcg atgctcgacg
    12241 tgagcattcg cgctgatatc ctgaacctgc tggccgagct gcgccagacg cgcggcatct
    12301 cggtgctatt catcacccac gacctgggca cgattgccta tttcgccgac cggatcgcag
    12361 tgatgtatct gggacggatc gtcgaaatcg gcccggcgcg cgacgtgctg agcaatgcgc
    12421 agcatcccta cacgaaggcg ctgctgtcgg tcgtgcccgt cccgaatccg cgcctgcgtc
    12481 gcgagcgcat catcctacag ggtgagacac cgaacccgat cgacctgccg ggcggctgtc
    12541 gcttccatcc gcgctgcccg gtcgcgatcg agcaatgccg ccaaatcgac ccgccttatg
    12601 tggaactccc gaacagccac agagccgcct gcctcctgat cgaatctacc cagtaagggt
    12661 ccccttgcgc cacacgccct ccacggatga ctgtggggag aacgaaggaa ttggaaaatg
    12721 gttggcgcga gcatcggcta aatttgacag catgaatccc cggtggtaaa ctgagctttg
    12781 tagtcggcag caagcagcac aacggcgaat gcgcggtgta tgccagcggt cgtcgtgatg
    12841 ggcgctctgc cctacagaaa cttcgtgggg acgaaaggag tccaagctca tgagcgagcg
    12901 catcacaggc acggtgaagt ggttcagccg gacgaaaggt tacgggttca tcacccagcc
    12961 gaacggaccg gacgtcttcg tccactattc ggccattcag ggtgaaggat tccgcaacct
    13021 caacgaaggc cagcaggtgg agttcaccgt gctggaaggc cccaagggcc tgcacgccgc
    13081 tgacgtccgc gctgtgtagc ccaggacgca ggcgaattga gcctcaacag tttcctgttg
    13141 aggctcaatt tattgtatga caaaagcaaa agtcccgccc ggggggaggc gggactttca
    13201 aaccggtgag gcccacgggg gagtagagtc tcaccggtgc cgcccatggt atgtcagcat
    13261 aatcacgtaa cttggcgggg cggtgacgcc gtccggctat tgacgagttg ggactctgag
    13321 gagccggata tattatcgag tatgccagca attggtaaaa ggctcataac agaggccccg
    13381 cctggcataa cagacgcata acacttgtgc cccgaaccga ccgattcggg gcaccatgac
    13441 aactgacgca tctgagcctg cgagccatct gggagcgcgg tactatgcgg cgacgaaggc
    13501 cggctccaat ccggcttcgg cccggatggc gtcggccttg tccgtcactt cccagggcgc
    13561 ttcaatgtcg tggcgcccga agtggccata ggctgcggtc ggccgataga tggggcggcg
    13621 caggttgaga ttctggatga ttgcgcccgg ccggaaatca aagtgggcgc gaaggatgcc
    13681 caggatcttc tcctccggca ccttgccggt gccgaatgtc tcaaccatga tggacagcgg
    13741 gcgggccatt ccgatggcgt acgatacctg gatctccagc cggtcggcca ctccggcggc
    13801 caccaaattc tttgctgcat agcgagcgta gtacgccgcc gagcgatcga ccttcgtcgg
    13861 atccttgccc gaaaacgcgc cgccgccgtg gcgagccatg ccgccgtagg tgtcgacgat
    13921 gatcttgcgg ccggtcaggc cggcgtcacc catcgggccg ccgatgacga accggccggt
    13981 gggattgaca tagtatttgg ttctggcatc gagcaaatcg gcgggaatga cttcccggat
    14041 gactcgctcg atcacgtcgt cgcgaatctg tttgtgcgtc acgcgctcgt cgtgctgcgt
    14101 cgaaacgagc acggtatcca cacgcttcgg tttcccgtac gcatattcca cggtcacctg
    14161 tgacttggcg tcggggcgga gccactcgat ctcgccattc cggcgggcat ggctgaggcg
    14221 cttgacaagc cggtgagcca gggcgatggg aagaggcatc aattccggcg tttcgtcgca
    14281 ggcgaagccg atcatcatac cctggtcgcc tgcgccgacc gcctcgatct ccgaaccggt
    14341 catctcgccg ctcttggcct cggcggcccg atcgacgccg atggcgatgt cgggtgactg
    14401 gcccttgagc gatacgacca caccgcaggt gcgcccgtcg aaaccgtatg cggcgtcggt
    14461 atagccgatg tccgccaccg tctgccgcac gacgtcctga acctcgaggt gagcctgagt
    14521 ggtcacctcg cccatcacga cgatcaagcc ggtcgtcgcc gacacctcgc acgcgacgcg
    14581 cgatcgcgga tcctggctca gcatcgcatc gagaacggcg tcggagatct ggtcgcacat
    14641 cttgtcggga tggccctcgc tcaccgactc cgacgtgaag tacaactgcg gcgaccgcat
    14701 aaaggaattg gtcacgggaa ttccccctga gagtagattt ctcagacctg cggctcaaaa
    14761 aaacctgcct cttcgcgacg cagaagaggc agagtgatgg gcatcaccgt cgacctctca
    14821 tcttccagaa ctcacgtctg ccggagttgg caccgggttt ggagttagag ttagagtcga
    14881 gagttagagt ccagactcta actctaactc cgcactctaa ctccagactg gttgccgagg
    14941 cttcacaggg ccggtccctc cgcctctctg gataagagca tcgcaagtct tcgattgatt
    15001 ggcagcgagt ttaccatagg cgcacattgt tgtcaaagtg gacatcacca ggtgatatgt
    15061 gttgccaagc ctgcgagaga atgctataat ccaagccgct cgggcgcgta gctcagccgg
    15121 ttagagcaca cggttcacat ccgtggggtc acaggttcaa gtcctgtcgc gcccactcgg
    15181 cggccgcggg agcgccaagc gcaggggggg cgccgcggcc cttttgcttc cagactcgcc
    15241 cgtgctgcgg atccggatgc gctgattctt ccacttcaca gctactccgg tcgcggcgga
    15301 taacatgcgc acgatcaagc ctctctacca gatcgacgga cggctggcca atctgcgctg
    15361 gttcctcctg atcactgccg gtatcgtgat cgcgcttcaa gcccaagtga ccgggtcgga
    15421 cctgcccgct ctccagatcg ccgctgtcat cgtcgcctgt atcttcaact tgctcatcgt
    15481 cctgtcactg gccgtggaca tgtccccgcg tcctgtgccg ttgatcgggt tggcggttga
    15541 cacgatgctg gccatcatct tgatctcgtc cgatccgcgt atgacaagcc cgctcatctt
    15601 ctacgcgatc ttcccaatca tcacatcagc cttgcgcttc ggctggctct tcaacgtcgt
    15661 cacggccggc gcaatcatgg gcgcctacgt ggcgttcaag atgatcggtc gaggactggc
    15721 cggagagcag gtcctccaga cgctgggaac actcctgatc ctcggagtgg cggcgaccgt
    15781 gtcagggacg atcggtgggc gggtgaaaga cctggtgctg caggccatgc gcaccgagtc
    15841 cgaaggcgag ctgcgccagt tgcgcacccg gcgcgaacaa gcccgtgcca tcttcgagat
    15901 ggcttccacg ctgagcgcga cgctgtctta cgagcgcatc ctggaggcgg tgctcgacgt
    15961 cggcgcgatg gggcttcgcg aattcggttc cgtggcgcag cgcctcgtca gcataatcat
    16021 gctgtttacc gaagacggcc tggtggtgag cgcctcacgc cggcttacgg cgcgcgacga
    16081 agcggcccgc ctgaccgcgc cgagcggcat tatcgcacag gctctccaat cggctgagcc
    16141 tatcatttgc caggacccga agggcgaccc ggacctggcg actttcgtcg cgctgcatcg
    16201 atgcgtgacg gtcgtggtcg tgcccctgcg cgccggcttt gaatcgtacg gtgtggccgt
    16261 cttcggcagc cccgaacccg acgtgttcac gccggatcac ctggagctgc tcaccgtcgt
    16321 cgcttcgcag gctatcatcg ccctgcagaa cgcacaactt tatcagaagc ttcaagaaga
    16381 gaagaatcgc atcgtcgaca tcgaagaaga cgcgcgcaag aagctggccc gcgacctgca
    16441 cgatggaccg acgcagaacg tggccgccat cgccatgcgg ctcaattaca cgcgcacgct
    16501 gctcaagcgc agcccggcgc gcgccgaaga agaaatcgaa aagatcgagg atctcgcccg
    16561 caggacgacc aaagagatcc ggtacatgct cttcacgctg cgaccactgg tcctggagac
    16621 gcaggggctg gtgccggcgc tcgaacagta cgtgcagaag ttcaaagaga cggacgattc
    16681 ggtcgagatc atcctggagg ctggccggat caccgaccgc gttagcagag atgctcaggg
    16741 cgcgatcttc tatatcgtcg aagaggccgt cggcaacgcg cgcaagcatg cccaggcgca
    16801 gcacatctgg atcaacctcc acgaagactc ctcgcagctc accgctgagg tccgcgacga
    16861 cgggatcggt ttcgaggtgc aagccgtgca gaaatcctat gatcagcgtg gcagcctcgg
    16921 catggtcaac atgaccgagc gcgcggcgct ggtcaagggc accctctccg tcgcttctgc
    16981 gccgggggaa ggcacgcacg tcacactggt catgcccctc aagtgaggag ttcgcctgag
    17041 cgcgctcatc gcctggacgg ctggatctgt cgcattgtac gtcgttgcgg cgcgcctggt
    17101 tgcgccacgc atcggcgcgg cattgcgcgt ttcgccgcca gggcaatggc tgctggaagg
    17161 cgcgcagttc gcttacttct tgggcgtgcc atacctggcg ctgctgaccg gtgctttcgc
    17221 cccgagcgac gtcggcctgc agggatcgcc agcgctcgac ctgcttctgg gatgggcagc
    17281 agaggaatgg atgcgcgcgc tcggccaggc gattgcactg ggcttgctca cgctgctggt
    17341 cgtgcggttg ctgacatggc agatacggcg cgccggaggc tgcggggcag cggcaatgga
    17401 gatcgttgac gcgtcggccg cgcactcgat tcgcgacggg atatacgcgg aagtgcactg
    17461 gtcgttctat cgcgcgctgc cgatggtgct gtggggtgac gcaggccggg ccgcgctgct
    17521 cggaatcgcg ctcgccgccg ccgaggcgct gctagccggt cggcgcacga cctcaagcgt
    17581 gagccgcatc tccgcgtcca gcgccctgct cgggatgggc agcgcgacgt tcttcgcgct
    17641 cacaggcggc aacttctgga tcgcgagcgt actgcaagta acgatgcgag tcagcctggc
    17701 gagaatcgca cccgcccccg agcgtgccga gccgctggag gacgtcatcg tgtgagtcgc
    17761 tatactcccc tctcctgtag tcttcgacgg gagaggggtc aggggtgagg gccaccagcc
    17821 tcaaacccgc tgggcgggcg caagcagcag cggcgcgaca cctgcggccg gcaactgtgc
    17881 gcggggtcag gccatcacaa gcgagagcac gacgaggagg gcgacaatgg cggcagccag
    17941 aatcgcaata cgccgtagat cggagcgaac gtaggcatac tcctcgccca cgcctgcccc
    18001 ttcgctgcgc tcagggcggg ctctgagcgc agccgaaggg tcgaccccgg tgcgaacggc
    18061 agggatcggc gtcggcgtcg aagccggtga agcgggctgc gcaattcccg ccgtaacgtt
    18121 cgcagccgcc tgttgcacgg cgcgctgcct cgaaagttcc ttgcgttgtc gagtcgtttt
    18181 cttcgccact gagaatatcc ttgtgagagg atcacgattg cagggcagca aacacgacga
    18241 tgcgctgcgt atcgaccagg tatgggtaac acgaaatcag cgtcgccgtc ggctcgctcg
    18301 tcggatccat gacctccacc tgcgttggct cgacgatgcg cgtttcggtg acgacgtagc
    18361 ggaaagcccg atcgaccgaa tagatcatga tttcgtcgcc gactttgagc ttgtccagat
    18421 cccggaatat ctcgccgaag acatcgttgt gcccggccag caccaagttt ccgcgctcgc
    18481 ccgggttcgc cgtgttgagg tagtgcccaa cgcccttttt caactgttcc cagtcggttc
    18541 cctgcacaac attgccgtcc acccccagcg ccggaatcac aatgcgcgtc ggctgtccgg
    18601 gtccacgcgt cggaatcggg agcggcgtca ccgactgcac gtaggcgcgc aaatgagctg
    18661 gaatctcatc gaagttgggc gccgcgccgc cgggcgacgt cggaggcttg tggccgctcg
    18721 gcagcaccac ggcgcggatc actggcgtcg gctcggccga ggattgcccc acatttcgcg
    18781 caacggccac gtcgcggttc agctcctgcc gcgtctgctc gatgccgacg acgaccaaca
    18841 ccaggccaat cagcgcgccg atctcgatca ggagaaggaa tcgatcgcgc acggcacgcc
    18901 agggaaagac ccgtgagcgc cgggtctccg aaggcgatgc gatctcggcc gtgcgatagc
    18961 gggatccgat cgggtcggcc ggcgcctcgc cggcgctcgt cgccggccgg ctgagcacct
    19021 cgttcgcgaa ctcgccgtcg acgatgcggc cttcaccctg cagccggcgc agcgtggctt
    19081 cgcgcgcctg gcgcttccta attgcgagga tctgctcgag ctcctctatg ctcagatcgt
    19141 ctacgctgcg tcgatcaggc atacgaacga tgtcccactt ctataccggc gcacattccc
    19201 tacttggtac cgaccgcaaa gacctcgcgc tcgaagaacc agcgaaacgg cggtaagcca
    19261 aacgccaccc tacgcaagag gcgaaatatc cggctctcct gccggttctg cggcgggctg
    19321 tcgagccaaa ctctgacgtg ggcaaatccg gcctgcttga gattgcggtg caggctgcgc
    19381 atgtcctgct cgttgacgtg gacgtcgacg ttggcggcga cgttcagcgc gcgcgggttc
    19441 gccgggtact gcgtgctcct gccctgcatc gtccgcacca gccgcacgac cgggtacgcg
    19501 tatcggtcgt accaccggtt cggcgcagta tgaatggcaa tcttgcctcc cggcttgagc
    19561 acgcgccgcg cctctctcag cgctgcatcc agctcccacg ggtacaaatg ctcgacgacg
    19621 tcgaaaatga gcacgcggtc gaacgcggca tcaggatagg gcaagaactt ggcgtcggct
    19681 cgatacacgc cgatcgccgg ccgcccggtg gactcgactt ccgtgctggc cacgtcatgg
    19741 ctcaggcggg cggcgacatc ggcgtaatcg atgccgtagg cgcgcgcgcc cagccgggca
    19801 caatgccgca gaatctcacc ccggccacag cccacgtcca gcacgcgcat ccccggctcg
    19861 accgaggcta cggccaaagc ggcgttcagg cggcgcgaca ggtgctcgcc gtggctctcg
    19921 acgaattcct cgtagccctc acacgccgtc aggaaataat cttccgtata cagcgtcgaa
    19981 ggcaatgacg cttgagtttg ctcggacgac aagccaaact cccgacccgt gacgaataga
    20041 caaaggacgc aagacgatag ccttcggcat gggtctctcg cctttcgtca gattataccg
    20101 tgcccgatcc ggtcaggcaa cccggcggcg gatcaaccct ccccagtgag tcatttcgcc
    20161 ggcgtgcaga accgccatca cgcccaggta gacagccgcg ccggccagcg cggcgccggc
    20221 tacggtgatg acttcggcga cgaatccggg atatgcctcc agatactggc tgctccccag
    20281 cagcgcaacc acaatccacg tggcgatgcc catcgccgcc gatccgacca gcgccttgag
    20341 tgtcagcgcg cgcagcccaa atccgcgcag cccgccgatg cgccgctcca gcagccacag
    20401 cataatcaat gcatgacaga tccactgcac ggaattggcg aacgccagat ccaccacctc
    20461 gagcggccgg ccgagtatca cgcccggcgc gagagcggca gccaggtaaa tgacgacgtt
    20521 gcaaaccagc ccgacgagtg ccggcgtgag cgtatccttg cgcgcgtaaa aggcgaacac
    20581 gagcagtaga tcgaccgcgg cgaaggccag gcccggcacg tagaattgaa gcacgcgcgt
    20641 caccgcgtcg gtgtctgatg tcgcaaactg gccgtgctcg aacagcagtg cgacgatcgg
    20701 ccgcgcgagc accagcagcc cgaccgccgc cggaacgatc agcaccatca ccagacgcag
    20761 gccgcgcgcc agcgtcgcct tgaaacttgc ctcgcctccg gtattgtcga gcgaagcctg
    20821 ccgcgagagt gtcggcaaaa tcgcaatcga aacggcggtg gccaccaggc cgagtgggaa
    20881 ctggatcagg gtggtggcgt attgcatcca ggcgatgcct tcttcgcttg cgcgcgaagc
    20941 caggttgtac gacagaacga tcgccaactg gttgatcgcc agccccagca cgactggcag
    21001 atacaatctc ccgatctggc gcaggcccgg atgccgcaga tcccaggcgg gccgcaagcg
    21061 cgcatcgcgc aggctgggca attggaatac gacctgcagg atcgcgccgg caagcaaccc
    21121 gatggccatg ctcgctatgc cgaagcgcga tcccaacacc agagcgcaga cgacgatcgc
    21181 cgcgttgaag acggcgccgg tgaaggcggg cagcgtgaat cgcttgagcg cctggagcag
    21241 gccgctgagg atcgccgaca agctgatgaa gatggtggca actgccgtga cattgagcag
    21301 ggacgacgtc tgagccagca gacgcgcatc caaaccgccg ctgatcagcc gggcgacgaa
    21361 cggcgcggcg acgatgacga cgaggcacag cgccgtcagc acgacgaagg ccagtgtcat
    21421 caccagcgag gccagccgcc acagcgcgtt tctgtccctc tgcgcaacct cgctgaaggc
    21481 aggcaccagc gccgacgaca ccatgccgcc gatcagcaag tcgaacagga gcgtggggac
    21541 gagcgcggcg gcctggtacg cgctcaccag cccggttgag ccgaacagat tggccttcac
    21601 cgcttcccgg gccaggccca agacacggct ggcgacgttg cccagtgcga tgatcgacgc
    21661 agcccgggcg atgaccagtg tttcgctggc tgcttcgggc gtgatgggga tttcgctcgt
    21721 ctcgaggagt tcgctcatgc ctgcaatcaa agcacagggc ggccgatcgc cgcccacagc
    21781 cattctacca cacgccgcct acaactccga cacccggtag cgcgccgggc gatcatctgg
    21841 ccgccacgcc gacgctgact ccgtgaatag ggcgtcggcc cagccgggtt tcggcgcggc
    21901 ctgcaatccg gttatttgtc gcgccgggta gaacgtctgc gcggcgcgtc ttcttgggtt
    21961 gacgcgggta cttcctctgc cacctgcaca tccggaccga tcaagtcccc agcggcgaca
    22021 atcaagacca cgacgctttg aaggggtatc accgtatcca gcgcaatccc gctcgctacc
    22081 ccaaaccatg tcggcgctgt gatcgccagt acgaggatca tatgtcgcac gagtgtctgc
    22141 ttcatgacct accccaccac ccctgactcg ttctgtgttc gaaagtggcg gccattctag
    22201 cacgcatcgt agaactccgg gtgatcaggg ggtgcatcgg ttcacaccgc ctgttcacgc
    22261 catcccgcat actgctccgc attgccattc tgtgcgcgct ggtcctcgtc accttcccgg
    22321 tcgtggcaat gcccggcatg tcaccggccg cggatgcccg cccgccgtct ctcgctgagc
    22381 ctggcacggt gcctgcctcc actgtgatac cacagcctgc ggcctggact caactcgcgc
    22441 cggatattcg ggccaaggtc gatccgcgca tttggaattg gatttgtttc agagattctc
    22501 tttgatggcg accgccacgt cacggggaat gcccggcaca gcggcgaggg catcgacgtc
    22561 ggcggcgcgg atcgcgtcga tcgacccgaa gtgcttgagc agcgccctgc gccgcgccgg
    22621 gccgacaccg gggatggcgt cgagctgtga ggcgataccc gtcctcgtgc gaagcgaacg
    22681 gtgcgcggta atcgcgaagc ggtgcgcctc gtcgcggata cgctgcatca aaaacagacc
    22741 ctgcgagcgg cgcggcagca gaatcgaatc ggggtgtcca ggcaggaaga tctcctcctg
    22801 ctgcttggcc agcccgcaca cagggatctg cccgaacagg tcgaactcct ttaagacttc
    22861 aaccgctaca cccaactgcc ctttgccgcc gtcgacgatc agcagatcgg gcaagacgga
    22921 gaaactctcg tctttcttct ttccgggcgc cggctggctt tcctgcgctt tcgcgtcgaa
    22981 atagcgccgc aggcggcgcg tgagcacttc gcgcatcgac gcgaaatcgt ccgggccgtc
    23041 cacgctcttg atgttgaact tgcggtagtg gctcttacgc ggcacgcctt tgacgaacac
    23101 gaccatcgac gccacggtat tcgtgccttg tgtattgctg acgtcgaagc actcgatacg
    23161 cgcgggcagc gcttccagcc cgatgccgtc ggccagctcc tgcagcgcct gctcgttttt
    23221 atgctcgtcg gccatccact gcgcttgcag cgcgcttaac gtctccgccg cgttctcggc
    23281 agccatcgcc accaggtcgg ccggctggcc ctcgcgcggc acgtcgagga gcacctgcgc
    23341 gccgcgtttc tgccgcagcc actgctgaat gatctgcgcc tcttcgacct caacctgcag
    23401 cagaatttcg ggcggcaccg tcgccgcctc ctcgtagaat tgcttgagga agctcgacac
    23461 gatctcgcgc gtatcctcgc cttccgcgcc ttcgaggatg aagtactcgc gcccgatcag
    23521 tttgccctgc cggatgaaga acacctgcac acaggcgtcg ccgcgcttct cgtcgcgggc
    23581 gaaggcgacc acatcctggt ctttccccat cgtcgacacg actttctgcc gctcggcgat
    23641 gtgccggatg gcgcgcagtt gatcgcggta ctcggcggcg cgctcgaact gcattccctc
    23701 gctggccgcc tgcatcttct tctcgatctc ggccaccacc tggtcggtct tgccttcgag
    23761 gaaggcgcac aaccggtcga tcgtcgcgcg gtactcctcc ttgctcaccg cgccaataca
    23821 cggcccgccg cacagcttga tgtcgtagta caggcaagcg cgcggatcct tgccggtgat
    23881 gatgcggtcg cacgtcaggt aagggaaaat ccgcctcagc gtttcgagcg tggcgtacac
    23941 tgcgcccgcg ttggtgtacg ggccgaagta gcgcgccccg tcgcgctcca tcttgcgcac
    24001 ggtcagcact ttcgggaaat cgttctgcca ctcgaccttg acgtacggat agcgcttgtc
    24061 gtctttgagc aggatgttga agcgaggccg gtgcttcttg atcaggttgg cttccagcag
    24121 cagcgcctca agctcggtct cggtgatgat ccattccagg tcggcgatct ccatcaccag
    24181 ccggcccgtc ttgctaaacg gatcgatcga gttgtggaag tacgaccgga cgcggttgcg
    24241 caggttgatc gccttgccga catagaggat cgcgcccctg gcgtcgcggt acaggtagac
    24301 gccggacctg gacggcaccg ttttcagttt ttcggcgact tgatcgcttg gcattattcg
    24361 cccaaccatc tggacggcta caagaattct atcacgcggg atagagtagg tcaacgaacc
    24421 agagcggagc gctgcgcgca gcggcctgta ctatgaaggg gaattacaac agcccgaccc
    24481 tcggcaacga ggtcgggctg tagctggacc ctgaagcggt cgagcacggc ttacttgatc
    24541 gtgccttgct tgaccagata ccagtatcca aaggcgccag cagcgccgcc gatggcgccg
    24601 aagatcactt cggtaatcaa gttaccgaac ccgaacgcgc tcacaaacag gccgccgaag
    24661 ccccctgtgg ccgagttcag gccgatcggc gccgtgatga gcgctacaat gccgaaaacg
    24721 atgccagcca cggcgccaag gaccgcgccg ttcaccagcg catccatctg ctcgggcagc
    24781 gaaccaccct tgcggacggt catcacatac cagacgccgg cgaagatcgg cacgatccag
    24841 ccaagcaggc tggcgacgct ccacagcgcg aagatgatga acagcgcgcc cgacacagct
    24901 gcgacgacga gcgccaccgg ccctgccttt atgagtcccg gctgatcgag cttgaactgc
    24961 tgtccactct gcatgataac tcctcctgtg ttgagattag aataaatcta ttgtagcaac
    25021 gctggtcaac tggcgcaagt gagttcaacc tctcctcaga agaaacacca ccggacgcgg
    25081 cgagttgctc cgaccgtcaa accgtaaccg attctatagt cagcggctgc ctgccgccca
    25141 ctacagaagc ccggcccgca acaggccgat ccccgtaaac tcgacaagcg tcagcgaaat
    25201 cccgaccaaa cccggtgcaa tcagatcgcg ccagcccaca taggtgttcg cctttcccgc
    25261 cagtgtggcc acgatcgaca agttcatcag cgtcatcagg cctaccacac cgactaccga
    25321 taccgctacc agcggccaca gcaggaacga cgcctcggtc agcgcgacca tcaccatcac
    25381 cgaggtgatg ttgaccacca gcagcaactc gccccaccct ttcagcgtgc gctcgccggt
    25441 cggctcgcgc catagcatca ccgtgatcaa cggggtgacc agcaggctga tggcgaggcc
    25501 gtacagcgac ccggtgaaga cgcgcatcca gttctccgtt tcataaggat gcggcagccc
    25561 gatcagcatc gccgccgaat tcaggccgtc gatgcccatc agggcgacga aaccgagcag
    25621 cgcgacgagg atgggacgcg ccgggagctg cgcggcgtgc gtccgccgcc gggcgatcag
    25681 ccacagccag ccggcgagca ttccgatgta gatgcctgtg cagcgagcgc atagcggaag
    25741 ctggtggcca tcgatgtgta gcgaccgttc ggggattcgg tggcaggtgg caaagccgat
    25801 catatcggcc ttagacagtg cgtcgtgcgg cccggcgcat gcgaataggg cgatgacgga
    25861 gagcgccagc ccaacgatca gagactgcca gagggttgca ggaaacgagg cagctcgacc
    25921 ccgccgggcg gggcgcaagc ggcgagtaaa cagacgcatg cgcggagtgt ctccatgcgc
    25981 ccaccccgcg cgtattatac ccgatttgca agcaaagcga ggatcaagat cagcaagaga
    26041 atgacggcga tgaaaagcgc gacggtaagc aagtttgacc agcggtccgg cgacgcggtc
    26101 gcccggcgag gtgccgtatc gtctttgcgc tcgacggtca tcgcgtgaac gggctcggag
    26161 acctgaggcc gggcggaatc cgcgacaaac gccgccgcag gctcaggccc caacgcctcc
    26221 aaaccgcgtt tcgctgccgc gttcgccgga tcgatctcca gcacgcgccg caggcattgt
    26281 cgcttctcgg cgggcgtgtg cacaatgccg ctcatccaca gccacgcctg tacgtcagtc
    26341 gggtcctcgg ccagcgcggc atcgagaagc tgccgggcaa ggctgagatt ccccaccttg
    26401 acggcggcaa tgcctttagg acatctttcc accttcgtat atactattgg caaacgaggc
    26461 tctgcctaac accccatgat gccagctccc ccgtcctccc cgcacacgcg gcccgtgatc
    26521 gtcgtcggcg ccggcatggc tggcctcagc gcggcgctgt cgctggccga gcgcgggatc
    26581 ccgccgctcg tcctcgaagc caatccgcgc ttcgccggcg ggcgcgtggc gggtggccct
    26641 gtgaccgagt tcgagcacgc aggcgaaaca tggcagtttc gtagcgagca tggcattcac
    26701 gggttctggt cggagtatcg aaacctgctc gcattgctcg ggcgcaacaa tatcctgccg
    26761 cgtttcaacc cggccgtcga agaggaatgg attctggcgc ggggcggcgt cgtgcgccgc
    26821 gcggcggccg gcagcgccat acggaacagc ccgtttccgg cgccgttcca ctacgcgatg
    26881 atgtttctgc ggccgcgctt ctggtcgatg ctcgacctgc gggactggct gtcgatcccc
    26941 gtcgtcgtcg gccggctatt cggcgcgatg tcgatcgacc cgctccgcga aggcaagtcg
    27001 ctgcaagggc tgacgcttga ggacttctgc cgaggttggt cgccgtcgct gcgcgccttt
    27061 ttcgcgggac tggcgcgcag cggcctgtcc gcgcaccccg acgaagtgcc gctcgacggg
    27121 ttcatcgctt tcctgcgatt ctacacgctc ctgcggcgcg acgcgtggcg cttcgactac
    27181 ctgcctgccg acgccggcac ggcgctggtc gagccaatgc ttgcgcgctt gcagcacctg
    27241 ggcggcgaag tgatcctcgg cgcgcgcgtg aaccgcctcc aacgcgccgc cggcgggtgg
    27301 cggctgcggt acgatttcga ccggagcacg atctattcag aaagccggca tgtcatcctg
    27361 gccacagacg cgccggccgc gcagcgcatc cttcgcgaca gcccggacac ggccgatctc
    27421 gccgacgcgc tggcattccc gaccggcacg gcgaccgcga tcgttcgcct gtggttcgac
    27481 gccagcccgc gcttcgggcc ggaagcgggc atcttcaccg gcgacttcgt cctcgataac
    27541 ttcttctggc tgcaccgctt ccagaccgac ttcgccatat ggcacgccgc taccggcggc
    27601 agcgctgtcg aggcgcacgt ctacggccca cccgaagtga tcgagcagat ggacgccgtg
    27661 ctgctggcgc aaatgattac cgacgttttc cacgcctggc ccgaactgcg aggccatctc
    27721 attcatcaca tcctgcagcg caacgccgcg acgcacacgc tcttcaagat cggatccggc
    27781 gacgatcgcc tcggcacgca cacgccgtgg accggcatcg ccgcgtgcgg ggactgggtg
    27841 cgccacccca caccggcgct gttcctcgag cgcgcctgcg tcactggcat cgacgcggcg
    27901 aacctcgtgc tcgaagcgct gggcaagccg ccgtggccgc ttgcctctca tgccgggccg
    27961 gaatggatgg ccggtctcct ggagcggttt ttcagaggca cgcgaatggc gctgcacaag
    28021 acgaagcgat aactacccga cgtgcgacgc actctcgccc tcgtttcgac ttgtctccca
    28081 tttcaatcgg ggctaccatt atcgtgcagc accggacacc ggaagcccca agatacgagg
    28141 ccacgccatg gatgatctga tcggccgcac actcggccag tacgaaatcc ttcaacttct
    28201 gggcaaaggc ggcatggcga gcgtctacaa agcctaccag gccagcttgc agcgctacgt
    28261 cgccatcaaa attctcccgc ctcacctcgc ccagaatccc gagtttagca agcgcttcgc
    28321 gcgcgaagcg cgcgccatcg cccagctcga tcatccgaac attgtgcccg tctacgacta
    28381 tggccaggcc gagggtttca ccttcatcgt gatgaaatac gtcgaaggcg gcacgctcaa
    28441 agacatgctc gagcgacgcg gaaggctgcc tctcgaccgc gccgcgctga tcctcggtca
    28501 ggtcgcgtcc gcgctcgact acgcccacgg ccagggcgtg atccaccgcg acatcaagcc
    28561 ggcgaatgtg ctgatgaacc gtcccgattg ggcgctgctg tcggatttcg gcctcgtgca
    28621 catcgccgag gggtcggtga agctgacggg atccggcgtc agcctcggca cgcccgaata
    28681 catgtcgccg gaacaggcgc aggggctgga gatcgacgcg cgatccgaca tttactctct
    28741 cggcgtgatg ctctacgcca tgacgaccgg gcaagcgccg tacaccggca acacacccat
    28801 cgcggtgatt ctcaagcacg tcaacgagcc gctgccctcg ccgcgtgact ccaaccccga
    28861 tttgcccgtt gcggccgaga gcgccattcg caaggcactg gcgaagcatc cggaggaacg
    28921 cccttcaagc gcgggcgagc tgagtcgctc gttcagcacg gcgatcggcg agaaacaccc
    28981 cgtgagccct gccctgagcg tagtcgaagg ggattccgag ccacagcccc gtgtatctgc
    29041 gactccgccg acacctaaga cagaagttgc agcaccgcag gcagcagctc cgctctcgcc
    29101 gcgtcgtaag cagtctctgc tattgatcgc cggggcggcg atcatcgcta tagccgctct
    29161 ggggatcggg gtgctgctcg gcgggcgtag tggagcacca gcaccggcgc cgcctagccc
    29221 gccggcggtg gaatcgactg tgacgcctgc gcctgccgca gcgggcgcct tgcagctccc
    29281 catccgatcg acggtgcaaa tctgggtctt acagaaagcc ggcgaccccc cgcacacagc
    29341 aggcgagggc agggaattcc agtatgcctg gtcaggctcc gggtcgatcg tcacaccgga
    29401 cggcctcatt ctcaccaacg cgcacgtcgt tctccccgac aaggtatatg gcactgtcga
    29461 cggcctcgaa atatggctga ccttccaaga agatgcggcg ccggagccga cgttccgggc
    29521 gcagactgtt gtggcggatc ccgatctcga tctggccgtc gtgaagatca tctccgattt
    29581 ggacggcaat cccgtcgatc ccgacgcgct gaacctgccg gcggtcgagc tgggcgattc
    29641 ggacgagctg tcgatgggtg cagaggtgtt catcctgggc taccccggca tcggcggcga
    29701 gaatatcacg ttgacgaacg gccgtgtggc gggcttcacc aacgcgccgg gagtgaaagg
    29761 acgcgccttc gtcaagacgg atgcgacgat cgcaggcggc aacagcggcg gcctggccgc
    29821 ggatgccggc gggcacctga tcggcgtccc gacgcagctc ggctacggcg gcgaagatca
    29881 gtacgtcgac tgccgcgtgc tggccgacac gaacggcgac ggcgcgatcg acgaacggga
    29941 cgggtgcgtg ccgaccggcg gtttcatcaa cgcgctgcgc ccgatcaacc tggccctgcc
    30001 tctcatccgg caggcgcagg ccggcatcgt gtcgctgccc accgtcgagc cggagcgcga
    30061 gcctatccca acgcctgcga acgccggcga agtgctgttc tccgacgact tcagcagttc
    30121 cgacagcggc tggtcggagg acgaagacga agacatcgcc gcgcgttacg aggacggcca
    30181 catggcgatc gacgtcttca cacagaacta cgtcggctgg acaaaccccg accagtcgtt
    30241 cggcgacgtc gacgtcgaag tcgatacaca gtacctaggc ggcccgctcg acaaccagat
    30301 cggcctcatc gttcgctacc aggatgccga taacttctac gtgttttcga tcagcagcga
    30361 cggcttttac gagttcagca agctcgtcga cggcgagtgg atcacgatgc tgccgtggtc
    30421 gacttcagat ctgatcgatc aggacagcgg ccaaaaccgg atcgccgtgg ttgccgaggg
    30481 cgatcgcttc accttctaca tcaacggcgt gcaggtcgat caagcgtttg acgacgagtt
    30541 cggcgatggc gacatcggcc tgcttgccgg cgcgttcggg gaggggggcg tgtccgtcgg
    30601 tttcgacaac ctgctggctc gcgtgccggg cggctcgcac gaggtgagcg tggacgtgtc
    30661 gcccacggca gacgtgcaaa tgatcctgag cgacagcttc gacgacgaaa gcggcggctg
    30721 gtacgtcggc tcctcgaagg acgtcttgcg gacgattgcg gatggagaat tgacgatcga
    30781 agtcgcagcg gagagttacg ccggctggtc cgatctggag ggcgtcgagc tgcctgacgt
    30841 cgtcgtcgag ctggaggcgc gcaaggtcag cggccccgat ttgaatgcct tcggcctctt
    30901 gtgccgttat caggattcgg agaattacta cgcgctggaa atcggcagcg atagcacgta
    30961 tactatctac cgagtcgcca gcggagagta tgcttcgctg gtggactgga cctcatcgga
    31021 agctatcgcg cctggaacgg acgccaacca actgcgcgtc gaatgcatcg gcgatcacct
    31081 tcgcctgttc gccaacggcg tgctgttggc cgacgtggtc gaccccgctg ggcgaggcgc
    31141 aagcagcgat gcgctcgccg gcggctcggt cgcgctgatc gtagagacgt tcgacgatcc
    31201 cggcgtgcta gtcggcttcg acaacgtagt cgtcggcgtg ccggttgcag gaggcggcgc
    31261 cgaggttctc ttttccgacg acttctcgtc gcccgactcg ggctgggatc gcgcgtccga
    31321 caaggacagc ctgtccgatt acgtcgacgg gcagtacgtg atcggcatca gcgccgagag
    31381 cctctacgta tggtcgaatc ccggccaatc cttcaccgac gtcgtcatcg acgtggatgc
    31441 gacgcaagta gccgggccgg acaacaacga ttacggtgtg ctgtgccgat accaggactt
    31501 tgacaacttc tatcgcatcc tgatcagcgg cgacggttat tacgccttta tccgccaggt
    31561 ggatggagaa tcgacgcagt tgatcgactg gacgcagtcg agcgcgatca accaaggcgc
    31621 cgcacacaat cacctcaccg tcatctgcga cggcgactct ctggcattgt gggtcaacga
    31681 caagttcgtc gcggaagcat cggacggcgc gctgggcgag ggcgacatcg gcctgaccgc
    31741 gggcacgttc gacgaagccg gtgtgcgggt ggcgttcgac aatgttagcg ttacagcgcc
    31801 gtgaggaaga acccatgttt gtgccaagcc tgctcttgaa aaaactttac accacgggaa
    31861 gcctgaagaa caccgagcga ggcgtccagt tttcgatcaa gaatcggctc agcgacgcgg
    31921 agctggtcgc cgtacgaggc atcgccatca acgggacgcc agtgccgctc cagtctctct
    31981 cgctcgacct gggcgacggg cgcatgctat cccctgacca catctcggca gatcgaccgc
    32041 tcgcgtttcc gttacggacg gtcgtcacca ttcaggccgg cgtcgagccg ctgtcgctcg
    32101 gcaagcacga gatcgagatc gccttcgagg ccaaaccgtt cggcaaattg aaattcagcg
    32161 tcgaggatgc gattgcagcg cccgaggaag ggcgcgtgcg catcccgcgc gatccggccg
    32221 acgactattc cgacgagatc gtgcgcgtcc ggcaggcttt cgtcgagcaa tttacggggg
    32281 tgaagcttca ccacgtcagc cattactcat
//
LOCUS       DBNHXB010000306         4470 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1756966, whole genome shotgun sequence.
ACCESSION   DBNHXB010000306 DBNHXB010000000
VERSION     DBNHXB010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1756966"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="AC162G_12380"
     CDS             <1..1196
                     /locus_tag="AC162G_12380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5076662.1"
                     /translation="WYNPAMHDTRLHVAISAHLLASRGAAGYRSAGIHTYIANLLAHL
                     PHLDPGVRYTIYTNAQPPVDARIRPAWLPTAKPLMRILWEQLAQPLALRRDRPDVLHA
                     AAFVAPLASSCPAVVTVYDLSFALFPEHFRGPNQTYLSIFTRLSVRRARRVIAISEHT
                     RRDVHRLYGVPLDHIDVAYPGVDARFRPMPREEVEAFRRKHVLPERFFLYLGTLEPRK
                     NLDRLIDACRGLEIGDWRLVLVGGKGWRVDDLFARVKSHGLEQRVTFAGYAPAEDLPL
                     WYNAATAFVYPSLYEGFGIPPLEAMACGTPVIASSAASLPEVVGDAGILVAPDDVQGL
                     AQSMRRVWRDETLRGDLSRHGNERARRFTWEATARATLESYRRASGNPSSSRSLSSSS
                     SLRGN"
     gene            1389..2816
                     /locus_tag="AC162G_12385"
     CDS             1389..2816
                     /locus_tag="AC162G_12385"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR5076663.1"
                     /translation="MPRRYPRWITMAMVLGDAALVNLAFLSAYILRIELQVFRPVGET
                     YQTPLIDYVPLAAGLTVVQLALFWLGGAYNLRRNTTWLGMMEPIVQGAAVAVLLVIVA
                     TFLDALLLFSRLLIVYNAVLVVIVLGISRALWGLALARLRRRGAGVARALIVGAGEVG
                     RTVLRTIIARPELGFRVMGFVDDHPERSQTDMGAIRALGPITNVPAILDQEPIEEVII
                     TLPWSDHQRILNLVQVCEARGVRPRIVPDLFQLSLTSVDVNDLDGIPLIGTRAPSLSG
                     VSLLLKRIVDLALGVPLTLLSLPLMTLIAVAIKLDSPGPALFRQARLGMNGRPFQVYK
                     FRSMYSGAEAEVPQLLERNEASGPLFKIRDDPRRTRVGRFLRRTSFDELPQLINVLRG
                     EMSLVGPRPPLPSEVDQYKPWHKQRLLARPGMTGLWQVSGRSSLSFDEMVLLDIYYIE
                     NWSPLLDVKIMLRTVPKVLSGDGAY"
     gene            2844..3725
                     /locus_tag="AC162G_12390"
     CDS             2844..3725
                     /locus_tag="AC162G_12390"
                     /inference="COORDINATES: protein motif:HMM:NF015587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR5076664.1"
                     /translation="MRVRAVFDLIRETFEAWTNDRAERLGAAFAYYALFSMTPMLVVA
                     IVIAGSVFGQAAALDQIVETVENLFGPETAQTIESMIDSMHDAKPTLIGTLISLGLLL
                     YFASGLFANLKDALNLVWGVRPKPGHGVRHFIQTHILAILMVLCMGFLLMLALALNTL
                     TAFLSSFFGDRLPGGVLAWQVLNFAASFALILLLVVVIYRVLPDAKIAWRDIAVGAAV
                     TSLLVTIGTLAIGIYLALGDWISSTFGAAGPIVAVLIWAYYFAQVFFFGVEFTQVYAR
                     QRGSGIRPRWNPPQPND"
     gene            3748..>4470
                     /locus_tag="AC162G_12395"
     CDS             3748..>4470
                     /locus_tag="AC162G_12395"
                     /inference="COORDINATES: protein motif:HMM:NF024956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR5076665.1"
                     /translation="MPLVSIGIGWLIGIWAAFSFALPWQLFLVALVIPFAGLILWRGD
                     PRARLVWISLLAMLLGGLRAASARPSLDEHDVATYNDGSLVTLAGVVDDYPDRRDTYV
                     NLRLRAETLAWPDADPFAVEGLVLVRADPLGDYRYGDRLEVTGHLQTPPEFATFNYRD
                     YLAGKGIHSIVDQPRVGRLVRDQGSPILAALYDLKDRARETIAAILPEPQAALLTGIL
                     LGDESGIPQDVKDDFRATGTSHI"
BASE COUNT          711 a         1414 c         1451 g          894 t
ORIGIN      
        1 tttggtataa tccagccatg cacgatacgc ggctccacgt cgcgatcagc gcgcatttgc
       61 tcgcttcgcg cggggcggcg ggatatcgca gcgccggcat ccacacgtac atcgccaact
      121 tgctcgcaca tctgccgcac ctcgatccgg gcgtgcgtta cacgatttac acgaacgcgc
      181 agccgcccgt cgacgcgcgg attcgcccgg cgtggctgcc gacggcgaag ccgctgatgc
      241 gtatcctgtg ggagcagctc gcccaaccgt tggcgctgcg ccgcgaccgg cccgatgtgc
      301 tgcacgcggc ggcgttcgtc gcgccgctcg cgtcgagttg tccagccgtc gtcacggtgt
      361 atgacttgag ttttgctcta tttccggagc actttcgcgg tccgaaccag acctatctga
      421 gcatcttcac gcgcctctcg gtgaggcgtg cgcggcgcgt catcgccatc tccgagcata
      481 cacggcgcga cgtgcatcgc ctgtacggcg tgccgctcga tcacatcgac gtcgcctatc
      541 cgggcgtgga cgcccgattc cggcctatgc cgcgcgaaga ggtcgaggcg tttcggcgca
      601 aacatgttct tcctgaaagg ttcttcctgt acctcggcac gctagagccg cgcaagaatt
      661 tggaccggtt gattgacgcc tgtcggggat tggagattgg agattggaga ttagtgctgg
      721 tcggcggtaa gggttggagg gtcgacgatc tgtttgccag ggtgaaatcg cacggtctgg
      781 agcagcgggt gacgttcgcc ggttacgcgc ctgcggaaga cctgcccttg tggtacaacg
      841 cggcgacggc gttcgtgtat ccgtcgctgt acgagggctt cggcataccg ccgctggagg
      901 cgatggcatg cggcacgccg gtcattgcat cgagcgcagc gtctctgccg gaagtggtcg
      961 gcgacgccgg catcctcgtc gcaccggatg atgtgcaggg gctggcccag tcgatgcgcc
     1021 gcgtgtggcg cgacgaaacc ctgcgcggcg atctgtcgcg gcacgggaac gagcgagcgc
     1081 ggcggttcac gtgggaagcg acggcgcgcg ccaccctcga gtcgtaccgg cgagcgtcgg
     1141 ggaacccgag ttcctcccgt tccttgagtt cctcgagttc cttacgaggg aactgaagga
     1201 acttagggaa gaaacttgat tatggactgg agtttggcag ttcgctggag tgcagccctt
     1261 cggcaggctc cccactcccc ctcgctttgc tcgggggcgg ggacttcgca gggcaggctc
     1321 ttcaggctgc catatgtggc ggtcaagcag gctaaagcct gcactccaac cgctgaagga
     1381 tggaagtgat gccgcgccgg tatccacgct ggatcacgat ggcgatggtt ctgggcgatg
     1441 cggcgctggt gaacctggcg ttcctgtccg cctacatcct ccgcatcgag ctacaggtgt
     1501 ttcgtcctgt cggcgaaacg taccagacgc cgctgatcga ttatgtcccg ctggcagcgg
     1561 gcctgaccgt cgtgcaactg gcgctgttct ggctgggcgg cgcgtacaac ctgcgccgca
     1621 acacaacctg gctcggcatg atggagccga tcgtgcaggg cgcggccgtc gcggtgctgc
     1681 tcgtgatcgt cgccaccttt ctcgacgcgc tcctgttgtt ctcgcgcctc ctgatcgtgt
     1741 acaatgccgt gcttgtcgtg atcgtcctgg gcatcagtcg agccttgtgg gggctggcgc
     1801 tggcgcgcct gcggcggagg ggcgcgggcg tggcgcgcgc gctgatcgtc ggcgccggcg
     1861 aggttggccg caccgtgctg cgcacgatca tcgcacgccc ggagcttggg tttcgggtga
     1921 tgggttttgt ggacgatcac ccggagcgca gtcagaccga catgggcgcg atccgcgcgc
     1981 tggggcctat cacgaacgta ccggccatcc tggatcagga gccgatcgaa gaggtgatca
     2041 tcacactgcc ctggtccgat caccagcgca tcctgaacct ggtgcaggtg tgcgaggcgc
     2101 ggggcgttcg cccccgcatc gtgcccgacc tgttccagct cagcctgacg agcgtcgacg
     2161 tgaacgatct ggatggtatt ccgctgatcg gcacacgtgc gccgtcgctc agcggggtaa
     2221 gcttgttgct caagcgtatc gtcgatctcg cgctgggcgt ccccctgacg ctgctgtcat
     2281 tgccgctgat gaccctcatc gccgtcgcca tcaagctcga ttcgcccggg ccggcgctat
     2341 tcaggcaggc gcgcctcggt atgaacggcc ggccgttcca ggtctacaag ttccgctcga
     2401 tgtattccgg cgcggaagcg gaggtcccgc aattgctcga gcgcaacgaa gcgagcggcc
     2461 cgctgtttaa gatcagagac gatccgcgcc gcacgcgcgt cgggcgcttc ctgcgccgta
     2521 ccagcttcga cgagctgccg caactcatca acgtgctgcg cggcgagatg agcctggtcg
     2581 ggccgcgccc accgctgccc tccgaggtcg atcagtacaa gccgtggcac aagcagcggc
     2641 tcctggcgcg gccggggatg accggcttgt ggcaggtatc cggccgcagc agcctgagct
     2701 tcgacgagat ggtgctgctg gacatctact acatcgagaa ttggtcgccg ctgctggacg
     2761 tgaagatcat gctgcgcacc gtgccgaagg tgctgtcggg ggatggggcg tattagccga
     2821 tggcggatgg ctgataatag agcatgcgcg tcagagcggt cttcgacctc atacgagaaa
     2881 cgttcgaggc gtggacaaac gatcgggccg agcggctggg cgcggcgttc gcctactatg
     2941 ccctgttttc gatgacgccc atgctcgtcg tcgccatcgt catcgccggg tcggtattcg
     3001 ggcaggcggc ggctttggat cagatcgtcg agacggtcga gaacctattc gggccggaga
     3061 ccgcacagac gattgagagc atgatcgaca gcatgcacga cgccaaaccc acactgatcg
     3121 gaacgctgat cagcctgggc ttactgctct acttcgcgtc gggcctgttc gccaatctca
     3181 aggacgcgct caacctggtg tggggcgtga ggccgaaacc cggccacggc gtgcgtcact
     3241 tcattcagac gcacatcctg gccatcctga tggtgctatg catgggattt ctgctcatgc
     3301 tggcgctggc attgaatacg ctgaccgcct ttctgagcag ctttttcggc gatcggctgc
     3361 ccggcggagt gctcgcctgg caagtgctga acttcgctgc gtctttcgcg ctgatactgc
     3421 tgctcgtcgt cgtgatctat cgcgtgttgc ccgacgcgaa gatcgcctgg cgcgacattg
     3481 ccgtcggcgc ggccgtgacg tcgctgctcg tgacgatcgg cacgctggcg atcggcatct
     3541 atctcgcgct cggcgactgg atatcgtcga cgttcggcgc ggccgggccg atcgtcgccg
     3601 ttttgatctg ggcctactat ttcgcgcagg tattcttctt tggcgtggag ttcacgcaag
     3661 tgtacgcccg gcagcgtggc tcgggcatcc ggcccagatg gaacccgccg caaccaaacg
     3721 actaaccacg gattacacaa aattcatatg cctctcgttt cgatcggcat cggctggttg
     3781 attggcatct gggcagcatt cagcttcgca ctcccgtggc agctctttct cgtcgcgctt
     3841 gtcatcccct ttgccggtct gatcctgtgg cgcggcgatc cacgtgctcg actcgtatgg
     3901 atatcgctgc tcgccatgct gctcggcggg ctgcgcgccg catcggcccg gccttccctc
     3961 gacgagcatg atgtcgccac atacaacgac ggcagcctcg tcacgctcgc cggcgtcgtc
     4021 gacgactatc ccgaccggcg tgacacgtac gtcaacctgc gcctgcgcgc ggaaacgctg
     4081 gcatggcctg acgccgatcc gtttgccgta gagggtctgg ttctcgtgcg tgccgatccg
     4141 ctcggcgact accgttacgg cgatcgcctc gaagtgaccg gccaccttca aacgcctcct
     4201 gagttcgcca cgttcaacta ccgggattat ctggcgggca aaggcattca ctcgatcgtc
     4261 gaccagccgc gcgtcgggcg cctcgtgcgc gaccaaggca gcccaatcct cgcagcgctt
     4321 tatgacctga aagatcgcgc gcgagagacg attgccgcga tactgccgga gccacaggcg
     4381 gcgctgctga ccggcatcct gctcggcgat gagtcgggca ttccgcaaga cgtgaaggac
     4441 gacttccgcg cgacgggcac gagtcacatc
//
LOCUS       DBNHXB010000307         2520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4106898, whole genome shotgun sequence.
ACCESSION   DBNHXB010000307 DBNHXB010000000
VERSION     DBNHXB010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4106898"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="AC162G_12400"
     CDS             complement(<1..599)
                     /locus_tag="AC162G_12400"
                     /inference="COORDINATES: protein motif:HMM:NF021379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HIR5076666.1"
                     /translation="MLAPMCYNRVRLQDEPVPMVSKPFGQALATPAKTARPSPESVVL
                     IALITICAILFVYLSVARHYTFSTAAYDLAMYDQAVWNTSQGRWFEINLLEDTMPDLT
                     NKLGDHVEPILLPIALLYRLRANADVLLILQALALAALIWPLYHVVWHLTGRLLLAGV
                     AVALYLAHPAMWNALLFDFHPVTLAAAFLMFALWMLIQK"
     gene            complement(634..1443)
                     /locus_tag="AC162G_12405"
     CDS             complement(634..1443)
                     /locus_tag="AC162G_12405"
                     /inference="COORDINATES: protein motif:HMM:NF016163.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HIR5076667.1"
                     /translation="MSYPRRIHGIDFSGAADAGRRIWIASGAIESGRLHIDACRRADT
                     LPGSSHERDVCLAALRSFIAGEKACAVGLDFPFGLPASLMDASSWDDFIHSFPNRYPG
                     PQEFRAHCMHTAGGAERKRATDSESRAPFSPYNLRLYRQTYHGIRDVLYPLVRDRQAC
                     VLPMQRAQSGKPWLLEICPASMLKQLNLYRPYKGSTRAHHLARRYILSTIGARLDVSI
                     SRATQPMLLSDSNGDALDSVIAAYVASRTVRDPERLTPQDRLHTLEGYIYI"
     gene            complement(1412..2449)
                     /gene="cofD"
                     /locus_tag="AC162G_12410"
     CDS             complement(1412..2449)
                     /gene="cofD"
                     /locus_tag="AC162G_12410"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011254950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HIR5076668.1"
                     /translation="MLVVALAGGIGGAKFADGLARVPSVPLVPVHLQGGDQTIELTVI
                     VNIGDDFEHLGLRICPDLDTVVYTLAGIANPETGWGIAGETWHAMDMLDRFGGETWFR
                     LGDRDLGMHLFRTAALKRGKRLTEIVAEACTRLGVTARVLPATDSPVPTIVETDQGAL
                     DFQEYFVHRRCEPVVKRLRFDGAESARPTDEALAAIEAADLVVVCPSNPFLSIDPILV
                     IGDLRERLRRKPVVGVSPIIGGQAVKGPAAKMLRELGYESSALSVAKHYADFLDMFVI
                     DEVDGDQRLEIQRLGVRVLTTDTLMKSVDDRARLAREVIAFAILHDRPTTTGDATAGA
                     PRELPASNTRH"
BASE COUNT          516 a          799 c          752 g          453 t
ORIGIN      
        1 ttcttttgaa tcagcatcca cagcgcgaac atcagaaaag ccgccgccag cgtgaccgga
       61 tggaaatcga agagcagtgc gttccacatt gccggatgcg ccaggtacag cgccacggcc
      121 acgcccgcca gcaggaggcg gcccgtcaag tgccaaacga cgtggtacaa gggccagatc
      181 agcgcggcca gcgccagcgc ctggaggatg agcagcacgt cggcgtttgc gcgaagccgg
      241 tacagcaagg cgataggcag caagatcggc tcgacgtggt cgcccagctt attggtgaga
      301 tcgggcatcg tgtcttcgag caggttgatc tcgaaccagc gaccctggct ggtgttccac
      361 accgcctggt cgtacatcgc cagatcgtag gccgcggtgc tgaacgtgta atgccgcgcc
      421 accgagagat agacgaacag aatcgcgcag atggtgatca gcgcgatcag gaccacagat
      481 tccggcgaag ggcgggcggt cttcgcaggt gtggcgaggg cctgcccgaa cggcttactg
      541 accatgggaa ccggctcgtc ctggagccgg acgcgattgt aacacatcgg cgccagcaag
      601 cggcattctc catcgtaggc agctcgtcgg cgttcagatg taaatgtagc cctccagagt
      661 gtgcaggcgg tcttgagggg tcagacgctc cggatcgcgg accgtgcgag aagccacata
      721 ggcggcgatg acgctgtcga gcgcgtcacc gttggaatcg ctcagcagca tcggctgtgt
      781 agcccgtgag atggacacat caagcctagc cccaatcgtc gataggatgt acctgcgcgc
      841 caggtgatgg gcacgcgtgc tgcccttata cggtcgatag agattcaatt gcttcagcat
      901 cgacgccggg cagatttcga gtagccacgg cttgccgctc tgcgcacgct gcatgggcag
      961 cacgcacgcc tgcctgtctc gcacaagcgg gtaaagaacg tcgcggatgc cgtggtacgt
     1021 ctgccggtat aagcggagat tgtaaggtga gaatggcgcc cgactctcgc tatcagtagc
     1081 gcgctttcgc tcggcgccgc cggccgtatg catgcagtgc gcccggaatt cctgcgggcc
     1141 ggggtatcga ttcgggaacg agtggatgaa atcgtcccag cttgacgcat ccatcaggct
     1201 cgcaggcagg ccgaagggaa agtcaagccc cactgcgcat gctttctcac ccgcgatgaa
     1261 gctgcgcagc gcggccaggc acacgtcgcg ctcgtggctc gaacccggca gggtgtctgc
     1321 ccggcggcag gcgtcgatgt ggagacgacc tgattcgatc gcgccgctcg cgatccagat
     1381 gcgccggccg gcgtcggcag cgccgctgaa gtcaatgccg tgtattcgac gcgggtaact
     1441 cacgaggcgc tccggcagtg gcgtcacccg tcgtagtcgg tcggtcatgg aggattgcga
     1501 acgcaatgac ctctctcgcc agcctcgccc gatcatccac cgacttcatc agcgtgtctg
     1561 tcgtgaggac gcgaaccccc aatctctgaa tctccaatct ctgatctcca tctacctcat
     1621 caataacgaa catatccagg aaatccgcgt aatgcttcgc cacgctcaat gccgaagact
     1681 catagcccag ctcgcgcagc atcttcgccg ccgggccttt gactgcctgc ccgccgatga
     1741 tcggcgacac gccgacgacc ggcttgcgcc tcagccgctc gcgcaaatcc ccaattacta
     1801 atatcggatc gatgcttaaa aatggattgg aaggacacac cacgaccaga tcggccgcct
     1861 cgattgccgc aagcgcctcg tccgtgggcc gcgccgattc ggccccatcg aagcgcagcc
     1921 gtttgacgac cggctcacaa cgccgatgga cgaagtactc ttgaaaatcg agcgcgccct
     1981 gatcggtctc gacgatggtc gggacgggcg aatccgtcgc aggcagcaca cgcgccgtta
     2041 cgcccagcct ggtgcacgcc tcggcgacga tctcggtcag acgtttgccc cgtttcaaag
     2101 ccgccgtccg aaacagatgc atgcccagat cgcgatcccc cagccggaac cacgtctcgc
     2161 ccccgaagcg atcgagcatg tccatcgcat gccacgtctc gccggcgatg ccccagcccg
     2221 tctcgggatt ggcgatgccc gccagcgtgt acaccacagt gtcgaggtcg ggacatatcc
     2281 gcaggccgag atgctcgaag tcgtcaccga tgttgacgat cacggtgagt tcgatagtct
     2341 gatccccgcc ctggagatgg acaggaacta aaggaactga aggaactcgc gctagcccat
     2401 ctgcaaactt cgcccctccg ataccaccag ctaacgcgac aactagcatt gattatcgtt
     2461 tggtctcact ttctgccttg tggcgtttga acacatataa tccctcgtct ggacatcagt
//
LOCUS       DBNHXB010000308         8031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1256809, whole genome shotgun sequence.
ACCESSION   DBNHXB010000308 DBNHXB010000000
VERSION     DBNHXB010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8031
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1256809"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="AC162G_12415"
     CDS             <1..648
                     /locus_tag="AC162G_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076669.1"
                     /translation="PHASLVFPRGQGQARSLPWVITQAVAPVDAAFAGLFAAHAEKTA
                     AIDLRPDDFNPRFEVYRFDAERAVREALESATRLPGSASFDGALRLLGYDIRTPQARP
                     GDTVEVMIYWQFTSPVPFGKQAVLFTHVLTGDPGRPVLAQQDSLDVPLESLASGDVFA
                     QVHRFVIPPDAAPGAYSLEVGVYTREDGARLPVRDESGALAGDHVIIGSIEIVVP"
     gene            698..2686
                     /locus_tag="AC162G_12420"
     CDS             698..2686
                     /locus_tag="AC162G_12420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR5076670.1"
                     /translation="MTKQSLTPNLQSPISNYWALVAILLIAAAFRFAAIGDAPPGWRD
                     DELIEFDMDRRIVQGWRPLFIAEAEGHEPFYHYLHAATIALFGENRVGYKWLPFAFGV
                     ASVALTFALARRMFDARVAALAAALMAISFWPVMYARLGLRHIGVVPLMLGAFCLLYP
                     RPLAASKLQTSRSKIPNSLAAGLLLGAALITYFAGRALPLILAGFLAYLLVFHRAALR
                     RVWLHDVAAIVLAVVIAAPMFVEIGRTPGAEKRTEVVGGPFIAALRGDLRPALETTLG
                     TLGMFTVVGDPESLYNVPGRPVFDGLAGLCFYVGVLWCLVRIKQMGSGFALVWLLVGI
                     APAFVSVPAASFSHTIAAQPVVYMLAAYGAVEAVAWIANRAVGQQARAWSFGAVSLLL
                     VTVSAALTLRDYFGAWAGDPFVRFQYHAPTRDIARWLDQEAAVTDVAIGTHPNYLSLD
                     PLALELDLVREGVMARWFSPETTLLIPLNGVLIFSSTQAPGSDVHPWIESLRNESILR
                     KLGSAPGGPEAYAVNSDIWQPIDPHGVLATFGDSIGLLGGGATETSAAPGDRVSVLTI
                     WLLVKPPPASVLKSFVHVLDAQNQVVAGDDRFDVNVSSLRPGDYIGQFSSLTLPAGLA
                     SGRYQIEVGLYYPETGVRLTAADGGDRALINPIEVTAP"
     gene            2683..4695
                     /locus_tag="AC162G_12425"
     CDS             2683..4695
                     /locus_tag="AC162G_12425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR5076671.1"
                     /translation="MITHHASRTTSFTPRRELALVVALLLIAFFFRTHELAGLGLGLE
                     HDEVAEWLIADGIRKGQHALFFEEAYGQEPLFLYLMTGSIALLGDNVIGVRFTSAFVA
                     MLALAAAYRMLRRMFGPTVALVALAGLSIALWPVFWGRVGLRAMTLPLALCLGFDFLW
                     RGLTAECGMQNAECRKWLVWAGAFVGLSAYTYLSARAVPILLAAFALYLALFARDRMR
                     DRWKHLALFLAIAAVVALPLALAVLANPDLQFRVSEVSAPLDKALQGDWSDVSANIPR
                     ALGMFALRGDGTVRDNWPDRPVFPEPIGGLLFSLGFLVALTRMRQPRYALALIWIGAM
                     LIPTIVTTGAPNFTRALGTLPMVFALPGIAVDWLAFWAPRWIDVTRFHVVRRIAAPLF
                     ALLLLAVFAVNAASTYDDYFNKWPQHPETQFVFQADFAAIAKDIDVSGVMDVAVGGLS
                     NDTLDDPSLYLLRQRKDVRVRWFDPGSPLSSGGAIVAWYGKPLTTYVPSIVPVEPLLA
                     DDLRPNEHYELIETDRFRRYHINSNAVPGIAPDAVTFEDTALLQPVGVEEIPAVRAGS
                     GLQLQTVWIARAPIAYPRRIFVHVVDPATGRIIAQHDGLDAPSRFWREGDYVTQLHTL
                     EIPASTVAGRYELWIGLYDPASNRRVPVQSDPPGDHAVLGPIKVTK"
     gene            complement(4770..5747)
                     /locus_tag="AC162G_12430"
     CDS             complement(4770..5747)
                     /locus_tag="AC162G_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076672.1"
                     /translation="MGFTRLRRITPAAASLALAVTMLSLLFLISSPVTHAMEDVPALP
                     EASTVHEPGLESSLQQTATNTYTAYLPLLLKPAFLYLPLLFYNRAFAYDYGFEDPGCD
                     GWVIHYFDPYEPDPPGPWTSFCGKEKFDRYYGDNGVYSFKTSAAWNTWIYTAPIVLAD
                     TRNFTIELDGKSAQDFMWASAWGVYFNANADRTKFYSIQIHQTGVPDLDTPPQYSIRR
                     WINFRGTGTDQNDILVFRRCGPCAQPDFQWNTVIVRRTGDWVTFYAGDKSNPLSLQKP
                     LRVLYLPDYTSAEYNGMGVLQGNFEFQNWNGDDPAFQVDNFYADPVYRR"
     gene            5923..>8031
                     /locus_tag="AC162G_12435"
     CDS             5923..>8031
                     /locus_tag="AC162G_12435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR5076673.1"
                     /translation="MSSRRHPPAQAAGIVLLLVLAYLQFSTLAVTSPTLDEPNHLVSG
                     YAFLTRGDTRIKLNGPILPNALGALPLLLQPGLVLSPPDDPGWDTNQYNDIADRFLWS
                     NTASPFHIIYLARFPFMAAGWLLGALIFRWARERSGPRAGLFALAFYVFCPNLLAHSR
                     FVMTDFVPAALTCLTLYTFDRSMHSDRPGWAIAAGTALGLALASKFSLVMLIPAMAIL
                     LFLRAGWQSVRRPLGQLALTVLVAAVAIWGVYSFQVAPVSAGGLPVPAPGFWGEVESA
                     RYVLGQPWPNYLFGQISDSGWWYYFPIAFLVKTPLPMLILFAAALVRTVRARSWRRDA
                     VFLLPSFLLVFGLLFSTNNLGYRYLLPLLPLIFVYASDLVPKSSFALSPRHLVTLSLL
                     AWHIIGTLRIYPFYLTFFNEIAGGPDRGRYVLSDSNIDWGQDMAGLKQYVEENGIDRI
                     KLSYFGIAHPTAYGLKAEPLLPVRTAMHDQGAWWLHTFYPHDPPPGVYAISVANLMGG
                     IWIDRETYAYFRDRKPDTTIGNAIYIFTISPRGEPVDLSLAGLQIDQIDPDTYTRFGT
                     NDVRPRWFDETTSLIAAPGESWVAISDLQSLPPEFAVLLDDVQPIARPRTVDDGQPYR
                     LYRLDLARRMRGAAAQAEQVVQEGVLPATFGRTAELIGYSLAEDSERSPGITLVTYWR
                     AGDDVVTPLQLFVHAVDASDQ"
BASE COUNT         1286 a         2592 c         2483 g         1670 t
ORIGIN      
        1 ccgcacgcca gcctggtgtt tccgcggggg caggggcaag cccggtccct accctgggtg
       61 atcacccagg cggtcgcgcc ggtcgatgcc gcctttgccg ggctgttcgc cgcgcatgcg
      121 gagaagaccg ctgccatcga cctgcgcccc gacgatttca atccgcgctt cgaggtgtac
      181 cgcttcgacg ccgagcgcgc ggtgcgcgag gcgttggagt cggccactcg cctacccggc
      241 agtgcctcgt tcgacggcgc gctgcgcctc ctgggttacg acatccgtac gccgcaggca
      301 aggccgggtg acacggttga agtgatgatc tactggcaat tcacatcgcc ggtcccgttc
      361 gggaagcagg cagtcttgtt cacccacgtc ctgaccggcg atccgggccg gccggtgctg
      421 gctcagcagg attcgctcga cgtgcctctg gaaagtctgg cttccggcga cgtgttcgct
      481 caggtgcatc gcttcgtcat cccgccggat gccgcgcctg gcgcatattc gctcgaggta
      541 ggcgtttata cgcgcgaaga cggcgcccgc ctgccggtgc gcgacgagag cggagcgctc
      601 gcgggcgacc acgtcatcat cggctcgatt gagatcgtag taccgtgagg agtagcaaga
      661 agcaagagac aagaggcagg atggcctcgc tgactcgatg acaaagcaat ctctaacccc
      721 caatctccaa tctccaatct ccaattactg ggcgctggtc gccattctcc tgatcgccgc
      781 cgcgtttcgc ttcgccgcga tcggcgacgc gccgcccggc tggcgcgacg acgagctgat
      841 cgagttcgac atggatcggc gcatcgtcca gggttggcgg ccgctgttca tcgccgaggc
      901 ggaaggccac gagccgtttt atcactacct gcacgcggcg acgatcgcgc tgttcggcga
      961 gaaccgcgtc ggctacaagt ggctgccgtt cgccttcggc gtggcgagcg tcgcgctgac
     1021 gttcgcgctg gcgcgccgca tgttcgacgc gcgcgtcgcg gcgctggcgg cggcgctgat
     1081 ggcgatttcg ttctggccgg ttatgtacgc gcgcctgggc ctgcggcaca tcggcgtcgt
     1141 gccgttgatg ctgggcgcgt tttgcttact ctacccgcgt cctttagccg cttccaaact
     1201 ccaaacttca agatccaaaa tcccaaatag cctggcggcc ggcctgctcc tcggagccgc
     1261 gctgataacg tacttcgcgg ggcgggcgct gcccctgatc ctggccggct tcctggccta
     1321 tctgctcgtc ttccaccgcg cggcgctgcg gcgggtctgg ctgcatgacg tggcagccat
     1381 tgtcctcgcc gtcgtcatcg ccgcgccgat gttcgtcgag atcgggcgca cccccggcgc
     1441 ggagaagcgc accgaagtcg tcggcgggcc gttcatcgca gccctgcgcg gtgatctgcg
     1501 gcccgcgttg gagacgacgc taggcacgct cggcatgttc accgtcgtgg gcgaccccga
     1561 atcgctctac aacgtccccg gtcgccccgt gttcgatggg ctggcgggcc tgtgcttcta
     1621 cgtgggcgtg ctgtggtgcc tggtgcggat aaagcagatg gggtccggct tcgcgctcgt
     1681 ctggcttctc gtcggcatcg cgcccgcgtt cgtcagcgtc ccggcggcga gcttcagcca
     1741 cacaatcgcc gcgcagccgg tcgtgtacat gctcgccgcg tacggcgcag tggaagctgt
     1801 cgcgtggatc gcgaatcgag ctgtcggtca acaagctcgc gcgtggtcat tcggcgcagt
     1861 gagcctgctg ctcgtgaccg tatctgctgc gctcacgctg cgcgactact tcggcgcgtg
     1921 ggcaggcgat cccttcgtgc ggtttcagta tcacgccccg acgcgcgaca tcgcccgctg
     1981 gctcgatcag gaagcagcag tcactgacgt cgccatcggc acacatccga actatctgtc
     2041 gctcgatccg ctggcgctgg agcttgacct ggtgcgcgaa ggcgtgatgg cgcggtggtt
     2101 tagccctgaa acgacgctgc tcatccctct gaatggggtg ctgattttct cctcgacgca
     2161 ggcacccggc agcgatgttc acccctggat cgaatcgctg cgcaatgagt cgatcttgcg
     2221 gaaactggga tctgcgcccg gcgggccgga ggcatatgcc gtcaattccg atatctggca
     2281 gcctattgat ccgcacggcg tgcttgcgac ctttggcgat tcaatcggat tgctcggcgg
     2341 aggtgcgacg gagactagcg cggctccggg cgatcgggtc tctgtgctga cgatctggct
     2401 gctcgtcaaa ccgccccccg catcagtgct gaagtcgttt gttcatgtct tggacgcaca
     2461 gaatcaagtc gtggccggtg acgatcgatt cgatgtcaac gtgtcgagcc tgcggcccgg
     2521 cgattacatc ggtcaattca gcagcttgac gctgccggcc gggctggcgt cgggccgcta
     2581 tcaaattgaa gtgggtctat actacccgga gacaggtgtt cgtttaacgg cggcggacgg
     2641 cggcgatcgg gctctgatca accctatcga agtgacggcg ccatgatcac gcatcacgcg
     2701 tcacgcacca cttctttcac tcctcgccgc gagctggcgc tcgtcgtcgc gctgctcctc
     2761 atcgctttct tctttcgcac gcacgagctg gcgggactgg gcctcggcct ggagcacgac
     2821 gaagtcgccg aatggctgat cgccgacggc atccgcaaag gacagcacgc gctctttttc
     2881 gaagaagcct acggccagga accgctgttc ctgtatctga tgaccggctc gatcgcgctg
     2941 ctcggcgaca acgtgatcgg cgtgcgcttc acctctgcct tcgtggcgat gctggcactc
     3001 gccgcagctt accggatgct gcgccggatg ttcggcccga cggttgcgct cgtcgcgctg
     3061 gccggcctgt cgatcgcgct gtggccggtg ttctgggggc gcgtcggact gcgggcgatg
     3121 acgctgccgc tggcgctgtg cctgggattc gatttcttgt ggcgcggact cacggcggaa
     3181 tgtggaatgc agaatgcaga atgtagaaag tggctcgtgt gggccggcgc gttcgttgga
     3241 ttgtcggcgt acacctatct ttcggcgcgc gccgtgccga ttctgctggc tgctttcgcg
     3301 ctctacctcg cgctcttcgc acgcgaccgg atgcgagatc gatggaaaca cctggcgctt
     3361 ttcctggcga tcgccgccgt cgtcgcgtta cctctggcgc tcgcggtgct ggcaaatccc
     3421 gacctgcaat tccgtgtctc cgaagtcagc gccccgctcg acaaggcgct gcaaggcgac
     3481 tggagcgatg tatctgcgaa cattccccgc gcgctgggta tgttcgcgct gcgcggcgac
     3541 ggcacggtgc gcgacaactg gcccgaccgc cccgtgttcc ctgagccgat cggtgggctg
     3601 ctgttcagcc tgggcttcct cgtcgcgctc acgcgcatgc gccagccgcg ctacgcgctc
     3661 gcgctgatct ggatcggcgc gatgctgatc ccgacgatcg tcacgaccgg cgcgccgaat
     3721 ttcacgcggg cgctcggcac gctgccgatg gtcttcgcgc tgcccggcat cgcggtagac
     3781 tggctggcct tctgggcgcc gcgctggatc gatgtgacgc gcttccacgt cgttcggcgc
     3841 atcgccgcgc cgctcttcgc tctgctcttg ctcgccgtct tcgccgtcaa cgccgcctcg
     3901 acctacgacg actatttcaa taagtggccg cagcatcctg agacgcagtt cgtcttccag
     3961 gccgacttcg ccgcgattgc gaaggatatc gacgtgagcg gcgtgatgga tgtcgccgtc
     4021 ggcggcctgt cgaacgacac gctggacgat cccagcctgt acttgctgcg ccagcgcaag
     4081 gacgtgcgcg tgcgctggtt cgatccgggc agcccgctga gcagcggtgg cgcaattgtc
     4141 gcttggtatg gcaaaccgct cacgacctac gttccctcga ttgtgccggt tgagcctctg
     4201 ctggcagacg acctgcgtcc caacgagcat tacgaactga tcgagaccga tcgctttcgg
     4261 cgttatcaca tcaactccaa cgcagtcccg ggcattgcgc cagacgccgt cacttttgaa
     4321 gacacggcgc tgcttcaacc cgtgggcgtg gaagaaatac cagccgttcg tgcagggagt
     4381 ggcctgcaac ttcaaaccgt gtggatcgcc cgagcgccga ttgcttatcc caggcgcatc
     4441 tttgttcacg tcgtcgatcc ggcgacgggt cggatcatcg cgcagcatga tggcctggat
     4501 gcgccgagca ggttctggcg cgaaggtgat tatgtcaccc agctccacac gctcgagatt
     4561 cccgccagca ccgtggctgg ccgttacgaa ctctggatcg gcctgtacga tcccgcgtcg
     4621 aatcggcgag tgccggtaca atccgatccg ccaggcgatc acgccgtcct cggcccgatc
     4681 aaagtgacga aatgaaaaac gcatggccgc tcggttgagc agccatgctg atcctgtccg
     4741 cttattcgat tggcgattgc cggcctgggc tagcgacggt aaaccgggtc ggcgtagaag
     4801 ttgtcaacct ggaaagccgg atcgtcgccg ttccagttct ggaactcaaa attgccctgg
     4861 agcacgccca tgccattgta ttcggcgctc gtgtaatccg gcaggtataa aactctcagt
     4921 ggcttctgga gcgacaatgg attggacttg tctccggcgt agaaggtgac ccagtcacct
     4981 gtgcgccgga cgatgaccgt gttccattgg aaatctggct gagcgcaagg cccgcagcgc
     5041 ctgaacacga ggatgtcatt ctggtcggtg ccggtgccac ggaagttgat ccagcgccga
     5101 atggagtact gggggggcgt gtccagatcc gggacgccgg tttggtggat ctggatcgag
     5161 tagaacttcg tccgatcggc gttcgcgttg aagtagactc cccacgccga cgcccacatg
     5221 aagtcctgcg ccgacttgcc gtcgagctcg atcgtgaagt tgcgggtatc cgccagcacg
     5281 atgggcgccg tgtagatcca cgtgttccac gcggcggaag tcttgaagct gtacacgcca
     5341 ttgtcaccgt agtaacggtc gaacttttct ttgccgcaga aggaagtcca gggaccgggc
     5401 gggtcaggct cgtatggatc gaaataatga atgacccagc cgtcacagcc agggtcctca
     5461 aacccgtaat catatgcaaa agcacggttg tagaacagca gcggaaggta caggaaagcg
     5521 ggcttgagta acagcggaag ataagcggta tacgtgttcg tcgcggtctg ttggagggag
     5581 gattcaagtc caggctcgtg aacggtagat gcttctggaa gtgcgggaac atcttccatc
     5641 gcatgggtca caggtgagct gattaagaac aaaagcgata acatcgtaac cgcgagcgca
     5701 aggctggcgg ccgccggtgt gatccggcgc aagcgtgtaa agcccatatg acacctcctt
     5761 gtacagttat cgtggatggc acatcataac atatacggtg gctaaccggc aaactcaagg
     5821 cgatagcgcg cgatccaccc tccgtccggg cttgtgacgc tctcgaagtt ctggtagtat
     5881 gccgtccgtc gccgtcattc tcagcctgga tttcagatcg tcgtgtcatc tcgtcgccat
     5941 cctccggcgc aggccgccgg gatcgttctc ctgctcgtgc tggcttattt gcagttcagc
     6001 acgctcgccg tcacctcgcc gacgctggac gagccgaacc acctggtcag cggttacgcc
     6061 tttctcacgc gtggcgatac gcgcatcaag ctcaacggcc ccatcctgcc taacgcactg
     6121 ggcgcgctgc cgctcctgct tcagcccggt ctggtgctgt cgccgccgga cgatccgggt
     6181 tgggacacga accagtacaa cgacatcgcc gatcggttcc tgtggagcaa cacggcatcg
     6241 ccgtttcaca tcatttacct ggcgcgcttt ccgttcatgg ccgccggctg gctgctcggc
     6301 gcgctcatct tccgctgggc gcgcgagcga agcggaccgc gggccggatt atttgcgctg
     6361 gccttctacg tgttctgccc gaatctgctg gcccactcac ggttcgtcat gaccgacttc
     6421 gtgccggctg cactcacctg cctgacgctc tacaccttcg atcgatcgat gcattcggat
     6481 cgccccggct gggcgatcgc cgccggcact gcgctgggat tggcgctcgc atcgaagttt
     6541 tcgttggtca tgctcatccc cgctatggcc atccttctct tcctgcgcgc cgggtggcaa
     6601 agcgtgcggc gcccgctggg gcagctcgcg ctgaccgtcc tcgtcgccgc cgtcgccata
     6661 tggggcgtgt acagttttca agttgcgccg gtctcggccg gtggtcttcc ggtgcccgct
     6721 cctggcttct ggggcgaagt ggaatccgcc cgctatgttc tcggccagcc gtggcccaat
     6781 tatctgttcg gccagatatc cgacagtggc tggtggtatt actttccaat cgcgttcctc
     6841 gtcaaaacgc cgctgcctat gctgatcctg ttcgcagccg cgctcgtccg cacggttcgc
     6901 gcccgatcgt ggcggcgcga tgctgtcttc ttactgccct cgtttctgtt ggtgttcggc
     6961 ctgctcttca gcaccaataa cctcggctat cgctacctgc ttccgctgtt gcctctgatc
     7021 tttgtgtatg cctccgacct cgttcccaag agttccttcg ctctgtctcc ccgccatctt
     7081 gtcactctgt cactactcgc ctggcacatt atcggcactt tgcgaatcta tcctttctat
     7141 ctcaccttct tcaacgagat cgccggtggc ccagatcgcg ggcgatacgt cctgtccgac
     7201 tcgaacatcg actgggggca ggacatggcc gggctgaagc agtacgtcga agaaaatggc
     7261 atcgaccgga tcaagctgag ctatttcggg attgcgcacc cgacggccta tggcctgaag
     7321 gccgagccgc tgctgcccgt ccgcaccgcg atgcacgacc agggcgcgtg gtggctgcac
     7381 accttctacc cgcacgatcc gcctccgggc gtttatgcga tcagcgtcgc caacctgatg
     7441 ggcggcatct ggatcgatcg cgaaacctac gcctatttcc gagatcgcaa accggacacc
     7501 acgatcggca acgcgatcta catcttcacg atctcgccgc gcggcgagcc agttgacctg
     7561 tcgctcgccg gcctgcagat cgaccagatc gacccggaca cctacacgcg cttcggcacg
     7621 aacgacgtgc gcccgcgctg gttcgacgag acgacttcgc taatcgccgc gccgggcgag
     7681 tcctgggtcg ctatctccga tctccagtct ctaccgcccg agttcgccgt tctgcttgat
     7741 gacgtgcagc ccatcgctcg gccgcgcacg gtcgacgacg gccaacctta tcgcctgtat
     7801 cgcctggatc ttgcccggcg catgcgcggc gcagccgccc aggccgagca agtcgtgcag
     7861 gagggagtct tgcccgcgac gttcggtcgc acggctgaat tgatcggcta cagcctggcg
     7921 gaggattcag aacggtcacc aggcatcacg ctcgtcacct actggcgtgc gggcgatgat
     7981 gtggtgacgc cgctgcagtt attcgttcac gccgtggatg cgagcgatca g
//
LOCUS       DBNHXB010000309        11900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2934443, whole genome shotgun sequence.
ACCESSION   DBNHXB010000309 DBNHXB010000000
VERSION     DBNHXB010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11900
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2934443"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2474)
                     /locus_tag="AC162G_12440"
     CDS             complement(<1..2474)
                     /locus_tag="AC162G_12440"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase, EACC1-associated type"
                     /protein_id="HIR5076674.1"
                     /translation="MADARVALIVASDQYDDPGLRQLVAPPQDAEALARVLGDAAIGG
                     FQVRTLLNEPSYRVSQEIEAFFADLKRDDLVLLYFSGHGVKDEDGQLYFAMPDTRRNL
                     LRSTAVSSSLVNDVMRRSRSRQQVLVLDCCYSGAFARGMIARGSSDIGTKERFEGQGR
                     VVLTASDAMQYSFEGDVVKGEGISSIFTRTFVRGLETGEADRDGDGQITIDEIYDYVH
                     DRVCELTPKQRPGKWEFDVQSDLVIARNPRPLPKPAALPVEVQQAIESPLASVREGVT
                     RELAQFLRGSHAGLALAAHEALQRLTDDDSRRVADAARRALATYAESQRAGWIGYTFN
                     NRYRIEALLGQGGMSAVYRGVDLNLRRPVAIKLIHPHLSSDPEFVRRFEMEATAVARL
                     RHPNIMQVFDFDHEGDVYYMVLVYLEGETLQARLKTLDEAQARLPLAETIRLTALICD
                     AVAYAHREGIVHRDLKPANVMLDPHDQPILMDFGVAKMLGGQKHTATGAIVGTVAYMS
                     PEQLRGDPFDGRADIYSLGVMLYEMTAGCPPFGADSVMTLMLKHLNEPVPDIREFAAN
                     VPDELVVAIEKALAKTPGERFQTAAEMAEALRAIDLARIERERQAKERVEAERAAKQK
                     AEQERLVRERAEQERRAQEKAEQERRARERAEQERRAKEKAEAERAAREEAEWRARQK
                     AEEARLAQERAEQERLATEKAEKERLARERAEAERLARQKAEQERLAREKAERERRKR
                     EIEGEAQPRPLPAWAGNRLVWLIGGAAAVVILGVLAVVPLLRPALPSGAGMVAIPGGS
                     YSVGLDPSAREFQDSNYVSSLSVELSS"
     gene            complement(2884..3714)
                     /locus_tag="AC162G_12445"
     CDS             complement(2884..3714)
                     /locus_tag="AC162G_12445"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5076675.1"
                     /translation="MEDLTGRKIGSYQIIAPLATGSMAMVYKALQPTLRRHVALKVLP
                     RHLASEPTFVGRFHQEARIIASFQHPNILPLYDFGEVDDYTYMVMPLIETGTLANRLQ
                     GQPLPLEEIRLVGAQVGDALEYAHSLGVVHRDVKPSNILINRRGTCLLSDFGIAKLIE
                     EAVKFTATGETVGTPAYMSPEQGLAQKVDRRSDIYSLGVILFQMSTGRLPFEGKTPVA
                     TVLMQVNEPLPLPRSLNPDLSIELEEVITRALAKAPDDRYATAAALVRALYAATSARG
                     "
     gene            complement(3771..4643)
                     /locus_tag="AC162G_12450"
     CDS             complement(3771..4643)
                     /locus_tag="AC162G_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076676.1"
                     /translation="MAAPGRAMRTTRLTPGEFYRRHVQRKKMIALIAGLLLLPVSLLL
                     IVSFDRGALGRAVSEALGFPFAGGALPPPEPATVVVAQAPTPAPCLDTVFNGVALDAE
                     TDGLLGGVRVTASFGASAETSPDGEYKILVCYDLEVHDQFSLVFEKEGYEPASLDVVT
                     KGYPGLSFRVADVAMASIPAETPVPTLFQPTRQTPLPTPTPTLSQPPRQTPLPTATSL
                     AARSGAAATPAPTRAAGSPAPTGTPGAAPPSTLPVTGVPEIAFPGQAIGFVLLAISLL
                     LIAAGVWPDPKRTR"
     gene            complement(4647..5903)
                     /locus_tag="AC162G_12455"
     CDS             complement(4647..5903)
                     /locus_tag="AC162G_12455"
                     /inference="COORDINATES: protein motif:HMM:NF025873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR5076677.1"
                     /translation="MDLSTIVAIVFGLILGFLIGWIIEWRMDLAFWKARAAQFDPSGD
                     MAETATSVDSVTTTFKELLDTRDAELASLRAQLAERDQQHLAELADHDRQAQARFDER
                     EATLRAEFDRREAAARAQAQEQLTAARTQLEQQLAALRGQSEALAASLRGEYESREAA
                     RLQDFEQRLAEMRAAHESHEAVRRAEFDERLSAAGAERDARAAELQRQYEAKLAEARA
                     DMENRVAAAIQDREERLAALRSELESRTAGAVQEREERQAAAQSQYEARLADLRAELD
                     HRTATIKGHDERIAALQSEYATQLAELRRDDEKREQARRQEFEARLSEVERARAVVES
                     DLKFIRRRYEVYVRHHPDDLMAIKGIGPKTEWLLHKAGINSFAELAEATPDEIRSVLN
                     APRWRRIDPGAWIKQAKTLAKYGEKE"
     gene            complement(5934..6533)
                     /gene="scpB"
                     /locus_tag="AC162G_12460"
     CDS             complement(5934..6533)
                     /gene="scpB"
                     /locus_tag="AC162G_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR5076678.1"
                     /translation="MSESNVEPMSDELPLPLTGMVESLLFVSGEPVSVARLAEALEVS
                     AEQVEEALAELREATRNGASRGLILQRRGDKVQLTTRPECAPYVEKFLGLSLTSRLSK
                     PALETLAIVAYQQPVTRPEIEMIRGVNCDGVIQTLLSKGLIEEVGRLETAGRPIQYGT
                     TFAFLQHFGLRSLEDMPPLRTGEPAAASEAQPGELAAQP"
     gene            6752..7315
                     /locus_tag="AC162G_12465"
     CDS             6752..7315
                     /locus_tag="AC162G_12465"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5076679.1"
                     /translation="MQIVALSPDNEDYIDQAAVLLVEGFKQHWPDAWPDMASALQEVR
                     EALGPGRIAHIAVDDDGSVLGWIGGIEQYDGHVWELHPLVVRPDRQRQGIGRALVADL
                     EDRVRERGGLTVTLGTDDEDSMTTLSGVDLYPNVWEHIARVRNLRGHPYEFYQKMGYV
                     ITGVMPDANGLGKPDILMSKRVAKGQP"
     gene            7312..8367
                     /locus_tag="AC162G_12470"
     CDS             7312..8367
                     /locus_tag="AC162G_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR5076680.1"
                     /translation="MSERSGNLSSADHDQARHEPDHDHPSGLRGRLAGLFGGHTHDVQ
                     AAALDPALWSRRGIRAVKVSLAGLMLTALFQVAIVAMSGSVALLADTIHNFSDALTSI
                     PLWLAFSLSRRERNRRYTYGYGRAEDLAGAFVVLVIFASALIVLVESIQKMIHPQPIA
                     HLGWVAAAAIIGFAGNEIVALFRIRIGRQINSTALVADGLHSRADGFTSLGVLAGALG
                     VALGFPVADPLVGIAIGIVILGVAWGAAREIGYRLMDAVDPAVCDRIESTTRGVAGVM
                     GVHEVAVRWVGHRQRAEVHIVVDGKIPTSESHQVAEAVRHALFHALPALVDVTVHIDP
                     SENASGTTHKTTEHHHP"
     gene            8518..9792
                     /locus_tag="AC162G_12475"
     CDS             8518..9792
                     /locus_tag="AC162G_12475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019506009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5076681.1"
                     /translation="MQITILTAGTRGDVQPYVALGLGLKRAGHAVRLATHAIFRDFVA
                     SHGLDFAPVEGNPKEILSGAKGQELLESGRNSLAFLRHGLRLLRPVLERMLADSIAAC
                     EGAEAIISPFAFGGYHVAEKMGVPCVGSALIPLARTRAFPNIQVHPRWKLGGTFNYLT
                     HALGDQALWTALRQPINAWRREALSLPPIGFGGPMRVMHQKRMPYLYGYSQHVVPKPP
                     DWGEWLHVTGYWFLDSATEWQPPNNLHSFLSDGPPPVYVGFGSMTPRQVEFLTTTAID
                     ALKRAGQRGILLTGWGGIGQSELPDTVLKIDAVPHEWLFPRMAAIVHHGGAGTTAEAV
                     RAGVPSIVTPFFADQPFWGQRIADLGVGPAPIPQKQLTTQKLAEAIHIAVTDASMRDR
                     AAALGQKIRAEDGVARAVEAFHQHVRIREVMR"
     gene            complement(9802..>11900)
                     /locus_tag="AC162G_12480"
     CDS             complement(9802..>11900)
                     /locus_tag="AC162G_12480"
                     /inference="COORDINATES: protein motif:HMM:NF021126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIR5076682.1"
                     /translation="GVAAALAAVQLLPTLELAGLSPRQGGLPLDEAVSFSLDPRLLGR
                     ALLPDYDGALPGGSEFTAFLGVSTLVVMIIGLLAAWSHGATAWQPIGALITDRLTYRS
                     LSVLAFLGIFLALGGFNPLYYLLVKFVPGFDLFRAPARWLALFVFGGSMLAAVGLDRF
                     GRGLTRITRIGIAGVMALLIGTTFISANVTPAGAAGPIGVPGIESLALWLGAALLTLL
                     LATRLQPPTSNLQSLILLLCALELFIATRNLPYHTRLTAPDAWTSLRPPVAQLLVGAS
                     GAPAGRFLSISDIQFDPGDAAELESIYADQLPPDAYYDLLIATKQKEIVAPNQPLTYG
                     LPAVDGYDGGLLPLRNYLTFQKLFLPGDAIQPDGRLREQLESIPDARWLDLMNVKYVI
                     TDKVRDRWFDGVFFDLQFETPLRPGDKVWTDQLPPLEANALGIVYSEPKGEVTLAEVE
                     ITFEDGSSATAPLADEPIERADDLSMTRLRWEEAQRVSRVEVVGRGGLTLHGVALIDE
                     RSDTFHSFALAKDGRFRLAHSGDVKVYEYVDALPRAFVVPNSVSAASDDEALALMQNP
                     DFDPSKTVVIHEAMPPATRGELQITNYELRFTNYESRFTDYAPEHIEMEVKADGDGYL
                     VLTDAWYPGWIATVDGEAVDILRADIAFRAVPIEAGEHRVEFRYEPQAFRVGALISGG
                     ALMLVVAALAAARING"
BASE COUNT         2069 a         3921 c         3810 g         2100 t
ORIGIN      
        1 gacgagagtt ccaccgacag agaggacacg tagttgctgt cctggaactc acgcgccgat
       61 gggtcgagcc cgacactgta cgacccaccg ggaattgcga ccatgcctgc gcccgaaggc
      121 agcgcagggc gcaacagcgg gacgacggcc agcacaccca ggatgacgac cgccgcagcc
      181 ccgccgatca accacaccag ccgattcccc gcccaggccg gaagcggccg tggctgtgcc
      241 tcaccctcga tttcgcgttt gcggcgctcc cgctcggcct tctcgcgggc cagccgttcc
      301 tgttcggctt tctgcctcgc caggcgctcg gcttcggcgc gctcgcgggc cagccgctct
      361 ttttcggcct tctccgtcgc caggcgttcc tgctcagctc gttcctgcgc cagccgtgcc
      421 tcctcggctt tctgccgggc acgccactcg gcttcttcgc gcgccgcacg ttcggcctcg
      481 gccttctctt tcgcccggcg ttcttgctcg gcgcgttcgc gtgcccgccg ctcctgttca
      541 gccttctcct gcgcccggcg ttcttgctcg gcccgttcgc gcacgagccg ctcttgctcg
      601 gctttctgct tggccgcgcg ttccgcctcg accctctctt tggcctgccg ttcccgctcg
      661 atgcgcgcca gatcgattgc ccgcagcgcc tcggccatct cggcggcagt ctgaaaacgc
      721 tcccctgggg tctttgccag cgccttctcg atcgccacaa ccaactcgtc cggcacattt
      781 gcggcgaact cgcggatgtc cggcacaggc tcgttgagat gcttgagcat cagcgtcatg
      841 accgaatcgg caccgaaagg cgggcagccg gcggtcatct cgtagagcat tacgccgagc
      901 gagtagatgt ctgcgcgccc gtcgaacgga tcgccgcgca actgctccgg cgacatgtag
      961 gccacggtgc cgacgatcgc gccggtcgcg gtatgcttct gcccgcccag catcttcgcc
     1021 acgccgaagt ccatcaggat cggttgatca tgcggatcga gcatcacgtt cgccggcttc
     1081 aggtcgcggt gaacgatccc ctcccggtgc gcgtaagcga ccgcgtcgca gatgagagcc
     1141 gtgagccgga tggtctctgc cagcggcaag cgtgcctggg cttcgtctag cgtcttcagc
     1201 cgggcctgga gtgtctcgcc ttccaggtac accagcacca tgtaatagac gtcgccctcg
     1261 tggtcgaagt cgaagacctg catgatgtta gggtggcgca ggcgggcaac ggcggtggcc
     1321 tccatctcga aacgccgcac gaactcggga tcactggaga ggtgcggatg gatcagcttg
     1381 atcgccaccg gccggcgcag gttgaggtcg acgcctctgt acacagccga catgccgccc
     1441 tgcccgagca aggcttcgat acggtaccgg ttgttaaacg tgtaaccaat ccagcccgcg
     1501 cgctgcgact cggcgtatgt cgccagcgcg cgccgggcgg cgtcggccac gcggcgactg
     1561 tcgtcgtcgg tcaggcgctg gagcgcttcg tgcgcggcca gcgccaaccc agcgtggctg
     1621 ccgcgaagga actgcgccag ctcgcgggtc acgccctcac gcacgctggc gagagggctt
     1681 tcgatagcct gctgcacctc gaccggcagc gcagcgggct tggggagcgg ccgcggattg
     1741 cgggcgatga cgaggtcgct ctgcacgtcg aactcccact tgccgggtcg ctgcttgggc
     1801 gtcaactcgc agacacggtc gtggacgtaa tcgtaaatct cgtcgatcgt gatttgaccg
     1861 tcgccgtcgc ggtcggcttc gcccgtctcc agcccgcgca cgaaagtgcg cgtgaagatc
     1921 gagctgatcc cctcgccctt caccacgtct ccttcgaagg aatactgcat cgcgtcagac
     1981 gccgtcaaga cgacgcgccc ttgaccttcg aaccgttcct tggtgccgat gtcgctgctg
     2041 ccgcgcgcga tcatgccgcg cgcgaacgcc ccgctgtagc agcagtcgag caccagcacc
     2101 tgctgccgcg agcggctgcg gcgcatgacg tcgttgacga gcgaggagga cacggccgtc
     2161 gagcggagca ggttgcgccg cgtgtcgggc atggcgaagt acaactggcc gtcttcgtcc
     2221 ttgacgccgt ggccggaaaa atacagaagc accaggtcgt cgcgcttgag atcggcgaag
     2281 aaggcttcga tctcctggct gacccgatac gatggctcgt tcaagagcgt gcgcacctgg
     2341 aagccgccga tggccgcatc gcccagcaca cgcgcaagcg cctcggcatc ctgtggcggg
     2401 gcgacgagtt gccgcaggcc gggatcgtcg tattggtcgc ttgcgacaat caacgcgacg
     2461 cgtgcgtcag ccatcttcca tgaatccctg gcgtgctacg aatgcgctgc ttgacaaatg
     2521 atgccaactc catgcttcca caagaaatgg gcgcaatcga tcccaatgtc ataaacgaca
     2581 aatatgcgcg tttttcagca ccatccacag cggatagggg cgcttattcg agggtgcacc
     2641 ccttggcttg aaccttattc tattggttat cggcgctgag gtcaaatcaa cacgcgccga
     2701 accgggacac gaactcccgt tcggcgcgcg catcaatgtt actctggagt cgaaaatact
     2761 cacgaggcat ctccaccctg gtgcgtggtg ttcgccggtc ggagcagaga ttcctcgctg
     2821 cgctcggaat gacacttttg aagtttggag tttgaagttt ggagcttgag cgacctcttg
     2881 cgcctaaccc cgcgccgacg tcgccgcgta cagcgcacgc accagcgccg ccgccgtcgc
     2941 gtacctgtca tcgggagctt tggccagcgc ccgggtgatg acttcttcaa gctcgatcga
     3001 gagatccgga ttcaacgagc gggggagggg cagcggctcg ttgacctgca tcagcacggt
     3061 tgccaccggc gtcttgccct cgaagggcag gcggccggtc gacatctgga acaggatgac
     3121 gcccagcgag tagatgtcgc tgcgccgatc gaccttctgc gccaggccct gctcgggcga
     3181 catgtaagcg ggcgtgccga ccgtctcgcc cgtcgccgtg aacttcaccg cctcttcgat
     3241 cagcttcgcg atgccgaagt ccgaaagcag gcacgtgccg cgcctgttga tcaagatgtt
     3301 gctcggcttg acatcgcgat ggacgacgcc gagtgaatgg gcgtactcga gcgcgtcgcc
     3361 aacctgcgcc cctaccaggc gaatctcttc gagtggcagc ggttggcctt gcaggcggtt
     3421 ggccagcgtg cctgtctcga tcagcggcat gaccatgtac gtgtagtcgt ccacctcgcc
     3481 gaagtcgtaa agaggcagaa tattggggtg ctggaagctg gcgatgatgc gggcttcctg
     3541 gtgaaaccgc ccgacgaacg tcggctcgct ggccaggtgg cgcggcaata ccttcagcgc
     3601 gacgtggcgc cggagcgtcg gctgaagcgc cttgtacacc atggccatgc tgcccgtggc
     3661 gagcggcgcg atgatctgat acgagccgat ctttctgccg gtgagatcct ccatcgagcc
     3721 tcgcgctacg gttggtcgcc tgtggagcgt atgccgatct tcatggtgac ctagcgggtg
     3781 cgcttagggt ccggccacac gccggcggcg atgagcaaca gcgagattgc cagcagcacg
     3841 aatccgatcg cctggccggg aaaagcgatc tccggcacgc cggttacagg caacgtgcta
     3901 ggaggagcgg cgccgggcgt gccagtcgga gccggactgc ccgctgcccg cgttggcgcc
     3961 ggcgtcgccg cagcgcctga tctggcggcc agggatgtgg ccgttggcag cggcgtctgg
     4021 cgcggcggct gagacagcgt cggggtcggc gtcggtaggg gcgtctggcg cgtcggctgg
     4081 aatagcgtcg gaaccggtgt ctcagccggg atggaagcca tcgccacgtc ggcgacccgg
     4141 aagctgaggc caggatagcc cttcgtcacg acgtccaggc tggccggctc gtagccctcc
     4201 ttctcgaaga cgagcgagaa ttgatcgtgc acttcgaggt cgtagcacac caggattttg
     4261 tactcgccgt caggcgacgt ttccgcgctc gcgccgaacg aggccgtcac gcgcacccca
     4321 cccagcagcc cgtctgtctc ggcatcaagc gccacgccat tgaagacggt gtcgaggcaa
     4381 ggcgcaggcg tgggagcctg ggccacgacg accgtggccg gttctggcgg cggtaatgca
     4441 ccgccagcga acgggaatcc caacgcctcc gagaccgccc gccccagcgc tccgcgatcg
     4501 aacgacacaa ttagcaggag cgacacgggc agcagcaaca agccggcgat cagcgcgatc
     4561 attttcttgc gctgaacgtg acgtcgatag aattcgcccg gcgtcaaccg ggtagtccgc
     4621 atcgcgcgac ctggggctgc catggctcac tccttctcgc cgtacttcgc cagcgtcttg
     4681 gcctgcttga tccacgcccc gggatcgatg cgccgccacc tgggcgcatt gaggacgctg
     4741 cggatctcgt cgggcgtcgc ctccgccagt tcggcgaagc tgttgatgcc cgccttgtgc
     4801 agcagccact cggtcttcgg accgatgccc ttgatggcca tcagatcgtc ggggtgatgg
     4861 cgcacgtaca cctcgtagcg cctgcggatg aacttgagat ctgattcgac gacggcccgc
     4921 gcgcgctcca cttcggacag acgcgcctcg aattcttgtc gccgcgcctg ttcgcgtttc
     4981 tcgtcatcac ggcgcagctc ggccagttga gtcgcatact cggactgcag cgcggcgatc
     5041 cgctcgtcgt gccccttgat ggtcgccgtg cgatgatcaa gctcagcgcg caggtcggcc
     5101 agacgcgcct cgtattggct ctgcgccgca gcctggcgct cttcgcgttc ctgcacggct
     5161 cctgccgtcc gactctccag ctccgaccgg agcgcggcaa ggcgctcctc gcggtcctgg
     5221 atcgcggccg ccacgcggtt ctccatgtca gcccgagcct ccgccagctt tgcctcatac
     5281 tgacgctgca gctctgccgc ccgtgcatcg cgttccgcac ccgccgccga cagccgttcg
     5341 tcgaattcgg cgcggcgcac tgcctcatgc gattcgtgcg ccgcgcgcat ctcggccagg
     5401 cgctgctcga agtcctgcag gcgggccgct tcgcgcgatt cgtattcgcc gcgcagtgag
     5461 gcggccagcg cctcgctctg gccgcgcagc gccgccaact gctgctcgag ctgcgtccgc
     5521 gcggcagtga gctgttcctg agcctgcgcg cgcgccgccg cctcacgccg gtcgaactcg
     5581 gcgcgcagcg tggcctcgcg ctcgtcgaag cgcgcctgtg cctgcctgtc atgatcggcc
     5641 agttcagcca ggtgctgctg atcgcgctcc gccagttgtg cgcggagcga ggcgagttcg
     5701 gcgtcgcgcg tgtccagcaa ctccttgaac gtggtggtga ccgagtcgac cgaggtcgcc
     5761 gtctcggcca tgtcgccgct cgggtcgaac tgcgcagcgc gcgctttcca aaacgccagg
     5821 tccatgcgcc actcgatgat ccagccgatc aggaatccca agatcagtcc gaatacgatt
     5881 gcgacgatgg tcgataggtc cacgcagtac tccttttcga ggcatgcggc gcgctaaggc
     5941 tgcgccgcca attcgcccgg ttgggcttcg gacgcggctg caggctcgcc ggttctaagc
     6001 ggcggcatgt cctccaggct gcgcagcccg aaatgctgca aaaacgcgaa agtcgtgccg
     6061 tactggattg ggcggcctgc tgtctccagg cggccaactt cctcgatcag tcccttgctc
     6121 agcagggtct ggatcacgcc gtcgcaattg acgcctcgga tcatctcgat ttcggggcgg
     6181 gtgacgggct gctggtaggc aacgatggcc agcgtctcca gcgccggctt ggacaggcgg
     6241 ctggtcagcg atagaccgag gaacttctcg acatacggcg cgcattcggg ccgcgtggtc
     6301 aattgcacct tgtcgccccg gcgctgcagg atcagcccgc gcgacgcacc gttccgcgtc
     6361 gcctccctca gctcggcgag cgcctcctca acttgctcgg cgctcacctc cagcgcttcg
     6421 gcaaggcgcg ccacggacac cggctcgccg gacacaaaga gcaggctctc gaccatcccc
     6481 gtcagcggca ggggcaattc atctgacata ggctcgacgt tcgattccga catgatccgc
     6541 gccgcttcgc tccgccaaag agaatgccta acgagattat agcatagcga gaaggttgca
     6601 tgaaaccggc gctcgcgcca aagctctcga atccggtata atgtgctccg gcgtgtatcg
     6661 cccgcgttca cgatgttgta gtaacgactt cagtcgtccg aataaagcag cgactgaagt
     6721 cgccactact gggtcttgac tgaaacgaac cgtgcagatc gtcgctctct ctcccgacaa
     6781 cgaagattac atcgatcagg ccgccgtgct tctggtcgag ggcttcaagc agcactggcc
     6841 ggatgcgtgg cccgacatgg cttcggccct gcaggaagtg cgcgaggcgc tcggccctgg
     6901 ccggatcgcc cacatcgccg tcgacgacga cgggagcgtg ctgggctgga tcggcgggat
     6961 cgagcaatac gacggccacg tgtgggagct gcatcccctg gtcgtccgcc ccgatcgcca
     7021 gcgccagggc atcgggcgcg cgctggtagc cgatctggaa gatcgggtgc gcgagcgcgg
     7081 cggcttgact gtcaccctcg gcacggacga cgaagacagc atgaccacgc tgtccggcgt
     7141 ggatctgtat ccgaacgtgt gggagcacat cgcccgcgtc cgcaacctgc gcggccatcc
     7201 ttacgagttc taccagaaga tgggatacgt gatcacgggc gtcatgcccg atgccaacgg
     7261 cctcgggaaa cctgacatcc tgatgtcgaa gcgtgtggcg aaggggcagc cgtgagcgaa
     7321 cggagcggca acctgtcgag cgccgaccac gaccaggcac ggcacgagcc ggatcacgat
     7381 cacccgtcgg ggctgcgggg ccggctcgcc ggcctgttcg gaggacacac acacgacgtc
     7441 caggcggcag cgctcgatcc ggcgctgtgg agccggcgtg gcatccgggc ggtcaaggtc
     7501 tcgctcgctg gcctgatgct gaccgcgctg ttccaggtcg ccatcgtcgc catgagcggc
     7561 agtgtggctc tgcttgccga caccattcac aacttctccg acgcgctgac gtcgatcccg
     7621 ctgtggctcg ccttcagcct gtcgcggcgc gaacggaacc ggcgttacac gtacggttac
     7681 ggtcgcgccg aagatctggc cggcgcgttc gtcgtgctgg tgatcttcgc cagcgcgctg
     7741 atcgtgctgg tcgaatcgat ccagaagatg atccatccac agccgatcgc gcatctcggc
     7801 tgggtcgcgg cggcggcgat aatcggcttc gccggcaacg agatagtcgc tctctttcgc
     7861 atccgtattg ggcgacagat caactcgacg gcgctcgttg cggacgggct gcactcgcgc
     7921 gccgacggct tcacctcgct cggcgtcctc gccggcgcgc tcggcgtcgc gctcggattt
     7981 cccgtcgccg atcccctggt ggggatcgcg atcggcattg tcatcctggg cgtcgcctgg
     8041 ggtgcggcgc gggagatcgg ctatcgtctg atggatgccg tcgatccggc ggtatgcgat
     8101 cggatcgaaa gcacgacgcg cggcgtagca ggcgtgatgg gcgtgcacga ggtcgccgtg
     8161 cgatgggtgg gccaccggca acgggccgag gtgcatatcg tcgtcgacgg gaagataccg
     8221 acgtccgaaa gccatcaggt tgcggaagcg gtacgccatg ccctgtttca cgcgctgccg
     8281 gcgctggtcg acgtcacggt gcacatcgat cccagcgaaa acgcatcggg cacgacgcac
     8341 aagacgactg agcatcatca cccgtgagag cgcatcttaa aacccgaaga acgcgcatca
     8401 aaacccgtag aacgcgcatc aaaacccgtg gagcgggcat ccctatgccc gctgttgacg
     8461 catggtgatg cgtcaaccac gggatttaag acagtccgtc gggctgagac aaccggcatg
     8521 cagatcacga ttctgacggc aggaacgcgc ggcgacgtgc agccctacgt ggcgctcggc
     8581 ctcgggctga aacgggccgg gcacgccgtg cgccttgcga cgcacgcgat tttccgcgat
     8641 ttcgtcgcga gccacggcct cgacttcgcg cccgtcgaag gcaacccaaa ggaaatcttg
     8701 tccggcgcga agggacagga attgttggaa tcgggccgca attccctcgc tttcctccgg
     8761 catggtctgc ggctgctgcg cccggtgctg gagcgcatgc tcgccgacag catcgccgcc
     8821 tgcgaagggg cagaggcgat catctcgcca ttcgcgttcg gcggctatca cgtcgccgag
     8881 aaaatgggcg tgccttgcgt cggcagcgcg cttataccgc tagcgcgcac ccgagcgttt
     8941 cccaatattc aggtgcatcc ccgctggaag ctgggcggca cgtttaacta tctgacgcac
     9001 gccctcggcg accaagccct ctggacggcg ctgcgccagc cgatcaacgc gtggcgacgc
     9061 gaggcgctga gcctgccgcc gatcgggttc ggcggcccga tgcgcgtcat gcaccagaag
     9121 cgcatgccgt atctgtacgg ctacagccag cacgtggtgc ccaagccgcc ggattggggc
     9181 gagtggctgc acgtaactgg atactggttt ctcgacagcg cgaccgagtg gcagccaccg
     9241 aacaacttgc acagttttct atccgatggg ccgccgcccg tatacgtcgg cttcggaagc
     9301 atgacgcctc gccaagtcga attcctgacg acgacggcga tcgacgcact gaagcgggct
     9361 gggcagcgcg gcatcctgct caccggctgg ggtgggatcg gccagagcga actgccggac
     9421 accgtgctca agatcgatgc cgtcccgcac gagtggctgt tcccgcgcat ggcggccatc
     9481 gtgcatcacg gcggcgcagg cacgacggcc gaggccgtgc gcgccggggt tccgtcaatc
     9541 gtcacaccgt tcttcgccga tcagccgttc tgggggcagc gtatagcaga cctcggcgta
     9601 gggccggcgc cgattccgca gaagcagctc acaacccaga agctggcgga ggcgatccac
     9661 atcgccgtca cggacgcaag catgcgggat cgcgctgccg cgctcgggca gaagatccgc
     9721 gcagaggacg gggtcgcgcg agcagtcgaa gcgtttcatc aacatgtcag aataagagaa
     9781 gtcatgaggt aagcgttgtg ctcaaccatt gatgcgggcc gccgcaagcg ccgccaccac
     9841 aagcatgagc gcacccccac tgatcaacgc cccaacgcgg aacgcctgtg gctcgtagcg
     9901 aaactcgacc cggtgctcgc ccgcctcgat cggcacggcg cggaaggcaa tgtcggcgcg
     9961 gagaatgtcg accgcttcac catccacggt cgcaatccaa ccgggatacc aggcatcggt
    10021 caggacgagg tagccgtcgc cgtctgcctt gacttccatt tcgatatgtt ctggcgcgta
    10081 atctgtgaaa cgtgattcgt aattcgtaaa acgtaattcg taatttgtaa tttgtaattc
    10141 gccacgggtt gcaggcggca tagcttcgtg gataaccaca gtcttcgatg gatcaaagtc
    10201 aggattctgc atgagcgcga gggcttcgtc gtcgctcgcg gcggagacac tattcgggac
    10261 gacaaaggcg cgcgggagcg cgtcgacgta ttcgtagacc ttcacgtcgc cggaatgggc
    10321 gagccggaag cggccgtctt tcgccagggc gaacgagtgg aacgtgtcgc tgcgctcgtc
    10381 gatcagcgcc acgccgtgta gtgtcagacc gcctcgcccc actacctcca ctctcgacac
    10441 acgctgcgcc tcctcccagc gcaggcgcgt catggatagg tcgtctgccc gctcgatcgg
    10501 ctcatcggcg agcggcgcag tcgccgacga gccatcctcg aacgtgattt ccacctcggc
    10561 cagggtcact tcacccttcg gctctgagta gacgatgccc agcgcatttg cctcgagcgg
    10621 cggaagctga tccgtccaca ccttatcgcc cggcctcaaa ggcgtttcga attgcaggtc
    10681 gaagaacacg ccgtcgaacc agcggtcgcg caccttgtcg gtgatgacgt acttcacgtt
    10741 catcagatcg agccagcgcg catccgggat cgattccagt tgctcgcgca ggcggccatc
    10801 gggctgaatg gcgtcgcccg gcaggaacag tttctggaag gtcaggtagt ttcgcagcgg
    10861 caacaacccg ccgtcgtatc cgtccacggc aggcaagccg taggtgagtg gctggttcgg
    10921 cgcgacgatt tccttttgct tcgtcgcgat gaggagatcg tagtaggcgt ccggcggaag
    10981 ctgatccgcg tagatcgact ccagctcggc ggcgtcgccc ggatcgaact gaatgtccga
    11041 aatcgacagg aagcgccccg ccggcgcgcc gcttgcgccc acgagcaact gcgcaacagg
    11101 cgggcgcagg ctggtccacg cgtcgggcgc ggtcaggcgg gtgtggtatg gcaggttgcg
    11161 ggtagcgatg aagagttcga gggcgcacag caagagaatt agagattgga ggttggaggt
    11221 tggaggttgg aggcgggttg ccagcaacag tgtcaacaag gctgcaccca gccacaacgc
    11281 gagcgattcg atgccaggta cgccgatagg cccggcagca ccagcgggcg tcacgtttgc
    11341 actaatgaac gttgtgccga ttaacagggc catcaccccg gcaatcccaa tccgcgttat
    11401 ccgcgttaat ccacgtccaa aacgatccag acccacggcg gccagcatcg accccccgaa
    11461 gacgaacagc gccagccagc gcgcgggtgc tcggaagaga tcgaatccgg gaacgaactt
    11521 cacgagcaga taatagagcg gattgaagcc gccgagtgcg aggaaaatcc ccagaaaggc
    11581 gagaacggac agtgaacggt aagtcagtcg gtctgtgatc agtgcgccaa ttggttgcca
    11641 ggctgtcgcg ccgtgcgacc aagcagccaa caagccaatg atcatcacca cgagcgtaga
    11701 gacgccgagg aatgccgtga actcgctccc gcccggcagc gcgccatcgt agtcgggcaa
    11761 tagcgcgcgg ccgagcaggc gcggatcgag cgagaacgac acagcctcgt cgagcggcag
    11821 cccgccctgg cgcggagaaa gccccgcaag ctcgagcgtc ggcaggagct gaacggcggc
    11881 cagcgcggcg gcgacgccca
//
LOCUS       DBNHXB010000310         6282 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_754713, whole genome shotgun sequence.
ACCESSION   DBNHXB010000310 DBNHXB010000000
VERSION     DBNHXB010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_754713"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="AC162G_12485"
     CDS             <1..253
                     /locus_tag="AC162G_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HIR5076683.1"
                     /translation="GTVVATRKYTGLEGVKFLIVQPLDKRQQPDGDPLVAADATAQAG
                     PGHLVFVVGVREAALALPVKFVPVDHAIVGIVDQVDTVP"
     gene            320..592
                     /locus_tag="AC162G_12490"
     CDS             320..592
                     /locus_tag="AC162G_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HIR5076684.1"
                     /translation="MYIGRVFGTVVATIKHETFANRKLLLVERLSPEGEPDKKYDVAV
                     DVVQAGVGDRVLVLDEGNSARQIVDLEPDGAIRAVVVGIVDEIDLS"
     gene            775..1593
                     /locus_tag="AC162G_12495"
     CDS             775..1593
                     /locus_tag="AC162G_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012804.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR5076685.1"
                     /translation="MPVSTEQHLRREIIRIGKLLYDKGLIVATDGNVSARLDANRILA
                     TPSGLCKGFMEPEQLIVVDLDGNRVGSSNPANRHLKPTSEMRMHLEAYRRRPDVGAVV
                     HAHPSTTVALSIAGVDLAQCLIPEVIVTLGIVPTTQYATPSSEENARAIRDLIAGHDA
                     IVVQRHGTLTVGRDPLDAFLKTESIEQMSRIAFMARLLGASPVLPPEEVVKLIAQREA
                     LGLSRLADAEDFCAHCGVCHAPGQHVAPAERNPDEAAFVREVTERVLKRMTNAE"
     gene            1611..3053
                     /locus_tag="AC162G_12500"
     CDS             1611..3053
                     /locus_tag="AC162G_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024876.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HIR5076686.1"
                     /translation="MTKLTRHVSRITHHVSRFTLAALLAILALGASSASAQGSIVIED
                     RGVTMEFPERLTFEARIESNAEVERVVLEYGVEKLTCGTVTAKAFPDFEPAKSVDVSW
                     TWEMLQSGSEPPGATIWYRWRAFDKAGGEAVSDEERVVWLDDVHDWRSISQDMLTFHW
                     YEGTRSFAEDLLGSAVESLERLNETTGIEAESPIDMYVYAGIADMREAILYEPGWTGG
                     QARPEHDILLIGISPDNPDQVAWGRRAEAHELTHVLVGHLTFSCLGDVPTWLNEGFAV
                     YAEGRPESSWEAALEDAIAADELISVRALSGGFSEHPDRADLSYTESYSLVNFLVDEY
                     GPDKLRALFENLRDGMTIEQALNDIYGFGLDGLEDAWRVSIGAKPRRAEALKSTPYPT
                     PVPTYQPIANTALALSTPSAAPTAEAPPAAETPLHLPPQAGGVQRGAAELAAPPIGDT
                     PTATLLVVAGMIGVVVIAAVALLTRRRKSQ"
     gene            3050..5323
                     /locus_tag="AC162G_12505"
     CDS             3050..5323
                     /locus_tag="AC162G_12505"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5076687.1"
                     /translation="MRTGRLRRPRWAGGKHGRKQLVATVLALAALISACTTPTPATTP
                     ESTPLGGGQARASPPLRYEDLRQAANAPATTPTGTPTPTPTEMPTPYLPTATLPGTPE
                     AQAGRYENPDLGVSFRYPRSWVAEPGEEEASLMTLIDFPNEVIVFAGYGPMPPDATLE
                     SIAQGMRDAVSTGVSGVEYLGDEAVTLDDGREAWRSEFSMAAGDGSPVRVIIALTARS
                     GRLFTLLAFGEPDGLEQDREQIDEIIGSITLATPRLYGIPREEALFLSGGESTNPRDY
                     DPAVSGGDDLVFSGLVSFNPQLEVVPDLAESWDVSPDGTLYTFYLRENARFHDGRPVT
                     AQDVVYSWERAADPETDSDTVLTYLGDIVGVRDKHEGKVESIGGLKAIDERTLQVTID
                     APKPYFVMKLAYGTAAVVDRANVESGPEWYRTPNGSGPYKLIRWDPFELRLYERNEDF
                     YLDPPEIRYIVMPLFSGIPIRLYETGDVDIAGVGTYDAERVRDPEEPLNANLREGVSM
                     CTSFVTFDVTEPPFEDPKVRQAFALAVDKQRYIDVVLRGISLPAHGVYPPAMPGYDMS
                     YEGLPFDPELARQRLAESSYGGPENLPPIVFTSGGFGSDVDSDVAALAQMWQASLGVT
                     IEVENLEPDKFFDKLHDGEHGQLFSYGWCADYPDPENFADVLFHSDAQQNLGRYSNAE
                     LDAVLEAARVERDVGRRIGMYQQAERMIVEDAAGIFLNHGLSFVLVKPYIEGYALTPI
                     AVPIERYLSIDKEKMGE"
     gene            complement(5497..>6282)
                     /locus_tag="AC162G_12510"
     CDS             complement(5497..>6282)
                     /locus_tag="AC162G_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR5076688.1"
                     /translation="LQTSPAAVSGNLIAYNNAYLGGGVSAAGVSATLSGNLIRFNTAT
                     YRGGGVSLAGGSPMLIGNTIVSNTAPSGGGLFFILSGATLFNNVVADNRATESGSGLY
                     VGWSSPRLTHNTIARNTGGDGSGILVASFTDGSAVTLTNTILVSHTVGITVLAGDTAT
                     LQGTLWGTGAWANDMDWGGAGTILTGTINVFDDPAFVNPDTGDYHIGSTSAAINAAVN
                     AGVHTDVDNQPRPYQTPDLGADEYWPPGTLKYVYLPIVLRQLP"
BASE COUNT         1164 a         1981 c         2028 g         1109 t
ORIGIN      
        1 gggcaccgtc gtcgcgacgc gcaaatacac cggcctggaa ggcgtcaagt tcctgatcgt
       61 ccagccgctc gacaagcgcc agcagccgga cggcgatcca ctcgtcgccg ccgatgcgac
      121 ggcgcaggcc gggccgggtc acctggtgtt cgtcgtcggc gttcgcgaag cggcgctcgc
      181 cctgccggtg aagttcgtgc ccgtcgatca cgccatcgtc ggcattgtcg accaggttga
      241 tacagttcct tagagttcct tgaattccga ggggactgtg ggaactgaag gaactgaggg
      301 atttgttcac gacttgacta tgtacatcgg acgcgtcttc ggaacggtcg tcgcgaccat
      361 caagcacgag actttcgcca accgcaagct gctgctggtc gagcggctgt cgccggaagg
      421 cgagccggac aagaagtacg acgtcgccgt cgacgtggtg caggccggcg tgggcgatcg
      481 ggtgttggtg ctcgacgaag gcaactcggc gcgacagatc gtcgacctgg agccggacgg
      541 agctatccgt gcggtcgtcg tcggcatcgt ggacgagatc gatctttcct agcgcgccgt
      601 tcgccgcaag catctccccc gttctactcg ccgtcatgag gatttcccca gctgaaccca
      661 agccataact gcaggcttca agtagcagtc ctattccccc atctctccga cttcggttat
      721 acttgcatcg agcgctgagc tacattattg catcgaaagc gatccccaac tcgcatgcct
      781 gtttccaccg agcaacacct gcgtcgcgaa atcatccgca tcggcaaact gctgtatgac
      841 aagggcctga tcgtcgccac cgacggcaac gtctcggcgc ggctcgacgc gaaccgcatc
      901 ctcgccacgc ccagcgggct gtgcaagggc ttcatggagc cggagcaact gatcgtcgtc
      961 gacctggacg gcaatcgtgt tggatcatcc aaccccgcca atcgccatct caagccgacc
     1021 tcggagatgc ggatgcatct ggaagcctac cgccgccggc ccgacgtcgg cgccgtcgtg
     1081 cacgcgcatc cttcgacgac agtcgcgctg agcatcgccg gcgtcgacct ggcgcagtgc
     1141 ctgatccccg aagtgatcgt cacgctgggc atcgtcccga caacgcagta cgccacgccc
     1201 tcttcggaag aaaacgcccg cgccattcgc gacctgatcg ccggacacga tgcgatcgtt
     1261 gtgcagcggc acggcacgct gacggtcggc cgcgatccgc tcgacgcttt cctcaagacc
     1321 gaatcgatcg agcagatgag ccgcatcgcc ttcatggccc ggctgttggg cgcgtcgccc
     1381 gtcttgccgc cggaagaggt ggtaaagctc atcgcgcagc gtgaagcgct cggcctgagc
     1441 cggctggcgg atgcagagga tttctgcgcc cactgcggcg tgtgccacgc gcccggtcag
     1501 cacgtcgcgc cggcggagcg gaacccggac gaggcggcct tcgtccgtga agtgaccgag
     1561 agggtgctga agagaatgac gaatgcggaa tgacgaatga cgagtgacga atgacgaaac
     1621 tcacgcgtca cgtatcacgc atcacgcatc acgtttcacg ttttacgttg gccgcgctgc
     1681 tggcaatatt ggcacttggc gcatcgagcg cctcggcgca aggttcgatc gtcatcgagg
     1741 atcgcggcgt cacgatggag ttccccgagc gcctgacttt tgaggctcgc atagagagca
     1801 atgctgaggt cgagcgcgtg gtgctcgaat atggcgtcga gaagctcacc tgcggtacag
     1861 tcaccgccaa agccttcccc gatttcgagc cggccaagtc ggtggacgtg tcgtggacgt
     1921 gggaaatgct gcagtcggga tcggagccgc cgggcgcgac gatctggtat cgctggcgcg
     1981 ccttcgacaa ggccgggggc gaggctgtgt cggacgaaga gcgcgttgta tggctggacg
     2041 acgtccacga ttggcggagc atcagccagg acatgctcac cttccactgg tacgagggga
     2101 cgcgcagttt cgccgaggac ctgctcggct cggccgtgga gtccctggaa cgcctgaacg
     2161 agacaaccgg catcgaggcg gagtcgccga tcgacatgta tgtctacgcc ggcatcgccg
     2221 acatgcgcga agcgattctt tatgagccgg gctggaccgg cggccaggcc cgtcccgagc
     2281 acgacattct gctgatcggc atcagcccgg acaacccaga ccaggtggcg tggggcagac
     2341 gcgcggaggc gcacgagctg acgcacgtgc tcgtcgggca tctcacgttc tcgtgtctgg
     2401 gcgacgtgcc gacctggctc aacgaaggct tcgccgtgta cgccgagggc aggccggagt
     2461 cttcttggga agctgcactc gaggatgcga tcgccgccga tgagttgatc tccgttcgcg
     2521 cgcttagcgg gggcttctcc gagcatcctg acagagcgga tctgtcgtac acggagtcgt
     2581 acagcctggt caattttctg gtcgacgaat atggcccgga taagctgcgc gcgctgttcg
     2641 aaaacctgcg cgacggaatg acgatcgagc aggccctgaa cgacatctat ggattcgggc
     2701 ttgacgggct ggaggatgcc tggcgcgtat cgatcggcgc gaagccgcgc cgagccgagg
     2761 cgctgaagtc gactccgtat ccgactcccg tcccgaccta ccagccgatc gccaacacgg
     2821 cactcgcgct gtccacgccc tcggcggcgc cgaccgccga agctccgccg gcagcagaga
     2881 cccccctcca tctccccccg caggcggggg gagtgcagag gggggctgcc gagctggcgg
     2941 cgcctcccat cggggatacg ccaacagcta cgctcctggt cgtggcagga atgatcggcg
     3001 tggtcgtcat tgccgcagtc gccctcctga cgcgcaggag gaagagccaa tgaggaccgg
     3061 ccgcctccgt cgaccccgct gggcgggggg caagcacggg cgcaagcagc tggtcgcgac
     3121 agttctggcg ctggctgcgc tgatttcagc gtgcacgacg ccgacgcccg cgaccacgcc
     3181 ggagtcaacc ccgctgggcg gggggcaagc acgggcgtcc cctccgctcc gctacgagga
     3241 tctgcgtcaa gcagcgaacg cgccggccac gacgcccacc ggcacgccca cccccactcc
     3301 gaccgaaatg cccacgccct acctgccgac ggcgacgctg cccggcacgc ccgaggcgca
     3361 agccggccga tacgagaatc ccgatctggg cgtctcgttt cgttacccgc gctcgtgggt
     3421 tgccgagccg ggtgaagaag aagcctcgct gatgacgttg atcgatttcc caaacgaggt
     3481 catcgtgttc gccggctatg ggccaatgcc gccggatgct acgctcgaga gcatagccca
     3541 aggaatgcgc gatgcggtat cgaccggcgt ttccggcgtc gagtacctcg gagacgaagc
     3601 cgttacgctc gacgacgggc gcgaggcctg gcggagcgag ttctccatgg cggcgggcga
     3661 cggctcgccg gtgcgggtca tcatcgcgtt gacggcgcgc tcggggcggc tcttcacgct
     3721 gctcgcgttc ggcgagcctg acggcctcga gcaggaccgg gagcagatcg acgagatcat
     3781 cggcagcatc acgctcgcca cgccgcgcct gtacggcatt ccgcgcgaag aggcgctgtt
     3841 cctgtcgggc ggcgagtcga cgaatccgcg cgattacgat ccggccgtca gcggcggcga
     3901 tgacctggta ttcagcggcc tcgtctcctt caacccgcag ctcgaagtcg tgcccgacct
     3961 ggccgagtcg tgggacgtca gcccggacgg cacgctctac accttctact tgcgcgagaa
     4021 cgcgcgcttc cacgacgggc ggccggtcac ggcgcaggac gtggtctatt cgtgggagag
     4081 ggccgccgat cctgaaacgg attccgatac agttctgacc tacctgggcg acatcgtcgg
     4141 cgtgcgcgac aagcacgagg gcaaggtcga atcgatcggc ggcttgaagg cgatcgacga
     4201 acgcacgctg caggtcacca tcgacgcgcc gaagccgtac ttcgtgatga aactggctta
     4261 cggcaccgcc gccgtcgtcg accgggccaa cgtcgaatcg gggcccgaat ggtatcgcac
     4321 tcccaacggg agcgggcctt acaagctgat tcgctgggat ccgttcgagc ttcgtctata
     4381 tgagcgcaac gaggactttt acctcgatcc gcctgagatc cgctacatcg tcatgccgct
     4441 cttctccggc attcccattc gcctgtacga aaccggcgac gtcgacatcg ccggcgtcgg
     4501 cacgtacgac gccgagcgcg tgcgcgatcc cgaggagccg ctcaacgcca acctgcggga
     4561 gggcgtgagc atgtgcacct cgttcgtcac cttcgacgtg acggagccgc cgtttgaaga
     4621 tccgaaggtg cgccaggcct tcgccctggc cgtcgacaag cagcgctaca tcgacgtcgt
     4681 cctgcggggt atcagtctcc ctgcgcacgg cgtgtatccg ccggcgatgc cgggctacga
     4741 catgagctac gagggcctgc cgttcgaccc cgagctggcc cggcagcggc tggccgagtc
     4801 gagctacggc ggcccggaaa acctgcctcc catcgtcttc accagcggcg gcttcggcag
     4861 tgacgtggat tctgacgtgg cggcgctggc gcagatgtgg caggcgagcc tgggcgtcac
     4921 gatcgaagtg gagaatctcg agcctgacaa gttcttcgac aaactgcacg acggggagca
     4981 cgggcagtta ttctcctacg gctggtgcgc cgactatccc gacccggaga acttcgccga
     5041 tgtgctgttt cattcggacg cgcagcagaa cctgggcagg tacagcaatg cagagctgga
     5101 cgccgtgctc gaggctgccc gcgtcgagcg cgacgtgggc aggcgcatcg ggatgtacca
     5161 gcaggccgag cggatgatcg tcgaggacgc cgccggcatc ttcttgaatc acgggctgag
     5221 cttcgtgctg gtcaagccgt acatcgaggg ctacgcgctg acgccgatag ccgtgccgat
     5281 cgagcggtat ctgtcgattg ataaggagaa gatgggggaa tgaggagtga tcagtaatca
     5341 gtgataagta attggtaatt agtgatgcgc aatgtgcaac tctttccagc atgcctatat
     5401 gggtttcatg caacaacaac ccaaggcgga gcaataaccc gtcaaggacg taacaacaaa
     5461 cctcccgatg gatttaagcc ttcgggaggt tctgtgtcat ggaagctgcc gaagcacaat
     5521 aggcaggtag acgtatttca gtgtgccggg cggccagtat tcgtcggcgc ccaggtctgg
     5581 ggtctgatag gggcgcggct ggttgtcaac gtctgtatgg acacccgcat tcacggctgc
     5641 attgattgcg gcactcgtcg agccgatgtg gtagtccccc gtgtcaggat tgacaaacgc
     5701 cggatcatcg aatacattga tcgtgccggt gagaatggta cctgcgccgc cccaatccat
     5761 gtcgttagcc caagcgccag tgccccatag cgtgccttgc agtgtagccg tgtcgccggc
     5821 cagcacagta atgcccaccg tgtggctaac caatatcgtg ttggtcaagg ttactgcgct
     5881 gccatcggta aacgaggcga caagaatacc gctgccatct ccacccgtgt tgcgagcgat
     5941 cgtgttgtgt gtcaggcgtg gggatgacca cccgacatac agtccgctgc cgctctcggt
     6001 ggcgcgattg tcggccacga cattattgaa cagcgtggca ccgctcagga tgaagaacaa
     6061 tccgcctccg gacggggcag tattcgagac gatcgtgttc ccgatgagca tgggagagcc
     6121 gccggcaagg ctcacgccac cacctcgata tgtggcggta ttgaagcgga tgaggttgcc
     6181 gctgagtgtg gcgctgacgc cggcggctga cacgccgcca cccaggtacg cgttgttgta
     6241 ggcgatcaaa ttgccactga cagcagcagg actcgtttgc ag
//
LOCUS       DBNHXB010000311         4808 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4278767, whole genome shotgun sequence.
ACCESSION   DBNHXB010000311 DBNHXB010000000
VERSION     DBNHXB010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4278767"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /gene="gap"
                     /locus_tag="AC162G_12515"
     CDS             complement(<1..978)
                     /gene="gap"
                     /locus_tag="AC162G_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR5076689.1"
                     /translation="MTIKIGINGFGRIGRQVTKALFELYKDDFDLVLVNDLTDSRTNA
                     HLFKYDSTYGRFDGTVEYDESSLTINGDKIKVMAEKDPARLPWKDLGVDIVVESTGVF
                     TDATKARAHLDAGAKKVIISAPAKNEDITIVLGVNDDKYDPSKHHIISNASCTTNCLA
                     PAAKVVHDSFKIVKGLMTTVHAYTNDQRILDLVHKDLRRARAAGQNIIPTTTGAAKAV
                     ALVIPDLKGKFDGFALRVPVPTVSIVDFVCNVEKVPASAADVNAAFKAAAEGPMKGIL
                     GYTEEPLVSSDFRGDTRSSIVDGLSTMVKDDLVKVIAWYDNEWGYSVRTA"
     gene            complement(1021..2217)
                     /locus_tag="AC162G_12520"
     CDS             complement(1021..2217)
                     /locus_tag="AC162G_12520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIR5076690.1"
                     /translation="MSLTRRRFLTMLGIAGAATALSLRPDPSAAESPIQDLLPYYLES
                     ARRNVVTRRGERAILSSRIYPKGTFVRDAFYGPMALGDVKLSHDCYRWFERTQLPNGQ
                     IRTAVGFTPKDQAVLAPMDDDSSLLFIIWSAWLGHRGIEIDRETVERAFAHVRSHVDE
                     RGEFVSPPGAFRYWADTVSPEAPERITHNQGLYALALRSMYALGWGDVTLAHVTAAKR
                     RYAACYDTAAGSLTLARDTWWAPKLDISTVFPEFLFRWAFGDGALPNSIVTATVARHV
                     RNASVYKDGKIAGIKVICDADGSFLPAARFHVPVLNSQGDYQNGGYWPMYTLTALALA
                     YKIAPSRERRSIVETLVAAELSSDHESKEFIVLQPGAIGASNPRRNGYTWNALIAPAL
                     EWAGIV"
     gene            complement(2252..3628)
                     /locus_tag="AC162G_12525"
     CDS             complement(2252..3628)
                     /locus_tag="AC162G_12525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HIR5076691.1"
                     /translation="MRTPLRNYLLDRLRASGLAHWLTIGIGVKRWLLVLLAGVTALGL
                     GLAYFLVDILPGQFLPDAINLLTLQFIPIPVRALLFSVAGVILIAIAIVKRSRSLLVP
                     FTPTGAVPALQAMHQHPQREREPKIVAIGGGTGLSTLLRGLKAYTSNITAIVTVADDG
                     GSSGRLRREMGILPPGDFRQCIAALADDEALTTRLFRYRFSARPSGGNGSGLSGHAFG
                     NLFIAAMASVSGSFERGLVESSRVLAVRGRVLPSTVTPLTLVGDLRDDATNSLRRVEG
                     ESVITHAGATIVRLAIKPDDVRAYPDAIRAILDADVIILAPGSLYTSLLPNLLVPGIS
                     DAIRAARGMRLYICNVATQRGETAHYAVRDHVLAIERHVGAGLIDAILANSNTEIAWA
                     NTPPGVSEMIRFEPGAPPADPPIFGRDMIDTAAPWRHDSQKVAQAVMQTLSELRPDEF
                     CAPARMRL"
     gene            3819..>4808
                     /locus_tag="AC162G_12530"
     CDS             3819..>4808
                     /locus_tag="AC162G_12530"
                     /inference="COORDINATES: protein motif:HMM:NF013811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR5076692.1"
                     /translation="MADFDLLKELIVPSTSKIVLLLMDGLGGIPREPNGPTELEHART
                     PHLDRLASEGSLGLIVPVRPGITPGSGPAHLALFGYDPVTHQIGRGVLESLGIDFDLQ
                     PGDVAARGNFCTVDDEGRIVDRRAGRIPTEKSVALCDKLRAITLPGVEVFVEPVKDYR
                     FCLILRGEGLDSRIEDTDPQRQAPPLPARAIVPEAEPTAALINEWIGKAREILKDERP
                     ANMLTLRGVASDPGLPRFPDLYGLRSACVAVYPMYRGVSRLVGMDVIEFDGESPADEF
                     ETVKSFWDTYDFFFVHVKPTDSRGEDGDFEGKAKVIESVDAALPALLALKPDVL"
BASE COUNT          843 a         1495 c         1595 g          875 t
ORIGIN      
        1 agccgtgcgc accgagtagc cccactcgtt atcgtaccag gcgatcacct tgaccaaatc
       61 gtctttcacc atcgtcgaca agccgtcgac gatcgacgag cgggtgtcgc ccctgaagtc
      121 gctggaaact agcggctctt ccgtgtagcc caggatgcct ttcatcggcc cttcggcggc
      181 ggctttgaac gcggcattca cgtcggccgc cgaagccggc accttctcga cgttgcacac
      241 gaaatccacg atcgagacgg tagggaccgg cacacgcagc gcgaagccgt cgaacttgcc
      301 ctttagatcg gggatcacca gcgccacggc cttcgccgcg ccggtcgtcg tcgggatgat
      361 gttctgcccg gcggcgcgcg cgcggcgcag gtccttgtgc accaggtcga gaatgcgctg
      421 atcgttcgtg taggcgtgca cggtcgtcat caagcctttg acgatcttga agctgtcgtg
      481 aacgaccttg gccgccggcg cgagacagtt ggtcgtgcag gaggcgttcg agatgatgtg
      541 gtgtttcgag ggatcgtact tgtcgtcgtt gacgcccagg acgatggtga tgtcctcatt
      601 cttggctggc gcgctgatga tgactttctt cgcgcccgcg tcgaggtgcg ccctggcttt
      661 cgtcgcgtcg gtgaatacgc cggtcgactc gacgacgatg tccacgccca ggtctttcca
      721 cggcagcctg gccgggtctt tctcggccat gaccttgatc ttgtcgccgt tgatcgtcag
      781 gctgctctcg tcgtattcga ccgtgccgtc gaagcgaccg taggtcgagt cgtacttgaa
      841 cagatgcgcg ttggtcctgg aatcggtcag gtcgttgacg aggacgaggt cgaagtcgtc
      901 cttatacagc tcgaacagcg ctttcgtgac ctgccggccg atacgtccga acccgttgat
      961 gccgatcttg atagtcatgt ctgcttgctc ctttgaagag gtggtgaaat tgacaacatg
     1021 tcacacgata cccgcccatt ccagggccgg cgcgatcagc gcattccacg tgtagccatt
     1081 ccgacgcggg ttcgacgcgc cgatcgcgcc aggctgcaag acgatgaact ctttcgactc
     1141 gtggtctgaa gacagctcgg ccgccacgag cgtctcgacg atcgaccggc gctcacgcga
     1201 cggcgcgatc ttgtaggcca gcgccagcgc cgtcagcgta tacatgggcc aatagccgcc
     1261 attctggtag tcgccctggc tgttcagcac ggggacgtga aaccttgcgg cgggcaaaaa
     1321 agacccgtct gcgtcgcaga tgactttgat gcctgctatt ttaccatctt tgtataccga
     1381 tgcattgcgc acgtgccgcg cgacggtcgc ggtgacgatc gagttgggca gcgcgccgtc
     1441 gccgaatgcc cagcggaaca ggaactccgg aaacacagtc gagatatcca gcttgggcgc
     1501 ccaccacgta tcgcgcgcca gcgtgagcga tccggcggct gtgtcgtaac atgctgcgta
     1561 tcgtctcttg gccgctgtga cgtgcgccag agtgacgtcc ccccagccca aggcgtacat
     1621 ggatcgtagc gccagggcat acagcccctg gttgtgcgtg atgcgctccg gcgcctccgg
     1681 gctgaccgtg tccgcccagt agcggaacgc ccccggcggc gacacgaact cgccacgctc
     1741 gtcgacatgc gatcgcacgt gcgcgaaggc gcgctcgacg gtttctcgat cgatctcgat
     1801 gcccctgtga ccgagccagg ccgaccagat gatgaacaga agggaagaat cgtcgtccat
     1861 cggggcgagg acggcctgat cctttggcgt gaatccgacc gccgtgcgga tctgcccgtt
     1921 cggcagttgc gtccgctcga accagcgata acagtcgtgg cttagcttca cgtcgcccag
     1981 cgccatcggg ccatagaacg cgtcgcggac gaaagtgcct ttcgggtaga tgcggctgga
     2041 taggatcgcg cgctcgcctc ggcgggtgac gacattccgg cgggcgcttt ccagataata
     2101 cggcagaagg tcctgaatag gagactcggc cgcgctcgga tcgggccgaa gagagagcgc
     2161 cgtcgcggcg ccggcgatgc ccagcatcgt caggaatcgg cgtcgagtaa gagacatgct
     2221 cgtcgggacg cgaccggtcg cggatcgccg gtcacagtct catgcgtgcc ggcgcgcaga
     2281 attcgtccgg gcgaagctcg ctcagcgtct gcatgacggc ctgagctacc ttttgactgt
     2341 cgtgacgcca cggcgcggcg gtgtcgatca tgtcgcggcc gaagatgggc ggatcggcag
     2401 gcggcgcgcc cggttcaaac cggatcatct cgctgacgcc gggcggcgtg ttggcccagg
     2461 ctatctccgt attcgagttg gcgaggatgg cgtcgatcag ccctgcgccg acgtggcgct
     2521 cgatggccag gacgtgatcg cgcaccgcat agtgagccgt ttcgccgcgc tgggtggcga
     2581 cgttgcagat gtacaggcgc atcccgcgcg cggcgcgaat cgcgtcggag atgcccggca
     2641 ccaggaggtt gggcagcagg ctggtgtaca ggctgccggg cgcgaggatg atcacatcgg
     2701 cgtcgaggat ggcgcggatg gcgtcggggt aggcgcgcac gtcgtcgggc ttgatcgcca
     2761 gccggacgat cgtcgcgcct gcgtgcgtga tgaccgactc gccctcgacg cgcctgagcg
     2821 agttggtcgc atcgtcgcgc agatcgccga ccagcgtcag cggcgtcacc gtggacggca
     2881 gcacgcgccc gcgcacggcc agcacgcggc tcgactcgac gagtcctcgc tcaaacgagc
     2941 cgctgacgct cgccatcgcc gcgatgaaca gattgccgaa cgcgtgcccg ctcagccccg
     3001 atccgtttcc gccggacggg cgcgcgctga aacgataccg gaagagacgc gtggtcagcg
     3061 cttcgtcgtc ggcgagggcc gcgatgcact ggcggaagtc gccgggcggc aggatgccca
     3121 tctcgcgccg cagccgtccg gacgatccgc cgtcgtcggc gacggtgacg atggcggtga
     3181 tattcgaagt atacgccttc aggccgcgca gcagcgtcga gaggccggtg ccgccgccga
     3241 tcgcgacgat cttcggttcc cgctcccgct gggggtgctg gtgcatcgcc tgcagcgccg
     3301 gaacagcgcc cgtcggagtg aacgggacga gcagcgaccg gctcctcttg acgatagcga
     3361 tggcgatgag tatgacgccg gcgacgctga acagcagcgc ccgcaccgga atcgggatga
     3421 actggagggt caggagattg atggcgtcgg gcaggaattg gccggggagg atgtcgacca
     3481 ggaagtacgc cagccccagt ccgagagctg taacgccggc cagcaggacg agcagccacc
     3541 gcttgacgcc aatgccgatg gtcaaccagt gggcaagtcc gctggcgcgc aggcgatcga
     3601 gcaggtagtt acgtaggggc gtgcgcacgg taaaatcgat tctatcagaa tcgatctgag
     3661 gtgtcaaaac ctttagggtt atcggcgtta gacgcgcaag cgttgagtga tataatcgtg
     3721 ccaatcctta tttcgacgat tcccccgctg gatctggcaa tgcagcgtga gcattcactc
     3781 ggaattctgc aatgcgctga gcatgagaaa ggagccgtat ggccgacttt gacttgctga
     3841 aagagctgat tgtgcccagc acgtccaaga ttgttctgct tctcatggac ggcctgggag
     3901 gcattccccg cgagccgaat ggcccgacgg agcttgagca tgcccgcact ccccatttag
     3961 acaggctggc gtcggagggc agcctggggc tgatcgtccc ggtgcggcca gggatcaccc
     4021 ccggatcggg accggcgcac ctggcgctgt tcggctacga cccggtcacg caccagatcg
     4081 gccgcggtgt gctcgaatcg ctgggcatcg acttcgacct gcagccgggc gacgtggcgg
     4141 cgcgcggcaa tttctgcacg gtcgacgacg aaggccggat tgtcgatcga cgggccggac
     4201 gaattccgac ggagaagagc gtcgcgctgt gcgacaagct gcgcgcgatc acgctgcccg
     4261 gcgtcgaggt gtttgtcgag ccggtgaaag attatcggtt ctgcctgatc ttgcgcggcg
     4321 aagggctgga cagccgcatc gaggataccg atcctcaacg ccaggccccg ccgctgcccg
     4381 cgcgcgcaat cgttcccgaa gcggaaccca cggctgcgct gatcaacgaa tggatcggca
     4441 aggcgcgcga gatcctcaag gacgagcgcc ccgccaacat gctcaccctg cgcggcgtcg
     4501 catcggatcc cggcttgccg cgcttccccg acctgtatgg cttgcgctcg gcctgcgtcg
     4561 ccgtgtatcc gatgtacagg ggagtgtcgc ggctggtggg catggacgtc atcgagttcg
     4621 acggcgagtc gcccgccgac gagttcgaaa ccgtgaaatc tttctgggat acctatgact
     4681 tcttcttcgt ccacgtcaag ccaacggaca gccgcggcga ggatggagac ttcgagggca
     4741 aggcgaaagt gatcgaatcg gtcgacgccg cgctgcccgc gctgttggcg ctcaagcccg
     4801 acgtcctg
//
LOCUS       DBNHXB010000312        10288 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3607728, whole genome shotgun sequence.
ACCESSION   DBNHXB010000312 DBNHXB010000000
VERSION     DBNHXB010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10288
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3607728"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..1709
                     /locus_tag="AC162G_12535"
     CDS             297..1709
                     /locus_tag="AC162G_12535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5076693.1"
                     /translation="MSIRLRLTLWYTLVLSAILAAVGFAIYSVFAATLLEDADRQLDE
                     TAAQVRAVSRAFNVLGEIRINVPVEADVFRAGGLHLQVIDASGRVIRVSDSLRGYNQP
                     INPAVLSLDLSDLRPVRHDVHTGDVHLRVLTVPIGSPEQIIGYLQIGLQMDSYDESLS
                     QLTRLLLGGWMVGGLLGGVGGAFLARRALRPIDDVTSTARAIAEAGDLGRRIPVSRAR
                     DEVGRLAMTFNDMLTRLEALFKAQQRFTADISHELRTPLTTIRGNLDLIRRMRVADDA
                     SLDAAQGEIERMTRLVGDLLLLAQADAGLPIRREPVALDAVMYEVFDQMRTIADGVSV
                     GASAEDAVAVMGDADRLKQLMINLVGNAVRYTPAGGSVTLNLKRRDGWAVFSVSDTGL
                     GIPPEHLPHIFERFYRVEKSRSHHEAGVNGASSGAGLGLSIAQWIAQSHGGRIEAESE
                     VGKGTVFTVYLPELRVIRET"
     gene            1836..3881
                     /locus_tag="AC162G_12540"
     CDS             1836..3881
                     /locus_tag="AC162G_12540"
                     /inference="COORDINATES: protein motif:HMM:NF044618.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076694.1"
                     /translation="MIRHFAVLFVAFAVALAACTPPSPTPTAVPAASPTPSEQPVRTE
                     APPDTPSPEPPTAGQTPEATLGPLDGILREPVTDADRANAAAIGAADLPIGDLRELAI
                     RFKGLPADTPEKTCTTAPAHNLGDRAVFQVSNNDTFEQFEVTATLVSKEPNVYMWVDN
                     KWLGQIDRNAVAAAAKELSQKIIPRNRELFGLEWSPGIDCDENLHVLHTSNTAAGGYF
                     SSVDQYTTAVREDSNEKEMFYVDVEGIGAPSLIGSSFYLGTLAHEYQHMIHFHVDRNE
                     DTWANEGLSDLAMFLNGYSVGGADFAFAEEPNTQLNFWPEGGGRGENYGASYMFWLYF
                     YDKYGEQGITEIVADPRNGLDGVAGSLQRVGYPGVLDDFFADWVIAKFVDSPSMGDGR
                     YGFEETDPPTADIAQTFDSFPHQFMRPRRVNQYAAQYYQLRGDRDLHVDFVGSTRARM
                     IDAEPHSGETFWWSNRGDSADMRLTREVDLTGVDKATLSYWTWHDIEADWDYAYVSVS
                     TDGGQTYQTLKTPSGTDRNPNNNNLGWGYTGNSGGGEDPVWIQESVDLTPYVGKKILL
                     RFEVVNDLAVNLPGFAIDDVEIPEISFQDDAETDTGWTAEGWIRTNNFVPQNFIAQVI
                     GYSKDGSTTVTRLPINPDNTGAWDIPLGQWNSGVLALAATAVKSSEPALFNWTITEK"
     gene            complement(3993..4211)
                     /locus_tag="AC162G_12545"
     CDS             complement(3993..4211)
                     /locus_tag="AC162G_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIR5076695.1"
                     /translation="MNKFMAFAAGALCGALAGAVVALLLTPMSGTEMQEQARTRFDEI
                     VEEGRKAAEQRRAELERQLRQLQGKEPA"
     gene            4295..5404
                     /locus_tag="AC162G_12550"
     CDS             4295..5404
                     /locus_tag="AC162G_12550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR5076696.1"
                     /translation="MSLQRIARLQDAARAEGFDCIAVMPGANMVYLTGLHFHLMERPT
                     LALIPAEGSAPALVLGALEATKPAAGPHPIDWQLFTFADGTDPALAYNAAGQALNLAG
                     RRIGVETLAMRVKEMRLLGAAAPGARLDEADHIFARLRRVKDADELAAMRHAAHIAED
                     ALRRTLEAVQVGMTEKQIAAELLVNLLKGGAENLPFEPIIVAGPNSALPHATASDRAV
                     QRGDLLLFDFGATVEGYHSDITRTFAAGKIDDELKRVYDLVLKANEAGRATAEPGVSG
                     EAVDRAARKVIVDGGYGAYFTHRTGHGLGMDAHESPYVVEGNTTPFEPGNVITIEPGI
                     YLEGKGGVRIEDDVVITASGAESLTTFPRELITIG"
     gene            complement(5444..5668)
                     /locus_tag="AC162G_12555"
     CDS             complement(5444..5668)
                     /locus_tag="AC162G_12555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR5076697.1"
                     /translation="MPIYEYFCADCRAKFEVLRPMAQADEKIACETCGGGHTARVLSV
                     FAIKGGDGASVMESHGGGCACGGACACGGH"
     gene            complement(5716..6906)
                     /locus_tag="AC162G_12560"
     CDS             complement(5716..6906)
                     /locus_tag="AC162G_12560"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5076698.1"
                     /translation="MDLFQKARESELMQIARESIESGIYPYFKPLQDTEGTEVIMDGR
                     RVLMIGSNNYLGLTTHPKVREAAIDAIHRYGTSCTGSRFLNGTLALHHELEHKLAQFV
                     GKEAALVFSTGMQVNLGTISAIVQRGDVVVIDKDDHASIVDGCRLSYGQMKRFPHNDV
                     EALERVLKSIPDDRGVLVVVDGVFSMGGDIAPLPQLVEVCKTYGARLMVDDAHACGVL
                     GRNGRGTADHFGMTEHVDLIMSTFSKSFASLGGFVAGDDDVIFYIKHHARALIYSASM
                     PPSNVAAVMAALEVMLEEPERVERVNAIGDRVRRELKGMGYDIGPSQTPIVPVIIGDR
                     DRTVFAWNMFYQAGLYTNCVLSPGVPPGMDLLRTSYMATHTDEQIDRVLEVFYTVGKE
                     LELI"
     gene            7038..7715
                     /locus_tag="AC162G_12565"
     CDS             7038..7715
                     /locus_tag="AC162G_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HIR5076699.1"
                     /translation="MCGRFTLTVDPSELQQAFPWLKLPADPSLTPRYNVAPTQGVAGV
                     ANNSQNTLEMFRWGLIPAWSKDAAIGNRLINARAETLADKPSFRLAFKRRRCLILADG
                     FYEWRKEPNGKTKTPMYIRLKSGEPFAFAGLWEAWRSPEDKSILTCTIITTEPNALLA
                     KIHDRMPVILKPEAYMRWLDPEPREPEEMSKLLKPYPASQMTAYAVSRAVNNPANDSP
                     ECIVPMK"
     gene            7788..8054
                     /locus_tag="AC162G_12570"
     CDS             7788..8054
                     /locus_tag="AC162G_12570"
                     /inference="COORDINATES: protein motif:HMM:NF051948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076700.1"
                     /translation="MSQDAIQRAMEVKRRHEKELMRRPNVVGVGVGFRTRGGVRTDEA
                     CIVVSVKVKLAASQMKPGDLIPPSIEGVPVDVVETGEITALGGR"
     gene            8086..9075
                     /locus_tag="AC162G_12575"
     CDS             8086..9075
                     /locus_tag="AC162G_12575"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chymotrypsin family serine protease"
                     /protein_id="HIR5076701.1"
                     /translation="MDAMQSEALAAARQVKRESVVTLMKMANVVATGVGYKISGDTQT
                     GELAVVVSVRKKLPLAQLAESDIVPKTIDSVKTDVIETGEIVAFQDPRERMRPARPGV
                     SIGHFEITAGTLGCLVRKNGEVHILSNNHVLANSNNAQIGDAILQPGPADGGTSVDQI
                     GTLAEFIPIAFDGTLPPPGGCLTQLLSAVGLQPRAPMQNEPGNNKVDCALARPTTPDL
                     VDPNILNIGVPIGAGTATLGTPVQKSGRTTGYTTGTILQIDVTVTVNYGGPFATFTGQ
                     LMAGAISQGGDSGSAVLDMNKRVVGLLFAGSPTTTIINPIQFVLDMLGVTIVT"
     gene            complement(9195..9755)
                     /locus_tag="AC162G_12580"
     CDS             complement(9195..9755)
                     /locus_tag="AC162G_12580"
                     /inference="COORDINATES: protein motif:HMM:NF021386.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HIR5076702.1"
                     /translation="MTMEENALNELLAFFKALVDPDRLAIAGRLARSSATVETLATEL
                     DMPRIDVQRHLDRLVEAGLVAASGGVYTLDHHALHAQAKRVLSSGAPASPPATSLEKV
                     LADYLRPDGSLKEIPSQLKKRMIVYEHIASHFEIGRHYTEKEVNELLRRFHPDVVSMR
                     RDLFDLGLINRRQDGSDYWRSESNTF"
     gene            9850..>10288
                     /locus_tag="AC162G_12585"
     CDS             9850..>10288
                     /locus_tag="AC162G_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HIR5076703.1"
                     /translation="MSKRQVHTVKIAGLTRDLPLFEVAPGVHIAIFNMLGDTEIVLAA
                     AKALARRLPKDAQTLVTAEAKSIPLAHALSVESGLPYVVLRKSYKPYMGVAVSAETLS
                     ITTGQPQTLYLDEKDRALVDGRKIVLVDDVISTGSTLQGMRLIL"
BASE COUNT         1932 a         3169 c         3364 g         1823 t
ORIGIN      
        1 gagaagaatg acggatgtag cgtgtcgaat gaagaatgca gaatgaagaa aaaagcggcg
       61 tctatcatcc cgacttaatt ctgccctcat ccgtctctca tttgcgcggc ctagacttat
      121 caataggagt ctggtgaatt tccggagtcg agcctttagg cggtcatgtc tgacgatcga
      181 atccggctaa aacctcgact ccagcgccaa atgaggcggt cgccaaactc cagttctcaa
      241 gggactgtcc ttggagcgac ccaatttcta cattctacat tctgcattga ccttcgatgt
      301 ctatccgcct ccgcctgacc ctgtggtaca cgctcgtctt gtccgcgatc ctcgcggcgg
      361 tcggttttgc catctacagc gtcttcgccg ccacgctcct ggaggacgcg gaccgccagc
      421 tcgacgagac ggcggcgcag gtacgggcgg tcagccgcgc gttcaacgtg ctcggcgaaa
      481 ttcggatcaa cgtgccggtg gaagccgacg tctttcgtgc cggcgggttg cacctgcaag
      541 tgattgacgc gtcgggccgc gtgatccgcg tctccgactc gctgcgcgga tacaaccagc
      601 cgatcaaccc tgccgtgctc agcctcgacc tgtcggactt gcgccccgtg cggcacgacg
      661 tgcacacggg tgatgtgcac ctgcgcgtgc tgaccgtgcc gatcggctcg ccggagcaga
      721 tcatcggcta tctccagatc ggcttgcaga tggattcgta cgacgagtcg ctgtcgcagc
      781 tcacgcgtct cctgctgggc gggtggatgg tgggcgggct gctgggcggc gtgggcggcg
      841 cgttcctggc gcggcgggcg ctgcgcccta tcgacgacgt gacgagcacg gcgcgggcca
      901 tcgcggaggc tggcgatctg ggtcgccgca tccccgtttc gcgcgcgcgc gacgaggtgg
      961 gccgcctggc catgaccttc aacgacatgc tcacgcggct agaggcgctg ttcaaggcgc
     1021 agcagcgctt cacagcggac atctcgcacg agctgcgcac gccgctgacg acgattcgcg
     1081 gcaatctcga cctgatccgc cgcatgcgcg tcgccgacga cgcgtcgctc gacgcggcgc
     1141 agggcgagat cgagcgcatg acgcggctcg tcggcgacct cctgctgttg gcgcaggccg
     1201 acgcgggact gccgatccgg cgcgagcctg tggcgctcga cgcggtcatg tacgaagtct
     1261 tcgatcaaat gcgaacgatc gccgacggcg taagcgtggg agccagcgcc gaggatgccg
     1321 tcgcggtgat gggcgacgcc gaccggctca agcagctcat gatcaacctg gtcggcaacg
     1381 ccgtccgata cacgcccgct ggcgggagcg tcacgctgaa tttgaagcgg cgagacggct
     1441 gggcggtatt cagcgtgtcc gacaccgggc tgggcatccc gcccgagcat ctgccgcaca
     1501 tcttcgagcg gttctaccgc gtcgaaaaat cgcgctcgca ccacgaagcg ggcgtgaacg
     1561 gcgcgtcgag tggcgccggg ctagggctgt cgatcgcgca gtggatcgcc cagagccacg
     1621 gcgggcgcat cgaggcggag agcgaggtag ggaaggggac agtgttcacg gtgtatttgc
     1681 cggagctgag agtgatacgt gaaacgtgat agggttgctc ccgccggatt gtcaaatccg
     1741 gtagccgaaa gtggcggata tgactatccg ccgcgagcag cagaatgtgc gttgcacccg
     1801 tttgagacat gagccaaggt cgaggagcgt cataaatgat tcgccatttt gcagtgctgt
     1861 tcgtcgcgtt cgccgtggcg ctggctgcgt gtacgccgcc tagcccgacg ccgaccgccg
     1921 tgccggccgc ctccccaact ccaagtgagc agccggtccg tacagaagcg ccacctgata
     1981 cgccgtcgcc cgagccgccg acggccgggc agacgccgga ggcgacgctc ggcccgctgg
     2041 acggaatcct gcgcgagccg gtgactgacg ccgatcgggc caatgcggcg gcgatcggcg
     2101 ctgccgatct gcctataggc gatttgcgag agctggcgat acggttcaag ggattgccgg
     2161 ccgacacgcc tgaaaagacc tgcacgaccg cgccggcgca caacctcggc gaccgagccg
     2221 tgttccaggt gtccaacaac gacacgttcg agcagttcga ggtgaccgcg acgttggtgt
     2281 cgaaagagcc gaatgtgtac atgtgggtag acaacaaatg gctcggccag atcgacagaa
     2341 acgccgttgc cgccgctgca aaggagttga gccagaagat catcccgcgc aatcgcgagc
     2401 tgttcggtct ggaatggtcg ccgggcattg actgcgacga gaacctgcac gtgctgcaca
     2461 cctccaacac ggcggcgggc ggctacttct ccagcgtcga tcaatacacg acggccgtcc
     2521 gggaagactc gaacgaaaaa gagatgttct acgtcgacgt cgagggcatc ggcgcgccat
     2581 cgctcatcgg aagcagcttt taccttggca cgctggcgca cgaataccag cacatgattc
     2641 acttccacgt ggatcggaac gaggatacgt gggcgaatga agggctgtcc gacctggcga
     2701 tgttcctgaa tggctacagt gttggcggcg ccgacttcgc ttttgcggag gagccgaaca
     2761 cccagttgaa tttctggcct gagggcggcg ggcgaggcga gaactacggc gcgtcctaca
     2821 tgttctggct gtacttctac gataagtacg gcgagcaagg tattaccgag atcgtcgccg
     2881 atccgcgcaa cgggcttgac ggcgtggctg ggtcgctgca gagagtcggc tatccgggtg
     2941 tgctcgacga tttcttcgcc gattgggtga ttgcgaagtt cgtggacagt ccgtcaatgg
     3001 gagatggacg gtatgggttt gaggaaaccg atccgccgac ggccgatatt gcccagacgt
     3061 tcgattcatt cccccaccaa ttcatgcgcc cgcgtcgcgt caatcaatat gcagcgcagt
     3121 attaccagtt acgtggcgac cgcgacctgc acgtcgattt cgtcggatcg acccgggcgc
     3181 gcatgatcga cgccgagccg cacagtggcg agaccttctg gtggagcaat cgcggcgaca
     3241 gcgcggatat gcgcctgacc cgcgaagtcg acctgaccgg cgtcgacaag gcaacgctga
     3301 gctactggac gtggcacgac atcgaagccg actgggacta cgcttacgtg agcgtgtcga
     3361 cggatggggg ccagacctac cagactctca agacaccttc cggcacggat cgcaatccga
     3421 ataacaataa tctcggctgg ggctataccg ggaactccgg cggtggcgaa gaccccgtct
     3481 ggattcagga aagcgtcgat ctcacaccat acgttggcaa gaagatcctg ctgcgcttcg
     3541 aggtcgtcaa cgacctcgcc gtgaacctgc ccggcttcgc catcgacgac gtcgagatcc
     3601 cggaaatcag cttccaggat gacgcggaga cggatacagg ttggactgcg gagggctgga
     3661 tccggacgaa caacttcgtg ccgcagaatt tcatcgcaca ggtcatcggc tacagcaaag
     3721 acggcagcac gacggtcacg cgcctgccga tcaacccgga caacaccggc gcgtgggata
     3781 tcccgctcgg ccagtggaac tcaggcgtgc tcgcgctcgc ggcgacggcg gtgaaaagca
     3841 gtgagccagc gctgttcaac tggacgatca cggagaagta gaccatttgc tcccgccggg
     3901 tgtgtcaccc ggcggctcgg ggtggggggt cgcagacccc cgacaagcgg taaagagacc
     3961 tgccaggttt cagaaacctg gcaggtcttt actcatgccg gctctttgcc ctgcaactgg
     4021 cgcagttgcc gctccagctc ggcgcgccgc tgctcggcag ccttccggcc ttcctccacg
     4081 atctcatcga agcgcgtgcg cgcctgctcc tgcatctcgg tgccggacat cggcgtcaac
     4141 agcagcgcca cgaccgcgcc ggcaagcgcg ccgcacaggg cgccggcggc gaatgccatg
     4201 aacttgttca tcgggtgcct ccgtgaatgg attgggcggg attatacact aggatcatcg
     4261 cgtgctataa ttgttgcact cgacggagac gctcatgtcc cttcaacgca tcgcccgcct
     4321 gcaagacgca gcgcgcgccg aaggcttcga ctgcatcgcg gtgatgcccg gcgcgaacat
     4381 ggtttatttg accggcctgc attttcacct gatggagcgg cccacactcg ccctgattcc
     4441 ggccgaaggc agcgcgcctg cgctggtgct gggcgcgctc gaagcgacga agccggccgc
     4501 tggcccgcac ccgatcgact ggcagttgtt cacgttcgcg gacgggacgg atccggcgct
     4561 cgcctacaac gcggcaggcc aagcgctgaa cctggccggc aggcgtatcg gcgtcgagac
     4621 gctggcgatg cgtgtgaagg agatgcgcct gctcggagcg gccgcgccgg gcgcgcgcct
     4681 cgacgaggcc gaccacatct tcgcccgcct gcgcagggtg aaggacgccg acgagttggc
     4741 ggcgatgcgg cacgcggcgc acattgcaga agacgcgctg cgccggacgc tcgaggcggt
     4801 tcaggtaggc atgaccgaga agcagatcgc ggccgagctt cttgtcaacc tgctcaaagg
     4861 cggcgcggag aacctgccct tcgagccgat catcgtcgcc ggaccgaact ccgcgttgcc
     4921 gcacgccacg gcgagcgatc gcgccgtgca gcgcggcgat ctgcttctgt tcgatttcgg
     4981 cgcgaccgtg gaaggctatc acagcgacat cacgcgcacg ttcgccgccg gcaagatcga
     5041 cgacgagctg aagcgcgtct acgacttagt gctgaaggcc aacgaggcag gtcgcgcgac
     5101 cgccgaaccg ggcgtgtcgg gcgaagccgt cgatcgagcc gcgcgcaagg tgattgtcga
     5161 cggcggctat ggcgcgtact tcacgcatcg caccgggcac ggactgggga tggatgctca
     5221 cgagtcgccg tacgtcgtcg agggcaacac gacgccgttc gagccgggca acgtcatcac
     5281 gatcgagccg ggcatctatc tggaagggaa ggggggtgtc cgcatcgagg acgacgtggt
     5341 gattacggcc agcggcgcgg agagcttgac gacgttcccc cgcgagctga taacgattgg
     5401 atgaaaagac ctgccaggtc tcggagacct ggcaggtctg cggtcaatgc ccgccgcagg
     5461 cgcacgcgcc gccacaggca cagcctccgc cgtgtgattc catcacggac gcgccgtcgc
     5521 cacctttgat ggcgaatacc gacaggacgc gcgcggtgtg gccgccaccg caagtctcgc
     5581 aggcgatttt ttcgtcggcc tgggccatcg ggcgcaacac ctcgaatttg gcccggcaat
     5641 cggcgcagaa atattcgtaa atcggcatcg ttcctccccg gctgggtgtt gcgtttcagg
     5701 ataatccggc tatacttaaa tcagttccag ctccttgccc accgtgtaga agacttccag
     5761 gacgcgatcg atttgctcgt cggtgtgggt cgccatgtac gatgtgcgca gcaggtccat
     5821 gcccggcggc acgcccggcg agagcacgca gttggtgtac aggccggcct ggtagaacat
     5881 gttccaggcg aacacggtcc ggtcacggtc gccgataatg accggcacga taggcgtttg
     5941 gctggggccg atgtcgtagc ccatgccttt cagttcccgg cgcacgcgat cgccgatcgc
     6001 gttcacgcgc tcgacgcgtt ccggctcttc gagcatcact tcgagcgcgg ccatcacggc
     6061 ggcgacgttg gacggtggca tcgacgccga gtagatcagc gcgcgggcgt ggtgcttgat
     6121 gtagaatatc acgtcgtcgt cgccggcgac gaagccgccg agcgaggcga acgatttgct
     6181 gaacgtgctc atgatcagat cgacgtgctc ggtcatcccg aagtgatcgg ctgtgccgcg
     6241 accgttgcgc ccgagcacgc cgcaggcgtg cgcgtcgtcg accatcagcc gcgcgccgta
     6301 ggtcttgcag acctcgacga gctgcggcag cggcgcgatg tcgccgccca tgctgaacac
     6361 gccgtcgacg acgacgagga cgcctctgtc gtccgggatc gacttcagga cgcgctcgag
     6421 cgcttcgacg tcgttgtggg ggaagcgctt catctggccg taggacagcc ggcagccgtc
     6481 gacgatgctg gcgtggtcgt ccttgtcgat gacgaccacg tcgccgcgct gcacgatggc
     6541 cgagatcgtg ccgaggttga cctgcatgcc cgtggagaac accagcgcgg cttctttgcc
     6601 gacgaattgg gccagcttgt gctcaagctc gtgatgcagg gccagcgtgc cgttcaggaa
     6661 ccgcgatccg gtgcacgacg tgccgtagcg gtggatcgcg tcgatggccg cttcgcgcac
     6721 cttggggtgt gtggtcagac cgagatagtt gttcgagccg atcatcagga cacggcggcc
     6781 gtccatgatc acttccgtgc cttccgtatc ctgcaacggc ttgaagtacg gatagatgcc
     6841 gctctcgata ctttcgcgag cgatctgcat cagttcggac tcgcgggcct tctggaataa
     6901 gtccatgcgc gaactccttg cggttgagag ataacccggc gcgggcaacg tcggtcatgt
     6961 tgacgagggg tctattggaa ttgtaaccgg gcttaccgga taggtcaagg caagtctctt
     7021 cgacgaggtt gcgaaccatg tgtggccgat ttacactcac cgtcgatccc tcggagctgc
     7081 aacaggcatt cccctggctg aagctgcctg ctgatcccag cttgacgccc cgctacaacg
     7141 tcgcgccgac gcagggtgtc gccggcgtcg ccaacaacag ccagaatacc ctggagatgt
     7201 tccgctgggg gctgattccg gcgtggtcca aggatgccgc catcggcaat cgcctgatca
     7261 acgcgcgcgc ggagacgctg gccgacaagc cttcgtttcg cctggctttc aagcggcgac
     7321 gctgcctgat cctggccgac ggcttctacg agtggcgcaa ggagccgaac ggcaagacga
     7381 agacgccgat gtacattcga ctgaagtcgg gcgagccatt cgccttcgcc gggctgtggg
     7441 aagcgtggcg atcgccggaa gacaagtcga tcctgacatg cacgattatc actacggagc
     7501 cgaacgcgct gctggcgaag atccatgacc ggatgccggt gatcttgaag cccgaggcct
     7561 acatgcgctg gctcgatcct gagccgcgcg agccggaaga gatgagcaag ctgctcaagc
     7621 cctatccggc gtcgcaaatg acggcctacg ccgtgtcgcg ggcggtgaac aacccggcga
     7681 acgattcgcc ggagtgcatc gtcccgatga aatgacaaat gacccatgac gaagggcgaa
     7741 tgtgcgcaga tcgtcattcg tcttccacct gatggaagga gcatgttatg tcgcaggatg
     7801 ccattcaacg cgcgatggag gtgaaacgcc gccacgaaaa agagctgatg cgcaggccca
     7861 acgtcgtcgg ggttggagtc ggatttcgca cgcgtggcgg ggttcgcacc gacgaggcgt
     7921 gcatcgtcgt ttcggtgaag gtcaagctgg ccgcgtcgca gatgaagccc ggcgacctga
     7981 tcccgccgtc gatcgaaggt gtgccggtgg acgtcgtcga aaccggcgag atcacagctt
     8041 tgggcggccg ttaggccgcc cccatctctc tcggggagga atcacatgga tgcgatgcag
     8101 agcgaggcgc tggccgcagc acggcaggtg aagcgcgaga gcgtcgtaac gctgatgaag
     8161 atggcgaacg tcgtcgccac gggcgtcggg tacaagatat ccggtgacac gcagaccggc
     8221 gagctggcgg tggtcgtgtc ggtcaggaag aagctgccgc tggcgcagct cgccgagtcg
     8281 gacatcgtgc ccaagacaat cgacagcgtg aaaaccgacg tgatcgaaac tggagagatc
     8341 gtcgcgtttc aagaccccag ggaaaggatg cggccggcgc gccccggcgt gagcatcggt
     8401 catttcgaga tcacggcagg gacgctaggc tgcctggtgc gcaagaacgg cgaggttcac
     8461 atcctgagca acaatcacgt gttggccaac agcaacaatg cgcaaatcgg cgacgcgatc
     8521 ttgcagccag ggcctgccga cggcgggacg tcggtggatc agatcgggac gttggccgag
     8581 ttcatcccga tcgcgttcga cggcacgctg ccgccccccg gtggctgcct gacgcagttg
     8641 ctttcggcgg tcggcctcca gcccagggca ccgatgcaga acgagccggg taataacaag
     8701 gtcgattgcg cgctggcgcg cccgactacg cctgatctgg tggaccccaa cattctcaac
     8761 atcggcgtgc cgatcggcgc cggcacggcg acgctgggta cgccggtgca gaaatccggc
     8821 cgcaccaccg gctacacgac cggcacgatc ctgcagatcg acgtgaccgt caccgtgaac
     8881 tacggcggac cgttcgccac gttcaccggc caactgatgg ccggcgcgat cagccagggc
     8941 ggcgattccg ggtcggcggt gctggatatg aacaagcgcg tcgtcgggct attgttcgcc
     9001 ggctcgccga cgacgacgat catcaatccg atccagttcg tgctggatat gttgggcgtc
     9061 acgattgtga cgtaggccgt cgccgattct gaaggcagcg gattgcttcg cacaacggat
     9121 aaacggatga gctctgtccc atccgtttat tcgtttcttc aatccgctgc aattccagct
     9181 tcggccgcgc tgtgctagaa tgtgttcgat tcggatcgcc agtagtcgct gccgtcctgg
     9241 cgccggttga tgagaccgag gtcgaacagg tcgcgccgca tggagacgac gtcgggatgg
     9301 aagcgccgga gcagctcgtt gacttccttc tccgtgtagt gccgcccgat ctcgaaatgc
     9361 gacgcgatgt gctcgtacac gatcatcctc ttcttcagtt ggctcggaat ctctttgagc
     9421 gatccatccg ggcgaagata gtcggccagc actttttcga gcgaggtggc tgggggagag
     9481 gctggcgcgc cggaggacag gacacgcttc gcctgcgcgt gcagcgcgtg gtggtcgagc
     9541 gtgtagacgc cgccgctggc ggccacgagt ccggcttcga ccaggcgatc gaggtggcgc
     9601 tgcacgtcga tgcgcggcat gtccagctct gtcgccagcg tttcgaccgt cgcgctcgat
     9661 ctagccagcc ggccggcgat cgcgagccga tcgggatcga ccagcgcctt gaaaaaggcc
     9721 aggagttcat tgagagcatt ttcttccatt gtcatacctc tcatcccgtc gggcgaaatc
     9781 acacggaaac cttagtgact tggtggaaag catatacgat tctaccacgt cgcacgcacg
     9841 gaggcatgca tgagcaagcg acaggttcat accgtgaaga tcgccgggct gacacgcgac
     9901 ttgcccctgt ttgaggtcgc gcccggcgtg cacatcgcca ttttcaacat gctgggcgat
     9961 acggagatcg tgctggccgc ggcgaaggcg ctcgccaggc ggcttcccaa ggacgcccaa
    10021 acgctcgtga ccgccgaggc caagagcatc ccgctggcgc acgcgctatc agtcgagagc
    10081 gggctgccct acgtcgtgct gcgcaagtcg tacaagccgt acatgggcgt cgccgtcagc
    10141 gccgagacgc tcagcatcac caccggccag ccgcagacgc tctacctcga cgagaaggat
    10201 cgggcgctcg tcgacggcag gaagatcgtg ctggtcgacg acgtgatcag caccggcagc
    10261 acgctgcagg gtatgcgcct gatcctcg
//
LOCUS       DBNHXB010000313        10208 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2600563, whole genome shotgun sequence.
ACCESSION   DBNHXB010000313 DBNHXB010000000
VERSION     DBNHXB010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10208)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10208
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2600563"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="AC162G_12590"
     CDS             complement(<1..986)
                     /locus_tag="AC162G_12590"
                     /inference="COORDINATES: protein motif:HMM:NF026240.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076704.1"
                     /translation="MDGQTPVLIRRANKVVLVPIETLIAPEEKREMVTLALGDTEVLT
                     VDSQYRVRFSSASHIIRHVKDRLYTVRYKGGGELKVTGDHSVIVFGENGALVSKRVDE
                     LQVGDYLITHTGADIGQRVNGRVPTGLSVRPEYRTRTRGYRQDVPLNSDLVKLLGYYV
                     AEGHCDYDPPRKLYRVSLTFNLAEESTTAEVKRILAEQFPGIHVTETLRPDSHSRTIM
                     AIGKLIASLCAQFGRTAHEKHVPEWMWELKPDLVRAFLSGYLGDAHILDTEIAFTTVS
                     PRLAHELIYLMRNAGLGCRLYRRVNAPHPSPQGTLIRESVCYDIKVSTRYAHS"
     gene            complement(987..1364)
                     /locus_tag="AC162G_12595"
                     /pseudo
     CDS             complement(987..1364)
                     /locus_tag="AC162G_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257005.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            complement(1361..2317)
                     /locus_tag="AC162G_12600"
     CDS             complement(1361..2317)
                     /locus_tag="AC162G_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR5076705.1"
                     /translation="MRALITGVAGFAGSHLVEYLAAATDWEIWGNVLEADHLRFVVPG
                     AHVVVADLRDPGATREFVEQARPDFVFHLAAQSFVPQSWTDPWDTLETNLRSQINLCE
                     ALLRSSPKARVLVVGSNEEYGQVRSDELPVKEDNPLRPDSPYAVSKIAQDFLGLQYFL
                     SRGLPVVRVRPFNHIGPRQSEKFVVAAFAKQIASIEAGLQPAVVKVGNLNAQRDFTDV
                     RDIVRAYHLAPTQGKVGEVYNIGSGTPRSIQHVLEMLLSYSGVNIRVEIDPERMRPSD
                     TLITYCDASKFTAQTGWTPTIPFEQTLRDVLDDWRKRVREIQ"
     gene            complement(2477..3742)
                     /gene="ahcY"
                     /locus_tag="AC162G_12605"
     CDS             complement(2477..3742)
                     /gene="ahcY"
                     /locus_tag="AC162G_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR5076706.1"
                     /translation="MTVEHHVKNLNLAPGGRYRIEWAEQEMPVLRLIRERFKQDRPLN
                     GLRVSACLHVTTETANLMHTLQVGGADVVLCASNPLSTQDDVAASLVASYEIPVYAIK
                     GEDNQTYYAHIKAALDHRPHMTMDDGADLVSTLHKERTELIDGVVGGTEETTTGVIRL
                     RAMAKEGALKFPIIAINDADTKHLFDNRYGTGQSTLDGVIRATNILIAGKTVVVAGYG
                     WCSRGIAMRAKGLGAHVVVTEVDPLKALEAVMDGFEVMPMIEAAPKGDIFISATGDIN
                     VIDAPHMERMKDGALLANSGHFNVEINIPALEAMSQSKPRRVREFVEQYTVKDGRRIN
                     LLGEGRLINLAAAEGHPASVMDMSFANQALSLEYMAQHGAKLENTVYAVPGDIDRAVA
                     RLKLAAMRVNIDVLTDEQRKYLASWEEGT"
     gene            complement(3888..4667)
                     /locus_tag="AC162G_12610"
     CDS             complement(3888..4667)
                     /locus_tag="AC162G_12610"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5076707.1"
                     /translation="MMRVLSLADPEVIRAFLETDRLWAAYGLADLDPDLYDQSEWYGA
                     EDERGLQSLALLYKGFRLPALFTMGHAQGIALILAAALRAPRVYLNARQEHMLAIQAH
                     YRVHDLEPMWRMTLSPSDFRPVPGRVVMLTPQYTQDLQELYALGGGDAFTPGQVYDGA
                     FYGIDERGRLVAAAGTHVLSERHGAAAVGNVFTHPDYRGKGYATLTTSAVCAELIRRG
                     IRTIVLNVAQANATAIRVYEKLGFKRYLHFNEGVAVRKSTR"
     gene            complement(4678..5670)
                     /locus_tag="AC162G_12615"
     CDS             complement(4678..5670)
                     /locus_tag="AC162G_12615"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR5076708.1"
                     /translation="MNIVVTGSIAYDYIMSFPGYFKDHVLSDKVADSINLSFLVDRMV
                     RRRGGVASNIAYSLALLGERPLLMGSVGQDFGDYRAWLESHGVDTSAVRQYDDVFTAS
                     YFCNIDRAHNQIASFYNGAMARAGEMSLTDLKLAPIDLVVVSPNDPEAMRRYVRECKM
                     MGIPYLYDVSWQIVRLTDDDLREGISGAYVVVVNEYEFEMIRSRTGWSEGDIVERAEV
                     LIVTMGERGSTIHTREEHIHVPAVRTAREVDPIGVGDGYRAGLLKGLALGLGWESAGC
                     IGGLAATYVLENDGPQGHHYTLPEFVERFQLHFADSAATERLLQLAAQDAVREP"
     gene            complement(5686..6525)
                     /locus_tag="AC162G_12620"
     CDS             complement(5686..6525)
                     /locus_tag="AC162G_12620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5076709.1"
                     /translation="MPSSPGSSSAPRRTEDDDLHRVAGAAPAVSFSPNGRRPSAPGTP
                     ARVHRLSIIMCVYNERTSVLPVLERVRQVRPVGGWETEIIVVDNCSTDGTRELLQSLD
                     APDVRVIFHERNMGKGTSVRAGFREATGEYAVIQDADFEYDPADLTRFMAKATETNAD
                     AVFGSRTLGGRAIYKYRKNYFGVRGLTLITNVLYGGRLTDVAVGTKMVKRDVFHALRL
                     RGSAFDLDFELPCRLLKHGYTIVEIPISYRPRTVEEGKKIRAMRDGLHALWVILRERL
                     TRS"
     gene            complement(6503..7855)
                     /gene="rfbH"
                     /locus_tag="AC162G_12625"
     CDS             complement(6503..7855)
                     /gene="rfbH"
                     /locus_tag="AC162G_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HIR5076710.1"
                     /translation="MAEQASRLSDAGGDPVEALRAEILDKVAEYYRLKHAGQPFAPGR
                     TRVQYAGRVFDEQEMMHAVDAVLDFQLTHGRFGREFERKLGAFLGVREVIPVNSGSSA
                     NLVAMTTLCARQLRNGLRPGDEVITPAVTFPTTLAPIVQNRLVPVMVDCQLGNYNVDV
                     DHLEEARSDRTRAMFVPHTLGNPVDMDRVTAFARKHDLYLIEDTCDALGSTFGGRLVG
                     TFGHFGTLSCYPAHHITTGEGGVVFTNSLRLARLARTIRDWGRDCFCGYDNPPDGKCG
                     HRFDWKIAGPGESYDHRYLYTEIGYNLKMTDRQAAIGVAQLDKLPSFIEKRKKHFRLI
                     EAGLKRYEEHLILPMHHPKADPSWFAFPITVRPGAPFTRKQITRFLEDRNIETRYLFS
                     GNILEQPGYRDIERRVVGELPNSDVVLRGAFFVGVYPGLDETRIEYMLDAFHAFFARL
                     "
     gene            complement(7831..8760)
                     /locus_tag="AC162G_12630"
     CDS             complement(7831..8760)
                     /locus_tag="AC162G_12630"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5076711.1"
                     /translation="MFDLHGTRVLVTGATGFIGAHLARRLVAAGAQVVAFRRFSLESQ
                     RKHERLREVQVEWRDVDLRTFGAVSSAVRVARPELVFHLAAEGVTDPFLSPDAAIRAN
                     VYGAIHLLRAVDGKAPVVVARTPGERDAMNVYTASKAAAWSFCRMYRRTHGWSIAGAM
                     PFQTYGPGQPAAALVPSAIEAAMRGEDFPMTHGEQQRDWIFVEDVVDALIRIGSRLLA
                     PDSLMAEDAIEIGTGRATSVREVVESIYRRVGGAGRPRVGVLPARPGEVDRQVADAGR
                     THELIGWRAQFTLEEGLRETIQWTREWLNKLAG"
     gene            complement(8769..9794)
                     /locus_tag="AC162G_12635"
     CDS             complement(8769..9794)
                     /locus_tag="AC162G_12635"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017658996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR5076712.1"
                     /translation="MSNEFWKGRRVFVTGCTGILGSWLTQALVTRGANVVGLIRDRVP
                     NSDLFRSGTAARVTQVDGDIVDFQLLGRVLNEYEIDTVFHLAAQALVRVANRMPVGTF
                     ETNVRGTWLLLEACRDVKTITRIVVASSDKAYGDQDVLPYSEDAPLQGRHPYDVSKSC
                     ADLIARAFACTYDMPIAVTRCGNIYGGGDLNWDRIVPGTMRSVLRGEPPIIRSDGSPL
                     RDYLYVDDIVDGYLRLSERLDDRSLHGQAFNFGMDDPKSALEIVESIISISDHPGLKP
                     LILNNVSHEIQNQYLSSEKARRLLGWQPRYTLAEGLRRSMEWYRAYLSETEGLSKMPG
                     ILQASQV"
     gene            complement(9946..>10208)
                     /locus_tag="AC162G_12640"
     CDS             complement(9946..>10208)
                     /locus_tag="AC162G_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HIR5076713.1"
                     /translation="VTGFREKPQVEGYVNGGFFVFERGVLDYLSGPDDLELEKEPLQN
                     LARDGQLCVFRHDGFWRAMDTFKDVQALAAIWERGEAPWKVW"
BASE COUNT         1937 a         3320 c         3005 g         1946 t
ORIGIN      
        1 gagtgggcat aacgtgtgct gactttaatg tcgtaacaaa ccgattcccg gatcaaagtt
       61 ccctgcgggc ttggatgagg cgcgttcact cttcggtaca agcgacatcc tagcccggca
      121 ttacgcatca aatagatcaa ctcgtgagcc aggcgcgggc tgacggtcgt gaaagcaatt
      181 tcggtatcca gaatatgcgc atctccaaga taaccgctga ggaaagcgcg aaccaaatcc
      241 ggtttcaact cccacatcca ctcgggtaca tgtttctcat gtgcggttct gccgaattgc
      301 gcgcacagac tagcgatgag tttgccgatc gccataatgg ttcgcgagtg gctatccggt
      361 ctaagggttt cggtcacgtg tatgccgggg aattgctcag ccaggatccg cttaacctcc
      421 gccgtcgtgc tctcttctgc caggttaaat gtcaacgata cgcggtacag cttgcgtggc
      481 ggatcatagt cgcaatgacc ttcggcgacg taataaccca gtaacttcac cagatccgaa
      541 ttcaagggca catcttgtcg ataaccgcgc gttcgagtgc ggtattccgg gcggacgctt
      601 aacccggtcg gcacgcggcc attcactcgc tggccgatat ccgcgccagt atgggtgatc
      661 agatagtccc ccacctgcag ctcatccacg cgcttcgaaa ccaacgctcc attctcacca
      721 aagacaatga ccgaatggtc accggtcacc tttaattcac cgcccccctt gtatctcacc
      781 gtgtacaagc gatccttcac gtggcgaata atatgactcg ccgacgagaa gcgcacacga
      841 tattgcgagt ccactgtgag aacctcggta tctccgagcg ccagagttac catctcccgc
      901 ttttcttccg gcgcgatcaa agtctcgatg ggaaccaaca cgaccttgtt ggcccgacga
      961 atcaacacgg gcgtttgacc atccacgcta ctcgacgcct ggtagaaccg gatagtcgga
     1021 tcgacgacgc gtatcgcatc gagcaggcgc gtcacgccga gcgccgtcgc ttcgcccgtc
     1081 agcaccggct gctgccacga ggtcggcacg aaagacatcg cggccaggtt atatacctcg
     1141 tccgggtgga actcgcgcag gatgccgatg agcgacgcct cgtcgagcag atcgccttgc
     1201 accagcgtga tgcgatcctg aatgtgctcg atgcgatcga ggttgatgat gctggtgcgg
     1261 cgcaccatcc ccacgacatc gtagcccttt tccaaaagga attcggccag gtacgagccg
     1321 tcctgaccgg ttacacccgt gatcagtgca acggatttgc tcattggatt tcccttactc
     1381 tctttcgcca atcatcgagc acgtcgcgca atgtctgctc gaacgggatc gtgggagtcc
     1441 agccagtctg agccgtgaac ttgcttgcgt cgcagtaggt gatgagcgta tcggaggggc
     1501 gcatgcgctc gggatcgatc tccacccgga tgtttacacc gctgtagctc agcagcatct
     1561 ccagcacgtg ctgtatgctg cgcggcgtgc cggagccgat gttgtaaact tcgcccacct
     1621 tgccctgcgt cggcgccaga tggtacgcgc gcacgatgtc gcgcacgtcc gtgaagtcgc
     1681 gctgcgcgtt caagttgcct accttgacaa cggctggctg aagtccggct tcgatcgacg
     1741 cgatctgttt cgcgaaagcg gcgacgacga acttctcgct ttggcgaggt ccaatgtgat
     1801 tgaacggtcg cacgcgcacg accggcaatc cccggctcaa gaaatactgg aggccgagaa
     1861 agtcttgcgc gatcttgctc acggcgtacg ggctgtccgg cctgagcgga ttgtcctctt
     1921 tcacgggcag ctcgtcgctg cgcacctgcc cgtactcttc attcgagccg accaccagga
     1981 cgcgggcttt cgggctgctc cgaagaagcg cctcgcacag gttgatctgc gagcgcaaat
     2041 tcgtctcaag cgtatcccag ggatccgtcc acgactgcgg gacaaaggac tgtgccgcca
     2101 ggtgaaagac aaaatcgggg cgggcttgtt caacgaattc acgtgtcgca cctggatcgc
     2161 gcaaatcggc gaccacaacg tgggcaccgg gcacgacaaa gcgcaagtgg tcggcttcga
     2221 gaacgtttcc ccagatctcc cagtcggtgg cagcggccag atactcgact agatgactac
     2281 ccgcaaagcc ggcgaccccc gttatgagcg ctcgcaaacg tcgcctcaag taaacgtgat
     2341 gaatttctca ataattatac ccaagttcac aaggagggca aacgattcga tgaaaggaca
     2401 accaaaagag cctcacttcc gcgaggctct gtcggcctgt ggccttcgtc gagcgttgct
     2461 cgccagacgc actgacctac gtgccttctt cccaagacgc caggtacttc ctctgctcgt
     2521 cggtcagcac gtcgatgttc acgcgcatcg ccgcgagctt gaggcgcgcg actgcgcggt
     2581 cgatgtcgcc cgggaccgcg tagactgtat tttctagttt cgcgccgtgc tgcgccatgt
     2641 actcgagcga cagcgcctgg tttgcaaagg acatgtccat cacgctggcg gggtgtccct
     2701 cggcggcggc caggttgatc agccgacctt cgcccagcag gttaatcctg cggccatcct
     2761 tgaccgtgta ctgctcgacg aactcgcgca ctctacgcgg tttactctgc gacatcgcct
     2821 ccagcgccgg gatgttgatc tcgacgttga agtggccaga gttggcgagc agcgcgccat
     2881 ctttcatgcg ctccatgtga ggcgcatcga tcacgttgat gtcgccggtc gcgctgatga
     2941 agatgtcgcc cttcggcgcg gcttcgatca tcggcatcac ttcgaaacca tccatcaccg
     3001 cctcgagcgc cttgagcgga tcgacctccg tgacgacgac gtgcgcgccc agtcccttgg
     3061 cgcgcatggc gatgccacgg ctgcaccagc cgtacccggc gacgacgacg gtcttcccgg
     3121 cgatcaggat attcgtcgcg cgaatcacgc cgtcgagcgt cgactggccg gtgccgtagc
     3181 ggttatcgaa caggtgcttc gtgtcggcat cgttgatcgc gatgatggga aacttgagcg
     3241 cgccttcctt cgccattgct cgaagccgga tgacgccggt ggtcgtttcc tctgtcccgc
     3301 ccacgacgcc gtcgatcagc tcggtgcgct ccttatgtag tgtcgagacc agatcggcgc
     3361 cgtcgtccat cgtcatgtgc ggcctgtgat cgagcgcagc cttgatgtgc gcgtagtagg
     3421 tttgattgtc ctcgccctta atcgcgtaca ccggtatctc gtaactggcg acgagcgacg
     3481 cggcgacgtc gtcctgcgtg gacagcgggt tcgaggcgca caagacgacg tccgcaccgc
     3541 ccacctgcag cgtgtgcatc aggttcgccg tctcggtggt cacgtgcagg cacgcgctga
     3601 cgcgcagccc gttcagaggc cggtcttgct tgaaccgctc gcggatgagc cgcaagacag
     3661 gcatctcctg ctcggcccac tcgatgcggt atcgcccgcc aggggccagg ttgagattct
     3721 tgacgtgatg ctcgactgtc atcttggttg tgactccttg tcggtaggtt gagagctgga
     3781 ttttggcgaa tctccggagt cgagccttta ggcggtcatc tctggcggtc aaaaccggct
     3841 aaagcctcga ctccggctaa gggttggcgg acgggtgaga gggtggatca acgtgtcgac
     3901 ttgcgcacgg ccaccccttc gttgaagtgc aggtatctct tgaagcccag tttctcgtac
     3961 acgcgaatgg ccgtggcatt cgcctgcgcc acattcagca cgatggtgcg gatgccgcgc
     4021 cggataagct cggcgcacac ggcgcttgtc gtaagcgtcg cgtagccttt cccgcgataa
     4081 tccggatgcg tgaagacgtt gccgacggca gccgcgccgt gcctctcgct caagacgtgc
     4141 gtgccggctg cggcgaccag cctcccgcgc tcgtcaatgc cataaaacgc gccatcgtac
     4201 acctggccgg gcgtgaacgc atcgccgccg ccaagcgcgt acaactcctg caggtcctgg
     4261 gtgtactgcg gcgtcagcat gaccacgcgt cccgggacgg gccgaaaatc gctcgggctg
     4321 agcgtcatgc gccacatcgg ctcgagatcg tgaacgcggt aatgcgcctg aatggccagc
     4381 atgtgttcct ggcgggcgtt caaatagacg cgcggcgcac gcagcgccgc agccaggatc
     4441 aacgcgatcc cctgcgcatg acccatcgtg aacagggcag gcagcctgaa ccctttgtag
     4501 agcagcgcca gcgactgcag gccacgctca tcctctgcgc cataccattc cgattgatca
     4561 taaaggtcgg gatcgagatc ggccagccca taggccgccc acaggcgatc ggtctccaga
     4621 aaggcgcgta tcacttccgg atcggcgagc gaaagtaccc gcatcatcca tccgcgttca
     4681 cggctcgcgc acggcgtcct gcgcggcgag ctgcagcaat cgctcggtcg cggccgagtc
     4741 ggcgaaatgc aactgaaacc gctcgacgaa ttcgggcagc gtgtaatgat gtccctgcgg
     4801 gccgtcattt tccagcacgt acgtcgccgc cagcccgccg atgcagcccg ccgattccca
     4861 acccaggcca agcgccagcc ctttgagcaa accggcccga tacccatcac cgacgccgat
     4921 cggatcgacc tcacgcgccg tcctgaccgc cggcacgtga atgtgctcct cgcgtgtatg
     4981 aatggtcgag ccacgctctc ccattgtcac gatcagcact tccgcgcgct ccactatgtc
     5041 gccctcgctc caaccggtgc ggctgcggat catttcgaac tcgtattcgt tgacgaccac
     5101 gacgtacgcg ccgctgatgc cctcgcgcaa gtcgtcatcc gtcaggcgca cgatctgcca
     5161 gctcacatca tagagatagg ggatccccat catcttgcat tcgcgcacat agcggcgcat
     5221 ggcctcggga tcgttggggg atacgacgac caggtcgatc ggcgcaagct tgagatcggt
     5281 cagcgacatc tcgcctgcgc gcgccatcgc accattgtag aagctcgcga tctggttgtg
     5341 cgccctgtcg atgttgcaga aataggatgc cgtgaacacg tcgtcgtact ggcgcacggc
     5401 cgaggtatcc acgccgtgcg actcgagcca ggcgcgataa tcgccgaaat cctggccgac
     5461 acttcccatc agcagcggcc gctctcccag caaagccagg gagtaggcga tattgctggc
     5521 cacgccgccg cgccggcgca ccatgcgatc gaccaggaaa ctcaggttga tggagtccgc
     5581 gaccttgtcc gaaaggacat ggtccttgaa atagcctgga aacgacatga tgtagtcgta
     5641 ggcgatcgat ccggtgacga cgatgttcac gtgcacaccc ctcacttagc tccgcgtcaa
     5701 tcgctctctc agaatcaccc acagcgcgtg caggccgtcg cgcatagcgc ggatcttctt
     5761 gccttcctcc accgtgcgcg ggcgatacga gatgggaatc tccacaatcg tatagccgtg
     5821 cttgagcagc ctgcacggca gctcgaagtc gaggtcgaag gcgctgccgc gcaggcggag
     5881 cgcatgaaac acgtcgcgct tcaccatctt cgtgccgacg gccacatccg tcagtcgccc
     5941 gccgtacagc acattcgtga tcagcgtcag gccgcgcacg ccgaaataat tcttgcggta
     6001 tttgtagatc gcccggccgc cgagcgtgcg cgagccgaag acggcgtcgg cgttggtctc
     6061 tgtcgctttc gccatgaacc gcgtcagatc tgcaggatcg tactcgaaat ccgcatcctg
     6121 aatcacggcg tactcgccgg tcgcttcgcg gaagcctgcc cgcaccgacg ttcccttgcc
     6181 catgttacgt tcgtggaaga tcacccgcac atccggcgcg tcgagcgact gcagcagctc
     6241 gcgcgtcccg tccgtcgagc aattgtcgac gacgatgatc tctgtctccc agccacccac
     6301 cggccgcacc tggcgcacgc gctccaggac cggcagcacg ctcgtccttt cgttgtagac
     6361 acacatgatg atcgacagcc ggtggacgcg cgcgggcgtg ccgggtgcac tggggcgccg
     6421 cccgttcggc gagaaagaca cagcgggcgc ggctccggcg acccgatgga gatcgtcatc
     6481 ctcagtgcgc cggggcgcag agctagagcc tggcgaagaa ggcatggaag gcatccagca
     6541 tgtattcgat gcgcgtctcg tccagtccgg gatatacgcc gacgaaaaat gctcccctca
     6601 aaacgacgtc cgaattggga agctctccga cgacgcgtcg ctcgatgtcc cggtagccgg
     6661 gttgctccag aatgttgcct gagaacaggt agcgcgtctc gatattgcga tcttccagaa
     6721 accgagtgat ctgcttgcgg gtgaacggcg cgccagggcg caccgtgatc gggaaggcga
     6781 accaggatgg gtcagccttg gggtgatgca tgggcaggat cagatgctcc tcgtagcgtt
     6841 tcagcccggc ctcgatcagg cggaagtgct tcttgcgctt ctcgatgaac gacggcagct
     6901 tgtcgagctg cgccacgccg atggcggcct gtcgatcggt catcttaaga ttgtatccga
     6961 tctccgtata caggtaacgg tgatcgtacg actcacccgg gccggcgatt ttccagtcga
     7021 atcggtgacc gcacttgccg tccggcggat tgtcgtagcc gcagaagcag tcgcgtcccc
     7081 agtcacggat cgtgcgtgcc aggcgggcca ggcgcaggct gttggtgaag accactccgc
     7141 cttcgccggt cgtgatgtga tgagccgggt agcaactgag cgtgccgaaa tggccgaagg
     7201 tccctaccag cctgccgccg aacgtgctac ccagcgcatc gcacgtgtcc tcgatcaggt
     7261 acaggtcgtg cttgcgggcg aaggcagtga ctctgtccat gtcgaccggg ttgcccagtg
     7321 tgtgcgggac aaacatcgcc cgggtccggt cagaccgggc ctcttcgagg tgatcgacgt
     7381 cgacgttata gttgcccaac tggcaatcga ccatgacggg caccaggcgg ttctgcacga
     7441 tcggagcgag cgtcgtgggg aacgtgaccg ccggcgtgat cacctcgtcg ccggggcgca
     7501 agccgttgcg caactgccgc gcgcacagtg tggtcatcgc gaccaggttc gccgacgagc
     7561 ctgagttgac cggtatgacc tcgcgaaccc cgagaaaggc gcccagcttc cgctcgaact
     7621 cgcggccgaa gcgaccgtgc gtcaactgaa agtcgagcac ggcgtccacc gcgtgcatca
     7681 tctcctgctc gtcgaacacg cggcccgcat actgcacacg cgtccggccc ggcgcaaatg
     7741 gctggccggc gtgcttcaag cggtagtatt cggcgacttt gtccaggatc tccgcacgaa
     7801 gcgcctcgac cggatcaccg ccggcgtcgc tcagccggct agcttgttca gccactcgcg
     7861 tgtccattga atcgtctctc gcaagccttc ttccagcgtg aattgggcgc gccagccgat
     7921 caactcgtga gttcgtccgg catccgcgac ctgccgatcc acctcgcccg gccgcgccgg
     7981 cagcacaccc acccgcggcc ggcccgctcc gcccacccgc cgatagatcg actccaccac
     8041 ttcgcgtaca gaggtcgccc ggcccgtccc gatctcaatc gcatcttcag ccatcaagga
     8101 gtccggcgca aggagtctgc ttccgatacg gatcaacgca tcgacgacgt cttcgacgaa
     8161 tatccagtcg cgctgctgct cgccgtgcgt catcgggaag tcttcgccgc gcatcgcggc
     8221 ttcgatcgcc gacggcacaa gcgcagcagc cggctggccc ggcccgtacg tctgaaacgg
     8281 catcgcaccg gcgatcgacc agccgtgcgt gcgccgatac atcctgcaaa acgaccaggc
     8341 cgccgccttc gacgccgtat acacgttcat cgcgtcgcgc tcgcccgggg tgcgcgcgac
     8401 gacgacgggc gccttgccgt ccacggcgcg cagcaggtgg atcgcgccgt acacattggc
     8461 gcgtatggcc gcatcgggcg acagaaacgg atcggtcact ccttcggcgg cgaggtgaaa
     8521 caccaactcc gggcgcgcca ccctcacagc gctcgagact gcgccgaacg tgcgcaggtc
     8581 gacgtcgcgc cactccacct gaacttcgcg cagcctctca tgcttccgct gagactcgag
     8641 gctgaagcgc cgaaaagcga cgacctgcgc gccggctgcg accaacctgc gggcgagatg
     8701 tgctccgatg aagccggttg cgccggtgac cagcacgcga gtgccatgca ggtcgaacat
     8761 gtcgctcgct agacttgcga cgcctgcaag attcccggca tcttcgacag gccctctgtt
     8821 tccgacaggt aagcgcgata ccactccatc gacctgcgca agccctctgc gagtgtgtaa
     8881 cgcggctgcc agcccagcag gcgccgcgcc ttttcgctgg acaggtactg attctgaatc
     8941 tcgtggctga cgttgttcaa gatcagcggc ttcagcccag ggtggtcgga gatggaaatg
     9001 atcgattcga cgatctccag cgccgacttt ggatcgtcca tcccgaaatt gaacgcctgt
     9061 ccgtgcagcg agcggtcgtc cagccgctcg gacaggcgca ggtaaccgtc gacgatgtcg
     9121 tcgacgtaca gataatcgcg caggggcgat ccgtcgctgc gaataatcgg cggctcgccg
     9181 cgcagaacgc tgcgcatcgt gccgggcacg atccgatccc agttgagatc gcccccgccg
     9241 tagatgttgc cgcagcgcgt gacggcgatc ggcatatcgt acgtgcaggc aaacgcccgc
     9301 gcgatcagat cggcgcacga cttcgaaacg tcgtagggat gtcgcccttg caacggcgca
     9361 tcctcggaat acggcagcac gtcttgatcg ccgtaggcct tgtcggagga ggcgacgacg
     9421 atgcgcgtga tggtcttcac atcgcggcac gcctccaaga gcagccacgt gccgcggacg
     9481 ttggtctcga acgtgccgac cggcatgcgg ttcgccacgc gcaccagggc ctgtgccgcg
     9541 agatgaaaca ctgtgtcgat ctcatattcg ttaagcacac gcccgagcag ttgaaagtca
     9601 acgatgtcgc cgtccacctg cgtgacgcgc gctgcagtgc ccgatcgaaa cagatcgctg
     9661 ttcggcacac ggtcgcgtat cagcccaacc acgttggcgc cgcgtgtgac gagcgcctgc
     9721 gtcagccacg agcctagaat cccggtgcaa cccgtgacga aaacgcgacg acctttccag
     9781 aactcgttgc tcaacaagac tgcctctctt cgtcgactgg ttgcatagtc gtttagctta
     9841 ttttccgcag ttcccgccgc agttggactg cggcggcttc gaacggcagt gcaactgccg
     9901 ttccaacatt cggcgacacg accaagcggc catgtgacta tctgactacc agaccttcca
     9961 gggagcctcg ccgcgctccc agatagctgc cagcgcctga acatccttga acgtatccat
    10021 ggcgcgccag aacccatcgt gccgaaacac gcacagttgg ccgtcgcgag ccaagttttg
    10081 aagcggctcc ttctccagct cgagatcgtc cggtccactc aggtagtcga gcacgccgcg
    10141 ctcgaacacg aaaaacccgc cgttgacgta gccttcgacc tgcggctttt cgcgaaatcc
    10201 cgtcacca
//
LOCUS       DBNHXB010000314         7793 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1427563, whole genome shotgun sequence.
ACCESSION   DBNHXB010000314 DBNHXB010000000
VERSION     DBNHXB010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7793)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1427563"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1208)
                     /locus_tag="AC162G_12645"
     CDS             complement(90..1208)
                     /locus_tag="AC162G_12645"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5076714.1"
                     /translation="MKRFLTLLSITLFVAACGAPAPTFAPTSTTQPAPAPTHTPIPSP
                     TPSPAPTATATPSQTPAPTATPDPFAPFTIERLREREYDAGPIEIVETLEDNAAFTRY
                     GVAYPSDGLRVTAMMNVPKGSGAPGETFPVIILNHGYVDPQSFPTGSYIRAEADYLAR
                     LGYLTLSPDYRGHAGGGGDSEAEGASVRGFNTFRVAYAVDVLNLLNAVPSIPQADPTR
                     IGMWGHSMGGGVTLKVLTVDRGESVDAAVLYGAMSGDEAANLRHIDRMWRPGLYDLVT
                     STFGAPEERPEDYARLSSLTYVADVTAPVNIHHGALDAQVPLAWSQDLAQRLADAGKS
                     VEYFEYAGAGHSLRGDDWSVFMQRVAAFFDQYVKGVNE"
     gene            complement(1384..1456)
                     /locus_tag="AC162G_12650"
     tRNA            complement(1384..1456)
                     /locus_tag="AC162G_12650"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Asn,seq:gtt)
     gene            complement(1630..2532)
                     /locus_tag="AC162G_12655"
     CDS             complement(1630..2532)
                     /locus_tag="AC162G_12655"
                     /inference="COORDINATES: protein motif:HMM:NF017652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HIR5076715.1"
                     /translation="MQLKAALNGARSGMEHSALPVTADAIARDAQAVVAAGAAAVHFH
                     IRGPNGRESLAPEDVAHCITACRAAVPAIPLGVSTGTWIVPDLAARLALIRAWQTLPD
                     FASVNFDEEGAEEGGLTMSRLLRIVLVMLVPLVLVLVVALSVYKPGPPDKVVTAVYGY
                     LRYQESSSQQAGAVQQIVHAARPWNLTPQMSRATFGSSVVYQTTTNYDVPGSFGLRAT
                     ATPWPREFSSRRGGLRPLPFPPVDVWCVLLRQAGDASPGVVFVAEHHDIYNADWILHE
                     PNAESRPELNAILSSVGCDLVLDR"
     gene            complement(2778..3377)
                     /locus_tag="AC162G_12660"
     CDS             complement(2778..3377)
                     /locus_tag="AC162G_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076716.1"
                     /translation="MHMKINRYQILGSVIMLLFLSAILVGIPAFQHQGWIDFPCSVRY
                     VRKDDCLLPINLEGWRPPQIESALDRYEAGWSGEACDYHTSDTRVLFVQTPILNLSLE
                     IVDDTLKVNGSILDKGAEFQAINILNWNPWRISRIRFENLGLLPYCDSRVEGKTLQVY
                     GRYGTEVSPIKGVLLLCALAGGQLLVYRKSRQAAKHNAA"
     gene            complement(3428..4213)
                     /locus_tag="AC162G_12665"
     CDS             complement(3428..4213)
                     /locus_tag="AC162G_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR5076717.1"
                     /translation="MASTSQNTLILASTWLREYNTCQRDANLKAGTRVTAILEFLRQA
                     PLFAGLSDTELESVARDFVTRQFRQGETIFLQGDAGQLLYLIRSGRVRVFVVGEEGQE
                     TSVILYGPGDIFGELAVIDEMPRSASVAAMEDTAVLTLTRDQLREHMRREPQLALNFL
                     KALSARLRYSTQQVETLTFLDVPARLARKLLQLAHEYGVAEEGGVRINSPLTQSDLAS
                     LIGATRESINKAMGAFRRRGLVNTVQGYIVIVDPDALREEVRS"
     gene            complement(4228..4920)
                     /locus_tag="AC162G_12670"
     CDS             complement(4228..4920)
                     /locus_tag="AC162G_12670"
                     /inference="COORDINATES: protein motif:HMM:NF023178.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HIR5076718.1"
                     /translation="MVLAMYLSHLIADYVLQWDRLALWKSREYKGVVVHGLVVLAVTW
                     ACTLPFDAGWWPWVLFVWLTHTAVDAIRLRLGKAFSALALFVLDQAVHLSVITFALVE
                     SGYLAAPARLADLAPVLLTDRLLVFAIGLVFVTAPAWVLVEFVVYGLLKGSGPDFSQA
                     PNKYVSSLERALMTVSVLLGQIALVPLVALPRLAMEWRQVAGSPRATVYVAELLASVA
                     LAVAVGLGLRLV"
     gene            5153..6139
                     /locus_tag="AC162G_12675"
     CDS             5153..6139
                     /locus_tag="AC162G_12675"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF021507.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HIR5076719.1"
                     /translation="MELALWTVALCLLVTHIRPLTWARKATGRAQRSIRVASVGLLLI
                     LAVLLAASGAYSFWIAHRPLPGNTRQVVFEGITYIREVRRSPRPIVIHVMRVELDAPG
                     IRFLVTPGDPSPEDEIRARTTSQFVAEFDLQVAINGDFFTPWWSYGPWDYYPRVGDPI
                     DVKGFASSNGAVYSGGESNDGPTLYLSAGNQASFDVPIGSVYNAISGNTIFLEDGVAR
                     VEPPEYHFEPHPRTAIALDRARRTLILVVVDGRQPNYSEGISLAELAQILRDYGGDTA
                     LNLDGGGSTTMVIEGDNGEPVILNSPIDNLIPGRERPVANHLGIYAHPASAP"
     gene            complement(6238..6311)
                     /locus_tag="AC162G_12680"
     tRNA            complement(6238..6311)
                     /locus_tag="AC162G_12680"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6275..6277),aa:Lys,seq:ctt)
     gene            6577..7572
                     /locus_tag="AC162G_12685"
     CDS             6577..7572
                     /locus_tag="AC162G_12685"
                     /inference="COORDINATES: protein motif:HMM:NF014898.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR5076720.1"
                     /translation="MSAEQLSLFDGSSQPEPAPAEGETGVDLSVARALTSRTRLSVAM
                     EAYRDHMIARQFSENTVKAFMYDLHLVAEFFTPIKPIGEVAHRDLERFVDWLVTGRGV
                     PCKPKSLARRITTLKVFFGWLHEIGAIPVDPAAPIVHRPVTTPLPDILFDNEIDAVLA
                     ATQRLRAGVASEPGAPPRPDARPHLLVTLLLSTGIKKSECVAIKLEHIDLSDAGDPAL
                     WIRYADARHRHKERKLRLDPRWPKVLDEYRTQYDVKELIFPWSARNLEYVLTDVGKMA
                     GLTKPLSFETLRWTCAVTDKRKGMPDETLRRKMGLSQISWREVGEKLVKLAAPAL"
     gene            complement(7600..>7793)
                     /locus_tag="AC162G_12690"
     CDS             complement(7600..>7793)
                     /locus_tag="AC162G_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076721.1"
                     /translation="HNIWPDDAARFASDLLMPEVIDDPFLREFTLAAAGCPIPILLGG
                     HSLVTAGLWVLVEGVTSDA"
BASE COUNT         1549 a         2505 c         2365 g         1374 t
ORIGIN      
        1 gtcagccttc atccgtttat ccgttgacac cagcgcatcc tgattgctcg gaatcggcac
       61 cagcgagcct tcatccgttt atccgttgcc tattcgttga cacccttcac gtattgatcg
      121 aaaaacgcgg cgacgcgctg catgaagacc gaccagtcgt cgccgcgcag cgagtggccc
      181 gcgccggcat attcgaagta ttccaccgat ttgccggcat ctgccagccg ctgcgccagg
      241 tcttgcgacc aagccagcgg gacctgagcg tcgagcgcgc cgtggtgaat gttgacgggc
      301 gcggtgacgt cggcgacgta cgtcagcgac gagaggcggg cgtagtcttc gggtcgttcc
      361 tcaggagcgc cgaacgtcga ggtcaccaga tcgtacaggc cgggccgcca catgcgatcg
      421 atgtggcgca ggttggccgc ttcgtcgccg ctcatcgcgc cgtagagcac cgccgcgtcg
      481 accgactcgc cgcgatcgac ggtgagaacc ttcagcgtga cgccgccgcc catggagtgc
      541 ccccacatgc cgatgcgggt cggatcggcc tgcgggatcg aggggacggc gttgagcaga
      601 ttgagcacat ccactgcgta ggcgacgcgg aaagtgttga acccgcgaac gcttgcccct
      661 tctgcttccg aatcacctcc cccaccggca tggccgcgat aatcgggact gagcgtcaga
      721 tagccgagcc gcgccaggta gtccgcctcg gcgcgaatgt agctgcccgt cgggaaactc
      781 tggggatcga cgtagccgtg attgaggatg atgaccggaa acgtctcgcc cggcgcgccg
      841 ctgcccttcg gcacgttcat catcgccgtc acgcgcaggc cgtcacttgg gtaggccacg
      901 ccgtatcgcg tgaatgccgc gttgtcttcg agcgtttcga cgatctcgat cgggcctgcg
      961 tcgtactcgc gctcgcgcag cctttcgatc gtgaacggtg cgaagggatc cggggtggcg
     1021 gtcggcgccg gcgtctgcga aggcgtcgcc gtagcggttg gcgccggcga cggcgtgggc
     1081 gaggggatcg gcgtgtgagt cggcgccggc gccggttggg tggtcgacgt cggcgcgaaa
     1141 gtcggcgcag gcgcgccgca ggcggcgacg aaaagcgtaa tcgagagcag agtgaggaat
     1201 cgtttcatat acccagtata actcagactt cggaagtctc agggcgtgtt ctcgcagggg
     1261 acggtggccg gaaagccaag tcgcttcgct ggcgagatgc acttaaagac ttccgaagtc
     1321 tcggaactgg gaaatccgcc aggggaacaa aacggggaag cacttgcttc cccttgcgtc
     1381 aggctcctcg gctgggactc gaacccagaa cccatcggtt aacagccgat tgctctgccg
     1441 attgagctac cgaggatcgc agcgcacacg attataccag tggggatagg cagcgtcaag
     1501 aataacgcga atgggtctgc acacaagatg tcaggccgga cgtagggcgg ctttccatag
     1561 ccgccaagtc gcggcgcgca tggaaacgcg ccctacggtg gatgacaacc tttccgtgca
     1621 tacgcgcgct caccgatcca gaaccaggtc gcagccgaca ctcgaaagaa tcgcgtttag
     1681 ctcgggccgg ctctcggcgt tcggctcgtg caggatccag tcggcattgt agatgtcatg
     1741 gtgctccgcg acgaagacga cacccggcga ggcatcacct gcctggcgga ggagcacgca
     1801 ccacacgtcc acgggcggga acggcaaggg gcggagaccg ccgcgccggg aagagaattc
     1861 gcgcggccac ggcgtcgccg ttgcgcgcaa tccgaaactg cccggtacgt cgtagttggt
     1921 ggtggtctgg taaactacac tgctgccgaa cgtagccctg ctcatctgcg gcgtgaggtt
     1981 ccagggccgc gcggcgtgca cgatctgctg aacggcgccg gcctgctgcg acgatgactc
     2041 ttggtatcgc aggtacccgt agacggcggt cacgacttta tcgggtggcc ccggtttgta
     2101 aaccgacaag gccacgacga gcacgagcac tagaggcaca agcatgacta atacaattct
     2161 caacaggcgg ctcatggtga gacccccttc ctctgcacct tcctcgtcga agttcaccga
     2221 cgcgaagtcg ggcagagtct gccaggcgcg aatcagcgcc agtcgcgccg ccaggtcggg
     2281 gacgatccac gtcccggtgc tcaccccgag tgggattgcc ggcacggctg cgcggcaggc
     2341 ggttatgcag tgcgcaacat cctctggcgc cagactttca cgcccgttgg ggccgcggat
     2401 gtggaagtga acggctgcgg cgccggcggc gacaaccgcc tgcgcatcgc gcgcgatcgc
     2461 atcggcggtg acgggcaggg ctgagtgctc cattccggag cgggcgccat tgagcgcggc
     2521 cttcagctgc attcaccttt tcaaagtatg caattctggc acaccggata tatagcgacg
     2581 gaagcgagcg caggctaacg gcagagccat tctgccgacg tgatgcccga gactacctca
     2641 caggtaaggc gcgaccgata agcccgaacg acggcaggcc agatacgaca gatgaacacg
     2701 atacctcacg gcgagtctac cttgccaatc tgagcgcctg attcgcggcg cggtagtcgg
     2761 tcggcttcgg cgacgggtca ggcggcgttg tgtttcgccg cttgccttga cttccgatac
     2821 accaggagct gtccgccagc caatgcacaa agcaggagta cgcctttgat aggcgaaacc
     2881 tcggtgccgt atctgccata tacctggagt gtcttgccct ccacccgcga atcacagtaa
     2941 ggcaacaggc cgaggttctc gaatctgatg cgggagattc tccatggatt ccagttgaga
     3001 atattaatgg cttggaattc cgccccttta tcgagaattg agccgttgac cttcagggta
     3061 tcgtcaacta tctcaagact cagattcaat atcggtgtct gaacaaacag aacacgggta
     3121 tctgatgtgt ggtaatcgca ggcttcaccg ctccatcccg cctcgtatct gtcgagggct
     3181 gactcaatct gaggcggtct ccaaccctct aagttaattg gaagcagaca atcatctttc
     3241 ctaacgtacc gcactgagca tgggaaatca atccatcctt gatgctggaa agctgggata
     3301 ccgacgagaa tagcggagag gaatagaagc attatgactg agccaagaat ctgatagcga
     3361 ttgattttca tatgcatccc gactcgccac gaccaactca tcactctgct agcggcccag
     3421 tctcacctca ggatcgaacc tcttcacgca gcgcatccgg atcgacgatg acgatgtacc
     3481 cctgcaccgt gttaaccagc cctctgcgcc ggaatgcccc catcgccttg ttgatgctct
     3541 cgcgcgtcgc gccgatcagg ctcgccaggt cgctctgcgt caggggcgag ttgatgcgca
     3601 cgccgccctc ttcggcgacg ccgtactcgt gcgccaactg cagcagcttg cgcgccagcc
     3661 gggccggcac gtcgaggaac gtcagcgtct cgacctgctg cgtgctgtaa cgcaggcgcg
     3721 cgctgagcgc cttcaagaag ttgagcgcca actgcggctc gcgccgcatg tgctcgcgca
     3781 actggtcgcg ggtgagcgtc agcacggcgg tgtcctccat cgccgcgacg ctcgccgagc
     3841 gcggcatctc gtcgatcacg gccagctcgc caaatatatc gcccgggccg tacaagatga
     3901 ccgaggtctc ctgcccctct tcgcccacga cgaagacgcg aacgcggcct gaccggatca
     3961 ggtacagcaa ctggcccgcg tcgccctgca gaaagatcgt ctcgccctgg cgaaactgcc
     4021 gggtgacgaa atcgcgggcg acagactcca gctccgtatc gctcaggccc gcaaacagcg
     4081 gcgcctgcct caggaattcc aaaatcgccg tcactcgagt gcctgccttg agattcgcat
     4141 cgcgctggca ggtattgtat tcccgcagcc acgtactcgc aaggatcaga gtgttttgtg
     4201 aagtcgacgc catcgccgcc tccctgatta caccaggcgc agccccagac cgaccgccac
     4261 ggcgagcgcg acactcgcca gcagctcggc gacgtacacc gtcgcccgcg ggctacctgc
     4321 cacctgccgc cactccatcg ccagcctcgg cagggcgacg aggggcacca gagcgatctg
     4381 ccccagcagg accgacacag tcatcagcgc gcgctccaga ctgctgacgt acttgttcgg
     4441 cgcctgcgag aaatcggggc cggacccctt gagcaagccg tacacgacga actcgaccag
     4501 cacccaggcc ggcgcggtca caaacaccag gccgatggcg aataccagca ggcgatccgt
     4561 gagcagcacg ggcgccagat cggccagccg tgccggggcg gccagatagc cggactcaac
     4621 cagcgcaaac gtaatgacgc tcaagtgcac agcctgatcg agcacgaaca gcgccagcgc
     4681 cgagaatgcc tttcccagcc gcagccggat cgcgtcgacg gcggtgtgcg tcagccagac
     4741 gaacagcacc caaggccacc atccagcgtc gaacggcagc gtgcaggccc acgtcactgc
     4801 cagcacgacc aggccgtgaa cgactacgcc cttgtactcc cggctcttcc acaatgccaa
     4861 ccgatcccac tgcaagacgt aatccgcaat caggtgtgag agatacatcg ccagaaccat
     4921 agccaactcc gtcgcgtcga atccgcactc aggatagcct atctcgcatc gggccgctat
     4981 gaaatcggtc acaggccgtg cttgatccag ggtgcggatt ctagcgcgta aaatagacac
     5041 tcctggaggg gctttaggct caagccgccg tccccggctc ggctaacgcc gccggggacg
     5101 gcgcggcgag tcgaatctga catcgtcacg atagacacgc ccggaggggt tgatggaact
     5161 ggcactctgg actgttgctc tctgcctgct ggtgacccat atccggccct tgacatgggc
     5221 gcgcaaggcg accgggcgag cgcagcgaag catccgcgtg gcgagtgtcg ggctgctgct
     5281 gattctcgcc gtgctcctcg cggcgagcgg cgcgtattcg ttctggattg cgcaccggcc
     5341 cttgcccggc aacacgcgcc aggtcgtctt cgaaggaatc acctacatcc gcgaagtgcg
     5401 ccgctccccg cggccgatcg tgattcacgt catgcgcgtc gagctggatg cgcccggcat
     5461 ccgcttcctg gtcacgccgg gtgacccatc gccggaagac gagattcgag cgcgcacgac
     5521 ttcgcaattc gtggcggaat ttgatctgca ggtggcgatc aatggcgact tcttcacgcc
     5581 atggtggtcg tacggcccat gggattacta cccgcgcgtc ggcgatccaa tcgacgtgaa
     5641 gggcttcgcc agctcgaacg gagcggttta ctccggcggg gagtcgaatg acgggccgac
     5701 gctttatctc tcagccggca accaggccag cttcgacgtg ccgatcggga gcgtgtacaa
     5761 cgccatctcc ggcaacacga tcttcctgga agatggcgtc gcacgggtag aaccaccgga
     5821 gtatcacttt gagccgcatc cgcgaaccgc catcgcactg gatcgcgcgc gtcgcacact
     5881 cattctcgtc gtcgtcgacg ggaggcagcc caactacagc gagggcattt cgctggccga
     5941 gcttgcgcaa atcctgcgcg attatggtgg agacacggcg ctcaacctcg acggcggcgg
     6001 ctcgacgacg atggtgatcg agggggacaa cggagagccg gtcatcctga attcgccgat
     6061 cgacaatctt atcccgggcc gcgagcggcc ggtcgcgaat cacctgggca tttacgccca
     6121 tcctgcatcc gcgccgtgac ttttatcagg catggccaga cccggaagag gtggcattat
     6181 ccccaggcaa gacaaaagac accgggctaa accagcctcg gtgtcccgtc ttagacatga
     6241 cccaggaggg acttgaaccc ccaaccgagc ggttaagagc cgcctgctct gccaaattga
     6301 gctactgggc caaacttcgc tgctgataag agttcactgc tctgccgaat tgagctagcg
     6361 gcccgtgtga catgccagat tataggtttc cgttctctca ttgtcaagtt cgatcacctg
     6421 ttcacacgat cacgtaatca cgtaatcacg tagtcacgtc atcacgcacc acgcaacacg
     6481 caacacgcat cacgcatcac gcaacacgca ccctcatttg acacccacgc ccactcagtg
     6541 tacgctatat atcagcacac ctgttcggga gaggtcatgt cagcagagca gctttcattg
     6601 ttcgacgggt cgagccagcc tgagccggcg cccgccgagg gcgaaacggg agtggacctg
     6661 tcggtggcga gggcgctcac gtcgcgcacg cgcctctcgg tcgccatgga agcttatcgc
     6721 gatcacatga tcgcccgcca gttcagcgag aatacggtca aggcgttcat gtacgatctc
     6781 cacctcgtcg cggagttctt cacgccgatt aagccgatcg gcgaggttgc gcatcgcgac
     6841 ctggagcggt tcgtcgactg gctggtgacc gggcgcggtg tgccgtgcaa gccgaagtcg
     6901 ctggcgcggc gcatcacgac gctcaaggtg ttcttcggct ggctgcacga gatcggcgcc
     6961 atcccagtcg atccggccgc gccgatcgtc caccggcccg ttacgacgcc cttgcccgac
     7021 atcctgtttg acaatgagat cgacgcagtg ctggcggcga cgcagcggct gcgcgccggc
     7081 gtcgcgagcg aacccggcgc cccacctaga cctgacgcgc gcccccacct gctggtgact
     7141 cttctgctaa gcaccggcat caagaagagc gagtgcgtcg cgatcaagct cgagcacatc
     7201 gacctgtccg atgcaggcga cccggcgctg tggatacgct acgccgacgc gcgccaccgg
     7261 cacaaggagc gcaagctgcg tctcgatcct cgctggccca aggtgctcga cgagtataga
     7321 acccaatacg acgtcaagga attgatcttc ccctggtcgg cgcgcaatct cgaatacgtg
     7381 ctgaccgacg tcgggaagat ggcgggcctg accaagccac tgtcattcga gacgctgcgc
     7441 tggacgtgcg ccgtgaccga caagcgcaag ggcatgcccg acgagacgct gcgccgcaag
     7501 atgggtctgt cgcagatctc ctggcgtgaa gtaggcgaaa agctggtcaa gctggctgct
     7561 ccggcgttgt agtggccacg taatcacgtt atcacgttat cacgcatcac tcgtcacgcc
     7621 ttcaaccaac acccacaacc ccgccgttac cagtgaatgg ccgcctagca gaatcgggat
     7681 cgggcacccg gcggctgcca gcgtgaactc tcgtaagaac ggatcgtcga tcacttcggg
     7741 catgagcagg tcagacgcaa accgcgcggc atcgtccggc cagatgttgt gcg
//
LOCUS       DBNHXB010000315         5668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_421587, whole genome shotgun sequence.
ACCESSION   DBNHXB010000315 DBNHXB010000000
VERSION     DBNHXB010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_421587"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="AC162G_12695"
     CDS             <1..771
                     /locus_tag="AC162G_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5076722.1"
                     /translation="LSHAAGHDINYIALSGALSLIGRSGDRPVAPPAMIGDFGGGGTF
                     LAFGIVCALLEARTSGQGQVVDVAITEGSALLTALIHGLKAMHVWNDARGTNVLDSGA
                     HFYDTYECADGKWVAIGALEPQFYALLLEKCGIHDPEFSVQMDKRRWPALKEKMAALF
                     RSKTREEWRMLLEGTDACFAPVLDLDEAPQHPHNQARQAFVEVGGVQQPAPAPRFSRT
                     PPDTPSPPSLPGEHTGEVLAEWGFSAEEIAQLKAGGII"
     gene            959..2947
                     /locus_tag="AC162G_12700"
     CDS             959..2947
                     /locus_tag="AC162G_12700"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5076723.1"
                     /translation="MSKKKPRSGKKPGPSTPRPLSAKLVDALEEADSLMQRGRLDEAI
                     DVLESADRRYPNRPEVLTQLMNVYHDVEDMIGYMSVAERLSKLVPNDPDLTLALAGAY
                     AANLFPALALRTFHHFLKRWPDHAHADEVRKMAPDLESFLRAEQDELDMPGEAGLELL
                     TMHDTVRSCLAQGRYREARQAAEDLLRRRPDFAPALNNMSQAYYAEGKIDRAISTTQR
                     VLAFEPDNVHALSNLTRYLCLSGHPDEARQSAERLKASAAQAADVWLKKMEALTVLGD
                     DQGVLDVFHHAEQAGALQSPDAATLYHLAAVAALRLTREEDAREYWKRALKLSPDLEL
                     ARDNLDDLRKPARERHAPWAFGAGNWITPQAIQDLIVHIEPASRRADDDAVTHAARRY
                     LRKHPEMNGLAPVLLDRGDPDARGFALRLALMAETPEMLAVLRDFALSPRGPDEMRFQ
                     AAQAAATAGLLPPGPARLWQDGEWRELLLLGFEIHGEPLTQHQPPVEQWALDAMQALR
                     DSDFIEAERLLKQALEIQPDQPDLLNNLAAAYDRQGRAVESQALLRQVHEQYPDYLFA
                     RTGLARLHVQNGQLAEARALLEPLWTKKRWHFSEYASFCGAQIELLLAEGNREAAQSW
                     LDMWADANPDHPDLHRYRDLLGRTGRLRHLFKRPARPK"
     gene            complement(3004..3525)
                     /locus_tag="AC162G_12705"
     CDS             complement(3004..3525)
                     /locus_tag="AC162G_12705"
                     /inference="COORDINATES: protein motif:HMM:NF025637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR5076724.1"
                     /translation="MVNVLLLNASYEPLHIIGLPRAVNLLLVGKAEMVEAAPGREVRS
                     PSRRQPMPSVLRLRYYVNVPQRGAAWSRRGVLIRDRYTCRYCGRKLQARQATIDHIIP
                     QWKCRRQGIPANTWGNTVASCWRCQQRKAGRSMHEAGMRFHNPGFEPKTPRTIYLVMT
                     SDIRPEWRQYIRL"
     gene            complement(3538..3611)
                     /locus_tag="AC162G_12710"
     tRNA            complement(3538..3611)
                     /locus_tag="AC162G_12710"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3575..3577),aa:Ile,seq:tat)
     gene            complement(3618..3691)
                     /locus_tag="AC162G_12715"
     tRNA            complement(3618..3691)
                     /locus_tag="AC162G_12715"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3656..3658),aa:Phe,seq:gaa)
     gene            complement(3751..3824)
                     /locus_tag="AC162G_12720"
     tRNA            complement(3751..3824)
                     /locus_tag="AC162G_12720"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3788..3790),aa:Leu,seq:cag)
     gene            complement(3830..3901)
                     /locus_tag="AC162G_12725"
     tRNA            complement(3830..3901)
                     /locus_tag="AC162G_12725"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3866..3868),aa:Met,seq:cat)
     gene            complement(3961..4035)
                     /locus_tag="AC162G_12730"
     tRNA            complement(3961..4035)
                     /locus_tag="AC162G_12730"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4000..4002),aa:Asn,seq:gtt)
     gene            complement(4042..4114)
                     /locus_tag="AC162G_12735"
     tRNA            complement(4042..4114)
                     /locus_tag="AC162G_12735"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4079..4081),aa:Arg,seq:tcg)
     gene            complement(4117..4189)
                     /locus_tag="AC162G_12740"
     tRNA            complement(4117..4189)
                     /locus_tag="AC162G_12740"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4154..4156),aa:Pro,seq:tgg)
     gene            complement(4261..4345)
                     /locus_tag="AC162G_12745"
     tRNA            complement(4261..4345)
                     /locus_tag="AC162G_12745"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4308..4310),aa:Ser,seq:cga)
     gene            complement(4354..4427)
                     /locus_tag="AC162G_12750"
     tRNA            complement(4354..4427)
                     /locus_tag="AC162G_12750"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4391..4393),aa:His,seq:gtg)
     gene            complement(4445..4517)
                     /locus_tag="AC162G_12755"
     tRNA            complement(4445..4517)
                     /locus_tag="AC162G_12755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4482..4484),aa:Arg,seq:tct)
     gene            complement(4521..4592)
                     /locus_tag="AC162G_12760"
     tRNA            complement(4521..4592)
                     /locus_tag="AC162G_12760"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4557..4559),aa:Trp,seq:cca)
     gene            complement(4781..4855)
                     /locus_tag="AC162G_12765"
     tRNA            complement(4781..4855)
                     /locus_tag="AC162G_12765"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4817..4819),aa:Ala,seq:tgc)
     gene            complement(4968..5039)
                     /locus_tag="AC162G_12770"
     tRNA            complement(4968..5039)
                     /locus_tag="AC162G_12770"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5004..5006),aa:Thr,seq:tgt)
     gene            complement(5386..>5668)
                     /locus_tag="AC162G_12775"
     CDS             complement(5386..>5668)
                     /locus_tag="AC162G_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIR5076725.1"
                     /translation="FRRLARRSAETRQAATSQRDAVILDMLADGWKIPTIIQLTFDDL
                     PELRKYLRNGLGHYLQSERPETDSPNLFVSKSGRPLTLANTYKVMQHKK"
BASE COUNT         1065 a         1862 c         1719 g         1022 t
ORIGIN      
        1 ctgtcccacg cggccggcca cgacatcaac tacatcgcgc tatcgggcgc gctgtctctc
       61 atcggccgga gcggcgacag accggtcgcg ccgccggcga tgatcggcga tttcggcggc
      121 ggcgggacgt tcctggcctt cggcatcgtc tgcgcgctgc tggaagctcg cacatcgggg
      181 caggggcagg ttgtggacgt ggcgatcacc gagggctcgg ccctcctgac ggcgctgatc
      241 cacggcctga aagccatgca cgtgtggaac gacgcgcgcg ggacaaacgt gttggacagc
      301 ggcgctcatt tctacgacac ttacgaatgc gcggacggca agtgggttgc gatcggcgcg
      361 ctcgagccgc agttctacgc gctgctgctt gagaagtgcg gcattcacga tccggaattc
      421 tccgtccaga tggataagcg tcgttggccg gcactgaagg agaagatggc ggcgctgttc
      481 cgctcaaaga ctcgcgagga gtggcgcatg ctgctagaag gaacggacgc ctgtttcgcg
      541 ccggtgctcg acctggacga agcgccgcag catccacaca atcaggcccg ccaggctttc
      601 gtcgaggtcg gcggtgtgca gcaacccgca cccgcgccgc gcttcagccg tactccaccg
      661 gacacgccgt cgcctccgtc gctccccggc gagcataccg gcgaggtgct ggccgagtgg
      721 ggcttcagcg ccgaggaaat cgcccagttg aaagcaggcg gcatcatcta ggttgcacag
      781 tgtgcacgca aaacatgcca ggccggacgt agtgcggctt tccatagccg ccaatttgcg
      841 gcgcgtatgg aaacgcgccc tacggttgat gacaactttt gcgagcacat gttgcacaat
      901 ttccgtttga tctctgagcc gattgccggt agaatagatc gcggtccctc acaagcctat
      961 gtccaaaaag aagcctcgat cgggcaagaa gcccggccct tcgacacccc gtcctctctc
     1021 tgcgaagctc gtcgacgcgc tggaggaagc ggattcgctt atgcagcgag gacggctcga
     1081 cgaggcgatc gacgtcctcg aatcggctga ccggcgttac cccaatcggc cagaagtgtt
     1141 gacccaactg atgaacgtct atcacgacgt cgaagacatg attggttaca tgtccgtcgc
     1201 cgaacgcctg agcaagcttg tgcccaacga tcccgacctc acgcttgccc tcgccggggc
     1261 atacgcggcc aacctgtttc cggcgctggc cctgcgaaca ttccaccatt ttctcaaacg
     1321 ctggcccgat cacgcgcacg ccgatgaagt gcgcaagatg gcacccgatc tagagtcgtt
     1381 tttgcgcgca gagcaagatg aattggacat gcccggcgaa gcagggcttg agttgctgac
     1441 gatgcatgac acggtgcggt cctgcctggc gcagggcagg tatcgagaag cccgccaggc
     1501 tgccgaagac ctgctgcgtc gccgccccga tttcgcgccc gcgctgaaca acatgagcca
     1561 ggcatactat gccgaaggca agatcgatcg agcgatctcc acgacgcagc gcgtgctcgc
     1621 cttcgaaccg gacaacgtcc acgccctgtc taacctcacg cgttacctgt gcctgagcgg
     1681 ccatccggac gaagccaggc agtcggccga acgcctcaag gctagcgcgg cccaagcggc
     1741 ggacgtgtgg ctcaagaaga tggaagcgct caccgtcctc ggcgacgacc agggtgtact
     1801 ggatgtcttc catcatgccg agcaggccgg cgcgctccaa tcgcccgatg ccgcaacgct
     1861 ctatcacctg gccgccgtcg ccgctttgcg tctgacgcgc gaagaggatg cacgcgaata
     1921 ctggaaacgc gcgctgaagc tatctccaga tttggagctg gcgcgcgaca acctggacga
     1981 cctgcgcaaa cccgcccgcg agcggcatgc gccgtgggct ttcggagcag gcaactggat
     2041 cacaccgcag gccatccagg acctcatcgt tcacatcgag ccggcatccc gccgggccga
     2101 cgacgacgcg gtcacgcatg ccgcacggcg ctacctgcgc aagcatccgg agatgaacgg
     2161 attggcgcct gtcctgctcg atcgaggtga cccggacgcg cgcggcttcg cgctgcggct
     2221 ggcgctgatg gctgaaacgc ccgaaatgct ggccgtgctg cgcgacttcg cactcagccc
     2281 acgcgggccg gatgagatgc gcttccaggc ggcgcaggcc gccgccactg ccggattgtt
     2341 gccccccggg ccggcacgct tgtggcagga tggcgagtgg cgcgagttat tgctattggg
     2401 cttcgagata catggcgagc cgctcacaca gcaccagccg ccagtcgagc aatgggccct
     2461 cgacgctatg caggcgctgc gcgacagcga ttttatcgag gctgagcgcc tgctcaagca
     2521 ggcgttggag atccagccgg accagcccga ccttctcaac aacctggctg cagcgtacga
     2581 tcggcagggg cgcgcggtcg aatctcaagc cctgcttcgg caagttcacg agcagtatcc
     2641 cgactatcta ttcgcccgca cgggtcttgc caggttgcac gtacagaatg ggcagcttgc
     2701 ggaagccaga gcgctccttg agccattatg gacgaagaag cgctggcact tcagcgaata
     2761 tgcgtcgttc tgcggcgcgc aaatcgagct gttgcttgca gaaggcaatc gagaagccgc
     2821 tcagtcctgg ctggatatgt gggcggatgc caatccggat catccggatc tgcacaggta
     2881 ccgtgacctt ttgggccgga caggccgatt gaggcatctc ttcaagcggc ccgcgcggcc
     2941 caagtaagga ccgcatcccg caccccccgc ccgagtgagc agggtggggg tgttgtcatc
     3001 tgtctacagg cggatatact gccgccactc cggccggatg tccgaagtca tcaccaggta
     3061 gatcgtgcgc ggcgtcttcg gctcgaagcc tgggttgtga aagcgcatgc ccgcttcgtg
     3121 catcgatcgg cctgccttgc gctgctggca gcgccagcag ctcgccaccg tgttgcccca
     3181 ggtattcgcc gggatgcctt gccgccggca tttccactgc gggatgatgt gatcgatcgt
     3241 cgcctggcgc gcttgcagct tccggccgca gtatcggcag gtgtagcgat cgcggatcag
     3301 cacgccgcgt cgcgaccagg cagcgccgcg ctgcgggacg ttgacgtagt atcgcaggcg
     3361 caggacgctg ggcatcggct ggcggcggct cggcgaacgc acctcgcgcc cgggcgccgc
     3421 ctcgaccatc tccgccttgc ccaccagcag caagttcacc gcgcgcggga ggccgatgat
     3481 gtgcagcggc tcgtaactgg cgttcagcag caatacgttg accatgtcaa atccttatag
     3541 gcctgcaggg agtcgaaccc tctctgactg ccttataaga gcagcgccct ctaccggaga
     3601 gcaccaggcc cacacagtgc cgcccgtcgg actcgaaccg accccttccc ggcgcttcag
     3661 accggagccc taccggctag gccagagcgg cagtgacggc ctcggggaga ctcgaactct
     3721 caacattcag attcgcatcc tgtcgcccag taccagccct cggactcgaa ccgagatgaa
     3781 ccgcggtctg agcgcggtgc gtctgccatt tgcgccaggc tggcacagat agcgggtacg
     3841 ggaatcgaac ccgcctcatc ggcttatgag gccggcctcg tcaccagacg agtcacccgc
     3901 aatgctcccc tctcccgcag tgcgcctctc cactgcggga gaggggtcgg gggtgaggga
     3961 ctgatccggc gggattcgaa cccgcaccgc gcctcgatta acagtcgagt gccctaccgg
     4021 ttgggcgacg gatcaatgca gaaccctcgg tcggatttga accggctgtc tccgcgttcg
     4081 aagcgcggcg tgcgtcctat cgcaccgcga gggcagtcgg gaaggcggga tttgaacccg
     4141 cgacctctgc agtccaagtg cagtacgctg ccaactgcgc cacttcccga tgcacccctc
     4201 accggttgca cagccggtga ggggtcgtct tgcgccaaca cccgtggcca tcagacgagg
     4261 aggagacggc gcgagtcgaa cgcgcagccc tcgtcgggcg cccgttttcg agacggggtg
     4321 gcactcccac tatgccgacg tctccaccgc aggggtgacc ggcgagactc gaactcgctt
     4381 ccacggcggc cacaacgccg cgcctcgacc tcttcggcat cggtcaccat atgatcaggc
     4441 ctcacaccct cggcgggact cgaacccgca gcctgctgtt tagaagacag ccgctcgtcc
     4501 gatcgagcca cgagggcagg caggtcggcc aggactcgaa cctgggttgc atcttttgga
     4561 gagatgccga tgggccacca tcgtcccgac cctctgctcc agacctcacc ggtctttgag
     4621 acctgttagg tcttctgagt tcagtgcgga tggggccgac ttctcccgcc gctacctgcg
     4681 aagaccaggc gttccccgcg ccacgtgcat gttctcccgg ctcttcaccg ggtacgcggc
     4741 cgcgttcgag cggcgaccat cgaagatcca ttccgcacag tggacccggc gggctttgaa
     4801 cccgcttctc taccgtgcaa aggtagcgag gagcccgact cctccccaca ggcccgaaca
     4861 atctccactc tccaatctct aatccaccac tcccatccgc tgccggctcg cacgccgcgc
     4921 gccgaacgtg caagccggca ccgccaggaa acgcgcgttt ccttcagagc ctggacgtgg
     4981 acttgaacca cgaccatcgc tttacaagag cgccgcactc ccgattatgc aatccaggca
     5041 acgcatcccc acaaaacaac ccggcgcgaa ggttacgcgc ctccgcgccg ggctggctta
     5101 cagaaataaa aagcgcgaag gtcgtagcta tccttcgcgc ctgctgagcg taaacaccgg
     5161 aagcctagct tccccctccc tcaacaggcg catagccaac cgaatccgta ccggaggcga
     5221 actgccacgg cgctgattcg agcaggatgt gtctacgact gtcggatgtc gatattccta
     5281 acatggcgtc acactcattt tgttgcttag ccgctgatat tatacgctcg attctgggtt
     5341 tgtcaagatg caattttgtc tctttacgcc gatccgggac aaatttcact tcttgtgctg
     5401 catcactttg taggtgtttg cgagtgtcag aggccggccg gatttcgaga cgaacaggtt
     5461 tggcgaatcg gtttcagggc gttcggattg caaatagtgt cccagtccat tcctcaggta
     5521 cttcctcaat tcgggtaagt cgtcaaaggt cagttgaatg atcgtcggta ttttccagcc
     5581 atcggctaac atgtccagga tcaccgcatc gcgctgcgaa gtggccgcct gcctcgtctc
     5641 cgccgagcgc cgggcgagtc tgcgaaag
//
LOCUS       DBNHXB010000316         5360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3273258, whole genome shotgun sequence.
ACCESSION   DBNHXB010000316 DBNHXB010000000
VERSION     DBNHXB010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3273258"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..718)
                     /locus_tag="AC162G_12780"
     CDS             complement(89..718)
                     /locus_tag="AC162G_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR5076726.1"
                     /translation="MTSSSSSNPPETLTDRARQFFARFLTPIVDVLIRLGVTPNMVTA
                     FGFVANVVVGALAAAGYLTAAGIALLLSGAMDGLDGALARRLGKASKFGAFLDSTLDR
                     YSEAVVLFGVLVYATPRGLQTEQMLVTITVVGSLMVSYTRARAESLGVDCKIGLLTRL
                     ERVVILSATLVFQQVTIGLLLLGVLTHITAFQRMIVVLRTLRRVEGASR"
     gene            complement(782..1870)
                     /locus_tag="AC162G_12785"
     CDS             complement(782..1870)
                     /locus_tag="AC162G_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HIR5076727.1"
                     /translation="MARKASSKKVRVAIIGVGNCASSLVQGVEYYRSAPDDAQIPGLM
                     HVRLGGYHIHDVEFTCGIDIDKNKVGKDLCEAIFIAPNNTYKFSDVPHLNAPVYRGMT
                     HDGLGKYLSKIVEKAPGPTDDIVQILRDTKTDVVVSYLPVGSEEATKWYVEQILKAGC
                     AFVNCIPVFIAREKYWQKRFEDAGLPVIGDDIKSQVGATITHRVLTRLFEDRGVRLDR
                     TYQLNFGGNTDFLNMLERERLESKKISKTNAVTSQLSRELPEKDVHVGPSDYVPWLSD
                     RKWCYIRMEGTSFGDVPLNLELKLEVWDSPNSAGVVIDAVRCAKLALERGLKGTLVAP
                     SSYFMKSPPRQYTDDKARQMVEDFIAGK"
     gene            complement(1951..2265)
                     /locus_tag="AC162G_12790"
     CDS             complement(1951..2265)
                     /locus_tag="AC162G_12790"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5076728.1"
                     /translation="MTARSADRAEAKALRDLARHFYALKDAMRLEMIALMADRDECTV
                     YEMAAALNKSQPLVSWHLRRLKAAGIVKMRRSGRKVYCSLDRDSIARHQEEFRRLIQR
                     AP"
     gene            complement(2442..3341)
                     /locus_tag="AC162G_12795"
     CDS             complement(2442..3341)
                     /locus_tag="AC162G_12795"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR5076729.1"
                     /translation="MSMASLALLLAAAGFHAAANALIKQSRDKLAFTWWMLSVTSILG
                     LPFLLVVGDAQPVGWAIVLLSGLLEAVYFVALTQAYSHGDLSQVYPIARGSAPLFVAL
                     WAAVFLGERPSPGGVLGILLVVAGLYLVNLPSLSAWKQPLQGFKNQAARYALLTGLLI
                     SIYSTVDKVGVRYFDPVIYLYLILLAGWIFLTPIWLQPGRRAALMAEVAPEPASEAAG
                     QNRVRRAARVAACALLGTAAYLFVLTALRISPVSYVSPVREMSVVIGAWIGVRFMGEP
                     GGAMRIVASLLVALGVLTIAVAG"
     gene            3478..3551
                     /locus_tag="AC162G_12800"
     tRNA            3478..3551
                     /locus_tag="AC162G_12800"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3512..3514,aa:Pro,seq:cgg)
     gene            3798..3947
                     /locus_tag="AC162G_12805"
     CDS             3798..3947
                     /locus_tag="AC162G_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076730.1"
                     /translation="MGVVVLTMGEEEAMALNRDGSFYRARTEPAPEIDPLGSGNARTA
                     GQITG"
     gene            complement(4046..4612)
                     /locus_tag="AC162G_12810"
     CDS             complement(4046..4612)
                     /locus_tag="AC162G_12810"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR5076731.1"
                     /translation="MPRSDNLYEIPDNVPVPVDDGACNHLTNMRLPSVALRSTSGHLV
                     DLASLAGRVVIYCYPRTGRPDMDPPQGWNEIPGARGCTPQACAFRDHYQELQKLGAQV
                     FGLSTQDTDYQREAVARLHLPFELLSDSGLGFANRLRLPIFEIAGMKLIKRLTLIARD
                     GQIEKVFYPVFPPDKNANEVVNWLARHK"
     gene            complement(4646..5293)
                     /locus_tag="AC162G_12815"
     CDS             complement(4646..5293)
                     /locus_tag="AC162G_12815"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5076732.1"
                     /translation="MRAVVFDLDGTLVNTTGLILNTYVETIRSLGRGDVTTYDILSNF
                     NIGPTPVILERFLGRPILPQDLDAYYVAYEAAISRLQPFPRVAGMLKQLHRKGYRLGL
                     FTSATRRAVNLVLPRTGLDGHFGAIVAGDEVTHSKPAPDGLEIVCRRLGVRTEEAAYV
                     GDAEVDVACARNARVLGVQAVWGSASVIFAGDHLVANQPDEIIALIESASHAVLK"
BASE COUNT         1077 a         1682 c         1597 g         1004 t
ORIGIN      
        1 cgatcatcgc gccgccgtca catggaccgg tcagtgatta tatcagggcc gtttgcccga
       61 gacaatgctt ctgaggtacg ggatgatgtt acctcgacgc tccctcgacc cggcgcagcg
      121 tccgcagcac gactatcata cgctgaaagg ccgtgatatg ggtcagaacg cccagcaaca
      181 gtaacccaat tgtaacctgc tggaagacaa gcgtggccga caggatgacg acgcgctcca
      241 gccgcgtgag caggccgatc ttgcagtcga cgcccagcga ttcggcgcgc gctcgcgtgt
      301 aactgaccat cagagagcct acgaccgtga tcgtcaccag catctgctcg gtctgaaggc
      361 cgcgcggggt cgcgtacacg agcacaccga acagcacgac cgcctcggaa tagcggtcga
      421 gcgtcgagtc gaggaacgcg ccgaacttgc tggccttgcc cagacgccgc gccagcgcgc
      481 cgtcaagacc atccatcgcg ccggatagca gcagcgcgat gccggcggcc gtcagatagc
      541 cggcagccgc cagggcgccg acgacgacgt tcgccacaaa accgaaggcc gtcaccatgt
      601 tcggcgtgac gcccaggcgg atgagcacgt ccacaatcgg tgtgaggaac cgggcgaaga
      661 actggcgcgc gcggtcggtc agcgtctcgg gtggattaga cgatgaggat gaagtcatgg
      721 acgcaagccc cggctatgcc tgcggcgcac tggcaaggtg cgtcgcaggc acacgtcaat
      781 tctacttacc tgcgatgaag tcttcgacca tctggcgcgc cttgtcgtcg gtgtactgcc
      841 tgggcggcga cttcatgaag taagacgatg gcgcgaccag tgtgcccttc aagccgcgct
      901 ccagcgccag cttggcgcag cgcaccgcgt cgatgacgac gcccgccgaa ttggggctat
      961 cccacacttc cagcttcagc tcgaggttaa gcggcacatc gccgaaggac gtgccttcca
     1021 tgcggatata gcaccacttg cggtcgctga gccacggcac gtagtcgctc gggccgacgt
     1081 gcacatcctt ctccggcagc tcgcgcgaca actgactggt gacggcgttc gtcttgctga
     1141 tcttcttcga ctcgagccgc tcgcgctcga gcatgttcag gaagtcggtg ttgccgccga
     1201 agttcaactg gtaagtgcga tcgagccgca cgccgcggtc ctcgaacagc cgcgtcagca
     1261 cgcggtgcgt gatcgtcgcg ccgacctggc tcttgatgtc gtcaccgatc accggcaggc
     1321 ccgcatcttc gaagcgcttc tgccagtact tctcgcgcgc aatgaagaca gggatgcagt
     1381 tgacgaatgc gcagccggcc ttgaggatct gctcgacgta ccacttggtc gcctcttcgc
     1441 tgccgaccgg caggtaggat acgacgacgt cggtcttggt gtccctaaga atctggacga
     1501 tgtcgtccgt cgggccgggc gctttctcga cgatcttcga caggtatttg cccagcccgt
     1561 cgtgcgtcat gccccggtag accggcgcgt tcaagtgcgg gacgtcggag aacttgtacg
     1621 tgttgttcgg cgcgatgaag atcgcctcgc acaggtcctt gcccaccttg ttcttgtcga
     1681 tgtcaatgcc gcatgtgaat tcgacgtcgt ggatgtggta accacccagt cgcacgtgca
     1741 tcaaaccggg gatctgagcg tcgtcgggcg cgcttctgta atactcgacg ccctgcacca
     1801 acgacgaggc gcagttaccc acgccgatga tcgctacgcg cactttcttg ctggaagcct
     1861 tccttgccat gaatggactc cttgaagagg taattggata ttggatgttt gaattttgac
     1921 ctttgagctt tggaggttgg atcgattctt tcatggcgct ctttgaatca gtctccgaaa
     1981 ctcctcctga tgccgggcta tggaatctcg gtcgagcgag caatacactt tccggcctga
     2041 tcgccgcatc ttgacgatac cggccgcctt caagcggcgc aggtgccacg acaccagcgg
     2101 ctggcttttg ttcaacgccg cagccatctc atagaccgtg cactcgtccc gatcggccat
     2161 cagcgcgatc atctccagcc gcatcgcgtc tttcagcgcg tagaaatgcc gggccaggtc
     2221 gcgcagcgcc ttcgcctcgg cgcggtcagc cgatcgagcc gtcatggcaa agcctcaaat
     2281 tcataaacaa acgcttatat attatcagga ctcgccccgg gtgtcaagtg agccgttcag
     2341 cccacacgca agacatgtca gcggaggcgg ctcccacgcc gccgcataag ccgggcgtgg
     2401 gagctcggcc tgctttcctg acagcgctcg tgtgcacatt ctcaacctgc cacggcaatc
     2461 gtcagcacgc cgagcgcgac caggagcgag gcgacgatgc gcatcgcccc tcccggctcg
     2521 cccatgaacc gcacgccgat ccaggcgccg atcaccacgc tcatctcgcg caccgggctg
     2581 acgtagctca ccgggctgat gcgcagcgcg gtgaggacga acaggtaggc cgccgtgccc
     2641 agcagcgcgc atgccgccac ccttgcggca cggcgcacgc gattttgccc ggcggcttcg
     2701 ctggccggct cgggcgcgac ctcggccatc agcgcggcgc gccggcccgg ctgcagccag
     2761 atcggcgtca aaaagatcca tccggcgagc agtatcaggt aaagataaat cacaggatcg
     2821 aaatagcgca cgccgacttt gtccaccgtc gagtagatcg agatcagcag ccccgtcagc
     2881 agcgcgtagc gcgcggcctg gttcttgaat ccctgcagcg gttgtttcca cgccgacagc
     2941 gacggcaggt tcaccagata cagtcctgcc acgaccagga gaatgcccag cacgccgccg
     3001 ggcgatggcc gctcgccgag aaagacggcc gcccagagcg cgacgaacag tggagcggag
     3061 ccgcgcgcga tcggatacac ctgcgacagg tctccatgag aataggcctg cgtcagcgcc
     3121 acgaagtaga cggcctcgag cagcccgctg agcaagacga tcgcccaacc caccggctgc
     3181 gcatcgccga cgacaagcag aaagggcagg ccgagaatgc tggtcacgct cagcatccac
     3241 cacgtaaaag cgagcttatc tcgactctgt ttgatcagcg cgttcgcggc ggcatggaat
     3301 cccgccgcgg cgagcagaag cgcgagactc gccattgaca tgagtgatta cacccgagtc
     3361 cgtttatagc gcaagcaccc gcggccggcg gcgcggcacg cgagtgcttg cacggcgaac
     3421 aggaatcgat tataatgcac atcggtcgcc ggcctgaacc tacagcgact gtaagttcgg
     3481 ggtgtagcgc agaccggtag cgcgcacggt tcgggtccgt gaggtcccgg gttcaagtcc
     3541 cggcaccccg actcgttgtg accaaagacc gcgtggatcg aggacttctc tatccgcgcg
     3601 gtttgcgttg atctgctcgc cacgaaacgg tttatccctc gcccgcgccc gcgcctgacc
     3661 gcacgcctca acctgtcgca gatacagccc ctgacgctcg ttgccgcgcc ggccggcttc
     3721 ggcaagacgc gtatcggctg ttcggctgcg ccgctggcgc ggaagagaca ctgcgttggc
     3781 tccaggccaa gttcaacatg ggggtggttg tgctcacgat gggcgaagaa gaagcgatgg
     3841 ccctgaatcg agacggctct ttctaccgcg cccggacgga gccggcgccc gagatcgatc
     3901 ccctgggcag cggcaacgcc cgtactgcag ggcaaatcac gggctgagtg gggcaatatg
     3961 tgctacgcgc tctactgccc aagccgaacg cggcagggca aagccgatgc gcttaaagcc
     4021 attgtggggc aacttgctga ttcggttact tatgccgtgc taaccagtta accacttcat
     4081 ttgcgttctt atcaggcgga aagaccgggt agaaaacttt ctctatttgg ccgtcgcggg
     4141 caatcagagt taatctcttg atgagcttca tccctgctat ttcaaagatg ggcagtcgca
     4201 accggtttgc gaatccaagc ccggagtcgc tgagcagttc aaacggtaaa tgcaatcgtg
     4261 caacggcctc ccgttgatag tcagtatctt gggtgcttag gccgaacacc tgtgcgccaa
     4321 gcttttgtaa ttcttggtaa tgatctcgaa aagcacaagc ctgcggtgta cagcctctcg
     4381 ccccggggat ttcattccaa ccctgaggtg ggtccatgtc cggcctacct gttcgaggat
     4441 agcaatagat cacgacgcgg ccagctaaac tcgccaaatc tacgaggtgt ccagatgtag
     4501 accggagcgc aaccgacggc aaacgcatgt tggtgagatg gttgcaagcc ccatcatcaa
     4561 ctggcacagg tacgttatcg ggaatctcgt aaaggttatc gcttctcggc ataagcaggc
     4621 actcagcgct gttgtacagc catactcact tgaggaccgc atggctggcg ctctcgatca
     4681 gcgcgatgat ctcatccggc tggttggcga cgagatggtc tccagcgaat atcactgagg
     4741 cgctacccca gacggcctgg actcccaaga ctctcgcgtt cctcgcgcac gcaacatcca
     4801 cctcggcgtc accgacgtaa gcagcttcct ccgttctcac gcccaacctc cggcaaacta
     4861 tctccagacc atcaggggcg ggcttggagt gagtgacctc gtcaccggcg acaattgccc
     4921 cgaaatgccc atcgaggcct gtcctgggaa gaacaaggtt cacggcgcgc cttgtggcgc
     4981 tcgtgaacag gccgaggcgg tagcccttcc ggtgaagttg cttgagcatg cctgccacac
     5041 gtgggaaagg ctggagcctc gatatcgctg cctcgtaggc gacgtagtaa gcatcgagat
     5101 cctgaggcaa gattggacga ccaagaaaac gttcaaggat gacgggcgtc gggccgatgt
     5161 tgaaattgga gagaatgtcg tacgtcgtga cgtcgcctct gccaagcgag cggatagtct
     5221 ccacgtacgt gttcaggatc aagcctgtag tgttgaccag ggttccgtca aggtcaaaca
     5281 cgactgctct catgtcacct caccagccgg ctctccgaga gccaggtccg gggccaggtc
     5341 tgacaatatg ctcggcggtt
//
LOCUS       DBNHXB010000317         8499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4447247, whole genome shotgun sequence.
ACCESSION   DBNHXB010000317 DBNHXB010000000
VERSION     DBNHXB010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8499
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4447247"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1249)
                     /locus_tag="AC162G_12820"
     CDS             complement(158..1249)
                     /locus_tag="AC162G_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5076733.1"
                     /translation="MFRLPAGTYFQTYQQAHRFWPTRFMTLRMIGLLVVLFVAVPLVA
                     NNYVLSIANRIGYTILAAMGVQLLIGFTGQLTLGHAAFVAVGAYASAIFTLTAVENAD
                     IPFWGFLTQAGLAYPLSVVVACLLSGLWSVLFGLPSARVKGFGIILTTMAAQFITVNF
                     IIAQYVSQIGGRGHSITLPLGTVKIGPWALAGDRDAYFWMLILVVLVTAAMNNWKRTR
                     PGRAWLAIRDNDIAAEVLGVNIVRYKLLAYFAAGILAGLAGSFWITSLFFVSPEHFEW
                     SNSLWWVGVILIGGVGAVDGTIFGAIFLELMLVLVNSFPAANIYFQYFKDIAFGLAII
                     LFLLYEPKGLAYRWWQIKNYFYLWPFSYK"
     gene            complement(1329..2213)
                     /locus_tag="AC162G_12825"
     CDS             complement(1329..2213)
                     /locus_tag="AC162G_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006320798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5076734.1"
                     /translation="MELFVQLMVTGTLVGSIYALVALGWTLIYKSSGVLNLAMGELTL
                     IGAYVCLTFYNWGIPFPLAILGSLIIGGVLGLLVERLVLRPLIGEPILTVIMVTVGMS
                     FLFRGLVGLIWGTDTQVFTPPVLPAEPIRVGPITISPVFLGGFAAALILLVVFVLFFQ
                     RTRWGLAMQATADDQMAALSLGVSAKLVYAIAWAIAFMAAGVGGIFLGIINGLNVSVS
                     FLGLLVLPAVVLGGLNSVPGAIVGGVLIGILQNLAGGYLDQLIPGGIKEVFPLAFMAV
                     VLLFKPYGLWGWQKIERV"
     gene            complement(2253..4211)
                     /locus_tag="AC162G_12830"
     CDS             complement(2253..4211)
                     /locus_tag="AC162G_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5076735.1"
                     /translation="MPPADSYPQGELTITPDLTLPKLMLQTARRLGDRHVALREKEYG
                     IWRPITWREYVEAVREIALGFVALGLDRGDKVALIGDNRPEGLFAEMAALAVGGVAVW
                     LFQDSLIDEVQYIVDHCDAKILVGEGQEEADKGLAIRDRCPKLEKIVWDDPRGMRHYD
                     DPMLIGLPDARRLGRELDEREPDLFKRLVEQGQAEDVCLLFYTSGTTAKPKGALLTHH
                     NMLKMGQNLLRVDAYRQSDDFVSYLPFAWIGEQMMSISCGLQAGFTLNFPEEPETSQD
                     NIREIAPHMLFAPPRIYEQMIRSVQVRYLDASRLKRKAYELGMAIGYRVADAKFARTP
                     LPAAWRLLRLVGEGLLFRSLRDHLGLSRARYTYSGGAALGPDHFRFFHAIGVNLKQIY
                     GQTEIAGISVLHRDGDVKFDTVGKPIPETEVQITLEGEIIARSPSVFQGYYKNPEATA
                     QALRPNPGGSGLWLHSGDTGFIDQDGHLVVFDRSGDVMILSDGSKFSPQYIESRLKFS
                     PYLQDAWVLGDQRPFVTAVICIAYNVVGKWAEERNIAYTSYTELSQKPEIYTLVGQAI
                     REVNRRLPDTARIRRFVNLYKAFDADDDELTRTRKLRRRFLEERYQDIVRALYADQDA
                     VHIDTTITYEDGRVAPIKTDLRVEPVGE"
     gene            complement(4195..5007)
                     /locus_tag="AC162G_12835"
     CDS             complement(4195..5007)
                     /locus_tag="AC162G_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076736.1"
                     /translation="MPDQLSETLLKVEGLSLTFGGVAALIGVGLEVHRGEIFSVIGPN
                     GAGKTSLLNCISGLYHPQRGKIVFRGRDISRFSAHRRAALGLSRTFQKIELFKGMTVL
                     DNIRLGRHLRMKTGLLDGALWIGRASREEVEHRAFIEEAIVDLLEIEHLRDHVVGALP
                     YGLQKRVELARALALEPQLLLLDEPMAGLNLEEVEDMVRFILDINEDPRWNVTCILVE
                     HDMGVVMDISDRVAVLNFGEKIAEGTPADAQATPAVIHAYLGEHEGLYATRR"
     gene            5690..5911
                     /locus_tag="AC162G_12840"
     CDS             5690..5911
                     /locus_tag="AC162G_12840"
                     /inference="COORDINATES: protein motif:HMM:NF024835.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5076737.1"
                     /translation="MLLGRQQAVTGERLTLRRLASLANVPKDLVYRLDAGQARHVDLD
                     ALARLCQALSCEPSDILVWDNDGRDREPV"
     gene            5886..7334
                     /locus_tag="AC162G_12845"
     CDS             5886..7334
                     /locus_tag="AC162G_12845"
                     /inference="COORDINATES: protein motif:HMM:NF039513.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wadjet anti-phage system protein JetA family
                     protein"
                     /protein_id="HIR5076738.1"
                     /translation="MAETANPFNVLPANLFNLFSTQGATTLQRHYMAILLRVYGLAEF
                     SRAGLSREVVIGEIVDYLRSAGAEGEVAAEMAEADEEVRSSITGQLTEHDYAAYLLRR
                     LAETGWVEREQHADYSETIILPDYAFTLLEAFRAIQEQKPREFAGQLYTAHQLISGSR
                     SKDFSPALAVTQAYENVRQVVRGLNELNHNIRRYIERATRGHSPSELLQLQFDDYART
                     LGHAYHALKTSDHVSRYRRDIVARLRSWQRNREWLDRTAEELAAQGRFTPAQAAEEID
                     RYMGFIIHQLEGLDPLLAEIDRRHAQYLRTSLRQIRYHIVSADGSFKDRLASLARHLA
                     ALLNEGETTLPEGVPGLQLHSVRAPDGNSFFTPPRRRPPFVTETIASPVLSLSDLASL
                     RLATLQDVTQALTPDRVNRTVMGFFNGHRALHVSELPASLLDDMHSFTTIIAYSHHPD
                     VEYGLECVPGDPVPIGPYRVAPFQLYKLAADH"
     gene            7375..8028
                     /locus_tag="AC162G_12850"
     CDS             7375..8028
                     /locus_tag="AC162G_12850"
                     /inference="COORDINATES: protein motif:HMM:NF025205.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HIR5076739.1"
                     /translation="MDTDTLLESAGLSDKARRDVPRVINRLLGQTFLHQDVDADKDDY
                     YFVHRYRPVFESLLRLCGFNLLHDDYHRIYQAVSDFSYCRAHFRLDETLMIVVLRKLY
                     EERIEHLSLANDPVVSIGEVREEYRTITGKERDLGIVQYQDLLRRLRAKGLIDTLDGA
                     RGAIDVRNTDARLRLRGSVRMILPVKNADEMEAWLRKYRAMDGETPSQDDIDSDEPF"
     gene            8067..>8499
                     /locus_tag="AC162G_12855"
     CDS             8067..>8499
                     /locus_tag="AC162G_12855"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5076740.1"
                     /translation="MKLLTRIRLINWHLFENATISCQGTTYFIGVNGVGKSTILDAVQ
                     FALVGGQRDVKFNQAALSSGKRTLAGYVRGEIGAEGRDFLRGDATSVVALEFRNPDGA
                     HFVHGAVIDAYEDSRSPDVIYFIVHNAALDYDWFFSAPGQLF"
BASE COUNT         1731 a         2697 c         2537 g         1534 t
ORIGIN      
        1 gctcgtttct gcctgcacaa tctaggcccg gtgggccatg cgttaattga taaccttgaa
       61 aacacgtctg acaaggggta cagatctcca agactttagg ggtacgatat tacaagactc
      121 aacaacgttt tacgcttcac acccggtttc acctccttca cttgtacgaa aacggccata
      181 agtagaagta gttcttgatc tgccaccagc ggtaggccag ccccttaggc tcgtacaaca
      241 ggaacaggat gatggccagg ccgaaggcaa tatccttgaa gtactggaag tagatgttgg
      301 ccgccggaaa cgagttgacc agaacgagca tcagctccag gaagatcgcg ccgaagatcg
      361 tgccgtctac cgcgcccacg ccgccgatga gaatcacgcc cacccaccat aacgagttgc
      421 tccactcgaa atgctccggg ctgacgaaaa acagcgacgt gatccagaac gagccggcca
      481 ggccggcgag gatgccggcg gcaaagtagg ccagcagctt gtagcggacg atgttcacgc
      541 ccagcacctc ggcggcgatg tcgttatcgc ggatggcgag ccaggcccgt cccggccgcg
      601 tccgcttcca gttgttcatc gccgccgtca ccagcacgac caggatcagc atccagaaat
      661 acgcatcccg gtctccggcc agcgcccagg ggccgatctt gaccgtgccc agcggcagcg
      721 taatagagtg tccccggccg ccgatctggc tgacgtactg ggcaatgatg aaattgaccg
      781 tgatgaactg ggcagccatc gtggtcagga tgatgccaaa ccccttgacg cgcgccgagg
      841 gcaggccgaa taatacgctc cacagaccgg agagcaaaca cgctaccacg acactgaggg
      901 gataggccag gccggcctgc gtcaggaagc cccagaatgg aatatcggcg ttttcgacgg
      961 cggtgagtgt gaagatagcg ctggcgtaag cgccgacggc gacgaaagcc gcgtggccca
     1021 gcgtcagttg cccggtgaag ccgatgagca attgcacgcc catggcggcc aggatggtgt
     1081 aaccgatgcg gttggcgatc gacagcacat agttgttcgc gaccaaagga acagccacga
     1141 acaacacgac cagcagaccg atcatccgca aggtcatgaa gcgggtcggc cagaagcggt
     1201 gcgcctgctg gtaagtctga aaatacgtgc cggccggcag tcggaacatg cctccaatct
     1261 ccaatctcta atctctaatc tccaacttcc aacctctaat ctctaactcc caactctaac
     1321 tcccaactct acaccctctc aatcttttgc cacccccaca acccgtaggg cttgaagagc
     1381 agcacgacgg ccatgaaggc caaagggaag acttccttga tgcctccggg aatgagctgg
     1441 tcgaggtagc cgccggccag gttctgcaaa atgccgatga gcacgccgcc tacaatcgcc
     1501 ccgggaaccg agttcaggcc acccagcacg accgccggca gcacgagcag cccgaggaaa
     1561 ctgaccgaga cattcagccc attgatgatg ccgagaaaga tgccgcccac gccggcggcc
     1621 atgaaggcga tcgcccaggc gatggcatac acgagcttgg cgctgacgcc cagcgagagc
     1681 gccgccattt gatcgtcggc ggtggcctgc atcgccaacc cccagcgcgt gcgctggaag
     1741 aacaagacga agacgacgag caggatcagg gctgccgcga agccgcctaa gaagaccggg
     1801 ctgatggtga tcggcccgac gcgtatcggc tctgccggaa ggacgggggg agtgaacacc
     1861 tgtgtatcgg tgccccagat caacccgacc agtccccgga acaggaacga catacctacc
     1921 gtgaccatga tcacggtcag gatcggctcg ccgatcagcg ggcgcagcac caggcgctcg
     1981 accagcagcc ccaacacacc gccgatgatc agggagccga gtatcgccag gggaaaggga
     2041 atcccccaat tgtagaaggt caggcagaca taggccccga tcagggtcag ctcacccatc
     2101 gccaggttga gcacgccgct gcttttgtaa atcagcgtcc aacccagcgc caccagcgcg
     2161 tagatgctgc cgaccagggt tcccgtgacc atgagctgca caaagagttc cataccttca
     2221 aaccccaaac tctaactcta actcccaact cgctactcgc ccaccggctc aacccgcaga
     2281 tcggtcttga tgggtgcgac gcgcccgtct tcgtaagtga tcgtcgtgtc gatgtgaacg
     2341 gcgtcttgat ccgcgtacag cgcccgaacg atgtcttgat agcgttcctc caggaagcgc
     2401 cgccgcagct tgcgcgtgcg cgtcaactcg tcgtcgtcgg cgtcgaatgc cttatacagg
     2461 ttgacgaagc gccggatgcg cgcagtgtcg ggcaggcgtc ggttcacctc ccggatggcc
     2521 tgcccgacca gtgtatagat ttccggcttc tgtgaaagct cggtgtagga ggtataggcg
     2581 atgttccgct cctccgccca tttgccgacg acgttgtagg cgatgcagat gacggcggtg
     2641 acgaagggtc gctgatcgcc caacacccag gcgtcttgca ggtaggggct gaacttcaac
     2701 cggctctcga tgtactgagg cgagaacttg ctcccgtccg acaggatcat gacgtcgccc
     2761 gatcggtcga acacgaccag gtgaccatcc tgatcgatga agccggtgtc gccggagtgc
     2821 agccaaagtc cgctcccgcc cggattgggg cgcagcgcct gggccgtcgc ctccgggttc
     2881 ttgtaatagc cctggaagac cgacgggctg cgggcgatga tttcgccttc cagcgtgatc
     2941 tgcacttcgg tttcggggat cggtttgccg accgtgtcga acttgacgtc gccgtcgcgg
     3001 tgtagaaccg agatgccggc gatctcggtc tggccgtaga tttgcttgag gttgacgccg
     3061 atggcgtgga agaagcggaa gtgatccggg ccgagcgccg cgcctcccga atacgtgtaa
     3121 cgcgcgcgag ataggccgag gtggtcgcgc agcgatcgaa acaggagccc ttcgccgacc
     3181 agacggagca gacgccaggc ggccgggagc ggcgtgcggg cgaacttggc gtcggccacc
     3241 cggtagccga tggccatgcc cagctcgtac gccttgcgct tcaaccgcga cgcgtccagg
     3301 taacgcactt gcacgctgcg gatcatctgc tcgtagatgc gaggcggggc gaacagcatg
     3361 tgcggcgcga tctcgcggat gttgtcctgg ctcgtctccg gctcttcggg aaaattgagc
     3421 gtgaagccgg cttgcaggcc gcaagagatc gacatcattt gctcgccgat ccaggcgaac
     3481 gggaggtagg agacgaaatc gtcgctctgg cggtacgcat ccacccgcaa gagattctgg
     3541 cccatcttca gcatgttgtg gtgagtcagc agcgcccctt tgggcttcgc cgtggtgccc
     3601 gaggtgtaaa acagcaggca cacatcctcg gcctggcctt gctcgaccag gcgcttgaac
     3661 aggtctggct cgcgctcatc cagctcccgc cccagccttc gggcatcggg caaaccgatc
     3721 agcatcgggt cgtcgtagtg acgcatgccc ctggggtcgt cccagacgat cttttcgagc
     3781 ttggggcacc ggtcgcggat ggctagaccc ttgtccgcct cctcttgtcc ctcgccgacc
     3841 aggattttgg cgtcgcagtg atcgacaatg tactgcactt cgtcgataag cgagtcctgg
     3901 aagagccaga cggcgacccc gccgacggcc agtgccgcca tttcggcaaa gaggccctcg
     3961 ggccggttgt cgccaatgag ggcaaccttg tccccccggt cgaggccgag ggcgacgaag
     4021 cctaatgcga tttctctcac ggcttcgacg tactcgcgcc acgtgatcgg gcgccagatg
     4081 ccgtattcct tctcacgcag ggcaacgtgc ctatcgccca gccgccgcgc cgtctgcagc
     4141 atcaacttgg gcagcgtgag gtctggggtg atcgtcaatt cgccctgcgg atagctatcg
     4201 gcgggtggca tacaagccct cgtgttctcc aagataagcg tgaatgacgg ccggggtggc
     4261 ctgggcatcg gccggagttc cctcggcgat tttctcgccg aagttgagca cggcgacccg
     4321 gtccgaaata tccatcacga cgcccatatc gtgctcgacg agaatgcacg tcacgttcca
     4381 gcgtgggtct tcgttgatgt ccaggatgaa gcgcaccata tcttcgactt cttccaggtt
     4441 tagtccggcc atgggttcgt cgagaagcag caactggggt tcgagcgcca gagcacgggc
     4501 gagctccacg cgcttctgca atccgtaagg cagcgcgccg acgacgtggt cacgcaggtg
     4561 ctcgatctcc aggaggtcga cgatcgcctc ttcgatgaag gcccggtgct cgacttcctc
     4621 gcgggaggcg cgcccgatcc atagcgcgcc gtcgagcagg ccggttttca ttcgcaggtg
     4681 gcgtccgaga cgaatgttgt cgaggacggt catgcctttg aacagctcta ttttttggaa
     4741 ggtgcgcgag aggcccagcg cggcccgccg gtgtgcggag aagcgcgaaa tgtcacgccc
     4801 tcgaaagacg atcttcccgc gctgagggtg atacaggccg ctgatgcaat tcagcaacga
     4861 agtcttgccc gcgccgttcg gcccgatgac ggagaaaatc tcgccccgat gcacttccag
     4921 gccgactccg atgagggccg ccacaccgcc gaaagtcagg gagagacctt caactttcaa
     4981 cagtgtttca gatagctgat ccggcacgtc tcctccagga aatcttcctg ccgggtgcgc
     5041 gtacttcgac agtcacattt aggctcaagc cgccgtcccc gagtgcggcg cggcgagccg
     5101 aatctgacat tgtcaaggca ctatgccagc gattctgtta gcacaagcgg gtttggagac
     5161 ggattgaccg tcgctcgcca cgaagcatct cgcccggcac gatggcctgc gtgccctgga
     5221 gggtgaagga tgcgaagcga gacgacaata ttagagatag tataggaatt tttatgcgga
     5281 tgtcaatgtg tttaagcact aaaacattgt gactactatc ctgtgcactg gagtttggcg
     5341 acagccatat ctggcgatcg gagtgcaggc ttcagcctgc gaagcgccca gatatggcag
     5401 cctgacccca ctccgctttc tttcggctgc gctcagggca aggctcagga caggcgctgc
     5461 ggggcaagtg aggctgcgct ccgggaattc gctagactcc aactgtgtaa acagtcgctt
     5521 cgggcgaagt ggcatctcgg cgctgagtca acaagcggat gccaattgcg ccatttgtct
     5581 taaaaaatcg cacaagcatc ttgacaaacc ggtcgatctg tcgtatacta cgacagtctt
     5641 agaacgcacg ttctatatgc ccaggctcac tacttcaggt cgaatcacca tgctccttgg
     5701 caggcagcag gccgttaccg gcgagcgact gacgctgcgc cgtctggcct cacttgccaa
     5761 cgtgcccaag gacctggtct atcgcctcga cgccggccag gcgcgccacg tcgatctcga
     5821 cgccctcgca cgcctgtgcc aggcgttgag ctgcgagccg tcagacattt tggtgtggga
     5881 caacgatggc cgagaccgcg aacccgttta acgtcctgcc ggccaacctg ttcaacctgt
     5941 tcagcacgca gggcgccacg accttgcagc gccattacat ggcgatcctg ctgcgcgtct
     6001 acgggctggc cgagttcagc cgcgccggcc tatcgcgcga ggtcgtgatt ggcgagattg
     6061 tggattacct gcgcagcgcc ggcgcggagg gcgaggttgc cgcagaaatg gcagaggccg
     6121 acgaggaggt gcgcagctcg atcacgggcc agttgaccga gcacgactac gccgcttatc
     6181 tgctgcgccg tctggccgag acgggctggg tcgagcgcga gcagcacgcc gactactccg
     6241 agacgatcat cctacccgat tacgccttca cactgctcga agcgttccgc gccatccagg
     6301 agcagaagcc gcgcgagttc gcgggccagc tctacactgc gcatcaactg atcagcggct
     6361 cgcggagcaa ggatttctcc ccggcgctcg ccgtcacgca ggcttacgaa aacgtgcggc
     6421 aggtcgtgcg cgggctgaac gagctgaacc acaacattcg acgctacatc gagcgcgcga
     6481 cgcgcgggca cagcccgtcg gagctgctgc aactacagtt cgacgactac gccaggacgc
     6541 tcggccacgc ctatcatgca ttgaagacat cggatcacgt atcgcgttat cggcgcgaca
     6601 tcgtcgccag gctgcgctcg tggcagcgca atcgcgaatg gctggatcgg acggcggaag
     6661 agcttgccgc gcaggggcgc ttcacgccgg cacaggccgc cgaagagatc gaccgctaca
     6721 tggggttcat catccatcaa ctcgaagggc ttgaccccct gctcgccgag atcgatcgcc
     6781 ggcacgcgca gtatctccgt acatcgctgc gccagattcg ctaccacatc gtgagcgccg
     6841 acggcagttt caaggaccgc ctggcgtcgc tggcgcggca tctggccgcc ttgctgaacg
     6901 aaggcgaaac gactctgccc gaaggcgtcc ccggcttgca gctccattcc gtgcgcgcgc
     6961 ccgacgggaa cagcttcttc acgccgccca ggcgacgccc cccgttcgtc acggagacga
     7021 tcgcttcccc ggtcttgtcg ctgagcgatc tggcctcgct gcggctcgcc acgctgcagg
     7081 atgtgacgca agctctcacg ccggacagag tcaaccgaac ggtgatggga ttcttcaacg
     7141 gccaccgcgc gctccacgtc agcgagctgc cggcgtccct cctcgacgac atgcactcgt
     7201 tcacgaccat cattgcctat agccaccatc ctgacgtcga atacggtctc gaatgtgtgc
     7261 ccggcgatcc cgtcccgatt ggcccgtacc gcgtcgctcc cttccagctt tacaaactgg
     7321 ccgccgacca ctgatcaccg atcactgatc actgattact gactactgaa caccatggat
     7381 accgacaccc tcctcgaatc cgccggcttg agcgacaaag cgcgccgcga tgttccgcgt
     7441 gtgatcaatc gtctgttggg gcagacattc ctgcatcaag acgtagacgc ggacaaggac
     7501 gactactact tcgtccaccg ctaccggccg gtattcgaga gtctgcttcg actgtgcggg
     7561 ttcaacctgc tgcacgacga ctaccatcgc atctatcagg ccgtctcgga cttcagctac
     7621 tgccgcgcac atttcaggct ggacgagacg ctcatgatcg tcgtcctgcg caagctgtac
     7681 gaagagcgca tcgagcacct cagcctggcc aacgatcccg tagtgtccat cggcgaagtg
     7741 cgcgaggagt accgcacgat cacgggcaag gagcgcgacc tgggcatcgt acagtaccag
     7801 gacctgctgc gccgcctgcg ggcgaagggg ctgatcgaca cgctggacgg cgcacgcggc
     7861 gcgatcgacg tgcgcaacac ggacgcacgg ctgcggctgc gtgggtcggt gcgcatgatc
     7921 ttgccggtca agaacgccga cgaaatggaa gcctggctgc gcaagtaccg cgccatggac
     7981 ggagagacac cgtcacaaga cgacatcgac tcagatgaac ccttctgatc accgatcact
     8041 gatcactgat tactgactac tgaccaatga aactcctcac ccgcatccgc ctgatcaact
     8101 ggcatttatt cgagaacgcc accatctcgt gccagggcac gacctatttc atcggcgtca
     8161 acggtgtcgg caaatcgacc atcctcgacg ccgtgcagtt cgcgctcgtc ggcgggcagc
     8221 gcgacgtgaa attcaaccag gcggcgctga gcagcggcaa gcgcacgctc gccggttacg
     8281 tgcgcggcga gatcggcgcc gaaggccggg acttcctgcg cggcgacgcg accagcgtcg
     8341 tcgcgctcga gttccgaaac cccgacggtg cacacttcgt ccacggcgcg gtaatcgatg
     8401 cgtacgagga tagtcgcagt cccgacgtga tctacttcat cgttcataac gccgcgctcg
     8461 actacgactg gttcttcagc gcgcccggcc agttgtttg
//
LOCUS       DBNHXB010000318         9091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4112055, whole genome shotgun sequence.
ACCESSION   DBNHXB010000318 DBNHXB010000000
VERSION     DBNHXB010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9091
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4112055"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="AC162G_12860"
     CDS             <1..749
                     /locus_tag="AC162G_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR5076741.1"
                     /translation="GFLLGDATKAAAEANPDQKFQILDSAYDPVIPNVWGQVYATDQG
                     AFLAGYVAAAVTKTGKVGTFGGINIPPVTDFMDGFALGVAYYNQENDTNVEVLGWDVE
                     AHDGLFTGNFESTDDGRRMGETLMDEGADIIMPVAGPVGLGTAAAVQERGGAYIVGVD
                     TDWTVSAPEYADIVLTSVEKRLDLSVLSAAKAVAAGIFAGGTQLATLANGQIGISPFH
                     SLDSMVPDEVKADLEQITADIIAGTIQTKP"
     gene            895..2460
                     /locus_tag="AC162G_12865"
     CDS             895..2460
                     /locus_tag="AC162G_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076742.1"
                     /translation="MPPVLELRNITKRFPGVLANDHISLTLEPGEIHALLGENGAGKT
                     TLMNVLYGLYHPDEGEIIVRGKPIKVASPTDAIRVGIGMVHQHFMLVPVLSVTENVML
                     GDEMLTGGVFLDLRRTAERIRSISKQYGLEVDPDALVKDLPVGVQQRVEIIKLLFRQA
                     DILILDEPTAVLTPQEVTELFKVIRSLVDQGKSIIFITHKLKEVLALADRITVLRVGQ
                     VVGTTTPAEASEARLAAMMVGREVMLEVQKEPAQPGEVVLQVEDLEVLDDRLNVAVDG
                     VSFEVRTGEVLGVAGVQGNGQTELVEALTGLRAPLEGTVRILGHDTTHATPRRITELG
                     VAHVPEDRQRDGLVLTYPVSANMVLSTYYLPPFARGMVLQDSAIDAAAMERIKQFDVR
                     APGIDTSAGSLSGGNQQKVIVAREFSRPIKLLIASHPTRGLDVGSIEYIHSRIIQKRD
                     DSVGVLLVSPELDEIIALADRIAVMYRGRIVAIVDADAATKEYLGLLMAGVDPATLSP
                     PQKRETRKVEVVF"
     gene            2460..3920
                     /locus_tag="AC162G_12870"
     CDS             2460..3920
                     /locus_tag="AC162G_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076743.1"
                     /translation="MAQTDTTVSTPKAASPMPKTSRWRQFWGIAQLPVLAFVMAILVG
                     GVIIALSDFTVLEELTKAVNSLNFVGLALTIAAIAALAGGVYVSRNTEYAAKRLAIRT
                     LTPWQGRVLSLATLVGGVVLPILFLRAAGFGPVLDAAWVAVITAYGALFEGSIGNPAQ
                     IWSALQSGDATAISSAFYPISESLVATTPYIFAGLAVAIGFRAGLFNIGAEGQLFMGA
                     IFAVFVGYSLTGLPWIIHLPLTLAAGALGGAAWGFIPGILKAKVGAHEVINTIMMNYI
                     AFRLSDWLLTGPMQRPGSVPVSPMIEPSAYLPRFFQDPLRFHGGFFLALAVAAFVYWL
                     LFRTTRGFELRTVGQNPSAARYAGMSITRNIVLTMCLSGALAGLAGANEVLGVNHNLA
                     QAFSSGYGFDSIALALLGKSHPLGVVLAALLFGTLRSGGTRMQNVAQIPIDIIAVVQA
                     LVIVFIAAPAIIRAIYRVREERAIGEVFTRGWGAKT"
     gene            3917..5245
                     /locus_tag="AC162G_12875"
     CDS             3917..5245
                     /locus_tag="AC162G_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008335183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076744.1"
                     /translation="MTANALAKTTRRLTPAQAAELAQRTRYRVIGALLVLLGVAGYVL
                     FANRVDPSAVSTFGMNTGSAGAVRLDDWVVPTYSLITLLSGVSLFVGAFQFARGFARW
                     TNAVFGLVILMFLFSFLTWATADKSINLAALIKNSLLRSVPLTLGALSGVLSERAGVV
                     NIAIEGMMLSGALAGTIVGSLAAQWFPPEQYGALSLWIGTLAAVLNGLLLSLVHAVLS
                     IRYKVDQIISGVVINIFSTGITSFISAKFLSEFAQLNNPGIFPPTPVPALSQIPFLGG
                     ILFDSNMFTYAMYGLLIVVHVALFYTRWGLRTRSVGEHPKAADTLGINVFRTRYVAVM
                     LSGMMAGFAGAYFTLGSVGRFDELMTAGRGFIALAAMIFGKWTPFGAFGAALVFGFAD
                     SLQSRLAILRVPIPSEFLLMAPYVATMIALAGLIGRATPPAADGLPYEKE"
     gene            5309..5650
                     /locus_tag="AC162G_12880"
     CDS             5309..5650
                     /locus_tag="AC162G_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006519075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5674 family protein"
                     /protein_id="HIR5076745.1"
                     /translation="MASVVILHEPPSPEQLNELLAVFETFIKLAVDVRREVVAAGGEM
                     HADCEEALLEDGSRQEDVWGADWIPADRTVRFEALINIRPRQSNLQMEIQDPQLRAQV
                     ERVVRRVFEGV"
     gene            5654..6013
                     /locus_tag="AC162G_12885"
     CDS             5654..6013
                     /locus_tag="AC162G_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076746.1"
                     /translation="MDWRKLRERYVRDNWPRRLGNLASTLGRVASATNNPNTLTTVPP
                     SLRESMLMIEWNLQRTPSEILIELAAMQAELHLWWRGWEAVAPSPALRDLLSRRARQM
                     SDRVLELSGLLTSNSPL"
     gene            6030..6755
                     /locus_tag="AC162G_12890"
     CDS             6030..6755
                     /locus_tag="AC162G_12890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5076747.1"
                     /translation="MNSTRLYTDLASWWPLLSSPADYAEEAAFCRQLLMDACNRPPRT
                     VLELGSGGGNNASHLKAHFQLTLVDTSPGMLAVSRALNPECEHIQGDMRSVRLDRLFD
                     AVFVHDAIEYMTTESDLRAAMETAFTHCRPGGAALFVPDHVRETFQPETKHGGHDGDG
                     RSMRYLEWTTDPDPSDTTYLTDFAYLLREADGSVRVEHDRHVCGLFGREDWLRLLHEV
                     GFQPGAVVDNYSREEFIAVKPGA"
     gene            6775..7362
                     /locus_tag="AC162G_12895"
     CDS             6775..7362
                     /locus_tag="AC162G_12895"
                     /inference="COORDINATES: protein motif:HMM:NF016001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR5076748.1"
                     /translation="MTILDPRVEESLRRGFKKVLNPFMLTIWRLGLGPWFNVWPDVSG
                     RVVVITHIGRKSGAARRTPVNYAIVGGDIYCVAAFGAISDWYRNIRANPNVEVWLPDG
                     WWAGTAEDVSDDPRRLLLMRQVMIGSGFAARVAGINPYAIGDAELDATTAKYGLIRIR
                     RSEARTGPGGPGDLAWVWPVATIILLSLALFRRKR"
     gene            7912..>9091
                     /locus_tag="AC162G_12900"
     CDS             7912..>9091
                     /locus_tag="AC162G_12900"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF002436.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR5076749.1"
                     /translation="MKANELRERYQQFFEARGHRRIRSAPLLPENDPTVLFTTAGMHP
                     LVPFLLGEPHPLGRRLVNVQKCIRTNDIDEVGDVSHLTFFEMLGNWSLGDYFKPESLA
                     WSYEFVTQELGIDPARLAVTVFEGDDDAPRDDVSAGIWRGLGIPGERIFFLPKADNWW
                     GPAGATGPCGPDSEIFVDTGRPDHPGCRPGCSCGKWFEIWNNVFMEYNKTAGGRYVKL
                     QQQNVDTGMGVERTLTVLNGYDDTFKIETIWPLVERIEQLSGRRYAGNPVPFRVIADH
                     LRASTFAIADGATPSNVEAGYVVRRLIRRMVRYGRDLGIQRNFCAELSSVVVALFAGV
                     YPETEQNHARIQDELDREETKFKRTLERGLREYHKVAGRTAGAGSRAISAADAFNLFE
                     T"
BASE COUNT         1597 a         2868 c         2943 g         1683 t
ORIGIN      
        1 tcggcttcct gctgggcgac gcgacgaagg cggcggcgga agcgaacccg gatcagaagt
       61 tccagatcct ggactccgcg tacgatccgg tgattccgaa cgtgtggggt caggtgtacg
      121 cgacggacca gggcgcattc ctggccgggt acgtggccgc ggcggtgacg aagaccggca
      181 aggtcggcac cttcggcggc atcaacatcc cacccgtcac cgacttcatg gacggcttcg
      241 cgctcggtgt tgcctattat aaccaggaga acgacacgaa cgtcgaagtc ctcggctggg
      301 atgtggaagc ccacgacggt ctgttcacgg gcaactttga gagcacggac gacgggcgcc
      361 ggatgggtga gacgctgatg gacgagggcg cggacatcat catgccggtg gccgggccgg
      421 tcggcctggg cacggcggca gccgtgcagg agcgcggcgg cgcctacatc gtcggcgtgg
      481 acaccgactg gacggtgagc gcgccggagt acgccgacat cgtcctcacc agcgtcgaga
      541 agcgcctcga cctgagcgtc ctctccgcgg ccaaggccgt cgcggccggc atcttcgcgg
      601 gcggcacaca gctcgccacg ctggcgaacg gacagatcgg catctcgccg ttccacagcc
      661 tggacagcat ggtgcccgac gaggtcaagg ccgacctgga gcagatcacg gcggacatca
      721 tcgcgggcac gatccagacc aagccgtgac cccaaagacc tgtgaggtct tacagacctc
      781 acaggtctgt tatggaatcg tcggcggcga ggcgagactg actctccgcc gattttatct
      841 acctggaaag actcgacggg tctcggagac ccgtcgggtc tgggtgaggt tcttatgcct
      901 cccgttctcg aattacgcaa catcacgaaa cgcttccccg gcgtgctggc caacgatcac
      961 atctccctga cgctggagcc gggcgagatt cacgcgctgc tcggcgagaa cggcgccggc
     1021 aagacgacgc tgatgaacgt cctctacggg ctgtatcacc ccgacgaagg cgagatcatc
     1081 gttcgcggga agccgatcaa ggtcgccagc ccaacggacg cgattcgtgt tggcatcggc
     1141 atggtgcacc agcatttcat gctcgtgccc gtgctcagcg tcaccgagaa cgtgatgctc
     1201 ggcgacgaga tgctcaccgg cggtgtgttt ctcgatctca ggcggaccgc cgagcgcatt
     1261 cgaagtatct caaagcagta tggcctggaa gtcgatcccg atgcgttggt caaggacctg
     1321 ccggtcggcg tgcagcagcg cgtggagatc atcaagctgc tcttccgcca ggccgacata
     1381 ctgatcctgg acgagccgac ggcggtgctc acgccgcagg aagtcacgga gctattcaaa
     1441 gtcattcgat cgctcgtcga tcagggcaaa tcgatcatct tcatcaccca caagttaaag
     1501 gaagtgttgg cgctggccga tcgcattacc gtgttgcgag tgggacaggt cgtcggcacg
     1561 acgacgccgg ccgaggcgag cgaggcccgg ctggcggcga tgatggttgg gcgcgaggtc
     1621 atgctcgaag tccagaagga gccggcccag ccgggcgaag tggtgctgca ggtcgaggac
     1681 ctcgaagtac tcgacgatcg gctcaacgtt gccgtggacg gcgtgtcctt cgaggtgcgc
     1741 accggagaag tgctgggcgt cgcgggtgtg cagggtaacg ggcaaaccga gctggtcgag
     1801 gcgctcaccg ggctgcgcgc gcccctcgag ggcactgttc gcatcctggg gcacgacacg
     1861 acgcacgcga cgccgcgccg catcaccgag ctgggtgtgg cgcacgtgcc ggaggatcgc
     1921 cagcgcgacg ggctggtgtt gacttatccc gtctcggcaa acatggtgct gtcgacgtat
     1981 tatcttccac ccttcgcgag ggggatggtg ctgcaggaca gcgcgatcga cgcggcggcc
     2041 atggagcgca tcaagcagtt cgacgtgcgc gcgccgggca ttgatacatc cgccggctcg
     2101 ctgtcgggcg gcaaccagca gaaagtgatc gtcgcacgcg agttctcgcg gccgatcaag
     2161 ctgctgattg cttcacaccc gacgcgcggc ctggacgtcg gctcgatcga gtacattcac
     2221 agccgcatca tccagaagcg cgatgacagc gtgggagtgc tgctcgtctc gcccgagctg
     2281 gacgagatca ttgccctggc ggatcggatt gccgtgatgt atcgcggccg catcgtcgcc
     2341 atcgtcgacg cggatgccgc cacgaaggag tatctcggcc tgctgatggc cggcgtcgat
     2401 ccggcgacgc tctcgccgcc gcagaagcgc gagacacgaa aagtggaagt ggtgttctga
     2461 tggcacaaac agacacgacc gtatcgaccc ccaaggcggc cagcccgatg cccaagacct
     2521 ctcgctggcg gcagttttgg ggaatcgcgc agttgcccgt gctggccttc gtgatggcta
     2581 ttctcgtcgg cggcgtgatt atcgcactca gcgatttcac ggtgctcgag gaactgacga
     2641 aggccgtcaa cagcctgaac tttgttggcc tcgcgctcac gatcgcggcc atcgccgcgc
     2701 tcgctggcgg cgtctatgtc agccggaaca ccgaatacgc ggcgaagcgc ctggccattc
     2761 ggacgctcac gccctggcag gggcgagtgt tgagcctggc gaccctcgtc ggcggcgtcg
     2821 tattgccgat cctcttcctg cgcgcggccg ggttcggccc cgtgctggat gccgcctggg
     2881 tggcggtcat caccgcttac ggcgccttgt tcgaaggttc aatcggcaat cccgctcaga
     2941 tatggtcggc cctgcagagc ggcgatgcga ccgcgatttc gagtgcgttt tacccgattt
     3001 ccgaaagcct ggtcgcgacc acgccatata tcttcgccgg tctggcggtg gcgatcggct
     3061 tccgcgccgg gttgttcaac atcggggccg aagggcaatt gttcatgggc gcgatcttcg
     3121 cggtgtttgt cggctactcg ttgacgggtc tgccgtggat catccatttg ccgctgacgc
     3181 tggccgccgg cgcgttgggc ggcgcggcat gggggttcat cccgggcatc ctcaaagcga
     3241 aggtcggcgc gcacgaagtc atcaacacga tcatgatgaa ctacatcgcc ttccgcctga
     3301 gcgactggct gctcaccggg ccgatgcagc gaccgggctc ggtgccggtc agcccgatga
     3361 tcgagccgag cgcctacctg ccgcgcttct tccaagaccc gctgcgcttt catggagggt
     3421 tcttcctggc gctggcggtg gccgcattcg tgtactggct cttattccgc acgacgcgcg
     3481 gcttcgagct gcgcacggtc ggccagaatc ccagcgcggc gcggtacgcg ggcatgagca
     3541 ttacgcgcaa tatcgtgctg acgatgtgcc tgtccggcgc actggccggg ctggccgggg
     3601 cgaacgaagt gctcggcgtc aatcacaacc tggcgcaggc tttctcttcg ggctacggtt
     3661 tcgacagcat cgcgctcgcg ctgctgggca agagccatcc gctcggcgtg gtgctggccg
     3721 cgctgctatt cggcacgctg cgcagcggcg ggacgcgcat gcagaacgtg gctcagattc
     3781 cgatcgacat catcgccgtg gtgcaggcgc tggtgatcgt gttcatcgcc gcgccggcga
     3841 tcattcgcgc catctaccgt gtgcgcgagg agcgcgcgat aggcgaagtg ttcacgcgtg
     3901 gctggggtgc taaaacatga cagcgaatgc actcgcgaaa acgacgcgtc gactcacacc
     3961 cgcccaggcg gccgaactgg cgcagcgcac gcgttaccgc gtgatcggcg ccctcctcgt
     4021 gctgctgggc gtggccggtt atgtgctgtt cgccaacaga gtcgatccgt ccgccgtcag
     4081 cacattcggc atgaataccg gcagcgcggg cgcagtcagg ctggatgact gggtcgtgcc
     4141 gacttactcg ttgattacgc tgctgtcggg ggtttcgctc tttgtgggcg ccttccagtt
     4201 cgcccgtggc tttgccaggt ggacgaatgc cgtcttcggc ctggtcatcc tgatgttcct
     4261 gttctccttc ctgacgtggg cgacggcaga caagtcgatc aacctggcgg cgctgatcaa
     4321 gaattcgctg ctgcgatcgg tgccactcac actgggcgcg ttatcgggcg tgctgtctga
     4381 acgggccggc gtggtgaaca tcgccatcga gggcatgatg ctctcgggcg cgctggccgg
     4441 cacgatcgtc ggcagccttg ccgcgcagtg gtttcctccc gagcagtacg gcgcattgag
     4501 cttgtggatc gggacgctgg cagcggtgct caatgggctg ctgctctcgc tggtgcacgc
     4561 cgtcctgtcg atccgttaca aggtggacca gatcatcagc ggcgtcgtga tcaacatctt
     4621 ttccaccggc atcaccagct tcatatcggc caagttcctg agcgagttcg cgcaactgaa
     4681 taaccctggc attttccccc cgacgcccgt gccggccctc tcgcaaatcc cgttcctggg
     4741 cggcattctg ttcgacagca acatgttcac ttacgccatg tacgggctgc tgatcgtcgt
     4801 gcacgttgca ctgttctaca cgcgctgggg cctgcgcacg cgatccgtcg gcgaacatcc
     4861 caaggccgcc gacacgctgg gcatcaacgt tttccgcacg cgctacgtcg ccgtcatgct
     4921 cagcggcatg atggccgggt tcgccggcgc gtacttcacg ctcggctcgg tcggtcgctt
     4981 cgacgagttg atgaccgccg ggcgcggctt catcgcgctg gcggcgatga tcttcggcaa
     5041 gtggacgccc ttcggcgcct tcggcgcggc gctcgtcttc ggtttcgccg actcgctgca
     5101 gagccgattg gcgatcctca gggtgccgat cccgtccgag ttcctgctga tggcccccta
     5161 cgtcgccacg atgatcgcgc tggccggcct gatcggccgc gctacgccgc ccgccgcgga
     5221 cgggctgccg tatgagaagg agtaacggcg ggcagccagg catcaacgaa tcgggcaacg
     5281 aatcaacgaa tcagcgaatc ccctgaccat ggcttccgtc gtgattctgc acgaaccgcc
     5341 cagccctgag cagttgaacg aactcctggc cgtcttcgag acgttcatca agttggccgt
     5401 cgacgtccgg cgtgaagtgg tcgctgcggg cggcgaaatg cacgcagact gcgaggaggc
     5461 tttgttggag gacggcagtc gtcaggagga tgtctggggg gcggactgga tccccgctga
     5521 tcgcacggtg cgcttcgagg cgctgatcaa catccgacct cgacaatcga acctccagat
     5581 ggagattcaa gacccgcaac tgcgtgccca ggtggagcgc gtggtacggc gagtgtttga
     5641 aggagtgtaa gaagtggact ggcgcaagct gcgggaacgc tatgtgcgcg acaattggcc
     5701 gaggcgattg ggcaacctgg cttccacgct gggccgggtg gcgagcgcga caaacaatcc
     5761 caacaccctt acgaccgtgc cgccgtctct gcgtgaaagc atgcttatga tcgagtggaa
     5821 cctgcagaga actccctcgg agatcctcat cgagctggct gcgatgcagg cggaactcca
     5881 tctgtggtgg cgaggttggg aagcggtagc accgtcgcct gccttacgag acctgttgtc
     5941 ccgccgcgcg cgccagatgt ccgatcgcgt cctcgaattg tccggcctgc tgacctctaa
     6001 ctccccactc taactcccca ctctcactca tgaactctac ccggctgtac accgacctcg
     6061 cctcgtggtg gccgctcctc tcgtcacccg ccgactacgc cgaagaggcg gcattctgcc
     6121 ggcagcttct catggatgcc tgcaaccgtc cgcctcgcac cgtgctcgag ctgggcagcg
     6181 gcggcggcaa taacgcctcg cacctcaaag cccactttca acttacgctc gtcgatacat
     6241 cgccagggat gctcgccgtg agccgcgcgc tcaacccgga gtgcgagcac atccaaggcg
     6301 atatgcgttc tgtccggctc gaccgcctgt ttgatgcggt gttcgtccac gacgcgatcg
     6361 agtatatgac gaccgagagc gacctgcgcg ccgcgatgga gaccgccttc acccactgcc
     6421 ggccgggcgg cgctgcgctg ttcgtacctg accacgttcg cgagacgttt caacccgaga
     6481 cgaagcatgg cggccacgac ggcgacggac gaagcatgcg atacctggag tggaccaccg
     6541 atcctgatcc ttcggataca acctacctga ccgactttgc ctatcttctg cgcgaggcgg
     6601 atggatcggt gcgcgtcgag cacgaccggc atgtgtgcgg cttgttcggg cgcgaggact
     6661 ggctgcggct gctgcacgag gtcggatttc agccaggcgc tgtggtcgac aattacagcc
     6721 gtgaggagtt tatcgccgta aagccaggcg cctgaggagt ggcaggagga aagcatgacg
     6781 atccttgatc ctcgcgtcga agagagcttg cgacggggct tcaagaaggt tctcaacccc
     6841 ttcatgctga cgatatggcg gcttggtctg ggcccgtggt tcaacgtgtg gcccgacgtg
     6901 agcgggcgcg tcgtggtcat cacccacatc gggcgcaagt ccggcgcggc ccggcgcacg
     6961 cccgtcaact acgccatcgt cggcggcgac atatattgtg tcgccgcctt cggagcgatc
     7021 tccgactggt atcgcaatat ccgcgccaac ccgaatgtgg aagtgtggct gccggatggc
     7081 tggtgggcgg gaacggccga ggacgtgtcc gacgatccgc gccgcctgct cctgatgcga
     7141 caagtgatga tcggcagcgg cttcgccgcg cgcgtggcag gcatcaatcc ctacgccatc
     7201 ggtgatgcgg agctcgatgc gacgactgcc aagtatggtc tgatccgcat ccggcgcagc
     7261 gaagcgcgca ccggccctgg cggtccaggc gacctggcct gggtgtggcc ggtggcgacg
     7321 atcatcctgc tgtccctggc gctctttcgg cggaagcggt gatccctttt tgtgtgcaat
     7381 caccagacag gcacaccttg tcgaaacgct gcgatgtggg tacaatataa tctaagtggc
     7441 gactgtgaaa gtctttgaca gggacccagc gccgaaaggc ggcgcagaga gccgggcgcg
     7501 ttcctcatcg aacgcatgtg tgagcctggc gccatcacct tcgacgtacg ccaccctcga
     7561 gacgcgagaa gaaagtccgc aggacgagta gacctcgccg ggcgctcccg ttaccgagcc
     7621 gcgctggtca caagggcata acccttgcga aggttcagaa ccttcgcaag gttttatagc
     7681 gaaccgcccg cgcttgagat tcgatccgcg agggtcggat gaattgaggt ggtatcacgg
     7741 gttacggctc gtcctcagca ttgcgctgag gacgagtttt tatttagagc gatttcctaa
     7801 tcgctttacg gatcggagtc gaggctttag ccggaaaccg cctaaaggct cgactcctgg
     7861 gctgtaaatc aaactgaaat ctactctagg tcgatgtgaa ggaagagagc gatgaaggcg
     7921 aacgaactca gggaacgcta ccagcaattc ttcgaagcgc gcggccaccg gcgcatccgc
     7981 tccgcgccgc tattgcccga aaatgatccc accgtcctgt ttacgacggc cggcatgcat
     8041 ccgctcgtcc cgttcctgct cggcgagccg cacccgctgg gcaggcggct ggtcaacgtg
     8101 cagaagtgca tccgcaccaa cgacatcgat gaagtcggcg acgtctctca cctgaccttc
     8161 ttcgagatgc tcggcaactg gtcgctgggc gactatttca agccggagtc gctggcatgg
     8221 agctacgagt tcgtcacgca ggaactcggc atcgacccgg ctcgcctggc cgtgacggtg
     8281 ttcgagggcg acgacgatgc gccgcgcgac gacgtctcgg ccggcatctg gcggggactg
     8341 ggtatccccg gcgagcgcat cttcttcttg cccaaagccg acaactggtg ggggccggca
     8401 ggcgcaaccg ggccgtgcgg gcctgactcg gagatattcg tcgatacagg ccggcccgat
     8461 catcccggtt gccggccggg ctgctcgtgc ggcaagtggt tcgagatctg gaacaacgtg
     8521 ttcatggagt acaacaagac cgccggcggg cgctacgtga agctgcagca gcaaaacgtc
     8581 gacaccggca tgggcgtcga gcgcacgctc accgtgctga acgggtacga cgacaccttc
     8641 aaaatcgaga ccatctggcc gctcgtcgag cgcatcgagc agctctcggg ccggcgctat
     8701 gccggcaacc ctgttccgtt ccgcgtgatc gccgaccatc tccgcgcgtc gaccttcgcc
     8761 atcgccgacg gcgcgacgcc ctcgaacgtc gaggcaggct acgtcgtgcg gcggctgatc
     8821 cggcgcatgg tgcgctacgg ccgcgacctg ggcattcagc gcaacttctg cgccgagctg
     8881 tcgagcgtgg tcgtcgcgct cttcgccggc gtgtatcccg aaaccgagca gaaccacgct
     8941 cgcattcagg acgagcttga ccgggaagaa accaagttca agcgcacgct cgagcgcggc
     9001 ctgcgcgagt accacaaggt cgccgggcgc acagccggcg caggcagtcg cgccatctcc
     9061 gcagccgacg ccttcaacct gttcgagacg t
//
LOCUS       DBNHXB010000319         4571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3609594, whole genome shotgun sequence.
ACCESSION   DBNHXB010000319 DBNHXB010000000
VERSION     DBNHXB010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3609594"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="AC162G_12905"
     CDS             <1..332
                     /locus_tag="AC162G_12905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR5076750.1"
                     /translation="KWRYPMAIYMTARFRVKPESLEKCQQAIREFIDYVRANEPETRL
                     YESVQETGDAMSFLHFFVFEDEAARERHSNSDGVERFTSILYPELLAPVEFTEYTLVA
                     STAKPLP"
     gene            complement(329..736)
                     /locus_tag="AC162G_12910"
     CDS             complement(329..736)
                     /locus_tag="AC162G_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR5076751.1"
                     /translation="MNEDQLRRILETYRTVAVVGLSSNPDKTSYGVAVYLKRQGYRII
                     PVNPTVDQILGEKAYPDLASIPDKVDIVQVFRRSEDVPPIVDEAIQIGAKVVWMQEGI
                     RHDAAAQKARAAGLEVVMDACMRQTHQWLIGER"
     gene            complement(886..2274)
                     /locus_tag="AC162G_12915"
     CDS             complement(886..2274)
                     /locus_tag="AC162G_12915"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5076752.1"
                     /translation="MSIEFLRRLPLLAGLSDADLDWLYQKTEPVSFPAGEILMREGDP
                     AGDLYIIVDGEFEISKRSGAQNIAIAVRAPGEVLGEMALLDRSPRSATVRALRDSRLL
                     KVPQDAFVQLLSSSSTAALAILRTVSQRLRTNEALLRQQEKMAGLGTLAAGLAHELNN
                     PAAAARRGVAQLRDTLADWERWTLALDACALSDDQIARVQKLRATLAECAAAPVDLDP
                     LARGDRESDAQAWLEDRGIDRAWELAPTLVTFGWDVDALQTLADTFGDDQLSVIAPWL
                     AVGCSVYALLDEVGRSAERISEIVGAVKSYSFLDQAPIQDVDVHQGLDNTLVILRHKL
                     KQGVRVRREYGAGLPRIEAYGGELNQVWTNIIDNAIDAMQGQGELTIRTYADDGHVVV
                     EIADSGPGIPPHIQSRIFEPFFTTKPPGVGTGLGLNIAYNVVQKHLGRISVTSQPGET
                     RFQVRLPIHLKR"
     gene            complement(2398..4053)
                     /locus_tag="AC162G_12920"
     CDS             complement(2398..4053)
                     /locus_tag="AC162G_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5076753.1"
                     /translation="MAKPVIFTIDDEPAVLNAVERDLRKQYGRDYRILKADSGITALS
                     TLEQLQRRNETVALFLVDQRMPQMAGVQFLEQARVLYPDAKRALLTAYADTEAAIDAI
                     NRAALDYYLLKPWDPPEERLYPTLDDMLDDWKAHARLPFEGIRVAGTMWSAASHQVKD
                     FLSQHQIAYQWLDVEKDEKARGLAESCTDGQLVIPTVLFPDGTALARPSIRELAEKVG
                     LQTRAAWRFYDLVIVGAGPAGLAAAVYASSDGLKCLVIERQAPGGQAGRSPKIENYLG
                     FPSGLSGGDLARRAVTQARRFGAELLSAQEAVRVRAQDIYRIVTLSDGAEISCHAVLL
                     ACGASFNILKMPGAAELTGAGVYYGAAYTEAFFYANQDVFVAGGANSAAQGALFLSRF
                     ARKVTVLIRGPAPAASQYLVDAMRQDEKIELMPDTDLIEVHGQDKLEAVVVKNSVTGE
                     VQTLRGAALFVFIGVKPQSDLVADLVLRDPKGYVLTGPDLLQDGKPPKGWPLARDPYM
                     LETSVPGIFAAGDVRFGTNHRVASATGEGAVAVATIHQYLKTL"
     gene            4471..4543
                     /locus_tag="AC162G_12925"
     tRNA            4471..4543
                     /locus_tag="AC162G_12925"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4504..4506,aa:Ala,seq:cgc)
BASE COUNT          788 a         1490 c         1446 g          847 t
ORIGIN      
        1 acaagtggag atacccgatg gcaatctaca tgactgcccg cttccgggtg aagcccgagt
       61 cgttagaaaa atgccagcag gcgatccgcg agttcatcga ctacgtccgg gcgaacgagc
      121 cggagacacg gctgtacgag tcggtgcagg agacgggtga cgcgatgagc ttcctgcact
      181 tctttgtctt cgaggacgaa gccgcgcgag agaggcacag caattcggac ggcgtcgagc
      241 gtttcacgag cattttgtac cccgaactgc tggcgccggt cgagttcaca gaatacacgc
      301 tcgtcgcctc aactgcgaag cccctgccct aacgttcccc aatcagccac tgatgcgtct
      361 gccgcatgca cgcatccatc acgacttcca ggccggcggc tctcgccttc tgcgccgcgg
      421 cgtcgtgacg gattccctcc tgcatccaca cgaccttcgc gccgatctga atcgcttcgt
      481 cgacgatcgg cggcacgtcc tccgagcgcc ggaacacctg cactatgtcc actttgtccg
      541 gaatggaggc gagatccgga tacgccttct cgccgagaat ttgatcgacg gtcggattga
      601 ccgggatgat gcggtagccc tgccgcttca ggtacaccgc cacgccgtaa ctcgtcttgt
      661 ccggattaga tgacagaccc accacggcga cggtgcggta cgtctcgagt atgcgcctga
      721 gttgatcttc gttcattcgt ttatcctcgc ttgaggtaac tgctcaaccc gcgtcattgc
      781 gagcgctttt tgcgaagcaa tcccctgtac ggacagccat gtgcgctcag ggcggcggag
      841 attgcttcgt cgcaaacggc gctcctcgca atgacggccg agtagttatc gcttgaggtg
      901 gataggcagc ctgacctgga agcgtgtctc gcccggctgc gacgtgacgg agatgcggcc
      961 caggtgcttc tgcacgacgt tgtaggcgat gttcaagccc agccccgtgc cgacgccggg
     1021 cggcttggtc gtgaagaacg gctcgaagat gcgcgattgg atgtgcggcg ggatgcccgg
     1081 cccgctgtcg gcgatctcga cgaccacgtg cccgtcgtcg gcgtacgtgc gtatcgtcag
     1141 ctcgccctga ccctgcatcg cgtcgatcgc attgtcgatg atgttggtcc agacctggtt
     1201 cagctcgccg ccgtaggcct cgatgcgcgg caggccggcg ccgtactcgc gccggacacg
     1261 cacgccctgc ttgagcttgt gccgcagaat cacgagcgtg ttatcgagac cctggtgcac
     1321 gtcgacgtcc tggatcggcg cctggtcgag aaacgagtac gacttcaccg ccccgacgat
     1381 ttccgatatg cgctccgcgc tccggccgac ttcgtcgagc aaggcgtaga ccgagcagcc
     1441 gaccgccagc cacggcgcga tcaccgacaa ctgatcgtcg ccgaaagtat cggcgagggt
     1501 ctgcagcgcg tcgacatccc agccaaacgt cacgagcgtg ggcgccagct cccaggcccg
     1561 atcgatcccg cgatcctcca gccacgcctg cgcgtcggac tcgcgatcgc cgcgcgccaa
     1621 cgggtcgagg tcgaccggcg cggctgcgca ctcggccagc gtcgcgcgca gcttctgaac
     1681 ccgggcgatc tgatcgtcgg acagcgcgca ggcgtcgagc gccagcgtcc atcgctccca
     1741 atcggcgagt gtgtccctca actgcgcgac gccgcgccgc gccgccgcag ccgggttgtt
     1801 tagctcgtgc gccaggccgg cggccagcgt gcccaggccg gccatcttct cttgctgccg
     1861 cagcagcgcc tcgttcgtcc gcagacgctg ggatacggtg cgcaggatgg cgagcgccgc
     1921 cgtcgagctg ctcgacagaa gctgcacgaa agcatcctgc ggcaccttaa gcagacggct
     1981 gtcgcgcagc gcgcgcactg tcgccgagcg aggcgagcgg tcgagcaggg ccatctcgcc
     2041 gagcacttca cctggcgcgc gcacggcgat ggcgatgttc tgcgctcccg accgcttgct
     2101 gatctcgaac tcgccatcca cgatgatgta caaatcgccg gcgggatcgc cttctcgcat
     2161 caggatttcg ccggccggga acgaaacagg ctccgtcttc tgatagagcc agtcgaggtc
     2221 ggcgtcggac aggccggcga gcagcggcaa ccgccgcagg aactcaatgc tcatatacca
     2281 agtctttgga cgcggattta cgcggacgaa cacggataga ttacaaatcc gcgaaatccg
     2341 cgttcatccg cgtcctaaaa atcttctcgc tctttgcgcc ttcgcggtgc aaggtcttca
     2401 cagcgtcttc aagtattgat ggatcgtcgc caccgcgacg gccccttcgc cggttgccga
     2461 ggcgacgcgg tgattcgtgc cgaagcggac gtcgccggcg gcgaagatgc cgggcacgct
     2521 ggtctccagc atgtacgggt cgcgcgcgag tggccagcct ttgggtggct tgccgtcctg
     2581 cagcaggtcc ggcccggtaa gcacgtagcc tttcgggtcg cgtagcacca ggtcggcgac
     2641 caggtcgctc tgcggcttca cgccgatgaa cacgaacagc gccgcgccgc gcagcgtctg
     2701 cacttcgccg gtcacgctgt ttttgacgac gacggcttcg agcttgtcct ggccgtggac
     2761 ctcgatgagg tcggtatcag gcatcagctc gatcttctcg tcctgccgca tcgcatcgac
     2821 gagatactgg gaggccgccg gcgccgggcc gcggatcagc acagtcacct tgcgcgcgaa
     2881 gcggctcagg aacagcgcgc cctgcgccgc cgagttcgcc ccgccggcga cgaacacgtc
     2941 ttgattggcg tagaagaacg cttcggtata ggccgcgccg taatagacac ccgcgccggt
     3001 caactccgcc gcgcccggca ttttcaggat attgaacgac gcgccgcacg ccagcagcac
     3061 ggcgtgacaa ctgatctccg cgccgtcgga gagcgtgacg atgcgataaa tgtcctgcgc
     3121 gcgcacgcgc accgcctcct gcgccgacaa gagctcggcc ccgaatcgac gcgcctgcgt
     3181 taccgcgcgc cgcgcgagat cgccgcccga cagcccggaa gggaatccga ggtagttctc
     3241 gatcttgggg ctgcgtccgg cctgcccgcc gggcgcctgc cgctcgatga ccaggcactt
     3301 cagcccgtcc gagctggcgt acacggccgc cgccaggccg gccgggccgg caccgacaat
     3361 gacgagatcg tagaaacgcc aggcggcgcg cgtctgcagt ccgactttct cggccagctc
     3421 ccgaatcgac ggccgcgcca acgccgtgcc gtcggggaag agcacggtgg gaatgacgag
     3481 ctggccgtcc gtgcacgatt cggccagccc gcgcgccttc tcgtccttct ccacgtcgag
     3541 ccactggtag gcgatctggt gctgcgacag aaagtctttg acctggtgcg aggcggccga
     3601 ccacatcgtg ccggccacgc gaatgccctc gaagggcagg cgcgcgtgcg ctttccagtc
     3661 gtcgagcata tcgtcgagcg tgggatatag tcgttcttca ggcgggtccc acggcttcag
     3721 gagataatag tcgagcgcgg cgcggttgat cgcgtcgatc gccgcctccg tgtcggcgta
     3781 ggcggtgagc aaggcacgtt tcgcgtcggg atacagaaca cgcgcctgct cgaggaactg
     3841 cacaccggcc atttgcggca tgcgctggtc cacgagaaac agcgccaccg tctcgttgcg
     3901 ccgctgcaac tgttcgagtg tgctcagggc tgtgatgccg gaatcggcct tcaagatgcg
     3961 gtagtcgcgc ccgtattgct tccgcaggtc gcgctcgacg gcattcagca cggccggttc
     4021 gtcgtcgatg gtgaagatga ctggtttcgc cattctgctc cctaaaagat ggttggctca
     4081 acttgctggg cttatcgaga gtatgcgcca aacccgccgg gctgtcaacc cggtttattc
     4141 gcggtttgta actgctcagc cacttgtagc gtgtcattcc gaacgcagtg aggaatgtct
     4201 actttgtcga gtgtcaggtg tcaggtgtcg gacagtgtca ttccgaacgc agtgaggaat
     4261 ctctcgtccg accggcgagc gcggcgcgcc ggggtagaga tgcctcgtcg tgcctcctcg
     4321 gcatgacacg cctggcgcgg atcgtgtggc acacctatgt gcgccatacc ggatggcgtt
     4381 ctttgccgtg taggactcgc gatgacgcct gagtagttac cacggtttct tgacagtgat
     4441 tgtcaagtcg ggtagaatcc gtcacgtcga gggcctgtgg ctcagctggg agagcgctgc
     4501 aatcgcactg cagaggtcga gggttcgaat ccctccaggt ccattgggca gtgggcagta
     4561 aacagttatc a
//
LOCUS       DBNHXB010000320         9128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3609699, whole genome shotgun sequence.
ACCESSION   DBNHXB010000320 DBNHXB010000000
VERSION     DBNHXB010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9128
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3609699"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(448..1566)
                     /locus_tag="AC162G_12930"
     CDS             complement(448..1566)
                     /locus_tag="AC162G_12930"
                     /inference="COORDINATES: protein motif:HMM:NF024772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5076754.1"
                     /translation="MKPRMLRNSPRLLVGWLASLIILSLIVASCAAPPVAPQPVVETV
                     VVTQAVPVPVTQVVEITPVPLTKVDACFSAASGTMAVAWYAYENDLFEKYGLDVNLTF
                     IASGSKAATAMIAGNVEFCQIAGNAVTNAVVAGGGLVMIGGLFNTYTYSLMASPDIAS
                     VDDLRGKTLGISRPGSSSDAALRAALKSLGLDPDTDVTIVTIGSEPERIAALEAGAIA
                     GTVINVPESSEAKAKGFKELADLSTLGIPFQHTGIATSRAYIESNREVVVNFMKAIAE
                     AIAMMKQDKEGTIAVIADYLLLDPEESEASLNEAYDVLVVNFLPQAPYPTLDGIQTLL
                     TAAAEEDPNAANFKPEDVADLSIVKELEDSGFIASLYK"
     gene            complement(1780..2652)
                     /locus_tag="AC162G_12935"
     CDS             complement(1780..2652)
                     /locus_tag="AC162G_12935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076755.1"
                     /translation="MADVTETIGVEPALSEVEGSSAGEAEAPASRPTGLRAFYRKHEN
                     TILPLTTVVVFFAAWELVAGLGLIRPLLISSPSRILKAAVWLFEHGFWNDILVSANEF
                     GVGFALSVVIGIPLGILMGWYRRLNVMFDPFISALYAMPRIALLPILILWLGIGIESK
                     IAVVFLGAFFPMLVSTMAGMKTIDESLLKCARSFGARDRQVFTTLALPGSVPFIITGM
                     RLGMGRGLVGVVVGELVASNAGVGYVMSLAGATFQTDKVYVGILILALTGYVLVEVLK
                     WLEARFESWRPERG"
     gene            complement(2640..3464)
                     /locus_tag="AC162G_12940"
     CDS             complement(2640..3464)
                     /locus_tag="AC162G_12940"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076756.1"
                     /translation="MAKLEVQNVHIEYLIKRTGQRIMAVGGVSFAVQGGQFVTIVGPS
                     GCGKTTLLNAIDGLVPVKSGRILLDGQEIRGPGHERAMVFQSPALMPWRTVMRNVTYG
                     LELRGSDPAEARATAQRFIDVVGLHGFEESFPRELSGGMQQRANLARALTAGPELLLL
                     DEPLAALDAQTREYMQFELQRIWWETRKTAVYVTHQISEAIYLGDQVIVVSARPGRVK
                     EVVPVDLPRPRSLRVKRQPGFIELEEHIWNLIQEEAAKTGMLVHEEERVEVRSWQT"
     gene            complement(3451..4893)
                     /locus_tag="AC162G_12945"
     CDS             complement(3451..4893)
                     /locus_tag="AC162G_12945"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR5076757.1"
                     /translation="MPYTDLRDWLRQVDEMGELRCVNEATLDEDAGRIAEMLQHTDDA
                     PTALLGHFEGYPPGFRILVNALSKRTRIALTLGLPNDIGPWELMDRFLEMNTAVKPSP
                     VQQVGDGPILENVMTGDEVDVTIFPTPLWHEKDGGRYIGTGCVNVIKDPDSDWVNLGT
                     YRGMIQDKTKVGIYISPGKHGRLFRDKYFERGQPMPVAMVVGGDPLLFMASSLELPAG
                     MNEYEWAGAIRGEPFRVIRGKVTGLPIPAEAEIVLEGFVDPVARLPEGPFGEWTGYYA
                     SSQRPEPFIDEKAIYYRNDPIILGIPPQKPPYEAHRYRVYLRSALLKQALLQAGVPDV
                     TAAWCHGVGGCRLLNVVAIKQRYPGHARQAGHVAAMCRAGAYLGRIVIVVDDDIDVTD
                     LGDVMWAVCTRADPERSLDIIKRAWSGPLDPAIRPGEKGFNSRLIVDACRPWEWREEF
                     PPAIGPSPEVKRATREKWNHLLREVASGKA"
     gene            complement(4981..6261)
                     /locus_tag="AC162G_12950"
     CDS             complement(4981..6261)
                     /locus_tag="AC162G_12950"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5076758.1"
                     /translation="MQSLIQVYLVIFLITCSEGTIHVIFPPFLESLAYAVSQIGVLSS
                     LFAVLQLAARLPAGAFYAPQRARVILVGGLALLGLTALGFPTVQGLVPLVALIGLHGF
                     AFGTITTVALALCIDTRPKDYPSGAMMGWYTSAIAAGYAVGQPIGGYLADEFGFPMGF
                     GALAGFSLLAILILLSLPRLGQSHQKTGPIPSAHPATAGHAGHTRHQWSLRFDPRRLP
                     AEVLLATLIVFFVNMMFRSLHTFFPLYALAAGITLTQIGLLRSMLSLAAAVVRPFSGK
                     LFQVIHYRRVNHMAMIGAAASVLLSPLMVGSMTLLAVLHTLMGLSRGLTRVTSATMLA
                     ESNATEAPPLSPPSDGGDDRGGRNARIGIWSGVYNAGLDLGSITGPAVGGILAGWIGI
                     PNMLGTIAIGVLGTYVFISLLARRRRVPVAAAVD"
     gene            complement(6371..8119)
                     /locus_tag="AC162G_12955"
     CDS             complement(6371..8119)
                     /locus_tag="AC162G_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR5076759.1"
                     /translation="MPTIRDLQGRSGNDQGASREIQGEGTGADLVVAYLNAEGVKFVF
                     GVIGSSILDLTDALYRAPSLRYIPTQHEQAAAFMADGYARVTGQPGVCIATVGPGATN
                     LTSGIAQAFVESSPVIALTGDIHTRHYGKGSSNFHEIDQQALYRPITKMSWRVEHPER
                     LGEFLRMAFRTALSGRRGPVYLGLPRDVLKARMPIEAPPPETYRVLDQRMHPDPTQVS
                     LAADLLARAERPVMILGGGLRWSPGALEAAVSLAEAWHLPIVHTLKGLAPEDHPLVFG
                     LIGSTNSPVATQAVQESDLILAVAHTFSQVATNSYGYKTIPRHSRIVHIDLDALEIGK
                     NYPVVAGLVGDAAAALNDLRLALPPAPQSNRRAWLATLERARVLFWEQLAAMADNDAR
                     PISRMRLLRDLQTVLDRDAIISAEAGATHQWYRFGYCATQPLLEPGGYSIMGSAFCMC
                     LAAKLAYPERQAVATLGDGAFMMIMPEVATAVAQNIPILAIVCHNNVYGNVQSKQYLN
                     FDGRRIGTDLYIPDLAAVARDLGAYGERVEAPEAIIPALERALGSGKPAVLDVIIEAA
                     PETLEPPTKVRVADRY"
     gene            complement(8137..>9128)
                     /locus_tag="AC162G_12960"
     CDS             complement(8137..>9128)
                     /locus_tag="AC162G_12960"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076760.1"
                     /translation="TTTLRTIAGLERPEAGEIRINGRVVCSPAQGIFVPPNERDISMV
                     FQSYAIWPHMTVFENVAFPLTVGKRKPGKQEIARRVDKALSLVQLDGLKNRPAPNLSG
                     GQQQRLALARALVREPKVLLLDEPLSNLDAKLREQMRIELRELQRRLQVTTIYVTHDQ
                     AEALAMSNLVAVMDRGRIVQLGRPRDIYDLPATKFTADFVGSTNFVPGTVIGDPVEDG
                     ARLVETQHGNLLCHVPADIQKGETVLISIRPQNVLVSREPAPNAHNVFEGKVRVSVFL
                     GEYVDCQIEVGNDVLRVHLHPRLRISRGEEVFVHLPRDLCTVVPNESRPLDQA"
BASE COUNT         1714 a         2921 c         2876 g         1617 t
ORIGIN      
        1 agaaactggt acgggccttg gcgggcaaac cttttcgtgt gcattcgttg gtggcattca
       61 gcataccgcc tttaccatag gatcaggccg cccaatgaga caactgtagt taggcgcacc
      121 cgcgataccc acgcgaagac ctggtctcta tcggcagtat gatcatcgac aagcgccatc
      181 gacgacgggg cttgggccgc taggccattg cagaagttga ggagtggccc cgggcaaaat
      241 atcccggact cacgctcccc ttctatgcac gaacagcggc gggccaatga ccctgccccg
      301 gccagccgat ttcccactcc acctgccccc ggcaaggggg ttcggtcggg gatccttcga
      361 cccttcgact atgctcaggg cgggcttcgc tcaggacagg cttcgcaggg cagggtccat
      421 ctggatcgct gaatttggga gcgcgcctta cttgtacagg ctggcgatga agccgctgtc
      481 ctctagctcc ttcacaatgc tcaggtcggc gacgtcctcc ggcttgaagt tggctgcgtt
      541 tggatcttct tccgcggcag ccgtcagcag ggtctgaatg ccgtccagcg tggggtaggg
      601 cgcctggggc aggaagttca cgaccagcac gtcgtaggct tcgttcagcg acgcctcact
      661 ctcctcggga tcgagcagca ggtagtcggc gatgaccgct atcgtgccct ctttatcttg
      721 cttcatcatg gcgatggctt cggcgatggc cttcatgaag ttgacgacga cctcgcggtt
      781 tgactcgatg taggcccggc tggtggcgat gcccgtgtgc tggaagggaa tgcccagcgt
      841 agacaggtcc gccagttcct tgaagccctt cgccttggcc tcgctcgatt cgggcacgtt
      901 gatgaccgtc cccgcgatcg cgcccgcttc caacgccgcg atgcgctcag gctccgagcc
      961 gatggtgaca atggtcacgt ccgtatcggg atccaggccc aggctcttca gggccgcgcg
     1021 caaggcggca tccgacgagg agccaggccg gctgataccc agggtcttgc ccctgaggtc
     1081 gtcgacgctg gcgatgtcgg ggctcgccat gagcgagtag gtgtaggtat tgaacaggcc
     1141 cccgatcatc accaggcccc cgccggccac cacggcgttg gtcaccgcat tgccggcgat
     1201 ctggcagaac tccacgttgc cggcgatcat ggccgtagcc gccttggagc cgctggcgat
     1261 gaaggtcagg ttcacgtcca gaccgtattt ctcgaacaag tcgttttcat aggcgtacca
     1321 ggccacggcc atcgtgccgc tggccgcgct gaagcacgcg tccactttgg tgagcgggac
     1381 gggcgtgatt tccacgacct gggtgacggg gaccggcacc gcttgggtga cgacgacagt
     1441 ttcgacgacc ggctgcggcg ccaccggcgg cgccgcgcac gaggcgacga tcagactcag
     1501 gatgatcagg ctggccagcc atcccaccaa aagacgggga ctgttccgta acattcgtgg
     1561 tttcatttga ctcctcctcc aagttgctca aggttgccgc ccgcacggca ggcggcgatg
     1621 gtccagacgc gtagcacgac acggattctg ggcacggatt acacggattg gcaagtagtg
     1681 tgacctcctt tcgcaggcgc cccgccgacg ggtgacgggt gacgaatgac gagggctcct
     1741 cgccacgcgt cactcgtcac gcttcacgtt tcggggcatc taccctcgct ctggccgcca
     1801 cgattcgaag cgcgcttcga gccacttcaa cacttcgacg agaacgtagc cggtcagggc
     1861 cagaatcaga atgcccacat aaaccttgtc cgtctgaaag gttgccccgg ccaggctcat
     1921 cacgtagccc acgccggcgt tggacgccac cagttcgccg acgacaacgc ccaccaaccc
     1981 ccggcccatt cccaaccgca tgccggtaat gatgaacggc acggaaccgg gcagggccag
     2041 ggtcgtgaag acctggcggt cgcgggcgcc aaaggaacgg gcgcatttca gcagcgactc
     2101 gtcgatcgtt ttcatcccgg ccatggtgct gaccagcata gggaaaaaag cacccaggaa
     2161 gacgacggcg atcttggatt cgatgccaat gcccagccac aggatcaaga tcggcaacaa
     2221 cgcgatgcgc ggcatggcgt atagggccga gatgaaggga tcgaacatga cgttcagccg
     2281 ccgataccag cccatcagga tccccagcgg gatgccgatg acgacggaca aggcgaagcc
     2341 gactccgaac tcgttggcgc tgacgaggat gtcgttccaa aagccatgct cgaacagcca
     2401 aacggctgcc ttgaggatgc ggctgggcga gctgatgagc aggggcctga tcaggccgag
     2461 tccggccacg agttcccagg cagcaaagaa gacgacgacg gtggtcaggg gcaagatcgt
     2521 gttctcgtgc tttcgataga atgcccgcaa tcctgtcggg cggctcgccg gcgcttccgc
     2581 ctcgccggcg ctcgaccctt cgacttcgct cagggcaggt tcgacgccga tcgtttcggt
     2641 cacgtctgcc atgatcgcac ctccactctc tcttcttcat gaaccagcat gccggtcttg
     2701 gccgcctctt cctgaatcag gttccagata tgttcttcca gttcgatgaa ccccggctgc
     2761 cgcttgacgc gcagcgagcg cgggcgcggc aggtccaccg gcaccacttc cttgacccgg
     2821 ccgggccggg cggacaccac aatgacctga tcgccgaggt agatggcctc gctgatttga
     2881 tgggtcacgt acaccgccgt cttgcgggtc tcccaccaga tgcgctggag ttcgaactgc
     2941 atgtattcgc gcgtctgcgc gtccaaagcg gccagcggct cgtccagcag caacagttcg
     3001 ggaccggccg tcaaggcccg agccagattg gcgcgctgct gcatcccgcc ggacaactcg
     3061 cggggaaagg attcctcaaa accgtgcaag ccgaccacgt ctatgaagcg ctgcgccgtg
     3121 gcgcgggctt ccgcagggtc gctgccgcgc aattccaggc cgtaagtcac gttgcgcatg
     3181 accgtccgcc agggcatcag ggctggagat tggaagacca tcgcccgctc gtggcccggg
     3241 cctctgatct cctggccgtc cagcaagatg cgccccgatt tcaccggcac cagtccgtca
     3301 atggcgttca ggagtgtggt cttgccgcaa ccactaggcc cgacgatcgt cacaaactgg
     3361 cctccctgga cggcaaagga gaccccgccg acggccataa tgcgctggcc ggtgcgctta
     3421 atgaggtatt caatgtggac gttttgaacc tcaagctttg ccactggcaa cctcccgcag
     3481 tagatggttc cacttctcgc gcgtggcgcg cttgacttcc gggcttggcc cgatggcagg
     3541 cggaaactcc tcccgccatt cccagggccg gcaggcgtcg acgatcagcc gcgagttgaa
     3601 gcctttctcg ccgggtcgaa tggcagggtc gagcgggcca ctccaggcgc gcttgatgat
     3661 gtcaagcgag cgctcgggat cggcgcgagt gcacaccgcc cacatcacgt cgcccaggtc
     3721 ggtcacgtcg atgtcgtcgt ccacgacgat cacgatgcgt cccaggtagg ccccggcacg
     3781 gcacatggcg gccacgtgcc cggcttggcg cgcgtggccg ggataacgct gcttgatggc
     3841 gacgacgttg agcaggcggc agccgcccac cccgtgacac caggcagccg tcacatctgg
     3901 cacgccggcc tggagcaatg cctgcttaag gagggccgag cgcaggtaca cccggtaacg
     3961 gtgggcctcg tagggcggct tctgcggtgg gatgcccagg atgatcggat cgttgcgata
     4021 gtagatagcc ttctcgtcga tgaacggctc gggccgctgt gaactggcat aatatccggt
     4081 ccattcaccg aacggccctt ccggtaaccg cgcgacagga tcgacaaagc cttcgagcac
     4141 gatttcggcc tcggccggga tcggcagccc cgtgaccttt ccccggatca cccggaaagg
     4201 ctcaccccgg atcgcgccgg cccactcgta ctcgttcatc ccggcaggca gctccagcga
     4261 gctggccatg aacagcagcg ggtctccgcc gacgaccatc gccacaggca tcggctggcc
     4321 ccgctcgaaa tacttgtccc ggaagagacg cccgtgcttg cccggcgaga tgtagatgcc
     4381 cactttcgtc ttgtcctgga tcatgccacg ataggtgccc aggttgaccc aatcgctatc
     4441 cgggtctttg atcacgttga cgcagccggt gccgatgtag cgcccgccgt ccttctcgtg
     4501 ccacagcggg gtgggaaaga tcgtcacgtc cacctcgtcg ccggtcatca cgttctccag
     4561 gatcggccca tcgccaacct gttgaaccgg ggatggcttg accgccgtgt tcatttccag
     4621 aaagcgatcc atcagttccc aggggccaat atcgttcggc aggccgaggg tcaacgcgat
     4681 gcgcgtgcgc ttactgaggg cgttgacgag gatgcggaat ccaggcgggt aaccttcaaa
     4741 atggcccaac agcgcggtcg gcgcatcgtc ggtgtgctgc agcatttcgg cgatgcgccc
     4801 cgcatcctcg tcgagcgtgg cctcgttcac gcagcgcagt tcacccatct catccacttg
     4861 tcgcagccaa tcgcgtaagt ccgtgtatgg catggctatg ccctccgcct caaggtaagg
     4921 taacgtgaag cgtaaaacgt gaagcgtgga gtgtggaggg acgtcgcacc ttaacaggca
     4981 ttagtctacc gccgccgcca cgggcacccg ccggcggcgc gccagcaggc tgatgaacac
     5041 gtacgtcccc aatacgccga tcgcgatagt ccccagcata ttgggaatcc cgatccagcc
     5101 ggccaggatg ccgcccaccg ccgggccggt aatcgaaccc agatcgaggc cggcgttata
     5161 cacgccggac cagattccga tgcgggcatt gcgacccccc ctgtcatccc ccccgtcaga
     5221 cgggggggat agaggggggg cctccgtcgc attactttcg gccagcatcg tggcgctggt
     5281 cacacgggtc aggccgcggc tcaggcccat gagcgtgtgg agcaccgcca gcagcgtcat
     5341 tgagccgacc atgagcggcg acaagaggac ggacgccgcc gccccgatca tggccatatg
     5401 gttgacccgg cgataatgaa tcacctgaaa gagcttgccg gagaaggggc gcacgacggc
     5461 tgcggctagc gagagcatgc tgcgcaagag accgatctgg gtcagagtaa tgccagccgc
     5521 cagcgcgtag agcggaaaaa acgtgtgcag cgagcgaaac atcatattga cgaagaagac
     5581 gatcagcgtc gccagcagca cctcggccgg gagtcgccgg ggatcgaatc gcaatgacca
     5641 ctgatggcgt gtgtgcccgg cgtgcccggc ggtcgccggg tgggcagacg gaataggccc
     5701 cgtcttttgg tgggattgtc ccagcctggg caggctcaag aggatcagga tcgccaacaa
     5761 ggaaaacccg gccagggcgc cgaaccccat cggaaagcca aactcatccg caaggtaacc
     5821 gccgatgggt tgacccacgg catagccggc ggcaatggca gaggtgtacc agcccatcat
     5881 cgcgccgctg gggtagtctt tcggcctggt gtcgatacac agcgccagcg ccaccgtggt
     5941 gatcgtgcca aaggcaaagc cgtgcaaccc gatcagagcg accagcggca cgaggccttg
     6001 aacagtcggg aagcccagtg cggtcaagcc caggagcgcc aggcctccca ccaggatgac
     6061 tcgggcgcgc tggggcgcat agaaggctcc ggctggcagc cgcgcggcga gttgaagcac
     6121 ggcgaacaag gaagacagca cgccgatctg gctgaccgca taggcgagcg actcgagaaa
     6181 gggtgggaag atcacgtgga tcgtcccttc ggaacaggta atcaggaaga taaccagata
     6241 gacctggatc aggctctgca tgtgtgccgt tgccggtgat cagtcaccag tcatcagtga
     6301 tcagtaatca gtcatcagtg atccgtcgcc ggtgatcagt ggtcactggt aactgaccac
     6361 tgatcactgg tcagtaacgg tccgccacac gaactttggt cggcggctcc agcgtctcgg
     6421 gcgccgcctc gatgatcacg tccagcacgg ccggcttacc gctgcccagc gcgcgctcca
     6481 gcgcggggat gatagcctcg ggtgcttcca ctcgttcgcc gtacgcgccg agatcgcgcg
     6541 ctaccgccgc caggtccggg atgtacaggt ccgtgccgat gcgccgcccg tcgaagttca
     6601 gatattgttt gctctgcacg ttgccataca cgttgttgtg acacacgatc gcgaggatcg
     6661 ggatattctg cgcgacggcg gtcgccacct cgggcatgat catcatgaac gcgccgtcac
     6721 ccagcgtcgc cacggcctgg cgttccgggt aagccagctt ggccgccagg cacatgcaaa
     6781 aggcgctgcc catgatgctg tatccgccag gctcgagcaa aggctgcgtc gcgcaatagc
     6841 caaagcgata ccactggtgg gtggcgccgg cctcggcgct gatgatcgcg tcacgatcca
     6901 gcacggtctg caggtcgcgc agcaaacgca ttcggctgat cggccgggcg tcgttgtcgg
     6961 ccatggcggc caactgttcc cagaacagca ccctcgcccg ttccaacgtc gccagccagg
     7021 cccgccggtt ggattgaggc gccggaggca gcgcaagcct cagatcgttc agcgcggcgg
     7081 cggcgtctcc gaccagcccg gccaccaccg ggtagttctt gccgatctcc agcgcatcca
     7141 ggtcaatgtg cacaatgcgg gaatggcgcg gaatcgtctt gtaaccgtaa ctattggtcg
     7201 ccacctggct gaaggtgtgc gccaccgcca agatcaaatc ggactcctgc acggcctgcg
     7261 tggcgaccgg gctgttggta gagccgatca atccgaagac gagcggatgg tcttccggag
     7321 ccaggccctt gagcgtatgg acgatgggca ggtgccaggc ctcggccagg gatacagccg
     7381 cttccaacgc gccgggcgac caacgcaatc cgccgcccag gatcatgacc ggtcgctcgg
     7441 cccgggccaa cagatctgcg gcgaggctta cctgcgtggg gtcagggtgc atgcgctggt
     7501 cgagcacgcg atacgtctcg ggcggtggcg cctcgatcgg catgcgggcc ttgagcacgt
     7561 cgcgcgggag gcccaggtat accgggccgc gccgcccgct gagggcggtg cgaaacgcca
     7621 tgcgcaggaa ctcccccagc cgctcggggt gctcgacccg ccagctcatt ttggtgatgg
     7681 ggcgatacag cgcctgctga tcgatctcgt gaaaattgga cgagcctttg ccgtagtgac
     7741 gggtgtggat gtcgccggtc agcgcaatga cgggcgagga ttcgacgaaa gcctgggcga
     7801 tgccggaagt caggttagtc gcgcccggcc cgacggtggc aatgcacacg cctggttggc
     7861 cggtgacgcg ggcgtagccg tcggccatga aggccgccgc ctgctcgtgc tgagttggaa
     7921 tgtagcgaag tgagggtgcg cggtaaagag cgtcggtcag atcgaggatg gaactaccga
     7981 tgacgccgaa gacaaacttg actccttcgg cgttgagata agccacgacc agatcggcgc
     8041 ccgtgccctc gccctgaatt tcccggctcg cgccctgatc gtttccggaa cggccttgaa
     8101 gatcacgtat ggtcggcatc cggaggcgcc tccacctcag gcttggtcca atggccggct
     8161 ttcattggga acaaccgtgc acaggtcgcg cggcagatgg acaaagacct cttcaccccg
     8221 gctgatcctc aagcgtggat gcagatgcac gcgaagcaca tcgttgccga cctcgatctg
     8281 gcaatcgacg tactcgccga gaaaaaccga aacacgcact ttgccctcga agacattgtg
     8341 cgcgttcggc gccggctcgc gggagaccag cacgttttga ggacggatcg agatcaagac
     8401 cgtctcgcct ttctgaatat cggccggcac gtggcacagc aagttcccat gctgggtttc
     8461 gaccagccgg gcgccatcct cgaccggatc gccgatgact gtgcctggga caaagttcgt
     8521 ggagccgaca aaatcggctg tgaacttggt tgcgggcaga tcgtagatgt ctctgggtct
     8581 gcccaattgc acgatccggc cccggtccat caccgccacc aggttcgaca tcgccagcgc
     8641 ttcggcttga tcgtgcgtga cgtagatggt cgtcacctgg agccggcgct ggagctcgcg
     8701 cagctcgatg cgcatttgtt cccggagctt ggcatccaaa ttgctcagtg gttcgtccag
     8761 cagcagcact ttcggctcgc gcacgagggc gcgcgccagc gccaggcgct gctgctgccc
     8821 gccgctgagg ttgggcgcag gccgattctt caagccatct aactgcacca ggctcaacgc
     8881 cttgtccacc cgcctggcaa tctcctgttt gccgggtttg cgcttgccga ccgtcaacgg
     8941 aaaggccacg ttctcgaaga ccgtcatgtg cggccagatg gcatacgact gaaacaccat
     9001 gctgatgtcg cgctcgttcg gcggcacgaa gatgccctgc gccggcgagc agacgacccg
     9061 cccgttgatg cgaatttcgc cggcttcggg gcgctccagc ccggcaatcg tgcgcagcgt
     9121 ggtcgtct
//
LOCUS       DBNHXB010000321         5730 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1092707, whole genome shotgun sequence.
ACCESSION   DBNHXB010000321 DBNHXB010000000
VERSION     DBNHXB010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1092707"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="AC162G_12965"
     CDS             <1..204
                     /locus_tag="AC162G_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076761.1"
                     /translation="LKVRNAKRKLTSQPRYPTELRLDIEIDQGYPLTTAQDIALREML
                     KEDKVTVQEAAGRLVALRPKPKA"
     gene            311..1594
                     /locus_tag="AC162G_12970"
     CDS             311..1594
                     /locus_tag="AC162G_12970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5076762.1"
                     /translation="MLVKETPYWWDTAPDLPGHADRPLPQRVDVAVVGSGSTGLSAAL
                     HLARSGASVAVLEKETIGWGASSRNGGQVLTGLKVGVETLVSRYGLPLAKHLYDASQA
                     ALVFVEDLIAAEQIDCEYGRCGNLYAASKPKHFEGFKRTQELLSREFATHHELVPRAE
                     QRREIGSEAYFGLMVEPDNSSLHPAKYVRGLAKAAERAGVGLHEKTPVLSFERNGSSF
                     AVKTSGGTLATEALFLATNGYTDAAAPALRRRVVPIGSYIIATEPLRADVAARLLPNR
                     RVVSDSKNFLHYYRLSADNRLLFGGRAAFVPPTGDSNRKSAEILRRDMLEVFPELADA
                     SVEYVWSGNVCFTMDMLPHAGRFDDGVHYAAGYGGHGVAMATYLGAKMADVILGKDDR
                     NPFRDLRFRAIPLYDGRPWFLPLPALWFKFLDWIR"
     gene            complement(1655..2515)
                     /locus_tag="AC162G_12975"
     CDS             complement(1655..2515)
                     /locus_tag="AC162G_12975"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR5076763.1"
                     /translation="MTRLGQVRPITPHIPDPAQDAVIAVVLAAGKGTRMKSDLPKVLH
                     TLHGQSLIQHVVERVRRVGIRDIIVVVGYQREQVMAHLGASVRYAIQEEQLGTAHAVM
                     MARPYLSNFTGRVLIAYGDMPLINPDTMKRLIDRCQGDVKASMLTIILENPPDFGRVV
                     RGPDGRVMRIIEVQDADPETLALREINVGMYCFECRALLAAFDRLSNNNAQKEYYLTD
                     AIEAIASTGGRIETIVAPTVEETLGINEPGNLRFAESLKHLDYAERMYPVIYASLAAS
                     RRSWQEGEIS"
     gene            complement(2516..3235)
                     /locus_tag="AC162G_12980"
     CDS             complement(2516..3235)
                     /locus_tag="AC162G_12980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5076764.1"
                     /translation="MRIGIGHLYGLAGSGSAIYVARLAETLASRGHYVALLCYEEHPE
                     DYWFVDAASTGRGHDVVTRFQRQPDPACWLHQLSGLAPVAYPRVEAPDRPLFTQLTDH
                     ELESYLDELTEQLVELVRRHRLEMLHVNHAVPLPAVAARVKRRTSVPYVVAVHGSMIE
                     FVVNRDDRYQPLAYTGLAAANRVIVLNRDVRTRTEALCPIAHLTEIPVGVNTEIFSPN
                     ASPERLPQLQGLLADLTGQTM"
     gene            complement(3247..3666)
                     /locus_tag="AC162G_12985"
     CDS             complement(3247..3666)
                     /locus_tag="AC162G_12985"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5076765.1"
                     /translation="MDISATHRYEVRGGNEIVGRVRAWAVETAAGLGADERATADITL
                     AVSEAVTNVVRYAYADRQECHLILTAKRIGERIVFRLRDYGDKFDPTHVRPPNMDGEL
                     TVGGYGIFLMQHVMDDVRYVTSHRAGTELFMMRRRHR"
     gene            complement(3732..4112)
                     /locus_tag="AC162G_12990"
     CDS             complement(3732..4112)
                     /locus_tag="AC162G_12990"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR5076766.1"
                     /translation="MFSTTLHDIVSREHIQVETLEFGTIVVVAVGGDVDTTTVADLNA
                     VFDDLVGRGVRNYVIDLGRVSFMDSSGLAALARLYKRIRIGHGDVRLCNLPPELRRVV
                     ELTRFDRVFDIFDERAAAVASFAE"
     gene            complement(4258..5019)
                     /gene="surE"
                     /locus_tag="AC162G_12995"
     CDS             complement(4258..5019)
                     /gene="surE"
                     /locus_tag="AC162G_12995"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR5076767.1"
                     /translation="MNILVTNDDGYAAPGLLALKTALSRVGNVVVVAPEHNYSAAGHV
                     KTMHKPLRIGEVHLRDGSTAYATTGAPSDCVALVLLGALDFKPDLLLSGINLNCNLGH
                     DVTYSGTVTAAMEGTIGGVPSIAVSAASAEAYPAAAAFATRLAAQAFERSLPRDVLLN
                     VNVPAPPIYGVRITRMGKRLYRDELIVRQDPRGRPYYWIGGERPSGVPDEGTDIWAID
                     NGYISVTPIQLDLTAHSWLETLSAWDLKAEAWQSA"
     gene            5295..>5730
                     /locus_tag="AC162G_13000"
     CDS             5295..>5730
                     /locus_tag="AC162G_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR5076768.1"
                     /translation="MVSPEPIAPADGAKIAIQNGKLRVPDYPIIPFIEGDGTGPDIWR
                     ASVRVFDAAVQKAYGGKRQIKWMEVYAGEKSFTQFSTWLPEETVEAFREYLVGIKGPL
                     TTPVGGGIRSLNVALRQLLDLYVCLRPVRWFKGVPSPVRRPER"
BASE COUNT         1045 a         1765 c         1860 g         1060 t
ORIGIN      
        1 ctcaaagtca ggaacgccaa gcgcaagctg acttcgcagc cgcgctaccc gaccgagctg
       61 cgtctcgaca ttgagatcga tcaaggttac ccgctcacga cggctcagga catcgcgctc
      121 agggaaatgc tgaaagagga caaggtgacg gttcaagagg ccgccggccg gttggttgcc
      181 ctgcgcccga agcccaaagc atagaggcag gaccgaaggc gaggtttatg gcgcgcaact
      241 tcggcggcgc cttctcgccc atcactctaa ctctaactct aactctgact tcctctccac
      301 ccaggcaaac atgctggtta aagagacacc ttactggtgg gataccgctc ccgatctgcc
      361 tggtcacgcc gatcggccgc tcccgcagcg cgtcgacgtg gcggtagtcg gcagcggctc
      421 caccggtctg tcggcggcgc tccacttggc gcgctccggc gcgtcggtcg ccgtcctcga
      481 aaaggaaacg atcgggtggg gcgcaagctc gcgcaacggc gggcaggtgc tgaccggcct
      541 gaaggtcggc gtcgagacgc tggtctcgcg ctatggcctt ccactcgcca aacacttgta
      601 cgacgcctcg caggctgcgc tcgtgttcgt cgaggacctc atcgcggccg agcagatcga
      661 ctgcgagtat ggccgatgcg gcaacctcta tgccgcgagc aagccgaagc acttcgaggg
      721 gttcaagcgc acgcaagagc ttctctcgcg cgaatttgcc acccaccacg agctggtgcc
      781 gcgcgccgag cagcggcgcg aaatcggcag cgaagcctat ttcgggttga tggtcgagcc
      841 tgacaattcc tcgctgcatc cggcgaaata cgtgcgcggc ctggcgaagg ccgccgagcg
      901 agccggggtc ggcctgcacg agaagacgcc ggtgttatcg ttcgagcgaa atggatcgag
      961 tttcgcggtg aagacgagtg gcggcacgct cgcaacggaa gccttgtttc tcgcgacaaa
     1021 cggttatacc gacgccgccg cccctgcgct gcgccgccgc gtcgtgccga tcggctcgta
     1081 catcatcgcc accgagcctt tgcgcgcaga tgtcgctgcg cgcctgcttc cgaatcggcg
     1141 tgtcgtatcg gactcaaaga attttctgca ctattaccgg ctctctgctg ataaccggtt
     1201 gctgtttggc gggcgagcgg cgttcgtgcc acccactggc gattccaacc gcaagagcgc
     1261 agagattctg cgtcgcgata tgctggaggt ctttcccgag ctggcggatg cgagcgtcga
     1321 gtatgtgtgg agcggcaacg tgtgcttcac gatggacatg ctgccgcatg ccggccgctt
     1381 cgacgacggc gtgcattacg cggcaggcta cggcggccac ggcgtggcga tggcgacgta
     1441 cctcggcgcg aagatggccg acgtcattct cggcaaggac gatcggaatc cattccgcga
     1501 cctgcgcttc agagccattc ccttgtacga cgggcggccg tggttcttac cgctccccgc
     1561 gctgtggttc aagttcctcg actggatccg ataataacct cccgcaggtc gcgtaatctg
     1621 tgggagggtc gcgcggccaa acgcgaaccc accgtcatga aatctcgcct tcctgccaag
     1681 atcggcgcga ggccgccagg gacgcgtaga tgactggata catgcgctcg gcgtaatcca
     1741 gatgcttcag cgactcggcg aaacgcagat ttcccggctc gttgatcccc agcgtttctt
     1801 ccacggtagg cgcgacgatg gtctcgatgc gccccccggt gctggcgata gcctcgatcg
     1861 catcggtcag gtaatactct ttctgggcgt tgttgttgga cagccgatcg aacgccgcga
     1921 gcagcgcgcg acactcgaag cagtacatgc cgacgttgat ttcgcgaagg gccagcgtct
     1981 cgggatcggc atcttgcacc tcgatgatgc gcatcacgcg accgtccgga ccgcgcacga
     2041 cgcgcccgaa atcgggggga ttctccagga tgatggtgag catggaggct ttcacatcgc
     2101 cctggcagcg atcgatgagg cgcttcatag tatccgggtt gatcaggggc atgtcgccgt
     2161 acgcgatgag cacgcggcca gtgaaattgc tcagataggg gcgcgccatc atgacggcat
     2221 gcgccgtgcc gagctgctcc tcctggatgg cgtaacgcac gcttgcgccc agatgcgcca
     2281 tcacctgctc gcgctgatag ccgacgacga cgatgatgtc gcgaatgccg acgcggcgca
     2341 cccgttccac cacgtgctgg atcagactct ggccgtggag cgtgtggagg actttgggca
     2401 ggtcggactt catgcgcgta cctttgcctg ctgccagcac gacggcgatc acggcgtcct
     2461 gcgccgggtc gggaatgtga ggtgtgatgg gacggacttg gcccagcctg gtcatttaca
     2521 tagtctggcc ggtgagatcg gccagcagtc cctgtaactg cgggagccgc tccggcgatg
     2581 cgttgggcga gaatatctcg gtgttgacgc cgaccggaat ctcggtcaga tgcgcgatgg
     2641 gacacagcgc ttccgtgcgc gtccgcacgt cgcggttcaa gacgatgacg cggttcgcgg
     2701 cggccaaccc ggtgtaggcc agcggctggt aacggtcgtc acgattgacg acgaactcga
     2761 tcatgctgcc gtgaacggcg acgacgtacg gcacgctcgt gcggcgcttc acccgcgccg
     2821 caaccgcggg cagcggcacg gcgtggttga cgtgaagcat ttccagccgg tggcgccgta
     2881 ccagttcgac gagctgctcg gtcaactcgt ccaggtagga ttcgagttca tggtcggtaa
     2941 gctgcgtgaa gagcggccgg tcgggcgctt cgacacgggg ataagccacc ggcgccagcc
     3001 cgctcaattg gtggagccag caggccggat cgggctggcg ctgaaaccgc gtgacgacgt
     3061 catgcccgcg tcccgtcgag gcggcgtcga cgaaccaata atcctccggg tgctcttcgt
     3121 agcacaacag cgcgacgtag tgcccgcgtg aggcgagcgt ctcggccaga cgcgccacgt
     3181 agatggcgct gcctgaacct gccaagccat acaggtgacc tatgccgatg cgcatgtcct
     3241 ctgaccctaa cgatgcctgc ggcgcatcat gaacaattcg gtcccggccc gatggctggt
     3301 cacgtagcgt acgtcatcca tgacgtgctg catcaagaat atgccatagc cgccgacggt
     3361 cagctcgccg tccatgttcg gaggccgcac gtgggtcgga tcgaacttgt caccgtagtc
     3421 gcgcagccga aagacgattc gttctccgat gcgtttggca gtgaggatga gatggcattc
     3481 ctgccgatcg gcgtacgcgt agcgtacaac gttggtgacg gcttcgctga cggccagcgt
     3541 gatgtcagcc gtggcgcgct cgtcggcgcc cagcccggcc gctgtctcga cggcccaggc
     3601 gcgaactctg ccgacgattt cgttcccgcc ccggacttcg tatcgatgcg tggctgaaat
     3661 atccacggga tctcgtttct gggtggaaac cgcggcagcc cattcgccgg gtagtgatgg
     3721 agcgcaccag cttactctgc gaaactggcg acggcggctg cgcgctcgtc gaaaatgtcg
     3781 aagacccgat cgaatcgcgt caactccacg acgcgacgca gctctggggg caggttgcac
     3841 agccgcacgt cgccgtgccc gatgcgaatg cgcttgtaga gcctggccag ggcggccaga
     3901 cctgagctgt ccataaaact cacccggccc agatcgatca cgtagttccg cacgccgcgg
     3961 ccgacgagat cgtcgaatac cgcatttagg tcagcgacgg tcgtggtatc cacatcgccg
     4021 cccacggcga caacgacgat ggtgccgaac tcgagggttt cgacctgtat gtgctctctt
     4081 gagacgatgt cgtgaagggt tgtcgagaac atgttagcgc gtccaagtca aacaaaaccg
     4141 ggtcagccat gctgctgtgc cggtatcatc ctagtaggca ctatagaggc ttctaaggcc
     4201 ggctttcgtt ggctgtggga tgtggttgcg tttggattgc gttgtatcag gatgggttca
     4261 tgctgattgc catgcctccg ctttcaagtc ccacgcgctc agcgtctcga gccacgagtg
     4321 ggccgtcagg tcgagctgga taggagtgac ggagatgtag ccgttgtcga tagcccagat
     4381 gtcggtgccc tcatccggca cgccggatgg ccgctcgccg ccgatccagt aatagggtcg
     4441 tccgcgaggg tcctggcgca cgatcagctc atcgcgatac aagcgcttcc ccatgcgggt
     4501 gatgcgcacg ccgtagatgg gcggagccgg gacgttgacg ttgagcagca cgtcgcgcgg
     4561 caggctgcgc tcgaacgcct gcgcggcgag ccgggtggcg aacgcggcgg cggctgggta
     4621 cgcctcggcg gacgccgccg acacggcgat cgaaggcacg ccgccgatcg taccttccat
     4681 ggcagcggtc accgtgccgg agtacgtcac gtcgtggccg aggttacagt tgaggttgat
     4741 gccgctcaac aagaggtcgg gtttgaaatc gagcgcgccg agcagcacga gagccacaca
     4801 gtcgctgggc gcgccggtcg ttgcatacgc tgtcgagccg tcgcgcagat gcacttcgcc
     4861 gatgcgcagc ggcttgtgca tcgtcttgac gtgccctgcc gcgctgtagt tgtgctcggg
     4921 cgcgacgacg acgacgttac ccaccctgga cagcgccgtt ttcagcgcga gcaggccggg
     4981 cgcggcgtat ccgtcgtcgt tggtgacgag aatgttcatc cggagggtct cctcaaagag
     5041 cgatagccga aagtatatca cgagcgcggg aggcggcggc ttcgagcagg gcgcgttgaa
     5101 gcgtctcgtc cgccgcaagc cgtgagcgtg ttataatcgc cccaacgcat agcgcagctt
     5161 ctggccgcaa tccgagagcg atccgcgaat ctacgcgaac agcgcgaaga atgagtggcg
     5221 attcgcgtta ctcgcgcatg aaaaacctcg cggccggcgc acttcgtgaa ttatcattgg
     5281 aggaagccaa acccatggtc agccctgaac ccatcgcgcc cgcggacggt gcgaagatcg
     5341 ctattcagaa cggcaagctg cgcgtgccgg attatccgat cattcctttc attgaaggag
     5401 atggcaccgg ccccgacatc tggcgcgcca gcgtgcgcgt gttcgatgcg gcggtgcaaa
     5461 aagcgtacgg cggcaagcgc cagatcaaat ggatggaagt ctacgccggt gagaagtcgt
     5521 tcacgcagtt cagcacctgg ctgccggaag agacggtcga ggctttccgc gaatacctgg
     5581 tcggcatcaa aggccccctg acgacgccag tcggcggtgg cattcgatcg ctgaacgtcg
     5641 cgctgcgcca gttgctcgat ctgtacgtgt gcctgcggcc ggtgcgctgg ttcaagggcg
     5701 tgccatcgcc ggtccggcgc cctgaaagag
//
LOCUS       DBNHXB010000322         7946 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2603514, whole genome shotgun sequence.
ACCESSION   DBNHXB010000322 DBNHXB010000000
VERSION     DBNHXB010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7946)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7946)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7946
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2603514"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="AC162G_13005"
     CDS             complement(<1..775)
                     /locus_tag="AC162G_13005"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR5076769.1"
                     /translation="MDNPSWGGLDPEQFRLYGKQVIDWIADYLAKAERYPVLSPLNPG
                     DVKRQLPAGPPQTPESMDAILADFDRVILPGITHWNHPGFFAYFAISGSAPGILGELL
                     SAALNVNGMLWRTSPAATELEDVVMDWLRQMIGLPPVFEGVITDTASVSTLIAIAAAR
                     EALDRRVREDGLAGRADLPRLRLYCSEQAHSSVEKAGIVLGLGQAGVRKIPVDDEFRM
                     RSDLLAAAVDEDRRAGWLPFCVVATVGTTSTTSIDPVPAI"
     gene            complement(794..1243)
                     /locus_tag="AC162G_13010"
     CDS             complement(794..1243)
                     /locus_tag="AC162G_13010"
                     /inference="COORDINATES: protein motif:HMM:NF025210.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR5076770.1"
                     /translation="MDVTTLSPDLRRLFGSARLYSDGRLYAVVSLPRDQMRAATILFG
                     GLAEPFAAMIVDRDEITIVMHEMDWSVAGRDLPGMRVLNDYRLITLDVELAADVTGFM
                     DAVCRLLAGAGVPMLPLAAYSRDHILVRARDLDRTWQVLSDCIAACR"
     gene            complement(1253..2110)
                     /locus_tag="AC162G_13015"
     CDS             complement(1253..2110)
                     /locus_tag="AC162G_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076771.1"
                     /translation="MQPLRTDTELPRLAIVPTRSLVPHEEFDLKRSEPLAERLRAEGV
                     LKNPPVVAPVEGEDRYVVLDGTNRLVALSVLACPHTVVQIVDYDDPNLVLDTWSHLIT
                     DMPPDEFTVAMRTVDGLRIEMATHLHARAELARRQAAAYAVLPGARAPDGVYLLYVDG
                     DARQRTATLARLVNLYQARGHIHRVKTDHLEWLLPLHEHVTALVVFPRYEPSEIIELA
                     RQGAALPTGVTRHVIPGRALRLNFPLSVLLDDRSLDEKNAWLQDWLRAKLAGKEVRYY
                     QESTYLFDE"
     gene            complement(2300..3706)
                     /locus_tag="AC162G_13020"
     CDS             complement(2300..3706)
                     /locus_tag="AC162G_13020"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HIR5076772.1"
                     /translation="MYATDVHSNVYSNVLETIGHTPLVRLNRVVSGLRATVLAKLEFL
                     NPGGSVKDRIGAAMIEDAEAAGKLRPGGTIVEATSGNTGVGLAIAAAIKGYRCVFVMP
                     DKMSQEKVQLLRAFGARVVITPTAVEPDDPRSYYSVSRRIAAETPNSILADQYHNPMN
                     PDTHYRTTGPEIWEQTGGRVKVLVAGMGTGGTITGTGRYLKERNSDVKVVGVDPVGSL
                     LYDTWRLGRVPEEPFLKTYKIEGIGEDFLPGALDLSVCDEVVQSDDKESFLITRRLVR
                     EEGIFCGGSSGSAVAGVLKSRLVRDLDRDAVAVVILPDSGSRYLSKVFDDEWMRENGF
                     LEAPWGAATVADLLAAKPSREVISVSPLEKVRNVIARLHEHDISQMPVISDGKLAGMV
                     TETQLLNHLVSGEAAADDPIAPIVQRQVTTVSGETSLETVMGLFGDGSVAVVLDGDEV
                     AGIITKIDLIDFLAARSK"
     gene            3886..5139
                     /locus_tag="AC162G_13025"
     CDS             3886..5139
                     /locus_tag="AC162G_13025"
                     /inference="COORDINATES: protein motif:HMM:NF019311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR5076773.1"
                     /translation="MKTARLLVSLAIVLGVLAGIVPVASAQACATTYVVQRGENLYRI
                     SLKFGVSMAALAQANYIVNPDRIYAGQVLCIPGSGYPPPPPPPPPPGQCIYYTVKPGD
                     SLGSIAIRYGTTVSAIQRANNIRNPNVIYAGQVLCIPTSGGRPPAFPQWRGEYFNNMN
                     LAGAPSVVRNDAAINFNWGQGWPDTRISADHFSVRWTRTLLFNAGTFRLTARADDGIR
                     VFVDNVLIIDEWHPATGQQYTADVTLATGNHTFRVEYFEDSGLASAFFTWARVTGPTL
                     PIPTPGGATPAPGGGGGAWTACFWTNRNRDVSGQPTFCRFDPWIAFNWGTGSPDALIS
                     PDVFSARWTSSQTFQAGTYRFFALIDDGVRLYVDNKLVIDEWFEHNGVTLVGDVTLSA
                     GAHAVTVEYFEFGHTAQIYVWWEKR"
     gene            complement(5278..5664)
                     /locus_tag="AC162G_13030"
     CDS             complement(5278..5664)
                     /locus_tag="AC162G_13030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5076774.1"
                     /translation="MNILVVEDDPDARKVLSLILKLDGFEVSTVTGGHEAIDAIRAAA
                     PDLILLDVMMPGVDGYEVCRRIRSEPATRGIPIVMLSGKADRESVVRGLAAGANEYLS
                     KPIKPSSLTKQLRATLMRTAAKMPAS"
     gene            complement(5694..5930)
                     /locus_tag="AC162G_13035"
     CDS             complement(5694..5930)
                     /locus_tag="AC162G_13035"
                     /inference="COORDINATES: protein motif:HMM:NF024139.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5076775.1"
                     /translation="MSEYATEMRLSPYGDRVDGLLTAREAAAFLRVSLNTLWRMERRN
                     LITPYRTPGGHRRYSVDMLRTYLERTRVRSWELE"
     gene            6108..6905
                     /gene="yqeC"
                     /locus_tag="AC162G_13040"
     CDS             6108..6905
                     /gene="yqeC"
                     /locus_tag="AC162G_13040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HIR5076776.1"
                     /translation="MTDAIGLQEGDALACVGAGGKTTTCWRLWAESRARGWLPIFTTT
                     TRILEPILSDDSALLLSNDPRPARIRQVMCEVSGLILAASRLPDAVNDALPNPIAPAL
                     PSKLAGLQSEQLDRLIEALPEMTWLVEADGARGRGLKLPAAREPVIPARTTLVAVLMH
                     MDVIGHPLDEVAAHRPEALAAYLGLPMGHPISASHISRLLVDPAAGLKAVPAGPRVVA
                     VLNQRDESAPRPETRQVARAVLASGRYERVLSASLQARSPVLEVFTR"
     gene            6902..7498
                     /locus_tag="AC162G_13045"
     CDS             6902..7498
                     /locus_tag="AC162G_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5076777.1"
                     /translation="MTTKRIAGLLLAAGESARLGQPKQLLEWNGTPLIEHAARTALCA
                     GLDPVVVVIGSRAGDMRAALKSPVTIVENPDWQAGMSTSLRAGLRALPGDVDAAIMLL
                     VDQPRLSEAHLRALIDTYERLKKAIVVSAHRGRRASPALFDRSLFDQLMRVSGDLGGR
                     PVIEANPHEVVQVEAGDELQLADVDTWEEWQRMSSRFA"
     gene            7508..>7946
                     /locus_tag="AC162G_13050"
     CDS             7508..>7946
                     /locus_tag="AC162G_13050"
                     /inference="COORDINATES: protein motif:HMM:NF024740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076778.1"
                     /translation="MARRAGFNSAFVMDYSARQYGMTRSSLSSLQALIIDMDGVLWRG
                     SEALPGITDFFAFLRRRAIRFVLATNNASRTEAQYAERLASYGAAVAIEEIVTSSTAT
                     AEHLTSLVPRGSRVYAIGMDGVREALAKQGFVLSEGDEAAAVVV"
BASE COUNT         1430 a         2588 c         2492 g         1436 t
ORIGIN      
        1 cgatagccgg aaccgggtcg atgctcgtcg tcgaggtcgt gccgacggta gccacgacgc
       61 agaacggcag ccagcccgcg cgccgatcct cgtcgacagc tgcggcgagc agatcggacc
      121 gcatgcggaa ctcgtcgtcg accgggatct tgcgcacacc ggcctgccct aatcccagca
      181 cgatcccggc cttctccacc gacgagtgcg cctgctcgga gcagtacagc ctcagtcgag
      241 gaaggtctgc ccggccggcc agcccatcct cgcgcacgcg ccggtccaac gcctcgcgcg
      301 cggcggcgat ggcgatcagg gtagagacgg acgccgtatc ggtaatcacc ccttcaaaca
      361 cgggcggcaa gccgatcatc tggcgcagcc agtccatcac gacgtcttcc agctcggtcg
      421 ccgccggcga ggtccgccac agcatgccgt tgacgttgag cgccgcgctg agcagctcgc
      481 ccaggatacc cggcgcagat ccgctaatcg caaagtaggc gaagaacccc ggatggttcc
      541 aatgcgtgat gccgggtagg atcacccggt cgaaatcggc gaggatcgcg tccatcgact
      601 cgggcgtctg aggcggcccg gccggcaact gtcgcttgac gtcgccggga ttgagaggag
      661 agagcaccgg ataacgttcg gctttcgcca ggtaatcggc gatccagtcg atcacctgct
      721 tgccgtagag gcggaactgc tcagggtcaa ggccgcccca agatggatta tccatgttca
      781 cttccttgtt tgtttacctg cacgccgcaa tgcagtcaga cagaacctgc catgtccggt
      841 cgagatcgcg cgcacggaca aggatatgat cgcgggaata cgccgcgagc ggcagcatcg
      901 gcacgccggc cccggccagc aggcggcaca cggcgtccat gaagcccgtc acgtccgccg
      961 ccagttcgac gtcgagcgta atcaggcggt agtcgttcag gacgcgcatt cccggcaaat
     1021 cgcggccggc cacggaccag tccatctcgt gcatgacaat cgtgatctcg tctcggtcga
     1081 cgatcatagc tgcaaacggc tcggcaagcc caccgaagag gatcgtcgcc gcgcgcatct
     1141 ggtcgcgcgg gaggctcacg acggcgtaga gcctgccgtc cgagtataac cgcgcggaac
     1201 cgaacaggcg gcggagatcg ggtgaaagag ttgtgacatc catggcgtag cctcactcgt
     1261 cgaacaggta agtcgattcc tggtaataac gtacttcttt gccggccagc ttcgcacgga
     1321 gccaatcctg cagccaggcg ttcttttcgt cgagcgaacg atcgtcgagc aggacactga
     1381 gcggaaagtt cagccgtagc gcgcggcccg gaatcacgtg gcgtgtgacg cccgtcggca
     1441 gagcggcccc ctgccgcgcc agctcgatga tctcggacgg ctcgtagcgc ggaaagacga
     1501 cgagcgccgt gacgtgttca tgcaggggca gcagccactc caggtgatcg gttttgacac
     1561 gatgaatgtg cccgcgcgcc tgatacaggt tgacgagccg cgcgagtgtc gcggtgcgct
     1621 ggcgcgcgtc gccgtcgacg tagagcagat aaacgccgtc cggcgcgcgc gcgccgggga
     1681 gcacggcata cgcggccgcc tgacgccgcg ccagctcggc gcgggcatgc aggtgcgtcg
     1741 ccatctcgat gcgtaggccg tcgaccgtgc gcatggcaac ggtgaactcg tcaggcggca
     1801 tgtccgtgat caggtgtgac caggtatcga gcacgaggtt cggatcgtcg taatcgacga
     1861 tctgcacgac ggtatgaggg cacgccagca cgctcaaggc gaccagacgg ttggtgccgt
     1921 cgagaacgac gtagcgatcc tcgccttcga ccggcgcgac gaccggcggg ttcttcagca
     1981 caccctcggc gcgcaggcgc tcggcgagcg gctcgctccg cttcagatcg aactcttcat
     2041 gcggcacgag cgaccgcgtg ggcacgatcg ccaggcgggg aagctccgta tcggtgcgca
     2101 agggttgcat cgtcggagat cgctcctctc ggactgcgtt gggtgaactt caaactccaa
     2161 aggttcaaac gacaaaaggt caaacggccg gctcacttgt gtgcacgcaa atgtgtcagg
     2221 aaagcaggcc gggctcccac gcccggctta tccggcggcg tgggagccgc ctccgctgac
     2281 atgttttgcg tgcacacgct cacttcgagc gcgcggcgag gaagtcgatc aagtcgatct
     2341 tcgtgatgat tccggcgact tcgtccccat ccaggacgac cgcaaccgag ccgtcgccaa
     2401 acaggcccat caccgtctcc aaactggtct cgccgctgac cgtagtgacc tgacgctgga
     2461 cgatcggcgc aatggggtca tccgccgctg cttcgccgga cacgagatga ttgagcaatt
     2521 gggtctccgt caccattccg gcgagctttc catcgctgat gacgggcatc tgcgaaatgt
     2581 cgtgctcgtg caggcgtgca atgacgttgc gcactttttc gagcggcgat acagaaatca
     2641 cttcgcggga cggctttgcg gccagcagat cggccaccgt cgctgcaccc cacggcgctt
     2701 ccaggaagcc gttctcgcgc atccactcgt cgtcgaagac cttgctgaga tagcgcgagc
     2761 cggagtcagg caggataact accgcgactg cgtcgcgatc cagatcgcgc acgagcctgg
     2821 acttgagcac gccggccact gccgagcctg acgatcctcc gcagaagatg ccttcctcgc
     2881 gcaccaggcg gcgcgtgatt aagaacgact ctttgtcgtc ggactgcacg acctcatcgc
     2941 acacgctcag atcgagcgcg ccaggcaaga aatcttcgcc gatgccctcg atcttgtacg
     3001 tcttgagaaa cggctcctcc ggcacgcgcc ccagccgcca cgtgtcgtag agcagcgaac
     3061 ccaccggatc gacgccgacg accttcacgt cggagtttct ctccttgagg taccgacccg
     3121 tgccggtgat cgtcccgccc gtgcccatcc cggctacgag caccttcaca cggcctcccg
     3181 tttgctccca gatttccggg ccggtcgtac gatagtgcgt gtccgggttc atcggattgt
     3241 gatactgatc ggcaaggatg ctgttgggcg tctcggcggc gatgcggcgg ctgacactgt
     3301 agtaggagcg tggatcgtcc ggctcgacgg cggtcggcgt gatgacgacg cgcgcgccga
     3361 aggcgcgcag gagctgcact ttctcctggc tcattttgtc gggcatgaca aacacgcagc
     3421 gatacccttt gatcgcggcg gcgatcgcca gccccacgcc ggtattgccc gacgtcgcct
     3481 cgacgatggt gccgccgggc ctcagcttac ccgccgcttc ggcgtcttcg atcatcgccg
     3541 cgccgatgcg atccttcacc gagccgcccg ggttgagaaa ctccagcttg gcgagcacgg
     3601 tcgcgcgcag gccgctcaca actcgattca aacgaacgag cggcgtatgg ccgatcgttt
     3661 ccagcacgtt agaatatacg ttcgagtgca catctgtcgc atacatgtga cccctccaga
     3721 agccagccgc gccctgacca atcccccctc ggcgcgctag agagattagc acgaaagaaa
     3781 ggcggtgtca aaggatttgc gcattgtgcc cttacgttgt atgattcccg gcgctcggag
     3841 ccgcgctgca catcgttggg tgggccacca tctaaggaga ctcttatgaa aaccgcgcga
     3901 ttgcttgtgt cgctcgcaat tgtcctgggt gttctggctg gtatcgtccc cgtcgcttct
     3961 gcgcaagcgt gcgccacgac atacgttgtg cagcggggcg agaacctgta ccgcatcagc
     4021 ctgaaattcg gcgtgtccat ggccgcgctt gcgcaggcca attacatcgt caacccagac
     4081 agaatctacg caggacaggt gctatgtatc ccaggcagcg gctatccgcc tcctcccccg
     4141 ccgcccccgc cccccggcca gtgcatctac tacaccgtca agccaggcga ctcgctggga
     4201 tcgattgcca tccggtacgg cacgacggtg tcggccatcc agcgggcaaa taacatcagg
     4261 aacccgaacg tgatctacgc cgggcaagtg ttgtgcattc ccacttctgg cggcagaccg
     4321 cccgctttcc cacagtggag aggcgaatat ttcaacaaca tgaacctggc cggcgcgccc
     4381 tctgtcgtgc gtaacgacgc ggccatcaac ttcaactggg gccagggctg gcccgacacg
     4441 cgcatcagcg ccgaccactt ctcggttcgc tggacgcgca cgcttctctt caacgcaggc
     4501 acattccggc tgacggcccg cgccgacgat ggcattcgcg tattcgtcga caacgtgctg
     4561 atcatcgacg agtggcatcc ggcgaccgga cagcaataca ctgccgacgt gacccttgcg
     4621 accggcaatc acacatttcg agtggaatac ttcgaagaca gcggcttagc ctctgccttc
     4681 ttcacctggg cgcgcgtcac cggccctacc ctcccgatac ccacgcccgg cggagcgacc
     4741 ccggcaccgg gcggtggtgg cggcgcgtgg acggcgtgct tctggaccaa tcgaaaccgc
     4801 gatgtcagcg ggcagccgac gttttgccgg ttcgatccgt ggatcgcgtt caactgggga
     4861 acaggttcgc ccgatgccct gatctcaccc gacgtcttct cggcgcgctg gacgagcagc
     4921 cagacgttcc aggccggaac ttaccgcttc ttcgctctga tcgacgacgg ggtgcgactg
     4981 tacgtggaca acaagctcgt catcgacgaa tggtttgagc acaacggcgt cacgttggtc
     5041 ggcgatgtga cgctcagcgc cggggcccat gcggtcacgg tggaatactt cgagttcggg
     5101 catacagcgc aaatctacgt ctggtgggag aagaggtagg catgaccggc gatgtgccgg
     5161 gtgcgcctgc aaaaatcgtg gaccgtaacg ggggactccc cgacctgagg cggtcgggga
     5221 gtttcttttg gcgctcgcgg cgggcgggca gcgcggcgct tcttaatggt gcgtccgcta
     5281 cgacgccggc atcttggccg cggtccgcat gagcgtggcg cgtagttgtt tggtcaggct
     5341 gctgggcttg atgggtttgg acaggtactc gttcgcgccg gccgccaatc cgcgcacaac
     5401 gctctcccga tcggctttcc cggacagcat cacaatcggt atgcccctcg tggccggctc
     5461 cgaccggatg cggcggcaca cctcgtagcc gtccacgccc ggcatcatca catccaacag
     5521 gatcaggtca ggagcggcag cgcgtatcgc gtcgatcgcc tcgtgtccgc ccgtgacggt
     5581 ggatacctca aaaccgtcga gcttcagaat caggctcaac actttccggg catccggatc
     5641 gtcctcaacg acgagtatat tcattcgtgc actcctgcgc caatcactcc agattactcc
     5701 aactcccagc tacgcacgcg cgtgcgctcg agatatgtgc ggagcatgtc caccgaatag
     5761 cgtcggtggc cgccgggcgt gcggtacggg gtgatcaggt tgcgcctctc catgcgccac
     5821 agcgtgttca agctcacacg caggaatgcg gcggcctccc ttgccgtgag cagcccgtcc
     5881 acccgatcgc cgtatggact caatcgcatt tcggttgcat attcgctcat tcgttcacct
     5941 ctcatgctcc cgcccctcaa atgtggccga aaaactgtgg gcggtcatgt cgcctacagt
     6001 atcgcaggtg aggtcatttg ggtaagtttg atgtcaattt gcccagcgtt gggtttgggt
     6061 tggcggagcc gatgcaatag gattctggta caattcgatt tgtgctgttg acggatgcga
     6121 ttgggctgca agagggagac gcgctcgcgt gtgtcggcgc gggcggaaag acgacgacgt
     6181 gctggcgtct atgggcggaa tcccgggcaa gaggatggct gcccatattc acgacgacga
     6241 cgcgcatact cgagccgata ctctccgacg attcggcgct actcctctcg aacgatcccc
     6301 gcccggcgcg cattcgacag gtcatgtgtg aggtcagtgg gctgatcctc gccgcgtcac
     6361 gcctccctga cgcggtgaac gatgccctgc ccaatcccat cgcacccgcg ctgccgagca
     6421 aactcgccgg gcttcaatcg gagcagctcg atcggctcat cgaagcgctg ccagaaatga
     6481 cgtggcttgt agaagccgac ggggcgcgtg gacgtggcct gaagctcccg gcggcacgcg
     6541 agcctgtcat tccggcccga acgaccctcg tcgcggtgtt gatgcacatg gacgtgatag
     6601 gccatccgct cgacgaggtc gccgcgcacc ggcccgaggc gttggcagcg tatctcggct
     6661 taccgatggg tcatccgatc tccgccagcc acatctcacg cttgctcgtc gatccggctg
     6721 ccggactcaa ggccgttcct gccgggccgc gcgtggtggc cgtgctgaat caacgtgacg
     6781 aatccgcgcc gcgcccggaa acgcgccaag tcgcccgggc agtgctggcg tccggccgat
     6841 acgagcgcgt gctcagcgca tcgctgcaag cgcgtagccc tgtcctcgag gtcttcacgc
     6901 gatgacgacg aagcgcatcg ccgggctgct cctggcggcc ggggagtcgg cgcggctggg
     6961 gcagccgaaa caattactcg aatggaacgg cacgccgctc atcgagcatg cggcgcggac
     7021 ggcgctatgt gccgggctgg atccggtggt cgtcgtgatc ggtagccgcg cgggggatat
     7081 gcgcgctgcg ctcaagtcac cggtgacgat cgtcgagaat ccggactggc aggcaggcat
     7141 gagcacttca ctgcgggccg gactgcgggc gctgcccggc gacgtcgacg ccgcgatcat
     7201 gctgctcgtc gatcagccgc gcctgtccga ggcgcacctg cgcgccctga tcgacaccta
     7261 cgagagactg aagaaggcca tcgtcgtgtc ggcgcaccgg ggccgccgcg catcgccggc
     7321 gctgttcgac cgctcgctgt tcgatcagct catgcgtgtc tcaggcgacc tcggcggccg
     7381 gccggtcatc gaagcgaacc cgcacgaggt cgttcaggtc gaagccggag acgagctgca
     7441 gctcgcggat gtcgatacat gggaggaatg gcaacggatg agcagccgct tcgcgtaaac
     7501 ggatgcgttg gcacggcgtg ccggcttcaa ttccgctttc gtcatggact actccgcccg
     7561 ccaatatggc atgacccgat cttcattgtc gtcacttcag gcgctcatca tcgatatgga
     7621 tggcgtgttg tggcgcggca gcgaagcgct ccccggcatc actgatttct tcgcgtttct
     7681 gcgccgccgg gccattcgct tcgtactggc caccaataac gcctcgcgca ccgaggcaca
     7741 gtacgccgag cggttggcct cttacggcgc ggcggtcgcc atcgaagaga tcgtcacgtc
     7801 ctcgactgcg acggccgagc acctcacgtc gctcgtgccg agaggatcgc gcgtgtacgc
     7861 catcggcatg gacggcgtgc gcgaggccct ggcaaaacag ggcttcgtcc tgagcgaagg
     7921 cgacgaggct gcggctgtcg tcgtcg
//
LOCUS       DBNHXB010000323         5849 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1764961, whole genome shotgun sequence.
ACCESSION   DBNHXB010000323 DBNHXB010000000
VERSION     DBNHXB010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1764961"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..553)
                     /gene="mscL"
                     /locus_tag="AC162G_13055"
     CDS             complement(176..553)
                     /gene="mscL"
                     /locus_tag="AC162G_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR5076779.1"
                     /translation="MLKEFRDFIARGNVIDLAVAVIIGVAFGAIISSLVNDILMPLIG
                     LVLGGIDFTSLSIQVGDAVVKYGNFIQAVVNFLIIAFVLFLIVRSYNKTQKKEEKAPA
                     PPPEPPAEVRLLGEIRDLLRERK"
     gene            complement(613..3678)
                     /locus_tag="AC162G_13060"
     CDS             complement(613..3678)
                     /locus_tag="AC162G_13060"
                     /inference="COORDINATES: protein motif:HMM:NF013125.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HIR5076780.1"
                     /translation="MKIARVSIVIVVGCVAVLGIIGLLRPVEAAPVQTGKLSASPRDV
                     VFNEVAWGGTSADANDEWIEFKNDTASPIDLTGWSLRSGDGSPNIVLSGAIPPNGYFL
                     LERTDDTTVSDMPADQIYTGGLANTGETLTLADTLSDVVDTTNANGGAWPAGSGSPTF
                     FSMERVDPSAADADANWASNDGVTRNGLDANGNPINGTPKQPNSRAGALPPPSPPGSV
                     LISAVHFDAYANGDEGFRLTSVHTQAITLTNWFATDGEGTLNLSGTLEAGQSIWIAKR
                     AVTFTQQFGFKPNYEYEGNTDSLVPDLTGSAPLLGDNDELAIRQGASNWIDAVVWGAG
                     QVTDTGWLTGWMGPGVQRYSNSSIGAFGQILYRKLDEATNAIVADTHTAVDWANDSAD
                     PISGRKTMYPGWDLESFWEPARITASARLTVAIAPDNAYRVISDLLGSARQSIKIEMH
                     TFEHPALAGVLTRTIQDYGVSVTVLLEGGPVGGIEDQELWICQQIEAAGGDRWFMIND
                     SSADIRDRYDYLHAKMIVVDDRVAAIGSENLSLNSLPTDDFSDGTIGRRGVYLVTDAP
                     GVVTRALQIWAADFDPANHRDIHPWSAGDPKYGAPPLGFTPVYTSGGSGYAIRYPSPL
                     SLTAPLTFEVLTAPESSLRASDALLGLIAQAAPGDSIDAEQLEERPHWGSSSSNPIDD
                     PNLRLQALIGAASRGARVHLLLDGHFDDPLSPVSNAATYAYVESLRAISPVLASHLEV
                     RLGNPTLSGVHNKMFLFNVGGRKIVHAGSLNGSEASHKVNREIALQVESEVAYAYLYA
                     MFEHDWAFRPRVYLPLILRNYMPPADHLLVSKVFYLGTPGVDEWMQIYNPTAITVSLS
                     GFKIGDEETRGGGGFGFDGMWEFPPAATIAPGQKINIAGTFAGFYARFGYDPNFAFFD
                     GVAGVNRMTPYLAWTSAATLTLANAGDEVLLLGPGDQLIDGVAWGTGGLPGHASCPAI
                     APPPYASIERTPIWQDTDNCPADFVTNPSPLPATAADRLSLIAYRRFRS"
     gene            4248..4862
                     /locus_tag="AC162G_13065"
     CDS             4248..4862
                     /locus_tag="AC162G_13065"
                     /inference="COORDINATES: protein motif:HMM:NF018213.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HIR5076781.1"
                     /translation="MWRKTECSLKANTVEKKSLTDLSILWRRLDQPGHESARLFFQHP
                     DWHLTGTAVFAHHQQPCRLDYLVVCDSGWQTLSGKVAGWVGSETVELELSVDSARRWR
                     LNGTERPEVAGCIDLDLNFSPSTNLLPIRRLGLAIGQEANVAAAWLRFPSFALEPLEQ
                     LYRRTDAATYRYESAGGQFVTELQVNTAGFVTYYPNFWQIEEVV"
     gene            4903..>5849
                     /locus_tag="AC162G_13070"
     CDS             4903..>5849
                     /locus_tag="AC162G_13070"
                     /inference="COORDINATES: protein motif:HMM:NF015038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HIR5076782.1"
                     /translation="MTTLPTLDQLNELSRDELLALIVQLLAEIETLRAEIERLKQPPT
                     SSRNSSQPPSRDWKGNRPAGKRRQPHGAKAGHVKAERPLVDNPTTVIEAPVVVCTACG
                     ADLREVLPARTIRRQVTELPEIQPVVIETRQHEVVCPCCQALQRGVLPEGLEASRQFG
                     PRLEAVVAYFHQAQHMGFERTQTVLRDLFGTTLSEGGAVAILERAGAAAQPEAEAIGE
                     QVRQSAVIGSDETSARVNGRNWWEWVFESPVGEYHLIVPSRGQDVIDTFMGERRAEVW
                     RSDCWKPQLNAPADQHQLCLSHQIRALQGVIDRRPRLRWA"
BASE COUNT         1064 a         1765 c         1886 g         1134 t
ORIGIN      
        1 ccacggcgac gcggcggcca acctcgacgc tgcccccgac gcgcacgccg tcacgcacgc
       61 cgacggcgac gcccaggtaa ccgaaaacac aacgtgcgac acacttccgg acccccttcg
      121 cttcgctgcg agagcgagag tgcgccgcac gtatgatgca cctgggagaa gagtgttact
      181 tgcgctccct gagcaagtcg cggatctccc ccagcagcct cacctcggcc ggaggctcgg
      241 gcgggggcgc gggcgctttc tcttccttct tttgcgtttt gttgtaactg cgcacgatca
      301 ggaacagcac gaatgcgatg atcaggaagt tgacgacggc ctggatgaag ttgccgtact
      361 tgaccacggc atcacccacc tggatggaga ggctggtgaa atcgatcccg cccagcacca
      421 ggccgatcag tggcatgagg atgtcgttga cgagagaact gatgatcgcg ccaaatgcga
      481 cgccgataat cacggccacg gcgaggtcga tcacgttgcc gcgcgcgatg aagtcacgga
      541 actcttttag catggttcgc tccttctgcc ggcgtgggga tgtccaaaat tatacttgta
      601 gatcgacgca ggtcaagatc gaaatcggcg atacgcgata agcgataagc gatccgctgc
      661 cgtagccggc agcggcgacg ggttcgtcac gaagtctgcg gggcagttat ccgtatcttg
      721 ccagataggc gttcgctcga tggatgcgta cggcggcggc gcgatcgccg ggcaggatgc
      781 gtgacccggc agccctccag ttccccacgc cacgccgtcg atcaattgat cgcccggtcc
      841 cagcagcagc acctcgtcgc ctgcgttggc cagggtgagc gtcgccgcgc tcgtccaggc
      901 gagatacggc gtcatgcggt tgacgcccgc cacgccgtcg aagaaagcga agtttgggtc
      961 gtaaccgaat cgagcgtaga aaccggcgaa cgtcccggcg atgttgatct tctgcccggg
     1021 cgcgatcgtc gccgccggcg ggaactccca catcccgtcg aagccgaatc cgccgccgcc
     1081 tcgcgtctct tcgtcgccga tcttgaagcc gctcaacgac acggtgatcg cagtcggatt
     1141 gtagatttgc atccactcgt cgacgccggg cgtgccgagg tagaagactt tgctcaccag
     1201 gagatggtct gcgggcggca tatagttccg caagatgagc ggcaggtaca cgcgcggccg
     1261 aaatgcccag tcgtgctcga acatggcgta caggtacgcg tacgcgactt ccgactccac
     1321 ctgcagtgcg atctcgcggt tgaccttgtg ggatgcctcg ctgccgttca ggctgccggc
     1381 gtgaacgatc ttgcgtccac cgacgttgaa tagaaacatc ttgttgtgga cgccgctgag
     1441 cgtcgggttg ccgagccgca cttcgagatg gctcgcgagt acggggctga tcgcgcgcag
     1501 cgactcgacg taggcgtatg tcgccgcgtt gctcacaggc gacaacggat cgtcgaagtg
     1561 cccgtcgaga agcagatgga cccgcgcgcc gcgtgacgcc gcgccgatca gcgcctgcag
     1621 gcggagattg ggatcgtcga tcgggttgct cgacgagctt ccccagtgtg gacgctcctc
     1681 caactgctcg gcgtcgatgc tgtcacccgg ggcggcctgc gcgatcaagc cgagcagggc
     1741 gtcgctcgcg cgcaggctgc tttcaggcgc ggtcagcacc tcgaaggtga gcggcgccgt
     1801 caggctcaac ggcgacggat agcggatggc gtagccgctc ccgccgctgg tataaaccgg
     1861 cgtgaagccg agaggcggcg cgccgtactt cggatcgccg gccgaccacg ggtggatgtc
     1921 gcgatgattg gctgggtcaa aatcggcggc ccagatttgc agcgcgcgcg tcacgacgcc
     1981 gggcgcgtcg gtgaccagat acacgccgcg ccggccgatc gtaccgtccg agaaatcgtc
     2041 ggtgggcagg ctgttgaggc tgaggttctc gctgccgatc gccgcgacgc ggtcatcgac
     2101 gacgatcatc ttggcatgca ggtaatcgta ccggtcgcgg atgtccgcgc tgctgtcgtt
     2161 gatcatgaac cagcggtcgc cgcccgccgc ttcgatctgc tggcagatcc acaactcctg
     2221 atcctcgatg ccgcccaccg gcccgccttc gagcagaacg gtcacgctca cgccgtagtc
     2281 ctggatcgtg cgcgtcagga cgccggcgag cgcgggatgc tcgaacgtgt gcatttcgat
     2341 cttgattgac tgccgcgccg agccgagcag atcggaaatg acgcggtaag cgttgtccgg
     2401 cgcgatggcg acggtgaggc gcgcgctcgc cgtgatccgg gcaggctccc agaacgattc
     2461 gagatcccag cccggataca tcgtcttgcg gcccgagatg gggtccgcgc tgtcgtttgc
     2521 ccaatcaact gccgtatggg tgtcggcgac gatggcgttc gtcgcttcgt cgagcttacg
     2581 gtagaggatt tgcccgaagg cgccgatact gctgttgctg taccgttgaa cgcccggtcc
     2641 catccagccg gtcagccatc ccgtgtcggt gacctggccc gcgccccaca cgacggcgtc
     2701 gatccagttg ctcgcgccct ggcggatggc aagctcgtcg ttgtcgccga gcagcggcgc
     2761 gctgccggtg agatcgggga ccagtgaatc ggtgttgccc tcgtactcgt agttgggctt
     2821 gaagccgaat tgctgcgtga aggtgacggc gcgcttggcg atccagatcg actggccggc
     2881 ttcgagcgtg ccgctgagat tgagcgtgcc ttcgccgtcg gtggcgaacc aattcgtgag
     2941 ggtgatcgct tgcgtgtgga cactggtcag ccggaagcct tcgtcgccgt tggcataggc
     3001 gtcgaagtgg acggcgctga tcagcacgct gcccggcggc gaaggcgggg gcagcgcgcc
     3061 cgcccgggag ttgggctgct tcggcgtccc gttgatcggg ttcccgttcg cgtcaagccc
     3121 gttacgcgtg acgccgtcat tgctcgccca gttcgcgtcg gcgtcggcgg cgctcggatc
     3181 gacgcgctcc atcgagaaga aagtgggtga acccgatccg gccggccagg cgccgccatt
     3241 cgcattagtc gtgtcgacga cgtcgctcag cgtgtcggcg agcgtgagtg tctcacccgt
     3301 attggcaagg ccgcctgtat aaatctgatc ggcaggcatg tcgctaaccg tcgtgtcgtc
     3361 tgtgcgttcg agcaggaagt atccgttcgg cgggatcgcg ccggagagga cgatgttagg
     3421 gctgccgtcg ccggaacgca gcgaccagcc cgtcagatcg atcgggctgg ccgtatcgtt
     3481 cttgaactcg atccactcgt cgttcgcgtc ggcggacgtg ccgccccagg cgacttcgtt
     3541 gaagacgacg tcgcgcggac tggcactgag tttccccgtc tgcacgggcg cggcctcgac
     3601 cgggcgcagc aggccgatga tgcccaatac ggcgacgcat cccacgacga tgacgatcga
     3661 tacgcgcgcg atcttcacga cacacctccg ctggctgtcg ccgttaattc ttcaacctca
     3721 aaagcattgt cgccgtcacc ggcgagaggt gtcgattatc ttggtgtctt cgtggtaaag
     3781 attaatagta tagatgcgtt cgcctgattg tgcaaagacc tactcaatgg aagttctttc
     3841 cgcgcttgac ttgccggcgg agctggcata gactggggtt gcgacaatgg ctgcagggga
     3901 ttccgcaaca tgctcttgtc gggggtgtca tacgaagatg atttcatact ctctggcata
     3961 cattctgccg ctcgctctcg ttactattgg tctggtctac atctttggtc gagatctcct
     4021 gtggaagggt caagaattga gcaatcgcct gagaggtgtt gcttcagagc gaactaaaac
     4081 atgggacaca gctagactga tcttcggtat atgtctcgtg gtcgcgggag tcaccgtact
     4141 tcttctctta ctcacataat cacaatggga tcaccgaaat agtcggtcat tccgggcatc
     4201 atcggcaaag tcaatctcaa ccatgacttt cttttaagcg cagtggtgtg tggcgcaaga
     4261 ccgagtgctc actgaaagcc aataccgttg agaaaaagag cttgacagac ctatctatac
     4321 tctggcgcag actggaccag ccgggccatg agtcggctcg actgttcttc cagcatcctg
     4381 actggcatct aaccggaacg gcggtatttg cgcaccatca gcagccgtgc cggctggact
     4441 acctggtcgt gtgcgattcg gggtggcaaa ccttatccgg gaaggtggca ggctgggtcg
     4501 ggagcgagac agtcgagctc gaactctccg ttgactccgc tcgccgctgg cggctcaatg
     4561 gaacagagcg tcccgaagtg gccggctgca tcgaccttga ccttaacttc agtccatcga
     4621 ccaacctgct tccgattcgc cgcctgggtt tggccatcgg gcaggaggcg aacgtggcgg
     4681 ctgcctggct tcgcttccca agcttcgctc tcgagccgct cgaacagctc taccgccgca
     4741 ctgatgccgc gacctatcgg tacgagagtg cgggcggcca attcgtcacc gaactccagg
     4801 tgaacacggc aggtttcgtc acctattatc caaacttctg gcaaatagaa gaggttgtgt
     4861 aactgctcac gacttttttg atagttcggt gttacatata gcgtgacgac cctgcctaca
     4921 cttgaccaac tcaacgaact ctcccgcgac gaactgctgg cgctgatcgt gcaactgctg
     4981 gccgagattg agacgttgcg ggcagaaatc gaacggctca agcaaccgcc gacctcttcg
     5041 cgcaactcgt cgcagccgcc gtcgcgggat tggaaaggga atcggccggc tgggaaacgc
     5101 cgtcagccgc acggggcgaa agcggggcat gtcaaagcgg aacgcccgct ggtggacaat
     5161 ccgacgacgg tgatcgaggc tcccgtggtg gtctgcacag cgtgtggcgc cgatctgcgc
     5221 gaggttctcc cggcgcggac catccgacgc caagtgaccg agttgccgga gatccagccc
     5281 gtcgtcatcg agacccgtca acacgaagtg gtgtgtccgt gttgccaggc gctccagcgg
     5341 ggagtcttgc cggaaggttt ggaagccagc cggcagtttg gcccgcgact ggaagcggtg
     5401 gtggcctatt tccatcaggc acagcatatg ggtttcgagc gcacccagac cgtgttgcgc
     5461 gacctgttcg ggacgacgct gagcgagggc ggcgcagtgg cgattctgga acgggcgggc
     5521 gcagcggcgc aaccggaagc cgaagcgatt ggggagcagg tgcgacagag tgcggtgatt
     5581 ggcagtgatg agaccagcgc ccgtgtcaat gggcgcaact ggtgggaatg ggtgtttgag
     5641 agtcccgtcg gcgagtatca cttgattgtc cccagtcgcg gccaagatgt gatcgacacc
     5701 ttcatgggcg agcgacgcgc cgaagtctgg cgcagtgatt gctggaaacc gcaactgaac
     5761 gcgcccgccg accagcatca actctgtctg agccatcaga tccgcgcttt gcagggggtc
     5821 atcgaccggc gtccgcgctt gcggtgggc
//
LOCUS       DBNHXB010000324        10205 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1933307, whole genome shotgun sequence.
ACCESSION   DBNHXB010000324 DBNHXB010000000
VERSION     DBNHXB010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10205)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1933307"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="AC162G_13075"
     CDS             complement(<1..342)
                     /locus_tag="AC162G_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076783.1"
                     /translation="MSDKSTSPVKTSEVFERLPKSQGERIGVYICHCGSNIAGMVDVA
                     EVRDWAEKRLKHRGVVVSRDYKFMCSSPGQELIEKDIKDHDLTRVVVAACSPHLHEGT
                     FRNACRRAGLNP"
     gene            complement(342..1349)
                     /locus_tag="AC162G_13080"
     CDS             complement(342..1349)
                     /locus_tag="AC162G_13080"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HIR5076784.1"
                     /translation="MNDHYLFYPGCSMESSARAYYDSLMAISEPLGLNLEEIRDWNCC
                     GATEYLGISLTPAYALIGRNLALAAKQADGTRTVVAPCSACYLNLAKADHYMLESPVL
                     SEKVNVALAAGGLHYDPGSLEIRHLLDVIINDIGLDEVKRKVVRPLTGLRVAPYLGCM
                     VPRPDYKKRWSDHEYPNELDRLLKALGAEVIDFPLKTHCCGGHMTQIGPETAFELIRR
                     LISAADQYEADLMATVCPMCQLNIDAYQNETNRHFHTNYRMPIVFFTQLMGLAFGKDP
                     GELGFGRELVSVGEVLNHRIGVEMPEPEAAAPPRKPSRKDKSLPMPQPLHGTSGDEEV
                     S"
     gene            complement(1346..1945)
                     /locus_tag="AC162G_13085"
     CDS             complement(1346..1945)
                     /locus_tag="AC162G_13085"
                     /inference="COORDINATES: protein motif:HMM:NF024581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5076785.1"
                     /translation="MTPHAEPSLDPTNPNLRFLEEVSAATAGVSRLEMCIQCGTCGGS
                     CPPAADMDHTPRMLFAMIRAGMREAALRSNTPWICVSCYHCVVRCPQDVHIADVMYTL
                     KSMAIRAGLYKDKAATETAADFSQTFVDMVEKYGRSFEFGLATRHYLKHFPLRLPGMA
                     PMGFGMLSKKRMDFTPRRIEGIDQLKAILARAKEVEAAA"
     gene            complement(2261..4144)
                     /locus_tag="AC162G_13090"
     CDS             complement(2261..4144)
                     /locus_tag="AC162G_13090"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5076786.1"
                     /translation="MSDSLCTHPAHQGLEDRMRQLEARLEQCTRQLAALNEIGRTLAT
                     TLDLREIYWVMFREIAQGLLATPHLAVALFDQHTETIYYGFAIVDGEEIDPTQLPRIP
                     PGKGPVSDTIRTRQARIVDLREIGQGLTADGRATPVGEERHLLSALYVPMISGDTVIG
                     VMNVQHSEADAFRGIDMTLVSAFASQAAIAIEHAQLFNAEREQWALAEALRDTAAALT
                     STLNLDEVLDRILANVGRVVPHDSADIMLVAGGLAHIARWRGNTGSQPEPKLQAARLA
                     VAEVPYLRQMMRTGQSLAISDTQGHPQWMDIVENLHARSYAGTPIRLKGLVIGFLNLY
                     SATPGFFTASRAERLQVFADQAAVAVENANLYGELERHADELEQRVAERTRELAEANE
                     RLKEVDRLKDQFVSNVSHELRTPLTNTKLYLSLLEHGRPDKRGAYLQTLHRETGRLHN
                     LIEDLLELSRLDLGAAKIHLEPTHINYLAAELIGDRSAMASDRGQLLDCHLDPDLPLA
                     VGDPKRIVQVMSNLMANAINYTPRGGSIVLSTAIRPWGDRDWITFTVRDTGRGIPAEE
                     LPNLFKRFFRGEAGLASGAPGTGLGLAICKEIVDRMEGRISVESQPGQGTAFTVWLRP
                     AHR"
     gene            4507..6975
                     /locus_tag="AC162G_13095"
     CDS             4507..6975
                     /locus_tag="AC162G_13095"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HelD family protein"
                     /protein_id="HIR5076787.1"
                     /translation="MNAQDHPEYAQEHAHLQRALETLASRLEALAQADLMTAYDNAAE
                     ALLMQYRAEYRALKSVSDSPYFARVDFAPHDPSTTLRAGAGRTLTCYVGKKGFEHADD
                     AVIDWRAPLAALFYRGKPGEASYTGPAGAVHGRLQLKRNLDIQARELVTIADDFDVRE
                     MRPGVAEPGEFLRQKLAARRDPYLQEIIATIQAQQYDLIQADANQVLVTQGVAGSGKT
                     SVALHRLAYLLHPGNADGIEARRCIVFGPNRLFLSYIASVLPELDIGQVTQTTFSEWA
                     CEQLRLDSRHVYDVALEAILSPAAHARERNAEYRRSVLRNSHNMGRLLERYVERRRRV
                     AIPDNGPTYTGIGPLKLTVRLPREQIVEVHAQFAELPLNRHRERFVEALKARLTRAYD
                     ETIARRLDETPETSKQFATQAARLREEAAQLQRLAILTRSLDDVALKERNTAQSLDRG
                     AAGLLELATYYERRADAALMSIQRTRDEVYEANVWRQAVEQLAAQLATDVDRFWPPLS
                     AVSEYHALLADRDLLSELGRDLFSPGEIAALHRARPETGDRIDLNDLPAMHFLHILVE
                     GVEPRYDHIVVDEAQDVSRLQLETVRRFSRNGSLTILGDLPQSIHAHRGIAAWDELRQ
                     AFDGWRYSFHEINVSYRATREITTFANTVLQSLAASTRGVSLAQPFERRGEAPQLHHL
                     RHESELPRAIAGSVTHIRSHGYQNIAIITRTVDAGIALAVALYDHLDAFEMIESAEVE
                     YRGGLVLLPVHLAKGMEFEAALVVGVDETTYGAAEFDARLLYVAVTRALHVLHVFWIG
                     NISKHLEAAVLRSRDATGLDYLTT"
     gene            7247..8254
                     /locus_tag="AC162G_13100"
     CDS             7247..8254
                     /locus_tag="AC162G_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5076788.1"
                     /translation="MDTTIVHVESVVKTFAGGVRALDGISLAVEPGTIYGLLGPNGAG
                     KTTLIRVLATLLPPDEGIARVAGFDVRRDPDKVRAQIGLAGQYAAVDEFLTGRENVEM
                     VGRLYGLSHSEARQRAGQVIERIRLVGVADRPVKTYSGGMRRRLDLAASLVGRPQLLF
                     LDEPTAGLDPASRMDLWELIKELVDRGTTVLLTTQYLDEADRLADRIAVIDHGRLISE
                     GTGEELKNRVGGSVVEMSVSEEERTRTLEALRGVAAGQPTYDEQRRKVFLPAPDGSRT
                     LMEALRRLDGAGITPEDVALHKPTLDDVFLALTGHVATSGNGQYSPASAREGTGPETG
                     G"
     gene            8259..9092
                     /locus_tag="AC162G_13105"
     CDS             8259..9092
                     /locus_tag="AC162G_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004921166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5076789.1"
                     /translation="MTITTAASRQNSLERTVAGIAVITRRNLIRVVRLPQLLLFSTIQ
                     PVMFMLLFNYVFGGAIVLALPPAAAGSYVNWLMPGLLAQFALFSGGQTAAGLSEDLLK
                     GVIDRFRSLPMARSAVLAGRTVSDLIRSAFILALLVAVGFVIGFRPQTGLFGMLGALA
                     VALAFGYAWSWVMAMVGLMVRTTESIQSAVYLVIFPLAFASSVFVPTDTMPSWLQWFT
                     EHQPVSVVTNALRGLILGEGGLPAGQTVGGQVVLALAWSAAITGVFAPLAVRIYRRAV
                     S"
     gene            9150..9431
                     /locus_tag="AC162G_13110"
     CDS             9150..9431
                     /locus_tag="AC162G_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076790.1"
                     /translation="MARCDRNGIRTVPFAVKRDNPIDTSLITASCASRDAALKPVAIG
                     VAVLCGTHHFNELERGLHPTLSCSSPQSFGFAGQTSWAANRADAGSKYN"
     gene            9628..>10205
                     /locus_tag="AC162G_13115"
     CDS             9628..>10205
                     /locus_tag="AC162G_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076791.1"
                     /translation="MLAALSNLLITLVPGQLNRLLRRRPALLALDLAFGAALLAVSGG
                     WRSPYYLFVLSPLLAAAFFFQLRGALIATAAFVPMYGLAVWAATVIGAPPPDGLVLSL
                     AVVGFFLVAGLFGYAATLLARVRQAHDSVNQAHRDLEVIHELTLSLQSAADVNEVEER
                     VLEAVTGDLGFPRAIVALVDQNERVITAWLGKA"
BASE COUNT         1816 a         3245 c         3209 g         1935 t
ORIGIN      
        1 cgggttgaga ccggcgcgcc ggcaggcgtt gcggaatgtc ccttcgtgca ggtgcggcga
       61 gcaggcggcg accacgacgc gggtcagatc gtggtcttta atgtctttct cgatcagctc
      121 ctgccccggg ctggagcaca tgaacttgta atcgcgcgac acgacgacgc cgcgatgctt
      181 caagcgcttc tcggcccaat cgcgcacttc cgccacgtcg accatgccgg cgatgttgct
      241 gccgcagtgg catatgtaga cgccgatacg ctctccttga gacttcggaa gtctctcaaa
      301 gacttccgaa gtcttgactg gcgaagtcga tttgtcactc atcagctcac ctcctcatcg
      361 ccgctcgtcc cgtgcagtgg ctgcggcatg ggcaggctct tgtccttgcg gctgggcttg
      421 cgcggcggag cggctgcctc cggttccggc atctcgacgc cgatacggtg gttcagcact
      481 tcgccgacgc tcaccaactc cctgccgaag cccagctcac ccggatcctt gccgaaggca
      541 aggcccatca attgggtgaa gaacacgatc ggcatgcggt agttcgtatg gaagtgccgg
      601 ttggtctcgt tctgatacgc gtcgatgttc agttggcaca tcgggcagac ggtcgccatg
      661 agatcggcct cgtactgatc ggcggcggag atcaagcgcc ggatcagctc gaaggccgtc
      721 tcggggccga tttgcgtcat gtggccgccg cagcaatgcg tcttgagcgg gaaatcgatc
      781 acctccgcgc ccagcgcttt caggagccga tccagctcgt tcgggtactc gtggtcggac
      841 caacgtttct tgtaatcggg acgcggcacc atgcagccca ggtacggcgc gacgcgcagg
      901 ccggtgagcg gccgcacgac cttgcgcttc acctcgtcca gcccgatgtc gttgatgatc
      961 acgtcaagca ggtggcgtat ttccagtgag ccgggatcgt agtgcagtcc tcccgccgcc
     1021 agcgccacgt tgaccttctc gctgagcacc ggactctcga gcatgtagtg atcggccttc
     1081 gccaggttca ggtagcaggc gctgcacggc gcgacgacgg tgcgcgtgcc gtccgcctgc
     1141 ttcgccgcga gcgcgagatt gcgaccgatc agcgcgtagg cgggcgtcag gctgatgccc
     1201 aggtattccg tcgcgccaca gcaattccag tcgcgtatct cctcgaggtt caggcccagc
     1261 ggctcgctga tcgccatcag cgaatcgtag taggcccggg cgctgctttc catcgagcag
     1321 ccgggataga acagataatg gtcgttcatg ctgccgcctc cacttccttg gcgcgcgcca
     1381 ggatcgcctt gagctggtcg atgccttcga tgcggcgcgg cgtgaagtcc atgcgtttct
     1441 tgctgagcat gccgaagccc atgggcgcca tgccgggcag gcgcagcggg aagtgtttca
     1501 ggtaatgccg cgtcgccagg ccaaactcga aactgcgccc gtacttctcg accatgtcca
     1561 cgaaggtctg agagaagtcg gccgccgtct ccgttgcggc cttgtccttg tacagaccgg
     1621 cgcgaatcgc catgctcttg agcgtgtaca tgacgtccgc gatgtgcacg tcctgcgggc
     1681 agcgcaccac gcagtggtag cacgacacgc agatccacgg cgtgttgctg cgcagcgctg
     1741 cctcgcgcat gccggcgcgg atcatggcga acagcatgcg cggtgtatga tccatgtcgg
     1801 cggccggggg gcacgacccg ccgcacgtgc cgcactgaat gcacatttcc aggcgcgaca
     1861 cgcctgccgt tgccgcgctc acttcttcga gaaatcggag attgggattg gtcgggtcta
     1921 acgaaggttc tgcgtgcggg gtcaaggggc acctccttca agacacacgt gaaagagcag
     1981 acggaaattg agcatgccaa tgccgaaaga aaattgtgaa attttataca ataataacct
     2041 gacatttatc acctcttaca gtgatatgta tccttttgcc gcattacgaa tgtcacaacc
     2101 catcgaagcc gctacgcgct gatggctggc caagacaaac cgcgcccggc aggcagaggg
     2161 atgctctgcc agcaggcgcg gtctactgtc gcgttaatgg gttcgcccta ctcgtgttat
     2221 tcgcggatcg gtttggttgc ggcgcgcgaa gcgagtcgcg ctatcggtga gcaggtctaa
     2281 gccagaccgt gaaagccgtt ccctggccgg gctggctttc gacagagata cgcccctcca
     2341 tcctgtcgac gatttccttg cagatcgcca atcccagtcc ggtgcctggc gctccagacg
     2401 ccaaacctgc ttcacctcgg aagaagcgct taaacagatt gggcaactcc tcggcaggaa
     2461 tacctcggcc cgtatcgcgc accgtgaatg tgatccagtc tctatcgccc cacgggcgaa
     2521 tggcggtcga caggacgatg gagccaccgc gcggcgtgta gttgatcgcg ttggccatca
     2581 ggttcgacat tacctgaaca atccgcttgg gatcaccgac ggccaggggc aaatcgggat
     2641 cgagatggca atcgagcagt tggccccgat cggaggccat ggcggaccga tcgccgatca
     2701 actcggcggc aagatagttg atatgggtcg gctcgagatg aatcttcgcc gcgcccagat
     2761 caaggcgcga aagttcgagc aggtcttcga tgaggttatg gagccggccc gtttcgcggt
     2821 gcagggtctg gagatatgcg ccacgcttgt cgggccggcc atgctccaac aaactcaaat
     2881 aaagcttggt attggtcaaa ggtgtccgca gctcgtggct gacattcgag acaaactgat
     2941 cctttaagcg atcaacttcc ttgaggcgct cgttggcttc tgccaattca cgcgtgcgtt
     3001 cagccactcg ctgctcgagt tcgtcggcat ggcgctcaag ctcgccgtac agatttgcat
     3061 tctcgacagc caccgcggct tggtcggcga agacttggag tcgttcggcc cgtgaagctg
     3121 tgaagaatcc cggagtggca ctgtagaggt taagaaaacc aatcaccagg cctttcaagc
     3181 gaatcggtgt gccggcatac gaccgggcgt ggaggttctc cacgatgtcc atccactggg
     3241 ggtggccctg ggtgtcggag atggccagcg attggccggt ccgcatcatc tggcgcaggt
     3301 aaggcacttc ggccaccgct aaacgggcag cctgcagctt gggttccggc tgtgagccgg
     3361 tgttcccacg ccatcgcgcg atatgggcga ggccacccgc gaccaacatg atgtccgccg
     3421 aatcgtgtgg cacgacgcgg ccaacattgg cgagaatgcg atcgagcacc tcgtcaagat
     3481 tgagggtgct ggtgagtgca gcagccgtgt cgcgcaatgc ctcggcgagc gcccactgtt
     3541 cacgctcggc gttgaataac tgagcatgtt cgatggcaat ggcggcctgg ctggcgaaag
     3601 ccgacacgag cgtcatatcg atgccgcgaa aggcatctgc ttcagagtgc tgcacattca
     3661 tcacgccaat gaccgtgtcg ccgctgatca tgggcacata gagagccgac agcaggtgcc
     3721 gctcctcgcc gaccggagtg gcacgcccgt cggctgtcag cccctgtccg atttcgcgca
     3781 ggtcgacgat tcgcgcctgg cgcgtgcgaa tcgtgtcgct gaccggcccc ttgcccggcg
     3841 ggatgcgcgg aagctgcgtc ggatcgatct cctcaccatc cacgatggca aaaccgtaat
     3901 agatcgtttc cgtatgctga tcgaatagcg ccaccgccaa atgaggtgtg gccaggagtc
     3961 cctgtgcgat ctcacgaaac atgacccagt agatctcgcg caaatccagc gtggtcgcca
     4021 gtgtgcgccc gatctcgttg agcgccgcga gctggcgagt gcactgctcg aggcgggctt
     4081 cgagctggcg catccggtct tcgagcccct gatgcgcagg atgggtgcac agtgaatcgg
     4141 acatatacat aaatgatctg tcgccgtcat gcgacactgc cagatcaaac tctggggcaa
     4201 tgtcaacgga tgggcttatt attaacctta ttctatatgc tggctgttga tcaggaagat
     4261 gtacttgcgt actaaggcat tctaaatgct ggctgttgat caggaagatg tacttgcgta
     4321 ctaacgcaat gcgaactgac cccatctgac gctaaacacc gtgagggcga ggaaatttgt
     4381 gatgtaattc ttacaagaag ccgatcgggg tattccttct cgatttgcta ggtgattcgg
     4441 tgcatgataa acttttgttt cgctgatcga acgattactc tgcctatccg cgacgcacac
     4501 catgatatga acgcgcaaga ccatcccgag tatgcccaag agcacgcaca tctgcagcgc
     4561 gcgctggaga cgctcgcgtc acgcctggag gcgctggcgc aggccgacct catgacggcc
     4621 tacgacaacg ccgccgaagc cctgctcatg cagtaccgcg ccgagtacag ggccttgaaa
     4681 agcgtgtcgg attcgccgta cttcgcgcgg gtcgacttcg cgccgcatga cccttcgact
     4741 acgctcaggg caggcgcggg gcggacgctg acctgctacg tcggcaagaa aggattcgag
     4801 cacgccgacg atgcagtgat cgactggcgc gctccgctcg cggccctctt ttacaggggc
     4861 aagcccggcg aggcgagtta taccggcccg gccggcgcag tgcacggccg gctgcagctc
     4921 aaacgcaacc tcgacatcca ggcaagagaa ctcgtcacga tcgccgatga ctttgacgtt
     4981 cgcgaaatgc gcccgggcgt cgccgagccg ggtgaattcc ttcgccagaa gctggcggcc
     5041 cggcgcgatc cgtacctgca ggaaatcatc gccacgatcc aggcccagca gtatgacctc
     5101 atccaggcgg atgcgaacca ggtgctggtc acgcagggcg tggccgggag cggcaaaacg
     5161 tccgtcgcgc tgcaccggct cgcttacctg ctgcatccgg gcaacgccga cggcatcgaa
     5221 gccagacgtt gcatcgtctt cgggccgaac cgcctcttcc taagctacat tgcgtcggtg
     5281 ctgcccgagc tggacatcgg ccaggtgacg cagacgactt tctccgaatg ggcctgcgag
     5341 caactgagac tcgattcgcg ccacgtctac gacgtggcgc tcgaggcgat tctttctccc
     5401 gccgcacacg cccgcgagcg gaacgcagaa taccgccgca gcgttctgcg caactcgcac
     5461 aacatgggcc ggctcctgga gcggtacgtc gaacggcggc ggcgagtcgc gatccccgac
     5521 aacgggccga cctacacagg aatcggtccc ttgaagctga ccgtgcgcct gccgcgcgag
     5581 cagatcgtag aggtgcacgc ccaattcgca gagttgccgc tcaaccgcca ccgcgagcga
     5641 ttcgtcgagg cgctgaaggc acgcctaacg cgcgcgtacg atgagacgat cgcgcgccga
     5701 ctcgacgaga cgccggagac cagcaagcag ttcgccaccc aggccgcgcg cctccgcgag
     5761 gaagcggccc agttgcagcg gctggcgatc ctcacgcgca gcctggacga cgtggcgttg
     5821 aaggagcgaa acacagcgca gtcgctcgat cggggcgcgg ccggcttgct agaactggcg
     5881 acctactacg agcgccgggc cgacgcagcc ctgatgagca ttcagcgcac acgagacgaa
     5941 gtctacgagg cgaacgtctg gcggcaagcg gtggagcaac tggctgcaca gctcgccacc
     6001 gacgtcgacc gattctggcc gcccctgagt gcagtgtcgg aataccacgc gctgctggca
     6061 gaccgggact tgctctcgga gcttgggcgc gacctgttca gccccggcga gatcgccgca
     6121 ctgcaccggg cgcggcccga gaccggagat cgaatcgatc tgaacgatct gcccgccatg
     6181 cacttcctcc acatcctggt cgagggcgtc gagccgcgct acgatcacat cgtcgtcgac
     6241 gaggcgcaag acgtgtcgcg actgcaattg gagaccgtgc ggcgcttctc gcgcaacggc
     6301 tcgctcacga tcctgggcga ccttccgcaa agcatccacg cgcatcgagg catcgccgcg
     6361 tgggacgaat tgcgccaggc attcgacgga tggcgctaca gctttcacga gatcaatgtg
     6421 agctatcggg ccacgcgcga gatcacgacg ttcgccaata cggtgctgca gtcgctcgcc
     6481 gcatcgacgc gcggcgtcag tctggcccag ccgttcgagc ggcgcggcga agcgcctcaa
     6541 ctgcatcatc tgcgccacga atccgaattg ccccgggcga tcgccggcag cgtgacacac
     6601 atccgaagtc acggctacca gaacatcgcc atcatcacga ggaccgtcga cgcgggtatc
     6661 gctctggccg tcgcgctgta cgatcatctg gacgcgttcg agatgatcga atcggctgag
     6721 gtggagtacc ggggcgggct ggtgctgctg cccgtccatc tggcgaaagg catggagttc
     6781 gaggcggcgc tcgtcgtcgg cgtggacgag acgacctacg gcgcggctga gttcgacgcc
     6841 cgcctgctgt acgtggccgt cacccgggcc ttgcatgtgc tgcatgtgtt ctggatcgga
     6901 aacatcagca agcacctgga ggccgcagtc ttaaggagca gagacgccac tggccttgac
     6961 tacttgacca cttgaccatt tggccactag accactattc cccatcgcac ccgtagatct
     7021 tccgccttct gcgccaattt tccacctcct gtcgcatatg cgacatgtca cttccctttg
     7081 gtgattgacg gctgattcaa cgcgtgttac cctgactgtg caacactgaa gatgatatgc
     7141 atcaatatgt tgcttatttt ttttccacgg tgccgtggca gccggggtgg tgcgggggag
     7201 gtcggagttg aacactagcc actctcattc cagaccaggg gaaccgatgg acaccacaat
     7261 cgtgcatgtc gagagcgtcg ttaagacgtt cgcaggcggt gtgcgtgccc tcgacggaat
     7321 cagtctcgcc gtcgagcccg gcaccattta cggcctgctc ggaccgaatg gagccgggaa
     7381 gacaactctc atccgcgtgc ttgccacgct gctcccgccc gatgagggca tcgcacgcgt
     7441 cgccggtttc gacgttcgcc gggatccgga caaggtgcgt gctcagattg ggctggcggg
     7501 ccagtacgcc gcggtcgacg agttcctcac tggccgggag aacgtcgaaa tggtcggccg
     7561 gctgtacggg ttgtcgcaca gcgaggcgcg gcagcgcgcc gggcaggtga tcgagcggat
     7621 ccgcctggtg ggcgtcgccg accggccggt gaagacctat tcgggtggga tgcgccggag
     7681 actcgatctc gccgcgtccc tcgtcggtcg cccgcaactg ctcttcctcg acgagccgac
     7741 agccggcctc gacccggcca gccggatgga tctgtgggag ttgatcaagg agctggtcga
     7801 cagggggacg accgtcctgc tcacgaccca atacctcgac gaggccgacc gccttgccga
     7861 ccggatcgcg gtcatcgacc atggccggct gatcagcgag ggcaccggcg aggagctgaa
     7921 gaatcgggtc ggcggctcgg tggtcgagat gagcgtgtca gaagaggagc ggacccggac
     7981 gctggaggcg ctgcgcggcg tcgctgccgg gcagccgacc tacgacgagc agcggcggaa
     8041 ggttttcctg ccggcgccgg acggttcgag gacgctgatg gaggcgctgc gccggctgga
     8101 cggcgctggc atcacgcccg aggatgtggc gttgcataag cctaccctcg acgacgtgtt
     8161 tctcgcgctg accggacacg tcgccacctc cgggaacggt cagtatagcc ccgccagtgc
     8221 gcgtgagggc actggccccg aaacgggagg gtagcttgat gaccatcaca acagccgctt
     8281 cccgacagaa ttcgctggag cggactgtcg cgggcatcgc ggtcatcacc cggcgcaacc
     8341 tgatccgcgt tgtccgcctg ccccaactgc tgctgttctc gaccatccag ccggttatgt
     8401 ttatgctgct gttcaactac gtcttcggcg gggcgatcgt gcttgcgctc cccccggccg
     8461 cagccggcag ctatgtgaac tggctcatgc ccggcctgct ggcccagttt gcactcttca
     8521 gtggtgggca gacggcggcc ggcctgagcg aggacctctt gaagggggtc attgaccggt
     8581 tccggtcgtt gccgatggcg cgctcggcag ttctggccgg ccggacggtc tcagacctga
     8641 tccgctcggc cttcatcctg gcgctgctcg tggccgtcgg gttcgtcatc ggttttcgtc
     8701 cgcagacggg cctcttcggc atgctcggcg cgctggcggt tgccctggcc ttcggctacg
     8761 cctggtcgtg ggtcatggcg atggtgggcc taatggtgcg caccaccgag tccatccagt
     8821 cggcagtgta tctggtgatc ttcccactcg cgttcgcgag ttcggtgttc gttccgaccg
     8881 atacgatgcc gtcgtggctg cagtggttca ccgagcacca gcccgtcagc gtggtcacga
     8941 acgcgctgcg gggtctgatc ctcggcgagg gcggcctgcc agcgggtcag accgtcggcg
     9001 gacaggttgt cctggcgctc gcctggtcgg ccgcgattac gggggtcttc gcccccctcg
     9061 ccgtccgcat ctatcgccgg gctgtcagct aggcccggac agcagcagcg tacggaggta
     9121 catctcaatc gcctctgccg atcgcggccg tggccagatg cgatcggaat ggtatccgca
     9181 ccgtgccatt cgccgtgaag cgcgacaatc cgatcgacac atccttaatc accgcaagct
     9241 gcgcctcgcg cgacgcggca ctgaagcccg ttgccatcgg cgtggccgtg ttgtgtggca
     9301 cgcaccattt caacgagttg gagcgcgggc tgcaccccac cctctcttgc tcgtcgccgc
     9361 agtccttcgg ctttgcagga cagacttcgt gggcggccaa ccgcgcggac gcgggatcga
     9421 agtataatta gacggtagct cgttcaactg ccaggagatg aagcgacgaa tcaatcgcct
     9481 ctaccgaatt cagctcgcgc ctgggacgag gccgcacacc tccggcgtgt cttcagcctg
     9541 ctcgttctct atcgctggct gagcctcctc ccgccgctga tctttctctt cgtctcggcc
     9601 acgcgctggc acgaggtcgc cgggctgatg ctcgccgctc tcagcaacct gctcatcacg
     9661 ctcgtccccg gacagctcaa tcgactcctc cgccggcggc cggcgctgct cgcgctcgac
     9721 ctggcattcg gcgcggccct gctggctgtc agcggcggct ggcgcagccc gtactacctg
     9781 ttcgtgctca gcccgctgct cgctgccgcc ttcttcttcc agcttcgcgg cgcgctgatc
     9841 gcgacggctg ccttcgtacc gatgtacggc ctggccgtgt gggcggcgac ggtgatcggc
     9901 gcgccaccgc ccgacgggct ggtgctcagt ctggccgtcg tcggcttctt ccttgtcgcg
     9961 ggcctcttcg gctacgccgc gaccctcctg gcgcgcgtgc gccaggcgca cgacagcgtc
    10021 aatcaggcgc atcgggacct ggaggtgatc cacgagctta cgctctcgct gcaaagcgcc
    10081 gccgacgtca acgaggtcga ggaacgggtg ctcgaagccg tgaccggcga cctgggcttc
    10141 ccgcgcgcga tcgtcgcgct ggtcgatcag aacgagcgcg tcatcaccgc ctggttgggc
    10201 aaggc
//
LOCUS       DBNHXB010000325         7949 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_926983, whole genome shotgun sequence.
ACCESSION   DBNHXB010000325 DBNHXB010000000
VERSION     DBNHXB010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7949)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_926983"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="AC162G_13120"
     CDS             <1..390
                     /locus_tag="AC162G_13120"
                     /inference="COORDINATES: protein motif:HMM:NF014692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR5076792.1"
                     /translation="KFIRMRCSGKTDVRYLLVAFEQGADGVYVVACPIGNCHHVRGNE
                     RGRARLRRAKKILDEIGIDDERLDMFFMSGSQAHAFANAAQTMTERIRKLGPNPLKAK
                     YEGRFAGMRDEVADEDVGFRGRRFGKQ"
     gene            complement(419..556)
                     /locus_tag="AC162G_13125"
     CDS             complement(419..556)
                     /locus_tag="AC162G_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076793.1"
                     /translation="MSIDELDFEGYEDSFPLWLGTATGNEQGQYHQACDQAFCQTSSV
                     V"
     gene            complement(1078..1491)
                     /locus_tag="AC162G_13130"
     CDS             complement(1078..1491)
                     /locus_tag="AC162G_13130"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR5076794.1"
                     /translation="MAAELLLDTGAFVALVDRSELRHVDCVTVLENWSGPIVTTEAVL
                     TETLYLVGPAWQAQQVCLEFLIRGAFLLIPSSRNSLQRVAALMKKYRDVPMDFADATL
                     VALGEDLQTDQVFTLDRRGFSVYRLPGKKPFHLFP"
     gene            complement(1481..1729)
                     /locus_tag="AC162G_13135"
     CDS             complement(1481..1729)
                     /locus_tag="AC162G_13135"
                     /inference="COORDINATES: protein motif:HMM:NF013561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HIR5076795.1"
                     /translation="MEHQLTLRLSADLAGRLDRAARRLARKRSEVVRLALEQFLSTEV
                     EARPIEQVRDLIGRVESGLPDLGQRHREYLVRRLRDGR"
     gene            1774..6492
                     /locus_tag="AC162G_13140"
     CDS             1774..6492
                     /locus_tag="AC162G_13140"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5076796.1"
                     /translation="MSNSPTRHRSGNDTKRPPIGAVMVVGGGIAGMQASLELAEQGFK
                     VYLVEAQSAIGGKMAQLDKTFPTNDCAMCTISPKLVETGRHPNITVMTNTEVLDVKGQ
                     AGDFSVRVRRNPRYVDLSKCIGCGDCAKVCPVIVPDTFNEGLAPRHAVYKLYPQAVPN
                     AYTIEKRGIAPCRDACPAGQRAQGYIALIREGRWHDALRVIKMDNPFPGICGRICNHR
                     CETACNRGLVDEPINIRALKRFVTDKVYSEPRKPVQPAPRLHEERVAIIGAGPCGLTA
                     AQDLVLAGYGATVFEAMPVAGGMLRLGVPEYRLPAEIIEREVQDIVDLGVDLRLNHRI
                     DNLDDIFDEGFDAVLIAVGAHEGIRLPIPGADLDGVLINTHFLRDVRLGRYKDEGGRM
                     KDESSSFIPHPSSLGQRVLVLGGGNVAIDVARSAIRLGREVHMACLESGETIPAHPWE
                     VEAAKEEGVVIHEGRTFERIGGDENGHVVGVECQHAASFSFDDMGRLSVEKVPNSTHV
                     IPCDTVIFSVGQRAGLAFIPEDAGVGLTARKMIAVNPNTLAATREGVFAAGDSVSGTA
                     FVIEAVNSGHVAARSIIRYLQREQLEPPPRPELPVVRLSREEIDARTVRGQIRRQARV
                     PLPELPVAMRIDNFAEVEGGYDDESAQREAARCLACGICSECMSCTFACGRDAINHDD
                     VERFEDIGVGAIVLAPGYQIYNAHLSEEYGLGRYPNVVTALQYERMLSASGPTKGHVQ
                     RPADGASPKRVAFLQCVGSRDQSHDYCSSVCCMYAAKEAIMTVEHARAEARAGNGNGD
                     VTCQVFFMDTRAFSKGYEEYYRRAEKKYGVKYTRCRLSDVKEDPRTHNLVIRYAASDQ
                     SSVISNQSSVVSDQSVENRLITDHCSLITEEFDLVVLSVGMEIAESVQKLGRSLGIEL
                     DSYGFCHTTLFDPLQTSRAGIYVAGPFREPKDIPETVVDASGAAAAAARLLAPARHTL
                     AQAQAYPPERDVLSDEPRIGVFVCHCGSNIGGFLDVPGVAESARSLPGVVHAEDNLYT
                     CSQDTIAHIIDQVKTLGLNRVVVASCTPLTHEPLFQDAIRHAGLNPYLFEMANIRNQC
                     SWIHSSNWEAATSKAVSLVRMAVARAMELKPLKTAEVPVNNAALIVGGGAAGMTAALM
                     LADQGFPVHLVEREAVLGGNLRHLRYFVPSRGNGETPTPQEYLAQTVTRVQEHPLIKS
                     YLNTELIETGGFKGNFTSTLRSHGETFRVQHGATIVATGGVEYKGKEYEYGKDPRIVT
                     QLEFEALLSNLQSPISNLQSPDSVVMIQCVGPGERFCSRLCCTTALKNALKLKELNPE
                     AEVTVIYRDIRTYGFKERLYTEARRAGVRFIHYEFDRKPEVAIGHQPSAISVRVWEPV
                     LGRELELTPDLLVLSTPVVPPPGARDLATRLKVPVDMDGLFLEAHVKLRPVDFAAEGV
                     FMAGMAHYPKFLDETIAQAQAAASRAASILAQESMLTNARVAVVDPLKCVGCLTCVRI
                     CPYDVPKVRSQFTGVGNIVGAAYIEPAICHGCGTCASECPARAIQLMHYTDAQTLIKI
                     DALFSNQLTVISNQSAVARS"
     gene            6556..7008
                     /locus_tag="AC162G_13145"
     CDS             6556..7008
                     /locus_tag="AC162G_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:6760..6762,aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR5076797.1"
                     /translation="MSENGFEPEIVAFCCHYCAYAAADLAGSMRLEYPASIKVIELPC
                     SGKLDALYVLRAFEDGADGVMVAGULEGDCHYLEGNLNARRRVEYVRKMLDGIGLDGR
                     RIQMINVSSAMGGQFAWSAAEMTAEIKRLGPNPLRSEQNGERQGEADE"
     gene            7001..7588
                     /locus_tag="AC162G_13150"
     CDS             7001..7588
                     /locus_tag="AC162G_13150"
                     /inference="COORDINATES: protein motif:HMM:NF039354.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HIR5076798.1"
                     /translation="MSNQADLQPGLRQEAINILGRLVPSEREALLIKCWMSHDARWFM
                     AVAGEYDLQVTNRLNQIAAHELGKVEAQRIVRALGLPPVTTLDDYLLAQEIFIGFLGP
                     DLLDYRVSKVSDHVCQVLVRRCFAHDNAMRAGIADFFECGIFARVTGWLDALGLVYET
                     NPPLGKCLKAQGQECLYTITLREATEQTRTDAERG"
     gene            7596..>7949
                     /locus_tag="AC162G_13155"
     CDS             7596..>7949
                     /locus_tag="AC162G_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5076799.1"
                     /translation="MEKKPYILIVDDDPDILEGILTILESQPYRLATARDGKKCMEMI
                     AQETPDLLILDLLMPRMDGWGVIREMRSEPRYTGVPILVLTTVIEDASRRRYELETGM
                     AMDVQDYVQKPARPDD"
BASE COUNT         1649 a         2452 c         2451 g         1397 t
ORIGIN      
        1 aagttcatta ggatgcggtg cagcggcaag accgacgtgc gctatctgct ggtggccttc
       61 gagcaaggcg cggacggcgt gtacgtggtc gcctgcccga tcggcaactg ccatcacgtg
      121 cgcggaaacg agcgcgggcg cgcccgcttg cgccgcgcga agaagattct cgacgagatc
      181 gggatcgacg acgagcggct cgacatgttc ttcatgtccg gcagccaggc gcacgcgttc
      241 gcgaacgcag cccagacgat gacggagcgc atccgtaagc tcggcccgaa tccgctgaaa
      301 gcaaagtatg agggccgctt cgcggggatg agggatgaag tggccgacga agatgttggg
      361 ttccggggca gacgtttcgg caagcagtaa cagggagctg aagatggctc gcatacgttc
      421 aaacaacgga actcgtctga caaaatgcct gatcacaggc ttgatggtat tgaccttgct
      481 cgttgccggt tgcagttccc agccacagcg ggaaactgtc ctcgtaacct tcgaaatcca
      541 actcatcaat gctcaagcag actcctacag ctacggtgta gtgggtggtc aaggtacgac
      601 ggggtttgga actgtgaacg agacaatccg cgaatcctat tatgccgacg ttggagatga
      661 agtcttcgcg tgggtcaacg gttcccagtg gtcctcccag gaacccggcc atcaagttat
      721 ctgtcgtatc ttagtacatg acgaagagat ttaccttgat gcagagcaag gcacatcttc
      781 ccaggatact caagcccaat gccgcggccc ggtttacatc ccacctacac ctactcccga
      841 gggcggatga ctgcccagag taaaacaaag atgaaggtgg aggcagaagg gatgaagagg
      901 ctgtagacga tgacgggttc agaggcagac gctcaagtag aggataacga ccaagcaaag
      961 gagttgagac tcgcctgtag tagcgacttt cgtcgctggg ctgttcggac gactgacccc
     1021 actccgcttc gctacgaggg caagtgagtc gtcactacga aatcgtgatc tttgacgcta
     1081 cgggaaaaga tgaaagggct ttttaccggg cagacgatat accgagaacc cacgtcgatc
     1141 cagcgtgaaa acctggtcag tttgcaggtc ttcacccagg gcaacgagcg tggcgtcggc
     1201 aaaatccatc ggcacatcgc gatatttctt catcaacgcc gcgacgcgct gcaggctatt
     1261 tctagaagag ggaatcagca gaaaagcacc acgtatgagg aattcaagac acacttgctg
     1321 cgcttgccag gcaggcccga ccagatacaa cgtctcggtc aagaccgctt cggtggtgac
     1381 aatcggcccg gaccagtttt caaggacggt gacacaatcc acatgacgca actcactgcg
     1441 atctaccagc gcaacaaaag cgcctgtgtc aagcagcaat tcagcggcca tcgcgcaagc
     1501 gcctcaccag gtactcgcgg tgacgctggc ccaggtcggg caagccgctt tcgacgcggc
     1561 cgatcagatc gcgcacttgc tcgatcggcc gcgcttctac ctcggtgctc agaaactgtt
     1621 cgagtgccag gcgaacgact tccgagcgtt tacgcgccag gcgccgggcc gcacggtcaa
     1681 gccgcccagc cagatcggca gataagcgca atgtcaactg gtgttccatc gtttccactc
     1741 ctttgtgtta cattgtaatg caaggaattg cgaatgtcaa attcgcctac ccgtcatcga
     1801 tccggtaacg acacgaagag gccacccatc ggcgccgtca tggtcgtcgg gggcgggatc
     1861 gccggcatgc aggcctcgct cgagctggcc gagcagggct tcaaggtcta cctggtcgag
     1921 gcccagtccg ccatcggcgg caagatggcc cagctcgaca agaccttccc gacgaacgac
     1981 tgcgcgatgt gcaccatctc gcccaagctc gtcgagaccg ggcgccatcc gaatatcacg
     2041 gtcatgacga ataccgaagt cctcgatgtc aaaggacagg ccggggactt cagtgtacgc
     2101 gtccggcgca acccgcgcta tgtggacctc agcaagtgca tcggctgcgg cgactgcgcc
     2161 aaagtatgcc cggtcatcgt gcccgacacg ttcaacgaag ggttggcccc ccgccacgcc
     2221 gtctacaagc tctatccgca ggctgtcccc aacgcctaca ccatcgagaa acgcggcatc
     2281 gccccgtgcc gcgacgcgtg tcccgccggc cagcgcgcgc agggctacat cgcgctgatc
     2341 cgtgaaggcc gctggcacga tgcgctgcgc gtcatcaaga tggacaaccc cttccctgga
     2401 atctgcgggc gtatctgcaa ccatcgctgc gagacggcct gtaaccgtgg actggtcgac
     2461 gagccgatca acatccgcgc gctcaagcgc ttcgtcaccg acaaagtcta ctcagagcct
     2521 cgcaaacccg ttcagcccgc gccgcgcctg cacgaggagc gcgtcgcgat catcggcgcg
     2581 gggccgtgcg gcctgaccgc cgcgcaggac ctggtgctgg cgggctacgg cgcgaccgtg
     2641 ttcgaggcca tgcccgtcgc gggcggcatg ctgcgcctcg gcgtgcccga ataccggctg
     2701 ccggccgaga tcatcgagcg cgaggtgcag gacatcgtcg acctcggcgt cgacttgcgg
     2761 ttgaaccatc gcattgacaa cctcgacgac attttcgacg aaggcttcga cgccgtgctg
     2821 attgccgtcg gcgcgcacga gggcatccgc ctgccgatcc cgggtgcgga cctcgacggt
     2881 gtgctgatca acacacactt cttacgagac gtacggctag gtagatataa ggatgaaggc
     2941 ggaaggatga aggatgaatc ttcatctttc atccctcatc cttcatcctt aggacagcgc
     3001 gtcttggtcc tcggcggcgg caacgtcgcc atcgacgtgg ctcgcagcgc gatccgcctg
     3061 ggccgcgagg ttcacatggc ctgcctggaa agcggcgaga cgatcccggc gcacccgtgg
     3121 gaagtcgagg cggcgaagga agaaggcgtc gtcattcacg aggggcgcac attcgagcgc
     3181 atcggcggcg acgagaacgg ccacgtagtg ggtgtcgagt gccagcatgc cgcgtcgttc
     3241 agtttcgacg acatgggccg gctcagcgtc gagaaagtgc cgaatagcac gcacgtaatc
     3301 ccatgcgaca cggtgatctt ctccgtcggc cagcgcgcgg ggctggcgtt catcccggaa
     3361 gatgccggcg tgggcttgac cgcccgaaaa atgatcgccg tcaatcccaa cacgctggcc
     3421 gccacgcgcg agggcgtgtt cgcggcgggc gacagtgtct ccggcacggc gttcgtgatc
     3481 gaggcggtga atagcggcca cgtcgccgcc cgctcgatca tccgctactt gcagcgcgag
     3541 cagctcgagc cgccaccccg acctgagctt ccggtcgtgc gactctcgcg tgaggagatc
     3601 gacgcgcgca ccgtgcgcgg tcagatcagg cggcaggcac gcgtgccgct gcccgagctg
     3661 ccggtcgcca tgcgcatcga caacttcgcc gaagtcgaag gcggctacga cgacgaatcg
     3721 gcgcagcgcg aagcggcgcg ctgcctggcg tgcggcatct gctcggagtg catgtcgtgc
     3781 acgttcgcct gcgggcggga cgccatcaat cacgacgacg tcgaacgctt cgaagacatc
     3841 ggcgtcggcg ccatcgtcct ggctcccggc taccagatct acaacgcgca tctatcggag
     3901 gaatacggac tcgggcgcta cccgaatgtg gtgacggcgc tgcaatacga gcgcatgctg
     3961 tccgccagcg gcccgaccaa ggggcatgtg cagcgcccgg ccgacggcgc cagcccgaag
     4021 cgcgtcgctt tcctgcagtg cgtgggcagc cgcgaccaga gccacgacta ctgctcttcc
     4081 gtgtgctgca tgtacgcggc caaggaagcg atcatgaccg tcgagcacgc ccgggccgag
     4141 gcccgggccg ggaatggcaa cggcgacgtg acctgccagg tcttcttcat ggacacccgc
     4201 gccttcagca agggctacga agaatactat cgccgcgccg agaagaaata cggcgtgaag
     4261 tacacgcgct gccggctcag cgacgtcaag gaagacccgc gcacgcataa tctcgtcatc
     4321 cgctacgcag ccagtgatca gtcgtcagtg atcagtaatc agtcatcagt ggtcagtgat
     4381 cagtcagtgg agaacagact gatcactgat cactgttcac tgatcactga ggagttcgac
     4441 ctggtggtcc tgtcggtcgg aatggagatc gccgagtcgg tgcagaagct cggccgcagc
     4501 ctgggaatcg agctcgactc gtacggcttc tgccacacca cgctattcga tccgctgcag
     4561 accagccgcg cgggcatcta cgtcgcgggg ccgttccgcg agcctaaaga cattcccgaa
     4621 accgtggtcg acgcaagtgg ggcggcggcc gccgcagcgc ggcttctggc cccggcgcgc
     4681 catacgctgg cccaggctca ggcttacccg cctgaacgcg acgtgctaag tgacgagccg
     4741 cgcatcggcg tgttcgtctg ccattgcggg agcaacatcg gcggctttct cgacgtgccc
     4801 ggtgtggccg agtctgcacg ctcgctgccc ggcgtggttc acgccgagga taacctgtac
     4861 acctgctccc aggacacgat cgcgcacatc atcgaccagg tcaaaaccct gggcctgaat
     4921 cgcgtggtcg tcgcctcgtg cacaccgctc acacacgagc cgctgttcca ggacgccatc
     4981 cgccatgccg gcctcaatcc ctacctgttc gagatggcga acatccgcaa ccagtgctcg
     5041 tggatacatt cgagcaactg ggaagccgcc acatccaagg cggtgtcgct ggtgcgcatg
     5101 gccgtggcgc gggcgatgga actcaagccc ctcaagacgg ccgaggtgcc ggtgaataac
     5161 gccgcgctga tcgtcggcgg tggagcagcc gggatgacgg ctgcgctcat gctggccgat
     5221 caaggcttcc ccgttcacct ggtcgagcga gaagcggtgc tgggcggcaa cctgcgccac
     5281 ttgcggtact tcgtgccttc aaggggaaac ggagaaacgc caacaccgca agaatatctt
     5341 gcccagaccg taaccagggt gcaggagcat cctctgatca agagctacct gaacaccgag
     5401 ttgatcgaga cgggcggttt caaggggaac ttcacttcaa ccctgcgcag ccatggtgag
     5461 acattccgcg tgcagcacgg cgcgaccatc gtcgccaccg gcggcgtcga gtacaagggc
     5521 aaagagtacg agtacggcaa agacccgcgc atcgtcacac aactagagtt tgaggcgctg
     5581 ctctcgaacc tccaatctcc aatctccaat ctccaatctc cagactccgt cgtcatgatc
     5641 caatgcgtcg gaccgggaga gagattctgc agccgcctgt gctgcacgac agcgctcaag
     5701 aacgcgctca agctcaagga gctgaacccc gaggccgagg tgaccgttat ctaccgcgac
     5761 atccgcactt acggcttcaa agagcggctg tacacggaag cgcggcgggc gggagtgagg
     5821 ttcatccatt acgaatttga ccgcaaaccc gaggtagcca tcggccatca gccatcggcc
     5881 atcagcgttc gggtgtggga gccggtgctg ggccgggaat tggagctgac gcccgacttg
     5941 ctggtgctga gcacgccagt ggtcccgccg ccgggcgcgc gggatttggc gacgcggctg
     6001 aaggtgccgg tggacatgga cgggttattc ctcgaagcgc acgtcaagct gcggccggtc
     6061 gacttcgccg ccgagggcgt gttcatggcc ggcatggcgc actaccccaa gttcctggac
     6121 gagacgatcg cccaagccca ggctgccgcg tcgcgggccg ccagcatcct cgcgcaggag
     6181 tcgatgctga ccaacgcgcg cgtggccgtc gtcgatccgc tcaagtgcgt cggctgcctc
     6241 acctgcgtgc gcatctgccc ctacgacgtg ccgaaggttc gcagccagtt caccggcgtc
     6301 gggaacattg tgggcgcggc gtacatcgag ccggctatct gtcacggctg cggcacgtgc
     6361 gcctcggagt gcccggcgcg cgccatccag ttgatgcatt acaccgatgc gcagacgttg
     6421 atcaagatcg atgcgttgtt cagtaatcag ttgacagtga tcagtaatca gtcagcagta
     6481 gccagatcct gacagtcaca actgatcact gaccactgat gactgatgac tgaccactga
     6541 ctaacggaga ctaccttgag cgaaaatgga ttcgaacccg agattgtcgc cttctgctgc
     6601 cattactgcg cctacgccgc cgccgatctg gccggctcga tgcggttgga gtatcccgct
     6661 tcgatcaagg tgatcgaatt gccctgctcc ggcaagctcg acgcgctgta cgtgctgcgc
     6721 gcgttcgagg acggcgccga tggggtgatg gtggcaggct gactggaggg cgattgccat
     6781 tacctggaag gtaatctcaa cgcccgaagg cgagtcgaat acgtgaggaa gatgctggat
     6841 ggaatcggct tggatggccg gcgcatccag atgatcaacg tttcttcggc catgggcggc
     6901 cagtttgcat ggtcggcagc cgaaatgacg gccgagatca aacgtctggg gccaaatcct
     6961 ttgcgcagtg agcaaaacgg cgagcgacag ggagaagccg atgagtaatc aggcagatct
     7021 acaacccggc ttgaggcaag aagccatcaa catcctgggc cgacttgtgc cttcagaacg
     7081 cgaagcgctg ctcatcaaat gctggatgtc acacgatgcg cggtggttca tggcggttgc
     7141 cggagaatac gacctgcagg tcacgaaccg cctcaatcaa atcgccgcac atgagctcgg
     7201 caaggtggag gcccaacgca ttgttcgtgc attggggctg ccacccgtga cgactctcga
     7261 cgactatctc ttggcgcaag aaatcttcat cggctttctt gggcccgatc tgcttgacta
     7321 ccgcgtgagt aaggtaagcg accatgtatg tcaggtgctt gtgcggcgct gcttcgctca
     7381 cgataatgca atgcgcgcag gtatcgccga cttctttgag tgtggcatct tcgcacgagt
     7441 caccggctgg ctcgacgctt taggtctggt gtacgagaca aacccgccct tggggaaatg
     7501 cttaaaggcc caagggcagg aatgcctcta cacgatcact ctcagagagg caacagaaca
     7561 gacccgcact gatgcggaga gagggtgaag tcttgatgga aaagaaaccc tacatcctga
     7621 tcgtcgacga cgaccccgac attctcgagg gcatcctcac catcctggag agccagccgt
     7681 accgtctggc caccgcacga gacgggaaaa agtgcatgga gatgatcgcg caggagaccc
     7741 cggacctctt aatcctggac ctgctcatgc cgcgcatgga cggctggggc gtgatccgcg
     7801 agatgcgcag cgagccgcgc tacaccggcg tgccgatcct cgtgctcacg accgtcatcg
     7861 aggatgcgag ccgccgccgc tacgaattgg agaccggcat ggccatggac gtgcaggatt
     7921 acgtccagaa gccggcccgg ccagacgat
//
LOCUS       DBNHXB010000326        15832 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_424405, whole genome shotgun sequence.
ACCESSION   DBNHXB010000326 DBNHXB010000000
VERSION     DBNHXB010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 15832)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15832)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15832
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_424405"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="AC162G_13160"
     CDS             <1..324
                     /locus_tag="AC162G_13160"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR5076800.1"
                     /translation="YTWLHICGNTTDKLESIAETGASCFSLDYKVNLAEAKQKIGGRV
                     ALAGNIDPVSILNQKGPDDVRAAARACIEAAAEGGGFVLTSGCDLPPTISMENLQAML
                     SAGVM"
     gene            complement(449..889)
                     /locus_tag="AC162G_13165"
     CDS             complement(449..889)
                     /locus_tag="AC162G_13165"
                     /inference="COORDINATES: protein motif:HMM:NF014470.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR5076801.1"
                     /translation="MDLFGIGSSELIVLLLLAGVLLGPRRLARLARDAGKLLRQIQAM
                     TRDLTKQLNREIDLLEATEGKSGSSSPEAEGHRETKLPEAYRRFREDFPDEGKLDNLS
                     RGSTRGNRQSSREQPPVAMPTMAPTGEAQSENASPSEPKSSTVV"
     gene            complement(898..1641)
                     /gene="tatC"
                     /locus_tag="AC162G_13170"
     CDS             complement(898..1641)
                     /gene="tatC"
                     /locus_tag="AC162G_13170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR5076802.1"
                     /translation="MTYLLGFGRVRRRTAAAATPEAMTVLEHLEELRTRIFKAGIALA
                     VTTTLSFAFATQLVDFLTAPIGGRAALVSIEVTENISAFVRVALLAGVVLGLPFLLYQ
                     AIAYIAPGLTLRERRWLYLFIPAAMVFFVSGVAFAWFVMVPTALPFLINFLGIPNHPR
                     PLNYIGFVTSLLFWIGMSFEMPLVIYFMAKLKWVTAGQLIRSWRYAFMGIATAAAVIT
                     PTADPLNMGLVMVPLLVLYLISIALARIA"
     gene            complement(1729..1920)
                     /locus_tag="AC162G_13175"
     CDS             complement(1729..1920)
                     /locus_tag="AC162G_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR5076803.1"
                     /translation="MPIRIGTPELIILLIIVLLLFGVGRISRVARELGQGIRELRRGL
                     SEENEGSEKKQTEDESEAR"
     gene            complement(1939..2541)
                     /locus_tag="AC162G_13180"
     CDS             complement(1939..2541)
                     /locus_tag="AC162G_13180"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5076804.1"
                     /translation="MAIVLAAGLSTRMGQSKPLLPFGERTVIEHILSVLRECSVGAIR
                     VVTGYECEAVERCLMDWPVQMVFNPNYATGEMLTSLQIGLRSASGEVDAALIVLGDQP
                     ALERSVVETIVATYRSGQGSIVVPSYRMRRGHPLLIDRKHWTDILALGEGQTLRDFFR
                     SAAEAIHHVEVTTPSVLRDMDTPADYRRELADYLSRHPTH"
     gene            complement(2564..3745)
                     /locus_tag="AC162G_13185"
     CDS             complement(2564..3745)
                     /locus_tag="AC162G_13185"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR5076805.1"
                     /translation="MNEFVSVVSALMKAVERGENVALASIVRVRGSSPRHNGACMLIW
                     PDGRIAGTIGGGTLEWRVVEHARAALAEREPRFSNYVFDTRGGPDSVGLCGGSVDVHI
                     DVAVSEFAAVARVSLEALARGETVALASIVREGNSAPRRNQRRMLVWPDGRTFGSLSR
                     EVPHDRVVEDARAALMEDKPRLANYAVSPDRGPENVLVHLDILRPEPTLLIVGAGHVA
                     QPLAMIGSMMGMRVCVLDDRAEWANRERFPTAAEIAVVGYEPIHEILDPIPFPMTPAT
                     YVVITTWGYDLPAMEQALKQNPAYIGLVASPTKARVLFKRLRQSGFPDEAIQRIRAPV
                     GLDLGAENPAEIAVSIMAEVLATMRGKSSLPLREVRGARIDALFARPLVDVTPATEAV
                     A"
     gene            complement(3784..4017)
                     /locus_tag="AC162G_13190"
     CDS             complement(3784..4017)
                     /locus_tag="AC162G_13190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR5076806.1"
                     /translation="MPTYEYLCQACSTVYEERRTFARADDPSRCPECDSRRVRRLLST
                     FYAFSPGSSLSLGGGCACSAGGTCACRATPARS"
     gene            complement(4037..5044)
                     /locus_tag="AC162G_13195"
     CDS             complement(4037..5044)
                     /locus_tag="AC162G_13195"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR5076807.1"
                     /translation="MKRYTLALFILFSWLFLIGAAGAQPPPQDGWPPQSTLFNDALFL
                     ARVVPFLIALGIAVGVVQAREAARRGGDAILGDQVRRHDTGTILAHWMNAFGLILGFV
                     TGAMILRWTGRAIELRMAFILHYVGAALALFALFNHLTRHGVSGGTGLIPKRLGVIRD
                     LIGELLEYTGLFGPEGAVLRIRWPKAIRQPIARYARALLGYRPHEHGKYLATEQTLSY
                     PPWAILIGLITVTGLIKVLRYIYEIPSSIVATATAIHDLAAIAIGVMLVIHLLPLLLV
                     PANWPLLLSMFRTTVPRKYVEERHPIWYKALRGTKAPEADRETSPERRAAQMASVPSE
                     D"
     gene            complement(5063..6208)
                     /locus_tag="AC162G_13200"
     CDS             complement(5063..6208)
                     /locus_tag="AC162G_13200"
                     /inference="COORDINATES: protein motif:HMM:NF013123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HIR5076808.1"
                     /translation="MGNHHMTRNRISSVGLVLATVLVLSVAGVVVAQAQLPGIRLTND
                     PANDLRPAWSPDGTKLAFFSNRSGSNDIWVMDADGRNQRQLMNDSADDRRPVWSPDGA
                     WIAFDSDRAGTRDLWIVDTEGGNLRQLTSDPGQETFPAWSPDGSQIAYYSFAGGVLDL
                     WVVDVEGKNPRPVMTDLADERLNQCTFACHTPAWSPDSTQIAYPAMNQTQIWVVGTDG
                     KGSHALATGDMRVHYPSWTPDGKILFLSEYRNDRQEPVNDVWVMDADGTNGSVLYAAI
                     PHGGPLYWSPGGGSLIAFHSPRAGNFDIYTTVLGQEAPAETAAPAPATAAPVEQATAV
                     ADVAAETAPASETTGPSNEVLVGAVVAVMAAAGVLVAVYLARRGRPV"
     gene            complement(6227..7000)
                     /locus_tag="AC162G_13205"
     CDS             complement(6227..7000)
                     /locus_tag="AC162G_13205"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5076809.1"
                     /translation="MAQEEIRPNENTSQGAAPLVVSRRDFLKQAGAGFVAVAAIGVGG
                     VNLLNDPGPAELQLSSGVIMPDPTLCIGCLTCEVACSRAHREAGLSDIPRIRIFYDEN
                     TSVDPVITTAYGDRGQYHQSPCLMCPDAPCHHVCPANALPVEPTSGARIIDETKCIAC
                     GRCAEACPFPTFSETEATGREVLGQRTRITYDPSKNVYTKCDLCYWRPEGPACVEACP
                     VNIRILQGIIQSDRLCLDAPPATREHWEQQSEMDWAINL"
     gene            complement(7158..7628)
                     /locus_tag="AC162G_13210"
     CDS             complement(7158..7628)
                     /locus_tag="AC162G_13210"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR5076810.1"
                     /translation="MNVQDGLLAIPYGVYVITTGGESKQPGAFTASWLMQVSFEPLLL
                     ALAVDKTSYSQGLLEEQRVFAVNFLGQAQTPLAARLGTPHHIQPHKFASVSWHSGITG
                     VPVLDEALAYLECEVSDTLDPGGDHVIYVGKVVAGEVRHREPTLTLERSGLRYR"
     gene            complement(7676..8086)
                     /locus_tag="AC162G_13215"
     CDS             complement(7676..8086)
                     /locus_tag="AC162G_13215"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIR5076811.1"
                     /translation="MDDQVPTPRLVKKAAVAYDLNALPPALIRSYSILRRKLERAGFR
                     IEVVMRPLSQLPPDVDVLFVADELVESARQILPEAQVMLLDLAQVHQPAFDELLQQLN
                     SGQALYALRVEEGDDQADASRRVVVRYRGNEKLY"
     gene            complement(8162..8425)
                     /locus_tag="AC162G_13220"
     CDS             complement(8162..8425)
                     /locus_tag="AC162G_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HIR5076812.1"
                     /translation="MEITVGDYYFYPQVVTVTVGTEVTWEPVGVLYHTIVSKDSPPVF
                     SGATGGVGTRAFKFTFRKPGVFAYHCDYHPGAMNAWVMVVEDD"
     gene            complement(8755..9597)
                     /locus_tag="AC162G_13225"
     CDS             complement(8755..9597)
                     /locus_tag="AC162G_13225"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR5076813.1"
                     /translation="MNNDTSRVLETAILAARTGGQLAVTRLGKPGYEKRKGPRDVVAG
                     AVIDVQKRIVDVISQEFPEAHFLLEESDAVQDTQADPLWIVDPVDGSLNFLHGIPIFA
                     VSIAYRVAGLYRVGVVYDPCRDELFHAVTGSGAFLNGHPIHVDLFSDGREAWEQAVVG
                     TDWRGNDDELRKALKLARYVAGETFQLITLGSPALGLCYVAAGRLHAYYGLDALKLWD
                     VAAGAVILVEAGGIFTSIEGAAWQHAQGGYIASNNVVHGWLNRTAGAILGLYRVDEQL
                     QRAV"
     gene            complement(9590..10027)
                     /locus_tag="AC162G_13230"
     CDS             complement(9590..10027)
                     /locus_tag="AC162G_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076814.1"
                     /translation="MRVALRIGLVVSVVMMVAGCGSPSAATEAPTEAPPTQASSTPAS
                     TQAAPPTEAPTEVPTEPPPTAEPTAATPVETVAFNWAPGSVPPSDADDVAVIAVDLME
                     NDAIVSVTGGETVLNVSYDPTRITVEEIMALLRQMGHPVVVNE"
     gene            complement(10143..10526)
                     /locus_tag="AC162G_13235"
     CDS             complement(10143..10526)
                     /locus_tag="AC162G_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076815.1"
                     /translation="MIPATLKCEATQQWTQTQSDLTLSLCFEPYPPRLGVLTTYQAAL
                     IDAAGQPVVDATVELTMVGGMGGMEGEHDEDFSVTLASQGAGLYTAEGRVGPTDLVLT
                     EVLVVIRHGGQSRTFSIPADDLSPP"
     gene            10525..10764
                     /locus_tag="AC162G_13240"
     CDS             10525..10764
                     /locus_tag="AC162G_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076816.1"
                     /translation="MDEAGEAVTLELAGTVGDGTGLVSVGSAVKLGAAVVELAPVVGV
                     AVSTATDGRQPTMQTLTSNKPTIHLTVCTRWSFMP"
     gene            complement(10941..11933)
                     /locus_tag="AC162G_13245"
     CDS             complement(10941..11933)
                     /locus_tag="AC162G_13245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR5076817.1"
                     /translation="MKAFELYDATTVEEAVGLLEKYGAKSKVVAGGSDLVTGVMKDWV
                     QGPGMPYPEVLIDITTIPELHGIAKEGADLKIGAATTLTDVVESEEIRTGWSVLSDAA
                     FSVASPLIRNFGTLGGNINQRPRCWFLRGESFPCYKKGGDFCFAVTGDNEPHAIIGGE
                     LCYIVHPSDTATALLALKARTTIASPSGRREVAFDDYFIGPREDVLRENVLQANELMV
                     DVKVPPPAANTKMAWIKLKDRQVYDFALTSVAVVATLDGGVWQDGRIVLGGVAPVPWR
                     AVVVEQALVGKDVKSAIKSAAALIRGEARPLSNNGYKVDLAIGLTERAVLSLVA"
     gene            complement(11966..14470)
                     /locus_tag="AC162G_13250"
     CDS             complement(11966..14470)
                     /locus_tag="AC162G_13250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR5076818.1"
                     /translation="MSVKARIDPIRALGDPAYAQSLSPAQLRELARDPVWLDLVADEP
                     ELMTALNARLGLKGVSRKAKVPPWQEESPSFSVVGTRQKRVHGQGVVSHTGVYLENTP
                     PLPNMAYMKLLRSPHPHAKILSIDTSKAEAMPGVVAVLHRFNLPEEYLESRVGGANPS
                     RYLFAEEVFQVGAPVVAVAAIEEHIADEAIRLIEVEYEVLPAVLNFREAALPSTSKQW
                     ENEFDGTIIGIPNPFLRGEGKTALDQAEVVVESVSRSPFHQHAALELSNILTYWDNDQ
                     LIHYRTTRHPHGDRNSLAQLLGIPANKVRVIQQGYMGASYGNLRSVDQEEGIASILAR
                     VANRPIRYIATRYEDFIYRTGRGEQETTSRIGVNRDGKMVAAHFSTLGNVGAVRGGKV
                     TGGWVGFEQSYAFPNLELDGTDVMTNSFRNGTLRCVSHPTATLAQEIAVDKAAYAIGM
                     NPLDIRLQNINELGNLDDGTPYSNPGLRDALEQAAERIGWAEKWHAPGANEVRPGVFH
                     GIGIACHTCSHGAGGHPSTATIAVYNNGRVDVISAATEVGSGQRTAMAMIAAEALGVP
                     LSAVNISVGVDSAMTVDTGVTAGSRQINSGGWGVYEAAIDAKNQILAGGAQAFVDAAA
                     AEDPPREIEVTPDQLDIRDGLVVFKDNPEVSMEIGDAVDAVVPNTAVIGRGAHFHPGT
                     WERIHMAAQAAEVEVDTVTGTVTVLKYVAAHDVGRAINRMGTEQQVEGGVIMGIGAAL
                     TEELKVDFATGLPLNGNLLDYKVLSIKDVPRTIDVILVEKPKEWGVFGAQGTGEPPIA
                     LPPPTIANAVYNAIGVWIEDMPISRRKVLEALKRAA"
     gene            complement(14488..15195)
                     /locus_tag="AC162G_13255"
     CDS             complement(14488..15195)
                     /locus_tag="AC162G_13255"
                     /inference="COORDINATES: protein motif:HMM:NF013923.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5076819.1"
                     /translation="MADEEKVPVEEPESAPLKISRRDFLVGAGAGVVVTGAAATAVIA
                     LRQPETVEVIKEVQVPGETVTVGTTLPATMRAVTLNIDGRAHEVTVDVRESLWETMTK
                     KLGLGTAINLGCDRAQCGACAVVVDGRAVNGCTILSARLGRGQQIVTVAGLATGVGEA
                     DLHPIQKAFWEEGGWQCGICTRGFIMSTYALLSKNLNPTETDVREALAGNICRCSEYP
                     MIFNSVFKAAELMRAAS"
BASE COUNT         3074 a         5049 c         4688 g         3021 t
ORIGIN      
        1 tacacctggc tgcacatctg cggcaacacg accgacaaac tggaatcgat cgccgaaacg
       61 ggcgcgagct gtttcagcct ggactacaaa gtgaacctgg ctgaagccaa gcagaagatc
      121 ggcggacgag tcgcactggc cggcaacatc gaccccgtgt cgatcctcaa ccagaaaggc
      181 ccggacgacg tgcgcgctgc ggcgcgagcc tgcatcgaag cggccgcgga gggtggtggg
      241 ttcgttttga cgagcgggtg cgacttgccg ccgaccatct cgatggaaaa tctgcaggcc
      301 atgctgtccg ctggggtgat gtgaggttgc agttattcaa cgagtattac ggtcagccga
      361 ccgatcgggc caatcaccac cgcacgccat tcagcggcgg cagcggtgtg ctgatcagag
      421 ggagattgaa gcccggcgtg atccgagcct agacaaccgt cgaggatttt ggctcgctgg
      481 ggctggcgtt ctctgactgg gcctcaccgg tcggcgccat ggttggcata gccaccggcg
      541 gctgctcccg gctagactgc cggttgccgc gggtagatcc acgagacaag ttgtccagct
      601 tgccttcgtc gggaaagtct tctcgaaaac gacgatatgc ctccggcaac ttggtctccc
      661 ggtgcccctc cgcctctgga ctggatgagc ctgatttacc ttcggttgct tcgagcaaat
      721 caatttcgcg gttcaactgc ttggtgaggt cgcgggtcat ggcctggatt tggcgcagga
      781 gttttccagc gtcgcgcgcc aacctcgcca gtcgcctggg gccgagcagc accccagcca
      841 ggagcagaag cacgatcagt tcagagctgc cgatgccaaa cagatccatg tggccctcta
      901 agcgatccgc gccagcgcga tgctgatgag atacagcacc aacagcggca ccataaccaa
      961 acccatgttc agcggatccg ctgtgggtgt gatgacagca gcggcggttg cgatccccat
     1021 gaaagcgtag cgccagctac ggatcaattg gccggccgtc acccatttca acttcgccat
     1081 aaagtaaatt acgagcggca tctcgaaact cataccgatc cagaatagga gcgacgtgac
     1141 gaaaccgatg tagtttagcg gacgggggtg attggggatg ccgaggaagt ttatcaggaa
     1201 aggtaatgcg gtcggaacca tcacaaacca ggcgaacgct accccgctca cgaagaagac
     1261 catcgccgcc gggatgaaca agtacagcca gcgacgctca cgcagggtga gcccaggcgc
     1321 tatataggcg atggcctgat acagcaggaa tggtaaaccg aggacgacac ccgccagcag
     1381 cgccacgcgc acgaaggcgc tgatattctc ggtaacctcg atggacacca gcgccgctcg
     1441 cccgccgatc ggggccgtga ggaaatccac gagctgtgtc gcgaaggcga agctgagggt
     1501 cgtggtgact gccagagcga tacctgcttt aaagatccgt gtccgcagtt cctccagatg
     1561 ctcgaggacc gtcatcgcct cgggggtagc agcggcagcc gtccggcggc gcacacgccc
     1621 aaaccccagc agataagtca atacggaccg ccgcttgcgc gttcgttcgc caggagcatt
     1681 gggaagcgca ggggtagcac ccatagtctc gttctgtcac atttaagctt atcgtgcctc
     1741 agactcgtct tcggtttgct ttttttccga gccttcgttc tcttccgaaa ggccgcgcct
     1801 cagctcgcga ataccctggc cgagttccct ggcaactcgg ctaatacggc ccacgccgaa
     1861 gagcagcagg acgatgatca acaggatgat cagctcaggc gttccgattc gaatgggcat
     1921 gtcacacctc tagtgggttc agtgagtcgg atgacgactc aagtagtcgg ccagttcacg
     1981 ccgataatcg gccggggtat ccatatcccg caacacactt ggagtagtca cctcgacgtg
     2041 atggatagcc tcggcggcgc tccgaaaaaa atcgcgcagt gtttgtccct cacccagagc
     2101 cagaatgtcc gtccagtgtt tccggtcgat aagcaaggga tgcccgcgtc gcatccggta
     2161 agagggtacg acgatactcc cttgcccgct gcggtaggta gcgacgatcg tttcgacgac
     2221 cgagcgctcc agcgcgggtt gatcacccaa cacgatgagc gccgcatcga cctcgcccga
     2281 agccgatcgg agcccgatct gtagcgaggt cagcatctcc cccgtcgcat agttaggatt
     2341 gaacaccatc tgcaccggcc agtccatcag gcaacgttcg acggcctcgc attcgtagcc
     2401 cgtaacgacc cggatcgccc cgaccgaaca ctctcgcaga acggacagaa tatgctcgat
     2461 gaccgtccgc tcgccgaagg gcaacaacgg cttggattga cccatccgtg tcgagagccc
     2521 tgcggccagc acgatcgcca tgatcatagg ctgtgcctag gggtcaggcc accgcttcag
     2581 tcgccggggt gacatcaacc agcggccggg cgaacagggc gtcaatccgg gcgccacgaa
     2641 cctccctgag aggaaggctg ctcttgcctc tcatcgtggc caacacttca gccatgatcg
     2701 acacagcgat ctccgccggg ttttccgcgc cgaggtccag gccaaccggc gcccggattc
     2761 gctggatggc ttcatcaggg aaaccggact ggcgcaagcg cttgaacagc accctggcct
     2821 tggtcgggct tgcgaccagc ccgatatacg ctggattctg cttcagcgcc tgctccatgg
     2881 cgggcaggtc gtaaccccag gtggtgatca ccacataggt cgccggcgtc atggggaatg
     2941 ggatcggatc gagtatctcg tggataggct cgtagccgac gaccgcgatc tcggctgcag
     3001 ttgggaatcg ctcgcgattg gcccactccg cgcggtcgtc taagacgcac acccgcatcc
     3061 ccatcatgct cccaatcata gccagcggtt gggcgacgtg ccccgcgccg acgatcagca
     3121 gcgtcggttc gggccgcaga atgtccagat ggacgagcac gttctcggga ccgcgatccg
     3181 gggaaacggc gtagttggca agacgcggct tgtcttccat caatgctgcg cgcgcgtctt
     3241 cgaccacacg gtcgtgaggc acctcacgac tgaggctgcc aaatgttcgg ccgtcaggcc
     3301 agaccaacat gcgcctctga ttacgccgcg gcgccgagtt gccctcgcgc acgatagacg
     3361 ccaacgccac ggtttcgccg cgtgccagtg cttccaatga cacacgggcc accgccgcaa
     3421 attcactcac ggctacgtcg atgtgtacgt ccacactgcc gccgcacagt cccacgctgt
     3481 cggggccgcc gcgggtgtcg aacacgtagt tcgaaaaacg cggctcacgt tcggcgagtg
     3541 ccgcgcgcgc atgttccacc acacgccatt ccaacgtccc gccgccgatg gtgcctgcga
     3601 tccggccgtc gggccagatc aacatgcagg ctccattgtg gcgtggtgag gaaccccgta
     3661 cccggacgat ggaagccagc gccacattct cgccacgttc cacagccttc atcaaggcag
     3721 agaccacgct cacgaattcg ttcatagccg ctatcctcag caggtgccta gattatacac
     3781 cgtctagctg cgcgctggcg tcgcacggca ggcacaggtg ccgcccgcgc tgcaggcgca
     3841 gccaccaccc agggacaggc tgctccccgg actgaatgcg taaaacgtcg agagcaagcg
     3901 ccggacgcgc cggctgtcgc attccggaca gcgcgacgga tcgtccgccc gggcaaacgt
     3961 ccgccgctct tcgtaaaccg ttgagcatgc ctggcacagg tattcgtagg tgggcattgc
     4021 gattttccct taaatattag tcctcgctcg ggacacttgc catttgtgct gctcgtcgct
     4081 ctgggctggt ttctctgtcc gcctcaggcg ccttcgtccc gcgcagtgcc ttataccaaa
     4141 tgggatgccg ctcttcgaca tactttcgag gcaccgtcgt tcggaacatg gacagcagca
     4201 gcggccagtt cgccggaacc agcagcaatg gcagcaagtg aatgacgagc atcacgccga
     4261 tggcgatcgc cgccaggtcg tggatcgccg ttgccgtagc cacgatactg ctcggaatct
     4321 catagatata tctcagtacc ttgatcagac cggtcaccgt gatcaggccg atcaggatcg
     4381 cccagggggg ataggataat gtttgttcgg tagccaggta cttgccatgt tcgtgcggcc
     4441 tgtaaccgag cagcgccctc gcatagcggg ctatcggctg ccgaatcgct ttcggccacc
     4501 gaatccgcag caccgctcct tccgggccaa acaggccggt gtattccagc agctcgccga
     4561 ttaggtcccg aatcacgccc aatcgcttgg gaatcaaacc tgtgccgccg ctgacgccat
     4621 gccgcgtcaa atgattgaag agggcgaaca gggcgagtgc cgcgccgacg tagtgaagga
     4681 tgaaggccat ccgcagctct atggcgcggc ctgtccagcg cagtatcatg gcgccagtga
     4741 cgaagccgag gatcaagccg aaagcattca tccagtgagc gaggatcgtc ccggtgtcgt
     4801 gccgccgcac ctgatcgccg aggatggcgt ccccgcctct gcgcgccgct tcccgggcct
     4861 ggacgacgcc caccgcgatg cccagggcga tgaggaaggg gacgactcga gccagaaaga
     4921 gcgcatcgtt gaacagggta ctctgcggtg gccagccgtc ctggggggga ggctgtgcgc
     4981 cagccgcgcc gatcagaaac agccaactga acaggataaa tagggccaac gtataacgtt
     5041 tcatgctacc tcctgtacgc agctacacgg gccggcctct acgggccagg tagacagcca
     5101 ccaatacccc ggccgcggcc atgactgcga caacggctcc gaccagcact tcgttcgagg
     5161 ggccggtggt ttccgaagcg ggcgcggtct cggctgccac atcggccaca gccgtcgctt
     5221 gctccactgg cgccgcggtg gccggtgcag gggctgccgt ctcagcaggc gcctcctgtc
     5281 cgagcacggt tgtgtagatg tcgaagttgc ctgcgcgcgg cgagtggaag gcgatcagcg
     5341 agccgccccc tggactccaa taaagcggcc cgccgtgggg aatagccgca tacagcacgc
     5401 tgccattcgt cccgtctgca tccatgaccc agacgtcgtt gaccggctct tgccgatcgt
     5461 tccggtactc ggacaggaac aggatcttcc catccggcgt ccacgagggg tagtgcacgc
     5521 gcatatcgcc ggtagcaagg gcatgtgaac ccttcccatc ggtcccgacc acccagatct
     5581 gcgtctgatt catcgccgga taggcaattt gtgtgctatc agggctccag gccggcgtgt
     5641 ggcaggcaaa ggtacactgg ttcaaccttt catcggccag atcagtcatg actggccgtg
     5701 ggttcttccc ctcgacatcc actacccaga ggtccaacac accccccgcg aaggaatagt
     5761 aagcgatctg agagccatct gggctccagg cggggaacgt ctcctgaccg ggatcactgg
     5821 tcagttgcct gagattccct ccctcggtat ccacgatcca caggtcacga gtaccggcgc
     5881 gatccgaatc gaaggcgatc caggcaccat ccgggctcca gacgggtcgg cggtcgtcgg
     5941 cggagtcgtt catcagttga cgttggttcc ggccgtcggc atccatcacc cagatgtcat
     6001 tgctgcccga ccgattggag aagaaagcga gttttgttcc atcgggactc cacgccggcc
     6061 tcaaatcatt ggccggatcg ttggtcagcc gtatgccagg taattgagcc tgggcgacga
     6121 caacgccagc gacggacaag accaagacgg tggccaggac gagaccaacg cttgaaatcc
     6181 tgtttcgtgt catgtggtga ttgcccatgt gccgttgtcc catgctctac aggttgatcg
     6241 cccaatccat ctcgctctgc tgttcccagt gctcacgggt ggcaggtggc gcatccaggc
     6301 acagccggtc ggattgaatg atcccttgca ggatacggat attcacgggg cacgcttcga
     6361 cgcaggccgg cccttccggt cgccagtagc acaggtcgca cttggtgtag acgttcttgg
     6421 atggatcgta ggtgatgcgc gtccgctgcc ccagcacctc gcggccagtg gcctcggtct
     6481 ccgagaatgt cgggaagggg caggcctcgg cgcagcggcc gcaggcgatg cacttggttt
     6541 cgtcgattat gcgggcgccg ctggtcggct ccacgggcag cgcgttggcc ggacagacgt
     6601 gatggcacgg cgcgtccgga cacatgagac atgggctttg atgatactgc cctcgatcgc
     6661 cgtaggccgt ggtgatgact ggatcgaccg aggtgttctc gtcgtagaag atgcggatgc
     6721 gagggatatc cgacaggcct gcctcgcggt gtgcgcgtga acaggccact tcgcacgtca
     6781 ggcaaccgat acacagtgtg gggtcgggca tgatcacgcc cgaggacaac tgcagttcgg
     6841 ccggacccgg atcgttcagc aggttgactc ccccgactcc gatcgcggcc acggccacga
     6901 acccggctcc agcttgcttg aggaaatcgc ggcgcgagac gacaagcggc gcagcgcctt
     6961 gagaggtgtt ctcattcggt cttatctctt cttgggccat ttgctcaatt ccctggtgag
     7021 cctgcagccg aaatcgcgtc agtttcgacg cccatagccc ctaggcaatg gagcggcaga
     7081 ggttggtatc atggacaata aggtgtggca tcccgcccgg ttcggaagcc ggatgcacgt
     7141 tcaacgccta cggaccatca ccggtaccgc agaccggatc gctccaatgt caacgtcggt
     7201 tcgcggtggc gtacctcacc ggccacgact ttcccgacgt agatgacatg atcgccaccc
     7261 gggtccagag tatcacttac ttcgcactcg aggtaggcca gcgcttcatc caacacggga
     7321 acgccggtga tgccggagtg ccaggacacg ctcgcaaatt tatgcggctg gatatggtga
     7381 ggcgtgccca gccgcgcagc caacggcgtc tgagcctggc ccagaaaatt gacggcgaag
     7441 acacgctgct cttccaatag tccctgggaa tagctcgtct tatccaccgc gagagccagc
     7501 agcaggggtt cgaaagacac ctgcatcagc caggaagcgg tgaaagcgcc tggctgtttg
     7561 gactcacctc cggtggtgat gacgtatact ccgtaaggga tggccagcaa tccgtcctga
     7621 acgttcatgg ctgatcacat gcctcaaaag atgattgacg ccgtcgccgc agccttcagt
     7681 acagcttctc gtttccgcga taacggacga cgacgcggcg cgacgcatcg gcctgatcat
     7741 ccccctcttc gacccggagg gcgtagagcg cctgccccga gttcaattgc tgcaatagct
     7801 cgtcgaaggc gggctgatga acttgcgcca aatccagaag catcacctgc gcttccggca
     7861 aaatctgccg ggctgactca accaactcgt ccgcgacaaa cagtacatcc acatcgggcg
     7921 ggagctgact gagcggacgc atcacgacct cgatcctgaa tccggcccgt tcgagtttcc
     7981 gccgcaggat cgaatagctg cggatgagag ccggcggtaa ggcattcagg tcataggcca
     8041 cagccgcctt cttgaccaat cgcggggtgg gcacctgatc gtccatgaag ccctggtgcg
     8101 ttcaatgctg gctaagacac tgtcaagaga cagccctttc ccggttgcga acggcacgga
     8161 ctcaatcgtc ctcaaccacc ataacccagg cattcatcgc gcccggatga taatcgcaat
     8221 ggtaggcgaa gacaccgggt ttacgaaacg taaatttgaa ggcgcgggtt ccaacgccgc
     8281 ccgtcgcgcc gctaaaaacc ggcggggaat ccttcgacac gatcgtatgg tagaggactc
     8341 cgacgggttc ccaggtgacc tccgttccaa cggtgacggt caccacttgc gggtagaaat
     8401 agtaatcgcc gacggtaatc tccatccggc cttcggccgg tgtcgcagtt gctgcccgtt
     8461 gtgtcgcgag aggggatggc gctgcttttg cctcacgagt cacccccaga gtcgatgccg
     8521 gtgtcggcag ggtcggccgg atgagtgcag tgggcgtcaa agccgacggt gcgatctcgg
     8581 ccggcgggct tggcgccgaa ggtgtcgccg ccccgcccag acaggcactg gttgtccacg
     8641 cagtggatat caacgtgatc agaagtttga cgacgaatct tgctctccag tgtggccatt
     8701 tcgacgaggt tccagcctgc acgatgtgga gcttagccgg cttcaagaca gtcctcacac
     8761 agcgcgctgc agctgttcgt cgacgcgata caggcccaga atcgcgccgg ccgtcctgtt
     8821 cagccagcca tgcacgacgt tgttcgaggc aatatagcct ccttgagcat gttgccaggc
     8881 cgcgccctca atactcgtaa atatcccacc tgcttccacg aggatgacag cgccggcggc
     8941 gacgtcccag agtttcagcg cgtcgaggcc ataataggcg tgcaggcgac cggccgcgac
     9001 gtagcacaag ccgagcgcgg gggagccgag tgtgatgagt tgaaacgtct cgcccgccac
     9061 atagcgcgcc agcttgaggg ctttgcgaag ttcgtcgtca ttcccgcgcc agtccgtgcc
     9121 caccactgcc tgttcccagg cttcgcggcc atcactgaag agatccacat gtatcgggtg
     9181 gccgttcaag aacgcgccgc tgccggtcac cgcatggaac agctcgtccc gacacggatc
     9241 gtagaccacg cccacgcgat aaaggccggc gacgcgatat gcgatgctga ccgcaaagat
     9301 ggggatgccg tgcagaaagt tgagactgcc atccaccgga tcaacgatcc acaatggatc
     9361 cgcctgcgtg tcctgcacgg catcgctttc ttcgagcagg aagtgggcct ccgggaactc
     9421 ctgactaatg acgtccacga tgcgcttctg gacatcgatg acggcgccgg cgacgacatc
     9481 gcgcggcccc ttccgctttt catagcccgg cttgccgagt cgagtgacgg ccagttggcc
     9541 gcccgttcgg gcagccagga tggccgtctc cagcacgcgg ctcgtgtcgt tattcattga
     9601 cgaccaccgg atgtcccatc tgtcgaagaa gggccatgat ttcttcaaca gttatgcgcg
     9661 tcggatcgta ggagacgtta agcacggttt cgccgccagt cacgctcaca atagcatcgt
     9721 tctccatgag gtcaacggcg atgacagcga cgtcgtccgc gtcggacggc ggaactgaac
     9781 ccggcgccca attgaaggca acagtttcaa ccggcgtcgc agccgtaggc tccgccgtcg
     9841 gcggtggttc ggttggcacc tcggttggcg cctccgtagg cggcgctgcc tgtgtgctcg
     9901 ctggcgtgct cgacgcctga gtcggcggcg cctcggtagg cgcctcggtg gcggcgcttg
     9961 ggctgccgca gcccgctacc atcatcacga cactcacaac cagaccaata cgtaaagcaa
    10021 ctctcattgt taattctctc ccgtcacagg gtgaaggtta cccacctatt ttgcctaatt
    10081 catttctgaa agtcaaatct tcgaggtcaa cggcacattt caattctgga gtcgatacca
    10141 tctcacggcg gtgaaagatc atcggcagga atcgaaaacg tccgcgactg tccgccgtgg
    10201 cggatgacga ccaggacttc agtcagcacc agatcggtcg ggccgactct cccctcggca
    10261 gtgtacaaac ccgctccctg gctcgcgagc gtcaccgaaa agtcttcgtc gtgttcaccc
    10321 tccatccccc ccatcccgcc gaccatcgtc aactcaactg tggcatccac aaccggctgt
    10381 ccagccgcgt cgatcagagc agcctggtaa gtcgtcagga cgcccagccg tgggggataa
    10441 ggctcaaaac acaggctcag agtaagatcg ctctgagtct gtgtccactg ctgggtggcc
    10501 tcacacttca gcgtggctgg aatcatggac gaggccggtg aagccgtgac gctcgagctg
    10561 gcaggcacgg tcggcgacgg aacaggtctg gtcagtgtcg gttctgccgt caagctgggc
    10621 gctgcggtcg tcgagcttgc acctgtcgtc ggtgtggcgg tctccaccgc aaccgacggg
    10681 cgacaaccga cgatgcagac gctgaccagc aacaaaccaa caatccacct gactgtttgc
    10741 accagatggt cattcatgcc ttagcacctg ggctaccaga cagacagagc cagccatctc
    10801 gcggatgatg cattaaagcg atgtgacgac ctgtcgtccc atgcaatgca ttgctctctg
    10861 atacagccag ctctgtcttt tcaggacact ggtcgctcga ctctgcctgg gcttgaaccc
    10921 tccttacttc atcaaggtga tcaggccacg aggcttaaca ccgcgcgctc ggtcaggccg
    10981 atcgcgaggt ccaccttgta gccgttgttg cttaagggcc gggcttcacc acggatgagc
    11041 gccgcggcgc tcttgatggc cgacttgaca tccttgccca cgagcgcctg ctcgactacc
    11101 accgctcgcc acggcacggg cgctacgccc cccagcacga tcctcccgtc ctgccagacc
    11161 ccgccatcga gggtcgctac cacggcgacg ctcgttaggg cgaagtcata cacctgccga
    11221 tccttgagtt tgatccaggc catcttggtg ttggccgctg gcggcggtac tttcacgtcc
    11281 accatcagct cgttcgcctg caggacgttc tccctcagca cgtcctcgcg cggaccgatg
    11341 aagtagtcat cgaaggctac ttcccggcgg cccgatggac tggcgatcgt cgttctggcc
    11401 tttagcgcca gcaaggccgt ggccgtatcg gacgggtgca cgatgtagca cagttcgccg
    11461 ccgatgatgg cgtgaggctc attgtcgccc gtcacggcga aacagaagtc gccgcccttc
    11521 ttgtagcacg ggaagctctc gccgcgcagg aaccagcagc gcgggcgctg gttgatgttc
    11581 ccgcccaacg tgccgaagtt gcggatgagc ggcgaggcga cgctgaacgc ggcatctgaa
    11641 agaaccgacc agccagtccg gatctcttca gattcgacga cgtccgtgag cgtcgtcgcg
    11701 gcgccgatct tcaaatcggc gccttccttg gcgatgccat gcagctcggg gatggtggtg
    11761 atgtcaatca acacctcggg atagggcatc cccggcccct ggacccagtc cttcatcacg
    11821 ccggtgacca gatcgctgcc gcccgccacg accttcgact tggcgccgta tttctccaga
    11881 agcccgacgg cttcctctac ggtcgtcgca tcatagagtt caaaagcttt cattgctcag
    11941 tcctcctcat ccctgatccg gctgcctagg cagcccgctt cagggcttcc aagaccttgc
    12001 gacgggagat cggcatgtct tcgatccaca caccaatcgc gttataaacc gcgttggcga
    12061 tggtcggggg tggaagagcg atcggcggct caccggtgcc ctgggcccca aacacccccc
    12121 actccttggg cttttccacc aggatcacgt caatcgtgcg cggcacatcc ttaatgctca
    12181 ggaccttgta gtccagaagg ttgccattca acgggaggcc cgtcgcgaag tccaccttca
    12241 gctcctcggt cagcgccgcg ccgatgccca tgattacacc gccttcgacc tgttgctcgg
    12301 tgcccatgcg attgatggcg cgtcccacgt cgtgggcggc cacgtacttc agaacggtga
    12361 ccgtgccggt gaccgtatcc acttccacct cggccgcctg agccgccatg tggatgcgct
    12421 cccatgttcc tggatggaaa tgggcaccgc gtccaatgac ggcggtgttc gggacgaccg
    12481 catccaccgc atccccgatc tccatgctga cttccggatt gtctttgaag acgaccagtc
    12541 catcccggat gtcgagttgg tctggagtaa cctcgatctc tcgcggcgga tcctccgctg
    12601 cagcggcgtc cacgaaggcc tgagcgcccc cggccaggat ctgattttta gcgtcaatcg
    12661 cggcctcgta caccccccag ccaccgctgt tgatctgccg gctcccggcg gtaacgccgg
    12721 tgtcaacggt catggcagaa tccacgccga cggatatgtt aaccgctgac agtggaaccc
    12781 caagcgcctc ggcggcgatc atggccatag ccgtccgctg cccggagcct acctcggtcg
    12841 ccgcagagat cacgtccaca cgaccattgt tgtagacggc gatggtcgcg gtggagggat
    12901 ggccgccggc gccgtggctg caggtgtggc aggcgatgcc gatgccgtgg aaaacgcccg
    12961 gccgcacttc gttcgccccc ggcgcgtgcc acttctccgc ccagccgatt cgttcggccg
    13021 cctgctccag ggcgtcgcgc aggcccggat tgctgtacgg cgtgccatcg tccagattgc
    13081 caagctcgtt gatgttttgc agccggatgt ccagcggatt catgccgatc gcatacgccg
    13141 ccttgtccac cgcgatctcc tgcgccaggg tggcggtggg atgggagacg caccgcaacg
    13201 ttccgttgcg gaaggaatta gtcatgacgt ccgtcccgtc caattccagg ttggggaagg
    13261 cgtaggactg ctcgaagccg acccagccgc cggttacctt cccaccgcgg acggcgccga
    13321 cgttgccgag cgtggaaaag tgcgcggcca ccatcttgcc atcgcggttg acgccgatgc
    13381 gcgaagtggt ttcctgttcc ccacgcccgg tgcggtagat gaaatcctcg taccgggtgg
    13441 cgatgtagcg gatcggccgg ttagccaccc tggcgaggat gctggcgatg ccctcctcct
    13501 ggtcaacgct gcgcagattc ccgtagctgg cgcccatata gccctgctgg atcacgcgca
    13561 ccttgttcgc gggaattcca agcaactgcg ccagcgaatt gcggtcgccg tgcgggtggc
    13621 gggtggtgcg atagtggatc aactggtcgt tgtcccagta agtgaggatg ttgctcagct
    13681 ccagggccgc gtgctgatgg aatgggctgc gactaacgct ctctaccacc acttccgcct
    13741 ggtccagtgc ggtcttgccc tcaccccgca ggaaagggtt ggggatgccg atgatcgtgc
    13801 cgtcgaactc gttttcccac tgcttggacg tcgagggcag cgccgcctcc cgaaagttga
    13861 gcacggccgg cagcacctcg tactcaactt cgatcaggcg aatggcctcg tcggcgatgt
    13921 gctcctcgat agccgccacg gccaccaccg gcgcaccgac ctgaaagacc tcttcggcga
    13981 agagataacg actgggattg gcgccgccga cccggctttc caaatactct tcgggcaggt
    14041 tgaaccggtg cagcacggcc accacccccg gcatcgcctc cgccttgctg gtgtcgatgg
    14101 acaggatctt ggcgtgcggg tgggggctgc gcaagagctt catgtacgcc atgttcggca
    14161 gcggcggcgt gttctccagg tagacgccag tgtggctgac gacgccctgg ccgtgcacac
    14221 gcttttgcct ggtacctacc accgagaaac tcgggctttc ttcctgccac gggggtacct
    14281 tggccttacg gctgacccct ttgaggccga gtctggcatt cagagcagtc atgagttcag
    14341 gctcatcggc taccaggtct agccacacag ggtcgcgcgc cagttcacgc aactgagccg
    14401 gagacaggct ctgcgcatag gccgggtccc ccaaggcgcg gataggatcg atgcgtgctt
    14461 taacactcat ggtgactcct ccttgaccta actggccgct cgcattaact cggccgcttt
    14521 gaagaccgaa ttaaagatca tggggtactc ggagcagcgg cagatgttgc ccgccagcgc
    14581 ctcccggacg tccgtttcgg tcgggttcaa gttcttggag agcagggcat aggtggacat
    14641 gatgaagccc cgggtgcaaa tgccgcactg ccagccgcct tcctcccaga acgccttctg
    14701 aatcggatgt agatccgctt cccccacgcc cgtcgcgaga ccggccactg tcacgatctg
    14761 ttgaccccgt cccaggcgag cggatagaat cgtgcaacca ttcaccgcgc gcccatcgac
    14821 gactaccgcg caggcgccac actgcgcccg gtcgcagccc aggttgatgg cagtccccag
    14881 gccaagtttc ttggtcatgg tctcccacag gctctcgcgc acgtctacgg tcacttcgtg
    14941 ggcccgcccg tctatgttca gggtgacggc gcgcatggtg gccggaagcg tcgtgcccac
    15001 ggtgaccgtc tcgccaggca cctgcacttc cttgatgact tccaccgtct ccggctgccg
    15061 cagcgcgatg acagccgtgg ctgccgcgcc ggtcaccacc acgcctgcgc cggcccccac
    15121 caggaaatct cgccgtgaaa tcttcagcgg cgcactttct ggttcctcaa caggaacctt
    15181 ctcttcgtct gccatgagaa tacctcccca tgtgctggtg atgctggccg aacgagcctc
    15241 ggcccagcgt cttgagcttg atgcttaatg aaatgtagtg gctctcctct cctttcccct
    15301 ccatgggcca tgctaaactt gttccgtgct aaccgcctcg gtcgatacag gatcgctggc
    15361 ccgatcctca tataaggtag caagggcctg cttgcactcg ttgagcgacc gccataattc
    15421 cgccagttcg tcggcatgca gcttctgata ggcttgcagt tgctgctcaa atctcaggag
    15481 gcgactcgcg agatctacag gcgggttcac aatagacctc cccctgcacc ggcgatgagg
    15541 aacacagccc tgtcaggtta gacccaagtt agagagatat tagagagata taacctttat
    15601 atcccagaac agggggaaac agaagaggcg ggagggtcaa taactggccc aaaagggcca
    15661 atctgatctt acagcggact ccaattgcag gtacactatg ccggactgga ctttgtccat
    15721 tttcatattg acgccacata taggcattca ttacgacatt gtgcccccat atctggggcg
    15781 acatgtcgca gaaaccgcct aaaacgggcc gaaaaggcca aattggacaa ag
//
LOCUS       DBNHXB010000327         6743 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2270113, whole genome shotgun sequence.
ACCESSION   DBNHXB010000327 DBNHXB010000000
VERSION     DBNHXB010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2270113"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..331)
                     /locus_tag="AC162G_13260"
     CDS             complement(59..331)
                     /locus_tag="AC162G_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7990 family protein"
                     /protein_id="HIR5076820.1"
                     /translation="MTEHTAPKKSLWENVKEVLYGMGRHDMTRYAIRTRASMEHLFIL
                     ITMGDLLGIPILPPYYSLRLLPYVVPQIATWKRRMLREKDISDAMF"
     gene            complement(422..721)
                     /locus_tag="AC162G_13265"
     CDS             complement(422..721)
                     /locus_tag="AC162G_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7990 family protein"
                     /protein_id="HIR5076821.1"
                     /translation="MSSSSASSSHNPGWRAKIRGFLFGMMGMEFTRHALEMRASTETL
                     FMLATVGDMIGVPILPPYYSLRLLPYVVPQIATWKRRVLREKEFTDEHDYDLHGL"
     gene            867..1517
                     /locus_tag="AC162G_13270"
     CDS             867..1517
                     /locus_tag="AC162G_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076822.1"
                     /translation="MDQLSQTILVVLIAIILGWFAFGVVWNIRRGNAVLRWMQGGLPR
                     LGEKTTLRWLGSSAVALGIAKARSPFRQVDLLLVMEPRDVPWFWLLAHARGRRDMLIV
                     RGLLTSAPRYEFDLLAPGSWSEREANRGDTRRWESEPLGGANFHAPAPTRPLSRQLAP
                     DALEAARKVQPNVWRLSSRRESPQLELHVPLPNPKHTNAAQFFDALRALAEQMGSR"
     gene            complement(1657..3576)
                     /locus_tag="AC162G_13275"
     CDS             complement(1657..3576)
                     /locus_tag="AC162G_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR5076823.1"
                     /translation="MNTLFIVVIGAVVIYLAYNFYARRIDRNVIQASASRATPAKLYM
                     DGVDFMPASRNVLYGYHFKSIAAAGPIVGVITAANLWGWLPALLWLVIGVSFIGWASD
                     YSAIMLSVRNDGNSLSAIAHKLIAPRTRTIMFVFIFFYLLLVSGAFLGIMTALMAARP
                     DVPLGILTLAVMGLLASYLLYQRKMGLTVVTVIVVGVTLAAMLLGPVGAAPRLDDQGR
                     QVGIDWVGPINSTVVTVNKAIDGLTGGNAIMTVTDPTNQDPRITNPALYNADTGEIKT
                     LPSYLFWALFLLVFSYLGANLPIWRFAQPVNYIGFWITLITIVLSAVGAVLAPLIKPE
                     VGAFALPAFCSSADVANKLCQAADGGFTPLATGAIQPIWPMLFVTIACGAISGWHALF
                     GSVGTARQLEYETDGLPVGGGAMFSENTLALLSLVAVSIAGTGGGPGRFAAGVGSLMS
                     VLFGDGFRFFGTALGFGAFMVIVLTVVQLLFRVMRVTLTEWAGEAIPAVKNMHVSTIV
                     SMVLTVALVLTGTWVYLWQLFGASNQLMAALSLLVVTVWLKSEKRNPAYALWPMLFMY
                     ITTIAATIVTAYNLYATIVLKQSGLPVIGAWAMIVIAVLLIVAALFIGYDGWKAYQRY
                     ARGERPTPAPASAGK"
     gene            complement(3700..4086)
                     /locus_tag="AC162G_13280"
     CDS             complement(3700..4086)
                     /locus_tag="AC162G_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076824.1"
                     /translation="MEDKPLRVEIVAYAPTAFYHCTHCEVIWQETGFSKGLHEEQVRS
                     ALPPDLQQDYQAVSDWVRRLFRIHCDRVIVKVVDAASLEGFWKSLRHGLGRYPAVIVE
                     GRAKFSGTDFAAAEAEIADRLNAVPA"
     gene            complement(4189..5133)
                     /locus_tag="AC162G_13285"
     CDS             complement(4189..5133)
                     /locus_tag="AC162G_13285"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005091.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HIR5076825.1"
                     /translation="MRIAIVGTGGIGGYYGGLLARAGHDVVFIARGEHLRAIREQGLK
                     IHSVHGDFEVYPAKATDNPEEIGAVDLTIFAVKTYDIDSAAQLMRPLVGPQTVILPLQ
                     NGVESAAALSRHFDRQAILGGATWIVASIVEPGVIRQESQFRRIALGELDGRLTERVK
                     AIGDALAEAGATVEVSTEIEKVLWTKLLFIASFSGITSVTRAPAGPVMACVESRLLLE
                     RAMREVEAVARARGIRLDADVVEKTMTFCANLDPAATSSMQRDVAAGRRLEHDAVNGV
                     VVRLGREAGVGAPVHEFIWTCLKVVDSMVGGRWQMADS"
     gene            complement(5258..>6743)
                     /locus_tag="AC162G_13290"
     CDS             complement(5258..>6743)
                     /locus_tag="AC162G_13290"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HIR5076826.1"
                     /translation="WLPVKPGTNVPVFTAMAHVIIREGLVNKKFVEERTEGYEEFVQS
                     VEKFTPEYAEAISGVDRNLIVEAARMYATAKNGAIYWGMGISQLSHGTASALGLIHLA
                     LLTGHVGREGTGLNPLRGQNNVQGASDSGAMPFHYPGYMEVDDDENAARWEKAWNVEP
                     GGLSRKRGLTTTEILSNAKPGGVRALYIMGENPMMTEPNLNVTRHHMEQLEFLVAQDV
                     FINESGAFADVILPAASWAEKEGTFTNTDRRVQRVRQAIEPRGQSRPDWQIICDLANR
                     IEKRLGRPNTARWSYEEPEEVFREMAGVATMLKGVTYERIENVGLQYPVPDETHPGTP
                     FLFADTFPSGRGRFFPLEYVPVTELPDDEYPFILTTGRLLEHWHGGTMTRHSQLDALF
                     PEALVDMHEIDAARCGVKTGDVVRVSSRRGSVVLRVNVSPKISPGVVFIPMHFAEAAA
                     NVLTIDALDPQAKIPEFKACAVSIGIAREEELLNPDAWQVRGRY"
BASE COUNT         1309 a         2225 c         1981 g         1228 t
ORIGIN      
        1 gagttagagt gaggagttag agtattgcac tctaactcta actccatact ctaactcact
       61 agaacatcgc gtcggatata tccttctcgc gcagcatgcg ccgcttccag gtggcgatct
      121 gcggcaccac gtaaggcagc aggcgcaggc tgtaatacgg cggcaggatg gggatgccca
      181 gcaggtcacc catcgtgatc aggatgaaga ggtgctccat gctggcgcgc gtgcgaatgg
      241 catagcgggt catatcgtgt cgacccatgc cgtacagcac ttccttcacg ttttcccaaa
      301 gactcttctt gggcgcagtg tgctcggtca tcgccgctcc tcagaagtga aggatgaagg
      361 ataagggatg aaggatgaag aaatgtcgtc tcagccttca gatccctgtt ctcaccccct
      421 attataatcc atgtaagtca taatcgtgct cgtcggtgaa ttctttctcg cgcagcacgc
      481 gccgcttcca ggtggcgatc tgcggcacga cgtagggcag caggcgcagg ctgtaatacg
      541 gcggcaagat cggcacgccg atcatatcgc cgaccgtcgc cagcatgaac agcgtctccg
      601 tgctggcgcg catctccagc gcatggcgcg tgaattccat gcccatcatg ccgaacagga
      661 acccgcgtat cttcgcacgc cagccggggt tatgagaaga ggaagcagat gatgaggaca
      721 ttggcgctcc ccgcgctccg gctttataat cgacgcgctt ctcacagccc gtcgtcagca
      781 gtgacgatga acgtgaagca cgatccgcga cgcgatgggc gacaagcgat aggcgataag
      841 cgataagcca tccgcgataa gcgaaaatgg atcaactctc tcagacaatc ctggtcgtgc
      901 tgatagccat cattttgggt tggttcgcct tcggcgtggt ctggaacatt cgccggggta
      961 atgccgtgct gcgctggatg cagggcggcc tgccccgcct cggcgagaag accacgctgc
     1021 gctggctcgg ctcgtcggcg gtcgcgctgg gcattgccaa agccaggtcg ccgtttcgcc
     1081 aggtcgacct gctgctggtc atggagccgc gcgacgtgcc gtggttttgg ctgctggcgc
     1141 acgcgcgcgg gcgacgcgac atgctgatcg tgcgcggcct gctgacgtcc gccccgcgtt
     1201 acgagttcga cctgctcgcg ccgggcagct ggagcgagcg cgaagcgaat cgaggcgata
     1261 cccggcggtg ggagagtgag ccgctcggcg gcgcgaattt ccacgcgccc gcgccgactc
     1321 gaccgctctc gcgccagctc gcgcccgatg cgctggaggc cgcccggaaa gtccagccga
     1381 atgtgtggcg actttcctca cggcgcgaat ccccccagct cgagctgcac gtgcccctgc
     1441 ccaaccctaa acataccaac gcggcgcagt tcttcgacgc gctgcgcgcg ctggccgagc
     1501 agatgggcag ccgctgaacc tcacccccac cccctccctc cccctctcct tgcaggagaa
     1561 aaagggggac cagggatgca gtgaggtcgg acgctacacg ataagcctcg ccccgacgac
     1621 tcttcggggc gaggcttgtc tattcagttg tcaacctcac ttgcccgcag aggcaggcgc
     1681 aggcgtcggc ctctcgcctc tcgcgtagcg ctgatacgcc ttccagccgt cgtagccgat
     1741 gaacagcgcg gcgacgatca gcaacacggc gatgacgatc atcgcccacg cgccgatcac
     1801 aggcagaccg ctctgcttca ggacgatggt ggcatacagg ttgtacgccg tgacgatggt
     1861 ggccgcgatc gtcgtgatgt acatgaacag catcggccac agcgcgtaag ccggattgcg
     1921 cttctccgac ttcagccaca ccgtcacgac cagcaggctg agcgcggcca tcaactggtt
     1981 cgacgcgccg aacaactgcc acagataaac ccacgtgccg gtgagcacca gtgccacggt
     2041 cagcaccatc gagacgatgg tggagacgtg catgttcttc accgctggga tcgcctcacc
     2101 ggcccactcc gtcaacgtca cgcgcatcac acggaagagc aactgcacga cggtgagcac
     2161 gatcaccatg aacgcgccga agccgagcgc cgtaccgaag aatctgaacc cgtcgccaaa
     2221 gagcacagac atcagactac cgacgccggc agcgaatcgg cccgggcctc cgccggtccc
     2281 agcgatagac accgccacca gcgaaagcaa cgccagggta ttctcggaga acatggcacc
     2341 gccgcccacc ggcagcccgt cggtctcgta ctccaactgg cgggccgtgc cgaccgagcc
     2401 gaacagcgcg tgccagccgg agatcgcgcc gcaggcgatg gtgacgaaga gcatcggcca
     2461 gatcggctgt atcgccccgg tggccaaagg cgtgaatccg ccatcggccg cctggcagag
     2521 cttgttcgca acgtcggctg aagagcagaa ggcgggcaac gcgaaagcgc cgacctccgg
     2581 tttgatcagt ggcgccagca ccgctcccac cgcgctgagc acgatggtga tgagcgttat
     2641 ccagaagccg atgtagttca ccggctgggc gaagcgccag atcggcaggt tggcccccag
     2701 gtaggagaag accaacagaa acagcgccca gaagaggtaa gacggcagcg tcttaatctc
     2761 gccggtgtca gcgttgtaca gggccggatt cgtgatgcgt gggtcttgat tggtcggatc
     2821 ggtcacagtc atgatcgcgt tgccgccggt caggccgtca atcgccttat tgacagttac
     2881 gacggtgcta ttgatcgggc ctacccagtc gattcccacc tgcctgccct ggtcatccag
     2941 tctcggtgct gcgccgacag gaccaaggag catcgccgcg agcgtcacgc ccacgacaat
     3001 cacggtgacg accgtcaggc ccatcttccg ctggtacagg agataactgg ccagcagtcc
     3061 catcacggca agtgtcagga tcccgagagg tacatcaggg cgcgctgcca tgagggccgt
     3121 catgatgccc aggaacgccc cgctgacgag cagcaggtag aagaagatga acacgaacat
     3181 gatcgtgcgc gtgcgcggcg cgatcagttt gtgggcgatg gcgctcaggc tgttgccgtc
     3241 gttgcgcacc gacagcatga tcgccgagta gtcgctggcc cagccgatga acgacactcc
     3301 gatcaccagc cacagcagcg ccggcagcca gccccatagg ttcgccgccg tgatcacgcc
     3361 gacgatcggt cctgcggcgg caatggactt gaagtgatag ccgtacagca cgttgcggct
     3421 ggccggcatg aagtccacgc cgtccatgta cagcttggct ggcgtcgcac ggctggcgct
     3481 ggcttgaatg acgttgcggt caatgcgcct ggcgtaaaag ttgtaggcca ggtagatgac
     3541 gaccgctccg atgacgacga taaacagggt gttcatacgt gttcctcctc aaccagaatg
     3601 atcgatgagc ggataatcca cacggttgcg aggcaaacga ccacggtctc ccccgtgcat
     3661 cacctccttt cccacagcag tcgatgggcg ccgtcccaac tacgccggca ccgcgttcaa
     3721 tcggtcggcg atttcggctt cagcggcggc gaaatccgtg ccgctgaact tcgcccgccc
     3781 ttcgacgatg acggccggat aacgccctag gccgtgccga agcgacttcc agaatccttc
     3841 caacgaagcc gcgtcgacga ctttgacgat cacccgatcg cagtgaatgc ggaacaagcg
     3901 ccgaacccag tccgacacgg cttgatagtc ttgctgcaga tcgggcggca gcgcgctcct
     3961 gacctgctcc tcgtgcaggc ccttgctgaa gccggtctct tgccagatca cctcgcagtg
     4021 cgtgcagtga taaaaggccg tcggcgcata cgctacgatt tccacccgta aaggcttgtc
     4081 ttccatgttc ccctccccgc ttatcgccta tcgcctgtcg cgtatcgcct atcgcgcatc
     4141 gcacatgcaa tcgaggttga ccggcgataa gccatatgcg atatgccatc agctatcagc
     4201 catctgccat ctgccaccga ccatcgaatc gaccactttc aagcacgtcc agatgaactc
     4261 gtgcaccggc gcccccacgc cggcctcgcg cccgaggcgg acgactacac cgttgaccgc
     4321 gtcgtgctcc agacgccgcc ccgctgccac atcacgctgc atcgaggatg tcgccgccgg
     4381 atcgaggttc gcgcagaagg tcatggtttt ctcgaccaca tcggcgtcga gccggatgcc
     4441 gcgggcgcgc gctaccgcct ctacctcgcg catcgccctt tccaaaagca aacgtgactc
     4501 aacgcatgcc atcaccggcc ctgccggcgc gcgagtcacg ctggtgattc cgctgaacga
     4561 ggcgataaac aacagcttcg tccatagaac cttctcgatt tccgtgctga cctcgaccgt
     4621 cgcgccggct tctgccaggg cgtctccgat cgccttcacc cgctccgtca gacgaccatc
     4681 cagttcgccc agcgcgatgc gccggaactg gctttcctga cgaatgacgc ccggctcgac
     4741 aatcgacgcc acaatccacg tcgcaccgcc gaggatggcc tgtctgtcga aatggcggct
     4801 gagcgcggcc gcgctttcga ctccgttttg caaaggcaag atgacagttt ggggaccgac
     4861 cagcgggcgc atcaactgcg ccgcactatc tatatcatac gtcttaaccg cgaatattgt
     4921 caagtctacc gcgccaattt cctccggatt gtcggtggct ttcgctgggt agacctcaaa
     4981 atccccatgg acgctatgga ttttcagtcc ctgctcacga attgcccgca aatgctcgcc
     5041 gcgcgcaata aagaccacat catgcccggc acgtgccagc aaccctccgt agtagccgcc
     5101 gatgccccca gtccccacaa ttgcgatgcg catcacttgt aataaccctg ccgccgccgc
     5161 agggttacgt caagccattc tccaaccagg gattatgttt tgctgcgaac tcaacttgcg
     5221 acgcacttgg caggtgcgtc gcaagttcaa gtctgtccta atagcggcca cgcacctgcc
     5281 aggcgtcggg attcagcagc tcctcctcgc gcgcgatgcc gatgctcaca gcgcaagctt
     5341 tgaactcagg gatcttggcc tgcggatcga gcgcgtcgat cgtgagcaca ttcgccgctg
     5401 cctcggcgaa gtgcatggga atgaagacca cacccgggct gatctttggg ctgacgttga
     5461 ctcgcaacac gaccgagcct cgccgcgagg acacgcgcac cacgtcgccg gtcttcaccc
     5521 cgcagcgagc ggcgtcgatc tcgtgcatat ccaccagtgc ctccgggaac aatgcgtcca
     5581 actgcgaatg gcgcgtcatc gtcccgccgt gccagtgctc cagcaagcga ccggtggtga
     5641 ggatgaaggg gtactcgtcg tcaggcagtt cggtgacagg cacgtactct aatgggaaga
     5701 aacgaccccg cccggacgga aaagtatctg caaacaggaa cggcgtgccg gggtgcgtct
     5761 cgtcaggcac gggatattgc aacccgacgt tctcgatgcg ctcgtacgtc acgcccttca
     5821 gcatcgtcgc cacgccggcc atctcgcgga acacttcttc cggctcttcg tagctccagc
     5881 gcgccgtgtt cggccggccc agtcgcttct cgatgcggtt cgcgagatcg cagatgatct
     5941 gccagtcggg ccgggactgc ccgcgcggct cgatcgcctg gcgcacgcgc tgcacgcgcc
     6001 ggtcggtatt ggtgaacgtg ccttcctttt cagcccacga agccgctggc aggattacgt
     6061 cggcgaacgc gcctgactcg ttgatgaaca catcctgcgc gacgagaaac tccagttgct
     6121 ccatatggtg acgagtgacg ttcaggttcg gctcggtcat catcgggttc tcgcccataa
     6181 tgtacagcgc gcgcacgccg cccggcttcg cgttcgagag aatctcggtc gtcgtcagcc
     6241 cgcgcttccg gctcaagccg cccggctcga cgttccacgc cttctcccac cgggctgcgt
     6301 tctcgtcgtc atcgacttcc atgtagccgg gatagtggaa tggcatcgcg cccgaatccg
     6361 acgcgccctg cacgttgttc tggccgcgca gcggattgag gccggtgccc tcgcgcccca
     6421 cgtggccggt gagcagcgcc aggtggatca gcccaagcgc gctggcggtg ccgtgcgaga
     6481 gctgagagat gcccatgccc cagtagatcg cgccgttctt ggccgtggcg tacatgcgcg
     6541 ccgcttcgac gatgagattg cgatcgacac ccgagatggc ctcggcgtac tcgggcgtga
     6601 atttctcgac agactgcacg aactcttcgt agccttcggt gcgctcttcg acgaacttct
     6661 tattgaccag cccctccctg atgatgacgt gcgccatcgc ggtgaagacc ggcacgttcg
     6721 tccccggctt gaccggcagc cac
//
LOCUS       DBNHXB010000328         2925 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_424558, whole genome shotgun sequence.
ACCESSION   DBNHXB010000328 DBNHXB010000000
VERSION     DBNHXB010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_424558"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="AC162G_13295"
     CDS             <1..504
                     /locus_tag="AC162G_13295"
                     /inference="COORDINATES: protein motif:HMM:NF013781.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR5076827.1"
                     /translation="MGLCDTVDPLAGSYYVEWLTNEMERRIVAEMQRVDDEMGGIVHA
                     VSEGAVQREVAYQALKVEQGIQNGTIAKIGVNRYRLEGEQRGVQLHEYDPEQAEESIR
                     RTNEVRASRDAQAVATALAKVRDAASSGQNVMPAIMDAVRAYATLGEITQTLKQVFGV
                     FREPVRL"
     gene            551..1708
                     /gene="serC"
                     /locus_tag="AC162G_13300"
     CDS             551..1708
                     /gene="serC"
                     /locus_tag="AC162G_13300"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIR5076828.1"
                     /translation="MATGVMAQTGPALSKAEGLQAEAAGRVHNFNPGPAVLPLPVLER
                     ARDELLSYDGSGISVLEMSHRSPQFEGIMARAEADLRSLLNIPDSYTVLFLQGGASLQ
                     FSMAPLNLRGPDQSADYILTGSWAKAAIKEAEKAGRVRTAGSTESSNFDYIPSPAELD
                     LDPRAAYLHFTANETIHGVEWANEPEPPPGVPLVCDASSDIASRPIDVSRYGLIYAGA
                     QKNLGPAGVTLAVIRNDLLDRVPSRLPVMLDYRALAESKSLHNTPPCFSVYLVGLVLR
                     WLKDIGGLQAIARHNEAKARLVYQAIDSSGGFYRGHARLDSRSRMNVTFRLPSEDLEK
                     RFAKQAEAEGLVGLNGHRSVGGLRASLYNALSIDAAQSLVEFMAEFQRVNG"
     gene            1811..2758
                     /locus_tag="AC162G_13305"
     CDS             1811..2758
                     /locus_tag="AC162G_13305"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HIR5076829.1"
                     /translation="MTHKILVADGLSGDGIALLRTAGEVTVNPKITADELLAALPAYH
                     ALVVRSRTKVTAQVVEAGTNLKVIGRAGVGVDNIDVGAAIARGVTVVNSPLAASVAVA
                     EHTLGLMLALARNIPIADALMKQGKWEKNAFVGSELFGKTLGLLGIGRIGAEVAKRAE
                     AFGMTVLAYDPYLTAEEIRARRAVPAAFDEVIAKSDYLSLHLPLTEETRGLIGLEQFA
                     MMKKGARLICAARGGVIDEDALRVALDSGALAGAALDVFASEPPLPESIAAHPKVIAT
                     PHVGAQTTEAQASAGLMIAEEVLAVLQGQGPRWRVTTNE"
BASE COUNT          574 a          823 c          999 g          529 t
ORIGIN      
        1 atgggcctgt gcgacacggt cgatccattg gccggctcgt actacgtcga atggctgacg
       61 aacgagatgg agaggcgcat cgtcgccgag atgcagcgcg tcgacgacga gatgggcggc
      121 atcgtgcacg cggtgagcga gggcgccgtg cagcgcgagg tagcgtatca ggcgctgaag
      181 gtcgagcaag gcattcagaa cgggacgatt gcgaagatcg gcgtgaatcg ctaccggctg
      241 gagggcgagc agcgcggcgt gcagttgcac gaatacgatc cggagcaggc ggaggaatcg
      301 atccggcgga cgaacgaggt gcgcgccagc cgcgatgcgc aggcagtggc gactgcgctg
      361 gcgaaagtac gtgacgcggc gtcgagcggc cagaatgtga tgcctgccat catggatgcg
      421 gtgcgcgcgt acgcgacgct gggcgagatc acccagacgt tgaagcaggt cttcggcgtg
      481 tttcgagagc cggtgcggct gtagacccgg catccccatg ccgggaacca gaggagcgag
      541 gaaatcatct atggctacgg gtgtcatggc gcagacggga cctgccctga gcaaagccga
      601 agggttgcag gccgaggcgg cggggcgtgt gcacaacttc aaccccggcc cggccgtttt
      661 gccgctgcct gtgctggaac gggcgcgcga cgaattgctc agctatgacg gcagcggtat
      721 atcggtgcta gagatgagcc atcgatcgcc gcagttcgag ggcatcatgg ctcgcgcgga
      781 agccgatctg cgctcactgt tgaatatccc tgacagctac actgtgttgt ttctgcaggg
      841 tggggcgagt ttgcagtttt cgatggcgcc gttgaacctg cgcgggccgg atcagtcagc
      901 cgactacatc ctgaccggct cgtgggcgaa ggcggcgatc aaagaagccg agaaggcggg
      961 ccgcgttcgc acggctggct cgacggagtc gagcaacttc gattacatcc cgtctcccgc
     1021 cgagctcgat ctggatccgc gggcggccta tcttcacttc accgcgaacg aaacgatcca
     1081 tggcgtcgag tgggccaacg aaccggagcc gccgcctggc gtgccgctcg tgtgcgacgc
     1141 ttcgtcggat atcgccagcc ggccgatcga cgtgtcgagg tacgggctga tctacgcggg
     1201 cgcgcaaaag aacctcgggc cggcgggcgt gacgctcgcc gtcatccgaa acgacttgct
     1261 ggatcgcgtc ccgtcgagac taccggtcat gctcgattac agagcgctcg ccgagagcaa
     1321 gtcgctgcac aacacgccgc cgtgcttttc cgtctatctg gtcgggctgg tgttgcgctg
     1381 gctgaaggac atcggcggct tgcaggcgat cgctcgccac aacgaggcga aggccagact
     1441 ggtttaccag gcgatcgatt cgagcggcgg cttctatcgc ggccacgcgc ggcttgacag
     1501 ccgctcgcgg atgaacgtga cgttccgcct gccgtccgaa gacctggaga agagatttgc
     1561 gaagcaagcc gaggcggagg gattggttgg cctcaacgga catcgctcgg tgggcgggtt
     1621 acgtgcgtcg ctgtataacg ctttgtcgat cgacgccgcc cagtcgctcg tggagttcat
     1681 ggccgagttt cagcgagtca acggatagtc gtcagtaatc agtaatcagt gatcagtaat
     1741 cagtgatcag tgaccagtga ccactaacca ctgatcactg gtcgatgacc attgttctgc
     1801 ggagttcaca atgacgcaca aaatactggt tgctgatggt ctgtccggag acggtattgc
     1861 actgctcagg acggcaggcg aggtcacggt caatccgaag attacggcgg atgaattgct
     1921 ggccgcgctg ccggcgtatc atgcgctcgt cgtccgatcg cgcaccaagg tgacggccca
     1981 ggtcgtcgaa gccgggacga atctcaaggt gatcgggcgg gccggcgtcg gcgtggacaa
     2041 catcgacgtg ggggctgcca tcgcgcgcgg cgttaccgtc gtcaacagcc cgctggccgc
     2101 atcggtcgcc gtcgccgagc acacgcttgg gctgatgctc gcgctagcgc gcaacatccc
     2161 aatcgccgat gcgttgatga agcagggcaa gtgggagaaa aacgcgttcg tgggcagcga
     2221 gctattcggc aaaacactgg gcctgctggg cattgggcgc atcggagccg aagtcgcgaa
     2281 gcgggcggaa gcgttcggaa tgacggtgct ggcctacgat ccttatctca ccgccgagga
     2341 aatccgcgct cgtagggctg tgcctgctgc gtttgacgaa gtgatcgcca aatcggacta
     2401 cctctcgctg cacttgcctc ttactgagga aacgcgtggg ttgattgggc tggagcagtt
     2461 cgcaatgatg aagaaaggcg cgcgcctgat ctgcgctgcc cgggggggcg tgatcgacga
     2521 agacgcgttg cgcgtggcgc tggactcggg cgcgctggcc ggggccgcgc tcgacgtgtt
     2581 cgcatccgag ccgccgctgc cggagagcat tgccgcacac ccaaaggtga ttgctacgcc
     2641 gcacgtcggg gcgcaaacga cagaggctca ggcttccgca gggctaatga tcgccgaaga
     2701 ggtgttggcg gtgctgcagg gacaagggcc gcgctggagg gtgacgacta acgagtgagc
     2761 agttgtcagt aatcagttat cagtaatcag ttatcagtct ggactttgtc cattttcata
     2821 ttgacgccac atataggcat tcattacgac attgtgcccc catatctggg gcgacatgtc
     2881 gcagaaaccg cctaaaacgg gccgaaaagg ccaaattgga caaag
//
LOCUS       DBNHXB010000329         8317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3779342, whole genome shotgun sequence.
ACCESSION   DBNHXB010000329 DBNHXB010000000
VERSION     DBNHXB010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8317
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3779342"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="AC162G_13310"
     CDS             <1..859
                     /locus_tag="AC162G_13310"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR5076830.1"
                     /translation="RMAKEAGLNLAELTGTGPGGRIVRRDVDAAIAAGGAMPAAMPAA
                     PRVPAPAILGPAALKQETIPLTKLRQTIARRMTASKQQAPHFYVTGDIDMAEAIALRA
                     RLNELTDEAGKISVNDMVVKAAALALTHFPNINASFAENAIQRHGAVHVGIAVALDEG
                     LITVVVHDADRKPLAQIARESKDIIARARSGKARPEELQGSTFTVSNLGMFDAESFVA
                     IINPPEAAILAVGSVMDVPVVKDGQLVPGKRMKATISADHRVTDGAEAARFMQVVKKY
                     LEEPLRLLL"
     gene            1005..1352
                     /locus_tag="AC162G_13315"
     CDS             1005..1352
                     /locus_tag="AC162G_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076831.1"
                     /translation="MYPDSEILFPTWCIPILRDLRGEEWRNLVQHVISRPEEHEDTLG
                     FSLMMIRLGGCLTCDLDSYRASLGCATCSKRTVAGFKGTDKALVKKFEEARKEVGAYT
                     GQNGRKQKKPARK"
     gene            1483..2730
                     /locus_tag="AC162G_13320"
     CDS             1483..2730
                     /locus_tag="AC162G_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012377.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HIR5076832.1"
                     /translation="MRGRIMLVLVTVVALVVAGGAWLARDALSSWVFNLTGEESLLGQ
                     IRGLVDLATQFTRPMPNTAPEAVSTPDGISPFGVNTFLHQEVEPAKRARQMQMVADAG
                     FRWIRQPFPWYDIEIQGKGDFADCRHGECVDAWNKYDGIVDLAEKHGVRIVARLDAPP
                     EWARGAPGEFAPPAKFDDYGDFAAAVAGRYRGRVGTYQIWNEPNNYPEWGEQPVDPEA
                     FARLLCTAYRRIKEVDPDAVVIAPALTPTSSLDPGPGPGAGLNDFIFLQRMYHAGAGE
                     CFDVMSAQGYGLFSGPTDDRLRPRVINFGRPQYIRDIMVANGDAGKPIWISEMNWNAV
                     PDEIPDKRFGQVTLDQQARYLPLAYERIRTEWPWVGVAFTWYLKDADDHEKNQAKYYF
                     RLLEPDFTPLPVYEAIKQYASNQ"
     gene            2800..4785
                     /locus_tag="AC162G_13325"
     CDS             2800..4785
                     /locus_tag="AC162G_13325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR5076833.1"
                     /translation="MHARRHTQLILIAILLLAAALRFYRIDAQSFWNDEGNSARIAER
                     GVDLIIAGAAGDIHPPLYYLVLQGWRSVFGNSETALRGLSALFGVLTILFTYLLGSRL
                     FEPRVGLMAAFLAAVNPFLIDYSQEARMYMMLAAIGVVATYLLVRLIDFWSLRPRIHI
                     THRRYYVLYVLALAAGLYTHYAFPFVFIAQFAIVLAWALHRPGRALARLGSWLALAIA
                     AVLLFLPWLPIALRQIAGWPSQTAPIDTGAALVETYRAYLLGRTIPIEQAAMAMIIAG
                     FFLAMSLWTPDAFDEPEPDWDTTAPRALRSGLAAIYLLLPVALVFALGLFRPAYMKFL
                     LVGVPAFCLLLARGIDNAWQIARGALRMPLALRGHSQLAFGWLAIVVMLVTPVLSATA
                     SSLSNLYFNPAYARDDYRGIAQAIRSTWRAGDAVLLHSANQWEVFTYYFPDGPDVFPI
                     VRQRPLDPDATERELNDILATHRRLHAVFWGTDESDPGRWVESWLDKHTYKAGERWYG
                     SARLATYAVPAQVARAPQQALGVRLGDDMWLDGYSLLTPTAAPGDIVQLALFWRGGAP
                     IDERYKVFVHILDGNGNIVAQTDREPGSDLAPTNTWTPGEQVPDRYGVTLPPQLAAGV
                     YPIVVGMYALDGTRLRIFKDGVDQGDALHVAGIEVAP"
     gene            complement(4808..5182)
                     /locus_tag="AC162G_13330"
     CDS             complement(4808..5182)
                     /locus_tag="AC162G_13330"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HIR5076834.1"
                     /translation="MSRFRLASFRFAFEGIVHVLRTQPNARIHAAISIAVVLAGLWLG
                     LDRIGWAILVVTVAVVWVAELVNTAVEAAVDLATVERHPLGKIAKDAAAGGVLAAAIL
                     AVIVGLLILGPPLWARLFGSNY"
     gene            complement(5316..5555)
                     /locus_tag="AC162G_13335"
     CDS             complement(5316..5555)
                     /locus_tag="AC162G_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076835.1"
                     /translation="MTQMEILEELRKLSAAERLKIIEAALRLVREDLQRTEQSTIYSQ
                     KERQLSMAAEALRPDYLAGGELTAFTALDGEDFHA"
     gene            complement(5642..6160)
                     /gene="ybeY"
                     /locus_tag="AC162G_13340"
     CDS             complement(5642..6160)
                     /gene="ybeY"
                     /locus_tag="AC162G_13340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR5076836.1"
                     /translation="MPPPRPRQPNPTPGKGSRLPPRSRFARIDVQVAPRYAGAIRATA
                     IRSAAQAALDAARARPGVSLTIVVTGDAALRRLNREFASVDAPTDVLSFESGDPGEYL
                     GDVMISAPRARAQARRGGHPFIDEMRLLVIHGVLHLLGYDHDTPGRKKRMWAAQAKAL
                     RTVKASIEGPTQ"
     gene            complement(6075..8240)
                     /locus_tag="AC162G_13345"
     CDS             complement(6075..8240)
                     /locus_tag="AC162G_13345"
                     /EC_number="3.1.4.59"
                     /inference="COORDINATES: protein motif:HMM:NF019317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106409 - cyclic-di-AMP phosphodiesterase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD family phosphohydrolase"
                     /protein_id="HIR5076837.1"
                     /translation="MSFGSTSKNRKVRRDWRFWAVGVLLAVGVAAILIVPLFTTDQIS
                     LQVGDVAPRDVKAPRNISYVSEIDTEQARDEAERRVAVVYRPVDPGISRQQVAKARQT
                     IDFISAVRADTITPVDQRRAMLQSVPELSTLTPAQIDNVLTLSDPVWALIADETVAVA
                     DLAMRETIRDVDLAETKTRLPVLVSVSLSEEQAALVSALAQGFIAPNSIRDDEATAQR
                     RDEARNAVESKQVSYIAGQVVMREGAIVTPAMIEALQHMGLATPRVDWTDVAGLALFA
                     LLLASIMGLYLWRFEPELSRQPLSLFLAVVLLLAFILTAKIMVPGRTVLPYVYPAAAL
                     AMLLTVLAGPGLALASSAALAAVIGVMTEGSLELVVYITAGSAVATLTLTGVDRLNGF
                     FWPGVYVGLANIVTILAFRLPGDGLDSVGLLMLTAAALANGILSASITLGVLFLFGNP
                     FDLTTTVQLLELARPTHPLLNELMRKAPGTYHHTLMVANLAEQAAAGIGANALLTRVG
                     AMYHDVGKMVRPYMFVENQMDGSNVHNQLDPQTSAEIIVTHIRDGLELARKYRLPSRV
                     RAFIPEHHGTMRVIFLYNKALERAGGDATKIDEVPFRYPGPKPRSKETALLMLADGAE
                     AATRAARPTSPEQVADIVKKVIGDRIAQGQLDECPLTLNELRLVRDSLTATLQGVFHP
                     RLQYPEPARRADAPAAPAPTESDARERLEAAPAQQVRPN"
BASE COUNT         1481 a         2717 c         2642 g         1477 t
ORIGIN      
        1 acgcatggcg aaagaggcgg ggctgaatct cgccgagctg accggcactg gccccggcgg
       61 gcgcatcgtg aggcgtgacg tcgacgccgc gattgccgct ggcggcgcga tgccggcggc
      121 catgccggcg gcgcctcgcg tcccggcccc ggcgattctc ggcccggccg cgctgaagca
      181 ggaaaccatc cccctcacca aactgcgcca gacgatcgcg cggcgcatga cggccagcaa
      241 acagcaagcg ccgcatttct acgtcaccgg cgacatcgac atggccgagg cgatagcgct
      301 gcgcgcccgg ctcaacgaat tgaccgacga agcaggcaag atcagcgtga acgacatggt
      361 cgtgaaagcg gccgcgctcg cgctgacgca cttccccaac attaacgcct cgttcgccga
      421 gaacgccatc cagcgccacg gcgccgtcca cgtgggcatc gccgtcgcgc tcgacgaggg
      481 tctgatcacc gtcgtcgtcc acgacgccga ccgcaagccg ctggcacaga tcgcgcgcga
      541 gtccaaagac atcatcgccc gtgcgcgcag cggcaaggcg cgcccggagg aattgcaagg
      601 cagcacgttc actgtcagca acctgggcat gttcgacgcg gagagcttcg tcgccatcat
      661 caacccgccc gaggcggcga tcctcgccgt cggctccgtc atggacgtgc cggtcgtcaa
      721 ggatggtcaa ctcgttcccg gcaagcggat gaaggcgacg atctccgccg accaccgggt
      781 gacggatggc gcagaggcgg cgcggttcat gcaggtggtg aagaagtatc tggaagagcc
      841 gttgaggttg ttgttgtgac gaaagctgct cagaggctca agagctacaa cattacataa
      901 atcattaaat tgccctggga attgggagat ttcgggcatt acccttgaat ccgtctttct
      961 tatgtgatat agttcccgcc aactcgagta cgggttgagc ggcaatgtac cccgacagtg
     1021 agatcctctt cccgacctgg tgtatcccta ttcttcgcga cctccgtggc gaagagtggc
     1081 gcaacctggt ccagcacgtg ataagccggc cggaggagca cgaagacacc ctcggcttct
     1141 cgttgatgat gatccggctg ggcggctgcc tgacgtgcga cctcgactcg taccgcgcgt
     1201 cgctcggctg cgccacctgc tcgaagcgca ccgtcgccgg gttcaaaggc acggacaagg
     1261 cgctggtcaa gaagttcgaa gaggcgcgta aggaggtcgg cgcgtacacc gggcagaacg
     1321 gtcgcaagca gaaaaagcca gccaggaagt gaccggcgcc tccgaggcgc cggtcatttc
     1381 tgccaccccc tgattcaccc catcggcagg atgtcgatcg tggtatgatc gcttcacctc
     1441 gccccgcccg gctgctgcgg cgggtctttt cgcggacgct tgatgcgcgg tcgtataatg
     1501 ctggtcctgg ttaccgtggt ggcgctcgtc gtggcaggcg gcgcctggct tgcgcgcgat
     1561 gcgctctcgt cctgggtgtt caacctcacc ggcgaggaat cgctgctggg tcagatacgc
     1621 gggctagtcg atctggcgac gcagttcacg cgcccgatgc cgaataccgc gcctgaggct
     1681 gtatccaccc cggacggcat ttctccgttc ggcgtcaaca cttttctcca tcaggaagtc
     1741 gagccggcca agcgcgcgcg gcagatgcag atggtcgccg acgccggctt ccgttggatt
     1801 cgccaaccgt tcccctggta cgacatcgaa atccaaggca agggcgactt tgccgactgc
     1861 cgtcacggcg aatgcgtcga cgcctggaac aaatacgatg gcatcgtcga tctggctgag
     1921 aagcatggcg tccggatcgt cgcgcggctc gacgcgccgc cggaatgggc gcgcggcgcg
     1981 ccaggcgagt ttgcgccgcc cgctaaattc gacgactacg gcgacttcgc cgccgccgtc
     2041 gccgggcgct atcgcggacg ggtaggcacc tatcaaatct ggaacgagcc gaacaactac
     2101 ccggagtggg gcgagcagcc ggtcgatccg gaggcattcg cccggctgct gtgcaccgcc
     2161 taccggcgca ttaaagaagt cgatccagac gccgtcgtca tcgcgcccgc gctcacgccg
     2221 accagctcgc ttgatcccgg ccccgggcca ggcgccggcc tgaacgactt catcttcctg
     2281 cagcgcatgt accatgccgg ggcgggcgag tgcttcgacg tcatgtcggc gcaagggtac
     2341 ggactgtttt ccgggccgac cgacgatcgg ctgcgaccgc gcgtgatcaa cttcggccgg
     2401 ccgcagtaca tccgcgacat catggtcgcc aacggcgacg cgggcaagcc tatctggatc
     2461 agcgagatga actggaacgc cgtgcccgac gaaattcccg acaagcgctt cggacaggtg
     2521 acgctcgacc agcaggcgcg ctacttgccg ctggcttacg agcgcattcg gaccgagtgg
     2581 ccgtgggtgg gcgtggcatt cacctggtat ctcaaagacg ccgacgatca cgaaaagaat
     2641 caggccaagt attacttccg cctgctcgag cccgacttca cgccgttgcc ggtgtacgag
     2701 gcgataaaac agtatgccag taatcagtaa tcagttgtca gtgatcagtt gtcagtgacc
     2761 actggccact gatcactggt cactgatcac ttgctcatca tgcacgcccg tcgtcatacg
     2821 caactaatcc tgatcgccat cctcctgctc gccgcggcgc tgcgctttta tcgcatcgac
     2881 gcgcagtcgt tctggaacga cgagggcaac agcgcgcgca tcgccgagcg cggcgtcgat
     2941 ctgatcatcg ccggcgcagc gggcgacatt catccgccgc tctactacct ggtgctgcaa
     3001 ggttggcgca gcgtcttcgg caacagcgaa acggcgctgc gaggactttc ggcgcttttc
     3061 ggcgtgctca ccatcctctt cacgtatctt ctcggcagtc gcctgttcga gccgcgcgtc
     3121 gggttgatgg ccgcgttcct cgccgccgtc aaccctttcc tgatcgacta ctcacaggaa
     3181 gcgcggatgt acatgatgct cgccgcgatt ggcgtcgtgg cgacgtatct cctcgtgcgc
     3241 ctgatcgatt tctggagcct gcgcccgcgc atccacatca cgcatcgccg ctactatgtg
     3301 ctgtacgtgc tggcgttggc cgccgggcta tacacccatt acgccttccc gttcgtgttc
     3361 atcgcccagt ttgccatcgt gctggcctgg gccttgcacc ggccgggccg cgcgctggcg
     3421 cgcctgggaa gctggctggc actggcgatc gctgcggttc tcctgttcct gccctggctg
     3481 ccgattgcgc ttcgacagat cgctggatgg ccgtctcaga cggcgcccat cgacaccggc
     3541 gcggcattgg tcgaaaccta tcgagcctac ctgctgggac ggacgatccc catcgagcag
     3601 gcggcgatgg cgatgattat cgcgggtttc ttcctggcga tgagcctgtg gacgcccgat
     3661 gcgttcgacg agcctgagcc tgactgggat acgaccgcgc cgcgcgcgct gcgctccggt
     3721 ttggcggcga tctacttgct gctgcccgta gcgctggtct tcgcgctggg gctgttcagg
     3781 ccggcttata tgaagttcct gctcgtcggc gtgccggcgt tttgcctgct gctcgcgcgc
     3841 gggatcgaca acgcctggca gatcgcgcgc ggcgcgctgc gcatgccgct cgcgctccga
     3901 ggccactcgc aactggcgtt cggctggctg gcgatcgtcg tcatgctcgt cacgccggtg
     3961 ctgagcgcga cggcttcgtc attaagcaat ctatacttca acccggctta tgctcgtgat
     4021 gactatcgcg gcatcgccca ggcgattcgg tcgacgtggc gtgcgggcga cgcggtgctg
     4081 ctccattcgg ccaaccaatg ggaggtgttc acgtattact tccccgacgg cccagacgtg
     4141 ttccccatcg tccggcagcg cccgctcgat cccgacgcga cggagcgcga attgaacgac
     4201 atcctcgcca cgcaccggcg gctgcatgca gtgttctggg gaacggacga gtctgatccg
     4261 gggcgctggg tcgaatcatg gctggacaag cacacgtaca aggcgggcga gcggtggtac
     4321 ggcagcgcgc gtctggcgac gtacgccgtc ccggcgcagg tagcccgcgc tcctcaacag
     4381 gcgctgggtg tacggctcgg agatgacatg tggctcgacg ggtactcgct gctcacgccc
     4441 accgctgcgc ctggcgacat cgtgcagttg gcgctgttct ggcgcggcgg cgctccgatc
     4501 gacgaacgat ataaagtctt tgtgcacatc ctcgatggaa acggcaacat cgtcgcacag
     4561 accgatcgcg agccgggcag cgatctcgct ccgaccaaca cctggactcc cggcgagcag
     4621 gttcccgatc gttatggtgt gacccttccg cctcaactgg ccgcaggtgt gtatccgatt
     4681 gtggtcggca tgtacgccct cgatggcacg cgtctgcgca ttttcaagga cggggtcgac
     4741 cagggcgatg ctctccacgt ggctgggata gaggtcgctc cctgacgcac ggtcctcgtc
     4801 cagcctctca atagttgctg ccgaatagcc gcgcccacaa gggcgggccg aggatgagca
     4861 gtcccacaat caccgccaga atcgccgccg ccagcacgcc cccggcggcg gcgtctttcg
     4921 cgatctttcc cagcgggtga cgctccaccg tcgccagatc gaccgccgct tcgacagccg
     4981 tgttgacgag ctcggcaacc caaacgacgg ccacggtcac caccaggatg gcccacccga
     5041 tccggtccag tcccagccac aagccggcca ggacgaccgc gatcgagatc gccgcatgga
     5101 ttcgggcgtt cggctgcgtc ctcaaaacgt ggacaatacc ctcaaacgcg aagcggaagg
     5161 aggcgagtcg gaagcgggac ataggattat gggagtacgg ggtaggtgcg acgcacctca
     5221 aaggtgcgtc gcacgttgga ggaggcatga ggaatagcag acggtgtgaa agtcaaggaa
     5281 cggttggatc aagattaata agccagacct cgcctctacg catgaaaatc ctcaccatcg
     5341 agtgcggtga aggcagtgag ttcgccgcct gctaagtaat ccggtcgaag tgcttcggcg
     5401 gccatagaga gctggcgctc tttctgagaa taaatggtgg actgttccgt ccgctgaaga
     5461 tcctctcgga cgagccgcag agcagcttcg atgatcttga gacgttctgc agcggacaat
     5521 ttcctcagtt cttccaggat ttccatctgg gtcatagtgc ctccctacca tccattatac
     5581 acaggaatac tacgtcggct agccccagca tcaggcactg aagagacgtt tgccgccctt
     5641 atcactgtgt tggtccctca atggatgcct tcacagttcg cagcgctttc gcctgcgctg
     5701 cccacatgcg cttcttcctg ccaggcgtgt cgtgatcgta ccccagcagg tgcagcacgc
     5761 cgtggatcac cagaaggcgc atctcgtcga tgaatggatg gccgccgcgc ctcgcctgcg
     5821 ctcgcgcgcg cggcgccgag atcatcacgt cgcccaggta ctctccggga tccccagact
     5881 caaaggacag cacgtccgtt ggcgcgtcga cgctggcaaa ctcgcgattg agcctgcgca
     5941 gcgcggcatc gcccgtgacg acgatcgtca gcgacacgcc gggccgcgcg cgcgccgcgt
     6001 cgagcgcggc ctgcgcagct gaccggatgg ctgtcgcccg gatcgcgccg gcgtaacgcg
     6061 gcgcgacctg aacgtcaatt cgggcgaacc tgctgcgcgg gggcagcctc gagcctttcc
     6121 ctggcgtcgg attcggttgg cgcgggcgcg gcgggggcat cggccctcct ggccggttcg
     6181 gggtattgga ggcgaggatg gaacacgcct tgcagcgtcg ccgtgagcga atcgcgcacc
     6241 aggcgcagct cgttcagcgt gagcgggcat tcgtcgagct ggccctgcgc gatgcggtcg
     6301 ccgatcacct tcttgacgat gtcggcgacc tgctccggcg aagtcggccg cgccgcgcgc
     6361 gtcgcagctt cggcgccgtc ggcgagcatc agcagtgcag tctccttgct gcgcggcttc
     6421 gggccgggat agcgaaacgg gacctcgtcg atcttggtcg cgtcgccccc ggcgcgttcc
     6481 agggccttgt tgtacaggaa gatcacgcgc atagtgccgt gatgctcggg aatgaaggcc
     6541 cggacgcgcg acggcagccg gtacttgcgc gccagctcca ggccatcgcg gatgtgggtg
     6601 acgatgattt cggcgctggt ctgcggatcg agctgattat gcacgttcga accatccatc
     6661 tggttctcga cgaacatgta cggccgcacc atcttgccca cgtcgtgata catcgcgccg
     6721 acgcgcgtga ggagcgcgtt ggccccgatg cccgcggcgg cctgttccgc caggttggcg
     6781 accatcagcg tgtggtgata agttcccggc gccttgcgca tcagctcgtt cagaagcgga
     6841 tgggtaggcc gggccaactc gagcaactgc acggtcgtcg tgagatcgaa cgggttccca
     6901 aagaggaaca gcacgcccag cgtgatcgac gccgagagga taccgttcgc cagcgcggca
     6961 gccgtcagca tcagcagccc gaccgaatca aggccgtcgc ctggcagccg gaaggcgagg
     7021 atcgtcacga tattggcgag gccgacgtac acaccgggcc agaagaagcc gttcaggcga
     7081 tcgacgccgg tcagcgtaag cgtggctacg gcgctcccgg cggtgatgta gacgacgagc
     7141 tcgagcgagc cttcggtcat cacgccgatc acggccgcca gcgccgccga cgatgccagc
     7201 gccaagcccg gcccggccag cacggtcagc agcatcgcca gcgccgcggc cggatacacg
     7261 tagggaagca ccgtgcggcc gggaaccatg atcttggcgg tgaggatgaa agccagcagc
     7321 agcaccacgg cgaggaaaag gctgagcggt tggcgactca actccggctc gaagcgccac
     7381 aagtacagcc ccataatcga cgccagcaag agcgcaaaca gggctaaccc ggcgacatca
     7441 gtccaatcga cgcgcggtgt agccagcccc atgtgttgca gcgcctcgat catggcgggt
     7501 gtgacaatcg cgccctcgcg catcacgacc tgacccgcga tgtaggacac ctgcttcgac
     7561 tcgaccgcgt ttcgcgcctc gtcgcgacgc tgggccgtcg cctcgtcgtc acgtatcgaa
     7621 ttcggcgcga tgaagccctg cgccagggcg ctgaccaggg cggcctgctc ctcgctcaaa
     7681 gaaacactga ccagcaccgg caggcgcgtc ttcgtctcag ccagatcgac gtcacgaatg
     7741 gtctcgcgca tcgccagatc ggcgacggcg acggtttcgt ccgcgatgag agcccagaca
     7801 ggatcggaca gggtgaggac attgtcgatc tgggccggcg tgagcgtcga cagctcggga
     7861 acgctttgca gcatcgcacg gcgctggtcg acgggggtga tcgtgtcggc ccgcacggcg
     7921 ctgatgaagt cgatcgtctg ccgggccttc gcgacctgct gccgcgagat accggggtcc
     7981 accggcctgt ataccaccgc gacgcggcgc tcggcctcgt cacgcgcctg ctccgtgtcg
     8041 atctcgctga cataggaaat gttgcgcgga gccttcacgt cgcgtggcgc gacgtcgccc
     8101 acttgcagcg aaatctgatc ggtggtgaag agtggaacga tcaggatagc ggcgacgcca
     8161 acggcgagca gcacgcccac cgcccagaaa cgccaatcgc gcctgacctt gcggttcttt
     8221 gaagttgagc cgaaactcat ccgattccga acacgatctt cccggccaga tcgggatggc
     8281 gcgcgctgct cactgcatca gctcgcgcac gacctcc
//
LOCUS       DBNHXB010000330         4149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4114557, whole genome shotgun sequence.
ACCESSION   DBNHXB010000330 DBNHXB010000000
VERSION     DBNHXB010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4114557"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            437..1636
                     /gene="rpoD"
                     /locus_tag="AC162G_13350"
     CDS             437..1636
                     /gene="rpoD"
                     /locus_tag="AC162G_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR5076838.1"
                     /translation="MATRTKKPATPVIPVIEDSVTTAAEEHLFAIGRERGYVTYDEVL
                     EAFPEAETNLDQLDEVIATLVENGIELGEPPEEEGPEEEDLLDVLDEGTIAAIETDDT
                     VGLYLKEIGHIPLLTADEEVALAKRIEKGLSALKRLSSNGLDAKKRRELEESMRDGRA
                     AREHLITANSRLVISIAKKYIGRGVPFLDLIQEGNIGLIRAARKFDYRRGHKFSTYAT
                     WWIRQAVTRAIADQGRTIRVPVHMGDQINKLIRVSHQLTQELGRDPTPEELADALQIP
                     VKKAEQMIQVARRPISLETPTDEDEDSVLGDFIEDEESVQPSDVVTHNLLKRQIQDIL
                     SALPPREVRILQLRYGLLDGQSYTLEEVGKKLGVTRERVRQIEAQALSRLRHPTHSRK
                     LRDYLRE"
     gene            1853..2068
                     /locus_tag="AC162G_13355"
     CDS             1853..2068
                     /locus_tag="AC162G_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR5076839.1"
                     /translation="MPTYTSVAERSFVAVDYDHCSYCGACVAVCPPDCITLHDATLII
                     DTSACTLCRRCIPACPTGALTMVETVA"
     gene            2065..3240
                     /locus_tag="AC162G_13360"
     CDS             2065..3240
                     /locus_tag="AC162G_13360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5076840.1"
                     /translation="MKSRYDVVVIGAGPAGSIAARTAAEAGLETLLIEKRQEIGSPVR
                     CGEAVGRDTTEKFIPLDPKWIAAEIDCFSIYNVQGDHVVMPPLERTLVLERKIFDREL
                     AHSAAKAGAEVVVKARATGLIRDGGGVEGVRLVLQGEPHEVHATVVIGADGTESQTPR
                     WAGLKSIPQLKNYYTAAQYLMTDIDVNPRICQYHIGWSIAPGGYCWVFPKGDQQANVG
                     LVMWVDPKETKTAIEYLNEFVAARFPSRSILAQVVGGIPITNVLPEMVTDGYLAVGDA
                     AHQSDPLTAGGITNGMYGGLFAAQVAAEGIKKDDVSKKFLKKYEQMWDAEFGKLYRRL
                     LRIRHAVLNVPDDKLGKMIAEASKLDTQMMSLKDIAMIVLKSHPKLLLEAVPYFLGQ"
     gene            complement(3365..>4149)
                     /locus_tag="AC162G_13365"
     CDS             complement(3365..>4149)
                     /locus_tag="AC162G_13365"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5076841.1"
                     /translation="TEVVERSKKIPVLVDFWAPWCGPCRVLSPTLERVAADGDGAFVL
                     AKVNTDENPNLAAQYDVRGIPAVKMFRQGRVVDEFVGVRAEREVRDFIKAFAPSQVDR
                     WMMEAQSLAYGQRWDEAAAAFRRILDAKPDHAEAALELGRMYLAAGRGAEAEAALREV
                     PSSAPEYSTAEALLPLASLIAYAQWPNGSDEGLDRLYRRAGELVLERRAHEAIETLLD
                     VLRKNRNYRDGEAKQALLALFEYLGDDPAVKDYRRQLASVLF"
BASE COUNT          817 a         1305 c         1294 g          733 t
ORIGIN      
        1 ttctattgat aagtcaagtg gatctgtagc tcgtctcgac tctcatggga tccacttggc
       61 ggttgtcgcg atccacgata cactttaagg atgctcaggt cattcgactc gtgcgcgaat
      121 gaatacaagg tcgaggagga tcaggcagcc tgcccggaac ggtgctcccg gtctgtcgat
      181 acagggactg gcccgatttt ggaacacatg ttccatcgcg agatggaggt gggccttttt
      241 cgggcggcca aaggggcatg ggaaccgcaa aatacctggt ttgcgcaatt tgtcgcttga
      301 caaacaaact tttgttgttg tagtatgcta gggactaccc ctgcggctgg attggtccta
      361 tcgggagact cgtagagcac catgtgccgc gctcctggcg tcacgtgtgc tctgtgtgtg
      421 tcgatgagtg aggaaattgg caacgcgaac taagaaaccc gccacaccgg tcattccggt
      481 catcgaagat tcggtcacga cggcagccga ggagcatctg tttgctatcg gtcgcgagcg
      541 cggctacgtg acgtacgacg aggttctgga agccttcccg gaagccgaga ccaacctcga
      601 ccagttggac gaggtgatcg cgacgctcgt cgagaacggc atcgagctgg gcgagcctcc
      661 ggaagaagaa ggcccggaag aagaagacct gctcgacgtg ctcgacgaag gcaccatcgc
      721 cgccatcgaa acggatgaca cggtcgggct gtatctgaaa gagatcggcc acattccgct
      781 gctgaccgcc gacgaggaag tcgcgctggc caagcgtatc gagaagggcc tcagcgcgct
      841 caagcggttg tcgtcgaacg gcctggacgc gaagaagcgc cgcgagcttg aagaatccat
      901 gcgcgacggg cgcgccgccc gagagcatct catcacggcg aactcgcgac tggtcatcag
      961 catcgccaag aagtatatcg gccgcggcgt gccgtttttg gatctgatcc aggaaggcaa
     1021 catcggcctg atacgcgccg cgcgcaagtt tgactaccgg cgcggccaca agttcagcac
     1081 gtacgccacg tggtggattc ggcaggccgt cacgcgcgcg atcgccgacc agggacgcac
     1141 gatccgcgtg cccgttcaca tgggcgatca gatcaacaag ctgatccggg tcagccacca
     1201 gctcacgcag gagttgggcc gcgacccgac tccggaagag ctggccgacg cgctgcagat
     1261 cccggtgaag aaggccgagc agatgatcca ggtcgcgcgg cgcccgatct cgctcgagac
     1321 gccgaccgac gaagacgaag actcggtgct gggcgatttc atcgaggacg aggaatcggt
     1381 tcagccttcc gacgtggtca cccacaacct gctcaagagg cagatccagg acattctatc
     1441 cgccctgccg ccgcgcgaag tgcgcatcct gcagttgcgg tacggcctgc tcgacgggca
     1501 gtcgtacacg ctcgaggaag tcggcaagaa gctgggcgtc acgcgcgagc gcgtgcggca
     1561 gatcgaagcg caggcgctgt cgcgcctgcg ccatcccaca cactcgcgca agctgcgcga
     1621 ctacctccga gaatagccct tagaccccgg cactttgccg gggtcttctt ttttcttcca
     1681 cgaattcaca cgaaatcgcg cggatttgtg cggattcctg aacaacccca aagcgcgggt
     1741 gagcagttac gccagttgac gcaaaccgga tgtctcatct ataatccacg catccgcata
     1801 agctctggct tctgacaact taacgtttac ccgcagggat cggagacccg gtatgcccac
     1861 ttacacatcc gttgcggagc gttctttcgt cgccgtcgat tacgatcatt gctcctattg
     1921 cggcgcatgc gtggccgtgt gcccgcccga ctgcatcaca ctgcacgacg ccaccctgat
     1981 tatcgatacg agcgcatgca cgctgtgccg gcggtgtatc cccgcctgcc cgaccggcgc
     2041 gctgacgatg gtggagaccg tcgcatgaag agtcgctacg acgtcgtcgt catcggcgcc
     2101 gggccggccg ggtcgatcgc cgcgcgcacg gcggcagaag ccgggttgga gacgctgctg
     2161 atcgagaaac ggcaggagat cggctcgccc gtgcgctgcg gcgaagccgt aggacgcgac
     2221 acgaccgaga agttcatccc gctcgatccg aaatggatcg ccgccgagat cgattgcttc
     2281 agcatctaca acgtgcaggg cgatcacgtc gtgatgccac cgctggagcg cacgctcgtg
     2341 cttgagcgca agatattcga ccgcgagctg gcgcattcgg cggcgaaggc cggcgcggag
     2401 gtcgtcgtca aggcgcgcgc gaccggcctg atccgagatg gcggcggggt cgaaggcgtg
     2461 aggctggtcc tgcagggcga gccacacgag gtacacgcga cggtcgtcat cggcgcggat
     2521 ggcaccgagt cgcagacgcc gcgctgggcc ggcttgaagt cgatcccgca gctcaagaac
     2581 tattataccg ccgcgcagta cctgatgacc gacattgacg tgaacccgcg catttgccag
     2641 tatcacattg gatggtcgat cgcgcccggt ggctactgct gggtcttccc caagggcgac
     2701 caacaggcga acgtcgggct ggtgatgtgg gtcgatccga aggagacgaa aacggcgatc
     2761 gaatacctca acgaattcgt cgccgctcgc tttcccagca ggtcgatcct ggcgcaggtc
     2821 gtcggcggca tccccatcac gaacgtgctg ccggagatgg tcacggacgg ctacctggcc
     2881 gtcggcgacg cggcgcacca gagcgatccg ctcacggcgg gcggcatcac gaatggcatg
     2941 tacggcggcc tgttcgccgc gcaggtcgcc gccgagggga tcaagaagga cgacgtctcg
     3001 aagaagtttc tgaagaagta cgagcagatg tgggacgccg agttcggcaa gctctatcgc
     3061 cgcctgctgc gcatccggca cgccgtcctg aatgtgcccg acgacaagct cggcaagatg
     3121 atcgccgagg cgtcgaagct ggatacgcag atgatgtcgc taaaggacat cgcgatgatc
     3181 gtgctgaagt cgcacccgaa gctgctgctc gaagccgtgc cgtatttcct ggggcagtag
     3241 tgatccagac ttcggaagtc ttgaagcgaa ccattgcagg cgaggatgcc tgaccatcaa
     3301 agtagcctcg ctggcgaggc agcgcctgaa gacttccgaa gtctttcagg agacccgtga
     3361 ggtcttagaa caacacgctc gccaactggc gacggtagtc tttcactgcc gggtcgtcgc
     3421 ccagatattc gaacagcgcc aggagcgcct gtttcgcttc gccgtcgcga tagttccgat
     3481 tcttcctcag cacgtcgagc agcgtttcga tcgcctcgtg cgcgcgccgc tccaggacca
     3541 gctcacccgc ccgccgatat aacctgtcga gtccctcgtc cgacccattc ggccactgcg
     3601 cataggcgat gagcgaggcc agcggcagca aggcttcggc agtcgaatat tccggcgcgc
     3661 tcgacggcac ttcgcgcagc gccgcctcgg cctctgcccc ccgccctgct gccaggtaca
     3721 ttcggccaag ctcgagcgcc gcttctgcgt ggtcgggctt cgcgtcgagg atgcgccgaa
     3781 acgccgccgc cgcctcgtcc cagcgctgcc cgtaggcgag cgactgcgcc tccatcatcc
     3841 aacgatcgac ctgcgacggc gcaaacgcct tgatgaaatc gcgcacttcg cgctcggccc
     3901 tcacgccgac gaactcgtcg acgacgcgcc cctgccggaa catcttgacc gccgggatgc
     3961 cgcgcacgtc atactgcgcc gccaggttcg ggttctcgtc ggtgttcacc ttcgccagga
     4021 caaacgcgcc atcgccgtct gcggcgacgc gctcgagagt ggggcttaag acacggcacg
     4081 gcccgcacca cggcgcccag aaatccacca ggacggggat tttcttcgag cgctcgacga
     4141 cttcggttt
//
LOCUS       DBNHXB010000331         9047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2270645, whole genome shotgun sequence.
ACCESSION   DBNHXB010000331 DBNHXB010000000
VERSION     DBNHXB010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9047
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2270645"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="AC162G_13370"
     CDS             <1..1352
                     /locus_tag="AC162G_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HIR5076842.1"
                     /translation="KGKVVLATVYGDVHDIGKSLVNTILTNNGFTVFDLGKQVPANVI
                     IDKAIEVEATAIGLSALLVSTSKQMPLIVNELHRRGLAFPVLIGGAAINRKFGRRILF
                     AESGQPYTPGVFYCKDAFEGLETMEKLIDPNERGDFVGRIIAEARAEVENQRLEIRDS
                     GPSLQSRISTVPRLPAKEIPMPPFWGARVIRQMPLEIVLQFLDLDELYRLQWGAKNAH
                     GEDWTRLKAEFDARLDRMRREALKLKVLEPRAVYGYYPAQSDGDSLIIYDPGPWMNHP
                     GGNGKKPLREIARFTFPRQPDRDRLCLSDYFAPADSGVVDVAALQVVTVGPKAEQRFA
                     ELQGRGDYSEAYFTHGLSVEAAEATAEYVHRHIRRELGIDAKRGKRYSWGYPACPDLG
                     DHVKVFELLPAEKELGMQLTAAYQLVPEHSTAAIVVHHPAARYYSVGVSRVEQLMAN"
     gene            1389..1754
                     /locus_tag="AC162G_13375"
     CDS             1389..1754
                     /locus_tag="AC162G_13375"
                     /inference="COORDINATES: protein motif:HMM:NF013561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HIR5076843.1"
                     /translation="MRENMVRTQVYLPRKIYDELQARAEKQGLTLAVQIREALEDYIG
                     RVEAEADDGILHADDPIFQLIGAASSREGDLSVNHDHYLYGWPKRELIDEPAEVRVKE
                     RRSRYQTPRKKSPSRRRRK"
     gene            1751..2158
                     /locus_tag="AC162G_13380"
     CDS             1751..2158
                     /locus_tag="AC162G_13380"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR5076844.1"
                     /translation="MILFGDSSGWVAAYDERDKYHAAAKQIIYDLSGQKVTFVVTDYV
                     VAESITLMRSRLGHARAVAFGDWLLQSPRVRLIRLDLDLWNEAWRFFKMYDDKDFSFT
                     DCASFVVMRRERLRDAFTFDRHFEQAGFRLWPR"
     gene            2170..3174
                     /locus_tag="AC162G_13385"
     CDS             2170..3174
                     /locus_tag="AC162G_13385"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5076845.1"
                     /translation="MPTTLVMETFSSRALANNPLGDPATRKLPVILPPDYETSGKRYP
                     VIYGLTGFTGSGPMLLNVTAWQPNLQERIDTLMADGRLPPAIFALPDCFTRYGGSQYI
                     NSTAIGRYEDYLIEEIVPFVDSKYRTIAAPEGRGLFGKSSGGYGAIVLGMRHPDVFGA
                     LACHSGDMYFEMCYKPDFPKFINAVLKAGGLEKWWAEFEKKVKKERQDFEAVNILAMA
                     AAYSPDPDRKPLPVDFPFDMYTGELRPDVWQRWLDHDPVYLARRYADNLKKLRLLYLD
                     CGTRDNFNLHFGARILVERLKSLGVPHEHEEFDDDHMNIAYRYDVSLPKLGRALDPAT
                     "
     gene            3307..4092
                     /locus_tag="AC162G_13390"
     CDS             3307..4092
                     /locus_tag="AC162G_13390"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR5076846.1"
                     /translation="MPRFDANYVPKSTLTIFAHPDDAEFGVAGTLARWAKAGCEITLV
                     LCTSGNVGTHDGKYTRESLAETRKKEQKAAAQLLGVKHVVFLDHDDCELQPTLELRRE
                     LVREIRKHKPEVVISGRPDGWLYGNEYINHPDHRAAAAATLEAVFPCAEMELLWPEEG
                     QAHKVHAVYVTYTEEADIWIDITDTIDAKIASLRAHASQMGEWDPEPMIREWAAGDAK
                     AGRKKMKPAKDKKATKGKKNKMKYAEAFRVMKLVNDEQKPPES"
     gene            4241..6127
                     /locus_tag="AC162G_13395"
     CDS             4241..6127
                     /locus_tag="AC162G_13395"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HIR5076847.1"
                     /translation="MTTGFLDRLRYAPMLADGAMGTEIFARGVTFDRCFDELNLSQPD
                     AILDIHRRYVEAGAEVLETNTFGANHFRLIEHDLPAKVAEINVAGVRLARMAADESRR
                     RVYVAGSVGPLGVRLAPLGRVQPAQARQAFLAQIRALREAGVDLLILETFSDLNEIRE
                     AILAAKEVAPDVPLVAQMTFTEDGRTPLGDSAETVARVLLELKADVIGANCSVGPARL
                     LPVVNAMANLAGGAPVSVMPNAGWPQRAGPRIIYPATPDYFADFTRRFLFAGAALIGG
                     CCGTTPKHIRVMRAALDAAIVHRISAGPGSPVEVISRASLSEPPGREEPYPPTRLQEK
                     LARGDFVVAVEVDPPKGHNAGRILEAARELGKAGVDVIHVADTPMARMRMSAWALAHL
                     VQRETDLETVLHFPTRGRNLLRVQGDLLAAHALGVRNLFVAMGDPPAVGDYPEAGDQL
                     DIVPTGLVRMIKENFNTGRDHSGKSIGGPTGFFVGVALSFHAPDIEREMRVLRKKIAS
                     GADFVVTQPVFDVEPARRFLEGYRDRYGEIPIPIIAGVLPPASRRNAEFLHNEVPGIR
                     LPDWVLERMRGAQDERREGIRIAQEVLEQLRPLVQGVYVMPMLGRFDTAAEVIEVVKA
                     RAAI"
     gene            complement(6190..7506)
                     /locus_tag="AC162G_13400"
     CDS             complement(6190..7506)
                     /locus_tag="AC162G_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017745371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5076848.1"
                     /translation="MVRKNSSLGVRVFALVWLGQLVSLTGSGLTDFGLSVWVFQHTGS
                     TTQSALTFLFFTVPRILLSPLAGTLVDRWDRRRAMLLSDSGAALVTLSIALLLLADQL
                     EVWHVYAATAAGSAFGAFQRPAYRATITLLIPKQHYGRASGLVGLARSIPSLVSPILA
                     GLLIVAVGLGGVLLIDFVTYLFAALTLLIVRFPRPERSAESEAAQGSLMREAAAGWAY
                     IARRPGLLGLMIFAAATHFLGVTTEVLLTPYMLSFTTADALGFVVSATGAGLLAGGFV
                     MSAWGGPERRIHGILGFELLIAACTILMGLRASPILIGASAFVYFAGVELADGSTYAL
                     WQTKVAPDLQGRVFAMHDMIALLALPLGILITAPLAEFVFEPMLRDGGALAGSVGQII
                     GVGPGRGIALVFLLTGLFNTLAVIAAAFHPRIRRIDDELPDAIQGR"
     gene            7686..9014
                     /locus_tag="AC162G_13405"
     CDS             7686..9014
                     /locus_tag="AC162G_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR5076849.1"
                     /translation="MMQAFYWDCPREDKREFEWWNYVREKVATLAQLGFASLWLPPAH
                     KAANLSGPSMGYDPYDYYDLGEFDQKGSVKTWFGSRRELLDLIHTAHHHGMSVIADMV
                     INHNGGADAAEVNPITGQTRWTLFRPHSGKFPRNWECFHPNPYESWDESTFGDLPDLS
                     HRNPYVFGEILKLARWLIEDIGFDGFRYDFVKGYGATTVTAIQEYRYMRDGQPFRPYG
                     VAEHWDSARSIEYWVNVTNFSNQNPVDAFDFPLREMLKALCDEYGFSLHKLVTWETLL
                     QKQPQTTVTFVENHDLRDWGRPIVNDKLLAYSYILTHEGYPCVFWKDYYNYGLALETT
                     PHGIAALVQAHEHYAGGGTQVLWLEDDLYIMQRSGYADKPGLIYVLNNRGDRWKGRWV
                     ITQWRNVEFIPVAWWSKADRNRPRPQYAHADARAQFWAPPRGYVVYAPRV"
BASE COUNT         1826 a         2766 c         2887 g         1568 t
ORIGIN      
        1 cgaagggcaa ggtcgtgctg gccacggtgt acggcgatgt gcacgacatc ggcaagagcc
       61 tcgtcaacac gatcctgact aacaacggct tcaccgtgtt cgacctgggc aagcaggtgc
      121 cggccaacgt gatcatcgac aaggcgatcg aagtcgaggc cacggcgatc ggcctgagcg
      181 cgctgctcgt atccacgtcg aagcaaatgc cgctgatcgt caacgagctg cacaggcgcg
      241 gcctggcgtt ccccgtgctc atcggcggtg cggcgatcaa tcgtaagttc ggccgcagaa
      301 tcctgttcgc cgagtcgggg cagccgtaca cgccgggcgt gttctactgc aaagacgcgt
      361 tcgagggcct ggagacgatg gagaagctga tcgaccccaa cgagcgcggc gacttcgtgg
      421 ggcggataat cgcggaggcg agggcggaag tcgagaatca gagattggag attagagatt
      481 caggccccag tctccaatct cgtatctcta ctgttccgcg tttgccggcg aaggaaatac
      541 ccatgccgcc gttctggggc gcacgtgtca ttcgccaaat gccgctcgag atcgtgctgc
      601 agtttctcga cctggacgag ctgtatcgtc tgcagtgggg cgcgaaaaac gcgcatggtg
      661 aagactggac aaggctgaag gccgagttcg atgcacggtt ggatcgcatg aggcgtgaag
      721 cgctgaagct gaaggtcctc gagccgcgcg ccgtgtacgg ctactacccg gcgcagtccg
      781 atggcgactc gctcatcatt tacgaccccg gcccatggat gaatcatccg ggtggcaacg
      841 ggaagaagcc tctccgcgag atcgcccggt tcaccttccc gcgccagccc gatcgcgatc
      901 ggttgtgcct atccgactac ttcgcgccgg ctgactcagg cgtcgtcgac gtggcggcgc
      961 tgcaagtcgt gaccgtcggc ccgaaagcgg agcagcgctt cgcagagctt caaggccgag
     1021 gcgattacag cgaggcgtac ttcacacacg gcctgtcggt cgaagcggcg gaggcgacgg
     1081 ccgaatacgt gcatcgccac atccggcgag aactgggcat tgacgcgaag cgcggcaagc
     1141 gttactcgtg gggctacccg gcctgcccgg acctcgggga tcacgtcaag gtgttcgagc
     1201 ttctgcctgc cgaaaaggag cttggcatgc agttgacggc ggcgtaccag cttgtgcccg
     1261 agcattcgac ggcggcgatc gtcgtgcacc acccggcggc caggtactac tcggtggggg
     1321 tgagccgggt ggagcaactc atggcaaatt agatgtataa tatatacatc tgatttcgga
     1381 gtgttgctat gcgcgaaaac atggtgagga ctcaagtata tctgccgcgt aagatctacg
     1441 acgagctaca ggcacgggcc gagaaacagg gtttgacgct ggcggtgcag atccgcgaag
     1501 cgctggagga ttatataggg cgtgtagagg ccgaagccga cgacgggatt ctgcatgcag
     1561 acgatccgat ctttcagttg atcggagcag cctcaagccg ggagggagac ctgtctgtca
     1621 atcacgacca ctacctttat ggatggccta agcgcgaatt gatagacgag cctgccgaag
     1681 tccgggtgaa agaacgccgc tcgcgttatc aaacaccccg caagaagtct cccagccggc
     1741 ggcgaagaaa atgatcctgt ttggagacag ttcaggctgg gtagccgcat atgatgagcg
     1801 cgataagtat cacgccgccg caaagcagat tatctacgat ctatctggcc agaaagtgac
     1861 tttcgtcgta actgactatg tggtggccga gtcaatcacg ttgatgcgat cccgattagg
     1921 acatgcgcga gccgttgcat tcggcgactg gttgttacag tcgccgcgcg tcaggctgat
     1981 caggctggat ctcgatctgt ggaacgaagc gtggcggttt ttcaagatgt acgacgataa
     2041 ggatttctcc ttcaccgatt gcgccagttt tgtcgtcatg cgccgcgaac ggctgcgtga
     2101 tgccttcaca ttcgatcgcc atttcgagca ggccggcttt cgtctttggc cgcgctagag
     2161 gattcaccta tgcccacgac tctcgtcatg gaaacgtttt ccagccgcgc cctggccaat
     2221 aacccgttgg gagacccggc gactcgcaaa ttgcccgtca tcctgccgcc ggattacgag
     2281 acgagcggca agcgttaccc ggtcatctac ggcctgactg gctttaccgg cagcggcccc
     2341 atgctgctga acgtcaccgc ctggcagccg aacctgcagg agcgcatcga caccctgatg
     2401 gccgacgggc gcctgccgcc cgcgatcttc gccttgcccg actgcttcac acgctacggc
     2461 ggcagccagt acattaactc gacggcgatc ggacgctacg aggactatct catcgaagag
     2521 atcgtcccgt tcgtcgattc gaaatatcgc acgatcgccg cgccagaggg ccgaggtttg
     2581 ttcggcaagt cgagcggcgg ctacggcgcg atcgtgctgg gcatgcgcca tcccgacgtg
     2641 ttcggcgcgc tcgcctgcca cagcggcgat atgtacttcg agatgtgcta caagccggac
     2701 ttccccaagt tcatcaacgc cgtgctcaag gcgggcgggt tggagaagtg gtgggccgag
     2761 tttgaaaaga aggtcaagaa ggagcggcag gacttcgagg cggtgaacat cctggcgatg
     2821 gcggctgcct actcgcccga tcccgaccgg aagccgctgc ccgtcgactt cccgttcgat
     2881 atgtacaccg gcgagctgcg gcccgacgtg tggcagcgct ggctggatca cgatccggtg
     2941 tacctggccc ggcgctatgc cgacaacttg aagaagctgc gcctgctgta cctcgactgc
     3001 ggcacgcggg acaatttcaa cctgcatttc ggcgcccgca tcctcgtcga gcgcctgaag
     3061 tccctgggcg tgccgcacga gcacgaagag ttcgacgacg accacatgaa catcgcgtat
     3121 cgttacgacg tgtcgctgcc gaagctcggc cgtgcgctcg acccggcgac ctgaatcgtg
     3181 aagcgtgatg cgtgatgcgt gaagcttgat gagtgacaag atgtgataag ccatatgcga
     3241 tacgcgataa gcgatatgcc ataagccatc tgctatcagc gatccgccat cagccaaggg
     3301 gagaacatgc ctcgcttcga cgcaaactac gtcccgaagt ctactctgac catcttcgcc
     3361 catcccgacg atgccgagtt cggcgtcgcc gggacgcttg cccgctgggc gaaggccggc
     3421 tgcgagatca cgctcgtgct atgcaccagc ggcaacgtcg gcacgcacga cggcaagtac
     3481 acgcgcgagt cgctggccga gacgcgcaag aaggagcaga aagctgccgc ccagctcctc
     3541 ggcgtgaaac atgttgtctt cttagatcac gacgactgcg agctgcagcc gacgctcgaa
     3601 ttgcgccgcg agctggtgcg cgagattcgc aagcacaagc ctgaggtcgt gatcagcggc
     3661 cggcccgatg ggtggctcta cggcaacgag tacatcaacc atcccgatca ccgcgcggcg
     3721 gcggcggcga cgctcgaggc cgtgttcccc tgcgccgaga tggagctgct gtggcccgaa
     3781 gagggccagg cgcacaaagt gcatgccgtg tacgtcacct acacggagga ggccgacatc
     3841 tggatcgaca tcaccgacac gattgacgct aagatcgcgt cgctcagggc gcacgccagc
     3901 cagatggggg agtgggatcc ggagccgatg atccgcgagt gggcggcggg tgacgccaag
     3961 gcgggccgta agaagatgaa gccggcgaag gacaagaaag ccacgaaagg caagaaaaac
     4021 aagatgaaat acgccgaagc gtttcgggtg atgaagctgg tcaacgacga gcaaaaaccg
     4081 cccgaatcct gatcagtgct cagtgaccag caaacagtga ccagtgatca gtaatcagtg
     4141 atccgtagtc agtgacacga ctggtcactg gtcactgatc actgacaact gacgactgac
     4201 aactgatgac tgataactga aaactgcaca gagaccaccc atgaccactg gcttcctgga
     4261 taggcttcgt tacgcgccaa tgctggccga cggcgcgatg gggacggaaa ttttcgcgcg
     4321 cggcgtcaca ttcgatcgct gcttcgacga gctgaacctg tcccagccgg atgcgatcct
     4381 cgacatccac cggcgttacg tcgaagctgg cgccgaggtg ctggagacga acaccttcgg
     4441 cgcgaatcac tttcgcctga tcgagcacga cttgccggcg aaggtcgccg agatcaatgt
     4501 ggccggcgtg cggctggcgc gcatggccgc cgacgagtca cgccggagag tgtatgtcgc
     4561 tggatcagtc ggcccgctcg gcgtgcggct cgcgccgctc ggccgtgtgc agcccgcgca
     4621 ggcgcgccag gctttcctcg cccagattcg ggcgctgcgc gaggcgggcg tcgatctcct
     4681 gatcctcgag acgttcagcg acctgaacga gatccgtgaa gccattctgg cagcgaaaga
     4741 ggtcgcgccc gacgtcccgc tcgtcgcgca gatgaccttc accgaggacg gccgtacgcc
     4801 tctgggcgac tcggccgaga ccgtggcgcg ggtgctgctc gagctcaaag ccgacgtgat
     4861 cggcgcgaat tgcagcgtcg ggccggcgcg gctgctgccg gtcgtcaacg cgatggcgaa
     4921 cctggcgggc ggtgcgccgg tcagtgtgat gccgaacgca ggctggccgc agcgcgccgg
     4981 gccgcgcatc atctaccccg ccacgccaga ctacttcgcc gatttcacgc gccgtttcct
     5041 gttcgccggc gcggcgctca tcggcggttg ctgtggcacg acgccgaagc acatccgcgt
     5101 gatgcgcgcc gcgctcgacg cggcgatcgt gcaccgcatc agcgccgggc ctggttcgcc
     5161 cgtcgaggtg atcagtcgtg catccctttc agagccgccg gggcgcgagg agccgtatcc
     5221 gccaacacgc ctgcaggaga agctcgcgcg gggcgacttc gtcgtcgccg tcgaggtcga
     5281 tccgcccaaa ggccacaacg ccgggcgcat tctggaagcg gcgcgcgagc tgggcaaagc
     5341 gggcgtcgac gtgattcacg tcgccgacac gccgatggcg cgcatgcgca tgagcgcctg
     5401 ggcgctggcg cacctggtgc agcgcgagac tgatctcgag accgtcctgc acttcccgac
     5461 gcgcggtcgt aacttgctgc gcgtgcaggg cgacctgctc gccgcgcacg cgctcggcgt
     5521 gcgcaacctg ttcgtcgcga tgggcgatcc gcctgccgtc ggggactatc ccgaggccgg
     5581 cgatcagctc gacatcgtgc cgaccggctt ggtcaggatg atcaaagaga acttcaacac
     5641 cggtcgcgat cattccggca agtcgatcgg cgggccgacc ggctttttcg tcggcgtggc
     5701 gctcagtttc catgcgcccg atatcgagcg cgagatgcgc gtgctgcgca agaagattgc
     5761 gagcggcgcc gacttcgtcg tcactcagcc ggtgttcgac gtcgagccgg cgcgccgctt
     5821 cctggagggc tatcgcgatc ggtacggcga gattccgatc ccgattatcg ccggcgtgct
     5881 gccgccagcc agccggcgca acgccgagtt tctgcacaac gaagtgccgg gcatccgcct
     5941 gcccgactgg gtgctggaac gcatgcgcgg cgcgcaggac gagcggcgcg aaggcatccg
     6001 catcgcgcag gaggtgctcg aacaactgcg gccgctcgtg cagggcgtgt acgtcatgcc
     6061 gatgctcggc cgcttcgaca cggcggcgga ggtgatcgag gtggtgaagg cgagagcggc
     6121 aatttgatcc gctctgccaa ccttgcgaag gttgcagacg ctcgtgaggt taaagcctgc
     6181 ctggcaaatt caacggcctt ggatggcgtc gggtagctcg tcgtcaatcc gtcgaatgcg
     6241 gggatggaag gcggccgcga tcacggccag ggtgttgaac aggccagtga gaaggaacac
     6301 cagcgcgata ccccgccctg gcccaacgcc gatgatctga cccacgctgc cggccagcgc
     6361 accgccgtcc ctcagcattg gctcgaacac gaactcggcc agcggcgcgg tgatgaggat
     6421 acccagcggc agggccaaca gcgcgatcat gtcgtgcatg gcgaacacgc gtccctggag
     6481 atcgggcgcg accttggtct gccacagcgc gtaggtgctg ccgtcggcca gttcgacgcc
     6541 ggcgaaatag acgaacgcgg aggcgccgat caggatcggc gatgcgcgca gccccatcag
     6601 gatcgtacac gcggcgatca gcagctcgaa gccgagaatg ccgtgtatcc gccgctcagg
     6661 cccaccccag gcactcatga cgaagccgcc agccagcagt cctgcgccgg tcgccgacac
     6721 gacgaatccc agcgcatcgg ccgtggtgaa cgagagcatg tagggcgtca gcaagacctc
     6781 ggtcgtaacc cccaggaagt gcgtggcggc agcgaagatc atcagaccca gcaagccggg
     6841 ccggcgagcg atgtacgccc agccggcggc cgcttcgcgc atcagcgagc cttgtgccgc
     6901 ctcgctctcg gcgctgcgct cgggccgggg aaagcggacg atgagcagcg tgagcgcggc
     6961 gaacaggtag gtgacgaagt cgatcaggag cacgcctccc aacccgaccg ccacgatcaa
     7021 taaaccggcc aagatcgggg agaccagcga tgggatcgac cgcgccaggc cgactagtcc
     7081 gctggctcgc ccgtagtgtt gtttgggaat gagcaacgtg atcgtcgcac ggtaggcggg
     7141 ccgctgaaaa gcgccgaacg ccgaaccggc tgcagtcgcc gcgtagacgt gccagacctc
     7201 cagttggtcg gccagcagga gcagcgcgat cgacagcgtg acgagcgctg ccccggagtc
     7261 gctgagcagc atcgcgcggc ggcggtccca gcgatcgacg agtgtcccgg ccagcggcga
     7321 gaggaggatg cgcgggactg tgaagaacaa gaacgtcagc gccgactgag tcgtcgagcc
     7381 ggtgtgctgg aagacccaca cgctcaggcc gaagtcggtg aggcccgaac cggtcagcga
     7441 gacgagctgc cccaaccaga cgagggcgaa gacccgcact ccgagggatg aattctttct
     7501 caccataccc ttcacgccta aaatgcctga cgttccccag cggtgttttt tccaaacgct
     7561 tagagcattg tacagcgaag catacgccga cgaaaatgat ttggttgact gggaggacaa
     7621 cacacttgac ttggaggatg ggttgtcata gactagagaa gagaaagggc ctgagcatgg
     7681 ctgtcatgat gcaagcattt tactgggatt gtccccgtga ggataaacgg gaattcgaat
     7741 ggtggaacta cgtgcgtgag aaggtagcca ccctcgctca gttgggtttt gcgtccttgt
     7801 ggctgccgcc ggcgcacaaa gccgctaacc tctccgggcc gtcgatgggc tacgatccct
     7861 acgattatta cgatctgggt gagttcgacc agaaaggctc ggtcaagacc tggttcgggt
     7921 ccaggcgaga actgctcgac ttgattcaca ccgctcacca tcacgggatg agtgtgatcg
     7981 ccgacatggt catcaaccac aacggcggag cggatgcggc ggaagtcaac ccgattaccg
     8041 gccagacccg ttggacgctc ttccggcccc acagcggcaa gttcccgcgc aattgggaat
     8101 gcttccatcc caacccatac gagagctggg acgagagcac cttcggcgac ctgcccgatc
     8161 tttcccatcg caacccttac gtcttcggcg agattctgaa gctggcacgc tggctcatcg
     8221 aagacatcgg tttcgacggt ttccgctacg atttcgtcaa gggctatggc gcgacgacgg
     8281 tgacggccat ccaggaatac cgctacatgc gggatgggca acccttcagg ccgtacggtg
     8341 tcgctgagca ctgggacagc gcccgctcca tcgaatactg ggtcaacgtg accaactttt
     8401 ctaaccagaa ccccgtggat gccttcgatt ttcctctgcg tgaaatgctc aaagccttgt
     8461 gcgacgaata cggcttcagc ctgcacaagc tggtgacgtg ggagacgctg ctccagaagc
     8521 agccacagac gacggtcacc ttcgtggaga accacgatct gcgcgactgg ggccgcccca
     8581 ttgtcaacga caagctactt gcctatagct acatcctgac acacgagggc tacccctgtg
     8641 tcttctggaa agactattac aactatggcc tggcgctgga aacgacgccg catggcatcg
     8701 ccgccctcgt tcaggcacat gagcattatg cagggggcgg aacgcaggtt ctctggctag
     8761 aggatgatct atacatcatg cagcgcagcg gttacgccga caaacccggc ttgatttatg
     8821 tcctcaataa ccgaggcgac cgctggaagg ggcgctgggt gatcacacag tggcggaatg
     8881 tcgaattcat tcccgtcgca tggtggagca aagccgaccg gaaccgaccg aggccccaat
     8941 acgctcacgc cgatgcgcgc gcccagttct gggccccgcc gcgcggttac gtggtctatg
     9001 cgccgcgggt ttaggtgagc acgcacacca aagagtatct cacgcat
//
LOCUS       DBNHXB010000332        23077 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_425483, whole genome shotgun sequence.
ACCESSION   DBNHXB010000332 DBNHXB010000000
VERSION     DBNHXB010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 23077)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 23077)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23077
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_425483"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="AC162G_13410"
     CDS             complement(<1..447)
                     /locus_tag="AC162G_13410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5076850.1"
                     /translation="MSAATTAPARRIHYGWVILAVSVPVVFGSLGLARFGYSVILPSM
                     QASLELSNAQAGGLATANLVGYLAFSIIGGALAARFGPRRIVTVGLVLAGLGMALTGL
                     AQAFLPAAIWRALTGVGSGLSNVPVMGLLSAWFAARRRGLASGIAVM"
     gene            complement(447..1757)
                     /locus_tag="AC162G_13415"
     CDS             complement(447..1757)
                     /locus_tag="AC162G_13415"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR5076851.1"
                     /translation="MPIWNPEFESMPRDQLNKVQARRLRDVVARAAASVPFYKKKFAE
                     AKVSADDLQSLDDLKRPPFTEKADLRDNYPFGMFTVPLDQVVRIHASSGTTGKPTVGG
                     YTRADIDLWTEVMARTVTCAGVTSKDVVHNAYGYGLFTGGLGFHLGAEKVGAMVVPIS
                     GGLTRRQVMLMEDFAATVLTCTPSYALVIGEEAAEMGLDFKKRMKVRVGIFGAEPWSD
                     KMRTEIESKLGLKAYDIYGLTEIIGPGVSIECEHNCGMHIQEDHFLAEIIDPETGEQL
                     PYEEEGELVFTTLTKEAMPVIRYRTRDRTRLHADKCACGRTTVRMDKVLGRTDDMLIV
                     RGVNVYPQLIEKTLLSVEDIEPHYQIVLDRPKDQLDTLEVWVEASPKLFQPIDTRRLD
                     ELQNRVSAELAQTLGISAVVRLMGPRSIQRYEGKAKRVIDKREL"
     gene            complement(1776..2366)
                     /locus_tag="AC162G_13420"
     CDS             complement(1776..2366)
                     /locus_tag="AC162G_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HIR5076852.1"
                     /translation="MVTANFLLAGVGGQGTLLASDIVADVGMRVGADVKKSEVHGMSQ
                     RGGAVTSHVRWGERVASPLCEKGAVDYFIAQEMLEALRWIEFVRSGGTVFLSREQIVP
                     TSTVYGADQYPADQHIVSVVKQVAGKVLLIDARKVAEELGNPRVANSVLLGALSAQID
                     TPAEDWLAVIESRVPQKHVETNRRAFYAGREGGWEI"
     gene            complement(2480..4276)
                     /gene="iorA"
                     /locus_tag="AC162G_13425"
     CDS             complement(2480..4276)
                     /gene="iorA"
                     /locus_tag="AC162G_13425"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HIR5076853.1"
                     /translation="MRQLLSGNEAIARGAWEAGVEVAAAYPGTPSTEILENVARYSEI
                     YSEWSTNEKVALDVTIGAAYAGKRAMAAMKHVGLNVASESLFYSSYTGVNAGLMIISA
                     DDPGLHSSQNEQDNRHYAKFAKVPMLEPADSQEAKDMVAAALDLSEQFDTPVLLRTTT
                     RTSHAKSIVELGERKAAPKRPYQKNPQKYVMIPGFAKRRHPIVEERMVKLAEFAETYA
                     FNRVEIGDSRLGIVATGPSYQYAREVFPTASFLKLGMVWPLPKDLIADFASRVKRLIV
                     VEELDPFFEENIRILGIPVEGKTIFPMVDEFSPEVVRKAAYAAGLIDKPPVEHALDKG
                     ALPMRPPVLCAGCGHRDVFWVLRKLKLTVNSDIGCYALGVMPPLATTDTIGCMGASIG
                     VAAGMRRGGLDGANVAVIGDSTFFHAGMPALANAIYNETPFTTIVVDNRTTGMTGHQG
                     NPGSGMTLHREMHPRIDIEAVVRGMGVEDVTTVDSRDLDDLEEAIKRAVKRNRPSVVI
                     AKTPCVFVTSHAREAYTVVEEDCNGCTLCFRIGCPAIYKSSVMDEKHNRPKAWIDPQA
                     CVGCGLCFDVCARQAILEGAPKMEAALTTHVA"
     gene            complement(4371..4730)
                     /locus_tag="AC162G_13430"
     CDS             complement(4371..4730)
                     /locus_tag="AC162G_13430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5076854.1"
                     /translation="MAFRFESLQIWHMGRDYAKMAYQLTAKFPRKEDYGLTSQINRAA
                     NSISLNIAEGSAKKSAKHFDVYLDNAVGSTFEVVAGGFRALDEGYIDQATLNALYTEG
                     ETLAKAINSFRRTLKLD"
     gene            complement(4801..5532)
                     /locus_tag="AC162G_13435"
     CDS             complement(4801..5532)
                     /locus_tag="AC162G_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076855.1"
                     /translation="MLEVREVSAYYGRIRALDCVSLTVVPGEIISLIGANGAGKTTLL
                     NAISGVVPAAGGEVNWRGQSITRLSPEHIVRLGISHVPERRQVFGSMTVHENLTLGAY
                     TRVRKDGKKAIQRDMDYVFKLFPVLRERIGQLAGTLSGGEQQMLAVGRGLMARPQLML
                     LDEPSLGLAPLLVQEIFRIIVELRRSGTTVLLVEQNARAALRVADRGYVLENGHVVMQ
                     GTAAELAENEELQRAYLGRRNHSGR"
     gene            complement(5525..6316)
                     /locus_tag="AC162G_13440"
     CDS             complement(5525..6316)
                     /locus_tag="AC162G_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5076856.1"
                     /translation="MPSSNHVILEARQLQKAFGGVMAVAGVDLAVRCTEIFALIGPNG
                     AGKTTMFNLVSGTHALDRGEIEFDGQPIHGWPPHRIAARGLVRTFQNIQVFNTMTVLE
                     NVMVGCHTRSRTGFLSAALRLPRVAAEESRMRARAMECLEMVGLEARATDLASSLPFG
                     QQRLLEIARALAAEPKMLMLDEPAAGLNRAETEAVDELIKRVRDDGITVLLVEHDMNL
                     VMGIADRVAVLHYGQKIAEGTPAEVQADERVIEAYLGADWAVFSA"
     gene            complement(6467..7741)
                     /locus_tag="AC162G_13445"
     CDS             complement(6467..7741)
                     /locus_tag="AC162G_13445"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR5076857.1"
                     /translation="MYQPELESLSRTELEALQIDRLRATLRRICAGNPHYAQRLNGLG
                     PDDVHSLDDIRRFPFIHKEDLRQNYPFGLTCAPQKDFVRMHMSSGTTGTPIICPHTAN
                     DVRQWSEIMARCLTAAGLTREDVIQITPSFGLFNGGFGFHYGASAIGAMIVPIGAGRT
                     PLQLQFMRDLGSTALAAIASYPLRLIEVAAQEGFDFRRDARLRVGIFGAEIWSDEIRR
                     RIEERMGIETFDIIGMTETAGVGMGIDCAEHGGLHVWEDHYLIEIVDLESGEPVPDGE
                     SGEMVVTTLTREGLPLIRFRTHDLTRIVSCEPCRCGRTSLRVDRLSGRTDDMIKVKGI
                     NFYPKQVESVLLRHPEVGNDYLIVIDRLEGADRMTIDVEARGLPWPEELSRQLSDELF
                     SMLGLRAEVSILHPGELPRPEGKAVRVQDRRK"
     gene            complement(7728..8720)
                     /locus_tag="AC162G_13450"
     CDS             complement(7728..8720)
                     /locus_tag="AC162G_13450"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5076858.1"
                     /translation="MRLPARRLAIVALVAALAVIPQTVGNNYQIFVLTLIGLYTMLTV
                     GLSLVMGYAGQVSLGHATFYGIGAYTTALLSTNANLPPWLALVVAVIVTALIGFLIGV
                     PIFRLRGHYLAMATLGLNVIFILIVKNESSITGGPSGFTNIPPLSIAGVTFDTDVSMY
                     YVVWIITLIILLLSLNIVNSRVGRALRAIHASEIAAETLGVDSTAFKLKVFALAAGYA
                     GVAGGLYAQTVRFISPSSFDLLFSIELVTMAAVGGLASVWGSIFGAATVVFLTQFIRD
                     RLGDLLAGAAGEGEIIIFGMLLILIMVFLPEGLTTGGLNAVRRWQARRKENVPA"
     gene            complement(8784..9806)
                     /locus_tag="AC162G_13455"
     CDS             complement(8784..9806)
                     /locus_tag="AC162G_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5076859.1"
                     /translation="MIPLSRLCIAADGRGDDQVFTFHGTRITDDMTFVDQLAQFVVSG
                     ITQGSIYALIALGFVTIYSVTGVINFAQGEFAVIGALVAITVSQSASLFRGQVTIELE
                     LPLALAALAGIAASVLAGALVYRLGIQPARHASVLSQIVVTIGASIVLRGLMLLAWGT
                     EPYRLPPFTPGKPIELFGAIVIRQSIWVMGLTLVTLMGLYLFFQHTLIGKALRASAAN
                     PGAARLMGINTLRMGLIAFALSAALGAVAGIVIAPVTFVTYDRGLMLSLKGFVAMIIG
                     GLTSPVGAVIGGLALGVVESLAAGLISSGYRDAFTFGVLFAVLLVRLGGVVRRGKRQA
                     LEGAGL"
     gene            complement(9852..11153)
                     /locus_tag="AC162G_13460"
     CDS             complement(9852..11153)
                     /locus_tag="AC162G_13460"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5076860.1"
                     /translation="MKSRLTLFLTGLALAGMVLSACGGPATTVAPTQPPPPTAPPATQ
                     PPSPTEAPTEEPVTEGDPYVVGAFFSTTGPVSSLGQPEANTVTMLVEQINAAGGIMGP
                     DGLMHTLEVKMYDDQSDATTAVEVAKKLIDEDKVPVIVGGSGSPASIAVNETVSTASI
                     PFISVASSSAIVTKEDGSQHEWVFKTPQQNYPVAQVQGDWLQANGVTKVASLGVNNAF
                     GADSMAALTRVAEELGIEIVYESTFEPGDTDFSAQLTQIAGSDAEAVIIHATPGEGAP
                     LTVQYRDLGLEMPILHNHGIGNRAFIDTAGEAAEGVLFPIGKLLVADQLPDDDPQKEI
                     LLQYVSDYQAAYGTPPSTFGGHAWDGLMMVKAALETVGPDPAAIRDYLENEIKNFVGI
                     SGVFNISEDDHTGIAKESLVLVQIKDGTWAFVDPADYASVP"
     gene            complement(11371..12663)
                     /locus_tag="AC162G_13465"
     CDS             complement(11371..12663)
                     /locus_tag="AC162G_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HIR5076861.1"
                     /translation="MDDLKGTDLIYDWNVMGDVPHYPKHVKVVDETLRDGLQSPSVTH
                     PSIQDKVYLLYLMRCLEIDAADLGLCGAGERFKKDVVVLAREIVNQNMPIVPQSAART
                     LESDIAPIVDASQEAGIAIEACVFLGSSPIRQYAEGWDLSTLLRLTESSVTFAVQRGL
                     PVMFVTEDTTRAQPDALRQIYACAIRSGAHRICAADTVGHATPEGVRNLIRFLRGVVD
                     EVDPAVGIDWHGHKDRGLDIVNTLAAIEAGADRVHGCALGVGERSGNTPMEILLVNLN
                     LLGMANRDLKGLPEYCQVVSQACGVPVPFNYPIVGADSFRTATGVHAAAIAKALDRRD
                     EWLSDRVYCGVPASMVGRTQGIEVGPMSGEHNVRFWLRNHDIEEHPVYIEKILAAAKR
                     SSKLLTDEEIRRMVIVMRQRLKGGEEVTDESLEAALAH"
     gene            complement(12691..13314)
                     /locus_tag="AC162G_13470"
     CDS             complement(12691..13314)
                     /locus_tag="AC162G_13470"
                     /inference="COORDINATES: protein motif:HMM:NF013707.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HIR5076862.1"
                     /translation="MAERIYNLLFVGVGGQGVLLVAEISALAAARAGLDVKQTEVHGV
                     SQRGGSVETHVRFGPVVHSPIVTPGQADVVVALENLEALRFAHFARRETGVVLMNDHE
                     IIPGSVAEAETRYPHNVPSTLQGQGFRVATLKASEMARDLGDGRMANIVMLGALTAYL
                     PFSVDLWLETLQARIPQKFRAANLKAFEAGRAACAPPSGVALAQPAV"
     gene            complement(13377..15134)
                     /locus_tag="AC162G_13475"
     CDS             complement(13377..15134)
                     /locus_tag="AC162G_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5076863.1"
                     /translation="MKELLLANQAIARGAWEAGVTFAAGYPGTPSTEILETLIHYPGV
                     HAQWSTNEKVAFEEGMGAAIGGLRVLVTMKHVGLNVAADAFMVFPYSGTNGGFVVVSA
                     DDPGMHSSQNEQDNRYLALMAKVPIVEPTTAQECKDFVGVALSLSEEFSVPVMFRTTT
                     RLAHHKGLVELGERVEAPRRAFTPDAKRFSVPIYRKLLRPQVEKRLQRLVEYAETSPL
                     NRVEHAGSEIGVVTSGICYQYVKEVLPEAGVLRLGMTHPLPPQLLRDFCARYKMVYVV
                     EEGEAFVESFMRSIGITNLVGKDLFGNIGEYSPGRLAAALGQGDVPQPLGDGITVLPR
                     PPMFCIGCGHRTVFSALRQLKVTVAGDIGCYTMGALPPYEAEHTTFCMGASIGTAYGL
                     EKAGHPRTVAIIGDSTFIHAGIPSLIDAVYNGSSLTLIVLDNSTTGMTGGQPHAGSGQ
                     TLAGGQAPKLDIPAVIKAIGVEKVETVDTWNRKEVGRAIQRALAHKGPSVLIAVGACQ
                     QLPEMKHRELIPYFVDESLCTQCEACFKVWCPAITRTALNFPVISPDECTACTVCAQV
                     CPVDAIYLWKAEAESPLQV"
     gene            complement(15232..16104)
                     /locus_tag="AC162G_13480"
     CDS             complement(15232..16104)
                     /locus_tag="AC162G_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5076864.1"
                     /translation="MLIDFHIHVSRPEHELPWVLEFMAQQYQDGDLWALADQILTPAG
                     LRPFLQENGIDWAVALSEANPITTGVTTNEWVADLCAGANALPDPATGPRGRLIPFAS
                     INPFMVNDLGAELERLVKQFGFRGFKVYPVYQHHYVNDIRMYPLYAAAQELGVPMLVH
                     TGSSVFKGARIKYGDPLHLDDVAIDFPNLTILMAHSGRPFWYEQAFWMARQHENVYLE
                     VSGLPGKKLLEYFPDLERLADKVVYGSDWPGNPYLRKNVEAIEALPLRDETKEKILWK
                     NAAGILGVEVRGGE"
     gene            complement(16241..17296)
                     /locus_tag="AC162G_13485"
     CDS             complement(16241..17296)
                     /locus_tag="AC162G_13485"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR5076865.1"
                     /translation="MKAAIFHGAHQPLTIEDIPTPEPKAGEILVKVAGCGVCHTDLHY
                     IDHDVPTFKKPPLVLGHETSGTVAAVGPPLSPPPSATGGERGGWKEGDRLLLPTVYGC
                     GHCRMCRTGRENICENMVMFGSYVDGAYAEYIISPAKDAIPLPPEIPLVEGAIIADAT
                     TTPFHAVVNRGRVQPGDAVVVIGCGGIGLNCVQIAAAVGGQVIAVDIADHKLEWARKL
                     GAKATVNPKAVERLDKELRKLTGGGADIAFECIGNPITQEQAFNSVHAGGRFVVVGSS
                     DKPMTLNTGKVMFREIEIIGSLGCRAVDYPRVLELARQGKIKVAELVTAKFPLDQIND
                     ALDTLRRGEGIRSVIVP"
     gene            complement(17329..17793)
                     /locus_tag="AC162G_13490"
     CDS             complement(17329..17793)
                     /locus_tag="AC162G_13490"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR5076866.1"
                     /translation="MHEPFKFLTPVRVRFNETDLQGHVNFGHYLFYFDIGVTDYLRAI
                     GYSYERMSAEGIDMVYGESRARYLSPAYFDETLNIHTRIGHIGNSSMRFDFQVIEASR
                     GRRVAEGEITVVMIERDTRKKMRVPEPLREAVARYEEVKPVVTTSGVRQDSD"
     gene            complement(17868..19547)
                     /locus_tag="AC162G_13495"
     CDS             complement(17868..19547)
                     /locus_tag="AC162G_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR5076867.1"
                     /translation="MTRKFDPVTLEILWRRLLSIVDEADAAVARTAFSSLLRDAHDYT
                     CMFTDRQGRELAQGTFATPGQSGAMALGIKKLVRQLPVESFRPGDVYITNDPWALAGH
                     LNDVCVISPVFYHDCLTAFTACVLHHSDIGGRVASDNHDVFEEGLFIPLVKLYDGGVL
                     NEAVLEMIRWNVRTPEQVIGDIRSQIASNHVCAEQVVRMLAEYHLDGLDDLADEIIAR
                     SEQSIRASIAKVAPGIYRAEGVIEQMEGRADLVIQCAVEVAGSDITVDLAGSSPQVDW
                     GGNVVYNFTYAYVHMAIKSIFDPEIPNNDGSAAPIHLKAPEGTVVNCRFPAAVAARMQ
                     IGHFMTEIIYRALAQALPNRVIVGSGGTPATMQVFYGWRNNGQPFHAVLIRGGGMGAG
                     GLRDGEGCFIFPANGANTPVEILESDTPLIVERRELLPDSGGPGQQRGGLGRREVFCV
                     PNDEHAPQPPVNLGIQSGRFRLPPEGLFGGLPGARARFLVNGQPGNPYSLTRLRPGDV
                     VIMDAAGGGGYGDPKERDREALLCDVRDGKVSRESALRDYGVDITDQTLKE"
     gene            complement(19544..21652)
                     /locus_tag="AC162G_13500"
     CDS             complement(19544..21652)
                     /locus_tag="AC162G_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR5076868.1"
                     /translation="MRLGIDIGGTFTDFVLLDDATGQITSYKCLTTPRDPSDAIEEGI
                     RGLADKVSDYVNDLEEIIHGTTLVINSIIERKGARTGLITTQGFRDVLELGREIRYAP
                     YDVFAEFPEPLVPRRWRLEVNERVRADGSVLKLLDAEDARARVRSLGSMGVESVAVCL
                     LNSFENPTHEIALKRIIAEELPSTSISISYEVLPQIREYERTSTTVANAYVKPLTEKY
                     LRRLYDRLAGLGFHGRLFIMLSSGGVTSAETAAEFPVRIIESGPTAAVIAGEYFSRLF
                     KSPEMFCFDMGGTTAKSCLIQNGVAGVVPTFEVGRVQRFMKGSGLTIQVPVVDLMEIG
                     AGGGSIARMSRRGTLQVGPESAGAEPGPICYGRGGAEPTVTDADLLLGYLDADYFLGG
                     AMKLDVEAARRGIEEKVAKPLGVSYLQAVWGIHDLINETMTAAAKTHIAERGGNPKIV
                     TLAAFGGAGPVHAYGLAQKLGAPRVVVPPNAGVGSALGFFTAPRAFDLVRSHKAPVFS
                     ADFAAIERLFREMESEGERTLQTSRVSETREVSIHFSRSVDARFIGQGSETNLPIPEH
                     DFTTLDPVEVRRRFDEVYTRLYGRTYPESPVEFVNFRVRACLPVRLLELPRLPNRGGH
                     IQDAIKGERKAFSGLARDFIPFTVYDRYELFPGATFSGPAIVEERESTVVVGEDGMAS
                     VDEFGFLWVELPDPSGLRMP"
     gene            complement(21710..22888)
                     /locus_tag="AC162G_13505"
     CDS             complement(21710..22888)
                     /locus_tag="AC162G_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5076869.1"
                     /translation="MSLFSDLTVLSLEQATTLPFLTQRLAREGVRVIRLEPPGRGDPN
                     RYVGRDVLGEPGMASYFFANNCGKQAITLNLADPEGRAILHELIAKLPVDIFATNNRP
                     SSYAKLGVDYETIKTIRPETIWLGITGFGPNCDEAAYDPVLQARAGWMELTGDPDGPP
                     LVFGLPMVDLGAAEHAYGALMKALYRRAVTGEGSRIDISMYHSALAWMAGPIMLTALG
                     ERVTRRGNTHQFFAPVSVYPTRDGYVYIAVGNDRQWEAITQLPGFEGLSSPGYQRNAG
                     RIADVERLNQRMSDCTRAMMSDDLITALNRIGVPVARVNTLNDVLSEPLARDSLLRVR
                     DDRTATELVLAPVAEVNDGAAATLSFPPRLGEHNETILGEVLGYGREQLSELKERGVL
                     "
BASE COUNT         4240 a         7644 c         6867 g         4326 t
ORIGIN      
        1 catcacggcg atgcccgatg ccagcccgcg ccgccgggcg gcaaaccagg ccgacagcaa
       61 tcccatcacc gggacgttgc tcaacccgct gccgacgccg gtcaacgcgc gccagatggc
      121 cgccggcagg aacgcttgcg ccaggccggt gagcgccatc cctaaaccgg ccagaaccaa
      181 cccgactgtg acaattcgcc gtgggccgaa gcgcgcggcc aacgccccgc cgatgatcga
      241 gaacgccagg tatccgacga ggttcgccgt cgccagcccg cccgcctgcg cgttgctcag
      301 ctccaggctc gcctgcatgg acggcaggat gacgctgtag ccgaaccgcg ccaggccgag
      361 cgagccgaac acgaccggca cgctcaccgc caggataacc cagccgtagt ggatgcggcg
      421 ggcaggcgct gtcgttgccg cgctcactac agctctcgct tatcgatcac gcgcttcgcc
      481 ttgccttcgt agcgctggat cgacctcggc cccatcagtc tcacgacggc cgagatgccg
      541 agcgtctgcg ccagttcagc agatacgcgg ttctgaagct cgtccagccg ccgcgtgtcg
      601 atgggctgga acagcttcgg cgaggcctcc acccacactt cgagcgtatc gagctggtcc
      661 tttgggcggt cgagcacgat ctggtagtgc ggctcgatgt cctcgaccga gagcagcgtc
      721 ttctcgatca gttgcgggta gacgttgacg ccgcgcacga taagcatgtc gtcggtgcgc
      781 ccgagcacct tgtccatgcg cacggtcgtc cgcccgcaag cgcacttgtc cgcgtgcagg
      841 cgcgtgcggt cgcgcgtgcg gtagcggatc accggcatgg cttccttggt cagcgtggtg
      901 aagaccaatt cgccttcttc ctcgtagggc agttgctcgc cggtttccgg atcgatgatc
      961 tccgccagga aatgatcttc ctggatgtgc atgccgcagt tatgctcgca ctcgatcgat
     1021 acgccagggc caatgatctc ggtgaggccg tagatgtcgt aggccttcag gccgagcttg
     1081 gactcgatct cggtacgcat cttgtcgctc cacggctctg cgccgaagat gcctacccga
     1141 accttcatgc gtttcttgaa gtccaggccc atctcggcgg cttcttcgcc gatgacgagc
     1201 gcgtacgacg gtgtgcaggt cagcaccgtc gcggcgaaat cctccatcag catcacctgc
     1261 cggcgcgtca acccgcccga gatgggcacc accatggcgc cgactttctc cgcgccgagg
     1321 tgaaagccta acccgccggt gaacaggccg tagccgtatg cgttatgaac gacatccttg
     1381 ctcgtcacgc cggcgcacgt caccgtgcgg gccattacct ccgtccacaa gtcgatgtcg
     1441 gcgcgcgtgt agccgccgac cgtcggcttg ccggtcgtgc cggacgaggc gtggatacgg
     1501 acgacctgat ccaggggcac agtgaacatg ccgaaggggt agttgtcgcg cagatcggcc
     1561 ttttcggtga aaggcgggcg cttcaggtcg tcgagcgatt gaagatcgtc ggcgctcacc
     1621 ttcgcctcgg cgaacttctt cttatagaac ggcacgcttg cagccgcgcg ggcgacgacg
     1681 tcccgcagcc gccgcgcctg aaccttgttc agctggtcgc gcggcatcga ctcgaattca
     1741 ggattccaga taggcatggc ttctcctttc gcgtatcaaa tctcccaccc gccctcgcgc
     1801 ccggcataaa acgcccggcg gttggtttcg acgtgtttct gcggcacccg ggactcgatc
     1861 acggccagcc agtcctcggc gggcgtgtcg atctgggccg agagcgcgcc gagcagcacg
     1921 ctgttggcga cgcgcgggtt gcccagctcc tcggccacct tgcgcgcgtc gatcagcaga
     1981 actttgccgg caacctgctt gacgaccgaa acgatgtgct ggtcggccgg atactgatcg
     2041 gcgccgtaca cggtcgaagt cggaacgatc tgctcgcggc tcaggaacac cgtgccgccc
     2101 gaacgcacga actcgatcca gcgcagcgcc tcgagcatct cctgggcgat gaagtagtcc
     2161 accgcgcctt tctcgcacag cggtgaagcg acgcgctcgc cccagcgcac atgactggtg
     2221 accgccccgc cccgctgcga catgccgtgc acctcggact tcttgacgtc tgcgccgacg
     2281 cgcatgccca cgtcggctac aatgtcgctg gccagcaagg ttccctgtcc gcccacgccg
     2341 gcgagaagaa agtttgccgt taccatgttg cctccctgct cagtcgtcag ttatcagtct
     2401 tcagttatca gtgatcagtt atcggtcgtc agtaatcact gttcactgct tactgatcac
     2461 tgttcactga tcactggcct tacgccacgt gcgtcgtcag cgccgcttcc atcttcggcg
     2521 cgccttctag aatcgcctgc cgcgcgcaca cgtcgaagca caggccgcag cccacgcacg
     2581 cctgcgggtc gatccatgcc ttcggccggt tgtgtttttc gtccatcacg ctgctcttgt
     2641 aaatcgccgg gcagccgatg cggaagcaca acgtgcagcc gttgcagtcc tcctctacga
     2701 cggtgtacgc ttcgcgcgcg tgcgatgtga cgaacacgca cggcgtcttg gcgatgacca
     2761 ccgacggccg gttacgcttg acggcccgct tgatggcctc ctcgaggtcg tcgaggtccc
     2821 gtgagtcgac ggtcgtcacg tcctcgacac ccatgccgcg cacgacggct tcgatgtcga
     2881 tgcgggggtg catctcgcgg tgcagcgtca tgcccgatcc gggattgccc tggtggccgg
     2941 tcatgccggt cgtgcggttg tcgacgacga tcgtcgtgaa cggcgtctcg ttgtagatgg
     3001 cgttcgccag cgcgggcatg ccggcgtgga agaacgtgct gtcgccgatg acggccacgt
     3061 tcgcgccgtc aagcccgccg cgccgcatgc ccgccgccac accgatgctc gcgcccatgc
     3121 agccgatcgt gtcggtcgtg gccagcggag gcatcacgcc tagcgcgtag cagccgatat
     3181 cgctgttgac ggtgagcttc agcttgcgca ggacccagaa cacgtcgcga tggccgcagc
     3241 cggcgcacag caccggcggg cgcatgggca gtgcgccctt gtcgagcgcg tgctcgaccg
     3301 gcggtttgtc gattagcccg gcggcgtagg ccgccttgcg caccacctcc ggcgagaact
     3361 cgtcgaccat cgggaaaatg gtcttaccct cgaccgggat gcccaggatg cggatgtttt
     3421 cttcgaagaa cggatcgagc tcctcgacga cgatcagccg cttcacccgg gaagcgaagt
     3481 cggcaatcag gtccttcggc agcggccata ccatacctag cttcaggaac gacgcagtcg
     3541 ggaacacttc gcgagcgtac tggtaactcg gcccggtagc gacaatcccc aatcgcgagt
     3601 ctccaatctc aacccggttg aacgcgtatg tctctgcgaa ctcggctaat ttgaccatgc
     3661 gctcttcgac gatgggatgg cggcgcttgg cgaagccagg gatcatcacg tacttctgag
     3721 gattcttctg atagggccgt ttcggcgccg ccttccgctc gcccagttcc acaatggatt
     3781 tggcgtgcga ggtgcgcgtc gtcgtgcgca gcagaaccgg cgtatcgaac tgctcgctca
     3841 gatcgagcgc cgcggcgacc atgtccttcg cctcctgact gtcggcaggc tcgagcatcg
     3901 gcaccttggc gaacttggcg tagtgacggt tgtcttgctc gttctgcgac gagtgcagtc
     3961 cgggatcgtc ggcgctgatg atcatcagcc cggcgttgac gccggtgtat gacgagtaga
     4021 acagcgactc ggacgccacg ttcaggccga catgcttcat cgcggccatg gcgcgcttgc
     4081 cggcgtacgc cgcgccgatc gtgacgtcga gcgcgacctt ctcattggtt gaccactccg
     4141 agtagatctc cgagtagcgc gccacgttct ccaggatttc ggtgctcggc gtcccgggat
     4201 aggctgccgc cacctcgaca cccgcctccc acgcgccgcg tgcaatcgct tcgttgcccg
     4261 acagtagttg cctcatcgca gcctccttct tgctgatggc tgatagccga tagctgatag
     4321 ctgatggctg atggccaata gctaatggcc gatagccgat agtagacgac tcaatccagc
     4381 ttcaacgttc gacggaacga gttaatagcc tttgcaagag tctcgccctc ggtatacaag
     4441 gcattgagcg tggcttgatc aatgtaaccc tcatccaaag cccggaaacc gcccgcgaca
     4501 acctcgaagg tcgagccaac cgcgttgtcc agatatacgt cgaagtgctt ggcactcttc
     4561 ttggctgatc cttcagcaat attcagcgaa atcgagttgg ccgcccggtt tatctgagag
     4621 gtaagaccgt aatcctcttt gcgtggaaac ttagccgtca gctgataggc catcttggca
     4681 tagtcacgcc ccatatgcca tatctgcaaa ctctcaaacc tgaacgccat aaacctctcc
     4741 cccactttca gctatctgcc atccgctatc tgctatcggc tatctgctat ccgccatccg
     4801 ctatctgcca ctgtgatttc gccgccccag atacgcgcgc tgcagttcct cgttttccgc
     4861 aagctcggcg gccgtgccct gcatcaccac gtggccgttc tccagcacgt agccccgatc
     4921 ggccacgcgc agcgcggcgc gggcgttttg ctcgacgagc aagaccgtcg tcccgctccg
     4981 gcgcagctcg acaatgattc ggaatatctc ttgcaccagc aatggcgcca gccccagcga
     5041 cggttcatcc agcagcatca gttgcgggcg cgccatcaag ccgcgcccga cggccagcat
     5101 ttgctgttcg ccgccggaga gcgtgccggc gagctggcca atccgctccc tgagcaccgg
     5161 gaacaacttg aacacgtagt ccatatcccg ctgaatcgcc ttctttccgt ccttccgcac
     5221 ccgggtgtag gcgccgagcg tcaagttctc gtgcaccgtc atcgatccga agacctgccg
     5281 tcgctcgggc acgtgactga tgcccaggcg gacgatgtgt tcgggcgaga ggcgggtgat
     5341 agattggcct cgccagttga cttcgccgcc tgccgccggg accaccccgg aaatggcatt
     5401 gagcagggtc gtcttgcccg cgccattcgc gccaatgaga ctgatgatct cgcccgggac
     5461 caccgtcagc gacacgcaat cgagcgcgcg gatgcggccg tagtaggcgc tgacctcgcg
     5521 cacttcaagc actgaacacc gcccagtctg cgccgaggta cgcctcgatc acgcgctcgt
     5581 cggcctgcac ttcggccggc gtgccttcgg cgatcttttg cccataatga agaacggcca
     5641 cgcgatcggc gatgcccatg accagattca tgtcgtgctc gacgagcagc acggtgattc
     5701 cgtcatcgcg gacgcgcttg ataagctcgt cgaccgcttc ggtctcagcg cggttgaggc
     5761 cggccgctgg ctcatcgagc atcagcattt tcggctccgc cgccagcgcg cgcgcgatct
     5821 cgagcaggcg ctgttgtccg aacggcaggc tggacgccag atccgtcgcc cgcgcttcca
     5881 ggccgaccat ctccaggcac tccatcgcac gcgcccgcat gcggctctcc tcggcggcga
     5941 cgcgcggcag gcgcaaggcg gctgacagga atcctgtccg gctgcgggtg tggcacccca
     6001 ccatcacgtt ctcgagcacg gtcatggtat tgaagacctg aatgttttga aaggtgcgca
     6061 ccagccctcg cgcggcgatg cggtgtggcg gccagccgtg aatcggctgg ccgtcgaact
     6121 cgatttcgcc ccgatcaagc gcgtgcgtgc cggaaaccaa gttgaacatc gtcgtcttgc
     6181 ccgcgccgtt cgggccgatc agcgcgaaaa tctctgtgca ccggacggcc aggtctacac
     6241 cggcgacggc catcacgccg ccgaacgcct tctgcagttg tcgtgcttcg agtatcacgt
     6301 gatttgatga aggcatatca cttatcgctg atggcagcta gccgatggct gatggcgcat
     6361 ggcctatggc ttatcgccac aagcagccga ccgactatcc gatccctcct caccaattac
     6421 cacttaccga ttactgctca ctgattactg ctcactgatt accgccctac tttctccgat
     6481 cctgcaccct gacggccttg ccttccggcc ggggcagctc gccaggatgc aaaatgctga
     6541 cttcggcgcg cagccccaac atgctgaata actcgtccga caactgccgc gacaattcct
     6601 cgggccaggg caacccgcgg gcttccacgt cgatcgtcat ccggtcggcg ccctccaggc
     6661 gatcgatcac gatgaggtag tcgttgccca cttcgggatg cctgagcaag acgctttcca
     6721 cctgcttcgg atagaagttg atgcctttca ccttgatcat gtcatcggtg cgacccgaca
     6781 ggcgatcgac gcgcaggctg gtccgcccgc agcggcatgg ttcgcagctg acgatgcgcg
     6841 tgaggtcgtg cgtgcggaag cgaatgagcg gcagcccttc gcgcgtcagc gtcgtcacga
     6901 ccatttcgcc tgactcgccg tcaggaaccg gctcaccgct ctccagatcg acgatctcga
     6961 tcaaataatg atcttcccac acgtgaaggc cgccgtgctc ggcgcagtcg atccccatgc
     7021 caacgccggc cgtttcggtc atgccgatga tgtcgaacgt ctcgatgccc atccgctcct
     7081 cgatgcggcg gcgtatctca tcgctccaga tctccgcgcc gaagatgccc acgcgcagcc
     7141 gggcgtcgcg gcgaaagtcg aagccttcct gcgcggcgac ctcgatcagg cgcagcggat
     7201 acgaggcgat ggcggccaga gctgtcgagc ccagatcgcg catgaactgc agttgcagcg
     7261 gcgtgcgccc cgccccgatc ggaacgatca tcgcgccgat agcgctcgcg ccgtagtgga
     7321 agccgaagcc gccgttgaac aggccgaacg aaggcgtaat ctgaatcacg tcctcgcgcg
     7381 tgaggccagc cgcagtcagg cagcgcgcca tgatctcgct ccactgcctc acgtcatttg
     7441 ccgtgtgcgg gcagatgatc ggcgtgcccg tcgtccccga gctcatgtgc atgcgcacaa
     7501 aatccttctg cggcgcgcag gtcagcccga acggatagtt ctggcgcagg tcttccttat
     7561 ggataaacgg aaagcgacgg atgtcatcca gactgtgaac gtcgtccggg ccaagcccat
     7621 tcagccgctg cgcgtagtgt ggattgccgg cgcagatgcg gcgcagcgtc gctcgcaggc
     7681 ggtcgatctg tagggcttcc agctcggtac gggaaagcga ctcgagttca ggctggtaca
     7741 ttttccttcc gcctcgcttg ccatctccga accgcattca agccgccggt cgtcagccct
     7801 tccggcagga acaccataat gagaatcaga agcatgccga agataatgat ctcgccctcg
     7861 cccgccgcgc ccgcaagcag atcacccaac cgatcccgaa taaactgcgt gaggaatacg
     7921 accgtcgcag cgccgaagat cgacccccac acgctggcca gcccgccgac ggcggccatc
     7981 gtcacgagct cgattgaaaa cagcaggtcg aagctggatg ggctgatgaa gcggacagtc
     8041 tgcgcgtaca agccgcccgc gacgcccgca tacccggcgg ccagcgcgaa caccttgagc
     8101 ttgaaagccg tggaatcgac gccgagcgtc tccgccgcga tctcactggc gtggatcgcg
     8161 cgcagcgccc ggcccacgcg tgagttgaca atgttgagtg acaacaacaa gatgatcagc
     8221 gtgatgatcc acaccacata atacatcgac acgtcggtat cgaaggtgac gccggcgatc
     8281 gacaggggtg ggatgttggt gaagcccgaa gggcctccgg tgatcgacga ctcgttcttg
     8341 acgatcagga tgaagatcac gttgagtccc agcgtggcca tcgccaggta atggccgcgc
     8401 aggcggaaga tcggcacgcc gatgagaaag ccgatcagcg ccgtcacgat cacggcgacg
     8461 acgagcgcga gccagggggg caggttggca ttcgtgctca gcagcgccgt cgtataggcg
     8521 ccgatgccat agaacgtggc gtggcccagc gacacttgtc cggcgtagcc catcacgagc
     8581 gacaacccca cggtcagcat cgtgtacagg ccgatcagcg tcaggacgaa tatctgatag
     8641 ttgttgccga cggtctgcgg gatgaccgcc agcgctgcca ccagcgcgac gatggccagc
     8701 cgcctagcgg gaagtctcat cttcattgca ccatctcgct ccacgctcca cgactccact
     8761 ctccacgact ccacgctccc caattacaac cccgccccct ccaaagcctg cctctttccg
     8821 cgccgcacca cgccgcccag ccgcacgagc agcacggcga acagcacacc aaacgtgaag
     8881 gcgtctctgt agccggacga gatcaacccc gcggccagcg actccacgac ccccagggcc
     8941 aggccgccaa tcactgctcc gacagggctg gtcaggccgc cgatgatcat tgccacgaac
     9001 cccttcaacg agagcatcaa gccccggtca tacgtcacga acgtcaccgg cgcaatgacg
     9061 atcccggcca ccgcgcccag cgctgcgctg agcgcgaaag cgatcagacc catgcgcagc
     9121 gtgttgatgc ccatcagacg cgcggcgccc gggttggccg cgctcgcccg cagtgccttt
     9181 ccgatcagcg tatgctggaa aaacagatac aggcccatga gggtcaccaa cgtcaagccc
     9241 atcacccaaa tactctgccg gatgacaatc gccccgaata gctcgatcgg cttgccgggc
     9301 gtgaacggcg gcaggcggta aggctcggtt ccccacgcca gcaacatcag cccgcgtagc
     9361 acgatcgacg cgccgatcgt cacgacgatc tggctgagca ccgacgcgtg ccgcgccggt
     9421 tgaatgccca ggcggtacac gagcgccccg gccagcaccg acgccgcgat gccggcgagc
     9481 gcggccagcg ccagcggcag ttccagctca atcgtcacct ggccccggaa gagcgaggcg
     9541 ctctgcgata ctgtgatggc gaccagcgcg ccgatcacgg caaactcgcc ctgggcgaag
     9601 ttgatcacgc cggtcacgct atagatcgtg acgaagccca gcgcgatcag cgcgtagatg
     9661 ctgccttgcg tgataccgga gacgacgaac tgagcaagct gatcgacgaa agtcatatcg
     9721 tccgtaatgc gtgttccgtg aaacgtaaaa acctggtcat cccctctccc gtctgcggcg
     9781 atgcacaggc gggagagggg aatcacgtat cacgcttcac gtttcacgcg tcacgctcca
     9841 cgctccacca atcacggcac acttgcgtag tccgccggat cgacgaacgc ccatgtgcca
     9901 tctttgatct gcaccagcac cagcgattcc ttcgcgattc cggtatgatc gtcctcgctg
     9961 atgttgaaaa caccgctgat cccgacgaag ttcttgatct cgttttcgag atagtcgcgg
    10021 atcgccgccg ggtcaggccc caccgtttcc agcgctgctt tcaccatcat gagcccatcc
    10081 caggcatgcc cgccgaacgt actgggcggc gtgccatacg ccgcctgata gtcggacaca
    10141 tactgcagca ggatctcctt ctgcggatcg tcatccggca gttggtcggc caccagcagc
    10201 ttgccgatcg ggaacagcac gccttcggcg gcttcgccgg ccgtgtcgat gaacgctctg
    10261 ttgccgatgc catggttgtg caggatcggc atctccaggc ccaggtcgcg atactgcacc
    10321 gtgagcggcg cgccctcgcc gggtgtggcg tggataatca ccgcttcggc atcgctgccg
    10381 gcgatttgcg tcagttgcgc cgagaagtcg gtgtcgccag gctcgaacgt gctttcgtac
    10441 acgatctcga tgccgagttc ctccgccacc ctcgtcaacg ccgccatcga gtcggcgccg
    10501 aaggcgttgt tgacgcccag cgacgcgacc ttggtgacgc cgttcgcctg cagccagtcg
    10561 ccctgcacct gggccacggg gtagttctgc tgcggcgtct tgaacaccca ctcgtgctgc
    10621 gacccatcct ccttcgtgac gatcgcggac gacgaggcca ccgagatgaa cgggatgctg
    10681 gcggtgctga cggtctcgtt cacggcgatg ctggccgggc tgcccgaccc gccgacgatc
    10741 accggcacct tgtcttcgtc gatcagcttc ttcgccacct cgacggccgt ggtggcgtcc
    10801 gactgatcgt cgtacatctt cacttccagc gtgtgcatca gcccatccgg ccccatgatg
    10861 ccgcccgccg cattgatctg ctcgacgagc atggtcacgg tgttggcctc gggctggccc
    10921 agagaggaca ccggcccggt cgtcgagaag aacgcgccga cgacgtatgg atccccttct
    10981 gtcaccggtt cttccgtagg cgcttccgtc ggggaaggcg gctgggttgc cggcggcgct
    11041 gtcggcggcg gaggttgcgt cggcgcgacg gtcgtcgcag ggcctccgca ggccgaaagc
    11101 accatcccag ccagcgccag tccggttaga aacaaggtca gtctgctctt catggctctc
    11161 ctcctggttg tgcgtgagtt gtgcgtctga caaaacgact aattcggcaa agcaagtttc
    11221 atgccgcggc agatcacctc cttttcgccg cgccgagacc caatactggc ccgggacggc
    11281 agtgcaacta cggtcccaac aatcggcgtt cgccggctgc gaaatcttgg ctaaccggag
    11341 atcgttagac tctaactcta actcccaact ctaatgtgca agcgccgctt cgagcgactc
    11401 gtccgtgact tcctcgccgc ccttcagtcg ctggcgcatc acgatgacca tgcggcggat
    11461 ctcctcgtcg gtcaggagct tgctcgagcg cttggcggcg gcgaggatct tctcgatgta
    11521 caccggatgc tcttcgatgt cgtggttgcg cagccaaaag cggacgttgt gctcgcccga
    11581 catcggtccg acctcgatgc cttgcgtccg cccgaccatc gaagccggca cgccgcagta
    11641 cacgcggtcg gacagccact cgtcgcgccg gtcgagcgcc ttggcaatcg ccgccgcgtg
    11701 cacaccggtc gcggtgcgaa acgagtccgc gccgacgatg gggtaattga atggaaccgg
    11761 cacaccgcac gcctgcgaca caacctggca atattccggc agccccttca ggtcacggtt
    11821 ggccatgccg agcaggttga gattgaccaa caggatctcc atcggcgtgt tgccggagcg
    11881 ctcgccgacg cccagcgcgc acccatgcac ccggtccgcg ccggcctcga tcgccgcaag
    11941 cgtattgacg atgtctagtc cacgatcttt gtggccgtgc cagtcgatgc ccaccgccgg
    12001 gtcgacctcg tccaccacgc cgcgtaggaa tcggatcagg ttgcgcacgc cttcgggagt
    12061 ggcatggccg accgtgtcgg cggcgcagat gcggtgggcg cctgagcgga tggcgcaggc
    12121 atagatctgg cgaagcgcat ccggctgggc gcgcgtcgtg tcctcggtga cgaacatcac
    12181 cggcagcccg cgttggacgg caaacgtcac cgacgactcg gtcagccgca gcagcgtgct
    12241 cagatcccag ccttcggcgt attgacgaat gggactggag ccgaggaaca cgcatgcctc
    12301 gatggcaatg cccgcttcct gcgaggcgtc cacgatcggg gcgatgtcgc tttccagtgt
    12361 gcgagccgcg ctctgtggca cgatgggcat attctggttg acgatctcgc gcgcgagcac
    12421 gaccacgtct ttcttgaacc gctcgcctgc gccgcacagc cccagatctg ctgcatcgat
    12481 ctccaggcaa cgcatgaggt acagcaagta caccttgtcc tggatcgaag ggtgcgtgac
    12541 ggacggtgac tgcaggccgt cacgcagcgt ctcgtcgaca actttgacgt gcttcgggta
    12601 atgcggcaca tcacccatca cgttccagtc gtaaatcaag tcagttccct tgaggtcgtc
    12661 catggtttcc tcctcatcag ccgctgcgcg tcacacggca ggctgtgcca gcgcgacccc
    12721 gctgggcggg gcgcaagcag cgcgcccggc ttcaaatgct ttcaagttgg cggctcgaaa
    12781 cttctgcggg atgcgcgctt gcaacgtctc cagccacaag tcgacgctga agggcagata
    12841 cgcggtcagc gccccgagca tgacgatatt cgccatccgc ccatctccca gatcgcgcgc
    12901 catctcggac gccttcaaag tcgccacacg aaagccctga ccttgcaagg tcgacggtac
    12961 attgtgtgga tagcgcgttt cggcttcggc aaccgatccg gggatgattt cgtgatcgtt
    13021 catcaacacc acaccggtct cgcgacgagc gaagtgcgcg aagcgcagcg cctccaggtt
    13081 ctccagcgcg acgaccacgt cggcctggcc gggcgtgacg atgggcgagt gcacgaccgg
    13141 cccgaatcgc acgtgcgttt cgacgctccc gccgcgctga gaaacgccat gaacctcagt
    13201 ctgcttgaca tccagcccgg cgcgcgcggc cgcgagcgcg ctgatctcag cgaccagcag
    13261 cacgccctgg ccgccgaccc ccacgaacaa gaggttatag attcgttccg ccatatctct
    13321 ccttcaaccc cccaccccac tgaggcgctc tgcagagtga agccggtaag cgccgcctac
    13381 acctgaagcg gcgactccgc ctcggccttc cacagataaa tcgcgtcgac cgggcacacc
    13441 tgagcacaca ccgtgcaggc tgtgcactca tcgggactga tcaccggaaa gttcagcgcc
    13501 gtacgtgtga tggcgggaca ccataccttg aagcacgcct cgcactgcgt gcacagcgac
    13561 tcgtcgacga aatacggtat caactcccgg tgcttcatct ccggcagttg ttggcacgcc
    13621 ccgaccgcga tcaagaccga cggccccttg tgcgcgagcg cccgctggat agcgcgcccg
    13681 acttccttgc ggttccaggt atcgacggtc tccaccttct cgacgccgat cgccttgatc
    13741 acggcaggga tgtcgagctt gggtgcctgg ccgccggcga gtgtctggcc cgaccccgca
    13801 tgcggctgcc cgccggtcat gcccgtcgtc gagttgtcga gcacaatcag cgtcagcgag
    13861 ctgccgttgt acacagcgtc gatcagcgac gggattccgg cgtggatgaa cgtcgagtcg
    13921 ccgatgatag ctacggtgcg cgggtgaccg gctttctcca acccgtacgc cgtgccaatg
    13981 ctggcgccca tgcaaaaggt ggtgtgctcg gcttcgtagg gcggcagcgc acccatcgtg
    14041 taacagccga tgtcgccggc aaccgtcacc ttgagttggc gcagcgcact gaatacggtt
    14101 cggtggccgc agcctatgca gaacatcggt gggcgcggca gcaccgtgat gccgtcgccc
    14161 agcggctgcg gcacgtcgcc ttgccccaac gcagcggcca gtcggcctgg agagtactcg
    14221 ccgatgttgc cgaacaggtc cttgccgacg aggttggtga tgccgatcga gcgcataaag
    14281 gattcgacga aggcttcgcc ctcttcgacc acatatacca tcttgtagcg cgcgcagaag
    14341 tctctcagca gttgcggcgg cagcgggtgc gtcatcccca gtcgcagtac tcccgcttcc
    14401 ggcagcactt ccttcacgta ctgatagcag atcccgcttg tcactacgcc gatttcgctg
    14461 ccggcatgtt ccacgcgatt gagcgggctg gtctcggcat actcgaccag gcgctgcagg
    14521 cgcttctcca cctgcgggcg gagcagcttg cggtagatcg gcacggagaa gcgctttgcg
    14581 tcgggtgtga acgcgcggcg cggcgcttcg acgcgctcgc ctagctccac cagtcctttg
    14641 tggtgcgcga gccgggttgt cgtgcggaac atcaccggca cactgaactc ttccgaaaga
    14701 ctcagcgcga cgccgacgaa atccttgcac tcttgtgcgg tggtcggctc gacgatgggc
    14761 accttggcca tcagcgccag gtagcggtta tcttgctcgt tctgcgagga gtgcatgccg
    14821 ggatcgtcag ccgagacgac gacgaagccg ccgttcgtcc cggagtatgg gaagaccatg
    14881 aaagcgtcgg ccgccacgtt cagcccgacg tgcttcatcg tcaccagcac gcgcagcccg
    14941 ccgatggccg cgcccatgcc ctcttcaaag gcgaccttct cgttggtcga ccactgggca
    15001 tgcacgcccg ggtagtggat aagtgtttcc agaatctcgg tgctcggcgt gccggggtaa
    15061 ccggcggcga atgtcacacc ggcttcccac gcgccccgcg caatcgcctg attggcgagc
    15121 aagagttcct tcatcgtgtc cacctttccg cttattgctt atcgcctatg gctcatcgcc
    15181 tatcggttgc caagctcgat aagcgacagg cgatatgcca tctgctatct gctattcgcc
    15241 gcctcgcact tccacgccca aaattcctgc cgcgttcttc cacaaaatct tctccttcgt
    15301 ctcgtctctc aaaggcagcg cttcaatcgc ttcgacgttc tttcgcaggt aaggattgcc
    15361 tggccagtcc gagccgtaga ccaccttgtc cgccaggcgc tccaggtccg ggaagtactc
    15421 cagcagtttc ttgccgggca gaccggagac ttccaggtag acgttttcgt gctgccgcgc
    15481 catccagaac gcttgctcgt accagaaggg ccggccggaa tgcgccatca ggatcgtaag
    15541 attgggaaaa tcgatggcca cgtcgtcgag atgcagggga tcaccgtatt tgatgcgcgc
    15601 gcccttgaac accgacgagc cggtgtgcac cagcatcggc acaccgagct cttgcgccgc
    15661 cgcatacaac gggtacatgc gaatgtcgtt cacgtaatga tgctggtaca ccggatatac
    15721 cttgaacccg cgaaagccaa actgcttgac caatcgctcc agctctgcgc ccaggtcgtt
    15781 caccatgaac gggttgatgc tggcgaatgg gatcaggcgc ccgcgggggc cagtcgcggg
    15841 gtcgggcagc gcgttggccc ccgcgcacag atcggctacc cactcgttcg tcgtcacgcc
    15901 cgtcgtaatc ggattggcct cggatagcgc cactgcccag tcgatgccgt tctcctgcaa
    15961 gaatggccga agtcccgccg gcgtcagaat ctggtcggcc agcgcccaca agtcgccgtc
    16021 ctgatactgc tgcgccatga actccagcac ccagggaagc tcgtgctccg gtcgtgagac
    16081 gtgaatgtga aagtcaataa gcaacggcga acctctgagt cagtgatcag taaccagtga
    16141 tcagtcatca gtcatcagcc atcagtcgtc ggtcaccagt caccggtcat cggtcaccac
    16201 cggtcactga tcactgatta ctgattactg ataactgtct ctaaggcacg atgacggagc
    16261 ggatgccctc gccgcgccgc agcgtgtcga gcgcatcgtt gatctgatcg agcgggaatt
    16321 tcgccgtcac cagctcggcg accttgatct ttccctgccg cgccaattcg aggacgcgcg
    16381 gatagtccac ggcgcgacag ccaagcgagc cgatgatctc aatctcacga aacatgacct
    16441 ttccggtgtt gagcgtcatc ggcttatccg acgagccgac gacgacgaac cgcccgccgg
    16501 cgtgcaccga gttaaacgct tgctcctgcg tgatagggtt gccgatgcac tcgaacgcga
    16561 tgtccgcgcc gccgcccgtc agtttgcgca attccttgtc cagccgctcg acggccttgg
    16621 gattgaccgt ggcctttgcg cccagcttgc gcgcccactc cagcttgtga tcggcgatgt
    16681 ccaccgcgat cacctgcccg ccgacagccg ccgcaatctg cacgcagttc aagccgatgc
    16741 cgccacatcc gatgacgacg acggcgtcgc cgggttgaac gcgcccgcga ttcacgaccg
    16801 catgaaaggg cgtcgtcgtc gcgtcggcaa tgatcgcacc ctcgacgagc ggaatctccg
    16861 gtggcagcgg gatggcatcc ttggccggcg agatgatgta ctcggcgtaa gcaccgtcca
    16921 cgtaactgcc gaacatcacc atgttttcgc agatattctc gcgaccggtg cggcacatgc
    16981 gacaatggcc gcacccgtac acggtgggca gcagcaagcg atcgccttcc ttccaccccc
    17041 ccctttcccc ccccgtcgct gacggggggg gactaagggg ggggccaact gcggccaccg
    17101 tgccggaagt ctcgtgcccc agcaccagcg gcggtttttt gaacgtcggc acgtcgtggt
    17161 cgatgtagtg aaggtcggtg tggcacacgc cgcatccggc gaccttcacc agaatctcgc
    17221 ccgccttcgg ttcgggcgtc ggaatgtctt cgatggtgag cggctggtgc gctccatgaa
    17281 aaatggctgc tttcatgtta tctcctgaaa tctttcgtag tggcgacttc agtcgctgtc
    17341 ttgcctgacg cctgaagtcg tcactacggg cttgacttct tcgtatcgag cgacggcctc
    17401 gcgcaggggt tcaggcacac gcatcttctt gcgcgtgtcg cgctcgatca tcacgacggt
    17461 gatctccccc tcggccacgc ggcggccacg gctcgcttcg atcacctgaa agtcgaaacg
    17521 catgctcgaa ttgccgatgt gcccgatgcg cgtatggata ttcagcgtct cgtcgaaata
    17581 cgccggcgac aggtagcgcg cccgcgactc accgtacacc atgtcgatgc cttcggcgga
    17641 catccgctcg taggaatagc cgatcgcgcg caggtaatcc gtcacgccga tgtcgaagta
    17701 aaacagatag tggccgaagt tgacgtgacc ctgcaagtcg gtctcgttga accgcacccg
    17761 caccggagtt aagaacttga atggctcgtg catctctttt atcgacgtat gcacacgaaa
    17821 gggcacgaag attgaaaact tggtgtatct tcgtgtgtct tcgtggatca ctccttcaac
    17881 gtctggtctg taatatcgac gccgtaatcg cgcagcgcgc tctcgcgcga caccttgccg
    17941 tctcgcacat cgcacagcag cgcctcgcga tcgcgctcct ttggatcgcc gtatccaccg
    18001 cctcccgctg catccatgat cacgacatcg cccggtcgta ggcgcgtcag gctgtatggg
    18061 ttgccgggct ggccattgac gaggaatcgc gctcttgctc ccggcaggcc gccgaataag
    18121 ccttcgggcg gcaggcggaa gcgcccggat tggatgccga gattcacggg cggttggggc
    18181 gcgtgctcgt cgttcggcac gcagaagacc tcccgacggc cgaggccgcc gcgttgttgg
    18241 cccggcccgc ccgagtccgg caacagctcg cgccgttcca cgatcaaggg cgtgtcgctc
    18301 tcaagaatct ccacgggcgt attggctccg ttcgccggga agatgaaaca accttctcca
    18361 tcgcgaaggc cgccggcccc catgcccccg ccgcgaatca ataccgcgtg aaatggttgc
    18421 ccattgttgc gccacccgta gaagacttgc atcgtcgccg gcgtgccgcc gcttccgact
    18481 atcacgcggt tcggcaacgc ctgcgccagc gcgcggtaaa tgatctcggt catgaagtga
    18541 ccgatctgca tgcgcgccgc gaccgccgct gggaaacggc agttgacgac tgttccttcg
    18601 ggcgctttca aatggatcgg cgcggcgctc ccgtcgttgt tgggaatctc aggatcgaag
    18661 atactcttga tcgccatgtg gacataggcg taggtgaagt tgtagaccac gttcccgccc
    18721 cagtccactt gcggcgagga acctgccaaa tccaccgtga tgtcgcttcc ggcaacctcg
    18781 accgcgcatt ggatgaccag atcggcgcgg ccctccattt gctcgatcac gccttcggcg
    18841 cggtagatgc cgggagcgac tttggcaatc gaggcgcgga tgctttgctc gctgcgagcg
    18901 atgatctcgt cggccaaatc gtccagcccg tccagatggt attccgccag catccgcacc
    18961 acctgctcgg cgcacacatg attggaggca atctgcgatc gaatgtcgcc gatcacctgc
    19021 tccggcgtgc gcacgttcca ccggatcatc tcgagcacag cctcgttcag gacgccgcca
    19081 tcgtagagtt tcaccagcgg gatgaacaac ccttcctcga acacgtcgtg gttgtccgag
    19141 gccacccgcc cgccgatatc ggagtggtgc aggacgcagg ccgtgaacgc ggttagacag
    19201 tcgtgataga aaacggggct gatgacacag acgtcgttca agtgcccggc cagcgcccac
    19261 gggtcgttgg tgatgtacac gtcgcccggg cggaaggact ccacggggag ctgccgcacc
    19321 agtttcttga tgcccagcgc catcgcgccc gactgccctg gcgtggcgaa agtgccttgc
    19381 gccagctcgc ggccctgacg gtcggtgaac atgcaagtgt agtcatgtgc gtcgcggagt
    19441 agactggaaa aagccgtgcg ggcgacagcc gcatcagcct cgtccacaat cgagagcagg
    19501 cgtcgccaca ggatttcgag cgtgacaggg tcgaattttc gagtcacggc atcctcaggc
    19561 ccgaagggtc cggcagctca acccacaaga acccaaactc atccaccgag gccatcccat
    19621 cctcgccgac gaccaccgtc gactcgcgct cctcgacaat ggccgggccg gagaaggtcg
    19681 cgccggggaa caattcgtaa cggtcgtata cggtgaaggg aatgaaatcg cgcgcgaggc
    19741 cggagaacgc cttccgctcg cctttgatcg cgtcttgaat gtggccgcct ctgttgggga
    19801 ggcggggcaa ctccagcaag cgcaccggca ggcaggcgcg cacgcgaaag ttgacgaact
    19861 caaccggcga ctcggggtac gtgcgcccgt acagtcgtgt gtaaacttcg tcgaagcggc
    19921 ggcgcacctc caccgggtcg agcgtcgtga aatcgtgttc ggggatcggc aggttggtct
    19981 cggacccctg gccgatgaag cgggcatcca ccgagcgcga gaagtgtatc gagacctcac
    20041 gggtctcgga gacccgtgag gtctgcaacg ttcgctcacc ttcggattcc atctcgcgga
    20101 atagtctctc gattgccgca aaatccgctg agaacaccgg cgctttgtgg ctgcgtacca
    20161 gatcgaacgc gcgcggcgca gtgaagaagc cgagcgccga ccccacaccg gcattgggcg
    20221 gcacgaccac gcgcggcgcg cccagtttct gcgccagccc gtaggcatga accggtcctg
    20281 ccccgccgaa ggcggccagc gtgacgatct tcgggttgcc gccgcgctcg gcgatatgcg
    20341 tcttggcggc ggcggtcatc gtctcgttga tcaaatcgtg gatgccccac acggcttgaa
    20401 gatacgacac acccaacggc ttcgcgacct tttcctcgat gccgcgccgc gcggcttcaa
    20461 catccaactt catcgcgccg ccgaggaagt agtcggcgtc gaggtagccc aatagcagat
    20521 cggcgtcggt gacggtcggc tcggcgccgc cgcgcccata acagatcggc cccggctcgg
    20581 cgcccgcgct ttccggccct acctgcagcg tgcctcgccg gctcatccgg gcgatgcttc
    20641 cgccgcccgc gccgatctcc atcagatcga ccaccggcac ttgaatggtg aggccgctgc
    20701 ccttcatgaa gcgctgcacg cgcccgacct caaaagtggg cacaaccccg gcgacgccat
    20761 tctgtatcag acaggatttg gcggtggtgc cgcccatgtc gaagcagaac atctcggggc
    20821 ttttgaacaa acggctgaaa tactcgcctg cgatgaccgc cgccgtcggc ccagattcga
    20881 tgatgcgcac ggggaactcg gcggcggttt cggcagaagt gacgccgccg ctggaaagca
    20941 tgatgaacag tcgcccgtga aagcccagcc cggctagccg gtcgtacagt cgccgcaggt
    21001 atttttcggt gagcggcttg acgtaggcgt tggcaacggt agtgctggtg cgctcgtatt
    21061 cccgaatttg cggcaagacc tcgtacgaga tggagatcga ggtcgacggc aactcctcgg
    21121 cgatgatgcg cttaagcgcg atctcgtgcg ttggattctc aaaagagttg agcaggcaga
    21181 cggccacgga ttccacgccc atgcttccca gagaacgcac ccgggcgcgc gcgtcttcgg
    21241 cgtcgagtag ctttagcacg ctcccgtccg cacggacgcg ctcgttcacc tccagccgcc
    21301 accggcgggg caccagcggc tcgggaaatt cagcgaagac atcgtagggg gcataacgga
    21361 tctcgcgccc cagttcgagc acgtcacgaa agccctgcgt cgtgatcagc ccggtgcgcg
    21421 cccctttgcg ctcgataatg gagttgatga ccagcgtcgt gccgtggatg atctcctcca
    21481 aatcattcac atagtctgaa accttgtccg ccagcccgcg aatcccctcc tcgatggcat
    21541 ccgacggatc gcgcggcgtg gtcagacact tgtaagaggt gatctgacct gtggcatcat
    21601 cgagcaagac aaaatcggtg aacgtgccgc cgatgtcaat gccgagtcgc atgccagtct
    21661 ccagtggtca gtcgtccgtg gtcagtcatc agtcgccagc cagcgaccgt catagcacgc
    21721 cacgttcctt cagctcagaa agttgctcgc gaccatagcc gagcacttcg ccgagaatcg
    21781 tctcattgtg ctcgccgagc cgtggaggga acgagagtgt tgcggctgcc ccatcgttca
    21841 cttcggcgac gggagcgaga acaagctcag ttgcggtccg atcgtcgcga acgcgcagga
    21901 gcgaatcgcg cgcgagcggc tccgataaca catcgtttag cgtatttact ctggcgaccg
    21961 ggacgccgat gcgattgagc gccgtgatca gatcgtcaga catcatcgct cgagtgcaat
    22021 cggacatccg ttgattcaac cgctcgacgt cggcgatgcg gcccgcatta cgctggtatc
    22081 ccggtgatga taggccctca aagccaggca gttgtgtgat cgcttcccat tgccgatcgt
    22141 tgccgacggc aatgtagaca tacccatcgc gcgtcgggta gacggaaacg ggggcgaaaa
    22201 actggtgtgt gttgccgcgc cgcgtaacgc gctcgccgag cgccgtgagc atgatcgggc
    22261 cggccatcca cgccagcgcc gagtgataca tcgagatgtc gatgcgagag ccttcgccag
    22321 tcaccgcgcg gcgatacagc gccttcatca gcgcgccgta agcgtgctct gccgcgccca
    22381 gatcgaccat gggcaagccg aacacgagcg gcgggccatc cgggtcgccg gtcagctcca
    22441 tccaacctgc gcgcgcctgc agcaccggat cgtaagcggc ttcgtcgcag ttcgggccga
    22501 agccggtgat gccgagccaa atagtctctg gtcgaatcgt cttgatagtc tcgtagtcga
    22561 caccgagttt ggcataactc gacggtcgat tgtttgtggc gaagatgtcg accggcaact
    22621 tcgcaatgag ttcatgcaga atggcgcggc cctccgggtc ggcgaggttg agcgtaatcg
    22681 cctgcttgcc gcagttgttg gcgaaaaaat agctcgccat gcccggctcg ccaaggacgt
    22741 cccgcccgac atagcgattc ggatcgccgc gccccggcgg ctcgaggcga atcacgcgca
    22801 cgccctcgcg cgccaatcgc tgcgtaagaa acggcagcgt ggttgcctgt tcaagagata
    22861 ggacagtcag atcggaaaaa agtgacatac agatcaatac agatagtgat cagtgaccag
    22921 tcatcagtaa ttactgatta ccgattactg atctggactt tgtccaattt ggccttttcg
    22981 gcccgtttta ggcggtttct gcgacatgtc gccccagata tgggggcaca atgtcgtaat
    23041 gaatgcctat atgtggcgtc aatatgaaaa tggacaa
//
LOCUS       DBNHXB010000333         8491 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_257545, whole genome shotgun sequence.
ACCESSION   DBNHXB010000333 DBNHXB010000000
VERSION     DBNHXB010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8491)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_257545"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="AC162G_13510"
     CDS             complement(<1..554)
                     /locus_tag="AC162G_13510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR5076870.1"
                     /translation="MNIIVPIKQVPNLTDELEINSDGNDLDHDALQYVLNEFDEHAIE
                     EAVLAKEATGGAVTVIGVDTTGELDGALHTALAKGADKAAKVTGEYGRGVDSHTQARL
                     LADAIKGMSPDLVLTGVQAADDRDGQIGPMIAARLGLPYVGVVTGVEVKEGKAVVHKE
                     YSGGVMAEFEVALPMVVGVQAAHQP"
     gene            complement(647..1930)
                     /locus_tag="AC162G_13515"
     CDS             complement(647..1930)
                     /locus_tag="AC162G_13515"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR5076871.1"
                     /translation="MAVGLEKVEPDKIVEEKHLVVEGMDISGHWNRMFEQRVIWDYDV
                     EQLEKVASLPGGESLMWCYGCAKCTAVCPVDIVGDYSPRKIHRKTQMGIDLFTSPDLW
                     LCTTCMNCLRVCPKEVNMIQIMPAVREQVVLDGSVPPELQKAFEDTAKHGNPLGQPQR
                     KRADWVKKAGVPIPIMKDLNRPVDILWYVGSYPSYHPRGMDAARAAARIFHALGIDFG
                     ILGVEEKDDGDSQRLAGEKGLFEMLAEHNIATFSKYEFERMVVTGPHEFNAFKNEYPK
                     YGGDFKILHYTQFLVEHLDRLKPMLKRPINLKVTFHDPCYLGRHNGEYDAPRQLLRAI
                     PGLELVEMGRCRENGYCCGGGGGGMWLDSFSKDHTKMRLSERRVREAVEYGADVLAVC
                     CPFEVSRFEDAAKSTGHDKLLVRDILELLDESMKE"
     gene            complement(2003..2800)
                     /locus_tag="AC162G_13520"
     CDS             complement(2003..2800)
                     /locus_tag="AC162G_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076872.1"
                     /translation="MTGQVVDTETAQEFMAQTLEKASRAELGDIALELEHKSARFRAL
                     LAPEALERLDRQHLRGLLRSVFATRRKADAIVEVVGVDGLREAARQLLYGPNSVQERY
                     QSFVDALAGFWGDVRLARKPLPGTEEADLPENVLCDLAAELLHFTEPDTRWLWTRWMW
                     DPRAGTGALPLVTLDEYDLHGRDAGETYMKVGVAVAFVRATGEAAGFARFGMSNSPFG
                     IDVFLSCVYAVYMYTTLRLRMTQEFNRIVPQLPELSRRLLGVWRIEI"
     gene            complement(2854..3060)
                     /locus_tag="AC162G_13525"
                     /pseudo
     CDS             complement(2854..3060)
                     /locus_tag="AC162G_13525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
     gene            complement(3316..4383)
                     /locus_tag="AC162G_13530"
     CDS             complement(3316..4383)
                     /locus_tag="AC162G_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5076873.1"
                     /translation="MASDTILIIGGGPAGLEAARLVGDLGCKAIVVEKRDHLGGTPIA
                     ENYAALTHGFRDAEEAVDELIRSVTHNPNTDVRLGSEVVAAEGSAGSFNVTIAKTGNG
                     ASQVVEAGAVIIATGFQHFDPGRETQMYGYYEYDDVITLADAEAMLKHHRFVKPSNGQ
                     PPERVCFIQCVGSRDRQIGNEYCSKVCCGISSKQAIEVRQQVPNAKVFIFYIDMRMYG
                     YWENEIYWPAQEKYHVQYVKGIITEIIRKGDRLLVRGEDTTMGRPMEVPMDVVVLAVG
                     MEPSRGTKDVARVFGLKQNKYKFVDVPHESLDPTATSAPGIFVAGAAAGPKDLDDTMA
                     MAGAAAAKAVALVRTMTRVTA"
     gene            complement(4513..5718)
                     /locus_tag="AC162G_13535"
     CDS             complement(4513..5718)
                     /locus_tag="AC162G_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR5076874.1"
                     /translation="MSNTSGFIPYDAVNAKSSRKFEREAEYAPEDFHEKVFELEAEGE
                     WIVQRVPEPYVEVMTKYGRKKKIPLELTWHHKSCGQCGHIPGYSTSIFWLNRKLNFKY
                     IDPTDQTSCTAWNYYASATSNAPAQAGVAMRNFAAAYETGYFPIIHCGTSYGHYKEVR
                     EELMHHVETRHQTRDVMRKLGKPMVMPEEIVHYSEWVHAVRDRIAAEQKRDFSKLAVT
                     VHPACHYYKLVQEDAIYDPDIYGGQRTATVSSLIAALGAQVRDYSTWYDCCGFGFRHI
                     LVQRDFTRSFATRRKIQVMKEEADPDVVITHDTGCVTTLDKSQFVGQAHGLNVGVPVM
                     SDSQFAALAMGAHPYRVCQLHWHSTEYRPLLEKMDIDHEKAWAEFQDDLKDLKSGKKE
                     YLTWEDIDA"
     gene            complement(5807..6496)
                     /locus_tag="AC162G_13540"
     CDS             complement(5807..6496)
                     /locus_tag="AC162G_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5076875.1"
                     /translation="MGLFADNYPDVPYETKLRLWEEVKADARFPSYLYGCYECGICVA
                     ACPSARFYDFSPRKIAQAAAREDIDLIYEQMNDDVWNCSQCFSCNRCPRQNSPGGLIT
                     ILREVAVKNGLKSAKQALEGYGRVIYKIMGTGTQVSPDMLQPDAFPDWGPQVRDVSAE
                     LDVWRRALPPETLHTTSTGWQVDDKTIIELYLIWHNTGVMDMIAEVDEGLHMILNDVM
                     EEKLEEAGYEV"
     gene            complement(6580..6963)
                     /locus_tag="AC162G_13545"
     CDS             complement(6580..6963)
                     /locus_tag="AC162G_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR5076876.1"
                     /translation="MDDETKKILYVQTHGVDMPERSATPFYLAAAGAAMDAEVGIYFT
                     MNGPTLLQKGVPETLIVPKKGGGGKELMYFIRQALDCGVRLYVCQPSLDLHALKLEDL
                     IDGVEMIGGAAFNDMALSADAVIAF"
     gene            complement(7031..7552)
                     /locus_tag="AC162G_13550"
     CDS             complement(7031..7552)
                     /locus_tag="AC162G_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HIR5076877.1"
                     /translation="MSKSDPTAPNRLALIASKGTLDWAYPPFILASAAVSMGWDVGVF
                     FTFYGLTLLLKDLGPAVSPLGNPAMPMKMPFGPQGFQKIDWPIPVLMQGLPGFSALST
                     GLMKQTFKNKGVASIPELRAVCAEAGVNMIACTLTLDVFGFKREDFIPEATFGGAATF
                     LEYAKDADVQLFI"
     gene            complement(7651..7890)
                     /locus_tag="AC162G_13555"
     CDS             complement(7651..7890)
                     /locus_tag="AC162G_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR5076878.1"
                     /translation="MTTLQADKVLDCTGTLCPLPVIKTSKAIKEIEIGQVLKMIATDP
                     GAPPDMDAWCRQTGNEMLDSHQEDGKFVFYFRRVK"
     gene            complement(7919..8254)
                     /locus_tag="AC162G_13560"
     CDS             complement(7919..8254)
                     /locus_tag="AC162G_13560"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5076879.1"
                     /translation="MQTSLRREINQLHAEICQALSDPTRILILYELRDGPRNVSELAE
                     SIDAPQPTISRHLKILRDRRVVLAERNGTSIYYSLADSRVVKALDLLREVLAGSIEQR
                     QALIEEIAT"
BASE COUNT         1536 a         2641 c         2497 g         1817 t
ORIGIN      
        1 ggctggtgcg cggcctgcac tccgacgacc atcggcaagg ctacttcgaa ctcggccatc
       61 acgccgcccg agtactcctt gtggacgacg gccttgcctt ctttgacctc gacgccggtc
      121 acgacgccga cataaggcag tcccaggcgc gcggcgatca tcggcccgat ctgaccgtcg
      181 cggtcgtcgg ccgcctgcac gccggtgagc accaggtcgg gcgacatgcc cttgatggcg
      241 tcggcaagca gtctcgcctg cgtgtgcgag tcgacgccgc gtccatattc gccagtgacc
      301 ttcgccgcct tgtctgcgcc tttggcgagc gccgtgtgca gcgcgccgtc aagttcaccc
      361 gtcgtatcca cgccgatcac ggtcaccgcg ccgccggtcg cttccttggc cagcaccgcc
      421 tcttctatcg cgtgctcgtc aaactcgttg agcacgtatt gcagtgcatc gtgatccagg
      481 tcgttgccgt cactattgat ttcgagttcg tcggtgagat tggggacttg tttgatggga
      541 acgatgatgt tcatggttat cagtggtcag tgatcagtga tcagtaatca gtcgccggta
      601 caccctgact gctcactgat aactgataac gatgactgat cactgactac tccttcatgg
      661 attcatccag cagctcgaga atgtctcgaa cgagcagctt gtcatgaccc gtgctcttcg
      721 ccgcatcttc gaagcgcgag acttcgaacg ggcagcacac agccagcacg tccgcgccgt
      781 actccacggc ctcgcgcacg cgccgctcgg acaggcgcat cttggtgtgg tccttgctga
      841 acgagtcgag ccacatgccg ccgcctccgc cgccgcagca gtagccattt tcgcggcagc
      901 ggcccatctc gacaagctcg aggccgggga tcgcacgcag gagctggcgc ggcgcgtcgt
      961 actcgccgtt gtggcgaccc aggtagcagg ggtcgtggaa tgtcaccttc aggttgatcg
     1021 gtctcttcag catcggcttg agccggtcga ggtgctcgac caggaactgc gtgtaatgca
     1081 ggattttgaa gtcgccgccg tatttcgggt actcattctt gaacgcgtta aactcgtgcg
     1141 ggccggtcac gaccatgcgc tcgaactcat acttgctgaa tgtggcgata ttgtgctccg
     1201 ccagcatttc gaacaagccc ttctcgcccg ccagccgctg cgagtcgccg tcgtccttct
     1261 cttcgacgcc caggatgccg aagtcgatgc ccagcgcgtg gaagatgcgc gcggcggcgc
     1321 gcgccgcgtc catgccgcgc ggatggtacg agggatagga gccgacatac cataggatgt
     1381 ccaccgggcg gttgaggtcc ttcatgatcg ggatgggcac gcctgccttc ttcacccagt
     1441 cggcccgctt gcgctgcggc tggccgagcg gattgccgtg cttggcggtg tcctcgaatg
     1501 ccttctgcag ctcgggcggc acgctgccgt cgaggacgac ctgctcgcgc acggcgggca
     1561 tgatctggat catgttcact tctttggggc acacccgcag gcagttcatg cacgtcgtgc
     1621 acagccacaa atcgggcgag gtgaacaggt cgatgcccat ctgcgtcttg cggtgaatct
     1681 tgcgcgggct gtagtcgccc acgatgtcga ccgggcacac ggcagtgcac ttcgcgcagc
     1741 cgtagcacca catcaggctc tcgccgcccg gcagcgacgc gaccttttcg agctgctcga
     1801 cgtcgtaatc ccagatcacg cgctgctcga acatccggtt ccagtgaccg gagatgtcca
     1861 tgccctcgac gacgagatgc ttttcttcaa cgatcttgtc gggttcaacc ttttcgagac
     1921 cgacggccat cttccctccc gatcaccaat taccaataac caatgacttg tgaggtgatt
     1981 agttattggt aattggcaat tctcatatct ctattctcca tacacccaac aacctgcgcg
     2041 agagttcggg cagttgcggg acaattcgat tgaactcttg cgtcatgcgc aaccggagtg
     2101 tggtgtacat gtacacggcg tacacgcatg agagaaagac gtcgatgccg aatgggctat
     2161 tgctcatacc gaacctggcg aatccggccg cctcgccggt cgcgcgcacg aaagcgaccg
     2221 ccacgcccac cttcatgtac gtctcgcccg catctcgccc gtgcaggtca tactcatcca
     2281 gcgtcacgag cggcagcgcg cccgtgccgg cgcgcggatc ccacatccag cgcgtccaca
     2341 gccagcgtgt atccggttcg gtgaaatgca gcagctctgc ggccaggtcg cacagcacgt
     2401 tttccggcag gtcagcttct tctgtgccgg gtaatggctt gcgcgccagt cgcacatcgc
     2461 cccaaaagcc ggcgagagca tctacaaacg attgataccg ttcctgcaca gagttcgggc
     2521 cgtacagcaa ctgccgcgcc gcctcgcgca gcccatccac gccgactact tcgacgattg
     2581 catcggcctt gcggcgagtg gcgaagaccg agcgcagcaa gccgcgcaga tgctgccgat
     2641 cgaggcgctc gagcgcctcc ggcgccagga gcgcccgaaa gcgcgcgctc ttgtgctcga
     2701 gttctaaggc gatgtccccc agctccgccc ggctcgcctt ctccagcgtc tgggccatga
     2761 attcctgggc ggtttcggta tcgacaacct gtcctgtcaa acttcaaact ccaaacatca
     2821 aatctcaaaa tcgggctggc gccttatcca acgtcagtcg tcggacttgc tgaggttgtt
     2881 gatcacactg ctcagtatct tgacgatctc aaggctttcg ccgatcaggt agtcgagttc
     2941 atcagagacc acgacttcgg cttccttgat tgtctgtaac cagtggcggg tttctttcgc
     3001 ttccttgcgt gcaatactga gcttgtggat cttgtccttc ttggattcgg ctgcgtctgc
     3061 ttcctcgacg ttgacccaac ggacgtaccc gctctcagta actgccttgc caactcaacg
     3121 ctggcgacgt ccctcggcat cgtgcgaacg aacttgacaa tccggacccc gaacaggaat
     3181 gttctttcgc ggatatcgaa tggcacgtct ctgccctgag ccaatgtcac ccctccagag
     3241 ggaacttcaa actccaaatc tcaaacttca aattggaggt ttgcactttg aagtttgtag
     3301 tttgaagttt gtagcttatg ccgtcacccg cgtcatggtc ctcaccagcg cgaccgcctt
     3361 ggccgcggcc gcgcctgcca tcgccatcgt gtcgtccagg tctttcggtc cggcagccgc
     3421 gcccgccacg aagatccctg gcgccgaagt cgccgtcggg tcgaggctct cgtgcggcac
     3481 gtcgacgaac ttgtacttgt tttgcttaag cccgaagacc ctggcgacat ccttcgtgcc
     3541 gcggctcggc tccatgccca ccgccagcac gaccacgtcc attggcactt ccatcgggcg
     3601 gcccatcgtc gtgtcctcgc cgcgcaccag caggcgatcg cccttgcgga tgatctcggt
     3661 gatgatgccc ttgacgtact gaacgtgata cttttcctgc gccggccagt agatttcgtt
     3721 ctcccagtag ccatacatgc gcatgtcgat gtaaaagatg aagaccttgg cgttgggcac
     3781 ctgctgccgc acctcgatag cctgcttgga cgaaatgccg cagcacacct tcgagcagta
     3841 ttcgttgccg atctgtcggt cgcgcgagcc gacgcactgg atgaagcaca cgcgctcggg
     3901 tggctggccg ttggagggct tgacaaaccg gtgatgcttg agcatggcct cggcgtcggc
     3961 cagcgtgatc acgtcgtcgt actcgtagta gccgtacatc tgcgtctcgc ggccggggtc
     4021 aaagtgctga aagccggtgg cgatgatcac ggcgccggct tcgacgacct ggctcgcgcc
     4081 attacccgtc ttggcgatgg tgacattgaa gctgcccgca cttccttctg cggcgacgac
     4141 ctccgagccg agacgcacgt ccgtattggg attgtgcgtg acgctgcgga tcaattcgtc
     4201 cacggcctcc tccgcatcgc ggaagccgtg cgtgagcgcg gcatagttct cggcaatcgg
     4261 tgtgccgcca aggtgatctc gcttttcgac cacgattgcc ttgcagccga ggtcgccaac
     4321 caggcgcgcc gcctccagac ccgccgggcc gccgccgatg atgaggatgg tgtctgatgc
     4381 catgtgccct ccagtggtca gtaatcagtg accagtgacc agtgatcagt gatcggttgt
     4441 gaggttcctg actgataact gctcactgat aactggtgac tgataactgg tgactgatga
     4501 ctgatgactg aactatgcgt cgatgtcttc ccaggtgaga tactctttct tcccgctctt
     4561 gaggtccttc aggtcatcct gaaactccgc ccacgctttc tcgtggtcga tatccatctt
     4621 ctccagaagc gggcggtatt cggtcgagtg ccagtgcaac tggcaaacgc ggtagggatg
     4681 tgcgcccatc gccagcgcgg cgaactgcga gtcggacatg accggcacgc cgacgttcag
     4741 accgtgcgcc tgcccgacga actggctctt gtcgagcgtc gtcacgcagc ccgtgtcgtg
     4801 cgtgatgacc acgtcgggat cggcctcttc cttcatgacc tggatcttgc gccgcgtggc
     4861 gaacgatcgg gtgaagtcgc gctggacgag gatatgccgg aagccgaagc cgcagcagtc
     4921 gtaccacgtc gagtaatcgc gcacctgcgc gccgagcgcc gcgatcagcg acgacacggt
     4981 cgccgtgcgc tggccgccgt agatgtccgg atcgtagatc gcgtcctcct gcaccagctt
     5041 gtagtagtga caggccggat gcacggtgac ggcgagcttg gagaagtcgc gcttttgctc
     5101 ggcggcgatg cgatcgcgca cggcgtgcac ccattcggaa tagtggacga tctcctcggg
     5161 catgaccatc ggcttgccga gcttccgcat cacgtcgcgc gtctgatgcc gagtctcgac
     5221 gtgatgcatc agctcctcgc gcacttcttt gtaatggccg taggaagtgc cacagtggat
     5281 gatggggaag tagcccgtct cgtaagccgc cgcgaaattg cgcatcgcga cgccggcctg
     5341 cgccggagcg ttcgaggtgg ccgaggcgta gtagttccac gccgtgcacg aggtttgatc
     5401 ggtcgggtcg atgtatttga aattcagctt ccggttgagc cagaagatcg acgtcgagta
     5461 gccggggatg tggccgcact gcccgcacga tttgtggtgc caggtcaatt cgagcgggat
     5521 tttcttcttg cggccgtact tcgtcatcac ttcgacgtaa ggctccggca cgcgctggac
     5581 gatccactcg ccctccgctt ccagctcgaa gactttctcg tggaaatcct caggcgcgta
     5641 ttctgcctca cgctcgaact tgcgcgacga tttggcattc acggcgtcgt aggggataaa
     5701 accggatgtg ttgctcacag ttgttcctcc acttggcttg tcgcatatcg cacatcgcgt
     5761 atcgtttatc gcccagtgat cacgcatcac gcatcgcgtt tcgcgctcac acctcgtacc
     5821 ccgcttcttc cagcttctct tccatcacgt cgttcaagat catgtgcagg ccctcatcga
     5881 cctcggcgat catgtccatc acgcccgtgt tgtgccagat gagatatagc tcgatgatgg
     5941 tcttgtcgtc cacctgccag ccggtcgagg tggtatgcag cgtctcaggc ggcagcgcgc
     6001 ggcgccacac gtccagctcg gcggacacgt cgcgcacctg cgggccccaa tcggggaagg
     6061 cgtcgggctg gagcatgtcg ggcgacacct gcgtgccggt gcccatgatc ttgtagatga
     6121 cgcgcccgta gccctcgagc gcctgcttcg ccgacttgag gccattcttc acggccactt
     6181 cgcgcaggat ggtgatcaag ccgcccggcg agttctggcg cgggcagcgg ttgcacgaga
     6241 agcactgcga gcagttccac acgtcgtcgt tcatctgctc gtagatcagg tcgatgtctt
     6301 cgcgagcggc cgcctgcgcg atcttgcgcg gcgagaagtc gtagaaccgc gccgaaggac
     6361 aggccgccac gcagatgccg cattcgtagc agccgtacag atacgagggg aagcgcgcgt
     6421 cggccttcac ctcttcccag agacgcagct tggtctcgta aggaacgtcg gggtaattgt
     6481 ctgcgaatag gcccatgttt gtctccagtg atcagtcatc agtgatcagt tatcagtcgg
     6541 atgactgata actgatcact gacaactgat aactgatcac taaaaggcga tgaccgcatc
     6601 cgcgctcagc gccatgtcgt tgaaagctgc cccgccgatc atctccacac cgtcgatcaa
     6661 gtcttcgagc ttgagggcgt gcaggtcgag cgacggctga cacacataca gccgcacgcc
     6721 gcaatcgagc gcctgccgga tgaagtacat aagctccttg ccgccgccgc ctttctttgg
     6781 gacgatgagc gtttcgggca cgcccttttg cagcagcgtc gggccgttca tcgtgaaata
     6841 aatgccgacc tcggcatcca tcgccgcgcc ggcggcggcc agatagaacg gcgtggccga
     6901 tcgttcgggc atatccacgc cgtgtgtttg aacgtataaa atcttcttgg tctcgtcgtc
     6961 cacaggacct cccacagttc cttgagttcc tgacagggga actcaaggaa ctgagcacgg
     7021 cgataggcga tcagatgaag agctgcacgt cggcgtcttt ggcgtattcg aggaaggtgg
     7081 ccgcgccgcc gaacgtcgcc tccgggatga aatcctcgcg cttgaagccg aacacgtcca
     7141 gcgtcagcgt gcaggcgatc atgttcacgc cggcctcggc gcacacggcg cgcagctcgg
     7201 ggatggacgc gacgcctttg ttcttgaaag tctgcttcat caggcccgtg ctgagcgcgc
     7261 tgaagccggg caatccctgc atcagcaccg ggatcggcca gtcgatcttc tggaaaccct
     7321 gcgggccgaa cggcatcttc atcggcatcg ccgggttgcc tagcggcgat acggcgggac
     7381 cgaggtcttt caagagcaga gtcaggccgt agaacgtgaa gaacacgccc acatcccagc
     7441 ccatgctgac ggcggcgctg gccaggataa agggcgggta agcccagtcc agcgtgccct
     7501 tgctggcaat gagcgccagg cggttcggtg cagtcggatc cgacttagac atggttgcct
     7561 cctgttggcg tatcgcatat cgcttatcgc atatcgtgta tcgcgtatgg cctattaccg
     7621 atcgataagc tataagcgat tcgcgattcg ctatttcacg cggcggaaat aaaaaacgaa
     7681 cttgccatct tcctggtgcg aatccagcat ctcgttgccg gtctgccggc accacgcatc
     7741 catgtcgggc ggcgcgccgg ggtcggtggc gatcatcttg agcacctgcc cgatctcgat
     7801 ctctttgatg gctttggagg tcttgatgac cggaagggga cacagcgtgc ccgtgcagtc
     7861 gagaactttg tcggcttgaa gtgtggtcat atctcctctc ctgtatgctt caactgctct
     7921 aggtggcaat ctcctcgatc agcgcctgcc gctgctcgat ggacccggcg agaacctcgc
     7981 gaagcaggtc cagcgctttg acgacgcgcg agtccgccag agagtagtag atactggtcc
     8041 cgtttcgttc ggcgagcacc acgcggcgat cgcgcaggat tttcagatgg cgtgagatag
     8101 tcggctgcgg agcgtcgatc gactcggcca gttcgctcac gttgcgcggc ccgtcgcgca
     8161 gctcgtagag gatcaggatg cgcgtcggat cggagagtgc ctggcagatt tcggcatgca
     8221 gttggttgat ttcgcgccga agcgacgttt gcatggctga gtcctgcgag ggaatatctg
     8281 ctttgccgaa taaacgaatg gatgtgcgca ttatagccgc aatcgcagga ggttttcatc
     8341 ctccccagcg gggataacgt ctcccccgtg cgggggattt tggaaagact tcggaagtct
     8401 tcaaggccct tccccggtcg caagccctgc gcagacttcc gaagtctgga aatgtaggcg
     8461 tcattcgagg gtgatcagtc ccattctcac a
//
LOCUS       DBNHXB010000334         3597 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4619238, whole genome shotgun sequence.
ACCESSION   DBNHXB010000334 DBNHXB010000000
VERSION     DBNHXB010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4619238"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2935)
                     /locus_tag="AC162G_13565"
     CDS             complement(<1..2935)
                     /locus_tag="AC162G_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5076880.1"
                     /translation="MRRKMQRAIHLLTVTAILLSLVGLAPASATRPSANLARLAPAST
                     TEAAPLAARPSSNQPDGPAGVASASEKLIQLKVAPDLRDAVRKADDAVLSVFMTIKAG
                     YDPVPAMVRGVVRPLVVADQQLAFGQIRAIDLVKLLGDENVLAVQKVDFNMTAAPPPR
                     DPELGGHPLPTGELRAHLAALGGNDVWYSAAPQRDVRPADWRDVLTDTHKSKLAWDKG
                     YTGVGVKVATLDDGVDFAHPDLQGTFARIPITTTAWTTATNPYYDAGAGLAWPYVFSP
                     ISSLYYAYDYWFGLSFVEDCAAGTHLVDTSATPLAVSLIPGTKQATFTPNSSCSDTFT
                     HNYIFSDTSLSGVYHFGSHPDHNLIDLYDERPAVLVVDEGRYGHAAGVYDTVYVDLDN
                     DYDFRDEKPMTIASPESYRDLWDIDYVPGPDGFADLSGGLLYWISDGASPVPGTDWMW
                     GVGAFGVDEGPGDLVGLEGPFDSGYSHGTQLTSNIVGQGRINGYHWDFADVGTPAAGV
                     LGHAPGAKSVAITNIYRNFGSSKLDGYLMASFGPDGVSNSGDEVQITNNSYGSSDQDN
                     DNWEYDGRFIANLQRNLAPNTVHMFSTGNGAPGYGTVAPPSPSTGIGVCASTQFGSTG
                     WDNPENASQILANDIVQWSNRGPGARGNNGVDVCANGAWGGGIEALNYFGANGAASWS
                     TWGGTSRSSPVSAGIMALIYQAYRQEFGVWPDYDTARALLKSSSTNVNYNALVQGAGV
                     LNALRGVEVAGGEYGVYVTPDEWNPGDYRGTDYPAFAHLVSAGDTLTRSITVHNTGAT
                     TITVGIRDGEHRRIGSTSFTRVTSNANETAPTFNAPNYLIPITSTVAAYQNIAVPADT
                     KLMVVRENFPYSQFDPDGNYNQNQNWRLLVYNWSDNNSDGNLWTDTNLNSSVNYSTTV
                     WNGTTCVNIDFFPCVTGGELDAGEYVRFGYHRAFANNHQLWVHDPLARMDDGLFIGLQ
                     HSQVSS"
BASE COUNT          679 a         1088 c         1156 g          674 t
ORIGIN      
        1 cgctggatac ctgggagtgc tgcaggccga tgaacaggcc gtcgtccatg cgcgccagcg
       61 gatcgtgcac ccacaactgg tggttgttgg cgaacgcgcg gtgatagcca aagcggacgt
      121 actcgccggc gtccagctcg ccgcccgtga cacaggggaa gaagtcgatg ttgacacagg
      181 tcgtgccatt ccagacggtg gtgctgtagt tcaccgagct gttgaggttg gtgtcggtcc
      241 acaagtttcc atcggagttg ttgtccgacc agttgtagac caacaggcgc cagttctgat
      301 tctggttata gttgccatcc ggatcgaact ggctgtacgg gaagttctcg cgcacaacca
      361 tcagcttggt atccgccggg acggcgatgt tttgataggc ggccaccgtc gaggtgatcg
      421 gaatcaggta gttcggcgcg ttgaaggtgg gggcggtctc gttcgcattc gaggtgacgc
      481 gcgtgaagct ggtgctgccg atccggcgat gctcgccgtc gcgaatgccg acggtaatcg
      541 tcgtggcgcc ggtgttgtgg acggtgatac tccgcgtgag cgtgtcgccg gcgctgacca
      601 ggtgggcaaa ggctggatag tccgtgccac ggtagtcgcc gggattccat tcgtccggcg
      661 tgacgtagac gccgtattcg ccgccggcca cttccacacc gcgcagcgca ttcaggacac
      721 cggcgccctg taccagcgcg ttgtagttca cgttcgtcga actcgacttg agcagcgcgc
      781 gggccgtatc ataatccggc cagacaccaa actcctgccg gtaggcctgg tagatcagcg
      841 ccatgatgcc ggccgagacc ggggacgagc ggctggtgcc gccccacgtg ctccacgacg
      901 ccgcgccgtt ggcgccaaag tagttgagcg cctcgatgcc gccgccccag gcgccgttcg
      961 cgcagacgtc cacgccatta ttgccacgcg cgccggggcc acgattggac cactggacga
     1021 tgtcgttcgc gagaatctgc gaagcgttct cgggattatc ccacccggtc gagccgaatt
     1081 gggtggaggc gcacacgccg atgccggtcg acgggctggg cggcgcaacc gtgccatagc
     1141 ccggcgcgcc gttaccggtc gagaacatgt gtaccgtgtt cggcgccaag ttgcgctgca
     1201 ggttggcgat gaagcggccg tcgtactccc agttgtcgtt atcctggtcg gaggagccat
     1261 agctgttgtt cgtgatctgg acttcgtcgc cgctgttcga aacgccatcc gggccgaacg
     1321 aagccatcag gtagccgtcg agtttcgacg agccgaagtt gcggtagatg ttggtgatcg
     1381 ccacggactt ggcgccggga gcgtgaccaa gcacaccggc cgccggtgtg cccacgtcgg
     1441 cgaagtccca gtgatagccg ttgatccggc cctggccgac gatgttcgag gtcaactgcg
     1501 tgccgtgact gtagccgctg tcgaacgggc cttccaggcc gacgaggtcg ccagggcctt
     1561 catctacgcc gaacgcgccg accccccaca tccagtcggt gccgggcacg gggctggcgc
     1621 catctgagat ccagtacaac aggccgcctg acaggtcggc gaagccgtcc gggccgggga
     1681 catagtcgat gtcccataga tcgcggtagc tctcaggcga agcgatcgtc atcggcttct
     1741 cgtcgcggaa gtcgtagtcg ttgtcgaggt cgacgtagac cgtgtcgtac accccggcgg
     1801 catgaccgta gcggccctcg tcgacgacca gcacggcggg gcgctcgtcg tacagatcga
     1861 tcaggttgtg gtctggatgg ctgccgaagt gatagacacc gctcaggctg gtgtcgctaa
     1921 agatatagtt gtgcgtgaag gtgtcactgc acgaggagtt gggcgtgaag gtagcctgct
     1981 tggtgcctgg aatcaggctg accgccagcg gcgtggcgct ggtgtcgacg agatgtgtgc
     2041 cggcggcaca atcctcgaca aagctgagcc caaaccagta gtcataggcg taatacaacg
     2101 agctgatcgg cgagaacacg tacggccagg ccaggccggc gcccgcgtcg tagtatggat
     2161 tcgtcgccgt cgtccacgcc gtcgtcgtga taggtatccg ggcgaaagtg ccctgcaagt
     2221 ccgggtgggc gaagtcgacg ccgtcgtcga gcgtggcaac cttcacgccc acgccggtgt
     2281 agcccttatc ccaggccagc ttggacttgt gggtgtcggt caggacatcg cgccagtcgg
     2341 caggacgcac gtcacgctga ggcgcagccg agtaccacac gtcattcccg cccagcgcag
     2401 ccaggtgtgc gcgcagctcg cccgtcggca gcgggtggcc accgagctcc gggtcgcgcg
     2461 gcgggggcgc ggccgtcata ttgaagtcga ccttctgcac ggccagcacg ttctcgtcgc
     2521 caagcagctt gaccagatcg atcgccctga tctggccgaa agcgagttgt tggtcggcga
     2581 cgaccagcgg ccgcaccacg ccgcgcacca tcgccggaac cggatcgtag ccggctttga
     2641 tcgtcatgaa tacgctcagg acggcgtcgt cggccttacg cacggcatca cgcaagtccg
     2701 gagccacttt gagctggatg agcttctccg acgcagaggc cacgccggca gggccgtcag
     2761 gttgatttga cgatgggcgg gcagccaatg gcgccgcctc tgtggtcgac gccggagcga
     2821 gccgggccag attggcgctc ggccgcgtcg cactggccgg cgcaaggccc acgagagaca
     2881 gcaggattgc cgttacggtg agcaaatgaa tcgcacgttg catcttgcgg cgcatgattg
     2941 tcctccttga ctcacaatgt aaactcagat tcggctcgtc gcgccgagga cggcgcggcc
     3001 aacgccgccg gggacggcgg cttgagccta aatgtgacat ggtcgaatta caacgtaaac
     3061 tgaaacggat cagccggcca ttcgaatatg cctggttccc gtccggcttc gttgcacctc
     3121 ccttcttgac cgtctacgac gggcaaacaa aaagacatcg tatgggtact ggccccatag
     3181 atgtctcgcc tccgatcagc cgcgcgaatt gcgacaagcc atcctggcaa cagacggcac
     3241 tccggtctaa tgatcgcatg aaccttccac ccgtgcgtgc gtgaccggct taggagagca
     3301 agtcacaagt tttgccgaca atctgccaac aacgtgtgac tacaggccgc aacaacccag
     3361 gagaaccgga ttttaagact tgtgcagatt cttacgacgc gatcgataga gaaacggaac
     3421 ggcgcttaca ttgtgccata gatagccggg catgtcaata tgaacaccac catactatac
     3481 actacgcatg agaatcatcc gcagatgttc gtggattatg tagattctaa ttatcagttc
     3541 ctcagtatca ccggcaggaa gacctgattt ccgccgaccg tgatgaccag cggcccg
//
LOCUS       DBNHXB010000335        11817 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_90361, whole genome shotgun sequence.
ACCESSION   DBNHXB010000335 DBNHXB010000000
VERSION     DBNHXB010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11817)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11817)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11817
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_90361"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="AC162G_13570"
     CDS             <1..818
                     /locus_tag="AC162G_13570"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5076881.1"
                     /translation="FGLPLVSGARYTMPDYVDYAEYYDFDHATTVDVEFYLTYARQCG
                     SPVLELACGTGRLVIPLAEAGFEVYGLDASENMLAHCRRKVEERRLADRVHLTQANMA
                     AFDLPRKDFALAFVAVRSFMHLITQADQLACLERVHRHLRPGGCFVVDVYAPDYEKMA
                     QPPNGPFVLRREFDLPDGHHVIRRDRFVRNDLVRQIQQYEMKFEEYDAAGALVRERTV
                     PMDTRYTFRYELQLLLERAGFDVIDVFRDYDRRPYDGTGEIIAVARRPQPGAE"
     gene            complement(828..2054)
                     /locus_tag="AC162G_13575"
     CDS             complement(828..2054)
                     /locus_tag="AC162G_13575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HIR5076882.1"
                     /translation="MQAEIIATGSELMLGEQVDTNSPYIARRLREIGLPLVYTTHVGD
                     DEPRMAEAIRIALSRSDVVILGGGLGPTVDDVTRSAAARALGRELVYHPELYAQIEAR
                     FKAFGRTPTENNRQQAYIPDGAQPIENPVGTAPGFLIEHEGKLIFSLPGVPRELMHLL
                     DSVVIPHLQAKLDLRDVIVVRTLHTIGEGESRIDSLIADLEQSANPAIGLSAKQGQVD
                     VRLTARAGSRGEAHALLADMESRLRERIGKWVFGADDETLEGIIAALLAHRRLTLATV
                     ELNTGGTISGRLTYNAAAQPLPEAYRGGLVLSDVNALHKTLDVDDDPAGHLAVVTRAA
                     ERVRKIHGTSLGLGVMLRNEAPDGGPGVSMLIALVSDMEAKSIERRYGGHTALAAQWA
                     ASLALGLLWRYLRNDL"
     gene            complement(2315..2815)
                     /locus_tag="AC162G_13580"
     CDS             complement(2315..2815)
                     /locus_tag="AC162G_13580"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR5076883.1"
                     /translation="MNKVEILKRADMFYDLTPAQYEMLAAICDERKARLGEILFEENT
                     PGDEMFVIGRGAVEVLVDPSIVGGPGVKSRTGPVTIATLREGQTFGEVALVDQGLRSA
                     SARCAETETSLLRIPRDKFNALCDSYPELGYKVMRNMAADLAFKIRGSDLAIREQLLW
                     RPRQST"
     gene            3432..4592
                     /locus_tag="AC162G_13585"
     CDS             3432..4592
                     /locus_tag="AC162G_13585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5076884.1"
                     /translation="MAVRAGQFTDSYLPIVNGVSTFIHVFTGALEKLGHEPYVFTFGR
                     ARQAHFAPRVIRSPGLALGRSGYYVAPTYSRRAWSIARSMDVLHAHHPFVAGPLAARL
                     RRQLNKPLVFTNHTRYDYYARVYLPFLPQRPAIGLLGAWMRRFARHCDLIIAVSPAAH
                     MMLKSLGVEAPIEIIYNGIDLKRFARAAPELRSNLGLPPDAFVLMYVGRLGAEKNMDT
                     LLEGYAIARRNAPQAILALVGDGPHTPRLRERVSQLGVADGVRFLDMHPYDRVPSILG
                     AADAFVTASLSEGHPMTIIEALAAGQPVLAFDVAGIRETVIDGENGLLAHVDVQSLAR
                     NMARVATDAGLRARLSAGARRTAARHGIETTAQRIVGHYEYLIRERQKTVAR"
     gene            4606..4977
                     /locus_tag="AC162G_13590"
     CDS             4606..4977
                     /locus_tag="AC162G_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR5076885.1"
                     /translation="MTDRRKALGKLGESLAARELERRGYRIVERNWRCPAGEMDLVAE
                     QRGVLVFVEVRTRRGRERGTPEESLTPRKQAKLIEVAQAYLEGGTADRDWRIDVVAVE
                     LSSRGELLRVDVIENAIEEKG"
     gene            5027..5947
                     /gene="miaA"
                     /locus_tag="AC162G_13595"
     CDS             5027..5947
                     /gene="miaA"
                     /locus_tag="AC162G_13595"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR5076886.1"
                     /translation="MKSLVVIVGPTAVGKTEAAIRLAERIGGEIVSADSRQVYRHMDI
                     GTAKPSAGQRARVPHHLIDVVDPDEALSLAQYQAAAHAAIDDIFRRGRQPLLVGGTGL
                     YVKAVTEGLRIPPVPPDPELRVELEARAAQEGKRALHDELRRVDPAAAARIDPRNVRR
                     TIRALEVYRLSGRRFSEPGSVRPPPYPIVTIGLTMPRPDLYRRIDARVDEMIAAGLIE
                     ETRHLARRYDWSLPAMSGLGYRQIGRFLRGECSREDAIAEIKRNTRGFVRRQYNWFRL
                     TDPRISWLDARFEIVRDLLTIVQGERAAVK"
     gene            5990..6523
                     /locus_tag="AC162G_13600"
     CDS             5990..6523
                     /locus_tag="AC162G_13600"
                     /inference="COORDINATES: protein motif:HMM:NF012708.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR5076887.1"
                     /translation="MFVCPTCGYKNHEGALFCDACGAYFHTGGPLATNAIGADKASRP
                     SPSGFPQPNPPAPSADTVLVLTSDDGQLRFVVDARDGTVLLGRSDRKSRLVVDVDLTG
                     QDGQAYGISRRHARVHFSNGNYLLEDLESLNGTTINGRKLRPYLPEVIHDGDEIRLGS
                     LVLRAAIESPGGPPPGR"
     gene            6627..7697
                     /gene="prfA"
                     /locus_tag="AC162G_13605"
     CDS             6627..7697
                     /gene="prfA"
                     /locus_tag="AC162G_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR5076888.1"
                     /translation="MLEKLAGIEARYDELSHLLADPVVTSDTNRLRTLGQERADLEPI
                     VETFREYKSGRKELDDARALAADTNDDELLSMAQEEVERLETRVAQLESVLRSMLLPR
                     DPRDDKNVIVEIRAGTGGDEAGLFAADLYRMYTRYAENRGWKTALLSANETGVGGFKE
                     VIFEVDGKGAYSRLKYESGVHRVQRVPTTEAQGRIHTSTATVAVLPEAEEVDIQIPEK
                     DIEIEVYKSAGAGGQNVQKNATAVRIRHLPTGIVVQCQDERSQLQNKMRAMSILRARL
                     YEMEERKRLSELTDARRSQVGTGERSEKIRTYNFPQNRVTDHRINLTSYRLETVLDGE
                     LDEFIEELATRDQAEKLQAAQV"
     gene            7699..8586
                     /gene="prmC"
                     /locus_tag="AC162G_13610"
     CDS             7699..8586
                     /gene="prmC"
                     /locus_tag="AC162G_13610"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR5076889.1"
                     /translation="MTAGGAATTVSAALQDAARRLDTARIDAPRIVAEALLAHVLSLV
                     RAQLLARPETPLSLDQLSRYQVLVERCTSGEPLAYVIGHREFYALDFVVDPRVLIPRP
                     ETELLVEMAIRIAGARPAAGGGEYIIADVGTGSGAIAVTLAVHLPFVRVVATDISPDA
                     IAVASQNARRHGVAERITFKVGNLLESIEPPVDLLAANLPYVRTEEWKHLARPIRDHE
                     PAIALDGGPDGMLVVSRLLHDAPRVMRSGGSILVEIGASQGIAAAELARDSFPDADIS
                     VATDHAGLDRLLVVRTPWS"
     gene            8673..9293
                     /locus_tag="AC162G_13615"
     CDS             8673..9293
                     /locus_tag="AC162G_13615"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR5076890.1"
                     /translation="MRTTILKVDEPDAIERAAQMLREHKLVAFPTDTVYGLGALAFSA
                     DDVAALYRAKGRPPERAIAVLIAEPSMVDLVADHIPRVARRLMDRFWPGALTLIVPKR
                     LDVPDVVSGGPTVGVRVPALETTRRLLAVTGPLAVTSANRSGQPSPRSAEEVMLQLEG
                     RIHAVLDGGVTDGVPSTVVDCSRTPPAIVRPGPITEEQLRVVTRLA"
     gene            9459..10007
                     /locus_tag="AC162G_13620"
     CDS             9459..10007
                     /locus_tag="AC162G_13620"
                     /inference="COORDINATES: protein motif:HMM:NF039187.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaI-LiaF-like domain-containing protein"
                     /protein_id="HIR5076891.1"
                     /translation="MESRRNGGTLVVAILLIGIGLFFLLVNTNVVRVSIGQLWPAIPA
                     LIGAALLVQFFTGGMRDPGLVTGGTIFLLTGLFFFLFTLGVTIEPFGQINWGDMAVLW
                     PAIPTIIGVALLMQWLFGGLRDHGLLIPVTLFLIVGLGGFAFTLRGIPAFSILIDYWP
                     ALLIVIGLIVLLRSLRRPQSSQ"
     gene            10105..11205
                     /locus_tag="AC162G_13625"
     CDS             10105..11205
                     /locus_tag="AC162G_13625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5076892.1"
                     /translation="MLIGIDASRAVASQATGTEYYSQHLIRALIELAPERRFRLYFNR
                     PPVGDAYRASHVEHRIMSFPRLWTHVRLAIDVTLHPPDVLFVPAHVLPVVHPRPSVVT
                     IHDLGYLYFPETHPQAERLYLNLSTRWNAQTSARVIADSQATKDDLMRHYGTPPEKIV
                     VAHPGVEPAVRRIEDVQRIQAVKSHYGVEGEYLLYLGTLQPRKNLARLIEAFARSSAT
                     QSRLVIAGKKGWLYDDLFRQVERFGLGHRVIFTGYVPDEDKSALLSGATALVFPSLYE
                     GFGFPVPEAMACGTPVICSSTSSLPETAGDAALLVDPIDVDAIASAIDRMTSDSALRW
                     KLIARGYLQVGRFTWQACAKKILETLDEVARL"
     gene            11207..>11817
                     /locus_tag="AC162G_13630"
     CDS             11207..>11817
                     /locus_tag="AC162G_13630"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008194 - UDP-glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HIR5076893.1"
                     /translation="MRSIDVLGIRVDDVTVEEALDFVASCIAGRAPRQIATVNVEFIM
                     TAQADAEFRDILNRSALNIPDSAGVLWAARRFGPPLRERVAGIDMVEHIARRASQAGW
                     KLFLLGAAEGVAQKATEVLQSRYAGLTVVGAYAGSPRHDEEDEIAERIRSASPDVLFV
                     AYGAPAQDKWIARNLKRLNVPVAMGVGGAFDFIAGITRRAPHW"
BASE COUNT         2045 a         3890 c         3785 g         2097 t
ORIGIN      
        1 cctttgggct gcctcttgtc agcggtgcac gctacactat gcccgactat gtcgactacg
       61 cggaatacta cgatttcgac cacgccacga cggtcgacgt cgagttctac ctgacttacg
      121 cccggcagtg cggctcgccc gtcctcgagc tggcttgcgg cacgggccgg ctcgtcatcc
      181 cgctggccga agccgggttc gaggtttacg ggctggacgc gtccgagaat atgctggcgc
      241 actgccgccg caaggtcgaa gagcggcggc tggcggatcg agtccacctg acgcaggcga
      301 acatggccgc cttcgacctg ccgcgcaagg acttcgcgct ggcgttcgtc gccgtccgct
      361 cgtttatgca tctaatcaca caggccgatc agctcgcctg cctggaacgg gttcatcgcc
      421 acctgagacc tggcggctgc ttcgtcgtcg acgtctacgc ccctgactac gagaagatgg
      481 cccagccgcc gaacgggcca ttcgttttgc ggcgagaatt cgacctgccg gacggccatc
      541 acgtgatccg gagagatcgc ttcgtcagga acgacctcgt ccgccagatt cagcagtacg
      601 agatgaagtt cgaggaatac gacgcggcgg gcgctttggt gcgagagcgg actgtcccga
      661 tggatacgcg ctacaccttt cgctacgagc tgcaattgct gctggaacgt gccggattcg
      721 acgtaatcga cgtctttcgg gattatgatc ggcggcccta cgacgggaca ggggagatca
      781 tcgcggtggc gcgccgtcca cagcctggcg ctgaatgacg ccagcggtta taggtcattc
      841 ctcaagtatc tccacaacag cccgagcgcc agcgacgccg cccactgcgc cgcgagcgcg
      901 gtatgcccgc cgtagcggcg ctcgattgac ttcgcttcca tatccgacac gagcgcgatg
      961 agcatcgaca cgcccggccc gccgtcaggg gcctcattgc gcagcatcac ccccagcccg
     1021 agcgacgtgc cgtgaatctt gcgcacgcgc tcggcggcgc gcgtcacgac ggcgagatgc
     1081 ccggcaggat cgtcgtccac gtcgagcgtc ttgtgtaatg cgttgacatc cgacagcacc
     1141 agcccgcccc ggtatgcctc gggcagcggc tgcgccgccg cgttgtaggt caggcggccg
     1201 ctgatcgtgc cgccggtgtt cagctcgacc gtcgccagcg tcaggcggcg gtgcgcgagc
     1261 agcgcggcga tgatcccttc cagcgtctcg tcgtctgcgc cgaagaccca cttcccgatc
     1321 cgctcgcgca ggcgcgactc catgtctgcc agcagcgcgt gcgcctcgcc gcggctgccc
     1381 gcccgcgccg tcagccgaac gtcgacctgt ccctgcttgg ccgacaggcc gatcgccggg
     1441 ttggccgact gctccaggtc tgcgatcagc gagtcgatcc ggctctcgcc ctcgccgatc
     1501 gtgtgcagcg tccgcacgac gatcacgtcc cgcaggtcga gtttcgcctg tagatggggg
     1561 atcaccacgc tgtcgagcag gtgcatcagc tcgcgcggca cgcccggcag gctgaagatc
     1621 aacttgccct cgtgctcgat caggaacccc ggcgcggtgc cgacgggatt ctcgatcggc
     1681 tgcgcgccgt cgggaatgta cgcctgctgc cggttgttct cggtcggcgt gcggccgaat
     1741 gccttgaagc gcgcctcgat ctgcgcgtac agttccgggt ggtaaaccag ctcgcggccg
     1801 agcgcgcgcg cggcagccga gcgcgtcacg tcgtcgaccg tcgggccaag cccgccgccg
     1861 aggatgacga cgtccgagcg ggacagcgcg atgcgaatcg cttccgccat gcgcggctcg
     1921 tcgtcgccga cgtgcgtcgt gtagacgagc ggcaggccga tctcgcgcag ccggcgcgcg
     1981 atgtacggtg aattggtatc gacctgctcg cccagcatca gctcgctgcc ggttgcgata
     2041 atctcggctt gcatgcgcct ccctcgccaa cccaagatca actggacttt ggccaagcgc
     2101 cggagtgcag cctcacgtgc ccccgcagcg aagcggagcg gggtcaggct gccatgtctt
     2161 gggccgccgc aggctgaagc ctgcactccg aacgccagat atggctcttg ccaaactcca
     2221 ggatcaagtg aacttgaatc gtctgcccac aaacaatcgg acggacgcca cgggcggctc
     2281 cgtccgatcg aggtcgctga acgaagcgat ggcttcacgt gctttgccgc gggcgccaca
     2341 gcagttgctc gcggatggcc agatcgctgc cgcgaatctt gaaggccagg tcggcggcca
     2401 tgttgcgcat gaccttgtag cccagctccg gatacgagtc gcacagcgcg ttgaacttat
     2461 cgcgcgggat gcgcaggagc gacgtctccg tctcggcgca gcgcgccgaa gccgagcgca
     2521 aaccctgatc gaccagcgcg acttctccga acgtctgtcc ctcgcgcagc gtcgcgatcg
     2581 tcaccggccc ggtgcgcgac tttacgcccg gtccgccgac gatggatgga tcgacgagca
     2641 cctcgacggc gccgcgcccg atgacgaaca tctcgtcgcc cggcgtgttc tcctcgaaga
     2701 ggatctcgcc caggcgtgcc tttcgctcgt cgcaaatcgc ggccagcatc tcgtactgcg
     2761 ccggcgtcag gtcatagaac atgtcggcgc gcttgaggat ctctactttg ttcatggctg
     2821 tctccccgca tccgctgaac cgagttgcgt gtattctaaa ctgccagggg tcacaaagta
     2881 aaactttcaa atgctcccgc tggactgcca agtccagcgg aatgtggcgg acttggcagt
     2941 ccgccacgag actacacggc gtgacaaaaa tgcgcgttta ccgccgcgcg cagtatagca
     3001 caaaggaaaa ccgcaagcaa cacgggcgac ctggtgattg cacacatttg ctccccgcgc
     3061 cgtcctcggc gcggaggcga ctgccgggga cggcggtctg agcgacttgt gggtaatcac
     3121 caggggcgca cggctttggt gcctatgcgc tatgctcagc ctgcggttaa gcggaggcgc
     3181 ccgggtatac tcagcgcgat gtaactactc aggcatcatc gcgagtcctc catggcaaag
     3241 aacatcatcc ggtctggcac acatgggcgt gccacacgat cagcgccaga cgtgtcatgc
     3301 cgaggaggaa cgacgaggca tctctacccc ggcgcgccgt gttcgccggt cggagtagag
     3361 attcctcact gcgttcggaa tgacacgcca cagcccgcat ttgacgaagc ggcccgcatc
     3421 cgttactatg catggctgtg cgcgccggcc agttcaccga ttcctatctg cctatcgtca
     3481 atggcgtctc gacgttcatt cacgtcttca caggcgcgct ggagaagctc ggccacgagc
     3541 cgtacgtttt tactttcggg cgcgcgcgac aggctcattt cgccccgcgc gtcatccgct
     3601 cacccggcct ggcgctgggc cgctccggct attacgtcgc gccgacgtat tcacgccgcg
     3661 cctggtcgat cgcccgcagc atggacgtgc tgcacgccca ccatcctttc gtcgccggcc
     3721 cgctggccgc gcggctgcgg cgacaactga acaagccgct cgtcttcacc aaccacacgc
     3781 gttacgatta ctacgcgcgc gtctatctgc cttttcttcc gcagcgtccg gcgatcggcc
     3841 tgctcggcgc ctggatgcgc cgcttcgccc ggcactgcga tctgattatc gccgtatcgc
     3901 ccgcggctca catgatgctc aaatcgttgg gcgtcgaagc gccgatcgag atcatttaca
     3961 acggtatcga cctgaagcgc ttcgcccgag ccgcacccga gctgcgatcc aacctgggac
     4021 tgcccccgga tgcgttcgta ctcatgtacg tgggtcgatt gggcgcagag aagaatatgg
     4081 acactcttct cgaaggctac gcgatcgcgc gtcgcaacgc gccgcaggcg attctcgcgc
     4141 tggtcggaga cggccctcac acgccgcgcc tgcgcgagcg cgtgtcgcag ctcggcgtgg
     4201 ccgacggcgt gcgcttcctg gacatgcacc cgtacgaccg ggtgccttcg attctcggcg
     4261 cggccgatgc cttcgtcacg gcctcgctct ccgaagggca cccgatgacg attatcgaag
     4321 ccctggccgc aggccagccc gtgctcgcct tcgatgtggc gggcattcgc gaaacggtga
     4381 tcgacggtga gaacggactg ctcgcacatg tagacgtcca gtcgctggcg cggaatatgg
     4441 cgcgcgtcgc gaccgacgcc gggctgcggg cgcgcttgag cgcgggagcg cgtcgcaccg
     4501 ccgcccggca cggcatcgaa accaccgcgc agcgcatcgt tggacattac gaatatctca
     4561 tccgggagcg ccaaaaaaca gtcgctcgtt aggcacagcc catccatgac cgatcggcga
     4621 aaagcgctgg gcaagttggg agagtcgctc gccgcgcgcg agctggaaag gcgcggctac
     4681 cggatcgtcg aacgcaactg gcgctgccct gcgggcgaga tggacctcgt cgccgagcag
     4741 cgcggcgtgc tcgtgttcgt cgaagtgcgg acgcggcgcg gccgtgagcg cggcacgccg
     4801 gaagaatcgc tcacgccgcg caagcaggca aagctgatcg aagtcgcgca ggcgtacctg
     4861 gaagggggca ccgccgatcg cgactggcgc atcgacgtcg tcgccgtcga gctgtcttca
     4921 cgcggggagc tgctgcgcgt cgatgtgatc gaaaacgcga ttgaggagaa gggataacca
     4981 cgtattgtag aaaactccgt ggtctccgtg tctccgtggt tagtttatga agagtctcgt
     5041 cgtgattgtc ggcccgacgg ccgtgggcaa aacggaagcg gcgatccgcc tcgccgagcg
     5101 catcggtggc gagatcgtgt cggcggattc gcgccaggtg tatcgccaca tggacatcgg
     5161 cacggccaag ccgagcgccg ggcagcgcgc gcgcgtcccg caccacctga tcgacgtcgt
     5221 cgatccggac gaagcgctct cgctggcgca gtaccaggcc gccgcgcacg cggccatcga
     5281 cgacatcttc aggcgtggga gacagccgct gctcgtcggc gggacgggcc tgtacgtcaa
     5341 ggctgtgacg gaggggctgc gcatcccgcc tgttccccct gatccggaac tgcgggttga
     5401 actggaggcg cgcgccgcgc aggaaggcaa acgggcgctt cacgacgagc tgcggcgcgt
     5461 cgacccggct gccgccgcgc gcatcgaccc gcgcaacgtg cgccgcacca tccgcgcgct
     5521 ggaggtgtat cggctcagcg ggcggcgctt cagcgagccg ggaagcgtgc ggccgccgcc
     5581 ctaccccatc gtcaccatcg gtctgaccat gcctcgcccc gacctttacc gccgcatcga
     5641 cgcgcgcgtc gacgagatga tcgcggcggg attgatcgaa gagacgcggc acttggcccg
     5701 gcgctacgac tggtcgctgc cggcgatgtc ggggctggga taccggcaaa tcggaagatt
     5761 ccttcgtggc gagtgcagcc gagaggatgc catagcggag atcaagcgca acacgcgcgg
     5821 cttcgtgcgc aggcagtaca actggtttcg cctgactgac ccgcgcatca gttggctcga
     5881 cgcaaggttt gaaattgtta gggacttgtt gaccatagtg caaggggaac gggctgcggt
     5941 aaaatagggt ttgatgactt ggctgctcgc tgcaacccaa ggtgtggcta tgtttgtgtg
     6001 cccaacctgc ggttacaaga atcacgaggg cgcgctgttc tgcgacgcct gcggcgcgta
     6061 cttccacacg ggtggcccgc tcgcgacgaa cgcgatcggc gccgataagg catcgcgccc
     6121 cagcccgtcc ggatttcctc aaccgaaccc tccggcgccc agcgcagata cggtgctcgt
     6181 cttgacgagc gatgacggcc agctccgttt cgtcgtggat gcccgtgacg ggaccgtgct
     6241 gttaggccgg agcgatcgca agtcgcggct cgtcgtcgac gtcgatctta ctggccaaga
     6301 cggccaggcc tacggaatct cgcgccggca cgcgcgggtg cacttctcga acgggaatta
     6361 cttgttggag gacctggaga gcctcaacgg gacgaccatc aatggccgca agttgagacc
     6421 ttacttgcct gaagtcatcc acgacggcga cgaaattcgg ctcggcagct tggttctgag
     6481 agcggcgatc gaatcgccgg gtggaccccc tcccggccgc taagcccctc tttcacttct
     6541 tgaccatcct tatccccgcg cttataatta ggttgcaaag atcgaggcga tcgcgcaagg
     6601 tcgcctcgaa ccgtgaacga tgatctatgc tagaaaaact tgcgggcatc gaagcccggt
     6661 acgacgaact cagtcatctg ctcgccgatc cggtggtgac gagcgacact aaccggctgc
     6721 gcacgctcgg tcaggagcgc gccgatctgg aaccgatcgt cgagacattt cgggaataca
     6781 agagcggccg gaaggagctt gacgacgctc gcgcgctggc cgcggacacc aacgacgacg
     6841 agctgctgag catggctcag gaggaagtcg agcgcctcga gacgcgcgtc gctcaactgg
     6901 agagcgttct gcgatcgatg ctcctgccga gagacccgcg ggacgacaag aatgtcatcg
     6961 tcgagatccg cgccggcacg ggcggcgacg aagccggcct gttcgcggcc gacctgtatc
     7021 gcatgtacac gcgctacgcg gagaaccgcg gctggaagac cgcgctcctg tcggccaacg
     7081 agaccggcgt gggtgggttc aaagaggtca tcttcgaggt ggacggcaaa ggtgcatact
     7141 ctcgcctgaa gtacgagagc ggcgtgcatc gcgtgcagcg tgtgccgaca acggaagcgc
     7201 aggggcgcat tcacacctcg acggccactg tcgccgtgct gccggaagcc gaagaagtcg
     7261 atatccagat tcccgagaaa gacatcgaga tcgaagtgta taaatccgcc ggcgcgggcg
     7321 gacagaacgt gcagaagaac gcgaccgccg tgcgcatccg ccatttgcct accggcattg
     7381 tggtgcaatg ccaggacgag cgctcgcagc ttcagaacaa gatgcgcgcc atgtccatcc
     7441 tgcgcgcccg gctatacgag atggaagagc ggaaacgcct gagcgagctg accgacgccc
     7501 gccgctctca agtaggcacc ggtgagcgca gcgagaaaat ccgcacttac aactttccgc
     7561 agaaccgcgt cacggaccac cgcatcaacc tcacgtcgta tcggctcgag accgtgcttg
     7621 acggcgagct ggatgagttc atcgaagagc ttgccactcg cgaccaggcc gagaagctcc
     7681 aggctgcgca ggtgtgacgt gacggcaggc ggcgcggcga cgaccgtgag cgcggcgctt
     7741 caggacgctg cccggcgcct ggacaccgcc cggatcgacg cgccgcgcat cgtcgccgaa
     7801 gcgcttctgg cgcatgtgct tagcctcgtc cgggcgcaac tgctggcccg tcctgagacg
     7861 cccctttccc ttgaccagct ctcccgctac caggtgctcg tcgaacgctg cacatcgggc
     7921 gagccgctgg catacgtgat cggtcaccgc gaattctacg cactggattt cgtcgtcgat
     7981 ccgcgcgtat tgatcccgcg ccccgagacc gagctgctcg tcgaaatggc gattcgcatc
     8041 gccggcgcgc ggcccgcagc cggggggggc gagtacatca tcgccgacgt cggcacaggg
     8101 tcgggcgcga tcgccgtcac gctggccgtg catctgccat tcgtgcgcgt cgtcgcgacg
     8161 gacatctcgc cggacgccat cgccgtcgct tcgcaaaacg cccgccggca tggtgtggcg
     8221 gagcgcatca cgttcaaggt cggcaatttg ctggaatcga tcgagccgcc ggtcgatctg
     8281 ttggcggcca atctgcctta cgtgcgcacc gaagaatgga agcacctggc ccggccgatt
     8341 cgcgaccacg agccggctat cgcgctcgac ggcggaccgg atggaatgct cgtcgtgagc
     8401 cgtctgctgc acgacgcgcc ccgcgtgatg cgttctggcg gatcgatcct ggtggagatc
     8461 ggcgcttcgc agggcatcgc cgcagccgag cttgcgcgcg attcgttccc tgacgccgac
     8521 atcagcgtcg cgacggacca tgcaggactc gatcgcctgc tcgtcgtccg cacaccgtgg
     8581 tcgtaggagg catctccaga tgccgaggcg cggacgcagc gtgttgcccg agtgcgtcgc
     8641 aatcccaggc agagccggtt gaccgtcaat ctatgcgaac caccatcctc aaagtcgacg
     8701 agccggacgc gatcgagcgc gccgcgcaga tgctgcggga gcacaagctc gtcgccttcc
     8761 cgaccgacac agtgtacggc ttgggcgcgc tggccttcag cgcagacgac gtggctgcac
     8821 tgtatcgggc caaagggcgg ccaccggagc gcgcgattgc ggtcctgatc gccgagccgt
     8881 cgatggtcga cctcgtggct gatcacattc ctcgcgtcgc ccggcgcctg atggacaggt
     8941 tttggccggg cgctttgacg ctgatcgtgc cgaagcgcct ggatgtgccc gacgtggtga
     9001 gcggcggccc gaccgtcggc gttcgcgtgc cggcgctcga gacaacccgc cgcctgttgg
     9061 ccgtgaccgg accgctggcc gtaacgagcg ccaaccgctc tggccagccc agcccgcgca
     9121 gcgcagaaga agtgatgctg cagttggaag ggcgcataca tgccgtgctc gatggcggcg
     9181 tgaccgacgg cgtgccttcc accgtcgtcg attgctcccg tacgccgcct gccatcgtgc
     9241 gtccaggccc gattacggaa gagcaattgc gagtcgtcac ccggctggcg tgaccctgct
     9301 cggcccaacg accgcccacg aatccgaacc tccgtgccaa agacctggca ggtcttgtcc
     9361 gccgctattg accatgcgcc ggatcgcaaa gacctgccag gcctgctcgg tgaaggcatg
     9421 ttacaatggg gtcatcctgc catccccgga ggtctcacat ggaatcgaga cgcaatggcg
     9481 gcacgctcgt cgtggcgatt cttctgatcg ggatcggttt gttcttcctg ctggtgaaca
     9541 caaacgtcgt ccgcgtcagc atcggccaat tgtggccggc cattccggcg ctgatcggcg
     9601 cggcgctcct cgtgcagttc tttaccggcg gcatgcgcga tccggggctg gtgacgggcg
     9661 gcacgatctt cttgctcacc ggcctattct tcttcctgtt caccctgggc gtgacgatcg
     9721 aacccttcgg acagatcaat tggggcgaca tggccgtgtt gtggccggcc ataccgacga
     9781 tcatcggcgt cgcgcttttg atgcagtggc tgttcggcgg tctgcgcgat cacggcctgc
     9841 tcatcccggt gaccctattc ttgatcgtcg gcctgggcgg cttcgcgttc acgctgcgcg
     9901 ggattccggc cttttcgatc ctgatcgatt actggccggc gctgctgatc gtcatcggcc
     9961 tgatcgtgct gctgcgatcg ctccgccgac cgcagtcatc gcagtgaaga ttcgacgaag
    10021 gacgaacgac cgtaggcgaa gggaggattc cttcgtcatt tgccgttggc cgttcgtcgt
    10081 tgatcgctcg tcactctgtt tgtcatgttg atcggcatcg acgcgagccg cgccgtcgcc
    10141 tcgcaggcga cgggcacgga atactactcg cagcatctga ttcgggcgct gatcgaactg
    10201 gcgcccgagc ggcgtttcag gctctatttc aaccgcccgc ctgttggcga cgcgtatcgc
    10261 gcgtctcacg tcgagcacag aatcatgtcg tttcctcgcc tctggacgca cgtacgtttg
    10321 gcgattgacg taaccctcca cccgccggac gtcttgttcg tccccgcgca cgtcctgccc
    10381 gtcgtgcatc cgcgcccaag cgtcgtcacg atacacgacc tgggctacct ttactttccg
    10441 gagacgcacc ctcaagccga acgcctgtac ctgaacctat cgactcgctg gaacgcgcag
    10501 acgtcggcgc gcgtcatcgc cgattctcag gcgacgaagg atgacctgat gcgccactac
    10561 ggcacgccgc ccgagaaaat tgttgtcgct catccgggcg tcgagccggc ggtgcggcgc
    10621 atcgaagacg tgcagcgtat tcaggctgtg aaatcgcact acggcgtcga gggcgagtac
    10681 ctgctttatc tgggcacgct gcagccgcgc aagaacctgg cacgcctgat cgaagcgttc
    10741 gcccgttcga gtgccacaca aagccggctc gtcattgcgg gaaagaaggg ctggctctac
    10801 gacgacctgt tcaggcaagt tgagcgcttc ggcctgggtc accgcgtgat cttcaccggc
    10861 tacgtgccgg acgaggacaa gtccgctctg ctcagcggcg ccacggcgct cgtcttccct
    10921 tccctctacg agggatttgg gttccccgtg cccgaagcga tggcttgcgg cacgcccgtc
    10981 atttgcagta gcacttcgtc gctgccggaa acggcgggcg acgccgcgct cctcgtcgat
    11041 ccgatcgacg tcgacgccat cgcctctgcc atcgatcgaa tgacatcaga ctccgcactg
    11101 cgttggaaac tgatcgcgcg cggctatctc caagtcggca gattcacgtg gcaggcgtgc
    11161 gcgaagaaaa tcctggagac actggatgag gtcgcccggc tgtgagatga gatcgatcga
    11221 tgtgctcggc atccgcgtag acgacgtgac ggtcgaggag gcgctggact tcgtcgcatc
    11281 ctgtattgcc gggcgcgcgc ctcgccagat cgccaccgtc aacgtcgagt tcatcatgac
    11341 tgcgcaggca gacgctgaat tccgcgacat tctgaaccgg agcgcgctga acatccccga
    11401 cagcgcgggc gtgctatggg cggcgcggcg attcggcccg ccgctgcgcg agcgcgtggc
    11461 gggcatcgac atggtcgagc atatcgcgcg ccgggcgagc caagcgggtt ggaagctgtt
    11521 cctgctgggg gcagccgaag gcgtcgcgca gaaagcgacg gaggtgcttc agtcgcgcta
    11581 cgcgggcttg accgtagtgg gggcctacgc cggatcgccg cgccacgacg aagaagacga
    11641 gatcgccgag cggattcgta gcgcaagtcc cgacgtgtta ttcgtcgcgt acggcgcgcc
    11701 ggcgcaggac aaatggatcg cgcggaacct gaagagacta aacgtgccgg tcgccatggg
    11761 tgtgggcggc gcgttcgatt tcatcgccgg catcacgcgg cgcgcgccgc actggat
//
LOCUS       DBNHXB010000336         2440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4285115, whole genome shotgun sequence.
ACCESSION   DBNHXB010000336 DBNHXB010000000
VERSION     DBNHXB010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4285115"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..635
                     /locus_tag="AC162G_13635"
     CDS             345..635
                     /locus_tag="AC162G_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015228540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HIR5076894.1"
                     /translation="MKYFDWDDAKNAKLRAERGIGFEDIVFHIERGDLLDILEHPNAD
                     RYAGQRIFVVRREEYVYLVPFVEDEHTVFLKTIIPSRKATKQYLGEESDDET"
     gene            625..891
                     /locus_tag="AC162G_13640"
     CDS             625..891
                     /locus_tag="AC162G_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HIR5076895.1"
                     /translation="MKLDADEKELVDSVERGEWKSAKGGKRERARYSRYAKATFRKDR
                     RLNIRLSSKDLEAIQKRALAEGLPYQTLISSLLHKYAAGRLKEI"
     gene            complement(1016..1476)
                     /locus_tag="AC162G_13645"
                     /pseudo
     CDS             complement(1016..1476)
                     /locus_tag="AC162G_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087947.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1482..2435)
                     /locus_tag="AC162G_13650"
     CDS             complement(1482..2435)
                     /locus_tag="AC162G_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HIR5076896.1"
                     /translation="MTHERVRLGISACLLGEPVRFDGGHKRDPFLVESLGQFVDWVPV
                     CPEVESGLDAPRESMRLVQADGRIRLLTNKTAQDHTTRMRGYGRRRVEELADGELCGY
                     VLKKDSPTCGLERVKVYGTAGMPVKSGRGLFADALVTRFPLLPVEEEGRLNDPRLREN
                     FVERIFAYRRLNTLFAARWSIGDVVRFHTTHKLTLMAHRPEAYQRLGRLVASGKGMAR
                     PEFQDCYSTEFMTALGAMATPRRHANVLQHMLGYFKQTLDRESRAELLALIGDYAGER
                     VPLVVPLTLFAHHIRRCGVPYLADQVYLRPHPIELMLRNHV"
BASE COUNT          451 a          761 c          795 g          433 t
ORIGIN      
        1 atccgccgcc cggggagatc caagtggtcg tcggatggcg tgcgctactc gagcagccat
       61 agcgcggccg tcgacgccag cgtcctcgcg cgaggcgttg caccgtatac ttacagtctg
      121 cggtgaggtg gccgagtggc cgaaggcggc ggtttgctta ggtcgccaga catcagagaa
      181 ttggcctgaa agtcacgatt ttaggcccgt ttttcattgg tagcttggtt ggcgttggct
      241 gccgattgat ggtcctcgga ccgagtttgg gcacactggc gggcacagtg gagccgacgc
      301 caatgtgtgc ctatcaagca cacatcgtga taccgtagaa ttcggtgaag tacttcgact
      361 gggacgacgc gaagaacgcg aagctcaggg cggagcgcgg catcgggttc gaggacatcg
      421 tcttccacat cgagcgcggc gacttgctcg acatcctgga acatccgaac gccgaccgtt
      481 acgctggcca gcgcatcttc gtggtccggc gcgaggagta cgtgtacctc gtgccgttcg
      541 tcgaggacga gcatacggtt ttcctgaaga cgatcatccc gagccggaag gcgacgaagc
      601 agtacctggg cgaggagtcc gacgatgaaa cttgatgctg acgagaagga gttggtcgac
      661 tctgtcgagc gcggcgaatg gaagtccgca aagggcggca agcgcgagcg cgctcggtac
      721 tcccgttacg cgaaggccac gttccgcaag gatcggcggc tgaacattcg gctgtcgagc
      781 aaggacctgg aggcgatcca gaagcgcgcg ctcgcggagg ggctgccgta tcagacgctg
      841 atatcgagcc tgctgcataa gtacgcggcg ggccgcctca aagagatctg attagaggta
      901 gccccactgc tgtcaaggat cgcaggtgcc cgacgatcgc acccgtcacg tacacacgca
      961 tgtcacaccc ttcctagatc tttcggctgt cgtacgccca gtgaaggacg gcctgtcact
     1021 gtcgcctccg acgttcgaga tcgcccggct cgcagtgctt tcgaatcgcc gccacgtgcc
     1081 gggagatcgc acgctagcgt cgagactgcc tggcatcgac ggcggtccgg cgacccaagt
     1141 agtagcggca gtacactgga accaccccct tggatcctgc ttgtggatcc atccgctgcg
     1201 gcgccagacg cggagcgact gcgaggcgtt cacgccgaag cagttcaggc gcgcgtcatg
     1261 ccgctcggcg cacagcttgg cgcgcgcgaa ccaactgcgt gggaactcct cgtggcagtc
     1321 cgtcatgtac ttcccgccga agacgccgag ccgcagcatc tctttcggcg tcaggtccgg
     1381 cgtgaactcc ggcgcgaagt tccggccgac tggctcggtg cgccagtagg cgtagccgct
     1441 ttgcatgcga tcgttgacga tgactctctt ccccatcgcc gtcacacgtg gttccgcagc
     1501 atcagctcga tcggatgcgg cctcaggtag acctgatcgg ccaggtacgg caccccgcat
     1561 cggcggatgt gatgagcgaa cagcgtgagc ggtacgacca gcggcacacg ctccccggca
     1621 tagtcgccga tcagggcgag cagctcggcc cggctctcgc ggtcgagcgt ctgcttgaag
     1681 tagccgagca tgtgctgcag cacgttggcg tgccggcgcg gagtcgccat ggcgccaagg
     1741 gccgtcatga attcggtcga atagcaatcc tggaattcgg gtcgcgccat ccccttgccc
     1801 gatgccacga ggcggccgag tcgctggtaa gcctccggcc gatgggccat cagcgtgagc
     1861 ttgtgagtgg tgtggaaccg cacgacgtca ccaatcgacc accgtgcggc aaatagcgtg
     1921 ttgagccgac ggtaggcaaa tatgcgttcg acgaagttct cccgcagacg cggatcgttg
     1981 agccgccctt cttcttcgac gggcagcagg ggaaagcgcg tgaccagggc atcggcaaac
     2041 agcccgcggc ctgacttcac cggcatcccg gcggtgccat agaccttgac gcgctcgagt
     2101 ccgcaggtgg gcgagtcttt cttcaacacg tagccgcaga gctcgccatc ggccagttct
     2161 tcaacgcggc ggcgcccgta gccacgcatg cgcgtggtgt gatcctgagc cgtcttgttg
     2221 gtcagcaggc gaatccggcc atcggcctgc accagccgca tcgattcacg cggggcgtcg
     2281 agtccggatt cgacctccgg gcagacgggc acccagtcga cgaactgccc cagcgattcc
     2341 accaggaacg gatcgcgctt gtggccgcca tcgaagcgca ccggctcgcc gagcaggcag
     2401 gccgaaatgc cgagacgaac gcgttcgtgg gtcatcctcg
//
LOCUS       DBNHXB010000337         6529 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_90527, whole genome shotgun sequence.
ACCESSION   DBNHXB010000337 DBNHXB010000000
VERSION     DBNHXB010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6529)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6529
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_90527"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="AC162G_13655"
     CDS             <1..215
                     /locus_tag="AC162G_13655"
                     /inference="COORDINATES: protein motif:HMM:NF014471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR5076897.1"
                     /translation="SPWRRAIQRAIAPIVVALILSGGVILATPGAPDWRLWAIAAATA
                     IGMLTSKANPLWFLAAGGAIGGFLLA"
     gene            complement(190..486)
                     /locus_tag="AC162G_13660"
     CDS             complement(190..486)
                     /locus_tag="AC162G_13660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013954173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR5076898.1"
                     /translation="MSFVLVVNIRIKPENVDKFMQALTGNAREARKEPGCKQFDVLVD
                     PKDKTKVMLYEVYNDEKAFEAHQQMPHFKKYLAEAVPLLASRERQVFTRVEIPR"
     gene            532..969
                     /locus_tag="AC162G_13665"
     CDS             532..969
                     /locus_tag="AC162G_13665"
                     /inference="COORDINATES: protein motif:HMM:NF019301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR5076899.1"
                     /translation="MQTKLVVPQLEPFYRWAEPLSWALVRVTAGLMLIPHGWPKLMAG
                     IGPVAANALAKRGIQPAEPLAVILIAIETIGGLCIALGLFTRFWAAAATIEMAVIVYH
                     HLPKFGWTERGYEYPLMWGLVMFAIALRGGGPYSLDRKLGREL"
     gene            993..1069
                     /locus_tag="AC162G_13670"
     tRNA            993..1069
                     /locus_tag="AC162G_13670"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1027..1029,aa:Arg,seq:acg)
     gene            1590..2285
                     /locus_tag="AC162G_13675"
     CDS             1590..2285
                     /locus_tag="AC162G_13675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5076900.1"
                     /translation="MPENDPRLALVERFFRGTGDTYDIVVDVATFGIDRLWKRRIVRE
                     IPAGAGRILDLACGTGISTMAIARRFPNSRIVGVELREEYLELAREKVRRRNVGNVEL
                     VLSRAEDYRSSEPFDCISSSYLAKYADLPLLVRNCRGMLKDGGVLLIHDFTLPPSRAL
                     RILWRLYFKALQTIGGALLPAWREIYYGLPRLIEETRWLPDLQHALAENGFADVHTQY
                     LTAYGSAIVSARR"
     gene            complement(2298..3083)
                     /locus_tag="AC162G_13680"
     CDS             complement(2298..3083)
                     /locus_tag="AC162G_13680"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR5076901.1"
                     /translation="MGLTAYRAAAKARIDAELKLRAEQWFATLPPPHADALRTILTEG
                     KRLRGSLTCLVAEALGGTLEQALPAALSVEMVQAASLVHDDFVDGDTVRRGRPAAWTL
                     LSSRRAVLVADVMFATAIQTMAQAGAREGATLSHAIAAMAQGALQECGGNTYAEDLDA
                     YRRIIYLKTGSLFAAAARLGALAVDAQETVLQAATEFGARAGEVYQIADDLVDGAQLG
                     SLRPQTQIASLVEQARRALATFPDNQRTRMLADMPQELLRMAA"
     gene            complement(3074..3784)
                     /locus_tag="AC162G_13685"
     CDS             complement(3074..3784)
                     /locus_tag="AC162G_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076902.1"
                     /translation="MSDRRSEWFVPSFGPERFRVAVGLLFLPYTGMVLSFTVIGSMLA
                     PTVHWDRVAAIVLIYFLGLGIGAHAVDAVGGRGARPWGEMFSRSQLWGLAIVSLVGAY
                     SIALWYMVRDTPLLWLIAIPEGFFLLAYNLEWFGGRFHRDAWFALSWGALPVLAGYVL
                     QTNAISASAVVVAVAMALLSLVEIRASRPYKDLKRAMANGRTAEAAHAGHLEGILKSL
                     SAGVIVLGVGLLAFRLWG"
     gene            4314..5582
                     /locus_tag="AC162G_13690"
     CDS             4314..5582
                     /locus_tag="AC162G_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HIR5076903.1"
                     /translation="MRSIFLVIVISGWCIVANVPAWAADQVLIHVNAAAIAGGDGSAK
                     FPYNNLPEAIAAARAASGAPLIKVEPGDYPLAQPLLIDRSMELRGSTEQVASGDSWPS
                     GEAVAGTQTRVFATDALGFQPLVMVGRSDAVVISGVRISGFIFEQATAVGIGVLLNRV
                     QDFWIAENIFRGPAFIGMQSVASSGRVTANHFSGAQAAGASLTGGYPESPSTVDFVGN
                     RSVRNNQGGLLLDGASINIPELGDKLNATVRDNDLSDNVSNLTAFGLRLFILRRDLGA
                     PGDSQSSSRVNALVQGNRIYGNGVGIVIDAGFPYRRVSGVCDSRVYSGRIDLKLAGNT
                     LTASVTTPALVTFTRPTAALNPSSLPQWQYLHAATFAISDRDGTLAQAWIDHPEFDPF
                     VGGLCPADVTHEPLENVLIYNSAELKGRNF"
     gene            6218..>6529
                     /locus_tag="AC162G_13695"
     CDS             6218..>6529
                     /locus_tag="AC162G_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014330906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HIR5076904.1"
                     /translation="MRSIFRAILIAAWCMVANVPAWAADKVPVAIKGYDPVAYFTVGK
                     PTKGSPDIALEWDEHRYLFSTEENRARFKADPARYAPQFSDYCAMALSKGELVEANPE
                     NW"
BASE COUNT         1175 a         2062 c         2071 g         1221 t
ORIGIN      
        1 actcgccgtg gcgtcgcgcg atccagcgcg cgatcgcccc gatcgtggtc gcgctcatcc
       61 tctccggcgg agtcatcctc gccacacccg gcgcgcccga ctggcggctg tgggcgatcg
      121 ccgcagccac ggcgattgga atgctcacct ccaaagcgaa tccgctctgg ttcctcgccg
      181 ccggcggcgc tatcggggga tttctactcg cgtgaacacc tgacgctcgc gcgaggcgag
      241 gagcggcacc gcttcggcga ggtacttctt gaagtgcggc atctgctgat gcgcctcgaa
      301 cgctttctcg tcgttgtaga cctcgtagag catcaccttc gtcttatcct tcgggtcgac
      361 cagcacatcg aactgcttgc agccaggctc cttgcgcgcc tcgcgcgcgt tccccgtcag
      421 cgcctgcatg aacttgtcga cgttctccgg cttgattcgg atgttgacca ctagaacgaa
      481 actcatatgt gctctccttt cggctaatct actcgaacaa ggggagggaa catgcaaaca
      541 aagctggtgg ttccgcagct ggagccgttc taccgttggg ccgagccgct gtcgtgggcg
      601 ctcgtacgcg tcaccgccgg cctgatgttg atcccgcatg gctggccgaa gcttatggcc
      661 ggcatcgggc cggtcgcggc gaacgctctc gcgaaacgcg gcatccagcc ggccgagccg
      721 ctcgccgtga tcctcatcgc aatcgaaacg atcggcggct tgtgcatcgc tctcggcctt
      781 ttcacccgct tctgggccgc cgcagcgacc atcgagatgg cggtcatcgt ctatcaccat
      841 ctgccgaaat tcggctggac cgagcgcggc tacgaatatc cgctgatgtg gggcctggtg
      901 atgttcgcga tcgcgctacg cggcggcggt ccgtattcgc tggaccgcaa gctcggccgc
      961 gaactctagc cgcacggtat aatccgcgcc tcgcgcccgt agctcagttg gatagagtac
     1021 ctggctacga accagggggt cgtgggttca aatcctgccg ggcgcgccaa attctttggt
     1081 acacatcaag cggttggcgt tgtgcgcgtc agccgctttt tgtttcatcg tcacctctgc
     1141 gggcttttgc aggacccctg gtgtcgcata acgattaagg atatgcgcgg gatgcttggc
     1201 gcctcatcgt ctacggcgtc ctgttgtggc tcgccttcca cgtcttcgtc gtggtgtacg
     1261 aagaacccac gctcgagcgg acgtttggga gagagtacga ggcgttccgc gccgcggtcc
     1321 cgcgctggat accgcggatg accccatggc ggtcggaatg attaacggaa cgtccgcttt
     1381 cggccgatag ccgacggcgc gttcaggctc actccaagct gcgctctata atggctgcgc
     1441 aatccggacg cggctcgggt gaagatcgtc gaggcgacgc gctggaccgc gaagcgctgg
     1501 aggacgcgcc acggcagcga agcgcgtcga cacaggctta aggtttagta ttttcagatc
     1561 gtcggcgtgg tgcgtcagtg gcgtcagcaa tgcccgaaaa tgatcctcgt cttgcgctgg
     1621 tcgagcggtt tttccgcggc acgggcgaca cgtatgacat cgtggtggac gtcgcaacct
     1681 tcgggatcga ccggctttgg aagcggcgca ttgtgcgcga gatcccggcc ggggccggcc
     1741 ggattctcga cctcgcctgc ggcaccggga tctccacgat ggcgattgcg cggcgcttcc
     1801 cgaatagccg catcgtcggc gtggagctgc gcgaggagta ccttgagctc gcgcgcgaaa
     1861 aggtacgccg gcgcaacgtg gggaacgtgg agctcgtgct ctcgcgcgcc gaggattacc
     1921 gctcgagcga gcctttcgat tgcatctcgt cctcgtacct tgccaagtat gcggatctgc
     1981 cgcttctcgt gcgcaactgc cgcggaatgc tgaaggatgg cggagtgctt ctcatacacg
     2041 acttcacgtt gccgccgagc cgcgccctgc gcatcctctg gcgcctttac ttcaaggcgc
     2101 tgcaaacgat cggcggggcg ctgctgcccg cctggcgaga gatctactac ggcctgccgc
     2161 ggctcatcga ggagacacgc tggctgcccg atctgcagca cgcgctcgcc gagaacggct
     2221 tcgccgacgt gcacacgcag tacctcaccg cgtacggctc cgcgatcgtc tcggcgcgcc
     2281 gatgagggcg tagggtctca agcggccatg cggagcagct cttgcggcat gtcagcgagc
     2341 attcgcgtgc gctggttgtc gggaaaagtg gcgagtgcgc gccgtgcctg ctcgacgaga
     2401 gaagcgatct gcgtttgcgg tctcagcgag ccaagctgcg cgccatcgac cagatcgtcg
     2461 gcaatctggt aaacctcgcc ggcgcgcgcg ccgaactcgg tggctgcctg taaaaccgtt
     2521 tcctgcgcat cgaccgccag cgcgcccagc cttgcggcgg cggcgaagag cgatccggtc
     2581 ttgaggtaga tgatgcgacg gtatgcgtcg aggtcctctg cgtaggtgtt accaccacac
     2641 tcctgcaacg cgccttgcgc catggctgct atggcgtgcg agagggtggc gccttcgcga
     2701 gctccggctt gtgccatcgt ctggatggcc gtggcgaaca ttacgtctgc gacaagcacc
     2761 gcgcgccgcg acgagagcag cgtccaggcc gctgggcggc cgcggcgcac ggtgtcgccg
     2821 tcgacgaagt cgtcgtgcac cagcgaagcc gcctggacca tctcgactga aagcgcggcg
     2881 ggtaaggcct gctcgagcgt cccgccaagc gcttcggcta ccaggcaggt gagcgagccg
     2941 cgaaggcgct ttccttcggt gagaatcgtg cgcaacgcgt ccgcgtgcgg gggcggcagt
     3001 gtcgcgaacc attgttctgc ccgcaacttc aattcggcat cgatgcgcgc tttggcagcc
     3061 gcgcggtatg cggtcagccc catagacgga atgcgagcag gccgacgccg agtacgatga
     3121 cgccggcgct aagactcttc aggatgcctt ccaggtgccc ggcatgcgca gcctccgccg
     3181 tgcggccgtt tgccatggcg cgcttcaggt ccttgtatgg ccgcgacgcc cggatttcca
     3241 ccaggctcaa aagcgccatc gccactgcga ccacgacggc cgatgcagag atcgcattgg
     3301 tctgcagcac gtagccggcg agcacgggca gcgctcccca ggagagcgca aaccaagcgt
     3361 cgcgatgaaa gcggccgcca aaccactcga ggttgtatgc caggagaaag aacccctccg
     3421 ggatcgcgat cagccaaagt agcggcgtgt cgcgcaccat gtaccagagg gcgatcgagt
     3481 acgcgcccac gagcgatacg atcgccaatc cccagagttg actccggctg aacatctcgc
     3541 cccacggtcg tgcgccgcgg ccgccgacgg catcgaccgc atgcgcgccg atgccgagcc
     3601 ccagaaaata gatcagcacg atcgctgcga cgcggtccca gtgaaccgtc ggtgcgagca
     3661 tggagccgat gacggtgaaa gataggacca tgccggtgta gggcagaaat agcaggccga
     3721 ccgctacgcg aaagcgctcg ggcccgaagg aaggaacgaa ccattcgcta cggcggtccg
     3781 acatgtaccc gcaccatatc atccgcgacg cgccggcaga gtcgagcacg ccgacaggaa
     3841 ctcacggtgc ggcggcggcg agacgtacgt agtgcttgaa cggcgtggca gcgccatcct
     3901 ccatcggtcg cggcgggaag cagcaggcgg atcgatccga cccgacccgg gaacggagtg
     3961 ctgccacgta tggggagata gacatcgagg tgatctcctg gctgtttcca gcttgcagaa
     4021 gctctttggc cgcgtcacga gccaatcgaa gcgcctatag ccgacaaaat cgacgacgag
     4081 ttttccagcg tagtcggcgg acacgcttgc ctcgcgacga atgacgagta ggagatcgtg
     4141 gaaaaatcgc ccatgaacgc gtccgaccgc ccctagatgg cgtcccaggc ccgaacgagg
     4201 caccccggcg cccgaatttc accgctcttt cggcgtttta tcgttacgtc aggccctgcc
     4261 cgatcgagga tcggccacat gcggtccgtc gatcgcgcca aaggagggct gacatgcgtt
     4321 ctatatttct tgtaatcgtg atctcagggt ggtgcatcgt ggcgaatgtg ccggcgtggg
     4381 cagcggacca ggtcctcatt cacgtgaacg ccgccgctat cgctggcggc gacggcagcg
     4441 caaaattccc ctacaacaac ctgcctgagg cgatcgcagc cgctcgcgcg gcctcaggag
     4501 cgccgttgat caaggtcgag cccggcgact accccctcgc tcagccgctc ttaatcgacc
     4561 gttcgatgga attgcgcggg tcgaccgagc aggtggcgag cggcgacagt tggcccagtg
     4621 gtgaagcggt tgctggcacg cagacgcggg tcttcgcgac ggatgcactc ggattccagc
     4681 cgctcgttat ggttggccga agcgacgctg tcgttatcag tggcgtacgg atcagcggtt
     4741 tcattttcga acaagccacc gcggtgggca tcggggtatt gctcaatcgc gtccaggatt
     4801 tttggatcgc cgagaacatc tttcgagggc cggcgttcat cggcatgcaa tccgtggcgt
     4861 cgtctggacg cgtgacggcc aatcacttca gcggcgccca ggccgccggt gcgtctctca
     4921 ccggcggcta ccctgaatca ccgtccaccg tggactttgt ggggaaccgc tcagtacgca
     4981 acaaccaggg gggcctgctg ctagatgggg cgagcatcaa catccccgag ctcggcgaca
     5041 agctcaacgc gacagtgcgc gataacgacc tcagcgacaa tgtctccaat ctgactgcct
     5101 tcggtctgcg cctgttcata ctgcgacggg atctcggcgc gccaggagat tcccaatcct
     5161 cgagccgcgt caatgcgctc gtgcaaggaa accgaatcta cgggaacgga gtcggcatcg
     5221 tcatcgacgc gggctttccc taccggcggg tcagtggtgt gtgcgattcc cgcgtgtact
     5281 caggccgcat cgatctcaaa cttgccggga acacgttgac ggccagcgta acgacaccgg
     5341 ccctcgtgac gttcactcga cccacggcgg cattgaatcc ttcatcgctg ccgcaatggc
     5401 agtacctgca cgccgccacg tttgcgattt ccgacaggga tggcacactg gcgcaggctt
     5461 ggatcgacca tccagaattc gatccgtttg tcggcggcct atgcccggca gacgtgacac
     5521 acgagcctct ggagaatgtc ctgatctaca acagcgccga gctgaagggc aggaacttct
     5581 agcgacgccg accaagtcac tgttctgtac cttatagcta cagcgtgacg acccgtgttc
     5641 cgtggtcgcc agcgcgtacc gtagcgtctg cttctgaccg acagcgaccg cagttatgtc
     5701 gctgcagatg tgcgggactt tcgcgggaat cggctgggct cggttgtgca tcgctgtata
     5761 gtggcgcaac gggcgccctc aggcagttga tgaaacacgc aacgctaacg aactacgaac
     5821 caggggtcgt gggttcaagt cccgccgggc gcgccagaag tctttcgtac ttcaacggcc
     5881 tgagacgatc acggcttcgg gcccttttgc ttcagcgggg acttttcgcc gaatcgcgga
     5941 tcagagaggg gagcaacatg ttgttcgcgt gcttctcgaa ggcgatatgg tggcaaggct
     6001 ggcgcaacgc gccacgttat gatccgcatc ggccgcaggt tacctgccac tctggtcagt
     6061 actggtcgag cgcataatct ccctagatgg cgtccgagac ccgaacgcgc cagcgccgaa
     6121 tttcaccgct ctttcggcgt tttctcgtta cgccgggtcc cgcccggtcg aggatcggcc
     6181 acatgcggtc cgttgaccgc gctaaaggag ggctgacatg cgttccatat ttcgtgcaat
     6241 cttgatcgca gcgtggtgca tggtggcgaa tgtgccggcg tgggcagcgg acaaggtccc
     6301 cgtggcgatc aagggttacg acccggtcgc ctacttcact gtgggcaagc cgaccaaagg
     6361 aagtccggac atcgcgctcg agtgggacga gcaccgttat ctcttctcca ccgaagaaaa
     6421 ccgtgcgcgc ttcaaggccg atccggcccg ctacgcgccg cagttctcgg actactgcgc
     6481 gatggcgttg agtaaaggag aactggtcga ggcgaacccc gagaactgg
//
LOCUS       DBNHXB010000338         2719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2942298, whole genome shotgun sequence.
ACCESSION   DBNHXB010000338 DBNHXB010000000
VERSION     DBNHXB010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2942298"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="AC162G_13700"
     CDS             <1..604
                     /locus_tag="AC162G_13700"
                     /inference="COORDINATES: protein motif:HMM:NF024644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5076905.1"
                     /translation="GVQGAFPALSQQFVPYNCMHCDEPPCVDVCPSQATYKRPADGIV
                     VVDQQACIGCGLCLPACPYHARFINPETGKADKCNACLQRVEQGEAPACVATCIGGAR
                     LFGDLNDPESEVAQALRAAKSVKRLPAVGVDTGPNIYYVNDDVDEKKLAVTAPQAPPA
                     DAFYKWVVIPGVLLAAGATFLGQAAAFARQLHKGESEFDE"
     gene            716..1495
                     /locus_tag="AC162G_13705"
     CDS             716..1495
                     /locus_tag="AC162G_13705"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HIR5076906.1"
                     /translation="MSAEVLTLAERKRLRAKKLKKHGLVNIVTHWFNAVSWLVLLLTG
                     LGILGSPSLRLMPQGYIDFMRELFGGIGGLVETHKVFGIIWLLVITYNVLLGFRRYFV
                     PFAAKRMLLTQDDVEWLKRKGLQLLGRKVTLPPQDAYNAGQKLYGYVVAIGTLTIGVT
                     GLIMTFSHIFPPEMRWIVQWSRPFHFGAVGMVVAGLIIHVYMAAFFPEEKSAFFSMFT
                     GEVDALYARLHHKKWYDDKMEEERGYEMHVRLEEIEARDQI"
     gene            1504..2532
                     /locus_tag="AC162G_13710"
     CDS             1504..2532
                     /locus_tag="AC162G_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HIR5076907.1"
                     /translation="MPSRRPSFFYHLHPPTIPERESRFRYTFGLGGISVLLFLVLIVT
                     GLLLTFFYIPTPGDAFRSIEMIAFQVPFGWLIRNLHYWAAQAAVLVVALHMLRVILTG
                     AYTAPRRANYLIGVALLAGTLLLDFTGYVLRWDEGTRWALVVGTNLVKEMPLVGSALY
                     QIIAGGAEIGAATTIRFYAWHILGLAVPSFILIGWHIFKVRRDGGIAHRPRAGDRAPR
                     IDRAQLVRTETIAMLVVVAALLGLSILLPAPLGPIADLPAASGKAQAPWFFLWVQALL
                     RSVPPLWAGILIPLGLLIALSLIPYLIDRRSEGAAEWFNRPGRVAQALILLMLAGLVG
                     LTLVEIAQ"
BASE COUNT          457 a          891 c          842 g          529 t
ORIGIN      
        1 gggcgtgcag ggcgcgtttc cggccctcag ccagcagttc gtgccgtaca actgcatgca
       61 ctgcgacgag ccgccgtgcg tcgacgtatg ccccagccag gcgacgtaca agcgtcccgc
      121 agacggcatc gtcgtcgtcg accaacaggc ttgcatcggc tgcgggttgt gcctgccggc
      181 atgtccttat cacgcgcgct tcatcaatcc cgaaaccggc aaagccgaca aatgcaacgc
      241 ctgcctgcag cgcgtcgagc agggcgaggc gccggcttgc gtggcgacgt gcatcggcgg
      301 ggcgcggctg ttcggcgacc tcaacgatcc agaaagcgaa gtcgcccagg cgctgcgcgc
      361 ggcgaagagc gtgaaacgcc tgccggcggt gggcgtcgat accgggccga acatttacta
      421 cgtcaacgac gatgtcgacg agaagaagct cgccgttaca gcgccacagg cgccgccggc
      481 agacgccttt tacaagtggg tcgtcatccc tggcgtgctg ctcgcggccg gcgctacgtt
      541 cctgggtcag gcggctgcct tcgcgcgaca actgcacaag ggcgagagtg agtttgacga
      601 atagttagag ttagagtgga gagttagagt gtgaatcgga tggaggcaat tcactctaac
      661 tctaactcca gactctaact ctaactccaa actctaactc tggaaggaga gacaaatgtc
      721 agccgaagtt ctcaccttag ccgagcgcaa gcgcctgcgg gcgaagaagc tcaagaagca
      781 cggcctggtc aacatcgtca cgcactggtt caacgccgtc tcgtggctcg tcctgctgct
      841 caccggcctg ggcattctgg gcagccccag cctgcgcctc atgccgcagg gctacatcga
      901 cttcatgcgg gaactgttcg gcggcatcgg cgggctggtc gagacgcaca aagtgttcgg
      961 gatcatctgg ctgctcgtga tcacctacaa cgtgctgttg ggcttccggc gctacttcgt
     1021 gcccttcgcc gcgaagcgca tgctgctcac ccaagacgac gtcgagtggc tgaagcggaa
     1081 gggtctgcaa ctgctcggac gcaaggtgac gctgcccccg caggacgcct acaatgccgg
     1141 ccagaaactg tacggttacg tcgtcgcgat cggcacgctg acgatcggcg tgaccgggct
     1201 gatcatgacc ttctcgcaca tcttcccgcc ggagatgcgc tggatcgtgc agtggtcgcg
     1261 gccgttccat ttcggcgcgg tcggcatggt cgtggccggg ctgatcatcc acgtgtacat
     1321 ggcggccttt ttccccgaag agaagtcggc gttcttctcg atgttcactg gcgaggtcga
     1381 tgcgctgtac gcccgcctcc accacaagaa gtggtacgac gacaagatgg aggaagagcg
     1441 cgggtacgag atgcacgtgc gcctggaaga gatcgaagcg cgcgatcaga tataatcgcg
     1501 tccatgccct cccgtcgccc cagcttcttt tatcacctcc atccgccgac gatccccgaa
     1561 cgcgaatcgc gttttcgtta caccttcggg ctgggcggca tttcggtcct cctcttcctc
     1621 gttctgatcg tcaccggcct gctattgact ttcttctaca tccctacccc cggcgacgcg
     1681 ttccggtcga tcgagatgat cgcctttcaa gtgcctttcg gctggctgat ccgcaacctg
     1741 cactactggg cggcgcaggc ggcggtgctc gtcgtcgcgc tgcacatgct gcgcgtgatc
     1801 ctcaccggcg cgtacacagc gccgaggcgc gccaactatc tcatcggcgt cgccttgctc
     1861 gccggcacgc tgctgctcga cttcacgggc tatgtgctgc gctgggacga gggcacgcgc
     1921 tgggcgctgg tcgtggggac gaatctcgtc aaagagatgc cgctggttgg tagcgcgctg
     1981 taccaaatca tcgccggcgg agccgagatc ggcgcggcga cgacgatccg cttctacgcc
     2041 tggcacatcc tcggcctggc cgtgccgtcg ttcatcctga tcggctggca catcttcaaa
     2101 gtccggcgcg atggaggcat cgcgcaccgg ccacgcgcag gcgaccgcgc gccgcgcatc
     2161 gatcgcgcgc aactggtcag gacggagacg atcgcgatgc tggtggtggt ggctgcgctg
     2221 cttgggctgt cgatcttgct ccctgcgccg ttaggcccca ttgccgacct gcccgcagcg
     2281 tccggcaagg ctcaggcgcc gtggttcttc ctgtgggtgc aggcgctgct gcgatccgtt
     2341 ccgccgctgt gggccggaat cctgattccg ctcgggctgc tgatcgcctt gagcctgatt
     2401 ccctacctta tcgatcgccg cagcgaagga gcggcggagt ggttcaaccg gccgggccgc
     2461 gtcgcgcagg cgctcatact cctgatgctc gccggtctcg tcggattgac tctcgtggaa
     2521 atcgcgcaat aaaccctgtg tctccgcgcc tccgtggctt gatctcccca tgaatgccga
     2581 tcgtcgtctc gtctttgccg cactcgccct ggccatcgcc gccgccttcg cgttcgcgcg
     2641 ccaggcaacg atgccaactg caaagtcgat cacgccgacg ctcaccggcc agccggagtt
     2701 gtgcctgacc tgccacgac
//
LOCUS       DBNHXB010000339        22565 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1936382, whole genome shotgun sequence.
ACCESSION   DBNHXB010000339 DBNHXB010000000
VERSION     DBNHXB010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 22565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22565
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1936382"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="AC162G_13715"
     CDS             complement(<1..372)
                     /locus_tag="AC162G_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5076908.1"
                     /translation="MEIRFWGAARTVTGSMHLLTIDGKRLLLDCGMYQGKRSEAYDRN
                     KHLPFDAASIDACILSHAHIDHSGNLPTLVKSGFRGNIYATPATRDLCSAMLLDSAHI
                     QESDVKFVNKRRAEKGEPPFKP"
     gene            complement(483..2381)
                     /locus_tag="AC162G_13720"
     CDS             complement(483..2381)
                     /locus_tag="AC162G_13720"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5076909.1"
                     /translation="MTGARILVVDDTAASRARLIERVLHPAGYTTAEAAGLDEARQRV
                     LAFEPDVVVTGWQTDRNDGLRLLREYGADIPFVVILAQRSLAAVEEALAAGAVDALAK
                     PLEDARFAASIERALRWKRLAHERDTLREQVERQAREFRALSGIGKTITSLLYVDEIL
                     AQVVSAAVNLTQADEGSLLLLDPDTGELYLRASKNMDESAARNLRVRVNDSLMGQVIR
                     TGQPVMVGGGEDVKVKTSFLVKSLLGAPMSVGGRVIGVLSVHHKLSSRSFSDHDMRLL
                     STLADYAAIAIENARLYWAAQGERERLHTILRDTQDAVIVTDPEMRIVLANSSARVAF
                     GLSEDVVGARLPDVIRSPALLDLFAQHKQSGHGWRAEVPLDDGRTMQAQLSEFKGIGF
                     GVVLQDISNLKELDRIKSDFISIVSHDLRTPLTTIRGYVELLPRVGPLNDMQQQFVER
                     IERSMVNIVDLIADLLDVSRIEAGLDKEMEPTDLAKAVREVSASLRSAAEAKRQVLSV
                     DVSPLPKILGNGRRLEQVVANLVGNAIKYTPEGGRIDVSLQEDGEFVVLRVRDTGIGI
                     TPEDQSRIFDKFYRVETDATLAIAGTGLGLSIVKSIVEKHKGRVWVDSEPGVGSVFTV
                     VLPKYAAP"
     gene            complement(2521..3612)
                     /locus_tag="AC162G_13725"
     CDS             complement(2521..3612)
                     /locus_tag="AC162G_13725"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HIR5076910.1"
                     /translation="MIDLNDAQAIRARDPEGMLRHIAGLPDQCEAAWRDAQAIELPES
                     FREARQVVIAGMGGSAIGGALFQSLAAPQMRIPVHVVRDYTLPAFAEGPSTLVIASSH
                     SGDTEETLAAFQEARARRCQLIAIAGGGRLEAMARQFGAPFMRVAYESMPRAALGYSF
                     VPLAAFASRLGWLDDPSAHPSTSLRASLREAAAVMREWNAELVPESPVVKNLAKRMAG
                     QMVGRFVIVYGAGLFAEVARRWKGQVNENGKQFAAFEALPEADHNAILGSSYPEGMAN
                     HLKVIFLTGVSDHPRNRARVEISRQVLMVQGCDTDVLTARGESLLAQMLSLIQLGDWI
                     SGYLGIANGVDPSDTELLLEFKQRMAELG"
     gene            complement(3609..4499)
                     /gene="rsmI"
                     /locus_tag="AC162G_13730"
     CDS             complement(3609..4499)
                     /gene="rsmI"
                     /locus_tag="AC162G_13730"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006194123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR5076911.1"
                     /translation="MGQHMGTLYIVGTPIGNLGDMTFRALETLRAVSLIVAEDTRRAR
                     VLLNHFDIDKPTLSLFEHNEAARVERVLRALDAGDVALISEAGMPGLSDPGYDLIRAA
                     LAHGVTVTPIPGPTAAIAALVASGLPTDAFVFLGFLPRRAADRRRLLRDVSRERRTLV
                     AYEAPHRLAEALADIQHVLGDRAIAVARELTKRHEEIWRGAVSGARARFTGEVLGEVT
                     LVVAGAPEPQPWSEPDVRAAVAQALRDGASISDAARDVAVVSGWLRREVYHIALRLSQ
                     PSNSNSNSNSDSNSLGRSKR"
     gene            complement(4558..5307)
                     /locus_tag="AC162G_13735"
     CDS             complement(4558..5307)
                     /locus_tag="AC162G_13735"
                     /inference="COORDINATES: protein motif:HMM:NF018455.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAD55 family ATPase"
                     /protein_id="HIR5076912.1"
                     /translation="MATNNNDRVATGIAGLDDMLSGGFLRESAVLLRGAPGTGKTTLA
                     LQYLVHGARNGEPGLLISFEEFPQSLHRDAQSLGWDLKALEDKGLLHLHFTSPLVLLE
                     SLRSPTSALSRLLLEGGIRRVVLDTVTHFTRVTNDPVELRKVYNTLINALKREGATAL
                     LVGEDTRFESQRFEQGRLSYIVDAIVLLRYVEIDSAVQRAMVIIKMRGSDHAKEIRRF
                     EINEKDGVVVTGVFEGREGILSGISHRIVAK"
     gene            complement(5368..6054)
                     /locus_tag="AC162G_13740"
     CDS             complement(5368..6054)
                     /locus_tag="AC162G_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076913.1"
                     /translation="MPDSRARLYNTLTLALLALSAVVCAAYAVLFVAAPRSIAALPTG
                     TLGPIGTPTVAPTPSRTPRPTNTSTHTPGPSDTPTATPVITVTLTGRETRTRTPTPGP
                     SHTPSPTLSPFNYVASVEYQRSIYGQNWAGIAGLVYGLDSKHQTNILVRAWGDPPLGP
                     DGLEIPSGIAVQYGVSGFEITLGDKPVIGRWNVQLIADDGEPLSDVIPVDMNSDPRLN
                     LAYIVFVQNH"
     gene            complement(6057..6761)
                     /locus_tag="AC162G_13745"
     CDS             complement(6057..6761)
                     /locus_tag="AC162G_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076914.1"
                     /translation="MSEYTARLLNQLASLLLGLSGLAIFIFAVIFLVNPFGPRAIASN
                     ASAITLTPSPTRPATWTPTPTPTPTDTPGPSPTPSDTPTPSPTRRPVATRTFTPSPKP
                     TGPTFSPFKYTKTNDEIRFARDPYGAPCGSWLGVGGEVLDVDGSPLPGVTIVGWGGPV
                     PEQEKRVFVSGSSSRINGLYGSPAAYEIYIGAPGEFDFILQVYQNGQPASDLIRLRTS
                     SDCRADLAIVNFQRNH"
     gene            complement(6910..7578)
                     /locus_tag="AC162G_13750"
     CDS             complement(6910..7578)
                     /locus_tag="AC162G_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076915.1"
                     /translation="MRGAQTFNTLTMVFLGFTVVIAAAYAIIFVAGPIGGPLTPEIVV
                     VTPTPIGTPTVTRPPTWTPTVPPSPTSTRPTPTHTPTRTPRPTRTPLPTDTPTPSITP
                     TPTEDVCKTLELLGPPPGQKFFQYDVPVLVWTFGRPLQPNEHFDLLLDPPGAGEGSIA
                     WADEADPKNKNCNPFCEYQVGLNGVYSGGRFQWTVAIIRAGADRRVLGTVCSAPEPYF
                     FIWP"
     gene            complement(7687..8643)
                     /locus_tag="AC162G_13755"
     CDS             complement(7687..8643)
                     /locus_tag="AC162G_13755"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5076916.1"
                     /translation="MTIRRCAYQVLSAALIGLLVACDAGASAPAATNATEPPTAPAQI
                     VPTSPPAPTEPAPTEAPAIARVNGEPIALSSYENQVAQFEAALIAQGVDPNSADGQAQ
                     LAAVREQVLNGMIDQLLLEQEARRKGISVSDADLEAAMQEIISASGGPDAFAAQLAAL
                     GQTEAEFRQGQRAAMLATIIRDQVIAEVSSVAPQTHARHILVDNAELANSLLAQLQAG
                     ADFAALAQQYSQDTLTRDGGGDLGWFPRGALISKEVEDAAFALQPGQYSGVVQSAFGF
                     HIVQVIEADAARALTPEQLLNLQQVYFERWMSGLRSSANIER"
     gene            8980..12132
                     /locus_tag="AC162G_13760"
     CDS             8980..12132
                     /locus_tag="AC162G_13760"
                     /inference="COORDINATES: protein motif:HMM:NF027484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HIR5076917.1"
                     /translation="MLDNANPMPEPPSNPLPPRLARRLSELPSHRRSLILYGVIAVLV
                     VAGLLLPPISLFDRMFKPCGELAIGGDSQSAQHPNGLTVLPASAEQAYRLTLSAISQA
                     DFKSGAAGETMIAALDALPVNLMLVSPVYQIEACGDEPQPATLSVAVPPGAQPETTLD
                     LYAWDSEERQWSWIGSSHDLTNGAVLASTDALPEAVALMQTTSVSPAVGAEVTPGDKI
                     SGDVSRIVGEVYPTGLVIAPDGSLGGDRSSLPEAASPAQAVYPVVRNYLVGGRPNPDL
                     VRGVLDVAEARQTHIDALTALAVEDDYAGVMLDYQGLVETDREAYADFVSDLASALHA
                     QGRQLAVVLPEPLFTPEGMADTTGYDWARIGRAADMVLSPMDDAAIVAWTTTQVDRYK
                     FQPILSALSVQSVDGSETPFTFAEAIEMLGAVTAGEAVSLTAETPVTFTLQAKFPIDN
                     FAYNPAAGIYTYEYVDKSNATHRVVVTTAAALAKALAGVLPLHTRGAALRGATGDTSP
                     RLSEVLRAYRQQATPKLESEIEILWQIQPEKGESSTVSKPLSESWLAWRAPAEPGRYK
                     VSATIAGVDLGSVDVAVSAGSPEEEVAAVTAGVGSGGGQATSQSGAAAGAEAGGCYGA
                     AYVADVTVPDGTRFDKGKEFTKTWKVRNNGTCAWTADTELAFISGSQLSAPSTVKVGA
                     LEPGREIEISVSMKTSDADGNFTGVWQLRNKDGFYGDSLTVVIAAGEQTTVAPPVAPV
                     GNIGSFEAGAHIDGFRRPDLMKYAGLKWVKIQTYAGVDVSGAIDNAHAQGFKILLGIV
                     GDKERVMDGVYQDEYAAAVAAMAAAGADAIEVWNEPNINREWPTGQVNGANYTALLAK
                     AYRAIKAANPGTLVVSGALAPTGFWGAAGCSPDGCNDDTFLAQMAAAGAASYMDCIGA
                     HHNAGATPPSASAGHPGGSHYSWYFLPTLNLYYNSFGGARKVCFTELGYLSDDGYPSL
                     ESTAPAFAWASNVTVGQQAAWLAEAAAMSARSGRVRLMIIWNADFTRYDSDPMAGYAM
                     IRPDGSCPACDALRNVLGGR"
     gene            12195..15344
                     /locus_tag="AC162G_13765"
     CDS             12195..15344
                     /locus_tag="AC162G_13765"
                     /inference="COORDINATES: protein motif:HMM:NF027484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HIR5076918.1"
                     /translation="MQSSIQRILIPLVAIGVIAVVGIVFLLASQATVPACGAFVVTAG
                     APVTLDGGASVALIAGSGELRFDSAALTEADLTDEVNAQALAAVNAMPVNLRAEGLIL
                     TFTRCNEEPAPVHLESPAPPSAALRPPQTAGEGRWQAGAALDAYAWDGTQWAWLAGAA
                     DQLSVDLPDLPKILAWVETLPTAPVIGTEPDPRTGGLAPEYYGVITELYAPGLTVAAN
                     GSVNGQVPAAPPEGAPYVVYPLVTNVANGQADATSVEAMLNDEAARQRHVEALASIAN
                     GANFAGIAIDYQGWTPQARDVFASLMEDLASALHAQGKQLFVVLPGKSAAGGPYDTDP
                     DEYDWARIGRAADVAHVLLPFQAGVYPPPPEGDADSLLNRIPTLIDRNKMQAVLSAAS
                     ARTSFGSLTSAPFSDTLDALISRTPSTISSTVGVSLPLSLNVYFDPNTGGYSNGVIDG
                     QIIAFTAATLASKLNALSGLGLRGAVIRDIQGPDVAPNLFEPIKAYRQQSAISGSSEL
                     SVDWTITAADGSVVLSESRSLASADVVWTPAGDGVYTATVTAGSVTKDVVVVTVGEEE
                     TETGAGGGGKDTPSTPEAPCLNAAYVADVTVPDNTRFDKGKDFTKTWKVRNSGTCAWS
                     ADTELAFVRGARLGASSPVKVGALEPGKTIDVSVPMESGDEDGAFTGIWRLRNEDGAF
                     GDELSVVIKVGAEVVAPPPIAPPAGGGPTQYGIHSHYYGYIDTEAGAQSIVNYTNELG
                     LGWTKIQFRWGDYDYYCGGPDLNRLNTMISAANGAGLKVLLSIVTSPPCTHPWTGEVH
                     APPDDPAALAELAGGLADLFKGRIHAIEVWNEQNISREWLTSPQKLDAARYTQLLAAA
                     YNAIKAKDPNILVISGALAPTGWNDGVNATDDFEYLRQMVAAGAAKYMDCVGTHVNAL
                     RVPPSASLGGPYDNLFSPPHHSWYFKDTVLGYQSITGKPACVTEFGVASSETIGQVEG
                     FEWASENTQQEQADWVTEGMSLCKQWGCRLVILWNLDYGPVTRLVTDNALYSFIDIGL
                     GRRPVFHAVKSWCAANGCR"
     gene            15348..16685
                     /locus_tag="AC162G_13770"
     CDS             15348..16685
                     /locus_tag="AC162G_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076919.1"
                     /translation="MNQDRRSIWDRIPTPALIVLWLLAIAFGAVMVFLAFFNPAAPQE
                     MPVSTPTAVSVVGSVATATAPATQPVEPAAPASNPTPQPTIAPLHDPSMEFGIQSDAL
                     TSDPAYAIGLLRDKLLLGWVKQQLRWGNFSPAPGEMDWSGFDRVVEAASTKDIRVLLS
                     IVTAPEWSRPGVEGVEGPPNDLNEYARFVGEVVDRYQGKVQAFEIWNEQNIDSEWRTN
                     PQAVSPEQYVEMLKLAYQAIKARDPDAMVISGALSPTGAFGGGCDARGCDDQPYLKRM
                     VELGFLNYADCVGVHVNGYNLAPQYRHDEGYNDPTASFRGPFDNPHPSWSFRSTLEDY
                     YNIIGKQKPLCVTEFGWASYEGISDTALPSYEFGLDNTAEEQAQWDADAFRLMQEWGF
                     VKLAILFNLDFAQKSPLGAQDRTAPWSITDLQGGPRPAFDAVRDFMKGVLGIP"
     gene            16807..17964
                     /locus_tag="AC162G_13775"
     CDS             16807..17964
                     /locus_tag="AC162G_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076920.1"
                     /translation="MAFSSVRFRCILTLIAGLLMTGCASATAVTPTPVPTPTSWPRAR
                     LKSPEYGIQMFAWWKPEIAGRDLNLIRDMGFTWVKQSFAWRDIEGLGKGQYAWKVADD
                     LVRRATKRHLKIIARVDRQPFWAQAPGTEPLENAPPANLQDFADFCYALADRYRGRIH
                     AYQVWNEPNLSREWGGQPPDPAGYVELLKACYVAIKRADPNALVISAGMAPTGTWTEG
                     IMPDAMFVEKMYEAGAAPYFDLLGAHAPGFKAPPEASPDEVAANPELGGQRFFSFRHV
                     EDLREIMVRHGDAGKQVAVLEMGWTTDPIHPEYAWHAVDEQTQADYLVRAFDFARQNW
                     SPWISIMTVIGLADPVWTPDDEQYWWTVVYPDWPETRVKPAYEALKAMEKK"
     gene            18037..18558
                     /locus_tag="AC162G_13780"
     CDS             18037..18558
                     /locus_tag="AC162G_13780"
                     /inference="COORDINATES: protein motif:HMM:NF017262.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HIR5076921.1"
                     /translation="MLTLNAPGLLALSFFLHLIATVVWIGGLVTMALVVWPGLRRALG
                     DDDRRLARAVEALDRRFKPLANLSLVVLILTGLVQMNANENYKGFLNFDNLWAQAILL
                     KHAAIIGMIAVGALLQFGVQPALQRNAMLAAAGVSNEEEVAALRQRMARLGRINLILG
                     VVVLILTAIARVQ"
     gene            18639..19613
                     /locus_tag="AC162G_13785"
     CDS             18639..19613
                     /locus_tag="AC162G_13785"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity
                     producing 5'-phosphomonoesters, hydrolytic mechanism
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HIR5076922.1"
                     /translation="MFEIVFLGTSAAAPSIRRNLSAHVVLHKEYRFLVDCGEGTQRQL
                     LKSGLGFKRLNRILLTHSHLDHILGLGGLLSTFMRWETIEEVEIFGGRSTLDRVEDLI
                     YRVVLRGARPPMRLSFVDVKPGRLLEDDSFELIAFPVMHRGGECFGYLFREKPRRPFL
                     VDKAEALGVPPGPERRRLVAGESVTLSDGRTIHPDEVLGPVIRGAALAHVGDAGRTSD
                     LIEHVRGADALVIESTYLDAEADLARSYDHLTAAQAARLAQDAQVGQLILTHISRRYG
                     QKQVLAEAQAIFPNTAVARDFDRFRVLKDKSMLLENLIVEEENWHVAA"
     gene            19600..19890
                     /locus_tag="AC162G_13790"
     CDS             19600..19890
                     /locus_tag="AC162G_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076923.1"
                     /translation="MSLRKAAVLDLASAAYEVDAEVKRGILKKADGHWTVADQEMSEW
                     LDRFEGHEVVVIVASMSDEKPIEPRICTRCGYEFVGVECPRCRAARIRLRGE"
     gene            complement(20115..21584)
                     /locus_tag="AC162G_13795"
     CDS             complement(20115..21584)
                     /locus_tag="AC162G_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076924.1"
                     /translation="MRKVAFSSAALIIALSFVRVTFALIVRASQQALDLASRDKITVS
                     VTTRPNVIASIFVRSNPPAGQAMASSQKTLLAMTPEQLHGSSLSAELPSAPPNSVDIT
                     GRSTGYTEVGYEFTATVRPKSTTRPVTFTWQVSDLGSFTRIVTDSLYDTQTFTWAVPG
                     NKIITVVAGNGDKTVDDVHIVNIQPQPLDDIAINGTQAGTINTAYNFTAIASPPNAYK
                     PITYTWSPPPNSGQGPVVTYTWTTTGIKIFSVVATNAANNAVTGTHVVAIDFSPKKVY
                     LPSVLRDACFGLTLFPIENDDNDGSYVVRWCASGAEYYVLEETVFGSISPMVVYSDSD
                     TYAPLSDRGPTRYYYRVKSISSSGSNRPSNEESVDVLWELEPNNYIDGPNAPDESNGP
                     LLSGLWYYGKPGPVDDKWDCFRFFATSAGLITVDLENHTGRQIHLALFFQIENPDPTQ
                     DTTPPYHIEHFGAAGRYYACIYSENGDPDAPYRLKATFP"
     gene            22311..>22565
                     /locus_tag="AC162G_13800"
     CDS             22311..>22565
                     /locus_tag="AC162G_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076925.1"
                     /translation="MRRSLKVYGCLSLAAKCPSDRFQVIQHLASRLIAAARIGVQGFL
                     QRGDKLLRHAILDKKRFGKSIQHRCMVSRAVGKQFFQNLPR"
BASE COUNT         4107 a         7006 c         7402 g         4050 t
ORIGIN      
        1 cggtttgaac ggcggctcgc ccttctcggc gcggcgcttg ttgacgaact tcacgtcgct
       61 ctcttggatg tgcgccgaat cgagcagcat agctgagcac agatcgcgcg tcgccggcgt
      121 ggcgtaaatg ttgccgcgaa agccgctttt caccaaagtc ggcaggttgc ccgaatggtc
      181 gatgtgcgcg tgcgacagga tgcaggcgtc gatcgaagcg gcgtcgaacg gcaggtgctt
      241 gtttcggtcg taagcctcgc tgcgcttgcc ctgatacatc ccgcagtcca gcagcaggcg
      301 cttgccgtcg atcgtcagca agtgcatgga gccggtgacg gtgcgcgcgg ctccccagaa
      361 tcgaatctcc atagaccctc cgatctcatt ggcggcaagg caagaatcct tccgacttcc
      421 gaagtctccg agacttcgga agtctgctgg ttctgcgtta taccctctct tcgcgccggg
      481 cttcaaggcg cagcgtactt cggcagcacg acggtgaaga cactgcccac gcccggttcg
      541 ctatccaccc acacgcggcc tttgtgcttc tcgacgattg acttgacgat cgacagtccc
      601 aatccggtgc cggcgatcgc cagcgtcgca tcggtctcaa cccggtagaa cttatcgaaa
      661 atacgtgact ggtcttccgg cgtaatgccg ataccggtgt cgcgcacgcg gagcacgaca
      721 aattcgccat cttcctgcaa cgagacgtcg atccggccgc cttcgggcgt gtatttgatg
      781 gcgttgccga ccaggttcgc gacgacctgc tcgagtctcc ggccgttgcc gagaattttc
      841 ggcagaggcg acacgtctac gctcaatacc tggcgcttgg cctcggcggc ggatcgcagc
      901 gaggctgaca cctctcgcac cgccttcgcc aaatcggtcg gctccatttc cttgtccaga
      961 ccggcttcga tgcggctgac gtcgagcagg tccgcgatca ggtccacgat gttgaccatg
     1021 ctccgctcga tgcgctccac gaattgctgc tgcatgtcgt tgagaggccc tacgcgcggc
     1081 agcagctcga cgtagccacg gatggtggtg agcggcgtgc gcagatcgtg cgacacgatc
     1141 gagatgaaat cgctcttgat tcgatccagc tccttcaagt tcgaaatatc ctgcagcacc
     1201 acgccgaagc cgatgccttt gaattccgag agctgcgcct gcatggtgcg cccgtcgtcg
     1261 agcggcacct cggcccgcca gccgtggccg ctctgcttgt gctgagcgaa cagatccagg
     1321 agcgccgggc tgcggatcac atcgggcagc cgtgcgccga ccacgtcctc gctcagaccg
     1381 aacgccacgc gcgctgagct gttcgccagc acgatgcgca tctcaggatc ggtgacgatc
     1441 accgcatcct gcgtgtcgcg cagaatagtg tgcaggcgct ctcgttcgcc ctgcgccgcc
     1501 cagtacaggc gcgcattctc gatcgcgatc gcggcgtaat cggccagggt cgacagcagg
     1561 cgcatgtcgt gatccgaaaa agaccggctg gacagcttgt gatgcacgct gagcacaccg
     1621 atcacgcgcc cgccgacgct catcggtgca ccgagcaggg acttgaccag gaacgacgtc
     1681 ttgaccttga cgtcctctcc gccgcctacc atcacgggct ggccggtccg gatcacctgg
     1741 cccatcagcg agtcattcac ccggacgcgc aaattgcggg cggccgactc gtccatgttc
     1801 ttgctggcgc gcaggtacaa ctcgccggtg tccgggtcga gcaagagcag cgacccctcg
     1861 tcggcctggg tcaggttgac cgccgccgac accacctggg ccagaatctc gtcgacgtat
     1921 aacagtgacg taatggtctt gccgatgccg gatagcgccc tgaattcgcg tgcctggcgc
     1981 tcgacctgct cgcggagtgt atcgcgctcg tgcgccaggc gcttccagcg cagcgcccgc
     2041 tcgattgacg cggcgaaccg agcgtcctcc agcggcttcg ccaacgcgtc gacagcgccg
     2101 gccgccagtg cctcttccac ggcggcgagt gagcgctgcg caaggatgac cacgaagggg
     2161 atatccgcgc cgtactcacg cagcaagcgc agcccgtcgt ttcgatccgt ttgccagccc
     2221 gtcacgacga cgtccggctc gaacgccagg acgcgctgcc gggcttcgtc gaggccggca
     2281 gcttcggccg tcgtgtagcc ggcgggatgc aggacgcgct cgatcagccg cgcgcgcgac
     2341 gcagcggtgt cgtcgacgac gagaatcctt gcaccggtca tctatagcct ctgccgaaaa
     2401 tcaggcgcct cggcgcgtgt agagtggatg gcgtgacatg tctcaatgac gttatcataa
     2461 ccgagatctt cttgatctgg caaaccgatt tggctccgat acccttgcgg ttgggccaat
     2521 tcaccccagc tccgccatcc gctgcttgaa ctccaggagc aactcggtgt cagagggatc
     2581 gacgccgttg gcgatgccca gataaccgga gatccagtcg cccagttgga tcagcgacag
     2641 catttgtgcc agaaggctct cgccgcgcgc ggtgagcacg tccgtgtcgc agccctgcac
     2701 catcagcacc tggcgcgaaa tctcgacgcg cgctcggttg cgtggatggt cgcttacgcc
     2761 ggtgaggaaa atcaccttca ggtggttggc cataccttca ggataggagc tgcccagtat
     2821 ggcattgtgg tcggcctcgg ggagcgcctc gaacgcggcg aattgcttgc cgttctcgtt
     2881 gacctgccct ttccatcggc gcgccacctc ggcgaagaga cccgcgccgt acacgatgac
     2941 gaagcggccc accatctgcc cggccatgcg cttcgccagg ttcttcacga ccggcgactc
     3001 cggaacaagc tctgcgttcc actcgcgcat gaccgccgcc gcctctctca ggcttgccct
     3061 gagcgaagtc gaagggtgcg ccgatgggtc gtccagccat cccagtcgcg acgcgaacgc
     3121 cgccagcggc acgaacgagt agcccagcgc cgcgcggggc atcgactcgt acgccacgcg
     3181 catgaacggc gcgccgaatt gccgcgccat cgcttcgagc cggccgcccc ctgcgatggc
     3241 tatcagttgg cagcgccgcg cgcgcgcctc ctgaaaagcc gcaagcgtct cttctgtatc
     3301 gcccgagtgc gacgacgcga tcaccagcgt cgacgggcct tcggcgaacg ccggcagggt
     3361 gtaatcgcgc acgacgtgaa cggggatgcg catctgcggc gcggccagcg attggaagag
     3421 cgcgccgccg attgccgatc cgcccatgcc ggcgatcacg acctgcctgg cctctctaaa
     3481 ggattccggc agctctatcg cctgcgcatc gcgccaggct gcctcgcatt gatcgggcag
     3541 gccggcgatg tgccgcagca tgccctcggg atcgcgcgct cgaatggcct gagcgtcatt
     3601 cagatcgatc atcgtttgct ccgtccgagt gagttagagt cagagttaga gttagagtta
     3661 gagttagagg gttgggatag gcgtagggcg atgtggtaca cttcacgacg gagccaaccg
     3721 gagacgacgg cgacgtcgcg cgcggcatcg gatatgcttg ccccgtcacg cagcgcctgc
     3781 gccactgccg cccgaacatc cggctcgctc cagggctgcg gctcgggtgc gccggcgacg
     3841 accagggtca cctctccgag cacctcgcct gtgaagcgcg cgcgcgcgcc gctcaccgcg
     3901 ccgcgccaaa tctcctcgtg ccgcttggtc agctcgcgcg caacggcaat ggcgcgatcg
     3961 cccaagacgt gctgaatgtc ggccagcgct tcggccagcc ggtgcggcgc ctcgtacgcg
     4021 accagcgtgc gccgttcgcg cgaaacgtcc cgcagcaggc gccgccggtc ggccgctcgg
     4081 cgcggcaaga agccgagaaa cacgaacgca tctgtcggca ggcccgatgc gaccagcgcg
     4141 gcgatcgccg ctgtcggacc agggattggc gtgaccgtca cgccgtgcgc gagcgcggcg
     4201 cggatcagat cgtaaccagg gtcggataag ccgggcatgc ctgcctcgga aatcagcgcc
     4261 acgtcgcccg cgtcgagcgc gcgcaggacg cgctcgacgc gagccgcttc attgtgctcg
     4321 aacaggctga gcgtcggctt gtcgatgtcg aagtgattga gcagcacgcg ggcgcggcgt
     4381 gtgtcctctg cgacgatcaa cgaaaccgcg cgcagggtct ccagcgcgcg gaaggtcatg
     4441 tcgcccaggt tgccgatcgg cgtgccgacg atgtacagcg tgcccatgtg ctgacccacc
     4501 cccgcccctg gcccctcccc cgtcgtcctt ccgacggggg aggggttggg gtgggggcta
     4561 cttcgctacg atgcgatgcg aaatgccgct gaggatgcct tcccgcccct cgaacacgcc
     4621 ggtgacgacc acgccgtcct tttcgttgat ctcgaagcgg cggatttctt tggcgtgatc
     4681 gctcccgcgc attttgatga tgaccatcgc ccgctgcacg gcgctgtcga tctcgacgta
     4741 gcgcagcagc acgatggcgt cgacgatgta cgacagccgg ccttgctcga aacgctggct
     4801 ctcgaagcgg gtatcttcgc cgacgagcag agccgtggcg ccctcgcgct tgagcgcgtt
     4861 gatcagcgtg ttgtacacct tgcgcaactc gaccgggtcg ttcgtgacgc gggtgaagtg
     4921 cgtgacggta tccagcacga cgcggcggat accgccttcg agcagcaggc ggctgagcgc
     4981 gctggtgggc gagcgcaagc tttcgagcag caccagcggg gacgtgaagt gcagatgcag
     5041 cagccctttg tcttcgagcg ccttcaggtc ccagcccagc gactgagcat cccggtgcag
     5101 cgactggggg aactcctcaa aggaaatcag caaacccggc tcgccgttgc gcgcgccgtg
     5161 caccaggtac tgcagcgcca gggtcgtctt gcccgtgccg ggcgcgccgc gcagcagcac
     5221 cgccgactcc cgcaggaatc caccggaaag catgtcgtcc agcccggcga tgcccgtcgc
     5281 cactcgatcg ttattgtttg tcgccatgtg tgtctcccta ttgcttcgat cgccctcgtg
     5341 actgctcggt tcttgccacg gaggacatca gtgattctgc acaaagacga tgtatgccag
     5401 gttcaggcgc ggatcgctgt tcatatcgac cgggatcacg tctgacaatg gctcgccgtc
     5461 gtcggcgatc aattgcacgt tccatctgcc gatgacgggt ttatcgccca gcgtaatctc
     5521 gaagcccgat acgccgtatt gaacggcgat tcctgaggga atctccagcc cgtccgggcc
     5581 aaggggcgga tcgccccagg cgcgcaccag gatgttggtc tgatgtttgc tgtccaaccc
     5641 gtagaccagg ccggcgatgc cggcccaatt ttggccgtag atcgagcgct gatactcgac
     5701 agaggcgacg tagttgaagg gcgacagcgt cggcgagggc gtgtgcgacg ggccgggcgt
     5761 cggcgtgcgc gtccgcgtct cacgtcccgt tagtgtcacc gtaatgactg gcgttgccgt
     5821 gggggtatcg ctggggcctg gcgtgtgcgt cgacgtgttc gtcgggcgtg gcgtgcggct
     5881 cggcgtcggc gcgacggtag gtgtgccgat cgggccgagt gtacccgttg gcagcgcggc
     5941 gatacttcgc ggcgcggcca cgaacaaaac ggcataggcc gcgcacacga cggcggaaag
     6001 ggcgagcagc gccagcgtaa gggtgttata gagtcgtgcg cgtgagtcgg gcatggtcag
     6061 tgattacgct gaaagttgac gatggcgaga tcggcgcggc agtccgaaga cgtgcgcagg
     6121 cgaatgaggt cggacgcggg ctggccgttc tggtagacct gaagaatgaa gtcgaactcg
     6181 cccggcgcgc cgatgtagat ctcgtaagca gccggcgaac cgtatagtcc gttgatgcgg
     6241 ctgctgctgc cggacacgaa tacgcgcttt tcttgctccg gcacgggacc gccccagccg
     6301 acgatagtca caccagggag cggcgagccg tcgacgtcga gcacctctcc acccaccccc
     6361 agccatgaac cgcatggcgc gccgtacgga tcgcgagcga agcggatctc gtcgttcgtc
     6421 ttagtgtact tgaacggcga gaaggttggc cccgtgggct tcggcgacgg cgtgaaagtg
     6481 cgggtcgcca ccggccggcg cgtcgggctg ggcgtcggtg tatcagacgg cgtcggcgaa
     6541 ggtccaggcg tatcagtcgg cgtcggcgtc ggcgtcggcg tccaggtggc cggccgcgtc
     6601 gggctgggcg tcagcgtgat agccgacgca ttcgacgcga tcgcccgcgg gccgaacgga
     6661 ttgacaagga agatgaccgc aaagatgaat atcgcaagcc cgcttaaacc cagcagcagc
     6721 gacgcgagct gattaagcag acgggctgtg tattctgaca tgcgtccggt tccccaatcg
     6781 cccaccctcc cgcaggctgc ctgcgccccg cccagcgggg ttgtgctcga taacattttc
     6841 gcgcatagga tatgcacaga gcgtgctgtt tttgacccaa aaagcgtcaa acctgcggga
     6901 gggtagcagc tagggccata tgaagaagta aggctcaggc gctgagcaca ccgtccccaa
     6961 caccctgcgg tccgcgcccg cacggataat agcgaccgtc cactggaagc ggccgccgct
     7021 gtagacgccg ttcaatccga cctggtactc gcagaacggg ttgcagttct tgttcttcgg
     7081 atcggcttcg tcggcccagg cgatcgatcc ctcgcctgca cccgggggat cgagcaggag
     7141 gtcgaagtgc tcgttcggct gaagcgggcg gccgaacgtc caaaccagga ccggcacatc
     7201 gtactggaag aacttctggc cgggcggcgg gccaagcagc tcgagcgtct tgcaaacgtc
     7261 ttcggtgggc gtcggcgtaa tggaaggcgt cggtgtgtcc gtaggcagtg gtgtccgggt
     7321 gggacgcggt gtgcgcgtcg gcgtgtgcgt cggcgtcggt cgcgtcgacg tggggctagg
     7381 cgggacggtc ggcgtccacg tcggcgggcg ggtgacggtg ggcgtgccga tcggtgtcgg
     7441 cgtgacgacg acaatctccg gcgtgagcgg ccccccgatc gggccggcga cgaagatgat
     7501 ggcatacgcc gccgcaatca cgacggtgaa gcccaggaag accatggtca gggtgttgaa
     7561 tgtctgtgcg ccgcgcacgt gtgtcactct cagggacgca acggaacacc gggactgtcg
     7621 gcgacggcag gcccctcggc atcgctcagg acaggctcag cgcgcaagag gtgcgccgtc
     7681 tcatctctac cgctcgatat tcgccgacga ccgcaggccg ctcatccagc gttcgaaata
     7741 aacctgctgc aaattgagca gttgctcggg agtcagggca cgcgccgcat ccgcctcgat
     7801 gacttgcacg atatggaagc cgaaggcgct ctgcacgacg ccgctatatt ggccgggctg
     7861 caaggcgaag gccgcgtctt cgacttcctt ggagatcagc gcgccgcgcg ggaaccagcc
     7921 cagatcgccg ccgccgtccc gcgtgagggt atcctgcgag tactgctgcg ccagcgccgc
     7981 gaagtcggca ccagcctgca actgagccag gagcgagttc gccagttctg cgttgtcgac
     8041 caggatgtgc cgcgcgtgcg tctgcggcgc gacgctcgac acctcggcga tcacctggtc
     8101 gcggatgatc gtcgcgagca tcgccgcgcg ctgtccctgc cggaactcag cttcggtctg
     8161 tccgagcgca gccaactggg cggcgaaggc atccggcccg ccgcttgcgg aaatgatctc
     8221 ttgcatggcc gcttctagat cggcgtccga tacgctgatg cccttgcgtc gcgcttcctg
     8281 ttccaggagc agttgatcga tcatgccgtt gagcacctgt tcgcgcaccg ctgcgagctg
     8341 cgcctgcccg tcggccgaat tcggatcgac gccctgagca atcagcgccg cctcgaactg
     8401 tgcaacctgg ttttcgtaac tgctcagcgc gatcggctcg ccgttgacgc gtgcgatggc
     8461 cggcgcctcc gtgggcgctg gctcggtcgg cgcagggggc gacgtgggca cgatctgcgc
     8521 cggcgcggtc ggcggctcgg tggcgttggt cgccgcaggc gcggatgccc cggcgtcgca
     8581 cgccacgaga agccctatca aagcggcgct caatacctgg taggcacaac ggcgaattgt
     8641 cacacgttcc tccagatgga cgatagcgtc atccgcacag acagaaaccg cggctattat
     8701 acatacccgg tgtggtttgc gcaaacaacc gtttggtaat agtggcctca ccccccgccc
     8761 ccggcccctc tcctaaaaag gggggtgggg ccaggggtgg gggaggttcc ttgcacgaaa
     8821 tacccggcca tgctagaatc gcgccaaccc cttccggccg ctgactcgag gagaattgct
     8881 gcattgcacc ggcgactatc ccgcctgcca ccggcacgga tcgactgtga cggcgcaatc
     8941 cgccgtcctt tcgtgtcagt caacgggtga gaggtggtca tgcttgataa cgcgaacccc
     9001 atgcccgagc cgccgtctaa tccgctgccg ccgcgattgg cgcgccggct gagcgagttg
     9061 ccttcgcaca gacgcagcct gatcctctac ggcgtgatcg ccgtcctggt ggtcgccggc
     9121 ctcctgctgc cgccgatctc gctgttcgac cgcatgttca agccgtgtgg cgagctagcc
     9181 atcggcggcg attcgcaatc cgcccaacat cccaatggcc tgaccgtcct gccggccagc
     9241 gctgaacagg cgtatcgcct gacgttgagc gccataagcc aggccgactt caaatccgga
     9301 gcagccgggg agaccatgat cgccgccctt gatgcgctgc cggtcaacct gatgctggtc
     9361 agcccggtgt atcagatcga ggcgtgcggc gacgagccgc agccggcgac gttgagtgtc
     9421 gccgttccgc caggcgcgca gccggagacc acgctcgatt tgtatgcctg ggattcggaa
     9481 gagcgccaat ggtcgtggat cggcagcagc cacgatctca ccaatggcgc ggtcctggct
     9541 tcgaccgacg cgctgccgga ggctgtggcg ctgatgcaaa cgacctcggt ctcacccgcc
     9601 gtcggcgccg aggtcacgcc gggtgacaag atcagcggcg acgtctcgag aattgtgggc
     9661 gaggtctacc cgacagggct ggtcatcgcc ccggacggct cactcggcgg cgatcgatcg
     9721 agcctgcccg aggcggcaag cccagcccag gcggtatatc cggttgtgcg caactacctc
     9781 gtcggcgggc gccccaaccc cgatctcgtg cgcggcgtcc tcgatgtggc cgaggcgcgc
     9841 cagactcaca tcgacgctct cacggcactg gcagtggagg acgattacgc cggcgtgatg
     9901 ctcgattacc agggcctggt cgagaccgat cgcgaggctt atgccgattt cgtgtccgac
     9961 ctggcatccg ccctgcacgc gcagggcagg caactggcgg tcgtgcttcc agagccactg
    10021 ttcacccctg aagggatggc cgatacgaca ggctatgact gggcgcgcat cggacgggca
    10081 gccgacatgg tgctctcgcc gatggacgac gccgcaatcg tcgcctggac gacgacccag
    10141 gtcgaccgct ataagttcca gcccattctc tcggcgttga gcgttcagtc tgtcgacggc
    10201 tccgaaacgc cgttcacctt cgccgaggca atcgaaatgc tcggcgctgt gaccgccggc
    10261 gaggccgtca gcctgaccgc cgagacgccg gtcaccttca cgctccaagc caagttcccg
    10321 atcgataact tcgcatacaa tccggcggcg ggtatctata cctacgagta tgtcgacaag
    10381 tcgaacgcca cacaccgggt tgtcgtcacc acggcggccg cactggctaa ggcgctggct
    10441 ggagtcttgc cgctccacac gcgcggcgcg gccctgcgcg gcgccacagg cgacacgtcg
    10501 ccgagactga gcgaggtctt acgagcgtac cgccagcagg caacgcccaa gcttgagtcc
    10561 gagatcgaaa tcctctggca gattcagcct gaaaagggtg agtcgtccac ggtcagtaaa
    10621 ccgctgagcg aatcgtggct ggcatggcgc gcgcctgccg agccgggcag gtacaaggta
    10681 tccgcgacca tcgcgggtgt ggacttgggc tctgtggatg tcgccgtgag cgcaggctcg
    10741 ccggaagaag aagtggcggc ggtcaccgcc ggcgttggaa gcggcggtgg tcaggccacc
    10801 tcgcagagcg gcgcggcggc cggcgcggag gccggcggct gctatggcgc cgcctacgtc
    10861 gccgacgtga ccgtgcccga cggcacacga ttcgacaagg gcaaagagtt caccaaaacg
    10921 tggaaggtac gcaacaacgg cacgtgcgcg tggacagccg acaccgagct ggccttcatc
    10981 agcggctcgc aattgagcgc gccgagcacg gtgaaggtcg gcgcgctgga acctggcaga
    11041 gagatcgaaa tcagtgtgtc gatgaagacg tccgacgccg acggcaattt caccggcgta
    11101 tggcaactgc ggaacaagga cggtttctac ggcgactcgt tgacggtggt catcgcggcc
    11161 ggcgagcaga caaccgtcgc cccgcccgtc gcgccggtcg gcaacattgg cagcttcgag
    11221 gccggcgcac acatcgacgg tttccggcgg cccgacctga tgaagtacgc cgggctgaag
    11281 tgggtgaaga ttcagaccta cgccggcgtg gacgtgagcg gcgcgatcga caacgctcac
    11341 gcgcagggct tcaagatctt gctaggcatc gtcggcgaca aagagcgtgt gatggacggc
    11401 gtctatcaag acgaatacgc ggctgccgtc gcggcgatgg ccgccgccgg cgccgacgcg
    11461 atcgaggtat ggaacgagcc aaacatcaac cgcgaatggc ccaccggcca ggtcaacggc
    11521 gcgaactaca cggccttgct ggccaaggct taccgggcga tcaaggccgc caacccgggc
    11581 acgctggtgg tcagcggcgc gctcgcgccg accggcttct ggggggccgc cggctgctcg
    11641 ccggacggct gcaacgacga tacgttcctg gcgcagatgg cggcggccgg agcggccagc
    11701 tacatggact gcatcggtgc gcaccacaac gcgggcgcga ccccgccctc ggcgagcgct
    11761 ggtcacccgg gcggatcgca ttactcgtgg tatttcctac cgacgctcaa cctgtattac
    11821 aactcgttcg gcggtgcgcg caaagtgtgc ttcaccgagc tgggctacct gagcgacgac
    11881 ggctacccgt cgctggagtc gaccgcgccg gctttcgcct gggcgtcgaa cgtgacggtt
    11941 ggccagcagg cggcgtggct ggccgaagcc gccgccatgt ccgcccgcag tggcagggtg
    12001 cggctcatga tcatctggaa cgccgacttc acccgctacg attccgaccc gatggccggt
    12061 tacgccatga ttcgacccga cggatcgtgc ccggcgtgcg acgcgctgcg caacgtcctg
    12121 ggcgggcggt agtcgagcgt atcgacaggc atgggtgggg gcgagcagcc gctcgcccct
    12181 aactgtgtgt tcatatgcag tcttcaatcc aacgtatcct cattcctctt gtcgccatcg
    12241 gtgtaattgc cgttgtgggg attgtgtttc tgctcgcctc gcaggcgaca gtgcctgcct
    12301 gcggcgcgtt cgtcgtgacg gcgggcgctc ctgtgacgct tgacggcggc gcaagcgtcg
    12361 ccctcatcgc cggctcgggc gaattgcgct tcgacagcgc cgcgctgacg gaagccgatt
    12421 tgaccgacga ggtgaatgcg caggcgctgg ctgccgtcaa cgccatgccc gtgaacctgc
    12481 gtgcagaagg tctgatcctc actttcaccc gctgtaacga agagccggcg ccggtgcatc
    12541 tggaaagccc ggcgcccccc tccgctgcgc tacgaccccc gcagacagcg ggcgagggca
    12601 ggtggcaggc gggcgcggcg ctcgacgcgt acgcctggga tggcacgcag tgggcgtggc
    12661 tggccggagc ggccgatcaa ctttccgtcg atctgcccga cctgccgaag atcctcgcct
    12721 gggtagagac gttgccgacc gctccagtca tcggcacgga gcctgatccg cgcacggggg
    12781 gtcttgcgcc agaatactac ggcgtcatca ccgagctgta cgcgccgggt ctgaccgtcg
    12841 ccgcgaacgg ctcggtgaat ggtcaggtgc cggccgcgcc gcctgagggc gcgccgtatg
    12901 tggtgtatcc gctcgtcacc aacgtagcca atgggcaggc cgacgcgacg tcggtcgagg
    12961 caatgctgaa cgacgaagct gcgcgccaac ggcacgtcga ggcgctggcc tctatcgcca
    13021 atggagcgaa cttcgcagga atcgcgatcg attatcaggg ctggacgccc caggcgcgcg
    13081 acgtgttcgc gtcgctcatg gaggacctcg cttcagcgct tcacgcccag ggaaagcaac
    13141 tcttcgtggt gctgccaggc aagtcggcgg ccgggggtcc ttacgacacc gatccggacg
    13201 agtacgattg ggcgcgcatc gggcgggcgg cggacgtggc gcatgtgttg ctgcccttcc
    13261 aggccggcgt ttatcctcct ccacccgaag gcgatgcaga ttcattactg aatcggattc
    13321 ctacgctgat agatcgaaac aagatgcagg ctgtcctctc ggcagccagc gcgcgcacgt
    13381 ccttcggttc gctcacgtcg gctccgtttt cggacacgct cgacgcgttg atcagcagga
    13441 cgccgtcaac gatctcatca accgttggtg tgtcactccc gctcagtctg aacgtgtact
    13501 ttgacccgaa caccgggggc tactcgaatg gcgtcatcga tggccaaatc atcgccttta
    13561 cggcggcgac gctggcctca aagctgaatg ccctttccgg cctcggcctg cgtggcgcgg
    13621 taattcgcga catacaaggt ccggatgtcg cgcccaacct gttcgagccg atcaaggcct
    13681 atcgacagca aagcgcaata tccggctcaa gcgagttgag cgtagactgg actatcaccg
    13741 ccgccgacgg ctcggtcgtg ctgagcgagt cgcggtcgct ggcgagtgcg gacgtggtct
    13801 ggacacccgc cggcgacggc gtgtacacgg cgacggtcac ggccggctct gtgacgaagg
    13861 atgtcgtggt tgtcaccgtc ggtgaagagg aaacagagac cggcgcgggt ggcggcggga
    13921 aagatacccc atcgaccccg gaggccccgt gccttaatgc cgcatacgtc gccgacgtga
    13981 cggttcccga caacacgcgc ttcgacaaag gcaaagactt caccaagacg tggaaggtgc
    14041 gcaacagcgg gacgtgcgcg tggagcgcgg atacggaact cgccttcgtc aggggagcac
    14101 ggctcggcgc gagcagcccg gtgaaggtcg gcgcgctgga gccgggcaag acgatcgacg
    14161 tcagcgtgcc gatggagtcg ggcgacgagg acggcgcgtt caccgggatc tggcggctgc
    14221 gaaatgagga tggcgcattt ggcgatgagc tgagcgtcgt gatcaaggtc ggcgcggagg
    14281 tcgtcgcacc gccgcccatc gcgccgcccg ctggcggcgg gccgactcaa tacggcatcc
    14341 actcccatta ctacggttat atcgatacgg aggccggtgc gcagagtatc gtcaactaca
    14401 cgaacgagct tggcctgggc tggaccaaga ttcagttccg ctggggggat tacgattact
    14461 actgcggcgg gcctgacctg aatcggctta acacgatgat cagcgcggcg aacggcgccg
    14521 gactgaaagt gctgttaagc attgtcacct ccccaccttg cacgcatcca tggacgggcg
    14581 aggtgcacgc gccgccggac gatccggcgg ctctcgccga gctggccggc gggctggccg
    14641 atctgttcaa aggtcgcatt cacgccatcg aagtatggaa cgagcagaac atcagccgcg
    14701 agtggctgac cagcccccag aagctcgacg cggcgcgcta cacgcagttg ctcgccgccg
    14761 cctacaacgc gatcaaggcg aaagacccga acatcctggt catcagcggc gcgctcgcgc
    14821 cgaccggatg gaacgacggc gtgaacgcca ccgacgactt cgaatacctc aggcagatgg
    14881 tcgccgccgg cgcggcgaag tacatggact gcgtcggcac gcacgtcaac gcgctgcgcg
    14941 tgccgccctc ggcgtcgctg ggcggcccgt acgacaacct gttcagcccg ccgcatcact
    15001 cgtggtactt caaggacacg gtgctcggct accagagcat caccggcaag ccggcgtgcg
    15061 tcaccgagtt cggtgtggca tcctcggaga cgatcggtca ggtcgaagga ttcgagtggg
    15121 cgagcgagaa cacgcagcaa gagcaggccg actgggtgac cgaaggcatg agcctgtgca
    15181 agcagtgggg ctgccggctg gtgatcctgt ggaacctgga ctacggcccg gtgacgaggc
    15241 tggtcaccga caatgcgctc tatagcttca tcgacatcgg cctgggccgc aggccggtgt
    15301 tccacgccgt gaagagctgg tgcgcggcga atggatgtag gtgattcatg aatcaagaca
    15361 gacgatcgat ttgggatcgc ataccgacgc cggcgctgat cgtgttgtgg ctgctggcga
    15421 ttgcgttcgg cgcggtgatg gtgttcctcg cgttcttcaa cccggcggcg ccgcaagaaa
    15481 tgccggtgag cacgcccacg gccgtgagcg tcgtgggatc cgtcgccacg gcgacagcgc
    15541 ccgcgacgca gcctgtcgaa ccggctgcgc cggccagcaa tccaacgccg cagccgacga
    15601 ttgcgccgct gcacgatcct tcgatggagt tcggtattca gtcggacgcg ctcaccagcg
    15661 atcccgccta cgcgatcggc ctgctcaggg acaagctgct gctgggctgg gtgaagcagc
    15721 agctccgctg gggcaacttc tcgcccgcgc cgggggaaat ggactggagc gggttcgatc
    15781 gcgtcgtcga agcggcgagc acgaaagaca tccgcgtgct gctcagcatc gtcaccgcgc
    15841 cggagtggtc gcgccccggc gtcgaaggcg tcgaaggccc gccgaacgat ctgaacgagt
    15901 acgccaggtt tgtcggcgaa gtcgtcgatc gctaccaggg caaggtgcag gccttcgaaa
    15961 tctggaacga gcagaacatc gactcggagt ggaggaccaa cccgcaggcg gtcagccccg
    16021 agcagtacgt cgaaatgctg aagctggcgt accaggcgat caaggccagg gatccagacg
    16081 ccatggtcat cagcggtgcg ctgtcgccga ctggcgcctt cggcggcggc tgcgacgcgc
    16141 gtggctgcga cgatcagccc tacctcaagc gcatggtcga gcttggcttc ctgaattacg
    16201 cggactgcgt cggcgtgcat gtcaacggtt acaacttggc gccgcagtac cgccacgacg
    16261 aaggctacaa tgatccgacc gcgtcgttcc gaggcccgtt cgataatccg cacccctcgt
    16321 ggtcgttccg cagcacgctg gaagattatt acaacatcat cggcaagcag aagccgctgt
    16381 gcgtgacgga gttcggctgg gcctcgtacg aaggcatcag cgatacggcg ctgccgtcgt
    16441 acgagttcgg cctggacaat acagccgaag agcaggcaca gtgggacgcg gacgcgttcc
    16501 gcctgatgca agagtggggg ttcgtgaagc tggcgatatt gttcaacctc gacttcgcgc
    16561 agaagtcgcc gctgggcgcg caagatcgca ccgccccgtg gagcatcacc gatctgcagg
    16621 gcgggccgcg gccggccttc gatgccgtgc gtgactttat gaagggagtg ctgggaatcc
    16681 cgtaggaagt cagaatgtcg aatgtggaat gcggaatgaa cgggcgcgat tcattccgca
    16741 ttcactttct ctctcgaaat catcaagccg ggcatggaag cccggcggcg tcggagccgc
    16801 ctcagcttgg cattttcatc agttcgattc cgctgcatac tcaccctcat cgccggtctg
    16861 ctcatgaccg gctgcgcgag cgccacggcc gtgacgccga cgcctgtgcc gacgcctact
    16921 tcttggccgc gcgcccgcct gaagtcgcct gagtacggca tccagatgtt cgcctggtgg
    16981 aagcctgaga tcgccgggcg tgacctgaat ctgatccgcg acatgggttt cacctgggtg
    17041 aagcagtcgt tcgcctggcg cgatattgaa ggtctcggca aaggccaata cgcctggaag
    17101 gtggccgacg acctcgtgcg acgcgccacg aagcgccatc tcaagataat cgcgcgcgtc
    17161 gaccgccagc cattctgggc gcaggcgccg ggtaccgagc cgctggagaa cgcgccgccg
    17221 gccaacctgc aagactttgc cgacttctgc tacgcgctgg ctgatcgcta tcggggccgc
    17281 attcacgcgt accaggtgtg gaatgagccg aacctgtcgc gcgagtgggg aggccagccg
    17341 cccgatccgg ccggatacgt cgagctgctc aaggcgtgct acgtcgcgat caagcgcgcc
    17401 gacccgaacg cgctcgtgat ctcggcgggc atggcgccga ccgggacgtg gaccgagggc
    17461 atcatgcccg atgcgatgtt cgtcgagaag atgtacgaag ccggggcagc gccatacttc
    17521 gacctgctgg gtgcgcacgc gccggggttc aaagcgccgc cggaagcctc gcccgacgag
    17581 gtggcggcga accctgagct gggcgggcag cgcttcttct ccttccggca cgtcgaggac
    17641 ctgcgcgaga tcatggtcag gcacggcgac gccggcaagc aggtggcggt gctcgagatg
    17701 ggctggacga ccgacccgat ccatcccgaa tacgcgtggc acgccgtcga cgagcagacg
    17761 caggccgatt acctggtgcg cgcgttcgac ttcgccaggc agaactggtc gccgtggatc
    17821 agcatcatga ccgtgatcgg gctggccgat ccggtatgga caccagacga cgagcaatac
    17881 tggtggacgg tggtctatcc cgattggccg gagacgcgcg tgaagccggc gtacgaggcg
    17941 ctgaaggcaa tggagaagaa atgacgaacg cagaatgagg aatgctgaaa ttctacattc
    18001 tacattctgc attccacatt ctgctataat tcttttatgc tcacgctgaa tgcccccggg
    18061 ttgctggcac tcagtttttt cctgcatttg attgcgacgg tcgtgtggat cggcgggctg
    18121 gtgacgatgg cgctggtcgt gtggcctggc ctgcggcgcg cgctcggcga cgacgaccga
    18181 aggctcgcgc gcgccgtcga agcgctcgac cggcgcttca agccgctggc gaacctcagc
    18241 ctggtcgtac tgatcctcac cggcctcgtg caaatgaacg ccaatgagaa ctacaagggc
    18301 tttctgaact tcgacaacct gtgggcgcag gccatcctgc tcaagcacgc cgcgatcatc
    18361 ggcatgatcg ccgtgggcgc gctgctgcaa ttcggggtgc agcctgcgct gcagcgcaac
    18421 gcgatgctgg cagcggcggg cgtctcgaac gaggaagaag tggccgcact gcggcagcgc
    18481 atggcgcgcc tgggccgcat caacctgatc ctgggcgtgg tcgtgctgat actcacagcg
    18541 atcgctcgtg tccagtgaat gacgacagac gaaaggcgtg tcgaccagcc tcgcgggacg
    18601 atatgcgata tgcgataagc gatacgcgat aagccatcat gttcgaaatc gtctttctcg
    18661 gcacctcagc cgccgcgccg tcgatccgcc gtaacctgtc ggcgcacgtc gtgctgcaca
    18721 aagagtatcg ctttctggtg gactgcggcg agggcacgca gcgccaactg ttgaagagcg
    18781 ggcttggctt caaaaggctg aataggatcc tgctcacgca cagccacctc gaccacatcc
    18841 tgggcctcgg cgggctgctg agcacgttca tgcgctggga gacgatcgag gaggtagaga
    18901 ttttcggcgg gcgcagcacg ctggatcggg tggaggactt gatttaccgg gtcgtgctgc
    18961 gcggcgcgcg cccgccgatg cgcctgtcgt tcgtcgacgt caagccgggc aggctgctgg
    19021 aggacgactc gttcgagctg atcgcgttcc cggtgatgca ccggggcggc gagtgcttcg
    19081 gctatctgtt tcgggagaag ccgcgccggc cattcctcgt cgacaaggcg gaggcgctcg
    19141 gcgtgccgcc cggaccggaa cggcggcggc tggtcgcggg cgaatcggtg acgctgagcg
    19201 acggccggac gatccacccc gacgaagtcc tcggcccagt gatccggggt gcggcgctgg
    19261 cgcacgtggg ggacgccggg cgcacgtcgg atctgatcga gcacgtgcgc ggcgcggacg
    19321 cgctcgtcat cgagtcgacg tacctggacg ccgaggccga tctggcgcgc tcgtacgatc
    19381 acttgacggc ggcacaggcg gcgcgcctcg cgcaggacgc gcaggtcggg cagttgattt
    19441 tgacacacat ctcgcgccgg tacggccaaa agcaggtgct cgccgaggcg caggcgatct
    19501 tcccgaatac ggccgtcgcc cgcgatttcg atcgtttccg cgtgctgaaa gacaagtcca
    19561 tgctgttgga gaacctgatc gtggaagagg agaactggca tgtcgctgcg taaggctgcc
    19621 gtcctggacc tggcaagcgc cgcgtatgag gtggacgccg aggtgaagcg gggcattttg
    19681 aagaaggccg atgggcactg gacggtagcg gatcaggaga tgagtgagtg gctagaccgc
    19741 ttcgaggggc acgaggtggt cgtgatcgtc gcgtcgatga gcgacgagaa gccgatcgag
    19801 ccgcgcatct gcacgcgctg cggatacgag ttcgtcggcg tggagtgccc gcgctgccgc
    19861 gcggcgcgca tccgcctgcg cggcgagtga tggcgtgagt cgggggccga gtggtttcat
    19921 tagggggtaa gcgttcagag gaacgactat tctaaccacc aagacactaa gacacgaggt
    19981 ttgagagtga gtaacgaccg ttgaagtcgt tgttccggca gagcactgcg ccggcagcga
    20041 ggcaggtagg gatagtttct tggtgtcttc gtgcctttgt ggtgaagatc gcgtcacgat
    20101 ctgagcggtt accattaggg gaatgtcgct ttcaaacggt aaggcgcgtc aggatcgccg
    20161 ttttctgagt agatgcaagc gtaatatctt cctgcagcgc caaagtgctc aatgtgatag
    20221 ggtggtgtag tatcctgtgt tggatctggg ttctctatct ggaagaataa agctaaatgt
    20281 atctgtcgac cggtatggtt ctccaagtcc acagttatta gtccagcact cgtcgcaaag
    20341 aaccggaagc aatcccactt gtcatccacc gggccgggct tgccatagta ccacaggcca
    20401 gatagcagag gcccgttgga ctcgtcgggc gcgttgggcc cgtcaatata attgtttggc
    20461 tcaagctccc atagcacgtc tacgctctcc tcgttggacg ggcggttgct gcccgaactg
    20521 ctgattgatt tgacgcggta ataatacctt gttggaccac gatcactcaa aggagcgtag
    20581 gtgtcactat ctgagtagac caccataggg ctgatagagc caaagacagt ttcttccaaa
    20641 acatagtatt ctgcgccaga agcacaccac cgcaccacgt agcttccgtc gttatcatcg
    20701 ttctcgattg gaaataacgt taaaccgaaa caggcatcgc gcagcaccga aggcaaatac
    20761 accttcttgg gagagaaatc gatggcgaca acgtgagtgc ctgtaacggc attgttggca
    20821 gcattcgtag ccactacgga gaagatcttg atgcccgtgg tcgtccaggt gtaagtgacg
    20881 acaggcccct gacccgaatt cggcggtgga gaccaagtat aggtgatggg cttgtaagca
    20941 ttaggtggac tggcgatggc tgtgaaattg tatgcggtgt tgatcgtgcc tgcctgcgtg
    21001 ccattgatcg cgatatcatc aagcggctgg ggctgaatat tgacgatgtg aacgtcgtcg
    21061 acagtcttat caccgttgcc ggcgacgacg gtaatgatct tgtttccagg taccgcccaa
    21121 gtgaacgttt gtgtatcgta cagagaatcg gtgacgattc gtgtaaaact ccctaaatct
    21181 gagacctgcc aggtaaacgt gaccggtcgc gtggtgcttt tcggccggac agtggcagta
    21241 aattcataac ctacttcagt atagcctgtc gaccgtccgg taatatctac tgagttaggc
    21301 ggggcactgg ggagttcggc tgaaagagaa ctaccatgta actgttcagg cgtcattgcg
    21361 aggagcgtct tttgcgacga agccatcgcc tggccggctg ggggattgct tcgcacaaag
    21421 atgctcgcaa tgacgttggg ccgcgtagtt acgctcactg ttattttgtc acgcgaagcc
    21481 aaatccaatg cctgttgtga cgcacggaca atcagggcaa acgtaacgcg gacaaacgag
    21541 agtgcgatga tcagagcggc agatgagaac gctaccttcc tcattgccgt attagcatat
    21601 cacagtagcg ttggaatccg gttgggccat agccccacag tacttatggg attgccgtga
    21661 acaccacagt tagagcgtgt ttggagattc aagtttatag tggcttccgt tttggtttac
    21721 gccggagtcg agcctttagg cggttgccgg ctaaagcctc gactccgagc gcgacgtaaa
    21781 cgcaatcgga aagcgcccta agcggcagtt gcactgcggc gaaaatgcta tttcaaaaca
    21841 ggctcttagg gtgccgagga cgacgtggtg gacgtacgcg taggaaacgg tggaagtgta
    21901 gccgtaggat taggcgtacg cgtagcgaac ggtggaactt ccggtgtggg tgaatctggc
    21961 acgggcgctg gtgtgaatgt ccgtgtcggt gacgaagcgc gtgttggggt gcgacgcaat
    22021 gcgggcgtga ccgttgccgt acgtgtgacg gttagcgaag tcgttcgtgt cggcgatgga
    22081 gtacgcgttg gggtcggcgt aagggtaggg gttatcgtca atgtgagcgt atgcgctcct
    22141 gctgacaggg tggaggtggg agtgcctgct ttaggcacaa ttccagacac ccacagtgga
    22201 ccaagaatgg cagctaaaac cagaagaata atgagcaggg cgccggagaa agctagcttt
    22261 gcacggtttc ggcgcagcca cgctcgtgct ttcgcttcga gcgtctcccc atgcggcggt
    22321 ccctcaaggt ctatggctgc ctgagcctgg cggctaagtg cccgtctgat cgattccagg
    22381 tcatccagca cctcgcgagc cgcctgatag cggcggcccg gatcggtgtg cagggctttc
    22441 tgcaacgcgg cgacaaactg ctgcggcacg ccattctcga taagaaacgt ttcgggaaat
    22501 ctatccagca tcgctgcatg gtcagcagag ccgtcggcaa gcaattcttt cagaaccttc
    22561 cccgt
//
LOCUS       DBNHXB010000340        11261 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3446172, whole genome shotgun sequence.
ACCESSION   DBNHXB010000340 DBNHXB010000000
VERSION     DBNHXB010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11261)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11261)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11261
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3446172"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="AC162G_13805"
     CDS             complement(<1..112)
                     /locus_tag="AC162G_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR5076926.1"
                     /translation="MPNRAQDWFNQAVRDLDQAEDSRRAGRHEWACFAAQQ"
     gene            326..1105
                     /locus_tag="AC162G_13810"
     CDS             326..1105
                     /locus_tag="AC162G_13810"
                     /inference="COORDINATES: protein motif:HMM:NF023818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HIR5076927.1"
                     /translation="MSKLARIESLIEQLVEGTFGRLFSGTLQPIEVAHALARALEDYQ
                     VTDDQGRRFAPNVFWVYLHPSDYEALRETQPTLPEDLAGSVDELARRADLLMPDWPVV
                     EIVASEETPQGRVSVAARYIAQETSPIGPTQEIAPDEIRRALDASRLRAYLIMDGKRH
                     VPLTRSITTLGRALDNDLVIDDGRVSRHHAQIRLRSNRYVVYDTGSSGGTTVNSQPVT
                     EHSLEAGDVISLAGYTLVYYEDAPLPAEPASTLEDTPMLHE"
     gene            1200..1643
                     /locus_tag="AC162G_13815"
     CDS             1200..1643
                     /locus_tag="AC162G_13815"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR5076928.1"
                     /translation="MEVALLLARILLIILLYAFLGLIVILLWRDVRSATRREAASTNR
                     PRSARLVLLDHVDGMRDGDAFALKPYTSIGRAPSNTIELPDSYCSAEHALIVWRNNQW
                     WLEDRDSRNGTLLNDVSIDTPVVIGAGDVIRMGRVRMRLEVEANG"
     gene            1683..2471
                     /locus_tag="AC162G_13820"
     CDS             1683..2471
                     /locus_tag="AC162G_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011317197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5076929.1"
                     /translation="MDLGLKDKVALVTAASKGLGKAAALRFAREGARVALCARSELVD
                     RAAEDIRRETSTDVLPVRADVTQPADVERLIDATVDRFGGLDILVTNAGGPPSGTFDN
                     FDLETWQQAVDLTLLSAVRLIKAALPYLRQSTAPAILTMNSISTKQPIANLTLSNSIR
                     MAVIGLTKTLSQELGPDGIRVNSILPGWTYTERVDELMKARATKSGRTPEEEAALITA
                     NNPLKRMGRPEEFANVAVFLCSPAASYVNGVMLQVDGGSYAGTM"
     gene            2608..2994
                     /locus_tag="AC162G_13825"
     CDS             2608..2994
                     /locus_tag="AC162G_13825"
                     /inference="COORDINATES: protein motif:HMM:NF014021.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR5076930.1"
                     /translation="MKQVSSAEELTQLRPAERVALSGYLARLRARFANRVLHVILFGS
                     RARGEGDAESDLDVLVVVDDGDWRFHDAIADESVEPWLAHGALISPVTMNRREYEQLR
                     EWRTLFCRNLEQDGVDLWIAEPVSIS"
     gene            3178..4494
                     /gene="miaB"
                     /locus_tag="AC162G_13830"
     CDS             3178..4494
                     /gene="miaB"
                     /locus_tag="AC162G_13830"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR5076931.1"
                     /translation="MKYHLHVFGCQMNEADIQRVGSELEKLGLSSASDAASADVIVLN
                     TCVVRQSAEDKAHSYIQTLKPIKQRHPDKVITVMGCMVGVKGGAALRQAYPWVDVFMP
                     PSETRPLVDYLRQRDAYQLLERETRERYAMQDGDLILPAAERGRLVSAHVPVVYGCSH
                     ACTFCIIPFRRGPERSRPVGEIAREVRSLAAQGVKEVTLLGQIVDRYGKDAPGGPDLA
                     DLLRVVHEIDGIQRIRFLTSHPNWMNDRLLNAVAGLPKVCEQIEVPVQAGDDEVLQNM
                     RRGYTRDDYRRLIDKIRRKIPGASIATDIIVGFPSETEAQFQKTYDLLAELELDVAHL
                     ARYSPRPQTVAQRSMSDDVPEEEKMRRFRALEDLQTHIADKINRRYLGETVEILVEAS
                     HKGKWRGRTRTNKLVFFEDPVDRLGQAVNVKITWSGPWSMQGALAA"
     gene            complement(4542..5210)
                     /locus_tag="AC162G_13835"
     CDS             complement(4542..5210)
                     /locus_tag="AC162G_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5076932.1"
                     /translation="MKKHLRIIIVDDHEVVRLGLKALLERHPDFTVVDEASSAREAVQ
                     KAIAHRPEVVVMDIRLPGGSGIDACREIVQALGDETKVIMLTSYAEDEMLFEAIAAGA
                     AGYVLKQIGADDLVHAIETVGRGEALLDPALTQRVFERVREAQRKEEKSAFSDLTDQE
                     MRVLALLAEGKTNREIAQLLYLGEGTVRNYVSSVLSKLGLANRAEAAAYAVQHKIKSF
                     QTDG"
     gene            complement(5288..6859)
                     /locus_tag="AC162G_13840"
     CDS             complement(5288..6859)
                     /locus_tag="AC162G_13840"
                     /inference="COORDINATES: protein motif:HMM:NF024640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076933.1"
                     /translation="MEVSTPGFDAYQRTAIGAAPESDWRSQLCALLSVVGDGCIEPTA
                     YDTAWVARVPHCEFPDRPAFPQALEWLVAHQHADGSWGTRLNYYHDRVISTLSAALTL
                     ARWNEHVDSGAWEDRIEAAQRAIQRDAAGMPSDPYDTIGFELILPTLLDEAGRFGLDV
                     PYDVFATDQGVARMREAKLKRIPPELIYTRRISTVFSAEFLGDELDVARVKDLQDEIG
                     SIASSPSATAYVLLKDPDNLAARDYLAGVLARSGGAAPPVDPIDVFEPAWTLYNASLV
                     WPDASQVAAAIEPHIAALAREVQRKNGAGYNSYFVVPDLDGTAMVFRALTWAGTAPEP
                     ALLAQFEEDDHFRCFPYERNPSISAHVHLLDALRAAPPFPDRERMVRKAVAFLNRSRT
                     LRTFWFDKWHASPYYTTAHAITALREDADLAADAVYWIVNTQNEDGSWGYYATSTAEE
                     TAYCVQALVAYGRDQAASPGLRDAIGRGVDYLVSSAERIDCRYTPLWLAKSLYTPKWV
                     VHSTVLSALALAESF"
     gene            complement(7037..8341)
                     /locus_tag="AC162G_13845"
     CDS             complement(7037..8341)
                     /locus_tag="AC162G_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HIR5076934.1"
                     /translation="MTAWFDMYRDKIVTPEQAAAAIQSGQRIFLSGNCSVPQQVLAAL
                     VARASELRDVEIVQVLTVGNADYVAPEMTGHLRVNTLFISDNVRRAVNEGRADFTPCF
                     LSEIPGLFKNRYLPLDAALIHVSPPDEHGFCSFGVEVGVTKTAAQAARAVIAEVNPHM
                     PRTLGDSFIHVSKLDTIVPVDYELPETPMGMTTELARRIAAHIAGLIPDGATLQTGIG
                     AIPDAVLHQLTHHTHLGVHTELFSDGVIDLVMQGVIDGEMKSLHPGKIVAGFMLGTRR
                     LYEFVDDNPIVELHPSEYVNDPFVIAQNDRMVAINSAIEIDLTGQVCSDSIGARLYSG
                     VGGQIDFIYGASRSQGGVPIIAVPATATIQGDVVSRIVPTLKPGAGVVTTRNHVHTVV
                     TEHGVANLYARTIRQRARALIDVAAPEFRAELEQKAIELRYL"
     gene            complement(8338..9444)
                     /gene="ald"
                     /locus_tag="AC162G_13850"
     CDS             complement(8338..9444)
                     /gene="ald"
                     /locus_tag="AC162G_13850"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR5076935.1"
                     /translation="MHIGIPCERRPNEYRVGLTPAGVELLTAGGHHCYVERSAGRGAG
                     FSDVDYERAGARIVYSPEEAYGRADLVLKVSRPIAEEVGWLRDGCILMGFLHLAAGRR
                     ERVEGLLDKRVTAIAYETIQNDDGSLPVLKPLSQAAGRMAPQVAATLLQNDRGGKGIL
                     LGGVPGVPPAEVVIIGAGTFGTAAARAFLGAGASVYVLDRDLWRLQQLDEMHQGGGLV
                     TMVSHSFNIRKVARFADVLVGAVLEPGARAPIVVTREMVQSMRPRSVIMDVSIDQGGC
                     VETSRPTTHRDPTYVEEGVIHYCVPNMTAVVARTATHALNIAAWPLIWEIAHKGLDAA
                     LGSLPALRRGVATRDGRVVNAALAAALGLREAAE"
     gene            complement(9455..9958)
                     /locus_tag="AC162G_13855"
     CDS             complement(9455..9958)
                     /locus_tag="AC162G_13855"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5076936.1"
                     /translation="MYRNIIVPLDGSETAEQVLPYVRDLVRGRTVTVHLISVAPLSAT
                     APVSAVAPLRMYPLVVSRADFEAQTQERNRIESELRNYLNGVAMTLAQGNVATSVEVR
                     FGEPAEEILALQSEVRADLIAMCTHGRTGLARWAYGSVAEKVTRHADCPVLLVRARKT
                     NSSASRS"
     gene            complement(10111..11190)
                     /locus_tag="AC162G_13860"
     CDS             complement(10111..11190)
                     /locus_tag="AC162G_13860"
                     /inference="COORDINATES: protein motif:HMM:NF033591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HIR5076937.1"
                     /translation="MDRTTLYRTWRTRLAEYIPDECSTRQTNLLWLIVGLYLARSVYA
                     SAIVRQWPVVAKVVSLTRRLSRFLDNPAVEVRTWYRATAQQVLGLWAGDKVTLIMDAS
                     QVGFGHQLLMVAAAYRRRAIPIAWCWVRGQRGHSSAATQLALWAYVRRLVPTDTQVIV
                     VGDAEFGSISVLEQLARWGWSYVLRQKGSHRVAAHRRRTWHALADLVTHPRQWAWWPQ
                     ARLTQQWAYHTTVFAYWAKGENEPWLLATNLPDRYAARRAYARRMWIEEMFGDLKDHG
                     FDLEATHLRHFLRLNRLTLAVCLLYVWLLFIGHAVIRAGQRHWVDRRDRRDLSLFRIG
                     ADFIERCLILLWPFPKVQLPQVSGG"
BASE COUNT         2117 a         3687 c         3521 g         1936 t
ORIGIN      
        1 cctgctgcgc tgcaaagcag gcccactcgt ggcggcctgc ccgccgggag tcttcagcct
       61 gatccagatc cctcacggcc tggttaaacc aatcttgagc gcggttaggc acgttgcttt
      121 acccccaagt atagcgcgca taccgatagg tcactcagcg caccatggcg tcaggcggtg
      181 tgacagccta cctaaagtat acctggttta ataccatgcg ccaagacgtt tgccgaacac
      241 cagccgtggc attataatcc gcacgagcca cccaaagact tcggaagtcc gcgacagact
      301 tccgaagtct caaacggaag gaatcttgag caaactcgca cgtatcgaat cgctaatcga
      361 gcagttggtc gaaggcacgt tcggccgcct gttctccggc acgctgcagc caatcgaggt
      421 cgcccacgcg ctggcgcgcg cgctcgaaga ctaccaggtg accgacgacc agggccggcg
      481 cttcgcgcca aacgtcttct gggtctacct gcatccgtcg gactacgagg ctctacggga
      541 gacccagccg acgctgccgg aggacctggc aggctcggtc gacgagctgg cgcggcgcgc
      601 cgatctgttg atgcccgact ggccggtcgt cgagatcgtc gcctccgagg agacgccgca
      661 gggacgagta tccgtcgccg cgcgatacat cgcccaggag acatcgccta tcggcccgac
      721 ccaggaaatc gcgccggacg agattcgccg ggcgctggac gccagccgcc tgcgagcgta
      781 cctgatcatg gacggcaaac gccacgtccc gttgacgcga tccattacga cgctcggccg
      841 ggctttggac aacgacctgg tgatcgacga tgggcgcgtg tcgcggcacc acgcgcagat
      901 ccggctgcgc agcaaccgct acgtcgtcta cgacacggga tcgagcggcg gcacgacggt
      961 caacagccag ccggtcaccg agcacagcct ggaggcaggg gacgtgatca gcctggccgg
     1021 atacacgctg gtgtattacg aggatgcgcc actgccggcc gagcctgcca gcacgctcga
     1081 agacacgccc atgctgcacg agtaactcac gttacgcagt gccgcatcct tcgaccgcac
     1141 ggcgcgcact gctccgctca ggatgcgctg cgtaaggtga gtgagtaaat gttgatcgta
     1201 tggaagtcgc cctgctgctc gcccgtatcc tcctgatcat tctgctctac gcctttctgg
     1261 gactcatcgt catcctgctg tggcgcgatg tgcgcagtgc gacccggcgt gaggccgcgt
     1321 cgaccaaccg cccacgctcg gcgcgcctgg tcctgctcga tcacgtcgac ggcatgcgcg
     1381 acggagacgc cttcgccttg aagccgtata cttcgatcgg ccgcgccccg agcaacacga
     1441 tcgaactgcc cgactcgtac tgctcggccg agcacgcgct catcgtgtgg cgcaataatc
     1501 agtggtggct cgaagaccgc gacagccgca acgggacgtt gctcaacgac gtctcgatcg
     1561 acaccccggt tgtgatcggc gccggcgacg tgatccggat gggccgcgtg cggatgcgcc
     1621 tcgaggtgga agcaaatggc tgatagctga tagctgatgg cagatagcaa aaaggagaaa
     1681 gcatggatct cggactcaaa gacaaggtcg ctctcgtcac cgccgcttca aaggggctgg
     1741 gaaaagccgc ggcgctgcgg ttcgcccgcg agggggccag ggtagcgctt tgcgcccgca
     1801 gtgagcttgt cgacagggcc gcggaagaca tccgccgcga aacgagcacc gacgtgctac
     1861 ccgtgcgcgc cgacgtcaca cagccggcgg acgtcgagcg cctgatcgac gcgactgtcg
     1921 acagattcgg cgggctggac atcctggtga cgaacgccgg cggcccaccc tcaggcacct
     1981 tcgacaactt cgatctggaa acctggcagc aagcggtcga cctgacgctg ctcagcgcgg
     2041 tgcgcctgat caaggccgcc ctgccctacc tgcgccagtc gaccgcgccg gcaatcctca
     2101 cgatgaactc gatatcgacg aagcagccta tcgccaattt gacgctgtcc aactcgatcc
     2161 gcatggccgt catcggcctg accaagacgc tgagccagga actcggccca gatggcattc
     2221 gcgtcaacag catcctgccg ggctggacgt ataccgaacg ggtcgacgag ctgatgaaag
     2281 cgcgcgcgac gaaaagcggc aggacacccg aagaagaagc cgcgctcatc accgccaaca
     2341 atcctctgaa gcgcatgggg cggccggaag agttcgccaa cgtcgccgtc ttcctgtgct
     2401 cgccggcggc gtcgtacgtc aacggcgtga tgctgcaggt ggacggcgga agttacgcag
     2461 ggacgatgta gtcagatggt caagtggtcg gatagccgga tagtcagata atcagttaga
     2521 tcggcagttg cgattcgtac ccgaactatc ctcccattcc tgccacgaac gcctcatagc
     2581 ccatcaattg ctgtataata tcgctcgatg aagcaagtaa gctcagcgga agaactgacg
     2641 cagcttcgcc cggcagagcg cgtggcgctc tcgggctatt tagcccgctt gcgggcgcgc
     2701 tttgccaatc gcgtgctgca tgtgatttta ttcggctcgc gagcgcgcgg cgagggcgat
     2761 gccgaatccg atctggacgt actggtcgtc gtcgacgatg gcgactggcg ttttcacgac
     2821 gcgattgccg atgagtcggt ggaaccgtgg ctggctcatg gtgccttgat ttcacctgtc
     2881 acgatgaatc gaagagagta tgagcagttg cgggaatggc gaacgctatt ctgccgcaac
     2941 ctagaacaag acggggtcga tctatggatc gccgaacctg tgtcaatctc ataaagcttt
     3001 ggctggagaa ggcccgcgac gacctggcga cggcagagga tgattaccag cccacagccc
     3061 cggtgcccac actggaacaa gccagcacac gtctggatga gagccgccgg tttgttgacc
     3121 gtattgaacg ctatctccgc gaggtcgggg cgctagaata aggcagcgat tgtcatcatg
     3181 aagtatcatt tgcacgtctt cggctgccag atgaacgagg ccgacatcca gcgggtcggg
     3241 tcggagctgg agaagctcgg cctgagcagc gccagcgacg ccgccagcgc ggacgtgatc
     3301 gtcctcaaca cgtgcgtcgt gcgccagtcc gccgaagaca aggcccatag ttacatccag
     3361 acgctcaagc cgatcaagca gcgccatccc gacaaggtca tcacggtgat gggctgcatg
     3421 gtcggcgtga agggcggcgc cgcgctccgc caggcctacc cctgggtgga cgtgttcatg
     3481 ccgccctcgg aaacgcgccc tctcgtcgat tacctccgcc agcgcgatgc gtaccaatta
     3541 ctggagcgcg agactcgcga gcggtacgcg atgcaggacg gggacctgat actgccggct
     3601 gcggagcgcg gccggctggt cagcgcgcac gtgcccgtcg tctacggctg ctcgcacgcg
     3661 tgcacgttct gcatcatccc cttccggcgc ggcccggagc gcagccgccc cgtcggtgag
     3721 atcgcacgcg aagtgcgctc gcttgccgcc caaggcgtca aggaagtgac gctgttgggg
     3781 cagatcgtcg atcgctacgg caaggacgcg ccgggaggcc ccgacctggc cgacctgctc
     3841 cgcgtcgtcc acgaaatcga cggcattcag cgcatccgct tcctgacctc gcatccgaac
     3901 tggatgaacg accggctgct caacgccgtc gccgggctgc ccaaggtgtg cgagcaaatc
     3961 gaagtgccgg tccaggcggg cgacgacgag gtgctgcaaa acatgcggcg cggctacacg
     4021 cgcgacgact accggcgcct gatcgacaag atccgccgca agattcccgg cgcgtcgatc
     4081 gcgacggata tcatcgtcgg cttccccagc gagacggagg ctcagttcca aaagacgtat
     4141 gacctgctgg ccgagctgga gcttgacgtc gcgcacctgg cgcgctactc gccgcgcccg
     4201 cagacggttg cgcagagatc gatgagcgac gacgttcccg aagaagagaa gatgcgaagg
     4261 ttccgggcgc tggaggatct tcagacccac atcgcggaca agatcaatcg ccgctacctg
     4321 ggcgagaccg tcgaaatcct cgtcgaagcg agccacaagg gaaagtggcg aggccggacg
     4381 cgcacgaata agctggtctt ctttgaggac cccgtggatc ggttgggcca ggccgtgaac
     4441 gtgaaaatca cgtggagcgg cccgtggagc atgcagggcg cactcgcagc gtgaaacaga
     4501 acagaacccc ggtttcttcg agaaaccggg gttcttcttt cttaaccgtc tgtttggaac
     4561 gacttgatct tgtgctgaac ggcgtaggcc gccgcttcgg cgcggttggc taaacccagc
     4621 ttgctcagga cgctgctcac gtagttgcgc accgtgcctt cgccgagata gagaagctgc
     4681 gcgatctccc ggttcgtctt gccctcggcc agcaaggcca gcacgcgcat ttcctggtcg
     4741 gtcagatcgc tgaacgccga cttctcctct ttgcgctgcg cctcgcgcac gcgctcgaag
     4801 acgcgttggg tgagcgccgg atcgagcagc gcctcgccgc gcccgaccgt ctcgatggcg
     4861 tgcaccaggt cgtcggcgcc gatctgcttc aggacgtagc ccgccgcgcc ggccgcgatc
     4921 gcctcgaaga gcatctcgtc ctcggcgtag gatgtcagca tgatgacctt cgtctcgtcg
     4981 cccagcgcct ggacgatctc gcggcaggcg tcgatgccgc tgccgcccgg cagccggata
     5041 tccatcacga cgacctcggg acggtgcgcg atcgctttct gaacggcttc cctggccgag
     5101 ctggcttcat cgacgaccgt aaaatcggga tggcgctcga gcagcgcttt caggccgagc
     5161 cgaacgacct cgtgatcgtc gacgatgatg atgcggagat gctttttcaa ccccgtcccc
     5221 ttcctgccct gtccgacttt aatatacccc aacccaggca ggcaggcaaa cgaagacggg
     5281 tgattattta gaaggattca gccaaagcca gcgcgctgag cacggtcgaa tgcacgaccc
     5341 acttcggggt atacaagctc ttcgcgagcc agagcggcgt gtagcggcag tcaatgcgct
     5401 ctgccgagga aaccaggtaa tcgacgccgc gcccaatcgc atctcgcagc cccgggctgg
     5461 ctgcctgatc tcgcccatac gccacgagcg cctgaacgca gtaggctgtt tcctcggccg
     5521 tgctcgtcgc gtagtagccc cacgatccgt cctcgttttg cgtgttgacg atccagtaga
     5581 cggcgtccgc tgccaggtcg gcatcctcgc gcagcgcagt aatcgcgtgc gccgtcgtgt
     5641 aatacggcga ggcatgccat ttgtcgaacc agaacgtgcg cagcgtgcgc gagcgattca
     5701 gaaacgccac ggccttgcga accatgcgct cccgatcggg gaacggcggc gcggcgcgca
     5761 gcgcgtcgag caggtgcacg tgcgcgctaa tcgacgggtt gcgctcgtag ggaaagcagc
     5821 gaaagtgatc gtcctcctcg aactgcgcca gcaaagccgg ctcgggcgca gtccccgccc
     5881 aggtcagcgc gcgaaagacc atcgccgtgc cgtcgaggtc cggcacgacg aaatacgagt
     5941 tgtatcccgc gccgttcttg cgctgcactt ccctggccag cgcggcgata tgcggctcga
     6001 tcgccgcggc aacctggctc gcgtcgggcc ataccagaga tgcgttatac agcgtccacg
     6061 ccggttcaaa tacgtcgatg ggatcgacag gcggcgccgc gccgccgctg cgcgccagaa
     6121 ccccggccag gtagtcgcgc gcggcgagat tgtcagggtc tttcaagagg acataggccg
     6181 tcgccgaggg ggaggacgcg atcgagccga tttcgtcttg caggtccttc acccgcgcga
     6241 cgtccagctc gtcgccgagg aattccgcgc tgaagaccgt ggagatgcga cgcgtgtaaa
     6301 tcagctcagg cggtatgcgc ttcagtttgg cctcgcgcat gcgcgcgacg ccttggtcag
     6361 tggcgaacac gtcgtagggc acgtcgagac cgaagcgacc cgcctcgtcc agcagcgtcg
     6421 gcaagatcag ctcgaaaccg atcgtgtcgt aagggtcgga cggcatgccg gccgcatcgc
     6481 gctgaatggc gcgctgagcc gcttcgatgc gatcttccca cgcgccgcta tccacgtgct
     6541 cgttccagcg agccagagtg agcgcggcgc tgagcgtgga gatgacgcgg tcgtggtaat
     6601 agttcaaccg cgtcccccaa ctgccgtcgg catgctggtg cgccacaagc cattccagcg
     6661 cttgcgggaa agccggccgg tcggggaatt cgcaatgcgg gacgcgcgcc acccacgccg
     6721 tgtcatatgc cgtcggctcg atgcagccat ccccgacgac agatagcaga gcgcagagtt
     6781 gagagcgcca atcgctctcc ggcgccgcac cgatggccgt gcgctgatat gcatcgaaac
     6841 caggagtcga gacttccata ccctctcgtt ccaggcgcct gaacagcaca aggcgcccgc
     6901 tcacccgccg aagtccgtcg agcgcagcgt ggggctagcg tctgagagcg tatcatatcc
     6961 tggttaggaa aaggtatgcg cgaggtcagt aaatcctttc aggagttact ttgagcgtta
     7021 cagtagaatt gcagaactag aggtacctca gttcgatggc cttctgctcc agctcggcgc
     7081 ggaattccgg ggctgccacg tcgatgagcg cccgcgcccg ctgccggatg gtcctggcgt
     7141 acaggttggc cacgccgtgc tcggtgacca cggtgtggac gtgattgcgc gtcgtcacca
     7201 cgcccgcgcc cggcttcaag gtcggcacga tccggctgac aacgtcgccc tggatcgtcg
     7261 ccgtcgccgg cacggcgatg atgggaacgc ctccctggct gcgcgacgcg ccgtagatga
     7321 agtcgatctg gccgcccacg ccgctgtaca ggcgcgcgcc gatgctatca gagcacacct
     7381 gcccggtcag gtcgatctcg atcgccgagt taatggcgac catgcgatcg ttctgggcga
     7441 tgacgaacgg atcgttcacg tattcggaag ggtgcagctc gacgattgga ttgtcgtcga
     7501 cgaactcgta caggcgccgc gtgccgagca tgaagccggc gacgatcttg ccggggtgca
     7561 gcgacttcat ctcgccgtcg atcacgccct gcatcacgag atcgatgacc ccgtcggaaa
     7621 acagctcggt gtgcacgccc agatgcgtat gatgggtcaa ttggtgcagg acggcgtcgg
     7681 ggatcgcgcc gatgccggtc tgcagtgtcg caccgtcggg gatcaggccg gcaatgtgcg
     7741 cggcgatccg gcgcgccagc tcggtcgtca tgcccatcgg cgtctcgggc agctcgtaat
     7801 cgacgggcac gatggtgtcg agcttcgaca cgtggatgaa cgagtctccc agcgtgcgcg
     7861 gcatgtgcgg gttgacttcg gcgatgacgg cgcgcgccgc ctgagccgcc gtcttcgtca
     7921 ccccgacttc gacgccgaac gagcagaagc cgtgctcgtc gggcgggctg acgtggatca
     7981 gcgccgcatc cagcggcagg tagcgattct tgaacaggcc ggggatctcc gacaagaagc
     8041 acggcgtgaa atccgcccga ccttcgttga ccgcgcgccg cacgttgtcg ctgatgaaca
     8101 gcgtattgac acgcaggtgg ccggtcattt ccggcgcgac gtaatccgca ttgccgacgg
     8161 tcagcacctg cacgatttcg acgtcgcgca gctccgaagc gcgcgccacc agcgcggcca
     8221 gcacttgctg gggcactgag cagttaccgc tgaggaagat acgttggccg gactggatgg
     8281 ccgcagcggc ctgctccggc gtgacgatct tgtcgcggta catatcgaac cacgccgtca
     8341 ttccgccgcc tcccgcagcc cgagcgccgc agccagcgcc gcgttgacga cgcgcccatc
     8401 gcgcgtggcg acgccgcgcc gcagcgcggg gaggctgccg agcgccgcgt ccagaccctt
     8461 gtgggcgatc tcccagatga gcggccaggc cgcgatattc agcgcgtgcg tggccgtgcg
     8521 cgccaccacg gcggtcatat tcggcacgca gtagtggatc acgccctctt cgacgtatgt
     8581 cgggtcgcgg tgcgtcgtcg gccggctcgt ctcgacacag cccccctggt caatcgacac
     8641 gtccatgatc acggagcggg gccgcatcga ctgcaccatc tcgcgcgtga cgacgatcgg
     8701 cgcgcgcgcg ccaggctcga gcaccgcgcc gacgagcaca tcggcaaacc gcgccacctt
     8761 ccggatgttg aacgagtgcg acaccatcgt caccagcccg ccaccctggt gcatctcgtc
     8821 aagctgctgc aatcgccaca aatcgcgatc gagcacgtac acgctggcgc cggcgccgag
     8881 gaacgcgcgg gccgcggccg tgccgaacgt gccggcgccg atgatcacga cctcggcggg
     8941 cggcacaccg ggcacgccgc ccagcagaat gcccttcccg ccgcgatcgt tctgcagcag
     9001 cgtcgcggcc acctgcggcg ccatgcggcc ggcggcctgg ctgagtggct tgagcaccgg
     9061 cagcgatcca tcgtcgttct gtatcgtctc gtacgcgatg gccgtgacgc gcttatccag
     9121 cagaccctct acgcgctcgc ggcgaccggc tgccaggtgc aggaaaccca tcagaatgca
     9181 tccatcgcgc agccacccga cctcttccgc aataggccgg ctgaccttca ggacgagatc
     9241 ggcgcgtcca tatgcctctt cgggcgaata cacgattctc gccccggcgc gctcgtaatc
     9301 gacgtccgag aatcccgcac cccggccggc actcctttcg acgtaacagt gatggccgcc
     9361 cgccgtcagc aactcgacgc cagccggcgt caggccgacg cgatactcgt tcgggcggcg
     9421 ttcgcacggg atgccgatgt gcatgcgttt tctcctatga tcgggatgcg gaagaattcg
     9481 tcttcctggc gcgcaccagg agcacggggc agtcggcgtg ccgcgtcacc ttctcggcaa
     9541 cgctgccgta ggcccagcgc gccagccccg tgcgcccgtg ggtgcacatc gcaatcaggt
     9601 cggccctgac ttctgactgg agcgcgagga tctcctccgc cggctcgccg aaacgcacct
     9661 cgacgctcgt cgcaacgttg ccttgtgcca gggtcatcgc gacgccgttc aggtagttcc
     9721 gcagctccga ctcaatgcga ttgcgctcct gggtttgcgc ttcgaagtct gcccggctca
     9781 cgacgagcgg atacattctc agcggggcca ccgccgacac cggcgcagtc gccgacagcg
     9841 gggcgaccga aatcaggtgc actgtgaccg tgcgtccccg aaccaggtct cgaacgtacg
     9901 gtaggacctg ctcggccgtt tcggagccgt ccaatggaac gatgatgtta cggtacatgt
     9961 tggactccta aaacaagtga tcagtgatca gtcatcagtc atcagttgtt cggtcatcag
    10021 tgggggatga gcgtcggtat tgtacaacaa cttactgcca tcagtggcta ccgatcacca
    10081 attaccaatt accaattact gatcactgtt ctagccacct gacacttgag gtagctgaac
    10141 ctttgggaag ggccacagta agatcaggca gcgttcgatg aaatcagccc cgatccgaaa
    10201 caggcttaag tcgcggcggt cacggcgatc gacccagtga cgctgaccgg cgcggatgac
    10261 ggcgtgaccg atgaagagca gccagacgta caataggcac acggccaagg tcaagcgatt
    10321 cagccgcagg aaatgccgca ggtgagtagc ttccaggtca aagccatggt ctttcaagtc
    10381 gccgaacatt tcttcgatcc acatgcggcg cgcatacgcc cgtcgcgcgg cgtatcggtc
    10441 gggcagattg gtggccagca accacggttc gttttcgcct ttcgcccagt aggcgaacac
    10501 cgtggtgtga taggcccact gctgcgtcag gcgtgcctga ggccaccacg cccactggcg
    10561 cgggtgcgtc accaagtccg ccaaggcgtg ccaggtgcgg cgtcgatggg cggccacccg
    10621 gtgcgagcct ttttgacgca acacgtagct ccagccccag cgcgccaact gttccagcac
    10681 agaaatactg ccaaattcgg cgtctccgac cacgatcacc tgcgtgtcag tcggcaccag
    10741 gcggcgcacg taggcccaca gcgccaactg ggtcgccgcc gaactgtgac cacgctgacc
    10801 gcgcacccag caccaagcga tggggatggc ccgccgccga tacgcggccg ccaccatcag
    10861 cagttgatgt ccaaagccga cctggctggc gtccatgatc agcgtcacct tgtcgcccgc
    10921 ccacaggccc aacacctgtt gcgcggtcgc ccgataccac gtccgcacct cgaccgccgg
    10981 gttgtccaag aaccggctca gccgccgcgt caaactgacg actttggcca cgacgggcca
    11041 ctggcggacg atcgcgctgg catacacgct gcgcgccaag tacagaccga caatcagcca
    11101 cagcaggttg gtttgtcggg tgctgcactc atccggtatg tactcggcca agcgcgtgcg
    11161 ccaagtgcgg tacaatgtcg tcctgtccat cggtgcgcct cctgggggtt gtgtgtgcaa
    11221 ctcaacctta cccaggtttg ccccgatggg caacttttta c
//
LOCUS       DBNHXB010000341         4636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4117555, whole genome shotgun sequence.
ACCESSION   DBNHXB010000341 DBNHXB010000000
VERSION     DBNHXB010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4117555"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1342
                     /locus_tag="AC162G_13865"
     CDS             170..1342
                     /locus_tag="AC162G_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HIR5076938.1"
                     /translation="MSASPIDIVEAVTPQQRDEFIFFQWVPYKGNPYWVPPLISERRV
                     FLDKTKHPFHKHSEVALFLAKRAGQTVGTIAAIVNNRHNETHGENIGFFGLFECVEDY
                     AVAERLLATACDWVKARGKTSIRGPASYSVNDEIGLLVDSFDKPPCILMPYNPPTYKD
                     FIERFGFVKAMDLLQLFLDVGKLKDAERAMDPRLVRLTKKIQQRKKITLRPADLRRFN
                     EEVQIMKSLYAQAWEKNWGNVPMTEAEIDYLSHNLRQFVDPDLILIAEGDGVPVGLAL
                     CLPDMNIPLRKAYPRPGVPEWWTLIKLFYHWKVRKEIDTVRVVLLGVLEDYRKQGIEA
                     MLYMEVARRALPKGIHKAEGGWILEINDLMLKGAVSVGCEVFKRYRVYEMALFPSP"
     gene            complement(1384..2691)
                     /locus_tag="AC162G_13870"
     CDS             complement(1384..2691)
                     /locus_tag="AC162G_13870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5076939.1"
                     /translation="MLQPEAIDTTRSLNVMRRALFLSSFPIGVLTFAIPIFGPQVLKL
                     NAVRVGELFSVYALMTLIMRPVAGWAMDRYGRRNFFLAGLAVQVMASAFFAVGSSFDW
                     LFWGRVMQGIAAGMIWLSAYAMTADLAARGGRGNMFGAVEEMLARGGLYGVLVAVPFF
                     ARFSFNPFSFAFQIDAVGWTAIFVLYAALAAIAFYIAFRYLPETYVRRATQIKSDGSV
                     ISMQLIVLAVIVFLTSTALRGLEPILLIYVQDHFTTNLMIIALAYLPSALIFGFLQSR
                     LGRVADRVGRKLPIAVGLVTSGTSSAILPSLPGLVPMLGTWTMVPLVGLWASEAAAFS
                     AATPAEQALVADMSGGDKRGTAFGIYTLALSLGQVVGPLVGTQLYANVSPSAPFYLNT
                     VVLWLGALLVALLIRDPLRQRPVAASLSAREPPPPQWPASGGQ"
     gene            complement(2964..4358)
                     /locus_tag="AC162G_13875"
     CDS             complement(2964..4358)
                     /locus_tag="AC162G_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase type-2 family protein"
                     /protein_id="HIR5076940.1"
                     /translation="MTLSVDPVPLRHSLAAQRQLIDEAIRVALSAVDPREAIFRRVQR
                     VGHMLMIADRAIDLRAADRVLLVSIGKAAVPMADALIDLLGDDLSAGVVVTKYGHAAG
                     FEPPLAVQVVEGGHPVPDERSIAGARTIETILRAATSRDVLLCAISGGGSALVTLPIE
                     GVPLADLQATTDMLLRSGATIHELNAVRKHVDWFKGGGLARMSQGADAIALLLSDVVG
                     DDLSVIASGPTAPDPSTFQDAWRVVEQYRLAGVIPASVRAHLQAGLRGDVPDTPKPGD
                     PAFERVRNVIVGSNRAAAEAAQQFLHSRGLNTMLLSTFLEGEAREVGRVASAIAREIV
                     TSDRPVARPAALIAGGETTVTVRGSGAGGRNQELALSAAIGIDGLDDVWVVTLATDGA
                     DGPTGAAGAIVNGGTMACARRAGIDAGRRLAENDSHGFFNALSAAGDAACGGLIVTGP
                     TGTNVNDLLFVLAL"
     gene            complement(4371..>4636)
                     /locus_tag="AC162G_13880"
     CDS             complement(4371..>4636)
                     /locus_tag="AC162G_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HIR5076941.1"
                     /translation="TLSITTGQPQTLYLDEKDRALVDGRKIVLVDDVISTGSTLQGMR
                     LILEKAGGQIIATMAVFTEGERAKWKDIIALGHLPLFVDEAKV"
BASE COUNT          923 a         1527 c         1405 g          781 t
ORIGIN      
        1 cctgatggag ttccagacgg tcagaacccg acccaggtaa gtgttccccg cactgactga
       61 acaaatctcg caaacacgcc atcggcaagg ccccgatcga tccgatcggg gcgttttgct
      121 ttccgccgta cgcgacttat aatcggcgca tcacctccag ggggaatcca tgtccgcctc
      181 acccatcgac atcgtcgaag ccgtcacgcc gcagcagcgc gatgaattca tctttttcca
      241 gtgggtgccc tacaaaggca acccctactg ggtgccgccg ctcatcagcg agcggcgcgt
      301 ctttcttgac aagaccaaac accccttcca caagcacagc gaggtcgcgt tgttcctggc
      361 gaagcgcgcc gggcagaccg tcggcaccat cgccgcgatc gtcaacaatc gccacaacga
      421 gacgcacggc gagaacattg gcttcttcgg gttgttcgag tgcgtcgagg attacgccgt
      481 cgccgagcgg ctgctcgcga cggcgtgcga ctgggtgaag gcgcgcggca agacctccat
      541 ccgcggcccg gccagctatt cggtcaacga cgagatcggc ttgctcgtgg acagtttcga
      601 taagccgccg tgcatcttga tgccctacaa cccgcccacc tacaaggact tcatcgagcg
      661 cttcggtttc gttaaggcca tggacctgct ccagctattc ctcgacgtgg gcaagttgaa
      721 ggatgccgag agggcgatgg acccgcgcct ggtgcgtctg acgaaaaaga ttcagcaacg
      781 caagaagatc accttgcgcc cggcagatct caggcgcttc aacgaggaag tgcagattat
      841 gaagtcgctg tatgcgcagg catgggagaa gaactgggga aacgtgccca tgaccgaagc
      901 cgagatcgac tacctgagtc acaatctcag gcagttcgtt gaccccgacc tgattttgat
      961 cgctgaaggg gacggtgtgc cggtcggtct ggcgctgtgc ctgccggata tgaatatccc
     1021 attgcgcaag gcctatcccc ggcctggcgt gccggagtgg tggacgctga tcaagctctt
     1081 ctatcactgg aaagtgcgca aggagatcga cacggtgcga gtggtgttac tcggtgtgct
     1141 ggaagactat cggaagcagg ggatcgaagc gatgctctac atggaggtcg cgcgccgggc
     1201 gctgcccaag gggattcaca aagcggaagg cggctggatc ttagaaataa acgatctcat
     1261 gctgaagggc gcagtgtctg taggctgcga agttttcaag cgataccgcg tttacgagat
     1321 ggctctcttc ccatcccctt gacctgtgcg agacatctcc agatgccgag gtcgtggcga
     1381 gagctattgc cctccgctcg ccggccactg tggcggaggc ggctcgcggg ccgacaaact
     1441 tgccgcaacg ggccgctgcc gcaacggatc gcggatcaac aaagccacca ggagcgcgcc
     1501 gagccacagc acgaccgtgt tgagatagaa aggcgcgctc ggcgaaacat tcgcgtagag
     1561 ctgtgtgccg accagcggcc ccacgacctg ccccaggctc aaggccagcg tataaatgcc
     1621 gaaggccgtg ccgcgcttgt ctccgccgct catgtcggcg acgagtgcct gctcggcggg
     1681 cgtggccgcc gagaaagccg ccgcctcgct ggcccatagc ccgaccagcg gcaccatcgt
     1741 ccacgtaccc agcatgggca cgagaccggg caggctgggc agaatcgcgc tcgacgtgcc
     1801 gctggtcacc aacccgacgg cgatcggcag cttgcgaccc actcgatcgg ccaccctgcc
     1861 caggcgcgac tgcaggaaac cgaagatcag cgccgacggc aaatacgcca gtgcgatgat
     1921 catcagattc gtcgtgaaat ggtcttgcac gtagatcagc agaataggct ccaggccgcg
     1981 cagcgccgtg gaggtcagga acacgatcac ggccaggaca atcagttgca tggaaatcac
     2041 cgagccgtcg cttttgatct gcgtcgcccg ccgcacgtac gtttcaggca ggtagcggaa
     2101 ggcaatgtag aaggctatcg cagcgagcgc cgcatacagg acgaatatcg ccgtccagcc
     2161 cacggcgtcg atctggaacg caaaactgaa ggggttgaac gagaaccgcg cgaagaaggg
     2221 cacggcgacg agcacgccat acagcccgcc gcgcgccagc atctcctcga ccgcgccgaa
     2281 catgttgccg cgcccgccgc gcgccgccag gtcggcggtc atggcgtagg ccgagagcca
     2341 aatcatgccc gcggcgatgc cctgcatcac gcgtccccag aacagccagt cgaacgagct
     2401 gcctacagcg aagaacgcac tggccatcac ctgtacggcc aggccggcga ggaagaaatt
     2461 gcggcgcccg taccgatcca tcgcccagcc cgcgaccggc cgcatgatca acgtcatcaa
     2521 agcgtagacg ctgaacagct ctcccacccg tacggcgttg agtttgagca cctgcggtcc
     2581 gaatatcgga atcgcaaacg tcagcacgcc gatcggaaac gatgacagaa acaaggcgcg
     2641 gcgcatgacg ttgagcgagc gtgtggtatc gatcgcttcg ggttgaagca tccaattctc
     2701 ccgcacggcc ggggcgtgat ccgtcaacat cacacgggga ggtaaaacct ccccgtgcca
     2761 gcgccattat aacacaccaa ctccccggcg caacgtgacc tgagaggcat tcttcggaac
     2821 gtgccccgat cactcgccga tgtcggagcc gctggagttc tccaggcgcc cgctacgccc
     2881 tcgccctagc cgagcagcgc gtcgacccgc ttcggatcgg acttgttcct ggacaccgtt
     2941 cgcttcaggt atgggcagcg acctcataat gcaagcacga acagcaggtc gttgacattc
     3001 gtgccggtcg ggccggtgac gatcaaccct ccacaagcgg cgtctccggc cgcggacagt
     3061 gcgttaaaaa atccgtgtga atcgttctcc gccaggcgcc tgcccgcgtc gattcccgcc
     3121 cgtcgcgcgc aggccattgt accaccgttg acgatcgcgc cggcggcgcc ggttggaccg
     3181 tcggctccgt cggtcgccaa cgtgacgacc cacacatcgt ccagtccgtc gatgccaatg
     3241 gctgccgaca gcgccagctc ctggttgcgt ccgcccgcac cgctgccacg tacagtgacg
     3301 gtcgtctcgc cgccggcaat gagtgcagca ggccgcgcaa ccggacgatc gctcgtgacg
     3361 atctctcgcg caatagcaga ggcgactctg cccacttcac gtgcttcacc ctcaaggaat
     3421 gtagataata gcatggtgtt gagtccgcgc gaatggagaa actgctgcgc agcctcggcc
     3481 gccgcgcgat tgctgccgac gatcacgttg cgcacgcgct cgaacgccgg gtcgcccggc
     3541 ttgggcgtat cgggcacatc gccgcgcaga ccggcctgca gatgggcgcg caccgaagcg
     3601 gggataacgc ctgccaatcg atattgctcg accacgcgcc acgcatcctg aaacgtagac
     3661 ggatccggcg cagtcgggcc agaggcgatg accgagagat catcgccgac gacgtcgctg
     3721 agtaagagcg caattgcgtc tgcgccctgg ctcatcctgg ccagtccccc acccttgaac
     3781 caatccacgt gcttgcgcac cgcgttcaac tcgtgtatcg tcgcgccgga gcgcaagagc
     3841 atatccgtcg tcgcctgcag gtcggccagc ggcacgcctt cgatcggcag cgtgaccagc
     3901 gccgagccgc cgcccgatat ggcgcacagc agcacgtcgc ggctcgtcgc agcgcgcagt
     3961 atcgtttcga tcgtgcgtgc accggcgatc gagcgctcat ccggcaccgg atgcccgcct
     4021 tccaccacct gcaccgccag cggcggctca aagcccgccg cgtggccgta cttggtgacg
     4081 acgacgcctg ccgacagatc gtcgccgagc agatcgatca gcgcatcggc catcggcacg
     4141 gcggcttttc cgatcgagac gagtaaaacc cgatcggcgg cgcgcagatc gatggcgcga
     4201 tcggcgatca tcagcatgtg ccccacacgc tgcacacgcc ggaagatcgc ttctcgcgga
     4261 tcgacggcgc tcagcgccac gcggatcgct tcgtcgatga gttgtcgctg agctgccagg
     4321 ctgtggcgca gagggacggg atcgaccgaa agcgtcatcg tgctttagag ttacactttc
     4381 gcctcatcca cgaacagcgg caggtgcccc agcgcgatga tgtccttcca cttggcgcgc
     4441 tcgccttctg tgaacaccgc catcgtcgcg atgatctgcc cgccggcctt ttcgaggatc
     4501 aggcgcatgc cctgcagcgt gctgccggtg ctgatcacgt cgtcgaccag cacgatcttc
     4561 ctgccgtcga cgagcgcccg atccttctcg tcgaggtaga gcgtctgcgg ctggccggta
     4621 gtgatgctga gcgtct
//
LOCUS       DBNHXB010000342         3994 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2608106, whole genome shotgun sequence.
ACCESSION   DBNHXB010000342 DBNHXB010000000
VERSION     DBNHXB010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2608106"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="AC162G_13885"
     CDS             complement(<1..637)
                     /locus_tag="AC162G_13885"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5076942.1"
                     /translation="MPLAADILLVHARCFTADPANPRAEAVAVCGNRIVFVGSDVDAA
                     AWRGPGTRVIDAQGCTLMPGFIDSHFHLLLGSIGLGDAQLGDVRSYGALSEAIRAFAG
                     EHSRREWLVGHGLPYAILPGGRMLTRHDLDAIIVDRPLIVFAYDTHTAWANTEALRRA
                     SLLENGKTVAPNSEIVLGDDGLASGELREPGAYQPVLDLIPEPNDVEKRALL"
     gene            798..2225
                     /locus_tag="AC162G_13890"
     CDS             798..2225
                     /locus_tag="AC162G_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076943.1"
                     /translation="MTVHVQRSRSRFFITLVVLSLLLALDTLTLHFTQSGESEAGAKG
                     KRRSGVVLSEVVAASAVQPARAPSPPRSGFAPQTRLGFTTGDQWEPSIAADRFGHVYV
                     LYPQYLGVPGCEECFSPTMILQISSDRGATWTSPELMHPAGATTGQWDAQIVVDPIDG
                     RTVYAAWLQNSKSDTVVAKSTDFGETWSVVVANSTNAATDKPILAVRGQDVYVGYNQA
                     QKIWVSASHDGGATFTSVNANRTHTGQLGWSLAGGGTVDPAGNVYFAWDGYEQNGGAK
                     GPVNLYISKSSDGGATWQTILIDVSGSPPDCSDFLCGWAYLGAQITMASDAAGTLYTL
                     WNAGEVDKGPERIYFAKSTDAGQTWSPRSDISAAPDGVNHAFPAIAARADGDVRISWM
                     DARADGLWNTYYRSSTNGGATWSAEADLSTLVAGFSYIFPDGFSFPFGDYFEIDIDDR
                     GTTHAVWGEGLNYDSPGSIWYTRGK"
     gene            2433..3452
                     /locus_tag="AC162G_13895"
     CDS             2433..3452
                     /locus_tag="AC162G_13895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5076944.1"
                     /translation="MPESVDYDVVVVGSGPAGASTARAAAQRGLRVLLVDKKQELGAP
                     IQCSGAVSAHALRDADIIPIDEFVASDIYGFAVYDAQGDVTTVDYRALKPDVYGQAPL
                     GYVVDRRRFDRHLAMLAERAGAQVWLKTEALGHSPQENGFTQVRLKRFGQPTAVRARV
                     IVGADGVQSQVGKWADLSTHIRLSELASCLQLIVDGVECNGLLEIITGHAWAPGGYAW
                     VFPKGHSYAEVGLGVIRTLTDKDARWHLDHFMRHSFLADRLEACRVLEVQGGGVPLAA
                     PLKRPYADHIILVGDAARHVNPITGGGIHTALRSGVIAGQFLADLLSAGERPLAAALG
                     GYQQR"
     gene            3474..3599
                     /locus_tag="AC162G_13900"
     CDS             3474..3599
                     /locus_tag="AC162G_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076945.1"
                     /translation="MGKLHQMKTTIFRQKDIARRDQILYQTLAGYFRPGSEFRKV"
     gene            3608..3835
                     /locus_tag="AC162G_13905"
     CDS             3608..3835
                     /locus_tag="AC162G_13905"
                     /inference="COORDINATES: protein motif:HMM:NF024207.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR5076946.1"
                     /translation="MPKPHPVVFSVDWNACIRCGACVAVCPQAPGFTTPFDTIAVDTP
                     CDIACMACEKICPVTAIAWRAAMLQAPIPVG"
BASE COUNT          725 a         1268 c         1294 g          707 t
ORIGIN      
        1 gcagcagcgc ccgtttctcg acatcgtttg gctcgggaat gagatcgagc accggctgat
       61 aggcgcccgg ctcgcgcagc tcgccgctcg ccagcccgtc gtcgccgagg acaatctcgc
      121 tgttgggcgc aaccgttttg ccgttctcca gcaggctcgc gcggcgcagc gcttcggtat
      181 tggcccacgc cgtgtgtgtg tcgtaggcga agacgatcag cgggcgatcg acgataatcg
      241 cgtcgaggtc gtgccgcgtg agcatccggc cgccaggtag aattgcgtac gggaggccgt
      301 gcccgacgag ccactcgcgc cgcgaatgct caccggcgaa ggcgcggatc gcttcgctaa
      361 gcgcgccata acttctaacg tctccgagtt gtgcgtcgcc caatccaatc gagcctagga
      421 gcaggtgaaa gtggctgtcg atgaagccgg gcatgagcgt gcagccttgc gcgtcgatca
      481 cgcgcgtgcc ggggccgcgc caggccgcgg catcgacatc gctacccacg aacacgatac
      541 gattgccgca cacggcgacg gcttcggcgc gcgggttggc cgggtcggca gtgaagcatc
      601 gggcatgcac gaggaggatg tcagcggcaa ggggcatatg agtgcgacct ccgaaatgga
      661 atacgggcaa gtatagccgg gagtggatga accggcgagt cagcaatgga catacgattg
      721 gcgcgcacgg ccgagcattt ccccatacga cgctaactgg tgtagaatct gttcccatca
      781 cctacgagga gggcgccatg actgtccatg tccaacgatc acgctcaagg ttttttatta
      841 cgctcgttgt actatcgttg ctgcttgcgc tcgacacgct gacgctgcac ttcactcaaa
      901 gcggcgaatc cgaagcgggc gcaaagggca aacgccgcag tggcgtcgtg ttaagcgaag
      961 tcgtcgccgc ttcggctgtg cagccggcga gagcgccttc gccgccaagg tccggcttcg
     1021 cgccgcaaac acggctcggg ttcaccactg gcgaccagtg ggaaccgtcc atcgccgccg
     1081 accgtttcgg ccacgtctac gtgctgtatc cgcagtacct gggcgtgccc ggttgtgaag
     1141 aatgcttcag cccgacgatg atcctccaga tcagcagcga tcggggcgca acctggacct
     1201 cgcccgagtt gatgcacccg gcgggcgcga cgacaggcca gtgggatgcg caaatcgtcg
     1261 tggatccgat cgacggtcgc acggtgtacg ccgcatggct gcagaacagc aagagcgaca
     1321 ccgtcgtcgc caagtcgacc gacttcggcg agacctggtc ggtcgtcgtc gcgaacagca
     1381 ccaacgcggc gaccgataag ccaattctcg ccgtgcgcgg ccaagatgtg tacgtcggct
     1441 acaaccaggc gcagaaaatc tgggtgtcgg cctcgcacga cggcggcgcg acctttacgt
     1501 cggttaacgc gaacagaacc cacaccggcc agctaggctg gtcgttggcc ggcggcggca
     1561 cggtcgatcc ggccggtaac gtgtacttcg cgtgggatgg ttacgagcag aacggcggcg
     1621 ctaagggacc ggtgaatcta tacatcagca agtcctcaga tggcggcgcc acgtggcaga
     1681 ccatcctgat cgacgtgtcc ggctcgccac ccgactgctc ggactttctg tgcggctggg
     1741 cctacctcgg cgcgcagatc acgatggcct ccgacgcagc cggcacactg tataccctgt
     1801 ggaacgcggg cgaagtggac aaaggcccgg agcgcatcta cttcgccaag tcgaccgacg
     1861 ccggccaaac ctggtcgccc cggtcggata tttcggctgc cccggatggg gtcaatcacg
     1921 ccttcccggc catcgccgcc agggcggacg gcgatgtgcg catctcgtgg atggacgcgc
     1981 gcgcagacgg gttgtggaac acctactacc gcagctcgac gaacggcggc gcaacctggt
     2041 cggccgaagc cgatttgtcc acgctcgtcg ccggcttcag ctacatcttt ccggacggct
     2101 tcagcttccc gttcggcgac tacttcgaga tcgacatcga cgatcgggga acgacacacg
     2161 cggtctgggg cgaagggctg aactacgaca gcccgggatc aatctggtac acgaggggca
     2221 agtagacctc acccccacct ccagccggca ttcgctcgac ccgctgcaac tgccgcgcat
     2281 ggcaatggcc gacttcgcca ggcaagcgcg cgatataggc gtgaactaca tcggctcgtg
     2341 ctgcggttcc gtcgccgcgc acgttacgtg atacgtaaaa cgtgatgcgt gatgggtgag
     2401 tgggaagata aagttttcag caggagggcc atgtgcctga gtcggtcgat tatgatgtgg
     2461 tcgtggtcgg gtcggggcct gcgggagcca gcacggcgcg cgctgccgcg cagcgcggct
     2521 tacgcgtgct gctggtcgac aagaagcagg agctgggcgc gccgatccaa tgctcgggcg
     2581 cggtcagcgc ccacgccctc cgcgatgcag acatcattcc tatcgacgag ttcgtcgcca
     2641 gcgacatcta cggctttgcc gtctacgatg cgcaaggcga cgtcacgacc gtcgactacc
     2701 gcgccctgaa acccgacgta tacggccagg cgcccctggg ctacgtcgtc gatcgccggc
     2761 gcttcgatcg tcacctggcg atgctggccg agcgagccgg cgcacaggtc tggctgaaga
     2821 ccgaggcgct cggccacagc ccacaggaaa acggcttcac ccaggtgcgc ctcaagcgct
     2881 tcgggcaacc gacggcggtg cgtgcgcgcg tcatcgtcgg ggccgatggc gtgcaatcgc
     2941 aggtaggcaa atgggctgac ctgtccacgc acatcaggct cagcgagctg gcttcgtgcc
     3001 tgcagctcat cgtcgatggc gtcgagtgca acgggctgct ggagatcatc accggtcacg
     3061 cgtgggcgcc cggtggctat gcctgggtct tccccaaggg gcacagctac gccgaagtgg
     3121 ggctgggcgt gattcgcaca ctgacggaca aagatgcgcg ctggcatctg gatcatttta
     3181 tgcgtcactc ctttctcgcc gatcggctcg aggcatgccg cgtgctggaa gtgcaaggtg
     3241 ggggcgtgcc gctggcggct ccgctcaaac gaccgtatgc cgatcacatc atcctggtcg
     3301 gcgatgccgc gcgccatgtc aacccgatca cgggcggtgg catccacacc gcgctgcgca
     3361 gtggcgtgat tgccgggcaa ttcctggccg acctgctatc cgccggtgag cggccgctcg
     3421 ccgccgcgct cggcggctat cagcagcgat gactggagga gttcggccat gcgatgggta
     3481 agttgcacca gatgaagaca accatcttcc ggcagaagga catcgcgcgc cgcgaccaga
     3541 tactgtacca aacgctggcc ggttatttcc gtcctggctc agagttccgc aaagtgtaaa
     3601 cgatccaatg cccaagccac atccggtcgt cttctctgtg gactggaacg cgtgcattcg
     3661 atgcggagcg tgcgtggcgg tgtgccctca ggcgcccggc ttcacgacgc cgttcgatac
     3721 cattgccgtg gacacgccgt gtgacatcgc ctgcatggcc tgcgagaaga tctgtcccgt
     3781 cacggcgatt gcgtggcgcg cagccatgct tcaggcgccg atcccggtgg gatgaaggcg
     3841 tgatcgggcg cgcgctcatc tggacagata tacttatcgc gagcagcgct gccggctggg
     3901 tggtcgttac ccttcgcgca ctcgctctgc cgacggattt cagcctgatc gccctgggct
     3961 ttatcttagc actcgtcttt tacacacgcg accg
//
LOCUS       DBNHXB010000343         4866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1266387, whole genome shotgun sequence.
ACCESSION   DBNHXB010000343 DBNHXB010000000
VERSION     DBNHXB010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1266387"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..497
                     /locus_tag="AC162G_13910"
     CDS             240..497
                     /locus_tag="AC162G_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HIR5076947.1"
                     /translation="MSSISVLEIPQDVLDSARLSIGDIKVELAVSLYAQQRLSIGKAR
                     ELADMSLWEFRQLLAARRISPHYDEIELAEDIATLKKLGRL"
     gene            494..979
                     /locus_tag="AC162G_13915"
     CDS             494..979
                     /locus_tag="AC162G_13915"
                     /inference="COORDINATES: protein motif:HMM:NF023276.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR5076948.1"
                     /translation="MTVVSNTSPLTSLAAIGQFELLRRLYGEVHIANAVWDELNAGGR
                     SWPGRDEVASASWVQRREVKNHLLVTAFQRDLDRGEAETITLALEVDADLVLLDEREG
                     RHVAQRLGLQVVGVVGILLEAKAKGMIDRMKPNLDALRQAAGFHLSETLYNSALRASG
                     E"
     gene            1191..2606
                     /locus_tag="AC162G_13920"
     CDS             1191..2606
                     /locus_tag="AC162G_13920"
                     /inference="COORDINATES: protein motif:HMM:NF009883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5076949.1"
                     /translation="MDLFDHALKQRLATEAPLAARMRPRTLDEFVGQEDIVGAGRLLR
                     RAIEADRLFSSIILWGPPGTGKTTLAQIIANSTKSHFETISAVLAGIPELRRVIGEAQ
                     ERRKLHGIRTTLFVDEVHRWNKAQQDALLPHVENGTVVLIGATTENPYFEVISALVSR
                     SRIFQLKALSDDDVQQILLNALTDPERGYGARSVHVDDNALAHLVHIAGGDARNALNA
                     LELAVESTLPGVDGAIRVTLEVAQESIQRRAILYDKDGDVHYDTISAFIKSVRGSDPD
                     AALYWLAKMLYAGESPRFILRRLLILAGEDIGLADPMGLVVANAAAQAFEYVGLPEGV
                     YPIVEATLYLATAPKSNSAGAYFKAYAKVEEEGKVDVPKHLQDSSRDAKSLGHGEGYV
                     YPHELPGHHVGQQYLPTALLGTYFYDPSAEGHEAQVKERLARWRAAQEKALGISETQT
                     LADLSVPEIVAIKQKIAKRAG"
     gene            2663..4438
                     /locus_tag="AC162G_13925"
     CDS             2663..4438
                     /locus_tag="AC162G_13925"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR5076950.1"
                     /translation="MGKRTAFGLLLLGLAGLGLYAYRRQMIARMLGLRPPQYATGVER
                     NIRVPMPDGVGLMTDHYFPKAAGRLPAILIRSYYGRGYDVGSLLGWLMSFLSVRFAER
                     GYHVVVQTTRGRFDSEGETEPFVDAVPDGHATLDWIARQPWCDGKVGMWGPSYLGYVQ
                     WAVAADPQRSPLLKAIVPQMIGSRVPNIVYPNGAFALDTFVRWVFAVDVLDNLKKRSN
                     AESLRRINPQGMARYLAPAFRHLPVGESDTVSLGRPLTYFRNWLAHLDPNDPHWQQMD
                     LHPHLPQVTAATHLIGGWYDIALRETIDDYAALKAAGRSPYLTIGPWTHGERPSIVAA
                     LCEGIAWFDAHLKGDRRGLRKKPVRICVMGTDRGAWREMDDWPPPARETRYFLRSEGR
                     LETSEVFETSEVSPPDRYRYDPADPTPSVGGPLLMPPSGPMDNRALEARPDVLTYTTP
                     ALKRDVEVIGPVRLELFVQSSLAHTDFFGRLCDVHPDGRSINICDGLFRIEPEGGAVG
                     RPAPARQPDGVLRIEVDMWATAHRFRRGHRLRLQVSSGAHPRWSRNLGTGEPIATGTK
                     MLVADQAVYHDGDHPSALVLPIVAE"
BASE COUNT          920 a         1496 c         1560 g          890 t
ORIGIN      
        1 tccgggtatt caaccgtgag tccgagttgt tccagaacgg ccacgacggc catgcccgcc
       61 tgcgggcgca cagcctcgac gagacaggta gcgaggagct ggacggcagt gatcatgaga
      121 gtcaggagcg agggtcagga gttagagttg ggagttagag tgtaattcta ctccgaaatg
      181 agagaagtct ccaaatgcgg tataatccac tgtgagcctt atgaaatgag gagcggacga
      241 tgagttccat cagcgtgcta gagattccgc aggatgtgct cgattcggcc cgtctgagca
      301 tcggtgacat caaggttgaa ctggccgtct cgctgtacgc ccaacagcgg ctgtcgatcg
      361 gcaaggcgcg tgagctggcc gacatgtcgc tgtgggagtt tcggcaattg ctggccgccc
      421 gccgtatttc gccgcactac gatgagatag agctggctga agatatcgcc accctaaaga
      481 aacttgggcg actatgacgg tcgtcagcaa cacctcgccc ttgaccagcc ttgcagctat
      541 cggccagttc gagctattgc gccggctgta cggtgaagtg catatcgcaa acgccgtatg
      601 ggacgagttg aacgctggcg ggcgaagctg gcccgggcgc gacgaggtcg cctctgccag
      661 ttgggttcaa cgccgcgaag tgaagaatca tctgcttgtt actgcctttc agcgtgacct
      721 cgatcgcggc gaagcagaaa ctatcacgct ggcgctcgaa gtcgatgcag acctggttct
      781 tctggatgag cgcgaaggtc ggcatgtggc tcaacgcctg gggctgcaag tggtaggcgt
      841 agtcggcatc ttgctggaag ccaaggccaa aggcatgatt gatcgcatga aaccgaacct
      901 ggatgcgcta cgccaggcag ccggattcca cctgagtgaa acactctaca attctgcact
      961 gcgagccagc ggggaatagc gctatcggat ccaggacaat gtaggtccgc gtggtcgccc
     1021 caggtcaatc cgccaaattc acccacgcaa atccagataa gccttttctg cccacccgtt
     1081 ggggcgacag ttgcactgtc gccgggcgtc tgcagtgcaa ctgcggacga tcgagttggc
     1141 aggtaattcc agcgtgatct cacttcccgc attcgtgcta tactgattgc atggatctgt
     1201 tcgatcacgc tcttaagcag cgcctggcga cggaagcgcc gctcgccgcg cgcatgcgcc
     1261 cgcgcacgct cgacgaattc gtcgggcagg aagacatcgt cggggcgggg cggctgctgc
     1321 gccgcgcgat cgaggccgac cggctgttct cgtcgatcat cctgtggggg ccacccggca
     1381 cgggcaagac gacgctcgcg cagatcatcg ccaactcgac gaagtcgcac ttcgagacga
     1441 tcagcgcggt gctggccggc atccccgagc tgcgccgcgt gatcggtgaa gcccaggaac
     1501 gtcgaaagct gcacggaata cgcacgacgc tcttcgtcga cgaagtccat cgctggaaca
     1561 aagctcaaca agacgcgtta ctcccccacg tcgaaaacgg caccgtcgtc ctgatcggcg
     1621 cgacgaccga gaacccttac ttcgaggtga tctcggcgct cgtctcgcgc tcgcgcatct
     1681 tccagctcaa ggcgctgagc gacgacgatg tgcagcagat tcttctgaat gcgctgaccg
     1741 atcctgaacg aggctacggc gcacgcagcg tgcacgtcga cgacaacgcg ctggcgcacc
     1801 tggtccacat cgccggcggc gacgcgcgca atgcgttgaa cgcgctcgag ctggccgtcg
     1861 aatcgacact gcccggcgtg gacggtgcga tccgcgtcac gctcgaagtc gcgcaggagt
     1921 cgatccagcg gcgcgccatc ctttacgaca aagacggcga tgtgcactac gacacgatca
     1981 gcgcgttcat caagtcggtg cgcggatcgg accccgacgc ggcgctgtac tggctggcga
     2041 agatgctgta cgcgggcgag agtccgcgct tcattctgcg ccggctgttg atcctcgcag
     2101 gcgaagacat cggcctggcc gatccgatgg ggctggtcgt cgcgaacgcc gccgcgcagg
     2161 cgttcgagta cgtcggcctg ccggaaggcg tttacccgat cgtcgaggcg acgctgtatc
     2221 tggcgaccgc gcccaagtcg aacagcgcgg gcgcgtactt caaggcatat gcgaaggttg
     2281 aagaagaagg caaggtcgac gtgccgaagc acttgcaaga cagtagtcgt gacgcgaaga
     2341 gcctcggcca cggcgaaggc tacgtttatc cccacgagct gccggggcat cacgtcgggc
     2401 agcagtatct ccccaccgcg ctgctcggca cgtatttcta cgatccctcc gccgaaggcc
     2461 acgaagcgca agtgaaagag cgcctcgcgc gctggcgcgc ggcgcaggag aaggcgctcg
     2521 gcatcagcga gacgcagacg ctggctgact tgtctgtgcc tgagatcgtc gccatcaagc
     2581 agaagatcgc gaagagagcg ggatgaccgt tcagacttcg gaagtctcta aaagacttcc
     2641 gaagtcttca ggaggctgcg cattgggcaa gcgaactgca tttggccttc tgctgttagg
     2701 cctggccggg ctgggcctat acgcctaccg ccgccagatg atcgcgcgga tgctcggcct
     2761 ccggccgccg caatacgcga cgggcgtcga gcggaatatc cgcgtgccga tgcccgacgg
     2821 cgtcgggctg atgaccgacc actacttccc caaggccgcc gggcgcttac cggcgatact
     2881 gatccgctct tactacgggc gcggctacga cgtgggcagc ctgctcggct ggctgatgtc
     2941 attcttgagc gtgcgcttcg ccgagcgcgg ctatcacgtc gtcgtccaga cgacgcgcgg
     3001 ccgtttcgat tccgaaggtg aaaccgagcc attcgtcgac gccgtgccgg acggacacgc
     3061 cacgctcgat tggatcgccc gccagccgtg gtgcgatggc aaagtcggga tgtgggggcc
     3121 gagttatctg ggctacgtgc agtgggccgt cgccgccgat ccgcagcgct caccgctgct
     3181 caaggcgatc gtgccgcaga tgatcggttc gcgcgtgccc aatattgtct atcccaacgg
     3241 ggcgttcgcg ctggacacct ttgtgcgctg ggtctttgcg gtcgacgtgc tggataacct
     3301 gaagaagcgg tcaaacgcgg agagcctgcg gcgcataaac ccgcagggca tggcgcgcta
     3361 cctcgcgcca gcgtttcgac acctgcccgt gggagagagc gacacggttt cgctgggtcg
     3421 tccacttacc tacttccgga attggctcgc ccacctggat ccaaacgatc cccactggca
     3481 gcaaatggat ctccatccac acctgccgca agtgacggcg gcgacgcatc tgatcggcgg
     3541 ctggtacgac atcgccctgc gtgagacgat cgacgattac gccgcgctga aggccgccgg
     3601 acgctcgccc tatttgacga tcggcccgtg gacacatggc gagcgtccga gcatcgtcgc
     3661 ggcgctatgc gagggaatcg cctggtttga cgctcacctc aaaggcgatc gtcgcgggct
     3721 gcgaaaaaag ccggtgcgga tttgcgtgat gggtaccgat cgcggagcgt ggcgtgagat
     3781 ggacgactgg ccgccgcctg cgcgggagac gcgctacttt ctgcgctccg agggacgatt
     3841 ggagacttcc gaagtcttcg agacttcgga agtctcgcca cctgatcgtt atcgctatga
     3901 cccggccgat cccacgccgt ccgtcggcgg gccgctgctc atgccgccct ccggcccgat
     3961 ggataaccgg gcgctcgaag cccgacccga tgtgctgacg tacacgacgc ctgcgctgaa
     4021 gcgcgatgtc gaagtgatcg gccccgttcg gctggagttg ttcgtgcaat cgagcctggc
     4081 gcacaccgac ttcttcggcc ggctgtgtga cgtgcacccg gacggcaggt cgatcaacat
     4141 ctgcgacggg ctgttccgca tcgagccgga agggggcgcg gtcgggagac cggccccagc
     4201 ccggcagccg gacggcgtct tgcgcatcga agtcgacatg tgggcgacgg cgcatcgctt
     4261 ccggcgcggt caccgcctgc ggttgcaggt ctcctccggt gcgcatccgc gctggagccg
     4321 caacctgggg acgggcgagc cgatcgcgac cggcacgaag atgctcgtcg cggatcaggc
     4381 cgtgtatcac gacggcgacc atccttcggc gctcgtcctg ccgattgtcg cggaatgatc
     4441 cttcgcaggg cagcggggac agacttcgga agtctttaag gatagcctcg ccagcgggac
     4501 aacgccggtc gacggactcc ctcgcctgcc agatcacgcc tgagacttcc gaagtctttg
     4561 cgctaatcga gtggtctgcg catggtccaa gcgtgctggc cgtgcctgcc tcttgggcca
     4621 ctgtcttcct gaatcgacac ggttgctgac acggcgactt ggcttaattt ccaagttgct
     4681 ctaaacttga gacgtcgtcg gacgtggtgg ggcgacacta cgggcggctt cgttcaactc
     4741 aggatgatgt gtcgcggagc cgccacatca atcgttattt gttgggcgcg gactcccgtc
     4801 gcagacgacc gttgcacaca tgaagaggtc atcgaaatca tgagtgatat cgagaaccca
     4861 gctgag
//
LOCUS       DBNHXB010000344         6100 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2944516, whole genome shotgun sequence.
ACCESSION   DBNHXB010000344 DBNHXB010000000
VERSION     DBNHXB010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2944516"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1671)
                     /locus_tag="AC162G_13930"
     CDS             complement(<1..1671)
                     /locus_tag="AC162G_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HIR5076951.1"
                     /translation="MAKTFDQSLDEIARLVKHYQTNRTAFHAPGYKEAHARQNLIDPL
                     FIALDWDVYNAERAAPQYQPVVLEDSLETEMGKKAPDYAFRIGRDPAFFAEAKKPAES
                     IKTNPDHAYQLRRYAWSAKLPLSILTDFEELAVYDCRARPSDKDKVSVGRINYLTFEE
                     YPDRWRELWDVFSYGAVRSGSFDAYAQTAKGKRGTGEVDAEFLKEIEHWRDVLARNIA
                     LRNPRLTIDELNDAVQRTIDRLIFLRMAEDRGIEPYGKLLALTPTPSAASPVGKASTG
                     EGSVYAGLIDLCRAADAKYNAGLFDFSSKGDALALKLKVDDGVLKPILAGLYYPQSPY
                     EFSVLPAEILGSVYEQFLGKVIRLTPSHHAKVEEKPEVKKAGGVYYTPAYIVEYIVKN
                     TVGKLVEGKAPKQLEGFCVLDMACGSGSFLLGAYEYLLDYYLQWHIANSVSPMATGDK
                     RSVISRSVPIYQAPDGSWRLTTAEKKRILTAHIFGVDIDQQAAEVTKLSLLLKVLEGE
                     SDETLGRQLALFPERALPNLDANIKCGNSLIGPDYFSDQLMPDTEELRR"
     gene            complement(1790..2173)
                     /locus_tag="AC162G_13935"
     CDS             complement(1790..2173)
                     /locus_tag="AC162G_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006669832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR5076952.1"
                     /translation="MILLDTDVMIDVLRKHPPAVAWLQASLTEELGLPGLVVMELLQG
                     CRTIDEQQRVEALLHPYTLYWPTQTDCARALADFTAYHLSHNLGLLDSLIAETAIGLG
                     ITLATFNEKHYSALSALKTAQPYSR"
     gene            complement(2160..2435)
                     /locus_tag="AC162G_13940"
     CDS             complement(2160..2435)
                     /locus_tag="AC162G_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076953.1"
                     /translation="MMTLAVRTKTIVQPGGLVEIRSPELTPGASAEVIVLVDAPAPQR
                     VMTAANLLDSGLVGLWEDRTDIADSAEFARQLRRQAERRERAGDDPA"
     gene            2628..4109
                     /locus_tag="AC162G_13945"
     CDS             2628..4109
                     /locus_tag="AC162G_13945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5076954.1"
                     /translation="MRRPGLASPPSLAQYALAVLSTVTATVVLLILRDSLSTPIVALL
                     YLLPVVFSTATWGFGPGVVAALCAFLSFNFLFIVPYYTFAVHQAHDVLALVVFLIVAV
                     VISRLLGRARAGVTAATAREQETASLYELSMALAGLQDEQAIARTIAEHVLETFQAEH
                     VELLIKGKVGHFRVSLPEPPAPLSDQPMCMTPLETARGRQGEIRLWRGEPPIAPVEER
                     LLRTFASQGALALERARLAQAETRAKVLEESDRLKSALLSSVSHELRSPLAAIKAGIT
                     SLRSREVHWDSPAGADLLAVVEEEVDHLNRLVGNLLDMSRIESGALKPKRQWNVLVDI
                     FGSALERMHRAAEAYRIEIDLRDDLPLVPVDAAQLEQVFTNLISNSLKYAPPGSVIYI
                     GACPQDDQTLLVEMRNQGPPIDEEHLERIFDKFYRVTAADRVTGTGLGLSICKGIVEA
                     HGGRIWAENRPDGLAFKFTLPLTWDGAQPPVLPPTVLSESESS"
     gene            4106..4801
                     /locus_tag="AC162G_13950"
     CDS             4106..4801
                     /locus_tag="AC162G_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018885986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5076955.1"
                     /translation="MSTQPHILVIDDEPQILRAMRTILTEKQFRVTTASRGEEGLALA
                     AANPPDVVILDLGLPDVDGIEVCARLREWTHIPIIVLSVRDREQDKVAALDKGADDYL
                     TKPFAVQELLARVRVALRHLTQAKGSPKTVVTAGPIAIDLTRHVVTREGVEVKLTATE
                     FNLLAYLATNAGRLLTHESILTHVWGPEAAGRAEYLRVFIGQLRKKLEADPRRPRMIL
                     NEPGIGYRFVADD"
     gene            5169..5894
                     /locus_tag="AC162G_13955"
     CDS             5169..5894
                     /locus_tag="AC162G_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076956.1"
                     /translation="MIIQPSPDTSIPDTPERHQDGVALMASMPVIVSNDTHAVGAGSP
                     GASRLLVLVPDLDVDEVTLAQCVWSLAEPRSLPVLYLGISSDPFRESPVRRRLATLAA
                     ITRDNRIQVDTRYLPAHNWPEAIQVTCRPGDLIVSPPERALSLQSNGYRSGRKALSAA
                     VGAPVHVLPDPLFDTRPDRRRRLKRFVFWPVSLAILAGFFWLQVQIDQATSGSVQIIL
                     LSISVLAEGGLLLLWHTLTSEEP"
     gene            5891..>6100
                     /locus_tag="AC162G_13960"
     CDS             5891..>6100
                     /locus_tag="AC162G_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076957.1"
                     /translation="MTDNNRLTSHLTPSSAPDSAADATSHLQQEYDAWQALFAAQPTL
                     VQRFLEAQARALADAIPQPHPVSQMR"
BASE COUNT         1106 a         1889 c         1841 g         1264 t
ORIGIN      
        1 gcgcctcagt tcttctgtgt cgggcatcaa ctgatcggag aaatagtccg gcccgatcag
       61 gctgttgccg cacttgatgt tcgcgtcgag gttgggcagc gcgcgctccg ggaacaacgc
      121 caactgccgg cccagcgtct cgtcgctctc gccctccagc accttgagca gcaggctcaa
      181 cttcgtcact tcggccgcct gctggtcgat gtccacgccg aagatgtgcg cggtgaggat
      241 acgcttcttc tcggcggtgg tgagacgcca tgacccgtca ggagcctggt agatcggcac
      301 actacggctt atcacagatc gcttatcgcc ggtcgccata ggcgatacgc tatttgcgat
      361 atgccactgc aggtagtagt cgagcaggta ttcgtacgcg ccgagcagga acgagccgct
      421 gccgcaggcc atatccagca cgcagaagcc ttcgagctgc ttcggcgctt taccttcgac
      481 gagcttgccg acggtgttct tgacgatgta ctcgacgatg taggccgggg tgtagtacac
      541 gccgccggcc ttcttcactt ccggcttctc ttcgactttg gcgtgatgcg acggcgtcag
      601 gcggatgact ttgccgagga actgctcgta cacgcttccc agaatctcgg cgggcaggac
      661 gctgaactcg tagggtgact gcggatagta caggcccgcc aggatcggct tgagaacgcc
      721 gtcgtccacc ttgagcttca gagcgagcgc gtcgcctttg gacgagaagt cgaacagacc
      781 ggcattgtac ttcgcgtcgg cggcgcggca caggtcgatc agcccggcgt agacactccc
      841 ctcacccgtc gacgcctttc cgacgggaga ggctgccgaa ggcgtggggg tgagggccag
      901 caacttcccg tacggctcga tgccgcgatc ctcggccatg cgcaggaaga tgaggcggtc
      961 gatggtgcgc tgcacggcgt cgttcagctc gtcgatggtc aggcgcgggt tgcgcagcgc
     1021 gatgttgcgc gccagcacgt cgcgccagtg ttcgatctct ttcaggaact cggcatcgac
     1081 ctcgcccgtg ccgcgcttgc ccttggccgt ctgcgcgtac gcatcgaacg agccagagcg
     1141 caccgcgccg tacgagaaca cgtcccacag ctcgcgccag cggtcggggt attcctcgaa
     1201 tgtaaggtag ttgatgcgac cgacgctcac cttgtctttg tccgagggcc gcgcccggca
     1261 gtcgtacacc gctagctctt cgaagtcggt gaggatcgac agcgggagtt tcgcgctcca
     1321 ggcgtagcgc cggagctgat aggcatggtc gggattcgtc ttgatcgact cggcaggctt
     1381 cttggcttcg gcgaagaagg ccggatcgcg cccgatgcga aaggcgtagt cgggcgcttt
     1441 cttgcccatt tccgtctcca gcgagtcttc gaggacgacc ggctggtatt gcggcgcggc
     1501 acgctcggcg ttgtacacat cccagtcgag cgcgatgaac agtgggtcga tcaggttttg
     1561 gcgagcgtgg gcttctttgt agccgggggc gtggaaggcg gtacggttgg tctggtagtg
     1621 cttgaccagt cgggcgattt catcgaggga ttggtcgaac gtcttcgcca tcgtcacccc
     1681 tgaagctctg agaggagtca gtcatgcacg tggaagggcg tgccggtcaa cacattgctc
     1741 agttgtgaac tgagcgagtt gcggcggctg ctcatgcccg atacagccgc taccgcgaat
     1801 acggttgcgc cgtcttcaac gcactcaaag cggaatagtg cttctcgttg aacgtcgcca
     1861 gtgtgatacc cagcccaatc gccgtctcgg cgatgagtga atccaacaag cccaggttat
     1921 ggctcaaatg ataggccgtg aagtcagcca atgcccgagc acagtcggtc tgagttggcc
     1981 agtagagtgt gtaggggtgg aggagcgcct cgacgcgctg ctgctcgtct attgtgcgac
     2041 agccttgtag cagttccatc actaccagac cgggcaggcc cagctcctcg gttagagatg
     2101 cttgaagcca ggccacggcc ggtgggtgtt tccgcaggac atcgatcatt acatcggtgt
     2161 caagcaggat catcaccggc cctctcgcga cgttcggcct gtcgacgaag ttgacgcgca
     2221 aactcggcgc tatctgcaat gtcggttcga tcttcccaaa ggccaaccag accggagtcc
     2281 agcagattgg ccgccgtcat aacgcgctgt ggcgccgggg catccaccaa cacgatcact
     2341 tcggcagacg cgcccggtgt caattcgggc gaacgaatct caaccaggcc acccggctgg
     2401 acaatggttt ttgtgcggac tgccagtgtc atcatcatac ctcctgcaat tcagtatagc
     2461 atgtcttggc gctcggtcaa aggcgaggcg atcttaagcg cagcgcctat ttcctacatc
     2521 aattcgcagg ctgaagcctg cactccaatc gccctacacc ctctacttgc cggtggtcaa
     2581 gcgcaacttc ccgtgaacac cgttataatt gtgctggagc actcgatatg cgccgcccag
     2641 gattagcttc gcctccttct ctcgcgcagt acgccttggc ggttttgtcc accgtcaccg
     2701 caacggtcgt gctgttgatc ttgcgggaca gcctgagcac gccgatcgtc gcgctgcttt
     2761 atctcctgcc ggttgtcttc agcacggcga cctgggggtt cggtccgggc gtcgtcgctg
     2821 cgttatgcgc tttcttgtcc ttcaatttcc tcttcattgt accgtactac acgtttgccg
     2881 ttcaccaggc gcacgacgtt ttggcgttgg tcgttttcct gatcgtcgcc gtcgtcatca
     2941 gccggttgct ggggcgcgcc cgagccggcg tgacggctgc gaccgcgcgc gagcaagaga
     3001 cagccagctt gtacgagctg agcatggcgc tggccgggtt gcaggacgag caggcgattg
     3061 cccgcaccat cgccgaacat gtgctggaga cctttcaggc cgagcacgtc gagctgctca
     3121 tcaaggggaa agtcggccac ttccgcgtct ctctgcccga gccgccagct ccgctatcgg
     3181 accagccgat gtgtatgact ccgcttgaga cagcgcgtgg tcgtcaagga gaaattcgtc
     3241 tctggcgcgg tgagccgccg attgcgccgg tcgaagagcg cctgttacgc accttcgcca
     3301 gccagggcgc actggcgctc gaacgcgcac gcctggccca ggcagagacg cgggccaaag
     3361 tgctggagga aagcgaccgg ctgaaatccg cgctcctgtc gtccgtctca cacgagctgc
     3421 gatccccgct ggcggcgatc aaggccggca tcaccagcct gcgcagccgt gaggtgcact
     3481 gggattcacc ggccggcgcc gatctgctcg ccgtcgtgga agaagaagtc gatcacctca
     3541 accggctggt cggcaacttg ctggatatgt cacgcatcga gtcaggcgcg ctcaaaccga
     3601 agcgccaatg gaacgtcctg gtcgacatct tcggcagcgc gctggagcgt atgcaccgcg
     3661 ccgccgaagc gtatcggatc gaaatcgatc tgcgtgacga tctgccactt gtgccggtgg
     3721 atgctgcgca gttagagcag gtcttcacca atctgatcag caatagcctc aagtatgcgc
     3781 cgccgggttc cgtcatctac atcggcgcct gcccgcagga cgaccagacg ctgctcgtcg
     3841 agatgcgcaa tcaaggcccg ccgatcgacg aagagcattt ggagcgcatc ttcgacaagt
     3901 tctatcgtgt gaccgccgcc gatcgggtga ccggcaccgg gctagggctg tcgatttgca
     3961 aagggattgt tgaagcgcac gggggacgca tctgggccga gaaccgaccg gatggcctgg
     4021 ccttcaaatt cacgctgccg ttgacgtggg atggcgcgca gccgcctgtg ctgccgccca
     4081 ccgtgttgag cgagtctgaa tcatcatgag cacacagccg cacatcctcg tcatcgacga
     4141 cgaaccgcaa atcctccgcg ccatgcgcac gatcctcacc gagaagcagt ttcgcgtgac
     4201 gacggccagt cggggcgaag aaggcctggc gctggccgcc gccaatccgc ccgatgtcgt
     4261 catcctcgac ctcggtctac cggatgtcga cggtatcgaa gtctgcgcgc ggctgcgcga
     4321 gtggacgcac atccccatca tcgtgctatc ggtgcgcgac agggaacaag acaaggttgc
     4381 ggccctggat aagggcgcgg acgattacct caccaagccg ttcgcggtcc aagagctttt
     4441 ggcgcgcgtt cgcgttgcgt tgcggcatct cacgcaggcc aaagggagtc caaagaccgt
     4501 tgtcacggct ggcccgatag cgatcgatct gactcgtcac gtcgtgaccc gcgagggtgt
     4561 cgaagtgaag ttgaccgcca ccgagtttaa cctgctggcg tacctggcca cgaatgccgg
     4621 gcggcttctg acgcatgaga gcattttgac acacgtctgg gggccggaag ccgccggtcg
     4681 cgccgagtat ctgcgtgtgt tcatcggcca gttgcgcaag aagctggaag ccgatccgcg
     4741 acggccccgc atgatcctca acgagccggg catcggctat cgttttgttg ccgacgacta
     4801 attcgacaat atcgcattta ggctcaagcc gccgtcctcg gcgcggcgag ccgaagtgtt
     4861 ttgtcgccga cgattaaatc gggtatcacg agtcagcata cagagaacgc cccgatctgg
     4921 ggcgttcttt atttgtccct tacacctccg cttaaatcct ttgcttcaac tttattccgt
     4981 cgatactcct ttatttgtcc cttacgcatt tggtcgaaac ttttgcccgg cccttatttc
     5041 tttttgatac agtctcagca gagcgggctc ccacgcccgc ggatcggcgg catgggagac
     5101 gcctctgcta gcaattctgg atctactgag tctagaggca cacaaccgaa tcgagggcga
     5161 ggcaagagat gatcattcaa ccatcacctg atacttccat acctgacaca cctgaacgac
     5221 atcaggatgg tgttgcattg atggcttcga tgccggtcat cgtgtcgaac gacacgcatg
     5281 ctgttggtgc gggatcgcca ggcgccagtc gcttgctggt gctggtgccg gatctcgacg
     5341 tcgacgaagt caccttagct cagtgcgtgt ggtcgctggc cgagccgcgc agcctgccgg
     5401 tgctctacct cgggatttct tcggatccct tccgagagtc gccggtcagg cgacgtctgg
     5461 ccacgttggc cgccatcaca cgtgataatc gaatacaggt ggatacccgg tatctccctg
     5521 cacacaactg gccggaggcg attcaagtca catgccggcc cggcgattta atcgtctctc
     5581 ctccggaacg agctctctcc ctccagtcga acggctatcg atctgggcgc aaggctctct
     5641 cggcagcggt aggtgcgccg gtgcatgtct tgccggatcc cctcttcgac acgcgcccgg
     5701 atcgccgccg gcgtttgaaa cgctttgttt tctggccggt cagcctggcc atcctggccg
     5761 gcttcttctg gcttcaggtg caaatcgatc aagccacaag tggttcggtc cagatcatct
     5821 tgctcagcat atccgtcctc gccgagggcg gcctgcttct gctctggcat accctgacga
     5881 gtgaggaacc atgaccgaca acaatcgatt gacttctcat ttgactccat catccgcgcc
     5941 ggacagcgcg gccgatgcga ccagccatct tcagcaggaa tacgatgcat ggcaggcgct
     6001 cttcgcagcg cagccgacgc tcgtccagcg ttttctggaa gcgcaggcac gcgcactggc
     6061 cgatgcgatc ccgcagcccc acccggtttc gcagatgcgc
//
LOCUS       DBNHXB010000345        16949 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1770787, whole genome shotgun sequence.
ACCESSION   DBNHXB010000345 DBNHXB010000000
VERSION     DBNHXB010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 16949)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16949)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16949
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1770787"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="AC162G_13965"
     CDS             <1..275
                     /locus_tag="AC162G_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HIR5076958.1"
                     /translation="YSIPISSTKSMIGHAMGAAGALEAIGMTMTVVTQHVHPTINYRE
                     PDPACDLDYVPNEARDARVRVAISNSFGLGGHNACLVLGQYRNGRT"
     gene            293..991
                     /locus_tag="AC162G_13970"
     CDS             293..991
                     /locus_tag="AC162G_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HIR5076959.1"
                     /translation="MKIVQNDKYIKTRGRIGRYAFFGGFGLLVTGLLISLNVNRADLF
                     PISFACLILGILLSQFGGMYMRRFDRGELPHLTLAKALKGFDDRYTLVNYAAPASHVL
                     LTADAVYVLVPKAQAGRISFKNGRWKNPTGIRRIFTWMSEEGLGNPTREAHAEAARLQ
                     RFLARQMPDTPVEVQPIVVFLHPNAELDISDSPVPAVHVKKLKDLLRTRPKGGLSRDM
                     RAALGRLLEPAPAS"
     gene            988..1752
                     /locus_tag="AC162G_13975"
     CDS             988..1752
                     /locus_tag="AC162G_13975"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5076960.1"
                     /translation="MNPEEYEVMYRVEDAHWWYRGMETITRAMLDRAIGQNGNLDILD
                     AGCGTGAVMGYLADYGRVTGFDFAAEALHFCRMRNRDRLSRASVLTLPYADASFDLVT
                     SFDVLCERSVADDWLALSEFARVLRDGGHVLLRLPAYDWLHGKHDEAVHIRHRYTAPE
                     ISSKLRQAGLVPVRVSYANMLLFPLAVLKRASERVLSDRQPGSDLTIGTGRMNAPLRW
                     VLSLEAPFVARTKLPFGLTVVALGQRKAADCLRHNG"
     gene            1795..2577
                     /locus_tag="AC162G_13980"
     CDS             1795..2577
                     /locus_tag="AC162G_13980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5076961.1"
                     /translation="MQQPVKRLSSVTAFFPCYNDAGTIPTMIIRTVQTLHDVADDYEV
                     VVINDGSADDSGRVLDEMARRYDCLRVFHRAQPSGYGGVLRAVFAAATKDWIFYTDGD
                     AQYDARELALLVEAVTDGVDMVNGYKIKRHDPIHRVAIGLAYQYFVKLAFGLVIRDVD
                     CDFRLMRRSIFDRVTLESTTGTITFEMVKKIQDAGFCIVEVPVHHWYRQYGQSQFFNF
                     ARVARTLMAMVGWWWRLVVKREHLRGAQEAAPAQASPREPIP"
     gene            2574..3566
                     /locus_tag="AC162G_13985"
     CDS             2574..3566
                     /locus_tag="AC162G_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5076962.1"
                     /translation="MTLESFFAGKRVLITGGLGFIGSTLARRLVDLEAQVILVDSLIP
                     EYGGNLFNVSGYEDRLRVNIADVRDEFSMDYLVRGQDVLFNLAGQTSHLDSMTDPYTD
                     LEINARSQLSILEACRKHNPTIAIVYASTRQIYGKPDYVPVDEKHPLHPTDVNGVNKM
                     AGEWYHILYHNVYGLRVTSLRLTNTYGPRMRVKDARQTFLGIWLRRIVDGEPIEVWGD
                     GAQRRDFNYVDDVVEAMLMCAFDARADGRIYNLGSRESISLADLARLLIDINGGGEYR
                     LVPFPPDRKRIDIGDYVADYTLIRRELGWSPRTGLREGLTRSLEYFRMHRRHYW"
     gene            3614..5161
                     /locus_tag="AC162G_13990"
     CDS             3614..5161
                     /locus_tag="AC162G_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076963.1"
                     /translation="MHKKTSSRWGAITALLVGLAFPVALTVFQGAVAIGQYQEPVPLK
                     PPFVFHEADPAIGASSFTPLPPTASRLLTETFGVGFDVSFGVVGTTTKWYIFTDTNSV
                     NFYWNRVSPSVSGAYVDTAWVACGTCDGTPGTSLDPDTDDYPANMGTWMIYGPVNLGD
                     YYDAELTFNYRLDADPAVDGSGSGDLFAFGVSDDGTHFTGELHSGSSSFTEWMTPTFK
                     LTDYAGKSSVYLGFYFHSNGDANTGKGVLIDNVSLRAAPYSRSYLPHVARDFAVATPT
                     PTVTPTPTPAPYLYNYTWSVGGENDPDFVAWGQSYTKVEGGTTTYEQGLTGGNPGAGM
                     YLYNTRLFLVTMAGPNVSVSGNYEISAQFNVYKSKDNARYGIIFGADTNAFSRSGSEP
                     RFNANANYYAFALQFPSPPNGNRPADFQLELCNTGDGTNCNKLVDRTPIPSGANADGV
                     WDTLKVRRQGNQITIFVNNVQLATVSDGTLTGTREFGVYIRSANVNGTGTPLEVDWDN
                     YTVTQLP"
     gene            5211..6311
                     /locus_tag="AC162G_13995"
     CDS             5211..6311
                     /locus_tag="AC162G_13995"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR5076964.1"
                     /translation="MNIPPANPQAQYAAHRSEIDAAIACVLDAGHYILGEETRAFERE
                     FAEYVGVEHAIGVGSGTEALHLALRACGIGPGDEVATVSHTAVATVAAIDLAGAAPVL
                     VDIDPHAYTLDPQQLERVITPRTKAVIPVHLYGAPADLESIVSIARRHGLKVIEDCAQ
                     AHGATYRGRRVGSWGDIAAFSFYPTKNLGAIGDGGLVATNDAQLAERARLLREYGWSE
                     RYVSSISGWNSRLDELQAAILRVKLRYLDADNARRRELAAIYDEVLPPGVHRPAAPDG
                     CVHVYHLYVIRHPRRGPLAAYLRDKGIGTGIHYPVPIHLQPAYRGRLAGAGALPETER
                     AAREVLSLPIYPELTTDQARQVAEAIRAFGAW"
     gene            complement(6558..7610)
                     /locus_tag="AC162G_14000"
     CDS             complement(6558..7610)
                     /locus_tag="AC162G_14000"
                     /inference="COORDINATES: protein motif:HMM:NF024813.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR5076965.1"
                     /translation="MPRIIDVVEFVDETENEIVHRIPERGSGDFRFGSNVIVRESQVA
                     IFFRDGKALDVFEPGKHVISTANIPLLIDLLKLGTSGKTPFTAEAYFVSMREFIDKGW
                     GTPEPITMRDADLGMVRLRANGTYNFEVSDPSLFVNKIVGTQGIYETPQVENLLRGLI
                     ISRLTDLLGEMKKSLLDLPAMYDEIGAATKAKAEDDFAARGLRLKTLYVRSISPTEDT
                     AKAIDERAQMGAIGDMAKYIQFQAARGIRDAAQSPSGGVAGAGVGLGAGLGLGQMMAG
                     AMAQSLQAQPQQPQAQQAAAAPAPDVMTPAEAAAYLKVTEEEIKQMIDSGEIKAKKVG
                     KSFRIAKKTLDDFLAS"
     gene            complement(7621..8142)
                     /locus_tag="AC162G_14005"
     CDS             complement(7621..8142)
                     /locus_tag="AC162G_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076966.1"
                     /translation="MGDLMGKVESERGALERLGGKIPGFRGYLAREKRRDADKLLRDA
                     VARRFEAQLRRLPDLQMQLVSAGRIEHVDDVERAVIRLNTFVDRVKTTPRGYAGFFDA
                     VKINEDELEQLYHWDTQLLGEADQIALSIDALAAAIGGTGDTGAAVRDLVASSAAINE
                     LYSKREQALLASV"
     gene            8282..10132
                     /locus_tag="AC162G_14010"
     CDS             8282..10132
                     /locus_tag="AC162G_14010"
                     /inference="COORDINATES: protein motif:HMM:NF053443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5076967.1"
                     /translation="MRSVFERFRRPRPGTQAGEPPQAAAQVVVLTRRQLRLLLAFVAA
                     NVVLLVVLAVLALQPLAAPAVLTIVLTPTPMALPPRQTPTPTAAPATPTPTPDPFGGG
                     GAIGFVLRRNGNSDVYALNIDRNGLIRLTSHPADDRDPAFSPDGAQLAFASHRDGNWD
                     IYRMALESGVTTRLTFSNTYEAAPAWSPDGEWIVFESYREGNFDLYMMRAEDGSRVTR
                     LTSDPAPDVSPAWSPDGKSIVFASYREGNKDIFLLPLDSEQGEEDLANLTNSPDRDED
                     NPAWSSDGLKLAYTSGRPGDQLIYVNTVDPAFRSLQEAEVGLFGQGSHPAWSPDDSAI
                     AFTYHRGSSDVLIAANLGGWGLAREAFGGREFISHPSWSSQPLSEDAVARTVDAAPLA
                     APPLYTEVVSPTASGQTPYVFVRLDDVGPSGHLLSDAVDDSFRALRARVQQETGSDYL
                     AVLGSSWRPMNHTPREGQSRRSYHVAGRAIDIDQTPYNNGGDRIVFVREDIGSVTYWR
                     VYIRTRQQDGTQGEPLREAPWELDAGAETGGRPMARIPAGYYVDFTTLAADYGWERVR
                     AIWRWRSFYPDVEWWHFQKTEGLPWWDAMKQVFRERDVIVSYGPYPGRDN"
     gene            10412..11128
                     /locus_tag="AC162G_14015"
     CDS             10412..11128
                     /locus_tag="AC162G_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076968.1"
                     /translation="MRKMHWIAPVLVVAALLFSACQASQPGAGAPGSQSTGRLPDALS
                     AGLDSLNSYRAAMTMTFDGSRDGQPLREELVWTEEVVRQPAALRVTYSSEADRGDLLE
                     IVSTGGQTYTLLEDECLITQVEGDPKTLIGFDPSTFFGSLSAQSDLGVETIDGIPARH
                     YRADTSGLGFASGFTDAHADMWVAEAGGYVVKYVFEGKGSSADLFGRGDTTPGAVRIE
                     YQVSDVNQAITIAAPESCGS"
     gene            complement(11235..11924)
                     /locus_tag="AC162G_14020"
     CDS             complement(11235..11924)
                     /locus_tag="AC162G_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076969.1"
                     /translation="MLVGQAEMKPALILSVYSKALLFVALLVALAGYFGPWVWHPSVA
                     LRYSADDLAEFVKLMPVVRSGQVSITRELFYLPIWLASIGLALWLGRIARSRWMRWLA
                     GLPVVYASMWPMPTYPFILDAYRSPEFSLSFWVSVLAAALCVIALAFGSRLPDRATAS
                     TWIAIGSTGASVAPLHFVRLQPALDVLHGWTMSIGWGIVAVVIGFLTVAVAGAWQWRQ
                     AGVRHSVAREG"
     gene            complement(11985..12452)
                     /locus_tag="AC162G_14025"
     CDS             complement(11985..12452)
                     /locus_tag="AC162G_14025"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR5076970.1"
                     /translation="MDGKRTSESTVVLSHFMQPEHANAMGNVHGGWLMKLMDEAGGLC
                     ATRHARRPAVTVVVDSLTFVSPVHVGDLVIMEARLTYTGRTSMEVEVHVEAENILTGE
                     RTYTNSAYIIYVALDEHNRPAEVPPLILETDEERERFERARQRQAERLARRAT"
     gene            complement(12525..12770)
                     /locus_tag="AC162G_14030"
     CDS             complement(12525..12770)
                     /locus_tag="AC162G_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR5076971.1"
                     /translation="MTETFDRIKGIIVELLGVDASKVTAEARFREDLEADSLDLVELI
                     MAFEEEFGGEISDEDAQKITTVGQAVEYIETRMPAAK"
     gene            12948..13967
                     /gene="fni"
                     /locus_tag="AC162G_14035"
     CDS             12948..13967
                     /gene="fni"
                     /locus_tag="AC162G_14035"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HIR5076972.1"
                     /translation="MTVEQRKADHIRINLEEDVGFERLTVGFERYRFVHQALPELDLA
                     EIDLATTFLGKPLKAPLLISCMTGGTDQARQINRNLAAAAQQFGIAMGLGSQRAGLVH
                     PDKVDTFKVRDLAPDILLLANLGAVQLNYGYGVDECRRAVDMIQADALVLHLNPLQEA
                     VQPEGDVNFAGLAKKIETVCTQMEVPVIAKEVGWGISERSARLLVNAGVSAIDVAGSG
                     GTSWSQVEMHRAPSDLHRRVAAAFRDWGIPTSESIQMVRRVAPELPLIASGGLRSGID
                     VAKSIALGANIGGMAGPFLKAAAVSVDAACETAEETIRVLRIAMFSAGAGDIDALRQT
                     PLVRM"
     gene            14058..15065
                     /locus_tag="AC162G_14040"
     CDS             14058..15065
                     /locus_tag="AC162G_14040"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR5076973.1"
                     /translation="MPIEHYFARWLPAVEDEMRAIVAAPDLHAFAGLYGMIYYHLGWA
                     DTGFNSASVPAGKRIRPMLCLLACEGAGGDPEAVLPAAAALELLHNFSLIHDDIEDGS
                     DLRRGRPTLWKVWGVPKAINAGDTLYTLAHSAFHRLGARGVPIDRQVEAHLRFCQTCA
                     RLTHGQHLDLDFESRNRVTVDEYIAMIEGKTAALLAGSVAIGAIIAGSIHDSRYAEFG
                     LKLGLAFQIQDDLLGIWGDPAVTGKSAASDIASRKKSLPVLYGLANSGELRELYAAPE
                     IDVEKAVELLELAGAKEYAAQIANDYTQHAMAALDAAAPQGEAGEALRELVQSLLRRQ
                     A"
     gene            complement(15098..16849)
                     /locus_tag="AC162G_14045"
     CDS             complement(15098..16849)
                     /locus_tag="AC162G_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076974.1"
                     /translation="MRTSASEWPTANTDHGLLVAFGEFLQQHGLLARLMQVPLPQKTR
                     TFAPQTKLVEFLAGIMSGIAYLSDLNDGPHPLAKDPIAARAWGQPGFAHYSGVSRTLD
                     VCNAETVAAVERAIADFSRPFIDQVVHDLLRRGEPVIYDLDLTGQAVSPTSQTYPGVA
                     FGWMNDGVKLGYQLARVCLSPPDQDRVWLAGFHHPGDTVSAACVQDLIRAAETHTHIR
                     PRRRPELVQQRIAAHQDSLNRTQRLLEQQQARLTQLQQTQRDLIGKVYHAHEVLKTPI
                     SPGKSTRLQGQVAGWRKRLPRLEQQIATATRVLAQHRARLTQQQVGLADLQAWQAQLE
                     ADNRTNPDAPPYCEVRMDAGFVSGENLTWLIEMGYCPNTKAPNDRTTRALRARVTPRT
                     RWVRVGDNAEMVAWGEYVLHGCPYPLTVALERFKVGHRYKYATLIHYRDDGWFPTLPA
                     WFQHYNARQTIEAGNKEMKGPFHVQHLMSRSLAGIRLQVLFTGLAANVVRWCVPWLYR
                     CAATPTAKFTRVLDSPKHLVRMAANTDALVQHTYFGTALQFGPQSPLSGVTLFLRGVP
                     AFQLALGFNQPCEISSG"
BASE COUNT         3070 a         5594 c         5249 g         3036 t
ORIGIN      
        1 cgtacagtat acccatttcg tcgacgaagt cgatgatcgg ccacgccatg ggcgcggcgg
       61 gcgcgttgga ggccatcggc atgaccatga ctgtcgtcac gcagcacgtc cacccgacca
      121 tcaactaccg cgagcctgat cccgcgtgcg acctggacta cgtgccgaat gaggcgcggg
      181 acgctcgcgt gcgtgtggcc atctctaact ccttcggatt gggcggccac aatgcgtgcc
      241 tggtgctcgg acagtatcgc aacggcagaa cctgattgag cagggtgcgt gcgtgaaaat
      301 cgtacagaac gacaaataca tcaaaacgcg cggccgcatc ggccgctatg ccttcttcgg
      361 cggcttcggc ttgctggtga cggggcttct catttcgctc aacgtcaatc gcgcggacct
      421 gtttcccatc tctttcgctt gcctgattct gggcatcctc ttgtcgcaat ttggcggtat
      481 gtacatgcgc cgcttcgacc gcggtgagct gccgcacttg acactggcga aggcgctcaa
      541 gggattcgac gatcgctaca cgctggtgaa ctacgctgcg cccgcgtcgc acgtgctgct
      601 cacggcggac gctgtctacg tgctggtccc caaagcgcag gccggacgca tctccttcaa
      661 gaacgggcgc tggaagaacc cgaccggcat ccgccgcatc ttcacctgga tgagcgaaga
      721 aggtctgggc aacccgacgc gcgaggcgca cgccgaagcg gcgcgcctgc agcgcttcct
      781 ggcgcggcaa atgcccgata cgccggtcga ggttcagccc atcgtcgtct tcctgcaccc
      841 aaacgctgag ctggacattt ccgattcacc tgtgccggcc gtccacgtca agaaactgaa
      901 ggatttgctg cgcacccgac cgaaaggcgg cctgtcgcgc gacatgcgcg ccgcgctggg
      961 acgcctcctc gaacccgctc ctgcgtcatg aatcccgaag aatacgaagt gatgtatcgc
     1021 gtcgaagacg cgcattggtg gtatcgtggc atggagacga tcacgcgcgc catgctcgat
     1081 cgcgccatcg ggcagaacgg caatctggac attctggatg cgggctgcgg caccggcgcg
     1141 gtgatgggat acctggccga ttacggccgg gtcaccggtt tcgacttcgc cgccgaggcg
     1201 ctgcacttct gccggatgcg gaaccgcgac cggctcagcc gcgcgtccgt attgacgctt
     1261 ccgtacgcgg acgcctcgtt cgatctcgtg acgtcgttcg acgtgctgtg cgagcgctcg
     1321 gtcgccgacg actggttagc cctgagcgag ttcgcgcgcg tcctgcgcga tggcggtcac
     1381 gtgctgctcc ggctgcccgc ctacgactgg ctccacggca agcacgacga ggccgtacac
     1441 atccgccatc gctacactgc tcccgagatc tcgagcaagc tgcggcaggc cggtctcgtc
     1501 ccggtgcgcg tgtcgtacgc caacatgctg cttttcccgc tcgcggtgct gaagcgggcg
     1561 tccgaacgtg tcctgtccga ccggcagccc ggctccgacc tcacgatcgg gacaggccgg
     1621 atgaatgcgc cgttaaggtg ggtgctgagc ctcgaagcgc cgttcgtcgc gcgcacgaag
     1681 ctgccgttcg gcctgaccgt cgtcgcgctg ggccagagaa aggcagcgga ttgccttcga
     1741 cacaacggat aaacgaatga acgcgccgcc gactcaatga ttcaagacat caccatgcag
     1801 caacccgtca agagactctc cagcgtcacc gccttctttc cctgctacaa cgacgccggc
     1861 acgatcccga cgatgatcat ccgcaccgtg cagacgctcc acgacgtcgc agacgactac
     1921 gaagtcgtcg tgatcaacga cggctcggcg gacgactcgg gacgcgtcct cgacgagatg
     1981 gcgcggcgct acgactgcct ccgcgtcttc catcgcgctc agcctagcgg gtacggcggc
     2041 gtgctgcgcg ccgtgttcgc cgccgcgacg aaggactgga tcttctacac cgacggcgac
     2101 gcgcagtacg atgcccgcga gctggcgctc ctcgtcgagg ccgtgaccga cggcgtggat
     2161 atggtgaacg gttacaagat caagcgtcac gatccgattc accgcgttgc catcggcctg
     2221 gcgtaccagt atttcgtcaa gctcgccttc gggctggtca tccgcgacgt ggactgcgac
     2281 ttccgcctga tgcggcgcag cattttcgac cgggtaaccc ttgaatcgac gactggcacg
     2341 attacgtttg agatggtaaa aaagatacag gatgcgggat tctgcatcgt cgaggtgccg
     2401 gtgcatcact ggtatcggca gtacggccag tcccagtttt tcaatttcgc gcgcgtggcg
     2461 cgcacgttaa tggccatggt cggctggtgg tggcggctgg tcgtcaagcg cgagcacctg
     2521 cgcggcgcac aagaagccgc gcccgcacag gccagcccgc gcgagccgat cccatgacgc
     2581 tcgagtcctt tttcgcgggc aagcgcgtcc tcatcacggg gggattggga ttcatcggca
     2641 gcacgctcgc gcggcggctg gtcgatctgg aagcacaggt catcctcgtc gattcgctca
     2701 tcccggagta cggcggcaac ctgttcaacg tcagcggcta cgaggaccgg ctgcgcgtca
     2761 acatcgccga cgtgcgcgac gagttcagca tggactacct ggtgcgcggc caggacgtgc
     2821 tgttcaacct ggccggccag accagccatc tggattcgat gaccgacccg tacaccgatc
     2881 tggagatcaa cgcccgcagc cagttgtcga tcctggaggc gtgtcgcaag cacaacccga
     2941 ccatcgccat cgtgtacgcc agcacgcgcc agatttacgg caagcctgat tacgtcccgg
     3001 tcgacgaaaa gcacccgctc cacccgaccg acgtgaacgg cgtcaacaaa atggcgggcg
     3061 agtggtatca cattctgtac cacaacgtct acggcttgcg cgtcaccagc ctgcgcctga
     3121 cgaataccta cgggccgcgc atgcgggtga aagacgcgcg gcagacgttc ctgggcatct
     3181 ggctgcggcg catcgtcgac ggcgagccga tcgaagtgtg gggcgacggt gcgcagcgtc
     3241 gcgacttcaa ctacgtcgac gacgtcgtgg aggccatgct catgtgcgca ttcgacgcac
     3301 gcgccgacgg ccggatttac aacctgggca gccgggaatc catctcgctc gccgacctgg
     3361 cgcggctgct gatcgatatc aacggcggcg gcgagtatcg gctcgttccg ttcccgcccg
     3421 atcgcaagcg catcgacatc ggagattacg tggcggatta cacgctgatt cgccgcgagc
     3481 taggctggtc gccgcgcacc ggcctgcgcg aggggctgac ccgctcgctc gaatatttcc
     3541 ggatgcaccg gcggcattac tggtgaagcc gcacgttcgg ttggtatgac aacatgcaag
     3601 aagggagact atcatgcaca agaaaacaag ttctcgatgg ggagcgatta ccgccttgct
     3661 ggtcggtttg gcgttccccg ttgcgctcac tgtattccag ggcgccgtcg ctatcggcca
     3721 gtatcaggaa ccggttccgc tgaagccgcc gttcgtattc catgaagccg acccggcgat
     3781 aggggcaagc agcttcactc cgctcccgcc gacggcatcg cgcttgctga ccgagacgtt
     3841 cggcgtcggc ttcgacgtgt cgttcggcgt agtcggcacc acgacgaagt ggtacatctt
     3901 caccgacacg aatagcgtga acttctactg gaatcgcgtg tccccatcag tctccggcgc
     3961 gtatgtggac acagcgtggg tcgcctgcgg cacgtgcgac ggcacgccgg gcacgagcct
     4021 cgatccagac accgatgact atccggccaa catgggcacg tggatgatct acggcccggt
     4081 caatctgggc gactactacg acgccgagct gacgttcaac tatcggctcg acgccgatcc
     4141 ggcggtcgac ggcagcgggt cgggcgacct tttcgcattc ggcgtttccg acgacggcac
     4201 ccacttcacg ggtgagctac attccggatc gtccagtttc acagagtgga tgacgccgac
     4261 gttcaagctg accgactacg ctggcaagtc gagcgtgtac ctcggcttct acttccacag
     4321 caacggcgac gccaacaccg gcaagggcgt gttgatcgac aacgtcagcc tgcgcgccgc
     4381 gccgtacagc cgaagctacc tgccgcacgt cgccagagac ttcgccgtcg ccaccccgac
     4441 cccgacggtc accccgaccc cgacgccggc gccctatctg tacaactaca cgtggtcggt
     4501 cggcggcgag aatgaccccg actttgtcgc ctggggccag agttacacaa aggtagaagg
     4561 cggcacgacc acctacgagc aagggttgac cggcggaaac cccggcgcgg gcatgtacct
     4621 gtacaacaca cggctattcc tggtcacaat ggctggaccg aatgtgagcg tatccggcaa
     4681 ctacgagatc agtgcccagt tcaatgtgta caagagcaaa gacaatgccc gctacggcat
     4741 catcttcggg gctgacacca atgcgttttc gcgcagcggg tcggaaccca ggttcaatgc
     4801 caatgccaac tactacgcgt tcgctctgca attcccgagt ccgccgaacg gcaaccggcc
     4861 ggccgacttc caattggagc tgtgcaacac cggagacggc accaactgca acaagctggt
     4921 tgaccgcacg cccatcccgt ccggcgcgaa cgccgacggc gtgtgggaca ccctgaaggt
     4981 tcggcgacag gggaatcaga tcacgatctt tgtcaacaac gtccagctcg ccactgtcag
     5041 cgacggcacc ttgaccggca cgcgcgagtt tggagtgtac atcaggtccg cgaatgtcaa
     5101 cggcaccggc accccgcttg aggtcgactg ggacaactac accgtgacgc agttgccgta
     5161 acgatccagg tgaccggcac ctgaggggtg ccggtcacct tttgcaagcc atgaatatcc
     5221 cccctgccaa ccctcaggct cagtacgccg cgcatcgttc cgagatcgac gcagccatcg
     5281 cctgcgtgct cgacgccgga cattacatcc tgggtgaaga gacgcgcgcc ttcgagcgcg
     5341 agttcgccga atacgtcggc gtcgagcacg cgatcggcgt cgggtcaggc accgaagcgc
     5401 tgcacctggc gctcagggcg tgcggcatcg ggccgggtga cgaagtcgcc accgtctcac
     5461 acaccgccgt cgccaccgtc gcggcgatcg atctggccgg cgctgcgccg gtcctggtcg
     5521 acatcgatcc tcacgcgtac acgctcgatc cgcaacagct cgaacgggtg attacgccgc
     5581 gcacgaaggc ggtcatcccg gttcatctgt acggcgcgcc ggccgatctc gagtcgatcg
     5641 tctccatcgc caggcgtcac ggcctgaagg tcatcgaaga ctgcgcccag gcgcacggcg
     5701 cgacgtatcg cggacggcgc gtcggatcgt ggggcgacat cgccgccttc tcgttctatc
     5761 cgacgaagaa cctcggcgcc atcggcgacg gcggcctggt cgcgacgaac gacgcgcagc
     5821 tcgccgaacg cgcccgcctg ttgcgcgagt acggctggtc ggagcgctac gtcagctcga
     5881 tctccggctg gaactcgcgg ctcgacgagc tgcaggcggc aatcttgcgc gtcaagctgc
     5941 gctacctcga cgccgacaac gcgcgccggc gcgagcttgc cgcgatctac gacgaggtgc
     6001 tgccgccggg cgtgcacagg ccggccgcgc cggacggctg cgtgcatgtc taccacttgt
     6061 acgtcattcg ccacccgagg cgcggcccac tcgccgcata cctgcgcgac aaaggcatcg
     6121 gcacgggcat tcactatccc gtgccgatcc atctacagcc ggcgtatcga gggcgcctcg
     6181 ctggcgccgg ggccttgccg gaaaccgaac gcgccgcgcg ggaagtcctc tcgctgccga
     6241 tataccccga actcactaca gaccaggcgc gccaggtggc cgaagccatt cgcgcgttcg
     6301 gcgcatggta actactcagg cgtcactgcg agtgccacac atggcacagt aaatcatccg
     6361 gcacggtaga cactcgcgtg ccgcacgatc ggcgccgagc atgtcacgcc ggagggaatg
     6421 acgaggcatc tcgacatcag catcccgcac tcgccgatcg gaggagagat tcctcactcc
     6481 gttcggaatg acacttttga agtttgaagt ttggagttgg gagttgggag tttgagtcag
     6541 acgagagatt cctcccttta cgacgccagg aagtcgtcca gcgtcttctt ggcgattcgg
     6601 aaggattttc cgaccttctt cgccttgatc tcgccggaat cgatcatctg tttgatctct
     6661 tcttcggtga ccttcaagta ggccgccgcc tcggcgggcg tcatcacgtc gggcgccggc
     6721 gcggcagccg cctgctgcgc ttgaggctgt tgcggctgag cctgaagcga ttgcgccatc
     6781 gcgccggcca tcatctggcc gaggcccagc cccgcgccca gccccacgcc ggcgccggcc
     6841 acgccgccgg atggactctg cgctgcgtcg cgaatgccgc gtgccgcctg aaactggatg
     6901 tactttgcca tgtcgccgat ggcgcccatc tgcgcgcgct cgtcgatcgc cttcgccgtg
     6961 tcctccgtgg gcgagatcga gcgaacgtac agcgtcttca gtctcaggcc gcgcgccgcg
     7021 aaatcgtcct ccgccttggc cttcgtcgcc gcgccaatct cgtcgtacat cgccggcagg
     7081 tcgagcagcg actttttcat ctcgcccagc aggtcggtca accggctgat gatcaggccg
     7141 cgcaggagat tctcgacctg cggcgtctcg taaatgccct gcgtgccgac gatcttgttg
     7201 acgaacagcg acggatcgga cacctcgaag ttgtaggtgc cgttggcgcg caggcgcacc
     7261 atgcccaggt cggcgtcgcg catcgtgatc ggctccggcg tgccccaacc cttgtcgatg
     7321 aattcgcgca tgctgacgaa atacgcttcg gcggtaaacg gcgtcttgcc gctggtgccg
     7381 agcttcagga gatcgatcag gagcggaatg ttggccgtgc tgatgacgtg cttgcctggt
     7441 tcgaacacgt ccagcgcctt cccgtcgcgg aagaagatcg cgacctggct ctcgcgcacg
     7501 atgacgtttg agccgaagcg gaagtcgccc gacccgcgct ccggtatacg atggacgatt
     7561 tcattttcgg tttcgtcgac aaattcgacg acgtcgatga tgcgaggcat agttttctcc
     7621 ttacacagat gcgagcagcg cctgctcgcg cttcgaatat agctcgttga tcgccgcgct
     7681 ggacgcgacc aggtcgcgca cggccgcgcc ggtgtcaccc gtcccgccga tcgccgccgc
     7741 aagcgcatcg atcgacagcg cgatctgatc cgcttcgccg agcaactgcg tatcccaatg
     7801 atacaattgc tccagctcgt cctcgttgat cttgacggcg tcgaaaaagc cggcgtagcc
     7861 gcgcggcgtc gtcttgacgc gatcgacgaa tgtgttcagc cggatcacgg cccgctcgac
     7921 gtcgtcgacg tgctcgattc tgccggcgga cacaagctgc atctgcaggt cgggcagccg
     7981 gcgcaactgc gcctcgaagc gtcgcgcgac ggcgtcgcgc agcaatttgt cggcgtcgcg
     8041 tcgcttctcg cgcgcgaggt acccccggaa accgggaatc ttgcctccga ggcgctccag
     8101 cgccccacgc tcgctttcca ccttgcccat gagatcgccc attcatccac tccttgcccg
     8161 gtcgtctggt acacacagta taacacaact cccccgcgcc cacagagcgc aaaacgcact
     8221 gttatgctcc tgactcacgt actccttgac acgccgatac agcccgtgta tagtcgctgc
     8281 catgcgctcg gtgtttgagc gattccgccg acctcggcct ggcacacagg ccggcgagcc
     8341 gccgcaggct gcggcgcaag tggtcgtttt aacacgtcgc cagttgcgcc tgctgctggc
     8401 cttcgtcgcg gccaacgtcg tcctgctcgt cgtcctcgcc gtgctggccc tgcagccgct
     8461 cgccgcgccg gccgtgctga ccattgtcct cacgccgacg cccatggcgc tgccgcctcg
     8521 ccaaacgcct acgccgacgg ctgcgccggc taccccgacc cccacgcccg atcccttcgg
     8581 cggcggcggc gcgatcgggt tcgtactgcg gcgcaacggc aactccgacg tctacgctct
     8641 caacatcgat cgcaacgggc tgatccgcct caccagccat cccgccgacg accgcgaccc
     8701 ggccttctcc cccgacggcg cgcagctcgc cttcgcctcg catcgggacg ggaactggga
     8761 catctatcgt atggcgcttg aatcgggcgt cacgacccgc ttgactttca gcaacacgta
     8821 cgaagctgcg ccggcctggt cacccgacgg cgaatggatc gtgttcgaat cgtatcgcga
     8881 gggcaacttc gacctgtaca tgatgcgcgc cgaggacggc agccgcgtga cccgcctgac
     8941 gagcgatccc gcgccggacg taagtccggc ctggtcacct gatggcaaaa gcatcgtctt
     9001 cgcctcttac cgcgagggca acaaggacat cttcctcctg ccgttggatt cggaacaggg
     9061 cgaggaagac ctggctaacc tgaccaacag cccggaccgc gacgaagaca acccggcgtg
     9121 gtcgagcgac gggctgaagc tggcatacac gtccggccgg ccgggcgatc aactcatcta
     9181 cgtcaacacg gtcgatccgg cgttccgcag cttgcaggaa gccgaggtcg gcctgttcgg
     9241 gcagggcagc catccggcct ggtcgcccga cgacagcgcc attgcgttta cctaccaccg
     9301 cggatcgagc gacgttctga tcgcggcgaa cctgggcggc tggggcctgg cccgcgaggc
     9361 gttcggcggc cgcgagttca tcagccatcc atcctggtcg tcacagccgc tctccgagga
     9421 tgccgtcgcc cgcaccgtcg acgccgcgcc gctcgccgca ccgccgctct acaccgaggt
     9481 cgtcagcccg actgcatccg gccagacccc atacgtgttc gtccgtctcg acgacgtcgg
     9541 ccccagcggc catctcttga gcgatgccgt tgacgactct ttccgcgcgc ttcgcgcacg
     9601 cgtccagcaa gagaccggga gcgactacct ggccgtgctc ggttcgtcgt ggcgcccgat
     9661 gaaccacacg ccgcgcgagg ggcagagccg ccgttcgtat cacgtcgctg gacgggctat
     9721 cgacatcgac cagacgccgt acaacaacgg cggtgaccgc atcgtcttcg tccgtgagga
     9781 tatcggctcc gtcacctact ggcgcgtata catccggaca agacagcaag acggcacaca
     9841 aggcgagccg ctgcgcgagg cgccctggga gctcgacgcc ggcgcggaga cgggcggcag
     9901 accgatggca aggattcccg ccggctacta cgtcgatttc acgacgctcg ccgccgacta
     9961 cggttgggag cgcgtgcggg ctatctggcg ctggcgctcg ttctatcccg atgtcgagtg
    10021 gtggcacttt cagaagaccg aaggcttgcc gtggtgggac gcgatgaagc aagtgttccg
    10081 ggagagagac gtcatcgtct cctacggtcc ctaccccgga cgcgacaact gaccctcgcg
    10141 cctgcccctc tacgatgggc ctatccgcaa accccggggc gacctgtatt cctctggtgc
    10201 acgaaccaca cacatccagc cgcttgccgc cggatttggc acgcccttcg ggcagcgacg
    10261 ggagcggcga gagtgccgat ttattccctt gcacgttctt tgagccagaa ttaaggacac
    10321 gagtcatctt tcgccgctgc gggcttgtgt gttacgatac gagcacattt ccgctcaggc
    10381 gtcacgccga gctaagtgag gaacgagaaa gatgagaaag atgcactgga ttgcccccgt
    10441 tctggtcgtc gccgcgctgc tcttctcagc ctgccaggcg tcccagccgg gagcgggcgc
    10501 tcccgggtcg cagtcgaccg gccgcttgcc ggacgcactg tctgctggcc tggacagtct
    10561 gaacagctac cgcgccgcga tgacgatgac gttcgacgga agccgagacg gccagccgct
    10621 ccgcgaagag ctggtgtgga cggaggaagt cgtccggcag cccgccgctt tgcgcgtcac
    10681 ctactcgtcg gaggcggatc ggggagactt gttggaaatc gtcagcacgg gcggccagac
    10741 ttacaccctg cttgaggacg aatgtctcat cacgcaggtg gaaggcgatc cgaagaccct
    10801 catcggcttc gatccatcga ctttcttcgg cagcctctcc gctcagagcg atctgggcgt
    10861 cgagacgatc gacggcatcc cggcccgcca ctatcgagcg gatacgtccg gtctggggtt
    10921 tgcctccggg ttcaccgatg cgcacgccga catgtgggtg gccgaggcgg gcggctacgt
    10981 cgtgaagtac gtcttcgaag gcaagggcag cagtgcggat ctcttcggcc gaggcgatac
    11041 gacgcccgga gccgttcgca tcgagtacca ggtcagcgac gtgaaccagg cgataaccat
    11101 cgccgcaccc gaaagctgcg gcagctgagt ctggacgttc cgttcatgcc ggacgtggcc
    11161 gacgtcagct ccgtcgaaga cttgctcaac tgatctacct cgtcaaccat cgccgacgtg
    11221 gtcgccttct gctcttatcc ttcgcgcgcg accgaatgtc tcacgccggc ctgccgccac
    11281 tgccacgcac ctgccacggc gacggtcaaa aatccgatca ccacggcgac gatgccccag
    11341 ccgatgctca tcgtccaacc gtgcagtacg tcgagcgccg gttgcaggcg aacgaagtgc
    11401 agcggtgcga ccgacgcacc ggtcgatccg atcgcgatcc acgtgctcgc cgtagcgcga
    11461 tcggggagcc gcgatccgaa ggccagcgcg atcacacaca aagctgccgc gaggacgctg
    11521 acccagaaag acaggctgaa ctcgggtgat cgatatgcat ccaggatgaa gggatacgtc
    11581 ggcatcggcc acattgaggc gtagacaacc ggcaggccgg cgagccatcg catccacctg
    11641 ctgcgcgcga ttcggcccag ccacagcgcg aggccaatcg acgccagcca gatcggcaga
    11701 tagaacagtt cgcgggtgat cgacacctgg ccggagcgga ccacgggcat gagcttcacg
    11761 aactcggcca gatcgtccgc cgagtagcga agtgcgacgg agggatgcca gacccacggc
    11821 ccgaaataac cggccagcgc gacgagcagc gcgacgaaca atagagcctt ggaatacacg
    11881 gacagaatca gcgccggctt catttctgcc tggcccacca gcacgagaaa cgttcaccct
    11941 cgtaatacac ttcaaaggct ttctgcgtct cgcacgtgtt caggctacgt cgcgcgtcgg
    12001 gccagccgct cggcttgccg ctgccgggcg cgctcgaagc gctcgcgctc ttcgtcggtc
    12061 tccaggatca gcggcggcac ttcggccggg cggttgtgtt cgtcaagcgc cacgtagata
    12121 atgtacgcgc tgttcgtgta agtgcgctcg cccgtcagga tgttctccgc ctcgacgtgc
    12181 acttcgactt ccattgacgt gcggccggtg tacgtgagcc gcgcttccat gattacgaga
    12241 tcgccgacgt gcactggcga cacaaaggtg agcgagtcga cgactacggt caccgccggc
    12301 cggcgcgcgt ggcgggtggc gcacaggccg ccggcttcgt ccatcagctt catcaaccac
    12361 ccgccgtgca cgttccccat cgcattcgcg tgttcgggtt gcatgaagtg tgacaggacg
    12421 accgtcgact cggaggtacg ctttccgtcc atcgacctta aaccaaaaca aacgggccgg
    12481 cgatccgaat gagatcgcgg cccgccggaa tggcccgtac gcagctattt ggcggcaggc
    12541 atgcgcgttt cgatgtactc gaccgcctgg ccgaccgtcg tgattttctg agcgtcttcg
    12601 tctgaaatct caccgccgaa ttcctcctcg aacgccataa tcagctcgac cagatcgagt
    12661 gaatccgcct caagatcctc gcggaagcgc gcctcggctg tgactttgga cgcgtcgacg
    12721 cccaacaatt ccacgatgat gcctttgatg cgatcgaatg tttccgtcat gtgcccctcc
    12781 taggtgtgcg cgtgggaatg gccgtatttt agccggttga ccctgattgt caagcgaaaa
    12841 cgggcgcagt gcttgacaat ggtttgagac gtgttagact tgcgctgccg taccattcaa
    12901 accccgcgcc tccttgaaag ttgcgcgaat tccctcaaag agagcgcatg accgtcgagc
    12961 agcgtaaagc tgaccacatc cgcatcaatc tcgaagaaga cgtgggcttc gagcgcctga
    13021 ccgtcggttt cgagcggtac cgattcgtcc accaggcgct gcccgagctt gatctggccg
    13081 aaatcgacct ggcgactacg ttcctgggca agccgcttaa agcgccgctg ctgatctcgt
    13141 gcatgaccgg tggcacggat caggcgcgcc agatcaatcg caacctggct gccgccgcac
    13201 agcaattcgg catcgccatg ggtctcggct cacagcgcgc gggcctggtg caccccgaca
    13261 aggtggatac gttcaaggtg cgcgacctgg cgcccgacat cctgctgctc gccaacttgg
    13321 gcgccgtgca gctcaactac ggttacggcg tcgacgagtg ccgccgcgcc gtcgacatga
    13381 ttcaagccga cgcgctcgtg ctgcacctca acccgctgca ggaggccgtc cagcctgaag
    13441 gcgatgtgaa cttcgccggc ttggcaaaaa agatcgagac cgtgtgcaca cagatggaag
    13501 tgccggtcat cgccaaggaa gtcggctggg gcatttccga gcgctccgcg cgcctgctcg
    13561 tcaacgcggg cgtgtcggcg atcgacgtcg ccgggtcggg cggcacatcg tggagccagg
    13621 tggaaatgca ccgcgcgccg agcgacctgc accggcgcgt cgccgccgca ttccgtgact
    13681 ggggcatccc taccagcgaa tcgatccaga tggtgcgccg cgtcgcaccc gagctgccgt
    13741 tgatcgccag cggtgggctg cgctcgggca tcgacgtcgc caagtcaatt gcgctcggcg
    13801 cgaatatcgg cggcatggcg gggccgttcc tgaaggccgc cgccgtctcg gtcgatgccg
    13861 cgtgcgaaac cgctgaggag accattcgcg tgctgcgcat tgcgatgttc agcgcaggcg
    13921 cgggagacat cgacgcgctt cggcaaacac cgctcgttcg catgtgatgg cgcgcgttgc
    13981 gtctgcccct gggcgaccgt ttgggtgacc gcaagggtgc atccttgggc gactgcgagg
    14041 gtcgccccta cgaccttatg cctatcgaac actactttgc acgctggctg cccgccgttg
    14101 aagatgagat gcgcgcgatc gtagccgcgc ccgatttgca cgccttcgcc ggcctgtacg
    14161 gcatgatcta ctatcatctt ggctgggccg acacgggctt caattcggcg tcggttccgg
    14221 ccggcaagcg catccggccg atgttgtgcc tgctggcgtg cgaaggcgca ggcggcgatc
    14281 ccgaagccgt cctgcccgct gcggcagcgc tcgagctgct gcacaatttc tcgctcatcc
    14341 acgacgacat cgaagatggt tccgacctgc gccgaggccg ccccacgctg tggaaggtgt
    14401 ggggcgtccc gaaggccatc aatgccggcg acacgctgta caccctggcg cactcggctt
    14461 ttcatcgcct gggcgctcgg ggcgtgccga tcgatcgcca ggtcgaagcg catctgcgct
    14521 tctgccagac ctgcgcgcgc ctcacgcacg gacagcacct cgatctggac ttcgagtcgc
    14581 gcaatagagt gaccgtcgac gagtacatcg caatgatcga aggcaagacg gcggcattgc
    14641 tggccggctc ggtcgccatc ggcgcgatca tcgcaggcag catccacgac agccgttacg
    14701 ccgagttcgg cctgaagctg ggcctggcgt ttcagatcca ggatgacctg ctgggcatct
    14761 ggggcgatcc ggccgtcacc ggcaaatcgg cggccagcga catcgccagc cggaagaaat
    14821 cgctgccggt gctgtatggg ttggcgaaca gcggggaact gagggaactg tacgccgcgc
    14881 cggagattga tgttgaaaag gcggtggaac tactggagct ggccggcgca aaggagtatg
    14941 cagcacagat tgccaacgat tatacacagc acgcgatggc cgcgctcgac gcggcggccc
    15001 cgcaaggcga ggcgggcgag gcgctgcgcg aactggtgca gagcttgtta aggaggcaag
    15061 cctagcgtaa gttttgcgca accagagggc aaatcgttca tccagatgaa atttcgcatg
    15121 gctggttgaa gccgagcgcc agttgaaagg ccggaacgcc tcgcaaaaac agcgtgacgc
    15181 cagacaaggg actctgtggc ccaaactgca aggcggttcc gaaataggtg tgctggacca
    15241 gcgcatcggt attcgccgcc atgcgtacca gatgcttggg gctgtccagc acgcgcgtga
    15301 acttggcggt gggtgtcgcg gcgcagcggt acaaccaagg cacacaccag cgcacgacgt
    15361 tggcggccag accggtgaag agcacttgca gacgaatgcc ggccagggaa cgactcatca
    15421 agtgttggac gtgaaagggg cctttcatct ctttgttgcc agcttcaatc gtctggcgtg
    15481 cattgtagtg ttggaaccaa gccggtaaag tggggaacca gccgtcatcg cggtaatgaa
    15541 tcagcgtggc gtacttgtac cgatgtccga ctttgaagcg ttccaaggcg accgtcagcg
    15601 gatagggaca cccgtgcagg acgtactcgc cccaggcgac catctcggca ttgtccccca
    15661 ctcgcaccca gcgggtgcgc ggcgtgacgc gagcgcgcag ggcgcgcgtg gtccggtcgt
    15721 tgggagcttt ggtgttggga caatagccca tctcgatcag ccaggtcagg ttctcccccg
    15781 acacgaagcc ggcatccatg cgcacttcac agtacggtgg cgcgtcgggg ttggtgcggt
    15841 tgtcggcttc cagctgcgcc tgccaagcct gcaggtcagc cagtccgacc tgttgctgag
    15901 tcagccgcgc ccggtgctgc gccaggacgc gcgtcgccgt ggcgatctgc tgttccagac
    15961 gcggcaggcg cttgcgccag cccgccacct gcccctgcaa acgggtcgat tttccaggcg
    16021 aaatcggcgt tttcaggact tcgtgtgcat gatacacctt tccgatcagg tcacgctgtg
    16081 tctgctgcaa ctgagtcagc cgcgcctgtt gctgctccag caagcgttgc gtccggttga
    16141 ggctgtcctg atgcgcggcg atgcgttgct gcaccagttc ggggcgtcgc cgcggtcgaa
    16201 tgtgcgtgtg cgtctcggca gcgcggatca ggtcttgcac acacgcagcg gagacggtgt
    16261 cgccgggatg atgaaaaccg gccagccaca ctcggtcttg gtccggcggc gacagacaca
    16321 cgcgtgccag ttgatagccg agtttcacgc cgtcgttcat ccagccaaac gcgacaccgg
    16381 gataggtctg actggtcgga ctgaccgcct gaccggtcaa gtccagatcg tagatcaccg
    16441 gctcgcctcg gcgcagcagg tcgtgcacca cctgatcgat gaacgggcgg ctgaagtcgg
    16501 cgatggcccg ttccacggcg gccaccgtct ccgcgttgca gacatccagc gtgcggctga
    16561 cgccactgta gtgcgcgaag cccggctgac cccaggcccg cgcggcgata gggtctttcg
    16621 ccagcgggtg cgggccatca ttcagatccg acagatacgc gatgccgctc atgatcccgg
    16681 ccaggaactc caccagcttg gtctgcggcg caaaggtgcg cgtcttctgt ggaagcggta
    16741 cctgcatcag gcgcgccagc agtccatgtt gttgcagaaa ctcgccgaag gcgaccaaca
    16801 ggccgtggtc ggtgttcgcc gtcggccact ccgaagcact tgttctcaca ggcaatccct
    16861 ccgcacgaga gggtacctgc ctcggcgtgg ccgtgcaaaa ttacatccgt ttcaacgatt
    16921 cgggcgctga ttgcgcaaaa gttacgcta
//
LOCUS       DBNHXB010000346         9812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_932881, whole genome shotgun sequence.
ACCESSION   DBNHXB010000346 DBNHXB010000000
VERSION     DBNHXB010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_932881"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="AC162G_14050"
     CDS             <1..1096
                     /locus_tag="AC162G_14050"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR5076975.1"
                     /translation="AEASRYVFFEPGKPQIVGGLRPENALPRGGSTTPHVHEPGVRLN
                     LIDAVKRTLEHELAINPRALIFGEDVGVKGGVHGATTELQLRYGADRVFDTSLSEEGI
                     VGRAIGLAYAGLMPVPEIQFRKYADPATERIHDLGWLRWCTAGRFAAPMVIRIPCGTG
                     RRTGDPWHSVSSEVAFAHAVGLRVAFPSNAEDAVGLLRSALRGDDPTIFLEHRALLDT
                     VQARRPYPGDDYVIPYGVAKVVQEGDELTVVSWGETLHRCLEAARDFEGRVEIVDLRT
                     IVPWDREAVLRSVRKTSKALIAHEDTWTAGFGAEIAATIAQEAFMDLDGPIVRLAMAD
                     HPIPYNPRLLEAVIPTVGKIRAEIQRLLAC"
     gene            complement(1199..2170)
                     /locus_tag="AC162G_14055"
     CDS             complement(1199..2170)
                     /locus_tag="AC162G_14055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5076976.1"
                     /translation="MHYRTFGRTGWQVGDVGYGMWGMGGWSGSDDEESMRSLHRAVEL
                     GCNFFDTAWAYGEGRSERLLGQLVRAHPDKKLYTATKIPPKNSRWPARPEYTLDETFP
                     PDHIRRYTESSLRNLGLDSVDLIQFHVWEDGWADDESWQRAADDLKREGLIRAIGISI
                     NRWEPENAIRALRTGLIDAAQVIYNIFDQAPEDRLFPVCRELNVGVIARVPFDEGTLT
                     GTLTLDSKWPEGDWRNAYFVPENLKASVERAEALRPVIPSGMSMPEMALRFILSNPDV
                     STVIPGMRRIRHVESNIACSDAGGLAPELIQQLRAHRWDRKPTWWSQ"
     gene            complement(2179..3021)
                     /locus_tag="AC162G_14060"
     CDS             complement(2179..3021)
                     /locus_tag="AC162G_14060"
                     /inference="COORDINATES: protein motif:HMM:NF025615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HIR5076977.1"
                     /translation="MATTASTKTRSHWLKKVYALPAEHGAWALWLGPLAVGIGVAGWN
                     GLPTLLLVLAQSGVFLTRQPMVILVKALSGRRSRDDAAPALVWLTVYTALAAIFGAAL
                     IVLGYPKLLWAIVPIAPMLIWQVWLIRQRAERQMSIELAGSGMLALAAPTAHYVAAGV
                     VDATAIMLWVLCWLQSAAAIVYVYLRLAQRRLSEMPPMPERWRMGRRALLYAGANFAL
                     SVGLAIGGLVPPLTPIAFGAMLAQAVGGARNPCVGVKPQRIGFAQVAATAIFSALLIV
                     AYRV"
     gene            complement(3026..4378)
                     /locus_tag="AC162G_14065"
     CDS             complement(3026..4378)
                     /locus_tag="AC162G_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCD family MFS transporter"
                     /protein_id="HIR5076978.1"
                     /translation="MNVTTAPRFSFLRSLKIGSFNIGSAMVDILTASVWNRILIADLG
                     ASATPVSILLALRYLLAPVSVWIGTRSDARLVLGLRRIPYIWLGRLMMLAGIVLLPIT
                     TLRLSSDIDDPAGWLLALAIFVFYGLGTAVSGGLYLALIHDSAPPAKRGVAISIAQTL
                     LIAGFAASPILYARLLPSYTPERFQALALLTGVVAGGVWFLSVLGEERRTAPTGGNAG
                     PAKTQLNLRATLHSMWSDRRTRLFFSFLALGAMSSFAQDAVLEPFGGDVFGLSADATT
                     RFSAYFGGGVLAAMIVTSIVTRRKRPEEQTGPATLGLSVMIASLALLAAAGLSLTAWL
                     ITPALIGFGLGFGVYTVGGVSLLMAMTTEASAGAYLGLWTMTQLVSRGVGIGLGGVVR
                     DVGLALTGSLAVAYSSVFVLEAIGLAVCIAILSRLDVVGFARDHRVETTPSPMVALAD
                     "
     gene            complement(4648..5253)
                     /locus_tag="AC162G_14070"
     CDS             complement(4648..5253)
                     /locus_tag="AC162G_14070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR5076979.1"
                     /translation="MLARLFVQWSFLMGIEMDIFEAIYNRQSIGKVKPDPIPRDLIEK
                     LLVAAVQAPNHHKVRPWRFVVLTGAARERLANAMVDALRKRKPDADDDSLARERRKPL
                     RSPVLIAVGVDKPNIPKVIEIENVCAVAAAVQNMLLAAHALGLGAMWRTGDAAYDPHV
                     KTFLGFEPQQHLIGFVYVGYPESEPIPPSRPSYQDRTMWLE"
     gene            complement(5280..6119)
                     /locus_tag="AC162G_14075"
     CDS             complement(5280..6119)
                     /locus_tag="AC162G_14075"
                     /inference="COORDINATES: protein motif:HMM:NF015213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HIR5076980.1"
                     /translation="MLPSFLLAFREGVEAALIVGIVLGVLRRMNRTQYAPYLWLGVAA
                     AIGVSVLGAITLNLIGAEFEGAAEQAFEGVTMLLAAGVLTWMIFWMQRQGRRIQAGLE
                     ADVKMALSRGQAWALFSLAFFAVVREGIELALFLTAANFSAVEAGQAPFLGWLGGALG
                     LIGAVIVGWLVFDAAIKLNLRRFFQLTSVLLIVFAAGLVGTAVHEFNELGWIPPVLEH
                     IYDLNAVIPESSTFGQFLKTLLGYNGNPSLTETIAYAGYFVAIIFLVRWMDRRVATSP
                     ASV"
     gene            complement(6261..6404)
                     /locus_tag="AC162G_14080"
     CDS             complement(6261..6404)
                     /locus_tag="AC162G_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076981.1"
                     /translation="MTLSSYVLLKPPPFDGASHGNRRLSSTAPGLPLKPPFRRRNSDR
                     QRL"
     gene            6429..7112
                     /locus_tag="AC162G_14085"
     CDS             6429..7112
                     /locus_tag="AC162G_14085"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR5076982.1"
                     /translation="MKDKARVTETIENYLESIYNMRDEGKQVIAARLAERLGVKPPTV
                     SQTLHRMERDGLIKIESNEIWLTKLGTEVAEAAIRRHRLVERFMTDFLGIGWAEAHRE
                     AEKLQHAMSDLIEQKMAEALGHPQSCPHGNPIPGVRSTKLPPDALTLDTAHAGQRVAV
                     LRITEEGERDPRLLDYLQRSNLVPGARATVEEVAPWSGVITLILNGQKVPLGLKAART
                     IWVQPVDRR"
     gene            7224..7580
                     /locus_tag="AC162G_14090"
     CDS             7224..7580
                     /locus_tag="AC162G_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR5076983.1"
                     /translation="MLDQYVPIAILFVVSAALAVVLILIGTLFGPRRSNPRKSQPYES
                     GVVPFGQAQRRFPVHFYLIAVLFILFDVEIIFLFPWAVMFKALGFFGLIEMVIFIAIL
                     VVGYVYIWKKGALEWE"
     gene            7571..8080
                     /locus_tag="AC162G_14095"
     CDS             7571..8080
                     /locus_tag="AC162G_14095"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR5076984.1"
                     /translation="MGVENKLGNMGIVTTTLEKAVNWARSNAMWPLGFGLACCAIEFI
                     STQASSYDISRFGMELVRGSPRQSDLIIVSGRLTQKMAPVLRRLYDQMPEPKYIIAMG
                     DCAACGGVFNNYAIVQGIDEIVPVDVYVAGCPPRPEALIDGIIKLQEKVMRSRPFHEA
                     QPKAGAVHV"
     gene            8073..8585
                     /locus_tag="AC162G_14100"
     CDS             8073..8585
                     /locus_tag="AC162G_14100"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR5076985.1"
                     /translation="MSDPIVETLKARFGDAITGVVEFRGETTIHVRPDAIADVCAFLH
                     DDEAASYDFLSDVSAVDVWPDEPRFQVNYHLYSMERNTRLRLKVRVRDGQSLPSVTGV
                     WPAANWFERETYDLFGVKFDNHPDLRRILLPDDYLGYPLRRDAPLVVEEVEFTYNYDE
                     VEARKPYAKE"
     gene            8644..>9812
                     /gene="nuoD"
                     /locus_tag="AC162G_14105"
     CDS             8644..>9812
                     /gene="nuoD"
                     /locus_tag="AC162G_14105"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HIR5076986.1"
                     /translation="MTFDELKGLVSDRAFSGETLLLNMGPQHPSTHGVLRLLLELDGE
                     RVVSCVPDIGYLHTGIEKTCESKTYTKVIPLTDRMDYLSPMSNNLGYVMAVEKLMGVD
                     VPLRAQYIRVILCELQRIASHLVWLGTHAIDLAAMSMLLYCFREREMILDIFEMVSGQ
                     RMMASYFRIGGLWRDVPEGFEDAVRQFMSLFPSHIDEYEALLRNNPLWLSRTKGIGAI
                     SGQDALAMGMTGPSLRGSGVNWDIRKAEPYSGYDHFEFDVPLGENGDVYDRYWCRMRE
                     FRQSLRILDQALKKLPKGPTITGDRKVAPPPKEELAWSMESLIHHFKLWTEGFSPPPG
                     EVYVSVESPRGEFGSYINSDGSPKPRRVHFRTPSFANLSALPGMAKGVLVADVVAL"
BASE COUNT         1847 a         3137 c         3131 g         1697 t
ORIGIN      
        1 ggctgaggca tcccggtacg tcttcttcga gccgggtaag ccccagatcg tcggcggcct
       61 gcgccccgaa aatgcgctcc cgcgcggcgg atcgaccacg ccgcacgtcc acgagccggg
      121 cgtgcgcctc aacctgatcg acgcggtcaa gcgcacgctc gagcacgagc tagcgatcaa
      181 cccgcgcgcc ctgatcttcg gcgaggatgt cggcgtgaag ggaggcgtgc acggcgcgac
      241 gaccgagctg caattgcgct acggcgcaga tcgcgtgttc gatacgtcgc tctccgagga
      301 gggcatcgtc gggcgcgcga tcggcttggc ttacgccggg ctgatgcccg tgcccgagat
      361 tcagttccgc aaatacgccg acccggcgac cgagcgcatt cacgacctgg gctggctgcg
      421 ctggtgcacg gcgggccgct tcgccgcgcc gatggtcatc cgcatcccgt gcggcacggg
      481 gcgcaggacc ggcgatccgt ggcacagcgt gagcagcgag gtggcgttcg cccacgccgt
      541 cggcctgcgc gtcgcgttcc cgtcgaacgc cgaagacgcg gtgggcctgc tgcgctcagc
      601 gctgcgcggc gacgatccca cgatctttct ggagcatcgc gctctgctcg acacggtcca
      661 ggcgcgccgc ccgtatccgg gcgacgatta cgtgattccc tatggtgtgg ctaaggtcgt
      721 gcaagagggc gacgagttga cggtcgtctc gtggggcgag acgctgcatc gctgcctgga
      781 ggcggcgcgg gatttcgagg gccgggtcga gatcgtcgac ctgcgcacga tcgtgccgtg
      841 ggatagggaa gcggtgctgc gctccgtgcg caagacgagc aaggcgctga tcgcccacga
      901 ggatacctgg acggccggct tcggcgcgga gatcgccgcg acgatcgcgc aggaggcgtt
      961 catggacctt gacgggccga tcgtccgcct ggcgatggcc gatcatccga tcccctacaa
     1021 cccgcgcctg ctggaggcag tgattccgac ggtggggaag attcgggccg agatacagag
     1081 gttgctggcg tgttgaggtt ggaggttgga agttagaggt tggaggttgg atgacggcac
     1141 gcggttaatc ggctgcgatt agcgatatgc gatacgcgat atgcgatacg ctatccgctc
     1201 actgcgacca ccatgtcggc ttgcggtccc agcggtgcgc gcgcaattgt tgaatgagtt
     1261 ccggcgccag cccgcccgcg tcgctgcacg cgatgttgct ttcgacgtgc ctgatcctgc
     1321 gcatgccggg gatgacggtg ctcacgtcgg gattcgatag gataaagcgc agcgccatct
     1381 ccggcatgct catgcccgaa gggatcacgg gacgcagcgc ctcggctcgc tcgacgctgg
     1441 ccttcaagtt ctccggcacg aagtaggcgt tgcgccagtc gccttcgggc cacttgctgt
     1501 cgagcgtcag cgtcccggtc agcgtgccct cgtcgaacgg cacccgcgcg atgacgccga
     1561 cgttcagctc gcggcagacc gggaacaggc gatcttcggg tgcctggtcg aagatgttat
     1621 agatgacctg ggcagcgtcg atcagtccag tgcgcagcgc gcggatcgcg ttctccggct
     1681 cccagcgatt gatgctgatc ccgattgccc taattaaccc ctcgcgcttg agatcatctg
     1741 ccgcgcgctg ccagctctcg tcgtccgccc aaccatcctc ccacacgtga aactggatca
     1801 ggtcgacgct gtccagcccg agatttcgca ggctgctctc tgtgtatcgg cgaatgtgat
     1861 cgggtggaaa agtctcatcg agcgtgtact ccggcctggc cggccacctg gagttcttgg
     1921 gcggaatctt ggtggcggtg tacagtttct tatctggatg cgcgcgaacc aactggccga
     1981 gcagcctctc gctgcggccc tcgccgtagg cccacgccgt gtcgaagaag ttgcagccca
     2041 gctcgacggc gcgatgcagc gagcgcatcg actcttcgtc gtccgatccg ctccagccac
     2101 ccatgcccca cataccgtag ccgacgtcgc cgacctgcca gccggttcga ccgaaagtcc
     2161 ggtaatgcat gctgccccct atacgcgata agccacaatc agcaacgccg agaagatcgc
     2221 cgtcgccgcg acctgcgcga aaccgatgcg ctgcggcttc acgccgacgc agggattgcg
     2281 cgcgccgccg acggcctgcg ccagcattgc gccgaacgca atcggcgtca ggggcggcac
     2341 gaggccgccg attgccagcc ccacggacag cgcgaagttc gcccctgcgt acagcagagc
     2401 gcgccggccc atgcgccagc gctcgggcat aggcggcatc tcgctcagcc ggcgctgggc
     2461 gagccggaga tagacgtaca cgatcgccgc cgccgactgc agccaacaca atacccatag
     2521 catgatcgcc gtcgcgtcga ccacgccggc tgcgacgtag tgcgccgtcg gcgcggccaa
     2581 ggccagcatg cctgacccgg ccagctcgat cgacatttgg cgctcggcgc gctggcgaat
     2641 cagccatacc tgccagatca acatgggcgc aatggggacg atcgcccaca gcagtttcgg
     2701 gtagcccagc acgatcagcg ctgcgccgaa gatcgccgcg agcgccgtgt acacggttag
     2761 ccacaccagc gcaggcgctg cgtcgtcgcg cgagcgccgc cccgacagcg ccttcacgag
     2821 aatgaccatc ggctgccgag tgagaaacac gcccgactgc gccagcacca gtaatagcgt
     2881 cggcaggccg ttccacccgg ccacgccgat gccgaccgcc agcggcccga gccagagcgc
     2941 ccacgcgcca tgctcggcgg gcagtgcgta aaccttcttc agccagtgtg accgggtctt
     3001 cgtcgatgcg gttgtcgcca tttcctcaat ctgccaacgc caccatcggt gaaggggttg
     3061 tctcgactcg atggtcgcgc gcgaatccga ccacgtccag ccgcgacagg atcgcgatgc
     3121 acaccgccag cccgatcgct tcgaggacga acacgctgct gtaggcgacg gccagagacc
     3181 cggtgagcgc cagtcccacg tcgcgcacga cgccgccgag gccgatgccc acaccccgcg
     3241 acacgagctg cgtcatcgtc cacagcccca ggtatgcgcc tgcgctcgct tccgtcgtca
     3301 tcgccatcag gaggctgacg ccgcctacgg tgtacacgcc gaagcccagc ccgaacccga
     3361 tcagcgccgg tgtgatcagc caggccgtca ggctcaaccc ggcggcggcg agcagcgcca
     3421 ggctagcgat catcacgctc aggccgagcg tcgccgggcc cgtctgctcc tcgggccgtt
     3481 tgcggcgcgt gacgatcgac gtgacgatca tcgccgccag cacaccgccg ccgaaatagg
     3541 cgctgaagcg cgtcgtcgca tcggcgctca ggccgaacac gtcgccgccg aaaggctcca
     3601 gcacggcatc ctgcgcgaat gaagacatcg cgccgagcgc gagaaacgag aagaacagcc
     3661 gcgtgcgccg atcggaccac atgctgtgca gcgtcgcgcg caggttcaat tgcgttttgg
     3721 ctgggccggc gttgccgcca gtgggcgccg ttcgccgttc ttcgccgaga accgacagga
     3781 accagacacc gcctgcaacc acgcctgtca ggagggccag cgcctggaag cgctcgggag
     3841 tgtacgatgg cagcaaccgc gcgtacagaa tgggcgacgc ggcaaagccc gcaatcagga
     3901 gagtctgggc gatactgatt gcgacgccgc gcttcgcggg cggcgcgctg tcgtgaatca
     3961 gcgcaaggta aagaccgccg gacacggcgg tgccaagccc atagaacacg aagatggcga
     4021 gcgccaagag ccagccggcc ggatcgtcga tgtcgctgga caggcgcagg gtcgtgatcg
     4081 gcagcagcac gatccctgcc agcatcatca aacgccccag ccagatgtag gggatgcgcc
     4141 gcaagccgag caccaggcgc gcgtccgagc gcgttccgat ccagaccgag actggcgcga
     4201 gcaggtagcg cagcgcgagc aggatcgata ccggcgtcgc gctcgcgccg aggtcggcga
     4261 tcaggatgcg gttccacacc gacgccgtga gaatgtcgac catcgccgag ccaatgttga
     4321 aagagccgat tttgagtgag cgcagaaagc tgaagcgagg ggcggttgtg acgttcaaat
     4381 ctcacctatc tgaaatctaa gctaatctaa tgctgagttt cggcaaccgg tgagatttta
     4441 tttgattaga acaggtaagt caagatgtga aagactgctg actcaaactc ccaacttcaa
     4501 aaatgtcatt cccagcgcag cgaggaatct ctcctccggc cggcgagcgc ggcgcgccgg
     4561 ggtagagatg cctcgtcgtt cctcctcggc atgacacgag gggtgctgat cgctcagccg
     4621 tgtcattccg aacgcagtga ggaatctcta ttccagccac atcgtgcggt cttggtaaga
     4681 tgggcgagat ggtgggatcg gctccgattc gggatagccg acgtagacga accctatgag
     4741 gtgctgctgt ggctcgaagc cgaggaaggt tttcacgtgc gggtcgtatg cggcatcgcc
     4801 ggtgcgccac atcgcgccga gtcccagcgc gtgcgcggcg agcagcatgt tctggactgc
     4861 cgccgcgacg gcgcacacgt tctcgatctc gatcactttt gggatattcg gcttgtccac
     4921 gccgaccgcg atcagcacgg gcgagcgcag cggtttcctg cgctccctgg cgagcgaatc
     4981 gtcgtccgcg tccggcttcc tcttgcgcaa ggcatcgacc atcgcgttgg cgagccgctc
     5041 gcgcgccgcg ccggtcagca cgacgaagcg ccacggccgc accttgtgat ggttgggcgc
     5101 ctgcacggcc gccaccagca gtttctcgat caaatctcgc ggaatgggat ccggcttcac
     5161 cttgccgatt gactgccgat tgtagatggc ttcaaagatg tccatctcga tccccatgag
     5221 aaatgaccac tgaacgaaga gcctcgccag cataggcgag gctctcctta cgttcctgtc
     5281 taaacggaag ccgggctggt cgccacgcgg cgatccatcc agcgcaccag gaaaataatc
     5341 gctacgaagt agccggcgta ggctatcgtt tctgtcaacg acgggttgcc attgtagcca
     5401 agcagcgtct tgaggaactg gccgaacgtg gacgattcgg ggatcacggc gttgaggtcg
     5461 taaatgtgct cgaggacggg tggaatccag cccagttcgt tgaactcgtg gacggcagtg
     5521 ccgaccagcc ctgcggcgaa gacgatcaac agcacgctcg tgagctggaa gaagcgccgc
     5581 aggttgagct tgatcgccgc gtcgaacacc aaccagccga cgatgacggc cccgatcagc
     5641 cccagtgcgc cgcccagcca tccgaggaag ggcgcttgcc cggcttcgac ggccgagaaa
     5701 ttggcggcgg tcagaaacag cgccaactcg atgccctccc gcaccacggc gaagaacgct
     5761 aacgagaata gcgcccacgc ctggccgcgc gacagcgcca ttttgacatc ggcctccaaa
     5821 ccggcctgaa tacggcgtcc ctgcctctgc atccagaaaa tcatccacgt caagacgcca
     5881 gcagccaaca gcatggtgac gccttcgaat gcttgttctg cagcgccctc aaactctgcg
     5941 ccgatcaggt tgagtgtgat cgcgccgagc acgctgaccc cgatagcggc agcgacgccc
     6001 agccacagat agggtgcata ctgggtccgg ttcattcgcc tcagcacacc caacacaatg
     6061 ccgacaatca gcgcagcctc gacgccttcc cggaaggcga gcaggaaact gggtagcatg
     6121 gagtcctccc cgaatttagt agattaggat atactaatct cgtgcgcgat gctatacaaa
     6181 cgaatacgaa ttgtcaaggc taactggctg ctaattagca tcctgccggt tatcctaatc
     6241 atgcaagatg cacagcttcg tcataggcgc tggcgatccg aatttcgccg ccggaacggc
     6301 ggcttgagcg gcaagccggg tgcggtcgag gacagtcgcc gattgccgtg gcttgcgccg
     6361 tcgaagggtg gcggcttgag cagcacgtaa gatgacagtg tcaaaccagc tcggcggagg
     6421 atggctcgat gaaagacaaa gccagggtca cggagacgat cgaaaactat ctcgagtcga
     6481 tctacaacat gcgcgacgag ggcaagcagg tgatcgccgc gcggctggcc gagcggctcg
     6541 gcgtgaagcc gccgacggtc agccagacgc tgcaccgcat ggaacgcgac gggctgatca
     6601 agatcgaaag caacgaaatc tggctcacca agctgggcac tgaagtggcc gaggcggcca
     6661 ttcgccgcca ccgcctcgtc gagcggttca tgaccgactt tctgggcatc ggttgggcgg
     6721 aggcgcaccg ggaagccgag aagctgcagc acgcgatgag cgatctgatc gagcagaaga
     6781 tggccgaggc gctgggccac cctcaatcct gcccgcacgg aaacccgatt cccggcgtcc
     6841 ggtcgacgaa gctgccgccc gatgcgctca cgctcgacac ggcgcatgcg ggccagcggg
     6901 tggcggtgct gcgcatcacc gaggaaggcg aacgcgaccc gcgcctgctc gattatcttc
     6961 agcggagcaa cctggtgccc ggcgcgcgcg cgaccgtcga ggaagttgcg ccgtggagcg
     7021 gcgtgatcac cctgatcctt aacggccaaa aagtgccgct tgggctgaaa gcggcgcgca
     7081 cgatctgggt gcagcctgtt gaccgtcgat gatcgaacaa tccaaagttt gttgacactc
     7141 atatgtgctt gtgataaaat caacaacgtt gcggcggctg ttcctagtcg ccgcgtcttc
     7201 taatccttac cctgggagcc cacatgctgg atcagtacgt tcccattgcg atcctctttg
     7261 tggtgtctgc ggctttggcg gtggtgctca ttctcatcgg cacgctcttc ggcccgcgcc
     7321 gcagcaatcc ccgcaaatcc cagccgtatg aatccggcgt cgtgcccttc ggccaggcgc
     7381 agcgccggtt ccccgtgcac ttctacctca tcgccgttct cttcatcctc ttcgacgtcg
     7441 aaatcatttt cttgtttccg tgggccgtga tgttcaaagc gcttggcttc ttcggcctca
     7501 tcgagatggt catctttatc gcgatcctgg tagtgggcta cgtttacatc tggaagaagg
     7561 gggcgctgga atgggagtag agaacaagct ggggaacatg gggatcgtga cgacgacgtt
     7621 ggagaaggcc gtcaactggg cgcgcagcaa cgccatgtgg ccgctgggct tcggcctggc
     7681 ctgctgcgcg atcgagttca tttcgacgca ggcgtcgagc tacgatattt cgcgcttcgg
     7741 catggaactg gtgcgcggca gcccgcgcca gagcgatctc atcatcgtct cgggccgcct
     7801 cacgcagaag atggcgcccg tgctgcgccg gctgtacgat cagatgcccg agccgaagta
     7861 catcatcgcc atgggcgact gcgccgcgtg cggcggcgtt ttcaacaatt acgccatcgt
     7921 gcagggcatc gacgagatcg tcccggtcga cgtgtacgtg gccggctgcc cgccacgtcc
     7981 ggaggcgctg atcgacggga tcatcaagct gcaagagaag gtgatgcgca gccggccgtt
     8041 ccacgaagcg cagccgaaag cgggcgcggt ccatgtctga tccgatagtc gaaacactca
     8101 aagcacgctt cggcgacgcc atcacgggcg tcgtcgaatt tcgcggcgag acgacgatcc
     8161 acgtccggcc ggacgcgatc gccgacgtgt gcgcgttcct gcacgacgac gaagctgcga
     8221 gttatgactt cctatcagac gtgtcggcgg tcgacgtgtg gccggacgag ccgcgctttc
     8281 aggtgaacta tcatctgtac tcgatggagc gcaacacgcg gctgcgcctg aaggtccgcg
     8341 tgcgcgacgg gcagtcactg cccagcgtca ccggagtgtg gccggcggcg aactggttcg
     8401 agcgcgaaac atacgacctg ttcggagtga agttcgacaa ccatcccgac ctgcgccgca
     8461 tcctgctgcc cgacgactat ctcggttatc ctctgcgccg ggacgcgccg ctcgtcgtcg
     8521 aggaagtcga gtttacttat aactatgacg aagtcgaagc gcgcaagccg tacgcaaagg
     8581 aatgatcgag ttccttagag agtttctatg agttcgtcag ttgcccctcc gcgttccatc
     8641 agtatgacct tcgacgagct gaagggcctg gtcagcgatc gcgccttctc cggcgagacg
     8701 ctgctgctga acatgggccc gcagcacccg tcgacgcacg gcgtgctgcg cctgctgctc
     8761 gagctcgacg gcgagcgggt cgtctcgtgc gtgccggaca tcggctacct gcacaccggc
     8821 atcgagaaga cctgcgaatc gaagacctac acgaaggtca ttccgctgac cgatcgcatg
     8881 gactacctgt cgcccatgtc gaacaacctg ggctacgtca tggcggtcga gaaactgatg
     8941 ggcgtcgacg tgccgctgcg cgcgcaatac atccgcgtga tcctgtgcga attgcagcgc
     9001 atcgccagcc acctggtatg gctgggcacg cacgccatcg acctggcggc gatgagcatg
     9061 ctgctgtact gcttccgcga gcgggagatg atcctggaca tctttgagat ggtgtccggg
     9121 cagcgcatga tggcgtccta tttccgcatc ggcggcctgt ggcgcgacgt gccggagggc
     9181 ttcgaggatg cggtgcgcca gttcatgagc ctgtttccat cgcacatcga tgaatacgag
     9241 gcgctgctgc gcaacaaccc actgtggctc agccgcacga agggcatcgg cgcgatctcc
     9301 ggtcaggatg cgctggccat ggggatgacc gggcctagcc tgcgcggcag cggcgtgaac
     9361 tgggacatcc gcaaggcaga gccgtattcc ggttacgatc acttcgagtt cgacgtgccg
     9421 ctgggcgaga acggcgacgt gtacgaccgt tactggtgcc gcatgcggga attccggcag
     9481 tcgctgcgca tcctggatca ggcgctcaag aagctgccga aagggccgac catcaccggc
     9541 gaccgcaagg tcgcgccgcc gcccaaagaa gagctggcgt ggtcgatgga gtctttgatt
     9601 catcacttca agctgtggac ggaagggttc agcccacccc ccggcgaggt gtatgtcagc
     9661 gtcgagtcgc cgcgcggcga gtttggcagt tatatcaaca gcgatggctc gcctaagccg
     9721 cgccgcgtcc acttccgcac gccgtcgttc gccaacctct cggcgctgcc cggcatggcg
     9781 aaaggcgtgc tggtagccga cgtggtggcg ct
//
LOCUS       DBNHXB010000347        14876 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_933351, whole genome shotgun sequence.
ACCESSION   DBNHXB010000347 DBNHXB010000000
VERSION     DBNHXB010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 14876)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14876)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14876
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_933351"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..450
                     /locus_tag="AC162G_14110"
     CDS             178..450
                     /locus_tag="AC162G_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HIR5076987.1"
                     /translation="MEWRIEGFLWLDWVVEKIVEKHGVDPEEVEEAFFNPPYKVRRAQ
                     SGKYLLFGRTEEGRYLFIVFAWEGRQIKPITARDMTDRERRFFERK"
     gene            459..719
                     /locus_tag="AC162G_14115"
     CDS             459..719
                     /locus_tag="AC162G_14115"
                     /inference="COORDINATES: protein motif:HMM:NF023857.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HIR5076988.1"
                     /translation="MTRIPKFANDQEEAEFWATHDATEFLDETEPLDVTFVDARPESA
                     RISLPLDPEVIDNVKAIARERGVDYKTLIGTWVKERLEKELS"
     gene            935..3556
                     /locus_tag="AC162G_14120"
     CDS             935..3556
                     /locus_tag="AC162G_14120"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034335 - DNA negative supercoiling
                     activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR5076989.1"
                     /translation="MLGNIHTIDIEQEMRGAYLDYAMSVIVARALPDARDGLKPVHRR
                     ILYAMHDMGLRPASPYKKSARIVGEVLGKYHPHGDVSVYDAMARMAQDFSMRYMLVDG
                     QGNFGSVDGDPPAAMRYTEARLSRLAEELLADIDKDTVDWLDNFDSTLKEPSVLPSLL
                     PNLLLNGASGIAVGMATNIPPHNLGEVADAIAFLIDRWESIVEDGRASATAAGQPFGH
                     DEEKALLRDLFYAADDGVTIEDLMQFVKGPDFPTGALVLGREGIVNAYAAGKGRIVMR
                     GVANIEEMKGGRYRIAITEIPYQVNKSALIERMAELVRDERISDISDIRDESDRNGLS
                     VVIELKRGSQPKTVLNKLYKYTALQSTFGVQMLALVDGQPRLLSLKRALQLFVEHRQS
                     VITRRSEFDLAKARHRAHVLEGLRIALANLDAVIKTIRESPDADTARARLMERFKLTE
                     IQAQAILDMQLRRLAALERQKIEEEYQQVMQTIAYLEDLLANPRKILALVKEETIALR
                     DKYGDARRTRIAAEATEEMSIEDLVADEEVLIQLTERGYVKRLPVKAFRAQARGGRGV
                     TGIQTREEDAVEYIFATRTLHHVLFFSDKGKVYQLRAYEIPDADRTAKGVPLVNLINL
                     GEGERVTAAVSVPDFDPATYFTMCTRHGRIKRVSAEEFESVRPSGIIAISLDEGDVLG
                     WVKVTRAEQDIIIVTERGQALRYNSSLVRPMGRAAAGVNAIRLGDGDCVTSMEAIVGD
                     GELLVVTANGFGKRTPLEQYPAKGRATGGVRTLANRIDETGIIASARVVWPEDEVTII
                     TAGGIALRTAVANIARSGRSTRGVRVMALLNGDTVASVARIEGGERAVAESSEPIEEV
                     APVVGNNGQEPVAEATK"
     gene            3646..4521
                     /locus_tag="AC162G_14125"
     CDS             3646..4521
                     /locus_tag="AC162G_14125"
                     /inference="COORDINATES: protein motif:HMM:NF017020.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR5076990.1"
                     /translation="MSRFCDGLLARFGDQILRSILYGSFARGEAHAESDVDVMVVVGW
                     EFEKLPGGWYASPYGDPRWEAIIDLSVEATLDCERYVSPLVLSEALFYDTSTEAAREA
                     RREGIELYGPASGVTPAAQYLATPGASAGGTIRSATPRVLKETEPAPDYDTEPADLGN
                     PRLWLALADEKLRASRRLADDDYRNDAVSRAYYGMFYAAKAALLSVGVTVKTHGGVNS
                     EFGRLFVKTGRIAHRYQTMLTHASEDRLRSDYAPKTRPSQENVERIIRDAEVFITKAR
                     ELVEEELSKRGGALP"
     gene            4518..5885
                     /locus_tag="AC162G_14130"
     CDS             4518..5885
                     /locus_tag="AC162G_14130"
                     /inference="COORDINATES: protein motif:HMM:NF016232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5076991.1"
                     /translation="MITSTVLRSGPSQLVEYLPEADVDALAETLVAFANSDGGTALLG
                     VDENGRPNGRVFPDEAETALRAAEARCRPSVQVRWEQVEMTGAAVVAVHVARSTELHS
                     LGDGRVLVRMGAENRPLGGEAIRQLAATKSSGDFESEAVPGATRADLDEAIIAEFVEK
                     REERQRKRLTVGVDEMLVEIGALDAAGHPTTAGILLFGRNPQQFIPQSGLVFVKFIGT
                     EPRGEGGLPGYGRREEIGGPLARIIETAWEAVWEEMHVGAVVKGLQREERTEYPPFAV
                     REALVNAVCHRDYRLKGRRIEIRMFADRLEVISPGGLPGFITVDNIVEEHFSRNPRIV
                     SGLFQWAYIEELGLGIDRMIEEMTQAGHPPPHFKATPYSFTVALSNAQVRRATPAWEK
                     VMNERQARALEYIREHGQITNREYQQLCADVTPETLRLDLSDLVNRGILLRVGAKKGT
                     YYIMK"
     gene            5942..8248
                     /locus_tag="AC162G_14135"
     CDS             5942..8248
                     /locus_tag="AC162G_14135"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR5076992.1"
                     /translation="MPPLLSWVVAGLASAAAYIVLALVLVRRTGFRDSISRVAVLYLG
                     SSALWSVGYAISVWHGLNPSAHQLGVEAAFDLIWIFPPLILILTLQFLERPGAKGVAI
                     IGAMWIGFGVLAGLNVFGLRETLVRFQISETFKDAINPVRIIGWLSFSISALALTVAD
                     YLRTQRPLHRNRIMFWLIALLLIQVGEGLTLMESRRVDPQQIGLVVRLFGAITASYAI
                     LSYHLPNLRSFMRGASAAVLLTLVAGALYLVSFVLFQFVVNSQFGLALTVGLTAGIAL
                     ALAVIQQPVLRIIRSLLDRFLYRQQYDPAQALREYSAAISNILDLDQLVSVAVGIVSE
                     ALEVRRGALILITDLANGNTDVRLVKGMGGLELDRFELDAGSPILDEWRTGRQPLTQY
                     EIDILPRYRSASAVERTYLQALDMEVYVPIRSQSVLVGMFALGRKDSGDPYTPTDFDV
                     LMTLGDQTAVALQNARLVTDLKRANAEITQLNDELTSTNRRLEKLDQAKTDFISIASH
                     ELRTPLTQVRGYADILADSVREGSPSLPQMAQISQGIGRAALRLEEIVTAMLDISQID
                     AQALAITRTPLSIMSVLRFAMDNYKEAVKARSQIVTLEGIDDLPPVQGDFQRLCQAFL
                     NVIGNCIKYTPDGGTISITGRTLAGEPGRDSAAFVEIVIADSGIGIDKADQELIFEKF
                     YRVGPVELHSTGQTKFKGGGPGLGLPIAKGVIQAHGGKIWVESEGQDETRFPGSTFHI
                     LLPAAPASVIAEFRAQSQAEAARFTSAP"
     gene            complement(8232..9248)
                     /locus_tag="AC162G_14140"
     CDS             complement(8232..9248)
                     /locus_tag="AC162G_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR5076993.1"
                     /translation="MSDAYIAIDLGGTRIRAARCRADGTIEARAEQWTHAEDPPDVVF
                     GRIVRTARSVWPDSPPAAIGIGAPGPLDPYTGVLYSVVNIPSLAELPLRDRMRDIFGV
                     PAYVGNDANVAALAEWRYGAARGHADVVYLTISTGIGGGVIIDNKLLLGSKGLGAELG
                     HVSLDYRGRPDKCGNIGCLEALASGPAMRQRAIERIAAGEYSLLRDQARGNLSEITVE
                     MLHEAAERGDRLAASVIHDAAEAIGFGVVSFLHIFNPSIVVIGGGVTNLGDRLFAPIR
                     DVVSRRVMDRRFVVPIVLAELKENVGLLGALALALDPPTQKEADRLSDRRPDDERHDA
                     TARK"
     gene            9396..9812
                     /locus_tag="AC162G_14145"
     CDS             9396..9812
                     /locus_tag="AC162G_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5076994.1"
                     /translation="MNHTYRYPPVLRAPALFVSLLAGGILLGSVIAEIEAADVPDAIL
                     HGAGMVAGIAMLWLGLEFGMRRITPTREDISTRLLRERTIPWREVRDVRDGPFGVLII
                     QPKRGLPIVIWPYLEDFSALLEAVTNFSNSSTPSTS"
     gene            9887..10417
                     /locus_tag="AC162G_14150"
     CDS             9887..10417
                     /locus_tag="AC162G_14150"
                     /inference="COORDINATES: protein motif:HMM:NF017816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing Zn-dependent protease"
                     /protein_id="HIR5076995.1"
                     /translation="MPTSAGVATPHPDLSVFVDTVYYRVAGSTADELRVQMDRSRPGN
                     FDAHTEWYVSWSYPYSEAEGSCTTGPVTISVNITFTLPQWNTPANASEDLVDRWNAYL
                     AALQVHEDGHKGIGIEAANEILQAMNALPAYRFCEELKQAADAAGERVLSEYRQREAI
                     YDQTTNHGATQGARFP"
     gene            10516..10827
                     /locus_tag="AC162G_14155"
     CDS             10516..10827
                     /locus_tag="AC162G_14155"
                     /inference="COORDINATES: protein motif:HMM:NF017922.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase-like
                     domain-containing protein"
                     /protein_id="HIR5076996.1"
                     /translation="MPKTNSVVNDKERHMLIVKFKDGASAELPWDVLRDACPCAECKD
                     LHGTGDPLRLTAMPNKTLTGFEYAGNYAVILIWGDGHRFGIYTWPYLRELAQKLAASL
                     S"
     gene            10837..11319
                     /locus_tag="AC162G_14160"
     CDS             10837..11319
                     /locus_tag="AC162G_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR5076997.1"
                     /translation="MIDPRFLDTLRKMHTRLASTDVNWVVTGSLGFALQGMPVEPHDI
                     DIQSDEKGVYEIERLFSEYVTRPVALSSTETMHSHFGALSIDSLKVEIMGDIQKRLSD
                     GTWEAPVDLNRYKRFVEIEGMRIPVLSLEYEYQAYLKLGRIERAEMLKNWLLDMSNDG
                     "
     gene            11309..13855
                     /gene="mutS"
                     /locus_tag="AC162G_14165"
     CDS             11309..13855
                     /gene="mutS"
                     /locus_tag="AC162G_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR5076998.1"
                     /translation="MTDDLTPIRKQYLALKNQYPDTILFFRLGDFYETFDADAETVAR
                     ELDIVLTSRPVGKGQRVPLAGVPHHAVDRYLAQLIEKGFRVAVAEQMADPSAVTGIVP
                     REVTRVVTPGTILDPEMLDEKRANYLAAWVEHDGRVGLAHCDITTGEFAVAQVDSNAA
                     ALQELLRIAPRECLHPQSQSAPAQASAAIHFTPLPDWKFEAANARQALLDHFDVATLA
                     GFGIDGQSLAIRAAGAICQYLRETQPGALAQLSSLRSYSTGRFMALDPATRRNLELTE
                     TIRERKTQGSLLGVLDATRTAMGGRLLRAHISQPLLDRAELEARLDQVQAFFGDALTR
                     AKARETLKGMPDLERAVGRVVGGSAAPRDMVAIRRALELVPELQESISNLQSPISNLQ
                     IDPCADVLAMLQAAIADEPTTPPGLIRPGYSAELDGILSASRNAKEWVAGLEKTERQR
                     TGIHTLKVGYNKVFGYYIEVTNANRDAVPDNYIRKQTLTNAERYITPELKEYETLILN
                     AGERLVEVENRLFKEICAQVSSRARALRMAAQAIAQIDVASALAEVAALNAYVRPELI
                     DADEIDIVAGRHPVVERTLSETRFVPNDLKLGADTRILIITGPNMSGKSTFLRQAALI
                     ALMAQIGSFVPAEQARVGLVDRIFTRIGAQDEIAAGQSTFMVEMVETANILHHASARS
                     LIVLDEIGRGTSTYDGLSIAWAVVEYVHNHPRLKSKTLFATHYHELIALAETLPHVRN
                     VNVAVAEEGDRVVFLHKIVEGGADRSYGIHVAQLAGLPKPVVHRAEEILQQLEQGDPA
                     HAPVSGVQQLSLFATESPALEELKSIDVDALSPLEALTKLYELKKKASTD"
     gene            13930..14433
                     /locus_tag="AC162G_14170"
     CDS             13930..14433
                     /locus_tag="AC162G_14170"
                     /inference="COORDINATES: protein motif:HMM:NF020471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HIR5076999.1"
                     /translation="MSPTQDVYRELKALTRQRIRLIWEKAQLGGSLSDEDAHLVEAMR
                     EHSEYTDLWGQLDHLSDEQIERDGTNPILHVIIHQTIENQIAGGEPEQTGRTVEALMR
                     QALSRHEASHRVGEVLAGEIWHILKEDRPFDEAGFVRKLRRLAKPGAAHSDHAPRPHR
                     RRPSRKR"
     gene            complement(14567..>14876)
                     /locus_tag="AC162G_14175"
     CDS             complement(14567..>14876)
                     /locus_tag="AC162G_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077000.1"
                     /translation="SAGWVRFGLNQARFGDVEYSDSFRLALLDVARTALDTVANGFLM
                     AGGVAGGIAIGLIAWGYTTPKTRRMVPAVAPVTSAQPPSSPQPAAQDQPSGEPSGTST
                     "
BASE COUNT         2808 a         4543 c         4818 g         2707 t
ORIGIN      
        1 agcgtggcaa actggctgtc acagtgccga tgcacagggg cgatctcccc tcaagcgtgg
       61 ttcgctcaat cctctgaata atcagcagtt acgtggcagt tacgattgcc ttgacatgcc
      121 tgctgcaatg tcataaaata acttcggctc gattccggga cgccgtgagg caaccacatg
      181 gaatggcgca ttgagggttt tctctggctt gattgggtgg tcgagaagat cgtcgagaaa
      241 catggcgtcg atccggaaga agttgaagaa gccttcttca acccgcctta taaagtgcgt
      301 cgggctcaat cgggtaaata tctgctgttc ggccgaaccg aagaaggcag gtacctattc
      361 atcgtattcg cctgggaagg tcgccagata aagcctatca cggcacgcga tatgacggat
      421 cgcgagcgac gcttcttcga acgcaagtga gattctttat gacaaggatt ccgaagtttg
      481 ccaatgatca ggaagaggct gagttctggg ccacgcatga cgcaaccgag tttctggacg
      541 aaacagagcc gctggatgtg acgttcgtgg atgctcgtcc ggagtcggcg cgtatctcct
      601 tgcctctcga tcctgaggtg atcgataatg tgaaggccat agcgcgggag agaggtgtgg
      661 attataagac gctaattggc acgtgggtga aggaacgtct tgagaaggaa ttgagctgat
      721 tctgtgcccg atgagcaggc tttcgagata gcgtaagacg gatgcaacag ttacgatgat
      781 ctgaatgtag aggattgggg cctttctcgt gcgagaaagg cccttttttc ttacgttttt
      841 cctactcttc cggtggcata cgacttagcc ttatgcgcat ggctggggta taattggcat
      901 gtttcctcgc attcttggag gagtttgacc gtcaatgctg ggcaacattc acaccatcga
      961 catcgaacag gaaatgcgtg gggcgtacct ggattacgcg atgagcgtga tcgtcgctcg
     1021 cgcgctgccc gacgcgcgcg atggcctgaa gccggtgcat cggcgcatcc tgtacgcgat
     1081 gcacgacatg ggcttgcgcc ccgcatcgcc ttacaagaag tcggcgcgca tcgtcggcga
     1141 agtgctgggc aagtatcatc cgcacggcga tgtgtcggtg tacgacgcga tggcccgcat
     1201 ggcgcaagat ttctcgatgc gctacatgct ggtcgacggg cagggcaatt tcggctcggt
     1261 cgacggtgat cccccggcgg ccatgcgtta caccgaagcc cggctctcgc gcctggccga
     1321 ggagctgctg gcggacatcg acaaagacac ggtcgactgg ttggacaact tcgacagcac
     1381 gctcaaagag ccgtcagtac tgccgagctt gctgcccaat cttctgctca acggcgcgtc
     1441 cggcatcgcc gtcggcatgg cgaccaatat cccgccgcac aacctgggcg aagtggccga
     1501 cgcgatcgcc ttcctcatcg atcgctggga gtcgatcgtc gaagacgggc gcgcatcggc
     1561 gacggcagcc ggccagccgt tcggtcacga cgaagagaag gcgctgctgc gcgacctgtt
     1621 ttacgccgcc gacgacggtg tgacgatcga agacttgatg cagttcgtca aaggcccgga
     1681 cttcccgact ggcgcgctgg tgctgggtcg tgagggcatc gtcaacgcct acgccgccgg
     1741 caagggtcgc atcgtcatgc gcggcgtcgc caacatcgag gagatgaaag gcggccgcta
     1801 ccgcatcgcc atcaccgaaa tcccgtatca ggtcaacaag agcgcgctga tcgagcgcat
     1861 ggctgagctg gtgcgcgacg agcgcatttc cgacatctcc gacatccgcg atgagtccga
     1921 ccgcaacggc ctatcggtcg tcatcgagct gaagcgcggc tcgcagccga aaacggtgct
     1981 caacaagctg tacaaataca cggccctgca gtcgacgttc ggcgtgcaga tgctggcgct
     2041 ggtcgacggg cagccgcgcc tgctcagcct caagcgcgca ctgcagttgt tcgtcgagca
     2101 tcggcagagc gtgatcacgc ggcgatcgga gttcgatctg gcgaaggcgc ggcaccgcgc
     2161 gcacgtcctg gaaggcctgc gcatcgcgct cgccaacctc gacgcggtga tcaagactat
     2221 tcgcgagtcg ccggacgccg atacggcgcg cgcgcgcctg atggagcgct tcaagctgac
     2281 cgagatccag gcgcaggcga tcctcgacat gcagttgcgc cggctggccg cactggagcg
     2341 ccagaagatc gaagaagaat accagcaggt gatgcagacg atcgcgtacc tggaagacct
     2401 gctggcgaat ccgcgcaaga tcctggcgct ggtcaaagag gagacgatcg cgctgaggga
     2461 taaatatggc gatgcgcgcc gcacgcgtat cgccgccgag gcgaccgaag agatgtcgat
     2521 cgaggatctg gttgccgacg aggaagtgct gatccagttg accgagcggg gctacgtcaa
     2581 gcgcctgccg gtgaaggcgt tccgcgcgca agcccgtggc gggcgaggcg tcaccggcat
     2641 ccagacgcgc gaagaagacg ccgtggaata catcttcgcc acacgcacgc tgcaccacgt
     2701 cctgttcttc tcggacaagg gcaaggttta ccagttgcgc gcctacgaaa taccggacgc
     2761 cgatcgcacg gccaagggcg tgccgctcgt caatctcatc aacctgggcg agggtgagcg
     2821 tgtgaccgcc gcggtgtcgg tgcccgattt cgatccggcg acttatttca cgatgtgcac
     2881 tcgccacggg cgcatcaagc gcgtgtcagc cgaagagttc gagtcggtgc gccctagcgg
     2941 catcattgct atctcgctcg acgagggcga cgtactcggc tgggtcaagg tcacgcgtgc
     3001 ggagcaggac attatcatcg tcaccgagcg gggacaggcg ctgcgttaca acagctcgct
     3061 cgtccggccg atgggccgcg cggccgccgg cgtcaatgcc atccgcctcg gcgacggtga
     3121 ctgcgtgacg tccatggagg cgatcgtcgg ggatggcgag ctactcgtcg tgacggccaa
     3181 cgggttcggc aagcgcacac cgctcgagca atatccggcg aaagggcgcg ccacgggcgg
     3241 cgtgcgcacg ctggccaatc gcatcgacga gaccggcatc atcgcctcgg cgcgcgtcgt
     3301 gtggcccgaa gacgaggtga cgatcatcac ggccggcggc atcgcgctgc gcacggccgt
     3361 ggcgaatatc gcccgctccg gccgctcgac gcggggcgtg cgcgtgatgg cactgctgaa
     3421 cggcgacacg gtcgcgtcgg tggcgcgcat tgaaggcggc gagcgcgctg tggctgaatc
     3481 gagtgagccg atcgaagaag tcgctcccgt tgtgggcaac aacggtcagg aaccggttgc
     3541 cgaggcgacg aagtgagccg tgtcatgatt cagtcgccag catcagaact cactaaaacc
     3601 caacaacgcg cgctgaactc tttgccgccg cgtgtggccg aggccttgtc acggttttgt
     3661 gacgggctgc tggcgcgctt cggcgatcaa atcctgcgta gcatcctgta cggctcgttc
     3721 gcgcgaggcg aggcgcacgc cgagtcagat gtggacgtga tggttgtcgt ggggtgggag
     3781 ttcgagaagc tgccgggcgg atggtacgcc agtccgtacg gtgacccgcg ctgggaggcg
     3841 atcattgacc tatcggtcga ggctacgctg gactgcgagc ggtacgtgtc gcctctggtg
     3901 ctcagcgagg cgctgttcta cgacacatcg actgaagcgg cgcgagaagc acgtcgcgag
     3961 ggcatcgagt tgtacggtcc tgcctccggt gtaacgccgg ccgcccagta ccttgcgacg
     4021 ccgggagcgt ctgccggcgg aaccattcga tcggcaacac ctcgcgtcct caaggagaca
     4081 gaaccagcgc ctgactatga caccgagccg gctgatttgg ggaatccgcg cctgtggctt
     4141 gcactggcag acgagaagct gcgagcctcg cgcaggctgg ccgatgatga ctaccgcaac
     4201 gatgctgtat ctcgcgcgta ttacggcatg ttctatgccg caaaagcggc gctgctctca
     4261 gtgggcgtta ccgtgaagac tcatggaggc gtcaattcgg aattcggcag gctctttgtc
     4321 aagactgggc gaattgcaca tcgctatcag acaatgctca cacacgccag cgaggatagg
     4381 ctacgaagcg attatgcgcc caagactcgt cccagccagg agaacgtcga gcggatcatt
     4441 cgtgacgccg aagtcttcat caccaaagcg cgcgagctgg tagaagagga actgagcaag
     4501 cgaggcggtg cattgccgtg atcacgtcga ctgtgttgcg atccggcccg agtcaactgg
     4561 tcgaatacct gccggaagca gacgtcgatg cgctggccga gacgctcgtg gcgtttgcca
     4621 acagcgacgg cggcacggcg ctgctcggcg tggacgaaaa cggccggccc aacgggcgtg
     4681 tcttcccgga cgaagccgag accgctctgc gcgcggcgga ggcgcgctgc cgcccgtccg
     4741 tgcaggtgag atgggaacaa gtcgagatga ccggcgcggc ggtggtcgcc gtgcacgtgg
     4801 cgcgcagcac cgagctgcac tctttgggag acggccgcgt gctggtgcgc atgggcgccg
     4861 agaacagacc cttgggcggc gaggcgatcc gccaactggc ggcgacgaag agcagcggcg
     4921 atttcgagag tgaggcagtc cccggcgcga cccgcgccga tttggatgaa gcgatcatcg
     4981 ccgagtttgt cgagaagcgg gaagagcggc agcgcaagcg gctgacggtt ggcgtcgacg
     5041 agatgctggt cgagatcggc gcgctcgacg cggctggcca tcccaccacg gcgggcatcc
     5101 tgttgttcgg tcgcaacccc cagcagttca ttccgcagag cggcctggtg ttcgtgaagt
     5161 tcatcggcac cgagccgcgc ggcgagggcg gtctgccggg ctacggtcgc cgggaggaaa
     5221 tcggcggccc gctcgcgcgc attatcgaga ccgcctggga ggcggtgtgg gaagagatgc
     5281 acgttggcgc ggtggtgaag ggtctccagc gtgaagagcg aacggaatac cctccgttcg
     5341 ccgtgcgcga ggcgctggtg aacgccgtgt gccaccgcga ttaccgtctg aagggacgac
     5401 gtattgagat tcgcatgttc gccgatcgcc tggaggttat cagcccgggc gggctgcccg
     5461 gtttcatcac cgtcgacaac atcgtcgagg agcacttctc gcgcaacccg cgcatcgttt
     5521 cgggcctctt ccagtgggcg tacattgaag agttggggct gggcatcgac cggatgatcg
     5581 aggaaatgac gcaagccgga caccctccac cgcatttcaa ggccactcct tactcgttca
     5641 cggtggcgct gagcaacgct caggtgcgcc gcgctacgcc cgcctgggaa aaggtgatga
     5701 acgagcgcca ggcgcgcgcg ttggagtata ttcgggaaca cggccagatt acgaatcgcg
     5761 aatatcaaca gttgtgcgcg gatgtgacac ccgaaacact tcgcctcgat ctgtcggatc
     5821 tggtaaaccg tggaatcctt ctgagagtgg gcgcgaagaa gggaacgtac tacattatga
     5881 agtgaaaata tcccaggttt ttcccaaagt ggacttgggt aatcttatgt aaagagggtt
     5941 catgccgcct cttctatcgt gggtcgtagc cggactggct tctgcagcag cctacatagt
     6001 tctcgcgctc gtcctagtcc gtcggactgg cttccgagat tcgatcagcc gcgtggccgt
     6061 gctctatctc ggctcttcgg cgctgtggag cgtaggctac gccatttctg tttggcatgg
     6121 cttgaatccg agcgcccatc aactgggcgt cgaggctgcg ttcgacctga tctggatctt
     6181 tccacccctg atcctcatcc tcacgctcca gtttctggag cggcccggcg cgaagggcgt
     6241 ggcgatcatc ggcgccatgt ggattggctt tggtgtgctg gccggtctca acgtgttcgg
     6301 gttgcgcgag accctggtgc gatttcagat tagcgaaacg ttcaaggatg cgatcaaccc
     6361 cgtccgcatc atcggctggc tgagtttttc gatcagcgcg ctggcgctga cggttgccga
     6421 ctatcttcgc acgcagcgcc ctctccaccg caaccggatc atgttctggt taattgcgct
     6481 gctcctcatc caggtgggcg agggcctgac gctgatggag tcgcgccggg tggatcccca
     6541 gcagattggc ctcgtcgtcc gcctgttcgg cgccatcacg gcgagctatg ccatcttgag
     6601 ttaccatctg ccgaatctgc gctcgttcat gcgaggcgct tcggctgcag tgctgctgac
     6661 cctcgtggca ggcgcgctgt atctcgtcag cttcgtgctg ttccagtttg ttgttaacag
     6721 tcagttcggc cttgctctta ccgtggggct cacagcgggc atcgccctgg cgctggccgt
     6781 gattcagcag cctgtgctgc gcatcatacg aagcttgctg gatcgcttcc tgtaccgtca
     6841 gcaatacgac ccggcacagg cgttgcgcga atacagcgcc gccatcagca acattctcga
     6901 cctcgaccag ttggtctcgg tcgcagtcgg cattgtcagc gaggcattgg aagtgcggcg
     6961 cggcgcgctg atcctgatca ccgatctggc gaatggcaat acggatgtca ggctggtcaa
     7021 gggcatgggc ggcctcgagc tcgatcgctt cgagcttgac gccggcagcc cgatcctgga
     7081 cgaatggcgt acaggccgcc agccgctcac ccaatatgag atcgacatct tgccccgcta
     7141 tcgcagcgct tctgcggtgg agcgcactta tctccaggcg ctcgacatgg aagtctacgt
     7201 cccgattcgc tcgcagagcg tgctggtggg tatgttcgcc ttggggcgca aagattccgg
     7261 cgatccttat acgcccaccg atttcgacgt gctgatgacg ctgggcgatc agaccgccgt
     7321 cgcgctgcag aacgcgcgtc tggtgaccga tctgaagcga gctaacgcgg agatcaccca
     7381 gctcaacgac gagctgacca gcaccaatcg ccggctggag aagctggatc aggctaagac
     7441 cgatttcatc tcgatcgctt cgcacgagct gcgcacgccg ttgactcagg tgcggggcta
     7501 cgccgacatt ctcgccgact cggtgcgcga ggggagcccc agcctgccgc aaatggcgca
     7561 aatcagccag ggcattggac gcgccgcgct gcgcctggaa gagatcgtca cggccatgct
     7621 cgacatctct cagatcgacg cccaggcgct ggccatcaca cgcacgcctc tctcgataat
     7681 gtcggtgctg cgcttcgcca tggacaacta caaggaggcc gtcaaagcgc gctcgcagat
     7741 cgtgaccctg gagggcatcg acgacttgcc gcctgttcag ggtgacttcc agcgcttgtg
     7801 ccaggcgttc ctcaacgtca tcggcaactg catcaagtac acacccgacg gagggacgat
     7861 ttcgatcacg ggacggacgc tggcggggga gccgggtcgt gactcggctg ctttcgtgga
     7921 gatcgtgatc gccgactccg gtatcggcat cgacaaggcc gatcaagaac tgatcttcga
     7981 gaagttttac cgcgtcggtc ccgtcgagct gcactcgacc gggcagacga agttcaaagg
     8041 cggcggcccg gggctggggc tgccgatcgc gaagggcgtc attcaggccc acggcggcaa
     8101 gatctgggtc gagtcggagg gtcaggacga aacgcgcttc ccgggcagca cattccacat
     8161 tctcttaccg gccgcgccgg catccgttat tgcggagttc agggcacagt cgcaggcaga
     8221 agcagcccgc ttcacttccg cgccgtagca tcgtgacgct cgtcgtccgg gcgtctatcg
     8281 ctcaggcgat ccgcttcctt ctgggtcggc gggtcaagcg ccagcgccag tgcgccgagc
     8341 agtcccacgt tttctttcaa ttcggcgagg acgatcggaa cgacgaagcg ccgatccatt
     8401 acgcgccggc tcaccacgtc gcgtatgggc gcgaaaagcc ggtcgcccag gttggtgacg
     8461 ccgccgccga tcacgacgat cgatggattg aagatgtgca gaaacgacac gacgccgaac
     8521 ccgatcgcct cggcggcgtc gtggatcacc gaggccgcca gccggtcgcc tcgctccgct
     8581 gcttcgtgaa gcatctccac agtgatctct gaaaggttgc cgcgtgcctg gtcgcgcagc
     8641 agactgtatt cgccggccgc gatgcgctcg atagcgcgct gccgcatcgc cggcccgctg
     8701 gcgagcgctt ccaggcagcc gatattgccg catttgtcgg gccggcctct gtagtcgagc
     8761 gagacgtgac ctagctctgc gcccagccct ttcgagccga gcaggagttt gttgtcgatg
     8821 atcactccgc cgccgatgcc ggtgctgatg gtcaggtaca ccacgtcggc gtggccgcgc
     8881 gccgcaccgt agcgccattc cgccagcgcg gcgacgttcg cgtcgttgcc cacgtacgcc
     8941 ggcacgccga aaatgtcacg catgcgatcg cgcaagggca attcggcaag tgacgggatg
     9001 ttcaccacgc tgtacaggac acccgtgtac ggatcaagcg ggccgggcgc gcctatgccg
     9061 atggctgccg gcggcgagtc gggccacacg ctgcgggcgg tccggacaat gcggccgaac
     9121 accacgtcgg gcgggtcttc ggcgtgcgtc cattgctcgg cgcgcgcttc gatcgtcccg
     9181 tcggcgcggc agcgcgcggc gcgaatgcgt gtgccgccca gatcaatggc aatgtaggcg
     9241 tcgctcatgt cggctccaac acagagactg agggctagta taacgcattg ccaactcggt
     9301 taaaagccga tagaatcagc gacacatccg agtctggcgt tcgttaggag ccggttcacc
     9361 gcaagcgtcg ggcgcaggcg aggcaagcac agcgcatgaa tcacacgtat cgttatcccc
     9421 ctgtgttgcg cgcgccggcg ctgttcgttt ccctgctggc gggcggcatt ctgctcggct
     9481 cggtgatcgc cgagatcgaa gcggcagacg tgccggatgc gatcctgcac ggcgcgggca
     9541 tggtggcggg gatcgcgatg ttgtggcttg ggctggagtt cggcatgcgc cgcatcacgc
     9601 cgacgcgcga ggatatttcg acccggctgc ttcgcgaacg aacgatcccc tggcgcgagg
     9661 tgcgagacgt acgcgatggc ccgttcggcg tcctgatcat ccagccgaag cgcggactgc
     9721 cgatcgtaat ctggccctat ctggaagact tcagcgctct gctcgaagct gtaacgaact
     9781 ttagcaactc ctccacgccc tcgacatcat gactggagtc tggcgaattg ttctatccac
     9841 tgcgctggca atcttggccg gctgttcggg atcgacgcgc gtggtcatgc ctacttccgc
     9901 aggtgtggcc acaccgcacc cggacctctc ggttttcgtg gataccgtgt attatcgggt
     9961 tgccgggtcg acggcagatg aactacgtgt gcagatggat cgatccaggc cggggaattt
    10021 cgatgcgcac acggagtggt acgtgagctg gtcgtacccc tactcagagg cggaaggcag
    10081 ttgcaccacc gggccggtca ccatcagcgt gaacatcaca ttcacccttc cgcagtggaa
    10141 cacaccggcg aatgcctcgg aagaccttgt tgatagatgg aatgcgtatc ttgccgcgtt
    10201 gcaggttcac gaggatgggc acaagggcat cggcatcgaa gccgcgaacg agattctaca
    10261 agcgatgaac gcgcttccgg cgtatcgttt ctgtgaggag ttgaagcagg cagccgatgc
    10321 agccggcgag cgcgtgctca gcgagtaccg ccaacgcgag gcgatctacg atcagacgac
    10381 gaatcacggt gcgactcagg gcgcgcggtt tccgtgaggc gtgcttggag gcgatccgcc
    10441 gctgtctcgg cccacccgcg agcgacggtg tataatcgtg ttcataatcc cgggcctgcc
    10501 caatagacat gcgtcatgcc taaaacaaac agcgtggtga acgacaaaga gcgtcatatg
    10561 ttgatcgtga agttcaagga cggcgcgtca gccgagctgc cgtgggacgt gctgcgcgac
    10621 gcctgcccct gtgccgaatg caaggacctg catggcacag gcgatccgct caggttgacc
    10681 gccatgccga acaagacgct caccgggttc gaatacgccg ggaactacgc cgtgatactc
    10741 atctggggcg acgggcaccg cttcggaatt tacacctggc cttatctgcg cgagctggct
    10801 cagaagctgg ccgcttcgct gtcgtagcgg tagaatgtga tcgaccctcg ctttctcgac
    10861 acccttcgca agatgcacac gcggctggcg agcacggatg tgaactgggt cgtcaccggc
    10921 agtctgggct tcgccctgca gggcatgccc gtcgagcctc acgacatcga catccagtcc
    10981 gacgaaaagg gcgtctacga gatcgaacgg ctcttttcag agtatgtgac tcgcccggtg
    11041 gcgctgtctt cgactgaaac gatgcactca cacttcggcg ctctctcgat cgacagcctc
    11101 aaagttgaga tcatgggtga tattcagaag cggctgtcgg atggaacgtg ggaagcgccc
    11161 gttgatctga atcgctacaa acgctttgtc gagattgaag gaatgaggat ccccgtcctg
    11221 tcgctggagt acgagtacca ggcctacctg aagctcggtc gtattgagag ggcggagatg
    11281 ttgaagaatt ggctgctgga catgtcaaat gacggatgat ctgaccccga tccgcaagca
    11341 atatctcgcc ctcaagaacc agtatcccga cacgatcctc ttcttccgcc tcggcgactt
    11401 ctacgagacg ttcgacgccg acgcagagac cgtcgcgcgt gagctggaca tcgtgctcac
    11461 ctcgcggccg gtcggcaagg gacagcgcgt gccgctggcc ggcgtgccac accacgcggt
    11521 cgatcgctac ctggcgcagt tgatcgagaa aggcttccgc gtcgccgtcg cggagcagat
    11581 ggccgacccg tcggcggtga cgggcatcgt cccgcgcgaa gtgacgcgtg tcgtcacgcc
    11641 gggcacgatc ctcgatccgg agatgctcga cgagaagcgc gcgaactacc tggcggcgtg
    11701 ggtcgagcac gatggccgcg ttgggctggc gcactgcgac atcacgaccg gcgagttcgc
    11761 cgtcgcgcag gtcgactcga atgccgccgc gctgcaggaa ctgctgcgca tcgcgccgcg
    11821 cgaatgcctg cacccccaat cgcaaagcgc gccggcgcag gcctcggcgg cgattcactt
    11881 cacgccgctg ccggactgga agttcgaggc cgcgaatgca cggcaggcgc tgctcgatca
    11941 tttcgacgtg gcgacgctgg ccggcttcgg catcgacggg cagtcgctcg cgattcgcgc
    12001 ggcgggcgcg atttgccagt atctgcgcga gacgcagccc ggcgcgctcg cccagttgtc
    12061 gagcctgcgc tcgtactcga ccgggcgctt catggcactt gacccggcga cgcgccgcaa
    12121 cctcgagctg accgagacga tccgcgagcg caagacgcaa ggctcgctgc tcggcgtgct
    12181 ggacgcgacg cgcacggcga tgggcgggcg cctgctgcgc gcgcacatct cgcagccact
    12241 gctggatcga gcggagctcg aagcgcggct ggatcaggtg caggcatttt tcggcgatgc
    12301 gttgacgcgc gccaaggcgc gcgagacgct caaggggatg cccgatctcg agcgcgccgt
    12361 gggccgcgtc gtcggcggca gcgccgcgcc gcgcgacatg gtcgccatcc gccgcgcgct
    12421 cgagctcgtc ccggaattgc aagagtcaat ctccaatctc caatctccaa tctccaacct
    12481 ccagatcgat ccgtgcgccg acgtcctcgc gatgctgcaa gcggcgattg ccgacgagcc
    12541 gacaacaccg cccggcctga tccggcccgg ttactcggcc gagctggacg gcatcctcag
    12601 cgcctcgcgc aacgcaaaag agtgggtcgc cggtttggag aagaccgagc gccagcgcac
    12661 cggcatccac acgctcaagg tcggttacaa caaggtgttc ggctattaca tcgaagtgac
    12721 gaacgcaaat cgcgacgcgg tgccggacaa ctacatccgc aagcagacac tgaccaacgc
    12781 cgagcgctac atcacgcccg aattgaagga gtacgagaca ctcatcctca acgccggcga
    12841 gcgcctcgtc gaggtcgaga accgtctgtt caaggaaatc tgcgcgcagg tgtcgagccg
    12901 cgcccgcgcc ctaaggatgg cggcgcaggc catcgcgcag atcgacgtcg cgtccgcgct
    12961 ggccgaggtt gccgcgctca acgcctacgt ccggcccgaa ttgattgacg ccgacgagat
    13021 cgacatcgtc gcgggacggc acccggtcgt cgagcgcacg ctgagcgaga cgcgcttcgt
    13081 gcctaacgat ctgaaattgg gcgccgacac gcgcatcttg atcatcaccg gcccgaatat
    13141 gagtggcaaa tcgactttcc tgcggcaggc cgcgctgatc gcgctgatgg cgcagatcgg
    13201 atcgttcgtg ccggctgagc aggcgcgtgt cgggttggtc gatcgcatct tcacgcgcat
    13261 cggtgcgcag gatgagatcg ccgccgggca atcgacgttc atggtcgaga tggtcgagac
    13321 ggcgaatatc ctgcatcacg cgtcggcgcg cagcctgatc gtcctcgacg agatcgggcg
    13381 cggcaccagc acgtacgacg ggctgagcat cgcctgggcc gtcgtcgagt acgtccacaa
    13441 ccatccgcgc ctgaagagca agacgctgtt cgctacgcat taccacgaat tgatcgcgct
    13501 ggccgagacg ctgccgcacg tgcgcaacgt caacgtcgcc gtcgcggaag agggcgaccg
    13561 ggtggtgttc ttgcacaaga tcgtcgaggg cggggcggat cgctcgtacg gcatccacgt
    13621 cgcgcagctc gccggactgc ccaagccggt ggtacatcgc gctgaagaga tcttgcagca
    13681 gttggagcaa ggcgaccccg cgcacgcgcc ggtgtctggc gtgcagcagt tgtcgctctt
    13741 cgcgacggaa agcccggcgc tggaagagct gaagtcgatc gacgtggacg cgctgtcgcc
    13801 gctggaggcg ctgacaaagt tgtatgagtt gaagaagaag gcgtcaacgg attagcagcg
    13861 aatcaacgaa tcggcgagtc agcgatcttg cctgttccgc cggtgcagtg actcgtgaag
    13921 gagaacagga tgagtccgac acaagacgtc taccgggagt tgaaggcgct cacgcgccag
    13981 cggatacgcc tgatctggga gaaagcacaa ttgggcgggt ctctctccga tgaggatgcg
    14041 cacctggtcg aggccatgcg cgagcattcg gagtacaccg acctgtgggg gcaactcgac
    14101 catttgtctg acgaacagat cgagcgagac gggacgaatc cgatcctgca cgtgattatc
    14161 caccagacca tcgagaacca gatcgccggt ggagagcctg agcaaacggg ccggaccgtc
    14221 gaggcgttga tgcggcaggc cctgtcgcgg catgaagcta gtcaccgtgt tggagaagtg
    14281 ctggcgggcg aaatctggca catccttaaa gaggatcgtc ccttcgacga ggcgggcttc
    14341 gtccgcaagc tgcgccggct ggcgaagcca ggggcagcgc attcggacca tgcgccgcgc
    14401 ccgcatcggc gtcgccccag cagaaagcgt tgatgacttt ctctacagca tgaccccgcg
    14461 ctagacgacg atgcggagca gtagtatcat ccttccgcaa gttttccccc atgcggcgct
    14521 ggcggtgagc gaagtcatcg gataggcact tgttggcgtc gccgcctcac gtactcgtgc
    14581 ccgacggctc gcccgacggt tggtcctggg ccgcaggctg cggcgaggac ggcggctgcg
    14641 cgctggtgac cggcgccaca gccgggacca tgcgccgcgt ctttggagtc gtgtaccccc
    14701 acgcgatcag cccgatcgca atcccgcctg cgaccccgcc cgccatcaag aagccgttcg
    14761 cgaccgtgtc gagcgcagtg cgcgccacgt ccagcaaggc cagccgaaac gagtcgctgt
    14821 actcgacgtc gccgaagcgc gcctggttca gtccgaagcg cacccacccg gcgctg
//
LOCUS       DBNHXB010000348         4344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4455693, whole genome shotgun sequence.
ACCESSION   DBNHXB010000348 DBNHXB010000000
VERSION     DBNHXB010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4455693"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="AC162G_14180"
     CDS             <1..1071
                     /locus_tag="AC162G_14180"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077001.1"
                     /translation="AELPPILTDTVPLSVSLVSISDGGVAQTKGVTPTRTVISWTLPA
                     LSPGDTAVRSFMVRVADNLVSGTQIVNQDYRVTWTETETSTLFSNAGQPVTTTVREVG
                     LIDSYKEVTPTWALPGPNNVLTYSVHVVNSSAVSLTGVTLRDVLPWQFSTYQRDAVAS
                     AGSVVSDIVTVRWTGNVDAFSSQVVTFTVRVDPGYRGPVTNTATISHSSLLTPVVVQA
                     VAYITDLPVLFISKSASPDPVAAGSDLNYTIRVVNAGQQATGLVITDVIPTNTTYVAG
                     SASANGQLVGNQLRWEVPVLKSGESQALAFRVKVESGQEVVNRLYAVKSAEGITSLGA
                     PVVTRVTRGNKIYLPIVMRNAP"
     gene            complement(1244..3256)
                     /locus_tag="AC162G_14185"
     CDS             complement(1244..3256)
                     /locus_tag="AC162G_14185"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR5077002.1"
                     /translation="MLTTVEKILFILLALVSVYFAFATFVRIRRIIQRGTGRLATDRF
                     VSRLVKSALVFVTQYPTWKRRPLASLAHAGVAFAFVLYLLVNVGDGLEGFLPNFIFLG
                     EDTVGDVYRLIADVLSVAALSGMVALLLRRAITGRKIWNFRSSTLLHPKADFGIKRDS
                     LIVGVFILFHIGTRFLGASVQIAATEADPWQPFATSTSALWAGLTPQMLEVMEHVFWW
                     GALGSILLFVPYFLYSKHIHIVMAPLNFLLKPRRASIGQLDKIDFEDESIQQFGASRL
                     EHLSQAQLFDAYACIMCNRCQDACPAYATGKALSPAALEINKRYQINQEGAALAAGKE
                     SSLGLLEFAISAEAVWACTACGACVEVCPVDNEPMRDILDIRRSLVLMDSAFPAQLQQ
                     AYKGMERQGNPWSISPDARLDWAKGLNVPTIEQNPEPDVLWWVGCAPATDARAQKTAR
                     AFATLLNEAGVSFAVLGKMERCTGDSARRSGNEALFFELATGNVEMLNEVKPKRIVTA
                     CPHCLHTLANEYPAYGGNYEVIHHTQLIEELLLLGKLHVDSRRQAEVAFHDPCYLGRH
                     NGVYDTPRSALVQANVSVAELPRNRSRSFCCGAGGAQMWKEEEHGAERVSANRFREAM
                     ATGKDTLAVGCPFCMIMLTDAAKAEKSEMQVKDVAEIVEEALARHG"
     gene            complement(3299..4138)
                     /locus_tag="AC162G_14190"
     CDS             complement(3299..4138)
                     /locus_tag="AC162G_14190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014571.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034069 - aminoglycoside
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HIR5077003.1"
                     /translation="MASNSVGVRDIFRGLRSLGLTLPSGSAVIVHASLSSFGYVRGGA
                     RAVLAALLWTCDTIVLPAFTYQSMVWPLVGPPDNGMTYGHPEFDAANRNATFFHRNLP
                     AHRDMGVIAETLRRHSIAVRSSHPVLSFVAAGKRAGEVLAGQTLDDPLAPIEWLQRHD
                     GDVLLLGVGHTRNASIHLAERLAGRRQFMRWAIVRGEDSRDARAVRLPGFPGCSDGFD
                     AIEPEIARATRQVTIGGAVVRRVPLSMLIPIAAGLIADQADALLCNRPDCERCSAVRR
                     AGR"
     gene            complement(4168..4338)
                     /locus_tag="AC162G_14195"
     CDS             complement(4168..4338)
                     /locus_tag="AC162G_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR5077004.1"
                     /translation="MAIRLAKEAVNKSYEMPLADGLDYERRLFYFLFATEDQKEGMQA
                     FVDKRPAEWKGR"
BASE COUNT          786 a         1403 c         1403 g          752 t
ORIGIN      
        1 gcggagctgc ctcccatcct gacggacaca gtccccctga gcgtgagcct cgtgagcatc
       61 agcgatggcg gcgtggctca aacgaagggc gttacaccga caagaaccgt catctcttgg
      121 acgctgcctg cgctaagccc gggagacacg gcagttcgtt ccttcatggt gcgggtggcc
      181 gacaatctgg tgagcggcac gcagatcgtc aaccaagatt atcgtgtgac ttggaccgag
      241 actgagacca gcacgctctt ttctaacgct ggtcagccgg tcacgaccac cgttcgagaa
      301 gtggggctga tcgattcgta caaagaggtc acgccgacgt gggcactgcc cgggccgaac
      361 aacgtgctga cctattccgt gcacgtcgtc aattcgagcg cggtttcgct gaccggcgtc
      421 accctccgcg acgtgctccc ctggcagttc agcacctacc agcgcgacgc ggtggcgagc
      481 gcaggctcgg tcgtgagcga catcgtgacc gtacgctgga cagggaacgt ggacgccttc
      541 tcgtcccagg tcgtgacctt caccgtgcga gtggatccgg gctaccgggg gccagtaacc
      601 aacaccgcga cgatcagcca ctctagcctg ctcaccccgg tcgtcgtcca ggcggttgcc
      661 tatattaccg atctgcccgt gctgttcatc agcaagtcgg cttcgcccga cccggtggca
      721 gccggctcgg acctcaacta caccattcgc gtggtcaacg cgggccagca ggcgaccggc
      781 ctggtcatca ccgacgtgat cccgaccaat accacttacg tcgccggcag cgccagcgcg
      841 aacggccaac tggtggggaa tcagttgcgg tgggaagttc cggtgctcaa gtcaggagaa
      901 agccaagcac ttgcattccg agtgaaggtc gagtccggac aggaggtagt caaccggctt
      961 tacgccgtca agtcggcgga aggaatcacc agcctgggcg cgccggtggt cacacgtgtc
     1021 acccggggaa acaagattta tctgcccatc gtcatgagaa acgcgccgtg atacggcggc
     1081 tcaaccggct tggagcgttc tgcgcccact ccgtttccgc ggagtgggcg cagaatcatt
     1141 ttactcggat cgtttgttcc cagtggatcg tcaatctggt tttgacggat agtcgtagta
     1201 gcgacttcag tcgctgacaa ccaaagtcgt caccacgatc catttacccg tgtcgcgcaa
     1261 gcgcctcctc cacgatctcg gccacatcct tcacctgcat ctcgctcttc tcggccttgg
     1321 ccgcgtcggt gagcatgatc atgcagaacg ggcagccgac ggcgagcgtg tccttgccgg
     1381 tcgccatcgc ctcgcggaag cggttcgcgc tcacgcgctc cgcgccgtgc tcttcctctt
     1441 tccacatctg cgcgccgccc gcgccgcagc agaaagaccg gctgcggttg cgcggcagct
     1501 cggcgacgct cacgttcgcc tgcaccagcg cgctgcgcgg cgtatcgtag acgccgttgt
     1561 ggcggccgag gtaacacgga tcgtggaacg cgacctcggc ctgcctacgc gaatcgacgt
     1621 ggagcttgcc gagcaggagc agctcctcga tgagctgcgt gtggtggatc acttcgtagt
     1681 tgccgccgta ggccgggtac tcgttcgcca gggtgtgcag gcagtgcggg catgccgtga
     1741 cgatgcgctt cggcttgact tcgttcagca tctcgacgtt gcccgtcgcc agctcgaaga
     1801 acagcgcctc gttgcccgag cggcgggccg agtcgcccgt gcagcgctcc atcttgccga
     1861 ggacggcgaa gcttacgccc gcctcgttca ggagcgtagc gaaggcgcgc gccgtcttct
     1921 gcgcgcgtgc atcggtcgcc ggggcgcagc cgacccacca caagacgtcc ggctcggggt
     1981 tctgctcgat cgtcggcacg ttcaagccct tcgcccaatc gagccgcgca tcgggcgaga
     2041 tgctccacgg gttgccctgg cgctccatgc ccttgtacgc ctgctgcaat tgcgccggga
     2101 aggcgctgtc catcagtacc aggctgcggc ggatgtcgag gatgtcgcgc atcggctcgt
     2161 tgtcgaccgg gcagacttcg acgcacgcgc cgcacgccgt gcacgcccac accgcttcgg
     2221 cgctgatcgc gaattcgagc agcccgagcg acgattcttt tccggcggcg agcgccgcgc
     2281 cttcctgatt gatttgatag cgtttgttga tctccagcgc ggcgggcgag agcgccttgc
     2341 ccgtagcgta tgccgggcag gcatcctggc agcgattgca catgatgcag gcgtacgcgt
     2401 cgaacagttg cgcctgcgaa agatgctcca gtctcgacgc gccgaactgc tggatgctct
     2461 cgtcctcgaa gtcgatcttg tcgagctggc cgatcgacgc gcgccggggc ttgagcagga
     2521 agttgagcgg cgccatgacg atgtgaatgt gcttcgagta aaggaagtac ggcacgaaca
     2581 gcaggatcga gccgagcgcg ccccaccaga agacgtgctc catcacctcg agcatctgcg
     2641 gcgtgagccc ggcccataac gcgctcgtcg acgtggcgaa gggctgccag gggtccgctt
     2701 ccgtggcggc gatctggacg ctcgcgccca ggaagcgcgt gccgatgtgg aacaggatga
     2761 agacgccgac gatcagcgaa tcgcgcttga tgccgaagtc agccttcggg tgcagcagcg
     2821 tgctgctgcg gaagttccag atcttgcggc cggtgatcgc ccggcgcagc agcagcgcga
     2881 ccatcccgga gagcgcggcg acgctcagca cgtcggcgat caggcggtac acatcgccga
     2941 cggtgtcttc gcccagaaaa atgaaattcg ggaggaaacc ctccagtcca tcgccgacgt
     3001 tgacgagcaa atacaagacg aacgcgaacg ccacgcccgc gtgcgcgagc gaggccagcg
     3061 gtcgccgctt ccaggtggga tattgcgtga cgaacaccag cgccgacttg accaggcggc
     3121 tgacaaaacg atccgtcgcc aggcggccag tgccgcgctg gataatgcgg cggatgcgga
     3181 cgaacgtggc gaacgcaaag taaaccgaga ccagcgccaa cagtatgaaa agtatctttt
     3241 cgacggtggt cagcatgatc cctgtcctat gtatcgaaaa cgctccggcg ccggtggttc
     3301 atcgcccggc gcggcgcacg gcgctgcagc gctcgcagtc gggccggttg cacaatagcg
     3361 cgtcggcctg atcggcgatc aggccggccg cgatcgggat cagcatcgac agcgggacgc
     3421 gccgcacgac agcgccccca atcgttacct gccgggtcgc gcgcgcgatt tccggctcga
     3481 tcgcgtcgaa tccgtccgag cagccgggga agccgggcag gcgcacagcg cgggcatctc
     3541 tggaatcttc gccgcgcacg atcgcccagc gcatgaactg cctgcgcccg gccagccgct
     3601 cggcaagatg gatgctcgca ttgcgcgtgt ggccgacgcc gagcaggagc acgtcgccgt
     3661 cgtgacgctg cagccattcg atcggcgcca gtggatcatc gagtgtctgg ccggcgagaa
     3721 cctcgcctgc acgcttgccg gcggcgacga acgacagcac gggatgtgaa ctgcggacgg
     3781 cgatggaatg ccgccgcagc gtttcggcga tgacacccat gtcgcgatga gcgggcaggt
     3841 tgcgatgaaa gaacgtcgcg ttccgattgg cggcgtcaaa ctcaggatgt ccgtaggtca
     3901 tgccgttgtc gggcgggccg acgagcggcc acaccatcga ctggtaggtg aaggccggca
     3961 gcacgatggt gtcgcatgtc cacagcagcg cggcgagcac ggcacgcgcg ccgccgcgca
     4021 cgtagccgaa tgacgacagc gaggcatgaa cgatgacggc gctgcccgaa gggagcgtca
     4081 ggccgagcga tcggagtccc ctgaagatgt cacgaacgcc gacggagttc gaagccatac
     4141 gagcctctgt ggtcgaagcg ttcgtcgtca ccggcccttc cactctgccg gccgcttatc
     4201 gacgaacgcc tgcatgcctt ccttctggtc ttcggtcgcg aacaggaaat agaacaggcg
     4261 gcgctcgtag tcgaggccgt cggccagcgg catttcgtat gacttgttga cggcctcttt
     4321 cgccagccga atcgcgatcg gcgc
//
LOCUS       DBNHXB010000349        25689 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1771702, whole genome shotgun sequence.
ACCESSION   DBNHXB010000349 DBNHXB010000000
VERSION     DBNHXB010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 25689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 25689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25689
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1771702"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(394..873)
                     /gene="bfr"
                     /locus_tag="AC162G_14200"
     CDS             complement(394..873)
                     /gene="bfr"
                     /locus_tag="AC162G_14200"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HIR5077005.1"
                     /translation="MKGNDKIIEVLNQLLADELTAINQYMVHSEMCDNWGYDSLHKAI
                     EGQARDEMHHAEWLIQRIIFLEGMPVVTRLNPMKIGKIVPEMVGNDEEAELGAVHAYN
                     SAIRLAHEVDDQATVDLLTKIVKMEEGHVDWAEKQRAQIEQMGLENYLTNQTEGAGG"
     gene            complement(974..1453)
                     /locus_tag="AC162G_14205"
     CDS             complement(974..1453)
                     /locus_tag="AC162G_14205"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR5077006.1"
                     /translation="MAILDTLEHAGDAHSMPGSLPTHYRYTAGVAGQRFLAALRDEGV
                     ILGARCDKCRYTYVPARMYCERCFAALPESAWVKVGLGGTLVSFTVVHVDLDGKPLDA
                     PRTLGLVRLDVADGTLVHDLGEFSAQAPRVGMRVTAALKPKAERTGSILDIRYFRPE"
     gene            complement(1575..2081)
                     /locus_tag="AC162G_14210"
     CDS             complement(1575..2081)
                     /locus_tag="AC162G_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR5077007.1"
                     /translation="MSAETHVFPTTSLSQDDLDENRVVTVDWTPHAEYAWDEGVAIGG
                     YLQGLKEGKLLGRVCRKCRRRMIPPRMFCEQCFRPTDEWFEASDTGTVRTFSLCYVTW
                     DMIKLDRPQIPAVIDIDGASPGMGIMHLLGEVDPKAVHVGMKVQAVWKPPEQREGAVT
                     DILHWKPL"
     gene            complement(2156..3322)
                     /locus_tag="AC162G_14215"
     CDS             complement(2156..3322)
                     /locus_tag="AC162G_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014288394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HIR5077008.1"
                     /translation="MGMRRVAIVGAGMTQFVRRSLETGRELAWEAASKALESCEMTLD
                     DVESVVMGTAPDAFDGVHMKSEWLSDGAGAWGKPYMRGYVGGGTGVFAVAQGWYHVAS
                     GLFDTCLVVCEEKMSPCRPHPQAAFISIFDNFTERPLGPNLLWIFALEMNAYMQRYGL
                     KKEDIARVAVKNKRNAADHPAALLGDADITVEDILSSEVLAWPVQRLDVSPVSDGAVA
                     VVLAADNVARRVTNKPVWIEGVGWNLDTAYWTNRDLSYPTYVEEAARMAYKMAGITEP
                     RKQIHIAEPYDPFDYKELHHLEGLLLFDKGKAPEATALGVTQRDGDLPVCPSGGLLGV
                     GNPIAAAGLMKIAELFWQLREEAGARQVPGKPRRGVAQAWGDLMQVGTVVVLGK"
     gene            complement(3384..3827)
                     /locus_tag="AC162G_14220"
     CDS             complement(3384..3827)
                     /locus_tag="AC162G_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5077009.1"
                     /translation="MSELRHAKSFRDLVVYQKARQLATEIFRVTKDFPREEMYSLTNQ
                     IRRSSRAVGAQIAEAWAKRRYEAHFVAKLTDADGEQQETQHWIETAADCDYLTRSQAG
                     DLVDKCAEVGRLLGGMMAKADQFCGTSFAAIREPVTEYFVGGRDN"
     gene            complement(3901..5055)
                     /locus_tag="AC162G_14225"
     CDS             complement(3901..5055)
                     /locus_tag="AC162G_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR5077010.1"
                     /translation="MSRKVAVVGIGWSGFRPITPELSYKELAFEAAARAYADAGIDPR
                     RDVQGFVTAAEDYHEGTSIFDEYTPDQLGAALRPMHTIAGDGLHALAAATMQIASGLM
                     NVVVVEAHSKVSNILSLSHISAFALDPVVERPLAAHPSFVAGLEMQWLLSAGRFTLPQ
                     AAAVAVKNKRNALANPAASYAANVKLRDVLDAAPLAEPVTRLMAAPHADACVVMVLAD
                     EDTARGLRGKPVWIRGLGWANDTPALDSRAWGEARYARKAGDMAYRTAGIRSPRSEID
                     FAEIDDTFAHKELVHLEALRLAREGEAGAWTAEGGTQRSGEFPVNPSGGSLGVGALLD
                     ATGLARALEVVLQLRGEAGARQLPKAVTGLAFAWRGLPTTSGAAAVFSNQ"
     gene            complement(5189..5401)
                     /locus_tag="AC162G_14230"
     CDS             complement(5189..5401)
                     /locus_tag="AC162G_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009632997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR5077011.1"
                     /translation="MTESGKFIKLDQFLKWQGIVDTGGQAKQLIQEGRVRVNGQVERR
                     RGRKLVNGDTVTVGGTILVVNVDRDT"
     gene            complement(5478..6362)
                     /locus_tag="AC162G_14235"
     CDS             complement(5478..6362)
                     /locus_tag="AC162G_14235"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016297 - fatty acyl-[ACP] hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HIR5077012.1"
                     /translation="MPRVHTRRFRVRSTDCDAIGQVNHAVYVRYMQEAAIEASSDAGY
                     TPEWYIEHGVGWLIRRSRITYFQPARYGDELDVMTYVVNFRRVRSQRNYDITRVGDNL
                     LIVRAATDWVFVDERTGAPRRIPDEVPAIFLPDGGDPAAEIIADLPHPPDEPPPNAFH
                     VTRRVNYYDMDENQHVNNAVYLNYLEQATIDAAASAGFDLPRWLDLGGMFVVRQHDIE
                     YLHPAKYGDTLDILTWVGETSPPSVVRHTVMRDQDGTLDIRAQTQWVWIDLASQKPAE
                     IPPALLEALAGRDVKREA"
     gene            6596..7363
                     /locus_tag="AC162G_14240"
     CDS             6596..7363
                     /locus_tag="AC162G_14240"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR5077013.1"
                     /translation="MSQSPKLLLAALLLSLALVSPAVQPVAADTTSSLSPYWGQTIAR
                     WEDLIAHYAGQRGLDPDLVAAIIFEESHGLTNRISVVGAVGLMQIMPKETGFSWRPTA
                     VELLDPGVNLYWGTRTFAQVVQQAEGGLSRALAAYNGGWEQEHLRATQLFAGKVLDHY
                     ARAIAARAGFNARSMKKWSLVLDIQTSAGLKRIQTIRSDGTIDLDAEYDLAKLPASTP
                     HAMTYSAIDAGGAAWLVEAWVIVEPIEGGTPNGRGTY"
     gene            complement(7458..8672)
                     /locus_tag="AC162G_14245"
     CDS             complement(7458..8672)
                     /locus_tag="AC162G_14245"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmpA family aminopeptidase"
                     /protein_id="HIR5077014.1"
                     /translation="MRPRTRDCCILIGALPTGTHNAITDVPGVKVGHSTLIEGEGALI
                     PGSGPIRTGVTAVWPHSGDLFYEKVAACVHTINGFGEVTNSEQVHEMGVVEGPILITN
                     TLNVPRVADYAIDWAFEHYPQMGITDWGISPVVAETSDMYLNDIRGRHVQREHVFAAI
                     DGATAGPVEEGSIGGGTGMSCLGFKGGIGTASRIVGIGDRRFTVGVLVQSNFGRRGDL
                     RIDGVPVGRELLTWLDEADRQHIAQHPERNEVESKGAAPHSNSHSNSIIIVVATDAPL
                     TVRELRRVAVRASFGLARVGSMGGTTSGDFVIAFSNANRVAHRPDSSVYEWHALAEMV
                     TRERPDDPPPISPFFIAAIEATEEAILNSMFKSPTMVGRDNHVRHGLPIEETVHIMRR
                     YGHTRAHSPRST"
     gene            complement(8742..9983)
                     /gene="lysA"
                     /locus_tag="AC162G_14250"
     CDS             complement(8742..9983)
                     /gene="lysA"
                     /locus_tag="AC162G_14250"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR5077015.1"
                     /translation="MLQTHSGRATCEEMKLADLAARFGTPLYVYSRRRLKANTERVAR
                     AFAPLRARLRYSVKANSNGAILRLLREWGAGFDVVSAGELYRSLQAGASPGAVVFAGV
                     GKSDAELRYAVETGVGWINVEAREEVDVLEAVAASMVARPHVAVRLNPAVEADTHRHI
                     ATGGKRSKFGIDFDEAGRLLADAARYPHLDLAGVHVHVGSQLASPDATVAAVRKALEL
                     VDRYRLRMLDIGGGFPVNDQAGADAGLVPAPSDFAEALAPLLRDRDLELMIEPGRSII
                     ADAGLLLARVLYVKRRAGRQVVVVDAGMTDLIRPALYDAYHPIIPLRAHSATGVLSDV
                     VGPVCESADAFAHDRPLPPMQRGDLLVITHAGAYGMSMASNYNSRLRPAEVLVDGASA
                     SLIRRRETMEDLLGAEVINNT"
     gene            10224..10610
                     /locus_tag="AC162G_14255"
     CDS             10224..10610
                     /locus_tag="AC162G_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5077016.1"
                     /translation="MVATILIAEDERDIRDLVVFSLRFGGFEVVEATNGQEAVEKAQQ
                     VMPDLILMDVRMPRMTGYDACKQLKSMEDVKHIPVIFLSAKGQEQEIQQGMEAGAEEY
                     ILKPFAPEDLVNMVRKVLQKTGAGSP"
     gene            10638..11447
                     /locus_tag="AC162G_14260"
     CDS             10638..11447
                     /locus_tag="AC162G_14260"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5077017.1"
                     /translation="MSVIIIDNGLVHYEAIGRGRPVIFLHGWLGSWRYWMPTMEAISD
                     RHRTYAFDLWGFGDSDHSPQRYDLGAYEQQLGQFIENMGLGETSVSLVGHALGAAIAV
                     RYANRVRSPNQPVLRIMAVSLPLGPHAIDRRLAPGGGSLLDRVLGRRGAADYPEVDLE
                     AQKADPNAIMMSARLLATTDQETARAMERLDIPLLLVHGEKDPLIALPSPDSLQVSDQ
                     NVKPITLSDSRHFPMLDEAPKFQRLLKDFLETRPEDLQTLALKEEWRRRTY"
     gene            11558..11902
                     /gene="trxA"
                     /locus_tag="AC162G_14265"
     CDS             11558..11902
                     /gene="trxA"
                     /locus_tag="AC162G_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015164786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR5077018.1"
                     /translation="MAKPSVVTDMTFDGEVLKSHVPVLVDFWAVWCGPCRMIAPIVDD
                     IANEYDGKIKVMKLDVDENQTTAMSYGVMGIPTLILFKGGKAVERITGYMPKERLLSR
                     LLPHLDATPSVN"
     gene            12302..13297
                     /gene="trpS"
                     /locus_tag="AC162G_14270"
     CDS             12302..13297
                     /gene="trpS"
                     /locus_tag="AC162G_14270"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR5077019.1"
                     /translation="MNKPRILTGDRPTGPLHLGHYVGTLVNRVRLQDSHELFLVVADY
                     HTLTTQPTREKVARTREHARAIVLDYLSVGIDPERATIYVQSDVPEVAELTLLFSMLV
                     TVPRLERVPTLKEVMREQNITHPSMGLLSYPVLQAADILLVHADLVPVGGDQESHVEV
                     TREIARRFNQLYGAVFKEPQALIGDVPMLVGIDGKLKMSKSLGNAIYLSDDAETVSAK
                     VMQMYTDPARIHADVPGAVEGNPVFTFHDTFNEDHAEVENLKARYRSGKVGDVEVKQK
                     LARALNRFLDPMRARRAGYGERPSLVDDILEAGASRTRPIARETLRKAREAMGIS"
     gene            complement(13320..13964)
                     /locus_tag="AC162G_14275"
     CDS             complement(13320..13964)
                     /locus_tag="AC162G_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HIR5077020.1"
                     /translation="MLVKERMTRQPILASPDLPISEALNLMKKERIRRLPVVDKNGHL
                     IGIVSDKDLLHASPSPATSLSVWEVTYLLGKIKIEDVMTKKVITVTEDTPLEDAARIM
                     ADNKIGGLPVVKDATVVGIITETDLFKIFLELLGAREKGVRVTALVPEVPGELARLTH
                     AIASNDGNIIALTQYQGTDSTNRQVMIKVTGMSKGKLLEVLKPVVISVEDARES"
     gene            complement(14013..14723)
                     /locus_tag="AC162G_14280"
     CDS             complement(14013..14723)
                     /locus_tag="AC162G_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012941226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077021.1"
                     /translation="MALLEIRDLNSGYGEVQILWSASLQLEPGHLTTLIGSNGAGKTT
                     LLRSVMGLIRPWSGSVTFEDRDVRGLSAHAKADLGLVLVPEGRQLFTDMTVYENLEMG
                     AISKRAKPHFKRNLEWVFATFPRLKERQKQNAGTLSGGEQQMLAVARGMMAQPRILMF
                     DELSLGLSPVLTISLFETLRQLKAEGLTMLLVEQNVHMALAVSDYAYVLGEGRTLIHG
                     EAGDVAKNEHVRRAYLGI"
     gene            complement(14741..15493)
                     /locus_tag="AC162G_14285"
     CDS             complement(14741..15493)
                     /locus_tag="AC162G_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012941225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077022.1"
                     /translation="MTAPILQVNNVSKRFGGLQALFDINFELPEGQILGLIGPNGAGK
                     TTLFNAINGVYPPDSGRVTFRGRDITGLPPYNVARLGLARTHQIVRPLNELTVRENVM
                     AGACFGRMDHGLSDAAREADQVLALVGLAPRAGQLAASLNVAQKKRLELARALAAQPY
                     LLLLDEVLAGLNSTEIAEMVQTIRAIRDQGITIFMIEHVMHAVMNVSDRVIVLDYGEQ
                     IAEGTPAEVAANPKVIEAYLGDPKLAERLNVA"
     gene            complement(15490..16458)
                     /locus_tag="AC162G_14290"
     CDS             complement(15490..16458)
                     /locus_tag="AC162G_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5077023.1"
                     /translation="MNLLRQPGFWLTVLLVLAGGLLAPITQSATVRESMLLVLMFVAL
                     ASSLNIILGYTGYVSFGHIVFFGLGGYVGFYFLSQQGWSLAPAALMGGLAAALLALLL
                     GLSILRLRGAYFALATIGINEAMRALITNLDIFGGPTGMSLHFNVYQEYGGATQALWI
                     TYFAMFGLALVVVVLSFAVKKSKFGLGLVSIREDEDAAMVIGVRAPRAKTWSFVLSAV
                     IPGMIGTLFFFKNGNIEPGDAFRLHMSIETIVMVMLGGQGTLLGPVLGAAGYERLRGF
                     LLTSPTFKDLQLAFAGLALLIIVLFVPAGVVGWLRIRLPSLRRVLE"
     gene            complement(16455..17318)
                     /locus_tag="AC162G_14295"
     CDS             complement(16455..17318)
                     /locus_tag="AC162G_14295"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5077024.1"
                     /translation="MLEQLIPSLVDGLLIGFVYGVAAMGLTLIWGVMRVINLAHGPII
                     ALGMFGVYFLFNDGTGLNPYLSLPIIGAAGVLFGIVIYSIAVHRVINAPHLSSLLATF
                     SVSMIIIGVGTSRLTTNVYTVGLGLGSVTIGPITVTVTRLVAAVVSMLVAAGLYLFLY
                     RTSTGKAIRAVANNRSAAELMGIPSSRVLALSFGLGTMLAVIAGGLISTIFPFRILSG
                     GAYELKSFVIGVLGGLGNPLGALVGGLILGGLEGVIPVFIETTWVPVFEFGLFVLILL
                     VRPSGLFGARQ"
     gene            complement(17480..18826)
                     /locus_tag="AC162G_14300"
     CDS             complement(17480..18826)
                     /locus_tag="AC162G_14300"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5077025.1"
                     /translation="MTRKFHLVTGLLVLAALVLSACGGAATTAPPVPTQPPAQPTQPS
                     EEPPPAPPATEPEMLTVGFTASQTGNLNVESTRQMNGLQLWMNQVNEAGGIQLSDGSV
                     LKFDATFYDDESNKDRVQELYTRLSTEDNADFLISPYSSGLADAAAVIAEQNGKIMIT
                     TGAASDSTYQKGYTLVFQAYTPASRYLTGAADLLKNLDSSVKKIAVVHENDKFSTDVA
                     NALNEYALANGYEVVLFEGYDSGATDFAPFINKIVAAEPDAIMGGGHFADTSTFARQL
                     YEKGVDAKFISLLVAPPEPKFAELGDAALGVIGPSQWEPLAQYTPEAAQAAGLEWFGT
                     GVQEFVDAYEAAYNEEPSYHSAGGYAAGLILQKAIEDADSFDTEKVKAALEAFDLLTF
                     FGHIKFDTSPENHGLQIGHDMVYIQWQTDADGNLAKQVVWPSEGKSAEALYPLPPR"
     gene            complement(19161..19850)
                     /locus_tag="AC162G_14305"
     CDS             complement(19161..19850)
                     /locus_tag="AC162G_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5077026.1"
                     /translation="MPQPTILVVDDEQPIRDFIARNLDVRGYRVALAADGLEALSVLD
                     RESPDLIILDVMMPNLNGFDVCRRIRQTSTVPIIMLTALGEESDKVTAFDLGADDYLT
                     KPFGVGELLGRLKAVLRRTRWESQPPSGEAVRFGDLEFDPERRRVARGGQEIRLTPTE
                     FTLLAELIAHAGKVLTHRALLQKVWGPEYGDEAEYLRVYVNRLRHKIEVDPSKPRHLL
                     TEQSVGYRFEP"
     gene            complement(19855..22539)
                     /locus_tag="AC162G_14310"
     CDS             complement(19855..22539)
                     /locus_tag="AC162G_14310"
                     /inference="COORDINATES: protein motif:HMM:NF014763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5077027.1"
                     /translation="MKRLNFWRRDLGVQLLALYLLFVLPVVVAALIFDAAASAQLERD
                     VKAADLALGRAIALETDAFLQQAMSAVSEFAAMPEVRSADAAQFAPLFVTAMAARPDI
                     NLFYFLDDKGTMVYHAPVGPGSTVGQDFSFRQYFKDARVTDGPVVSTGRISPTTGRPV
                     TTVVHRVLNERGQFAGVVATNLALERLSETLAEVAVQDGSAGSVLIISIIDAGGQVIA
                     HPDPRQLLSDAYTGLPRLKTLRGALPASEIAADSSGREWLYSYAPISGADWIVAVQRP
                     TEVAFASPRAFHNGLLLAMGVFVAGGLFFWMMLSRRVIGPLEQLAEFSRTVGRRDDRP
                     RSAYRAPDLTHTLARADQMGHLTRALKRMEESIERRFTELATLLETSRATSSSLDADE
                     VIDTILEQVQRLMEVNTCALVALDEREKVLRIRASRGLSEDYGRDLRIDPHDPRWPSM
                     RAIASGRPVQVSDIEADPSFEPFRDRARREGYRSLLAVPLHAPHIGPAALIVYRRDPH
                     LFGHDEVELIWNFANHAAIALENAALYRRTDEQLQEQTRVLESVMQSMNDGLILHDPG
                     GRILFVNRRFAEAMDATPADLEGRPLDQIRREMLARVIDPVAFEASLSAALDGRGPRS
                     FEFSVLRSGRRRDLRAHVFDVIDEDGNPIGHGELFQDITRYREVDRMKSALISTASHE
                     LRTPLASIKGYASTLLQDDVEWDAISVRQFVQVISDEADRLTQLVDDLLDMSRIEAGT
                     LRLHRTEQRLPDVIARALAQVKDPASHTITTDVPPDLPPLRLDARRIEVVVRNLVENA
                     VKYSPPGSAVRIRAERRDGHVQVTVSDEGPGVAPEHRDRIFDRFYRADDGYTRASGGA
                     GLGLAICKGFVEAHGGSIRLEDSEIGASFTFMLPMAVG"
     gene            22807..23631
                     /locus_tag="AC162G_14315"
     CDS             22807..23631
                     /locus_tag="AC162G_14315"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR5077028.1"
                     /translation="MTVHVYALADAGVILTDLTGPTLDAVSLHLPDGVYTTLRTYDRT
                     RILGLSAHLQRLSDSLTLLHGGRSCDPAAVRAALRIVIERENLPAARLRITAPYDNDR
                     VFISIEPFEPFPPECYSRGVRCGTTRRLARDTPRAKSSEFIKPSRSEKAGHDSDAQEL
                     LLVDAEGCIFEGISSNLFAVLDGALRTAGEGVLEGVTRGVVLALAVELLPVVCTAIHL
                     ADLPRVSEAFITSSSREVMPVVQIDQTTIGTGKPGPITQILMDRYRAYLMEAAESP"
     gene            complement(24377..>25689)
                     /locus_tag="AC162G_14320"
     CDS             complement(24377..>25689)
                     /locus_tag="AC162G_14320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5077029.1"
                     /translation="GSGLTSFALAVWIFQKTGQATPFALTVLLASLPRILLLPVAGAL
                     ADRWNRRWVMILSDAGNALVTLAVVVLLSFGDLEVWHIYLIALIGSVFSAFQEPAYTA
                     SVTLLVPKKDLARASGMMQMAQAAEMLVSPVLAGVLFVAIGLRGIVIIDFVTFFFAVG
                     ALLLVRIPQPEASSPDSRERKSVWNDAVYGWRYLRARSGLFGLLLYYALVNFLLNFSG
                     VLAGPLVLSFGTPDALGVVQTASGIGMLIGSIVMSAWGGPKRRINAVIGFIGLSSLGL
                     LLIGLQPSVVLIGVGFLLMLFCIPLASGPSQAIFQSKVALDVQGRVFAIRAMISRSMM
                     PIAYLLAGPLADQVFGPILSEGGALASTPVGALLGVGPGRGIGLMFVLSALTLAVATV
                     FAYASPRIRRVEDELPDAVPETLASAETAAAEPSGAESLPTVAV"
BASE COUNT         4716 a         8085 c         8239 g         4649 t
ORIGIN      
        1 aatagcgtga atcgtggctt tctcgccgga agaaacgctc ggtctcttgc gcacagcggt
       61 gtgccaggcc ggtcagggtg agccgtcgaa catctgatga tgtgggcatg taagaatgcc
      121 ggactcagcc gttcatatta tgtcgacgtg cggtaagtaa ggaaccccat cccacttcat
      181 ggaagagcag ttcgtgcctc ccctacacgg gcttttctct ctcaaggctg tatggaagtt
      241 gtgctgaatt gcggactgta tttgtcagta tagcattaat tggaggaaga tcaagaggtc
      301 cttgaaagac gaacgacgaa ggtctttcgt cctccgtcgt tcgtcctgtg ttcgtggccg
      361 tatatccgca ggcgcgtgta caggaggccc ggcttagccg ccagcgcctt ccgtctggtt
      421 ggtcaggtaa ttctccaggc ccatctgctc gatctgcgcc cgctgcttct cggcccaatc
      481 cacgtgccct tcttccatct tgacgatttt ggtcagcaga tcgacagtgg cctgatcgtc
      541 gacctcgtgc gccaggcgaa tcgcgctgtt atacgcgtgc acggccccca gctcggcttc
      601 ctcatcattg cccaccatct ccggcacgat cttaccgatc ttcatcgggt tgagccgtgt
      661 gacaaccggc atgccttcga ggaagatgat gcgctgaatg agccattcgg cgtggtgcat
      721 ctcgtcgcgc gcctgcccct cgatggcttt gtggaggctg tcgtagcccc agttgtcgca
      781 catctccgag tgcaccatgt actggttgat ggcggtcagt tcgtccgcca gcagttggtt
      841 caaaacctcg atgatcttgt cgtttccttt catgattgac ctccttagtc agatacttga
      901 ttcagacctg gcaggactct gctgggcgct cttgcagcac agccgggaaa agcaaacctg
      961 ccaggcctaa gacctactct ggcctgaaat agcggatgtc gagtatcgag ccggttcgtt
     1021 ccgccttcgg tttgagggcg gccgtcaccc gcatgccgac gcggggagct tgcgcgctga
     1081 attcgccgag gtcgtggacg agtgtgccgt cagcgacgtc gagccggacc aggccgagcg
     1141 tccggggcgc gtcgagcggc ttgccgtcga gatcgacgtg gacgacggtg aaactgacca
     1201 gcgtcccgcc cagaccgacc ttcacccacg cgctttcggg gagtgcggcg aagcagcgct
     1261 cgcagtacat gcgggcaggg acgtaggtgt agcggcattt gtcgcagcgc gctccaagaa
     1321 taacgccctc gtcgcgcagc gcggcgagga atcgctgccc ggcgactccg gccgtgtaac
     1381 gatagtgtgt cggcaggctg cccggcatcg agtgggcatc gccggcgtgt tcgagtgtgt
     1441 cgaggattgc catgtcggtc tccagtgatc agttatcagt gatcagtaat cagtgatcag
     1501 tgaccagtga gcagtaacca gcgagcagtg atcggtgcta ctgataactg atcactgatt
     1561 actggcaact tgctttacag cggtttccaa tgcagaatgt ccgtcaccgc accctcgcgc
     1621 tgctccggcg gcttccacac ggcctgtacc ttcatgccga cgtgcacagc tttcggatcg
     1681 acctcgccca ggaggtgcat gatacccatg ccgggcgacg ccccgtcaat atcgatcacc
     1741 gccggaatct gcggccggtc gagcttgatc atgtcccacg tcacgtagca cagcgagaag
     1801 gtcctgaccg tcccggtgtc gctcgcctca aaccactcgt cggtcgggcg gaagcactgc
     1861 tcgcagaaca tgcgcggggg gatcatccgc ctgcggcatt tgcggcagac gcgccccagg
     1921 agtttgcctt ctttcaggcc ctgcaggtat ccgccgatgg cgacgccctc gtcccaggca
     1981 tattcagcgt gaggagtcca gtcgacggtg acgacgcggt tctcatcaag atcgtcctgg
     2041 gagagactgg tcgtcgggaa gacgtgagtt tcagcagaca tgatttcact ccgttaggaa
     2101 ctgaaggaac tcagggagtc ctaccttccc tcagttccct cggttccctg agttcctatt
     2161 tccccagaac cacaactgtt ccgacctgca tcaaatctcc ccacgcctgt gcgacgccac
     2221 ggcgcggctt gccgggaacc tgccgggcgc cggcctcctc gcgcagttgc caaaacagct
     2281 cggcgatttt catcagcccg gcggcggcga tcgggttccc gacgcccagc agcccgcccg
     2341 acgggcagac gggcaagtcg ccgtcccgct gagtgacgcc gagcgccgtc gcctcggggg
     2401 ctttgccttt gtcgaagagc aacagccctt ccaggtggtg cagttccttg tagtcgaacg
     2461 ggtcgtatgg ctcggcgatg tggatctgct tgcgcggctc ggtgatgccg gccatcttgt
     2521 aggccatgcg cgccgcctct tcgacgtagg tcgggtaaga cagatcgcgg ttcgtccagt
     2581 aagccgtgtc gaggttccag cccacgccct cgatccacac cggcttgttc gtcacgcggc
     2641 gcgccacgtt gtcggcggcg agcacgacgg cgacggcgcc atcgcttacc gggctgacgt
     2701 ccagtcgctg caccggccag gccagcactt ctgagctgag gatatcttca acggtgatgt
     2761 ccgcatcgcc gagcaaggcg gccgggtggt cggcggcgtt gcgcttattc ttcaccgcga
     2821 cccgcgcaat gtcctctttc ttcagcccgt agcgctgcat gtaggcgttc atttcgagcg
     2881 cgaaaatcca cagcaggttc gggccgagag gtcgctcggt gaaattgtcg aagatggaaa
     2941 taaaggcggc ctgcgggtgc ggccggcagg gcgacatctt ctcttcgcac acgaccaggc
     3001 aggtgtcgaa caggcccgag gcgacgtgat accagccctg cgcgaccgcg aacacacctg
     3061 tgccgccgcc gacgtacccg cgcatgtaag gtttgcccca cgcgcctgcg ccgtccgaca
     3121 gccattcgct tttcatatgc acgccgtcga aggcgtcggg cgcggtgccc atcacgacgg
     3181 attcgacgtc gtccagcgtc atctcgcacg attcgagcgc cttgctcgcc gcctcccacg
     3241 ccagctcgcg cccggtctcc agcgagcggc gcacgaattg cgtcatgccc gcgcccacga
     3301 tcgcgactct gcgcatgccc atgttgacct cctgtcagtg atcagtggtc agtgatcagt
     3361 aatcagtgat cagtaagcag tgatcagtta tcacggccac cgacgaaata ctcggttaca
     3421 ggttcacgta ttgccgcaaa ggatgttcca cagaactggt cggccttcgc catcatgccg
     3481 ccaagcaaac ggccgacttc cgcgcatttg tccacgagat cgccagcctg actccgagtc
     3541 aggtaatcgc agtcggctgc cgtttcgatc caatgttgtg tttcttgctg ctcaccatcg
     3601 gcgtcggtca gtttggcgac gaagtgcgct tcgtaacgcc tcttcgccca cgcctctgcg
     3661 atttgtgcgc cgacagcacg cgacgacctc cgaatctgat tggtcagcga gtacatttcc
     3721 tccctgggaa agtctttggt cactcgaaag atctccgtgg ccagttgccg tgccttctga
     3781 tacaccacga gatcacggaa gctcttcgca tggcgtaatt cgctcatcgt gctctccgtt
     3841 gcgtccagta atcagtgaca actgacaact gataactgac aactgataac tgataactgg
     3901 tcactgatta ctgaaaactg ccgcggcgcc gctcgtcgtc ggcagtccac gccaggcgaa
     3961 ggccaggccg gtgaccgctt tgggcagttg ccgcgcgccc gcctcgcccc gcaattgaag
     4021 gacgacttcc agcgcgcggg ccaggccggt cgcgtcgagc agcgcgccga cgcccaggct
     4081 gccgcccgaa ggattgactg ggaactcgcc cgagcgctgc gtcccgccct cggccgtcca
     4141 cgcgcccgcc tcaccctcgc gcgccagccg cagcgcttcg aggtggacaa gctccttgtg
     4201 cgcgaaggtg tcgtcgattt cggcgaagtc gatctcactg cgcggcgagc gaatgccggc
     4261 ggtgcgatag gccatgtcgc cggccttgcg cgcgtagcgc gcctcgcccc acgcgcgcga
     4321 atcgagcgcg ggcgtgtcgt tggcccagcc cagcccgcgg atccagaccg gcttgccccg
     4381 cagcccgcgc gccgtgtctt cgtcggccag taccatcacg acgcacgcgt cggcgtgcgg
     4441 cgcggccatc agtcgcgtca ctggctcggc caggggcgcc gcgtccagca cgtcgcgcag
     4501 cttcacgttg gctgcatacg aggccgccgg gttggcgagc gcgttgcgct tgttcttcac
     4561 cgcgacggcg gccgcctgcg gcagcgtgaa gcgcccggcc gacagcagcc actgcatctc
     4621 cagcccggcg acgaacgacg ggtgcgccgc gagcgggcgc tccaccaccg ggtcgagcgc
     4681 gaaggcgctg atgtgcgata ggctgagaat attcgagacc ttcgagtgtg cctcgaccac
     4741 caccacgttc atcaatcccg atgcgatctg catggttgcg gcggccagcg cgtgcagccc
     4801 gtcgccggcg atcgtatgca tggggcgcag ggccgcgccg agctgatcgg gtgtgtattc
     4861 gtcgaagatc gacgtgcctt cgtgatagtc ttcggcagcc gtgacgaacc cctgcacgtc
     4921 gcggcgcggg tctatgcccg cgtcggcgta ggcgcgcgcc gccgcctcga aggccagctc
     4981 cttgtacgaa agctcgggcg tgatggggcg aaacccactc catccgatgc ccaccacggc
     5041 gacttttctg ctcatgggac ctcctgggtt gggtacatcg ctgagccaat atgggccatc
     5101 gacgggatac cggacgcgat gtgaagattg tagtttcgaa aagggtggat agtaagtgat
     5161 acgtgatacg tgaatcacgt atcactcgtc acgtgtcacg atctacgttc accacaagta
     5221 ttgtaccacc gaccgttaca gtgtcgccat tgaccagctt gcgcccgcgc cgcctctcga
     5281 cctggccgtt cacgcgtacc cggccttctt gaatcaactg cttggcctgg ccgcctgtgt
     5341 cgacgatgcc ctgccatttg aggaactggt cgagcttgat gaatttgcct gactcggtca
     5401 tcgttgtgcc atggtgcgtg gggcgcgagg cgtggagcgt ggagtgtgag tcgtggagcg
     5461 agatcacgct tcacgtctta cgcttcacgt ttcacgtccc ggccggcgag tgcttccaac
     5521 agtgccgggg gtatctcagc cggcttctgc gatgcgagat cgatccacac ccattgcgtc
     5581 tgcgcgcgaa tgtcgagtgt gccgtcctgg tcgcgcatga cggtgtgcct gacgacgctc
     5641 ggtggcgacg tctcgccaac ccaggtgagg atgtccagcg tgtcgccgta cttagccggg
     5701 tgcaggtatt cgatgtcgtg ctggcggacg acgaacatgc cgcccagatc gagccaccgg
     5761 ggcaggtcaa aaccggcgct ggcggcagcg tcgatcgtcg cctgctcgag atagttgagg
     5821 tacaccgcgt tgttgacgtg ctggttctcg tccatgtcgt agtagttgac acgccgagtc
     5881 acgtgaaacg cgttgggagg aggctcgtcc ggtgggtggg gcaggtcggc gatgatttcg
     5941 gcagccggat ccccgccgtc tggcaagaag atcgcaggca cctcgtcggg tatgcgcctc
     6001 ggcgcgccgg tacgctcgtc gacgaatacc cagtcagtcg ccgcacgcac gatcaggagg
     6061 ttgtcgccga cgcgtgtaat atcataattg cgttgcgagc ggacgcggcg gaagttgact
     6121 acgtatgtca ttacgtcaag ttcatcgccg tagcgtgcgg gttggaaata agtgatgcgc
     6181 gagcgccgga tcagccagcc aacgccgtgc tcgatgtacc actccggcgt atagccggcg
     6241 tcgctcgagg cctcgatggc agcctcttgc atgtagcgca cgtacacggc gtgattgacc
     6301 tggccgatgg cgtcacagtc tgtcgagcgc acgcggaacc ggcgggtgtg gacgcgaggc
     6361 atggcacatg gatcataggg tcgcgccgcg agaaagtcaa atcgtgcgcc gtgcctctga
     6421 gtcagcctcc ggcgagcgtg acaaaccaca gacccggcgg gtgagatctg gtacttgctt
     6481 cctaccggcg atgtggtaaa atccagccca tatcccccag gcgctgctat ctcggtctgt
     6541 gctgcttcgt gtccggttcg acctagggcc gttctctggc gaagttgcgg aagacatgag
     6601 tcaatctccc aaactcctac tggctgcttt gctgctcagc ctggcgctgg tctcgcccgc
     6661 cgtgcagccg gtggccgcgg acacgacctc atcactttcc ccttactggg gccagacgat
     6721 cgcacgctgg gaagacctga tagcccatta cgccggccag cgcgggcttg atcccgacct
     6781 ggtggcggct attatttttg aggagtccca cggcctgacc aaccggatca gcgtcgtcgg
     6841 cgcggtgggc ttgatgcaga tcatgcccaa ggagacgggg ttctcctggc gcccgacggc
     6901 agtggagctg ctcgatcccg gggtcaacct gtactggggc acgcggacgt tcgcccaggt
     6961 tgtgcaacaa gcggagggcg gcttgagccg cgccctggca gcgtacaacg gcggctggga
     7021 gcaggagcac ctgcgcgcga cgcagctctt cgccgggaaa gtgctcgacc actacgcgcg
     7081 agcgatcgcc gcgcgcgccg gcttcaatgc ccgttctatg aagaagtgga gcctggtgct
     7141 cgacattcag acgagcgcgg gcctgaagcg catccagacg attcggtcgg acgggacgat
     7201 cgatctcgac gccgaatacg acctggcgaa gctcccggcc tcgacgccgc acgcgatgac
     7261 ttattcggct atcgatgcag gcggcgcggc gtggctcgtc gaagcgtggg tcatcgtgga
     7321 gcctatcgaa ggcggcacgc ccaacgggcg tggtacttat tgacagaatt ccagacccat
     7381 ccttgcgaaa gtttgataac ctttgcaagg gtttagaaaa tgcagccact cacgggctgc
     7441 gtttttgttt tgcctcctca cgtcgaccgg ggcgaatgcg cccgggtgtg tccgtagcgc
     7501 cgcatgatat ggacggtttc ctcgatcggc aacccgtggc gcacgtggtt gtcacgcccg
     7561 accatcgtcg gcgacttgaa catcgagttg agaatggctt cctcggtcgc ttcgatcgcg
     7621 gcgatgaaga atgggctgat cggcggcgga tcgtcggggc gctcgcgcgt gaccatctcg
     7681 gccagcgcgt gccactcgta gacggatgaa tctggccggt gggcgacccg gttggcgttg
     7741 ctgaaggcga tgacgaagtc gccgctggtc gtgccgccca tcgacccgac gcgggccagc
     7801 ccgaatgacg cgcgcacggc cacgcggcgc agctcgcgca cggtgagcgg cgcatcggtg
     7861 gcgacgacga tgatgataga gttagagtgc gagttagagt ggggcgcagc acccttcgac
     7921 tccacctcgt tccgctcagg atgctgcgcg atgtgctgcc ggtccgcttc gtccagccac
     7981 gtcagcaact cgcggccgac gggcacgccg tcgatgcgca ggtcgccgcg ccgtccaaag
     8041 ttcgactgaa cgagcacgcc gaccgtaaat ctccgatctc caatccccac aatacgtgac
     8101 gccgttccaa tcccaccttt aaaccccagg cacgacatgc ccgtgccgcc gccgatcgag
     8161 ccttcctcga ccggcccggc cgttgcgccg tcgatcgcgg caaagacgtg ctcgcgctgc
     8221 acgtgccgcc cgcgaatgtc gttgagatac atgtcgctgg tctcggcgac gaccgggctg
     8281 atgccccagt cggtgatgcc catctgcgga taatgctcga acgcccagtc gatggcgtag
     8341 tcggcgacgc gcggcacgtt cagggtgttg gtgatcagga tcgggccttc gacgacgccc
     8401 atctcgtgga cctgctcgga gttcgttact tcgccgaatc cgttgatcgt gtgcacgcag
     8461 gcggcgacct tctcgtagaa cagatcgccg gaatgcggcc agacggcggt gacgccggtg
     8521 cgaatcgggc cgctgccggg tatcagtgcg ccttcgccct cgatgagggt gctgtgcccg
     8581 actttgacgc cgggcacgtc ggtgatggcg ttgtgcgtcc cggtggggag tgcgccgatg
     8641 aggatgcagc aatcgcgggt gcggggtcgc atagatcgct ccggtgttag agttagagtt
     8701 agagttagag ttgggagtta gagcagctca cgtatcacgc atcacgtatt gttgatcacc
     8761 tccgctccta gcagatcttc catcgtctcg cgcctgcgaa tcagcgaggc gctcgcgccg
     8821 tcgaccaaca cctccgccgg ccgcaggcgc gagttgtagt tggaggccat cgacatgcca
     8881 taggcgccgg cgtgcgtgat gacgagcaga tcaccgcgct gcatgggagg caatggccgg
     8941 tcgtgggcga aagcatccgc cgattcgcac accggcccga cgacatcgct caagacgccg
     9001 gtcgcgctgt gtgcccggag cgggatgatc gggtgatagg catcgtacag cgccggccga
     9061 atcagatcgg tcatgccggc gtcgaccacg acgacctgcc gcccggcgcg ccgcttcacg
     9121 tatagcacgc gagctagcaa gagtcccgcg tcggcgatga tagagcgtcc cggctcgatc
     9181 atcaactcca ggtcccgatc gcgcagcaga ggcgcaagcg cttccgcgaa gtcagatggc
     9241 gcgggaacca accccgcgtc tgcgcctgcc tgatcattca ccgggaagcc gcctccgatg
     9301 tcgagcatgc gcaggcgata gcggtcgacg agttccagcg ccttgcgcac ggcggcgacg
     9361 gtcgcatcgg gcgacgcgag ctgcgagccg acgtggacgt gtacccctgc cagatcgaga
     9421 tgcgggtagc gcgccgcatc ggcgagcagg cggccggctt cgtcgaagtc gattccgaac
     9481 ttcgagcgct tcccgcctgt cgcaatgtgt cgatgcgtat cggcctcgac ggcggggttc
     9541 aggcgcacgg cgacgtgtgg gcgagccacc atcgacgcgg cgactgcctc caacacgtcc
     9601 acttcctcgc gcgcttcgac gttgatccag cccacgcccg tttcgactgc ataccgcagt
     9661 tcggcatcgg acttgccgac tcccgcgaag accacggcgc cgggcgacgc accggcctgc
     9721 aggctgcggt ataattctcc ggcggagacg acgtcgaaac cggcgcccca ttcgcgcagc
     9781 agcctcagga tcgctccatt gctgttggct ttgacggagt atcgcaggcg tgcccggagt
     9841 ggcgcgaatg cgcgagcgac gcgctcggtg tttgccttca ggcgccgccg cgagtaaacg
     9901 tagagcggcg tgccgaagcg agcagccagg tcagccagct tcatctcttc acaggtggcg
     9961 cgcccggaat gcgtttggag catcttaact cctgccatcg gaaactatgc gattttatac
    10021 cagggtgcgg ggcggggcaa aggatcggcc cgcgcccggg cgcgggcagc ctatcaaaga
    10081 aaccagattg tccctgaagt gtaatggttt tgcattgaca tatcttgtaa aatgactaca
    10141 cttcatgcct acataagtcc ggcgaggcag tgaccggctc agcttggcgc tgctaccggt
    10201 gcgctcgccg atgaggaaat gctatggtcg ccacaatcct gatcgctgag gacgaacgag
    10261 acattcgtga cctggttgta ttcagcctgc gtttcggcgg gttcgaggtg gtggaagcta
    10321 ctaacggtca ggaagcggtg gaaaaggcgc agcaggtcat gcccgacctg atcttgatgg
    10381 acgtgcgcat gcccaggatg accggctacg acgcgtgcaa acaactcaag tcgatggagg
    10441 acgtgaagca cattccggtg atcttcctgt cggcgaaggg ccaggaacag gagattcaac
    10501 agggcatgga ggcgggtgcc gaggagtata tcctgaagcc ttttgcgcct gaagacctgg
    10561 tcaacatggt gcgcaaagtg ctgcagaaaa ctggcgcggg aagcccgtga ctttccgggc
    10621 gggtgggcgg ttgcggcgtg agcgtcatca ttatcgacaa cggcctggtg cactacgagg
    10681 cgatcggccg gggtcggccc gtcatttttc ttcatggttg gctggggtcg tggcgctact
    10741 ggatgccgac gatggaagcg atctccgatc gccatcgcac ctacgcattc gatctgtggg
    10801 gattcggcga ctcggatcac agtccgcagc gatacgatct cggagcatac gagcagcagt
    10861 tagggcagtt catcgagaat atggggttgg gcgagacgtc ggtcagcctc gtcggccacg
    10921 cgctgggtgc ggcgatcgcg gtgcgctacg ccaatcgggt cagaagcccc aatcagcccg
    10981 tgttgcgcat catggcggtc agcctgccgt taggtccgca tgccattgac cggcggctgg
    11041 cgccgggtgg cggatcgctg ctcgatcgtg tgctgggccg ccgtggcgcg gccgattatc
    11101 cggaagtcga cctggaggcg cagaaagccg acccgaacgc gatcatgatg agcgcacggc
    11161 tcctcgccac tactgaccag gaaacggcgc gcgccatgga gcggctcgat atacctctgc
    11221 tgctggttca cggcgagaaa gatccgctca tagcgctgcc ttcgcccgac tcgttgcaag
    11281 tgtcagacca gaacgtcaag ccgatcacgc tgtccgactc caggcacttt cccatgctgg
    11341 acgaagcgcc caagttccag cgcttgctca aggattttct cgaaacgagg ccggaagacc
    11401 tgcagacact ggcgctgaaa gaggagtggc ggcgccgcac ctactgatcg agactcgcca
    11461 gagcgtttct gtcttgacag tgtttgccct acgcggtaaa ctttgcaata tcattagtgg
    11521 acttgtgtaa aaggccggtg agttgaagga gcggagcatg gcgaagccga gtgtggtgac
    11581 ggatatgacg ttcgatggcg aagtgctgaa gtcgcacgtg ccggtgctgg tcgacttttg
    11641 ggcggtttgg tgcgggccgt gccggatgat cgcgccgatc gtcgacgaca tcgccaacga
    11701 gtacgacggc aagatcaaag tcatgaagct ggacgtcgac gaaaaccaga cgacggcgat
    11761 gagctacggc gtgatgggta ttcccacgct gatcctgttc aaaggcggca aggccgtcga
    11821 gcggatcacg ggttacatgc cgaaagagcg cctgctgtcc aggctgctgc cgcacctgga
    11881 cgcgacgcct tctgtgaact gacaggcgca cacaatccga aacagacaac gacggggaac
    11941 gggacgagta cacgccgcat gccgcgcaga gagtcgggga taggtggaag cccgacgcgg
    12001 acgacggcgc cgaatggacc cgggagtcgc gcaggtgaac ggtcgccgat ccagtagcct
    12061 gcgccggagc cgccaccgtt atcaaggctg agggtatcgt gacgcgaggc agacttcgga
    12121 agtctccggt ctagagcaca ctgtacccgc gagagtgagg ctggcacagg catcatccgc
    12181 cgtcacgggc ggattttgtt ttgcgccaga cctaaccagg gtggcaccac gggcatcgaa
    12241 ggacgctcgt ccctacacgg gacgggcgtt cgcgctttaa gaacaaagcg gagggttgaa
    12301 aatgaacaag ccacgcattc tcaccggcga ccggccgacc ggtccgctgc atctgggtca
    12361 ctacgtcggc acgctggtca accgcgtgcg gctgcaagac tcgcacgagt tgtttctcgt
    12421 cgtcgccgac tatcacaccc tgacgacgca gcccacgcgg gagaaggtcg cgcgcacgcg
    12481 cgagcacgcg cgcgcgatcg tgctcgacta tctgagcgtc ggcatcgatc ccgagcgcgc
    12541 gacgatctac gtgcagtccg acgtgcccga agtggccgag ttgacgctgc tcttctcgat
    12601 gctcgtcacc gtcccgcgcc tggagcgcgt gcccacgctg aaggaagtga tgcgcgagca
    12661 gaacatcacg cacccgtcaa tggggctgct gagttacccc gtgctgcagg ccgccgacat
    12721 cctcctggtg cacgccgacc tggtgcctgt cggcggcgat caggaatcgc acgtcgaggt
    12781 gacgcgcgag atcgcgcggc gattcaacca actgtacggc gcggtgttca aggagccgca
    12841 ggcgctgatc ggcgacgtgc cgatgctggt cggcatcgac ggcaagctga agatgagcaa
    12901 gagtctgggc aacgccattt acctgtccga cgacgccgag accgtcagcg cgaaggtcat
    12961 gcagatgtac accgacccgg ctcgcatcca cgccgacgtt cccggcgcgg tcgagggcaa
    13021 tcccgtgttc actttccacg atacgttcaa cgaggaccac gcggaggtgg aaaacctgaa
    13081 ggcgcgctat cgcagcggca aggtaggcga cgtggaggtg aagcagaaac tggcgcgcgc
    13141 gctcaaccga ttcctcgatc cgatgcgcgc ccgccgcgcc gggtacggag agcggccgtc
    13201 gctcgtagac gatattctgg aggcaggcgc gagccgtacc cgccctatcg ctcgggagac
    13261 gctgcggaag gcgcgcgagg cgatggggat atcgtaagta tggggtttgc acgaggatac
    13321 tacgattcgc gcgcgtcctc gacgctgatc acgactggct tcagcacctc cagaagcttg
    13381 cctttggaca tgccggtgac cttgatcatc acctgccggt tggtcgaatc ggtgccctgg
    13441 tactgcgtca gggcgatgat gttgccgtcg ttcgaggcga tggcgtgcgt gagccgggcg
    13501 agctcgccgg gaacttcggg caccagcgcc gtcacgcgca cgcccttctc gcgcgcgccc
    13561 agcagctcga ggaagatctt gaacaggtcg gtctcggtga taatgccgac cacagtcgcg
    13621 tccttcacca ccggcagccc gccgatcttg ttgtcggcca tgatgcgcgc cgcgtcctcg
    13681 agcggcgtgt cctctgtgac ggtgatgact tttttcgtca tcacgtcctc gatcttgatc
    13741 ttgccgagca ggtacgtcac ttcccacacg ctcaggctgg tggccggcga gggcgacgcg
    13801 tgcagcaggt ccttgtccga aacgatgccg atcaggtggc cgttcttgtc gacgaccggc
    13861 aggcggcgga tgcgctcctt cttcatcagg ttgagcgctt cgctgatggg gagatcgggc
    13921 gaggccagga tgggctggcg ggtcatgcgt tctttgacaa gcatacgctc ctccaaaaca
    13981 agatgtgggc taatcgcgat ggatcggcac cattatatac caagataggc tctgcgcacg
    14041 tgctcgttct tcgccacgtc gcctgcctcg ccgtggatga gcgtcctccc ctcgccgagc
    14101 acgtaggcgt aatcgctgac ggcgagcgcc atatgcacgt tctgctcgac gagcagcatc
    14161 gtcagccctt ccgctttcaa ctgccgcagc gtctcgaaca ggctgatcgt cagcaccggc
    14221 gacaggccga gcgaaagctc gtcgaacatc aggatgcgcg gctgggccat catgccgcgc
    14281 gccacggcca gcatctgctg ctcgccgccg ctcagtgtcc cggcgttttg cttctggcgt
    14341 tccttcaggc gggggaaggt cgcgaacacc cactccaggt tacgcttgaa gtgcggcttg
    14401 gcgcgcttgc tgatcgcgcc catttccagg ttctcgtaca cggtcatgtc ggtgaacaac
    14461 tggcgtcctt ccggcaccag gacgaggccc agatcggcct tggcgtgcgc cgacaagccg
    14521 cgcacgtcgc gatcttcgaa cgtgaccgag ccgctccacg ggcggatcag ccccatcacg
    14581 ctgcggagca gcgtcgtctt gccggcgccg ttcgatccga tcagcgtggt gaggtgtccc
    14641 ggctcgagct gaagcgacgc gctccacagg atctggactt cgccatatcc ggaattgaga
    14701 tcgcggattt caagcagcgc catatcttct cctctgcgtc ttacgcgaca ttcagccgtt
    14761 cggccagttt cggatcgccc agataggcct cgatgacttt aggattggcc gccacctccg
    14821 ccggcgtgcc ctctgcgatc tgctctccat agtcgagcac gatcacccga tccgacacgt
    14881 tcatcaccgc gtgcatgacg tgctcgatca tgaagatagt gatgccctga tcgcggatgg
    14941 cgcggatggt ctgcaccatt tcggcgatct cggtagagtt aagcccggcc aggacctcgt
    15001 cgagcaacag caagtaaggc tgagcggcaa gcgcgcgggc gagctccagt cgctttttct
    15061 gggcgacatt caggctggcg gcgagctgcc cggcgcgcgg cgccagccct accagcgcca
    15121 gaacctgatc ggcctcgcgc gccgcgtccg acaggccgtg gtccatgcgc ccgaagcacg
    15181 cgccggccat cacgttctcg cgcacggtca gctcgttgag cgggcggacg atctgatgcg
    15241 tgcgcgccag gccgagccgc gccacattat acggtggcag gccggtgatg tcccgccccc
    15301 ggaaggtcac gcgcccgctg tccggtgggt atacgccgtt gatggcgttg aataacgtgg
    15361 tcttgcccgc gccattcggc ccaatcaggc cgaggatttg accttccggc agctcgaagt
    15421 tgatgtcgaa cagcgcctgt agaccaccga agcgcttgct cacattgttg acctgaagga
    15481 tcggtgcggt cattccaaca ccctccgcag gctaggcaat cggatccgca gccagcccac
    15541 aactccggcc gggacaaaca ggacgatgat gagcagcgcc agcccggcaa aggcaagctg
    15601 cagatccttg aacgtcgggc tagtgagcag gaatccgcgc aggcgctcat aaccggccgc
    15661 gccgagcacc ggcccgagca gcgtgccctg cccgcccagc atcaccatga cgatagtctc
    15721 gatcgacatg tgcaggcgga aagcgtcgcc cggctcgatg ttgccgttct tgaagaaaaa
    15781 cagggtgccg atcatgcccg ggatgacggc ggagagcacg aacgaccacg ttttggcgcg
    15841 cggcgcgcgt acgcctatga ccatggccgc gtcttcgtct tcccgaatcg ataccaaacc
    15901 cagcccgaac ttcgacttct tgacggcgaa gctcagcacg accacgacca gtgccaggcc
    15961 gaacatcgcg aagtacgtga tccacaacgc ctgtgtcgcg ccgccgtact cctgatacac
    16021 gttgaagtgg agcgacatgc cggtcggccc gccgaagata tccagattgg tgatcagagc
    16081 gcgcatggcc tcgttgatgc cgatcgtcgc cagcgcgaag tatgcgccgc ggagccgcaa
    16141 gatcgacagg cctagcagaa gcgccagcag cgcggccgcc agcccgccca tcagcgccgc
    16201 cggcgccagc gaccaaccct gctggctcag gaagtagaag cccacgtagc cgcccagccc
    16261 gaagaacacg atatggccga agctcacgta gcccgtgtaa cccaggatga tgttcaggct
    16321 ggaagccagc gccacgaaca tcagcaccag aagcatgctt tcgcgcacgg tagccgactg
    16381 cgtgatcggc gcgagcagcc cgccggccag cacgagcagc acggtcagcc aaaatccggg
    16441 ttggcgcaag aggttcattg gcgtgctccg aacaacccac tgggacggac gagcagaatc
    16501 aagacgaaca ggccgaattc gaacactggc acccacgtag tctcaatgaa caccgggatc
    16561 acgccttcca gcccgccgag aatcagcccg ccgaccagtg cgccgagcgg gttgcccagc
    16621 ccacccagca cgccgatgac gaagctcttc aactcgtagg cgccgcctga caggattctg
    16681 aagggaaaga tcgtggagat cagcccgccg gcgatgacgg ccagcatggt gcccagtccg
    16741 aagctcaggg ccagtacccg gctcgacgga atgcccatca gctcggcggc gctgcggttg
    16801 ttggcgacgg cgcgaatcgc cttgcctgtg gaagtgcgat acagaaagag atacagcccg
    16861 gccgctacga gcatcgacac gacggcggcc accaggcgcg tcaccgtcac ggtgatcggg
    16921 ccgatcgtca cgctccccag gcccaggccg acggtgtaca cgttggtcgt gagccgtgag
    16981 gtgccgacgc cgatgatgat catgctcacg gagaaggtgg ccagcagcga cgagagatgc
    17041 ggcgcgttga tcacgcggtg tacggcgatc gagtagatca cgatgccgaa caacaccccg
    17101 gccgccccga taatcggcag gctcaggtac gggttcaatc ccgtgccgtc gttgaacagg
    17161 aagtacacgc cgaacatgcc gagggcgatg atcggcccgt gcgccaggtt gatcacgcgc
    17221 atcacacccc agatcagggt gagacccatc gcggccacgc cgtatacgaa accgatcagc
    17281 agtccgtcga cgagcgaggg aatcagttgc tcaagcatgg aggctctccg tatgcagcag
    17341 atggcggata gccaatggcc gatagctgat ggccgatggc tcgtcggcgt cggaatgatc
    17401 acatatcgcc gtagtggcgc agcacacctt ccggcgatat gcgataagcg atacgctatt
    17461 ggctatccgc catcagcgat cagcgtggcg ggagcggata cagtgcttcg gcggacttgc
    17521 cctcgctcgg ccacacgacc tgcttggcca ggttgccgtc ggcgtccgtc tgccactgga
    17581 tgtacaccat gtcgtggccg atctgcaggc cgtggttctc ggggctggtg tcgaatttga
    17641 tgtggccgaa gaaggtgagc aggtcgaatg cctccagcgc ggccttcacc ttctcggtgt
    17701 cgaacgagtc ggcgtcctcg atcgcctttt gcaggatcag gccggcggcg tagccgcctg
    17761 cgctgtggta ggacggctcc tcgttgtagg ccgcctcgta cgcgtcgacg aactcctgga
    17821 caccggtgcc gaaccactcc agcccggcgg cctgggccgc ttccggcgtg tactgcgcca
    17881 gcggctccca ctggctgggg ccgatcacgc cgagcgccgc gtcgcccagc tcggcgaact
    17941 tcggttcggg cggggcgacg agcagcgaga tgaacttggc gtccacgcct ttctcgtaca
    18001 gctgcctggc gaaagtgctg gtgtctgcaa agtggccgcc gcccatgatc gcatccggct
    18061 cggcggcgac gatcttgttg atgaacgggg cgaagtcggt cgcgccgctg tcgtagcctt
    18121 cgaacagcac tacctcgtag ccgttcgcca gcgcgtactc gttgagcgcg tttgccacgt
    18181 cggtcgagaa cttgtcgttc tcgtggacga cggcgatctt cttgaccgac gagtcgaggt
    18241 tcttcagcag gtcggccgcg ccggtcaggt agcggctggc gggcgtgtag gcctggaaga
    18301 cgagcgtgta gcctttctgg taggtggagt cggaagccgc gccggtcgtg atcatgatct
    18361 tgccgttttg ctcggcgatc accgccgccg cgtccgccag tccgctcgaa tacgggctga
    18421 ttaggaagtc ggcgttgtcc tcggtgctca gccgggtgta gagctcctgc acgcggtcct
    18481 tgttgctctc gtcgtcgtag aatgtggcgt cgaatttgag cacgctgccg tcgctcaact
    18541 ggatgccacc ggcctcgttg acctgattca tccacagttg caaaccgttc atctggcgcg
    18601 tcgactcgac gttgagattg ccggtttgcg acgcggtgaa tccaacggtg agcatctccg
    18661 gctcggtggc gggtggagcc ggcggcggct cttctgacgg ctgggtcggt tgggcgggcg
    18721 gctgggtcgg aaccggcggc gctgtcgtcg ccgcgccgcc gcaggcggaa aggacaaggg
    18781 cggccagcac aagcaggcca gtcacgagat ggaacttgcg ggtcatcttt tcctcctcaa
    18841 tgtgaaaggg atgcaacaca caggatgcgg atacaagagg gcaagaagag gatcaagcca
    18901 tcgcctcctt tcagcaagtg atgtatcgac gggcgactat caccacttta ctgccgaggt
    18961 cgataacggc gcataaacgc gcggaggcga gatataaaaa atgtgtaaat gcaactgctc
    19021 agcctcaccc cccaaccccc tctcccgaag tgaagaggcg cacttcggga gagggggagc
    19081 gccaactccc ctctcctgac gacagccgtt gcgtcgtcag gagaggggtc gggggtgagg
    19141 cagcctgcgg ggagcaggcg ctagggttcg aagcgatacc ctacactctg ttcggtgagg
    19201 agatgccggg gtttcgaggg atcgacttcg atcttgtggc gcagccgatt gacgtagact
    19261 cgcaggtatt cggcctcgtc gccgtattca ggtccccata ctttctggag cagcgcgcgg
    19321 tgggtgagga cttttccggc gtgcgcgatc aactcggcca gcagtgtaaa ctcggtcggg
    19381 gtgagccgga tctcctggcc gccgcgcgcc acgcgccgcc gctccggatc gaactccagg
    19441 tcgccgaagc gaacggcttc gcccgacggc ggctggctct cccagcgtgt ccggcgcagc
    19501 acggctttca ggcggcccaa tagctcgccg acgccgaacg gcttggtgag gtagtcgtcg
    19561 gcgccgagat cgaatgccgt caccttgtct gattcctcgc cgagcgcggt gagcatgatg
    19621 atggggacgg tcgacgtctg ccggatacgc cggcagacgt cgaagccgtt caggttgggc
    19681 atcatcacgt cgaggatgat cagatcgggc gactcgcggt cgaggacgga cagcgcttcg
    19741 agtccatcgg ctgccagcgc gacccggtag ccccgcacgt ccaggttgcg agcgatgaaa
    19801 tcgcgaatgg gctgctcgtc gtcgacgacg aggatcgtgg gttggggcaa aggcttatcc
    19861 caccgccatc ggcagcatga acgtgaaact cgcgccgatc tcgctgtcct ccagccggat
    19921 cgacccgccg tgcgcttcga cgaagccctt acagatcgcc aagcccagcc cggccccacc
    19981 gctggcgcgg gtgtagccgt cgtcggcgcg gtagaagcga tcaaagatgc gatcgcgatg
    20041 ctccggcgcg acgccggggc cttcgtcact taccgtaacc tgcacgtgtc catctcggcg
    20101 ctcggcccga atgcgcaccg ccgagccggg cggcgagtat ttgacagcgt tctcgaccag
    20161 gttgcggacc acgacctcga tgcgcctcgc gtcgagacgc agcggcggaa ggtcgggcgg
    20221 cacatccgtg gtgatcgtgt gactggccgg gtccttcacc tgcgcgagcg cgcgggcgat
    20281 gacatccggc aggcgctgct cggttcgatg gagccgcagg gtgccggctt cgatccggct
    20341 catgtccagc aggtcgtcga ccaattgcgt cagtcgatcg gcctcgtcgc tgatcacctg
    20401 cacgaactgg cggacggaga tggcatccca ctccacgtcg tcttgcagca gcgtgctcgc
    20461 atatcccttg atcgacgcga gcggcgtgcg cagctcgtgc gaggcggtgc tgatcagcgc
    20521 cgacttcatg cgatcgactt cgcggtaacg tgtgatgtcc tggaaaagct cgccgtgccc
    20581 gatcgggttg ccgtcctcgt cgatcacgtc gaacacgtgc gcacgcaggt cgcggcggcg
    20641 accgctgcgt aggacgctga attcgaagga tcgcgggcct cggccgtcga gcgccgccga
    20701 gagcgacgct tcgaacgcga ccggatcgat cacccgcgcg agcatctcgc ggcgtatctg
    20761 gtcgagcggc cggccctcca gatcggcggg cgtggcgtcc atcgcctcgg cgaagcggcg
    20821 gttgacgaac aggatgcgcc ctcccggatc gtgcaggatc agtccgtcgt tcatgctctg
    20881 catgaccgat tctagaacgc gcgtctgctc ctgcaactgc tcgtcggttc gcctgtacag
    20941 tgcggcgttc tccagcgcga tcgcggcgtg attggcaaag ttccagatca gctcgacctc
    21001 gtcgtgaccg aagaggtgcg gatcgcgccg gtagacgatg agcgccgccg ggccaatgtg
    21061 cggcgcgtga agcggcacgg ccagcaggga gcgatagccc tcgcgccgcg cccgatcccg
    21121 aaacggctca aatgacggat cggcctcgat gtctgaaacc tgcaccggcc ggccggacgc
    21181 gatggcgcgc atggacggcc agcgcggatc gtgcgggtcg atgcgcaagt cgcgcccgta
    21241 atcttcgctc aggccgcggc tggcgcggat acgcagcacc ttctcgcgct cgtcgagcgc
    21301 caccagcgcg cacgtgttca cttccatcag gcgctgtacc tgctccagga tcgtatcgat
    21361 gacctcgtcc gcgtcgaggc tggacgacgt ggcgcggctc gtttccagca gcgtggccag
    21421 ctcggtgaag cgccgctcga tcgattcttc catgcgcttg agcgcgcgcg tcaggtggcc
    21481 catctgatcg gcgcgcgcca gcgtgtgcgt gaggtcgggt gcacggtaag ccgagcgcgg
    21541 gcgatcgtcg cgccggccca ccgtccggct gaactcggcg agctgctcca gcggcccgat
    21601 cacccgccgt gacagcatca tccagaagaa caagccgccg gcgacgaaca cgcccatcgc
    21661 gagcaacagc ccgttgtgga aggcgcgcgg cgaggcgaag gctacctcgg tcgggcgctg
    21721 cacggcgacg atccagtcgg cgcccgaaat cggcgcgtag gagtacagcc attcgcggcc
    21781 cgacgaatcg gcggcgatct cgctggccgg aagagcgccg cgcagcgttt tgagtctcgg
    21841 cagcccggtg taggcgtcgc tcagcagttg ccgcgggtcg ggatgcgcga tgacctgccc
    21901 gcccgcgtcg atgatgctga tgatcagaac ggagccggcg ctcccgtcct gaaccgcgac
    21961 ttcggcgagc gtctcgctca agcgctccag cgccagattc gtggcgacca cgccggcaaa
    22021 ctggccgcgc tcgttcagaa cgcgatgcac aaccgtcgtg accggccggc cggtggtggg
    22081 agagatgcgc cccgtcgaga cgacggggcc gtcggtcacg cgggcatcct tgaaatactg
    22141 gcgaaacgag aagtcctggc ccaccgtgct gcccggtccg accggcgcgt ggtacaccat
    22201 cgtgcctttg tcgtcgagga agtaaaacag gttgatgtcg gggcgggcgg ccatcgccgt
    22261 cacgaataga ggagcgaact gggcagcgtc agccgaccgc acttcgggca tggccgcaaa
    22321 ctcgctcacg gccgacatcg cctgctgcaa gaaagcgtcg gtctcgagcg cgatcgcgcg
    22381 cccaagcgcc aggtcggcgg ctttcacgtc acgctcgagc tgcgcgctgg ctgcggcgtc
    22441 gaagatcagc gcggcgacga cgacgggcag gacgaacagc agatacagcg ccaggagttg
    22501 aacgcccaga tcgcgtcgcc agaagttcag ccgtttcatg ctgtgcagta actgctccgg
    22561 tcgccttttt tcttccacga attcacacga atcttcacga aggaatgttc aacttcgtgt
    22621 cgcttcgtgt ggcttcgtgg acaactccga agcgggcgag tagttacgcc gtgcagaaca
    22681 aaggaaagtg ccaggccggg aaagactggt agcagtatag gcgaatctgc gccgattgtc
    22741 cataccgtgc tacatgggtg ccaatgcgaa ttctccgccg cgtggtagcg cgttatactt
    22801 gtgccgatga ccgtacacgt gtatgcgctc gccgatgccg gcgtgattct gaccgatctg
    22861 accggaccta cgctcgacgc cgtctctctg catttgcccg acggcgtcta cacgacgctc
    22921 cgcacgtacg atcgaacgcg cattctggga ctgagcgccc acttgcagcg gctgagcgac
    22981 tcgctgaccc tgcttcatgg cgggcggtct tgcgatccgg cggcggttcg cgctgcgctg
    23041 cgaatcgtga tcgagcggga gaacctgccc gccgcgcgcc tgcgcatcac cgcgccgtat
    23101 gacaatgacc gcgtgttcat cagtatcgaa ccctttgagc cttttccgcc ggaatgctat
    23161 tcgcggggcg ttcgctgcgg cacgacgaga cggttggcgc gggatactcc ccgagcgaaa
    23221 agctccgagt tcatcaagcc ctcgcgcagc gagaaggcag gacacgactc ggatgcccag
    23281 gagctgctgc tggtggacgc agaaggctgc atctttgagg gcatctccag caacctcttt
    23341 gccgtgctgg acggcgcgct gcgcacggct ggagaggggg tactggaagg cgtgacgcgg
    23401 ggagtcgtgc tcgcactggc ggtagaacta ctgccggtcg tctgtacggc gattcacctc
    23461 gccgatcttc cgcgtgtgtc ggaggcgttc atcaccagct ccagccggga agtgatgccc
    23521 gtcgtgcaga tcgaccagac gaccatcggc acaggcaagc cgggaccgat cacccaaatc
    23581 ctgatggacc gctaccgcgc ctacctcatg gaagccgccg aatcgccctg acgggctgta
    23641 tccagcaggg tgaagtcgaa tcgggcgtga agattaatca agatatggtc ggtactcggg
    23701 ccggatgtgt tctggtgagc gatcgggaac cgggaaggca ggggcagcgt ctgtatatag
    23761 tgccaggcga gcatgaagcc cgcttgtgca cacctggagg taagcagaca caggaggaca
    23821 tcatgccacg aagaggattc ttcgcgacgc tgtttgtggt tgctctgatc ggcgcagcct
    23881 gcgcgccagc ggtcatcccg cccgcgccga cgccgactgt cgagccgacg ccatcgatcg
    23941 agccggcaca acccggcagc ggcaaagtga tctccggtct ggccacggtc gagagtatca
    24001 cgctcatcat gctggagtcc ttccccgtcc aggtcagggt gaccgcaacc ggctacctgc
    24061 ccgacccgtg cacgaaaatc agcggcacgg agcagacgat ggaaggggac accatctatc
    24121 tgaagatcat gaccagccgt caggtcgacg tgatgtgcat tcaagtgctg gcgccgttcg
    24181 aggagactta cccgctcgac gtggccggcc tgcccgccgg gacgtacacg gtggacgtca
    24241 acggtgtgac cgacacgttc acgctcgacg tcgacaacgt gctgccgccg gaagccacgc
    24301 acaggacgct atcttggacg gaggcagaga agatgatagg gaacaggatc cagggtgtca
    24361 ttcgagactc agagaatcac accgccactg taggcagcga ctctgcgcct gacggttctg
    24421 ctgccgccgt ctcggcgctt gccaacgttt cgggcaccgc gtccggcagt tcatcctcga
    24481 cgcgtcgaat gcgcggactg gcgtaggcga acacagtggc cactgccagt gtcaacgccg
    24541 ataacacgaa catcagcccg atgccccggc ccggtccgac gcctagtaga gcgccgaccg
    24601 gcgtgctcgc cagcgcgccc ccttcgctca gtatcgggcc gaatacctga tcggccagtg
    24661 gcccagccag aagataggca atgggcatca tcgagcgcga aatcatggcg cggatggcga
    24721 acacgcggcc ctgcacgtcg agcgccacct tggactgaaa aatcgcttgg ctggggcccg
    24781 aggccagtgg gatgcagaac agcatgagca agaagcccac gccgatcagg acgacgctcg
    24841 gctgcaggcc gatgagcagc aggccgagcg acgacaaccc gatgaaaccg atcacggcgt
    24901 tgatgcggcg cttcggcccg ccccacgcgc tcatcacaat actgccgatc agcattccga
    24961 tccctgaggc cgtctgcacg acgcccagcg cgtcgggtgt gccgaaagag agcaccagcg
    25021 gaccagccag cacgccggag aaattcagca ggaagttcac cagggcgtag tacaacagca
    25081 ggccgaacaa cccgctccgg gcgcgtagat agcgccagcc atacaccgcg tcgttccaga
    25141 ccgacttgcg ctcacggcta tccggcgacg acgcttccgg ctggggaatg cgcacgagca
    25201 gcaacgcccc gacggcgaag aagaaagtga cgaagtcgat gatcacgatg ccgcgcagac
    25261 cgatcgccac gaacaacacg ccggccagca ccggcgacac gagcatctcg gctgcctggg
    25321 ccatctgcat catcccactg gcgcgcgcca ggtccttttt cggaacgagc agagtgaccg
    25381 atgcagtgta ggccggttcc tgaaaggccg aaaacaccga tccgatcagc gcaatgagat
    25441 agatatgcca cacctcgaga tcgccgaagg acagcagaac gacgacggcc agggtgacca
    25501 gagcgttgcc agcgtcggac aggatcatga cccaccgacg attccagcgg tcggccaggg
    25561 cgccggcgac tggcagcaac agaatgcgcg gcaggctggc gagcagcacc gtcaacgcaa
    25621 agggcgtcgc ctggcccgtc ttctgaaaga tccacacggc aagcgcgaaa ctggtcaggc
    25681 cggagccga
//
LOCUS       DBNHXB010000350         3199 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3618734, whole genome shotgun sequence.
ACCESSION   DBNHXB010000350 DBNHXB010000000
VERSION     DBNHXB010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3618734"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="AC162G_14325"
     CDS             complement(<1..456)
                     /locus_tag="AC162G_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077030.1"
                     /translation="MKRLMLVLVVLALLIAPSTAMADGSGGCATIKDGTIMDSAGNPI
                     TTGYDQFGYDYQSHMFNGTYDGVDRIWDGKYWGQTGDFVDDKLMMKWSDAWLANVDCN
                     GDGKLDRGLVDGIPDPYEISKGWLTNHVEGDYYDGAGGLQSYTYFVKIVW"
     gene            complement(905..1639)
                     /gene="atpF"
                     /locus_tag="AC162G_14330"
     CDS             complement(905..1639)
                     /gene="atpF"
                     /locus_tag="AC162G_14330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR5077031.1"
                     /translation="MEGLPLGIVPGIVLAQLVNFVILFLILRVVAFKPLLNVLQQRKE
                     RIAKGLEDARRAEEKLANIEKDYQAKLDEARAEGQKAIAEASATADRQAAAIVAKANE
                     DAARLKAQAQEEAELERNRILADLRSQVASLAIAATNKLIGASLDEQRQRALVDEFFS
                     GVRSGRVVVVEAGSLPAKVQVTSALPLSESEQAAIKVDLSGHGASEVEFHVDPKILGG
                     LVIRAGERVVDASVAGQLESLRQSLV"
     gene            complement(1662..1901)
                     /gene="atpE"
                     /locus_tag="AC162G_14335"
     CDS             complement(1662..1901)
                     /gene="atpE"
                     /locus_tag="AC162G_14335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR5077032.1"
                     /translation="MATATALAKYLGAGFAMIGAIGAGIGIGLSVGGAVQAMGRNPDA
                     APLIQTNMILGVAFAEAIGIFCLVVSLILLFVVQA"
     gene            complement(2036..>3199)
                     /locus_tag="AC162G_14340"
     CDS             complement(2036..>3199)
                     /locus_tag="AC162G_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR5077033.1"
                     /translation="TRVVIIAVVMFLCGCTALFNLPGLPQLFPVVLPTISVEPEPVAC
                     IGGGLPHCEGGFPITNSLVATLIVDLLLIAVFFSAARRPKLVPSSVQNFLESLIEILY
                     NQAEQVAGRNARKIFPVGATIFLFVFFANYMELLPGVDSVGYVHRVEEEGVQGYELDR
                     STPEGAPIVFLNPACPVIAQDRYDRLTDAQKTAREEAGCREPIQVAEAAGGSHAAEGE
                     HEVDRGWTVVPTVRTASTDINVTLALGLVAFAATQVYGVMGHIPHGRKGAAAVLTGIK
                     RYLSKFFNVGGFRRPGPGKIFGAIDFFAGLLEGLSEFVRVVAFTFRLFGNIFAGTVLI
                     FVVMYLVPYFGPVAALLLETFVGLIQAYVFMMLTFVFMHAAAQVHGEHGEAAH"
BASE COUNT          628 a         1039 c          949 g          583 t
ORIGIN      
        1 ccatacgatc ttgacgaaat atgtgtagct ctgcaggccg cccgctccgt cgtagtaatc
       61 gccctcgacg tggttggtca accaaccctt gcttatctca tagggatcgg ggatgccgtc
      121 gacaaggcca cgatccagct tgccatcgcc gttgcagtcc acgttggcga gccaggcatc
      181 gctccacttc atcatgagct tatcatcgac gaaatcaccc gtctgacccc agtacttgcc
      241 gtcccagatt cgatcgacgc cgtcgtaggt gccattgaac atgtgcgact ggtagtcgta
      301 tccaaactgg tcataacccg tggtgatcgg attgcctgca ctgtccatta tggtgccgtc
      361 tttgatggtc gcgcatccac ctgacccatc ggccatcgca gtcgatggtg cgatcaataa
      421 tgcgagcact accaacacaa gcatgagccg tttcattgtg cttcctcctg tgcgtcttga
      481 gacacagtca cccatgaaag ggagacacga gtcgaactac tctgaaggat cgaggtcgcc
      541 gagcctgcgg cgagcagcga acgatactgc acttatgatc tctatatgca tcatctccct
      601 ctgcgtccgt tcagcatacg tgttcattgt aggactgaat tccaccacgc gtcaatacgg
      661 tcacgcgtaa ttctgacgca actctaacga caaaagactc cacccgtaac gatcgaacca
      721 cgttcgacgc cgcgagtggt tggcatcccg taataagaaa tacgaccaca aataaaaaaa
      781 acgccgagac caaaattgat ctcaacgttt cgaacataga cctggcaggt cttgttcgcc
      841 gatgtcagcc gcgggctaca ccacgaagac ctgccaggtc taccctgcgt ctagtataac
      901 gtgatcagac caggctctgc ctcagcgatt ccaactgtcc tgccaccgat gcatccacga
      961 cgcgttcgcc ggcgcggatc accaggccgc ccaggatctt cgggtcgacg tggaactcca
     1021 cttccgacgc cccgtgcccg ctcagatcga ccttgatagc cgcctgctcg ctctcgctca
     1081 agggcagcgc gctggtgacc tggaccttgg ccggcaggct gcccgcttcg acgaccacga
     1141 cccggcctga tctgacgccg ctgaagaact cgtcgacgag cgcgcgctgg cgctgctcgt
     1201 cgagcgaggc gccgatcagc ttgttggtcg cggcaatggc gagcgacgcc acctgcgagc
     1261 gcaggtcggc caggatgcgg ttgcgctcca gctcggcctc ttcctgcgcc tgggccttca
     1321 ggcgagccgc gtcttcgttg gccttggcga cgatggcggc cgcctgcctg tccgccgtgg
     1381 ccgacgcctc ggcgatggcc ttctggccct cggcgcgcgc ctcgtcgagc ttggcctgat
     1441 agtccttctc gatgttggcg agcttctctt cggctctgcg cgcgtcttcc aggcccttgg
     1501 cgatgcgctc cttgcgctgc tgcagcacgt tgagcagcgg cttgaacgcg accacgcgca
     1561 ggatcaaaaa caggataacg aagttcacaa gctgtgccag gacaatgccg ggcacaattc
     1621 ccaagggtaa cccttccacg ccaggcctcc tcttatctcg actatgcctg aacgacgaac
     1681 aataggatca gagagaccac caggcagaaa ataccgatgg cttcggcgaa ggccacgccc
     1741 aggatcatgt tcgtctggat gagcggtgcg gcgtccgggt tgcggcccat ggcctgcact
     1801 gcgccgccga ccgacaggcc aatgccgatt cctgcgccga tcgcgccgat catggcgaag
     1861 cccgcaccga gatatttagc cagagccgtt gcggttgcga tatccacgtc gtctcctcct
     1921 gaaagagtta gagtgatgag ttagagtagg ggcgacccct gcgatgcgct ccgagcgaag
     1981 cgagcgagtc gcggccgccc gatggggcag gggcaagccc tgaccttacg attgtctaat
     2041 gagcggcctc gccgtgttcg ccgtgcactt gcgcggccgc gtgcatgaac acgaacgtga
     2101 gcatcatgaa cacgtaggct tgaatcaggc cgacgaaggt ctccagcagc agcgcggcga
     2161 ccggcccgaa gtacggcacc aggtacatca cgacgaagat caacaccgtg ccggcgaaga
     2221 tgttgccgaa caaccggaaa gtgaacgcga cgacgcgcac gaactcgctc aggccttcca
     2281 gcaggccggc gaagaagtcg atcgcgccga aaatcttgcc cggccccggg cgccggaagc
     2341 cgccgacgtt gaagaacttg ctcaggtagc gcttgatgcc ggtgagcacg gcggccgcac
     2401 ctttgcggcc gtgcgggatg tggcccatca cgccgtacac ctgcgtcgcg gcgaaggcga
     2461 ccagcccgag cgccagcgtg acgttgatgt cggtcgacgc ggtgcgcacc gtgggcacca
     2521 cggtccagcc ccggtcgacc tcgtgctcgc cctcggccgc gtgcgagcct cccgccgcct
     2581 cggcgacctg gatcggctca cggcagcccg cctcctcgcg cgccgtcttc tgggcgtcgg
     2641 tcagccggtc atacctgtct tgcgcgatga ccgggcaggc cgggttaagg aacacgatcg
     2701 gcgcgccctc gggcgtcgat cggtcgagct cgtagccctg cacgccctct tcctcgacgc
     2761 gatgcacgta gccgacgctg tcgacgccgg gcagcagttc catgtagttg gcgaaaaaga
     2821 cgaagaggaa gatcgtcgcg ccgacgggga aaatcttgcg ggcgttcctg ccggctacct
     2881 gctccgcctg gttgtacaga atctcgatca gcgattcgag gaaattctgc acgcttgacg
     2941 gcaccagctt cggccggcga gccgcgctga agaacaccgc gatgagcagc aggtcgacga
     3001 tcagcgtggc gacgagcgag ttcgtgatcg ggaaaccgcc ttcgcagtgg ggcagcccgc
     3061 cgccgatgca cgccaccggc tcgggttcga cgctgatcgt tggcagcacg accgggaaca
     3121 attggggcag ccccgggaga ttgaacagcg ccgtgcagcc gcacaggaac atcaccacgg
     3181 caatgatgac gactcgcgt
//
LOCUS       DBNHXB010000351         5906 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3786386, whole genome shotgun sequence.
ACCESSION   DBNHXB010000351 DBNHXB010000000
VERSION     DBNHXB010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3786386"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..436)
                     /locus_tag="AC162G_14345"
     CDS             complement(50..436)
                     /locus_tag="AC162G_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5077034.1"
                     /translation="MAEEKKKVVCIEDEPEMIDLVKLILGRKGFELTGAMGGREGLEA
                     VRRVKPDLVLLDLMMPDMDGWEVYQQMKADPEMQKIPVIVVTAKAQSIDKVLGLHIAK
                     VDDYVTKPFGPQELLQSVEKVLGIQV"
     gene            complement(693..1085)
                     /locus_tag="AC162G_14350"
     CDS             complement(693..1085)
                     /locus_tag="AC162G_14350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5077035.1"
                     /translation="MPGPKHRVVWIEDDPELLRLGRLVLAHKGFEVVPVNDSREGFDI
                     IRREKPDLVLLDLMMPEVDGWAIYNQMKAEPDLKDTPVIVVTAKAQAADKALALYLAK
                     VDGYLVKPYDQNQLLDMIHHVMSRRQQS"
     gene            complement(1122..2459)
                     /locus_tag="AC162G_14355"
     CDS             complement(1122..2459)
                     /locus_tag="AC162G_14355"
                     /inference="COORDINATES: protein motif:HMM:NF005913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5077036.1"
                     /translation="MIALNPSAVREEAVRHLQALLRIDTTNPPGNESAAADYLASVLR
                     AEGYEPLLLEAEPGRANLIARYPGDGSAAPLLLYSHTDVVPAETDKWTHPPFSGAVAD
                     GQIWGRGALDMKAMVAMELMTMLLLKRSGDAPRRDVIFAATADEEIDSDAGAEWLVKH
                     HPDLVRAEYGISEFGGYSLHFEGRRFYPVMTAEKGVCWMKIRARGQPGHASVPKEDNA
                     VLQLARAIDRAAGKPLPLHVTPTAAEFIRRVAAALDWPQAIGLRALLNPLTHRIAEAR
                     VGRDGVAGSLRASLHNTVSPTQLAAGTKVNVIPSEAVAALDGRLLPGFDRDSFLAELQ
                     PRLGRHIEVEVEHYSPPLEVPADTPLFGAIESGLRRYDPDGIVVPHMLTGATDAKLFS
                     QLGARCYGFSPLRLRPDEPFDRLIHAHDERISVDALTFGVQVLYETVRDFCRQ"
     gene            2760..3578
                     /locus_tag="AC162G_14360"
     CDS             2760..3578
                     /locus_tag="AC162G_14360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016426 - tRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HIR5077037.1"
                     /translation="MRLARAGDLALFISRDHKQFLTQLQPGESLHTHRGIIAFDDCIG
                     SAWGRDIRSHLGYPFVVLEPSTADLVRQIKRTTQIIFPKDVGYILMKLSIQPGITVVE
                     AGTGSGGLTLALARVVGSSGRVISYEVRPEMQNLARKNLEKAGLAGCVEFKLKDVESG
                     FDETDVDALFLDVPEPWRCLAQAASALRSGGFFGTIVPTANQVIRLLDVLRDQPFGLI
                     EVEELLLRAYKTVPERLRPMDRMVAHTGFLLFARKIDRTWQAEPETGETAEGGE"
     gene            complement(3580..4227)
                     /locus_tag="AC162G_14365"
     CDS             complement(3580..4227)
                     /locus_tag="AC162G_14365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR5077038.1"
                     /translation="MDQIVLNTTTRTVIGKQVRALRRHGQVPLVMYGAQTDAVSLQAD
                     AKELARVLRQAGGSRLIAVSTGGRMQNALARAVQREPISGQILHVDLLAVSMTERITV
                     EVPIVLDGKSPAVRRGEGVLVTGIGSIEIECLPGDLIDNIRVDLERLASVGDALYVKD
                     LNVPATVEVLTDLEEMVARVTYLAAEEVAPVEALPTEAEPEVIARGKVEEEGEEE"
     gene            complement(4275..4775)
                     /locus_tag="AC162G_14370"
     CDS             complement(4275..4775)
                     /locus_tag="AC162G_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077039.1"
                     /translation="MAEPKPVEIAGQTIDHVQGDIVRLQQCAVQQIDADQVQLVRSAA
                     ARIEAEWVEMLQGAAQVVEADTARMRNSVAFSVRAEAIAAEQSSAVALRAGEITLADS
                     NTGVLIGQNVQAGNVNAVVFIGRNVDGQVHTALDARGALLLGLAFGAALGALSLLGAL
                     LERSRR"
     gene            complement(4930..5649)
                     /locus_tag="AC162G_14375"
     CDS             complement(4930..5649)
                     /locus_tag="AC162G_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077040.1"
                     /translation="MKPRRRGFLRLLLVSLPAIALLVSGFGAFTSLVLEEDDEFCISC
                     HRPPEQAYFDRARQAQDGSTPADLSSLHMNTREMAFNCIACHRGASRIGDRISSVLLG
                     ARDTLIHFAGAGDPALEKRSAALPDLIDRSCVKCHVDAIVVAGFENHFHVKLPAAWQA
                     IQSGVAPIAPPNLPPGSPDPLADSQGKPEPLETTVNCLSCHLAHVTGFESVNYLDESA
                     TLPACDTCHIETDRGPKGLVP"
     gene            complement(5649..>5906)
                     /locus_tag="AC162G_14380"
     CDS             complement(5649..>5906)
                     /locus_tag="AC162G_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077041.1"
                     /translation="RLSYVAGVLAVIHFVWLVKSDITEPVRFGLVVALLLALRIPIVR
                     KTISGARARATGSIRSASERRQASLRARSLPPRPQRTLREP"
BASE COUNT          981 a         1913 c         1944 g         1068 t
ORIGIN      
        1 gggggggccg ccgctcatgg ggcgaaccgt gggggaggcc tcggcgtgct cagacttgga
       61 tgccaagcac cttctcgacg ctctgcagca gctcctgcgg gccgaagggc ttcgtgacgt
      121 agtcgtcgac cttggcgatg tgaaggccga gcaccttgtc aatgctctga gccttagccg
      181 tgacgacgat gaccggaatc ttctgcatct cggggtcggc cttcatctgc tggtacactt
      241 cccaaccgtc catatccggc atcatcaggt cgaggagaac gagatcgggt ttgacgcgcc
      301 gcacggcctc caggccctct cgcccgccca tcgcgccggt cagctcgaag cctttacggc
      361 ccaggatgag cttgaccagg tcgatcattt ccggctcatc ctcgatgcaa acaactttct
      421 tcttctcttc ggccatgctg attgtcgctc cttatggatg aacgagagtc cggcgcattg
      481 ccggggcgct cctgcaccag gccaggctag cggcggctaa ctccgctgag gattccggcc
      541 tgcgaattcc tacaagtcta caccaaaaga gggcaagtgt ctagtactgg tgacgacccc
      601 tgacgccccg caagtccgtt cgcccgcagt ggcactgcgg gcgccggacg acagtggaac
      661 tgtcgctcca actcgtgtgc gatcgccaat cattaggact gctgcctgcg gctcatgacg
      721 tggtggatca tgtcgagtag ttggttctga tcgtaaggct tgaccagata gccatccact
      781 ttggccaggt acagcgccag cgccttgtcg gcggcctgcg ctttggccgt cacgacgatg
      841 accggcgtgt ctttcaggtc gggttcggct ttcatctggt tataaatcgc ccagccatcc
      901 acctcgggca tcatcaggtc cagcagcacc aggtccggct tttcgcgccg gatgatgtcg
      961 aatccctcgc ggctgtcgtt gacggggacg acctcgaacc ccttgtgggc caggacgagc
     1021 cggcccagtc ggagcagctc cgggtcgtcc tcgatccaca cgacgcggtg tttggggcct
     1081 ggcatggcga tgatgactcc tcgtctgtcg gggcgctgcg cctactgccg gcagaagtcg
     1141 cgcaccgttt cgtacaagac ctgcacgccg aaggtgagcg cgtcgacgct gatccgctcg
     1201 tcgtgcgcgt ggatcaggcg gtcgaacggc tcgtcggggc gcagccggag cggcgaaaag
     1261 ccgtaacacc gtgcgccgag ctggctgaac agcttggcgt ccgtcgcgcc ggtgagcatg
     1321 tgcggcacga cgatgccgtc gggatcgtat cgccgcaggc cggactcgat cgcgccgaac
     1381 agcggcgtgt cggcgggaac ctcgagcggc ggcgagtagt gctcgacttc gacctcgatg
     1441 tgccggccca ggcgcggctg aagctcggcg agaaacgaat cgcgatcgaa gccaggcagc
     1501 agccgcccgt ccagcgccgc cacggcttcg gacgggatga cgttcacttt cgttccggcg
     1561 gccaattggg tgggcgacac agtgttgtgc aggctggcgc gcaggctgcc ggcgacgccg
     1621 tcccggccca cgcgcgcctc cgcgatgcgg tgcgtgagcg ggttcagcag cgcgcgcagg
     1681 ccgatcgctt ggggccagtc gagcgcggcc gcgacgcgcc gaatgaactc ggcggccgtc
     1741 ggcgtgacgt gcagcggcaa cggcttgccg gcggcgcggt cgatcgcgcg ggcgagctgg
     1801 agcacggcgt tgtcttcttt cggcacggac gcgtggccgg gctgcccccg agcgcgaatc
     1861 ttcatccagc acacgccttt ttcagccgtc atcacgggat agaaacgccg cccctcgaaa
     1921 tgcagcgagt atccgccgaa ctcgctgatg ccgtattcgg cgcggacgag atcggggtga
     1981 tgcttcacca gccattccgc ccccgcgtcg ctgtcgattt cctcgtcggc cgtggcggcg
     2041 aagatcacgt cgcggcgggg cgcatcgccg ctgcgcttga gaagcagcat ggtcatcagc
     2101 tccatggcga ccatggcttt catgtcgagc gcgccgcgcc cccagatctg cccgtcggcg
     2161 acggcgcccg agaagggcgg gtgggtccac ttatccgttt cagcggggac gacgtccgtg
     2221 tgcgagtaca acagcagggg cgcggccgaa ccatcgcccg ggtagcgcgc gatcaggttg
     2281 gcgcggcccg gttccgcttc cagcagcagc ggctcgtagc cctcggcgcg cagcacgctt
     2341 gccagatagt ccgctgcggc cgattcgttg ccgggcgggt tagtggtgtc gatgcgaagc
     2401 agcgcctgga gatggcgcac ggcttcttca cggacggcgg acggattaag ggcgatcatc
     2461 ggctcgacca cacgaacgcg tcaccgcaga cgccgaaggc gcgcggcggg tgcagtcaaa
     2521 tcctgacgag taagtttact ctatttatct ggagtttggc aaatcactgg agtcgagtct
     2581 tcaggcggtc atctctggcg gtcaaaaccg gttaaagcct cgactccaac ccaagatatg
     2641 acactggcca aagtccagta tttatttgag gctacaagcg gcgaagttgc aatagggcgt
     2701 tagggtcatt ataatgcgca cactcccgtg agagcaaccc gacccgaatg gagcacgcta
     2761 tgcgactagc gcgcgcaggc gacctggccc tcttcatcag ccgcgatcac aagcagttcc
     2821 tcacccagct acagcccggc gagagcctgc acacccatcg cggcatcatc gccttcgacg
     2881 attgcatcgg cagcgcgtgg gggcgggaca tccgctcgca cctgggatat cctttcgtgg
     2941 tcctggagcc gtcgacggcc gacctggtgc gccagatcaa gcgcacgacc cagatcattt
     3001 tccccaagga cgtcggctac atcctgatga agctgtcgat ccagccgggc atcaccgtcg
     3061 tcgaggccgg gaccggctcc ggcgggctga cgctggcgct ggctcgcgtc gtcggctcgt
     3121 ccgggcgcgt gatcagctac gaggtgcgcc cggagatgca gaacctcgcc cggaagaatc
     3181 tggagaaagc aggcctggcc ggctgtgtcg agttcaagtt gaaggacgtc gaatcgggct
     3241 ttgacgagac ggatgtggac gcgctgttcc ttgacgtgcc cgagccgtgg cgctgcctgg
     3301 cgcaggccgc aagcgcgctg cgcagcggcg gcttctttgg gacgatcgtc ccgaccgcga
     3361 accaggtgat tcgcctgctg gacgtgctcc gggatcagcc gtttgggctg atcgaagtcg
     3421 aagagctact gctgcgggcg tacaagaccg tgccggagcg gctgcgccca atggaccgca
     3481 tggttgcgca caccggattc ctgctgttcg cacgcaagat cgatcgcacc tggcaggcag
     3541 agccggagac gggcgagacg gctgagggtg gtgagtgagc tactcttctt cgccttcttc
     3601 ctcgaccttg ccgcgtgcga tgacctccgg ctcggcttcg gtgggcagcg cttcgacggg
     3661 cgcgacttct tcggcggcca gatacgtgac gcgcgccacc atctcttcca gatcggtaag
     3721 cacctcgaca gttgccggga cgttcagatc tttcacgtac agcgcgtcgc cgacgctcgc
     3781 cagccgttcg aggtcgacgc ggatgttgtc gatcaggtcg ccgggcaggc actcgatctc
     3841 gatcgagccg atgccggtga ccaggacgcc ctcgccgcgc cggacggcag gcgatttgcc
     3901 atcgaggacg atcggcacct cgaccgtgat ccgctccgtc atcgacacgg ccagcagatc
     3961 gacgtgcagg atctggccgc tgatcggctc gcgctgcacg gcgcgcgcca acgcgttctg
     4021 catcctgccg ccggtgctga cggcgatcag gcggctgccg ccggcctgcc gcagcacgcg
     4081 cgccagttcc ttggcgtccg cctgcagtga caccgcatcg gtctgcgcgc cgtacatgac
     4141 cagcggcacc tggccgtggc gccgcagcgc ccgcacctgc ttcccgatga cggttcgagt
     4201 tgtcgtattc agtacaattt gatccatgcg tagtaactcc ctcagaaacc aggtttctcg
     4261 aagcaacctg gtttctagcg ccgcgaccgt tccagcagcg caccgagcag cgacagcgca
     4321 cccagcgccg cgccgaaggc caaacccagg agcagcgcgc cgcgcgcgtc gagcgccgtg
     4381 tggacctgcc cgtcgacgtt gcggccaatg aagacgaccg cgttcacatt gccggcctgg
     4441 acgttctgtc cgatcagcac cccggtgttg ctatctgcga gcgtgatctc gccggcccgc
     4501 agcgcaacgg cgctgctttg ctcggcggcg atggcctccg cccggacact gaatgccacg
     4561 ctgttgcgca tgcgggcggt gtcggcctcg acgacctgcg ccgcgccctg cagcatctca
     4621 acccactcgg cttcgatgcg ggccgccgcc gatcgcacca attgcacctg gtcggcgtcg
     4681 atctgctgca ccgcgcactg ctgcaagcgc acgatgtcac cttgcacatg atcgatggtt
     4741 tgaccggcga tctcgacggg tttcggctcg gccatgttgc cctctgaata ggtcatgcca
     4801 caaaaatggg cttggccttg cgccagccca tccacgatgg taaattctac atgcgaacgc
     4861 gcgattcgtc aagatttgaa ctttgacctt tggagtttgg atttttgatt tgccctgagg
     4921 actgatcggc tatggcacca gccccttcgg cccgcgatcc gtttcgatgt ggcaggtgtc
     4981 gcacgccggc agggtggcgc tttcgtcgag gtagttgacg gactcgaagc ccgtcacgtg
     5041 cgcgaggtgg cacgacaggc agttgaccgt cgtctccagc ggctcgggct tgccctgcga
     5101 atcggcgagc ggatcgggcg atccgggcgg caggttggga ggcgcgatcg gcgcgacgcc
     5161 gctctggatc gcttgccagg ccgccggcag cttgacgtga aagtgattct cgaacccggc
     5221 gacgacaatc gcgtcgacgt ggcattttac acaactgcgg tcgatcaggt cgggcagggc
     5281 ggcgctgcgt ttctcgagcg ccggatcgcc cgccccggcg aagtggatga gcgtgtcgcg
     5341 cgcgccgagc aacacgctgg agatgcgatc gccgatccgg ctcgcgccgc gatggcaggc
     5401 gatgcagttg aaggccatct cgcgcgtgtt catgtggagg ctggacaggt cggcgggcgt
     5461 gctgccgtct tgcgcctgcc gcgcgcggtc gaagtacgcc tgctccggcg ggcgatggca
     5521 cgagatgcag aattcgtcgt cctcttcgag gacgaggctg gtgaacgcgc cgaacccgct
     5581 taccaggagt gcgatcgccg gcagcgaaac gagcagaaga cgaagaaagc cgcgccggcg
     5641 cggcttcatc aaggttccct cagtgttctt tgcggcctcg gtggtagact cctcgcgcgc
     5701 aggctggctt gcctccgctc cgacgcgctg cgtatggacc ccgtcgcgcg ggcgcgcgcg
     5761 ccgctgatcg tcttgcgcac aatcgggatg cgcagcgcca gcagcagcgc gacgacgagt
     5821 ccgaaccgga ccggctcggt gatgtcggac ttgaccagcc acacgaagtg aatgacggcc
     5881 agcacgccgg cgacgtacga caggcg
//
LOCUS       DBNHXB010000352         5651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4457217, whole genome shotgun sequence.
ACCESSION   DBNHXB010000352 DBNHXB010000000
VERSION     DBNHXB010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4457217"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2222)
                     /locus_tag="AC162G_14385"
     CDS             complement(<1..2222)
                     /locus_tag="AC162G_14385"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HIR5077042.1"
                     /translation="MTEIHGLGHSRKRVEDARFIRGQGNYIDDLKLPGMLYLDIVRSP
                     YAHARIKHIDSGKALAVPGVLAVITGETLKQYNVHNMPTLMSDTQMVLPVDTVKYQYQ
                     EVAGVLATDRYTAADGVAAVQVEYEPLPVVVDPKKALEPGAPKVRDDKDSNHIWHWEW
                     GDREAANSAFEAAEVTVKQDIYLPRIHVSSIETCGCVAKWDAAEGHLTLWMTTQAPHA
                     IRTVVALVTAHLGLSEEKIRVISPDIGGGFGGKVPVYPGYVIAVAASIVTGKPVKWIE
                     DRTENLAADSFARDYHMAAELAGKRDGTLTALRIKTIADHGYTDAAANPSKFPAGLFH
                     ICTGSYDFQSAFVEVDGVYTNKPPGGIAYRCSFRVTEAVHAIERMADLMAHEIGMDPA
                     EFRLKNFIKPEQFPYTSALGWEYDSGNYGGALRKAMHMIGYEQLRKEQAEKRARGELM
                     GIGISSFTEIVGAGPSHTFDILGLKMFDSCEIRVHPTGKAIARFGTKSQGQGHETTFA
                     QIIAEELGIPSEHIKVEEGDTDTAPYGLGTYASRSTPTAGAAAAMASRKIRDKARKIA
                     AHLLEAAEEDLVWETGKFYVRGSPDKAKTIQEIAFAAYTNHPLGMEAGLEAVNYYDPP
                     NLTYPFGSYICAVDIDRGTGEVKVRRFVAVDDCGVRINPMIVEGQIHGGLTMGFAPAL
                     MEEISYDENGNMYGSTFMDYMLPTAVETPRWETGETVTPSPHHPIGAKGVGESATVGA
                     P"
     gene            complement(2345..2821)
                     /locus_tag="AC162G_14390"
     CDS             complement(2345..2821)
                     /locus_tag="AC162G_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5077043.1"
                     /translation="MSTHHIKVTVNGEVKEADVEARTLLVHFIRENLNLTGTHIGCDT
                     TSCGACTVHWNGKPVKACTVFAVQADGGEIRTVEGLAQNGKLHPIQEGFHQMHGLQCG
                     FCTPGMMMTAVALLEQNPNPSEEDIRWGISGNLCRCTGYVNIVKAVQYAAEKIKAQ"
     gene            complement(2818..3693)
                     /locus_tag="AC162G_14395"
     CDS             complement(2818..3693)
                     /locus_tag="AC162G_14395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR5077044.1"
                     /translation="MIPPSFDYVAPKTLPEAVALLGQHADAKVLAGGHSLIPLMRFRL
                     ASPAVVVDINRIDGLSYIREEDGWLKIGAMTREAELERSALITEKYPLLSDTTRMVAD
                     PLVRNMATVGGNLAHADPANDHPAAMLAYGAQLVATGPNGQRTIPIAEFFTGPFTTAL
                     ERNEILTEIRIPAHKPGDGGCYLKVERKVGDYATAAVAVQVTLDGNGSCASAGIGLTN
                     VGLTPIRATAAEEALRSKAPNDDHIRQAARLASEAAEPESDRRGSEEYKHALVRTLTA
                     RALHKAIARARGGQR"
     gene            4300..5193
                     /locus_tag="AC162G_14400"
     CDS             4300..5193
                     /locus_tag="AC162G_14400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR5077045.1"
                     /translation="MKPPVFEYVVPESLDVALAAIAEHGGDAKPLAGGQSLVPAMNFR
                     VAQPAVLVDLNPLLELDYVRQDDDGLRIGAMTRQRRVERDALVAERAPLLHETMPYIA
                     HPQIRNRGTIGGSLAHADPAAELPVVAVALDARIKARSASGERWIPAAEFFQGIFTTA
                     LNPDELLVEVAFPAMPPRAGWAFVEFARRHGDYALMGVATVVAVDEQGVCRQARLVYL
                     NAGDGPVAAKQAVQLLLGHPPSPDSIAAAANMAVDREITPFGNVHATADYQRHLARVL
                     TRRALKQAFERAGGSISLVVR"
     gene            5262..>5651
                     /locus_tag="AC162G_14405"
     CDS             5262..>5651
                     /locus_tag="AC162G_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5077046.1"
                     /translation="MPHLHTVKVTVNGNQYERQTEARTLLSDFLRHELGLTGTHVGCE
                     HGVCGACTILFDGQPIRSCLTFAVQANGHAITTVEGLAKSQDELHPLQQAFWEAHGLQ
                     CGFCTSGILMTLVPFLEENPNPSELEIR"
BASE COUNT          966 a         1763 c         1738 g         1184 t
ORIGIN      
        1 ggcgcgccga cggtggccga ctcgccgacg cccttcgcgc cgatcgggtg gtgcggcgaa
       61 ggcgtgaccg tctcgccggt ttcccacctc ggcgtctcga cggccgtcgg cagcatgtag
      121 tccatgaacg tgctgccata catgttgccg ttttcgtcgt acgaaatctc ctccatcagc
      181 gcgggagcga agcccatcgt cagaccgccg tggatttgtc cctcgacgat catcgggttg
      241 atgcggacgc cgcaatcgtc gacggccacg aagcggcgca ctttcacctc gcccgtgccc
      301 cgatcaatgt ccactgcgca gatgtacgag ccgaaggggt aggtgagatt gggtggatcg
      361 tagtaattca cggcttcaag cccggcttcc attcccagag ggtgatttgt gtaggcggca
      421 aaggcgattt cctgaatcgt cttcgccttg tccggcgagc ctctcacgta gaacttgccg
      481 gtttcccaaa ccaagtcctc ctcggccgct tccagcaggt gggctgcgat cttccgggcc
      541 ttgtcgcgaa tcttgcggga ggccatggcg gcggcggcgc cggctgtggg cgtgctgcgg
      601 ctggcgtagg tgcccaggcc gtagggcgcc gtgtcggtat ccccttcctc gaccttgatg
      661 tgttcggaag ggatgcccag ttcttcggca atgatctggg caaaggtcgt ttcgtgtccc
      721 tggccctggg acttggtccc aaagcgagct atagccttgc cggtggggtg gacgcgaatc
      781 tcgcaggaat cgaacatctt caggccgagg atgtcgaacg tgtgcgacgg cccggcgccg
      841 acgatctcgg tgaacgacga aatgccaatg cccatcagct cgccccgcgc gcgcttttcg
      901 gcttgctcct tgcggagctg ctcgtagccg atcatatgca tcgccttgcg cagcgcgccg
      961 ccatagttcc cgctgtcgta ctcccagccc agcgcggacg tgtaggggaa ctgctccggc
     1021 ttgatgaagt tcttcagacg gaactcggcc gggtccatgc ctatctcgtg cgccatcagg
     1081 tcggccatgc gctcgatagc gtgcacggcc tcggtgacgc ggaaggagca gcggtaggcg
     1141 atgccgcccg gcggcttgtt ggtgtagacg ccgtcgacct cgacgaacgc gctctggaag
     1201 tcgtacgacc cggtgcagat gtggaacagg ccggcgggga acttggaggg gttagcggct
     1261 gcgtccgtgt agccgtgatc ggcgatcgtt ttgatgcgca gtgcggtcag cgtgccgtct
     1321 ctcttgcccg ccagttcggc ggccatgtgg taatctcggg cgaacgagtc ggcagccagg
     1381 ttctcggtgc ggtcttcgat ccacttgacc ggcttgccgg tgacgatcga tgcggcaacg
     1441 gcgatgacgt agccggggta aaccggcact ttgccgccga agccgccgcc gatgtccggc
     1501 gagatgacgc gaatcttttc ctcggacagc cccaggtggg cggtgacgag agcgaccacc
     1561 gtgcgaatgg cgtgcggcgc ctgcgtcgtc atccacagcg tcagatgccc ttctgccgca
     1621 tcccacttcg ccacgcagcc gcaggtttcg atggaagaca cgtggatgcg cgggaggtag
     1681 atgtcctgct tgacggtgac ctcggcggcc tcgaacgcac tattggccgc ttcccggtcg
     1741 ccccattccc agtgccagat gtggttgctg tctttgtcgt cgcgcacctt tggggcgccc
     1801 ggctccagcg ccttcttggg gtccacgacg accggcaggg gctcatattc gacctggacc
     1861 gcagccacgc cgtcagccgc cgtgtagcgg tcggtcgcca agacgccggc cacttcctgg
     1921 tattgatatt tcaccgtgtc gacgggaagc accatctgcg tatcgctcat cagcgtcggc
     1981 atgttgtgga cgttgtactg cttgagcgtc tctccggtga tcacggccag cacgccgggg
     2041 acagcgagcg cttttccgga gtcgatatgc ttgatccgtg cgtgagcgta tgggctgcgc
     2101 acgatgtcca ggtacaacat gcccggcagc ttgaggtcgt cgatgtagtt gccctggccg
     2161 cgaatgaaac gcgcgtcttc cacgcgtttt cgactatggc ccaaaccgtg tatttcagtc
     2221 atagtgtcct cctcgtcagt gatcagttgt cagtgatcag ttgtcagtta tcagtcatca
     2281 gttatcagtc atcagttgcc gcccaccatt cactgattac tgatcactga ttactgatta
     2341 ctggtcactg tgctttgatc ttctccgcag catactgcac cgccttgacg atgttcacgt
     2401 accccgtaca gcggcacagg ttacccgaga tgccccagcg gatatcctct tccgatggat
     2461 tcgggttctg ttcgagcagc gcgacggccg tcatcatcat gccgggtgtg caaaagccgc
     2521 actgcaggcc gtgcatctga tggaatcctt cctggatggg gtgcagcttg ccattctggg
     2581 ccaggccctc gaccgtccga atctcgccgc cgtcggcctg cacggcgaac acagtacagg
     2641 ctttcaccgg tttaccgttc cagtgcaccg tgcacgcgcc gcacgacgtc gtgtcacagc
     2701 caatgtgcgt gcccgtgaga ttcaggttct cacgaatgaa atggacgagc agcgtgcgcg
     2761 cttcgacgtc ggcttccttg acctcgccgt tgaccgtcac cttgatgtgg tgggtgctca
     2821 tcgctgacct ccccgcgctc gtgctatggc tttgtgcagc gcgcgagccg tgagcgtcct
     2881 gaccagcgcg tgcttgtact cctccgagcc acgccggtcg ctctccggct cggccgcttc
     2941 ggatgccagg cgggccgcct gccgaatgtg gtcgtcgttc ggtgctttgc tccgcagcgc
     3001 ctcctcagcc gccgtggccc tgatcggcgt caatccgacg ttcgtcaggc caatgccggc
     3061 ggacgcgcaa cttccgtttc cgtcgagtgt cacttgcacg gcgaccgccg ccgtcgcata
     3121 gtcgccgacc ttgcgctcga ccttgagata acacccgccg tcgccgggct tgtgcgccgg
     3181 gattcggatt tcggtcaata tttcattgcg ctccaatgct gtggtgaacg ggccggtgaa
     3241 gaactcggcg atggggatcg tgcgctgacc gttggggccg gtcgcgacga gctgtgcgcc
     3301 gtatgccagc atcgccgccg ggtggtcatt ggctgggtcg gcatgcgcca ggttgccgcc
     3361 gaccgtcgcc atgttgcgca cgagggggtc ggcaaccatc ctggttgtgt cggagagcag
     3421 agggtacttc tctgtgatca gcgcggagcg ctccagctcg gcttctcgcg tcatcgcgcc
     3481 gatcttcagc cagccatcct cttcacgtat gtaggacagt ccgtcaatgc ggttgatgtc
     3541 gacaacaaca gcgggcgacg ccagtctgaa tcgcattaac ggaatcaggc tgtggccgcc
     3601 cgcaagcact tttgcatctg catgctggcc cagaagcgca actgcctccg ggagcgtttt
     3661 cggcgcaacg taatcaaaag aaggtggaat catgtagagt ctcctcccca ttaccaaacg
     3721 gacatgctgg tcgtcgtatg atgtggaaga cggatacgct atctgcctgg cctgcacctc
     3781 cttgaataag tgcctatccg aaaactagcg ggttgggctc ccacgcccag acatctggca
     3841 gcatgcccac tctgctgcgc tacgggcgct tcgcaaggag ccacctccgc tgggatatcc
     3901 gggtaggctc taagattgag ttttcggtga agccgacgag cgagagaatg atgcggactg
     3961 cgaatgcacg caacaccgca ggagttgtct gcggcgaagc ccttggcgcg tgcaagtact
     4021 gcaagtccta ttatatcaaa ctcgaccgat gagcacaagg catttttgct ctggtgatga
     4081 ttcacatact gccgggcgtg ggagcctggc ctgccctctc ttccacagcc tgctcgtcgc
     4141 catcatcagg ccatttgtgt gattttcaaa acctggcgat aatatgcgac gctcggatag
     4201 gaggcatcaa tcgccgaatt ctcggcgccg gccctctatc caatcgagtg acacacatcc
     4261 ttacccccgg cgagacaatt tacggtcgag gcatggccca tgaagccacc cgtatttgaa
     4321 tacgttgtgc ctgaatcttt ggatgttgct ttagccgcga ttgcagaaca cggcggcgat
     4381 gccaagccgc tggcgggtgg gcagagcctc gttccggcca tgaacttccg cgtcgcccag
     4441 ccggctgtgc tggtcgattt gaacccgctg ctcgagttgg actacgtacg ccaggatgac
     4501 gatggtctgc gcatcggcgc aatgactcgc cagcgtcgtg tcgagcgaga tgcactggtt
     4561 gccgaacggg cgcccttgct gcacgaaacg atgccttaca tagcgcatcc acaaatacga
     4621 aaccggggga cgattggcgg cagtctcgcg cacgccgatc cggccgccga gttgcccgtc
     4681 gtcgccgtcg cgctcgacgc gcgcattaag gcgcgcagcg cgtccggcga gcgctggatt
     4741 cccgccgccg agttctttca gggcattttc acgacggcgc tgaacccgga tgagctgctc
     4801 gtcgaagtgg cgttccccgc catgccgccg cgggcaggct gggcgttcgt cgaattcgct
     4861 cgccgtcatg gcgactacgc gctgatgggc gtggccacag tcgtcgccgt cgatgaacag
     4921 ggcgtgtgtc gccaggcgcg gctcgtgtac ctgaacgcgg gcgatggccc tgtcgcagcc
     4981 aaacaggctg tgcagcttct actgggtcac ccgccttcgc ccgattcgat cgcggccgcg
     5041 gccaacatgg ccgtggaccg cgagatcacg cccttcggca acgtccacgc caccgccgac
     5101 taccagcgcc atctcgcccg ggttctgacc cggcgcgcac ttaagcaggc gttcgaacgg
     5161 gcaggagggt cgattagtct ggtagtcaga tagtcacgta atcacgtaat cacgtgatca
     5221 catgatttcg tagccattag ccctcatcgg gagaggcatc gatgccccat cttcacacag
     5281 tgaaagtcac tgtcaacggg aatcaatacg aaaggcaaac tgaggcacgc accctgttga
     5341 gcgacttcct gcgacacgag ctcggcctca ccggcacgca cgtcgggtgc gagcacggcg
     5401 tgtgcggcgc ctgcacgatt ctcttcgacg ggcagccgat tcgctcgtgc ctgaccttcg
     5461 ccgtgcaggc caatgggcat gccatcacga ccgtcgaggg cctggcgaag agccaggatg
     5521 agctacatcc cttgcagcag gcattctggg aagcccacgg cttgcagtgc ggcttctgca
     5581 cgtcgggcat tctgatgaca ctcgtgccgt ttcttgaaga gaacccgaat ccctcagagc
     5641 ttgagatccg t
//
LOCUS       DBNHXB010000353         5930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1439234, whole genome shotgun sequence.
ACCESSION   DBNHXB010000353 DBNHXB010000000
VERSION     DBNHXB010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1439234"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="AC162G_14410"
     CDS             <1..1060
                     /locus_tag="AC162G_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077047.1"
                     /translation="QEYDAWQALFAAQPTLVQRFLEAQARALADAIPQPHPVSQMRFT
                     LPDRILLEVPQAGPGQSVPIPSDQREQLAGGLLDRVTGGGADKVLRQRLAELEQSPNR
                     AVAVSAGLLRHVTVMHMVHGMLPAGRSVTYTALPDEEIPSIPVGGPLEPESAITAATD
                     AIAEEGEIESGRGELLVPYVPAARRFYLPQWVAFDDRDRLLVNSISEAEAMVASMQRF
                     VDVLHAAIALAVYMVVDDEYQRKRYGMLGQLVNQGRALARYQTGEIIRTIKARATAHD
                     LNRGLSLSLPYFDDQTLCVEPHDFVVIPGGRIMFVAALVVRAARQEQAKVAQDTRFNA
                     STRHHLLSLLEMLVRAFS"
     gene            1077..2984
                     /locus_tag="AC162G_14415"
     CDS             1077..2984
                     /locus_tag="AC162G_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5077048.1"
                     /translation="MLSVILLVVFFLIAYKLAPRENKEHAHVHGAGQIGLLAALALFG
                     VDYFTSYFYATGEMMHALHPYGLQQYAYIAVAVIGFANFVFGALYMYSLGIFNEGGGS
                     YTASMRYLWPTLSLIVAVTLIQDYVLTIVVSALSGGDQLLSILGAYGQNWLYHFGIGA
                     ALAAVTWWLTIRGRGESSRVVFTLLGIFIMMTITMAIGLFIAHAKSVPPAPVEEATHS
                     VTLAEALLHMLTASMKGMVALTGLEAMSNGIQFVINEDTGIVKWGRKHLPRLNKLWDF
                     YSGKSGIGRFVQTSFLFYGGLTTLFLTIFAIRFNVFDGTDGRTLVGNLAFIGFGQIQG
                     GDLLFWAYQILAVLLLAAASMTAFQDAQATEWRDVAIGEIPEVIVYRDPRGTFTRSVT
                     ITFGLAVLIMFLVRGQTTHAVPFYGVGVFMPITVMGLAIRRHVQQHYTGRVRKWGVFG
                     ATIATVLAGIVFLGQIVGKWSEGGWVVLISFTLLALAAHAMLLSPLGYREPKQIHRIV
                     RDKARVQGAMASIVEWQSLKMQEYRYALLVGTARFFELFGVRRPVRYEVPVVAGDYDH
                     ALHIDHPEAPSLLEQYLDARPEPHIGGPPNVTAVGDLNPLVQPNGPGESVESDRAADR
                     QIASANDGETA"
     gene            3023..5824
                     /locus_tag="AC162G_14420"
     CDS             3023..5824
                     /locus_tag="AC162G_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR5077049.1"
                     /translation="MRSAPIYSLRVSDVFRALETSPEGLASNEAQARLSLYGPNVLRE
                     PKAAPQWRRFGGHVLHPMALLLLSAGVLASLGGRPELGIVIWLIVMVNAGFSFWREYR
                     AEQAVTALKQLLPSYARIVRDGGEVQVPSSEVVPGDVLVLAEGDNIPADARVVEEYGL
                     RVNNASLTGEAVPARKTAEASLREGLTEVERPNLIFASTSVVSGTGRAVVYATGMATQ
                     FGRIANLTQVVEEPPSPLQQQLRWMTRLITFVAIGIGTVVFLVAALNVGMPRIEALIL
                     AVGIIVATVPEGLSPTVALSLAMAVQRLARRGVLVKKLATVETLSATSVICTDKSGTL
                     TQNQMTVRQVWAGGQRFSVSGAGYEPTGEFSPRPDDTVAGDFKALLGAAMLCNNARLI
                     PPSPEHPHWTCLGDQTEAALRVLALKGGMDDRLLNAMLPRVHELPFDARRKRMTTIHR
                     DSVYGEVAMVKGAPKEVLQLCTNILMHGEVRPLDAETRATIMAANDDDARRALRVIAL
                     SRRELPPRSGPYSVEDIERDLTFLGLVAMMDPPRPEVADAVKTCRQAGIRIAMITGDY
                     GLTAESVARRVGMLSTPNPRILTGTDVEALNDAELQALLGEEAVFARMAPDHKLRLVA
                     AFQARGDVVAVIGDGVNDAPALRKADIGIAMGRAGTDVAKQAADVIITDDDFGAIAGA
                     IEEGRTAYNNIRKFLTYIFASNVPEIMPFVLMALFNIPLALAVTQILAIDLGTDLLPA
                     LALGAERPEPDVMQRPPRWRTLPLVDGSLIARALWLGAIETVLCYVGFFFVIDGSLNL
                     AMTSRPDLLPFTDRLVVRGGLAYVMATTVFHAGVVTAQIGNAFACRTELSHVRHMGWL
                     SNRFLLAGIAAEIAIILSLVYVAPLAAVFEHTPLPPEYWLVLLAYAPILYVCEWIRKR
                     AVQRIEQRQLRRRQGVVLI"
     gene            5821..>5930
                     /locus_tag="AC162G_14425"
     CDS             5821..>5930
                     /locus_tag="AC162G_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019748254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR5077050.1"
                     /translation="MKVIIMGCGRVGEELCRIMLGDGHEVAIIDYDQAAL"
BASE COUNT         1004 a         1875 c         1914 g         1137 t
ORIGIN      
        1 gcaggaatac gatgcatggc aggcgctctt cgcagcgcag ccgacgctcg tccagcgttt
       61 tctggaagcg caggcacgcg cactggccga tgcgatcccg cagccccacc cggtttcgca
      121 gatgcgcttc acattgcctg atcgcatcct gctcgaagtg ccccaggccg ggccagggca
      181 gtccgttccc attccgtccg accaacgcga gcagttggcc ggcgggctgc tcgatcgcgt
      241 cacgggcggt ggcgcggaca aagtgctgcg ccagcggctg gccgaactgg agcagtcgcc
      301 gaatcgcgcc gtggccgtca gcgccggcct gctgcgacac gtgacggtga tgcacatggt
      361 gcacggcatg ctgcccgccg gccgcagcgt gacgtacacc gcgctgcccg acgaggagat
      421 tccgtcgatc ccggtcggcg gcccactcga acccgaatcc gcgatcactg cggcgacgga
      481 cgcgatcgcc gaagaaggcg agatcgagtc gggccggggc gaactgctcg tgccctacgt
      541 gccggctgcc cgccgcttct atctgccgca gtgggtcgca ttcgacgatc gggatcggct
      601 gctggtgaat tcgatcagcg aggccgaagc catggtcgca tccatgcagc gcttcgtcga
      661 cgtgctgcac gcggccatcg cgctcgcggt gtacatggtg gtcgacgacg agtatcagcg
      721 caagcgctac ggcatgctcg gccagttggt caaccagggc cgcgcactcg cgcgttacca
      781 gaccggcgag atcatccgca cgatcaaggc gcgcgccacg gcgcatgacc tcaatcgcgg
      841 cctcagcctc agcctgccgt actttgacga ccagacgctc tgcgtcgagc cgcacgattt
      901 cgtcgtcatt ccggggggcc gcatcatgtt cgtcgccgcg ctcgtcgtgc gcgccgcccg
      961 gcaggaacag gcaaaggtcg cgcaggatac gcgcttcaat gcctcgacac gccaccatct
     1021 gctgagcctg ctggagatgt tggtgcgggc gttcagttag agaccaggta tcgcatatgt
     1081 tatccgtgat tctgttagta gtcttcttcc tcatcgcata caaactcgcg ccgcgcgaga
     1141 acaaggaaca cgcgcacgtt cacggtgcgg ggcagatcgg cctgctggcc gcgctggcgc
     1201 tgttcggcgt ggactacttc acgtcgtact tctacgcgac gggcgagatg atgcacgccc
     1261 tgcatcccta tggtctgcag cagtatgcct acatcgccgt cgcggtcatc gggtttgcca
     1321 acttcgtgtt cggcgctttg tacatgtatt cactcggcat cttcaacgaa ggcggcggct
     1381 catacaccgc ttcgatgcgt tacctgtggc cgacgctcag cctgatcgtt gccgtcacgc
     1441 tgatccagga ttacgtgctg acgatcgtcg tatcggcgtt gtccggcggc gatcaactgc
     1501 tgtcgatcct cggcgcctac ggccagaact ggctatacca cttcggcatc ggcgcggcgc
     1561 tggccgccgt gacgtggtgg ctcaccattc gcggccgggg cgaatcgtcg cgcgtcgtct
     1621 tcactttgct tggcatcttc atcatgatga caatcacgat ggcgatcggt ctgttcatcg
     1681 cccacgcgaa gagcgtgccg cccgcgccag tcgaagaagc gacgcacagc gtcaccttgg
     1741 ctgaggcgct gctgcacatg ctcactgcat ccatgaaggg tatggtcgcg ctgaccggcc
     1801 tcgaagcgat gtcgaacggc atccagttcg tcatcaacga agatacgggc atcgtcaagt
     1861 ggggcaggaa acatctgccg cgcctgaata aactgtggga cttttatagc ggaaaatcgg
     1921 gcattggccg cttcgtgcag acctcgttcc tgttctacgg cgggctgaca accctgttcc
     1981 tcacgatatt cgccatccgc ttcaacgtgt tcgatggcac agacggacgc acgctggtcg
     2041 gcaacctggc cttcatcggc ttcggccaga ttcaaggcgg cgacctttta ttctgggcct
     2101 accagatact ggccgtgctg ctgctggccg ccgcatcgat gacggcgttt caggatgcgc
     2161 aggccactga gtggcgcgat gtcgctatcg gggaaattcc cgaagtgatc gtctaccgcg
     2221 atccgcgtgg cacgttcacg cgctcggtga cgatcacgtt cggtctggcc gtgttgatca
     2281 tgttcctggt gcgcgggcag acgacgcacg ctgtgccgtt ctacggcgtg ggtgtgttca
     2341 tgccgatcac cgtgatgggc ctcgccatcc gacggcacgt gcagcagcac tatacaggcc
     2401 gtgtccgcaa gtggggcgtc ttcggcgcaa cgatcgccac tgtactggcg ggcatcgtgt
     2461 tcttggggca gatcgtcgga aagtggagcg aaggcggctg ggtcgtgctg atctcgttca
     2521 cgctgctggc gctggccgcg catgccatgc tgctctcacc cttaggctac cgcgagccga
     2581 agcaaattca ccgcatcgtg cgcgacaagg cgcgtgtgca gggcgcgatg gcctccatcg
     2641 tcgagtggca gtcgctcaag atgcaggagt atcgttacgc gctgctcgtt ggcaccgcgc
     2701 gcttcttcga gttgttcggc gtgcgccgcc cggttcgcta cgaagtgcct gtcgtcgccg
     2761 gggattacga ccacgcgctc cacatcgatc atcccgaagc gccgtcgctc ctcgagcaat
     2821 acctggatgc gcgccccgag ccgcacattg gcggcccgcc gaacgtcacg gcggtgggcg
     2881 acctaaaccc tctcgtccaa cccaacggcc ctggtgagag cgtggagtcc gatcgggccg
     2941 ccgaccggca aatagcgtct gcgaacgatg gggagacagc atgatcaagc ggtgagcggc
     3001 gccgcttgag agccactcgg ctatgcgatc tgctccgatc tactccctgc gcgtctcgga
     3061 tgtgttccgg gcgctggaga cctcaccaga ggggcttgcg tcgaatgaag cgcaggcccg
     3121 cctctcgctc tacggcccaa acgtgctgcg cgagccgaag gcagcccctc agtggcggcg
     3181 cttcggcggc cacgtgctgc acccgatggc gctactcctg ttgagcgcgg gcgtcttggc
     3241 atccctcggc ggccggcccg aactcggcat cgtgatctgg ctcatcgtca tggtcaatgc
     3301 cgggttctca ttctggcggg agtatcgggc cgagcaggcg gtgaccgcac tgaagcagtt
     3361 gctcccgtcg tacgcgcgca tcgtgcgaga tggaggcgaa gttcaggtac cttcgagcga
     3421 agtcgtgccc ggcgatgtgc tggtgctggc cgaaggcgac aacatcccgg ccgacgcgcg
     3481 cgttgtcgaa gaatacggcc tgcgcgtgaa caacgcctcg cttaccggcg aagccgtccc
     3541 tgcccgcaag accgccgagg cgtcgctgcg tgaaggactg accgaggttg agcggcccaa
     3601 cttgatcttc gcgagcacat ccgtcgtatc cggcacgggt cgcgcggttg tgtatgcgac
     3661 gggcatggcg acacagttcg ggcgaatcgc caatctcaca caggtcgtcg aagagccgcc
     3721 cagcccgctc cagcagcaac ttcggtggat gacgcgcctc atcaccttcg tggctatcgg
     3781 aatcggcacg gtcgtgtttt tagtcgccgc gctcaatgtg ggcatgccgc gcatcgaggc
     3841 cttgatcctg gccgtgggca tcattgtcgc caccgtgccc gaaggcttgt cccccaccgt
     3901 cgccctgtcg ctggcgatgg ccgtgcagcg gttggcgcgg cgcggcgtgc tggttaagaa
     3961 actcgccacc gtcgaaacgc tcagcgccac atccgtcatc tgcaccgaca aaagcgggac
     4021 gctgacgcag aaccagatga ccgttcgcca ggtgtgggca ggcgggcagc gtttcagcgt
     4081 gtcgggcgca ggctacgagc ctaccggcga gttctcgcca cggcctgatg acactgttgc
     4141 aggcgacttc aaggcgctgc tcggcgcggc gatgttgtgc aacaatgccc gccttatccc
     4201 gccatcgccg gagcatcctc attggacgtg cttgggcgac cagaccgagg cggcgttgcg
     4261 tgtgctcgcg ctcaagggcg gcatggacga tcgcctgttg aacgcgatgc tgccgcgtgt
     4321 ccacgagcta cccttcgatg cgcgccgcaa acggatgacg acgattcatc gcgacagcgt
     4381 gtacggcgag gtagccatgg tcaaaggcgc gccgaaggaa gtgctgcaac tgtgcacgaa
     4441 catcctcatg cacggcgaag tccgtccgct cgacgcggaa acacgggcca cgatcatggc
     4501 cgcgaatgac gacgacgctc gccgcgcgct gcgtgtgata gcgctttccc ggcgcgagtt
     4561 gccgccgcgc tctggcccat attccgtcga agatatcgag cgcgatttga cttttctcgg
     4621 cctcgtagcg atgatggacc cgccgcgccc cgaagtcgcc gatgcggtga aaacctgccg
     4681 ccaggccggt atccgcatcg cgatgatcac cggcgactac ggcctgacgg ctgaatcggt
     4741 cgcgcggcgt gtggggatgc tctccacacc gaatccgcgc atcttgaccg gcacggatgt
     4801 cgaggcgctg aacgatgctg aactgcaggc gctgctgggt gaagaagcag ttttcgcgcg
     4861 gatggcgccc gaccacaagc tgcggctggt cgccgcgttt caggcgcggg gcgacgtggt
     4921 ggccgtgatc ggcgacggtg tgaacgacgc gcctgccctg cgcaaggccg acatcggcat
     4981 cgccatggga cgggcgggca ccgacgtggc gaagcaagct gccgacgtaa tcatcacgga
     5041 cgatgacttc ggtgcgatcg ccggcgccat cgaagagggg cgaacggcat acaacaacat
     5101 tcgcaagttt ctcacttaca tcttcgcgag caacgtgccg gagatcatgc ccttcgtgct
     5161 gatggcgcta ttcaacattc cgctggcact ggccgtcacg cagattctgg cgattgacct
     5221 cggcaccgat ctgctgccgg ccctggcgtt gggcgcggag cggccggagc cggatgtgat
     5281 gcagcgtcca ccgcgctggc gaacgctgcc tctggtggat ggcagcctca tcgcgcgcgc
     5341 actgtggctg ggcgcgattg aaacggtgct gtgctacgtt ggattctttt tcgtcattga
     5401 cggctcgctc aatttggcca tgacgtcgcg ccctgatttg ctgccgttca ccgatcggct
     5461 ggtcgtacgg ggcgggctgg cctacgtgat ggcgacgacc gttttccacg caggcgtcgt
     5521 caccgcgcag atcggcaatg catttgcctg ccggacggag ctgagccacg tgcggcatat
     5581 gggttggttg agcaatcgat tcttgctggc ggggatcgcg gcggagatcg ctattatcct
     5641 atcactggta tacgtcgcgc cactggccgc agtcttcgag cacactcctc tgccaccgga
     5701 atactggctg gtgttactgg cgtacgcgcc gatactttat gtgtgcgagt ggattcgcaa
     5761 gcgcgccgtt cagcgcatcg agcagaggca gttgcgccgg cgccaaggag tcgtcctgat
     5821 atgaaagtca tcatcatggg ctgtggtcgg gtgggcgaag agctgtgccg gattatgctc
     5881 ggcgacgggc atgaggttgc cattatcgac tacgatcagg cagcactgga
//
LOCUS       DBNHXB010000354         4741 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1103070, whole genome shotgun sequence.
ACCESSION   DBNHXB010000354 DBNHXB010000000
VERSION     DBNHXB010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1103070"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="AC162G_14430"
     CDS             <1..1262
                     /locus_tag="AC162G_14430"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077051.1"
                     /translation="RPSGEADTPRLASGRALSVVLTLVMLALGLIVAAAYVGMPLDDP
                     SLVESTPGQLALWLAARNNLDEAATLGTARIALVGMSLLAAGLSGGGLYALARRSASS
                     QRALLWVLLSFDVLVLNVPPVDGSLLYPLPLAGIALALVALFVAPGRVSRTLGVFVVI
                     SALLCFWEVYIALGQATGNTLPLTDFPWKMPHWQNIAEQLLQPARRNGPLLLVGILAE
                     AAFFTWREAFVGFVAGSLLGFVLGTLFAHSKLLERALLPYAIASQTVPILAIAPMIVS
                     ALGAGWLPVAVISAYLTFFPVTINTLRGLLSPDPIALELMRSYAAPAWAVLWKLRVPA
                     ALPYIFTALKVSATGSVVGAIVGELPSSRSDGLAAAILRHSGNYASEPEKLWATIVIA
                     ALVGILFFSAVSLVERWVMRRRVSVES"
     gene            1279..2097
                     /locus_tag="AC162G_14435"
     CDS             1279..2097
                     /locus_tag="AC162G_14435"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077052.1"
                     /translation="MDTSTSGGSQTSNPDQKVVSIKNVHKVFNSGSGDPVVALQDINL
                     EIGANEFVSLTGPSGCGKSTLLRLIADLLQPTSGQLLVNGKPPHQARQDRDYGFVFQA
                     ATLYDWRSVSQNVQLPLEIMGYSPRERAERARAMLKLVELEKFADYHPWQLSGGMQQR
                     VAIARALAFEPALLLMDEPFGALDEFTRERMNMELLNIWGKAGGTIIFVTHSIPEAVF
                     LSSRVVVMSPRPGRITKVTNIDLLRPRNFETREDPRFFAAVTEVRESLREAYGV"
     gene            2130..2963
                     /locus_tag="AC162G_14440"
     CDS             2130..2963
                     /locus_tag="AC162G_14440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077053.1"
                     /translation="MSDSQPDTSDKRYAISDKPVALSRLRYYLPTLVVAVVVLLAWEL
                     IVRVFDIKQFLIPKPTSILEAFADQQSILFDKAFNTLRSAFGGFALGSAGGIVMALVT
                     ARWVMLRQAMMPFAIAANSVPIIAFSPILFNWFGPSNPFSWMAIVAVIVFFPVMINMV
                     RGLVEVDASALEMMRSYAASDFKILFALRIPNALPFLFSALRVATVLSMIGAVVADFF
                     GVERATIGKYITQESASFRFENTWAAILLVSVIGIAFYLVVAVAERSVMPWHVSVRKT
                     E"
     gene            3101..4228
                     /locus_tag="AC162G_14445"
     CDS             3101..4228
                     /locus_tag="AC162G_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5077054.1"
                     /translation="MNRKALGLFGLLIVASLALSACQQAAPAATEAATTEAPQEMAAV
                     RLQLQWVAQSQFAGYYAALAEGYYKEESLDVTFLEGAPEITPQQVCANGGADICIAWT
                     PKALQSREQGADLVNISQVFQRSGTLSVAFAGSGIQSVEDLRGKRVGVWDFGNEHEVF
                     AALRKFGIDPENPDDVTIKIQPFDMTLLLTNEIDAAEAMTYNEYAQVLEQVNPATGEL
                     YQPEDLVVINYNDVGTAMLQDALWATESWLGQEGNEDVAVRFLRASYKGWIFCRDNFD
                     ACVAHVLAAGPTLGESHMRWQLNEINKLIWPSPNGIGQMDKALYDQTVAVAIEGKVLT
                     KEPDAGAYRTDLSAKALEGLEGDTKGAGFTPITVEVRPGGE"
     gene            4306..>4741
                     /locus_tag="AC162G_14450"
     CDS             4306..>4741
                     /locus_tag="AC162G_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR5077055.1"
                     /translation="MARIVRGALFQATWPGSKEKMIEKHVAAAEEAAKHGTQIMCWQE
                     LFYGPYFCQVQDAKHYSWTERIPDGPTTKLAMDLAKRLKMVLIVPMYEEDQTGVYYNT
                     AAVIDADGAFLGKYRKTHIPQVNGFWEKFYFRPGNLGYPVFDT"
BASE COUNT          833 a         1431 c         1603 g          874 t
ORIGIN      
        1 tgcgcccatc gggcgaagca gacacgcctc ggctggcgag cggtcgcgcg ctgtccgtcg
       61 tcctgacgtt ggtcatgctg gcgctcggcc tgatcgtcgc tgcagcttac gtcggcatgc
      121 cgctggacga tccttcgctg gtcgagtcca cgcccggcca gctggcgctg tggctcgccg
      181 cgcgcaacaa cctagatgaa gcggcgacgt tggggacggc tcgcatcgct ctggtcggca
      241 tgagcctgct ggcagccggc ctatcgggcg gcggactgta cgcgctggcc cgacgatcgg
      301 cgtcgagcca gcgcgcgttg ctgtgggtcc tgctgagctt cgatgtgctg gtgctgaacg
      361 ttccgccggt ggacggctcg ctgctgtatc cactgccgct ggccggcatc gctctagcgt
      421 tagttgccct gttcgttgcg cccggccgcg tctcgcgcac gctgggcgtg ttcgtcgtca
      481 tctcggcgct gctgtgcttc tgggaagtgt acatcgcgct cggccaggcg acgggcaaca
      541 cactgccgct gaccgacttt ccgtggaaga tgccgcactg gcagaacatc gccgagcagc
      601 tgctgcagcc ggcccggcgc aatggcccgc tgctgctggt cggcatcctg gccgaagcgg
      661 ccttcttcac ttggcgcgag gcgttcgtcg ggttcgtggc cggcagtctg ctgggcttcg
      721 tcctgggcac gctgttcgcg cattccaagc tgttggagcg cgccctgctg ccgtatgcga
      781 tcgcctcgca gacggtgccg atcctggcga tcgcgccgat gatcgtcagc gcgctcggcg
      841 cgggctggct gccggtggcg gtcatctccg cctacctcac cttcttcccg gtgacgatca
      901 acacgctgcg cgggctgctg tcgcccgacc cgatagcgct ggagctgatg cgatcgtacg
      961 ccgcgccggc ctgggcggtg ctgtggaagc tgcgcgtgcc ggcggcgctg ccgtacatct
     1021 tcaccgcgct gaaagtgtcg gcgacgggca gcgtggtcgg cgcgatcgtc ggcgagctgc
     1081 cgtcgagccg cagcgatggg ctggcggcgg caatcctgcg ccacagcggc aactatgcct
     1141 ccgagccgga aaagctgtgg gcgacgatcg tgatcgcggc gctggtcggg attctgtttt
     1201 tcagcgcagt gtcgctggtc gagcgctggg tgatgaggcg acgagtgagc gtcgagagtt
     1261 agagttcagg agtgaggcgt ggatacgtcg acgagcggcg gttcacaaac cagtaacccg
     1321 gatcagaaag tcgtttcgat taagaacgtc cacaaggtct tcaattcggg cagcggcgat
     1381 ccggtggtgg ccttgcagga catcaacctg gagatcggcg ccaacgagtt cgtgtcgctg
     1441 accggcccgt cgggctgtgg caaatcgacg ctgctgcggc tgatcgccga tctgctccag
     1501 cccacatccg ggcagctgct ggtcaacggc aagccgccgc atcaggcgcg gcaggatcgc
     1561 gattacggct tcgtgttcca agcggcgacg ctgtacgact ggcgcagcgt gagccagaac
     1621 gtgcagttgc cgctggagat catgggctat tcgccgcgcg agcgagccga acgcgcgcgc
     1681 gcgatgctga agctggtcga gctggagaag ttcgccgact atcacccctg gcagctatca
     1741 ggcgggatgc agcagcgcgt cgccatcgcg cgggcgctgg ccttcgagcc ggcgctgctg
     1801 ctgatggacg agccgttcgg cgcgctggac gagttcaccc gcgagcggat gaacatggag
     1861 ctgctcaaca tctggggcaa ggcgggcggc acgatcattt tcgtcacgca cagcatcccc
     1921 gaagccgtgt ttctgtccag ccgggtggtg gtcatgtcgc cgcggccggg gcgcatcacg
     1981 aaagtgacga acatcgactt gctgcggccg cgcaacttcg agacgcgcga agatccgcgc
     2041 ttcttcgcgg cggtgaccga agtgcgcgag agcctgcgcg aggcgtacgg cgtgtgacgc
     2101 atggcgagtg acgggcgaca agtgagacga tgagcgacag tcaacccgat acaagcgata
     2161 agcgctacgc gataagcgat aagccagtcg ctctcagtcg cctgcgctat tacctgccga
     2221 cgctcgtcgt ggcggtggtg gtgctgctgg cctgggaact gatcgtgcgc gtgttcgaca
     2281 tcaagcagtt tctgatcccc aagccgacga gcattctcga agcattcgcc gaccagcagt
     2341 cgatcctgtt cgacaaggcg ttcaatacgc tccgcagcgc gttcggcggc ttcgcgctgg
     2401 gcagcgctgg cggcatcgtc atggcgctgg tcacggcgcg ctgggtgatg ttgcgccagg
     2461 cgatgatgcc gttcgccatc gccgccaact cggtgccgat tatcgccttt tcgccgatcc
     2521 tgttcaactg gttcggcccc agcaacccgt tctcgtggat ggcgatcgtg gccgtgatcg
     2581 tgttcttccc ggtgatgatc aacatggtgc gtgggctggt cgaggtcgat gccagcgcgc
     2641 tggagatgat gcgctcctac gccgccagcg acttcaagat tctgttcgcg ctgcgcattc
     2701 cgaacgcgct gccgttcctg ttcagcgcgc tgcgggtggc gacggtgctg agcatgatcg
     2761 gtgcagtggt ggccgacttc ttcggagtgg agcgggcaac cattggcaaa tacatcacgc
     2821 aggaatcagc cagctttcgg ttcgagaaca cctgggcggc gatcctgctt gtcagcgtga
     2881 tcggcatcgc gttctatctg gtcgtggccg tcgccgagcg cagcgtcatg ccctggcacg
     2941 tgtcggttcg gaagacggaa tgaggctgag gcgcaaaacg caaatcgtgc gacgcgagtc
     3001 aatctctggc tgacgaaagg ggggatgccc ggcgactttc aaccattcat gcggtcttca
     3061 agtgattcga tcttagttca tcttccagga ggagagagaa atgaatcgca aagcattggg
     3121 actgttcgga ctgctcatcg tcgccagtct ggcgttgtcg gcctgccagc aggccgcacc
     3181 cgccgcgacg gaagcggcca cgaccgaggc gccgcaggag atggctgcgg tccgcctgca
     3241 gctgcagtgg gtggcgcagt cgcagttcgc cggctactac gcggcgctgg ccgagggtta
     3301 ctacaaagaa gaaagtctcg acgtgacatt cctcgaaggc gcgccggaaa tcacaccgca
     3361 acaggtgtgc gccaacggtg gcgcggatat ctgcatcgcc tggacgccca aagcgctcca
     3421 gtcgcgcgag cagggcgccg acctggtgaa catcagccag gtcttccagc gctcgggcac
     3481 gctgtcggtg gcgtttgccg gcagcggtat ccagtcggtg gaagacctca gaggcaagcg
     3541 cgtcggagtg tgggacttcg gcaacgagca cgaagtcttc gctgcgctgc gcaagttcgg
     3601 gatcgatcca gagaatccgg acgatgtgac gatcaagatt cagccgttcg atatgacgct
     3661 gctgctgacg aacgagatcg acgccgccga ggcgatgacg tacaacgagt acgctcaggt
     3721 gctggagcag gtcaaccccg ctaccggcga gctataccag cccgaagacc tggttgtgat
     3781 taactacaac gacgtcggca cggccatgct gcaggacgct ttgtgggcaa ccgagagctg
     3841 gctggggcag gagggcaatg aggacgtggc cgtgagattc ctgcgcgcgt cctataaggg
     3901 ctggatcttc tgtcgcgaca atttcgacgc gtgcgtggcg cacgtgctgg cggccggccc
     3961 aacgctgggc gaaagccaca tgcgctggca gctcaatgag atcaacaagc tgatctggcc
     4021 ctcacccaac ggcatcggcc agatggacaa ggcgctgtac gatcaaacgg tcgccgtggc
     4081 gattgagggc aaagtgctga ccaaagagcc ggacgcaggc gcgtatcgca ccgacctatc
     4141 cgcgaaggcg ctggaggggt tggagggcga cacgaagggc gccggcttca cacccatcac
     4201 ggtcgaagta aggccgggcg gcgagtaatt ccctcagttc cctgagttcc ccacttcttg
     4261 taacaggaac tgaaggaact cagggaactc ttttcggagg tgagcatggc acgtatcgtt
     4321 cgcggcgcgc tgttccaggc gacgtggccg ggcagcaaag agaaaatgat cgagaagcac
     4381 gtcgccgccg ccgaggaagc ggcgaagcac ggcacacaga tcatgtgctg gcaggagttg
     4441 ttctacggcc cgtacttctg ccaggtgcag gatgcgaagc actacagttg gaccgagcgc
     4501 atccccgacg gcccgacgac caagctggcg atggacctgg cgaagcggct caagatggtg
     4561 ctgatcgtgc cgatgtacga ggaagaccag acgggcgtat actacaacac cgccgccgtg
     4621 atcgacgccg acggcgcgtt tctcggcaag tatcgcaaga cgcacatccc ccaggtcaac
     4681 ggcttctggg agaagttcta cttccggcct ggcaatctcg gttatccggt cttcgacact
     4741 g
//
LOCUS       DBNHXB010000355         7050 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4291810, whole genome shotgun sequence.
ACCESSION   DBNHXB010000355 DBNHXB010000000
VERSION     DBNHXB010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7050)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4291810"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="AC162G_14455"
     CDS             <1..856
                     /locus_tag="AC162G_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5077056.1"
                     /translation="LAAVAALLIILFNAETTRLIPLYAIGVFLSFTLSQSGMVVRWQK
                     VGKMKPGEEIEVHGSVMRYDPHWRGKRIVNAIGALMTGVVMIVFAASKFADGAWIVVL
                     LVPALVFVFFRIHHHYKHVAAVLGQGSRWPNIRPRPVKTLVLVDDVHSGTINTINFAK
                     SLGVPWTAVHVAIDPEKAERTRRKWRERIGDEAYLTVLPSPYRNLVDPVHEYIEQLLK
                     ELPKGYIHVIVGHLVMDNLLEQALHQNAAVSLSLALQDLQRVAVTTVPHQLGQRENGV
                     APVRHPTP"
     gene            complement(846..986)
                     /locus_tag="AC162G_14460"
     CDS             complement(846..986)
                     /locus_tag="AC162G_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077057.1"
                     /translation="MIDKLFFDQVREDAVADAELQQAARANTIENFAYVFDTALEGFF
                     RE"
     gene            1017..1223
                     /locus_tag="AC162G_14465"
     CDS             1017..1223
                     /locus_tag="AC162G_14465"
                     /inference="COORDINATES: protein motif:HMM:NF014059.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin AF2212-like protein"
                     /protein_id="HIR5077058.1"
                     /translation="MAVLTTRAVYKDGVLKPTTKLDLPDGATAEVQISALPLAVAVDP
                     KDALLDDEAALQAMCSEFAEEEMF"
     gene            1253..2848
                     /locus_tag="AC162G_14470"
     CDS             1253..2848
                     /locus_tag="AC162G_14470"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR5077059.1"
                     /translation="MSPPRKVIVSVVCALAALALFTLATRGASPSDPVPYEPLPPDVK
                     VETVVPGANQLVAMAFTPDGRLLYTERTGKVRVVVNGVLQPDAVMTFPVKTEGERGLL
                     GIAVDPNFAANHYVWVYLTKQTAGPQCSSIENRVVRFTLGDDHRGYDETVAGCFPVNP
                     FATIHNGGNLHFGPDGMLYVTVGNNDDVDDHIDPAQSLGSPLGKLHRFIPAVPLGIPG
                     DNPFYNTPGAVQSIYARGLRNSFDFTFDPIGGAIFATENGDDCDDEVNRILPGGNYGW
                     RPNYPCDDESPTGPDPAYNTLPPLIYWTPSLAPTGIAVYTGTIIPEWQGDLFMCGFKD
                     ATTALYHFKLNAARTAIVSHAILTDTITHQRIRCRTDILVGPDGALYFSEGGGWVNGP
                     IKRLIRKSSFSATTVSPQRLNPASGTLLEYTIDLRHIGSLTNTFALIVDVPSAIAYLS
                     DAQAAQGTLVKEPYRMSWSGTIPAGERWTAAYQVYLAGPPEPHVLTNTVRLTAPGATT
                     IVLTPTVIVNGQIAFLPVIQRSP"
     gene            complement(3030..3515)
                     /gene="paaD"
                     /locus_tag="AC162G_14475"
     CDS             complement(3030..3515)
                     /gene="paaD"
                     /locus_tag="AC162G_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HIR5077060.1"
                     /translation="MTALTVEEIWRSLEEVKDPEIPVVSLVEMGIVRGVEVSGGKVVV
                     SMTPTFAGCPALHVMKAEVEQRLREMGAESVETKWVYSPPWTSDWIADEARAKLKAFG
                     LAPPVKHRGDVNLILLEEAACPYCGSRNTTIKNTFGPTLCRAIYYCNDCQQPFEQFKP
                     L"
     gene            complement(3619..4377)
                     /gene="paaC"
                     /locus_tag="AC162G_14480"
     CDS             complement(3619..4377)
                     /gene="paaC"
                     /locus_tag="AC162G_14480"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HIR5077061.1"
                     /translation="MTPGVERAIAAQLLAMADDELILGHRDSEWTGHGPILEEDIALS
                     NIAQDELGHAILWHNLRKEITGDEPDVVVFWRDAQAYRNVQMVELPKGDYAFTIMRQY
                     LFDAAEMARLAQLVESRYQPMAEVAAKIRTEEIYHTRHTSTWIKRLGLGTEESHRRTQ
                     TALDELWAYALQLFVPLPEEASLVAKGIVSEAGEVQAAWEDMVAPFLKHAGLIVPRTR
                     HPIAMSREQHTGHLTALLAEMQEVARLEGPEVRW"
     gene            complement(4374..4961)
                     /locus_tag="AC162G_14485"
     CDS             complement(4374..4961)
                     /locus_tag="AC162G_14485"
                     /inference="COORDINATES: protein motif:HMM:NF018002.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein"
                     /protein_id="HIR5077062.1"
                     /translation="MSQYVEFGMDTQWPRYEVFEKERPDWPHRNAGSVHAPDGEMALL
                     NARDVFVRRPSCLSLWVVPSDQVFSKTAQELAEDDSWRNAPADANAPLEKYLAFQKQG
                     QRAGETFVIHVGEVEARSPAEAMSRALETFSHENVFVWWVCPDRAVTKSDTDDVASMF
                     EPAHSKVYRQPNFYRVVMQMQQFKAGLAFIGEEEE"
     gene            complement(5013..5945)
                     /gene="paaA"
                     /locus_tag="AC162G_14490"
     CDS             complement(5013..5945)
                     /gene="paaA"
                     /locus_tag="AC162G_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HIR5077063.1"
                     /translation="MTDEEHLQEFEARIARGEKIEPGDWMPGEYRKQLIRMISQHAHS
                     EIVGMLPEGKYVTRAPNLRQKLVLLAKVQDEGGHGQYLYHAAETLGATREEMVDALLA
                     GKAKYSSVFNYPSLSWADMGAIGWLVDGAAIMNQTMLAKCSYGPYSRAMVRICTEEGF
                     HKKQGMEMVIRYATQGTPKQKAMMQDAINRWWWPSLMMFGPHDANSPNSPQLIRWGIK
                     TKTNDQMRQEYVNELVPALHVLGLSVPDPDLRYDEKTGNWLFGEIDWDEFWRVVKGDG
                     PCNKERLEARRKAHAEGQWVREALTAYAQKKAQG"
     gene            complement(6058..7050)
                     /gene="hpaD"
                     /locus_tag="AC162G_14495"
     CDS             complement(6058..7050)
                     /gene="hpaD"
                     /locus_tag="AC162G_14495"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HIR5077064.1"
                     /translation="MPSFNITRVGHAEIRVTDLGRARAFYVDALGLLEIDKDDEHLYL
                     GGMEERDKYCLLLRKAESSGIGHLAFRVAGPDDLDKIARVYEENGLPTRWIEPNTLER
                     GQGRALRVQDPSGLPFEFYHEMEQRERLLQRFELYRGAHVMRIDHFNCQAPNVQAAYD
                     WWTKVMGFWCSEYTVSDDEPERLWAVWLHRKQNVHDLALMNGIGPRLHHVGMWLQDTN
                     SVLRACDILASLGMVGSMERGPGRHGLSNAFFLYLRDPDLNRIELYTNDYIIPDPDFE
                     PIKWKLSDPRRATFWGHWAPRSWFDEASLVEDIRTGQLLPTQEPLMAGRPEHVT"
BASE COUNT         1247 a         2312 c         2047 g         1444 t
ORIGIN      
        1 gctcgcggcg gtggccgcgc tgctgatcat cctcttcaat gccgagacga cgcgattgat
       61 cccgctgtac gccatcggcg tgttcctctc gttcacgctg tcccagtccg gcatggttgt
      121 gcgctggcag aaggtcggca agatgaagcc gggcgaggag atcgaagtcc acggctccgt
      181 catgcgctac gacccgcact ggcggggcaa gcgaatcgtg aacgccatcg gcgcgctgat
      241 gacgggtgtc gtcatgatcg tctttgccgc ctccaagttc gccgatggcg cctggatcgt
      301 cgtcctgctc gtgcccgcgc tcgtattcgt cttcttccgc atccaccacc actacaagca
      361 cgtggccgcg gtgttgggac agggatcgcg ctggcccaac atccgtccca ggccggttaa
      421 gacactcgtg ctcgtcgacg acgttcacag cggcaccatc aacacgatca actttgccaa
      481 gtcgctgggc gtgccgtgga ccgccgtgca cgtcgcgatc gatcccgaga aggccgagcg
      541 gaccaggcgc aagtggcgtg agcgtatcgg cgacgaggct tatctgaccg tcctgccgtc
      601 accttaccgc aacctggtgg atccggttca cgagtacatt gagcaactgc tgaaggaatt
      661 accgaagggt tacattcatg tgattgtcgg ccacctggtg atggacaacc tgttggagca
      721 ggcgctgcac cagaacgcgg ccgtcagcct gagcctggct ctgcaggact tgcagcgagt
      781 ggcggtgacg accgtgcccc accaactcgg ccagcgcgag aacggtgtcg cgccggtaag
      841 gcatcctact ccctgaagaa tccctccagc gctgtgtcga agacgtaagc gaagttctcg
      901 atggtgttgg cgcgcgcggc ctgctgcaac tccgcgtcgg cgacggcgtc ttctcgcact
      961 tgatcgaaaa acagtttatc tatcatttgg tgtataatct ccgttcgatg tgcccaatgg
     1021 ctgttctcac cacacgcgcc gtctataagg acggcgttct gaaaccaacc acaaaactcg
     1081 atctgcccga cggtgctact gccgaggttc agattagcgc actgccactc gccgtcgcag
     1141 tcgatcccaa ggatgctcta ctggatgacg aggctgcgtt gcaggcaatg tgctccgaat
     1201 tcgcggaaga ggaaatgttc taaatctcgt cagttaaagg aggatcagcc tgttgtctcc
     1261 cccacgcaaa gtgattgtgt ccgtcgtatg cgccctggcc gcgcttgctc ttttcaccct
     1321 agccacgcgc ggcgcttccc ccagtgatcc agttccctac gaaccgcttc cgcccgatgt
     1381 caaagttgaa accgttgtgc ccggcgcgaa ccaactggtg gcgatggcct tcacgcccga
     1441 tgggcgtttg ctgtacaccg agcgcacggg caaggtgcgt gtggtggtga atggtgtctt
     1501 gcagcccgac gccgtgatga cgtttcccgt gaagaccgag ggtgaacgcg gcttgctggg
     1561 tatcgcggtc gatccaaatt tcgccgccaa tcactacgta tgggtgtacc tcaccaaaca
     1621 aacggccggc ccgcagtgca gttcgatcga aaaccgcgtg gtgcgcttta cgctgggtga
     1681 tgatcatcgg ggctatgacg aaaccgtagc cggttgtttt ccggttaatc cattcgcaac
     1741 tattcacaac ggcggcaatt tgcactttgg gccagatggg atgttgtatg tcacggttgg
     1801 caacaacgac gacgttgacg atcatatcga tccggcgcaa agtctaggtt cgccgttagg
     1861 caagctgcat cgcttcatcc cggctgttcc gctgggcatt ccaggggaca atccttttta
     1921 caacacgccg ggcgctgtgc aaagcatcta cgcccgcggc ttacgcaact cgttcgattt
     1981 cacgttcgat cccatcgggg gcgcgatctt cgccacggag aacggcgatg actgcgacga
     2041 cgaagtcaat cgcatcttgc ccggcggcaa ctatggctgg cgacccaatt acccgtgtga
     2101 tgacgaaagc ccgaccggtc ctgacccggc ctataacacc cttccaccct tgatctactg
     2161 gacgccctcg ctggccccga ccgggattgc ggtctatacc ggcacaatta ttccggaatg
     2221 gcagggcgat ttgttcatgt gcggcttcaa agatgccact acggcgcttt atcatttcaa
     2281 attgaacgca gcgcgcacgg ccatcgtttc gcacgcgatc ctgaccgaca cgatcaccca
     2341 tcagcgcatc cggtgccgca ccgatatatt agtcggtccc gatggggcgc tgtatttttc
     2401 cgaaggcggc ggctgggtca atggcccgat caagcgcttg atacgcaagt cgtcgttcag
     2461 cgcgacgacg gtcagcccac agcggctcaa tccggcgtca gggacgctgc ttgagtacac
     2521 catcgatctg cggcacattg gatcgctcac gaataccttt gcgctcatag ttgatgtgcc
     2581 gagcgcaata gcgtatctca gcgacgcgca ggccgcacag gggactctgg tgaaggaacc
     2641 ctatcgcatg agctggtcgg gcactatccc ggccggtgag aggtggacgg cggcgtatca
     2701 ggtgtacctc gccggcccgc ccgagccgca tgttcttacc aacacggtgc gcctcaccgc
     2761 gcccggcgca acgaccatcg tgttgacgcc gaccgtgatc gtcaatggac agatcgcctt
     2821 tctgcccgtc atacagcgca gtccatagcg gtggtattgg ggcgatccaa atcgccatgc
     2881 tagcagcttg tcgaagaggg agcgggccgg gctcccacgc ccggcagttg gccttgaaat
     2941 gcgctaaatc ggcggcgtgc ccgcttcgca ctgctgcgct gcggggtgct tcgcaaggag
     3001 gcgcctctgc tgggttctcc gacagccggc tagagcggct tgaactgctc gaacggctgc
     3061 tggcagtcgt tgcaatagta gatggcgcgg cacagcgtgg ggccgaatgt gttcttgatc
     3121 gtcgtgttcc gcgagccgca gtacgggcag gcggcttctt cgagcagaat caggttgacg
     3181 tcgccgcgat gcttcaccgg cggcgcgagg ccgaacgcct tgagcttggc gcgcgcttcg
     3241 tcggcgatcc agtcgctcgt ccacggcggc gaatagaccc acttcgtctc gaccgactcg
     3301 gctcccatct cgcgcagacg ctgctcgacc tcggccttca tcacatgcag cgccgggcag
     3361 cccgcgaatg tcggcgtcat cgatacgacg accttgccgc ctgacacctc tacgccccga
     3421 acaatgccca tctcgacgag cgacaccacc ggaatctccg ggtccttcac ctcttcaagc
     3481 gaccgccaga tttcctcaac ggttagtgct gtcatagcag tatttcacca cggagacaca
     3541 cagagcacga gttctccaat ccccaatccc caatatccaa tctctaatct ccaatctcca
     3601 atccccgacc cccaactact accacctgac ctccggtcct tccagtcgag ccacttcttg
     3661 catttccgcc agcaacgccg tgagatgccc cgtgtgctgc tcgcgcgaca tggcgatcgg
     3721 atgccgtgtc ctgggaacga tcaacccggc atgcttgaga aagggcgcga ccatgtcttc
     3781 ccaggccgcc tgcacctccc cagcttcaga aacgatacct ttggcgacga gcgatgcctc
     3841 ctcgggcagc gggacgaaga gctgcagcgc gtacgcccac aattcgtcga gcgcggtctg
     3901 cgtgcgccgg tggctctcct ccgtgcccag acccagccgc ttgatccagg tgctcgtgtg
     3961 ccgagtgtgg taaatctcct cggtgcgaat cttggccgcc acttcggcca tcggctggta
     4021 gcggctttcc accagttgag ccaggcgagc catttcggcc gcgtcgaaga gatactgccg
     4081 catgatggtg aaggcgtaat cgcccttcgg cagctctacc atctggacgt tgcggtacgc
     4141 ctgcgcgtcg cgccagaaca cgacgacgtc cggctcgtcg cccgtgatct ctttgcgaag
     4201 gttgtgccac aggatggcgt gccccagctc gtcctgcgcg atgttagaca gggcaatgtc
     4261 ttcttccaag atcggcccat ggcccgtcca ctccgagtcg cggtgaccga ggatcaactc
     4321 gtcgtcggcc attgccagca gctgcgccgc gatggcgcgc tcgacgccgg gagtcattcc
     4381 tcttcctcgc cgatgaacgc caggccggcc ttgaactgct gcatctgcat cacgacgcgg
     4441 tagaaattgg gctggcggta caccttcgag tgcgccggct cgaacatgct cgccacgtcg
     4501 tctgtgtcgc tcttcgtcac cgcccggtcg gggcacaccc accagacgaa gacgttctcg
     4561 tggctgaagg tctccagcgc gcggctcatc gcctcggcgg gcgagcgcgc ctcgacctcg
     4621 ccgacgtgga tcacaaaggt ctcgcccgcg cgctggcctt gtttctggaa ggcgagatac
     4681 ttctccagtg gcgcgttggc gtccgccggc gcgttccgcc aggaatcgtc ttccgccagc
     4741 tcctgagccg tcttggaaaa gacctgatcg ctcggcacga cccacaggct caggcagctc
     4801 ggccgccgca cgaagacgtc gcgcgcgttg agcaatgcca tctcgccgtc aggcgcgtgg
     4861 accgagccgg cgttccgatg cggccagtcc ggcctctcct tctcgaaaac ctcgtaacga
     4921 ggccactgcg tgtccatgcc gaactctacg tactgactca ccgatccatc gcttcttctg
     4981 aagtttggag tttggagttt gacttttgaa ctttaacctt gcgccttctt ctgcgcatag
     5041 gccgtcagcg cctcgcgcac ccattgcccc tcggcgtgtg ccttgcgccg cgcttcgagg
     5101 cgctccttgt tgcacggccc gtcgcctttg acgacgcgcc agaactcgtc ccagtcgatt
     5161 tcgccgaaga gccagttgcc ggtcttctcg tcgtaacgca ggtcaggatc gggcacgctg
     5221 agtccgagca cgtgcagcgc gggaaccaac tcgttgacgt attcctgccg catttgatcg
     5281 ttcgtcttcg tcttgatgcc ccagcggatc aactgcgggc tgttaggaga gttcgcgtcg
     5341 tgcggaccga acatcatgag cgacggccac caccagcggt tgatcgcgtc ctgcatcatc
     5401 gccttctgct tcggcgtgcc ctgcgtcgcg tagcggatga ccatctccat gccctgcttc
     5461 ttgtggaagc cttcctcggt gcagatgcgc accatggcgc gcgagtacgg gccgtacgag
     5521 cacttggcga gcatcgtctg gttcatgatc gccgcgccgt cgaccagcca tccgatcgcg
     5581 cccatgtcgg cccagctcag gctcggataa ttgaacaccg acgaatactt ggccttgccc
     5641 gccagcagcg cgtcgaccat ctcctcgcgc gtcgcgccga gcgtctcggc ggcgtgatac
     5701 agatactggc cgtggccgcc ttcgtcttgc accttggcca gcagcaccag cttctgccgc
     5761 aggttgggcg cgcgagtgac gtatttgccc tcgggcagca tgcccacgat ctcgctgtgc
     5821 gcgtgctgcg agatcatgcg gatgagctgc ttgcgatact cgccgggcat ccaatcgccc
     5881 ggctcgatct tctccccgcg cgcaatgcgc gcttcgaact cttgcaagtg ttcttcgtca
     5941 gtcatacaac ctcctggcgt atcgcctatc gcctatcgca tttcgcatat cgcatatcgc
     6001 ccatcattaa tcgcttcgag cgataagcga tacgccatct gccatccgcc atctgtatca
     6061 cgtgacatgt tccggccggc cggccatgag cggctcctgc gtcggcagca actgcccggt
     6121 acggatgtct tcgacgagtg atgcctcatc gaaccaactg cgcggcgccc agtgacccca
     6181 gaacgtcgcc cggcgcggat cgcttagctt ccacttgatc ggctcgaagt ccggatccgg
     6241 aatgatgtag tcgttcgtgt atagctcgat ccggttcaaa tcgggatcgc gcaggtacag
     6301 gaagaaggcg ttgctcaatc catgccggcc cgggccgcgc tccatcgacc cgaccatgcc
     6361 cagcgaagcc aggatatcgc acgcgcgcag cacgctgttc gtgtcttgca gccacatgcc
     6421 gacgtgatgc aggcgcgggc cgatgccgtt catcagcgcc aggtcgtgta cattctgctt
     6481 gcgatgcagc cacacggccc acagccgctc cggctcgtcg tcgctgacgg tgtactccga
     6541 gcaccaaaag cccatgactt tcgtccacca gtcgtacgcc gcctgcacgt tcggcgcctg
     6601 gcagttgaag tggtcgatgc gcatgacgtg cgcgccgcga tacagctcga agcgctgcag
     6661 cagccgctcg cgctgctcca tctcgtggta gaactcgaac ggcaggccgc tcggatcctg
     6721 cacgcgcagc gccctgccct gcccgcgctc gagcgtgttc ggctcgatcc agcgtgtggg
     6781 taggccgttc tcttcgtata ccctcgcgat cttgtcgaga tcgtccggcc cggcgacgcg
     6841 gaaggcgaga tgcccgatgc cgctgctctc tgccttgcgc agcaagaggc aatacttgtc
     6901 gcgctcctcc atcccgccca ggtaaaggtg ctcgtcgtcc ttgtcgatct cgagcaagcc
     6961 cagcgcatcc acgtagaacg ccctggcgcg ccccagatcg gtgacgcgga tttcggcgtg
     7021 gccgacgcgg gtgatgttga atgacggcat
//
LOCUS       DBNHXB010000356        16939 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3285115, whole genome shotgun sequence.
ACCESSION   DBNHXB010000356 DBNHXB010000000
VERSION     DBNHXB010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 16939)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16939)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16939
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3285115"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(307..495)
                     /locus_tag="AC162G_14500"
     CDS             complement(307..495)
                     /locus_tag="AC162G_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HIR5077065.1"
                     /translation="MAIDPVCGMEVDEKTAKFKAEYKGETYYFCSPGCRYTFNERPED
                     FVGPNRKGYVPMEGHGHH"
     gene            complement(569..3055)
                     /locus_tag="AC162G_14505"
     CDS             complement(569..3055)
                     /locus_tag="AC162G_14505"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005832210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR5077066.1"
                     /translation="MTTKQVTLPITGMTCANCVATIERATKKMPGVVAANVNLSSERG
                     VFEFDPGALSEQEVIARIEDIGYGVARAEADIPIQGLHDDRDAAVVQEALSQVDGVLQ
                     ASVSFATEKARVEYLPTMVGQGDLRRAIEAAGFQAIVAEGASGEDAERKAREAEFAHQ
                     RRRLAVGLIFTIPLFALSMVNDAWHAGFLEIPQFLLPLFHWSGLGWLFLALATPVQFY
                     VGRDFYVGAWKALKNRAANMDVLVAMGSSVAYFYSLAVLLGLVEGHMYFETAAVIITL
                     IVLGKYLEAKAKGRTSEAIKKLMGLRAKTARIVRDGAEMEVSIDDVRVGDTVVVRPGE
                     KIPVDGVVIEGRSSVDESMITGESMPVEKGSGDRIVGATVNKQGLLKFEATQVGKNTV
                     LAQIVRMVEQAQGSKAPIQRLADQISAVFVPAVIAIAAVTFAGHMLLNPGAGFEFAMK
                     AAVAVLVIACPCAMGLATPTAVMVGTGKGAENGVLFKSSEALERLGNAQVIALDKTGT
                     ITEGKPKVTDVVISDQLSVVSDQSAVSTDHGSLTTDQLLRYAASVERGSEHPLAAAIV
                     EAASTRSVTLSEPQAFRAISGRGVSATVDGRRVLLGNLALMQEQQVHLNGLAPEVERL
                     QAEAKTAMLLAIDGEAAGVIAVADTVKPGSKEAIASLHAQGIEVVMITGDNRRTAEAI
                     AREVGIDRALAEVLPGDKAATVKQLQEGNLRKVVAMVGDGINDAPALAQADVGIAIGT
                     GTDIAIEAAGVTLMSGDLRGVPKAITLSRGTLRTIKQNLFWAFFYNVILIPVAALGAL
                     IPMLAAAAMAFSSVFVVSNSLRLRGMKVKA"
     gene            complement(3182..3460)
                     /locus_tag="AC162G_14510"
     CDS             complement(3182..3460)
                     /locus_tag="AC162G_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HIR5077067.1"
                     /translation="MDAQSKDTLKRRLKSISGHVAGIERMVDQDAYCIDVIKQVQAVQ
                     AALNKVNDLILKGHLNSCVIDAVRGDDKKRREQVLDEIVDVFEMAQKI"
     gene            complement(3582..4511)
                     /locus_tag="AC162G_14515"
     CDS             complement(3582..4511)
                     /locus_tag="AC162G_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HIR5077068.1"
                     /translation="MRTLIQPSTFSIVAYDPDAQEWGVATQSKFLAVGSAVPWAQAGA
                     GAVATQSYANTSFGPKGLALMASGLSAQETLDRLLTEDEGREHRQVGLVDAQGNVATF
                     TGRECLEWAGGMTGRHYAVQGNILVSKGTVSAMVQAFEQARGELADRLVAALAAGQKA
                     GGDRRGQQSAALLVVREKGGYAGFNDRYIDLRVDDHSRPIARLRALLDLHHLYFKRPD
                     PESLIPIDKKVARELQRIARQSGHYTGRLSGDYDDATRKAVEKLVGVENLEERWQPDE
                     RIDPVVLEFLQKAYPAQKRPATGSGKRAKKSRR"
     gene            complement(4587..4976)
                     /locus_tag="AC162G_14520"
     CDS             complement(4587..4976)
                     /locus_tag="AC162G_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077069.1"
                     /translation="MSTPFDIFKEMLLQAAGGALARRGYTLQDDAIQIRSGLYRFARA
                     LEDGALALVDVQMLFYSGGGPSRFEVKVWRSDRPSDKTRLGVWLHGQAVETLADDLGW
                     WEFVSGPELEESLRDAVSGLEQMWKEE"
     gene            complement(5005..5154)
                     /locus_tag="AC162G_14525"
     CDS             complement(5005..5154)
                     /locus_tag="AC162G_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077070.1"
                     /translation="MYIIRHLSPFIVGLYAGEGGTQKAIGVSDVMYCGKCHPREQPVP
                     LPELK"
     gene            5389..5961
                     /locus_tag="AC162G_14530"
     CDS             5389..5961
                     /locus_tag="AC162G_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077071.1"
                     /translation="MSIETGSQALSQFERRLLAGNAKARIKGLFRPHIVIHDAASEVL
                     GEIRAGLVFDRFPHRARCTALGIEMSLAVAGFLNQSSHLLVNGDPMLRVSHPALSRRY
                     VAEDWQGDTYQLHRRGRPWHRRFNLMRSAGEEEMICFECASLCRETVLSIVAQPELKP
                     GVIAGLALLLYYNWYLSLRTAFISAGGAGG"
     gene            6118..7371
                     /locus_tag="AC162G_14535"
     CDS             6118..7371
                     /locus_tag="AC162G_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015174421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5077072.1"
                     /translation="MSEGAAELARERVDAIYRSDSRRIFATLIRLLGDFDLAEEALQE
                     AFTAAVAQWPQAGVPANPRAWLVSVGRFKVIDTLRRSARFHASLAALAEQLDADTNDT
                     EGWEGEGVEDDRLRLIFTCCHPALSQDAQVALTLREVCGLATEEIARAFLVAPSTLAQ
                     RIVRAKAKIRDARIPYEVPSRAELPDRLDTVLQVIYLVFNEGYSASWGESLTRPDLSS
                     EAMRLGRLLVELLPEPEALGLLALMLLHESRRASRVSPEGDLVLLEDQDRSLWNREQI
                     AEGTALVERTLSSGRLGPYTLQAAIAVVHAAAPDFAATDWAQIVSLYDMLAQVAPSPV
                     VEINRAVAVAMHDGPLAGLALIDAILARGDLANYHLAHAARADLCRRLGRTAEARASY
                     QRALALTQQEPERRFLERRLSKLSD"
     gene            7518..7868
                     /locus_tag="AC162G_14540"
     CDS             7518..7868
                     /locus_tag="AC162G_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020552803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR5077073.1"
                     /translation="MQFLVYDPHYNPEAATPPTPELLAEMGEFIGEAMQAGVLVTTGA
                     LQPKGTRLRLSGGKFTVTDGPFIELKELMGGWAVIRTKSLEEAIEWCKRFRKIVGDGE
                     TEIVQVFGPEDSPA"
     gene            8399..9235
                     /locus_tag="AC162G_14545"
     CDS             8399..9235
                     /locus_tag="AC162G_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5077074.1"
                     /translation="MSTTTTNKPALTAGNYASVNGLNMYYEIHGAGEPLILLHGGVGA
                     IEMFGEVLPLLAEGRQVIAVDLQAHGRTADIDRPLSFALMADDIAALIKHLGLQKADV
                     MGYSLGGGVALQVAIRHPNAVRKLVVVSTPFKRDGWYPEVLAGMEQMGPEAAEPMKQT
                     PMYQLYARVAPKPEDWPVLLTKLGELLRQGYDWSEDVAAIKAPTLIVVGDADSVRTSH
                     AVELFELLGGGKADAGWDGAGMSNARLAILPSTTHYNIFSSPALASTVTPFLNAPMSQ
                     AQ"
     gene            9536..12178
                     /locus_tag="AC162G_14550"
     CDS             9536..12178
                     /locus_tag="AC162G_14550"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5077075.1"
                     /translation="MTTPLLTTKLHIPPLLPDRVPRLRLIRRLDEGLRSGNKLTLVSA
                     PAGYGKTTLVAEWLRHLQDADDAALAVSWLSLDEGDRDPARFWAYLVAALQAVDPAIG
                     QTAQVMLHSPQPPPPESFLTALINEIAAIRRSFVLVLDDYHLVEALPIHQQLTFLIEH
                     QPTQMHLVIATREDPPLPLSRLRARGQTVEIRQDDLRFSREECADFLKSVMALDLSQD
                     DIAALERRTEGWIAGLQMAALSLQHTTDSRGFVQSFTGSHRYILDYLIEEVLSKQPSH
                     LQDFLLKTAILDRLSASLCDTVAERSDSHEVLQHLEAANVFIVPLDPGRDWYRYHHLF
                     TELLRHRLRLAPGITEETLHQRASYWFEQNQYPFEAVQHALAAADWERSAQLIHTESD
                     GMLKRGEVATLLGWLKKLPEEQVAARPGLSLTFSWPLILSGQLETAERLLTHAEQGAG
                     KDPHVLGQIAAAQAYIARSRGDDQRTVAMSQRALSLLPETDVALRELLAVNLGIAHWH
                     NGQLEEADNVLNEAWRLSVGLGNRYAEMTIQVFLNRILAARGLLQQAYQAYQPLVELE
                     DQVPILALAHLDLSALRYEWNDLEACATHIEKSIAVSERTRNGEFQISGHIQMARLRL
                     AYGDANGAATALRHAEQLLQAHSVTPLTRARNAAAHVQVALFQGDLSGASAWARDAGD
                     HADVHPFYPFLGLSRARLLLAQNRQAEALDCLKDRFEIAARAGWSYGQIAVRVLQAVA
                     AGQSEEALDFLIQALTWAQPGGFIRTFVDAGGALAPLLEEAALRGVMPEYVGTLLAAL
                     GSQAKVLQLTKATGVSPLVEPLSEREIEVLRLVAAGLSNREIAKKLVISPGTAKTHIH
                     NLCGKLGVRNRTEAATRAKELGLV"
     gene            12324..12905
                     /locus_tag="AC162G_14555"
     CDS             12324..12905
                     /locus_tag="AC162G_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077076.1"
                     /translation="MNKSRLIWGIICLILAAALGVLDIVLPPDRLMFQVGDQNMYWIP
                     AIVLGIVGIVLLATAGSSGQATAEAKQPAVVQNPEKAALNKRLENIGWGLFLIMLGGF
                     ALVPDESVPKGLWSIGVGVIMLGLNVTRYFYKIKMSGFTTILGILALLGGIAELLGFT
                     SLEGGLLLIILGAYLLLKPWFEKRQLFGKAEES"
     gene            12924..13145
                     /locus_tag="AC162G_14560"
     CDS             12924..13145
                     /locus_tag="AC162G_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077077.1"
                     /translation="MLKVEIQVKGCIDKDWSDWFAGLAIAHPEENYTVLTGSLPDQAA
                     LFGLLTQIRNLGLPLVSVSSEEQNQTEDV"
     gene            13168..14145
                     /locus_tag="AC162G_14565"
     CDS             13168..14145
                     /locus_tag="AC162G_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017154260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077078.1"
                     /translation="MLEVDNLVKKYGDFEAVKGISFSVEEGEVFGLLGPNGAGKTQTI
                     SMLTGVIQPTSGTARIGGYDIRTRMGEVKKINGLVPQDLALYPTLSARANLNFFGRIY
                     GLRGKQLKERVDDVLRVVALAERADQAVDKYSGGMKRRVNIGAGLLHHPRLFFLDEPT
                     VGVDPQSRNQIFESVQQLNRERGMSIVYTSHYMEEVELLCNRVAIIDHGQIIAMDTIK
                     NLIAMLGGGVIHLGLSRVDDALLKDLSALPGVKRTSLAPLPVAPANGEAAPQAKGPIV
                     KIECENSQRALVSVVSYLGGHGLGVTSLETLEPNLESVFLHLTGKSLRE"
     gene            14151..15425
                     /locus_tag="AC162G_14570"
     CDS             14151..15425
                     /locus_tag="AC162G_14570"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077079.1"
                     /translation="MNLLSVTLKDLQVFVRDRGAVFLLFLLPFAFIAVLSLATQGVRL
                     GESGDGGQTRVPLTVVNNDAQGQAAQEFLAALNDTGKVQIDLGGPQAKVERQLNDATL
                     RYALFIPVDFSAKLAAGDQVSLRLALHPYANQADLLTVERAIVRAAREYLIMGYLNDG
                     LEQMAAMQAANPQAASTFSKERIQQQIEAQQAQAEQRPLITVTTTTPAVKGEGQAAAE
                     ENLPTYGQFLVVGMAVLFVFLAAQNTARSIYEEKREGTFRRLMAAPISKASLLGGKLL
                     PNFLLVLVQVAVILVTGGFVIQLFGVPPLNLGSDLLGLVVICAATALCSTCLGIFIAA
                     IARTESQISGFSTLVLFLAGMLGGAFVPLFLFPGGLANLSRMVPHYWAVQGLYGLFFR
                     GQTLADVWPNAVALLAFAAAFLVIGVRRFKFE"
     gene            15438..16682
                     /locus_tag="AC162G_14575"
     CDS             15438..16682
                     /locus_tag="AC162G_14575"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077080.1"
                     /translation="MDTIRKALAVAVKEIQVFAKDRVWMITMLAVPLAVGAMNAAMQG
                     GGNGDIHLPVIAVNQDAGAYGESIAKAMANIDQIDLRTSSAPAAAEEQVRTGQVLAAV
                     IIPAHFTQRIDDYRPTEITVILDPAQAEFGRIVATIVDEIAGALAIQGEIRYGIKEVL
                     ADTSPDTMADPALARAAQAQTEGVLFTQLQRMETDAPIAVQKETLQGRQVLVWNNVFS
                     IMLPAWTVMFAFFIVPALSTQLLKEKEIGTLRRLLAAPLPRSALIGGKALAFLLIVLL
                     QVVVLFSIGAIFMDMPLGESPLALILTTLALGLTATTLGMMIAALARSISQADSIGLL
                     IVFVLGFLSGALSPQNPPYRGEGLMATVSSLIPQAQAQWAYQTVMFQNGGVTDILPQL
                     TYLFGLSLVFFVIAVWRFRYES"
     gene            16857..>16939
                     /locus_tag="AC162G_14580"
     CDS             16857..>16939
                     /locus_tag="AC162G_14580"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR5077081.1"
                     /translation="MQIHELAQRTGVSAKTIRYYESISLLPS"
BASE COUNT         3152 a         5322 c         5126 g         3339 t
ORIGIN      
        1 tgatcatcat cctggtgctg ctggtccgcc cctagagcct gtccgcaaac cagctcaggg
       61 cgggcgacca gtgcagcgcc agtccaccta ccgtaagcac gccggtcgct ggcgccacga
      121 cgaaatggcc cgatctgcaa tctcgaggct cttacggacg ttctgttcga ttagcaagac
      181 ggccacggcg agttggcttc cctcttctct ctgctcacta gcatggcttg ctcccatacc
      241 gttagagcaa aacgcccggc tcaccaccgg gcgtctgcca tcttcaactt ccacttgagg
      301 accgggctaa tgatgtccgt gcccctccat cggcacgtag cccttgcggt tcgggccgac
      361 gaaatcctcc gggcgctcgt tgaacgtata ccggcagccc ggcgagcaga aatagtacgt
      421 ctcgcccttg tactccgcct tgaacttcgc cgtcttctcg tcgacttcca tgccgcatac
      481 cggatcgatt gccatgcttt cctcctggga agatgtgtcg tgttgtggtg tggagcgtga
      541 aacgtgatca cgttttacgc ttcacgtttt acgccttgac cttcattccc cgcagtctca
      601 gcgaattgct cacgacgaac acgctggaga acgccatcgc cgccgcggcc agcatcggta
      661 tcaaagcgcc gagcgctgcg accgggatca ggatcacgtt gtagaagaac gcccagaaca
      721 ggttctgctt gatcgtgcgc agcgttccgc ggctgagggt gatcgccttg ggcacgccgc
      781 gcaggtcgcc ggacatcagt gtcacgccgg cggcttcgat cgcgatgtcg gtgccggtgc
      841 cgatggcgat gcccacgtcg gcctgcgcca gggcaggcgc gtcgttgatg ccgtcgccga
      901 ccatcgctac aactttgcga aggttcccct cctgcaattg cttcaccgtc gccgccttgt
      961 cgccgggcag cacctcggcc agcgcgcggt cgatcccgac ctcccgcgcg atggcttcgg
     1021 cagtgcgccg gttgtcgccg gtgatcatca cgacttcgat gccctgcgcg tgcagcgagg
     1081 cgatcgcttc tttcgagccg ggcttgaccg tgtcggccac ggcgatcacg ccggccgcct
     1141 cgccgtcgat cgccagcagc atcgccgtct tggcttcggc ttgcagccgc tcgacttccg
     1201 gcgcaagccc gttgagatgc acctgctgct cctgcatcag cgccaggttg cccagcagaa
     1261 cgcgccggcc gtcgactgtc gccgagacgc cgcgcccgga gatcgccctg aacgcctgcg
     1321 gctcggagag cgtcacgctt cgcgtgctcg cagcttcgac gatggcggct gccagcggat
     1381 gctcgctgcc gcgctcgacg ctcgccgcgt aacgcaacag ttgatcagtg gtcagtgacc
     1441 cgtgatcagt ggacactgct gactggtcac tgacaactga taactgatcg ctgatcacta
     1501 cgtccgtcac cttcggctta ccttccgtaa tcgtccccgt cttatccagc gcgatcacct
     1561 gggcattgcc gagccgctcc agtgcttcgc tgctcttgaa caggacgccg ttctccgctc
     1621 ccttgccggt cccgaccatg accgccgtcg gcgtggccag gcccatcgcg cacgggcagg
     1681 cgatgacgag caccgcgacg gcggccttca tcgcaaactc gaatccggcc ccaggattca
     1741 gcagcatatg gccggcgaag gtcaccgccg caatcgcgat caccgccggg acgaacaccg
     1801 ccgagatttg atcggcgagt ctctggatcg gtgctttcga tccctgcgcc tgctcgacca
     1861 tcctgacgat ctgcgcgagc accgtgttct tgccgacctg cgtcgcctcg aacttgagca
     1921 ggccctgctt gttgaccgtc gcgccgacga tcctgtcgcc cgaccccttc tcgaccggca
     1981 tcgattcgcc ggtgatcatc gactcgtcga cgctcgaccg gccctcgatc acgacgccgt
     2041 cgaccgggat cttctcgccg ggacggacga cgaccgtatc cccgacgcgc acgtcgtcga
     2101 tcgacacttc catctccgcg ccgtcgcgca cgatgcgcgc cgtcttcgcg cgcaagccca
     2161 tgagcttctt gatcgcttcg ctggtgcggc ctttggcctt ggcttcgaga tacttgccca
     2221 gcacgatgag cgtgatgatg accgccgccg tctcgaagta catgtggcct tcgaccaggc
     2281 ccaacaggac ggccaggctg tagaagtagg cgacgctcga tcccatcgcc accagcacgt
     2341 ccatgttggc ggcgcggttc ttcagcgcct tccacgcgcc cacgtagaaa tcgcgtccca
     2401 cgtagaactg caccggcgtc gccagcgcca ggaacagcca gcccagcccg ctccagtgga
     2461 agaggggcag caggaactga gggatctcaa gaaagccggc gtgccacgcg tcgttcacca
     2521 tcgacagggc gaacaatgga atcgtgaaga ttaagccgac tgccagccgc cgccgctgat
     2581 gagcgaactc ggcttcgcgc gctttgcgct cggcatcctc acccgacgcg ccttcggcga
     2641 cgatcgcctg gaagcccgcc gcctcgatcg cccgccgcag gtcgccctgg ccgaccatcg
     2701 tcggcaggta ctcgacccgc gccttctccg tcgcgaacga cacactcgcc tggagcacgc
     2761 catcgacctg gctcaaggct tcctggacga ccgccgcatc ccgatcgtcg tgcagcccct
     2821 ggatggggat gtccgcctcg gcgcgtgcga cgccgtaacc gatgtcttcg atccgggcga
     2881 tgacctcttg ctcgctgagc gcgcccggat cgaactcgaa cacgccgcgc tcgctgctca
     2941 ggttcacgtt ggcggcgacg acgccgggca tcttcttcgt cgcccgctcg atcgttgcca
     3001 cgcagttcgc gcaggtcatg ccggtgatag ggagggttac ctgtttagtt gtcatgcttc
     3061 gattccaatg cgtgaaacgt gatgcgtgtt acgtgatacg tgttacgtga tgcgtgatgc
     3121 gtgataacgt aaagccctgt tggtcgtcga tcaccgatca ctggtcactg atcactgatc
     3181 actagatttt ctgcgccatc tcgaacacgt cgacgatctc gtcgagcacc tgctcgcggc
     3241 gtttcttatc gtcgccgcgc acggcgtcga tgacgcacga gttgaggtgc cccttcaaga
     3301 tgaggtcgtt gaccttgttg agcgcggcct gcaccgcctg cacctgcttg atcacgtcga
     3361 tgcagtaggc gtcctggtcg accatgcgct cgatgccggc gacgtggccg gagatgctct
     3421 tgagccgccg cttgagcgtg tctttgcttt gcgcatccat ctttgccctc tcgtctatcc
     3481 cctcccctgg gggaggggat atacacctat tctatcatgc gcacggagcg gcgtcaaggt
     3541 gacgaatgtc acgtgttttc atcggcgtga cgaccccgac gctatcgccg agatttcttc
     3601 gcgcgttttc cgctgcccgt cgccggccgc ttctgcgccg gatacgcctt ctgcaggaac
     3661 tccagcacga ccgggtcgat tcgctcatcg ggctgccagc gctcctcgag attttccacg
     3721 ccaacgagct tctcgaccgc cttgcgcgtg gcatcgtcgt agtcgccgct gaggcggccg
     3781 gtgtaatgcc cgctctgccg ggcgatgcgc tgtaactcgc gcgccacctt tttgtcgatc
     3841 gggatcagcg actcgggatc gggccgcttg aaatacaagt ggtgcagatc gagcagcgcg
     3901 cgcaggcgag cgatcggccg ggagtgatcg tccacgcgca gatcaatgta tcgatcgttg
     3961 aacccggcgt aaccgccctt ctcgcgcacg acgagcagcg ccgcgctctg ctggccgcgc
     4021 ctgtcgccgc cggctttttg cccggccgcc agcgccgcga ccaatcgatc ggccagctca
     4081 cctcgcgcct gctcgaatgc ctggaccatc gccgagacag tccctttgct gacgaggatg
     4141 ttgccctgga cggcatagtg cctgccggtc atgccgcctg cccattcgag gcattccctg
     4201 cccgtgaatg tcgccacatt gccctgcgca tccaccaggc ccacctgccg gtgctcgcgg
     4261 ccttcgtcct cggtcaggag ccggtcgagc gtctcctgcg cgctcaggcc ggacgccatc
     4321 agcgccaatc ctttcggccc gaacgacgtg ttggcatacg actgcgttgc taccgcgccc
     4381 gcgccggcct gcgcccacgg caccgccgag ccgacggcca ggaacttgct ttgcgtcgcc
     4441 acgccccatt cttgcgcatc gggatcgtag gcgacaatcg aaaacgttga aggttggatg
     4501 agtgtgcgca tatcatctcc tgcgaatagc tgatggcaga tagcaaaaca ccttttcttc
     4561 attccacatt cttcattcta cattcttcat tcttccttcc acatttgttc aagaccgctc
     4621 acagcgtctc gtagcgactc ctcgagttcg ggacccgaca cgaactccca ccagcccaga
     4681 tcgtcggcca gtgtctcgac ggcctgcccg tgcagccaga cgccgagccg ggtcttgtcg
     4741 ctcggtcgat cggatcgcca cactttcacc tcgaaacggc tcggcccgcc gcccgaatag
     4801 aaaagcattt gcacgtcgac gagcgccagt gcgccatcct ctaacgcccg ggcgaaccgg
     4861 tacagcccgc tccggatctg gatggcatca tcctggagtg tgtagccgcg ccgcgcgagg
     4921 gcaccgcccg ccgcttgcag cagcatttcc ttgaagatat cgaagggggt ggacacgatc
     4981 ctcaggatgc ttgcataggg tgtgttactt cagctctggc agaggcaccg gctgctcgcg
     5041 cgggtggcat ttgccgcaat acatgacatc ggatacgccg atcgccttct gcgtcccgcc
     5101 ctcgccggcg tacaggccca caatgaaggg tgataggtgg cggataatat acactggaat
     5161 ttttgcgcgt gcaatagccg gttaatctgg tgattgcaca gctctcgctc cccgcgacgt
     5221 cccttcgaca ggctccccac tcccttcggt cggggactac gcagggcggg cctcggcgcg
     5281 ggctgggccg ccgaggactg cggcccaagc atgtgtggat aatcaccaga gctgagtact
     5341 tgccaatgat ggcgtagtgc gctataaaga tactgagcgg ttattcccat gtcaatcgag
     5401 acaggctcac aagccttgag ccaattcgaa cgacgtttgc tggctggaaa tgccaaggcc
     5461 cgcatcaagg ggttattccg tccccatatt gtcatccacg acgcggcgag cgaagtgctg
     5521 ggtgagattc gcgccggact ggtgttcgat cggttccccc accgtgcccg ttgcacggcg
     5581 ctggggatcg agatgtcact ggcggtggcc ggctttctca atcaatcctc tcacttgcta
     5641 gtcaatggag acccaatgtt gcgcgtgagc caccccgcac tctctcgccg ttatgttgca
     5701 gaggactggc aaggtgacac ctatcaactg caccgccgtg gtcgtccatg gcaccgacga
     5761 ttcaacctga tgaggagcgc aggcgaagaa gagatgatct gttttgagtg tgcctcactc
     5821 tgccgtgaga cagtactctc gattgtcgcc caaccggagt tgaagcccgg tgtcattgcc
     5881 ggcttggcgc ttctgctcta ctacaactgg tatctctcgc tgagaactgc cttcatttct
     5941 gccggtggtg caggcggtta gtgtgaacgc gcctaagcta cggtcgatgc ggctcaattc
     6001 atctcagaat tatgcggctt tcacctgttc cgtccgcacg cacgggctag cccgcgccac
     6061 atcaatcctg attcgttggg cggttcgcgc caacgcaagt cgaaaggaga caaagcggtg
     6121 agcgagggcg cggccgaatt ggcgcgcgag cgggtggacg cgatctaccg ctccgactcg
     6181 cgtcgcatct tcgccaccct gatccgcctg ctgggcgact tcgaccttgc ggaggaagcg
     6241 ctgcaggagg ccttcaccgc ggcagtggcg caatggccgc aggctggcgt gccagccaat
     6301 ccccgggcat ggcttgtttc ggttggccgc ttcaaggtca tcgacacact gcgccggagc
     6361 gcccggttcc acgcctcgct ggcggctctc gcggagcaac tcgacgccga cacgaatgat
     6421 actgaggggt gggagggtga gggtgtcgag gacgaccggc tgcggctgat cttcacctgc
     6481 tgtcatccgg cgctgtcgca ggacgcacag gtggcgctga ccttgcgtga ggtgtgcggc
     6541 ctcgcgaccg aggagatcgc gcgcgccttt ctcgttgcgc cgtccaccct ggcccagcgc
     6601 atcgtgcgcg ccaaagcgaa gatccgcgac gcgcggattc cctatgaggt gccgtcgcga
     6661 gcagagttgc ccgaccgact ggacactgtg ctccaagtga tttacctggt gttcaatgag
     6721 gggtactccg cctcatgggg tgagtcgctg acgcgaccgg atctgtcaag cgaagcgatg
     6781 cgcctggggc gtctgctggt tgagttgttg ccggaaccgg aggctttggg gctactggcg
     6841 ttgatgctgc tacacgaatc gcgacgcgcg tcacgtgttt cgccggaggg cgatctggtt
     6901 ttactggagg accaggatcg ctcgctctgg aaccgagaac agatcgctga agggacagca
     6961 ctcgtggagc ggacgctctc gtcgggccgc ctgggccctt acaccctcca ggccgccatc
     7021 gcggtggtgc acgcggcagc gcctgacttc gccgccacgg attgggccca gatcgttagc
     7081 ttgtacgaca tgctggcgca ggtggccccg tcacctgtgg tcgagataaa ccgcgccgtg
     7141 gcggtggcga tgcacgatgg cccgttggcc ggtctggctc tgattgacgc tatcctggcg
     7201 cgcggcgacc tggccaacta tcacttggca cacgcggcgc gagcggatct atgccggcgg
     7261 ctgggaagaa cagcggaagc ccgagcctcc taccaacggg cgctcgccct cacacaacag
     7321 gagccggagc ggcggtttct tgagcgacgg ctaagcaaac tgtcggactg aaccagccaa
     7381 aacccgaatt ttcaaaacca tcgggtcgat tgtcgatttt gggctttccc gaacgactat
     7441 ctaatgtaac agtcattcca cagagccagg tcgagaccgt tcggcggcct ggctcccaac
     7501 cataaggaga aacgaacatg caattcctgg tttacgatcc ccactataac ccggaggcgg
     7561 caactccccc aaccccggag ctattggcag agatgggaga gtttatcggg gaggcaatgc
     7621 aggccggcgt gctggtcacg accggcgccc tccaaccgaa gggcacgcgc ctccggctct
     7681 ccggcgggaa attcaccgtg accgatgggc cgttcatcga gttgaaggag ctaatgggcg
     7741 gctgggcggt gattcggacg aagtcgttag aagaggcgat cgaatggtgc aagcgcttcc
     7801 gcaagatcgt cggggacggt gagactgaga tcgttcaggt gttcgggccc gaagactcac
     7861 ctgcctagct ctttcctctc ctgaagctcg cgcagaacgt tctggtctct gcgcgagcaa
     7921 cggccggagt cgggcgtccc ggacttctcg ggcgcccgcc tcgtgtccac cgcaaagtat
     7981 cgtgcgatca atctgctgcg ccgaaacaag caggttgagc gcaagcacga gaagatcgtt
     8041 gtcgccccaa cgtcatctca cgtccacatc gcgccatcca gtgcactctt gtcagggcaa
     8101 gcgtcgcgct caagccacag gcgccagaac tgcgatgtct caagcaacgg ctcggccagg
     8161 tgctggactc aatcggcgaa tgcaggtttg tcaagtttgg caggccgatt tgtcgatttt
     8221 ggactccact gaacgattac ttcatgaaac agccagttca acggatgggg actcgaaagg
     8281 agatgagatg atgaagacta tgaacaaact aacgctcacc gcgttcgcaa tgatctgtgc
     8341 ggtcacgctc gtcgtgggat gtgcaccgcg caacaacacc gataaaggag atgaaaggat
     8401 gagcaccacg acaacgaata agccagcgct caccgccggc aactacgctt ccgtgaatgg
     8461 tctcaacatg tactacgaga ttcatggcgc gggagaaccg ctgatcctgc tgcatggtgg
     8521 cgtcggcgca atcgaaatgt tcggcgaggt cctgccgctg ctcgcagagg gccggcaagt
     8581 catcgctgtc gatttgcagg cgcatgggcg cacggccgac atcgaccgcc cgctgagctt
     8641 cgcgttgatg gcggacgaca tcgccgccct gatcaagcat ctcggcctcc aaaaagccga
     8701 tgtcatgggt tactccctgg gcggcggcgt cgccctgcaa gtcgccatcc ggcatccgaa
     8761 cgcggtgcgc aagctggtgg tcgtgtcgac cccattcaag cgggatggat ggtaccccga
     8821 ggtccttgcg ggaatggagc agatgggtcc tgaggccgcg gagccgatga agcagacccc
     8881 gatgtaccag ctctatgcca gggtcgcgcc gaagccggaa gattggccgg tgttgctcac
     8941 caagcttggt gagctgctgc ggcagggcta tgactggtcg gaggatgtgg cggcgattaa
     9001 ggcgcccacg ctgatcgtgg ttggggatgc cgacagcgtc cgtacttcac acgctgttga
     9061 gctatttgaa ctgctcggcg gcggcaaggc cgacgccgga tgggacggag ccggaatgtc
     9121 caatgcccgg cttgccatcc tgccctccac aacgcattac aacatcttct cctcccccgc
     9181 gttggcgtcc accgtcacgc cgttcctaaa cgcgccgatg tcccaggctc agtgagccgc
     9241 ccgcctatat agcgcttcat tgtgcgatac cacctgagga tcgatctggc gaagcgcgaa
     9301 accgccgagc gtgtgctggg atttcatgca gactactgac cggttgcatg cagcatcggc
     9361 ggctgcgtgg cgatcatgac ttcgctggag atggcggatg cctgagtcgg caagctgata
     9421 agaatcttga gtggagggat gtgttgaggt agaatagaca gggtggtcta cggacctcgc
     9481 atgcggcgag gtatggttcg cgtctcatcg ttgcgctcca cggagtattt tcgagatgac
     9541 gacgccactc ctgacgacca agctccacat tccaccgctg ctcccggatc gggtgcctcg
     9601 tctgcggctg atccgtcgac tggacgaggg gctgcggtca gggaacaagc tgacgctcgt
     9661 ctcggccccg gccggctatg gcaagacgac cctggtcgca gagtggctga ggcacctgca
     9721 ggatgcagac gacgcggcgc tggccgtctc ctggctttca cttgacgaag gggacagaga
     9781 cccggctcgc ttttgggcct atctcgttgc ggcactgcag gcagtcgatc cggccattgg
     9841 ccagaccgca caggtcatgc tgcatagccc tcagcctcct cctcccgagt cgtttctaac
     9901 agccctgatc aacgagatcg ccgccatccg ccggtcgttc gtcctcgtcc tggatgatta
     9961 ccaccttgtc gaagccttgc ccattcacca acagttgacg tttctcatcg agcatcagcc
    10021 cactcaaatg cacctcgtga tcgctacccg cgaggacccg ccgctgcccc tgtcgcgcct
    10081 gcgtgcacgc gggcaaacag tggaaattcg gcaggacgat ctgcgctttt cgcgcgaaga
    10141 gtgcgccgat ttcctcaaat cggtgatggc gcttgacctc tctcaagacg atatcgccgc
    10201 cctggagcgt cgcacggaag gatggatcgc cggcctgcaa atggcggccc tttcgctgca
    10261 acataccacc gattctagag gtttcgtgca gtctttcacc ggaagccacc gctacatcct
    10321 cgactacctg atcgaagagg tgctcagcaa acaaccatcc catcttcagg attttctcct
    10381 caagaccgct attctcgatc gactgtctgc gtccttatgc gacacagttg ccgagcggag
    10441 tgatagccat gaagtgctcc aacatctgga agcggccaat gttttcattg tgccgctcga
    10501 tcccggccgg gactggtacc gctatcacca cctgttcaca gagctgctgc gccacagatt
    10561 gcgactggct cctggaatca ccgaagagac actgcaccaa agagccagct attggttcga
    10621 gcaaaaccaa tatccattcg aggccgtgca gcacgcgctg gcggctgcgg attgggagcg
    10681 atctgcacaa ttgatccata ccgagtccga tggcatgctt aagcgcggcg aagtcgccac
    10741 cctgctcggc tggctgaaga aactgcctga agaacaggtg gccgcgcgtc cagggctttc
    10801 tcttaccttt agctggccgc tcatcctgag cggacagttg gaaacagccg agcggttgct
    10861 gacacatgca gagcagggag ccggtaagga ccctcatgtt ctcgggcaga tcgccgctgc
    10921 gcaggcttat attgcccgtt cgcgtgggga tgatcagcgc acggtggcga tgtcacagcg
    10981 ggcgctgtcc ttgctgccgg aaacggacgt agcattgcgg gagctattgg ccgtcaacct
    11041 gggaatcgcc cactggcaca acgggcagct cgaagaagcg gacaatgtgc tgaacgaggc
    11101 atggcgactc tcagttggat tggggaatcg ctatgcagaa atgacgatcc aggttttttt
    11161 gaacagaatt ctggcggcac gcggattgct gcagcaagct tatcaggcat atcaacccct
    11221 ggtcgagttg gaagatcagg tgcctatcct agcgctggct catctggacc tgagcgcgct
    11281 gcgctatgaa tggaacgacc tggaagcctg cgccactcac atcgaaaaaa gcatcgccgt
    11341 gagcgaacgt acccggaatg gagagtttca gatttctgga cacatccaga tggcccgcct
    11401 caggttggca tacggagacg cgaatggcgc agcgacagcg ctacgacatg cggaacagct
    11461 tctacaggca cacagcgtga cgccactgac gagagcgcgc aacgcagcgg cacacgtgca
    11521 ggttgccctg ttccagggtg acctgtctgg cgcatcggcc tgggcacgag atgcgggtga
    11581 tcacgccgat gtccatcctt tctatccgtt tctgggtttg agccgggcgc gcctgctgct
    11641 tgctcaaaac cgccaagcgg aagccctgga ttgtctgaaa gaccgctttg aaatcgccgc
    11701 gcgagctggc tggagctacg gccagatcgc cgtgcgtgtg ctgcaggctg tggctgccgg
    11761 tcagtccgag gaagcgctcg attttttgat ccaggccctg acctgggccc agccgggtgg
    11821 gttcatccgc acttttgtgg acgccggcgg cgcgctggcg ccactgctgg aagaagccgc
    11881 cctgcgcgga gtgatgccgg aatacgtagg gacgctgctg gccgctctcg gaagccaggc
    11941 gaaggttctg caactgacca aagccacggg cgtgtctccg ctggttgaac cgttgagcga
    12001 acgcgagatc gaggtgctgc ggctggtggc ggccggtctt tcgaaccggg aaattgcgaa
    12061 aaaactggtc atcagcccgg ggacagccaa gacccatatc cacaacctgt gcgggaaact
    12121 cggcgtccgc aatcgcacag aagcggcaac gcgcgccaaa gaactgggtc tggtctgaga
    12181 cacaaaatgc cagatcctgc agccatccgt tgaggatggc tgttttgttt gcaataaccc
    12241 cccagataaa ccccttgaat ctgccagtcg gtagatgaca tttcgtgccc ggaaaagtat
    12301 agtccaatga gaaggaggca aaaatgaaca agtcacgact catctggggg atcatctgtc
    12361 tgatcctggc ggcggcgctc ggcgtgctgg acattgtcct gccgcctgac agactgatgt
    12421 tccaggtcgg cgatcagaac atgtactgga ttccggcgat cgttttaggg atcgtcggca
    12481 tcgtgctgct ggccaccgct ggaagcagcg ggcaagcaac tgctgaggcg aagcaaccgg
    12541 cggtcgttca aaatcccgaa aaggccgcct taaacaaacg cctggagaac atcggctggg
    12601 ggctgttcct gatcatgctg ggcgggtttg ctctcgtccc cgacgaatcg gttcccaaag
    12661 gactgtggtc gatcggcgtc ggggtcatca tgctcgggct gaacgtgacg cgctacttct
    12721 acaagatcaa gatgagcggc ttcaccacca tcctgggcat tcttgccctg cttggcggca
    12781 tcgccgaatt gctcggattc acgtcgctgg aaggagggct cctgctcatc atcctcggcg
    12841 cctacttgct cctcaagccg tggttcgaga aacggcagct cttcggcaag gctgaggaaa
    12901 gctaatttcc ggagagagag ccaatgctga aagtcgagat ccaggttaag ggatgtattg
    12961 acaaggattg gtcagattgg tttgcgggtc ttgcgatcgc ccatccggag gaaaactaca
    13021 cggtgctgac cggctctctc ccggaccagg ctgccctgtt cggtctgctc acacagatac
    13081 gaaacctggg cttgccgctc gtatcggtga gcagtgaaga acaaaaccag actgaagacg
    13141 tatgaaggag caatacccat gcccgctatt ctggaagtcg ataacctcgt gaagaagtac
    13201 ggcgactttg aagccgtcaa agggatcagc ttctccgtcg aagagggtga ggtctttggc
    13261 ctgttagggc ccaacggcgc cggtaagacg cagacgattt ccatgctgac cggcgtgatt
    13321 cagcccacat cgggcacggc gcgcatcggc ggctacgaca tccgcacgcg gatgggtgag
    13381 gtcaagaaga tcaacggcct ggtgccccag gacctggcgc tgtaccccac gttgagcgcc
    13441 cgggccaacc ttaacttctt cgggcgcatc tacggcctga gagggaaaca gctcaaggag
    13501 cgggtggacg acgtgttgcg cgtcgtggcg ctggccgagc gcgccgacca ggcagtggac
    13561 aaatactccg gcgggatgaa gcgccgggtc aacatcggcg ccggcctgct ccaccatccg
    13621 cgactgttct tcctcgacga gccgaccgtc ggcgtcgatc cgcagagccg caaccagatc
    13681 ttcgagagcg tgcagcagct caaccgcgag cgcggcatga gcatcgtcta taccagccac
    13741 tacatggaag aagtcgagct gctgtgcaac cgcgtggcga tcatcgacca tggccaaatc
    13801 atcgccatgg acacgatcaa gaacctgatc gccatgctgg gcggcggcgt gatccatctc
    13861 ggcctgagcc gcgtcgacga tgcgctgctg aaagatctgt ccgccttgcc gggcgtcaag
    13921 cggacgagcc tggcgccgct gccggtcgcg ccggcgaatg gcgaggccgc gccgcaagcg
    13981 aagggaccga tcgtgaagat cgagtgcgag aacagccagc gggcgctggt cagcgtggtc
    14041 tcgtacctcg gcgggcatgg gctgggcgtc acgtcgctgg agacgctgga acccaacctg
    14101 gagagcgtgt tcttgcatct caccggcaag agcctgcggg agtgagtgcc atgaacctgc
    14161 tcagcgtcac cctaaaggat ctccaagtct tcgtgagaga ccgcggagct gtgtttctgc
    14221 tcttcctgct gcccttcgcc ttcatcgccg tgctctccct ggctacgcaa ggagttcgac
    14281 tgggcgagag cggcgacggc ggtcaaaccc gcgttccgct gaccgtggtg aacaacgatg
    14341 cgcagggcca ggccgcacag gaatttctgg ccgcgctgaa cgacactggc aaggtacaaa
    14401 tcgatctggg aggtccgcag gcgaaagtcg aacggcagct caacgatgcg acgctgcgct
    14461 acgcgctgtt catcccggtc gatttcagcg ccaaactggc tgcaggcgat caagtcagcc
    14521 tgcgcctggc gctacacccc tacgctaatc aggccgacct gctgaccgtg gaacgagcga
    14581 tcgtccgcgc ggctcgggaa tatctcataa tggggtatct gaacgacggc ctggagcaga
    14641 tggcggccat gcaggccgcc aatcctcagg cggcgtcgac cttctcaaag gagcgtatcc
    14701 agcagcagat tgaagcgcaa caggctcagg cagagcagcg gcccttgatc acggtaacga
    14761 caaccactcc ggccgtgaag ggcgaaggac aggcagcggc agaagagaat cttccaacat
    14821 acggccaatt cctcgtggtg ggcatggccg tcctgttcgt cttcctcgcc gcgcagaaca
    14881 cggcccgctc gatctacgag gagaagcgcg agggaacctt ccgccgcctg atggccgcgc
    14941 cgatcagcaa ggcttcgctg ctgggcggca agctcctacc caacttcctt ctagtcctgg
    15001 tgcaggtcgc ggtcatcctc gtcaccggcg gtttcgtgat ccaactgttc ggtgtgccac
    15061 cgctgaacct cggctcggac ttgctggggc tggttgtgat atgtgcggcc acggctctat
    15121 gttcgacctg tctgggcatc ttcatcgctg cgatcgcccg gacggaaagc cagatcagcg
    15181 gattctccac gctggtcttg ttcctggccg gtatgctggg cggcgctttc gtgccgctgt
    15241 tcctgtttcc gggcggcctg gccaacctgt ccaggatggt gccgcactac tgggctgtcc
    15301 agggacttta cggtctgttc ttccgcggtc aaacgctggc ggatgtctgg cccaacgccg
    15361 tggccctgct ggccttcgcc gcagcgttcc tcgtcatcgg cgtgcggcga ttcaagtttg
    15421 agtagaggag caggatcatg gacacgatcc gcaaagcact ggccgtggct gtcaaagaga
    15481 ttcaggtctt cgccaaagat cgcgtctgga tgatcaccat gctggccgtc ccactggcgg
    15541 taggggccat gaatgccgcg atgcagggcg gtggtaacgg cgacatccat ctgccggtca
    15601 tcgcagtcaa tcaggatgcg ggcgcctacg gtgagtcgat cgccaaggct atggcaaaca
    15661 tcgaccagat cgatctgcgg acatcgagcg caccggcggc agccgaggag caggtgcgca
    15721 ccggacaggt gctggcggcg gtgatcatcc cggcgcattt cactcagcgc atcgacgact
    15781 accggccaac cgagatcacg gtcatcctgg acccggctca ggcagaattc gggcggattg
    15841 tcgccactat cgtggatgag atcgccgggg cgttggccat tcaaggcgag attcgatacg
    15901 gaatcaaaga ggtgctggcc gacacaagcc ccgacaccat ggctgacccg gctctggccc
    15961 gcgccgccca ggctcagact gaaggcgttc tgttcaccca actgcagcgc atggagacgg
    16021 atgctccgat cgcggtgcag aaggaaaccc tacagggccg ccaggttctg gtatggaaca
    16081 atgtcttcag catcatgctg cccgcttgga cggtcatgtt tgccttcttc atcgtgccgg
    16141 ctctgtccac tcaactgctg aaggagaaag agatcggcac actgcgccgc ctgctggccg
    16201 ctcccctgcc acgcagcgcg ctgatcggcg gtaaggcgct ggctttcctg ttgatcgtcc
    16261 tccttcaagt ggtcgtgctc ttcagcattg gcgcgatctt catggacatg ccgctgggcg
    16321 aatcgcccct ggctttgatc ttgaccacac tcgccctggg gttgacggcc acgacgctgg
    16381 gcatgatgat cgccgccctg gcgcgctcga tcagccaggc ggactcgatc ggtctgctga
    16441 tcgtcttcgt gctgggtttc ctgagtggcg cgttgtcgcc gcagaacccg ccgtatcgcg
    16501 gcgaaggcct gatggcgacc gtctccagcc tcatcccgca ggcgcaggcg caatgggcct
    16561 atcagaccgt gatgttccag aacggcggcg tgaccgacat cctgccgcaa ctgacttacc
    16621 tgttcggcct gtcgctggtc ttcttcgtga tcgccgtgtg gcgcttccga tacgagtcgt
    16681 gacggctcgg ggtatagccc acccaccacg tcgagattcg agtgaagcgg atagaccgct
    16741 ccagccccaa gcgtaagaca cgcttggggc cgcgtcattc ctgacattcg atcttgacat
    16801 tccagtgact ggaaggtgta caatgcgctc acactcagca ttcgacgagg ctcgctatgc
    16861 agattcacga gttggctcag cgcacgggcg tttccgcgaa gaccattcgc tactacgagt
    16921 caatcagcct cttgccctc
//
LOCUS       DBNHXB010000357         4446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2447089, whole genome shotgun sequence.
ACCESSION   DBNHXB010000357 DBNHXB010000000
VERSION     DBNHXB010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2447089"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="AC162G_14585"
     CDS             <1..1145
                     /locus_tag="AC162G_14585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5077082.1"
                     /translation="REWKAPPQERLPWALLRAADKLGLRSLGVPEKYGGIKLEAQAQT
                     FALIAEELARGDSGFADIMGQIWKVPVLLSHIAPPHLQDVWFPRFMEDPTFLMAHCLT
                     EPRGASDRWLPYNAPEANMHTKAVFKGGHWVINGRKQFITNGYDAKLYVVYANTNPNV
                     GVADGTSSFLVPRDTPGLIPTRVNEKIGARYMNNGEIVFEDCRVPEDHLLVKDVAIKK
                     AGVYFSPGKILQAAKNLGVGVAAYEETAAYVQQYVQGGRTLIKHQAVAKAVADMAIQL
                     ETVRAFTRYAARALDEGASDAGTLTLMAKVYAADAVFDVCKTAMELHGGSGVMIEVGI
                     EKHFRDAAVFLHMDGTQDIHRFKIVRSMFPDTAGMYAGPEESLLIQ"
     gene            1228..1905
                     /locus_tag="AC162G_14590"
     CDS             1228..1905
                     /locus_tag="AC162G_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HIR5077083.1"
                     /translation="MLMVSEAWRSAFPGAVAGTLVMRNVANPARHPALDARKEELENQ
                     LRARFSGQDRATLKALAPVQAYNAYFKRFGKTYHVLLQVESVALKGKPIPRVSALVEA
                     MFMAELKNLLLTAGHDLESVQPPVRLDVSDGSERYVMLNGREQVLAPGDMRMADTQGV
                     ISSVLYGPDHRTRITLDTRRVFFAVYAPPGIGEQAVAHHLQDIQSSVMMVSPEAEVEA
                     LSVYRAE"
     gene            1916..2974
                     /locus_tag="AC162G_14595"
     CDS             1916..2974
                     /locus_tag="AC162G_14595"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5077084.1"
                     /translation="MPDERVQAAIDNWAPRFIAQGVDYNDFFRTTARVERWDDWCREW
                     CATGDVHANLAAAAAAKGNMVSAGEAYIAAALCYHFAKFLFQDHHDEYVAAGRKSIDA
                     FAKGLRLLDPSVERVGIPFDGATMVGTFRRPPGSDRPPLILLLPGLDSVKEEFFYWEN
                     VFLKRGLATFSLEGPGQGECGYHTHIRPDYEVAVSTALDVLAKRDDIDPKRIGLAGVS
                     LGGYYAPRAAAFEPRVKAAVGNCGPWNFAECWSHLPSLTRAAFQHHSGAKNGAEALAN
                     AGKLNLDGVAQKIKQPLLIIHGKLDRLIPWQQAHKIVDAVGPNAELAMFENGNHVCNN
                     IPYIYRPLTADWLKEKLG"
     gene            complement(2954..3220)
                     /locus_tag="AC162G_14600"
     CDS             complement(2954..3220)
                     /locus_tag="AC162G_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077085.1"
                     /translation="MTILSAPDQPISTLTVAELETLITEIVRRVLREEMLQSVRENGR
                     TMSEEFLATFGAWEDDRSADEIVAEIYASRTTSSPKGPSPQFLL"
     gene            3278..>4446
                     /locus_tag="AC162G_14605"
     CDS             3278..>4446
                     /locus_tag="AC162G_14605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR5077086.1"
                     /translation="MTKDTYPRFSDSEFQRRNGLVRDLMRREGLAALILFGNSGVNRH
                     NQANVFWLTNYLDLHHNYLVFPLDGEPLLLTGLANHVPNARLISVIPDTRWGGYEPPR
                     EMVAHLKKSGIGTGRVGLVGVNATFGMGMPYQHFLDLREGLPEVEWVDVTRDFARLRA
                     VKSEEEFAWLEKAAEFTDMTLAALEAETRPGLTEYDLIGIVEGAYRRKGGQPHIAFLR
                     SMPMNAPTACVPAQNVTGRVIQKGDVIITEISASFWGYSGQIHRPIAVSQDPAPEWRR
                     LFEAGVQAYERMCAVIRPGANEADVIKAASIIGESGYRIYDDLIHGYGVDIHPPMIDR
                     SCCDYWPWDDTRPAPAGRAFAEDMAIVVQPNPITPDERMGLQVGALTRVTKNGAV"
BASE COUNT          866 a         1364 c         1406 g          810 t
ORIGIN      
        1 aaagagagtg gaaggccccg ccgcaggagc ggcttccctg ggcgctcctc cgtgcggcgg
       61 acaagttggg gctgcgcagc ctgggcgtgc cggagaagta cggcgggatc aagttggagg
      121 cgcaggccca gactttcgcc ctcatcgccg aagagctggc ccgcggcgac tccggcttcg
      181 cggacatcat gggccaaatc tggaaggtgc ccgtgttgct cagccacatt gctccgccgc
      241 acctccaaga tgtgtggttc ccgcgcttca tggaagaccc cacgttcctg atggcgcact
      301 gcctgactga gccgcgtggc gcttccgacc gctggctgcc gtacaacgcg cccgaggcca
      361 acatgcacac caaagccgtt ttcaagggcg gacactgggt gatcaacggc cgcaagcagt
      421 tcatcaccaa cggctacgac gccaagctgt atgtggtcta tgccaacacc aaccccaacg
      481 tgggcgtcgc agacggcacg agcagtttcc tggtgccgcg cgacacgccg ggtctcatcc
      541 ccacccgcgt caacgagaag ataggggcgc gctacatgaa taacggggag atcgtctttg
      601 aggattgtcg cgtgccggag gatcatttgc tggtcaagga tgtggccatt aagaaggccg
      661 gggtctactt cagccccggc aaaatccttc aggcggcgaa gaacctgggc gtgggtgtgg
      721 ccgcctacga ggaaaccgcg gcctacgttc agcagtacgt acagggcggc cgcacgctca
      781 tcaaacacca ggccgtggct aaagctgtgg ccgacatggc catccaactg gagacggtgc
      841 gcgccttcac ccgatatgcc gcccgagcgc tggacgaagg ggcctcggac gccggcacgt
      901 taaccctcat ggctaaagtc tatgccgcgg acgcggtttt cgacgtgtgc aagacggcca
      961 tggaactcca cggcggcagc ggcgtcatga tcgaagtcgg gatcgaaaag cacttccgcg
     1021 atgccgccgt cttcctgcac atggatggca cgcaggatat ccaccgcttc aagattgtga
     1081 ggtcaatgtt ccccgacaca gcggggatgt atgcaggacc tgaagagagc ctgctcatcc
     1141 agtaggccga tcggatccgt gcgcattggc cttgatgcgg tgattgcgaa gtagctttgc
     1201 tcggccggga aggagacccc gttagcgatg ctcatggtat ccgaggcgtg gcgaagcgcg
     1261 tttcccggag cagtggcagg gacactcgtc atgcggaacg tcgccaaccc ggcccgccac
     1321 cctgcgcttg acgcgcgcaa agaggaactg gagaaccagc tacgggcgcg tttttccggc
     1381 caggatcgag ccacactcaa ggcactggcg cctgttcagg catacaacgc ttatttcaag
     1441 cgtttcggaa agacgtatca cgtcctgctg caagtggaat cagtcgccct caagggcaag
     1501 ccgattcctc gagtctcggc gttggtcgaa gcgatgttta tggccgaact caagaacctg
     1561 ctcttgacgg cagggcacga tctggaatcg gtgcagcctc ccgttcggct cgatgtgtcg
     1621 gacggcagcg agcgatacgt gatgctcaat ggacgtgagc aagtgctcgc gcctggcgac
     1681 atgaggatgg ccgacacgca gggtgtcatc tccagtgtac tctacgggcc tgaccatcgc
     1741 acgcgaatca cactggacac ccgccgggtc ttctttgcag tctatgcgcc gccgggcatc
     1801 ggcgagcagg cggtggccca ccatctgcag gacattcagt cgagcgtgat gatggtctcg
     1861 cccgaagccg aggtcgaagc gttgagcgtg tatcgcgccg aatgagggag agacgatgcc
     1921 cgacgaacga gtgcaagccg cgatcgacaa ctgggctccg cgtttcatcg cgcagggcgt
     1981 ggactacaat gacttcttcc gcacgacggc ccgcgtcgag aggtgggacg attggtgccg
     2041 tgagtggtgc gcgacgggcg acgttcacgc caatctcgcc gccgcagccg cagcaaaggg
     2101 caacatggtg agcgccggcg aggcgtacat cgccgccgcg ctgtgctatc acttcgccaa
     2161 gtttctattc caggatcatc acgacgaata cgtggcggcc gggcgcaagt cgatcgatgc
     2221 cttcgcgaag gggctgcgcc tgctcgatcc aagcgtcgag cgcgtgggga ttccgttcga
     2281 cggcgcgacg atggtgggca ccttccgccg cccgcccggt tcggatcgac caccgctgat
     2341 cctgctgctg cccggactcg attccgttaa ggaagagttc ttctactggg agaatgtctt
     2401 cctcaagcgc ggcctggcga ccttctcgct cgaaggccct ggtcagggcg agtgcggcta
     2461 tcacacgcac attcggccgg actacgaagt cgccgtatcg acggcgctcg acgtgctggc
     2521 caaacgcgac gatatcgatc cgaagcggat cggcctggcc ggtgtgagcc tgggcggata
     2581 ctatgcgccg cgcgcggctg cgttcgagcc gcgcgtgaag gcggcagtgg gcaattgtgg
     2641 gccgtggaat ttcgccgagt gctggtcgca tcttccgtcg ctgacacgtg ccgcgttcca
     2701 gcaccattcc ggcgcgaaga atggagctga ggcgctcgcc aatgccggca agctcaacct
     2761 cgacggcgtc gcgcagaaga tcaagcagcc actgctcatc atccacggca agctcgatcg
     2821 actgatcccc tggcagcagg cgcacaagat cgtcgacgcc gtcggcccga acgcggaact
     2881 ggcgatgttc gagaatggga accatgtatg caacaacatc ccatacatct atcgtccgct
     2941 gacggcagat tggttaaagg agaaactggg gtgaagggcc tttcggcgag gaggtagtcc
     3001 ggctcgcgta aatctcggcc acgatttcat cggcggagcg atcatcttcc cacgcgccga
     3061 acgtggcgag aaactcttcg gacatggttc gtccattctc gcgaacggat tgaagcattt
     3121 cttcgcggag gacgcgacgg acgatctccg tgatgagcgt ctccaactcg gcaaccgtga
     3181 gggtgctgat cggttgatca ggagcggaaa gtatagtcat ctcacacctc ccagaggttt
     3241 caaacctcgt gtctatcata taccattata taggcaaatg accaaagaca cctacccacg
     3301 tttttccgac tcagaattcc agcgccgcaa cggcctcgtc cgcgacctta tgcgccgcga
     3361 agggctggcg gcgttgatcc tcttcggcaa ctccggcgtt aatcgccaca atcaggccaa
     3421 cgtcttctgg ctgacgaact acctcgatct gcatcacaat tatctcgtct tcccgctcga
     3481 tggcgagccg ctgttgctca ccggccttgc caatcacgtt cctaatgcgc ggctgatttc
     3541 ggtgattcct gatacgcgct ggggtggcta cgaaccgccg cgcgagatgg ttgcccatct
     3601 caagaagtcc ggcatcggca caggccgcgt cgggttggtc ggcgtcaacg cgacgtttgg
     3661 aatggggatg ccttatcagc acttcctcga cttgcgcgag gggctgccgg aggtcgagtg
     3721 ggtggacgtg acgcgcgact tcgcccgcct gcgcgcagtg aagagcgaag aagaattcgc
     3781 gtggctcgag aaggccgccg agttcaccga tatgacgctc gccgcgctgg aggccgagac
     3841 gcgccccggc ctcaccgagt acgacttgat cggcattgtc gagggtgcgt atcggcgcaa
     3901 gggcggccag ccgcacattg ccttcctgcg ctcgatgccg atgaacgcgc cgacggcgtg
     3961 cgtcccggcg cagaacgtca ccggccgcgt gatccagaaa ggcgacgtga tcattaccga
     4021 gatcagcgcc agtttctggg gctattccgg tcagattcac cgcccgatcg cggtcagcca
     4081 ggacccggcg ccggagtggc ggcggctgtt tgaggccggc gtacaggctt acgagcgcat
     4141 gtgcgccgtg attcgtccgg gcgcaaacga agccgacgtg atcaaagccg cctcgatcat
     4201 cggcgagtcg ggctaccgca tctacgacga tttgattcac ggctacggcg tagacattca
     4261 tccgccgatg atcgatcgct cgtgctgcga ttactggccg tgggacgata cccggcccgc
     4321 gcctgctggc cgagccttcg ccgaagacat ggcgattgtc gtccagccca acccgatcac
     4381 gcccgacgag cgcatgggcc tgcaggtcgg cgcactgacg cgggtgacga aaaacggcgc
     4441 ggtcag
//
LOCUS       DBNHXB010000358         5321 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1775228, whole genome shotgun sequence.
ACCESSION   DBNHXB010000358 DBNHXB010000000
VERSION     DBNHXB010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1775228"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="AC162G_14610"
     CDS             complement(<1..343)
                     /locus_tag="AC162G_14610"
                     /inference="COORDINATES: protein motif:HMM:NF012343.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR5077087.1"
                     /translation="MWSDQPGSASRRFFYSALFWLIVPGIFGLLLATALYVPSVYDTL
                     PLAIKPYLSFGRLRPTHVNLMIFGWLSQAYVGAILYILPRLTRARLYSERLAHVNWWL
                     WNLMIAGAAITL"
     gene            complement(439..1077)
                     /locus_tag="AC162G_14615"
     CDS             complement(439..1077)
                     /locus_tag="AC162G_14615"
                     /inference="COORDINATES: protein motif:HMM:NF014672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR5077088.1"
                     /translation="MATPRADRTLDCRGLMYPVPILKAEQALLEMPAGQVLEVMADDP
                     NTKPDLAKWAERIASELVTISDTANGQYSFFVRKGARQAASPQLAAKDENLFLVVLRS
                     GLNAPGQVRAAFMYASLAAAMGQDTVVYCVQEGADAAKKDVSQKDTSPAGGPTISQRI
                     AEALDMGVRIEVCEQTATVRSIKAEDLIPEAVLIGGASLIDYAIRARGQLTF"
     gene            1271..1828
                     /locus_tag="AC162G_14620"
     CDS             1271..1828
                     /locus_tag="AC162G_14620"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012982.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HIR5077089.1"
                     /translation="MSSDSVTLFHRAFDKLYPSIRFVYERIQGHAWFDRITPRGKVTA
                     ELWMGGAPTYRRDYEFILQHGITAVVNIRSERADDTDFYDAHGITHVRYRVPDITVPD
                     EKVISDGVDWIKQQVDDGRAVLIHCAKGRGRSATLLAAYLMREEGMTYDEAWQLVKSK
                     RRLAKLETRHRERLEAWIARNGRQA"
     gene            1922..2590
                     /locus_tag="AC162G_14625"
     CDS             1922..2590
                     /locus_tag="AC162G_14625"
                     /inference="COORDINATES: protein motif:HMM:NF046753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6989 domain-containing protein"
                     /protein_id="HIR5077090.1"
                     /translation="MKRLATFRTEDRWIIGSMALTLGGVMLGAILAEPRAFGITAVVV
                     ICLLLIAWYKTRSPKLAWLLVFGFIAGLLELWADWVHVTYLKSLIYTDYFGFNLLASP
                     SYMPIGWWLTVVQFGYLGLRLTDRWPIWKAVGLLTLLGMTLPPWYEEFAAPARAWHYS
                     PQGIMLSNTPLWIILTYGGCMFAIGSAAVLLYSPRAWGRAVLGGIFTGASLMFSGLFW
                     FSVF"
     gene            2821..5169
                     /locus_tag="AC162G_14630"
     CDS             2821..5169
                     /locus_tag="AC162G_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008736257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HIR5077091.1"
                     /translation="MDRSSLHRQLNILLVALLLLALALGLAPSVAGATSGDNPAGEGF
                     TVRVVSSAPDQVTGGDARLHIVVPLIVPLRRVGVWVNGVDQRSHFSALPGTRILTGVI
                     DGLAIGENRLVVKADGRGERRPAPVAMTLTNYPITGPVFSGPHQHPFVCTVMTEGLGQ
                     PIPDHPSTGTKVFDASGSVIGYSRDCSVPTQVVFKYRTTDNTWKDYTPGMPRPSDMAQ
                     TTTLDGLTVDFIVRWERGTINRFIYSIAVLAPFDDDAWELNRGAWNKRVIYRFDGGVA
                     IGHTQGRISTSSMLYDVGLARGYAILYSTGTRTSTHYNLQLGGETALMVKERFVELYD
                     VPLYTVGVGGSGGAIQQYVYAQNHPGLLDAAIPQYSYPDMVTQAVHIGDCELLEFYMD
                     VLDGVNPRWKTWTNRSLLEGLAASDTLPNPYNLGKPGLTECINGWRGLSPLALNPLYG
                     LAPNQQFYEPPSAIAAIEWTHFADIVNIVGRDADGYARRYWDNVGVQYGLQAVASGQI
                     TPDEFLKLNAMVGGWKEEADMVQEGSPFYPPGVIDPANWDPWSQRNQVYSLDPLNNPA
                     PRTAGSLEAMQAVYEAGLVFTGDIDIPVIDWRHYLEDELDMHNSHQSFASRQRMLNAD
                     GDASNQVIWFTDVVVSSQRFDQTPEALEVMDEWILNILAHPDLGVAGNKPPRAADRCF
                     DDKGVEIASGPDVWDGILNDDPPGACTQRFKLYSTSRRVAGGPFEQSIFKCQLIPVGE
                     AVARGFYGVWMPDAAQTMLLQRIFSHGVCDYSRPDQGLPPGH"
BASE COUNT         1006 a         1640 c         1644 g         1031 t
ORIGIN      
        1 gcagcgtgat cgccgcgccc gcgatcatca ggttccacag ccaccagttg acgtgggcca
       61 ggcgttcgct atacagtcgc gcccgcgtga ggcgcggcag aatgtacagt atcgcgccga
      121 cgtaggcctg cgacagccag ccgaagatca tgaggttgac gtgggtaggg cgcaggcggc
      181 cgaaactcag ataaggtttg atcgccagag gaagagtatc gtagacggag ggcacgtaga
      241 gagccgtcgc cagaagcagc ccgaagattc caggcacgat cagccagaag agggccgagt
      301 agaaaaagcg gcggctggcg ctaccgggtt ggtcgctcca catgtttcgc atccaatgag
      361 actcgataga tccaaatttg gacggcgagc aagacttcgg aagtctcaaa agacttccga
      421 agtctgaaaa tcgagacttc agaacgtcag ttgtccacgc gcgcggatgg catagtcgat
      481 caacgaggca ccgccgatca gaacggcttc cgggatcagg tcttcggctt tgatgctgcg
      541 aacggtggcc gtctgctcgc agacctcgat ccggacgccc atgtccaatg cctcggcgat
      601 gcgctgagag atcgtcgggc cgcccgccgg cgacgtgtcc ttctgcgaca cgtccttttt
      661 cgccgcgtcg gcgccttcct gcacacagta gacgactgtg tcctggccca tggcggcggc
      721 gagtgaggcg tacatgaacg cggcgcgcac ctggccgggc gcgttcaggc cagagcgcaa
      781 gacgaccaga aacaggtttt cgtccttcgc ggcgagttga ggcgaggcgg cctggcgtgc
      841 gcccttgcgc acgaagaagc tgtactgccc gttcgccgtg tcggagatcg tgacaagctc
      901 ggaggcgatg cgctcggccc acttcgccag atcgggtttc gtgttggggt cgtcggccat
      961 cacttccagc acctgaccgg cgggcatttc cagcagcgcc tgctctgctt tcagaatcgg
     1021 gacaggatac atcaggcccc ggcagtcaag cgtccgatca gcccgaggag tggccatggc
     1081 aatctcctgg aaggtgggaa tagatgacta atatgcccat aatactctga attaggagca
     1141 aagtcaaaac aaccttgtcc tgtttggctg gtggtatatc accgccgaga cgcacagtgc
     1201 gccgagtttc ccgttggaat gcgctccagt gtataatgcc tggcagctca ttgcgcacaa
     1261 gggtcgtcca gtgtcatccg atagtgtgac tctctttcat cgggcgttcg acaagttgta
     1321 tccctcgatc cggttcgtct acgagcgcat ccagggccac gcgtggttcg atcggatcac
     1381 gccgcgcggg aaggtgacgg cggagttgtg gatgggcggc gcgccgacgt atcggcgtga
     1441 ctacgagttc atcctgcagc atgggatcac ggcggtcgtc aacatccgct ccgagcgcgc
     1501 cgacgacacg gacttctacg acgcgcacgg cattactcac gtgcgctacc gggtgccgga
     1561 catcaccgtg cccgacgaga aagtgatcag cgatggcgtg gactggatca agcagcaggt
     1621 ggacgacggg cgagcggtgc tgattcactg cgccaagggg cgcggccgct cggcgacgct
     1681 gctggccgcc tacctgatgc gcgaagaagg catgacgtac gacgaggcat ggcagttggt
     1741 gaaatcgaag cgccgactgg ccaagctgga gaccaggcat cgcgagcggt tggaagcgtg
     1801 gatcgccagg aacggcaggc aggcgtgacc ggcgtccacc acagtgagat aaggttgtga
     1861 cgcccgagca agcgctttaa gggacgaaca gcgatcagcc attttcttgc gactggatag
     1921 gatgaaacgg ttagcaacat tccggaccga agatcgctgg attatcggct cgatggcttt
     1981 gactctgggt ggtgtgatgc tgggcgccat cctggccgag ccgcgcgctt tcggcatcac
     2041 cgccgtggtt gttatttgct tgctactgat cgcctggtat aagacccgca gccccaagct
     2101 ggcctggttg ctggtgttcg gcttcattgc cgggttgctg gagttgtggg ccgactgggt
     2161 ccacgtaaca tatttgaaat cccttatata cacggactac ttcggcttca accttctcgc
     2221 ttcgccttcc tacatgccga tcggttggtg gctaacagtc gtccagtttg gatatcttgg
     2281 attgcggctg acggaccgct ggcccatctg gaaggcagtc ggactactga ctttacttgg
     2341 gatgaccctg cccccgtggt atgaggagtt tgctgccccg gctcgagcct ggcattactc
     2401 tcctcaaggg atcatgctct ccaacacgcc gctgtggatt atcttgactt atggtgggtg
     2461 catgtttgcg attgggtcag cagccgtgtt gttgtattcg ccccgcgcct gggggcgggc
     2521 ggtgctgggt ggtatcttca ccggcgcgag tctgatgttt tcagggttat tttggttctc
     2581 cgtgttctga agaagctgaa aggtcttgat ttctcgacga tttcccctta tactgtgttc
     2641 agctagtgag gtgacaaccg aatgcctcgg cacgtgctat tgaccgagga tctgccaagg
     2701 tgaggagcct tggtgggaag gttggagata tacacctgcc cacctgtttg gtgtggcagg
     2761 tcttttttgt ttcttggccg tacagtctcg gtcgcaatca gcccagaagg gagcattcac
     2821 atggacaggt catctctcca ccgacaactg aatatcctgc tcgtcgcgct tctattgctg
     2881 gctttggccc tcggtctcgc gccatccgtt gctggggcca cgtccggtga caacccggcc
     2941 ggcgagggct tcaccgtgcg ggttgtatcc agcgccccgg accaggtgac tggaggcgac
     3001 gcccgcctgc acatcgtcgt gccactcatc gtacccctcc gccgggttgg ggtgtgggtc
     3061 aacggtgtcg atcagcgtag ccatttcagc gccctgcccg gcacacgcat tctgaccggt
     3121 gtgatcgacg gcctggcgat aggtgaaaac aggctggtgg tcaaggccga cggcagaggc
     3181 gagaggcgcc cggcgccagt ggccatgacc ctcaccaact acccgatcac cggcccggtc
     3241 ttttccggac cacatcagca tcccttcgtc tgcacagtga tgaccgaggg tctgggccag
     3301 ccgatccccg accaccccag caccggcacc aaggtcttcg acgccagcgg cagcgtcatc
     3361 ggctacagcc gcgactgctc cgtgcccacc caggtggtct ttaagtaccg caccaccgat
     3421 aacacgtgga aggactacac gcccggcatg ccccgcccaa gcgatatggc ccagaccacg
     3481 acgctcgacg gcctgacggt ggacttcatc gtgcgctggg agcgcggcac gatcaaccgc
     3541 ttcatctaca gcattgccgt gcttgccccg ttcgacgacg acgcctggga gctcaaccgg
     3601 ggcgcctgga acaagcgcgt catctaccgc ttcgacggcg gtgtggccat cgggcacacc
     3661 caaggccgca tcagcacatc gtccatgctt tatgacgtcg gcctggcgcg cggttatgcc
     3721 atcctctact cgacaggcac gcgcaccagc acgcactaca acctgcagct cggcggcgag
     3781 acggccttga tggtcaagga gcgcttcgtt gagctgtacg acgtgccgct gtacacggtc
     3841 ggcgtaggcg gctcgggcgg cgccattcag cagtacgtct acgcccagaa ccaccccggc
     3901 ctgctcgacg ccgccatccc gcagtactcg tatcccgaca tggtcaccca ggccgtgcac
     3961 atcggcgact gcgagctgct cgagttctat atggatgtac tggatggagt caacccacgc
     4021 tggaagacct ggaccaaccg cagcctgctg gaaggcctgg ccgccagcga cacgctgccc
     4081 aacccttaca acctgggcaa gcccggcctc accgagtgca tcaacggctg gcgggggctc
     4141 tcgccgctgg ccctcaatcc gttgtacggg ttggcgccca atcagcagtt ttacgagccg
     4201 ccgtcggcca tcgccgccat cgaatggacg cacttcgccg acatcgtcaa catagtcggc
     4261 cgggacgccg atggctatgc tcgtcgctac tgggacaatg tcggcgtgca gtacggcctg
     4321 caggcggttg ccagcgggca gatcacgcct gacgagttcc tcaagctcaa cgcgatggtg
     4381 ggtggttgga aggaagaggc cgacatggtt caggaaggct cgcccttcta tccacccggc
     4441 gtgatcgacc cggctaactg ggatccgtgg agccagcgca accaggtgta cagtctcgat
     4501 ccgctcaata acccagcccc gcgcacagcg ggcagcctgg aggccatgca ggcagtgtac
     4561 gaagccggcc tggtcttcac gggcgacatc gacatcccgg tcatcgactg gcgccactac
     4621 ctcgaggacg agctggacat gcacaactcg caccagtcgt tcgcctcccg ccagcgcatg
     4681 ctgaacgccg acggcgacgc cagcaaccag gtgatctggt tcaccgatgt ggtggtgagc
     4741 agccagcgct tcgaccagac ccccgaagcg ctcgaagtga tggacgagtg gatacttaac
     4801 atcctggctc accccgatct gggcgttgcc ggcaacaagc cgccacgcgc cgccgatcgc
     4861 tgtttcgacg ataagggggt ggagatcgct tccggtcccg atgtatggga cggcatcctg
     4921 aacgacgacc cgcccggggc ctgcacgcag cgcttcaagc tctacagcac gtcgcgccgc
     4981 gtggccggtg ggccattcga gcagagcatc ttcaagtgcc agctcatccc ggtcggtgaa
     5041 gctgtcgccc gcggattcta cggcgtctgg atgcccgacg cggcccagac gatgctgctg
     5101 cagcgcatct tctcccacgg cgtgtgtgat tacagccggc cggatcaggg attaccgccg
     5161 ggacactagc tgcatctacg tcttaccgct gccacgcaga cctggcaggt cttctaatct
     5221 aatgcgcagc caacatcggc gcccaagacc tgccaggtct tccacttgac ctccccgcga
     5281 atgtgagtat ccttgaagag ccaggggcag gcgcttcgcc g
//
LOCUS       DBNHXB010000359        14076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3117251, whole genome shotgun sequence.
ACCESSION   DBNHXB010000359 DBNHXB010000000
VERSION     DBNHXB010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 14076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14076
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3117251"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(324..395)
                     /locus_tag="AC162G_14635"
     tRNA            complement(324..395)
                     /locus_tag="AC162G_14635"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(361..363),aa:Met,seq:cat)
     gene            complement(481..867)
                     /locus_tag="AC162G_14640"
     CDS             complement(481..867)
                     /locus_tag="AC162G_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077092.1"
                     /translation="MSSIWRQVFYFKSAWLIGLALAALPLLGAFNARLAVSRQLLEDE
                     ARLQRAIDYERSRAQLLDQYEQYAKTDVYVEAWARRARMAKPGEIAVVPVIPLDTEEP
                     GQSSDTGRLPRDHVFEWWAVFFADLP"
     gene            complement(931..1014)
                     /locus_tag="AC162G_14645"
     tRNA            complement(931..1014)
                     /locus_tag="AC162G_14645"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(978..980),aa:Leu,seq:cag)
     gene            complement(1190..2956)
                     /locus_tag="AC162G_14650"
     CDS             complement(1190..2956)
                     /locus_tag="AC162G_14650"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5077093.1"
                     /translation="MFRHVRWQATLIVAGILILAALMAYLALSFTVRYVPAPGGTYIE
                     GVVGQPQFINPLLCSFNEPDRDICALVFNGLVKFDERGAPQPDLASRWTISQDDENYG
                     LIYTFQLRDDVTWQDGQPFTADDVIFTIGLLQDPSFPGRPDIGALWRSVEAVKIDDRT
                     VQITLQEPFAPFLDYAAVGMLPVHILNGVSAGELLDHPFNQQPLGTGPFHVKELVSEP
                     GQPQRIVLETNPRYFGRQPYINEVEFKYYATAAEAFEAYRAGEIHGISPITAADLDLV
                     RETPSLNLFTAPISGLSLIFLNTNDPNSPFFQEEDVRKALLLALDRQAIVDGALGAQA
                     VVANGPFHQNSWAYDPDTPPIEQDISRARQLLARAGWIPSEDVTGSANGDDSDLLPEA
                     DRPVAPRIETRRSDALRLKSGVPLSFTLLVSSDQVGVARAVANQWRVIGVNAEVRPVQ
                     IGLASNFLQARQYQAALANVVFDSPDPDPYPFWHETKASAGQNYSQFKDRDVSEVLEA
                     ARRVVDPTRRAELYRRFAQMFNEKVPAIVLYYPVYSYGVSQRIRGVQLGPLLTPADRL
                     QTLADWFVIERRVIANSGQLTP"
     gene            complement(3057..3281)
                     /gene="secG"
                     /locus_tag="AC162G_14655"
     CDS             complement(3057..3281)
                     /gene="secG"
                     /locus_tag="AC162G_14655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR5077094.1"
                     /translation="METAVSIAQVILGLVLIVLIGLEVRGQSMGGFLSSGDSPVYRTR
                     RGFERTLFNLTIVVSILFFAVSVWNVILAG"
     gene            complement(3374..4462)
                     /gene="cax"
                     /locus_tag="AC162G_14660"
     CDS             complement(3374..4462)
                     /gene="cax"
                     /locus_tag="AC162G_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015369 - calcium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HIR5077095.1"
                     /translation="MTRLKDWLGLSAPLVLMLVFVPAAIVAQLVEADPVIRFAASALG
                     VIPLAGLIGEGTEALADKTGPRLGGLLNATLGNAAELIIALAALREGLLSLVLASITG
                     SILGNLLLVLGAALLLGGIKNGVQTFDRAHAGLDATLLILSVIALGIPSLFNFAIEPN
                     FTSVEALSIGASIAMLVMYGLAVLHSLTSRVSEEDPLAREAHPPSGWSVRRALVVLAI
                     AVAFIVVLSEILVASVEPVTDALGLSEFFIGIILVPIVGNVAEHLVAVQVAVKNKMDL
                     SLSIAVGSSLQVALFVAPLLVLLSLVVGNPLALEFNSFELAAVAAASIIAAFVSLDGE
                     SNWLEGAMLLVVYAILALAFFFLVPGTA"
     gene            complement(4480..5430)
                     /locus_tag="AC162G_14665"
     CDS             complement(4480..5430)
                     /locus_tag="AC162G_14665"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR5077096.1"
                     /translation="MRRGLIIYNPVSGLSSASEELIRVRAYLAARGWSIDIEQTGGPG
                     DAGRLASEAANAGLYAALIAGGDGTLNEAVNALVGTDTAVGVLPCGTGNMWARQLGMP
                     SSVWRLLDAARSMNEASLRRVDLGRVTVRAGTPEAASRYFLLWAGVGLDGRITRSIEP
                     RPPWFKRWGIVSYALAALRIGVGYVGAQAEIEIDGRSLSEHVVMVLVCNADLYAGIFH
                     LAPEARLDDGCLNVSVIRGWGIGAVAGYFARVLLHRHVRVPSLVTLPARRVRIVTRPT
                     SDVHVDAEPLGSSPVECSIAPRALRVLVPPNAPAALFEPI"
     gene            5662..7575
                     /gene="pknB"
                     /locus_tag="AC162G_14670"
     CDS             5662..7575
                     /gene="pknB"
                     /locus_tag="AC162G_14670"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HIR5077097.1"
                     /translation="MESTTLLNNRYRLLSQVAAGGMAVVYKAQDTLLNRVVAVKILRE
                     SFAADPTFQARFRREAEAAANFNHVNVVTVYDVGHDAGRHYIVMEYVDGQDLKQIIRA
                     EAPLPIHRAVEIAIQMCAALGAAHRAGLVHCDVKPQNVLITADGRVKVTDFGIARVLS
                     QASTAITDTVWGTPHYISPEQAAGESPTPASDVYAAGVIMYEMLTGRLPFEGETHTQL
                     ALAHMRDEPPPLTLLNPTVPAQVDQIVSKVMSKEPSARYRTADQLATVLIEYRNVAEQ
                     ATGYQPATRPPPIGPTVERTPIRETLPVAQAEGFDWLGWLLGGLAAVAVLGLIILWVF
                     VARAYSVPAPIVTPQPAGTPASGSTNLPAANGQVPVPRVVGLTRDQAVMRLDEAGLRA
                     VDGALQFSSDVPAGIVLQQMPAPGELAPRNSIVQIVVSSGPQVSAVPNVTGLMYNPDI
                     EAGLKQSGWDVRTEEQYSQKPYQEILAMTPPPFTLLPAGEPLTLTLSGGITLTMEAQF
                     AGLIQLDSVLLPAAEVRRGELLEMTFLWRAMERIGTPYVRFIHFVDADGEIQTQADAE
                     PSPPTTAWARNVLIPDQYFLPIPGGVLPGEHQLLVGFYPVGSAFTRLPISDPGKSQDV
                     DQRLLVYTVTVLP"
     gene            7751..9703
                     /gene="ftsH"
                     /locus_tag="AC162G_14675"
     CDS             7751..9703
                     /gene="ftsH"
                     /locus_tag="AC162G_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR5077098.1"
                     /translation="MNTGRSRNNFVYLIIIIAIGAILVSVLRGQAPSNEDISLTELAS
                     MINAGKVASITQSDLNLTVTLDNDDVVTTRAAAGSPATDQLIALGADPEMFHEANPKA
                     VEFSVESPPEWGTWLSLLSYMIPALFVVGLIYFMFRQAQGSNNQALSFGKSRARMITS
                     EHPTITFDDVAGAEEAKQELAEVVEFLKEPEKFISLGARIPKGVLLVGSPGTGKTLLA
                     KAVSGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRHSPCIVFVDEIDAVGRHR
                     GAGLGGSHDEREQTLNQILVEMDGFDTDTNVIVVAATNRPDILDPALMRPGRFDRRVV
                     LDRPDFNGRQGIFKVHVRGKPLAEDVDIEKLAKATPGFVGADIENTVNEAAILAARRN
                     RKAIMMTDMEEAIERVLLGPERKSRIILPKEKKITAYHEAGHSVAMRALPNSDPVRKV
                     TIIPRGIAAGVTWSMPEEDISHMTRSKLKDLMAGILGGRAAEELVFSGEITTGASNDL
                     EKVTEIARSMVTRWGMSDKLGPLVYGKKEELVFLGKEIGEQRDYSEAVAQEIDSEVRK
                     LVAEAHDTALRVLTEHRDQLDAVAQALMEYETLDMAEFEAVWNGQPPRPSKGAAPSAP
                     APTTGPGKPAPEPPTPGGKQPVPVPA"
     gene            complement(9874..11601)
                     /locus_tag="AC162G_14680"
     CDS             complement(9874..11601)
                     /locus_tag="AC162G_14680"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004467 - long-chain fatty acid-CoA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HIR5077099.1"
                     /translation="MHKPWVKFYDEGVPESLKPYPEHPLYVFLEQTAAKYPNKPAIHF
                     KPSHQGFAKSSLTYAQLNDLSDRLAAALAALGVKKGDRVAIFMPNIPQFVIAFYGVLK
                     AGGVVVATNPAYTEPEIEYQLKDSGAETMICMSLFYPKVQKVLANTRLKNVIVTNIKD
                     YMSGLLRLLFTLAREKKDGHRVELSPGHLSFMDLMAKYSASQRPKVDVGPDDRAVFQY
                     SGGTTGVPKAAIALHRHLVANTLQIRAWFSKCREGEETVLLAIPLFHVYGLVAGMSFA
                     VQGGASMVLVPNPRDVHSIVDAIDAYQPTVFPGVPRMYNGLNNFPGIKTHKVGSIRWC
                     ISGSEPLLLDVKSKFESITGGKLVEGFGMSECPTAAIVNPLIGLNKPGSIGVPYPDTE
                     ARIISLEDEVTPLKAGEVGELCLRGPMVMYGYHNMPTETQNTLRRHPDDPDGPPWLHT
                     GDTATMDEDGYVFIVGRKKELIKVGGLQVWPREIEDDLIKHPAVLEAAVAGVPDQQRG
                     GESVKAWVVLKPGASATEDELKKYLSERLVSYKVPRQIEFRSELPKSAVLKVLRRTLV
                     EQETMKATA"
     gene            complement(11791..12201)
                     /locus_tag="AC162G_14685"
     CDS             complement(11791..12201)
                     /locus_tag="AC162G_14685"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR5077100.1"
                     /translation="MTANEVFETTFPVRYAETDQMGIAHHASYVVWMEEGRSQYMRAK
                     GIAYEEIEREGRFLAVADLNVRYVAPARYGDAVTVRTWIGELRSRTVTFGYEVLHATS
                     RNLLATGKVKLVWIDRRGRVTKMPDRVRSVIGGG"
     gene            12498..13115
                     /gene="lexA"
                     /locus_tag="AC162G_14690"
     CDS             12498..13115
                     /gene="lexA"
                     /locus_tag="AC162G_14690"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR5077101.1"
                     /translation="MKTLTHRQLAVLSFIETFIASNRYPPTYEEIRRGLGLSTKSLVN
                     HHLNALEEAGVIERDRLTSRGLRLAGQSVHRVPVVGSIVAGLPVTSFAESIDTAREAL
                     LLTSDIVAPQEGLFALRVRGDSMIDAMVSDGDLVVMKRQSDVRDGDMVAVWLKDRDET
                     TLKHFYRENGQIRLQPANPTIGPIFAHPRNVEVQGKVVAVIRRVN"
     gene            13221..>14076
                     /locus_tag="AC162G_14695"
     CDS             13221..>14076
                     /locus_tag="AC162G_14695"
                     /inference="COORDINATES: protein motif:HMM:NF013727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR5077102.1"
                     /translation="MRQLLELQADRIEMVLAHHKAPARVAGGVVTPRWVQFHLAPALG
                     TRLHRVQALADELALALGAPQVRVSRHGAALHLEVPRPHPEPVRLLDLCRRLPAVPLG
                     TAVLGIADDGAPLLLRLPSPDVAHVLVAGTTGSGKTALAQAIVLSLALAHRRSQLQLV
                     LVDPKAGRAFAALAGLPHLLRPVVVDAQAAATTLVELTAVMEQRDRQRVSTPRIVVVI
                     DELADLVATAGKPALEALTRLAQRGREAGLHVVACTQKPSAALLGAVTKANFPVRLVG
                     RVTSIEDAR"
BASE COUNT         2702 a         4655 c         4328 g         2391 t
ORIGIN      
        1 tgtgctgacc cgcacgtatc cctggagcgt atatgcggag caggccagat agctgcgcag
       61 gtcttgcgtc gtcaggctgc tgatgtcaac gtcaccggct ggggacagcc acgtgcggag
      121 gtcgcgctta gaactttgag cgtgcaagac caagggcttc tgccgcttta ccgaaggtgt
      181 gggaagccgg ggatggcctt agaaagcttg aggcccggcg gtcagctgtt catcgaaaaa
      241 ccctccaggt cgggggtctt ccgaaccggc catcaccggg ccaacaaaaa caccgtagcc
      301 tggcggacta cggtgtcatc cagtagcggg gctaggattc gaacctagga cctccgggtt
      361 atgagcccga cgagctgcca ctgctctacc ccgcaacgcg cccacatggg ctacactgac
      421 aaagaagcgg gacacacctc catcccgcaa tttgcattat accatgtgtg ccacaagccg
      481 tcaaggcagg tctgcaaaaa acacagccca ccactcgaac acgtgatcgc gcggaagtct
      541 gccggtatca gacgactggc ccggctcctc ggtatccaga ggaatcaccg gcacgacggc
      601 gatctcacct ggcttcgcca tgcgcgcgcg gcgcgcccat gcctccacgt agacatccgt
      661 cttggcatac tgctcgtact ggtccagcaa ctgcgcccgg gaccgctcgt aatcgatcgc
      721 gcgctgcaac cgcgcttcgt cctcaagcaa ttggcgactc acggccagac gcgcattgaa
      781 cgcgcccaac aacggcagag cggcaagcgc caacccgatc agccacgcag atttgaagta
      841 gaaaacctga cgccaaatgc tcgacaccga ggctccgacc cgcagccgcg aatataaaat
      901 cccaggcgcg cctgggattg gcagtcgagg tgccgaagct gggacttgaa cccagatgag
      961 ggaaccctca ctgcgccctg aacgcagcgc gtctgccaat tccgccactt cggcatgcta
     1021 cagcgatgag tattctactc gaatcgatct gtttgtcaag ccacgctctc tggtgattgc
     1081 acatctgttg gagtgacagt tgcacacaac cagcgtctgc agttgcactg cggacgaacg
     1141 gaattgtgga taatcaccag acactctcca actgacgcgt gcccgtcctt cacggcgtga
     1201 gctggccgct gttggcaatg actcgccgct cgatcacgaa ccagtcggcg agcgtctgca
     1261 ggcgatcggc aggcgtcagg agcgggccga gctgcacgcc gcgaatgcgc tggctgaccc
     1321 cataactgta cacgggataa tacaacacga tcgcgggcac cttctcgttg aacatctggg
     1381 cgaaccggcg gtacaactcc gcccgccgcg tcggatcgac cacccgccgc gcagcctcca
     1441 agacctcgct gacgtcccga tctttgaact ggctgtagtt ttggcctgcc gacgccttcg
     1501 tctcgtgcca gaagggatag ggatccggat ccggcgagtc gaacaccacg ttcgccagcg
     1561 ccgcctggta ctgccgcgcc tgcaggaagt tgctcgccaa cccgatctgc actgggcgaa
     1621 cctcggcgtt gacgccgatc acgcgccact ggttggcgac ggcccgcgcc acgccgactt
     1681 gatcgctcga caccagcaac gtgaaggaca gcggcacgcc gcttttcagg cgcagcgcgt
     1741 ccgaccgtcg cgtttcgatg cgcggggcta ccggcctgtc ggcttcgggg agcaggtcgc
     1801 tgtcgtcgcc attcgcgctg cctgtgacat cctccgacgg gatccacccc gctcgcgcga
     1861 gcaactgccg cgcccgggag atgtcctgct cgatgggagg cgtatccgga tcgtacgccc
     1921 aagaattttg gtgaaagggg ccgttcgcca cgacggcctg cgctcccaac gcgccgtcga
     1981 cgatagcttg ccgatcgagc gcaagcagca gcgccttccg gacgtcctct tcctgaaaga
     2041 acggcgagtt cggatcgttc gtattgagga agatgaggct gagacctgag atgggcgccg
     2101 tgaacaggtt caacgaaggc gtctcgcgca ccagatcgag atcggctgcc gtgatgggcg
     2161 atataccgtg gatctcgccg gcgcgatacg cctcgaaggc ctccgccgcc gtcgcgtaat
     2221 acttgaactc cacttcgtta atataaggct ggcgaccgaa gtagcgagga ttggtttcca
     2281 gaacaatgcg ctgcggctgg ccaggctcgc tcaccagctc cttcacgtgg aatgggccag
     2341 tgcctaatgg ctgctgattg aaaggatgat cgagcagctc gccggcgctc acaccgttga
     2401 ggatgtgcac cggcagcatg cccacggccg cgtagtcgag gaagggcgcg aaaggctcct
     2461 gcagggtaat ctggacggtg cggtcgtcaa tcttgaccgc ctcgaccgaa cgccacagcg
     2521 cgccgatgtc cgggcgtccg gggaagctcg ggtcttgcag caggccgatc gtgaaaatga
     2581 cgtcgtcggc agtgaacggc tggccgtcct gccacgtcac gtcgtcacgc agttgaaacg
     2641 tatagatcag gccgtagttc tcgtcgtctt gagagatcgt ccatctcgag gccagatccg
     2701 gctgaggcgc tccgcgctcg tcgaacttga cgagaccgtt gaagaccagg gcgcagatgt
     2761 cgcgatcagg ctcgttgaaa gagcacagca gcgggttgat gaactgcggc tggccgacga
     2821 cgccctcgat atacgtgccg cccggcgcag gcacgtaacg gacggtgaac gagagggcca
     2881 gataggccat cagcgcggcc agaatcagaa tacccgcgac gatgagcgtc gcttgccagc
     2941 gcacgtgccg aaacacgcca aaccactcct tctcaagaaa acgagggtgt caggcagcgg
     3001 ccgcctcaac acccccttat gatgggtacc gcgattgttg tcgatttcgc aggccgtcag
     3061 cccgccagga tcacattcca gacggaaacg gcaaagaaca ggatcgacac gacgatcgtc
     3121 aagttgaaca aagtccgctc gaaaccccgg cgcgtgcggt acacgggact atcgccgctg
     3181 ctgagaaagc cgcccatgga ttgcccacgc acctcaagtc cgatcaatac aatcagcaca
     3241 agacccagaa tcacctgagc gatacttacg gcggtctcca cctacctctt ctcctcatgt
     3301 cagactgaat aacattatag cacgcccctc aggcccgaac aagcaccggg agcagggcca
     3361 gtacattctc ctgctaggcc gttccgggca caaggaaaaa gaacgccagc gcgagaatcg
     3421 cgtacacgac cagcagcatc gccccttcca gccagttgct ctcgccgtcc agcgacacga
     3481 acgccgcgat gatcgaggct gccgccaccg cggccagctc gaagctattg aactccagcg
     3541 cgagcgggtt gcctacaacc aggctcagca gcaccaggag cggagcgacg aacagcgcga
     3601 cttgcaggct cgatccgacg gcgatgctca gacttagatc cattttgttc ttaacggcca
     3661 cctggacggc aaccagatgc tccgcgacgt tgcccacaat cggcaccagg atgatgccga
     3721 tgaagaattc gctcaggcca agcgcatccg taacaggctc gaccgaagcg accaggattt
     3781 cgctcaagac gacgatgaag gccacggcga tggccagcac gacgagcgcc cggcgcacgc
     3841 tccaacccga cggcggatgc gcctcgcgcg ccagagggtc ttcctcggag acgcgcgaag
     3901 tcagcgagtg caggacggcc aggccataca tgaccagcat ggcgatcgac gcgccgatgc
     3961 tcaaggcctc gaccgacgtg aagttcggct cgatcgcgaa gttgaacagc gacggaattc
     4021 ccagcgcaat gaccgacagg atcagcaacg ttgcgtcgag gccggcgtgc gcgcgatcga
     4081 acgtctgaac cccgttcttg attccgccca gcagcagcgc ggcacccagc accagaagca
     4141 ggttgcccag aatcgacccg gtgatcgagg ccagcaccag gctcagcaag ccctcgcgga
     4201 gcgcagccag cgcgatgatc agctccgccg cattgccgag cgtcgcattc agcaggccac
     4261 ccagccgagg cccggtcttg tccgccagcg cctcggtccc ttcgccgatg agtcccgcca
     4321 gtgggatcac gcccagcgcc gacgccgcaa aacggatcac ggggtcagct tcgacgagct
     4381 gtgcgacgat cgcagccggg acgaagacaa gcatgaggac gagcggcgcg ctcaagccca
     4441 gccagtcttt caatctcgtc atcaccggca tcccctctct caaatgggtt cgaacaacgc
     4501 ggcaggcgca ttcggcggca cgagcacgcg cagcgcgcgc ggcgcgatcg aacactcgac
     4561 cggcgaggag ccgagcggct cggcgtcgac gtgcacgtcg ctcgtcgggc gcgtcacgat
     4621 gcgcacgcgt cgggccggca gcgtcaccag gctgggaacg cgcacgtgtc ggtgcagcag
     4681 cacgcgagcg aagtaaccgg caactgcacc gatgccccag ccacgaatca cactcacatt
     4741 caggcagccg tcatccaggc gcgcttccgg cgcgagatgg aatatgcccg cgtacaggtc
     4801 ggcgttgcac acgaggacca tgacgacgtg ctccgacagg ctgcggccat cgatttcgat
     4861 ctcggcctgc gcgccgacgt agccgactcc aatgcgcagc gccgccagcg cgtagctgac
     4921 gattccccag cgcttgaacc acggcgggcg cggctcgatg ctgcgggtaa tacgcccatc
     4981 cagccctacg cccgcccaca gcagaaagta gcggctggcc gcctccggcg tgccggcgcg
     5041 cacagtgacc cggcccaaat cgaccctccg cagcgacgct tcgttcatcg agcgcgcggc
     5101 atccagcagt cgccacactg aggatggcat gcccagttgc cgggcccaca tgttacccgt
     5161 gccgcagggc aacacaccga cagccgtgtc cgtcccgacc aacgcgttga cggcttcgtt
     5221 gagcgtgccg tcgcctccgg cgatcaaggc ggcatacagg ccggcgttgg ctgcttcact
     5281 cgccagccgg ccggcatcgc ccggtccgcc tgtctgttct atgtcgatcg accagccgcg
     5341 cgcggcgaga taagcgcgca cccggatcag ctcttcggaa gcgctgctca gcccggacac
     5401 aggattataa atgatcagcc cgcgacgcat tagccgcgat tataacaagg acgatgcttg
     5461 ctcgcaacac acggcgcgct gcggtgcgca cgtagaggtt gtcaggcagc catcccttcg
     5521 actccgcggc gcgcgtcctt cgattccact gcgttccgct cagggtgcgg ctgacctgtt
     5581 tgcacacgcg ccgccgggca ccctctgtgt ggcaagcccc atgactttga ggtaaaatac
     5641 cgcgcgagcg tcgggcacgt catggaatcg accacactcc tcaacaaccg gtaccgcctg
     5701 ctgtcgcaag tcgcggcggg cggcatggcc gtcgtgtaca aggcacagga cacgctgctc
     5761 aatcgcgtcg tcgcggtcaa aatcctgcgc gagtccttcg ccgccgaccc cacgttccag
     5821 gcccgcttcc ggcgcgaagc cgaggctgcg gccaacttca accacgtcaa cgtcgtcacg
     5881 gtgtacgacg tcggccacga cgccggacgg cactacattg tcatggaata tgtcgacggg
     5941 caggatctca agcagatcat tcgcgccgaa gcgccgctgc cgatccaccg cgccgtcgaa
     6001 atcgcgattc aaatgtgcgc cgcgctgggc gcagcccatc gcgccggact ggtgcactgc
     6061 gacgtcaagc cgcagaacgt gctgatcacc gccgacggcc gggtcaaggt caccgacttc
     6121 ggcatcgcgc gcgtgctctc gcaagcatcc acggcgatca ccgacacggt gtgggggaca
     6181 ccacattaca tctcgcccga gcaggccgcc ggtgagtcgc cgacgccggc cagcgacgtg
     6241 tacgccgccg gcgtgatcat gtacgaaatg ctcaccggcc gcctgccgtt tgagggcgaa
     6301 acgcacaccc agctcgcgct cgcccatatg cgtgacgagc cgccgccact cacgttactc
     6361 aacccgaccg ttcccgcgca agtcgaccag atcgtcagca aggtgatgag caaagagccg
     6421 tcggcgcgat accgcaccgc cgatcagctc gccaccgtcc tcatcgaata ccgcaacgtg
     6481 gccgagcagg ctacaggcta ccagcctgcc acgcgccccc cgccgattgg tccgaccgta
     6541 gagcgcacgc ccatccggga aacgctgccg gtcgcccagg cagagggctt cgactggctc
     6601 ggatggctgc tcggcggcct tgcagccgtc gctgtgttgg gcctgattat cttatgggtc
     6661 ttcgtcgccc gcgcctattc tgttccggcg ccgatcgtca caccgcagcc cgctgggacc
     6721 cccgcttcag gctcaaccaa cctgccggca gcgaacggtc aggtccccgt tccgcgcgta
     6781 gtcggcctga cacgcgatca ggcggtgatg cggctcgacg aggccggcct gcgcgcggtc
     6841 gatggcgcgc tgcagttcag cagcgatgtc cctgccggaa tcgtcctgca gcagatgccg
     6901 gcccccggcg aattggcccc gcgcaattcg atcgtccaga tcgtcgtcag cagcggcccg
     6961 caagtctcgg cggtgcccaa cgtcaccggt ctcatgtata acccggacat cgaggcaggc
     7021 ctcaagcaga gtggctggga cgtgcggacg gaagagcagt acagccagaa accctatcag
     7081 gagattctgg cgatgacccc cccaccgttc acactgctgc ccgccggtga accgctcacc
     7141 ctgacgctca gcggcggcat caccctgacg atggaggcac agtttgccgg cctgatccag
     7201 ctcgacagtg tgctgctccc ggcggccgaa gtgcgccgtg gcgagctgct tgagatgaca
     7261 ttcttgtggc gcgccatgga gcggatcggc acaccttatg ttcgcttcat ccacttcgtc
     7321 gacgccgacg gagaaatcca gactcaggct gacgccgagc cttcgccgcc gactacggcc
     7381 tgggctagaa acgtgctgat ccccgaccag tacttcctgc ccattcccgg cggcgttctc
     7441 ccaggggagc accagcttct cgtcggcttt tacccggtcg gatcggcctt tacccgcctg
     7501 cccatcagcg accccggtaa gtcccaggat gtcgatcagc gactcctggt atatactgta
     7561 acggtgctgc cctgatacat ggtggacagc ccgccaacaa gttgacctta cgcttttcga
     7621 cgcaaattcc gggtccgcgt ccccagcccg gcgcccgggc gcgggatctc taatgaaaca
     7681 tttacgggat agtcacggcg tttttgtaag aagtggggta taatgttggc gggatagatg
     7741 gaggtatgct gtgaacacag gccgatcgcg aaacaacttc gtgtacttga tcattatcat
     7801 cgccatcggg gcgattctgg tgtctgtctt gcgcggccaa gcgccgagca acgaggacat
     7861 ttcgttgacc gaactggcaa gcatgatcaa tgcgggcaag gtcgcgtcga ttacccagtc
     7921 cgatcttaat ttaacggtca ctctggacaa tgacgatgtg gttacgacgc gggctgccgc
     7981 aggctccccg gcgactgatc agttgatcgc actgggcgcc gatcccgaga tgttccacga
     8041 agccaatcct aaagccgtcg agttcagcgt cgaaagcccg cccgaatggg gcacctggct
     8101 cagcctgctc tcgtatatga tcccggccct gttcgtggtg ggcttgatct acttcatgtt
     8161 ccggcaggcg caaggctcca acaaccaggc gctttcgttc ggcaagagcc gcgctcgcat
     8221 gatcaccagc gagcacccga cgattacctt cgacgacgta gccggcgcgg aagaggccaa
     8281 gcaggagctg gccgaggtcg tcgaattcct gaaagagcct gagaagttca tctcgctcgg
     8341 ggcgcgcatt ccgaagggcg ttctgctcgt cgggtcgccc ggcacgggta agacgctttt
     8401 ggccaaggcc gtgtccggcg aggcgggcgt gcccttcttc agcatcagcg gctccgagtt
     8461 cgtcgagatg ttcgtcggcg tcggcgcgtc gcgcgtgcgc gacctgttcg atcaggccaa
     8521 gcgccacagc ccgtgcatcg tgttcgtcga cgagatcgac gccgtcggcc gccatcgcgg
     8581 cgcgggcctg ggcggctcgc acgacgagcg cgagcagacc ttgaatcaga tcctggtcga
     8641 gatggacggc ttcgacactg acacgaatgt gatcgtcgtc gccgcgacca accggcccga
     8701 cattctcgac ccggcgctga tgcgcccggg ccgcttcgac cgccgcgtcg tcctggaccg
     8761 ccccgacttc aacggacgcc agggcatctt caaggtgcac gtgcgcggca agccattggc
     8821 cgaagacgtc gacatcgaga agctggcgaa ggcaacgcct ggcttcgtcg gcgccgacat
     8881 cgagaacacg gtgaacgaag ccgccatcct ggccgcgcgg cgcaatcgca aagccatcat
     8941 gatgacggat atggaagagg cgatcgagcg cgtgctcctc ggcccggagc gcaagagccg
     9001 catcatcctg ccgaaagaga agaagatcac ggcctaccac gaggccggtc actccgtcgc
     9061 gatgcgcgcg ctgcccaaca gcgacccggt gcgcaaggtc accattatcc cgcgcggcat
     9121 cgccgccggc gtcacctgga gcatgcccga agaggacatc tcgcatatga cgcgctcgaa
     9181 gctcaaggac ctgatggccg gcatcctggg cggacgcgcc gccgaagagc tggtgttctc
     9241 cggcgagatc accaccgggg ccagcaacga tctcgaaaag gtcacggaga tcgcccgctc
     9301 gatggtcacc cgctggggca tgagcgacaa gctaggccca ctggtgtacg gcaaaaagga
     9361 agagctggtc ttcctcggca aggagatcgg cgagcagcgg gactacagcg aagcggtggc
     9421 ccaggaaatc gacagcgaag tccgcaagct ggtcgccgag gcgcacgaca ccgcgctgcg
     9481 ggtcctgacc gagcatcgcg accagctcga cgccgtggcg caggcgctca tggaatatga
     9541 gacactcgac atggccgagt tcgaagcggt gtggaacggc cagccgccgc gcccgagcaa
     9601 gggcgctgcg ccatccgcgc cggcgccgac gaccgggccg ggcaagcccg cccccgaacc
     9661 gcccacgccc ggcggcaagc agccggtgcc ggttccggcg taagccacga atcaatgaac
     9721 gaaacccgac ctccatgcga ggccgggttt tttcatcgca tatgcagaca acggcaggcc
     9781 ggcacactac tattctgccg ccagccgaac ttccttggcc gtggtgcgct tcacgcttcg
     9841 agcgcgtgac catgaatcgg ccaggcacac cggtcaggcc gtcgctttca tggtctcttg
     9901 ctcgaccagg gtgcggcgca gcaccttcag cacggccgac ttgggcagct cgctccggaa
     9961 ctcgatctgt cgaggcactt tgtaggaaac cagcctctcg gacaggtatt tcttaagctc
    10021 gtcttccgtg gcgctcgcgc cgggcttgag cacgacccac gccttcaccg attcgccgcc
    10081 gcgctgctga tctggcacgc cggccaccgc cgcttccagc acggcgggat gcttgatcag
    10141 atcatcctct atctcgcgcg gccacacctg gagcccgccg accttgatca attccttctt
    10201 gcggcccacg atgaagacgt aaccgtcctc gtccatcgtg gccgtatcgc cggtatgcag
    10261 ccagggtggg ccatcgggat cgtcagggtg ccggcgcagt gtgttctgcg tctcggtcgg
    10321 catgttgtgg taaccgtaca tcaccatagg gccgcgcagg cacagctcac ccacctcgcc
    10381 cgccttcaac ggcgtgactt cgtcttccag gctgatgatg cgggcctccg tgtcggggta
    10441 aggcacgcca atcgatcccg gcttgttgag gccgatgagc ggattgacga tggcggccgt
    10501 cgggcactcc gacatgccga agccctccac cagcttgccg cccgtgatcg actcgaactt
    10561 gctcttcacg tcgagcagca gcggctccga gccggaaatg caccaccgga tggaaccgac
    10621 cttgtgcgtc ttgataccgg gaaagttgtt cagcccgttg tacatgcgcg gcacgccggg
    10681 aaagaccgtc ggctggtacg cgtcgatggc gtcgacaatg gagtgcacgt cgcgcgggtt
    10741 cggcacgagc accatcgacg cgccgccctg gacggcgaag gacatacccg ccaccagtcc
    10801 gtagacgtgg aagagcggga tcgccagcag caccgtctcc tcgccctcgc ggcacttcga
    10861 gaaccaggca cggatctgca gcgtgttggc caccaggtgg cgatgcaggg cgatggccgc
    10921 cttcggcacg cccgtcgtcc cgcccgaata ctggaacacc gcccggtcgt ccggcccgac
    10981 gtcgaccttt gggcgctgcg acgcgctgta cttcgccatc aggtccatga acgacagatg
    11041 gccgggcgac agctcgacgc ggtggccgtc tttcttctct ctagccagcg tgaacaacag
    11101 ccgcaaaagg ccgctcatgt agtccttaat gttcgtcacg atgacgttct tgagccgggt
    11161 gttcgccaga accttctgta ctttcggata aaagaggctc atgcagatca tcgtctcggc
    11221 gccgctgtcc ttcaactggt actcgatctc aggctcggtg taggccgggt tcgtggcgac
    11281 gacgacgccg ccggctttga ggacgccata gaaggcgatg acgaactgag gaatgttggg
    11341 catgaagatg gcgacgcgat cgcctttttt cacgcccagc gcggcgagcg cagcggccaa
    11401 ccgatcggat aggtcgttga gctgcgcgta agtcagtgag cttttggcga atccctgatg
    11461 ggatggcttg aagtgaatgg ccggcttgtt gggatacttg gcggcagtct gttcgagaaa
    11521 gacgtagagc ggatgctcag gatacggctt gagggactcc ggcacgccct cgtcgtaaaa
    11581 cttaacccac ggcttgtgca tgacaggtcc tcctcgagga gttggttgca gcagtcggcg
    11641 ctacccggca gtatagccta cactcgcatc gaaagtcaac tgactaacgt aactcttccg
    11701 ctagtgccac ccggtcagcg ccagcgcggc agccgtggaa taaacacgct cgtcgtcagc
    11761 atatattccc ggtagcccgg ctgaaatctc ctatccgccg ccgatgaccg accgcacccg
    11821 gtcgggcatc ttggtgacgc gcccgcgccg gtcgatccag accagcttca ccttgccggt
    11881 cgcaagcaga ttgcgcgatg tcgcgtgcaa gacttcgtat ccgaacgtca cggtgcggct
    11941 gcgcagctcg ccgatccacg tgcgcaccgt gacggcgtcg ccgtagcgcg cgggcgcgac
    12001 gtaacgcacg ttgagatcgg cgacggccag aaacctgccc tcgcgctcga tctcttcgta
    12061 ggcgatccct ttcgcccgca tgtattgcga gcgcccttcc tccatccata ccacgtaact
    12121 ggcgtgatgg gcaatgccca tctgatcggt ttcggcgtag cgcaccggaa aggtggtctc
    12181 gaaaacctcg ttcgcagtca acgcccttcc ctccggcaga gtgcgcacat tttacaactg
    12241 atttcgacaa agtcgcaacc gctttgaagt ttggagtttg aggttgggag ttggagccag
    12301 gcgagagatt cctcactacg ttcggaatga cacgccgctg gacgtgatct gcctacaaat
    12361 cggcatctac ctgagggaga aacgccatat atggggcttg acagaatagg acaactgttc
    12421 tatactttca gtacagatag aacaaccgtt ctattttgag cgctcgtcct ttctccagca
    12481 atatccaggg ggttaccatg aaaacactca cccatcgcca gttggcggtt ctcagcttca
    12541 tcgagacttt catcgccagc aatcgctatc cgccgaccta cgaggagatc cggcgcgggc
    12601 ttgggctgtc gaccaagtcg ctcgtcaacc accacttgaa tgcgctggag gaagccggcg
    12661 ttatcgagcg ggatcgattg acgtctcgcg ggctgcggct cgccgggcag agcgttcaca
    12721 gggtcccggt cgtcggctcg atcgtcgccg gcctgccggt gacgtcgttc gccgaatcga
    12781 tcgacacggc ccgtgaagcg ctgctgctca ccagcgacat cgtcgcgccg caggagggct
    12841 tgttcgcgct gcgcgtgcgc ggcgactcga tgatcgacgc gatggtcagc gacggcgatc
    12901 tggtagtaat gaagcggcag tccgacgtgc gcgacggcga catggtcgcg gtctggctga
    12961 aagaccggga cgagacgacg ctcaaacatt tctacaggga gaacgggcaa atccggcttc
    13021 agcctgccaa cccgacgata gggccgattt tcgcccatcc gcgcaacgtc gaagttcagg
    13081 ggaaagtcgt cgcggtcatc cgacgcgtaa actgatagcc tatccggggc aattcggaat
    13141 gtggacttta gtctgcttca ggctaagcag gctaaagcct gcactccggt tgtcggatga
    13201 gtactagaaa gaggtgactt atgcgccagc tgctggagct gcaagcggac cgaatcgaga
    13261 tggtgctggc tcaccacaaa gcccccgccc gcgtggccgg cggcgtcgtc accccgcgct
    13321 gggtgcagtt ccacctggcc cccgccctgg gcacgcgcct gcaccgcgtc caggccctgg
    13381 ccgacgaact ggccctggcg ctgggcgctc cccaggtgcg cgtctcgcgc cacggcgcgg
    13441 cgctgcacct ggaagtcccc cgcccccacc ccgagccggt gcgcctgctg gacctgtgcc
    13501 gccgcctgcc ggccgtgccg ctgggcaccg ccgtgctggg catcgccgac gacggcgcgc
    13561 ccttgctgct gcgcctgccc tcgccggatg tggcgcatgt cttggtggca ggcaccaccg
    13621 gctcgggcaa gaccgccctg gcgcaagcca tcgtcctcag cctggcgctg gcgcaccgcc
    13681 gcagccagtt gcagctcgtg ctcgtcgacc ccaaggccgg gcgcgccttc gccgcgctgg
    13741 ccggcctgcc gcatctgctc aggccggtcg tcgtggacgc ccaggccgcg gcgacgacgc
    13801 tggtggagct gaccgctgtg atggagcagc gcgaccggca gcgcgtgagc acgccgcgca
    13861 tcgtggtggt catcgatgag ctggccgacc tggtggcgac ggcgggcaag ccggcgctgg
    13921 aggcgctgac gcggctggcg cagcgcggac gcgaggcggg gctgcatgtg gtggcctgca
    13981 cgcagaagcc gtcggcggcg ctgttgggcg cggtgacgaa ggcgaacttc ccggtgcggc
    14041 tggtggggcg cgtgacgagc atcgaggatg cgcggg
//
LOCUS       DBNHXB010000360        24463 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2448143, whole genome shotgun sequence.
ACCESSION   DBNHXB010000360 DBNHXB010000000
VERSION     DBNHXB010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 24463)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24463)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24463
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2448143"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..584)
                     /locus_tag="AC162G_14700"
     CDS             complement(141..584)
                     /locus_tag="AC162G_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077103.1"
                     /translation="MPRRPRHTSARCAEHDCPMIQIDDETVCALEHIDAHLGGKQVKD
                     LVVVEDGLITVLFEDDHELPLLCPHCGDPIGTDENELLDELAGLYLVGLEYVPADPEA
                     DVYEGVEFLFASDPEVDVNDMPDDVATSSILLHLDSVREIVCPGQ"
     gene            complement(658..1389)
                     /locus_tag="AC162G_14705"
     CDS             complement(658..1389)
                     /locus_tag="AC162G_14705"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR5077104.1"
                     /translation="MIPLRDANPTRHTPIVTVALIVANVAVFLFQESLPERSYTGFIF
                     SYAVTPALITADFGAYAHTLVTSMFLHGGWLHLLSNMLYLWIFGNNIEDRLGSVRFPL
                     FYLVCGLAASAAQIAINPSSEIPMIGASGAISGVLGAYILLFPNARVLTLVFLFFLVR
                     FVELPAGWLLGFWFLMQLLSGIGTLGLVRTGGVAFFAHIGGFVAGFLLVRLFAPSRRG
                     RPLPRDDLWIPTIDDDDRPPRGDRW"
     gene            1435..1764
                     /locus_tag="AC162G_14710"
     CDS             1435..1764
                     /locus_tag="AC162G_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HIR5077105.1"
                     /translation="MSESPEIAPAMPDIGVEFTYDEAAELDPPHDVIVHNDDVTPFDF
                     VVAVIHSVFELPPHDAYEVTLTAHYEGEAYVATLPIEDAKYRVYRAHSAARARGYPLT
                     FSIHPAH"
     gene            complement(1758..3125)
                     /gene="folP"
                     /locus_tag="AC162G_14715"
     CDS             complement(1758..3125)
                     /gene="folP"
                     /locus_tag="AC162G_14715"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR5077106.1"
                     /translation="MSARAYLGLGSNLDDRHGNLIDAIDRLRARAGLVDLSSVYETEP
                     WGVADQPRFLNSVCAIQTDLSPRDLLVWLKAIEADMGRRPAVRYGPRLIDIDLLLYGD
                     EVVEAPAVTVPHPRMAERAFVLAPLCEIAPDAVHPVLGESVRELLKRVDAQRAGVVVW
                     ARRPTFAGGHIFHWGRQTYVMGVVNVTPDSFSGDGLWRDAEVVERAVAQARQMLAGGA
                     HVIDVGGESTRPGASPVDAAEEQRRIAPVIEAFAREGLGPISIDTYRSETARVALDAG
                     ASIVNDVWGLRRDERLAALVAARGAPVILMHNRSKPQDAAFEARLGPRYVGSHYDNLL
                     EDVARELAESVDIARRAGIAEHRIVVDPGIGFGKTVEHNLELLDRLDALQALNKPILV
                     GPSRKSFIGYTLDVPPEERLEGTAAAVAIAIARGADIVRVHDVPPIARVARMTDAIVR
                     RARRQ"
     gene            complement(3146..3796)
                     /locus_tag="AC162G_14720"
     CDS             complement(3146..3796)
                     /locus_tag="AC162G_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077107.1"
                     /translation="MKATAAPDDTFTRAERKFLARLDSPVKIQAFLDGIPYSDEDIYR
                     CPRRVMRDRKAHCFDGALFAAAALRRLGYPPLVIDMLADNDDDHLLALYKVDGHWGAV
                     AKSNFVGLRLREPVYRTVRELVMSYFEQFYNVRREKTLRSYTVLLNLAVFDRLHWMRR
                     DEPLDAIADCLDKRRRVFVVTPAMVGRLQPVDKRSFQAGLQGANPNGLYRPPRERG"
     gene            3958..4731
                     /locus_tag="AC162G_14725"
     CDS             3958..4731
                     /locus_tag="AC162G_14725"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR5077108.1"
                     /translation="MRIIRILIDAQPAYGLVEGETVYRLDGSPFEPFTRGAPIGALES
                     VELLVPCQPTKIVAIGRNYAEHAREHQSEVPAEPLIFLKPPSALLAHRRSIILPPQSS
                     RVEHEAELAVVVGKRARHVSQADAWAYVLGVTCANDVTARDLQRSDGQWSRAKGFDTF
                     CPLGPWIETGLTPQQIESLSIVCRVNGQLRQSGHTRDMVFDIPRLIAHISAAMTLEPG
                     DVILTGTPAGVGPLVAGDTVEVEIESIGALRNSVQEPGF"
     gene            4863..5267
                     /locus_tag="AC162G_14730"
     CDS             4863..5267
                     /locus_tag="AC162G_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5077109.1"
                     /translation="MEEEIWDTLHRHLKSIFTRDTATYANTTSGDLSLYEWWVTPHRQ
                     DGLDFHLFMIENAWAGAASAWRYDLLEPRLQVYGDTAVASYTLMVSMAREDGITHRMH
                     NESRVLVKREGRWQIVHVHKSPAWPAPYQPTS"
     gene            5351..5590
                     /locus_tag="AC162G_14735"
     CDS             5351..5590
                     /locus_tag="AC162G_14735"
                     /inference="COORDINATES: protein motif:HMM:NF019844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR5077110.1"
                     /translation="MELTFEPLPQPASGSALRRVQIKRDWLRMRDAPSLQGRVLVELP
                     GGTELIVLEERGNWLRIARPTGWVNEDYVRDAGLG"
     gene            5630..6862
                     /locus_tag="AC162G_14740"
     CDS             5630..6862
                     /locus_tag="AC162G_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5077111.1"
                     /translation="MTTATQKASLRALPRNVWAVSLTSFFMDISSEMVLNVVPLFLAN
                     VLGVKTGIIGLIEGVAESTASLLKLFSGWLSDKLRARKWPAVAGYGLSALSKPFFYFA
                     SSWGAVAAVRWVDRAGKGIRTAPRDALVADSIDEQRRGLAFGFQRAADTAGAVVGLLI
                     ALGVVWLAQSSAVELGVDTFRAVVLISIVPAVLAVLTLAIGAQDVSVTKQRPAPKFAV
                     RSLGKPFVIFLIIVGIFELGNSSDAFLVLRAQERGMSVAGILAMLVAFNLVYASVSTP
                     AGLLSDRIGRRKVIVGGWLVYAAIYLGFGLAQTGWQVWMLYVVYGFYYGLAYGTSKAM
                     VADLVPEPVRGTAYGTYNAALGILDLPASVIAGLLWQGVGAWGGFGPSAPFIFGAAMA
                     LVAALSMVVWTPRGMQAE"
     gene            7045..7506
                     /locus_tag="AC162G_14745"
     CDS             7045..7506
                     /locus_tag="AC162G_14745"
                     /inference="COORDINATES: protein motif:HMM:NF018058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIR5077112.1"
                     /translation="MQIRLIAALVIALLAVAFALLNSTPVTVNLLFTQFPGSLALILL
                     GAVFVGALLSLLLSAPSTVRRRRSRSEEQKRIKELEKGIADRDATLHELEKNIAGRDA
                     KLHELEKIIAERNTKIRELEARAEARPSPPREQPEPAWPEPIQPPSDISSP"
     gene            7729..9423
                     /gene="argS"
                     /locus_tag="AC162G_14750"
     CDS             7729..9423
                     /gene="argS"
                     /locus_tag="AC162G_14750"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR5077113.1"
                     /translation="MPQLIRDQLTALIRDAIHDAQKMGALPAFDAPRIEMTRPKIAAH
                     GDYSTSAALALAKTAGMTPLQIAQHIVDHLPAHDAIGKVEIAKPGYINFTLSEVWLAQ
                     QAQAILDAGPEWANPDMGTGQRVQVEHGSANPTGPLHFGTARNFVVGDTLANILQAAG
                     YEVQREYYVNDAGSQVRKFGESIYARYAQALGQDAPFPEDGYKGEYIADEAQAIIREH
                     GDRFLRMEQAEAIRALGQIGLDAMVERARRTLARMNIRYDRWFHERSLYESGLFDRVI
                     DQLRARDLLYEQDGATWFKVSAFGGEKDAVIIRSAEVIPEPSERPTYFGSDIAYVHDK
                     FIDRDFDRVIYVWGADHHGDVPRMQAIGRAFGIAPERLTVLLYQLVTLTRGGEEVRQS
                     KRKGEFISLDEVIDEIGSDAIRFMLLTRSIDSKIALDLELAKEQSDQNPVYYVQYAHA
                     RIASILRKVVDVGVPPDAKGDPQLLKHPSELTLIRKMLELPVTIEQAARDLAPHHLAH
                     YAQDLASTFSAFYRDCRVVDPSAPDLTSARLMLARAVKLTLARVLGLMGMSAPESM"
     gene            9482..9847
                     /locus_tag="AC162G_14755"
     CDS             9482..9847
                     /locus_tag="AC162G_14755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5077114.1"
                     /translation="MAIKSYHDLEAYQRAKKLIPLIYQLVETFPADERFDLCDQMRRA
                     CKSVVDNIVEGYSHKDTPTKAKAFWRNSMGSANEMVEHLEQSVTLKYATSELAQPMIN
                     EYTIVAKQLNRLIQNWRKL"
     gene            9898..10458
                     /gene="dcd"
                     /locus_tag="AC162G_14760"
     CDS             9898..10458
                     /gene="dcd"
                     /locus_tag="AC162G_14760"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR5077115.1"
                     /translation="MTIQPDHWIRKMALEHGMIEPFVERQVSNHVISYGVSSYGYDIR
                     VADEFKIFTNVYSAIVDPKHFDPKSMVDFKGDVCIIPPNSFVLGRTVEYFRIPRNVLT
                     ICVGKSTYARCGLIVNVTPFEPTWEGYVTLEISNTTPLPAKLYANEGIAQVLFFVADS
                     ECEVSYKDKKGKYDKQTGIVLPRIFQ"
     gene            10528..10601
                     /locus_tag="AC162G_14765"
     tRNA            10528..10601
                     /locus_tag="AC162G_14765"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10562..10564,aa:Val,seq:tac)
     gene            10735..13947
                     /gene="gcvT"
                     /locus_tag="AC162G_14770"
     CDS             10735..13947
                     /gene="gcvT"
                     /locus_tag="AC162G_14770"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF012677.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR5077116.1"
                     /translation="MSDFLFHGDLAQLDPAIAELIEHEAERQARKLILVPSESAASKA
                     VREALGSVFQNIYAEGYPDPRTRAQTEPEILDYVEQLGAYRRYGDPRYYKGVEYADIV
                     EALARRRCAELFATPIIGPGRIFVNVQPLSGAPANNAVYEALLKPGDTVMGMSLVVGG
                     HLTHGSPVNRSGRHFNIVAYDVDPATEQLDYDHLRDLARQHKPKMIIAGYTSYPYAPD
                     WSKFRAIADEVGAVLLADISHVAGLVAAGAFPSPLGAAHVITFTTHKTLCGPRGACIL
                     TLDASLSRKIDRAVFPGEQGGPHVNAIAGMAVAFKLAAAEEFKQLQHQVVKNAVALAK
                     ALENNGLRIAYGGTSSHLLLVDCKSVKAKDGTPLMGDPAARILDLANIVCNRNTIPGD
                     RGAGAASGIRFGTPWVTQRGLKEADMKRLGELIAGVLKACTPFAYDARSGEAFRAKVD
                     FDVVENTKLEVAQIIAAMEGDVEAKPSGYPHFWSLADAYGAIAVFEIRGDKARSLLQQ
                     ATTNDVDALKDGDSQPTFLLEKDGRVMAGGSLKRVDAYTYHLAVMGQRGARVAAWLRA
                     LSDGFASFDDDTHAKLAGPVSVKHLAEAQTAGPLALDLEGAVAVYKPYFIGMRGENLA
                     KVLDPSTALRAGPSEGLPTLPPFSWTEAHDAPLKRTTLYETHKKMGAKMVPFAGWEMP
                     VWYTGVIEEHKAVREAAGLFDVSHMGVLDASGPNAAAFLDLITTNDVAALGIGNSHYS
                     YLLGSDGIPIDDLMIYRIQAERYLIVVNASNNDKDWAWMNAVNEGTVRIDAARPWARS
                     TAPCALRDLRDRRWGDECRVDIALQGPKSLAILQSICDNKADAARLDKMPRTDVMRAT
                     LKGFDAIISRTGYTGESVGYELFVHPDKASAFWDLLVEVGTPLGMKPIGLGARDSLRT
                     EAGLPLYGHELAGPMNMNPADAGFASYVKTYKPFFVGRAPYLAHDAGRKMENARFRMN
                     DKGVRVPKGAAALETGGDMDYVIDKRGRVIGRVLSCAVDSEGYMTGQALVEMKYTVEG
                     TPIGILPASRDAKPAADLKIGSRVPTIDPATVVSRFPKRK"
     gene            14189..16093
                     /locus_tag="AC162G_14775"
     CDS             14189..16093
                     /locus_tag="AC162G_14775"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015187387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077117.1"
                     /translation="MTAMAGYSDLTLYRRLLRQARPYWLHTAGIFLISLLASPLALLT
                     PLPLKMVVDNVIGSHPLPSFVDALLPAAIAQSGVALLILAAGLQVGITLLSQLQELGA
                     SLLRTFTGEKLVLEFRAQLFRHVQRLSLTYADSKGTADSTYRIQYDAPAIQSIAIDGV
                     IPFITAGLTIAGMIYVTARIDWELALVALAVTPILFLLTRFYRKRLRQQSREVKRLES
                     SAVSVVQEVLSALRVVKAFGQEDREEERFVRRSSEGMRARIHLTFVEGGFGLLVGLTS
                     AIGISAVLFIGALHVSEGILTLGDLLLVISYLSQLYAPLKTMSKKAAGLQSHLAGAER
                     AFSLLDEAPDVPERPNARPLVRASGDVIFHNVSFAYPAGWGVPSAPLRGSASSSEMDH
                     PILHDISFEIPAGARCGIAGATGAGKTTLVGLLTRFYDPTAGEILLDGIDLRDYKLAD
                     LRNQFAIVLQEPVLFSTSVAENIAYARPGVVERDIVAAAKAAGAHEFIVRLPRGYETP
                     VGERGMRLSGGERQRIALARAFLKDAPILILDEPTSSVDVKTEAAIMEAMGRLMRGRT
                     TFMIAHRLSTLSICDARIEIEQGRIVAISEHATSTPLERIEPIAPMQPRGQGDRERVN
                     HRRDHIHRPA"
     gene            16053..16532
                     /locus_tag="AC162G_14780"
     CDS             16053..16532
                     /locus_tag="AC162G_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077118.1"
                     /translation="MLTTAEITYTDLREHPAVRAWGELWPRWAEPERVEILKDHAKST
                     VYRLEGVGRGRTAIIAKRCRTDIAAIERTIYEDVLPSLPITTLHYYGFVEENDQFCWL
                     FLEDAGQEHFSPVIEEHRALAAQWLALMHTSHAAFGLCDRFGTPKFDIKELAGRAGE"
     gene            16799..17812
                     /locus_tag="AC162G_14785"
     CDS             16799..17812
                     /locus_tag="AC162G_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077119.1"
                     /translation="MMNTLRDVHRKIRAIASGLQSRRVSLLACGLLIGILVAACSGGS
                     PSPTPSANTPSSAPSANTPAASQATATGQSRLPGPEEFGMTTEELVTSVEAVESLISK
                     CMSDAGFEYIAVDYDTVRKGMVADKSLPGVSDDDYIAQYGYGISTLYTGLPPQLADVA
                     TPAQIGLGEQNVRIFNSLSPADQVAYNRTLFGENIDATLAVSLEAEDFSRTGGCTRAA
                     IEQVFAREQLSATYLNPLDALIEQDPRMIAALDEFASCVREEGFNYSHPNEIEPDINN
                     RLDAITKGAPLEALSAEAQAALAELQGYERAVAALATSCEEDIVEPVQAQIERELYGG
                     QQP"
     gene            17895..19382
                     /locus_tag="AC162G_14790"
     CDS             17895..19382
                     /locus_tag="AC162G_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HIR5077120.1"
                     /translation="MSRKRNLTLFIVLALVVLVAAGSWIAGSNLMTPAEAAARTAPPV
                     PSPILVPVEERVLTSDIVTRGTARYGRPQSISIVPSPLKPGGGIITTLPVRNTQLNEG
                     DVMLTASGRPVFILQGEIPAYRDLVPGSSGDDVRQLENALKRLGFDPGPVDGSFDEET
                     STAVADWYASAGWEPFGPTPEQLANIRALEQELAVGISNRSAAYAAAAAAPLAVEAAR
                     ANADSANQAAAADVADKTRLRDWATDEDRAKANVDLEVARAGATATRLAGEMAVQAAV
                     DAQQAAEREAKLAADSVVRLAADLEIARRKAGVQVPADEIVFIPAVPVRVEQINVVVG
                     EAPSGPVMTVTNYQLAIDSSLALDEAPLVKPGLPVAIDEPDLGIKATGVVGRVADTPG
                     TFGVDGFHIYLEVLVDETPVALEGFSLRLTIPVKSTGGMVTVVPVSALSLAADGTSRV
                     QVERNGSLEFIAVEPGLSADGFVEVTPVDGALAPGQLVVIGFEQQ"
     gene            19379..21343
                     /locus_tag="AC162G_14795"
     CDS             19379..21343
                     /locus_tag="AC162G_14795"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HIR5077121.1"
                     /translation="MMTTTQPNTTKYVVELHKVSRTFGSEPAVHALVEVDLWLEHGEW
                     LAITGPSGAGKSTLLHIIGCLDRPTSGSYALDGIDTATLRDKERAGLRSRRIGFVFQS
                     FHLLPHRSVLENVMLAEVYRKESHRGRRDRALAAIARVGLSQRADFLPTKLSGGEKQR
                     VAIARALVGSPSLLLCDEPTGNLDSKSSADLLDLFESLNRQGLTLVVVTHDEKVANRA
                     SRRVHIIDGSLTDVTNGENNRKLSPSPSTSVKSSAVSRSGITIRDLLEEAIAGMFARP
                     WRMVLTMLGIVIGLTALVATLGLSATANNRIISQFDELAATGIVVTARPGPAGVDPKA
                     LPWDAPARLGRLNGVVAAGTLSEVNIGDTLVSSSPVKDPRSQTAFKLSVKAASPDLFT
                     AVRADLETGRFPDLGHSDRVDRVAILGPGAALRLGIVSLEQLPAIAIGDKLYLVIGIL
                     RGVARQPDLLGSVIIPEGTARHDFGLAGPGSVIVETRIGAASLIVRQTPLALRPDNPR
                     VIRVELPPEPQRVREAVQGDLNVTFLLLGGLSLIVGAIGIANIMLVSVMERIGEIGLR
                     RAIGATRGHIAAQFLLESASLGALGGILGASLGVLVVVAVSAFQAWAPVLDPAAPVLA
                     PLVGGGIGLVSGTYPALRAARMEPVEAFRN"
     gene            21340..21543
                     /locus_tag="AC162G_14800"
     CDS             21340..21543
                     /locus_tag="AC162G_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077122.1"
                     /translation="MNLTEPATSQVERLADKRYVTLILRLRIDRRERLVHGEVVDTEG
                     KSQGRFAGWREMTRTVRNYLMSH"
     gene            21630..22310
                     /locus_tag="AC162G_14805"
     CDS             21630..22310
                     /locus_tag="AC162G_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5077123.1"
                     /translation="MRILIVEDQPNVSNYVKRALEEQGYAVDLARTGREALDWAEVVE
                     YDLIVLDIMLPEMDGITVCRRLRSQGHPAAILMLTARDTVDDRVTGLDAGADDYLVKP
                     FSLKELLARLRALARRRTAKTSTLQVANLSLDTRTRVVMRSNTFIKLTAKEYAILECL
                     MREPGRVLTRTEIAESVWNYDAYNQSNVVDVYIRNLRRKIDDAFDVKLIHTVRGAGYC
                     LSDQDSDA"
     gene            22307..23704
                     /locus_tag="AC162G_14810"
     CDS             22307..23704
                     /locus_tag="AC162G_14810"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5077124.1"
                     /translation="MKRLKTLRVRFALWTAGLLFAALTLFGLFVYANMSRSLAAAVDE
                     KLRLVVVQLVAEVDVEGGEPVINENPIEDPEYAQLREQGFSMRLLDLTGQVVQEFGPY
                     RDLPQPQLSFIVPEQPGEFTTLIDAGSQDPVRVYTTPIVVEEELVGTLQVAQNLKEVR
                     RTLDLLLITLLLGGPLIVIVAGSGGYFLAARALAPIDKITRMARYISAEELSARLNLP
                     ETDDEVGRLAATFDSMLARLDDAFRRERQFTADASHELRTPLSAIHTIIGSTLARRRA
                     PAEYEQAMIDLGQEAERMRTLTEGLLDLARLDATRQPAKFERVDLSNLLKDVVDSLRP
                     LAEDQGLKLIDHVPDNGLTLMGDSDGLIRLFVNLLDNAIKYTEQGSITISAHPTSEQL
                     LEVTIRDTGVGMTPEHLTRIFDRFYRVDASRSKDGIGLGLAIVKNAARAHGGKVSVES
                     KFGEGTTFIVQLPTK"
     gene            complement(23815..>24463)
                     /locus_tag="AC162G_14815"
     CDS             complement(23815..>24463)
                     /locus_tag="AC162G_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077125.1"
                     /translation="EDGETFAHRIEFTVTDLTKEIEGVRTVVAWVVDYSDGEVVEKEI
                     AFYAQDNDGNVWYLGEYPEEYEGGEFVKAPTWIAGLEDARAGIKMWAEPQPGMPSYFQ
                     GWGPAVEWTDFAQVDQMGQETCVPVDCYEDVLVIAESSLDELGAFQLKYYARGVGEVR
                     VGWRGDDATQEDLELVELVQLDPEALAEVRAEALELEKHAYEISEDVYAHTSPAE"
BASE COUNT         4695 a         7678 c         7507 g         4583 t
ORIGIN      
        1 caacctcagc gaccttcctg ccaaagcctt caggcttgcc tttcaagaat gatattcgta
       61 caagtggcat ggattacccc tcccttgtcg cacctcgatc ggtgaaccga atacagagat
      121 cgcctccccg gcgacgggca tcactggccg ggacacacga tctcccgcac gctgtccagg
      181 tgcagcagaa tggagctggt ggcgacgtcg tccggcatgt cgttgacgtc gacctccgga
      241 tcagacgcga agaggaattc cacgccttcg tacacgtcgg cttcgggatc ggccggcaca
      301 tactccagcc ccaccagata caggccggcc agctcgtcta acagctcatt ttcgtcggtg
      361 ccgatcgggt cgccgcagtg cgggcacaac agaggcagct cgtgatcgtc ctcgaacaag
      421 acggtgatca ggccgtcctc gacgacgacg aggtccttga cctgcttgcc gccgaggtgc
      481 gcgtcgatgt gctccagcgc gcacacggtc tcgtcgtcga tctgaatcat ggggcagtcg
      541 tgctcggcgc atcgcgccga agtgtggcgg ggtctgcgtg gcatgccgta tcgctccttc
      601 agcccatgag ctcttcgatc gcgtcgtcca gccgctgctg cggcccggcc atcactccta
      661 ccaccgatcg ccgcgcggcg gcctgtcgtc atcgtcgatg gtcgggatcc acaggtcgtc
      721 acgcgggagc gggcggcctc gacggctcgg cgcgaacaag cggacgagca ggaaaccggc
      781 cacgaatcca ccgatgtgag cgaaaaatgc cacgccaccc gtccgcacca ggccaagcgt
      841 tccgatcccg ctcaacaact gcatcaagaa ccagaatccc aacagccagc cggccggcag
      901 ctcgacgaag cgcacgagga agaacaagaa tacgagtgtg aggacgcgcg cattcgggaa
      961 caagaggatg tacgcgccga gcacgccgga gatcgcgccg cttgcgccga tcatcgggat
     1021 ttcggacgat gggttgatcg cgatctgagc cgccgacgct gcaagtccgc acactaaata
     1081 gaacaacggg aaacgcacgc tgcccaggcg gtcctcgatg ttgtttccga atatccacag
     1141 gtatagcatg ttgctgagga gatgcaacca gccgccgtga agaaacatcg acgtgacaag
     1201 cgtgtgcgcg tacgcgccga agtcggcggt aatcagcgcc ggcgtcacag catagctgaa
     1261 aatgaacccg gtgtaggatc gctccggcaa cgattcctga aacaggaaaa cggcgacgtt
     1321 ggccacgatc agggcgaccg tcacaatggg cgtgtgtcgg gtcggattgg cgtcgcgcag
     1381 cggtatcata gaatggcctt tccaggcaaa gcgcacccta ttataatcac agtgatgagc
     1441 gagtcacccg agatcgcccc ggccatgccc gatatcggcg tcgagttcac ctacgacgag
     1501 gcggctgaac tcgacccgcc ccacgacgtg atcgtccaca acgacgacgt gacgccgttc
     1561 gacttcgtcg tggccgtcat tcacagcgtc ttcgagctgc cgccgcacga cgcgtatgag
     1621 gtgacgctga ccgcccacta cgaaggcgag gcctacgtcg ccacgctgcc catagaggac
     1681 gccaagtacc gcgtgtaccg ggcgcacagc gctgcgcgcg ctcgcggcta cccgctgacc
     1741 ttcagcatcc accccgctca ctgacgccgc gcgcgccgca cgatcgcgtc agtcatgcgc
     1801 gccacgcgcg ctatcggcgg gacgtcgtgc acgcggacga tatctgcacc gcgcgcgatc
     1861 gcgatcgcca cggccgccgc cgtgccctcc aggcgctcct caggcggcac atccaaagta
     1921 tacccgataa acgatttgcg cgacgggccg accagaatcg gcttgttcag cgcctgcagc
     1981 gcgtcgagcc ggtcgagcag ctccaggttg tgctcgacgg tttttccgaa gccgatgccg
     2041 ggatccacga cgatgcgatg ttccgcgatt cccgcgcgcc gcgcgatgtc gaccgactcg
     2101 gccagctcgc gcgccacatc ttcaagcagg ttatcgtaat gcgacccgac gtaacgcggt
     2161 ccgagccgcg cttcgaaggc ggcgtcctgc ggcttgctgc gattgtgcat gaggatcacc
     2221 ggcgcgcctc gcgcggcgac cagcgcggcg agccgctcgt cgcgccgcag cccccacacg
     2281 tcgttgacga tcgacgcgcc agcatccagc gcgacgcggg ccgtctcgct tcggtaagtg
     2341 tcgatcgaga tcgggccgag accctcgcgc gcgaacgcct cgatcaccgg cgcgatccgg
     2401 cgctgctcct cggcggcgtc gaccggcgat gcaccgggcc gcgtcgactc tccgccgaca
     2461 tcgataacgt gcgcgccacc ggccagcatc tggcgtgcct gggcgactgc cctctcgaca
     2521 acttcggcgt ctcgccacag gccgtccccg gagaacgaat cgggcgtgac gttgacaacc
     2581 cccatcacgt acgtctggcg gccccagtgg aatatgtggc cgcccgcgaa tgtcggccgg
     2641 cgcgcccaca ccaccacacc cgcccgctgc gcgtcgacac gcttgagcag ctcgcgcacg
     2701 ctttcgccaa gcacgggatg cacagcatcc ggggcaattt cacacaacgg cgccagcacg
     2761 aaggcgcgct cggccatgcg cggatgcggc accgtcacag cgggagcttc gacgacctcg
     2821 tcgccgtaca gcagcaagtc gatgtcgatc aggcgcggcc catagcgaac ggcgggccgg
     2881 cgtcccatat cggcctcgat cgccttaagc cagacgagca aatcgcgcgg cgatagatcg
     2941 gtctggatgg cgcacacgct gttcagaaag cgcggttgat cggccacgcc ccatggctcg
     3001 gtctcgtata ccgaggagag atcgaccagg cccgcccgcg cacgcaggcg atcgatcgcg
     3061 tcgatcaggt tgccgtgccg atcgtccagg ttgctgccca gacccagata ggctcgcgcg
     3121 ctcatgaatc cgacatctcg cccggtcagc cgcgttctcg cggcggtctg tacaacccgt
     3181 tcggattggc gccctgcagc cccgcctgga acgaccgctt gtcgaccggc tggagccggc
     3241 cgaccatggc cggcgtcacg acgaacaccc gtcgccgctt atccaggcaa tccgcgatag
     3301 cgtccagggg ttcgtcgcgc ctcatccagt ggagcctgtc gaagacagcc aggttgagaa
     3361 ggacggtgta actgcgcagc gtcttctcgc gccgcacgtt gtagaactgc tcgaagtagg
     3421 acatcaccag ttctcgcacg gtgcggtaca ccggctcgcg aaggcgcagg ccgacgaagt
     3481 tggacttggc cacggcgccc cagtggccgt cgaccttgta gagtgccagc agatgatcgt
     3541 cgtcgttgtc ggccagcatg tcgatgacga gcggtggata tccgagtcgt cgcagcgcgg
     3601 cggcggcgaa cagcgcgcca tcgaagcagt gcgccttgcg atcgcgcatg acacggcgcg
     3661 ggcagcggta gatgtcctcg tcgctgtacg gtatgccgtc caggaaggcc tgaatcttaa
     3721 ccggactgtc cagccgggcc aggaactttc gttcggcgcg tgtgaaggta tcatcgggcg
     3781 ctgctgtcgc tttcatcgat cactcccagg tcatttaagt ctactcgcat acgggagaaa
     3841 ggcaagagcg cgagtgaccg gctcagctca tgcgattgtc gcggcgcgac acgcagcttg
     3901 tattgtttct gacgcgctct gtgctacaat gtttgtactt ccaccagcgg aggctctatg
     3961 cgcattattc gcatcctgat tgacgcccag cccgcatatg gactggtcga gggcgagacc
     4021 gtgtatcgac tggatggctc gccgtttgag ccgttcacgc gcggcgcacc gatcggcgcg
     4081 ttggagtcgg tcgagctgct ggtgccctgc cagccgacca agatcgtggc catcggacgc
     4141 aattacgccg agcacgccag agagcaccaa tccgaagtcc cagccgagcc gttgatcttc
     4201 ctgaaaccgc ccagcgcgct cctcgcgcac cggcggtcaa tcatcctgcc gccgcaatcg
     4261 agcagggtcg agcacgaggc cgagctggcc gtcgtcgtcg gaaagcgcgc caggcatgtg
     4321 agccaggctg atgcctgggc ctacgtgttg ggtgtgacgt gcgccaacga cgtaaccgcg
     4381 cgcgatctgc agcgcagtga cgggcagtgg tcgcgcgcca aggggttcga tacgttctgc
     4441 ccgcttggcc cgtggatcga gacggggctc acgccgcagc aaatcgagtc gctgagcatc
     4501 gtgtgtcggg tgaacggcca gcttcgccag tctggccaca cgcgcgacat ggtcttcgat
     4561 atcccacgcc tgatcgcaca catttcggca gcgatgacac tcgagccggg cgacgtgatc
     4621 ctcaccggca cgccggcggg tgtcggcccg ctggtcgcgg gcgacaccgt cgaagtcgaa
     4681 atcgaatcga tcggcgcgct gcgcaacagc gtccaggaac ccggtttcta gagcgatttc
     4741 cccttggatt tgtgacctgg aatcgaggct ttagccggat ccgcccaaag gctcgacccc
     4801 ggaagctagt aaacgaaaac ggaaacccct ttagaagaaa cggatttctg ccgggaggtc
     4861 ttatggaaga agagatatgg gatacgctcc accgccacct gaagagcatt ttcacgcgcg
     4921 acacggccac ctatgccaac acgacgtccg gcgacctcag cctgtacgaa tggtgggtta
     4981 cgccgcaccg ccaggacggg cttgattttc atctgttcat gatcgagaat gcgtgggccg
     5041 gcgcggccag cgcctggcgc tacgacctgc tggagccacg ccttcaggta tacggcgaca
     5101 ctgccgtcgc ctcgtatacg ttgatggtca gcatggctcg cgaagacggc atcacgcatc
     5161 gcatgcacaa cgaaagccgg gtgctggtca agcgcgaggg gcgctggcag atcgtgcacg
     5221 tgcacaaatc gccagcctgg cccgcgccgt atcaacccac atcctaaaga cacactctcc
     5281 cgaactggag tttcgcaaca gccataccta tactgaacaa ggagagaagg aacattctcg
     5341 gaggctgtac atggaattga cattcgagcc gctgccccaa ccggcatccg gcagtgcgct
     5401 gcggcgcgtc caaatcaagc gcgactggct gcgcatgcgc gatgcgccgt cgctgcaagg
     5461 ccgtgtcctg gtggagctgc ccggcggcac ggagctgatc gttctggaag agcgcggcaa
     5521 ctggctgcgc atcgcccggc caacgggttg ggtgaacgaa gactacgttc gggatgcagg
     5581 gctgggataa cacgattcca cctgaaaacc tgccagcgcg aggcactcca tgactaccgc
     5641 aacgcagaag gcgtctctcc gagcgctgcc gcgcaacgtg tgggcggtca gcctgacgag
     5701 cttcttcatg gacatttcca gcgagatggt gctcaacgtc gtgccgctgt tcctggcgaa
     5761 cgtgctgggg gtaaagaccg gcatcatcgg cctcatcgaa ggtgtggcag aatcgacggc
     5821 cagtctgctc aagctgtttt cgggctggct ttccgacaag ctgcgcgcgc gcaagtggcc
     5881 ggcggtcgca ggctatgggc tgtcggcgct ctcgaaacct ttcttctact tcgcaagcag
     5941 ttggggcgca gttgccgccg tgcgctgggt cgaccgggcc ggcaaaggca tccgcaccgc
     6001 cccccgcgac gcgctcgtgg ccgactcgat cgacgagcag cgccggggcc tggcgttcgg
     6061 ctttcagcgc gccgccgaca cggccggcgc cgtcgtcggg ctgttgattg cgctcggcgt
     6121 cgtgtggctg gcgcagtcga gcgcggtcga gctgggagtc gacacgttcc gcgccgtcgt
     6181 gctgatcagc atcgtcccgg ccgtgctggc ggtattgacg ctcgcgatcg gcgcgcagga
     6241 tgtgtcggtc acgaaacaac gtcccgcgcc gaagttcgcc gtgcgatccc tcggcaagcc
     6301 tttcgtcatc ttcctcatca tcgtcggcat tttcgagctg ggaaactcct cggacgcgtt
     6361 cctggtgctg cgcgcgcagg agcgcggtat gagcgtggcc ggcatcctgg cgatgctggt
     6421 cgcattcaac ctggtatacg catcggtctc cacgccggct ggattgctct cggatcgcat
     6481 tgggcgacgc aaggtgatcg tcggcgggtg gctcgtctat gccgcgatct atctcggctt
     6541 cggactcgcg cagaccggat ggcaggtatg gatgctgtac gtcgtgtatg gcttctacta
     6601 cggattggcg tacggcactt ccaaagcgat ggtagccgac ctcgtgcccg aaccggtacg
     6661 aggaacggcg tatggcacgt ataacgcggc tctgggaatt ctcgaccttc cggcgtccgt
     6721 gattgccggt cttctgtggc agggagtcgg cgcgtggggc ggattcggcc catctgcgcc
     6781 attcattttc ggcgcggcga tggcgctggt tgcggcgctg tcgatggtcg tctggacgcc
     6841 gcgtgggatg caggccgaat aacgcgagtg gccgcgacat cacttgccat ccggcgcgtc
     6901 ggcgcgtaca atgtccgcca agacttcgga agtctcacaa tgccatcttg ctcgcgggat
     6961 gtctcggatt gcctgacatc ttcgcccgcg agaattagcc ttaagacttc cgaagtctga
     7021 gaccagattc aggagagaca accgatgcag atacgattga tcgctgccct ggtcattgcc
     7081 ctgctggccg tcgccttcgc acttctgaac tccacgcccg tgacggtgaa tctgctcttc
     7141 acgcagttcc ccggatctct ggcactgatt ctgctcggcg cggtcttcgt cggtgcgctg
     7201 ctcagtcttc tgctgtccgc gccttcgacg gtccggcgac gccgctcgcg atcggaagag
     7261 cagaagcgga taaaggagct ggagaagggc attgccgatc gagacgcgac gctgcacgaa
     7321 ctggagaaga acatcgccgg gcgagacgcg aagctgcacg agctggagaa gatcatcgcc
     7381 gagcgaaaca cgaagatacg ggaactcgaa gcccgcgccg aggcacgccc atccccgccg
     7441 agggagcagc ccgagccggc ctggcctgag cctatccaac caccatccga tatatcctca
     7501 ccttagagcg gattccgagt gcgtgtacgc cgatccggag tcgagccttt gggtggtttc
     7561 cggttgaagc ctcgactccg gcttaaatca aagtgcaacc cgctctagtg cagcgcggca
     7621 gaaatgcctc gccagttttt catgcgccgg ttttttcttg tcggatcacc ctttggcagg
     7681 tagaatagct tctcgcaggc gtgaagcgtg aacctgcggg aggctattgt gccccaactc
     7741 atccgcgatc aacttaccgc gctcatccgt gatgcaatcc acgatgcaca gaagatgggc
     7801 gccctacccg ccttcgacgc gccgcgcatc gagatgactc gacccaagat cgcggcccac
     7861 ggcgattact ccacgagcgc ggcgctggcg ctggcgaaga cggcgggcat gacccccttg
     7921 cagatcgcgc agcacatcgt cgatcacctg ccggcccacg acgccatcgg caaggtcgag
     7981 atcgccaaac cgggctacat caatttcaca ctctcggaag tgtggctagc gcagcaggcg
     8041 caggcgattc tcgacgccgg ccccgagtgg gccaaccccg acatgggcac cgggcagcgc
     8101 gtgcaggtcg agcacggatc agccaacccg accgggccgc tccacttcgg cacggcgcgc
     8161 aacttcgtcg tcggggacac gctcgccaac atcttgcagg cggccggtta cgaggtgcag
     8221 cgcgagtact acgtgaacga cgccggatcg caggtgcgca agttcggcga gtcgatctac
     8281 gcgcgctacg cccaggcgct gggccaggac gcgccctttc ccgaggatgg ctacaaaggc
     8341 gagtacatcg ccgacgaggc gcaggcgatc atccgcgagc acggcgaccg attcctgcgc
     8401 atggagcagg ccgaggccat ccgcgcgctg ggccagatcg gcctggacgc gatggtcgag
     8461 cgcgccaggc ggacgctcgc ccgcatgaac atccgctacg atcggtggtt ccacgagcgg
     8521 tcgctgtatg agtccgggct gttcgaccgg gtcatcgatc agctccgcgc gcgcgacttg
     8581 ctgtacgagc aagacggcgc gacgtggttc aaggtgagcg cattcggcgg cgaaaaagac
     8641 gccgtgatca ttcgatcggc cgaggtcatc cctgagccga gcgagcggcc cacgtacttc
     8701 ggcagcgaca tcgcttacgt tcacgacaaa ttcatcgacc gggacttcga ccgggtgatc
     8761 tacgtgtggg gcgcggatca ccacggcgac gtgccgcgca tgcaggcgat cggccgggca
     8821 ttcggcatcg cgccggagcg gctaaccgtc ctgctctatc aactggtgac gctcacgcgc
     8881 ggcggcgaag aggtacggca gtcgaagcgc aaaggcgagt tcatctcgct cgacgaggtc
     8941 atcgacgaga tcggctccga cgcgatccgc ttcatgctgc tcacgcgctc catcgacagc
     9001 aagatcgcgc tcgacctcga gctggcgaaa gagcagtcgg accagaaccc ggtgtactac
     9061 gtgcagtacg cgcacgcgcg catcgccagc atcctgcgca aagtggtgga cgtcggcgtg
     9121 ccgccggacg ccaagggcga cccacaactg ctgaagcatc cctccgagct gacgctgatc
     9181 cgaaagatgc tcgagctgcc ggtcaccatc gagcaggcgg cgcgcgatct cgcgccgcat
     9241 catctcgcgc attacgccca agacctggct tcgaccttca gcgccttcta ccgcgattgc
     9301 agggtcgtcg atccgtctgc acctgacctg acgtccgccc gattgatgct ggcgcgcgca
     9361 gtgaagctga cgctggcgcg cgtcctggga ttgatgggga tgagcgcgcc agagtcaatg
     9421 tagagattgg aggttagaag ttagaggttg gacgttagaa gttggaagtt ggagggcgcc
     9481 gatggcgatc aagagctatc atgatctgga agcgtaccag cgcgctaaga agctcattcc
     9541 tctgatctac caactggtgg agacgtttcc ggcagacgag agattcgacc tgtgcgatca
     9601 aatgcgtcgt gcctgcaaat cggtcgtgga taacatcgtt gagggttaca gccataaaga
     9661 cacgccgaca aaggccaaag cattctggcg caattcgatg ggttcggcta acgagatggt
     9721 agaacacttg gagcagtcgg tgacactcaa gtacgcaacg tctgaactcg cccagccaat
     9781 gatcaacgaa tacaccatcg tcgccaaaca gctcaacaga cttatccaaa actggcgcaa
     9841 gctgtgagag gacagcctcc aacctccaac ctccaacctc caacttccag aggagccatg
     9901 accatccaac ccgaccactg gatccgcaag atggcgctcg aacacggcat gatcgagccg
     9961 ttcgtcgaga ggcaggtcag caaccacgtc atttcttacg gtgtctcgtc gtacggctac
    10021 gacattcgcg tcgccgacga attcaagata tttacgaatg tgtattcggc catcgtcgat
    10081 cccaagcact ttgacccaaa gtcgatggtc gatttcaagg gcgacgtgtg catcatccca
    10141 cccaactcct tcgtcctggg ccgcacggtc gaatacttcc gcatcccacg caacgtgctc
    10201 acgatctgtg ttgggaaatc gacatatgcg cgctgcggct taatcgtgaa cgtgacccca
    10261 tttgaaccga cctgggaagg ttatgtcacg ctcgagatca gcaacaccac ccccctaccc
    10321 gccaagctct acgccaatga aggcatcgcc caggtgctct tcttcgtcgc cgattccgag
    10381 tgcgaggtct cgtacaagga caaaaagggc aagtacgaca agcagacggg catcgtgctg
    10441 ccgaggatct ttcagtgagg agcgtgctca tgtcatactt gccggcctct ggcagaaacg
    10501 gggtttccag agtaaaatag cctcaacggg cgaatagctc agttggtgag agcgcctctc
    10561 ttacaaagag gaggtcgtag gttcgagtcc tatttcgccc actccatttc agacctgcca
    10621 ggtctgcgag acctggcagg tctttcgatt atcctttgac cccgcgccct cccagtgcta
    10681 caatcttctt gcaactctaa ctccctactc tcactctctc ctcgaggagc aaccatgtcc
    10741 gatttcctct tccacggcga cctggcccaa ctcgatccgg ccatcgccga gttgatcgag
    10801 cacgaagccg aacgccaggc gcgcaaattg atcctggtcc cgagcgagtc cgccgcgtcg
    10861 aaggcggtgc gcgaagcgct gggttccgtc tttcagaaca tttacgccga gggctatccc
    10921 gacccgcgca cccgcgccca gaccgagcct gagattctcg actacgtcga gcagctcggc
    10981 gcctatcggc gctacggcga cccgcgctac tacaagggcg tcgagtacgc cgacatcgtc
    11041 gaggcgctgg cgcgccgccg ctgtgccgag ctgttcgcca cgccgatcat tggccccggt
    11101 cgcatcttcg tcaatgtcca gccgctgtcg ggtgcgccgg ccaacaacgc cgtgtacgaa
    11161 gcgctgctca agccgggcga taccgtcatg ggcatgtcgc tcgtcgtcgg cgggcacctg
    11221 acgcacggat cgccagtcaa ccgctcgggc aggcatttca acatcgtcgc atacgacgtc
    11281 gatccggcga ccgagcagct cgattacgac caccttcgcg atctcgcgcg gcagcacaag
    11341 ccgaagatga tcatcgccgg gtatacgtcg tacccgtatg cgcccgactg gtcgaagttc
    11401 cgcgccatcg ccgacgaggt cggcgcggtg ctgctggccg atatctcgca cgtcgccggg
    11461 ctggtcgcgg ccggcgcgtt cccctcgccg ctcggcgcgg cgcacgtcat caccttcacg
    11521 acgcacaaga cgctgtgcgg cccgcgcggc gcgtgcatcc tcacactcga cgcgtcgctc
    11581 tcacgcaaga tcgaccgggc cgtattcccc ggtgagcagg gcggcccgca cgtcaacgcg
    11641 atcgccggga tggccgtcgc cttcaagctc gcggccgccg aggagttcaa gcagttgcag
    11701 catcaggtag tcaagaatgc cgtggcgctg gcgaaggcgc tcgaaaataa cgggctgcgc
    11761 atcgcctacg gtggaaccag ttcgcatctg ctgctggtcg actgcaaatc ggtcaaagcg
    11821 aaagacggca cgccgttgat gggcgatccg gcggcgcgca tcctcgatct ggcaaatatc
    11881 gtctgcaacc gcaacacgat cccgggcgat cgtggcgcgg gggcggcgtc gggcatccgg
    11941 ttcggcacgc cgtgggtcac gcagcgcggg ctgaaagaag ccgacatgaa gcggctcgga
    12001 gagctgattg ccggcgtgtt gaaggcctgc acgccgttcg cgtacgatgc cagaagtggc
    12061 gaagcttttc gcgccaaggt cgacttcgac gtggtggaga acacaaagct cgaagtggcg
    12121 cagatcattg ctgcgatgga gggcgacgtc gaggcgaagc cgagtggtta tccccatttc
    12181 tggtcgctcg cagatgccta cggcgcgatc gccgtattcg agattcgcgg cgacaaggcg
    12241 cgctcgctcc tgcagcaggc gacgacgaac gacgtcgacg cgctgaagga tggcgactcg
    12301 cagccgacgt tcctcctgga aaaggacggc cgggtgatgg ccggcggttc cttgaagcgc
    12361 gtcgacgcgt acacgtatca cctggccgtg atgggccaac gcggggcgcg cgtcgccgcg
    12421 tggctgaggg cgctgtccga cggattcgca tccttcgacg acgatacgca cgccaagctg
    12481 gccggcccgg tgtcggtgaa gcacttggcc gaagcgcaga cggccggtcc gctcgcgctc
    12541 gatctcgaag gtgcggttgc agtctacaaa ccgtatttta tcggcatgcg aggtgagaac
    12601 cttgcgaagg ttttagaccc ttcgactgcg ctcagggcag gcccttcgga aggtttgccc
    12661 accctacccc cctttagctg gaccgaagcc cacgacgcgc cgctcaagcg cacgacgctg
    12721 tacgagacgc acaagaagat gggcgcgaag atggtgccct tcgccggatg ggagatgccg
    12781 gtgtggtaca ccggcgtgat cgaagagcac aaggccgtgc gcgaggcggc gggcttgttc
    12841 gacgtcagcc acatgggcgt gctcgacgcg tccggcccga acgccgctgc tttcctcgat
    12901 ctgatcacca ccaacgacgt ggccgcgctg gggatcggca actcgcacta cagctacttg
    12961 ctcggctccg acggcatccc gatcgacgac ctgatgatct accggatcca ggcagagcgg
    13021 tacctgatcg tcgtcaacgc cagcaacaac gacaaggact gggcgtggat gaacgccgtc
    13081 aacgagggca cggtgcgcat cgacgccgcg cggccgtggg cgcgcagtac cgcgccgtgt
    13141 gccttgcgcg atttgcgcga tcgccggtgg ggcgacgagt gccgcgtcga catcgcgctg
    13201 cagggtccca aatcgttggc aatcctccag tcgatttgcg ataacaaagc cgatgcggcc
    13261 agactcgaca agatgccgcg caccgacgtg atgcgcgcga cgctgaaagg gttcgacgcg
    13321 atcatctcgc gcaccggata taccggtgaa tcggtcggct acgagctatt tgtccatcca
    13381 gacaaagcgt cggccttctg ggatctcctg gtggaagtcg ggacgccgct ggggatgaag
    13441 ccgatcggcc tgggcgcgcg cgacagcctg cgcacagaag ccggcctgcc gctgtacggc
    13501 cacgagctgg ccgggccaat gaacatgaac cccgccgacg ccgggttcgc ctcgtacgtc
    13561 aagacctaca agccgttctt cgtcgggcgg gcgccgtacc tggcgcacga cgccgggcgc
    13621 aagatggaga atgcccgctt ccgcatgaac gacaagggtg tgcgcgtgcc gaagggcgcg
    13681 gcggccctcg aaaccggcgg cgacatggac tacgtcatcg acaaacgggg gcgtgtgatc
    13741 ggccgcgtac tgtcgtgcgc ggtcgacagc gagggctata tgaccggcca ggcgctggtc
    13801 gagatgaagt acacggtaga aggaactccc atcggcatct tgcccgcatc cagagacgcc
    13861 aaaccggcgg cggacttgaa gatcggctcg cgtgtgccga cgatcgaccc ggcgacggtg
    13921 gtcagccggt ttccgaagcg gaagtaatgg acgcaatact cgcagcggga aaggtaactg
    13981 ctcagccact tgtggcgtgt cattccgaac gtagtgagga atctctactc cgtcgggtgt
    14041 cgggtgtcag gtgtcaggtg tcggatagtg tcattccgaa cgcagtgagg aacctcgtcc
    14101 gaccggcgag cgcggcgcgc cggggtcgag atgcctcgtc gttcctcctc ggcatgacac
    14161 gtctggcgtg gccagagttg cgagaggcat gacggccatg gccggctatt ccgatctgac
    14221 cctgtaccgg cggctgctgc gccaggcgcg gccttattgg ctccataccg caggcatctt
    14281 cctgatcagc ctgctcgcaa gccctcttgc gcttctgacc cctttgccat tgaagatggt
    14341 cgtcgacaac gtgatcggct ctcacccgct tcccagtttt gtggatgcgc tgctgccggc
    14401 ggccatcgct caatccggcg tcgccctgct catactcgca gccgggcttc aggtgggtat
    14461 cacgctattg agccagcttc aggagctggg agcctccttg ttgcgcacct tcacgggcga
    14521 gaagctggtg ctggagttcc gggcgcagtt attccggcac gtccagcgtc tctcgctcac
    14581 gtacgccgac tcgaaaggca cggccgactc cacataccgc attcaatatg acgcgcctgc
    14641 catccagagt attgcgatcg acggcgtcat ccccttcatc acggcgggtt tgacgatcgc
    14701 cgggatgatc tacgtcacgg cccgcattga ctgggaattg gccctggtcg cgctggcggt
    14761 tacacctatt cttttcttgc ttacacgctt ttatcgaaaa cgcctgcgcc agcagtcgcg
    14821 tgaggtgaag cgactcgaaa gctccgccgt gtcggtcgtg caggaggtgc tgtccgccct
    14881 gcgtgtcgtg aaagcgttcg gacaggagga tcgcgaagag gagcgcttcg tgcgccgctc
    14941 cagtgaaggc atgcgtgccc gtatccacct cactttcgtc gaggggggct tcggcctcct
    15001 cgtcgggctg accagcgcga tcgggatttc ggccgtcctc ttcatcggtg cgctccacgt
    15061 ctcggagggc atcctgaccc tgggcgatct cctcctggtg ataagctatc tgtcgcagtt
    15121 gtacgcgccc ttgaagacga tgagcaaaaa ggcggcggga ctgcaatcgc acctggccgg
    15181 cgccgagcgg gcattttctc ttctcgacga agcaccggat gtgcccgaac ggccgaacgc
    15241 ccggccactc gtgcgcgcct ccggcgacgt tattttccac aacgtctctt tcgcctaccc
    15301 cgctgggtgg ggcgtcccct ccgctccgct acgaggatct gcgtcaagca gcgagatgga
    15361 ccatcctatc ttgcacgaca tatccttcga gatccctgcc ggggcacgat gtggtatcgc
    15421 cggcgcgacg ggcgcgggca agaccacgct cgtgggcctg ttgacccgat tctacgatcc
    15481 cacggcgggc gagatcctgc tcgacggcat cgatctgcgc gactacaagc tggccgacct
    15541 gcgcaaccag ttcgccatcg tccttcagga gccggtgctc ttctcgacca gcgttgctga
    15601 gaacatcgct tacgcgcgtc ctggcgttgt tgaacgcgac atcgtcgcgg ccgcgaaggc
    15661 ggccggcgca cacgagttca tcgtccgcct gccccgtgga tacgagacgc cagtcggcga
    15721 gcgcggcatg cgcctttcgg gtggcgagcg ccagcgcatc gcgctggccc gcgcctttct
    15781 caaggacgct cccattctga ttcttgacga gccgaccagc tccgtcgatg taaagacgga
    15841 ggccgcgatc atggaagcca tgggacgcct catgcgcggc cgcaccactt ttatgatcgc
    15901 ccaccgattg agcacgctga gtatctgcga cgcacgaatc gagatcgagc aaggtcgaat
    15961 cgtcgccatc tcagaacatg ccacatcgac gccactggag cgcattgagc cgatcgcacc
    16021 gatgcagccg cgaggacagg gggaccgcga acgtgttaac caccgccgag atcacataca
    16081 ccgacctgcg tgaacacccc gccgtccggg cgtggggcga actctggcca cggtgggccg
    16141 agccggagcg cgtcgaaatc ctgaaagatc acgcgaagtc gacggtatac cggctcgagg
    16201 gtgtcgggcg tggaaggacg gcgattatcg ccaagcgctg ccggacggat atcgcggcga
    16261 tcgaacgcac catttatgaa gatgttctcc ccagcctgcc cataactact cttcactact
    16321 atggattcgt ggaggaaaat gatcaattct gctggctctt tctggaggac gcggggcaag
    16381 agcacttttc gcccgtgatc gaggagcatc gcgcgctcgc cgcgcagtgg ctggctctaa
    16441 tgcatacctc tcacgccgcc tttggactgt gcgatcgctt tggcacgcct aagttcgata
    16501 tcaaggaact ggcggggcgt gccggcgagt gaccattttc agccactatt gcaaaggtgg
    16561 cggcgtactc gttgcctccc tcgctttgtt ctcttccccg acgatacaag attaagtgaa
    16621 acttcatatt cacttcatgc cgtcttcatg ttcattttgg tacaattaat ctggtctaag
    16681 gaatggctgg tggcagcaca tatgttggga cggcagttgc actgcggacg cacggacttg
    16741 tgtataatca ccaggaggaa tcgtgattgc agcagcgcat aacttaagga gaaccaggat
    16801 gatgaatacg cttcgagatg ttcaccgaaa gatcagagcc atagcctccg ggcttcagag
    16861 ccgacgggtt agtctcctgg cctgtgggct tctcatcggc atcctcgtgg ccgcctgtag
    16921 cggtggttcg ccgtcaccca ctccatcggc caatacgccg tcatccgctc catcggccaa
    16981 tacgccggcg gcgagccagg ccactgctac gggtcaaagc cgtctgccgg gtccagagga
    17041 attcggcatg accacggagg agcttgtcac gagcgtcgag gctgtagaat cgctgatttc
    17101 caaatgcatg agtgatgccg ggttcgaata catcgccgtc gattacgaca ccgtccgcaa
    17161 aggcatggtc gccgacaaat cgcttcccgg agtcagcgac gacgactaca tcgcccagta
    17221 cgggtatggc atttcgacgc tgtataccgg tctgcccccg caactggctg atgtggccac
    17281 cccggcccaa ataggcctgg gtgaacagaa cgttcgaatc ttcaacagcc tgtcgcctgc
    17341 cgatcaggtg gcctacaacc ggacgctctt cggcgagaat atcgacgcga ctttagcggt
    17401 ttcgctcgag gccgaggact tctcgcgcac cggcggatgc acccgcgccg ccatcgagca
    17461 ggttttcgct cgggaacagt taagcgcgac ctatctcaat ccattggatg cccttatcga
    17521 gcaggatccg cgcatgatcg ccgcccttga cgaatttgcc agttgcgttc gcgaggaggg
    17581 tttcaactac agtcatccga atgagatcga gcctgatata aacaaccggc tcgatgccat
    17641 cacaaaaggc gctcctctcg aggcgttgtc ggctgaggcc caggccgccc tcgcagaact
    17701 ccaaggctat gagcgagcgg ttgccgcact tgccacttcg tgcgaggagg acatcgtcga
    17761 gccggttcag gctcagattg agagagaatt gtacggtggc caacagccat gactgaaacg
    17821 ggccgcgcga gtaaaggccg ccgacagaga ttcaccgccg ataagtcaag catcctgagc
    17881 gagtaagggt catcatgagc agaaaacgga acttaacact cttcattgtc cttgcgctgg
    17941 tggttcttgt ggcggcaggg agttggattg ccgggtcaaa tctcatgact ccggcagagg
    18001 cggccgcgcg aacggcgccg ccggtgccat cgcccattct ggtcccggtc gaggagcgcg
    18061 tgctgacgtc cgacatcgtg acgcgcggca cggcccggta cggacggcca caatccatct
    18121 cgattgttcc gtcccccttg aaacctggcg gagggatcat caccacgctg ccggtgcgga
    18181 atacccagtt gaatgagggt gatgtgatgc tcactgcttc ggggcgcccg gtattcattc
    18241 tccagggtga gatcccggcc taccgggacc ttgtccccgg cagttccggc gatgatgttc
    18301 gccagttgga gaatgcactc aagcggctgg ggtttgatcc cggccctgtt gacggatctt
    18361 tcgatgagga gaccagcaca gccgtggccg attggtatgc ctcggcggga tgggagccgt
    18421 ttgggccaac gcccgagcaa ctagccaata tccgtgccct cgaacaggaa ttggccgtag
    18481 ggataagcaa cagatcggcg gcctacgccg ccgccgccgc cgcccccctg gcagtggaag
    18541 ccgcacgcgc caacgccgat agcgccaatc aggctgctgc cgccgatgtg gctgacaaga
    18601 ccaggctccg ggattgggca accgatgaag accgggcgaa ggccaatgta gatctcgaag
    18661 tggctcgggc cggtgcgaca gccactcgat tggcaggtga aatggccgtt caggccgccg
    18721 ttgacgcaca gcaggccgcc gaacgtgagg ccaagctggc cgccgattca gtcgttcggc
    18781 tcgctgctga cctcgagatt gctcgacgca aggcaggtgt gcaggtgccg gccgatgaga
    18841 tcgtctttat tcccgctgtt cctgtccgtg tcgagcagat caacgtcgtg gtgggtgaag
    18901 cgccgagtgg gcctgtgatg acggtgacga attatcaatt agcgatcgac tcatccctcg
    18961 ccctcgacga agcgccgctc gtcaagccag gcctgccggt cgccattgac gaaccggacc
    19021 tcgggatcaa ggccacgggt gtcgtgggca gggtagccga tacccccggc accttcgggg
    19081 tcgacgggtt ccacatctac ttagaggttc ttgtcgacga gacccccgtt gcgctcgagg
    19141 gcttctcgtt gcgcctcacg attccggtca aatcaaccgg ggggatggtc accgtcgttc
    19201 cggtcagcgc gctgtcgctc gccgccgacg ggacttcaag agtccaggta gagcgcaatg
    19261 gctcgcttga gttcatcgcg gtcgagccgg gactatcggc cgacggcttc gtcgaggtga
    19321 ccccggttga tggagcgctg gcgccgggcc agctcgtagt gatcgggttt gagcagcaat
    19381 gatgacgacg actcagccca acacgacgaa atacgtcgtc gaactccata aggtcagccg
    19441 gacatttgga agcgaacccg ctgtccacgc gctcgtggag gtcgatctat ggttagagca
    19501 tggcgagtgg ctggcgatta ccggcccatc tggcgccgga aagtcaaccc tgctacacat
    19561 catcggctgc ctggaccgcc cgaccagcgg cagctacgcc ctggatggca tagacaccgc
    19621 cacgttgagg gacaaagaac gcgccggact acggagccgg cgcatcgggt tcgtgttcca
    19681 atccttccat ttgctgcccc accggtctgt gctggagaac gtgatgctgg cagaggtgta
    19741 ccgtaaggaa tcccaccgcg gtcgacgaga ccgcgcgctg gcagcgatcg cgcgggtcgg
    19801 gcttagtcag cgcgccgatt tcttaccgac gaagctgtca ggcggtgaga agcagcgcgt
    19861 cgccatcgcc cgcgccctcg tcggttctcc cagcctgctg ttgtgtgacg aaccgaccgg
    19921 aaatctggat tcgaaatcga gcgccgacct cctcgacctc tttgagtcct taaaccggca
    19981 aggtctcacg ctggtcgtgg tgacccacga cgagaaggtc gccaatcggg cgagccgccg
    20041 cgtgcacatc atcgatggct cactgacgga cgtcacgaat ggagaaaaca acaggaagct
    20101 gtcgccctcc ccaagcacaa gcgtgaagtc gagcgccgtt tcgcggtcgg gtatcacgat
    20161 ccgtgacctc ctcgaggaag cgattgcggg gatgttcgcc cgaccgtggc gtatggtgct
    20221 gaccatgttg ggcatcgtca ttgggctgac cgccctcgtg gcgacgctcg gcctgtcagc
    20281 caccgccaac aaccgcatca tcagtcaatt cgatgagctg gccgcgaccg ggatcgtggt
    20341 caccgcgaga cccggccccg caggtgtcga tcccaaggct ctgccctggg acgcgccggc
    20401 ccgtcttggc cgattgaatg gcgtggtcgc cgccggaacg ctgagtgagg tgaatatcgg
    20461 agacacgctg gttagctcgt cacccgtgaa agaccctcgc agccagactg ctttcaagct
    20521 ctcggtcaag gctgcctcac ctgacttgtt tacagccgtc agagccgatc tggagaccgg
    20581 acgttttcca gatctggggc attcggatcg ggtggaccgg gtcgccattc tcggcccggg
    20641 ggccgcactc cggctcggta tcgtgagcct cgagcagctc ccggccatcg ccatcggcga
    20701 taaactctac ctggtcattg gtatcctgcg cggcgtcgct cgccagcccg atctccttgg
    20761 ctcagtgatc atccctgaag gcacggcccg ccacgatttc ggcctggccg gccctggctc
    20821 ggtgatcgtc gagacccgca ttggcgcggc atctctcatc gtccggcaaa cacccctggc
    20881 gctgcggcct gacaacccac gcgtgattag agttgagctt cccccagaac cccagcgtgt
    20941 gcgcgaggcg gtgcagggtg acctgaatgt cacgttcctc ttgctgggcg gcctgtccct
    21001 gatcgtaggt gcgattggca tcgccaacat tatgctcgtg tccgtgatgg agcgcatcgg
    21061 cgagatcggc ctgcgtcggg ccatcggagc cactcgcggg cacatcgccg cgcagttctt
    21121 actcgagagc gcctctctgg gcgctctcgg aggcatcctc ggcgccagcc tcggagttct
    21181 cgtcgtggtg gctgtctccg ccttccaggc ctgggctccg gtgctagacc cagcggcgcc
    21241 cgtcctcgcc ccactggtcg gtggcgggat cggcttggtg tcgggcacct atccggcttt
    21301 gcgcgctgca cgcatggaac cggttgaggc gtttcgaaat tgaacctgac cgagcctgca
    21361 acatcccagg tagagagact cgcagacaag cgctacgtca cgctgatctt gcgcctgcgg
    21421 atcgaccggc gggagcgcct ggttcatggc gaggttgtgg atacggaggg caagtcgcaa
    21481 ggccggtttg ccgggtggcg agaaatgacc cgcacggtgc gtaattacct gatgagccat
    21541 tagccactcg aattttgtgc tattatcttt gacttgcggt acttgactca ttataatctt
    21601 tgcatccatc agtcagcagg atgtcttgaa tgcgcatctt gatcgtcgag gaccagccca
    21661 acgtctcaaa ctacgtcaaa cgggcattag aagaacaagg atacgcggtc gatttagcgc
    21721 gcaccgggcg ggaagccttg gattgggccg aagtcgtcga atatgacctg attgtgctgg
    21781 acattatgct gccggagatg gatggcatca cggtgtgccg tcgcttgcgg agtcaaggtc
    21841 atccggctgc gatcctgatg ttgactgccc gtgataccgt cgacgaccgc gtgactgggc
    21901 tggatgccgg cgccgatgat tatctggtca aaccgtttag cttgaaagag ctgctggctc
    21961 gactccgggc gctggctcgt cgtcgcacgg ccaagacttc aaccctgcaa gtcgcaaacc
    22021 tcagcctgga tacacgcacc cgcgtggtga tgcgcagcaa cacattcatc aaactgacgg
    22081 ccaaagaata cgccatactg gaatgtttga tgcgcgaacc cggccgggtt ttaacccgca
    22141 ccgagattgc cgagtccgtt tggaattatg atgcctacaa ccaatccaac gtggtggacg
    22201 tctacattcg caacttgcgg cgcaaaattg acgacgcttt cgacgtgaaa ctcatccaca
    22261 cggtgcgggg agctggttat tgcctgtcgg accaggatag cgatgcatga aacgcttgaa
    22321 aacgcttcgg gtgcggtttg ccctatggac ggcggggtta ctgtttgccg ctttaacgct
    22381 gtttggtctc tttgtgtacg cgaatatgtc tcgcagcttg gccgcagcag ttgacgagaa
    22441 gttgcgctta gttgtggttc aactggtagc agaagttgat gtggagggag gtgagccggt
    22501 catcaatgaa aacccaatcg aggatccgga atatgcccaa ctccgtgaac agggcttcag
    22561 tatgcggtta ctggacctga ccggacaggt ggtgcaggaa tttggcccgt atagagattt
    22621 gccgcaacct caattaagct ttattgtccc cgagcagccc ggcgagttca ctacccttat
    22681 cgacgccggc agtcaagacc cggttcgcgt ctataccact ccaattgttg ttgaggaaga
    22741 gttagtaggc acactgcaag ttgcccaaaa tctaaaggaa gtgaggcgca ccctcgattt
    22801 gttgctcata accctgctct taggcggacc gctgatcgtg attgtggccg gcagcggcgg
    22861 gtattttttg gcagcgcggg ccttagcccc gatagataaa ataacccgta tggcccgcta
    22921 catctctgcc gaagagttgt cggcccgcct gaacctgccc gagaccgatg atgaggtggg
    22981 gcggctggcc gccacttttg actcgatgtt ggcccggctg gacgatgcct tccggcggga
    23041 acgccagttc accgccgacg cgtcccatga attacggacc cctctgtccg ccatacacac
    23101 gattatcggc agcacgttag cgcggcggcg cgccccggca gagtatgaac aggccatgat
    23161 tgacctgggc caggaagccg agcgcatgag aacattgacc gaaggactcc tcgatctggc
    23221 gcgtcttgat gcgacccggc agcccgcgaa attcgagcgc gtcgatttgt caaacctgct
    23281 gaaagacgtt gttgactctc tccgtccact ggcagaagat cagggactga agctcattga
    23341 ccatgtgccg gataatggtc taaccttaat gggagatagt gatggactga tccggttatt
    23401 tgtgaacctg ttggacaatg ccatcaaata cactgaacaa ggttccatca ccatttcagc
    23461 ccatccgaca agtgaacaat tgctggaagt caccatccgt gataccggcg ttggcatgac
    23521 gcctgagcat ctgactcgca tatttgaccg cttttatcgt gtggatgcgt cccgtagtaa
    23581 ggatggcatc ggtttgggtt tggccattgt caaaaatgca gcccgcgctc acggcggaaa
    23641 agtatctgta gaaagcaaat ttggagaagg aacaacattt atcgtgcaat taccaacaaa
    23701 gtaatggtgt tgaggtgggc agtagctaac aacaggcccg gcatctgacc gatgccgggc
    23761 ctgtgatgat taaatttcag tactgaatag ggggactgtc ccccggaggg tatgctactc
    23821 cgccggtgaa gtgtgagcgt acacgtcctc gctgatctcg taggcgtgct tctccagttc
    23881 cagagcctca gcgcggacct ccgccagggc ttccgggtca agctgcacga gctcgaccag
    23941 ctctaggtcc tcttgcgttg catcgtctcc tctccagccg actcgaacct cgcccacgcc
    24001 gcgcgcgtag tacttgagct ggaaggcgcc cagctcgtcc aggctggact ctgcgatcac
    24061 cagaacgtcc tcgtagcagt cgacagggac acaggtctcc tggcccatct gatccacctg
    24121 cgcaaagtcc gtccactcga ccgcgggccc ccacccctga aagtaactgg gcatccccgg
    24181 ctgcggctct gcccacatct tgattcccgc ccgcgcgtct tcgaggccgg cgatccaggt
    24241 cggcgccttg acgaattcgc cgccctcgta ttcctccggg tactcaccca ggtaccagac
    24301 gttgccgtcg ttgtcctggg cgtaaaaggc gatctccttc tccaccacct cgccatcgct
    24361 gtagtccaca acccaggcga cgaccgtgcg cacaccctcg atctccttag tcaggtcggt
    24421 gacggtgaac tcaatgcgat gagcaaaggt ctcaccatct tcg
//
LOCUS       DBNHXB010000361         3895 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_771064, whole genome shotgun sequence.
ACCESSION   DBNHXB010000361 DBNHXB010000000
VERSION     DBNHXB010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_771064"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..374
                     /locus_tag="AC162G_14820"
     CDS             120..374
                     /locus_tag="AC162G_14820"
                     /inference="COORDINATES: protein motif:HMM:NF014059.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin AF2212-like protein"
                     /protein_id="HIR5077126.1"
                     /translation="MESVIAVYEKGVLRPTTPLALPEHARVRVHIVEEVIEAEVDHPL
                     LALANLGESAEVDISERAEDILAEEVRPRAGWSASDADDR"
     gene            complement(477..2753)
                     /locus_tag="AC162G_14825"
     CDS             complement(477..2753)
                     /locus_tag="AC162G_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR5077127.1"
                     /translation="MGIIATLLTWAVSGGTAHSFDDYRGWLEEAGFHCITQHSEKWLS
                     AEKPPDTGYWSLVTGHWSLVTDHWILHLIIGIDIGGTFTDFTAIDDAGRIRIQKRLST
                     PSDPARAVLDGLRDLDAPPGAVIVHGSTIATNALLERKGARTALVTTRGFADVIEIGR
                     QNRPKLYALEPLKPEPLVPRDLRFELDERIAADGSIVRSLDPAEVAALAGRLQKTSVE
                     SIAVCFLFSFLHPDHERLVQRTISNYQSPQLPGAGGERQSHPFISLSCEVLPEYREYE
                     RASTTTINAYVAPLIARYLGRLEAALGGRRLRIMQSNGGVISAATAASLAARTVLSGP
                     AGGIVGAFDAAKRAGFSRAITFDMGGTSTDVALCDGSLPTTTEGEIAGLPMRLPILDI
                     HTVGAGGGSIARVDAGGALIVGPESAGAEPGPVCYGRGGTQLTVTDANLVLGRLDADR
                     FLGGQMKLDMGAAQHAIAELAHASRITHHASLAWGVVRVANAAMERAMRTISVERGFD
                     PRPFTLVAFGGAGPLHACELADSLGIARVLIPPSPGVLSALGMATADTVKDYSRAVLR
                     KADAVEDSELDSLLAEMESRGREELLAEGVTPQDIVVQRALDLRYVGQSYEITVEEKP
                     RFSLPRSASGAFGKRGFLHAFHQLHAARYSHSHPGRPVEIVTARVKAIGLARPIEVAP
                     ESPAGEDATLAIVGAKPVWFEKGLVETTLYERERLRAGHRVGGPAVVFQLDTTTVIPP
                     GWSARVDQWRNLIVMRDE"
     gene            complement(2741..3256)
                     /locus_tag="AC162G_14830"
     CDS             complement(2741..3256)
                     /locus_tag="AC162G_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077128.1"
                     /translation="MVRKRLTTAFIIICMVLFIVVFVSLALSSTAQLEEFIAQSVVND
                     QWEQATYGYLSPEGSIVAHVLNLWSYEGPRTPLRAWRVEQGRLTEKKPEVFLEATDND
                     RTLWPPFTFYYDIEYMTPLVAKVEVATLYNRGIILDSRGGYAQTWTAICILGRWTVIS
                     KQIDSFWDWVS"
     gene            complement(3368..3616)
                     /locus_tag="AC162G_14835"
     CDS             complement(3368..3616)
                     /locus_tag="AC162G_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017326260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR5077129.1"
                     /translation="MKQVPLSEVKDDLSKYLRLAEKEEVVITRHGKPAGLLIGFKSED
                     DWFDYRLEHDPAFLERIEQARKSLRSGRGIPLEDIAER"
     gene            complement(3613..3894)
                     /locus_tag="AC162G_14840"
     CDS             complement(3613..3894)
                     /locus_tag="AC162G_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007353517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
                     /protein_id="HIR5077130.1"
                     /translation="MILSPEAVEDLRGLKADVRATVRDAIEIHLRHTPTKTSKSRIKR
                     LRGLARPQFRLRVDDVRIYYDVTDDTVEILAIIPKSEADAWLARVGESQ"
BASE COUNT          752 a         1160 c         1228 g          755 t
ORIGIN      
        1 gcgcgcagaa tcattgagca tgagtaactg gggcttacgc ccaagaacat gtggggatag
       61 gcctcgcgct catctggctc tatggtttcg ggtataatcg ttgtcaggag gtgaaacaca
      121 tggaatccgt gatcgccgta tatgaaaagg gtgttctgcg cccgacgacg ccgctggcgc
      181 tgcccgaaca tgcgcgcgtg cgagtgcata ttgtcgaaga ggtcatcgag gccgaggtcg
      241 atcacccctt gctggcgctg gcgaatctcg gcgaatccgc agaggtagat atatcggagc
      301 gagctgaaga catccttgcc gaggaagtcc gtccgcgagc gggctggagt gcgtctgatg
      361 ccgacgatcg ttgacactgg cttccttttc gcattggcga atgcgcgcga tgctcatcac
      421 ccccaaacac tgcgctgcgt tcgagttatt gccgtagtgc taaagacgat cacgtatcac
      481 tcgtcacgca tcacgatcaa attcctccac tgatcgacgc gcgccgacca gcctggcggt
      541 atgaccgtcg tcgtgtcgag ctgaaaaacg acggccggac cgccaacccg atggccggcg
      601 cgcaggcgct cacgttcgta caacgtcgtc tcgactagcc ccttctcgaa ccacaccggt
      661 ttagctccta cgatggccaa cgtggcatct tcgccggccg gactctcggg agcgacttcg
      721 atcgggcggg cgaggccgat ggccttcacc cgcgccgtga cgatttcgac cggacggccc
      781 ggatggctgt ggctgtagcg ggcggcgtgt agctgatgaa aagcgtgcaa gaaaccgcgt
      841 ttcccgaagg cccctgaagc ggagcgaggg agtgagaaac ggggtttctc ttcgacggtg
      901 atctcgtaag actgcccgac gtatcgcaga tcgagtgcgc gttgaacgac gatatcctgc
      961 ggggtgaccc cttcggccag cagctcttcg cgcccccgcg attccatctc tgccaacagc
     1021 gagtcgagtt ccgaatcctc cacggcgtcc gcttttcgga gcaccgcgcg cgaatagtcc
     1081 ttcacggtgt ccgcagtcgc catgcccagc gccgacagca cgccgggcga aggcggtatc
     1141 agcacccgcg caatacccag cgagtcggcc agctcgcaag cgtggagcgg cccggcgccg
     1201 ccgaatgcga cgagcgtaaa tggacgcgga tcgaaaccgc gttcgacgct gatcgtgcgc
     1261 atggcgcgct ccatggcggc gttggctacc cggacgacac cccaggcgag tgatgcgtga
     1321 tgcgtgatgc gtgatgcatg cgcaagctcg gcgattgcgt gctgggcggc gcccatgtcc
     1381 agcttcatct ggccgccgag gaagcgatcg gcgtcgaggc ggcccagcac caggttggca
     1441 tccgtgacgg tgagctgcgt cccgccccgg ccgtaacaca ccggcccagg ctcggcgccg
     1501 gccgactcag gcccgacgat caacgcgccg cccgcgtcga cgcgcgcaat cgatccgccg
     1561 cctgcgccga cggtgtgaat gtcgaggata ggcaggcgca tgggcaggcc ggcgatttcg
     1621 ccttccgtcg tcgtgggcag gctgccatcg cagagcgcca cgtccgtgct cgtgcccccc
     1681 atgtcgaacg tgatggcgcg gctgaagccg gcgcgcttcg ccgcgtcgaa cgcgccgacg
     1741 atgccgcccg ccggcccgct cagcaccgtg cgcgccgcga gcgatgccgc ggtggcggcc
     1801 gagatgacgc cgccgttcga ctgcataatg cgcagccgcc gcccgccgag cgccgcttcg
     1861 agccggccca ggtagcgcgc tatcagtggc gcgacgtagg cgttgattgt ggtcgtcgaa
     1921 gcgcgctcgt attcgcggta ttcaggcagt acctcgcagg agagggagat gaagggatgt
     1981 gattgcctct cgccgccggc gcctggcagt tgaggagact ggtaattgga gattgtgcgc
     2041 tgcaccagac gctcgtgatc gggatggaga aacgagaaga gaaagcagac ggcgatagat
     2101 tcgacgctgg tcttctgcaa ccggccggcc agcgcagcga cttctgccgg atcgaggctg
     2161 cgcacgatgg agccatcggc ggcgatgcgc tcgtccagct cgaagcgcaa atcgcgcggg
     2221 acgagcggct cgggcttgag tggttcgagc gcgtacagct tgggccggtt ctggcggccg
     2281 atctcgatga cgtcagcgaa gccgcgcgtc gtgacgagag ccgtgcgcgc acccttgcgc
     2341 tcgagcagcg cgttggtcgc gatcgtcgat ccgtggacga tgacggcgcc gggcggcgca
     2401 tccagatcgc gcaagccgtc gaggacggcg cgggccgggt cggagggcgt cgagagtcgc
     2461 ttttgaatgc ggatgcggcc ggcgtcgtcg atcgccgtga agtcggtaaa cgtgccgccg
     2521 atgtcgatgc cgatgatcaa gtgcagtatc cagtgatcag tgaccagtga ccagtgacca
     2581 gtaaccagtg accagtaacc ggtgtcgggc ggcttttcgg cggatagcca cttctcgctg
     2641 tgctgcgtga tgcagtggaa gccggcctct tcgagccagc ctcggtagtc gtcgaaggag
     2701 tgggccgtcc cgccgctgac ggcccaggtg agcagcgtgg ctatgatacc caatcccaga
     2761 atgagtcaat ctgcttactg atcacagtcc agcggcccaa gatacagatt gcagtccagg
     2821 tctgtgcata accaccacgg ctatccagga tgatgcccct attatagagt gtagccactt
     2881 ctactttggc cacaagcggt gtcatatatt cgatgtcata gtaaaacgta aatggcggcc
     2941 agagcgttcg gtcattgtca gtcgcctcaa gaaagacttc tggtttcttt tctgtgagac
     3001 gcccttgctc aactcgccaa gctctaaggg gggtcctcgg cccttcatac gaccagaggt
     3061 tcaatacatg cgcgacgatg gacccttctg gagaaagata gccgtatgtg gcttgctccc
     3121 attgatcgtt gacgacagat tgtgcaatga actcttccaa ttgtgcggta ctactgaggg
     3181 caaggcttac gaacacgact ataaacaaga ccatgcagat gattatgaag gcagttgtga
     3241 ggcgtttgcg aaccatttca gaatgggctg gctcccgagc gtgaatcgcc gcacgcgcac
     3301 tgccacgatc aagttcacgg cgcggggtgt ctatgacaag gacccgtgcg tttaggaggg
     3361 cgaatcatca tctctcggca atgtcttcga gtggtatacc ccgcccggat cgcaagcttt
     3421 tccgcgcctg ctcgatccgc tccaggaaag cggggtcatg ctctagccga taatcgaacc
     3481 aatcgtcctc ggatttgaag ccaatgagaa ggccagcagg tttaccatgc cgcgtgatga
     3541 cgacctcctc tttctcggcc aggcgtaagt acttggacaa gtcatctttg acttcagaca
     3601 gtggcacttg tttcattggg attctccgac tctggctaac caagcatctg cttctgattt
     3661 gggaatgatg gctagaatct caaccgtgtc gtcagttacg tcgtaataga tacgcacatc
     3721 gtcgacccgt aatcggaact gtggccgagc cagtcctcgc aggcgcttga tacgactctt
     3781 actggtctta gtcggtgtgt gccgcaaatg gatttcaatg gcgtcacgca ccgtagcgcg
     3841 cacatcggct ttcaggcctc gcaaatcctc gacggcttca ggcgagagga tgatg
//
LOCUS       DBNHXB010000362         1743 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2115744, whole genome shotgun sequence.
ACCESSION   DBNHXB010000362 DBNHXB010000000
VERSION     DBNHXB010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2115744"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..467)
                     /locus_tag="AC162G_14845"
                     /pseudo
     CDS             complement(84..467)
                     /locus_tag="AC162G_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011171640.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
     gene            complement(639..827)
                     /locus_tag="AC162G_14850"
                     /pseudo
     CDS             complement(639..827)
                     /locus_tag="AC162G_14850"
                     /inference="COORDINATES: protein motif:HMM:NF024842.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1235..>1743
                     /locus_tag="AC162G_14855"
     CDS             1235..>1743
                     /locus_tag="AC162G_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077131.1"
                     /translation="MAERQERQVLAPANTTSLRRGMVFAAIACSSAAALTFEIALTRV
                     FAVTQFYHFAFMTVSLALLGFGASGSALTAFPWLGRGEPRRWAVLALCQSAGTLGAYA
                     LANAIPFDSFSIAWDGRQVGYLAASYLALVAPFFFGGLVVGALLSEPGLDSHRVYAAN
                     LAGSGLGCL"
BASE COUNT          274 a          542 c          567 g          360 t
ORIGIN      
        1 tccttgccga accgtctgcc ccggaaccca acatcttcgt cggccacttc atccctcgtc
       61 cgcgcgaagc ggccctcatc ctttgctttc agtgggtttg gacccagttt gcgtatgcgc
      121 tccgtcatgg tctgggccgc gttcgcgaac gcgtgcgcct ggctgccgga catgaagaac
      181 atgtcgagcc gctcgccgcc gatcccgatt tcgtcgagaa tcgtcttcgc gcggcgcaag
      241 cgggcgcgcc cgcgctcgtt tccgcgcacg tgatggcagt tgccgatcgg gcaggccacc
      301 acgtacacgc cgtccgcgcc ttgctcgaag gccaccagca gatagcgcac gtcggtcttg
      361 ccgctgcacg gcatcctaat gaacttgacg ttggccgggt actgaatctg cagcgcgccg
      421 gcctgtccgg ccacgtcgag gaggtgcgtg ttggtcagag tgtggatgtt cggatgctgg
      481 ttgtgccgga tgtaagcggg cgagatcgag gggcgggtgc agccatagcc gtggtcggga
      541 gtgccccggc acagcacgca gtcgtgctgc gggaacatgt agccgagctg cgcgacgcgc
      601 ccgccgaggc agggcgtgtg ctcgacaagg aaaaccttca atccggcgtc ggcaaggtcc
      661 agcgcagcgc gcataccacc aatgccgccg ccaatgacca taacggcagc aacgggtgga
      721 ttgaaagccc gactcccgtg gaagtcggcc attgcgcctc ccttggtatc gtattgccca
      781 ccaccggcga gcataccgtg acctccccca tcccctggtg gtggcatttt tcataataag
      841 tatcttccgt gattgatcaa atctatagtg attagtctca cgtgatacgg gtgttaactc
      901 tcacgattcg gattgggctg gcagaggggc agatcagcgc atgttatgta atgtccggga
      961 ggcaagacgt ctcgcgccga ggaggaacgg cgaggcgtgt ctactctggc ccgccgcgct
     1021 cgccggccgg acgagagatt cctcactgcg ttcggaatga cacaccacaa gtggccgagc
     1081 aggtgcggga aaggcggctc atgcctgcgc ggtgcttgac aagctcccga tctggcagta
     1141 gcctgacact tgaggctgtg acctttgtag tttgaagttt ggagtttggg gtttcagctc
     1201 catatggcag tagcctgaca atcgaggctg cgacgtggct gagagacagg aacggcaggt
     1261 gcttgcccct gccaacacga cttctttgcg ccggggtatg gtcttcgccg cgatcgcgtg
     1321 ctcctcggcc gcggcgctga ccttcgagat cgcgctgacg cgcgtcttcg ccgtcaccca
     1381 gttctaccac ttcgcgttca tgacggtcag cctggccctg ctcggcttcg gcgcgagcgg
     1441 ctcggcgctg actgccttcc cctggctggg gcgcggcgag ccgcgtcgat gggcggtgct
     1501 ggcgctttgc cagtcggccg gcacgctcgg cgcctacgcg ctggcaaatg cgatcccgtt
     1561 cgactcgttc agtattgcgt gggacgggcg gcaggtcggt tacctggcgg catcttacct
     1621 ggcgctggtc gcgccgttct tcttcggagg tctggtcgtc ggcgcgctgt tatcggagcc
     1681 gggactcgat tcccaccgcg tctatgcggc gaacctggcc ggatcgggcc ttggctgcct
     1741 gtt
//
LOCUS       DBNHXB010000363         4846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3290157, whole genome shotgun sequence.
ACCESSION   DBNHXB010000363 DBNHXB010000000
VERSION     DBNHXB010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3290157"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1076)
                     /locus_tag="AC162G_14860"
     CDS             complement(198..1076)
                     /locus_tag="AC162G_14860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5077132.1"
                     /translation="MILVTGAAGKTGRSIIRVLAARGQHVRALARRETQADALSQIGA
                     HEIVAGDLLDEAALARAARGVRAVYHICPNVHPQEVAIGKSIIAAARGAGVRLFAYHS
                     VLHPQTESMPHHWSKLRVEEMLFESGLLYTILQPAPYMQNVLAEKEAIMTQGVYRVPY
                     SVQAPFSLVDLEDVAEAAAIVLTEPVHAGATYELCGPEVLTPSDIAAVWSAKLGRSVQ
                     AQPIAVEDWARRAKAAGLSEYAVDTLTRMFGYYDRYGLWGNSRVLSDLLGRPPARFEA
                     LVERTIHDPKIDETGI"
     gene            1205..3055
                     /gene="ade"
                     /locus_tag="AC162G_14865"
     CDS             1205..3055
                     /gene="ade"
                     /locus_tag="AC162G_14865"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HIR5077133.1"
                     /translation="MTVRFELNPILPGPIGLDDISQVPLLEQNGEQTESLAQAPALDA
                     IIAGMELSHFIKIARGDAPADLLLQGGKVVNVFTGEVHDADVAIADGRIVGLGRYAAE
                     ETIDVSGKVLAPGFIDAHVHIESSLVPPPEFARAVVPRGTTTVIIDPHEIGNVLGLDG
                     IRFMFDAAKYNPLSVYVMMPSCVPATNMETAGAVLHWYDIAPYQNDPWVLGLAEMMNF
                     PGVVQGAEDVLDKLRAFEKRVRDGHAPGLSGAALNAYVAAGIGSDHECTTLAEAREKL
                     RLGMHVFIREATNAHNLDTLLPLVTPENARRICWCTDDRQPADLLDQGHIDFIIRRAI
                     ALGLDPITAIRIGTLNPAEYFGLGDRGAIAPGRRADLLVLDDLRAVRAPMVFRGGRLV
                     ARDGELLPYERPVRPSFLRSSMNVDWDGVNFKVEATGRKAHVIGVVPDQIITEHLVED
                     VTIENGEVVQDVGLDLLKIAVIERHTHSGNHAVGFVKGIGLRKGAIASSVAHDHHNIV
                     VIGADDLSMMTAARAVGDMHGGMSVANNADVVVRLPLPIAGLMSDQPIERVRTQMDDL
                     LRAAHDMGSTLHDPFMAMSFLALAVIPELKITDQGLIDVVKFQKIPLFVE"
     gene            3589..4542
                     /gene="pyrB"
                     /locus_tag="AC162G_14870"
     CDS             3589..4542
                     /gene="pyrB"
                     /locus_tag="AC162G_14870"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR5077134.1"
                     /translation="MRSFLGRDILSLKDFEREEFFRVFQVARDLEPIARDRRNSELLK
                     HKTLVAAFYQPSTRTRLAHEAAMHRLGGHVTGFADPKMTRAGDFYQESIKDTVKMLEF
                     YGDVIVMRHFQQGAPHEAARWASIPVINGGDGWGEHPTQILTDLYTVLQEKGRIDGLK
                     WLAVGDMRMRTMHSLGYGLTQFDCPITFVAPPDMSLTEEFKAELRQYSLNFREAEHVE
                     QAIHDADVILVEPVVQPDYTKSRDERAGEVSLTPANYKITRELLATKARSDAILLHSL
                     PRMDEVPADVDITRWSRYWQEAFYGVVMRMALLALVLGAVE"
BASE COUNT          906 a         1502 c         1550 g          888 t
ORIGIN      
        1 gggaaacagc ctgatcaggt acagggccgc gatgagcagc agcaccggcg tgaacaggcc
       61 ccggctgcgg acgaagacga cgatcaggat gatcatgagc accagatcga cccagccaat
      121 cgtgaacgta tattgcgagg ggattgcctg ttgaagcagc atgtctagcc tcctgtggtc
      181 gataagctga gcacagatta tatacccgtc tcgtcaatct taggatcgtg gatcgttcgc
      241 tcgacaaggg cttcgaagcg agccggtggg cggccaagca agtcgctcaa cacgcgggaa
      301 ttgccccata gcccatatcg atcgtagtaa ccaaacatcc gcgtcagggt atcgacagcg
      361 tattcgctca ggccggcggc cttcgcgcgc cgcgcccagt cctcgacggc gattggttgt
      421 gcctgaaccg aacgccccaa cttcgcgctc cacacggcgg caatgtcgga aggcgtcagc
      481 acttccggcc cgcatagctc gtacgtcgca cctgcatgca caggctcggt cagcacgatc
      541 gcagcggcct cggcgacatc ctccagatcg accaggctga atggggcttg cacggaatag
      601 gggacgcgat acacgccttg tgtcatgatc gcttccttct cggccagcac gttctgcata
      661 tagggcgccg gttgcaagat ggtataaagc aagccggact cgaacagcat ctcttcgacg
      721 cgcagcttgc tccagtggtg cggcatggat tcggtctgcg ggtgcagcac cgaatgatag
      781 gcgaaaagtc ttacccctgc gccgcgcgct gccgcgatga tcgatttgcc gatcgcgact
      841 tcctgcgggt ggacgttcgg gcagatgtgg taaacggcgc ggacgcctcg cgcggctcgc
      901 gccagggcag cctcgtcgag cagatcgccc gcgacgattt cgtgcgcccc gatctggctg
      961 agcgcatcgg cctgcgtctc acgccgggcc agcgcgcgga cgtgttgtcc ccgcgccgcc
     1021 agcacccgaa tgattgagcg tcccgtcttt ccggcagcgc cggtcaccag aatcatcgcg
     1081 tctcctatca tcctgaaaga tcagtctata atagacccat ctttcacacc tcaccagtca
     1141 agaacctgtt tcggacccgc ttgttgctct ggctggactg ccaagtccaa ccgcaaacgc
     1201 ggacttgaca gtccgcttcg agctgaaccc gatcctgcca ggcccgatcg ggctggacga
     1261 tatttcgcag gttccacttc tggagcagaa cggcgagcag acggaatcac ttgcccaagc
     1321 acctgcactc gatgctataa tcgccggcat ggaactctcc cacttcatca aaattgcacg
     1381 aggcgacgcg cctgccgacc tgctcctcca aggcggcaag gtcgtcaacg tcttcaccgg
     1441 cgaggtgcac gacgccgacg tggcaatcgc cgacggccgc atcgtggggt tgggccggta
     1501 tgcggcggaa gagacgatcg acgtgtcggg caaggtcctc gcgcccggct tcatcgacgc
     1561 gcacgtgcac atcgagtcga gcctggtgcc gccgccggaa ttcgcacgcg ccgtcgtgcc
     1621 gcgcggcacg acgaccgtga tcatcgaccc gcacgagatc ggcaacgtcc tggggctcga
     1681 cggcatccgc ttcatgttcg acgccgccaa gtacaacccg ctcagcgtct acgtcatgat
     1741 gccctcgtgc gtgccggcga cgaacatgga gaccgccggc gcggtgctgc actggtacga
     1801 catcgcgccc taccagaacg atccatgggt gctcggcctg gccgagatga tgaacttccc
     1861 cggcgtggtg cagggcgccg aggacgtcct cgacaagctg cgcgcgttcg agaagcgcgt
     1921 gcgcgacggc cacgcgcccg gcctgagcgg ggccgcgctg aatgcgtacg tcgccgctgg
     1981 catcggctcg gatcacgaat gcacgacgct cgcggaggcg cgcgagaagc ttcgcctggg
     2041 catgcacgtc ttcatccgcg aggcgaccaa cgcgcacaac ctcgacacgc tcctgccgct
     2101 cgtcacgccg gagaacgccc gccgcatctg ctggtgtacc gacgatcgcc agccggccga
     2161 ccttctcgac cagggccaca tcgacttcat cattcgccgc gcgatcgcgc tcggcctcga
     2221 tccgatcacc gccattcgca tcggcacgct caaccccgcc gagtacttcg gcttgggcga
     2281 tcgcggcgcg atcgcgccgg gccgccgcgc cgacctgctc gtgcttgacg acctgcgggc
     2341 ggtgcgggca cccatggtct tccggggcgg caggctggtt gcccgtgacg gcgagttgct
     2401 gccgtacgag cgccccgtgc gcccctcctt cctccgctcg tcgatgaacg tcgattggga
     2461 cggggtgaac ttcaaggttg aggcgacggg tcgcaaggcg cacgtcatcg gcgtggttcc
     2521 cgatcagatc atcaccgagc acctggtcga ggacgtgacg atcgagaacg gcgaggtcgt
     2581 gcaggacgtg ggcctcgacc tgctcaagat cgccgtcatc gagcgccata cgcacagcgg
     2641 caatcatgcc gtcgggttcg tcaagggcat cggcctgcgt aagggcgcga ttgcgtcgtc
     2701 ggtcgcgcac gatcaccaca acatcgtcgt catcggcgcg gatgacctgt cgatgatgac
     2761 cgccgcgcgc gccgtcggcg acatgcacgg cgggatgagc gtcgcgaaca acgccgacgt
     2821 cgtcgtgcgt ttgccgctgc cgatcgccgg gctgatgtcg gatcaaccca ttgagcgcgt
     2881 gcggacgcag atggacgacc tgctgcgcgc tgcccacgat atgggctcga cgctgcacga
     2941 tccgttcatg gccatgtcgt tcctggcgct ggcggtgatc cccgagttga agatcaccga
     3001 ccaggggctg atcgacgtgg tgaaattcca gaagattccg ctgtttgtgg aatgaatatg
     3061 caggcaagga tgtaacatag cggcttccgt ttcggtttac atcggagttg agtctttagg
     3121 cagttgccgg ctaaagcctc gaacagcttt ccgaactccg tggcgagatt ggggtcgctt
     3181 ggggtacaag ggccatgctc gcacatctcc ttacgtgcag cgattgccgc gaagtattcg
     3241 cgaacaggct gctgatggaa gtgatgggca acgtggcgcg gagtcgatgg ggcagagcgt
     3301 cgaggccgga tcgggtcgag ggcgccgccg aacggcgcaa ggcacaggtc gatacggctg
     3361 catgacacga ctgaggtttt gcttaagacg aacaattcga ttagaatcag gctgtcggcg
     3421 ctatctgcgg ggggagcgag cggcgtaaat ccgagtctgc cgtagacggg tgggccgtcg
     3481 ccggcgcgag ttcgcttgcg tcgaggtggc ggcctgtttt gtccgaagat gctcaagctc
     3541 cctgcgccag ccccggccaa tcatcacacc gatcaaggag atactggaat gagaagtttt
     3601 ctgggacgtg acatcctgtc actcaaggat ttcgagcgcg aagagttctt ccgcgtcttt
     3661 caggtcgcca gggatttgga accgatcgca cgcgacaggc gcaacagcga attgctcaag
     3721 cacaaaacgc tggtggcggc cttctaccag cccagcacgc ggacgcgatt ggcgcacgag
     3781 gcggctatgc accgtctggg cggccacgtg accggtttcg ccgatcccaa gatgacgcgc
     3841 gccggcgact tctatcagga atcgatcaag gacacggtca agatgctgga gttctacggc
     3901 gacgtgatcg tgatgcgcca ctttcagcag ggggcgccgc acgaagcggc ccgctgggcc
     3961 tcgatcccgg tgatcaacgg cggcgacggc tggggcgagc atcccaccca gattctgacc
     4021 gacctgtaca ccgtcctgca ggagaaaggc cgcattgacg gcttgaagtg gctggccgtc
     4081 ggcgatatgc ggatgcgcac gatgcactcg ctgggctacg ggctgaccca gttcgattgt
     4141 ccgatcacct ttgtcgctcc acccgacatg tctctgacag aagagttcaa ggccgagctg
     4201 cggcagtaca gcctgaactt cagagaggcc gagcacgtcg agcaggccat ccacgacgcc
     4261 gacgtcatcc tggtcgagcc ggtcgtccag cccgattaca ccaagtcgcg cgacgagcgc
     4321 gcgggcgagg tcagcctgac gcccgcgaat tacaagatca ctcgtgagct gctggcgacg
     4381 aaggccaggt cggatgccat cttgctgcat tcactgccgc gcatggacga ggtcccggcc
     4441 gacgtcgaca tcacgcgctg gtcccgttac tggcaggaag cgttctacgg agtcgtgatg
     4501 cgaatggcgc tgctggcgct ggtgctcggg gccgtcgagt agtcgggtag tcgggtggtc
     4561 gggtggtcgg gtggtcgggt ggtcgcattt ctgaaggaga gcaggctccc acgcctgcga
     4621 atctgcccgc tttcttggca gaatcgagct tctcgattag aatgatattt gtcgattatg
     4681 gtttcggcgg ttacaggagt atcctggatg acagtcaaag agtgggcggc ggcctatcgg
     4741 gcagccaatg aaatggagca gcaagagcgc gtcgccagat tgccttggga gccggtcgaa
     4801 gaaagtgtgc gttcctactt ctcgctgtgt cggatggtgc ttgctc
//
LOCUS       DBNHXB010000364         3735 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3961324, whole genome shotgun sequence.
ACCESSION   DBNHXB010000364 DBNHXB010000000
VERSION     DBNHXB010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3961324"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="AC162G_14875"
     CDS             <1..695
                     /locus_tag="AC162G_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR5077135.1"
                     /translation="KTRRHLTEFWMVEPEIAYCDLDQLLEIEEQFVSYIVQTCLRERA
                     VELKYLGRDTGALEKIEPPFPRLRYDEAIDLLHALREKETDGELRELLKIEWGDDFGS
                     PHETKLTQQYDKPVFVVGYPTGVKAFYMEPWPGRPDVCKSADLLAPEGYGEIIGGSER
                     MADPDLLIKRIQEHGLPMEPYKWYVELRRYGSVPHSGFGLGVERTVAWICGLEHIREV
                     SPFPRLLNRLYP"
     gene            complement(750..986)
                     /locus_tag="AC162G_14880"
     CDS             complement(750..986)
                     /locus_tag="AC162G_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077136.1"
                     /translation="MSRPQREPNRTYTVYGSGAWGGCSAIQARLMEHGVCSYTGWAPS
                     PPRWRFLFDTYLSPPEIHSLLADLWGKYDVKLEY"
     gene            complement(1176..2282)
                     /gene="tgt"
                     /locus_tag="AC162G_14885"
     CDS             complement(1176..2282)
                     /gene="tgt"
                     /locus_tag="AC162G_14885"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR5077137.1"
                     /translation="MNPAPFSFDLLAHDGRARAGVFHTPHGAIPTPVFAPVGTAATVK
                     AVPPRDLKELGAALILANTYHLFLRPGDELIARMGGLHAFMGWDGPILTDSGGFQVFS
                     LKEIRTIDDDGVTFKSHLDGSLHRFTPEKVIAVEEHLGADIIMALDECAPPLDRAYNE
                     QALRRTHAWAERCLRAKTRTDQALFGIVQGGVFDDLREQSARFLVDLDFPGYAIGGLA
                     VGETKDQMHHVLDVVDPLLPEGKPRYLMGVGTVEDFFETVARGVDMFDCVLPTRLARH
                     GAALTHGGRLNLRNSALAEDARPIEEGCACYACLHFTRAYIRHLMQANEILGLYLASI
                     HNLHFLLDTMRRIRESILTGAFTQLKREMRNQVF"
     gene            complement(2350..2700)
                     /locus_tag="AC162G_14890"
     CDS             complement(2350..2700)
                     /locus_tag="AC162G_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009761345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 domain-containing protein"
                     /protein_id="HIR5077138.1"
                     /translation="MTVSRLIRLEGLAVFVVSILLFGASNGTWWVYLLFFLAPDLSML
                     GYLRGPRTGAWVYNLVHTYVAPIVVIVIGYAMKSDPMLLAGIIWLGHIGADRLLGFGL
                     KYPTAFKDTHLQRV"
     gene            2746..>3735
                     /gene="alr"
                     /locus_tag="AC162G_14895"
     CDS             2746..>3735
                     /gene="alr"
                     /locus_tag="AC162G_14895"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR5077139.1"
                     /translation="MSVVKARSECYTHVVMLQAGATSPSPISNLPRPTWAEIDLDATE
                     YNVRRIRQIVGPRVEVMAVVKAEAYGHGAIEVARTALQSGATWLGVAALNEALPLRQA
                     GITAPILVLGWTPGWLAKQAIENDVACTVFDLDTAGVFHRTAHEAGRRARVHVKVNTG
                     MNRLGLPPEEAADAIQQMHALDGLSLEGIFTHFAKADKADPSYTLQQIDRFHHILREM
                     ERRGIRIPIRHAANSPGILRFPESHFDLVRLGIAMHGLDPSGDVPAPLDFRPAMALKT
                     AVAQVTVLPPGSPVSYGGEYVTRGTERIAVLPIGYADGFRRRPRTWGEVLVHGR"
BASE COUNT          730 a         1146 c         1203 g          656 t
ORIGIN      
        1 cgaagacgcg ccgccacctg accgagttct ggatggtcga gccggaaatc gcctactgcg
       61 atctcgatca actgctggag atcgaagagc agttcgtcag ctacatcgtg cagacgtgcc
      121 tgagagagcg cgcggtggag cttaagtatt tgggccgcga cacgggcgcg ctggagaaaa
      181 tcgagccgcc tttcccgcgc ctgcgctacg atgaggcgat cgatctgtta cacgcactgc
      241 gcgagaagga aacagacggg gaactgaggg aattactgaa gatcgagtgg ggcgacgact
      301 tcggatcgcc gcacgagacg aagctaaccc agcagtacga caagccggtg ttcgtcgtcg
      361 gctacccgac gggggtgaag gcgttctaca tggagccgtg gccgggtcgg cccgatgtgt
      421 gcaagagcgc cgacctgctc gcgcccgagg gctacggcga gatcatcggc ggttcggagc
      481 gcatggcgga tccggacttg ctgatcaagc gcattcaaga gcacggcctg ccgatggagc
      541 cgtacaagtg gtacgtggaa ttgcggcgct acggctcggt cccgcactcc ggtttcgggc
      601 tgggtgtcga gcggacagtc gcctggattt gcggcctgga gcacatccgt gaggtgtcgc
      661 cgtttccaag actcctgaac cggttgtacc cctagcaaag aatcctcccg caggcttcag
      721 agcctgcggg aggattgttc ctaatcgggt cagtactcaa gcttcacgtc gtactttccc
      781 cacagatcgg ccagtaacga gtgaatctct ggcggactaa gatacgtgtc gaacaggaag
      841 cgccagcgcg gcgggctggg cgcccatccg gtgtaagagc agacgccgtg ttccatcaac
      901 ctggcctgaa tggccgaaca cccgccccac gcgccgcttc catagacggt atatgtgcgg
      961 ttgggttcgc gctgcggccg gctcatcggt ctccacgccc acctaacagg ctcacaaagt
     1021 tgtcctgttc atcatagtct accttaggag tgaagtcaag ccgattcggt tacggtacga
     1081 ttacgctggc tcccaaacac ccccaacgcc gtcacaatcg tttgaatcta ccccacaggg
     1141 cgcgctgcac gatgagttgc ggagaaacca ggtttctaaa agacctggtt tctcatttcg
     1201 cgcttgagtt gcgtgaacgc gcctgtcaaa atcgactcgc ggatgcgacg catcgtgtcg
     1261 agcaggaaat gcaggttgtg gatcgaggcc aggtagaggc cgagtatctc gttggcctgc
     1321 atcaagtggc gaatgtaggc gcgtgtgaag tgaaggcagg cgtagcaggc gcatccctct
     1381 tcgatggggc gcgcgtcttc ggccaaggcg gagttgcgca ggttcagccg cccgccgtgc
     1441 gtgagcgccg cgccgtggcg cgccaggcgc gtcggcagca cacagtcgaa catgtccacg
     1501 ccgcgtgcga cggtctcgaa aaaatcctcg acggttccca cgcccatcag gtaacgcggc
     1561 ttgccctcgg gcaggagcgg gtcgaccacg tcgaggacgt gatgcatctg atccttcgtc
     1621 tcacccacag ccagcccgcc gatggcgtaa ccggggaaat cgagatcgac caggaaacgc
     1681 gccgactgct cgcgcaggtc gtcgaatacg ccgccttgca cgatcccgaa cagcgcctga
     1741 tcggtgcgcg tcttggcgcg caggcagcgc tcggcccagg cgtgcgtgcg gcgcagcgcc
     1801 tgttcgttgt acgcgcggtc gagcggcggc gcgcactcgt cgagcgccat gatgatgtcc
     1861 gcgccgagat gctcttcgac ggcgatcact ttctcgggcg tgaaacggtg caggctgccg
     1921 tcgaggtgcg acttgaacgt cacgccgtcg tcgtcgatcg tgcgaatctc cttgagcgag
     1981 aacacctgga agccgcccga gtcggtgagg attggcccgt cccagcccat gaatgcgtgc
     2041 aggcctccca tgcgcgcgat cagctcgtcg ccgggacgca ggaacaaatg gtaggtgttc
     2101 gccaggatca gcgccgcgcc cagctccttc aggtcgcgcg gggggacggc tttgaccgtc
     2161 gccgctgtgc cgaccggcgc gaagaccgga gtcgggatcg cgccgtgcgg ggtgtgaaag
     2221 acgcccgcgc gggcgcgtcc gtcgtgggcg aggagatcga aactgaaagg agcaggattc
     2281 attggatcgt caaatgtgaa gcgtaagacg tgaaagcgta aaacgtgaag cgtgatgggc
     2341 gtgatcgcgt tagactcttt gcaggtgcgt gtccttgaat gcggtcgggt atttgaggcc
     2401 gaagccgagc aatcgatccg cgccgatgtg gccgagccag atgatgcctg cgagcagcat
     2461 gggatcggat ttcattgcgt atccgatgac gataaccacg atgggcgcca cgtaagtgtg
     2521 caccaagttg tagacccatg cgcccgtgcg agggccgcgc aggtagccca acatcgagag
     2581 atcgggcgca aggaagaaca ggagatacac ccaccatgtt ccattcgatg cgccgaacaa
     2641 gaggatggat acgacgaata ccgccagccc ttccaggcga atgaggcgac tcacagtcac
     2701 ccgaatgctc ccacggctat tgtgccctgg attataagat tcactgtgag cgttgtcaaa
     2761 gcccgatccg aatgctatac tcacgttgtg atgctccagg caggcgcgac atctccatct
     2821 ccaatctcca atctccctcg ccccacctgg gccgaaatcg acctcgacgc gaccgaatac
     2881 aacgtcaggc gcatcaggca gatcgtcggc ccgcgcgtcg aagtcatggc cgtcgtcaaa
     2941 gccgaagcct acggtcacgg cgcgatcgag gtcgcgcgca cggcgctgca atcgggcgcg
     3001 acgtggctcg gcgtggccgc gctcaacgag gcgcttcccc ttcgacaggc cggaatcacg
     3061 gccccgatcc tcgtcttggg atggacgccg ggctggctgg cgaagcaggc gatcgagaac
     3121 gacgtcgcct gcacggtctt cgatctcgat acggcgggcg tgttccatcg aacggcgcac
     3181 gaagccggcc gcagagccag ggtgcacgtc aaggtcaaca ccggcatgaa ccgcctcggc
     3241 ctgccgccgg aagaagcggc ggacgcgatt cagcagatgc acgcgctgga cggcctgtcg
     3301 ctggaaggca tcttcaccca cttcgccaag gcagacaaag ccgatccatc ctatacgctc
     3361 cagcaaatcg accgtttcca ccacatcttg cgcgaaatgg aacggcgcgg tatccgaatc
     3421 ccgatccggc acgcggccaa ctcgcccggt atcttgcgct ttcccgagag ccattttgac
     3481 ttggtgcgcc tgggcatcgc catgcacggc ctggatccca gcggcgacgt gcctgcgccg
     3541 ctggacttcc gccccgcgat ggcgctgaag acggcggtgg cgcaggtcac cgtgctcccg
     3601 cccggatcgc ccgtttctta cgggggcgaa tacgtgacgc gaggcaccga gcgaatcgcc
     3661 gtgctgccga ttgggtacgc cgatgggttt cgtcgccggc cacgcacctg gggcgaggtg
     3721 ctcgtgcacg gccgg
//
LOCUS       DBNHXB010000365         7299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_439253, whole genome shotgun sequence.
ACCESSION   DBNHXB010000365 DBNHXB010000000
VERSION     DBNHXB010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_439253"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1261
                     /locus_tag="AC162G_14900"
     CDS             83..1261
                     /locus_tag="AC162G_14900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5077140.1"
                     /translation="MPSATSYLPFAICHPPMTLALVHDWLNQYGGAERVLEVLVEMYP
                     SAPLYTSIYWRDGMPPGYRAWDIRTSWLDRAPGIYQHHQPYLPLYPLAFRSFDLGGYD
                     VVISNKSGFCHGVPAGDALHVCYCLAPTRYVWDLDAYVEREALGRAAQVALKPLIAWL
                     RRWDYAAAQRVTHFVAISSEIQGRIRKYYNRDAVVIHPPADLHRYQPAASHDDFYLIV
                     SRLIPYKRIDVAIEAFNRLRRPLWIAGAGRDAERLQSMAGPTITFLGRVPDSDLPDLT
                     ARCKAFVFPGHEDFGIAPVEAQAAGRPVIAYGVGGALDSVIDGVSGVLFHEQSAGALM
                     DAVHRLDAIRFDPGAIRDNAARFDAQAFKLKLTAFVDKCVSEWTTNQRSSESATGRSR
                     "
     gene            1297..1977
                     /locus_tag="AC162G_14905"
     CDS             1297..1977
                     /locus_tag="AC162G_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0061097 - regulation of protein tyrosine
                     kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YveK family protein"
                     /protein_id="HIR5077141.1"
                     /translation="MELRQLVRILLRRWWLVVLPALVLGAVAIVTYDPPQSTFAATMR
                     FAVGYTPDPNSQSLYDRFYPAWLASEYIAGGLSDWARTGNFAEAVSEELAARDVDVPP
                     AAVAASIVSDHTRSIVALYFNGNDPALLQSIAESAARVLQTRNATVFPQNGPHGASVT
                     PLDNPAVGAAPPSLRARLELPIRIGLAMAAGLVLALLAHYLDPMIRDRSDVEALGWPI
                     IGEIPKSK"
     gene            2084..2731
                     /locus_tag="AC162G_14910"
     CDS             2084..2731
                     /locus_tag="AC162G_14910"
                     /inference="COORDINATES: protein motif:HMM:NF014732.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0061097 - regulation of protein tyrosine
                     kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YveK family protein"
                     /protein_id="HIR5077142.1"
                     /translation="MSEYLRILRKRWWIIALVTLATAAAALGFSAAQTPVYKSTIQLS
                     VWPDRIDNGAQLATKALLRGFAAHVNSLKFADRVIDDLQLDMFAEQLKEKVTIAPIEE
                     DFIIQIDVLDTDPALANNIAEKWALRVVEWRNQENQEQRKEDRVSAAIIQGATYGKFR
                     PQTTLNTIAGAIIGLTLGVLIVFVLEWIESGVLRTPEDVERAMGMSVLGAIPSTK"
     gene            2804..3475
                     /locus_tag="AC162G_14915"
     CDS             2804..3475
                     /locus_tag="AC162G_14915"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HIR5077143.1"
                     /translation="MSNKSPSLVTLSDPRSPMAEAYRTLRTNIEFSSLDQSLHTLLVT
                     SPAPSEGKSTTLANLAVTMAQAEKKTILVDADLRRPSLHTIFNVGNEAGLTTMFVDRE
                     AMQEPPLQDVGVANLWLLPSGPLPPNPAELLGSKRMENALAALKARADIVLLDAPPVI
                     AVTDAVVLGTRVDGVLLVVGAGQTKRDHARRARELLDKVKVRVIGVALTNVSPDSALG
                     GYYSG"
     gene            3561..4625
                     /locus_tag="AC162G_14920"
     CDS             3561..4625
                     /locus_tag="AC162G_14920"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HIR5077144.1"
                     /translation="MKGLILSGGKGTRLKPLTFTSAKQLIPVANKPVLFRVIEAIRDA
                     GVTEVGIVIGDTGPQIRDAVGDGSRWGVSIAYIPQDAPLGLAHAVKISRDFIGDDRFV
                     MFLGDNVIQGGISPLLGQFATSDYDAQIVLKRVADPQHYGVAELEDGRIKRLIEKPKE
                     PPSNLALVGIYMFNHHIFEAVNAIQPSWRNELEITDAIQYLVEKKYAVYPYVHEGWWI
                     DTGKMADMLTANSLVLQELTPRVEGTVDAMSQIEGQVTIEKGADIVNSRIRGPVIIGE
                     ETRIVDSFIGPFTSIYHHCLIQHSEIEHCIVLENTRIVDIPARIADSLIGRNVEVTRS
                     PIKPKAVKLTLGDYSKVGIL"
     gene            4824..5054
                     /locus_tag="AC162G_14925"
     CDS             4824..5054
                     /locus_tag="AC162G_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007889586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR5077145.1"
                     /translation="MSNGARLTGTVRWFSRVKGYGFIKPDDSEKDVFVHYSGIVGDGY
                     RNLDEGQRVEFIIEDTAKGPQAVQVVGVAGNA"
     gene            5374..5571
                     /locus_tag="AC162G_14930"
     CDS             5374..5571
                     /locus_tag="AC162G_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019424259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HIR5077146.1"
                     /translation="MRGTVRWFDISKGYGYIQPENGVEVFVHYTAIIDKGLKMLNPGQ
                     RVEFTVTDSYRGPLAADVVRV"
     gene            5716..>7299
                     /locus_tag="AC162G_14935"
     CDS             5716..>7299
                     /locus_tag="AC162G_14935"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR5077147.1"
                     /translation="MASPLRIVPLGGCGEIGKNMTAFEYGRNILLIDAGIMFPENDML
                     GIDFIIPDWQYLRDKKDLVRAIVVTHGHEDHTGALPYLLKDGFNAPIYATKLTRGLLE
                     VKLKQHKVFEEANLNTIAAGEAVTIGPFKMDFFRVAHSIPDGVGLGITTPAGLVVHTG
                     DFKFDHTPVDGKPTDFARLAEFGGRGVLALMSDSTNADQPGTTPSERVIDEAFDRVFR
                     EAQGRIIIGTFASLISRIQQAANAAMRYRRRMAIAGTSMAENVRMAQKLGYLTFPDDL
                     IVRLDEAARMRPRDIVIMATGTQGEPSSVLARVAAGEHSQIKVQRGDTVVMSSHPVPG
                     NEEMVHRTINRLFQRGAEVLYDPIEMVHVSGHASQEEQKLMLNLVRPEHFIPIHGELR
                     HLHAHARLARLVGVEPTNIHVVENGYPIEFDEGEARIGERVPGGYVYVDGSGVGDIGP
                     SVLREREMLGRDGFVMTIVHRDPENGRLLDTPDIVTRGFVYAREAQDLLEGATQAVIQ
                     IVESNGRASQEAIRDRVEKS"
BASE COUNT         1436 a         2291 c         2287 g         1285 t
ORIGIN      
        1 acagctcaaa gtgcacttga tgaattggaa gcccatgcca cagaagccac cgatacgcga
       61 taagcgatag gcgataagcg atatgccatc agccaccagc tatctgccat ttgccatctg
      121 ccacccaccg atgaccctcg ccctcgttca cgactggctc aatcaatatg gcggcgcgga
      181 gcgcgtgctg gaagtcctgg tcgagatgta cccctcggcc ccgctgtaca ccagcatcta
      241 ctggcgcgac gggatgccgc ccggctatcg ggcctgggac atccgcacgt cgtggctcga
      301 ccgcgccccg ggcatctacc agcatcatca gccttacctg ccgctgtatc cgctcgcgtt
      361 ccgatccttc gaccttggag gctacgacgt cgtgatcagc aacaagagtg gattttgcca
      421 cggcgttccg gcgggagacg cgctgcacgt ttgctactgc ctggcgccga cacgctacgt
      481 gtgggacctc gatgcctacg tcgagcgcga ggcgttggga cgcgccgcgc aagtcgcgct
      541 caagccgctc atcgcctggc tgcgccgctg ggattacgcc gccgcgcagc gcgtgacgca
      601 cttcgtcgcc atcagcagtg aaatccaggg acgcattcgg aagtactata atagggatgc
      661 cgtcgtcatt cacccgccgg ccgaccttca ccgctatcag cctgccgcgt cgcacgacga
      721 cttctatctc atcgtctcgc gcttgattcc gtataagcgc atcgacgtgg cgatcgaggc
      781 tttcaaccgg ctgcggcggc cgctgtggat cgcaggcgcg ggacgcgatg cggagcggct
      841 gcagtcgatg gccgggccga cgattacatt cttaggtcgt gtgcccgatt ccgatttgcc
      901 cgacctgacg gcgcgctgca aggcgttcgt ctttcccggc cacgaggatt tcggcatcgc
      961 gccggtggag gcgcaagcgg ccggccggcc agtgatcgcc tacggcgtgg gcggcgcgct
     1021 cgattcggtc atcgatgggg tgagcggcgt gttgttccac gagcagagcg ccggcgcgct
     1081 gatggacgca gtgcaccgcc tggacgctat ccgcttcgac ccgggcgcga tccgcgataa
     1141 cgcagcgcgc tttgacgccc aggcgttcaa gctgaagttg accgcgtttg tggacaagtg
     1201 tgtcagcgaa tggacaacga atcagcgaag cagcgaatca gcgaccggac gatctcgctg
     1261 actcgctgat tcgccacctg ctggtcaagg tacaacatgg agcttcgcca actcgtccgc
     1321 atcctgctgc gccgctggtg gctggtcgtg cttccggcgc tcgtgctggg cgctgtcgcc
     1381 atcgtcacct acgacccgcc gcaatcaacc ttcgccgcca ccatgcgctt cgccgtggga
     1441 tacacgcccg acccgaactc acagtcactg tacgatcgct tttacccggc ctggctggcg
     1501 agcgaataca tcgccggcgg gttgagcgac tgggccagga caggcaactt cgccgaagcc
     1561 gtgtcggaag agctggcggc gcgggatgtc gacgtaccgc ctgcggcagt cgccgcatcg
     1621 atcgtgtccg accacacacg cagcatcgtc gcactgtact tcaacgggaa cgacccggcg
     1681 ctgctgcaat cgatcgccga gtcggcggcg cgcgtgctgc agacgcgcaa cgcaaccgtc
     1741 ttcccgcaga acggcccgca cggcgcgtcg gtcacacccc tggataaccc ggccgtcgga
     1801 gcggcgccgc cctcgctccg cgcgcggctc gaactgccga tccgcatcgg gctggcgatg
     1861 gccgccggcc tggtgctcgc gctgctcgcg cactatctcg acccgatgat ccgcgaccgg
     1921 agcgacgtcg aggcgttggg ctggccgatc attggggaga ttccgaagag caagtaattg
     1981 gagattagag attagagatc ggcgattggc aatatgcgat atgcgataag cgatatgcga
     2041 agtatggtag aatcgccatg ccgaaggggg actcaagatg gaaatgagcg agtatttacg
     2101 catcctgcgc aagcggtggt ggatcatcgc gctggtcacg ctggcgactg ccgcggcggc
     2161 cttaggcttc agcgcggcgc agacgccggt ttataagagc acgatccaac tgtcggtgtg
     2221 gccagatcgg atcgacaacg gcgcacaact ggcgaccaag gccctgctcc ggggattcgc
     2281 cgcccacgtg aactctctca agttcgccga tcgggtgata gacgatctac aattggatat
     2341 gtttgccgag caactcaaag aaaaggtgac tattgcaccg atcgaagagg atttcatcat
     2401 ccagatcgac gtgcttgaca ccgatccggc attggccaac aacattgccg aaaagtgggc
     2461 gctccgggtc gtcgaatggc gcaaccaaga gaaccaggag cagcgtaaag aggaccgcgt
     2521 ctcggcggcc atcatccaag gggcaaccta cggcaaattc cgcccgcaga cgacgctgaa
     2581 cacgatcgcc ggcgcgatca tcggcctgac gctgggcgtg ttgatcgtat tcgttctgga
     2641 gtggatcgag tccggcgttt tgcgcacgcc ggaggacgtc gagcgcgcca tgggcatgag
     2701 cgtgctcggc gcgatccctt cgacaaaatg atacgtgatg cgtgacacgt gacgcgtgat
     2761 gcacatatca cgcgtcacgt gtcacgcaga aaagatcgaa cgcatgagca acaagagtcc
     2821 ctcattagtg acgctttccg atccacgctc tcccatggct gaagcgtatc gcacgctgcg
     2881 caccaatatc gagttttcca gcctcgacca gtcgctccac acgctgctcg tcacctcgcc
     2941 ggcgccgagc gaaggcaaat cgacgacact ggcgaacctg gccgtgacga tggcccaggc
     3001 cgagaagaag acaatcctgg tcgacgccga cctgcgccgc cccagcctgc atacgatctt
     3061 caacgtcggc aacgaggccg gtctgacgac gatgttcgtc gacagggaag ccatgcagga
     3121 gccgccactg caggacgtcg gcgtggcgaa cctgtggctg ctgccctccg gcccgctgcc
     3181 gcccaacccc gccgaactgc tcggctcgaa acggatggag aatgcgctcg ccgcgctcaa
     3241 ggcgcgcgcc gacatcgtgc tgctcgacgc gccgccggtg atcgccgtga ccgacgcggt
     3301 cgtgctgggc acgcgcgtcg acggcgtgct gctcgtcgtc ggcgccggac agaccaagcg
     3361 cgaccacgcc aggcgcgcca gggagctgct ggacaaagtg aaagtgcgcg tgatcggcgt
     3421 tgcgctcacc aacgtcagcc cggactcggc gctcggcgga tactattctg gctaaccgtc
     3481 tgcgccacat cggtttgtga cgaagctcga agccgaagtc ttctccgatt ccaccggcta
     3541 actccttcta aggatactcc atgaaaggcc tcattctcag cggcggcaag ggcacgcgtc
     3601 tgaaaccgct caccttcacc agcgccaagc agttaattcc ggtcgccaac aagccggtgc
     3661 tcttccgcgt catcgaagcc atccgcgacg ccggcgtcac cgaggtcggc attgtgatcg
     3721 gcgacaccgg gccgcagatt cgcgacgccg tcggcgacgg gagccgctgg ggcgtgagca
     3781 tcgcgtacat cccgcaagac gcgccgctcg gcctggcgca cgctgtgaag atcagccggg
     3841 acttcatcgg cgacgaccgc ttcgtgatgt tcctgggcga caacgtgatt cagggcggta
     3901 tcagcccgct gctgggccag ttcgctacca gcgactatga cgcgcagatc gtgctgaagc
     3961 gcgtcgccga cccgcagcac tacggcgtcg ccgagctgga agacgggcgc atcaagcgcc
     4021 tgatcgagaa acccaaagag ccgcccagca acctggcctt ggtcggcatc tacatgttca
     4081 atcatcatat cttcgaggcg gtcaacgcga tacagccgtc gtggcgcaac gagctggaga
     4141 tcaccgacgc gattcaatat ctggtcgaga agaagtatgc cgtttatcct tacgtccacg
     4201 agggctggtg gatcgatacc ggcaagatgg ctgacatgct gacggcgaac agcctggtgc
     4261 tgcaggaact gacgccgcgc gtcgagggga cagtcgatgc gatgtcgcag atcgaaggac
     4321 aggtgacgat cgagaaaggc gcggacatcg tcaacagccg catccgcggg ccggtgatca
     4381 tcggcgagga gacgcgcatt gttgattcgt tcatcgggcc gttcacgtcg atctaccacc
     4441 actgtctgat ccagcacagc gagatcgagc actgtatcgt gctggagaat acgcgcatcg
     4501 tcgacattcc ggcgcgcatc gcggacagcc tgatcggccg caacgtggag gtgacacgct
     4561 cgccgatcaa gccgaaggcg gtgaaattga cgctcggcga ctacagcaaa gtgggcattc
     4621 tgtagccaca tcgttttgac atcgcccgga acatgtggta agctagcgct actcatcccc
     4681 catatctggc tcacaacagg accaattcga tcgaacctct gagcgacgcc cgattcgctg
     4741 tagcagtttg cgttgtctag ggagcagcac agagccgagc gctctgatgt cgaacagcaa
     4801 cacaatgtaa ggagaactca tctatgtcca acggtgcgcg cttgaccgga acggttcgct
     4861 ggttcagccg ggtgaagggc tacggcttca tcaagcccga cgacagcgaa aaagacgttt
     4921 tcgtccatta ttcgggaatc gtgggcgacg gttatcgcaa ccttgacgag ggccagcgcg
     4981 tcgaattcat catcgaggat acggccaaag gcccgcaggc ggtgcaggtg gtcggcgttg
     5041 ccggcaacgc ttagccgtcc atctatcgct cacagtcggc gtgtggctat tcgcgctcgc
     5101 cggtgcggaa cacggcgctg tctggcgccg caacaagagg tttgtggctt cgcgacgggc
     5161 ctcggggcgc cttcgaggcc gcgtatggct gtgcgtgatt tgacaggtcg ttcgaggtgt
     5221 gatatatttt gatttgtatg gggcattgca gcatctggtt gtctgaatcg acgaaataag
     5281 ccgccagggc gaggtaggca ccggcggctt ttgtttgcgg cgcgcgcgaa gatcattctt
     5341 cgaactcatc agaggattca gatggacaaa cgcatgcgtg gaaccgtgcg ctggttcgac
     5401 atctcgaagg gttacggata tattcagccg gaaaatggcg tagaggtatt cgtgcactac
     5461 accgccatca tagacaaggg gctgaagatg ctgaaccccg gccagcgcgt ggagttcacc
     5521 gtcacggatt cctatcgcgg cccgctggcc gccgacgtgg tgcgcgtgta gctgcgccgc
     5581 actatggcga actgcatatt gaccggaatc gccccgccgg ttggataacc gtcggggcgg
     5641 tttgattatt agccttcacc cctaccccag cttcgccaca gggggagggg gtgaggcaca
     5701 ggagtaactc tatctatggc atcaccttta cgcatcgtgc cactcggcgg gtgcggcgaa
     5761 atcggcaaga acatgacggc gttcgagtat gggcgcaaca tcctgctcat cgacgccggc
     5821 atcatgttcc ccgaaaacga catgttgggg atcgacttca tcatccccga ctggcagtac
     5881 ctgcgcgaca agaaagactt ggtccgcgcc atcgtcgtga cgcacggcca cgaggatcac
     5941 acgggcgcgc tgccatacct gctcaaagac ggattcaacg cgccgattta cgccacgaag
     6001 ctgacgcgcg ggctgctcga agtgaagctg aaacagcaca aggtcttcga agaggctaac
     6061 ctgaacacga tcgcggcggg cgaggcggtc accatcggcc cgttcaagat ggatttcttc
     6121 cgcgtagcac attcgatccc cgacggtgtg ggcttgggca tcaccacgcc ggccggcctc
     6181 gtcgtccata cgggcgactt caagttcgat cacacgccgg tcgacggcaa gccgaccgac
     6241 ttcgccaggc tggccgagtt cggcgggcgc ggcgtgctgg cgctgatgtc cgacagcacc
     6301 aacgccgacc agcccggcac gacgccgtcc gagcgcgtga tcgacgaagc cttcgatcgc
     6361 gtcttccgcg aagcgcaagg ccgcatcatc atcggcacgt tcgcgtcgct catctcgcgc
     6421 atccagcagg cggccaacgc cgccatgcgc tatcggcgca ggatggcgat cgccgggacg
     6481 agcatggccg agaacgtgcg catggcgcaa aagctgggtt acctgacctt tcccgacgac
     6541 ctgatcgtcc gcctcgacga ggcggcgcgc atgcgcccgc gcgacatcgt gatcatggct
     6601 accggcacgc agggcgagcc gtcgtcagtg ctggcgcgcg tggcggcggg agagcacagc
     6661 cagatcaagg tgcagcgcgg cgacacggtc gtcatgtcgt ctcacccggt gccgggcaac
     6721 gaagagatgg tgcaccgcac gatcaacagg ctcttccagc gcggcgccga ggtgctgtac
     6781 gatccgatcg aaatggtgca tgtgtcgggc cacgcctcgc aggaggaaca gaagctgatg
     6841 ctcaacctgg tgcggcctga gcatttcata ccgattcacg gcgagctgcg tcacctccac
     6901 gcgcacgccc ggctggcccg gctggtcggc gtcgagccga ccaacatcca cgtcgtcgag
     6961 aacggctatc cgatcgagtt cgacgaaggc gaggcgcgca tcggcgagcg cgtgccgggc
     7021 ggctacgtgt acgtcgacgg ctcgggcgtt ggcgacatcg gcccgagcgt gctgcgcgag
     7081 cgcgagatgc tcgggcgcga tggattcgtg atgacgatcg tccaccgcga tcccgagaac
     7141 ggccgcctgc tcgacacgcc cgacatcgtc acgcgcgggt tcgtctacgc ccgcgaagcg
     7201 caagacctgc tcgaaggcgc gacacaggcg gtgatccaaa tcgtcgaatc gaatggccgc
     7261 gccagccagg aagcgatccg cgacagggtc gagaagtcg
//
LOCUS       DBNHXB010000366         7157 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3961846, whole genome shotgun sequence.
ACCESSION   DBNHXB010000366 DBNHXB010000000
VERSION     DBNHXB010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3961846"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="AC162G_14940"
     CDS             <1..417
                     /locus_tag="AC162G_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR5077148.1"
                     /translation="EELRQHIREADQARCAYCLTSEANSGIPLTIDHIRPLSRGGQTV
                     FENLCLACRTCNEFKADRTDAEDPLSGDLCPLFNPRTQKWTDHFEWSADGTRMSGFTA
                     IGRATIIALRVNEEVIVAARRRWVLSGWHPPKDLQS"
     gene            complement(526..978)
                     /locus_tag="AC162G_14945"
     CDS             complement(526..978)
                     /locus_tag="AC162G_14945"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR5077149.1"
                     /translation="MITRSLVLRNDPIFNVMDRFVSDRFFAPLSREWATAWGIGSRLP
                     MDITEDGEGYTFVAVAPGLRAEDVHIETQGSTIRISGETIAPALSADEKVRTLRSEIG
                     YGKFSRTFELPEDIQADKIEARLEDGLLTVRVPKAEEVKPRTIKVQAK"
     gene            complement(1182..2654)
                     /locus_tag="AC162G_14950"
     CDS             complement(1182..2654)
                     /locus_tag="AC162G_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077150.1"
                     /translation="MKQVVTGILIVLIPAALIALGIGVYALAQQNQALQDELAESRAR
                     SADLQAQIEERDEEAARLHARIEELQAQIDALKQQLRDAGQEPVSVISLENAAAMDAI
                     EQAVQEMRGLAAIRPVTRTLMTSEELRAYVVDLQEKNYSQADARSDALTLAAFDLVEP
                     DFELYDFLTDLYSEQIAGFYDPDKDRLYVIAQPGDLSVLERITFAHEFDHALQDQHFD
                     LAGLGFSNDKALQIEDADRQLAYQALVEGDGALLMQQWAMDTLSLGDQLAMMSAAQDM
                     PVFASAPRVFRESLMFPYLSGMTFVLSLQLGGGWPAVDAAFDDPPVSSEQILHPSAYP
                     DDRPIPIALPPLTGTLPAGWRLADENTLGEFMLRQYLTEHISRTASVERAAEGWGGDR
                     YAVYVSPGAGAATPDTFEYVLVISQAWDSERDAEEFAEVYREYLRVRLDLDSSTRTQA
                     GIEWWIGPVTTYFAQRGDRTLIVAGPDEATVQPVVETVSP"
     gene            complement(2743..3891)
                     /locus_tag="AC162G_14955"
     CDS             complement(2743..3891)
                     /locus_tag="AC162G_14955"
                     /inference="COORDINATES: protein motif:HMM:NF052764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR5077151.1"
                     /translation="MNRSIHLAGAFLVVGALISACGGSPAAPASQATLPPVIDDAAVS
                     TEGRLVPVESVALSFGIGGEVAEVLVAEGDIVEAGDRIARLNDDSLQAAVAQAEAGLA
                     VVKANQAKYRETLPQQIAAAEAELSSAQAQLAEASAGRDSTAASIEAEAALAEARFAQ
                     QQAQTAYDRIIEAGLGGPVEERARLALESAVKTTQAAQARVDALKAGSPGDRADSAQI
                     AAAVAGVQAIQARLDQLKSEAAGTAVDTHAAAVQQAEAALLAAQVALAQVDLRAPMGG
                     TVARIDLKPGERVGPGAIVAVIADLSSWQVETDDLTEIDVPAVSVGQPVTVRVDALPD
                     VALKGEVASIGLLFQEKSGDVTYPVKIRLLGADPRLRWGMTVAVTFGK"
     gene            complement(3937..4593)
                     /locus_tag="AC162G_14960"
     CDS             complement(3937..4593)
                     /locus_tag="AC162G_14960"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077152.1"
                     /translation="MVKTFETAAGSFTALNGIDLKVNEGEFVAVIGKSGSGKSTLLNM
                     IAGIDRPTSGEVWVSDTAVHRLSENQMAKWRGLNVGVVFQFFQLLPTLSLVENVMLPM
                     DFRNVYAPRDREKRAMRLLEMVGVTEHARKLPSAISGGQQQRVAIARALANDPPIILA
                     DEPTGNLDSKAAESVFALFDDLVAEGKTVLMVTHDGELARRVRRAMVVSDGQVIDPFG
                     "
     gene            complement(4765..>7157)
                     /locus_tag="AC162G_14965"
     CDS             complement(4765..>7157)
                     /locus_tag="AC162G_14965"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077153.1"
                     /translation="RTTLAMKTGYRKTLRDVWRDKGRTILVVLSIGVGVFSVGLVSAM
                     NEMMPANMARSFRESNPAHITLWVSGLIDDDALRGLARVAGVTGVEGELRTTVRWRPD
                     PDTPWRDAPLIVRDDFTRQKFDRVELLAGEWPGDHEVVVEHNAVGFFGIPAGGSITIS
                     TGDNVRAVPVSGAVRDLQSFPPQFGGDAAFYANREFAAWLLGAGNFNRVKAQVPHFTQ
                     PSADDAAVALKERLLKIGYRSGTPEIQNPNEHFFQETVDTVILILGVMAVLSLALGLF
                     LVVNTIHAILAQQVSQIGVMKAVGAMTGQVARLYLAGVAMYGALALVIALPLGGLAAH
                     AVSGALLRLLNITIEPFRFSATAVSQQIGTALIAPGLAALWPIASGARVTVREAIASY
                     GIGLDYGASLFDRLLARVRGLPRPLMLTLRNTFRRKARVALTQITLTIAGLVFIMVAS
                     LGQSLNHTLDTFIDTLGLDVALFFSEDVRVDEAEALINRQPGVAQAEMWLFRSGAAKR
                     RADDVAGERIVLRAVPPDTRLYRPSIVRGRWLHPQDGHALVINQVLAGDLGVSVGDTL
                     VVELGEERVIEWTIVGAVFDLSNNQAASYVPRDVLLRDIGRVGRTSIAWMRTTQHDGA
                     FQLRVASQLRDLFQARGITVGGTQTAGEIAESNRSQFNILLTLLLVMSGLIALVGSIG
                     LAGTLSINVLERRREIGVMRAIGASSWSVAALFVGEGILLGLIAWASAVPLSVPGGNL
                     FASAIAGALEVPMVYQFSWSGAAIWLAIMLALSIVASSVPALRATRLSVRESLAYE"
BASE COUNT         1248 a         2404 c         2257 g         1248 t
ORIGIN      
        1 gaagaattgc gtcagcatat tcgtgaagct gatcaggctc gctgcgctta ctgcctgacg
       61 agtgaggcca atagcggaat tccactcact atcgatcata tcaggccgct gtccagagga
      121 ggccagacgg tttttgagaa cctgtgtctg gcgtgtcgca cctgtaatga gttcaaggcg
      181 gatcggacag atgccgaaga cccattaagt ggcgacttgt gccctctgtt taatccgcgc
      241 actcagaaat ggactgatca cttcgagtgg agcgccgacg ggacgcgcat gagcggtttt
      301 acggcgatag gccgggcgac gatcatcgcg cttcgtgtga atgaagaggt tatcgttgcg
      361 gcgcgtcgcc gatgggtgct cagcggttgg catccgccga aagacctgca aagctgacgg
      421 tcggatttgg cccgcctgtc tgcacttcat gacgcgctgg accctgtaac gcaaagagcc
      481 gcccccattg ggacggccct ccaatctcca atctccaatc tcaaattact tggcttgtac
      541 tttgatcgtg cgcggcttga cttcctcggc cttcggcacg cgaaccgtca gcagcccatc
      601 ctccagcctg gcctcgatct tgtcagcctg gatgtcctcg ggcagctcga atgtacggct
      661 gaacttgccg tagccgatct cgctgcgcag cgtgcggacc ttctcgtcgg ccgacagcgc
      721 gggcgcgatc gtctcgccgc tgatgcggat cgtgctgccc tgcgtctcga tgtggacgtc
      781 ctcggcgcgc aggcccgggg cgacggcgac gaaggtgtat ccctcaccgt cctcggtgat
      841 gtccatcggc agccgcgagc cgatacccca cgccgtggcc cactcgcgcg acagcggggc
      901 gaaaaagcga tcgctcacga agcggtccat gacgttgaag atcggatcgt tgcgtaacac
      961 cagacttcga gtaatcattt cgatatcctc cttggtaggt ctcctgtctg taaactgcgc
     1021 ccaggatacc cgctttgcat tagagcgacg caagcggcac gctagatgag cattagaggg
     1081 gcgttagagt tgtattggag ggcgacgaaa gacggacgac catagacgaa tgccacgaca
     1141 ttcgtcgttt gtctttcgtc cttcgtcatg cagtgggctg ctcaggggga aaccgtctcg
     1201 actaccggct ggacggtcgc ttcgtccggc ccggcgacga tcaatgtgcg gtcgccgcgc
     1261 tgagcgaagt acgtcgtcac cggcccgatc caccattcga tcccggcttg tgtccgcgtc
     1321 gagctgtcca ggtcgagtcg gaccctgagg tattctcggt agacctcggc gaattcctcg
     1381 gcgtcgcgct ccgaatccca tgcctgcgag atgacgagca cgtactcgaa ggtgtccggc
     1441 gtggccgcgc cggcgccagg cgagacgtac acggcgtagc ggtcgccgcc ccaaccctca
     1501 gcggcccgtt cgacggatgc cgttctgctt atgtgctcgg tcaaatactg gcgcagcatg
     1561 aactcgccga gtgtgttctc gtcggccaat cgccagccgg cgggcagcgt gccggtgagc
     1621 ggcggcagcg cgatcgggat cggtcgatca tccgggtagg cagacgggtg cagaatttgc
     1681 tccgacgaga cgggcgggtc gtcgaaagcg gcgtccaccg ccggccagcc gcctccgagt
     1741 tgcagcgaca gcacgaaggt catgccgctc aggtagggaa acatcagcga ttcgcgaaac
     1801 acgcgcggcg ccgaagcgaa aaccggcatg tcttgcgctg cgctcatcat cgccaactga
     1861 tcgccgagcg acagtgtatc catcgcccac tgctgcatga gcagcgcgcc gtcgccttcc
     1921 accagcgcct ggtacgcaag ctgccgatcg gcgtcctcga tttggagggc cttgtcgttg
     1981 ctgaagccca ggccggcgag gtcgaaatgc tggtcctgca gcgcgtggtc aaactcgtgc
     2041 gcgaaagtga tccgctccag cacggagaga tcgcccggct gagcgatcac gtacagccgg
     2101 tccttgtcgg gatcgtagaa gccggcaatt tgctcgctgt acaggtcggt caggaaatcg
     2161 tacagctcga aatccggctc gaccaggtcg aacgcggcca gcgtcagcgc atcgctgcgc
     2221 gcgtccgcct ggctgtagtt cttctcctgc aagtcgacga cgtaggcgcg aagctcctcg
     2281 ctcgtcatca gcgttcgcgt gaccggccgg attgcggcca gcccgcgcat ctcctgaacg
     2341 gcctgctcga tcgcgtccat cgccgcggcg ttctcgagtg agataactga gacaggctct
     2401 tggccggcgt cgcgcaattg ctgtttgagc gcgtcgatct gcgcctgcag ctcctcgatc
     2461 cgggcgtgca gcctcgccgc ttcctcgtcg cgctcctcga tctgcgcctg cagatcggcc
     2521 gagcgggcgc ggctctcggc caactcgtct tggagcgctt ggttctgctg agccagcgcg
     2581 tacacgccga tgcccaaggc gatcagcgcg gcgggaatca acacgatcaa gatgccggtg
     2641 acgacttgct tcacgatggc tccctgcgcg gcgatggtag catggcgcga tgagggtgtc
     2701 aaaccgttca cgttttacgc ttcacgtttt acgcatcacg tttcacttcc caaacgtcac
     2761 cgccacagtc atcccccacc gcagtcgcgg atccgccccg agcaacctga tcttcaccgg
     2821 gtaggtcacg tcgccgctct tttcctggaa cagcaggccg atcgaggcaa cctcgccctt
     2881 cagcgccacg tccggcaggg cgtcgactct caccgtcacc ggctgcccga cgctcacggc
     2941 gggcacgtcg atctcggtca gatcgtcggt ctccacctgc caactggaca ggtcggcgat
     3001 cacagcgacg atcgcgcccg gcccgacgcg ctcgccgggc ttaaggtcga tgcgcgccac
     3061 cgtcccgccc atcggcgctc gcagatcgac ctgcgccagg gcaacctgcg cggcgagcag
     3121 cgcggcctcg gcctgctgca cggcggcggc atgcgtgtcg acggctgtgc ctgctgcttc
     3181 ggacttcaat tgatccagcc gcgcctgaat ggcttgcacg ccggcgacag ccgccgcaat
     3241 ctgcgcgctg tcggcgcgat cgccgggcga gccggccttc agcgcgtcga cgcgcgcttg
     3301 ggccgcctgc gtcgttttga ccgccgactc cagcgccagc cgcgcccgct cttcgaccgg
     3361 gccgcccagc ccggcctcga taatgcgatc gtaagcggtc tgcgcctgct gttgcgcgaa
     3421 tctcgcttcg gccagcgcgg cctcggcttc gatcgacgcg gcagtggagt cgcgcccggc
     3481 ggatgcctcg gccaactgcg cctgcgctga gctgagctcc gcttcggcgg cggcgatctg
     3541 ctgcggcagc gtctcgcgat atttcgcctg gttcgccttg accaccgcca gccccgcctc
     3601 agcctgcgcg acggcggcct gcagcgagtc gtcgttcagc cgggcgatcc gatcgccggc
     3661 ctccacgatg tcgccttctg ccaccagcac ctcggccacc tcgccgccga tgccgaagct
     3721 cagtgcaacg gattctaccg gcacaagccg gccctctgtg ctcacggcgg cgtcgtcgat
     3781 tacgggcggc agcgtcgctt gggacgcggg cgctgcaggc gatccgccgc acgccgagat
     3841 gagcgcgccc acaaccagga aagcgccggc aagatgaata cttctgttca tgctaaacct
     3901 cttcaagaat gacgtgcgaa aaaggagacg aattgatcac ccgaagggat cgatgacctg
     3961 cccgtcggac acgaccattg cacgccggac gcgccgggcc agctcgccgt cgtgcgtgac
     4021 catcaagacc gtcttgccct cggcgaccag atcgtcgaag agcgcgaaaa ccgactcggc
     4081 cgccttggaa tccagattgc cggtcggttc gtcggccaga atgatcggcg ggtcgttggc
     4141 caacgcacgg gcgatggcga cgcgctgctg ctgaccgccg gaaatcgccg agggcagctt
     4201 gcgggcgtgc tcggtcaccc cgaccatctc gagcaggcgc atggcgcgct tctctcggtc
     4261 acggggcgcg tacacgtttc ggaagtccat gggcagcatc acgttctcga cgagcgacag
     4321 ggtgggcagg agctggaaaa actggaagac gacgcccaca ttgaggccgc gccacttcgc
     4381 catctggttc tcgctcaggc gatgcacggc ggtgtcgctg acccacacct cgcccgaagt
     4441 cggccgatcg atgccggcga tcatgttaag caaggtggac ttgccgctgc ccgatttgcc
     4501 aatgacggcg acgaattcgc cctcgttgac tttcaggtcg atgccgttga gcgccgtgaa
     4561 cgacccggcg gcggtctcga aggtctttac cacttgccgc agctcgatga gacgcgtatt
     4621 gccgttgcgg accgtggact cgctagattt cgctgcatgc ccaaccatgt attctccctc
     4681 cctcgaatca acccggcccg atactgagcg atacgcgaca cgcgatatgc gatatgcgat
     4741 acgcggtatg cgatatgcgc caggctactc gtaggccagg ctctcgcgca cgctcagccg
     4801 cgtcgcgcgc agcgcgggca cgctgctggc gacgatcgac agcgcgagca taatcgccag
     4861 ccagatcgcc gcgccgctcc acgaaaactg gtagaccatc ggcacttcga gcgccccggc
     4921 gatggcggag gcgaacaaat tcccgcccgg cacgctcagc ggcacggccg acgcccaggc
     4981 gatcaggccc agtaatatcc cttcgccgac gaacagcgcc gccacgctcc acgacgacgc
     5041 gccgatcgcg cgcatcacgc cgatctcgcg ccggcgctcc agcacgttga tcgacagcgt
     5101 gccggccaga ccgatactgc ccaccagcgc gatcaggccg gacatcacca gcagcaatgt
     5161 gagcaggatg ttgaactggc tccggttcga ttcggcgatc tcgcccgcgg tctgcgtgcc
     5221 gccgacggtg atgccgcgcg cctggaacag gtcgcgcaac tggctcgcga cgcgcaactg
     5281 aaacgcgccg tcgtgctgcg tcgtgcgcat ccaggcgatg gaggtgcgcc caacccggcc
     5341 gatatcgcgc aggagtacgt cgcgcggcac gtaagacgcg gcctggttgt tgctcaggtc
     5401 gaacacggcg ccgacgatcg tccattcgat gacgcgctcc tcccctagct cgacgacgag
     5461 ggtgtcgccc acgctcacgc ccaggtcgcc ggccagcacc tggttgatca cgagcgcgtg
     5521 cccatcctgg ggatgcagcc agcgaccacg cacgatggat ggccgataaa gccgggtgtc
     5581 cggcgggacc gcgcgcaaga caatgcgctc tcccgccacg tcgtcggcgc ggcgcttcgc
     5641 ggcgccggat cgaaagagcc acatctcggc ctgcgctacg cccggctgcc ggttgatgag
     5701 tgcttcggcc tcgtcgacgc gcacgtcttc cgagaaaaac agcgccacgt ccaacccaag
     5761 cgtatcgatg aacgtgtcga gtgtatggtt gagcgactgg ccgaggctgg cgaccatgat
     5821 gaacaccagc ccggcaatgg tcagcgtgat ttgcgtcagc gccacgcgcg ccttgcgccg
     5881 gaaggtgttg cgcagcgtca acatcagagg gcgcggcagc ccgcgcacgc gcgccagcag
     5941 ccggtcgaac agactcgcgc cgtagtccaa cccgatgccg tagctggcga tcgcttcacg
     6001 cacggtgacg cgagcgccgc tggcgatcgg ccacaaagca gccagccccg gcgcgatcag
     6061 cgccgtgccg atctgctgcg agacggccgt cgccgagaac cgaaacggct cgatcgtgat
     6121 gttgagcagc cggagcagcg cgccgctcac ggcgtgcgcg gcgaggccgc ccagcggcag
     6181 ggcgatcacc aacgccagcg cgccgtacat cgccacgccg gccagataca ggcgcgccac
     6241 ctggccggtc atcgcgccga ccgccttcat cacgccgatt tgggagactt gctgcgcgag
     6301 gatcgcgtgg atggtgttga cgaccaggaa cagtccgagc gccagcgaaa gaaccgccat
     6361 cacgccgagg atgaggatca ccgtatcgac cgtttcttga aagaagtgct cgttcggatt
     6421 ctggatttcc ggcgtgcccg atcgataacc gatcttcagc aatcgttctt tgagcgccac
     6481 ggccgcgtcg tcggctgacg gctgcgtgaa gtgaggcacc tgggccttca cccggttgaa
     6541 attgcccgcg ccgagcagcc aggcggcgaa ctcgcggttg gcgtagaacg ccgcgtcgcc
     6601 gccgaactgc ggcggaaagc tctgcaggtc gcgcaccgcg ccgctaaccg gcacggctcg
     6661 cacgttatcg ccagtcgaga ttgtgatcga gccgccggcg ggaatgccga agaagccgac
     6721 tgcgttgtgc tctacgacga cctcgtgatc gcccggccac tcgccggcga gcagctcgac
     6781 gcgatcgaat ttctggcgcg tgaagtcgtc gcgcacgatg agcggtgcgt cgcgccacgg
     6841 cgtgtcaggg tcgggtcgcc agcggacagt cgtgcgcagc tcgccttcga cgcctgtcac
     6901 gcccgcgacg cgcgcaagcc cgcgcagcgc gtcgtcgtcg atcaggccgg atacccataa
     6961 cgtgatgtga gcgggattcg actctcggaa actgcgcgcc atgttggccg gcatcatctc
     7021 gttcatggcc gacaccaggc ctacggagaa cacgccgacg ccgatgctca gcacgacgag
     7081 gatcgtgcgc cccttgtcgc gccacacgtc acgcagcgtc tttcggtagc cagtcttcat
     7141 agcgagtgta gttcggt
//
LOCUS       DBNHXB010000367         2365 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_103635, whole genome shotgun sequence.
ACCESSION   DBNHXB010000367 DBNHXB010000000
VERSION     DBNHXB010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_103635"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            472..1962
                     /locus_tag="AC162G_14970"
     CDS             472..1962
                     /locus_tag="AC162G_14970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5077154.1"
                     /translation="MRRRAFCVSSAAALGAAAFPFGRAFSVAATVTTDVEAVTGDGRR
                     ILLSRSDVEEFRAGLRGQLLLRDAAGYDDARKIFNGMFDRKPAMIARCLGAADVVRSV
                     RFAQAHGVLVAVRGGGHSLSGQSVCDDGLMIDLAPMRGVRVDPVRRLARAAGGALLGD
                     LDRETLAFGLVTTTGTVSHTGVGGLTLGGGFGRLARRYGLTCDQLTSVDIVTADGQLL
                     EASATANDDLFWGVRGGGGNFGVVASFEYRLHPFDGWATGGHLVFPFEQARQALRGFA
                     ELYPRAPEGLWIEPILTTTPGGARVLILDVCCTDPPAAAEAALVPYRKLGKAIEDGVK
                     RVRYTVLQTKDDERARIGGRYYTKSGFTNTLDDGLIDLMLDTIGGATIPRARIAMPPK
                     GGAIERVAPDATAFWHRAPLYSVLLQTSGDEFAEDAANLAWAKAKWPALEAHTKGFYA
                     NTNLSEMPADRVGEAYGGNQARLAELKTRYDPTNLFRLNANVRPRA"
     gene            complement(2122..>2365)
                     /locus_tag="AC162G_14975"
     CDS             complement(2122..>2365)
                     /locus_tag="AC162G_14975"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016893 - obsolete endonuclease activity,
                     active with either ribo- or deoxyribonucleic acids and
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HIR5077155.1"
                     /translation="DWLIPMSESHLRSILRAWTGHYKRGRPHMALGPAVADPPLVIVP
                     PATPLTRHRIGERLGVCARSVLGGLHHEYLAAPALA"
BASE COUNT          417 a          755 c          777 g          416 t
ORIGIN      
        1 ctgcataaca ccgtccagag tcacgaaagt acccacaacc agttttctca tattcgtcat
       61 ccccgccgcg ccgagtcgaa ggtggcggcg tcagccatca atggccccgg atagtccacg
      121 ggcgataccc ggaacccgtt tcgaatcatc aagtgagcta atcgacagat agctacggaa
      181 catttctcct cgaaggcgag aagatggcgc cgctgtgccg cgaatttcgg cacctcccgg
      241 gtcaccggat acaagagctt cgagcgctac aacacctgcg gtctcgacgg gctctactac
      301 cgcagcaagg ccccctaccg gcaggccaac aaactgccgt tccaggttgt ctatgcgcag
      361 ccgatgcaag gaagttccga tcgcccggct gccgcgatga ccgccgactt cccttgcttg
      421 cgtggcaccc tgccccggcg cagagtggca cgctctggag ggggacggac catgagacga
      481 cgggctttct gcgtgtcctc tgcagctgcg ctgggtgcag cggcgtttcc tttcggtcgt
      541 gctttctcgg tggcggccac ggttaccacc gatgtggagg ctgtcaccgg cgacggccgg
      601 cgaatcctgt tgtcgaggtc ggacgttgag gagttccgcg cgggcctgcg cgggcaactg
      661 ctgttgcggg atgcagcggg ctacgacgac gcacgcaaga tcttcaatgg catgttcgat
      721 cgcaagcccg cgatgatcgc gcgctgcctg ggtgccgcgg acgtcgttcg ctcggtgcga
      781 ttcgcgcagg cgcacggtgt actcgtcgcc gtgcgcggcg gcggacacag cctctcgggg
      841 cagtccgtct gcgatgacgg cctgatgatt gacctggcgc cgatgcgcgg tgtgcgcgtc
      901 gaccccgtgc gccggctggc gcgcgccgcc ggcggcgcgc tgctcggcga tctcgatcgc
      961 gaaacgctgg cgttcggcct ggtcactaca accggcacgg tctcgcatac cggcgtcggc
     1021 gggctgaccc tgggtggcgg gttcggacgc ctggcacgcc ggtacggcct gacctgtgat
     1081 cagctgacgt cggtggacat cgtcaccgca gatggccagc tgctcgaggc aagtgccacg
     1141 gcgaacgacg acctgttctg gggcgtacgt ggcgggggcg gcaattttgg cgtcgtcgcg
     1201 tcattcgagt atcggctgca cccgttcgac ggctgggcga ccggcggaca cctggtgttc
     1261 cccttcgaac aggcgcgaca ggccctgcgc gggttcgcag aactgtaccc gcgcgcgccc
     1321 gaaggacttt ggattgagcc catactgact acaacgccgg gtggcgcgcg cgtgctgatc
     1381 ctggacgtct gctgcactga tccccctgcg gcggccgagg ctgcgctcgt gccttatcgc
     1441 aaactaggga aggcgatcga ggatggcgtg aagcgggtgc gatacacggt gttgcagacg
     1501 aaggacgacg agcgcgcgcg catcggcggc cgctattaca cgaagtcggg atttacgaac
     1561 acgttggacg atggcctgat tgacctgatg ctggacacga tcggcggcgc cacgattccg
     1621 cgggcgcgca tagcaatgcc gccgaagggc ggtgcgatcg agcgcgttgc tcccgacgcc
     1681 actgcattct ggcatcgcgc cccactgtac agcgtgctcc tgcaaacgag cggcgacgag
     1741 ttcgccgaag atgcggcaaa tcttgcctgg gccaaggcga agtggccggc gttggaagct
     1801 catacaaagg gcttctacgc aaatactaac ctgtcggaaa tgccagccga tcgggtcggc
     1861 gaagcctacg gcggcaacca agcgcgattg gccgaactca agacccgcta cgacccgacc
     1921 aacctattcc gcctcaacgc gaatgttcgc ccgcgcgcct gatgcacgca tcaaccgtta
     1981 cggagggacc cgttgcagac tgcgcagata acgtggtttg gcctcgacgg agaacgttgg
     2041 acgtatctcg cccgcgtgcc aagtgctcga taggcttgcc acgaaggccg cgagattcgg
     2101 ctgtgctccg caattacttg atcacgccaa cgcgggcgcc gccagatatt cgtgatgcaa
     2161 gccacccagc accgatctgg cgcacacgcc gaggcgctcg ccgattcgat ggcgggtgag
     2221 tggtgtcgca ggcgggacga tcaccaatgg agggtccgcc acggctgggc cgagcgccat
     2281 gtgcgggcgt ccgcgtttgt aatgacccgt ccatgccctc aggatcgacc gcaggtgcga
     2341 ctccgacatc ggtatcaacc agtcc
//
LOCUS       DBNHXB010000368         3625 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4465296, whole genome shotgun sequence.
ACCESSION   DBNHXB010000368 DBNHXB010000000
VERSION     DBNHXB010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4465296"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="AC162G_14980"
     CDS             complement(<1..1421)
                     /locus_tag="AC162G_14980"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR5077156.1"
                     /translation="MPIEDLSVTPSGILLLALLGIAVLFFITEWLRADLVALLLLVAL
                     GATGILTQQEALSGFSRSAVITILAIFILTAGLARTGATRALSRHLMRWGGRGELSLI
                     VVLMTAGALLSLFMNNIAAAAVLLPVAVGISRERKISPSKLLMPLAFGTILGGMATLL
                     TTSNILVSAALRDANYPGFGLLDFAPIGVPLVVVGILYMTLIGHRLLPRRAPADWTRL
                     LTTSQLQLAEVYGLGERWVCAHVPETSPLAGRTLSEVGLGRKLGVNVVAVLHQTKARL
                     APPPTERLCVGDEVYVQAREEQIGELRTRGLAIRPGPASLDRLMSEKAGLFEVVLAPR
                     SGAVGKTLRAIHFREKYDLSVIAIWRKGRPRRVGIADIPLQLGDALLVLGPRDRARVV
                     QSEGDFIVLADTVDESLRTARMPWAIAIMAVTIVVAAVGGLPTGEAMLAGALAMVLTG
                     ALTMDEAYRSIEWKSIFLIAAMLP"
     gene            complement(1576..2595)
                     /locus_tag="AC162G_14985"
     CDS             complement(1576..2595)
                     /locus_tag="AC162G_14985"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR5077157.1"
                     /translation="MTKTIRVCVLTQSPMPDPLLESLRAVSPRLIVEHRTAQTLKELG
                     DAVWPGVEVLYTTMLMPSPEQAPDLRWVQGHFAGVEQFLDHPLFQRAALTTTSGIHAP
                     AMAEYILMMMLAFAHHLPRMIYYQKRSEWPKGRWDLFAPRELRGSTAGIVGYGSIGRE
                     VARLARAFGMRVLATKRDVAHTGDTGWGLPGIGDAAAQHVDRLYPADALHAMLAECDY
                     VALSVPLTPETHHLIGEAELERMKPDAVLINVSRGGVIDEAALAEALRAGTIRGAALD
                     VFEQEPLPASSPLWSLPNVILSPHVSGFTFSYDERAMALFAENLRRYVNGEELLNRVD
                     LRRGY"
     gene            complement(2588..>3625)
                     /locus_tag="AC162G_14990"
     CDS             complement(2588..>3625)
                     /locus_tag="AC162G_14990"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR5077158.1"
                     /translation="NLKKNAPAWEGMSPDYWDDLDSEETLEAITNALEARGHKVTFLE
                     GDVTLYNNLLKLRPDICFNICEGHFGDGREAQVPAILEMLRIPYTGSKVLTLALALDK
                     PMTKRVLTYHELPTPLFQVFERVDEPLHPSMQFPLFVKPSREGTGMGVTSESILRSEE
                     DLRTQLPRIFERYQQPALVEKFVEGREVTLGVVGNLVLPAARRVPEDEHAPRVFEGLH
                     FFPPLEVDTERYPEEEGIYSSRLKVELAHEFYYLCPANLTEAQLNELQFLTAAVFRIT
                     GGLDVARVDFRLDRHAGDKPYILEINPLPGLNPGYSDLCIEAEADGWTHEELVNRILD
                     EAIKRYGLKHD"
BASE COUNT          662 a         1203 c         1132 g          628 t
ORIGIN      
        1 ggcagcatcg cggcgatcag gaaaatgctt ttccattcga tcgaccggta cgcttcgtcc
       61 atggtcagcg cgcccgtgag caccatcgcc agcgcgccgg ccagcatcgc ctcgccggtc
      121 ggcagcccac ccacggcggc cacgacaatc gtaaccgcca tgatcgcgat ggcccacggc
      181 atccgggctg tgcgcaggct ctcatcgacg gtatcggcca gcacgatgaa atcaccttcg
      241 ctctgcacga cacgcgcccg atcgcgtggc ccaagcacga gaagcgcgtc acccagttgc
      301 agcgggatgt cggcaatacc gacccgccga ggacgccctt tgcgccagat ggcaatcacg
      361 ctcaggtcat acttctcgcg gaagtgaatg gcgcgcaacg tcttgcccac cgcgcccgag
      421 cgcggcgcga gcacgacctc gaacaggccg gccttctccg acatcagccg gtcgagcgag
      481 gcgggtccgg gtcttatcgc caggccgcgc gtgcgcaact ccccgatctg ctcctcacgc
      541 gcctgcacgt acacctcgtc gcccacgcac aaccgctccg tgggcggcgg tgcgagacgc
      601 gctttggtct ggtgcagcac ggcaacgacg ttgacgccga gcttacggcc caggccaacc
      661 tcggacaacg tgcgtccggc cagcggcgaa gtctccggca cgtgagcgca cacccagcgc
      721 tcccccaagc cgtacacctc ggccaactgc aactgcgagg tcgtcagcaa ccgcgtccaa
      781 tcggcgggcg cgcggcgcgg cagcaagcgg tggccgatca gggtcatata caggataccc
      841 accaccacta agggcacgcc gatcggcgcg aagtcgagca gaccgaatcc cggataattt
      901 gcgtcgcgca gcgccgcact gaccaggatg ttcgaggtgg tcagcagtgt cgccatgccg
      961 cccaggatcg tgccgaaggc gagcggcatc agcaacttcg agggactgat cttgcgctcg
     1021 cgcgaaatgc cgacggcgac cggcagcagc accgccgccg cggcgatgtt attcatgaag
     1081 agcgacagca gcgcaccggc cgtcatcaaa acgacgatca ggctcaactc gccgcgcccg
     1141 ccccagcgca tcaagtgccg gctcagggca cgcgtcgcgc cggtgcgcgc caggccggcg
     1201 gtgagaatga agatggcgag gatggtgatg acagcggatc ggctgaagcc ggagagcgct
     1261 tcctgttggg tgaggatgcc ggttgcgccg agcgcgacga gcaggagcag tgcgaccaga
     1321 tcggctcgta gccattcggt gatgaagaat aacaccgcaa tgccgagcaa cgctaacaat
     1381 aggataccgg atggcgtcac gctgagatcc tcgatcggca agataccacg ctccaagccg
     1441 gttctgcgcc gccggcgatt atatccggtt cttccattct cgaagtggcc ttcaggttta
     1501 tgatgaaccg ggcgtcccgc gtggtcaacg gcgctacttt caaaacgtgc gcttgaccca
     1561 agctggcctg tgggcttagt atccccttcg caggtcaacc ctgttcagca gctcttcgcc
     1621 attcacgtag cgcctgaggt tctcggcgaa cagcgccatc gcgcgctcgt cgtacgagaa
     1681 tgtgaagccg gacacgtgcg ggctgaggat cacgtttggc aagctccaca gtgggctcga
     1741 agcgggcaga ggctcttgct cgaagacgtc gagtgccgca ccgcgaatcg ttcccgcgcg
     1801 cagcgcctcg gccagcgccg cttcgtcgat cacgccgccg cgcgagacgt tgatcagcac
     1861 cgcgtccggc ttcatgcgtt ccagctcggc ttctccgatc aggtggtgcg tctctggcgt
     1921 cagcggcacg ctcagcgcga cgtagtcaca ctcggccaac atggcgtgga gcgcatcggc
     1981 cggatacagc cggtccacgt gctgggccgc tgcatctccg ataccgggca gtccccagcc
     2041 tgtgtcgccg gtgtgcgcca cgtcgcgctt ggtcgccagg acacgcatgc cgaacgcgcg
     2101 cgccagccgt gccacttcgc gcccgatgct gccgtatccg acgatgccgg cagtcgatcc
     2161 gcgcagctcg cgcggcgcga acaggtccca gcgtcccttg ggccactccg accgtttctg
     2221 ataatagatc atgcgcggca gatggtgcgc gaaggcgagc atcatcatca ggatatactc
     2281 ggccatcgcc ggcgcgtgga tgccgctcgt cgtcgtgagc gccgcgcgct gaaatagggg
     2341 atggtcgagg aactgctcga cgccggcgaa gtgcccctgc acccaacgca ggtcgggtgc
     2401 ctgctccggc gacggcatca gcatcgtcgt gtacagcact tcgacgccgg gccagaccgc
     2461 gtcgcccaat tctttgagtg tctgggctgt acgatgctcg acgatgaggc ggggagagac
     2521 ggcccgcagg ctctcgagca gcgggtcggg cataggggat tgagtgagga cgcagactct
     2581 gatggtttta gtcatgtttc aatccatatc gtttgatcgc ctcatccaaa atccgattga
     2641 ccaactcctc gtgtgtccag ccgtccgcct cggcttcgat gcacagatcc gaatagccgg
     2701 gattcaagcc gggtagaggg ttgatctcca gaatataagg cttgtcgccg gcgtgcctgt
     2761 ccagccggaa atcgacgcgc gccacgtcga ggccgcccgt gatgcggaat accgccgccg
     2821 tcaggaattg cagctcgttc aactgcgcct cggtcaggtt cgcgggacac aagtagtaaa
     2881 actcgtgagc cagctcgacc ttgagccggc tagaataaat gccttcctcc tctggatacc
     2941 tctccgtatc gacttcgagg ggcgggaaaa agtgcagacc ctcgaatacg cgaggtgcgt
     3001 gctcgtcttc cggcacgcgc cgcgcggcgg gcaggaccag gttgccgacg acgccgagcg
     3061 tgacctcgcg cccttcgacg aacttctcga cgagcgcggg ctgctggtag cgctcgaaga
     3121 tgcgtggcaa ctgcgtgcgc aggtcttcct cgctgcgcag gatcgattcg ctcgtcacgc
     3181 ccatccccgt tccctcgcgg ctcggcttga cgaacagcgg gaactgcatc gacgggtgca
     3241 gcggctcgtc gacgcgctcg aacacctgga acagcggcgt cggcagctcg tgatacgtca
     3301 atacccgctt ggtcatcggc ttgtcgagcg ccagcgccag cgtcagcact ttcgatcccg
     3361 tgtaggggat gcgcagcatc tcgaggatcg ccggcacctg cgcctcgcgc ccgtcgccaa
     3421 agtggccctc gcagatgttg aagcaaatgt cgggcctgag cttcagcaag ttgttgtaga
     3481 gcgtgacatc tccttctaga aacgtcacct tgtgcccgcg cgcttcgagc gcgttcgtga
     3541 tcgcctcgag ggtctcctcc gaatccagat cgtcccagta gtcgggcgac atgccttccc
     3601 aggcgggcgc gttcttcttc aagtt
//
LOCUS       DBNHXB010000369        15954 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_775773, whole genome shotgun sequence.
ACCESSION   DBNHXB010000369 DBNHXB010000000
VERSION     DBNHXB010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 15954)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15954)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15954
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_775773"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="AC162G_14995"
     CDS             complement(<1..921)
                     /locus_tag="AC162G_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077159.1"
                     /translation="MKARWLRLALALAIVLVALGALVVLGGIALARNPDAVSNAPPAA
                     AAVVDAGAPDAQASTAESDPLVLSYAAKFVCQEPLQPGQFYYGTAAPLVEEQTGVLVH
                     NSQPYTVTLYKKAVRARLETAPEIRPGAWVSVTLKPDHAIRIDCDDIAKLLTGSPAAT
                     FIGTYGIGVEVEGFVVILVGPQTVAGSNVARFGPVDVTAEYVRASEVLKKDISYQPWW
                     WWWWWPLPWRLGYAYQRILTIDPATNIDCRGVLYNALLQDAQAIADTQQRTLTLAALN
                     AGQNTDPTKPTRGGQDSPALVALIGRCDKID"
     gene            1259..1915
                     /locus_tag="AC162G_15000"
     CDS             1259..1915
                     /locus_tag="AC162G_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5077160.1"
                     /translation="MNEPALIAAARKGDLDAFNTLVLAYQHQVYNLAYRIMGDEASAA
                     DATQEAFISAYKHMESFRGDSFKSWIFRIVTNACYDELRRRKRRPASSLEGLVGDEDL
                     EIEYDIPDESETPETRVERRELAQVIEFGIGTLPPDQRITLVLSDMQGMSYHEIAAIT
                     GANLGTVKSRLSRARAKLRDYLVASGELSPGSSRPRTRQASAQPTPPGPAPSQAQGKL
                     "
     gene            1985..2785
                     /locus_tag="AC162G_15005"
     CDS             1985..2785
                     /locus_tag="AC162G_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR5077161.1"
                     /translation="MGLFGRKLTDQERAEELFSPYIDGQVTAGEKQFLERYLADHPEA
                     HEKFELLQAAVQMTKTLPPVKAPRSFVLPRSMARKPSLAVRMYPVLRFATVAAMALFA
                     FALIGDLTTSSRLQLAAPAREMVLSERSRELTEPAASAPAAQLFAATPAAMPTATPAP
                     QATPAPGAVGAPANVDATTAAEEPAPPSEQATAIADSRGAHLEQPTPTVEASADASAM
                     KAEEPESISITEQPPPVQVDALRLAVLALAGLAAVLGVTTLIIRRRVR"
     gene            2973..3392
                     /locus_tag="AC162G_15010"
     CDS             2973..3392
                     /locus_tag="AC162G_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR5077162.1"
                     /translation="MFFQEPKVAAGALIERDGAVLLVRRSVDPRRGAWALPAGYMEID
                     EGPVAATIRECFEETGLVVRVTGLFGVYHIANDPRGAGVLILYRASAESGELRPGDDA
                     SEVAYFGPDELPRDIAFASTRRVLARWCAERRMRDEE"
     gene            3512..4621
                     /locus_tag="AC162G_15015"
     CDS             3512..4621
                     /locus_tag="AC162G_15015"
                     /inference="COORDINATES: protein motif:HMM:NF015755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR5077163.1"
                     /translation="MTMNEQTTRPSRAVRRPAPSARPARTPFSLIALSGLLGVVVLAC
                     AMCSVMLAALGSDRVLALRDQVLGRTREERIASLQQIVDVVVRGEDAWPIPGRGRLTI
                     LLMGVDRRPSEGRAPARTDAITLLTVDPEAKTAAMLSIPRDLYVPLAGLDRVDRINAA
                     YVYGEARGVPGGGAQATEDTIAWNLGIPIDKYAVVDFAGFKQAIDALGGIDVDVPRRI
                     VDDTYPTEDFGIELLVIEPGPTHMDGELALKYVRTRHADSDFGRLQRQQQVMLAARDK
                     ALSLGALTQLPALLDAAAGSYETDLTVAEIASVAKVLSDVPRENIAAFRIDETMAQLW
                     ATPTGASVLIPLRDRIAPVVAAFLAHGQAQSSAAP"
     gene            5165..6346
                     /locus_tag="AC162G_15020"
     CDS             5165..6346
                     /locus_tag="AC162G_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5077164.1"
                     /translation="MSHTQLTVYGATWCSDCKRAKKFLGEQRVHYNWVDIEQDAGGLA
                     LVERVNDGKRIIPTIIFEDGGMLVEPSNAELAARLGLQTTASMSYYDLIVVGGGPAGL
                     TAALYAAREGLDVLVIEKSGLGGQAGVTERLDNFPGFPDGINGGDFADRLAQQARRFG
                     VEILQAQDVVGLRAEEESRYVQTADGNEYGARAVLIATGSTYRRLGVPGEDELIGAGI
                     HFCATCDGPFYKGQHVAVIGGGNSAGEESLFLTRFADRVTILVRGEAMAASQIVADKV
                     VSNPKIEVRYNTEVQALLGESKLKGIVIRNRKSGASEEINPAGAFVFIGLSPNSGWLP
                     AEIERDRYGFVVTRPNLETRLPGVFAAGDVRLGSTKQAASAVGEGATAALMVREYLKT
                     V"
     gene            6513..7613
                     /locus_tag="AC162G_15025"
     CDS             6513..7613
                     /locus_tag="AC162G_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077165.1"
                     /translation="MMSADPAYVQRLLAQAREEGQWYAGQADAAALCYEVLALFPDHR
                     EAADLVYELFCDEWTIYDTRNALQQHIDEWDDRPWQQRRRLALSYRFMSRWEGWRREY
                     EKGYERERTGPPDVADLLERGRGQLLHAYCLGDEECANFAWPLFAEAFRRTNDPRHAL
                     LWVGKQYADLGFFADAVEVLAELCSRFNDAGARRLLAEVRWWRDNAHRLPWLPPPGDG
                     SRYRRMMAFIDPDAPTDEEVIRSLRAEADRVDKVDWRPTIDPALADLVATALPPSTHE
                     TAAAATLVDWSFLDTDDGQPGELPEWANKMMRFLPRHADDIARRHRWSRPIKPPAMPP
                     RHDPDEPPFDPDELLDFDEETDEDDGDFADAL"
     gene            complement(7692..9059)
                     /locus_tag="AC162G_15030"
     CDS             complement(7692..9059)
                     /locus_tag="AC162G_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077166.1"
                     /translation="MNIKAARRFVLPIGIGLAVAWLAVAGLSTARAQSIASQAPLAVA
                     AIGRPQDPVVVTGASFPAFSGAPLGDLVLFAYRSGTWAPIPFQIDEVNITGTYVISDG
                     GLLDANDELVFMAGDAGDSVSTAIWPPDTQSRLYSRYAITVTDPLSAGGQAWVYFYRS
                     TTLIPSSARYITWTESVQTATAISYTAAFSPAAFVGLSDLFINGDSVDILDRQKTRAT
                     ALSGVVSINEETIVDLLGPVTITLPAVGPVRAATNAGGLNAALYGSRIEFDASLSGTI
                     EVPVFGSVPLDFIRTSFDWISPTISGITTYYDSNAAGGVTIDGVMDAIPTTPPNDWFQ
                     VNGGASGPGGIVAAIPNVDTSGGILSNYYRDDSTCDPGDTGDHRCYGDAGLRIDGPGP
                     VISFTLVAYVLPPGTTANVGEIYFERADNPLTAAVAEQCFAPSSSGCLRIYLPLVVKN
                     LAGAP"
     gene            complement(9089..10330)
                     /locus_tag="AC162G_15035"
     CDS             complement(9089..10330)
                     /locus_tag="AC162G_15035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5077167.1"
                     /translation="MTIRIARLTIVVLAPVALAVAMGSLLAHPAAATSTSSVCEPDGI
                     QPGGAIYRICMPPPGSWNGDLVIYAHGYVPYNVEPLAIPENQLRLPNGTSLPELVNSL
                     GFAFAATSYRTNGLAVKDGVEDLVELVDVFIYTATHGVPNHIYLTGVSEGGLITTLAI
                     EQRPDVFDGGLAACGPIGDFRKQIDYFGDFRVLFDYFFPGLLQGDPISVPQSLIDNWP
                     TYYATTIRPAILDPANADKVDQLLRVSNAPYVSGVTTTIETSIYDALSYGVLATNDGI
                     VKLGGQPFDNQDRVYSGSNDDTHLNLTVRRFSADQAALDEIEAHYQTTGQLTVPLVTL
                     HTTLDQQVPYWHETLYRVKVILNDSVALHRHGEIDRYGHCHFEAAEVLGAFLQLVSMV
                     QDPPEPFPSPRQFLPMMAAGP"
     gene            complement(10453..11142)
                     /locus_tag="AC162G_15040"
     CDS             complement(10453..11142)
                     /locus_tag="AC162G_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077168.1"
                     /translation="MRRWLIVVLCAAAMAWMESATVVYLRTLAGRVDPYQAEPLPIPV
                     DLSQTELVREAATLVMLLAVGGLAGRTWRSRLAYSLIAFGAWDMLYYAFLAVIVGWPR
                     SLLDWDVLFLIPLPWWGPVLSPMLIAAMMIVGGTLVALHDEPHRPVWPGRRAWALNLI
                     GVLLALYVFMADTIRALPGGIDATRAVLPTWFDWPLFIVALILMAAPIVDVGSKIRRR
                     NLAKALEPSRG"
     gene            complement(11426..11569)
                     /locus_tag="AC162G_15045"
     CDS             complement(11426..11569)
                     /locus_tag="AC162G_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077169.1"
                     /translation="MDIFLWLLIAILVLVPGKAVERAAGFDTVRSLALLLGVLYLITQ
                     ASD"
     gene            11768..12505
                     /locus_tag="AC162G_15050"
     CDS             11768..12505
                     /locus_tag="AC162G_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5077170.1"
                     /translation="MIRLLLVEDNQALRRAVKSGLEDTGQVSVMAETSTGEEAVSLAA
                     ADPPDAILMDVQLAGAMNGVQAAVAIRREIPRLPVVFYSIQDDERFYRDFLASGILTH
                     YAYVRKSNYLLPHMIVPLLRDAIAGRSFVDPEIEQRVQEVRLKDEDSPLDLLEPNERE
                     VIRLLAQGMTNEQIAARLGFRDRRAVSRTNGQVYAAWGLANTAEDEKTARARATLIYN
                     TGRMIVWDETGTPLVRDARGDWVPLYS"
     gene            12531..13001
                     /locus_tag="AC162G_15055"
     CDS             12531..13001
                     /locus_tag="AC162G_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077171.1"
                     /translation="MRLLLALFVGGLVVSGITAFPLRWEIDVLKRIFVDDPSSMAHAI
                     PGLVDWLAFVHRGLVETYTDYPFVAYGTDWLAFAHIVIAIVFLGPIRDPARNVWVIEW
                     GMIACALVIPTVLVFGPLRGIPFGWQLLDASFGVIGIVPLWLARRAVRQMAPEA"
     gene            13005..15116
                     /locus_tag="AC162G_15060"
     CDS             13005..15116
                     /locus_tag="AC162G_15060"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5077172.1"
                     /translation="MSRFEILLDFLLLISAHALSLFNAAALLWLGLTVLLTGNRGRPV
                     TIAGGAGLLLGAIFFAGHTLIIAAAIGTGVTVVWPIMWVVAVAAPYMWGLSIYYYAGD
                     PATGRWIRRILTLAAVAMALLLFVVEPVPPYYEFISAPNLKPSIAWAYVPYLFLCFTL
                     PLVALRRRALTPDRFRRARPWLIGAAAMLVLAVIAFAYTAYAIIPHIIPPDNLTLDLI
                     FEFFSADIVVTGFIALAIALLGRVVMAHNILLERVQSSQGFFERWPNVVLILFVGSFF
                     VALLFNAPIRPIYGLMATTMLAVFAYAMFNWRQHVERQAFLERLRPFVTSLHLENRLL
                     SPGAGDSWGEARQLFTALCRDALQAERACLLFDAPAPVAPGSLDAGQRPRRFDYAWRP
                     EHDAVLSLPAPNPADWSRLDADHWAWPLFDSRGPLGRLILGPKLNGAEYTSQELQVAA
                     ACAGRILDALAGEQLARVAMSLLRQRIAEVQVMSARHKRVLHDDLLPQIHLALLRLES
                     LRRKPGGGEEPLDEIAGELTQTHQRLSALVREMSTAAPTRLESEGCVAALHSALAHDF
                     RDSFDAIDWQADPVATERARRLPLFVGEVIFYAAQEVIRNAARHARGDDPGRKLRLDV
                     SVAGSSGDSSSGLRVVIGDDGVGRKNTAGYSVDAEDGDTGGTGSGLIFHSAMLAVVGG
                     TLGVSDREGGGTQVVIELPHD"
     gene            complement(15746..>15954)
                     /locus_tag="AC162G_15065"
     CDS             complement(15746..>15954)
                     /locus_tag="AC162G_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077173.1"
                     /translation="SSGAGRARPAPYRLVLLLTFRACLSDQVTGVHSPLAVSFTHLSQ
                     ATQGLFPLLIGLRTLGESPLNHFS"
BASE COUNT         2885 a         5060 c         5191 g         2818 t
ORIGIN      
        1 gtcgatcttg tcgcagcgac cgatcaacgc caccagagcc ggtgagtcct gccctcctcg
       61 tgttggcttg gtcgggtctg tgttttggcc agcgttcaaa gcggccagcg taagcgtgcg
      121 ttgttgggtg tcggcgattg cctgggcgtc ttggagcagt gcgttgtata gcacgccccg
      181 gcagtcgatg ttggtcgcgg ggtcgatggt gaggatacgc tgataggcat agcccaatcg
      241 ccagggaagc ggccaccacc accaccacca ccacggctgg tagctaatat ccttcttcag
      301 cacctcggag gcgcggacgt actcggcggt gacgtccacc ggcccgaaac gggccacatt
      361 gctgccggcc accgtttgcg ggccgaccag gatcacgacg aacccctcga cctcgacgcc
      421 gatgccgtac gtgccgatga aagtagccgc cgggctcccg gtcagcagct tggcgatgtc
      481 gtcgcagtcg atccggatcg cgtggtcggg tttcagcgtc accgacaccc acgcacccgg
      541 cctgatctcc ggcgcggttt caaggcgggc gcgaaccgct ttcttataca gggtgacggt
      601 gtacggctgc gagttgtgga ccaggacacc cgtttgttcc tcgacaagcg gcgctgccgt
      661 tccatagtag aactggcccg gctgcagcgg ttcctggcac acgaacttgg ccgcatagga
      721 gaggacaagc ggatcactct ctgccgtcga cgcctgcgca tcgggcgcgc cggcatcgac
      781 gacggctgcg gcggcaggcg gggcgttcga gacggcgtcc gggtttcgcg caagcgcgat
      841 gccgcccaat acgacgagtg cgcccagggc gaccagcacg atcgccaggg ccaatgcgag
      901 acgcaaccag cgagccttca tggttctcta cctccttggg gttgattgtg tcgaacctgc
      961 cggaccgaat gtcaatctgg aaagcacatc gggcgctttg ccatctgctc aagcacgcac
     1021 aaacatcgga ggtaagttac acagagttgt ccgcttcgat gacctgcagc ggtgagggca
     1081 gtaagaccca cccttgcgaa ggtttcagac ccttcgaccg cgctcagggc aggcccttcg
     1141 caagggtttc gcaaatgagt ataacccctg gcggggtgca aagtcaataa ttgttcaaga
     1201 tggaacatgg atgtgccctc ttcgattttt gccccttcca tgctacaatc atgctgcgat
     1261 gaacgagccg gctctgatcg cagcggcccg caagggcgac ctggacgcgt tcaatacact
     1321 ggtgctcgcg taccagcacc aggtgtacaa cctggcctac cgcatcatgg gcgacgaggc
     1381 gtcggccgcc gacgcgactc aggaagcctt catctcggcc tacaagcaca tggagtcttt
     1441 ccggggcgac tcgttcaagt cgtggatttt ccgcatcgtc acgaatgcgt gctatgacga
     1501 gctgcgccgc cgcaagcgcc ggcctgcgtc ctcgctggag gggcttgtcg gagacgagga
     1561 cctcgaaatc gagtacgaca tccccgacga gagcgaaacc ccggaaactc gcgtcgagcg
     1621 ccgcgagctg gcgcaagtga tagaattcgg catcgggacg ctgccgccgg atcagcgcat
     1681 cacgctcgtg ctgtcagaca tgcaaggcat gagttaccac gaaatcgccg ccatcaccgg
     1741 cgcgaacctg ggcacggtca aatctcgctt gagcagggcg cgcgccaagc tgcgggatta
     1801 cctggtggcg agcggggaac tttcgcccgg ctcgtcacgt cctagaacta ggcaggcctc
     1861 ggcacagcca acgccgccgg ggcctgcccc ttcgcaggct cagggcaagc tctgagcaaa
     1921 ggcgaaggga cgacggcttg agcctcaatg tgaagttgtc gaagtagtct gagaggcgtg
     1981 tggaatgggt ttgttcggca ggaagttgac cgaccaggag cgggccgagg agctattctc
     2041 gccctatatc gacgggcagg tgaccgccgg ggaaaagcag ttcctcgagc ggtatctggc
     2101 cgatcatccc gaggcgcatg agaagttcga gctgcttcag gcggcggttc agatgacgaa
     2161 gacgctgccg ccggtcaaag cgccgcgctc gttcgtgctg ccccgctcga tggcgcgcaa
     2221 gccgagcctg gccgtgcgca tgtatccggt cctgcgcttt gcgacggtcg cggcgatggc
     2281 gctgttcgcg ttcgcgctca tcggcgacct gacgacgtcg tctcggctgc agctcgccgc
     2341 gcccgcgcgg gagatggtgc tcagcgagag atcgcgcgag ctaacagagc cggcggcttc
     2401 ggcgccggcc gcacaactct tcgccgccac gcctgcggcc atgccgaccg cgacgcctgc
     2461 gccgcaagcg acgccggcgc cgggagcggt aggcgctcct gccaatgtcg acgcgacaac
     2521 agccgcggag gagccggccc ccccttccga gcaggccacg gctatcgccg attcgagggg
     2581 ggcacacctg gaacaaccca ccccgaccgt ggaggcgagc gcagatgcgt cggcgatgaa
     2641 ggcggaagaa cccgagtcga tttcgatcac agagcagccg cctcctgtac aggtcgacgc
     2701 gctgcgcctg gccgtgctcg cgctggccgg attggccgcc gtcctgggag taacgacgct
     2761 gatcattcga cggcgcgtcc gatgaacgag atttgcaaag gcccggtctc cgatggggac
     2821 cgggtctgtt tgttgtcaaa cgatatacac tcacggacgc gcccatgtcc cgatcgtcgc
     2881 tccctgtgaa ctactgcctc cgctgcggcc acgcgctcca agaccggctc gttttcggta
     2941 ggacgcgccg cgcctgccct gagtgtagct tcgtgttctt tcaagagccg aaggttgccg
     3001 ccggcgcgtt gatcgagcga gacggggcag tgctcctggt gcgccgctcg gtcgatccga
     3061 gacgcggcgc gtgggcgctg cccgccggct acatggagat cgacgaaggg ccggtcgcgg
     3121 cgaccatccg tgagtgcttc gaggagacgg gcctggtcgt ccgcgtcacg ggactgttcg
     3181 gcgtgtatca cattgcgaac gatccacgcg gcgcgggcgt gctgatcctg taccgggcat
     3241 cggccgaatc cggcgagctg cggcccggcg acgacgcttc ggaggtggca tacttcggcc
     3301 cggacgagct gccgcgcgac atcgcctttg cctcgacgcg gcgcgtgctg gcgcggtggt
     3361 gcgcggagag aaggatgagg gatgaggaat gaggaatgaa gaatgaggaa tgaagatatt
     3421 acccatgccc ctgggatgac tcggaaaggc atccgggggc accgtggtac aatccggcgc
     3481 gccgatagga gagtctcaac ctgagctttc gatgactatg aacgagcaga cgacaaggcc
     3541 aagccgggct gttcgacggc cggcgccgtc agcgcgaccg gcccgcacgc cgttctcttt
     3601 gatcgcgctg agcgggctgc tcggcgtggt ggtcctggca tgcgcgatgt gcagcgttat
     3661 gctcgccgcg ttggggtccg atcgcgtgtt ggcgctgcgc gatcaggtgc tgggaagaac
     3721 gcgtgaagag cgcatcgctt cactgcagca gattgtcgac gtcgtcgtgc gcggcgagga
     3781 cgcctggccg atccccggca ggggccggct gacgatcctc ctgatgggcg tcgatcgccg
     3841 cccgagcgaa ggccgcgccc cggcccgcac cgacgcgatc acgctgctca cggtcgatcc
     3901 ggaagccaag accgccgcga tgctgtcgat cccgcgcgat ctgtacgtgc cgctggccgg
     3961 cctggaccgc gtcgatcgga tcaatgcggc gtacgtgtac ggcgaagcgc gcggggtgcc
     4021 gggcggcggc gcgcaggcaa ccgaggacac gatcgcgtgg aacctgggca tcccgatcga
     4081 caaatacgcc gtcgtcgact tcgccggctt caagcaggcg atcgacgcgc tgggcggcat
     4141 cgacgtggac gtgccgaggc gcatcgtcga cgacacctac ccgacggagg atttcggcat
     4201 cgaactcctg gtgatcgagc ctggcccgac gcacatggac ggcgagctgg cgctcaagta
     4261 cgtgcgcacg cgccacgcgg atagcgactt cgggcggctg cagcgccagc agcaggtgat
     4321 gctggccgcg cgggacaagg cgctcagcct cggcgcgctc acacaactgc ctgcactgct
     4381 cgacgccgca gccgggtcgt acgagaccga cctgaccgtc gccgagatcg caagcgtggc
     4441 gaaggtcttg agcgacgttc cgcgcgagaa cattgcggcg tttcgcattg acgaaacaat
     4501 ggcgcagctt tgggcgacgc cgacgggcgc cagcgtcctg attccgctgc gcgacagaat
     4561 cgcgccggtc gtggctgcct tcctcgctca cgggcaggcg caatcgtccg cagccccttg
     4621 agccgacgcg cgaccgacgt gcgacgcact tccaaccccc tccgctacgc tacgcgggcg
     4681 agagtgcgtc gcacgtcaag aatgtgtcgt tttttttgaa acggggttga gaatgtctaa
     4741 cccctgcaat tcacacaggc gctcgatgcg catgggcggc gcacggagga tgtgactcag
     4801 gactgatgtc acgttgacca caggggcgag tgggtttaca gtgagagcac ggacggaaag
     4861 ggtcagcctg aactgtacgt caggtcggtc ctctccgttc tgcttttatt gcagcatcac
     4921 acaccccggt ggtctccctc ttcagtatgg gtcggcaagt tggccttgtc tgtcaccgcc
     4981 gagagcgcgg aagaaacctg gtttcttaga gaaaccaggt ttctgattgt gtaacaactg
     5041 cccgcaagcc gagactagac aatatctact gccagccgtt ccacgtgata ttcagcgtcg
     5101 cctcactcca gtgagtggac gcgatggcct aatcggaaaa taggttcatg cgagaggaaa
     5161 agaaatgtcc cacacccaac tcaccgtcta cggcgcgacg tggtgcagcg actgcaaacg
     5221 cgcgaagaag ttcctgggcg agcagcgcgt ccactataac tgggttgaca tcgagcaaga
     5281 cgccggcggc ctggcgctcg tcgagcgcgt caacgacggc aagcgcatca ttcccacgat
     5341 catcttcgaa gacggcggca tgctggtcga gccaagcaac gccgagctgg ccgccagact
     5401 cggcctgcag acgacggcga gcatgagcta ctacgacctg atcgtcgtgg gcggcgggcc
     5461 ggccggcctg acggccgcgc tgtacgccgc gcgagagggc ctcgacgtgc tcgtcataga
     5521 gaagagcggc ctgggcggcc aggcgggcgt gaccgagcgg ctcgacaact ttcccggctt
     5581 ccccgacggc atcaacggcg gggacttcgc cgaccggctc gcacagcagg cgcggcgctt
     5641 cggcgtcgag atcctgcagg cgcaggacgt cgtcggtttg cgcgccgagg aggaatcgcg
     5701 ctacgtgcag acggcggacg gcaacgagta tggcgcgcgg gcggtgttga tcgcgaccgg
     5761 atcgacctat cgccgcctgg gcgtgcccgg cgaggacgag ctgatcggtg cgggcattca
     5821 cttctgcgcg acatgcgacg gcccgttcta caaagggcag cacgtcgcgg tgatcggcgg
     5881 cggcaacagc gcgggcgagg agagcctgtt cctgacccgc ttcgccgatc gagtcacgat
     5941 cctggtgcgc ggcgaggcga tggccgccag ccagatcgtc gccgacaagg tcgtatcgaa
     6001 ccccaagatc gaggtgcgtt acaacaccga agtgcaggcg ctcctgggcg agagcaagct
     6061 gaagggcatc gtcattcgga acaggaagag cggcgcgagc gaagagatca acccggccgg
     6121 cgcgttcgtc ttcatcggcc tgtcgccgaa cagcggctgg ctgcctgccg agatcgagcg
     6181 cgaccggtac ggtttcgtcg tcacgcggcc gaacctggaa acgagactgc ccggcgtgtt
     6241 cgccgctggc gacgtgcgcc tgggcagcac caagcaggcg gcgagcgcgg tgggcgaagg
     6301 cgcaacggcg gcgctgatgg tgcgggaata tctcaagacg gtgtagtgac cctccccagc
     6361 tcctccccat gtcggggagg ggagatgtgc actctccctc ttgacatcgt caccgctcag
     6421 caataccata catgaccagg agcgaccctt gcgaaggctc caaagccttc gcaagggtta
     6481 caacatgatc ggaccggagg aggtacttga cgatgatgag cgccgatccc gcctacgtcc
     6541 agcgcctgct tgcgcaagcc cgcgaagaag gccagtggta tgccggtcag gccgacgccg
     6601 ctgcgctgtg ctacgaggtc ctcgccctct ttcccgacca tcgcgaagcg gccgatctgg
     6661 tgtacgagct tttctgcgac gaatggacga tctacgacac gcggaacgcg ctccagcagc
     6721 atatcgacga gtgggatgat cgtccctggc agcagcggcg ccggctggcg ctgtcctacc
     6781 gtttcatgag ccgttgggag ggctggcggc gcgagtacga gaaaggctac gagcgcgagc
     6841 gcacaggccc gccggacgtg gcggatctac tggagagggg cagaggccaa ctgctgcacg
     6901 cttactgcct gggcgacgag gaatgcgcaa atttcgcctg gccgctgttc gccgaggctt
     6961 tccgccgcac gaatgacccg cgccatgcgc ttttgtgggt cggcaagcag tacgccgatc
     7021 tgggtttctt cgccgacgcg gtcgaggtgc tggccgagtt gtgctcgcgc ttcaacgacg
     7081 caggcgcgcg ccgcctgctg gccgaagtgc gctggtggcg cgataacgcg caccgcctgc
     7141 cgtggctgcc gccgcctggc gacggctcgc gctacaggcg catgatggcg ttcatcgacc
     7201 cggacgcgcc caccgacgag gaggtcatcc gatcgttaag ggcagaagcg gatcgagttg
     7261 acaaggtcga ctggcgcccg acgatcgatc cggcgctggc cgatctcgtg gcgaccgcgc
     7321 tacctccctc gacccatgaa actgccgctg cggcaacgct cgtggattgg agtttcctcg
     7381 atacagacga tggccagccg ggcgagctgc ccgagtgggc gaacaaaatg atgcggtttc
     7441 tgcctcgcca cgccgacgac attgcccgcc gccatcgttg gagccgcccg atcaagcctc
     7501 cggcgatgcc gcctcgccac gatcccgacg agccgccttt cgatccggac gagctcctgg
     7561 atttcgacga agagaccgac gaggacgatg gcgatttcgc tgacgcgctc tgagacctcc
     7621 gaggtcttta cccgaggttc ccgccgatgg atggcatcaa agcacccgcg tcgcgcggga
     7681 agcgccggag gtcacggggc accggcaagg tttttcacca cgagcggcag atagatcctc
     7741 aaacagcccg atgatgacgg cgcgaagcac tgttcggcta cagcggccgt taggggatta
     7801 tcggccctct cgaagtagat ttcgccgaca ttggctgtcg tgccaggtgg cagaacgtac
     7861 gccacgagcg tgaaactgat gaccgggcca gggccgtcga tgcgcagtcc ggcatcgccg
     7921 taacatcggt ggtcgcccgt gtcgcccgga tcgcacgtac tatcatctct gtagtagttg
     7981 gacaggatgc cgccgctcgt atcgacattc ggtatcgctg ccactatgcc acccggcccg
     8041 ctcgcgcccc cattgacttg aaaccaatca ttaggcggag tcgtcggaat agcatccatt
     8101 acaccgtcaa tggtcacgcc gcccgctgca ttactgtcat agtaggtggt gatgccgctt
     8161 atggtcggac tgatccagtc gaaagaggtg cgaatgaagt cgagcggtac ggagccgaaa
     8221 acgggcactt cgattgttcc actcaaagat gcgtcgaact caatacgcga tccgtaaagg
     8281 gcggcgttca gaccgccagc gttggtcgct gcccgcaccg gcccgacggc aggcaaggta
     8341 attgtgacag gcccgagcaa gtccacgatg gtttcctcat tgatgctaac gacgccagac
     8401 aacgccgtcg cgcgagtctt ttgacgatcc agaatgtcca cgctgtcgcc attgatgaac
     8461 aggtcggaca ggccgacgaa agcagccggg ctgaaagcgg cagtgtatga gatggcggtc
     8521 gccgtttgca cggactcggt ccaggtgatg tagcgcgcac tgctcggaat cagcgtcgtc
     8581 gagcgataga aatagaccca ggcctgaccc ccggcgctca acggatcggt cacggtgatc
     8641 gcgtaacgtg agtaaagacg cgactgagta tcggggggcc agatcgccgt gctcacgcta
     8701 tcgcccgcgt cgcccgccat gaagaccagc tcgtcgttgg catccagcag gccaccatcg
     8761 ctgatgacgt aagtgccggt gatgttgact tcgtcgatct ggaaggggat gggcgcccag
     8821 gtcccggagc ggtaagcgaa caagaccagg tcgcccaggg gcgcgcctga aaaggcaggg
     8881 aagctcgcgc ctgtcacgac gaccgggtct tggggacgtc ctatcgcagc gacggccaga
     8941 ggcgcttgcg atgctatcga ttgcgcccgg gctgtgctca agcccgcgac ggccagccat
     9001 gcgacagcca gcccgattcc tatcggcagc acaaatcgtc gagcggcttt gatgttcatg
     9061 gcttcatcct ccccttttca aatggctgtc aaggcccagc cgccatcatc ggcaggaatt
     9121 gcctggggct ggggaatggc tccggtggat cttgcaccat actcactaac tgcaaaaagg
     9181 ctcccaggac ctcggccgcc tcgaagtgac agtgaccata tcggtcaatc tcgccgtgcc
     9241 gatgcagcgc gaccgaatcg ttcagaatga ctttcacgcg gtagagcgtc tcgtgccagt
     9301 aaggcacttg ttggtcgagc gtggtgtgaa gggtgaccag gggaacggtg agctggccgg
     9361 tcgtctggta gtgggcttcg atttcgtcca gcgccgcctg gtcggcgctg aagcgccgca
     9421 ccgtgagatt gaggtgcgtg tcgtcgttcg agccactgta gactcgatcc tggttgtcaa
     9481 acggctggcc gcccagcttg acgatcccgt cgttggtggc caggacaccg tacgagagcg
     9541 cgtcgtatat cgaggtctca atcgtggtgg tcacgccgga aacgtagggc gcgttgctca
     9601 cgcgcaggag ttgatcgacc ttgtctgcgt tcgccggatc caaaatcgcg ggccggatag
     9661 ttgtcgcgta gtaggtgggc cagttgtcga tgagcgattg ggggacgctg atgggatcgc
     9721 cctgcaacag gccaggaaag aagtaatcga acagaacccg gaagtcgccg aagtaatcga
     9781 tctgcttgcg aaagtcgccg atcggcccgc aggcggccag cccgccgtcg aagacgtccg
     9841 ggcgctgctc gatcgccaga gtcgtgatca agccgccctc ggacacgccg gtcaaataga
     9901 tgtggttcgg tacaccgtgc gtcgccgtgt agatgaagac gtcgaccagc tcgaccaggt
     9961 cttcgacgcc gtctttgacg gccaggccgt tcgtgcggta actggtggcg gcgaaggcga
    10021 accccagcga attgacaagc tcgggaagtg aagtgccatt ggggagcctg agctgattct
    10081 cgggtatcgc cagcggttct acgttgtaag gcacgtagcc gtgcgcgtag atcaccaggt
    10141 cgccgttcca ggatccgggc ggcggcatgc agatgcgata gatcgcgccg ccaggctgta
    10201 tcccgtccgg ctcgcatacg ctcgacgtgc ttgttgccgc tgccggatgc gcgagcaggc
    10261 tgcccatcgc gactgccaac gccaccggcg cgagaacaac aatggtcaac cgagcaatgc
    10321 gtatcgtcaa ttggccgtcc taactgcgcc tttcacatcc ggcgcgggca tccgggatcg
    10381 atatagagct gtctactggg agcgtgccca actataacac tgccctgcaa gaatggtcaa
    10441 ccccccgcac cctcaacctc gcgaaggctc caaagccttc gcaaggtttc ttctgcgaat
    10501 cttgctcccc acgtccacga tgggcgcagc catcaggatc aacgctacga tgaacagcgg
    10561 ccagtcgaac catgtgggca acacagcgcg ggtggcgtcg atgccgccgg gcaacgcgcg
    10621 gatcgtgtcg gccatgaaga cgtacaacgc cagcaggacg ccgatcaggt tcagcgccca
    10681 cgcccgtcga ccgggccata ccggtcgatg gggttcatcg tgcagcgcga ccagcgtccc
    10741 gccgacgatc atcatcgccg cgatgagcat cggggagagc accggccccc accacggcag
    10801 cgggatcagg aacagcacat cccaatcgag cagcgaacgc ggccagccga cgatcaccgc
    10861 caggaacgcg taatatagca tgtcccatgc gccgaacgcg atcaacgaat aagccagccg
    10921 gctgcgccac gtccggccgg ccagcccgcc gacggcgagc agcatgacca gcgtcgccgc
    10981 ctcgcgcacg agctcggtct gactgaggtc gaccggaatg ggcagcggct cggcctggta
    11041 cggatcgact cggccggcca gcgtccgtaa atagacgacg gtggccgatt ccatccatgc
    11101 catcgccgcc gcacacagca cgacgatcaa ccagcgtctc atttcgctca caggtcgctc
    11161 ctcttctcac cggggaaagg gtatgggata cggaagccat ctcctcgggc ggatgacggt
    11221 tgtcggggag caggtgctga tcagaaatca tatgccgtag tatagcagat gccgccgctg
    11281 tgctggcgcg gtggggtata aaaagatacc tcaagttcca ccttgacaac atttatgtcg
    11341 caggtggtgg gattgccgag attcaacttc ccaatgcaag tcgggttctg tgacctctgt
    11401 gctgattatt atcttcatca cttggtcagt ctgaagcctg ggtgatcagg tatagcactc
    11461 ccagcagcag cgccaggctg cgcaccgtgt cgaagccggc ggcgcgctcc accgccttcc
    11521 ccggaaccag gaccaggatc gcgatcaaca gccataagaa aatgtccatg ccgcacctcc
    11581 agcgagatcg ggcgaacacg ctaaccgact ccaatgtaac ctgactcgcg ctctccacga
    11641 aggccctgcg tcatgctcag cctgcactca cgtgcttgac agccactcat tgcgaccgga
    11701 taatatcttt gttgtctgaa tcccgtctat taaacgtggc atccgcgcaa attcgtgcac
    11761 agtcgccatg attcgtttgc tcctcgtcga agataaccag gcgctgcgcc gcgcggtgaa
    11821 gtcgggcctg gaagacaccg gccaggtgtc ggtgatggcc gaaacgagca cgggcgaaga
    11881 ggccgtatcg ctggcggcgg ccgacccgcc cgacgcgatc ctcatggacg tccagctcgc
    11941 cggagcgatg aacggcgtgc aggccgccgt cgccatccgg cgcgagatcc cgcgcctgcc
    12001 ggtcgtcttc tactcgattc aagacgacga gcgcttttac cgcgatttcc tcgcctcggg
    12061 catcctcacc cactacgcct acgtccgcaa gagcaactac ctgctgccac acatgatcgt
    12121 gccgctgctg cgcgatgcga tcgccgggcg cagtttcgtc gatcccgaga tcgagcagcg
    12181 cgtgcaggaa gtgcgcctga aggacgaaga ctcgccgctc gacctgctcg aaccgaacga
    12241 gcgcgaggtc atccgcctgc tggcgcaggg catgacgaac gagcagatcg cggcgcgtct
    12301 cggcttccgc gatcggcgcg ccgtcagccg caccaacggc caggtttacg cggcgtgggg
    12361 attggcgaac acggccgagg acgagaagac cgcccgcgcg agggccacgt taatctacaa
    12421 taccggccgg atgatcgtgt gggacgagac cggcacgccg ctcgtgcgtg acgcgcgcgg
    12481 cgactgggtg ccgctctaca gttgacatga atcctcgtca gcttacgcgc gtgagactcc
    12541 tcctcgccct cttcgtcggt gggctggtcg taagcgggat tactgcgttc ccactgcggt
    12601 gggaaatcga cgtcctcaag cgcattttcg tcgacgaccc gtcgagcatg gcgcacgcta
    12661 tccccggact cgtcgattgg cttgcctttg tgcatcgcgg cctcgtcgag acgtacacgg
    12721 actatccgtt cgtcgcctac ggaacggact ggctggcctt cgcccacatc gtcatcgcga
    12781 tcgtcttcct gggcccgatt cgcgatccgg cgcgcaacgt gtgggtcatc gagtggggca
    12841 tgatcgcctg cgcgctcgtc atcccgaccg tgctcgtctt cggcccgctg cgtggaatac
    12901 ccttcggctg gcaactgctc gacgcgtcgt ttggggttat tgggatcgtg ccgctgtggc
    12961 tcgcgcgccg ggcggttcgt cagatggccc ctgaggcttg agcggtgtcc cgcttcgaaa
    13021 tcctgctcga cttcctcctg ttgatcagcg cgcacgcgct gtcgctgttc aacgccgccg
    13081 ccttgctgtg gctcggcctg acggtgttgc tcaccggcaa tcgaggccgt cctgtgacga
    13141 tcgccggtgg agccgggctg ctgctcggcg cgatcttctt cgcggggcac accttgatca
    13201 tcgcggcggc gatcggcacc ggcgtgaccg tcgtgtggcc gatcatgtgg gtggtggccg
    13261 tcgccgcgcc gtacatgtgg ggcctgtcga tttactacta cgcgggcgac ccggcgacgg
    13321 gccgctggat tcggcgcatc ctgacgcttg cagcggtggc gatggcgctc ctcctgttcg
    13381 tcgtcgagcc ggtgccgccc tactacgaat tcatctccgc gccgaacctc aagccttcga
    13441 tagcctgggc gtacgtccct tacctgttcc tgtgcttcac cttgccgctc gtcgcactgc
    13501 gccggcgagc gctcaccccc gaccgcttcc gccgcgcccg gccctggctg atcggcgcgg
    13561 cggcgatgct cgtgctggcc gtgattgcct tcgcctacac ggcgtatgct atcatcccgc
    13621 acattattcc accggacaac ctgacgctcg atttgatatt cgagttcttc tcagccgaca
    13681 tcgtcgtgac cggcttcatc gccctggcca tcgccttgct cggccgcgtc gtcatggcgc
    13741 acaacatcct gctcgagcgc gtgcagtcga gccagggctt cttcgagcgc tggccgaacg
    13801 tcgtgctcat cctgttcgtc ggctcgttct tcgtcgccct tctgttcaac gcgccgatcc
    13861 gcccgatcta cggcttgatg gcgacgacga tgctcgccgt attcgcctac gccatgttca
    13921 actggcggca gcacgtcgag cggcaggcgt tcctggagcg gctgcgccct ttcgtgacca
    13981 gcctgcacct ggagaatcgg ctgctgtcgc ctggcgccgg cgacagttgg ggcgaagcgc
    14041 gccagctttt caccgcgctg tgccgcgacg cgctgcaggc cgagcgcgcc tgcctgcttt
    14101 tcgatgcgcc ggcgccggtc gcgcccggtt ctctcgacgc cgggcagcgc ccgcgccgct
    14161 tcgattacgc gtggcggccc gagcacgacg ccgttctgtc gctgccggcg cccaacccgg
    14221 cagactggtc acggctcgac gccgaccact gggcgtggcc gctcttcgat tcgcgcggcc
    14281 cgctcggacg cctgatcctc ggtcccaaat tgaacggcgc ggaatatacc tcgcaggaac
    14341 tgcaggtggc ggcggcctgc gccgggcgca tcctcgacgc gctcgccggc gagcaactgg
    14401 cgcgcgtcgc gatgagcctg ctgcggcagc gcatcgccga ggttcaggtg atgagcgcgc
    14461 ggcacaagcg cgtcctgcac gacgatcttc tgccgcagat ccatctcgcg ctcctgcgcc
    14521 tcgaatcgct gcggcggaag cccggcggcg gggaagagcc gctcgacgag atcgcgggcg
    14581 agctgacgca gacgcatcag cgactgtcgg cgctggtgcg cgagatgagc accgccgcgc
    14641 cgacgcggct cgaaagcgag ggctgcgtcg ccgcgctgca cagcgcgctg gcgcacgact
    14701 tccgcgacag cttcgacgcg atcgactggc aggccgatcc ggtcgcgacc gagcgggcgc
    14761 gccggctccc acttttcgtg ggcgaagtga tcttctacgc ggcgcaggaa gtgatccgca
    14821 acgcggcccg gcacgcgcgc ggcgacgatc cgggccgcaa gctgcgactc gacgtatcgg
    14881 tcgccggctc gtccggcgac agctcctccg gcttgcgggt tgtcattggc gacgacgggg
    14941 tggggcgaaa gaacacggcg ggctattcag tcgatgccga ggatggcgac acaggcggca
    15001 cgggaagcgg cctcatcttt cacagcgcga tgctcgccgt cgtcggcggc acgctcggcg
    15061 tgtcggatcg agagggtggg gggacgcagg tcgtcatcga attgccacac gattgactca
    15121 aactcccaac ccttcgactt cgctcagggc aggcttcaaa ctccaaactt caaaaatgtc
    15181 attccgaacg tagtgaggaa tctctcctcc ggccggcgcg cgtggcgcgc cggagtagag
    15241 atgcctcgtc gttcctcctc ggcatgacac gtctgccggt gtcattccga acgcagtgag
    15301 gaatctctcc tccggccggc gagcgtggcg cgccggagta gagatgcctc gtcgttcctc
    15361 ctcgacatga cacgtctgcc ggtgtcattc cgaacgtagt gaggaatctc tcgtctgacc
    15421 ggcgcgcggc gtgccggggt cgagatgctt ggcgccgatc gtcggcggca cgccagtttc
    15481 tgccgtaccg gatggcgcgt tttgccatgg gggactcaca atgacggctg agtatttaca
    15541 cctctacgag cgcaaattgg ggagatggcc gaagactgga ggttgcccaa cgcgcggata
    15601 ttgacggtgt gtatataagt gtgcataatc tcgtttggag gcgaacatgg cagctttaga
    15661 aagagtgcag gtactcttga agcctgcgca gcgtcgcgcg ttggccagga ttgccaggcg
    15721 cgagggacgc agcatatccg aggtcttacg agaaatgatt gagtggggac tcacccagcg
    15781 tgcgcagacc aatcaacagt ggaaacaagc cctgggtcgc ctgggacaga tgcgtgaagc
    15841 taaccgcgag cggggagtgt accccggtga cctggtcgct gaggcacgcg cggaacgtga
    15901 gcaacagaac gaggcgatat ggcgcaggtc gcgcgcgacc ggccccggat gatc
//
LOCUS       DBNHXB010000370        11151 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_272075, whole genome shotgun sequence.
ACCESSION   DBNHXB010000370 DBNHXB010000000
VERSION     DBNHXB010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11151
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_272075"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="AC162G_15070"
     CDS             complement(<1..350)
                     /locus_tag="AC162G_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR5077174.1"
                     /translation="MEKTLVNPASLAKPSGYSNGVRVGGGRLLFLAGQTGMDATGAIA
                     HPGDVVAQFRQALANLKTVVEEAGGTMTDIVKLTLFVTDKAAYRLNLKPIGEVYRSVF
                     GRHYPAMTLVEVKSL"
     gene            complement(386..1177)
                     /locus_tag="AC162G_15075"
     CDS             complement(386..1177)
                     /locus_tag="AC162G_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HIR5077175.1"
                     /translation="MTYDFLYTVENSIAMITLNRPEVLNAITLEIYAQLRDLFEDLRT
                     DEAVKAVVITGAGRAFCSGGDVHKIIGAVIDRDMRGHLEFCRMTGHLVRNMRLLAKPI
                     VAAVNGLAAGAGAVITLACDLRIVAEDAKFAFLFTRVGLTGADMGAAFLLPRIVGQGR
                     AMELLMLGDDVDAHTAERIGLANRVMPKEQVLPAAREWAERLASGPTLSIGMTKIMVN
                     NEWNMDVVSAVEAEAQAQALMMMGQDHRAFYEAFKEKRKPEFRGR"
     gene            1398..2156
                     /locus_tag="AC162G_15080"
     CDS             1398..2156
                     /locus_tag="AC162G_15080"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR5077176.1"
                     /translation="MLPDELEDIWRDRPVACCAWGALEWHGPHLPLGLDGLVAERFAE
                     RLVDRAGGVVLPTVWLPITALPHAFSLSTHTEVVKGIWRDLLSELYRAGARVVCLITG
                     HYAQGHEIELYQASMAAMRAYPDLLVLSATPLEILGDDDYLDHAGQWETAQLMAVRPD
                     LVHLEKFPGMLGAKQVAVLGTDPRAATREDGEAVTLRALDAWAGWIDRLLKERDQQPL
                     FAWYARRIADYAGYTRRYYSGSWEEAIQKWWQER"
     gene            2282..3169
                     /locus_tag="AC162G_15085"
     CDS             2282..3169
                     /locus_tag="AC162G_15085"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HIR5077177.1"
                     /translation="MSSLREAMTRFSPVLRRLGGVIVLGIIPLAVGIGLAQLVAPQPA
                     VGIVKINAEIWPLSLNFFMQEIEEARRDPHIKAVVIQVDSPGGEVVATQSLYLEIQKL
                     RSQMPVVASIDSTAASGAYYVAVAADSLYAKPSSIVGNIGVWGIVPPDIGVNDAVLAS
                     GPFKLTASNQEEFIRRIEGIKQEFIATVVSQRGERLKISPVDLAQGLAYAGREAQHLG
                     LIDHLGTQADAVAAVAAQAGIVNYDVVDLEARVVARLLAGTPTPPASWSGAADPQTGR
                     RQMPPGVYLLYDAQLGGAP"
     gene            3166..5388
                     /locus_tag="AC162G_15090"
     CDS             3166..5388
                     /locus_tag="AC162G_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077178.1"
                     /translation="MTRGRQILLVVLLIALPVALQSLWFYRGRYTPPSVPGLDEVQIT
                     LATPEYRFVPGEPKESTARVVIDLSHANNLEVDDLSPLRDRLAALGATVLTFEGPSDA
                     LAAELRRASAWIAIAPTTAYSPEQRDTIAAFVADGGRLLIAADPTRSVPPLEEDAFDP
                     FGLFSRISAVPAANSLAGAFGIVYFDDYLYNLVENEGNYRNVKFNEFGDHALTKDLRT
                     VTLFATHSLRTNGRALVVGDADTRSPLRSGESDLAAAVLGAGERVLALGDVSLFSAPY
                     HTLADNDRFLSNIANWLAEDKRDWSIAEFPYLFKGPVDLVQANGGNLDPRLIARSAEL
                     QAAFQKAGLPLSLRAEAAPDRDTLFVGTFDDLEAVQEFLVTAGVTVTLPAEDETDSGE
                     PAPLETGSASSTPESVETGTPTSTPFAEEEEAASQAEPFATRQVSGTITIEGIGVVPV
                     AGTTLYVSDDNGGRAVVVTLAEDAEGAIKALDRLESNDFGNCVHGGAVTVCSTGEGEE
                     GSGLGSAGAQAPGDAQKPGEGQRSRILILVRDIGRTGARTSEQELESMLTEAAYSVKV
                     WSMRRDGTPASSDTTGYDAIIVDSGDYAFDSGDIESLATLTSLRGRVLFIGAQPVPVP
                     EGETALISDLVVVDAGHPIAKGFEQDQVIPLNPSESGVPALVIQPEALDSAGAEVAVI
                     FERGPESEEAGARALVAGTGGVGDTVERAIVAAFALYQLPEDMQRMLVLNAVGWLLAE
                     "
     gene            complement(5505..6773)
                     /gene="kynU"
                     /locus_tag="AC162G_15095"
     CDS             complement(5505..6773)
                     /gene="kynU"
                     /locus_tag="AC162G_15095"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HIR5077179.1"
                     /translation="MPIRADAPYAHELDRQDELARFHDEFVIGDPDLIYLDGNSLGRM
                     PRRSAARIHDVVEREWGRRLIRGWGEGWFTAPQRIGSKVAQLVGARPDEVIVGDSTTV
                     NFFKLVMAALRARPGRVKIVTDDLNFPSDVYVLQSALNLAGPGHRLEIVRSGDGISVS
                     NEALAEAIDDRTALVTLSHTLFKSGYVYDMPLVTDMAHRADALVLWDLSHSVGAMPIT
                     LDEAGVDLAVGCTYKYVNGGPGAPAFLYVRSDLQESLLNPIWGWFGQKGQFDLKLDYE
                     PNPGIARFLVGTPAMLSLAAIEPGIDLLLEAGLDRLRAKSVKQTEYLIGLWETLLQPL
                     GFSLNSPRDPERRGSHVSIGHPDALRIDRALIEEMNVLPDFRYPDNIRLGIAPIYTSF
                     AEIHEAVTRICRVMSERLYEKYSTERPEVT"
     gene            complement(6856..7824)
                     /locus_tag="AC162G_15100"
     CDS             complement(6856..7824)
                     /locus_tag="AC162G_15100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR5077180.1"
                     /translation="MTRILSLDDDPDVVSLIGLILKRAGYEHLGTRDSHEAWAMLHTH
                     PIDLFTQDLMRPDMDGWKFYEQMKSDEALRDIPVIILTAKAQSVDKDLGLQAFKVDGY
                     IAKPFGPQELLAAIREALTKRGKPLPSEVEYAHRKSLPRKSVRERIAALHLDLSLFLP
                     FQKRRKSVQERIAALQDVDPEVRLSAVRLLGEIGDKRAAEPFIEMLEDEHGEIRLAIV
                     TALGMLGDTRAVEPLIRVLREEAWTIRWSAAMALGRIKNARAVEPLIAALSDQSSIVR
                     MIAALALGEIKDERAVEPLAQALADEDNWVRWTVAKALRQIKGARS"
     gene            8315..9388
                     /locus_tag="AC162G_15105"
     CDS             8315..9388
                     /locus_tag="AC162G_15105"
                     /inference="COORDINATES: protein motif:HMM:NF021599.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HIR5077181.1"
                     /translation="MAAPPQPADLEALSRRFHRFATLETGGSSPLYERLCLGISQDAD
                     VLALAAQARSHPVPNILLAAVHYLLLKGVQHPLAAFYPSVSNETHPGGDPYPHFRAFC
                     IEHRDEILNLIRTRLVQTNEVHRCAYLALAFGIVAERAGHRPLALIDVGTSAGLNLLW
                     DRFGYDYGRRRLGDPGAPVQLTCELRGVRRPPIPERLPAVASRAGIDLSPVDVRDPDA
                     VLWFRALIWPEHRERVELLMRAIQLARQDPPELIAGDALELLPQVIAAVPRGTPVCVF
                     HTHTANQFPREAREQLIALIEEHGRRRDVYRVSAEWIGTPQTQLELVAYEQGVKTEKL
                     LAYVDGHARWIEWLDKRLPTSGT"
     gene            9485..10084
                     /locus_tag="AC162G_15110"
     CDS             9485..10084
                     /locus_tag="AC162G_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5077182.1"
                     /translation="MEERPAIARLKQGDIGGLETLVRIYQVRAVRAAYLVVRDRALAE
                     DIVQAAFVRAYERIGQFDANRPFGPWFLKSVINDSLKAITQQQRNVSLDWEAEREDTT
                     WADRLIDPAPGPLEMAESADMQRMVQEALGQLTPAQRTAVVLRYFLDFSEAEMTTKLN
                     RPPGTVRWLLYAARERLRDILSSLLPDSGSHGSQVSKND"
     gene            10100..10885
                     /locus_tag="AC162G_15115"
     CDS             10100..10885
                     /locus_tag="AC162G_15115"
                     /inference="COORDINATES: protein motif:HMM:NF025643.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HIR5077183.1"
                     /translation="MQHELIRQNLRRIAEQAVPDDTDLWPAIHARLIAPRRRGHSTVG
                     NTFRRVFAFSAITLVVFATILLSVPPARAAVLEFLRELGGWIFTDEQTSAQQVVADPS
                     LIPTVLPNDGEEVERLDVTESEERLGESIYVPSYIPTGYKLIARTGPGQNASDVAYMI
                     TSYRNSAEASDFFIVQGKVKVPQQADMGVEPTEVFVRGQTGLWFERAPISYSARYEEG
                     GDVIMELGIRNVLVWEEGDGYLSISSSNLPLEETLKVAESLTK"
BASE COUNT         2052 a         3507 c         3440 g         2152 t
ORIGIN      
        1 aggctcttca cctcgaccag cgtcatcgcc ggataatggc gaccgaagac cgatcgatat
       61 acttcgccga tgggcttgag gttcaggcga taggcggctt tgtcggtcac gaaaagggtg
      121 agcttcacga tgtccgtcat cgttccgccc gcttcttcga cgaccgtttt gagattggcg
      181 agagcctggc ggaactgggc gaccacgtcg ccgggatgcg cgattgcccc ggtcgcgtcc
      241 atgcccgtct gcccggcgag aaagagcagc cggccgccgc ccacccggac accgttcgaa
      301 tagccggatg gtttagcgag cgacgccgga ttcacgagag tcttttccat acttgaattc
      361 ccctctctaa ctccccactc taactctacc ttcccctaaa ctccggcttc ctcttctcct
      421 tgaacgcctc gtagaacgcc cggtgatcct gccccatcat catcagcgcc tgggcctgcg
      481 cttcggcttc gacggcggac accacgtcca tgttccactc gttgttgacc atgatcttgg
      541 tcatgccgat ggagagtgtc ggcccgctcg ccagccgctc ggcccattcg cgcgccgccg
      601 gaagcacctg ttccttcggc atgacgcggt tcgccagccc gatgcgctcc gccgtgtgcg
      661 cgtccacgtc gtcgccgagc atcaacagct ccatggcccg gccctgcccg acgatacgcg
      721 gcagcaggaa cgccgcgccc atgtccgccc ctgtcaggcc gacccgtgtg aacaggaagg
      781 cgaacttcgc gtcttcagcg acgatgcgca ggtcgcacgc cagcgtgatg accgcgcccg
      841 cgcccgccgc caggccgttg acggccgcga cgatcggctt ggcgagcagc cgcatgttgc
      901 gcaccaggtg gccggtcatc cggcagaact ccaggtggcc gcgcatgtcg cgatcgatca
      961 ccgcgccgat gatcttgtgc acgtcgccgc cggagcagaa cgcgcggccc gcgccggtga
     1021 tcaccaccgc cttcaccgct tcgtcggtgc gcaggtcttc gaagagatcg cgcagttgcg
     1081 cgtaaatctc cagcgtgatc gcgttcagta cttccgggcg gttgagcgtg atcatcgcta
     1141 tcgagttttc gacggtatag aggaagtcgt aagtcattgg gcttggctcc tgtgggtttt
     1201 tccagctcga agcggactgc caagtccgcg ttggcgtctg gacctggcag tccagtccga
     1261 gcaaaaagga attttgaagc aggttctaga tcatattcaa acttgggcaa aaggtcaatc
     1321 cggtgctaaa ctggtgatgg cacacaaatc gtactggcca cggagacacg gagaccaagc
     1381 ctcaccgcta cgccgaactg cttcccgacg aactcgaaga catctggcgg gatcggccgg
     1441 ttgcctgctg cgcctggggc gcgctcgaat ggcacggccc gcatttgccc ctcggcctcg
     1501 acgggctggt agccgagcgc ttcgccgagc ggctggtcga ccgcgccggc ggcgtcgttc
     1561 tcccgaccgt ctggctcccg atcacggcgc tgccgcatgc gttctcgcta tcaacgcata
     1621 ccgaagtcgt caaaggcatc tggcgcgatc tgctaagcga gctgtaccgc gccggcgcgc
     1681 gcgtcgtgtg cctcatcacc ggccactatg cgcaagggca cgagatcgag ctgtaccagg
     1741 cgtcgatggc ggccatgcgc gcttaccccg acctgctcgt gctgtccgcc acgccgctcg
     1801 agattcttgg cgacgacgac tacctggatc atgccgggca gtgggagacc gcgcaactga
     1861 tggccgtccg tcccgatctc gtgcatctgg agaaattccc cggcatgctc ggcgcgaagc
     1921 aggtcgccgt gctggggacg gacccgcgag ccgccacccg tgaagacggc gaagcggtca
     1981 cgctcagggc gctggacgcc tgggcggggt ggatcgatcg gctgttgaag gagcgcgatc
     2041 agcagccgct cttcgcctgg tacgcacggc gcatcgccga ttacgccggc tacacgcggc
     2101 gctactactc cggctcgtgg gaggaagcga ttcagaaatg gtggcaggaa aggtagactt
     2161 cggaagtctt ttgacgactt ccgaagtctg aggcccatac gcactcaggt gtatgccgcc
     2221 atcgggaaac cgtgatacac tgttgccgcg tgacacggaa tggatggggc ggagggcatc
     2281 catgtcgtct ctacgggaag ccatgacgcg attttcgccc gtgcttcgga ggctcggcgg
     2341 tgtgatcgtg ctggggatca ttccgctggc cgttggcatt gggctggccc agcttgtcgc
     2401 cccgcagccg gcggtgggca ttgtcaagat caacgccgag atctggccgc tgagcctgaa
     2461 cttcttcatg caggaaatcg aggaagcgcg gcgcgatccg cacatcaaag ctgtcgtgat
     2521 ccaggtggat tcgcccggcg gcgaggtcgt cgccacacag agcctgtacc tggagataca
     2581 gaaactgcgc agccagatgc ccgtcgtcgc ctcgatcgac agcacggcag ccagcggcgc
     2641 gtactacgtc gccgtggcgg cagactcact ttacgcgaaa ccttcgtcca tcgtcggcaa
     2701 catcggcgtg tggggaatcg tcccacccga catcggcgtc aacgacgccg tcctcgcgag
     2761 cgggccgttc aagctgacgg ccagtaacca ggaagagttc atccgccgga tcgagggcat
     2821 caagcaggag ttcatcgcca ccgtcgtgag ccagcgcggc gagcgcctga agatctcgcc
     2881 ggtcgatctc gcgcaggggc tggcttatgc gggtcgggag gcccaacacc tgggactgat
     2941 cgatcacctc ggcacgcagg ccgacgcggt tgcggcggtc gcagcgcaag ccggtatcgt
     3001 caactacgac gtcgtcgatc ttgaagcgcg cgtggtcgcc aggctgctgg ccggcacacc
     3061 aaccccaccg gcatcctggt ccggcgcagc cgatccgcag accggacgcc gccaaatgcc
     3121 ccccggtgtg tatctgctgt acgacgctca actgggaggc gcgccgtgac gcgcggccgt
     3181 cagatcctgc tcgtcgtgct cttgatcgcg ctgccggtcg cgctgcagtc gctgtggttc
     3241 tatcgcggtc gttatacccc gccgagcgtg cccggcctcg acgaggttca gattacgctg
     3301 gcgactcccg aataccgctt cgtccccggc gagccgaagg agagcacggc gcgcgtcgtg
     3361 atcgatcttt cgcacgcgaa caatctggaa gtcgacgatc tcagcccgct gcgcgatcgg
     3421 ctggcggcgc tcggcgcgac ggtgctcaca ttcgaggggc cgagcgatgc gctcgccgcc
     3481 gagctgcgcc gcgccagcgc ctggatcgcg atcgcgccga cgacggctta ttcgccggag
     3541 cagcgcgaca cgatcgccgc attcgtcgcc gacggcggcc gactgctcat cgccgccgac
     3601 ccgactcgct cggtgccgcc gctcgaggaa gacgctttcg atccgttcgg gcttttctcg
     3661 cgtatcagcg ccgttcccgc cgccaacagc ctggcaggcg cgttcggtat cgtctacttc
     3721 gacgactacc tgtacaacct ggtcgagaac gagggcaact atcgcaacgt caaattcaac
     3781 gagttcggcg accacgcgct cacgaaggat ctgcggacgg tcaccctctt cgcgacgcac
     3841 tcactccgca ccaacgggcg cgcgctcgtc gtcggcgatg ccgatacccg gtcgccgctg
     3901 cgcagcggcg agtccgacct cgccgcagcc gtgctgggcg ccggcgagcg cgtgctggcg
     3961 ctgggggatg tctcgttgtt cagcgcgcca taccacaccc tcgccgacaa cgatcggttt
     4021 ctgagcaata tcgccaactg gctggcggag gacaagcgcg attggagtat cgccgagttt
     4081 ccgtatctct tcaaaggccc ggtcgatctg gtgcaggcca acgggggtaa tctcgatccg
     4141 cgcctgattg cgcgcagcgc cgagctccag gccgctttcc agaaggctgg gcttcccctc
     4201 agcctgcgcg ccgaggctgc gcccgatcgc gatacgctct tcgtcggcac gttcgacgat
     4261 ctggaggccg tccaggaatt cctggtcact gccggcgtca cggtgacgct cccggcggag
     4321 gatgagacgg attccggcga gccggcgccg ctcgagactg gatctgcctc atcgacgcct
     4381 gaatcagtcg aaacaggcac gccaacttcg acaccgtttg ccgaagagga agaggctgcc
     4441 tcacaggcag aaccgtttgc cacccggcaa gtttccggca cgattactat cgagggcatc
     4501 ggcgtggttc ccgtcgccgg tacgacgttg tacgtcagcg acgacaacgg cggccgagcc
     4561 gtcgtcgtca ccctggccga ggacgcagaa ggggcgatca aagcgctcga tcgcctggaa
     4621 tccaacgact tcggcaactg tgtgcacggt ggcgccgtga ccgtgtgctc gacgggagaa
     4681 ggcgaggagg gatcagggct tggatcggcc ggcgcccagg cgccgggcga cgcccagaag
     4741 cctggcgaag ggcagagatc ccgcatcctg atcctcgtgc gcgacatcgg gaggacggga
     4801 gcgcgaacca gcgagcaaga gctggagagc atgctgaccg aggcggcgta ctctgtgaag
     4861 gtgtggtcga tgcggcgcga cggcacgccg gccagcagcg atacgactgg ctacgacgcc
     4921 atcatcgtgg actccggcga ctacgctttc gacagcggag acatcgagtc gctggccacg
     4981 ctcacgagtc tgcgaggccg ggtgttgttc atcggcgcac agccggttcc ggtgcccgaa
     5041 ggcgagacgg cgctgatcag cgatctggtc gtcgtcgatg ccggccatcc gatcgcgaag
     5101 gggttcgagc aggatcaggt catcccgttg aatccctccg aaagcggtgt gccggcgctg
     5161 gtgatccagc cagaagcgct tgattcggcg ggcgcagagg tcgccgtgat cttcgagcgc
     5221 ggccccgaga gcgaagaggc cggcgcgcgg gcgctcgtgg cgggcaccgg cggcgtcggt
     5281 gacacggtcg agcgtgccat cgtggcggcg tttgcgctgt accagttgcc agaagatatg
     5341 cagcgcatgc tcgtgctgaa tgcggtgggg tggctgttag cggagtaatt ggagattgga
     5401 gattggaggt tggagattgg aggttggagg ttggaggttg gtggttagag attgggagtt
     5461 tgaagtttgg agtttgaaat ttgggatttt gatcttttca ttcctcaggt aacctcgggt
     5521 ctctcggtcg aatacttctc gtacagccgc tcgctcatta cccggcagat gcgcgtgacc
     5581 gcttcgtgaa tctccgcaaa cgaggtgtag atcggcgcga tgccgagtcg gatgttgtcg
     5641 gggtagcgaa aatcaggcag cacgttcatc tcctcgatca gcgcccgatc gatgcgcagc
     5701 gcgtcgggat ggccgatcga cacgtgcgag ccgcgccgct cgggatcgcg cggggagttg
     5761 agcgagaagc cgagcggctg caatagggtc tcccagaggc cgatcagata ttctgtctgc
     5821 ttcaccgact tcgcgcgcag gcgatccagc cccgcttcca gcagcaggtc gatgcctggc
     5881 tcgatcgccg cgagcgagag catcgccggc gtgccgacga ggaaacgcgc gatgcctgga
     5941 tttggctcgt agtccagctt caaatcgaac tgcccttttt ggccgaacca gccccagata
     6001 ggattgagca ggctttcctg caaatcgctg cggacgtaaa gaaacgcagg cgcgcccggc
     6061 cccccgttga cgtacttgta cgtgcagccc acggccaggt cgacgccggc ttcgtcgagc
     6121 gtgatcggca tcgcgcccac cgagtgactc aggtcccaca acaccagcgc gtccgcgcga
     6181 tgcgccatgt cggtgacgag cggcatgtcg tatacgtagc cgcttttgaa cagcgtgtgt
     6241 gagagcgtga cgagcgcggt ccggtcgtca atcgcttcgg ccagcgcttc attcgagacg
     6301 ctgatgccgt cgccggagcg gacgatctcc agccggtgcc ccggcccggc caggttcagc
     6361 gcgctctgga ggacgtacac gtccgacggg aaattgagat cgtccgtcac gatcttcacg
     6421 cgcccagggc gcgcgcgcag cgcagccatg accagcttga agaagttaac ggtcgtcgaa
     6481 tcgccgacga tcacctcgtc gggcctcgcg ccgacgagct gcgccacttt ggatccgatc
     6541 cgctgcggcg cggtgaacca tccctcgccc cagccccgaa tgaggcgccg cccccattca
     6601 cgctcgacga cgtcgtggat gcgcgcggcg ctgcgcctcg gcatgcgccc cagcgagttg
     6661 ccgtcgaggt agatcaggtc gggatcgccg atcacgaact cgtcgtggaa gcgagcgagt
     6721 tcgtcttgtc ggtcgagttc gtgggcgtag ggggcgtcgg cgcgaatagg catgagttca
     6781 tcgattttgg ctagggcata agccgcttgt ccgcctatgc agtgcaccgg atcatgcatt
     6841 gccttgagga gcggctcaac tgcgcgcgcc tttaatctgg cgaagggctt tagcgactgt
     6901 ccagcgcacc caattatctt catcggccag cgcctgcgcg agcggctcga ccgcccgctc
     6961 gtccttaatc tcgcccaagg ccagggccgc gatcatgcgc acgatgctgc tctgatcact
     7021 tagcgccgcg atgagcggct ccactgcgcg cgcattctta atccgcccca aggccatcgc
     7081 cgccgaccaa cgtatcgtcc aggcctcctc gcgcaaaacc cggatcaacg gctcgaccgc
     7141 tcgcgtatcg ccaagcatcc ccagggctgt cacgatggcc aggcgtatct cgccgtgttc
     7201 gtcctccaac atctcgatga atggttcggc tgcccgctta tcgccgattt cgccgaggag
     7261 tctgaccgct gacaggcgca cctcaggatc cacatcctgc aacgcggcga tgcgttcctg
     7321 caccgatttc ctcctcttct ggaagggcag aaagagggac aaatccaagt gcaatgcggc
     7381 aatgcgttcc cgaaccgatt tcctcggcag gctcttccgg tgagcgtact cgacttctga
     7441 cggcagaggc ttgccgcgtt tcgtgagcgc ttctcgaatg gccgccagta gctcctgtgg
     7501 cccgaatggc ttggcgatgt agccgtctac tttgaaagcc tgcaagccca ggtctttatc
     7561 gacgctctgc gctttcgcgg tgaggataat cacggggatg tcgcgcagag cttcatcaga
     7621 cttcatctgt tcgtaaaatt tccagccgtc catgtctggc cgcatgaggt cctgagtgaa
     7681 gaggtcgatc gggtgcgtgt gcagcattgc ccacgcttcg tggctgtctc tggtgcccag
     7741 atgctcgtat cctgcccgtt tcaggatcag cccgatcaga gacaccacgt ccgggtcgtc
     7801 gtcaaggctc aaaatccgtg tcatcgggat tgtccctgtc agttatacct gcgtcttcgg
     7861 atccgagagg atgggagtga gcgatcaact accatacccc ggcactccca tactcaataa
     7921 gtccccagtc tcgtcctcac cgcccacagt tcctcaaaga accttggccg cacctgccgt
     7981 tcctcgaaga tacgattctt ttcctgtatt cggatgccgg ccgtttccgc taggtactcc
     8041 gggccgagga agccggtgcc cgaccgcgcg ccgatcatct tctcgaccat tctgactata
     8101 acacatacgt aacgaccaag gcaaaccacg gcaacgaatc gacatcggat aaacgggtgc
     8161 gatccgtacc gatgggccat ccgttaatcc gttgtgtatc tgctgctttg acccttcccg
     8221 cccgacgcgc ttgccctgat ggtgtaccca cgccccttga caactgtccg tcaccgtcca
     8281 tatactttct tcttgatacc ggggagattt gtgcatggcg gctcctcctc aacctgcgga
     8341 ccttgaggcg ctttcccgcc gcttccatcg gtttgcgacg ctggagaccg gcgggtcgtc
     8401 gccgctgtac gagcggctgt gcctgggtat ctcccaggat gccgatgtgt tggcgctggc
     8461 ggcgcaggcc cgcagtcacc ctgtgcccaa tatcctgctg gctgctgtcc actacctgct
     8521 cctcaaaggc gttcagcatc cgcttgccgc gttttacccg agcgtctcga acgagacgca
     8581 tccgggtggc gacccgtatc ctcacttccg cgcattctgc attgagcatc gtgatgagat
     8641 actgaacctc atcaggacac gattggttca gacgaacgag gtgcaccggt gcgcgtatct
     8701 cgcgcttgca ttcggcatcg tcgcagaacg ggcggggcat cgcccgctgg ccctgatcga
     8761 cgtcgggacg agcgccgggc tgaacttgct gtgggaccgc tttggatatg actacggcag
     8821 gcggaggctc ggcgatccag gcgcgcctgt ccagttgacg tgcgagcttc gcggcgtccg
     8881 gcgcccgccg atcccagaga ggctgcccgc cgtcgcatcg cgcgccggga tcgacctcag
     8941 cccggtcgac gtgcgtgacc cggatgcggt gctgtggttc cgcgcgctga tctggccgga
     9001 gcatcgcgag cgcgtcgagc tgctgatgcg cgcgattcag ctcgcccggc aagacccgcc
     9061 ggagttgatc gccggcgacg cgctcgagct gctgccacag gtgatcgctg ccgtcccacg
     9121 cggcacgcct gtctgcgtct ttcacacgca cactgccaac cagttcccgc gcgaggcgcg
     9181 cgaacagttg atcgcgctca tcgaagagca cggcagacgg cgcgacgtgt atcgcgtctc
     9241 agccgagtgg atcggcacgc cacaaacgca gttggagctg gtcgcgtacg agcagggagt
     9301 gaaaacggag aagctgctgg cctacgtcga cggccatgcg aggtggatcg agtggttgga
     9361 taagaggctg ccaacctctg gtacttgact cggccttcgc ttgtcttttt ccccaacttt
     9421 tctggccccc aaatgatagt attgtgtaga gggctatcaa cgttgatttg ccaaagggtt
     9481 actcatggag gaaagaccgg ccatagcccg cctcaagcag ggtgatatag gcggtctgga
     9541 aacgctcgtg cggatatacc aagtacgcgc tgttcgggca gcgtatctgg ttgtgcgaga
     9601 tcgcgcctta gccgaagaca tcgtacaggc cgcatttgtg cgcgcctacg aacgtatcgg
     9661 ccagtttgac gccaaccgcc ccttcgggcc ttggtttctg aaaagcgtaa tcaacgactc
     9721 gctcaaggct atcacacaac aacagcgcaa cgtatcactc gattgggaag cagagcgcga
     9781 agatacaaca tgggctgacc gcttgattga ccccgctcct ggccctctcg aaatggctga
     9841 aagtgccgat atgcagcgca tggtgcaaga ggcgctaggc caacttaccc ctgcacagcg
     9901 caccgctgtt gtcttacgct acttccttga cttcagcgaa gccgagatga cgacgaaact
     9961 gaatcgtcca cctggaacgg ttagatggtt attatacgcg gcacgggaac gcttacgtga
    10021 cattctcagc tcactattac ccgactctgg ctcccatggc tcgcaggtat caaagaacga
    10081 ctgatgcgag aggtgctcca tgcaacatga actcatccgg caaaatctgc gccgaatagc
    10141 cgaacaggcc gtacccgatg ataccgactt gtggccggct attcacgctc gacttatcgc
    10201 acctcgcagg agggggcact ccacggtcgg caatactttt aggcgagtgt tcgccttcag
    10261 cgcgatcaca ctagtcgtgt tcgcgacgat cttactaagt gtcccgccag cgcgggcagc
    10321 ggtattggaa ttccttcgcg agttgggcgg ttggattttc acagatgaac agacttcggc
    10381 tcaacaggta gtcgccgatc catctctcat cccaacggtc ctcccgaatg atggcgagga
    10441 agtcgaacgg ctcgatgtca ccgaaagtga agaacgtctc ggtgaatcga tatatgtgcc
    10501 aagttacatt ccgacaggct ataaacttat agcccgcact ggacccggac agaacgcctc
    10561 cgatgtggct tacatgatca catcgtatcg caatagcgca gaagcttctg atttcttcat
    10621 cgtccaaggt aaggtcaaag tgccacagca agcagacatg ggtgtggaac ccacagaagt
    10681 cttcgtgcgc ggccaaaccg gcttgtggtt tgagcgagcg ccgattagct atagcgcccg
    10741 atacgaagag ggcggcgatg taataatgga attagggata aggaatgtgc tggtgtggga
    10801 ggagggcgac ggttatctat cgatcagtag cagcaacttg cccctcgagg aaactctgaa
    10861 agttgccgag tccttgacga agtagtgtcc aagaggtgca ccccatcttt gacagccgca
    10921 ctagtagaac gcccggcttt ctatcgacgg aagccgagct ttgatgtttg cctaacagtc
    10981 aggagatgca tgctgtcggc ggcggcaatt atcagactct gatcaagcag gcgcgctgcg
    11041 ccgcaaaagg atggctgagg catggtcggc aaggcggttt tccaactgtt gtaaaatacc
    11101 cctgtgccat tcacgaccct cgtctccacg gcggaactct cacagcacct g
//
LOCUS       DBNHXB010000371         2390 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3459861, whole genome shotgun sequence.
ACCESSION   DBNHXB010000371 DBNHXB010000000
VERSION     DBNHXB010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3459861"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="AC162G_15120"
     CDS             complement(<1..790)
                     /locus_tag="AC162G_15120"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5077184.1"
                     /translation="MADLSGTTLGKYQLIERLGRGGMADVYKGYQPGLDRYVAVKVLH
                     PHLSEDPDFITRFRREAKSVADLRHPHIVQVFDFDIQGENYYMVMEYVEGGKTLKQLL
                     QEMATKGERLPLDRTLDIVARLADALAYAHGLGMVHRDIKPANVLLPSLERPVLSDFG
                     IARLLGETGLTGSGVMIGTPAYMSPEQGRGERGDARSDIYALGIVAFEMFTGKLPFQG
                     RNAQEMMIARLRGTPTPIRQIRGDVPAHVDRALMKSMATDPDGRF"
     gene            1226..>2390
                     /locus_tag="AC162G_15125"
     CDS             1226..>2390
                     /locus_tag="AC162G_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077185.1"
                     /translation="MGNEPREAERTQSANGLQQALWLFIAATWLLAACAPPTPEPTST
                     PVPSPTPTSTPTITPTPTRVPREACPPPDPNAAWIAPQDFSGYPRAIGGFLNAGASAD
                     ALRAILTNASSIGAQFGGVWPFDLTGDGAPETVVSIYDPLGEVYGPVPSGLLLVYGCV
                     DRAAPLLHEDTGPPMPQVARIADLIGAGRGGEIATIHSMCGAHTCFDTLDVIGWDGAG
                     FVSLMGDRLQMPYPTYTFVDLDGDPALEVQAVSGQIASAGAGPQRTATETWDWNGSQY
                     VKVAGSISSPEYRIHVVHDADDLLLSGDVAGAISLYSRVITDDTLRDWLLEIGVEKPD
                     DHANLTAYAWYRIMLAHTWSGDAASAQEAFDRLSADFPAGAPGHEYQELAQVFW"
BASE COUNT          419 a          763 c          767 g          441 t
ORIGIN      
        1 cgaagcgacc atcggggtcg gtggccatcg acttcatgag ggcccgatcc acgtgcgcgg
       61 gcacgtcgcc acggatctgg cggatcggcg tcggcgtgcc gcgcaggcgc gcgatcatca
      121 tctcctgcgc gttgcggccc tggaacggca gcttgccggt gaacatctcg aacgccacga
      181 tgcccagcgc gtagatgtcg ctgcgcgcgt cgccgcgctc gccgcgtccc tgctccggcg
      241 acatgtaggc cggtgtgccg atcatcacgc cgctgccggt caggcccgtc tcgccgagca
      301 ggcgggcgat accgaagtcg ctcagcactg gccgttcgag gctgggaagc aagacgttcg
      361 ccggcttgat gtctcggtga accatgccca ggccgtgcgc gtaggcgagc gcgtcggcca
      421 gtcgggcgac gatgtcgagc gtcctgtcga gcggcaagcg ctcgcccttc gtcgccatct
      481 cctgcagcag ttgcttgagc gtcttgccgc cttcgacgta ctccatcacc atgtagtagt
      541 tctcgccctg gatgtcgaag tcgaagacct ggacgatgtg gggatggcgc aggtcggcga
      601 cgctcttcgc ctcgcgccgg aagcgggtga tgaagtcagg gtcctcggac aggtgcggat
      661 gcagcacttt gacggcgacg tagcggtcca ggccgggctg gtagcccttg tagacgtcgg
      721 ccatgccgcc gcgcccgagg cgctcgatca actggtattt acctaaggtc gtgccggaga
      781 ggtcggccaa cgttgatcct ccgtagcgag aacattgtac tccgcttgta cccactgatg
      841 caagtgcttc accacctgcc acacgccatg atacaatcgt ctacgctggt caggcgtcat
      901 cgcgagtcct ccatggcaaa gaacgtcatc cgatatggca cacataggcg tgctacacaa
      961 tccgcgccag acgtgtcatg ccgaggagga acgacgaggc atctctatcc cggcgcgccg
     1021 cgctcgctga tcagagtaga gattcctcac tgcgttcgga atgacactgt cagaggtgtc
     1081 atgccgagga ggaacgacga ggcatctctg ccccggcgcg ccgcattcgc cggtcagacg
     1141 agagattcct cactgcgttc ggaatgacac gccacaagtg gccgaacagt taccgtctac
     1201 gtaagtgttc agaaggtgtc aacgaatggg caacgaaccc cgcgaagcgg agcgcacgca
     1261 gtcagcgaat ggcttacaac aggcactctg gcttttcatt gctgcgacct ggctgctggc
     1321 tgcctgcgcg ccgcccacgc cggaaccgac ttcgacgccc gtgcccagcc cgacgccgac
     1381 ttccacgcct acgataaccc cgacacccac gcgtgtccct cgcgaagcgt gcccaccgcc
     1441 cgatccgaac gcggcctgga tcgcgccgca ggatttcagc ggctatccca gggcgatcgg
     1501 cggtttcctc aatgcaggtg cctcggcgga cgcgctgaga gcgattctga caaacgccag
     1561 ctcgatcggc gcacagttcg gcggcgtgtg gccgttcgac ctgaccggcg acggcgctcc
     1621 ggaaacggtc gtctcgatct acgatccact gggcgaggta tatgggccgg ttccgagcgg
     1681 cctgctgctg gtttacggct gcgtcgatcg cgccgcgcct ctcttgcacg aggataccgg
     1741 cccgccgatg ccgcaagtgg cgcgcattgc cgaccttatc ggcgcagggc ggggcggcga
     1801 aatcgcgacg attcactcga tgtgcggcgc gcatacgtgc ttcgatacgc tcgacgtgat
     1861 cggctgggac ggcgcgggct tcgtcagtct gatgggcgat cgcttgcaga tgccgtaccc
     1921 cacttacacc ttcgtcgacc tggacggcga tccggcgctc gaggttcagg cggtgagcgg
     1981 tcaaatcgcg tcggccggcg caggaccgca gcgcacggcc acagagacct gggattggaa
     2041 cggctcgcag tacgtcaaag tcgccggatc catctcgtcg ccggagtatc gcattcacgt
     2101 cgtccacgac gccgacgatt tgctgctgag cggtgacgtc gccggcgcga ttagtttgta
     2161 cagtcgcgtg atcaccgatg atacactcag ggattggtta ttggagattg gggtcgaaaa
     2221 gccggatgat cacgccaatc tcacggcgta tgcctggtat cgtatcatgc tagcccatac
     2281 gtggtcaggc gatgcggctt ctgcgcagga ggcgttcgat cgcctgagcg cggatttccc
     2341 ggccggcgcg cccggtcacg aatatcaaga attggcgcag gtcttctggt
//
LOCUS       DBNHXB010000372         6160 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3795519, whole genome shotgun sequence.
ACCESSION   DBNHXB010000372 DBNHXB010000000
VERSION     DBNHXB010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6160)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3795519"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="AC162G_15130"
     CDS             complement(<1..537)
                     /locus_tag="AC162G_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR5077186.1"
                     /translation="MKKRRMLILGSVGVLIAAGIVIVTNNQSSTAQADAELGQVTRAT
                     LSSVVESSGSVIPESDVTLSFDTAGTVAAVNVQPGDRVKQGDVLAELDTRSLELQVAK
                     REQAYLIQQASYSLTVQPDPAEVTAAQAALNNAWEAYALAQQKVAVNRTDQVMLSCLN
                     VENARRTYDDAVTAYNTYL"
     gene            829..1176
                     /locus_tag="AC162G_15135"
     CDS             829..1176
                     /locus_tag="AC162G_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077187.1"
                     /translation="MDDAKDFASAILDKMVSFGKAQFGDAVKAYWIYNGDVCPCCLKR
                     PIDVMKYKGKEALSVNGFMYRERGVLIGYLLCGLCAQDIFKTSKKRQTSMHENIERNL
                     ISAYQKHLASLDA"
     gene            complement(1501..2670)
                     /locus_tag="AC162G_15140"
     CDS             complement(1501..2670)
                     /locus_tag="AC162G_15140"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR5077188.1"
                     /translation="MRAIAKHQLIILSIIAIFAVTACSPAVTSNPVPVAATAGAGAVS
                     PTLPPEAESQTSQPGATIPINAPEAVAADDLQAAIIGVYQQANLAVVYIIVPSVGSGS
                     GFVYSQDGYIVTNRHVVAGGRNFEVVFANGERQRAELVGSDTDSDLAVLKVDRLPDGV
                     GPLALGDSNSIQVGQFVVAIGNPFGEQGSISFGIVSGVGRSLPSQRETTSGSTYALPE
                     VIQTDAPINPGNSGGPLLNLAGEVLGVNAAIATTTGTNSGVGFSIPVSAIKLIVPSLI
                     GDGEYVYPYMGAGFDGDITLDDQSDYGLSQTQGAYVINVTSGSPADNAGLIPANGNTG
                     RGGDLVVAIDGRSIVDFADLNSYLVFQTAVGQTIEITVLRDGERVVLPLTLGARP"
     gene            complement(2773..3336)
                     /locus_tag="AC162G_15145"
     CDS             complement(2773..3336)
                     /locus_tag="AC162G_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077189.1"
                     /translation="MSKFLKFAGMVTLVAAMVIALGAIWPASAQTAMLAAPAARLAHG
                     GGFSRGMCGEAGLEAAAEALGMTTDELSTQLWGGKTLADLADEAGVELQAVRDAVEAA
                     CEQAIRDAIEQAVTDGQLTREHADWLLEGLDNGYWGSGSGYGFGGHGFGPGGFRGGGF
                     HGFRGGMGFHGFGFPNSNSVTPDSSGL"
     gene            complement(3539..4075)
                     /locus_tag="AC162G_15150"
     CDS             complement(3539..4075)
                     /locus_tag="AC162G_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077190.1"
                     /translation="MSKFLKFAGMATLIVAVVVAMGAISPAFAQAATTSAPAARLAHG
                     GGFGFGPYGQEALEAAAETLGMTADELSTQLWGGKTLAELAEEAGVDLQDVRDAVEAA
                     QLEAVRAAIAQAVEDGSITQAQADWLLEGLDNGYWGGGHGMGPGFGMGRGGFFGPRGF
                     GGFGAPSSNGTTTGTSGA"
     gene            complement(4179..5570)
                     /locus_tag="AC162G_15155"
     CDS             complement(4179..5570)
                     /locus_tag="AC162G_15155"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5077191.1"
                     /translation="MNRLWVRLTLAFALAVLIGIGTVAVLADIAIGREFRSFIARNEA
                     DLQSSALATELVSHFEAHPNWEGVDQVISQFTPRRGHDIGSPPPDSAPGGGRAGPQLV
                     VADAAGRIVYDSYDRRLNEFLSSMERELAAPLQADENTIGYLAILPGPIDSLRPVELN
                     FIAGSRQNLFIAALLAGGVAIVLGVAFSRSLAQPLNRLAAAARAIAAHDLTQRVQPGG
                     TAEVVEVGHAFNEMAASLEKAEELRRNLVADVAHELRTPLSVLQGNLTGLLDGVFPLE
                     MTEVARLYDETRLLSRLVDDLRELAQAEAGQLHLDLRSMNVADVVRSTVEAFSAVAAE
                     HQVILTGDAPSDLPPVRADPERTAQVLRNLLANALRHTSPGGAIRVTTSSRPGWVEVS
                     VSDTGEGIAVEDLPHVFDRFWRGDKSRARETGGSGLGLAIARRLIEAQGGQIGAESEL
                     GKGSRFWLRLPLV"
     gene            complement(5580..>6160)
                     /locus_tag="AC162G_15160"
     CDS             complement(5580..>6160)
                     /locus_tag="AC162G_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5077192.1"
                     /translation="HIIRRDRPDLIVLDLMLPDRDGWSITRIVRGDESLARTPIIMLT
                     ARVEDGDKIAGLESGADDYIPKPFNPNELVARVRAVLRRAGNHFGQSHVIQFGSILLD
                     VDRHEVQIGGRPVELTVTEFNILRALMENPGHAFTRLELIEKGLGYTYEGMERTLDSH
                     IKNLRKKIEPDPSSPTYIHTVFGVGYRMSLRE"
BASE COUNT         1184 a         1919 c         1871 g         1186 t
ORIGIN      
        1 caggtaggtg ttgtacgccg tcaccgcgtc gtcgtaagtc cgcctggcgt tctcgacatt
       61 gagacaactg agcatcacct gatcggtgcg gttgacggca accttctgct gcgccagcgc
      121 gtaagcctcc caggcgttgt tcagcgcggc ctgcgccgct gtcacttcgg ccgggtcggg
      181 ctggacggtc aggctgtaac tggcctgctg gatcaagtaa gcttgttccc gcttcgccac
      241 ttgcagctcg aggctacgcg tatccagttc cgccaggacg tcgccttgct tcacgcgatc
      301 gccgggctgc acgttcacgg cggcgacggt accggcggta tcgaacgaca aggtgacgtc
      361 ggattcgggg atgacgctgc ccgaagactc gacgaccgac gacagcgtgg cgcgtgtcac
      421 ctggccaagc tcggcatccg cctgcgccgt gctgctctgg ttgttggtga cgatcacgat
      481 ccccgccgca atcagcacac ccacgcttcc aaggatgagc attcttcgct tcttcattga
      541 tgcatactcc tacgcggcat aggttaaacc gcgggccatt tcagcacctg aatccggcat
      601 catatggttg tgtggagcca gtatggagac gatatgaggg tcaggggtgc agataagaat
      661 tgagcgaacc atgtcgatta tctccccagg gtaagtttcg ggcaaccaga ggatgggtta
      721 agttggcaag agtctgttgc tcgtgtaaga ttaaggttgg tgtttgcgac cattggcgcg
      781 tgcgctggcg tgcactgcgt gatttcgccc ccttggaggt atgacctgat ggacgacgct
      841 aaagatttcg cctcggcaat tctggataag atggtttcct ttggtaaagc ccaatttgga
      901 gacgccgtta aggcttattg gatttacaat ggagatgtgt gtccttgctg cctgaaacgt
      961 ccgatcgatg tgatgaaata caagggcaag gaagcgctgt ccgtcaacgg cttcatgtac
     1021 cgcgagcgcg gcgtgctgat cggctatctg ttgtgcggcc tgtgcgcaca ggacatcttc
     1081 aagacttcca agaagcggca gacgtcgatg cacgagaaca tcgagaggaa tctgatcagc
     1141 gcctatcaga agcatctggc gtcgttggat gcttgagagg cacgcccgtt cacaggttgt
     1201 tcggagcagc ataggctaaa acctcgttca gcggcctaac gccccaaagc tcaagcgcgt
     1261 acaaacagac tgtgccagat gtgtcatgcc gaggaacgac gaggcatctc tgccccggcg
     1321 cgccgcgttc gccggccgga ggagagattc ctcactgcgt tcggaatgac actacccgac
     1381 acctgacacc tgacacctga cacccgatac ctgaaacccg acggagtaga gattcctcac
     1441 tgcgttcgga atgacacgcc acaagtggct gggcagttac cgaaaagtaa gccatcacgg
     1501 tcacggacgg gctcccaacg taagaggaag cacgaccctt tctccgtcac gcaagacggt
     1561 gatctcgatg gtctgtccaa ccgctgtctg aaacacgagg tagctgttca ggtctgcgaa
     1621 atcgacgatg ctccggccgt caatcgcgac gaccagatcg ccgccccggc ccgtgttgcc
     1681 attagccggt atgagacccg cattgtctgc tggacttccg gatgttacat tgatcacgta
     1741 agcgccttgt gtctgtgaca gaccataatc agattggtcg tcgagtgtga tgtcaccatc
     1801 aaaacccgct cccatgtagg ggtagacata ctctccgtct ccaatcaggc tgggcacgat
     1861 cagcttgatg gcgctgaccg gaatcgaaaa gccgacgccg gagttcgtgc ctgtcgtcgt
     1921 ggcgatggcg gcgttgacgc ccagcacttc gcccgccagg ttcagcagtg ggccgcccga
     1981 gttgcccgga ttgatgggcg catcggtctg gatgacttcc gggagcgcgt acgtgctgcc
     2041 ggacgtcgtt tcgcgctgag agggcaggct gcgcccgaca ccgctgacga tcccaaagga
     2101 gatggagcct tgttcgccga acgggttgcc aatcgcgaca acaaactgac cgacttggat
     2161 gctattcgag tcgcccagcg cgagtggtcc aacaccgtcg ggtaagcgat cgactttgag
     2221 gactgccaga tcgctgtccg tatcgctgcc gacgagctcg gcgcgttgcc gctcgccatt
     2281 cgcgaacacc acttcgaaat tgcgcccgcc cgcgacgaca tgccggttcg tgacaatata
     2341 gccgtcctgg ctatacacga atccactgcc actcccgacg gacggaacga tgatgtaaac
     2401 gacagccagg ttggcttgct ggtagacgcc gatgatcgcc gcctgcaagt catcagcagc
     2461 aacagcttcc ggggcattga tgggaatggt tgcaccgggt tgacttgttt gtgattcagc
     2521 ctcgggcggc agcgtgggcg agacggcgcc cgcgcccgcc gtggccgcaa ccgggaccgg
     2581 attggacgtt actgccgggc tacaggccgt gacggcgaag atggcgatga tgctcaagat
     2641 gatgagttga tgcttcgcta tggctctcat ggtactcatc gtttcgttac tccgaaggaa
     2701 ttggctcgtt tcagcacatg agcgcagcca tcgctacgct catgtgcctt acacaatgct
     2761 tcttggaaac gcctacaagc cgcttgaatc gggggtcacg ctgttgctgt tcgggaagcc
     2821 gaagccgtga aagcccatgc cgcctcggaa gccatggaat ccgccgccac ggaaaccgcc
     2881 cgggccaaag ccgtgcccgc caaagccata gccggacccg ctgccccagt agccgttatc
     2941 gagaccctcc agcagccaat ccgcatgttc gcgggtcaac tgtccgtccg tgacagcctg
     3001 ctcgatggca tcccggatgg cctgctcaca cgcggcctcc acggcgtctc ggacggcctg
     3061 cagttccaca ccggcctcgt ccgccagatc ggccagtgtc ttgccgcccc acaactgcgt
     3121 ggacagctcg tcggtggtca tgccgagcgc ctcggccgcg gcctccagcc ctgcctctcc
     3181 gcacatccct cgactgaagc cgcctccatg cgccagcctg gctgcggggg cggcgagcat
     3241 tgctgtctgc gcagaggccg gccaaatggc tcctagcgca atgaccatcg cggcgaccag
     3301 cgtcaccatg cctgcaaact tcagaaactt tgacaatctg cacctcctcg ctggaaatca
     3361 ggtttacctt gtacagcttt ccgcctgtat tatagccgct gaagatggag attttatgga
     3421 gaaaagctaa aaggatgatt aaacgagggt ggagcagatg agcgtagcga cacgctacgc
     3481 tcatccattg cttctattcg attttcatct gttgtcgcac tttgttatcg cacctcgatc
     3541 aggcgccgct ggtcccggtg gtcgtgccgt tgctgctcgg cgcaccgaag ccgccgaacc
     3601 cacgcgggcc gaagaatccg ccccggccca tgccgaagcc tggccccatg ccgtggccgc
     3661 caccccagta gccgttatcc aggccctcca gcagccagtc cgcctgcgcc tgcgtgatgc
     3721 tgccatcctc gacggcctgc gcgatggcgg ctcggacggc ttccaactgc gccgcctcga
     3781 cggcatcccg cacatcctgc agatcgacac cggcctcttc cgccagctcg gccagcgtct
     3841 tgccgcccca cagttgggtg gacaactcgt cggcggtcat gccgagcgtc tcggcggcgg
     3901 cttccagcgc ctcctggccg tatgggccga acccgaagcc gcccccatgc gccagcctgg
     3961 ccgcaggggc ggaggtcgtc gctgcctgcg cgaaggccgg cgaaatggcg cccatcgcaa
     4021 cgacgacggc gacgatgaga gtcgccatcc ctgcaaattt cagaaacttg gacatcctgc
     4081 acctccattc tcacagttag agtctagcct gttcagctat ctgcctacag aatagctgct
     4141 ggaggtggag attttgcgga gcggagttaa ggaaatgatt aaacgagggg gagcctgagc
     4201 cagaaccggc tgcccttgcc gagttcgctc tcggcgccaa tctgaccgcc ctgcgcctct
     4261 atcaggcggc gggcaatcgc caggcccaaa ccggagccgc cggtttcgcg cgcgcgcgat
     4321 ttgtcgccgc gccagaagcg atcgaagacg tgtggcagat cctccacggc gatgccttcg
     4381 ccggtgtccg acacgctcac ctcgacccaa ccgggcctac tcgaagtggt gacacggatc
     4441 gcaccacctg gagacgtgtg gcgcaacgcg ttggccaaca ggttgcgcaa tacctgggcc
     4501 gttcgttctg gatcggcgcg aacgggcggc agatccgaag gagcgtcgcc ggtcagtatg
     4561 acctggtgct cggcggcgac cgccgagaaa gcctcgacgg tggatcggac tacgtcagcg
     4621 acgttcatcg agcggagatc aaggtgcagt tgcccggcct cggcctgcgc cagttcgcgc
     4681 agatcgtcga ccaggcggct gagcaggcgt gtttcgtcat acagccgcgc gacctcagtc
     4741 atctcgagcg gaaatacacc gtcgagcaac ccagtcagat tgccctggag aacgctcaac
     4801 ggcgtgcgca gctcgtgggc cacgtcggcc accaggttgc gccggagttc ttcggccttt
     4861 tccagcgagg ccgccatctc gttgaaggca tggccgacct cgacgacctc ggcggtgccg
     4921 cccggttgca cccgctgcgt caggtcgtgc gccgcgatgg cgcgcgcggc cgccgcgagt
     4981 cgattcaacg gctgcgccag gctgcggctg aaggccacgc ccagcacgat cgcgacgccg
     5041 cctgcgagca gcgccgcgat gaagagattt tgccgcgagc ctgcgatgaa gtttagctcc
     5101 accggtcgca acgagtcgat cgggccgggc agaatcgcca ggtagccgat cgtgttttcg
     5161 tccgcctgca gcggcgcggc gagttcgcgc tccatagatg agagaaattc attcaggcgc
     5221 cggtcgtagc tgtcgtacac aatgcgaccg gcagcatcgg ccaccacgag ctgcggtccc
     5281 gcgcgacctc cgcctggcgc gctgtcaggg ggaggggaac caatgtcgtg accccggcgc
     5341 ggcgtgaact gggatatcac ctgatcgacg ccctcccaat ttgggtgtgc ctcgaagtgc
     5401 gagacgagtt cggttgccag cgcgctgctt tgaagatcgg cctcgttgcg cgcgatgaaa
     5461 gagcggaact cgcgtccaat ggcgatatcc gccagaaccg ccaccgtgcc gataccgatc
     5521 agcacggcga gcgcaaaagc gagtgtcaga cgtacccaca gtcgattcat tccattgccc
     5581 tactcacgca ggctcatccg gtagccgacg ccgaagaccg tgtgaatgta agtcgggctg
     5641 gacggatcag gctcaatttt cttgcgcagg ttcttgatat gactgtcgag tgtgcgctcc
     5701 atgccttcat aggtgtagcc caggcctttc tcgatcagct ccaggcgcgt aaaggcgtgg
     5761 ccgggattct ccatcagcgc ccgaaggatg ttgaactcag tcaccgtcaa ttcgaccggc
     5821 cggccgccga tctggacttc gtgccggtcg acatcgagca gaatcgagcc aaactgaatg
     5881 acgtgggact ggccaaagtg gttgcctgcc cgccgcagaa cggcgcgcac acgcgcgacc
     5941 agctcattcg gattaaaggg tttggggatg tagtcgtcgg cgccggattc caggccggcg
     6001 atcttgtcgc cgtcttcaac acgggcggtg agcataatga tcggtgtgcg agccagcgac
     6061 tcgtccccac gcacgatgcg cgtgatgctc cagccatcgc ggtcgggcag catcaggtcg
     6121 agcacgatca ggtcaggccg gtcgcggcga atgatgtgca
//
LOCUS       DBNHXB010000373         2354 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3963973, whole genome shotgun sequence.
ACCESSION   DBNHXB010000373 DBNHXB010000000
VERSION     DBNHXB010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3963973"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="AC162G_15165"
     CDS             complement(<1..320)
                     /locus_tag="AC162G_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006554858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR5077193.1"
                     /translation="MSTRKVRVAVNGYGVIGKRIADAVAAQDDMELVGVADVVSDWRI
                     KVAVEKGYPVYAATPQATEQMRAAGIPLVGNLDELLGWVDVVADATPKKVAAANLEKY
                     RAAGV"
     gene            complement(379..714)
                     /locus_tag="AC162G_15170"
     CDS             complement(379..714)
                     /locus_tag="AC162G_15170"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR5077194.1"
                     /translation="MSAVRPVDSPAKMPSADERRCVRATRLVVWGMVCPNCATRIHGC
                     LAALAGVVEVQVDHNVGMAYVTYDVTLITVPALIDAVQRAGSDRWHTYGATESDVPYS
                     GLRRHTQRR"
     gene            complement(751..1812)
                     /locus_tag="AC162G_15175"
     CDS             complement(751..1812)
                     /locus_tag="AC162G_15175"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077195.1"
                     /translation="MSRPRGTIGLILGVGVVLLVFFAIEGLWAGVGRTLSVQDTEALV
                     VLPKDSLGLSGSLMPIGLKTTLRGMGAEVVAPQLFTTRQIRPGEPVLLRGIALDNGST
                     GLARIVAFEMTAGAPLERGDRDKVMVGAEVARSKGLAPGDRFRVLGKTFIVKGVFATG
                     SISDSEVWLDLGEAQRLVRSRGFASMFSVIGPPGLAERIAQRLDVDVVKEKEVWESLT
                     GAIASLDGVMKVASVIVAAAAVLGVMNTIFTVVQQRRREIAILRSMGFGRSAVLAYVL
                     SQSLLIALGGFVIALAAAALLLRLMRMEAVGMTITPVLTTAGILRGLGLTLVIGVLAG
                     LYPALRAARLNIAQTLRGE"
     gene            complement(1903..>2354)
                     /locus_tag="AC162G_15180"
     CDS             complement(1903..>2354)
                     /locus_tag="AC162G_15180"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077196.1"
                     /translation="TEPEVYETMNRSFGQVTRVMNAISALALIAIAFGVYNVVGMTVA
                     EKAHEIAVLKSIGVSARQVIGIFVQEGLIQAGLGFAIGLAAGGAALAYLNATSAVSFL
                     SVPLTLSLAPQTVLVGGALTAALTLASAWLAARRVAAAPVAEVLRSI"
BASE COUNT          451 a          827 c          667 g          409 t
ORIGIN      
        1 acgcctgccg cgcgatattt ctccaggttg gccgcagcga ccttcttagg tgtggcatcg
       61 gcgacgacat cgacccagcc gagcagctcg tccagatttc cgacgagggg aataccggct
      121 gcgcgcattt gctcggtggc ctgcggcgtt gccgcataca ccggatagcc tttctcgact
      181 gcgaccttga tgcgccaatc gctgaccaca tcggccacgc cgaccaattc catgtcatcc
      241 tgcgcggcaa ccgcgtcggc gatgcgcttg ccgatgacgc cgtagccgtt gacggccacg
      301 cgaacttttc gtgtagacaa gtatgcctcc tgtaattgtg tgtggaagat tataacggat
      361 catctcagca tagacgtctc accttctctg tgtgtgacga cgcagaccgg agtacggaac
      421 atcgctctca gttgccccat aggtgtgcca tctatcagac ccggcccgct gcacggcatc
      481 gatcagcgcc ggaacagtaa tcagggtcac atcgtacgtg acgtacgcca tgcccacgtt
      541 gtgatcgacc tggacttcca cgacaccggc aagcgcggcc aggcatccgt gaatgcgagt
      601 ggcgcagttg ggacagacca tgccccacac cacgagccgc gttgccctga cgcaccgccg
      661 ttcgtcggca gatggcattt tcgcgggtga gtcgaccggc ctgaccgcgc tcatgagaat
      721 ctcaatttca acatgatttg gatagtcatc tcattcccct ctcagcgtct gcgcgatgtt
      781 gagccgcgcc gcacgcagcg ccggatacag cccggccagc acgccgatga cgagcgtcag
      841 ccccagcccg cgcaggatgc ctgcagtcgt aaggacgggc gtaatcgtca tgccgaccgc
      901 ctccatccgc atcagccgca gaagaagcgc cgccgcagcg agcgcgatca caaatccccc
      961 tagcgcgatc agcagcgact ggctcaacac gtacgccagc acggcgctgc gaccgaatcc
     1021 catgcttcgc aggatggcga tctcgcgccg ccgctgctgc acgacggtga agattgtgtt
     1081 catcacaccg agcacagccg ccgctgcgac gatgaccgat gctactttca tcacgccgtc
     1141 caggctggcg atcgcgccgg tgagcgactc ccatacttct ttctctttga ccacatctac
     1201 gtcgagacgc tgcgcgatgc gctcggctaa ccccggcggg ccgatcaccg aaaacatcga
     1261 ggcaaatccc cgcgagcgca cgagtcgctg tgcttcgcct aaatccagcc acacttcgct
     1321 atcggagatg ctgccggtgg cgaatactcc cttgacgatg aacgtcttgc ccagcaccct
     1381 gaaccggtcg cccggcgcaa gtcccttcga gcgcgccacc tctgcgccga ccatcacctt
     1441 gtcccgatcg ccgcgttcga gtggcgcacc tgcggtcatc tcgaacgcga cgatgcgtgc
     1501 cagccctgtc gatccattgt cgagcgcgat cccgcgcaac aggactggct cgccgggtct
     1561 aatctgccgg gtcgtgaaca gttgcggcgc gacgacctcg gcgcccatgc cgcgcagcgt
     1621 cgtcttcagg ccgatcggca tcaggctgcc ggagaggccg agcgaatctt tgggcagcac
     1681 caccagcgcc tcggtgtcct gcaccgacag tgtgcgtccg accccggccc acagcccctc
     1741 gatcgcgaaa aacaccaaca gcacgacgcc cacgccgagg atcagcccga tcgtcccgcg
     1801 ggggcgcgac accaactctc tccacgcgat cttgaccatc tcagcctccg agttccttca
     1861 gttccttaca gcaggcgcag gaactgaagg aactttggga actcatatgc tgcgaagcac
     1921 ttcggccacc ggcgcggcgg ctacgcgccg ggctgccagc catgccgagg cgagtgtcag
     1981 cgcggccgtc agcgcgcccc ccacgagcac cgtctgcggc gccaggctca gcgtcagcgg
     2041 cactgacaga aacgacacgg ccgaggtggc gttcaagtaa gccagggcag ctccacccgc
     2101 cgccagcccg atggcaaacc ccagcccggc ctggatcaac ccttcctgca cgaagatgcc
     2161 gatcacctga cgggccgata ccccgatcga cttgagcacg gcgatttcgt gcgccttctc
     2221 ggcgacggtc atccccacca cgttgtacac gccgaaggcg atggcgatca aggccagcgc
     2281 ggaaatcgcg ttcatcacgc gcgtcacctg cccgaacgag cgattcatgg tttcatagac
     2341 ttccggctcg gtgt
//
LOCUS       DBNHXB010000374         4231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2119356, whole genome shotgun sequence.
ACCESSION   DBNHXB010000374 DBNHXB010000000
VERSION     DBNHXB010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2119356"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..818
                     /locus_tag="AC162G_15185"
     CDS             315..818
                     /locus_tag="AC162G_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR5077197.1"
                     /translation="MKEPVLVLNRNFEPLNVCNTRRAVGLLLLGKAEIIENGRGEIRT
                     ASAAFPRPSVIRLSHFIHRPRPRVRLTKREVFRRDEYRCQYCGTANAHLTIDHVVPRH
                     RGGGHDWSNLVAACPPCNRRKGGRLLQDAHMRLLKPPGEPHPTADYLFGRYAHSFAEW
                     SKYLQGW"
     gene            1131..1898
                     /locus_tag="AC162G_15190"
     CDS             1131..1898
                     /locus_tag="AC162G_15190"
                     /inference="COORDINATES: protein motif:HMM:NF018290.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR5077198.1"
                     /translation="MALKIAISSQKGGVAKTTTTLSLGAALAECGRSVLLVDLDPQAN
                     LTQSLGVDPDQLRRMIGDVLIDQASMLSVSRESSVFNLDLAPANHGLLVLDKVLYGRE
                     GYEYRLKTSVQSINGGFYDVVIFDCPPAFGTLTINALTASDLLIIPIPCEYYSARSLR
                     SYLSLLHLVHRKTNPALRYRLLITMYDGRSRISRILLDQFRKTYGDTLFKTFISIDVR
                     LRESPIFGKPITQYAPNARGAQDYRALAAELMADATH"
     gene            1885..4005
                     /locus_tag="AC162G_15195"
     CDS             1885..4005
                     /locus_tag="AC162G_15195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family glycopolymer transferase"
                     /protein_id="HIR5077199.1"
                     /translation="MRPTELEQRLERLLSPPSGDGHSEMVPPLPVLRTAPVAGPVSAQ
                     ERAAHDGHPADVTTTAPVAGAAVGDLGRHKAILDQIPDPVCIKDRNHKWQVVNAAFCD
                     LLGRTEDELIGCAEHDFFPQAFADEAWALDDLVFETGQPVTVERPVESADGAHVWRIA
                     LRGQPLPEVEGSAQVLVVTIQDVTAQRQAEHALEHNQSALQAERKEWEERLRKLKRGM
                     LFQFSVFGILAGVSLGLVVAAGIPGRAAANALSTPTALVAVTATTPAPTEAAPTIAPE
                     VTDSVTPSPTISPTPTARSIAVTPAPTVATFTEDAPQPAPLREFGPQAINIVLMGSDR
                     RPGDGAWRTDVLILVSIDPGVPSVTMLSFPRDLWVYIPGWRWQRINLADGRGESAGFP
                     GGGPALVKQTIQYNFGIPVHYYARVDFAGYKRLIDSAGGIDVVADCPLYDIFPDVPDG
                     ANDIITGPELATVVTGTIDIPAAGVYHLDGKHALWYARSRKTTSDFDRSRRQQRVLRA
                     LWSEMREQGLLGQAPQLWDTLVQFVETDLTLNDVLYLADVAARLDTAHIRSRFIDGSM
                     LAWFATETGASVLRYDYSVLEPYLDQTFAPFPLNAASQKRAAVRVVNRAGHPDWEIVA
                     ADRLASAGFNVAEMSAENAVAPHTTIVDYTLTPKGSRLNDLIESLQVAPESAASRPAE
                     NSRVDYEVILGADFEPCQRPSRGW"
BASE COUNT          739 a         1458 c         1268 g          766 t
ORIGIN      
        1 tcgccgccag cgcgccggcg atgaccagtg cgccaacgcc gatcaggatg aatccgagct
       61 gccgcttcgt gatcacgccg gagattatag cacgctcgtg gatagatttc gggctgtaga
      121 cttcggaagt cttatggctt atgctcgctg gcgatgacgc ccgaaagact tccgaagtct
      181 gggctgtatt ttttgacgct cgccgggctt cgtgctatac tgcgcgccgt aacggcgggc
      241 gagtgcgacg cttgcggcgg tccctcgtcc gccttcgctt tgcccggacg taactgctct
      301 cgttatcaaa agagatgaaa gaacccgtac tcgtcctgaa tcgcaacttc gagccgctga
      361 acgtgtgcaa cacgcggcga gccgtcgggc tgctcctgct gggcaaggcc gagatcatcg
      421 agaacggccg cggcgagatc cgcaccgcgt cggcggcgtt cccgcgccct tcggtcatcc
      481 gcctgtcgca cttcattcac cgcccgcgcc cgcgcgtcag gctgacgaag cgcgaggtct
      541 ttcggcgcga cgagtatcgc tgccagtact gcggcacggc caacgcccac ctgactatcg
      601 atcacgtcgt tccacgccac cggggaggcg gccacgactg gagcaacctg gtggccgcct
      661 gccccccgtg taaccggcgc aagggcgggc gcctcctgca ggatgcgcac atgcgactgc
      721 tcaagccgcc gggcgagccg catcccacgg ccgattacct gtttggccgc tacgcgcaca
      781 gcttcgccga gtggtcgaaa tacctccagg gctggtagcc accacagtat atgggccttg
      841 tgtactatgc ggccggggag tctgctcctc ggccgcgttg cattaacact cccttcaccc
      901 atctgtaacc cccgacgccc gccgcttctc ctacaatcga atggaacggg taactgctca
      961 gcccgcgtca ttgcgagcgc tgttagcgaa gcaatctcct gcacggacag ccctgtgcgc
     1021 tcagggcggc tgagattgct tcgtcgcaca cggcgctcac ccgccccgcg cttctcagcg
     1081 gggtcgcaat gacggctaag tagttactgg aacggttgag gtgatatgcg atggccctga
     1141 aaatcgcgat cagcagtcaa aaaggcggcg tggccaaaac gacgacgact ctgtcactcg
     1201 gcgccgcgct ggccgagtgc gggcgcagcg tgctcctcgt cgatctcgac ccgcaggcga
     1261 atctcaccca gtccctcggc gtcgatcccg atcagctccg gcgcatgatc ggggacgtgc
     1321 tgatcgacca ggcgtcgatg ttatcggtca gccgtgaaag ctccgtcttc aatctcgacc
     1381 tggcgccggc caaccatggc ctgctcgtgc tcgacaaggt gttgtacggc cgcgagggct
     1441 acgagtaccg cctgaagacg tctgtgcagt cgatcaacgg cggcttctac gacgtcgtga
     1501 tcttcgactg cccgcccgcg tttggcacgc tcaccatcaa cgcgctgacg gcctccgatc
     1561 tgctgatcat accgatcccg tgcgagtatt actccgcccg ctcgctgcgc agttacctca
     1621 gcctgcttca cctcgtccac cggaaaacga atccggccct gcgctatcgc ctgctcatca
     1681 cgatgtacga tgggcgcagc cgcatcagcc gcattctact cgaccagttt cgcaagacgt
     1741 acggcgacac gctgttcaag acgttcatct cgatcgacgt gcgcttgcgg gaaagcccca
     1801 tattcggcaa gccgatcacg cagtatgccc ctaacgctcg cggcgcgcag gattatcggg
     1861 ccttagcggc ggagctgatg gccgatgcga cccactgagc tggagcagcg cctggagcgg
     1921 cttctctcgc ctccctccgg cgatggccac tccgagatgg tcccacctct gcctgtcctc
     1981 cgaacggcgc cggttgccgg gccggtctca gcccaggagc gcgcggcaca cgacggccac
     2041 ccggcggacg tgaccacgac ggctcccgtt gccggcgccg cagtgggcga tctggggcgt
     2101 cacaaggcca tcctcgacca aatcccggac cccgtatgca tcaaggaccg caaccacaag
     2161 tggcaggtgg tcaacgcggc tttttgcgac ctgctcggac ggaccgaaga cgagctgatc
     2221 ggctgcgccg agcacgactt cttcccacag gcgtttgcag acgaggcgtg ggcgctggac
     2281 gatctggtct tcgaaaccgg ccagcctgtc accgtcgagc gaccggtcga atctgctgac
     2341 ggtgctcacg tgtggcgcat cgcgcttcgc gggcagccgc tgccggaagt agagggatcg
     2401 gcgcaggttc tagtcgtgac gatccaggac gtcaccgcgc agcggcaggc ggagcacgca
     2461 ctcgagcaca accaatcggc tcttcaggct gagcgaaaag agtgggaaga aagactgcgc
     2521 aagctgaagc gcggcatgct gtttcagttc agcgtattcg gaatcctggc cggggtatcg
     2581 ctcggtttgg tcgtcgcggc gggcatcccg ggtcgcgcgg cggcgaatgc cttgagcaca
     2641 cccacagcgc tcgttgccgt caccgcgacg acgcctgcgc caactgaagc tgctcccacg
     2701 attgcgccgg aggtgacgga ttccgtcacg ccgtcgccga cgatttcccc cacgccgacg
     2761 gcgcgctcga tcgccgtcac acccgctccg accgtggcca ccttcaccga agatgcgccg
     2821 cagcctgcgc cgctccgcga attcggtcca caggcgatca acatcgttct gatgggcagc
     2881 gatcgacgcc cgggcgacgg cgcgtggcgc accgacgtcc tgatactcgt cagcatcgat
     2941 ccgggcgtgc cctctgtcac catgctgtcc ttcccgcgcg acctgtgggt gtacattccg
     3001 ggctggcgct ggcagcgcat caacctggcc gacgggcggg gcgagagcgc cggctttccg
     3061 ggcggcggcc cggcgctggt caagcagacc attcagtata acttcggcat cccggtgcat
     3121 tattacgccc gcgtcgattt cgccggctac aagcggttga tcgattcggc cggcggcatc
     3181 gacgtcgtcg ccgattgccc gctgtacgac attttcccgg acgtgcccga cggcgcaaac
     3241 gacattatca ccggaccgga gcttgccacg gtcgtgaccg gcaccatcga cattcccgcc
     3301 gccggcgtgt atcacctcga cggcaagcac gcgctgtggt acgcgcgctc gcgcaaaacg
     3361 accagcgact tcgaccgctc gcggcgccag cagcgcgtcc tgcgcgcgct gtggagcgag
     3421 atgcgcgagc aaggtttgct gggtcaagcg cctcaattgt gggatacgct ggttcagttc
     3481 gtagagaccg acctgacgct caacgacgtg ctgtatctcg cggacgtcgc cgcccggctc
     3541 gataccgcgc acatccgcag ccggttcatc gacggctcca tgctcgcctg gttcgccacc
     3601 gaaaccggtg ccagcgtgct gcgctacgac tacagtgtgc tcgagccgta tctggaccag
     3661 acattcgcgc cgtttcctct aaatgcggcc tcacagaagc gagccgccgt ccgggtggtg
     3721 aatcgcgcgg gccatcctga ttgggagatc gtcgccgccg accgcctggc ctcggccggg
     3781 ttcaacgtcg cagagatgtc cgccgagaac gccgtcgccc cgcacaccac gatcgtcgac
     3841 tacacactca cccccaaggg atcgcgcctg aacgacctga tcgagtcgct gcaggtcgcc
     3901 ccagaaagcg ccgccagccg gcccgccgag aacagccggg tcgattacga agtgatcctc
     3961 ggcgcggact tcgagccgtg ccaacgcccc tcgcgcggtt ggtgatccat ttcttccccc
     4021 tgtcgccggg ccagtgccaa tcactctaac tctaacctct aactctaacc ctaactccta
     4081 actctaactc taactcccaa ctctaactcc gtaggcttgt cgccgtctat ccgtgcagac
     4141 ctcgtatttg acgactgccc cgcatcgcct ataatggcac tgtagggatt tttgcacggc
     4201 gcgaaaggac tcacatgggc acccgcgcgg c
//
LOCUS       DBNHXB010000375         9485 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1448742, whole genome shotgun sequence.
ACCESSION   DBNHXB010000375 DBNHXB010000000
VERSION     DBNHXB010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9485)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9485
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1448742"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(527..1078)
                     /gene="rsmD"
                     /locus_tag="AC162G_15200"
     CDS             complement(527..1078)
                     /gene="rsmD"
                     /locus_tag="AC162G_15200"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR5077200.1"
                     /translation="MRVISGTAKGRKLKAVPGDTTRPVTDRVKEAVFGIIGANVRDAR
                     FLDLFAGTGSVGIEALSRGAREAVFVEKERLALDAIRFNLEHTRLSARARVVRSDVFG
                     FLRGNPQAFEYIYVAPPQYRDLWLAVLGALDARPGWLADDGEIVVQIDPREYQETALE
                     HFEPIDRRRYGTTLVVFYARKTQ"
     gene            1296..3467
                     /gene="relA"
                     /locus_tag="AC162G_15205"
     CDS             1296..3467
                     /gene="relA"
                     /locus_tag="AC162G_15205"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HIR5077201.1"
                     /translation="MTKHPVLNIQSILDTLPDGNGSNGRLVVERAYAVAEHAHRAQRR
                     ASGEPYVQHCLAVAKILADLSLDPPAIAAGLLHDVLEDTEVSEEQMRRDFGDEIARLV
                     DGVTKLRQFDRMERRDIQSYNDHELESLRKMFLAMADDPRVMMIKLADRVHNMRTLGA
                     LPEEKRKRIASETLEVFAPLASRLGIWQFKWELEDLGFRHLNPDKYKEIARLIDERRG
                     DREASVNQIIQQLQERMKAESLPAEISGRPKHIYSIWRKMERKGVTFEQVHDARAVRV
                     IVDTVAQCYQTLGIVHSIWKPIPGEFDDYIATPKDNMYQSLHTAVVGGDGRPLEVQIR
                     TREMHESAELGIAAHWRYKEGGRRDPKYEEKIKWFRSLMDWRQDVSDAREFLDTLQTD
                     VFQDRVYAFTPKGKLIDLPAGSTPIDFAYHVHTDVGDRCRGARVNGKLVALDYPLQTG
                     DQVEILTAKRGGPSRDWINPNLGYTKTQRARTKIKQWFKKQDRAANIAQGREILEREM
                     RRLGFEKTALEHVASLFEMEKLDDLLEKIGAGDITSHQIATKLIEAEQQKAGEAQDVT
                     PEEVTSAPRRQTPVEFTVRGAGGLMTSLAQCCHPVPGDPIIGYITRGRGVRVHRRDCA
                     NILRGDEEERLIEVSWGSAEATYPVAIKISAYDRGGLLRDIAAVVSGEGINMTSVNVS
                     TSKQMATLVVTLEITGVAQLSRVFDKIERLPNVIEVRRQTN"
     gene            3467..4105
                     /locus_tag="AC162G_15210"
     CDS             3467..4105
                     /locus_tag="AC162G_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-alpha-glucoside-1,2-lyase-like
                     domain-containing protein"
                     /protein_id="HIR5077202.1"
                     /translation="MRTTIVHIGLLAALLVSACESAAPASDTPFADDFSDPGSGWSTT
                     NTEQLSISYADGALHFRVDAPDTAAWAVAGKRAGNFILDVDATQVEGPDNNHYGVIVR
                     YADNRNFYRLDISGDGYYSIQRYRDGEWETLIDWRESEAIRQGATTNHLQVIAEGPKL
                     TWIVNGAAVDSIEDTDILVGDVGLTAGAFPDESGVHIAFDNFQVRPLETTEP"
     gene            complement(4136..4288)
                     /locus_tag="AC162G_15215"
     CDS             complement(4136..4288)
                     /locus_tag="AC162G_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077203.1"
                     /translation="MAALIGRDEVQHAILWTQMNADFATVIIVPNEPIGGGQGGVRTQ
                     KMPGET"
     gene            4373..6652
                     /locus_tag="AC162G_15220"
     CDS             4373..6652
                     /locus_tag="AC162G_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5077204.1"
                     /translation="MTRSEFEWQIAEEVGAAPGPVAGTPVKLGRRAWLVALAVVAAMA
                     IAGTALLVAANRRTEEAIAGIKMDVLASHALAQRAANLTDRDLLDVVLDESPGWRATQ
                     HELLDRRLITGRDPFGFTAQPVTPEVIEAILSSDLKQAQVVSERAYAVAGIGSDAETV
                     HLRHTFVFRFDGQRWRWSPLSDEGFWGPPGTIRGQRLAWLYPMHDVETVKRLARDAEA
                     ALGRICARMGSAMCPDDAALTITLATDPSSLIMMAAGEPARAAILGDNATLTLPAISL
                     FGTPADEASYQALYRLYTTRIAAALAGQSARAARSQGAVAGLFYQAVNRAMIEEGLRP
                     WPLRASGDPSLSPVSLPDQEIALYCVEDVQRGGDLRRYDPSTALRAGPATGSWHEELS
                     DRIFTSLTPLPDDGGVILQEQQEVAGQPWSRIILWRDQKQLTVFDRPPGFVLDYSLGW
                     VSSQSHNWLRFTVGPDRAARDAHFVDHRLLDLDSCRAPDSAYCESVSVTGYSYPVWSP
                     DGSRSIVMWTPDRAPVLSPYVWKENTLLFQGSGEGRPLKFIGMGFAPFWLDDTTYGYI
                     SPGRYSPTEVFTATVAGDGSHVVSWADDVRSALPSNERTRQIGIQRVVVNPVDSQWLL
                     IETSSAGLGDQDDHAFFFNRRFRDARRLPFAPAYATYDFSPDGRWLVVRSFDPKTAAW
                     PVYVHDIEQNQTQTFTYDDPYWADPATPPPIDWSADGRWLLVLHDGVVHLIAPDHDYR
                     RIIVPESPGCVFAAWINRR"
     gene            6683..8887
                     /locus_tag="AC162G_15225"
     CDS             6683..8887
                     /locus_tag="AC162G_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077205.1"
                     /translation="MSDPQFDWRIEEEKVRDRAPGALSPTDRSAQVSSSGHAVRRTAL
                     IAVPLLALAIVAVYLGVVRRAEVAMEDVQKDVLAVHALMQRAVEQLDADLLVTVLPED
                     DPAWKNTVLMLLGRGLLFDRATFNLSALPAGPKADKVTLSPDLTAAQVAAERAYATSA
                     AHGLTQTIRLQHTVVYHFDGQRWRFAEPDDEFWGRPITTTGAILTLAYPERDKALGQS
                     LARDLDAVLSQTCLELYSDACAGDRRMRLTLSGNPLSLIQMSEPDGIFTTVRSGVIDF
                     ALPAPSLFGVPLDEPSYQALYRTYAARLVDVFADDVLGLRRRDSLAWFRQGLRDNLMV
                     RLGLQPWPGIAVSEPAVSPPGPRPEQDIAVYCVDGLQRYGSLYRYSSSTDAWSTVSSG
                     RLLAAMAPLPGGEGLLLQESMTADGAASRLILWQDGREKILLDALSTLDLVVPNVQIV
                     PPGHKLIVRWQSVDIAGERADYLLIDLKRCGSRGCETSTLSDLPVWSPDGSHTLVLSS
                     RDSRLLLGNEDGQALIDLGGGIDPFWISDDTYGYFTDPFGRAPAVVTATVANSETHPL
                     LSLAGLVPEEAEQLGLWQIIPGQAAGSNMVIAVYTLPPQEARRTYWFLIDGRSGDTKP
                     LPLEDEFFGLGLSPDERWLVGFTFDAAARALSLQFFDLHDFQASQLKTPAPESAYGAL
                     ALAYAGENWSSDGQWFLSLNDDTLYLIAPGYDYYRAIAPESPGCIFAAWINR"
     gene            8905..>9485
                     /locus_tag="AC162G_15230"
     CDS             8905..>9485
                     /locus_tag="AC162G_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077206.1"
                     /translation="MPRVDFDWEIGEGEPENLPPQGGDQSDAPAPKPGAAVERPAWPR
                     AQTKRVVLIVACVLFLTGAIATAIVARHVEDVTAPLIKDVLAAHNLVRHAVTQSDEDL
                     FDSLLYRGDAGWRTAYRQLFRQDALIDRAPLGLHAQADDARIAGVTLSPDMTGAEVVS
                     EHAYAIDAGGGLTETVRLRQIAIYRFEKGQWLLA"
BASE COUNT         1734 a         3097 c         3028 g         1626 t
ORIGIN      
        1 cgtggctgtg tcgacggcgg cgaccacgat gggctgcggc gatcggccac acccgacgac
       61 gccgatcgcg agcgcgagaa gaatcaggac acggatgaac gcggataaac gcggattgac
      121 cctcgcagcg aagcggaggg gattggaact acatcgcatg gtcactactc ggctacttgc
      181 agcgtgtcat tccgaacgca gtgaggaatc tctgctccga cgggcgggcg cagcgtgtcg
      241 gggtagagat gcctcgtcgt gcctcctcgg catgacacaa cgtgcctcct cgcatgacac
      301 aatccgcgcc catcgcccca gcgtgtcatt ccgaacgcag tgaggaatct ctctcccggc
      361 cggtgcgcac cccatgccgg ggtagagatg cctcgtcgta cctcctcggc atgacacaat
      421 ccgcacgcag tgaggaatct ctgctccaac cggcgggcgc ggcgtgccgg ggtagagatg
      481 cctcgtcgtg cctcctcggc atgacacaac gtgcctcctc gaagaactac tgagtcttgc
      541 gcgcgtagaa gacgacgagc gtggtgccat agcggcggcg atcgatcggc tcgaagtgct
      601 ccaacgccgt ctcctggtat tcgcgcgggt cgatctgcac gacgatctcg ccgtcatcgg
      661 ccagccagcc aggccgagcg tcgagcgcgc caagcacggc cagccacaag tcccggtact
      721 gcggcggcgc gacgtagata tactcgaacg cctgcggatt cccacgcaga aacccgaaca
      781 catcggagcg cacgacacgc gcgcgcgccg aaagccgcgt gtgctcgagg ttgaagcgaa
      841 tcgcatcgag cgcgagccgc tctttttcga cgaagacggc ttcgcgcgcc ccccggctaa
      901 gcgcctcgat gccgacgctg cccgttccgg cgaacaggtc gaggaagcgc gcgtcccgca
      961 cgttcgcgcc gatgatgccg aacacggcct ccttgacgcg atcggtgacg gggcgcgtcg
     1021 tatcgccggg cacggctttc agcttacggc ctttggcggt tccggaaatg acgcgcatgt
     1081 tgcagatggc ttttcgcttg tcgcttgtgg catatcgctg atcgctacac cgcgacttgg
     1141 cggcgcgagt cagcgtgtgc ctgccgcgta cgaatcgtac cacaaagctt gcatcaacgc
     1201 atgcggcggc cagggcacat ccatgtacgc gatccgttgc ctatcctttg accattcacc
     1261 cgctccgcgc tatagttatg tagcggaatc ggagtatgac gaagcaccct gtcctcaaca
     1321 tccaatccat tctcgacacg ctgccggacg ggaacggcag caacgggcgc ctggtcgtgg
     1381 agcgcgccta cgccgtggcc gagcacgcgc accgcgctca gaggcgcgcc tcgggcgagc
     1441 cttacgtgca gcactgcctg gcggttgcca agatcctggc ggatctcagc ctcgacccgc
     1501 ccgccatcgc cgccggcctg ctgcacgatg tcctcgaaga cacggaggtc agcgaggagc
     1561 aaatgcggcg cgacttcggc gacgagatcg cacggctcgt ggacggggtc acgaagcttc
     1621 gccagttcga ccggatggag cggcgcgaca ttcagagcta caacgaccac gagctggagt
     1681 cgctgcgcaa gatgttcctg gcgatggccg acgatccgcg cgtgatgatg atcaagctgg
     1741 ccgaccgcgt gcacaacatg cgcacgctcg gcgcgctgcc ggaggagaag cgcaagcgca
     1801 tcgcgagcga gacgctggag gtcttcgcgc cgctggccag ccggctcggc atctggcagt
     1861 tcaagtggga gctggaagac ctcggcttcc gccacctcaa cccggacaag tataaggaga
     1921 ttgcccggct gatcgacgag cgccgtggcg atcgcgaggc gagtgtcaac cagatcatcc
     1981 aacagcttca ggagcgcatg aaggcggaga gcctcccggc cgagatctcc gggcgcccga
     2041 agcacatcta cagcatctgg cgcaagatgg agcgcaaggg cgtgacgttc gagcaggtgc
     2101 acgacgcgcg cgcggtgcgc gtgatcgtcg acacggtggc gcaatgctac cagacgctgg
     2161 gcatcgtgca ctcgatctgg aagcccatcc ccggcgagtt cgacgattac atcgcgacgc
     2221 cgaaggacaa catgtatcaa agcctgcaca cggccgtcgt gggcggcgac gggcgcccgc
     2281 tcgaagtgca gattcgcaca cgcgagatgc acgaatcggc cgagctgggc atcgccgcgc
     2341 actggcgcta caaggaaggc gggcggcgcg atccgaagta cgaagagaag atcaagtggt
     2401 tccgctcgct gatggactgg cggcaagacg tctccgacgc gcgggagttc ttggacacgc
     2461 tgcagaccga cgtgttccag gatcgggtgt acgccttcac gccgaagggc aaactgatcg
     2521 acctgcccgc cggctcgacg ccgatcgact tcgcctacca cgtgcacacc gacgtcggcg
     2581 atcgctgccg gggcgcacgc gtcaacggca agctggtcgc gctggactat ccgctccaga
     2641 ccggggacca ggtggagatc ctcacggcca agcggggcgg ccccagccgg gactggatca
     2701 accctaatct gggctacacg aagacccagc gcgcccgcac caagatcaag cagtggttca
     2761 agaagcagga ccgcgccgcg aatatcgccc agggacgcga aatcctggag cgcgagatgc
     2821 gccggctagg cttcgagaag acggcgctcg agcacgtggc gagcctgttc gagatggaga
     2881 agctcgacga cttgctggag aagatcggcg cgggcgacat cacctcgcac cagatcgcga
     2941 cgaaactgat cgaggccgag cagcaaaagg ccggcgaggc gcaggacgtc acgccggaag
     3001 aggtgacctc cgcgcctcgc cggcagacgc cggtcgagtt cacggtgcgc ggcgccggcg
     3061 gcctgatgac ctcgctggcg cagtgttgcc acccggtgcc cggcgatccg atcatcggct
     3121 acattacgcg cggacgaggt gtgcgggtgc accggcgcga ttgcgccaac attttgcgcg
     3181 gcgacgaaga agagcggctc atcgaggtct cgtgggggag cgcggaggcg acgtacccgg
     3241 tcgcgatcaa gatttctgcc tacgatcgcg gcgggctgct gcgcgacatc gcggccgtcg
     3301 tgtccggcga aggcattaac atgacgagcg tcaacgtcag caccagcaag cagatggcga
     3361 cgctggtcgt gacactggaa atcaccggcg tggcgcagct ctcgcgcgtg ttcgacaaga
     3421 tcgagaggct gccgaacgtc atcgaggtgc ggaggcagac gaactagtgc gcactaccat
     3481 cgttcatatc ggtttacttg ccgcactgct cgtatctgcc tgtgaatcgg ctgcgcctgc
     3541 cagcgacacg ccgttcgcgg atgatttcag cgaccccggc agcggctggt cgacaacgaa
     3601 cacggagcaa ctgagcatct cctacgcgga tggcgcgctg catttccgcg tcgacgcgcc
     3661 cgacaccgcc gcgtgggcgg tggccggcaa gcgcgccggc aacttcatcc tggacgtcga
     3721 cgcgacgcaa gtggaaggtc ccgacaacaa tcattacggc gtgatcgtgc gctacgccga
     3781 caacaggaac ttttaccggc tggacatttc cggcgacggt tattacagca tccagcgcta
     3841 tcgcgacggc gagtgggaga cgttgatcga ctggcgggag tcggaggcga tccggcaagg
     3901 cgcgacgacg aaccatcttc aggtgattgc cgaggggccg aagcttacgt ggatcgtcaa
     3961 cggcgccgcc gtcgattcca tcgaagacac cgacatcctg gtcggcgacg tgggcctgac
     4021 cgcaggcgca ttccccgacg aatccggcgt ccacatcgcg ttcgacaact tccaggtcag
     4081 gccgctggag acgaccgagc cgtgacccgg cgaatggcgg ctatggagag ccgccctacg
     4141 tctcgcctgg catcttttgc gtgcgcacac ccccctgccc tcccccgatc ggctcgttcg
     4201 gcactataat cactgtagcg aaatccgcgt tcatctgcgt ccacaagatg gcgtgctgca
     4261 cctcatcgcg cccgatcaag gcagccacac ggcgatcgtg cctggatcgc ccgggtgcgc
     4321 gttcgcagcg tagatcgatc gctgagccga gccgcatgcg gagaacgagg tcatgacccg
     4381 ctccgaattc gagtggcaaa tcgccgagga ggtaggggct gcccccggcc cggtcgccgg
     4441 aactcccgtc aagctcggga gaagggcttg gctcgtcgcc ctcgccgtcg ttgccgccat
     4501 ggccatagcg ggcactgcct tgctcgtcgc agccaaccgg cggacagaag aagcgatcgc
     4561 cggaataaag atggatgtcc tcgccagtca cgcgctggcg cagcgcgcgg cgaacctgac
     4621 cgatcgggat ctgctcgatg tggtgctgga cgagtccccc ggctggaggg caacccaaca
     4681 tgaactactc gatcggcgac tgatcacagg tcgggaccct ttcgggttca ccgcgcagcc
     4741 ggtcacgccc gaagtcatcg aagccattct ctcttctgat ctgaaacaag cgcaagtcgt
     4801 atctgagcga gcgtatgcgg tcgccggcat aggcagtgac gccgagacgg tgcaccttcg
     4861 gcacactttc gtcttccgct tcgatgggca acgctggcgg tggtctcctc tctctgacga
     4921 gggcttctgg gggccgccag gcacaatcag aggacaaagg ctcgcgtggc tgtatcccat
     4981 gcacgacgtc gagacggtta agcgattggc gcgcgatgcg gaagcagcgc tcggccggat
     5041 atgcgctcgc atgggcagcg cgatgtgccc ggatgacgcc gcgctgacca tcaccctggc
     5101 gaccgatccg tcgagcctga tcatgatggc tgcaggcgag ccagctcgcg ccgccattct
     5161 gggcgacaac gcgacgctca ccctgccggc gatctcgctg ttcggcacgc cggcagacga
     5221 agcgagttac caggcgctgt atcgtctgta cacgacgcgc attgccgccg cgctggcagg
     5281 ccagtccgcg cgggccgcgc gcagccaggg cgcagtcgcc gggctgttct atcaggcggt
     5341 gaacagagcg atgatcgagg aagggctgcg accctggccc ctccgcgcaa gcggcgatcc
     5401 gtcactttca cctgtttcct tacctgacca ggagattgcg ctgtactgcg tggaagacgt
     5461 gcaacggggc ggcgaccttc gccgatatga cccttcgact gcgctcaggg caggccccgc
     5521 gaccggctca tggcacgaag agctgtccga ccgcatcttc acgtctctca cgccgctgcc
     5581 ggacgacggc ggagtcatct tgcaggaaca gcaggaagtg gccggccagc cgtggtcgcg
     5641 catcatcctt tggcgagatc agaaacaatt gacggtgttc gacaggccgc cgggcttcgt
     5701 gctcgattat tctctcggct gggtcagctc gcaatcgcac aactggctgc ggttcaccgt
     5761 cggtcccgat cgagcggcgc gcgacgccca tttcgtagac caccgcctgc tggacctgga
     5821 tagctgccgg gcgccggatt cggcgtattg cgagtcggtc agcgtcaccg gctatagcta
     5881 tccggtctgg tcgccggatg gatcgcgcag catcgtgatg tggacgccgg atcgcgcgcc
     5941 cgttctgtca ccctacgtgt ggaaggagaa cacgctgcta tttcagggaa gtggcgaagg
     6001 ccggccgctg aagttcatcg gtatgggctt cgcacccttc tggctggacg acacgaccta
     6061 cggctacatc tcgcccggcc ggtactcacc taccgaagta ttcacggcga cggtcgccgg
     6121 cgacggcagt cacgtcgtat cgtgggcaga cgacgtccgg agcgcgttgc ccagcaacga
     6181 gcggacacgg caaatcggca ttcagcgcgt cgtcgtcaac ccggttgatt ctcagtggct
     6241 cctgatcgag acttcgagcg ccggtctagg cgaccaggac gatcacgcat tcttcttcaa
     6301 tcggcgcttc cgcgatgcgc gacgcctgcc gttcgccccg gcctatgcca cgtatgattt
     6361 ttcgccggac ggccgctggc tggtcgtccg cagtttcgat ccgaagacgg ctgcctggcc
     6421 tgtttatgtg cacgacatcg agcagaatca gacacagaca ttcacttacg acgatcccta
     6481 ctgggccgat ccggccaccc ctcctccgat cgattggtcg gccgacggcc gatggctgct
     6541 ggtccttcat gacggcgtgg tgcacctaat cgcgcccgac cacgactacc gacggatcat
     6601 cgtcccggaa tcgcccggtt gcgtgttcgc cgcgtggata aaccgccgtt gacacttcgg
     6661 cctgccattt tgagccacgc gcatgtcaga cccgcagttc gactggcgaa tcgaagaaga
     6721 gaaagttcga gaccgcgcgc cgggcgccct ttcaccgacg gatcgaagcg ctcaggtctc
     6781 gtcttctggc catgccgtgc ggcgaactgc gctcatcgcc gtgccgcttc tggcgctcgc
     6841 cattgtagcc gtgtatctgg gcgtcgtgcg gcgcgccgag gtagcgatgg aagatgtgca
     6901 aaaggatgtc ctcgccgtgc acgcactgat gcagcgcgcg gtcgagcagc tcgacgcaga
     6961 cctgctcgtc accgtgttac ctgaggacga tccggcgtgg aagaataccg tcctgatgct
     7021 gcttggacgg gggctgctct tcgacagagc gacgtttaac ctgtccgcgc tgccggccgg
     7081 cccgaaggcg gacaaggtca ctctctcccc cgacctgacg gcagcccaag tggcggcgga
     7141 gcgtgcgtac gccaccagcg ccgcgcacgg cctgacgcag acgatacgcc tgcagcacac
     7201 cgttgtctac cacttcgacg ggcagcgctg gcgattcgcc gaacccgatg acgagttctg
     7261 gggcaggcca atcacgacga caggcgcaat cctgacgctc gcttaccccg agcgcgacaa
     7321 agcactcggc caaagcctgg cgcgtgacct ggacgcggtg ctgagccaga catgcctcga
     7381 attgtattcc gatgcctgcg ctggcgatcg gcgcatgcgg ctgaccctct ccggcaatcc
     7441 gttgagtctc atacagatga gtgaaccaga cggtattttc actacggttc gcagcggcgt
     7501 aatcgatttt gcactgccgg ctcccagcct gttcggagtt ccgctcgacg aaccgagcta
     7561 ccaggcgctg tatcgtacct atgctgcgcg gctggtggac gtcttcgccg acgacgtgct
     7621 tggccttcgg cggcgtgaca gcctggcctg gttccggcag ggcctgcgtg acaacctgat
     7681 ggtccggctc ggcctgcagc catggccggg gatagccgtg agcgagccgg cagtttcccc
     7741 gcccggcccg cggcccgagc aagacatcgc cgtctactgc gtcgacggcc tgcagcgcta
     7801 tgggagcctg tatcgctatt cctcttcgac ggatgcatgg tccacagtgt cctccggccg
     7861 cctgctggcc gcaatggcgc cactgccagg aggcgaaggt ctcctgctcc aggagtccat
     7921 gactgctgat ggcgccgcgt cgcgcctgat actctggcaa gatgggcggg aaaagattct
     7981 gctcgatgca ctcagtaccc tcgacctggt tgtcccgaat gttcagatcg ttcccccggg
     8041 ccacaagctg attgtccgct ggcagagtgt cgacatcgcc ggtgagcgcg ccgattacct
     8101 tctgattgac ctgaagcggt gcggctcacg gggatgtgaa acaagcacct tgtccgatct
     8161 gcctgtctgg tcacctgacg gctcgcacac gcttgtgctc agttcgcgag atagccggtt
     8221 gctgctgggg aatgaagacg ggcaagcgct gatcgacctc ggcgggggta tcgatccatt
     8281 ctggatcagc gacgacactt acggctactt cacagacccg ttcggccggg cgccggcagt
     8341 cgtcacggcc accgtagcga acagcgagac gcacccgctg ctgagccttg cgggtctggt
     8401 accggaggaa gccgaacagc taggcctgtg gcagatcatc cccggccagg ccgccggcag
     8461 caacatggtg atcgccgtct acactctccc gccacaagag gcacgccgca cgtactggtt
     8521 cctgatcgat gggcgttctg gtgacacaaa gccgctgccg ctcgaggacg agttcttcgg
     8581 ccttggcctg tcaccggatg agcgctggct ggtcggcttc acgtttgacg ctgcggcacg
     8641 cgcgctctcg cttcagttct tcgacctgca cgacttccag gccagccagt tgaaaacgcc
     8701 cgcgcctgag agcgcttacg gggccctggc gcttgcctac gcaggcgaaa actggtcatc
     8761 cgatgggcaa tggttcctca gcctcaacga cgacaccctc tacctcatcg cgccgggtta
     8821 cgactattat cgcgccatcg cccccgaatc gcctggctgt atcttcgcgg cctggatcaa
     8881 ccgctgaggc aggaggcgca ccccatgcca cgtgtagatt tcgactggga aatcggcgaa
     8941 ggcgagccgg agaacctccc acctcagggc ggcgatcaaa gcgacgcgcc cgccccgaaa
     9001 cccggcgcgg cggtcgagcg cccggcgtgg ccccgggcgc agacgaagcg tgtcgtgctt
     9061 atcgtcgcgt gcgtgctctt cctgaccggc gccatcgcga ctgcgatagt cgcccgtcac
     9121 gtcgaggacg tgaccgcgcc tctcatcaag gacgttctgg ctgctcacaa cctggtgcgc
     9181 catgccgtca cgcaatccga cgaagacctg ttcgattccc tgctatatcg cggcgatgcc
     9241 ggttggcgca cagcttaccg gcagcttttc aggcaggacg cgctgatcga ccgcgcacct
     9301 ctgggtctgc acgcgcaggc ggacgatgcc cggatcgccg gcgtgacact ctcaccggat
     9361 atgacggggg cggaagtcgt ctccgagcac gcctacgcca tcgatgccgg cggcggcctg
     9421 accgagacgg ttcgcctgcg ccagatcgcc atctaccgct tcgagaaagg acagtggctg
     9481 ctggc
//
LOCUS       DBNHXB010000376         2591 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1951707, whole genome shotgun sequence.
ACCESSION   DBNHXB010000376 DBNHXB010000000
VERSION     DBNHXB010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1951707"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="AC162G_15235"
     CDS             <1..407
                     /locus_tag="AC162G_15235"
                     /inference="COORDINATES: protein motif:HMM:NF024799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR5077207.1"
                     /translation="RADNIEKCLKEKAPNVNVVGRFLGGTTENGLKSMETALQANPDI
                     DVVVSINDAGAYGALTALEAAGKDPATTIVVGIDAEQQAKDLIKEGGYYRGTVDTAPA
                     TTGEMVINAAVKILSSATVAKNIRVPVEKVTE"
     gene            538..>2591
                     /locus_tag="AC162G_15240"
     CDS             538..>2591
                     /locus_tag="AC162G_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077208.1"
                     /translation="MKARSRLPFDRWIEMDLQWFHPDRLGEQIPVLLDRLAPLYAPIA
                     GMRGLVFNVGWLIDLVTEWSGRVDQPLPLHSRRTAGWNDRTYGDLRSFFARLKQEAAR
                     RDLGDLKIGVLFVNWGHVSWPPDLKIYDFDSDWYDRHPEVYGEPHSFIGMPELHPGRL
                     LHADSYPYATYPNGVSEGTPFIDLLGPQWGSVSRALGLDAIVLRDGFMGPMIYTRTGP
                     FGHTAPADPGEVRRWTDEVRRLFRTVKDANPDALVIGYSSAVSAVADWRVGCVDFESV
                     VADGAIDAWIDQTWGGAWQDWWHQEWKGWTFQLAYLLLHAVMIRAANRRRAAPCKHYN
                     LIETWDAWEPWDTLHQVPGKLRWAIWAFSHAATIDETGAPRTPDGSYISWANNRAGEL
                     LSEADIACVKSNLDAAQVSAARLERVYGPAAVYHRPMMEWLSAEHPDWNVSEWIDEQI
                     GFLMKWGVPILSATRSEWARGAQPEALVLQTPGHFLESEQRAWLDTLRTKPALMIGRA
                     DVIDPALLERAGARARGDLSPQGFVRASIDPSPLKGDLPDFNVLHLPPHQPIVGEGEV
                     LVRAGSTPALVRRDNVIYWQPPDWSEPSNPFLPRYQIGSLATHALAARALVDACARAG
                     LSHAADVPFAQPVAFHLWRSDGRIHILLGNLETGLTGDARTGRRVTLKLERRHLDLDA
                     GD"
BASE COUNT          467 a          834 c          853 g          437 t
ORIGIN      
        1 tgcgcgccga caacatcgag aagtgcctga aggagaaagc gccaaatgtg aacgtcgtcg
       61 gccgcttcct gggtggcacg accgagaatg gactcaaatc gatggagacc gcgctgcagg
      121 ccaaccctga catcgacgtc gtcgtcagca tcaacgatgc cggcgcgtat ggcgcgctga
      181 cggcgctgga ggccgccggc aaagaccccg cgacgacgat cgtcgtcggc atcgacgccg
      241 aacagcaggc caaggacctg atcaaggaag gcggctacta ccgcggcacg gtcgacaccg
      301 caccggccac gaccggcgag atggtgatca acgccgctgt aaagatcctc tccagcgcca
      361 ccgtggccaa gaacatccgc gtcccggtcg agaaagtgac cgaataggtt ccgaagtaga
      421 cgtgaaccgt gacgagtgat gcgtgttgcg tgttgcgtga tgcatgataa gtcacgcatc
      481 acgcatcacg tatcacgttt tacgtatcac gctcaccctg ttgacgacca atatcccatg
      541 aaagcacgct cccgtctccc cttcgatcgc tggatcgaga tggatttgca atggttccat
      601 cccgatcgac tcggcgagca gattcccgtg ctgctcgatc ggctggcgcc gctgtatgcg
      661 ccaattgcgg ggatgcgcgg gctggtcttc aacgtcggct ggttgatcga cctggtcacc
      721 gaatggagcg gccgggtcga tcaacctttg ccgctgcaca gccgccgcac ggcgggctgg
      781 aacgatcgga cgtacggcga tctacgttcc ttcttcgctc gtttgaagca ggaagccgcc
      841 cggcgcgacc tgggcgatct caagatcggc gtattgttcg tgaactgggg ccacgtgtca
      901 tggcccccgg acctcaagat ctacgacttc gacagtgact ggtacgatcg ccatcccgaa
      961 gtctatggcg agccgcactc atttatcggc atgcccgagc tgcatccggg ccgcctgctg
     1021 cacgccgact cgtacccgta tgcaacgtat ccgaacggtg tcagcgaagg cacgcctttc
     1081 atcgatctgc ttggcccgca gtggggatcg gtctcgcgcg cgctggggct tgacgcgatc
     1141 gtgctgcgcg acggcttcat ggggccgatg atctatacgc gcaccggccc gttcggccac
     1201 acggcgccag ccgatccggg tgaggtgcgc cgctggacgg acgaggtgcg gcggctgttc
     1261 cgcaccgtga aagatgctaa ccccgacgcg ctcgtcatcg gctactcgtc ggcggtgagc
     1321 gcggtcgccg actggcgcgt cggctgtgtc gacttcgaga gcgtcgtcgc cgacggcgca
     1381 atcgacgcct ggatcgacca gacgtggggc ggcgcctggc aggactggtg gcatcaggaa
     1441 tggaagggct ggaccttcca gttggcctac ctgctcctgc acgcggtgat gatccgcgcg
     1501 gcgaaccgcc gtcgcgcggc gccgtgcaag cactacaacc tgatcgagac gtgggatgcg
     1561 tgggagccgt gggacacact gcatcaggtt ccgggcaagc tgcgctgggc aatatgggcc
     1621 ttctcgcatg cagccacgat cgacgaaacc ggcgcgccgc gcacgcccga cggctcgtac
     1681 atctcgtggg cgaacaatcg cgcgggcgag ctgctcagcg aagccgacat cgcgtgcgtg
     1741 aagagcaacc tggatgcggc ccaggtcagc gcggcccggc tcgaacgcgt ctacgggccg
     1801 gcagcggtgt atcaccgccc gatgatggaa tggctctcgg cagaacatcc ggattggaac
     1861 gtgagcgagt ggatcgacga gcagatcggg ttcctgatga aatggggcgt gcccatctta
     1921 tcggcgaccc gcagcgagtg ggcgcggggg gcacagccgg aggctctggt cttgcagaca
     1981 ccaggccact tcctcgaatc ggagcagcga gcttggctgg acacattgcg cacaaagccg
     2041 gcgctcatga tcgggcgcgc cgacgtgatc gacccggcat tgctcgagcg cgcgggtgcg
     2101 cgcgcccgcg gagacctgtc gccgcaggga ttcgtgcgcg ccagcattga cccatcgccg
     2161 ctcaagggcg acctgccgga cttcaacgtg ctgcacttgc cgccgcacca gccgatcgtg
     2221 ggcgaaggcg aggtcctggt tcgcgccggg tcgacgccgg cgctcgtccg gcgcgataac
     2281 gtgatttact ggcagccgcc cgactggagc gagccgtcga acccgttcct tccgcgctac
     2341 cagatcggca gcctcgccac gcacgcgctg gctgcccgcg cgctggtgga cgcctgtgcg
     2401 agagccggac tcagccacgc cgccgacgtg ccattcgcac agcctgtcgc gtttcacctg
     2461 tggcgcagtg atggccgaat tcacattcta ctgggcaatc tcgaaaccgg cctgaccggc
     2521 gacgcacgca cggggcgccg cgtgacgctt aagctcgagc ggcggcatct ggaccttgac
     2581 gcgggcgatt a
//
LOCUS       DBNHXB010000377         5581 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2120714, whole genome shotgun sequence.
ACCESSION   DBNHXB010000377 DBNHXB010000000
VERSION     DBNHXB010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2120714"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="AC162G_15245"
     CDS             complement(<1..568)
                     /locus_tag="AC162G_15245"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HIR5077209.1"
                     /translation="MATDIVLFIILAAIAIVSAIAMIVTRNAVHSALFLVMVMVVLAI
                     FFLGLGGPFIAMVQVTVYAGAIMVLFLFVVMLLGAERVGAQSGLKWQVPVALLLGFFL
                     LVAAGVLLFSAQATAVAVEPQPAASFDTVIPEACAEDVALVQTSGVVQGTPCLVGDSL
                     FTTYLFPFEVVSILLLAAMVGAVVLTRRP"
     gene            complement(640..1149)
                     /gene="nuoI"
                     /locus_tag="AC162G_15250"
     CDS             complement(640..1149)
                     /gene="nuoI"
                     /locus_tag="AC162G_15250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HIR5077210.1"
                     /translation="MIFELFKGFKTTLKEFITPPVTIQYPEVTRPVRQRFRGRHELRR
                     YNDGLERCIGCALCAAACPADAIYVEAAENTDEERYSPGERYAKTYEINMLRCIFCGY
                     CEDACPTDAIVLTNTYELSSYDRKGLIYTKDMLIVPPPEGKPGTPQTVSDPEKRTRSI
                     LPEGLVNEP"
     gene            complement(1203..2279)
                     /gene="nuoH"
                     /locus_tag="AC162G_15255"
     CDS             complement(1203..2279)
                     /gene="nuoH"
                     /locus_tag="AC162G_15255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HIR5077211.1"
                     /translation="MDAVTQAIVWIVQILVIVFALLTGFAYTTLLERKVVARYQARIG
                     PNRAGYIPLPGGGRILGGWLQPLADGIKLIFKEELIPAGADRVIFILAPIITVMPALV
                     LFAVMPFGPTNANLFGLPISLTLAPNLNVGVLYILAVTSISVYGVTLAGWSSNNKYAL
                     LGGIRAAAQMVSYELALGLSVVSVVLLAGTLDTNAIVDQQKNMWFVFLQPLAAVIFIM
                     ATFAETNRAPFDLLEADQELVAGFNTEYSGMKFALFYMAEYIKLIAVSGLLVTFFFGG
                     YREPFPPALTQALPWLSVDNVPWLGPIYFLGKIIVLLVVAVWVRATLPRFRYDKLMAF
                     GWKLMLPLALGNVVVTAVAILLRS"
     gene            complement(2282..4855)
                     /gene="nuoG"
                     /locus_tag="AC162G_15260"
     CDS             complement(2282..4855)
                     /gene="nuoG"
                     /locus_tag="AC162G_15260"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HIR5077212.1"
                     /translation="MADLVNLYIDDQLVSVPRGTVVVEAAKKMGTDIPVFCYHPKLEP
                     VGMCRMCLVEIGRPKLDPATKQVVVGPDGRPEIAWGIKLETGCTTPVSEGMRVRTKSD
                     KARAAQRDVLEFLLTSHPLDCPICDKGGECPLQNLTMGYGPGVSRFDFAGKQRLDKHF
                     PLGDLIFLDKERCIQCARCIRFQDEIADDRVIGFNERGRTLEIATLSEPGFYSKFSGN
                     TTDICPVGALTTSDFRFRARPWEMTNVPSICPHCAIGCNITLGTRRSNARRGHWDILR
                     VMPRQNEEVNEIWICDKGRFGHHYALSEERLVKPLIRKDGRLFVVSWDEALALVAARL
                     KAAGSNAGALIGDRLANEDVYLIRKLFSEGLQSARINARPHVAAHEIATRYGVGAGSD
                     FGKMGEGSAILVIAGDVEEEAPVWFLRIRGAVKRGAQLIVAHGYETKLDRYARLALRY
                     KLGAESYVAFGILNALLSENRGEGVEGFAEVKSRAAEYSPEKVEQLTGVPAPAIRDAA
                     QIIAEATDVVIIFGQMALNLVKTGGEMLPQASALAQACANIAVATGHFGRPNNGLLPL
                     WPHNNTQGTHDLIGDLRFGAEPMPEVLYMVGTDPVGEGHAPRRRGNDGPFRIVQELFL
                     TQTAQDADVVLPALSFAERDGTTTSADRRVQRFYRALPALGDGKADWEIAQDIAWRLD
                     LNWDYHTAEEVFAEIASAQPRYAGLSYAALAQSAPQWPPVGDKDLYFGGTAYDNRGGL
                     GAQTKSGVELDGRASLIWIDAPAQPDGFLVVPSRRLYDRGTLIDQSKPFQPRLRNATL
                     ELHADDAAELGVSDGDAVAVSVDGREVRMTAHVDGRAPQGAALAPAGVPGGAAQVRKG
                     A"
     gene            complement(5018..>5581)
                     /locus_tag="AC162G_15265"
     CDS             complement(5018..>5581)
                     /locus_tag="AC162G_15265"
                     /inference="COORDINATES: protein motif:HMM:NF022059.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HIR5077213.1"
                     /translation="FELGKITFRDLIFGAEYGQGLPELDRAVKAIMPSGASSTLLTED
                     ALDVRLDYESVAAAGSMLGSASVIIMDNTVDVAWAAHKVTRFFKHESCGKCTPCREGT
                     HWMLQTYEKIASGKGQKADVEQLGTVAGQMVGKCFCPLGEFATGPVLSSLKLFRGDYE
                     AKVDGAKAAAKPAAKPVAKPAPVKQAA"
BASE COUNT         1026 a         1902 c         1689 g          964 t
ORIGIN      
        1 tcgggcggcg cgtcagcacg accgcaccga ccatggccgc cagcaacagg atcgatacaa
       61 cttcgaaggg aaagagatag gtggtgaaga gcgaatcgcc gaccaggcac ggtgtgccct
      121 gcacgacgcc cgacgtctgg accagggcga cgtcctcggc gcatgcctcg ggtattaccg
      181 tatcgaaact cgccgccggc tgcggctcga cggccactgc cgtcgcctgt gcgctgaata
      241 gcagcacgcc ggccgcgacc agtagaaaga agccgagcag caacgccacc ggcacctgcc
      301 atttcaggcc gctctgtgcg ccgacccgct ccgcgccgag cagcatgacg acgaacagga
      361 acagcaccat gatcgcgccg gcgtagacgg tgacctgcac catggcgatg aacggcccac
      421 ccaggccgag gaagaagatc gccagcacga ccatcaccat caccaggaac agcgccgagt
      481 gcaccgcgtt gcgcgtgacg atcatcgcga tggcgctgac aatggcgatg gccgccagga
      541 tgatgaagag gacgatgtca gttgccacag gcactccggt gtgtacaaga gattagagat
      601 tggagattga gcgccaatct ctaatctcca atctctaaac tacggctcat tgaccagtcc
      661 ttccggcaaa atcgagcgcg tccgcttttc gggatcggac acggtctgcg gtgttcccgg
      721 tttgccttcc ggcggcggga caattaacat atccttcgtg tagatcaatc ccttgcgatc
      781 gtacgagcta agctcgtacg tattcgtcag gacgatcgcg tccgtcgggc aggcgtcctc
      841 gcagtagccg cagaagatgc agcgcagcat attgatctcg tacgtcttcg cataacgctc
      901 gccgggtgaa tagcgttcct cgtcggtgtt ctccgccgct tcgacgtaaa tcgcgtcggc
      961 gggacatgcg gccgcgcaca gcgcgcaccc gatgcagcgc tccagcccgt cgttgtagcg
     1021 gcgcagctcg tgccggcccc ggaatcgctg gcgcaccggc cgcgtgacct ccgggtattg
     1081 gatcgtcacc ggcggcgtga taaattcctt cagcgtcgtc ttgaacccct taaacagctc
     1141 aaagatcatg gcatcgcctc actccaactc cacactctaa ctccacactc taactctata
     1201 ctctaactcc gcaacaagat cgccaccgcc gtcaccacta cgttgccgag cgccagcggc
     1261 agcatcagct tccagccgaa cgccatgagc ttgtcgtagc gaaagcgcgg cagcgtcgcg
     1321 cgaacccaga ctgccacgac cagcaatacg atgatcttgc ccaagaagta aatcgggccg
     1381 agccacggta cgttatcgac gcttagccac ggcagcgctt gcgtcagggc gggtgggaac
     1441 ggctcccgat acccgccgaa gaagaaggtc accagcaggc cgctcaccgc gatcagcttg
     1501 atgtactcgg ccatgtagaa cagcgcaaac ttcatgccgc tgtactcggt gttgaacccg
     1561 gcgaccagct cctggtccgc ctcgagcagg tcaaacggcg cgcgattggt ctcggcgaag
     1621 gtcgccatga tgaaaatgac cgccgccagc ggctgcagga acacgaacca catgttcttc
     1681 tgctggtcga cgatggcgtt cgtgtcgagc gtccccgcca gcagcacgac gctcaccacc
     1741 gacaggccga gcgccagctc gtacgacacc atctgcgccg cggcgcggat gccgcccagc
     1801 agcgcgtact tgttgttgct gctccagccg gccagcgtca cgccgtacac gctgatcgac
     1861 gtgactgcca gaatgtacag cacgccgacg ttcaggttag gcgccagcgt cagcgagatc
     1921 ggcaggccga acaggttggc gttcgtcggt ccgaagggca tgaccgcgaa caacaccagc
     1981 gccggcatca ccgtgatgat cggcgccaga atgaatatga cgcgatccgc cccggccgga
     2041 atcaactcct ctttgaagat cagcttgatg ccatcggcca gcggctggag ccacccgccc
     2101 agaatgcggc cgccgcccgg cagcggaatg tatccggcgc gattcgggcc gatgcgcgcc
     2161 tggtagcgcg ccaccacctt gcgctccagc agtgtcgtgt aggcaaagcc cgtgagcagg
     2221 gcgaacacaa tgaccaggat ttggacgatc cacacgatcg cctgggtcac cgcatccatc
     2281 gctacgctcc cttgcgaacc tgcgccgcgc cgcccggcac gcccgccgga gccagcgctg
     2341 cgccctgcgg cgcgcggccg tcgacgtgcg ccgtcatccg gacctcacgg ccatcgaccg
     2401 acaccgccac cgcatccccg tcggagacgc ccagctccgc cgcatcgtcc gcgtgaagct
     2461 cgagcgtcgc attccggaga cgcggctgga acggcttcga ctggtcgatc agcgtgccgc
     2521 gatcgtacag ccggcgcgac gggacgacga ggaagccgtc gggctgcgca ggcgcgtcga
     2581 tccatatcaa gctcgcccgg ccgtccagtt cgacgccaga cttcgtctgc gcgccgagac
     2641 cgccgcgatt gtcataggcc gtgccgccaa agtacaagtc tttatcaccc accggcggcc
     2701 actgcggagc cgactgcgcc agtgctgcat acgataatcc ggcgtagcgc ggctgcgccg
     2761 atgcgatctc ggcgaaaacc tcttccgccg tgtgataatc ccaattgaga tcgagccgcc
     2821 aggcgatgtc ttgcgcgatt tcccagtccg ccttgccatc gcccagcgcg ggcagcgcgc
     2881 gatagaaccg ctgcacgcgg cggtcggcgc tggtagtcgt gccgtcgcgc tcggcgaacg
     2941 acaacgccgg cagcaccacg tcggcgtcct gcgccgtctg ggtcaagaac agctcctgca
     3001 cgatgcggaa cgggccatcg ttgccgcgtc gcctcggcgc atgtccctcg ccgaccggat
     3061 ccgtgccgac catatacagc acctccggca tcggctcggc gccgaagcgc agatcgccaa
     3121 tcagatcgtg cgtcccctgc gtgttgttgt gaggccacag cggcagcaga ccgttgttcg
     3181 gcctgccgaa atgcccggtg gcgacggcga tattcgcgca cgcctgcgcc agcgccgatg
     3241 cctgcggcag catctcgccg ccggtcttga cgagattgag agccatctgc ccgaagatga
     3301 tcacaacgtc agtcgcttcg gcgatgatct gggccgcgtc gcggatcgca ggtgccggca
     3361 cgcccgtcaa ctgctccacc ttttccggcg agtactccgc tgctctgctc ttcacttcgg
     3421 cgaaaccctc gacgccttcg ccccgatttt cactcaacaa ggcgttcaga ataccgaagg
     3481 cgacgtacga ctcggcgccg agcttgtagc ggagcgcgag ccgggcatag cgatccagct
     3541 tggtttcgta gccatgcgcg acgatcagtt gcgcgccgcg cttgaccgcg cccctgatgc
     3601 gcaggaacca gacgggcgct tcttcttcga cgtcgccggc aatcacgagg atcgcgctgc
     3661 cctcgcccat cttgccgaaa tcgctgccgg cgccgacgcc gtagcgcgtt gcaatctcgt
     3721 gggcggcgac gtgagggcgg gcgttgatgc gagccgactg caggccttcg ctgaacaact
     3781 tgcgaatcaa atacacgtcc tcgttcgcca ggcgatcgcc gatgagcgcg ccggcattcg
     3841 atccggcggc cttcagcctc gccgcaacga gcgccagcgc ttcgtcccac gacacgacga
     3901 acagccggcc gtccttgcgg atcaagggct tcaccaggcg ctcttcgctg agcgcgtagt
     3961 gatggccgaa gcggcccttg tcgcaaatcc agatctcgtt gacttcctcg ttctggcgcg
     4021 gcatcacgcg cagaatgtcc cagtgccccc tacgcgcgtt gctgcgccgc gtgccgagcg
     4081 tgatgttgca gccgatcgcg cagtgcgggc agatcgaggg tacgttagtc atctcccacg
     4141 gccgggcgcg gaagcggaag tcgctggtcg tcagcgcgcc gacgggacag atgtcggtcg
     4201 tattgcccga gaacttggag tagaaccctg gctcgctcaa tgtggcgatc tccagcgtcc
     4261 gcccacgctc gttgaagccg atcacgcgat cgtcggcgat ctcgtcctgg aagcggatgc
     4321 agcgcgcgca ctggatgcag cgttccttgt cgagaaagat cagatcgccc agcgggaagt
     4381 gcttgtcgag gcgctgtttg ccggcgaaat cgaagcggga cacgccggga ccgtagccca
     4441 ttgtcaggtt ctgcagcggg cattcgccgc ccttgtcgca gattgggcag tccagcgggt
     4501 gagaggtgag caggaattcc agcacgtccc gctgagccgc cctggccttg tcgctcttcg
     4561 tgcggacgcg catgccttcg ctcaccggcg tcgtgcagcc cgtctccagc tttatccccc
     4621 aggcgatctc cggccggccg tccggcccca cgacgacttg cttggtcgcc gggtcgagct
     4681 tggggcggcc gatctcgacc aggcacatgc ggcacatgcc caccggttcg agcttcgggt
     4741 ggtagcagaa gacggggatg tcggttccca tcttcttcgc cgcctccacc acgaccgtgc
     4801 cccgggggac ggagacgagt tgatcgtcaa tatagaggtt aaccagatcg gccattacgt
     4861 agtatccctg ccgttgaagt catacagcag tcactccggc tttgcacgtc gcgccacgcc
     4921 agcggccaga gccgtgccgc cagtcaacac gaatggcgca gatgccggtt gggcaaagaa
     4981 cctaatcaat cacgcgtcac gttttacccg tcacgtttta cgctgcctgc ttcacgggcg
     5041 caggtttggc gaccggcttg gccgcgggct tcgccgccgc cttcgcgcca tcgaccttcg
     5101 cctcgtagtc gcccctgaac agcttcaggc ttgacagcac cgggccggtg gcgaactcgc
     5161 cgagcgggca gaagcacttt ccgaccatct ggccggcaac cgtgcccaac tgttcgacgt
     5221 cggccttctg ccctttgccc gacgcgatct tctcgtaggt ttgcagcatc cagtgcgtcc
     5281 cctcgcggca cggcgtgcac ttgccgcacg actcgtgctt gaagaagcgc gtcactttgt
     5341 gcgccgccca ggcgacgtcg accgtgttgt ccataatgat gaccgaagcc gagccaagca
     5401 tcgagccggc agcggcgacg gattcgtagt cgagccgcac gtcgagcgcg tcctccgtca
     5461 gcagtgtcga cgacgcgccg gacggcatga tcgccttcac tgcccgatcc agctccggca
     5521 ggccctggcc atactctgcg ccgaagatca gatcgcggaa ggtgatcttg cccagctcga
     5581 a
//
LOCUS       DBNHXB010000378         2834 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1450446, whole genome shotgun sequence.
ACCESSION   DBNHXB010000378 DBNHXB010000000
VERSION     DBNHXB010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1450446"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="AC162G_15270"
     CDS             complement(<1..137)
                     /locus_tag="AC162G_15270"
                     /inference="COORDINATES: protein motif:HMM:NF013930.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HIR5077214.1"
                     /translation="MSVIDDIKDRIDIVDVVSETVKLRKSGRTHVGFCPFHSNTRTPS
                     FV"
     gene            complement(176..616)
                     /locus_tag="AC162G_15275"
     CDS             complement(176..616)
                     /locus_tag="AC162G_15275"
                     /inference="COORDINATES: protein motif:HMM:NF037832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HIR5077215.1"
                     /translation="MRSDSLLFIQVYTDEHVAPVLATLLRQRGYVAQSAQEAGMLGRD
                     DEEHLAYATQNNMAILTFNGKDFDRLAREWVAVGREHAGIVISPQLSRDRMGELLHSL
                     IRLLDSLTADEIRNQVVYLQRFGARRPRRISEIAAEYAASVTPL"
     gene            complement(606..968)
                     /locus_tag="AC162G_15280"
     CDS             complement(606..968)
                     /locus_tag="AC162G_15280"
                     /inference="COORDINATES: protein motif:HMM:NF016167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR5077216.1"
                     /translation="MAVQEAEVSTQERPTEHPHVVRIQKLSGEQAVIRGTRMPVWLIA
                     GFYKAGDTVDDILISYPHLSPAAVYDAVSYYHDHQAEIEAEIASQRIENILKESGAII
                     DERGFIRYPDLSHKNDAQ"
     gene            complement(1160..>2834)
                     /locus_tag="AC162G_15285"
     CDS             complement(1160..>2834)
                     /locus_tag="AC162G_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HIR5077217.1"
                     /translation="INQLLLPRFDDKAKKDGQFLAKGLNASPGAATGVAAFDADTAEA
                     WGKGGKAVILVRPETNPDDVHGMLVAKGILTQRGGATSHAAVVARGLGKPCVAGTEAI
                     RVDTEMKLFSANGRVIKEGDYISIDGTTGEVYAGQIATIAPDFGREKELAELLGWADE
                     IRRLGVWANADYPKDAANARMFGAEGIGLCRTEHMFFEQARLPFVQQMILNASEAQSK
                     LDALQRAEADLARRPDNREAKAAVAAAKKAVASSPAVKAYKGALAKLLPFQRKDFEGI
                     LKVMDGLPVIIRLIDPPLHEFLPSYEDLLVQATELRVKGKKGKKLQEVEKMLAAVASM
                     REMNPMLGLRGIRLGITYPDILKMQVRAIFEAACNMQAKGVKVKPEVMIPLTGHVNEL
                     AVSQGALEEVAKQVMREKGVEVEYKFGTMIEVPRAALTAGEIAEYAQFFSFGTNDLTQ
                     TTYGYSRDDAEGKFLLRYVDEKILPSNPFQELDREGVGKLIKMCVEAGRTTRPELEVG
                     ICGEHGGDPSSIEFCHEAGLNYVSCSPFRVPIARLAAAQAALGAVVRDK"
BASE COUNT          493 a          876 c          851 g          614 t
ORIGIN      
        1 acgaacgacg gcgtgcgcgt gttcgagtgg aacggacaaa agccgacgtg cgtgcggccg
       61 ctcttcctga gcttgaccgt ctcgctcacc acgtcgacga tgtcgatgcg gtctttgatg
      121 tcgtcgatga cgctcatgtt aaccaagggt gacagacaga tagcaagcgt agaatctaca
      181 aaggagtgac gctggctgcg tattcggcgg cgatttccga gatgcgccgt ggtcgacgcg
      241 cgccgaagcg ctgcaagtac acaacctgat tccggatttc gtcggcggtc aatgagtcca
      301 ggagacggat cagcgagtga agcagttctc ccattcgatc gcgactgagc tgaggtgaga
      361 tgacgatgcc tgcatgctct cgccccactg ctacccattc gcgggccagt cggtcgaagt
      421 ccttcccgtt gaaagtcaga atcgccatgt tgttctgcgt tgcataagcg agatgttctt
      481 cgtcgtcccg cccaagcatg cccgcctctt gcgcactttg cgcaacatag ccgcgttgtc
      541 gaagcagcgt ggccaagaca ggcgcgacat gctcgtctgt gtagacctgg ataaacaaca
      601 gagaatcact gcgcatcgtt cttgtggctt agatcaggat atctgatgaa tccacgttca
      661 tcgataatcg ctccgctttc cttcaggata ttctcgatgc gttgggaagc gatctctgct
      721 tcgatttctg cttgatggtc gtggtagtaa ctgactgcat cgtaaactgc cgcaggagaa
      781 agatgcggat agctaatcag aatgtcatcc acggtgtcgc ccgctttgta gaaaccggcg
      841 atgagccaga caggcatacg cgtgccccta atgacggctt gctcgcccga tagcttttga
      901 atgcgcacga cgtgcggatg ctcggtgggt cgttcttggg tggagacttc cgcttcttga
      961 accgccattg tgtcgtactc cgttgttcaa tcaggtgttg tgcctgccta ctgcctagtt
     1021 atcacagtgg caggaggtgc tgttggcatt gaaagtatac cctgaatcgc aaatggatgc
     1081 gactctggca gcggatgaaa aacggataaa cgggtgggcg ctcgcatccg tttatccgtt
     1141 cattcacacg atctgctgtc tatttgtcgc gcaccaccgc gccgagcgcc gcctgtgccg
     1201 ccgccaggcg cgcgatcggc acgcggaacg gcgaacagga tacgtagttc aggccggcct
     1261 cgtggcaaaa ctcgatcgaa gacggatccc cgccgtgctc gccgcagatg ccgacttcga
     1321 gttcgggccg cgtggtccgc ccggcttcga cgcacatctt gatcagcttg cccacgccct
     1381 cgcgatcgag ctcctggaac gggttgctcg gcaggatttt ctcgtcgacg tagcgcagca
     1441 ggaacttgcc ctcggcgtcg tcgcgcgagt acccataggt cgtctgcgtc aggtcgttcg
     1501 tgccgaacga gaagaactgc gcatattcag cgatctcgcc ggcggtgagt gcggcgcgcg
     1561 gcacttcgat catcgtgccg aacttgtact cgacctccac gcctttctcc cgcatgacct
     1621 gcttggcgac ctcctccagc gcgccctgcg acacggccag ctcgttgacg tggccggtga
     1681 gcgggatcat cacttcaggc ttgaccttga cgcccttcgc ctgcatgttg cacgccgcct
     1741 cgaagatggc gcgcacttgc atcttgagga tgtcagggta agtgatgccg agccggatgc
     1801 cgcgcaggcc cagcattggg ttcatctcgc gcatcgacgc tacggcggcg agcatcttct
     1861 ccacttcctg aagcttcttg cccttcttgc ccttcacgcg tagctcggtc gcctgcacca
     1921 gcaaatcttc gtacgaaggc aggaattcgt ggagcggcgg atcgatcagg cggatgatca
     1981 ccggcagccc gtccatcact ttgaggatgc cctcgaagtc tttgcgctgg aagggcagca
     2041 gcttggcgag cgcgcccttg taagctttca cggcgggcga cgaggcgacg gccttcttcg
     2101 cggcggcgac ggcggccttc gcttctctgt tgtcgggtcg ccgcgccaaa tcggcttcgg
     2161 cgcgctgcag cgcgtcgagc ttcgactgcg cctcgctcgc gttgaggatc atctgctgca
     2221 cgaacggcag gcgcgcctgc tcgaagaaca tgtgctcggt gcggcacagg ccgatgcctt
     2281 ccgcgccgaa catgcgcgcg ttcgccgcgt ccttcgggta atcggcattg gcccacacgc
     2341 ccagcctgcg aatctcgtcg gcccatccca agagttccgc gagttccttt tctctgccaa
     2401 agtctggcgc gatcgtcgcg atttgtcctg catacacctc gccggtcgtg ccgtcgatag
     2461 agatgtagtc gccctctttg atgacccggc cgttggcgct gaacagcttc atctccgtgt
     2521 cgacgcggat ggcctcggta cccgcgacgc atggcttgcc caggccgcgc gcgacgaccg
     2581 ccgcgtgcga ggtcgcgccg ccgcgttgcg tcaggatgcc tttggcaacc agcatgccat
     2641 gtacgtcgtc gggattggtt tcggggcgga ccaggatgac agccttgccc cccttgcccc
     2701 acgcttcggc cgtgtcggcg tcgaaagccg cgacgccggt cgccgcgccg ggcgacgcgt
     2761 tgagaccttt cgccaggaac tggccgtcct tcttcgcctt gtcgtcgaag cgcggcagca
     2821 gcaactggtt gatc
//
LOCUS       DBNHXB010000379         4855 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1282342, whole genome shotgun sequence.
ACCESSION   DBNHXB010000379 DBNHXB010000000
VERSION     DBNHXB010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1282342"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="AC162G_15290"
     CDS             <1..618
                     /locus_tag="AC162G_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077218.1"
                     /translation="QLQVLATDWENANATIRARPDWLLPAIYVALAAFVGTFANHALR
                     RVGAATATGVLTLFIRFGLIQLFNYHAITIDSWLPVLPPLIALDAWYAYRLRTGRSLS
                     SPLMNGSAATIGLLLVSIIVINRVFAHPQIGWDNIAPVMIACVLIGAGGSWIGQTLGN
                     YLASENKHLEAAEQPDARMRFVSPALFAGTLVFIVFFVVTATPPH"
     gene            648..1568
                     /locus_tag="AC162G_15295"
     CDS             648..1568
                     /locus_tag="AC162G_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077219.1"
                     /translation="MSDGSIDAQALSPALPDWLRTRTRPPWIAAPRWRALPEEDRQAV
                     LDEEHQAWVDWLNASPSRAGWPLEALAEATALFFGGLSQEGRAAEHPILPGDAGRGVF
                     GAWRTFGAQGAAAAQYLNLKKMFGSRFPNPHVNLCGQLSVISLLGLGVREGLQQFVNL
                     PTGDGDDILADPAQTTLGSKLVEFIQFTGGGRLRAEFLRAQNPAQPTPVFEPPDMLAQ
                     IDAGARLLALVNIDRNGRIKPMAESAKKIAHWVGVIDVVAPRKGATAIRVYNPFPNRE
                     EFYTWPNFRAAWKATQGNSSRFAMVIARPV"
     gene            complement(1597..2268)
                     /locus_tag="AC162G_15300"
     CDS             complement(1597..2268)
                     /locus_tag="AC162G_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5077220.1"
                     /translation="MSPDKVTIVLIDDHQVVREGLRAYLSTEPDIEVIGEASNGREGA
                     RLAAELVPDVALVDLVMPDPSPGPAGPMDGITTIREIKKLSPHTQVIVLTSYAEDEKI
                     FPAVRAGALSYLLKDVSAEDLVKAIHAAARGEATLHPQVAARLVSEVGGGDRRASGVD
                     TLTEREMEVLTLIARGMSNREIAQALVVTEKTVKSHVSNILGKLHLADRTQAAIYALR
                     QGIVP"
     gene            complement(2356..3276)
                     /locus_tag="AC162G_15305"
     CDS             complement(2356..3276)
                     /locus_tag="AC162G_15305"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5077221.1"
                     /translation="MAVTVVAILAVEVAMALGVIPRSPVFLLGAAAVAGGLFGFLTAR
                     AIKRRLRSIARAAAAIAAGDLSQRLAIDGDDEIAELAEQFNEMALRLDANAAELRRLA
                     AADERSRLAGELHDSVNQQIFSISMLAATARKKLGSHPASDDLARLETLARETHTEMR
                     ALVRELRPSALEHADLSTALKRYVADFGSRYGLTVVVDAPPIGRLPADLETALYRIAQ
                     EALGNAVRHASAKQVHLSLRRDDGAIELSVQDDGHGFDVQAHREGLGLHSMRERAQAF
                     GGTCEVRSEPGKGSSIYVRVRARGSSLRSE"
     gene            complement(3367..4116)
                     /gene="liaF"
                     /locus_tag="AC162G_15310"
     CDS             complement(3367..4116)
                     /gene="liaF"
                     /locus_tag="AC162G_15310"
                     /inference="COORDINATES: protein motif:HMM:NF021443.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HIR5077222.1"
                     /translation="MDTTARHPRFIVGVAFVLLGVLFLMSNVGLLYGINVWAVLWGLV
                     WLWLGAVVVGPRGRGVGAGRLALGLLLIAVGVITLARGAGLIGFSFGDLLFRYWPLIL
                     IALGVFLLYQYNRKPAPGLPSAVERIEHDSIFGDFKLTQPGWRLRDVRASSVIGDMKI
                     DLSKADIPDGETVLELSAVVGDIDVWAPLDLPVALDARCTFVSLNHFGRKQDVRLQRY
                     TDAPADFASSARRVRIQVDLVFGDLTLTRVG"
     gene            complement(4343..>4855)
                     /locus_tag="AC162G_15315"
     CDS             complement(4343..>4855)
                     /locus_tag="AC162G_15315"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011019818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIR5077223.1"
                     /translation="DLEAGPDAPDIIHVVIEVPKGSRNKYEYHKQAGTIFLDRVLYSS
                     LHYPGDYGLIPRTFYGDGDPLDALVMVGEPSFPGCVIVARPIGIFRMLDKGLPDDKVL
                     CVPATDPIFRDVYDLPDIPQHFLDEVAHFFSVYKDLEGVHVKKVGWERAEKARESIRH
                     AVALYKQQTW"
BASE COUNT          869 a         1584 c         1578 g          824 t
ORIGIN      
        1 caattgcagg tgctggcgac cgattgggaa aatgcgaatg cgacgattcg tgcacggccg
       61 gattggctgc tgcccgcgat ctacgtggcg ctggccgcct tcgtgggcac gttcgccaat
      121 cacgccttgc gccgtgtggg ggctgccacc gcgaccggtg tcttgacgct gttcatccgc
      181 ttcggcctga tccagctctt caactatcac gccatcacga ttgactcgtg gctgccggtg
      241 ctgccaccgc tgatcgcgct cgacgcgtgg tacgcctatc gtctcaggac aggtcgatcc
      301 ctatcgtcgc ccctcatgaa cgggagtgcc gcgacgatcg gcttgctgct ggtctcgatt
      361 atcgtcatca accgcgtgtt cgcgcacccg cagatcggat gggacaacat cgctccggtc
      421 atgatcgctt gcgtgctgat tggcgcgggc ggcagttgga tcggccaaac actgggtaac
      481 tatctggcga gcgaaaacaa acacctggaa gcggcggagc agcccgacgc gcgaatgcgt
      541 ttcgtgtcgc ccgcgctttt tgccggcacg ctggtgttca tcgtgttctt cgtggtaacg
      601 gccacgccgc cgcattagca ggtcgctcaa cttcaatgga ggccaacatg tctgacggct
      661 caatcgacgc acaggcgctc agcccggctt tgcccgattg gcttcgcacg cgcacgcggc
      721 cgccgtggat cgccgcgccg agatggaggg cgctgccgga ggaggaccgg caggcggttc
      781 tcgacgaaga gcatcaggcg tgggtggact ggctcaacgc gtcgccatcg cgcgccgggt
      841 ggcctcttga ggcgctggcc gaggcgacgg cgcttttctt cgggggcctc tcgcaggaag
      901 ggcgcgcagc cgagcacccg attctgcccg gcgacgccgg gcgcggcgtg ttcggcgcgt
      961 ggcggacatt cggcgcgcag ggcgcggcgg cggcgcagta tctcaacttg aagaagatgt
     1021 tcggcagccg ctttccgaat ccgcacgtca acctgtgcgg acaactgagc gtgatcagcc
     1081 tgctagggtt gggcgtccgg gagggtcttc agcagttcgt gaacctcccg accggcgacg
     1141 gcgacgacat cctcgccgac ccggcccaga cgacgctcgg gtcgaagctc gtcgagttca
     1201 tccagtttac tggcgggggg cggctgcgag ccgaattcct gcgtgcgcag aacccggccc
     1261 agcccacgcc ggtattcgag ccgccggata tgctggcgca gatcgacgcg ggagccaggc
     1321 tgctggcgct ggtcaacatc gatcggaacg gccggatcaa gccgatggcc gagagcgcca
     1381 agaagatcgc ccactgggtc ggcgtgatcg acgtggtcgc tccgcgcaag ggcgcgacgg
     1441 cgatccgcgt gtacaacccg ttcccgaacc gcgaagagtt ctacacctgg ccgaatttcc
     1501 gggccgcgtg gaaagcgacg cagggcaact cgtcgaggtt cgcgatggtg atcgcccggc
     1561 ccgtgtgatg gtgttgacgc atccccatgc gtcaacttaa ggaacaatgc cttgacgcag
     1621 cgcgtagatc gcggcctgtg tccgatcagc caggtgtaac ttgcccagga tattgctgac
     1681 gtgcgatttc accgtctttt cggtgacgac gagcgcctgg gcgatttcgc ggttgctcat
     1741 gccgcgcgca atgagcgtca agacttccat ctcgcgctcg gtcagtgtgt cgacgcccga
     1801 cgcgcgcctg tcgccgccgc cgacttcgct caccagccgg gccgccacct gcggatgcag
     1861 tgtggcctcg ccgcgtgctg cggcatgaat cgccttgacc aggtcctcgg cgctcacgtc
     1921 cttcaggaga tagctcagcg cgccggcgcg caccgccgga aagatcttct cgtcttcggc
     1981 gtaagaagtc aggacgatga cctgcgtgtg cgggctgagc ttcttaatct cgcggatggt
     2041 cgtgatgcca tccatcggtc cggccgggcc gggcgacgga tcgggcatca cgagatcgac
     2101 gagcgccacg tcaggcacca gctcggcggc aagcctggct ccctcgcggc cgttgctcgc
     2161 ctcgccgatc acctcgatat ccggctcggt cgaaagatag gctctcagcc cttcacgcac
     2221 gacttgatga tcgtcgatca agacgatcgt gactttatca ggcgacatga acgcctcctt
     2281 ggcaacggcc gcgcggacgc gggctacgag catctcaggg ccacgagcct ctcagcgcct
     2341 cagcgcccca ccgtgtcatt ccgaacggag tgaggaacct ctcgcccgca cgcgcacgta
     2401 gatgctgctg ccctttcccg gctcgctgcg cacttcacag gttccaccga acgcctgggc
     2461 gcgctcgcgc atgctgtgca gccccaggcc ctcacgatgt gcctgcacgt cgaagccgtg
     2521 cccgtcgtcc tggacgctca actcgatcgc gccgtcgtcg cggcgcaggc tgagatgaac
     2581 ctgcttcgcc gacgcatgcc gtacggcgtt gcccagggct tcctgcgcga tacgatacag
     2641 tgccgtttcc agatcggccg gcaggcggcc gatcggcggc gcgtcgacga cgacggtcag
     2701 gccataccgg ctgccgaaat ccgcaacgta tcgtttcaag gcggtggata aatccgcatg
     2761 ctcgagcgcg gaaggacgaa gctcgcggac cagcgcccgc atttcggtgt gggtttcgcg
     2821 cgccaacgtt tcgaggcggg ccagatcatc ggacgcggga tgactgccca gtttcttgcg
     2881 cgccgtggcg gcgagcatgc tgatgctgaa aatctgctgg ttcaccgagt cgtgcagttc
     2941 gccggccagg cggctccgct catcagccgc cgccaggcgg cgcagttcgg cggcgttggc
     3001 gtccagccgc aaggccatct cattgaattg ctcggccagc tcggcgatct cgtcatcgcc
     3061 gtcgatagcc agtcgctggc tcaggtcgcc ggccgcgatt gcagccgccg cgcgcgcgat
     3121 cgagcgcagc cgtcgcttga tggcgcgcgc cgtcaggaag ccgaacaggc cgccggcaac
     3181 ggccgccgcc cccaagagga atacggggct gcgcgggatc acacccagcg ccatagccac
     3241 ttcgacggcc aggatggcga cgacggtcac cgccacagtg gtcgccgtca ggcgccactg
     3301 aatgccagag tatagcggcc gcgaacggaa gggagattgc cggttcacga gttgactgtg
     3361 gggacgctag ccgacgcgcg tcaaggtgag atcgccgaag accagatcga cctggatgcg
     3421 cacgcgccgc gccgacgacg cgaaatcggc gggcgcgtcg gtgtaacgct gcagcctcac
     3481 gtcttgctta cggccaaaat ggttgagcga gacaaaggtg cagcgcgcgt ccagcgcgac
     3541 cggcaggtcg agcggcgccc acacgtcgat gtcgccgacg acggcgctca gctcgagcac
     3601 ggtttctccg tccggaatgt ccgccttgga cagatcgatc ttcatgtcgc cgatgacgga
     3661 gctggcgcgc acgtcgcgca gacgccaacc gggctgtgtc agcttgaagt cgccgaagat
     3721 ggaatcgtgc tcgatgcgct caaccgcgga aggtaagccg ggcgccggct tgcgattata
     3781 ctggtacagc agaaacactc ccagcgcgat caggatcagc ggccagtacc ggaagagcaa
     3841 atccccgaac gagaagccga tcagcccggc gccgcgggcc agcgtgatca caccgacggc
     3901 aatcagcagg aggcccagcg ccagcctccc cgccccgacc ccgcggccgc gtggcccgac
     3961 gaccaccgcg ccgagccaca gccagaccag gccccacagg acagcccaga cgttgatgcc
     4021 atacaagagg ccgacgttgc tcatgagaaa cagcacgccc agcagcacga atgcaacgcc
     4081 gacgatgaaa cggggatgcc tggcagtggt atccatgaga gtgcccctaa acgcaagatc
     4141 gccctgagtg tagcgcaggg gagcgccgcc gtcaccgggc cagcggctga aatcgacctc
     4201 cgacttgagg cggaaagccg cactgctgag tctcagtaga tccaaattcg gaagcagagc
     4261 gggctcccac gcccgcaaat cggcggcatg ggagccgcct ccgctaacaa tattggatct
     4321 gcggagtctg gcggcggcgc tgtcaccagg tctgctgttt gtacagcgcg acggcatgcc
     4381 ggatgctctc acgcgctttt tcggcgcgct cccagccgac cttcttgaca tgcacacctt
     4441 ccaggtcctt atagacggag aagaaatgag cgacttcgtc caggaagtgc tgtgggatgt
     4501 cgggcaggtc gtagacatcg cgaaagatgg gatcggtggc cggcacgcac agaactttgt
     4561 cgtcgggcaa gcccttgtcc agcatccgga aaatcccgat cgggcgggcg acgatgacgc
     4621 agcccggaaa actcggctcg ccgaccatga ccagcgcgtc gagcgggtcg ccgtctccat
     4681 agaacgtgcg cgggatcagg ccgtaatcgc cgggatagtg aagcgacgaa tacagcacgc
     4741 ggtcgaggaa gatcgtgccc gcctgcttat gatactcgta tttgttccgc gagcctttcg
     4801 gcacttcgat gacgacgtga atgatgtcgg gcgcgtccgg cccggcctcc agatc
//
LOCUS       DBNHXB010000380         6201 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3295362, whole genome shotgun sequence.
ACCESSION   DBNHXB010000380 DBNHXB010000000
VERSION     DBNHXB010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3295362"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1641)
                     /locus_tag="AC162G_15320"
     CDS             complement(<1..1641)
                     /locus_tag="AC162G_15320"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5077224.1"
                     /translation="MHHKRVTTTASLLVLVSLLLAACATPAATPETVVVTAPPEVQVV
                     TATPPPVPPAEPKTLVICQGQEPDSLYTYGTDMLAAAHIQEAIWDGGDWQPAGMDSRT
                     YDFQPVILEKLPSVDDGDAVLNAVTVGEGDRVLNDAGDPVELAAGETIRPAGCLTSDC
                     AVTYEGGDVEMDQFVVTFKMLPGLTWSDGEPLTADDSVYGFELLADPDTPESKFTTDR
                     TTSYEATDDVTAVWTGIPGYIDATYFTNFFVPLPRHLWQEQLGYSAADLVTAEESSRT
                     PLGWGPFVITEWVPGDHITLEKNSNYFRASEGLPKVDTIVVRFTGEDPNPVIAAALAG
                     ECDIIDQTSGLESQSKLLIELEASGQLIPTFVTGTQWEHVDFGINVVDSYERPDFFED
                     VRFRQAFAQCLGRQEVVDAVLFGRSVVIHDFIPPEHPMYAEDVTQWPDDPAAGMALLD
                     EIGWVDDDGDPATPRVAQGVEGIPDGTLLSFKWQSTTAELRRTYMPIYQENLRECGIE
                     VTLENLSAAEYFADGPDGPLFGRAYDVGSFAWLTGVVPG"
     gene            complement(2205..4892)
                     /locus_tag="AC162G_15325"
     CDS             complement(2205..4892)
                     /locus_tag="AC162G_15325"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5077225.1"
                     /translation="MSLTPALLTTKLAIPPTRPDLVTRPRLIERLHQARHYKLTLVSA
                     AAGFGKTTLLSMWSQLTPLPVAWVSLDADDNDPARFLAYLIAALRTIHTSIGDAAQEL
                     LHSPQLLLQAVVATLINEIAAGRDDFVLVLDDYQSCDDPGIHWTVAFLLDHMPAHVHL
                     VIASRSDPPLPLPRLRARREMLELQTDDLRFTPEETTAFLTRVMGLELSAQDIAALDA
                     HTEGWIAALQLAVLSMQGLSDTAGFIATFTGSHRHIMDYLVEEVVSRQPPDVQSFLLR
                     TAVLDRLCGSLCDAVTGRDDGPAILDQLERANLFLVRLDEGQHWYRYHHLFADALCAR
                     LHQTQPSVVGELHRRAAEWHERNGSVETAIGHALAGQDFETAARLIECIVQVKCARGE
                     LATSARWLNALPDEFVRARPLLAMYQAQTLLYDGHPDRAEQLLQNAERILQDEAPADH
                     REHMSGRLTALRAVIACVQGRITESLDLARQALERVPAHDAATRGRAVNIMGIALSLQ
                     GDVAESGRAFAQALHLGQTTGHIYLTLTAFSNLADAYHMQGRLRQAVETCDRALGFLS
                     ERSWQRIPAASAVYLKLADLDYEWNKLDAAERHLTTCLELAEQGGIADVLLNATLTRA
                     SLKQAQGDRDDAIAAIQRANELAQRYNPSFTLMAPRLARIWLSLGDIDTAAGTAQTAL
                     QQPGSETPDYSREFVDLALARVHLAQDAPDAALQRLERWLEPAESAGRAGSVIEIHLL
                     KALALETQGDTPQALAALHAALSLAEPEGYTRLFLDEGAPMDALLSKATECGAASGYA
                     SRLLAAFASHRRPATHAHTLGDTLPVPGSYTLIEPLNDRELQVLSLIADGLSNKEIAR
                     EKAYTLSTVKWYVRNIYEKLQVHSRTQAIARARAVGLLG"
     gene            5470..>6201
                     /locus_tag="AC162G_15330"
     CDS             5470..>6201
                     /locus_tag="AC162G_15330"
                     /inference="COORDINATES: protein motif:HMM:NF012361.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR5077226.1"
                     /translation="MGLSKQYDPNSAEPRIQAGWQASGVYHFSRDAAGPVYSIDTPPP
                     TVSGHLHLGHVYSYSHADFLARFWRMNGYNVFYPMGYDDNGLPTEHLVESRLGTTAQQ
                     AGRRAFVEKCLEVGEKAEQEYQELWQRLGLSVDWRHTYRTIDDLSRRTSQWSFIDLYR
                     KGLAYRQEAPAIWCPECRTAIAQADLNDLDRDSEFVTLAFRFEHGETLPIATTRPELL
                     PACVAAFVHPDDERYKRLVGRQLTVP"
BASE COUNT         1104 a         1952 c         2064 g         1081 t
ORIGIN      
        1 ccccggcacg acacctgtca gccaggcaaa cgagccgacg tcgtaggcgc gcccgaacag
       61 cgggccatcc gggccatccg cgaagtactc agcggccgac aggttctcca gcgtcacctc
      121 gatgccgcac tcgcgcaggt tctcctggta gatcggcatg taagtcctgc gcagctcggc
      181 cgtcgtgctc tgccacttga atgacagcag cgtgccatcg gggatgcctt ccacgccctg
      241 cgcgacgcgc ggcgtggccg gatcgccgtc gtcgtccacc cagccgatct cgtccagcag
      301 cgccatgccc gccgccgggt catccggcca ctgcgtgacg tcctcggcgt acatggggtg
      361 ctcgggcggg atgaagtcgt ggatcacgac cgagcggccg aacagcacgg catcgacgac
      421 ttcctggcgg cccaggcact gtgcgaatgc ctggcggaag cgaacgtcct cgaagaagtc
      481 ggggcgctcg tacgagtcga cgacgttgat gccgaagtcg acgtgttccc attgcgtgcc
      541 ggtcacgaac gtcgggatca actggccgct cgcctcaagc tcgatcagca gtttggactg
      601 gctttccaga ccgctcgtct ggtcgatgat gtcgcactcg ccggccagcg cggcggcgat
      661 caccgggttc ggatcctcgc cggtgaagcg gacgacaatc gtgtcgacct tgggcaggcc
      721 ttccgaggcg cggaagtagt tggaattctt ctcgagggtg atgtgatcgc cggggaccca
      781 ctccgtgatg acgaacggcc cccagcccag cggtgtgcgg gacgactctt cggcggtgac
      841 caggtccgca gcactatagc ccaactgctc ctgccacagg tggcgcggta gcggcacgaa
      901 gaaattggtg aagtaggtgg cgtcgatgta gccggggatg ccggtccaca ccgccgtcac
      961 gtcgtccgtc gcctcgtagg aggtggtgcg gtcggtcgtg aacttgctct ccggcgtgtc
     1021 gggatcggcc agcagctcga agccgtatac cgagtcgtcg gcggtcagcg gctcgccgtc
     1081 cgaccaggtg aggccaggca gcatcttgaa agtgacgacg aactggtcca tctcgacgtc
     1141 gccgccctcg taagtcacag cgcagtcgct ggtcaggcag cccgccgggc ggatcgtctc
     1201 gccggccgcc aattcaactg gatcgccggc gtcgttgagc accctgtcgc cttcgccgac
     1261 cgtgacggcg ttaagcacgg cgtcgccgtc gtcgacgctg ggcagcttct ccaggatgac
     1321 cggctggaag tcgtaggtgc ggctatccat accagcaggt tgccagtcac caccgtccca
     1381 gatggcctcc tggatgtgag cggcggccag catgtcggtg ccataggtgt acaacgaatc
     1441 cggctcctga ccctggcaga tgaccagcgt cttcggctca gccggtggca ccggcggcgg
     1501 cgtggctgtg acaacctgta cctccggcgg agccgtcacg acgacggtct ccggcgtcgc
     1561 cgctggagtg gcgcaggccg ccagcagcag ggataccagc acgagcaggc tggctgtcgt
     1621 cgtcacccgt ttgtggtgca tgtcttctct ctcctttgta gattggctta gagagtgttt
     1681 tgagaatcag cattttcaga cactctctta caacgaaggg cggaaacgaa aacggcctga
     1741 gtgtctctcg atagcatcac ctcctttcgc gcttccgtac ccgtggtcaa atgaaggatt
     1801 agaaggggtg cgccctggta gcacaggatt aggaggggag ccgctggtcc cctccctggt
     1861 ggagaagaag actttgcatc accaaagcct cttcagtggg gagggtaaca tgacagggcg
     1921 tatccgtcac cggtcatcgg gacggtgcgc tgccgataaa aagatagtgg atgcgacgtg
     1981 aggagagggg gtggtgcgac gcgagaagag aacgacagcg ggcgccagca ggctgtcgga
     2041 gaacccagca gaggcggctc cttgcgaaac accccgtagc cgaagccctg accctgagtt
     2101 ttgcgaaggg gaaggggcag cggagtgggc acaccgccga ttcagcgcgt ttcaaggcaa
     2161 attgccgggc atgggagccg gcccgctctc tctccggcga gctgctagcc cagcaggccg
     2221 accgctctgg cgcgcgcgat cgcctgagtg cggctatgca cctgcaactt ttcgtagatg
     2281 ttgcggacgt accacttgac ggtactcagg gtgtaggctt tctctctcgc gatctctttg
     2341 ttggagaggc cgtcggcgat aaggctcagc acctgcagct cgcgatcgtt gagcggctcg
     2401 atgagggtat aggagccagg cacgggcagc gtgtccccga gcgtgtgcgc atgtgtggca
     2461 ggccggcggt gtgaggcaaa tgcggccagc aatctgctcg cgtaacccga cgccgcacca
     2521 cactcggtcg ccttgctcaa gagcgcatcc atcggcgccc cttcatccaa gaagaggcgc
     2581 gtgtagcctt ccggctcggc gagcgagagc gccgcgtgca gcgccgccag cgcttgaggc
     2641 gtgtcgcctt gcgtttcgag cgcgagcgcc ttcagcaaat gaatttcgat aacgctgccg
     2701 gcgcggccgg cggattccgc cggctccagc cagcgttcga gccgctggag cgccgcatcg
     2761 ggcgcgtcct gggccaggtg cacgcgcgcc agtgccaggt ccacaaactc gcgcgagtaa
     2821 tcgggcgttt cgctgcccgg ctgttgcagc gccgtctgcg cagtgcctgc cgccgtgtcg
     2881 atgtcaccca ggctcaacca gatacgcgcc aggcgcgggg ccatcagtgt gaaggacggg
     2941 ttgtaccttt gcgccagctc attggcacgc tggatcgcag caatggcatc gtcgcgatcg
     3001 ccctgcgcct gcttcaggct agcccgcgtc agggtggcgt tcaacaggac gtcggcgatg
     3061 ccgccttgct cggcgagctc gaggcaggtt gtcaggtggc gctctgcggc gtccagcttg
     3121 ttccactcgt agtcgagatc agccagcttg aggtaaaccg cgctcgcggc tgggatacgc
     3181 tgccagctac gctccgagag aaagcccagc gcccggtcgc aggtttcgac ggcctggcgc
     3241 agccgacctt gcatgtggta cgcgtcggcg aggttgctga aggcggtcag tgtcagatag
     3301 atgtgccccg tggtctgccc caggtgaagc gcctgcgcga aggcccgacc cgattccgcc
     3361 acgtcacctt gcagactgag cgcgatgccc atgatgttga ccgctcggcc tcgcgtcgcg
     3421 gcgtcgtggg ccggcacgcg ttcgagcgcc tgtcgggcca ggtcgagtga ctcggtgata
     3481 cggccttgca cacaggcgat gacggcgcgc agcgcggtga ggcgcccgct catgtgctcc
     3541 cggtgatcgg caggcgcttc atcctgaagg atacgctcgg cattctgtag gagttgctcg
     3601 gcccggtctg gatgaccgtc atacaggagc gtctgcgcct gatacatggc gagtaggggc
     3661 cgagcgcgca caaactcgtc gggcaaggcg ttcagccagc gcgcggaggt cgccagctcg
     3721 ccgcgcgcgc acttgacctg cacgatgcac tcgatgaggc gcgcggcggt ctcgaaatct
     3781 tgtccggcca gcgcgtgacc gatcgcggtt tcgacggacc cgtttcgctc gtgccactcg
     3841 gcggcgcgac gatgcagctc cccgaccacg ctcggttgtg tctggtgcag gcgagcgcac
     3901 agggcgtcgg cgaacaggtg gtgatagcga taccagtgct gcccttcgtc cagccgcacg
     3961 aggaacagat tggcgcgttc caactgatcc aggatggccg ggccgtcgtc ccgacccgtg
     4021 acggcgtcgc acagcgatcc gcacaatcga tcgaggacgg cagtgcgcag caggaacgac
     4081 tgcacgtcag gcggctgacg gctcaccact tcctcgacca ggtagtccat gatgtggcga
     4141 tgactgccgg tgaaggtggc gataaacccc gcggtgtcgc tcaggccttg catggagagc
     4201 acggccagtt gcagggcggc gatccatccc tcggtgtgcg cgtcgagcgc ggcgatgtcc
     4261 tgggctgaaa gctccagacc catcacccgc gtgagaaacg ccgtggtttc ctccggcgtg
     4321 aagcgcagat cgtccgtctg cagctcgagc atctcgcgcc gggcgcgcag gcgcggcagc
     4381 ggcaggggcg gatcgctgcg actggcgatg accaggtgca cgtgcgccgg catgtgatcg
     4441 agcagaaaag cgaccgtcca gtgtatgccg ggatcatcgc acgactgata gtcgtccaac
     4501 acgagcacga agtcatcgcg cccggcggcg atttcgttga tcagcgtcgc cacgaccgcc
     4561 tggaggagta gttgcggaga gtgcagcagc tcctgcgctg catccccaat gctcgtatgg
     4621 atggttcgca acgcggcgat gaggtaggcc aggaaccgtg ccggatcgtt atcgtccgcg
     4681 tcgagcgata cccaggccac gggcagcggc gtcagttggc tccacatgct caacagcgtc
     4741 gtcttgccga agccggcagc cgccgacacg agcgtcagct tataatgccg ggcctggtgc
     4801 agccgctcga tcagccgggg acgcgtgacc agatcggggc gggtgggcgg gatcgcgagt
     4861 ttcgtcgtga gcaacgcggg cgtgagagac atcttccaca gccgatacgc ttgaactggt
     4921 gattgcacac atctcgctcc gcgcgccgtc ctcggcgcgg gcagtagccg ccggggacgg
     4981 cggttagagc acttgtgggt aatcaccggg cttgaaccgt tgaactttgc agtattactc
     5041 gcaaccgggt caaacagttc atttaccaaa actgaaccta ttgacacctc ctcaatgtgg
     5101 cgtataatga ccaataacca atatccggta cgctggaaac tgataaccga caactgatca
     5161 ctgaccactg acaacaacat acctgcatcc gggaggagta ggcggccggt gcgctgccag
     5221 agaaggaaag gccacaggct gagagcctcc ccagcaagcc gctgccgaac gtcgcccgga
     5281 ctgactgccg aagaaattcg cgcgtgcgga aagtagaccg gcacgggtaa gcccgttaca
     5341 gcgaggcccc gatgctggtc ggggcggcga gtgcgctgca caatcatgtg cggcgaagtg
     5401 gggtggtacc acggaaggcg tgcccttccg tcctcatgga cggaggggca ttttgttttg
     5461 gagggcaaca tgggactgtc gaagcaatac gatccgaaca gcgcagaacc gaggattcag
     5521 gccgggtggc aggcgtcggg cgtctaccac ttctcacgcg acgccgccgg gccggtctac
     5581 tcaatcgata cgccgccgcc gacggtctcc ggccatctcc acctgggaca cgtctactcg
     5641 tacagccacg ccgacttcct ggcgcgcttc tggcgcatga acgggtataa cgtcttttat
     5701 ccgatgggct acgacgacaa cgggctgccg accgagcacc tggtcgagag ccggctcggc
     5761 accaccgcgc agcaggccgg ccgccgcgca ttcgtcgaga agtgcctcga agtgggcgag
     5821 aaggccgagc aggagtacca ggaactctgg cagcgcctgg ggctgtcggt cgattggcgg
     5881 cacacgtacc gcaccatcga cgatctctcg cggcgcacgt cgcagtggtc tttcatcgac
     5941 ctgtaccgca agggactggc ctaccggcag gaagcgcccg cgatctggtg ccccgaatgc
     6001 cgcacggcca tcgcgcaggc ggatctgaac gatctggatc gcgatagcga gttcgtcacg
     6061 ctcgcgtttc gcttcgaaca cggcgagacg ctgccgatcg cgacgacgcg gcccgagctg
     6121 ttgccggcgt gcgtggccgc cttcgtccat cccgatgacg agcgctacaa gcgcctggtg
     6181 ggtcgacaac tcaccgtgcc c
//
LOCUS       DBNHXB010000381        24049 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2122198, whole genome shotgun sequence.
ACCESSION   DBNHXB010000381 DBNHXB010000000
VERSION     DBNHXB010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 24049)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24049)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24049
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2122198"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="AC162G_15335"
     CDS             complement(<1..225)
                     /locus_tag="AC162G_15335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5077227.1"
                     /translation="MNDPSVLIVEDDQDVAELYSRVLESSGLTTEIIRTGEAALERLA
                     ATAPDLVVLDLKLLPHVSGTDVLLRIRADPR"
     gene            complement(949..1830)
                     /locus_tag="AC162G_15340"
     CDS             complement(949..1830)
                     /locus_tag="AC162G_15340"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR5077228.1"
                     /translation="MDLIELIEQALARMGQDFPVLIQSIVIGLIVFAIGYVLTRVMQR
                     IIVRAIHNTPAGLTVERAMSRVVGIIGVTLSALTALATMGLDVAAVIAALGLGSLAIG
                     LALKDTIENAITGVLLLIQRPFKVGDIIKVGDVMGVVSDVAIRTTNVKTFDGLHVLIP
                     NRQVYNEVITNWSYYPTRRITATVGVAYDTDLPKAYRVLKEAVVSTPGVLAEPEPLIS
                     FEAFDDSSIRMVFRFWIDWQATNPVDAQTDVTQSIIDAARREGIDIPFPIRTVYVQHP
                     AQVSAAENTTTRSAGSA"
     gene            complement(1832..2305)
                     /locus_tag="AC162G_15345"
     CDS             complement(1832..2305)
                     /locus_tag="AC162G_15345"
                     /inference="COORDINATES: protein motif:HMM:NF017774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR5077229.1"
                     /translation="MIQLLIDGHNLIPRIPGLSLSQIDDEEKLVGLLRKYAAQHRAHV
                     VVVFDSGNLAGRSRELSGGGVEAIFAGSGTIADRILIERIRELKRPQQWKLVSSDRAI
                     QQEARRRRLQVVESAHFAGQLAPTRGSAEQVREDPDEKPGVEGDIDEWLDLFKRR"
     gene            complement(2426..5734)
                     /locus_tag="AC162G_15350"
     CDS             complement(2426..5734)
                     /locus_tag="AC162G_15350"
                     /inference="COORDINATES: protein motif:HMM:NF024754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR5077230.1"
                     /translation="MNWLRGLNPQQRMAVEAGDGPALILAGPGSGKTRVLTHRVAYLI
                     EARGVDPRHIMAVTFTNKAAREMKDRLDRLLGPNRTAELTLGTFHANCARFLRRDAPH
                     LGISRDFVIYDADDQEALVKRALKDLNVDDKKYKPSSVLNAISAAKSELIPPGAYVAR
                     TYWHEAVGRVYAHYQDLLRANNALDFDDLLMAVVQLFDANPVVLGYYQNRYRYILVDE
                     FQDTNTAQYTLLRQMAGRSRNLFVVGDEDQCLTAGAAIQTPSGRKPIETIRIGEKVLA
                     ASGRGATLPTEVVYVGSRPYRGELIRVTTRQGYSFRATPNHIVFARLSASAGIHYVYL
                     MQKEGVGYRIGVTSGARSDGVRPEWQSGLRVRGNQEQADRVWILRACSSRDEALYWEA
                     FLAFQYGIPTTVFHVAGRRLRMSQVTVDRLYQDIDTGERAERLLADLMMDTRYPHYIP
                     KAKELRHVVNLRYFSDSRRSERTPWNAHRVSVNCEDPALRRKLEARGYSTRRGGGNTW
                     RAEFSRLNFDDAQALAGEISRVCGNLEISVGAFLVESSSDEIARRFSLMPVSHLHPTM
                     IVAAERQGRIVQDEIVQIDREAYKGKVYDLEVQHLHNYLAGGVVVHNSIYRFRGADFR
                     NVRRFQQDYPDAQVILLEQNYRSTQTILDAARGLIRRNRQRTDKRLFTERSGGAPLSI
                     YEAYNEDDEALYVVNTIEDLIRSRQYKAADFAVFYRTNAQSRPLEEAFIRAGVPYRLV
                     GGTRFYARREIKDVLAHLHLVHNPHDAVSLARIVNVPPRGVGAKTLAALDGAARATRQ
                     SPYTLIAELRAGRGNPALVSSLTGKARQALVDFATLLDRWIAKKDRYGSVADLLDAVV
                     KDSGYRAYVDDGSEEGRERWENVRELRKVASDYTHAVGGDPLAEFLEDVALVSDVDSL
                     DDTQRVDAPTLMTLHSAKGLEFPVVFITGLEEGVLPHARSLEDPDEMEEERRLAYVGI
                     TRAQDRVYLSYAFRRTAYGSSEVNAPSRFLSDIPTALLSGSMSFAGATPRAAAAQRAA
                     TWASAQRSSPPARPSREPAFRPGQRVKHARFGEGVVVESRVQGDDEELAVAFEKAGIK
                     RLLASFANLEKLPG"
     gene            complement(5875..7662)
                     /locus_tag="AC162G_15355"
     CDS             complement(5875..7662)
                     /locus_tag="AC162G_15355"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HIR5077231.1"
                     /translation="MTTRPSVRCLVALSLAILLLSVFASILGQTSAIQAKAADSERYR
                     SVSGNDYTVGGPCGATIQACIDNPIVVAGDRILIPAGLYTESLVLNKAVSLIGADAGT
                     TIVHAVIDDRAMLITGSTVTATTIISGLTFAGGNLTGFPCPAGCGGGVLITGMARPLI
                     QNSTIVDNRSDFHGGGLYIDASGLMTLTNVTVARNVSDFGNGGGLYALSSVALIGGRF
                     EQNDGSAGGGVYVSNTLALSGTVFVSNTAFGRGGGAYVIGTATVTAGEFENNHSTSSN
                     PTTSLGGGLSVSGTLTLRGTQFISNSAQHGGGASVLGSTSLTGATFERNAAKYVGGGL
                     YTRGTVVLRDTDFRSNTAEFGGGGMRADGPITLSGGIFQNNRVGLTFGLGFGGGMDAF
                     DALTISGTQFISNATPGSGGGVFHSSVFEGPIGSGRIVNALFARNTAGAGGAALRLGS
                     GGDVVILHTTVADGSLNPKQAIVVVSGTVGITNSIIASHTAGISQTDSTVYADYNLFY
                     GNTIGKTGTIGGGAHDQTGDPRFADPAGDDYHLSAGSAAIDKGIGVGVTTDIDGDTRP
                     QGSGFDIGADEYYPPFGYRINLPIVLKNF"
     gene            complement(7686..9923)
                     /locus_tag="AC162G_15360"
     CDS             complement(7686..9923)
                     /locus_tag="AC162G_15360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR5077232.1"
                     /translation="MKRSRYVLYAGIALLTAVALELWQRSKRRPITPDTRSGALAPPA
                     APVEKLTPAVVSLPLPAGMQRRLTLPLLGAALVLALLGQAVFTEGPDSPNLSTGVALF
                     SIAIVLFTVAAAWAGVAVETAETWRASERHSQVEPTREPKVDESQPVFTSLRRQPRRI
                     ALLGAVALLTIILLDLLRADPSLPSYTLPFLLWLSAIGLYIRAVVPWPLPTRPHTGSW
                     LKAHWPHFLILAAIVWAAFALRVWNIEGIPPTLGGDEGTFGLESIRTFTGEIRNPFTT
                     GWLSVPTMSFYFNSLTIRMLGQTVFALRLPWALVGTLTVVIVFQLVRRLTGAALALMA
                     AALLATYHFHIHFSRLGVNNAADPLLIALVLYLLYRAYDERSVLEWALCGVAVGVAQY
                     FYFGARFAPVLVGAMIVYFVARDGRHFLRVHGGGIVIMAGAAIVAGAPMIQFAIRYPQ
                     DYNARVNQISIFQTGWLQRAQELTGQDEVSILLDQLRRAALAFNVYPDRTSWYGSPRP
                     LFDNATGVLFLLGLGYATLRLGDRRLFPMVAWWWGAMILGGALTQGPPSSQRLVTLGP
                     PAVFFVALALLKIGQILRSVWDAWTPHIFALYLIPSMLVIIIASVQWYFVEFTPLRLY
                     GDLNGAVATEMATYMRDRLSPEWRIYFFGPPRMYIAFGSIPYIAPDVQGVDISEPLTA
                     PLDPAIVQPDKNAAFVFLPERLGELEWVRKTFPNGALEEIPSSWNGDPIQAFVVYRVQ
                     IPSTQ"
     gene            complement(9953..10525)
                     /gene="mce"
                     /locus_tag="AC162G_15365"
     CDS             complement(9953..10525)
                     /gene="mce"
                     /locus_tag="AC162G_15365"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HIR5077233.1"
                     /translation="MPIKRIDHIAIVVDDIDASLVFWEDALGLKLSHLEDAKDQESLV
                     AFLPSGESEVELVEPTSESSGVARYLAKRGPGVHHICFEVDDIRMALARLKRHGVQLI
                     NEQPTIGTGGKQIAFIHPKSTGGVLVELYQLTPDEPRRRSKIIRDMRRRLVVQGYVAG
                     AGLREFIRVWRGRDGLRRKVETPDEKLRIN"
     gene            complement(10558..12219)
                     /locus_tag="AC162G_15370"
     CDS             complement(10558..12219)
                     /locus_tag="AC162G_15370"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR5077234.1"
                     /translation="MATSSNSKREWQKRAVEPFIERHGERKGRFATSSDIEIDRLYTA
                     EDRQHADDARDLGFPGEYPFTRGVQPTMYRGRLWTMRQYAGYASAAETNARFKYLLQQ
                     GQTGLSVAFDLPTQIGYDADDPMSEGEVGKVGVSISSLQDMLQLFDGIPLDQVSTSMT
                     INAPAAILLAMVIATGRRQGVEPKRLRGTVQNDILKEYIARGTYIFPPEPAMRLITDV
                     FRYCAAEAPHWNTISVSGYHIREAGSTAVQEVAFTLSNGIAYVQAAVDAGLDVDSFAA
                     QISFFFNAHNNFLEEIAKFRAARRLWAHIMRERFGAKDPRSWMLRFHTQTGGSTLTAQ
                     QPEINVVRVTLQALAAVLGGTQSLHTNSRDEALWLPTEESVRIALRTQQIIAYESGVA
                     DSVDPLGGSYLIEHLTDEIEQRAREYIERIDALGGALRAIETGFVQQEIADAAYACQQ
                     AVERGEQIVVGVNQFQIDEQPRLERLKIDPAIEFEQRRRLAELRRRRDNGRVADLRGQ
                     LDDAARSGADLLPLFVECVEHDVTLGEICGTLRDVWGEYRPATTT"
     gene            complement(12249..12806)
                     /locus_tag="AC162G_15375"
     CDS             complement(12249..12806)
                     /locus_tag="AC162G_15375"
                     /inference="COORDINATES: protein motif:HMM:NF014038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HIR5077235.1"
                     /translation="MKFRVPDLAALRDRLIAHGAIPIQPRHLERNVLFDDSEKSLRGR
                     GMLLRLRDALGAHITLKAPAPPDRQSSQHKARVEHEISVDSVDAAFAILTGLGYAPWW
                     RYEKYRESFRLDDATVALDHTPIGDFVEIEAPPESIRLVAGQLGFNWSARSLQTYREL
                     FDAAGHAAGVDMAFDTVQAGAETGK"
     gene            complement(12882..13379)
                     /locus_tag="AC162G_15380"
     CDS             complement(12882..13379)
                     /locus_tag="AC162G_15380"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HIR5077236.1"
                     /translation="MKYASTVAGQTYVIEINREGEITVDGVTRRADLRAIDGLGTYSL
                     LIDDESHEALVEARGGEIGVLLRGRFYLIQVQDERERRLAESTRGLVAPAGEISIKSP
                     MPGLIVAVRAEPGQGVKKGETLVILESMKMENELKAPRDGKVGVVRVQPRQAVEQGQT
                     LVTLE"
     gene            complement(13376..14878)
                     /gene="accC"
                     /locus_tag="AC162G_15385"
     CDS             complement(13376..14878)
                     /gene="accC"
                     /locus_tag="AC162G_15385"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR5077237.1"
                     /translation="MFQKVLVANRGEIAVRILRACRELGILTVAVYSEVDRSALHVRY
                     ADEAYCIGPAPSRDSYLRIDKILEVAHRSEADAIHPGYGFLAERSDFAEACERAGIVF
                     IGPKPSAIAAMGDKAVARAMVQKAGVPVVPGTEGEGGLRDEEIIAIAPHVGFPLLIKA
                     TAGGGGKGMRVVDDAEGLPGALASARREAEAAFGDGSVYLEKLVVGARHVEIQLLADT
                     HGNVIHLNERECSLQRRHQKIIEESPSMHVDGDLRARMSEVAVTAAQAVQYVNAGTIE
                     FLLDKDNSFYFLEMNTRLQVEHPVTEMVTGVDIVKEQIRIARGRPLRYMQDDIKINGW
                     AIECRITAEDPFDNFMPSTGRITRLAIPTGPGVRVDTGVYEGSEITPYYDPLIAKLIC
                     WGETRGEAILRMRRALEEFRIIGIKTSIPFHQAMMDSHRFMAGSFDTRFVEERFSLDE
                     EAHADPMRVAAIIGTLVAHGEGQKATQVVRAGERDTSNWKWLARWERLQR"
     gene            complement(14930..15316)
                     /locus_tag="AC162G_15390"
     CDS             complement(14930..15316)
                     /locus_tag="AC162G_15390"
                     /inference="COORDINATES: protein motif:HMM:NF017020.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR5077238.1"
                     /translation="MSVHPADEWVKKAEDNYISAVALLRRRSRPVPDVVCNQCQQCAE
                     KYLKALLVRHRIDFPKTHDLIQLKNMLVQADTDVQLMTSHLATLLPYAIDVRYPGLEA
                     AVEDAKEAVRAMKTIRRFARAKLGLR"
     gene            complement(15294..15710)
                     /locus_tag="AC162G_15395"
     CDS             complement(15294..15710)
                     /locus_tag="AC162G_15395"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR5077239.1"
                     /translation="MSTAGARRASQQARRAKRNGRKAPPSFYWWPITDAKIRRAAQRI
                     VAAVHPEKIILFGSFAYGKPTPDSDVDLLVIMESNLRPHARVVQISDILDPRPFPVDI
                     IVRTPSEIAERLRAGDCFFREILSKGRVLYERASRG"
     gene            complement(15733..16113)
                     /locus_tag="AC162G_15400"
     CDS             complement(15733..16113)
                     /locus_tag="AC162G_15400"
                     /inference="COORDINATES: protein motif:HMM:NF025341.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HIR5077240.1"
                     /translation="MRFSYDTNYFPPAPSVEISLGIPNESLAVGPLRAVVDTGADVLL
                     VPLRYIGPLGAQIDNRKTLRSQWGEPRKVDVYVLDVGIGDLRFPVIEVIADEVGDEIV
                     LGRNVLNKLTLTLHGPLQVLEILG"
     gene            complement(16110..16481)
                     /locus_tag="AC162G_15405"
     CDS             complement(16110..16481)
                     /locus_tag="AC162G_15405"
                     /inference="COORDINATES: protein motif:HMM:NF039192.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5678 domain-containing protein"
                     /protein_id="HIR5077241.1"
                     /translation="MSTVVLRDDLHETLKRNARQESRSVDEIVNEAVSLYLRERQRAK
                     LDKEIAAYEAMHAELRQRYLGQWIAVHEQQLVDHDPDGSALYQRIRSRYGKTSVLIRQ
                     VREQPSDDAYVRTPSSGKAAL"
     gene            complement(16571..18121)
                     /locus_tag="AC162G_15410"
     CDS             complement(16571..18121)
                     /locus_tag="AC162G_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015911221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR5077242.1"
                     /translation="MVVDPKIQKLRDTKEQARLGGGRERIDQQHQKGRLTARERLDLL
                     LDSGSFRELDMFVTHRATDFGMADKKVLGDSVVTGWGTIDGRLVYVFSQDFSVFGGSL
                     SQVHAQKVCKIMDMAMRNGAPIIGLNDSGGARIQEGVVSLGGYADIFLRNTLASGVIP
                     QVSAIMGPCAGGAVYSPALTDFIFMVNNTSYMYVTGPDVVKSVTHEEVTHEELGGATV
                     HNTKSGVAHFAAPSEADCLFMIRQLLSYLPQNNMEDPPLVRTQDDPLRMEERLDTIVP
                     DSPNKPYDMKEVIRLVVDEGRFFEVHEQFAMNIVVGFARLGGHSVGIVAQQPQVLAGV
                     LDIDASEKAARFVRVCDCFNVPLVTFEDVPGFLPGVAQEHGGIIRSGAKLLYAYCEAT
                     VPKITVITRKAYGGAYDVMSSKHIRGDLNLAWPSAEIAVMGPDGAVSIIFRKEIAEAE
                     DPVAKKAELVAMYREKFANPYVAAEWGYIDDIIEPKETRPRLINALEMLQNKRDSNPP
                     KKHGNIPL"
     gene            complement(18165..18563)
                     /locus_tag="AC162G_15415"
     CDS             complement(18165..18563)
                     /locus_tag="AC162G_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077243.1"
                     /translation="MKRPFRITLIVLGVLSLAVFNAWRAILAARQFEYLRGLSLEGPA
                     LALSLTGLVWAAGFGVAAIGLYRLRRWARLWTMAAIAFYTANLWLIRLIFEKSPDEPL
                     TRPADAALAVLSIAVVWGALSWPSLRRMFT"
     gene            complement(18567..19598)
                     /locus_tag="AC162G_15420"
     CDS             complement(18567..19598)
                     /locus_tag="AC162G_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR5077244.1"
                     /translation="MKALYFHEHGGPEVMQYGDLPDPEPGPGQVRVRVKAVALNHLDL
                     WVRRGGPTFERLPKPHIGGCDVSGIVDSHGEGAGGPPAGARVAIDPGLSTKEDEWTRR
                     GEDSLSPGYVILGEHSTGGAAEYVVVPASNLLPMPDHVSHEAAAAASLAGLTAWRMLV
                     HRARLKSGETVAVVGAGGGVNHLAVQVAKHLGATVYAITSSEEKMEKARALGADYAIN
                     YKAEDWSKAVFVATGKRGVDVVVDNVGQATWMQSIRALARGGRLVTVGNTGGWEAVTD
                     VRYVWSKQISIIGSTMGSHQDYRGVMQLVFEGKLKPVIHTTLPLSEGRVAHEILERGE
                     QFGKVVLLP"
     gene            complement(19595..20170)
                     /locus_tag="AC162G_15425"
     CDS             complement(19595..20170)
                     /locus_tag="AC162G_15425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HIR5077245.1"
                     /translation="MPTRVASYLSPKVEVREHADKGGYGVFTREPIAAGELLAVWSGQ
                     IVTLEQWEHLPPDIQRHTVQVEENLYLASLYPDEPADYINHSCDPNAGMSGQIAVVAM
                     RDIAPGEEVCIDHAMCDGTPYDEFDCQCSTPHCRRHVTGDDWRIPELWERYDGYFSPY
                     LQRRIERLKQQPAAWPGAILTTDDTDYEDLA"
     gene            complement(20308..21495)
                     /locus_tag="AC162G_15430"
     CDS             complement(20308..21495)
                     /locus_tag="AC162G_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR5077246.1"
                     /translation="MAIAELTHAGPNLELTEEHKMIRDAAREFAQKEIAPIAPHFDET
                     GEFPLETVMKMGELGLMGVEVPEEYGGAGLDTLAYVLAMEEIAKADVSHSTIMSVNNS
                     LYCHGILKFGTEEQKRKYLVPVASGKALGAYSLTEPMSGSDAGNMRSRAVRDGDHYVI
                     NGRKSWVTSGPVADALVLFTMTAPERRHRGITAFIVDTKQPGFVKGKTEPKLGIRASA
                     TCEIVYDNYRCPVENRLGEEGEGFKIAMHVLDAGRIGIAAQALGVAEAAFEASVQYAQ
                     TREAFGQPIGQFQGIQWMLADMKTRIEASRLLIWNAALAKERSKHDGQRYTLEASIAK
                     LHASETAMWVTEKAVQIHGGMGYSKEMPLERYFRDAKITEIYEGTSEIQRLVIARHVL
                     GLK"
     gene            21630..23228
                     /gene="ggt"
                     /locus_tag="AC162G_15435"
     CDS             21630..23228
                     /gene="ggt"
                     /locus_tag="AC162G_15435"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR5077247.1"
                     /translation="MKTAAFRFHTRRSVSMAMNGMVATSQPLAAIAGLRMLLRGGNAV
                     DAAVATAAALNVVEPTSTGAGGDVFALTYVARTGEVTALNASGCAPKAQTLDEMRRRG
                     HTAMPTYGGLAVTVPGAVAGWHDLLVRHGTMTLADVLQPAIDLAGQGFPVTEVIAGHW
                     SRLERRADQFPALAKTYLIDGHAPRPGEVFKNPELARTLRAIAEGGPDAFYRGPIAES
                     IVKIIQENGGLMTLDDMAAHASTWDEPIQTDYRGVTIVECPPNGQGIIALEALNILEG
                     FNLVAMGYGTPAYYHHLIEAAKLAFADAFAYVGDPRMAHVPIEGLIDKAYAAGRRQSI
                     DPARAASTPVPGRLRDGTVYLSVIDKERNACSFINSNYMGFGSGLVAEGVALQNRGAL
                     FSLEEGHPNCTTPGKRPYHTIIPALALKRGKLWMSFGVMGGFVQPQGHVQVACNTIDF
                     GMNPQQALDAPRVVWHDGALIDVEDGIGEDMRSALAKMGHDLMPKDDPRRHYGGGQIV
                     AIDEDSGALLGGTEPRKDGVAIGY"
     gene            23370..>24049
                     /locus_tag="AC162G_15440"
     CDS             23370..>24049
                     /locus_tag="AC162G_15440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HIR5077248.1"
                     /translation="MLSNLPPIWPVSEVVIFDCDSTLSGVEGIDELARMAGRQEDVSA
                     LTRRAMSGDVPLESVYEHRLAVSNPTQAQVSHLRRVYRETVIADAREVVAALQDLGCQ
                     VFVVSGGLIEPVRDFGAWLGVPRDHIYAVGMEYDQLAGEWWRYWEQPGGQNPNANYLA
                     VKSSPLVSTGGKRVVIDRIRQRFPGRAMLVGDGTSDLEARTQVDLFVGFGGVVHRERV
                     AAQAPIYV"
BASE COUNT         4231 a         7572 c         7713 g         4533 t
ORIGIN      
        1 gcgtgggtcg gcgcgaatgc gaaggagaac gtctgtccct gatacatgcg ggagcagctt
       61 caaatccaac acgaccaggt ccggcgctgt cgcagccagc cgctccaggg cggcttcgcc
      121 cgtccgaatg atttccgtcg taagcccgct cgactccagc acgcggctgt agagttctgc
      181 cacatcctgg tcatcttcga cgattaacac agaagggtcg ttcatgatgc ggtctccttc
      241 ccccgaatgc cgctagggcc attcgagact cagcccgccg aggcgagcgc ctgactctca
      301 aatgaggtag aatgcccgtt gtggtgggtg atcaagaatc ttctgcagct catccgccag
      361 cagtgtcaca ccggtgtcgg tcattaccct gataatagtc tatggcaaca caccccctaa
      421 gtcaagtgtg cgtatggtca tgacagcggc gccctgtggc cggacggcgc tcgcgggcct
      481 ttgcgccgcg agccggcaga gccgtgcact cggctccgag gccaggatgc tgctgaccta
      541 tttcgtcgcc gtgacgcata cgccgtccga aatcgaggag tggtggacgg ttgtcgagga
      601 gcatcggctt gacgctcatt gccaggcaag gtatactgcc atcaggttac gggcccatag
      661 ctcagcggtt agagcagccg gctcagcgca gtgaatctga agggctgcct ggcgagcaat
      721 cgccaggtga tacctgccaa attcggggaa gcctaagtcg caagataggg taatcccgag
      781 ccgagcctca tacgtgagga aggtgtagag actagacggc aggcgcccca ccggggtgaa
      841 gggatagtcc agcccacaaa cccgaaaggg gcagcgaaag ctgtagtggg acgcataacc
      901 ggtcggtcct tggttcgaat ccaagtgggc ccatgtgaca cgacccgatc acgccgatcc
      961 ggcggatcgg gtcgtcgtgt tttctgcggc agaaacctgc gccgggtgct ggacgtacac
     1021 ggtgcgaatc gggaagggga tgtcgatgcc ttcgcgccgc gcagcgtcta tgatggactg
     1081 cgtgacatca gtctgggcgt caactggatt agtggcttgc caatcgatcc agaagcgaaa
     1141 taccatccgg atcgagctgt cgtcgaaggc ttcgaacgag atcagcggct ccggctcggc
     1201 gagcacgcca ggggtggaca cgacggcctc ttttagcaca cggtacgcct tgggcagatc
     1261 ggtatcgtaa gcgacgccca ccgtcgccgt gattcgccgc gtggggtagt agctccagtt
     1321 cgtgatgact tcgttgtaaa cctgtctatt tggaattaag acgtgcaagc cgtcgaaggt
     1381 tttgacattc gtggtgcgaa tggcgacgtc ggatacgacg cccatgacgt cgccgacttt
     1441 gatgatgtcg ccgaccttga acgggcgctg aatgagcaag agcacgccgg tgatggcgtt
     1501 ctcgatcgtg tccttgagcg cgagaccgat ggccaggctg cccagcccga gcgcggcgat
     1561 cacggcggcc acatctagac ccatcgtcgc cagcgccgtc agcgcggaca gcgtgacgcc
     1621 gataatgccg accacgcgcg acatcgctcg ctcgacggtc aggccggccg gcgtattatg
     1681 aatcgcgcgc acaatgattc gttgcatcac acgcgtgagg acgtagccga tggcgaagac
     1741 gatcagaccg atcacgatgc tctgtataag gacgggaaag tcctggccca tgcgcgccag
     1801 tgcctgctcg atcaactcga ttaggtccat gtcatctccg cttgaacagg tcgagccatt
     1861 cgtcgatgtc cccctcgacg ccgggcttct cgtcggggtc ctcgcgcacc tgttccgcgg
     1921 agccacgtgt cggcgccagt tggccggcga agtgggccga ttcgacgacc tgcaagcgcc
     1981 gccggcgcgc ctcctgttga atcgcgcgat cgctggagac aagcttccat tgctgggggc
     2041 gcttcagctc gcggatgcgc tcgatcagga tgcgatcggc gatcgtaccc gagccggcga
     2101 agatggcttc gacaccgccg ccgctcagtt cgcgcgaccg gccggcgagg ttgcccgagt
     2161 cgaacacgac gacgacgtgc gcacggtgct gggctgcata tttgcgcagc aagccgacga
     2221 gcttctcttc gtcgtcgatc tgggaaaggg aaaggccagg aattctcggg atcaggttgt
     2281 ggccatcgat cagcaactga atcatcgcgc agaggacttt tggacgctga ttcacgcgga
     2341 tgaacgccga tggagagttc ggatcagcgg gaatctgcgt tcatccgcat cccaataatc
     2401 acttgtggcg gagccgtgct gtgacttaac ccggcagctt ctccaggttg gcgaagctgg
     2461 ccaggagccg cttgatgccc gctttctcga acgccacggc cagctcttcg tcgtcgcctt
     2521 gcacccggct ctccacgacg acgccttcgc cgaaacgggc atgcttcacg cgctggccgg
     2581 gccggaaggc cggctcgcgc gacgggcggg ccggcggcga ggagcgttgc gctgaggccc
     2641 acgtcgccgc gcgctgcgcg gcggcggcgc gcggcgtggc gcccgcgaat gacatggacc
     2701 cgctcagcaa ggcagtgggg atgtcgctca ggaagcgcga cggcgcgttg acctcactgc
     2761 tgccgtacgc ggtgcggcga aacgcgtagc tcaggtacac ccgatcctgg gcgcgtgtga
     2821 tgccgacgta ggccagacgc cgctcctctt ccatctcgtc cgggtcttcg agcgagcgcg
     2881 cgtgcggcag gacgccttcc tccaggccgg tgatgaagac gaccgggaac tccagcccct
     2941 tggccgaatg cagcgtcatc agcgtcggtg cgtcgacgcg ctgcgtgtcg tcgagcgagt
     3001 cgacgtcgga cacgagcgcc acatcctcca ggaattcggc cagcgggtcg ccgccgacgg
     3061 cgtgtgtgta atcgctcgcg accttgcgca gctcgcgcac gttttcccag cgctcgcggc
     3121 cttcttcgct gccgtcgtcc acgtaggcgc gatagcccga gtccttgacg acggcgtcga
     3181 gcaggtcggc caccgagccg tatcgatcct tcttggcgat ccagcgatcg agcagggtcg
     3241 cgaaatcgac gagcgcctgg cgcgcttttc cggtcaggct cgacacgagc gccgggttgc
     3301 cgcgcccggc tctcagctcg gcgatcagcg tgtaggggct ttgacgggtc gcgcgcgcgg
     3361 ccccgtcgag cgcggcgagc gtcttcgcgc cgacgccgcg cggtggcaca ttgacgatgc
     3421 gcgccaggct gacggcgtcg tgcgggttgt ggaccaggtg caggtgggcg agcacgtcct
     3481 tgatctcgcg ccgcgcatag aagcgcgtcc cgccgaccag ccggtagggt acgcccgccc
     3541 ggatgaaagc ctcttcgaga gggcgggact gcgcattcgt gcggtagaac acggcgaagt
     3601 cggcggcttt atactgccgg ctgcggatga gatcctcgat cgtgttgacg acgtacagcg
     3661 cttcgtcgtc ctcgttgtag gcttcgtaga tcgagagcgg cgcgccgccg ctgcgctcgg
     3721 tgaacaggcg cttgtcggtg cgctggcgat tgcggcggat caggccgcgc gccgcgtcga
     3781 ggatcgtctg tgtcgagcgg tagttctgct cgagcaggat gacctgcgcg tcgggatagt
     3841 cctgctggaa gcgccgaacg ttgcggaagt ctgcgccgcg aaaacgataa atggagttat
     3901 ggacgaccac gcctcccgcc agatagttgt ggaggtgctg gacttcgaga tcgtacactt
     3961 tgcccttata cgcttcgcgg tcaatctgga caatttcgtc ctgaacgatg cgtccctggc
     4021 gctcggcggc gacgatcatt gtagggtgca gatggctgac gggcatcaag ctgaatcgcc
     4081 gagcgatctc atcgcttgag gattcaacca gaaatgcgcc gacgctgatc tccaggttcc
     4141 cacacacccg actgatctcg cctgcaaggg cttgcgcgtc atcgaagttc agccgcgaga
     4201 actcggcgcg ccacgtgttg ccccctcctc tacgggtgct gtaaccgcgc gcctcaagtt
     4261 tgcgtcgcaa agcggggtct tcgcaattca cactcacgcg gtgagcgttc cagggtgttc
     4321 tttcactgcg acgtgagtcg ctgaaataac gcagattcac gacgtgccgc aactctttgg
     4381 ccttggggat atagtgcggg tagcgagtgt ccatcatcag atcggccagc aagcgttcgg
     4441 cgcgttcgcc tgtgtcgata tcctgataga gcctgtcgac ggtcacctgg gacatacgta
     4501 aacgccgccc ggcaacgtga aagacggtcg tggggattcc gtactggaaa gcgaggaagg
     4561 cttcccagta tagtgcttca tcgcgactcg aacaggctcg tagaatccag acccgatctg
     4621 cctgctcctg attgcctcgc accctcagtc ccgattgcca ttccggcctt acgccgtcgc
     4681 ttctcgcgcc gctggtcacg ccgatgcgat acccgacgcc ctccttttgc atgaggtaaa
     4741 cgtagtgtat tccggccgag gcgctcaggc gggcgaagac gatatggttg ggggtcgcgc
     4801 ggaacgagta accttgccgt gtcgtgacgc gaatgagttc gccgcgatag ggtcggcttc
     4861 caacgtacac cacctcagtc ggcagcgtag cgccgcgccc gctcgcagcc aggactttct
     4921 ctccgatcct gatcgtctcg ataggcttgc ggccacttgg cgtttgaatc gctgccccgg
     4981 ctgttaggca ctgatcctcg tctcctacga caaacaggtt gcgcgaccgg ccggccatct
     5041 gccgcagcag cgtgtactgg gccgtattgg tgtcctggaa ctcgtcaacc aggatatatc
     5101 gatagcgatt ctggtagtag ccgagcacga ccggattcgc gtcgaagagc tggacgacgg
     5161 ccatcaagag atcgtcgaag tcgagcgcgt tgttcgcgcg cagcaggtcc tggtagtggg
     5221 catacacgcg cccgacggcc tcgtgccagt acgtccgcgc gacgtaggct ccgggcggga
     5281 tcagctcgct cttggccgcc gagatggcgt tgagcacgga agacggcttg tatttcttgt
     5341 cgtcgacgtt caggtccttg agcgcgcgct tgacgagcgc ctcctggtcg tcggcgtcgt
     5401 agatcacgaa gtcacggctg atgccgaggt gcggcgcgtc gcggcgcagg aagcgggcac
     5461 agttggcgtg aaaggtgccg agcgtcagct cggctgtccg gttcgggccg agcaggcgat
     5521 ccaggcggtc tttcatctcg cgcgccgcct tgtttgtgaa ggtcaccgcc atgatatggc
     5581 gcggatcgac gccgcgcgct tcgatcaaat aggccacgcg gtgcgtcagg acgcgcgtct
     5641 tgcccgagcc ggggccggcc aggatcagcg caggcccatc acccgcctcg acggccatgc
     5701 gctgttgagg attaagtccg cgaagccagt tcattcggtt cacctgtgag aattaaagat
     5761 cattgtacca catgcggatg agaggtttca tcacccgccg cgcgtgccca aaacatcaag
     5821 ggcaacttgt tgctccgaca agttgccccg gggtgagctg atgcgccgtt gcggctagaa
     5881 attcttcaag acgatgggca ggttgatgcg gtagccgaac ggcggataat actcgtccgc
     5941 gccgatgtcg aagccgctcc cctgcggtcg cgtatcgccg tcaatgtcgg tcgtgactcc
     6001 tacgccgatt cccttgtcga tagccgcgga gccagcgctc aggtggtagt cgtcgccagc
     6061 gggatcggcg aacctgggat cgccggtctg gtcgtgcgcg ccgccgccga tcgtgccggt
     6121 tttgccgatg gtgttgccgt agaacaggtt gtagtcggcg tacaccgtgc tatctgtttg
     6181 gctaatgccg gcagtatggc ttgcgatgat ggagttggtg atgccgaccg tgccgctgac
     6241 gacgacaata gcctgcttcg ggttgaggct cccatcggcg acggtcgtgt gcaagatgac
     6301 cacgtcgccg cctgagccaa gccgcaaggc cgcgcccccc gcgccggcgg tattccgtgc
     6361 gaagagcgcg ttgacaatcc ggccactgcc gatggggcct tcgaaaacac ttgagtggaa
     6421 aactccgccc ccacttcctg gcgtcgcatt gctgatgaac tgagtcccac tgatggtcaa
     6481 ggcatcgaaa gcgtccatgc cgccaccgaa ccctaaccca aaggtcagac caacccggtt
     6541 gttctgaaag atgcccccac ttagcgtgat agggccgtct gcgcgcatcc caccgccgcc
     6601 gaattccgct gtgttgctcc ggaaatcggt gtccctaagg acaactgtgc cacgtgtgta
     6661 gagaccgccg ccgacgtact ttgcggcatt gcgctcgaac gtggcgccag tcaatgaggt
     6721 tgatcctagc acagaggcgc ctccgccgtg ctgcgccgag ttactgatga actgcgttcc
     6781 gcggagggtc aatgtacccg acacgctcag cccgccgccg agactcgtcg taggattcga
     6841 gctggtgctg tggttgttct cgaattcgcc tgccgtgact gtggccgtcc cgatcacgta
     6901 cgcgccgcca cctctgccga aggctgtgtt gctgacaaac accgtcccgc tcaaggcaag
     6961 cgtgttgctt acgtataccc cgccgcctgc tgacccatcg ttttgctcga accgaccgcc
     7021 gatgagcgcg acagaactga gtgcgtataa tccgccgccg ttgccgaaat ctgagacgtt
     7081 cctggcaact gtcacattcg tcagggtcat caggccgctt gcgtcaatat acagcccacc
     7141 gccgtggaag tccgacctgt tgtcaacaat ggtgctgttc tgaatcaagg ggcgagccat
     7201 gccggtgatc agaacaccgc caccacagcc cgccgggcag gggaagccgg tgaggttccc
     7261 gcccgcgaag gtcaggccgg agatgatcgt cgtcgcagtg acagtgctgc ccgtgatcaa
     7321 catggcccga tcgtcgatga cggcgtgaac gatggtcgtg cctgcatccg cgccgatcag
     7381 gctcaccgcc ttgttgagta caaggctctc ggtgtacagg ccggccggga tcaggatacg
     7441 gtcgccggcg acgacaatcg ggttgtcaat gcatgcttga atcgtggccc cgcacggccc
     7501 gccgacggtg taatcgttgc cggacacaga tcgatagcgt tcggagtcag ccgctttagc
     7561 ctgtatagca gacgtctgac ccaatatcga ggcgaaaaca gacagcagca ggatcgccag
     7621 cgagagcgca acgaggcatc gcacggacgg ccgtgtggtc atggttctct ccatgtggaa
     7681 acggattatt gagtcgaagg tatctgcacg cgatagacga cgaaggcctg gatcggatcc
     7741 ccgttccagg atgatggaat ctcctcgagc gcgccgttgg ggaaggtctt ccgcacccac
     7801 tccagctcgc cgaggcgctc cggcaggaaa acgaacgcgg catttttgtc cggctgcaca
     7861 atcgccggat cgagcggtgc cgtgagtggt tccgaaatgt ccaccccttg gacgtccggc
     7921 gcgatgtagg gaatcgatcc gaacgcgatg tacatgcgcg gcggcccgaa gaaatagata
     7981 cgccattccg ggctaagccg gtcgcgcatg tacgtagcca tttcggtggc cacggcgcca
     8041 ttcaagtcgc cgtacaggcg cagcggcgtg aactccacaa agtaccactg cacgctggcg
     8101 ataatgataa cgagcatcga agggatcaga tacaatgcga agatgtgtgg cgtccaggcg
     8161 tcccacacgc tccggaggat ctggccgatc ttgagcagcg ccagcgcgac gaagaagacc
     8221 gccggcggcc cgagcgtgac cagccgctgg ctggacggcg gtccttgcgt cagcgcgccg
     8281 ccgaggatca tcgctcccca ccaccaggcg accatcggga agagccgtcg atcgccgagg
     8341 cgcagtgtgg catagcccag cccgagcaga aagaggacgc cggtcgcgtt atcgaacagc
     8401 ggccgtggcg agccgtacca gctcgttcga tccgggtaca cgttgaaagc cagcgcggcg
     8461 cgccgaagct ggtcgagcag gatcgagact tcgtcttgac cggtgagctc ctgcgcacgc
     8521 tgcagccagc ctgtctgaaa gatgctgatc tggttgacgc gcgcgttgta gtcctgcggg
     8581 tagcggatcg cgaattgaat catcggcgcg ccggcgacga tcgccgcgcc ggccatgatg
     8641 acaatgcccc cgccgtgcac gcgcaggaag tgacggccat cccgggcgac gaaatagacg
     8701 atcatcgcac cgaccaggac gggggcgaag cgcgcgccga aatagaagta ttgcgccacg
     8761 ccgacggcga cgccgcacag cgcccactcc agcacgctgc gctcgtcgta ggctcgatag
     8821 agtaagtaca ggacgagcgc gatcagcaat gggtcggcgg cattgttgac gcccaggcgc
     8881 gagaaatgga tgtggaagtg gtacgtcgcc aggagtgccg ccgccatcag ggcgagcgcc
     8941 gcaccggtga gacgacgcac taactggaag acgattacta cggtcagcgt ccccaccagc
     9001 gcccacggca gccgcagcgc gaacaccgtc tggcccagca tgcggatcgt aagcgagttg
     9061 aagtagaagc tcatcgtcgg cacgctcagc cagcccgtgg taaacgggtt gcggatctcg
     9121 ccggtgaagg ttctgatcga ctccagccca aacgtgccct cgtcgccgcc gagtgtgggc
     9181 gggatacctt cgatgttcca gacgcgcagc gcgaacgcgg cccacacgat ggccgccagg
     9241 atgaggaagt gcggccagtg agccttgagc cacgacccgg tatgggggcg tgtcggcagg
     9301 ggccacggca cgaccgcgcg aatgtacagg ccgatcgcgc tcagccacag aaggaatggc
     9361 agcgtgtagc tgggcagcga cggatccgct ctcagcagat cgaggaggat gatcgtcaac
     9421 agcgccactg cgccgagcag ggcaatgcga cgcggctggc ggcgcagcga tgtgaagacc
     9481 ggttggcttt cgtcaacctt aggttcacgc gtgggttcga cttgcgaatg gcgttcagac
     9541 gcgcgccacg tctccgccgt ctctacggcg acgccggccc atgcggcggc gacggtgaac
     9601 agcacaatgg ctatcgaaaa cagggcgact ccggtggaca ggttggggga atccggccct
     9661 tcggtgaaga cggcctgtcc gagcagcgcg aggaccaacg cggcgccgag caggggcagc
     9721 gtgagccggc gctgcatccc ggcaggaagc gggagagaca cgactgcggg cgtcagcttc
     9781 tcgacgggcg cggccggggg cgcgagcgcg ccggatcgtg tgtccggtgt tatcggtcga
     9841 cgtttgctgc gctgccagag ctcgagtgcg acggcggtga ggagcgcaat cccagcgtac
     9901 aggacgtacc gtgaccgttt catgcgcgtc agtagaccca gcgtatgcgc cgtcagttta
     9961 tacggagctt ctcgtccggc gtctcaacct tgcgccgaag gccatcacgg ccgcgccaga
    10021 cgcgaatgaa ctcgcgcagg ccggcgcccg cgacgtatcc ttgcacgacc aggcggcggc
    10081 gcatgtcgcg aatgatcttg ctgcggcggc gaggctcgtc aggcgtcaac tggtacagct
    10141 cgacgagcac gccgccggtg ctcttggggt ggataaaggc aatttgcttg ccgccggtgc
    10201 caatcgtcgg ctgttcgttg atcaattgga cgccgtgacg cttcagcctg gccagcgcca
    10261 tccggatatc gtcgacctcg aagcagatgt gatgcacgcc cggcccgcgc ttggccaggt
    10321 atctggcgac gccgctggat tcgctcgtcg gctcgaccag ctcgacttcg ctttcgccgg
    10381 aaggcaggaa ggccaccagc gattcctgat cctttgcatc ttcaaggtgc gatagcttca
    10441 ggccgagagc gtcttcccag aagacgagcg acgcgtcgat gtcgtcgacg acgatggcga
    10501 tgtgatcgat acgcttgatc ggcatgtggc tcctgtcgcg ggacggcagt cgcgtagtca
    10561 ggttgtcgtc gctggcctat actcgcccca tacatccctg agtgtaccgc aaatctcgcc
    10621 gagtgtcaca tcgtgctcga cgcactcgac gaagagcggc agcaggtcag cgccgctgcg
    10681 cgcagcgtcg tcgagttgtc ctcgcagatc cgccacccgg ccgttgtcgc ggcgacggcg
    10741 cagctcggcc aggcgccgcc gctgctcgaa ctcgatggcc ggatcgatct tcaagcgctc
    10801 caggcgcggc tgctcgtcga tctgaaactg gttcacgccg acgacgatct gctcgccgcg
    10861 ctcgaccgcc tgctggcagg cataggcggc gtcggcgatc tcctgctgga cgaagccggt
    10921 ctcgatcgcc ctgagcgcgc cgcccagggc gtcgatgcgc tcgatgtact cgcgggcgcg
    10981 ctgctcgatc tcgtcggtca agtgctcgat caggtacgag ccgcccagcg gatcgacgct
    11041 gtcggccacg cccgactcgt aggcgatgat ctgctgcgta cgcagcgcga tgcggacaga
    11101 ttcctcagtc ggcagccaca gcgcttcgtc acgcgagttc gtgtgcagcg attgggtccc
    11161 gcccagcacg gcagccagcg cctgcaaagt cacgcgaacc acgttgattt cgggctgctg
    11221 cgcggtgagc gtcgagccgc cggtctgtgt gtggaagcgc agcatccacg agcgcgggtc
    11281 tttcgcgccg aagcgctcgc gcatgatgtg cgcccacaga cgccgcgcgg ccctgaactt
    11341 ggcgatctcc tccaggaagt tgttgtgcgc gttgaaaaag aacgaaatct gggcggcgaa
    11401 ggaatccacg tccagtccgg cgtccacggc ggcctgcacg taggcgatgc cgttggacag
    11461 cgtgaatgcc acttcctgca cggccgtgct gccggcctcg cggatatggt agccggagac
    11521 gctgatggtg ttccagtgcg gcgcctcggc tgcgcagtag cggaacacgt ccgtgatcaa
    11581 gcgcatcgcc ggctcggggg gaaagatgta cgtgccgcgc gcgatgtatt ctttcaggat
    11641 gtcgttctgc acggtgccgc gcagccgctt cggctcgacg ccctgccgcc tgcccgtcgc
    11701 gatgaccatc gcgagcagga tggcagccgg cgcgttgatc gtcatcgagg tggagacctg
    11761 gtcgagcggg atgccgtcga acagttgcag catgtcttgc agcgacgaaa tgctcacgcc
    11821 gaccttgcca acctcgccct cggacatggg atcgtcggcg tcgtacccga tctgcgtcgg
    11881 cagatcgaac gccaccgaca ggccggtctg cccctgttga agcaagtact tgaagcgcgc
    11941 gttggtctcg gcggccgaag cgtatccggc atattgccgc atcgtccaca accggccgcg
    12001 atacatcgtc ggctgcacgc cgcgcgtgaa ggggtactcg ccgggaaagc cgagatcacg
    12061 cgcatcgtcg gcgtgctgcc tgtcttcggc cgtgtagagg cgatcgatct cgatgtccga
    12121 cgacgtggca aatcgcccct tgcgctcgcc gtgcctctcg atgaaaggct ccacggcgcg
    12181 cttctgccac tcgcgtttgg aattcgatga ggtagccata gtgtgagaca tccgaagtct
    12241 tgctgtcgct attttccggt ctctgccccc gcctgcaccg tgtcaaatgc catgtcgact
    12301 ccagcggcat gcccggcggc atcgaatagc tcgcgatacg tttgcaggct gcgcgccgac
    12361 cagttgaagc ccagttgccc ggcgacgagg cggatcgatt cggggggcgc ctcgatctcg
    12421 acgaagtcgc cgatgggtgt gtgatcgagc gccaccgtcg cgtcgtccag ccggaacgat
    12481 tcgcgatatt tctcgtagcg ccaccacggc gcgtagccaa gccccgtcag gatggcgaac
    12541 gccgcatcga cactgtcgac ggagatttcg tgctcgacgc gcgccttgtg ctggctgctt
    12601 tgccggtcgg gcggcgcggg agctttcagg gtgatgtgcg cgccgagcgc gtcgcgcaga
    12661 cgcagcagca tgccgcgccc gcgcaggctt ttctctgagt cgtcgaacaa gacgtttcgc
    12721 tcgaggtggc gcggctggat ggggatcgcg ccgtgcgcga tgagacgatc acgcagcgcg
    12781 gcgagatcgg gaacgcggaa tttcacttcg gtctcaaggg ttgcgctcgt gcatgactcc
    12841 ttcggagcaa tgggggcggc ctctccgtga gccgcgccgt ctcattccag cgtgacaagc
    12901 gtctgcccct gctcgacagc ctggcggggt tggacgcgca ccacgccgac tttgccgtcg
    12961 cgcggcgcct tcagctcgtt ttccatcttc atcgattcga ggatgaccag cgtctcgccc
    13021 ttcttgacgc cctggcccgg ctcggccctg acggcaacga tcaggcccgg catgggcgat
    13081 ttgatcgaga tctcgcccgc cggcgcgacg aggccgcgcg tcgactcggc cagccgccgt
    13141 tcccgctcgt cctgcacctg gatgagatag aaccgcccgc gcagcaggac gccgatttcg
    13201 ccgccgcgcg cctcgacgag cgcttcgtgc gattcgtcgt cgatcaggag ggagtacgtg
    13261 cccaggccgt cgatggcgcg cagatcggcc ctgcgcgtca cgccgtcgac cgtgatctcg
    13321 ccctcgcggt tgatttcaat cacgtaggtc tgcccggcga ccgtgctcgc gtatttcatc
    13381 tttgcaggcg ctcccaacgc gccagccatt tccagttgct ggtatcgcgc tcgccggcgc
    13441 gcacgacctg cgtggctttt tgtccttcgc cgtgcgcgac cagcgtgccg atgatcgccg
    13501 cgacgcgcat cgggtcggcg tgggcctctt cgtccagcga gaagcgctct tcgacgaagc
    13561 gtgtatcgaa cgagccggcc atgaagcgat ggctgtccat catcgcctgg tgaaagggaa
    13621 tactggtctt gatgccgatg atgcgaaatt cctccagcgc gcgccgcatg cgcaggatgg
    13681 cttcgccgcg cgtctcgccc caacagatca gtttggcgat caagggatcg taatacggcg
    13741 tgatctcgga gccttcgtac acgccggtgt cgacgcgcac gcccggaccg gtcggtatgg
    13801 cgagccgcgt gatgcgaccg gtggagggca tgaagttgtc gaacggatct tcggccgtga
    13861 tgcggcactc gatcgcccag ccgttgatct tgatatcgtc ctgcatgtag cgcagtgggc
    13921 ggccgcgcgc gatgcggatc tgctccttga cgatatctac gccggtgacc atttccgtga
    13981 ccgggtgctc gacctgcaag cgtgtgttca tctccaggaa gtagaagctg ttgtctttat
    14041 cgagcaggaa ctcgatggtg ccggcgttga cgtactgcac ggcctgcgcc gctgtgaccg
    14101 ccacctcgct catgcgcgct cgcagatcgc catccacgtg catcgaaggt gattcttcga
    14161 tgatcttctg gtgtcgccgc tgcaggctgc actcgcgctc gttgaggtgg atgacgttgc
    14221 cgtgcgtgtc ggccaggagt tggatctcga cgtgccgcgc gccgacgacc agcttctcca
    14281 ggtagaccga cccatcgccg aaggccgctt cggcctcgcg ccgcgccgag gccagcgccc
    14341 cgggcaggcc ctcggcatca tcgacgacgc gcatgccttt gccgccgcca ccggcagtgg
    14401 ccttgatgag gagcgggaat ccgacgtgcg gcgcgatggc gatgatctct tcgtcgcgca
    14461 gcccgccttc gccttccgtg ccagggacga cgggcacgcc cgccttctga accatcgcgc
    14521 gcgcgacggc cttgtcgccc atggcggcga tagccgacgg cttcggcccg atgaatacga
    14581 tgcccgcccg ctcgcacgcc tccgcaaagt cgctgcgctc ggccagaaat ccgtaacctg
    14641 ggtggatggc gtccgcctcc gaccgatggg ccacctccag aatcttgtcg atgcgcagat
    14701 acgaatcgcg cgagggagcc gggccgatgc aatatgcctc gtcggcatag cgcacgtgca
    14761 gcgcgctgcg gtcgacctcg gaatacacgg ccaccgtcag gatgcctagc tcgcggcagg
    14821 cgcgcaggat gcgcacggcg atttctcctc ggttggcaac taagactttc tggaacatag
    14881 agttagacct aagcagaatc agcgagtcag cgaatcaacg ggacgctggc tatctcagcc
    14941 ccaatttcgc gcgcgcaaat ctgcgtattg tcttcatcgc acgtacggct tccttggcat
    15001 cctcgactgc ggcctcgagg ccgggataac gaacatcaat tgcatacggt aggagcgtag
    15061 ccaggtggct tgtcatgagt tgcacatctg tatcggcttg caccagcatg ttcttgagct
    15121 gtatgaggtc atgcgtcttt gggaaatcaa tccgatgccg aaccagaagc gccttcaagt
    15181 atttctcggc gcattgttgg cattggttac agacaacatc cggcacaggc cggctgcgcc
    15241 gacgtagcaa tgcgaccgcg ctgatatagt tgtcttctgc cttcttgacc cactcatccg
    15301 cgggatgcac gctcatagag caccctgcct ttgctcagta tttcccggaa aaagcagtca
    15361 ccggctcgca agcgctctgc aatttcagaa ggcgtccgaa cgatgatgtc cacaggaaac
    15421 gggcgtgggt caagaatatc agagatttgg acgactctag cgtgtggcct gagattgctt
    15481 tccatgatca ccagcaaatc cacgtcgctg tcgggtgtcg gcttgccgta cgcgaatgag
    15541 ccgaatagga tgatcttctc gggatgaacg gccgcgacga ttctttgggc ggcgcgacga
    15601 attttcgcgt ccgtgatcgg ccaccagtaa aatgacggcg gtgcttttcg tccattcctt
    15661 ttcgcccgcc gcgcttgttg agatgctcgc ctagccccag cagtgctcat gtcagactcc
    15721 cgcgccctcc tactacccga gtatttcgag cacctgcagc ggcccgtgca gtgtcaaggt
    15781 gagcttgttc aggacattac ggcccagaac gatttcgtcg ccgacttcgt cggcaatgac
    15841 ttctatcacc gggaaccgta agtcgccgat ccctacgtca agcacataca cgtcgacttt
    15901 tcgtggttca ccccactggc ttcgcaaagt cttccgattg tcgatctgtg cgccgagcgg
    15961 cccgatgtac ctgagcggca cgagcaacac gtccgcgccg gtatccacga ctgctcgcag
    16021 tgggccaacg gcgagagatt cgttcggaat accaagcgaa atctcgacgc ttggcgcggg
    16081 tgggaaatag ttcgtgtcgt atgaaaatct catagcgcgg ccttgcccga actgggtgtt
    16141 cgtacgtagg catcatctga gggctgctct cgcacttggc ggatcaggac cgacgtcttg
    16201 ccatatctcg atcgaatgcg ctgatagaga gcagatccat cggggtcatg atcgaccagc
    16261 tgctgctcgt gtacggcaat ccactggcca agatacctct gccggagctc ggcgtgcatc
    16321 gcttcgtagg ccgcaatctc cttgtcgagt ttggctcgtt gacgctcgcg cagatatagg
    16381 ctcaccgctt cattcacgat ttcatcgacg cttcgtgatt cctgccgggc gtttctttta
    16441 agggtctcgt gcaagtcatc ccttaataca acagtgctca tgccagcctc cttaaacgat
    16501 tacagaccac tccgggcacg agattgcgac tttcgcccac gcggcttgct cccggaaggc
    16561 gcaacatttg ctagagtgga atattcccat gcttcttcgg cgggttgctg tcacgcttgt
    16621 tttgcagcat ctccagcgcg ttgatcaggc gcgggcgggt ttccttcggc tcgatgatgt
    16681 cgtcgatata gccccactcg gcggcgacgt aagggttggc gaacttctcg cggtacatcg
    16741 ccaccagctc cgccttcttg gcgaccgggt cttctgcctc ggcgatctcc ttgcggaaga
    16801 tgatgctaac cgcgccgtcc ggtcccatga cggcgatctc tgccgacggc caagccaggt
    16861 tcaggtcgcc ccggatgtgt ttcgagctca tcacgtcgta ggcgccgccg tacgccttgc
    16921 gcgtgatgac ggtgatcttc gggacggtcg cttcgcagta ggcgtacagc agcttggcgc
    16981 ccgagcgaat gataccgcca tgctcctgcg cgacccccgg caggaacccc ggcacgtcct
    17041 cgaaggtcac gagcggcacg ttgaagcaat cacagacacg gacgaagcgc gccgcctttt
    17101 cgctggcgtc gatgtcgagc acgcccgcca gcacctgcgg ctgctgggcg acgatgccga
    17161 cgctgtgccc gcccagccgc gcaaagccga cgacgatgtt catcgcgaac tgctcgtgca
    17221 cttcgaagaa cctgccctcg tccacgacca gccggatcac ctctttcatg tcgtaaggct
    17281 tgttggggct gtcgggcacg atcgtgtcca gccgctcttc catgcgcagc ggatcgtctt
    17341 gcgtgcggac gaggggcggg tcttccatgt tgttctgcgg caggtaactc agcaactggc
    17401 ggatcatgaa caagcagtcg gcttcgctgg gggcggcgaa gtgcgccacg ccgctcttgg
    17461 tgttgtggac ggtagccccg cccagctcct cgtgcgtcac ctcctcgtgc gtcaccgatt
    17521 tgacgacgtc cggtccggtg acgtacatgt acgaggtgtt gttcaccatg aaaatgaaat
    17581 cggtcagagc cggcgagtag accgcgccgc ccgcgcacgg ccccatgatc gcactgacct
    17641 gcgggatgac gccggaggcg agcgtgttgc gcaggaagat gtcggcgtag ccgccgagcg
    17701 acacgacgcc ctcctgaatg cgcgcgccgc ccgaatcgtt cagcccgatg atcggcgcac
    17761 cgttcctcat cgccatgtcc atgatcttgc agaccttctg ggcgtgcacc tggctgaggc
    17821 tgccgccgaa caccgagaag tcttgcgaga agacgtatac cagccggccg tcgatggtgc
    17881 cccagccggt gacgacgctg tcgcccagga ctttcttgtc ggccatgccg aagtcggtgg
    17941 ctcgatgcgt gacgaacatg tccagctcac ggaacgagcc ggagtcgagc agcaggtcga
    18001 gccgctcgcg ggcggtgagc ctgcctttct ggtgctgctg gtcgatgcgc tcccggccgc
    18061 cgcccagtct cgcctgttcc ttcgtgtcgc gcagcttctg aattttcgga tcgactacca
    18121 tatcttgctc cgcactccag cccgcttgag tggcgccgcg attgtcaggt gaacatccgg
    18181 cgcagcgacg gccacgacaa ggcgccccag accacggcga tgctgaggac agcgagagcc
    18241 gcatcggcag gccgggtgag cggctcgtcc ggcgacttct cgaatatcag gcgaatcagc
    18301 cacaggtttg ccgtgtagaa cgcgatcgcc gccatggtcc acaggcgcgc ccagcgccgc
    18361 agtcgatata ggccgatcgc cgccacgccg aacccggctg cccacacgag gcccgtcagc
    18421 gagagcgcaa gcgcaggccc ctccaggctt agcccacgga gatattcgaa ctgccgggcc
    18481 gcgagaatcg ctcgccaggc gttgaacaca gccaatgata gcacgccgag cacgatcagg
    18541 gtaatccgaa agggacgctt cattgatcaa ggaaggagga ccaccttgcc gaattgctcg
    18601 ccgcgctcca ggatctcgtg ggcgacgcgt ccctcgctga gcggcagggt agtgtgaatc
    18661 accggcttca gcttgccctc gaagaccaat tgcatcacgc cgcgatagtc ctggtggctg
    18721 cccatcgtgc tgccgataat cgagatttgc ttggaccaca cgtaacgcac gtcggtgacc
    18781 gcctcccagc cgccggtatt ccccacggtc accaaccgcc cgccacgcgc cagcgcgcga
    18841 atgctctgca tccacgtcgc ctgcccgacg ttgtcgacga cgacgtcgac gccacgcttc
    18901 ccggtcgcca cgaacacggc cttcgaccag tcttcggcct tgtagttgat ggcgtaatcg
    18961 gcgcccagcg cccgggcctt ctccatcttt tcctcactcg acgtgatggc gtacaccgtc
    19021 gcgccgaggt gcttggcgac ctggacggcg agatgattga cgccgccccc cgcgccgacg
    19081 acggcgaccg tctctccaga tttcaatcgc gcccggtgca cgagcatgcg ccaggcggtc
    19141 agcccggcga gcgacgcggc cgcggctgct tcgtgtgaga cgtgatcggg catcggaagc
    19201 agattgctcg ccggcacgac gacgtattca gccgcgccgc cggtcgagtg ctcgcccagg
    19261 ataacgtaac cgggcgagag gctgtcctca ccccggcgcg tccactcgtc ttctttggtg
    19321 ctcaaacccg gatcgatggc gacgcgcgcg ccggccggcg gtccgcctgc accctcgccg
    19381 tgggaatcga cgatgccgct cacgtcacag ccgccgatgt gcggcttggg cagacgctcg
    19441 aaggtcggcc cgccgcgccg cacccacagg tcgagatggt tcagcgcgac ggccttcacg
    19501 cgcacccgaa cctggccggg tccaggctct ggatcgggca ggtcgccgta ttgcatcact
    19561 tcaggtccgc cgtgttcgtg gaagtacagt gctttcatgc aaggtcctcg taatccgtgt
    19621 catccgtggt tagtatagca ccaggccacg cggccggctg ctgcttcagg cgctcgatgc
    19681 ggcgctgcag gtatggcgag aagtacccat cgtagcgctc ccacagctcc ggaatgcgcc
    19741 agtcgtcgcc cgtcacgtgc cggcggcagt ggggggtgct gcactggcag tcgaactcgt
    19801 cgtacggcgt gccgtcgcac atggcatggt cgatgcacac ctcttcgccc ggggcaatat
    19861 cgcgcatggc gacgacggcg atctggccgc tcatgccagc gttggggtcg caactgtggt
    19921 tgatgtagtc ggccggctcg tccgggtaga gcgaggccag atagaggttc tcttccacct
    19981 ggacggtgtg ccgctgaatg tcaggcggca agtgctccca ttgctccaat gtgacgatct
    20041 ggccgctcca cacggccagc agctcgcccg ccgcgatcgg ctcgcgtgtg aatacgccat
    20101 agccgccctt atcggcgtgc tccctcactt cgaccttagg cgaaaggtac gacgcgaccc
    20161 gggtgggcac tggctcacct ccttgactgg agaggatgcg cggagcgccg cgaaaagatt
    20221 cacgccgagc cgtcatcgtg tggcgatgcc gcggctcggc gtgagacccc cgaaattgcc
    20281 tgcaaggcgc attctgcttc ggcctcgcta cttcaagccg agcacgtgac gcgcgatgac
    20341 caggcgctgg atctcgctgg tgccctcgta aatctctgtg atcttcgcgt cgcggaagta
    20401 gcgctccagc ggcatttcct tgctgtaacc catgccaccg tgaatctgca ccgctttctc
    20461 ggtgacccac atggcggtct cggaggcgtg cagcttggcg atgctggctt cgagcgtata
    20521 gcgctggccg tcgtgcttgg atcgctcctt ggccagcgcg gcgttccaga tgagcaggcg
    20581 ggacgcttcg atgcgcgtct tcatgtcggc cagcatccac tggatgccct ggaactggcc
    20641 gatgggctgg ccgaaggctt cgcgcgtctg ggcatactgc acgctggctt cgaaagccgc
    20701 ctcggccacg ccgagcgcct gcgcggcgat gccgatgcgc ccggcgtcga gcacgtgcat
    20761 ggcgatcttg aagccttcgc cttcttcgcc gagtcgattt tcgaccgggc agcggtagtt
    20821 gtcgtacacg atctcgcacg tggccgaggc gcggatgccc agcttcggct cggtcttacc
    20881 tttgacgaaa cccggctgct tcgtatcgac gatgaaagcc gtaatgcccc tgtgcctcct
    20941 ctcaggagcg gtcatcgtga acaggacgag ggcgtcggcg acagggccgg acgtcaccca
    21001 cgatttgcga ccgttgatga cgtagtgatc gccgtcgcgc actgcgcggc tcctcatgtt
    21061 gcccgcgtcc gacccgctca tcggctcggt cagcgagtag gcgccgaggg ccttgcccga
    21121 cgcgaccggc acgaggtact tgcgcttttg ctcttccgtg ccgaatttca ggatgccgtg
    21181 gcagtacagc gagttgttca ccgacatgat cgtgctgtgg ctcacgtcgg ccttggcgat
    21241 ctcctccatg gccagcacgt aggccagcgt gtcgaggccg gcgccgccgt attcttcagg
    21301 cacttccacg cccatcagtc ccagctcgcc catcttcatg actgtttcca gcggaaattc
    21361 gccagtctcg tcgaaatgtg gggcgatggg cgcgatctct ttctgggcga attcgcgcgc
    21421 cgcatcgcgg atcattttgt gctcttcggt cagctcgaga ttggggccgg cgtgtgtcag
    21481 ctctgcgata gccatgtgtc gctcctcgcg tgggtgattc tggagagtgc attgtgatta
    21541 tatcatagcg cgccccgccg tgaccatcgg tgctataatc gcgaggtaat tgtctaccgc
    21601 gtctactctc tgatacagac aatctgatca tgaagactgc cgcgtttcgc tttcacacgc
    21661 gccgttctgt ctcgatggcg atgaatggca tggtcgccac cagccagccg ctcgccgcga
    21721 tagccggcct gcgcatgctg ctgcggggcg gcaacgccgt cgacgccgcc gtcgcgacgg
    21781 ccgcggcgct gaacgtcgtc gagccgacgt cgaccggcgc gggcggcgac gtgttcgctt
    21841 tgacatacgt agcccggacc ggcgaggtaa ccgcgctgaa tgccagcggc tgcgcgccga
    21901 aggcgcagac gctggacgag atgcgccggc gcggacacac ggcgatgccc acgtacggtg
    21961 gactggcggt caccgtgccg ggggcggtcg cgggttggca cgacctcctg gtgcggcacg
    22021 gaacgatgac cctggcggac gtgctgcaac cggcgatcga tctcgccggg caggggttcc
    22081 cggtgacgga ggtcattgcc gggcactggt cgcgcctgga acgccgcgcc gatcagttcc
    22141 cggcgctggc gaagacgtat ctgatcgacg gccacgctcc ccggccaggc gaagtcttca
    22201 agaatcctga actcgctcgc acgctgcggg cgattgcaga gggcggcccc gacgccttct
    22261 accgcggccc gattgccgag tcgattgtga agatcatcca agagaacggt ggcctgatga
    22321 cgctcgacga catggccgca cacgcgtcga cgtgggacga gccgatccag actgactatc
    22381 gcggcgtgac gatcgtggag tgcccgccga acgggcaggg catcatcgcg ctcgaagcgc
    22441 tcaacatact cgaaggcttc aacctggtgg cgatggggta cggcacgccg gcctactatc
    22501 atcacctgat cgaggcggcg aagctggctt tcgccgacgc cttcgcttac gtcggcgacc
    22561 cacgcatggc gcatgtgccg atcgagggcc tgatcgataa ggcatacgcc gccgggcgtc
    22621 gccagtcgat cgacccggcg cgcgcggcgt cgacgcctgt acctggcagg ctgcgggacg
    22681 gcacggtgta tctcagcgtg atcgacaagg agcgcaacgc ctgctcgttc atcaacagca
    22741 actacatggg ctttggctcg ggtctggtcg ccgagggcgt cgcgctgcaa aaccggggcg
    22801 cgctgttttc gctggaggag ggccatccga actgtaccac gccgggcaag cgaccgtatc
    22861 acacgattat ccctgcgctg gcgctgaagc gtggcaagct gtggatgtcg ttcggcgtga
    22921 tgggcggctt cgttcagccg caggggcacg tgcaggtggc gtgcaacacg atcgacttcg
    22981 gcatgaaccc gcagcaggcg ttggacgcgc cgcgtgtcgt atggcacgac ggcgcgctga
    23041 tcgacgtgga ggacggcatc ggcgaagaca tgcgctcggc gctggcgaag atgggtcatg
    23101 acctgatgcc gaaagacgat ccgcgccgcc actacggcgg cgggcaaatc gtcgcgatcg
    23161 acgaggacag cggggcgctg ctgggcggca cggagccgag gaaagacggc gtggcgattg
    23221 gatactgacc agtgtttcag tgatcagtga gaccagtgat cagtgatcag tgatcagtgg
    23281 tcactgacaa ttgacaactg acaactgatt gctgattact gattactgat aactgatgac
    23341 tgacaactgc tcactccgcc gccgatgata tgctgagcaa ccttcctccc atctggccgg
    23401 tatccgaggt ggtcatcttc gactgcgaca gcacgctgag cggcgtcgag ggcatcgacg
    23461 agctggcgcg catggcgggc aggcaggaag acgtgtcggc gctgacacgc cgggcgatga
    23521 gcggcgacgt gccgctggaa agtgtgtacg agcatcgcct cgccgtgtcg aatcccaccc
    23581 aggcgcaggt gagccacctg cggcgtgtct atcgggagac cgtgattgcc gatgcgcgcg
    23641 aagttgtcgc ggccttgcag gacctcggct gccaggtctt cgtcgtcagc ggcggcctga
    23701 tcgagccggt gcgcgacttc ggcgcatggc tcggcgttcc acgcgatcac atttacgctg
    23761 tggggatgga gtacgaccag cttgcgggcg agtggtggcg gtattgggag cagcccggcg
    23821 ggcagaatcc caacgcgaat tacctggcgg tcaagagcag cccgctcgtc agcacaggcg
    23881 gcaagcgcgt ggtgatcgat cgtatccggc aacggtttcc aggccgggcg atgctcgtgg
    23941 gcgacggcac gtcagacctc gaagcccgaa cacaggtcga tctcttcgtc ggctttggcg
    24001 gtgtggttca tcgggaacgg gtggcggccc aagcgcccat atacgtcag
//
LOCUS       DBNHXB010000382         5354 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1954032, whole genome shotgun sequence.
ACCESSION   DBNHXB010000382 DBNHXB010000000
VERSION     DBNHXB010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1954032"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..899
                     /locus_tag="AC162G_15445"
     CDS             147..899
                     /locus_tag="AC162G_15445"
                     /inference="COORDINATES: protein motif:HMM:NF049367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5077249.1"
                     /translation="MSRLVMLGTAAAVPNASHQNTYMVLDGPGGAVLIDCAGSPIGRL
                     EQAGVPFAALNDVVITHIHPDHAYGFPLLLMGLWLLGRKAPLRVYALDGVLARLRAVM
                     DGYGWNDWPRFYPVLFEAVEEEVGAPVLETPDFRITAARNQHMTPTIGVRIEHKANGR
                     VAAYSSDTAPCDEIVELARRADLLFHEVAGETPGHSSAAMAGETARKAGVKKLVLVHY
                     QVSADPDDLAAEAEAAFGGPVEVAQDLAEYAL"
     gene            complement(956..1459)
                     /locus_tag="AC162G_15450"
     CDS             complement(956..1459)
                     /locus_tag="AC162G_15450"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR5077250.1"
                     /translation="MDTRYHPELIDPSAFVAPDARIVGQVTVGPESSVWFGSVLRGDT
                     DRVVIGTRTNLQDGCIVHVDAGSPAIIGSGVTVAHRAVVHGCTVEDDVLIGMGAILLS
                     GARIGRESIVGAGALVTGGTVVPARSLVLGLPGRVVRRVTDEELLSVRDTARRYAEYA
                     RRYRTGE"
     gene            1708..2139
                     /locus_tag="AC162G_15455"
     CDS             1708..2139
                     /locus_tag="AC162G_15455"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR5077251.1"
                     /translation="MGHGDRDDPHDRRRVRFDQLFTEMVGPVRWTVAQHTHAWRPPTD
                     VFETDDAVIIRVEVAGMRESDFNVTLADQLLIVTGYRQDPSPKVTYHQMELRYGDFRV
                     EVYLHWAIGESGIQAVYDNGFLQIVLPKARKQQIRIVETGT"
     gene            2262..4757
                     /gene="lon"
                     /locus_tag="AC162G_15460"
     CDS             2262..4757
                     /gene="lon"
                     /locus_tag="AC162G_15460"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR5077252.1"
                     /translation="MLAQHEESGAEQDADQIQIPDELPILPLRGVVVYPVTAVPLTVG
                     QPRSVHLIDEAVAEKKLVGLVASKDPSIEEPTPDDCYTVGTLASVQRLARMPDGTIRL
                     LVMGLERIRIIEYTSVQPYLKARVKRAPETVDEGLELEALRRKVIELFGRLVTLIPGI
                     PEEVGSMASAIEDARQLAYAIGTYIRIDLPDSQAILDFDAVSDKLRKIVTLLTQELEV
                     LELGHKIQSEAQSEMGKVQREYFLREQLKAIQRELGELDEQQMEVEEFRKKVAEAGMP
                     EEAHKEAVRELDRMAKLPTAAAEYGVIRTYLDWLVNVPWSKITPDNLDIGHARAVLDD
                     DHYGLSDIKERILEFLAVRKLRLERADEMPAADARVDYVRREREGVILCFVGPPGVGK
                     TSLGQSIARAIGRKFIRQSLGGVRDEAEIRGFRRTYIGSMPGRIIQALRRIETRNPVF
                     MLDEIDKLGMDFRGDPASALLEVLDPEQNREFRDHYLDVPFDLSQVMFICTANQIEPI
                     PGPLRDRMEIIQLSSYIDREKVRIAQGYLIPRQIKENGLRPGEVSFTDAAIQRVIADY
                     TREAGVRSLEREIGRACRKIVTRIAEGKAESVRIDAGDVPDLLGKKRFYGEIAERTQV
                     PGVVTGLVWTPVGGDLVFIEATSYPGGKGFVLTGQLGDVMRESAQAALSYVRTNARSL
                     DIPDDYFAKRDIHLHVPAGAQPKDGPSAGVTMATAIASLATGRPVRDDVAMTGEITLR
                     GQVLPIGGVKEKVLAAHRAGLKTIILPKRNENDIDDVPEEARKELHFILADRVEQVIE
                     AALREPGETHADGRRRSRRSIARKAPLEARRRS"
     gene            4754..5107
                     /locus_tag="AC162G_15465"
     CDS             4754..5107
                     /locus_tag="AC162G_15465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5077253.1"
                     /translation="MTKILIVDDDKATATLLRTLFEIEGLEGAVCSSAEQVLADVRSE
                     HPDLLLMDAHLAEVDSLGILRDLKADTVIGRIPVVVVSGMDRSDEFLPAGAAAFVLKP
                     FKPADLMSVIRRYLP"
     gene            5167..>5354
                     /locus_tag="AC162G_15470"
     CDS             5167..>5354
                     /locus_tag="AC162G_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR5077254.1"
                     /translation="MSSRRKRQTRRQWRRVDLHLHTPASSDYQVTGVSNLDILRAAAA
                     RSLDIIAFTDHNSIAGYAR"
BASE COUNT          996 a         1670 c         1754 g          934 t
ORIGIN      
        1 cttggcgaaa gctggctgct ggctctggcc gactacctga aagtcctcat ctttgtggcg
       61 ataccgtttc taattgtggc gtctctcatc gagatttacg tcacgcccgc cttattgtgc
      121 gcagcattgg gcggctgagg gggatgatgt ctcgactcgt catgctgggg actgcagccg
      181 cagtccccaa cgcgtctcac cagaacacgt acatggtgct ggacggcccc ggcggcgcgg
      241 tgctgatcga ctgcgcaggt agcccgatcg ggcggctcga gcaggcgggt gtgccgttcg
      301 cggcgctgaa tgacgtcgtc atcacgcata tccatcccga tcacgcctac ggcttcccgc
      361 tgctgctgat ggggctgtgg ctgctggggc gcaaggcgcc acttcgagtc tacgcgctgg
      421 acggcgtcct ggcgcgcctg cgggcggtga tggatggcta cggttggaat gactggccgc
      481 gcttctaccc ggtgctcttc gaggcggtcg aggaagaggt cggcgcgcct gttctggaga
      541 ctcccgactt tcggatcacc gccgcgcgca atcagcacat gacgccgaca atcggcgtgc
      601 gcatcgagca caaggcgaac ggtcgtgtag ccgcctattc gagtgacacg gcgccgtgtg
      661 atgagatcgt tgaattggcg cgcagagcgg atctgctgtt tcacgaagta gccggagaaa
      721 cgcccggcca ctcgagcgcc gcgatggcag gcgaaactgc tcgcaaagca ggcgtcaaaa
      781 agctagttct cgttcactat caggtcagcg ccgaccccga tgacctggcg gcggaggccg
      841 aggccgcatt tggcgggccg gtcgaagttg cgcaagacct ggctgaatac gcgttgtaag
      901 cagattcgtc cagacttccg aagtctccaa gacttcggaa gtctggcaag taaggtcact
      961 ctccggtacg atagcgcctg gcatactcgg cgtaacgccg cgccgtgtca cgcacggaca
     1021 gtagttcttc atccgtgacc cggcgcacga cccggccggg cagccccagc accagcgagc
     1081 gcgccgggac gactgtcccg ccggtgacca gcgcgcctgc cccgacgatg ctctcgcgcc
     1141 cgatgcgcgc gccgctgagc aggatggcgc ccataccgat cagcacgtcg tcctccaccg
     1201 tgcatccgtg aacgacagcc cgatgcgcaa cggtcacccc gctgccgatg atcgccgggg
     1261 atcccgcgtc gacgtgcacg atgcagccgt cttgtaggtt cgtgcgcgtg ccgatcacaa
     1321 cgcggtcagt atcgccgcgc agaactgagc cgaaccacac actcgactcg ggtccgaccg
     1381 tcacctgtcc cacgatccgg gcgtcaggcg cgacgaatgc gctcggatcg attagctcgg
     1441 ggtgataacg tgtgtccaac tctcctcctc aatcgccaat ccgaagatcc cggagtgcgg
     1501 ggctttagcc atgcagcctg aagcctgcac tccaattttc ggataggctc tgagcgcccg
     1561 gcaattgcgc tcgcaggcca tcctgtaagg caagaagcgg cgagaagtgg gggcaattct
     1621 accatgtgtt tgccatgagt atgcgatatg tggtacaatc gtttcggtca ggtgaagtga
     1681 acacagaaga catttcggag ggcggcaatg ggacacggtg atcgggatga tccgcacgac
     1741 cgcaggcggg tgcgattcga tcagttgttc acggaaatgg tcggcccggt gcgctggact
     1801 gtggcgcagc atactcatgc ctggcggcct ccaaccgacg tgttcgagac cgacgatgcc
     1861 gtcatcatcc gtgtcgaggt ggccggcatg cgcgaatcgg atttcaacgt gacgttagcc
     1921 gatcagttgc tgatcgtcac cggataccgg caggatccat cgccgaaggt gacttaccac
     1981 cagatggagc tgcgctacgg cgacttccgc gtcgaggtct atttgcactg ggcgatcggc
     2041 gagtccggca ttcaggccgt ttacgacaat ggattcctgc agatagtcct gccgaaagcg
     2101 cgcaagcagc agatccgcat cgtagaaacc ggaacctgag acccatcgca gcgtctgccc
     2161 attccccggt cccgagtcac gctaaccccg agtcgacaat gagcgcaact gaatttcctc
     2221 tggatggcag ccagcttttc cgccccgaag ctgtcggaac gatgctggct cagcacgaag
     2281 aatccggcgc cgagcaggac gccgatcaaa ttcagatccc ggacgagctg cccatcttgc
     2341 cgctgcgcgg cgtcgtcgtc tacccggtga cggccgtgcc gctgacagtc ggccagccgc
     2401 gctcggttca cctgatcgac gaggccgtcg ccgagaagaa gctggtcggc ctggtggcga
     2461 gcaaggatcc cagcatcgaa gagccgacgc cggacgattg ctacaccgtc ggcaccctcg
     2521 cttcggtgca gcggctggcg cgcatgcccg acggcaccat tcgcttgctg gtgatgggat
     2581 tggagcgcat tcggatcatc gagtacacga gcgtgcagcc ctacctgaag gcgcgcgtga
     2641 agcgcgctcc ggagaccgtc gacgaaggtc tcgagctgga ggcgctgcgc cgcaaggtga
     2701 tcgagctgtt cggccgcctg gtgacactca ttcccggcat ccccgaggag gtcgggtcga
     2761 tggcgtcggc gatcgaggac gcgcgccagt tggcgtatgc gatcggcacg tatattcgca
     2821 tcgatttgcc ggactcgcag gcgatcctcg acttcgatgc cgtctccgac aagctgcgca
     2881 agatcgtcac gctgctcacg caagagctgg aagtgctcga gctgggccac aagatccaga
     2941 gcgaggccca gtccgagatg ggcaaggtgc agcgcgaata ttttctgcgc gagcagctca
     3001 aggccatcca gcgcgagctg ggcgagctgg atgagcagca gatggaggtc gaggagtttc
     3061 gcaagaaggt cgccgaagcg ggcatgccgg aggaagccca taaggaagcg gtgcgcgagc
     3121 ttgaccgcat ggcgaagctg ccgacggcgg ccgccgagta cggcgtcatc cgcacgtatc
     3181 tcgactggct ggtcaacgtg ccctggtcga agatcacgcc ggacaacctc gatatcggcc
     3241 acgcgcgtgc cgtgctcgac gatgatcatt acggtctgag cgacatcaag gagcgcatcc
     3301 tggagtttct ggccgtgcgc aagctgcgcc tggagcgcgc cgacgaaatg ccggcggccg
     3361 acgcgcgcgt cgactacgtg cgccgcgagc gcgagggcgt gatcttgtgc ttcgtcgggc
     3421 cgcccggcgt gggcaagacg tcgctggggc aatcgatcgc gcgggcgatc gggcggaagt
     3481 tcatccgcca gtcgctgggc ggcgtgcgcg acgaggccga gattcgcggc ttccggcgca
     3541 cctacatcgg atcgatgccg ggccgcatca tccaggcgct gcgccgcatc gaaacgcgca
     3601 accccgtctt catgctcgac gagatcgaca agctgggaat ggatttcagg ggcgatccgg
     3661 cttccgcgct gctcgaagtg ctcgacccgg agcagaaccg cgagtttcgc gaccactacc
     3721 tggacgtgcc tttcgatctg tcgcaggtga tgttcatctg cacggcgaat cagatcgagc
     3781 cgatccccgg cccgctgcgc gaccgcatgg agatcattca gctctccagc tacatcgatc
     3841 gtgagaaggt tcggatcgcg caagggtatc tgataccccg gcagatcaag gagaacgggc
     3901 tgcggccggg agaagtctcg tttaccgatg cggccatcca gcgcgtgatc gccgactata
     3961 cgcgcgaggc cggcgtgcgc agcctggagc gcgagatcgg gcgcgcctgc cgcaagatcg
     4021 taacgcgcat cgccgagggc aaggcggaat ccgttcgcat cgacgccggc gacgtgccgg
     4081 atctcctcgg caagaagcgt ttctacggtg aaattgccga acgcacgcag gtgcccggag
     4141 tggtgaccgg gcttgtctgg acgccggtgg gcggtgatct cgtcttcatc gaggcgacgt
     4201 cctatcccgg cggcaaagga ttcgtgctga ccggccaact gggcgacgtg atgcgcgaga
     4261 gcgcgcaggc cgcgctgtcg tacgtgcgca cgaacgccag gtcgctcgac atcccggacg
     4321 actacttcgc caagcgtgac atccacctgc atgtgccggc cggcgcgcag cccaaagacg
     4381 gcccgtccgc gggcgtgacg atggcgacgg cgattgcctc cctggcgacc ggccggccgg
     4441 tgcgcgacga cgtggcgatg accggcgaga tcacgctgcg tgggcaggtg ctgcctatcg
     4501 gcggcgtgaa agagaaggtg ctggctgcgc atcgcgccgg gctgaagacg atcatcctgc
     4561 ccaagcgcaa cgaaaacgat atcgacgatg tgccggaaga ggcgcgcaag gagttgcact
     4621 tcattctggc cgaccgggtc gagcaagtca tcgaggctgc gctgcgcgag ccaggcgaaa
     4681 cgcacgcgga cggccggcgc aggtcgcggc gttcgatcgc gcgcaaggcg ccgcttgagg
     4741 cgcgacgacg atcatgacca agatcctgat tgtcgacgac gacaaagcga cggcgacgct
     4801 gctgcgcacc ctgttcgaga tcgaagggct ggaaggcgcc gtctgttcga gcgccgaaca
     4861 ggtgctcgcc gacgtccgta gcgagcatcc ggatttgctt ctgatggacg cgcacctagc
     4921 cgaagtcgac agtctgggca ttttgcgcga tctcaaagcc gacacggtga tcggcagaat
     4981 cccggtcgtc gtggtttcgg gcatggatcg cagcgacgag tttctgcccg ccggcgcagc
     5041 ggcgttcgtg cttaagcctt tcaagccggc cgatctgatg agcgtgatcc ggcgctatct
     5101 tccctgatct tacagccccg acgggtttca gaaaacccgt cggggctagg gcaagtgtga
     5161 cgacgcgtgt cttccagacg aaaacgccag acgcgacgcc agtggcggcg ggttgatctg
     5221 catctccata cgccggcttc gtccgattac caggtgaccg gcgtctcgaa cctcgacatc
     5281 ctgcgcgcag ccgcagcgcg cagcctcgac atcatcgcct tcaccgatca caattccata
     5341 gccggctacg cgcg
//
LOCUS       DBNHXB010000383         4329 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1618582, whole genome shotgun sequence.
ACCESSION   DBNHXB010000383 DBNHXB010000000
VERSION     DBNHXB010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1618582"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="AC162G_15475"
     CDS             complement(<1..227)
                     /locus_tag="AC162G_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077255.1"
                     /translation="MFNYLARLTIALALVMTPATPPVAGMSLARGVAGQGYRATATTP
                     PSASLPRVPSLQRYETPGGRRLMLPPPHIVEG"
     gene            complement(248..1141)
                     /locus_tag="AC162G_15480"
     CDS             complement(248..1141)
                     /locus_tag="AC162G_15480"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HIR5077256.1"
                     /translation="MGAYARWVLALGMLAVWVSGCQPTPSVSENGVVPTTLAEGQAFW
                     LRAGSQESMPMPISLDIGWGDAIWTPPDQEAVLQLADGSHLRLGADSHISVRAPYSFD
                     TGATFRLTQGRLDVDARGGRFLVESYREVPHSLRLVLINIRLSPVGTPSLFNLVFDGD
                     AVTTSVFAGTVDVRSGDVRGTLGAGWEAVLAVDQPLRIISPATSTPTPSNTPTPSRTA
                     TRTPTPTATPTATPTATVRIITPTATPSPTEQPEDSGPTLPPPTPVQPTDEPKPPTDE
                     PKPPTEEPPPPTPETRPTPSP"
     gene            complement(1157..1915)
                     /locus_tag="AC162G_15485"
     CDS             complement(1157..1915)
                     /locus_tag="AC162G_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5077257.1"
                     /translation="MEAIILVVDDSIEFTEMVKTFLEDHQYRVIAASTGLEALSYLTG
                     NHRVDAILLDVMMPGLDGWEACRRMREFTNVPILLLTALARETDIVHGLDLGADDYLV
                     KPFHLSELKARLEAHLRRARMAGWESPYPAFSDGELYVDLPNREVRLHGRHISLSPTE
                     FRLLACLVRNAGIVVTHKSLLSEVWGPDYVRDVRSLKLYIRYLRQKIEPDPGRPRYIL
                     TEWGIGYRFREITPPPRRPEPEVWSRDTPSPAKP"
     gene            complement(2191..2880)
                     /locus_tag="AC162G_15490"
     CDS             complement(2191..2880)
                     /locus_tag="AC162G_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5077258.1"
                     /translation="MNEVILVIDDDVEFVQYLKLRLEGFGYRVVTASDGPAGLELAAD
                     VNPDLITLDIMMPGMDGWEICRRLHAVSNAPILMITAKGRVGDVVRGLELGADDYLVK
                     PFNTSELLARVKAILRRARSPSTPQRTSVYAVRDIELDTDRRIVKVRGKRIDLTPTEF
                     KLLTCFMANAGRVLPHRYCLVEVWGPEYSDQVDYLKLYVRYLRQKIEEDPSKPEYILT
                     EWGVGYRMVDE"
     gene            2955..3524
                     /locus_tag="AC162G_15495"
     CDS             2955..3524
                     /locus_tag="AC162G_15495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5077259.1"
                     /translation="MWDMLYWLKRTPWDTGITPPELIDLVKRRFPRGGRAVDIGCGTG
                     TNAVFLAGHGFAVLGIDVSRRAIALARRRAGEAGVSCDFRVGDVSRLDRLPDAGLFDL
                     AIDIGCFHGLSSGARRRYARGLADRMARGGIYLLYAFCPRQRGWRRIGASPDDVGSLF
                     ADGFLVEDVKIGVDTSSGLASAWYTLRRT"
     gene            3603..4136
                     /locus_tag="AC162G_15500"
     CDS             3603..4136
                     /locus_tag="AC162G_15500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR5077260.1"
                     /translation="MIVVIPQSGKRMSTEQELFKKVMRRWASGVTIVTTRIGEDVYGL
                     TVSGFAGISLEPPLVMVSIGHNQSSHNWVREGGCYAVNFLRADQADLSDRFATVDVRH
                     RFAGLRYRTVASGAPVLDDCLAWFDCRLVAEHVVGDHTIFVGEVLIGEVLSDAPPLVY
                     FNRGYLPPSSFIPSSMR"
     gene            complement(4114..>4329)
                     /locus_tag="AC162G_15505"
     CDS             complement(4114..>4329)
                     /locus_tag="AC162G_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR5077261.1"
                     /translation="DPAFAPGTGTPEVGGFTSYDALQLVRGLQGLNLVGFDLVEVSPP
                     YDHGDITAILASNIAFEFLSLIALKKG"
BASE COUNT          778 a         1324 c         1427 g          800 t
ORIGIN      
        1 ccttcgacga tgtgcggcgg cggcagcatg agacggcggc cgccgggcgt ctcgtatctc
       61 tgaaggctgg gcacgcgcgg cagtgacgca ctcggtggtg tcgtcgccgt tgcccggtag
      121 ccctgacccg cgaccccgcg tgcgagcgac attccagcca cgggcggcgt ggccggcgtc
      181 atgacgagcg cgagagcgat agttaatcgg gcgagatagt tgaacacagg tctcaaggcg
      241 gcgacgtcta cggcgagggc gtcggcctgg tctccggcgt cggcggcggc ggctcctccg
      301 tcggcggctt cggttcatcc gtgggcggct tcggttcatc cgtcggctgc accggagtcg
      361 gaggaggcag agtcgggccg ctgtcttcgg gctgctcggt cggcgacggc gtagccgtcg
      421 gcgtgataat ccgcactgtc gccgttggag tcgcagtggg cgtcgccgtc ggcgtggggg
      481 ttcgagtcgc cgtccggctt ggcgtcggcg tgttcgaagg cgtgggcgtc gaggtcgcgg
      541 gcgagataat gcgcagcggc tgatcgacgg cgagcacagc ctcccacccc gctccgagcg
      601 tgccgcgcac gtctcccgac cgcacgtcga cggtgcccgc gaaaaccgaa gtggtcacag
      661 cgtccccatc gaacaccagg ttgaaaaggc tcggcgtgcc gacgggggac agtcgtatgt
      721 tgatcagcac gagacgcagc gagtgaggca cttcgcgata cgactccacg agaaagcggc
      781 cgccccgggc gtcgacgtca aggcgccctt gcgtgagacg gaacgtcgcg ccggtgtcaa
      841 acgagtaggg cgctcgcacg cttatgtggc tgtctgcacc cagacgcaag tggctgccgt
      901 ccgcgagctg caacaccgcc tcctgatcgg gaggggtcca tatggcgtca ccccaaccga
      961 tgtcgaggga gatcggcatc ggcatcgatt cctggctgcc ggcgcgcagc cagaacgcct
     1021 gcccctcagc cagggtcgtc ggcacgacgc cattctctga cacgcttgga gttggctggc
     1081 agccagaaac ccacacggct agcatgccca gcgccaacac ccatctggcg tatgcaccca
     1141 tcgttttaca cggagttcat ggcttggccg gtgacggcgt atcacgcgac cacacttccg
     1201 gctcgggcct gcgcggcggt ggcgtgattt cacgaaagcg atagccgata ccccattcgg
     1261 tgagaatata gcgtgggcgg ccggggtccg gctcgatctt ctggcgcaga tagcgaatgt
     1321 acaatttgag tgaccggaca tcccgcacgt aatccggccc ccatacttcg gacagcaaac
     1381 tcttgtgagt gacgacgatg ccggcgttgc gcacgagaca ggccagcagc cgaaactcgg
     1441 tgggtgacag ggagatatgc cggccgtgca ggcgcacttc acgattgggc aagtcgacgt
     1501 acagctcgcc gtcggagaac gccgggtaag ggctttccca gccggccatc cgggcgcggc
     1561 gcaggtgcgc ctccaggcgc gccttaagct cgctcaagtg aaagggcttt acaagatagt
     1621 cgtccgcgcc caggtcgagg ccgtgcacga tgtctgtctc acgagccagc gccgtcaaca
     1681 atagaatagg aacgttggtg aattcgcgca tacgccggca ggcttcccag ccgtccaagc
     1741 cgggcatcat cacgtcaagc aggatagcat ccacgcgatg gttgccggtg agataactga
     1801 gagcttcaag gcccgtcgag gccgcgatca ctctgtactg gtggtcctcc aggaaagtct
     1861 tgaccatttc cgtgaactcg atgctgtcgt cgaccaccag gatgatggct tccatgcttg
     1921 gcgtgctcct gacgggccat ctgcaagtgc cggagtggct cagccgtgcc gaagcacgac
     1981 accgacttga gcatgcccgt cagattacaa actaataggg catagttaga tcgaggggag
     2041 agatctgtaa gaaaagtgta tgccctaatg gaaagggaga gcctgggaac tctccctccc
     2101 gaaaaacagg caaaatcgta aggagcgcac acgccggtca tgtctgtttc gccacccggt
     2161 ccacttcggg tgagatgggt cagacgcgca ctattcgtca accatccggt agccgacacc
     2221 ccattccgtg agtatgtatt cgggcttaga tggatcctcc tcgatcttct ggcgcaggta
     2281 tcgcacgtag agcttgaggt agtccacctg gtcgctgtac tccggtcccc acacctcgac
     2341 caggcagtag cgatgcggta ggacgcgccc ggcattcgcc atgaagcaag tcagcagctt
     2401 gaactcggtt ggtgtgaggt ctatgcgctt gccacggact ttgacgatgc gccggtcggt
     2461 gtccagctcg atgtcgcgta ccgcgtagac actcgtgcgt tggggcgtgc tgggggacct
     2521 ggcgcggcgc aaaatggctt tcacccgggc gagcaactcg ctcgtgttga agggttttac
     2581 tagatagtcg tccgcgccta gctcgaggcc gcgcaccaca tcgccgacgc gacccttggc
     2641 ggtgatcatc aggatgggcg cgttcgacac ggcatgcagc cgccggcaga tttcccagcc
     2701 gtccatgccg ggcatcatga tatcgagtgt aatcagatcg ggattgacgt ccgcggctag
     2761 ctcaagcccg gctggcccgt cggaggcggt gaccacccgg tagccgaagc cctccaggcg
     2821 cagcttgaga tattgcacga attcaacatc gtcgtcgata acgagaataa cttcgttcat
     2881 ggtcctctct cggaaggctc tacaagcgga gattatatca caactcattt ggcaagagag
     2941 gtgtgcaacc tatgatgtgg gacatgctct actggctcaa gcggacgccg tgggatacgg
     3001 gtatcacccc tcccgagctg atcgatttgg tgaagcggcg cttcccgcgc ggcgggcgcg
     3061 ccgtcgacat cgggtgcggg acgggcacga acgccgtatt cctggccggg cacggcttcg
     3121 cggtgctcgg cattgacgtg tcccgccgag cgattgcgct ggcccgccgg cgggccggcg
     3181 aagccggcgt gtcctgtgac tttcgtgtgg gcgatgtgag tcgcctggat cgactgccgg
     3241 acgcgggttt attcgatctg gcgatcgata tcggatgctt ccacggattg tcttccgggg
     3301 cacgacgacg atacgcccgg gggcttgccg atcgcatggc gcgcggcggc atttacctgc
     3361 tctatgcgtt ctgcccgcgc caacgggggt ggcgccgtat cggcgcgtcg ccggacgacg
     3421 tcggctcgct gttcgcagac ggcttcctgg tcgaggacgt gaagatcggc gtggacacaa
     3481 gcagcggcct cgcctcggcg tggtacacgc ttcggcgcac atgacgagac gcgtggagcg
     3541 cctgacccgg ccgaagggac aggctggccg cctgaagggt tgacggtcgc ctgcaggctg
     3601 ctatgatcgt cgtcatcccg cagtcaggca agcgcatgtc gacagaacaa gagctattta
     3661 agaaagtgat gcgccgctgg gcgagcggcg tgacaatcgt gacgacgcga atcggggagg
     3721 acgtctacgg gctgacggtg tccggattcg ccggcatctc gctcgagcca cccctcgtca
     3781 tggtgtcgat cggccacaac cagagcagcc acaactgggt tcgcgagggc ggctgctacg
     3841 ccgtcaattt cctgcgcgcg gatcaggccg acctgtccga tcgctttgcc acagtcgatg
     3901 tccggcatcg cttcgcaggc ttgcgttatc gcacggttgc cagcggcgcg cctgttctcg
     3961 acgactgcct ggcatggttc gactgccgcc tcgtggccga gcacgtcgtc ggcgatcaca
     4021 cgatattcgt tggcgaggtg ctgatcggcg aggtgctgag cgacgcaccg ccgctggtgt
     4081 atttcaatcg aggctatctt ccgccttcat ccttcatccc ttcttcaatg cgatgagcga
     4141 caggaactcg aaggcgatgt tcgaggcgag gatcgccgtg atgtcgccgt gatcgtaggg
     4201 cggcgagact tcgacgagat cgaatcccac caggttcagc ccctgcaagc cgcgcacgag
     4261 ctgcagggcg tcgtacgacg tgaacccgcc gacctccggc gtgccggtcc ccggcgcgaa
     4321 agccggatc
//
LOCUS       DBNHXB010000384         4668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1785751, whole genome shotgun sequence.
ACCESSION   DBNHXB010000384 DBNHXB010000000
VERSION     DBNHXB010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1785751"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="AC162G_15510"
     CDS             complement(<1..380)
                     /locus_tag="AC162G_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010491792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR5077262.1"
                     /translation="MNEALHRKLTLICAPAGFGKTTLVSEWVTGCGRPAAWLSLDEGD
                     SDSTRFLTYLVAALQMIAANIGEGVLGALQSPQPPPTESILTALLSQTTTIPDNFVLV
                     LDDYHVIDSKPVDNALTFLLEHLPP"
     gene            complement(661..1926)
                     /locus_tag="AC162G_15515"
     CDS             complement(661..1926)
                     /locus_tag="AC162G_15515"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5077263.1"
                     /translation="MPSASQVSQSIIRRIMATLFVAQGMALAALVASTTVSSIIGAEL
                     TGSERGAGLPSTLTLIGGALAAYLAGRLMNRVGRRIGLSAGYLCGLAGGLLAGIGVID
                     RSLVIFLIGMILLGAARAISDLSRYAAADVYTARMRGRAVSTIVFAGTIGAILGPILA
                     PVAGQMSESLGYSELAGPWFATAAIFVFALVFINVFLHPDPRDIARAMHREGDFGQPQ
                     SDGQAARSFRQVMSAPAARLALISLGLAQTVMVTVMTITPVHMTHYNHGLDAIGLVVS
                     AHIVGMYGLSFVTGWLSDRWGRRATIGLGSLLLIAACVLAPQDSNTLPLAASLFLLGF
                     GWNMCFVAGSSLLTDTLAVHERARIQGAADLVVNLASAAGSLASGILIASLGFGTLAL
                     ISASLALIVIAATLKTQQLQPVKMMEQAP"
     gene            complement(2021..3400)
                     /locus_tag="AC162G_15520"
     CDS             complement(2021..3400)
                     /locus_tag="AC162G_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR5077264.1"
                     /translation="MDDRIIDFIAALRAMGVRISLAESQDAMRAVEHLGVMNREAFRI
                     ALKTTLVKESADGPIFDRLFPLFFSSEVPPMMDGLGGLSPEEQEMLREALRQMAREIA
                     RRMRQLMEGRGLSHEELEQLGQQAGLPNARSPYQQPWITRRMLQQLGFNSLEEALEQL
                     MQMLAAMGMGQESMDQLSEAVGQNADAMREQVERFAGAGIARNMAEEYQRRPPIHDLM
                     NRPLQSLSEEEAEELREQVRRLAAHLRSRAALRQKRGKKGQLDAKATLRASLKYGSVP
                     MEIKHKHRHLKPKLCLICDVSTSMRPVVEFMLRLMYELNDQIAKARSFAFIDDITEIS
                     HDFAENPAEVAVYQVMERLHPGYYNTDLGYSLAHFVKDYLDAIDRRTTVIVLGDGRNN
                     FNDPRLDALDAIKRRARKVLWFNPEFRGQWGTGDSDMLLYAPLCDAVHTVSTLAQLAD
                     AVDHLFDAH"
     gene            complement(3544..4509)
                     /locus_tag="AC162G_15525"
     CDS             complement(3544..4509)
                     /locus_tag="AC162G_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5077265.1"
                     /translation="MPTDTAQLKDALKTQQYIASDEITTVLFLADHLNKPVLAEGPAG
                     VGKTELGKAWAAATCRPLIRLQCYEGLDETKALYEWEYAKQMLYTQLLRDKLADLMTD
                     TRTLGEAADRLAAEEDVFFSDRFLLARPLLKAIISDAPVLLLIDEIDRADAEFEAFLL
                     EVLSDFQVSVPELGTLKARHQPLVVLTSNNTRELSEALKRRCLYLFIDYPTVDAELNI
                     VRLKVPNLAPQLARQAVEFVQRLRKLDLKKAPSISETLDWAKALVALNARSIDRKTLD
                     TTITVLLKHESDVMRARRAAGQSATDYSEDEDSSFRQLPRRARSN"
BASE COUNT          850 a         1371 c         1569 g          878 t
ORIGIN      
        1 ggtggcaggt gctcgagcag aaaggtgagg gcattgtcaa ccggtttgga atcaataacg
       61 tggtagtcgt caaggacgag gacgaaattg tccgggatgg tggttgtttg actgagcagg
      121 gctgtcagga tcgattcggt tggcggtggc tgaggagact ggagcgcgcc caacacccct
      181 tctccaatat tcgccgcaat catttgcaga gcagcgacaa ggtaagttag aaagcgtgtg
      241 gagtcgctat ccccttcgtc cagcgacagc caggcggcgg gcctcccgca accagtaacc
      301 cattcgctga ccaacgtggt tttaccaaag ccggcggggg cacagatgag ggtcagtttg
      361 cggtgcagag cctcgttcat ccgctcgatc aggcgggggc ggaggacaac tttaggccga
      421 ggcggaggga tataaagttt agtggctaaa attggcgtag acatagatca gttatgaaac
      481 acacacccca aaacaaccca cctcccaaaa actgctagac caactgcact acacaagtat
      541 actgcgaaaa cgggtgacat tcgaaagcac ggcctgcccc ggcacacaaa gacttgggaa
      601 gtctcgacgg gggctctgaa gtccgaaata ccgtgagggg gggggaagct caccttcttc
      661 tcagggtgcc tgctccatca tcttgactgg ctgtagctgc tgcgtcttga gtgtggctgc
      721 gatgacgatc aatgccaggc tcgcgctgat gagagccaga gtcccgaatc ccagggaggc
      781 gatcaggatg ccgctggcca gactgccggc tgccgaagcc aggttgacga cgagatcggc
      841 cgcgccctga atgcgtgcgc gctcgtgaac ggcgagtgtg tcggtcaaca gtgacgaccc
      901 ggcgacgaag cacatgttcc agccgaagcc gagcaggaag agcgaggcgg ccagcggcag
      961 cgtattcgag tcttgcggcg caagcacgca cgcggcgatg aggagcaggc tgccgaggcc
     1021 gatcgtggcg cggcgtcccc agcggtcgct caaccaaccg gtcacgaatg aaagcccgta
     1081 cattcccacg atgtgcgccg atacgaccag cccgatcgcg tcgaggccgt ggttgtagtg
     1141 tgtcatgtgg acgggcgtga tcgtcatcac cgtgaccatg acggtctgcg ccaggccgag
     1201 actgatcaag gctagccgtg ccgccggtgc ggacatcacc tgccggaacg atcgcgctgc
     1261 ctggccgtcc gattggggct gcccgaaatc gccctcccga tgcatcgcgc gcgcgatgtc
     1321 gcgcgggtcg ggatgcagga agacgttgat gaagaccagg gcgaagacaa agatggccgc
     1381 ggtcgcgaac catgggccgg ccagctcgct atagcccagg ctttccgaca tttggcccgc
     1441 aaccggcgcc agaatcggcc ccaagatcgc gccgatcgtt ccggcaaaga cgatcgtgct
     1501 gacggcgcgc ccgcgcatcc gagccgtgta gacgtcggcg gcggcgtagc gcgacagatc
     1561 ggagatcgct cgcgctgcgc cgagcaggat catgccgatc aggaagatga caagcgagcg
     1621 gtcgatgacc ccaatgcccg cgagcagccc gccggcgagt ccgcacaggt agcccgcgct
     1681 caacccgatg cgccgtccca cccggttcat caggcgaccc gcaagatagg ccgccagcgc
     1741 gcccccgatc aacgtcaacg tcgacggcag gccggcgccc cgctcgctgc cggtcaattc
     1801 ggcgccgatg atgctcgaca ccgtcgtcga tgcgacgagc gcggccaggg ccatgccctg
     1861 ggcgacgaag agcgtggcca tgatgcggcg gatgatagat tgtgaaacct ggcttgcact
     1921 gggcattctg gcttgcctcc cggttcgagc gatgatacca acgagcgacg cactttagga
     1981 agtgcgtcgc tcgtcgcggc gtcttcgccg ttcagtgttc tcaatgcgcg tcaaacaagt
     2041 ggtcgaccgc gtccgccaat tgggccagcg tgctgacggt gtgcacggcg tcgcacagcg
     2101 gcgcgtacag cagcatgtcg ctgtcgccgg tgccccattg tccgcggaat tctggattga
     2161 accacaggac tttgcgggcg cgtcgtttga tcgcgtcgag cgcgtcgaga cgcggatcgt
     2221 tgaagttgtt gcgcccgtcg ccgaggacga tcacggtcgt gcggcgatcg atcgcgtcga
     2281 gatagtcttt cacgaagtgg gcgagtgagt agccgagatc ggtgttgtag tagccggggt
     2341 gcaggcgctc catcacctga tacacggcga cctcggctgg gttttcggcg aagtcgtggc
     2401 tgatctcggt gatgtcgtcg atgaaggcga acgagcgcgc tttcgcgatc tggtcgttca
     2461 gctcgtacat caggcgcagc atgaactcga cgaccggacg catcgaggtg gaaacgtcgc
     2521 agatcaggca cagcttgggc ttgaggtggc ggtgcttgtg cttgatctcc atgggcacgc
     2581 tgccgtactt gaggctggcg cgcagcgtcg ccttcgcgtc gagctggccc ttcttgccgc
     2641 gtttctggcg cagcgcggcg cggctgcgca ggtgagccgc caatcggcgc acctgttccc
     2701 gcagctcttc cgcttcctct tcgctcagcg attgcagcgg ccggttcatc aggtcgtgga
     2761 tcggcgggcg gcgctggtat tcttcggcca tgttacgggc gatgcccgcg ccggcgaacc
     2821 gctcgacctg ctcccgcatg gcgtcggcgt tctggccgac ggcttcggac agttgatcca
     2881 tcgactcctg gcccatgccc atcgccgcga gcatctgcat caactgctcc agcgcttctt
     2941 ccaggctgtt gaaccccaac tgctgaagca tgcgccgggt gatccagggc tgctggtacg
     3001 gcgagcgcgc gttgggcagg ccggcctgct ggccgagctg ctcaagctct tcgtgcgaca
     3061 gcccgcggcc ctccatgagc tgccgcatgc gccgggcgat ctcgcgcgcc atctgccgaa
     3121 gcgcctcgcg cagcatctcc tgctcttcgg gcgacagccc acccaggccg tccatcatcg
     3181 gcggcacttc cgagctgaaa aacagcggga acaggcggtc gaagatcggc ccgtcggccg
     3241 actccttgac gagcgtcgtc ttgagcgcga tgcggaatgc ctcgcgattc atcacgccca
     3301 ggtgctcgac ggcgcgcatc gcgtcctggc tttccgcgag cgaaatgcgg acgcccatcg
     3361 cgcgcagggc ggctatgaag tcgatgatgc ggtcgtccac ggagcgtacc tttggttatg
     3421 ggtgaacact gaccggtgat cagtgaccgg tgatcagtga ccagtgatca gtgaccagtg
     3481 atcagtggat gccacgactt gtcacggttc actgattact gttcactgat tactgattac
     3541 tgctcaattg cttctcgctc gtctcggcaa ttgccgaaaa gaggagtctt catcttccga
     3601 gtaatctgtt gcagactggc cggcggcgcg gcgggcgcgc atcacgtccg attcgtgctt
     3661 gagcaggacg gtgatcgtcg tgtcgagcgt cttccggtcg atcgatcggg cgttgagcgc
     3721 gacgagcgcc ttggcccagt cgagcgtctc gctgatcgag ggcgccttct ttaggtcgag
     3781 cttgcgcagg cgctggacga actcgacggc ctggcgggcg agctgcggcg cgaggttggg
     3841 cactttcagg cggacgatat tcagctcggc atcgacggtc ggatagtcga tgaacaggta
     3901 caagcagcgg cgcttgagcg cctcgctcag ctcacgcgtg ttgttgctcg tcaacacgac
     3961 gagcggctgg tgtctggcct tgagcgtccc cagctcgggc acgctcacct ggaagtcgct
     4021 cagcacttcg agcaggaagg cttcgaactc ggcgtcagcg cggtcgatct cgtcgatcag
     4081 aagcagcacg ggtgcgtcgc tgataatcgc cttgagcagc ggccgggcga gcaggaagcg
     4141 atcggagaag aaaacgtcct cctcggcggc gaggcggtcg gcagcttcgc cgagcgtgcg
     4201 ggtatcggtc atcagatcgg cgagcttgtc gcgcagcagt tgcgtgtaca gcatctgctt
     4261 ggcgtattcc cactcgtaca gcgccttggt ctcgtccagc ccttcgtagc actgcaggcg
     4321 aatcaggggc cggcacgtgg cggcggccca cgccttgccc agctcggtct tgccgacgcc
     4381 ggccgggcct tcggcgagca ccggcttgtt gagatgatcg gcgaggaaca agacggtcgt
     4441 gatttcgtcg gaagcgatgt attgctgggt tttgagagcg tccttgagct gggccgtgtc
     4501 ggtaggcatg cgagtccttt ccgggcgaat ggctgaattg taaccttgtt tggaggaaag
     4561 tggcaaggtg agtgaggggt gcggagagag ggggggtggg agttagagtg gggagttaga
     4621 gtggggagtt agagtgggga gttagagcta gagttggcgc ttcggttg
//
LOCUS       DBNHXB010000385        11569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2961238, whole genome shotgun sequence.
ACCESSION   DBNHXB010000385 DBNHXB010000000
VERSION     DBNHXB010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11569
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2961238"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="AC162G_15530"
     CDS             complement(<1..847)
                     /locus_tag="AC162G_15530"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR5077266.1"
                     /translation="MIEAALKPISLADGLTKLQLVSLDACTRCWACLDWCPVFDILHD
                     ETISPPEKIREYGNLVRAQENPVARLFGRPALDRPRLERLSLALYTCTTCGRCGEVCE
                     VGIHTQRLWPALRAKIVELGCGPIGPQRDVPRILAEKHNPYDKPHADRFAWVPDDIHV
                     AERAEVGFFAGCSGAYVAQPMMVGAVRMLQAAGIPFTLFGDEYCCGVPLWVLGYRDGL
                     AELVQHNVESFAARGVKRLVVSCPCCLDHLERRWPEFYGRPLPFEILHVTQVIAEEME
                     AGNLKL"
     gene            complement(844..1650)
                     /locus_tag="AC162G_15535"
     CDS             complement(844..1650)
                     /locus_tag="AC162G_15535"
                     /inference="COORDINATES: protein motif:HMM:NF014695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HIR5077267.1"
                     /translation="MHTLFQSLIVIPPAFRAIFNVAALAVLAISALASLGRMSIWLRG
                     EDKDSGPIAGRGLLGALWLTLFQALSPDCLFARRVFARSKVRGVMLMLISWSFVVLWL
                     GVLISLTEVILPQASIDRLQFLLAPVMDIAGGLLLVGLLMALARRYLFRPVKTISVAA
                     DGVVLWLFAVTVLLGFVLEGVRLSGSGWLLAQYWPVGALFGEILRVLAGGGSLYARWY
                     TAAYLAHAAAGFALIAYLPFSKLFHLFAAQITTAAARQRRVAVSGKEGVG"
     gene            complement(1735..3225)
                     /locus_tag="AC162G_15540"
     CDS             complement(1735..3225)
                     /locus_tag="AC162G_15540"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5077268.1"
                     /translation="MEQIRSALPTSFLKAITVGLLLTLVACQVTTPAPTATPAPTATP
                     KPTATPAPSPTPLPELTGDAVRGGLLYDNWWTVIGADTPTTDQPLWKTQTTNTRTGAD
                     TWRCKECHGWDYKGVEGAYGSGSHQTGFKGIYAARDKHPFEILTSLKGGTNPDHDFSA
                     VMDEQALTDLALFVAQAQMDAADLVSADKTPTGGNPETGKVVFEDTCVTCHGPQGTAI
                     NFGDTAEPEYVGTIASDNPWEFIHKVRFGQPGTEMPSGIAEGWEQEDFADLLAYAQTL
                     PTAPSTSLGGLLYDKWWAVTGADEPATDQPLWKTQTTNTRTGADTWRCKECHGWDYKG
                     VEGAYGSGSHKTGFKGILASASLSAEELAAWLTGTANPDHDFSAVLEEYQIDALAAFV
                     RDGLVDTAAYVNADKTVNGDPAEGKTRFDDTCTACHGEDGKTLNFGDEANPEYVGTIA
                     SDNPWEFFHKAWFGQPGTSMPAGIDLGWTLEDIANLLAYAQTLPAK"
     gene            complement(3243..5333)
                     /locus_tag="AC162G_15545"
     CDS             complement(3243..5333)
                     /locus_tag="AC162G_15545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmcA/MtrC family decaheme c-type cytochrome"
                     /protein_id="HIR5077269.1"
                     /translation="MKGHAIRLASLGLLGLGLALTLTACAGAPGEAGPPGPQGPSGPQ
                     GLAGPAGPQGQAFVVPGPGLNIEITSVEFPADGKPVVSLTLADADGRPQKPEALEGYG
                     FTVAQIVVDADTGLSNYQNLLVSEVEGQPFTVGGETQQPALAKATQPFAESGGAWQSV
                     GDGQYTYTFTNTLTSEVDPSLTTSVGLYAYKDGRASVANDVFTFVPAGGAPSVTREVV
                     ATQNCNNCHNPLAIHGGVRRETVLCVTCHTDQNIDPETGNVLNLRVFIHKIHRGEFLP
                     SVLAGQPYEIIGFRQSSHDYSHVAWPQDVRNCTTCHTGGADSDNYKTKPQIAVCTACH
                     DDVNLETGENHPGGKQADGTCANCHAPDGEEFDASITGAHTIPSNSTQIKGVNFEIVS
                     VEGAAPGGSPVVTFKVTDNSGAAIAPADMDYLAVNLAGPTSDYVTRVTETIFRAPSDT
                     PPAVEDAGAGAYSYTFDYTLPDDATGTYAVGMEGYVMETIEGVADPVRVPGFNPVTYV
                     ALDGGEPAPRRQVVDREKCNACHKDLALHGTIRKNTEYCVMCHNTTASDEEVRPAEAM
                     PPASIHFKVLIHKIHLGEERSQRPFIVYGFGGSTHDFTELLFPGNLADCETCHRPGTY
                     GLPLPRGSQPTIVTQAGNVVSTILPTRAVCTACHDGAAAGGHIDLQTTASGLETCEVC
                     HGAGKEFDVTTVHR"
     gene            complement(5350..5631)
                     /locus_tag="AC162G_15550"
     CDS             complement(5350..5631)
                     /locus_tag="AC162G_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077270.1"
                     /translation="MRLLLNRPDIEWTGATSDYAAAKDAILDLRPDTVLVEEVGGSVP
                     PEVMEILETIPWNMRMISLGLADNKLSVYHRERRTIEQPEDLLRLIQGE"
     gene            complement(5969..6625)
                     /locus_tag="AC162G_15555"
     CDS             complement(5969..6625)
                     /locus_tag="AC162G_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5077271.1"
                     /translation="MADKVQVLIADDHTIVRSGVRLLLQAEPDIEVVGEALNGDEAVA
                     LAESLRPDMVLMDIAMPGMDGLEATRQIKARFPDMHVLVLTMHRSDEYFFEMLKAGAS
                     GYVLKGAETNELIGAIRAVARGEVFLYPTMAKQLLQDYLNRLKEGAGLPQPTLTPRER
                     EILRLLAEGYSNKEIAERLIVSPSTIHSHRTNLMRKLDLSSRHDLIRYARERGLIRDF
                     "
     gene            complement(6618..8132)
                     /locus_tag="AC162G_15560"
     CDS             complement(6618..8132)
                     /locus_tag="AC162G_15560"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR5077272.1"
                     /translation="MSAGHVFPRFVRDSLRAKVTLGVVLPLILILGSFTAIQYARQRE
                     AVLANLSFLAAQTGQVIENSLQHAMLTQDLDGLQHVLDAIGEGETVRVIYLLDTSGRV
                     IFAAKGQGVGAQLDNREPNCQTCHQLPAAKRPGSVVITLSDGQRVFRSMNPIENRPAC
                     QACHDPGQRLNGLLLTDIPMAPLEAPLAASLQENLLWWGGTILTTVIVVNLAMSSLVI
                     RRLEAVAQALARFGRGQLGLRLPANNPDEIGQLAAAFNEMGRRIQSEEAENRALSEGL
                     RREAGHRYELLKRLIAAQEDERRRVARDLHDGLGQDLAGLALRLEAIEKQAVHQPEQA
                     RVQLQQARALITEATERAYVMILSLRPSALDDLGLAPALRAHAERALKDTGVHFELES
                     RDLTRRLPLEIETALFRIFQEALTNVTRHAGARKVCVSLAARDGSFEGEIADDGQGFD
                     LREVQANGHGPRGLGLRGMEERVAQCGGTLDILSQPGAGTRVRIRIPLPEADGG"
     gene            complement(8275..10377)
                     /locus_tag="AC162G_15565"
     CDS             complement(8275..10377)
                     /locus_tag="AC162G_15565"
                     /inference="COORDINATES: protein motif:HMM:NF012262.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5077273.1"
                     /translation="MNEQEKQEYLEQYKQAKEHGVPFFPDALIKDAVVALLVFVILVA
                     LATFVGAPLEARADPADTSYTPRPEWYFLFLFQLLKYFPGQLEVIGVVVIPTLVVILL
                     FLLPFLDRSPRRHFLGRRVATGVTLLAVVGITFLTVQALREAPPPVEVVEGDRTAALY
                     AANCAGCHGPSIDVPSGANLHDVIAQGKHEGMPAWSADLTTDQIDALAGFILSPGGSR
                     LFTQNCGACHEAPELVAGDPRELKSVLDQGPAYPAHADVEVPVWAEVLSHEDHIELLN
                     FLVAPDGQRLFATNCASCHGRAVAFSGEESELRGIISQGGLHLTMPPWRERLSDSELD
                     TLARYVVDPGSTPEGAGVFAQYCSSCHGERVPSAADVAQARETIASGGAHETMPVWGS
                     VLTPEQLDALVVYTLQAASGAPIALGQQLFAENCATCHGELGEGGPNPTRPDDVIAPI
                     SSAEYLKTRDDATLRSVVAQGQPNFGMSPFGTAFGGPLEDDEIEAIVAYFRSWEANPP
                     VELPPEVAATRLSLSGSEVYTDLCTQCHGANGEGGIGPSLRDPEFQAENTDQELFDTI
                     NLGHSATPMIAWGEVLTSEQIEQLVDFLRQLGSAEQGRPAGIPSFSADVLPIFHARCA
                     VCHGSLGGWDSSSYESVMSSGEHAPAVIAGDVDGSLLAQKIRGTQTQGTMMPPGGLLP
                     EDEIQVILDWIAAGAPDN"
     gene            complement(10381..11040)
                     /locus_tag="AC162G_15570"
     CDS             complement(10381..11040)
                     /locus_tag="AC162G_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR5077274.1"
                     /translation="MSTRLGNWLDERSGWRRAWEAIFLRQVPKVNWLYTLGSATLFVA
                     INQIVTGILLTIYYVPTPDQAYDSVQYITAEVSAGWLIRGLHHWGASAMVVLAVLHML
                     RVILNGSYKFPREMTWVTGVALLLVVLGFSFTGYLLPWDQKAYWATTVGTQIAGTPPV
                     IGDTILRIVRGGEELTAVTLARFFGVHVWMLPAVLMILIGIHLYLVVRIGISAVPKRG
                     E"
     gene            complement(11042..11521)
                     /locus_tag="AC162G_15575"
     CDS             complement(11042..11521)
                     /locus_tag="AC162G_15575"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR5077275.1"
                     /translation="MDEERKIGRRKFLSLTTWAIGGVISAGLAIPAIAYIVGPALKRE
                     EAQDWIRLGSASKVELGTPTLFKTKIKRRTGWIVNEEERTVYVLTENGRDFIAMSNIC
                     THLGCRVRWIAQQGQFFCPCHNGIFAKDGRVISGPPPRPLDRYEVKVEDDQLFILGG"
BASE COUNT         2081 a         3645 c         3678 g         2165 t
ORIGIN      
        1 tcagtttcag gttgccggct tccatctcct cggcgatgac ttgcgtgacg tggaggatct
       61 caaagggcag cgggcggccg tagaactccg gccagcggcg ctcgagatga tccaggcagc
      121 acgggcatga gacgaccaga cgtttcacac cccgggccgc aaagctttcg acattgtgct
      181 gcaccaactc ggccagacca tcgcgatagc ccagcaccca cagcgggacg ccgcagcaat
      241 attcgtctcc gaacagcgtg aaggggatgc cagccgcttg cagcatccgc acggcgccga
      301 ccatcatcgg ctgcgcgacg taggccccgc tgcacccggc aaagaatccc acctcggcgc
      361 gctcagcgac atgaatgtcg tcgggtaccc acgcaaaacg gtcggcgtgc ggtttgtcgt
      421 aaggattgtg tttctcagcc aagatgcgcg gcacgtcgcg ttgcgggccg atcggcccgc
      481 agccgagctc gacgatcttg gcacgcagcg ccggccacag ccgctgggta tggatgccca
      541 cctcgcatac ctcgccgcac cgcccgcagg tggtgcaggt atacagcgcc agcgacaatc
      601 tctccagcct cggccgatcg agcgccggcc ggccaaatag gcgcgccacc gggttctcct
      661 gcgcgcgcac cagattgccg tactcgcgga ttttctccgg tgggctgatg gtttcgtcgt
      721 ggagaatgtc gaacaccggg caccaatcga gacaggccca gcagcgcgtg caggcatcca
      781 ggctgacgag ctgcagtttg gtcaagccgt ctgcaaggga gattggcttg agggccgcct
      841 caatcatccc acaccttcct ttccgctcac agctactcgg cgttggcggg ccgcggcggt
      901 ggtgatctgg gcggcaaaaa ggtgaaagag tttggagaaa ggcagatagg cgatcaacgc
      961 aaagccggcc gcagcgtgcg ccaggtaagc cgctgtgtac caacgcgcgt agagcgagcc
     1021 gcctccggcc aatacacgaa ggatctcgcc gaacaatgcg ccgacgggcc aatactgcgc
     1081 cagcagccac cctgagccgg acagacgcac tccctccagc acaaagccca acagcacggt
     1141 gacggcaaag agccacagca ccacgccgtc cgccgcaacc gagatggttt tcacgggccg
     1201 gaagagatag cggcgggcga gggccatgag cagcccgacc aggagcaatc caccggcgat
     1261 gtccatcacg ggcgcaagga ggaactgcag gcggtcaatc gacgcctgcg gcaggatgac
     1321 ttcggtcaga gagataagaa ctccgagcca cagcacgacg aaactccaac tgatcagcat
     1381 gagcatcacg cctcgcacct tgcttcgggc gaacacccgt cgggcgaaga gacagtcggg
     1441 ggacagcgcc tggaacagcg tcagccacag ggcaccgagc agtccccggc ctgcaatcgg
     1501 cccgctgtcc ttatcctcac ctcgcagcca gatggacatg cgaccgagcg aggccagtgc
     1561 ggaaatggcg aggacggcca gggcagcgac gttgaagatc gcccggaacg cgggaggtat
     1621 gacgatcagc gattggaaga gggtgtgcat cacacgttcc ttgccctttt gcaagggcgg
     1681 gccggagatc cccgccccgg cccgctatcg gtaatctctg gctggccttt gcgcttactt
     1741 ggcgggcaac gtttgggcat aggccagcag gttggcgatg tcctccagcg tccagcccaa
     1801 atcgatcccg gccggcatgc tcgtgcccgg ctggccgaac cacgccttgt ggaagaactc
     1861 ccacgggttg tcgctggcga tcgtgcccac gtactccgga ttggcttcat cgccgaagtt
     1921 gagcgtcttg ccgtcctcgc cgtggcaggc cgtgcatgtg tcgtcgaacc gtgtcttgcc
     1981 ctctgccggg tcgccgttta ccgtcttgtc ggcgttgacg taggcagccg tatctaccaa
     2041 accgtcccgc acgaaggccg ccagtgcatc gatctgatac tcttccagga ccgccgagaa
     2101 gtcgtgatcg gggttggctg tgcccgtcag ccaggccgcc agttcctcgg ccgacaaaga
     2161 agccgaagct agaatgccct tgaagccggt cttgtgagag ccggagccat aggcgccctc
     2221 gacgcccttg taatcccagc catggcactc cttgcagcgc caggtgtcgg cgccggtgcg
     2281 cgtgttggtg gtctgcgtct tccacagcgg ctggtcggtc gccggctcgt ccgcgccggt
     2341 cactgcccac cacttgtcgt acagcaggcc gcccaggctg gtggatggag ccgtcggcag
     2401 ggtttgggcg taggccagca ggtcggcgaa gtcttcctgc tcccaaccct cggctatgcc
     2461 ggaaggcatc tccgtgcctg gctggccgaa gcggactttg tggataaatt cccacggatt
     2521 atcgctggcg atggtgccga cgtattccgg ctcggccgta tccccgaagt tgatcgccgt
     2581 gccttgaggg ccgtggcaag tgacgcaggt gtcttcgaat accaccttac ctgtttcggg
     2641 gttgccgccc gtgggcgtct tatcagcact caccagatcg gccgcgtcca tctgcgcctg
     2701 ggctacgaac agcgccaggt cggtgagggc ttgctcgtcc atcaccgccg agaaatcgtg
     2761 atcgggattg gtcccgccct tgaggctggt caggatctcg aatgggtgct tgtctctggc
     2821 cgcgtagatg cccttgaagc cggtctggtg ggagccggag ccataggcgc cttcgacgcc
     2881 cttgtaatcc cagccatggc actccttgca gcgccaggtg tcggcgccgg tgcgggtgtt
     2941 ggtggtctgc gtcttccaca gcggctggtc ggtcgtcggc gtatccgcgc cgatcaccgt
     3001 ccaccagttg tcgtacaaca aaccgccgcg caccgcgtcg ccggtcagtt cgggcagcgg
     3061 cgtcgggctt ggggcgggcg tggccgtggg cttgggcgtg gcggttggcg ccggcgtggc
     3121 tgtgggtgca ggcgtggtga cttgacaggc aaccagggtc aacagcaggc caacggtgat
     3181 tgccttcagg aaagacgtgg gcagggcgga tctgatctgt tccatttctc ctccaagtgt
     3241 gactagcgat ggactgtggt cacatcgaac tccttacctg cgccgtggca gacttcgcag
     3301 gtctccaggc cggaggcggt cgtctgcaaa tcaatgtggc cgcccgccgc tgcgccatcg
     3361 tgacaggccg tgcacactgc acgggtaggc aggatagtac tgaccacatt gcccgcctgg
     3421 gtgacgatgg tcggctggct gccgcgcggg agtggcaagc cgtacgtgcc ggggcggtgg
     3481 caggtttcac agtctgcaag attgccgggg aagagcaact cggtgaagtc atgagtcgaa
     3541 ccgccgaagc catagacgat gaacggcctc tgggagcgct cctcgcccag gtggatcttg
     3601 tgaatcagca ccttgaagtg gatcgacgcc ggcggcatcg cctcggcggg acgcacttct
     3661 tcgtcgctgg cggtggtgtt gtgacacatc acgcagtact cggtgttctt gcggatcgtg
     3721 ccgtggaggg cgagatcctt atggcaggca ttgcacttct cgcggtccac cacttgacgg
     3781 cgtggcgccg gctcaccgcc gtccagggct acgtaggtca ccgggttaaa gccgggcacg
     3841 cgcaccggat cggctacgcc ttcgatggtc tccatcacgt agccctccat gccaacggcg
     3901 tacgtgccgg tggcatcgtc gggcagggtg taatcgaagg tgtagctgta ggccccagcg
     3961 cctgcgtctt caacagccgg gggcgtgtcg gatggcgctc ggaagatcgt ctcggtcacc
     4021 cgggtgacgt aatcgctggt cgggccggcc aggttcaccg ccaggtagtc catgtctgcc
     4081 ggcgcgatgg cagcgccact gttgtccgtg accttgaaag tcacgactgg cgacccgcct
     4141 ggcgcagcgc cttcgacgct gacgatctcg aagttgacac ccttgatctg ggttgagttg
     4201 ctcgggatag tgtgcgcgcc ggtgatcgaa gcgtcaaact cctctccgtc gggcgcatgg
     4261 cagttggcgc acgtgccatc ggcctgtttg ccgccggggt gattctcgcc ggtctccagg
     4321 tttacatcgt cgtggcaggc agtgcagacg gcgatttgcg gcttcgtctt gtagttgtcg
     4381 ctgtcggcgc cgcccgtatg gcaggttgtg cagttgcgca cgtcctgtgg ccaggcgaca
     4441 tgtgagtagt catggctgga ctgccgaaag ccgatgatct cgtacggctg gccagccagc
     4501 acgctgggca ggaactcgcc gcggtgaatc ttgtggatga acacgcggag attgagcacg
     4561 ttgcccgttt cagggtcgat gttctggtca gtgtggcagg tgacacacag cacggtttcg
     4621 cgccgcacgc cgccgtggat cgccagcggg ttgtgacagt tattgcagtt ctgagtcgca
     4681 accacttcgc gcgtcacgct gggtgcgcca ccggccggca cgaaggtgaa gacgtcgttc
     4741 gccacgctcg cgcgaccgtc cttgtaggca tacagcccca ccgatgtggt cagggaagga
     4801 tcaacttcgg atgtcagggt attggtgaag gtgtaggtgt attgtccatc gccgacggat
     4861 tgccatgcgc caccgctctc ggcgaatggc tgagtcgcct tcgccaacgc cggttgctgg
     4921 gtttctccgc cgacggtgaa cggctggccc tccacctcgc taaccagcag gttttgatag
     4981 ttactcaaac cggtgtctgc atccaccacg atctgcgcga cagtgaaacc gtagccctcg
     5041 agcgcctcgg gtttctgtgg acgaccgtct gcgtcggcga gtgtcagaga gacgaccggc
     5101 ttgccgtcgg cgggaaactc gacgctcgtg atctcgatat tcagcccagg gccggggacg
     5161 acgaatgctt gcccttgcgg cccggccggc ccggccaggc cttgaggacc tgacgggcct
     5221 tgaggaccag gtggcccagc ttccccgggt gcgccggcgc acgcggtcag ggtcagagcc
     5281 agcccgaggc caagcagccc cagactggcg agtcgaatgg cgtgaccttt catggcaccc
     5341 ttcttgtggc tattcacctt ggatcaggcg aagcaagtct tcaggctgtt caatcgtgcg
     5401 ccgctcgcgg tggtacacgc tcaatttgtt gtcggcaagg ccgaggctga tcatgcgcat
     5461 gttccaggga atcgtctcca ggatctccat gacctcgggt ggaacactgc cccctacctc
     5521 ctcaacaaga accgtgtccg gccgcaagtc caagatcgcg tccttcgcag ccgcataatc
     5581 agaggtcgcg cctgtccatt caatatcagg gcgattcaag agcaggcgca ccgactcgtg
     5641 aaagatgggg tgagcccaaa tcgcaaatac ccgccggatt gccatgtggc cccgctaaat
     5701 ctctcgctga tacgttagta cagaatgcgg cgtttgcata tcggggagag cgccaattgt
     5761 gacaaaaaaa acgccaaccc ggttggcgtt ttgtgcgagg gagtaactac tcagccgtca
     5821 ttgcgacccc gctgagaagc gcggggcagg tgagcgccgt ttgcgacgaa gcaatctccg
     5881 ccgcccttag cgaacccggc tgtgtcgaag gggcagtcga aggaagcgct cggaatgacg
     5941 cgggttgagc agttacctac acgcttattc agaagtcacg gatcagaccg cgctcgcggg
     6001 cgtaccggat caggtcgtgg cggctactca ggtcgagttt tcgcatcaag tttgtgcggt
     6061 ggctgtgaat ggtgctggga ctgacgatca agcgctcggc gatctcttta ttgctgtacc
     6121 cttctgccaa caagcgcagg atttctcttt cgcgtggcgt gagcgtcggt tggggcaaac
     6181 ctgccccctc cttcaggcga ttgaggtaat cctgtaggag ttgcttggcc atcgttgggt
     6241 atagaaacac ctccccccgc gccaccgccc gaatagcgcc gatcagctcg ttcgtctccg
     6301 cccctttcag cacgtagccg gaagcccctg ccttaagcat ctcaaagaaa tactcgtccg
     6361 agcggtgcat agtgagcacg agaacatgca tgtccggaaa gcgcgctttg atctggcgcg
     6421 tggcctccag cccatccatg cccggcatgg cgatgtccat caggaccatg tcgggccgga
     6481 gcgactctgc cagcgccacg gcttcgtcgc cgttcagcgc ttcgccgacc acctcgatat
     6541 caggctcggc ctgcaacagc agacgcacgc cagagcggac gatggtgtga tcgtcggcga
     6601 tcagtacctg gactttgtca gccaccgtct gcctccggga gcgggattcg aatccggacc
     6661 cgggttccgg ctccaggctg cgacaggatg tcgagtgtgc cgccgcactg tgccacccgt
     6721 tcctccatgc ctcgcagccc taagccgcgc gggccgtggc cgtttgcctg aacttcgcgc
     6781 agatcaaagc cctggccgtc gtccgcgatt tctccctcga acgagccgtc gcgcgctgcc
     6841 agtgacacgc acaccttgcg cgctccggcg tgccgcgtga cattggtcaa cgcctcctgg
     6901 aaaatccgga acagcgcagt ctcgatctcc agcggcagcc ggcgtgtgag gtcgcgagac
     6961 tcgagttcga aatgaacgcc ggtgtccttc agcgcgcgtt cggcgtgagc ccgcagcgct
     7021 ggcgccaagc ccaggtcgtc cagtgccgac ggacgcagag acaagatcat gacatacgcc
     7081 cgctcggtcg cctcagtgat tagggcacga gcttgttgga gttgtacgcg ggcctgctcg
     7141 ggttgatgga ccgcctgctt ttcgatagct tcgagacgga gcgccagacc ggccaggtcc
     7201 tggccgagac cgtcatgcag gtcacgcgct acccgcctgc gttcatcctc ttgggcggcg
     7261 atcagccgct tgagtagttc gtagcggtgg cccgcttcgc ggcgcagacc ttcagagagc
     7321 gcgcgattct cggcttcctc agattgaatc cgtcgcccca tttcgttgaa cgcggccgcc
     7381 aattgcccga tctcgtccgg gttgttggct ggcagccgca ggcctagttg tccccgacca
     7441 aaacgcgcca gggcttgcgc caccgcttcg agacggcgga tcacgagcga gctcatggcc
     7501 aggttgacga cgatcactgt cgtcagaatc gtcccccccc accacagaag gttctcttgc
     7561 agactggcgg ccaggggggc ttccagtggc gccatgggga tgtcggtgag caaaaggccg
     7621 ttcaagcgtt ggccagggtc atggcaggcc tggcacgctg gccgattctc aatcgggttc
     7681 atgctgcgga aaacgcgctg accatcagat agggtgatca caacgctgcc cgggcgttta
     7741 gccgccggca gctggtggca ggtctggcaa tttggctcac gattgtccaa ttgtgcgcct
     7801 acgccctggc ccttggctgc gaagatgacc cgacccgacg tatcgagtag gtagatgacc
     7861 cgaacggtct cgccttcacc gatcgcatcc agcacgtgtt ggagcccgtc caagtcctgg
     7921 gtcagcatcg catgctggag gctgttttcg atgacttggc cggtctgtgc ggcgaggaac
     7981 gacagattgg cgagcaccgc ttctcgctgc cgtgcgtact gaatcgctgt gaaactcccc
     8041 aggatgagaa taaggggcag caccacgcca agagtgactt tcgctctcag gctgtcacgc
     8101 acgaaacggg gaaacacgtg gcccgctgac attcgcccac ctctcacgcc accaacatag
     8161 caaggttccc agccgccatt aagtattgat tgtcacactc aacgtgcatc cccataacct
     8221 atccgcatcg gcatggggat gcactgtggg aggcatctcc agatgccgag ggggtcaatt
     8281 atcgggagcg ccagcggcga tccaatcgag tatgacctgg atttcatcct caggcagcag
     8341 gccgccggga ggcatcatag tgccctgcgt ctgtgtgccc cgaatcttct gggccagcaa
     8401 actgccgtcc acatccccgg cgatcacggc gggcgcatgc tcgcccgagc tcatcacgga
     8461 ttcgtagctc gaggaatccc atccgccgag gctgccgtgg cagacggcgc acctggcgtg
     8521 gaagatgggc agcacgtcgg cgctgaagga agggatacct gccggccttc cctgttctgc
     8581 gcttcccagt tgtcgaagga agtccactag ctgctcgatt tgctcggaag tgaggacctc
     8641 gccccaggcg atcatcggcg tggctgagtg ccccagattg atcgtgtcga agagctcctg
     8701 gtccgtgttc tccgcctgga actccggatc gcgcagcgat ggccctatgc cgccttcccc
     8761 attcgcgccg tgacactgtg tgcaaaggtc tgtgtagacc tcggatccgg agagagagag
     8821 ccgcgtggca gcgacctcgg gcgggagttc cactggcggg tttgcctccc acgatcgaaa
     8881 gtaggcgacg atggcttcga tctcgtcgtc ttccagaggg ccgccgaatg ccgtgccaaa
     8941 cggcgacatg ccgaagttcg gctggccctg ggcaacgacg gaacgcaaag tggcgtcgtc
     9001 gcgcgtcttc aagtattccg ccgagctgat cggagcgatc acgtcgtcgg ggcgagtcgg
     9061 attgggaccg ccttcgccaa gctcgccatg gcaagtcgca cagttctctg cgaaaagctg
     9121 ctgcccgagc gcgatcggcg cgccgctcgc cgcctgcaag gtatagacca ccagggcatc
     9181 caactgttct ggcgtgagca cgcttcccca gaccggcatg gtttcgtgcg cgccgccgct
     9241 ggcgatcgtt tctcgcgcct gggcgacgtc ggcggcggac ggcacgcgct ccccgtggca
     9301 gctcgagcag tactgcgcga acacgcctgc gccttcagga gtcgagcctg gatcgacgac
     9361 gtatcgggcg agtgtatcca gctcggaatc gctcaatctc tctcgccacg gcggcatggt
     9421 caggtgcagg ccgccctggc tgatgatgcc gcgcagctcg ctctcctcgc cagagaaggc
     9481 gacggcccgg ccgtggcagg aggcgcagtt cgtcgcgaac agtcgctgcc cgtccggagc
     9541 gacgaggaaa ttaagcagct cgatgtgatc ctcgtggctc aatacctccg cccacacggg
     9601 gacctcgacg tccgcatggg ccggataggc cggaccttga tctaagacgc ttttcagttc
     9661 gcgcggatcg cccgcgacca gctcgggcgc ttcgtggcag gcgccgcagt tttgcgtgaa
     9721 tagcctgctg cctcccggcg agaggatgaa cccggccagg gcatcgatct gatcggtggt
     9781 gaggtccgcg ctccaggccg gcatgccttc gtgcttgccc tgggcaatca catcatgcaa
     9841 gttggcgccg gacgggacgt cgatcgaagg gccgtggcaa ccggcgcagt tcgccgcata
     9901 cagcgcggcg gtccggtccc cctcgacgac ctcgacaggc gggggcgcct cccggagggc
     9961 ctggacagtt aagaaggtga tacccaccac ggccagcagc gtcacaccgg tggcaacccg
    10021 tcttcccaga aagtgccggc gcggggaacg gtccaggaac ggcaacaaga acagcaagat
    10081 gaccacgaga gtggggatca cgacaacgcc gatcacttcc agctggcctg ggaagtactt
    10141 caggagctga aacagaaaga gaaagtacca ctccgggcgc ggcgtgtagg acgtgtcggc
    10201 gggatcggcg cgcgcctcca gcggcgcgcc cacgaaggtg gccagcgcca cgaggatcac
    10261 gaatacgagc agcgcgacga cggcgtcttt gatcagggca tccgggaaaa acggaacccc
    10321 gtgctctttc gcctgcttgt attgctcgag gtactcctgc ttctcttgct cgttcatcgg
    10381 ttactcccct cgcttgggta cggcgctgat tccgatgcgg acgaccagat acaggtggat
    10441 gccgatcagg atcatcaata cggccggcag catccagacg tggacgccga agaagcgggc
    10501 cagcgtcacg gcggtcagct cttcaccgcc gcgcacgatg cgcagaatgg tgtcgccgat
    10561 gacgggcggc gtgccggcga tctgcgtgcc gactgtggtc gcccagtagg ccttctggtc
    10621 ccacggcaac agatagccgg tgaagctgaa gccgagcacc accagcagga gcgcgacgcc
    10681 cgtcacccag gtcatctcac gggggaactt ataggaaccg ttcaagatga cgcgcagcat
    10741 gtggagcacc gccagcacga ccatcgcact ggctccccag tgatggagac cgcgtatcag
    10801 ccagcctgcc gagacctccg ccgtgatata ctgcacgctg tcgtaggcct ggtccggcgt
    10861 ggggacgtag tagatggtca gcaagatgcc ggtgacgatc tggttgatcg cgacgaagag
    10921 cgtggcgctg ccgagcgtat acagccagtt gaccttgggc acctggcgca ggaagatcgc
    10981 ctcccacgcc cgacgccagc ctgagcgctc gtcgagccaa ttccctaacc gcgtgctcat
    11041 ctcatcctcc caagatgaat aattggtcgt cctcgacctt gacttcgtaa cgatcgagag
    11101 gcctgggggg tgggccgctg atgacgcgcc catccttagc gaagatccca ttgtggcagg
    11161 ggcagaagaa ctgcccttgc tgggcgatcc accgcacgcg gcagcccaga tgcgtgcaga
    11221 tgttcgacat ggcgatgaag tcgcgtccgt tttccgtcag gacgtagacc gtccgttctt
    11281 cttcattcac gatccagccc gtccggcgct tgatcttcgt tttgaacagc gtcggcgtgc
    11341 cgagctcgac tttcgacgcg ctgcccagcc ggatccagtc ttgggcctcc tcgcgcttga
    11401 gcgccgggcc gacgatgtag gcgatggccg gaatcgccag gccggcgctg atcaccccgc
    11461 cgatcgccca ggtcgtgagg ctcagaaact tccgacgtcc gatttttcgc tcttcgtcca
    11521 tgcctgtctt tcctctcctt ctggagtttg gccacagcca actatggcg
//
LOCUS       DBNHXB010000386        12146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3464844, whole genome shotgun sequence.
ACCESSION   DBNHXB010000386 DBNHXB010000000
VERSION     DBNHXB010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 12146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12146
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3464844"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="AC162G_15580"
     CDS             complement(<1..163)
                     /locus_tag="AC162G_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR5077276.1"
                     /translation="MSKGHFERTKPHVNVGTIGHIDHGKTTLTAAITKTLALKKMAEF
                     RSFDSIDNAP"
     gene            complement(286..2382)
                     /gene="fusA"
                     /locus_tag="AC162G_15585"
     CDS             complement(286..2382)
                     /gene="fusA"
                     /locus_tag="AC162G_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR5077277.1"
                     /translation="MARDYPIDRTRNIGVIAHIDAGKTTTTERILFYTGKTYRLGSVD
                     EGTTVTDWMEQERERGITITAAAITAIWRDVQINIIDTPGHIDFTAEVQRSLRVLDGG
                     VVVFDAVAGVEPQSETVWRQADRYRVPRICYINKMDRTGADFYRTVDMIRYRLNASPL
                     PLQLPVGRESEFDGVIDLFAMKALRWTDDLGMQIEESDVPAALLQEAREARATLVERI
                     AETDDELTHRFLEGADISNDDLKAALRRATIQSKLVPVFCGSSLRNRGVQPVLDAVVD
                     YLPSPADIPAIAGVNPYTGKTETRSAADGAPFSALIFKIVTDPYVGRLAYFRVYSGQV
                     SAGQSVTNATKGRKERVGRVLRMYADRREELDVVYAGDIGATLGLKDSFTGETLCDAA
                     HPIVLESIAFPEPVISVAIEPKTTADQDKMGVALRALSEEDPTFRVRADENTGQTIIS
                     GMGELHLEVLVDRMLREFKVQASVGRPKVAYRETITQPVRSEARFVRQTGGRGQYGHV
                     VLDLEPAEKGAGFSFENGIVGGVIPKEYIPAVEKGVREAMESGVLAGYPLVDIAVQLV
                     DGSYHEVDSSEMAFKVAGSMALKDGVQKAAPVLLEPIMKVEVVAPEEYTGDITGGLSS
                     RRGQIEGMEPHSQGVQAIRALVPLGEMFGYATDLRSMTKGRGTYTMEFAHYAEVPGSV
                     ADGILQGGRRAAVRAR"
     gene            complement(2517..2987)
                     /gene="rpsG"
                     /locus_tag="AC162G_15590"
     CDS             complement(2517..2987)
                     /gene="rpsG"
                     /locus_tag="AC162G_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR5077278.1"
                     /translation="MSRRARAIRRQIDPDLRYNSVQLQVFINHLMRRGKTSIASRIVY
                     DAFDMIEDRSKRAPLEVFEQALKNTMPSVEVKPRRVGGANYQVPVEVGSERRVTLAIR
                     WLLQAARTRAGKSMSEKLAGEFMDAAQNQGAAIKKREETHKMAEANRAYAHFRW"
     gene            complement(3005..3433)
                     /gene="rpsL"
                     /locus_tag="AC162G_15595"
     CDS             complement(3005..3433)
                     /gene="rpsL"
                     /locus_tag="AC162G_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR5077279.1"
                     /translation="MPTINQLVRKGRKTKHAKSKAPALLYTWNSFKQRRIRQPKGAPL
                     KRGVCTIVKTQTPKKPNSALRKVARVRLTNGIEVTAYIPGEGHNLQEHSVVIVRGGRV
                     KDLPGVRYHVVRGVLDTAGVEKRQQGRSKYGVKRPKAAKK"
     gene            complement(3610..7521)
                     /gene="rpoB"
                     /locus_tag="AC162G_15600"
     CDS             complement(3610..7521)
                     /gene="rpoB"
                     /locus_tag="AC162G_15600"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR5077280.1"
                     /translation="MNRTTGAKSYARIADVLTLPNLIEIQLDSFNWFVSEGLRDLFDE
                     ISPIESFDGSLKLYFPGHSEEAKQFKLDYWFEDPAFSQQECMERDLTYGRGLYINVAL
                     VNKRTNEHVVNDIFLGEFPWMTENGTFIYNGTERVVVSQLIRSPGVYFDLESDPASGR
                     VLAQAKLIPDRGAWMEFETRKSDYITLKFNRKRTVPVTILLRALAAIKDETGVAVLQA
                     GSDEEILDLFKDVDTNPDHPYILTTLRQEGALQPRERRTIAEEALLEFYRRMRPGDPP
                     TLDNARSYLKDQLFDRRRYDLERVGRYKLNQRLGLQIPHPIRTVTKQDLIKLVEHMIR
                     INNGLEAADDIDHLGNRRVKTVGELIQNKLRIGLRRMERVVKERMSIRDADQMTPVTL
                     VNIRPVVAAIREFFGSSQLSQFMDQTNPLAELTHKRTLSALGPGGLRRERAGFDVRDV
                     HHSHYGRICPIETPEGPNIGLIGRLATYGRVNPFGFIETPYRKVLRGLPHTDERLIGS
                     AVRSDVTDPSTGEIVAPKDTRVTEDLFKRIAKLQLEEEIAIVPRVSDEIAYMSADTED
                     RFIIAQANALMNEHGEFVSPRISVRHYQGFATEPPERVDFMDVAPRQIVGISAALIPF
                     LEHDDANRALMGSNMQRQAVPLLQPDVPIVATGMESQAALDSGQVVVAEEDGDVVGVT
                     GREITIQTKKGRKVYTLRKYNRSNQSTCIDQRPIVHKGQKVKRGQVIADSSSTNSGEL
                     ALGHNVLCAFISWEGGNYEDAILISERLVREDQFTSIHIEKHEVEARDTKLGPEEITH
                     DIPNVGEDALKDLDENGIIRIGAEVGPNDILVGKITPKGEKELSPEEKLLRAIFGEKA
                     REVKDSSLRLPHGEHGKVVDVKVFTRDEHRDLAAGVDKMVRVSVAQKRKLTEGDKMAG
                     RHGNKGVISKVVPIEDMPFLEDGTPVDIILSPLGVPGRMNIGQILEVHLGWAANRLGF
                     RAITPVFDGADESEIEGELARSWLVDRAWSDLARRAWEWLREQEVNTDELRDDTEAQL
                     LYLTALVEERGYDVERLRFDETYARRAFLREWLIEQGHDPDQVLSFEGDARPHAERAA
                     SDRLAVQTCLQMWLADRGVNTDGLSLAQLEERASQVAVELGEPQPTVGKMILYHGKTG
                     EPFDQPVTVGIIHMMKLAHLVEDKVHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVW
                     ALEAYGAAHTLQEMLTIKSDDVQGRVKAYESIVKGEPIEEPSVPASFRVLVKELQSLG
                     LAVEAITEAGEVVKFGKEEEKQRTPRLGVGLMGFDDDYGGS"
     gene            complement(7772..8890)
                     /locus_tag="AC162G_15605"
     CDS             complement(7772..8890)
                     /locus_tag="AC162G_15605"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR5077281.1"
                     /translation="MRYGRIAGWGKYVPPKVLTNLDLEKMVHTSDEWIVTRTGIRERR
                     IAGDGETTLTMSVAAARQALERAGLRASDLDLILVATSSPDYLLPPVSSMIQDRLGAR
                     CGAFTLVAGCTGFVYGLATAQQFIASGAYRTILVIGAEIISRHVDWTDRNTCVLFGDG
                     AGAVVLQAVDAPTGVLSFVLGSDGAGAMHLMVPGGGVAMPFSQRVIDEGAHYIRMNGR
                     EVFKFATRVMAQAAIQAVEKSGLTLNDIDLLIPHQANARIIEVAARQLGLPMDKVFVN
                     LDRYGNTSTASIPIALVEAIEQGRCADGSVVLMVGFGAGLTWASAVVKLGTLEQTRYA
                     LWRTIPLRARTRARAAVGALSMRAGALLLPLYARVTRR"
     gene            complement(8887..9273)
                     /locus_tag="AC162G_15610"
     CDS             complement(8887..9273)
                     /locus_tag="AC162G_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077282.1"
                     /translation="MPSQKRSSSSQRRGDTPGGPSRPGMPARARRASPVRASLGMEQR
                     VRDVLIPVEPSQQFVRDLGRGLGVAARRSRQSLVQRYRTGILIGAAIFGSLASVVGVV
                     VLILRQRARVRGVLAHSQVFTQEGGA"
     gene            complement(9388..9987)
                     /locus_tag="AC162G_15615"
     CDS             complement(9388..9987)
                     /locus_tag="AC162G_15615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5077283.1"
                     /translation="MSEPLDDMELVARVAEDPSAFGELYERYVTKIYNYVYYRTGNYH
                     DAEDLTGRVFYRALMHIDRFVDRGVPFSAWLYRIAHNLVANWHRDHARRQEYSLEDLT
                     LTSPARDTPEATTEANEERLRLLQAIRNLPPERQELLILKFVEHLSNADIGQIMGRSE
                     GAIKSLYHRTLLSLRDELATSAVDDLAGSVDPSSRKEHP"
     gene            10176..11453
                     /locus_tag="AC162G_15620"
     CDS             10176..11453
                     /locus_tag="AC162G_15620"
                     /inference="COORDINATES: protein motif:HMM:NF024183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HIR5077284.1"
                     /translation="MPRQSILCPACDSPIRLGDDQCAACGQLLCPGCGAAVGPYDSTC
                     SRCGAEWTLACPDCGGEVLAADARCPHCQAILFEEEPCPHCNRMMPDDLTICPACGGE
                     RCPTCGAALDAGVMSCAACGAVFILACPNCGEEIPEGATRCPGCGASFVDAIDDAQAE
                     RCPHCGASASRLDGVCAACGRTFCPRCGEATWDADTHCRSCGLALIFNCPDCGQELLA
                     SAPQCPSCDRLFARECPSCHRAIFGAPLICSGCGNALPAEKASTTRVTFDRPGLASPI
                     RFERCPHCGERTERASGWCPACRTAFCPNCLAITLEGEDVCVRCGHLLPGVEYACPRC
                     QSSVPASIDVCPACGLDLSGRCPSCGADLPDGAAVCPECMQLVCPECGSAVQAGTLAC
                     PVCRAELAIPCPSCGARLSLHAETCPSCKARIA"
     gene            complement(11600..>12146)
                     /locus_tag="AC162G_15625"
     CDS             complement(11600..>12146)
                     /locus_tag="AC162G_15625"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5077285.1"
                     /translation="AAQAMMAAIGVEWGGVDVLVNCAGWFESGLRVESIPPDKWRRMM
                     DTVLGGAYHTVGLCAPHMRLRKWGRIVNIAARAGLVGGVHMAHYAAAKAGLIGLTRAL
                     AKELGPSGILVNAVAPTRILTPRERERLSLDEQERIARRIPLGRIATPADVARVVLFL
                     GSGWNTYVNGEVITVGGGFRE"
BASE COUNT         2036 a         4182 c         3658 g         2270 t
ORIGIN      
        1 ccggcgcgtt gtcgatcgag tcgaacgacc gaaactccgc catcttcttc agcgccagcg
       61 tcttggtgat cgccgccgtc agcgtcgtct tgccgtggtc gatgtgcccg atcgtcccca
      121 cgttcacgtg cggcttcgtc cgctcaaaat gccctttcga catggcttac cgcctcctga
      181 aaaatgcgtt aaaacctatc cgcaaacgta ggcagggccg cttcgcgcca tgctccgccc
      241 agccgcgcat agggatgcgc ggctacgggg ttttcggata ggcgcttagc gcgcccggac
      301 agccgcgcgc ctgccccctt gaagtatgcc atcggccacg ctgcctggaa cctcggcgta
      361 atgcgcgaac tccatcgtgt acgtgccgcg ccccttcgtc atcgagcgca aatcggtcgc
      421 gtagccgaac atctcgccga gcggcacgag cgcccgaatg gcctgaacgc cttgactgtg
      481 aggctccatg ccttcgatct ggccgcgccg tgatgagaga ccgccggtga tgtcaccggt
      541 atattcttcc ggcgcgacca cctcgacctt catgatcggc tccagcagca ccggcgccgc
      601 cttctgaacg ccatctttca gcgccatcga gccggcgact ttgaaggcca tctcgctgga
      661 gtccacttcg tgatacgatc cgtcgacgag ctgcaccgca atatcgacga ggggatagcc
      721 ggccagcacc cccgactcca tcgcctcgcg cacacccttc tcgacagccg ggatgtattc
      781 cttcgggatc acgccgccga cgatgccgtt ctcgaatgag aagccggcgc ctttttcagc
      841 cggctcaaga tcgaggacga cgtgaccgta ctggcctcga ccgccggtct gccgcacgaa
      901 ccgggcttca ctgcgcaccg gctgcgtgat ggtctcccga taggccacct tcgggcgtcc
      961 cacgctcgcc tgcaccttga actcgcgcag catgcggtcg accagcactt ccaggtgcaa
     1021 ttcgcccatg ccggagataa tcgtctgccc ggtattctcg tcggcacgca cgcgaaaagt
     1081 cgggtcctcc tcggagagcg cccgcagcgc gacgcccatc ttatcttgat cggccgtcgt
     1141 cttcggctcg atcgccacag agatcaccgg ctccgggaaa gcgatgctct ccagcacgat
     1201 ggggtgcgct gcatcgcaca gcgtctcgcc ggtgaacgag tctttcaggc cgagcgtcgc
     1261 gccaatgtcg cccgcataca ccacgtcgag ctcctcgcgc cggtcggcat acatgcgcag
     1321 cacgcgcccc acgcgctcct tgcgcccctt ggtggcattc gtgaccgact ggcccgccga
     1381 aacctgtccg gagtaaacac ggaaataagc cagccggccg acgtacgggt ccgtcacgat
     1441 cttgaagatc aacgccgaga acggcgcccc gtcggccgcc gatcgagttt ccgtcttgcc
     1501 ggtgtacgga ttgacgccag cgatggctgg aatgtccgcc ggcgacggta aataatcgac
     1561 caccgcgtcg agaacgggtt ggacgcccct gtttcgcagg ctggagccgc aaaacacggg
     1621 gacgagcttg ctctggatcg tcgccctgcg cagcgccgcc ttcagatcgt cattcgagat
     1681 gtcggcgccc tccaggaagc gatgcgtcag ctcgtcatct gtctctgcga tgcgctcgac
     1741 cagagtggcg cgcgcctccc gagcttcctg aagcagcgcc gccggaacgt cgctctcttc
     1801 gatctgcatt cccagatcgt ctgtccagcg cagcgccttc atcgcaaaca gatcgatcac
     1861 cccgtcgaac tcgctctcgc gcccgacggg cagttggagc ggcagcgggc tggcattcaa
     1921 gcggtagcgg atcatgtcga ccgtgcgata gaaatccgcg ccggtgcgat ccatcttgtt
     1981 aatgtagcaa atgcgcggca ctctgtatcg atccgcctgg cgccagaccg tctccgactg
     2041 tggctcgacg cctgcgacgg cgtcaaagac gacaacaccg ccgtcaagca ctcggagcga
     2101 gcgttgaact tcggcggtga agtcaatgtg cccgggagta tcgatgatgt tgatctgaac
     2161 gtctcgccag atcgccgtaa tcgcggccgc ggtgatcgtg atgccgcgct cgcgttcctg
     2221 ctccatccaa tccgttacgg tcgtgccctc gtcgacgctg ccgagtcgat acgtcttgcc
     2281 ggtataaaac aaaatacgct cggtcgtcgt ggtcttgccg gcgtcgatgt gggcaatcac
     2341 cccaatattg cgcgtgcgat cgatcgggta gtcccgtgcc atggtgcgac aataactcgg
     2401 ttgaaaaggc gctgcgtctc ggctttcgcc accggcagcc gacgcgtcag cgtccgttcc
     2461 ccttcgaaga ctcagggcag gctctgcgcc gacccttcga agactcaggg caggctctac
     2521 cagcggaaat gcgcgtaggc ccggttcgcc tccgccatct tgtgcgtctc ttcgcgcttc
     2581 ttaatcgcag cgccctgatt ctgcgccgcg tccatgaatt ctccggccag cttttctgac
     2641 atcgactttc cggcgcgtgt ccgcgcggcc tgtagcagcc agcgaatggc gagcgtcacc
     2701 cgccgctcgg acccgacttc gaccggaacc tgatagttag cgccgccgac gcgccgcggc
     2761 ttcacctcga ccgacggcat cgtgttcttg agcgcctgct cgaagacctc cagcggcgcg
     2821 cgcttggagc gatcctcgat catgtcgaag gcatcataca cgatgcgtga ggcgatactc
     2881 gtcttgccac ggcgcatcaa gtggttgatg aacacctgca actgcacact gttgtagcgc
     2941 agatcgggat cgatctggcg tctgatggcg cgtgcccttc gcgacatgcc ttaccctccg
     3001 cgctctattt cttcgctgcc ttggggcgct tgacgccgta cttcgagcgg ccttgctgcc
     3061 gcttctcgac gcccgccgtg tccaacacgc cgcgcacgac gtggtagcgc acgcccggca
     3121 ggtccttcac gcgtccgccg cgtacgatga cgaccgagtg ctcctgcagg ttgtgccctt
     3181 ccccgggaat ataggcggtc acctcaatcc cgttcgtcag ccgcacgcgc gccaccttgc
     3241 gcagggccga attcggtttc ttcggcgtct gggtcttgac gatggtgcat acgccccgct
     3301 tcagaggcgc tcctttgggc tggcgaatcc ggcgctgctt aaacgaattc caggtgtaca
     3361 gcagcgcggg cgccttgctc ttcgcgtgct tcgtctttcg accttttcga acgagctgat
     3421 tgatcgtcgg cacagccgaa ccctccgcaa gaaagaggcc atctggttga cgacgatggc
     3481 ctccctcgat gaaaccgata cgacactcag attgtaatca gccgcgggcg ccaatgccac
     3541 agccgagcag cacctgattg taccatggtg cgcaagtttg tcaagccagg cataacagct
     3601 tgtcgccact cacgagccgc cgtagtcatc gtcgaatccc atcagcccca cgccgagacg
     3661 cggcgtgcgc tgtttttcct cttccttgcc gaacttgacc acttcgccgg cctcggtgat
     3721 ggcctcgacg gctaagccca gcgactgaag ctccttcacc aacacacgga aggaggccgg
     3781 cacgctcggc tcctcgatcg gctcgccttt gacgatcgat tcgtaggctt tcacgcgccc
     3841 ctgcacgtca tccgacttga tggtgagcat ctcctgcagc gtatgcgccg cgccgtacgc
     3901 ttccagcgcc cacacctcca tctcgccgaa gcgctgcccg ccgaactgcg ccttgccgcc
     3961 caacggctgc tgcgtcacga gcgagtaagg ccccgtgctt cgcgcgtgca ctttgtcttc
     4021 gaccagatgc gcgagcttca tcatgtgaat aatgccgacg gtcaccggct gatcgaacgg
     4081 ctcgcccgtc tttccatgat acagaatcat cttgccgacc gtcggctgcg gctcgcccag
     4141 ctcgacggcg acctgcgacg cccgctcctc caactgcgcc aaagataagc catcggtgtt
     4201 tacgccgcga tcggccagcc acatctgcaa gcacgtctgg acggccaggc ggtcgctcgc
     4261 ggcgcgctcg gcgtgcgggc gggcgtcacc ctcaaaagag agcacctggt cggggtcgtg
     4321 gccttgctcg atcagccact cgcgcaagaa ggcgcgccgc gcgtacgtct cgtcgaaacg
     4381 caggcgctcg acgtcgtaac cgcgttcttc gaccagcgcc gtcagataca aaagctgcgc
     4441 ctccgtgtcg tcgcgaagct catccgtgtt gacttcctgc tcgcgcagcc actcccacgc
     4501 ccggcgcgcc aggtcgctcc acgcccgatc gaccagccac gatcgggcca gctcgccttc
     4561 gatctcgctc tcgtccgcgc cgtcgaagac aggcgtaatc gcgcggaatc ccaggcggtt
     4621 cgcagcccaa cccagatgca cttcgaggat ctgtccgatg ttcatgcggc ccggcacgcc
     4681 cagggggctg aggatgatgt cgacgggtgt gccatcctcc aggaacggca tgtcctcgat
     4741 cggcacgacc ttcgagatga cgcccttatt gccgtggcgt ccggccatct tgtcgccttc
     4801 ggtcagcttg cgcttctgcg cgaccgacac gcgcaccatc ttgtcgacgc cggcggccag
     4861 gtcgcggtgc tcatcgcggg tgaacacctt gacgtctacg accttgccat gctcgccgtg
     4921 cggaaggcgc agcgaggagt ctttgacttc gcgtgccttc tcgccgaaga tcgcgcgcag
     4981 cagtttctcc tcgggcgaca gctctttctc gcccttcggc gtgatcttgc cgacgagaat
     5041 atcgttcggc cctacctcgg cgccgatgcg gataatgccg ttctcgtcca gatccttcaa
     5101 ggcgtcctcg ccgacgttcg gaatgtcgtg cgtgatctcc tcaggcccca gcttggtgtc
     5161 gcgcgcctcg acctcgtgct tctcgatatg aatcgaggtg aactggtcct cgcgcaccag
     5221 ccgctcgctg atcagaatgg cgtcctcgta gttgcctcct tcccacgaga tgaatgcgca
     5281 cagcacgttg tgcccgagcg ccagctcgcc gctgttggtg ctggacgaat cggcgatgac
     5341 ctggccgcgc ttgaccttct ggcctttgtg cacgatcggg cgctgatcga tgcacgtgct
     5401 ctgattgctt cggttgtact tccgcagcgt gtacaccttg cgccccttct tcgtctggat
     5461 ggtgatctcg cgtccggtca cgccgaccac gtccccgtct tcttcggcca cgaccacctg
     5521 gccggaatcg agcgccgcct gcgactccat gccggtggcc acgatcggca catcgggctg
     5581 gagcagcggc acggcctggc gctgcatgtt cgaccccatc agcgcgcggt tggcgtcgtc
     5641 atgctcgagg aacgggatca gcgcggcgct gataccgacg atctggcgcg gcgcgacatc
     5701 catgaaatcg acccgctccg gcggctccgt cgcgaagccc tgatagtggc gcaccgaaat
     5761 gcgcgggctg acgaactcgc cgtgctcgtt catcagcgcg ttcgcctgcg cgatgatgaa
     5821 gcgatcttct gtgtcggccg acatataggc gatctcgtcg gacacgcggg ggacgatggc
     5881 gatctcctct tcgagctgca gcttggcgat gcgcttgaag aggtcctccg taacacgcgt
     5941 gtccttgggc gcaacgatct cgcccgtcga cggatcggtc acgtcgctgc gcacggcgct
     6001 gccgatcagc cgctcgtccg tgtgcggcag gccgcgcagc actttgcgat agggcgtctc
     6061 gatgaatccg aacgggttca cccggccgta cgtcgccagg cggccgatca ggccgatgtt
     6121 tgggccttcc ggcgtctcga tcggacagat gcggccataa tgcgagtggt gcacgtctcg
     6181 cacgtcgaag ccggcgcgct cgcgccgcag gccgcccggt cccagcgccg acagcgtccg
     6241 cttgtgagtc agctcggcaa gcgggttcgt ctgatccatg aactgcgaca gttgagacga
     6301 gccgaagaat tcgcggatgg ccgccaccac ggggcggatg ttgaccaggg tgaccggcgt
     6361 catctggtcc gcgtcgcgaa tgctcatgcg ctctttcacc acccgctcca tgcggcgcag
     6421 gccgatgcgc agcttgttct ggatcagctc gcccaccgtc ttcacgcggc ggttgcccag
     6481 atggtcgatg tcgtccgcag cctcgagccc gttgttgatg cggatcatgt gctcgaccag
     6541 cttgatcaga tcctgcttcg tcaccgtgcg tatcgggtgc ggaatctgca gacccaggcg
     6601 ctggttgagc ttgtagcggc ccacgcgctc cagatcgtag cgccgccgat cgaacaactg
     6661 atccttgaga tacgatcggg cgttgtcgag cgtgggcgga tcgcctggac gcatgcggcg
     6721 atagaattcc agcagcgcct cctcggcgat cgtgcgccgc tcgcgcggct ggagcgcgcc
     6781 ctcctggcgc agcgtggtca gaatgtaggg gtgatcgggg ttggtgtcga cgtctttgaa
     6841 caggtccagg atttcttcgt cgctgcccgc ctgcagcacg gcgacgcccg tctcatcctt
     6901 gatagccgcc agcgcgcgca gcaagatcgt cacggggacg gtgcgctttc ggttgaactt
     6961 cagcgtaatg taatcgctct tgcgcgtctc gaattccatc cacgcgccgc gatcgggaat
     7021 cagcttcgcc tgggcgagca cccggcccga cgccggatcg ctctccagat cgaaatacac
     7081 gcccggcgag cgaatcaact gcgacacgac cacgcgctcg gtcccgttat agatgaacgt
     7141 gccgttctcc gtcatccacg ggaactcgcc caggaaaatg tcgttcacga catgctcgtt
     7201 cgtccgcttg ttgaccaggg ccacgttgat gtacagaccg cggccgtagg tcaggtcgcg
     7261 ctccatgcac tcctgctggc tgaaagccgg gtcctcgaac cagtaatcca gcttgaattg
     7321 cttggcttct tcgctgtgcc cggggaagta cagcttcagc gagccatcga agctctcaat
     7381 cggcgagatt tcatcgaata ggtcacgcag cccctcgctg acgaaccagt tgaacgaatc
     7441 gagctgaatc tcgatcaggt tcgggagcgt gagcacatcc gcgatgcgtg cgtagctctt
     7501 agcgccagtc gtgcggttca tgcaatcgat cctcccaatt ggacaaacac aacaaagggg
     7561 acttgaatcc ccttcacatg ggcaatctgc tcccgccgga tggatcatcc ggcggctggg
     7621 agcggcggat gaatcatccg ccgcgagctt tgttccagtc gcaaagtgtt attataccct
     7681 tcttcacaaa aactgtcaaa atcatgagcc taaaacaacg agccggggag tatcgacact
     7741 ccccggctcg ggtctgtcgc gccgcgcagg ttcagcgccg tgtcacgcgc gcatagagcg
     7801 gcaagagcag cgcgccggct cgcatcgaga gcgcgcccac cgccgcgcgg gcgcgggttc
     7861 tggcccgtag cgggatggtg cgccacaggg cgtagcgcgt ctgctccagc gttcccagct
     7921 tcacaaccgc cgaggcccac gtcaggccgg ccccaaaacc gaccatcagg acgaccgagc
     7981 cgtccgcgca acggccttgc tcgatcgctt cgaccagggc gatcgggatc gacgccgtcg
     8041 aggtgttgcc gtatcgatcc aggtttacaa acaccttgtc catcggcagt cccaactggc
     8101 gagcggccac ttcgatgatg cgcgcgttcg cctggtgcgg gatcagcagg tcgatgtcgt
     8161 tcagcgtcag gccgctcttc tccaccgcct ggatggcggc ctgagccatc acccgcgtcg
     8221 cgaacttgaa gacttcgcga ccattcatgc gaatgtagtg cgcgccctcg tcgatcacgc
     8281 gctggctgaa cggcatggcc acgccgccgc cgggcaccat gagatgcatc gcgccggcgc
     8341 cgtccgaccc taaaacgaaa gacaggacgc cggtcggcgc atcgacggcc tgcaacacca
     8401 ctgcgcccgc gccgtcgccg aacagcacgc acgtattgcg atccgtccag tcgacgtggc
     8461 ggctgataat ctcggcgccg atcacgagaa tggtgcggta ggcgccgctg gcgataaact
     8521 gctgcgccgt cgccagcccg tacacgaatc ccgtgcaccc ggccacgagc gtgaacgcac
     8581 cgcaccgcgc gccgagccga tcctggatca tcgacgaaac gggcggcaga agataatccg
     8641 gcgacgacgt ggcgaccaga atcaagtcca gatcggaggc ccgaaggccg gctcgctcga
     8701 gcgcctgccg ggcggccgcc acggacatcg tcagcgtcgt ctcaccgtca cccgcgatgc
     8761 ggcgctcccg gatgcccgtt cgcgtgacga tccactcgtc gctggtgtgg accatcttct
     8821 ccagatctaa gttcgtcagc accttcggcg gcacatattt gccccacccg gcaatgcgac
     8881 cgtatctcat gctccccctt cctgtgtgaa gacctgcgaa tgggccagca cgccgcgcac
     8941 cctcgcgcgc tggcgcaaaa tcagcaccac gacccccacc accgacgcga gcgagccgaa
     9001 aatcgctgcg ccgatcagaa tgcccgtgcg atagcgctgc acgagcgatt ggcggctgcg
     9061 cctcgccgcc acgccgagac cgcgtcccag atcgcgcacg aactgctgag aaggctcgac
     9121 cgggatcaag acatcccgaa cgcgctgctc catgcccagc gaggcgcgca ccggcgacgc
     9181 gcggcgcgcg cgggccggca tgcccggcct gctcgggcca cccggcgtat ctccacgccg
     9241 ctgcgacgat gaacttcgtt tctgagatgg catatcgggt tgcgtcctcc gttaagctcg
     9301 aaaccccacc cgcgtccctt cggctgcgct cagagcttgc cctgagcctg cgaaggggca
     9361 ggcttcgcgg ttgaagagcg ttcgctgcta ggggtgctcc tttctcgaac tgggatccac
     9421 ggagcctgcc agatcgtcga cggcgcttgt cgccagctcg tcgcgcagcg acaggagcgt
     9481 gcgatggtac aaggacttga tggccccctc gctgcgcccc atgatttgcc cgatgtccgc
     9541 attcgacagg tgctcgacga acttgaggat caggagctcc tgtcgctctg gcggcaggtt
     9601 gcgaatcgcc tgcagaagtc gcagccgctc ctcgttggct tccgtcgtgg cctccggcgt
     9661 gtcgcgcgcc gggctggtca gcgtcaaatc ctccagcgag tactcctgcc gccgcgcgtg
     9721 atcgcgatgc cagttggcga ccaggttgtg cgcgatgcga tacagccacg ccgagaaggg
     9781 cacaccccga tccacgaatc gatcgatgtg catcagcgcc cgatagaaca cccgcccggt
     9841 cagatcctcc gcgtcgtggt agttaccggt gcggtaatag acgtagttat agattttcgt
     9901 gacgtagcgc tcgtacaact cgccaaacgc cgatgggtcc tcggccaccc gcgcgacgag
     9961 ttccatatca tctagcggct cgctcacctc acgctccgtg cgcagccgcc atacagtaaa
    10021 cccccacggc tgccaatggt tacgacgggg aagtcgggcg tgagtataac aggctcaccg
    10081 gacgtgtcaa cgggcatcac cgccaatcca aactccaaac tccaaacttc aaaagcgcgg
    10141 caggctgggc gtcgatccgt gctacaatcg aaacgatgcc gcgacagtcc atcctctgcc
    10201 ccgcctgcga ttcgccaata cggcttgggg acgatcagtg cgcagcgtgc gggcaactcc
    10261 tgtgccccgg ctgcggcgcc gccgtcgggc cttacgattc aacctgctcg cgctgcggag
    10321 ccgagtggac gctggcctgc cccgactgcg gcggcgaagt gctcgccgcc gacgcgcgct
    10381 gcccgcactg ccaggcgatc ctcttcgagg aagagccttg cccgcactgc aaccggatga
    10441 tgccggatga cctcaccata tgccctgcct gcggcggtga gcgctgccca acgtgcggcg
    10501 cggccctgga cgcaggcgtg atgtcgtgcg ccgcgtgcgg cgcggtgttc attctggcgt
    10561 gccccaactg cggagaggag attccggagg gagccacacg ctgccctggg tgcggcgcgt
    10621 cgttcgtcga cgcgatcgac gacgctcagg ccgagcgctg cccgcactgc ggcgcgagcg
    10681 cctcccggct cgacggggta tgcgccgcct gcgggcgcac gttttgtccg cgctgcggcg
    10741 aggcgacctg ggacgcggat acacactgcc gatcgtgcgg gcttgcgctg atcttcaatt
    10801 gccctgactg cgggcaggag cttctcgctt cggcgccaca gtgtcccagt tgcgatcgac
    10861 tcttcgcgcg cgagtgcccg tcgtgccacc gggccatttt cggcgcgccc ttgatttgct
    10921 ccggctgcgg aaatgccctc cccgccgaaa aagcgtcgac gacgcgcgtg acgttcgatc
    10981 ggcccggtct cgcgtcgccc atccgcttcg agcgctgtcc acactgcggc gagcgaaccg
    11041 agcgcgcctc cggctggtgc ccggcatgcc gcaccgcctt ctgcccgaat tgcctggcca
    11101 tcacgctgga gggcgaggac gtctgcgtgc gctgcggcca tctcctgccc ggcgtcgagt
    11161 acgcgtgtcc acgctgccag tcgagcgtcc cagcgtcgat cgacgtgtgc ccggcgtgcg
    11221 ggctggatct atccggccgc tgcccgagct gcggcgccga cctgcccgac ggcgcggcag
    11281 tgtgcccgga gtgcatgcag cttgtctgcc cggaatgcgg atcggcggtc caggccggca
    11341 ccctggcgtg ccccgtttgc cgcgccgagt tggcaattcc ctgcccgtcc tgcggcgcgc
    11401 gcctcagcct tcacgctgaa acctgcccca gttgcaaggc tcgcatcgcc taatgctttg
    11461 accatttaat cttgcagggt tcggacggca gttgcactgc cgtccgccgc agtgcaactg
    11521 cggcgggaac tacgcaactt cagattgttg aggcactaag cacggatggc atggatattg
    11581 aggggctgcc acgcacactt cattcgcgga agccgccgcc gacggtgatc acttcgccgt
    11641 tcacgtaggt gttccacccc gagcctagaa atagcacgac gcgcgccacg tccgcaggcg
    11701 tggcgatgcg ccctagcgga atgcgcctgg cgatgcgctc ttgctcgtcg agcgacaggc
    11761 gctcacgctc cctgggagtg aggatgcgcg tcggggcgac cgcgttaacc agaatgccgc
    11821 tcggaccaag ctccttcgcc agcgcgcgtg tcaatccgat cagtccggcc ttggccgcgg
    11881 cataatgcgc catgtggacc ccgccgacaa gcccggcgcg cgcggcaatg ttgacgatgc
    11941 ggccccactt gcgcaggcgc atgtgcggcg cgcacagccc gaccgtgtga tacgccccgc
    12001 cgagaacggt atccatcatg cggcgccact tgtcgggcgg gatcgattcg acgcggaggc
    12061 cgctttcaaa ccaacccgcg cagttgacga gcacgtcgac gccgccccac tccacgccga
    12121 tcgcggccat catcgcctgc gccgcc
//
LOCUS       DBNHXB010000387         5174 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4639412, whole genome shotgun sequence.
ACCESSION   DBNHXB010000387 DBNHXB010000000
VERSION     DBNHXB010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4639412"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="AC162G_15630"
     CDS             complement(<1..341)
                     /locus_tag="AC162G_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077286.1"
                     /translation="MLINEYGLDVFTPPCDPGAERYSAIARLTIDISEVLPYLNATLR
                     GAVYYRTANALTWKKGGHNVAFHAYEIATSNVEDREAALKEIKGLIELVNRTWERRVE
                     ITPDYETHQRPT"
     gene            complement(346..519)
                     /locus_tag="AC162G_15635"
     CDS             complement(346..519)
                     /locus_tag="AC162G_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5077287.1"
                     /translation="MKNISQQLIVLREAGLVTHAREGLNIRYRLVRPELKNLLAFTDP
                     VCRRRFENGTTGK"
     gene            complement(516..827)
                     /locus_tag="AC162G_15640"
     CDS             complement(516..827)
                     /locus_tag="AC162G_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077288.1"
                     /translation="MKQIPSEGGACCLPQADGLTYIKVGARGAVVGMMNLDTIFQQLH
                     ALGRRPDEATDAELVGMARRFNYIPDRLSVEADYAVALRRAYAAFCARQEEGHERAAQ
                     S"
     gene            complement(880..1332)
                     /locus_tag="AC162G_15645"
     CDS             complement(880..1332)
                     /locus_tag="AC162G_15645"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR5077289.1"
                     /translation="MSRTATYHTTVSWKGEHWGHIVMGNGPEMDFSAPPDAQGHAGVF
                     TPEDAFVAAANTCIMMMFIWATERFKLKLLSYDCRVEGTKLIELDRTEIFTHLRFWPI
                     IKIAANGEDRSGVEARTRRALQSAQKYSLVANSVKSQIVIEPTIEIVP"
     gene            complement(1329..2066)
                     /locus_tag="AC162G_15650"
     CDS             complement(1329..2066)
                     /locus_tag="AC162G_15650"
                     /inference="COORDINATES: protein motif:HMM:NF020434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HIR5077290.1"
                     /translation="MPELPKKKVGIIACSGEEMAEGTVTRLAALKVLEQLRPADTVTI
                     CLPLFLAGGEGDRAFARFYPTIAIDGCDKRCAARATEMFSGKPAAGIVVTDLIAARGL
                     ERPEGLRRLNEAGLQAVDATARHVADMVDELLQRKWSRRTGAFVEESPVPAETQEPIE
                     ATCACGSGIPIQKISIDGQIVTLVALPLIFEQFRAAGKAPDDGILCELLQTVKVYNPI
                     PAESEEAYAAALLREYVAFCAKQAVQA"
     gene            complement(2059..2484)
                     /locus_tag="AC162G_15655"
     CDS             complement(2059..2484)
                     /locus_tag="AC162G_15655"
                     /inference="COORDINATES: protein motif:HMM:NF020434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HIR5077291.1"
                     /translation="MTDRKRVAIVPCSGIGKTYGSVSRDAAYDVTEDMRPAETQLVPL
                     SLLVLGDEAARTAVADAQAITIDGCKLACATKMVKESGGTVAQDFAVLDVYRRHKQFK
                     PQGIAELNEGGQKLAHALAEEIIIVVDTLGASSEGGQNA"
     gene            complement(2481..2930)
                     /locus_tag="AC162G_15660"
     CDS             complement(2481..2930)
                     /locus_tag="AC162G_15660"
                     /EC_number="1.4.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF013743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HIR5077292.1"
                     /translation="MPDYLETTVDKFTFRVATDRLYSREGVWALAEGNRVRIGLSDFM
                     QQRSGDVAFTEVKPVGTALAFGDEVASIETIKVDIALGSPVSGSVVEVNPALSDAPEA
                     INQDPYSAGWLAVIQATDWETDRARLLDPQAYFAVMKAEAEEEVKKP"
     gene            complement(2970..3233)
                     /locus_tag="AC162G_15665"
     CDS             complement(2970..3233)
                     /locus_tag="AC162G_15665"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024590.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR5077293.1"
                     /translation="MLDIKVLGTGCAGCLKMEQIVIEALEALGVREARVELVSEQRMI
                     DYGLLGDRAPGLLINGQLAWAGSVPTKEQVTEWLLYALNPTPA"
     gene            complement(3271..4026)
                     /locus_tag="AC162G_15670"
     CDS             complement(3271..4026)
                     /locus_tag="AC162G_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR5077294.1"
                     /translation="MLYLIIALTLFILSFVAGMMGLGVAFIATPVLGLFGFELKHVIM
                     PWSLWLNGLTAFSGAVAFARAKMVDWRTAIPLLIITTIVAPIGVWLLQFVPTNVVWWI
                     YVGVLLFLAYRMAFPPKMDDNKVPSISDGTRARGGLASAAIGVFAGFLGVGPGFLMMP
                     TLVMLGYTARIGAATNSVIVTLPSFSAFAGHLGSAQFDWLMLGLTSVTAVIGAWLGAR
                     FMAKRVKSLTLSRIFAAALALLAVQRVAILMTA"
     gene            complement(4091..4363)
                     /locus_tag="AC162G_15675"
     CDS             complement(4091..4363)
                     /locus_tag="AC162G_15675"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024590.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR5077295.1"
                     /translation="MNMLAGERRLVQIVGAGCPACRQLEADVRAWITRHRIEAQVERV
                     DDLVEILNYRLLALPGLVIDGRVILAGYPPRDRLDRVLLDTLAAIR"
     gene            complement(4419..4676)
                     /locus_tag="AC162G_15680"
     CDS             complement(4419..4676)
                     /locus_tag="AC162G_15680"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR5077296.1"
                     /translation="MLTIKVLGPGCDNCRKVEQAARQAVAMLSVEARVEKVTDRALFA
                     KYGLLATPGLVINEKLVSAGRIPSAAEVTTWIANALEQAPA"
     gene            complement(4726..5088)
                     /locus_tag="AC162G_15685"
     CDS             complement(4726..5088)
                     /locus_tag="AC162G_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5077297.1"
                     /translation="MKDLERTARLFKALMHPARLEILDILRNGEECVCHMEAMLGYRQ
                     AYISQQLSVLREAGLVQDRRDGWNMFYRVTDTQVYTLVDTARRMTGDTRAAARRKPAK
                     SAPCPCPRCNPVAEATAA"
BASE COUNT          971 a         1700 c         1490 g         1013 t
ORIGIN      
        1 gtcgggcgct ggtgtgtctc gtagtccggc gtgatctcga cgcggcgctc ccatgtccga
       61 ttgaccagtt cgatcagtcc tttgatttct ttcaatgccg cctcgcggtc ttccacattg
      121 ctggtggcga tttcgtatgc gtgaaaggcg acgttgtgcc cgcctttctt ccaggtgagt
      181 gcgttggccg tgcgatagta taccgcgccg cgcagcgtgg cgttgaggta gggcaggact
      241 tcggagatgt cgatggtcag gcgggcgatt gccgagtagc gctccgcgcc cggatcgcag
      301 ggcggggtga agacgtccag gccatactcg ttgatcagca tctcactact tcccagtcgt
      361 tccattctcg aatcgacgcc ggcaaaccgg atcggtgaac gccaacaggt tcttcaactc
      421 gggtcggacc aggcgatagc gaatgttcaa cccctcacgg gcgtgagtca ccagaccagc
      481 ctcgcgcagc acaatcaact gttgagaaat gttcttcatg attgtgccgc acgttcgtgc
      541 ccctcttcct ggcgggcaca aaaggcagca tacgcccggc gtagcgcgac ggcgtagtca
      601 gcctcgacgc tgagtcgatc gggaatgtag ttgaacctgc gggccatgcc caccagttcg
      661 gcatccgtcg cctcgtccgg tctccgtccc agcgcgtgca actgctggaa tatggtgtcc
      721 aggttcatca tcccgaccac ggcgccacgt gcgccgacct tgatatacgt cagcccgtcg
      781 gcctgaggca ggcagcaagc cccaccttct gatggtatct gtttcatccg ctgcgctcct
      841 gtccggctgg gtagcgactg ttgccgcaaa agcacggctt cacggcacga tctcgatcgt
      901 cggctcgatg acgatctgcg acttgaccga gttggcgacc aggctgtact tctgcgccga
      961 ttgcagcgcg cggcgtgtgc gagcctctac ccctgatcgg tcttcgccgt tcgcggcgat
     1021 cttgataatc ggccagaagc gcaggtgcgt gaagatctcg gtgcgatcca actcgatcaa
     1081 cttcgtgcct tccacacggc agtcatacga aagcaacttg agcttgaatc gctccgtcgc
     1141 ccagatgaac atcatcatga tgcaggtgtt ggccgcggcg acaaaggcgt cttctggtgt
     1201 aaacactccc gcgtggcctt gtgcgtcggg cggggccgag aaatccatct ccgggccgtt
     1261 gcccatgacg atgtgccccc aatgctcgcc cttccacgaa acggtcgtgt gataggtcgc
     1321 cgtgcggctc atgcctgcac cgcctgcttc gcgcaaaacg cgacatactc gcgcagcagc
     1381 gcggccgcgt aggcttcttc ggactcggcg ggaatcgggt tgtacacctt caccgtctgc
     1441 agcaactcgc acaggatgcc gtcgtcgggc gcttttccgg ctgcgcggaa ttgctcgaag
     1501 ataagcggca gcgcgacgag cgtgacgatc tgcccgtcaa tgcttatctt ctggatcgga
     1561 atgcccgatc cgcacgcgca agtggcttcg attggctctt gcgtttcagc cggcacgggc
     1621 gactcttcga cgaatgcgcc tgtgcggcga ctccacttcc tctgcagcaa ctcatcgacc
     1681 atgtctgcca cgtgcctggc tgtcgcatcg acggcctgca gcccggcctc gttcaagcgg
     1741 cgtagtcctt caggccgttc cagtccccgc gcggcaatca gatcggtcac gacaattccg
     1801 gcagccggct tgccggaaaa catttcggtg gcacgcgcgg cgcagcgctt gtcacagcca
     1861 tcgatggcga tcgtcggata gaatcgcgca aaggcgcgat cgccttcacc gccggccagg
     1921 aacagcggca ggcagatcgt cacggtatcg gcgggacgca gttgctccag aactttcagc
     1981 gcggcaaggc gcgtcaccgt tccttcagcc atctcttcgc cagagcaagc gataatgccg
     2041 actttcttct tcggtagctc aggcattctg gcctccttcg ctgctggcgc caagcgtatc
     2101 gacgacgatg ataatctctt cggccagtgc atgggccagt ttctgcccgc cctcattcag
     2161 ttcggcgata ccctgcggct tgaactgctt gtggcggcga tacacgtcaa gcacggcgaa
     2221 atcctgggca accgttccgc cgctttcctt gaccatcttt gtcgcacagg ccaacttgca
     2281 gccgtcgatg gtgatagcct gcgcatcggc gacggcagta cgcgcggcct catcgcccag
     2341 caccagcaga gacagtggca cgagttgggt ctcggccggc cgcatgtctt ccgtcacgtc
     2401 gtaagcggcg tcgcggctca cgctgccgta tgttttgcca ataccactgc acggcacgat
     2461 ggcgactcgc ttgcgatccg tcatggcttc ttgacctcct cttcggcttc tgctttcatc
     2521 accgcgaagt aggcttgcgg atcgagcagc cgcgcccgat cggtctccca atcagttgcc
     2581 tggattacgg ccagccagcc tgcgctgtag gggtcctggt taatcgcctc cggcgcgtcg
     2641 ctcagcgccg ggttgacctc gacgaccgag ccggagaccg gtgagccgag cgcgatgtcg
     2701 accttgatgg tctcaatcga agcgacctca tctccaaaag ccagcgccgt gccgaccggc
     2761 ttcacttcgg tgaaggcgac gtcgccgctg cgctgctgca tgaagtctga cagcccgatg
     2821 cgcacccgat tgccctcggc gagcgcccac acgccttcac ggctgtagag acggtcggtc
     2881 gcaactctga atgtgaactt atcgacggtc gtttcgagat agtcgggcat gctgtgctcc
     2941 tcagatgatg aattcgtgtc gggttccact cacgccggag tggggttgag cgcgtacagc
     3001 agccactcgg tcacttgctc cttcgtcggc accgatccgg cccaggccag ttggccgttg
     3061 atcagtaggc ccggcgcgcg atcgcccagc aggccgtaat cgatcatgcg ctgctcactg
     3121 accagctcga cccgggcttc gcgaactccc aacgcctcca gggcttcgat gacgatctgt
     3181 tccatcttca ggcagccggc gcagccggtg cccagcactt tgatgtccaa catggcaaac
     3241 tcctttccag tggataaacc actctcaact tcacgccgtc attagaatcg cgacgcgctg
     3301 gacggccagc agcgccagcg ccgctgcgaa aatgcggctg agcgtcaacg acttcacccg
     3361 cttcgccatg aagcgcgcac ccagccatgc cccaatcaca gccgtcaccg atgtcaggcc
     3421 cagcatcagc cagtcgaact gcgccgaacc caggtggccc gcgaacgccg agaacgacgg
     3481 cagcgtgaca atgacgctgt tcgttgccgc accgatgcgc gcggtgtagc ccagcatgac
     3541 cagcgtcggc atcatcagga agcccggccc tacgccgaga aagccggcga acacaccgat
     3601 tgccgcgctg gctaatccgc ccctcgcgcg cgtcccatcg ctgatggacg ggaccttgtt
     3661 gtcatccatc ttcggcggaa aggccatgcg atacgcgagg aacaacagca cgccgacgta
     3721 aatccaccat actacgttcg tcggcacaaa ttgcaacagc catacgccga tcggcgcaac
     3781 aatcgtggtg atgatcaaca gcgggatcgc cgtgcgccaa tcgaccatct tcgcccgcgc
     3841 gaaggcaacc gcgccggaga aggccgttag gccgttgagc cacagcgacc agggcatgat
     3901 cacgtgcttc aactcgaagc cgaacaatcc cagcaccggc gtggcgataa aggccacgcc
     3961 caatcccatc attccggcaa cgaacgacag aatgaacagc gtcagtgcga tgatcaaata
     4021 taacacggcg gcgatccttc cgtaggcagg gcgcggatga ggtcgtctgc gagatcttcc
     4081 gaaaactcga tcaccgtatt gcggccagcg tgtcgagcag aacccggtcg agtcgatctc
     4141 tgggcggata accggccaga atgacgcgac catcaatcac caggccgggc aatgccagca
     4201 gccgataatt caggatttcg accaggtcgt ccacgcgctc gacctgcgcc tcgatgcggt
     4261 gccgcgtgat ccacgcccgt acgtcggctt cgagttgcct gcacgcggga catccggccc
     4321 ccacgatctg aaccagtctc cgctcgcctg ccaacatatt cataatcttg aatccatctg
     4381 cgcgcgaaaa agccccgcgt cgaacggggc cgtcctgctc aggcaggcgc ctgctccagc
     4441 gcgttggcga tccacgtcgt cacttcggcg gcgctgggga tacgtcccgc gctgaccaac
     4501 ttctcgttga tcacaagacc cggcgtcgcc agcaggccat atttagcgaa caacgcgcgg
     4561 tcggtcacct tctcgacccg ggcttcgacg ctcagcatcg ccacagcctg ccgggccgcc
     4621 tgctccacct tcctgcaatt gtcacagcca ggtcccagaa ctttgatcgt cagcatatgt
     4681 tcactccttg tagatggatc gcggaggctt gggtcatgga cactgtcagg cagcggtggc
     4741 ctccgccacc gggttgcagc gcgggcacgg acatggcgca gacttggccg gcttgcgccg
     4801 ggcagccgct cgcgtgtcgc ccgtcatgcg ccgcgcggta tcgacgaggg tatacacctg
     4861 tgtgtcggtt acacgataga acatattcca gccatcgcgc cgatcctgca ccagcccggc
     4921 ctcgcgcaac accgacaact gctgcgagat gtacgcctgc cgatagccca gcatcgcttc
     4981 catgtggcac acgcactctt cgccattgcg gaggatgtcg aggatctcta gccgcgcggg
     5041 atgcatcagc gccttgaaca gcctggccgt cctctccagg tctttcatga aagctcccga
     5101 aatatcttca tgattccgaa taagaatcta gaccagagtg gatagacgga gaaaggcaca
     5161 gtgtcacccc tttg
//
LOCUS       DBNHXB010000388         7674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2794708, whole genome shotgun sequence.
ACCESSION   DBNHXB010000388 DBNHXB010000000
VERSION     DBNHXB010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2794708"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="AC162G_15690"
     CDS             <1..491
                     /locus_tag="AC162G_15690"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017045357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077298.1"
                     /translation="SERAQRKRAAELLAMVGLGDRLSHLPGQLSGGQRQKVAIARALA
                     NNPAIILADEPTGNLDSASGDELLSVFEQLHSQRGTTIIIVTHDAAVARRTHRILMMQ
                     DGRIVHEDIVGDAYTEDLKALARSALGRAVLGGAPLEVLSNGEREVMSRVLARTVERR
                     LR"
     gene            540..3833
                     /locus_tag="AC162G_15695"
     CDS             540..3833
                     /locus_tag="AC162G_15695"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5077299.1"
                     /translation="MSDLESQIAELRTSIAALEAQRAVLGEVVVNAALGPMREKLAAL
                     EAALQSPSFHPKESDAEGERKVVTILFADISGFTALSETLDPEQVTDIANACFQSLTD
                     AVTRYGGTIDKYLGDAVMALFGAPDAHEDDPERAIHAALEMQSALRQTISQLSPATGD
                     GSASLTTGLALHIGINTGPVVAGAVGAAGLRDYTVMGDAVNLASRLKDVAPIGHIYVG
                     PETYRQTSRLFEFAPLAPMRIKGKRDPVQIYEVRGRAASPGTMRGIEGLRSPLVGRGD
                     ELDLLKARLRSLPEGKGSVVALTGEAGLGKSRLISELHVAVHTANTYPDPGVRAGLAP
                     AQHRAGESAVLWLEGRCTSFTQSVSYSLIGQVLSSYFGVSGSLDASEALERLQEALDA
                     VLPGQGDELAPYLGTLLGWQVGDERVRYLDAEALQRQIFYSVRRFVGGLSTQTPLVIV
                     LDDLHWIDEASLDLLEYVLPLVEDHPLMMVWAFRPGTEQTGIEQLARRFAQTGSQHLY
                     QVDLRPLADDMSQALVRLLLARSDLPEPIQAAIVERAEGNPFYLEEVIRTLIESALLI
                     EKGGRWVIDPQIPDVEFRIRSALPDTLIGVLTARIDRLPEQPKRVLGIASVVGRAFSH
                     RLMTELIGSRTAVDEALAELERSGLVESGEATPLGGAQAAWGPTYSFRHVLTQEAAYN
                     MLLQRRRVQLHGEIGDALERLHRDEVDEYAVLLAYHYDRSQNDAKAIAYGLRAGRAAL
                     RVYANREAAGFFARVVNRLRESGSEDAQLAEALEALGDARSASGEVNAAIGYWQEALV
                     MQSRFEGRESVARLNRKLGNAWFTRGDRARAIETYRRGIDAVGDLPPGETLASLYAEL
                     GRVYFRAGQDAQAIDWAARALDLAESVGSAETASLALNTLGIARARGGELARGIAEVE
                     RGLQIAIDNDLPVAACRAYTNLGMLYGAVDHSRAVQLCQQGLALARKIGDLYYQSWLQ
                     SALASTYCSLVGNWERGVAAARASIDLDLQMGHRAHLAVPVVLLAQIYQCHGMLEESL
                     RAYREALALAEESGEPQMLFPCYNGLGALYLEIGDEEQATTYLDKAQQVVSEAGYSAD
                     SLFVLPFLA"
     gene            3943..4560
                     /locus_tag="AC162G_15700"
     CDS             3943..4560
                     /locus_tag="AC162G_15700"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HIR5077300.1"
                     /translation="MDTTKDEVKIGSPAPEIELESVQGPAVRLSEFRGRCHVVVWFSR
                     GYNCPFCRRYMAQLDRAHDTFITARTEVLQIGPNVLRPARIYFQRQPLKFPFLCDPTK
                     ATYLRYGLRDLGPIEANVNTFRSFAYAYTHGDGINTTRIVAGDTFTEGFLGRLQHHAL
                     TAMQQGMFIVDCDGVIRYANAFKPLDTIPPIEEMLREVEKLRQVS"
     gene            complement(4625..5221)
                     /locus_tag="AC162G_15705"
     CDS             complement(4625..5221)
                     /locus_tag="AC162G_15705"
                     /inference="COORDINATES: protein motif:HMM:NF017496.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR5077301.1"
                     /translation="MAVQAPTQPPAERQWPPPQGQWTYDDYKRLPDDGWRYEVLEGEL
                     LMTAAPSTKHQRTSLNLSSALHQHVTEQELGEVYTAPIDVILPDLASPVQPDILFIAR
                     ERLSIVKEDSIEGPPDLIVEILSPSNWLADRRTKFRVYALAGVREYWIVDTDTRSVEV
                     FVLRGSSFAQVGKFGPGETVRSEVVAGFAISVDDICPA"
     gene            complement(5310..7172)
                     /locus_tag="AC162G_15710"
     CDS             complement(5310..7172)
                     /locus_tag="AC162G_15710"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5077302.1"
                     /translation="MCARLRIPICLVAGVMALTLLAANSRPAEPLPVETPTATIEPTP
                     TLAPIPSTATPAPEPKTLTVCIAEPEPPLLPYVQTSLAGRTIMQAIYDGPIDHLRDGY
                     HPVIVEKLPRLEDGDAQITEAAVKAGDTIVNEDDEIVTYEGPDTTLPQIVATFRLKPS
                     LNWSGGEPLTALDSVFAYQIITDPDVLKDNALAFSLPRDLLDRTATYRALDDLTVEWV
                     GLPGYLDREYFTRFLTPMPKHKLAGRDPVEFLDDADTLPVGWGPYRLVRWQPGDQMIL
                     ERNPFYFRVSEGLPRLDRVVFRFSGFEVIDVQGNVDYSSVVAAVAVGDCDIVSIDPGT
                     NLPLDVWQARVPDVQAHRTPVGVEYLFFNLDRLAGDPRPDFFGDRRVRLAFAHCIDRV
                     GLGVSAAALPADELPWTLSSDKAIVYAYDPDRGRALLAEAGWTDANGDGVADKGGVPF
                     EVTLLTTTSPLRQEVAERIRNDMAACSVRVNPEFLEPGKLFAEGPDSPLYGRQFDLAE
                     FAWLSNDLSVCQTWRSDRIPSDTVGFAGSYQNYSGYRNPDFDAACKAHLRGVMEADRQ
                     RAEEELIALFAEDLPSLPLFWREWHTGARAGVIGFGLDPFAAELWNIEEIDVQP"
     gene            7429..>7674
                     /locus_tag="AC162G_15715"
     CDS             7429..>7674
                     /locus_tag="AC162G_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077303.1"
                     /translation="MSASTKKSFTIVSLSIVAVLLLSACQPSAPTDLLGEIQARGTIR
                     ISTDPNYAPQSSAVPDATRKADTKCASDELTSAELEGF"
BASE COUNT         1413 a         2441 c         2490 g         1330 t
ORIGIN      
        1 tgagcgagcg cgcgcagcgc aagcgggctg cggagctgct cgcgatggtc gggctgggcg
       61 acagactgag ccatctgccc ggccagctct cgggcggcca gcggcagaag gtcgccatcg
      121 cccgcgcgtt ggcgaacaat cccgccatca tcctggccga cgagccgacc ggcaatctcg
      181 acagcgcgag cggcgacgag ctgctgagcg tgttcgagca actgcacagc cagcgcggga
      241 cgacgatcat catcgtcacg cacgatgcgg cggtcgcgcg gcgcacgcac cgcatcctga
      301 tgatgcagga cggccggatc gttcacgaag acatcgtggg cgacgcgtat accgaggatc
      361 tcaaggcgct ggcgcgctcg gcgctgggtc gcgccgtgct gggcggtgcg ccgctagaag
      421 ttctgtcgaa cggcgagcgc gaggtgatga gccgggtgct ggcacgcacc gtcgaacgaa
      481 gattacgata gggttacaaa tcaaacaccg gggccgcatc tgacgtataa tctgacgtaa
      541 tgtcagacct cgaatctcag atcgccgagc ttcgcacctc cattgccgcg ctggaagcgc
      601 agcgcgccgt gctgggcgag gtggtcgtca acgccgcgct cggcccgatg cgcgagaagc
      661 tcgccgcgct cgaagcggcg ctccaatctc cctcgtttca tccgaaggag tccgacgccg
      721 agggcgagcg caaggtcgtc accatcctct tcgccgacat ctccggcttc accgcgctga
      781 gcgagacgct cgacccggag caggttaccg acatcgccaa tgcctgtttc cagtcactga
      841 cggacgccgt gacgaggtac ggcggcacga tcgacaaata tctgggcgat gcggtcatgg
      901 cgcttttcgg cgcgcccgac gcccacgaag acgatcccga acgggcgatt cacgctgcgc
      961 tcgaaatgca gtctgcgctc cggcagacga tctctcaact atcacctgcc accggcgatg
     1021 gttcggcttc gctcaccaca ggcctggcgc tacacattgg catcaacacc ggccccgtcg
     1081 tcgcgggcgc tgtcggcgcg gccggcctgc gcgactacac ggtgatgggc gacgcggtca
     1141 atctcgcctc gcgcctgaag gacgtcgcgc cgatcggcca catttacgtc gggccggaga
     1201 cctatcgaca gactagccgc ctgttcgagt tcgcgccgct cgcgccgatg cgcatcaaag
     1261 gtaagcgcga tccggtgcag atctacgagg tgcgcgggcg agcggcgtcg cccggcacga
     1321 tgcgaggcat cgaagggctg cgctcgcctc tggtcgggcg aggcgacgaa ctcgacctgc
     1381 tgaaggcgcg gctgcgctcg cttccggaag ggaagggcag cgtcgtcgcg ctcactggcg
     1441 aggcgggcct cggcaagagc cgcctgatct ccgagctgca cgtcgccgtg cacactgcca
     1501 acacctaccc cgatcccggg gtacgggcag gccttgcgcc tgcccaacat cgcgccggcg
     1561 agtcggccgt gctgtggctg gaaggtcgct gcacttcatt cacacaatcc gtatcgtaca
     1621 gtctcatcgg gcaggtcttg agcagctact tcggggtgag cggcagcctg gatgcgagcg
     1681 aggcgctgga gcggctgcaa gaggcgctcg atgccgtgct gcccggccag ggcgacgagc
     1741 ttgcgccgta tctcggcacg ctgttaggat ggcaggtagg cgacgagcgc gtccgctatc
     1801 tcgacgccga agcgctgcag cgccagattt tctacagcgt gcggcgcttc gtcggcggcc
     1861 tgtcgacgca aacgccgctc gtgatcgtcc tggacgatct acactggatc gacgaggcgt
     1921 cgctcgacct gctcgaatac gtgctgccgc ttgtcgaaga tcatccgttg atgatggtgt
     1981 gggcctttcg gccgggcaca gagcagacgg gcatcgagca gctcgccaga cggttcgcac
     2041 agaccggatc gcagcatctc tatcaggtcg atctgcgccc gctggccgac gacatgagcc
     2101 aggccctggt gcgcttgctg ctcgcacgca gcgatctacc cgagccgata caggccgcca
     2161 tcgtcgagcg cgccgagggt aatccgttct atctcgaaga agtcatacgc acgctgatcg
     2221 agtccgccct gctgatagaa aagggagggc gttgggtgat cgacccgcag atccccgacg
     2281 tggagttccg catccggtcg gcgctgcccg acacgttgat cggcgtgctg acggcgcgca
     2341 tcgaccggct gcccgagcag cccaagcgtg tgctcggcat cgcgtcggtc gtgggccggg
     2401 cattctcgca tcgcctcatg acggagttga tcggcagccg gacggctgtc gacgaggcgc
     2461 tggccgagct ggagcgcagc gggctggtcg aatccggcga agcgaccccg ctgggcgggg
     2521 cgcaagcagc atggggaccc acttactcct tccggcacgt gctcacgcag gaggcggcgt
     2581 acaacatgct gctgcagcgc cgtcgcgtcc aactgcacgg cgagatcggc gacgcgctgg
     2641 agcgcctgca tagggacgaa gtcgacgagt acgccgtgct gttggcgtat cactacgatc
     2701 gcagccagaa cgatgcgaag gcgatcgcgt acggcctgcg cgccggccgc gctgcgctca
     2761 gagtgtacgc caatcgcgag gcggcgggtt tcttcgcccg ggtcgtgaac cggctcaggg
     2821 aatcgggatc ggaggatgca cagcttgccg aggcgctcga agctctaggc gatgcgcgtt
     2881 cggcgtcggg cgaagtcaac gcggcgatcg ggtactggca ggaagcgctc gtcatgcagt
     2941 cgcgcttcga aggtcgtgaa agcgtcgccc gcctgaatcg caagctcggc aacgcctggt
     3001 tcacacgagg cgaccgggcg cgggccattg agacttaccg gcgcggtatc gacgccgtgg
     3061 gcgatttgcc gcccggcgag acgctggcga gcctatacgc cgagctgggg cgcgtgtact
     3121 tccgggccgg gcaggacgcg caggcgatcg actgggcggc gcgcgcgctc gatctggccg
     3181 aatcggtggg cagcgccgag acggcctcgc tcgcgctcaa cacgctgggc atcgcgcggg
     3241 cgcgcggcgg cgagctggcg cgcggcatcg ccgaggtcga gcgcggcctt cagattgcga
     3301 tcgacaacga tctgcccgtc gccgcctgcc gtgcctacac gaacctgggc atgctatacg
     3361 gcgcggtcga ccacagccgg gcggtgcagt tgtgccagca gggcctggcg cttgcgcgca
     3421 agatcggcga tctatactat caatcgtggc tgcaatcggc gctggcctcg acgtattgct
     3481 cgctcgtcgg aaactgggag cgcggcgtcg ccgctgcgcg cgcctcgatc gatctcgacc
     3541 tgcagatggg acatcgggcg cacctggccg tgccggtcgt gctgctggcg cagatttacc
     3601 agtgccacgg catgctcgaa gagtcgctgc gcgcgtaccg ggaggcgctg gcgctggccg
     3661 aagagagcgg cgagccgcag atgctattcc cgtgctacaa cgggcttggc gcgctgtatc
     3721 tcgaaatcgg cgacgaggaa caggctacga cttatctgga caaggcccag caggtggtga
     3781 gcgaggcggg gtacagcgcg gattcgttgt tcgtgctgcc gttcctggcg tagttagagt
     3841 agggagttag agtcaagacc gttcgaataa gagtgtagtc gagcctttag gcggttgaat
     3901 ccggctaaag cctcgattcc agcatggttc cgaggggaaa cggtggatac gacgaaggac
     3961 gaggtgaaaa ttggctcgcc tgcgccggag atcgagcttg aatcggttca gggaccggcg
     4021 gttcgcctga gcgaattccg tgggcgatgc cacgtcgtgg tgtggttttc gcgcggctac
     4081 aactgcccgt tctgccggcg ctacatggcg caactcgatc gcgcccacga cacgttcatt
     4141 accgcgagga ccgaagtgct ccagatcggg ccgaacgtgc tccgcccggc gcgcatctac
     4201 tttcagcgcc agccgctcaa gttcccgttc ctgtgcgacc cgaccaaagc cacttatctg
     4261 cgctacgggc tgcgtgatct ggggccgatc gaggcgaacg tcaatacgtt cagaagcttc
     4321 gcctacgcct acacgcacgg cgacggcatc aacacgacgc gcatcgtcgc cggcgacacg
     4381 ttcaccgagg gcttcctcgg ccgcctgcag caccacgcgc tcacggcgat gcagcagggc
     4441 atgttcatcg tcgattgcga cggcgtgatc cgttacgcca acgccttcaa gccgctcgac
     4501 accatcccgc cgattgagga aatgctgcgc gaggtagaaa aactgcgaca agtttcctga
     4561 gttccttcag ttccccgtcg gtaaggaatt caaggaactg agggaactga aggaactgga
     4621 ggaactacgc ggggcagatg tcgtccacgc tgatcgcgaa cccggcgacg acttcggagc
     4681 gcactgtctc gcccgggccg aacttgccca cctgcgcgaa gctgctgcct cgcaatacaa
     4741 agacttcaac agagcgtgta tccgtatcca cgatccagta ctctcgcacg ccggccagcg
     4801 catacacgcg gaattttgtc cgccggtcag caagccagtt gctcggagaa agaatttcca
     4861 cgatgagatc gggcgggcct tcgatagagt cttccttgac aatgctcaac cgctcgcggg
     4921 cgatgaacaa aatgtcaggc tggacgggac tggccagatc ggggagaatc acgtcgatcg
     4981 gtgcggtgta gacttcaccc aattcctgct cagttacgtg ctggtgcaaa gccgagctga
     5041 gattcaagct ggttctctga tgcttggtgg atggagccgc cgtcatgagc aattctcctt
     5101 cgagcacttc gtatcgccag ccatcgtccg gcagtcgttt gtaatcgtcg tacgtccact
     5161 ggccctgtgg gggcggccac tggcgctcag ccggcggctg ggttggagct tgcactgcca
     5221 tggcttgttc tcctttccgc gcacgttgcc acgctgcgca gaatgtatca cacctgaccc
     5281 tcaggggcaa gttggcgcgg cagcgtctgt cacggctgaa cgtcgatctc ctcgatgttc
     5341 cacaactccg ccgcgaacgg gtccaacccg aagccgatca ccccggctcg tgcaccggtg
     5401 tgccactcgc gccagaagag cggcagcgag ggcaggtcct cggcgaacag cgcgatcagc
     5461 tcctcctcgg cccgctgtcg atcggcctcc atgacgccgc gcaggtgtgc cttgcacgcg
     5521 gcgtcaaagt cggggttgcg gtagccgcta tagttctggt agcttccggc gaaccccacg
     5581 gtgtcagacg gtatgcgatc cgagcgccac gtctggcaca ccgacagatc gttcgacagc
     5641 caggcaaatt ccgccaggtc gaattgccgg ccatacagcg ggctgtcagg cccttcggca
     5701 aacaacttcc ccggttcgag aaactcagga ttgacgcgca cactgcaagc agccatatcg
     5761 ttgcggatgc gctcggccac ttcctgacgc agcggcgatg tcgtcgtgag cagtgtcacc
     5821 tcgaagggca caccgccttt gtctgcgacg ccgtcgccgt tcgcgtctgt ccaacctgcc
     5881 tcggccagca acgcgcgtcc gcgatcagga tcgtaagcgt agacgatcgc tttgtcggaa
     5941 gagagggtcc acggcagctc atcggcaggc aacgccgcgg cagagacgcc caggcccaca
     6001 cgatcgatgc aatgcgcaaa ggcgagccgc acccgccggt cgccgaagaa atcggggcgg
     6061 ggatcaccgg cgagccgatc caggttgaag aacaggtact ccacgcccac cggcgtgcga
     6121 tgcgcctgga catcagggac gcgtgcctgc cagacatcga gcgggaggtt ggtccccgga
     6181 tcgatggaca cgatatcgca gtcgccaaca gcgacagccg ccactaccga tgagtagtcg
     6241 acgtttcctt gaacatcgat cacctcaaac ccactaaacc tgaaaacgac gcgatccagc
     6301 ctaggcaggc cttccgacac gcggaagtaa aacggattgc gctcgagtat catttgatct
     6361 cccggctgcc aacggacgag ccggtacggt ccccacccca caggcagcgt gtcagcgtca
     6421 tccaggaact cgaccggatc gcggccggcc agcttgtgct ttggcatcgg cgtgaggaag
     6481 cgcgtgaagt attcccggtc gagatagcct ggaaggccaa cccactcgac ggtcaggtca
     6541 tccagggcgc gataagtcgc ggtgcggtcg aggagatcgc gcggcaggct gaaggccaga
     6601 gcattatcct tcagcacatc cggatcggtg atgatctggt aagcaaagac ggaatcgagc
     6661 gcagtcagcg gctcgccgcc cgaccagttc agacttggct tcagacggaa ggtggcgacg
     6721 atctgcggca acgtcgtgtc aggcccctcg tacgtcacga tctcgtcgtc ttcgttcacg
     6781 atcgtgtcgc ctgctttcac ggccgcctct gtgatctgcg cgtcgccgtc ttcgaggcgc
     6841 ggcagtttct ccacgatgac cggatggtag ccgtcgcgca gatggtcgat cgggccgtcg
     6901 tagatcgcct gcatgatcgt ccgcccggcg agcgaggtct ggacgtatgg cagcagcggc
     6961 ggctcaggct cggcgatgca gacggtcagc gtcttcggtt caggtgccgg cgttgccgtc
     7021 gagggaatcg gtgcgagagt gggcgttggc tcaatcgtgg ccgtcggcgt ttcgacggga
     7081 agcggttcgg cgggccggga gttcgcggcg agcagcgtca gtgccatgac gccggccaca
     7141 aggcagatcg gtattcgcag gcgagcgcac attcaggcct ccttttgcgg cctacaatgg
     7201 gcacgcggac gacccacccc aagcgcacaa cataattgta caccgatatt cgcgcccagt
     7261 ccgtcagttg acccccgctt cggttatgct agaatgaata tattccgtct gtccgccacc
     7321 ggagatgcgc atcatcttca tacatgaagg aggtgattga acacttcgtc acgaaacacg
     7381 atcaggctac ccaccgtcgc atgcacattc tctcaggagg agatacacat gtccgcttca
     7441 acgaagaagt cgttcaccat cgtcagtctg tcgatcgtcg ccgtgctgct cttgagcgcg
     7501 tgtcagccca gcgcgccaac cgacctgctc ggcgagatcc aagcacgcgg cacgatccgc
     7561 atctcgaccg atccgaacta tgctccgcag tcgtcagcgg tccccgacgc gacacgcaag
     7621 gccgacacga agtgcgcctc cgacgaactg acatccgctg agttggaggg cttc
//
LOCUS       DBNHXB010000389         3281 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3633897, whole genome shotgun sequence.
ACCESSION   DBNHXB010000389 DBNHXB010000000
VERSION     DBNHXB010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3633897"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1801
                     /locus_tag="AC162G_15720"
     CDS             <1..1801
                     /locus_tag="AC162G_15720"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR5077304.1"
                     /translation="GGIVRVTGSGLGCPDWPTCHGYLIPPPGREPWIEFSHRLVAGVV
                     SVLVAAIVVAAWRSYRSDRAILRPALAAAGALIVQILLGAITVVLELPPLIVWIHLGA
                     ALLVFACLIVVATNARMAGGWSSGQVVGWSGGRMEARLRSRIVALALASAISILVLTL
                     LGATVVNTGASLACPDWPLCRSAIVPPLSDPLVVVHFTHRFAALAAGIPVLLFAVEAR
                     RVRAQAPRVSRGALILLGLFVAQVVVGGLTALWSLPLILRALHLAVAAAAWGVAVALA
                     VVLARSRPVASEAPAQERQARAEPASNAGAAHPSTGGSAFRKYLYLAKPWIIALLLTT
                     TLTSMLIAGRGSVSLPLAVFTLIGGALASAGANAINCYVDRDIDGLMSRTKNRPIPRH
                     QLDPRRALAFGLVASSASFAILLVFVNLLAATLAVFGIIYYVFVYTLWLKRSTPHNIV
                     IGGIAGAVPPLVGWAAVTGRVDLLALYLFLIVFYWTPPHTWALAILVRGDYERANVPM
                     LPVVRGEAETRRHIWLYSLQMVAVTLLLFAFRMLGWVYLALALVLNGLFLWYAYRLTR
                     EMDKPAARRLYKFSQLYLALLFVAMVVDASALS"
     gene            1832..2278
                     /locus_tag="AC162G_15725"
     CDS             1832..2278
                     /locus_tag="AC162G_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077305.1"
                     /translation="MLTELVSQLQMQSVLTAGAALSGVQQQASDDVVRTVTSILLISA
                     CLLPILMAVFGAVVMRFLGWFTGPREAQADEVVYAEPALPSGVHLPASTIWPAVLAFG
                     LMGLLFAIPLEGWLEAIAALVGLVLTAVGLIGWIVLAVREARAHGS"
     gene            2293..2730
                     /locus_tag="AC162G_15730"
     CDS             2293..2730
                     /locus_tag="AC162G_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077306.1"
                     /translation="MSKNVLAGIAILLASGFATWLFLPMVSESLAETPPPPEPVIVQI
                     GPIQVNMMLFVLFFAAGVPFMGLVMAVVLRWLGKFVPARASTPAPAPTSRKSQPSAPA
                     AQATAQPDLPLVQKAILMLVMLGAIGFIVYLLSLVLPPGFTAF"
     gene            3092..>3281
                     /locus_tag="AC162G_15735"
     CDS             3092..>3281
                     /locus_tag="AC162G_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077307.1"
                     /translation="MRADGSTTYAYDFANRLISTTLGGATTQFAYNRDGIRMRLVEAG
                     TLTTYTHDYASPLPVVLQA"
BASE COUNT          431 a         1057 c         1100 g          693 t
ORIGIN      
        1 cggcggcatt gtgcgcgtca cggggtcggg gctgggctgc ccggactggc cgacctgtca
       61 cggctacctt atccctccgc ccgggcgcga gccgtggatc gagttctctc accggctggt
      121 ggcgggcgtg gtgagtgtgc tcgtcgcggc catcgtcgtg gctgcgtggc gttcgtatcg
      181 ctccgatcgg gccatcctgc gaccggcgct ggctgccgcg ggtgcgctga tcgtgcaaat
      241 tcttctgggc gccatcaccg tcgtcctgga gctgcctccg ttgatcgtgt ggattcacct
      301 tggcgcggcg cttctcgtgt tcgcctgcct gatcgtcgtc gccacgaacg cgcgcatggc
      361 gggcgggtgg tcaagtggtc aagtggtcgg gtggtcgggt ggtcggatgg aggcgcggct
      421 ccgctctcgg attgttgcgc tcgcgctggc ctccgcgatt tcgatcttgg tcttgacgct
      481 gctgggcgcg accgtggtga ataccggggc gtcgctggcc tgccccgact ggccgttgtg
      541 ccggagcgcg atcgtcccgc cgttgagcga cccgctggtc gtcgtgcact tcacgcatcg
      601 cttcgccgcg cttgcggcgg gcattccggt gttgctcttc gccgtcgaag cgcggcgtgt
      661 tcgcgcgcag gcgcctcggg tgtcacgcgg cgcgctgatt ctgctcggcc tgttcgtggc
      721 gcaggtcgtc gtgggcggct tgacggcgct gtggtcgctg ccgctcatcc tgcgcgcctt
      781 gcatctggcc gtggcggcgg cggcgtgggg cgtagccgtg gcgctggccg tcgtcctggc
      841 gcgcagccgg ccggtcgcgt ccgaagcgcc cgcgcaggag cgccaggcgc gggcggagcc
      901 ggcgtcgaat gcgggggctg cccatccgtc gacgggcgga tcggctttca ggaaatacct
      961 ttacctggcc aagccgtgga tcatcgcctt gctgctgacg acgacgttga cctcgatgct
     1021 gatcgccggg cggggcagcg tgtcgctgcc gctcgccgtc ttcacgctga tcggcggcgc
     1081 gctggcctcg gcgggcgcga acgcgatcaa ctgctacgtc gatcgtgaca tcgacggcct
     1141 gatgagccgc acgaagaatc gcccgatccc gcgccatcaa ctcgatccgc gccgggcgct
     1201 cgcgtttgga ttggtcgcca gcagcgcgtc cttcgccatc ttgctggtgt tcgtcaatct
     1261 gctggcggcg accctggccg tgttcgggat catctactac gtgttcgtgt acacgctgtg
     1321 gctgaagcgc tcgacgccgc acaacatcgt catcggcggc atcgccggcg ccgtcccgcc
     1381 cctggtgggc tgggcggcgg tgacaggccg cgtcgatctg ctggcgctct atctgttcct
     1441 gatcgtgttc tactggacgc cgccgcacac ctgggcgctg gcgattctcg tgcgcggcga
     1501 ctacgagaga gctaacgtgc ccatgctgcc cgtcgtgcgc ggcgaggctg agacgcgccg
     1561 ccacatctgg ctctattcgc tgcagatggt ggcggtcacg ctgctgctgt tcgccttccg
     1621 catgctgggc tgggtctatc tggcgctggc gctcgtgctg aacgggttgt tcctgtggta
     1681 cgcttaccgc ctgacgcgcg agatggacaa gccggccgcg cgtcgcttgt ataagttctc
     1741 gcagttgtat ctggcgctgc tgtttgtggc catggtcgtc gatgccagcg ccctgtcgta
     1801 aatcaattat cgtgttggag aaatcgcgcg tatgctgacg gaactcgttt cgcaactaca
     1861 gatgcagagt gtgctgacgg ccggcgcggc gctgtcgggc gtgcagcaac aggcgagcga
     1921 cgatgtcgtc aggacggtca cgtccatcct gctgattagc gcatgtctgc tgccgatctt
     1981 gatggcggtc tttggcgcag tcgtgatgcg ctttctgggc tggtttaccg gcccgcgcga
     2041 ggcccaggcg gacgaagtgg tctacgccga gccggcgctc ccgtcgggcg tccatctgcc
     2101 tgcttcgacg atctggccgg cggtactggc gttcgggctg atgggcctgc tgttcgcgat
     2161 tccgctggaa ggctggctgg aagcgattgc tgccctggtg ggcctcgtcc tgacggcggt
     2221 gggtctgatc ggctggatcg tgctcgcggt ccgagaagcc cgcgcacacg gcagttgatg
     2281 cggaggatcg gcatgtccaa gaacgtgctt gcgggtattg ccattctgct ggcatccgga
     2341 ttcgcgacct ggctgttctt gcctatggtc agcgagtcgc tggctgagac acccccgcca
     2401 cccgagccgg tcatcgtcca gatcgggccg attcaggtca acatgatgtt attcgtcctg
     2461 tttttcgcag ccggcgtgcc gttcatgggg ctggtgatgg ctgtcgtctt gcgctggcta
     2521 ggcaaattcg tgcccgccag ggcatcgacg cctgcgcctg ctcccacctc acgaaagtcg
     2581 cagccgtccg cgcctgccgc gcaggccacc gcgcagccgg atctgccact ggttcagaag
     2641 gcaatactta tgctggtgat gcttggggcg atcggcttta tcgtgtatct cctctcgctg
     2701 gtgctgcctc ccggcttcac tgcgttttag agcgcttcca ggatgctttc cgcccccggc
     2761 cggtgcgagc cgaggggctg aaaccgatcc ggatgggcgc gggtctccta accgcgccct
     2821 ttttctgcac gccacgctgc tgggtacatc cgctgcacct gcgacccgct gtatcgcctg
     2881 accggcgcga attactccgg cgcgtacacc tacaccttcg cctatggcta cgatgccgtg
     2941 ggcaaccgca ccgtccagac ccggacaatc acctcaacca tagtgacgac gtacatcctt
     3001 cgacttcgct caggacaggc tctgcgacgc cgcgaaccgc ctcgccacgc agaacgggca
     3061 gagcattttc acctgggata acaatggcaa tttgcgggcc gacggcagca cgacatatgc
     3121 ctacgacttc gccaaccggc tgatcagcac gaccctgggt ggcgcgacca ctcaatttgc
     3181 gtacaatcga gatggcatcc gcatgcgcct cgtcgaggcg ggaaccttaa caacctatac
     3241 gcacgactac gcatcaccgc tgccggttgt attgcaggcg a
//
LOCUS       DBNHXB010000390        14489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3129163, whole genome shotgun sequence.
ACCESSION   DBNHXB010000390 DBNHXB010000000
VERSION     DBNHXB010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 14489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14489
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3129163"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="AC162G_15740"
     CDS             <1..414
                     /locus_tag="AC162G_15740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HIR5077308.1"
                     /translation="IVLDTVVFVRALINPKSRSGRLLFEYSDRYKIIISKPTAQELLE
                     VIHRPELTAKYHGLARISLRKVIDLLSKAEAVDIGTPPSVVRDPKDDIFIATALAGRI
                     DYLVSEDKDLLALGNTAGVPVINTQIFIELLESES"
     gene            complement(433..1956)
                     /locus_tag="AC162G_15745"
     CDS             complement(433..1956)
                     /locus_tag="AC162G_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR5077309.1"
                     /translation="MAQILFGQSYYLRFDPKLYMAMQPYPPLGTLYAASYMRARGWDV
                     ALFDAMLAASEQEWAAALDRHKPQVAVLFEDNFNYLSKMCLLRMREAAFTMIEMAKAR
                     GCIVILCGADATDNSLKYFARGADFALIGEGEETLGELMDALRTHPERSAAQSKDAFA
                     SILGLAWPDGKGGEHRNPRRPDITRLDDLPFPAWDLVDIARYQKVWRQRHGYTSMNMV
                     TTRGCPYHCNWCAKPIWGQRYNSRSPENVVAEMKWLKENYRPDHIGFADDIFGLKPGW
                     IERFGRLAEEQDAVIPFKCLLRADLVKPSVVEGLKSAGCRSVWMGAESGSQKILDGME
                     KGTTVSQIAEARRLLGNAGIQVAFFLQFGYPGETRADVEATLKLVRETMPDDIGISVS
                     YPLPGTKFFERVKSQLGEKQNWFDSDDLAMLYQGPFPQAFYRVLHRAVHAEFRMRRAW
                     RVIRCVAPHPRRWRLRHARLLASIPYHWLGWQTNRLRLNRFKPNAGTVQQTMIPESH"
     gene            complement(1974..2903)
                     /locus_tag="AC162G_15750"
     CDS             complement(1974..2903)
                     /locus_tag="AC162G_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077310.1"
                     /translation="MQAPGDAASRREIESAILETVLYSDLFDYPLTYAEIAHYLIGVP
                     ADADMVRTLLAAPSYLDGHLRKCDSYVFAHPREAIVDRRRARRGSSARLWMRARRFVR
                     VMAILPFVRMVGITGALAMDNSAPGDDIDVMIVAASRRAWTARLFVVALVYAGKLFGH
                     TLCPNYVITEDALELETRDLFAAHEFAQMVPVYGFAVYDRMRQANRWVYDYLPNAYAP
                     LRREREVRPGILGRAIKRAGERLLTGRLGEALEEWEMRRKQRKFASRMTDRSRAILDR
                     DHVKGHFEDYGVSVMELYAERLAQFPIPDAEDA"
     gene            3043..3537
                     /locus_tag="AC162G_15755"
     CDS             3043..3537
                     /locus_tag="AC162G_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077311.1"
                     /translation="MWRALSGRQRGVLIGLGLANLILLGAIAALALSPAPGTTPIVTP
                     APLHTVNACQAEAARALAELNVAGIVAIRDDGAIDFTMSGDDPADAWVAFAVSAELPK
                     LDCGPYDPIRVDTPDPSRIANRRLVVEASWLDVQAWSQGRIDDEVLSERMTRTSYMRP
                     GLSP"
     gene            complement(3526..3735)
                     /locus_tag="AC162G_15760"
     CDS             complement(3526..3735)
                     /locus_tag="AC162G_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077312.1"
                     /translation="MSDDRWIAWLVAIVMALLVMALFLRRSAPLGRSRGRYRLLEWAT
                     LIATALAAGAMMGLVTYLVVSGLGR"
     gene            4016..5167
                     /locus_tag="AC162G_15765"
     CDS             4016..5167
                     /locus_tag="AC162G_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR5077313.1"
                     /translation="MIISRQRLEQIEQETLAPYGMRSRNTRGRIHPEEEHPYRTAYQR
                     DRDRIVHTTAFRRLERKTQVFVVTEGDYYRTRLTHTLEVAQIGRTLARALGANEDLTE
                     AICLVHDIGHPPFGHSGEWTLNQLMKERGGFDHNKQALRIVEALERRYPDWPGLNLTY
                     EVREGIVKHETEYDLSSAESYEPDQRGLLEAQIANAADEIAYNAHDADDGLRAGLFAA
                     RDLEGLALWEMVKDSVGWDGKGFDDLWRHRLIRRLIGMEVTDVVRATSKQLQADGVKS
                     VDDLRRLDHNVVRFSDEMRVRNREHKDFLFQRMYQHFRVLRMQAKAERLLQQLFEAYV
                     GEPRQMPESTQARIERVGLHRAVCDYIAGMTDSFALDEHQKLFDPAVRV"
     gene            complement(5270..6337)
                     /locus_tag="AC162G_15770"
     CDS             complement(5270..6337)
                     /locus_tag="AC162G_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077314.1"
                     /translation="MASLGTQLRASWPMVKAVRRELLIVGVVFLIGVIVGWFVLGWWL
                     APVEWTDAAPVDLQPTYRDAHVASVAAAYNTGAFTSNEVESLGKGERWSYAGFIAELN
                     RLAADASPQMKTAYEKLALDLAPLAAQEQAAQPPPAAQPSPLVWLLLILALAAVAFFT
                     IMLVRRGGRAAPAGAVGAARAPGGVPMAPGAVAVWPDEAQLPLRQFDLTYVIGDDRFD
                     LSNAIETSSGMFLGECGMGISETIGTPDPSKVTAFEVWLFDKNDIRTVTSVLMSEHAY
                     NDPALKAKLAAKGDLVKADPGTLVTLETATLRVRAKVAEVEYGAGALPEKSFFNRLHV
                     VMAAWPIGDSGITQPADAVPV"
     gene            complement(6458..7528)
                     /locus_tag="AC162G_15775"
     CDS             complement(6458..7528)
                     /locus_tag="AC162G_15775"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR5077315.1"
                     /translation="MTDRVAQVRGRLRELEIDALLVTQAENRRYLSGFTGSAGVLLIT
                     LDRSILATDSRYWEQAAKQAPTFELVQIKTRLEDHLADILGKAGYPKRVGFESAAVSV
                     DQFSTWSNMNGGVVWVATKNAVEPIRAVKDESELAAIRKAAAVTDAGFDFLCGVLKPG
                     MTEREAAWELEAFLRTHGAEALAFDVIVASGPDSAMAHHHPGDRAIQAGEPILIDFGA
                     KVDGYCADLTRTVSLGRADEKYDEVYEIVRRAQQVALDGMRAGMPGVQADALAREAIV
                     AAGYGDFFGHGLGHGVGLAVHEAPRAGRVDAEAPLPVGATLTVEPGVYLAGWGGVRIE
                     DLVVVRENGVEALSRAHKRSII"
     gene            complement(7587..10022)
                     /gene="topA"
                     /locus_tag="AC162G_15780"
     CDS             complement(7587..10022)
                     /gene="topA"
                     /locus_tag="AC162G_15780"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR5077316.1"
                     /translation="MEAYCVKCKTKREIVNPQPVFTAGGTPATKGACPECGTALFRMG
                     WTPAHEGIPRPEATARSPRNGKRNRAPAERRGALVIVESPAKAKTVGRFLGRKYTVKA
                     SVGHVRDLLRSQLSVDVDNGFAPKYRVPNEKKAVVKELAELGQRAEEVYLATDPDREG
                     EAIAWHLVESADFERERVKRVVFHEITEHAIGEAFAHPRSINMELVNAQQARRILDRL
                     VGYKLSPLLWEKVRSRLSAGRVQSVALRLIVEREREIQAFVPVEYWSIHAELAKHLEP
                     QAHAPKKRPSFLAKLVKIKGQDVDLKNEADTQGIVAELEKADYVVAGVKKGERRRRPS
                     PPFTTSTLQQEASRKLGYPARRTMALAQQLYEGIALGDEGSVGLITYMRTDSTSVAAE
                     AQQEARAFVSERYGAEYLPDEPPQYKTRSKNAQEAHEAIRPTSVRRTPEAVKPFLDAA
                     QLKLYSLIWKRFLASQMNAAVYDTVSVDVAAGEVGELSDVIRDLGDASVNGVAGRFPY
                     LFRSTGSTLKFAGYLVVYEEARDEDVAPEEDEGARIPELSADELLDLLALLPDQHFTE
                     PPPRYTEASLVKALEENGIGRPSTYAPILSTIQGRGYVERREKRLWPTDVGVIVNDML
                     VEYFSDVIDVGFTARMEEELDEIADGEKDWAEVLREFYEPFEKDVEYALHAIEKVEVN
                     EEIGEACPTCGKPLIVRWGRFGKFIGCSDFPTCRYTRPFLIKIGVKCPLDGGDLVEKK
                     TRRGRIFYGCANYKNDDPDSCQFTSWNRPLPQPCPNCGGLLTVKGKDQARCLRCENEY
                     SLNELPEMEPVAD"
     gene            complement(10070..11152)
                     /locus_tag="AC162G_15785"
     CDS             complement(10070..11152)
                     /locus_tag="AC162G_15785"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004475 - mannose-1-phosphate
                     guanylyltransferase (GTP) activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HIR5077317.1"
                     /translation="MHDNFYALIMAGGTGTRLWPLSRESRPKQALPLLGDRTMFQIAL
                     DRLAPLFTPDRMLVVAGSNHVDLLKAQGSGIPGENFIVEPMGRGTAPAIALGAIHLRR
                     KHPDAMMAVLTADHFIADEAGFRRALLAAAKVAERGHLVTLGVTPDYPATGYGYIERG
                     DVVESVENFLAYQVMSFREKPSAAAAERFLADGRHSWNSGMFIWRVDRFLAELARTMP
                     EFSAQLDEIDRVLGTPDEASTLANVWPRVASQTVDYGVMEKARDVVVIPIDIGWSDIG
                     SWSSLLDVLPGDADGNVCVHAEHLNAGSRHVLVHGRGRIVATIGLEDVIIVDTEDVVL
                     VCRKDQAEAVKRMVDRLKDEGRTDIL"
     gene            complement(11249..12361)
                     /gene="dprA"
                     /locus_tag="AC162G_15790"
     CDS             complement(11249..12361)
                     /gene="dprA"
                     /locus_tag="AC162G_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR5077318.1"
                     /translation="MDDLRYWVAFNRTPGVGTAKIRALVDHFGNLQAAWHAGEAEWKA
                     AGLDRRAIDSLRAVRAKMDLDAEFARVSACGARLVTWSDPDYPPLLQSIPDAPPLLYV
                     KGELTPADRQWAVSIVGTRRATAYGRQAAERLASDLARSGVTIVSGLARGIDAAAHWA
                     ALRAGGRTIGVMACGIDLVYPAEHARLAAQIAERGALLTETPIGSPPEAGNFPARNRI
                     ISGLSLAAVVVEAGEQSGALITADRALEQGREVFAVPGSIFSRTSAGANRLIQEGATL
                     VRSAEDVLEALNLRMVAQQAEAQAVLTSDPVETAMLKAISHEPTHIDDIVRSMNLSVS
                     QVSSVLALMELKGLVRQVGGMNYVRAREGQAEYVVD"
     gene            complement(12447..12989)
                     /gene="rimM"
                     /locus_tag="AC162G_15795"
     CDS             complement(12447..12989)
                     /gene="rimM"
                     /locus_tag="AC162G_15795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR5077319.1"
                     /translation="MTATRGSGGRAQPEPEFLEVARVLRPWGVTGEVKVKILSSHPED
                     IAPGRQVYVGEDRRACEIQGARAQGDYLIVKLAGYSTPEQAEALRGLHLYIDRGSASP
                     LRPREYYRHQIIGLAVVTLDGVDLGKVEEILETGAHDVYVVHGPRGEVLLPARVEVIR
                     DIDLASGVMRVSLLPGLLPE"
     gene            complement(13013..13240)
                     /locus_tag="AC162G_15800"
     CDS             complement(13013..13240)
                     /locus_tag="AC162G_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR5077320.1"
                     /translation="MRDLLEYVVRSLVDDPSRVSVKESRSGNTVIFELHVAPDDAGRV
                     IGKNGRVANSIRSLLRVAAAKEGKRVQLDIL"
     gene            complement(13389..13655)
                     /gene="rpsP"
                     /locus_tag="AC162G_15805"
                     /pseudo
     CDS             complement(13389..13655)
                     /gene="rpsP"
                     /locus_tag="AC162G_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181719.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            complement(13776..>14489)
                     /locus_tag="AC162G_15810"
     CDS             complement(13776..>14489)
                     /locus_tag="AC162G_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR5077321.1"
                     /translation="IKKLAGPREVMLVADAMTGQDAVRTAEEFHRRVGLTGLILTKAD
                     GDARGGAAISIRAVTGVPIKFLGLGEKADALEPFHPDRMATRILGMGDMLTLIEKAEE
                     AFDKAEAEKAAEKFITAQFDFEDFLEQMQQMKKLGPFAQILDMLPGMGQIKAQIDPAL
                     AETQLKKTEAIIHSMTVAERRNPDVLNGSRKRRIARGSGTTVQDVNQLVRQFREMQKM
                     MRQFNSGRGKGLLKMLGGR"
BASE COUNT         2578 a         4835 c         4372 g         2704 t
ORIGIN      
        1 atcgttcttg acacggtggt ctttgtgcgt gcgttgatca atcccaaaag ccgtagcggt
       61 cgactgctat tcgagtattc agaccggtat aagatcatca tctccaaacc gaccgcccag
      121 gagcttctgg aagtgattca tcgaccagag cttacggcca aataccacgg tctggccagg
      181 atcagtctgc gtaaggtgat tgacttactg tccaaagccg aagccgtcga catcggtact
      241 cctccatctg tcgtccggga tccaaaggac gacattttta tcgcaacggc tctggcaggc
      301 cgtatcgact atctggtcag cgaggacaag gatctgcttg ccctgggaaa cacggcgggc
      361 gtcccggtta tcaatacgca gattttcatc gagctgttag agtcggaaag ctagattcta
      421 ctgtgcggcg catcaatggc tttccgggat catcgtctgc tgcacagtgc cggcattcgg
      481 cttgaatcga ttgagacgca agcgattggt ctgccagccc agccagtggt aggggatcga
      541 agccagcagc cgcgcgtggc gaagccgcca tcgacgaggg tgcggcgcca cgcaccggat
      601 aacgcgccac gcccgtcgca tgcggaactc ggcatgaacg gcgcgatgca gcacgcgata
      661 gaacgcttgc gggaacggcc cctggtagag catggccaga tcgtcgctgt cgaaccagtt
      721 ttgcttctcg cccagttgtg atttgacacg ctcgaaaaac ttagtgcccg gcagtggata
      781 cgataccgag atgccgatgt cgtccggcat tgtctcgcgc accagcttca acgttgcctc
      841 cacgtcggcg cgtgtctcgc ctgggtaacc aaactgcaag aagaatgcga cttggatgcc
      901 ggcgttcccc agcaggcggc gcgcctcggc aatttgggag acggtcgtac ctttctccat
      961 cccatcgagt atcttctgcg agccggactc ggcgcccatc cacacgctcc ggcaccccgc
     1021 tgacttaagc ccctccacaa ccgatggttt gacgagatcc gcgcgcaaca ggcacttgaa
     1081 ggggatcacg gcatcctgct cttcggcgag ccggccgaac cgctcaatcc agccgggttt
     1141 cagtccgaat atgtcgtcgg cgaacccgat gtggtcgggg cgatagtttt ccttcagcca
     1201 cttcatctcg gcgacgacgt tctcgggcga gcgcgagttg tagcgctggc cccatatggg
     1261 tttggcgcac cagttgcaat ggtaagggca gccgcgcgtc gtgaccatgt tcatcgaggt
     1321 gtagccgtgt cgttggcgcc agaccttctg gtagcgcgcg atgtcgacga ggtcccaggc
     1381 cgggaacggc agatcgtcga gccgggtgat gtctggccgg cgcgggttgc gatgctcgcc
     1441 gcccttcccg tcgggccagg ccaagccgag tatagaagcg aacgcatcct tcgactgcgc
     1501 cgcgctccgc tcaggatgcg tccgcaacgc atccatcaac tctcccagcg tctcctcgcc
     1561 ctcgccaatc agcgcgaagt ctgcgccgcg cgcgaagtac ttgagtgagt tatcggttgc
     1621 atccgcgccg cacagaatca cgatgcagcc gcgcgccttg gccatctcga tcatcgtgaa
     1681 ggcagcttcg cgcatgcgca gcaggcacat tttgctcagg tagttgaaat tgtcctcgaa
     1741 gagcaccgcc acctgtggct tgtggcgatc gagcgcggcc gcccactcct gctcggacgc
     1801 ggcgagcatc gcgtcgaaga gcgccacgtc ccaaccgcgc gcgcgcatgt acgacgcagc
     1861 atacagcgtg ccgagcggcg gatacggctg catcgccatg tagagcttgg gatcgaaacg
     1921 caaataatag gattggccga atagaatctg cgccatggct ctctcccgga cgttcaggcg
     1981 tcttcggcat ccggtatggg gaattgggcc aggcgctcgg cgtacaattc catcacggac
     2041 acgccgtaat cctcaaagtg acccttgacg tgatcgcgat cgaggatggc gcgagaccgg
     2101 tccgtcatcc tcgatgcgaa tttgcgctgc ttgcgccgca tctcccattc ttcgagcgct
     2161 tcgccgagtc gcccggtgag gagtcgctcg ccggctcgct tgatggcgcg ccccaggatt
     2221 ccaggcctga cctcgcgctc cctacgcagc ggcgcgtagg cgttcggcag gtaatcatag
     2281 acccatcgat tggcctggcg cattcgatcg taaacggcga aaccgtacac cggcaccatc
     2341 tgcgcgaatt cgtgcgcggc gaagagatcg cgcgtctcca attcgagcgc atcctcggtg
     2401 atgacgtagt tagggcacaa cgtgtgaccg aagagcttcc ccgcatagac cagtgcgacg
     2461 acaaacaggc gcgctgtcca cgcgcggcgg cttgcggcca cgatcatgac gtcgatgtcg
     2521 tcgccgggag ccgagttgtc catcgccagc gcgccggtaa tgcccaccat gcgcacgaac
     2581 ggcaagatcg ccatcacgcg cacgaagcgc cgagcgcgca tccacagccg cgccgaagac
     2641 ccgcggcgcg cgcgccggcg atcgacgatc gcttcacgtg gatgcgcgaa gacatagcta
     2701 tcgcacttcc ggaggtgccc gtcgaggtaa ctcggcgcgg cgagtaatgt ccgcaccatg
     2761 tccgcatctg ccggaacgcc gatcaggtag tgtgctatct cggcatacgt caagggatag
     2821 tcaaacagat cggagtacaa gacggtttcg agtatagcgg attcgatctc gcggcggctt
     2881 gcggcatcgc ctggcgcttg cacggctccc cctccctggt tggcgcctat aagcgcgaaa
     2941 acagaatcag atggtcaatt gtcggatcgc acaaagatac gcgctggatg cccgcctggt
     3001 ttgccgcttg gccagcctcg ccgcgcgtgc taagatgtgg ctatgtggcg agcactttct
     3061 ggccgccagc gcggcgtgtt gatcgggctt ggattggcga acctgatcct gctgggagcg
     3121 atcgccgcgc tggcgctgtc accggcacct gggacaaccc caatcgtcac tcccgcgccg
     3181 ctgcatacgg tgaacgcctg ccaggctgag gctgctcgtg cactggctga actaaacgtc
     3241 gccgggatcg tcgcgattcg cgacgatggg gcgatcgatt tcaccatgag cggtgacgat
     3301 ccggctgatg cctgggttgc cttcgcggtc tcggccgagc tgccgaaact cgactgcggc
     3361 ccctatgatc ccattcgcgt cgacacgccc gatccgtcgc gcatcgccaa ccggcggctc
     3421 gtcgtcgagg catcctggct cgacgtgcaa gcgtggtcgc agggccgcat cgacgacgaa
     3481 gtgctgagcg agcgcatgac gagaacatcg tacatgcgcc ccggcttatc tccctagacc
     3541 cgacaccacg aggtaagtaa ccaaccccat catcgcgccg gccgccagag ccgtcgcgat
     3601 cagcgtagcc cactcgagca accggtatcg acccctggag cgaccgagcg gcgccgatcg
     3661 tcgcagaaac agcgccatga ccagcagggc catcacgatg gcgaccagcc acgcgatcca
     3721 tcggtcgtcg ctcacgggtc gctgcagtcc cgcgtaaagc cgcagttgcg gcacacgatc
     3781 ttgcagtgcc gttcttcggt aggagcgcca cacagccagc actcgccgtc gtcgcccgcc
     3841 cgatctgcga gttgcgcccg tttcggatgg gtaccgtcca tgcacggaat agtaacacac
     3901 attcgagccg gtaggcaagc aggcgtgttt gatttgctaa cccactcgca ctatacttca
     3961 ccaatgtcac atttgcgctc aagccgccgc tcccggcggc gggggagatc gggccatgat
     4021 catttcacgt caacgtctcg aacaaatcga gcaggaaaca ctcgcgccgt acggcatgcg
     4081 cagccgcaac acgcgcgggc ggatccatcc tgaagaggaa catccttacc gcaccgctta
     4141 tcagcgcgat cgagatcgca tcgtccacac gaccgccttt cgacgtctgg aacgcaagac
     4201 gcaagtgttc gtcgtcaccg agggcgatta ctaccgcacg cgcctgaccc ataccctcga
     4261 agtagcgcag atcgggcgaa cgctggcgcg cgcgctcggc gcgaacgagg atctcactga
     4321 ggccatctgc ctggtgcacg acatcggtca cccacccttc ggccattcgg gcgagtggac
     4381 gctcaatcaa ctgatgaaag agcgcggggg cttcgatcac aacaagcagg cgctgcgcat
     4441 cgtcgaagcg cttgaacggc gctaccccga ctggcctggc ctcaatctca cgtacgaggt
     4501 gcgcgagggc atcgtcaagc acgagaccga atacgaccta tcgtccgccg aaagttacga
     4561 gccggatcag cgcgggctgc tggaggcgca gatcgcgaac gccgccgacg agattgcgta
     4621 taacgcgcac gacgccgacg acgggctgcg cgccggtctg ttcgccgcgc gcgacctgga
     4681 ggggttggcg ctgtgggaga tggtgaaaga cagcgtggga tgggacggaa agggcttcga
     4741 cgatctatgg cggcaccggc tcatccggcg gctgatcggc atggaagtga ccgacgtcgt
     4801 gagagccacc tcgaagcagt tgcaggccga tggtgtgaaa tccgtcgatg acctgcgccg
     4861 gctggatcac aacgtcgtcc gcttcagtga cgagatgcgc gtgcgtaacc gcgaacataa
     4921 ggacttcctc tttcagcgca tgtaccagca ttttcgcgtg ctacgcatgc aagccaaagc
     4981 cgagcggcta ttgcagcagt tgttcgaggc atacgtcgga gagccacgcc agatgcccga
     5041 atcgacgcag gcgcgtatcg agcgcgtcgg gctgcaccgc gccgtgtgcg actatattgc
     5101 cggcatgacg gactccttcg ccctggatga gcaccagaag ctgttcgacc cggcggtgcg
     5161 tgtgtagggt cagacgccgc ggccggcaaa cacaaaacac gacacccggc ttcgacgtgg
     5221 cgaagccggg tgtctgactt tcccgttctc ttcgcgcaag cgcggcaact cacaccggca
     5281 ctgcatccgc aggctgggta ataccactgt cgccgatggg ccaggccgcc atcacgacgt
     5341 gcaagcggtt gaagaagctc ttttcgggta gcgcgccggc gccgtactcg acctcggcca
     5401 ctttcgcccg cacgcgcagc gtcgcggttt cgagcgtgac cagcgtgccc ggatccgcct
     5461 tcaccaggtc gcccttcgcc gccagcttgg ctttcaaggc cgggtcgttg taggcgtgct
     5521 cgctcatcag cacgcttgtg accgtgcgaa tgtcgttttt gtcgaagagc cagacctcga
     5581 aggcggtcac tttgcttgga tcgggtgtgc cgatcgtctc gctgattccc atgccgcact
     5641 cacccagaaa cattccgctc gacgtctcta tcgcgttaga caggtcgaaa cggtcgtcgc
     5701 cgataacgta ggtcaggtcg aattgccgca gtggcagttg cgcttcgtcc ggccatacgg
     5761 ccactgcgcc cggcgccatc ggcacgccgc ccggtgcgcg tgccgcgcca acagctccgg
     5821 ccggcgcggc ccggccgccc cggcgtacca gcatgatggt gaagaacgcg acagcagcca
     5881 gggctagaat cagcaagagc cataccagcg gcgacggctg tgctgctggg ggcggctgtg
     5941 cggcctgttc ctgcgccgcg agcggtgcga ggtccagagc cagtttctcg taggcggttt
     6001 tcatttgagg gctggcgtct gcggccaggc ggtttaactc ggctatgaag ccggcgtacg
     6061 accagcgctc gcccttaccg aggctctcca cttcattcga ggtgaaggca cctgtattgt
     6121 aggccgccgc gacgctcgcg acgtgagcgt cacgataggt cggctgcaga tcgaccggcg
     6181 cggcgtccgt ccactcgacc ggcgcaagcc accagcccag cacaaaccag ccgacgatca
     6241 caccgatcag aaagacgaca ccgacgatta gaagctcccg tcgcaccgct tttaccatcg
     6301 gccaggatgc tctgagttga gtgcccaagc tagccatttt gcccccttag ggcctatcca
     6361 aaacaccagc agaagcggct cccatgccgt cggatggctg agcgcgggag cctggcccgc
     6421 cagttttcgg ataggcacgt atgtgcacgt gccagcctca aataatcgat cgtttgtgag
     6481 cgcggctcag cgcctcgacc ccattctcgc gcacgacgac gaggtcctca atgcgcacgc
     6541 cgccccaccc cgctaagtat acaccaggct cgacggttag cgtagcgccg accggcagcg
     6601 gcgcttcggc atcgacgcgg cccgcgcgtg gggcctcgtg cacggccagg cccacgccgt
     6661 gtcccaggcc gtgtccgaag aaatcgccgt accctgctgc gacgatggct tcccgcgcaa
     6721 gcgcatcggc ctgcacgccg ggcatgccgg cgcgcatccc gtcgagcgcg acctgctgag
     6781 cgcgtcgaac aatctcgtag acctcgtcgt atttctcatc ggcgcggccc aggctgacgg
     6841 tgcgtgtcag gtccgcgcaa tagccatcga ccttcgcgcc gaagtcgatc aagatcggct
     6901 cgccggcctg tatggcgcga tcgcccgggt gatggtgagc catggcgctg tcagggccgg
     6961 acgcaacgat cacgtcgaag gccaatgctt ccgcgccgtg cgtgcgcagg aacgcttcaa
     7021 gctcccacgc tgcctcacgc tcggtcatgc cgggcttgag gacgccgcac aggaaatcga
     7081 agcctgcgtc ggtcacggcc gccgccttgc gaatggccgc cagttccgac tcatccttga
     7141 cggcgcgaat cggctcgacg gcgttcttgg tcgcgaccca taccacaccg ccgttcatgt
     7201 tcgaccaagt cgaaaactga tccacactca cggcggcgct ctcgaatccg acgcgcttag
     7261 gatatccggc cttgcccagg atatccgcga gatgatcctc cagccgcgtc ttgatctgga
     7321 ccagctcgaa cgtcggggct tgcttcgcgg cctgttccca gtagcgcgaa tcggtggcga
     7381 ggatggaacg atcgagggta atcaacaaca cgccggcgct gcccgtgaaa ccggacaggt
     7441 accgccggtt ctccgcctgc gtcaccaaca aggcatcgat ttccaattct ctaagccggc
     7501 ctcggacctg cgccactcga tctgtcattt ccttttgctc cacagaagac gtacccttgc
     7561 gaaggtttca taaccttcgc aaggtcttaa tccgcgaccg gttccatctc tggcagctcg
     7621 ttcagcgaat actcgttctc gcaacgcagg cacctggcct ggtctttgcc cttgaccgtc
     7681 aagaggccgc cgcagttcgg gcacggctgc ggcagaggcc ggttccaact cgtgaactgg
     7741 cacgagtcgg gatcgtcgtt cttgtagttg gcacatccgt agaagatacg gccgcgcctg
     7801 gtctttttct cgaccagatc gccgccgtcg agcgggcatt tcacaccgat cttgatcagg
     7861 aacgggcgcg tgtaccggca ggtcgggaag tcgctgcagc cgatgaactt gccgaaacgg
     7921 ccccagcgaa cgatcagcgg cttgccgcac gtcgggcacg cttcgccgat ctcttcgttg
     7981 acctccacct tctcgatcgc atgcagcgcg tactcgacat ccttctcgaa cggctcgtaa
     8041 aactcgcgca ggacttcggc ccagtccttc tcgccgtcgg cgatctcgtc aagctcttct
     8101 tccatgcggg ccgtgaaccc cacgtcgata acgtcggaga aatactcgac cagcatgtcg
     8161 ttgacgatga cgccgacgtc ggtgggccac aggcgtttct cgcgcctctc gacgtacccg
     8221 cgcccctgga tcgtgctgag gataggcgcg taggtgctgg gccgcccgat cccgttctcc
     8281 tccagcgcct tcaccagcga cgcctcggtg tagcgcggcg gaggctcggt gaagtgctga
     8341 tccgggagca gcgcgagcag atcgagcaac tcgtcggcgg acagctccgg gatgcgcgct
     8401 ccctcgtcct cctccggcgc gacgtcctcg tcgcgcgctt cctcgtatac gaccaggtaa
     8461 ccggcgaact tgagcgtgga gccggtgctg cggaacaggt aggggaatcg acccgccacg
     8521 ccgttcacac tggcgtcacc caagtctcga atcacgtccg acagttctcc aacttcaccc
     8581 gccgcgacgt ccaccgacac cgtatcgtag acggctgcgt tcatttgcga agcgaggaaa
     8641 cgcttccaaa tcaggctgta cagcttgagc tgcgccgcgt cgagaaacgg cttgacggct
     8701 tccggcgtgc gccgcacgga ggtcggtcga atagcctcgt gggcctcctg cgcgttcttg
     8761 ctgcgcgtct tatattgggg aggctcgtct ggcaaatact ccgcaccgta gcgttcactg
     8821 acaaacgcgc gcgcctcctg ctgcgcctcg gctgcgacac tcgtcgaatc ggtgcgcatg
     8881 taggtgatca gacccaccga cccttcgtcg cccagcgcga tgccttcgta caactgctgc
     8941 gccagggcca tcgtgcgcct ggcggggtag ccgagtttgc ggctggcttc ctgctgcagc
     9001 gtgctggtcg tgaacggcgg gctgggccgg cggcggcgct cgcccttctt cacgccggcg
     9061 acaacgtagt cggccttctc cagctcggcg acgatcccct gggtatcggc ctcgttcttg
     9121 agatcgacgt cctgtccttt gatcttgacc agttttgcca ggaacgacgg gcgcttcttg
     9181 ggtgcgtgcg cctgcggttc gagatgcttt gccagctcgg cgtgaatcga ccagtattcg
     9241 accggaacga aggcctggat ctcgcgctcg cgctcgacga tcaggcgcaa agcgacgctc
     9301 tgcacccggc ccgcgctcag gcggctgcgc accttttccc agagcaatgg gctgagctta
     9361 tagccgacga gccgatccaa gatgcggcgg gcctgttgcg cgttcaccag ctccatattg
     9421 atgctgcgtg gatgggcgaa cgcctctccg atggcatgct cggtaatctc gtggaacacg
     9481 acgcgcttga cccgctcccg ctcgaagtcg gcggactcaa ccaggtgcca cgcgatggcc
     9541 tcgccttccc gatcgggatc ggtcgccagg taaacttcct cggcgcgctg gcccaactcc
     9601 gccagctcct tgacgacggc tttcttctcg ttcggcacgc ggtacttggg cgcgaagccg
     9661 ttgtcgacgt cgaccgacaa ctgggatctg agcaggtcgc gcacgtgccc gacggaggcc
     9721 ttcacagtgt actttcgtcc gagaaaacga ccgaccgtct tcgctttggc cggcgactcg
     9781 acgatgacca gcgcgccacg ccgctcggca ggcgcgcggt tgcgcttgcc gttgcggggc
     9841 gagcgagccg ttgcttccgg ccggggaatg ccttcgtgcg ccggcgtcca tcccatgcga
     9901 aacagcgccg tgccacactc cgggcaggcg cccttcgtcg caggcgtccc gccagccgtg
     9961 aatacgggct gcggattcac gatttcacgc ttcgttttgc attttacgca ataagcttcc
    10021 atttagctac tttcaccaca tggattcgac tcgtatgcgc ctcgagccgt cacaggatat
    10081 ccgtgcgccc ctcgtccttg agccgatcga ccattcgctt gacagcttcg gcctgatcct
    10141 tgcgacacac caggacgacg tcctcggtgt ccacgataat gacgtcttcc agcccgatgg
    10201 tcgcgacgat gcggccgcga ccgtgaacca acacgtggcg actgcctgcg ttcaggtgct
    10261 cggcgtggac gcacacgttg ccatccgcat cacctggcaa gacgtccagc agcgacgacc
    10321 acgatccgat atcgctccat ccgatatcga tcgggatgac gacgacgtcg cgcgctttct
    10381 ccatcacgcc gtaatccaca gtctggctcg cgacacgcgg ccacacgttg gcgagcgttg
    10441 aggcttcgtc cggcgtgcct agcacgcgat cgatctcgtc gagctgcgca gagaactccg
    10501 gcatcgtgcg cgccagctcg gccaggaagc gatcgacgcg ccagatgaac atgccgctgt
    10561 tccacgaatg ccgcccgtcg gcgaggaagc gctcggccgc cgccgcgcta ggcttctcgc
    10621 gaaacgacat gacctggtag gccaggaagt tctccacgct ttccacgacg tcgccgcgct
    10681 cgatgtagcc gtagccggtg gccgggtaat ccggcgtcac gccgagggtg acgagatggc
    10741 cgcgttccgc gaccttggcg gcggcgagca gcgcccggcg gaacccggcc tcgtcggcaa
    10801 tgaaatgatc ggcggtcaac acggccatca tggcgtcagg atgcttgcgc ctcaggtgga
    10861 ttgcgcccag cgcaatcgcg ggggccgtgc cgcggcccat cggctcgacg atgaaattct
    10921 cgcccggaat gccggaaccc tgcgctttca gcaagtcgac gtggttgctc cctgccacca
    10981 cgagcatgcg atccggcgtg aacagcggcg ccaggcgatc cagggcaatc tgaaacatcg
    11041 tgcggtcgcc gagcaggggc agcgcctgct tcggccggct ctcgcgggag agcggccaca
    11101 ggcgcgtgcc ggttccgcca gccatgatga gcgcgtagaa attgtcgtgc acttccaaat
    11161 cccagagttc cctgagttcc ctgagttccc tgagttccct ggatgcctta tgataaggaa
    11221 ctggggaact aaaggaactc ccgtctcctc agtccaccac gtattcggcc tgcccctcac
    11281 gcgcccgcac gtagttcatg ccgcccacct ggcgcaccag tcccttcagc tccatcagcg
    11341 ccaatacgct gctcacctgc gacacagaca ggttcatgct gcgcacgatg tcgtcgatgt
    11401 gcgtcggctc gtgcgagatc gctttgagca tcgcggtttc gaccggatcg ctcgtcagca
    11461 cggcctgagc ttccgcctgc tgagcgacca tgcgcaaatt gagcgcttcg agaacgtctt
    11521 ccgccgagcg caccagcgtc gcgccttcct ggatcaggcg attcgcacct gcgctcgtcc
    11581 ggctgaagat gctacccgga acggcgaaga cttcacgccc ctgctccagc gcgcggtccg
    11641 ccgtgatcag cgcgccgctt tgctcgcccg cttcgacgac gacggcggcc agcgacaggc
    11701 cgctgataat gcgattgcgg gccggaaagt tgccggcttc gggcggcgag ccgataggtg
    11761 tctccgtcag cagtgcgccg cgctcggcga tctgcgccgc cagacgcgca tgctcggcag
    11821 gatacaccag atcgatgccg caagccatga cgccgatcgt ccggccgccg gcccgcagcg
    11881 ccgcccagtg cgccgccgcg tcgatgccac gcgccaaccc actgacgatc gtgacgccgc
    11941 tgcgcgccag atcgctcgcc aatcgttcgg ccgcctgccg cccatacgcc gtcgcccggc
    12001 gcgtgccgac gatggacacg gcccactggc gatcggcagg cgtcagctcg cccttcacat
    12061 acagcagagg aggcgcgtca ggaatggatt gcagcagcgg cggataatca ggatcgctcc
    12121 acgtcaccag ccgcgcgccg caggccgata cacgcgcgaa ttccgcgtcg agatccatct
    12181 ttgcgcgcac agcgcgcagg ctgtcgatcg cgcgccgatc caggccggca gccttccact
    12241 cggcttcgcc cgcatgccac gccgcctgca agttgccaaa atgatccacg agcgcgcgaa
    12301 tcttggccgt tcccacgccg ggtgttcgat tgaaggcaac ccagtaacgc aaatcgtcca
    12361 tatgttcctg cctgcggtgg gtgactataa atgaatcgat aggatgtgtc aagaacccgt
    12421 tttgcggcat acacatcgct tgccggtcac tccggcagga gtccgggcaa caaggacacg
    12481 cgcatgacac ctgacgccag gtcgatgtcg cggatcactt cgacgcgcgc gggcagaagc
    12541 acctcgccgc gcgggccgtg cacgacgtag acgtcgtgcg caccggtttc gagaatctct
    12601 tcgactttgc ccagatccac gccgtcgagc gtgaccacgg ccaatccgat aatctgatgg
    12661 cggtagtact cgcggggtcg aagcgggctg gcgctgccgc gatcgatgta caggtgaagt
    12721 ccccgcagcg cttcagcctg ctcgggggtc gagtagccgg ccagcttcac gatgaggtag
    12781 tcgccctggg cgcgcgcacc ttgtatttcg caggcgcggc ggtcctcgcc cacgtacacc
    12841 tggcggcccg gtgcgatgtc ctcaggatgc gacgatagga tcttgacctt gacctcacct
    12901 gtcacgcccc aggggcgaag cacgcgcgcc acttccaaaa actcaggctc aggttgcgcc
    12961 cggccacctg agcctctcgt tgccgtcatc ttgtccgtcc gtgagcgatc gatcagagga
    13021 tatccaattg gacgcgcttg ccttctttgg cagccgccac gcgcagcagg gaacggatcg
    13081 agttcgcgac gcgcccattc ttgccgatca cgcgtcccgc gtcgtccggc gcgacgtgca
    13141 gctcgaaaat gaccgtgttg ccgctgcggc tctcttttac cgatacccgc gacgggtcgt
    13201 cgaccagcga acgcacgacg tattcgagca agtctcgcat agagccaccc gaaccgctac
    13261 gcagtggccg cggcctgcgc cttggccttc ttcttgccct tcacgccgcc gagctgccgg
    13321 gtctgggcgc tgacggcgcg cgctgcctcg ttggcttgct cggcctcggc cagcagggcg
    13381 tcgaccgctt cgccctgacg cagccgctca aagcggttca tcgtgccgcg cttggtgaag
    13441 agctgcagca cactgtccgt cggctgcgcg ccgactttca gccagtacag cgcgcggctc
    13501 tcgtcgacga ccaccgtctc cgggcttgtg cgcgggttgt acaggccgat gctttcaatg
    13561 aagcggccgt cgcgcggcgc gtcggcgtcg gccacgacga tccggtaagt gggggctttc
    13621 ttcgcgccca cgcggcgcag tctgattcga accactcttt gctcctttgc ttgtcgctta
    13681 tcgcgtatcg cttatcgcat atcgcaacac ggaacacgca tcacgtttca tgcatcacgc
    13741 ttcacgtttt acgtttcacg cttcacactt tgcgcctacc gcccacccaa cattttcaac
    13801 aggccctttc cacgtccgct gttgaactgc cgcatcatct tctgcatctc gcggaactgg
    13861 cgcacgagct ggttgacgtc ctgcaccgtc gtcccactgc cgcgcgcgat gcggcgcttg
    13921 cgcgatccgt tcaacacatc ggggttgcgc cgctcggcga cggtcatcga gtgaatgatc
    13981 gcctccgtct tcttcaactg cgtttcggcg agcgccggat cgatctgcgc cttgatctgg
    14041 cccatccccg gcagcatgtc gaggatctgc gcgaacggcc cgagcttctt catctgctgc
    14101 atctgttcga ggaaatcctc gaaatcgaac tgggctgtga tgaacttctc agccgctttc
    14161 tcggcctccg ccttgtcgaa cgcctcttca gccttctcga tcagcgtcag catatcgccc
    14221 atgccgagaa tgcgcgtcgc catacggtcg ggatggaacg gctcgagcgc atcggccttc
    14281 tcgccaaggc ccaagaactt gatcggcacg cctgtgacgg cccggatgct gatcgccgcg
    14341 ccgccgcgcg cgtcgccatc ggccttcgtc aatatcaggc cggtcagccc gacgcgcctg
    14401 tggaactcct cggcggttcg gacggcgtcc tggccggtca tcgcgtcggc gacgagcatg
    14461 acctcgcgcg gcccggccag cttcttgat
//
LOCUS       DBNHXB010000391         7177 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3129382, whole genome shotgun sequence.
ACCESSION   DBNHXB010000391 DBNHXB010000000
VERSION     DBNHXB010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3129382"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="AC162G_15815"
     CDS             complement(<1..605)
                     /locus_tag="AC162G_15815"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HIR5077322.1"
                     /translation="MKPVVVIPSFNEAENIPKLVAALFALQVPNLEMLVVDDQSPDGT
                     GEIVEQLTGCYTGRLRVLHRTGPRGLGLAYVDGFRLALADGADAIVQMDADFSHSPTD
                     VPRFLSLLKEYEVIVGSRYVVGGQLDKGWGMGRVLLSAWANFYARSILRMKTLDVTAG
                     FKCWRRSALEAIQLDRVRSNGYVFQVEMAYLCERLGLSVLEV"
     gene            complement(675..1538)
                     /locus_tag="AC162G_15820"
     CDS             complement(675..1538)
                     /locus_tag="AC162G_15820"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HIR5077323.1"
                     /translation="MADRPTILVVEDEHETAAMLTTFFEGEGYAMHWAPWGADALRIT
                     QDVQPDLIVLDIRLPDIDGYEVCRRLRAQRRTENVPIIFLTERRERVDRLSGLELGAV
                     DYITKPFDIQELRLRVRNALRRARYETLVDPITGLASEPLISEKFLSLTRQQGWAVLC
                     VGVDGLDKFADDYGFVARDDAIRALALVLSNTASEGSPDPDSHFIGQLDSGHFVIVVG
                     AGRVHDLREKLATRLKQAVNYFYPAKDRYADASKRPPLRLSLGAVTTSDGPFASPDEV
                     KTAALNALTAL"
     gene            complement(1631..2260)
                     /locus_tag="AC162G_15825"
     CDS             complement(1631..2260)
                     /locus_tag="AC162G_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V4R domain-containing protein"
                     /protein_id="HIR5077324.1"
                     /translation="MSIDKSGLYYPNRIARIYLLAMEEIMGKNGLNAVLNMAKLSHVI
                     DNYPSDNLDKQFDFSEYSALNAALEEMYGPRGGRGLALRAGRASFARGIQSFGAMAGV
                     GDLAFKVLPLGTKLKMGLPAMAGIFSQVSDQVSRVEEKDDHFLYVIERCPVCWGRNVE
                     KPVCYAAVGLLQEGLRWVSGGKEFRVDEVTCHAVGDEFCTFAVYKEPVG"
     gene            2435..4021
                     /locus_tag="AC162G_15830"
     CDS             2435..4021
                     /locus_tag="AC162G_15830"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5077325.1"
                     /translation="MLTDYRVRQREYLLKISRAMTAQLDLSSVLRLILEAAGEMLAGN
                     AGLIVLRESDAAAHSAGDATLSVRASFGIPTHLLSLFAPLWSGGIDGSAGLSRRQLAQ
                     IALAAGMPLQQVVALPMQIGDQVVGMIYIFRNYGGAFSPNDRQMLQAFADQAAVAVNN
                     AQLYQQVTEEKRRLDAILEASADGVLILDSAFRLTRFNQALARLTGATAAQAVGRHHD
                     DVIVLHNKRAGMTLSEAAAGGWPLATRGPAGLIASGPLYVEGDLQRADGSRVSVGITY
                     APLFDREGRLVNLIANVRDITRFREADELKNTFISVISHELKTPVALIKGYAGTLGRE
                     DAHWDPQTVRESATIIEEEADRLTHLIDNLLDASRLQASGLKLARSSVAVDALAARQI
                     ELFRTQTTAHTLTADFPSPFPVVHADAARIEQLLSNLIGNAIKYSPNGGAIRVSGRAK
                     PDAIEVTVSDEGIGIPVEEQSRIFERFYRADDALSRRAQGSGLGLYISKAIVEAHGGR
                     IWVNSAPGRGTAITFSLPRD"
     gene            4060..5412
                     /locus_tag="AC162G_15835"
     CDS             4060..5412
                     /locus_tag="AC162G_15835"
                     /inference="COORDINATES: protein motif:HMM:NF025711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HIR5077326.1"
                     /translation="MKFKPTPTAIRCPFCQTPISVPVHRIVDVVDQPELKSRLLSGQL
                     NAFTCLNCRNSGALTAPFMYHDADKELALIFLPMETGMSNSDQQRLIGQLTQAVMNST
                     PPEKRKAYLLQPQQFFTLQSLIDAVLSADGITPEMIQAQQARIDLLRRLLETRDEAAF
                     ETLVRENDAAIDDAFIQLVTVAMASAQADRQADEFAHLSTVRTRLLELSSAGTKIKAE
                     SDLINSVLANPTRENLLEALLKAPDSDAREALLAVGRPMLDYPFFQQLTGRIDTAKAG
                     GDAAEADRLTELRKEILALRDKLDAQAQQALAQRATILREIMVSEDIAQAMRARINLI
                     DDLFLSVLTSEMQAAQQAGDAKTFERLQQIGSAAVQAIQSRQPPELQFLNALLSAEYP
                     DQTRALLERNRQALAPELVEWLEMVAGDLREDGRQESSDHLTKVVAQARELMGATAAG
                     "
     gene            complement(5440..6093)
                     /locus_tag="AC162G_15840"
     CDS             complement(5440..6093)
                     /locus_tag="AC162G_15840"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR5077327.1"
                     /translation="MPNQAVPIVVVQDGQLAGRRWPLDKPSITLGRGEECEIVLPDRQ
                     VSRRHVRIWRAEDGFYVEDLGSKNGTYVNGIPATGRVKLQDGDEIQVALSVRLLFVGA
                     DATLPLTREMIRVSLPGLRLSKTQRRVWVGGKELDPALSLAQYRLLELLYERKGKVVT
                     RDDVIQAVWPDSDESGITEQAIDALMRRLRDRLAEADPDHEYIVTVRGHGFRLEDRV"
     gene            6210..>7177
                     /locus_tag="AC162G_15845"
     CDS             6210..>7177
                     /locus_tag="AC162G_15845"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5077328.1"
                     /translation="MLEPGSIIHDRYKIVGAIGQGGQAAVYEAEDVRLQGRRCAIKEI
                     RFDPEADEETRRQAEEQFLGEARILGRLDHPTLPKVSDYFSRGDHDYLVMDFVPGPNL
                     KEVMDEARRGGRLLPESQVLTWAAQICDALYYLHTQDPPILHRDIKPANIKLTPDGRI
                     KLVDFGLVKLMAHDEARTITVIQGRGTAAYTPLEQYGGDTGHTDVRSDIYSLGATLYH
                     LLTNQPPPDAKQRFLRPDSLTPPRALNPAISPRAEKAILSAIAMHPGDRPTSVLELRD
                     ALMQDTLEAQNVQATQARRDARADAVRRALRAHRAAALGVLIMIALA"
BASE COUNT         1357 a         2376 c         2202 g         1242 t
ORIGIN      
        1 acctccagga cgctgaggcc aagccgctcg cacaggtaag ccatctcgac ctggaacacg
       61 tagccgttgg agcgcacgcg gtccaactgg atagcctcca acgccgatcg gcgccagcac
      121 ttgaatccgg ccgtcacgtc gagcgttttc atgcgcaaaa tgcttcgcgc gtagaaattc
      181 gcccaggccg atagaagcac ccgtcccatt ccccaccctt tgtcgagctg gccccccacg
      241 acgtagcgcg agccgacgat gacttcgtat tccttgagga gcgacaagaa acgagggaca
      301 tcggtaggtg agtgagagaa gtcggcgtcc atctggacga tcgcatccgc gccatcggcc
      361 agcgccaacc gaaatccgtc gacgtaagcc aatccgaggc cgcgcggacc ggttcgatgc
      421 agcacgcgca ggcgtcccgt gtagcagcct gtcaactgct cgacaatctc gcctgtccca
      481 tcgggagact ggtcgtcgac gacgagcatc tcgagattcg gcacctgcag ggcgaacaag
      541 gcggcgacga gtttggggat gttctcggcc tcgttgaagc tggggatgac gacgacaggc
      601 ttcacgcggc ggattatacc atctcggcga tgacggcctt tgagggtttt attatccagg
      661 tagtggaggc cgcgtcacag cgcggtgaga gcgttcagcg cggctgtttt tacctcgtcg
      721 ggcgatgcga aggggccatc cgaagtggtg acggcgccca gcgacaggcg caggggcggc
      781 cgtttcgagg cgtcggcgta gcggtccttg gccggataga aatagttcac ggcctgcttc
      841 aatcgcgtcg ccagcttctc gcgcaggtcg tgcacgcgac ccgcgcccac gacgatgacg
      901 aaatggccgc tgtcgagctg gccgataaaa tgcgaatccg gatcggggct gccttccgat
      961 gcggtgttgc tcaacacgag cgccagcgcg cggatcgcat cgtctcgcgc cacgaatccg
     1021 tagtcgtccg caaacttatc gagtccgtcc acgccgacgc acagcacggc ccagccctgc
     1081 tgccgggtga gcgacaagaa cttctctgaa atcagcggct cgctcgccag accggtgatg
     1141 gggtcgacga gagtttcgta acgtgctctc ctgagcgcgt tgcgcacgcg caggcgaagc
     1201 tcttgaatat cgaatggctt ggtgatgtaa tcgacggcgc ccagctccag gcccgacagg
     1261 cggtcgacgc gctcgcggcg ctcggtcagg aagatgatcg ggacattctc ggtgcggcgc
     1321 tgggcacgca agcggcggca tacttcgtag ccgtcgatgt cgggaaggcg aatgtcgagg
     1381 acgatcagat cgggttggac atcctgagtg atgcgcagcg cgtcagcccc ccacggcgcc
     1441 cagtgcatcg cgtatccctc gccttcgaag aaggtcgtca acatggcggc agtttcgtgc
     1501 tcgtcttcga cgaccaggat agtgggtcga tctgccatgg caagttctcc gtgcggtgga
     1561 tgatcgcagc gcgggcgagg tgtgccgctc gacgcaggag ggcgaacgat gaatacagtc
     1621 cgggcaaccg tcagccgacc ggttccttgt acacggcgaa ggtgcagaac tcgtcgccga
     1681 ctgcgtggca ggtgacctcg tcaactcgaa actctttgcc tccgctgacc cagcgcaggc
     1741 cttcctgcag gaggccgacc gccgcgtagc acaccggttt ctcgacgttg cgtccccagc
     1801 acaccgggca cctctcgatg acatatagaa agtggtcgtc tttctcctcg acgcggctga
     1861 cctgatcgct gacctggctg aagatgcccg ccatcgccgg caggcccatc ttgagcttgg
     1921 tgcccagcgg cagcactttg aaagccagat cgccgacgcc cgccatcgcg ccgaagcttt
     1981 gaatcccgcg cgcgaacgac gcccggcccg cgcgcaaggc caggccgcgc ccgccgcgcg
     2041 gaccgtacat ctcttccaga gccgcattca gggcagaata ctcgctgaag tcgaactgct
     2101 tgtcgaggtt atcggatggg taattgtcga tcacgtgaga cagtttggcc atgttcaaca
     2161 cggcgttcag gccgttcttg cccatgatct cttccatcgc caggagatag atacgggcga
     2221 tcctgttggg gtaatacagt ccgctcttgt cgatcgacac gtttgaccct cccgcccaac
     2281 ggttacacgc cgcgcaggcg aagatggtga atggcgcacc acaggcctgg ccctgacctg
     2341 tgggaactaa ggctaccaca cacagtatgc gatgtcaagc caatagctct ggtctatgtt
     2401 ataatctcgc gcagcctgcc gccgaatcga ctccgtgctt accgactacc gcgttcgcca
     2461 gcgcgaatat ctcttgaaga tcagccgcgc catgacagcc cagctcgatc tgtcgtcggt
     2521 gctcaggctg attctggagg ccgccggcga aatgctggcc ggcaacgccg ggttgatcgt
     2581 cctgcgcgag agcgacgccg ctgcgcattc ggccggcgac gcgacgttgt ccgtccgggc
     2641 gagcttcggc attcccacgc atctgctcag cctgttcgct ccgctctggt cgggcggcat
     2701 cgacggcagc gccggcctgt cacggcggca gctcgcgcag atcgccctgg ccgccggcat
     2761 gccgctgcag caggttgtgg ccctgccgat gcaaatcggc gatcaagtcg tcggaatgat
     2821 ctacatcttt cgcaactacg gcggcgcgtt ttcgcccaac gaccggcaga tgctgcaggc
     2881 ctttgcggac caggcggcgg ttgccgtcaa caacgcgcag ttgtatcagc aggtgacgga
     2941 ggaaaagcgc cggctcgacg ccatcctgga agcgagtgcg gacggcgtcc tgatcttgga
     3001 ttccgctttc cggctgacgc gcttcaacca ggcgctggcg cgcctcacgg gcgccacggc
     3061 tgcccaggcg gttggccgcc accacgacga cgtgatcgtc ctgcacaaca agcgcgcggg
     3121 catgacgctg agcgaggcgg ccgccggcgg ctggccgctc gctacccgcg gccctgccgg
     3181 cctgatcgcc agcgggccgc tgtacgtcga aggtgacctg cagcgcgccg acggatcgcg
     3241 cgtgagcgtc ggcatcacct atgccccctt gttcgaccgg gaaggtcgcc tggtcaacct
     3301 gatcgccaac gtgcgcgaca tcacccgctt ccgagaagca gacgaactga agaacacctt
     3361 catctcggtt atctcgcacg agctgaagac ccccgtcgcg ctgatcaaag gctacgccgg
     3421 aacgctgggc cgcgaagatg cgcactggga cccacaaacg gttcgcgaaa gcgcgaccat
     3481 catcgaagaa gaggccgacc ggctcacaca cctgatcgac aacctgctgg atgcatcacg
     3541 cctgcaggcc agcggcctta agctcgcccg aagcagcgtc gccgtcgacg cgctggccgc
     3601 ccgccagatc gagctatttc gtactcagac gaccgcgcac accctgacgg cggatttccc
     3661 gtcacccttt ccggtcgtcc acgcagacgc ggcccgaatc gagcaattgc tgagcaatct
     3721 gatcggcaac gcgattaagt actcgccgaa cggcggcgcc atccgcgtct ccggcagagc
     3781 gaagcccgac gcgatcgagg tcaccgtcag cgacgagggc atcggcattc cggtagagga
     3841 gcagtcacgc atcttcgaac ggttctaccg cgcggacgac gcgctgagcc gtcgcgcaca
     3901 aggctcgggg ttaggtctat acattagcaa agccatcgtc gaggcgcacg gcgggcgcat
     3961 ctgggtcaat tccgcgcccg gccgcggcac ggccattacc ttctcgttgc cacgggatta
     4021 gtcgccgggg gcgatcatct cctgaaagga cttttcgaga tgaagtttaa gccaacgccg
     4081 accgcgattc ggtgcccgtt ttgccagaca ccgatcagcg tgccggtcca ccgcatcgtc
     4141 gacgtggtcg atcagccaga actgaagtcg cgccttctca gcggccagct caacgcgttc
     4201 acctgcctta actgccgcaa tagcggcgcg ctgaccgcgc cgttcatgta tcacgatgcg
     4261 gacaaagagc tggcgctgat ctttctgccg atggagaccg gcatgagcaa ctccgaccag
     4321 cagaggctca tcggccagct tactcaggcg gtcatgaata gcacgccgcc cgagaagcga
     4381 aaagcgtact tgcttcagcc gcagcagttt ttcacgctgc aatcgctgat cgacgccgtc
     4441 ctgagcgccg acggaatcac gccggagatg attcaggcgc agcaggcgcg catcgatctg
     4501 ctgcggcgtc ttctcgaaac gcgggacgaa gcggcgttcg agaccctcgt cagagaaaac
     4561 gatgccgcga tcgacgacgc cttcatccag ttggtcacgg tggccatggc cagcgcgcag
     4621 gccgaccggc aggcggacga gttcgcccac ttatcgacgg tgcgcacccg gctgctcgag
     4681 ttgtccagcg caggcacgaa gatcaaggcc gaatccgatt tgatcaactc tgtgctggcg
     4741 aacccgaccc gcgaaaactt gctcgaagcg ctgctgaaag ctcccgattc ggatgcgcgc
     4801 gaagcgctgc tcgccgtcgg ccgcccgatg ctcgactatc ccttctttca gcagctcacc
     4861 ggccggattg acaccgccaa ggcgggcggc gatgcggccg aggccgatcg gctcacggag
     4921 ctgcggaagg aaattctggc gctgcgcgac aagctcgacg cgcaggccca gcaggccctc
     4981 gcgcagcgcg cgacgatcct ccgcgagatc atggtgagcg aagacatcgc ccaggccatg
     5041 cgcgcgcgca tcaacctgat cgacgattta ttcctgtctg tcctgacgtc ggaaatgcaa
     5101 gcggcccagc aagccggcga tgcgaagact ttcgagcgac tgcagcagat cggcagcgca
     5161 gccgttcagg ccatccaatc gcggcagccc ccggagcttc aattcctcaa tgcgctgctg
     5221 agtgcggagt atcccgacca gacgcgcgcg ctgctcgagc gcaatcggca ggcgctcgcg
     5281 ccagaactgg tcgaatggct ggagatggtg gccggcgatt tgcgcgaaga cgggcgccag
     5341 gagtcatccg accatctcac caaagtcgtc gctcaggcgc gtgagctgat gggagcgacc
     5401 gccgccggat gaggcaaaga aaccgggttt cttgttcgct cagaccctat cttccaatcg
     5461 gaaaccatgc ccgcgcacgg tgacgatgta ctcgtgatcc ggatccgctt ccgccaggcg
     5521 gtcgcgcagg cggcgcatca gggcatcgat ggcctgttcc gtgatgccgg actcgtcgct
     5581 gtcgggccac accgcttgaa tcacatcgtc gcgcgtgacc accttgcctt tgcgctcgta
     5641 cagcagctcc aggagccggt attgcgctag cgacagcgcc ggatcgagct ccttgcctcc
     5701 cacccacacg cgccgctgcg tcttgctcag acggaggccg ggcaggctga cgcggatcat
     5761 ctctctcgtc aagggcagcg tcgcgtccgc gccgacgaag agcagccgca cgctcagcgc
     5821 aacctggatc tcgtcgccgt cttgcagctt cacccggccc gtcgccggaa tgccgttcac
     5881 gtaggtgccg tttttgctgc cgaggtcctc gacgtaaaaa ccgtcttcgg cgcgccagat
     5941 gcgaacgtgg cggcgactca cctgccgatc gggcaggacg atttcgcact cttcgccgcg
     6001 ccccagcgtg atcgatggct tatcgagcgg ccagcgccgg ccggccagtt gaccatcctg
     6061 caccaccaca ataggcaccg cttggtttgg catgctccct ccagtgaggc ctgaggcgct
     6121 gatgttttag cggccacggc gcggccacgg gaccccttga caatcctacc tcactcggct
     6181 aagcttgcaa gcgaggagaa agagagggat tgctcgaacc cggatcgatt attcacgacc
     6241 gctacaaaat tgtcggcgct atcgggcaag gtggtcaagc ggccgtctac gaagccgaag
     6301 atgtccggct gcaaggccgc cgctgtgcga ttaaagagat ccgcttcgat cccgaggccg
     6361 acgaagagac gcgccgccag gctgaggaac agttccttgg cgaagcgcgc attcttgggc
     6421 gcctcgatca tcccactcta cccaaagtct ccgactactt ctcgcgaggc gaccacgact
     6481 acctcgtgat ggacttcgtg cctggcccca atctcaagga agtgatggac gaagcccgcc
     6541 gaggtggccg cctgctgccc gaatcgcagg tgctgacctg ggccgctcaa atctgcgatg
     6601 ccctgtacta tctgcatacc caggacccgc ccatcttgca ccgcgacatc aagccggcca
     6661 acatcaagct gacgccggac ggccgcatca agctcgtcga tttcggcctg gttaagctga
     6721 tggcgcacga cgaagcccgc acgatcaccg tgattcaggg tcgcggtaca gccgcgtaca
     6781 cgccgctcga gcaatacgga ggcgataccg gccatacgga cgtgcgcagc gacatttact
     6841 cgctcggcgc gacgctgtac cacctgctca cgaaccagcc gccgcccgat gcgaagcaac
     6901 ggtttctgcg gcctgattcg ctgacgccgc cccgcgcact gaacccggcg atttcgcctc
     6961 gcgccgaaaa agccatcctg tccgccatcg ccatgcaccc gggcgaccgt cccacatcgg
     7021 tcctggagct tcgagatgcg ctgatgcagg atacgctgga agcgcagaac gttcaggcca
     7081 cgcaggcgcg ccgggacgcg cgcgcggacg ccgtgcgccg cgcgttacgc gcccaccgcg
     7141 cggctgccct cggcgtcctc atcatgatcg cgctggc
//
LOCUS       DBNHXB010000392         1985 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2460687, whole genome shotgun sequence.
ACCESSION   DBNHXB010000392 DBNHXB010000000
VERSION     DBNHXB010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2460687"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="AC162G_15850"
     CDS             <1..538
                     /locus_tag="AC162G_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077329.1"
                     /translation="HLRERVSARWSRWLANLLIAVVVGYNLVGTALAVQQPHGITTQF
                     DRVTWLERGREQALIDFLLAHGETRGYSNYWVAYPIAFLSNERILSAPRLPYHLDFSY
                     TRRDNRVARYAEAAAESPRAFYITTSHPQLDALIRDRLLALGVTFREESIGSYHIFYA
                     LSRKVEPDMLGLGETTPR"
     gene            535..>1985
                     /locus_tag="AC162G_15855"
     CDS             535..>1985
                     /locus_tag="AC162G_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077330.1"
                     /translation="MNRRSTSPAQFLLPRLEDVVFVTVFVAVIALGPRLLNGDGDLGR
                     HITIGGYILESRSIPTRDVFSHTMTGEPLTPHEWLSQIVFALAYRFMGLDGVVLVCAL
                     LIASTFTLLYKNSVRRSGARLVALGWVMLAVAASRLHWLARPHLFTMLFVVLWTDGLE
                     RLRQGGSSRWQHLPALMLLWVNIHGAFIAGFVIWAAYLVDYAWQRWIARRDAEGSARY
                     GRQLVLIGAASFLASFLNPAGAELWTTSLGYVSNRYLVGHTIEYLSPDFHEVNTWPFL
                     LMIVLSMMLLGTALVRVPTASLLLLAGWTALGLYSARNVPLYAVIAAPILAVVVGASM
                     RTGTRLARWQGVETRVAAIQTLLRGHVWPVMTTLLLTFLLANGAKIDVTRRGNRFDPA
                     VFPVQAVDWLEANPLPGRIFNYFTWGGYLLYRMWPEQTVFIDGQTDFYGEALTRKYEQ
                     VITLADGWQDVLRDYRVDWVLMPSDSRLARSLTV"
BASE COUNT          316 a          607 c          629 g          433 t
ORIGIN      
        1 ccacttgcgg gaaagggtga gcgccaggtg gtcaaggtgg ctcgccaacc tgttgattgc
       61 agtcgtcgtc gggtacaatc tggtcggcac ggcgctggcc gtgcagcagc cgcacggtat
      121 cacgacgcag ttcgaccgcg tgacgtggct cgagcgcggc cgcgagcaag ccttgattga
      181 tttcttgctc gcgcacggcg agacgcgcgg ctactctaat tactgggtcg cataccccat
      241 cgccttcctg tcgaacgagc gtatcctcag cgcgccgcgc ttgccatacc atctcgattt
      301 cagctacacg cggcgtgaca atcgcgtcgc gcgctacgcc gaggcggcgg cggaatcgcc
      361 ccgcgcattt tacatcacga cgagccatcc gcagcttgac gcgttgatcc gggatcggct
      421 tttggcgctg ggcgtcacgt ttcgggaaga atcgatcggc agctatcaca tcttctacgc
      481 tctgtcgcgc aaggtcgagc cggacatgct cggcctcggt gagacgactc cccgatgaat
      541 cgacgcagta cttctcctgc acaattcttg ctgccccgtc tggaagatgt cgtgttcgtc
      601 accgtcttcg ttgcggtgat cgccctcggg ccgcgtttgc tcaacggcga cggcgatctg
      661 ggccggcaca tcaccatcgg tggatacatc ctggaaagcc gcagcattcc cacgcgcgac
      721 gttttttcgc acacgatgac cggcgagccg ctcacgccgc acgagtggct gtcccaaatc
      781 gtcttcgcgc ttgcctaccg gttcatgggg ctggatggcg tggtcttggt gtgcgcccta
      841 ttgatcgcct ctacattcac actgctgtat aagaacagcg tgcggcgcag cggggcgcgc
      901 ctcgtcgcgc tggggtgggt tatgcttgca gtggcggcgt ccaggctgca ctggctcgca
      961 cgcccacatt tgttcacgat gttgttcgtc gtgttgtgga cggacggcct ggaacgcctg
     1021 cggcaaggtg gctcaagtcg ctggcagcat ttgccggcgc tgatgctgct gtgggtcaat
     1081 attcacggcg cgttcatcgc cgggttcgtg atttgggctg cgtacctggt tgattacgcg
     1141 tggcagcgct ggatcgcgcg acgggatgcg gaaggctcgg ctcgatacgg ccgccagctc
     1201 gtgctgatcg gcgcggcatc gttcctggcg tcgtttctca atccagccgg cgcagagctg
     1261 tggacgacga gcctgggcta cgtgagcaat cgatatctcg tcgggcacac catcgagtac
     1321 ttatcaccgg attttcacga ggtcaacaca tggcctttct tgctcatgat cgtgctgtcg
     1381 atgatgctgc tcgggaccgc gctggtacgg gtgccgactg cttcactcct gctgttggcc
     1441 ggatggactg cgctcgggct ctatagcgct cggaacgtgc cactgtacgc cgtaattgct
     1501 gcgccgatcc tggccgtcgt ggttggggcg agcatgcgca ccggcactcg cctggcgcgc
     1561 tggcagggcg tggagacgcg cgtagccgcg atccagacgt tgctgcgcgg tcacgtgtgg
     1621 ccggtcatga cgacccttct ccttacgttc ctgctggcaa atggcgcgaa gatcgacgta
     1681 acccgtcgtg gcaaccgttt cgacccggcc gtgtttcccg tgcaagccgt cgactggctg
     1741 gaggcgaacc ctctaccagg acggatattc aactacttca cgtggggcgg ctatctcctg
     1801 tatcggatgt ggccggaaca gacggtcttc atcgacgggc agaccgactt ctacggcgaa
     1861 gccctcaccc gcaagtacga acaggtcatc acgcttgcgg atggctggca ggatgtcctg
     1921 cgcgattatc gagtggactg ggtcctgatg ccgtctgatt cgagattggc gcgctccctg
     1981 acagt
//
LOCUS       DBNHXB010000393         4738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2125844, whole genome shotgun sequence.
ACCESSION   DBNHXB010000393 DBNHXB010000000
VERSION     DBNHXB010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2125844"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            420..1901
                     /locus_tag="AC162G_15860"
     CDS             420..1901
                     /locus_tag="AC162G_15860"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5077331.1"
                     /translation="MHLRHRRPPLRSTISAYALAILALIATTGVLLALRGGLASPVIV
                     LLYLLLVVLTTVLRGLGPGLLVAVCAFLAFNFFFIPPYYTFAVHESSDVIALVVFFIV
                     AVVISQLLGRAQAGVAAAMAREQEATRLYELSTALAGLQDEQAIATTLATQVLDAFRA
                     THVEVGVAAHPNAKDYEHHLPLDAPPPARDPDLVVPMLTARGRQGEVRVWRSARPITA
                     PEERLLRTFASQGALAIERARLSQAETRAKVLEESDRMKSALLSSVSHELRTPLATIK
                     AAVTSLRSQTVSWESEARTELLVAVEEESDYLNLLVGNLLDMSRIEAGALKPQRQWDI
                     LSEIVFGVTRRLRRITERRRLDIDMPDDLPLVPVDHMQMEQVFINLFDNSLKYAPPDT
                     TIRVHARIQDESFLLVQVSNEGPPVPEQHLEHIFEKFHRVTDADRVTGTGLGLSICKG
                     IVEAHGGRIWAENLTDGFAFNFTLPLTWDGAPPPRLPLEPDKL"
     gene            1898..2593
                     /locus_tag="AC162G_15865"
     CDS             1898..2593
                     /locus_tag="AC162G_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5077332.1"
                     /translation="MNSAPHILVVDDEPQILRALRTILTEKKFQVSTASRGQEALALA
                     AAHPPDVVILDLGLPDLDGIEVCTRLREWTQVPIIVLSVRESDRDKVTALDRGADDYL
                     TKPFSIEELLARIRVALRHSAQAQGSKEPVITTTRFVIDLARHVVTCEGEEVKLTPTE
                     FKLLAYLGVNAGRVLTHRSILTHVWGEAEADHTEYLRVYVRQLRKKLEADPEQPQYLL
                     TEPGVGYRFNAEE"
     gene            2733..3437
                     /locus_tag="AC162G_15870"
     CDS             2733..3437
                     /locus_tag="AC162G_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077333.1"
                     /translation="MTHQPNVSPRTKLLASGTSHHEEAEAPGIPWVTLEDDLIPPARR
                     LVVLVFDAEDAEMELARRIWLLASPRGLDVLYLSLNEDPSEEPLARRRLATLAALTRD
                     ARTRVETMLLFDHDWRVAVRRIAQPQDVIICSSEQTVPMRGHKRQSLDQVLACELQVP
                     VVVLLNHTRSTPHPPATAATRHARGALPYLIVAGFFVLQLQIAQLPHDPTYYVLMCLS
                     VLIEAGLIMMCVRSED"
     gene            3518..>4738
                     /locus_tag="AC162G_15875"
     CDS             3518..>4738
                     /locus_tag="AC162G_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5077334.1"
                     /translation="MATTTHRRFSLAQLMFGPPLRTADIPHQTLSKKIGLAVFASDNL
                     SSVAYATEEILIVLSLAGAAYFYLSIPISIIIATMLVILTISYRQTIYAYPGGGGAYI
                     VARDNLGEAPAQTAGAALLTDYILTVAVSISSGVAQIASAFPALYPWRVEIALGMIAI
                     MTIVNLRGVKESGRVFSIPTYFFIVLMFFTLGRGFIQLATGTLGTVTGVERVAGEEHA
                     LQALTILLLLRAFASGSTAVTGVEAISNGITAFKEPRSKNAAQTLLVMAAILGTTFVG
                     ITLLANHIHVVAGEDVTETVISQVARTIHGEGLLYLLTVLATTVILIMAANTSYADFP
                     RLAALHAGDGFLPKQLTFRGRRLVFSWGILTLAAVAALLIILFNAETTRLIPLYAIGV
                     FLSFTLSQSGMVVRW"
BASE COUNT          866 a         1481 c         1441 g          950 t
ORIGIN      
        1 gttagtggaa ctcctcgggc gtgaagcgtt tgttgtccag gcgtaagcga ccatgcgatg
       61 gccgaggtcg cgtgacatcc cgttcaacga ttcgggcgct gatggcgcga aagtcaggct
      121 agtcgtaagg cgtatcgtgt tcgccagcgg ttgctcttca ggaggtggcc tgtgatccgc
      181 atcggaactt ctggcttctc atacaaagat tgggccgggc cggtctatgt ggtcaggccg
      241 ccctgagcgc gcgggatctg caccaactgc tcgcgcaaga agtgacctga ccggtgggac
      301 gactcaacct caaacgattg tcggctactc cttcctccac acgcccacga tagatcgtct
      361 gcactgtgga aatggtagac ttgtgataca atgtgttcga cttatgcaaa gagtggttcg
      421 tgcacttgcg ccaccgtcgc cctccgcttc gatcgaccat ttccgcgtac gctttagcca
      481 ttctcgccct cattgccacg acgggcgttt tgctggctct gagaggcgga ctggccagtc
      541 ccgtgattgt gcttctgtac cttctgcttg tcgtgctcac cacggtgctg aggggattgg
      601 ggcctggttt gctcgtcgca gtgtgcgcct ttctggcttt taacttcttc ttcattcccc
      661 cctactatac ctttgccgtt cacgaatcgt ccgacgtgat cgccttggtc gtgttcttca
      721 tcgtggccgt cgtcatcagc caactgcttg ggcgagcaca ggccggcgtg gccgcggcga
      781 tggcccgcga gcaggaagcc acgcgcctgt acgaactgag cacggcgctg gccgggctgc
      841 aagacgaaca ggccatagcg actaccctcg cgacacaggt gctcgacgcc tttcgcgcca
      901 cgcatgtcga ggtcggtgtg gcagcgcatc ccaacgcaaa ggattacgag catcacctgc
      961 cgctcgacgc accgccgccg gcgcgggatc ccgatctcgt cgtgccgatg ctcacggcgc
     1021 gtggacggca gggcgaggtg cgcgtgtggc gcagtgcacg ccccatcacg gcgccggaag
     1081 aacgtctgct gcgcaccttt gccagccagg gtgcgctcgc catagaacgc gcccgcttga
     1141 gccaggctga aacgcgagcg aaagtgctgg aagaaagcga tcgcatgaag tcggcgctcc
     1201 tgtcgtctgt gtcacacgag ctgcgcacgc ctttggccac catcaaggcc gccgtgacca
     1261 gcctgcgcag ccagacggtg agctgggagt ccgaggcgcg caccgagctg ctcgtggctg
     1321 tggaggagga atccgactac ctgaacctgc tggtcggaaa cttgctagac atgtcgcgaa
     1381 tcgaagccgg cgcactcaag ccccagcgcc agtgggacat tctgtccgag attgtctttg
     1441 gcgtcacgcg gcggctgcgg cggatcaccg aacggcgccg gcttgacatt gatatgcctg
     1501 acgatcttcc gctcgtgccg gtcgatcaca tgcaaatgga gcaggtcttc atcaatctgt
     1561 tcgacaattc gctcaagtac gcgccgccgg acaccacgat ccgcgtgcac gctcgcatcc
     1621 aggacgagtc gtttctgctc gtccaggtca gcaacgaagg gccgccggtc ccggaacaac
     1681 atctggagca catcttcgag aagttccatc gtgtcacaga cgccgaccgg gtcaccggaa
     1741 ccgggctggg gctttccatc tgcaagggta tcgtcgaggc tcacggcggt cgcatctggg
     1801 ccgagaacct gaccgacggt ttcgccttca acttcacgct gccgctgacc tgggatggcg
     1861 ctcccccacc tcgcttgcct ctcgaaccag acaaactatg aacagcgctc cccatatcct
     1921 cgttgtagac gacgaacctc aaatcctgcg cgcgctgcgc actatcttga cggagaagaa
     1981 gttccaggtc agcacggcca gtcgcggtca ggaggcgctg gcactggcgg cggctcatcc
     2041 acccgacgtc gtgatcctcg atctgggcct accggatttg gacggtatcg aagtctgcac
     2101 ccgtttgcgg gagtggacgc aagtccccat catcgtgctt tcggttcgcg agagcgaccg
     2161 cgacaaggtc acggcgttgg atcgaggcgc agacgactat ctcaccaagc cgttcagcat
     2221 cgaggaattg ttggcacgca tacgcgtggc gctgcggcat tcggcgcaag cgcagggcag
     2281 caaggaaccc gtaatcacca cgacccggtt cgtcatcgat ctggcccgac acgtcgtcac
     2341 ctgcgaaggt gaggaagtga aactcacgcc caccgagttc aagttgctgg cttacctggg
     2401 cgtgaatgcc gggcgtgtat tgacccaccg cagcatcctg acgcatgtgt ggggcgaggc
     2461 ggaggctgac cacacggaat acctgcgcgt gtatgtgcgc caactgcgca agaaactgga
     2521 ggccgacccg gaacagcccc agtacctgct gaccgaaccc ggcgtcggct accgtttcaa
     2581 tgctgaggaa tagaccaggc gattgcacta aaaacgcccc gtgaccgggg cgtttttatt
     2641 tgtcctttat ggaattgccc gaaatctttg tcggcattta atgtgcgttg attacactga
     2701 cctctgatag cggaaagagc aagcaagcgg acatgacaca ccagccgaac gtatcgccgc
     2761 gtacgaagct tttggcgtcg ggcacaagcc accacgagga ggccgaagcg ccgggcattc
     2821 cgtgggtgac cctggaagat gacctcatcc caccagcacg ccgcttggtc gtcctggtgt
     2881 ttgatgcgga ggacgccgag atggaactcg ctcggcgcat ctggctgctt gcttcgccgc
     2941 gcggtctcga cgtgctgtat ctcagcttga acgaggaccc aagcgaagaa ccgttggcgc
     3001 gccggcggct ggcgacgctg gccgcgctca cccgtgacgc tcgaacgcgc gttgagacca
     3061 tgttgttatt cgaccacgat tggcgcgtgg ccgtgcgccg tattgcacag ccgcaagatg
     3121 tcattatctg ctcgtcggag cagaccgtac ctatgcgcgg ccacaagcgg cagtccctag
     3181 atcaggtgct ggcctgcgaa ctgcaagtgc ccgtggtcgt gctgttgaat cacacgcgga
     3241 gtacgcctca ccctccggca acagccgcca cgcggcacgc gcgcggcgct ttgccgtact
     3301 tgatcgtcgc cgggttcttc gtgctgcaat tgcagatcgc gcagttacct cacgatccga
     3361 cgtactacgt actgatgtgc ctctcggtgc tgatagaagc ggggctgatc atgatgtgcg
     3421 tccgttccga agactgagtg agtcaagctg ctccgccagc tggcggagcg tgctcaggac
     3481 aacccggcgc gcacactcga gaggaaggag tctgtctttg gcaaccacaa cgcatcgacg
     3541 cttttcactg gcacaattga tgttcggccc gccgctgcgc acggccgata tcccgcacca
     3601 gacgttaagc aagaagatcg gcctggccgt gttcgcttcc gacaacctgt cgtcggtggc
     3661 ctacgcaacg gaagaaattc tgatcgtgtt gtcgctggcc ggggcagcgt acttctactt
     3721 gtcgatcccg atctcgatca tcatcgccac aatgttggtc atcctgacga tctcgtaccg
     3781 ccagaccatc tacgcttacc cggggggcgg cggtgcgtac atcgtggcgc gcgacaacct
     3841 gggtgaagcg ccggcgcaga cggccggcgc agcgctgctg accgattaca tactgaccgt
     3901 cgccgtgagc atctcctccg gcgtggcgca aatcgcatcg gcttttccgg cactctaccc
     3961 gtggcgtgtc gagatcgcgc tgggcatgat cgcgatcatg acgatcgtga atctgcgcgg
     4021 agtgaaagag tccggacggg ttttctccat tccgacctat ttcttcatcg tcctgatgtt
     4081 cttcacgctg ggaagaggat ttatccaact ggccaccggc acgctaggta cggtgaccgg
     4141 cgtcgagcgc gtggcgggag aagagcacgc gctgcaggcg ctgacgatct tgctgctgct
     4201 gcgcgccttc gccagcggct cgacggcggt gaccggcgtc gaggcgatct ccaacggcat
     4261 caccgcgttc aaagagccgc gcagcaagaa cgccgcccag acgctgctcg tcatggcggc
     4321 gatcctggga acgacctttg tcggcatcac gctgctggcg aaccacattc acgttgtggc
     4381 cggcgaagac gtaacagaga cggtcatttc gcaggtggcg cgcaccattc acggcgaggg
     4441 gctgctctac ctgctgacgg tgctcgcgac gacggtcatc ctgatcatgg cggccaacac
     4501 ctcgtacgcg gacttcccac gcctggccgc gctgcacgcc ggcgacggct tcctgcccaa
     4561 acagttgact tttcgcggcc ggcggctggt gttctcttgg ggcattctga cgctcgcggc
     4621 ggtggccgcg ctgctgatca tcctcttcaa tgccgagacg acgcgattga tcccgctgta
     4681 cgccatcggc gtgttcctct cgttcacgct gtcccagtcc ggcatggttg tgcgctgg
//
LOCUS       DBNHXB010000394         4755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4641886, whole genome shotgun sequence.
ACCESSION   DBNHXB010000394 DBNHXB010000000
VERSION     DBNHXB010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4641886"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="AC162G_15880"
     CDS             complement(<1..1197)
                     /locus_tag="AC162G_15880"
                     /inference="COORDINATES: protein motif:HMM:NF051246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR5077335.1"
                     /translation="MSIDGITRSVVSLYNPTTITRTFEYTQVGIGPHVLQVRLASGRA
                     TVDAFITPPAVRPVADFTAAPLVGTAPLTVTLTNQSIPTRTIISYLWDFGDGVTSTLQ
                     HPVHAYTDLGAYTVQLTANTERHSDTETKVGYVTVVPTSAVVITYTYDGLYRLTNATY
                     SGAATATFDYAYDAAGNRTAMTKTITSTLVTTHTYDAANRLATQNGQSLFTWDANGNL
                     LNDGSATYAYDFANRLISTTLGGATTQFGYNGEGIRLRLIEAGTLTTYTQDYAAPLPV
                     VLQAKTGTTSTQYVYSLGTRPIGEYEAAAWEYLLADPLGSVRQIADPSGNVTLLKSYE
                     PYGNALNSQGSAISIFAYAGEQIDATGLVFLRARYMQPRLGAFLSRDVWEGDMLRPGS
                     MNGWNYV"
     gene            complement(1490..2056)
                     /locus_tag="AC162G_15885"
     CDS             complement(1490..2056)
                     /locus_tag="AC162G_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077336.1"
                     /translation="MTKTIDVEFEMSAARTMLAELEEYLGSDVLYWQTASNALGDRMP
                     KLTIGGLLEALLRAEAAGAPDAPALRSSLQRIKSGHLYRYLDRAERETRSRLDAWAWY
                     LDDYARSAADAAGYYSREVRARLKAELLLSEVEPHQRGRAERARADALDERVRAAFAP
                     GEFVLDERLKAYLPADRFWWLYGRLHNR"
     gene            complement(2125..3540)
                     /locus_tag="AC162G_15890"
     CDS             complement(2125..3540)
                     /locus_tag="AC162G_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5077337.1"
                     /translation="MNEPAGATGYQPPPNGWRTFLIVWVTQSVSVFGSALTFFAVNIW
                     LAQTLYPRPEQKSELAAALSAIALAFALPTVFGAPIAGAWADRHDRKRTMMAMDFANA
                     LLSLLLAALVINNALQLPLLVVLVAIQALFGVFHQSAFDTSYAMIVPEDKLPRANGMM
                     QTIWALSGIVSPAIAAAMISVPALLRQSGVTGGVAQLRDGTPLVMGIDALTFFAASAV
                     LAFLYVPSPKRGDLQAGDGTARKSLWADIREGALYIWHRRPLLWLLGTFTVANFVAGP
                     LGVFQPLILKFNLAADWSSRGFTFETALALLASVGSVGGVAGGVFISAWGGLKRKRVY
                     GVVVSLIAAGLALIVYGLSGALYLTAAMAFLDTAMIPIMNAHSQAIWQTQTPHELQGR
                     VFAVRRLIAQFTWPLSTALAGWAGGAFNPGAVIAVLGGVLTLFCVGQLLNPYLMRVED
                     KAWLDEMAARRVAAGGQQANG"
     gene            complement(3624..4478)
                     /locus_tag="AC162G_15895"
     CDS             complement(3624..4478)
                     /locus_tag="AC162G_15895"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077338.1"
                     /translation="MTQSTQSLLTGPAAWISSLVAMSRKELTIMVRYPVEFVASFGQI
                     FLIVIVLTLAGLMFSPNGVQSGGSSQTSGIVVYGFILFMFLSDTLWTIGYNVRREQKQ
                     GTLEQLYLSPASKSASLVSRVTITLVWTGLLSVASAALMSVMLGRLPFHNLPLGAFIL
                     VLALSGTFGVGFAFAALTLRIKEAGQTMANLIQFAFMMLCAPFFPFSALPEWIQNISR
                     LIPMSYAVDVFRSTLMGYPEGFPELASIEAELVIVTAFGLLTPVLGLWLYRREEDRAR
                     RTGSLSEY"
     gene            complement(4567..>4755)
                     /locus_tag="AC162G_15900"
     CDS             complement(4567..>4755)
                     /locus_tag="AC162G_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077339.1"
                     /translation="FPPTWMLNGVRSALLGVGYFFETWYLDMAVLWAFMFFAPLFGFW
                     AFHRVENGIRRNQGMGEF"
BASE COUNT          883 a         1550 c         1529 g          793 t
ORIGIN      
        1 cacatagttc cacccattca tcgaccccgg ccgcagcata tccccttccc acacatcccg
       61 tgaaaggaac gcccccaatc tcggctgcat ataacgcgcc cgcaggaaaa ctaaccccgt
      121 cgcatcgatc tgctcgcccg cgtaagcgaa gatagatatc gcgctgccct gactattcag
      181 cgcgttgcca tacggctcgt acgacttcag cagcgtcacg ttgccgcttg ggtcggcgat
      241 ctggcgcacg ctgcccaatg gatccgccag caagtattcc cacgccgccg cctcatactc
      301 tccgatcggc cgcgtgccca ggctgtacac gtactgcgtg ctggtcgtgc ccgtcttggc
      361 ttgcagcacg accggcagcg gcgcggcata gtcttgcgtg taggttgtta aggtgccagc
      421 ctcaataagc cgcaggcgga tgccttctcc attgtatcca aattgcgtag tcgcgccacc
      481 cagggtcgtg ctgatcaggc ggttggcgaa gtcgtaggca tacgtcgcgc tgccgtcgtt
      541 gagcaggttg ccgttggcat cccaggtgaa gaggctctgg ccattctgcg tggccagccg
      601 gttcgcggca tcataggtgt gcgtcgtcac cagcgtcgag gtgatggtct tggtcatcgc
      661 cgtgcggttg ccagcggcgt cgtaggcgta gtcgaaggtg gcggtggccg ccccggagta
      721 ggtcgcgttg gtcaggcgat acagcccgtc gtaggtgtaa gtgatgacca ccgcgctcgt
      781 cgggacgacg gtcacgtaac cgaccttcgt ctcggtgtcg ctgtgccgct cagtgttggc
      841 cgtcaactgg acggtatacg cgccaaggtc cgtgtaggca tggaccgggt gctgcagcgt
      901 gctggttacg ccatcgccga agtcccacag gtaggaaatg atagtgcgcg tgggtatcga
      961 ctggttggtg agggtgacgg tcagcggcgc cgtgcccacg agcggcgcgg cggtgaagtc
     1021 cgctaccggc cgaacggcgg gcggcgtgat gaaggcgtcc accgtggccc ggccgctggc
     1081 caggcgcacc tgcagcacgt gcggcccaat cccgacctgg gtatactcga acgtgcgggt
     1141 gatggtggtg gggttgtaca ggctgacgac gctgcgcgtg atgccgtcga tcgacacttc
     1201 gacctggccg gcccaggaaa gtcctcaccc gactcacctc aagttgaacg cagaaacgaa
     1261 atgccctctc ccgaaatcga gacagggcaa ggtgtcgtgt ctgcttgcag cgtctaatgg
     1321 gtaaggccaa gggcgcagtc aggcgccccg cacccatctg agacacctac ggttcacgcc
     1381 gccctctgac cgccggatgg atcacgcgaa gcggagcggg agcggggaaa accttgaagt
     1441 tcttggtgtc tttgtgtccc ttcggctccg ctcagggcag gcttggtggt taacgattat
     1501 gcagccgccc gtacagccac cagaatcgat cggccggcag gtatgccttc aatcgctcgt
     1561 ccaggacgaa ttcccccggc gcgaacgccg cgcgcacgcg ctcgtcgagc gcatccgcac
     1621 gcgcccgctc ggcgcggccg cgctgatgcg gctccacttc gctcaagagc agctcggcct
     1681 tgagccgcgc ccggacttcg cgggagtagt agcctgccgc gtcggctgcg ctgcgggcgt
     1741 agtcgtccaa ataccaggcc cacgcgtcga gccggctgcg cgtttcgcgc tcggcgcggt
     1801 cgagataccg gtacaagtgt ccggacttga tgcgctgcag ggacgagcgc agcgcgggcg
     1861 cgtccggtgc gccggccgcc tcggcgcgca gcagcgcttc gagcaggccg ccgatcgtca
     1921 gcttcggcat gcggtcgccc agcgcgttcg aggccgtctg ccagtagagc acgtcggacc
     1981 cgaggtactc ctccagctcg gcaagcatcg tgcgcgctgc gctcatttcg aattcgacgt
     2041 cgattgtctt ggtcatgtcg gcgcgagctc caagtttcat ggcatggata ggtgacggct
     2101 cattcaggcc ccgcggtgcg agccttatcc gttcgcttgc tgcccacccg ctgcgaccct
     2161 gcgcgccgcc atttcgtcca gccacgcctt gtcctcaacg cgcatcaggt acggattgag
     2221 caactgtccc acgcagaaca gcgtcaggac cccgccgagc acggcgatca ccgcgccggg
     2281 attgaacgcg ccgcccgccc acccggccag cgccgtgctg agcggccagg tgaactgcgc
     2341 gatcagccgc cgtaccgcga acacgcgccc ctgcagctcg tgcggcgtct gcgtctgcca
     2401 gatcgcctga gagtgtgcgt tcatgatcgg gatcatggcc gtgtcgagga acgccatcgc
     2461 cgctgtgaga tacagcgcgc ccgataggcc atagacgatc agcgcgaggc cggcggcgat
     2521 cagcgagacg acgacgccgt acacgcgctt gcgcttcaac ccgccccacg cgctgatgaa
     2581 tacgccgccc gccacaccgc cgacgctgcc gaccgacgcc agcagcgcca gcgccgtctc
     2641 gaaggtgaac ccgcgcgacg accagtcggc ggcaagattg aacttgagga tgagcggttg
     2701 gaacacgccc agcggcccgg cgacgaagtt ggcgacggtg aaggtgccca atagccacag
     2761 cagcgggcgg cgatgccaga tgtacagcgc accctctcgg atgtcggccc acaggctctt
     2821 cctggctgtg ccatcgcctg cctgcaagtc gcctcgtttc ggcgatggca cgtacaagaa
     2881 ggccaagact gccgaggccg cgaagaaggt gagcgcgtcg atgcccatga ccagcggcgt
     2941 cccgtcgcgg agctgggcaa cgccgcctgt cacgccgctc tgccgcagga gggccggcac
     3001 ggagatcatc gccgcggcga tagcgggcga cacaatgccc gacagcgccc agatcgtctg
     3061 catcatgccg ttggcgcgcg gcagcttgtc ctctggaacg atcatcgcgt acgacgtgtc
     3121 aaacgccgac tgatgaaaca cgccaaagag ggcctggatg gcgacgagga cgacgagcag
     3181 cggcagttgc agcgcgttat tgatcaccag cgcggccagc agcaggctca gcagggcatt
     3241 ggcgaagtcc atcgccatca tcgttcgctt gcggtcgtgg cgatcggccc acgcacccgc
     3301 gatgggcgcg ccgaacaccg tcggcagcgc gaaggcgagc gcgatcgccg acagggcggc
     3361 ggcgagctcc gacttctgct ccgggcgcgg atacagcgtc tgcgccagcc agatattgac
     3421 ggcgaagaac gtcagcgcgc tgccgaacac cgacaccgac tgcgtgaccc atacgatcaa
     3481 gaacgtgcgc cagccgttcg gcggtggttg ataacctgtc gcgcctgcag gttcgttcat
     3541 gcctcggctc ctggccggca cccccgccga tggccgcggg cgaggatgtc agcgggaggg
     3601 gtctgatctc cccttgtcga agatcaatat tctgaaaggc tgcccgtacg ccgcgcgcgg
     3661 tcttcctcgc gccgatacag ccacagcccc aaaaccggcg tcagcaggcc gaacgccgtg
     3721 acgatgacca gctcggcttc gatggaggcc agttccggga agccctccgg gtagcccatc
     3781 aaggtcgagc ggaagacatc gacggcatac gacatcggga tcaggcgcga gatattctgt
     3841 atccactccg gcagcgccga gaaggggaaa aacggcgcgc acagcatcat gaacgcgaac
     3901 tggatcaggt tcgccatggt ctgccctgct tccttgatac gcagcgtcag cgcggcgaag
     3961 gcgaatccca cgccgaatgt gccggacagc gccaggacca ggatgaaggc gccgagcggc
     4021 aggttgtgaa acggcaggcg gcccagcatg acgctcatca gcgcggccga cgcgacgctc
     4081 agcaggccgg tccacaccag ggtgatggtg acgcgcgaga ccaggctggc cgatttggac
     4141 gccgggctga ggtacaactg ctcgagcgtg ccttgtttct gctcacgccg gacgttgtat
     4201 cctatcgtcc acagagtgtc acttaagaac atgaacagga tgaagccgta cacgacgatg
     4261 ccggacgtct ggctgctgcc gccggactgc acgccgttcg gcgagaacat caggccggcc
     4321 agcgtgagca cgatgacgat cagaaatatc tgcccgaagc tggcgacgaa ctcgaccggg
     4381 tagcgcacca tgatcgtcag ctctttgcgc gacatggcga cgagcgaact gatccacgca
     4441 gcagggcctg tcagtaaaga ctgagttgat tgtgtcattc gcgccgtcct tccatgcgtt
     4501 tcgtattgcg tgttgcgtgg cacacgttga acacggagcg ataagccata cgctatacgc
     4561 gataggctag aactccccca tgccctgatt gcggcggatg ccgttctcca cgcgatggaa
     4621 agcccagaat ccgaacagcg gcgcgaagaa catgaacgcc cacagcacgg ccatgtcgag
     4681 ataccacgtc tcgaagaagt agccgacgcc gagcagcgcc gagcgcacgc cgttcagcat
     4741 ccacgtcggc gggaa
//
LOCUS       DBNHXB010000395         4500 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3131146, whole genome shotgun sequence.
ACCESSION   DBNHXB010000395 DBNHXB010000000
VERSION     DBNHXB010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3131146"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            437..1669
                     /gene="recA"
                     /locus_tag="AC162G_15905"
     CDS             437..1669
                     /gene="recA"
                     /locus_tag="AC162G_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR5077340.1"
                     /translation="MAPRKRKQAAEEEIAEESSSVVVKPADPGRVRALDQALGNILKR
                     FGDGAIMRLGEATHMQVETIPTGSLALDLALGAGGIPRGRVTEIYGPEASGKTTLCQH
                     IVAEAQKRGGIAAYIDMEHALDPTYAARCGVDIDNLYIAQPDTGEQALEIAESLVRSG
                     AVDVIALDSVAALVPRAEIEGEMGDSHMGLQARLMSQALRKLSGAIKQSNTAMIFTNQ
                     LRMKIGVMFGNPETTTGGNALKFYATIRLDMRRTQSIKSGAEIVGNRVKVRVTKNKIA
                     PPFREAEFDIMYNEGISKTGDILDIGAAMEIVTKRGAFFSFNDTKLGQGRESAKEFLK
                     SHPEVASEIEAVIRDKVKSEAAAVMAAPSSNGRNGQRGAAQHEEPDEDADEEMAALEE
                     DAHDSGAEKEQMAEEVAV"
     gene            1835..2812
                     /locus_tag="AC162G_15910"
     CDS             1835..2812
                     /locus_tag="AC162G_15910"
                     /inference="COORDINATES: protein motif:HMM:NF012784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR5077341.1"
                     /translation="MSHAFREVAVSENGVLSLKDIKPKMKFEGKVTRVELAGALVDIG
                     AVTDAFLHISQIKADPPVTRVADVLHEGDAITVWVKQVKPQQNLIALTMVAPPALDWG
                     DLQKRMKLTGKVVRVEEFGAFVNIGAPKDGLVPVSQMAKTRVAKPSDVVKEGDEVTVW
                     VTSASRKENRIGLTMVEPPAVDWSEIKRGQTVTGKVTRLERYGAFVDIGAEREAMIHV
                     SEISPGYVGHPGDLLEVGEEIEAQVLEVDPRKKQIKLSIKAVERSAVAEAQPEAALLS
                     PMEIAFRQAQGQAQPAAKRPAPVEPASADTRKEQEDIFRRTIEQHVSKP"
     gene            complement(2929..3183)
                     /locus_tag="AC162G_15915"
     CDS             complement(2929..3183)
                     /locus_tag="AC162G_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077342.1"
                     /translation="MPRIRCHYEDCIFLDAGFCSAEKIELDPESGCLTYQQAEEAELV
                     IADWEEEEADAEVPDDEEDEVEWEEEEDEDDDEEDDEDEW"
     gene            complement(3267..3740)
                     /locus_tag="AC162G_15920"
     CDS             complement(3267..3740)
                     /locus_tag="AC162G_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HIR5077343.1"
                     /translation="MSSRWTAHRTALLQMLLSAVGVVVAVVLTSFHYGPATTAALCTG
                     AGGCETVNTSPYSTIAGIPVALLGMGAYVAIGALAFLSTREGALAEQAHLAVFGLSLA
                     GVLYSAYLTYLEIFVIRAICPWCVVSALIMTAIWISSVVDLVRRRRAAAEVNASP"
     gene            complement(3883..>4500)
                     /locus_tag="AC162G_15925"
     CDS             complement(3883..>4500)
                     /locus_tag="AC162G_15925"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR5077344.1"
                     /translation="ILGAVIVVLGLIVLSGGLAPPAAEVTTESLELCNGKPCPSKGSP
                     DAPVVVIEFSDYGCHNCRDFNLTTAPILERDYVDAGKVRYVSHVFSLWPESIPAAAAS
                     LCAHEQGKYWEFHEQAFRDFQQGRFPASEDFLNWAQLIGLDQASFKQCVDSGRTVYDA
                     QVSALEGTRAGISGTPSFYINGTLVSGNVPLDEFRRTIDAALAGQ"
BASE COUNT          942 a         1422 c         1416 g          720 t
ORIGIN      
        1 tagagatgcg tcgtcgcgct acgcgctcat acccagaaac ctgccatacc gtgtcattcc
       61 gaacgcagtg aggaatctct gccacaaccg gctatgacgg cacgccatgg tagagatgct
      121 tcgcgaaaag atgctcagca tgacacggcc gggcgtttct gtccgcacgc ataccgatgg
      181 cggtatggac ttctcggcat gacacgcctg aattatatac ccggcggaat ccggcgcaaa
      241 ccgcactcgt ccgccaggtg tgtcactccg tgtatccgtg cccaaatccg tgtcatgctg
      301 tgtttgtggc gatctgaaca atcaccagaa aatggcggtt gacagaacag aacatttgtg
      361 ctagtatata tggcacatcc gagccatccg tccgatcggc acatcacgca cactgtaacc
      421 cttgagggag gcacccatgg caccacgaaa gcgcaagcag gcggcagaag aagaaatcgc
      481 agaagaatcc agctctgtgg tcgttaagcc cgccgatccg ggccgcgttc gcgccctgga
      541 tcaggcgctg ggcaatattc ttaaacgttt tggagatgga gcgatcatgc ggctgggcga
      601 ggctactcac atgcaagtcg aaaccatccc gaccggctct ctggcgctcg acctggcact
      661 gggcgcgggc ggcatcccgc gcggccgggt gaccgagatc tacgggcctg aggcgtccgg
      721 caagacgacg ctgtgccagc acatcgtcgc cgaggcgcag aagcgcggcg ggatcgccgc
      781 gtacatcgac atggagcacg cgctcgatcc gacctacgca gcgcgctgcg gcgtggacat
      841 cgacaacctg tacattgccc agcccgacac cggcgagcag gcgctcgaaa tcgccgagag
      901 cctggtgcgc tcgggcgcgg tggacgtcat cgcgctcgac tcggtcgcgg cgctggtgcc
      961 gcgcgccgag atcgagggcg agatgggcga cagtcacatg ggtctccagg cgcggctgat
     1021 gtcgcaggcg ctgcggaagc tgtcaggcgc gatcaagcag tcgaacacgg ccatgatctt
     1081 caccaaccag ttgcgcatga agatcggcgt catgttcggc aatccggaga ccacgacggg
     1141 cggcaacgcg ctgaagttct acgcaacgat ccgcctggac atgcgccgca cgcagagcat
     1201 caaatccggc gccgagatcg tgggcaaccg cgtcaaagtg cgcgtgacga agaacaagat
     1261 cgccccgccc ttccgcgaag ccgagttcga catcatgtac aacgagggca tctcgaagac
     1321 cggcgacatc ctggacatcg gcgcggcgat ggagatcgtc accaagcgcg gcgcgttctt
     1381 ctccttcaac gacaccaagc tgggccaggg acgcgagagc gcgaaggagt tcctcaagag
     1441 ccatcccgaa gtcgcgagcg agatcgaggc cgtgatccgc gacaaggtga agagcgaagc
     1501 agcggccgtg atggccgcgc cgtcgtcgaa cggcaggaac ggccagcgcg gcgcggctca
     1561 gcacgaggaa cctgacgaag acgcggacga agaaatggcc gcgctggaag aggacgcgca
     1621 cgattccggc gccgagaagg aacagatggc agaagaggtg gccgtctagc agcttgcctg
     1681 ccctgagcga aggcgaaggg tcggagagag agcaggccgg gctcccacgc ccggcaattg
     1741 gccttcacac gatgcgcgct gtgtttgaca cccccctcac ggcgcggtat aatccccgct
     1801 gggtgataat gccccgttca aacggggcat tatcatgtca catgcttttc gcgaggttgc
     1861 cgtgtccgag aatggggtcc tctccttgaa ggatatcaag ccgaaaatga agttcgaggg
     1921 caaggtcacc cgggtcgagc tggcgggcgc gctcgtcgac atcggcgccg tgacggatgc
     1981 cttcctccac atctcgcaaa tcaaagccga cccgcccgtg acgcgcgtgg ctgacgtgct
     2041 gcacgaaggc gacgcgatca ccgtgtgggt caaacaggtc aagccgcagc agaatctcat
     2101 cgcgctgacg atggtcgcgc cgccggcgct cgattggggc gacctgcaga agcgcatgaa
     2161 gctgacgggc aaagtcgttc gcgtggagga gttcggcgcg ttcgtcaaca ttggcgcgcc
     2221 caaggacggc ctggttccgg tgagccagat ggcgaagacg cgcgttgcca agccgtcgga
     2281 cgtcgtcaag gagggcgacg aggtcacggt gtgggtcacg tcggcgagcc gcaaggagaa
     2341 ccgcatcggc ctgacgatgg tcgagccgcc ggccgtggac tggagcgaga tcaagcgcgg
     2401 ccagacggtg acaggcaaag tcactcgtct cgaaaggtat ggcgcgttcg tcgacatcgg
     2461 cgccgagcgc gaagcgatga ttcacgtcag cgagatcagc ccgggatacg tcggccatcc
     2521 gggcgacctg ctcgaggtcg gcgaagaaat cgaggcccag gtcctggaag tcgacccgcg
     2581 caagaaacag atcaagctat cgatcaaggc ggtcgagcgg agcgccgtgg ccgaagcgca
     2641 gcccgaagcg gcgctgctct cgccgatgga aatcgcgttc cgccaggcgc agggacaggc
     2701 tcagccagcc gcgaagcggc cagcgccggt ggagccagcc agcgcagaca cgcgcaagga
     2761 acaagaggac atcttccggc ggacgatcga gcagcacgtc tccaaaccct gatcgaagca
     2821 caggccggtg aacataaaga gcgggctttc ttgcagaagc ccgctctttg tgattcgaga
     2881 cagtccgcac acgaggcgat acgccggcgc agtcgccgca gccgtcagct accactcatc
     2941 ctcgtcgtct tcttcgtcgt cgtcctcatc ctcttcttcc tcccattcca cttcatcctc
     3001 ttcgtcgtcc gggacttcgg cgtcggcttc ctcctcctcc cagtcggcga tgaccagctc
     3061 ggcctcttcg gcctgctgat acgtcaggca gcccgattcg ggatcgagct cgatcttttc
     3121 ggccgagcaa aacccggcgt cgaggaagat acagtcttca tagtggcatc ggatgcgcgg
     3181 cataagctca ttcccccgga agcgtgtccg aaacgactgg ccgtatgata acgcaaattc
     3241 gcgctatcgc aaagagggcg acggcctcac ggcgaggcgt tgacttcggc agcggcgcgt
     3301 ctgcgccgga cgagatccac caccgaagaa atccatatcg ccgtcatgat cagcgccgat
     3361 acgacgcacc acgggcagat ggcgcggatc acgaagatct ccaggtaggt cagatacgcg
     3421 gagtacaaca cgccggcaag tgacagaccg aatacggcga ggtgcgcctg ctcggcgagc
     3481 gcgccctcgc gcgtcgacag gaaagccagc gcgccgatcg cgacgtaggc gcccatgccg
     3541 agcagcgcaa caggaatgcc cgcgatcgtc gagtacgggc tggtgttcac cgtctcgcag
     3601 ccgcccgcgc ccgtgcacag cgccgccgtc gtcgccgggc cgtagtggaa cgacgtgagg
     3661 accacggcga ccacgacgcc gacagccgac aggagcatct gaagcaatgc agtgcggtga
     3721 gccgtccacc gagatgacac accgatttcc ccagagacgt cttttggctc cgaaattggc
     3781 gggtatcccg cttccggcct gggcggctgc aagttcattt tgcaccttga ctgtgccgga
     3841 atacttgcca gtccgtgtaa tccgtgggaa atccgtgtgt catcattggc ctgccagcgc
     3901 cgcgtcgatc gtgcggcgga actcgtccag cgggacgttg ccggacacca gcgtgccgtt
     3961 gatgtagaac gacggcgtgc cgctgatgcc ggcgcgcgtg ccctccagcg ccgacacttg
     4021 agcgtcgtac acggtgcgac ctgaatcgac acattgcttg aacgacgcct ggtcaagccc
     4081 gatgagctgc gcccagttca gaaaatcctc actcgccgga aagcggccct gctgaaaatc
     4141 tcgaaacgcc tgctcgtgaa actcccagta tttgccttgc tcgtgtgcgc acagcgaggc
     4201 cgcggcagcc ggaatcgact ccggccagag cgagaacacg tgcgacacgt agcgcacctt
     4261 gcccgcatcg acgtaatccc gctcgagaat cggcgccgtc gtcaggttga agtcccggca
     4321 gttgtgacac ccataatccg aaaactcgat gacgacgacc ggtgcgtcgg gcgatccctt
     4381 agacgggcac ggctttccat tgcacagctc cagcgactcc gtcgtcactt ccgccgccgg
     4441 cggggccaat ccgccgctca acacgatcag gcccaatacc acgatcacgg cgcccagaat
//
LOCUS       DBNHXB010000396        14205 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_112702, whole genome shotgun sequence.
ACCESSION   DBNHXB010000396 DBNHXB010000000
VERSION     DBNHXB010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 14205)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14205)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14205
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_112702"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1061)
                     /locus_tag="AC162G_15930"
     CDS             complement(147..1061)
                     /locus_tag="AC162G_15930"
                     /inference="COORDINATES: protein motif:HMM:NF017020.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR5077345.1"
                     /translation="MTISLTSFRTMTQAEALDRIRGDLLRARPDVVGIIAFGSFARGE
                     DWRDVDVLVVLPTLRNDPAGWAETAHALRQAVDLPRVEVIPYSQPGFINGLRNHSPFL
                     LDVALDGIVLHDRANLAEAIASVKRDLQERGIRRLRPGSWRFPVQYRRSTLLSERRNE
                     EYVRLWLDDADRDAQAAAALRSVGLHDRSVYHCQQAVERAVKACLACFGAFERTHFIA
                     REMRLAINDQSLGEWADRLRRVAAVAERLEPHVARARYVIEDAEGEKWWVPADQYSDS
                     DSATALNETREAIRIVHDFVDWWFAPER"
     gene            complement(1293..2075)
                     /locus_tag="AC162G_15935"
     CDS             complement(1293..2075)
                     /locus_tag="AC162G_15935"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077346.1"
                     /translation="MSEAALRAEHIRHVFHDANGHTEALDDISLEITPGEFACLVGPS
                     GSGKSTLLRILAGLIQPVAGRIWLDGQLVQAPSRSVCIVFQKANLMPWRTVLDNIALP
                     LEIEGLPQPERISRAASWVERVGLGGFERAYPHSLSGGMEQRVAIARALISNPSVLLL
                     DEPFGSLDALTRERMALELTRIWSKQRTTVIMVTHSIQEAVFLSDRVLVLTQRPGRIA
                     ADIPIPFRRPRDLNRLYQEAFGEIVGRLRATIEAHEGVAAIR"
     gene            complement(2072..3061)
                     /locus_tag="AC162G_15940"
     CDS             complement(2072..3061)
                     /locus_tag="AC162G_15940"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5077347.1"
                     /translation="MKRIVMWLVVASVWMTGCAGAAATPASLTRIRVPMGFIANVQYA
                     PFYVAVDKGYFAEEGIEIEFDYRFETDGMKLVGAGELPFAVVSGEQVPLAREQGLPVV
                     YVLQWFQKFPIAIVAPASKGIRTPADLVGRQVGLPGFFGASYVGWRGLLSQAGIRESD
                     VKTQDIGFTQVAALQQGAVDAAVGYVNNEPIQLEAAGIDVDVIPVSDYVSMVANGIVT
                     NEKTIGENPGLVRRFVRALLRGLEDTIADPEAAFTISTKFVEGLGANPDSDVIQREVL
                     AKSIDQWRGEPLGRSDAAAWDTTQDVLISMGLLKQKIDSAQLFTNRFVDEVSR"
     gene            complement(3148..3906)
                     /locus_tag="AC162G_15945"
     CDS             complement(3148..3906)
                     /locus_tag="AC162G_15945"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077348.1"
                     /translation="MQRRRIRRSLLAPLTLLGFVVAWEALVRIRDYPAFILPSPGDVL
                     AELAQSVLTPSFWAHIGVTLTEVTGGLLIGASLAIVLGYLVAKSATLDAMLSPLFVAS
                     QAVPIVAVAPLLTIWFGYGLTPKIVTSALIVFFPVLINVSAGIRSVEPNLRDLMRSLQ
                     ANRLQMLSKLEAPAALPMALSGLRVGATLAVIGAIVGEFVNSDRGLGFLIKQGIGEYN
                     TAATFATLIALAAIALSLYGAVALLERRWLAWRR"
     regulatory      complement(4146..4286)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(4409..5077)
                     /locus_tag="AC162G_15950"
     CDS             complement(4409..5077)
                     /locus_tag="AC162G_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIR5077349.1"
                     /translation="MDHDMALYLKPGRFIDSDNPRVIAFAREHAGNGDSRGRAVRLYY
                     AVRDSIAYNPYRIEPVAESFRGGRTLETGYGWCVSKAALLAAVARAIGIPARIGFADV
                     RNHLTSPRLREWLGGTDVFAYHGFCELSIDGSWMKATPAFDKALCVRANVAPLEFDGM
                     HDSLFQQFDASGRRHMEYLTMRGVFADVPFEQIIADWRVLYPNVFARSRDATPDFADE
                     LERQ"
     gene            complement(5367..6185)
                     /locus_tag="AC162G_15955"
     CDS             complement(5367..6185)
                     /locus_tag="AC162G_15955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5077350.1"
                     /translation="MMPQTVQAPRTQVHFRPHLAAVTSGETAREQWVAFIGKLSLEKG
                     VHCLIAAAPAIVAKVPQARFILIGDGVARAHLVAMRDALSEGDLDAAARAMQHAAETS
                     PEPYAEWVADYWAGLDLHQYHKMAAAADLRKRVVFTGYQPAPDVARLISHVDLVVIPS
                     LIKEAFPLVSVEALATGLMFVAPHVGGLAPVLDRVSAELDPIGRLASLRYGPQHLIPD
                     MIDQVSTLLRYTSQEARRRELMKQCRNFALRHYDWTNIVSRIESVYVSALYGYH"
     gene            complement(6427..7608)
                     /locus_tag="AC162G_15960"
     CDS             complement(6427..7608)
                     /locus_tag="AC162G_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR5077351.1"
                     /translation="MKLPGRERAEPEAPVTHEKSRLCPECCTPVSTRATTCAICGYDF
                     VAAEQAERAEQAELREEAAQRPVRAIAIGVAAAVALLLIAALYFRNRAEAIAALTPTA
                     TPTPTATPTPSRTPTPTPTPLFTPTPIPPREYVVQPGDNIFYIADIFQVDYRDLLAFN
                     NLTENSVLQVSQKILIPPPTATPTPSLVPQDATAAPDSGSTAIVHVVQAGETLIGIAQ
                     EYGVTVSAILQVNQIEDANQIQVGDQLIIPQAGATTVTPGSVAETPQPNYGPVTLLQP
                     LNGSRITGNERNVLLQWLSAGILDEDETYRVMLEQVDGDIRYGPVYIKATALHVPLEL
                     FPAPDDPHRTFRWTVTIMHQVGVGSDGAPLYAVISPSSSRTFHWMPALPTPTLTPNPS
                     P"
     gene            complement(7605..8180)
                     /locus_tag="AC162G_15965"
     CDS             complement(7605..8180)
                     /locus_tag="AC162G_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HIR5077352.1"
                     /translation="MNRDAAWILVTEWTQSDSLRKHMLAVEAGMRAYARRFGEDEEKW
                     ATVGVLHDFDYERYPDVAAEGHPVVGSRVLREMGWPDEIVRCILSHATEVTRVERRTL
                     MEKALYAVDELTGLITAVTLVRPGKNIADVQVSSVKKKWKDRAFAAGVHREEIERAAA
                     DLGVPLDEHIGVVLAAMQEAAPALGLDGSAR"
     gene            complement(8314..11013)
                     /locus_tag="AC162G_15970"
     CDS             complement(8314..11013)
                     /locus_tag="AC162G_15970"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR5077353.1"
                     /translation="MAEHSMPKTYDFKTTEQRLYAWWEKNGWFKPEIARRRHADRFVI
                     SMPPPNVTGELHIGHAMTASVEDCLIRYHRMRGDRTLWLPGTDHAGIATQLQVEKHIL
                     RTEGVTREQLGREEFIRRTWAWKEKYGSYIAEQHKRLGVSADWDRERFTLDKGLSRAV
                     REAFVRLYEKGLIYRGTYLVNWSPGLKTAVSDLEVEYSEEQGTLYYFKYRLAGHRDAY
                     IPVATTRPETILGDTAVAVHPDDERYRHLIGETVLVPILNRTIPVIHDTMVDPGFATG
                     ALKITPGHDLTDYEIGQRHGLPIVSVMNPDATMNENAGPYQGLDRFECRKRMWADMEA
                     AGLAIKTEPYPMTVPRSQRGGEIIEPLISTQWYVKIQPLAEPALEAVRSGKIRIIPER
                     FAKVYFNWLDASRLRDWCISRQLWWGHRIPVWTCKDCRHEWAAREDPYSCAKCGRHNI
                     EQDPDVLDTWFSSGLWPFSTLGWPDETPDYKDFYPTSVMETGYDILFFWVARMIMLGL
                     EMTGKPPFHTVYLHGLIRDEHGRKMSKTLGNTIDPTTIMDQYGTDALRLTLLTGSTPG
                     NDMNFSSQRAEANRNFANKIWNAARFVIGHLDVGSWRLEVGKGLSNLQSPISNLQLPD
                     RWILSRLNRLIADVTNLFGDYQLGEAGRLIHEFLWSEFADWYIEASKLPLRSGDAEAR
                     KRTLSILVHVLDQALRLLHPFLPFVTEEVWQHLKKAAGDKNWPAALIVAPWPEPGATD
                     EAAERDMGVLMEAIRAIRNARAEHDVKPENRLAAMLISGDKANLFEAHRDVMALLARL
                     DADGLHIAPGLKAIPDRALSLVMASATVYLPLAQMVDLDTERSRLNREFADAEAQITR
                     STALLTGDFVTRAPAAVVQKERDKLAALQNKRAKLEAQIKSLM"
     gene            complement(11463..12095)
                     /locus_tag="AC162G_15975"
     CDS             complement(11463..12095)
                     /locus_tag="AC162G_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077354.1"
                     /translation="MIEVRTVYQVKFGKIDQAVELFRRLTSYLPEEVAATAHYHLLSD
                     ISGPMYTLAEEFVLPSLTEWEASRERLFNHPEFANWFKGFQLFIDNGRQEFYTVEGEC
                     EDWSRTGVIVVRQNYRARQWQIRQAVDLLRRYGALMVDSGVGRKPRITTDVSGPMFQA
                     VIEVEVDNLADWEAHRRSMFLRPEFQVWFVQLMNAVEAGAHEFFRVEVAG"
     gene            complement(12117..12749)
                     /locus_tag="AC162G_15980"
     CDS             complement(12117..12749)
                     /locus_tag="AC162G_15980"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HIR5077355.1"
                     /translation="MNADTPVEAPPVLAEAVPPPPSRTRLRLVRILTVLILIGAVAGT
                     LYIVSDPGRLRRVEELITSPSGLVVLFVFGAITSATLVLPVPGLALTAIAGSVANPFV
                     VGIVAGAGQTLGEMTGYMAGYSGQTLIDDSPRYARLVSWMRRFGAPIIFVLALLPIAL
                     FDVAGIIAGALRMPVWLYLLSASAGKIIKNVAVAYAAAYGMEWLKQLFNL"
     gene            complement(12774..13283)
                     /locus_tag="AC162G_15985"
     CDS             complement(12774..13283)
                     /locus_tag="AC162G_15985"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5077356.1"
                     /translation="MAKRYSAPPPMVIDPQKKYTATIVTPIGDVVIELFADKVPRTVN
                     NFVFLARDGYYAGVTFHRVIQGFMAQGGDPTGTGRGGPGYTFGDEFHPQLRHDAPGIL
                     SMANAGPNTNGSQFFITHGPTPHLDNRHSVFGRVTQGMDVVLKIPERDPARAIEPGLA
                     MTRIDIVES"
     gene            complement(13367..>14205)
                     /locus_tag="AC162G_15990"
     CDS             complement(13367..>14205)
                     /locus_tag="AC162G_15990"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR5077357.1"
                     /translation="GQVESKGWTIKNTPKVVGALTPQGARRTAALLETMGAPVHVVSS
                     PRAAELSKLLENTFRSVNIALVNELALLCERMGIDVWEVIAAAATKPFGFMPFRPGPG
                     VGGHCIPVDPLYLSWKAREFDFHTNFIDLAAQVNDDMPYHVVDLIVRGLSKQGRALDG
                     ASVLVLGVAFKRDIDDARNSPAERVMELLMARGAAVCYHDPHVPEFHAGGNVFHRQRE
                     RLASIPLTDDAVSSADCIVVVTGHSAVDYRRVAGRARLLVDTCNVVPRDLEATIVRLG
                     AP"
BASE COUNT         2587 a         4493 c         4451 g         2674 t
ORIGIN      
        1 agcccatctg caaacttcgc ccctccgata ccaccagcta acgcgacaac tagcattgat
       61 tatcgtttgg tctcactttc tgccttgtgg cgtttgaaca catataatcc ctcgtctgga
      121 catcagttcc ttccagactc taggtgctac ctctccggag caaaccacca atctacgaaa
      181 tcatgcacga tacgaatcgc ctcgcgcgtc tcattcaacg cggttgccga gtctgaatcg
      241 ctgtactgat cggccggaac ccaccacttc tcgccctcgg catcctcaat cacgtagcgc
      301 gcgcgtgcga catgaggttc caggcgctcg gcaacggccg ccacgcgcct caggcgatcc
      361 gcccattcgc cgagcgattg gtcgttgatg gccagccgca tttctcgggc gataaaatgg
      421 gtgcgctcga aggcgccgaa acaggccagg caggctttca cagcgcgctc aacagcctgc
      481 tggcagtggt atacacttcg gtcgtggagg ccgaccgagc gcaaagcagc cgcagcctgc
      541 gcgtcgcgat cggcatcgtc cagccagagt cgaacatact cttcgttgcg cctttccgat
      601 aggagcgtgc ttctccggta ttgcaccgga aatcgccaac tgccgggtcg gaggcggcgg
      661 atgcctcgtt cctgaaggtc gcgtttgacg cttgcgattg cctccgccag attggcccgg
      721 tcgtgcagga cgattccatc cagcgccacg tctagcagga aaggcgaatg atttcgcaag
      781 ccgttgataa accccggctg cgaataaggg attacttcga cgcgcggcag atcgacggcc
      841 tgccgaagtg cgtgagcggt ctccgcccac ccggccggat cgttcctgag cgttggcagc
      901 accacgagta catcgacgtc gcgccagtct tcgccgcgcg caaacgagcc aaatgcgata
      961 atgccgacaa cgtccgggcg tgcccgcagt aggtcacccc ggatgcggtc gagtgcttct
     1021 gcctgagtca tcgttcgaaa gctggtcagc gagatcgtca ttgtcctcgt ctccggtatg
     1081 tgcgtatcgg ccaatcgcct gagcaagatt gcgcatagat agaagcgacc ttgcccacaa
     1141 ggattgtagc acgatctgac cggttgatca ggaacggtgg actcaaacgt gaggtgtgcg
     1201 tgggcgatgg agttgtgcag cggcagtagc gccgatcacg gactttgcgt ctttgtagtg
     1261 aagcaacgcc ttgtgatcac gcgaccagcc ggtcaccgaa tcgctgcgac tccctcgtgt
     1321 gcttcgatcg tcgcgcgcag ccgcccaacg atttcaccga acgcttcctg gtagagtcgg
     1381 ttgagatccc ggggccgacg gaatgggatc gggatgtcgg cggcgatgcg gccgggtcgc
     1441 tgcgtcagca ccaggacgcg atcgctcaag aagaccgctt cctggatgct gtgtgtgacc
     1501 atgattaccg tcgtgcgctg tttactccag atgcgcgtca gctccagcgc catgcgctcg
     1561 cgcgtgagcg cgtcgagcga tccgaacggc tcgtcgagga ggaggacgga ggggtttgag
     1621 atgagcgcgc gggcgatggc gacgcgctgc tccatgccgc cggagaggct gtgagggtaa
     1681 gcgcgctcga agccgcccag gccgacgcgc tcgacccagc ttgccgcccg gcttatgcgc
     1741 tccggctgcg gcaagccctc gatttccagc ggcagcgcga tgttgtcgag caccgtgcgc
     1801 cacggcatca ggttggcttt ctggaagacg atgcaaaccg agcggctggg cgcctgcacc
     1861 aactgcccgt cgagccagat gcggcccgct accggctgga tcaacccggc caggatgcgc
     1921 agcagcgtcg atttgccgct gcccgacggc ccgaccaggc acgcgaactc gcccggcgtg
     1981 atctcgagcg agatgtcgtc cagcgcttcc gtatggccgt tagcgtcgtg gaagacgtga
     2041 cggatgtgct cggcccgcag cgccgcttcg ctcaccggct gacctcgtcc acgaagcggt
     2101 tggtgaacag ttgcgcgctg tcgatcttct gtttgagcag gcccatgctg atcagcacgt
     2161 cctgcgtcgt gtcccacgcc gccgcgtccg atcgcccgag tggctcgccg cgccactggt
     2221 cgatcgattt ggcgagcact tcgcgctgaa tcacgtccga atcgggattc gcgccgagtc
     2281 cctcgacgaa cttggtcgaa atggtgaacg cggcctcagg gtcggcgatc gtgtcttcca
     2341 ggccgcgcag cagcgcccgc acgaagcggc gcaccagacc cggattttcg ccgatcgtct
     2401 tctcgttcgt cacgatgccg ttggcgacca tcgacacgta gtcgctcacg gggatcacat
     2461 cgacgtcgat gccggccgct tcgagctgaa tcggctcgtt gttcacgtag ccaactgccg
     2521 cgtcaactgc gccctgctgc agcgccgcga cctgtgtgaa gccgatgtcc tgcgtcttga
     2581 cgtccgattc gcggatgcca gcctggctca gaagcccgcg ccagccgacg taactcgcgc
     2641 cgaagaaccc tggcaggccg acctgcctgc cgaccaggtc ggcaggagtc cggatgcctt
     2701 tgctcgcagg cgcgacaatc gcgatcggaa acttctggaa ccactgcaag acgtacacga
     2761 cgggcaggcc ctgctcgcgc gccagtggca cctgctcgcc gctgacgact gcgaacggca
     2821 gctcgccggc tccgaccagt ttcatgccgt ccgtctcgaa gcggtagtcg aattcaatct
     2881 cgatgccctc ttcggcgaaa tagcccttgt cgacggcgac gtagaacgga gcgtattgca
     2941 cgttggcgat gaagcccatc ggcacacgga tcctggtcag cgatgcgggc gtcgccgccg
     3001 cgcccgcaca tcctgtcatc cagacagatg cgactaccag ccacatcacg atgcgtttca
     3061 tactctcttc tccccgataa gacctggcag gtcttcccga tccagcctcc ggataacagg
     3121 cggacaagac ctgccaggtc taaggagtta tcgtctccac gccagccagc gccgctccag
     3181 cagcgccacc gccccgtaca gcgacagggc aatcgccgcc agcgcgatca atgtggcgaa
     3241 cgtggcggcc gtgttgtact cgccgatgcc ctgcttgatc aggaagccca gtcctctgtc
     3301 tgagttgacg aactcgccga cgatcgcgcc gatcacagcc agcgtcgcgc cgactctgag
     3361 gccgctcaac gccatcggca aggccgccgg cgcttccagc ttggacagca tctggaggcg
     3421 attggcttgc aagctgcgca tcaggtcgcg caggttcggc tcgacggagc ggatgccggc
     3481 cgagacattg atcagcaccg gaaagaagac gatcagcgcg ctcgtgacaa ttttgggcgt
     3541 caggccgtag ccgaaccaga tggtcaagag aggcgcgacg gcgacaatgg ggacagcctg
     3601 cgaggcgacg aacaagggcg acagcatcgc gtcgagcgtc gccgacttgg cgacgaggta
     3661 gccgagaacg atcgcaaggc tcgcgccgat gagcaggccg cccgttacct cggtcagcgt
     3721 gacgccgatg tgcgcccaga acgacggtgt gagaaccgac tgcgccagct ccgccagcac
     3781 gtcgcctggc gatggaagaa tgaaggccgg atagtcgcga atgcgaacca gcgcctccca
     3841 ggcaacgacg aatcccagca gcgtcagcgg cgcgaggagg ctgcgcctga tgcgccttcg
     3901 ctgcacatgt ggaccgccgt gtgacgactc ctgatccaac ctggtcgtca cagcctgtcc
     3961 tgagcgtagc ggccttgaga gtgccatgag tcacctcgca acaaaaaacc cgaagcgcag
     4021 agacggcctt cgggacgagc tgacgcagcg caaccacgat gcccccgaac ctgatcgaaa
     4081 accccgaagc cataaaaact ggctccgggg caggcaatcg agcacatgca cggaacgcgc
     4141 aagcgcgtga ccttctttca tccggactat accgtcggct ccggaattgc accggatcat
     4201 gccccagtat gaggctcgcg ggctgtaccg ccgatcggga attgcagctc gtatgacgag
     4261 ctactcaccc tgccccgaag gtggattatt cacttatttc aaattatacc gttcggaaat
     4321 gcaattgtca actgcaccct aacccaactt tgcgccgtgg aagaccaatg cgccggtggc
     4381 gaagaagacg caatcctcgc cagcccggct attgtctctc aagctcgtcg gcaaaatcgg
     4441 gagtggcgtc acgcgaacgg gcgaaaacgt tgggatacag caccctccaa tcggctatga
     4501 tctgctcgaa cgggacatct gcaaatacgc cgcgcatggt gagatactcc atgtggcggc
     4561 gcccgcttgc atcaaactgc tgaaacagcg aatcgtgcat tccgtcgaac tccaggggag
     4621 cgacgttggc gcgcacgcac agcgccttgt caaaggcggg agtggctttc atccagcttc
     4681 catcgatcga cagctcgcaa aaaccgtggt aggcgaacac gtcggtgccc cccaaccatt
     4741 cgcgcagccg tggcgacgtc aaatggttgc gcacatcggc aaacccgata cgcgccggaa
     4801 ttccgatggc gcgcgccact gcagcgagca gcgcggcctt ggaaacacac cagccatacc
     4861 cggtctctaa tgtccgcccg ccgcgaaacg actctgctac aggctcaatc cgatacgggt
     4921 tatatgcgat gctgtcccgc acggcataat acaggcgcac cgcgcgcccg cggctgtcgc
     4981 cgttcccggc gtgctctcgg gcgaacgcta tcaccctggg attatcgctg tcgatgaatc
     5041 gcccgggctt caggtagagc gccatatcgt gatccattct tgtctgactc catctcacct
     5101 gccggcaact gactgtggtt gtgccgtatg acccgcgctc gttccggcaa gagtctatga
     5161 catattgggc gccaagtcaa gtaccttgac ccgggcacat cgcgccgagc cgagagagcc
     5221 tgtagacaaa caaaaaacgg cctcccggta gggaggtcgc tcgcttcagg cttggagagc
     5281 agaccctggt tgctgcgcgg cgataaactg tcgactggct actgaccacg ggtcactgaa
     5341 aactcgccag ccggcgcccg cattgctcag tggtagccgt acaaagccga cacgtatacg
     5401 ctttcaattc gacttacgat gttcgtccaa tcgtagtggc gcagcgcgaa gttgcggcac
     5461 tgcttcatca gctcgcgacg ccgggcttcc tgcgacgtgt agcgcagcag cgtgctcacc
     5521 tggtcgatca tgtccggtat caggtgctga gggccgtagc gcaggctcgc cagcctgccg
     5581 atcggatcga gctctgccga gacccgatcc agaaccggtg ccaggccgcc gacgtgcggt
     5641 gcgacgaaca tcagccccgt ggctagggct tcgacgctga cgagggggaa tgcctctttg
     5701 atgagcgacg ggatcacgac gagatcgaca tgcgagatca ggcgcgcgac gtcgggcgcg
     5761 ggttgatacc cggtgaagac gacgcgcttg cgcaggtcgg cggccgccgc cattttgtgg
     5821 tattgatgca agtccagacc ggcccaatag tccgcaaccc attcggcgta cggctccggg
     5881 ctggtctcag ccgcgtgctg catcgcccgg gctgcggcat ccagatcgcc ctccgacagt
     5941 gcatcccgca tcgcgacgag gtgagcgcgg gcaacgccgt cgccgatcaa tatgaaccgg
     6001 gcctggggca ccttggcgac gatggcgggc gcggcggcga tcaggcagtg cacgcccttc
     6061 tcgagcgata gcttgccgat gaaggcgacc cactgctcgc gcgcggtctc gccgctcgtc
     6121 acggctgcca ggtgaggccg aaagtgcacc tgtgtgcggg gcgcctggac agtttggggc
     6181 atcatgagcg caattctccg gatgactgcg actgccgttc cagagcttca gtctcctcga
     6241 tcgttatcat acggtgcgaa ttgggacggt taagttggat gatcggtgat ggatggattg
     6301 gtatcagttg ggaggttgtg tactttttgc aaactcaatg atagcgcctg acactgattc
     6361 tcgatgtgtc actgcgtccc cgtggcaaat cttcgccaga aaggcaagaa gtcgaatagg
     6421 caggtgctaa ggagatggat tgggcgtcag tgtaggtgta ggcagagccg gcatccagtg
     6481 aaaggtgcgc gacgaggagg ggctgatcac ggcgtaaagc ggcgcgccat cgctgccgac
     6541 gccaacctgg tgcatgatcg ttaccgtcca tcggaatgtc cgatgcggat catccggcgc
     6601 gggaaatagc tctagcggaa cgtgaagtgc ggtcgctttg atgtagaccg ggccgtatcg
     6661 aatgtcgccg tcgacctgtt cgagcatcac gcgataggtt tcgtcttcgt ccagaattcc
     6721 ggccgacaac cactgcagca gcacattcct ctcgtttccg gtgatgcggc tgccgttcaa
     6781 gggttggagt agtgtgaccg gcccgtagtt cggctgaggc gtctcggcga ccgatcccgg
     6841 cgtgactgtg gtcgcaccgg cctgtgggat gatgagctgg tcgccgactt gaatctggtt
     6901 ggcatcctcg atctggttga cctgcagaat agccgaaacg gtgactccat attcttgcgc
     6961 gatgccaatc agtgtctcgc ccgcttgcac gacatgaacg atagcggtcg acccggagtc
     7021 tggcgcggct gtcgcatctt gcggcaccag gctcggcgtc ggcgtcgcgg tcggaggcgg
     7081 gatcaggatc ttctggctga cctgaaggac ggagttctcc gtcaggttgt tgaacgcgag
     7141 aagatcacgg tagtcgacct gaaaaatgtc ggcgatgtag aagatgttgt cgccgggttg
     7201 gacgacgtat tctcgcggcg ggatcggcgt cggcgtgaac agcggcgtcg gcgtaggggt
     7261 tggggtgcgt gagggcgtcg gcgtggccgt cggcgtcggc gtagccgtcg gcgtcagcgc
     7321 ggcgatggcc tcggcccgat tgcgaaaata gagcgccgca atcaagagca gagcgactgc
     7381 ggctgccacg ccgatggcaa tggcccgaac cggtcgctgc gccgcctctt cgcgcagctc
     7441 ggcctgctcg gcccgctcgg cctgctcggc ggcgacgaag tcgtagccgc agatcgcgca
     7501 ggtggttgcg cgcgtcgaca ccggcgtgca gcactccgga cacagccggg atttctcatg
     7561 tgttacgggc gcttcgggtt ctgcgcgctc tcgacccggc agcttcaccg agcgctcccg
     7621 tccagcccca gcgccggcgc tgcttcttgc atcgctgcca gcacgacgcc gatgtgctcg
     7681 tcgagcggga cacccaggtc ggccgcggcc cgctcgatct cctcgcggtg cacgcccgcg
     7741 gcgaaggctc tatctttcca cttcttcttg acactcgaga cttgcacgtc ggcgatgttc
     7801 ttgcccggac gcacgagcgt gacggctgtg atcaggcccg tcaattcgtc aactgcgtag
     7861 agcgcctttt ccatcagagt gcgccgctcg acgcgggtga cctcggtggc gtgtgacagg
     7921 atgcagcgga cgatctcgtc cggccagccc atttcgcgga gcactcgtga tccgacgacg
     7981 gggtggccct cggcggcgac gtccggatag cgctcgtagt cgaaatcgtg gagcacgccg
     8041 accgtcgccc acttctcttc gtcctcgccg aagcggcgcg cgtaggcgcg catgccggcc
     8101 tcgacggcca gcatgtgttt gcgcaggctg tcgctctgcg tccattcggt gaccagtatc
     8161 cacgcggcgt cgcggttcat tgggtcgttc caggggttat cgcggcggga ttataccatg
     8221 ccgttccggc catgcccgtg ctttgaggat attggaaatc agagattgga gattggaatc
     8281 tcggatctcc ggtcgccgtg actttcgagt aggctacatg agcgacttga tctgggcttc
     8341 gagcttggcg cgtttgtttt gaagagcggc gagtttatcg cgctccttct ggacgacggc
     8401 ggctggcgcg cgcgtgacaa aatcgcctgt cagcagcgcc gtgctgcgag tgatctgcgc
     8461 ctcggcgtcg gcaaactctc tgttgagccg cgatcgctcc gtgtccaggt cgaccatttg
     8521 cgccagcggc aggtataccg tcgccgaagc catcaccagc gataaggctc tgtcgggtat
     8581 cgctttcaat ccgggcgcga tatgcaggcc gtcggcgtcc aggcgcgcga gtagggccat
     8641 cacatcgcgg tgtgcctcga acaggttcgc tttgtcgccc gaaatcagca tggccgccag
     8701 ccggttctcc ggcttcacgt cgtgctcggc gcgcgcgttg cggatagcgc ggatggcctc
     8761 catcaggacg cccatgtcgc gctcggcagc ctcgtccgtc gcgccaggct cgggccaagg
     8821 cgcgacgatc agcgcggcgg gccagttctt gtcgccggcg gccttcttca ggtgctgcca
     8881 gacttcctcg gtgacgaagg gcaggaatgg gtgcagcagg cgcagcgcct ggtcgagcac
     8941 gtggacgagg atcgagagcg ttcgctttcg agcctcggcg tcgccgctac gcagcggcag
     9001 cttggatgcc tcgatgtacc agtcggcaaa ctcactccac aggaactcgt ggatgagacg
     9061 cccggcctcg ccgagctgat agtcgccaaa caggttcgtc acgtcggcga tgagccggtt
     9121 gagacgggag aggatccagc gatcgggtaa ttggagattg gagattggag attggaggtt
     9181 agaaaggcct tttccgactt ccaacctcca acttccaaca tccagatgcc caattacaaa
     9241 tcgggcggcg ttccagatct tgttcgcgaa gttacggttg gcttccgcgc gctgtgagct
     9301 gaagttcatg tcgttgccag gcgtcgagcc ggtgagcagt gtgaggcgta gcgcgtcggt
     9361 gccgtactgg tccatgatgg tcgtcggatc gatcgtgttg ccaagcgtct tgctcatctt
     9421 gcgcccgtgc tcgtcgcgga tcaagccgtg caggtacacc gtgtggaacg gcggcttgcc
     9481 ggtcatctcc aggccgagca tgatcatgcg cgccacccag aagaacagaa tgtcgtagcc
     9541 ggtctccatc acgctggtgg gatagaaatc cttatagtcc ggcgtctcgt ccggccagcc
     9601 cagcgtcgag aacggccaca agcccgacga gaaccacgtg tcgagcacgt ccgggtcttg
     9661 ctcgatgttg tggcggccgc atttggcgca cgaatacggg tcctctcggg cggcccactc
     9721 gtgcctgcag tccttgcacg tccacaccgg gatgcgatgt ccccaccaca actggcggct
     9781 gatgcaccag tcgcggaggc gcgaagcgtc cagccagttg aaatacacct tggcgaagcg
     9841 ctccgggata atgcggatct tgccggagcg tacggcttcc agggcaggct cggccagcgg
     9901 ctggatcttc acgtaccact gcgtcgagat gagcggctcg atgatctcgc ctccccgctg
     9961 cgagcgcggc acggtcatcg ggtacggctc ggtcttaatc gccagcccgg ccgcttccat
    10021 gtccgcccac atccgcttgc gacactcgaa gcgatccagg ccctggtatg gcccggcgtt
    10081 ctcgttcatc gtcgcatcgg ggttcatcac gctgacaatc ggcagcccgt ggcgctggcc
    10141 gatctcgtag tcagtcagat cgtggccggg cgtgatcttc agggcgccgg tagcgaaccc
    10201 cggatcgacc attgtgtcgt ggatcacggg gatggtgcgg ttgaggattg gcaccagcac
    10261 agtctcgccg atcagatggc ggtagcgctc gtcgtcggga tgcacggcga cggccgtatc
    10321 gcccaggatc gtctccgggc gcgtcgtcgc gacggggatg tacgcgtcgc gatgcccggc
    10381 caggcggtac ttgaagtagt ataaggtgcc ctgctcctcg ctgtattcta cctcgaggtc
    10441 ggacaccgcg gttttcaggc cgggcgacca gttcaccagg tacgtgccgc gatagattag
    10501 ccccttctcg tagagccgca cgaaggcttc gcgcaccgcg cggctgagac ccttgtcgag
    10561 tgtgaaacgc tcgcgatccc agtcagcgga aacgccgagc cgcttgtgct gctcggcgat
    10621 gtacgaaccg tacttctcct tccacgccca cgtgcgccgg atgaactcct cgcgcccgag
    10681 ctgctcgcgt gtcacgcctt ccgtgcggag gatgtgcttc tccacctgta actgtgtggc
    10741 gatgccggcg tgatccgtac cgggcagcca cagcgtcctg tcgccgcgca tgcggtggta
    10801 gcggatcagg cagtcttcga ccgaagcagt catcgcgtgt ccgatgtgca gctcgccggt
    10861 gacgttcggc ggcggcatcg agatgacgaa tctgtccgcg tggcgtctgc gagcgatctc
    10921 cggcttgaac cagccgttct tctcccacca agcgtacagc cgctgctcgg tcgtcttgaa
    10981 gtcgtaggtc ttgggcatgc tgtgttcggc catgttcccc tccggttagg gttgagagtt
    11041 agagttgaga gttagagtta gagttagagt tgggagcccg tcggaaaaca aaagtccctt
    11101 tcgtctcaag gacgaaaggg acaagccccc gtggtaccac cttggttcgg cctcggatgc
    11161 atcgaattgg caacggataa acggatgcag cagttcgtat ccgtttatcc gatgtacatc
    11221 cgttgccgca ctcgttcgct gtaacgggct tgcccggatc gacttactcg cctgagaccc
    11281 gtcaggtctc cgagacctga cgggtcttac catttcggtc gatcaactcc cgagcgacgt
    11341 tcggcgtctt gagccgacgg aggctttcag ccggtgaccc ccgatcgctg gcgacgtcag
    11401 gcgcctactc ctctcgttcg ccgtcgttat gagcggcatt ctatcaccgc ttggacagcg
    11461 cgtcaacccg ctacttccac ccgaaagaac tcgtgcgcgc ctgcctcgac cgcgttcatc
    11521 agttgcacga accacacctg gaactcgggc cgcagaaaca tcgagcggcg gtgcgcttcc
    11581 cagtcggcca gattgtctac ctccacctcg atcaccgcct ggaacatcgg cccactcacg
    11641 tcggtcgtga tgcgcggttt gcggccgacg cccgaatcga ccatcagcgc gccataacgc
    11701 ctgagcagat cgaccgcctg gcgaatctgc cactggcggg cacgatagtt ctggcgcacg
    11761 acgatgacgc ccgtccgcga ccagtcttcg cactctccct cgaccgtgta aaactcctgg
    11821 cggccattgt cgatgaacaa ctggaagccc ttgaaccagt tggcaaattc gggatgattg
    11881 aacagccgct cccggctcgc ttcccactcg gtgagcgatg gcagcacgaa ttcctcggcc
    11941 aacgtgtaca tcggaccaga gatgtcgctc aacagatggt agtgggccgt cgcggccacc
    12001 tcctcaggca aatagctcgt cagccgcctg aacagctcga cagcctgatc gatcttgccg
    12061 aatttgacct ggtagacggt gcggacttcg atcatatatt ccctccgaag tgatggtcac
    12121 aggttgaaca actgcttgag ccattccatg ccgtaggccg ccgcgtaggc gacggcgacg
    12181 ttcttgatga tcttgcccgc acttgccgag aggagataca gccacaccgg catgcgcagc
    12241 gcgccggcga tgatgccggc cacgtcaaag agcgcgatgg gcagcagcgc cagcacgaag
    12301 atgatcggcg cgccgaagcg gcgcatccag ctcaccagcc gggcatagcg cgggctgtcg
    12361 tcgatgagcg tttgcccgct gtagccggcc atgtagccgg tcatctcgcc gagtgtctgt
    12421 cccgctcccg cgacgatgcc gacgacgaat gggttggcga ccgacccggc gatcgccgtc
    12481 aacgccaggc cgggcacggg cagcaccagc gtcgcgctcg tgatcgcccc gaagacgaac
    12541 aacaccacca gcccgctggg cgacgtgatc agttcctcca cccgcctgag cctgcccggg
    12601 tcggacacga tgtacagcgt gccagccact gcgccgatga gaatcaggac ggtcaggata
    12661 cgaaccaggc gcaagcgcgt tctggagggc ggcggaggca cggcttcggc cagcacaggc
    12721 ggggcctcca caggtgtgtc ggcgttcatg cgctcctgtt cgtcgcgagc aactcacgac
    12781 tccacaatgt ctatgcgcgt catagccagc ccaggctcga tcgctctggc tggatcgcgc
    12841 tcaggaatct tgagcacgac gtccattccc tgcgtcactc tgccgaagac gctgtgccgg
    12901 ttgtcgagat gcggcgtggg gccgtgcgtg atgaagaact gcgagccgtt cgtgttcggc
    12961 ccggcgttcg ccatcgatag aatgcccggc gcatcgtgac ggagctgggg atggaactcg
    13021 tcgccgaacg tgtagcccgg gccgccgcgg ccggtgccgg tcgggtcgcc gccctgcgcc
    13081 atgaagccct ggatgacgcg atgaaacgtc acgccggcgt agtagccgtc gcgtgccagg
    13141 aatacgaaat tgttgacggt ccgcggcact ttatcggcga acaactcgat gacgacatcg
    13201 ccgatcggcg tcacgatcgt cgccgtgtac ttcttctgcg gatcgatgac catcggcggc
    13261 ggagctgagt atcgctttgc catgcgagtc tccttaggtg tttgacgaat gcgaattcct
    13321 tctgttcctt gcgatgcatg gaacggtctg gaaggaactg aaggaactac ggcgcgccga
    13381 gacgaacaat ggttgcctcc aggtcacgcg gcacgacgtt gcacgtatcg acgagcaggc
    13441 gggcgcgccc ggcgacgcgg cggtagtcga ccgcgctgtg gccggtcacg accacgatgc
    13501 agtctgcaga ggacacggcg tcgtcggtca acgggatgga cgcgagccgc tcccgctgcc
    13561 ggtgaaacac gttcccgccg gcgtgaaact ccggcacgtg cggatcatga tagcacaccg
    13621 ctgcgccgcg cgccatcagc agctccatca cccgctcggc gggcgagttg cgcgcgtcgt
    13681 cgatgtcgcg cttgaacgcc acgcccagca ccagcacgct cgcgccgtcg agcgcgcggc
    13741 cttgtttgga gaggccgcgc acgatcaggt cgacgacgtg gtacggcatg tcgtcgttca
    13801 cctgcgccgc caggtcgatg aagttggtgt ggaaatcgaa ctcgcgcgcc ttccacgaca
    13861 gatacagcgg atcgaccgga atgcagtggc cgcccacgcc cggccccggc cggaacggca
    13921 taaagccaaa gggcttggtg gccgccgcgg cgatcacttc ccacacgtcg atgcccatgc
    13981 gctcgcacaa cagcgcaagc tcgttgacaa gcgcgatatt gacgctgcgg aaagtgttct
    14041 ccagcagctt agatagctcg gccgcgcgcg gcgacgaaac gacgtgcacc ggcgcgccca
    14101 tcgtctccag cagcgccgcc gtgcgccgcg caccctgcgg cgtgagcgcg ccgacgactt
    14161 tcggcgtgtt cttgatcgtc cagcccttgc tctctacctg tccgg
//
LOCUS       DBNHXB010000397         3966 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2294757, whole genome shotgun sequence.
ACCESSION   DBNHXB010000397 DBNHXB010000000
VERSION     DBNHXB010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2294757"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="AC162G_15995"
     CDS             complement(<1..1154)
                     /locus_tag="AC162G_15995"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5077358.1"
                     /translation="MTLDKRNLYRFPWSKNDNPIGWLEVTDICNIHCLGCYRQKLTGH
                     KSLDQIKDEVLFFKRWRNCDNISIAGGEPLLHPHIMDTVAFIAQNGMKPILLTNAIKL
                     DRPTIVELKKAGLAGFTIHIDSFQGRPRWKDKDEVETNELRQHYADLIHDVGGLYLIL
                     NGTVYPSTFDYIPEVVRWGQRNVDRVHGLVFITFRVAPLDEEVTYQRGDEFVDARHLS
                     YTSETPPERNITSYEVHDLIKEHFAEFDASGYLGGTWKVDSIKWLAGAMIGTRRRMYG
                     SIGPKTMELAQAGHHFFKGRYLAYLNQSKVGRKVFWLAAIDGRVRAAYRRYLGDLLRH
                     PLRVFDSVYIQSIGIIQGPDIMPDGAADMCDSCPDMTVWNGELINSCRMD"
     gene            complement(1282..2166)
                     /locus_tag="AC162G_16000"
     CDS             complement(1282..2166)
                     /locus_tag="AC162G_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR5077359.1"
                     /translation="MRYFTGSAIWAYQGWVGNFFPKGTKQGDFLREYARRLTAVEGNT
                     TFYAAPSAATVERWRDDTPQGFRFCPKLPRSVSHAGQLAPHLKEALNFVDLMRGLGSR
                     LGPLFLQLPPRYSPKFINDLRAFLKAWPRDLKLAVEVRHLDWFGEHHGELNALLREHD
                     AARVLIDTRPIREMPEAEIGRGSVYMRMVERLEQARERKPDVPLLPERTASFALVRYI
                     GHPRIDLNAPLLDEWADRFAGWLSEGADVYAFCHCPDEIQSPLICRELHRRVAARARI
                     DPLPWDEQEHTLEQGRLF"
     gene            complement(2170..3117)
                     /locus_tag="AC162G_16005"
     CDS             complement(2170..3117)
                     /locus_tag="AC162G_16005"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5077360.1"
                     /translation="MNAPSTLRFGRTDLIVMAVVAIWGVNLSVVKSAISGPGAPFTPL
                     AFNALRFCVASTALFFMLRRAGDAVPTSRRDWMAVLGIGLLGNTVYQALFITGIDHTS
                     PANSALIAAATPVIVALIGAALRVERLGMLAWIGVVMSFAGIAVVVLGNASVASGGPG
                     ASSLVGDLFILMAAIVWAVYTLLVASLLRRYSAMAVTALSVAAGTVLIIIMALPDLSR
                     LDWDAVPSEAWSAAVFSGLFALAVGYAGWNRGVKAIGSARTAVYSNMIPIAAALFAWL
                     GRGDPITVYHVAGAVIVLTGINLTRLGRQVRVDTLPVEE"
     gene            complement(3149..3751)
                     /locus_tag="AC162G_16010"
     CDS             complement(3149..3751)
                     /locus_tag="AC162G_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077361.1"
                     /translation="MELRYVGTVARLQIQRSSLKVGQKPHRVYDTSPLLSVEQISVSP
                     DGAIALTPDGQQTLDVHHRLHDKSQFAGVNGLSIGFTSHYVRMRARFGDHMFHGCAGE
                     NVLIDTDALPGLSDVERGVLIRCAAMGVDLWLGDVRVALPCLEFSRFVLRLPQVERES
                     SEVKRALQFLEQGTRGFYVTPANPGAPVVVSVGDRVYFPL"
BASE COUNT          796 a         1247 c         1234 g          689 t
ORIGIN      
        1 tcgtccatgc ggcacgagtt gatcagctcg ccgttccaca ccgtcatgtc gggacaactg
       61 tcgcacatgt cggccgcgcc gtcgggcatg atgtccggcc cctggatgat gccgatgctc
      121 tgaatgtaaa cggaatcaaa tacgcgcaac gggtggcgca gcaagtcgcc caggtaacga
      181 cgataggccg cgcgcaccct cccgtcgatc gccgccagcc agaacacttt gcggccgacc
      241 ttcgactggt tcaggtaagc caggtatcgg cccttgaaga agtgatgccc ggcctgcgcc
      301 agctccatcg tcttcgggcc gatcgacccg tacatgcgcc gtcgcgtgcc gatcatcgcg
      361 ccggccagcc acttgatcga atcgactttc cacgtgccgc ccaggtagcc cgacgcgtcg
      421 aactccgcga aatgctcttt gatgaggtcg tgaacttcgt acgaggtgat gttgcgctcg
      481 ggcggcgttt cgctggtgta gctcaggtgg cgcgcatcga cgaactcatc tccgcgctgg
      541 taggtgacct cctcgtccaa cggcgcaacg cggaaagtga tgaacaccag gccgtgcacc
      601 ctgtcgacgt tgcgctgccc ccagcgcacg acctcgggga tgtagtcgaa cgtcgaaggg
      661 tagacggtgc cattcaagat cagatacagt cccccgacgt cgtggatcaa gtcggcatag
      721 tgctggcgca gctcgttcgt ctcgacttcg tccttgtctt tccagcgggg ccggccctgg
      781 aagctgtcga tatgaatggt gaacccggcc aggccggcct tcttcagctc gacgatcgtc
      841 gggcgatcga gcttgatcgc attcgtgagc aggatgggct tcatgccatt ttgcgcgatg
      901 aaggccaccg tgtccatgat gtgcgggtgc agaagcggct caccgcccgc aatcgagatg
      961 ttgtcgcagt tacgccagcg cttgaaaaac agcacctcgt ctttgatctg atcgagcgac
     1021 ttgtgaccgg tgagcttctg ccggtagcag cccagacagt ggatgttgca gatgtcggtc
     1081 acctccagcc agccaatggg attgtcgttc ttggaccagg ggaagcgata aaggttgcgc
     1141 ttgtcgagtg tcatatgtgg ctccatgtca ggggtagtta cattgacaac actcgacggt
     1201 gaaaagcgtg acgcacagaa gagttattca gaatatgatg aatattaacg tggcaatgtc
     1261 agattcggct cgccgcgcac ttcaaaacag gcggccctgc tcgagtgtgt gctcttgttc
     1321 gtcccacggc agtggatcga tcctggctcg cgccgccacg cgtcgatgta gctcgcggca
     1381 gatgagcggc gactggatct cgtcggggca gtggcagaag gcatacacgt ccgcaccttc
     1441 gcttaaccag cccgcgaacc gatcggccca ttcgtccaac agcggcgcat tgagatcgat
     1501 ccggggatgt ccgatgtagc gcaccagggc gaacgaggcg gtgcgctcag gcagcagcgg
     1561 cacgtcaggc ttgcgctcgc gcgcctgttc caggcgctcg accatgcgca tgtacacgct
     1621 gccccgtccg atttcggctt cgggcatttc gcggattggg cgcgtgtcga tcaatacgcg
     1681 ggcggcgtcg tgctcgcgca gcagggcgtt caactcgcca tgatgctcac cgaaccagtc
     1741 gaggtggcgc acctcgacgg cgagtttcag gtcgcgcggc cacgccttga ggaacgcgcg
     1801 caggtcgttg atgaacttcg gcgaatagcg tggtggtagt tgcagaaaca gcgggccaag
     1861 ccggctgcct agcccgcgca tgagatcgac gaagttgagt gcctccttga gatgcggcgc
     1921 gagctgcccg gcgtggctga ccgagcgcgg cagctttggg cagaagcgaa agccttgagg
     1981 cgtatcgtca cgccaacgtt ctacggtggc tgccgaggga gcggcgtaaa aagtggtgtt
     2041 gccctcaacg gcagtcaatc gacgggcgta ctcgcgcagg aagtcgcctt gcttcgtgcc
     2101 ctttggaaag aagttgccga cccaaccctg gtaggcccaa atggcagaac cggtgaagta
     2161 gcgcatttat cactcctcta caggcagcgt atctacccgc acctgcctac ccagccgggt
     2221 aaggttgatt ccggtcagca caatcaccgc gccggccacg tgatacaccg tgattggatc
     2281 gccacggccc agccaggcaa acagcgccgc cgcgatcggg atcatgttag agtacaccgc
     2341 cgtccgcgcg ctgccgattg ccttgacacc acggttccag ccggcatacc cgacggcgag
     2401 cgcgaacagg ccggaaaaca ccgccgcgct ccaggcctcg gacggcacag catcccagtc
     2461 gagccggctc aggtcaggca atgccatgat gataatcaac accgttcccg ccgcaaccga
     2521 aagagccgtg acggccatcg cggaatagcg gcgcaggaga gacgcgacga gcagcgtgta
     2581 gaccgcccat acgatagcgg ccatcaggat gaacaggtct ccaaccaggg acgatgcccc
     2641 cggcccacca gacgcgacag acgcgttgcc gagtacgacg acggcgatcc cggcaaaaga
     2701 catgacgacg ccgatccagg ccagcatccc caacctctcg acgcgcaggg ctgcgccgat
     2761 cagcgcgacg atcaccggcg tcgccgccgc gatcagcgcc gagttggccg gcgacgtatg
     2821 atcgatgccg gtaatgaaca acgcctggta gaccgtgttg cctaacaggc cgatcccgag
     2881 gaccgccatc cagtcgcggc gtgaggtcgg gacggcgtcc cctgcccggc gtagcatgaa
     2941 gaacagcgcc gtcgacgcga cgcagaaccg cagcgcattg aacgcgagcg gcgtaaacgg
     3001 cgcgcccgga ccgctgatcg cgctcttgac gacgctcaga ttgacgcccc agatagcgac
     3061 caccgccatc acgatgaggt cggtgcggcc aaaccggagc gttgacggcg cattcatttg
     3121 gctcatggtg cgcctggctg cgcgggcgtc agagaggaaa atagacccgg tcgccgaccg
     3181 agacgaccac cggcgcaccg ggattggcag gcgtgacgta aaagccgcgt gtgccctgct
     3241 ccaggaactg gagcgcgcgc ttgacttccg agctttcccg ctcgacctgc ggcagacgca
     3301 gcacaaatcg gctgaactcg aggcacggga gagcgacacg cacgtcaccc agccacaagt
     3361 cgacgcccat cgcggcgcag cgaatcagca cgccgcgctc cacgtcgctc agacccggca
     3421 gcgcgtctgt atcgatcaag acgttctccc cggcgcagcc atggaacatg tgatcgccga
     3481 agcgcgcgcg catgcgtacg taatgcgacg tgaatccgat cgacaggccg ttcacgcctg
     3541 cgaactgcga cttgtcgtga aggcgatgat gcacgtcaag tgtttgttga ccgtccggtg
     3601 tcagagcaat cgccccatcg ggcgacacgc ttatctgctc gacgctcagc agtggcgagg
     3661 tgtcgtacac gcgatgaggt ttctgcccga ccttcaacga gctgcgctga atctgcaagc
     3721 gggcaaccgt tccgacgtaa cgaagttcca tggatgctcc cagataggct ccagcgtggc
     3781 aggatactac cacagataca agaggcgcgt tatctgggct tagcgccaga cgacgtccga
     3841 agcgctcagg gcaactcgta aatcacgacg ccgttggcgt cgaggcgttt gcgcacgccg
     3901 atcgcttcgg ctgcatccag cgcgtcgggg tacaggccca cgcgctcggt gacgacgacg
     3961 aagcgc
//
LOCUS       DBNHXB010000398        14423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4642667, whole genome shotgun sequence.
ACCESSION   DBNHXB010000398 DBNHXB010000000
VERSION     DBNHXB010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 14423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14423
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4642667"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="AC162G_16015"
     CDS             <1..1017
                     /locus_tag="AC162G_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR5077362.1"
                     /translation="QLEGVIRTLQADGYPLVGAHNDTVVVDTADGEVNNKHKFVTDQY
                     GVPCIYLYKPDVKWITYTPKEPFRVLGDVYPEGVDIPEPFIGTNIVHLPTIKTHVFTT
                     ITGAMKNAFGGLLHRKRHWTHAVIHDTLVDLLQIQQDIHEGLFAVMDGTFTGDGPGPR
                     AMRWHEKDILLASADQVAIDAISAKLQGFDPMSIRFIRRAHEDGLGIGDPKQIKVVGY
                     DIEQEPAWGFVQEDTFASRGQKLIYHGALKPLEGLLLRSPIVPWSYFASNFYHNVYWY
                     PFVGRPRVRAALNTKWGRLFARYGDGKVVMPGMQPETVAIAAAGAALAGGMIAAGWML
                     RRRR"
     gene            complement(1052..1894)
                     /locus_tag="AC162G_16020"
     CDS             complement(1052..1894)
                     /locus_tag="AC162G_16020"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR5077363.1"
                     /translation="MTVAFVLSGGGNYGSLQVGALQVLLERGIRPDLLVGASAGAMNA
                     TFLAMDPTLEGLHRLAEHWRRAAHEAVSRRGNLAMIWRLVTRRDGLTPNAPLQRYLER
                     TLPPGVVCFGHLRLPTYALAVRLDTGEVRCFGDDPSDRIIDGLMATTAIPGFYPPWEC
                     DGARYVDGGVVSFLPVQMAVERGATEIYALNVSPASTRTDGLRGTLAISLRAIDLLIQ
                     GQGAREIQVYESLYGVTVHHIRLSSEHPIAFWDFRHADHLMAAGRRRTEAYLDGGGVM
                     TKGD"
     gene            2110..2442
                     /locus_tag="AC162G_16025"
     CDS             2110..2442
                     /locus_tag="AC162G_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077364.1"
                     /translation="MGIRNIGRKTVGEVRAEIKRGARFVVYEYVISLLVVTRRRASPV
                     YYIEPGRSPAHFHWPYSLLTLALGWWGFPWGPAFTIGAVRDNLRGGHDVTAKMLETIL
                     AAADKKRK"
     gene            2442..3413
                     /locus_tag="AC162G_16030"
     CDS             2442..3413
                     /locus_tag="AC162G_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR5077365.1"
                     /translation="MNVICVATRKGLFVFQRDGGDWREVAAGLGGHNVTSVIAREGVI
                     LAGTREGVFRSDDMGRTWREINNGLSVRYVRWMAYHPDVSDLEFAGTEPAGIFVSRDG
                     GDSWRECPEVAQVRDAHGWSLPYSPRAGCVRGLAFHGARAYAACEVGGVLRSDDGGET
                     WRLAAGSSGDPEPYYPPAPRVCPDVHSIAIHPSSPDLVFAPTGGGLYRSSDGGKTWTS
                     LYDCYCRDVWLDPRDPDHIVFGPADFVDSNGRIEESRDGGRTWTSAGTGLKTPWRRHL
                     VERFAQIGDELLAVLSNGELIAALLATLGWRRILPDVTGVECVTAMR"
     gene            3692..4450
                     /locus_tag="AC162G_16035"
     CDS             3692..4450
                     /locus_tag="AC162G_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR5077366.1"
                     /translation="MSGHSHWATIKRKKGANDARRGQTFSKLGREIAIAARDGPDPDN
                     NVKLRLIVNKAKAAGMPKDMIERTIRRGAGLDKDAAAFEELMYEGYGPHGIAMMVKVT
                     TDNRNRTVSDIRRIFTRSGGSLAESGAVAWNFEQKGYISIPIANPDPDRLFDIAVEAG
                     ADDVEITEDHLEIYTAPNELHAVNSALEKAGLKAETVELTMKPRQLTPLSMSDAQSVL
                     QVVDLLEELDDVQQVYASLDLPEELVTGYEPQAA"
     gene            4544..5041
                     /gene="ruvC"
                     /locus_tag="AC162G_16040"
     CDS             4544..5041
                     /gene="ruvC"
                     /locus_tag="AC162G_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR5077367.1"
                     /translation="MIFLGIDPGTAITGYGFIREEGSSLRAVAYGVITTPPDLALPQR
                     LVLLHRALGDLIRLHRPASAAVEQIFFSRNARTALAVGHARGVVLLALAQAGLPVAEY
                     TPLEVKQAITGYGRADKKQIQEMVRVLIGLPEVPRPDDAADALAIAICHLHSSRIRDL
                     AGGAP"
     gene            5038..5619
                     /gene="ruvA"
                     /locus_tag="AC162G_16045"
     CDS             5038..5619
                     /gene="ruvA"
                     /locus_tag="AC162G_16045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR5077368.1"
                     /translation="MIARLRGTVESIDKDGLIVSVGDVGFHVRASAAARELAGRSRSV
                     DLFTHMHVRENEIALYGFASEEELALFELLLGVSGIGPRVALSILAGAPPERLRQAIA
                     AEEIDVLTRVPGVGAKTAKAVILHLKDKIGAVPGVPWPAAFADDDVEIVGALTALGYS
                     IAEAQAAMASVPRGSGASVEERLRLALAYFARP"
     gene            6128..8377
                     /locus_tag="AC162G_16050"
     CDS             6128..8377
                     /locus_tag="AC162G_16050"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5077369.1"
                     /translation="MNQLANPQTATTARRPLNLGIRGKVTIPYLVLTFAVAFVGVFIV
                     TRYVTDSFAERFANQLADAARVAGEGFVGIEQRHLDMWRILAATEGVQDAVAARDLPT
                     IRALVDGPARSRSVDSVWIVDLQPIVLVGLDRTPNGDYTEANNFPFGEFEPLERALRG
                     ESDEFGDKFAGIQFRSGQPYLVTAGPLEDGGKLFGAILIGTRLDRALRSIDQQAAARI
                     SAYDTNGDLLGTTLLGEGGPPDQNRLPPDQINEIFARAAAPEYLSGDLQHGSTPYRLL
                     YAPLQIRQSVIGAYSVGLPSDFIFLKSADSRLLFAFIFTAMALGVVVVGFLLSRRLIA
                     PVLQLVRTSQAVASGDLNQRTGLAGGDEIGLLANTFDDMTYKLQERTRDLELLLRAHR
                     EEAFKIRAILSSIADGVLVLDPQGRIIMMNAAAEHILGDLSADFMTGTFREQPMNPIE
                     PPPESMALIDSAEVRRFELNNRTISTHAAPVIIPEGHQLGTVIVLRDVTREAEIDRLK
                     DHFIEQMSHELRTPLTAVKGYSDLILQTGGSEIPEKYLEFLNIINRHADSLVAMITEL
                     LDISQMEAGAMSLRRERIDLNELVDVALSEWRRRFAEKELALEVNLAPEPVAITGDRR
                     RLNWAIRQLVSNAYHYTEPGGRATVSVAGDAEAVSISVQDTGTGITPEDQKFLFTRFF
                     RSTTRVHSNERGVGLGLYIVKAVAEAHHGKVEVHSVVGKGSTFRLLLPIEEVDDELPV
                     VKMPQDART"
     gene            8374..9939
                     /locus_tag="AC162G_16055"
     CDS             8374..9939
                     /locus_tag="AC162G_16055"
                     /inference="COORDINATES: protein motif:HMM:NF012271.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR5077370.1"
                     /translation="MSRQPRREPIAARLPYVLMILAGLACIVLIALSSVGQQGIGWLV
                     VPAGLQPNSTADYSLQVGATLPAVDAEQVAMLLATDEAGLRVTPKPGTIITVPAGQFT
                     PGPTDVAQVTATPRATRTPTRTPPSTRTPTATPSRTRTFTPTSTRVRTATPTFIPSPT
                     PVIPTATNTSAPPPPPPPPPPPPNTATWTPTSPPSVTPSPTNTPSSTNAATLTYTPSP
                     TSVTPTNTPSITPTVNPPPPAPSSLAVVNTSSSLISLVWSNESALDADFVGYNVYIST
                     VPGGPYSFVASAGTATSYVVSGLSSGTTYYFVVRGADTVQESGNSPEASGTTGAVSNP
                     YPTPPPSCPPPAPPDCGNAGGPPDGNWSYVAPGQELILDLGANNGILDGPGWDFVYYE
                     RETGTTGSIGMDWVTIELSSDQVTWYVAFTWNAGSNAQNSNIAPFASPTPDPNYCDLW
                     AGAASDEPIPMGACGISWGGLWGTPPTPQTGIAIDIGPVIPTIPPEGFRYIRIQGIDP
                     AQPAEIDGIERLN"
     gene            10254..10463
                     /locus_tag="AC162G_16060"
     CDS             10254..10463
                     /locus_tag="AC162G_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077371.1"
                     /translation="MAQVSLDEARVKELFKQAMLELFQEQRGLLYDLFVEVLEDLALV
                     NAIQEGESSETVSRAEVFQILESAA"
     gene            10460..10726
                     /locus_tag="AC162G_16065"
     CDS             10460..10726
                     /locus_tag="AC162G_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002755356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
                     /protein_id="HIR5077372.1"
                     /translation="MKVEFKSSFAKDLKKVKDKDLKARVKQVIELVEKAKSLQEIQGT
                     KKLKGGDRYYRIRIEDYRVGLTLEGDTVTFVRFLHRKDIYRYFP"
     gene            complement(10873..12513)
                     /locus_tag="AC162G_16070"
     CDS             complement(10873..12513)
                     /locus_tag="AC162G_16070"
                     /inference="COORDINATES: protein motif:HMM:NF015755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR5077373.1"
                     /translation="MESERSNSPSAAAVEWLLVFVVVGLIVLIGIFLAGSAGKEVAML
                     VATSTATPTTRYSPTPSFTPTETLTPSNTPTPTETLTPSPTPTGTPTPRPPLPGVAGT
                     VTPLPTPEVITDATTLVPTPAPIVALPRDTINVVVLGSDRRPGDSSWRDDTIILISVR
                     QNPTFVTLLSIPRDLWVYVPNWQHNRINVVDAHGERSGFPGGGAGLVKQTIQYNLGIE
                     VQYFVRIDFNAFRAIIDTLGVVREGDDKPTVRVLADCPLTDIFPDVPEGQTDILSGDA
                     LSTTITGTLEIVPGYNYLDSKHALWFARSRLSTNDFDRSRRILNLGLALLQAARENGT
                     LGKVPELWNHLSASIQTDLTLNDVLWLAGIAGELDPSAIKLRQIDAVVTQGFVAENGA
                     QVLHINSEVLPLVLAEAFDPPPANVASQASAAVEVWNASGFPDWGDLAIDRLLRYNFE
                     VIGPPHSDVDLRADTVIVDYTTTSKGSRVSQLARLFQVPAGNIVSQPDPNSPVTYRLI
                     VGAGYDPCRKPPAPVEVTPTPSPTITPTIDPALLTPTP"
     gene            complement(12715..14313)
                     /locus_tag="AC162G_16075"
     CDS             complement(12715..14313)
                     /locus_tag="AC162G_16075"
                     /inference="COORDINATES: protein motif:HMM:NF015755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR5077374.1"
                     /translation="MGTRAASRRSSLSAIDFILIAIIIGLLTVICGVLAMRLLPFANP
                     VILTIATPTITLTPSPTTPPTPSPTWTNTPTPTRTRTPTPTPTRTLPPTSPPPPLVNL
                     ALTVTPLPPVEVITGTFWTPPSPAPLREFKPNTLNIILMGSDRRPNEGGWRTDTMILV
                     SVDPDVPSLTMLSIPRDLWVYVPNWQWTRINLADGRGERIGFPGGGPGLLKQTIQYNF
                     GIPVQYYARVDFGGFKALIDAVGGIDVIADCPLYDIFPDVPEGVTDIITGPELATVVT
                     GTIDIPSAGIYHLDGKHALWYARSRKTTSDFDRSRRQHRVLRGLWSAIQQQGLVGQLP
                     ALWDQLSQAVQTDLTLNDVVYLAQLGTQLDPTHIRSRFIDGTMLHYFVSNNGASVYAF
                     NYDELEPVLDEAFAEFLPNVASQAPAAVEVWNGVGNPDWDRVAAERLSWAGFQAWPNG
                     IADRLYERTTIVDFTTTQKGSRLAQLINLFRVSSADVVYQPEASSPVPYRVILGQNFD
                     PCLRRAPVPIATATPMPASVPAAP"
BASE COUNT         2772 a         4526 c         4595 g         2530 t
ORIGIN      
        1 cagctcgaag gcgtgatccg gaccctgcag gccgacggct atccgctcgt cggcgcgcac
       61 aacgacacgg tcgtcgtcga taccgccgac ggcgaagtca acaacaagca taagttcgtc
      121 accgaccagt acggcgttcc gtgcatttac ctgtacaagc cggacgtcaa atggatcacc
      181 tacacgccga aagaaccctt ccgcgtgctg ggcgacgtgt atcccgaagg tgtggacatc
      241 cccgagccgt tcatcggcac gaacatcgtt catctgccaa ctattaagac tcatgtcttc
      301 accaccatca ccggcgcgat gaagaacgcg ttcggcggat tgctgcaccg caagcggcac
      361 tggacacacg ctgtcattca cgacacgctg gtcgatctat tgcagatcca gcaggacatc
      421 cacgagggct tgttcgcggt gatggatggc acgttcacgg gcgacggtcc cggcccgcgc
      481 gccatgcgct ggcacgagaa agacatcctc ctcgcctcgg ccgatcaagt cgccatcgac
      541 gcgatctcgg ccaaattgca aggcttcgac ccgatgtcga tccgtttcat ccggcgtgcg
      601 cacgaagacg gcctgggcat cggcgatccg aagcaaatca aggtcgtcgg ctacgacatc
      661 gagcaagagc cggcgtgggg tttcgtgcag gaagacacct tcgccagccg gggtcagaag
      721 ctgatttacc acggtgcgct caagccgctc gaggggctgc tcctacgctc gccgatcgtc
      781 ccatggtcgt acttcgccag caacttctat cacaacgtgt actggtaccc gttcgtcggc
      841 cggccacgcg tgcgcgccgc gctgaacacg aaatggggca ggctgttcgc gcggtacggc
      901 gacggtaaag tggtcatgcc cggcatgcag ccggagacag tcgcgatagc ggcggcgggt
      961 gcggccctcg ccggcgggat gatcgcggcc gggtggatgc tgcggcgacg cagataggac
     1021 gaacgaccga cgacgaagga caaacgatcg atcaatcgcc cttcgtcatt accccaccgc
     1081 catccagata cgcttcggtt cgtcgccgcc cggccgccat gaggtggtcg gcgtgccgga
     1141 agtcccagaa cgcgatcggg tgctcgctcg agagccggat gtggtgaacc gtcaccccat
     1201 acagcgactc gtacacctgg atctcgcgcg ctccctggcc ctggatcagc aaatcgatcg
     1261 cacgcaagct gatggcgagc gtgccgcgca gcccgtcggt tcgcgtcgag gcagggctta
     1321 cattcagagc gtagatctcg gtcgcgccgc gctcgaccgc catctgaacc ggaaggaagg
     1381 acacgacacc gccgtccacg taccgcgcgc cgtcacactc ccacggcggg tagaaaccgg
     1441 ggatcgccgt cgtcgccatc aggccgtcga tgatgcgatc ggacggatcg tcgccgaagc
     1501 aacgcacctc tcccgtgtcg aggcgaaccg ccagggcata ggtgggcagt ctgagatggc
     1561 cgaagcacac tacgccgggc gggagtgttc gctccagata gcgctgcagc ggtgcgttag
     1621 gcgtcagacc gtcgcgcctg gtcaccagtc gccagatcat cgccagattg ccccggcgcg
     1681 acaccgcctc gtgggccgcc ctgcgccaat gctcggccag gcgatgtaaa ccctcgagcg
     1741 tcggatccat cgcaaggaat gtcgcattca tcgcgccggc cgatgctccc accaagagat
     1801 cgggccggat gcctctttcg agcaggacct gaagagcgcc gacctgaagc gatccgtaat
     1861 tgccgcctcc gctgagcaca aatgcaaccg tcatagcttt cgtttctcca agggccgtat
     1921 cggcgccggg taactactca ggcaaaccct tgcgaaggtt tccaatgcgc ctgtcggcat
     1981 gcgccgccag acgggcgccc gcgcctctga cggggaactc acgttaaacc ttcgcaaggg
     2041 tgagcagtta cggcgcttgt gtgttatccg cgcacggtgt acattatagc agccctctga
     2101 gaggcgccta tgggcatccg gaacatcggc cgcaagaccg tcggcgaggt gcgcgccgaa
     2161 atcaagcgcg gcgcgcgctt cgtcgtgtac gagtacgtga tctcgctgct ggtcgtcacg
     2221 cggcggcggg caagtcccgt gtactatatc gagccgggcc ggtcgccggc gcactttcac
     2281 tggccctatt cgctgctgac gctggcgctg ggctggtggg gatttccgtg ggggccggcg
     2341 ttcactatcg gagcggtgcg tgacaacctg cgcggcggcc acgacgtgac ggctaaaatg
     2401 ctcgagacga tcctggccgc agccgacaag aaaaggaagt gatgaacgtc atttgcgttg
     2461 cgacgaggaa gggcctcttc gtcttccagc gcgacggcgg cgactggcgc gaagtcgccg
     2521 ccggcctggg tggacacaac gtcaccagcg tgatcgcccg tgaaggcgtg atcctcgccg
     2581 ggacgcgaga gggtgttttc cgctcggacg acatgggccg gacgtggcgc gagatcaata
     2641 acggtctgag tgtgcgatac gtacgctgga tggcctacca cccagacgtg tcggatttgg
     2701 agttcgccgg gacggagccg gccggaatct ttgtgtcacg tgacggcggt gattcgtggc
     2761 gcgagtgccc tgaagtcgcg caggtgcgtg acgcacacgg ctggtcgctg ccgtactcgc
     2821 cgcgggccgg gtgtgtgcgc ggcctggctt tccacggcgc acgcgcctac gccgcgtgcg
     2881 aggtgggcgg cgtgcttcgc tcggacgacg gcggcgagac gtggcgtctg gcggcaggca
     2941 gctccggcga tcccgagcct tactatcccc ctgccccacg ggtgtgtcct gacgtacact
     3001 cgatcgccat ccatccctcg tcgccggatc tggtcttcgc gccgaccggc ggcgggctgt
     3061 atcgatcgag tgatggcggc aagacgtgga cgtccctgta tgactgctac tgccgggatg
     3121 tatggctcga tccgcgcgat cccgaccaca tcgtcttcgg cccggcggac ttcgtcgact
     3181 cgaatggccg catcgaagaa tctcgcgacg gcggccggac ctggacgtct gccgggacgg
     3241 gtctcaagac gccgtggcgg cgtcatttgg tcgagcgctt cgcgcagatc ggggacgagc
     3301 tgctcgccgt gctctcgaac ggggagctga tcgccgcgct gctggcgacg ctcggctggc
     3361 ggcgcatcct gccggacgtc acaggcgtgg aatgcgtgac ggccatgcgg taacgatagt
     3421 atgtaatccg tggttaatct tttggttgcg gctgagagtc gcgctatggt ataatcggat
     3481 cgagaatcaa catcaacggc cccgctttcc ggggtcgata cgtcctaaga acctatccga
     3541 caactggagt tcaggcttca gcctgcggca tctgaagcgg actagcccta aagggggcta
     3601 cgcacagtcc acactccggt agttttgata gactctaagc ggaacagcga atcgacgcgt
     3661 ttcctgttcg gcgtattgca ggagaggtgc catgtcaggt cacagccatt gggcaactat
     3721 caagcgcaag aagggcgcga acgacgccag gcgcggccag acgttcagca agcttggccg
     3781 tgagatcgcc atcgccgcgc gcgacggccc cgatcccgac aataacgtca agctgcgcct
     3841 gatcgtcaac aaggcgaagg cggcgggcat gcccaaagac atgatcgagc gcacgattcg
     3901 gcgcggcgca ggtctcgaca aagacgccgc tgcattcgaa gaactgatgt acgaaggata
     3961 cggcccgcac ggcatcgcca tgatggtcaa ggtcactacg gataatcgga atcggacggt
     4021 ctctgatata cgccgcatct tcacgcgcag tggcgggagc ctggccgagt ccggcgcggt
     4081 cgcctggaat ttcgagcaaa agggatacat ttcgatcccg atcgccaacc ccgacccgga
     4141 tcgcctgttc gacatcgccg tagaggccgg cgcggacgac gtggaaatca ccgaggatca
     4201 cctggaaatc tacaccgcgc cgaacgagct gcacgcggtc aacagcgcgc tcgaaaaggc
     4261 cggcctgaaa gccgagaccg tcgagctgac gatgaaaccc aggcagctca cgccgctcag
     4321 catgagcgac gcgcagagcg tgctgcaggt tgtcgatctg ctcgaagagc ttgacgacgt
     4381 gcagcaagtg tacgccagcc tcgatctacc ggaagaactg gtcaccgggt acgagccgca
     4441 ggcagcgtga ctggaagtta gaggttagaa gttggaggtt ggaagttgga ggctggccag
     4501 ccgacgatct aacctccaat ctccagcctc caatctctaa gacatgatct tccttggcat
     4561 cgacccgggc acggccatta ccggctatgg ctttatccgc gaagagggaa gctcgctccg
     4621 agccgtagcc tacggcgtga tcaccacacc gcccgacctg gcgctgccgc agcggctcgt
     4681 tctgctgcat cgcgcgctcg gcgatctcat ccggctccac cggcccgcgt ctgcggcggt
     4741 ggagcagatt tttttcagcc gcaatgcgcg cacggcgctg gccgtgggac atgcgcgcgg
     4801 cgtcgtgctg ctggccctgg cacaggccgg gcttccggtg gccgagtaca cgccgctcga
     4861 ggtgaaacag gccatcacgg gctacggccg cgcagacaag aaacagattc aagagatggt
     4921 gcgagtcctg ataggattgc cggaggtccc caggccggac gacgcggccg acgcgctggc
     4981 catcgccatc tgccatttgc actcgtcgcg gattcgggac ctggcgggag gcgcgccatg
     5041 atcgcgcggc tgcgcggcac agtggagtcg atcgataagg acggtctgat cgtcagcgtg
     5101 ggcgacgttg gtttccacgt gcgcgcctcg gctgcggcgc gcgagctggc cgggcggtcg
     5161 cgcagcgtcg atctgttcac acatatgcac gtgcgcgaga acgagatcgc gctttacggc
     5221 ttcgccagcg aagaggagct cgcgctgttc gagctgctgc tcggcgtcag cggcatcggc
     5281 cccagggtgg cgctgagcat cctggccggg gcgccgcccg agcggctgcg gcaggctatc
     5341 gccgccgaag aaatcgacgt gctcacgcgc gtgcccggcg tcggcgcaaa gacggcgaag
     5401 gcggtgatcc tccatctcaa ggacaagatc ggcgcggtac ccggtgtgcc ctggcccgcc
     5461 gcattcgccg acgacgacgt cgagatcgtc ggcgcattaa cggcgctggg ctacagcatc
     5521 gccgaggcgc aggcggcgat ggcgagcgtc ccgcgaggca gcggtgcgag cgtcgaggag
     5581 cgattgcgtc tcgcgctggc ctacttcgcg cgtccctgac gcctgccgct cgggccggca
     5641 ttagaaccca tccgataact gaaatgcagg tttcggcctg ccgcatctga ggcggactag
     5701 agtctgccgg attttggcga cagccatatc tggcggtcaa gcaggctaaa gcctccaacc
     5761 gccagatatg gcagcctgac cccactccgc attccttcgg ctgccccttc gactgcccct
     5821 tcggctacgc tcagggcaag gctcaggaca ggcgctgcgc gggcaagtga ggctgcactc
     5881 cagcgatttg ctaaactcca gtaaagtccg cgcttcggtg gttttgggat aggctctgag
     5941 agggcgtgtc aaagtgctgc agcaactctg acctgaatca taccaactcg atttcgtttc
     6001 agttacaaat cacaagtggg aacgccggac gcgttgaccc gccattccag atatgctatg
     6061 attttggaca aatcgaggta gctgcccctt tcttcggcat catccgacga cctcagcagg
     6121 ggcccgaatg aaccaacttg ccaaccccca gactgccacc acggctcgcc gtcccctgaa
     6181 cctgggcata cgcggcaaag ttaccattcc ctaccttgtc cttacgtttg cagtcgcgtt
     6241 cgtcggcgtc ttcatcgtga cgcgctacgt caccgactcg ttcgcggagc gcttcgccaa
     6301 ccagttagcc gacgcagccc gggtcgccgg cgaaggtttc gtgggtatcg agcagaggca
     6361 tctcgacatg tggcgcattc tggctgcgac ggagggagtg caggatgccg tcgccgcccg
     6421 cgatttgccc acgatacgcg cactcgttga cggcccggcg cggagtcgat ccgtagacag
     6481 tgtatggatc gtcgacctcc agcctatcgt cctggtcgga cttgaccgca cgccgaacgg
     6541 agactatacc gaagccaaca actttccatt cggagaattc gagccgctcg agcgcgcact
     6601 ccggggcgaa tctgacgaat tcggcgataa gttcgcgggg attcagttcc ggtccggcca
     6661 gccatacctc gtcacggcag gtcccctgga ggatggcgga aagttgttcg gcgcgatcct
     6721 gatcggcacg cggctcgatc gtgccctgcg ctcgatcgac cagcaggcgg ctgcgcgcat
     6781 cagcgcgtat gacacgaacg gcgatctcct tggcacgaca ctgctgggtg agggaggccc
     6841 tcctgaccag aatcgtctcc ctccggatca aatcaacgag atctttgcgc gagcggctgc
     6901 tcccgagtac ctgagcggcg acttgcagca cggctccacg ccgtatcgat tgctctacgc
     6961 tcccctccag attcgccaga gcgtgatcgg cgcctactcc gtcggtcttc cgagcgattt
     7021 cattttcctc aagagcgcgg acagccggct gctcttcgcc ttcatcttca cagcgatggc
     7081 gctgggggtc gtcgtcgtcg gtttcctgct gtcacgccgc ctcatcgcgc ccgtcctgca
     7141 actggtgcgc acctcgcagg ccgtcgcgag cggcgacctg aatcagcgca cggggctggc
     7201 cggcggcgac gagatcggcc tgctggcaaa cacattcgac gacatgacct acaagctgca
     7261 ggagcgcacg cgcgatctgg agctgctgct gcgggcgcat cgcgaagaag cgttcaagat
     7321 ccgcgcgatc ctgtccagca tcgccgatgg cgtgctggtg ctcgatccgc agggccgcat
     7381 catcatgatg aacgccgcag ccgagcacat cctgggcgac ttgtctgccg atttcatgac
     7441 gggcacgttc cgcgagcagc cgatgaatcc gatcgagccg ccgcccgaga gcatggcgct
     7501 catcgacagc gccgaggtgc gccgcttcga gctgaacaac cgcaccatca gcacgcacgc
     7561 cgcgcccgtc atcatccccg aaggtcatca actcggcacg gtcatcgtcc tgcgcgacgt
     7621 cacgcgcgag gccgagatag accgcttgaa ggatcacttc atcgagcaga tgtcgcacga
     7681 gctgcgcact ccgctcacgg ccgtcaaagg ctacagcgac ctgatactgc agaccggcgg
     7741 cagcgagata cctgagaagt acctggagtt cctgaacatc atcaaccggc acgccgattc
     7801 gctggtggcg atgatcaccg agcttctcga catctcgcag atggaggccg gggcgatgag
     7861 cctgcgccgg gagcgcatcg acctgaacga gctggtcgac gtggcgctca gcgaatggcg
     7921 gaggcggttc gcggagaagg agctcgcgct ggaggtgaac ctcgcgcccg agccggtggc
     7981 gatcacaggc gatcgccgcc gcctgaactg ggccatcagg caactggtca gcaacgccta
     8041 ccactatacg gagccgggcg gccgggccac ggtcagcgta gcgggcgatg ccgaggcggt
     8101 atccatcagt gtccaggaca ccggcacggg catcacgccg gaagatcaga aattcttgtt
     8161 cacgcggttc ttccgctcga ccacacgcgt ccattccaac gagcgtggtg tgggactggg
     8221 gctgtacatc gtgaaggcgg tcgccgaagc gcatcatggg aaagtcgaag tgcacagcgt
     8281 cgtcggtaaa ggcagcacct tccgcctgct cctgcctatc gaagaggtcg acgacgagct
     8341 gccggtcgtt aaaatgcctc aagacgcgcg aacatgagcc gccagccgcg ccgtgagccg
     8401 atcgcggcca ggctgccgta cgtgttgatg atcctggccg gcctggcctg catcgtcctg
     8461 attgcgctgt cctcggttgg gcagcagggc atcggctggc tggtcgtccc tgccggcctt
     8521 cagcccaact cgacagccga ctatagtctt caagtcggcg ccacactgcc ggcagtcgac
     8581 gccgaacagg ttgccatgtt gctggcgaca gacgaagccg ggctgcgcgt cacgcccaag
     8641 ccggggacga tcatcaccgt gccggcgggg cagttcacgc cggggccgac ggacgtcgcg
     8701 caggtgacgg ccacgccgag ggcaacgcgc acgcccacgc gcacaccgcc ctcaacccgc
     8761 acgccgactg ctacgccatc gcgcacgcga acctttacgc cgacctcgac tcgcgtgcga
     8821 acggcaacgc cgaccttcat cccaagcccg acgcctgtga tcccgacggc gacgaatacg
     8881 tctgcgccgc cgccaccgcc gccgccaccg ccgccaccgc cgaatacggc gacgtggacg
     8941 ccgacttcgc cgccgagtgt gacgccgtcg ccgacgaata cgccgagttc tacgaacgcg
     9001 gcgacgttga cctatacgcc aagcccgacg tcggttacgc ccaccaacac gccgtccatc
     9061 acgccgaccg tgaacccgcc tccgcctgcg ccgtcaagtc tcgcggtcgt gaatacaagc
     9121 tcctccctga ttagtctggt ctggtcgaac gagagcgcgt tggacgccga tttcgtggga
     9181 tacaacgtgt acatcagcac ggttccgggc gggccataca gcttcgtcgc cagcgccggc
     9241 acggccacga gttacgtcgt gagcgggttg agcagcggca caacctacta tttcgtcgtg
     9301 cgcggcgcgg acacagtcca ggagtcaggc aacagccccg aggcgagcgg cacgaccggc
     9361 gcggtcagca atccgtatcc cactcccccg ccctcctgcc caccgcctgc gccgcccgac
     9421 tgcggaaatg cgggaggccc gcccgacggc aactggtcgt acgtcgcgcc ggggcaggag
     9481 ctcatcctcg acctcggggc caataacggg attcttgacg gacctggctg ggacttcgtg
     9541 tactacgaac gtgagacggg cactacaggc tctatcggaa tggactgggt aaccatcgaa
     9601 ctctctagcg atcaggtaac ttggtatgtc gctttcacgt ggaatgccgg gagcaacgcg
     9661 caaaacagca atatcgctcc tttcgcctca cccacccctg atcccaatta ttgcgacctt
     9721 tgggcaggag ctgcttccga cgaacctatt ccgatggggg cttgcggcat aagctgggga
     9781 ggtctgtggg gcactccgcc aactccacag actggcatcg caattgacat cggacctgtg
     9841 atcccgacca ttccaccgga gggcttccgt tacatcagaa tacaagggat cgatccggct
     9901 caacctgccg agatcgacgg tatcgagcgc ctgaactgaa caggcgacag agagagactt
     9961 cggaagtctt caagggtagc ctggcttgcg gggtggcttg ataacgagac cccttcgcct
    10021 gcaaggtcgt accctgagac ttccgaagtc tatgcagtgc tggtagcgcg gagcgtcgcg
    10081 catgcttcga caggctcagc accggcctga tcgagcagtt tcttgggccg aacttctata
    10141 ccgtgcctcc tcgccatctt gtacctcagt cggctatatg ctattatcag gtcagtcgga
    10201 taaccctccg ataaagggta ttgcaattag gcaagtcaaa acaggaggca gatatggcac
    10261 aagtatctct cgatgaagcg cgagtcaaag agctgttcaa gcaggccatg ctggaacttt
    10321 tccaagaaca gaggggtttg ctttatgacc tgttcgtgga agtccttgag gatttggcac
    10381 tcgtaaacgc catacaagaa ggcgagtcta gcgagacggt cagccgagca gaagttttcc
    10441 aaatcttgga gagcgcggct tgaaagtcga gttcaaaagc agtttcgcta aagacttgaa
    10501 gaaagtcaaa gacaaagact tgaaggcacg agtcaaacag gttatcgaac ttgtcgaaaa
    10561 agccaagagc cttcaagaaa tacagggcac caagaaactc aagggtggtg accgttacta
    10621 ccgtatcagg attgaggatt acagagtcgg cctgactttg gagggtgaca ccgttacctt
    10681 tgtccgcttc ttgcatcgaa aagacattta cagatacttc ccgtgacaag gtagcaagct
    10741 cttcaaagct gtcaaccatc gtctcaagtg gtccggtatc catcggcaaa ctgatccgct
    10801 gacaaatggg acttggggct caaaaagctg cttcgctgcg ctccgcatga caatgatggc
    10861 ggcgcagccg gattatggtg tcggcgtcaa cagcgcggga tcgatcgtgg gcgtaatggt
    10921 cggagacggg gtaggcgtca cttcgactgg cgcgggcggc ttgcggcacg gatcgtagcc
    10981 cgcgccgacg atcaggcggt acgtgaccgg gctgttcgga tccggctgag agacgatatt
    11041 tccggcaggc acctggaaca gccgcgccaa ctggctcacg cgcgagcctt tgctcgtcgt
    11101 cgtatagtcg acgatcacgg tgtccgcacg aaggtcgacg tcagagtggg gcggcccaat
    11161 tacctcgaag ttgtagcgca gcagccgatc gatcgccaga tcgccccagt ccgggaagcc
    11221 cgacgcattc cacacctcga ccgcggcgct cgcctgtgac gccacgttgg cgggcggcgg
    11281 gtcgaacgcc tcggccagca ccagcggcag cacctctgaa ttgatgtgca aaacctgcgc
    11341 gccgttctcg gcgacgaaac cctgcgtcac gacggcgtcg atctggcgca gcttgatcgc
    11401 cgaaggatcc agctcgccgg cgatgccggc cagccacagg acgtcgttca gcgtgaggtc
    11461 ggtctggatc gacgccgata agtgattcca caactccggc actttgccga gcgtgccgtt
    11521 ctcgcgcgcc gcctgcagga gcgcaagccc caggttcaat atccggcgcg atcgatcaaa
    11581 gtcgttggtc gacaggcggg aacgggcaaa ccacaatgca tgcttgctgt cgagatagtt
    11641 atatccgggg acgatctcca gcgtgccggt gatcgtcgtg ctgagcgcat cgccggagag
    11701 gatgtcggtt tgcccttccg gcacgtctgg aaagatgtcg gtcagagggc agtcggccag
    11761 cacgcggacc gtcggcttat cgtcgccttc gcgtacaacg cccagcgtgt cgataatcgc
    11821 gcggaaggca ttgaagtcga tgcgcacgaa gtactgcacc tcgatgccga ggttgtactg
    11881 gatcgtctgc ttcaccaggc cggcgccgcc gccgggaaat ccgcttcgct cgccgtgcgc
    11941 gtcgacgacg ttgatgcggt tgtgctgcca gttgggcaca tacacccaca aatcgcgcgg
    12001 gatggacagc agcgtcacga acgtcggatt ctggcgcacg ctgatcagga tgatcgtgtc
    12061 gtcgcgccac gagctgtcgc ccggccggcg gtcactgccc aggacgacga cgttgatcgt
    12121 gtcgcggggc agggcgacga tgggcgccgg cgtcggcacg agcgtcgtgg cgtcggtgat
    12181 gacctcgggt gtgggcagcg gggtcaccgt cccggcgacg ccgggcagcg gcggccgcgg
    12241 cgtaggcgtg ccggtcggcg tggggcttgg cgtgagcgtc tcggtcggcg tcggcgtgtt
    12301 ggacggcgtc aaggtctccg tcggcgtgaa gctgggcgtc ggggaataac gcgtcgtcgg
    12361 cgtagctgtc gacgtggcga ccagcatcgc cacctccttt ccggcgctgc cggccaggaa
    12421 gatacctatc agcacgatca gcccgacgac gacaaacaca agcagccact cgacggcggc
    12481 tgctgagggc gaattcgaac gttcggattc catcaggccc tgaacaacag aagaaatcgc
    12541 tgtgtctcag ctcccaaaac cgcgcgatta taccatgcgc aaaccgaatt tacgagcgcc
    12601 ggaatcgagc ctctgcgaag cgccctttag agttaggcgg atccggccga agcctcgact
    12661 ccgatcggaa tgttcgactg cgagcaatac ctcatgcgcg aatagccggt caagttatgg
    12721 cgccgccggg acggacgccg gcatgggtgt cgcagtggca atgggtactg gcgcgcggcg
    12781 caggcacgga tcgaagttct ggccgagaat gacacgataa ggaaccgggc tgctcgcctc
    12841 tggctggtat accacgtcgg cgctggagac cctgaacaag ttaatcagtt gcgccaggcg
    12901 cgaccccttc tgcgtcgtcg tgaaatcgac gatggtcgtg cgctcgtaaa gccgatcggc
    12961 tataccgttg ggccacgcct ggaacccagc ccacgacagg cgctcggcgg cgacgcgatc
    13021 ccagtccggg ttgccgacgc cgttccacac ctcgacggcc gccggcgcct gcgaagccac
    13081 gttcggcaaa aactcggcga acgcttcatc gagaactggc tcaagctcgt cgtagttgaa
    13141 ggcgtacacg ctcgcgccgt tattcgacac gaagtaatgc agcattgtcc cgtcgatgaa
    13201 ccggctccga atgtgagtcg gatcgagctg cgtcccgagc tgcgccaggt acacgacgtc
    13261 gttgagcgtc aggtcggtct gcaccgcctg gctgagctgg tcccacagcg ccggcaactg
    13321 cccgaccagg ccctgctgct gaatggcgct ccacagcccg cgcaggacac gatgctggcg
    13381 gcgcgagcgg tcgaagtcgc tggtcgtctt gcgcgaccgg gcgtaccaca gggcatgttt
    13441 cccgtcgaga tggtaaatgc cggcagaggg tatgtcgatg gtgccggtca cgaccgtggc
    13501 aagctccggg ccggtgatga tgtccgtcac gccctccggc acgtcgggaa agatatcgta
    13561 cagcgggcag tcggcgataa cgtcgatgcc gccgacggcg tcgatcagcg ccttgaagcc
    13621 gccgaagtcg acgcgagcgt agtactgcac cggaatgccg aaattgtatt gaatcgtctg
    13681 tttgagcaat cccgggccac cacccggaaa gccgatccgc tcgccgcgcc catcggccag
    13741 attgatgcgc gtccactgcc agttgggcac gtacacccac agatcgcgcg gaatggacag
    13801 catcgtcagc gacggcacgt cgggatcgac gctgacaagt atcatcgtgt cggtgcgcca
    13861 cccaccctcg ttggggcggc ggtcactgcc catcaagatg atgttgagcg tgttcggctt
    13921 gaactcgcgc agcggcgcgg gcgagggagg cgtccaaaaa gtgcccgtta tcacttcaac
    13981 cggcggcagc ggcgtgacgg tcagtgcgag attgaccagg ggcggaggcg gcgaagtggg
    14041 cggcaacgtc cgggtcggcg tgggtgttgg cgtgcgcgtg cgagtcggcg tgggcgtgtt
    14101 ggtccaggtc gggctgggcg tcggcggcgt cgtcggcgaa ggggtcagcg tgatcgtcgg
    14161 cgtggcgatc gtcaagatca ccggattcgc gaaaggcagc agccgcatcg ccagcacgcc
    14221 gcagatgacg gtgagcaggc cgatgatgat cgcgatcagt atgaagtcga tagcggacag
    14281 cgacgaccgc cgggacgccg cgcgggtgcc catgtgagtc ctttcgcgcc gtgcaaaaat
    14341 ccctacagtg ccattatagg cgatgcgggg cagtcgtcaa atacgaggtc tgcacggata
    14401 gacggcgaca agcctacgga gtt
//
LOCUS       DBNHXB010000399         3176 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_282719, whole genome shotgun sequence.
ACCESSION   DBNHXB010000399 DBNHXB010000000
VERSION     DBNHXB010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_282719"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..527)
                     /locus_tag="AC162G_16080"
     CDS             complement(210..527)
                     /locus_tag="AC162G_16080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR5077375.1"
                     /translation="MLIVHVFAHVKPESVEDFKAATLENARSSVQEPGIARFDVIQQQ
                     EDPTRFVLVEVYRTPDDPARHKETAHYAKWRDAVADMMAEPRTSIKYENIFPNDDGWE
                     LVQ"
     gene            complement(552..>3176)
                     /locus_tag="AC162G_16085"
     CDS             complement(552..>3176)
                     /locus_tag="AC162G_16085"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5077376.1"
                     /translation="RAYGVFRHYLRHTFEIEEDDPPDVVRSKVARIARDRPPEQVARI
                     THAAEVLLALAAEPEEPLQSGEALKRNLFEAALAGWRDVAARAPSVVVLDDLHWADPA
                     SVELLQHLFQLSEEVPILFLCAMRPYRQSPGWQVKLMAERAYSHRYTEIELHPLSAVD
                     SESLVDNLLDNPDLPDHLRQLLLRKAEGNPFFVEEVARALIDSGAIVRDEPGTPWRAG
                     QHVDAAHIDIPDSVQALLMARIDRLDDDVRRTLQLASVIGRTFSYRVLERVVETPDAL
                     DQHLKMLQRADLIHLAAHTSEPVYEFRHELTRDAAYASILRRHRRAFHRRAGEAIEAL
                     FVEQLEEQADRLAYHFDEAADDERALKYTTLAGDVAARLYANAEAIAHYTHAVEIATR
                     GVASGEQLIYLYTSRGRAFEVSGRHKEATAAYRELERLARERSDRALELAALIPWATI
                     HSTYTAEFDPEQGQALSERALALARDMQDHRAEAKALWNLMLLTLFAGKGARQAVAYG
                     EQALAIARRHDMHEELAYILHDIARAYFEAGEVDPAWSAQEEALALWRELGDLPMLAD
                     TLTSAASRYYWVGELDKAMALAQEARRISESIGNSWGEAYSLSMISPIYLERGEADKG
                     IQALEDAIALGERANYWGAQVFDRAILAWVYGLLGDVERGFAAARLALAKTEEFGQPR
                     HPILAVLALLHLYGGNPEEAFATMHQAREGSGPDRPGAYPENTLIFDLIDGEVALAHH
                     DYEHVLQLAERAITAMRAKGVRSLRPNMLYLKGKALHSLGKTAEARQVLSEARAEIRA
                     LGLNHSLFLLPVLRALSEIEAQAGNALEAQALRKQAREIVEFIADHTGSPELRSSFLA
                     TPDARAVNNDQWSVISSQ"
BASE COUNT          525 a         1043 c         1015 g          593 t
ORIGIN      
        1 gggcgcttga cgaggttaag ccattccttt tgcacgacct tgacgtggtg gtcaatgggc
       61 ggaatctgag taccctgctc gatctcgatg aggtgatcct ccctgtggtg caagagcagg
      121 gcggtgcgtt cctcgcgttc gacgccgccg gccttgcggc catccgccgc tgagcgtccc
      181 agtgaaactt cacaccagac catcagccct cattgcacta attcccagcc atcgtcattc
      241 gggaatatat tctcatattt gatgctcgtc cgcggctccg ccatcatgtc cgccacggcg
      301 tcgcgccatt tggcatagtg cgccgtctct ttgtgacgag ccgggtcgtc cggtgtgcgg
      361 tagacttcga ccagcacaaa tcgggtgggg tcctcctgct gctggatgac gtcgaaacgg
      421 gcaatgccgg gttcttgcac gctgctgcgg gcattttcga gagtggccgc tttgaaatcc
      481 tccacagact cgggcttgac atgggcaaag acgtggacaa tgagcataag gcaccctttg
      541 ctgattactg ctcactgact actgattact gaccactggt cgttgttcac cgcgcgcgcg
      601 tcaggcgtgg cgaggaacga ggagcgcagc tccggcgacc cggtatgatc ggcgatgaac
      661 tcgacgatct cgcgcgcctg cttccgcagc gcctgcgcct cgagggcgtt gccggcctgc
      721 gcttcgatct cgctcagcgc acgcaatacg ggcagaagga acagactgtg attcaaaccc
      781 agcgccctga tctcggcccg ggcttcagag agaacctggc gagcctcggc agtcttcccc
      841 aggctatgca gggccttgcc cttcaggtag agcatgttgg ggcgcagcga gcgcacaccc
      901 ttggcgcgca tggccgtgat ggcccgctcc gctaactgaa gcacgtgctc gtagtcgtga
      961 tgggcaagcg ccacctcgcc gtcgatgaga tcgaagatca gcgtgttttc gggataagcg
     1021 ccgggccggt cagggccgga cccttccctg gcctgatgca ttgtcgcgaa agcctcttcc
     1081 gggtttccac cgtacaggtg cagcagcgcc aacacggcca ggatcgggtg gcggggctgg
     1141 ccgaactcct cggtcttggc gagcgcaagg cgggcggctg cgaagccgcg ctccacatcg
     1201 ccgaggaggc catagaccca agccaggatc gcgcggtcga acacctgcgc gccccagtag
     1261 ttggcccgtt ctcccaaggc gatcgcgtct tccagcgcct ggatgccttt gtccgcctcg
     1321 ccgcgttcca ggtagatggg gctgatcatg ctcaggctgt aagcctcgcc ccacgagttg
     1381 ccgatcgact cgctgatgcg ccgtgcttct tgggccagcg ccatcgcttt gtcaagctcg
     1441 ccgacccagt agtagcggct tgcggctgac gtcagggtat cggccagcat gggcaggtcg
     1501 ccaagctcgc gccagagagc gagggcctcc tcctgtgcag accacgccgg atcgacttct
     1561 cccgcttcga agtaggcgcg cgcgatgtcg tgcagaatgt aagccagctc ttcatgcatg
     1621 tcgtgccggc gcgcgatggc gagcgcctgc tcgccgtagg ccaccgcctg gcgggcgccc
     1681 ttgcccgcga agagtgtgag cagcatcagg ttccacagcg ccttggcctc ggcgcggtga
     1741 tcctgcatgt cgcgcgccag cgcaagggcg cgctcggaca acgcctgccc ctgctccgga
     1801 tcgaactccg ccgtgtacgt cgaatggatc gtcgcccagg gaatcaatgc cgcaagctcc
     1861 agcgcgcgat cgctgcgctc gcgtgccagc ctttccagct ccctgtaagc ggccgtagcc
     1921 tccttgtgcc ggccgctgac ttcaaaggca cggccgcggc tcgtatacag ataaataagc
     1981 tgctcgccgg aagccactcc gcgtgtggcg atctccaccg cgtgtgtgta gtgtgcaata
     2041 gcctccgcgt tcgcgtacag gcgggccgcc acgtcgccgg cgagcgtggt gtacttcagc
     2101 gcgcgctcgt cgtcggcagc ttcgtcgaaa tgatacgcga gacgatcggc ctgttcctcc
     2161 aactgttcga caaacaatgc ttcgatggct tcgccggccc ggcgatggaa cgcccggcga
     2221 tggcggcgca ggatcgacgc gtacgccgcg tcgcgcgtca gctcgtgccg gaattcgtac
     2281 accggctcgc tcgtgtgtgc cgccaggtga atcagatcgg cgcgctgcag catcttcaaa
     2341 tgctggtcga gcgcatcggg cgtctccacg acgcgctcga gcacccggta ggagaacgtg
     2401 cgcccgatga ccgacgccag ttggagcgtg cgccgcacgt cgtcgtccag ccggtcgatg
     2461 cgcgccatca gcagcgcctg cacgctgtcc gggatgtcga tgtgcgccgc atcgacgtgt
     2521 tgccccgccc gccaaggcgt gccaggctcg tcgcgcacga tcgccccgct gtcgatcagc
     2581 gcccgggcga cttcctcgac gaagaaaggg ttgccttccg ccttacggag gagcagttgg
     2641 cgcaggtgat ccggcaggtc ggggttgtcg agcaagttgt cgaccaatga ctcgctatcc
     2701 acggcgctga gggggtggag ctcgatctcc gtatagcggt gagaataggc ccgctccgcc
     2761 atcagtttca cctgccagcc aggcgactgg cggtaggggc gcatcgcgca cagaaagagg
     2821 atcggcactt cctcggacag ttggaagaga tgctgcagaa gctcgaccga cgccgggtca
     2881 gcccaatgga gatcgtcgag cacgacgacg gacggtgcgc gcgcggccac gtcgcgccac
     2941 ccggcgagcg ctgcctcgaa caggttccgc ttaagcgcct cgccgctctg cagcggctct
     3001 tccggttcgg ccgccagcgc cagcagcact tcggccgcgt gcgttattcg agcaacctgt
     3061 tccggtggcc ggtcccgcgc gatgcgcgcc accttgctgc gtaccacgtc cggcggatcg
     3121 tcttcttcga tctcgaaggt atggcgcaaa tagtgccgga acacgccgta ggcgcg
//
LOCUS       DBNHXB010000400         4712 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_618783, whole genome shotgun sequence.
ACCESSION   DBNHXB010000400 DBNHXB010000000
VERSION     DBNHXB010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_618783"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="AC162G_16090"
     CDS             <1..528
                     /locus_tag="AC162G_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077377.1"
                     /translation="DTQQRTLTLAALDAGRNADPTKPTRGEQDPPALVALIGRCDKID
                     TTNMTVDYVLVSNKGPTDQDPRGGGTVIAYPWVPGRWYDLTVVVPQNVSKDIDDYIRQ
                     WHTQRWIDANPGVPAATIRSAIGYYFPWWCGWGYWWWWWNGGDCIDIGVGEGESLDVE
                     QIVPMRVFMPTWPPK"
     gene            553..1566
                     /locus_tag="AC162G_16095"
     CDS             553..1566
                     /locus_tag="AC162G_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077378.1"
                     /translation="MSAARSMRWAGALAAGIALVLVSAQISPSAVTLPQAASPPDPQS
                     VTFAVGAGDPLVAQGVMPADILSAGGNVVILCDDLGLLCVDPITSDYDVIGGLSYGQD
                     FVSTEDLPIQFSVAQGSLGLPGTAVRVEAACSPGEPQADVFATAGNGSNVQALDGNGI
                     ACGANSSAGLYLEEGASSSDLSALDREACLLADPNCDGVPENPVFLTLAPGSPTLAAL
                     GASPADVLMAGVDYVPLEWADGVADLGLNASDAIDALCVLDNGDGVYGGGDQVLFSLA
                     PGSPTLVVRSFSPADLLQPGSPPSRRFTAASLGLQAGDDVDAMMCASVPFTVYLPVVL
                     KNQ"
     gene            complement(1736..2086)
                     /gene="rplT"
                     /locus_tag="AC162G_16100"
     CDS             complement(1736..2086)
                     /gene="rplT"
                     /locus_tag="AC162G_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR5077379.1"
                     /translation="MPRSKAGPNVRRRHNKIKQLTKGQRGARHRLWRKANEAMLHSLY
                     YAYRDRRQRKRQYRRLWIARINAAARQNGLSYSRLIAGLNKAGVTLDRKVLADMAVRD
                     ASAFARLAETAKNA"
     gene            complement(2252..2458)
                     /locus_tag="AC162G_16105"
     CDS             complement(2252..2458)
                     /locus_tag="AC162G_16105"
                     /inference="COORDINATES: protein motif:HMM:NF013771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large ribosomal subunit protein bL35"
                     /protein_id="HIR5077380.1"
                     /translation="MPKRKSHKATSKRFRLSGGGKLRRMRLGKHLRRRKAPRVLRSTD
                     KPETVTPKGLRRRVKRLTPYLDQK"
     gene            complement(2424..3026)
                     /gene="infC"
                     /locus_tag="AC162G_16110"
     CDS             complement(2424..3026)
                     /gene="infC"
                     /locus_tag="AC162G_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR5077381.1"
                     /translation="MAQIERGSTISSRELRINESIRAREVRVIAHDGQNLGVVPIQEA
                     LRLARERQLDLVEVSPQADPPVCRILDYGKYLYEQQKKEREAKKAQKIIEVKEIRLRP
                     KTDPHHRGFKVRDARKWLEQGMKVKVRIRFRGREITHPEVALQDLEEIAQELSDVAHV
                     EAQPNMEERTMLMVLAPQPKKKSSKEEPSRAKEEVSQSHV"
     gene            complement(3208..>4712)
                     /gene="thrS"
                     /locus_tag="AC162G_16115"
     CDS             complement(3208..>4712)
                     /gene="thrS"
                     /locus_tag="AC162G_16115"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR5077382.1"
                     /translation="DQPYKLELIDGLEKGGFDEYGNPLKEKPVISFYVDGPFVDLCRG
                     PHVESTGKVNPAAFKLLNITGAYWRGDEKNKQLQRVYGTAWETGDELEQYLWRLEEAK
                     KRDHRRLGKELDLYSVSDDVGPGLILWHPKGAMIRVVAEDFSRQAHLENGYEWVFSPH
                     IGRAHLWETSGHLDFYKEFMYAPMDIEGEAYYAKPMNCPFHIQIYKSHIRSYRDLPKR
                     YAEYGTVYRYERSGVLHGLTRVRGFTQDDAHIFCRPDQVESETSRALEFSLFILRSFG
                     LSDFTAYLANRPDKYVGDPADWDKATEALRRAVEAHAIPYKVDEGGGAFYGPKIDLKV
                     NDALGREWQLSTIQFDFNLPERFDLAYVGEDGQPHRPYMVHRALLGSMERFFGVLIEH
                     FAGAFPVWLSPVQAQIIPIADRHVVYAREVEQKLKAAGIRAEVDDSNERMQAKIRNAQ
                     LQKIPYMLIVGDKEQEAGAVAVRLRSGEDLKARPVDEFIALAREAIDGKK"
BASE COUNT          768 a         1488 c         1496 g          960 t
ORIGIN      
        1 gacacccaac aacgcacgct cacgctggcc gctttggatg ccggtcgaaa cgcagacccg
       61 accaagccaa cacgaggaga gcaggaccca ccggctctgg tggcgttgat cggccgctgc
      121 gacaagatcg acacgacgaa tatgactgtg gactacgtcc tggtcagcaa caaagggccg
      181 accgatcagg acccgagggg tggtggcaca gtcattgcgt atccatgggt gcccggtcgc
      241 tggtacgacc tgaccgtcgt tgtgccgcag aacgtgagca aagacatcga cgactacatc
      301 cggcagtggc acacgcagcg ctggatcgac gcgaatcccg gcgttccggc cgccacgata
      361 cgaagcgcca tcggctacta cttcccctgg tggtgcggct ggggctactg gtggtggtgg
      421 tggaacggcg gcgactgcat cgacatcggc gtgggcgagg gcgagagcct cgacgtcgag
      481 cagatcgtcc cgatgcgggt gttcatgccc acctggccgc cgaagtgatt gcgggaaggg
      541 atgaggtgat ccatgagcgc ggctcgatcg atgcgatggg ccggcgcgct ggcggcaggc
      601 atcgcgctgg ttttggtttc agcgcagatc tcgccctctg ccgtcacgct gcctcaggca
      661 gcgtcgccgc cggacccgca aagtgtgaca ttcgcagtag gcgccgggga tcccctcgtc
      721 gcccagggtg tcatgccagc ggacatcctg agcgccgggg ggaatgtggt catattgtgc
      781 gacgacctcg gcctgctgtg cgtcgatccg atcacgagcg actacgatgt gatcgggggc
      841 ctgtcttacg gccaggactt cgtctccacc gaggatttgc ccattcagtt ctcggtcgct
      901 caaggttccc tcggcctgcc gggcacggcg gtgcgggtcg aggcggcttg cagcccgggt
      961 gagccgcagg ccgacgtctt cgcgacggcg ggcaatggct cgaacgtcca ggccctggac
     1021 ggcaacggca tcgcctgcgg cgccaacagc agcgcagggc tatacctgga ggaaggcgcg
     1081 tcttccagcg acctgtcggc gctggatcgc gaggcgtgcc tcttggccga cccgaattgc
     1141 gatggcgttc ctgagaatcc ggtcttcctc acgctcgctc cgggatcgcc gacgctggct
     1201 gccctcggcg cgtctcctgc cgacgtcctg atggccggcg tcgactacgt gccgctcgag
     1261 tgggcggacg gcgtggccga tctgggactg aacgccagcg atgcgatcga tgcgctgtgc
     1321 gtcctcgata acggagatgg cgtgtacggc ggcggagacc aggtgctgtt ttccctcgcg
     1381 ccgggatcgc cgacgctggt ggtgcggtcg ttcagcccgg ccgacctgct tcagcccggc
     1441 tcaccgccgt cgaggcgctt cacagccgcg tcgctcggcc tacaggcggg ggacgacgtg
     1501 gacgcgatga tgtgcgcgtc cgtgccattc acggtttatt tgccggtcgt cctgaagaac
     1561 cagtgagggt aattggtaat tagtcagccg cgcacagcgt gtcattccga acacagcgtg
     1621 tcattccgaa cggagtgagg aatctctcgt ccggccggcg agtgcggccc gttgtggcag
     1681 agatgcctcg tcgtgccgcc tcggcatgac acgattacgg aacacgcttc acgttttacg
     1741 cgtttttggc cgtctcggcc agccgggcga acgccgatgc gtcgcgcacg gccatatcgg
     1801 ccagcacctt gcggtcgagt gtcaccccgg ctttgttcag gccggctatg agccggctgt
     1861 acgacaggcc gttctggcgc gccgccgcgt tgatgcgcgc gatccacagc cggcgatact
     1921 ggcgcttgcg ctgccgccga tcgcggtagg cgtaatacag cgagtggagc atggcctcgt
     1981 tcgcctttct ccacagccga tggcgcgcgc cgcgctggcc tttggtcagt tgcttgattt
     2041 tgttgtggcg ccggcgcaca ttggggccgg cttttgagcg tggcatttca ggtcaccttc
     2101 tttatcgaga cttccctcgg cccgcgggcc tgtggggagg tgacgcccgg atggggacgt
     2161 ctggttgtta agcccttgcg aaggttagac ccttcgctgc gctcagagct tgccctgagc
     2221 ctgcgaaggg gcacgccctt cgcaagggta gctacttctg atcgagatac ggcgtcaggc
     2281 gcttgacgcg gcggcgcagc cccttcggcg taaccgtttc gggcttgtcg gtagagcgca
     2341 gcacacgtgg cgccttgcgg cggcgcaggt gcttgcccag ccgcatgcgg cgcagcttgc
     2401 cgccgccgct caaccggaag cgtttagacg tggctttgtg agacttcctc tttggcacgg
     2461 ctcggttcct ctttgctgga cttcttcttt ggttgcggcg cgagcaccat cagcatcgtc
     2521 cgctcttcca tgttcggctg cgcttccacg tgagccacgt cgctcagctc ttgggcgatc
     2581 tcctccaggt cctgcagcgc gacttcggga tgcgtgatct cgcggccccg gaagcgaatg
     2641 cgcaccttga ccttcatgcc ctgctccagc cacttgcgcg cgtcgcgcac cttgaagccc
     2701 cggtggtggg gatcggtctt ggggcgcagc cgaatttcct tgacttcgat gattttttga
     2761 gctttcttcg cttcgcgctc tttcttctgc tgctcgtaca ggtacttccc gtagtcgagg
     2821 atccggcata ccgggggatc cgcctgaggg gaaacttcga cgagatcgag ctgtcgctca
     2881 cgggcgagtc gcagcgcctc ttgaatcggc acgacgccca ggttctggcc gtcgtgtgcg
     2941 atcacccgca cttcacgtgc gcgaatgctt tcgttgattc gtagctctct gctgctgata
     3001 gtgctccctc tctcaatttg tgcgatgctt gccggtcaac ggcgtgaaat gataacacac
     3061 tcaaattgat ccgtcaacca actcgaagac ctgacgggtc tccacccatg ttggggcgac
     3121 agttgcactg tcgcccggcg tccgcagtgc aactgcggac gaacggagat aatctaagtg
     3181 accggcaccg aggcgccggt cacttcgtca cttctttccg tcgatcgctt cgcgcgccag
     3241 ggcgatgaac tcgtcgaccg gcctggcctt gagatcctcg cccgatcgca ggcggacggc
     3301 gaccgcgccg gcttcctgct ccttgtcgcc gacgatgagc atgtagggga tcttctgcaa
     3361 ctgcgcgttg cgaatcttgg cctgcatgcg ctcgttcgag tcatccacct cggcccgaat
     3421 gccggcagcc ttcagcttct gctcgacctc tctcgcgtaa accacgtgcc gatccgcgat
     3481 cgggatgatc tgcgcctgca ccgggctgag ccagacgggg aacgcgccgg caaagtgctc
     3541 gatcaggacg ccgaagaagc gctccatgct ccccagcaag gcacgatgaa ccatgtacgg
     3601 acggtgcggc tgaccatcct cgccgacgta tgccagatcg aagcgctccg gcaggttgaa
     3661 atcgaactgg atcgtgctga gttgccactc gcggccgagc gcgtcgttca ctttcaggtc
     3721 gatcttcggg ccgtagaacg cgccaccgcc ctcgtccacc ttatagggaa tcgcgtgcgc
     3781 ttccacggcg cgtcgcagcg cttcggtagc cttgtcccaa tcggccggat cgcccacata
     3841 cttgtccggc cgattggcca aataggctgt gaagtcgctc aggccgaagg agcgcaggat
     3901 gaacaacgag aattcgagcg cgcggctggt ctcactctcg acctgatcgg ggcggcaaaa
     3961 gatgtgcgca tcgtcctgag tgaagccgcg cacgcgggtc aggccgtgca acacgccgct
     4021 gcgctcgtag cggtagaccg tgccatactc cgcgtagcgc ttgggcagat cgcgatacga
     4081 gcgtatatgg ctcttgtaga tctggatgtg gaatgggcaa ttcatcggct tggcatagta
     4141 ggcctcgcct tcgatatcca tcggcgcgta cataaactct ttgtagaagt cgagatggcc
     4201 ggatgtctcc cacaggtgag cgcggccgat atggggcgaa aagacccact cataaccgtt
     4261 ttccaggtga gcctggcggc tgaagtcttc ggccacgaca cggatcatgg cacccttggg
     4321 atgccacaag atcaggccag gccccacgtc gtcggagacg ctgtacaggt ctaactcctt
     4381 gcccaatcgc cggtggtcgc gcttcttcgc ttcctccagg cgccacagat actgctccag
     4441 ttcgtcgcct gtctcccacg ccgtgccgta tacgcgctgc aattgcttgt tcttctcgtc
     4501 accgcgccag tacgcaccag taatgttgag cagcttgaag gcagccgggt tgaccttgcc
     4561 cgtgctctcg acgtgcgggc ctcggcacag gtcgacgaac gggccatcga cgtagaacga
     4621 gatgaccggc ttctccttga gcgggttgcc gtattcgtcg aagccgcctt tttccagccc
     4681 gtcgatcaac tcgagcttgt acggctggtc ct
//
LOCUS       DBNHXB010000401         4812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4142555, whole genome shotgun sequence.
ACCESSION   DBNHXB010000401 DBNHXB010000000
VERSION     DBNHXB010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4142555"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="AC162G_16120"
     CDS             complement(<1..787)
                     /locus_tag="AC162G_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077383.1"
                     /translation="MSDEYVYRLSRDADTQEHGRASYRRLGLICGAAIGLGLALGAWA
                     PDFVARLDVPMQDNLPGIALGALALALLCALAGRLSARIDRAAIDVLIWLATGTVSYF
                     VIARAPYEVRTGLVGLLDSRFAGLPIYPYDGFGHGRAAFFVMLFFFLLGITHAIRCEA
                     LRGSLGPRDRPTTQTRFLLAFHLAAALGVGLIADGAVNAPLRIAPQLVNEAIRTGRTY
                     EGDLFELSRESSLNYNAISGVRDQMSERYTLQVGESDLVESLTV"
     gene            complement(784..1377)
                     /locus_tag="AC162G_16125"
     CDS             complement(784..1377)
                     /locus_tag="AC162G_16125"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5077384.1"
                     /translation="MSATEVATTSHFEAPNPTPTIDRLAPPPTVASPTQADEGAYLYW
                     LNCQPCHGDRGQGLTDEWRMQYPEEDRNCWNTGCHGETPYESGFKLPEKVPALIGEGS
                     LAAYDNFGQVHAFMRAAMPFQAPGSLKQEEYLAITAFLAREHGVWNAAQPLDEARLRN
                     VPVRPTPDDWTAVVAGAAGLLAAGVVGAWLWRRRSSP"
     gene            complement(1498..1863)
                     /locus_tag="AC162G_16130"
     CDS             complement(1498..1863)
                     /locus_tag="AC162G_16130"
                     /inference="COORDINATES: protein motif:HMM:NF037832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HIR5077385.1"
                     /translation="MNGRPIKLLLDEHIWEGLAEALTQRGYDVVHIIHTGQRSIDDKP
                     LLELAAAQGRALLTYNARHFVPLVRFWYEAGREHAGVILSIQLTPAQLLKQVERLLAT
                     LSADELKNTVRWLQEFKVD"
     gene            complement(1860..2282)
                     /locus_tag="AC162G_16135"
     CDS             complement(1860..2282)
                     /locus_tag="AC162G_16135"
                     /inference="COORDINATES: protein motif:HMM:NF016167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR5077386.1"
                     /translation="MSTVTLSETTYQWLRRRAQESAQTPDQVADELLRQQLAPKHAYV
                     EVVQKIAGPQAVIKGTRIPVSVIVGYLRIGETPESLTGRILPHLTLAQVYDALSYYHD
                     HQDEIEQELRENTEEYSRAYLREHLGEEGYMRVTGQAK"
     gene            complement(2298..2591)
                     /locus_tag="AC162G_16140"
     CDS             complement(2298..2591)
                     /locus_tag="AC162G_16140"
                     /inference="COORDINATES: protein motif:HMM:NF016167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR5077387.1"
                     /translation="MKQSAGANSKKRNAKPKLLGRYIVADPRICHGQPTFRGTRIMVW
                     QVLEMVASGMAWETIVEQCHHSIDEEAITEAVSLASKAFLEHAREHLVEPTAA"
     gene            complement(2732..3784)
                     /locus_tag="AC162G_16145"
     CDS             complement(2732..3784)
                     /locus_tag="AC162G_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077388.1"
                     /translation="MATGTVPAGLRPGSIDIEGLTLREPRRLEKLLGPELARVIYGLF
                     SNRLSIAGMIIVLGFIVVALAAPVLAPPLRGRDPTVIPRDGFGPEPKPPGTVWRSNPP
                     PVPGWYTTLTGQDQWVHILGTTAQQFDIWYGVIWGARTALFVGVVIVGITVIIGVIVG
                     SVASYFGGWIDDVLMRITEIFMAFPFLLAALTLASILVPAFGRGIWPAMIALIVFGWM
                     QYARLVRSDILSVKERDYVMAARVIGARDSRILGRHILPNAIFPTLVLASLDIGSIVL
                     AFAALSFLGVGTEVGYADWGQIVSFARNWILTLDKHWWIVIYPGLALILFGLGWNLIG
                     DALRDILDPKLRGARA"
     gene            complement(3877..>4812)
                     /locus_tag="AC162G_16150"
     CDS             complement(3877..>4812)
                     /locus_tag="AC162G_16150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077389.1"
                     /translation="PHTEGAINAVIRRYGLDDPFLVQYKNWLIGRVDGVTGETVGGVL
                     RGDLGWSRTGNSKVIELLGRRLPATIELALWAAGPIILIGIWLGVKAAVNHNKPIDQG
                     ARVFAIVGYSFPTFVFGLLVLLVFYARLEWFPPDRLSDWAIRVVNSSEFVRYTQLNTI
                     DGLLNGRFDILLDALRHMVLPIVTLSYLSWALLLKITRSSMLESLRQDYVTTARAKGL
                     RERDVINRHARRNALLPVATIGGLTVVALLNGVVITETIFDYPGIGSAVARAASSLDV
                     LTVLGFVLFNATLLIVANLIVDVMYALLDPRVRLG"
BASE COUNT          970 a         1570 c         1428 g          844 t
ORIGIN      
        1 ttaccgtcag ggattccacc agatcggact cgcccacctg cagcgtgtag cgctcgctca
       61 tctggtcgcg cacgcccgag atcgcgttgt agttgaggct gctttcgcga ctcagctcga
      121 acaagtcacc ttcgtaggtg cggccggtgc gaatcgcttc gttgaccagt tgcggcgcga
      181 ttcgcagcgg cgcgttcacg gctccgtcgg caatcaggcc gacgccgagc gcagccgcca
      241 ggtggaacgc caggagaaat cttgtctgag tggtcgggcg atctctcggg ccgagcgagc
      301 cgcgcaacgc ttcacatcgg atggcgtgcg tgatacccag cagaaagaag aaaagcatga
      361 cgaagaaagc cgcgcgcccg tgcccaaagc cgtcgtaggg atagatgggc aggcctgcga
      421 agcgactgtc cagcagcccg accaacccgg tgcgcacctc gtacggcgcg cgagcgatca
      481 cgaagtacga aacagtgccc gtcgccagcc agatgagcac gtcgatcgcc gcccgatcga
      541 tgcgtgcgct caggcgaccg gccagcgcgc acagcagcgc gagcgcgagc gcgccgagcg
      601 cgatacccgg tagattgtct tgcatcggca cgtcgagccg cgcgacgaaa tccggcgccc
      661 acgcgccgag cgccagccca agcccgatgg ctgctccgca aatcagcccc agccggcgat
      721 aactcgcgcg cccgtgttcc tgcgtgtccg cgtcgcgcga gagtcggtag acatattcgt
      781 cactcatggc gaacttctcc ggcgccacaa ccacgcgccg acgacaccgg ccgccagaag
      841 gccggctgcc cctgctacga ccgcggtcca atcgtccggc gtgggtcgca caggcacatt
      901 gcgtaaacgc gcctcgtcga gcggctgcgc cgcattccac acgccgtgct cccgcgcgag
      961 gaacgccgtg atcgcaaggt attcctcttg cttcaacgag ccaggcgcct ggaatggcat
     1021 ggccgcgcgc atgaacgcgt gcacctgccc gaaattatcg taggctgcca ggctgccttc
     1081 gccgatgagc gctggaacct tctccggcag cttgaaaccg ctctcgtagg gcgtctcgcc
     1141 gtggcagccc gtgttccaac agttgcgatc ctcctccggg tactgcatgc gccattcgtc
     1201 ggtgagcccc tggccgcgat cgccgtggca gggctggcag ttcagccagt aaagataggc
     1261 gccctcgtcg gcctgcgtgg gggatgcgac ggtcgggggc ggggcgagtc ggtcgatagt
     1321 cggggtgggg ttgggcgcct cgaaatggct cgtagtggcg acttcagtcg ctgacacaac
     1381 gactgaagtc gttactacca cctccgttac gtgtgaattc agtctgtcag gagaaatgcc
     1441 agcgacaaag agcaaaacca caacgagacc cagttgcaaa tgcaccctga gcatgtatca
     1501 gtcaactttg aattcctgca accagcgaac cgtgttcttc agctcgtcgg ccgagagtgt
     1561 ggccaacagc cgctctacct gcttcagcag ctgagccggc gtcaattgga tggagagaat
     1621 gactcccgcg tgttcgcggc ctgcttcata ccagaaccga accagcggta caaagtgacg
     1681 agcgttatat gtgagcaaag cacgtccctg ggccgccgcc agttccaata acggtttgtc
     1741 atctatacta cgctgacctg tgtgaatgat gtgaacgaca tcataaccgc gctgcgtgag
     1801 tgcttcggcc agaccctccc agatatgctc atctagcagg agtttgattg gcctgccgtt
     1861 catttggcct ggcctgtcac tcgcatgtaa ccttcctcgc ccagatgttc gcgcaaataa
     1921 gcgcggctgt actcttccgt gttctcgcgc agttcttgct caatttcgtc ctgatgatcg
     1981 tgatagtaac tcagtgcatc gtagacctgg gccagcgtca gatgtggcag aattctccct
     2041 gtcaacgact caggggtctc accgatacgc agatagccga cgatcacact cactggaatg
     2101 cgcgtgcctt taatcacagc ctgcgggccg gcgatcttct ggacaacttc aacataggcg
     2161 tgcttcggcg cgagctgctg gcgcagcaac tcatcggcga cctgatcggg cgtctgggca
     2221 ctctcttgcg ccctgcgtcg cagccattga taagtcgtct ccgaaagtgt taccgtactc
     2281 atagataacc tcaacgttca cgcggccgtt ggctccacaa gatgctcacg agcatgctcg
     2341 aggaacgcct tgctcgccag cgatactgct tcagtgatgg cttcttcgtc aatgctatgg
     2401 tgacactgct cgacgatcgt ctcccacgcc attccgctgg caaccatctc caagacctgc
     2461 cagaccataa tgcgcgtgcc gcgaaaggtg ggctggccgt ggcaaatcct cggatctgcc
     2521 acaatatagc gccccagcaa tttgggtttc gcgtttcttt tcttgctgtt cgcaccagcg
     2581 ctttgcttca ctgcgacaac tccttgtcta ttctcttgtc taattatacc ccacaacaaa
     2641 caggcgagcc gccgaaccgg tggcccgccc ctcatagact gaaacccaat cacgcttcac
     2701 gtttcacgct tcacgtttta cgcttcgctc atcacgctcg cgccccgcgc agcttcggat
     2761 cgagaatgtc gcgcagcgcg tcgccgatca ggttccagcc caggccgaac aggatcagcg
     2821 ccaggcccgg atagatcacg atccaccagt gcttgtcgag cgtcaggatc cagttacgcg
     2881 cgaagctgac gatttggccc cagtcggcgt atccaacttc ggtgcccacg cccaggaagc
     2941 tcagcgcggc gaacgccagc acgatgctgc cgatgtcgag cgaggccagc acgagcgtcg
     3001 ggaagatagc gttgggcaaa atatgccggc ctaaaatgcg cgaatccctc gcgccgatga
     3061 cgcgcgccgc catcacgtag tcacgttctt tcacgctcag gatatcgctg cgcaccagac
     3121 gggcgtactg catccagccg aagacgatga gcgcgatcat cgccggccag atgccgcgcc
     3181 cgaacgccgg caccaagatg gacgccagcg tcaaggcagc cagaaggaag ggaaaagcca
     3241 tgaaaatctc ggtgatgcgc atcagaacgt cgtcgatcca accaccgaaa tacgaggcga
     3301 ccgatccgac gatcacgccg atgatcaccg tgataccaac gatcacgacg ccgacgaaca
     3361 gcgccgtgcg cgcgccccag atgacgccgt accagatatc gaactgctgg gcggtggtgc
     3421 ccaggatgtg cacccattga tcctggcccg tcaacgtcgt gtaccagccc ggcaccggcg
     3481 gcggattgga tcgccagacc gtgcccggcg gtttaggctc gggaccaaac ccgtcgcgtg
     3541 ggatcaccgt cggatcccgg cccctcaacg gcggcgccag caccggcgcg gccagggcga
     3601 ccactatgaa acccaggacg atgatcatcc cggcgatcga caggcgattc gaaaataggc
     3661 cgtagatgac gcgggccagc tccggcccga gcagtttctc caggcggcgc ggctcgcgca
     3721 gcgtcaagcc ttcgatgtcg atcgagccgg ggcgcaatcc ggccggcact gtgcctgtgg
     3781 ccataactgt cctcctcaat atctgcaaaa ccagcagagg cggctcccat gccgccgggc
     3841 gtgggagccc ggcccgctaa cctaacagta ttggtcttaa cccaaccgca cgcgcggatc
     3901 gagcaacgcg tacatcacgt cgacgatcag gttggcgacg atcagcagcg tcgcgttgaa
     3961 cagcacgaag cccagcaccg tcagcacgtc caggctgctc gccgctctgg cgacggcaga
     4021 accgatgccg gggtagtcga agatcgtctc ggtaatcacc acgccgttga gcagcgcgac
     4081 gaccgtcaac ccgccgatcg tggcgaccgg gagcagcgcg ttgcgccggg cgtgacggtt
     4141 gatcacgtcg cgctcgcgta gccccttggc gcgcgccgtc gtcacgtagt cctggcgcag
     4201 cgattcgagc atcgacgagc gcgtgatctt gaggagcagc gcccaggaca ggtacgagag
     4261 ggtgacgatc ggcagcacca tgtggcgcag cgcgtcgaga aggatgtcga aacgtccgtt
     4321 gaggagcccg tcaatcgtgt tgagttgcgt gtagcgcacg aactcgctcg agttgacgac
     4381 acggatggcc cagtccgaca agcgatctgg cgggaaccac tccagccgcg catagaagac
     4441 cagcagcacc aacagcccga agacgaacgt agggaacgaa tagccgacga tagcgaacac
     4501 gcgcgcgccc tggtcgatcg gcttgttgtg gttgacggcg gccttcacgc ccagccagat
     4561 gccaataagg atgatcggcc cggcggccca cagcgccagc tcgatcgtcg ccggcaggcg
     4621 gcgcccgagc agctcgatga ccttgctgtt accggtgcgc gaccagccca ggtcgccgcg
     4681 cagcacaccg ccaacggtct cgcccgtgac cccgtcgacg cggccaatca gccagttctt
     4741 gtactgcacc aggaacggat cgtccagccc atagcgccgg atcaccgcat tgatggcgcc
     4801 ctcggtatga gg
//
LOCUS       DBNHXB010000402         2582 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2801776, whole genome shotgun sequence.
ACCESSION   DBNHXB010000402 DBNHXB010000000
VERSION     DBNHXB010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2801776"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="AC162G_16155"
     CDS             <1..1252
                     /locus_tag="AC162G_16155"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077390.1"
                     /translation="GGMRMSTRHLRTRLSVVIGITGLWLVAVLMFTTAQWGSADAHPL
                     QLTFASPILSLAKTVSQPAPKPNDLITYILAYSNTQAGAQAFNVRLYDFLPAGVQFQS
                     AAPPATSYDNGVLLFTAPSIGPATSNMTVSVTVRVLEGYAQLYNHALLTADGVTPTQA
                     SLLTGVQQPAAWLNLTKTGLRAVLVNGQLVYTLICENRSGITVNDVTVIDVLPAGLSF
                     AGASPSPSTQTPPVLSWSLGNLGPGEKRTIVMTTTAPASAGAIVNTALADARQRVMTQ
                     TLLATQVITEGAILDVAKSGPEVVNPGGRLVYTVRYRNVGNIPAAGVVLTDTFPADMS
                     VIAVDPPATSLTTQRGVWSIGTVGPASAKYTIVITATVRGSAGRTLLNVVDIRGQNGF
                     PGHAEWQTQVRPILVYMPIIRRDR"
     gene            1402..1797
                     /locus_tag="AC162G_16160"
     CDS             1402..1797
                     /locus_tag="AC162G_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR5077391.1"
                     /translation="MKTLTLKENQVIYAAAIGESLREGEAIILESDGRPIAAIVPIDE
                     YHAFRAWRVSRQQEVVPHDWPEDRTLEEVVADIQRRGPGVPNFHEATASLADLLANAP
                     HDPDFDLEEWEREWGKIDSEIEAKDEQNG"
     gene            1794..2201
                     /locus_tag="AC162G_16165"
     CDS             1794..2201
                     /locus_tag="AC162G_16165"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR5077392.1"
                     /translation="MTSYLLDTNHASPLVTRDHPLRRRIHDAIQTGDTFAMTVANLAE
                     VLYGIGTLPRAAQNRSEWERLRQAFRVYAIEEQDAVESAEIRVCLRRSGRQIGTIDAM
                     LAVVAIRYSLVLLTADRDFDAVPGLQQANWLAA"
     gene            complement(2286..>2582)
                     /locus_tag="AC162G_16170"
     CDS             complement(2286..>2582)
                     /locus_tag="AC162G_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077393.1"
                     /translation="YGYAFEAFDEAGNPIEQRFNQDVLIVFPYSEAELIAGGIQESHL
                     KPAYFSTTTDSWTFPSSYIVDEINNLVAMQIDHFTDFALTSSPQFEVFLPVVVR"
BASE COUNT          556 a          751 c          775 g          500 t
ORIGIN      
        1 aggaggcatg cgcatgagca cccggcacct tcgcaccagg ctttcagtcg tgatcggcat
       61 aacgggactg tggctcgtgg cggtcttgat gttcaccacg gcgcaatggg gcagcgccga
      121 cgcgcacccc cttcaactga cctttgccag tcccatcctc agcctcgcta agacggtcag
      181 tcagccggcg cccaagccga atgatctgat cacctacatt ctcgcttact cgaacacaca
      241 ggccggcgca caggcgttca acgtgcggct gtacgatttt ctgccggccg gcgtgcaatt
      301 ccaatcggcc gctccgccgg caacctccta tgataacggt gtgctgctct tcactgcgcc
      361 ttccatcggc ccggcgacgt ccaacatgac ggtcagcgtc accgtgcggg tcctggaagg
      421 gtacgcgcag ttgtataacc atgcgctatt gacagccgat ggcgtcacgc ccacgcaggc
      481 ctcgctattg acaggcgtgc agcagcctgc cgcctggctg aacctgacca agaccggcct
      541 gcgtgccgtg ctggtcaacg gccaactggt ttacacgctg atctgcgaga accggagcgg
      601 catcactgtg aacgacgtga ccgtgataga cgtgctgcct gccggcctat ccttcgcagg
      661 cgcctctccc tctccgagca cgcaaacacc gccggtcttg tcatggtcgc tgggcaactt
      721 ggggccaggg gagaagcgga cgatcgtgat gaccactaca gcgccggctt cggcgggcgc
      781 aattgtcaat acggcgttag cggatgcccg gcagcgggtg atgactcaga ctttgttggc
      841 aacacaagtt atcacggaag gggccattct ggacgtcgct aagagtggtc ctgaggttgt
      901 gaacccgggc ggcaggctgg tgtacaccgt ccgatatcgg aatgtaggga atattccggc
      961 cgcaggcgtc gtgctgaccg atacctttcc cgccgatatg agcgtgatcg ccgtagaccc
     1021 gccggccaca agtctgacga cccagcgcgg cgtgtggtca atcggtacag tcggtccagc
     1081 ctctgcaaaa tacacgatcg tgatcacagc cactgtgcga gggagcgccg ggcgaacgct
     1141 gctcaacgtg gtcgacatca ggggacagaa cggcttccca ggtcatgcag agtggcagac
     1201 gcaggtgcga cccatactcg tgtacatgcc gatcatcagg cgagacagat agaattgctc
     1261 gaagccgcac aggcgctgta ggaaaggatt ggccgcgcca cacgtcaggc gaaagccatc
     1321 taatcagaag attgctggct tgcccgggtc gggtgcgctg tgctatagtt accgacaaac
     1381 aaagcagaat agaggcgtat cgtgaagacg cttactttga aagagaatca agtcatctac
     1441 gcggcggcga tcggcgaatc gttgcgcgag ggcgaagcga tcattctgga aagtgatggt
     1501 cgcccgatcg cggcgattgt gccgattgac gaataccacg cctttcgcgc gtggcgagta
     1561 agccggcagc aagaagtggt tccgcacgac tggcccgaag accgaacgct cgaagaagtc
     1621 gtagccgata ttcaaaggcg agggccaggc gttccaaact ttcacgaggc aactgcttcc
     1681 ctggcagacc ttctagcaaa tgcgccccat gatcccgact ttgatctgga agaatgggaa
     1741 cgcgaatggg ggaagattga ttcagagatt gaagctaagg atgagcaaaa cggatgacga
     1801 gctacctgct ggatacgaat catgcctctc cactcgtgac acgggatcat ccgcttcggc
     1861 gtcgaattca tgatgcgatt cagacgggcg acacctttgc gatgacagtg gcaaatctag
     1921 ctgaagttct ttacggcatt ggcactctgc cacgtgccgc gcaaaatagg tcagaatggg
     1981 agcgattacg tcaggcattc cgtgtctatg cgattgaaga acaagacgcc gttgaatctg
     2041 cagaaattcg agtatgcctt cgccgcagtg ggcgacagat cggcacaatc gatgcgatgt
     2101 tggcagttgt cgcgattcga tacagcctag tactgctgac cgccgatcgc gattttgacg
     2161 cggtgcctgg ccttcagcaa gccaattggc tggcagcctg agtgcaccgc acccgctcca
     2221 accccccaag tctatctcca ggaagacgtg cgacgcaccc tgaaggtgtg tcgcacgtct
     2281 tgcccctagc gtaccacgac cggcaggaac acctcgaact gaggcgacga ggtgagcgcg
     2341 aagtcggtga agtggtcgat ctgcatcgcc accaggttgt tgatctcatc gacgatgtag
     2401 gaactcggga acgtccacga gtcggtcgtc gtcgagaagt acgccggctt caggtggctc
     2461 tcctggatgc cgcccgcgat tagctcggcc tcgctgtatg ggaagacgat cagcacgtcc
     2521 tggttgaagc gctgctcgat cgggttgccc gcttcgtcga acgcctcgaa cgcgtagccg
     2581 ta
//
LOCUS       DBNHXB010000403         7240 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2969370, whole genome shotgun sequence.
ACCESSION   DBNHXB010000403 DBNHXB010000000
VERSION     DBNHXB010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7240)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7240)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7240
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2969370"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2088
                     /locus_tag="AC162G_16175"
     CDS             <1..2088
                     /locus_tag="AC162G_16175"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5077394.1"
                     /translation="TILVVDDDRQIAEFLDDLLRQFGHRVVNAYGGQEALDVLASPEA
                     GGVDLILLDVLLPDMSGTEVCQRVKASPATAHIPIIMVTGLDRPADKTRGLELGADDY
                     VTKPFDPRELVARVGAMLRLRRAERELRARNRALAALNAVADTIGRAVELPDVLNTAL
                     DQVLATLDMEAGTITLTHWDGSQIPAARRWQALDLSSGVEAAEQVARSGAPALLTLSV
                     RGRDHAAACVPLRSRDRVVGTLLVAGKPGIDSAGLELLGAIGSQVGGAIERGRLFDAA
                     QRRSDDLAILNDITRAVTSSLDVDMVLARAMRSIREILRVEAGSLILADEDTGELRFR
                     KTLNREQEMIVDTTLRPGEGLIGQAVATLDPILLNDAPSDARFAPHLDRITGVVTRGA
                     LVAPLIVKGRAIGAIEVINKLDGPFTHDDLEMLQFLAASVAVAVENARLYGELAEFTR
                     QLERSQEQLIQAEKVAATGRLAASIAHEINNPLQAIHNCLHLVTHRKMEPEKEAYYLN
                     LAQEEVQRLIHIVQRTLDFYRPSQGRHTLTDVNNVIESVLALANKKVEHAKVHVHRRL
                     SPDLPKLNAVADQLTQVFLNLIVNAVEAMPGGGDLAITTLHDESHVRVRISDTGPGLN
                     AEEAKRIFEPFYTTKRSGTGLGLAVSYGIIQRHGGEIAVDSAPGRGATFIVSLPVNRA
                     GVAVENEPPAIRV"
     gene            2097..2891
                     /locus_tag="AC162G_16180"
     CDS             2097..2891
                     /locus_tag="AC162G_16180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5077395.1"
                     /translation="MDANSSFHILIVDDEAAMRATLSEILGGEGYRASAVESGEAALD
                     ILRVTPIDLMLLDLKMAGLDGLQVTETAQSISPDTVIVMLTAHGTLESAITAMRRGAH
                     DYLLKPASVPEILAAVERGLRHRAQALKQRDLITLMQRAIQELQVPGSGAGGEGAGPD
                     RFLQIRGVSLDLQRHVATANGKLLDLTLTEFKLLAYLMQHPDQVIAPRELVSAVQGYE
                     ADEHEARAIIRVHVRRLRQKMEPDPDNPEYVLNVRGVGYLFATTSP"
     gene            complement(2904..3737)
                     /locus_tag="AC162G_16185"
     CDS             complement(2904..3737)
                     /locus_tag="AC162G_16185"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR5077396.1"
                     /translation="MTTRKPAFIGTLLAGLALALSGCMGGNVTAEEIVAQSFDTSASP
                     RVVVETYNGHIEVTAGAEAKVLAEVTKHGAGFSQAEAKDNLKNVEVTMTQEGDTVRIV
                     ARRTGGPLFMGNSGASIELSVPAGVSLDLHTSNGHIRSNGVTGDIAMDTSNGALEVDG
                     GGGRLDLATSNGRIDVEARDAIVDARTSNGQIDFRGSLAEGSHDFRTSNGRIEITLPS
                     GAQFRIDASTSNGDVSTEFPVSRSGSSRDEELHGVVGENPDVSITATTSNGDIDIRKG
                     E"
     gene            4061..4888
                     /locus_tag="AC162G_16190"
     CDS             4061..4888
                     /locus_tag="AC162G_16190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HIR5077397.1"
                     /translation="MPGKRILIVDDETKVGFFLKETLESLGRGYAVSHVNSGEEALDE
                     MSRQPYELVVTDLRMPGISGIDLLHRVHELSPETRTILITAYGNDEVENESRRLNAAR
                     YFTKPFPIDEFTAAVQDVLESQTTDASLLSRERLERITQRLSDLRFEVGAQCILLADM
                     DGRLLAEIGLTDGLSPVELTELMSGGFDRLFRVARHLHEGHAFNLTYHEGARYDVYAA
                     NVGDRLFLTLVFDRRQGASRIGMVWLYTKRAIQDLLEFVSPSPRPANGGLPRTGPLR"
     gene            complement(4905..5882)
                     /gene="moaA"
                     /locus_tag="AC162G_16195"
     CDS             complement(4905..5882)
                     /gene="moaA"
                     /locus_tag="AC162G_16195"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR5077398.1"
                     /translation="MAYDSFGRNIHYLRISLTDKCNMRCVYCMPEEMVFRPTSELMSR
                     DECLRLVRLFVDLGVDKVRLTGGEPTVHSDLIPIVRGIRDLGVHRISMTTNGLRLAEL
                     ARPLKEAGLERVNVSVDTLNPEKFRRITRRGRLEEVMAGVVAAEAAGLTPLKINAVIA
                     RGYNEDEVAGLAALTLTHHWQVRYIEMMPFGEVAAFAQNSVVSQEEIMQLIEAELGAL
                     EPIDGGRLDGEARLYRLRGALAPIGFISTVTNPFCAACTRVRLTADGKLRLCLLRDDE
                     GDLLTPLRAGESDDELRTLIRNLIWRKPWGHGLPDGVIPIGRVMSQIGG"
     gene            6024..6455
                     /locus_tag="AC162G_16200"
     CDS             6024..6455
                     /locus_tag="AC162G_16200"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5077399.1"
                     /translation="MAEEISIRPARAEDTRAIKRMVRGAGLDPTLLRWENFLVAEKDG
                     NVVGIGQVKPYRGARELGSLVVLKAYRRQGIGAAIIRVLVEREPGALFLLCRDRLESY
                     YARFGFRRVGLRDLRGAVRLKYAFALLGRAAGVRVIAMRRG"
     gene            complement(6471..6986)
                     /locus_tag="AC162G_16205"
     CDS             complement(6471..6986)
                     /locus_tag="AC162G_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077400.1"
                     /translation="MQSTDRSTVVVAVVLIGLGALFLAFNLIPGLSIGQTWPVIFFLI
                     AGGFYLPAIIWPSERKSLAGLYIPGSIMLVLGLIFFFNTITGDWGAWAYLWTLIPGGV
                     GLGLALGSRIGEWGRDPRQVGIWMMVGSAIAFVFFGLLFGSVPIRTFGPLLLIAGGVL
                     LLARALRRPAQ"
BASE COUNT         1262 a         2330 c         2350 g         1298 t
ORIGIN      
        1 accatcctcg tcgtcgacga cgaccgccag atagccgaat tcctcgacga cctgcttcga
       61 cagttcggcc accgcgtcgt gaacgcctat gggggccagg aggcgcttga cgtgctcgcc
      121 tcgccggagg cgggcggcgt cgacctgatt ctgctcgacg tcctcttgcc ggacatgagc
      181 ggcaccgagg tgtgccagcg cgtcaaagcc tccccggcca ccgcacacat cccgatcatc
      241 atggtcaccg gcctggatcg gcctgccgac aagacgcgcg ggttggagct tggcgccgac
      301 gactacgtga cgaagccgtt cgatcccagg gagctggtcg cccgcgtggg cgcgatgctg
      361 cggctgcggc gcgccgagcg cgagctgcgc gcccgtaatc gcgccctggc tgcgctcaat
      421 gccgtggccg acacgatcgg ccgcgccgtc gagctgcccg acgtgttgaa caccgcactc
      481 gatcaggttc tggcgacgct cgacatggaa gccggcacga tcacgctgac gcactgggac
      541 ggcagccaga ttcccgccgc gcggcgctgg caggcgctcg acctgtcttc cggcgtggag
      601 gccgccgagc aggtcgcccg atccggcgcg ccggcgctcc tgacgctctc tgtccgggga
      661 cgcgatcacg cggcggcgtg cgtgccgctg cgtagtcgcg atcgggttgt cggtacgctg
      721 ctcgtcgccg gaaagcccgg gatcgattcg gccgggctgg agctgctcgg cgcgatcggc
      781 agccaggtcg gcggcgcgat cgagcgcggc cgcctgttcg acgccgcgca gcgccgcagc
      841 gacgatctgg cgatcctcaa cgacatcaca cgcgccgtca cgtcttcgct cgacgtcgac
      901 atggtgctag cgcgcgcgat gcgcagcatt cgcgaaatcc tgcgcgtcga ggccggatcg
      961 ctgatcctgg ccgacgagga tacgggcgag ctgcgctttc gcaagacgct caaccgcgag
     1021 caggagatga ttgtggacac gacgctgcgg cccggcgagg ggttgatcgg ccaggctgtc
     1081 gcgacgctcg atccgatcct gctcaacgac gcgccgagcg acgcgcgctt cgcgccgcac
     1141 ctggatcgca tcacgggcgt cgtcacgcgc ggcgcgctgg tcgcgcctct gatcgtcaaa
     1201 ggccgcgcga tcggggcgat cgaggtcatc aacaagctgg acggtccgtt cacacatgac
     1261 gatctggaga tgctccagtt cctggcggcg tcggtcgccg tcgccgtgga gaacgcgcgg
     1321 ctgtacggcg agctggccga gttcacgcgc cagctcgagc ggtcgcaaga gcagttgata
     1381 caggccgaga aggtcgccgc caccggtcgc ctggctgcgt cgatcgcgca cgagatcaat
     1441 aacccccttc aggcgatcca caactgcctg cacttggtca cccatcgtaa gatggagccg
     1501 gagaaggaag cgtattacct caacctggcg caggaagaag tgcagcgcct gatccacatc
     1561 gtgcagcgca cgctcgactt ctaccgccct tcgcaagggc gccatacgct cacggacgtc
     1621 aacaacgtga tcgaatcggt gctggcgctg gcgaacaaaa aagtggagca cgcgaaggtg
     1681 catgtgcacc gccgcctgtc ccccgacctg ccgaagctga acgccgtcgc cgaccagctc
     1741 acccaggtgt tcctgaacct gatcgtcaac gccgtcgaag cgatgcccgg cggcggcgac
     1801 ctcgcgatca ccacgctgca cgacgaaagc cacgtgcgcg tccgtatcag cgataccggc
     1861 cccggcctga atgccgagga agccaagcgc atcttcgagc cgttctatac cacgaagcgc
     1921 tccggcaccg gcctcgggct ggccgtgagc tacggcatca ttcagcgaca cggcggcgag
     1981 atcgccgtcg acagcgcgcc cggccgcggc gcgacgttca tcgtctcgct gccggtgaat
     2041 cgcgccggcg tcgcggtaga gaatgagccc cccgccattc gagtgtgagc acagccatgg
     2101 acgcgaacag ctcctttcac atcttgatcg tggacgacga ggccgccatg cgcgccacgc
     2161 tgtccgagat cctaggcggc gaaggctacc gggccagcgc ggtcgagagc ggcgaagcgg
     2221 cgctggacat cctgcgcgtg acgccgatcg acttgatgct gctcgacctg aagatggccg
     2281 gcctggacgg cctgcaggtg acggagacgg cgcagtcgat cagcccggac acggtgatcg
     2341 tcatgctcac cgcgcacggc acacttgaat cggcgatcac ggcaatgcgg cgcggcgcgc
     2401 acgattacct gctcaagccg gcgtccgtgc ccgagatcct ggcagccgtc gagcgcggcc
     2461 tgaggcaccg cgcgcaggcg ctgaagcagc gtgatctcat cacgctcatg cagcgcgcca
     2521 tccaggaact gcaagtgcct ggcagcggcg ccggcggcga gggcgccggc cccgaccggt
     2581 ttctgcaaat acgcggcgtc agcctggacc tgcagcggca cgtcgccaca gccaacggca
     2641 agctgctcga cctgacgctc accgagttca agctgctggc ttatctcatg cagcatcccg
     2701 accaggttat cgcgccgcgc gagctggtct cggcggtaca ggggtatgaa gccgacgagc
     2761 acgaagcgcg cgctatcatt cgggtccacg tgcggcgctt gcggcagaag atggagccgg
     2821 accccgataa tccagagtat gtgctcaacg tgcgcggcgt gggctacctg ttcgcgacga
     2881 cctcgccgtg acgccagact agtctactcg cctttccgaa tgtcgatatc gccgttgctg
     2941 gtcgtggcgg tgatcgagac atctgggttt tccccaacca cgccgtgcag ctcttcgtca
     3001 cgcgacgagc cggagcgcga caccgggaat tcggtcgaca cgtcgccgtt gctggtggat
     3061 gcgtcgatgc ggaactgggc gccggagggc agcgtgatct cgatgcggcc gttgctggtg
     3121 cggaagtcgt ggcttccttc ggccagcgac ccgcggaagt cgatctgtcc gttggacgtt
     3181 cgggcgtcga cgatggcgtc tcgcgcttcg acgtcgatgc ggccgttgct ggttgccagg
     3241 tcgagccgcc ctccgccgcc gtccacttcc agtgcgccgt tcgacgtgtc catcgcaata
     3301 tcgcctgtga cgccgttgga gcggatatgc ccgttgctgg tgtgcagatc gagtgacaca
     3361 ccggccggca cgctcagctc aatcgaagcc cctgaattgc ccataaacag gggcccgccc
     3421 gtgcggcggg cgacaatccg caccgtatcg ccttcctggg tcatcgtgac ttcgacattc
     3481 ttcaggttgt ccttcgcttc cgcctggctg aagcctgcac cgtgcttggt cacctcggcc
     3541 aggactttgg cctcggcgcc ggcggtcact tcgatgtggc cgttgtacgt ttcgacgacg
     3601 acgcgtggcg aggcgctcgt gtcaaacgac tgagccacga tttcctcggc ggtcacgttt
     3661 ccacccatgc agccgctcaa cgccagcgcc agtccggcga gcagtgtccc gatgaaagcg
     3721 ggtttcctcg tcgtcatgcc tcttctcctt tgccaagatg ggtgtctggt gattatccac
     3781 atcggcttgc cgtccgcgcc ctcgcgggca gcccgcccgc tgggaagcga gcgctgagcg
     3841 gcttgcgggc aatcatcaga ggtatcgatc ccagtatatt tcacaacagt agaaacggat
     3901 cagtcaccgg actggaatcg tatgtaagtc tccggtcgag cgtcgcatcg gtctaaggtc
     3961 ggatgagggg gtaacctaac cgtaacacag gggtaactcg ctcccgaaat gaaccgtgct
     4021 atcctgttgc cagtctctaa gagcgctctg gaatccgcac atgcctggca aacgcatact
     4081 gattgtggac gacgagacga aggtggggtt cttcctgaag gagaccctcg aatcgctggg
     4141 gcggggttat gccgtgtcgc acgtgaacag cggcgaggag gcgctcgacg agatgtcgcg
     4201 ccagccgtac gaactggtcg tgaccgatct gcgtatgcct ggaatcagcg gcatcgacct
     4261 gctgcatcgc gtccatgagc tttcgcctga gacgcggacg atcttgatca ctgcttacgg
     4321 gaacgacgag gtggagaacg aaagccggcg cttgaatgcc gcgcgttatt tcaccaagcc
     4381 cttccccatt gacgagttca ccgccgccgt gcaggacgtt ttggaaagcc agactacgga
     4441 cgcctccctg ttgagccggg aacgcctcga gcgcatcacg caacggctga gcgatttgcg
     4501 cttcgaggta ggagcgcagt gcatcctgct ggccgacatg gatggccggc tgctcgcgga
     4561 gatcggactc accgatggcc tgtcgccggt tgagttgacc gagctgatga gcggagggtt
     4621 cgaccgcctg ttccgggtcg cgcgccatct ccacgagggg cacgcattca acctgacgta
     4681 tcacgaaggc gcgcgttacg acgtttatgc ggctaacgtg ggcgaccgcc tgttcttgac
     4741 gctcgtcttc gatcgccggc agggcgccag ccggatcggt atggtgtggc tgtacacgaa
     4801 gcgcgcgatc caggatctgc tcgagttcgt ttcgccgtcg ccgcgccctg cgaacggcgg
     4861 ccttccgcgc actggcccgc ttcgctaggg agggttaggg tttgctatcc tccgatctgg
     4921 ctcatcaccc gcccgatggg gatgacgccg tccggcaggc cgtgtcccca cggctttcgc
     4981 cagatcaggt tgcgaatgag agtccttagc tcgtcgtcgc tctcgcctgc gcgcagcggc
     5041 gtaagcaggt caccctcgtc gtcgcgcagc aagcacagcc gcagcttgcc gtcggcggtg
     5101 aggcgcacgc gcgtgcacgc cgcgcagaat gggttggtga ccgtgctgat gaagccgatc
     5161 ggcgcgagcg ctccccgcag gcgatacagc cgcgcctcgc cgtccagccg gccgccatcg
     5221 attggttcga gcgctcccag ctcggcctcg atcagctgca tgatttcttc ttgcgacacg
     5281 acactgttct gggcgaacgc cgccacttcg ccgaacggca tcatctcgat gtagcgcact
     5341 tgccagtgat gagtgagggt gagcgcagcc aggccggcca cctcgtcttc gttgtagccg
     5401 cgtgcgatga cggcgttgat cttcagcggt gtcagacctg ccgcctcggc cgcgacgacc
     5461 cccgccatga cctcttcgag cctgccccgg cgtgtgatgc ggcgaaactt ctccggattc
     5521 agtgtgtcga cgctgacgtt tacgcgctca agcccggcct ccttcaaggg cctggccagt
     5581 tccgccagtc gcagcccgtt cgtcgtcatc gatattctat gcacacccag atcgcggatg
     5641 ccgcgcacga tcgggattaa gtcggaatgc acggtcggtt cgccgccggt caggcgcacc
     5701 ttgtcaaccc ccaggtcgac gaacaggcgc accaaacgca gacattcgtc gcgcgacatt
     5761 agctcggatg tcggccggaa caccatctct tccggcatgc agtaaacgca gcgcatgttg
     5821 catttgtcgg tcagggagat gcggaggtag tgaatgttgc gtccgaagga atcgtaggcc
     5881 atgtggaatc acccgttcgt ctttttcgac atagcgcttt gattgtatca ctggggcata
     5941 cgcctgtcaa taaagacgac ctctatcatc caattatggg gttttgacgc ctatctgggg
     6001 cgccaggtac aatggaagcc gtcatggctg aggagatctc aattcggccc gcgcgagcgg
     6061 aagacacccg cgccatcaag cgcatggtgc gcggcgcggg tctcgaccct acgttgctgc
     6121 gctgggaaaa cttcctggtc gccgagaagg atggcaatgt ggtcggcatc gggcaggtga
     6181 agccgtatcg cggcgctcgt gagctgggca gcctggtcgt cctgaaggct tatcggagac
     6241 agggcatcgg cgcggcgatc attcgtgtgc tggtcgagcg tgagccgggc gccctgtttc
     6301 tcctgtgccg agatcgcctg gaatcgtact acgcgcggtt cgggtttcgg cgcgtcgggc
     6361 tgcgtgacct gcgcggggcc gtccgcttga agtatgcctt cgcgctgctc gggcgcgccg
     6421 ccggcgtccg cgtcatcgcg atgagacgcg ggtgagatga ccggcttccg ttactgcgca
     6481 ggccgtctca acgcgcgcgc gagcagcagc acgccgccgg cgatcagcag cagtggcccg
     6541 aaggttctga tcggaacact gccgaaaagc aggccgaaga agacgaacgc gatggcgctg
     6601 ccgaccatca tccagattcc gacctgcctt gggtcgcggc cccattcgcc gatgcgcgag
     6661 ccgagcgcca gtcccaagcc gacgccgcct ggaatcagcg tccacaggta ggcccaggct
     6721 ccccagtcgc ccgtgatggt gttgaagaag aatatcagac ccaacaccag catgatcgag
     6781 ccggggatgt acaggccggc caggctcttg cgctccgagg gccagatgat cgccggtagg
     6841 taaaatccgc ccgcgatcag aaagaagatg acgggccacg tctggccgat gctcaggccc
     6901 ggaatgaggt tgaatgccag gaacagcgct cccaggccga tcaacacgac ggcgacgacg
     6961 acggtgctgc gatccgttga ctgcatgcta tggctcctga aagatgggat ggcgtgtgcc
     7021 gagaagagac ggccccatca tagccgattt gctcgtcgcc gtcatctgcc gatcggcgga
     7081 ttttcgtcag tcatttggcc tagaggaatt gacccggcct cgaaacggta taatagggct
     7141 gtggggagat agaaccttgg tgtcttcgtg tcttggtggt gtgcgaatcc cgggagtgcg
     7201 cggattgaga cagcggaggc aggaaagatg acccgcgttc
//
LOCUS       DBNHXB010000404         4668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2466904, whole genome shotgun sequence.
ACCESSION   DBNHXB010000404 DBNHXB010000000
VERSION     DBNHXB010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2466904"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..445)
                     /locus_tag="AC162G_16210"
     CDS             complement(137..445)
                     /locus_tag="AC162G_16210"
                     /inference="COORDINATES: protein motif:HMM:NF052065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7948 domain-containing protein"
                     /protein_id="HIR5077401.1"
                     /translation="MGLADTVRPSSRLAAAPRTHSPINSHTQKNSNPNPPIVGFDRLD
                     TTVSYFIGNDPSNWHTHVPVWGGVCYVDVYPGIDLEVTSLGGKWDWRPVARTLGTTER
                     "
     gene            complement(477..1889)
                     /locus_tag="AC162G_16215"
     CDS             complement(477..1889)
                     /locus_tag="AC162G_16215"
                     /inference="COORDINATES: protein motif:HMM:NF015052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HIR5077402.1"
                     /translation="MPALAVHSHFYQPPREDPFTGVMPVEPQAAPYHDFNEKVTAECY
                     RPLAERGVFERLSFNIGPTLMGWLARYAPDVYARITDADRSQVKRIGVGNALAQPAHH
                     TILPLARRRDKLTQVRWGIASFRHRFERQPRGMWLPEMAVDLETLDVLSEEGLSFTIL
                     SEQQVSGDLRAGAGPYRVRLPRGGYFALFVRDRDLSDAVAFSLPKLGDARMWTSEVLT
                     GRGGQLTVIASDGETFGHHHRQGIDFLEALLSGGDLITGRGKPGPYAVLSLADYLRRH
                     PPTIEVEVKERTAWSCWHGLDRWDAGCDCTPGDSSWKPALRSALDLLADEIDAMYLHE
                     TRRLVEQPWRLIDDSIDVVLGVTDMRTLVDECAKRVLKTGQLQRIGHLVKAQLHRQRM
                     FTSCGWFFDDLDRFEPRYVVANAARAIQLCCQATGVDLGPVFRETLTSVRSGASGLTA
                     AEVYDKIVARAQAAAPQTGH"
     gene            complement(1894..4086)
                     /locus_tag="AC162G_16220"
     CDS             complement(1894..4086)
                     /locus_tag="AC162G_16220"
                     /inference="COORDINATES: protein motif:HMM:NF020660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HIR5077403.1"
                     /translation="MADKLYLCLVLHNHQPVGNFDFVFEEAVAHAYEPLVDALERHPG
                     VRLTMHWSGSLRDWLHAHRPDLLARIRTLVGHGQVEPLGGGYYEPVLVALPDADKLGQ
                     LDKLTETVQQDFGVRPRGAWLAERVWEPHLPKFFAEAGLEYTLLDDTHFAQAGYSEDD
                     LYGTYVTEEQGYTLRVFVSSQHLRQAIPWSPVDQIMNWLRGLAEKPLAPGAPPRLVAV
                     AEDGEKLGLWPGTDTLCWGDGKTGGWVESFFTALERSADWLTLLTLDDATRILPPLGR
                     AYLPAGSYEEMTVWSLPAVRAQELAALKQQLQAAGREDILRYVKGGTWRSFMTKYPEV
                     NTLHKRIVFASRKVRQIKKAALRAKALDHLWASQCDCPYWHGVFGGVYLFHIREAAAR
                     HLIEAEAIADRALHAGETWSDVTTSDIDCDGFAEIVLASDTQQVMVSLGQGGSIVAWD
                     WRATKTNLLNVLSRRREAYHASLIEAAANGTLVVAGQPPSLPPQAGGAGGGATVRAKE
                     AGLEERLIYDWYRRASLIDHVFAPDASLDAFYRSTYDELGDFANQPYTAKSSRPKGSV
                     VLSLARDGGIHLADRRMPLRVEKKIGLHPGESRLAVDYAVTNLGDRAVKARFGVETNW
                     GISGGDSSEGAYLVWPGGTLERLNRVCETKSAGDVAIVHEWIGRVVVHAGQAATWWQF
                     PIETISNSEAGFERVYQGLSLTAHWPLDLEPGGVWRLSLSFTLLPAVD"
     gene            complement(4105..>4668)
                     /locus_tag="AC162G_16225"
     CDS             complement(4105..>4668)
                     /locus_tag="AC162G_16225"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlgC family sugar phosphate
                     nucleotidyltransferase"
                     /protein_id="HIR5077404.1"
                     /translation="PFTDYWVDVGTVQAYWEAHMDLLVDDPQLNLLDRNWVIHTVSEE
                     RPPVNIRTGAQVSHSLITDGCVIEGAVEYSVLSPGVRVARGAVVRNSIVLTDCDIRAG
                     AVIDRSIIDKQTVVGENAHVGQGADYSPNPLGDLVTGITLVGKNARIRPGAKIGRNCV
                     ISSDVVESDFSELVVPSGTTVGRLPKQ"
BASE COUNT          799 a         1573 c         1495 g          801 t
ORIGIN      
        1 aggaaacaac agttttgtcg cctgtacgtc tgagtgctgc gcgatctgtc atctaacgtc
       61 ccacccatca gcacccgccg gcactatcgc gcgcggcctt ctccccttcc atttccattg
      121 gcagaccgtc ctcgcgtcat cgttcggtcg tccccagcgt gcgtgcgacc gggcgccagt
      181 cccacttccc gcccaggctg gttacttcca ggtcaatgcc ggggtataca tccacgtagc
      241 acacgccacc ccaaacaggc acatgagtat gccagttgct gggatcgttg ccgatgaagt
      301 acgacacagt cgtatccaat cggtcaaagc ccacgattgg cggattgggg ttggagtttt
      361 tctgcgtgtg tgagttgatg ggggaatgag tgcgcggtgc ggctgcaagg cgcgaggatg
      421 gacggacagt gtcagcaagg cccatggttt cgctggctcg cctgcgccag gacgtatcaa
      481 tggccggtct gaggtgccgc tgcttgtgca cgcgccacaa tcttgtcgta cacctcggcg
      541 gcggtgagtc ccgaggcgcc gctgcgcacg gaggtgagcg tctcgcgaaa gaccggcccg
      601 agatcgacgc cggttgcctg gcagcacaac tggatcgcgc gcgcggcgtt ggccacgacg
      661 tagcgcggct cgaagcgatc caggtcgtcg aagaaccagc cgcagctcgt gaacatgcgc
      721 tgccggtgca gttgtgcttt gactaagtgc ccgatgcgct gcaactggcc ggttttcagc
      781 acgcgcttgg cgcactcgtc gacgagggtg cgcatgtcgg tgacgcccaa caccacgtcg
      841 atcgaatcgt cgatcaatcg ccacggctgc tcgaccaggc ggcgcgtctc gtgaaggtac
      901 atggcgtcga tctcgtcggc cagcaggtcg agcgccgagc gcagcgccgg tttccacgat
      961 gagtcgccgg gcgtgcagtc acagcccgca tcccagcggt ccaggccatg ccagcacgac
     1021 caggcggtgc gctctttgac ttccacctcg attgtcggcg ggtgccgccg taaatagtcg
     1081 gcgagcgaca gcaccgcgta ggggccgggc ttgcccctgc ccgtgatcaa atcgccgcct
     1141 gacaagagcg cctccaggaa atcgatcccc tgccggtgat gatgcccgaa ggtctcgccg
     1201 tcagaggcga tcacggtaag ctgaccgccc cgtcccgtca gcacttcgct cgtccacata
     1261 cgggcgtcgc ccagcttcgg caggctgaac gcaacggcgt cggagagatc gcggtcacgc
     1321 acgaacaggg cgaaatagcc gccgcgcggc aggcgcacgc ggtacggtcc cgccccggcg
     1381 cgcaggtcgc cgctcacctg ctgctcgctc aagatcgtga acgacaggcc ctcttcggac
     1441 agcacgtcga gcgtttccag atcgacggcc atctcgggca gccacatacc gcgcggctgc
     1501 ctctcgaagc ggtgccggaa ggaagcgatc ccccagcgca cctgcgtcag cttgtcgcga
     1561 cggcgagcca gcggcaggat cgtgtgatgc gccggctgcg cgagcgcgtt acccacgccg
     1621 atccgcttca cctggctgcg atccgcgtcc gtgatgcgcg cgtagacgtc cggcgcgtag
     1681 cgggccagcc agcccatcag cgtcgggccg atgttgaacg acagacgctc gaagacgcca
     1741 cgctcggcga gcgggcgata gcattcggcc gtgactttct cgttgaagtc gtggtaaggc
     1801 gcggcctgcg gctcgaccgg catgacgccc gtgaaaggat cctcgcgcgg gggctgatag
     1861 aagtgactgt gaacggcgag cgccggcatg tggttaatcg accgcaggca atagcgtgaa
     1921 ggacagggat agcctccaca cgccgcccgg ctccagatcg agcggccaat gcgcggtgag
     1981 cgacagaccc tgatacacgc gctcgaaacc ggcttcggaa ttggagatcg tctcgatcgg
     2041 gaactgccac cacgtggccg cctggccggc gtggacgacg acgcgcccga tccattcgtg
     2101 gacgatcgcg acgtcgccgg cgctcttcgt ctcgcacacg cggttcaggc gctcgagcgt
     2161 gccgcccggc cacaccaggt acgcgccttc gctgctgtcg ccgccggaga tgccccagtt
     2221 ggtctcgacg ccgaaccgcg ccttcaccgc tctgtcgccg agattcgtga cggcgtagtc
     2281 cacagccagc cggctttcgc ccggatgcag gccgatcttt ttctcgaccc tgagtggcat
     2341 cctgcgatcg gcaaggtgga ttccgccatc gcgcgcgagt gacaatacga cggatccttt
     2401 cgggcgggag gatttcgccg tgtagggttg attcgcgaag tcgcccagct cgtcgtaggt
     2461 cgagcggtaa aacgcgtcga gactcgcatc cggcgcgaag acgtgatcaa tcaggctggc
     2521 gcggcgatac cagtcgtaga tcaggcgctc ttccagaccg gcttccttcg cgcgcacggt
     2581 cgccccccct ccggctcccc ccgcttgcgg ggggagagaa ggcggctgcc cggcgacgac
     2641 gagcgtgccg ttggccgccg cctcgatgag gctggcgtgg tatgcttcgc gccggcgcga
     2701 cagcacgttg aggagattcg tcttcgtcgc gcgccagtcc caggccacga tcgacccgcc
     2761 ctgccccaac gacaccatca cctgttgcgt gtcactggcg agcacgatct ccgcgaagcc
     2821 gtcgcagtcg atgtcgctcg tcgtcacatc cgaccacgtc tcgccggcat gcagcgcgcg
     2881 gtcggcgatc gcctcggcct cgatcaggtg ccgcgcagcc gcctcccgaa tgtgaaacag
     2941 atacacgccg ccgaacacgc cgtgccagta cgggcagtcg cattggctgg cccacaggtg
     3001 gtcgagcgcc ttggcgcgga gcgccgcctt cttaatctgg cgcactttcc gactggcgaa
     3061 gacgatgcgc ttgtgcagcg tgttgacctc ggggtacttg gtcatgaacg agcgccacgt
     3121 gccgcctttc acgtaccgca gaatatcctc ccggccggcc gcctgcaact gctgcttgag
     3181 cgcggccagc tcctgcgccc gcacagcagg cagtgaccag accgtcatct cttcgtacga
     3241 gccggcgggc agatacgccc gaccgagcgg cggcagtatg cgcgttgcat cgtcgagcgt
     3301 cagaagcgtc agccaatcgg cgctgcgctc gagcgcggtg aaaaacgact cgacccagcc
     3361 cccggtcttg ccgtcgcccc agcacaaggt atcggtgccg ggccatagcc cgagcttctc
     3421 accatcctcg gcaacggcga cgagccgcgg cggcgcgccc ggcgcaagcg gcttctccgc
     3481 cagcccgcgc agccagttca tgatctgatc gaccggtgac cagggtatgg cctggcgcaa
     3541 atgctgagac gatacgaata cccgcagggt gtagccctgc tcttcggtca cgtaggtgcc
     3601 gtatagatcg tcttcggaat agccggcttg cgcaaagtgc gtgtcatcga gcagcgtgta
     3661 ttccaggccg gcctcggcga agaacttggg caggtgcggc tcccagaccc gctcggccag
     3721 ccacgcgccg cgcgggcgga cgccgaagtc ttgctgaacc gtctccgtca gcttgtccaa
     3781 ctggccgagc ttgtccgcgt cgggcagcgc gacgagcacc ggctcgtaat atccgccgcc
     3841 cagtggctcg acctgcccgt gaccgaccag cgtgcgaatc cgcgcgagca agtcgggccg
     3901 gtgcgcgtgc agccagtcgc gcagcgatcc cgaccagtgc atcgtcagcc gcacgcccgg
     3961 atggcgctcc agcgcgtcga cgagcggctc gtaggcatgg gcgacggcct cctcgaagac
     4021 gaaatcgaag ttcccgaccg gctgatgatt gtgtaagaca aggcagaggt agagtttgtc
     4081 ggccatggcg cgatcgaacg acactcattg cttcggcaag cgcccaacgg tcgtgccgct
     4141 gggcaccacc agctccgaaa aatcgctttc gacgacgtcg ctgctgatca cgcagttccg
     4201 gccaatcttc gcgcctggcc tgatgcgcgc attcttgccg accagtgtga tccccgtcac
     4261 caggtcgccc aacggattgg ggctgtagtc ggcgccctgg ccgacgtgtg cgttttcgcc
     4321 gacgacggtt tgcttgtcga tgatgctgcg atcgatgacg gcgccggccc gaatatcgca
     4381 atcggtcagc acgatcgaat tgcgcacaac cgcgccgcgc gcgacgcgca cgcccggcga
     4441 cagcacgctg tactcgaccg ccccttcgat cacgcagccg tcggtgatca ggctgtgcga
     4501 cacctgcgcg ccggtgcgga tgttgaccgg cggacgctcc tcggacacgg tgtgaatgac
     4561 ccagttgcga tcgagcagat tgagctgcgg atcgtcgacg agcagatcca tgtgcgcttc
     4621 ccaatacgcc tgcaccgttc cgacgtctac ccagtagtcg gtgaaggg
//
LOCUS       DBNHXB010000405         3918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2133303, whole genome shotgun sequence.
ACCESSION   DBNHXB010000405 DBNHXB010000000
VERSION     DBNHXB010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2133303"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="AC162G_16230"
     CDS             <1..843
                     /locus_tag="AC162G_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5077405.1"
                     /translation="RDEDQTIVRADVDGARKLSGIPAENLSVFKVSPDETLIAWTSNP
                     WDQTPPQSELWIANLDGSNARKVATSTTDASSELFVPTPYRWTDDGKLLYVETPTGIG
                     GYILYRAYSSLHVFDPGTGEIRPLYQPEDKYSVCLSDVASDLSRVGFGCGEGGAGRVS
                     ILTLADGKTTPVPDLAEQGQAGSTRFSPSGAWLAYAIARGDPERERGQVAVVPTDLSA
                     APTVIASQDGGFYDVINWIDEDTLLLFLTREGQGSVWRIGHDGSDPMQLATGSYVVGM
                     LANK"
     gene            1001..1771
                     /locus_tag="AC162G_16235"
     CDS             1001..1771
                     /locus_tag="AC162G_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077406.1"
                     /translation="MPTSAPEPAGLRPMQVYNVEVQIGVGSPIPVDAFISAELPDTCA
                     QLAEVRQQLEDFKFEITLSVMPGTGEECFRDTLPFRMVVPLNMVNLPDGTYTVDVNGV
                     STTFTWPSAPTVTPEPIISTYRNTLAGFELDYPVGWLLDEAGDTDILWSQRPTGPGTG
                     GVPSDVVKIDVSSEPNTTMTLEELVARQKQGIASDQILSEEELELPSGLRAVRMQVSV
                     LGDSVTLMAVINGHPVVVTGFGDLSRFDEVVRTLRPSQ"
     gene            complement(2030..2117)
                     /locus_tag="AC162G_16240"
     tRNA            complement(2030..2117)
                     /locus_tag="AC162G_16240"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2081..2083),aa:Ser,seq:cga)
     gene            complement(2272..3030)
                     /locus_tag="AC162G_16245"
     CDS             complement(2272..3030)
                     /locus_tag="AC162G_16245"
                     /inference="COORDINATES: protein motif:HMM:NF024664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HIR5077407.1"
                     /translation="MPTPFPGMDPYLERPGVWEDIHTPLILEIANLLEPRIQPLFRVA
                     VERRAYQTMLWEPETPAAEPETLLAPHDAAPGGVKPRACELPLPQQVIEHFLEIRDVI
                     SGEAVTIIEVLSLDNKLTPEGRQEYERQRRAVLGGPANLVEIDLLRAGDPFPMRVEGQ
                     SVRSDYRIVVSRAPFRPHADVYLFGVRDAIPDIPIPVQRGKTEPMLPLNEILHNLYDR
                     AGPDLVIDYRQPPDPPLSAEDAEWANPLLQRFRS"
     gene            complement(3045..>3918)
                     /locus_tag="AC162G_16250"
     CDS             complement(3045..>3918)
                     /locus_tag="AC162G_16250"
                     /inference="COORDINATES: protein motif:HMM:NF022449.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR5077408.1"
                     /translation="GRPRRIVFSGDLGRKNLPILRDPEPVDHADVLITEGTYGGRFHK
                     PIEDAKDDLAEVISRTVERGGLLLVPAFAVGRTQELVYDLHQLCEAKRLPEIPIFVDS
                     PLATNVTEIFRRHPDVYDSETLDFMADLDGRDPFGFSRLTYVRDAEASKALNDRDEPA
                     VLIAPSGMCEGGRILHHLRHHVGDARNAVLFVGFQAEHTLGRQLLDGRKRVKIFGEEH
                     DVGAEIVRLEGYSAHGDHNDLVGFARSISPRPGQTFIVHAELEAAEALRAELAGTGFQ
                     NVAIPDRGDSFEVQ"
BASE COUNT          765 a         1188 c         1225 g          740 t
ORIGIN      
        1 cgcgatgaag accagaccat cgtccgcgcg gatgtggatg gcgctcgaaa actctctggc
       61 attcccgcag agaacctgag tgttttcaag gtgtcacctg atgaaacgtt gattgcgtgg
      121 acgagcaatc catgggatca gacgccgccg cagagtgagt tatggatcgc caacctggat
      181 ggctcaaatg cacgcaaggt cgcgacttcg accacagacg ccagcagtga attgttcgtg
      241 cccacgcctt atcgctggac ggacgatggc aagctgttgt acgtcgaaac gccgaccggc
      301 ataggtggct acattcttta tcgggcgtac agtagtctgc acgtgttcga tccgggaacg
      361 ggcgaaatca ggccgctcta tcagccggaa gataagtaca gcgtgtgcct gagcgatgtg
      421 gcgagcgatc tatcgcgtgt agggtttggc tgcggcgaag gcggcgcagg cagggtcagc
      481 atcctcacgc tggccgatgg caagaccact cccgtgcccg atctggccga acagggtcag
      541 gctggaagca cacgcttctc gccatccggc gcgtggctgg cctacgcgat cgcgcgcggc
      601 gatccggaaa gagagcgtgg acaggtggcc gtcgttccta ccgacttaag cgcggcaccc
      661 accgtcatcg cgtcacagga tggcggattc tacgatgtaa ttaactggat cgatgaagat
      721 acactgctcc tctttctgac gcgcgagggt caaggctcgg tctggcgcat cggccatgac
      781 ggcagcgatc caatgcagct tgccaccggc agctacgtcg tagggatgct tgccaacaaa
      841 tgagccggga acctgaagag agatttgggc gtcccaatca cagtattatt caaagccgtg
      901 aggaggcatg gaaatgttgc gttcgttcac aacaccttcc gttctcgttg tgctagtgat
      961 tgcgctagcc gcttgcgcac cggctgcggc gccgaccgcg atgccgacca gcgcaccgga
     1021 gccggccggc cttcgtccca tgcaagtgta taacgtcgaa gtccaaatcg gtgtcggctc
     1081 gcccatcccg gtcgacgcgt tcatcagcgc cgagctgcct gacacctgcg ctcagctcgc
     1141 cgaggtcagg cagcaactgg aggatttcaa gttcgagatc acgctctcgg tgatgccggg
     1201 gacgggtgaa gaatgttttc gagatacgct gccattcagg atggtcgtgc cgctcaacat
     1261 ggtgaatctg ccggatggca catacaccgt cgacgtcaat ggcgtgagca cgacgttcac
     1321 ttggccatcg gcgccgacgg tcacgcctga gccgatcatc tcgacgtatc ggaacaccct
     1381 ggcaggcttc gagctcgact atccggtggg ctggctgctc gacgaggcgg gcgacaccga
     1441 cattctgtgg tcgcagcgcc ccaccgggcc gggtaccggc ggcgtgccga gcgacgttgt
     1501 gaagatcgat gtatcgagcg agcctaacac gacgatgacg ctggaagaac tcgttgcgcg
     1561 gcaaaagcag ggcatcgcca gcgatcagat tctttctgaa gaggaattgg aactacccag
     1621 tggcctgcgc gctgtgcgaa tgcaggtatc agtcttgggc gacagcgtga cgctgatggc
     1681 agtcatcaac ggacatcccg tcgtcgtcac cggcttcggc gacttgagcc gcttcgacga
     1741 ggtggtgcgc actctgcggc cgagtcaata gaagagcgcg ggtgattgca cacatgtacc
     1801 ggtttgcccg ccgacacgcg aaggtgattt cagctcccat tttcgtgcat atctctgcaa
     1861 gctcgaatac gtcactctaa gccgtcttgc ggcctcgtga taactacccg cttcctgaat
     1921 acgctgggcg agttcttgtc gatctaggct agcgagtttg actacgcgtg ggggcctgga
     1981 tcaagcacaa cctaaaacaa aagactcttg ccgatcaaga gtctgttagc ggaggggctg
     2041 ggattcgaac ccagggtgac ccaaagagcc acaacggttt tcgagaccgc cccaatcgtc
     2101 cactctggca cccctccgag tgaaccagcg tgattataac atagcgcggc ctccaaccac
     2161 aaccaaatca tcggacgcgg atttatgcgg atcaacgcag atagaatcag aatcagcgta
     2221 atccgcgatc atccgcgtcc gaaaaactag attgcgaatg ctctgccaag attacgacct
     2281 gaatctttgc agcaacgggt tcgcccattc ggcgtcttca gcagaaagcg gcggatcggg
     2341 cggttgccgg taatcgatga ccagatcggg gccggcccga tcgtagagat tgtggagtat
     2401 ctcgttcaga ggcagcatcg gctcagtctt gccccgctgc accgggatag gaatatcggg
     2461 aatggcatcc cgcacgccga acaagtacac gtcggcgtgc gggcggaagg gtgcgcggct
     2521 gacgacgatg cggtaatcgc tccgcacgct ctggccttcc acacgcatgg ggaagggatc
     2581 gcccgcccgc agcaggtcga tttccaccag gttggccggg cctcccaaca ccgcgcgtcg
     2641 ctggcgctcg tattcctgcc ggccctcggg cgtcagcttg ttgtccagtg acaagacctc
     2701 gatgatcgtc acggcctcgc cgctgatcac gtcgcgaatc tcgaggaagt gctcgatcac
     2761 ttgctggggc agcggcagct cgcaggcacg gggtttcaca ccgcccggcg cggcgtcgtg
     2821 gggcgcaagc agcgtctccg gctcggccgc gggcgtctca ggctcccaaa gcatcgtctg
     2881 gtacgcgcgg cgctcgacgg caacccggaa aagcggctga atcctgggct cgagcaagtt
     2941 agcgatttcc aggatgagcg gcgtgtgaat gtcttcccac actccggggc gctccaggta
     3001 cggatccatt ccgggaaacg gcgtaggcat agttgctcct tcgattactg gacttcgaaa
     3061 ctgtcgcccc gatcggggat cgccacgttc tggaagcccg taccggccag ctccgcccgc
     3121 agcgcctccg ctgcttccag ttcggcgtgc acgataaacg tctggccggg acgcggcgaa
     3181 atgctgcgcg cgaaaccaac cagatcgttg tgatcgccgt gggcgctgta gccctccagt
     3241 ctcacgatct ccgcacccac gtcgtgctcc tcgccgaata tcttcacgcg cttgcggccg
     3301 tccagcaact ggcggcccag ggtgtgctcc gcctggaagc cgacgaacag cacggcgttg
     3361 cgcgcgtcgc cgacgtggtg gcggagatgg tgcaggatgc ggcccccttc gcacatgccc
     3421 gacggcgcga tgagcaccgc cggctcgtcc cggtcgttca acgccttcga cgcctcggcg
     3481 tcgcgcacgt acgtcaggcg cgaaaagccg aagggatcgc gcccgtcgag gtccgccatg
     3541 aagtccagtg tctcgctgtc gtacacgtcg gggtggcggc ggaaaatctc ggtaacgttg
     3601 gttgccagcg ggctatcgac gaagatgggt atctccggca agcgtttcgc ctcgcacaac
     3661 tgatggagat cgtagaccag ctcttgcgtg cggccgacgg cgaaggctgg caccaggagc
     3721 aggccgccgc gctcgacggt gcgtgagatc acttcggcga gatcgtcctt cgcgtcttcg
     3781 atcggcttgt gaaagcggcc gccgtaggtc ccttccgtga tcagcacgtc ggcgtgatcg
     3841 accggctccg ggtcgcgcaa gatcggcagg ttcttgcggc ccaggtcgcc gctgaacacg
     3901 atgcggcgcg gccggccg
//
LOCUS       DBNHXB010000406         9685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_959467, whole genome shotgun sequence.
ACCESSION   DBNHXB010000406 DBNHXB010000000
VERSION     DBNHXB010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9685
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_959467"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="AC162G_16255"
     CDS             complement(<1..1423)
                     /locus_tag="AC162G_16255"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HIR5077409.1"
                     /translation="MTERIAERIIESRRELMKSLPAPERAADAAEGGCGVLGFASSIP
                     VAGRHVLTASRQMHNRGNGKGGGIAMAGLDPAHMRVDAETLRTHTLLQIAVLDPSARE
                     EVEREFILPHFDVAQAYEIDRVDDYREIGGLEIRPPDVWRYFVRVKPAVLQRFAEEKR
                     LEIGDWRAVEDEFVYQNSFRLNTKFYASLGDKRAFVLCHGRDLIVLKIVGYAEQVAEY
                     YRLPDQTAHVWISHQRYPTKGRVWHPGGAHPFIGLNEALVHNGDFANYHAVSEYLRQR
                     NIGQLFLTDTEVSVQLFDLWDRVYRYPLEVTLEAMAPTTEYDFAMLPPEKQELYRTVQ
                     AAHMHGSPDGPWFFIVARSKPDEGASELIGITDTSMLRPQVFALFENEGAIESATNPA
                     ERGASGQRISESASRQAGESADRPTNPRVQIGLIASERQAINACLRSLASEDERFQPI
                     ADKYWVARGGSHTDGGSFRFAVSR"
     gene            complement(1452..1937)
                     /locus_tag="AC162G_16260"
     CDS             complement(1452..1937)
                     /locus_tag="AC162G_16260"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5077410.1"
                     /translation="MTREDRLTIIESYGKAHAELVEALRQFPREMWTFKPSPDRWSIH
                     EIVIHIADSEANSYVRCRRLVAEPGESVMAYDEMRWADALRYHDQPTEEAVELFKWLR
                     LRSYRLIKSLPEAVWSNTVLHPENGVMTMDDWLDVYERHVREHVEQMRANYANWTKVS
                     Q"
     gene            complement(1950..4451)
                     /locus_tag="AC162G_16265"
     CDS             complement(1950..4451)
                     /locus_tag="AC162G_16265"
                     /inference="COORDINATES: protein motif:HMM:NF013784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR5077411.1"
                     /translation="MKPHSRYHIDTEAAPMPLAYPAQFLLTEGEAYVKYGHPVFAGTS
                     QRGDEYWTAEIITQTWYQAESGRIPISGAGYGGKFGGDGFEGIWLDMSEIVRPTRDGI
                     HGRETISTQVDIGGGVSSLSFDEHGRLQSALPPSLPIPIPVVFNPLPIPLPGRGAIRA
                     LASAAADLGTLSIIDPADLSDDLFSNLQSPTSNLQSLSPRISTHNFDAVPDAWQPCYL
                     EVEEEREMARALLISRCRERWPDAVIAVRVPFSLDAPQQVESLVRDGVPVIHLFGDDM
                     GVDLSGRHLVDAIRAVHTHLVSQALRDQARLIVSGGIAAADHVAKAIACGADAVAVDF
                     VLQIAWGCALWADKGACPVEAKEIDADWGRQRVVNLMNAWRDQLLEVLGAMGMREMRR
                     LRGEVGRTIWHRAEDAAFKSMLGGSAPVKEYRRLPEPEGAEGDFRWTQALILSSHQQA
                     RTGEPPTGEYEYRVGRSDGGFDRLAFKLELELGVGEVPLADPAHFDFRVKLNRRADER
                     PALELPIPWYGAGMSYGSVGLPVMLARAKAAQHLGIFSSTGEGGYPEELEPHADHIIT
                     QVATGLFGVREDTLQRAPIIEIKYAQGAKPGLGGHLLGNKNTDSVARLREAVQGTSLF
                     SPFPFHSVYSVEDHKKHVDWLKQVNPRALISVKVSTPTDVDMVAVGSYYAGAHILQLD
                     GAYGGTGAAPEIAKKNIAMPIEYALPKVHRFLEQEGIRDEVTLMASGGIRTAYDVAKA
                     IALGADGCIIGTAELVAIGCIRIAQCEKGLGCPFGITTTDPEHAKKIDALIGWKKIVN
                     LYTSWLWQLAGILDRLGMKSIAELRGRSDALVYLE"
     gene            complement(4965..5390)
                     /locus_tag="AC162G_16270"
     CDS             complement(4965..5390)
                     /locus_tag="AC162G_16270"
                     /inference="COORDINATES: protein motif:HMM:NF016763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR5077412.1"
                     /translation="MKESGMIVRGKVINGWLGRWLPVAAWMAFIFFLSAQPSLPSPEE
                     DWLEELLNIAGHFTVYVVLALLVSRATAPDGHLSKWNKILVIGWCAAYALSDEWHQSF
                     VPGRDASVFDLAVDVLGMLGGLMVYVSRRPNKPVDDVPV"
     gene            complement(5407..6582)
                     /locus_tag="AC162G_16275"
     CDS             complement(5407..6582)
                     /locus_tag="AC162G_16275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5077413.1"
                     /translation="MRIVMIGPFGLKPKRTMAVRALPMARALAARGHAVTMILPPWAN
                     PEDSGRSDEDGGVRIENIELPPHLPLLFHVRTTRRLVRRALALEPDIVHCFKPKAYAG
                     LAAWWLWQLKGLGLSRVRLIVDTDDWEGAGGWNDRERYSWAQKRIFAWQEQWGLRHCD
                     ALTVASRALESIVWSMGVSPSRVFYVPNGVEVGSWKLEVGEAASKFQTPTVLLYSRFF
                     EFKIERVLGVFSRVLELNPEARLLVVGKGLFGEEKSLIDGAHQRGWTDRIEYLGWVEP
                     AELPDVFARAGVAIYPFDDTLLNRTKCAVKLIDLLAAGVPVVADAVGQNVEYIRHNET
                     GFLSPSGDVEAMAGAVVELLNERRRARILGGAAAKDVRERFGWDRLVESVERAYAVG"
     gene            6774..8645
                     /locus_tag="AC162G_16280"
     CDS             6774..8645
                     /locus_tag="AC162G_16280"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR5077414.1"
                     /translation="MPYTHAGKYIRIDLSTGKHTVEPISDDDVKSYLLGSGYAARLFY
                     EEMDPSIDPLDPRATLYIFNGLLSGTFAPTGCRSSWCGRSPLTGIWNEANLGGHFGAE
                     LRFAGYDGIVITGKAPRPSYLYINGITNTIEVRDATHLWGKDTFEVHDQLLKETDEKA
                     RPAVIGIAGERLVKIAGVAAGGHSHTRMAARGGMGALMGSKNLKAIVARGKDKPQYAD
                     AKGFHATVKESNHYIMDHSAAEALHLAGTAGGHPTTDKFGDNAIMNWRGGNWYDGTVK
                     TSGKAIADTIFTKHTFCHACPIGCGKAVEVKDGRYAGVWGEGPEYETLCGFGSNLQCD
                     DLNAIAAMNDLCNRYAIDTISTSGVLAFAFEAYEKGAITKDDTGGVELRWGDADAIMA
                     MIHKIGRREDVGDTLAEGVREAARRIGQGSEAYAVHVKGLEVPYHDPRGFVSMAANYA
                     TASRGACHLEALTYWRGVGIEWPGWEESEAQDWIERKRFDSSIGAQTAISFQNYMTTY
                     NPLGLCKFITKGSFTPAQTAELVNKAMGWSWTGHDVLDVGARLFNFKRLINNRLGIER
                     KDDVLPVRLTTEPRPTGTAAGVLPDMDVMLPEYYRLRKWDENGVPTKEAMLPWANNP"
     gene            8722..9243
                     /locus_tag="AC162G_16285"
     CDS             8722..9243
                     /locus_tag="AC162G_16285"
                     /inference="COORDINATES: protein motif:HMM:NF047646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase"
                     /protein_id="HIR5077415.1"
                     /translation="MGRLTHIYEPGMTYLITTITHRRERLFEDSRYARTAHEDIAFYA
                     RKFGMISVAHVLMPDHVHWIMHPLPEGFERFTREEQAKGKRSKYGHAPERFYLSKILE
                     DYKRHTAHAINEYRDIPNAQVWQEGFRDDALRTPQVIRGAVQYVLLNPVKAGLTERSE
                     DYPYLAWNADWLA"
     gene            9450..>9685
                     /locus_tag="AC162G_16290"
     CDS             9450..>9685
                     /locus_tag="AC162G_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5077416.1"
                     /translation="MPNTMSAEEMVRLSKEYTLWSWSAQAAVNPIPMVRGQGIYFWDA
                     DGKRYVDFNSQLMCVNIGHGDRRVIEAIKQQAEEL"
BASE COUNT         1879 a         3100 c         2948 g         1758 t
ORIGIN      
        1 ctcgcgacac ggcgaaacgg aacgatccgc cgtcggtgtg agacccgccg cgcgccaccc
       61 aatacttgtc cgcgatcggt tgaaatcgtt cgtcttctga cgccagcgat ctcaagcacg
      121 cgttaatcgc ttgccgttca gacgcgataa gcccgatctg gacgcgcggg ttggttggtc
      181 ggtcggctga ttcaccggct tgccggctcg ccgattcgct gattcgttga ccgcttgcgc
      241 cccgctccgc ggggttcgtt gcagattcga tggcaccctc attctcgaat aatgcaaaca
      301 cctgcggacg aagcatcgac gtatcggtga tgccgatcaa ctccgaggca ccctcgtcgg
      361 gcttcgaacg ggcgacgatg aagaaccatg gcccgtcggg cgagccgtgc atgtgcgccg
      421 cctgtacggt gcgatacagt tcctgcttct ccggcggcag catggcgaaa tcgtactcgg
      481 tcgtcggagc catcgcctcg agcgtcactt cgagcgggta gcggtacacg cgatcccaca
      541 ggtcgaacaa ctgcacactc acctcggtgt cggtcaggaa caactggccg atgttgcgct
      601 ggcgcaggta ctcgctcacg gcatgatagt tggcgaagtc gccgttgtgc acgagcgctt
      661 cgttcaggcc gatgaacgga tgcgcgccgc ctgggtgcca cacgcggccc ttggtcggat
      721 agcgttggtg ggagatccat acgtgcgcgg tctgatcggg cagccgataa tactcagcca
      781 cctgctcggc gtacccgacg atcttcagca cgatcagatc gcgcccgtgg cacagcacga
      841 aggcgcgctt gtcgcccagg ctggcgtaga acttcgtgtt cagccgaaaa gagttctggt
      901 aaacgaattc atcctcgacc gcccgccaat ctccaatctc taatctcttc tcttccgcga
      961 agcgctgcag cacagccggc ttcactcgca cgaagtaccg ccacacgtcc ggcggcctaa
     1021 tctctaatcc accaatctct ctgtagtcgt caacccgatc gatttcgtac gcctgcgcca
     1081 cgtcgaagtg aggcaggata aactcgcgct cgacctcttc gcgcgcagac ggatcgagca
     1141 cggcgatctg gagcagggtg tgcgtgcgca gcgtctcggc atcgacgcgc atgtgggccg
     1201 ggtcgaggcc ggccatcgcg atccccccac ccttcccgtt gccgcgattg tgcatctggc
     1261 gcgaggcggt gagcacgtgc cgcccggcga cggggatgct cgacgcgaac cctagcacac
     1321 cgcagccgcc ctcggccgcg tccgcggctc gctccggcgc gggcaggctc ttcatcagct
     1381 cacgccgcga ttcgatgatt cgctcagcga tacgttcggt catgggatag tcctcgtgtc
     1441 ggcaatagct ctcactgcga aactttggtc cagttcgcgt agttcgcccg catctgctcg
     1501 acgtgttccc gaacgtgacg ctcgtacacg tcaagccaat catccatcgt catcacgccg
     1561 ttttcgggat ggagcacggt gttcgaccag accgcttcgg gcagcgactt gatcaaccgg
     1621 tagcttcgga gccgcagcca cttgaacagc tcgacggcct cttcggtggg ctggtcgtga
     1681 taacgcagcg cgtcggccca gcgcatctcg tcgtaggcca tcacgctctc gcccggctcg
     1741 gcgacgaggc ggcggcagcg cacgtagctg ttggcttcgc tgtcggcaat atgaatgacg
     1801 atctcgtgaa tgctccagcg gtcgggcgaa ggcttgaacg tccacatctc cctggggaac
     1861 tgcctcagcg cctccaccag ctcggcatgc gccttcccgt atgattcgat tattgtcagc
     1921 cgatcttcgc gagtcatttg ccctcctctt cactccaggt aaaccaatgc gtcacttcgg
     1981 ccgcgcagct cggcgatcga cttcatgccc agccggtcga gaatacccgc caactgccac
     2041 aaccagcttg tgtacaggtt gacgatcttc ttccagccga tcagcgcgtc gatcttcttg
     2101 gcgtgctcgg gatcggtcgt cgtgatgccg aacgggcagc ccagcccttt ctcgcactgc
     2161 gcgatgcgga tgcagccgat ggcgaccagc tccgccgtgc cgatgatgca gccgtccgcg
     2221 ccgagcgcga tggccttcgc cacgtcgtaa gccgtgcgga tgccgcccga ggccatgagc
     2281 gtgacttcgt ctcgaatgcc ttcctgttcg aggaagcggt gcaccttggg cagggcatac
     2341 tcgatgggca tggcgatgtt cttcttggcg atctccggcg cggcgcccgt cccgccgtac
     2401 gcgccgtcga gctgcaaaat gtgcgcaccg gcgtaatacg atccgacggc gaccatatcc
     2461 acatccgtcg gtgtcgagac tttcacgctg atcagggcgc gcgggttcac ctgcttcagc
     2521 cagtcgacgt gcttcttgtg atcctcgacc gaatacacgc tgtgaaacgg aaacggcgag
     2581 aacagcgacg tgccctgcac ggcttcgcgc aggcgcgcga ctgaatcggt gttcttgttg
     2641 ccgagcaggt gcccgcccag ccctggcttg gcgccctgcg catacttgat ctcgataatc
     2701 ggcgcgcgct gcagcgtgtc ctcgcgcacg ccgaacaacc cggtcgccac ctgcgtgatg
     2761 atgtgatcgg cgtgcggctc cagttcttcg ggatagccgc cctcgcccgt cgaagagaag
     2821 atgccgaggt gctgggccgc tttcgcgcgc gctaacatga ccggcaggcc gacgctgccg
     2881 tacgacatac ccgcgccgta ccaggggatc gggagctcga gcgccggacg ctcgtccgcg
     2941 cggcgattca gcttcaccct gaaatcgaag tgtgccgggt cagccagcgg aacctctcca
     3001 actcccaact ctaactctaa cttgaaggca aggcggtcga agccgccatc gctccgcccg
     3061 acgcggtact cgtactcgcc cgtcggcggc tctcctgttc gagcttgttg atgcgacgac
     3121 agaatcagcg cctgtgtcca gcggaagtcg ccttcggcgc cctccggctc gggcagacgc
     3181 cggtactcct tcactggagc actgccgccg agcatcgatt tgaaggcagc atcttcagcc
     3241 cggtgccaga ttgtccgccc gacctcgccg cgcaggcggc gcatttcgcg catgcccatc
     3301 gcgccgagca cttcgagcag ttgatcgcgc caggcgttca tcaggttgac gacgcgttgc
     3361 cggccccaat ccgcgtcgat ctccttcgcc tcgaccgggc aggcgccctt gtcggcccac
     3421 agcgcacagc cccacgcgat ctgcaggacg aagtcgacgg cgacggcgtc cgcgccgcag
     3481 gcgatcgcct tggcgacgtg gtcggccgcc gcgatgccgc cgctcacgat cagtctcgcc
     3541 tgatcgcgca gtgcctgcga taccagatgc gtatgcacgg cgcgaatcgc gtcgaccagg
     3601 tgccgtcccg atagatcgac gcccatgtcg tcgccgaaca ggtgaatgac cggcacgccg
     3661 tcacgcacga gcgactcaac ttgctgcggc gcgtcgagcg agaacggaac gcggactgcg
     3721 atcacggcat ccggccagcg ctcgcggcaa cgagagatga ggagcgcgcg ggccatctcg
     3781 cgctcctctt cgacttccag gtagcacggc tgccacgcgt caggcacggc gtcgaagttg
     3841 tgggttgaga tgcggggtga tagagattgg agattggagg ttggagattg gaggttggag
     3901 aacaggtcgt cagagagatc ggccgggtcg atgatcgaca gcgtgccgag atcggcggcg
     3961 gcggaagcga gggcgcggat tgcgccgcga ccgggcagcg gaatcggcag cgggttaaac
     4021 acaaccggaa tcggaatggg cagcgagggc ggcagcgccg actgaagccg gccatgctca
     4081 tcaaacgaca gggatgacac accgccgccg atgtccacct gcgtcgagat cgtctcgcga
     4141 ccgtggatgc cgtcgcgagt ggggcgcacg atctcgctca tgtcgagcca gataccctcg
     4201 aaaccgtcgc cgccgaactt gccgccgtag cccgcgccgg agattggaat gcgtccgctc
     4261 tcggcctggt accacgtctg ggtgataatc tcggcggtcc aatactcatc gccgcgctgc
     4321 gacgtgccgg caaagacggg atgaccgtat ttcacgtacg cctcgccttc ggtcagcagg
     4381 aactgtgccg ggtacgcgag cggcatcggc gcggcttcgg tatcgatgtg gtagcggctg
     4441 tgtggtttca acctcaccct ccccctatcc cctccctctc ccgaaacagc tcgggagagg
     4501 gaggggatag gtaaataaaa aaggcacgcc aggcgtgcct tacccattac aatgtgacag
     4561 gcatgcccct ccgttttctt aaacaggatc gcggcgcaac gtgctcgttg agccatcgcc
     4621 tgttaccact gtgtgcgccc tctgtcggcg ggttgcccgc gccggtttac ggccacactt
     4681 tcatcggagg gactgaacgg ggcatggcgg ccccggaagg catccgctga tctctcgatt
     4741 ttccccggcg ttgtgagcga ccgcaaggat cgcccctacg cccgggttaa cctcccctcg
     4801 cgaggaggaa ccttcacctc gtcacccacg catggcgtgg gccttgcgct acgtccctgt
     4861 attcggttgt agtgtttact gcccctactg aggcaaattg tatcacagag gccgtggata
     4921 agtcaatata aggatcgtgt gttgtaacgc atttactacg ggtttcatac gggcacgtcg
     4981 tcgacaggct tatttggccg ccttgaaacg tataccatca gccccccaag cattcccagc
     5041 acatccactg ccaggtcaaa gacactcgca tcccgccccg gcacaaaact ctggtgccac
     5101 tcgtcgctga gggcatatgc ggcgcaccat ccgataacga ggatcttgtt ccatttcgag
     5161 agatggccat ctggcgcggt ggcgcgcgac acgagcagcg ccagcacgac gtagaccgtg
     5221 aagtgaccgg cgatgttgag tagctcttca agccaatctt cttccggtga tggcagcgat
     5281 ggctgcgcag acagaaagaa gatgaacgcc atccaggctg cgacgggaag ccatcgcccc
     5341 agccaaccat tgatcacctt gccgcgtacg atcattccac tctctttcat ccctcatccc
     5401 tcatccttac ccgaccgcgt aggcccgttc gacgctttcg accagtcgat cccacccgaa
     5461 cctctcgcgc acgtccttgg ccgccgcccc gccgaggatg cgggcgcgcc ggcgctcatt
     5521 cagcaattcg acgactgcgc ccgccattgc ctccacatcg ccgctcggcg agaggaagcc
     5581 cgtctcgttg tgacggatgt actccacatt ctgcccgacg gcgtcggcga cgacgggcac
     5641 gcccgccgcc agcaggtcga tcagcttcac ggcgcacttc gtccggttga gcagcgtatc
     5701 gtcgaacgga tagatcgcaa cgccggcgcg tgcaaataca tccggcagct cagccggctc
     5761 gacccagccc agatactcga ttcgatcggt ccaccctcgc tggtgcgctc cgtctatcag
     5821 cgacttttct tcgccgaata aaccctttcc gacgaccaac aagcgcgctt cgggatttaa
     5881 ttccaggaca cggctgaaca ctcccagaac gcgctcgatc ttgaactcaa agaagcggct
     5941 atacaggagg acagtaggag tttggaattt ggatgcagcc tctccaactt ccaacttcca
     6001 acttccaact tccactccat tcgggacgta gaacacgcga gacggagaaa cgcccatcga
     6061 ccagacaatc gactccagcg cgcgactggc gacggtcagc gcgtcgcagt gacgcagacc
     6121 ccactgctcc tgccaggcga atattcgttt ctgcgcccag gagtatcgct cgcggtcgtt
     6181 ccagccgccc gcgccttccc agtcgtcggt gtcgacgatg agccggacgc gcgacaggcc
     6241 cagccctttc agttgccaga gccaccacgc ggcgaggccc gcgtacgcct ttggcttgaa
     6301 gcagtgaacg atgtcaggct ccagcgccag cgcacgcctg accaggcggc gcgtcgtccg
     6361 aacgtggaaa agcagcggca ggtgaggcgg taactcgata ttctcgattc gcacaccgcc
     6421 atcttcgtcg ctgcggccgc tgtcttcagg attcgcccac ggcgggagaa tcatcgtcac
     6481 ggcatggcct cgcgctgcga gcgccctcgc catggggagc gcgcggacag ccatggtgcg
     6541 tttcggtttc agcccgaacg ggccgatcat gacaattcgc atatcgcgta tcgcttatcg
     6601 ctgatggcct gtggcagggt tgtgcggcgg ccaggcgctc gcattatagc agccatcccg
     6661 cccatttcgc cccactctaa ctccatactc taactccccc aattgcctcg ccgtaccgaa
     6721 aagactataa tatcgcccgt gccccacttg cagccaggac gggaggatcg attatgcctt
     6781 acacacacgc gggcaagtac atccgcatcg acttgagcac aggcaagcac acggttgagc
     6841 cgatttcgga cgacgacgtg aagagctacc tgctcggcag cggctacgcg gccaggctat
     6901 tctacgagga aatggacccc tcgatcgacc cactcgaccc gcgcgcgacg ttgtacatct
     6961 tcaatggcct gctgtccggc acgttcgcgc cgaccggctg ccgatcgtcg tggtgcggtc
     7021 ggtcgccgct caccggcatc tggaatgagg ccaacctcgg cgggcatttt ggagcagaac
     7081 tgcgcttcgc cggatacgat ggcatcgtga tcactggcaa agcgccccga ccttcgtatt
     7141 tatacatcaa cggcatcacg aacacgatcg aggtgcgtga cgcgacgcac ctgtggggca
     7201 aagacacgtt tgaggtccac gaccagttgc tcaaagaaac tgacgagaaa gcccgcccgg
     7261 ccgtgatcgg catcgcgggt gagcggctgg tcaagatcgc gggcgtggcg gcaggcggcc
     7321 attcacacac gcgcatggcg gcgcgcggcg gcatgggcgc gctgatggga tcgaaaaacc
     7381 tcaaggcgat cgtcgcacgc ggcaaggaca agccacagta cgccgacgcg aaaggtttcc
     7441 acgcgacggt caaggaaagc aaccattaca tcatggatca ttccgccgcc gaggcgctgc
     7501 acctcgccgg cacggcaggc ggccatccga cgaccgacaa gttcggtgac aacgccatca
     7561 tgaactggcg cggcggcaac tggtacgacg gcacggtcaa gacctccggc aaggcgatcg
     7621 ccgacacgat tttcaccaag cacaccttct gccacgcctg cccgatcggg tgcggcaagg
     7681 cagtcgaggt gaaagacggc agatacgccg gcgtgtgggg cgaaggaccg gagtacgaga
     7741 cactgtgcgg cttcgggtcg aacttgcagt gcgacgatct gaacgcgatc gccgcgatga
     7801 acgacctgtg caaccgctac gccatcgata cgatctcgac gtcgggcgtc ctggcgttcg
     7861 ccttcgaggc ctacgagaag ggcgcaatca cgaaggacga tacgggaggc gtcgagctga
     7921 ggtggggcga tgcggacgcg atcatggcga tgattcataa gattggccgg cgcgaagacg
     7981 tcggcgacac gctggccgag ggcgtgcggg aagcggcgcg gcgcatcggt cagggcagcg
     8041 aggcgtacgc cgtccacgtc aagggtctgg aagtgccgta tcacgatccg cgcggcttcg
     8101 tgagcatggc cgccaactac gcgacggcga gccggggcgc gtgtcacctt gaagcgctga
     8161 cttactggcg cggcgtcggc atcgaatggc cgggctggga ggaaagcgag gcgcaggatt
     8221 ggatcgagcg caagcgtttc gattcaagca tcggcgccca aaccgccatt tcattccaga
     8281 actacatgac gacgtacaac ccgctcggcc tgtgcaagtt catcaccaaa ggttcgttca
     8341 cgccggccca gaccgccgag ctggtcaaca aggcgatggg ctggagctgg acggggcacg
     8401 acgtgctcga cgtgggcgcg cggctgttca acttcaagcg cctgatcaac aaccggctgg
     8461 gcatcgagcg caaggacgac gtgctcccgg tccgactcac caccgagccg cgcccaaccg
     8521 gcacggcggc gggcgtgctg ccggacatgg acgtgatgct gccggaatac tatcggctgc
     8581 ggaagtggga cgagaatggc gtgccgacga aagaggcgat gctgccgtgg gcgaacaacc
     8641 cgtagtgatg gcatccccat gccatcaccc atacccacgg catagggatg ccgtggctac
     8701 ggggtagacc agataattaa catggggcgt ctgacgcaca tttatgaacc gggcatgacg
     8761 tatctgatca ccacgattac gcatcgccgc gagaggctat tcgaagattc gagatatgcc
     8821 cgcacagcgc atgaggacat cgcattctat gcacgcaagt ttggcatgat cagcgtggcg
     8881 catgtgctca tgccggatca cgtgcattgg atcatgcatc cattgccgga gggttttgag
     8941 cgattcacgc gtgaggagca ggcaaagggc aagcgcagca aatatggcca tgcgccggaa
     9001 cgcttctacc tgagcaagat tctcgaagat tacaagcgtc acacggcgca cgcaatcaac
     9061 gaatatcgtg atattcctaa tgcgcaagtg tggcaggaag gttttcgcga tgatgcacta
     9121 cggaccccac aggtcattcg cggggcggtg caatatgtct tgctcaatcc ggtgaaggca
     9181 ggattgacag agcgatctga agattatccg tatttggcat ggaatgcaga ttggttggct
     9241 tgaagctccg gcgtggggag gcaccggcat gggatgcacc ggcatgggga ggcaccggca
     9301 tggggatgcc ggtgctacgg aatgaatgac cgcaggcacg acatccccat gccatgcccg
     9361 tacggcggaa gcgccgatgc cccgcgagag ggcgacagca tgccgtcagt agtggcacac
     9421 gaacaactct ctgcccaagg attaaccgaa tgcccaacac aatgtctgcc gaggaaatgg
     9481 tacgcctgag caaggaatac acactgtggt cgtggtcggc gcaggcggcc gtcaacccga
     9541 tcccgatggt tcgaggccag ggcatctact tctgggacgc cgacggcaag cgctacgtcg
     9601 acttcaactc gcagttgatg tgcgtcaaca tcggccacgg cgaccggcgt gtgatcgagg
     9661 cgatcaaaca gcaggccgag gagct
//
LOCUS       DBNHXB010000407         4618 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2302345, whole genome shotgun sequence.
ACCESSION   DBNHXB010000407 DBNHXB010000000
VERSION     DBNHXB010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2302345"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..605)
                     /locus_tag="AC162G_16295"
     CDS             complement(51..605)
                     /locus_tag="AC162G_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077417.1"
                     /translation="MAKKTTNDKATQKLQALVHKTDLEKPAQKDIAALRQALQQDQAL
                     WRRASDLNHMAHRQLTARFPPAIREALYLRLEAMQQDLGYAEASAIEQLLIEQVATCW
                     LNLQVTQLVYAVATADLTNTASANHWERRLISAQRRYLKAIETLTQVRRRLRPNAVQL
                     NIGTQQLNVVGEVSLRKRRTRSAR"
     gene            1052..2209
                     /locus_tag="AC162G_16300"
     CDS             1052..2209
                     /locus_tag="AC162G_16300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5077418.1"
                     /translation="MRIGLVTGEYPPMQGGVGDFTRELGRALKDLGHDVTVITASSIS
                     GQVAGSPVPVEPIIPKWTQACWRLIADATRRLKLDVLNIQYEPAAYSMQVGVNFFPWW
                     YRRQRERIPVVTTFHDLLVPYLFPKAGALRWKIVELLARRSDAIIVTNEEDRLKLQTP
                     TSKLHLIPIGSNIRPSLPPDFDRNRERAAWGASKSDWLAAYFGFLSMSKGGLTLMRSI
                     GLLVNDGLPVRLLLIGGRTGSSDPTNTQYAAEVDRALASLGLHDRVRHTGYVDPQQVS
                     KAMAAADVIVLPYADGASFRRGSLIAALAHEKAIVTTRPRLAIPELVDGENCVLVPPE
                     DAGALASAVRRVMTEPGLRQRLESGARQIAQKFTWDKIAARSVEVFRGVLL"
     gene            2206..4536
                     /locus_tag="AC162G_16305"
     CDS             2206..4536
                     /locus_tag="AC162G_16305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR5077419.1"
                     /translation="MSRWIVRLLALALLWIMLARMLHASSALSPIVDEQSHISRGLAV
                     LRTGDLRLRIGHPIGLNVWQALPLALDSTVRLPLEHASWANAAWDRFGEQFLWRVNEN
                     PDGIVFRSRVMVMLLTLVLGAIVFRWAEELYGEPSGLLALTLYTLDPNILAHGMLATT
                     DVGVTLFIVAAVFALWRAARILSGAQRSRRTDRRWFVAAGVFAGLALVSKYSALVLVP
                     IFVALALLLIGVARASGALVSRRFISKRGAVSGLLVLVTALLVVLIVYALRVDLYLDE
                     FGFLLANTGTHPSFLLGQRSIEGWWYYFPVTFLLKTPLSTLLLIGLAAVLSARHRSWR
                     DELFLLVPALLYFLFGALSGFNVGYRHLFPTLLFFLVFASKSGQAWQPSRIILRLRSG
                     QACHASRLRGFISTLVIGLLMLWLALSSFTAHPNYIAYFNELAPTPHYDVLVDSNLDW
                     GQGLKQLRSYLDEHGIERVRLGYFGTADPVAYGIAYDPLPRWPPPRSVDFVPANPAPG
                     VYAISASNLQGVLLDEPSAFDWFRRRDPDAVVGGSILIYHVSADPDPPQAVGLCREPW
                     TPVDDEKVNALFGREGLRVVRFDCVDAWWWPDEPTWYVVPPDVLDPPVKVVTYVRRRQ
                     DDTIVFRALRGPDVAPAPDHPLDTPARFEGGLTLLGFNGSGGATTGNALVVDSVWRVD
                     APPNPPVSVFAHLLDPDGAFVLAADGLGVPVESMRVGDVIAQRHQFDVSPDAATGEYT
                     IEFGFYRLDTLERYPVIVAGRMAGREIRVGPFAIRR"
BASE COUNT          711 a         1488 c         1388 g         1031 t
ORIGIN      
        1 ggccacgagc acgcagtgcg ccaccagaat ttgcaccacc aggatactgc tcagcgcgca
       61 ctgcgagtcc tccttttcct aagactgacc tccccgacca cgttcaactg ctgcgtgccg
      121 atattcaact gcaccgcgtt tggcctcagc cgtcgccgca cctgcgttag cgtttcaatg
      181 gccttcagat agcgccgttg ggctgagatc aggcgccgct cccaatgatt ggcgcttgcg
      241 gtgttcgtga gatcggcagt cgccaccgcg tatactagct gtgtgacttg caggttcagc
      301 cagcacgtcg caacttgttc aatcagtagt tgctcaatcg ccgacgcttc ggcataaccc
      361 aggtcttgct gcatggcctc cagccggaga tagagcgcct cacgtatcgc cggtgggaac
      421 ctggccgtca gttgccggtg agccatatga tttaggtcac tcgcccggcg ccagagtgcc
      481 tgatcctgtt gtagcgcctg acgcagcgcc gctatgtcct tctgcgccgg cttctcaaga
      541 tcggtcttgt gaaccagcgc ctggagcttc tgggtagctt tgtcgttcgt cgtcttctta
      601 gccattccgt ttctttctcc attgtcctcg gtgggtatga taaccttcgg caatccacgt
      661 tgctcgcacc agggcttgag tcaatgtctg tatgtcgtcc aggatcatct ccacgctctc
      721 gatttcggcc catgctttac gctcattggc gcgtcgctgc cgtaaccacc cgtcactcgc
      781 cgccgccagc tccgctgcgc gccccgtgcc gatatactcc gacaccacgc gcccaccctc
      841 tcgtcgtttg cgataatagt agtattgact gcggcgtttc tcccaactca tgttgtgcta
      901 cattaccttt ctatccccgc tttgatgatc tcacataagt gctgcgttgt caaatgaacc
      961 gatcaagcag atcgggcgcg ttgcatgagt ttgaccttac ttgtccatcc ggctatactt
     1021 tccattcggg ggctctcatt tcgatgcaca catgcgcatt ggattagtca ccggcgaata
     1081 cccgcccatg cagggcggcg tgggcgattt cacccgcgag ctggggcgcg cgctcaagga
     1141 tctcgggcac gatgttacgg taatcaccgc gtcgagcatc tccggccagg ttgccggttc
     1201 gccggttccc gtcgaaccca tcatccccaa atggacacag gcctgctggc gcctgatcgc
     1261 cgatgcgacc cggcggctga agctcgacgt gttgaacatc cagtacgagc cggctgcgta
     1321 ctcgatgcaa gtcggcgtca acttctttcc gtggtggtat cgacgccagc gagagcgcat
     1381 ccccgtcgtg acgacctttc acgatctgct tgttccgtac ctcttcccca aggccggcgc
     1441 gctacgctgg aagatcgtcg agctgcttgc ccgccgcagc gatgccatca tcgtcaccaa
     1501 cgaagaagat cgtctcaaac tccaaactcc aacctccaaa ctccacctca taccgattgg
     1561 ctcaaacatc cggccctccc ttccaccgga cttcgatcgc aaccgtgagc gcgcggcgtg
     1621 gggcgccagt aagagtgact ggctggcggc atacttcggt tttctgagta tgagcaaggg
     1681 cgggctgaca ctgatgcgca gcatcggcct cctcgtcaac gatggcctgc ccgttcgact
     1741 gttgctcatt ggcgggcgaa ccgggtcgag cgacccgacg aacacccagt atgccgccga
     1801 ggttgatcgc gccctggcgt cgctcggcct gcacgatcgt gtgcgccata ccggctacgt
     1861 ggatccgcag caggtgtcga aggcgatggc tgcggcggac gtcatcgttc tgccatacgc
     1921 cgacggcgcg tcatttcggc gcgggagtct gatagctgcg ctggcgcacg agaaagcgat
     1981 tgtcacgacg cgaccgcgcc tggcgattcc cgaactcgtc gacggcgaga actgtgtgct
     2041 tgtcccgccg gaggatgcgg gggcgcttgc gtcggccgtg cgccgggtga tgaccgaacc
     2101 gggcctgcgc cagcgactgg aatccggcgc gcgccaaatc gcgcagaagt tcacgtggga
     2161 caagatcgcc gcgcgctccg tcgaggtgtt tcgcggagtc ctgttgtgag ccgctggatc
     2221 gtgcgacttc tggcgctcgc gctgttgtgg attatgttgg cccgcatgct ccacgcatcc
     2281 tcggcgctgt ccccgatcgt agacgagcag agccatatct cgcgaggtct ggcggtcctc
     2341 cgcaccggcg acctgcgatt gcgcatcggg catcccattg ggttgaatgt gtggcaagcc
     2401 ctgccgctgg ccctggattc gacggtgcgg ctgccgctcg agcacgcgtc atgggcaaac
     2461 gccgcctggg atcgctttgg agagcagttc ctgtggcgcg tgaacgagaa cccggatgga
     2521 atcgtcttcc gttcgcgtgt gatggtgatg ctgttgacgc tcgtcctggg cgcaatcgtg
     2581 ttccggtggg cagaggaact gtacggcgag cctagcgggc tgctggcgct tacgctgtac
     2641 acgctcgatc cgaatatcct ggcacacggc atgctggcga cgaccgacgt cggtgtgacg
     2701 ctattcatcg ttgccgccgt gttcgcgctg tggcgcgccg cacgcatcct gagcggagcg
     2761 cagcgaagtc gaaggaccga tcggcggtgg ttcgtggcgg ccggtgtgtt tgcgggactc
     2821 gccctcgtct ccaaatattc agcgctggtt ctcgttccga tcttcgttgc cctcgcattg
     2881 ctcttgatcg gagtggcgcg cgcgagcggg gcgctcgttt ctcgccgctt catctcgaag
     2941 cgtggcgcgg tcagcgggct gctcgtcctt gtcactgcgc tgctcgtcgt tctgatcgtg
     3001 tacgcgttgc gcgtcgacct gtacctggat gagttcgggt ttctcctcgc caatacggga
     3061 acacatccct cgtttctgct tggccagcgc tcgatcgagg gctggtggta ttactttccg
     3121 gtcacgttcc ttctcaagac gcccctgtcg actctgctgc tgatcggcct cgccgccgtg
     3181 ctcagcgctc gccatcggag ctggcgcgat gagttgttcc tgctcgtccc ggcgctcttg
     3241 tactttctct tcggcgcgct cagcggcttc aacgtcggct atcgccacct gttcccgacg
     3301 ctcttgtttt tcctcgtgtt cgcctccaag tccgggcagg cgtggcagcc ttcacgtatc
     3361 atccttcgac tccgctcagg acaggcttgt cacgcatcac ggctgcgcgg cttcatatcc
     3421 acgctcgtga ttgggctact gatgctgtgg ctcgccctct cgtccttcac agcccacccg
     3481 aactacatcg cgtacttcaa cgagctggct cccacgccac actacgatgt cctcgtggat
     3541 tctaatctcg actggggaca gggcctcaag caattgcgct cgtatcttga cgagcacggg
     3601 atcgagcgcg tgcgcctggg ctactttggc acggccgatc cggtggcgta cggaatcgca
     3661 tacgatccgt taccgcgttg gccgcctccc aggtccgtcg atttcgtccc ggcgaacccc
     3721 gctcctggcg tgtacgccat cagcgcgagc aatttgcagg gtgtcctgct cgacgaaccg
     3781 agtgccttcg actggttccg gcggcgtgac cctgatgccg ttgtcggcgg atcgatcctg
     3841 atttatcatg tctcggccga ccccgaccct ccgcaagccg tcggcctgtg ccgcgagcca
     3901 tggacgccgg ttgacgacga gaaggtgaac gcactgtttg ggcgcgaggg gctgcgcgtc
     3961 gttcgcttcg actgcgtcga tgcgtggtgg tggccggacg agccaacctg gtacgtggtg
     4021 ccgcccgacg tgctagaccc gccggtcaag gtcgtcacgt atgtgcgtcg ccggcaagat
     4081 gacaccatcg tctttcgggc gcttcgaggt ccggacgtgg ctccggctcc ggatcatcca
     4141 ctggacacgc cggcccgctt cgaaggcgga ctgacgctgc tcggcttcaa cgggtcgggt
     4201 ggagcgacga caggcaatgc tctcgttgtg gactcggtgt ggcgggtcga cgcgccgccg
     4261 aacccacccg tgtcggtctt cgctcacctg ctcgatccgg atggcgcgtt cgtcctcgca
     4321 gccgatggat tgggtgtgcc ggtggagtcg atgcgtgtgg gcgatgtgat cgcgcagcgt
     4381 caccagttcg acgtgtcgcc cgatgccgcg accggcgagt acaccatcga gttcggtttc
     4441 taccgactcg atacgttgga gcgctacccg gtgatcgtcg ctgggcggat ggccgggagg
     4501 gagattcgcg tcggtccgtt tgccatccgc cggtagcgac cgtgcactcc ggcggcatct
     4561 cgctgcacac atcgcatgac gctccttcat cgttttgccg acatccgaat catcgtca
//
LOCUS       DBNHXB010000408         2727 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_959978, whole genome shotgun sequence.
ACCESSION   DBNHXB010000408 DBNHXB010000000
VERSION     DBNHXB010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_959978"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="AC162G_16310"
     CDS             <1..335
                     /locus_tag="AC162G_16310"
                     /inference="COORDINATES: protein motif:HMM:NF024645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR5077420.1"
                     /translation="AAGVLRQIDALFEAMPGARVETREDEIERAVAARRQSKRAATGS
                     TGADLEAAIEARRKRKPAPATMDDEIEAAVAARRQALSCPACGKPIQAGDAFCPKCGA
                     PLGTQVAR"
     gene            464..2287
                     /locus_tag="AC162G_16315"
     CDS             464..2287
                     /locus_tag="AC162G_16315"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5077421.1"
                     /translation="MLAVVVSGCALSAPPPPRGSAPILTPLPPPEAAFELPARRPSVA
                     QGAAIYAEKCAACHGPSGKGDGERAAQIQSTFGVAPADLTSEQIVRTSTVAEWFALIT
                     AGRLDENGQPRGMPGFSGSLSADDRWDVIAYVWSLGSPDSTLAEGEAIYAERCVQCHG
                     ETGRGDGPQADGTVPDLSDLAVYRDTAFGAWDNALQSTHVPSFSGKLNGLERSAVIDY
                     VRSFTYDSAPTVAEPEPAATPALDTAPAGPDEPGLTVNGTIANGTGGVDTPANLEVTL
                     FHFPGGTGDTVITRTVTTDEAGAFTASYADIAPGDIVMASLTYADVTYRDGLEYDGTT
                     STADLQFRVFEPTSETGAIRLETLHVIAMPGSDAIDVNEIYVISNLGDRVLVNTTGEP
                     ALRFDLPDEASAVQLLDGAVQGAFAASPDGFDYYEAVSPGEDTARLVIAYQLPLGAAD
                     AVFDRTLSYPAQSVNVLVASQDWQGSSSQLLDRGPQVIQGQTYQQFSGGPIAAGQTLA
                     LRLTPPRPAFDPKLAAGIAILLVGVAVVGYGVWRTRAQQEQPSRGARPAPKARPAGQD
                     ALIDQIAALDDAFEAGEIRESEYRKQREALKARALRLMREE"
     gene            2368..>2727
                     /locus_tag="AC162G_16320"
     CDS             2368..>2727
                     /locus_tag="AC162G_16320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5077422.1"
                     /translation="MIQVSGLVKQFGPFVALREVDFRVEPGEFVSLMGPNGAGKTTLL
                     RILSTLSRPTRGTVTIAGHTLPKGADKARRHIGFLSHQPLLYGELSAEENLRFFAHLY
                     DLPQNGGRIDALLEQVGL"
BASE COUNT          477 a          921 c          916 g          413 t
ORIGIN      
        1 aggcagccgg cgtgctgcgg cagatcgacg cgctgtttga agcgatgccc ggcgcgaggg
       61 tcgagactcg ggaagatgag atcgagcgcg ccgtcgcggc gcgccgccag agcaaacggg
      121 cagccacagg ctcgaccggg gctgacctgg aagccgcgat cgaggcgcgg cgcaagcgca
      181 agcctgcgcc ggcgacaatg gacgatgaaa tcgaggcggc ggtcgccgcg cgccgccagg
      241 cgctcagttg ccccgcctgc ggcaagccca tccaggccgg cgacgcgttc tgccccaagt
      301 gcggtgcgcc gctcggcacc caggtcgccc gatgagactc cttcaccccg cagcgcctcg
      361 ccaagcccgg cctttcttaa ccacgaagac acgaagacac gaagacaact tagtgccttt
      421 gtgtcttcgt ggtaagacgc tgccgatgat agcgatgtgt ctgatgttgg cagtcgttgt
      481 gtctggctgc gccctgtcgg cgccgccgcc gccgcgcggg agcgcgccga tcctcacacc
      541 gctgccaccg cccgaggccg cgtttgagct gccggcgcgc aggccttctg tcgcccaggg
      601 cgcggcgatc tacgccgaga agtgcgccgc gtgtcacggg ccgagtggca aaggagacgg
      661 cgagcgcgcg gcgcaaatcc agtccacctt cggagtcgcg ccagcggatc tgacatccga
      721 gcagatcgtc cgcacgtcga cggtagcgga gtggtttgcc cttatcacgg ccggccggct
      781 cgacgaaaac ggccagccgc gtgggatgcc gggcttcagc ggatcgctgt cggcggacga
      841 tcgatgggac gtgatcgcct acgtgtggtc gctgggatca cccgattcga cgctggccga
      901 aggcgaggcg atctacgccg agcgctgcgt gcaatgccac ggcgaaaccg gcagagggga
      961 cggcccgcag gccgacggga ccgtgcccga tctgtccgat cttgccgtgt atcgcgacac
     1021 ggccttcggc gcatgggaca acgccctgca aagcacgcac gtgccatcgt tcagcggaaa
     1081 gctgaacggc ctggagcgca gcgcagtgat cgactacgtt cgcagcttca cgtatgacag
     1141 cgcgcccacc gtcgccgagc cggaaccggc cgcgacgcct gcgctcgata ccgcccccgc
     1201 cggccccgac gaaccgggcc tgactgtcaa cggaacgatc gccaacggca ccggcggcgt
     1261 cgacacgccg gccaaccttg aagtgacgtt gttccatttc cccggcggca caggcgacac
     1321 ggtcatcact cgcaccgtca ccacggacga ggcgggcgcg ttcacggcgt cttacgcgga
     1381 catcgcgccc ggcgacatcg tcatggccag cctcacctac gccgacgtga cttatcgcga
     1441 cggccttgag tacgacggga cgacgtcgac ggccgatttg cagtttcggg tgttcgagcc
     1501 gacttcggaa acgggcgcca tccggctcga gacgctgcac gtgatcgcca tgcccggaag
     1561 cgatgcaatc gacgtcaacg agatttacgt gatctccaat ctcggcgacc gcgtgctggt
     1621 gaacacgacc ggtgagccgg cgctccgctt cgatctgccg gacgaggcga gcgccgtgca
     1681 gttactggac ggcgcggtgc agggcgcgtt cgccgcatcg ccggacgggt tcgattacta
     1741 cgaggcggtc tcgcccggcg aagacaccgc ccggctggtc atcgcctacc agttacctct
     1801 cggcgcggcc gatgcggtct tcgatcggac gctgagctac ccggcacagt cggtgaacgt
     1861 attggtcgcc tcgcaagact ggcaaggctc gagcagccag ttgctcgatc gaggccccca
     1921 ggtcatccag ggccagacct atcagcaatt ttccggcggg ccgattgccg cggggcagac
     1981 gctggccttg cgtctgacgc cgccgcgccc cgctttcgat ccgaagctgg cagccggtat
     2041 tgcgattctg ctcgtcggcg tggccgtcgt aggctacggt gtgtggcgca cgcgggcaca
     2101 acaggaacag ccgagccggg gcgctcgccc ggcgcccaaa gccaggcctg ccggccagga
     2161 tgcgctgatc gaccagatcg ccgcgctcga cgatgccttc gaggccggcg agatccggga
     2221 atccgagtat cgcaagcagc gcgaagcact gaaggcaaga gccttgcgcc tgatgcgaga
     2281 agagtagagc cagtcgacga gtcagcgagt cagcggaacg ggtggcccga acgctgactc
     2341 gctgattcgc ggattcacga gacacccatg attcaagtct ccggactcgt caagcaattc
     2401 ggcccgttcg tcgcgctgcg cgaggtggat ttccgggtcg agccgggcga gttcgtctcc
     2461 ttgatgggac cgaacggcgc gggcaagacg acgctgctgc gcatcctgtc gacgctgtca
     2521 cggccgacgc gcggaacggt gaccattgcc ggacacacgc tgcccaaggg cgcagacaaa
     2581 gcgcgccggc atatcgggtt cctgtcccac cagcccctgc tgtacggcga gctgagcgcc
     2641 gaagagaacc tgcgcttctt cgcccacctg tacgacctgc cgcagaacgg cgggcgcatc
     2701 gacgcgctgc tcgaacaggt cggcctg
//
LOCUS       DBNHXB010000409         4831 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2470843, whole genome shotgun sequence.
ACCESSION   DBNHXB010000409 DBNHXB010000000
VERSION     DBNHXB010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2470843"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..1381
                     /locus_tag="AC162G_16325"
     CDS             302..1381
                     /locus_tag="AC162G_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077423.1"
                     /translation="MIAYIIRRLLMMPLLLFGVSLLVFGMTSLLSPQERLALYLRELP
                     RNEEQAETLIRKYGLDEPFLVQYANWLFGRPVTDPATGETVTSGGVLRGDFGYSRTGS
                     DAIVNIIKRRFPVTLELTLWSILPIVGLGVAMGVAAAVRHNKMGDQALRVFSIVGWSM
                     PSFVFGLLMLMIFYARLQWFPPDNLSDWASEVVSDATMFTRYTGMNTLDSLLNGRPDI
                     FLDSLRHMVLPILTLAYIQWALLVRITRASMLESLRQDYVTTARSKGLDERAVIHRHA
                     RPNAMVPVVTVGGFVIVGLLNGVVITETIFNYPGIGSVAAAAAAQLDVLTVLGFTLFN
                     GVILLAANLVVDVLYAVVDPRVRLS"
     gene            1431..2456
                     /locus_tag="AC162G_16330"
     CDS             1431..2456
                     /locus_tag="AC162G_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077424.1"
                     /translation="MAVAVPTRSADIGSAVTRKPPSLVRQLLANRLAVVGFALVGFFA
                     FVAIAGPVLAPPPENARDPMKIPRDGFGTVPLPPGSPWVRNAPPLPFWWKPLTGLDHW
                     THVFGVASGGWDIYYGVVWGTRTAFIGGIIITFVTVLIGVIVGTLSAYYGGLVDTVLQ
                     RLTEVFLAFPFLLAALTLASVLQPLLGGKTGIGGSGLITAGIAVVMFGWMGFARLIRG
                     DILSVRERDYVTAARAVGVRDRRIMTRHIIPNAIFPTLVLASLNIGTNAITFAALSFL
                     GLGAEQGFADWGQLLAFSRNWITTLDQYWYMVFFPAVALVLFVLGWNLVGDAVRDVLD
                     PRTRQTR"
     gene            2653..3648
                     /locus_tag="AC162G_16335"
     CDS             2653..3648
                     /locus_tag="AC162G_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006456394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077425.1"
                     /translation="MPLLLQVNDLATRFHTPEGTVHAVNGVSFELKEGETLGVVGESG
                     CGKSVTVLSLLRLIPQPPGEIVRGEALFAGRDLLQLTTDQMRQVRGAQIAMVFQDPMT
                     SLNPVMPIGRQISESLIEHLGMNQQQARARVVELLRLVGIPDAEKRLDDYPHQFSGGM
                     RQRVMIAIALACNPQVLIADEPTTALDVTIQAQIIELVKRLRAELGMAVIWITHDLGI
                     VAGIAQRVQVMYAGYIVEKADVKELYANPRHPYTLGLMGAVPRVDGRHGQRLANIQGR
                     PPDLLTEPRACPFAPRCPFSIEQCWQENPALREVGPRHEIACWVDVTKEDVGRGF"
     gene            3638..4651
                     /locus_tag="AC162G_16340"
     CDS             3638..4651
                     /locus_tag="AC162G_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077426.1"
                     /translation="MASNDRVLVRVANLKKHFPIRRGVFQRTVGWVKAVDGVSFEIKR
                     GETLGLVGESGCGKTTVGRTILRLYPPTGGHIFFDSVELTAMKGETLRRLRPRMQMIF
                     QDPYASLDPRSTVGELIAEPLLIHGLADQQGRRERVKQLMELVGLNPAYLTRYPHEFS
                     GGQRQRIGVARALSLNPEFIVCDEPIAALDVSVQAQVVNLLEELQEKLELTYLFVAHD
                     LSMVRHIADRVLVMYLGKMMEMGERDELYDNPLHPYTQALLSAVPVPDPVAEEARRRI
                     ILQGDVPSPANPPKGCVFHTRCPLAIDVCRQVEPEWREFTPGHFVACHRAEEIARKEV
                     VVR"
BASE COUNT          802 a         1544 c         1574 g          911 t
ORIGIN      
        1 gatgattttg cagcgcgagg cgacgctcga cggcgtgacc ttcctgcagc gcatggtctg
       61 gcacaacatc gcgtcgaacc gcctggactg gaactgggaa cgcagcgacg atcgaggtcg
      121 gacatggaac gtattgtggc acattcacta cgagcgagtg acggactagt attccgccac
      181 atgccaaatg atgggtagcc tagccccttg tgggcgtctg acgggcacga ggccctacgc
      241 tacgagcgag tgacagacca gagcggataa gcaggttcgg cctgcgccgc aggagacgac
      301 catgattgct tacatcattc gacgcctact gatgatgccc ctgttgctgt tcggcgtgtc
      361 gctgctcgtc ttcggcatga ccagcctgct ctcgcctcaa gagcggctgg cgctctacct
      421 gcgcgaattg ccgcgcaacg aagagcaggc cgagacgctc attcgcaaat acggcctgga
      481 cgagccgttc ctcgttcagt acgccaactg gctgttcggg cgcccggtca ccgatccggc
      541 gaccggcgag accgtgactt ccggaggcgt cttgcgcggc gacttcggct actcgcgcac
      601 gggatccgac gcgatcgtga acatcatcaa gcggcggttc ccggtcacgc tggagttgac
      661 cctctggagc atcttgccca tcgtcggcct gggcgtcgcg atgggcgtgg cggccgccgt
      721 ccgccacaac aagatgggcg atcaggcgct gcgcgtcttc agcatcgtcg gctggtcgat
      781 gccgtcgttc gtattcggcc tgctgatgct gatgatcttc tacgcgcgcc tacagtggtt
      841 cccgccggac aacctgtcgg actgggccag cgaggtggtg agcgatgcga cgatgttcac
      901 gcgctacacg gggatgaaca cactcgactc gttgctgaac gggcggccgg acattttcct
      961 cgactcgctg cgccacatgg tgctgccgat cctgacgctc gcctacattc agtgggcgct
     1021 gttggtgcgc atcacgcgcg cgtcgatgct ggagtcactc cggcaggatt acgtgacgac
     1081 ggcccgatcg aaagggcttg acgagcgcgc cgtcatccac cggcatgccc ggccgaacgc
     1141 catggtcccc gtcgtgaccg tcggcgggtt cgtcatcgtc ggcctgctga acggcgtggt
     1201 gatcaccgag acgatcttca actacccggg catcggatcg gtggcggccg ccgccgccgc
     1261 gcagctcgac gtgctcacgg tgttgggatt caccttgttc aacggggtga tcctgctcgc
     1321 cgccaatctc gtcgtcgacg tgctatatgc cgtcgtcgat ccgcgtgtgc ggttgagtta
     1381 gagttggggg ttagagttag agttagaggt tggaggttgg aggtaggaga atggcggttg
     1441 ctgtccctac acgatctgcc gacatcggaa gcgccgtaac tcggaagccg ccgagcctgg
     1501 tcaggcagtt gctcgcgaat cggctggccg tggtcggctt tgccttggtg gggttcttcg
     1561 ccttcgtggc gatcgccggg ccggtgctgg cgccaccgcc cgagaacgcg cgcgacccta
     1621 tgaagatccc acgtgacggc ttcggcacgg tgcctctgcc gcccggctcg ccctgggtcc
     1681 gcaacgcgcc gccgctcccg ttctggtgga agccgctgac cgggctggat cactggacgc
     1741 acgtgttcgg cgtggcgagc ggcgggtggg acatttacta cggcgtcgtg tgggggacgc
     1801 gcacggcgtt cataggcggc atcatcatca cgtttgtcac cgtcctgatt ggggtgatcg
     1861 tcggtacgct ctccgcgtat tacggtggcc tggtcgacac ggtcttgcag cgcctgacgg
     1921 aggtgtttct cgcgtttcca ttcttgctcg cggcgctcac gctggcgtcg gtcctccagc
     1981 ctctcctcgg cgggaagacg ggcatcggcg gcagcggcct gatcacggca ggcatcgccg
     2041 tcgtcatgtt cggatggatg ggcttcgcgc gcctgattcg cggcgatatc ctgtccgtgc
     2101 gcgagcgtga ttacgtgacg gcggcgcggg cggtcggcgt gcgcgaccgg cgcatcatga
     2161 cgcgccacat catcccgaac gccatcttcc cgacgctggt tctggcatcc ctcaacatcg
     2221 gcacgaatgc gatcaccttc gccgcgctgt ccttcctcgg tttgggcgcc gagcaggggt
     2281 tcgccgactg gggtcaactg ctggcctttt cgcgcaactg gatcacgacg ctcgaccaat
     2341 actggtacat ggtgttcttc cccgccgtgg cgctggtgct gttcgtgctc ggctggaacc
     2401 tggtgggaga cgcggtgcgc gacgtcctcg atccgcgcac gcgccagacg cgataagcct
     2461 gccgttaaac ccatgttggg gcgacagttg tactgtcgcc tggcgtccgc agtgcaactg
     2521 cggacgaacg gacgcctgca gttgaacctt ctcacttgcg ttccctaacc cttattgtat
     2581 aattaaatag ccaatcgctt ctttttttcc tcgccattga accttttacg ccaaatgggg
     2641 agtatttaac atatgccgct tcttcttcag gtaaacgatc tcgcaacccg cttccacacc
     2701 ccggaaggga ccgtgcatgc cgtgaacggc gtgtctttcg agctgaagga aggcgagacg
     2761 ctgggcgtcg tcggcgagag cggctgcggc aagagcgtca ccgtgctctc gctgctgcgt
     2821 ttgatccccc agccgccggg cgagatcgtc cgaggcgaag cgctgttcgc cggacgcgat
     2881 ctgctgcagt tgacgacaga ccagatgcgc caggtgcgcg gcgcgcagat cgcgatggtt
     2941 ttccaggacc cgatgacctc gctcaatccc gtgatgccca tcggccgcca gatctcggag
     3001 tccctgatcg agcacctggg catgaaccag cagcaggcgc gcgcgcgcgt ggtcgagctg
     3061 ctgcggctcg tcggcatccc ggacgccgag aagcggctgg acgattaccc gcaccagttc
     3121 tcgggcggca tgcgccagcg cgtgatgatc gccatcgccc tggcctgcaa tccacaggtg
     3181 ctgatcgccg acgagccgac gacggcgctg gacgtgacga tccaggcgca gatcatcgag
     3241 ctggtcaagc ggctgcgcgc cgagctgggc atggccgtga tctggatcac gcacgacctg
     3301 gggatcgtgg ccggcatcgc ccagcgggtg caggtgatgt atgcggggta catcgtcgag
     3361 aaggccgacg tcaaggagct gtacgccaac ccgcgccatc cgtacacact cggcctgatg
     3421 ggcgccgtgc cgcgcgtcga cggccgccac ggccaacggc tagccaacat ccaggggcgg
     3481 ccaccggacc tgctgaccga gcctcgcgcc tgtcccttcg ccccacgctg tcctttctcc
     3541 atcgagcaat gctggcaaga aaacccggcg ctccgcgaag taggcccccg ccacgagatt
     3601 gcctgctggg tggacgtgac gaaggaggac gtgggtcgtg gcttctaacg accgcgtgct
     3661 ggtgcgcgta gcgaacctga agaagcattt cccgatccgg cgcggcgtct tccagcgcac
     3721 cgtcggctgg gtgaaggccg tcgacggcgt ctcgttcgag atcaagcgcg gcgaaacgct
     3781 aggcctggtc ggcgagagcg gctgcggcaa gacgacggtg ggccgcacga tcttgcggct
     3841 gtacccgccg acgggcggcc acatcttctt cgattcggtc gagctgacgg cgatgaaggg
     3901 cgaaacgttg cgacggctgc ggccccggat gcagatgatc ttccaggacc cgtacgcctc
     3961 gctcgacccg cgctcgacgg tcggcgagct gatcgccgag ccgctgctca tccacggcct
     4021 ggccgaccag caggggcggc gcgagcgggt caagcagttg atggagctgg tcggcctcaa
     4081 cccggcctac ctgacgcgct atccgcacga gttttcaggc ggccagcgcc agcgcatcgg
     4141 cgtcgcgcgc gccctgtcgc tcaaccccga gttcatcgtc tgcgacgagc cgattgccgc
     4201 gctcgacgtc tcggtccagg cgcaggtcgt caacctactc gaggagctgc aggaaaaact
     4261 ggagctgacc tatctattcg tcgcccacga cctgagcatg gtgcggcaca tcgccgatcg
     4321 cgtcctggtg atgtacctgg gcaaaatgat ggagatgggc gagcgcgacg agctgtacga
     4381 caatccgctt catccgtaca cgcaggcgct gctctcggcc gtgccggtgc ccgatcccgt
     4441 cgccgaggaa gcccgccggc gcatcatctt gcagggcgac gtccccagcc cggccaaccc
     4501 gccgaagggc tgcgtgttcc acacgcgctg cccgctggcg atcgatgtgt gtcgccaggt
     4561 cgagccggag tggcgcgagt tcacgcccgg ccatttcgtc gcctgccatc gcgccgaaga
     4621 gatcgcacga aaggaggtgg tcgttcgcta agaacccatc cgaaaactcc gtagctggtg
     4681 catcccaatg cgccagcaag cggcatgggg atgccgccct acggtccgca aagtcgtatc
     4741 cgcaacatgc aaccatactc tggaggagta agaagacatg tctaagagaa tgagcattct
     4801 cgtgagcctg ctggtgctga cggccatcgt g
//
LOCUS       DBNHXB010000410         6393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_960771, whole genome shotgun sequence.
ACCESSION   DBNHXB010000410 DBNHXB010000000
VERSION     DBNHXB010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_960771"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..759
                     /locus_tag="AC162G_16345"
     CDS             73..759
                     /locus_tag="AC162G_16345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR5077427.1"
                     /translation="MVRSLWLHDGVNQIGIRSNVRVIRAKSALIAGEVVVEDKVAPSL
                     TRLNKAQLLSYLKVTGKQVGLLLNFGGPMPEFQRLFFAPREPKIVDAYTERAVSNVER
                     PDLVAPEMVYKIIGGLFEVHATLGPGFVHRIYTNACHHELKLRDLPARLQKTIQVFYR
                     DISLGELKFGHLCVGDAVIVFPTAIQSIADLKLDALREWLRVQEIPLGILANFNDTEL
                     KPIFLKPRSD"
     gene            832..1299
                     /gene="dtd"
                     /locus_tag="AC162G_16350"
     CDS             832..1299
                     /gene="dtd"
                     /locus_tag="AC162G_16350"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR5077428.1"
                     /translation="MRLVIQRVKQASVFVDGKIVGQCGHGLCILAGVRVGDTEAEAKW
                     LAQKVAGLRIFEDDEGKMNRSAIDVGGSALVVSQFTLYADARKGRRPSFVDAELPDKA
                     ATLVMKFVEYLRAEGVPTETGVFGAMMLVEIHNDGPVTIILEREAGQTKVIGD"
     gene            1448..2815
                     /locus_tag="AC162G_16355"
     CDS             1448..2815
                     /locus_tag="AC162G_16355"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR5077429.1"
                     /translation="MLKRSVACVVVLVVTACSTTAPTTEPAATSEPTATPKPTLTSTA
                     AQAASLTPTATPQPAVSPTDLPTDTPTAKPTIQPADTAASQPAARPTNPPAAPPAIGD
                     ATPKPPPGSGSCGNYPCDDDIAGWVNRIQAPSAFKVSHVARAPNGEHITSITHGPDGR
                     LFASTMEGNVYVFDAAGNPSLYTSGFNLPIGLAFRPGTSDLYVASRTFAPDAVPNEGK
                     VTIVRPDGSKQDIVTGLPCCYTAVEHQPNSIAFGPDGKVYLSIGARADHGEEPGKPDV
                     PATLTPYEAGILRFNLDGSGLERYAVGLRNPYDLAFDASGRLFATDNGPDYGPPERLH
                     AIVQGGHYGYPYYDCDICQKAPPDLAITPPIAQFIAHGAITGITAYTANAFPSNYYND
                     LFVVLWSAFPGAQKVVRVSTFPTAVSDFALGFAAPIDVTQSPDGALLVADWATGHIFK
                     IAYSP"
     gene            complement(3051..4298)
                     /locus_tag="AC162G_16360"
     CDS             complement(3051..4298)
                     /locus_tag="AC162G_16360"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5077430.1"
                     /translation="MSALSIRFQRIGQPAAFLVGVIFTLAVAVIAIELVMHPPRADLE
                     QLALGLSLTSLGAFAAGYVLYRLGWWRWPRRILHTMIFGSILQAALLFISVWITARNM
                     FLNYHDLALAGVLLLFGAGIAVSFGAFVSASLTRGLRRVEQAAQRLAGGDFSARAEVD
                     GRDEVSRLAITFNHMAHQLEAAARKQRELDTLRRDLIAWTSHDLRTPLTSIRVVVEAL
                     ADSMVDDPETIQRYLNSIRSDVASLNSLINDLFELAQLDAGGLKFDKVSVSLSDLISD
                     VLERMRALAERRQVRLAGQVSPQVDPVTIDPQKIERVLTNLISNALRHTPEGGHVTID
                     ARRHDGKVRVSVSDTGEGISAEDLPRVFERFYRGEKSRSRAGGGAGLGLAIAKGIVEA
                     HGGTIEVSSRVGEGSTFTFTLPA"
     gene            complement(4352..5062)
                     /locus_tag="AC162G_16365"
     CDS             complement(4352..5062)
                     /locus_tag="AC162G_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5077431.1"
                     /translation="MLAAMMNGQHILIVDDEPNVGDVLGIYLRRENFSVAVARDGKTA
                     LEEIERKPPDLMILDLMLPRVDGLQIVRHLRDYEQSDVPILILSAKTDEADRIVGLEL
                     GADDYVTKPFSPREVVTRVKALLRRAKNTTLVAEKPLGFGRLHIDPRTREVTVAGRAC
                     DLTAKEFDLLWFLARHPRQVFTRDQLLEHVWGFAEYVDPSTVTVHVRRLREKIEDDPS
                     DPQFVQTVWGVGYKFEAA"
     gene            complement(5160..5531)
                     /locus_tag="AC162G_16370"
     CDS             complement(5160..5531)
                     /locus_tag="AC162G_16370"
                     /inference="COORDINATES: protein motif:HMM:NF018021.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HIR5077432.1"
                     /translation="MDLDTFEQYVEEAVRGLPDVFRNRLHNVAIVVEEWPDRETMRVA
                     GIRRPVDLLGFYHGVPLPNRTHGYNAVTPDKISIYRSPILIHCRRSGENIRAMVHRVV
                     RHEIAHYFGIDDDRLQELGAY"
     regulatory      5697..5876
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6096..>6393
                     /locus_tag="AC162G_16375"
     CDS             6096..>6393
                     /locus_tag="AC162G_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077433.1"
                     /translation="MHRLIVPLALGMTTLMLALAALLTPAPAAAIGEMQQTTPQADSA
                     SANVGDVISKALAYIHSQQQPDGGIDAFASGASNPSGTARAVLALAAAGRPVDSM"
BASE COUNT         1223 a         2066 c         1946 g         1158 t
ORIGIN      
        1 gcgcacgctg gatgatttgc tgaccgcgtt ccatcgggcc ttgctgtcgg tgagccgaga
       61 agatgctatc gcgtggttcg ctcattgtgg ctacacgatg gcgtaaatca aataggaatt
      121 cgctctaatg tccgtgtaat ccgtgcgaaa tccgcgttaa tcgccggaga agtcgtcgtc
      181 gaagacaagg tcgcgccgag cctgacccgg ctgaacaaag cacagctcct ttcgtacctc
      241 aaagtgacgg gcaaacaagt cggattattg ctcaactttg gtgggccgat gccggagttc
      301 cagcggcttt tcttcgcgcc gcgcgagcct aaaatcgtcg acgcatacac cgagcgcgcg
      361 gtcagtaacg ttgagcgtcc ggatcttgtt gcgcccgaga tggtttacaa aatcataggc
      421 gggctgttcg aggtccacgc gacgctgggg ccgggctttg tccaccgcat ctacaccaac
      481 gcctgccacc acgagctcaa actgcgcgac ctaccggcca ggctacagaa gacgattcag
      541 gttttctacc gggacatctc gctcggtgaa ttgaagtttg gacacctctg cgtgggcgat
      601 gccgttatcg tcttcccgac cgcgatccaa agcatcgccg acctgaaact cgatgcgctt
      661 cgcgagtggc tgcgcgtgca agaaattcca ctcggcatcc tcgcgaactt caacgatacc
      721 gagctcaaac cgatcttcct gaagccacga agcgactgag ggacagcatc tgcaatccgc
      781 cgaattcacc cgatccgaag aattcacccg atccgctgtt aaggaccccc catgcgcctc
      841 gtcatccagc gtgtcaaaca agccagcgtc ttcgtcgacg gaaagatcgt cggccagtgc
      901 ggccacgggc tgtgcatcct ggccggcgtg cgcgtcggcg atacggaggc cgaagcgaag
      961 tggctggcac aaaaggtcgc cggactgcgc attttcgagg acgacgaagg caagatgaat
     1021 cgctctgcca tcgacgtggg cgggagtgcg ctcgtcgtct cacagttcac gctgtacgcc
     1081 gacgcgcgca agggccgccg cccctcgttc gtcgacgccg agctgcccga taaggccgcg
     1141 acgctcgtga tgaagttcgt cgaatacctg cgcgcggaag gcgtgccgac cgagaccggc
     1201 gtcttcggcg cgatgatgct cgtcgagatt cacaacgacg ggccggtcac catcattctg
     1261 gagcgggagg caggacagac caaagtaatt ggagattaga gattggcaat ctccaatctc
     1321 taatctctaa tccttcatct cccgtgtgat ggattgacga actgcgttaa gcatgttagt
     1381 atcctcgtaa ccaaatacag agagcgtggg gacgggcctc acgctctctt gcatgtgaaa
     1441 ggcagagatg ctcaaacgat ccgtcgcctg tgtggtcgtg ctcgtcgtaa cggcgtgcag
     1501 cacgacggcg ccaactaccg agccggcggc cacatccgaa ccgactgcga cacccaagcc
     1561 gacattgact tcgaccgccg cacaggcggc gtccctgacg ccaacagcga cgcctcagcc
     1621 ggcggtttct ccgaccgatc tgccgaccga cacgccgact gccaaaccga ctatccaacc
     1681 ggcagatacg gctgcgagcc agcctgccgc tcggccgacc aatccgcccg ctgcgcctcc
     1741 cgcgatcggc gacgccacgc cgaagccgcc gcccgggtcc ggctcgtgcg gaaactatcc
     1801 gtgcgatgac gacatcgccg gctgggtcaa tcggattcag gcaccgtccg ccttcaaagt
     1861 gtcgcacgtc gcccgcgcgc cgaacggcga gcacatcacg tcgatcacac acgggccgga
     1921 cggccgattg ttcgcgtcga cgatggaagg caacgtctac gtctttgatg cagcaggaaa
     1981 tccgtcgctc tacacgagtg gcttcaatct gccgatcggc ctggccttcc gtcctggcac
     2041 gagcgacctg tacgtcgcct cgcgtacgtt cgcgcccgat gccgtgccga acgaggggaa
     2101 ggtgacgatc gtgcgcccgg acggatcgaa gcaggacatc gtcaccggcc tgccgtgctg
     2161 ctacaccgcc gtcgagcacc agccgaacag tatcgccttc gggccggatg ggaaggtata
     2221 cctgagtatc ggcgcgcggg ccgatcatgg ggaggagccg ggaaaacctg acgtgcccgc
     2281 gacacttacg ccatacgaag cgggcatcct gcgcttcaac ctcgacggca gcgggctgga
     2341 gcggtacgcg gtcgggctgc gcaatccgta cgatctcgcc ttcgacgcca gcggccgcct
     2401 gttcgcgacc gacaacgggc ctgactacgg cccgcccgag cggctgcacg ccatcgtgca
     2461 gggcggccac tacggctacc cgtactacga ctgcgacatt tgccagaaag cgccgcccga
     2521 tctggccatc acgccgccga tcgcgcagtt catcgcgcac ggcgcgatca ccggtatcac
     2581 agcctacacg gcgaatgcgt tcccatcgaa ttattacaac gatctgttcg tcgtgctgtg
     2641 gagcgccttc cccggcgcgc agaaagtcgt gcgcgtatcg acgttcccga cggccgtcag
     2701 cgatttcgcg ctggggttcg cggcgccgat cgacgtgacc caatcgcctg acggcgcgct
     2761 gctcgtcgcc gactgggcga cggggcacat cttcaagatt gcctacagcc cgtaaatcta
     2821 ccgcaggtgg aacttctcag ccatcgtcat tgcgagcgtt tcttgcgaag caatctcatg
     2881 gccgaccggg ggattgcttc gtcgcaaaag gcgctcctcg caatgacggc tgagcaataa
     2941 cccgcagaga acgctgagag ggcggaagac aacgctatta accaccaagt cacgaagaca
     3001 ccaagttttc ttggtgtctt cgtgacttgg tggtgagatt cctcgctcgg tcaggcgggc
     3061 aaggtgaatg tgaacgtcga cccctcgccg acgcgactcg atacctcgat cgtcccgcca
     3121 tgcgcttcga cgataccttt ggcgatcgcc aggcccaagc ccgcaccgcc gcccgcgcga
     3181 gagcgcgact tctcgccccg atagaatcgc tcgaacacgc gcggcaggtc ttcggcggaa
     3241 atgccttctc ccgtatcgct caccgacact cgaaccttcc cgtcgtgccg ccgcgcgtcg
     3301 atcgtcacgt gcccaccctc cggcgtgtga cggagcgcgt tgctgatcag gttcgtcagc
     3361 acgcgctcaa tcttctgcgg gtcgatcgtc accgggtcga cctgcggcga cacctgccct
     3421 gccagccgca cctggcgccg ctcggcaagc gcgcgcatgc gctccagcac gtcggagatc
     3481 agatcgctca gcgaaaccga aaccttgtcg aacttcagcc cgcccgcgtc gagctgcgcc
     3541 agctcgaaca agtcgttgat caggctgttc aggctggcga cgtcagatcg gatcgagttc
     3601 agatagcgct ggatcgtttc aggatcgtcc accatgctat cggccagcgc ctcgacgacg
     3661 acgcggatac tggtgagcgg cgtgcgcaaa tcgtgcgacg tccacgcgat gaggtcgcgt
     3721 cgcagcgtat ctagctcgcg ctgcttgcgc gccgccgctt cgagctggtg agccatgtgg
     3781 ttgaaggtga tcgccagccg cgacacctcg tcgcgtccgt cgacctcggc gcgggccgag
     3841 aagtcgccac cggccaggcg ctgggcggcc tgctcgacgc gccgcaggcc gcgggtcagg
     3901 ctcgccgaca cgaacgcgcc gaacgagacg gcgatgcccg cgccgaacag cagcagcaca
     3961 ccggccagcg ccaggtcgtg gtagttgagg aacatgttgc gcgcggtgat ccacacgctg
     4021 atgaacaaca gcgcggcctg caggatcgac ccgaagatca tcgtgtgcag gatgcgcctg
     4081 ggccagcgcc accagccgag ccggtacagc acgtagcccg ccgcgaacgc acccaggctg
     4141 gtcagtgaca ggcccagcgc gagctgttcc aggtcggcgc gcggcggatg catcaccagc
     4201 tcgatggcga tgaccgcgac agccagcgtg aagatgacgc cgacgaggaa cgcggctggc
     4261 tgcccgatgc gttggaaacg gatggagagt gcgctcatgt ttcccattca cgtgcgacgc
     4321 acttcgcgag tgcgtcgcac gtaaagaaaa atcacgcggc ctcgaactta taaccgacgc
     4381 cccacacggt ctggacgaat tgcgggtcgc tcggatcgtc ttcgatcttc tcgcgcaggc
     4441 gccggacgtg aacggtgacc gtgctgggat cgacgtattc ggcgaacccc cacacgtgct
     4501 cgagcagttg gtcgcgcgtg aacacctggc gcgggtggcg cgccaggaac cacaacaggt
     4561 cgaattcctt cgccgtgaga tcgcaggcgc ggcccgcgac ggtcacttca cgcgtgcgcg
     4621 ggtcgatgtg caggcgtccg aaacccagcg gcttctcggc gacgagcgtc gtattcttgg
     4681 ctcggcgcag cagcgccttg acgcgcgtga ccacctcgcg cgggctgaag ggcttggtca
     4741 cgtagtcgtc cgcccctaat tccagcccga cgatacggtc ggcctcgtcg gttttggcgc
     4801 tgaggatgag gatcggcacg tcggactgct cgtagtcacg tagatggcgc acgatctgca
     4861 agccatcgac gcgcggcagc atcaggtcga gtatcatgag gtcgggcggc ttgcgctcga
     4921 tctcttcgag tgcagtcttc ccgtcgcgcg cgacggcgac gctgaagttc tcgcggcgca
     4981 ggtagatgcc cagcacgtcg ccgacgttcg gctcgtcgtc cacgatcaag atgtgctgcc
     5041 cgttcatcat cgctgccagc atgcctccag cctagcggct gaggcttaca cggagattac
     5101 gcgccgggac gaatgacgaa ggacagacga cgatggcatg tgtctttggt cattcgtctt
     5161 caatacgccc ccagttcttg caggcggtca tcgtcgatcc cgaagtagtg agcgatctcg
     5221 tgccggacga cgcgatgcac catcgcgcga atattctccc cgctgcgccg gcagtggatc
     5281 aggatcggcg accggtagat gctgatcttg tcgggcgtga cggcattgta gccgtgcgtg
     5341 cggttcggaa gcgggacgcc gtggtagaag ccgagcaggt cgaccggacg cctgatcccg
     5401 gcgacgcgca tcgtctcgcg gtcgggccat tcctcgacga cgatcgcgac gttgtgcagc
     5461 cggttccgaa agacgtcagg caggcctcgc acggcttcct ctacatattg ctcgaaagtg
     5521 tcgagatcca tcgccagcct attgtagcag caactttgct atctggcata gcacaaccca
     5581 ttccgcatct cgatcaaaag ggaggtccgc caataacgcg aatccccgca aattcatgcg
     5641 tattgaccca cgcttgacgg cgaagggtgt ccatgttaaa cttgtcccgt ctttaataac
     5701 cgaacatcgg caaaggtgcg ccagacgccc ggatgcgtgg cgcacaaggg gggaagccgg
     5761 tgacattcgc gccatatccc aggatatgcg agtgaagtcc ggcgctgtcc cgcagcggta
     5821 gcactgctct gatggagcgg tggagcccga atacccgcct ttgcccggct ttgcgttccc
     5881 ttcgcggaaa ggggggccac gttgcgctct cccgccctca aggcgggttt tttttcttgc
     5941 ggcctcacct tcaaggtagg ccgcatgctg ttttgacgcc aagacttcgc gccgcgaagg
     6001 cgcaaagggc accaagactt cggaagtcct taagtggcat ctcgccggcg aaatgcgcct
     6061 taagacttcc gaagtctaaa aaggagacat attccatgca caggttgatt gtacccctag
     6121 ctctgggaat gacgacgttg atgctcgcgc tggctgcgct gctgaccccg gcgcccgccg
     6181 ccgcaattgg cgagatgcag cagactaccc cccaggcgga ttctgcctcg gccaacgttg
     6241 gcgacgtgat ctccaaggcg ctggcttaca ttcactcgca gcagcagcct gacgggggaa
     6301 tcgacgcttt cgcttccggc gcttcgaatc caagcggcac ggcccgcgcg gtgctggctc
     6361 tcgccgccgc cggccggcct gtcgattcga tga
//
LOCUS       DBNHXB010000411         3330 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4148199, whole genome shotgun sequence.
ACCESSION   DBNHXB010000411 DBNHXB010000000
VERSION     DBNHXB010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4148199"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="AC162G_16380"
     CDS             <1..201
                     /locus_tag="AC162G_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077434.1"
                     /translation="PAPSSAKSSTISKKSSWKPADDVFVAVSADPPLMYGLLKADMPI
                     RPRVKLALGQLRVRASTAPPRE"
     gene            203..1090
                     /gene="iolB"
                     /locus_tag="AC162G_16385"
     CDS             203..1090
                     /gene="iolB"
                     /locus_tag="AC162G_16385"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HIR5077435.1"
                     /translation="MPSSRDLLLHPDPNAAEYACVDPQRAGWEHLHFAARRLRAGQRW
                     THNTGETEMALVVLGGVCSLAWQKDSARGEWSEIGRRPNVFSGMPYAIYLPRRTEFHL
                     EARSDDFDMAYGWCATDEDHSPARVTPDQVTIEIRGGANATRQINGILNPGFDCHRLV
                     AVEVYTPSGNWSSYPPHKHDQHRVGHDGALFEADLEEVYFYKIDRPEGYAIQRVYTQD
                     GRLDETLVARNDDVVLVPEGYHPVVSAHGYTTYYLNILAGSAQSLANSDDPDYAWIKD
                     TWTWKDHRLPLVSMDMERR"
     gene            1106..2077
                     /gene="iolC"
                     /locus_tag="AC162G_16390"
     CDS             1106..2077
                     /gene="iolC"
                     /locus_tag="AC162G_16390"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HIR5077436.1"
                     /translation="MRQSEKRFDILTMGRCAVDFYCNDIGVALKYARSFNVYVGGCPA
                     NVAVGTRRLGLRSAILTRVGIEPLGDFILHFLETEGVNTEAIVRDPDHLSGLVVLGIE
                     PPDTFPMTFYRADCADINLTLDDVRAAPIGDAHVLFVSGTGFSREPSASATMAAVEIA
                     RASGSTVMLDIDYRPTLWDDPRAFGPVLRRIVAEVDLAAGTEDEIRAAADEPDVDAAT
                     ERLLRLGRNLQALIVKRGQRGSTVFAAGGARIDAPPFPVRILNVIGAGDAFVSGFVYG
                     YRRGWDWRKCARFGNATGAIVVTRHACANDMPYEPEALDFVAAGGGF"
     gene            2074..3219
                     /locus_tag="AC162G_16395"
     CDS             2074..3219
                     /locus_tag="AC162G_16395"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5077437.1"
                     /translation="MNEPLRVGISGCGNIALNDHAPALKSLDGARVVAIADPVEARRS
                     KVQALLGLPDSACFADHRALLDAGVDYVTLTVPQKFRRPIVEDCARADVHVLAEKPIA
                     TVPADARDMIRLMRDANLRYGMVHNYLFYPEYALAKDLIDSGAIGTLRHLTLNFLGMP
                     DHPGAAEYRPQWRKDPVEAGGGILMDMVHAVYLAEFFFGGPIRAVSAIVDNLDHPGDA
                     VEDFTLVDYAFDTGYATVNMWWGGGPGGLEIGGTQGRILVFYDNYATGPFTRLAAFTL
                     VNSRGRQEFQPRAERRTVENFVRVHADFIEAIRDGRDPFAPAEAGLRSIEAVLGAYAS
                     GATGRVVALPLSSDGPVFRRGVIGLRELALWADGPVFKRNIFGVKTP"
BASE COUNT          564 a         1099 c         1093 g          574 t
ORIGIN      
        1 ccggcccctt cctcagcgaa gtcatccacg atatcgaaga agtcgtcttg gaagccggct
       61 gatgacgtgt ttgtcgccgt gtctgccgac ccgccgctga tgtacgggct gctaaaagcc
      121 gacatgccca ttcggccgcg cgtgaagctt gccctcggcc agttgcgggt acgtgcctcg
      181 acggcgccgc cgcgagagtg acatgccgtc cagccgagac ctgctcctgc atcccgaccc
      241 gaacgctgcc gaatacgcgt gcgtcgatcc acagcgcgcc ggctgggaac acctgcactt
      301 cgccgcccgc cgactacgcg ccgggcagcg ctggacgcac aacacgggtg agacagagat
      361 ggcgttggtc gtcctcggcg gtgtgtgcag tctcgcctgg cagaaagact cggctcgcgg
      421 cgagtggagc gaaatcggcc gccgcccgaa cgtcttcagc gggatgccct acgccatcta
      481 cctgccgcgc cgcaccgagt ttcacctcga agcgcggagc gacgacttcg acatggctta
      541 cggctggtgc gcgaccgacg aagatcattc acccgcgcgg gtgacgccgg atcaggttac
      601 gatcgagatt cgcggcggcg cgaacgccac gcggcagatc aacggcatcc tcaaccccgg
      661 cttcgactgc catcggctgg tcgcggtcga ggtgtatacg ccctccggca actggagctc
      721 gtatccgccg cataagcacg atcagcaccg cgtcgggcac gacggcgcgt tgttcgaagc
      781 cgacctcgaa gaggtctact tttacaaaat cgatcgaccc gaaggttacg cgatccagcg
      841 cgtgtatacc caagacggcc ggctcgacga aacccttgtc gcgcgcaacg acgatgtggt
      901 gctcgtgccg gaagggtatc acccggtcgt ctcggcacac ggttacacga cgtactacct
      961 caacatcctg gccggttccg cgcagtcgct cgctaacagc gacgaccccg actacgcctg
     1021 gatcaaagac acgtggacgt ggaaggacca cagactgcct ctggtgtcga tggatatgga
     1081 gcgtcggtag atcgagccgc acaacatgcg ccaatccgaa aagcgtttcg atatcctgac
     1141 catgggccgt tgcgccgtcg atttctactg caacgacatc ggcgtggcgt tgaagtacgc
     1201 caggtcgttc aacgtttacg tcggcggctg cccggcgaac gtcgcggttg ggacacggcg
     1261 gctgggcctg cgatcggcga tcctgacgcg ggtcggtatc gagccgctgg gcgacttcat
     1321 cctgcacttc ctggagacgg agggcgtgaa cacagaagcc atcgtgcgcg accccgatca
     1381 cttgagcggg ctggtcgtgc tcggcatcga gccgcccgac accttcccga tgacgtttta
     1441 ccgcgcggac tgcgccgaca tcaatctcac gctcgacgac gtgcgcgccg cgccgatcgg
     1501 cgacgcacac gtcctgtttg tgtcgggtac aggcttctca cgcgagccga gcgccagcgc
     1561 gacgatggcc gcggtcgaga tcgcgcgcgc cagcggctcg acggtaatgc ttgacatcga
     1621 ctatcgtccc acgctgtggg acgacccgcg cgcattcggt ccggtgctgc gccgcatcgt
     1681 cgccgaagtc gatctggctg ccgggacaga agacgagatc agggcggccg cagacgagcc
     1741 ggacgtcgat gcagcgacgg agcgccttct acggctcggc cgcaacctcc aggcgttgat
     1801 cgtcaagcgc ggccagcgcg gctcgacggt attcgccgcc ggcggcgcac gcatcgacgc
     1861 gccgccgttt ccggtccgga tcttgaatgt gatcggcgcg ggcgacgcgt tcgtgtcggg
     1921 ttttgtgtac ggctaccggc gcggctggga ctggcgcaag tgcgcgcgct tcggcaacgc
     1981 gacgggcgcg atcgtcgtga cccggcacgc ctgcgccaac gatatgccct acgagccgga
     2041 agcgctcgac ttcgtcgccg ctggaggtgg cttttgaatg aaccgctcag agtcggcatc
     2101 agcggctgcg gtaacatcgc gctgaacgac cacgcgcctg cgctgaagtc gctggacggc
     2161 gcacgcgtcg tggcgatcgc cgatccagtc gaagcgcggc gcagcaaggt tcaggcgctg
     2221 ctgggtttgc cggactcggc atgcttcgcc gatcaccgcg cactgctcga cgctggcgtc
     2281 gattacgtga cgctgaccgt gcctcagaaa ttccgccgcc cgatcgttga agattgcgcg
     2341 cgggcggacg tccacgtgct ggccgagaaa ccgatcgcca ccgtcccggc cgacgcgcgc
     2401 gacatgatcc ggctgatgcg cgacgcgaac ctgcgctacg gcatggtgca caactatctc
     2461 ttctatcccg agtacgcgct ggcgaaagac ctgatcgatt cgggcgcgat cggcacactg
     2521 cgccacctca cgctgaactt cctagggatg cccgatcatc ccggcgcagc cgagtatcgc
     2581 ccgcagtggc gcaaagaccc ggtcgaggct ggcggcggca tcctgatgga tatggtgcac
     2641 gccgtttacc tggccgagtt cttcttcggc gggccgatcc gcgccgtgag cgcgatcgtc
     2701 gacaacctcg atcatcccgg cgacgcggtc gaagacttca cgctcgtgga ctacgcgttc
     2761 gacacgggat acgccacggt caatatgtgg tggggtgggg ggccgggcgg gctggagatc
     2821 ggcggcacgc aggggcgcat cctggtcttc tatgacaact acgccaccgg cccgttcacg
     2881 cggctggcgg ctttcacact cgtcaacagc cgggggcggc aggagttcca gccccgggca
     2941 gagcggcgca cagtcgagaa ctttgtgcgc gtccacgccg acttcatcga ggcgatccgc
     3001 gatggccgcg atccgttcgc gcccgccgag gccggcttgc gctcgatcga agccgtgctc
     3061 ggcgcgtacg cttcaggcgc gaccgggcgg gtggtggcgc tgccgctgtc atccgacggt
     3121 ccggtgttcc ggcgcggcgt gatcggtctg cgcgagctgg cgctgtgggc ggatgggcct
     3181 gtcttcaagc gaaacatttt tggcgtgaaa accccgtagg gccggcatcc ccatgccggc
     3241 cctcacccgc ggaggccatt ttgaaaatcg gactgttcac cgacgttctg caagatctgt
     3301 cgttcgccga agcgctggat tgggtggccg
//
LOCUS       DBNHXB010000412         3739 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4483418, whole genome shotgun sequence.
ACCESSION   DBNHXB010000412 DBNHXB010000000
VERSION     DBNHXB010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4483418"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="AC162G_16400"
     CDS             complement(<1..617)
                     /locus_tag="AC162G_16400"
                     /inference="COORDINATES: protein motif:HMM:NF025067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HIR5077438.1"
                     /translation="MTHATKLGLTLLGAALALGVLGDALLRATPWGLNVVLWIGPLVA
                     VGILLASRHEVALTGGGRWLIAPAIFFAAVLAWRDSPTLNWLNLSALLAALALVALRA
                     RAGRVLIAGVLEYVQGFLVAAVGWAIGLFELLVDDIKWKEMPSRDWSKQATAAGRGLA
                     IALFLLFVFGGLFVAADAVFEDFVARLFDWDVDEVVTRTILISFWT"
     gene            complement(835..1974)
                     /locus_tag="AC162G_16405"
     CDS             complement(835..1974)
                     /locus_tag="AC162G_16405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013144479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR5077439.1"
                     /translation="MKDVVIVEAVRSPIGKRDGKLSSVLPVHLMARVLQALLARSGID
                     PALVEDVILGCVTPIGEQGANIARLAALQAGLPMHVPAASLNRMCGSGQQAIHFAAQV
                     IAAGDMDVVIAGGIEVMSRVPLGSDWPKAWPPDFPYALVSQGISAEMMAEKWHLTREE
                     LDCFAYESHVKAAAATRRGDFAREIVPIEVKLPDGVTDTLTYDEGIRFEPDLGKMSKL
                     QPVFKEGGVITAANSSQISDGAAAVLLMSADKASALGLKPRARIAARYVIGDDPVLML
                     SAPIPATRAALERAGLRLDDLSVIEINEAFASVVLAWQKELQPDPARVNPNGGAIAMG
                     HPLGATGAILMTKLLHHLERAGDRYGLQTMCIGHGMATATVIERV"
     gene            complement(2054..2734)
                     /locus_tag="AC162G_16410"
     CDS             complement(2054..2734)
                     /locus_tag="AC162G_16410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5077440.1"
                     /translation="MRLSVIIPVYDEKATIAEILRQVRAVGLASEIIVVDDGSTDGTR
                     DVLKGEEPTPNTIVVYHDRNRGKGAAVCSGLERATGDVILIQDADLEYDPRDYPALIR
                     PIEEGRVKVVYGSRFLGPRKAMLFWHMLGNKMLTLMTNILYNAILSDMETGYKVFRAD
                     VIKNIPLKSKRFEFEPEITAKVLKRGHRIFEVPISYYGREYHEGKKITWREGPNAIWT
                     LIKYRFVD"
     gene            complement(2791..3384)
                     /locus_tag="AC162G_16415"
     CDS             complement(2791..3384)
                     /locus_tag="AC162G_16415"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010272174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR5077441.1"
                     /translation="MAAPTEHSLRRCAWAGSDLRMVAYHDEEWGVPLHDDRRLFEFLV
                     LEGMQAGLSWSTVLRKRPNFREAFHGFDPVRIARYTAADVRRLLANPGIIRNRLKIEA
                     AVNNARRFLEVQAEFGSFDNYIWQFVDGKPIRNRHKSLAEIPSRTPLSDNISKDLQKR
                     GFKFVGSTIVYAHMQATGMVNDHLVDCFRYKEVMQEA"
BASE COUNT          738 a         1157 c         1089 g          755 t
ORIGIN      
        1 gtccagaacg agatcaagat cgtgcgcgtc acgacttcgt cgacgtccca gtcgaacagc
       61 cgggcgacga agtcttcgaa cacggcgtcc gccgcgacga acaggccgcc gaagacgaac
      121 agcaggaaca gcgcgattgc cagcccgcgc ccggcagcgg ttgcctgctt cgaccagtcg
      181 cggctcggca tctccttcca cttgatgtcg tcgacaagca gctcgaacag gccaatcgcc
      241 catccgacgg cggcgaccag gaaaccctgg acatattcca gcacgcctgc gatcagaact
      301 cgtccggccc gcgcgcgcag cgcgaccagc gccaatgcgg cgaggagcgc cgacaggttg
      361 agccagttca gggtgggcga atcgcgccag gcgagcacgg cggcgaagaa aatcgcgggg
      421 gcgatcagcc aacgcccgcc gccggtcagc gcaacctcat gcctagaagc aagcaggatg
      481 cccaccgcga cgagcgggcc gatccacagg acgacgttca gcccccacgg cgtcgcgcgt
      541 agaagggcat cgccgaggac accgagcgcc agtgcggcgc cgagcagcgt aaggccaagc
      601 tttgtcgcat gagtcatcgt cttcccttcc aattccaacg gtcagaccgt cgcactacag
      661 gtatggtatg ggatgattcc tctccatctg ctcctcgaaa tagagtaaac tgaagcggcg
      721 gcaaagaaca cgcttcgcgt acgcggtgaa ttgcactcgc gtgcttgaca aaaagacctt
      781 tcgggttttg aaagcccgaa gggtctccaa cgggatgggt ctccgacgag tttctcaaac
      841 cctctcgatg acggtcgccg tcgccatacc gtggccgatg cacatcgtct gcagaccgta
      901 gcggtctcct gcccgttcga ggtgatgcag cagcttcgtc atcaggatcg ccccggtcgc
      961 gccgagcgga tgccccatcg cgatcgcgcc gccattcgga ttgacgcgcg ccgggtccgg
     1021 ttgcagctcc ttctgccagg cgagcacgac cgaggcgaac gcctcgttga tctcgatcac
     1081 cgaaaggtcg tcgaggcgca agccggcgcg ctcgagcgcg gcgcgcgtgg cggggatcgg
     1141 cgcggagagc atgagcaccg ggtcgtcgcc gatcacatag cgagcggcga tccgtgcgcg
     1201 gggcttgagt ccgagcgcgc tcgccttgtc agccgacatg agcagcaccg ccgccgcgcc
     1261 gtcgctgatc tggctggagt tggcagcggt gatgacgccg ccctctttga agacgggctg
     1321 gagcttgctc atcttgccca ggtcaggctc gaagcggatg ccttcgtcat aggtcagcgt
     1381 gtcggtcacg ccgtcgggca gcttcacttc gatcggcacg atctcgcgcg cgaagtcgcc
     1441 gcgcctcgtc gcagccgccg ccttgacgtg gctctcgtac gcgaagcagt cgagttcttc
     1501 gcgcgtgaga tgccacttct cggccatcat ttcggcggag atgccctggc tgacgagcgc
     1561 gtatggaaag tcgggcggcc acgctttcgg ccagtccgag ccgagcggca cgcggctcat
     1621 gacttcgatt ccaccggcaa tgacgacgtc catgtcacct gccgcgatga cctgcgcggc
     1681 gaagtgaatc gcctgctggc ccgatccaca cattcggttg agtgacgcgg cgggcacgtg
     1741 cattggcagg cccgcctgca gggcggccag ccgcgcgatg tttgcgccct gctcgccgat
     1801 cggcgtcacg cagccgagga tgacatcttc gacgagcgcc ggatcgatgc cagaacgcgc
     1861 gagcagcgcc tggagcacac gcgccattag gtgaaccggc agcacggaac tcaatttgcc
     1921 atcccgcttg ccgatgggcg agcggacggc ttcgacaatg acgacgtctt tcatagggag
     1981 cctccggaga taaaacgtga tgcgtgatgc gtggtacgtg atgcgtgaat cagcattcta
     2041 cattctacat tccttagtca acgaacctgt atttgattaa ggtccagatc gcattcggcc
     2101 cttcgcgcca ggtgatcttc ttgccctcgt gatactcgcg gccgtagtac gagatcggca
     2161 cttcgaagat acggtggccg cgcttgagca ccttggccgt gatctccggc tcaaactcga
     2221 agcgcttgga cttgagcggg atgttcttga tcacgtcggc ccggaagacc ttgtagccgg
     2281 tctccatgtc gctgagaatc gcattgtaaa gaatgttcgt catcagcgtc agcatcttgt
     2341 tgccgagcat gtgccagaag agcatcgcct tgcgcgggcc gaggaagcgc gagccgtaca
     2401 cgaccttgac gcggccttcc tcgatggggc ggatcagcgc cggatagtcg cgcgggtcgt
     2461 attccaggtc ggcgtcctgg atgaggatga cgtcgcccgt cgcgcgctcc aacccgctgc
     2521 acacggcggc gcccttgccg cgattccggt cgtgatacac tacgatcgtg ttcggcgtgg
     2581 gctcctcgcc tttcaagaca tcgcgcgtgc catccgtcga gccgtcgtcg acgacgatga
     2641 tctcagacgc cagcccgacg gcgcgcacct ggcgcagtat ctcggcgatg gtggctttct
     2701 cgtcgtagac cggaatgatg accgataggc gcatgccata ccccttcatc cctcacgcat
     2761 cacgtaatca cgtaatcacg tgattacgta tcacgcctcc tgcattactt ccttgtacct
     2821 gaaacagtcg accagatgat cattcaccat gcccgtcgcc tgcatgtgcg cgtacacaat
     2881 cgttgagccg acgaacttga agccgcgttt ctgcaggtct ttgcttatgt tatctgacag
     2941 cggtgttctc gatggaatct cagctaagga tttgtgacgg ttcctgattg gcttgccgtc
     3001 gacaaactgc cagatgtagt tatcaaagga gccaaactcc gcctgcactt caaggaaacg
     3061 ccgggcgttg ttgacagccg cctcaatctt cagccgattg cggatgatgc ccggattggc
     3121 cagcaggcgt cgcacgtccg ctgccgtata tctggcaatt ctcacggggt caaacccatg
     3181 gaatgcctca cggaagttgg gccttttgcg aagcactgtt gaccagctaa gtccggcttg
     3241 catcccctcc aacacgagaa attcaaacaa cctgcggtca tcgtgcaacg gaacgcccca
     3301 ctcctcgtca tggtaagcaa ccatacgaag atcgctaccg gcccaagcgc atcgacgcag
     3361 gctatgctct gtaggtgcag ccataggctt tgcctccaat gccgtttcct gtgcttccaa
     3421 agtacgtccg attgtatggg caacagtgac ttgaggcaag ttgagagtct tggctataat
     3481 acgggtagtg acttagatag agcagtagat acttttacca cccaacctat acttaggttt
     3541 tgccgctttt tgaattttgc atgctgttga tataatcgtg ccacgcgttc gcatttcctc
     3601 cacggcgcgt tcacaatgca ttcacaattg tgaggcatac tcctgttcag attgaatttt
     3661 ggggtgtgta ttttataatt gatctatgtc tacgccaatt ttagccacta aactttacac
     3721 gcccccacct cggcctaaa
//
LOCUS       DBNHXB010000413         7729 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_794199, whole genome shotgun sequence.
ACCESSION   DBNHXB010000413 DBNHXB010000000
VERSION     DBNHXB010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7729
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_794199"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="AC162G_16420"
     CDS             <1..236
                     /locus_tag="AC162G_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR5077442.1"
                     /translation="LSGSLDRVPTEADPIFGLHMPVECPGVPSEVLNPRRTWPDPNAY
                     DAKARELASLFVKNFEQYADQAAPEVKAAGPRP"
     gene            239..754
                     /locus_tag="AC162G_16425"
     CDS             239..754
                     /locus_tag="AC162G_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077443.1"
                     /translation="MGILTHQLDGAIVGYDLELLTKGVLVTGPGFADVAARLVIDGGR
                     LIQQGSLTLRLGVLLYAEPYLFIPQSIVDEVGQTIAGETVFDWFGANAYTMPRAQVFG
                     IDAAGKGDMIFARDVDVESSPIVLGGLDASPVYVAFVHGQTPFPPRFAKALRSLPAEQ
                     LPEVPRRIASA"
     gene            1100..1291
                     /locus_tag="AC162G_16430"
     CDS             1100..1291
                     /locus_tag="AC162G_16430"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR5077444.1"
                     /translation="MIKVTYRDKKWEVPGRITVRDLIVKVGLNPETILAVKNGKLIND
                     GTLLGDEDDIRLIAVISGG"
     gene            1305..2267
                     /locus_tag="AC162G_16435"
     CDS             1305..2267
                     /locus_tag="AC162G_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)-lysidine synthetase"
                     /protein_id="HIR5077445.1"
                     /translation="MRCRKCGELAVINMRQHRLALCETHFVEWMRANTQRFVEKYRMF
                     KPDERVLVAVSGGKDSLALWDILLALGYNADGLYLGLGIDGGIGYSDASRVCAEKFVR
                     DFWPDARLHVLDVKGAYGESIPEVAQRSNRGRGKPCSVCGLIKRHEMNRIAYDLGYDV
                     LATGHNLDDEAAVLWGNTLHWKTEYLARQAPVLPADRPGFARKVKPLCRFYERETAAY
                     ALVRGVDYIYDECPCAAGATSITYKELLNQMEKDRPGAKLDFYLSFLRAKEDGLLAQV
                     GASTELHACDRCGQPTSAPGLCAFCRLWEGHAVPDEGPNVVIVG"
     gene            2338..3051
                     /locus_tag="AC162G_16440"
     CDS             2338..3051
                     /locus_tag="AC162G_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160254 - tRNA queuosine(34) biosynthetic
                     process from salvaged queuosine or its precursors
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HIR5077446.1"
                     /translation="MRSYKYLSWVTAIFVTCLITANIIAVKLIDVGGLILPAAVIIFP
                     VSYIVGDVLTEVYGYARARQVIWIGFACNLIAVVAIAIAGVLPPADFWASNQAAYETI
                     LGFTPRLLAASFVAYLVGEFLNSYVLARMKVATEGRYLWTRTIGSTLVGQFADSAVFI
                     TLGLLQITALPPAMQGTLPPQALLNTIVVQWLFKSLYEIVATPLTYVVVGRLKRAEGA
                     DVFDRDTNFSPFHLQQVDG"
     gene            3081..3488
                     /gene="queF"
                     /locus_tag="AC162G_16445"
     CDS             3081..3488
                     /gene="queF"
                     /locus_tag="AC162G_16445"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HIR5077447.1"
                     /translation="MEKSELLKDLTLLKRDAAKPMRKLETFPNLHPGRPYTITLESNE
                     FTCVCPATGQPDFATITIRYVPDQRIVESKSLKLYLWSFRDEGHFHEHVTNIILDDLV
                     AALEPLRCEVIGKFNVRGGIAITVEAKYERPVP"
     gene            3562..4707
                     /locus_tag="AC162G_16450"
     CDS             3562..4707
                     /locus_tag="AC162G_16450"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR5077448.1"
                     /translation="MSYEDAHKLLRRNRELSILNAIAEALNRSVDLPQALEGVLRLVA
                     ELLSLRTGWIWLLDEDTGEPYLAAAQFLPPALANEPERMTGSCYCLDTFEQGDLTGAA
                     NVNVVTCSRLKALIDGTDGLRYHASIPIYSHTDKLGVLNVASADWRELDADDLRLLYT
                     IGYQLGVAIERARLYARSAQLAKIEERNRLAREIHDTLAQGLTAISLQLESADALIDA
                     SPARAKDKLRKALELARLNLEEARRSVLDLRAAPLLERSLPDALRALADSIARERGLS
                     IDVQVQGAVDGMPGRIEAGVYRIAQEALNNAARHSSAKRIVVSLSRSDDRLRLAVEDD
                     GRGFEPQAATTGFGLIGMRERARLLGGTLEVDSALNRGTRILLEIPV"
     gene            4813..5454
                     /locus_tag="AC162G_16455"
     CDS             4813..5454
                     /locus_tag="AC162G_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5077449.1"
                     /translation="MITIFLADDHPVVRDGLRAILETQPDFAVIGEAGDGAETVWQTK
                     ALRPDVLLLDLEMPEMDGVEALRHISEAAPDTRVLVFTAYDSDERILAAVQAGVQGYL
                     LKGAPRDQVFDAIRVVHGGGSLLQPAVASKLLKQVQSGTDRTGSRAGELTSREIEVLR
                     LLAQGQANKQIAARLGISERTVKFHVSAIFRKLDATNRTEAVTIAAQRGLIKL"
     gene            5612..7420
                     /locus_tag="AC162G_16460"
     CDS             5612..7420
                     /locus_tag="AC162G_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077450.1"
                     /translation="MSTLAQVTSAVSFLSMAASLWLGCYVVTRSPRSRLAWQAGLTLW
                     SLAGLFVTILLAIGDSTSPSRWPRWPLTLTLAIWYHLSLETLPPERRTTQRRFLPVVY
                     GLAALLDVLLLQTSLIASDRMPGVGAYTTVFERRPLFAIWPIFLIGLALLMLYNFWTA
                     RRAVRDRMHRKQLNSLVRGTFLGVLAAVYWTVAISLGVSVPALPIVLALGLGVGMLGH
                     GIARYSALIDGRILRFDFDLSALLMLALSAIYLAATWFLFQPAWSIAAAIVVVALTIF
                     THTSFEFARRLLDRPFVRLPARALRATLRRAASDLGERKSVEESLRSALAALVVGVNA
                     RWGAIALRDGGEFVVRASFHGERVGERLPAHHLNVRELTVLSPEGSPDWPLAVIAPMI
                     ADRDAVGAILLGQPRGGTHYSEGDLDLVAEAADRLAELIRYAQEHEAHARQIGNMLEA
                     FRAREYELQSGIDGLLYQAKAAAPSPQQVAEVENAMRRLYDYSYLGEHPLARNMLAHD
                     HATTHIDRGKALNAVLVTAIEKLRPAAVEPRELPPREWHPYLILRDAYLRGDANRDIM
                     SRLYVSEATFHRTRRGALRAVTKALHEMDLTPVQAD"
     gene            7494..>7729
                     /locus_tag="AC162G_16465"
     CDS             7494..>7729
                     /locus_tag="AC162G_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077451.1"
                     /translation="MSHKMSVTAPIRGVTDRVRAAVDRFRQRTSSNRLIAALRRAIPS
                     QGLLSVWWRSILNLVRLLRNLLTLLVLVPLRGLY"
BASE COUNT         1345 a         2425 c         2470 g         1489 t
ORIGIN      
        1 cgctgagcgg gtcgttggac cgggtgccca ccgaggccga cccgatcttt ggcctccaca
       61 tgccggtcga gtgccccggc gtcccgtccg aggtgctcaa tccgcgccgc acgtggcccg
      121 acccgaacgc ctacgacgcg aaagcgcgcg aactggcttc gctgttcgtc aagaacttcg
      181 agcagtacgc cgatcaagct gccccggaag tgaaggcggc cgggccgcga ccttagaaat
      241 gggaatcctg acccatcagc tcgacggcgc gatcgtcggc tatgacctgg agctcctgac
      301 gaaaggtgtt ctggtcaccg gaccaggctt cgccgatgtc gccgcccggc tcgtcataga
      361 cggcggtcgc ctgatccagc agggatcgct gacgctgcgg ctcggcgtct tgctctacgc
      421 cgagccttat ctgttcatcc cccagtcgat cgtcgacgaa gtggggcaaa cgatcgccgg
      481 cgagaccgtc ttcgactggt tcggcgcgaa tgcctacacc atgccgcgcg ctcaagtctt
      541 cggaatcgat gccgccggaa agggtgatat gatcttcgca cgcgacgtgg acgtcgagtc
      601 tagccccatc gtcctcggcg gcctggatgc ctcgccggtc tacgtcgcat tcgtgcacgg
      661 tcagacgccg ttcccgccga gatttgccaa agccctgaga tcgctgccgg cggaacaact
      721 gcccgaagtt ccgcggcgca tcgcgtcggc gtagtcggga agtgaccgaa gaggtcctcg
      781 ggaggggcgg cgttcagccg cccctcttct ttgcaactgc cttgacgcat ccttgctttc
      841 aacttagaat cgtcatgctt ctgagagtgc gtcttaagtc tgctcgaagc ccgcgtccct
      901 tcggctgccc cttcgctctg ctcagggctt cggtttcccg ctccaaccgc ccctgccggg
      961 ggttcggtcg gagacttcgc agagcctgcc ctgagcgcag cgaaggggca ggcttggttc
     1021 agggcaagcc tcgtgggcgc gctccgcagc catcctcgac ggcgcaggct gaaatgggat
     1081 gtctggatgg gaggtgagca tgatcaaagt gacttaccgc gacaagaagt gggaagtccc
     1141 tggccgcatc actgtgcgtg acctgatcgt caaggtcggt ttgaatcccg agaccattct
     1201 cgccgtcaag aacggcaagc tgatcaacga cggcacgctg ctcggcgacg aggatgacat
     1261 caggctgatc gccgtgattt cgggcgggtg acacgatgaa agggatgcgc tgccgcaagt
     1321 gcggcgagct tgccgtcatc aacatgcgcc aacaccggct ggcgctgtgc gagacgcatt
     1381 tcgtcgaatg gatgcgcgcg aatacgcagc gcttcgtcga gaaataccgc atgttcaagc
     1441 cggacgagcg cgtgctcgtc gccgtgtcgg gcggcaaaga tagcctggcg ctgtgggaca
     1501 tcctcctcgc gctcggctac aacgccgacg gcctgtatct cggcctcggc atcgacggcg
     1561 ggatcggcta ttcggacgcc tcccgtgtct gcgccgagaa gttcgtgcgc gatttctggc
     1621 cggatgcgag gctgcacgtg ctggacgtga aaggcgcgta cggcgagtcg atccctgagg
     1681 tcgcgcagcg cagcaatcgc gggcgcggca agccgtgctc ggtgtgcggt ctgatcaagc
     1741 ggcacgagat gaaccgcatc gcctacgacc tgggctacga cgtgctggcc accggccaca
     1801 acctcgacga cgaggcggcg gtgctgtggg gcaacacgct gcactggaag acggagtacc
     1861 tggcgcggca ggcgccggtc ttgccggccg atcggcccgg cttcgcgcgc aaagtcaagc
     1921 cgctgtgccg cttctacgag cgcgagacgg cggcgtatgc gctcgtgcgc ggcgtcgact
     1981 acatctacga cgagtgcccg tgcgccgccg gcgcgacgag catcacctac aaagaattac
     2041 tcaaccagat ggagaaagat cggcccggcg cgaagctcga tttctatctc agcttcctgc
     2101 gcgcgaaaga ggacgggctg ctggcccagg tgggggcttc gaccgagctg cacgcgtgcg
     2161 atcgctgcgg ccagccgacc agcgctcccg gtttgtgcgc gttctgccgt ctatgggaag
     2221 gccacgcggt gccagatgaa gggccgaatg tggtgattgt gggttagcgg cgttcctctc
     2281 gctgattcgc tgactcactg attcgctcat caacaggggc gcttatggaa acctctcatg
     2341 cgatcttata agtatctatc gtgggtcacg gccattttcg tcacgtgcct gatcacggcg
     2401 aacatcatcg ccgtcaagct gatcgacgtc ggcgggctga ttctgccggc ggcggtcatc
     2461 atctttcccg tctcgtacat cgtcggcgac gtgctgaccg aggtatacgg ctacgcccgc
     2521 gcgcggcagg tgatatggat cggcttcgcg tgcaacctga ttgccgtcgt cgctatcgcc
     2581 atcgcgggcg tgctgcctcc ggccgacttc tgggcgtcca accaggcggc ctatgaaacg
     2641 atcctgggct tcacgccgcg cctgctcgcg gcttcgttcg tcgcctacct ggttggcgag
     2701 tttctcaact cgtacgtgct ggcgcgtatg aaggtcgcca ccgaaggccg gtacctctgg
     2761 acgcggacga tcggcagcac cctggtcggg caatttgccg attcggccgt gttcatcacc
     2821 ctcggcctgc ttcaaatcac ggcgctgccg cccgccatgc agggcacgct cccgccccag
     2881 gcgcttctca acacgatcgt cgtgcagtgg ctgttcaaga gcctgtacga gatcgtcgcc
     2941 acgccgctga cgtacgtcgt cgtcggccgt ctgaagcgcg ccgaaggcgc tgacgtgttt
     3001 gaccgcgata ccaacttcag tccgttccat ctacagcagg tggatggttg agtggagttg
     3061 taccagggag ttgtgctggg atggaaaaga gtgagctgct gaaagacttg acactgctga
     3121 agcgcgacgc ggcgaagccg atgcgcaagt tggagacatt tccgaacctg caccccggtc
     3181 gcccgtacac gatcacgctg gagtcgaacg aattcacgtg cgtatgcccg gcgaccggac
     3241 agccggactt cgccacgatc accatacgct acgtgcccga tcagcgcatc gtcgaatcga
     3301 agtcgctcaa gctgtacctg tggtccttcc gcgacgaagg gcacttccac gagcacgtca
     3361 ccaacatcat cctcgacgat ctggtggcgg cgctcgaacc gcttcgctgc gaagtgatcg
     3421 gcaagttcaa cgtgcgcggc gggatcgcaa tcacggtcga ggcgaagtac gaacggccag
     3481 ttccttgagt tccttcagtt ccttattgaa tacgagaacg gaagaagctt aaggcactga
     3541 gggaactgaa gggagctcgt cgtgagctac gaagacgccc acaagctgct gcgtcgcaat
     3601 cgcgaattgt cgatcctcaa cgcgattgcc gaggcgctca accggtccgt cgacctgccg
     3661 caggcgctgg agggcgtgct gcggctcgtg gccgagctgc tcagcttgcg caccggctgg
     3721 atatggctgc tcgacgaaga caccggcgag ccgtacctgg ccgcggcgca gttcctgccg
     3781 cccgcgctgg cgaacgagcc ggagcgcatg accggctcgt gctattgcct ggacaccttc
     3841 gagcagggcg atctcaccgg cgcggccaat gtcaacgtcg tgacctgctc acgcctcaag
     3901 gcgctcatcg atggcaccga cgggctgcgc tatcatgcca gcataccgat ctattctcac
     3961 accgacaagc tgggcgtgct caacgtcgcc agcgccgact ggcgcgagct ggacgccgac
     4021 gacctgcgcc tgctgtacac gatcggctac cagcttggcg tggcgatcga gcgcgcccgt
     4081 ctctacgcgc gcagcgcgca gctcgccaag atcgaagagc gcaaccggct ggcgcgtgag
     4141 atccacgata cgctggcgca aggactgacg gcgatttccc tgcagctcga atcggccgac
     4201 gcgctgatcg acgcgtcacc cgcgcgcgcc aaggacaaac tgcgcaaggc gcttgagctt
     4261 gcccgcctga acctcgagga agcgcgccgc tcggtgctcg atttgcgcgc cgcgccgctg
     4321 ctcgaacggt cgctgcccga cgcgctgcgt gcgctggctg atagtatcgc acgtgagcgc
     4381 ggtctgtcga tcgacgtgca agtgcagggg gcggtcgatg ggatgccggg ccgcatcgaa
     4441 gcaggcgtct atcgcattgc ccaggaggcg ctgaataacg cggcgcggca ttccagcgcg
     4501 aaacgtatcg tcgtcagcct gagccgatcc gacgaccgcc tgcgcctggc cgtcgaagac
     4561 gacgggcgag ggttcgaacc gcaggcggcg acgaccggct tcggcctgat cggtatgcgt
     4621 gagcgggcgc gcctgctcgg cggcacgttg gaagtcgaca gcgccctgaa tcgcgggact
     4681 cgcatcctgc tggaaatacc tgtctgagtt ccttcatttc cttcagttcc tttagttcct
     4741 gcatattcac gggatgacac tcaaggaatt cagggaactc aaggaactca gggaacttag
     4801 ggaactcgtt ccatgatcac catctttctg gccgacgatc accccgtggt tcgcgatgga
     4861 ctgcgcgcta tcctcgagac gcagcctgac ttcgcggtga tcggcgaagc cggcgacggc
     4921 gcggaaaccg tctggcagac aaaggcgctt cgtcccgacg tgctcctgct cgacctggaa
     4981 atgccggaga tggacggcgt cgaagcgctg cggcacataa gtgaagccgc gcccgatacg
     5041 cgcgtgctgg tgttcacggc gtacgatagt gatgagcgca ttctggctgc cgtgcaggcc
     5101 ggtgtgcagg gctacctctt gaaaggcgcg ccgcgcgatc aggtgttcga cgcgatccgg
     5161 gtggtgcacg gcggcgggtc gctgctgcag ccggccgtcg cgtccaagct gctgaagcag
     5221 gtgcagagtg gaacagatcg caccgggagc cgggccggcg aattgacgtc gcgcgagatc
     5281 gaagtgctgc gcctgctggc gcagggacag gcgaataagc agatcgccgc gcggctgggc
     5341 attagcgagc gcacggtgaa attccacgtc agcgcgatct tccgcaagct cgacgcaacg
     5401 aatcgaaccg aagcggtcac cattgccgcc cagcgtggcc tcatcaaatt gtagcgtatt
     5461 gtctcacgaa ataggtgtat aatgatctta tctaggcaga cctctgctag gtttcgtcac
     5521 ggattgtcga ggtgtgcgct ccatcgggcc agattgactg accccagttg cgtaccccga
     5581 tcgatgtgtt gccgtttccg gcgtgggtgt catgtcgacg ctcgcacagg ttacgtctgc
     5641 cgtcagtttc ctgtccatgg cggcctcgct gtggctggga tgttatgtcg tcacgcgcag
     5701 cccgcgcagc cggctggcct ggcaggccgg gcttaccctg tggtcgctcg ccggcctctt
     5761 cgtcaccatt ctcctggcca tcggcgattc gacatcaccc tcaaggtggc ccaggtggcc
     5821 actgaccctc actctggcga tctggtatca cctgagcctc gaaaccctcc cgcctgaacg
     5881 gcgaaccact cagcgccggt ttctaccggt cgtgtacggc ctggctgccc tgcttgacgt
     5941 cttgctgctc cagacatcgc tgatcgcgag tgaccgtatg ccgggcgtcg gggcgtatac
     6001 gacggtgttt gaacgcaggc cgctgtttgc catatggccc attttcctta tcggcctcgc
     6061 actgctcatg ctgtacaact tctggacggc gcggcgggcc gtgcgcgatc ggatgcaccg
     6121 gaagcaactg aacagcctgg tgcgcggcac gtttctgggc gtgcttgccg ctgtctactg
     6181 gacggtggcc atcagcctgg gtgtgtccgt tcctgccctt ccgattgtcc tcgcgctggg
     6241 cctgggagtg ggcatgctcg gacatgggat cgcccgatac agcgcgctga tcgacggtcg
     6301 tattctgcga tttgatttcg acttgagcgc attgctcatg ttggcattgt cggccatcta
     6361 tttggcggcg acgtggttcc tgttccagcc tgcgtggtcg atcgccgccg caatcgtcgt
     6421 ggtcgcgctg acgatcttta cgcacacgag cttcgagttt gctcgacggc tgctggatcg
     6481 gccgtttgtg cggcttccgg cacgcgcctt gcgcgcgacg ctgcgccgag cggcaagcga
     6541 tctcggtgag cgcaaatccg tcgaggaaag ccttcgcagt gcacttgccg cgctcgtcgt
     6601 cggagtgaat gcccgctggg gcgcaatcgc gctgcgtgac ggcggcgaat ttgtcgtgcg
     6661 cgcctcgttt cacggagagc gggtgggcga gcgcctgccg gcccaccact tgaatgtgcg
     6721 cgagctgacc gttttgtcac ccgaaggctc gccggattgg cctctggccg tcattgcccc
     6781 gatgattgcc gaccgagatg cggtcggcgc aatcctgctc ggccagccga ggggcggcac
     6841 gcattacagt gaaggcgatc tcgacctggt ggccgaagcg gccgatcggc tggccgagtt
     6901 gatacggtat gcgcaggagc acgaagcgca tgcgagacag atcggcaaca tgctggaggc
     6961 atttcgagcg cgcgagtatg aactgcaaag tggcattgac ggactgcttt atcaggcgaa
     7021 ggcggcggcg cccagcccac aacaggttgc agaggtcgag aatgcaatgc ggcgcctgta
     7081 cgattactct tacctgggcg agcacccgct ggcgcggaat atgctcgcgc atgaccacgc
     7141 caccacgcac atcgatcgcg gcaaggcatt gaatgcggtg ctcgtcacag ccatcgaaaa
     7201 attacgcccg gccgcggttg agccgcgaga actgccacct cgtgagtggc atccttacct
     7261 gatcctgcgc gatgcctacc tgcgcggtga tgccaatcgt gacattatgt cgcgtctgta
     7321 cgtgagcgag gccaccttcc accgcacccg gcgcggcgcg ctgcgcgctg tgacgaaagc
     7381 tctccacgag atggatctca cccccgtcca ggcagactga cagtttttga caccttccca
     7441 tctggacttg acaaccgaat aggcgtctaa tgaggctcaa aggaggtgtg cgaatgagcc
     7501 acaagatgag tgtcacagcg ccaattcgcg gcgtcacgga tcgtgtgcga gcggcggtgg
     7561 atcgtttccg gcagcgaacc tcgtcgaacc gactcatcgc tgcgctgcgg cgggcgattc
     7621 cgagccaggg cctcctcagc gtgtggtggc gttctatcct caacctcgtt cgcctgctgc
     7681 gcaatctgct cacgttgttg gtgctggtcc cactgcgcgg gctttacat
//
LOCUS       DBNHXB010000414        13826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1297836, whole genome shotgun sequence.
ACCESSION   DBNHXB010000414 DBNHXB010000000
VERSION     DBNHXB010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 13826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13826
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1297836"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..649
                     /locus_tag="AC162G_16470"
     CDS             104..649
                     /locus_tag="AC162G_16470"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR5077452.1"
                     /translation="MSSRIGSQLPTHKSPSTGDPMAVDEQSMKRIAALCVSGVTVVTT
                     RHAEGLHGLTVSAFLAVSWDPPLTLISLESVNQSCDFIKESRIFAVSFLSDRQEFLAE
                     RFAGRAPLVNRRFDGVPHRFAVTGAPILEGALGWLDCRVEQIVEVGDHTLFIGRVVAL
                     GEGAPGNALAYYAHRYRTVEP"
     gene            884..1384
                     /locus_tag="AC162G_16475"
     CDS             884..1384
                     /locus_tag="AC162G_16475"
                     /inference="COORDINATES: protein motif:HMM:NF015957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR5077453.1"
                     /translation="MEKRLYRSRTDRMLGGVCGGLGAYFGVDPTLVRLVFALLFVFGG
                     SGFLLYLILWIVLPEEGRAYASSEETVRANTQEIAERGKQLGEDVKAAFGGASGTGAP
                     GALSAEARPAQTSNEGAKIFGLILIGIGVIFLLGNVLPAFSFSTWWPLVLVVVGVALL
                     VSQLRR"
     gene            1443..1676
                     /locus_tag="AC162G_16480"
     CDS             1443..1676
                     /locus_tag="AC162G_16480"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5077454.1"
                     /translation="MAKRRAKYEVEWNSERIRALRQHLGMTQRQMADELGMRQQTISE
                     WELGVYTPRRSTQKYLTLIAERARFTYTTKQQK"
     gene            1819..1992
                     /locus_tag="AC162G_16485"
     CDS             1819..1992
                     /locus_tag="AC162G_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077455.1"
                     /translation="MGTYSLDELVRRWFTHEITTEQAVGQLLQIVQEIERRVKELESK
                     SRYGGVPVGTPRG"
     gene            complement(2031..4382)
                     /gene="recD2"
                     /locus_tag="AC162G_16490"
     CDS             complement(2031..4382)
                     /gene="recD2"
                     /locus_tag="AC162G_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
                     /protein_id="HIR5077456.1"
                     /translation="MDTLEGIVERITYYNDENGYTVLRLKPHGRAGPLMVDQGLVTVV
                     GNLPEVTPGESLRLRGQWTTHRDHGRQFKAESCEQIRPATVEGIRKYLGSGLIKGVGP
                     VTAERIVKKFGLDTLDVLDHRPERIREVLGVGPKRAESIARAWEDQKVIKQVMLFLQS
                     HGVTTGLAVKIFKQYGDAAIDLVQSNPYQLASDIYGVGFKTADKIARQLGLPPDAPSR
                     VAAGVTYTLSQAADQGHVFLPQSALMREASSLLEVKPMQVEAVLGPLEEADQIRREVL
                     YPIVGSQRGEHGRRLEPAGDEHSEALMRTDEYPIVGERSPAPPSESPSIQALKEEPAI
                     YLTPFYHGEVGVARRLTALVESAASRLNELAGLDWPRVFGGLDAVAGKHATTRLSDKQ
                     REAVRMALSPHGKVTVLTGGPGTGKTTTVRAIITLLERYGKRYALASPTGRAAKRLSE
                     ATGRPAKTIHRLLEFAPAEGFKRGEQHPLDVDMLIVDEASMIDLLLMNNLLKAVPPGA
                     HVLLVGDVDQLPSVGAGDVLRDVIGSGRAAVVRLDVIFRQAQESLIIANAHRINKGQM
                     PYTPKDGRDFFHFVKEDPDGAAAMTIDVVTKRIPAKFGIDPWDIQVLAPMYRGAAGVN
                     ALNASLQQALNPPSPNTPERRLSGTLFRAGDRVMQTRNNYDKDVYNGDIGRVTGIDVE
                     NQLLRVTIDDRSIDYDWAEADELVLAYAVSVHKAQGSEYPAVVITMLTQHYLMLQRNL
                     LYTGVTRARRLCVVVGSRRALAIAVKNNKVAERYSGLQARLQR"
     gene            4560..6773
                     /locus_tag="AC162G_16495"
     CDS             4560..6773
                     /locus_tag="AC162G_16495"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5077457.1"
                     /translation="MQPSELIPNDADESVRYEGVERYRAFFETSNDALFVEDTAGRIL
                     DVNARACELLGYTRDEFRRISVADLLPFDTPHDPTDEEALQTQARFRLETVNRHKDGR
                     LIPVEVVGHRTSLGSQELWLVSVRDISDQKRLERALAGRSAQLARLNRLSKVLSKSLD
                     GAWIAQTAVNRIRALADLDAAAIAVVDDLRDAPTPIYYSSGISDAFKARFVAHTRTAD
                     RLIRQVATLGFPLIIDDVRNDTNLPSRIRRLVAAEGVRTLVLLPIVVGGKVRGVLAAA
                     YPRVTEVDEAILHLLEMAVGHVGHALANAWLFRREHQRAAQQQTLVDILSLAIQSMRT
                     EDLLSALAEHAGRLLGADGAFITRWDDVTHATVHVAAYGRYRDFYPSLPPTPADQPTL
                     TQAVLEAKRPIGVYDVQHTPFLRRSFAEKFSTRSLLGLPLIAHDRRLGALLIASDTPR
                     RFAPAEIELAQSAAHLVALAIDNARLHDELRQRAHSLSEEVSLRTQELRNANEQLRSL
                     DRLKSQLIEQIGHQFRTPVANIGTHLRLLESGRPEKRDHYMAVLNDQTEALKRLIETV
                     TAFAEVDLAPDARPYVPWPIANVVDPLTARYRLDAAAKSIQFDVVAGGAELRVRANPQ
                     RIGTALNEILSNAFDYTDEGGRVTLAVERVEEEGRGWVRISVIDTGVGIPADELPRVF
                     DQFFRGRQRLLQVRGVGIGLSLVQAIVQTEGGRVTAESAGVGQGSTFRLWLPEVR"
     gene            complement(6823..8121)
                     /gene="hisS"
                     /locus_tag="AC162G_16500"
     CDS             complement(6823..8121)
                     /gene="hisS"
                     /locus_tag="AC162G_16500"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR5077458.1"
                     /translation="MKSIIRPVKGTRDFYPEQMAFRNWLYGKMKEVSQRFGYQEYEAP
                     LLESLDLYAAKSGDELVKEQSYVFTDRGGDPITLRPELTPSLARMVAQKQRELPKPIR
                     WWSFGPFWRYERPQKGRTREFFQWNIDLIGAPSASAYADAEIVTVLAEFFRSVGLTPQ
                     YIAIKVSHRGLMEGQLNTLGIDLDKKDDVYRLIDKLDTLSPDEWAAYGRETIGLSAAQ
                     IDRLRDVLSDRDLWKQSSDLTTFFAAMTDLGASDYVEFDASIVRGLAYYTGTVFEAWD
                     RSGEFRAILGGGRYDNLVADVGGDPLGAVGFAMGDVVIGLLLEQYGLKPQLRSSPTRA
                     LVTVFSSELMGTSARVAATLRSAGINTELYPEPARLDRQLKYADTFGIPFAVIVGPDE
                     AAAGKATVKNLATGEQRQLDQAGVAEAVRPAGTGSVIGNR"
     gene            complement(8393..8584)
                     /locus_tag="AC162G_16505"
     CDS             complement(8393..8584)
                     /locus_tag="AC162G_16505"
                     /inference="COORDINATES: protein motif:HMM:NF016811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HIR5077459.1"
                     /translation="MISPTRQTVCGGLLEPDAPAIKAEYNGRTYLFCTRGCLRAFWSN
                     PEDFLAGKIEHPIDDDEGD"
     gene            complement(8761..12339)
                     /locus_tag="AC162G_16510"
     CDS             complement(8761..12339)
                     /locus_tag="AC162G_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077460.1"
                     /translation="MFSTRTPAFLVIVLLLAVTLAVVWGAASAGPTTPPRVAYIYDTD
                     TASRDSFDAMLTSRGFTVDLVPLGSAASYDFSPDEVIIVGNDTGTAGNWDAPDAIANV
                     DGAGKPIVGVGEGGSSFFDDINIFIDWGQSWSASGDSVYVVDPANLLWSTPIPISIPG
                     SNVVKLYTQASPYRAVDNPTLLSNVVRIGRQVDDAVHYPIISESVRTKCYALWGFYNS
                     PAAMTADGQDLFVNILLGRPCDAKANNTPTPTSQLPACQELVDPFKLPSPALINFDDL
                     THGQVIGTAYQAAYGVTFENSPTTRAIIYGNEPAEAQSKPNVATNDAVFPETSNDVPM
                     NLGFSSPKTHVGFYIGNGEFISPTAMLTAYDAFDHIICQVRNTPVPEPHTEFIGVYDP
                     TGRIVRISLDYGNTSLSESIDDLYFAPAPGGPGPAPTRTPMPTWTPIPSPTPTSGPTA
                     TPMPVIPVYPYVPPVIIAPIIPPVGDLSIHGIEITQGIQCFNPAQGLASCPDNSLPLV
                     AKKDATARIYLKLTGGASSSLSNVPVRLHIFANGVEYIGNASGKATGSINQANDDSAD
                     IYFTVNFSNDVLVSFYAVVDESNVIAETNESNNRYPASGTISLNFRKRDTLKIVGQRV
                     RYHPSGYSGTQYAGGWAVNGGAADWWEQMLPIRNNGINYSVASGYLNWTKMLTPCSSD
                     TGSNNQHDLIQKLNTQWFLQNVFSWLFGTTFTGADHVYGWVPDAGYPCGHADMPVYPH
                     AGGFGVVGIGTDAPGASTDNPGAGALIFGHELTHDYDVKHTDTGADDCGSNDSSSDFP
                     YGTSSIQEFGFNPITGKIYNPASTHDLMSYCPAGGSKEGWISPFTWNRMSSDLDALAL
                     LAASSAQAGRPYAFNVTSSSESLVVDATIFNPDVVGTVSGTLGSLHRVSAGLAYPLPA
                     GNYAVELRSGATALISQTFSVSFESEYSAHSGPNSDPPTTGITPTTQADITFVMPWAD
                     GATSVVLLYNNQVLDSRAVSANAPNVTITSPTQVVTWTTGTQTVAWTASDLDGDPLTY
                     SVLYSHDAGASWELLAEGLTNPSFSVDADSLAGGSDARIRVVATDGVNIGYDETDAPI
                     VVPDKAPYALITNPVSGQIIAPGNLLVLQGTGTDLEDGGLPDSALHWSSSRDGSLGDG
                     PSLGLTSLSRGPHVITLTVMDSHGQTTSVSVSIFVGHGVYLPVVLK"
     gene            complement(12445..>13826)
                     /locus_tag="AC162G_16515"
     CDS             complement(12445..>13826)
                     /locus_tag="AC162G_16515"
                     /EC_number="4.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016841 - ammonia-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0170035 - L-amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAL/PAL/TAL family ammonia-lyase"
                     /protein_id="HIR5077461.1"
                     /translation="NTGFGVFASRQVAREDAERLSRNLILSHTVGVGEPLPEDVVRAA
                     MLIRANTLAIGHSGVRPEIIDTLVAMLNRRVTPVVPEKGSLGASGDLAPLSHMALVLT
                     TDAADRDEDSGEAFFEGRRMTGKAAMRRAGIPRLILGAKEGLALNNGATFSAAQAALA
                     VHDAGVLLDNALVAAAMSFEALRGVSRALDERLHRVRRQAGQIEIAARLRELIAGSTL
                     VDSTDRVQDAYSLRAIPQVLGPIKETIDFVRGIVERDINAATDNPLIFLDLPSEDKAL
                     SGANFHGEPIALAMDYLKIAVSDVASMSERRLFRLTDEKLSDGLPAMLVEHGGLNSGF
                     MMAQYTAAALVSDNKTLAHPDAVDSIPTSASQEDFNPMSMNAARRCAEIIDNAQHVIA
                     LEMLAAAQALDLRMRADPNARLGRGTRRALDTIRLKTTFLEFDRLLAPEVATIVGMVQ
                     NGSIARAAA"
BASE COUNT         2389 a         4251 c         4578 g         2608 t
ORIGIN      
        1 caaacggacc tccgcccgcg acccatccac tatactctgg cagcttggac atggtagacg
       61 gccaggggca cgatagatgt tggggcgcgg atgagtcaaa gctatgtctt ccaggattgg
      121 gagccagtta ccgacgcaca agtcgccatc aaccggcgat ccgatggccg ttgacgagca
      181 atccatgaag cgtatcgcgg cgctgtgcgt ctccggcgtg acggtcgtca cgacgcgcca
      241 tgcggaaggc ctgcacggcc tcaccgtcag cgcctttctg gcggtctcgt gggatccgcc
      301 cctcacactg atttcgctcg agagcgtgaa ccaaagctgt gatttcatca aagagagcag
      361 aatcttcgcc gtgagctttc tgagcgacag gcaggagttc ctggccgaac ggttcgccgg
      421 ccgcgccccg ctggtcaatc ggcgcttcga cggcgtgccg catcgtttcg cggtgaccgg
      481 cgcgccgatc ctcgaaggtg cgctgggctg gctggattgc cgtgtcgagc agatcgtcga
      541 ggttggcgat catacgttgt tcattgggcg cgtcgtggcg ctcggcgaag gcgcgccggg
      601 caacgcgctg gcctattatg cgcatcgtta ccggacggtc gagccgtgaa cggcgtgcgg
      661 ggccgactga acaagcgttc ggagacggac tacatcgaaa gactgccgcg caaatcggtc
      721 tcgaggcggt tggccgcgct tgtaggctcg cctatactgt ggccgaattg atcatcgtat
      781 taggagcaat gctgtttagt tagcgggcca ggcccccacg cccggcggca tgggagccgc
      841 ctccgctacc ttatctaaac agtattggca ttaggaggca atcgtggaaa agcgactcta
      901 tcgtagccga accgaccgga tgctgggggg tgtatgtggc ggcttgggcg cctacttcgg
      961 agtcgatccg accctggtta ggctcgtgtt cgcgctgttg ttcgtcttcg gcggatcggg
     1021 tttcctgctg tacttgatct tatggatcgt cttgccggag gagggtaggg cgtacgcttc
     1081 atcagaggag acggtccgcg cgaacactca ggagatcgcc gagcgcggca agcaattggg
     1141 cgaagacgtg aaggcggcct tcgggggtgc atccgggacg ggcgccccag gtgcattgtc
     1201 ggcggaggcg cgaccggcgc aaacctcgaa cgagggggcc aagatcttcg gattgatcct
     1261 gatcgggatc ggcgtgatat tcctgctggg taacgtgctg ccggccttct ccttctcgac
     1321 gtggtggccg ctcgtcctgg tcgtcgtcgg cgtggcgctg ctggtcagcc agttgcgtag
     1381 gtgacacatt gggcagttgc gcgcggcgtt tcggcgtgct aaactccggc caggagtgag
     1441 gcatggccaa gcgccgcgcg aagtacgagg tcgagtggaa ctcggagcga atcagggcct
     1501 tgcgccagca tctggggatg acccagcggc agatggcaga cgagctgggc atgcggcagc
     1561 agacgatcag cgagtgggag cttggcgtgt acactccccg ccgatcgacc cagaagtacc
     1621 tgacgctcat cgccgagcgc gcccgattca catacacgac gaagcagcag aagtgaaggc
     1681 agaggacgaa cgagggcttt caccaaaatt gggccttgac aaatacacgg tatcgtgtta
     1741 aactgtacgg cgcacgcagc agacggctgc gcttatttta ccttagcgat acacgctaac
     1801 gtgtagaggg aggcgacgat gggaacctat tcgctggacg agctggtgcg ccggtggttc
     1861 acgcacgaga ttacgaccga gcaggcggtc gggcaattgc tgcagatcgt tcaggagatc
     1921 gagcggcggg tgaaagagct cgaaagcaag agccggtacg gcggcgtgcc ggtcggcacg
     1981 ccgagggggt gacagtagga ggcatctcca gatgccgatc cagatgccga tcagcgctgc
     2041 aggcgcgcct gcaatcccga ataacgctcg gcgaccttgt tgttcttgac cgctattgcc
     2101 agcgcgcggc ggctgccgac gactacgcac agccgcctgg cgcgggtgac gcccgtatac
     2161 agcaggttgc gctgcagcat gaggtagtgc tgggtgagca tcgtgatcac gacggcgggg
     2221 tattcgctgc cttgcgcctt gtgcaccgac acggcgtacg ccagcacaag ctcgtcggct
     2281 tcggcccagt cgtagtcgat cgagcggtcg tcgatcgtca cacgcagcag ttgattctcg
     2341 acgtcgatgc cggttacgcg tccgatgtcg ccgttgtaca cgtctttgtc gtagttgttg
     2401 cgcgtctgca tcacgcggtc gccagcgcgg aacagcgtgc cgctcaggcg tcgctccggc
     2461 gtgttgggcg atggggggtt gagcgcctgc tgcaggctgg cgttcagcgc attgacgccc
     2521 gccgcgccac ggtacatcgg cgcgagcacc tggatgtccc aggggtcgat gccgaacttg
     2581 gccgggatgc gcttcgtcac gacgtcgatc gtcatcgccg ccgctccgtc ggggtcttcc
     2641 ttgacgaagt ggaagaaatc gcgcccgtcc ttcggcgtgt aaggcatctg ccccttgttg
     2701 atacgatgcg cgttggcgat gatcagcgat tcctgcgcct ggcggaagat cacgtccagg
     2761 cggacgacgg cggcgcggcc tgacccgatc acgtcgcgca gcacgtcgcc cgcgccgacg
     2821 cttggcaatt gatcgacgtc gcccacgaga agcacgtgcg caccgggtgg caccgccttc
     2881 aacaggttgt tcatcagcag taagtcgatc atgctggctt cgtcgacgat tagcatatcg
     2941 acgtcgagcg ggtgctgctc gccgcgcttg aacccctcgg cgggcgcgaa ctccagcagg
     3001 cggtgaatcg ttttcgccgg gcgacccgtc gcttcgctca gacgcttggc ggcgcggccc
     3061 gtcggcgagg caagcgcgta gcgcttgccg tagcgctcca gaagtgtgat gatcgcgcgc
     3121 acggtggtcg tcttcccggt gcctgggccg cccgtcaaaa cggtgacctt gccgtgtggt
     3181 gacaatgcca tcctcaccgc ctcacgctgt ttatccgaca gtcgtgtcgt ggcatgcttt
     3241 ccggcgacgg cgtcgaggcc gccgaacacg cgcggccaat cgaggccggc caactcgttc
     3301 agccgcgatg ccgccgattc gaccagcgcc gtcaggcggc gcgcaacgcc gacttcgccg
     3361 tgatagaatg gcgtcaggta aatggcaggc tcttccttga gcgcctgaat gctcggtgat
     3421 tcagatggcg gcgccggtga gcgctcgcca acgattgggt actcgtccgt ccgcatgagg
     3481 gcctcggagt gttcgtctcc ggccggctcg agccggcgtc cgtgttcacc acgctgcgag
     3541 ccgacgatcg gatagagaac ctcgcgcctg atctgatcgg cttcctccaa aggtcccagc
     3601 acggcttcga cctgcatggg cttgacctcg agcaaagatg acgcttcgcg catcagcgcc
     3661 gactgaggca ggaacacgtg tccctggtcg gcggcttggc tgagggtgta cgtgacgcct
     3721 gcggcgacgc gcgacggcgc gtcaggcggc aagccgagct ggcgcgcgat cttgtcggca
     3781 gtcttgaatc ctacgccgta gatgtcgctc gccagttgat atgggttgct ttgcaccaga
     3841 tcgatcgccg catcgccgta ctgcttgaag atcttgacag ccagaccggt cgtcacgccg
     3901 tgggactgca ggaagagcat cacttgcttg atgaccttct ggtcttccca tgcccgggcg
     3961 atcgactcgg cgcgcttcgg cccgacgccc agcacctccc gtatgcgctc cggccgatga
     4021 tcgagcacgt caagcgtgtc gaggccgaat ttcttgacga tgcgctccgc cgtcaccggc
     4081 ccgacaccct taatcaagcc tgagccgaga tacttcctga tgccctcgac ggtcgccggc
     4141 ctaatctgct cgcaggattc ggccttgaac tgccggccgt ggtcgcggtg cgtcgtccat
     4201 tggccgcgca atctcagcga ctcgccggga gtgacctccg gcaggttgcc caccaccgtg
     4261 acaagcccct ggtcgaccat gagcggaccc gcgcgcccgt gtggcttgag gcgcagcacc
     4321 gtgtagccgt tttcgtcgtt gtaatatgtg attctttcga cgatgccctc gagcgtgtcc
     4381 atcgtggaca atcatagcac gggcgcgcgg ggattcaaac tggttgcgaa cggcgagatc
     4441 gcgtaaaatg gcgtgtgcgt gccctgcggc aagccgatgg atgccttcgg cgataaggcg
     4501 cagccaggag acgaggcagc aatgggatac cgtcatatgt tcatctcagg cgagttgtga
     4561 tgcagccgtc cgagcttatt cctaatgatg ctgacgaatc cgtcaggtac gaaggggtcg
     4621 aacgctaccg ggcgttcttt gagacgtcca acgatgcctt gttcgtcgag gacacagccg
     4681 gccggattct cgatgtcaat gcgcgtgcct gcgagctgct cggctacacc cgcgatgagt
     4741 tccgacgcat atcggtagcc gatctgctgc ctttcgacac gccgcatgac ccgaccgacg
     4801 aggaagctct tcagactcag gcgcgctttc gtctggagac ggtcaatcgt cacaaagatg
     4861 ggcgtctgat tcccgtcgag gttgtcggac accgtacctc actgggtagc caggagctgt
     4921 ggctcgtcag cgtgcgcgac atcagcgacc agaaacggct ggagcgcgcc ttggccggac
     4981 gcagcgctca actggcgcgc ctcaaccgcc tgtcgaaggt gctgtcgaag tcgctcgacg
     5041 gcgcctggat cgcccagacg gcggtaaacc ggatccgggc actcgccgat ctggatgcgg
     5101 ccgcgattgc cgtcgtagac gacctgcgcg atgcgccgac gcccatctat tactcctcgg
     5161 gcatatcgga tgccttcaag gcgcgtttcg tcgcacacac gcgtacggcc gatagactga
     5221 tccggcaggt cgctaccctc ggctttccgc tcattattga cgacgtgcga aacgatacga
     5281 acctgccgtc gcgcatacgg cgactggtcg ccgccgaggg tgtgcgcacg cttgtcttgc
     5341 ttcccatcgt cgtcggcgga aaggtgcgcg gcgtgctggc agccgcttac ccgcgcgtca
     5401 cggaggtgga cgaagctatc cttcacttac tggaaatggc ggttgggcac gtggggcatg
     5461 cgctggcgaa cgcctggctg tttcggcgtg aacatcaacg cgcagcgcaa cagcagacgt
     5521 tggtcgacat tttgagcctt gcgattcaat cgatgcgcac ggaggacttg ctgtcggctc
     5581 tggccgaaca cgccggccgc ctgctcggcg ccgacggcgc gttcatcacg cggtgggacg
     5641 acgtcacaca tgccacggtg catgtcgctg cctatggtcg ttatcgcgac ttctacccct
     5701 cgctgccacc tacgcccgca gaccaaccga ctctcacgca ggcggtgctg gaagccaaac
     5761 gcccgattgg cgtgtacgat gtgcaacaca cgcctttcct gcgccggagc ttcgccgaga
     5821 agttctcgac gcgttcgctg ctcggtctgc cgttaatcgc ccatgaccgc cgccttggcg
     5881 cgctcctgat cgcatcggac acgccgcgcc gattcgcgcc ggccgagatc gagcttgctc
     5941 agagcgcggc ccacctcgtc gcgctggcga tcgacaatgc gcggctgcac gacgaattac
     6001 gccagcgggc gcacagcctg tccgaagaag tgtctctgcg cacgcaagag cttcgcaacg
     6061 ccaacgagca actgcgatcg ctcgatcggc tcaagagcca gttgatcgag cagatcgggc
     6121 atcagttccg cacgccggtg gcgaacatcg gcacgcacct gcgcctgctg gaatccggca
     6181 ggccggaaaa gcgcgatcac tacatggccg tcttgaacga tcagacagag gcgctgaagc
     6241 gcttgatcga gaccgtcacc gcgttcgccg aagtcgacct ggcgcctgac gcgcgccctt
     6301 acgtgccgtg gccgattgcg aacgtcgtcg atcctctgac ggcgcgctat cgtctggacg
     6361 cggccgccaa gtcgatccag ttcgacgtcg tcgcaggcgg cgccgaacta cgcgtgcgag
     6421 ccaacccgca gcgcatcggc acggcgctca acgaaatcct gtccaatgcg ttcgactaca
     6481 cggatgaagg cggccgtgtc acgctggcgg tcgaacgagt cgaagaagag ggacgaggct
     6541 gggtgcgcat ctcggtgatc gacaccggcg tcggcattcc cgccgacgaa ttgccgcgcg
     6601 tattcgacca gttcttccgg ggtcgccagc gattgctgca ggtgcgcggc gtcggcatcg
     6661 gcctgagcct ggtgcaggct atcgtccaga cggaaggggg acgtgtcacg gccgaaagcg
     6721 caggtgtggg gcagggcagc actttccggc tgtggctgcc ggaggtaagg tgatcggtaa
     6781 ttggtaatta gtgatcagtg agcagcaatc aatgaccagc aattaccgat taccaattac
     6841 cgatccagtt ccagcggggc gcaccgcctc ggccacgccc gcctgatcca actgccgctg
     6901 ctcgccggtc gccaggttct tcaccgtcgc cttgcccgcc gccgcttcgt ccggcccgac
     6961 gatcacggcg aaggggatgc cgaaggtatc ggcgtatttg agctgccggt cgagcctggc
     7021 cggttcggga tatagctccg tgttgatacc ggccgaacgc aaggtggcgg cgacgcgcgc
     7081 cgaagttccc atcagttccg atgagaacac ggtcaccagg gcacgcgtcg gcgaggagcg
     7141 cagttggggc ttgaggccgt actgctccag cagcagcccg atcaccacgt cccccatcgc
     7201 gaagccgacc gcgcccaagg gatcgccgcc tacgtcggcg acgagattgt cgtagcgacc
     7261 gccgccgagg atggcgcgaa actcgccgct gcgatcccac gcctcgaaca ccgtacccgt
     7321 gtaatatgcg agcccgcgaa cgatcgacgc gtcgaactca acgtagtcgc tcgcgccgag
     7381 gtctgtcatt gccgcgaaga aggtcgtcag gtcgctcgat tgcttccaca ggtcgcggtc
     7441 gctgagcaca tcgcgcagtc gatcgatctg cgccgcgctc aacccgatcg tctcccggcc
     7501 gtaggctgcc cactcgtccg gcgacagcgt atccagcttg tcgatcaggc ggtacacgtc
     7561 gtccttcttg tccagatcga taccgagcgt attgagctgc ccctccatca ggccgcgatg
     7621 gctcactttg atggcgatat attgcggcgt gaggccgacc gagcggaaga actcggccag
     7681 gacggtgacg atctcggcgt cagcgtacgc ggatgcggaa ggcgcgccga tcaggtcaat
     7741 gttccactgg aagaactcgc gtgtccgccc tttctgcggg cgctcgtagc gccagaacgg
     7801 gccgaacgac caccagcgga tcggcttggg cagctcgcgc tgtttctgtg cgaccatgcg
     7861 cgccaggctg ggcgtcagct cggggcgcag cgtgatcggg tcgccgccgc ggtcggtgaa
     7921 gacgtaggac tgctccttga ccagctcatc gccggatttg gcggcataca ggtcgagcga
     7981 ctcgaggagc ggcgcttcgt attcctgata gccgaaccgc tgcgagactt ccttcatctt
     8041 gccatatagc cagttgcgga aggccatctg ctccgggtag aagtcgcggg tgcctttgac
     8101 ggggcggata attgatttca tggcgtcttc tccaattgtt gcgaatcggc gagtcagcaa
     8161 gaccggcgtc gaagttcctg gaactgaggg aacgaaggga actaaagcaa aaagaaaagg
     8221 cgcggctcgc tgcgcgtggg ccacgccttt ctacagtcga acggaacgtc gctcagacgc
     8281 tacccaaacg ccaatagccc acgtgagtgt gggcatgatt ggtgtgaaga tgcgtgaggg
     8341 aaagcgtctg ccgtttcata caattcatat tataccatgc gtcgagtgac tgtcaatcgc
     8401 cttcgtcgtc atcgatcgga tgctcaatct ttccggcgag gaaatcctcg gggttgctcc
     8461 agaacgcgcg caggcagccg cgcgtgcaga agaggtaggt ccggccgtta tattcggctt
     8521 tgatggcagg cgcatccggc tcaagcagac cgccgcacac agtttggcga gtaggtgaaa
     8581 tcacttctga ctcctgagga agaggcccgc cggctacccg gcgggcctct tcaatcggat
     8641 cagccgcgct catagatcac tttgccttcg cgcaaggctg gccgtagcac gtcaccgacg
     8701 aggttgcccc ggcgctcgat ctcgtcgggc gtgtcacgat gatgtctttt ccgagttgtc
     8761 ttacttcaac acgaccggca ggtagacacc gtgacccacg aagatcgaaa cggacacact
     8821 ggttgtctgg ccgtggctgt ccatcacggt gagcgtgatg acgtgcgggc cacgagacag
     8881 cgaggtcagt cccagcgacg ggccatcgcc caggctgccg tcgcggctgc tcgaccagtg
     8941 cagcgccgaa tcgggcagac cgccgtcttc gaggtcggtc cccgtgccct gaagcaccag
     9001 caggttgccg ggcgcgatga tctgcccact gaccgggttg gtgatcagcg cgtagggcgc
     9061 cttatccggc acgacgatcg gagcatcggt ctcgtcgtag ccgatgttga cgccgtcggt
     9121 tgccacgacc cggatgcgtg cgtcgctccc gccagccaac gagtcggcat ccacggagaa
     9181 ggatgggttg gtaagccctt cggcgagcag ctcccagctc gcgccggcgt cgtgactgta
     9241 cagcacagag taggtgagcg ggtcgccatc cagatcgctc gccgtccacg cgaccgtttg
     9301 tgtcccggtt gtccaggtga cgacttgggt cgggctggtg atggtgacgt tcggcgcgtt
     9361 cgcgctgacg gcgcgcgaat cgagcacctg gttgttgtaa agcagcacga ccgaggtcgc
     9421 gccgtccgcc cacggcatga cgaatgtgat gtcagcctgc gtggtgggcg tgatgccggt
     9481 ggtaggcggg tcgctattgg ggccgctgtg cgcgctgtac tcgctctcga agctgaccga
     9541 gaaagtctgg ctgatcagcg cggtagcgcc actgcgcagc tcgaccgcat agttgccggc
     9601 gggcagcggg tacgccaggc cggcgctcac gcgatgcagg ctgccgagcg tgccgctcac
     9661 ggtgccgacg acgtcggggt tgaagatagt cgcgtcgacg acgagcgact cgctgctcga
     9721 ggtgacgttg aaggcgtagg ggcgccctgc ctgcgcgctg ctggctgcca ggagtgccag
     9781 cgcgtcaagg tcgctgctca tgcggttcca ggtgaagggc gagatccagc cttccttcga
     9841 gccgcctgcc ggacagtagc tcatcaggtc gtgtgtgctg gcggggttgt atatcttgcc
     9901 ggtgatcggg ttgaagccga attcctgaat gctcgaggtg ccgtacggaa agtccgacga
     9961 gctgtcgttc gagccgcaat cgtccgcgcc ggtgtcggtg tgcttgacgt cgtagtcgtg
    10021 tgtcagctcg tggccgaaga tgagcgcgcc cgcgcccgga ttgtcggtgc tcgcgccggg
    10081 cgcatcggtg ccgatgccga cgacgccgaa cccgcccgcg tgcggataca ccggcatgtc
    10141 ggcgtggccg cacggataac cggcgtctgg cacccagccg tagacgtgat ccgcacccgt
    10201 gaaggtggtg ccgaagagcc agctaaagac gttttgaaga aaccactgcg tgttgagctt
    10261 ctggatcagg tcgtgctggt tgttcgaccc cgtatcgctg ctgcacggcg tgagcatctt
    10321 ggtccagttc aggtagccgc tcgcgacgga gtagttgatg ccattattgc ggatgggcag
    10381 catctgctcc caccagtcgg ccgcgccgcc attcaccgcc cagccgccgg cgtactgcgt
    10441 gccgctgtag ccggatggat ggtagcgcac ccgctggccg acgatcttga gtgtgtcacg
    10501 cttgcggaag ttgagcgaga tcgtgccgct ggcgggatag cggttgttgc tttcgttcgt
    10561 ttccgcgatg acattgctct cgtcgacgac ggcgtagaaa ctgaccaaca cgtcattgct
    10621 gaagttgacg gtgaagtaaa tgtcggcgct gtcgtcgttg gcctggttga tgctgccggt
    10681 cgccttgccg gaagcattgc cgatgtactc gacaccgttg gcgaagatgt gcagtcgcac
    10741 cggcacgttg ctcaggctcg aagacgcgcc gccggtgagc ttgaggtaaa ttcgcgccgt
    10801 ggcatctttc ttggcgacga gcggcagcga gttgtccggg caactggcca gcccctgcgc
    10861 cgggttgaag cattggatgc cctgggtgat ctcgatgccg tggatcgaca ggtcgccgac
    10921 aggcgggatg atcggcgcga tgattacggg cggaacgtat gggtacaccg ggatcaccgg
    10981 catcggcgta gccgtcgggc cactcgtcgg cgtcggcgaa gggatcggcg tccacgtcgg
    11041 catgggtgtg cgcgtcgggg ccgggccggg accgccgggc gcgggcgcga agtacagatc
    11101 gtcgatcgat tcggacagag acgtgttgcc gtagtccagc gaaatgcgga cgatccgtcc
    11161 ggtcggatcg tacacgccga tgaattccgt gtgcggctcg ggcaccggcg tgttgcgcac
    11221 ctggcagatg atgtggtcga acgcatcgta ggccgtcagc atcgccgtcg gcgagatgaa
    11281 ctcgccgttg ccgatataga agcccacgtg cgtcttgggt gaactgaagc cgaggttcat
    11341 tggcacgtcg ttgctggttt cagggaagac ggcgtcgttg gtggcgacgt tcggctttga
    11401 ctgcgcttcg gccggctcgt tgccgtagat gatcgcccgc gtcgttgggc tgttctcgaa
    11461 cgtcactccg tacgcggcct ggtaagccgt gccgataacc tggccgtggg tcaggtcgtc
    11521 gaagttgatg agcgccggcg acgggagttt gaacggatcg accagctcct ggcacgccgg
    11581 caactggctc gtgggcgtgg gcgtgttgtt cgcctttgcg tcgcacggcc ggccgagcag
    11641 aatattgacg aagaggtcct ggccgtcggc ggtcatcgcc gccggcgagt tgtagaagcc
    11701 ccacagcgcg tagcacttgg tccgcacgct ctccgagatg atcggataat gaacggcgtc
    11761 gtctacctgc cggccgatgc ggacgacgtt cgacagcagc gtcgggttgt cgaccgcccg
    11821 ataaggagac gcctgcgtgt acagcttgac gacgttactg cccggtatcg agatagggat
    11881 gggcgtcgac catagcagat tcgccggatc gacgacgtag acgctgtcac ccgatgcgct
    11941 ccaactctgc ccccagtcga tgaagatatt aatgtcgtcg aagaacgagc tgccgccctc
    12001 gccgacgccg acgatgggct tgcccgcgcc gtcgacgttc gcgatcgcgt cgggcgcgtc
    12061 ccagttgccc gccgtgcccg tatcgttgcc gacgatgatg acttcatccg gcgagaagtc
    12121 atacgaggca gcgctgccaa gcggaaccag atcgacggtg aagccccggc tggtgagcat
    12181 cgcgtcgaag ctgtcccggc tggccgtgtc ggtgtcgtag atgtaggcca cgcgcggcgg
    12241 cgtagtcggg cctgctgatg cggcgcccca aacaacggct agagttacgg cgagaaggag
    12301 aacgatgacg aggaatgcag gcgtgcgggt cgagaacatg gtagcctccc ttggctaaag
    12361 tggctgcgat gcgtcgatgc tacgaccgag ttgcaggaaa caggataagc ccgttaccga
    12421 accggcggca atagaccatt ggcctcatgc cgccgcccgg gcgatcgatc cattttgcac
    12481 catccccacg atggttgcca cttccggtgc gagcaagcgg tcaaactcca ggaacgttgt
    12541 cttcagccga atggtgtcca acgccctccg cgttccccgg cccagccgcg cattcggatc
    12601 ggctcgcatc cgtaggtcga gcgcctgcgc cgccgccagc atctccagcg cgatcacgtg
    12661 ctgcgcgttg tcgatgatct ctgcacagcg gcgggccgcg ttcatcgaca tcggattgaa
    12721 gtcctcttga ctggccgaag tcggaatcga gtcgaccgcg tcgggatgcg ccagcgtctt
    12781 gttatcggac acgagcgcgg ccgccgtgta ctgcgccatc atgaagccgc tgttcaggcc
    12841 gccgtgctcg acgagcatgg cgggcaggcc gtcgctcagc ttttcgtcgg tcaggcggaa
    12901 caggcggcgc tcgctcatgc tggccacgtc ggatacggcg atcttgaggt aatccatcgc
    12961 cagcgcgatc ggctcgccgt ggaagttcgc accggacagc gccttgtcct cggatggcag
    13021 gtcgaggaag atcagggggt tgtcggtcgc cgcgttgatg tcgcgctcga cgatgccacg
    13081 cacgaagtcg atggtctctt tgatggggcc gagcacctgc ggaatggcgc gcagcgagta
    13141 ggcatcctgg acgcggtcgg tcgaatcgac gagcgtgctg cccgcgatca gttcgcgcag
    13201 gcgcgcggcg atctcgatct gcccggcctg ccggcgtacc cggtgcagcc gttcgtcgag
    13261 cgcgcgcgac acgccgcgca gcgcctcgaa cgacatcgcc gcggcgacga gcgcgttatc
    13321 cagcagcacc ccggcgtcgt ggacggccag tgcggcctgg gcggcgctga acgtcgcgcc
    13381 attgttgagc gcgagtcctt ccttcgcgcc caggatcagt cgcggaatgc cggcgcgccg
    13441 catcgccgct ttgcccgtca tgcggcgccc ttcaaaaaag gcttcgccgc tgtcctcgtc
    13501 gcggtcggcg gcgtcggtcg tcagcacgag cgccatgtgc gacagcggcg ccagatcgcc
    13561 gctcgcgccg agcgatccct tctccggcac gaccggcgtg acccgcctgt tgagcatcgc
    13621 gaccagcgta tcgatgatct ccggccgcac gcccgaatgg ccgatggcca gtgtgttggc
    13681 gcggatcagc atcgcggcgc gaacgacgtc ttcgggcagc ggctccccga cgcccacggt
    13741 gtggctgagg atcaggttcc gtgacaggcg ttccgcgtct tcgcgcgcga cttggcggct
    13801 ggcaaatacg ccgaagccgg tgttga
//
LOCUS       DBNHXB010000415         3375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4653519, whole genome shotgun sequence.
ACCESSION   DBNHXB010000415 DBNHXB010000000
VERSION     DBNHXB010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4653519"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..405
                     /locus_tag="AC162G_16520"
     CDS             235..405
                     /locus_tag="AC162G_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077462.1"
                     /translation="MQVRYRLFKSVMKSWEDLCADAAAFASTIGRERLINISVSQADT
                     GGQGVIFVWYWE"
     gene            complement(541..1518)
                     /locus_tag="AC162G_16525"
     CDS             complement(541..1518)
                     /locus_tag="AC162G_16525"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5077463.1"
                     /translation="MSYVRGVFVTTLATLTFVTLLITATSLGAQAGTQAAAQAPLTVQ
                     DGVYSEAQARRGQALYAQRCAGCHGPSLAGASAPALAGDLFTNKFRMEPLSALFIQIR
                     YAMPPRAEANAQLTSEQAADLVAYVLKSNGFPAGQTDFPAAGATNSPIGWPTGRGVGA
                     SSAPSPSWYSPTGNLAQLMRGVFFHNSNLVFSIQEVEAKDLPPQPATRPGGSTTFDWG
                     LMVYTGWPAVENATAALADASALLMLPGLRCENGRAAPITEPDWIKFTDQMISVARRS
                     YRLAQTKNKEALSEFTLDLTNACSACHRVYRDVGGRGGAGNTSGRCMHR"
     gene            complement(1599..2603)
                     /locus_tag="AC162G_16530"
     CDS             complement(1599..2603)
                     /locus_tag="AC162G_16530"
                     /inference="COORDINATES: protein motif:HMM:NF052887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8021 domain-containing protein"
                     /protein_id="HIR5077464.1"
                     /translation="MHSARYLMHSARFLLIAAFATGIASAQTCDRACLKTTLDQYLNA
                     VVKHDTSAAPLFVGFRQTDNGVVVRPGTGTWQSITALGDIQRKYFDPVSGQAAYLGLI
                     KEGQATDIATLRLRVENRQITEAEWVIAREGAIGPSGTAGGNLYNLAGFIAEPPPDRT
                     VPQNQRASREVLIAIANSYFDGLTTHDGSIIMAHEGCTRNENGTHTAGPNPNAGAAAR
                     GGPGGRGDCTSNLTNFNVSLISARRYPVVDVEQQAVVGFGIFLRKPGTTLMRNLVAEW
                     FFVDNNKIRNIWASMFYPTNDLPVPNWPPYEGNFPVAAEFAAPAAPAAPGPGRGAGRQ
                     "
BASE COUNT          648 a         1026 c         1023 g          678 t
ORIGIN      
        1 accggcttcc cgtcacttga ccgtttatgt tcgcagggga aatcaaatga atatggaatc
       61 ttataatctg ccgctcagga actggcaatt tgcgggttac acagatgcag cccggcctga
      121 cggcgcgcct ccctggggat gaccacgccc ggcagaatcg ttggtagaaa tgatggcaat
      181 catttgccat cattgggcct cttcggtgag gaagttgaga agggggagcg aacgatgcag
      241 gttcgttaca ggttgttcaa gagcgtgatg aagtcgtggg aggacctgtg tgctgacgcg
      301 gcggctttcg cctcgacgat cgggcgcgag cgcctgatca acatctcggt ctcacaggca
      361 gacaccggcg gccagggcgt gattttcgtg tggtactggg aatgagtacg taggcggccc
      421 tttcagtgcc gcgcccgttg cgtctaccgg tcagggacac ctccggcgtc gtcggaaatc
      481 ttcgtagcag cgtcgactgc cttcgcgctc ttcgccagtt ccttcttcgc gcattcgtgg
      541 ttatcggtgc atgcagcgcc cagacgtgtt tcctgcaccg ccgcggccgc cgacgtcgcg
      601 atacacccgg tggcatgcac tgcaggcgtt cgtcagatca agcgtgaact ccgagagggc
      661 ttccttgttc ttcgtttgcg ctagccgata cgaccgcctg gccacggaga tcatctgatc
      721 ggtgaacttg atccagtcag gctcggtgat cggcgcggcc cggccgttct cgcaacggag
      781 gcccggcagc atcagcagcg ccgacgcatc cgcgagcgcc gcggtggcgt tttcaaccgc
      841 cggccatccc gtgtacacca tgagccccca atcgaacgtc gtcgacccgc cgggcctggt
      901 tgctggctgc ggcggcagat ccttggcctc gacctcctgg atcgaaaaga ccagattcga
      961 gttgtggaag aacacgccgc gcatcaattg agcgagattg ccggttggcg aataccaact
     1021 cggtgagggc gcgctgcttg ctcccactcc gcgaccagtc ggccagccga tcgggctgtt
     1081 cgtcgcacca gccgccggaa agtcggtctg gccggcggga aagccattgc tcttgagaac
     1141 atacgcgacg agatcggcag cctgctctga tgtcagttgc gcgttcgcct cagccctggg
     1201 cggcatggcg tagcgaatct ggatgaaaag cgctgagagc ggctccatcc ggaacttgtt
     1261 cgtgaacagg tcgccggcca gcgctggcgc tgacgcgcca gccagcgacg ggccgtgaca
     1321 gccggcgcag cgttgcgcgt acagcgcctg gccgcggcgt gcttgcgcct cagagtagac
     1381 gccgtcctgc accgttagcg gtgcctgtgc agctgcctgc gtaccagcct gtgcaccgag
     1441 cgatgtcgcc gtgatgagca gtgtgacgaa cgtcagcgta gccagcgtcg taacgaatac
     1501 gcctcggaca tatgacatgc gcggtattgt aaccacaacg cacacgaaga tgatcgaaga
     1561 tcgcgaagag gaaaagcgcg aacgcgttcc gtgtctcctt attgcctgcc tgcgccgcgg
     1621 ccaggaccgg gggccgcggg agccgcaggg gccgcaaact ccgccgccac cgggaagttg
     1681 ccctcgtacg gaggccagtt cggcaccggc agatcgttgg tcggatagaa catggacgcc
     1741 cagatattgc gaatcttgtt gttatcgacg aagaaccatt cggccaccag attccgcatg
     1801 agcgttgtgc cgggcttgcg caggaagatg ccgaagccca ccacggcctg ctgctcgacg
     1861 tcgacgaccg gatagcgccg cgccgaaatc agcgagacgt tgaagttcgt caggttggac
     1921 gtgcaatcgc ccctaccgcc cggaccgccc ctggcggcgg cgcccgcgtt gggattcggt
     1981 cccgcggtgt gggtgccgtt ttcattgcgc gtgcagcctt catgggccat gatgatcgag
     2041 ccgtcgtgcg tcgtcagccc gtcgaagtaa ctgttggcga tcgcgatcag cacctcgcgc
     2101 gatgcccgct gattctgcgg cacggtgcgg tccggcggag gctcggcgat gaaccccgcg
     2161 aggttgtaga gattccctcc cgcagtgccg ctggggccga tggcgccctc gcgagcaatc
     2221 acccactcgg cttccgtaat ctgacgattc tctacccgca gccgcagcgt cgcgatgtcg
     2281 gtcgcctgac cttccttgat caacccgaga tacgcggcct gcccgctgac cggatcgaaa
     2341 tacttccgct gaatatcgcc cagcgccgtg atcgactgcc aggtgcccgt tcccgggcga
     2401 accaccacgc cattatcggt ctgccgaaag ccgacgaaca acggagccgc gctggtgtca
     2461 tgcttcacca cggcgttcag atactggtcg agcgttgtct tcagacacgc gcggtcgcac
     2521 gtctgtgcgg acgcgatgcc cgtcgcgaac gcagcgatca aaagaaaacg ggcagaatgc
     2581 attagataac gggcagaatg catggcgaaa cctccgcccc acattctgcc ccaatctgta
     2641 cagtgtttgc caggttactt cgcccactcc tcgtgctgga atgcccgcgc tgtagcggcc
     2701 gtatgaacat catcgcggcc atcgagtctc cgccgatcga accgatggtg gctctttccc
     2761 gcaagcgcca agatggaggg ccgagccaga aacgtgtatc ggtatcccga tggtcggctc
     2821 tgcgaccaag ccatatggag tctaacgccg accgacgcgc gaccagtggg ctaccgaaaa
     2881 gcgagctcag ttgacgtatt accgccatcc cttcatttgg ctattgggat gattccaaat
     2941 tccatcagtc cggtatgccg acttaccaac aactataggt aatatcttct ataggtaata
     3001 tcttccttca caagtccaca cctaaccacc tgccataagt tacagttctc ctctttcctg
     3061 gttccctgaa gtatgattcg tgcgcctaag tctcgacgtt gtgtcgctga tttgttgtgc
     3121 gtcggtatgg tgagcacgcc tggcgacatc cgcgagccgt cccaacgaac gcgagttcaa
     3181 agtgattgtc gaaaattcaa gcgagctggc tcaaggcgtg tgcaaccgtg agtgcgcacc
     3241 attagcgaca tcctgccgtc gccgctagcg cacaggtatc gcaagagtaa ggagaggaac
     3301 gtatgggatt gaattggagg aagttcattt tttcgggaag ggcgctgctg atcatgatga
     3361 ccctgttcgt gatgt
//
LOCUS       DBNHXB010000416         4796 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4319577, whole genome shotgun sequence.
ACCESSION   DBNHXB010000416 DBNHXB010000000
VERSION     DBNHXB010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4319577"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="AC162G_16535"
     CDS             complement(<1..184)
                     /locus_tag="AC162G_16535"
                     /inference="COORDINATES: protein motif:HMM:NF014047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HIR5077465.1"
                     /translation="MSQRTQPPAGLVLHLDAMTYGGDAIGRAGGKAIFVRGGIAGETV
                     RAQIVEDRGRFARAEAA"
     gene            complement(398..2893)
                     /locus_tag="AC162G_16540"
     CDS             complement(398..2893)
                     /locus_tag="AC162G_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR5077466.1"
                     /translation="MTNKLDRFTQRARRVLTYAQEEAERLSHNYIGTEHLLLGLMREE
                     SGVAGRVLRNLGVQPPRVMEMVERITGPGRRTPFSKIDLTPRTKRVIELAVEEARRMN
                     HHYIGTEHLMLGLVRQGDGVAMDILRQLGVTPEQIRRELTKELQDGAQSYTASRESRA
                     QQKKDRAKTPLVDQLATDLTAAAEEGKLDPVIGRQTEIERVIQILSRRTKNNPALIGE
                     PGVGKTAIVEGLAQRIVNGQTPESLLGKRVLQLDVGSLVAGTMYRGQFEERLKRVIEE
                     LKNSDAILFIDEAHMLVGAGAAGSAIDAANILKPALARGELQCIGATTLNEYRKYIES
                     DAALERRFQPVLVDEPTIDETIDILKGIRERYEQHHKLRITDAALEAAARLAMRYIPD
                     HFMPDKAIDLIDEASSRVRMYKAPQTKSLQEAHGELKTIQKEKERALEERRYDDAADL
                     RNREEDLIEQIDGLRTNWDSSDQVAMVTPEDIAEMVSMWTGIPVTRIAGTESERLLHM
                     EDDLHKRIVGQREAIEAISKAVRRARAGLKDPRRPIGSFMFLGPTGVGKTELTKALAE
                     LLFGSEDALIQIDMSEFMERHNVSRLVGAPPGYVGYDEAGQLTETVRRRPYSIVVFDE
                     IEKAHPEVFNMLLQILEEGHLTDARGRKVNFRNTIIVMTSNIGADLIQRQTSIGFNIK
                     RDEHQEANLAYAEMRKKLTESLKRTFRPEFINRLDSVIVFRALSKEEIKEIVGLELIK
                     VQERLKDHRIQLEATDAAKALLAEKGYDQDYGARPLRRVIQSEVEDRLSDGFLAGQFK
                     EGDTIVIDLLEGNLDFRSVRNPEAPVIEEPVLA"
     gene            complement(3060..4406)
                     /locus_tag="AC162G_16545"
     CDS             complement(3060..4406)
                     /locus_tag="AC162G_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR5077467.1"
                     /translation="MVDQEEHSASERSTPQNEDHTMQQLLDQQDFAFKEHKRGDALNG
                     TIVGIEANEILVDIGSKSEGVINIREMEGIPNEVLAELKVGDPVQAVVVAPEDRNGRV
                     VLTLSRAHTDRDWREAETLYGSQQVFEGVVAGHNKGGLIVKIGKVRGFVPASQLVSRG
                     SRGEGGEPGANSKENRLAALTGQKLQLKIIELDRQRNRLILSERAAMRDWRKTQKEKL
                     LSELQEGDVREGRVISLAEFGAFIDLGGADGLAHLSELSWKRVNHPREVLKVGQIVRV
                     QVIHIDPERKRIGLSLKRVEADPWSNLESRYQVGQLVEGTITKLAKFGAFARIQGDEE
                     IEGLIHISELSDSHIAHPKEVVREGQLVTLRIIRIEPHERRLGLSLKRVAKGEYVDSD
                     WQRGLAEATAEVEPATPGAPEPVPTAAPTPEPEPPESESTEAESTEGSLESDVTGA"
BASE COUNT          759 a         1557 c         1456 g         1024 t
ORIGIN      
        1 cggccgcctc ggcgcgcgcg aaccggccgc gatcctcgac gatctgagcg cgcacggttt
       61 cgccggcgat cccgccgcgc acaaaaatcg ctttgccgcc tgcccggccg atcgcgtctc
      121 cgccgtaggt catggcgtcg agatgaagca cgaggccggc tggcggctgg gtacgttggc
      181 tcataaacga acaagcagaa agaggccgtc gagcctcgcc ggtacacaaa aagaggctgc
      241 cgaccagcct atcagcagcc cctcttccat ttgcgagcgt gtctaggcgc aatactgttt
      301 agatcggaca gcggaggcgg ctcccatgct tgtcctgagc gacgccgaag ggacgccggg
      361 cgtgggagcc cggcccgcta gctaaacggc attgtgtcta ggcgagcacc ggctcttcga
      421 tcacaggcgc ttccggattc cgtactgagc ggaagtcgag gtttccttcc aggaggtcga
      481 tcacgatcgt gtcgccttcc ttgaactggc cggccaggaa tccatccgac agccggtcct
      541 ctacttcgct ctgaatcacg cgccgcagcg gccgggcgcc gtagtcctgg tcgtagcctt
      601 tctcggccag gagcgccttg gccgcgtcgg tcgcctcgag ctggatgcgg tgatccttca
      661 ggcgctcctg aaccttgatc agctccaggc cgacgatctc cttgatttcc tctttgctga
      721 gcgcgcggaa aacgatcacg ctgtcgaggc ggttgatgaa ctcgggccgg aaggtgcgct
      781 tcagcgattc ggtcagcttc ttgcgcatct cggcgtacgc caggttggct tcctgatgct
      841 cgtcacgctt gatgttgaac ccgatcgacg tctgccgctg gatgaggtcc gcgccgatgt
      901 tgctggtcat gacgatgatc gtgttgcgga aattcacttt gcggccgcgc gcgtcggtga
      961 ggtggccctc ctccaggatc tggaggagca tgttgaaaac ttcggggtga gccttctcga
     1021 tctcgtcgaa gacgacgatc gagtaggggc ggcgacggac cgtctccgtc aactggccgg
     1081 cttcgtcgta gccgacgtag ccgggcggcg cgcccaccag gcgcgacacg ttgtgccgct
     1141 ccatgaattc gctcatgtcg atctgaatca gcgcgtcttc gctgccaaac agcagctcgg
     1201 cgagcgcttt ggtcaattcg gttttgccga cgccggtcgg gccgaggaac atgaatgagc
     1261 cgatcggccg ccgagggtcc ttcagcccgg cgcgcgcccg gcgcacggct ttcgagatcg
     1321 cttcgatggc ctcccgctgg cccacgatac gcttgtgcag gtcgtcttcc atatgcagca
     1381 gccgctccga ctccgtgccg gcgatgcgcg tgaccgggat gcctgtccac atgctcacca
     1441 tctcggcgat gtcctcggga gtgaccatcg ccacctggtc gctgctgtcc cagttggtgc
     1501 gcagtccgtc gatctgctcg atgaggtctt cctcgcgatt gcgcagatcg gccgcgtcgt
     1561 cgtatcggcg ctcctccagc gcgcgctctt tttccttctg aatggtcttc agctcgccgt
     1621 gcgcctcctg caggcttttc gtctgcggcg ccttgtacat gcgcacccga gacgacgcct
     1681 cgtcaatcag gtcgatggcc ttgtccggca tgaagtgatc ggggatgtag cgcatcgcca
     1741 gccgggcggc tgcctctaac gccgcgtccg tgatgcgcag cttgtggtgc tgctcgtagc
     1801 gctcgcgaat gcccttgagg atatcgatcg tctcgtcgat ggtcggctcg tcgaccagga
     1861 cgggctgaaa acggcgctcc agggcggcgt cgctctcgat gtactttcgg tattcgttga
     1921 gcgtggtcgc gccgatgcac tgcaattcgc cgcgtgccag cgccggcttg aggatattgg
     1981 ccgcgtcgat ggcgctgccc gccgcgccgg cgcccaccag catgtgggcc tcgtcgatga
     2041 agaggatggc gtcggagttc ttaagctctt cgatgacgcg cttgaggcgc tcttcaaact
     2101 ggccgcgata catggtgccg gcgacgagcg agccgacgtc cagttgcagc acgcgcttgc
     2161 ccagcagcga ttcgggcgtc tggccgttga cgatgcgctg ggccaaccct tcgacgatgg
     2221 cggtcttgcc cacgcccggc tcgccgatga gcgccggatt gttcttggtg cggcgggaca
     2281 gaatctgaat cacgcgctcg atttccgtct gtcgcccgat caccggatcg agcttgcctt
     2341 cttcggcggc ggccgtcagg tcggtcgcca gttgatccac gagcggcgtc ttcgcgcggt
     2401 cctttttctg ctgagccctg gactcgcggc tggccgtgta gctctgcgcg ccatcctgaa
     2461 gctctttggt cagctcgcgg cgaatctgtt caggcgtcac gcccaactgc cggagaatgt
     2521 ccatcgccac accatcaccc tgtcgaacca gccccagcat cagatgctcg gtgccgatgt
     2581 agtggtgatt catgcggcgc gcttcttcca ctgccagctc aatcactcgc ttggtgcgcg
     2641 gcgtcagatc gatcttgctg aacggggtgc gacggccagg ccccgtaatg cgctctacca
     2701 tctccatgac gcgaggcggc tgcacgccca agttgcgcaa gactcgcccg gcaactccac
     2761 tctcttcgcg catcagcccc agcaggaggt gctcggtgcc gatgtagttg tggctgagac
     2821 gttcggcctc ttcctgagcg taggtcagga cacggcgggc gcgctgcgtg aagcgatcga
     2881 gcttgttggt cactgcaatc ctccatcccg atctccgtcc cttgcgggga tcgagattgt
     2941 tcagacatcg gttagattaa ttgggaacgt cggccggcgt tcgcgcgcga cgtcctgtag
     3001 aaatatatca cgcttttcga ttgcggcgtg atgggagata tgggggtaca ggtcggcctt
     3061 tatgcacctg ttacgtcgga ttccaggcta ccctcagtcg attccgcttc cgtcgattca
     3121 ctctcgggcg gctccggctc aggtgttgga gccgcagtcg gcacgggttc aggcgcgccc
     3181 ggcgtggccg gctcgacttc ggctgtggcc tcggccaggc cgcgctgcca atccgaatcg
     3241 acatattcgc ccttggccac gcgtttcagg ctcaacccca ggcgccgctc gtgcggctcg
     3301 atgcggatga tacggagcgt gaccagttgc ccttcccgga cgacttcctt gggatgcgcg
     3361 atatggctgt cggacagctc tgagatgtga atcagcccct cgatttcctc atcgccttga
     3421 atgcgcgcga aggcgccaaa cttggccagc ttcgtgatgg tgccttccac cagttgccca
     3481 acctggtaac ggctttccaa attagaccac ggatccgctt ccacccgctt gaggctcaag
     3541 ccgatgcgct ttcgctccgg atcgatgtgg atgacctgca cccgcacgat ctggcccacc
     3601 ttgaggactt cccgggggtg gttgacgcgt ttccaggaca attccgagag gtgcgccaat
     3661 ccatccgcgc cccccaggtc gatgaacgcg ccaaactcgg ccaggctgat cactcggcct
     3721 tcgcgcacgt cgccttcttg gagttcggag aggagcttct ccttctgggt tttgcgccag
     3781 tcgcgcatgg cggcccgctc ggacaagatc aggcgattgc gctggcggtc aagctcgatg
     3841 atcttgagct gcagcttctg tccggtcaag gccgcaagcc ggttttcctt cgagttggcc
     3901 ccaggctcgc cgccctcgcc ccggctgcct cgcgacacaa gctgggacgc cggcacgaat
     3961 ccccgcacct tgccgatttt gacgatcagt ccacccttgt tgtgtccggc gacgacgccc
     4021 tcaaacacct gctgcgagcc gtatagcgtt tccgcctctc tccagtctcg atcggtgtgc
     4081 gcgcggctga gagtcagcac gacgcgcccg ttgcgatcct ccggcgcgac gaccacggcc
     4141 tgcaccgggt cccccacctt cagctcggcc agcacctcat tcgggatgcc ttccatctcg
     4201 cgaatgttga tcacaccttc ggatttcgat ccgatatcga ccaggatctc gttggcttcg
     4261 atgccgacaa tggtgccgtt caacgcgtcg ccacgcttgt gttccttgaa ggcgaagtct
     4321 tgctggtcga gcagttgctg catcgtgtgg tcttcgttct gaggcgtgct cctttcgctc
     4381 gccgaatgct cttcctgatc caccatcacc gcccccgtcc ggacttaata atgacaaaat
     4441 tataaaccta ttccaatcaa atggcaaaca acctgtaatt ttagccagtc ccgacatgct
     4501 cagccgccag gcacaccgct tcggctcgcc gcgccgtccc ttcgacaggc tcctcactcc
     4561 cttcggtcgg ggacttcgca gggcaggctt cgcggttcaa atgccgtccg agtcgaagcg
     4621 gctgaccggg aactgattgc tgattactga ttactgactc tcaacgcctg cagaatcttc
     4681 tcgccggtga tgggcagtga ggtgagccgg gcaccggcgg cgtgatagac ggcattcgcc
     4741 agagtggcgg gcggttcgat gacaggcgac tcgcccaggc ccttgacacc gaacgg
//
LOCUS       DBNHXB010000417         3954 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2474374, whole genome shotgun sequence.
ACCESSION   DBNHXB010000417 DBNHXB010000000
VERSION     DBNHXB010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2474374"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..2576
                     /locus_tag="AC162G_16550"
     CDS             150..2576
                     /locus_tag="AC162G_16550"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5077468.1"
                     /translation="MRWLSRQLCAAVRALAVVFLVACAAPPEFDAAYTRVTPARLTPG
                     DAIPQGQQLLLSLGLASLLFIALVAIAAVGIHRFMRERQRAEKVRAATYRISEAALST
                     PSLDALFRAIHDIVGELMPAQNFYTALYDPATDRITFSYAVDQVETEPWTPKKSDKGL
                     IEYVLRTGKPLLASPDVFEALAQAGEVELVGAHPIDWLGVPLNIQDRTIGVLAVQTYT
                     GDVRYHEEDRDILMFVATQVAMAIERKRAEEALRASETKLRYLVEQLPSIIYVDDATR
                     EPGITLYVSPQIETLLGIPPAEWLAADMSLWSEHIHPDDRKRVVAEYLRCFTNREPFV
                     CVYRMITRQGEIKWFHDEAIPLPAEADQPCLVQGVMFDITDVKRAEEELHRYAQRLEE
                     MHAIDRAILAAQSPVDIARAAVLRLRHLTGCERAGVALFDFENQTARSIVVEADQPAG
                     PPEGAVFPLEALPPLDELRRETYQPDLTALAKRAPIFDDLLAAGMRSTISVPLIIEGQ
                     LTGHLFLTATRPAAFDAAAMEMAREVAVQLAVALQEAQLREELQRHAEELEQRVAERT
                     RELAEANTRLTELDRLKSKFVSDISHELRTPIMNLTMHLDLLERGKPDKHEHYIAVLK
                     AQVARLGRLVESILDLSRLEQGAAHPAFESLNFNALVEQVIGAQRLRAEALGLALIFE
                     PCAHTPAVRGVFDQLAQVVTNLVSNAISYTPSGEVRVSTCCDVERGPVCLQVEDSGIG
                     IDPEDLPYLFDRFYRGRRAAHSDIPGSGLGLAIVKEIVESHDGQIEVESQVGRGSRFL
                     VWLPKEDAKL"
     gene            2702..>3954
                     /locus_tag="AC162G_16555"
     CDS             2702..>3954
                     /locus_tag="AC162G_16555"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5077469.1"
                     /translation="MIQTSGRSQRESFDAEQKVGLLRALNAAAASLQRSAHSEFEVYR
                     AVKEQIVGLGLRGSLWALDEAGDRLTLRAVAYPGQALASLERLAGLKAEGFEFAAAQV
                     DVFRQVVESGQAVFASETVGLLAQILPRAVRPFAERIFSMIENAPGIFAPLVIEGRVR
                     GVLSAAGAGLTEEDVPAVEAFANHVAVALDNAYLFAAAQEELMERRRAEQVQNAAYHI
                     SEATLTASDLEALFRSIHHIISELMPAGNFYIALYDETTDLLSFPYFVDEYDEWSPPK
                     KPEKGLTEYVLRTGKPLLASPDVFDDLVRRGEVALVGVDSIDWLGVPLKIGEQTIGVL
                     AVQTYTEGVRYSDEDKAILTFVSAQIALAIGRKRAEDALRESVNKYRMLVEQAADVIS
                     IADPQGDYIEVNSKACELLGYTREEL"
BASE COUNT          721 a         1230 c         1291 g          712 t
ORIGIN      
        1 gggttaacag acaagcccgt cctgacagca ggcggaattt ggaatgtatt tgcaatgagt
       61 ctttgggcat cgggtggaat gtttcggata taatcatcct gcgactcttc gcagtcgggc
      121 aactcaccgc caacgggagg ggagctgtga tgcgctggct gtctcgccag ttgtgcgccg
      181 ccgttcgagc gctcgcagtg gtgttccttg tggcctgcgc cgcgccgccc gagttcgacg
      241 cggcgtacac gcgcgtcacg ccggcccgcc tgacgcccgg tgatgcgatc ccccagggcc
      301 agcagttgct gctgtcgctc ggactggcct cgctgctgtt catcgccttg gtcgccatcg
      361 ccgctgtcgg cattcatcgc tttatgcgcg agcgccaacg cgccgagaaa gtgcgggcgg
      421 ctacctaccg tatctcggag gcggcgcttt cgaccccttc gctcgacgcg ctgttccgcg
      481 ctattcacga catcgtcggt gaactgatgc cggcgcagaa cttctacacc gcgctgtacg
      541 accccgcgac cgatcggatc acgttctctt acgctgtcga tcaggtcgag accgagccgt
      601 ggacgcccaa gaagtccgac aagggcctga tcgagtacgt gctccgcacc ggaaagccgc
      661 tgctcgcctc gccggacgtg ttcgaggcat tggcgcaggc gggcgaggtc gagctcgtcg
      721 gcgcgcaccc gatcgactgg ctgggcgtgc cgctcaatat ccaagacagg acgatcggtg
      781 tactggctgt gcagacttac accggggatg tgcgctatca cgaggaagac cgggacatcc
      841 tgatgttcgt cgccacgcaa gtggcgatgg ctatcgagcg caagcgggcc gaggaagcgc
      901 tgcgcgcctc ggagacgaag ctccgctacc tggtcgagca gctccccagc atcatctacg
      961 tcgacgacgc gacgcgcgag ccgggcatca cgctgtacgt cagcccgcaa atcgagacgc
     1021 tgctgggcat cccgcccgcc gaatggctcg ccgccgatat gagcctgtgg tcagagcata
     1081 tccaccccga cgaccgcaag cgcgtcgtgg ccgagtatct gcgatgcttc accaaccgcg
     1141 agccgttcgt gtgcgtttac cgcatgatca cgcgccaggg cgagatcaaa tggtttcacg
     1201 atgaggccat cccactgccc gccgaagccg accagccgtg cctggtgcag ggcgtgatgt
     1261 tcgacatcac tgacgtcaag cgtgccgagg aagaactgca tcgatacgcc cagcgactgg
     1321 aagagatgca cgcgatcgac cgcgcgattc tcgcggcgca gtcgccggtc gacatcgccc
     1381 gcgcggccgt gttgcggctg cgccacctga ccggctgcga acgggccggc gtcgcactgt
     1441 tcgattttga aaaccagacc gcgcgatcga ttgtcgtgga agccgatcag ccagccggcc
     1501 cgccagaagg cgctgtcttt ccactcgagg cgctgccccc actcgacgag ctgcggcgcg
     1561 agacttacca gccggacttg acggcgctgg cgaagcgcgc gccgatcttc gatgacctgc
     1621 tggcggccgg catgcgcagc acaatcagcg tcccgctcat catcgaggga cagctcaccg
     1681 gccatctctt tctcaccgcg acgcggccgg ccgcgtttga tgcggcagcc atggagatgg
     1741 cgcgcgaggt cgccgtccaa ctggcggtcg ccttgcagga agcgcagctg cgtgaagaac
     1801 tgcagcgtca cgccgaggaa ctggagcagc gcgtcgccga gcggacgcgc gagctggccg
     1861 aagccaacac gcggctcaca gaactcgacc ggctcaagtc caagttcgtc tccgacatct
     1921 cgcacgagct gcgcacgccg atcatgaacc tgacgatgca cctcgacctg ctggagcgag
     1981 gtaagccaga caaacacgag cattacatcg ccgtgctcaa ggcacaggtg gcccggctgg
     2041 gacggctggt cgaaagtatt ctcgaccttt ctcgcctgga gcaaggcgcg gcgcatcccg
     2101 cgtttgagtc gctgaacttc aatgcgctcg tcgagcaggt catcggggcg caacgcctgc
     2161 gcgccgaggc actcggtctg gcgctgattt ttgagccgtg cgcccacacg cccgccgtgc
     2221 gcggcgtgtt cgatcagtta gcgcaggtgg tgaccaacct cgtgtcgaac gcgatcagtt
     2281 acacgccatc cggcgaggta cgcgtcagca cctgctgcga cgtggagcgc ggcccggtgt
     2341 gcctgcaggt ggaggatagc ggcatcggga tcgatccgga ggacctgccc tacctgtttg
     2401 atcggttcta tcgcggccgg cgtgctgcgc attcggacat ccccggcagc ggcctgggcc
     2461 tggcgatcgt taaggagatc gtcgagtcgc acgacggaca gatcgaagtg gaaagccagg
     2521 tcggacgcgg cagccggttc ctggtgtggc tgccgaagga agatgcgaag ttgtaatgca
     2581 gttgcatcgg ttctgcgtac aggcgtggac tataatctca cagatgcggg ctgtgcgaca
     2641 cgcatctcca tcggcgcgtg aacaatgtct ccgagggggt cgcgctcggg agagtaggaa
     2701 tatgattcag acgtctggcc ggtctcaaag agagtcgttt gacgcggagc agaaagtggg
     2761 gctgctgcgt gcgctcaacg cggcggcggc ctcgcttcag cgctcggcac attccgaatt
     2821 cgaggtctat cgagcggtca aggagcagat cgtcggcctg ggcctgcgcg gctcgctgtg
     2881 ggcgctcgac gaagcgggtg accgtctcac gctccgcgcg gtcgcctacc ccgggcaggc
     2941 gcttgccagc ctggagcgac tggccggtct gaaggccgaa gggttcgaat tcgccgcggc
     3001 acaggtggat gtctttcggc aggtggttga gagcggccag gcggttttcg cctcggaaac
     3061 agttggcctt ctcgcacaga tccttcccag ggccgtccgc ccgttcgccg agcgcatctt
     3121 cagcatgatc gagaacgcgc cgggtatctt tgcgccgctc gtcatcgaag gacgcgtacg
     3181 cggcgtgttg agcgccgcag gcgccggcct gactgaggaa gatgtgcccg ccgtggaagc
     3241 cttcgccaat cacgtcgcgg tcgcgctgga taacgcttat ttgttcgcag ccgctcaaga
     3301 agaactgatg gagcgcagac gggcggagca agttcagaat gctgcgtacc acatctcaga
     3361 agcgacgctc accgcctccg atctggaggc gctcttccgc tcgatccacc atatcatcag
     3421 cgagctgatg ccggccggca atttctacat tgccctgtat gatgaaacga cggacctgct
     3481 cagctttcct tacttcgtcg acgagtacga cgaatggtcg ccgccgaaaa aaccggagaa
     3541 ggggctgacc gagtatgtgt tgaggacggg caagccattg ctcgcttcgc cggatgtctt
     3601 tgatgatctg gtgcggcgcg gcgaggtggc gctcgtcggc gtggattcga tcgactggct
     3661 gggcgtgccg ctgaagatag gggagcagac gatcggcgtg ctcgcggtcc agacctacac
     3721 cgaaggcgtc cgttacagcg atgaagacaa ggccatcctt acgttcgtct ccgcgcagat
     3781 agccctggcg atcggacgca agcgggccga ggatgcgctt cgtgagagcg tcaacaaata
     3841 tcggatgctg gtggagcagg ccgcagacgt gatctccatc gccgacccgc agggagatta
     3901 catcgaagtg aactcgaaag cgtgcgagct gctgggctat acgcgcgaag aact
//
LOCUS       DBNHXB010000418         2059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_628502, whole genome shotgun sequence.
ACCESSION   DBNHXB010000418 DBNHXB010000000
VERSION     DBNHXB010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_628502"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="AC162G_16560"
     CDS             <1..195
                     /locus_tag="AC162G_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR5077470.1"
                     /translation="IVSVPDQARPVFIALRRLRDELAARFPQCDWRHLSMGMTDDFEV
                     AIEEGATMVRIGRAIFGERT"
     gene            214..477
                     /locus_tag="AC162G_16565"
     CDS             214..477
                     /locus_tag="AC162G_16565"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR5077471.1"
                     /translation="MAQLIGIVSLLFDVYSLILLARVLSSWFQVDPDNPLVQWLYRLT
                     EPVLAPIRRLLPPAGMIDFSPVVAFILIIVAKRIVLSVLVSLM"
     gene            477..782
                     /locus_tag="AC162G_16570"
     CDS             477..782
                     /locus_tag="AC162G_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HIR5077472.1"
                     /translation="MSGWKIDEMAGGVTFAVRVIPRSSRNQIAGVQGDALRVKLAAPP
                     VEGAANEALIGFLAERLGVRKSAVSIISGERNRSKTVRVDGVTRERVERVLLTGDLV"
     gene            complement(794..1756)
                     /locus_tag="AC162G_16575"
     CDS             complement(794..1756)
                     /locus_tag="AC162G_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006514625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HIR5077473.1"
                     /translation="MDPPPPKKLLDQVREALRLKHYSYRTEQTYVDWITRYILFHNKR
                     HPQEMGHPEVQAFLTHLAAEKNVAASTQTQALSALLFLYREVLHQDLGNIDALRARKP
                     KRLPTVLTRDETLRVISALKGTHQLMAKLLYGSGLRLMECARLRVKDIDFAQRQIVVR
                     DGKGEHDRVTVLPESLVAPLQEHLQRVKQLHDQDLDAGHGTVDLPYALERKYPNANRE
                     WGWQYVFPSERRSTDPRTGQVHRHHVDASGLQKAVRRAARLAKITKPVGPHTFRHSFA
                     THLLQANYDIRTVQELLGHKDVKTTMIYTHVLNRGGLAVRSPLD"
BASE COUNT          356 a          554 c          694 g          455 t
ORIGIN      
        1 atcgtcagcg tgccggatca agcgcgcccg gtgtttatcg cgctgcgccg cctgcgtgac
       61 gagctggcgg ctcgcttccc gcagtgtgac tggcgacacc tgtcgatggg catgaccgac
      121 gacttcgagg tcgcgatcga ggaaggcgcg acgatggtgc gcatcgggcg agccatcttc
      181 ggcgaaagaa cttagactgg gagcgatggc ggcatggctc aattgatcgg cattgtcagc
      241 cttctctttg atgtgtattc ccttatcttg ctggcccgcg tgctgtcgtc gtggttccag
      301 gtcgatccag ataacccgct ggtgcagtgg ctgtatcgac tgacggagcc ggtgctcgcg
      361 cctatccggc gtcttctgcc gccggccggc atgatcgatt tcagccccgt cgtggcgttc
      421 atcctcatta tcgtagccaa gcggatcgtg ttgagcgtgc tggtctcgct gatgtgatga
      481 gcggttggaa gattgacgag atggccggcg gcgtgacgtt cgccgtgcgc gtgattccgc
      541 gctctagccg caatcagatc gctggcgtgc agggagatgc cttgagggtg aagctggctg
      601 cgccgccggt cgaaggggcg gcgaatgagg cgctgatcgg ctttctggcc gaacggctcg
      661 gcgtgcgcaa gagcgcagtg tcgatcattt caggcgagag gaatcgcagc aagacggtgc
      721 gcgtggacgg agtgacacgc gaaagggtgg agagggtttt acttaccggt gatcttgtgt
      781 gatgacctgg ttcttaatcg agcgggctgc gcacggccag gccgccccgg ttgaggacgt
      841 gggtgtagat catggtcgtc ttcacgtctt tgtggccgag gagttcctgg acggtgcgaa
      901 tgtcgtaatt ggcctgaagc aagtgcgtgg cgaacgaatg acgaaaggtg tgcgggccga
      961 cgggcttagt gattttggct aaccgggccg ctcggcgcac ggccttttgc aggccgctgg
     1021 cgtccacgtg atggcgatgc acctggccgg tgcgggggtc ggtggaccgc cgctcggaag
     1081 ggaagacgta ttgccagccc cattcgcggt tagcattcgg atacttgcgc tcgagcgcat
     1141 aaggcaagtc caccgtgccg tgtcccgcgt ccaggtcttg atcgtgcaac tgcttgacgc
     1201 gttggagatg ctcctgcagc ggcgcgacga gactctcggg caagacggtc acgcgatcgt
     1261 gttcgccttt gccgtcgcgc accacgatct ggcgctgggc aaagtcaata tccttgacac
     1321 gcaggcgcgc acattccatg aggcgcaggc cgctgccgta cagcaatttc gccatcaact
     1381 ggtgcgtgcc tttgagggcg ctgatgacgc gcagggtttc gtcacgcgtc aggacggtgg
     1441 gcagccgctt gggctttctg gcgcgcagcg catcgatgtt ccccaggtct tgatggagca
     1501 cctcgcggta gaggaacagc agcgcgctga gggcctgcgt ctgcgtggag gcggccacgt
     1561 ttttttcagc ggccaggtgg gtcaggaagg cctgcacttc ggggtggccc atctcttgcg
     1621 gatggcgttt gttgtggaac aggatgtagc gcgtgatcca atccacgtag gtctgctcgg
     1681 tgcgatacga atagtgcttg agccgcaacg cctcacgaac ctggtcgagg agcttcttag
     1741 ggggcggggg gtccacgggt tacctccaca gatattgaca cgccagttgt ctgggattat
     1801 actgtgtgcg tcgatctcat gcggcggcgt tatgttccgc attttaccac cagaatccat
     1861 tcctgttcaa gctccattag gggacttgga cggaagttat tctggccaaa atctcccatt
     1921 ggggaatttt ccagaacagg gcttggcagc gtaggcggaa taaattccgt atactaacaa
     1981 gttaggccgc tcttcacgaa gcacgaggca gggcgggacc atgacctcac aatcattgtt
     2041 ccgtagcgcc ttttggatc
//
LOCUS       DBNHXB010000419         9501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2306727, whole genome shotgun sequence.
ACCESSION   DBNHXB010000419 DBNHXB010000000
VERSION     DBNHXB010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2306727"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="AC162G_16580"
     CDS             complement(<1..776)
                     /locus_tag="AC162G_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077474.1"
                     /translation="MADLQEIADLVAKHLPPSAERVRLRPLDERAREWAEAVAAATFA
                     QVSDEGECDAALGAHLARLPGAALRPGGRAIFLLFGEARPPDELATALAEAGFVRILI
                     EPVLDGAFILARGEPRSDVPDRNALVAALGAELSVAPAGGPLPRYLHLLVRQTPPTRG
                     WDPADSAPVTWEAMTVRDTASGESILIGFSSLAKAVAFMKPAALARVLPDVNKLPRLG
                     GETVAGWGMPVLLNPAFETLREELRFQFESPPLRVDPRME"
     gene            complement(1056..2069)
                     /locus_tag="AC162G_16585"
     CDS             complement(1056..2069)
                     /locus_tag="AC162G_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HIR5077475.1"
                     /translation="MTSFPVGKLPPRVLADLLAKFPPADRRVLLGPGVGLDCAVIDLE
                     NGNCLVAKSDPITFATDEIGWYAVHVNANDVATTGASPRWFLATLLLPEGRSDVALVE
                     SIFDQIQAACGEVGATLVGGHTEITFGIERPIVVGALLGEVARDRLVTPKGARPGDTL
                     LMTKRMAVEATSILAREKASELIGRFDLPYLHHARRFLHDPGISVVREAQTATAAGRV
                     HAMHDPTEGGLATGLHELAIAADVGLEIDARAVAIYPETQSLCAAFGIDVWGVIASGS
                     LLLAVHPDDSADILDALHAEGIEAQHIGRVAERERGVMVLDESGARPLRTFERDEITK
                     AFE"
     gene            2395..2691
                     /locus_tag="AC162G_16590"
     CDS             2395..2691
                     /locus_tag="AC162G_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007382670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR5077476.1"
                     /translation="MPTYTYRCSHCKYQFDKVQKFSDPPIERCPNCRRKSIHKVLHPA
                     PIQFKGSGWYVTDSKGSSSSTTASKPSKKKEPASETKSDADASSSRPTSSGDAD"
     gene            complement(2765..3064)
                     /locus_tag="AC162G_16595"
     CDS             complement(2765..3064)
                     /locus_tag="AC162G_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077477.1"
                     /translation="MIKLLMSWDIVQGRESEYFEFVLKEWAPGLQQLGIETHEVWFTV
                     YGDCPQILVSSLARDADTARQALASDDWKRLEKRLKEYVTGFSQKMIPAGVGFQL"
     gene            complement(3408..4373)
                     /locus_tag="AC162G_16600"
     CDS             complement(3408..4373)
                     /locus_tag="AC162G_16600"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077478.1"
                     /translation="MSRYTGRKALVVAVSIGMVLALLALPVSPSSAGALLQATAPARP
                     TLPSAPAPPTVTPAAPAESIAENPSIAGYVWVNGDIFVPAVGIPVRFRGDGFELAALT
                     DVNGYYQFENVGQDVGFLNVAGNGTGWKASVKDIALSPRPGESLRANFSASQGTPEKG
                     SALLSVSVNPNVFGAGQIVTVTVKATNNTDQQLSGVWLTHLLPDGLSVSGLSTSRGDS
                     QAVGQLTMAHLGDFAPGDSATFTIVAAAPGDGGPKGNFSAVVSMISHEGVAVQASASL
                     TGTGGPMTLPVTGMDNWLIAAGLLLAVMVVGVHQARRRSVRVSGP"
     gene            complement(4442..9268)
                     /locus_tag="AC162G_16605"
     CDS             complement(4442..9268)
                     /locus_tag="AC162G_16605"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077479.1"
                     /translation="MLPVAPLQPARARALVTRQDQSASPSISKSASAATVAFGDVFTY
                     TVVFTVPAGMTLSNMALVDDLPNNSPATEPNFTYVNGSSAGPAPGPGTPDITDFPPVV
                     TNGNHTLAWPLGTIANGSASPYVYSFTYRVTAGSGDNRVEVGTNAASLSYDGGSVNAS
                     ADVTVARAFFQQPFVSAYSANDTSATADNVEFLRAGDVVTFTVLITNVSPAGSVSTAY
                     TVVVSDTLPDWLVYGGPVGSTPPPDAITSVSGNRTQLGWQIGVVAPGATVGPLMFKAS
                     VTSTVAAGRRDNNDLRVEWRDLLTDPSADNSTDTIQMLSMNVAVSKQEADAFDPQGRV
                     VVNEPITYTVVVTIPAGTTVYSPAQVLDTLGDGLNYGGEVSHSGWTLPAVVGPSGNDT
                     SITWALTNTIAAATDVTMTFVFAGFSDGTRATGPINKGATLGNTAVMRWNVGSGSGAN
                     NPIPAQSGVGVQFVRPDIQPAKDSTPSAVISAGGEVIQYRITNLRNGSGVGDAAAASA
                     YDIVVTDTLPAGFSFVEAVPPPSGVFTVGSAVVITWGVAPSLPVGYFYNPPGITTTAT
                     TIYVTATAPATFAATSYTNTVVVQYSDQPGDTPGEQTYFFTPTKVLNVNFFETKSVEP
                     AGQLRIGDLITYTIADNVPRGASMYWPRHEDTMPQGVRFVAGSMALQGTTFVSSPIPI
                     TVPQGVQERLVWWTETISNLYGSDSLLVTTTFQARVTGINLSGGAVFSSANEFRGAPA
                     FSNQARVDWSTQDVSGTFDVFKNSNGVASQVVQPYLADSPFAKRFGGSTSGSTVVGAG
                     DLVTYHLVITNTGRGRAHDVVISDTLPFGLQLSDFSAVGRTFAGALFTPTFTAAPGPG
                     ATGSLGWVVDAIEPGDGNVIAPSTVFTLTYSARITDAIGAGATLPNEALLADYSSLPG
                     DEPFERHYSFLLGAVRTVTLTAPSPAIGKSVSVTGATWLEVMTYTLRFPQSPINATLY
                     NAAITDTVPSQITVLGVQAEGGVGAQVGFSGSVVTATAAAVAPLSQMIVTITGQIDPG
                     ASGTKVNVALLEWDDAATGGSRHVTASNPVTTTVLLPELNVTKSGPTLVGLGANVLYA
                     VVITNTGFVDAQNVRLIDDLPNELSFASFRASAAVTQTAGPDPLGFDLGTLAPRRSRT
                     IWITATSPLTLTPGILLTNTAIVSTTSPGENSADNTARHTAAVSGVVLDIRKTANVSS
                     IAPAGAIVYTIEYSNTGTAPATGVVVTETYDSNVTFVSAFPAPDSGSHVWNTPGTLNV
                     GERRAIVVTVTVAPALPNGTLITNLVTMSANSATARQASNVVDVVSAPALTIDKQGHG
                     ATMPGNTLVYRLNYANVGNAPATSVRLTETYPAELSFFAADPPPSFGDNVWDFGTLTP
                     PFSGTVVVTLSVGSGVPFGTVITNTVFLGSQETGEASDSFTTRVGPVFLPLVLRNFAT
                     APNLVVQKIVVAPASPSVAQPTVISVTIANVGTGAASAGFWIDLYVDPDDVPQPGQVW
                     NDIAPFGKAWVLRTPLLPGQSIVLDTTMPDDPADPAAVYSNWPGWFVSPGAHTLCAQV
                     DAFGGLGGLVVELDELDNLFGPAGVTVNPSGAPVHEGILGSFRPDPRPTPDPGIER"
BASE COUNT         1795 a         3088 c         2969 g         1649 t
ORIGIN      
        1 tcctccatgc gtggatcgac gcgaagcggc ggggactcga attgaaatcg cagctcctcg
       61 cgtaacgtct cgaacgccgg gttgagcagc accggcatcc cccaccctgc caccgtctcg
      121 cccccgaggc gcgggagctt gttcacatcc gggagcaccc tcgccagcgc cgccggtttc
      181 atgaacgcca cggctttggc gagcgacgag aatccgatca gtatggattc gccgctggct
      241 gtgtcacgca ctgtcatggc ctcccaggtg acaggcgccg agtcagccgg atcccacccg
      301 cgcgttggcg gcgtctggcg cacgagcaga tgcagatagc ggggaagcgg accgcctgca
      361 ggagccacgc tcaactccgc gcctagcgcg gcgacgagcg cgttgcgatc cggaacatcc
      421 gaacgtggct cgccgcgcgc caggatgaaa gcgccgtcga gcacaggttc gatcaggatg
      481 cgaacgaaac cggcttctgc gagcgccgtg gccagttcgt cgggcggccg cgcctcgccg
      541 aacagcagaa agatcgcgcg gccgccgggc cggagcgccg cgcccggcaa gcgcgccaga
      601 tgtgcgccca acgcggcgtc acactcgcct tcgtcgctga cttgcgcaaa ggtggcagcg
      661 gccacggcct ccgcccactc acgagcgcgc tcatccagcg ggcgcaggcg cacccgttcc
      721 gcgctcggcg gaagatgctt tgcaaccaga tcggcgatct cttgcaggtc agccatcact
      781 ctcaaactcc caacccttcg acccttcgac tacgctcagg gcgggcttcg ctcagagctt
      841 gccctgagcc tgcgaagggg caggcttcaa actccaaacg acaaacgtgt cattccgaac
      901 gcggtaagga atctctcgtc cgaccggcga acgcggcacg ccagagtaga gatgcctcgt
      961 cgttcctcct cggcatgaca cgtctggcgc ggatcatggg gtaacctccg tgaagattcg
     1021 tgtggattcg tggaagaaca acacggcctg agcagttact caaacgcctt cgtgatctcg
     1081 tcacgctcga acgtcctcaa tggccgcgcg cctgattcgt cgaggaccat cacgccgcgc
     1141 tcgcgctcgg caacgcgccc gatgtgttgc gcttcaatcc cttcggcgtg cagcgcgtcg
     1201 aggatgtccg cactgtcgtc agggtgcacc gccagcagca gggacccgga tgcgatgacg
     1261 ccccacacat cgatgccgaa cgcggcgcac agcgactgtg tctcaggata gatcgccacg
     1321 gcccgcgcgt cgatttcgag accaacatcc gcggcaatcg ccagctcgtg caggccggtg
     1381 gcgaggccgc cctcggtcgg atcgtgcatg gcgtggacgc gccccgcagc agtcgcagtc
     1441 tgcgcctcgc gcacgacgct gataccagga tcgtgcagga accgccgggc gtgatgcaaa
     1501 tacggcaagt cgaaccggcc aataagctcg gatgcctttt cgcgcgccag gatcgacgtc
     1561 gcttcgacgg ccatacgttt cgtcatcaga agcgtgtcgc cagggcgcgc acccttcggc
     1621 gtgacgagac gatcgcgcgc gacttcgccc agcagcgccc cgacgacgat cggccgctcg
     1681 attccaaacg tgatctcggt gtggccgccg acgagcgttg caccgacctc gccgcaggct
     1741 gcctgtatct gatcgaagat cgactccacc agcgccacat cggatcggcc ttcgggcagc
     1801 aggagcgtcg ccagaaacca gcgcggcgat gcgcccgtgg tggcgacgtc gttcgcgttg
     1861 acgtgcacgg cgtaccagcc gatctcgtcc gtggcgaacg tgatcgggtc ggacttggcg
     1921 accaggcagt tgccgttctc cagatcgatc accgcgcagt caagccccac gcccgggccg
     1981 agcagaaccc gccgatcggc aggcggaaat ttcgccagca ggtcagccag cacgcgggga
     2041 ggcagtttac cgaccgggaa gctcgtcatg ccgggattat atactaggca aaacaaaaga
     2101 ccgctctcac gagcggccct ttgttttgcg ccgtggcaga cccgaggtct ctagaaaacc
     2161 gaaggtgatg ccacggcgct gcgtttaccg ctattactca tcggcatcac ctcctccttt
     2221 ccataggatg gcttggaatg atatggcgat ggctatgcca tcgatgacag cactattatc
     2281 ccccaggcca gggctactgt caagtgggaa aatcagggta acgaccttgt cagcgattct
     2341 caaacgcggt aaaatccttg cgcgctgaat cacacgccgt gaaggtaatg gacgatgccc
     2401 acgtacactt accggtgcag ccactgcaaa taccagttcg acaaggtaca aaagtttagc
     2461 gatccgccga tcgagcgatg cccgaactgc cggcgcaaga gcatccacaa agtgttgcat
     2521 cccgcgccca tccagttcaa aggctccggc tggtatgtca ccgactcgaa gggatcgagc
     2581 tcgtccacga ccgccagcaa gccgtcgaag aagaaggagc cggcttccga gaccaagtct
     2641 gatgccgacg cttcgtcctc caggcccact tcgtccggcg acgcagatta gattcggatc
     2701 gcctcaaaac aaacgcggtc ggtagtcgtc gctgccgacc gcatcgcgtt tctcgtgcca
     2761 cgcgctacaa ttgaaatccg acgccagccg gaatcatctt ctgcgagaat ccggtgacgt
     2821 actctttcag ccgcttctca agacgtttcc agtcgtccga agccagcgcc tggcgcgccg
     2881 tgtccgcgtc gcgcgccagg ctgctgacca ggatttgcgg gcaatcgccg tagaccgtga
     2941 accatacctc gtgagtctcg atgccgagct gctgcaggcc aggcgcccac tccttcaaga
     3001 caaactcgaa atattcggac tcgcgcccct gcacgatatc ccaactcatc agcaatttga
     3061 tcattctctc ctcactcacc atcgagactc agtagaccca atttggctcg gcctggattg
     3121 ccaaatccag gccataaccg gggatttggc aatctcctca gagtatttgt ggtgaattat
     3181 atccgcttcc ggcgcatctg tttgtatcgg agcggtgtga gcaatccgcc gcccctaaat
     3241 tgccggattt tcacgcgcag caggcgggag cgggcgcgga aaacaggcca agcgcgcgtc
     3301 gtaaggccta tagaatgcac atcgctatcc aggctcgtag aacgggcatc cctatgcccg
     3361 ccgctgacgc ataggatgcg tcaaccacgg aggtcttaaa acagcctcta aggacccgat
     3421 acccggacag agcgccgccg cgcctgatgc acgccgacga ccataaccgc cagcagcagt
     3481 ccagcggcga tcagccaatt gtccattccc gttacgggca atgtcatagg cccacccgtg
     3541 cctgtcagcg aggcggatgc ctgcacggcg acgccttcgt gcgaaatcat cgacaccacg
     3601 gcggagaagt tccccttcgg gcctccgtcg cctggggcgg cggcgacgat cgtgaacgtg
     3661 gctgaatcgc ccggtgcgaa gtcgcccaga tgcgccatgg tcaattgccc gaccgcttgc
     3721 gaatcgccgc gactggtcga caggccggag acgctcaatc cgtcagggag caggtgtgtc
     3781 agccacacgc ccgacagttg ctgatcggtg ttgttggtgg ccttgaccgt caccgtgacg
     3841 atctgcccgg cgccgaacac attcgggttc accgagacgc tgagaagcgc cgatcccttc
     3901 tccggcgtcc cctgcgacgc cgaaaagttg gcccgcagcg actcgcccgg tctcgggctg
     3961 agggcgatgt ctttcaccga cgccttccag cccgtgccat tgcccgccac gttcagaaag
     4021 cctacatcct ggccgacgtt ctcgaactga taataaccgt tcacgtcggt cagcgcggct
     4081 aactcgaagc cgtctcctct gaaccgcaca gggatgccca ccgccgggac gaaaatgtcg
     4141 ccattcaccc atacgtaacc ggcgatcgac ggattctcgg ctatcgattc cgccggcgca
     4201 gcgggcgtga ccgtgggtgg agccggcgct gacgggagcg ttggccgggc aggcgcggtc
     4261 gcctgcagca atgcgcctgc gctcgacggc gagacgggca gagccagcag tgccaggacc
     4321 atcccaatcg ataccgcgac aaccagagcc tttcgaccag tataccgact catgagtcac
     4381 ctccaaaggc ccgatcctag cgaccggacc agcttaaaca gcgcacgggt gagagcgcag
     4441 gctacctctc tattcctggg tctggcgttg gcctgggatc aggccgaaaa gaccccaaaa
     4501 tgccctcgtg cactggcgcg ccggagggat tcaccgtcac ccccgccggg ccgaacaggt
     4561 tgtccaactc gtctaattct actaccaaac cgccaaggcc accaaatgcg tcaacctgcg
     4621 cacatagggt gtgcgctccg ggtgacacaa accagccggg ccagttactg tacacagccg
     4681 ccggatcggc tggatcgtca ggcatggtcg tatccaggac aatcgactgg ccgggcagca
     4741 gaggcgtgcg gagcacccag gctttgccga atggcgcaat gtcgttccac acctggccgg
     4801 gctgcggtac gtcatccggg tcgacataaa gatcaatcca gaagccagca gatgcagccc
     4861 ccgtgcccac gttggcaatc gttacactga taactgttgg ctgagcgaca ctcggtgacg
     4921 caggcgctac gacaattttc tgcacgacga gattgggcgc cgtcgcgaag ttcctgagca
     4981 cgaggggcaa gaataccggt ccgaccctgg tcgtgaagga atcggacgcc tccccggttt
     5041 cctggctgcc cagaaagacc gtattggtga tgaccgtgcc gaaaggcaca cccgagccga
     5101 cgctcagcgt cacgacgact gtgccgctaa agggtggtgt gagtgtaccg aaatcccata
     5161 cgttgtcgcc gaagctgggc ggcggatcgg ctgcgaagaa agacagctcc gccggatagg
     5221 tttctgtcag gcgaacgctc gtcgccgggg cgttgccgac gttggcatag ttcagtcgat
     5281 acacgagcgt gttgccgggc atcgtcgccc catgtccctg cttgtcgatc gtgagcgccg
     5341 gcgcgctgac cacgtcgacg acgttggacg cctgccgggc ggtcgcgctg tttgcgctca
     5401 tcgtgaccag attcgtgatc agcgtgccgt tgggcagcgc cggggccacg gtcacagtca
     5461 cgacgatggc gcgtcgctcg ccgacgttga gcgtacccgg cgtgttccac acgtggctgc
     5521 cgctatccgg cgcaggaaac gcggagacga aggtcacgtt cgagtcgtac gtctcggtca
     5581 cgacgacgcc cgtcgccggc gccgtgccgg tgttgctgta ctcgatcgtg tacacaatcg
     5641 ctcccgccgg cgcaatcgac gaaacattcg ccgttttgcg gatgtcgagc acgacgccgg
     5701 acaccgccgc cgtgtgccga gccgtattgt ccgccgaatt ctcgcccggc gacgtagtcg
     5761 aaacgatggc cgtgttagtg agcagtatgc ccggcgtgag cgtcagcggt gaagtcgccg
     5821 tgatccagat cgtgcgcgac cgccggggcg cgagcgtgcc caggtcgaag ccgagcggat
     5881 caggcccggc cgtctgcgtg accgccgccg aggccctgaa cgacgcaaag cttagctcat
     5941 ttggcagatc gtcgatcagc ctcacattct gcgcgtcgac gaagcccgtg ttggtgatga
     6001 cgacggcgta caacacgttc gcaccgagcc cgaccagcgt cgggccggac ttcgtcacgt
     6061 taagctccgg aagcagaaca gtcgtcgtga cgggattgga agccgtcaca tggcgggagc
     6121 ctcccgtggc cgcatcatcc cattcgagga gcgccacgtt caccttcgtc cccgacgcgc
     6181 cggggtcaat ctgcccggtg atggtgacga tcatctgtga gagcggcgca accgcagccg
     6241 ccgtcgccgt gacgaccgag ccgctgaacc cgacctgcgc gccgacgccg ccctccgcct
     6301 ggaccccgag caccgtgatc tgcgatggta cggtatcggt gatcgctgca ttatagagtg
     6361 tggcattgat aggcgactga gggaatctca gcgtatacgt catcacctcg agccaggtcg
     6421 cgccggtgac gctcacgctc ttgccgatcg ccggcgatgg cgccgtgagc gtgacagtcc
     6481 gcaccgcgcc caacaggaag ctgtagtgcc gctcgaacgg ctcgtcgccg ggcaggctcg
     6541 agtaatcggc cagaagcgct tcattcggca gcgtcgcacc cgcgccgatc gcgtcggtga
     6601 tccgcgccga ataggttagc gtgaaaaccg tggacggcgc aatcacgttg ccgtcacctg
     6661 gctcgatggc atcgaccacc cagccaagcg agccggtcgc gccagggcca ggcgcggcgg
     6721 tgaaggtggg ggtgaacaac gcgcccgcga acgttcttcc caccgcgctg aagtcgctga
     6781 gctgcagccc gaacggcagc gtatcgctga tgacgacatc gtgcgcccga cctcgaccgg
     6841 tgttcgtgat gacgaggtga taggtaacca agtcgcccgc gcccaccacg gtcgagccgc
     6901 tcgtcgatcc gccaaaacgt tttgcaaacg gcgaatcggc caggtacggc tgaaccacct
     6961 gactggcaac gccgtttgag tttttgaaca catcgaacgt gccgctgacg tcctgcgtgc
     7021 tccaatcgac gcgcgcctgg ttgctaaaag caggcgcgcc acggaattcg ttggcgctgg
     7081 agaagaccgc gccgcccgac aggttgatgc cggtgacgcg cgcctgaaac gtcgtcgtga
     7141 cgaggagtga gtcgctgccg tacaggttgc tgatcgtctc cgtccaccac accagccgct
     7201 cctgcacgcc ttgcggaact gtgatcggga taggactgct gacgaaagtc gtcccctgca
     7261 gcgccatcga gccggcgacg aagcgcacgc cttgcggcat ggtatcctcg tggcgtggcc
     7321 agtacatgct tgcgccgcgg gggacgttgt cggcaatcgt gtacgtgatc aaatcgccga
     7381 tgcgcaactg tccggcgggt tcgaccgatt tggtctcgaa gaagttgacg ttgaggactt
     7441 tggtgggcgt gaagaaatac gtctgctcgc cgggcgtatc gccgggctga tcggaatact
     7501 gcacgacgac ggtattggtg tagctcgtgg ctgcaaaagt ggccggcgct gtagccgtga
     7561 cgtaaatcgt ggtcgcggtc gtggtgattc cgggcgggtt gtagaaatag ccgaccggca
     7621 gggaaggcgc gacaccccac gtgatcacaa ccgcgctgcc gaccgtaaac actccgctcg
     7681 gcggcggcac agcctcgacg aagctgaaac cggccggtaa cgtgtccgtg accacgatgt
     7741 cgtacgcgct cgcggctgcg gcgtcgccga cgcctgaccc gttgcgcaag ttcgtgatcc
     7801 gatactgaat gacctcgccg cccgccgaaa tcaccgccga cggcgtcgaa tccttggcgg
     7861 gctgaatgtc gggccgcacg aactgaacac caacgccgga ctgcgccgga atcgggttgt
     7921 tcgctcctga gccgctgccg acgttccagc gcataacggc cgtgttgccc agcgttgcgc
     7981 ccttgttgat cggaccggta gcacgcgtgc cgtcagagaa ccctgcgaac acaaacgtca
     8041 tcgtcacatc ggttgccgcg gcgatcgtgt tcgtcagcgc ccaggtgatg gacgtgtcgt
     8101 tgccgctcgg cccgacgacg gccggcagcg tccagcccga gtgcgatacc tccccgccgt
     8161 agttcagccc atcgcccagc gtgtcgagga cctgcgccgg gctgtacacg gttgtgccgg
     8221 ccgggatcgt gacgacgacg gtgtacgtga tcggctcgtt gaccaccacg cgcccctgcg
     8281 gatcgaacgc gtcggcctcc tgcttggaca cggccacgtt cattgagagc atctggatgg
     8341 tgtccgtcga gttatctgcc gagggatcgg ttagcaggtc gcgccactcg acgcggagat
     8401 cgttgttgtc ccgccggccc gcggcgacgg tcgacgtgac gctcgcctta aacataagcg
     8461 gcccgacggt tgctccgggc gcgacgacgc cgatctgcca gccgagctgc gtccggttgc
     8521 cgcttacaga ggtgatggca tcgggtggtg gtgtcgatcc gaccggcccg ccgtacacca
     8581 gccagtccgg cagggtgtcg ctgacgacca ccgtgtaggc cgtcgacacg ctgcccgccg
     8641 ggctgacatt cgtaatcagg acggtgaacg tgaccacgtc gcccgcgcgc aggaattcga
     8701 cgttgtcggc cgtagccgac gtgtcgttag ccgaataggc cgagacgaac ggctgctgaa
     8761 agaatgcccg cgcaacggtc acgtcggcgg acgcattgac gctgccgccg tcatacgaga
     8821 gcgatgccgc gttcgtcccg acttcgacgc ggttgtcgcc gctgccggcc gtgacacggt
     8881 aggtgaacga atagacgtag ggagacgcgg agccattggc aatcgtgccg agcggccacg
     8941 ccagcgtatg attcccgttc gttacgaccg gcgggaagtc ggtaatatcc ggcgtgccgg
     9001 gaccgggcgc cgggccggcc gacgacccgt tcacgtatgt gaagttcggc tccgtggcag
     9061 ggctgttgtt cggcaggtcg tccaccagcg ccatgttaga aagcgtcatg ccggcgggga
     9121 cggtgaaaac gaccgtgtag gtgaaaacat cgccgaaggc gacggtagcc gccgacgccg
     9181 attttgatat tgaaggagag gcgctctggt cctgccgcgt cacaagcgcc cgggcgcgtg
     9241 ccggctggag cggggcaacg ggcagcatca acatgcgccc gtcaggggcg tgtagcccat
     9301 ccggcagatg cgctagccct gcggtcgctg ccaggccaga ttcagtggtg tcttcgccag
     9361 catttcgcgc ccactcccgc cggactgcca aatccggtgg ctggggcgcg tgcggattcg
     9421 ttttgttgga tggcaagcgc gtcgaaggct gattcgaggc taacgagtcg gcgcgcgtcg
     9481 tctgcacgct gatcgcgcaa a
//
LOCUS       DBNHXB010000420        14455 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3816657, whole genome shotgun sequence.
ACCESSION   DBNHXB010000420 DBNHXB010000000
VERSION     DBNHXB010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 14455)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14455)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14455
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3816657"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..654
                     /locus_tag="AC162G_16610"
     CDS             79..654
                     /locus_tag="AC162G_16610"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5077480.1"
                     /translation="MPDISTLIQRWQAGDERAAEALYNQFREPTFRLAYGLLGDPSDA
                     EDAAQDALAYALAHIGHYEPQRASFSTWLHTITVSRCRDRQRRRHPPSLSLDWLKGER
                     EHPDPAPGPEPRTVRAETHGEVWKAIQVLSPPLREAILLRYWAGHTFREMADILSCPL
                     PTAQSRVRLAYVQLRTRLTPADLNALEEGAR"
     gene            651..2663
                     /locus_tag="AC162G_16615"
     CDS             651..2663
                     /locus_tag="AC162G_16615"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR5077481.1"
                     /translation="MITHVSDEQLIGYTHQTLADDQREAMDRHLAGCPDCRARLTEHE
                     ALQRRIHHTLLAELKAAHPSVRMRYAAIAPRLQRSSQWARVWKRISGVVALTTFVVLM
                     AVLVTLFRSMSQPIAEPTPENVPIFRDITSISITALSGASQTLPATGRLEPNTGWFVA
                     RNDPQSYDIGIDYIVRRSDKASGYIKSRVADPQSFGNLMQTFRADAYRGKRLRLSVYV
                     KAEQVEGWAVVWMRVDGPPNEMLSLDNMQDRPIQGTRDWAKYELVLDVPEDSASIAFG
                     LLLLGKGQVWIDDVQFDGVGHETPTTATVRVEEQPLNLDFEADRAGWFTGGNSPQDFT
                     SGINRAVAHSGQASGYVKSKSLQPASAGMLSQMIKADAYRGRRIRLSGYIKTDQVGGR
                     AGLTLAIYGPLNQWIGSYEMRNGPVQGTTDWQPGEIVVDVPENSVKIMLGLSLDGTGQ
                     VWADDFRIELVGADVPTTGSSQAITPVPTPNWAAWPARPDDLDFEIVGGGKQPPAHWF
                     IAGSAPQDYEISVDAAIAHGGRASGAIQSRASAVASGFGTLAQVFQADAYRGQRLRLS
                     GYLKAEQVGGSAGLWMRIDDTHNAMLSFDNMQDRPIRGTRDWAQYEIVLDVPENSKGI
                     TFGVLLYGSGKVWIDDVLIEAVGADVPTTDLFAVRQPQPLNLGFED"
     gene            complement(2771..4120)
                     /locus_tag="AC162G_16620"
     CDS             complement(2771..4120)
                     /locus_tag="AC162G_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HIR5077482.1"
                     /translation="MATLMHPSPDMAAPSGLTAREWVARDRAVMSPSLTRAYPFVMAR
                     GRGSEVWDVDGKRYIDMNATIAVAATGHSHPAVVEAIQRQADQFLQYAMTDFYTPQTV
                     ELAEKLNRLRPMEEEVQVFFTNSGTETVEAAIKLARYHTARPNFIGFLGAFHGRSMGS
                     LAFTASKATQKKGFSPAMPGVTHVPYCYPYRPILASPEGADYGDTVVDYIEHVIFRSL
                     VAPTDVAAILLEPIQGEGGYVVPTPNFLPRLRALCDMYGILLIADEVQSGMGRTGKWW
                     AIEHWNVEPDILLSAKGIASGLPLGAMLARKSVMTWQPGSHGNTFGGNPVSCAAALAT
                     IDVIEKEGLLKHAAEIGEYLMDALAEIQVRHPTIGDVRGKGLMIGVEFVRDKEAKDPA
                     HDLSEDITQRAFQKGLLLLPAGVSTIRLSPALNIPKDLVDEALSIFEEAITEAEAAL"
     gene            complement(4338..6368)
                     /locus_tag="AC162G_16625"
     CDS             complement(4338..6368)
                     /locus_tag="AC162G_16625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR5077483.1"
                     /translation="MTAENATFVRRISLAALFILLLAFALRVYRLDAQSLWYDEGLSA
                     YLAALPPGETIAQSAITDHPPLHALLLNVWMRAAGSSEFSIRFASVFFGTLAVALAHA
                     LGRRVGGHRVGLIGAGLMAVAPMAVWYGQEARGYSLLITLILIAALAFLRWVIGDWRR
                     RVWLAYILSCTAALYTHYFAAFPIVAINLAFLVHLARSKLPDRYSVLRVPCSGDSSTP
                     HKPHAARDTQHATRSTQHKIRAWLLAQLAIAVLFLPWLPNALAQAASNATYFPGRVTW
                     DTVVFDTWRAFTAGELPSPALPDLSHLVSVLWLAIILAGLASRFIRRATTPHSNSHSD
                     PVLVFLLVVPLVLMSILAWERPKFAPRYLLPSLPAFVTLAALGIDALLRFRNRLLFVL
                     AAILLLSILALDLLVAAQIQLDPSVARPDVRSVAAYIEDHELSGDAILLVGGHQAPVF
                     DYYYRGSADVIPLPPDLLPAAQSPLDTRAVTRLADLAMKHPRAWLVLWQQNIADPTEV
                     ILSELQEQADHLEVGRNFHEMSLLLFDLRDAEFQAEPQQPVAVEFEEPIRLVGFDLNS
                     NRSLPGEALDLALYFESSGLIARNYHAFTHLVSPDGSIAAQADGIAGADSYPTSLWPA
                     GARMRNRFSIRLPPDLPPGDYRLRVGLYDDAGRLPLSTGGDAIDLATITIRP"
     gene            6686..7960
                     /locus_tag="AC162G_16630"
     CDS             6686..7960
                     /locus_tag="AC162G_16630"
                     /inference="COORDINATES: protein motif:HMM:NF024827.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR5077484.1"
                     /translation="MRHNKFIWFGVVAVVALVLAGCAGPQGEQGPPGPAGPPGPEGPQ
                     GPPGPAGAAGEAAAAAEVGAEYVGSQTCGQCHETIYETFMKSGHPYKLNKVVDGKPPV
                     YPFTELPSPPEGYTWDDISYVIGGYGWKARFMDQQGYIITDKPGATISDTNYLNQYNY
                     ANAVVGADAGWVKYKSGTPQLKYDCGTCHTTGYSAFPPDSHQDDLPGIIGTWAAPGIQ
                     CERCHGPGGNHVSDPGGVSMEIERSSALCGDCHIRGAVESVNAAGGFIEHHEQYEELF
                     QSKHITLDCVICHDPHTLVIQLRKAQESDPSVQTTRTQCQNCHFKQAQYQDSEVHPAI
                     GVQCTDCHMPRIAKTAWGDAEKFTGDIRTHLMAIDPKQIGQFSEDGTTALSQIGLDFA
                     CRQCHVEGGKATPKTDEQLIDKATGYHTPPQE"
     gene            8073..9197
                     /locus_tag="AC162G_16635"
     CDS             8073..9197
                     /locus_tag="AC162G_16635"
                     /inference="COORDINATES: protein motif:HMM:NF025890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5077485.1"
                     /translation="MSKRRLTIGVGLAGLALAALSLSAGGASATPAAPAPLAQGVDNE
                     TCLACHTQPDMRTTLPSGEELFLTIDRDVYNSSVHGEDGYACVQCHIGYTGFPHEPIQ
                     VQTRREFSVRQAASCSRCHQQQHEQTLVNAHGQAQAAGKVEAAVCTDCHGAHNVESFA
                     ERPRSAIPQTCQKCHSEIYALYEDSVHGAALIGEGNPDVPACTDCHGTHTVEGPSSVE
                     RFRLHSPEICAKCHADKELMAKYGISTEVFETYLSDFHGKTVLFQASVPGQETNKPVC
                     IDCHSVHNILPPADPASTVNIQRNLLTTCQKCHPSAQENFPAAWLGHYAPSRENAPLV
                     YFVDLFYKIFIPSVLGVMAVFVVTDAARRILNRRKRQGHE"
     gene            9190..10353
                     /locus_tag="AC162G_16640"
     CDS             9190..10353
                     /locus_tag="AC162G_16640"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR5077486.1"
                     /translation="MSELRKYLRFRRPHRVEHWVFMASFTTLAVTGLAQKYAEAPISQ
                     WIVVTLGGIESVRVIHRLAAITIMVVAVYHIGAVGYRLYVNRSRLHMLPGLFDVRNAW
                     QSLKYNLGLSKARVQQGRYGFEEKLEYWAVVWGTLIMVLTGFMLWNPIATTSILPGEV
                     VPAAKIAHGLEAVLAVVAIFLWHLYHVLIRTFNRSMFTGYVNEEQMLDEHPLELADIK
                     AGIAERPVDPQKKERRRVFFGVYGVVGTVLLVGIFVFVTFEKTAIETVPAPPPEQVTV
                     FAPLTPTPLPTRLPTATPAPVTAVTWAGGRGDLFKAKCTACHGSPSGLGGLDLSTYEG
                     ALKGGNSGLGIVPGDPEASGIVRIQSQGGHPGQLTDSELETVMQWIEAGAPEQ"
     gene            10409..12106
                     /locus_tag="AC162G_16645"
     CDS             10409..12106
                     /locus_tag="AC162G_16645"
                     /inference="COORDINATES: protein motif:HMM:NF015237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HIR5077487.1"
                     /translation="MKRIKRALRRFFLPSPGSPLWRRVLPWATLAGVFVLVSFSGAYA
                     WEYTNSPQFCGTTCHTMPPEYTAYLTSPHARILCVECHIGRDYLGSRVLRKAGDLKHI
                     AATIFTDYEFPIRAEDMRPARETCEQCHSPDKFADDSLREIRHFGDDKNNTPESIFLT
                     LKTGGGSKRQGLGRGIHWHVENKVFYLPADPHEQDIPYVRVVGDDGQVAEFVDVESGL
                     DPNTIDESKLEEMDCITCHNRITHLILPPEERVDQLLARQVIPSTIPDIRRQAVAALG
                     APYESMALAMNGIEGLRGYYQSVYPEFYTANQDQVEAVIATLQETYRNSVFPEQKADW
                     TTHANNIGHKSSPGCFRCHDGKHLTSEGESIRLECNLCHSIPVVAGADDFVVDIEISR
                     GPEPESHLNSNWIALHRDAFDTTCANCHTTEDPGGVSDTSFCSNSGCHGQTWEYAGFD
                     APALRAKLQGQLPPTPTPMPTPAGGLPLTYEAAIGPLFQGRCAACHGEDGIQGLDLTT
                     YEGVMAGSQNGPVVVPGDAEASLLVEKQSGAQPHFGQLTPDELQLVIEWIEAGAPEK"
     gene            12170..13630
                     /locus_tag="AC162G_16650"
     CDS             12170..13630
                     /locus_tag="AC162G_16650"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5077488.1"
                     /translation="MTASAVSLHRGLRAKIALGVALPIFVVLLSLSLFHIWRERHLAE
                     TQIESAALQMGEMTLGGLRHAMATGDDAALKQILSDVSRLDNIHQVQIIGLDGRVKAN
                     SLGRRVGEVQNLDQSGCVECHPASGEATARTARLPYPSGIMRVSLPIRNEADCAGCHT
                     PLNALLGTLLVDVSVTDLEKHLLDDLRVDLLLTSGITVLATLGIYVLIHRLVVRRIGA
                     MQKPLARFAQGSFSARMPVSLDPPDEIDALADAFNHMADELQRHTREQEERSQVRQRA
                     IVEERERIARELHDGMAQVLGYVSTKVMAARLMLKKQRAHEAEQHLLQLEEAAQRLFV
                     DVREAILGLKMAARSGAGLPSALREYTAQYHQLTGIPVDLSIDPAAEDLALPAETELQ
                     MVRIVQEALTNVRKHASAKRASVGLHRENGTLELLVRDDGVGFDPDGARLRDSGQPHF
                     GLGTMRERADAIGADFNLHSEPGAGTQVSVRLKIQG"
     gene            13635..14300
                     /locus_tag="AC162G_16655"
     CDS             13635..14300
                     /locus_tag="AC162G_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5077489.1"
                     /translation="MASVRVLVADDHSLFRDGIVSLLEAAGFQVIGQVGDGRAAVEAA
                     RRLHPDLVLMDLSMPEMTGLEALRAIRAEMPDVQVVMLTVSDEDSDLFEAIRAGARGY
                     LLKSLNADEFIELLEGLQRGDAAISRKTATRLIDGYAALSRQQTKPSESLLTPREMEL
                     LQLIVDGMSNKAIAERLSLSENTIKYHIKNILQKLGVQNRTEAATQALRSGLLSPTRH
                     VDP"
BASE COUNT         2828 a         4497 c         4674 g         2456 t
ORIGIN      
        1 ttaaagtgcc tcagattgac gtataacagt gtagaatgtc gatcgacatc tttagccttg
       61 ccgtgcctgg gcgatgatat gcccgatatc agcactctga ttcagcgttg gcaggccggc
      121 gacgagcgcg ctgctgaggc gctctataac cagtttcgcg agccgacctt tcggctggct
      181 tacggcttgc tgggcgatcc gtccgatgcc gaggatgcgg cgcaagatgc gctggcctac
      241 gcgctggcac acatcgggca ctacgagccg caacgcgcca gtttcagcac ctggctgcac
      301 acgattaccg tcagccgctg tcgcgatcgg caacgccgcc gtcatccgcc cagcctgtca
      361 ctggattggc tgaaagggga acgcgagcat cccgacccgg ccccaggccc cgagcctcgc
      421 accgttcgag ccgaaacgca cggcgaagtg tggaaggcga tccaggtgct cagcccaccc
      481 ctgcgcgaag cgatcctact gcgctactgg gccggacaca ccttccgcga gatggccgac
      541 atcctgagct gtccgttgcc gacggcccag tcgcgcgtgc ggctggccta tgtacagctg
      601 cggactcgcc tgacgccggc cgacctgaat gctctggaag aaggtgcacg atgatcactc
      661 atgtgagtga tgagcaactg atcggttata cccaccagac gcttgccgat gatcaacgtg
      721 aagcgatgga tcgccacctc gctggctgcc ccgactgccg cgcccgcctg actgaacatg
      781 aggcgttaca gcgacgcatc catcacacct tgttggccga gctgaaagcg gcccatccat
      841 cagtgcgcat gagatacgcg gccatcgccc ctcgcctgca acgctcaagt caatgggcga
      901 gggtgtggaa acggatctcc ggcgtcgttg cgctcacaac attcgtcgtg ctcatggctg
      961 tgctggttac tctctttcgc agcatgagcc agccgatcgc agagcctaca cccgaaaacg
     1021 tgccgatttt ccgggacatc acttcgatca gcatcacggc actctccggc gcgagccaga
     1081 cgctccccgc caccggccga ctcgaaccga acacgggctg gttcgtcgct cgaaacgatc
     1141 cccagagcta cgacatcggc atcgattaca tcgtccgccg cagcgacaaa gccagcggtt
     1201 acatcaaatc cagggtggcc gacccgcaga gcttcgggaa tctcatgcag accttccggg
     1261 cagacgctta tcgcggcaag cgcttgcgcc tgagcgtgta cgtcaaggcg gagcaggtgg
     1321 aaggatgggc ggtcgtatgg atgcgcgtcg atggcccgcc gaacgagatg ctgagcctgg
     1381 ataacatgca agaccggccg atacagggca cgcgcgactg ggcgaaatac gagctcgttc
     1441 tggatgtgcc ggaagacagc gccagcatcg ccttcggcct gctgctgctg ggcaaaggtc
     1501 aagtgtggat cgacgacgtg caattcgacg gcgtgggcca cgagacgccg acgacggcga
     1561 cggtgagggt cgaggaacag ccgcttaacc tcgatttcga agccgatcgc gccggttggt
     1621 tcaccggagg caacagtccg caagatttca cgagcgggat caatcgggcc gtcgcgcata
     1681 gcggtcaggc gagcggctac gtcaagtcga agtctcttca accggccagc gccggaatgc
     1741 tgtcgcaaat gatcaaggcc gatgcctatc gcggcaggcg gattcgcctg tccggctaca
     1801 tcaaaacgga tcaggtcggt ggccgcgcgg gtctgacact ggcgatatac ggcccgctga
     1861 atcaatggat cggctcctac gaaatgcgca acgggccggt gcagggtacg accgactggc
     1921 agccaggcga gatcgtcgtc gacgtgccgg agaacagcgt caagatcatg ctcggcttat
     1981 cgctggacgg gacgggacag gtgtgggccg acgactttcg gatcgaactc gtcggcgcgg
     2041 acgtgccgac gaccggttca tcgcaagcca ttacaccggt gcccacgccc aactgggcgg
     2101 catggccggc gcgacccgat gatctcgatt ttgagatcgt cggcggcggc aagcagccac
     2161 cggctcactg gttcatcgcg ggtagcgccc cacaggatta cgagatcagc gttgacgccg
     2221 cgatcgcgca cggcggccgg gccagtggcg cgatccagtc gagggcgtcg gcagtcgcgt
     2281 cgggctttgg gacgttggca caggtgttcc aggccgatgc ctatcgaggc caacggctgc
     2341 ggctgtccgg atatctcaag gccgagcagg tcggtggctc ggcgggtttg tggatgcgca
     2401 tcgacgatac gcacaacgcg atgttgagtt tcgacaacat gcaagaccgg ccgatacgcg
     2461 gcacacgcga ctgggcgcaa tacgagatcg tcttggacgt gccagagaac agcaagggga
     2521 tcacgtttgg cgttctcttg tacggctcgg ggaaagtgtg gatcgacgat gtgctgatcg
     2581 aggccgtcgg cgcagacgtg cccacgaccg atctattcgc ggtccggcag ccgcaaccgc
     2641 tcaacctggg ttttgaggat tgaaccagaa accgggtttc tgccagagac ccggtttctc
     2701 tagcaacgtt cttcgtgtgt ttgtgacttc gtggttaaca ggtcaacagg tcgtcgtccg
     2761 gagacgactc ctacagcgcc gcttctgcct ccgtgattgc ctcttcaaag atactcagcg
     2821 cctcgtcgac caggtccttc ggaatgttga gcgcaggcga caggcgaatg gtgctgacgc
     2881 ctgccggcag cagcagcaag cccttctgga acgcgcgctg cgtgatgtct tcgctcaagt
     2941 catgcgccgg atccttcgct tctttgtccc gcacgaactc gacgccgatc atcagaccct
     3001 tgccgcgcac atcgccgatc gtgggatgcc gcacttgtat ctcggccagc gcgtccatga
     3061 gatattcgcc tatctcggcc gcgtgcttca gcaagccttc tttctcgatg acgtcgatcg
     3121 tcgccagcgc cgccgcgcac gagaccgggt tgccgccgaa agtgttgccg tgcgatccgg
     3181 gttgccacgt catcacgctc ttacgcgcca gcatcgcgcc gagcggcagg cccgaagcaa
     3241 tgcctttggc cgagagcagg atgtccggct cgacgttcca gtgctcgatc gcccaccact
     3301 tgccggtgcg gcccatccct gactgcactt cgtcggcgat gagcaagatg ccgtacatgt
     3361 cgcacagcgc gcgcaggcga ggaaggaagt tgggtgtggg cacgacgtac ccaccctcgc
     3421 cctggatcgg ctcgagcagg atcgccgcga cgtcggtcgg cgcgacgagg ctgcggaaga
     3481 tgacgtgctc gatgtaatcg acgaccgtgt cgccgtagtc ggcgccttcg ggcgaagcga
     3541 ggatcggccg atacggatag cagtacggca cgtgcgtcac gccaggcatc gccggcgaga
     3601 agcctttctt ctgagtggcc ttgctcgcgg tgaacgccag cgatcccatc gagcggccgt
     3661 ggaacgcgcc gaggaagccg atgaagttcg ggcgggcggt gtgatagcgc gccagtttga
     3721 tcgccgcttc gaccgtctcg gtgccggagt tggtgaaaaa cacctggact tcttcttcca
     3781 tcgggcgcag gcggttgagc ttctccgcca actcgaccgt ctgcggcgtg tagaagtcgg
     3841 tcatcgcgta ctgaaggaac tgatcggcct ggcgttggat ggcttcgacg acggccgggt
     3901 gcgaatggcc ggtcgcggcc acggcgatcg tcgcgttcat gtcgatgtag cgcttgccat
     3961 ccacgtccca gacttccgag ccacggccgc gcgccatgac gaacgggtag gcgcgcgtga
     4021 gcgacggcga catcaccgcc cggtcgcgcg cgacccattc tcgcgccgtc aaaccggacg
     4081 gcgcagccat gtccggcgag gggtgcatca gggtagccat gttgtcctcc ttgtttgaaa
     4141 tcagcggtga cgacggaaac gaaggaggcc gcgtttgcgt tttcccggaa cgttcgacca
     4201 atgcggttgt ggcttgggga gaactgcaaa gggccgcgac cggagagggc gcgacattac
     4261 tcgtcggcgg tggaccactt ggcacggccc gcgttttcaa gcggaattta gtgggacaga
     4321 gagatcaggc tgctcattta gggtcgaatc gttatcgtcg ccaggtcgat cgcatctcca
     4381 ccggtggaaa gcggcagccg ccccgcgtca tcgtacaacc ccactcgaag acggtagtct
     4441 ccaggcggca ggtcgggcgg cagtcgtatg ctgaatcggt tgcgcattct cgcgccggcc
     4501 ggccacagcg atgtggggta cgaatccgcg ccggcaatac cgtccgcctg cgctgcaatc
     4561 gatccgtctg ggctgaccag gtgcgtgaag gcatggtaat tgcgcgcgat cagcccgctt
     4621 gactcaaagt atagcgccaa atccagagcc tcgccaggga gagatcggtt tgaattcagg
     4681 tcgaatccga ccaggcggat aggctcctca aactctacag cgacaggctg ctgcggctcg
     4741 gcctgaaact cggcgtcgcg caagtcgaag agcaagagcg acatttcatg gaaattccgc
     4801 ccgacttcga ggtggtcggc ctgttcctga agctcgctca ggatgacttc cgtcggatcc
     4861 gcgatgtttt gctgccacaa gaccagccaa gcgcgcggat gtttcatggc aaggtccgca
     4921 agccgggtga cggcacgcgt gtcgagcggt gattgtgcgg ccggtaacaa atcgggcgga
     4981 agcgggatga catctgcgct gccgcgataa tagtagtcga agacgggcgc ttgatgcccg
     5041 ccgacgagca ggatcgcatc gcctgatagt tcgtggtcct cgatataggc ggcgacgctg
     5101 cgcacatcgg gccgggccac agatgggtct aactggattt gggcggccac gagcagatcg
     5161 agcgcgagga tcgacagcaa aagtatcgcc gcgaggacga aaagcaggcg gttacgaaaa
     5221 cgcagcagcg catcgatgcc gagcgcggcg agcgtgacga aagccggcag cgacggcagc
     5281 aagtagcgcg gggcgaactt gggtcgctcc caggcgagga tagacatcag gaccagcggc
     5341 acgaccagta ggaagacgag tacagggtca gagtgagagt tagagtgggg agtggtggcg
     5401 cggcggatga atcggctggc gaggccggcc aggatgatcg ccagccacag cacgctgacc
     5461 agatgggaca ggtctgggag tgccggggaa ggcagctcgc cagcggtaaa tgcgcgccac
     5521 gtatcgaaca caaccgtatc ccacgtcacg cgaccgggga aataggtggc gttcgaggcg
     5581 gcctgggcca gggcgttggg cagccagggg aggaagagaa cagcaatggc aagctgggcc
     5641 aggagccagg cgcgtatctt gtgctgcgtg ctgcgtgttg cgtgttgcgt gtcgcgtgct
     5701 gcgtgtggct tgtggggcgt cgagctgtcg ccggaacacg gaacacgcaa cacggaatac
     5761 ctatctggca atttgcttct ggcgaggtgc accaggaacg ccaggttgat cgccactatc
     5821 gggaatgcag cgaagtagtg cgtgtacagc gccgccgtgc atgagagaat gtaagccagc
     5881 cacacacggc gacgccaatc cccaataacc catcttaaaa aagccagcgc ggcgatcaga
     5941 ataagagtaa tgagtaatga gtaaccgcgc gcctcctgcc cgtaccatac cgccatcggc
     6001 gcgacggcca tcagcccggc gccgatcagc cccacccgat gtccccccac cctccggccc
     6061 agcgcatgcg ccagcgcgac ggcgagcgtc ccgaagaaga ccgatgcgaa tcgaatcgag
     6121 aactcactcg atccggcagc gcgcatccac acgttgagca gcagggcgtg caggggtgga
     6181 tgatcggtga tggcgctttg ggcaatcgtc tcgccgggtg gcagcgcagc gaggtaggcg
     6241 ctgagcccct cgtcgtacca cagagactgc gcgtcgagac gatagacgcg cagcgcgaaa
     6301 gcgaggagga gaatgaaaag cgcggccagg ctgatgcggc gaacgaacgt ggcgttttcg
     6361 gcggtcatca ctggtgcgga agtataccat gaaccctttg gaaactggcg aagtgatgtt
     6421 tgtcatcgag gcttgtgaca gattccacta aggcccgtgg gttgcccata gtattatgct
     6481 tgtgggtcat tcgccccact ccgtggggtg tttgccctac ttctgtgtca taaatatgaa
     6541 tcgctgagcc atgttgacgg gtgcaggcta agcgaattgt tgttgtctct cgagttagcc
     6601 cgcatcgaaa ggagtcgcct atcgaggaag cacggttgag taacccgtga gcgttgatgt
     6661 gactccatcg agaggagaaa gccatatgcg acataacaag ttcatatggt ttggcgtggt
     6721 cgcagtcgtg gcgctggtgc tggcgggctg cgccggcccg cagggtgagc aggggccacc
     6781 cggtccagcc gggccgcccg gaccggaagg cccacagggg ccgccagggc cggcaggagc
     6841 ggcgggcgaa gcggcggccg ccgcagaggt cggcgccgaa tacgtcggca gccagacctg
     6901 cggccaatgc cacgagacaa tctacgagac gttcatgaag tccggccacc cgtataagct
     6961 gaacaaggtc gtcgacggca agccgccggt gtacccgttc accgagctgc cctcgccgcc
     7021 cgaaggctac acctgggacg acatcagcta cgtcatcggc gggtacggct ggaaggcccg
     7081 cttcatggat caacagggct acatcatcac cgacaagccg ggcgcgacga tcagcgatac
     7141 gaactatctc aaccagtaca actacgccaa cgcggtcgtc ggcgcggacg ccggttgggt
     7201 gaagtacaag tcaggcacac ctcagttgaa gtacgactgt ggcacgtgcc acacgaccgg
     7261 ctactcggcg tttccgcccg attcgcacca ggacgacctg cccggcatca tcggcacgtg
     7321 ggccgcgccg ggcatccagt gcgagcgctg ccatggccca ggcggcaacc acgtgtccga
     7381 tccgggcggc gtgagcatgg aaatcgagcg cagctcggcg ctgtgcggcg actgccacat
     7441 ccgtggcgcc gtcgagtcgg tcaacgcggc aggcggcttc atcgagcacc acgagcagta
     7501 cgaggagctg ttccagagca agcacatcac cctcgactgc gtgatctgcc acgatccgca
     7561 cacgctcgtg atccaactgc gcaaggcgca ggagtctgac ccgtcggtgc agacgacgcg
     7621 cacacaatgc cagaactgcc acttcaagca ggcccagtac caagattcgg aggtccaccc
     7681 ggcgatcggc gtgcaatgta ccgactgcca catgccgcgc atcgccaaga cggcctgggg
     7741 cgacgcggag aagttcaccg gcgacatccg cacgcacctg atggcgatcg atccgaaaca
     7801 gatcgggcag ttctcggagg acggcacgac ggcgctgtcg cagatcgggc tggacttcgc
     7861 ctgccggcag tgccacgtcg aaggcggcaa ggcgacgccc aagaccgacg agcagttgat
     7921 cgacaaggcg accggctacc atacgccgcc gcaagagtag caccactgtc gaagcacagg
     7981 cgcgggtcga gcttccgtcg gcccgcgttt gtccagcatg ccatacatgt ttataatgtc
     8041 ggccatccca ctacccgcct ggagggaatt ccttgtctaa aagacggctg accatcgggg
     8101 ttggcctggc cggcctggcg ctcgcggcac tctcgttgag cgccgggggc gcttcggcga
     8161 cgccggcagc gcctgcaccg ctcgcgcagg gagtggacaa cgagacctgc ctggcctgtc
     8221 acactcaacc cgacatgcgc acgacgctgc cctcgggcga agagctgttc ttgacgatcg
     8281 accgcgatgt gtacaacagt tccgtgcacg gcgaagacgg ctacgcctgc gtgcagtgcc
     8341 atatcggata caccggattc ccgcacgagc cgatccaggt gcagacccgg cgtgagttca
     8401 gcgtgcgaca ggccgcctcg tgcagccgct gccaccagca gcagcacgag cagacactcg
     8461 tgaatgctca cggccaggcg caggcggcgg gcaaggtcga agccgccgtg tgcaccgact
     8521 gccacggcgc gcacaacgtc gagtcgttcg cggagaggcc gcgcagcgcc atcccgcaga
     8581 cgtgccagaa gtgtcactcc gagatctacg cgctctacga ggacagcgtg cacggcgcgg
     8641 cgctgatcgg cgaaggcaac cccgacgtgc cggcctgcac cgattgccac ggcacgcaca
     8701 ctgtagaggg gccttccagc gtcgagcgct tccgccttca ctcgccggaa atctgcgcca
     8761 agtgtcacgc ggacaaagag ttgatggcga agtacggcat cagcactgag gtgttcgaga
     8821 catatctgtc cgatttccac ggcaagacgg tcctcttcca ggcatccgtc ccgggccagg
     8881 agactaacaa gccggtgtgt atcgactgcc acagcgtgca caacatcctc ccgccggctg
     8941 atccggccag cacggtcaac atccagcgga acctgctgac gacttgccag aagtgccacc
     9001 cgagcgcgca ggagaacttc cccgcggcct ggcttggcca ctacgcgccg agccgcgaga
     9061 atgcgccgct cgtgtacttc gtcgatctgt tctacaagat cttcattccg agcgtgctgg
     9121 gtgtgatggc ggtgttcgtc gtcactgatg ccgcacggcg catcctcaat cggcgcaaga
     9181 ggcagggtca tgagtgaact tcgcaaatac ctccgctttc ggcgtccaca ccgcgtcgag
     9241 cactgggtgt tcatggccag tttcacgacg ctggccgtga ccggcctggc gcagaagtac
     9301 gcggaggctc ctatttcgca atggattgtg gtcacgctgg gcggcatcga gagcgtgcgc
     9361 gtcatccatc gcctcgccgc gatcacgata atggtcgtcg ccgtgtacca tatcggcgcg
     9421 gtaggctacc ggctttacgt gaatcgcagc cggctgcaca tgctgccggg cctgttcgac
     9481 gtgcgcaacg cgtggcagtc gctgaaatac aatctgggcc tgagcaaagc gcgcgtgcag
     9541 caaggccgct acggcttcga agagaaattg gaatactggg ccgtggtgtg gggcacgctg
     9601 atcatggtgc tcaccggctt tatgctgtgg aacccgatcg ccacgacgag catcctgccc
     9661 ggcgaggtcg ttcccgccgc gaagatcgcg cacggcctgg aagcggtgct tgccgtggtg
     9721 gccatcttcc tgtggcactt gtaccacgtg ctgatcagga cattcaaccg cagcatgttc
     9781 accggctacg tcaacgaaga gcagatgctc gacgagcacc cgctcgagct ggccgacatc
     9841 aaggccggca tcgccgagcg gccggtcgat ccgcagaaga aggagaggcg gcgcgtgttc
     9901 ttcggcgtgt acggcgtggt cgggaccgtg cttctggtgg gtatcttcgt cttcgttacg
     9961 ttcgagaaga cggcgatcga gacggtgccg gcgccgccgc cggagcaggt gacggtgttc
    10021 gcgccgctca caccgacgcc gctgccgacg cggctgccga ccgccacccc cgcgccggtc
    10081 acggcggtga cctgggcggg cggccgtggc gatctgttca aggcgaagtg cacggcatgc
    10141 cacggcagtc cgtcgggact gggcgggctg gatctcagca cgtacgaggg cgcactgaag
    10201 ggcggcaact ccggcctggg catcgtgccc ggcgatcccg aggcgagcgg catcgtccgg
    10261 attcagtccc aaggcgggca tcccggccag ctcaccgact cggagctgga gaccgtgatg
    10321 cagtggatcg aagccggcgc gccggagcaa tagacaactt ccctctccta cagggagagg
    10381 gatcaggggt gagagccgag gagaaatcat gaagcgaatc aagcgagcgt tacgcaggtt
    10441 ctttctaccc tcaccaggca gcccattgtg gcgtcgcgtc ctgccgtggg ccacgctcgc
    10501 cggtgtcttc gtgctggtgt cgttctccgg cgcttacgcc tgggaataca cgaactcgcc
    10561 ccagttctgc ggcacgacct gccatacgat gccgcccgaa tatacggcgt atctgacctc
    10621 accgcacgcc cgcatcctgt gcgtcgaatg tcacatcggg cgcgactacc tcggcagccg
    10681 cgtcttgcgc aaggcgggtg acctcaaaca catcgccgcc acgatcttta cggactacga
    10741 atttccgatc cgcgcagaag acatgcgccc ggcgcgcgag acgtgcgagc aatgccattc
    10801 accggacaag tttgcggacg acagcctgcg tgagatcagg cacttcggcg acgataagaa
    10861 caacacaccg gagagcatat ttctcacctt gaagaccggc ggcggcagca agcgccaggg
    10921 cctgggacgc ggcatccatt ggcacgtcga gaacaaggtc ttctacctgc cggccgatcc
    10981 gcacgagcag gacatcccct atgtgcgcgt ggtcggcgac gacgggcagg tcgccgaatt
    11041 tgtcgacgtc gagtccggcc ttgacccgaa tacgatcgac gaatcgaaac tggaagagat
    11101 ggattgcatc acatgccaca accgcatcac gcacctgatc ctgccgccgg aggaacgggt
    11161 cgatcagttg ctggcgcgtc aggtcattcc ctcgaccatc ccggacatcc gccgccaggc
    11221 cgtcgctgcc ctgggcgctc cgtacgaatc catggcgctg gccatgaacg gcatcgaggg
    11281 cctgcgcggc tactaccaga gcgtgtatcc cgagttctac accgcaaacc aggatcaggt
    11341 cgaagccgtg atcgccacgc ttcaggagac ctaccggaac agtgtatttc cggagcagaa
    11401 agccgactgg acgacacacg cgaacaacat cggccacaag tcgtcgcccg gctgcttccg
    11461 ctgccacgac ggcaaacatc tgacctcgga aggtgagtcg atccggctgg agtgcaacct
    11521 gtgccactcg atccccgtcg tggccggcgc cgacgacttc gtcgtggaca tcgagatcag
    11581 ccgcggcccc gagcctgaat cgcacttgaa ctccaactgg atcgcgctgc atcgtgacgc
    11641 ttttgacacc acgtgcgcca actgccacac gaccgaagat ccaggcggcg tgagcgacac
    11701 gtcgttctgc tcgaatagcg gctgccacgg tcagacctgg gagtacgctg gcttcgatgc
    11761 gccggcgctg cgagcgaagt tacagggcca gcttcctccc acacccacgc cgatgccgac
    11821 gccagccggc ggtcttccgc tgacgtacga ggccgccatc ggcccgctgt tccaggggcg
    11881 ctgcgcggcc tgtcacgggg aggatgggat tcagggtctg gacttgacga cgtacgaggg
    11941 cgtgatggcc ggcagccaga acgggccggt cgtcgttccg ggcgatgccg aggccagtct
    12001 cttggtggag aaacaatcgg gcgcgcagcc gcacttcggc cagctcacgc cggacgaatt
    12061 gcaactggtg atcgagtgga ttgaggccgg tgcgcccgag aagtgaaccg cgtgtcactg
    12121 attactgatt actgattact aattactgat cactgaccac tgaccactga tgaccgcctc
    12181 cgccgtctct ctacacagag gtctgcgcgc caagatcgcg ctgggcgtcg ccctgccgat
    12241 cttcgtcgtg ctgctctcgc tgtcgctgtt ccacatctgg cgcgagcggc acctggccga
    12301 gacgcagatc gagtcggcgg cgctgcagat gggcgagatg acgctgggcg gcctgcgcca
    12361 cgcgatggcg accggcgacg atgcggcgct caagcagatc ctgtccgacg tgagccggct
    12421 ggacaacata caccaggtgc agatcatcgg cctcgatggg cgggtgaagg ccaacagtct
    12481 cgggcggcgc gtcggcgagg ttcagaatct cgaccagtcc ggctgtgtcg aatgccatcc
    12541 ggcatccggc gaagcgacag cgcggactgc ccggctcccc tacccgtctg ggattatgcg
    12601 cgtgtccctg ccgattcgga acgaagcgga ctgcgccggc tgccataccc cgctcaacgc
    12661 cttgctcggt acgctgctgg tcgacgtctc ggtcaccgat ttggaaaaac atctgctgga
    12721 cgatcttcgc gtggacctgc tgctgacgag cgggatcacg gttctcgcga cgctcggcat
    12781 ctacgtcctg atccatcgcc tggtcgtgcg ccgcatcggg gccatgcaga agccgctcgc
    12841 ccggtttgcg caagggagct tcagcgcgcg catgcctgtc tcgctggatc ccccggacga
    12901 gatcgacgcg ctggccgatg ccttcaacca catggccgac gagctgcagc gccacacccg
    12961 cgagcaggaa gagcgcagcc aggtgcgcca gcgcgcgatt gtcgaggagc gcgagcgcat
    13021 cgcgcgcgag ctgcacgacg gcatggcgca ggtgctgggt tacgtcagca ccaaggtgat
    13081 ggccgcgcgt ctgatgctga agaagcagcg agcgcatgag gccgagcagc atctactcca
    13141 actcgaagag gcggcccagc ggctgttcgt cgacgtgcgc gaagcgatcc tcggcctgaa
    13201 gatggctgcc cggagcggcg caggactacc gtcggccctt cgcgagtaca ccgctcaata
    13261 tcatcaactc accggcatcc cggtcgatct gtcgattgac ccggccgccg aggacctggc
    13321 gctgcccgca gagactgaac tgcagatggt gcgcatcgtg caggaggcgc tgaccaacgt
    13381 gcgcaagcac gcgtcggcca aacgcgcgtc cgtggggctg caccgcgaaa acggcacgct
    13441 ggagttgctt gtgcgcgacg acggcgtcgg gttcgatccg gatggggcgc gcttgcgcga
    13501 tagtggccag ccgcactttg gactcggcac gatgcgcgaa cgtgcggacg caatcggcgc
    13561 ggacttcaac cttcattccg agccaggcgc cggcacgcag gtcagcgtgc gcttgaagat
    13621 acagggatag ggaagtggca tctgtgcgtg tcttggttgc cgatgaccat tccttgtttc
    13681 gtgatggcat cgtcagcctg ctcgaagcgg ccggcttcca ggtgatcggg caggtcggcg
    13741 acgggcgggc ggccgtggaa gccgcgcggc gcctgcatcc cgacctggtg ctgatggacc
    13801 tgtccatgcc ggagatgacg gggcttgagg cgctgcgcgc gatccgcgcg gagatgccag
    13861 acgtccaggt cgtgatgctg accgtatccg acgaagacag cgatctcttc gaggccatcc
    13921 gtgccggcgc gcgtggttac ctgctcaaaa gcttgaacgc ggacgagttc atcgagcttc
    13981 tggaaggctt gcagcgcggc gacgccgcta tcagccggaa gacggcgact cgcctcatcg
    14041 acggatatgc agcgctctcc cggcagcaaa cgaaaccctc cgagagcctg ctgacgccgc
    14101 gcgaaatgga gctgctccaa ctgatcgtcg atgggatgtc gaacaaggct atcgccgagc
    14161 ggctatcgct cagcgagaac acaatcaagt accacatcaa gaacattctg cagaaactag
    14221 gtgtccagaa ccggaccgaa gccgcgacac aggcgctgcg cagcggcttg ctcagcccga
    14281 ccaggcatgt cgacccatga gccgatgcaa tgccgcactc ggtcaaaacg ccatagttgg
    14341 ctgtggccaa actccagaag gagaggaaag acaggcatgg acgaagagcg aaaaatcgga
    14401 cgtcggaagt ttctgagcct cacgacctgg gcgatcggcg gggtgatcag cgccg
//
LOCUS       DBNHXB010000421         8774 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_462493, whole genome shotgun sequence.
ACCESSION   DBNHXB010000421 DBNHXB010000000
VERSION     DBNHXB010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8774)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8774
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_462493"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="AC162G_16660"
     CDS             <1..1605
                     /locus_tag="AC162G_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077490.1"
                     /translation="IMLKIFPIVVVPIALRRTTTPRQVAGYLLALAVTIGAILLPFLL
                     ARADFVLAFVQSTLNRPTWLSIWALFDGNYLYGVAEPIIERFTTENIGAPPNSTLPWP
                     LIHAGFLVLYLVIYTRRVDWREPVKSIALGGLTVNLFLLWSKGFSGQFISYAFPFLIL
                     LMPDLRGVVYTLLLSVLWVAEWPIAFNMLDGQNGFIVWIVIARTAVLVALCLEYAARL
                     FSATAETRLAGFAQAAPRLASTLLVAGWISVIPAGVLAVHTYTETRLAADSAAPALEA
                     ILSGSTSNSDIIAIAQPRLFRRLYPHARLIGEPTLLPVFRHTPEEARLAWLNEIAARG
                     PFWFIADESDPETLEENRRAEKWLSENACKIVTTLAGTARLSRFMGASDAPVEVAVEA
                     TFADEIELSGARLSSRSLHPGDGLCVEISWQAIETPSSDYTVFVHLIDAQGRLVAQND
                     QQPVGEFAATSGWATGSTIVDRHGILLPADLSPGEYVLRVGLYRSDDLSPVPFMQNGA
                     PSPDTSGMTLAPITLASTLPAPERPQ"
     gene            1602..3965
                     /locus_tag="AC162G_16665"
     CDS             1602..3965
                     /locus_tag="AC162G_16665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR5077491.1"
                     /translation="MKYRFTAVLLLVMLAWLELSTLAVTTPTVDEPLHIMRGYAFVAR
                     DEERYRMYGPVLADALSGIPLLLEPDLQFPPADDPFWLGVANMEHADKLIRANDASLQ
                     RIFFLARFPIIAVSLLLGAFIFRWARERSGAPPALGALLLYVFCPNLLAHSRLAATDA
                     ATASTFLISAYAFSRSLESPRFMTRAASGAALGLALATKFSAVALLVAFIIEATLRAL
                     RDRRRAAVAAIAGTLGVTVAIGALTVWAVYRFRVDSLETGGVPVPAPYYWREWQALNE
                     YLQDPLPGYLFGELSPRGWWYYYPIAFLVKTPLPLQLFLLLSVAYTLRRRVWIRDMSL
                     YLVPGLFFAALLFSPHALGYRYLLPVLPFIFVLSADVIAAAQRMRWSQFAIGALLIWQ
                     VWGTLRIYPYYLPFFNEIVGGSDRGRYILSDSNIDWGQDLIGLKYYVDHNHLERINLS
                     YFGVIHPTAYGLNAEPLPPVLAAMQDQGGWQLRMLYPSDPAPGTYAISVMNLMGNLAT
                     DRSVYAFFRDRKPDAIIGGSIYVYTVSAHGEPTDLMLSGLQVDRIDPETFSRLGTNDV
                     RLRWSDASAALIAPPGYSWIAIADRTAPAENFARFFDGIARQVRTQTTGGRSYNLYRL
                     DLGDRILEAAASAERSVAWNAEVHPDPTATPTAELPAQFGEVLELLGYTLTTDTPSGE
                     AHLLTYWRVLSPISTPLWAFAHAVGQQAQIVAQEDRWDAAPKGWRPGDLIVQLHRLAI
                     PPGSGPVWIEVGLHNPESGERLPAIVDGREVDRRLLLKYLTPQDAPP"
     gene            3962..6016
                     /locus_tag="AC162G_16670"
     CDS             3962..6016
                     /locus_tag="AC162G_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077492.1"
                     /translation="MKRSLICEILISLAVFAVVFGVYITSGNNVPFDSRWSIHTAMSI
                     IREGNTDLDEYRELAEPREEYQNSLETVDGHLYTIYPIGASLLAVPFVFVLDRGFELS
                     VAFDLNTYVAHTIPAEIEALVASVYVALTAVIFYWIARRFLKRPASLLVVFIFAFCTP
                     AWSTASRALWQHGPSMLLLATALLIIVYAKDHAQRIQYASIPLAASVVVRPVNIVSAA
                     LLTLFVFTEHRRHFGRFLLWALPVAALFTAYNLSVYHSLLPGYYLGASRGSNDFSVST
                     LPEALVGHWFSPARGLLVFSPVLVFSVAGLIFKLRRQRFERLDLFLIEAVVAQWIVMS
                     VWPIWWGGWSFGPRLMSDVVPYLVYWIIPALEWIGTLVGWRRTAGIVTLLATIAVSFF
                     IHYRGANAFDTIDDWNRHPANIDTRSGRLWDWRDIQFLRGLKWGVPAHVSASGVPIKQ
                     LDPDTYLRLGTNDLRVRKFEAGTSFIAPSGESWLILADGTSNAPELAFVTEGAALADR
                     TRTFTDRIPYRVYRDDFGARILTTAQRAEQVAAWAVDIVPDSTTVLPVTLPAAFGQSA
                     ELLAFEVVTDGLSSRVTVTTYWRAGEALAPPQKLFVHALNAQAQIVGQRDGFDAPMRD
                     WSPGDLIVQVSQMTVPPDSGPIWIEAGLFNPDTGSRLPVTVGLETVTDRLLLERVEIE
                     SR"
     gene            6197..6367
                     /locus_tag="AC162G_16675"
     CDS             6197..6367
                     /locus_tag="AC162G_16675"
                     /inference="COORDINATES: protein motif:HMM:NF021570.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HIR5077493.1"
                     /translation="MHQAPNPNTDSSKTVTEFPQFSFLLGDLHPHTLNLPFVLPAIGL
                     AVNAYQPRTTAR"
     gene            complement(6411..8456)
                     /locus_tag="AC162G_16680"
     CDS             complement(6411..8456)
                     /locus_tag="AC162G_16680"
                     /inference="COORDINATES: protein motif:HMM:NF022481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-mannosyl-transferase family"
                     /protein_id="HIR5077494.1"
                     /translation="MLASDWRKAKRHGWLFDSTAAGSGCLRVVCETLVAKGVGLMVSS
                     IARSLSRFELKLSPRMAVIGAAILALGLFLTTLQLDVNGSSHPYATDVGEIQNALPRW
                     GTIHFTGYPLYTAIGSAFVAALRVVGLPPAAGASLYSATWGAISIGLLTSLILAFGVQ
                     PVIAASSAVLFGLTTSMWTFASVAEVHTMTMALTFATVLVALRYGREGRTADLYLLAF
                     LVSQGVAHQRAFVFMGLGLLVFVARQMPQWRIVVRKLPAITGLALLGPLTYLYLPMRA
                     WQGADWVFSSPGTWNGFWALFLDTKADRIVEIPESLAELGSRIQGVFELLTADMPWLL
                     LVLGLAGLLLPMRRVKWVERFGLMLLGATYIALSLIIWIGRVGEAVLATKLSVVAIAI
                     IGLALLAQALWDRRPLFGQLAAAALIAAVAFLYLRNSPPVLAVTRDPSAQETIQLVAS
                     VPPAEDGRPSAIMALWGNDYWQLAYAQTFQNQFPEIRLLKHDRNFGNVLNQGTHLLTL
                     SYTFYLRPPEWWEQLLGISVRLSSRAYGIVEISDRPSMSSADATSSEPFPLGNGIAIR
                     HAELSWASKDTLRMTVEWQSQQDHLADYSVAVHLISQDPPTDPQHIIAQADQAHPVYG
                     WYPTARWVNGEIVRDDYSLSVPPDSDPVAVRIGMYQVLADGSFQNSNWLSLPVPETP"
     gene            complement(8640..8762)
                     /locus_tag="AC162G_16685"
     CDS             complement(8640..8762)
                     /locus_tag="AC162G_16685"
                     /inference="COORDINATES: protein motif:HMM:NF017494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HIR5077495.1"
                     /translation="MWVIEVKDFPAVVTMDAHGASLHAEIEAKSKGVLEQLIAA"
BASE COUNT         1633 a         2731 c         2579 g         1831 t
ORIGIN      
        1 atcatgctca agattttccc gattgtcgtc gtgcccattg cactgcggcg cacgacgacc
       61 cctcggcagg tggctggcta tctgctcgcg ctcgccgtga ccatcggggc aatcctcttg
      121 ccattcctgc tcgcccgggc cgatttcgtt ttggccttcg ttcaatcgac gctgaatcgg
      181 ccgacctggc tatcgatctg ggccttgttc gatggcaact acctgtacgg cgtcgccgaa
      241 ccgatcatcg agcgcttcac aaccgagaac atcggcgcgc caccgaacag cacgctcccc
      301 tggccgctca tccatgccgg ttttctagtc ctctatctgg ttatctacac gcgacgcgtt
      361 gactggcggg aaccggtcaa atcgatcgcg ttgggcggct tgacagtgaa cctcttcctg
      421 ctgtggtcga aagggttctc gggacagttc atttcctacg ccttcccttt cctcattctg
      481 ctcatgcccg atctgcgcgg cgtggtgtat accttgctat tgagcgtgct gtgggtggcg
      541 gagtggccga tcgcgtttaa catgctggac gggcagaacg ggttcatcgt gtggatcgtg
      601 atcgcgcgca cagccgttct cgtcgcgctg tgcctcgaat acgcggcgcg actcttctca
      661 gcgacggccg agactcgact tgctggcttc gcgcaggcag cgccacgcct cgcctctacc
      721 ctgttggtgg cgggatggat cagcgtgata ccggccggcg tgctggcagt gcatacgtac
      781 accgaaacac ggctcgcggc cgattctgcg gcgccggcgc tcgaggcgat cctttccggc
      841 agcacgagca actcggatat catagccatc gcgcagccac ggctgttccg acggctgtac
      901 ccccatgcgc gactaattgg tgaaccgacg ctgctgcccg tattcaggca cacgccggag
      961 gaagcgcgcc tggcctggtt gaacgagatc gccgcgcgcg gtccgttttg gttcatcgcc
     1021 gacgaaagcg atcccgaaac gctcgaagag aaccgccgtg ccgagaagtg gttgtccgaa
     1081 aacgcctgca agatcgtgac tacgcttgcc ggcacggcgc gcctgtcgcg cttcatgggt
     1141 gcgagcgatg cgccggttga agtggccgtc gaagcgacat tcgccgacga gatcgagctg
     1201 agcggggcgc gtctcagcag ccggagcctg catcccggcg atgggctttg cgtggaaata
     1261 agttggcaag cgatcgaaac accttcatct gactacacgg tgtttgtcca tctcatcgat
     1321 gcacagggtc ggttggtcgc tcagaacgac cagcagccgg tgggcgaatt cgcggcgacg
     1381 agcgggtggg ccaccggatc gacgattgtg gacaggcacg gcatcctctt accggccgac
     1441 ctctcgccgg gcgagtacgt cttgcgcgtt ggtctatacc gctcggacga cttgtcgcct
     1501 gtgcccttta tgcagaatgg ggctccatcg ccagacacga gcgggatgac tttggcgccc
     1561 atcactctag cctcaaccct tccagcgccc gagagacccc aatgaagtat cgtttcactg
     1621 ccgtcctgct gttggtgatg ctggcctggc tggagttgtc gacgctggcg gtgaccacgc
     1681 ccaccgtaga cgagccactg catatcatgc gcggatacgc gttcgtcgcg cgggatgagg
     1741 agcgctatcg catgtacggg ccggtcctgg ccgatgccct gagcggcatt cctttgctgc
     1801 tggaacccga tctgcagttc ccacccgccg acgatccgtt ctggctcggt gtggccaaca
     1861 tggaacatgc cgacaagctg atccgggcca atgacgcctc tttgcagcgg atcttctttc
     1921 tggcccgatt tccgatcatt gccgtcagct tgttgctggg cgcgttcatc tttcgctggg
     1981 cccgcgagcg cagcggcgca cctccggcat tgggcgcgct gctgctctac gtattttgcc
     2041 ccaacctgct ggcccattcg cggctagcgg cgacggacgc ggctacggcg tcaaccttct
     2101 taatcagcgc atacgccttc agccggtcgc tcgaatcacc ccgcttcatg acccgcgcag
     2161 cgagcggcgc ggcgctcggc ctggcactag cgaccaagtt ctcggcagtc gcgctgctcg
     2221 tcgccttcat catcgaagcg acactccgcg ccctgcgcga ccgtcgccgc gcggcggtgg
     2281 cggccattgc aggcacgctc ggcgtcacgg ttgcgatcgg cgcgctgacg gtatgggccg
     2341 tttaccggtt ccgggtcgac tcgctcgaga cgggcggcgt tccggtgcct gctccctact
     2401 actggcgcga atggcaggcg ctgaacgaat acctgcaaga cccactgcct ggctacctgt
     2461 tcggcgagct gtcgccgcgc ggctggtggt actattaccc gatcgcgttt ctagtcaaga
     2521 caccactgcc gctgcaactg ttcctgctct tatccgtggc gtacacgttg cgccggcgcg
     2581 tctggatcag ggacatgtcg ttgtatctcg tgccgggcct gttctttgcc gccctcttgt
     2641 tcagccctca tgctctgggt tatcgctacc tgttgccggt actgcccttc atctttgtcc
     2701 tcagtgccga cgtcatcgcc gcggcacagc ggatgcgttg gtcacagttt gccatcggcg
     2761 ctttgctcat ctggcaagtc tggggcacgc tgaggattta tccatactat ctgccgttct
     2821 tcaatgaaat cgtcggcgga tccgatcggg gtcgctacat tctctcggat tccaacatcg
     2881 attgggggca ggatttgatc gggttgaaat actacgtcga tcacaaccat cttgagagga
     2941 tcaatctgag ttacttcggg gtcatccatc cgacagccta cggtctgaac gccgaacctt
     3001 tgccgcctgt gctggccgcc atgcaggatc aagggggatg gcagttgcga atgctctatc
     3061 cgtcagaccc ggctcccggc acctacgcga tcagcgtgat gaatttgatg ggcaatctcg
     3121 caaccgatcg aagcgtgtat gccttcttcc gcgatcggaa acccgacgcc atcatcggcg
     3181 gctcgatcta cgtgtacacc gtttccgcgc acggcgagcc gaccgatcta atgctgtccg
     3241 gtctccaagt cgatcgaatc gacccggaga ctttcagccg cctgggtaca aatgatgtgc
     3301 ggttacgctg gtccgatgcg tctgccgcgt tgatcgcgcc acctggctat tcgtggatag
     3361 ccattgccga tcggacagcg cctgcggaga attttgcccg atttttcgac gggatagctc
     3421 gccaagtgcg cacgcagaca accggcgggc gttcatacaa tctatatcgc cttgatctgg
     3481 gcgatcgcat tttggaagct gccgcgagcg ccgagcggtc ggtggcatgg aacgccgaag
     3541 tgcatccgga tccgacggcg acgcccaccg ccgaactgcc tgcgcagttc ggcgaagtgc
     3601 tcgaactgct cggatataca ctcacgaccg acacaccatc aggcgaggcg cacctgctga
     3661 cgtactggcg cgtcctcagt cccatctcga caccgctgtg ggcgttcgcc cacgcggtcg
     3721 gacaacaggc acagatcgtc gcgcaagaag atcgatggga tgccgcgccg aagggctggc
     3781 ggccgggtga tctcatcgtc cagcttcacc gcctggcgat cccgcccgga tctggccccg
     3841 tatggataga ggtcgggcta cacaatccgg aatccggcga acgcctgccg gcgatcgtcg
     3901 atgggcgcga ggtcgatcgg cgcctgctgc tcaaatacct cacccctcag gatgctccgc
     3961 cgtgaagcgc tctttaatct gcgagatact catttcgctg gccgttttcg cagtggtctt
     4021 cggcgtgtac ataaccagcg gaaacaacgt cccgtttgat tcgcgctggt caatccacac
     4081 ggctatgagc atcattcgag aggggaacac tgatctggac gagtaccgcg aactcgccga
     4141 accgcgagag gaataccaga actcgctcga aaccgtcgac ggccacctgt acacgatcta
     4201 ccctatcggc gcgtccctcc tggccgtgcc gtttgtcttc gtcctcgatc ggggtttcga
     4261 actgtctgtc gccttcgatc tgaacacgta cgttgcgcac acgatccctg ccgagatcga
     4321 ggcactggtc gcaagtgtct atgtcgcgtt aacggccgtt atcttttatt ggatcgcccg
     4381 ccgcttcctg aagcgacccg cgtcgctact ggtcgtcttc atctttgctt tttgcacgcc
     4441 ggcctggtcg acagcgagcc gcgcgctatg gcagcacggg ccgtcgatgt tgttgctggc
     4501 gacggcgctg ttgatcatcg tctacgcaaa ggatcacgcg cagcggatac agtacgccag
     4561 cattccactt gcggcttcgg tcgttgtgcg cccagtcaac atcgtgtcgg cggccttgct
     4621 gacactcttc gtgttcaccg agcatcgccg ccacttcggg cggttcctgc tatgggcgct
     4681 gccggtcgct gcgctcttca ccgcctacaa tctgtctgtc tatcattcct tgctgcccgg
     4741 ctactatctt ggagccagcc ggggaagcaa cgatttctcg gtatcgacgt tgccagaagc
     4801 gctcgtcggg cactggttta gcccggcacg aggattgctg gtcttctcgc cagtgctggt
     4861 gttctccgtt gccggcctga ttttcaagct caggcggcag cgattcgagc ggctagactt
     4921 gttcctgatc gaagccgtcg tcgcacaatg gatcgtcatg tcggtctggc cgatatggtg
     4981 gggcggctgg tcgttcggcc cgcgcctcat gtccgacgtg gttccatacc tggtctattg
     5041 gatcatcccg gcactggagt ggatcggcac actggtggga tggcggagaa cggccggcat
     5101 cgtcaccctg ctcgcgacca ttgcagtaag ctttttcatt cattatcggg gcgcaaacgc
     5161 ctttgacacg attgacgact ggaatcggca tccggccaac atcgacacca ggtcggggcg
     5221 attgtgggac tggcgggaca ttcagttctt gcgcggcctg aagtggggcg tgccggcaca
     5281 cgtgtcggca tcgggagtgc cgatcaagca gctcgatccg gacacttatc tgcgccttgg
     5341 cacgaacgac ctgcgcgtcc gcaaattcga agccgggaca tcgttcatcg ctccgtcagg
     5401 tgaatcgtgg ctcattctcg ccgacggtac gtcgaacgct ccagaactcg ccttcgtcac
     5461 ggagggggca gctctggcgg acaggacacg gacctttacc gatcggatac cctatcgggt
     5521 ctatcgcgat gacttcggcg cgcgaattct cacgacagca caacgagccg agcaggtcgc
     5581 cgcctgggct gtggacattg tgccggactc gactacggtc ctgccggtca cattgcccgc
     5641 cgcctttgga caatctgcgg aactgctggc attcgaggtc gtgaccgacg gtctatccag
     5701 tcgtgtgacg gtgacgacct actggcgcgc tggcgaagcg ctcgcgccgc ctcagaagct
     5761 atttgtccac gcgctcaatg cacaggccca gattgtcggg caacgcgatg gatttgatgc
     5821 gcccatgagg gattggtccc caggcgatct gatcgtgcag gtcagtcaaa tgactgtccc
     5881 acctgattcc gggccgatct ggattgaagc cgggttgttc aatccggata caggttcgcg
     5941 gctgcctgtg accgtcggcc ttgaaacggt gaccgatcgg ctcctgcttg agcgtgtcga
     6001 gatcgaatcc cggtgatgcc gcacgcacct cagaaacacg gagacacgga actaccgtgg
     6061 caggtccgtg cctccatgct catcaacgca gacagtcagt tcatgccgat cgtgttatgt
     6121 cgcgattagt tgctccagca cctgcttcga actggcctac ccgccggatg ccgtcagccc
     6181 aagtctggcg accgtgttgc atcaagctcc taatcccaac accgactcct ccaagaccgt
     6241 caccgagttc ccgcaattca gtttcttgct gggcgatctg catccacaca cccttaacct
     6301 gccattcgtg ttgccggcca tcggcctggc ggtgaatgca tatcagccgc gcacgacggc
     6361 aagatgatgt ccgattgggg tgagcggtgg cggctgacac cgcgtctcgc ttatggtgtc
     6421 tcgggcactg gcagcgaaag ccaattactg ttctggaatg acccgtctgc aaggacttgg
     6481 tacatgccaa ttcggaccgc aaccgggtca gaatccggcg gcacgcttag tgaatagtcg
     6541 tcgcgcacga tttctccgtt cacccagcgg gcggttggat accatccata gaccggatga
     6601 gcctgatcgg cttgcgcaat gatgtgctgt ggatccgtgg gcggatcttg agagatcaaa
     6661 tgcacggcga cgctatagtc ggcgaggtga tcttgctgcg attgccattc gacggtcatc
     6721 cgtaaagtgt ctttggacgc ccatgacaac tcggcgtgcc gaatcgctat cccgtttccc
     6781 agcgggaacg gctcggaaga ggtcgcatct gcagacgaca tggagggtcg gtcggaaatc
     6841 tcgacgatcc cgtaagccct ggatgacaag cgtacggaga tgccgagtag ttgttcccac
     6901 cattctggcg gccgcaggta gaaagtgtag cttagcgtca gcaaatgggt tccttgattc
     6961 aagacgttac cgaagtttct atcatgcttt aatagcctga tctcgggaaa ctgattttga
     7021 aaagtctgcg cataggccag ttgccagtaa tcatttcccc acagcgccat gattgcgctc
     7081 gggcgtccgt cctcagcggg agggacgctg gcaaccaact gtattgtttc ttgagcactc
     7141 gggtcacgcg tcaccgccag aaccggaggg ctgttccgaa ggtataaaaa ggcgactgcg
     7201 gcgatgagcg ccgccgccgc cagttgcccg aacagcggcc tgcgatccca cagggcttgc
     7261 gcaagaagcg ccaggccgat gatcgctatc gccaccacgg acagtttggt tgccaggacg
     7321 gcctcgccga cacgcccgat ccagatgatg aggctcaggg cgatgtaggt cgctccaagc
     7381 agcatcaggc cgaatctctc gacccacttc actcgccgca tcggcagcaa caggcccgct
     7441 agcccgagta ccaatagaag ccacggcatg tcagcggtca gcaattcaaa cacaccctgt
     7501 atccgtgaac ccagttcagc cagcgattct ggtatctcga caattcgatc ggctttggtg
     7561 tcgaggaata aggcccaaaa gccattccat gtacccggcg agctgaagac ccagtccgct
     7621 ccttgccagg cacgcattgg caggtacaga tatgtgagcg gccccagcag cgctaggccg
     7681 gtaatcgcag gcaacttgcg cacgactatg cgccattggg gcatttggcg ggccacgaag
     7741 accaacagac ccagacccat gaagacgaag gcgcgctgat gggcaactcc ctggctgacg
     7801 aggaaggcca acaaatacag atcggctgtg cggccttccc gcccatagcg taacgccacc
     7861 agcacggtgg caaaggtcaa cgccatggtc atggtatgga cttcagccac agaggcaaat
     7921 gtccacatcg aagtcgtcaa cccgaacagc acggccgaac ttgctgcgat cacgggctga
     7981 acaccgaatg ccaggatcag actcgttaat agaccaatgc tgatagcgcc ccatgtcgca
     8041 gaatatagcg aagccccggc tgctggaggt agccccacga cgcgcagggc cgccacgaat
     8101 gctgagccga tcgctgtgta cagcgggtat ccggtgaaat gaatcgttcc ccaacgagga
     8161 agtgcattct gaatctcgcc gacatctgtg gcatagggat gagaactgcc attcacgtcc
     8221 agttgcagcg tggtcaagaa gagccccagg gctaggatgg ctgccccaat gaccgccatc
     8281 ctgggcgata gtttcagctc aaaacgcgac aagctgcgcg cgatactaga gaccatcaac
     8341 cccaccccct tcgccacgag agtttcgcac acgacgcgca ggcatcctga gccggccgcc
     8401 gtagaatcga acagccatcc atgccgcttc gccttacgcc aatccgaggc gagcatgcag
     8461 cggagaagcg cgccaggcgg ggatgcatga gttcatcccg gccggaatat gcagaagaac
     8521 cggacttgaa agggcaatca gcctgagcgc cagatggagc gtcgcgctca ctgacgtcgt
     8581 agctgatcga tgtggcaggt gcccgccctt atgtggcgga tgtcctcgta cgacagaggc
     8641 taggccgcga tcaactgctc gagcactccc tttgacttgg cctcgatctc ggcgtgcagg
     8701 ctcgcgccgt gcgcatccat cgtcacgacg gccgggaagt ccttgacctc gatcacccac
     8761 atcgcttccg gcac
//
LOCUS       DBNHXB010000422         4353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2979925, whole genome shotgun sequence.
ACCESSION   DBNHXB010000422 DBNHXB010000000
VERSION     DBNHXB010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2979925"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="AC162G_16690"
     CDS             complement(<1..276)
                     /locus_tag="AC162G_16690"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5077496.1"
                     /translation="MMTSGVVNGSDGVLIIDPGILPREIETIRWYVEQGEKPARFWVY
                     THHHWDHILGGQAFPSAHRLAHRCFPEAMSANRPLDEIRRFDGEYYVE"
     gene            complement(368..1270)
                     /locus_tag="AC162G_16695"
     CDS             complement(368..1270)
                     /locus_tag="AC162G_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR5077497.1"
                     /translation="MSKVMPLGDAIAQFVHDGDTVYAAGFTHLIPFAAGHEIIRQRKK
                     DLTLARATPDLIYDQMVAAGCARKVIFSYSGNPGVGSLRVMRKEMEAGRLEYEEYSHF
                     AMITRLTAGASGLPFLPMRPTGSDDLRRANPQYRTITCPYTGAELVTVPALNPDVAII
                     HAQRADRQGNAQFWGIIGEQKEAAFASKKVILTVEEIVDESVIRSDPNRTLLPDFIVS
                     AVCEVPFAAHPSYTQGYYDRDNPFYLEWDKISESQEATQAWLDEWVYGAQDRAGYWAK
                     LGVETHDRLKVKPMMSVPVNYGAF"
     gene            complement(1386..2339)
                     /locus_tag="AC162G_16700"
     CDS             complement(1386..2339)
                     /locus_tag="AC162G_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077498.1"
                     /translation="MSVKRLLLIGVAAAWAAGCARPIPVKVGAPVAFQHRTTVFTIDV
                     PDDWTRLQDQVPTEALGIFTDPTGQATIVAYVGLLDHRFGNEEGLRVVARLTANLLRR
                     PDDYQITGQEHRADGAFEVGFRYSRGEQNHAGLAVFRDTKLALSGVIISGPEAFWPRL
                     RMALLPYAESFEFDPEVVRGAYFDILEDVYYILVVPIDWPRQRSRFGTEVRSRFGRMS
                     IRLVQRDLGREIDRADLVDQTLATLHAGFNLVVKVLASEPQSDGRLKLTLRQADQQII
                     GYVETFDTHLVGIFFDVPDDRVEDYQSFMDFVYSMYISGLP"
     gene            complement(2414..3370)
                     /locus_tag="AC162G_16705"
     CDS             complement(2414..3370)
                     /locus_tag="AC162G_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077499.1"
                     /translation="MRKLLLWVGAALALCAACNNPGPIVVDDPIVAKHPTGVFTIRVP
                     RSWKLTQDTADTEALGIFTDPTGQVSLLTYVGLLERRLTDEEGFEIVNGLASTLLSQP
                     ADVQVTEQRRLQPDGAFEVMFTFTNQDGQKMKGRSIFHDTDLALSGVILTGPEPLWPG
                     LQSALAPFVDSFAVDSSIVHSIYFWPVEDSYYVLVAPIDWAQRRETDHVEVQSRNRQM
                     SIRVVQEELGVPVGATALADQAAASLRKNFGVNAKITSSESLPDGRLKVTLERGDRRH
                     IGYVEQFGTRFVGLFFQVPVDRVADYQPFMDFIYSTYVTGLP"
     gene            complement(3543..4280)
                     /locus_tag="AC162G_16710"
     CDS             complement(3543..4280)
                     /locus_tag="AC162G_16710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5077500.1"
                     /translation="MSGGPEGIIFQSAGQNLLGALYLGAGKGARPTAVVLHGIPGIEK
                     NTDIASALRDAGWNALVFHYRGSWGSEGDYTLPGIPDDVRAAIDELVSGKYAVDGERL
                     ALVGHSLGGWAAIVCAARDPRVKAAVTIGGISNMRTWYLSHEQATEYSRFLRGTSATE
                     LQWQIRALGATRNPVDVIGDIKSMPILIVHGNPDDAVSIDQATALHRASNGTAELVII
                     DGADHMFSRHRRQMVDTVVAWLIRNVK"
BASE COUNT          817 a         1426 c         1300 g          810 t
ORIGIN      
        1 ctcgacgtaa tactcgccgt cgaaccgccg aatctcgtcc agcggccggt tagcgctcat
       61 cgcttccggg aaacatcggt gcgccaatcg atgcgccgat ggaaacgcct gcccgccgag
      121 gatgtgatcc cagtgatggt gcgtgtacac ccaaaacctc gcaggcttct cgccctgctc
      181 gacgtaccaa cggatcgtct cgatctcgcg cggcagaata ccggggtcga tgatcagcac
      241 gccgtcgctg ccgttgacga cgccacttgt catcatgaac agtccactcg taaacacgcg
      301 aatctgcaag ctcaatgatc accccctaat ctccaatcac caatccctaa tgaccaatca
      361 tcaaacctca gaatgcccca taattgaccg gcacgctcat catcggcttg accttcaggc
      421 ggtcgtgcgt ctcgacgccc agcttggccc aatacccggc gcggtcttgg gcgccgtata
      481 cccattcgtc cagccaggct tgcgtcgctt cctggctctc gctgatcttg tcccattcca
      541 ggtagaacgg gttgtcgcga tcgtagtagc cctgagtata cgacgggtga gcggcgaacg
      601 gaacctcgca tacggcgctc acgatgaaat cgggaagcag cgtgcggttc ggatcagaac
      661 gaatcaccga ttcgtctacg atttcctcga ccgtcaggat caccttcttg ctggcgaacg
      721 cggcttcctt ctgctcgcca atgatgcccc agaattgtgc gtttccctgg cgatcggcgc
      781 gttgagcgtg aatgatcgcc acgtcgggat tcagtgcagg caccgtgacc agctccgcgc
      841 cggtgtaggg acaagtgatc gtgcgatact gagggttggc ccggcgcaga tcgtccgacc
      901 ccgtcggccg catgggcagg aaaggcaggc cggaggcgcc ggccgtgagc cgcgtgatca
      961 tcgcgaagtg cgaatactct tcgtactcca gccgcccggc ctccatttct ttgcgcatca
     1021 cgcgcagcga tcccacaccc gggttcccgc tgtaagagaa gatcactttg cgcgcgcagc
     1081 ctgccgcgac catctggtcg tagatcaagt ccggcgtggc gcgcgccagc gtcaggtcct
     1141 tcttgcgctg gcgaatgatc tcgtgacccg ccgcgaacgg gatcaggtgc gtgaatcccg
     1201 cggcgtagac cgtatcgccg tcgtggacga actgtgcaat cgcgtcgccg agcggcatca
     1261 ctttggacat gcttgactcc attcgtcggc gtccgcagcc gtgcagcacg cagacgcccg
     1321 gtcggatatg tcatgcaaag cctgctcaat ggcaagtgtc gggttgtaac cgatgacgct
     1381 ttgcatcagg gcaaaccgct gatgtacatt gaatatacga agtccataaa cgactggtag
     1441 tcctcaactc ggtcgtccgg cacgtcgaaa aagattccga ccaggtgcgt gtcgaacgtt
     1501 tcgacgtacc cgatgatctg ctggtcggct tgccgcagcg tgagcttgag ccggccgtcg
     1561 gactgcggct cgctcgccaa caccttgacg accaggttga atccggcatg gagcgtagcg
     1621 agcgtctggt cgacgagatc ggcccggtcg atctcgcgac ccaggtcgcg ctgcacgagc
     1681 cgaatggaca tgcgcccgaa gcgcgaccga acttcggtgc cgaacctgct tcgctgacgc
     1741 ggccaatcga tggggacgac caggatgtag tagacgtcct ccagaatgtc gaaatacgca
     1801 ccgcgcacca cctccgggtc gaactcgaac gattccgcat agggcagcag ggccatgcgc
     1861 agccgcggcc agaaagcctc cgggccactg atgatcacac ccgacaaagc cagcttggtg
     1921 tcgcggaaca cggccaggcc agcgtggttc tgctcgccgc ggctgtatcg gaagccgacc
     1981 tcgaacgcgc cgtcagcgcg gtgttcctgc ccggtaatct gataatcgtc cggccgccgc
     2041 agcagattgg cggtcagcct tgcaaccacg cgcaggcctt cttcgttgcc aaagcgatga
     2101 tcgagcaagc ccacgtatgc cacgatcgtc gcctggccgg tcgggtcggt gaagatcccc
     2161 agtgcctcgg tgggcacctg atcctggagc cgggtccagt cgtctggcac atcgattgtg
     2221 aacaccgtgg tgcgatgttg gaacgcgaca ggggcgccca ccttgaccgg gatgggtctg
     2281 gcgcagcccg ccgcccaggc agccgcgacg ccgatgagca acaggcgctt cacgctcatg
     2341 acaacactcc tgcttcgtcc cacgcttcga cacgccggaa accgatccgc ctcgcgcgtc
     2401 cctataagtc gtctcacggc aggccggtga cgtacgtcga gtagatgaag tccatgaacg
     2461 gctggtagtc cgccacgcga tcgaccggta cctggaagaa cagcccgacg aagcgcgtgc
     2521 caaactgctc gacgtagccg atgtgacgcc gatcgcctcg ttcgagcgtc actttcaggc
     2581 gaccgtccgg cagcgattca ctgctcgtga ttttggcgtt gacgccgaag ttcttcctca
     2641 acgaagcggc cgcctggtcg gccagcgccg tcgcgccaac aggcacgccc aactcctctt
     2701 gcacgaccct gattgacatc tggcgattgc gcgattgcac ttcgacgtgg tctgtctcgc
     2761 ggcgttgcgc ccaatcgatc ggcgcgacca ggacatagta gctgtcctcg acaggccaga
     2821 agtagatgct gtgcacaatg ctcgaatcca ctgcgaacga gtcgacgaac ggcgcgagcg
     2881 cgctttgcag gccgggccag agcggctccg ggccggtgag gatcacaccc gagagagcca
     2941 ggtccgtgtc gtggaagatc gaccgtcctt tcattttctg gccgtcttgg ttcgtaaacg
     3001 taaacatcac ttcgaacgcg ccgtcgggct gaaggcggcg ctgctcggtg acctgaacat
     3061 cggccggctg gcttaacagc gtcgacgcca gcccgtttac gatctcgaag ccttcctcgt
     3121 cggtcagccg tcgctccagc agacccacgt aagtcaaaag actgacctgt ccggtggggt
     3181 cggtgaagat gccgagcgct tcggtgtcgg ctgtgtcctg cgtcagtttc caactgcgcg
     3241 gcacgcggat cgtaaacacg ccggtgggat gtttcgctac gataggatca tccaccacga
     3301 tagggccggg gttgttgcag gcggcgcaga gcgccagcgc agcgccaacc cacagaagaa
     3361 gcttcctcat gcacatgccc tcatagagtg acgatcagcc atgccccagc cgccatccct
     3421 tcgccttcgc tcagggcagg ccttgcgccg agagcgagac gtgagtagtg cgcattgcga
     3481 cgaaatacgc aatacgcact acgcgcatcc tgtggatgat tcgattccgt cctggtgagc
     3541 gcctacttca cgttgcggat cagccaggcg accacggtgt ccaccatctg ccggcggtgg
     3601 cggctgaaca tgtggtccgc gccgtcgata atgaccagct ccgccgttcc gttcgaggcc
     3661 cggtgcagcg cagtggcctg atcgatcgac acggcgtcgt cgggattgcc gtggacgatc
     3721 aagatcggca tcgatttgat gtcgccgatg acgtcgacgg gattgcgcgt cgctcccaaa
     3781 gcgcggatct gccattgaag ctccgtcgcg ctggtgcccc tgaggaagcg cgaatactcg
     3841 gtcgcctgct cgtgagacag gtaccacgtc cgcatgttgc tgatgccgcc gatcgtcacg
     3901 gcagccttca cgcgcggatc gcgcgccgcg cacacgatgg ccgcccagcc gcccaggctg
     3961 tggccgacca gcgccagccg ttcgccgtcg accgcatact tgcccgaaac cagctcgtcg
     4021 atcgcggcgc gcacgtcgtc gggaatgccg ggcagcgtgt agtcgccttc gctgccccac
     4081 gagccgcgat agtgaaagac gagcgcattc cagccggcgt cgcgaagcgc cgaggcaatg
     4141 tcggtattct tttcgatgcc cggtatgccg tgcagcacga cggccgtcgg gcgcgcgcct
     4201 ttgcctgcgc ccaggtacag cgcgcccagc agattctgac ctgcgctctg aaagatgatg
     4261 ccttcgggac cgccggacat agtgggactc ctgcctcacc cctaccccgg cctccggcca
     4321 cccctccccc tctcctgaac caaaggttca gga
//
LOCUS       DBNHXB010000423         3539 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_967334, whole genome shotgun sequence.
ACCESSION   DBNHXB010000423 DBNHXB010000000
VERSION     DBNHXB010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_967334"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="AC162G_16715"
     CDS             complement(<1..1124)
                     /locus_tag="AC162G_16715"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014433.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HIR5077501.1"
                     /translation="MSTQFVFFSGKGGVGKTSMACTHAVRCADEGKRTLIVTTDPASN
                     LADVFEQAIGHQITPIEHVANLWAMEIDPDRATEEYIDRAMAPIRAAFPPQMVQVMEE
                     QMSGPCTAEVAAFDRFTDFLDVPSANGTTFDVVIFDTAPTGHTLRLLELPAEWSQSID
                     AASEGSGQTCIGPAAAIQDAKRKYERAMATMRDVSRATFIFVLHPEAIAIRETQRATA
                     ELGKLDIHTHQLIVNGIIPPDETVNPLFAARAEMQARYLRQIESELPLPARRMYLLPY
                     EIKGVDRLREVGRIFFDGVNEWATNQRVSESAIRRIRVNPRLSVSHVLPRVVPNSHSR
                     VLFFAGKGGVGKTVASCMTAVWLARRGYKTLLLTTDPAAH"
     gene            complement(1121..1480)
                     /gene="arsD"
                     /locus_tag="AC162G_16720"
     CDS             complement(1121..1480)
                     /gene="arsD"
                     /locus_tag="AC162G_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HIR5077502.1"
                     /translation="MTDAVTLIDVTPTHAGTAAVELFDPPMCCPTGLCGPTLDQTLLD
                     VNEMILALQAESVRVERYQMTSHPQAFLNNADVMRLVREKQMAALPITVVRGKVVKAG
                     AYPTLSEVTVHLNGATA"
     gene            complement(1522..1908)
                     /locus_tag="AC162G_16725"
     CDS             complement(1522..1908)
                     /locus_tag="AC162G_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5077503.1"
                     /translation="MDRIARPPTNLQTLADRLKVLAEPKRLLILHLLMEGVQCNCELG
                     DALDMPPNLISHHLRVLREAGLVDVERDSLDARWVYYSVNRAALDELNEVFGLFFDAE
                     RIKPRRPTCGPQGALVRLGDIAIARN"
     gene            complement(2009..2758)
                     /locus_tag="AC162G_16730"
     CDS             complement(2009..2758)
                     /locus_tag="AC162G_16730"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR5077504.1"
                     /translation="MRNPIQALAEAIHHKQPAVLATVIEVNGASPAKVGAQIALLPDS
                     STAGTAGGGKLEASILADARAALADGQPRLAHYTLTEEGTDAVGVLCGGEVRAFIQPY
                     MPPPQLVIVGGGHIGQPLKVMGEAAGFDVIIADVEPGLATVPGLDAVALTEDSYVVLI
                     TTDHVSDEAALRQVIASPARYVGMIGSRAKCQTILDHLHADSYTDEALARVYAPIGLD
                     LGGPTPQEIAVAILAEIIAVRRRGSGAMRSR"
     gene            complement(2760..>3539)
                     /locus_tag="AC162G_16735"
     CDS             complement(2760..>3539)
                     /locus_tag="AC162G_16735"
                     /inference="COORDINATES: protein motif:HMM:NF017964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 -
                     endo-alpha-N-acetylgalactosaminidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 100 family protein"
                     /protein_id="HIR5077505.1"
                     /translation="DNVLWYAALRAMAEMARALGLDGTRFDSMADDVRHKIRIVLWVG
                     PENEAEWGPSCPGHTEWKHTLSQVDPVLVKRPFFLPYVAFRDFGDYCDVFGNLLAILF
                     DVANPAQEKRILDYLHQVGIAEPYPVRVLHPVIHPGNKDWREYYRNNNLNLPEQYHNG
                     GIWPFVGGFYVAALVKAGRLNEARRQLEKLAELNRLGVEEEWEFNEWCHGRTGLPMGY
                     PHQAWSAGMYVFAHHCVTSGRVPILAGERSSLVEVNLPMGR"
BASE COUNT          659 a         1096 c         1099 g          685 t
ORIGIN      
        1 atatgtgctg ccgggtcagt cgtcagcagc agtgttttgt agccacgccg cgccagccac
       61 acagccgtca tgcacgaggc aaccgtcttc ccaacgccgc ctttgccggc gaagaacagt
      121 acgcggctgt ggctattcgg tacgacgcgc ggcaaaacat gggacacgga caaacgcgga
      181 ttgacacgga ttcgccggat cgctgattcg ctgactcgct gatttgttgc ccattcgttg
      241 acaccatcga agaatatcct gcccacctcg cgcagccgat ccacgccttt aatttcgtaa
      301 ggcaacaggt acatgcgacg cgccggtagc ggcaactcgc tctcgatctg tcgcaaatag
      361 cgcgcctgca tctcggcccg cgcggcgaag agcggattga ccgtctcgtc cggcggaatg
      421 atgccgttga cgatgagttg atgagtgtga atgtcgagtt tgcccagttc ggcagtggcg
      481 cgctgcgttt cccggatggc gatggcttca ggatgcagca cgaagatgaa agtggcccgc
      541 gagacgtcgc gcatcgttgc catggcgcgc tcgtacttgc gcttggcgtc ttgaatcgcc
      601 gccgccggcc cgatgcaagt ttgaccgctg ccttcgctgg cagcatcgat cgactggctc
      661 cattcggcgg gcagctcgag caggcgcagc gtgtgaccgg tcggcgcggt gtcgaagatg
      721 acgacatcga aagtggtgcc gttggcggat ggcacgtcga ggaaatcggt gaagcggtcg
      781 aaggcggcga cctcggcggt gcacggcccg ctcatctgct cttccatcac ctgcaccatc
      841 tgcggcggga aggcagcgcg gatcggggcc atggcccgat cgatgtattc ttccgtcgcg
      901 cggtcggggt caatctccat cgcccacaga ttcgccacgt gctcgatcgg ggtgatctga
      961 tgcccgatcg cttgttcaaa cacgtcggcc aggttcgagg ccggatcggt ggtcacgatc
     1021 agagtgcgct tgccttcgtc ggcgcagcgc acggcgtgcg tgcaggccat gctggtcttg
     1081 cccacgccgc ccttgccgga gaagaagacg aattgggtac tcacgcggtt gctccattca
     1141 agtgaactgt gacttcagac aatgtcggat aagcgccggc tttgacaacc ttgccgcgca
     1201 cgacagtgat tggcagcgct gccatctgct tctcgcgcac caggcgcatc acgtcggcgt
     1261 tgttgaggaa cgcctgcggg tggctggtca tctggtagcg ttccaccctc acgctttcgg
     1321 cttgaagcgc caggatcatc tcgttcacgt cgagcagtgt ttgatcgagc gtcgggccgc
     1381 acaggccggt cgggcagcac atcggcgggt cgaacaattc aacggcagcc gtgccagcgt
     1441 gagtcggagt cacatcgata agcgtcacag cgtcggtcat gttagttcaa tctcccttga
     1501 agtatcttgg caaaataaaa gtcagttcct tgcgatggcg atgtcaccca accgcacgag
     1561 cgcgccctgc gggccacacg tcgggcggcg cggcttgatc cgctccgcgt cgaagaacaa
     1621 gccaaacact tcgttgagtt cgtcgagtgc ggcccggttg accgagtagt acacccagcg
     1681 tgcgtcgagc gagtcgcgtt ccacatccac caggcccgcc tcgcgcaaga cgcgcagatg
     1741 atgcgaaatg agattgggcg gcatatccag cgcgtcgccc agttcgcagt tacactgcac
     1801 gccttccatt agcaggtgca ggatcagcaa gcgtttcggc tcggccagca ccttgagccg
     1861 gtcggcgagt gtttgcagat tggtcggagg gcgggcgatg cgatccatgt gtgaaagcaa
     1921 cggcagaata gttcaacgca tattgaagta tagtcgggga gagcagagga caacagagca
     1981 gaatgtcacg gggaaaggat gacgtttgtc agcgcgatcg catcgctcca ctgccgcgtc
     2041 gccgcacggc gatgatctcg gccaggatcg ccacggcgat ctcctgcggt gtcggcccgc
     2101 ccaggtccag accgataggg gcgtacaccc gcgccaatgc ttcgtcggta tagctatcgg
     2161 cgtgcaggtg atcgagtatc gtttggcact tggcgcggct gccgatcatt ccgacgtagc
     2221 gagcgggcga agcgatcacc tgccgcagcg cggcctcgtc cgatacgtga tcggtcgtga
     2281 ttagcacgac gtaggagtct tcggtgagtg ccacagcatc cagccccgga actgttgcca
     2341 ggccgggttc aacatcggcg atgatcacat caaagccggc agcttcgccc atcaccttca
     2401 gcggctggcc gatgtgccca ccgccgacga taacgagttg cggcggcggc atgtaaggct
     2461 ggatgaaggc ccgcacctcg ccgccgcaca gcaccccgac cgcgtccgtg ccttcctccg
     2521 tcaaggtgta gtgagccagg cgtggttgcc cgtcggccag agctgcccgc gcgtcggcca
     2581 gaatcgaggc ttcgagtttg ccgccgccgg ccgtgcccgc cgtcgagcta tcgggcagca
     2641 gggcgatctg cgccccgact ttcgcaggtg atgcaccgtt gacttcgatg acggtcgcca
     2701 atacagcggg ctgcttgtgg tggatggctt cggccagtgc ctgaatggga ttgcgcatat
     2761 catcgtccca tcggaagatt cacttccaca aggcttgagc gttcgccggc cagaatcggc
     2821 actctgccgc tcgtcacgca gtgatgggcg aacacataca ttcccgccga ccaggcttga
     2881 tgcggatagc ccatcggcaa gccggtacgt ccatggcacc actcgttgaa ctcccattcc
     2941 tcctcgacgc ctagccggtt gagttcggcc agtttctcca actgccgtcg agcctcgttc
     3001 agtcgcccgg ccttcaccaa tgccgcgacg taaaacccgc cgacgaaggg ccagattccg
     3061 ccattgtgat actgctccgg cagattcaag ttgttgttgc ggtagtattc gcgccagtcc
     3121 ttgttgcctg gatggatgac gggatgcagc acgcgcaccg gatacggctc ggcgatgccg
     3181 acctgatgca gatagtcgag gatgcgtttc tcttgcgcgg gatttgccac atcgaagagg
     3241 atcgccagca ggtttccgaa cacgtcgcag tagtcgccga agtcgcggaa ggcgacgtag
     3301 ggcaggaaga acggccgctt gaccagcacc ggatcgacct ggctgagcgt gtgcttccac
     3361 tcggtgtggc cggggcaaga cggcccccac tcggcttcgt tctccggccc gacccacagc
     3421 acgatccgaa tcttgtgccg cacatcatcg gccatagagt cgaaccgcgt cccatcgaga
     3481 cctagcgcac gcgccatctc ggccatcgct cgcagcgccg cgtaccacag cacgttgtc
//
LOCUS       DBNHXB010000424         5065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2142380, whole genome shotgun sequence.
ACCESSION   DBNHXB010000424 DBNHXB010000000
VERSION     DBNHXB010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2142380"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="AC162G_16740"
     CDS             <1..1213
                     /locus_tag="AC162G_16740"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR5077506.1"
                     /translation="LSHLAQSIATSPTMAMNEQARLLRERGEAVIHLGIGEPKNKAPI
                     NAILSGAARLGSGDVKYSPADGIPSLKKAIIRYTEDNYGVTVAPENVIVTSGAKQSVF
                     NILYTILNPQDEVIVLAPYWVSYPEIVKMVLGAPVIVTPEDGSFTPRMADIERAIGRN
                     TKAIIVNSPNNPSGAVYPEELIAAIVETCERKGIYLIMDDIYHKLVFDGKHAPPCYQF
                     TDKDVEDSRLIVVNGVAKLYGMTGFRVGWTIAPRRLVEIMTNVQSQTTSCVSVLLQAA
                     AEGALTGMQSLVESLRLTIQNNRDVIIQELKAFDGVRVTPPEGTFYCLPDFRAYDGDS
                     VALAKFLLERALVVTVPGKEFGMEGHLRLSYAGTVKDVTDGIERIRWALDPNSPNEIY
                     IGDRRMVRDWI"
     gene            1276..2907
                     /gene="pckA"
                     /locus_tag="AC162G_16745"
     CDS             1276..2907
                     /gene="pckA"
                     /locus_tag="AC162G_16745"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR5077507.1"
                     /translation="MNNILNIKTPAEAQAGAYKCDYGLDAIGLANMRQVYWNLPVEAL
                     YEESVFRGEAKITHQGPIVANTGKHTARSASDKFVVREPASEAHVWWGQYNRPFSLDK
                     FDDLFGRMQGFLQGRDVFVQDCYAGADIHYRMPIRIVTELAWHSLFARNMFLAPRTHE
                     DYRHHVPEFTVICAPSFKGIPQVDGTASNTFIVLNFEKRLCIIGNTAYAGEIKKSIFT
                     ILNYLLPLQGVMPMHCSANVGRSGDAALFFGLSGTGKTTLSADPKRGLIGDDEHGWSD
                     EGVFNFEGGCYAKVIALSPTAEPQIFATTRRFGTILENVVYDPVTRLIDLDDDSTTEN
                     TRASYPLEFIDNAVAGKCGGHPKNIILLTCDASGVMPPIARLTTEQAMYQFISGYTAK
                     VGGTEIGLREEPEITFSACFGAPFMVHPPSFYADLLQRKIERHGVNCWLVNTGWVGGP
                     YGIGKRISIAHTRALLDAALSGALLGVEYRADPVFGFQIPARCDGVPESVLDPSSSWP
                     GNDVYMQRYRELASRFVQNFRKFDAPHLVAAGPRV"
     gene            2986..3558
                     /locus_tag="AC162G_16750"
     CDS             2986..3558
                     /locus_tag="AC162G_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015163759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HIR5077508.1"
                     /translation="MPTRKTALTGPIYQLKITLKNSRPPIWRRIQVTDDTTLAKLHRI
                     LQIAMGWSDYHLHDFTIYGARYSEPSPDDWEPVKDERRYKLKQLVPGEKSKFVYQYDF
                     GDSWDHEILVEKTGLPREPGVKYPRCLAGKRACPPEDVGGVWGYREFLEAIRDPEHPE
                     HDGYLEWAGGEFDPEAFDLEEVNRALRSMR"
     gene            3674..3913
                     /locus_tag="AC162G_16755"
     CDS             3674..3913
                     /locus_tag="AC162G_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077509.1"
                     /translation="MIAHRRYITIKDPKQVVLSDLPFQPGQRVEVVLIAEEDRPGSRL
                     HELREVFRATQALPQAQVLSEDEIAAEIAAYRMGR"
     gene            3910..4320
                     /locus_tag="AC162G_16760"
     CDS             3910..4320
                     /locus_tag="AC162G_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017653674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HIR5077510.1"
                     /translation="MKVLIDTNVLVSAALKDKDPEAVILFVVSRADYEWIVSPEILRE
                     YKEVLSRDKFGLPAEVRQKWFDLLDALTTVVEVDVHVDFPRDQKDAKFLACALAADVE
                     FFVTGDRDFAQAYKLVNTTILSVSLFKKFVMQVE"
     gene            complement(4425..4865)
                     /locus_tag="AC162G_16765"
     CDS             complement(4425..4865)
                     /locus_tag="AC162G_16765"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0043137 - DNA replication, removal of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HIR5077511.1"
                     /translation="MGGPTKKQGTPPHITIYTDGASSGNPGPGGWAAILRHDETGRVK
                     ELSGRRDRTTNNEMEMWAVEQALGAIKVPGSIVTIHTDSENVIGWLAEGWKCRFPHIR
                     RIRDNIQALCSEMRLDVSYVKVAGHAGDRLNERANALAQAAIGK"
     gene            complement(4896..>5065)
                     /locus_tag="AC162G_16770"
     CDS             complement(4896..>5065)
                     /locus_tag="AC162G_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR5077512.1"
                     /translation="RDRDEGQGMTAVVGRVRPCPLFEIKFVVLSHNTIRGAAGGSILN
                     AELLLAQGYLN"
BASE COUNT          985 a         1565 c         1595 g          920 t
ORIGIN      
        1 cctcagtcat ctggcgcagt caatcgccac gtcgccgacg atggcgatga acgagcaggc
       61 acgcctcctg cgcgagcgcg gcgaggcggt catccacctg ggcatcggcg agcctaagaa
      121 caaggcgccg atcaacgcca tcctgagcgg cgcggcgcgc ctgggcagcg gcgacgtcaa
      181 gtactcgcct gccgacggta tcccctcgct caagaaggcg atcatccgtt acaccgaaga
      241 caactacggc gtgaccgtcg cgccggagaa cgtcatcgtt acgtcgggcg cgaagcagtc
      301 ggtcttcaac atcctgtaca cgatcctcaa cccgcaggac gaggtgatcg tcctcgcgcc
      361 ctactgggtc agctacccag agatcgtcaa gatggtgctc ggcgcgccgg tgatcgtcac
      421 gccggaggac ggcagcttca ccccacgcat ggcggacatc gagcgcgcga tcgggcgtaa
      481 cacgaaggcg atcatcgtca acagcccgaa caatccatcg ggcgccgtgt acccggagga
      541 gcttatcgcg gcgatcgtcg agacctgcga gcgcaagggc atctacctca tcatggacga
      601 catctaccac aagctcgtct tcgacgggaa gcatgcgccg ccgtgctacc agttcaccga
      661 caaagacgtc gaggactcgc ggctgatcgt cgtcaatggc gtggccaagc tgtatgggat
      721 gaccggcttc cgcgtcggct ggacgatcgc gccccggcgg ctcgtcgaga tcatgacgaa
      781 cgtgcagtcg cagacgacgt cgtgcgtatc ggtgctgctg caggcggcgg cggaaggcgc
      841 gctgaccggc atgcagagcc tggtcgagag cctgcgcctg acgatccaga acaaccgcga
      901 cgtgatcatc caggaactga aagcattcga cggcgtgcgt gtcactccgc ccgaggggac
      961 gttctactgc ctgcccgact tccgcgccta cgacggcgat tcagtcgcgc tggcgaagtt
     1021 tctattggag agagcgctcg tcgtcaccgt gccaggcaag gaattcggaa tggaaggtca
     1081 cctgcggctg agctacgcgg gcacagtgaa ggacgtgacg gacggcattg agcgcatccg
     1141 ctgggcgctc gatccgaatt cgcccaacga gatctacatc ggcgaccggc gcatggtgcg
     1201 cgactggatt taggagactt cggaagtctc caaaagactt ccgaagtctg aaattcaagc
     1261 taagggtatc gacctatgaa caacattctg aacatcaaga cgcctgccga agctcaggcc
     1321 ggggcttaca agtgcgacta cgggctggac gcgatcggcc tggcaaacat gcggcaggta
     1381 tattggaatc tgccggtcga ggcgctgtat gaagagagcg tctttcgcgg cgaggcgaag
     1441 atcacacatc aggggccgat cgtcgcgaac accggcaagc ataccgcgcg ctcggcgagc
     1501 gacaagttcg tcgtgcgcga gccggcgagc gaggcgcacg tgtggtgggg ccagtacaac
     1561 cggccgttca gcctcgacaa gtttgacgac ctgttcgggc ggatgcaagg cttcctgcag
     1621 gggcgcgacg tgttcgtgca agattgttac gccggcgccg atatacacta tcgcatgccg
     1681 atccgcatcg tcaccgagct ggcgtggcac agcctgttcg cgcgcaacat gtttctcgcg
     1741 ccccgcacgc acgaggacta tcgccatcac gtgcccgagt tcaccgtgat ctgcgcgccg
     1801 tcgttcaagg gcatcccgca ggtcgacggg acggcgtcga acacgtttat cgtcctcaac
     1861 ttcgagaagc ggctatgcat catcggcaac accgcctacg ccggcgagat caagaagtcg
     1921 atcttcacca tcctgaacta cctgctgcct ctgcaaggcg tgatgccgat gcactgctcg
     1981 gcgaacgtgg gccgaagcgg cgacgcggcg ttgttcttcg ggctatccgg cacgggcaag
     2041 acgacgctct cggccgatcc caagcgcggg ctgatcggcg acgacgagca cggctggagc
     2101 gacgagggcg tgttcaactt cgagggcggc tgctacgcga aagtgatcgc gctgtcgccg
     2161 acggccgagc cgcagatctt cgcgacgacg cggcgcttcg ggacgattct cgaaaatgtc
     2221 gtgtacgacc cggtcacgcg gctgatcgac ctcgacgacg actcgaccac cgagaacacg
     2281 cgcgcgtcgt acccgctcga attcatcgac aacgcggtag ccgggaagtg cggcggccat
     2341 ccgaagaaca tcatcctgct cacgtgcgac gcgtcgggcg tgatgccgcc catcgcgcgc
     2401 ctgacgaccg agcaggcgat gtaccagttc atctccggct acacggcgaa ggtcggcggg
     2461 acggagatcg gcctgcgcga ggagccggag atcaccttca gcgcgtgctt cggcgcgccg
     2521 ttcatggtgc acccgccgtc gttctacgca gacctgctcc agcgcaagat cgagcgccac
     2581 ggcgtgaact gctggctggt gaacacaggc tgggtcggcg ggccatacgg catcggtaag
     2641 cgcatcagca tcgcccacac gcgcgccctg ctcgacgcgg cgctgagcgg cgcgctgctt
     2701 ggcgtcgaat accgggccga cccggtgttc ggtttccaga tcccggcacg ctgcgacggc
     2761 gtgccggaga gcgtgctcga tccgtcgtcg tcgtggccgg gtaacgacgt gtacatgcag
     2821 cgctaccgcg agctggcgtc gcgcttcgtc cagaacttcc gcaagttcga cgcgccgcac
     2881 ctggtcgcgg cggggccgcg ggtgtagttt tgggacgcgg atgaacacgg atgaacgcgg
     2941 ataggattga ttcctgcgga atctctatgg aaaggagcag cgtcgatgcc gacccggaaa
     3001 acggccctga ccggcccgat ctatcagctc aagatcacgc ttaaaaactc tcgaccgccc
     3061 atctggcggc gcattcaggt gacggacgat acgacgcttg ccaagctgca ccggatcctg
     3121 cagatcgcga tgggttggtc cgattaccat ctgcacgatt tcacgatcta cggcgcccgc
     3181 tacagcgagc caagccctga cgactgggaa ccggtgaagg acgagcggcg ctacaagttg
     3241 aagcagctcg tccctggtga aaagtccaag ttcgtctatc agtacgactt cggcgacagt
     3301 tgggaccacg aaatcctcgt ggaaaagacc gggctgccgc gggagccagg cgtgaagtac
     3361 ccgcgctgcc tggccgggaa gcgcgcctgc ccgcccgagg atgtcggcgg agtgtggggc
     3421 taccgtgaat tcctcgaggc cattcgcgac cccgaacatc ctgagcacga tggctatctg
     3481 gagtgggccg gcggcgagtt cgacccggaa gcctttgacc tggaggaggt taaccgggca
     3541 ttgcgctcaa tgagataacc gcgcagggct tggtccccct accctatcag ttgggggttg
     3601 cgcaccggat gtcgcaatct cttcgcgaat tcgcagtata cttacaggtg gacgatctac
     3661 caggaggcgt ctaatgatag cacacagaag gtatatcacg atcaaggatc ccaaacaggt
     3721 cgtgctgtct gacttgccct tccagcccgg ccagcgcgtc gaggttgtgc tgattgcaga
     3781 ggaggaccgg cccggatcgc gtcttcacga gttacgggaa gtgttcaggg cgacgcaggc
     3841 actcccgcag gcccaggttc tgagcgagga tgagatcgcg gctgaaatcg ccgcctatcg
     3901 gatgggacga tgaaagtcct gatcgacacc aatgtgctgg tgtcggcggc tctgaaggat
     3961 aaggatccgg aagcagtcat cctgtttgtc gtttctcgcg ccgactacga atggatcgtc
     4021 tcgcctgaaa ttctgcgcga gtacaaagaa gtgctcagcc gagacaagtt cggcctgccc
     4081 gctgaagtac gacagaagtg gttcgacctc ctcgacgcgc ttacaacagt tgtcgaagta
     4141 gatgtccacg ttgattttcc ccgtgatcag aaggacgcca agtttctggc gtgcgccctg
     4201 gccgccgacg tcgagttctt tgtcaccggc gatcgagact ttgcgcaggc ttacaaactg
     4261 gtcaacacga caattctgtc tgtgtcgcta ttcaagaaat tcgtgatgca ggtagagtag
     4321 gacgccgcac ctcgtggacg agtcaaatcc accgatccgt gtagacctgg caggtcttcg
     4381 cgatccaacc catggctgac accggcagat aagacctgcc aggtctattt tccgatagct
     4441 gcctgcgcca gtgcgttggc tcgttcgttc agtcgatcgc cggcgtggcc ggcgactttg
     4501 acgtaggaca cgtccaggcg catctcgctg cagagggcct ggatgttgtc cctgatgcgc
     4561 cgaatgtggg gaaacctgca cttccagccc tcggccagcc agccgatcac gttctcgctg
     4621 tctgtgtgga tggtgacgat cgagccgggc actttgatcg cgcccagcgc ctgctcgacc
     4681 gcccacatct ccatctcgtt gttcgtcgtc cggtcacggc ggccgctcag ctctttgacg
     4741 cggccggttt cgtcgtggcg caggatggct gcccagccgc ccgggccggg attgccggag
     4801 cttgcgccgt cggtgtagat cgtgatgtga ggcggcgtgc cttgtttctt cgtaggtccc
     4861 cccaccctac tgacctccgc agttgagcct cccggtcaat tcaggtagcc ttgggcgagc
     4921 agcagctcgg cattcaaaat cgagccgccg gctgcgccac ggattgtgtt gtgactgagg
     4981 acgacgaatt tgatctcgaa cagcgggcag ggccgcacgc gaccgaccac cgccgtcatc
     5041 ccctgccctt catctcgatc gcgtc
//
LOCUS       DBNHXB010000425         3667 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3987429, whole genome shotgun sequence.
ACCESSION   DBNHXB010000425 DBNHXB010000000
VERSION     DBNHXB010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3987429"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="AC162G_16775"
     CDS             complement(<1..372)
                     /locus_tag="AC162G_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077513.1"
                     /translation="MSISQRIWNALKNVTIVMSLAVNIVLIVVVLVLISQIGSIKATL
                     NGVLTQLDTAFSSLGAAVIQDTIHIDQRVPVRFDLPVDQNVTVVTTAPVPLTIPASFS
                     LGPFGHINGTVSLALPQGLSLP"
     gene            complement(717..>3667)
                     /locus_tag="AC162G_16780"
     CDS             complement(717..>3667)
                     /locus_tag="AC162G_16780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR5077514.1"
                     /translation="PRFVLAAEQAGIKPIIGVELTLANGGGHLTLLAENSAGYANLCR
                     LITLARRDQQKGIAALAWRLLADYAKGIIALSGCRRSEIARALLNHDFDRAAHVAERF
                     AAIFGKDNFFLEMQRHRERGDRRLSAGLAALSQRLGLRLVATGNVHYLEPSQARLHDV
                     LTCIRHRAPLDSADAILRTNTEYTFRSPAEIDALFAEWQDALQSTHDIAERCKVCWPE
                     GPQVFPTLPTPNDQPALAFLRQLCEDGLARKTPPPAQSHYRAQLDREIAVIAEQNLID
                     YFLVVWDLVRFARERGILCQGRGSAANALVSYLLDISPVDPLSAGLVFERFLSRERVS
                     APDIDIDFDAARREEVIQYIYRRYGHDHAAMACTTVTFGARQAVRDVGMALGFSEETL
                     DRISERIDTFGNAADLPASPSLRAAFGAQLDSPRWQQLIALASAIDGYPRHLGIHNGG
                     MVISGPPLVEQIPVEPASMEDRTVVQWDKDALEAAGWVKLDVLGLRTLSAIADACAMI
                     ETRTGQRPNPSASSGQALAALRFNDPKVFALIRRGETVGVFQVESRAQASLIPRFKTR
                     SFADIVIQIALIRPGPVQANMVHPYLRRRDRFEPIRYLHPLLRPALQETLGVILFQEQ
                     VLKVAHDFAGFTAGEGELLRRVLGHKRADIQIEMFRDKFLAGARAKGVSLKVAEQVFE
                     QLKAFGGYSFSKAHAAAFAVITYWSAWLRCYHPLAFFAGLLRHQPMGFYPKHVVVSDA
                     QRAGVKFLPVDLRISQSETTVEGDAIRLGLSDLHGFGPEQIELLEAERQRGLFKSLAD
                     LVKRTNLDRRHVETLVLAGALDYLGERRQLLWDMAEAYRLARRPREMPLHSPDERPRL
                     KPMDKPTRLSTAFAATGVSLEGHLTSLRRDAFTRAGARSIKDLPYLKNGQRVRIGGVV
                     VAHQHPPTAKGYGFLAVEDPDGMVNVVIAPDVYAQYRDAIHGLFVIVDGVLQRDHGAI
                     NVVAQEVSKV"
BASE COUNT          767 a         1048 c         1156 g          696 t
ORIGIN      
        1 gggaagtgac aaaccctgcg gcagcgcaag agagactgtg ccgttgatgt gcccaaaagg
       61 ccccagcgag aagctggccg ggatggtcag cggcacgggg gcggtggtca cgacggtcac
      121 attctggtcg accggcagat cgaagcgcac cggcacacgc tggtcgatgt ggatagtgtc
      181 ctggatgacg gccgcgccca gcgaggaaaa ggccgtgtcg agctgcgtca gcacgccgtt
      241 cagcgtcgcc ttgatgctgc cgatctggct gatcagaacc aggacgacga cgatcaggac
      301 gatgttgacg gcgagcgaca tgacgatcgt gacgttcttc agggcgttcc agatgcgctg
      361 cgaaatggac acggcaacct cctcagaact cgattccgga gtgcaggcgc gaatcttatc
      421 attaccgccc tgtgagtgca aacggcatat caggatgtgg cgatcatcat ctcgccccgg
      481 caagccgccg gtgccgcagt gttgcgctca gtgcgtcccg ttgtaaaacg ttggcgtgac
      541 tggccgatgg agaagtacgg ctggtgctga caaaatgaca ggctacgtcg gctattgacc
      601 atctggctgg ctgctgggtc gcgagtatct gcctcaatgc ggcactgagg agtgtgtctg
      661 ttagttatgt aaatgtttga cgtgaaacaa tggccgtgca caataaagga acatactcag
      721 accttagaca cttcctgcgc cacgacgttt atagccccgt ggtccctttg cagtacccca
      781 tcgactataa cgaagaggcc gtggatagcg tctcgatatt gagcatagac atcgggcgcg
      841 atcaccacgt tgaccatgcc atcggggtct tctacagcga gaaagccata acccttggcc
      901 gtaggcggat gctggtgcgc cacgaccacg ccgccaatcc tcacacgctg gccgttcttg
      961 agataaggca agtctttgat cgagcgtgcc ccggcccgcg tgaaggcatc tcgccgtagt
     1021 gaagtcaaat gcccttcaag gctaacacct gtcgccgcga aagcggtcga taagcgtgtc
     1081 ggcttgtcca ttggcttgag gcgcgggcgt tcgtcagggc tatgcaaagg catttcgcgg
     1141 ggccgccgtg cgaggcgata ggcttcggcc atgtcccata acagttgccg ccgctcgccg
     1201 aggtaatcga gcgcgcccgc caagaccagc gtttcgacat ggcggcgatc caggttggtc
     1261 cgctttacca gatcggcaag cgacttgaat agcccgcgct gccgttcggc ctctaacagt
     1321 tcaatttgct cagggccgaa gccatgcaga tcgcttagtc caagacggat cgcatcaccc
     1381 tctacagttg tctcactctg cgagatgcgt aagtcaaccg gcaggaactt cacccccgcg
     1441 cgctgggcat cgctgacgac gacgtgcttg gggtaaaagc ccatcggttg gtgtcgcagt
     1501 agaccggcga aaaacgccag tggatgatag cagcgcagcc aggcggacca ataggtaatg
     1561 acggcaaaag ctgccgcgtg cgcctttgag aaactgtagc cgccaaaggc tttgagctgc
     1621 tcaaaaacct gctcggccac tttgagcgat acgcctttgg cccgcgcccc cgcaaggaac
     1681 ttgtctcgga acatctctat ctgtatgtcc gcccgtttgt gtcccaacac gcgccggagt
     1741 aactcgccct ctcccgccgt gaacccggcg aagtcgtggg cgactttcaa cacttgctct
     1801 tgaaagagaa tcacgccgag cgtttcttga agtgcgggcc ttagtagcgg gtgcaggtag
     1861 cggatcggct cgaaccgatc gcgccggcgc aagtaaggat gaaccatatt ggcctgcacc
     1921 gggccgggac ggatcagcgc gatctgaatg acgatgtcag cgaaggatcg cgtcttgaag
     1981 cgtgggataa gcgatgcctg cgcgcggctt tcgacttgaa aaacgccgac cgtctcgccc
     2041 cgccgaatca gggcgaacac tttgggatcg ttgaagcgca gcgcggccag agcctgccct
     2101 gagcttgccg aagggttggg acgctggccg gtgcgcgttt cgatcatcgc gcaggcatcg
     2161 gcgatggccg agagtgtgcg tagtcctagc acatctagtt tgacccagcc cgccgcctcc
     2221 aatgcgtcct tatcccattg aacaacggtg cgatcctcca tcgaagccgg ctctaccggg
     2281 atttgctcga cgagcggcgg gccagagatg accatgccac cattgtgaat gccgagatga
     2341 cgcggatagc cgtcgatagc agaagcaagc gcgatcagtt gctgccagcg cggtgagtcg
     2401 agttgcgcgc cgaaggctgc gcgaaggctg ggtgaagccg gcaggtcggc ggcgttgccg
     2461 aaggtgtcga tacgctcgct gatccgatca agtgtttctt ccgaaaagcc caacgccatg
     2521 ccgacatcgc ggacggcctg acgcgcgcca aaggtcacag tcgtacaggc cattgccgcg
     2581 tggtcgtgtc cgtagcggcg gtaaatgtac tggatcactt cttcgcggcg ggccgcatca
     2641 aagtcgatgt cgatgtccgg cgcgctgacg cgctcgcgtg acagaaaccg ctcgaagacc
     2701 agaccggcgg aaagcggatc gacggggcta atatccagca gataagagac gagagcgttt
     2761 gcggcacttc cacgaccctg acacaaaatg ccccgctcgc gcgcaaagcg gaccaaatcc
     2821 catacgacaa gaaagtagtc gataaggttc tgctccgcga tgacggctat ctcgcggtcg
     2881 agctgcgcgc ggtaatggga ctgtgcgggc ggcggggttt tgcgcgcgag accgtcctcg
     2941 cagagttggc gcaggaaagc gagcgccggt tgatcgttcg gcgtgggtag tgtcggaaag
     3001 acctgcgggc cttcgggcca gcaaacttta catcgttcgg cgatgtcgtg cgtagattgg
     3061 agcgcatcct gccattcggc gaatagcgcg tctatctccg cgggcgagcg gaaggtgtat
     3121 tcggtgttcg ttcgcagaat cgcatcggca ctgtcgagcg gggcgcgatg acggatgcaa
     3181 gtgagtacat catgcaggcg cgcttgtgag ggctcaagat aatggacgtt gcctgtagcg
     3241 accagacgca agccgagacg ctgcgataac gcggcaagtc cggcgctgag gcgccgatcg
     3301 ccgcgctcac gatgacgctg catctcgaga aaaaagttgt ctttcccgaa aatggccgcg
     3361 aagcgttccg caacatgtgc ggcccggtcg aagtcgtgat tgagcagcgc gcgggcaatt
     3421 tcgctgcggc ggcaacccga cagggcaatg atgcctttag cataatcggc aagaagtcgc
     3481 cacgccagcg cggcaatgcc cttttgctgg tcgcgccggg cgagggtgat gaggcgacag
     3541 agattggcgt agccggcgct gttttcagct aacagtgtga gatgaccgcc cccgttggct
     3601 aacgtcagtt caacaccgat gatcggcttg atgccggctt gctctgcggc taacacaaaa
     3661 cgcggta
//
LOCUS       DBNHXB010000426         3433 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_464479, whole genome shotgun sequence.
ACCESSION   DBNHXB010000426 DBNHXB010000000
VERSION     DBNHXB010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_464479"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="AC162G_16785"
     CDS             complement(<1..740)
                     /locus_tag="AC162G_16785"
                     /inference="COORDINATES: protein motif:HMM:NF039189.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HIR5077515.1"
                     /translation="MTTIRRLYLYLASFIGMGVALAGASILIRLMVDWGFDAFRGFFI
                     ASSAPALALLIAGGATWSFYWRVAQREAIFAPEERASGTRKLYLYGTLTLSLLGALIL
                     LQQALADLLVRLLDQGFSSVDKPWTPFLIAAVLGAAWRWHKQIADAERTAGADGMRGG
                     DLRRAYWFASALYGAAAAAGGLMVFISGVVWRLEDRTPQPFGFFGNAPWIHILVPPLM
                     QVLVAGLAIWMFWLPSQKAAASGDEAERS"
     gene            complement(813..2549)
                     /gene="aceK"
                     /locus_tag="AC162G_16790"
     CDS             complement(813..2549)
                     /gene="aceK"
                     /locus_tag="AC162G_16790"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HIR5077516.1"
                     /translation="MSSERDDTVLVRYAVDAISRAFEAYRQQFAEITRRATARFENRD
                     WHGMQRDAVERLDVYARVIALLVVDIRSLLAERVHDRALWAQMKTTYSAWIARRADFE
                     LAETFFNSVTRRIFATIGVDPKIEYVDSDFDAPPSVSQEPVYITFAGQGSTHTLIAEI
                     LAHYRFTIPYQDLDRDARLVAAEIESQWRLACGAQPIESVEVMKPVFYRNQGAYLVGR
                     VRGGDRFVPLVIALHNATEGVLVDAVLLTEDEVSIVFSFTRSYFHVDVEQPHDVIRFL
                     RSIMPLKRVAELYIALGYNKHGKTELYRDLLRHLHQSDDKFVIAPGDRGMVMVVFTLP
                     SYDIVFKVIRDKCLPPKTATRQDVMGRYQLVFKHDRAGRLVDAQEFEHLAFTVERFSD
                     ELLAELREAAPDSVTIQDGAVDIKHLYTERRITPLNLYLRDAEPVAIAEAVLDYGEAI
                     KDLAATNIFPGDLLLKNFGVTRHGRVIFYDYDELCLVTDCNFRDMPRARDDEEEMQSE
                     PWFYVGPNDIFPEEFIRFLGFPGHARETFLEAHRDLLTAGYWRRVRERHIDGEIIDIF
                     PYRQSKRLRARS"
     gene            complement(2646..>3433)
                     /locus_tag="AC162G_16795"
     CDS             complement(2646..>3433)
                     /locus_tag="AC162G_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR5077517.1"
                     /translation="FLALFKEAFPKEFGKIRFPETSGIGIKPVSRDGSERLIRAAINY
                     AIANKRKSVTFVHKGNIMKFTEGAFRNWGYALAEREFGDRVYTWSQWERTRADAQRGE
                     EAANAEQKAELARGKVLVKDAIADITLQQVLTRPDEFDVIATMNLNGDYLSDALAAQV
                     GGIGIAPGGNINYVTGHAVFEATHGTAPKYANLDKVNPGSVILSGEMMLRYMGWAEAA
                     DRVIKGMDGAIGSKTVTYDFARLMEGAKEVKCSEFGDAVIAHM"
BASE COUNT          669 a         1093 c         1021 g          650 t
ORIGIN      
        1 gagcgctcgg cttcatctcc tgaagcggcc gccttctgcg agggcagcca gaacatccag
       61 atcgcgagac ccgcgacaag gacctgcatc aacggcggca ccaggatatg gatccacggc
      121 gcgtttccaa agaacccgaa cggctgcggc gtccggtcct ccagacgcca caccactcca
      181 gagataaata ccatcaggcc gcccgccgca gcggcggcgc cgtagagggc cgaggcgaac
      241 cagtaagcgc gcctgaggtc gccgccgcgc atgccgtctg ctccagccgt tcgctcggcg
      301 tcggcgatct gcttatgcca gcgccaggcc gcgcccaaca cggcggcaat caagaacggc
      361 gtccatggct tgtcgacgct gctaaagccc tgatcgagca gtcgaacgag caggtcggcc
      421 agtgcttgct gcagcaggat caacgcgccg agcaagctga gcgtcaaggt gccatacaga
      481 tagagcttgc gcgttcccga cgcccgctct tccggggcga aaatggcttc gcgctgggcc
      541 acccgccagt agaaactcca ggtagcgccg ccagcgatga gcagcgcaag cgccggcgcg
      601 ctggaggcga tgaaaaaccc tctgaatgcg tcgaatcccc agtcgaccat gagcctgatc
      661 aggatggagg cgccggccag cgccacaccc atcccgataa acgaagcgag gtaaaggtac
      721 aagcgccgga tcgtcgtcat gtcatctctc cacaggcctc agcgtcacgc tgtctttgag
      781 acgacgcgga atcgacgcgc gttccctggc ggtcaggatc gtgcacgcaa acgcttgctt
      841 tgccggtagg ggaaaatgtc gatgatttcg ccgtcgatgt gccgctcccg cacccgtctc
      901 cagtagcctg ccgtgagcaa gtcgcgatgc gcttctagga aggtctcgcg cgcatgcccg
      961 ggaaagccca gaaagcggat gaattcttcg gggaagatat cgttagggcc gacgtagaac
     1021 cacggctcag actgcatctc ctcctcgtcg tctctggcgc ggggcatatc cctgaagttg
     1081 cagtccgtta ccagacataa ctcgtcgtag tcgtagaaga tgacccgtcc gtggcgcgtc
     1141 acgccgaagt tcttgagcag caggtcgccc ggaaagatgt tggttgcagc caggtctttg
     1201 atggcttcgc cgtagtccag cacagcctcg gcgatagcca ccggctccgc atcgcgcagg
     1261 tacaggttga gcggcgtgat gcgccgttcg gtgtacaggt gcttgatatc cacggcgcca
     1321 tcctggatgg tcacgctgtc gggcgccgcc tccctcaact ccgccagcag ctcgtccgaa
     1381 aagcgctcga cagtgaatgc caggtgctcg aactcctgcg cgtcgaccag gcggcccgcg
     1441 cggtcgtgct tgaacaccag ttgataccgg cccatcacat cctgccgggt cgccgtcttc
     1501 ggcggcaggc atttgtcgcg tatcaccttg aagacgatgt cgtacgaggg tagcgtgaac
     1561 acgaccatga ccatgccccg atcacccgga gcgatcacga acttgtcgtc ggactggtgg
     1621 agatgtcgca gcaaatcgcg ataaagctcg gttttgccgt gcttgttgta gccgagcgcg
     1681 atgtacagct cggcgacgcg cttcaagggc atgatcgatc tcagaaagcg gatcacgtcg
     1741 tgcggctgct cgacgtcgac gtgaaaatag gagcgcgtga agctgaagac gatgctcacc
     1801 tcatcttccg tcaagagcac agcatctacc aggactccct ctgttgcatt gtgcagcgcg
     1861 ataactaagg gcacaaaccg gtcgccgccg cgtacccggc cgaccagata cgcaccctgg
     1921 ttccgataga ataccggctt cataacctcg accgactcga tcggctgtgc gccgcaggcg
     1981 agccgccatt gcgactcgat ttcggcagcg accaatcgag catcgcgatc caggtcctga
     2041 tacggaatcg tgaatcggta atgcgcgagt atctcagcga tcagggtatg ggtcgaaccc
     2101 tgcccggcga acgtgatgta aaccggttct tgcgacacgg atgggggcgc gtcgaagtcc
     2161 gagtccacgt actcgatctt cggatcgacg cctatggtgg cgaagatgcg ccgcgtcacc
     2221 gagttgaaga aggtctcggc cagctcgaaa tcggcacggc gagcgatcca tgcggagtag
     2281 gtggtcttca tctgcgccca gagggcgcga tcgtgaaccc gctcggcgag aagcgagcga
     2341 atatccacga cgagcagggc gatgactcgc gcgtagacgt cgagccgctc caccgcgtca
     2401 cgctgcatgc cgtgccagtc ccgattctca aagcgagccg tcgcgcggcg cgtgatctcc
     2461 gcgaactgct gccggtaggc ctcgaacgcc cggctgatgg cgtcgaccgc gtaccgcacc
     2521 aataccgtgt catcgcgctc agacgacatg gcttgtgcct cactgcctcc caaaacgatt
     2581 agagattggc gatttcaaac ttccaatctc caatctctaa tctctaatca ccggtctcgg
     2641 aaaggtcaca tatgcgcgat caccgcatcc ccgaactcgc tgcatttcac ttctttcgcg
     2701 ccttccatca gccgcgcgaa gtcgtaggtg acggtcttgc tgccgatcgc gccgtccatg
     2761 cccttgataa cccgatcggc cgcttcggcc cagcccatgt agcgcagcat catctcgccc
     2821 gaaaggatga cggagccggg attgaccttg tcgagattgg catacttcgg cgccgtgccg
     2881 tgcgtcgctt cgaatacggc gtggccggtg acgtagttga tgttgccgcc cggcgcaatg
     2941 ccaataccgc ccacctgcgc cgccagcgcg tctgacaggt aatcgccgtt caggttcatc
     3001 gtcgcgatca cgtcgaactc gtccggccgg gtcagcacct gctgaagcgt gatgtcggca
     3061 atcgcatctt tgacgaggac cttgccccgg gccagctcgg ccttctgctc ggcgttggca
     3121 gcctcctcgc ctcgctgcgc gtccgccctg gtgcgctccc actgcgacca ggtgtacact
     3181 ctatcgccga attcgcgctc ggcgagtgcg tagccccagt tgcggaacgc gccttcggtg
     3241 aatttcatga tattgccttt gtggacgaag gttacactct tgcgcttgtt ggcgatggcg
     3301 taattgatcg ccgcgcggat caggcgctcg ctgccgtcgc gcgataccgg tttgatgccg
     3361 atgcccgacg tttccgggaa tcggatcttg ccaaactctt tgggaaaagc ttctttgaat
     3421 agcgcaagga atc
//
LOCUS       DBNHXB010000427         5261 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1304510, whole genome shotgun sequence.
ACCESSION   DBNHXB010000427 DBNHXB010000000
VERSION     DBNHXB010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1304510"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="AC162G_16800"
     CDS             complement(<1..569)
                     /locus_tag="AC162G_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016863742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077518.1"
                     /translation="MSDELYFNGIDGASGEYLLPPLTAKDLSALARGKGLDVEQIDEL
                     KARQRQESHYGVKEGVDPKDLSQTGWGVIFAHGADPAIREALSELLSHRQAQAGGHYR
                     EYAGVNAYRPNESKTAFLARHGAGPGPADPDKVPYYLLIAGDPQTVPYRFQYQLDVQY
                     AVGRIHFDSLEEYAQYARSVVDAEKGKIAL"
     gene            complement(615..773)
                     /locus_tag="AC162G_16805"
     CDS             complement(615..773)
                     /locus_tag="AC162G_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077519.1"
                     /translation="MTEAYTDLLLRIFPYDERAQSHPVEGELEDGSRFTGGQFKLDRQ
                     ALLALRFA"
     gene            1167..2162
                     /locus_tag="AC162G_16810"
     CDS             1167..2162
                     /locus_tag="AC162G_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017743633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HIR5077520.1"
                     /translation="MHWIVLSYSMPSGGRSGPRVTQWRRLRRLGVVTLAGGSHVLPAR
                     DECLEAFQWLAQEIRHAKGEAVVMRVQSVEGLTDRQIVDLFHAARGEEYAAIEADATA
                     LEKTARSKAGSRDPASLLDELSKLRRRYAEVARVDYFACPEGNRVAAHLARLEHLLSP
                     GTAPLEKVAPVRIADYRKRRWVTRPRPHVDRLACAWLIRRFVDPKAVIRYAARPEPGE
                     IAFDMDEGEFGHQGNLCSFEKMLLAFGLDDPALQMLAEIVHEIDLRDGRYARPEAAGV
                     EAILAGWLTAGLSDADLETNGIALFEGLCAAFSSHTLAMGSTRKKPKSKRSGGKS"
     gene            2159..2596
                     /locus_tag="AC162G_16815"
     CDS             2159..2596
                     /locus_tag="AC162G_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HIR5077521.1"
                     /translation="MKWVTRSHVHVDRVACPWLIRRFVDNEAEFLFVPNSQVDTVAAE
                     RGAIPFDAPGVELGHHEGRCSFESIVLKYDLRDPALKRLAKIVHAADIAEDIDTDPIA
                     RGLEAIASGFSLRYPDDEENLARQFEVYDALYAWCRLQTSRSA"
     gene            2597..3979
                     /locus_tag="AC162G_16820"
     CDS             2597..3979
                     /locus_tag="AC162G_16820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR5077522.1"
                     /translation="MAPQRFSRDQTVSIRTVPTMRPAPGITGLVLKLAWPAVIEQVLI
                     VGVDLTDLFIVGHLGASALSAVGLTAQVVLLAMAFFSAIGTGCMALIARHIGAGERAD
                     AGRILQQSILVAAGLSTAAGSVAWMFAPDILRSLGAEPDVVELGESFMRLIALSLPLM
                     AVTFVGNAALRAAGDTRTPMLLTGLHLAINAVLGLFLVYGPPRLNVNGLGLATSTARG
                     ALGLTVLGLLWRGHLGVRLTRDHWKLDLARIGRVLRVGLPAGVEQVLMQFALVNVTAV
                     IVGLGTVLYAAHNVSLRIMSLSYLPGWGFAVAASTLVGQNLGASDPGRARASVHAALR
                     LAFGVMVILGVALYALAAPIARIFTDDPDVIAASTSAIRVAAFSQPIMAVSFVFVHSL
                     RGAGDTRMVLAITATSIWTVRLGVAYLGAHVLGLGLAGAWLGILADFAARAFCGWLRF
                     RSGRWQTIRV"
     gene            4015..>5261
                     /locus_tag="AC162G_16825"
     CDS             4015..>5261
                     /locus_tag="AC162G_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5077523.1"
                     /translation="MSSSLSLSRALGILTSDSRLLLSTRIVRLFAYGFLSVVLVLYLA
                     QIGLSETQTGLLLTLALIGDTAISLWITTNADRIGRRRMLIAGAGLMLCAGVLFALTS
                     DFVLLLVAATIGVISPSGNEIGPFLPIEQAALAQTVPAARRTAVFAWYNLAGSMATAV
                     GALFGGVLAQALQEAGATPLNSYRAVLIGYAAIGLLLALLFMRLTDEVEANVSPTPHP
                     STRRLGLHRSRRVVLKLSGLFALDAFAGGLVITSIVAYWFTVRFGVEEATLGAIFFGA
                     NILAGVSALLAARIAARIGLINTMVVTHLPSNVLLCLVPLMPNLPLAIGVLLLRFSIS
                     QMDVPTRQSYTMAVVAPDERSAASGVTSIARSVGASLSPTMSGLFLASPVLLSAPFLV
                     AGGLKIVYDLLLYHSFRALKPPE"
BASE COUNT          864 a         1580 c         1706 g         1111 t
ORIGIN      
        1 agcgcgatct tgcccttttc ggcgtcgacg acgctgcgcg catactgtgc gtattcttcc
       61 agcgagtcga agtgaatgcg gccgacggca tactgcacgt cgagctggta ctgaaagcga
      121 taggggacgg tctgcggatc gccggcgatc aaaaggtaat aaggcacctt gtccggatcg
      181 gccgggccag gccctgcgcc gtgacgagcc agaaacgcgg ttttcgactc gttcggcctg
      241 tatgcgttaa cgccggcata ctcccggtag tgtcctccgg cttgagcctg gcggtgcgac
      301 agcaattcgc tgagcgcctc gcggatggcc ggatcggcgc catgcgcgaa gatgacgccc
      361 caaccggtct gcgacaaatc cttgggatcg acaccctcct tcacgccata gtgtgactcc
      421 tggcgttggc gcgctttcag ttcgtcaatc tgctcgacgt caaggccctt tccgcgagcc
      481 agagccgaca agtcctttgc cgtcagcgga ggcagcagat actcgccgct ggcgccgtcg
      541 atgccattga agtaaagctc atcgctcatt tcgtctcctc tctgaagtgc acgcgccttc
      601 agccgccgga ttgatcacgc gaagcggagc gcgagcaggg cctgacggtc gagcttgaac
      661 tggccccctg tgaagcgact cccgtcctcc agctctccct cgaccggatg cgactgtgcg
      721 cgctcgtcgt aagggaaaat gcgcagcagg aggtcagtat aggcctcggt catgacgatg
      781 tgctcctatc cgcataacct tgcgaaggtt gtgaaacctt cgcaagggtg acggatctcg
      841 attgagatgt ggagggtggg acagttagag cctatccgaa aaccaccgga gtgcggactt
      901 cagtccgctt cagatgcggc aggctgaagc ctgcactcca gttttcggat aggttcatag
      961 ggttgataag agcgaggggc gcatcggctg cgccacccgg cagttatcat acggcatgtc
     1021 aatcgagatt gcaagttccg gccggaggca ttcgtattcg gactgaacca gctccctgcg
     1081 aggacgtgag cctcagtacc cccgaatcat catgggtgta acaaatattg cttgctttag
     1141 aaatatgtgt tacaatccat cgtgccatgc actggatcgt cctatcctac tcgatgcctt
     1201 ccggcggccg atcggggccg cgcgtcacgc agtggcggcg cctgcggcga ctgggcgtgg
     1261 tcacgctcgc cggcggctcg cacgtgctgc ccgcacgcga cgagtgcctc gaagcctttc
     1321 agtggctggc gcaggaaatc cggcacgcga aaggtgaggc ggtggtcatg cgcgtgcaga
     1381 gcgtcgaggg cctgaccgat cggcagatcg tcgatctgtt ccatgcggca cgtggagagg
     1441 agtatgccgc gatcgaggcg gatgcgacgg ccttggagaa gaccgcgcgt tcaaaggccg
     1501 gctcaagaga cccggcttct ctgctcgacg agctaagcaa actgcgccgc cgctatgcgg
     1561 aggtggctcg cgtcgactac tttgcctgcc ccgagggaaa ccgggtcgcc gcccacctgg
     1621 cgcgcctgga gcatttgctg tcacccggta cagcgccgct tgaaaaggtc gcgccggtga
     1681 ggatcgccga ttaccgcaag cggcgctggg ttactcgtcc tcgcccgcac gtggatcgac
     1741 tggcatgtgc ctggctgatc cgcagattcg tcgatcctaa ggcggtaatt cgctacgccg
     1801 cgcgcccgga gcctggcgag atcgcattcg acatggacga gggcgagttc gggcatcagg
     1861 gcaatctatg ctcgttcgag aagatgctgc tcgcattcgg gttggacgat cccgcgctgc
     1921 agatgcttgc ggagatcgtc cacgagatcg atttgcgcga tgggcgttac gcacgaccgg
     1981 aggcggccgg cgtcgaggcg attctggccg gctggctgac ggccggcctg tccgacgccg
     2041 acctcgaaac gaacggcatt gcactattcg aagggttatg tgctgcattt tcgagtcata
     2101 cgcttgctat gggaagcacg aggaagaaac cgaaatccaa acgctcagga ggcaaatcat
     2161 gaagtgggtc acccgttcgc acgttcacgt cgatcgcgtc gcctgcccgt ggctgatcag
     2221 gcgcttcgtg gataacgagg ccgaattcct gttcgtcccg aacagccagg tggacacagt
     2281 cgccgccgaa agaggcgcga ttcccttcga cgcgccaggc gtcgagctgg ggcatcacga
     2341 ggggcgctgc tccttcgaat cgatcgtgct caaatacgat cttcgggacc cggcgttgaa
     2401 acggctggcg aagatcgtgc atgccgccga catcgccgaa gacatcgata ccgatcccat
     2461 cgcgcgcggg ctggaagcga tcgcgtccgg gttcagcctg cgctatcccg acgacgagga
     2521 aaacctggcg cgccagtttg aagtgtacga tgcgctgtat gcgtggtgcc gcctgcagac
     2581 gagcaggagc gcttgagtgg ctccacagcg attctcacgc gatcaaacgg tttccatcag
     2641 gactgtgccc accatgcggc ctgcgccggg tatcacgggg cttgtcttga agttggcttg
     2701 gccggcggtg attgagcaag tcttgatcgt cggtgtggac ctgaccgacc tgttcatcgt
     2761 cggccacctg ggcgcatctg cactgtccgc cgtcggattg acggcgcaag tcgtgctgct
     2821 ggcgatggct ttcttcagcg cgatcggaac cggctgcatg gcgctcatcg cacggcacat
     2881 cggcgcgggc gagcgcgccg atgcaggccg gatcttgcaa caatcgattc tcgtcgccgc
     2941 cgggctgagc acagccgccg gatcggtcgc gtggatgttc gcgccggata ttttgcgcag
     3001 cctgggcgcg gagccggacg tcgttgaact gggagaatcg ttcatgcgcc ttattgcgct
     3061 gtcgctgccg ctgatggcgg tcacgttcgt cggcaacgcg gccctgcgcg ctgctggcga
     3121 cacgcgcaca cccatgctat tgaccggcct gcatctggcg atcaatgccg tgctcggtct
     3181 ttttctggtc tacgggcctc cgcgcctgaa tgtgaatggg ctgggtcttg cgacgagcac
     3241 ggcgcgaggc gcgctgggtc taacggtgtt agggttgctg tggcgcggcc atctgggtgt
     3301 gcgactgacg cgagaccatt ggaagctcga cctggctcgc atcgggcgtg ttctccgcgt
     3361 gggcctgccg gccggtgtcg agcaggtgct gatgcaattc gcgctggtca acgtgacggc
     3421 ggtaatcgtc gggcttggga cggttctgta tgctgcgcac aacgtcagtt tgcgcatcat
     3481 gtcactgtcg tatttgccgg gttggggttt tgcggtggca gccagtacgt tggtcgggca
     3541 gaatctcggc gcgagcgatc cgggccgcgc gcgggcaagc gtgcatgctg cgctccgact
     3601 ggcattcggc gtgatggtga tcctgggtgt ggcgctatac gctttggctg cgccgatcgc
     3661 gcgtatcttc accgacgatc cggacgtcat cgccgcgagc acttccgcca ttcgcgtcgc
     3721 agcattctca cagcccatca tggcggtgtc gttcgtgttc gtgcattcgc tgcgtggcgc
     3781 gggcgacaca cgcatggtgc tcgcgatcac cgcaacctcg atttggacgg tgcggcttgg
     3841 cgtggcctat ctcggggcgc atgtgctggg cttggggttg gccggcgcct ggctaggcat
     3901 tctggccgac ttcgccgcgc gcgcgttctg cggctggctt cgctttcgca gcgggagatg
     3961 gcagacgatt cgcgtctgat tctcgaagaa tgcccactcc cctggaatga aagcatgtca
     4021 tcgagtctct ccctctcccg ggctttgggt atcctgacat ccgatagccg tcttttgttg
     4081 agcacgcgga tcgttcgtct gttcgcgtat gggttcttgt ccgtcgtcct ggtgctgtat
     4141 cttgcccaga tcggattgag tgagacgcag acagggttgc tgctgacgtt ggcgctgatc
     4201 ggcgacacgg cgatctcgct gtggatcacg acgaacgccg atcggattgg ccggcggcgc
     4261 atgctgatcg ctggcgcggg cttgatgctg tgtgcgggtg tgttgttcgc gctgacgagc
     4321 gacttcgtgc tgctgctggt ggccgcgacg atcggcgtga tcagccccag cggcaacgag
     4381 atcggcccgt tcttgccgat cgagcaggca gcgctcgcgc agacggtgcc ggccgcgcgg
     4441 cgcacagcgg tctttgcctg gtataacctg gccggctcga tggcgacggc agtgggcgca
     4501 ctgttcggcg gtgtgctggc gcaggctctg caagaggcgg gcgcgacccc tttgaatagt
     4561 taccgcgccg tcctgatcgg ttacgccgcg atcgggctgc tgctcgcact actattcatg
     4621 cgcctgacgg atgaggtcga ggcgaacgtt tcgccgacgc ctcatccatc gacccgccgc
     4681 ctgggcctgc accgctcgcg ccgcgtcgtc ctaaaactct cgggcctgtt cgcattggac
     4741 gcgttcgcgg gcgggcttgt catcaccagc atcgtcgcct actggttcac cgtccgcttc
     4801 ggcgtggaag aggcgaccct gggcgcgatc ttcttcggcg caaacatcct cgccggggtt
     4861 tcggcgctgc tggcggcccg tatcgcggca cgaatcgggt tgattaacac catggtggtt
     4921 acccatctgc catcaaacgt gttattgtgt ctcgtgccgt taatgcccaa cctgccgctg
     4981 gcaatcggtg tgctgttgtt gcggtttagc atctcgcaaa tggacgttcc aactcgccaa
     5041 tcctatacga tggctgtggt tgcccccgac gaacgctccg ccgcttcagg tgtaacgtcg
     5101 attgcccgct ctgttggcgc gtctctatct cctacgatga gcggcctctt tctcgccagc
     5161 ccggtactcc tgagcgcgcc gttcttggtc gcgggtggcc tgaaaatcgt ttacgacttg
     5221 ctcttgtatc acagtttcag agcacttaaa ccacccgaag a
//
LOCUS       DBNHXB010000428        17649 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1135986, whole genome shotgun sequence.
ACCESSION   DBNHXB010000428 DBNHXB010000000
VERSION     DBNHXB010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 17649)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17649)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17649
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1135986"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="AC162G_16830"
     CDS             <1..222
                     /locus_tag="AC162G_16830"
                     /inference="COORDINATES: protein motif:HMM:NF014037.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HIR5077524.1"
                     /translation="QAAVGPPPDGALTRCAVCNARLEPIAKAGVEDRVPPYVFQTQGE
                     FYHCPQCDRVYWAATHVERMRKMMEGLDG"
     gene            290..1063
                     /gene="tpiA"
                     /locus_tag="AC162G_16835"
     CDS             290..1063
                     /gene="tpiA"
                     /locus_tag="AC162G_16835"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR5077525.1"
                     /translation="MRVPILAGNWKMNKTVDEAIDLVRELRRLVLDVEGVEIVVCPAF
                     VALASVAGALKGTKLKVGAQDMHWEEKGAFTGEVSPMMLEGLCDYVIIGHSERRQYFG
                     ETDESVNRKVKAALAHGLTPIMAVGENLQQNEAGLTAEVVSSQVHRGLEGVTAEQARS
                     IVIAYEPVWAIGTGKAATGAGANSVVAVSIRGTLADLYGEDVAQSIRVQYGGSVTPAN
                     IAEFMVMPDLDGALVGGASLKAGDFSEIVKITAKVKRGA"
     gene            1064..1846
                     /locus_tag="AC162G_16840"
     CDS             1064..1846
                     /locus_tag="AC162G_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077526.1"
                     /translation="MDAVIWFIATLVLLVVVVQWLHRHILGFFFLITGNEDMALVLYF
                     LPLTPGVLLHELSHAITAFILRVRAGGFTVFPRRDSSGRITLGSVMVEQVDPIRSSLI
                     GVAPLVFGCSAVLLIGYRVFGLSEMGSVLLAGDFQAIAATAGRLFQTPDAWLWLYLTF
                     AISNTMLPSASDRETWPSVILFIVGVVALVLLANQTAIVEALALPLGAAINWLTAAFV
                     ITLAIDLPVAVLLGLLEWGTGSIAGRRVQYSKPGDDKPKRRR"
     gene            complement(1876..2931)
                     /locus_tag="AC162G_16845"
     CDS             complement(1876..2931)
                     /locus_tag="AC162G_16845"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0070085 - obsolete glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5077527.1"
                     /translation="MTPFLLTFLIAFALSLLLTPLAHRLGLRLGIVDAPGGRHKHTGM
                     VPRLGGMPLYVAFTVAVLASRSMAVRSFDPQEPIRIAGLLIGGTFVFVYGLIDDRYNL
                     RPVWHVAAQFVASLIAIASLIIIERFNNPLTNVLVILEAWQYVPLTIFWMMGMMNTVN
                     WLDGLDGLAAGIAAIFSAVLFVAMIRTTPEQPQPQLSIALLPMALLGATLGFLPFNFY
                     PARVFLGSGALYLGYVLGSLGIIGGAKVATVLLILAVPILDVAWLIVSRVRRGQSPAR
                     GGRDHLHFRLLDMGLSQRTIVALYYAVSAAFGVLALLIEGRLIKLITLGLLGTITIAA
                     LALLSRNTGGRQTTADD"
     gene            complement(2972..3145)
                     /locus_tag="AC162G_16850"
     CDS             complement(2972..3145)
                     /locus_tag="AC162G_16850"
                     /inference="COORDINATES: protein motif:HMM:NF024759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR5077528.1"
                     /translation="MKKSSFVKTELDRITYQIIGIAMDVHNQLGSGHREEAYHNALVA
                     SASPNAPKVTTRK"
     gene            complement(3247..4218)
                     /locus_tag="AC162G_16855"
     CDS             complement(3247..4218)
                     /locus_tag="AC162G_16855"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR5077529.1"
                     /translation="MSASPVTLVDLHAARARIAPHILHTPLTHSYLLSDQIGCEIWLK
                     QENRQHTGSFKPRGALNKIASLSDEERSRGIVAASAGNHALGVAHACRVLGVERAHVF
                     VQATAAPAKIAKLRQYPVRLHLAGDTFEEAQQAALAHSRETGGTFVSAYDDPAVLAGQ
                     GTCGLEIVEDSGGLDAVIVPVGGGALIGGIALAVKAAHPATRVVGVNPAASPSALLSF
                     QRGHAIDPYDHEPTLAHGLAGGFGKIPFAVARNLVDEIVLVTEDEMKQAIAALIDSDQ
                     ILAEASGVAALAAVLFRKTAAWGKVVVVISGGNIDSGTLRSILAGKS"
     gene            complement(4220..4846)
                     /gene="upp"
                     /locus_tag="AC162G_16860"
     CDS             complement(4220..4846)
                     /gene="upp"
                     /locus_tag="AC162G_16860"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR5077530.1"
                     /translation="MALLHVSTHPLVRHKIALLRDTRTDPKKFRELVREIAILLCYEA
                     TADLALEESSVTTPMGQAVGHRLREQIGLVPVLRAGLGMVEGIWEMMPAAEVWHIGLF
                     RDERTLKPIEYYRKLPVSPTVDVCLILDPMLATGGSASATAKILKDWGAKRIKYVGII
                     AAPEGVKKLSTDHPDVPIHVGAVDDHLNDIGYIVPGLGDAGDRQFGTG"
     gene            5070..6092
                     /gene="ruvB"
                     /locus_tag="AC162G_16865"
     CDS             5070..6092
                     /gene="ruvB"
                     /locus_tag="AC162G_16865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR5077531.1"
                     /translation="MIDRSVSPHQREEEAALDSALRPTRLADFPGQERLKQNLSILIE
                     AAKARGEVLDHVLFYGPPGLGKTTLSRIIANEMGVNVRVTAGPAIERAGDLVAVLTNL
                     KAGDVLFIDEIHRLGRAIEEVLYPAMEDFAVDIILGKGPSARNIRLGLPHFTVVGATT
                     RLALMTAPLRARFGAVYRLDYYDQAIMEQIIRRAAAVLGLETDDEGIAEIARRSRGTP
                     RVGLRLLRRVRDYAQVRGDGLVTGALAAEALALLDVDVLGLDDLDRRVLRSIVEKVNG
                     GPVGLDTIAASISEEADTIMDVVEPYLLQLGFLDRTPRGRVATQRAYEHLGLPYPERP
                     QQPSLL"
     gene            6101..8341
                     /locus_tag="AC162G_16870"
     CDS             6101..8341
                     /locus_tag="AC162G_16870"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HIR5077532.1"
                     /translation="MADLTGQRLGQYLILERISKGATSTVYKAYQEKLERYVAVKVLS
                     PHFIDEEGFLERFKQEARAVAQLDHPNILPVYDFDQVGDVVYIVMKYVDTGTLKDLLG
                     APMDLRATLEIVTQVGLALGFAHRQGVIHRDVKPGNILLGEGNWALLTDFGLAKMRAG
                     DRKLTKSGIGLGTPDYMAPEQGQGLPVDGRADLYSLGCMLHEMVTGRVPFEADSGMAV
                     VVKHITEPPRPPREINPRLPLAVEHVILRALEKDPSRRYQTAESMVADLARAIGSADQ
                     FMLLEELPAPAPARGQPRPTVRVADTRPAFPSPAPIVQMRERLGKHRPAVGAGIKSAN
                     VWLALRLRHVRQAARAVLERFWQGLRRLPVIAVAFVKRGHAGVSTALPARAPASSRSA
                     TSRLNTSAVRTKSARARTGAASKQRKAGARPSSVPSMARFANRIRLAFEAARERLSRH
                     RRLAAVAVLALLILLLALWLAGGSAGGAVPSQTARATDTAPGTPVVTVPGAGTPAASP
                     VPDAPAGMLLVPGGTFKMGATGGHFDPDETPPHRVTVDPFYIDAVEVTVAQFARFASA
                     WGDVTDAEKKGDATTWRAANTPDRQRFPVNRVSWNDAAAFCRWYGKRLPTEAEWELAA
                     KGFTENIYSWGNAFDRNRANTQERGVGQPIAVASLPNSGPFGAYDMIGNVWEWVADWY
                     GGDYYGVSPSVNPQGPASGLERVVRGGSFKSDGERATTTIRRKASQDGWSDDIGFRCV
                     KDVSSP"
     gene            8421..8645
                     /locus_tag="AC162G_16875"
     CDS             8421..8645
                     /locus_tag="AC162G_16875"
                     /inference="COORDINATES: protein motif:HMM:NF022596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HIR5077533.1"
                     /translation="MSDLGKWLVLIGLSIAALGGLVWLLGRLPFFGNLPGDIRIQGEN
                     LGCFVPLVSMLLLSLLLTVILNVVIRLLNR"
     gene            8648..9682
                     /gene="queA"
                     /locus_tag="AC162G_16880"
     CDS             8648..9682
                     /gene="queA"
                     /locus_tag="AC162G_16880"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR5077534.1"
                     /translation="MKTFELDYDIPPELIAQTPIEPRDAARLLVLDRRSGALAHRRFR
                     DVVEYLRRGDLLVFNETRVLNARLQAVKIPAGGRVEVLLLKRLDARTWEGLVGGSGVR
                     VGSRLRILPASEPASNGVAVKVIAEFERGGRVLRFDRPIDDQLDMLGSVPLPPYIRQP
                     LHDSERYQTIFARLPGSVAAPTAGLHFTPGLVQTLREHGVELAFVELRVSLDTFRPIL
                     EEEVEQHAIHTEYCRVSTETVEHIERARHAGGRVIAVGTTAVRTLETASQADGRVAPF
                     DGDTSLFITAGYRFRVIDAMITNFHLPRSTLLALVAAFAGLEQLLAAYRVAIAERYRF
                     YSFGDAMLIL"
     gene            9874..10827
                     /locus_tag="AC162G_16885"
     CDS             9874..10827
                     /locus_tag="AC162G_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HIR5077535.1"
                     /translation="MQAEEARTVLGAGLDEYLASLAHEKRYSRNTIAAYRNDLGQFVD
                     HVVVLNAVPGGRWAGMPPNVVGDYVARLKEMTWRDPSGREKRMATSTVARKIAALKSF
                     FRYLARIGQIDSDPTLGLEAPKVKKRLPRTLSSAEVERLLAAPGQSSQPKALRDRALL
                     ETLYATGMRVSELVSLNLDDVNLVTHDVRVRNDRGDKERIVPMPDRAVAALQMYLERG
                     RPALVKNTKDSNALFLNHRGQKLTRQGMWLIIKEYAEQAGIDFDVTPHTLRHSFAAHM
                     LENNKASLGEVQHFLGHASVSTTQIYSQLAVEPKPDPVAGE"
     gene            10883..11521
                     /locus_tag="AC162G_16890"
     CDS             10883..11521
                     /locus_tag="AC162G_16890"
                     /inference="COORDINATES: protein motif:HMM:NF015648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR5077536.1"
                     /translation="MGYVEQLLAKGEEIVLRARQHWVTLIASALANALFVIVVLVLYF
                     VVRSIEFLKPAETVLLVAALIGVLFGLARYSWDWLRWWSEEYIVTSRRVIQTEGIVNK
                     RTADSSLEKVNDVLLTQSFFGRILGYGDLEIVTGSDVGVNRLTRLRDPINFKTAMLDA
                     KARLGEEYGEQMREVTDRDVPKLIEELDALRKRGLITDAEFNEKKARLLSQL"
     gene            11524..12369
                     /locus_tag="AC162G_16895"
     CDS             11524..12369
                     /locus_tag="AC162G_16895"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR5077537.1"
                     /translation="MDDFVTRPQVDEAVAAIRDRIHTRPRAGVVLGSGLGALVDAVES
                     PVTIPYADVPHWPRSSVEGHAGRLVSGKLEGVDALVMQGRTHHYEGHSMGRVTFPIRV
                     MQFLGIDTLIVTNAAGGLNLSFRAGDVMLIQDHINLIGMAGQNPLRGPNDSSFGTRFP
                     DMSQAYDSALRALAQRVAREQGIELREGVYICLGGPSFETPADVRFLRLIGADAVGMS
                     TVPEVTVARHGGLRVLGLSGISNVLPPEGVTHTTTHEEVLEAGRTIVPRMLTIVRGVL
                     RALRA"
     gene            12377..13147
                     /locus_tag="AC162G_16900"
     CDS             12377..13147
                     /locus_tag="AC162G_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077538.1"
                     /translation="MAAFVRVIEQFAVVLYTVSLAGVAWSLRSAWLAMRESRETLYSL
                     EREAATARMGRGLLSALGFVALGVTVFVVAQVVAPQLPASEAPPPPPGPLVTLTPTVT
                     PFPTPTPISSPTPAPSPNATILLVETIETSTPAPTPVPLPPEACPDPNVRITAPGDGT
                     VLAGAFQVFGTANIENFAFYKFVLNGPATNFEDRTAGDVYEAPVADSYLGTLDESLVT
                     ILLQSPGAYRFKLIAVNNEGNEAPACTITLQFLPSVPR"
     gene            13284..13823
                     /locus_tag="AC162G_16905"
     CDS             13284..13823
                     /locus_tag="AC162G_16905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5077539.1"
                     /translation="MSGLTIRPALMGDMQECLEFDASYATEYVWQMESHASSGEISVS
                     FRHVRLPRSMRVEYPRDAEALLAEWRLRDTLLVAERDRVKLGYVSLGLQPGQRAAQIG
                     DLVVGRLYRRTGVGSALIGAALRWARDRGLKQVVIEVQTKNHPAISLLNKLGFTFCGF
                     NDRHYLNRDIALFFSRRVR"
     gene            13875..15830
                     /locus_tag="AC162G_16910"
     CDS             13875..15830
                     /locus_tag="AC162G_16910"
                     /inference="COORDINATES: protein motif:HMM:NF028237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HIR5077540.1"
                     /translation="MTLTTIFIFINTLIATGIAITAFSLMLYVVRYNWSSPVARAFVL
                     ILAGTMIVFTGDAVLPRVTSIGAAEGWLRFEWIGIALAPAAYLHFSDEVLRTTNAVSL
                     RRRRGVYLGYAYAVVFAIAALATDWLVQPGVTSLVTPRLSPGPFFPVYIAYFAVAVGF
                     GFRNIVVARARCLTPTSRRRMTYLLLSSTAPSLGVFPFLLVVGQGGAEIGPGFWLLVI
                     VGNATIGLMLLLMAYSVAFFGVLAPDRVVKRRLIRFLLRGPVTAGAVVTAMLVFTRLE
                     SLLNLPRETLMVGAVVISVVAFAVLRGSFQPLIDLLLFQQDQDELAYIEQLGERLLTT
                     TDLQQFLENVLTSVADLLRTPTAFIASRRASDRWEMLAIIGAVQPVERVLGELQSGAQ
                     TTKSAGGPHRADGFWTWPLRSRGDGELIGALAVAGGGAPPELGNDERKQLDVLMRQAA
                     IALDDRRLQEEVFAAVEQILPEIDVIQRQRGAQRYLGSPPQPPLEAALGTDPEFVTHV
                     KDALSHMWGGPKLTTSPLLRMAVVDRMLPSHNGNAPKALRAVLVDTIELLRPDGPRSL
                     TAAEWMLYNILELRFLRGERVRDIARKMAMSESDLYRKQRVAVGAVAQVLAQMEQSAL
                     ETTGPPAGEPVGLTPVNGNRGGRENTA"
     gene            16072..17031
                     /locus_tag="AC162G_16915"
     CDS             16072..17031
                     /locus_tag="AC162G_16915"
                     /inference="COORDINATES: protein motif:HMM:NF012784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR5077541.1"
                     /translation="MAHEAPQDLSCDEAYWESLLRDVETQAPGPAGDHFRAPAAAPRA
                     DAAGTAWKAAQRASENDEVIQVQSVGSNRGGLLIEWNGLRGFLPASHLCGLAAHLDDE
                     GRRREIVQRVGRTFNAKVIEIDRGQGRFVVSERLACTQHDRRAALLAELESGQVRSGV
                     ITSVCSFGAFVDLGGVEGLLHISEISWGRVNHPGDCLKAGQSVRALVMSVDRENERVA
                     LSVKRLYPDPWMTVSERYSVGQIVQCVVTSVVAFGAFARLEDGLEGLIHVSELAEGNF
                     LHPRNVVCEGQTVRVRVLNVDAAHRRLGLSLRRVDGDLLERAT"
     gene            17049..>17649
                     /locus_tag="AC162G_16920"
     CDS             17049..>17649
                     /locus_tag="AC162G_16920"
                     /inference="COORDINATES: protein motif:HMM:NF024882.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HIR5077542.1"
                     /translation="MARKHRARDKAKPGKSAQHRPEPRRRQLRIAFGARRQEVAGVLL
                     IVLAAISLLALAGITTGFLIDWWSVLLKRVFGVGAYVVAVSFGLAGYFIILRAQGKVS
                     RIQWLRLIGLEGAFFAALAALHAAAWWADSWALIDQGTGGGIVGWGLSTALVDSVGRA
                     PTVLIFAAVAFGALSLALKIDWWALVDQGRNRLSTRPVRP"
BASE COUNT         2985 a         5421 c         5860 g         3383 t
ORIGIN      
        1 caggcggccg tcggtccacc gccggatggc gcgctcaccc ggtgcgcggt gtgcaacgcg
       61 aggctcgaac cgatagccaa ggccggcgtc gaagaccgtg tgccgccgta cgtgttccag
      121 acacaagggg agttctacca ctgcccacag tgcgatcggg tttactgggc ggcgacgcac
      181 gttgagcgga tgaggaagat gatggagggg ttggatggtt gacgaccggc actttccaag
      241 cgccggtcgt gttattgtat aatctcgcta ttcccactgg aggtgctcta tgcgcgtgcc
      301 gattctggcc ggcaactgga agatgaacaa aacggtggac gaggcgatcg acctcgtgcg
      361 tgaactacgc cggctggtgt tggacgtcga gggagtggag atcgtcgtgt gtccggcgtt
      421 cgtcgcgctg gcgagtgtcg ccggtgcgct caaagggacg aagctcaagg tcggcgcgca
      481 ggatatgcat tgggaggaga aaggcgcgtt caccggcgag gtgtctccga tgatgctgga
      541 gggtctgtgc gattacgtga tcatcggcca cagtgagcgg cggcagtatt tcggcgagac
      601 ggatgagtcg gtcaatcgca aggtcaaggc agcgctggcg cacggcctga cgccgatcat
      661 ggcggtcggg gagaacctgc agcaaaacga ggccggcctg actgccgaag tcgtcagctc
      721 gcaagtccac cggggtctgg aaggtgtgac ggcggaacag gcgcgcagca tcgtcatcgc
      781 ctacgagccg gtctgggcga tcggcaccgg gaaggcggcg accggggccg gcgcgaacag
      841 cgtggtagcc gtgtcgatcc gtggcacgct ggcagacctg tatggcgagg acgtcgcgca
      901 gtcgatccgc gtgcagtacg gcggcagcgt cacgcctgcc aacatcgccg aattcatggt
      961 catgccggat ctagacggcg cgctggtcgg cggtgcgtcg ctcaaggcag gcgatttctc
     1021 ggaaatcgtg aagattacgg cgaaggtgaa gcgcggggcg tgaatggacg cggttatctg
     1081 gttcattgcc acgctcgtcc tgctcgtggt cgtggtgcaa tggcttcacc gccacatcct
     1141 cggattcttc ttcttgatca cgggcaacga ggatatggcc ctggtcctct attttctccc
     1201 gctgacgccc ggcgtgctgc tgcacgagct gagccatgcc atcacggctt tcatcctccg
     1261 cgtgcgtgcg ggcggcttca ccgtattccc ccgccgcgat tcaagcggcc gcatcacgct
     1321 tggctcggtg atggtcgagc aagtcgatcc gatacgctct agcctgatcg gcgtggcgcc
     1381 gctggtgttc ggatgcagcg cggtgctgtt gatcggatac cgtgtcttcg ggctctctga
     1441 aatggggagc gtgcttcttg cgggtgactt ccaggccatc gccgccacgg ccggccgcct
     1501 ctttcagacg cccgatgcct ggctctggct gtacctgacc ttcgcgatca gcaataccat
     1561 gctgccaagc gcgagcgacc gcgagacgtg gccttccgtc atcctgttca tcgtaggcgt
     1621 cgtggccctc gtcctccttg cgaatcagac tgctatcgtg gaggcgctcg ctctgccgct
     1681 cggcgcagct atcaactggc tgacggcggc gtttgtcatc acgctggcga tcgatctgcc
     1741 cgtggccgtg ttgctgggct tgctggaatg gggaacgggg tcgattgccg ggcgccgcgt
     1801 gcagtactca aagccgggtg acgacaagcc gaagaggcgc aggtaaatac ccggctcgaa
     1861 cgaagggtgg ccgcatcagt cgtccgccgt ggtttgcctg cctcctgtgt tgcgtgaaag
     1921 cagagcgagg gctgcgatcg tgatcgtccc taacagcccc agcgtgatga gcttgatcag
     1981 tcgcccctcg atcagcagcg ccaggacgcc gaaggcggcg ctcaccgcgt agtatagtgc
     2041 gacgatcgtg cgctgtgaca gccccatatc gagtagacgg aagtgcagat gatcgcgccc
     2101 gccccgcgct ggcgattggc ctcgccgtac gcggctcacg atgagccagg ccacgtcgag
     2161 gatcggcacg gccaggatga gcagcaccgt cgccacctta gccccgccaa tgattccgag
     2221 acttcccagc acgtagccca agtacagcgc gcctgacccc aggaagactc gcgccggata
     2281 gaagttgaac ggcagaaatc ccagcgtcgc gccgagcaac gccatcggca gcaaggcgat
     2341 gctcaactgg ggctgcggct gctcgggtgt ggtgcggatc atggcgacga acaacacggc
     2401 gctgaatatc gcggcgatgc ccgccgccaa tccgtccaat ccgtcgagcc agttgacggt
     2461 gttcatcatg cccatcatcc agaagatggt gagcgggacg tactgccacg cttcgagtat
     2521 gaccagcacg ttcgtgagag ggttgttgaa ccgctcgatg ataatcaggg aagcgatggc
     2581 gatcagcgag gcgacgaatt gcgccgcgac gtgccacaca ggccgcagat tgtagcgatc
     2641 gtcgatcagg ccgtagacga atacgaacgt gccgccgatc aggagtccgg cgatgcggat
     2701 cggctcctgc ggatcgaagc tgcgcacggc catcgaccgc gaggcgagca cggcgaccgt
     2761 gaaggcgacg tacagcggca tcccgcccaa tcgcgggacc atccctgtgt gtttgtgtcg
     2821 cccgccgggt gcgtcgacga tgccgagtcg cagccccagc cggtgggcga ggggggtgag
     2881 gagcagcgag agggcgaagg cgatcaggaa ggtgaggagg aaaggtgtca tcgaatgggc
     2941 aacgaatcaa cgaatgggtc gcgtgcgcct ctcactttcg agttgttacc ttgggcgcgt
     3001 ttgggcttgc gctggcgacg agcgcgttat gatatgcctc ctcgcggtga ccggagccaa
     3061 gctgattgtg aacgtccatc gcgatgccaa tgatctgata agtgattcga tcaagctcgg
     3121 tcttgacgaa ggatgatttc ttcatgctcc tccccccttc atcgctccag aatgtgccaa
     3181 gcgcctgtta tcgcaaggcc tgcattattc gttgattcgt tgcatattcg ttgacaccat
     3241 ctcatctcac gacttgcctg cgagaatgct ccgaagcgtt cccgaatcga tattcccacc
     3301 cgaaatcaca accacgacct tgccccacgc cgccgttttg cgaaacagca ccgccgccag
     3361 tgccgcgacg cctgaggctt ccgccaggat ttgatccgaa tcaatcaggg ccgcaatggc
     3421 ttgcttcatc tcgtcttctg tgaccagcac gatctcgtcc acgagattgc gggccaccgc
     3481 gaacggaatt ttgccgaagc cgccggccag cccgtgcgcc agcgtcggct cgtggtcgta
     3541 gggatcgatg gcatggccgc gctggaacga aagcagcgcc gacggcgagg cagcggggtt
     3601 gacgccgacg acgcgcgtcg ccgggtgggc ggccttcacg gcaagcgcga tgccgccgat
     3661 cagcgccccg ccgccgaccg gcacaataac cgcgtcgagg ccgcctgagt cttcgacgat
     3721 ctccagtccg cacgtcccct ggcctgcgag gactgccgga tcgtcatacg ccgacacgaa
     3781 cgtcccgccg gtctcgcggc tatgggcgag cgctgcctgc tgcgcctctt cgaaggtgtc
     3841 gccggcgaga tgtagccgca ccgggtactg gcgcagcttg gcgatcttgg ccggcgcggc
     3901 agtcgcctgg acgaacacgt gcgcgcgctc gacgccgagc acgcggcagg cgtgggccac
     3961 acccagcgcg tgatttcctg ccgacgcggc cacaatgccg cgtgagcgtt cttcatcact
     4021 gagcgacgcg atcttgttca gcgcgccgcg cggcttgaag ctgcccgtgt gctggcggtt
     4081 ttcttgcttc agccatatct cacatccgat ctggtcactc agcaagtaag aatgggtcag
     4141 gggtgtatga agaatgtgcg gcgcgatgcg agcgcgcgct gcgtgtaggt ccacaagggt
     4201 gacgggtgag gcactcatct tatcccgtgc cgaactggcg atcgcccgcg tcgcccaggc
     4261 ctggcacaat gtagccgatg tcattgaggt ggtcgtcgac cgcgccgacg tgtatcggca
     4321 cgtcgggatg atcggtcgag agtttcttca cgccctccgg cgcggcgatg atcccgacgt
     4381 acttgatgcg cttcgcgccc cagtccttga gaatctttgc cgtggcagac gccgaaccgc
     4441 ccgtcgccag catcggatcg aggatcaggc atacgtccac cgtcgggctg accggtagct
     4501 tgcgatagta ttcgatcggc ttgagcgtgc gttcgtcccg aaacaggccg atgtgccaga
     4561 cttcagccgc cggcatcatt tcccagatac cttcgaccat gcccagcccg gcgcgcagca
     4621 ccggcacaag gccgatctgc tcacgcaggc gatggccgac ggcttggccc atcggcgtcg
     4681 tcacgctgct ctcttccagt gcgagatcgg cggtggcttc gtaacagagc aggatggcga
     4741 tctctcgcac cagttcacgg aacttcttcg ggtcggttct cgtgtcgcgc aacagcgcga
     4801 tcttgtgccg caccagcgga tgcgtgctga cgtggagcag agccatgttg agatctccgt
     4861 aggtgggatt gtcaggcatt gtaacgtcgc gtggcacgct tgtcaaagcc atggttgggt
     4921 tcccggccgc catccggccg aaggggcacc gcgagccgca acgctgttgt aaccggcctg
     4981 caagcctgtg accggacgcc gtgtgttacc atattcgtgg ctggcccagg tgttgcctgt
     5041 ggcgatgcat cgggtataat cgcatcgcca tgatcgatcg aagcgtatca cctcaccagc
     5101 gcgaagagga agcggcactc gatagcgcgc tacgcccgac ccgtctcgcc gatttccccg
     5161 gtcaggagcg cttgaagcag aatctgtcga tcttgatcga agcggcgaag gcgcgcggcg
     5221 aagtgctcga tcacgtgctg ttctacggtc cacccggcct gggcaagacg acgctatcgc
     5281 gcatcatcgc caacgagatg ggcgtaaacg tgcgcgtcac ggccggcccg gccatcgagc
     5341 gggcgggtga cctggtcgcg gtgctgacca atctcaaggc tggcgacgtg ctgttcatcg
     5401 acgagattca ccgtctgggc cgcgcgatcg aggaagtgct gtatccggcg atggaggatt
     5461 tcgcagtaga catcattttg ggtaaagggc cgtctgcgcg caacatccgt ttgggcctgc
     5521 cgcatttcac tgtcgtcggc gcgacgacgc gcctggcgct gatgaccgcg ccactgcgcg
     5581 cccgcttcgg cgcggtgtac cgcctggact actatgacca ggcgatcatg gagcagatca
     5641 tccgccgcgc ggctgccgtg ttgggcctgg agaccgacga cgaagggatc gccgagatcg
     5701 cccgccgcag ccggggcacg ccgcgcgtcg gcctgcggct cctgcgccgc gtgcgcgact
     5761 acgctcaggt gcgcggcgac ggcctcgtca cgggcgcgct ggcggccgag gcgctggcgc
     5821 tgctcgacgt cgacgttctg ggcctggacg atctcgaccg tcgcgtgctg cgctccatcg
     5881 tcgagaaagt caacggcggg ccggtcggcc tggacacgat tgccgcatcg atcagcgagg
     5941 aggccgacac gatcatggac gtcgtcgagc cgtatctgct tcagctcgga ttcctcgacc
     6001 gcacgccgcg cgggcgtgtg gcgacgcagc gcgcctacga gcacctgggc ttgccttatc
     6061 cagagcggcc gcagcaaccc agcctgttgt aggacggagc atggcagacc tcaccggtca
     6121 acgtctcggt caatatctga ttctcgaacg gatcagcaag ggcgctacct ccaccgtcta
     6181 caaagcgtac caggagaagc tggagcgcta cgtcgccgtc aaggttctct cgcctcactt
     6241 tatcgacgag gaaggtttcc tcgaacgctt caagcaggaa gcgcgcgccg tcgcgcagct
     6301 cgatcacccc aacatcctcc cggtttacga tttcgaccag gttggcgacg tcgtgtacat
     6361 cgtcatgaag tacgtcgaca ccggcacgct caaggatctg ctcggcgcgc cgatggactt
     6421 gcgcgctacg ttggagatcg tgacgcaggt gggcctggcg ctgggcttcg cgcatcgcca
     6481 gggggtcatc catcgtgacg tgaagcccgg caacatcctt ctgggcgaag gcaattgggc
     6541 gctgctcacg gacttcggcc tggccaagat gcgcgccggc gatcgcaagc tgaccaagag
     6601 cggcatcggc ctgggcacgc cggattacat ggcccctgag caaggccagg gcttgccggt
     6661 cgacggccgc gccgatctgt attcgctcgg ctgcatgctg cacgagatgg tcacaggcag
     6721 ggttcccttc gaagccgata gcggcatggc cgtcgtcgtg aagcacatca ccgagccgcc
     6781 gcgcccgccc cgtgaaatca accccaggct gccgctcgcc gtcgagcacg tcatcttgcg
     6841 cgcgctggag aaagatccga gccggcgcta tcagacggcc gagtcgatgg tggccgacct
     6901 ggctcgcgcg attggcagcg ccgatcagtt catgttgctc gaggagcttc ctgcgccagc
     6961 cccggcgcgc gggcagcctc gcccgactgt acgggtcgcc gacacgaggc ctgcgtttcc
     7021 cagtccggcc ccgatcgtgc agatgcgtga gcgactgggg aagcaccgcc cggcggtcgg
     7081 agcgggtatc aagagcgcaa acgtctggct cgcgctccgt ctgcgccatg tgcgccaggc
     7141 ggccagagcc gttctggagc gattctggca ggggctgcga agactgcctg tcatagccgt
     7201 ggcgttcgtc aagcgcggcc acgcgggcgt atcgaccgca ttgccggcgc gagcgcctgc
     7261 gtcgtcacgc tcggcgactt cacgattgaa tacctctgcg gtcaggacaa agtctgctcg
     7321 tgccaggaca ggcgcggcat cgaagcagag aaaggctggc gccaggccca gttcggttcc
     7381 atcgatggcg cggtttgcca accgaatccg gctcgcgttt gaagcggcac gcgaaaggtt
     7441 gtcgcgtcac cgccgtctcg cggctgtagc cgtgctcgcg ctgctaattc tactcctggc
     7501 attgtggctc gcaggcggtt cggccggggg cgctgtcccg agccagacgg cgagggctac
     7561 ggacactgca cccggtacgc cggtggtgac agtgccaggc gccggcacgc cggccgcgtc
     7621 gcccgttccc gatgctccgg caggcatgct cctggttccg ggcggcacgt tcaaaatggg
     7681 cgcgaccggc ggccatttcg atcccgacga gacgccgccg catcgagtca ccgtcgatcc
     7741 cttctacatt gacgccgtcg aggtgacggt cgcgcagttc gcgcggttcg cctcggcctg
     7801 gggcgatgtc accgatgcgg agaagaaggg cgacgcgacg acttggcgag cggcgaacac
     7861 gcccgatcgg cagcgctttc ccgtcaatcg cgtgtcatgg aacgacgcgg ccgccttctg
     7921 ccgctggtac ggcaaacgcc ttccgaccga agccgagtgg gagctggcgg cgaagggttt
     7981 caccgagaac atctattcgt ggggcaatgc tttcgatcgt aatcgggcga acacacagga
     8041 gcgcggcgtg ggtcaaccga ttgcggtggc gagcctgccg aacagcggcc cgtttggcgc
     8101 gtacgacatg atcggcaacg tctgggaatg ggtcgccgac tggtacggcg gcgactacta
     8161 tggcgtctct ccgtcggtca atcctcaagg cccggcgtcc ggattggagc gcgtcgttcg
     8221 cggtggctcg ttcaaatccg acggcgagcg cgccacgacg acaattcgac gcaaagccag
     8281 ccaggatggc tggtcggacg acatcggctt ccggtgcgtc aaagatgtct cctcgccgta
     8341 aaaatgcgcc ctttgcgcgc tgcacagtga gaagaggacg acggtattct cctctcgtgg
     8401 tatactgatc cgcgatggct atgagcgatc tcggcaagtg gctggttttg atcgggttga
     8461 gcatcgcggc gctcggcggc ctcgtgtggc tgttgggacg cttgccgttt tttggcaacc
     8521 tgccgggtga tattcggatt cagggcgaaa acctcggctg tttcgtcccg ctggtgtcga
     8581 tgctgctcct cagcctgctc ttgacggtga tattgaacgt cgtcattcga ctgttgaacc
     8641 gctgaccttg aagaccttcg agctagacta cgatattcct cccgaactca tcgcccagac
     8701 cccgatcgag ccgcgtgatg cggctcgttt gctcgtgctc gaccgtcgca gcggcgcgct
     8761 ggctcaccga cggttccgtg atgtggtcga gtatctgcgg cgcggcgacc tgctggtgtt
     8821 caacgagacg cgcgtcctca acgcgcgcct gcaggcggtg aagataccgg ccggtgggcg
     8881 cgtcgaagtg ctcctgctca aacggctgga cgcgagaacg tgggaaggtc tcgtcggcgg
     8941 gtcaggtgtg cgagtcgggt cacggttgcg gatcctgccg gcttccgagc cggcgtctaa
     9001 tggcgtcgcc gtcaaggtga tcgccgaatt cgagcgtggc gggcgcgtgc tgcgattcga
     9061 tcgcccgatt gacgatcagc tcgatatgct gggcagcgtg ccgctgccgc cgtacatccg
     9121 ccaacctctc cacgattcgg agcgatacca gaccatattt gcgcgcctgc cgggttcggt
     9181 cgccgcgccg acggcggggc ttcatttcac gcctggcttg gtgcaaacac tgcgagagca
     9241 tggcgtcgaa ttggctttcg tcgaattgcg cgtcagtctg gacacgttcc gcccgatcct
     9301 cgaagaggag gtcgagcagc atgccatcca caccgagtac tgccgcgtga gcacggagac
     9361 ggtggagcac atcgagcgtg cccggcatgc cggcgggcgc gtcatcgccg tgggtacgac
     9421 tgctgtccga acgctagaga cggctagcca ggccgatggc cgtgtggcgc cgtttgatgg
     9481 cgacacgagt ctgtttatca ctgctggcta ccggttccgc gtcatcgatg caatgatcac
     9541 gaactttcac ctgccgcgct cgacgctgct ggcgctcgtc gccgcattcg ccggtttgga
     9601 gcagctcctg gctgcgtatc gcgtcgcgat tgcggagagg tatcgcttct attcgtttgg
     9661 cgacgcaatg ctgattctct gattacagtt gtgttttcgg catgtcgtgc ggaatctcca
     9721 tgccgagtat ctcgacccag atcagattgg gggcttgtgg aggcctgtcg ccccggccac
     9781 ccgtgggtga cttgcgtcat ttgcgccaaa aaccagtgcg aatataatag tcgcatatct
     9841 cgccgcactg ctgcaatcgg gggtgtggta agcatgcagg ccgaggaagc gcgtacagtg
     9901 ctcggagccg gtcttgacga gtatcttgcg tcgctcgcac acgaaaagcg ctattcgcgg
     9961 aatacgatcg cggcctaccg caacgacttg ggacagttcg tcgatcacgt cgttgtgctg
    10021 aacgccgtgc cgggcggccg gtgggcgggc atgccgccga acgtagtcgg cgattatgtc
    10081 gcgcgactta aggaaatgac gtggcgcgat ccgtcgggcc gtgaaaagcg catggccacg
    10141 tccacggtgg cgcgcaagat cgccgcgctc aaatcgttct tccgctatct cgcgcgcatc
    10201 ggccagattg actccgatcc gacgctcggt ctggaagcgc ccaaggtcaa gaagcgtctt
    10261 ccgcgcacgc tgtcgagcgc cgaagtcgag cgcctgctcg ccgcgccggg gcagtcatcc
    10321 cagcccaagg ctttgcgcga tcgcgccctg ctcgagacgt tgtacgcgac cggcatgcgc
    10381 gtatctgagc ttgtgtcgct gaacctcgat gacgtgaacc tggtcacgca cgatgtgcgc
    10441 gtgcgcaacg atcgcggcga caaggagcgc atcgtcccga tgccggatcg ggccgtggcg
    10501 gctctgcaga tgtacctgga gcgcggccgg cctgcgctgg tcaagaacac gaaagattcg
    10561 aacgcgcttt tcttgaacca tcgcggacag aagctcaccc ggcagggcat gtggctgatc
    10621 atcaaggagt acgccgagca ggccggcatt gacttcgatg tgacacctca cacgctgcgg
    10681 cactcgttcg ccgcacatat gctggagaac aacaaggcca gcctgggcga ggtgcagcat
    10741 ttcctgggtc acgccagtgt ctccacaaca caaatctatt cgcagctcgc cgtcgagccg
    10801 aagccggatc cggttgccgg ggagtgaccg gtggccgtcc gcgcgcttcc gcttttcggc
    10861 ggctgccagc taaaggagca tcatgggata cgtcgagcaa ttactagcca agggtgaaga
    10921 gatcgtcttg cgcgcccgcc agcactgggt gacgctcatc gcttcggccc tggccaacgc
    10981 gttgttcgtg atagttgttc tggtgttgta cttcgtcgtt cgaagcatcg agttcctgaa
    11041 gccggcggaa acggtgctcc tcgttgctgc gctcatcggc gtcctgttcg gcctggcgcg
    11101 gtacagctgg gactggctgc gctggtggtc cgaagagtac atcgtcacct cgcggcgcgt
    11161 gatccagacc gaaggcatcg tcaacaagcg cacggctgac tcgtcgctgg agaaggtcaa
    11221 cgacgtgctg ctgacgcagt cattcttcgg gcgcatcctg ggctacggcg atctggaaat
    11281 tgtgacgggc agcgacgtgg gagtcaacag gctgactcgc ctgcgagacc cgatcaattt
    11341 caagaccgcc atgctggatg cgaaggcgcg gctgggagag gagtacggcg agcagatgcg
    11401 cgaggttacc gatcgtgacg tgccgaagct gatcgaggag ctcgatgcgc tgcgcaagag
    11461 gggcttgatc accgacgccg agttcaacga gaagaaagcg aggctgctgt cacaactgtg
    11521 atgatggatg atttcgtcac tcgcccacag gtcgatgaag cggtcgcggc catccgcgac
    11581 cgcattcata cccggccccg agccggggtc gtgctcggct cgggcttggg agcgctggtc
    11641 gacgccgtcg agtcgcccgt cacgatcccc tacgccgatg tgccccactg gccgcgctcg
    11701 tcggtggagg gccatgctgg gcggcttgtg tccggtaagt tggagggcgt ggatgcgttg
    11761 gtgatgcagg gacgcacgca tcactacgaa ggccattcca tggggcgggt gacgtttccc
    11821 attcgcgtga tgcagtttct aggcatcgat accttgatcg tcaccaatgc agccggtgga
    11881 ctgaacctgt cgttcagggc cggtgacgtc atgctcatcc aggatcatat caatctgatc
    11941 ggcatggctg gccaaaatcc actgcgcggg ccgaatgatt cgtcgtttgg gacgcgcttc
    12001 ccggacatgt cgcaggcgta cgactcggct ttgcgtgcgc tggcgcagcg ggtggctcgc
    12061 gagcagggca ttgagttgcg cgagggcgtg tatatctgcc tgggagggcc tagcttcgaa
    12121 acgccggcag acgtgcgctt ccttcgtctg atcggcgccg acgccgtcgg catgtcgacc
    12181 gtgcccgagg tcaccgtggc gcgccacggc ggtctgcgag tcctcggact gtcgggcatt
    12241 agtaacgtgc tgccgcccga gggcgtgacg cacacgacga cgcacgaaga agtgctcgaa
    12301 gcgggccgga cgatcgttcc taggatgctg actattgtcc ggggcgtcct gcgcgcgctc
    12361 agagcatagg gcgcgcatgg ctgcgttcgt ccgagttatc gagcagttcg ccgtcgtgct
    12421 gtataccgtg agcctcgccg gcgtcgcctg gtcgcttcga tcggcctggc tggcgatgcg
    12481 cgagagcagg gagacgctgt actcgctcga acgcgaggct gccacagcac gcatggggcg
    12541 cggcttgcta agcgcgttgg gattcgtggc gctcggcgtg accgtctttg tcgtcgctca
    12601 ggtggtcgcg ccccaattgc cggcgagcga ggcgccgccg ccgccgccgg gtcctctcgt
    12661 cacactcacg ccgacggtca ctccgtttcc cacgccgacg ccaatcagct cgccgacgcc
    12721 ggcgccttcg ccgaacgcaa ccatcctcct cgtcgaaacg attgagacat ctacgccggc
    12781 gccaacgccc gtcccgctgc ctcccgaggc atgtcccgac cccaacgtgc gcatcaccgc
    12841 gcccggcgat ggaaccgtgc tggctggtgc gtttcaggtt ttcggcacag ccaacatcga
    12901 gaactttgcg ttctacaagt tcgtgttgaa tgggccggcg acgaacttcg aagatcggac
    12961 tgccggggac gtatacgagg cgcccgtcgc cgatagttat ctcggcacac tcgacgaatc
    13021 gctggtgact atcctgctgc aatcccctgg cgcataccgc ttcaagctga tcgccgtcaa
    13081 caacgaaggc aacgaagcgc cggcatgcac catcaccctg cagttccttc cctccgtgcc
    13141 tcgctaagcg cgtctctaga aacgggagtt tggcaaatcg ctggagtgca gcccttcggc
    13201 agtccttcgc cttcgctcag gataatgcac ctgccttgcc ctccaggttg cggagacgag
    13261 atcgggatgg tagaatcgta tcgatgtcag gtctgacgat tcgtcccgcc ctgatgggcg
    13321 atatgcaaga gtgcctcgag ttcgacgcat cgtacgctac tgagtacgtc tggcaaatgg
    13381 agtcccacgc atcgagcggc gagatcagcg tcagttttcg tcacgtgcgg ctgccgcgct
    13441 caatgcgagt ggaatatccg cgggacgcgg aagccctgct ggccgagtgg cgtttgcggg
    13501 acacgctgct cgtggccgag cgcgatcggg tgaagctggg ttacgtctca ctgggtcttc
    13561 agccgggaca gcgcgcggcg cagataggcg atctggtcgt gggacgcttg tatcggcgca
    13621 ccggcgtcgg ctcggcgctg atcggcgctg cgctgcgctg ggcgcgcgat cgcggcctca
    13681 agcaggtcgt catcgaggtg cagactaaaa accatccggc gatttcattg ttgaacaagc
    13741 ttggattcac gttttgcggc tttaacgacc ggcactatct caaccgggat atcgcgcttt
    13801 tcttctctcg cagggtgcgc tagtccgcac tagagctccc cggctcgacc ccggcgattc
    13861 cgcggctttt cgacgtgaca ctcacgacga tcttcatctt catcaatacg ctcatcgcca
    13921 caggcatcgc gataaccgcc ttctcgctga tgctgtacgt ggtgcgctac aactggtcct
    13981 cgccggtggc gcgcgcgttt gtgctcatcc tcgccggcac gatgatcgta tttacgggtg
    14041 acgccgtgct gccgcgcgtg acgtcgatcg gcgcagccga gggctggctg cgcttcgagt
    14101 ggatcggcat tgcgctggcg ccggcggcct acctgcactt ctcggacgaa gtgctgcgca
    14161 cgaccaatgc cgtgtcgctg cgccggcggc gcggcgtgta cctcggctac gcctacgccg
    14221 tagtgttcgc catcgccgca ctggcaaccg attggctcgt gcagccaggc gtcacgtcgc
    14281 tggtcacgcc tcgtctttcg ccgggaccgt tcttccccgt ctatatcgcg tatttcgccg
    14341 tcgccgtcgg gttcggcttc cgcaatatcg tcgtcgcgcg cgcgcgctgc ctgacgccga
    14401 cttcgcggcg gcgaatgacc tatctcctcc tgtcgtccac cgcgccgtcg ctcggcgtgt
    14461 ttccgtttct gttggtcgtg ggtcagggcg gagccgagat cggcccgggt ttctggctgc
    14521 tcgtaatcgt ggggaacgcg acgatcggcc tgatgttgct gttgatggcc tacagcgtcg
    14581 cgtttttcgg cgtgctcgct ccggatcgcg tcgtcaagcg gcggttgatt cgattcttgc
    14641 tgcgcggccc ggtcacggct ggcgccgtcg tcacggcgat gctggtcttc acgcggctcg
    14701 agtctctact caacttgcct cgcgagacgc tgatggtagg ggctgtcgtg atctcggtcg
    14761 tcgcgttcgc cgtgctgcgc gggtcgttcc agccgctgat cgacttgctg ctgtttcagc
    14821 aggatcagga cgagctggcc tacatcgagc agcttggcga gcggctcttg acgacgaccg
    14881 atctacagca gtttctggag aatgtcctga cgtcggtcgc cgatttgctg cggacgccga
    14941 ccgcgttcat cgcgtcgcgc cgcgcgagcg atcgctggga gatgctggcg atcatcggag
    15001 cggtgcagcc ggtagagcgt gtcctgggcg agctgcaatc gggcgcccaa acgacgaagt
    15061 ctgccggcgg cccgcatcgc gccgacggtt tctggacctg gccgctgcgt tcgcgcggcg
    15121 atggcgaact gatcggcgcg ctggctgtgg caggcggtgg cgcgccgcct gagctgggca
    15181 acgatgagcg aaaacagctt gacgtgttga tgcgccaggc ggccatcgcg ctcgatgatc
    15241 gccgcttgca ggaggaagtc ttcgccgccg tcgaacagat attgccggag atcgacgtga
    15301 tccagcggca gcgcggcgcg cagcgctatc ttggctcgcc cccacagccg ccgctcgaag
    15361 ctgcgctggg cacggatccg gagttcgtca cgcacgtcaa ggatgcgctc agccacatgt
    15421 ggggcggccc caagctgacg accagccccc tgctgcgcat ggctgtcgtc gatcggatgc
    15481 tgcctagcca caatggaaat gctcccaaag cgctgcgcgc cgtcctcgtc gataccatcg
    15541 agttgctgcg gccagacggt ccgcgcagcc tgacggccgc cgagtggatg ctatacaata
    15601 ttcttgagct gcgcttcctg cgcggagagc gcgtgcgcga catcgcccga aaaatggcaa
    15661 tgagcgagag cgatctttac cgcaagcagc gcgtggcggt gggcgcggtg gcgcaggtgc
    15721 tggcgcagat ggagcagagt gcgctggaga cgacaggccc tccggccggg gagcctgtcg
    15781 ggctaactcc ggtgaacggc aatcgggggg ggagagagaa cacagcgtga gaaactcgct
    15841 gcaccagata gcgtgcgcgc gtttgacatt tgtgatgacg atcctataat aggtggcact
    15901 ttggcacatc atgcaacggg cgcttgccgg acagcggtta tcgaaccata gcggcttccg
    15961 ttttggttta cgccggagtc gagcctttag gcggttgccg gataaagcct cgactccggg
    16021 cgtgacttaa acgcaatcgg aaagcgtttt agatgtggaa aggtggcacg catggcccat
    16081 gaagcacctc aggacttatc gtgcgacgaa gcctattggg aatcgctgtt gagagacgtt
    16141 gaaacacagg ctccagggcc agccggagat cattttcgcg cgcctgcggc tgcgccacgc
    16201 gccgacgccg cgggcacggc gtggaaggcc gcgcagcgag cctcggagaa cgacgaggtg
    16261 attcaggttc agtcggtcgg ctccaatcgc ggcggcctcc tgattgagtg gaacggcctg
    16321 cgcggattct tgcccgcctc gcacctctgt gggttggccg ctcacctcga cgacgagggc
    16381 cgccgcaggg aaatcgtgca gcgcgtcggg cgtacgttca atgccaaagt gatcgagatc
    16441 gatcgcggtc agggccgctt cgtcgtgtcc gagcggttag cctgtacgca gcacgatcgg
    16501 cgcgctgctc tgctcgcgga gctcgagagc gggcaagtgc gctccggcgt catcacgagc
    16561 gtgtgctcgt tcggcgcgtt cgtcgacctg ggcggcgtgg aagggctgct gcacatcagc
    16621 gagatctcgt gggggcgtgt gaatcaccca ggcgactgtc tgaaggccgg ccagagcgtg
    16681 cgcgcgctgg tgatgagcgt cgatcgcgaa aacgagcggg tcgccctgag cgtcaagcgc
    16741 ctctatcccg acccgtggat gacggtctcg gagcggtatt cggttggtca aatcgtgcag
    16801 tgcgtcgtga ccagcgtagt ggcctttggc gcatttgcgc gcctggagga cggattggaa
    16861 gggctgattc acgtctcgga gctggccgag ggcaacttcc tgcatccgcg caatgtcgtg
    16921 tgtgaaggtc agacggtccg ggtgcgcgtg ctcaacgtcg acgccgcgca ccggcggctg
    16981 ggactgtcgc tgcgccgcgt cgacggcgac ttgctggagc gggctacgta aaccaggaag
    17041 ccgggagtat ggcacgcaag catcgagcgc gggacaaggc taagccgggt aaatctgcgc
    17101 agcacaggcc ggaaccgcgc cgccgacagt tgcgaattgc atttggtgcg cgccggcagg
    17161 aagtcgccgg cgttttgctg atcgtgttag cggcgatctc gctgctggcg ctggcgggca
    17221 tcacgaccgg ttttttgatc gactggtgga gcgtgctgct caaacgcgtc ttcggggtgg
    17281 gcgcgtatgt ggtggcagtc agctttggtc tggccggcta tttcatcatc cttcgggcac
    17341 aggggaaggt cagccggatt caatggctgc gcctgatcgg gctagaaggg gctttctttg
    17401 cggcgctggc cgcgctgcat gcggcggcgt ggtgggcgga ctcctgggcg ctcatcgatc
    17461 aggggaccgg cggcggcatc gtcggctggg gcctgtcaac cgcgctcgtc gattcggtcg
    17521 gccgtgcgcc gacggtcctg atcttcgcag ccgtcgcatt cggcgcgctg agcctggctc
    17581 tgaagattga ctggtgggcg ctcgtcgatc aaggccggaa tagactcagc acacggccgg
    17641 ttcgaccgg
//
LOCUS       DBNHXB010000429         7671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_633949, whole genome shotgun sequence.
ACCESSION   DBNHXB010000429 DBNHXB010000000
VERSION     DBNHXB010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7671
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_633949"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1431)
                     /locus_tag="AC162G_16925"
     CDS             complement(214..1431)
                     /locus_tag="AC162G_16925"
                     /inference="COORDINATES: protein motif:HMM:NF027484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HIR5077543.1"
                     /translation="MRAQPRYMILLVVSAAIAASLACTLGGGAEPPPTTPTSVSQITL
                     TPTQEPPSLTPTVVTLAAPPTATTAPPTDTPVPTATPIVGTGPGGCVLKGTYIADVTI
                     PDGTVLAPNAAFVKTWRIRNDGTCTWDAGYQLIYASGNQMSGPAAAGIPAAAPGQTVD
                     ISVNLVAPGTAGEYTGVWRLRATNNAIFGGYTVVIRVAGPPTPTATPAGGPWSGTWDT
                     SYGQMILVQTGSQVVGAYDNNFGTINGVVAGNRLSGTWAETSGARGSFDFWIEGSGLR
                     WHGNYDKSNPWCGHRPGQSDLAPCLVSTWYGAWTTKFESSVPCGDMTLNQIGDSINGN
                     CSGGGTIVGTVNGNVLQGTMTRNPGSPTQVTLPFKLFLVSGGAQFQGNYNGTSYWCGW
                     RSGSSEPAPCLKN"
     gene            complement(1523..2551)
                     /locus_tag="AC162G_16930"
     CDS             complement(1523..2551)
                     /locus_tag="AC162G_16930"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HIR5077544.1"
                     /translation="MTTVQAVQDNTDNFSPDYRVFDLRGSHFDIGRALGQSTPRFEIV
                     PWWSPSPPLDFALACAALAGDVHPPLLDEYRGFAQAQSIRYEELLQGVCRVSLRQRAV
                     GGCTSFAWRREGRILVGRNYDFRTLQRMRLRIRLAPDVALPTVGMQGSVPGGRYDGVN
                     AHGLFVSLHVVMADDPEERAPGLPFHLVPRILLETCRTVDEAVDLVTRMPHLNAFNYL
                     LADPARFLAVEAHPSRVRVVEPAGDWLVVANHYRHPDMVALQGRREATGSKQRAERAE
                     ALIEGDIELEAILRDHAGPLCEHRAHNNTLWSVVADLGARRIAYAPGMPCAVAHQAVD
                     WPSRSLPH"
     gene            complement(2862..5489)
                     /locus_tag="AC162G_16935"
     CDS             complement(2862..5489)
                     /locus_tag="AC162G_16935"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5077545.1"
                     /translation="MTSLQSGSRDKQSVIPVLDQLRADRAFMRNVAAWERIPPRAARY
                     APAPSTLHTRLLDVLRTQGIEQLYTHQAAAIESTQRGENPVVVTSTASGKTLCYNLPV
                     LNTLHREPSATALYLFPTKALAHDQISNLQSLIPALGANISARAYDGDTPRDRRKSAR
                     EAGGIIITNPDMLHTGILPHHTRWASLFESLRYVVVDELHAYRGVFGSHFANVLRRLK
                     RVGGFYGSRPQFILTSATIANPVQLAERLIEEPVTLIDDDGAPRGEKHFIFYNPPLID
                     PALGQRRSSVLEARDVAARFLLAGVQTILFARARLTTEVLLTYVRDAVAAQGGDASAI
                     HGYRGGYLAEERRAIEKALRDGSARGVVATNALELGIDIGQLDAAILAGYPGTIASTW
                     QQAGRAGRRADASVAILVASAALLDQFLVMHPRFFFERSPEHGLIAPDNLVILTNHLK
                     CASFELPFDAGEQFGRFQQTSELLDALVEEGILHREKANQRAGESVGQRYFWMADSYP
                     AQDVSLRTSSADTIVILDSSGGRAQSIGQIDRDTAPALVYEGAIYMHEGLQYLVERLD
                     WENGQAFVRPASVDYYTDASSTVDVQVLEEFERAEAGTTIKSQGEVMVTTQPVGFRKI
                     KLYTHETLGRGEIDLPEQHMQTTAYWFCVEPALADRLTIEGILFAPNDYGPNWDEQRE
                     AARKRDGYRCRQCGAPEPSPQPPPAGKTPAGEGGRQHDVHHLRPFREFAYVRGQNENY
                     KQANALENLITLCTACHRRVEAARGVRGALGGLGQVMRQIAPLYLMCDPRDIDVLAEA
                     RSPFTELPTITIFDRVAGGIGLSQECFDLHDDLLRAARDLVASCRCENGCPACVGPAG
                     EGGLDTKALTLKLLKVLAG"
     gene            complement(5486..5920)
                     /locus_tag="AC162G_16940"
     CDS             complement(5486..5920)
                     /locus_tag="AC162G_16940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HIR5077546.1"
                     /translation="MIRALVDTNVLLRMAAGGRRSRLARLWSENRFELVMSLATLTEL
                     RDVLSRPAIWRYVDRAKGMQFLALVEQRATFVQPDLSAPACRDPKDTALIAAAVGGRV
                     DFLITADPDILDDAVLVANLAGRGIRVLTSGAFLSHLAESSR"
     gene            complement(5917..6213)
                     /locus_tag="AC162G_16945"
     CDS             complement(5917..6213)
                     /locus_tag="AC162G_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077547.1"
                     /translation="MMTLQAYPVKLENGVVHSVDGSRLPERANAVLVILPEPVVDERG
                     ATIIRRPEFASLEAWQRPFDEFFTYADSHPSEADIEAVSDDELKALVHSARDAK"
     gene            complement(6416..6850)
                     /locus_tag="AC162G_16950"
     CDS             complement(6416..6850)
                     /locus_tag="AC162G_16950"
                     /inference="COORDINATES: protein motif:HMM:NF015377.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HIR5077548.1"
                     /translation="MSLAFFPQETRASCTVACLRMVLAHWGIIADEATLRACCRTTEQ
                     GTRASDAITCAQKYGLEANELRNVQWADVRELLAAEIYPIALLNLYPLNALWVMHAVV
                     VESVAEETIGYLDPIYGRKSATIVSFDQAWAMNRRRAIVVRR"
     gene            complement(6847..7191)
                     /locus_tag="AC162G_16955"
     CDS             complement(6847..7191)
                     /locus_tag="AC162G_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077549.1"
                     /translation="MIAEPIPAARVTAEAARLIGEDFVADNLGDQIGVGTPARVVSSL
                     RSAWVVPLMLTSPGYGAVGIVGVLIIDEELGHISAWTAVEEIEANVARLIEENKAELK
                     SAFERTRIRGTA"
BASE COUNT         1454 a         2409 c         2405 g         1403 t
ORIGIN      
        1 cttacggcgc gctcaacctg cacggctact tcgacgacct cggcgcggga ccgttcgcca
       61 cgcccgccga agtaccgaag ccgggttgga agccgtgatg agtgatgcgt gaagcgtaaa
      121 acgtgaaacg tgaagcatcc cttgcgcttc acgtttcatg catcacgatt cagttgtgga
      181 gcatcgtcat ggcccttcga acatcacgtc tattcagttc ttcaggcacg gcgccggctc
      241 actcgacccg ctgcgccagc cgcaccagta ggatgtcccg ttgtagttac cctggaattg
      301 cgcgccgccg gacaccagga agagcttgaa cggcagggtc acctgcgtcg gagagccggg
      361 attgcgcgtc atcgtgccct gcagcacgtt cccgttcacc gtcccgacga tcgtcccgcc
      421 gccagagcag ttgccgttga tactgtcgcc gatctggttg agcgtcatgt caccgcaggg
      481 aacagagctt tcgaatttcg tcgtccacgc gccgtaccag gtggacacga ggcagggcgc
      541 gagatcggat tgaccggggc gatggccgca ccacgggttt gacttgtcgt agttgccgtg
      601 ccaccgcagg ccgctgccct cgatccagaa atcgaacgag ccgcgggcgc cgctcgtctc
      661 cgcccacgtg ccgctcaggc gattgcccgc gaccacgccg ttgatggtgc cgaagttatt
      721 gtcgtacgcg ccgactacct ggctgcctgt ctgcacgagg atcatctggc cgtaacttgt
      781 atcccacgtt ccgctccagg gaccgccggc cggcgtggcc gtcggcgtcg gcgggccggc
      841 gaccctgatg acgacggtgt agccgccgaa gatggcgtta ttggtcgcgc gcagccgcca
      901 gacgccggtg tattcgcccg ccgtcccagg cgcgaccagg ttgacagaga tgtcgaccgt
      961 ctggccgggc gctgccgccg ggatgccggc cgccgccggg ccgctcatct gatttcccga
     1021 ggcgtagatg agctgataac cggcatccca cgtgcacgtg ccgtcgttgc gaatacgcca
     1081 cgttttgaca aacgctgcat tcggcgcaag caccgtgccg tcaggaatcg tgacgtcggc
     1141 gatgtacgtt cccttcagca cgcagccgcc ggggccggtt ccgacgatgg gcgtagctgt
     1201 gggcacaggc gtgtcagtcg gcggggcggt ggtcgccgtc ggaggcgcgg ccagcgtcac
     1261 gacggttgga gtcaggctcg gcggttcctg cgtcggcgta agcgtgatct gcgacacgct
     1321 cgtcggcgtc gtcggcggtg gctcggcgcc gccgcccaac gtgcaggcca ggctggccgc
     1381 gatggcagcg gagacgacga gcaagatcat gtaacgaggt tgagcacgca tagacatgtc
     1441 cccccatgcg atcatcaagt cttagttaag acagcgcgat ccagtaccag aggacgatta
     1501 tagtcacgct gcgcgatttc tcctaatgcg gcaggctgcg cgacggccag tccactgcct
     1561 gatgcgccac cgcgcagggc atgcccggcg cataggcgat gcgccgcgcg ccgagatcgg
     1621 cgacgacgga ccacagcgtg ttattgtgcg cacgatgctc gcacagcggc ccagcgtgat
     1681 cgcgcaggat cgcttcgagc tcgatatccc cctcgatcaa tgcctcggct cgctccgccc
     1741 gttgtttcga cccggtcgcc tcacgccggc cctgcagcgc aaccatatcc ggatggcgat
     1801 agtggtttgc cacgaccagc caatcgcccg ccggctctac gacgcgcacg cgtgaagggt
     1861 gcgcttcgac ggcgaggaag cgagccggat cggccagaag ataattgaag gcgttcagat
     1921 gcggcatgcg tgtgacgaga tcgaccgcct cgtcgacggt gcggcatgtc tctagcaaga
     1981 tgcgcggaac caggtgaaag ggaaggcccg gcgcacgctc ttcgggatcg tcggccatca
     2041 cgacgtgcag cgagacgaac agcccgtgcg cgttcacgcc gtcgtagcga ccgcccggca
     2101 cgctgccctg catgccgacg gttggaagcg ccacatccgg cgcgaggcga atgcgcagcc
     2161 gcatgcgctg cagcgtgcga aagtcgtaat tgcggccgac gagtatgcgc ccctctcgcc
     2221 gccaggcgaa cgacgtgcat ccgccgacgg cgcgctgccg cagcgaaacc cggcagacgc
     2281 cctgcaaaag ctcctcatat cggatggact gggcctgggc gaagccgcga tattcgtcga
     2341 gcaggggcgg atggacgtcg ccagccagcg cggcgcacgc cagcgcaaaa tcgagcggcg
     2401 gcgacgggga ccaccagggc acgatctcga aacgaggcgt cgactgcccc agtgcgcggc
     2461 cgatgtcgaa gtgcgacccg cgcaagtcaa atacccgata atcgggagaa aaattgtctg
     2521 tattgtcctg cacagcctgg actgttgtca tatgcgcagc atgccgtttg tcactatggt
     2581 gcaaacgatg gcgggtatat cctgtatcta gacttggaca taagcaatta ccttatcgcc
     2641 caccccaggt agttgcagag tttgagtccg ttctctgtgc ggtcccctgg ctgccagaga
     2701 aacagattct cctcctccct tggctgccgt cccggccgtg agcccccgcc acgaccggga
     2761 ctggccttta tataaccggg ttccttacga tccgacacct gatacccgac ggaggagaga
     2821 ttcctcactg cgttcggaat gacactagcc gacgtaggga actatcccgc cagaaccttc
     2881 agcagcttga gcgtgagggc tttcgtatct aatcctccct cgcctgccgg gccgacgcac
     2941 gccgggcagc cgttctcgca gcggcacgag gcgaccaggt cacgcgccgc gcgcagcagg
     3001 tcgtcgtgca gatcgaaaca ttcctggctc aggccgatgc cgcccgccac gcggtcgaaa
     3061 atcgtgatcg tcggcagctc tgtaaatggc gaccgagcct cggccagcac gtcgatgtcg
     3121 cgcgggtcgc acatcaagta tagcggcgcg atctggcgca tcacctgccc caacccgccc
     3181 aacgcaccgc gcacaccgcg cgccgcttcg acgcgccggt gacaggccgt gcacaacgtg
     3241 atcaggttct ccagcgcgtt cgcctgcttg tagttctcgt tctggccgcg cacgtaagcg
     3301 aactcgcgga acgggcgaag gtgatgcacg tcgtgctggc gccctccctc tcctgccggc
     3361 gtttttccgg cgggaggggg ctggggtgag ggctccggcg ctccgcactg ccggcagcga
     3421 taaccgtcgc gcttgcgagc ggcctcgcgc tgctcgtccc aattcgggcc gtaatcgttc
     3481 ggcgcgaaga ggatgccctc tatcgtcaag cgatcggcga gcgccggctc gacgcaaaac
     3541 cagtacgccg tcgtctgcat gtgctgctcg ggcaggtcga tctccccccg gccgagcgtc
     3601 tcgtgtgtgt acagcttgat cttgcggaag ccgacgggct gtgtggtgac catcacttcg
     3661 ccctggctct tgatcgtcgt tccggcctcg gcccgctcga attcctccaa cacctgcacg
     3721 tcgaccgtgc tgctcgcgtc ggtgtagtaa tcgacgctcg ccggtcgcac gaacgcctgg
     3781 ccgttctccc agtcgagccg ctcgaccaga tactgcagac cctcgtgcat gtaaatcgcg
     3841 ccttcgtaaa ccagcgcggg agcggtgtcc cgatcgatct gcccgatcga ctgcgcccgg
     3901 cccccactcg aatcgagaat cacgatggtg tcggcgctgc tcgttcgcag cgagacatcc
     3961 tgcgccgggt aagaatctgc catccagaaa tagcgctgac ccaccgattc gccggctcgc
     4021 tgattcgctt tctctctgtg cagaatcccc tcctcaacca gcgcgtctaa cagctcgctc
     4081 gtttgctgga agcgtccaaa ctgctcgcct gcgtcgaacg gcagctcgaa cgaagcgcac
     4141 ttgaggtgat tggtcaggat gacgagatta tccggagcga tcaggccgtg ctcggggctg
     4201 cgctcgaaga agaagcgtgg atgcatcacg aggaactggt cgagcagcgc ggcgctggcg
     4261 acgaggatgg cgacggatgc gtcggcgcgc cggcccgccc ggcctgcctg ctgccaggtg
     4321 gatgcgatcg tgccgggata gccggccagg atggctgcgt cgagctggcc gatgtcgatg
     4381 cccagctcga gcgcgtttgt cgcgacgacg ccgcgcgccg acccatcgcg cagcgccttc
     4441 tcgatcgcgc ggcgctcttc ggccaggtag ccgccgcgat agccgtgaat cgcgctcgca
     4501 tcgccgccct gcgccgccac cgcgtcgcgc acgtaggtga gcaggacttc ggtggtgagc
     4561 cgcgcacggg cgaagaggat cgtctgcacg ccggccagca agaagcgcgc cgcgacgtcg
     4621 cgcgcttcga gcacggacga gcggcgctgg cccagcgcgg ggtcgatcag cggcgggttg
     4681 tagaagatga agtgcttctc gccgcgcggc gcgccgtcgt cgtcgatcag cgtgaccggc
     4741 tcctcgatga gtcgctcggc gagttgaacc gggttggcga tcgtcgccga ggtgaggatg
     4801 aattgcgggc gggagccgta gaaaccgcct acccgcttca ggcggcgcag gacgttggcg
     4861 aaatggctgc cgaacacgcc gcgatacgcg tgcagctcgt cgacgacgac gtagcgtagc
     4921 gactcgaaca gcgacgccca gcgcgtatgg tgcggcagga tgcccgtgtg cagcatgtcc
     4981 gggttggtga tgataatccc acccgcttcg cgcgccgatt tgcggcggtc acgcggcgtg
     5041 tcgccgtcgt acgcgcgggc ggagatgttt gcgccaagag caggaattag agattggaga
     5101 ttggagattt ggtcgtgcgc cagggccttg gtgggaaaga ggtacagcgc cgtcgccgat
     5161 ggctcgcggt gcagtgtgtt gagcacgggc aggttgtaac acagcgtctt gccggaggca
     5221 gtcgacgtga cgacgacggg gttctcgccg cgttgagtcg attcgatcgc cgccgcctga
     5281 tgcgtgtaga gttgttcgat accttgtgtg cgtaatacgt cgaggagtcg agtgtggagc
     5341 gtggagggtg ccggcgcgta gcgggccgcg cgcggcggga tgcgctccca ggcggcgacg
     5401 ttgcgcatga aggcgcggtc ggcgcggagt tgatcgagga ccggaatgac cgattgtttg
     5461 tcgcgagatc cggattgcag cgatgtcaac gagatgattc tgcgagatgt gaaaggaatg
     5521 cgccggatgt gaggacacga atcccacggc cagcaaggtt cgcgaccaac acagcatcat
     5581 ccagaatgtc cgggtcagct gtgatcaaga agtcaacgcg tccaccgact gcagctgcaa
     5641 taagggctgt atctttcggg tcacggcacg ctggggcaga caggtcgggc tggacgaaag
     5701 tggcacgctg ctcaaccaag gcgaggaatt gcatgccttt agcccggtcg acgtaccgcc
     5761 atatcgccgg gcgggagaga acgtcgcgca gttcggtcag tgttgccaat gacatcacaa
     5821 gttcgaaacg gttctcgctc cacagtcgtg caaggcgtga gcggcgtcca ccggctgcca
     5881 tccgtagcaa aacgttcgtg tcaacgagcg cacgaatcac ttcgcgtccc gcgctgaatg
     5941 aacgagtgct ttcaactcgt catcgctcac ggcctcgatg tccgcttccg acggatggga
     6001 gtcggcgtag gtgaagaact cgtcaaatgg gcgctgccac gcctcaagcg aagcaaactc
     6061 aggccggcga ataattgtag cgccacgttc atcgaccacc ggctcgggca aaatgaccag
     6121 aacagcgttc gctcgttcag gcagccgcga gccatccact gaatggacta cgccattttc
     6181 gagttttaca ggataagcct gcagcgtcat cacgaaatac ctccatgtta ggtacggtga
     6241 atctaaagta attatagcac gcgcaccggg ctgcgctccc acccggtgat attgcgccgg
     6301 aaggcgcatc ggcgcggagt tgatcgagga cggattggat agacacccgg ttaagtagct
     6361 tgtagagtgg agatacttgg agcagttacg ctgattacag tcgtaaaaat gatatttagc
     6421 ggcgtaccac aatcgcacgc cgccgattca tagcccacgc ctgatcaaat gaaacgatag
     6481 tggccgactt acggccgtaa atggggtcaa gatatccgat cgtctcctca gcgacggact
     6541 ccacgacaac tgcgtgcatt acccatagcg cattgagtgg gtacagattg agcagcgcga
     6601 tgggatagat ttctgcagcc aacagttcac gcacgtcagc ccactggaca ttccggagct
     6661 cattggcctc tagcccatat ttctgcgcgc acgtgatggc atcgctcgcc cgtgtgccct
     6721 gctccgttgt ccggcagcac gcgcggagag tggcctcatc tgcgataatt ccccaatgcg
     6781 ccagcaccat tcgcaaacag gccacagtgc acgaggcgcg cgtttcctgg gggaagaaag
     6841 ccaaactcac gccgtgcccc tgatgcgggt tcgttcaaat gcggatttca gctcagcctt
     6901 attttcttca atgagccgcg ctacgttcgc ttcaatttcc tccaccgctg tccacgcact
     6961 gatgtgacca agctcctcat cgataatcag cacgccaact atgcccactg caccatagcc
     7021 cggcgaggtg agcatcagcg ggacaaccca agccgagcga agggacgata ccactcgcgc
     7081 tggcgttccc acaccgatct gatcacccag attgtctgct acaaaatcct caccgatgag
     7141 gcgcgcagcc tcggccgtaa cccgagctgc cgggatgggt tcagctatca tcgcatgact
     7201 ccacgccatt ttcaagtttg accggacaag ctcgcatcat cgtatcacct cgcatcacac
     7261 aatctgtagt ccggttctca aaaccattat agcacacatg cccgaataac cttgcgaagg
     7321 ctatgtcgtg cgaggccctt cgctctatcg cacgacgact ggtggcgctg atcgtgcgac
     7381 gctccctacg attatccact tgacaaagcg cacgtcatag aaaatgatca tagcgaaggg
     7441 gaaggggagg aatctctgcg ccaatcagca acgccaccgg gcaggggtag agattcctcg
     7501 tcgcaaacgg cgctcctcgg aatgacactg accggttact gatcaccgaa ctacactcgc
     7561 tatgaagact ggctaccgaa agacgctgcg tgacgtgtgg cgcgacaagg ggcgcacgat
     7621 cctcgtcgtg ctgagcatcg gcgtcggcgt gttctccgta ggcctggtgt c
//
LOCUS       DBNHXB010000430        13243 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1641008, whole genome shotgun sequence.
ACCESSION   DBNHXB010000430 DBNHXB010000000
VERSION     DBNHXB010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 13243)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13243)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13243
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1641008"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /locus_tag="AC162G_16960"
     CDS             complement(<1..1447)
                     /locus_tag="AC162G_16960"
                     /inference="COORDINATES: protein motif:HMM:NF024777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HIR5077550.1"
                     /translation="MKTSTAKRAPKPPAGPQGLQPGAPMWNHGEILFTNEVAAKIAAS
                     GAGWVRLNFRLGPNFANWTDTSAFGYSALSRYDTIIDNALNNNLKVLGLLSNEAWNTR
                     NPPPAEWQENNAEEERLVGYWKFDEGSGATASDASGTGNDAVLIDSPVWSPDISSTIV
                     YENTYSLLFDAADGYVAVPHHSSLQLAADVTVAAWVKTDNADGSADVIVSKWGSAGNR
                     NYWLGKLNGTSLAFYVDNAQNVTADLSLINDGLWHHVAGVADAAKGSLTIYVDGVARN
                     TATYDGTSETGTADIHVGASADSPIQIWAGGIDDVRVYARALSGTEVEALASGTVERA
                     NGDNRYIQEFEAGAARVLIPHFAGKIDHWQVWNEPNASLTWVYPSNFAWLLRRVYVAA
                     KESGVPNLTIVSGGLLSTHSQADTSITTSNTGADYLRDTYNRGRINAGWETIKSTYGS
                     YPLDVIGQHLYIDAWGRTTARRIERAVRLVHD"
     gene            complement(1646..4270)
                     /locus_tag="AC162G_16965"
     CDS             complement(1646..4270)
                     /locus_tag="AC162G_16965"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBM96 family carbohydrate-binding protein"
                     /protein_id="HIR5077551.1"
                     /translation="MHILRSKASTKSTGAVRRLVVVFLAVPPLLISSLVACSPIGPTS
                     APAATSSPIRSTSPSESSPIGSTSVPAATRRVPQDYPTIQAAIDAAQNGDVVLVAPGV
                     YGESLVLSGKTITLASQFYTTGDTQVIDSTIIDGQAGPAVVTVDASVGPDTQIVGFTI
                     RNAEDGIKARGKLHILNNRITGTRDGIDFQNAGGLVRDNTLENNRDDGIDSDGATGVT
                     IENNIIRSNGDDGIEIRLHEYSGVTLTLSLRNNVITGNGEDGIQLIDYPDISNRVFDI
                     RYNLIADNAQAGLGLMDNGESNEDVRGASIPERIHLFNNTLSGNPYAVTGGDNLIALN
                     NLFVNSAIAGLKNVDAGSISAANLFWNNGTNDQGSNIDLSTTLFSDPLLDAAYHLLPG
                     SPAMDAGMAHFEWNGEAVLDLPPGAYSGAAPDLGAYEFDFTAPMPAPTPGPVLTFAPA
                     DDASITAQSPNAKHSSSTTLEVDNSPVRNFLLKFAVTGVNGRAVSSAKLRLYSVGDSD
                     TGGDFHRVADNTWTEQTVTWNNAPAGDEAPIASLGAVAVNTWYEVDLTSLIAGDGIYS
                     LRVTSPSANGADYSSKEGTNAPQLVISVQDFPASTPASTETATPPGLIRFAVIGDYGE
                     AGQPEQDVANLVKGWNLDFVITTGDNNYPDGAAETIDQNIGQYYHELIYPYTGSYGPG
                     ATTNRFFPSLGNHDWHTPNAQPYLDYFTLPGNERYYDFVWGPVHFFAVDSDGQERDGN
                     TSSSTQAAWLQNRLAASTAPWQLVYMHHPPYSSGAVHGSTSNLQWPYQAWGADAVLAG
                     HDHTYERILQDGIPYFVNGLGGRSIYAFGTPVSGSQARYNDDYGAMLVEASESQIVFQ
                     FIARTGAVIDTYTLGASP"
     gene            complement(4292..5326)
                     /locus_tag="AC162G_16970"
     CDS             complement(4292..5326)
                     /locus_tag="AC162G_16970"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015163222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5077552.1"
                     /translation="MSLWVLYAAITAGTIPGTAAIRYPGRAPDVNAVQQIAATPDPLS
                     HHAFLPIIFRPYDMLVGAGDIANCSHTRDESTAQLLDGMAGTVVTLGDNAYPDGTPAQ
                     FNNCYGPTWGRHKARTRPAPGNHDYHTPGAAGYYAYFGAAAGDPAKGYYSYNVGAWHI
                     IALNSEVDHSAGSAQEQWLRADLAANQSVCTLAYWHKPRFSSGMHGSNSGVQPFWQAL
                     YDYGADVVLNGHDHTYERFAPQNPSGQADPSRGIREFVVGTGGASLYSFPTIRPNSEV
                     RNNTTWGVLKLTLHPTRYDWQFVPIAGQTFTDAGSANCVRAGLAPTDMPTSSATPTRT
                     PSPVAATSFT"
     gene            complement(5450..10549)
                     /locus_tag="AC162G_16975"
     CDS             complement(5450..10549)
                     /locus_tag="AC162G_16975"
                     /inference="COORDINATES: protein motif:HMM:NF027442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HIR5077553.1"
                     /translation="MRKFFFLTAGILTAIAALAVYQSVRAATLIVPDNYPTIQAAIDA
                     ANPGDTILVRPGTYLENLTLNKSVILTAEVFDATDPTRNTTIVDGGPSSLVATIAIPA
                     GLSPMPTIRGFVIRNGSDGIVIRSESIVEYNYLLQSVGDLNDYGQDSGGINRYNVFFH
                     SGDDSIDLDNMNRPLVIENNRMMYSVDDGIEIRLQDLSAPAQPIEITIRNNEIIGSNE
                     DGIQLIDDSQPLDSNRRFVIRGNLIANCRKAGIGLIPNQASTEDYSGANIVEAIRVYN
                     NTFYGNDYGISGGDNLVAFNNMIANSVTKGAWRVQGLAGANSVVAYTLFHNNSLDTDQ
                     SDLGAGNLFGQNPLFVSPPNAGPDNVWGTVDDDFSGLILQPGSPAIDAGVTQYIANDG
                     EAVPPAPITNFSGLAPDLGWKEFDPNQPLTPTAIPTATSTPGLPDLIFADGFESGDLS
                     AWTSNSNDLGDLSVSAVAALMDSQGLQAVIDDSNTIYVTDDRPTAEPRYRARFYFDPN
                     SIPMASGDAHYIFKGFMGTSTEVLRVEFRQSSGIYQLRAALLLDDSVTWVSANWFTLS
                     DAPHFIELDWRAATAMSANDGGLTLWIDGTQQANLTGRDNDTWRIDRARLGALNGIDA
                     GTRGTYTFDAFESRRFTYIGPATVSPTLTPAPTSTPTPTPRPTLTPVPAGVIRLAVIG
                     DYGSDSQAEQDVADLVKGWNPDFVITTGDNNYPDGAAETIDRNIGQYYHEFIYPYTGS
                     YGAGATTNRFFPSLGNHDWNTPNVQPYLDYFTLPGNERYYDFTWGPVHFFAVDSDGRE
                     PDGNSSASTQGIWLQTQLAASTSPWNLVYMHHPPYSSGATHGSTTVMQWPYQAWGADA
                     VLAGHDHTYERILPDGIPYFVNGLGGASIYTFSTPVSGSQVRYNGDYGAMLVEASETQ
                     IDFQFIARTGAVIDTYTLDASPTATPTETATPTETPTPSHTPTPIATNQTHIQYTPST
                     EDFANPERGFMKQSSIWIDQPLDPTKIRAVNPSDSLVWIYFRLDNYRDRPIDQNGLST
                     VSSAFQTARDKGLKLVIRFIYNDGPGSTNDPDLANPDAPIDLVLQHIDQLRPILIENA
                     DVIAVMQAGFVGHWGEWHSSKYLHPLEYRRAIVDALLQLLPTQRMLMLRYPRYKEIFF
                     QGPLTESQAFSQTDASRIGHHNDCFLSGDDDEGTYQSQTLQQPQHESLYCAGQDQIAC
                     WKGFVAQEGRFTPVGGETCAVNPPRTECPNAQLELEMLHWSFINNDYRQEVLDGWVTG
                     GCMDTIRRRLGYRLVLKEAVVPQTIQPGGALNMDVVLRNDGYASLYNPRPVYVVLHNA
                     NNRYDIPLPSIDPRRWEAGRDHTITVSAPVPTNIVSGTYALSLWLPDQFTSLRNTPAY
                     AVRLANTNVWDTATGLNILTSNLTVLAGAPTPTDTLTPSATPTPSHTPTSTPTPTPTP
                     TDTPTPSATPTPSRTPTSTDTPTPTSTPTGTSTPTDTPTPTAMPTRTPTATATPSDLI
                     FADGFESGSLSAWSSSTTDVGDLSVSAAAALIGGKGLKAVIDDNNSIYVADDRPSAEP
                     RYRARFYFDPNSISMIDNDNHYLFYGYTGASTLVWRVQFRYSNGSYQLRVALRNDGST
                     WKTSSWFSISDAPHFVEIDWRAATAGGANNGGLTLWIDGAQRANLIGVDNDTRRIDRV
                     RLGAVSGIDSGTRGVYYFDAFASRRQTYIGP"
     gene            complement(10812..11942)
                     /locus_tag="AC162G_16980"
     CDS             complement(10812..11942)
                     /locus_tag="AC162G_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5077554.1"
                     /translation="MSISQELYNRLAETIRPLVSVSNSKQITNWLWIVVGLVLGNSPA
                     LSKIATYLPMDTQAESRVTLIRRWLKNLKVDVWAFYEPLLAHVLHGWRAVDVFVILDG
                     VMVFGDRWQVFRVSLQHGCRAIPLMWMVVDGTGVIAVERLAERLGRVAQFLRRRVHRV
                     TFLADRGFRDCDWAKLARKLGWHYDLRITCNTYITLEDGRACRVDELGVPRGQRRYFQ
                     NVCVTQEGLWRANLSVGWTEGDAKTEPELLAVISDQSACRARLREYGRRMSIEQSFRD
                     DKSGGFDLEHTRLQHADRLERLLLAMAVATLWCHELGERVLAHGESARRQIDPGPTRE
                     LSLFQLGLRWLKRCVSVAIHDLPIFRARLSNLKLAPVVGVPV"
     gene            complement(12090..12536)
                     /locus_tag="AC162G_16985"
     CDS             complement(12090..12536)
                     /locus_tag="AC162G_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015202135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR5077555.1"
                     /translation="MKIADHLRDVARLFLDTAPLVYYVEEQARYLALVEPVFERLDRG
                     DFMAVTSPVTLAECLVVPYRDGRLDIGREFAALILNGANTSFVVIDRDVADRAAELRA
                     RYNLNLPDAFQVSVALAAGCDAFLTNDTALKRIIELRVIVLDEVDT"
     gene            complement(12533..12787)
                     /locus_tag="AC162G_16990"
     CDS             complement(12533..12787)
                     /locus_tag="AC162G_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077556.1"
                     /translation="MSADLLNDMIKQVDKLTPDEQLRLATYLVDKARTAYPVAEPRRK
                     WAEIRGIARPSLFGEDAQAWVSRTRREGDERREQQLRRQS"
     gene            complement(12836..13006)
                     /locus_tag="AC162G_16995"
     CDS             complement(12836..13006)
                     /locus_tag="AC162G_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit M"
                     /protein_id="HIR5077557.1"
                     /translation="MLTPGRYFGEEEVEDDDEPFDDKIKRLTAKREEQFVESARLEQA
                     IRDNLSRLGHGV"
BASE COUNT         2527 a         3822 c         4106 g         2788 t
ORIGIN      
        1 tatcgtggac cagcctcaca gccctctcga tccgcctggc cgtcgttctc ccccacgcgt
       61 cgatgtacag gtgctggccg atcacgtcga gtggatagga gccgtaggtg ctcttgatcg
      121 tctcccagcc ggcattgatg cgaccccggt tatacgtatc gcgcagatag tcggcgccgg
      181 tgttgctcgt cgtgatgctc gtgtccgcct ggctgtgcgt gctcagcaag ccgcctgaga
      241 caatcgtcag gttcggcacg ccgctttcct tcgccgcgac gtagacgcgc cgcagcagcc
      301 aggcgaagtt cgagggatag acccaggtga ggctcgcgtt cggctcgttc cacacctgcc
      361 agtgatcgat cttgccggcg aagtgaggaa ttaacacgcg ggcggcaccg gcctcgaact
      421 cctggatgta tcgattgtcg ccgttggcgc gctccactgt gccgctagcc aaggcttcga
      481 cttctgtacc ggagagggcg cgggcgtaga cgcgcacgtc gtcaatccca ccggcccaga
      541 tttgaatggg gctgtctgcg cttgcgccga catgaatatc tgccgtgccg gtctcagacg
      601 ttccgtcata ggtggccgta ttgcgcgcta ccccatcaac atagattgtc agcgaccctt
      661 tcgcggcatc agcgaccccg gcgacgtggt gccataggcc atcgttgatg aggctcaagt
      721 cggcggtgac attctgcgca ttatccacgt agaaggcaag gctggtacca ttcagcttgc
      781 caagccagta atttcgattg ccggcagacc cccacttcga cacaatcacg tcggctgagc
      841 cgtccgcatt atcagtcttg acccacgccg ccaccgtcac atccgcagcc agttggagac
      901 tgctgtgatg cggaacggcg acatagccat cggcggcatc aaacagcagc gagtaagtgt
      961 tttcatagac gatggtggag ctgatgtccg gactccagac ggggctgtca atgaggacag
     1021 cgtcattgcc agtgcccgag gcatctgagg ccgtggcacc gctaccttcg tcgaacttcc
     1081 agtaacccac gaggcgctcc tcttcggcat tattctcttg ccactcagcg ggcgggggat
     1141 ttctcgtatt ccaggcttca ttcgagagca gacccaacac tttcaggttg ttgttcagcg
     1201 cgttgtcgat gatcgtgtcg taacgcgaaa gcgcggagta cccgaaagcg ctcgtgtctg
     1261 tccagttcgc gaagttcgga cccaggcgga agttgagccg gacccagccg gcgccggacg
     1321 cggcgatctt cgcggcgacc tcgttcgtga agaggatttc gccgtgattc cacatcggcg
     1381 cgccgggctg gaggccctgc gggccggcag gcggttttgg cgcgcgtttg gcggtgctgg
     1441 ttttcatggc tatttcctca tttctcacgc agcagttccc ctggtatttc cggcccccgt
     1501 ttgagcagtt gttccagcgt cacgcctttc tgccgcagtt cttgcccgat cgcatccagg
     1561 acggtgttca caaccacttc tgccggttgg atgaggacgc cagcctcggt ctcctattac
     1621 acatcagcgg cactcagaac agccgtcagg gcgatgcgcc aagcgtgtac gtgtcgatca
     1681 ccgcgcccgt gcgcgcgatg aactgaaaga cgatctgcga ttcactggcc tcgacgagca
     1741 tcgcgccgta gtcgtcgttg tagcgcgcct ggctgccgga caccggcgtt ccgaacgcgt
     1801 agatggatct gccgcccagg ccattgacga agtatggaat gccatcctgc agaatgcgct
     1861 cgtacgtgtg atcgtgtccg gccagcaccg cgtccgcgcc ccacgcctga tacggccact
     1921 gcaggtttga cgtagagccg tgaaccgccc ccgaagagta aggcgggtga tgcatgtaga
     1981 cgagttgcca gggcgcggtc gaagcggcaa gccggttttg cagccaggct gcttgtgtgg
     2041 acgagcttgt gtttccgtcc cgttcttgac cgtcactgtc gacggcgaag aaatgcaccg
     2101 gcccccagac gaaatcgtag taacgctcgt ttccgggcag ggtgaagtaa tccaggtaag
     2161 gctgggcatt gggcgtatgc cagtcgtggt tgcccagcga cgggaagaaa cgattggtcg
     2221 tcgcaccggg gccataagag ccggtgtagg ggtagatgag ctcgtgataa tactgcccga
     2281 tgttctgatc gatcgtctcg gctgcgccgt ccggatagtt gttgtcgccg gtggtgatga
     2341 caaagtcgag gttccagccc ttgaccaggt tcgcgacatc ttgttcaggc tgcccggctt
     2401 caccgtagtc gccgatgacc gcgaagcgga tcaagccggg cggcgtcgcc gtttcggtcg
     2461 atgccggtgt cgacgccggg aaatcctgca cgctgatgac gagttgaggc gcgtttgttc
     2521 cctctttgga agagtaatct gctccattgg cggaaggcga agtgacgcgc aggctgtaaa
     2581 tgccgtcgcc ggcgatcagc gaggtcaggt cgacttcgta ccaggtattg accgccaccg
     2641 cgcccagcga agcgatcggc gcctcgtcgc cagccggggc gttgttccag gtgacggtct
     2701 gctccgtcca cgtgttgtcg gcgacgcggt gaaaatcgcc gcctgtatcg gagtcgccga
     2761 cgctgtacag gcgcagcttc gcgctggaca cggcacggcc attgacgccg gtcacagcaa
     2821 acttgagcag gaagttcctg accgggctgt tgtccacctc gagcgtcgtc gacgagctgt
     2881 gtttggcgtt gggtgattgg gccgtaatcg aagcatcgtc ggccggcgcg aacgtcaaga
     2941 ctgggccggg cgtgggcgcc ggcatcggag ccgtgaagtc gaactcgtac gcgcccaggt
     3001 caggcgctgc gcccgaataa gcgcccggcg gcaggtccag gaccgcttcg ccgttccatt
     3061 caaagtgggc catgccggca tccatcgccg gactgccggg caggagatga taggcggcat
     3121 ccagcagcgg atcgctgaac agcgtcgtgc tgaggtcgat gttgctgcct tgatcgtttg
     3181 tgccgttgtt ccagaacagg ttggcggcgg agatcgatcc ggcgtcgacg tttttcaggc
     3241 cggcgatcgc ggagttcacg aacaggttgt tgagcgcgat caggttgtcg ccgccggtca
     3301 cggcgtaggg attgccggag agggtgttgt tgaacaggtg aatccgttcg ggaatgctgg
     3361 ccccccggac gtcttcgttc gactctccat tgtccatcag gccaagcccg gcctgggcgt
     3421 tatcggcgat cagattgtat cggatgtcga agacgcgatt ggagatatcc ggatagtcaa
     3481 tcaattggat gccgtcttcg ccgttgccgg tgatgacgtt gttgcgaagc gagagtgtga
     3541 gcgtcactcc actgtattca tgaagtcgaa tctcgatgcc gtcgtcgccg ttgctgcgaa
     3601 tgatgttgtt ctcgatcgtg acgcccgtcg cgccgtcgct gtcgatgccg tcgtcgcggt
     3661 tgttttcaag cgtgttgtcg cggacgaggc cgccggcgtt ttggaagtcg atgccatctc
     3721 gcgtgccggt gatgcggttg ttgagaatgt gcagttttcc acgagccttg atgccatcct
     3781 cggcgttgcg aatggtgaag ccgacgatct gcgtatccgg cccaaccgac gcgtcgacgg
     3841 tgacgacggc cggaccagct tgaccgtcga tgatcgtgct gtcgatgacc tgagtgtcgc
     3901 cggtcgtgta gaactgcgag gcgagcgtga tcgttttgcc ggacagaacg agcgactcgc
     3961 cgtacacgcc gggcgcgacg aggacgacgt cgccgttttg ggcggcgtcg atcgcggcct
     4021 gaatcgtcgg gtaatcttgg ggaacgcgcc gggtagcagc cggcacagac gttgagccga
     4081 tgggcgaact ttccgatggg gaagtgcttc gaattggcga cgaggtggca gccggcgcag
     4141 acgttgggcc gatcggcgaa caggcgacga gcgaactgat gaggagcggt ggaacggcaa
     4201 ggaaaacgac gacgagccgg cgcaccgccc ctgtgctttt ggtgctcgcc ttggagcgaa
     4261 ggatgtgcat cgccgccacc cgtcctagaa ctcacgtaaa tgatgtcgcc gccaccgggc
     4321 tgggggtccg cgtcggagtc gcgctcgatg ttggcatgtc ggtcggggcc agaccggcgc
     4381 ggacgcagtt ggcgctgccc gcatcggtga aggtctgccc ggcgatcggg acgaattgcc
     4441 aatcgtagcg ggtcggatgg agggttaact tcagcacacc ccaggtggtg ttgttgcgca
     4501 cctcgctgtt ggggcggatg gtggggaagg aatataagct cgctccgccc gtgcccacga
     4561 caaactcgcg gatgccccgg ctcggatcgg cttgtccgct cgggttctgc ggcgcaaacc
     4621 gctcgtaggt atggtcgtgg ccgttcagca ccacgtcggc tccataatcg tagagggcct
     4681 gccaaaacgg ttgaacaccg gagttgctac catgcatgcc cgagctaaag cgcggcttgt
     4741 gccaatacgc cagcgtacac acgctctgat tcgcggccag gtcggcccgc agccactgct
     4801 cctgggccga gccggcgctg tggtcgacct cgctgttgag ggcgatgatg tgccatgccc
     4861 ccacgttgta ggagtagtag cccttcgccg ggtcgcccgc cgccgcacca aagtaggcgt
     4921 agtagccggc cgcaccgggg gtatggtaat cgtggttacc cggcgcgggg cgggtgcgag
     4981 ccttgtggcg gccccaggtt ggcccgtagc agttgttgaa ctgggccggc gtaccgtccg
     5041 ggtaggcgtt gtcgcccagg gtgacaacgg tgccagccat gccgtcgaga agttgggcgg
     5101 tcgactcatc ccgggtgtga ctacaattgg cgatgtcgcc tgctccgacc agcatgtcgt
     5161 agggcctaaa gatgatcggc aggaaggcgt ggtggcttag gggatccggc gtggcggcaa
     5221 tctgctgcac ggcgttgacg tcaggcgcgc ggccagggta gcgaattgcc gccgtgccgg
     5281 ggatggtccc ggctgtgatg gccgcgtata acacccagag cgacaatagt agccccggcc
     5341 aaggccgccg gcctggtttt ctgggttctg acatgcggcc tattttactc gattatggcc
     5401 gatggcgcat gtctgacccg gccagccgcc cgaaagcatt caggacaggc tatggcccga
     5461 tgtatgtttg cctgcgagac gcaaatgcgt caaagtagta tacgccgcgc gtgccgctat
     5521 caatcccgga tacagcgcct agccggactc gatcaattcg ccgcgtgtcg ttgtctacgc
     5581 cgatcagatt ggcgcgctgc gcgccgtcaa tccacagcgt caggccccca ttgttcgcgc
     5641 cgccggcggt cgcggcgcgc cagtctattt cgacgaagtg aggcgcatcg ctgatgctga
     5701 accagctgct ggttttccag gtgctgccat cattgcgaag cgcaacccgg agttgataac
     5761 tgccattcga gtagcgaaat tggactcgcc ataccagcgt ggatgcgccg gtataaccat
     5821 agaagagata atgattatca ttgtcgatca tcgaaatcga gttcgggtcg aagtagaacc
     5881 gcgcccggta gcgcggctcg gcgctgggcc ggtcgtcggc gacataaatg gagttattgt
     5941 cgtctatcac cgccttcagc cccttgccgc cgatcaaggc cgccgcagcg ctcacgctga
     6001 ggtcgccgac atcggtcgtg ctcgatgacc aagctgacag gctacccgac tcgaagccgt
     6061 cggcgaagat caagtctgac ggggttgcgg tggcggttgg cgtgcgagtc ggcatggcgg
     6121 tcggtgtcgg ggtatcggtt ggcgtcgagg tgccagtcgg ggtagatgtc ggcgtgggtg
     6181 tatcggtcga tgtcggagta cgggaaggcg tgggcgtcgc cgagggagtc ggtgtgtcgg
     6241 tcggcgtcgg cgtgggcgta ggggttgacg tcggtgtatg ggacggcgtc ggcgtcgccg
     6301 atggagtgag tgtatcggtc ggagtcggtg ctccggcaag gacggtaaga ttggacgtta
     6361 aaatattgag accggtggcg gtgtcccata cgttcgtatt ggccagtctc actgcatacg
     6421 ccggggtgtt acgcaacgac gtaaattgat ccggcaacca caagctcagc gcataagtgc
     6481 cagagacaat attggtaggc acgggagcac tcacggtgat ggtgtgatcc cgtccggctt
     6541 cccaccgacg cggatctata ctagggagcg ggatgtcata tcggttgttt gcattatgca
     6601 acaccacata cactggcctg ggattgtaca gggatgcata cccgtcattt cttaaaacca
     6661 cgtccatatt cagtgcgccg cccggctgaa tggtttgcgg gacaacggct tctttgagta
     6721 ccagacgata gccgagtcga cgccggatgg tatccataca ccccccagtc acccaaccgt
     6781 ccagtacctc ttgtctgtaa tcgttattaa taaatgacca atgcagcatt tccaattcca
     6841 gttgtgcgtt cgggcattcg gtgcgcgggg ggttaaccgc gcaggtttct ccaccgacag
     6901 gcgtaaagcg cccttcttgg gcgacgaacc ctttccagca ggcaatttga tcttgacctg
     6961 cacagtatag tgattcatgt tgtggctgct gtaatgtctg tgactgatag gtgccttcat
     7021 catcatcgcc gcttagaaaa caatcattat ggtggccaat gcggcttgca tctgtttgtg
     7081 aaaaagcttg tgattctgtc aatggcccct ggaagaatat ttctttgtac cgtggatacc
     7141 gaagcataag catgcgctgt gtgggcaaca gttgaagaag cgcatcaaca attgctcttc
     7201 tatactcaag tggatgaagg tattttgaac tgtgccactc accccaatga ccaacaaaac
     7261 ccgcttgcat gacggcaatc acatcggcat tttcaatgag tatgggtctg agttggtcta
     7321 tgtgttgaag cacgaggtcg ataggcgcat cagggttggc taaatctggg tcatttgtcg
     7381 agcccgggcc atcgttatag ataaaccgga taaccaactt aagcccctta tcccttgcag
     7441 tttgaaaagc agagctaacc gtagacaagc cgttttgatc gatcggcctg tctctgtagt
     7501 tatctagccg aaagtatatc cagacgagcg agtcagaagg attgacagcc ctgatctttg
     7561 tcggatctaa gggttgatca atccagatcg atgattgttt cataaacccg cgttcagggt
     7621 tggcaaagtc ttcggttgag ggtgtgtact ggatgtgggt ttgattcgta gcaataggtg
     7681 tcggcgtatg acttggcgtc ggcgtctcag tcggcgtcgc cgtctcggtg ggtgtcgcgg
     7741 ttggcgatgc gtcaagcgta tacgtgtcga tcaccgcgcc cgtgcgcgcg atgaactgaa
     7801 agtcgatctg cgtttcactg gcctcgacga gcatcgcgcc gtagtcgccg ttgtagcgca
     7861 cctggctgcc ggacaccggc gtgctgaagg tgtagatgga cgcgccgccc aggccattga
     7921 cgaagtaggg aatgccatcc ggcaggatac gctcgtacgt gtgatcgtgc ccggccagca
     7981 ccgcgtccgc gccccacgct tgatacggcc actgcatgac cgtcgtggag ccgtgagtcg
     8041 cccccgacga ataaggtgga tggtgcatgt aaaccagatt ccacggtgag gtggaggcag
     8101 ccagttgcgt ttgcagccag atgccctggg tggaggcgct cgagtttcca tccggttctc
     8161 gaccgtcact gtcgacggcg aagaaatgca ccggccccca ggtgaaatcg tagtagcgct
     8221 cgttgccggg cagcgtgaag taatccagat acggttggac gttcggcgtg ttccaatcgt
     8281 gattgcccag cgaggggaag aagcgattgg tcgtcgcgcc ggctccgtag gatccggtgt
     8341 agggataaat gaactcgtga taatactgcc cgatgttcct gtctatcgtc tcagccgcgc
     8401 cgtccgggta gttgttgtcg ccggtggtga tgacgaagtc ggggttccag cctttgacaa
     8461 ggtcagccac atcctgctca gcctggctgt cgctgccgta atcgccgatg acggcgagcc
     8521 ggatgactcc ggccggaaca ggcgtgagag tgggtcttgg agttggggtt ggggttgaag
     8581 ttggagctgg ggtaagggtc ggcgagacgg ttgccgggcc aatgtaagtg aaccggcggg
     8641 attcaaaagc gtcgaaggtg taggttccgc gggtcccggc atctatcccg ttgagcgccc
     8701 ctaaccgggc gcgatcgatg cgccaggtgt cgttgtccct tccggtcagg ttggcttgtt
     8761 gtgttccatc tatccataga gtcaagccac catcattcgc gctcatcgct gtcgccgccc
     8821 gccaatccaa ctcgataaag tggggcgcat cactcagggt gaaccagttg gcgctcaccc
     8881 aagtgacact atcgtccaag agcagggcag cacgaagctg gtatatccct gaggactggc
     8941 ggaattccac tcgcaagacc tccgtggatg tgcccatgaa gcctttgaag atgtaatgag
     9001 catcgccgct tgccattgga atcgagttcg ggtcgaagta gaagcgcgcc cggtagcgcg
     9061 gctcggcagt gggacggtcg tcggtgacgt atatcgtatt gctgtcatcg atcaccgcct
     9121 gcaagccttg actgtccatc agcgcagcca cggcgctgac actgagatca cccagatcat
     9181 tactattgga tgtccaggca gacaaatcgc ccgactcgaa gccgtcggca aagatcagat
     9241 cgggcaaacc tggcgtgctc gtcgcggtgg gtatggccgt cggcgtaagg ggctgattag
     9301 gatcgaattc cttccagccg aggtcgggag ccaaaccgga gaagtttgtg atcggggcgg
     9361 gtgggacggc ctctccatca ttggcgatgt attgcgtgac acccgcatca atcgcgggag
     9421 atccgggctg gaggatcaat ccactgaaat catcgtccac cgttccccat acattatcgg
     9481 gaccggcgtt cggtggggat acgaagaggg gattctgccc gaagaggttt cccgcgccga
     9541 ggtcgctctg atcggtatcc aggctgttgt tatgaaacaa ggtgtatgcc accacggagt
     9601 tggctcccgc caggccctgc acccgccagg ctcccttcgt gaccgagttg gcgatcatgt
     9661 tgttgaaagc caccaggtta tccccaccgg atatgccata gtcattgcca tagaaggtat
     9721 tgttgtagac tcgaatagcc tccacgatat tcgcaccgga atagtcctcg gtgctggcct
     9781 ggttgggtat gaggccgatg ccagccttgc ggcaattggc gatcaggttg ccgcggatga
     9841 caaaacggcg gttgctgtcc agcggctggc tgtcgtcaat cagttggatg ccatcttcat
     9901 tgctcccgat aatctcattg ttccggatcg tgatctcaat aggctgggcc ggcgcgctga
     9961 ggtcttgcag ccgaatctcg attccgtcgt ccacgctgta catcatgcgg ttgttctcga
    10021 tgaccagggg gcggttcata ttgtccaggt cgatcgaatc atcccctgag tggaaaaaca
    10081 cattgtagcg gttgatgccc ccgctgtcct gtccatagtc gttcaggtcc cccaccgact
    10141 gcaggaggta gttgtactcg acgatgctct ccgagcgtat cacgatgcca tcgctcccgt
    10201 tgcgaatgac aaagccccgg atggtcggca tcggggagag gccggcaggg atggcgatgg
    10261 tggccaccag actcgacggc ccgccgtcga cgatcgtcgt gttgcgagta gggtctgtgg
    10321 cgtcgaagac ctcggcggtc aggatgacag acttgttgag cgtcagattc tcaaggtacg
    10381 tgccgggacg gacgaggatg gtatcgccgg ggttggcggc gtcgatcgcc gcctggatcg
    10441 tggggtaatt atcggggacg atcagcgtcg ccgcccggac gctctggtaa accgcgagcg
    10501 cggcgatggc ggtgagaatt ccggctgtga gaaagaaaaa cttacgcaca ctgcacatca
    10561 tcggaagtct agctcacaaa cgactgaagt cgtcactagc cacccatttg gatatcaaat
    10621 gtctgcaact gacccgactc gctcagttca tcattcgcgc gctgaagcaa cgttatggtt
    10681 gcgttcgaga aattgaggcc ccggcaatgc ctcggccttg aaaattggat ctagtgagtc
    10741 tggaagcagt ggcgtaggtg ttgatgactc ggttagcatg tagtatatga tctgattccc
    10801 ttacactctt gtcaaaccgg cactcccacg acgggggcga gcttcagatt cgataagcgc
    10861 gcgcggaaga tgggcaagtc atgaatcgcc accgacacgc atcgcttgag ccaacgcaga
    10921 ccgagttgaa acagactcag ttcgcgtgtg gggcctggat caatctgccg acgcgcggat
    10981 tcaccgtggg cgagcacccg ctcgcccagt tcgtgacacc agagcgtggc gacggccatc
    11041 gccaacagca accgttcgag gcggtcggca tgttgcaggc gcgtatgctc cagatcgaag
    11101 ccgccggact tatcatcgcg gaagctttgc tcaatactca tgcgacggcc gtactcccgc
    11161 aagcgcgcgc ggcaggcact ctgatccgat atcaccgcca acagttcggg ttcggtcttc
    11221 gcatcgcctt cggtccagcc gaccgacagg ttggcccgcc acagcccctc ttgggtcacg
    11281 cacacgtttt gaaagtagcg gcgctggccg cgtggtacgc ccaactcatc cacgcgacag
    11341 gcgcggccat cctccagggt gatgtaggta ttgcaggtga tgcgcagatc gtaatgccag
    11401 cccaatttcc gggccaattt ggcccaatca cagtcgcgaa agccacggtc ggccagaaag
    11461 gtgacccggt gcacccgtcg gcgcagaaac tgggcgacac ggcccaaccg ctcggccagt
    11521 cgctcgaccg cgatcacccc ggtgccgtcg acgaccatcc acatcagggg aatcgcccga
    11581 cagccgtgtt gcaacgacac ccgaaacact tgccaacggt cgccgaagac catcacgcca
    11641 tccaaaatga cgaacacgtc gaccgctcgc caaccgtgca gcacgtgcgc caggaggggt
    11701 tcatagaaag cccacacatc taccttcagg ttcttcagcc agcgccggat caacgtcacg
    11761 cgcgactcgg cctgggtgtc catgggcaga tacgtcgcga ttttgctcaa cgcgggcgag
    11821 ttgcccagca ccagaccgac gacaatccac agccaattgg tgatctgctt gctgttcgac
    11881 acgctaacca gtggccgaat cgtttcggct aatcggttgt acaattcttg gctaatgctc
    11941 atgggcctgc ggctcctttg gtcgtcagat aaccaaatta tgccgcatcc catgagcagc
    12001 gccgttttag acttctgtca gggaatcagg tggaggtcaa cagcgatgca agctgaccga
    12061 taccaacgcg ctatactgcg cgtaggctcc tatgtgtcca cttcgtccag cacgatcacg
    12121 cgcagctcga tgatgcgctt gagcgcggta tcgttggtca agaacgcatc acagcccgcc
    12181 gccaacgcta cggagacttg aaaggcgtca ggcaagttca agttgtatcg ggcacgcaac
    12241 tcagcggctc gatccgccac atcgcgatcg atgacgacaa agctcgtgtt cgccccgttc
    12301 agaatgagtg cggcaaactc tcggccaatg tccaaacgtc catcgcgata cggcacgacc
    12361 aggcattcgg ccagtgtcac cggcgaggtc acggccataa agtcgccccg atccagccgc
    12421 tcgaagacag gttcaaccaa tgctagatag cgcgcctgtt cctcgacgta atagaccaaa
    12481 ggcgcggtat ccaagaatag gcgggcgaca tcgcgaaggt gatcggcaat cttcatgatt
    12541 gccgcctcaa ttgctgctca cggcgctcgt cgccttcacg ccgggtgcgt gagacccaag
    12601 cctgcgcgtc ttcgccaaat aacgaaggcc gcgctatgcc gcgaatctcg gcccatttgc
    12661 ggcgtggttc tgccaccggg taagcggtgc gtgctttgtc gaccaggtac gtcgccagcc
    12721 gcagttgctc atcgggcgtc agcttatcga cctgcttaat catatcgttc agcaaatcgg
    12781 cactcatcgt cacttcctcc ttctcccgta atgtcctgtg ccggccggcg agtcgtcaaa
    12841 ccccatgccc caacctcgac aggttgtctc gaatcgcctg ctccagccgt gctgactcca
    12901 cgaactgttc ttcccgtttc gccgtcagcc tctttatttt atcatcaaac ggctcgtcat
    12961 catcttcgac ttcctcctcg ccgaagtagc gccccggcgt cagcacaaag ccatgcgctc
    13021 tgcacccgcg tcctcgctcc gtccctcccg atcctgccgt acgacgagcg cgcccgcctg
    13081 acgcaaacaa acagaacgcc cgctcactcc attggcgagc gggcgatccg gtgctcttcc
    13141 ccacccgggc aatcaccgcc tcagccggta tccgggccac gtgcctgacc gggcgcggcc
    13201 ttgcgctgat gtgtctgttt gacgcgcttc agccgaaagt gat
//
LOCUS       DBNHXB010000431         3933 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3989660, whole genome shotgun sequence.
ACCESSION   DBNHXB010000431 DBNHXB010000000
VERSION     DBNHXB010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3989660"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..599)
                     /locus_tag="AC162G_17000"
     CDS             complement(87..599)
                     /locus_tag="AC162G_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077558.1"
                     /translation="MLIIVGVSVLGFLYVATAVSTSDFLWFTPTFSERANVIVVHCAG
                     EVMTYAPGSTEFEALNASVNADLSGSKSYLEAGLSQQTQEDYWKQHLVLEVVYPQPVR
                     MHVSFRYGLFDTLIYPVTGTFSQEGYVFGRRGETVLTGVDVGDGERSVAQTLKDLGYT
                     WEGYRGCTKP"
     gene            complement(782..1714)
                     /locus_tag="AC162G_17005"
     CDS             complement(782..1714)
                     /locus_tag="AC162G_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019010985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077559.1"
                     /translation="MSTAELELQAEPLLTTEAESQFRVIWKRFRRHKLAMAGIVIIAF
                     FVTMAIVAPFAAPYDPIRFQDLSNRNAPPSAEHLLGTDKIGRDILSRLMFGASISLTV
                     AITVVFMSETFGAILGAISGYYGGRVDNLVQRLTEFMLSLPLLPMLLAVSAIFKDQFS
                     VPGLPREWNSAVLIIVVLTVFGWMGACRLVRGQVLSLRAQDFTEAARALGVGNRRIIL
                     RHMIPNSLPPIIVNATLNLGFVIVIESALSFLTFGVQPPVPTWGNMLADYQAELWTEP
                     VKVFFPGLSIFLCALAFNYIGDGLRDALDPRLKR"
     gene            complement(1768..3222)
                     /locus_tag="AC162G_17010"
     CDS             complement(1768..3222)
                     /locus_tag="AC162G_17010"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077560.1"
                     /translation="MTAYIIRRLIQAFFVVIAATIVIFMLLNAAPGGPLSGLRIGSTD
                     AKSRVTEAQIRQLENLLGIDKPVGLQYLAWVIGDDWMGADWMYAGLERYPVSAKTAVR
                     FWADPGVAHIKPNYDIWVRGTETTPGEIHAVYIEAKPKGQRPDDMIGAKVEKVLGSNL
                     EVTVGGTRKALIITAPETEFVISGVDPRPEEGSWINISGLTGAYGLLGQWAGFHGEKA
                     GVARMDWGTSWSVANGQPVSAIVESRLSNTLVLMISATLLSLLVGIPIGVYSAVHQYS
                     KADYAITTFSFFGTSMPVFWFGMMMILIFSYGFGGLGLPFMPAGGVESVRSAPEGSVL
                     NLLGSQPGDLIDRFMHLLMPAIVLSLLYLAGWSRFTRSSMLEVLRQDYVRTARAKGLR
                     ERIVIYKHALRTALIPVVTVVVLQIPGIFGGAILTETVFNYKGIGRLYFESLGRSDWP
                     LVMIILLISTVLTVLSTLLGDVLYTVIDPRIRFH"
     gene            complement(3499..>3933)
                     /locus_tag="AC162G_17015"
     CDS             complement(3499..>3933)
                     /locus_tag="AC162G_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077561.1"
                     /translation="GIEVTLENLSAAEYFADGPDGPLFGRAYDVGSFTWLTGVVPSCD
                     LYITDQIPAEENNWGGQNNPGFTDEEYDAACKQALGLLYGTDAYVEAQKEAQRLFSEK
                     LPVVPLFLRLKIAAYRPEVVGFIMDPTENSEMWNIENFDLSQ"
BASE COUNT          776 a         1285 c         1191 g          681 t
ORIGIN      
        1 cgcacgcggc acactgtggt agagatgcct cgtcgcgcta cgcgctcctc ggcatgacac
       61 gacacgcagg cgtgcctcac tccgcctcat ggcttcgtgc agccgcgata accttcccag
      121 gtgtaaccca ggtccttcag ggtctgcgcg accgaccgct cgccgtcccc gacgtcgacg
      181 ccggtcagca cggtctcgcc gcgccgcccg aagacgtagc cctcctgcga gaacgtgccc
      241 gtgaccgggt aaatcagcgt gtcgaataag ccgtaccgaa acgagacatg catccgcacc
      301 ggctgcggat agaccacctc cagcaccagg tgttgcttcc aatagtcttc ctgcgtctgc
      361 tggctcagtc ccgcctccag atagctcttg ctgccggaca gatcggcgtt gacgctcgcg
      421 ttgagcgcct caaactcggt cgagccaggc gcgtacgtca tcacctctcc cgcgcagtgc
      481 acgacgatca cattggcgcg ctcgctgaag gtcggagtga accacaggaa atcgcttgtg
      541 ctcacggcgg tcgcgacata caggaagccg agaacgctca cgccaacaat aatcagcaaa
      601 gaaaccacgt tcgaggttct ttgccgaatg ctatcgggat cattcacgag acgtaactag
      661 tcacccgcgc ttcggagttg cccacgaagc cacacgaagc gacacgaagt tgaacattcc
      721 ttcgtgaaga ttcgtgtgaa ttcgtggaag aaaaaagacg acctgagcag tgaccacgag
      781 attatctctt caggcgcggg tcgagcgcgt cgcgcaggcc gtcgccgatg tagttgaacg
      841 ccagggcgca caggaaaatg gacaggcccg ggaagaacac cttgaccggc tccgtccaca
      901 gctcggcctg atagtcggcc agcatgttgc cccaggtggg caccggcggc tgaaccccga
      961 acgtcaggaa actgagcgcc gattcgatga cgatcacgaa gcccaggttc agcgtagcat
     1021 tgacgataat cggcggcagc gagttcggga tcatgtggcg caggatgatg cgccggttgc
     1081 ccacgcccaa cgcgcgcgcg gcctcggtga agtcctgcgc gcgcagcgac agcacctgcc
     1141 cgcgcaccag gcggcacgcg cccatccagc cgaagacggt cagcacgacg atgatgagca
     1201 ccgcgctgtt ccactcgcgg ggcaggccgg gaaccgagaa ctggtctttg aagatcgccg
     1261 acaccgccag cagcatcggc agcagcggca gcgagagcat gaactccgtc aggcgctgaa
     1321 cgaggttgtc gacgcggccg ccgtagtagc ccgagatcgc gcccagaatc gctccaaacg
     1381 tctcgctcat aaacacgacg gtgatggcga ccgtcagcga gatgctcgcg ccgaacatca
     1441 gccggctcag gatgtcgcgg ccgatcttgt cggtgcccaa caggtgctcc gccgacggcg
     1501 gcgcgttcct attggacagg tcctggaagc ggatcggatc gtaaggcgcg gcgaacgggg
     1561 ccacgatcgc catggtgaca aagaaggcga tgatgacgat gccggccatc gccagcttgt
     1621 gccggcggaa gcgcttccag atcacccgaa attggctctc ggcctccgtc gtcaagaggg
     1681 gttcagcttg aagttcgagt tccgcagtgc tcattcgcaa tctcctcaag acctcacagg
     1741 tctcggagac ctgtgaggtc tgtggaacta gtggaaccgg atgcgcgggt cgatcaccgt
     1801 gtacagaacg tcgcccagca gcgtcgacag cacggttagc acggtcgaga tcagcaggat
     1861 gatcatcacc agcggccagt cgctgcggcc cagcgactcg aagtacaacc ggccgatgcc
     1921 tttgtaattg aagaccgtct cggtgaggat cgccccgccg aagatgcccg gaatctgcag
     1981 gacgacgacg gtgacgaccg ggatcagcgc ggtacgcagc gcgtgcttgt agatgacgat
     2041 gcgctcgcgc aggcccttag cgcgcgccgt gcgcacgtaa tcctgccgga gcacctcgag
     2101 catcgacgag cgcgtgaatc ggctccagcc ggccaggtac agcaagctga gcacgatcgc
     2161 cggcatgagc aggtgcatga accggtcgat gagatcgccc ggttgcgacc ccagcaggtt
     2221 gagcacactc ccttccggcg ccgagcgcac gctctccacg cccccggccg gcatgaacgg
     2281 caagcccagc cctccgaacc cataagagaa gattaagatc atcatcatgc cgaaccagaa
     2341 caccggcatc gacgtgccga agaagctgaa ggtcgtgatc gcgtagtcgg ccttcgagta
     2401 ctggtgcacc gccgagtaca cgccgatcgg gatgccgacg agcagcgaca gcagcgtggc
     2461 gctgatcatc aagaccagcg tgttagacag ccggctctcg acgatcgccg ataccggctg
     2521 cccgttagcc accgaccacg acgtgcccca gtccatgcgc gcgacgccgg ctttctcgcc
     2581 gtggaagccg gcccactgcc ctagcaggcc gtacgcgccg gtcaggccgc tgatgttgat
     2641 ccagctccct tcctctggcc tgggatcgac gcccgagatc acgaactccg tctcaggcgc
     2701 ggtgatgatc agtgccttgc gcgtgccgcc gaccgtcact tcgagattag agcctaacac
     2761 cttttcgacc ttcgcgccga tcatgtcgtc gggcctctgg cctttgggct tggcttcgat
     2821 gtagacggcg tggatttcgc cgggcgtcgt ctccgtgccg cgcacccaga tgtcatagtt
     2881 cggtttgatg tgcgcgacgc cgggatcggc ccagaagcgc accgccgtct tcgcgctcac
     2941 cgggtagcgc tctaagccgg cgtacatcca gtcggcgccc atccagtcgt cgccgatcac
     3001 ccaggccagg tactgcagcc cgaccggctt gtcgatgccc agcaggtttt ccagttgccg
     3061 gatctgggcc tcggtcacac ggctcttggc gtcggtgctc ccgatcctca agccagacag
     3121 cggcccgccc ggcgcggcgt tcagcagcat gaagatcacg atcgtcgccg cgatcacgac
     3181 gaagaaagcc tgaatcaggc ggcggatgat gtacgcggtc atggcttcac tcctcagaaa
     3241 cctggcttct ccaatatact cacgatacgc ggcgaaggtt caggcagttt atgtatagat
     3301 tcagcagatt gcggcgtgtc attccgaacg ccccaaccgc gtcattccga acgcagtgag
     3361 gaatctctgc tccagccgac gagcgcggcg tgccggtgta gagatgcctc gtcgcgcctc
     3421 ctcggcatga cacgcctacc acttagagag atggccgcgc gtgcgcggcc atctcttcac
     3481 tcggagctcg accctcgatt actgggacag gtcgaagttc tcgatgttcc acatctcgct
     3541 gttctcggtc gggtccatga tgaagcccac gacctccggc cgataggccg caatcttcag
     3601 gcgcaggaac aacgggacga ccggcagttt ctcggagaag aggcgctgcg cttccttctg
     3661 cgcttcgacg taggcgtccg tgccgtacag caggccgagc gcctgcttgc aggcggcgtc
     3721 gtactcctcg tcggtgaatc cggggttgtt ctggccaccc cagttgttct cttccgccgg
     3781 gatctggtcg gtgatgtaca ggtcacacga cggcacgaca cctgtcagcc aggtgaacga
     3841 gccgacgtcg taggcgcgcc cgaacagcgg gccatccggg ccatccgcga agtactcagc
     3901 ggccgacagg ttctccagcg tcacttcgat gcc
//
LOCUS       DBNHXB010000432         7047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3149450, whole genome shotgun sequence.
ACCESSION   DBNHXB010000432 DBNHXB010000000
VERSION     DBNHXB010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3149450"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1172)
                     /locus_tag="AC162G_17020"
     CDS             complement(90..1172)
                     /locus_tag="AC162G_17020"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HIR5077562.1"
                     /translation="MKLQDLQFDLPDEKAASVPPEAEGRSRSDVRLLVIDRKSGLIEH
                     RKFVNLPEYLRPGDVVVLNASATLSSQFVATDTLGREIKVRLSSRDPASNGRGDKWHA
                     VLDPDAEIRPGDVLRFGVIEGKELKELKELRATVVTRHPRVPKLWLLVFDLQGTSIDE
                     WRSRLGKPIRYHYVPQEWELGYYQNVYAQFPGSSEMPSAGRAFTPEMLTELTDRGIGV
                     VRIVLHTGVSNIDIEEEQVEQHVMYEEPYQITDEAAQIINHARERGGRVFAVGTTVVR
                     TLETVADERGYLEGRRGWTSLYITPGYRFKVVDALVTGLHEAKSTRLVLATAFAGDKD
                     LVLRAYHEALERDYRWHEFGDLTLIL"
     gene            complement(1147..1887)
                     /locus_tag="AC162G_17025"
     CDS             complement(1147..1887)
                     /locus_tag="AC162G_17025"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5077563.1"
                     /translation="MLSDKVVLITGGSRGLGRALAKAFAAEGARVVIAARNPETLEAA
                     RREIESLGGEVLALAADMTQPADISHVVAAAIERFGRIDVLINSAGTLGPTPRPSLLD
                     TDPDALLETFRVNTAGPLRVTQAVLPHMLRQGRGLVINVTSDAARGAYPEWGAYGASK
                     AALELIGLTWAAELEGTGVRVVNVDPGDLQTDMQQAAFPGEDISDRALPESAAPAFVW
                     LAAEAGSYVNGERVQAQEFKHETPRPAI"
     gene            complement(2125..3888)
                     /locus_tag="AC162G_17030"
     CDS             complement(2125..3888)
                     /locus_tag="AC162G_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012408125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR5077564.1"
                     /translation="MTAHSEYLVRAARLTDVQASGCLSVQVNGHTLALFAYGDKVYAV
                     DNRCPHMGFPLDRGTLKDGILTCHWHHARFDLASGGTFDQFADDVHVFPVEIRSGQVW
                     VDVAPRGDPRAHQRDRLQVGLDRDIPLVLGKAVITLLDGGGDAVDPFRVGLDFGARYR
                     QAGWGQGLTMLTCLMNLLPYLNAEDRPRALYHGLSAVALDCDGSPPRFPVRPLPDSTT
                     DIATLKRWFRQFVEVRDAEGAERCIVSAIRAGADHRQLADILFAAATDHRYIQIGHVA
                     DFTNKALEALDAAGWDRPRAEAVLTSLASGYAEADRMEESNAWRNPIDLIEILDRTFD
                     ELPAVLETGRTHRDGPWTGRDALAPILLGEDPQAIAGALLGALREGCTEEQLAGAVAY
                     AAALRIARYHTSNEFGDWDTALHTFTFANAIHQGLRRAPSVELLRGVFDAAMSVYLDR
                     FLNVPAAKVPEPESLPKVSETSEALLAELPSLLDRQQQVNQAGEVVARYLYSGGDPAR
                     LLAMLGHLLLREDRNFHTIQTIEAAFRQYALLRGTPASAHVLVAAARYLAAHAPTVRA
                     QGQTYQIAYRLHRGERLFEEA"
     gene            complement(4021..4683)
                     /locus_tag="AC162G_17035"
     CDS             complement(4021..4683)
                     /locus_tag="AC162G_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077565.1"
                     /translation="MENASPVLWACVGPFALLMAWLAFRSTVRWKSRQYLVSGRPKTL
                     GTFVYSAIFNPIRRSMGIRPLRVIPHVDDAHRSEVEMLERADRLAREVRAALKHGPMS
                     ALRKSSLERQVREIPDNLVRAMWTLARLRRVYDSLDPRFDRDGQNRREITEMQNKLFA
                     EMNHSVEVLSNIPVSLIKVELARSDGSVDKVLVELDETNKRLLDLSASYTEVKQWGQS
                     TS"
     gene            complement(4724..>7047)
                     /locus_tag="AC162G_17040"
     CDS             complement(4724..>7047)
                     /locus_tag="AC162G_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077566.1"
                     /translation="LRRAIPSQGLLSVWWRSILNLVRLLRNLLTLLVLVPLRGLYMAL
                     SRIPMLRALVVGIMRLFDGLRSFLDRVVAGIGQLIRRLRILSIQAVRKAAVALRQLAL
                     GIGRLLARLGNALNRLMRLVHAALSRLAGFVTAAFDRLRSAVAWAFDRLSDLLVGLLH
                     GLRTVIAWIGEIVSTVLRDLRRAIASVLAFIGDLLATLLRSVRDILSKVVEGIAHAFH
                     ILRRGIDWILARLGDALAALIRVLRAVLSKMAEAMSYVLRAARRGIAWLLAFLGDALD
                     ALFQFLRAALSKVDEALRAAFRILWRAVRWVVARVDKALSSLLHAVRLVLSKAARIVV
                     SALRALAVWLKQMFRRLWNVARRLFAWIRLMLGLARAALSKIARLALSALRVVIVWIK
                     WPFRQVWTLLRHLMAWLRLVKHELGRVLAKVVRFVRSTLRVLVVWAKWQVRQLASLVR
                     GLAAWLRRLAGQLRIVLLRTARIIASTLRIAAEWIKRPLLHVWRLVKYLATWLGKLFR
                     ALDVVFTAIGERVIRGLQIAWAGIRQVLARVGEVLRTLWRAMHFALVKIGAGIRIVFR
                     ALRIGVTWLLARVGDVLRILWRAIRLVLAKIGAGIRIVFQVLREGMRWLLARVDDALN
                     ALLRAMQFVLSQIAEAVRFALRLLTTGITWLLDHVRDALVGLLRLVQLVLSNLAEGIS
                     AVLRVLKTGILRVLDGLRDLLVSLIRGAQALVSRVAAGVALVLSWSVTGATLIFNFVR
                     DLLKLFLEQANQLYKRLNDRLETTIDQIRTGMR"
BASE COUNT         1446 a         2312 c         2133 g         1156 t
ORIGIN      
        1 ctatcgacct ggcggcacag aaaacgcagc ttgtgatagg ctgaggaaaa accctctcgc
       61 aggtcctcaa acctgcggga gtgtttgtgc tacaaaatca aagtgagatc gccgaactcg
      121 tgccaccgat agtcacgctc gagcgcttcg tggtaggcac gcaggacgag atccttgtcg
      181 ccagcgaagg ccgtggccag caccagccgc gtcgatttcg cttcgtgcag gccggtcacg
      241 agcgcgtcga cgaccttgaa gcgatagccc ggcgtgatgt acagcgaagt ccaaccgcgc
      301 ctcccttcaa gatagcctct ctcgtcggcg accgtctcga gcgtgcggac aaccgtcgtg
      361 ccgacggcga agacccgccc gccgcgctct cgcgcatggt tgatgatctg cgcagcctcg
      421 tcggtgatct ggtacggctc ctcgtacatg acgtgctgct cgacctgttc ttcttcgatg
      481 tcgatgttcg acacacccgt gtgcaggaca atgcgcacga cgccaatgcc ccgatctgtc
      541 agctccgtaa gcatctccgg cgtgaaggcg cgccccgccg aaggcatttc ggatgatccg
      601 gggaactgcg cgtagacgtt ctggtagtag ccaagctccc actcctgagg gacgtagtga
      661 taacggatcg gtttacccaa ccggctgcgc cactcgtcta tcgacgtccc ctgtaaatca
      721 aacaccagaa gccacaactt gggcacgcgc ggatggcgcg tgacgaccgt cgcccgcagt
      781 tccttgagtt cctttaattc cttgccctcg atcacgccaa atcgcaatac atctccaggt
      841 cgaatctccg cgtccgggtc gagcacggcg tgccacttgt cacctctgcc gttcgaggcg
      901 ggatcgcgcg acgacagccg taccttgatc tcgcgtccga gagtgtccgt cgccacgaat
      961 tgcgaagaca acgtggcgct cgcgttgagg acgacgacat cgccgggacg aaggtactcc
     1021 ggcaagttga cgaacttgcg atgctcgatc aggccagact tgcggtcgat gaccagcaat
     1081 cgcacatcgg atcggctccg tccttcggct tccggcggca ctgaggccgc cttctcgtcg
     1141 ggaagatcaa attgcaggtc ttggagtttc atgcttgaac tcttgcgcct gcacccgctc
     1201 gccgttcacg tatgaccccg cctcggcggc cagccacaca aaggcgggcg cggcgctctc
     1261 cggcagcgcg cgatcgctaa tgtcttcgcc ggggaaggca gcctgctgca tatccgtctg
     1321 caagtcgccg ggatcgacgt tgacgacgcg cacgcctgtg ccttcgagtt ccgccgccca
     1381 cgtcaggccg atcagctcga gcgcggcctt gctcgcgccg tacgcgcccc attcgggata
     1441 cgcgccgcgc gccgcgtccg aggtgacgtt gatcaccagc cccctgccct gcctgagcat
     1501 atgcggcagc acggcctgcg tcacgcgcag tgggccggcg gtgttgacgc ggaacgtctc
     1561 cagtagcgcg tcgggatcag tatcaagcag cgacgggcgc ggcgtcgggc cgagcgtgcc
     1621 ggcgctgttg atcagcacgt cgatacgccc aaagcgctca atcgctgcgg cgacgacgtg
     1681 ggagatgtcg gccggctgcg tcatgtcggc ggccagcgcc aaaacttcgc ctcccaacga
     1741 ctcgatttcg cggcgcgcgg cttcgagcgt ctcaggattt cgtgcggcga tgaccacgcg
     1801 cgcgccttcg gcggcgaacg ccttcgccag cgccctaccc agcccgcgcg atccgccggt
     1861 gatgagaacc actttatcgc tcagcatgct ccacctcttt cttcaattcg gcctcattga
     1921 taaccatttc ccggttgggc atatggccca acaggttaaa gcataggcca aaactatacc
     1981 gaccacatcc ggcaagtgga cggaatcgag gcaaagaaaa aggacaggcc ggagcctgtc
     2041 ctggggtaca gtttagacct gccaggtttt gaatgtaagt taaccgacca actttgtcaa
     2101 taaacccggc aggtctcaaa catgctacgc ctcctcaaac agccgctcgc ctcgatgcag
     2161 ccgataggca atctgatagg tctgcccctg cgcccggacg gtcggcgcgt gggcggccag
     2221 gtagcgcgcg gcggcaacca gcacgtgcgc actggccggt gttccgcgca gcagcgcata
     2281 ctggcggaag gcggcttcga tcgtctgaat cgtgtggaag ttacgatcct cgcgcagcag
     2341 cagatggccg agcatcgcca gcagccgagc cggatcgccg ccgctgtaca ggtaacgcgc
     2401 gaccacttca ccggcctggt tgacctgctg ctggcgatcg agcagcgacg gaagttcggc
     2461 caacaaagct tccgaagttt ccgaaacttt cggaaggctc tccggctcgg gcactttcgc
     2521 cgccggcaca ttcaagaagc gatcgaggta cacactcatc gccgcgtcga agacgccgcg
     2581 cagcaactcg accgacgggg cgcgccgcag gccttgatga atcgcgttgg cgaacgtgaa
     2641 cgtgtgaagc gcggtgtccc aatcgccgaa ctcattgctg gtatgatatc gagcgatgcg
     2701 gagcgcggcg gcatacgcga ctgccccagc cagttgttct tccgtgcagc cttcacgcag
     2761 tgcgccgagc agcgcgccgg cgatagcctg cgggtcttcg ccaagcagga ttggagcgag
     2821 cgcgtcgcgg ccagtccatg gcccatcgcg atgcgttcgc cccgtctcca gcaccgccgg
     2881 cagctcgtcg aaggtgcgat caagaatctc aatcaggtca atcgggttgc gccaggcgtt
     2941 cgactcttcc atgcggtcgg cctcggcata gccggaggcc aggctggtca gcacagcttc
     3001 ggcgcgcggc cgatcccatc ctgcagcgtc gagggcttcc agcgccttgt tggtgaaatc
     3061 ggcaacgtgc ccgatctgaa tgtagcgatg gtctgttgcg gcggcgaaca atatgtcggc
     3121 cagttgccga tgatcggccc cggcgcgaat cgccgagacg atgcagcgtt ccgcgccctc
     3181 ggcatcgcgc acctctacga attggcggaa ccagcgcttg agcgtggcga tgtcggtggt
     3241 ggagtccggc agcggtcgaa ccgggaagcg cggcggcgat ccgtcgcagt cgagcgcgac
     3301 ggcggacagg ccgtgataca gcgcacgcgg acggtcctcg gcgttgagat agggcagcag
     3361 gttcatcagg caggtaagca tcgtgagacc ctgcccccac cccgcctgcc ggtagcgcgc
     3421 gccgaaatcc agcccgacgc gaaacggatc gaccgcgtca ccaccgccgt cgagcagcgt
     3481 gatcacagct ttgccaagga cgagcggaat atcccggtcc agcccgacct gcagccgatc
     3541 gcgctggtgg gcgcgcgggt cgccgcgcgg cgcgacgtcg acccacactt ggccgctgcg
     3601 aatctcgacc gggaaaacgt gcacatcgtc ggcgaactga tcgaacgtgc cgccactcgc
     3661 caggtcgaag cgcgcgtgat gccagtggca ggtgagaatg ccgtccttta gcgtgccccg
     3721 gtcaagcggg aaacccatgt gcgggcagcg attgtcgacg gcgtagacct tatcgccgta
     3781 ggcgaacagc gcgagcgtgt gcccgttcac ttgaacggac aggcagccgg aagcctgtac
     3841 atcggtcagg cgcgccgcgc ggacgaggta ttcggaatgg gctgtcatcg tttcacctcc
     3901 tcgaatggcg gccacatata tcgtattcgg caggcgaagg aagtcaacaa agaaaaagga
     3961 caggtttggg gcctgtccaa gggtacagta gatgcaatcc aacagcttgt ttcgtcgtga
     4021 tcatgacgtg gactgccccc attgcttcac ttccgtgtag ctggccgata aatcgagcag
     4081 ccgcttgttg gtctcatcca gctccaccag aaccttgtcg acgcttccgt cgctgcgcgc
     4141 cagctccact tttatcagcg acaccggaat gttcgagaga acctctacag aatggttcat
     4201 ctccgcgaag agcttatttt gcatttcggt gatctcacgg cgattctgac cgtcgcgatc
     4261 gaaccgtgga tcgagcgagt cgtagacgcg gcgcagtcgg gccagcgtcc acatcgccct
     4321 gaccaggttg tcgggaattt cgcgcacctg ccgttcgaga gacgatttcc tgagcgcgct
     4381 catcgggcca tgcttcaacg ctgctcgaac ctcccgcgcc aggcgatccg cgcgctccag
     4441 catctcgact tcactgcgat gcgcatcgtc aacatgcgga atcacgcgca gggggcggat
     4501 gcccatcgag cggcggatcg ggttgaaaat cgccgagtag acgaaggtgc ccaacgtctt
     4561 gggacggccg ctgacgagat actgtctgga cttccagcgc acggtcgacc tgaaggccag
     4621 ccatgccatc agtagcgcga acgggccaac gcaggcccac aagactggcg acgcattctc
     4681 catcgctcca acctccgtgt cgaaatatgc agagtgtccc gactcagcgc atccctgtcc
     4741 ggatctggtc gatggtcgtc tccagccgat cgttcaggcg cttgtatagt tgatttgcct
     4801 gttccaagaa cagcttcagc agatcgcgga cgaagttgaa gattagcgtc gctcccgtga
     4861 cgctccagga cagcaccagc gcgacgcccg cagccaccct tgagacgagc gcctgcgcgc
     4921 cgcgaatcag actcacaaga agatcgcgca ggccatccag gaccctgaga atgcccgtct
     4981 tcagaacccg taacacggca gagataccct cggccagatt ggagaggaca agctgcacga
     5041 ggcgcagcag tcctacgagc gcatcacgca cgtgatcgag cagccaggtt atgccggttg
     5101 tcagtaggcg cagagcgaac cgcacagcct cagctatctg tgagagaacg aattgcatgg
     5161 cacgcagcag cgcgttcagc gcatcgtcca cacgcgccag cagccacctc atgccctctc
     5221 gcaacacttg gaacacgatt cgaatgcctg cgccgatctt cgccaggacc aagcgtatcg
     5281 cgcgccagag gatgcgaagc acatcgccca cccgcgccag cagccacgtc acgccgatcc
     5341 gcaacgctcg gaaaacgatt cgaatgcctg caccgatctt caccagggcg aagtgcatcg
     5401 cacgccagag ggtgcgaagc acttcaccaa cgcgcgccag cacttgccgg atgcctgccc
     5461 atgcgatctg caatccacgg atgacgcgct cgccaatcgc cgtgaaaaca acgtccagag
     5521 cgcgaaacag cttgcccagc catgtcgcca ggtacttgac cagccgccac acatgcagca
     5581 gcggccgttt gatccattcg gctgcaatcc gaagcgtgga cgcgatgatt cgcgcagtgc
     5641 gcagcagaac gattcgcagt tggcctgcca gccgccgcag ccacgccgcg agaccccgca
     5701 ccaaactcgc taattgccgc acctgccact tcgcccagac gaccagtacg cgcagcgtcg
     5761 accggacgaa gcgaacgacc ttcgccagga cacggcccag ttcgtgcttc accagccgca
     5821 gccaggccat cagatgcctg agcagggtcc acacctgccg gaacggccac ttgatccaga
     5881 cgatcacgac gcgcagggcc gacagggcaa ggcgggcgat tttcgacaag gccgctcgcg
     5941 ccagccccag cattaaccgt atccacgcaa agagacgtct ggccacattc cagagccggc
     6001 gaaacatctg cttgagccaa acggccaggg cgcgcagcgc ggacacgacg atgcgggccg
     6061 ccttcgataa gacgaggcgc acggcgtgca gcagactgga cagcgccttg tccacccgcg
     6121 ccacgaccca cctgacggcg cgccacaaga ttcggaatgc agcgcgaaga gcttcgtcca
     6181 ccttcgacaa ggcggcgcgc aggaattgaa acagggcgtc gagagcatct cccaggaacg
     6241 ccagcagcca ggcgatgccg cgccgcgcgg cgcggagcac gtaggacatg gcctcagcca
     6301 tcttcgacag aacagcgcgc aggacgcgaa tcagggcggc gagagcatcg cccaagcgcg
     6361 ccaatatcca gtcgatgccg cgacgcaaga tgtggaacgc gtgcgcaatg ccctccacca
     6421 cttttgacag gatatcgcgc acggaacgaa gcagagttgc gagcaaatcg ccgatgaacg
     6481 ccagcaccga agcgatggcg cggcgcaagt cgcggagcac cgtgctgacg atctcaccga
     6541 tccaggcaat cacagttcgc agcccgtgca gcagacccac cagcaggtcg ctcagtcgat
     6601 cgaacgccca tgcgacggcc gatcgcagac gatcaaaagc agctgtgacg aagccggcca
     6661 accgggacaa agcggcgtgc acgaggcgca tcaggcgatt gagcgcgttg cccagccgcg
     6721 ccagcagccg gccgatgccc agcgctaatt ggcgcagcgc gacggcagcc ttgcggacgg
     6781 cctggatgga aaggatccgc agccggcgaa tgagctgccc aatgccagcc acgacccgat
     6841 caagaaaact tcgcaggccg tcgaacagcc gcatgatccc gaccacgagt gcgcgaagca
     6901 tggggatgcg ggacagggcc atgtaaagcc cgcgcagtgg gaccagcacc aacaacgtga
     6961 gcagattgcg cagcaggcga acgaggttga ggatagaacg ccaccacacg ctgaggaggc
     7021 cctggctcgg aatcgcccgc cgcagcg
//
LOCUS       DBNHXB010000433        12145 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2481656, whole genome shotgun sequence.
ACCESSION   DBNHXB010000433 DBNHXB010000000
VERSION     DBNHXB010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 12145)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12145)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12145
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2481656"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="AC162G_17045"
     CDS             <1..1082
                     /locus_tag="AC162G_17045"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR5077567.1"
                     /translation="YSHRDDEFVLRLATDLEDRGASVWIDQGNIQGGEQWRRSISTGV
                     QDSKAFVLVVSPDSINSEYVAEELASAFKHDRPIIPLLYRKTSIPAMLKAQLDAYQFL
                     DFGKGGYAQNLADLVAALARHGVALQAAPGEQARRRRERMGAPVPTNWGAVFGRIPGW
                     AFGWGLGWAIFWLVLPIVMTVLNQSELGNFPALPVGGFAGGIVGGLLAGLFTMLALRR
                     NAGSIRWKHMSRSIRIWGLVGPLGVIAAGALAVLLFNPDALLGNSPECSQVGLAECFM
                     QGIGYAIGAGLAAGLVLVLTIIFYAMIALFGVGLIAGWLAVRHIRRLEPGILGRQAIW
                     VVLGWGGGAVLAAIAGVAVMSLLGA"
     gene            1084..4575
                     /locus_tag="AC162G_17050"
     CDS             1084..4575
                     /locus_tag="AC162G_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR5077568.1"
                     /translation="MEFHVSRLARDRYQFDDMLFASNGNVIFANFHAARTFAQKMNAK
                     RDLARFPEQAIKAGQINALGLIDEILHYVVGQYRQQMSPNVMAQALAALDTQLGREAV
                     DAALRRFADEFPALAVYRREITLDAYLNGQTGDVPNRQIILEEMLMLWVANMNPACAP
                     FMELFDDTALEKETTYLRVVAGLREFFDTQPVFGPDNQNLIDMLRSPAVAVPHSLPGQ
                     LEYIQARWGLLLGRYLYRLLGSLDLIREEEKPIFAGFGPGEKGVPVYVFAGLEIEAER
                     FTPDREWMPRLVLIAKNAYVWLDQLSRRYQRPIARLDQIPDEELDTLARWGITGLWLI
                     GVWERSPASQRIKQLMGNADAVASAYSLYAYSVANDLGGDAALQNLKERAWRRGIRLA
                     SDMVPNHMGIDSPWVAEHPDWFISSDASPFPSYAFDSPNLSSDERMGIYLEDHYYSRS
                     DAAVVFKRVDHANGSVKYIYHGNDGTSLPWNDTAQLNYLKPEVREAVIQTILHVARQF
                     PIIRFDAAMTLAKKHYQRLWFPEPGTGGAIPSRADFGLPRRQFEAAMPVEFWREVVDR
                     AAVEAPDTLLLAEAFWLMEGYFVRTLGMHRVYNSAFMNMLRDEDNAKYRSVIKNTIEF
                     DPAILKRYVNFMNNPDERTAVDQFGKSDKYFGVCVMLATMPGLPMFGHGQIEGLAEKY
                     GMEFRRALWDEQPDSGFVQHHERLIFPLLHRRYLFADVENFLLYDLFTPEGHVDEDVF
                     AYSNRPSGIAGQAERALVVYHNKFGSTRGWIRMSAAYAVKTGEGGDRVLAQRTLAEGL
                     DIRDEAGFYTIFRDHVTGLEYLRSNHDLAESGLYIELGAYEHHVFLDFREVRDDERRQ
                     YAQLHAYLGGRGVPSVDEAMRELFLQPIHRAFADLVNAEAFEWMKTLAPERPEADEIE
                     RKVVRLLREVKQASGGEGDEVAIARDIRLKLDAALYLFPARSEERTPDEPAPSSDWSV
                     YLHQRDVRGHAAVWGTLLSWLFVHELGRVAMAEDAAQRSRSAIDEWLFGRIIASALQD
                     AGLDEIAAWRAVTTVRILTDHQDWFADLTPEAGFAYRVVSDLLSDDEVRRFLQVNRHK
                     DVLWFNKESFEQLLWWLMATSAVTVHADSVRFDVDPAPATQACFDILRILQQAEETSE
                     YQVEKLLEAAKEEPSLI"
     gene            4646..5848
                     /gene="rocD"
                     /locus_tag="AC162G_17055"
     CDS             4646..5848
                     /gene="rocD"
                     /locus_tag="AC162G_17055"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - L-ornithine transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HIR5077569.1"
                     /translation="MKTQDYIAIEEQYGAHNYHPLDVVIERAEGVWVFDVEGKKYLDF
                     LAAYSAVNQGHCHPRLVKVLFEQASQVTLTSRAFRNTQLPLLEKELCELSGYQMMLPM
                     NSGTEAVETALKAARKWGYTVKHVEYGKAEIIACAGNFHGRTIAIVGMSTEPQYRDGF
                     GPYPAGFKVIPYGDADALAAAITRNTVAFLVEPIQGESGVNIPPDGYLKQVAGICKQN
                     NVLFMADEIQSGLGRAGKLFAHHWEGVRADVVIVGKALSGGMYPVSAVLSDREVLGVL
                     SPGDHGSTFGGNPLACAVAREALRVLIDENLFERSVELGEYFKGRLQRIESDAIKEVR
                     GRGLFIGVELKPEVGGARRFCEALKDRGLLCKETHENVIRFAPPLVVKKEELDWALEH
                     IEEVLTTL"
     gene            complement(5867..6964)
                     /locus_tag="AC162G_17060"
     CDS             complement(5867..6964)
                     /locus_tag="AC162G_17060"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR5077570.1"
                     /translation="MRRRDFLKLAGMALAGTALPQPLAALAAGGAPPEDQVADRFVPL
                     RRGRVLTSSVTVWDQIEAPHSPIKRYARDEVLLLGEERSVRGTGSAYNNLWYRTRGGW
                     VHSGWVQPMEFHARPAIYREAGPNGFWVEVIEPKTTTREGPSLSAPSIYQYVYGTVYL
                     VTDVAVEASGLVWYQTYDEYADQELGVAPTHHWVRADHVRRVPESEFMAIRPEVADKR
                     ILVDLDKQTLTCFEGDTPVVTTKVATGAAFVQADGSVVSYATPKGDHRAILKMPSRHM
                     RAIEGERNTDAWFDLPGVPWNTFFTYDGIAIHGTYWHNDFGVVRSHGCVNVPIPVAKF
                     IYRWTFPTAPYTDAFVRGDVKGMNATQIEVA"
     gene            complement(7103..7915)
                     /locus_tag="AC162G_17065"
     CDS             complement(7103..7915)
                     /locus_tag="AC162G_17065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5077571.1"
                     /translation="MTIAIRDLTTYEEFIQVREIQRQCWGFAQGEGLYPPVLNTAAHN
                     GGTVLGAFDEGRMIGFLFGFLGLHADRRLKLCSQTMGILPEYRNRGIAAALKWAQRER
                     VLALDIDLITWTYDPLEAPNARLNLHTLGAIAREYKRNVYGEDFGALGRGLPSDRFLV
                     EWWVKSEGVEQRAAGQEPEPVDLDSPIVNPCAGEGAARRIATVDLHADSPMLRVEVPA
                     DIQAVRQADMALAMEWRLKTRQIFETYFERDFCAVDLVRTDNGRNYYMLRQA"
     gene            8032..8619
                     /locus_tag="AC162G_17070"
     CDS             8032..8619
                     /locus_tag="AC162G_17070"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5077572.1"
                     /translation="MPHDADGRESILEVAAPLFAQKGYNGVSIRDIAQACGMTNAALY
                     YHFKNKEDLYLAVLRRGHEQGMASVAQAAGSTGDLRARLKQLVLRYGEVMCEQRQSFQ
                     TMRRDLAHLDDARAHKLLGEIRADFMRPIQQFIESGQSSGEIASGDARLWARLLHGMI
                     IAMTFEGRPGRPARVTPAQADAVVELFLEGAGARK"
     gene            8643..10544
                     /locus_tag="AC162G_17075"
     CDS             8643..10544
                     /locus_tag="AC162G_17075"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077573.1"
                     /translation="MKKRPSRRSGPPRPSMAGLRRALGYLGHYRKIMVIAYGSLLVAT
                     LAQLAVPTLVRNIIDAVTNGVIAGKILEAPQQFQSVILQQIGWTSDQLTYHATTAESI
                     LIVSGLAIIAFASVRGLFAFSQAYNAERLSQSMAFDLRNDLFAKVQRLSFSYHDRNQT
                     GQLMIRATDDVEKVRLFMGQGLIFSLQAIILLTGTLIILVLTNVRLTLVVLPVLPIAL
                     VLFMVFGAISQPLFGEVQRRLSKMNTILQENLAGIKVVKAFAREPQEQVKFDAAADSL
                     MRQAIKVTRVFSFLFPLVFLIANLGQAAALNAGGTQIIEGTLTLGEWQQFSLYLVFVF
                     FPLAQLGIIISQMAQASASADRIFEILDARSDVTDKPGAIALPPIQGRLEFKHVTFRY
                     PGGVTPVLSDVSFAAQPGQTIALLGATGSGKSSIINLIPRFYDPTGGAITIDGNDLRD
                     VTIDSLRRQIGIVLQETNLFSGTIRDNIAFGRTEATMDEVIDAAKAAAAHDFILEFPQ
                     GYDTPVGERGSTLSGGPKQRIAIARALLLDPRILILDDSTSSVDLVTEYRIQQALDRL
                     MDGRTSFVIAQRISTVLNADQILVLDNGQIVAQGVHEELMESSEIYAQIYQSQLSEDV
                     KLEEERVTA"
     gene            10621..>12145
                     /locus_tag="AC162G_17080"
     CDS             10621..>12145
                     /locus_tag="AC162G_17080"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077574.1"
                     /translation="MFGGPGGPRAIMEREVSKPKNTGETLRRFASYFRPYGLVLLLAA
                     GLVILSTWTQVTVPELIGQTVDCYLTPAASTGGGGFPGAAPALSRSPDPERSEGEGAA
                     EGAQEQSSSSSCWYDPSASALMSNEERITGLGSIVLRLVGLFVLGSVATGLTFYAMTW
                     AGNHVLKQLRVQVFEHLHRLSLGYYAENEAGNLMSRITNDTETIQQAVSFALVQVASG
                     LLLMTWITYNMFSKSVPMALISLAVVPLMWISTRWFSSQARKAFRKTRVVMGNVNAEL
                     QQSISSVREVQAFSREQENIDQFRDVNAANRDANIRAVAFTSALSPTLEALGYAALAL
                     VAGAGGLLLLRGQDLLGATISLGLVITFIAYVQRFTQPVHQIAVLWANIQSAIAGGER
                     IFGLLDVKPDVHDRPDAKAMPAIRGGVVFGDVHAEYEKDVPVLRGIDFKAEPGQTIAI
                     VGPTGAGKTTIINLIPRFYDVTGGAVTIDGVDVRDVTAASLRHQIGIVLQDSFLFSDT
                     "
BASE COUNT         2188 a         3807 c         3932 g         2218 t
ORIGIN      
        1 gctattcgca ccgcgacgac gagttcgtgc tgcgcctggc cacggacctg gaagaccgcg
       61 gtgcgtccgt ctggatcgat cagggcaaca tccagggcgg cgagcagtgg cgccggtcga
      121 tctctacggg tgtccaggac agcaaagctt tcgtgctggt tgtctcgccc gattcgatca
      181 actcggagta cgtggccgag gaactcgcgt cggccttcaa gcacgacagg ccgatcatcc
      241 cgctgcttta tcgcaagacg agtatcccgg caatgctgaa ggcgcaactg gacgcctacc
      301 agttcctcga ttttggcaag ggcggctatg cgcagaacct ggccgacctg gtcgccgcgc
      361 tggcacggca cggcgtggcg ctgcaagccg ccccggggga acaggcccgg cgacgccgcg
      421 agcggatggg cgcgcccgtg ccgacgaatt ggggagcggt tttcggccgc attccaggct
      481 gggccttcgg ctggggattg ggctgggcca tcttctggct ggtattgccg atcgtcatga
      541 ccgtcttgaa ccagtcggaa ctcgggaact ttccggcgct gccggtcggc ggcttcgccg
      601 gtggcatcgt cggcggactg ctggccggtc tgttcaccat gctcgcgctg cgacgcaacg
      661 ccggcagcat tcgctggaag catatgtcgc gctcgattcg catatggggg ctggtcgggc
      721 cgctgggtgt catcgccgcc ggtgcattgg ccgtgctgct gttcaatccc gatgccctcc
      781 tcggcaacag tccggagtgc tcccaggtgg gcttggccga gtgtttcatg cagggcatcg
      841 gctacgccat tggcgcgggt ctcgcggccg ggttggtcct ggtcttgacg atcatcttct
      901 acgccatgat cgcactgttc ggcgttggcc tgatcgccgg ctggctggcc gtgcgccaca
      961 tccgccggct ggagccgggc atcctgggcc ggcaggcgat ctgggtggtg ctgggatggg
     1021 gcggcggcgc ggtcctggcc gcgatcgccg gagtggctgt aatgtcattg ctgggggcat
     1081 agaatggaat tccacgtctc ccgacttgcc cgcgaccgtt accagttcga cgacatgctc
     1141 ttcgcctcga acggcaacgt cattttcgca aacttccatg ctgcgcgcac gttcgcgcaa
     1201 aagatgaacg cgaagcgcga cctggctcgc ttccccgaac aggcgatcaa ggccggccag
     1261 atcaacgcgc tggggctgat cgacgaaatc ctgcactatg tcgtcggcca gtatcgccag
     1321 cagatgagcc ccaacgtgat ggcgcaggcg ctggcggcgc tggacacgca gctaggtagg
     1381 gaagcggtcg atgcggcgct gcgccgattc gcagacgagt tcccggcgct ggctgtctat
     1441 cggcgcgaaa ttacgctcga cgcttatctc aacggtcaaa ccggcgacgt gcccaatcgg
     1501 cagatcatcc tcgaagaaat gctcatgctg tgggtcgcca acatgaaccc ggcctgcgcg
     1561 ccgttcatgg agctgttcga cgacaccgcg ctcgaaaaag aaaccaccta cctgcgcgtc
     1621 gtcgccggcc tgcgcgagtt tttcgataca caaccggtct tcggacccga caaccagaac
     1681 ctgatcgaca tgctgcgcag cccggctgtt gccgtgccgc actcgctgcc ggggcagctc
     1741 gaatacatcc aggcgcgttg gggtctcttg ttgggccggt atctatatcg cctgctgggt
     1801 agcctcgacc tgatcaggga ggaagagaaa ccgatctttg ccgggttcgg ccccggcgag
     1861 aagggtgtgc ccgtctacgt gttcgccgga ttggaaatcg aggccgagcg cttcacgccc
     1921 gatcgcgaat ggatgccgcg cctggtgctg atcgccaaga acgcctacgt gtggctcgat
     1981 caactgtcgc gccggtacca gcgcccgatc gcacgcctcg accagatccc cgatgaggaa
     2041 ctcgacacgc tggcgcgctg gggcatcacc ggcctgtggc tgatcggcgt gtgggagcgc
     2101 agcccggctt cgcagcgcat caagcaactc atgggtaacg cggatgcggt cgcgtcggcc
     2161 tactcgctgt atgcctacag cgtcgcgaac gatctcggcg gcgacgccgc gctgcagaat
     2221 ctcaaggagc gcgcgtggcg gcgcggcatc cgcctggcca gcgacatggt gcccaaccac
     2281 atgggcatcg actcgccgtg ggtggccgag catcccgact ggttcatttc gtcggacgcc
     2341 agcccgttcc cttcgtatgc cttcgacagc ccgaacctgt cgagcgacga gcgtatgggc
     2401 atctatctcg aagaccacta ctacagccgc tccgacgccg ccgttgtctt caagcgcgtc
     2461 gatcatgcga acggcagcgt gaagtacatc taccacggca acgacggcac gagcctgccg
     2521 tggaacgaca ccgcgcagtt gaattacctc aagccagaag tgcgcgaagc ggtcatccag
     2581 accatcttgc acgtcgcccg gcagttcccg atcatccgct tcgacgcggc gatgacgctg
     2641 gcgaagaagc actaccagcg cctgtggttt cccgagccgg gaaccggcgg cgcgatcccc
     2701 tcgcgcgccg acttcggcct gccccggcgc cagttcgaag cggcaatgcc ggtcgaattc
     2761 tggcgcgagg tcgtcgatcg ggcggccgtc gaagcgcccg acacgctgct gctcgccgag
     2821 gcgttctggc tgatggaggg ctacttcgtc cgcacgctcg gcatgcaccg cgtgtacaac
     2881 agcgccttca tgaacatgct gcgtgacgaa gacaacgcca aataccgctc ggtgatcaag
     2941 aacacgatcg aattcgatcc ggcgatcctc aagcgctatg tgaatttcat gaacaacccc
     3001 gacgagcgca cggcggtcga tcagttcggc aagagtgaca agtacttcgg cgtgtgcgtc
     3061 atgctggcga cgatgcccgg cctgccgatg ttcgggcacg gccagatcga gggcttggca
     3121 gagaagtacg gcatggaatt ccgccgtgcg ctgtgggacg agcagcccga ttccggcttc
     3181 gttcagcatc acgagcggct catcttcccg ctcttgcatc gtcgttatct gttcgcggac
     3241 gtcgagaact ttctattgta cgacctcttc acgccggaag gccacgtcga tgaagatgta
     3301 ttcgcctact cgaaccgccc ttcggggatc gcagggcaag cggagcgtgc gttggtggtg
     3361 taccacaata agttcggctc gacgcgtggc tggattcgca tgagcgccgc atacgctgtg
     3421 aaaaccggcg agggtggtga ccgtgtgctc gcgcagcgca cgctggccga agggctggac
     3481 atccgcgacg aggccggctt ttacaccatc ttccgcgatc acgtcaccgg cctggaatac
     3541 ctccgcagca atcacgatct ggccgagagc gggttgtaca tcgagctggg cgcgtacgag
     3601 caccacgtct tcctcgactt tcgcgaggtg cgcgacgacg agcggcgcca gtacgcgcag
     3661 ttgcacgcgt atctcggcgg gcgcggcgtg cccagcgtcg acgaggcgat gcgcgagctg
     3721 ttcctgcagc cgattcaccg tgccttcgcg gacctggtca acgcggaggc tttcgagtgg
     3781 atgaagacac tcgcaccgga gcgccctgag gcggacgaga tcgagcggaa agtcgtgcgg
     3841 ctgcttcgcg aagttaagca ggcttccggc ggcgaaggcg acgaggtcgc cattgcgcgc
     3901 gacattcggc tcaaactgga cgctgctctg tatctgttcc ccgcgcgctc cgaagagcgg
     3961 acgcctgatg agccggcacc ttcttccgac tggagcgtct acctgcatca gagggatgtg
     4021 cgggggcatg cggctgtctg gggcacgctg ctgagctggc tgttcgttca cgagttggga
     4081 cgggtcgcca tggccgaaga tgcggcgcag cgcagccgca gcgcgatcga cgagtggctg
     4141 ttcggacgga tcatcgcctc cgcgctgcaa gacgccgggc tggacgaaat cgccgcctgg
     4201 cgcgcggtca cgaccgtgcg catcctgacg gatcaccagg attggtttgc cgacctcacg
     4261 ccggaagccg gtttcgccta tcgtgtcgtc tcggacctgt tgagcgacga cgaggtccgc
     4321 cgcttcctcc aggtgaaccg tcacaaggac gtcctgtggt tcaacaagga atccttcgag
     4381 cagttgctgt ggtggctgat ggcgacgtcg gccgtgacgg tccacgccga tagcgttcga
     4441 ttcgacgtcg atccggcgcc ggcgacccaa gcgtgcttcg acatcctgcg catcttgcag
     4501 caggccgaag agacatccga gtatcaggtc gagaagctac tcgaggccgc caaagaagag
     4561 ccgagcctga tataattcga ccctgatgtc gctcaagttc gttgagttcc tcgcaaggga
     4621 actgagggaa ctctaaggat accggatgaa aacgcaagac tatatcgcca tcgaagagca
     4681 atacggtgcg cacaactatc acccgctcga cgtcgtgatc gagcgcgccg agggcgtatg
     4741 ggtgttcgac gtcgagggca agaagtacct cgattttctt gcggcgtact cggcggtcaa
     4801 ccagggccac tgccatccgc gcctcgtcaa ggtgctgttc gagcaggcga gccaggtgac
     4861 gctgacttcg cgcgccttcc gcaacacgca actgccgctg ctcgaaaagg agctgtgcga
     4921 gctgagcggc tatcagatga tgctgcctat gaactctggg acggaagctg tcgagacggc
     4981 gctcaaggcc gcacgaaaat ggggctacac tgtgaaacat gtcgagtacg gcaaggccga
     5041 gatcatcgcc tgcgccggca acttccacgg ccggacgatc gccatcgtcg gcatgagcac
     5101 cgagccgcag tatcgcgacg gcttcggccc ttacccggca ggcttcaagg tcatccccta
     5161 cggcgacgcc gacgcgctgg ccgcggcgat cacgcgcaac acggtcgcgt ttctggtcga
     5221 gccgatccag ggtgagagcg gcgtgaacat tccgcccgat ggctacctca agcaggtcgc
     5281 cggcatctgc aagcagaaca acgtgttatt catggccgac gagattcaga gcgggctggg
     5341 ccgcgcgggg aagctcttcg cgcatcattg ggagggcgtg cgcgccgacg tggtgatcgt
     5401 tggcaaggcg ttgtccggtg ggatgtaccc tgtctcggcg gtgctgtccg atcgcgaagt
     5461 cctcggcgtt ctcagtccag gcgaccacgg cagcaccttc ggcggcaacc cgctcgcctg
     5521 cgccgtcgcg cgcgaggcgc tgcgcgtcct gatcgacgaa aacctgttcg agcgctccgt
     5581 cgagctgggc gagtacttca agggacgcct gcagcgaatc gagagcgatg ccatcaaaga
     5641 ggtgcgcggg cgcgggctgt tcatcggtgt cgagctgaaa ccggaggtgg gcggcgcgcg
     5701 gcgtttctgc gaagccttga aggatcgcgg tctgttatgc aaggagacgc acgagaacgt
     5761 catccgcttc gccccgccgc tcgtcgtgaa gaaagaggag cttgactggg cgctggagca
     5821 catcgaagag gtgttgacga cgctgtaaaa gaaacctggt ttccgatcag gctacttcga
     5881 tctgcgtagc gttcattccc ttcacgtcgc cgcgcacgaa ggcgtcggtg tacggggcgg
     5941 tggggaacgt ccagcgatag atgaatttgg cgaccgggat cggcacgttg acgcagccgt
     6001 ggctgcgcac cacgccgaag tcgttgtgcc agtacgtccc gtggatggcg atgccatcgt
     6061 acgtgaagaa ggtgttccat ggcacgccgg gcaggtcgaa ccaggcgtcg gtgttgcgct
     6121 cgccttcgat cgcgcgcatg tggcgggacg gcatcttcag gatcgcgcga tggtcgccct
     6181 tcggcgtggc gtagctgacc acactgccgt cggcctgcac gaatgcagcc ccggtggcga
     6241 ccttggtggt taccacaggc gtatcgccct cgaagcaggt gagcgtctgc ttatcgagat
     6301 cgacaaggat gcgcttgtcg gcgacttcgg gccggatggc catgaactcc gactcaggta
     6361 cacgccggac gtggtcggcg cgcacccagt gatgtgtcgg tgcgacgcct agctcctggt
     6421 ccgcatactc gtcgtacgtc tggtaccaca ccaacccgct tgcctcgacg gccacatccg
     6481 tgacgagata gaccgtgccg tagacgtact ggtagatcga cggagcggac agcgatggcc
     6541 cttcgcgcgt cgtcgtcttc ggctcgatga cttcgaccca aaagccgttc ggaccggcct
     6601 cgcgatagat ggctggccgc gcatggaatt ccatcggctg cacccagccc gagtgcaccc
     6661 agccgccgcg cgtgcggtac cacaggttgt tgtacgcgct gcctgtgcct cgcacgctgc
     6721 gctcctcccc aagcagcaga acctcgtcgc gtgcgtaccg cttgatgggg gagtgcggcg
     6781 cttcgatctg atcccagaca gtcaccgagg acgtcagcac gcgtccgcgc cgcagcggga
     6841 cgaatcgatc ggccacctgg tcttccgggg gcgctccgcc cgcagccagc gcagccagtg
     6901 gctggggcag ggcggtgccc gccagcgcca tgcctgcaag cttgaggaaa tcgcgtcgtc
     6961 tcatcggctt tctcaaccga gcgaattata tcgatcctcg gcctggaaat caacctgtcc
     7021 tgggtaacgg tgtgcacgca aaacatgtca ggcaggatat gggtaacacg tgcgcttaga
     7081 ggcggaaacc ttgatgcagt tttcacgcct gcctgagcat gtagtaattt cgcccgttgt
     7141 ccgtccttac caagtctact gcgcagaaat cgcgctcgaa atacgtctcg aatatctgcc
     7201 gcgtcttgag ccgccattcc atcgccagtg ccatatccgc ctgcctgacg gcctgaatgt
     7261 cggcggggac ctcgacccgc agcatggggc tatcggcgtg cagatccact gtggcaatgc
     7321 gccgtgccgc gccctcgccg gcgcacggat tgacgatcgg gctgtcgaga tcgaccggtt
     7381 ccggctcctg cccggccgca cgctgctcga ctccctcgct cttcacccac cattcgacga
     7441 ggaagcgatc gctcggcagg ccgcgcccca acgcgccgaa gtcctcgccg tagacattgc
     7501 gcttatactc gcgcgcgatc gcgccgagag tgtgcaggtt caggcgggcg ttcggcgctt
     7561 cgagcggatc gtaggtccac gtgatcaggt cgatgtccag ggccagcacg cgctcgcgct
     7621 gcgcccattt tagcgcggcg gcgatgcccc ggttgcggta ctccggcaaa atgcccattg
     7681 tctgcgagca gagcttgagc cggcgatccg cgtgcaggcc caggaacccg aacaggaacc
     7741 cgatcatccg gccctcgtcg aatgcgccga gcaccgtgcc gccgttgtgc gcggcggtgt
     7801 tcagcacggg cgggtacagc ccctcgccct gtgcgaaccc ccagcactgc cgctgaatct
     7861 cgcgaacctg aatgaactcc tcgtacgtcg tgaggtcacg aatggcaatg gtcatcgtgt
     7921 gtgcattata aacgaggcgg acacccctgt ccatgtggac aggttgacaa tcgcgcgatt
     7981 catgatatgc tggtcgtcat tactgaacgt ttggtaagta cggagtgagc catgccgcac
     8041 gacgccgatg gacgcgagtc gattctggaa gtggccgccc ccctgttcgc ccagaagggc
     8101 tacaacggcg tgtcgatccg cgacattgcc caggcgtgcg gcatgaccaa cgcggcgctg
     8161 tactatcact tcaagaacaa agaagatctg tacctggccg tgctgcgccg cggtcacgag
     8221 caagggatgg cgtccgtcgc ccaggcagcc ggctcgacgg gcgatctacg ggcgcgtctc
     8281 aagcagttgg tgctgcgcta cggcgaagtg atgtgcgagc agcgccagtc gttccagacg
     8341 atgcggcgcg acctggcgca cctcgacgac gcgcgcgcac acaagctgct cggcgaaatc
     8401 cgcgccgatt tcatgcggcc catccagcag tttatcgagt ccgggcagtc aagcggcgag
     8461 atcgcctcag gcgacgcgcg gctgtgggcg cgcctgctgc acggcatgat catcgccatg
     8521 accttcgaag gccggccggg ccggccggcg cgggtgaccc cggcgcaggc ggacgcggtc
     8581 gtcgagctgt ttctggaggg ggccggcgct cgcaaatgat cttgagcaga aggggaaaca
     8641 acatgaagaa acgtccatcc aggagatccg gcccgccgcg cccgagcatg gcaggtttgc
     8701 ggcgggcgct tggatacctg ggccattacc gcaaaatcat ggtgattgcc tacggcagcc
     8761 tgctggtcgc gacgctggcg cagctcgccg tgccgacgtt ggtgcgcaac atcatcgacg
     8821 ccgtgaccaa cggcgtgatc gccggcaaga ttctcgaagc gccgcagcag ttccaatcgg
     8881 tgattttgca gcaaatcggc tggacgtcgg atcaattgac ctaccacgcc accaccgcgg
     8941 agtcgatcct gatcgtgtcc ggactggcga tcatcgcctt cgccagcgtg cgtggtcttt
     9001 tcgccttctc gcaggcttac aacgccgagc ggctgtcgca gagcatggcc ttcgacctgc
     9061 gcaacgatct gttcgccaaa gtgcagcgcc tgtcgttcag ctaccacgat cgcaaccaga
     9121 ccggccagtt gatgattcgc gccaccgacg acgtggagaa agtgcggctg ttcatggggc
     9181 agggcctgat cttctcgctg caggctatca tcctgctgac cggcacgctg atcattttgg
     9241 tgctgactaa cgtgcgattg acgctggtcg tgctgcccgt cctgccgatt gcgctggtgc
     9301 tgttcatggt cttcggcgcg atcagccagc cgttgttcgg cgaagtgcag cgccgcctgt
     9361 cgaagatgaa taccatcctg caggaaaacc tcgccggcat caaggtcgtc aaagccttcg
     9421 cgcgcgaacc gcaggagcag gtcaagtttg acgcagccgc cgattcgttg atgcggcagg
     9481 cgatcaaggt gacgcgcgtg ttctcgtttc tgttcccgct cgttttcctg atcgccaacc
     9541 tgggccaggc cgcggcactg aatgccggtg gcacgcaaat tatcgaaggc acgctgacgc
     9601 tgggcgagtg gcagcagttc agcctgtacc tggtgtttgt gttcttcccg ctcgcccagc
     9661 tcggcatcat catttcgcag atggctcagg ccagcgcgtc ggccgatcgc atcttcgaga
     9721 tcctcgacgc caggagcgac gtgacggaca agcccggcgc gatcgcgctg ccgccgattc
     9781 agggtcgcct cgaattcaag cacgtcacct tccgctatcc cggcggcgtc acgcccgtct
     9841 tgagcgatgt gagtttcgcc gcacagcctg gccagaccat cgcgctgctc ggggcgacgg
     9901 gcagcggcaa gagctcgatc atcaacctga ttccgcgctt ctacgacccg acgggaggcg
     9961 cgatcacgat cgacgggaac gacttgcgcg acgtgacgat cgattcgctg cgaagacaga
    10021 tcggcatcgt gctgcaggag accaatctgt tcagcggcac gatccgcgac aacatcgcgt
    10081 ttggccgcac cgaggcgacg atggacgagg tgatcgacgc ggcgaaggcg gcggcggcgc
    10141 acgacttcat cctggagttc ccgcagggct acgacacgcc ggtcggcgag cgcggatcga
    10201 cgctcagcgg cgggccgaag cagcgtatcg ccatcgcgcg cgccctgctg ctcgatccgc
    10261 gcatcctgat cctcgacgac tcgacgagca gcgtcgacct agtgacggag tacaggattc
    10321 agcaggcgct cgatcggctg atggacggcc gcaccagctt cgtcatcgcg cagcgcatca
    10381 gcacggtgct caacgccgat cagattctgg tgctcgacaa cggccagatc gtcgcccagg
    10441 gcgtgcacga ggagttgatg gagtcaagcg agatttacgc gcaaatctat cagtcgcagc
    10501 tcagcgagga tgtgaagctc gaagaagagc gggtgacggc ataaagtggg acaacgttgc
    10561 gtgatcatgt aattacgaat taccaattac catttgcgtt gtacgcatca ggagagactc
    10621 atgttcggtg gacccggcgg accacgcgcc atcatggagc gcgaggtctc caaacctaaa
    10681 aataccggcg aaacgctcag gcgctttgca tcctacttcc gcccctacgg gttggtgctg
    10741 ctgcttgcag ccgggctggt gatcttgtcc acgtggacac aggtcactgt gccggagctc
    10801 atcgggcaga cggtggactg ctaccttacg ccggcggcgt cgaccggcgg cggaggcttc
    10861 cccggcgcag cgcctgccct cagccgaagc cctgaccctg aacgaagtga aggggaaggg
    10921 gcagccgaag gggcgcaaga gcaatcgtct tcgagctcgt gctggtacga tccgtcggcc
    10981 tcggcgctga tgtcgaacga agaacgcatc acggggctgg gcagcatcgt gctgcgattg
    11041 gtcgggttat tcgtgctcgg ctcggtcgcc acggggctga ccttttacgc gatgacgtgg
    11101 gcgggtaatc acgtgctcaa gcaactgcgc gtgcaagtct tcgagcactt gcaccgcctg
    11161 tcgctcggct actacgcgga gaatgaggcc ggcaacctga tgagccgcat cacgaacgac
    11221 acggagacga tccagcaggc ggtcagcttc gcgctggtgc aggtcgccag cggcctgctg
    11281 ctgatgacat ggatcaccta caacatgttc agcaagagcg tgccgatggc gctgatcagc
    11341 ctggccgtcg tgccgttaat gtggatctcg acgaggtggt tctcgtcgca ggcgcgcaag
    11401 gcgttccgca aaacgcgcgt agtgatgggc aacgtcaacg ccgagctgca gcagagcatc
    11461 tccagcgtgc gcgaggtgca ggccttcagc cgcgagcagg agaatatcga tcagttccgc
    11521 gacgtgaatg cggccaatcg cgacgccaat atccgcgcgg tcgcgttcac ctcggcgctg
    11581 tcgcccacgc tggaggcgct gggttatgcg gcgctcgcgc tggtcgcagg cgcgggcggc
    11641 ctgctgctgc tgcgcggaca ggatctgctc ggcgcgacga tctcgctcgg actggtgatc
    11701 accttcatcg cctacgtgca gcgcttcacg cagccggtgc atcagatcgc cgtattgtgg
    11761 gcgaacatcc agagcgcgat cgcgggcggc gagcgcatct tcggcctgct cgacgtgaag
    11821 cccgacgtgc acgaccggcc cgacgcgaag gcgatgcccg cgatccgggg cggcgtggtg
    11881 ttcggcgatg ttcacgctga gtatgagaaa gacgtgccgg tgctgcgcgg catcgacttc
    11941 aaggctgagc cgggccagac catcgccatc gtcggcccga ccggcgcggg caagacgacg
    12001 atcatcaacc tcatcccacg cttctacgac gtgaccggcg gcgcggtgac gatcgacggc
    12061 gtcgacgtgc gcgacgtgac ggcggccagc ctgcgccacc agatcggcat cgtgctgcag
    12121 gacagtttct tgttcagcga cacgg
//
LOCUS       DBNHXB010000434         5440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4661139, whole genome shotgun sequence.
ACCESSION   DBNHXB010000434 DBNHXB010000000
VERSION     DBNHXB010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4661139"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1311
                     /locus_tag="AC162G_17085"
     CDS             100..1311
                     /locus_tag="AC162G_17085"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR5077575.1"
                     /translation="MRAIYVDRKLPRMAAVKALRGVWPGVCWSPLSPATVTHLPDPPL
                     PGPRWIRVKNHRCGVCATDVSLLLVRVDPSVGPAALPAYNRFFLGHEVVGTVVEVGSG
                     AARVKPGERVIMDTRFHGANCLSQEIEPPCRFCARGERNLCENVAANVGPRGVGGGWG
                     DGYTAHETEVYPIPDDISDDVATLVEPMAVGVHAVLRRPPNPGDKVLVLGVGIIGLLT
                     LQAARAACPDCYLTALAKYAHQAEVARRLGADEVIMGSDGYAAVARATGAKHYTAWMN
                     KGMLLGGFDVIYDCVGSGATIEDSLRWARAGGAAVLVGIDLERVKLDLNSIWYQEVDL
                     IGSKGHGMDGWRGERRHSYDWVLRWIREGKLTGDGLITHRFRFEDYKRAVAASLDKRG
                     AKSIKVIFEYR"
     gene            1324..2649
                     /locus_tag="AC162G_17090"
     CDS             1324..2649
                     /locus_tag="AC162G_17090"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5077576.1"
                     /translation="MSNGSTVIGKQADVVVVGSGPGGATVVRELSRAGRKVILLERGK
                     DYRKWALYGTYLGATTYTDRHALLFTREGLNIIRPLMAGGATSMYCGCATRPPAWLKD
                     KYGIDIERRVDEAIAELRIAPLPPELRGIASTRIAEAANALGYDWEPLPKFMDPSRAR
                     RFDCGAKCMLGCRCGAKWNAAEWVDEAVAAGCEFLTEARVDDVLVENKKAVGVRGRLK
                     GRPFEIRGRAVVLAAGGIGSPLLLQRTGLHRAGQGIAMDTTLIMYGASRFPGNGKEPP
                     MTYAWADDEMGYMLSTLVDPWLMYPVITALKGFRYPLTWPRWGNTLGIMIKLKDDVSG
                     SLESERRISKPFTPSDQRKLDHATEMARRILIKVGAEPESIFITPMRGTHPCATVRIG
                     HLLDRDLQTEIENLYVCDASAFPEALDRPTVLTIIGLGKRLSERLAYRL"
     gene            2805..2927
                     /locus_tag="AC162G_17095"
     CDS             2805..2927
                     /locus_tag="AC162G_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077577.1"
                     /translation="MQEDDVLIARPLALTDQIDQSGHGFRRINGIEQQAFLCGD"
     gene            complement(2924..3781)
                     /locus_tag="AC162G_17100"
     CDS             complement(2924..3781)
                     /locus_tag="AC162G_17100"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate
                     deaminase/D-cysteine desulfhydrase"
                     /protein_id="HIR5077578.1"
                     /translation="MSFDTKLAEYPRILIGQFPTPLEPLPRLTQVLGGPHLWVKRDDT
                     VGPAMGGSKTRQLEFLFGEAQAKRAQIVATFGGLQSNFARQMCAVARSLGVEPHCFYF
                     ARRPTRLEGNLLLAQLLGAHLHFIPFGGGSSATMTPEQAARLVRWIVRLTPACWGRRV
                     YFMPVGGHTPLGCLGYVLAAMEIENQLRAHGIERATIVTASGTGATLAGLLAGFHLLR
                     SRHALLGTTSASCGARSRLPSQRLRRRPAHCWANPITFRPGTCPSSREFTSEKGTHSS
                     RRRRSRQVG"
     gene            complement(3796..4851)
                     /locus_tag="AC162G_17105"
     CDS             complement(3796..4851)
                     /locus_tag="AC162G_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 periplasmic-binding domain-containing
                     protein"
                     /protein_id="HIR5077579.1"
                     /translation="MLNKSILAACLAFLIAACATPAPETAAPAPASIELQPTSTPIPP
                     TPTPSATPEPSATPVPRREIVLERGNVICGIDVARPDLAGFDVDFCRAVAAALFDDAE
                     AIDVRPLDLASGAAALRSSAIDVFIGGPAHTGVPEGPFGSQDWFFDAAGAIARTDVGI
                     TRLADLKFATVCMIQGSIEERLFNEAAGAARVRVQPYLFNPGDDDAMYRTYDEGRCDA
                     VVDNRVRLAQRLPALSAPREHALIDLTLPIGWHNPLTDLSDAQWSEAIASIGYSLFRA
                     EELGVSSENVDTALSSDESAVQELLLGSRLGFSSDFVARVIRLVGNYGEVYARHFPDL
                     PRGPNALATDGGLIGVP"
     gene            complement(5087..>5440)
                     /locus_tag="AC162G_17110"
     CDS             complement(5087..>5440)
                     /locus_tag="AC162G_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR5077580.1"
                     /translation="IPEQAHKYPGQLSGGQQQRVAIARALAMDPKIMLFDEPTSALDP
                     EMIKEVLDVMVELAHSGMTIVAVTHEMSFARSVAHRMVFFDEGVIVEQGTPHQIFNDP
                     QHDRTKLFLSQILKH"
BASE COUNT          995 a         1651 c         1829 g          965 t
ORIGIN      
        1 gaaaactggg gtgcaggctt cagcctgccg catctgaagc ggactgaagt ccgcactccg
       61 gtgattttcg gataggctct tggttggctt ggagtgctca tgcgcgcgat ctacgtcgat
      121 cgtaagctgc ccagaatggc tgccgtcaag gcgctcagag gagtgtggcc cggcgtgtgc
      181 tggtcgcccc tcagcccggc cacggtcacg cacctgcccg atccgccgct gcccggcccg
      241 cgttggatca gggtgaagaa tcatcgatgc ggcgtgtgcg ccacggacgt gtcgctgctt
      301 ctcgtgaggg tcgatccctc agtcgggccg gctgcgctgc cggcatacaa tcgtttcttc
      361 cttggacacg aggtggtcgg taccgtcgtc gaggtgggtt cgggcgcggc gcgggtcaag
      421 ccgggagaga gagtgattat ggacacgcgc ttccacggcg cgaactgtct gagccaggag
      481 atcgaaccac cctgccggtt ctgcgcgcgt ggcgagcgga atctgtgcga gaacgtggcg
      541 gccaacgtcg ggccgcgagg cgttggaggc gggtggggcg acggctatac ggcgcacgag
      601 accgaggtct accccatccc tgacgatatc tctgacgatg tggcgacact cgtcgagccg
      661 atggcggtgg gggtgcacgc cgtcctgcgc cggccgccga atcccggcga caaggttctc
      721 gtgctgggcg tgggcatcat cggcctgctc accctgcagg cggcgcgggc cgcatgcccg
      781 gactgctacc tgacagcgct ggcgaaatac gcgcaccagg ccgaggtggc gcgccgcctg
      841 ggcgcagacg aagtcatcat gggatcggat ggatacgcgg ctgtcgcgcg cgccaccggg
      901 gcgaagcatt atacggcatg gatgaacaag ggtatgctgc tcggcgggtt cgacgtgatt
      961 tacgattgtg tggggagcgg agcgacgatc gaagacagcc tgcgctgggc gcgggccggc
     1021 ggcgccgccg tcctcgtcgg catcgacctc gagcgcgtga aactcgacct gaattcgatc
     1081 tggtatcagg aagtcgatct gatcggctcc aagggccacg ggatggacgg gtggcgggga
     1141 gagcggcgac attcctacga ctgggtcctg cgctggattc gcgaaggcaa gttgacgggt
     1201 gatggcctga tcacgcatcg cttccggttc gaggactaca agcgcgccgt cgcggcgagc
     1261 ctggacaagc gaggcgcgaa gtctatcaaa gtgatctttg agtaccgtta gcaacgaggt
     1321 acgatgtcga acgggtcgac ggtcatcggc aagcaagcgg atgtagtcgt ggtcggctca
     1381 ggccctggcg gcgcgacggt agtccgcgag ttgtcacgcg cgggccggaa agtcattctt
     1441 ctggagcgcg gcaaggacta ccgaaaatgg gcgctctacg gcacgtacct cggcgcgacg
     1501 acttacacgg atcgtcacgc gctgctgttc acccgtgaag gcctgaacat tattcgcccg
     1561 ctcatggccg gcggcgcaac gagcatgtac tgtggatgtg cgacccgccc gcctgcctgg
     1621 ctgaaagata agtatgggat cgacatcgag cggcgtgtag atgaagctat cgccgaactc
     1681 cgcattgcgc cgctgccgcc tgaactgcgc ggtattgcct caacgcggat cgccgaggct
     1741 gccaacgctc tcggatatga ctgggagccg ctgccgaaat tcatggaccc atcgcgtgcc
     1801 cgccggttcg actgcggcgc gaagtgcatg ctcggttgtc gttgcggcgc caaatggaac
     1861 gcagcggagt gggtggatga agccgtcgcc gccgggtgtg aattcttgac cgaagcgcgc
     1921 gtcgacgatg tcctcgtcga gaataagaag gccgtcggcg tgcgcgggcg cctgaagggc
     1981 aggccgttcg agatacgggg ccgcgccgtg gtgctggccg ccggcggcat cggttcgcca
     2041 ctgctccttc agcgcaccgg gctgcaccgg gcagggcaag gcatcgcgat ggatacgacg
     2101 ttgatcatgt acggcgcatc acggtttcct ggcaacggaa aagagccacc gatgacttac
     2161 gcgtgggccg acgacgagat gggctacatg ctcagcacgc tcgtcgatcc gtggctgatg
     2221 tacccggtga tcaccgcact gaagggcttc cgctacccgc tcacgtggcc gcgctggggc
     2281 aatacgctgg ggatcatgat caagcttaaa gatgacgtct ctggcagcct ggagtccgag
     2341 cgccgcatta gcaaaccctt cactccctcg gaccaacgca agctagacca cgcgaccgag
     2401 atggcacggc gcatcttgat caaggtcggc gcggaaccgg aatcgatctt catcacgccc
     2461 atgcgtggca cgcatccgtg tgccaccgta cgcatcggcc acctgctcga tcgggatctt
     2521 cagacggaga tcgagaacct ttacgtgtgt gacgcgagcg cattccccga ggcgctcgat
     2581 cgcccgaccg tgttgacgat catcggcctg ggtaagcgat tgagcgagcg tttagcttat
     2641 cgcctatagc gtatcgcgta tcgttaatca cccatggcgc atggatcacc tgagaaaaga
     2701 gattagagat tggcgataag ccatcagcga taagcgattt gctatcagcg atatgcgata
     2761 agcgattcgc cgtggtacgc ccacaatgcc ggagcaccac ccgtgtgcag gaagacgacg
     2821 tgttgatcgc gcgcccactg gccttgacgg accagatcga tcagtccggc catggctttc
     2881 gacgtataaa cgggatcgag cagcaggcct tcctgtgcgg cgactagccg acctgccgcg
     2941 agcgacgcct gcgtgagctg tgcgtaccct tctccgacgt aaactccctc gacgatgggc
     3001 acgtccctgg cctgaaagtg atggggttcg cccagcagtg tgcaggtctc cgacgcaagc
     3061 gctgcgatgg aagccgcgaa cgcgcaccac agcttgccga cgtcgtgccc agcagggcat
     3121 gcctgctccg gagcaggtgg aaacctgcga gcaggcccgc cagcgttgct ccggttcccg
     3181 acgcggtgac gatggtagca cgctcgatcc catgtgcgcg cagttggttc tcgatttcca
     3241 tggctgccag gacgtatccc aggcacccaa gcggcgtgtg ccccccgacg ggcatgaagt
     3301 acacgcggcg tccccaacag gccggcgtca gccggacgat ccaccttacc aggcgcgctg
     3361 cctgttccgg agtcatcgtc gcgctgctgc ccccgccgaa cgggatgaag tgaagatgcg
     3421 cccccagcag ttgagcgagc agcaggttgc cctcgagtct cgtcggtcgc cgcgcaaagt
     3481 agaagcagtg cggttccacg ccgagcgatc tcgctaccgc gcacatctgc cgggcgaaat
     3541 tggattgtag cccgccaaac gtcgcgacga tttgcgcgcg ctttgcctgc gcttcgccga
     3601 agaggaattc gagctggcgc gtcttgctgc cgcccatggc cgggccgact gtatcgtcac
     3661 gcttaaccca aagatgagga ccgcccagca cctgggtgag tcgaggtagt ggctcgagcg
     3721 gcgtcggaaa ctggccgatc aggatgcggg gatattctgc gagtttcgtg tcgaacgaca
     3781 tggcaggatc gcaggttacg gcacgccgat caggccaccg tctgtcgcca gcgcgttcgg
     3841 gccgcgcgga aggtcgggga agtgccgcgc gtagacctcg ccgtagttgc cgacgaggcg
     3901 gatgacacgc gcgacgaagt cgctgctgaa tcccaggcgg gagccaagaa gcagctcctg
     3961 cacggcggac tcgtcgctac tgagcgccgt gtcaacgttc tctgaactga cgccgagttc
     4021 ctcggcgcgg aacaggctgt aaccgattga cgcgatggcc tcgctccatt gggcgtcgct
     4081 taagtccgtc agtggattgt gccagccgat cgggagcgtc aggtcgatca acgcgtgttc
     4141 gcgcggcgcg ctgagggcgg gcagccgctg cgccagtcgc acgcgattgt cgacgacggc
     4201 atcacagcgc ccttcgtcat aggttctgta catcgcgtcg tcgtcgcctg gattaaacaa
     4261 atacggctgc acccgcacgc gcgcggctcc ggctgcctcg ttgaagaggc gttcctcaat
     4321 cgagccttga atcatgcaca cggttgcgaa cttcagatcg gcgagtcgcg tgatgcccac
     4381 atcagtacgg gcgatcgcac ctgcggcgtc aaagaaccag tcctgggagc cgaaggggcc
     4441 ttccggcacg ccggtgtgcg ccggcccgcc gatgaagacg tcgatggcgc tggatctcag
     4501 cgcggccgcg ccggacgcca gatcgagcgg gcggacgtcg atcgcctcgg catcgtcgaa
     4561 cagggcggcg gcgacggcgc ggcagaagtc aacgtcgaag ccggctaggt caggccgggc
     4621 cacgtcgatg ccgcagatca cgttgccacg ctcgaggacg atctcccgtc ttggcacggg
     4681 cgtggccgac ggttccggag tcgcgctggg cgtcggcgtc ggtggaatgg gagtcgacgt
     4741 cggctgcaac tcgatcgacg cgggcgccgg cgcggcagtc tcgggtgcag gcgtcgcgca
     4801 agcggcgatc aggaatgcga ggcaggcagc cagaatcgat ttatttagca cgcagcgctc
     4861 ccgagaaatc gtcagacctg ccaggtctcg aagacctggc aggtctgatt ataccggatg
     4921 cgtcgaaact atggcagatt agcgcaagca aacgaccggg tctccccagc ggggatagac
     4981 ttcggaagtc tcaaggcgta actcggcagg cgagggagtc ggcctggcag gctcgtctcg
     5041 caggcgaggc tactcctgaa gacttccgaa gtcttcacta atcacgctag tgtttcagga
     5101 tctgcgagag gaacagcttg gtacgatcgt gctgcgggtc gttgaagatt tgatggggtg
     5161 tgccctgctc gacgatcacg ccctcgtcga agaagaccat gcgatgagcc acgctgcgcg
     5221 cgaagctcat ctcgtgcgtc accgcgacaa tggtcatgcc ggagtgggcc agctcgacca
     5281 tcacgtcgag cacttccttg atcatctccg ggtcgagcgc cgaggtcggc tcgtcgaaca
     5341 gcatgatctt ggggtccatc gccagcgcgc gggcgatcgc tacgcgctgc tgctgcccgc
     5401 ccgacaactg gccgggatac ttgtgcgcct gctcggggat
//
LOCUS       DBNHXB010000435         6795 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2986151, whole genome shotgun sequence.
ACCESSION   DBNHXB010000435 DBNHXB010000000
VERSION     DBNHXB010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6795)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2986151"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="AC162G_17115"
     CDS             complement(<1..375)
                     /locus_tag="AC162G_17115"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR5077581.1"
                     /translation="MTAADHKTLIEALFSGDDDRAEEAALALGRLGDAALPVLRDLLS
                     HPDGDCRWWVTRALGEIDSAAAIALLIDRCHDVDPDVRACAIYALGALDGEAASAIPA
                     LIGRLADPSVYVGQLAADSLARI"
     gene            complement(434..667)
                     /locus_tag="AC162G_17120"
     CDS             complement(434..667)
                     /locus_tag="AC162G_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR5077582.1"
                     /translation="MVSTDLLEILRCPHCVSGPTRREGPDPGRLVLVKGVWLVCQEPG
                     CDRKYPIQDDIPIMLIEEGDKWRNVPVEQLGQP"
     gene            790..879
                     /locus_tag="AC162G_17125"
     tRNA            790..879
                     /locus_tag="AC162G_17125"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:824..826,aa:Ser,seq:tga)
     gene            895..983
                     /locus_tag="AC162G_17130"
     tRNA            895..983
                     /locus_tag="AC162G_17130"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:929..931,aa:Ser,seq:gct)
     gene            1016..1087
                     /locus_tag="AC162G_17135"
     tRNA            1016..1087
                     /locus_tag="AC162G_17135"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1049..1051,aa:Arg,seq:gcg)
     gene            1250..1543
                     /locus_tag="AC162G_17140"
     CDS             1250..1543
                     /locus_tag="AC162G_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077583.1"
                     /translation="MRKRRWLTIVVAGILAFCLLGWALAYYFVGVDVSFIAGMVRARM
                     ADGRQAMPVAPAGVEVTTVADELDAPTSLAWSPDGAIYVADFGAGKVYRFAVP"
     gene            1730..1969
                     /locus_tag="AC162G_17145"
     CDS             1730..1969
                     /locus_tag="AC162G_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR5077584.1"
                     /translation="MNWKERIVVDPSILVGKPVVKGTRLAVEFIVDLLAQGWSEADLL
                     RNYPGLTHEDIQACLSYASAILKAEKVYPFEMSQA"
     gene            1973..2326
                     /locus_tag="AC162G_17150"
     CDS             1973..2326
                     /locus_tag="AC162G_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HIR5077585.1"
                     /translation="MRILANENFPGDAVNALRNQGHDVAWVRTDAPGTSDVDVLAHAQ
                     RENRIVVTFDKDFGELAFRARLPASSGIILFRISAPSPAHVARVAVAALESRTDWAGH
                     FTVVEDRRIRMTPLP"
     gene            complement(2450..4696)
                     /locus_tag="AC162G_17155"
     CDS             complement(2450..4696)
                     /locus_tag="AC162G_17155"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HIR5077586.1"
                     /translation="MAVTAQETLEYHRRNRPGKIEVVPTKPMLTQRDLSLAYTPGVAQ
                     VVLELERSPQAAYEYTAKANLVAVVSNGTAILGLGDRGALASKPVMEGKGVLFKRFAD
                     IDVFDIEVDTRDPHQLIHVVRAIAPTFGGINLEDVKSPECFFVEKTLQSLLDIPVFHD
                     DQHGTAIIASAGLLNALELTGRKIDQIKVVFSGAGAAAIATASMFVRLGVPREHIWMT
                     DVAGLVYRGRKKEMFPEKAAFAQGDRPATLADVLPDADVFVGLSVGGIVTKDMIRTMR
                     PAPIIFAMANPEPEIAYEDAKEARPDAIVATGRSDYPNQVNNVLGFPFIFRGALDVQA
                     RAIDEEMKIAAAQALAALAHEDVPDAVLNAYGLTSLKFDRDYIIPKPLDPRALLWVAP
                     AVAEAALRSGVARKPIDVDEYREQLAARMGQGRRLRRTMINQAKAAPKRIVFAEGEHT
                     TILRAAAAIVDEGIGHPILLGRPEVIHRQAQVLGLHFTPDAVDPNVDPRLEAYTESYY
                     KLRQRRGVTLGHARERIHEPNVFGLMMVRERDADAFISGLTYEYPEVIRPALQIFHTR
                     PGVGKAAGAYILLVRDQVYILSDATVNIEPGAEDLAEIAMLAADFAARLNLTPRVAML
                     SFSNFGSVPHPLAEKVRRATELVRARWPDLAIDGEMQADVAVMPELIESRFPFSRVKA
                     ANVLIFPDLEAANVTYKLLRRLGGAQIIGPILLGLGAPAHVLQPGDDVDEIVSIAAVA
                     VMGAQGKL"
     gene            complement(4713..5507)
                     /locus_tag="AC162G_17160"
     CDS             complement(4713..5507)
                     /locus_tag="AC162G_17160"
                     /inference="COORDINATES: protein motif:HMM:NF017946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077587.1"
                     /translation="MKRLRSELVFLIARWKANFASAMEYRAAFISQIVGMFVNDIVYV
                     AFWVVFFSQFQAVRGWGVSDIMLLMSIVGVGYGLAFTFFGNALQLSRIIAQGQLDYYL
                     ALPRSVLLNVLASRMSNSALGDISFGVVTYLLAGRFTPPEIGMWLVASLLAAVVFVMA
                     FTLFHSLTFWLGNASGLAEQAMNAMMTLAMYPSDIFQGAVRFMMLTVLPAAFVGVVPL
                     NVVRHLDLGLLALLAGAAIVITAVSSAVFYAGLRRYESGSALNVNV"
     gene            complement(5631..6431)
                     /locus_tag="AC162G_17165"
     CDS             complement(5631..6431)
                     /locus_tag="AC162G_17165"
                     /inference="COORDINATES: protein motif:HMM:NF017946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077588.1"
                     /translation="MSIARSIGKYTAITRINLQNNLAYAGELAYRSIFMVVILYVFVQ
                     LWTATYGAVGSDQIAGLRLSDTIWYLVTTETIMLSKIRFAGKVAEEVRDGSLAYTVGR
                     PYSYLLYHFFYGIGDTLLRLGINFAAGWILATLLVGPMPATNLPAVFVAVILAIVLDF
                     CINGMLGLLAFFTEDVDSFVIIYQKVLFILGGMLIPLDFFPAWLRDLSLALPFNTVIY
                     APARLFVQFDGSQWLRVVGMQLAWIALFALALGLMFRWGMRRVSINGG"
     gene            complement(6494..>6795)
                     /locus_tag="AC162G_17170"
     CDS             complement(6494..>6795)
                     /locus_tag="AC162G_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077589.1"
                     /translation="ILYDDRLSALKRRYLTRKEIDVRFAEALADGFAMNGVDVLKVGT
                     YGVKLQFDTHDMPVDAVIQRLMSERAVVDINVSDPPLEEIIARIYQEPGKGEIGD"
BASE COUNT         1249 a         2057 c         2211 g         1278 t
ORIGIN      
        1 gatgcgcgcg agggagtcgg cggcgagctg gccgacgtac acgctggggt cggcgagccg
       61 cccgatcaga gccgggatgg cgctggccgc ctctccgtcg agcgcgccga gcgcgtagat
      121 ggcacacgcg cgcacgtcgg gatcgacgtc gtggcagcgg tcgatgagca aagcgatcgc
      181 ggcagccgag tcgatctcac ccagcgcgcg agtgacccac cagcggcagt cgccatcggg
      241 atgagagagc agatcgcgca agacgggcaa ggcggcatcg cccagcctgc cgagcgccaa
      301 tgccgcctct tcggcgcggt cgtcatcgcc ggaaaacaac gcttcgatca gggtcttgtg
      361 atctgccgcg gtcataatcg gccctcgaaa gtgtgttgcg tgttccgcca ttcaaaacga
      421 aacacgcaac acgttaaggt tgtcccagtt gctcgacagg gacattacgc cacttgtcgc
      481 cctcttcgat cagcatgatg gggatgtcgt cttgaatcgg gtatttgcgg tcgcagccag
      541 gctcctggca cacgagccac acgcctttga cgagcaccag gcgacccgga tcaggtcctt
      601 cgcgtcgagt cggcccgctg acgcagtgcg ggcaacggag gatttcgagc aggtcggtgc
      661 tgaccataca tttctcctgt gagggaatcg cgcgttgatt ttatcacatg cgaaatcgat
      721 cggcaattgt ttgacagccc tatgcccgca tggtatactg tgtttgcgtt gagggagatg
      781 ccctcaccag gagggatggc cgagcggtct aaggcggtgg tcttgaaaac cactatgggg
      841 gcaacctcat cgggggttcg aatccctctc cctccgccat cggatcggat gtccggggag
      901 atgccggagt ggacgatcgg agccgcctgc taagcggttg tgcgggtaaa actgcaccca
      961 gggttcgaat ccctgtctcc ccgcctggtc ggagggtctc cggaccccga tacacgcccc
     1021 tgtagctcag cggatagagc gcagccctgc ggaggctgag gcccaggttc aattcctggc
     1081 aggggtacct ataccgatga ctacgctcca cgtcttgagt gccgtgggcg tttttgtttt
     1141 gtcggttctg gttgcctcct cgcggtcctg gatggggatg tgaggttggg ggttggagat
     1201 tagagattag agattagaga ttggagattg gagattggag attgaagcca tgcggaaacg
     1261 caggtggttg acgatagtcg ttgccggcat tttggctttc tgccttctgg ggtgggcttt
     1321 ggcgtattac ttcgtcggcg tcgatgtgag tttcattgca gggatggtgc gggcgcgcat
     1381 ggcggatggg agacaggcca tgcccgtcgc gccggcgggt gtcgaggtga cgacggttgc
     1441 cgatgagctt gacgcgccga cgtcgctggc ctggtcgccg gacggcgcga tctacgtcgc
     1501 ggacttcggg gcggggaagg tgtatcgttt cgccgtgccc tgagcgtgct cgtaagattc
     1561 tcgctgacac aactcgaaac gtgccttttt gtcatgccct ttgctataat atgcgcggat
     1621 aacccggcga aggcattgtt gcccagattc tcgcagcggc gatcgacgcc tagcgatgtg
     1681 ggtccttgag ccaggtgtga tatgatcgag ttatgaggag gacagtcgca tgaactggaa
     1741 ggaacgcatc gtcgttgatc cgagcatcct ggttgggaaa ccggtcgtta agggcacacg
     1801 cctcgcggtc gagtttatcg tcgatctctt ggcgcaaggc tggagcgagg cggacctgct
     1861 gcgcaactac cccggactga cgcatgaaga cattcaagcg tgcctgagtt acgccagcgc
     1921 cattctgaaa gccgaaaagg tctatccctt cgagatgtcg caggcgtagc ctatgcgcat
     1981 cctggcgaat gagaacttcc ctggcgacgc cgtcaatgcc ctgcgcaatc aagggcacga
     2041 cgtggcatgg gtgcgcaccg acgcgccggg caccagcgat gtcgatgtgc tggcgcacgc
     2101 gcaacgcgag aaccggatcg tcgtcacgtt cgataaagac tttggcgaat tggctttccg
     2161 agcgcggctg ccggcatcga gcggcatcat cctgttccga atctcggctc catcgccggc
     2221 acacgtggcg cgcgtcgccg ttgccgccct cgaaagccgg acggattggg ccggccactt
     2281 taccgtcgtc gaggataggc gtattcgcat gactcccttg ccttgactga tgcagtcaac
     2341 tgtggtggcc gctgcctgcc gcacgaaaag acacctagac tctcagcagt cttggtgtct
     2401 tcgtgtcttg gtggtattaa atagcttcgc gcccttcgcg tcttcgcggt taaagttttc
     2461 cttgcgcgcc catcaccgcg acggcggcga tcgagacgat ctcgtcgacg tcgtcgcccg
     2521 gctgcagcac gtgggcaggc gcgccgaggc caagcaggat cgggccgatg atctgagcgc
     2581 cgcccagccg ccgcagcagc ttgtaagtga cgttggccgc ttcgaggtcg gggaagatca
     2641 ggacgttggc cgctttcacg cggctgaagg ggaagcggct ttcgatcagc tcgggcatca
     2701 cggcgacgtc ggcctgcatc tcgccatcga tcgccaggtc gggccagcgc gcgcggacca
     2761 gctcagtcgc gcgccggact ttctccgcca gcgggtgcgg cacgctgccg aagttcgaga
     2821 acgacagcat cgccacacgc ggtgtgagat tcagccgcgc ggcgaaatcg gcggcaagca
     2881 tcgcgatctc ggccaggtcc tctgcgcccg gctcgatgtt cacggtcgca tcgctgagga
     2941 tgtacacctg atcgcgcacg agcaaaatgt acgcccccgc cgccttgccg acgccgggac
     3001 gggtgtggaa gatctgcagc gcgggccgga tgacctcggg atactcgtac gtcaggccgg
     3061 agatgaacgc gtccgcgtcg cgctcgcgca ccatcatcag cccgaacaca ttcggctcgt
     3121 gaatacgctc gcgcgcgtgc ccgagcgtga cgccgcgacg ctggcgcagc ttgtagtacg
     3181 actctgtgta tgcctccagg cgggggtcca cattggggtc gaccgcgtcc ggcgtgaagt
     3241 gaagacccaa cacttgcgcc tgtcgatgga tgacctcggg ccggccgagc aggatcggat
     3301 ggccgatgcc ttcgtcgacg atcgccgctg ccgcgcgcag gatcgtcgtg tgctcgccct
     3361 cggcgaagac gatgcgcttg ggtgcggctt tggcctggtt gatcatcgtg cgacgcaggc
     3421 ggcggccctg acccatgcgc gccgcgagct gctcgcggta ttcgtcgacg tcgatcggct
     3481 tgcgggcgac gccgctgcgc agggcggcct cggccaccgc cggcgcaacc cacagcagcg
     3541 cgcgcggatc gagcggcttc gggatgatgt aatctcgatc gaacttgagc gatgtcaggc
     3601 cataggcgtt gagcacggca tcgggcacgt cttcgtgcgc tagcgcagcg agcgcctgcg
     3661 cggcggcgat cttcatctcc tcgtcgatag cgcgcgcctg cacgtcgagc gcgccccgga
     3721 agatgaacgg gaatcccagc acgttgttga cctggttggg ataatcggac cgcccggttg
     3781 cgacgatagc gtcggggcga gcctctttgg cgtcttcgta cgcgatctcc ggctcgggat
     3841 tggccatggc gaagatgatc ggagcgggcc gcatcgtcct gatcatgtct ttggtcacga
     3901 tgccgccgac ggataagccg acgaacacgt cggcgtcggg cagcacgtcg gcgagcgtgg
     3961 ccggccggtc accctgcgcg aacgccgcct tctctgggaa catctccttc ttacggccgc
     4021 gatacaccag cccggcgacg tcggtcatcc aaatgtgctc gcgcggcacg cccaggcgga
     4081 cgaacatgct ggcggtggca atcgccgccg cgcccgcgcc ggagaagacg accttgatct
     4141 gatcgatctt gcgtcccgtc agctccaggg cgttcagcag gccggccgag gcgatgatcg
     4201 ccgtgccgtg ctggtcgtcg tggaacacgg ggatgtcgag caggctctgc agcgtcttct
     4261 cgacgaagaa acactcggga gacttgacgt cttcgaggtt gatgccgccg aaagtcggcg
     4321 cgatggcccg gacgacgtgg atgagctgat gcgggtcgcg tgtgtcgact tcgatgtcga
     4381 acacgtcgat gtcggcgaag cgcttgaaca gcacgccctt gccttccatc accggcttcg
     4441 acgccagcgc gccccggtcg cccaggccga ggatggccgt gccgttcgag acgacggcga
     4501 cgagattggc cttggccgtg tattcgtagg cggcctgggg agatcgttcc agctccagca
     4561 cgacctgcgc gacgccgggg gtgtaggcca gcgacagatc gcgctgcgtc agcatcggct
     4621 tggtcggcac gacttcgatc ttgccggggc gattgcggcg atggtattcg agcgtttcct
     4681 gcgcggtgac ggccatggat gtcctttccc cttcacacgt tgacgttgag cgcgctgccc
     4741 gattcgtagc gccgcaggcc ggcgtagaac accgcgctcg acacggcggt gatgacgatc
     4801 gcagcgccgg ccagcagggc aagcagcccc aggtcgagat ggcgcacgac gttgagtggc
     4861 accacgccga cgaacgccgc cggcagcacg gtgagcatca tgaagcggac cgcgccctgg
     4921 aagatgtccg acgggtacat ggccagcgtc atcatcgcgt tcatggcctg ctcggccagt
     4981 cccgacgcat tgcccagcca gaatgtcagc gaatggaaca gcgtgaacgc catgacgaac
     5041 acgacggcgg cgagcagcga cgcgaccagc cacatgccga tttcgggcgg cgtgaaccgg
     5101 ccggcgagca ggtatgtgac gacgccgaac gagatgtcgc ccagcgccga gttgctcatg
     5161 cgcgaggcca gcacattgag cagcacgctg cgcgggagcg ccaggtaata atcgagctgg
     5221 ccttgggcga tgatgcgcga gagctgcagc gcgttcccga agaacgtgaa ggccaggcca
     5281 tagcccacgc cgacgatgct catcagcagc atgatgtccg acacgcccca gcctcgaacc
     5341 gcctggaact ggctgaagaa cactacccag aacgcgacgt acacgatgtc gttcacgaac
     5401 atgccgacga tctggctgat gaaagccgcg cggtactcca tcgccgacgc aaagttggct
     5461 ttccaccggg cgatcaggaa gacgagttcg ctgcgcaggc gtttcatgag ttagagttcg
     5521 gagttagagt tagagtggtg ggcgtttttc tggattcttc attcgacatt ccgcactctt
     5581 cgttccacat tctacattct tcattccaca ttctgcattc accttcttcg tcagcctccg
     5641 ttgatggata ctcttctcat gccccagcgg aacatcaagc cgagcgcgag ggcgaagagg
     5701 gcgatccacg cgagctgcat cccgacgacg cgcagccatt ggctaccgtc gaactgcacg
     5761 aacagacgag ccggcgcgta gatgacggtg ttgaacggca gcgccagcga caggtcgcgc
     5821 agccaggccg ggaagaagtc gagcgggatc aacatgccgc ccaggatgaa caggaccttc
     5881 tggtagatga tgacgaaaga atccacgtct tcggtgaaga aggcgagcag cccgagcatg
     5941 ccgttgatgc aaaagtcgag gacgatggcc aggatcacgg cgacgaacac ggcgggcagg
     6001 ttcgtcgccg gcatggggcc gacgagcagc gtcgccagta tccacccggc agcgaagttg
     6061 atccccaggc gcagcagtgt gtcgccgatg ccgtagaaga agtgatacag caggtacgag
     6121 tagggccgcc cgacggtgta cgccagcgag ccgtcgcgca cctcctcggc cactttgccc
     6181 gcgaagcgaa tcttgctcaa catgatcgtc tcggtcgtca ccagatacca gatcgtgtcg
     6241 gacagcctca gcccggcgat ctggtccgat ccgaccgcgc cgtacgtcgc ggtccacaac
     6301 tggacgaaga cgtacaggat gacgaccatg aagatcgagc ggtacgccag ctcgccggcg
     6361 taggccaggt tgttctgcag gttgatgcgc gtaatcgcgg tatacttgcc gatcgagcga
     6421 gcgatactca ttgctccaac ctccaaacct ttggattttg aattttggag tttgaagtta
     6481 tctcctccaa tctctaatct ccaatctctc ccttccccgg ctcctgatag atgcgcgcga
     6541 tgatctcctc cagcggcgga tctgatacgt tgatgtccac tacggcgcgc tcggacatca
     6601 gccgctgaat gacggcgtcg accggcatgt cgtgcgtatc gaactgcagc ttcacgccgt
     6661 acgtgccgac cttgagcacg tcgacaccgt tcattgcgaa gccatctgcc agcgcctcgg
     6721 cgaagcgcac gtcgatctcc ttgcgtgtta ggtaacgccg cttgagcgcc gacaggcggt
     6781 cgtcgtacag gatcg
//
LOCUS       DBNHXB010000436         5684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4160455, whole genome shotgun sequence.
ACCESSION   DBNHXB010000436 DBNHXB010000000
VERSION     DBNHXB010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4160455"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="AC162G_17175"
     CDS             <1..233
                     /locus_tag="AC162G_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077590.1"
                     /translation="VVVRIIVIHTPPPPTPTPPPPPPTGGKVSLCHIPPGNPGARHTI
                     KVGPSAVSTHLGHGDTLGPCGEGDEDEHDEDD"
     gene            587..3025
                     /locus_tag="AC162G_17180"
     CDS             587..3025
                     /locus_tag="AC162G_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077591.1"
                     /translation="MDIPRLYTEASIKTADNLRTIARSATLRDLSRLSLPEIDAVVDL
                     VARVVPAGNVPGVILSGLARLAGRRPPLKTVQADINLLFKGVERVLDKAIYGAFFAGP
                     AAVIWGYQNLLKLAGKDPEDSFPEGTWQFYADYALRDDTARHANETHGFDTALNQHHI
                     RLSAADRITAWLMAAIHCLHQYDALLENEWRERAYTYLLREPAGRGPGAARYAGLYRD
                     WERQRPYARGSDAAARHDYPTYRRLKFDRFLEDTMRDCRADLRREWNERVRAAEDHDL
                     PAYRRQMSILAYLDPGAYGETRTPISLQAAHVGVIYRGRYTLIPACSPGTDRPADVTS
                     VRAQVAALVADPSDAPRAQLTSLARVKRGAWPGLRSKMSQTLIADLDALRSAPILLNC
                     DPRPRHLPLSDLRQTERGVGDHALTIFDTGETFVFDQSHIFFDGAWGAALAEIMTNEA
                     LSWAVYLNALPPARPGQTRPTMPAFKLNASEQKIIQQAPHVTAEVSAETEAVDLKAIL
                     ELRKFFKQRSDLLQLTVNDLLVFYRAIHAVTYRPDPDLVAELNRLAHDSAAQRAAQAG
                     LDAIDPSQQVNPAILIPIDASQRSPRDRVYPMSFEAPLDDLDLLNLHQRVMAALDAYK
                     SATHRADRAALYAEFDRWQRTYLATLAGFGAVLSKAKEIALTGESATVGTIKLLAHLP
                     ASLQRLLDRIPGRFDVLNDIIKGREVFSNVGAVAPTSTLIRFISAKDDNEKKTLAWGV
                     ITSAGGVMRISLRDFRPHVGLLEAVGHRDLAVRLCQDYLEAYARGLNATVADLRRMTL
                     SSRETLLAAQPERK"
     gene            3022..4749
                     /locus_tag="AC162G_17185"
     CDS             3022..4749
                     /locus_tag="AC162G_17185"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5077592.1"
                     /translation="MIAGDPLSRRQLANYRIERVIGRGGMAQVYYGWDVVLHRPVALK
                     IIDARYQGSPAYAKRFVQEAQTVASWRHENIVSIYYAGQEGELYYFAMEYIDGVDLGV
                     RMSQYTTAGQLMPHTEAVRIGRAIASALDYAHRKGVIHRDVKPSNVILASDGRVVLTD
                     FGLALHVQQGSLGEVFGTARYTAPEQARRSADAVPQSDLYSLGVILYEMLTGSLPFDD
                     PSPTSVALQHMTLSPPPPRAINPDLNDAAEAVLLKALSKSPGDRYPTGGELMDALESA
                     LLTRRPAGSQLPAARPVPQRSVAERVASYLQADTLSPDVVHPTQSMTGSDSGRRRDPG
                     AFLATKGRLLILAAIAVVAGTMIVINQQSNASQPPTLAPARATTGTSPTTTAIPTATP
                     TARGQAIAPAETALPGTAGTDASTPTLAPTPGFIPPTATEPLPATPLPTVLYPDGRRF
                     LIYYDDSGLYLHNASGSAGAVSPLAFERLDASGAPLNRFDGWRWSRYYPRIEVASCVR
                     VEIRRSSPYLRPSPCNNRYNSTVTASRNDDFVFWTTQTGASQFRVLWNAEEVARCGIA
                     TGVCEVFLP"
     gene            4746..>5684
                     /locus_tag="AC162G_17190"
     CDS             4746..>5684
                     /locus_tag="AC162G_17190"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5077593.1"
                     /translation="MTTDSLLGKQLDEYRLEVLLGQGGMARVYRGLDVRLNRYVAVKV
                     IDAPFRADPDYVVRFEREAQAIAQLDHPHIVRLYRYGEAEGLLYIAMQYIRGASLSYV
                     LDSYRADKEFIAPNDAQRIMREVCLALDYAHSHGVIHRDIKPANIILDQQGRAYLTDF
                     GLALLTEVGTRGEILGSPHYIAPEQAISSAAAVPQSDLYSVGVILYEMFTGKLPFDAE
                     EPLEIARLHMTEPPRPPRDLRPELSRGVEAVLLRALAKEPDERYPTGAALADALDEAL
                     RAAPVPLPSPPTLARPSIPERVKAERARRPLPPLPAA"
BASE COUNT         1073 a         1883 c         1704 g         1024 t
ORIGIN      
        1 tcgtcgtcgt gcgaatcatc gtcattcaca cgccgcctcc gccgacgccg acaccaccgc
       61 cgcctccgcc cactggtggg aaggtttccc tctgccatat cccgccgggc aatccgggcg
      121 caaggcacac tatcaaggtc ggcccatccg ctgtgtcgac ccacctcggc cacggcgaca
      181 cgctcggccc gtgtggtgag ggtgacgagg atgagcatga cgaggatgac tgagccaatg
      241 aaccggggca accggcaagt acacccatga cgtctcggac aaaggcataa cacgctggag
      301 cacaagagga aaagctcacg atccggcagg ccgggtttct acacccggcc tgccgctctg
      361 tcatctacgc tcaggcccat gcgccacgtg aaaaaggaga atatggcaac gatggcatct
      421 tgatgtatac tagagtggtt tctgattccg tttacgctcc actggagtcg agcctttagg
      481 cagtttccgg ctaaagcctc gactccggcg taaaccaaac aggaattcgc tctaaacgca
      541 tacaggcaga tcgcctccta tttccggccg cggtaggagc cgcactgtgg acatccctcg
      601 gctgtatacc gaagcgtcga ttaagacggc ggacaatctc agaaccatcg cgcgctcggc
      661 cacgctgcgt gatctgtccc ggctgtcatt gccagagatc gacgcggtgg ttgatctcgt
      721 ggcgcgggtg gtcccggcag gcaatgtgcc gggcgtgatc ctgagcggat tggcgcgcct
      781 ggcgggacgc aggccgccgc tcaaaacggt ccaggcagac atcaacctgc tgttcaaggg
      841 tgttgagcgg gttctcgaca aagcgatcta tggcgcgttc ttcgccgggc cggctgccgt
      901 catctggggc taccagaacc ttctcaagct agccggcaaa gatccggagg actccttccc
      961 ggaaggcacc tggcagttct acgcagacta tgccttgcgc gacgacaccg ctcgccacgc
     1021 caatgaaacg cacggcttcg acaccgccct caaccaacac cacattcgct tgagcgccgc
     1081 cgaccgcatc acggcgtggt tgatggcggc catccactgc ctgcatcaat acgacgcgct
     1141 gctggaaaac gagtggcgcg agcgtgcgta tacgtacctg ctgcgcgagc cggccggccg
     1201 cgggccaggc gctgcacgct acgccgggct ttatcgcgac tgggaaaggc agcgccctta
     1261 tgcacgtggt tcagatgccg ccgcccgtca tgactatccc acctatcgcc gtctcaagtt
     1321 cgaccgtttc ctggaagata cgatgcgcga ttgtcgcgcc gatctgcgcc gcgagtggaa
     1381 tgaacgggtg cgcgctgccg aagaccatga tctccccgct taccggcggc aaatgtccat
     1441 cctggcttac ctcgatcccg gcgcatatgg tgagacacgc acgcctatct cgctgcaagc
     1501 cgctcacgtc ggcgtcatct accgggggcg gtatactctg attccggctt gctcgccggg
     1561 caccgatcgg ccggctgacg tcacgagcgt gcgggcgcaa gttgcggccc tggtcgccga
     1621 tccctccgac gcgcctcgcg cgcaactgac ctcgctggct cgggtgaaaa ggggcgcgtg
     1681 gcccggcttg cgcagcaaga tgagccaaac gttgatcgcg gacctggacg cactccgatc
     1741 ggcgccgatt ctgttgaatt gcgatccccg gccgcgccac ctccccctgt ctgatctgcg
     1801 ccaaaccgaa cgcggcgtgg gcgaccacgc cctgaccatc ttcgataccg gcgagacttt
     1861 cgtctttgat cagtcccata tctttttcga cggggcatgg ggagccgctc tcgccgaaat
     1921 catgacaaac gaagccctgt cctgggcggt ttatctgaat gcactgcccc cggctcggcc
     1981 cggccaaacg cgccccacca tgcctgcttt caagctcaac gcatctgagc agaagatcat
     2041 ccagcaggct ccgcatgtga ccgccgaagt cagcgcagaa accgaagccg tagacctcaa
     2101 ggcgattctg gagctgcgca aattcttcaa gcagcgcagc gatttgctgc agttgacggt
     2161 caacgatctc ctggtgttct atcgcgccat ccacgccgtg acttatcgac ccgatccgga
     2221 cctggtggcg gagctgaatc gcctggccca cgacagcgcc gctcagcggg cggcccaggc
     2281 cggcctggac gcaatcgatc catcccaaca ggtgaatccg gccatcctca tccccatcga
     2341 cgccagccag cgttccccac gcgaccgcgt ttatccgatg agctttgaag cgccgctgga
     2401 cgacctggac ttgctgaacc tgcaccagcg ggtcatggcc gcgctggatg cctacaaaag
     2461 tgcgacacac cgggcggacc gcgccgcgct gtatgccgag tttgaccgtt ggcagcgcac
     2521 ctatctggca acattggccg gcttcggcgc ggtattgagc aaagccaagg aaatcgctct
     2581 cacgggcgaa agcgccactg tcggcaccat caagctgctg gcgcacttgc ccgcttcgct
     2641 ccagcgcctg ctggacagga ttccggggcg cttcgatgtg ctcaacgata tcatcaaagg
     2701 gcgcgaggtg ttctcgaatg tgggagcagt cgcgccgacc agcaccctca tccgctttat
     2761 cagcgccaaa gacgacaacg agaagaaaac gctggcgtgg ggcgtgatca cgagcgccgg
     2821 cggtgtgatg cgcatcagcc tgcgcgattt ccgaccacac gtcggcttat tagaggcagt
     2881 cggacataga gacctcgccg tccgcctgtg ccaggattat ctggaagcct acgcgcgcgg
     2941 attgaacgcc actgttgccg acctgcggcg catgaccctt tccagccgcg agacccttct
     3001 cgctgcgcaa ccggagcgaa aatgatcgcg ggcgatcctc tgagcagacg gcagctcgcc
     3061 aattaccgta tcgagcgcgt gatcgggcgc ggcggcatgg ctcaggtcta ttacggttgg
     3121 gacgtcgtcc tgcaccgtcc ggtcgccctc aaaatcatcg atgcgcgcta ccagggcagc
     3181 ccggcctacg ccaagcggtt cgttcaggaa gcgcagacgg tggcgtcgtg gcgacacgag
     3241 aacatcgtca gcatttatta cgcgggccaa gaaggtgaac tctattactt cgcaatggaa
     3301 tatatagacg gggtagacct gggcgtgcgg atgtcgcagt acacgacggc aggccagctc
     3361 atgcctcaca ctgaggccgt gcgcatcgga cgagcgattg ccagtgcctt agactatgcc
     3421 catcgaaaag gcgttattca ccgcgatgtg aaaccgtcga acgtgatact ggcgagcgat
     3481 ggccgggtgg tcttgaccga ttttggtctg gcgctgcacg tgcagcaggg ttcgctgggt
     3541 gaagtgttcg gcacggcgcg ctacacggcc ccggaacaag cccgccgctc tgccgacgcc
     3601 gtgccgcaat ccgatctgta ctcgctcggc gtgatcctgt acgagatgct gacaggcagc
     3661 ctgccgtttg acgatccttc cccgacgagc gtggcgctgc agcacatgac gctctccccg
     3721 ccgcccccgc gcgcgatcaa tcccgacctg aatgatgcag cggaagccgt tctgttgaag
     3781 gcgttgagca agtctcccgg cgatcgctac ccgaccggcg gtgagctgat ggatgcgctg
     3841 gagagcgctc tgctgacccg ccggccggcc ggttctcaac tcccggcggc acgcccagtg
     3901 ccgcaaaggt cggtggcgga acgagtcgcc tcttacctgc aggctgacac cctatctccc
     3961 gacgtggttc accccacaca atccatgaca gggagcgatt cgggccgccg acgagaccca
     4021 ggagcatttt tggcgacgaa aggtcgcctc ttgatcctgg ctgcgatcgc cgtcgtcgcg
     4081 gggacgatga tcgtgatcaa ccaacaaagc aatgcttcgc agccgcccac actggcgcct
     4141 gctcgcgcga ccacagggac ttcgccgacg acgaccgcca taccgacggc gacccccact
     4201 gcgcgagggc aggcgatagc gcctgccgaa accgctctac ccggcaccgc tggcacagac
     4261 gcgtctacac ccacgcttgc gccgacgccg ggcttcatcc cgccgacggc cactgagcct
     4321 ctacccgcga cgcctctgcc gaccgtgctc tatccggacg ggcggcgctt cctgatctac
     4381 tacgatgaca gcggcctata tctgcacaat gcatcgggca gcgccggcgc ggtctcgccg
     4441 ctggcctttg agcgcctgga cgcttccggc gcgccactga atcgcttcga cggctggcgc
     4501 tggtcgcgat attacccgag aattgaggtc gcgtcgtgtg tgcgcgtaga gattcggcgc
     4561 agctcgccgt acttgcgccc gtcaccatgc aacaatcgct acaacagcac ggtcacggct
     4621 tcacgtaatg atgactttgt cttctggacg actcagacag gcgccagcca gttccgtgtc
     4681 ttatggaatg cagaggaagt ggcacgctgc gggatcgcca caggggtctg cgaggtcttc
     4741 ttaccgtgac gaccgatagc ctgctcggaa agcaactgga cgaataccgc ctcgaggtct
     4801 tgttggggca gggcggcatg gcgcgagtct atcgcggact cgacgtgcgc ctgaaccgtt
     4861 acgtggccgt caaggtcatc gacgcgccgt ttcgcgccga cccggactac gtggtgcgct
     4921 tcgagcgcga agcccaggca attgcccagc tcgatcatcc ccacatcgtc cggctctacc
     4981 gctacggcga agccgagggc ctgctttaca tagcgatgca gtacattcgg ggggccagcc
     5041 tgagctatgt gctcgacagt tatcgagcgg acaaggagtt catcgcgccg aacgatgccc
     5101 aacgcatcat gcgagaggtc tgtctggcgc tcgactacgc tcacagccac ggcgtcattc
     5161 atcgagacat caaaccggcc aacatcatcc tggatcagca gggccgcgcc tacctgaccg
     5221 acttcggctt ggcgctgctc accgaggtcg ggacacgagg cgagatttta ggctctcctc
     5281 attacattgc cccggagcag gccatttctt ctgccgccgc cgtgccgcag agcgatctgt
     5341 actccgtcgg cgtcatccta tacgagatgt tcacgggaaa gcttcccttc gacgccgagg
     5401 agccgttgga gattgcgcgg ctgcacatga cggagcctcc tcgcccgcca cgagacctgc
     5461 gaccggagct gagccgggga gtggaagccg ttctcctcag ggcgctggct aaagagccgg
     5521 atgagcgcta cccgaccggg gccgccctgg ccgatgcgct ggacgaagcg ctgcgggcag
     5581 cgccggttcc attgccatcg cctcccacgc ttgcacgccc ttcgatccca gagcgcgtca
     5641 aggcggaacg agcgcgtcgg ccactgccgc cgcttccggc tgcg
//
LOCUS       DBNHXB010000437         9777 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1140675, whole genome shotgun sequence.
ACCESSION   DBNHXB010000437 DBNHXB010000000
VERSION     DBNHXB010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9777
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1140675"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="AC162G_17195"
     CDS             complement(<1..1075)
                     /locus_tag="AC162G_17195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR5077594.1"
                     /translation="MKLPILLFCVALGVRLAALVVWPFDGLYGQDAYDYYHYAVSLRE
                     SLAQGGGVPPFFWPIGYPLHIVAGQLIIGMQPSAGQAVSVIAGALVAPLTYALAHEAL
                     KLTDPRRARRAGLVAGLVVAVAGQMLISSISIMSDATGLAWATLSAWLVVRYARTLRP
                     ATLALVSLTLSLAVITRWVFGLLVLPWSLAVGLAWRRGWRLIGWQRGIALGLMACTIG
                     GSIVGAQLLGGDSHTGDLQVVGWNPANAFRSEVINTDGAWRYALPIGLFYLQPLVVPS
                     YVFPLFIPLWLAGLAALGRLDAPVRALLVGWPLVVYVFLAGIAWQNPRFALAFFPPLA
                     VWVGVGFDRIWERRPAWRKGLVGL"
     gene            complement(1072..1947)
                     /locus_tag="AC162G_17200"
     CDS             complement(1072..1947)
                     /locus_tag="AC162G_17200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5077595.1"
                     /translation="MGLRCPTCREPIDSRGQACPNGHSFEYRDGVLRLLDAAFADRLE
                     SFTARLSAIREAEGKRLTDVSAYEGLPYSQLESGDPAWRTEWRLRCHDLDILFRLVAG
                     RSRRRVLDVGAWNGWLSHRLAAQGHDVTAVDYFDDACDGLAARKHYRAHWRAIQMDLE
                     DLAVLDEPFDVIVLNRCLAFFPDPVAGVISARRLLAEDGTLVITGLQFFRDAAPRARF
                     IASQREAYRARYDFELFLKPTKGYFDWEDRRRLGEAGVVLKSYPQLWLANLRALARRA
                     LPWHMYGLAFAEPSA"
     gene            complement(1919..3163)
                     /locus_tag="AC162G_17205"
     CDS             complement(1919..3163)
                     /locus_tag="AC162G_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR5077596.1"
                     /translation="MLDETARSRLRRRASPVILNAASSLLIPLLSPIVSLAVVRLAGA
                     SLWGDFVGVLIVVQLVAHVVAWGNKEYLLRRFSFSPAHAAHAWQSSLATRLLLVVAPL
                     GVTVVLALSGFALNRLAPAILWTLGLVVYQSFDVIVLYKRSFVFSGAVELAGLGLMLA
                     AILRREASIDLDSLIVLFGVVTWLKAIAMAIRFRRPFLAGAFRRPLLSGRFDRRYFAQ
                     AFPFFALGLTGLLQSRIDLYMANYFLPRSELAQYQVFMTFVLFLQSVSNFVLAPFVKN
                     IYRAQVRSILGLSPRLLALGVLVVLPGLLAVWAILKMHYGFDLPSGTFILGGIAALPI
                     FFYLPMIYALYKVGAQSEVVKLNLIGVGVSFALSVALLPRVGLAGAVGAAAAAQCCMA
                     AAYSLRSRSLRAGYGIAMPDLS"
     gene            complement(3184..4428)
                     /locus_tag="AC162G_17210"
     CDS             complement(3184..4428)
                     /locus_tag="AC162G_17210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HIR5077597.1"
                     /translation="MIGRIGHVNARANVLLRPALIIIVLLAGLFFFVDTLLPARRAVT
                     HGFPGYYTASRLLIEGRWGPAAYDDNWFGDRVAELTGNRVREIITPNTPLVALVLVPL
                     ARLDMSVAREIWTWLNLLLFAGSLWLIGNALPAPASLTWRAGFVALALLYAPAREDFR
                     LGQMYVLLLFLLSLAFWCLQRRSTAIAGLPLGIATATKLSVVPLWLILAGRGDRRTLV
                     IGALTMLGACALGIALTGWASWPRFIEAVIERVADYPAQAATAFQSTPGFFQHLFVRE
                     ATWNPAPVWHQPWLAHLLTPFASVTTIGATLWRARRVDIDLVLASGLALSVVLLPLAE
                     EHHYTLLLIPIGVVAARITRTPLLRRDVLWLSAIILLLAVAWPYESPSLSTGWAALLA
                     YPRLYGGWLLWAWLYVRMKTHG"
     gene            complement(4429..5904)
                     /locus_tag="AC162G_17215"
     CDS             complement(4429..5904)
                     /locus_tag="AC162G_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR5077598.1"
                     /translation="MIILYNPPSSAQRKPVLPMSLLALGALLEGEHEYRIVDGNLVGD
                     TVAEVDRVVRETGADILAVTVMPGPQLGDAVPLCRVVKARHPHLTIVWGGYFPTQHFD
                     VCLRSGYVDYVVCGHGEAVFKSLVDVLRRGDDPTGLPGLAFRRPGSGQIISNPTPPIP
                     HPNRLPDWPYHRVDAARYLRRTFMGERTLPHHSSYGCPFFCNFCAVVNMVNGRWLAQS
                     AERTAATVQRLVHEFGVDAVEFYDNNFFTAEARTAEFAERVRRLGIGWWGEARIDTLM
                     HYSHRTWDLMRGAGLRMVFLGAESGSDDTLKRMNKGGQASTEKTLELAARMKQYGIVP
                     EMSFVLGNPPNPDEDVRQTIEFIRKVKRVNPEVEIILYMYTPVPLAGELYEEAKAEGF
                     QFPETLEQWISPEWQEFSQRRSTNVPWLNDPVRRKIRNFQWVINAYYPTITDLRHSKL
                     RRQMLRALSAWRYHLRLYGYPLELRAFNRLLAYQRPETSGF"
     gene            complement(5901..6575)
                     /locus_tag="AC162G_17220"
     CDS             complement(5901..6575)
                     /locus_tag="AC162G_17220"
                     /inference="COORDINATES: protein motif:HMM:NF017026.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIR5077599.1"
                     /translation="MPRLFVRFLLWLRFWLFQRLRHNRLVLEYVNGRPFLVLPGVFNP
                     TLFRSSELFAKSLSSEWIRSGSTVLDMGTGSGIGAVYAAQWAAHVVAVDVNPEAVCCA
                     RINVLLNHVDDRVEVREGDLFEPVCDMRFDAILFNPPFFRGRPRDVSDFAWRSEDVAE
                     RFAAGLRGRLAPGGCAWVILSSLGDDEAFLRAFRANGFQADVMLRRDLLSEVMTIYRI
                     GQGPCV"
     gene            complement(6575..7537)
                     /locus_tag="AC162G_17225"
     CDS             complement(6575..7537)
                     /locus_tag="AC162G_17225"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR5077600.1"
                     /translation="MRDAHVGQIDRDRLTFACPVCRARLVRESPDALRCPNDAEAYFR
                     VDGIWRLLPPQRASALAQFAREYETVRRDEGWGSPDAGYYRALPFNDVSGRFADLWRI
                     RAKSFQALVERVVKPLEWTWRRPLTALDLGAGNGWLSHRLARRGHGVAAIDLLINSTD
                     GLGAHVHYDAAFVPVQAEFDRLPFDDDQADLVVFNGSLHYSLDYETTLCEALRVLRRD
                     GCLAIVDSPVYREAASGERMVQEREARWMRLYGFRSHALPHENYLTYARLDDLAARLG
                     LCWQVAHPSFGWRWAVRRGWARLRVRREPARFPLIVGRHAEARA"
     gene            complement(7530..8360)
                     /locus_tag="AC162G_17230"
     CDS             complement(7530..8360)
                     /locus_tag="AC162G_17230"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR5077601.1"
                     /translation="MRDAIRDTPSAIGHQPSAFDQVAFDYDRQFTDTQLARVLRSRVW
                     ERLVVHIRSGMHVLEIGCGTGEDAVWLAERGARVVATDISPAMLEVARRKAERAGVAD
                     RIDTRILDAASPAAVFSEGEFGMAVSNFGVLNCVPDLHPVANALAAWLRPGGWLVVVF
                     INRWCGWEIAWHVTHGQPRVAFRRLRRSVEARVGEATVPVWYPSVASIRRVLAPRFML
                     RQLTGLGVFLPPSYLEPVVARRPRLLDLLARLEWATATVFPFNRLADHVILEFQRTDA
                     "
     gene            complement(8416..>9777)
                     /locus_tag="AC162G_17235"
     CDS             complement(8416..>9777)
                     /locus_tag="AC162G_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR5077602.1"
                     /translation="EDPHELKVMKPYPPLGILYISAYLKRVGYHVGVLDTTFGSMADF
                     EATIERERPPVVGLYTNLMTKFNVLKLIAVCKRLGATVILGGPEPACYVEEYLDRGAD
                     VIVAGEGEAALEELLPRLAASRDGPHRLDGIPGIAYRRDDGVIVRALPRTYIADLDSL
                     PDPDREAIDIERYVETWRAHHGMGSVSLICARGCPYHCEWCSHAVYGHTHRRRSPGRV
                     ADEVEDLLSRYKPDQLWYADDVFTIKHGWFFEYAAELKRRGIRTPFECISRADRLNEQ
                     VIDTLAEMGCYRLWIGSESGSQKVLDEMRREVKVEQVQAMIHALKRRGIQTGMFIMLG
                     YEGEEIEDLEVTVDHLKKSTPDVFLTTVAYPIKGTAYYEKVEDRVLARADWDRRTDRQ
                     LTVKGRHSMRFYSFATRWMVNSVALHRERKNGASMTRLAKAAANVALGRAGMMLTAGE
                     RET"
BASE COUNT         2095 a         3235 c         2956 g         1491 t
ORIGIN      
        1 ccaggccgac cagccccttc cgccatgccg ggcggcgctc ccagatccga tcgaacccga
       61 cgccgaccca gaccgccagc ggcggaaaga acgcgagcgc aaaacgagga ttctgccagg
      121 caatcccggc cagaaagacg tacacgacca gcggccagcc caccagcaga gcgcggacgg
      181 gagcgtctaa tcggccgagc gcagccagac cggccagcca caatggtatg aacagcggaa
      241 atacgtagct cggaacgacc agcggttgta ggtagaacag gccgatcggc agtgcatagc
      301 gccacgcgcc atcggtgttg atcacttcgc tgcgaaaagc attggccgga ttccagccca
      361 cgacctgcag atcgccggtg tgcgaatcgc cgccgagtaa ctgtgcaccg acgatcgatc
      421 cgcctatcgt gcaggccatc agcccaagcg caatcccgcg ctgccagccg atcaagcgcc
      481 agccacgccg ccaagccaga ccgaccgcaa gtgaccaagg cagcaccaac aggccaaaca
      541 cccagcgcgt gatgacggca agcgacaagg tcagcgacac aagggccagc gtcgccggtc
      601 gaagcgtgcg cgcgtatctc acgaccagcc aggccgacaa ggtcgcccaa gccaatcctg
      661 tagcgtcgga catgatcgag atacttgaga tgagcatctg tccggccacg gcgacgacga
      721 gcccagcgac cagcccggca cgccgtgcac ggcgcggatc ggtcaacttc agggcctcgt
      781 gcgccagcgc gtacgtcaaa ggcgcgacga gcgcgccggc gatgacgctg acagcctgcc
      841 ccgcggaagg ctgcataccg atgatcagct ggccggcgac gatgtgcaat ggataaccga
      901 tcggccaaaa gaagggcggc acaccgccgc cctgtgccaa gctttcgcgc agactcacgg
      961 cgtagtgata gtaatcgtac gcatcctgac cgtacagccc gtcgaacggc cagacgacaa
     1021 gcgcggcgag ccgcacgccc agcgctacgc agaacaacag tatcgggagc ttcatgcact
     1081 cggctccgcg aaggcaagac cgtacatatg ccagggcaaa gcacgcctgg ccagggccct
     1141 gagattcgcc agccagagtt gaggatatga cttcagcacg acgccggctt cccccaaccg
     1201 ccgtctgtcc tcccaatcga aatagccctt cgtgggcttc aggaacaact cgaagtcata
     1261 acgcgcacga tacgcctctc gctgtgacgc gatgaagcgc gctctaggcg cggcatcgcg
     1321 aaagaactgc aatcccgtga tcacgagcgt gccgtcctcc gccagcaacc ttcgcgccga
     1381 gatcacaccc gcgaccggat cagggaagaa cgccaggcag cggttaagca cgatgacgtc
     1441 gaagggctcg tccaagaccg ccaagtcttc gagatccatc tgaatagccc gccagtgcgc
     1501 acgataatgt tttcgcgccg cgaggccgtc gcaagcgtcg tcgaagtagt cgaccgccgt
     1561 cacgtcgtgt ccttgcgccg ccaaccggtg gctcagccag ccgttccacg cgccgacgtc
     1621 gagcacgcgg cggcgcgagc gaccggcgac caggcggaac agaatgtcga gatcgtgaca
     1681 tcgcagtcgc cattcggtgc gccacgccgg gtcgccgctc tcgagctgcg agtacggaag
     1741 tccctcatat gcagagacgt ccgtcaaccg cttgccctcc gcctctcgga tcgcgctcag
     1801 tctggccgtg aacgactcca gccgatcggc aaacgcggcg tcgagcaggc gcagaacgcc
     1861 gtcccgatac tcgaatgaat gcccgttcgg gcacgcctgc ccgcgcgaat cgatcggctc
     1921 acgacaggtc gggcatcgca atcccatagc cagctcgcaa cgaacgactt cgcagcgaat
     1981 acgcggcggc catacagcac tgcgccgccg cggcagcgcc cacagcgcct gccaggccaa
     2041 cccgaggaag caaggccacg ctcagcgcga agctgacgcc tacgccaatc aggttaagct
     2101 ttaccacttc tgattgcgcg ccgaccttgt acagggcgta aatcatcggc agatagaaga
     2161 aaatcggcag cgccgcgatc ccgcccagga tgaacgtgcc ggatggcagg tcgaatccat
     2221 aatgcatctt caagatagcc catacggcga gcaggcccgg cagcacgacg aggacgccga
     2281 gcgcgagcag ccggggcgac aggcccagaa tcgagcgcac ttgggcgcga tagatgttct
     2341 tcacgaacgg ggccagcaca aagttggata cggattgcag gaagagcaca aacgtcatga
     2401 acacctgata ttgagccagt tcactgcgcg gcaggaagta gttggccatg tacagatcga
     2461 tgcgcgattg cagcaggccg gtcaggccga gcgcaaaaaa gggaaacgct tgcgcgaagt
     2521 agcgtcgatc gaagcgcccg ctcaacaacg gccgtcgaaa cgcgccagcg aggaaaggtc
     2581 gccggaaccg gatcgccatc gctatcgcct tgagccacgt cacgacgcca aacagaacga
     2641 tcaggctgtc cagatcgatg ctcgcctctc gccgaagaat cgcagccagc atcaagccca
     2701 aacctgccag ctcgaccgcc cccgaaaaca caaagctcct cttgtacagc acgatcacgt
     2761 cgaaagattg atataccacc agcccaagcg tccacaggat agccggcgcg agccgattca
     2821 aagcgaatcc gctcagggca agcacgacgg tcacgcccaa cggggcgacg actagcagca
     2881 atcgcgtcgc caggctcgat tgccaggcat gcgcggcatg ggctggactg aagctgaacc
     2941 ggcgcagcag gtactccttg ttgccccagg cgacgacatg ggcgaccaac tggacgacga
     3001 tcaagacgcc gacgaagtcg ccccacaggc tcgcgccggc aagccgaacc acggcgagcg
     3061 acacgatcgg gctgagcagt gggatcagca ggctgctggc cgcattcaaa atcacaggag
     3121 acgcccgccg ccgtaacctg gagcgggcgg tctcgtcgag catgttttgc atcctcatcg
     3181 cgatcatccg tgcgtcttca tacggacgta cagccacgcc cacagcagcc atccgccata
     3241 aaggcgtgga taggccagca gcgccgccca acccgtgctg agactcggtg attcgtaagg
     3301 ccacgccaca gccaggagca ggatgatcgc cgagagccag agcacgtcac gtctaagaag
     3361 cggcgtgcgg gtgatgcgtg ctgcgaccac gccgatggga atcaaaagca gggtgtagtg
     3421 atgctcctcg gccaggggca gcaaaacgac gctcaatgcc aggccggatg ccaacacgag
     3481 gtcgatatcg acgcggcgag cgcgccacag cgtagcgccg atagtcgtga cagaggcgaa
     3541 aggagtcagc aggtgcgcca gccacggttg atgccacacc ggggccgggt tccacgtggc
     3601 ctcacgcacg aacaggtgct gaaagaatcc tggcgtggac tgaaatgccg tggctgcttg
     3661 ggcaggataa tctgcaacac gctcaatcac cgcctcgatg aagcgcggcc agctcgccca
     3721 gcctgtcaag gcgatgccga gcgcacacgc gccgagcatt gtcagcgcgc ctatgacgag
     3781 cgttcggcga tcccctcgac cggccagaat cagccacagc ggcacgacgc tgagtttcgt
     3841 cgccgttgca atgcccaagg gcaggccagc gatcgccgtc gacctgcgct gcagacacca
     3901 gaacgcaagc gacagcagga acaagagcag cacatacatc tgccccaggc gaaaatcctc
     3961 tcgggcaggc gcgtagagca acgccaaggc tacaaaaccg gcgcgccagg tcaggctcgc
     4021 aggcgctggc agcgcgttcc cgatcagcca caggctgccg gcaaacagta acaggttgag
     4081 ccacgtccat atctcacgcg cgacggacat gtcgagccgc gccaggggaa cgagcacgag
     4141 cgcgacaagc ggcgtgttgg gggtgatgat ctcacgcacg cgattgccgg tgagctcggc
     4201 tacccggtcg ccgaaccagt tatcatcgta tgcggctggc ccccagcggc cctcgatgag
     4261 taaccgcgag gcggtgtagt agccgggaaa gccgtgcgtc acagcgcgcc gggccggcag
     4321 cagcgtgtcg acgaagaaga aaagcccggc gagcagtaca atgatgatca gcgccggcct
     4381 taggagcaca ttcgcacggg cattgacgtg tccgatgcgc ccgatcattc agaacccgct
     4441 cgtctctggg cgctgataag ccaggagtcg gttgaaggcg cgcagctcga gcgggtagcc
     4501 gtacaggcgc aagtgatagc gccacgcgct cagcgcgcgc agcatctgcc gccggagctt
     4561 cgaatgacgg aggtcagtga ttgtcggata gtaggcgttg atcacccact ggaagtttcg
     4621 gatcttccgg cgcacaggat cgttcagcca tggaacattc gtgctgcgtc gctgcgaaaa
     4681 ttcttgccat tccgggctga tccactgctc cagcgtctca gggaactgga atccttccgc
     4741 cttcgcttct tcgtatagct cgcccgccag aggaacaggc gtgtacatgt acaaaatgat
     4801 ctcgacctcg ggattaacgc gcttcacctt gcggatgaac tcgatcgtct gccgaacgtc
     4861 ctcgtccgga ttgggcgggt tgccgagcac gaaagacatc tcaggcacga tgccatattg
     4921 cttcattcgc gcggccagtt ccagtgtctt ctcggtcgac gcttggccgc ccttgttcat
     4981 gcgcttgagc gtgtcgtccg atcccgattc agcaccgagg aaaaccatac gcagcccggc
     5041 gccgcgcatg agatcccagg tgcgatggga gtagtgcatc agcgtgtcga tgcgcgcctc
     5101 gccccaccag ccaatgccga gacgccgcac gcgctcggcg aactccgcag tgcgggcctc
     5161 ggccgtgaag aagttgttgt cgtagaactc caccgcgtcg acgccgaact cgtggaccag
     5221 gcgctgcacg gtcgccgcgg tgcgctctgc cgattgagcc agccagcgac cattcaccat
     5281 gttgacgacg gcgcagaagt tgcagaagaa tgggcagcca taactggagt gatgcggcag
     5341 tgtgcgttct cccatgaacg tgcgccgcag gtagcgcgca gcatcgaccc ggtggtaggg
     5401 ccagtcgggc aaccggttag gatgcggaat cggcggcgtt gggttggaga tgatctggcc
     5461 gcttcccggg cggcgaaaag ccagacccgg cagaccggtc ggatcgtcgc ctcgccgcaa
     5521 cacgtcgacg agcgacttga agaccgcctc gccatgcccg cacacgacgt agtccacgta
     5581 ccctgagcgc aggcatacgt cgaagtgctg tgtcgggaag taaccacccc agacgatggt
     5641 gagatgagga tgccgcgcct tgacaacacg acacagcggt accgcgtcac ccaactgcgg
     5701 accgggcatc actgtgacag ccaggatgtc cgcacctgtt tcgcgaacga cccgatcgac
     5761 ctcggcgacg gtatcgccca ccaggttgcc gtcgacgatc cggtactcgt gctcgccttc
     5821 gagcaaggcg ccgagcgcca gaagagacat gggcagcacc ggcttgcgct gcgcgctgct
     5881 cggcggatta tacaggatga tcatacgcaa ggtccttgcc cgattcgata gatcgtcatc
     5941 acttcgctga gcaggtcgcg tctaagcatc acgtcggcct gaaagccgtt ggctcgaaaa
     6001 gcccgcagga acgcctcgtc atcgccgagc gaagacagga tgacccaagc gcaaccaccc
     6061 ggcgccaggc gcccgcgcag acccgccgcg aatcgttcgg ccacgtcctc agagcgccag
     6121 gcgaagtcag acacgtcccg tggcctccct cgaaagaagg gtgggttgaa caagatggcg
     6181 tcgaaacgca tatcgcatac gggttcgaac aagtcaccct ctcgaacttc tacgcgatcg
     6241 tcaacatggt tgagcagcac attgatccgc gcacagcaca ccgcctccgg attgacgtcg
     6301 acagccacga cgtgagcggc ccattgcgcg gcgtacacgg ccccaatgcc cgatccggtt
     6361 cccatatcga gcaccgtcga gccggaacga atccattcgg agctgagcga cttcgcaaac
     6421 aactcgctgg agcggaacag cgtcgggttg aacacgcctg gcagcacgag aaacggcctg
     6481 ccattcacat actctaaaac taggcggttg tggcggagcc gttgaaacag ccagaagcgc
     6541 agccaaagca ggaatcgcac gaataagcgc ggcatcacgc gcgagcctcg gcatgcctgc
     6601 ccacgatgag cgggaagcgc gccggttcgc gccggacgcg caaccgcgcc cagccgcgcc
     6661 gcaccgccca gcgccagccg aaggacggat gcgcgacctg ccagcatagc cccagccgcg
     6721 cggctaggtc gtccaacctc gcgtaggtca ggtagttctc gtgcggcagc gcatgcgacc
     6781 tgaagccgta gaggcgcatc cagcgcgcct cgcgttcctg caccatccgc tcgccgctcg
     6841 ccgcttcgcg ataaactggc gaatctacga tggccaggca accgtcgcgg cgcaagacac
     6901 gcagcgcctc gcagagggta gtctcataat cgagcgaata gtgcagcgag ccgttgaaca
     6961 cgaccaggtc tgcctgatcg tcgtcaaagg gcaggcgatc gaactcggcc tgcaccggga
     7021 caaaggctgc atcgtaatgg acgtgtgcgc ccaacccgtc ggtcgagttg atcagaagat
     7081 cgatcgcggc gacgccatga ccgcgccgcg ccaaccggtg cgacagccag ccattgcctg
     7141 cacccaaatc gagggccgtc agcggacgtc gccacgtcca ttccagcggc ttcaccaccc
     7201 gctcgacgag cgcctgaaaa ctcttggcgc ggatacgcca caagtccgcg aaccggccac
     7261 tcacatcgtt aaaaggcaac gcccgatagt aaccggcatc gggggaaccc cagccttcgt
     7321 cgcgtcgcac cgtctcgtac tcgcgcgcga actgtgcgag agcagacgcc cgctgcggcg
     7381 gcaggaggcg ccagatgcca tcgacccgga agtaagcctc ggcatcgttc gggcagcgca
     7441 gggcgtcggg cgactcgcgg accaagcgcg cccggcagac ggggcacgca aaggtcagtc
     7501 gatcgcgatc gatttgcccc acgtgggcgt cacgcatcag tgcgttggaa ttcgagaatg
     7561 acatgatcgg cgagacgatt gaatggaaag acggtcgcag tcgcccattc cagccgagcc
     7621 aataggtcga gcaaccgagg ccggcgcgcc acgaccggct caagatacga cggcggcaga
     7681 aagacgccca ggcccgtcag ctgccggagc atgaaacgcg gcgcgagcac tcgtcgaatc
     7741 gacgcaaccg atgggtacca gacggggacc gtcgcttccc ccactctggc ctcgacgcta
     7801 cgccgcaagc gtcgaaacgc gactcgcggc tgaccgtggg tcacatgcca tgcgatctcc
     7861 caaccacacc accgattgat gaacaccacg acgagccatc cacccggccg cagccacgcc
     7921 gccaaggcgt ttgccaccgg gtgcaaatcg ggaacgcagt tgagcacgcc gaagttggac
     7981 accgccattc caaattcccc ctccgagaat acggcggcag gcgacgcggc atccagtatt
     8041 cgggtgtcga ttctatcagc cacgccggcg cgctcggcct tccggcgggc aacttccagc
     8101 atagcaggtg agatgtctgt cgccacgaca cgagcgcctc gctctgccag ccagaccgca
     8161 tcttcgcccg tgccgcagcc gatctcgagc acgtgcatgc ccgatcggat gtgcacgacc
     8221 aggcgctccc ataccctgct gcgcagcacc cgcgccaact gcgtatcggt gaactgccga
     8281 tcgtagtcga acgcaacttg atcgaatgca gatggctgat ggccgatggc cgatggcgta
     8341 tcgcgtatcg cgtctcgcat atcacttgtc gcatcacaca tcacgtttca ctcgttacga
     8401 ttcacgcatc acgtgctacg tctcacgttc ccccgccgtc agcatcatcc cggcgcggcc
     8461 cagtgcaacg ttcgccgctg ccttcgccag gcgggtcatg ctcgcgccat tcttgcgctc
     8521 gcgatgcagc gccaccgagt tgaccatcca ccgtgtggca aacgaataga agcgcatcga
     8581 gtggcggccc ttcacggtta actgccggtc ggtgcgccga tcccaatcgg cacgagccaa
     8641 cacacggtct tccactttct cgtagtaggc cgtgcctttg atcggatagg ccaccgtcgt
     8701 gaggaacaca tcgggcgtgg acttcttgag gtgatcgacc gtgacctcca ggtcctcgat
     8761 ctcttcgccc tcgtagccga gcatgatgaa catgccggtc tggatgccgc gccgcttcag
     8821 ggcatggatc atcgcctgca cctgctcgac cttcacctca cgccgcattt catcgaggac
     8881 tttctgcgat ccggactcgg aacctatcca caggcgatag cagcccatct cggccagcgt
     8941 gtcgatcacc tgctcgttca agcgatcggc gcgcgagatg cactcgaatg gcgtccgaat
     9001 accgcgccgc ttcagctcgg cagcgtactc gaagaaccag ccatgcttga tcgtgaagac
     9061 gtcgtcggcg taccacaact gatcgggctt gtagcgggac aggagatctt ctacctcgtc
     9121 ggcgacgcgt cctggcgagc gccggcggtg cgtgtgtccg tacaccgcat ggctgcacca
     9181 ctcgcagtga tacggacagc ctcgcgcgca gatgagcgac acgctgccca tgccgtgatg
     9241 cgcgcgccac gtctcgacat accgctcgat atcgatcgct tcgcggtcgg ggtcagggag
     9301 agagtcgaga tcggcgatgt aagtgcgcgg cagggcgcgc acgatcacgc cgtcgtcgcg
     9361 ccggtaggcg atgccgggaa tcccgtcgag tctgtgcgga ccatctcgcg aagcggccag
     9421 tcgaggcagc agctcctcga gcgccgcctc gccctcgccg gcgacgatca catcggcgcc
     9481 gcgatcgaga tattcttcga cgtagcacgc cggctccggt ccgccgagga tgaccgtcgc
     9541 gccgaggcgc ttgcagacgg cgatcagctt cagcacgttg aacttcgtca tcaggttggt
     9601 atatagccca acgactggcg gccgttctcg ctcgatggtt gcctcgaagt cggccatgct
     9661 cccgaacgtc gtatcgagga cgccgacatg gtaacccacc cgcttgaggt acgccgagat
     9721 gtatagaatg ccgagcggcg gatacggctt catcactttc agttcgtgcg ggtcttc
//
LOCUS       DBNHXB010000438         6273 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3826651, whole genome shotgun sequence.
ACCESSION   DBNHXB010000438 DBNHXB010000000
VERSION     DBNHXB010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3826651"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="AC162G_17240"
     CDS             <1..204
                     /locus_tag="AC162G_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077603.1"
                     /translation="AAWGRAAEATAKILSFFRNADTIILVSHGWFIILLTLYLRKHRL
                     IKRGPFVPKVSFGGMTEYLLRTV"
     gene            242..571
                     /locus_tag="AC162G_17245"
     CDS             242..571
                     /locus_tag="AC162G_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR5077604.1"
                     /translation="MDAIQEAEKLLSELTRAEKAQILQWIVRDLGDAFPAIESRPEVC
                     GGEPCIVRTRIPVWVLVRTRQLGASEADLLRSYPTLRAEDLANAWAYFRTHRDEIEQQ
                     IRENEEA"
     gene            801..1490
                     /locus_tag="AC162G_17250"
     CDS             801..1490
                     /locus_tag="AC162G_17250"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5077605.1"
                     /translation="MNDLINKTPELIREAAKSALGLLALVVLIIAILALAFFTTASES
                     VKVIIFALIFVGAGLFVISVLRLAALERTQRTPLPLPSWQSNDSGSVDQVAALCYRPK
                     DASIELLLVKTDGGRWIFPKGNVSTGEELWRAAQREAFEEAGASGEIGHEPLTTFLHL
                     KQGLKQTGVELRIAAFPLRVKATQPPGEKGRKPKWFSPDEAEKAVSKNRQFKYAEELK
                     RVVRLAVATLK"
     gene            complement(1525..1764)
                     /locus_tag="AC162G_17255"
     CDS             complement(1525..1764)
                     /locus_tag="AC162G_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR5077606.1"
                     /translation="MTCKELVELITDYLEDALPPAERARFEAHLGRCDGCTNYLDQMR
                     RTIALSSRLTEESIAPRAQEELLHVFQDWKTGKQS"
     gene            complement(1787..2509)
                     /locus_tag="AC162G_17260"
     CDS             complement(1787..2509)
                     /locus_tag="AC162G_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5077607.1"
                     /translation="MSVPTHPPDDPSATIEPGAARRGVTGVIASADDLQTVEALRRGD
                     EAAFVRLVEAYQASMLRLAMLFVQDRAVAEEVVQEAWVGVLRGLDRFEGRSSLKTWIF
                     RILTNGAKTRGQREGRSVPFSLLGDVDLDPEGPSVEPERFMPADHPKQPHHWASFPRS
                     WDAVPEERLLAQETLSIARAAIESLPPAQREVITLRDVEGWSSGEVCNVLGISESNQR
                     VLLHRARSKVRRALERYFDEEE"
     gene            2683..3504
                     /locus_tag="AC162G_17265"
     CDS             2683..3504
                     /locus_tag="AC162G_17265"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015120391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HIR5077608.1"
                     /translation="MKGIRDKNVLVTGGSSGIGQAIAVRFAEEGANVAINYRKSADEA
                     EKTHDLVHEAIDQTMDHMKAMGVKHVLVQGDVSRENDILRMFDETVKGLGGLDILINN
                     AGIQTPGDSHEISADSFDRVLGVNLRGAFVCAREAIRHFLAERKPGVIVNVSSVHQII
                     PKPRFLSYSVSKGGMQNLTRSLALEYAGRGIRVNGIGPGATITPINRAWVDDPEKRAM
                     VESHIPMGRAGTADEMAAVTVFLCSDEAAYITGQTLFVDGGLTLYPDFRTAWSSE"
     gene            3595..4197
                     /locus_tag="AC162G_17270"
     CDS             3595..4197
                     /locus_tag="AC162G_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077609.1"
                     /translation="MNRRTMSSKSKAVLAVYLSGLLQGIALIIFPAAGPLLTDPQFHG
                     PSSAQFGVLFTPQIAAAIVASALAARLAARVGMKRILLAGLGFNLAKHSIDWWVTTED
                     LPDPRNRVTLTKDGSIRLSYTPNNTEPHKRLLAKLKHAMEHVEGGMTHFIPQAVYLSK
                     RIPIAGVAHQCGTAHFGADPETSVLRPVRSRRPHRSGNEP"
     gene            4194..6257
                     /locus_tag="AC162G_17275"
     CDS             4194..6257
                     /locus_tag="AC162G_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012629666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HIR5077610.1"
                     /translation="MNMIDFGRGVCGDLAAAEKKEWLATNGIGGYASGTVANLLTRRY
                     HGLLVAALKPPLGRTLLVAKLDETATYDGRAYPLFANRWASGVVEPEGFRHVERFHLE
                     GAIPVWTFALADAILEKRIWMKQGANTTTVQYRFRRGSSSLVLEARALVNYRDYHGAT
                     RGAGWQMRVEPVEHGLRVVAFEGATPFYLLSDRAEAAPGHDWHINFSLSLEQYRGFEG
                     IEDHLSVGEFRAALQPGESLTFVLTAEPEPDLDGEAAYAERVDYERRILSHAPAAPEA
                     FQPLVLAADQFIVDRPTPDDPNGRTVIAGYPWFGDWGRDTMISLPGLTLATGRAGVAR
                     SILRTFARFVDRGMLPNRFPDEGETPEYNTVDATLWYFEAIRAYHAATGDDDLVRELY
                     PVLRDIVDWHVRGTRYNIHVDPDDGLLYAGEPGVQLTWMDAKIGDWVITPRTGKPVEI
                     NALWYNALRIMAEFAKMAGGDSSRRQSASFGKLRTSSGDASYGDLADRVKSSFSRFWY
                     ATGNYCFDVIDGPDGNDASLRPNQLLAVSLLHSPLDAERQRAVVDVCARHLLTSHGLR
                     SLAPNHPDYVGHYGGEAHHRDAAYHQGTVWGWLIGPFVSAHLRVHGDRELAQSYLQPL
                     ARHLADHGLGSISEIFDGDAPFTPRGCIAQAWSVAEVLRVLREIEGKRLRKSQGDIPP
                     GELLG"
BASE COUNT         1203 a         1937 c         1987 g         1146 t
ORIGIN      
        1 gctgcctggg gacgggcagc cgaagccaca gctaagatac tctcgttctt tcgtaacgca
       61 gacactatca tccttgtcag tcatggttgg ttcatcatct tgctcaccct ctatctccgc
      121 aaacacaggt tgattaagcg cggtccattc gtgcctaaag tcagtttcgg cggaatgacg
      181 gaataccttc ttcgaactgt ttgagggagt ataatccgtc gtgaaaggca ggtgtttggg
      241 catggacgca attcaagagg ctgagaagct cctgagtgaa ttgacgcgcg cggaaaaggc
      301 acaaatactt cagtggatcg tacgagacct gggtgatgcc tttcccgcta tcgagagcag
      361 accagaagtg tgcggtgggg aaccgtgcat tgtccgcact cgcattccag tttgggttct
      421 ggtgcgtacc aggcagttag gggcgagtga agcagacttg ttacgttcct atcccacgct
      481 gcgtgcggag gatctggcca atgcctgggc ttactttcgc acccatcgag acgagattga
      541 gcagcaaatt cgcgagaatg aggaagcctg aaaggcgcgg ttatggcatc gttgcctgca
      601 tggtcgatgc ggactttatt ggtttggctc accggattca tgaagcactc gagacgcaag
      661 caaacggccc gttcgttcag gtgttgcgat cattcaatcg gaagcaagta tcctgaagcg
      721 cccggcgcgg gactcgcggc tgcacgccgg gttctgaacg agggcaacac tagccgactg
      781 agcggatgga ggcttaggtc atgaacgatt tgatcaacaa gacaccggaa cttatccgtg
      841 aagcagccaa gagcgcgcta ggcttgctcg ccctggtcgt gctcatcata gcaatcctcg
      901 ccctagcctt tttcaccact gcatcggaga gcgtcaaagt gatcatcttc gctctcattt
      961 tcgtcggcgc cggtcttttt gtcatcagcg tgcttcgcct agcggcgctc gagcgaactc
     1021 aacgcacccc gctcccgctg ccatcctggc agtccaatga ctctggaagt gttgaccaag
     1081 ttgccgctct ctgctatcga ccgaaggatg cttcgattga actgttattg gtcaaaactg
     1141 acgggggacg ttggattttc cccaagggga acgtgtctac tggtgaggaa ctctggcggg
     1201 ctgctcaacg cgaagccttt gaagaggctg gcgcatcggg cgagattggt cacgagccgc
     1261 tgacaacctt tcttcatctc aaacaaggcc ttaaacagac aggcgtggag ttacgcattg
     1321 cggctttccc gctgcgggta aaagcaactc agccgcctgg ggaaaagggg cgcaagccta
     1381 agtggttttc cccggacgag gccgaaaagg ccgtatcgaa gaatcggcag ttcaagtatg
     1441 ctgaagagct caagcgcgta gtacgcctgg ccgttgcaac cttgaaatag ctttggggga
     1501 gtccgcctgg ggtaatccag gccttcagga ctgtttgccc gtcttccagt cctgaaacac
     1561 gtggagcaat tcctcctgag cgcgcggcgc gatcgattcc tcggtcaatc ggctggacag
     1621 ggcgatcgtg cgccgcatct gatcgaggta gttggtgcag ccgtcgcatc gccccaggtg
     1681 cgcctcgaag cgggcgcgct cggcgggggg tagcgcgtcc tcgagataat cggtgatgag
     1741 ttcgacgagt tccttgcacg tcatctcacg ggcagccctt atcgcgtcac tcttcctcgt
     1801 caaaatatcg ctccagcgcc cgccgcacct tggagcgcgc gcgatggagc aggactctct
     1861 gattagactc gctgatcccc aaaacgttac acacctcgcc tgaagaccag ccctcgacgt
     1921 cgcgcagtgt gatcacctcg cgctgagccg gcggcagcga ctcgatggct gcccgggcga
     1981 tcgagagcgt ttcctgtgcg agcagccgct cttccggaac cgcgtcccaa ctgcgcggga
     2041 aagatgccca atggtgaggc tgtttcgggt gatccgccgg catgaagcgc tcaggctcga
     2101 cggacgggcc ttccgggtcg agatcgacgt cgccgagcag cgagaacggc acgctgcggc
     2161 cttcgcgctg gccgcgcgtc ttggcgccgt tcgtcaggat gcggaaaatc cacgtcttca
     2221 gagaagaacg gccctcgaac cgatccagcc cgcgcaacac gccgacccac gcctcctgaa
     2281 ccacctcttc ggccaccgcc cggtcttgca cgaacagcat ggccaggcgc agcatggatg
     2341 cctggtacgc ttcgacgagc cggacgaagg cggcctcatc gccgcgacgc agcgcctcga
     2401 cggtctgcag atcgtctgca gaagcgatga cgcctgtcac gccgcgccgc gcggcgcccg
     2461 gctcaatcgt tgcggacgga tcgtccggcg gatgcgtcgg tacgctcatg gctgttgggc
     2521 tttaagtcag acgtgcgccg gtttgcggga caggaagaac cccaggacga ttacgccgat
     2581 gtttcaaagc atactcaaga gcgaatacgt tgtcaaactg taacgaaccg gcgcgcgcca
     2641 agacagtatt ggcgagcaga catctcagga ggagaattca ttatgaaggg cattcgagac
     2701 aagaatgtct tagtgaccgg cggcagctcc ggcatcggtc aggccatcgc agtacgcttc
     2761 gccgaggaag gcgctaacgt cgcgatcaac tatcgcaaga gcgccgatga ggcagagaaa
     2821 acgcacgacc tggtgcacga ggcgatcgat cagacgatgg accacatgaa agccatgggc
     2881 gtcaagcacg tgctcgtgca gggggacgtg tcgagagaga acgacatcct ccgcatgttc
     2941 gacgaaaccg tcaaagggtt gggcgggctg gatatcctga tcaacaacgc cggcatccag
     3001 acgccgggcg attcgcacga gatatcggcc gacagcttcg accgcgtgct cggcgtcaac
     3061 ctgcgcggcg cgttcgtgtg cgcgcgcgag gcgatcaggc acttcctcgc agagcgcaag
     3121 ccgggcgtga tcgtcaacgt gtcgagcgtg caccagatca tccccaagcc tcgattcctc
     3181 agctactcgg tcagcaaggg cggcatgcag aacctgacgc gcagcctggc gctcgaatac
     3241 gccgggcgcg ggattcgtgt gaacggcatc gggccgggcg cgacgatcac gccgatcaat
     3301 cgcgcctggg tggacgaccc cgagaagcgc gcgatggtcg agagccacat cccgatgggc
     3361 cgcgccggca cggcggacga gatggcggcg gtgacggtct tcctgtgctc cgacgaggcg
     3421 gcgtacatca ccggccagac gctgttcgtc gacggcggac tgacgctgta ccccgacttc
     3481 cggacggcgt ggtcgtcaga gtagggagtg gggagttaga gttagagatg gctcggttgt
     3541 gcgacacact ttggaagtgc gtcgcacatg gcaaaggctg ctatggatct cgccgtgaac
     3601 cgtcgcacaa tgagtagcaa gagcaaagcc gtcctggctg tgtatctcag cggcctgctg
     3661 cagggcatcg cgctgatcat ttttccggcg gccgggccgc tgctgacgga cccgcagttc
     3721 cacggcccga gcagcgcgca gttcggcgtg ctcttcacgc cgcagatcgc cgccgcgatc
     3781 gtcgcctcgg ccctggcggc gcggctcgcg gcacgtgtcg ggatgaagcg tattttgctg
     3841 gccgggctgg gattcaacct ggcgaagcat tcgatcgact ggtgggtcac gaccgaagac
     3901 ttgcccgatc cgcgcaaccg cgtcacgctg acgaaagacg gcagcatccg gttgagctac
     3961 acaccgaaca atacggagcc gcacaagcgc ctgctggcga agctgaagca tgcgatggag
     4021 cacgtcgagg gcggcatgac gcacttcatc ccgcaggcgg tatacttgtc caagcgcatc
     4081 ccgatcgcgg gcgtggcgca ccagtgcggc acggcacact tcggcgcgga ccccgagacc
     4141 tccgtgctga gacctgtgag gtctcgaaga cctcacaggt ctggcaacga accatgaaca
     4201 tgattgactt cgggcgcggt gtgtgcggcg atctcgccgc tgccgagaag aaagagtggc
     4261 tggcgacgaa cggcattggc ggctacgcgt cgggcaccgt cgccaacctg ctcacgcggc
     4321 gttaccacgg cctgctcgtc gccgcgctca agccgccgct cggccggacg ctgctcgtcg
     4381 cgaaactcga cgagaccgcg acttacgacg ggcgcgcgta tccgctgttc gccaaccgct
     4441 gggcgagcgg cgtcgttgag ccggagggat tccgccacgt cgagcgcttc cacctcgaag
     4501 gtgcaatacc ggtgtggaca ttcgcgctgg cggacgcgat cctggagaag cgtatctgga
     4561 tgaagcaggg cgcgaatacc acgaccgtcc aatatcgttt tcggcggggt tcgtcgtccc
     4621 tcgtgcttga ggcccgtgcg ttggtgaact accgcgacta tcacggcgcg actcgcggcg
     4681 ccggatggca gatgcgcgtc gaacccgtcg agcatggcct gcgcgtcgtc gccttcgagg
     4741 gtgcgacgcc gttctacctg ttgagcgacc gcgccgaggc tgcccctggc cacgactggc
     4801 acatcaactt ctcgctgagc ctcgagcagt atcgcggctt tgagggaatc gaagatcatc
     4861 tatctgttgg cgagttccgc gctgccttgc agcccggcga gtcgctgacg ttcgtcctca
     4921 ccgcggagcc tgaacccgat ctggacggcg aagcggccta cgcagagcgc gtggactacg
     4981 agcggcgcat cctgtcgcac gcgccggcag cgccggaagc gttccaaccg ctggtcctcg
     5041 ccgccgacca gttcatcgtc gaccgcccga ctcccgacga cccgaacggc aggaccgtca
     5101 tcgccggcta cccctggttc ggcgactggg gtcgcgacac gatgatcagc ctgccgggcc
     5161 tgacgctggc gaccggccgg gccggcgtgg cgcgcagcat cctgcgcacc ttcgcccgct
     5221 tcgtcgatcg cggcatgctg ccgaatcgct ttcccgacga aggcgagacg cccgagtaca
     5281 acaccgtcga cgccacactg tggtatttcg aagcgatccg cgcctaccac gccgcgaccg
     5341 gcgacgacga tctcgtccgc gagttgtatc ccgtgctccg ggacatcgtc gactggcacg
     5401 tgcgcggcac gcgatacaat atccacgtcg acccggacga cggcctgtta tacgcgggcg
     5461 agccgggcgt gcaactgacg tggatggacg cgaagatcgg cgattgggtc atcacgccgc
     5521 gcaccggcaa accggtcgag atcaacgcgc tgtggtataa cgcactgcgc atcatggccg
     5581 aatttgcgaa gatggcagga ggcgactcca gtcgccggca gtcggcatcc ttcggcaagc
     5641 tcaggacaag ttctggagat gcctcctacg gcgacctggc cgatcgggtc aaatccagtt
     5701 tctctcgatt ctggtatgcc acgggcaatt attgcttcga cgtgatcgac gggccggatg
     5761 gaaacgacgc atcgctgcgg cccaatcaac tgttggccgt ctcgctgctt cacagcccgc
     5821 tcgacgccga acgccagcgc gccgtcgtcg acgtgtgcgc gcggcatctg ctgacgtcac
     5881 acggcctgcg cagcctcgcg ccgaatcatc ccgattacgt aggccattac ggcggcgaag
     5941 cgcaccatcg cgacgcggcg taccatcagg gcaccgtctg gggctggctg atcggcccct
     6001 tcgtcagcgc ccacctgcgt gtccacggcg accgggagct ggcgcagtcg tacctccagc
     6061 cgttggcgcg gcatctggcc gaccacggcc tgggcagtat tagcgagata ttcgacggcg
     6121 acgcgccctt cacgccgcgc ggctgcatcg cgcaggcgtg gagcgtggcc gaggttctgc
     6181 gcgtcttgcg cgagatcgag gggaaaagac ttcggaagtc tcagggtgac atcccgccag
     6241 gggagctact tggatagaca ggcgccgtcg cct
//
LOCUS       DBNHXB010000439         3189 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_809087, whole genome shotgun sequence.
ACCESSION   DBNHXB010000439 DBNHXB010000000
VERSION     DBNHXB010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_809087"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="AC162G_17280"
     CDS             <1..677
                     /locus_tag="AC162G_17280"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020725856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR5077611.1"
                     /translation="EVRGYAFAPTFSIWTTIEECLNPPVIWEDGRGWYTTEPFSEPEV
                     FEFPEGIGPVECVNVEHEEVLLIPRWVKCGRVTFKYGLGEQFINVLKTLKMLGLDNKE
                     RIKVKGVEVAPRDVLAACLPDPAHLGDRMVGKTCAGTWVTGMKNGKPRQVYLYQVADN
                     QRCMEKYGVQAVVSQTAVNPVIAMDLLASGTWQGKGVLGPEAFDPDPFLAKMREYDCP
                     FGMKEM"
     gene            750..2033
                     /locus_tag="AC162G_17285"
     CDS             750..2033
                     /locus_tag="AC162G_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5077612.1"
                     /translation="MSDELSPFLALQHRNFRLLWAGQGISIAGTMMQSAAVLWHVSLL
                     VPSDQRGLALGLVGLARVVPILLFSLVSGVVADALDRRKVLLLTQSTLALTAFVLALI
                     TFGGLDVVWPIYVLSALTAAVASFDGPARQSFFPSLVPRQHLPNAISLNTIIFQFGSV
                     LGPTLAGLLIAGAGVGWVYLVNAISFLAVIVALLLMRGVPRHHGAEKREVSLRAAAEG
                     LRYVFSEPLIRSSMLLDFFATFFSSATALLPIFVQDILQVGPEAYGLLAAAPSIGALA
                     TSAAMVRLVDRIVHRGRVLLWAVAGYGMATVVFGLSQSFWLTFTCLALTGAADTVSVV
                     LRNLTRQLVTPDHLRGRMTGVNMIFFLGGPQLGELEAGLLAQAFGAPVSVVTGGIGCL
                     IATGWIAAKTPQLRHYRREEPALVAAVPQPADVSL"
     gene            2153..2959
                     /locus_tag="AC162G_17290"
     CDS             2153..2959
                     /locus_tag="AC162G_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HIR5077613.1"
                     /translation="MDLKTLDAALTEHVRPETFPVAVHLAPPGEALPPKTKRPGRDLS
                     VQVTICQGIAYSRRYGWVMAVGADDLSCPLAAAVFGFKPMLDYMATGHACYRMYTQTL
                     EAGAATETAVEKLPYKSFEYVLSAPLHRASFEPDVIVVYGNAAQVMRLTVAALWREGG
                     RLTSSFSGRIDCSDEIIVPLRSQKPEVILPCYGDRVFAQTQDHEMAFSFPFLWAERII
                     EGLNGTHQGGVRYPIPAFLRYTGQFPAHYEKMNELWASGEEPSSPVTIRG"
     gene            2961..>3189
                     /locus_tag="AC162G_17295"
     CDS             2961..>3189
                     /locus_tag="AC162G_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077614.1"
                     /translation="MKLLRVRLLLLVMLAAFLPAGCAGEVGPTQTAVINVPKPDDPSQ
                     VWDVELNLGAASVRVDAEGDGLVSGLVEYNVE"
BASE COUNT          516 a          990 c         1061 g          622 t
ORIGIN      
        1 tggaagtacg cggttacgcg ttcgcaccca cgttctcgat atggacaacg atcgaagaat
       61 gcctcaaccc gccggtgatc tgggaagatg gccgggggtg gtacacgacg gaaccgttct
      121 ccgagccgga ggtgttcgag ttcccggaag ggattggccc ggtggaatgt gtgaacgtcg
      181 agcacgaaga ggtgctgctc atcccgcgct gggtgaaatg cgggcgcgtc acgttcaagt
      241 acgggctggg tgagcaattc atcaacgtgc tcaagacgct caagatgctc ggactcgaca
      301 acaaggagag gatcaaggtc aagggtgtcg aggtcgcgcc gcgcgacgtg ctggcagcgt
      361 gcctgcccga tcctgcccac ctgggcgaca ggatggtcgg caagacgtgc gcaggcacgt
      421 gggtcacggg tatgaagaac ggcaagcccc ggcaggtcta tctctatcaa gttgccgaca
      481 accagcgctg catggagaaa tacggcgtgc aggccgtcgt gtcgcagacg gctgtcaacc
      541 ctgtgatcgc catggacctg ctcgccagcg ggacgtggca gggtaaaggc gtgctcggac
      601 cagaggcatt cgacccggat ccgttcctcg ccaagatgcg cgagtacgat tgcccgttcg
      661 ggatgaagga gatgtaggcg attgttcgcg gcggatgaca catccgccgc gagcggctca
      721 atcgcagtgc cccgtgagaa tgcgcgcaca tgtctgacga attatcccct ttcctcgctc
      781 tgcagcaccg caacttccgc ctgctgtggg ccggtcaggg catctccatc gccggcacga
      841 tgatgcagtc ggcggccgtg ctgtggcacg tgtcgctgct ggtcccgagc gaccagcgcg
      901 gcctggcgct cggcctggtc ggactggcgc gtgtcgtgcc catcctgctc ttctcactgg
      961 tcagcggcgt ggtcgccgac gcgctcgatc gtcgcaaagt gctgctcctg acccagtcca
     1021 cgctggcgct caccgcgttc gtgcttgcgc tcatcacgtt cggcgggctt gacgtggtgt
     1081 ggccgatcta cgtgctctcg gcgctgaccg cggcagtcgc ctcgttcgac gggccggcgc
     1141 gccaatcctt ctttcccagc ctcgtgccgc gccagcattt gccgaatgcg atcagcttga
     1201 acacgatcat cttccagttc ggatcagtgc tcggcccgac gctggccggc ctgctgatcg
     1261 ccggcgcggg cgtcggctgg gtctatctcg tgaacgcaat ttcgttcctg gcggtgatcg
     1321 tcgcgctcct gctcatgcgc ggcgtcccgc gccaccacgg tgcggagaag cgcgaggtca
     1381 gcctgcgcgc cgccgccgag ggcttgcgct acgtgttttc cgagccgctc attcgttctt
     1441 ccatgctgct cgacttcttc gcgacgttct tctcctcggc gaccgcgctg ctgccgattt
     1501 tcgtgcaaga tatcctgcaa gtcggcccgg aagcgtacgg tctcctcgcc gccgcgccat
     1561 cgatcggcgc gctggcgacg agcgcggcga tggtgcgcct ggtcgaccgg atcgtgcatc
     1621 gcggccgcgt gttgctgtgg gcagtcgccg ggtacgggat ggcgaccgtc gtgttcggcc
     1681 tgtcgcagag cttctggttg acgttcacct gcctggcgct caccggcgcg gccgatacgg
     1741 tcagcgtcgt gctgcgcaac ctcacccggc aattggtgac gcccgatcat ctgcgcgggc
     1801 ggatgaccgg cgtgaacatg atcttcttcc tgggcgggcc gcagttaggc gagctggagg
     1861 ccggcctgct tgcgcaggcg ttcggcgcgc cggtctccgt cgtcacgggt gggatcggct
     1921 gcctgattgc caccggctgg attgccgcga agacgccgca actgcgacat tatcggcgcg
     1981 aagagccggc gctggttgcc gcggtacccc agccagccga tgtaagcctg tagcacgcgg
     2041 acggcagcca ggtgacaagg gatctcgagg caatcgacga tgggcaataa acgatacacg
     2101 atatgcgata cgcgatacgc ggtaagcgcg tatgatgacg gcgaggtgac ctatggatct
     2161 caaaactctg gatgctgcgc tcacggagca tgttcgtccc gaaaccttcc cggttgcggt
     2221 gcacctggcg cctcccggcg aggcgctgcc gcccaagacg aagcgccctg gccgtgacct
     2281 gagcgtgcag gtgaccatct gccagggcat cgcgtattcg cgtcgctacg gctgggtgat
     2341 ggccgtgggc gccgacgatc tctcttgccc gctggcggcc gccgtgttcg gcttcaagcc
     2401 gatgctcgac tacatggcga ccgggcacgc ctgttaccgg atgtacacgc agacgctgga
     2461 ggcaggcgct gcgaccgaaa cagcagtcga gaagctgccg tacaagagtt tcgagtacgt
     2521 gctgagcgcg ccgctgcacc gcgcctcctt cgagccggac gtcattgtcg tatatggcaa
     2581 tgcggcgcag gtgatgcggc tgaccgtcgc cgcgctgtgg cgcgagggcg ggcgtctcac
     2641 ttccagcttt tccggacgca tcgactgctc ggacgagatc atcgtccctc tgcgctcgca
     2701 gaagcccgaa gtgatcctcc cctgctacgg cgaccgcgtg ttcgcccaga cgcaggatca
     2761 cgagatggca tttagttttc catttttgtg ggcagagcga atcatcgagg ggctgaatgg
     2821 tacgcatcaa gggggcgtgc gttatccgat cccggcgttt ttgcgctaca ctggccagtt
     2881 tccggcccac tacgagaaga tgaatgagtt gtgggcgagt ggggaagaac cttcttcgcc
     2941 tgtcacgata cgaggttagt atgaaactac ttcgcgtccg attgctgctc ctcgtcatgc
     3001 tcgccgcttt tttgccggcc ggctgcgcgg gcgaggtcgg acccacccag accgccgtca
     3061 tcaacgtgcc gaagccggac gatccctctc aggtgtggga cgtcgagctg aacttgggcg
     3121 cggcctccgt gcgcgtcgat gcggaaggcg atgggctggt gtccgggctg gtggagtaca
     3181 acgtcgagg
//
LOCUS       DBNHXB010000440        14366 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_809187, whole genome shotgun sequence.
ACCESSION   DBNHXB010000440 DBNHXB010000000
VERSION     DBNHXB010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 14366)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14366)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14366
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_809187"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="AC162G_17300"
     CDS             <1..988
                     /locus_tag="AC162G_17300"
                     /inference="COORDINATES: protein motif:HMM:NF015254.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HIR5077615.1"
                     /translation="PQLQREWIVIDGDAGTQCALGDAYRFYYRRGAGDDKLMIYFQLG
                     GACWSGPTCQPFSPVYDKRVSEYELRWYGGIFDFENPDNPVTGYHIVVIPYCTGDVHI
                     GAAEVEYDTIPGDALKVTHYGYTNTMSVLNWVYERFEAPDHILIAGSSAGSMASIYYA
                     PEIMRHYPGARIVQLGDGYVGVAPAGGDALETWNAYANLSDEIAAPADIDPKTFTITD
                     LYGAVADRFPQHTFAQYTHSADVVQMGYLLVTGGDAASWADAKAQILEELLTLDNFRY
                     YVGEGMLHTILPMDEFYTMEVNGVRFRDWFADLIAGVDVPNVSCARGTPICP"
     gene            complement(1101..2252)
                     /locus_tag="AC162G_17305"
     CDS             complement(1101..2252)
                     /locus_tag="AC162G_17305"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HIR5077616.1"
                     /translation="MTRPPHIETLAVHAGQQPDPSTGAVMTPIYQTATYVQDGVGKHK
                     GYEYARTANPTRAALEACLAELEGGRFGLCFSSGMAAIDTLLRLVKPGEHVLAGDDVY
                     GGTYRLFVHILAEYGIQFDFVDMSDLDAVRAGLRPNTRLVWLETPTNPLMKIADLAAL
                     SEIARANKAHHPTLVAVDNTFASPVIQRPLELGADFVVHSTTKYLGGHSDVVGGAIVT
                     SDEAAYERLKFLQNAVGAVPGPVDCFLTLRGIKTLPLRMERHSQNAMRIAEFLADHPA
                     VRKVMYPGLEDHPNHAIAKKQMKMYGGMISFLPIGDEETARRIVERTRIFALAESLGG
                     VESLIELPAPMTHASVAGSPLEIDPSLVRLSVGIEHVDDLIEDLRQALA"
     gene            2428..3588
                     /locus_tag="AC162G_17310"
     CDS             2428..3588
                     /locus_tag="AC162G_17310"
                     /inference="COORDINATES: protein motif:HMM:NF019522.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HIR5077617.1"
                     /translation="MKIDKSRLLLVGVAFLIIVAMAAIGRSARVPIEPLPAGVEETVQ
                     ARVLEVLEEGEIDRGGIAQPYQKLRAVLLDGELAGRAVTIDHGRYSLLSPESLYRAGD
                     RVLVLRSQGPDGGDVFIITDAVRLDAMAWLAIAFVAIIVVVARWQGVRSLLGMLVSLL
                     VITQFILPMILAGGDPVAISVAGSFVVLAVTLYLIYGWTRKTHAAVTGMLFSLVITGL
                     LAALAVGLARLTGQGAEEALFLQASGAAINLRGLLLAGIIIGALGVLDDVTISQASAV
                     TELAAANPNLAPLDVFRRAMNIGRDHIASTVNTLVLAYVGASLPLLLLFSIYPQPFAQ
                     TLNREFIAEEVVRTLVGSLGLVLSVPITTFLASTIFRGETGDAGAAGPGHGH"
     gene            3689..4048
                     /locus_tag="AC162G_17315"
     CDS             3689..4048
                     /locus_tag="AC162G_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077618.1"
                     /translation="MSGPIHFFDTSDETPRPLEDIRITGVHVEVMPDHRRVLVSVDLT
                     PFFEKPSFDVTLLRDGVEERATSVVGAMSPQVQLTLHLPPGDPGGSYVARVDLLNEDA
                     TVLQTESVSFEVPQRVP"
     gene            4098..6050
                     /locus_tag="AC162G_17320"
     CDS             4098..6050
                     /locus_tag="AC162G_17320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR5077619.1"
                     /translation="MEGLIGKTVGRYQITEHLGRGGMAEVYKAFQPSLDRYVAIKIMH
                     AFLADDKDFRTRFEREAKFIATLRHPNIVQVYDFVVENGQYYMVMEFIDGVTLKSMLE
                     MMHERGGWVTLDDAVTIVVSLGSALRYAHERGMLHRDVKPANVMITSDGHVILTDFGI
                     AKILSATNLTASGAMVGTPSYMSPEQGMGQAGDERSDIYSLGVMLYQLAVGRLPYDAD
                     TPLAVVLKHINEPLPVPRALKSDLPEGVERVIVKALAKNPDDRYQTVAEMTAELKRAM
                     GLPADVTPTEQMKDGSAMKLAGATVVGRIGAVTPQPERAASSATVARTGASSTAARPA
                     APATAVPPHAVPEVAPQPVVQPAAAGRPKWLIPVIALVVLALVAGGIAVVASGTQAPP
                     PPPPTAAPSPTLAPSPTEVGPPEGQFTSESVELRDEPGAEARVKGVVGPAFGPFTVQA
                     RTVDSEWLRIEFPDGTDGWVPANAVDMGGVPVDALPIGRPPATLTPAPTATPAPSDTP
                     RPTSTPTPSHTPAPTRPPATRPPATATPPPLETPTQAALVPLGWGFTHDFCTYDGDNY
                     TCNVTIWGTGGDGKYHFAVENPDTGIWEEKTGGQATYLVRSRRCRVRVQQVRIWDESG
                     NHIEPNLTTDPDVLGHLFPGGACTQP"
     gene            6281..7627
                     /locus_tag="AC162G_17325"
     CDS             6281..7627
                     /locus_tag="AC162G_17325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR5077620.1"
                     /translation="MSPLRARRAPPARTPGMTRAVFRLAWPAVVEQVLIAGVDLTDLF
                     IVGHLGAAALTAVGLTGQVVMLSMAFFGSIAVGCMAIVARQTGAGERVEAARTIQQSL
                     LAAVLLGGGAAIVAGVFAPGILQALGAEPGVVELGAPFMRTIALSMPLAAIALVGNAA
                     MRATGDTRTPMYLTGLQLVVNAVLGILLVYGPLQLGVQGSGIATAIARSTTGALVLWL
                     LWRSRRLAANGWRPDARRLKRILSVGLPAGAEQVLLQFALLNLATVIANLGTVTYAAY
                     SVSIRIMSLSFLPGWGFAVAATTLVGQWLGAGAPERARESAYAAFRLALGVMLVMAAV
                     LYFFAEPILRIFTGDPGVIAEAITAIHVGAFAQPSMAASFVFMGALRGAGDTRTTLAI
                     TVTSIWAVRLGVAYLGAQVLHLGLAGAWLGIWADFSVRALFGWLRFRSGRWQAIRV"
     gene            7736..9604
                     /locus_tag="AC162G_17330"
     CDS             7736..9604
                     /locus_tag="AC162G_17330"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077621.1"
                     /translation="MRSLIRLAAFSRPYWLRIVVTTACLIAITALSLAVPRLLQTAID
                     FGLAQGDAAVIAQIGLALIGVGLARAVLNLVQRYNAEWLSHRLAYDVRNALYEHIQRL
                     PFAFHDHSQTGQLLARSTEDVGNVQRFAGFALIDLINVILLFAGILAIMLATDARLTL
                     IALIPFPILIAYTVRFGIRITPYFNRIDQALARISTVLQETLSGMQVVKAFAREPNRR
                     EQFAAGNRELYDSRVTTIRYWGSAFPTMSLMVAASVALLIGVGGQMVIAGELTLGELV
                     AFNSFILLLSQPVQRLGFIVNMMGEATASAVRIFEVLDEPAAIADRPGAVTLPALRGR
                     VDFDRVTFAYRRPPSPVPALGAEASHQQARAALQDVSFVAEPDQVVALIGPTGSGKST
                     IINLIPRFYDVTSGAVRVDGFDVRDATLESLRSQIGIVLQESILFSMSIRDNIAYGRE
                     DATDGEIVAAAMAARAHDFITAFPDGYDTIVGEKGVTLSGGQRQRVAIALAMLMNPRI
                     LILDDSMSSVDTQTEYAIQQALAELMKGRTTFVIAQRLTTVKRADQILVLYQGRIVQR
                     GVHDDLLREGGLYREIYDLQLRDQEALRHDLAWIEEAPDDGRQRRFAFKSRQEAVT"
     gene            9771..11567
                     /locus_tag="AC162G_17335"
     CDS             9771..11567
                     /locus_tag="AC162G_17335"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077622.1"
                     /translation="MSQIAAKRINLHTDDEVLGKAYDARVTQRLLSYLAPQRRAISMA
                     MALMVVGTLADLAGPYLIKVAIDGGVAARDATALWLATLGYLLVNAAWWFATRERIRT
                     MAIAGQSIIFALRAELFDHLQQLALGFYARHSVGRLISRVVNDVGVMREFVTWALVAV
                     ARDALHLVGLVVAMVVLDWRLSLLTFLVLPLMGVATAVWRSRAREAYRWTRRSQSRVT
                     GTLSENIAGVRVVQSFSREERNCRHFAGDINRDNFDANVASARLSSAFFPTVDFIGSL
                     AVGLVVWIGGTQVLGGGLTAGVLVAFVLYIDQFFNPIRDLAQRYNTFQATMAAGERIF
                     EVLDTEAEVVDAPGATEMPRIEGHVVFENVSFAYEDGVPVLGDVSFEVQPGQMVALVG
                     ETGAGKSTLVKLLARFYDATGGRVRIDGIDVRDVTQRSLRRQLGVVLQETFLFSGTVA
                     DNIRFGRVDASDTEVVGAAQAVGAHEFISKMPNGYDTEVEEGGAILSVGQRQLISFAR
                     ALLADPRILILDEATSSVDTQTEKKIQAAMATLLEGRTAFVIAHRLSTIVKADLILVI
                     DGGRVVERGTHHELLAQRGRYYNLYTMAFEER"
     gene            11569..12690
                     /locus_tag="AC162G_17340"
     CDS             11569..12690
                     /locus_tag="AC162G_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077623.1"
                     /translation="MNEQHIRAYFAPNWVFRLYALKRIPFPVFVVGVPALIWVIGALG
                     ALVLGVLALFLANPLAYPVGLGFALAAYGWFAKTFPPLLAKLFPLMDTTPEQYGSIIR
                     RWTDRIANRLWVMIAFSIPLAVLSLGRIITLWNSRELNWIGAAWVRSEQARFFQVYYI
                     AYDTVVGSFLLGSGAVGILGCAILLYELLNLPLKLAYYRGLRLIGNFSTALAGWAFIA
                     FVFVGLAQVLIGPELFSADLTSRATLPTIATSLIASVALLTAFFAPVYFAHRAIVHAK
                     YQRLTGLEQAQHNSYREIEEITAQITAAPSDRRAASLYKQLEQYHTQLETTAKLIAEI
                     EAIPEWPLTWRGAVELVGAAILPLLNSLATSIQSTLFPK"
     gene            12787..13761
                     /locus_tag="AC162G_17345"
     CDS             12787..13761
                     /locus_tag="AC162G_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077624.1"
                     /translation="MLRVPSENMAVMRLCALTDLPFVFAFSPSRWMEQVRYLRMFQQH
                     VTQRKWASLAEVYPRSLIWMYAYLLFTWGRYCHCLPDVLSRPDRCLPAEMFIRYVCEI
                     DRYVDSFDSRALWRTHVIQFKARPQVRSVATELCAGVQQVGLDASACRALINLILAYR
                     REALSTMQHGADATADNLAQVIVDKERTAGKLWYVWSRMLSCLYEIPADVAQDASQAF
                     FNFGMALQIIDDLSDAPADHAVRAENLFLAVAQTCPADWERLQRHFIEQPAPFLDWPW
                     ARRHTPDAYHAALALYRGYLDRLHQDTCNAAEAHNLCVMLDRMRSLGG"
     gene            complement(13926..14324)
                     /locus_tag="AC162G_17350"
     CDS             complement(13926..14324)
                     /locus_tag="AC162G_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077625.1"
                     /translation="MAASSVITAILANVDRPLGSPLLWVALLFWTLLGGATGVWVLRG
                     KTWRMLARTTRLRLAIGCFAISWGSLAAMALPMARTMPGAYVLGPGQALFIWLVVATG
                     FALALIFVASFVASFITDHQRKQDEGDIFP"
BASE COUNT         2398 a         4485 c         4688 g         2795 t
ORIGIN      
        1 tccccaatta caacgtgagt ggatcgtgat cgacggggac gcgggcacgc agtgtgcgct
       61 gggtgacgct taccgcttct actaccggcg cggcgcgggc gacgacaagc tcatgatcta
      121 cttccagctc ggcggcgcgt gctggtccgg cccaacgtgc cagcctttca gtccggtcta
      181 cgacaagcgc gtgagcgagt acgagctgcg ctggtacgga ggcattttcg acttcgagaa
      241 ccccgataac cccgtgaccg gctatcacat cgtcgtgata ccgtattgca caggcgacgt
      301 gcatatcgga gccgcagaag tcgagtacga cacgattccg ggtgatgcgc taaaggtcac
      361 ccactacggc tacaccaaca cgatgtccgt cctgaactgg gtgtacgagc gctttgaggc
      421 accggatcac atcctcatcg ccgggagcag cgccggctcg atggcgtcga tctactacgc
      481 gcccgagatc atgcgccact atcccggcgc gcgcatcgtg cagctcggcg acggctatgt
      541 gggcgtcgcg cccgccggcg gggacgcgct cgaaacctgg aatgcctacg ccaacctgtc
      601 tgatgagatt gcagcgccgg cagacattga ccccaagacg ttcacgataa ccgatctgta
      661 cggcgcagtc gcggatcgct tcccccagca cacgttcgcg cagtataccc actctgcaga
      721 cgtggtgcag atggggtact tgctcgtgac ggggggagac gcggcgtcgt gggctgatgc
      781 gaaggcgcag atcttggaag agctattgac cctcgacaac ttccgctact acgtcggcga
      841 agggatgctg catacgatcc tgccaatgga cgaattctac acgatggagg tgaacggggt
      901 tcgattccgg gactggttcg cggacctgat cgccggcgtg gacgtgccga acgtcagttg
      961 cgctcgaggc acgccgatct gcccgtgagg tgtgactcac caccaagaca cgaaggcacc
     1021 aagagttgta tgtatgccga aatatggctt ggtgtcttgg tgtcttcgtg gtgaaaggcc
     1081 cacagcgtcg ggcgaggcgg tcaggccagc gcctgccgca aatcctcgat cagatcgtcg
     1141 acgtgctcga tgccgacgga caggcgcacg agcgatgggt cgatctcgag cggcgatccg
     1201 gcgactgagg cgtgcgtcat cggcgcgggc aattcgatca ggctctcgac cccgcccagc
     1261 gactcggcca gcgcgaagat tcgcgttcgc tcgacgattc tgcgtgcggt ctcctcgtcg
     1321 ccaatcggca ggaacgagat catgccgcca tacatcttca tctgcttctt cgcgatggcg
     1381 tgattcgggt ggtcttcgag tccgggatac atcacttttc tgaccgcggg atggtcggcc
     1441 aggaattcgg cgatgcgcat cgcgttctgg ctgtgccgct ccattcgcaa aggcagggtc
     1501 ttgatgccgc gcagcgtgag gaagcagtcc accgggccgg gcaccgcgcc gaccgcgttc
     1561 tgcaggaact tcaggcgctc gtaggctgcc tcgtcggagg tgacgatcgc gccgccgacg
     1621 acgtcgctgt gaccgccgag atacttggtc gtggaatgga cgacgaagtc cgcgcccagc
     1681 tccagcggcc gctggatgac cggcgacgcg aacgtgttgt cgacggcgac gagcgtcggg
     1741 tgatgcgcct tgttcgcgcg cgcgatctcg ctcaaggctg ccaggtcagc gatcttcatg
     1801 agggggttcg tcggcgtctc gagccacacg agccgcgtgt tcggccgcag cccggcgcgc
     1861 accgcgtcga ggtcgctcat gtcgacgaag tcgaactgga taccgtactc ggccaggatg
     1921 tgcacgaaca gccggtatgt gccgccgtac acgtcgtcgc cggccaggac gtgctcgccg
     1981 ggcttgacca gcctcagcag cgtatcgatc gccgccatgc cggacgaaaa gcacagccca
     2041 aagcggccgc cctccagctc ggccaggcac gcttcgagcg cggcgcgcgt cgggttggcc
     2101 gtgcgcgcgt attcgtagcc tttgtgcttg ccgacgccgt cctgaacgta ggtggcggtc
     2161 tgatagatcg gcgtcatcac cgcgccggtg ctcggatcgg gctgctgccc ggcgtgcacg
     2221 gcgagcgtct cgatgtgggg tgggcgtgtc atgtacgatc ctcgggagga gtgtgacgcg
     2281 attgtacttg gggtcgctgg ggcggtcaac tgtggagtta gagtgtggag ttagagtagg
     2341 gagttagagt atggagttag agtgtggagt cagggctctg ttcgtcgtcc gtccttcgtc
     2401 tttcgtcaga tggtgcggag gaaaagcgtg aagatcgaca agagtcggct gctgctcgtc
     2461 ggagttgcgt ttctgatcat cgttgcgatg gcagcgatcg gccggtcggc gcgcgtgccg
     2521 atcgagccat tgccggccgg cgtggaagag accgtgcagg cccgcgtgct cgaggtgttg
     2581 gaggagggcg agatcgatcg gggcgggatc gcgcagccgt atcagaagct gcgcgcggtg
     2641 ctgctggacg gcgagctggc aggccgcgcg gtgaccatcg atcacggccg atattcgctg
     2701 ctgtcgcccg agtcgctgta tcgcgccggg gatcgcgtgc tggtgctgcg cagccagggg
     2761 cctgatgggg gcgatgtgtt catcatcacc gacgcggtgc gcctcgacgc gatggcgtgg
     2821 ctggcgatcg cgttcgtcgc gatcatcgtc gtcgtcgcgc gctggcaggg cgtgcgctcg
     2881 ctgctcggca tgctcgtcag cctgctggtc attacgcagt tcatcttacc gatgattctc
     2941 gctgggggcg atccggtcgc gatcagcgtc gccggctcgt tcgtcgtgct cgcggtgacg
     3001 ctttatctga tctacggttg gacgcgcaag acgcacgccg ccgtgacggg catgctgttc
     3061 agcctggtca tcaccggcct gctggcggct ctcgccgtcg gcctggcgcg gctgacaggg
     3121 cagggggctg aggaggcgct ctttttgcag gcatccggcg cggcgatcaa cctgcgtggg
     3181 ctgctgctgg ccggcatcat catcggcgcg ctcggcgtgc tggacgacgt cacaatcagc
     3241 caggcgtctg ccgtcaccga gctggcggcg gccaacccga atctcgcgcc gctcgacgtc
     3301 ttccgccggg cgatgaatat cggccgcgat cacatcgcct cgacggtcaa cacgctcgtg
     3361 ctggcatacg tcggcgcgtc gctgccactg ctcctcttgt tctcgatcta cccgcagcct
     3421 ttcgcccaga cgctcaaccg cgagttcatc gccgaggagg tcgtgcgcac gctcgtcggc
     3481 agcctgggcc tggtgctgtc ggtgccgatc acgacgtttc tggcaagcac gatcttcaga
     3541 ggcgagacgg gcgacgcagg cgcagccggg ccggggcacg ggcattagcc gaatgtacaa
     3601 atcattcgca aactgtaaca cggtctttac ttgtcgctcg tcatagcgca gtgtataatc
     3661 ttgtttgaat tgcgttgagg tcagtatcat gtccggcccc attcacttct tcgatacctc
     3721 cgatgaaacg ccccgcccgc tggaagacat tcgcatcacc ggcgttcacg tcgaggtgat
     3781 gccggaccac cggcgggttc tcgtctcggt cgacctgacg ccgttcttcg agaagcccag
     3841 tttcgacgtg acgctgctgc gcgacggcgt cgaggagcgc gcaacgtcgg tcgtcggcgc
     3901 gatgtctcca caggtgcagc tcacgttaca cctgccgccg ggcgatcccg gcggctctta
     3961 tgtcgcacgc gtcgatctgc tgaacgagga cgccactgtc ctgcagaccg aaagcgtttc
     4021 cttcgaagtg ccgcaacgtg tcccgtgagc gccgtttgaa ctttgcaatt tgaagtttgg
     4081 agtttgaagg ttttccggtg gaaggcttaa tcggaaagac cgtcgggcgt tatcaaatca
     4141 ccgagcatct gggccgcggc ggcatggccg aggtgtataa ggcgtttcag cccagtctcg
     4201 atcgctacgt ggcgatcaag atcatgcacg ccttcctcgc cgacgacaag gatttccgga
     4261 cgcgcttcga gcgcgaagcc aagttcatcg ccacgctgcg ccatccgaat atcgtccagg
     4321 tatacgattt cgtcgtcgag aacggccagt attacatggt catggagttc atcgacggcg
     4381 tcacgctcaa gtcgatgctc gagatgatgc acgagcgggg cgggtgggtg acgctcgacg
     4441 atgcagtcac gatcgtggtg tcgctcggct cggcgctccg ctatgcccac gagcggggca
     4501 tgctgcatcg ggacgtgaag ccggccaacg tgatgatcac cagcgacggc catgtcatcc
     4561 tgaccgattt tggcatcgcc aagatcctga gcgccaccaa cctgaccgcc tccggcgcga
     4621 tggtcggcac gccgtcgtac atgtcgcccg agcagggcat gggccaggcg ggcgacgagc
     4681 gcagcgacat ctactcgctc ggcgtgatgc tgtaccagct cgccgtcggg cggctgccgt
     4741 acgatgccga tacgcctctg gccgtcgtcc tcaagcacat caacgagccg ctgccggttc
     4801 cacgcgcgct caagtcggac ctgcccgagg gcgtcgagcg cgtcatcgtc aaggcgctgg
     4861 ctaagaaccc ggacgatcga taccagacgg tcgccgagat gacggccgag ctgaagcgcg
     4921 cgatgggcct cccggccgac gtcacgccga ccgagcagat gaaggatggc tcggccatga
     4981 aactggcggg cgcgaccgtc gtcggccgta tcggcgcggt gacgccgcag ccggagcggg
     5041 cggcatcctc tgcgaccgtc gcgcgcacgg gtgccagctc gaccgccgcg cggccggcgg
     5101 cgccggccac agccgtccca ccgcacgctg tgcctgaggt cgccccgcag ccggtcgttc
     5161 aaccggctgc cgccgggcga cccaaatggc tgattcccgt tatcgcgctt gtcgtgctgg
     5221 cgctcgtggc gggcgggatc gccgtggtcg catccggcac gcaagcgccg ccgccgccgc
     5281 cgccgacggc ggcgccgtcg cccacgcttg caccgagtcc gacggaggtc ggaccgcccg
     5341 aaggccaatt cacgtctgaa agcgtcgaac tgcgtgacga gcctggcgct gaggcgcgcg
     5401 tcaaaggtgt cgtcggtcct gcgttcggtc cgttcacggt acaagctcga acggtcgatt
     5461 ccgaatggct gaggatcgag ttcccggatg gcacggatgg ctgggtgccg gcgaatgccg
     5521 tcgatatggg aggtgtgccg gtcgacgcgc tcccgatcgg cagaccgccc gcgacgctga
     5581 cgccggcgcc gactgcgaca cccgcgccga gcgacacgcc caggccgacg tctaccccga
     5641 caccgagtca tacgccggcc ccgactcggc cgcccgcaac gcgcccacct gcgacggcga
     5701 cgccgccgcc gttggagacg cctacgcagg ctgcgctggt cccgcttggc tggggtttca
     5761 cccacgactt ctgcacttat gatggcgaca actacacttg caacgtgacg atctggggca
     5821 ccggtggcga tgggaagtat cacttcgcgg tcgagaaccc ggataccggc atctgggagg
     5881 agaagacggg cggccaggcg acctacctgg tgcgttcgcg gcggtgccgg gtcagggtgc
     5941 agcaggtgcg catctgggac gagtccggca atcacatcga gccgaacctc acgacggatc
     6001 cggatgtcct cggtcatctc ttccccggag gcgcgtgtac ccagccgtag ccggccagta
     6061 gacctggcag gtcttcaaga cctgccaggt ctatcttttc aatcgcgtga tcgcgtgcta
     6121 tgatacgcgc cgctggcgaa cggcagaagg gaggttttca tacccgccca aaagaaggcg
     6181 gctagtgaaa gccgccctac gtcgcgccaa atgtgaaagg cagcgcgtga acacatccag
     6241 gcgaacctct cgcgcccccg gcccacaggc cggggctttg ttgtctcccc tccgggcgcg
     6301 ccgcgcgccg cccgcccgca caccgggcat gacgcgcgcc gtattccggc tagcctggcc
     6361 ggccgtggtc gagcaggttc tcatcgccgg cgtggatctg acggacctgt tcatcgtcgg
     6421 ccatctgggt gcggccgcgc tcaccgccgt cggcctgacc ggacaggtcg tcatgctgag
     6481 tatggcgttc ttcggctcga ttgccgtggg ctgcatggcg atcgtcgctc ggcagacggg
     6541 cgcgggcgag cgcgtcgaag ccgcgcgcac gattcagcag tcgctattgg cggctgtgct
     6601 tttgggcggc ggcgcggcga tcgtcgcagg tgtgttcgcg ccgggcatcc tgcaagcgct
     6661 cggagcagag ccaggcgtgg tcgagctggg cgcgcccttc atgcggacga tcgcgctgtc
     6721 catgccgctg gctgcaatcg cgctcgtcgg caacgcggcc atgcgcgcga cgggcgatac
     6781 acgcacgccg atgtatctga ccgggctaca gctcgtcgtc aacgccgtcc tcggcatcct
     6841 gctcgtctac ggcccgctgc aactgggcgt tcaaggctct gggattgcga cggccatcgc
     6901 gcgcagcacg acgggcgcgc tggtgctgtg gctgttgtgg cgcagtcgcc gcctggccgc
     6961 gaacgggtgg agaccggacg cgcgccgcct gaagcgtatc ctgagtgtcg gtttgccggc
     7021 gggcgcggag caagtcttgc tccagtttgc gttgttgaat ctggcgactg tcatcgcgaa
     7081 cctgggcacg gtgacgtatg ccgcctacag cgtgagtatc cggatcatgt ctttgtcgtt
     7141 cctgccgggg tggggcttcg ctgtcgccgc gacgaccctg gtcgggcagt ggcttggcgc
     7201 gggcgcgccc gaacgcgcac gcgagagtgc atatgcggcc ttccggttgg cgctcggcgt
     7261 catgctcgtg atggcggcgg tgctatactt cttcgccgag ccgatcttgc gcatcttcac
     7321 cggcgatccg ggcgtgattg ccgaagccat caccgcgatc cacgtcggcg cgttcgcgca
     7381 gccttcgatg gcagcgtcct tcgtcttcat gggcgcgctg cgcggcgcgg gcgatacacg
     7441 cacgacgctc gccatcaccg tcacgtcgat ctgggccgtg cggctgggcg tggcctacct
     7501 gggtgcgcaa gtcctgcacc tgggattggc gggcgcgtgg ctcggcatct gggccgattt
     7561 cagcgtgcgc gcgctatttg gctggctccg atttcgcagc ggtcgctggc aggcgattcg
     7621 cgtgtgaccc agtatgtcgg acgcagataa gcgcagattt cgcggatttt gatttagtct
     7681 gcgtccaaaa accctcgcga tcgacgttca tgttgtagag gattacttcc ttaatatgcg
     7741 ctccctgatt cggcttgcag cgttctctcg accgtactgg cttcgtatcg tcgtgacgac
     7801 ggcctgcctg atcgcgatta cggcgctcag cctggccgtg ccgcgcctgc tgcagacggc
     7861 gatcgacttc ggcctggcgc agggagacgc cgccgtcatc gcgcagatcg gcctggcctt
     7921 gatcggcgtc gggcttgctc gcgcagtgct gaacctcgtc cagcggtaca atgccgagtg
     7981 gctcagccac cggctggcct acgacgtgcg caacgctcta tacgagcaca tccagcggct
     8041 gccgttcgcc ttccacgatc attcgcagac gggacagctc ctggcgcgca gcaccgaaga
     8101 cgtgggcaac gtccagcgct tcgccggctt cgctttgatc gatctgatca atgtgatcct
     8161 cttgttcgcc ggcatcctcg cgatcatgct ggcgacggat gcgcgcctga cgctgattgc
     8221 cttgatcccg ttcccgatcc tgatcgccta cacggtgcgt ttcggcattc gcatcacgcc
     8281 gtatttcaac cgcatcgatc aggccctcgc ccgcatcagc accgtccttc aggagacctt
     8341 gagtgggatg caggtggtga aagctttcgc gcgcgagccg aaccggcgcg agcagttcgc
     8401 ggcgggcaac cgcgagttgt atgacagccg cgtcacgacg atccgctact ggggcagcgc
     8461 gtttcccacg atgtcgctta tggtcgcggc atcggtcgcg ctgctcatcg gcgtcggcgg
     8521 ccagatggtg atcgccggcg agctgacgct gggcgagctg gtcgcgttca actcgttcat
     8581 cctgctgctg tcgcagcccg tgcagcgcct gggcttcatc gtgaacatga tgggcgaggc
     8641 gacggcctcg gccgtgcgca tcttcgaggt gctcgacgag ccggccgcca tcgcggatcg
     8701 gccgggcgcg gtgacgctgc cggcgctgcg aggccgggtc gacttcgatc gcgtcacgtt
     8761 tgcctaccgg cggccaccgt ctcccgtccc ggcgctgggg gcggaggcaa gccatcagca
     8821 ggcacgtgcg gccctgcagg atgtctcttt cgtcgccgag ccggaccagg tcgtcgcgct
     8881 gatcggcccg accggctcgg gaaaatcgac gatcatcaac ctgatcccgc gcttctacga
     8941 cgtcacgtct ggcgcggtgc gcgtcgacgg gttcgacgtt cgggatgcga cgctcgaatc
     9001 gctccgctcg cagatcggca tcgtgctgca ggaatcgatc ctgttctcga tgtcgatccg
     9061 ggacaacatt gcctacggac gagaggatgc gacagacggc gagatcgtcg ccgctgcgat
     9121 ggcggcccgg gcgcacgatt tcatcacggc gttccccgac ggctacgaca cgatcgtcgg
     9181 cgagaaaggc gtcacgctgt caggtggaca gcgccagcgc gtagccattg cactagcgat
     9241 gctcatgaac ccgcgcatcc tgattctcga cgactcgatg tcgtcggtcg atacgcagac
     9301 ggagtacgcg atccagcagg cgctcgccga gctgatgaaa gggcgcacga cgttcgtcat
     9361 cgcccagcgg ttgacgacgg tgaagcgcgc cgaccagatc ctggtgctct accaggggcg
     9421 catcgtgcag cgtggtgtgc acgacgatct gctgcgcgaa ggcggcctgt atcgtgagat
     9481 ctacgacctg caattgcgcg accaggaagc gctgcgccac gacctggcgt ggatcgagga
     9541 agcgccggac gatggccggc agagacgctt cgcattcaag tcgcgacaag aagcagtgac
     9601 gtgatgcgtg atacgtgttg cgtggtgcgt gttgcgtgat gcgtgataag taatgcgtga
     9661 taacgtgatg cagtgaatac gtgatccgcg ataagtcatg tgtgaccggc gacgtgtgat
     9721 aagcgctaca cgatacgcga tatgcgatag gcgataggcg ataggccagc atgagccaga
     9781 ttgcagcaaa gcggattaac ctgcatacag acgacgaggt gctcggcaag gcgtacgatg
     9841 cgcgcgtgac gcagcgcctg ttgagctatc tcgctccgca gcgccgcgcc atctcgatgg
     9901 cgatggcgct gatggtcgtc gggacgctgg ccgatctggc cgggccgtac ctgatcaaag
     9961 tcgccatcga cggaggcgtc gcggccaggg acgcgactgc cttgtggctg gcgacgctgg
    10021 gctacctgct cgtcaatgcc gcctggtggt tcgccacgcg ggagcgcatc cgcaccatgg
    10081 cgatcgccgg ccagtcgatc atcttcgctt tgcgcgccga gctgttcgac catctgcagc
    10141 agttggcgct cggtttctat gcgcgccatt cggtcggccg cctgatctcg cgcgtcgtca
    10201 acgacgtggg cgtgatgcgc gagttcgtca cctgggcgct ggtcgctgtc gcgcgcgacg
    10261 cgctccatct cgtggggctg gtcgttgcga tggtcgtgct ggactggcgg ttgagcctgc
    10321 tgaccttcct ggtgctgccg ctcatgggcg tcgcgacggc ggtgtggcgc tctcgcgcgc
    10381 gcgaagccta tcgctggacg cgccgctcgc aaagccgcgt caccggcacg ctctcggaaa
    10441 acatcgccgg cgtgcgggtc gtgcagtcgt tctcgcgcga ggaacgcaac tgccgtcatt
    10501 tcgccggcga catcaatcgc gacaacttcg atgcgaacgt cgcttcggcg cggctgtcgt
    10561 ccgcgttctt cccgacggtc gacttcatcg gctcgctggc cgtggggctg gtcgtgtgga
    10621 tcggcgggac gcaggtcctc ggcgggggac tcacggcggg cgtgctcgtc gcgttcgtcc
    10681 tgtacatcga tcaattcttc aatccgattc gcgacctggc gcagcgctat aacacctttc
    10741 aggcgacgat ggccgccggc gagcgcatct tcgaagtgct ggataccgaa gcagaggtcg
    10801 tcgatgcgcc cggcgcgacc gagatgcccc gcatcgaagg gcatgtcgtg ttcgaaaacg
    10861 tcagcttcgc ctacgaggat ggcgtgcccg tgctgggcga cgtgtcgttc gaggtacagc
    10921 ctggccagat ggtcgcgctc gtcggcgaaa cgggcgcggg caaatcgacg ctcgtcaagc
    10981 tgctcgcgcg cttctacgac gcgaccggcg gccgcgtgcg catcgacggg atcgacgtgc
    11041 gggacgtgac gcagcgctcg ctgcggcggc agctcggcgt cgtgctgcag gagacgtttc
    11101 tgttcagcgg cacggtcgcg gacaacatcc gcttcggccg cgtcgacgcg agcgacaccg
    11161 aggtcgtcgg ggcggcgcag gccgtcggcg cgcacgagtt catctcgaaa atgccgaacg
    11221 gttacgacac cgaagtcgaa gagggtgggg cgatcctgtc ggtggggcag cggcagttga
    11281 tctcgttcgc acgcgcgctg ctcgccgatc cgcgcatcct gatcctcgac gaagccacgt
    11341 cgagcgtcga cacgcagaca gagaagaaaa tccaggcggc gatggcgacg ctgctcgaag
    11401 ggcggacggc gttcgtcatc gcgcatcgcc tgagcacgat cgtcaaggct gacctgatcc
    11461 tggtcatcga cggtgggcgt gtcgtggagc gtggcacgca ccacgagctg ctcgcgcagc
    11521 gcgggcggta ttacaatctg tatacgatgg catttgagga gaggtaagat gaacgaacag
    11581 cacattcgcg cttattttgc gcccaactgg gtgtttcggc tttacgccct gaagcggatc
    11641 ccctttcccg tgtttgttgt cggcgtgccg gccttgatat gggtcattgg tgctcttggc
    11701 gcactcgtcc tcggcgtact cgcgctattt ctcgccaatc cactcgccta tccggtgggg
    11761 ctgggtttcg cgctggcggc ctacggctgg tttgccaaga cgtttccgcc tctgctggcc
    11821 aaactctttc cattgatgga caccacaccc gagcaatatg gcagcatcat cagacgctgg
    11881 accgatcgca ttgccaatcg gttgtgggtg atgattgcct tcagcattcc gctggccgtg
    11941 ctaagcctcg ggcgcatcat cacattgtgg aactctcggg aactgaactg gatcggcgcc
    12001 gcctgggtca ggagcgagca ggcgcgcttc ttccaggtct actatatcgc ctatgatact
    12061 gtcgtcggct cgttcctgtt gggcagcggc gcggtgggta ttttgggctg tgccattctg
    12121 ttgtatgaac tgctcaatct tccattaaag ctggcttatt atcgcgggct gcgcctgatc
    12181 ggcaacttca gcactgccct ggcaggttgg gcgttcatcg ccttcgtgtt cgtgggtctt
    12241 gcccaagtgc tgatcggacc ggaactattc agcgccgatc ttacctcgcg cgccacactg
    12301 ccgacgattg ccacatcttt gattgcgtcc gtcgcgctgc tcaccgcttt ctttgcgccc
    12361 gtctatttcg cacaccgcgc gattgtgcat gccaagtacc agcgactcac cggtctcgaa
    12421 caagcccaac acaattcgta ccgcgagatc gaggagatca ccgcgcagat tacggcggcc
    12481 ccctcagatc gccgtgcggc ctcgttgtat aagcagctgg agcagtacca cactcagctg
    12541 gagaccacag ccaagctcat tgccgagatc gaagcgatcc ctgaatggcc tctgacctgg
    12601 cgcggcgccg tcgagctcgt cggggcggcc atcctcccgc tattaaattc gctcgccact
    12661 tccattcaat caacgttgtt tccgaagtag ctccccgttc cgcgatcgca tctgagtccg
    12721 acatatttcg ccctatggga ggtgaatatg ctaggcgcac ttgtccatcg agttctgcag
    12781 acaaggatgc ttcgcgttcc ttcagagaac atggccgtaa tgcgcctgtg tgcactgacc
    12841 gatctaccgt ttgtgttcgc gttttcaccc tcgcgctgga tggaacaagt tcgctacctg
    12901 cggatgtttc agcagcacgt gacgcagcga aagtgggcca gcctggcaga agtctatccg
    12961 cgcagtctga tctggatgta tgcctaccta ttattcacct gggggcgcta ctgtcactgc
    13021 ctgccggatg ttctctcgcg gcccgatcgc tgtttgccgg ccgaaatgtt catccgctac
    13081 gtgtgcgaga tcgatcggta tgtggacagc tttgacagcc gtgcgttgtg gcgcacacat
    13141 gtaatacagt tcaaagcgcg tccgcaagtg cgcagtgtgg caaccgagtt atgcgccgga
    13201 gtgcagcaag tgggattgga cgcgtcagcc tgccgtgcct tgatcaacct gatcctcgct
    13261 taccggcgtg aggcgctcag cacgatgcag catggtgctg acgccacagc cgataacctt
    13321 gctcaagtga ttgtcgacaa agagcgaacg gcggggaagc tatggtatgt gtggagccgg
    13381 atgctgagtt gtctatacga gatacccgcc gatgtagctc aagacgcatc acaggccttc
    13441 ttcaatttcg gaatggcctt gcagatcatc gacgatctca gcgacgcacc agccgatcac
    13501 gccgtgcgag ccgagaatct tttcctcgcc gttgcgcaga cttgtccagc cgactgggaa
    13561 cggcttcagc gtcacttcat tgagcagccg gccccctttt tggactggcc ctgggcgcgc
    13621 cgtcacacgc ccgacgctta tcacgcagcg ctcgctctgt atcgaggtta cctcgatcgc
    13681 ctgcaccagg acacctgcaa cgccgcagaa gcacacaatc tgtgtgtcat gctcgatcgg
    13741 atgcgctcgt taggaggttg atcaccactc agatgcgagt ctactactgt cgtggcaggg
    13801 ctgctgccgt gtttgagctt gaaaagcgca gattcactct agcacgaagc gaagtcctgc
    13861 cttgcgatcg tgtgttagca cgcggagcgc cgaacattca tggagtgtcg gcgtttcatt
    13921 ggtttttatg ggaagatgtc gccctcgtct tgcttccgct gatggtcggt gatgaacgag
    13981 gcgacgaagg aagcgacaaa gatcagcgcg agcgcgaatc ctgttgcaac aactagccaa
    14041 atgaagagcg cctgacccgg ccccagcaca taggcgccgg gcatcgtcct ggccataggc
    14101 agcgccatgg cggccaggct tccccacgaa atagcaaagc agccgatcgc caatcgcagc
    14161 cgcgtggtgc gagccagcat tcgccaagtc tttccacgga gcacccagac accggtcgcg
    14221 ccacccagca gtgtccagaa gagcagcgcc acccagagca gtgggctgcc taagggtcga
    14281 tccacgttcg ccaggatggc ggtgatgacg ctgctcgcgg ccaccgctag agggcttgaa
    14341 gcccgaaagt catagcgaat gagtca
//
LOCUS       DBNHXB010000441        15283 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3828488, whole genome shotgun sequence.
ACCESSION   DBNHXB010000441 DBNHXB010000000
VERSION     DBNHXB010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 15283)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15283)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15283
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3828488"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="AC162G_17355"
     CDS             <1..551
                     /locus_tag="AC162G_17355"
                     /inference="COORDINATES: protein motif:HMM:NF017946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077626.1"
                     /translation="TPNVQDFTEHLRLGTMDFILTKPLNSQFHASLRRINVWRLADAL
                     LGAGLIVYATARLDAAPTPDRIAMFILLGVCAAIIMYALVMLLVTSAFWLVQLENVME
                     LLFTFYEAGRFPVTIFPAWMRALLTFVVPIAFVTTVPASVLLGRLNVEFVLYAVGVAA
                     ILFTASVLYWRYAVRHYSSASS"
     gene            complement(530..1237)
                     /locus_tag="AC162G_17360"
     CDS             complement(530..1237)
                     /locus_tag="AC162G_17360"
                     /inference="COORDINATES: protein motif:HMM:NF012345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR5077627.1"
                     /translation="MRVLVFQHDSDDGPGYLGEALTRRGATLHIVRLDAGDGVPDPSG
                     YDLLLILGGEMNVYQEAKYPWLVEENRAIQKALAENKPVLGACLGGQLLAKALGARVH
                     LNAAPEIGLTPIALTEAGKRDPLFAGLSNGLEAVEWHCDTFDIPAGAVALASSAGCAN
                     QAFRFGDRAYGFQFHPEITPAMLAEWIKVTPGDLVDGPAFLNEVEMKAEALRAQAERL
                     IDNFLGRVSRDHELALL"
     gene            complement(1240..2379)
                     /locus_tag="AC162G_17365"
     CDS             complement(1240..2379)
                     /locus_tag="AC162G_17365"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5077628.1"
                     /translation="MPLDLSSIPILDHHCHSLLRHGGPFSAREFQRFFTESSDDVIRA
                     EHVPNTVFFRWAVKELAAFFGCAPAAEAVLAARAAISPDEVAARMFRDANIPLLLMDY
                     GYQGGESYTHDELKTRLPCRVEPVLRLETMAQDLILRHDTFDHVVDAFVAAVEGARAA
                     GYVALKSIIAYRTGLAIRETSPIEAAESFAPVKEQARREGKLRLATKPLNDYLALCAL
                     DVAEKQGLPVQFHTGFGDSDVDLLQANPLLMRPLFQSGKYAHVPFVILHAGYPYVREL
                     SYLASIYSNVFMDISLAIPHIAADIPAMVSQALSLAPTSKVLFATDAYSIPEIFWIAA
                     RWGRWGLAKVLEDLMAAGALHQAEALTAARQILGENAARVYQVEF"
     gene            complement(2387..3769)
                     /locus_tag="AC162G_17370"
     CDS             complement(2387..3769)
                     /locus_tag="AC162G_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR5077629.1"
                     /translation="MPPKRPARAAAPADRVTEAARKAGVRLVRFLYTDNGGVTRGKLT
                     HIEGLPRRLTDGIGLTVAMQAMNMLDQLAPVEGMGPVGEIHLVPDPETFTILPYAPHS
                     AAMTVDMCQLDGTPWPACPRAFLKRQIEACAAAGFSVRATFECEFILATRNADGAYTP
                     LDESLCFSSVGMTTAATVMDDIVAALEAQDIQVDQYYPELGHGQQELTIWHAPALAAA
                     DRQVYYRDTVRNVAFQHGLYASLAPKPFPNQAGNGAHIHFSLWDKAGRRNVMHDLKDP
                     YCLSPLGYHFIAGVLDRLPALLALTCPTYNSYRRLQPHFWSSAYTAWGPDNREGAVRI
                     PSTFKSDRAGSTNAELKASDPSSNPYLALGGLLAAGLDGVKRKLMPGEPALIDPGNYS
                     DEERAARGIRRYPTTLKEALDNLERDQVLLDALGPLLARSFLAVKRLEWESFSKEDEA
                     FETRHHFWKF"
     gene            complement(3857..5248)
                     /locus_tag="AC162G_17375"
     CDS             complement(3857..5248)
                     /locus_tag="AC162G_17375"
                     /inference="COORDINATES: protein motif:HMM:NF012348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR5077630.1"
                     /translation="MPARRTPARKTDPLAALEKQIAADGVEYVRFEQTDTHGISRSKT
                     VPVRHLRYFTEHGLNFLLGQLGFDVQAGVAPNTGYLEELGFPDSRIRPDLSTYRVLPW
                     ADKTARLLCEPYFQDGRPAVAAPRAVARKLLDELEAMGYHLLSGYEYEFYFVSRDGSQ
                     PPYPGIQIFATLRNNFDEALVYQILRGMSGVGVDIITANAEYGPGQMEINFAPALGIA
                     AADAAFTFKNGVKEIAQRSGYMASFMTKPYADQSASGCHFHHSLLNARTGRNAFSNPN
                     RPMELNEACRWWLGGQIAHAAAICALVAPTVNCYKRYKLYSFAPTNATWGFENRTTGV
                     RIKGLKGEGAHVENRTPGGGSNPYLVMAACVASGMDGLKNKIEPPAETPGIAYGLSGV
                     ADLPTRLETALSALEADPALRAALGEEFIKLFVAVKRHEIEKARKGCANYDADDFNQR
                     VDPWEMAEYFEFL"
     gene            complement(5261..6439)
                     /locus_tag="AC162G_17380"
     CDS             complement(5261..6439)
                     /locus_tag="AC162G_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR5077631.1"
                     /translation="MNPIIAFLESARPNFLSDLAALVNVDCGTHNKAGVDAVGEWIGA
                     RCAAWGWEVQRLPQVEYGDCWLARLRGDGVGRLLLMGHLDTIYPDGTAAARPMRLAGA
                     KIVGPGTCDMKGGLLVGMYALRALQTTDFRGFSELAFFFNSEEEVSSPVSQSLYAPIA
                     REMDAVLVLESARANGDIVSARKGSGEYTIRVIGKAAHAGVEPEKGANAILELAHQIM
                     ALHRLNGLAPGVTVNAGVIGGGTKSNVVAAEAWVRVDARAADPAGAEALAQAIADLAG
                     HTTIPRTQVEVAGGFHYPPMAKTPAVALLVEFARNAARDLGFEVNDAATGGASDANII
                     AALGVPVLDGLGPVGGLDHSPDEYIEADSIVPRTALVAGLIQLILSQREGLISVGSHT
                     "
     gene            complement(6478..6714)
                     /locus_tag="AC162G_17385"
                     /pseudo
     CDS             complement(6478..6714)
                     /locus_tag="AC162G_17385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
     gene            complement(6862..8031)
                     /locus_tag="AC162G_17390"
     CDS             complement(6862..8031)
                     /locus_tag="AC162G_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5077632.1"
                     /translation="MKGKLFALAGIASLLLAACGGGAATTGPIKIGGGFALTGAESAL
                     DVPAANGAKLALKEINAAGGVLGRQVELVVHDSQYKMDVTAQIAKQFVEQDQVVAVIG
                     FTDTDSVLASGPTIQAAGLPFITAGATSPKIPTQVGDMMFLACFGDNVQAAVGAEYGF
                     KNYGQTAYLLWDKGVEYTTLLSGYFKARFTELGGTIVLEDSYETDATDFSAQIAKVKA
                     LPQQPDFYFISAMPYNVGPVVKQFRDAGLTGPIVGGDGYDTPDLVSVAGPAAENVFFT
                     THALIDATGGTEGIKKFIAAYKAEYAHDPENAFAALGYDTMYLIADAIKRAGSTDPAA
                     IKKAIEETQNFPGITGSITFSPEAHVPQKGVTVIAVKDGKFTLGAELVPEKVPPP"
     gene            complement(8122..9165)
                     /locus_tag="AC162G_17395"
     CDS             complement(8122..9165)
                     /locus_tag="AC162G_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5077633.1"
                     /translation="MIDIHRVRRAAGPITALLSLVIVLALLQASGLAYWQGLFINLGI
                     FLILVLSLNLSSGFTGVFSLGHIGFMALGAYGSAILTLPLREKQEYLPNLPAGLAGVH
                     FDFHLGPFPLGFLAATLVTALLVSLVALLVGLVLMRLSGHFVAVATLGFLVIVRVILL
                     NADHFTRGSRTFSNVTPYTNLGWVWAWALVALYVVWRIKRSPFGRAMLAQREDRWAAQ
                     SIGIAVLPPRLLAFVVSAFFTAVAGSLYAHFITSFSPTVFYVDLTFRVITMLVVGGMG
                     SVSGSAIGTVLVVGLAEALRRIEDATLLYGMSQIILAVIFILLIIFRRGGLLGQREIA
                     FNRLFMKGGEPSS"
     gene            complement(9162..10136)
                     /locus_tag="AC162G_17400"
     CDS             complement(9162..10136)
                     /locus_tag="AC162G_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5077634.1"
                     /translation="MLVYVTQQLVNALSIGSLYALMAVGLAMVFGILRLINFAHGDLM
                     MVAAYFAAFALFANLPLGLVLLLMIGGTVMVGLLMERVAYRPIRGAPDVAALLTSFAV
                     GQILQNGTLLFTRRIQKPIQIAFPSPEILNGVSALGPITVPRLNIVSLVAGAILLVLL
                     SVFVTRTTLGLSMRAAAEDISAAQLMGININRVIATAFAIGSGLAAVAGLLFAVQAGQ
                     INPYIGFTPVLKAFIAAVIGGFGSISGAVVGGYVLGGLEVFLTAIPGIGDILPAGLVS
                     PEAQAFLKQWLPASLTSYRDAFVFVALILVLLFRPNGILRRRVREEMQ"
     gene            complement(10139..10843)
                     /locus_tag="AC162G_17405"
     CDS             complement(10139..10843)
                     /locus_tag="AC162G_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013144323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077635.1"
                     /translation="MLELHDVEVYYGSIRALKGVSLTVAEGELVTLIGSNGAGKSTTL
                     KTISGLLRPKGGRVQYRGQPIDRLPPHQIVALGISHCPEGRHIFGRLTVAENLRLGAV
                     RRRDPDGIRQDRERVYELFPVLKERSRQLAGTLSGGEQQMLAIGRALMSRPKLLLLDE
                     PSLGLAPLLVAAIFDTIQQLKHAGTTILLVEQNARLALGIADRGYVMETGRIILAETA
                     AELKANPQVEHTYLGT"
     gene            complement(10827..11657)
                     /locus_tag="AC162G_17410"
     CDS             complement(10827..11657)
                     /locus_tag="AC162G_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077636.1"
                     /translation="MKLVPTDSSEPILQAHGLAKSFLGVQALQDFSVAVRPGEILGLI
                     GPNGAGKTTCFNLLTGFLVPTAGRIQLEHQDITGLPPARITRLGIARTFQNIRVFGSL
                     SVLENVQAAAQLRTATSLWETLISGPGFRRQEARVKARALEYLDLFGLIHFAERPAAS
                     LPYAYQRRLEIARALATGPTLLLLDEPAAGMNPAESDALHQLILDLRGRFGLTIVLVE
                     HDMRLVMNLCERIIVLNYGRIIAEGKPEAVRADPQVIAAYLGEDHSNLIGAWVRARTA
                     "
     gene            11873..12298
                     /locus_tag="AC162G_17415"
     CDS             11873..12298
                     /locus_tag="AC162G_17415"
                     /inference="COORDINATES: protein motif:HMM:NF039192.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5678 domain-containing protein"
                     /protein_id="HIR5077637.1"
                     /translation="MARQALREVNAPYTVNSEDEAIGRDTIVIRRSGEPVAVVVPYAE
                     YVELLARPSAQPSEPVSGDPDFERERAAFRRLLPELLKAHRGEWVAIVNEQAVEFGPD
                     FGSVILRVRERFGQRAVYVQEILEKPRVYKFGSPHVIRS"
     gene            12295..12687
                     /locus_tag="AC162G_17420"
     CDS             12295..12687
                     /locus_tag="AC162G_17420"
                     /inference="COORDINATES: protein motif:HMM:NF025341.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HIR5077638.1"
                     /translation="MMIPYDTSLIPPAPFLSAHVSNLTGSAESPPIPAKVDTGADVTA
                     IPANLVNQLNLVLASEIQVEGYNGHRATLPCYDIVLRVAHLRVVGLSVISFSEDYILL
                     GRDVLNLLRLLLDGPALWLEVLSPQSPS"
     gene            12881..13102
                     /locus_tag="AC162G_17425"
     CDS             12881..13102
                     /locus_tag="AC162G_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077639.1"
                     /translation="MQGWRNLNHGDDRIEIYDTLAEARERFGENFQGFVFTLTRAQAE
                     ALLQGKVVAFDIGGREYAGFMLVQEPPGP"
     gene            complement(13348..14574)
                     /locus_tag="AC162G_17430"
     CDS             complement(13348..14574)
                     /locus_tag="AC162G_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077640.1"
                     /translation="MPAEAVTSPRLPHSPPPTSGSVSEALESFQGVQSRALRWALRFL
                     PWLMLLVTTVRLTAFEVGPGNVMYFSGIVAAALSLFAFQVLMQRIPETFRILWDRNTV
                     RARPKRPTDAAHSASVTTEESTPPEEQYRAFIDNVKRWLNDSRQRIMGVIIGLLGAGW
                     IVVDSYRLGGWKALLDPFADPVLIGQFIIEFIIGYIIGLLAWRMIVAGYQIWQMGMKF
                     DLALQLGHPDRCGGLSPIGNLCLWNALIVSTAGVHLGGWIILGPILPEQYSDLAAWYT
                     PLFSTLLLVPLAVAAISFFLPLWSVHQIMVAKRALIQRQLDQLGQSINHLARELLDRA
                     DELDPVESEKMARKLERMQQIYRENQHISVWPFNTRILAKFTTSQIVPLLGLTGLGQP
                     VLKAIAGLLDFLNQAQ"
     gene            complement(14858..>15283)
                     /locus_tag="AC162G_17435"
     CDS             complement(14858..>15283)
                     /locus_tag="AC162G_17435"
                     /inference="COORDINATES: protein motif:HMM:NF023295.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme endonuclease
                     domain-containing protein"
                     /protein_id="HIR5077641.1"
                     /translation="FQRMIEDYNAGATDVDAVFAQLVSFARQLDEEEKRGIAENLSEE
                     ELAIFDILTRPSPKLIKREREKIKQIARDLLDTLKAERLVLEWRSRQQTRAAVRVAIK
                     RALEDLPRAYTDDLYQQKCEAVYQHVYDSYAGPNQSVYA"
BASE COUNT         2934 a         4690 c         4771 g         2888 t
ORIGIN      
        1 tcacgccgaa cgtgcaggac ttcacggagc acctgcgcct gggcacgatg gacttcatcc
       61 tgaccaagcc gctgaattcg cagttccacg cctcactgcg gcgcatcaac gtctggcggc
      121 tggccgatgc gctgctcggc gccggtctga tcgtctacgc cactgcgcgg ctcgacgcag
      181 cgcccacgcc cgatcggatc gcgatgttca tcctcctcgg cgtgtgcgcg gccatcatca
      241 tgtacgcgct cgtcatgctg ctggtcacga gcgcgttctg gctcgtgcaa ctggagaacg
      301 tgatggagct gctgttcacg ttttacgagg cggggcgctt cccggttacg atattcccgg
      361 cgtggatgcg cgcgctgctc accttcgtcg tgccgatcgc cttcgtcacg accgtgccgg
      421 cctcggtgct cttgggtcga ttaaatgtcg agttcgtgtt gtacgcggtg ggggtggcgg
      481 cgattctgtt cacggccagc gtgctgtact ggcgatacgc ggtgcggcat tatagcagcg
      541 cgagctcgtg atcgcgcgac actctgccaa ggaaattgtc aatcagccgc tcggcctgcg
      601 cgcgcagcgc ctcggctttc atctcgactt cattgaggaa cgccggcccg tccaccaggt
      661 cgccaggtgt cactttaatc cattcggcca gcatcgcggg cgtgatttcg gggtgaaatt
      721 ggaatccgta tgcccgatcg ccgaagcgaa aggcttgatt ggcgcagccg gcggaactgg
      781 ccagcgccac ggcgccggct gggatgtcga acgtatcgca atgccactcc acggcctcca
      841 ggccgtttga caacccggca aacagtggat cgcgcttgcc tgcttccgtg agcgcgatcg
      901 gtgtgagacc gatctcaggc gccgcgttca gatggacgcg cgcgccgagc gctttggcca
      961 gcaattgccc gcccaggcaa gcgcccagca ccggcttgtt ctcggccagg gctttctgga
     1021 tcgctctgtt ctcttcgacc agccagggat atttggcttc ctggtagacg ttcatctccc
     1081 cgcccaagat caagagcaag tcatagccgg acgggtcggg caccccgtcg ccagcatcca
     1141 accgaacgat gtgcaacgtg gcgccgcgcc gggtcagcgc ctcgccaagg taaccaggcc
     1201 catcgtctga atcgtgctga aagacgagca cgcgcatgtt cagaattcca cctgatagac
     1261 ccgcgccgcg ttctcgccca aaatttgtcg ggcggcggtg agggcttcgg cctggtggag
     1321 cgcgccagct gccatcaggt cttcgagaac tttggccagg ccccagcgcc cccagcgcgc
     1381 ggcaatccag aaaatctccg ggatgctgta agcatccgtc gcaaacagca ctttgctcgt
     1441 gggggccagg ctgagcgcct ggctcaccat cgccgggatg tcggcggcaa tgtgaggaat
     1501 ggcaagcgag atgtccatga acacgttgga gtagatggag gctaaataac tgagttcccg
     1561 cacgtaagga taaccggcgt gcaggatgac gaagggtaca tgagcatact tgccgctctg
     1621 aaacagggga cgcatcagca gggggttggc ttgcagcaag tccacgtcag aatcgccgaa
     1681 gccggtatgg aactgaaccg ggaggccctg cttttcagcg acatcgagcg cgcacagcgc
     1741 caggtaatcg ttcagcggct tcgtcgccag ccgcagttta ccctcgcgcc gggcctgctc
     1801 tttcacaggc gcgaacgact cggccgcctc gatcggcgac gtttcccgta tggctaggcc
     1861 agtgcggtaa gcgatgatgc tcttgagcgc gacgtagcca gccgcgcgcg ctccttcgac
     1921 cgccgcaaca aaggcatcga ccacgtggtc gaaagtatcg tggcgcagga tgaggtcttg
     1981 tgccattgtc tccagtcgca gcaccggctc aacgcggcat ggcaggcggg ttttcaattc
     2041 atcgtgagtg tagctctcgc cgccctgata gccgtagtcc atcaacagga gggggatatt
     2101 cgcgtcgcgg aacattcgcg cggccacttc gtcaggcgag attgcggctc gggccgccag
     2161 cacggcctcg gccgccggcg cgcagccgaa gaaggccgct agttctttga ccgcccagcg
     2221 gaagaacacg gtattcggaa catgctcggc acgtatgacg tcatcgctgc tttcggtgaa
     2281 gaagcgctgg aactcgcgcg cactaaacgg tcccccgtgg cgcagcagcg agtggcagtg
     2341 gtgatcgagg atagggatgg aggacaggtc gagtggcata gtcttttcag aacttccaga
     2401 agtggtgtct ggtctcgaag gcctcatcct ctttgctgaa tgactcccac tccagccgct
     2461 tgaccgcaag gaacgatcgc gcgagcaatg ggccgagcgc gtcgaggagt acctgatctc
     2521 tttcgaggtt gtcgagcgcc tctttcagtg tcgtcgggta gcgccgaatg ccgcgcgccg
     2581 cgcgttcctc gtcggagtag ttgcccgggt caatcagtgc cggctcaccg ggcatgagct
     2641 tccgcttcac gccgtccagc ccggcggcga gcaatccgcc cagtgccagg tacggattag
     2701 acgagggatc ggaggccttg agttccgcgt tagtcgaacc ggcgcgatcg gacttaaacg
     2761 tagacgggat gcggaccgcg ccttcgcggt tgtccggccc ccaggcggtg taggccgagc
     2821 tccagaagtg cggttggagg cggcggtacg agttgtaggt tgggcaggtg agcgccagca
     2881 gcgcgggcag gcgatcgagc acgccggcaa taaagtggta gccgagcggg ctgagacaat
     2941 aggggtcttt cagatcatgc atcacgttgc gccggccggc cttgtcccac aggctgaagt
     3001 gaatgtgcgc gccattgccc gcctgattgg ggaacggctt cggcgcgagc gaagcataca
     3061 gaccgtgctg gaaggccacg ttgcgcacgg tgtcgcggta atagacttgc cggtcggcgg
     3121 cggcgagtgc gggcgcgtgc cagatcgtaa gttcctgctg gccatgcccg agttcggggt
     3181 aatactgatc gacctggatg tcctgcgctt caagtgcggc gacgatgtcg tccatcaccg
     3241 tggcggccgt cgtcatgccg accgaggaga agcacaggct ctcgtcgagc ggcgtgtacg
     3301 cgccatcggc gtttcgggtg gcgaggatga attcgcactc gaaggtggct cggacgctga
     3361 aacctgcggc ggcacacgcc tcgatctgcc gcttgaggaa cgcgcgcggg caggccggcc
     3421 agggcgttcc atcgagctgg cacatatcca ccgtcatcgc cgccgaatgc ggcgcatacg
     3481 gcagaatcgt gaaagtttcg gggtcgggca cgaggtggat ttcgccgacc ggccccatac
     3541 cctcgacggg ggcgagttga tcgagcatgt tcatcgcttg catcgccacg gtcaggccaa
     3601 tgccgtcggt caggcgcctg ggcaagccct cgatgtgggt caatttgcca cgtgtcacac
     3661 cgccgttgtc ggtgtaaagg aatcgcacca ggcgcacccc ggccttgcga gcggcctcgg
     3721 tcacgcgatc cgcaggcgct gcggcgcggg caggacgttt gggaggcata ggtctctcct
     3781 tgcctcaccc tccccctggc cccctcccgc tccagacgtt acgtcaggag cgggaggggg
     3841 gtgggggtag gtgaggtcat aggaactcaa agtattccgc catttcccac gggtcgacgc
     3901 gctgattgaa atcgtcggcg tcgtagttgg cgcagccctt gcgcgccttc tcgatctcgt
     3961 ggcgcttgac ggcgacgaac agcttgatga attcctcacc caacgcggca cgcagcgccg
     4021 ggtcggcctc gagcgcgctt agcgccgttt caaggcgcgt tggcaaatcg gctacgccgg
     4081 agagtccgta ggcgatgccg ggcgtctcgg cgggcggctc gatcttgttt ttcagcccgt
     4141 ccatcccgga ggcgacgcag gccgccatca ccaggtacgg attcgacccg ccgcccggcg
     4201 tgcggttctc gacgtgcgct ccctcgccct tcaacccttt gatccgcacg ccggtcgtgc
     4261 ggttctcgaa gccccaggtg gcgttcgtcg gcgcgaacga gtagagcttg tagcgcttgt
     4321 agcagttcac cgtcggcgcg acgagcgcgc aaatggctgc cgcgtgcgcg atctggccgc
     4381 ccagccacca acggcatgct tcgttcaatt ccatcgggcg gttgggattg gaaaaggcat
     4441 tccggcccgt cctggcgttc agcaggctgt gatgaaaatg gcagccggag gccgactggt
     4501 cggcgtaagg cttggtcatg aacgaagcca tgtagccgct gcgctgggca atctctttga
     4561 caccgttctt gaaagtgaag gccgcgtcgg ctgccgcgat acccagtgcc ggagcgaagt
     4621 tgatctccat ttgccctggg ccatactcgg cgttggcggt gatgatgtcc acgcccaccc
     4681 cgctcatgcc acgcaggatt tgatacacaa gggcttcgtc gaagttgttg cgcagcgtgg
     4741 cgaaaatctg gatgccgggg tagggtggct ggctcccgtc gcgcgagacg aaatagaact
     4801 cgtactcgta gccggaaaga agatgatagc ccatcgcttc gagttcgtcc agcagcttgc
     4861 gtgcgacggc gcgcggcgcg gccaccgccg gacggccatc ttgaaaatac ggctcgcaca
     4921 agagacgtgc ggtcttgtcg gcccacggca gcacgcgata ggtgctcagg tcggggcgga
     4981 tacgcgagtc ggggaaaccg agttcttcga ggtagccggt gttcggcgcg acgccggcct
     5041 gcacgtcgaa gccgagttgg ccaagcagaa agttcagccc atgctcggtg aagtagcgca
     5101 ggtgccggac gggcacggtc ttcgagcggc tgatgccatg cgtgtcggtt tgttcaaagc
     5161 ggacatattc gaccccgtca gcggcgatct gtttttcgag cgcagccaag gggtcggtct
     5221 tacgtgcggg tgttctacgg gcaggcataa ggttctcctt ttacgtgtga ctgccgacag
     5281 atatgagtcc ctcacgctgt gagaggatga gttgtatcaa tccggcgaca agtgccgtgc
     5341 gcggcacaat gctgtcggcc tcgatgtact cgtccgggct gtgatcgagg ccgcctaccg
     5401 gcccgaggcc atcgagcacg gggacaccca gcgcggcgat gatattggcg tctgatgcgc
     5461 ctccagttgc cgcatcgttc acttcaaacc ccaaatcgcg cgcggcgttc cgcgcaaatt
     5521 cgacaagcaa ggcaaccgcc ggggtcttgg ccatcggcgg gtagtgaaag cctccggcga
     5581 cttccacctg tgttcgcgga atcgtcgtgt ggccggcaag atcagctatc gcctgggcga
     5641 gcgcctcggc cccggccggg tcggccgcgc gcgcatccac ccgcacccag gcctccgccg
     5701 cgacgacgtt ggacttcgtg ccgccgccga tcacgccggc gttcaccgtc actcctggcg
     5761 cgagaccgtt caaacggtgg agcgccatga tctggtgcgc gagctcgagg atcgcattcg
     5821 cacctttctc cggttcgacg cccgcgtggg cggctttccc tatcacccgg atcgtatatt
     5881 cacccgagcc cttgcgcgcg ctgacgatgt cgccattggc gcgcgcggat tccagcacga
     5941 gcaccgcatc catctcgcgc gcgatcggcg cgtacagcga ttgcgaaacg ggcgagctga
     6001 cctcctcctc gctgttgaag aaaaacgcca gctccgagaa gcctcgaaaa tccgtagtct
     6061 ggagcgcgcg cagcgcgtac atcccgacca gcaggccgcc cttcatgtcg cacgtgccgg
     6121 ggccgacgat cttcgcgccg gcgaggcgca tgggccgggc ggcggccgtg ccatcggggt
     6181 agatcgtatc gaggtggccc atcaacagca ggcgtcccac gccatcgccg cgcagtcgag
     6241 ccagccagca atcgccatat tcgacttgcg gcaagcgctg aacttcccag ccccaggctg
     6301 cgcaccgcgc gccgatccat tccccgaccg catccacccc ggccttgttg tgcgtgccgc
     6361 agtccacgtt gaccagcgcg gccaggtcgg agaggaagtt agggcgagcg gactcaagaa
     6421 aagcaatgat agggttcaca tcccaatttc caaatcccaa cttccaaatc ccaagggtta
     6481 ggcagggtcg agagaacgct tgcccttgtt gatgagggcg ctgaggatgc tcacaagttc
     6541 gacgctttct tgttccaatg cgtgaccttc ggaagactcc gcgccagcgg cgcagatcac
     6601 acggatccaa tagcgcgact cacgcgcttc ttttcgggag agtgtccact tgtacaccct
     6661 gtctttcggc gtgtcagcgc cgtcggcttc ttcgacattc gccccaatcg aagtggccgc
     6721 ttctctgcca tcccacctcc tcctttattt ttgggatttg gtatttggga tttgaactat
     6781 agcctcacag ttatcagagc atcgaactca gggaactgcc agttccccca gttccctgag
     6841 ttcctttaga tccgtccgcc tttacggcgg gggcaccttc tcgggcacga gttcggcgcc
     6901 gagcgtgaac ttgccgtcct tgacggcgat gacggtcacg cctttctgcg gcacgtgcgc
     6961 ctccgggctg aaggtgatcg agccggtgat gccagggaag ttctgggttt cctcgatggc
     7021 cttcttgatc gcagccgggt cggtcgaacc ggcccgcttg atggcatccg cgatcaggta
     7081 catcgtatcg tagccgaggg cggcgaaggc gttctccggg tcgtgtgcgt actcggcctt
     7141 gtaggccgcg atgaacttct tgatgccttc ggtgccgccg gtggcgtcga tcagggcgtg
     7201 ggtcgtgaag aacacattct cggctgccgg gcctgcgacg cttaccaggt ccggtgtgtc
     7261 gtagccgtcg ccgccgacga tcgggccggt gaggccggcg tcgcggaact gcttgacgac
     7321 cgggccgacg ttatagggca tcgccgagat gaagtagaag tcgggctgct ggggcagcgc
     7381 tttcaccttg gcgatctgcg ccgagaagtc ggtggcatcg gtttcgtacg agtcttcgag
     7441 gacgatcgtc ccccccagct cggtgaagcg tgctttgaaa tagccgctga gcaaggtggt
     7501 gtactcgaca cctttatccc acagcagata cgcggtctgg ccgtaattct tgaagccgta
     7561 ctccgcgccg acggctgcct gcacattatc gccgaagcag gccaggaaca tcatgtcgcc
     7621 gacctgtgtg ggaatcttgg gcgaggtggc gccggcggtg atgaacggca gcccggccgc
     7681 ctggatcgtc gggccggagg ccaacaccga gtcggtgtcg gtgaagccga tgacggcgac
     7741 cacttgatct tgctcgacga actgcttggc gatctgtgcc gtcacgtcca tcttgtactg
     7801 gctgtcgtgg acgaccaact ccacctggcg gccgagcaca ccgccggcgg cgttgatctc
     7861 tttcaaggcg agtttcgcgc cgttggccgc cggcacgtcg agtgcggact ccgcgccggt
     7921 gagggcgaac cccccgccga tcttgatcgg cccggtcgtc gccgctcccc cgccgcaggc
     7981 agccagcagc aggctggcga tgccggcgag agcgaaaagt ttgcctttca tggtggtctc
     8041 tcctccttaa gtgaatcgtg aatgtccggg caacccgcac agtgtaactc taactcccaa
     8101 ctctaactct aactcctcac tctaactcga tggctcacct cctttcataa acaggcgatt
     8161 gaaggcgatc tcgcgctggc cgagcaggcc cccgcgtcga aagatgatga ggaggataaa
     8221 gatcacggcc aggataatct ggctcatgcc atacagcagt gtggcgtctt cgatgcggcg
     8281 cagcgcttcg gccagcccga cgaccaacac cgtgccgatc gccgacccac tcaccgagcc
     8341 catccccccg acgaccagca tcgtgatcac gcggaaggtc aggtcgacat agaaaacggt
     8401 cggtgagaac gaggtgatga aatgtgcgta cagcgagccg gcaacggccg tgaaaaacgc
     8461 gctgacgacg aacgcgagca agcgcggcgg caggacagcg atgccgatag actgcgcagc
     8521 ccaccgatcc tcccgctgtg ccagcatggc cctcccaaag ggcgaccgct tgatccgcca
     8581 aaccacgtac agcgccacca gcgcccaggc ccacacccac cccaggttgg tgtagggcgt
     8641 gacgtttgag aacgtgcgcg agccgcgcgt gaagtgatcg gcgttcagca ggatgacgcg
     8701 gacgatgacg aggaagccca gcgtggcgac ggcgacgaag tgccccgaca gccgcatcag
     8761 caccagcccg acgagcagcg cgaccagcga gaccagcagg gcggtgacga gcgttgccgc
     8821 caggaagccg agtggaaacg ggccgaggtg aaaatcgaaa tggacgccgg caagccccgc
     8881 cggcaggttc ggcagatatt cctgcttctc gcgcagaggg agtgtcagga tcgcggatcc
     8941 atacgcgccc agcgccataa agccgatgtg ccccagcgag aacacgcccg tgaaaccgct
     9001 ggagagattc aggctgagga cgaggatcag aaagattccc aggttgatga acagcccctg
     9061 ccagtaggcg aggccggaag cttgcagaag cgccaggacg atgaccaggc tgaggagcgc
     9121 agtgatcggc ccggccgcgc ggcgcacgcg atggatgtcg atcattgcat ttcctcccgc
     9181 acgcgccggc gcaaaatccc gttaggtcga aacagcagca ccagaatcag cgcgacgaat
     9241 acgaacgcat cgcggtaact ggtgagagat gccggcagcc actgtttgag aaaggcctgc
     9301 gcctccggcg agaccaggcc cgccggcagg atgtcgccga tgccgggaat ggcggtcaga
     9361 aacacttcca gcccgcccag gacgtacccg ccgacgaccg cgcccgaaat gctgccaaag
     9421 ccgccaatga cggcagcgat aaaggctttc agcacgggag taaatccgat atatggattg
     9481 atctgcccgg cctgcaccgc gaacagcaga ccggcgacgg cggccaggcc cgagccaatc
     9541 gcaaaggcag tggcgatcac ccggttgatg ttaattccca tcaattgcgc agcggaaata
     9601 tcttcggcgg ctgcccgcat cgacaacccc aaggttgtgc gcgtcacgaa cactgagagc
     9661 aggacaagca agatcgcacc cgcgaccaga ctgacaatgt tcaagcgcgg cacagtaatc
     9721 ggccccaagg cactcactcc attcaagatc tcgggcgagg ggaatgcgat ttggatcggc
     9781 ttctgaatcc ggcgcgtgaa cagcaaggtg ccgttctgga gaatctggcc gacggcgaac
     9841 gaggtcagca aagccgccac gtcgggcgcg ccacggatgg ggcggtaggc cacgcgttcc
     9901 atgagcaggc ccaccatcac agtcccaccg atcatcagca acagcaccag gcccagcggc
     9961 aggttggcaa ataaggcaaa cgcggcgaag taagcggcca ccatcatcaa gtcgccgtgc
    10021 gcgaaattga tcaggcgcag aatgccgaac accatcgcca gcccgacggc catcagcgcg
    10081 tacaggctgc cgatgctcag cgcgttcacc aattgctggg tcacatacac gagcatgatc
    10141 acgttcccag gtacgtgtgc tcgacttgcg gattggcttt cagctcagcc gccgtttctg
    10201 ccaggataat ccgtcccgtc tccatgacgt agccccgatc ggcgattcca agcgcgagac
    10261 gcgcgttttg ctcgaccagc aggatcgtcg tcccggcgtg cttcaattgt tgaatcgtgt
    10321 cgaagatggc agcgaccagc aagggagcga ggcccagcga gggttcatcg agcagcagca
    10381 gcttcggccg gctcatcaag gcccggccga tggcgagcat ctgctgttcg ccgccagaca
    10441 gggtgccggc caactgccgg ctgcgctctt tgagcacagg gaaaagttcg tacactcgct
    10501 cgcggtcctg gcgtatgccg tccgggtccc gcctccgtac ggcgcccagc cgcagattct
    10561 ctgccaccgt gagacgaccg aagatgtgcc gcccctccgg gcagtgagag atgccgaggg
    10621 cgacgatctg atgcggcggg aggcgatcaa tcggctggcc gcgatactgc acgcgacccc
    10681 ccttcgggcg cagcaggccg gagatcgtct tgagcgtcgt cgatttgccg gcgccattcg
    10741 agccgatcag cgtgaccagt tcaccctccg cgacagtcag cgagacgccc ttgagcgcgc
    10801 ggatgctgcc ataatagact tcgacgtcat gcagttcgag cacgcaccca tgctccgatc
    10861 aggttcgaat ggtcttcccc taaataggcg gcgatcacct gtggatcggc ccgcaccgcc
    10921 tccggcttgc cctcggcaat gatccgtccg tagttcaaca cgatgatgcg ttcgcacagg
    10981 ttcatcacca gccgcatgtc gtgctcgacg agcacgatgg tcagtccgaa gcgcccgcgc
    11041 aggtcgagga tgagttggtg cagcgcgtcg ctctcggccg ggttcatgcc ggccgccggt
    11101 tcgtcgagca gcaatagcgt cgggccggta gcgagcgcgc gcgcaatctc caggcgtcgc
    11161 tggtaggcgt agggcaggct ggccgccggc cgctcggcaa agtggatgag gccgaaaagg
    11221 tcgaggtact ccagggcgcg cgctttcact ctcgcttcct ggcggcggaa gcctggcccg
    11281 ctaatcaacg tctcccacag ggatgtggcc gtccgcaact gcgccgcagc ctgaacgttc
    11341 tccaacaccg acagcgatcc aaacactcgg atgttctgga aggtgcgggc aatccccagc
    11401 cgggtgatgc gcgccggagg caggccggtg atatcctgat gctccaattg aatccggcca
    11461 gccgtcggaa cgagaaagcc cgtgagcaga ttgaagcagg tcgtcttgcc cgcgccgttt
    11521 ggaccgatga ggcccagaat ctcaccgggg cggacagcca ccgagaagtc ctgcagggcc
    11581 tgcacgccaa gaaaggactt ggccagtcca tgggcctgaa gaataggctc ggatgaatcg
    11641 gtcggcacga gtttcataga cgatggcagc ggtggaagcg ctgatcgtga gtggtgcgct
    11701 aaagtataat tccagtcagc gcgaagtcaa atttccgtga gcgcggcgtc gcttaaatat
    11761 aaccctcttc gcgccaacgt caagaccgtt cgaagcgcgc gctgctcacc ttcgtcgtcg
    11821 cgagctcgtg atcgcgcgac acatcgtgta taattaggac gtgaggtgag acatggctcg
    11881 gcaggcactc agagaagtga atgcgccata cacggtcaat tcagaggatg aagcgatcgg
    11941 gcgcgacact atcgttatcc ggcgcagcgg tgagccggta gcagtcgtgg tgccgtatgc
    12001 cgagtacgtc gaactgctgg cgcgcccatc cgcgcagcca tctgagcctg tatccggcga
    12061 tcccgacttt gaacgcgaac gcgctgcctt tcgtcgcctt ttgcctgaat tgctcaaagc
    12121 gcatcgcggc gaatgggtgg cgattgtgaa cgagcaggcg gttgagttcg gcccagactt
    12181 cggcagcgtg attcttcgcg tgcgcgagcg attcggccag cgagcggtgt acgttcagga
    12241 gattctcgaa aaacctcgtg tgtacaaatt cggatcccca cacgttatcc gctcatgatg
    12301 attccttacg atacttcgct catcccaccc gcaccctttc tgtccgctca tgtctcaaat
    12361 ctcacgggta gcgcagagtc tccaccgatc ccggccaagg tcgataccgg ggcagatgtg
    12421 acggctatcc ctgctaactt ggtgaaccag cttaacctcg tgctggcaag cgaaatccaa
    12481 gtggaagggt ataacgggca tcgtgccaca ctcccctgct atgacatcgt cttgcgcgtc
    12541 gcacatttgc gtgtggtcgg tctgtcggtg atcagtttct cggaagacta tatactgctc
    12601 ggccgtgacg tgcttaatct ccttcgcttg cttcttgacg gcccggccct gtggcttgaa
    12661 gttctgtccc ctcaatctcc ttcgtagcga acgttgacgc caaccggcac tgaagcagcg
    12721 cgagtacatg gtaaggcccg caacagcgat ggcgagaagg cggcctcacg aaatacactg
    12781 gcaattctgt cgcctatgga agccgattgc gcaaacaggt attcgggcga taatagagga
    12841 ggctaattgc gcgaatttgg ctttagtgtg gagaaataaa atgcaaggct ggcgcaacct
    12901 caaccacggc gacgatcgca ttgaaatcta cgacacattg gccgaggccc gcgagagatt
    12961 tggtgagaat tttcaaggct tcgtgttcac cctgacccgc gctcaagccg aagccttgtt
    13021 gcagggcaag gtggtggcgt tcgacattgg cggcagagag tatgccggat ttatgctggt
    13081 gcaggaaccc cccggcccat gagcagtctc ccgcgcgatt ttcgcccatc cttatccagg
    13141 tagtcagcca agcagcgttg tgcggtttcg atgtcttgct ttggcgtgat gatagcacta
    13201 ttgttattgc ctgtcgagac aaccgcttgc gatacggcac gtgcagcagg aaaaggcccg
    13261 cgcggggagt tttgaggatg ttcgcggtat ccggagtgca agggtacgcc aaacgtggag
    13321 tcatttcgtg ctggttagct atcaccgcta ctgcgcctgg ttcaggaaat ccagcaggcc
    13381 ggcgatggct ttcaatacag gctggcccag acccgtcaga cccagcagtg gcactatttg
    13441 cgaggtcgta aatttggcta gaatcctggt attgaatggc cagacagaga tgtgctgatt
    13501 ctctcgataa atctgctgca tccgttccag tttcctggcc atcttctcgc tttccaccgg
    13561 atccaactcg tccgcccggt ccagcagttc ccgcgccagg tgattgatgc tttggcccag
    13621 ttgatccagc tgccgctgaa tcaaagcccg tttggcgacc atgatctgat gcacactcca
    13681 cagcggcaag aagaaactga tggcggcaac ggcgagcggt accaggagga gtgtggagaa
    13741 caagggtgta taccatgccg ccagatcgct gtattgttcc ggcaggatcg gccccagaat
    13801 gatccagccc cctagatgca ctcccgctgt gctgacgatc agcgcattcc acaggcacag
    13861 attgccgatt ggcgaaagtc ctccgcaacg atccggatgc cctagctgga gtgccagatc
    13921 gaacttcatg cccatctgcc agatctggta gccggcgacg atcatccgcc aggccaacaa
    13981 gccgatgatg tagccgatga tgaactcgat gatgaactgt ccgatgagca ccggatcggc
    14041 gaacgggtct aggagagcct tccatccacc cagccggtaa gaatccacga cgatccagcc
    14101 ggctcccaag agcccgatta tcacacccat gatccgctgg cgggagtcat tcaaccatcg
    14161 cttcacattg tcgataaacg ctcggtattg ctcctcggga ggcgtggact cctcggtagt
    14221 gaccgaggcg ctgtgggctg cgtcggttgg ccgcttgggc ctggcgcgaa cagtgtttcg
    14281 atcccagagg atgcgaaagg tctccgggat acgctgcatc agaacttgaa aggcgaaaag
    14341 gctcaaggcg gcagccacga tcccggagaa gtacatgacg ttccccggcc cgacctcgaa
    14401 agctgtgagg cggacggtgg tcaccagcag catcaaccag ggcaggaagc gtagtgccca
    14461 gcgcaaggca cggctctgga ctccctgaaa gctctctaac gcttcagaga cgctgccgga
    14521 cgttggcgga ggactatgtg gcaagcgcgg agacgtgact gcttctgccg gcatgggcgc
    14581 tcatctcctt cattgtgagc gtttctcgca acttgatatc gcgagttgcc cgatctcaaa
    14641 cacagtctac acccacgtgc gtaaaaagca aatcgcctag gatcgtctgg gatgcggttc
    14701 tggggcagac ctaacccccg gtcccactcc cttccccggc acggggaggg gccggggaaa
    14761 gggtcgccac gcggcgatca aattcgcgtt caaggtgtgt tatctaaaat tctcagccag
    14821 atgagggtct cgacctcaga caacagagtt tcggcgttta cgcatacaca ctttgattcg
    14881 gcccggcgta cgagtcatac acgtgctgat acaccgcctc gcatttctgc tgatacaggt
    14941 cgtctgtata ggcccgaggc agatcttcca gcgcccgctt gatcgccacc cgcacggcgg
    15001 cgcgagtctg ctgccggctg cgccattcga gcaccagccg ctcggccttc agcgtgtcga
    15061 gcaggtcgcg cgcgatttgc ttgatcttct cccgctcgcg tttgatgagc tttgggctgg
    15121 gacgggtgag gatgtcgaag atggcgagtt cttcttcgga taggttctcg gcgatgccgc
    15181 gcttctcttc ctcgtcgagt tggcgcgcga aactgaccaa ctgtgcgaac acagcgtcga
    15241 catccgtcgc gccggcgttg tagtcctcga tcatgcgctg gaa
//
LOCUS       DBNHXB010000442         4279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4333750, whole genome shotgun sequence.
ACCESSION   DBNHXB010000442 DBNHXB010000000
VERSION     DBNHXB010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4333750"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1249
                     /locus_tag="AC162G_17440"
     CDS             281..1249
                     /locus_tag="AC162G_17440"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR5077642.1"
                     /translation="MELVLIVGALIGLSVLIFFIGVARALEAPTSDRLEDYLGDQTAR
                     LRAEQSRKSSGLGASAGELVQGFDKVLRSVSAGDRLAQALRRAGLRLTVTEFALIWLL
                     SIVGALLAGYFLTRSALPAVMVGFLGALLPYIFVKFRQGSRLRAFNNQLGTVLMQLSG
                     SLRAGYGMSQAVDFVAREMPAPAGPEFGHVVREVKLGLSLMDALDNLAERVGSDDLML
                     IVTAIRIHHETGGNLAEILETVGETIRERVRIKGELRSLTAQQRYSGYALAVLPVAVF
                     ACLMLLNPAYESRLFAPGITLCIPIGALIMMLFGFLAIRKIVSIEV"
     gene            1341..2291
                     /locus_tag="AC162G_17445"
     CDS             1341..2291
                     /locus_tag="AC162G_17445"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR5077643.1"
                     /translation="MPYEILVGLAVALSVMLFWMGRRFATSPLEVDDQLERYLIDDPS
                     VDSRYTIATNPSRLTFTERVLIPLWRSALTRLGAMAPQRNADAIGRRLETAGRPYGLT
                     VLNFLGLKFIGATIVGVVGLLFGAVVLHQSLLVSVLFVVAFGVLGFYMPNIWLSGVIR
                     RRQREIMRALPDALDMIVVCTEAGQGLDQAFKRVSARWRNALTEEFMRILTEITLGRT
                     RREALTGASDRIQLPDVSNMITAVIQAEQLGVSIGSVLRIQANQMRIVRRQRAEELAR
                     EASIKLLFPLVFLIFPAMFAVLLGPAIPLLIQTFSGTLSP"
     gene            2402..4042
                     /locus_tag="AC162G_17450"
     CDS             2402..4042
                     /locus_tag="AC162G_17450"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5077644.1"
                     /translation="MEATLHMENDPLYEQGLKYFGQGKWTEAIACFTQLQASYPDDAR
                     VSQFLETARLRAAMGTGLQRGARTEARSRWLRVLSRLGILLVLAAIAGAVYLAYQTYA
                     VPAQVAAARLERLEGLRQTAQTQMASGQYAQAILTFQAILEEEPEDALASAGLEQARK
                     LEQAGALYAQATTALGAGDDAEAMRLLQEINAIDPNYRDVATLMAGIKARESLTEAYE
                     QALKTYESNDWEGSAQAFEAIRTVDSSFRSSEIGGYLFTAYLELGNRQIAEADTIAAV
                     EVAAGYFEKALSVRPLDERADTARRTAETFLAGAEAYQAKDWGTVIGKLSIVYETQPT
                     YYGGKVAEWLYEAYLTTGDDFMGKGDPFSARDRYAEAIRVAPGDAQRAEAQTRYNRAN
                     RLTTPTPTPRPSPTALPSGYVAPAYTRRATGTPDPYPFALIGTTYLPNTINGEGCKWA
                     GVAGRIYDRSGAPLTADTLGVRVTGPVDVGVAAGSNTIIGESGWIVQFDVRAKVIEGY
                     LQVYYKDQPASQLIPFATRASCYENMLIIDIQQMKALP"
BASE COUNT          728 a         1365 c         1437 g          749 t
ORIGIN      
        1 cgggttccca agcaggcgct gggtcagtga agcacagaca cggattcggt cacggattgc
       61 acggatgcag cccaaagttg ggacggcagt tgcactgccg ttcccaatgg gcggttttcc
      121 aggcaggcgc tgagtcagta aagggcgaat acggatttgg tcacccccgc agacccccgc
      181 acacagcagg cgagggcagg cggattgcac ggatgaggtg ggcgagtccc actgatcact
      241 gatcactggt cactgatcac tggtcggcaa aaggagcaac atggaactgg tgttgattgt
      301 cggagccttg atcgggctgt ctgtcttgat tttcttcatc ggcgtggcgc gcgcgctcga
      361 ggcgccgacg tcggatcgcc tggaagacta cctcggcgat cagaccgcgc gcctgcgcgc
      421 cgagcagtcc cgcaagtcga gcggcctggg cgcgtcggcg ggtgagctgg tgcagggctt
      481 cgacaaggtg ctgcgctcgg tgtcggcggg cgaccggctg gcacaggcgc tgcgccgcgc
      541 cggcttgcgc ctgaccgtga cggagttcgc gctcatctgg ctgctgagca tcgtcggcgc
      601 cttgctcgcc ggttacttcc tgacgcgcag cgcgctgccg gcggtcatgg tcggcttcct
      661 gggcgccctg ctgccttaca tcttcgtgaa gttccgccag ggcagccgcc tgcgcgcttt
      721 caacaaccag ctcggcaccg tgctgatgca gttgtccggg tcgctgcgcg ccggttacgg
      781 catgtcgcag gcggtcgact tcgtggcgcg cgaaatgccg gcgccggccg ggccggaatt
      841 cgggcatgtg gtgcgcgagg tcaagctcgg cctgagcctg atggatgcgt tggacaacct
      901 ggccgagcgc gtcgggagcg acgacctgat gctcatcgtc actgccatcc gcattcacca
      961 cgagacgggc ggcaacctgg ccgagattct cgagacggtg ggcgagacga tccgcgagcg
     1021 cgtgcgcatc aaaggcgagc tgcgctcgct cacggctcag cagcgctatt ccgggtacgc
     1081 gctggcggtg ctcccggtcg ccgtgttcgc gtgcctgatg ttactgaacc ctgcgtacga
     1141 gtcgcggctg ttcgcgccgg gcatcacgct gtgcattccg atcggcgcgc tgatcatgat
     1201 gctgttcggt ttcctggcga tccgcaagat tgtgtcgatc gaggtctgaa cgacagctcg
     1261 ttcgggtggc agttgcactg ccgcccgccg cagtgcaact gcggcgggaa ccacgtggtg
     1321 gcgacgagga gtgtcatctt atgccatacg aaatcctggt aggtctggcg gtcgcattga
     1381 gcgtcatgct cttttggatg gggcgtcgct tcgccacgtc gccgctcgaa gtcgacgatc
     1441 aactggagcg ctatctcatc gacgatccga gcgtggattc gcgctacacg atcgcgacca
     1501 atccgagccg gctcactttc accgagcgcg tgctcatccc gctgtggcgg agtgcgctga
     1561 cccgcctggg cgcgatggcg ccgcagcgca acgccgacgc gatcggccgg cggctcgaaa
     1621 cggccgggcg cccttatggc ctgaccgtcc tgaacttcct cgggctgaaa ttcatcggcg
     1681 cgacgatcgt cggcgtcgtc ggcctgctgt tcggcgctgt cgtgctgcat cagtcgctgc
     1741 tggtgagcgt cctgttcgtg gtggcgtttg gcgtgcttgg gttctacatg ccgaacatct
     1801 ggctgagcgg ggtgatccgg cggcggcagc gggagatcat gcgcgcgctg cccgacgcgc
     1861 tcgacatgat cgtcgtgtgc accgaagcag gccagggcct cgaccaggcg ttcaagcgcg
     1921 tcagcgcgcg ctggcgcaac gccttgaccg aggagttcat gcgcatcctg accgagatca
     1981 cgctgggccg cacgcggcgc gaagcgctca ccggggcttc cgatcgcatc cagttgcccg
     2041 acgtgtcgaa catgatcacc gccgtgatcc aggccgagca gttgggcgtg agcatcggca
     2101 gcgtgctgcg catccaggcg aatcagatgc gcatcgtgcg ccggcagcgc gccgaagagc
     2161 tggcgcgcga ggcgtcgatc aagctgctgt tccccctggt ctttttaatc ttcccggcga
     2221 tgttcgccgt attgctgggc ccggcgattc ctttgctcat tcaaacattc tcaggaacgc
     2281 tctcgccata ggccggtaaa tacacgcagc gctccggccg ccgcccgcgg cggcccagcc
     2341 cgcgccgagg acggcgccgg gaacgagatg taaatagtca tcaggcggta gggagtcaac
     2401 aatggaagcg acacttcaca tggaaaacga tccgctctac gagcagggcc tcaagtactt
     2461 cggccagggc aagtggacgg aagccatcgc gtgcttcacg caactgcagg ccagctatcc
     2521 cgacgatgcg cgcgtgtcac agtttctcga gacggccagg ctgcgcgccg cgatgggcac
     2581 cggcttgcag cgcggcgcgc gcaccgaggc gcgctcgagg tggctgcgcg tcctgtcacg
     2641 gctcggcatc ctgctcgtgc tcgccgccat cgccggcgcc gtctacctgg cgtaccagac
     2701 gtacgccgtc ccggcgcagg tcgcggccgc ccgcctggag cgcctcgagg gactgcgcca
     2761 gacggcgcag actcagatgg cctccggcca gtacgcccag gcgatcctga ccttccaggc
     2821 gatactcgag gaagagccgg aggatgcgct ggccagcgcc ggcctggagc aggcgcggaa
     2881 actggagcag gccggcgcgc tgtacgccca ggcgacaacc gcgctgggag cgggcgacga
     2941 tgccgaagcg atgcggcttc tgcaggagat caacgccatc gatccgaact accgcgatgt
     3001 cgccaccctc atggccggga tcaaggctag ggaatctctg acggaagcct acgagcaggc
     3061 gctgaagaca tatgagtcga acgactggga aggctcggcg caggcgttcg aggcgattcg
     3121 cacggtggat tccagtttcc ggtcgagcga gatcggcggg tatttgttca cggcttacct
     3181 ggagctgggc aatcgccaga tcgcggaggc ggatacgatc gccgccgtcg aggtggccgc
     3241 cggctatttc gagaaggcgc tctcagtgcg cccactcgac gagcgggccg acaccgcacg
     3301 ccgcacggcg gagactttcc tcgccggcgc cgaagcctac caggcgaagg actggggcac
     3361 ggtcatcggg aagctaagta tcgtctacga aacgcagccc acctactacg gcggcaaagt
     3421 ggccgagtgg ctttacgaag cgtacctcac gacgggcgac gatttcatgg gcaaaggcga
     3481 tccgttctcg gcgcgcgatc ggtacgccga ggcgatccgc gtcgcgccgg gcgacgctca
     3541 gcgcgctgag gcgcagacgc ggtacaacag ggcgaaccgc ctgacgacgc cgacgccgac
     3601 gccgcgcccc agcccgacgg cgctgccgtc cggctatgtc gccccggcgt acacgcgtcg
     3661 cgcgaccggc acgcccgatc cctacccctt cgcgctcatc ggcacgacct acctgccgaa
     3721 cacgatcaac ggcgaaggct gcaagtgggc cggcgtggcg ggccgcatct acgatcgcag
     3781 cggtgcgccc ttgacggcgg acacgctcgg cgtgcgggtg accgggccgg tcgacgtcgg
     3841 cgtggcggca ggttctaaca cgatcatcgg tgagtcgggc tggatcgtcc agttcgacgt
     3901 gcgcgccaag gtgatcgagg gataccttca ggtgtattac aaagatcagc cggcgtcgca
     3961 actgatccca ttcgcgacgc gcgcttcgtg ctacgagaat atgctgatca tcgacattca
     4021 gcagatgaag gcgctgccgt agacgagcat ccaacttcca acctccaacc tccaatagga
     4081 gcacacgggc ctcgtcgaga gatgaggctc gtgtcttttt gtcgccgctt gctgtataat
     4141 ggttgcggtc caccggccgt gtcatgccga gaatcccaca tcgccaggtg gcgatatgcc
     4201 tgaaatcaga aacttacgca cggcacgtgt catgctgagc atcttttcgc gaagcatctc
     4261 tgccacggct ggcaacgtt
//
LOCUS       DBNHXB010000443         6972 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_307147, whole genome shotgun sequence.
ACCESSION   DBNHXB010000443 DBNHXB010000000
VERSION     DBNHXB010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_307147"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="AC162G_17455"
     CDS             <1..197
                     /locus_tag="AC162G_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077645.1"
                     /translation="ARRAMCCTVRAGTPSSFFKIRPQRLTADLVGQDELGARDAAAKC
                     MTFVILFLSHPILLQLNQSG"
     gene            310..723
                     /locus_tag="AC162G_17460"
     CDS             310..723
                     /locus_tag="AC162G_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077646.1"
                     /translation="MPESIANGLRVLGRRLLRILLFYKILLANLALVALAGVAAALVT
                     AWHIGRFPDILDIDLIMLVVVTALVISCVVNYVVLKLALVPLDRIRAAIDEFQQGKLG
                     IRIDPGPFGVRVEDTVLVGADGPTVLTTYPRQLEK"
     gene            845..1267
                     /locus_tag="AC162G_17465"
     CDS             845..1267
                     /locus_tag="AC162G_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5077647.1"
                     /translation="MAREERRWFGFLIVVAVLFNAITLSPLVPWQSWSLWSQPTPDRR
                     VTIEMANYEFGLPPQGIEVKANEYVEFVATSRDVTYGFGVFRQDGTLVFQMQVLPGRE
                     NKIVWKFDTPGTYDVRSTEYSGPRHPEMFIEDAIRVTP"
     gene            1281..2918
                     /locus_tag="AC162G_17470"
     CDS             1281..2918
                     /locus_tag="AC162G_17470"
                     /inference="COORDINATES: protein motif:HMM:NF012343.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR5077648.1"
                     /translation="MTRIGWLEKWTLRFAVVGLLFLALAILEGALMRVQLVDREALRG
                     MESLLMNVRLVGGEVSSTDVFYSMLTAHPIVGIYGFAYMCVMGAFYFLVPFLLKKEIR
                     HKRLVPINFWLHIAGVLIAWGAGFFVLFNGLYTLYWPLPVSYDRVPLIGSIIFGLGLV
                     LIEVNVLLFVFNLFSTVLTRSEPNAPGFWRTLFSAIGIPRWLRRGGKTSDDARPHPDE
                     LPVFIVAVGRGSIDTAINAIVMLSAGALLLVYGLPTLLAGVRLDSAAIDSLLYKNWFW
                     WGLDMIADGNVLMYTAGTWYLLIPLLVNRKLYGESVVRWVILADLLVSLFVWSHHLLA
                     DRPQPFGLQLLSGQFVTWGEFLTMGLTIFTVMMTIWLARPVKFSPALKFVMGSIFGFI
                     VGGMAGLTQANVGLNLVLHNTQWVIGIHAHMMLLGGLAMLLFAVIYALVPLLTNLQIR
                     SQRLVNYHFWGWMVGAVLMAYAMGMAGSQGMLRRTLYTTAQYQPYMVVALIGGLLMSA
                     AFVLFLVNLIGTLGWSNVLSLVLPERWLRSQPAQAAT"
     gene            3025..3573
                     /locus_tag="AC162G_17475"
     CDS             3025..3573
                     /locus_tag="AC162G_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HIR5077649.1"
                     /translation="MPPHCDSMDGPVVIACRKALEAENVDLILPYVPKQGEEEVVRAF
                     KKVLSVRKQPNGAREIADLYFFETVVRVHRAGEGAPYTGLKRAGLSEGPVIPVAEKAI
                     ETGSPDDLLKVLTDIVRAEAQKRFDRAMRLRKHADGPVDEAREYVEAMLGLQVWAHKL
                     YTCARSEPHAEVREHEEVHNHG"
     gene            3696..3986
                     /locus_tag="AC162G_17480"
     CDS             3696..3986
                     /locus_tag="AC162G_17480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR5077650.1"
                     /translation="MFVNIIHFPPIKAGKDAEFREWFAWSNEEYAKHKGFIHRWLLRS
                     REGGNYVGLVEHESYETFMAMHTSSTQAEAGERVKPMFDGDPTPQFYEVVLG"
     gene            4105..5049
                     /locus_tag="AC162G_17485"
     CDS             4105..5049
                     /locus_tag="AC162G_17485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR5077651.1"
                     /translation="MKESEDIRGLKLALGVYILILALKLVVYFMTGVMALLAEALHTL
                     SDIFISGFLLVAAIWSRKKADETHMFGYGRAQNVAALVAATLFISFTSYKLYEEAIPR
                     LFQSGEATYQNLELALGVIIVSMLLAAAPLIKLWTQKERGPAAKAQLTESINDELGLL
                     AALLGTLFIIWGQPIADPIATIIVATIIAINAIGLFRENSSFLLGRSPGPEYLARLEG
                     LARSVPGVVGVHDVRAETIGPGVVHAGLHIEVPRGLPIEEADRIAREVEERVHAGTDS
                     GYCVIHMDPADSEAARQPMPAEKSPTEMLEEEHLPMKQ"
     gene            5061..5510
                     /locus_tag="AC162G_17490"
     CDS             5061..5510
                     /locus_tag="AC162G_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR5077652.1"
                     /translation="MTIAIPQPLQVEHEELHNELKQAIAAGGKVGEAATAVAMALHPH
                     FGSEEEYALPPLGLLPALAEGIVTPEMADVLPLTDKLKAELPHMLEEHQAIVAALKKL
                     QEAAQEENKPEYAHFAEKLMLHARMEEEVSYPTTILIGEYLRLRLGK"
     gene            5530..5895
                     /locus_tag="AC162G_17495"
     CDS             5530..5895
                     /locus_tag="AC162G_17495"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5077653.1"
                     /translation="MKTMHLLEGLEFHDKNPNAEPLHVDKNGRVLRFMLKPGQSITEH
                     SAPSSPFYVVVLRGQGMFAGGDGQEQKLGPNTLLIFDPGENHVIRALDEELVFVGFLQ
                     GVPGMRPGKVGGKMARQRR"
     gene            5958..6188
                     /locus_tag="AC162G_17500"
     CDS             5958..6188
                     /locus_tag="AC162G_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077654.1"
                     /translation="MKAHDLVIAILAVLVFLLISLPYQFAYSYWAESTALWVVYIVVG
                     VILAVFVFFNFLQDWRRLVAEAEGEEKKEVAP"
     gene            6185..>6972
                     /locus_tag="AC162G_17505"
     CDS             6185..>6972
                     /locus_tag="AC162G_17505"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5077655.1"
                     /translation="MIDAQESKRRKVGLGLLSIVVVVFIALSGLSFVQERGRPDPEMI
                     LFQGYSAVDGKRVFQAYNCMGCHTMLGNGAYFGPDLTKIYEDGGAAWLKAFLSAPGTW
                     PTKAVVDLRVEQLRQSGDVDVASTDAYYQQYPHVLEAVELFGGKRTVMPNLVLKPDEI
                     AALTAFFNYAAEIDTAGWPPARQADAAVVERLQAEFGVVPPTAVETAPTPASPVSLEV
                     QGKQLAGELGCLACHSTDGIQRSGSTFKGLFGATVPLQDGSTVTV"
BASE COUNT         1334 a         1998 c         2209 g         1431 t
ORIGIN      
        1 gagcgcgacg ggcgatgtgc tgcaccgtcc gcgctggtac acccagttcg tttttcaaga
       61 tacggccgca acgcctcacg gccgacctgg tcggtcagga cgaactcggt gcgcgtgacg
      121 cggcggcgaa atgtatgaca ttcgttatcc tttttctgtc acatccaata ctgcttcagc
      181 tcaaccagtc cgggtagcct tggatagggg ccacccgtgg aaagtcgtag cgtccaaccg
      241 aatatccaga atgaacagtg caccggccag ggtcgattca ctccttctgt tcggcgcatt
      301 ttgtttcgga tgccagagag catagcgaac ggactccgcg tccttggccg gcggctcctg
      361 cgcatccttc tcttttacaa aatccttctg gccaacctcg cgctggtcgc cctcgcgggc
      421 gtggcggcgg cgcttgtcac ggcctggcac atcgggcgat tccccgacat cctcgatatc
      481 gatttgatca tgctggtggt ggtgacggct ctcgtcatca gttgtgtcgt caactacgtg
      541 gtgctcaagc tcgcgctggt tcctctcgat cgcatccggg ccgccatcga cgagttccaa
      601 cagggcaaat tgggcatacg gatcgatcct ggccccttcg gcgtgcgtgt cgaggacacg
      661 gtgttggtcg gcgccgacgg gccgacagtg ctcactacct atccgcgcca gttggagaag
      721 tgaggaggta acatatcatg tacatcaccg acgtaatctg gtggtcgtat acgctgttca
      781 ttgtcgcaac cgcgctgttc atgatctggt tcaccctgaa ggtgaggcag agaggagatt
      841 agccatggcg agggaagagc gacgctggtt cggtttcctg atcgtggtgg ccgtgctgtt
      901 caacgccatc acactttcgc cgctcgtgcc ctggcagtcc tggtcgctgt ggtcccagcc
      961 gacgcccgat cggcgcgtga cgatcgagat ggcgaactac gagttcggac tgccgcctca
     1021 aggtatcgag gtcaaggcga acgagtacgt tgagtttgtg gcgacttcca gagacgtgac
     1081 ctacgggttc ggcgtgtttc ggcaggacgg cacgctagtc tttcagatgc aggtgctgcc
     1141 tggtcgtgag aacaagatcg tgtggaagtt cgacacgccg ggcacgtacg atgtccgttc
     1201 caccgaatac tcaggccccc gacaccccga gatgtttatc gaggatgcca tccgtgtgac
     1261 cccatgaagg aggcgcagcg atgactcgca tcggttggct ggagaaatgg acgcttcgat
     1321 tcgctgtggt gggcctgctg ttcctggcgc tggcgatcct ggaaggcgcg ctgatgcgcg
     1381 tgcaactggt ggaccgggag gcgctacgcg gcatggagtc gctgctgatg aacgtccggc
     1441 tggtcggcgg cgaggtctcg tcgaccgacg tgttctattc catgctgacg gcccacccaa
     1501 tcgtgggcat ctatggtttt gcctacatgt gcgtgatggg cgccttttat ttcctggtgc
     1561 ctttcctgct gaagaaagaa atccggcata agcggttggt tccgatcaac ttctggctgc
     1621 acatcgccgg tgtgctgatc gcctggggcg cgggcttctt cgtcttgttc aatggcctgt
     1681 acacgctcta ctggccgctg ccggtgtcgt acgaccgcgt gccgctaatc ggaagcatta
     1741 tcttcggctt gggcctggtt ttgatcgaag taaatgtgtt gctgtttgtc ttcaacctgt
     1801 tcagcaccgt cctcacccgc agcgagccga atgcccccgg cttctggcga acgctgtttt
     1861 cggcgatcgg cataccgcgc tggctcagac gaggcggaaa gacttccgac gatgctcggc
     1921 cccaccccga cgaactgccg gtgttcatcg tcgccgtggg tcgcggctcc atcgacaccg
     1981 cgatcaacgc catcgtcatg ctgtcggctg gcgcgctgct tctggtgtac ggcctgccga
     2041 cgctgctcgc gggcgtgcgc ctggactcgg ctgcgatcga ctcgctcctg tataagaact
     2101 ggttctggtg ggggctggac atgatcgccg acggcaacgt gctcatgtac accgccggca
     2161 cgtggtatct cctcatcccc ttgctcgtga accggaagct gtacggcgag tccgtggtgc
     2221 ggtgggtgat cctggccgac ctgctggtgt cgctgttcgt gtggagccat cacctgctgg
     2281 ccgatcgacc tcagccgttc ggcttgcaac tgctctcggg ccagttcgtc acctggggtg
     2341 agttcctgac gatgggcctg acgatcttca ccgtgatgat gaccatctgg ctggcccggc
     2401 cggtgaagtt cagcccggcg ctgaagttcg tcatgggttc gatcttcggg ttcatcgtgg
     2461 gcggcatggc tgggttgacg caggcgaatg tcgggctaaa cctggtgctc cacaatacgc
     2521 agtgggtcat cggcatccac gcccacatga tgctcctggg tggcctggcg atgctgttgt
     2581 tcgccgtgat ctatgcgctg gtgccgctgt tgaccaatct gcaaatccgc agccagcgat
     2641 tggtgaacta tcacttctgg ggttggatgg tgggcgcggt gctgatggcc tacgccatgg
     2701 gcatggccgg ctcgcagggc atgctgcgcc gaacgctgta cacgacggct cagtaccaac
     2761 cgtacatggt agtcgcgctg atcggcgggc tgctgatgag cgcggcgttt gtcctgttcc
     2821 tggtcaacct catcggcact ctcggatggt ccaacgtgct cagcctggtc ctgcccgagc
     2881 ggtggctgag atctcagccg gcgcaagcgg cgacgtgaga ctctgacgaa tcgaagcgtg
     2941 ataaggaggt tcgatcatac ttgaatgctt tcgcttctct gatcatcgtg tcaaacactg
     3001 catgaaatga aaggagattt ggtcatgcca ccccattgcg actcaatgga cgggccggtt
     3061 gtcatagcct gcaggaaagc gctggaggct gagaacgtcg atctcatcct tccgtacgtg
     3121 cccaagcaag gtgaggagga agtcgtcagg gcgtttaaga aggtcttgtc cgtgcggaag
     3181 cagcccaacg gggccagaga aatcgccgac ctgtatttct tcgaaacggt cgtgcgcgtg
     3241 catcgagcgg gcgaaggcgc tccgtacacc ggcctgaagc gggccgggct gagcgaaggg
     3301 ccggtgattc cggtggccga gaaggcgatc gagaccggct cacccgacga tctgctgaag
     3361 gtgttgacgg acatcgtgag ggccgaagcc cagaagcgat tcgatcgcgc gatgcgcctg
     3421 aggaaacacg ccgatgggcc tgtagacgag gcgcgcgaat acgtcgaggc catgttgggc
     3481 ctgcaggtgt gggcgcacaa gctctacacg tgcgcgcggt ccgagcctca cgccgaagtc
     3541 cgtgagcacg aagaagtcca caatcacgga tagacacgac gaaagtctca cagtggtata
     3601 tcgtccggca accgggccac ccaatcgaac tgaatctacc ggcaggtgac gtaatccccc
     3661 agccggctga atagactcat cagaaggagt ctgcgatgtt tgtgaacatt atccacttcc
     3721 cgccgatcaa ggccgggaaa gacgccgaat ttcgcgagtg gttcgcctgg tcgaacgagg
     3781 agtacgccaa gcacaagggc tttatccacc gctggctttt gaggtcgcgc gaaggtggca
     3841 actacgtcgg tcttgtcgag catgaaagct acgagacctt catggcgatg cataccagct
     3901 ccacgcaggc cgaggccggc gaacgggtga aaccaatgtt tgatggcgat cccactcccc
     3961 agttttacga ggttgtgctg gggtaaacct cgccgatcat ggcaaacgat cgggcgcatc
     4021 gatggtccag ggcctctgct cggcaatctc gacctgggcc atcgctcgcc ggttggacgc
     4081 ttccaggcat acggagggcg catcatgaag gaatcagaag acatacgcgg cctgaaactc
     4141 gcgctgggcg tgtatatcct tatccttgcc ttgaaactgg tggtgtactt catgaccggt
     4201 gtcatggcgc tgctggccga agcgctgcac accctgagcg acatcttcat ctccggcttt
     4261 ctgttggtag cggctatctg gtcgcgcaag aaagcagacg agactcacat gtttggctac
     4321 ggccgggcgc agaacgtggc cgcgttggtg gccgccactc tgttcatttc gttcaccagc
     4381 tacaaactgt acgaggaagc gatcccccgg ctctttcagt ctggggaagc aacctatcag
     4441 aacctggagc tggcgctggg ggtgatcatc gtctcgatgc tcctcgctgc ggctccgctg
     4501 atcaagttgt ggacacagaa ggagcggggg cccgcggcca aagctcaact cacggagtcg
     4561 atcaacgacg aattgggtct gctggctgcc ttgctgggaa cgttattcat catctggggg
     4621 cagcccatcg ccgacccaat cgcgacgatc atcgtggcca cgatcatcgc catcaacgcc
     4681 atcggcctgt ttcgagagaa ttcctccttc ttgctggggc gttcgccggg gccggagtat
     4741 ctggcccgac tcgagggcct ggcccgctct gtgccaggcg tggtgggggt gcatgacgtg
     4801 cgcgcggaga ccatcgggcc gggcgtcgtt cacgccgggc tgcacatcga ggtgccgcgc
     4861 gggctaccca tcgaggaagc cgatcgcatc gctcgcgaag tggaggagcg ggtgcatgcc
     4921 ggcaccgact ccggctactg cgtcatccac atggatcccg cggattcgga agccgctcgg
     4981 cagcccatgc cggcggagaa aagcccgacg gagatgctgg aggaagagca tctccccatg
     5041 aaacagtagg gagtgtgcca atgacaatcg cgatccctca gccgttgcaa gtcgagcacg
     5101 aagagctgca caacgagctc aagcaagcca tcgccgcagg tggcaaagtg ggagaagctg
     5161 ccacggcggt tgcaatggca ttgcacccgc attttgggag tgaggaagaa tacgcgctgc
     5221 caccattggg gttattgccg gcgctggcag aagggatcgt tacgccagag atggctgacg
     5281 tccttccgct gaccgacaaa ctgaaggcgg aactcccgca catgctcgaa gagcatcaag
     5341 cgatcgtcgc cgcgctcaag aagctccaag aggcagcaca ggaagaaaac aagccggagt
     5401 atgcccactt tgccgaaaaa ctgatgctgc acgcgcggat ggaggaggaa gtctcgtatc
     5461 cgaccacgat cttgatcggc gagtatctcc gactgaggct cggcaagtaa gacccaggag
     5521 gtttatacga tgaagaccat gcatttgctg gaaggtctgg agttccatga caagaacccg
     5581 aacgcagagc cactgcacgt ggacaagaac ggccgagtgc tgcgcttcat gctgaagccc
     5641 gggcagagca tcacggagca cagcgcgccg agctcgccct tctacgtcgt cgtgctgaga
     5701 ggacagggga tgttcgcggg cggcgacggc caggaacaga aactcggccc caacacgctg
     5761 ctgatctttg accccgggga gaaccacgtg atccgggcgc tagacgagga gctggtcttc
     5821 gtgggcttct tgcaaggagt gccgggcatg cgaccgggga aagtcggggg gaagatggcg
     5881 cgacagcgga gataacatgg ccgcccggct atccgtcgat acggcgttac gtgagtgcag
     5941 ggcgaaagga gagagatatg aaagcccatg acctggtgat cgccattctg gcggtcctcg
     6001 tcttccttct gatctcgctg ccctaccagt tcgcgtacag ctactgggca gagtccactg
     6061 cgctctgggt agtgtacatc gtggtaggtg ttatcctggc ggtgttcgtg ttcttcaact
     6121 ttctgcaaga ctggcgccgt ctcgtcgccg aagcggaagg tgaggagaaa aaggaggtgg
     6181 ccccatgatc gatgcacaag aaagcaagcg tcggaaagtc gggctaggcc tcctcagcat
     6241 agtggtcgta gtgtttatcg cattgagcgg gttgtccttc gtccaggaac gcggcaggcc
     6301 agaccccgaa atgatcctct ttcaagggta cagcgccgtg gatggcaagc gtgtctttca
     6361 ggcctacaac tgcatgggct gccacacgat gcttgggaac ggcgcctatt tcgggcccga
     6421 tctcaccaaa atctacgagg atggtggcgc agcgtggctg aaggctttcc tctctgcgcc
     6481 cggcacgtgg ccgaccaaag cggtggtgga cctgcgagtt gagcagctcc ggcagtctgg
     6541 cgatgtagat gtcgcctcca cggacgccta ctaccagcag tatccccacg tcctggaagc
     6601 cgtcgagctg tttggcggga agcgaacggt gatgccgaac ctcgtgttga agcccgatga
     6661 gatcgcggcg ctgaccgcct ttttcaacta tgcagccgaa atcgatacgg caggctggcc
     6721 gcccgcgcgc caggccgatg cggcggtagt agaacggctc caggctgagt ttggcgtcgt
     6781 accgccgacg gctgtcgaga ccgcgccgac gcctgccagt cctgtttcgt tggaggttca
     6841 gggtaagcag ttggccggcg aactcggttg ccttgcctgc cattccaccg atgggataca
     6901 gcgcagcggc tcgaccttca aaggcctctt tggagcaaca gtgcctttac aggatggcag
     6961 cacggtgact gt
//
LOCUS       DBNHXB010000444         8316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1650042, whole genome shotgun sequence.
ACCESSION   DBNHXB010000444 DBNHXB010000000
VERSION     DBNHXB010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1650042"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="AC162G_17510"
     CDS             complement(<1..1102)
                     /locus_tag="AC162G_17510"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5077656.1"
                     /translation="MLFSGTLGPTLLTGSLKTGSLAVMFDNVESLTCDRAGRLWSRWT
                     GGHTFRRGLDGRVLEKWQENGHRKRRWLLEVQAAPLLDESAARMRSLGESIAAGRVKW
                     IIAPEGDELAGVIERAAQFDARAAEHDRERFDAVYDRIGILPPDQYLSLVLQATTGCS
                     FNTCTFCDFYAAQRFRIKSDRAFRAHVRAVLAYMGESIRMRRSIFLGEANALTIPFNR
                     LVSLMTIVREEIGDHSPRSEGEWGWPVYAFLDAFSGTQKSVDEYRRLAALGLKRVSIG
                     LESGHDPLLDFVRKPGAAQDAVETVSALKMAGIAVSLIVLIGLGGNRYAEPHVSDTVA
                     VLNAMPLGKGDILYFSDLVEHESTPYPALSRAA"
     gene            1402..2331
                     /locus_tag="AC162G_17515"
     CDS             1402..2331
                     /locus_tag="AC162G_17515"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HIR5077657.1"
                     /translation="MKLIRNLIRAMRPKQWTKNLFVFIAVLFDQKLFMPGLFFSTPLG
                     RSTLGFILLCLLSGAVYLLNDIADAEQDRAHPTKRNRPIASGVLPLPVAWLAAIALPL
                     VCLALGFALDPGFGIALIVYWVNNIAYTFWLKHVVLLDVFSIALGFVLRVVAGVILVV
                     VVRFSPWLYVFTTLLSLFLGFSKRRGEIALLQGNAGNHRAILDEYSLPFLDNMMSIVV
                     AAASVTYSLYTFSAENLPVNHTMMLTIPFVLYGFFRYLYLVHLRGETMPPDEVLLRDR
                     GLQVTLVLYAVAVVLVFYQQPLLDLAARLTGAW"
     gene            2325..3278
                     /gene="mvk"
                     /locus_tag="AC162G_17520"
     CDS             2325..3278
                     /gene="mvk"
                     /locus_tag="AC162G_17520"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HIR5077658.1"
                     /translation="MVTASAPGKVILFGEHAVVYGRPAIAVPVTQVQATTTVEPALAG
                     RGATILATDLARVVRLSKANPGNPLASIVRLTLAHLGAPEPDANITVHSTIPIASGMG
                     SGAAVSASIVRSLARYLGRDVDDATLSSLVYEVEKLYHGTPSGIDNTVVAYQQPVYFV
                     RGAPVRIFTVKRPFTIAVGDTGVRSPTKIAVGDVRKAWEADRARYEALFDRIGSIVEQ
                     ARAAIEAGDVEALGPLMDANHALLREIGVSSDDLDRLVAAARRAGALGAKLVGGGRGG
                     NMIALIDAASQEAVRQALLDAGAKGVMTAKVEASSPRFGSA"
     gene            complement(3310..3555)
                     /locus_tag="AC162G_17525"
     CDS             complement(3310..3555)
                     /locus_tag="AC162G_17525"
                     /inference="COORDINATES: protein motif:HMM:NF027602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HIR5077659.1"
                     /translation="MDTPLSMLETVIVTSSFPDEQVLAGDLGTIVEVYTDPYLAYEVE
                     FVNPDGSTRALLTLLPEQVRKLLPTDILTTRQLPVMA"
     gene            3600..4421
                     /locus_tag="AC162G_17530"
     CDS             3600..4421
                     /locus_tag="AC162G_17530"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HIR5077660.1"
                     /translation="METVFLKLGGSIITDKTRAEQARVEVIRRLACEVRAAREARPDM
                     RLVMGHGSGSFGHAVATRYGTRDGVRDAAGWLGYAEVAASAARLNQIVTDVFVAERVP
                     VVSLPPSASARCEDGRLAHLDVEVFRALVRSRLVPLVYGDVAIDAVRGATIVSTEDVF
                     AYLANEAEPDRVLLAGEAAGVYPNADMTGEPIPLITPGTLEQFASALGGSRGFDVTGG
                     MAAKVRQMIGWVRAHPRTSARIFSAAEAGTLARVLIDPTAQIGTLIRTDAEAGHG"
     gene            4484..5047
                     /locus_tag="AC162G_17535"
     CDS             4484..5047
                     /locus_tag="AC162G_17535"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5077661.1"
                     /translation="MTISEDDYRVVDSQILVDHPSVRITMDTLEHNGQRRAHLILGGG
                     VAARAAIATVALTPDRQLVLTRQYRHALQAIIFDLPAGRLDPDEEPIEGARRELEEEA
                     GYRPGRLIHLGKFYQFPSTIDVPTHLFFADDLTATRQHLDEGEELEVVLMSVHEVLAM
                     IERGEVVDGSLQLGVLLAKHKGLLPEG"
     gene            5220..5432
                     /gene="rpsU"
                     /locus_tag="AC162G_17540"
     CDS             5220..5432
                     /gene="rpsU"
                     /locus_tag="AC162G_17540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR5077662.1"
                     /translation="MSFLTVTLRDEESFDGLLRRFNREVMNTGVMTDVRRKRFFVSKG
                     EQKRIDARKGRRRARIRQLRSQSREM"
     gene            complement(5499..6251)
                     /locus_tag="AC162G_17545"
     CDS             complement(5499..6251)
                     /locus_tag="AC162G_17545"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5077663.1"
                     /translation="MKALILAAGLGTRLGPLTRNIPKPMLPVGGRPLLEHIVELLRSH
                     GITEIALNLHYKPDAILNHFGSGDAWGVRLRYSYEAELLGSAGTALRHLAWFYPDPFL
                     VYYGDVYSDANLSDLMERHRFSGAAATVAVYQAPDPTRHGIVQFDTDGRVRSFVEKPP
                     ADRVLSNWANSGIYVLNPEVLHFVTGIPSDFEYDVFPRLLDAGQVIHAYPLTGTLIDI
                     GTPENYQVVQKLFPAQTTSSQSARLDITPAPA"
     gene            complement(6402..7523)
                     /gene="wecB"
                     /locus_tag="AC162G_17550"
     CDS             complement(6402..7523)
                     /gene="wecB"
                     /locus_tag="AC162G_17550"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HIR5077664.1"
                     /translation="MRIVSVVGARPDFIQAAPVSRALRRRHQEILVHTGQHYDYLMSQ
                     VFFEHLSLPEPDVNLEVGSGDHGQQTGEMVALLERTFRDMRPDLVIVRGDTNSALAGA
                     LAASKMHIPLAHIESGERSFNQRMPEEINCLVADRLADVLFCITRTAVRQLADEGITD
                     NVHYTGDVMLDALYQNLSVSRQRSDILSRLDLSTDRYLLATVHRAGNTDNPHNLRGII
                     AAFNALSEPVIFPVHPRTRGAIDRLGLAFGPHVRPIEPVGYLDMLALESNARVILTDS
                     GGVQREAYFLSIPCITLRDETGLMETVRTGWNRLAGADPERILTALRELVVPDEHPAL
                     FGDGHAAERIAEILTTHSIAFGQNYDRVVFDLVGQNVVA"
     gene            complement(7543..8025)
                     /locus_tag="AC162G_17555"
     CDS             complement(7543..8025)
                     /locus_tag="AC162G_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR/zinc-dependent alcohol dehydrogenase-like
                     family protein"
                     /protein_id="HIR5077665.1"
                     /translation="MTVSVNFIPEVKLSEAVDAADVAGLVKTLRRAQIELGDEVVVVG
                     DSPRARLAAGLAGIAGASCVRLVEPSALDEAPERSADVVIYALGETRWLSQALRITRD
                     LGRVLLLGPVGAIDLDVYPDLHKRSIRLIDVRPPDDPSPQTTEVVQHLIRSGYLQWGA
                     "
BASE COUNT         1485 a         2564 c         2745 g         1522 t
ORIGIN      
        1 ccgcagcgcg agagagggcc gggtacggcg tagattcgtg ctcgaccaga tcgctgaagt
       61 agaggatgtc gcccttgccg agcggcatgg cgttcagcac agcgacggtg tcggatacgt
      121 gcggctcggc gtatcgattg ccgcccaggc cgattaacac gatcaggctc acggcgatac
      181 cggccatctt gagcgcgctg acggtctcga cagcgtcctg cgcggcgccg ggcttgcgca
      241 cgaagtccag cagcggatcg tgtccgctct ccagcccgat actcacgcgc ttcagcccca
      301 gcgccgccag gcgccggtac tcgtcgacgg acttctgcgt gccgctgaag gcgtcgagga
      361 aagcgtacac cggccaaccc cactccccct cgcttcgcgg gctatggtcg ccgatctctt
      421 cgcgcacaat cgtcatcaag gagacgagcc ggttgaacgg gatcgtgaga gcattcgcct
      481 cgccgaggaa aatcgagcgg cgcatgcgaa tcgactcgcc catgtaggcg agcaccgccc
      541 gcacgtgtgc gcggaacgcc cggtcggact tgatgcggaa ccgctgcgcc gcgtagaaat
      601 cgcaaaatgt gcacgtgttg aacgagcagc ccgtcgtcgc ctgcagcacc agcgacaggt
      661 actggtcagg cggcaggatg ccgatccgat cgtagacggc gtcgaaacgc tcgcggtcat
      721 gctcggcggc tcgcgcgtcg aactgcgcag cgcgttcgat cacgccggcg agttcgtcgc
      781 cttccggcgc gatgatccac ttcacgcgcc cggcggcgat gctctcgccg aggctgcgca
      841 tgcgcgcggc gctctcgtcc agaagagggg cagcctgaac ctccaatagc cagcgccgct
      901 tgcgatggcc gttttcctgc catttttcga gcacgcggcc gtccagcccg cgccggaaag
      961 tatgcccacc tgtccagcgg ctccatagcc gcccggcgcg gtcgcaggtc agcgattcga
     1021 cgttgtcgaa catcacggcc agcgagccgg tcttcaagct gccggtcagc aaagtcggac
     1081 ccagcgtgcc gctgaacagc atcggctcac ctcagttcaa gctccgaagt tcaaactcca
     1141 aactgacacg ccgccggtgg cggcgcagtt accatcagat tagccaggcc cgtcacggac
     1201 agccagtgtc gcatggtaca taatgccggt gatagatgtc tcattcgacc cgttcgatcc
     1261 ggccggcacc ggccatcgat agccgccctg gacgatcgat atttattgtc aagattatct
     1321 ggaaaagccc gaaggcgcat ggaattggag cgatttgcgt cctgtggtat attgcgcgca
     1381 gttctgcggc gaggaacgac cttgaagctg attcgcaatc tcatccgggc gatgcgcccc
     1441 aagcagtgga cgaagaacct gtttgtgttc atcgcggtgc ttttcgacca gaagctgttc
     1501 atgccggggc tgtttttctc cactccgctg gggcgatcga cgctggggtt tattctgctg
     1561 tgcctgctgt ccggcgccgt gtacctgctt aacgacatcg ccgacgcaga gcaggatcgg
     1621 gcgcacccga ccaaacgcaa ccggcccatc gcgtccggcg tgctgccctt gcctgtcgcg
     1681 tggcttgccg cgatcgccct gccgctcgtg tgcctggcgc tcggcttcgc gctcgatccc
     1741 ggtttcggca tcgccctgat cgtgtattgg gtcaacaaca ttgcgtacac attctggctg
     1801 aaacacgtcg tgctcctgga cgtgttcagc atcgcgctgg gatttgtgct gcgcgtcgtg
     1861 gcgggcgtga tcctcgtcgt cgtcgtgcgc ttctcgccgt ggctgtacgt gttcaccacg
     1921 ctgctgtccc tgttcctggg cttctcgaag aggcgtggcg agatcgccct gctgcaaggc
     1981 aacgccggca atcaccgcgc catcctcgac gaatacagcc tcccctttct cgacaacatg
     2041 atgagcattg tcgtcgcggc ggccagcgtg acctactcgc tctatacctt ctccgccgag
     2101 aatctgcccg tcaaccacac gatgatgctc accatcccct tcgttcttta tggcttcttc
     2161 cgctatcttt acctggtgca cttgcgcggc gagacgatgc cccccgacga agtgctgctg
     2221 cgcgaccggg gcttgcaggt cacactcgta ttgtacgctg tggccgtggt gctggtgttc
     2281 tatcagcagc ctctcctcga tttggcggcg agattaacgg gagcatggta actgcctcgg
     2341 cgcctggcaa ggtgatcctg ttcggcgagc acgccgtcgt gtacgggcgc ccggcgatcg
     2401 ccgtgccggt gacgcaggtg caggcgacga cgacggtcga gccggccctc gccgggcgcg
     2461 gcgcgacgat cctggcgaca gacctggccc gcgtcgtgcg cctgtccaaa gccaacccgg
     2521 gcaatccact ggcctcgatt gtgcgcttga cgctcgcgca cctcggcgcg cccgagcctg
     2581 acgctaacat cacggtccat tcgacgatcc ccatcgccag cggcatgggc tccggcgcgg
     2641 cggtgagcgc gtcgatcgtg cggagtctcg cgcgctacct cgggcgcgac gtcgacgacg
     2701 cgacgctgtc gtcgctcgta tacgaggtcg agaagctgta tcacggcacg ccgagtggga
     2761 tcgacaacac cgtcgtggca taccagcagc ccgtgtattt cgttcgcggc gcgccggttc
     2821 gaatcttcac ggtgaagcgc ccgttcacca tcgcggtcgg cgataccggc gtgcgcagcc
     2881 cgacgaagat cgccgtcggc gacgtgcgta aggcatggga agcggaccgg gcgcgctacg
     2941 aagcgctatt cgaccggatc ggatcgatcg tggaacaggc acgcgcggcg atcgaagcgg
     3001 gcgacgtcga ggcgctcggg ccgctcatgg acgcgaacca cgcactgctg cgcgaaatcg
     3061 gcgtgtcgag cgacgatctg gatcggctgg tcgcagcagc caggcgcgcg ggcgcgctgg
     3121 gcgcgaagct cgtcggcggc gggcgtggcg gcaatatgat tgctctgatc gatgcggcgt
     3181 cacaggaggc cgtccggcag gcgttgctcg atgccggggc gaagggtgtg atgacggcga
     3241 aggtcgaggc ctcatcacct cgtttcggga gcgcgtgatg aaggcacgac gaggagaatg
     3301 gccgcgccct caggccatga ccggaagttg gcgggtggtc agtatgtcgg ttggcagaag
     3361 cttgcgcacc tgctctggca acagtgtcag cagggcgcga gtcgagccat cggggttgac
     3421 gaactcgact tcgtaagcca gatacggatc agtgtatact tcaacaattg tgccgagatc
     3481 gccggccagt acttgctcat cagggaagct ggatgtaaca atgaccgttt caagcataga
     3541 tagcggtgtg tccatgacta cgtttccttt tgccgcgacg ccgattcaat tataacagaa
     3601 tggaaaccgt ttttctgaaa ctcggcggct caattatcac cgacaaaaca cgtgcggaac
     3661 aggcacgcgt ggaagtgatt cgccgcctgg cgtgcgaggt gcgcgcggcg cgtgaggccc
     3721 gccccgacat gcgcttggtg atgggacacg gcagcggatc gttcggtcac gccgtggcga
     3781 cgagatacgg cacgcgcgac ggcgtgcgcg atgcggcggg atggcttggc tacgccgaag
     3841 tcgccgcgtc ggcggcgcgc ctgaatcaga tcgtcaccga cgtattcgtc gcagagcgcg
     3901 tgcccgtcgt cagcctgccg ccgtcggcca gcgcgcgctg tgaggacggc cggctggcgc
     3961 atctcgacgt ggaggtcttt cgcgcgcttg tccgcagcag actcgtgccg ctcgtgtacg
     4021 gcgatgtggc gatcgacgcc gtgcgcggcg cgacgatcgt gtcgactgag gacgtgttcg
     4081 cctatcttgc caatgaggct gagcctgatc gcgtgctgct agctggcgaa gcagccggcg
     4141 tgtatccgaa cgcggacatg accggcgaac cgatcccgct catcacgccg ggcacgctcg
     4201 agcagtttgc atcggcgctg ggcggatcgc gtggcttcga cgtgacgggg ggcatggccg
     4261 cgaaagtgcg ccagatgatc ggatgggtgc gcgcgcatcc gcgcacgtcg gcgcgcattt
     4321 tctcggcggc cgaagctggc acgctggcgc gcgttctgat cgacccgaca gcgcagatcg
     4381 gcacactcat tcggaccgac gcggaggcag gacacggatg aacatggatt tcacggatag
     4441 tggcctctca acgtccgtgt cgtccgtggc aggaggctgc gctatgacga tttctgagga
     4501 cgattatcgt gtggtggatt cgcaaattct cgtcgatcat ccaagcgtgc gcatcacgat
     4561 ggacacgctg gagcacaacg gccagcggcg cgcgcatctg atactcggcg gcggggtcgc
     4621 ggcgcgcgcg gcgatcgcca cggtcgcgct cacgcccgac cggcagctcg tgctcacgcg
     4681 ccagtaccgg catgcactgc aagccatcat cttcgatttg cctgccggcc gcctggatcc
     4741 agacgaggag ccgatcgaag gtgcgcgccg tgagctggaa gaggaggccg gttaccggcc
     4801 gggacggctc attcacctgg ggaagttcta tcagttcccc agcacaatcg acgtgccgac
     4861 gcatttattc ttcgccgacg atctgaccgc gacgcgccag catctcgacg aaggcgaaga
     4921 actggaagtc gtgctcatgt ccgtccacga agtgctggcg atgatcgagc gcggcgaagt
     4981 cgtcgacgga tcgctgcagt tgggggtctt gctggcgaag cacaaggggc tgctgcctga
     5041 aggttagacg gaatcgtgcc aggggatgtg cgtggagctg aagggacgcg ggcgacgagc
     5101 agaaacggtg tgggccagag cagcacatgg gagttgacgg cgccggtccg ccgtggtatc
     5161 attcgcgcgc actgaggaat tatcgttgcg ctcttgaagc gcacgtgtgc aggaaagggg
     5221 tgagtttctt gacggtcacg cttcgcgacg aagagtcgtt cgacggcctt ttgcgccggt
     5281 tcaaccggga agtgatgaac accggcgtga tgaccgacgt ccgccgcaag cgcttcttcg
     5341 tgtccaaggg cgagcagaag cgcatcgacg ctcgcaaagg ccgccggcgc gcgcgcatcc
     5401 gccagttgcg ctcgcagagc cgcgagatgt aagactaaga aacccggttt ctcagagaaa
     5461 ccgggtttct tgtttgtcac ccctctcctc tggcccactc aagcaggcgc cggcgtaatg
     5521 tcgaggcgcg ccgactgaga tgaagtggtc tgcgcaggga acaacttctg aacgacctga
     5581 tagttttcgg gcgtgccgat gtcgatcagt gtgcccgtca gcgggtaggc gtggatcacc
     5641 tggcccgcat ccaggagccg cgggaatacg tcgtattcga aatcagaggg aatgccggtc
     5701 acgaagtgca gcacttccgg gttgagcacg tagatgcccg agttggccca gttggataac
     5761 actcgatcgg caggcggttt ctcgacgaag cttcgaacgc gcccgtccgt atcgaactgg
     5821 acgatgccgt ggcgcgtcgg atcgggcgcc tggtagacgg cgacggtcgc ggcggccccg
     5881 ctgaaccggt ggcgctccat caagtcgctc aggttggcgt ccgaatacac gtcgccatag
     5941 tacacgagga agggatccgg atagaaccag gcgagatgac gcagcgctgt gcccgccgag
     6001 cctagcagct cggcttcgta ggagtagcgc agccgtactc cccacgcgtc tcccgagccg
     6061 aagtgattga gaatcgcgtc cggcttatag tgcaggttga gcgcgatctc ggtgatgccg
     6121 tgagagcgca gcagctcgac gatgtgctca agcagcgggc ggccgccgac cgggagcatg
     6181 ggcttgggga tgtttcgggt aagcgggccg aggcgggtgc ccagcccggc cgccaagatg
     6241 agcgccttca tccgtagacc cctgtctttt tcaacatggc cggtttcgtg cgcgcgctgc
     6301 tatcgccggt gagagccgcc gcttgctcgt gttcaatcgt catggagtcg ctccgtgtaa
     6361 ggctataggt tctcgggtct ccagcgcagc cctgcgagag gctaggcgac gacgttctgc
     6421 ccgacgagat cgaacacgac ccggtcgtaa ttctggccga aggcgatcga atgcgtggtc
     6481 aagatctcgg cgatgcgctc ggcggcgtgg ccatccccaa agagcgccgg atgctcgtct
     6541 ggcacgacga gctcacgcag cgcggtcaga atgcgttccg gatcggcccc ggcgaggcga
     6601 ttccagcctg tgcgcacggt ttccataagc ccggtttcat cgcgcagcgt gatgcagggg
     6661 atcgacagga agtacgcctc ccgctggacg ccgccggagt cggtgaggat gacgcgagcg
     6721 ttgctttcca gcgccagcat gtccagatag ccgaccggct cgatcggccg cacgtgcggc
     6781 ccgaacgcca ggccgagccg gtcgatcgcg cctcgcgtgc ggggatgcac agggaaaatc
     6841 accggctcgc tcaaagcatt gaaggcagcg attatgccgc gcaggttgtg cgggttgtcg
     6901 gtgttgcccg cgcggtgcac ggtcgccagc agataccggt cggtcgacag gtcgagccga
     6961 gacaggatat ccgatcgctg ccgggaaacg gacagattct ggtagagcgc gtcgagcatc
     7021 acatcaccgg tgtagtgcac gttgtcggtg atgccttcgt cggccaactg gcgtaccgcc
     7081 gtgcgcgtga tgcaaaacag cacgtcggcc aggcggtcag ccaccaggca gttgatttcc
     7141 tccggcatgc gctggttgaa cgagcgctca ccggactcaa tgtgggccag cgggatgtgc
     7201 atcttggaag ccgccagcgc gcctgccagc gccgaattcg tgtcgccgcg gacgatgacg
     7261 aggtcaggcc gcatgtcgcg aaacgtgcgc tcaagcaacg ccaccatctc gcccgtctgc
     7321 tggccatgat cgccggaccc gacttcgaga ttgacgtccg gctcgggcag gctcaggtgc
     7381 tcgaaaaata cctgcgacat gagatagtcg taatgctgcc cggtatgtac caggatttcc
     7441 tggtggcgac gccgcaacgc ccggctgaca ggcgcggcct ggatgaaatc gggccgcgcg
     7501 ccgacgacgg acacgatcct catatctcac cctcttcgtg tgttacgcac cccattgcag
     7561 gtagccggac cggatcaggt gttggacgac ctcggtcgtc tggggcgacg ggtcgtcagg
     7621 cggcctgacg tcgatcaggc ggatcgacct cttgtgcagg tcagggtaca cgtctaagtc
     7681 tatggcgccg acggggccga gcagcaggac ccgacccaga tcgcgcgtga tgcgcagcgc
     7741 ctggctgagc cagcgcgtct cgccgagtgc gtagatcacg acgtcggcgc ttctctcggg
     7801 cgcctcgtct aaggccgacg gctcgacgag cctgacgcac gacgcgccgg cgatgcccgc
     7861 caggccggct gccagccgcg cgcgggggct gtctccgacg acgacgacct cgtcccccag
     7921 ttcgatctgc gcccggcgta acgtcttcac gagaccggcg acgtccgctg cgtcgacggc
     7981 ttcgctcagc ttaacctctg gaatgaaatt gacgctgact gtcatctgtc ccctacctcc
     8041 accgatacat tccatgcacg ccgcgagcgg gtgatgaaac gattcatgcg gcaaggccag
     8101 atcggcgctg acctgccttg ggcgacggat gctcagggga tcaaggctgg catagggatg
     8161 ccagcatact gtcccgacaa gaactaggcg aacacacgtc agttagatga caggatgagt
     8221 cgggttagaa gaaagtataa actggagaca gataggtctt tagcggtttt tcgagacgtg
     8281 tgggaggaag gtggggaagg tcggcacgta gttttt
//
LOCUS       DBNHXB010000445         2479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3496725, whole genome shotgun sequence.
ACCESSION   DBNHXB010000445 DBNHXB010000000
VERSION     DBNHXB010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3496725"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="AC162G_17560"
     CDS             <1..328
                     /locus_tag="AC162G_17560"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR5077666.1"
                     /translation="IWLTVPVADQPLRLPALPLTWLALAWLGLLGSCLAYLLYFYLLN
                     AWGATRAALVTYVFPVIGLILGIVFLQETADWRLVAGSLLVVGGIAIVNFKPRLKPAS
                     AAMATD"
     gene            339..1253
                     /locus_tag="AC162G_17565"
     CDS             339..1253
                     /locus_tag="AC162G_17565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR5077667.1"
                     /translation="MTRRYPFVQVDVFTDRAFAGNPLAVFADARGLSAGEMQTLAREM
                     NFSESTFVLPPETPNAVKRVRIFTPGKELPLAGHPTVGTAYVLARQGAFDLRDGATEV
                     TLQLGIGAVKVAIEAQDHEPRFVWMEHREAEFGAVRDDRERVASALGISPEDIDADYP
                     LQVVSTGAPFLFVPLRSLAAAGRCRSNTSALAALPGDVGEGVYVFTTETVSREASIHV
                     RMFAPHTLDIPEDPATGSGAAPLGAYLARYGLVSNAPETRFICEQGIEMGRPSRIHVD
                     VRRDGERITRLRIGGQAVIVGEGDLFWE"
     gene            1258..2004
                     /locus_tag="AC162G_17570"
     CDS             1258..2004
                     /locus_tag="AC162G_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR5077668.1"
                     /translation="MPSRRSEFFSGVKAELPILLGTFPFGILYGVLALDAGLPPALAF
                     AMSSIVVAGSSQVIAAGLFKSATPAIVIILTTLVVNLRHMLYSTSVAPHVKHLSRVWK
                     WLLAYLLTDEAYAVAITHYNRSESGATHLTHKHWYFLGAGLTLWATWQASTALGIFVG
                     TQIPGSWGLDFTVALTFIALVVPVLKDRPGVAAFAAAGIAAVAGAGLPYKLGLIAAAF
                     IGIGVGLWLETRQAGALHLHPTPASEAQYE"
     gene            1997..2332
                     /locus_tag="AC162G_17575"
     CDS             1997..2332
                     /locus_tag="AC162G_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIR5077669.1"
                     /translation="MSDAELWLTVAGMGVVTFAMRLLPIALLERVSLPALVQRGLRFV
                     PPAVLSAIIFPELLVRDGALDVSPGNTRLIAGLVAALVARATRNVIVTIVVGMATLWL
                     LQAIWQIPE"
BASE COUNT          367 a          780 c          836 g          496 t
ORIGIN      
        1 gatctggctg accgtgcctg ttgccgatca gccgctgcgc ctgccggctt tgccgctcac
       61 ctggctcgcg ctggcgtggc tcgggctgct ggggtcgtgc ctggcatact tgctgtactt
      121 ctacctgctc aacgcctggg gcgcgacacg cgccgcgctg gtgacttacg tgttcccggt
      181 aatcggcttg atactgggga tcgtcttttt gcaggaaacg gcggactggc gtctcgtcgc
      241 cggctcgctg ctcgtggtcg gcggcatcgc catcgtcaac ttcaagccgc gtttgaagcc
      301 ggcgtccgca gccatggcta cggattaagg agtcacacat gacccggcgc tatcccttcg
      361 ttcaggtcga cgtgttcacc gatcgcgctt tcgccggcaa cccactggcc gtgttcgccg
      421 acgcgcgcgg cctgagcgcc ggcgagatgc agacgctcgc gcgcgagatg aacttctctg
      481 agtccacttt cgtcctgccg cccgaaaccc cgaacgccgt gaagcgcgtg cgcatcttca
      541 cgccgggcaa agagctgccg ctggccggcc atcccaccgt cggcacggcc tacgtgctgg
      601 cccggcaggg tgcgttcgac ctgcgcgatg gggcgaccga agtcacgctg cagctcggca
      661 tcggcgcggt gaaagtcgcc atagaggcgc aagaccatga gccgcgcttc gtgtggatgg
      721 agcatcgtga ggcggagttt ggcgcggtgc gtgacgatcg cgagcgagtc gcctcggcgc
      781 tcggcatttc gcccgaagac atcgacgccg attatccgct gcaagtcgtg tcgaccggtg
      841 cgccgttttt gtttgtgccg ctgcgctcgc tggctgcggc cggccgctgc cgatcgaata
      901 cctccgccct cgccgccctg ccgggtgatg tgggtgaggg ggtgtacgtg ttcactaccg
      961 agaccgtgtc gcgcgaggca agcatccacg tgcggatgtt tgcaccgcac acgctggaca
     1021 tccccgaaga cccggcgaca ggcagcggcg cggcgccgct gggtgcgtac ctggcgcgct
     1081 acggtttggt gtcgaacgcg ccggagacgc gcttcatctg tgagcagggc atcgagatgg
     1141 gccgccccag ccggattcac gtcgacgtgc gccgcgatgg agagcgcatc acccgtctgc
     1201 gtatcggcgg gcaggcggtg atcgtcggcg agggcgacct cttctgggag taatgccatg
     1261 ccgtcacgac gctccgaatt cttctccggc gtgaaagcgg aactgccgat cctgctcggc
     1321 acgttcccgt tcgggattct gtacggcgtg ttggcgctgg atgccgggct gccgccggct
     1381 ctggcatttg ccatgtcgtc gatcgtcgtc gccggctcgt cgcaggtgat cgccgccggc
     1441 ctgttcaaaa gcgctacgcc tgcgatcgtg ataatcctga cgacgctcgt cgtcaacctg
     1501 cggcacatgc tgtatagcac gtcggtcgcg ccgcacgtca agcacctgag ccgggtatgg
     1561 aagtggctgc tggcctacct gctgaccgat gaagcctacg ccgtggcgat cacgcactac
     1621 aaccgatcgg aatcaggtgc gacgcacctc acacacaagc actggtattt tctgggcgcg
     1681 gggctgacgc tgtgggcgac ctggcaggcc agcacggcgc tcggcatttt cgtcggcacg
     1741 cagattccgg gcagttgggg cctggatttc accgtggcgc tgacgttcat cgccctggtc
     1801 gtgcctgtgc tgaaggatcg accgggtgtg gctgcgtttg cagcggccgg catcgccgca
     1861 gtggccggag ccgggctgcc gtacaaattg ggcttgatag cggcggcgtt catcggcatc
     1921 ggcgttgggt tgtggctgga gacgcgccag gccggcgcac tgcatttgca tcctactccg
     1981 gcgagcgagg ctcaatatga gtgacgcgga actgtggctg accgtcgccg gtatgggcgt
     2041 ggtgaccttt gctatgcgcc tgctaccgat cgcgctgctg gagcgagtga gcctgccggc
     2101 gctcgttcaa cgcgggctgc gtttcgtgcc gccggccgtg ctgtcggcga tcattttccc
     2161 cgagctgctg gtgcgcgacg gcgcgctcga cgtgtcgccc ggcaacacgc gcctgatcgc
     2221 cggcctcgtc gcggcgctgg tggcgcgggc cacacgcaac gtgatcgtga ccatcgtggt
     2281 cgggatggcg acgctgtggc tgctgcaggc gatttggcaa attccggaat agaggcttta
     2341 gccggttcga ttatccgcca catctccggc taaagcctcg agtccagccg aagatatggg
     2401 cgcttgccaa agtccagttt ataataatgc gtgtgaatga gattgccggg aggtgtaagt
     2461 ctatgctgtc cactcctaa
//
LOCUS       DBNHXB010000446         7868 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3497012, whole genome shotgun sequence.
ACCESSION   DBNHXB010000446 DBNHXB010000000
VERSION     DBNHXB010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3497012"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..762
                     /locus_tag="AC162G_17580"
     CDS             163..762
                     /locus_tag="AC162G_17580"
                     /inference="COORDINATES: protein motif:HMM:NF013212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR5077670.1"
                     /translation="MTDNVHEQVLDATSGDGEVQPTEIASGQAQAAEAGAPEAPAAEG
                     EEIDLGTEIERLKAEAAANLDGWQRALAEFTNYRRRNEAERSQLVFLTGVKIMEKMLP
                     VIDDFDRALANLPEGLQSNGWVEGVRLTRNKLIGILESEGLSIIPVAPGDPFDPAVHE
                     AVTHEASDDFAEGRIIAEVQKGYRIGDRVLRPSLVRVAR"
     gene            973..2856
                     /gene="dnaK"
                     /locus_tag="AC162G_17585"
     CDS             973..2856
                     /gene="dnaK"
                     /locus_tag="AC162G_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR5077671.1"
                     /translation="MGKIIGIDLGTTNSVVAVMEGGEPVVISSSEGSRLFPSVVAINP
                     KTGERLVGQVARRQAIVNPENTVFSVKRLMGRKFKDAEVQKAIKRLPYKIIEAPNGDC
                     RVIMGGKEYSPPEISAMILQKIKQDAEAYLGETITQAVITVPAYFNDSQRQATKDAGK
                     IAGLEVLRIINEPTASSLAYGLDRKKDETIAVYDLGGGTFDISILDVGDGVFEVKSTN
                     GDTYLGGDDFDERVIDWVADEFKKDQGIDLRVDRQALQRLKEAAEKARIELSSLMETE
                     INLPFITADATGPKHLQMKFTRARLEQLTGDLIERSIEPCRRALEDAKASPADIDEVV
                     LVGGMTRMPAVQEAVKSFFSKEPHKGVNPDEVVGVGAAIQAGVLGGEVKDVLLLDVTP
                     LTLAIETLGGVATALIQRNTTIPYRKSQIFSTASDGQTSVEIHVTQGERSMAADNKTL
                     GKFILDGIPPAPRGMPQVEVTFDIDANGIIKVSAQDKATGRSQHITITASSGLSEQEV
                     EKMVKEAQQHAAEDQKSRERIEARNAADSAVYTAEKTLRELGDKAPADIKSDVEAKAN
                     ALKGMLETASAEELKQRTQELHEALQRIGAAMYQQPGEGPSTPPPGGSEGEDVVEGEF
                     SKN"
     gene            2860..3978
                     /gene="dnaJ"
                     /locus_tag="AC162G_17590"
     CDS             2860..3978
                     /gene="dnaJ"
                     /locus_tag="AC162G_17590"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR5077672.1"
                     /translation="MATTKRDYYDVLGVGRGASGEDIRRAYRKLARQYHPDVNKDGGA
                     EARFKEINEAYEVLSDERKRAAYDRFGHAGAEGGFSTDFGGFGGFSDIFEEFFGGFGT
                     RTARRGPRRGADLRYDLTIDFVEAAFGVEKEIDVTRHELCPRCRGTGAEPGTTPVRCA
                     TCNGTGEVRRMQQSILGSFVNVTTCPTCRGEGETVPIVCIECRGSRYVRLGRTLTVKI
                     PPGVDTGTQIRLAGEGEPGERGGPSGNLYVVLDIKPHPYFRRKDDDVVLEVALNVAQA
                     VLGDRVTIPTLDGDEAIDIPPGTQPGKVIRMRGKGIPHLRRSGRGDQLVVVQVSIPTS
                     LSADQRRMFTELSKTLGREVIAQNDKGFLDKLKNLFSI"
     gene            4195..5250
                     /gene="prmA"
                     /locus_tag="AC162G_17595"
     CDS             4195..5250
                     /gene="prmA"
                     /locus_tag="AC162G_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR5077673.1"
                     /translation="MNWLEISIAVDAEAAEAVAEVLSRYAPNAVALEQRARDIQDAPA
                     CPRSAAEGGSGWSPDGPLEPTVVVRAYLPVDAGVDAKRQQAAEALWHLRQIVPMPDPV
                     FREIAPGDWETAWKEHYHVLRVGPRIVVKPSWREHTAEPGDVVIELDPGMAFGTGLHP
                     TTQMCLKAVETRCRPGMQVLDLGTGSGILAIAAAKLGASSVIALDTDPVAVESARDNV
                     RRNAVDHVVSVEAGSLAEIQSRRLAFDLALVNILAKTIVQLCDEGLAEAINPGGQIVC
                     AGLIESQEDEVREALERAGLEVIERFQDKDWVGLACRPATRPRALSAGARAAAYRSSG
                     TTWPARNRNVMRPLFRS"
     gene            complement(5181..5426)
                     /gene="infA"
                     /locus_tag="AC162G_17600"
     CDS             complement(5181..5426)
                     /gene="infA"
                     /locus_tag="AC162G_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR5077674.1"
                     /translation="MAKEEKITVEGTVIEALPNTMFKVKLDNGHEVLGYLSGKMRRHY
                     IRILLGDRVKLELSPYDLNKGRITFRFRAGQVVPLDR"
     gene            complement(5589..6812)
                     /locus_tag="AC162G_17605"
     CDS             complement(5589..6812)
                     /locus_tag="AC162G_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077675.1"
                     /translation="MDLSIVFPMFWGREKKEVFKEDDHFYDHPTALDDEGTIPPLFES
                     LRILRDRAFDIIAVAGANHPSLAQAVEKRAGELLRKYASQAGVTLHYFSYSHLQRLHD
                     YLRELGKGDLLETISLVGYSPLRNACLVAAHILGKDIAVSIDDDCLFIEPGYIGRIKA
                     KMLSDFEGQPIRAYCGPYITARDTIYLDRPASPHAVYWNVVEVMNQAFRQFIVESPGI
                     KEVPFAIMGNIAVHRDFYTRCPLDPPLRRGEDMDWVMNAHILGERFILDTELVIKHDP
                     PPRPYPTWRPLREDIYRFRYQQAKIANSRDGDGYHAVNRERYLPYPGAFFQDDFLARV
                     TQACTILAIDYLTKNEPDNARESLNNITHAHSLAIPDEDPFQSFLAFQKKWEEMMGII
                     EERRPEVQKRVFGDV"
     gene            complement(6867..7499)
                     /locus_tag="AC162G_17610"
     CDS             complement(6867..7499)
                     /locus_tag="AC162G_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HIR5077676.1"
                     /translation="MKKIDLRKELKHLYAPSAKKVEVVNVPKFSFVMMDGQIEPGETP
                     ETSQAFRDAMMALYGVSYTLKFMSKLRKRNPIDFTLMALEGLWWGDAGEFDFGKKATW
                     KWTMMIMQPKHITEKMVQEALRQLQEKKDNPALSRMRFEPFHEGLCLQIMHLGPYSEE
                     PRTIERMKAFAEENGYIYRGKHHEIYLGDPRRAKPEKLRTILRHPVEKVA"
BASE COUNT         1586 a         2384 c         2474 g         1424 t
ORIGIN      
        1 atggacgcgc ggtgagcacc gtgcgctacg tgtcccgcct gctcagcgac ctggtgtccg
       61 acatggcgca gggctaggag attatctcac agcgaaacgt agtggcgaca gggtttgaat
      121 tttgaagttt gaggtttcga gtttgaagtg aggtgatgac gggtgactga taacgtgcac
      181 gagcaggtgc tggacgcaac gtcaggcgac ggggaggttc aacccacgga gatcgccagc
      241 ggccaggcgc aagccgccga agcgggcgcg cctgaagcgc cggccgctga gggtgaggag
      301 atcgatcttg ggacggaaat cgagcgactg aaagcggaag ccgccgccaa cctggacggc
      361 tggcagcgcg cgctcgccga gttcacgaac tacaggagac gcaacgaggc cgagcgcagc
      421 cagctcgtgt ttctcaccgg cgtcaagatc atggagaaga tgcttccggt gatcgacgac
      481 ttcgatcgcg cgctggccaa cttgccggag gggcttcaaa gcaacggttg ggtcgagggc
      541 gtccgcctca cgcggaacaa gctgatcggc atactcgaaa gcgaaggcct gtcgatcatt
      601 ccggtcgcgc ccggcgaccc gttcgacccc gccgtgcacg aagccgtcac gcacgaagca
      661 tcggacgatt tcgcggaagg gcgcatcatc gcagaggttc agaaaggcta tcgcatcggc
      721 gaccgcgtgc tgcgaccgtc gcttgttcgc gtcgcccgct aaaacctatc cgaaaacccc
      781 gtagcatcgg gccttgtgcc cgcgcccacg aggggctaag ctacgttttc ggataggccc
      841 taaacaagcg ctcatatctt cggctggact cgaggcttta gccggaggtg tggcggataa
      901 tcgaaccggc taaagcctct attccggagt ttgccaaact ccagttgtaa ggagacagga
      961 gagacattgg ctatgggaaa aattatcggc atcgatttgg gcacgacgaa ttcggtcgtg
     1021 gcggtgatgg aggggggaga gccggtcgtc atttcatcca gcgagggcag ccgcctgttc
     1081 ccgtcggtcg tcgccatcaa tccaaagacc ggcgagcgcc tggtgggtca ggtggctcgc
     1141 cgccaggcga tcgtcaatcc cgagaacacg gtcttctccg tgaagcgcct gatgggccgc
     1201 aagttcaagg acgcggaggt gcagaaagcg atcaagcgcc tcccctataa gatcatcgaa
     1261 gcgccaaacg gcgactgtcg cgtcatcatg ggcggaaaag agtactcgcc gccggagatc
     1321 tcggccatga tcctccagaa gatcaagcaa gacgcggagg cgtatctcgg cgagacgatt
     1381 acccaggcgg tgatcaccgt gccggcctac ttcaacgaca gccagcgcca ggcgaccaag
     1441 gacgccggca agatcgccgg gctggaagtg ctgcgcatca tcaacgagcc gacggcgtcc
     1501 tcgctggcct acggcctcga ccgcaagaag gacgagacga tcgccgtgta cgacctgggc
     1561 ggcggcacgt tcgacatctc gatcctcgac gtcggcgacg gggtattcga agtcaagtcg
     1621 accaacggcg atacgtacct gggcggcgac gatttcgacg agcgcgtcat cgattgggtg
     1681 gccgacgagt tcaagaaaga ccagggcatc gacctgaggg tggatcggca ggcgctgcag
     1741 cgtctgaagg aagccgccga aaaggccagg atcgagctgt ccagcttgat ggaaacggag
     1801 atcaatcttc ccttcatcac cgccgacgcg acaggcccca agcaccttca gatgaagttc
     1861 acacgcgcca ggctcgagca gctcaccggc gacctgatcg agcgctcgat cgagccgtgc
     1921 cggcgtgcgc tcgaagacgc caaggcgtcg ccggccgaca tcgacgaagt cgtcctggtg
     1981 gggggcatga cccgtatgcc ggccgttcag gaagccgtca agagcttctt cagcaaagag
     2041 ccgcacaagg gcgtcaatcc tgacgaggtc gtcggcgtcg gcgcggccat ccaggccggc
     2101 gtgctgggcg gcgaagtgaa ggacgtgctg ctgctcgacg tgacgccgct caccctagcg
     2161 atcgagacgc tgggcggcgt ggcgacagcc ttgatccagc gcaacacgac gatcccgtac
     2221 cgcaaatctc aaatcttctc gacggcgagc gacggccaga ccagcgtcga gattcacgtg
     2281 acgcaaggcg agcggtcgat ggcggccgac aacaagaccc tgggcaagtt catcctggac
     2341 ggcattcccc ctgccccgcg cggcatgccc caggtcgaag tcacgttcga tatcgacgcc
     2401 aacggcatca tcaaagtctc ggcgcaggac aaggccaccg gccgcagcca gcacatcacg
     2461 atcacggcgt cctccggcct gagcgagcag gaagtcgaga agatggtgaa ggaagcgcag
     2521 cagcacgcgg ccgaagacca gaagagccgc gagcgcatcg aggcgcgaaa tgcggccgac
     2581 tccgccgtgt acaccgccga gaagacgctg cgcgaattgg gcgacaaggc gcctgctgat
     2641 atcaagagcg acgtcgaagc gaaggcgaac gcgctgaagg gcatgctcga aacggccagc
     2701 gccgaagaat tgaagcagcg cacgcaggag ctgcacgagg cgctgcagcg catcggcgct
     2761 gcgatgtacc aacagccggg tgaaggccct tcgaccccgc ctcccggtgg ctctgaaggc
     2821 gaagacgtcg tcgagggcga attctcaaag aactgatgaa tggccacgac taagcgcgat
     2881 tactacgacg tcctgggggt tgggcggggc gccagcggcg aagacatcag gcgcgcgtat
     2941 cgcaagctgg cgcgccagta ccatcccgac gtgaacaagg acggcggcgc cgaagctcgc
     3001 ttcaaagaga tcaacgaagc gtacgaagtc ctctcggacg agcgcaagcg cgccgcgtat
     3061 gaccgctttg gacacgccgg cgcggagggc ggtttctcga ccgacttcgg aggcttcggc
     3121 ggtttcagcg atatcttcga agagttcttc ggcgggttcg gaacgcggac ggcgcggcgc
     3181 gggccgcgcc ggggcgccga cctgcgctac gatctgacga ttgacttcgt cgaagcggcg
     3241 ttcggggtcg agaaggaaat cgacgtcacg cggcacgagc tatgcccgcg ctgccgtgga
     3301 accggcgccg agccgggcac tacaccggtg cgctgcgcca cgtgcaacgg cacgggcgaa
     3361 gtccgccgca tgcagcagag catcctcggc tcgttcgtca acgtcacgac ctgccccacc
     3421 tgtcgaggcg aaggcgaaac ggtgccgatc gtctgcatcg agtgccgggg ttccaggtac
     3481 gtccgcctcg gcaggacgct cacggtgaaa atcccgccgg gcgtagacac cggcacgcag
     3541 atccggctgg cgggcgaagg cgagccgggc gagcgcggcg gcccatctgg aaacctctac
     3601 gtcgtcctgg acatcaagcc gcatccgtac ttccggcgca aggacgacga cgtggttctc
     3661 gaagtcgcgc tgaacgtggc gcaggccgtt ctgggtgaca gggtcactat tcccacactc
     3721 gacggcgacg aagcgatcga catcccgccg ggcacgcagc cgggcaaggt catccggatg
     3781 cgcggcaagg gtatcccgca cctgcggcgc agcggccggg gcgatcagct cgtcgtcgtg
     3841 caggtcagca ttccgaccag cctcagcgcc gatcagaggc ggatgttcac ggagctgtcc
     3901 aagacgctcg ggcgcgaagt catcgcgcag aacgacaagg gctttctgga caagctgaaa
     3961 aatctcttca gtatttgaca cacggatttc ccacggatta cacggaatgg caggtattgc
     4021 cgcccctgtc tgtcaacgaa tgggggaacg aataaacgaa tgacctatgg ccgctggcgt
     4081 tatgcgttta ttcgttggac attcgttgac agagttactt gccttttgcg ctcccgctgc
     4141 atctgtggcc tactgatcac tgatcactga tcactgatta ctgattacgg gcctatgaac
     4201 tggcttgaga tcagcatcgc cgtcgatgca gaggcagccg aggctgttgc cgaggtgttg
     4261 tcgcgttacg cgccaaatgc cgtggcgctt gagcagcggg cgcgagatat tcaggacgcg
     4321 cccgcctgcc ctcgtagcgc agcggagggg ggctccggct ggtcgcctga cggcccactc
     4381 gaaccgaccg tcgtcgtgcg cgcctacctg cccgtcgacg ccggcgtcga cgcgaagcgg
     4441 cagcaggccg ccgaggcgtt gtggcacctg cgccagattg tccccatgcc cgatcccgtc
     4501 tttcgagaga tcgcgccggg cgactgggag actgcctgga aggaacatta tcacgtgctg
     4561 cgcgtcgggc cgcgtatcgt cgtcaagccg tcgtggcgcg agcacacggc agagccgggc
     4621 gacgtcgtca tcgagctgga tcccggcatg gccttcggca cgggcttgca ccccacgacc
     4681 cagatgtgcc tgaaggcggt cgagacgcgc tgccgcccgg gcatgcaggt actcgacctc
     4741 ggaacaggct cgggcatcct ggcgatcgca gccgcgaagc tcggcgcgtc gtcggtcatt
     4801 gcgctcgaca ccgatcccgt cgccgtcgag tcggctcgtg acaacgtgcg ccgcaacgcg
     4861 gtcgaccacg tcgtgagcgt ggaggccgga tcgctggcgg aaatccagag ccgtcgcctg
     4921 gcgttcgacc tggcgctggt caacatcctg gcgaaaacga ttgtccagct ctgcgatgaa
     4981 gggctggctg aagcaatcaa ccccggcgga cagatcgtct gcgcggggtt gatcgaatcg
     5041 caagaagatg aagtgcgcga agcgctcgag cgcgccgggc tagaagtgat cgaacgattt
     5101 caagataagg actgggtcgg gcttgcctgc cggcctgcca cccgccctcg cgcgctgtct
     5161 gcgggggcgc gcgcagccgc ttaccgatcg agcggcacga cctggcccgc gcggaatcgg
     5221 aacgtgatgc gacccttgtt cagatcgtag ggagacagct cgagcttcac acgatcgccg
     5281 agcaggatgc gaatgtaatg gcggcgcatc ttgcccgaca ggtatccgag cacctcgtga
     5341 ccgttgtcta gcttgacctt gaacatggta ttcggcagcg cttcgatgac ggtgccttcg
     5401 acagtgatct tctcctcttt cgccatcctc tctcctcgct tgagctggac tttggcaagc
     5461 gcccatatct tcggctggac tcgaggcttt agccggagat gtggcggata atcgaaccgg
     5521 cctctattcc ggaatttgcc aaactccagg tcgagttgaa agcagtatac cgcatcacgg
     5581 ctgtccggtc aaacgtcgcc gaacactctc ttttgcactt ctggccgccg ttcttcgatg
     5641 atgcccatca tctcctccca cttcttctgg aacgccagga acgactggaa cggatcttcg
     5701 tccgggatcg ccagagagtg ggcgtgggtg atattgttga gcgactcgcg ggcattgtcc
     5761 ggctcattct tcgtcaaata atcgatggcc agaatcgtac aggcctgcgt cacgcgcgcc
     5821 aaaaaatcat cctggaagaa ggcgccggga tagggcagat agcgctcccg gttgactgca
     5881 tgatagccgt ccccgtcccg gctgttggcg atcttggcct gctgatagcg aaaccgatag
     5941 atgtcctccc gcagcgggcg ccaggtgggg tacgggcgcg gtgggggatc atgcttgatg
     6001 accagctcgg tgtccaagat gaagcgctcg ccgagaatgt gcgcgttcat gacccaatcc
     6061 atgtcttcgc ctcggcgcag gggtgggtcg agcgggcagc gcgtgtagaa gtcgcgatgc
     6121 acggcaatgt tgcccatgat ggcgaacggg acttccttga tgcccggcga ctcgacgatg
     6181 aactgtctga aagcctggtt catgacctcc acgacgttcc agtacaccgc gtgggggctt
     6241 gccgggcgat cgagatagat cgtatccctg gccgtgatgt aggggccgca gtaggcccga
     6301 ataggctgtc cctcgaagtc cgacagcatc ttcgctttga tccgcccgat gtacccaggc
     6361 tcgatgaaca ggcagtcgtc gtcgatcgac acggcaatat ccttgcccag gatgtgggcg
     6421 gccaccagac aggcgttccg cagcggcgag tagccgacga gcgaaatggt ctcgagcaga
     6481 tctccctttc ccaactcgcg gagatagtcg tgcaggcgtt gcagatggga gtagctgaag
     6541 taatgaagcg tcacgccggc ctggctggca tatttgcgca gcagctcgcc ggcccgcttc
     6601 tcaaccgcct gcgccaggga ggggtggttg gcgccggcaa cggcgatgat atcgaacgcc
     6661 cgatcccgca aaatcctcag gctctcgaag agcggtggaa tcgtgccttc gtcgtccagc
     6721 gccgtcgggt gatcataaaa atggtcgtct tctttgaaaa cttccttctt ttctctaccc
     6781 cagaacatcg ggaaaacgat ggacagatcc atgcccgcca cctccctgtt gattcacgag
     6841 cgcgtcatta cccccgccct cgacctctaa gcaaccttct caaccggatg ccgcagaatc
     6901 gtccgtagtt tctccggctt ggctcgtcgc ggatcgccca ggtaaatctc atggtgttta
     6961 ccgcgataga tatagccgtt ctcctcggca aaggccttca ttctctcaat cgtccggggt
     7021 tcttccgaat acggaccgag gtgcatgatt tgaagacaca atccttcgtg aaagggttca
     7081 aaacgcatcc gggataaggc cgggttatcc tttttttcct ggagttgccg caacgcctcc
     7141 tggaccatct tctccgtgat gtgcttcggc tgcatgatca tcatggtcca tttccatgtc
     7201 gccttcttgc cgaaatcgaa ttcaccggca tccccccacc acagcccttc gagcgccatc
     7261 agggtaaagt cgataggatt gcgtttgcga agcttcgaca tgaacttcag cgtataggac
     7321 acaccgtaca atgccatcat ggcatctcgg aaggcctggg atgtctctgg cgtctcgccg
     7381 ggttcaatct ggccgtccat catgacgaaa ctgaacttcg gcacgttgac gacctcgact
     7441 ttcttcgccg acggcgcata caggtgcttc aattcctttc ttagatcgat cttcttcatg
     7501 gtatcctccc ttatgaacag cccgttcaag gcaagctaca gagatgcggc ggggcaggcg
     7561 gcgcttcgtc cacccgatgc taagtttaga gcaactcgga aattaagcca agtcgcaggc
     7621 gcatctcctc aatcatgcgt cctcgtaaag cggtcatggc tatgcctcct gccgaggcga
     7681 cgcatttttg tggtatcatg cgcgcgtttg ctcagaacgc atcggccggc gtcgatgcgc
     7741 tataccggcc acacatgaaa atgccgaacc ggagtgccgg ctttagccgg cttggctgag
     7801 caggctgaag ccctgatctt tacccacatt tgtcagctgg tgtcgggagc gcggaacagg
     7861 cgcgcccc
//
LOCUS       DBNHXB010000447         6745 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2323131, whole genome shotgun sequence.
ACCESSION   DBNHXB010000447 DBNHXB010000000
VERSION     DBNHXB010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2323131"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="AC162G_17615"
     CDS             complement(<1..209)
                     /locus_tag="AC162G_17615"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010637613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR5077677.1"
                     /translation="MSDNKHIIDVNETTFQAEVVERSKKIPVLVDFWAPWCGPCRVLS
                     PTLERVAADSDGAFVLAKVNTDENP"
     gene            complement(459..1535)
                     /locus_tag="AC162G_17620"
     CDS             complement(459..1535)
                     /locus_tag="AC162G_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077678.1"
                     /translation="MFKDRDLQNQLRDLAHWLLLAPVALLLLFGCGQLALLGASRPSA
                     ADTHSKLRADYSPWPFTVIGAVRNDIVDAIRRDEGVDPILVPDSIWPASPPPVDEPAA
                     TPTAPPAATPTLTLTPGQASTASPMPTSRSSLTATAGAPTATWKPTVTLTPSPIPTDT
                     PPPTSAPSNTPGPIQTPVPSSTPAFIPTATSTPSHTPTPSSTPTLLPMQVSPRVDCIV
                     DNGDGTFVAFFGYDNPNTFAVTIPIGSNNYFVPSPGDRGQPTTFAPGAYQRVFSVSSS
                     GIALVWHLDGNTDVASFGTVLCPGTPTPTQTPAPTPTSTPTPTDTTRPTDTPTPTDTP
                     TPSDTPTPTDMPTPTDTPTPTSTP"
     gene            complement(1540..3048)
                     /locus_tag="AC162G_17625"
     CDS             complement(1540..3048)
                     /locus_tag="AC162G_17625"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding response regulator"
                     /protein_id="HIR5077679.1"
                     /translation="MEQEKILVVDDDESLRYLMVTHLRRRGYTVESARDGLDALQVLQ
                     TDGPFTVLVTDLMIPNMNGFQLLREARRLDPRLEVIVITGVATVKSAIAAMREDGAFD
                     YLTKPLETIGELSMAVARAITYRNLQLEREALQAQLSAEAERLQLLIANIGDAILSAD
                     ASGALTVVNPAAIRLLGRDDLVGSDALTALPQPLATVVANWQAVGDQRPAVVEVDWPA
                     GSTQMVSLTPMGAKGSRSHAWVMAVRDITHLKQLDELKMRILTEMIGKIQLPLAQAMA
                     TLTQLDGTAADNGRRAAEVVHRLVNEWRRIQDWTGDMLALVRYESSFGIKTTEVDLSA
                     VLNETLRYLLEGPPLRAKELTVNLSLSPDLPAVYTDAGLLRKLLHGLISRAALRSQQG
                     GELRVSASARSRHVWIEVGDQGPPVQESDVPRIFEKSFVEPNAVSGGTDLELALIRAI
                     VDKAGGQVWVRGQGSLGSTIAFCLPRAGEDNGSSESASAACRTDAIELSTAK"
     gene            complement(3060..5018)
                     /locus_tag="AC162G_17630"
     CDS             complement(3060..5018)
                     /locus_tag="AC162G_17630"
                     /inference="COORDINATES: protein motif:HMM:NF026177.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5077680.1"
                     /translation="MALSQKGLGRVLDEAPVTGLHRRSIRSVRLPKLSSLRAKLIVPY
                     VVLTLLIAMVGTYVVTRLVTSSIRERFVNQLYETSRVAADGIVRRERTNLADLRLMAY
                     TEGVHQAMSDRDAAGLQDHLWPLVLNNNVEAVTAIDLNGHEIITLARDPVSGQYLFST
                     GADFSEVGLVRNVLDGQVDSAGDKFVELLETERGLYLFTSAPVRDSTDTLVGALMVGT
                     RLQTLLVELKSQALADIVVLDRHGNLMATTLVQPDEGYSALEMTPQAVADVDPSQTRD
                     VDLYGRNFQMVYAPLEVREQTLGLLGVVLPSSYLVTTEATSRDTFSLLFSLVTLVVVL
                     LGYGLSQSIARPILRLQAILQAVAAGDLNQRTGLNRSDEIGALATAFDSMTLRLRERT
                     EEAARLYAESVQRNRELADINARLQETQQHLIQSEKLAAIGQLTAGIVHDVKNPLMVI
                     KGLAETLQEDTDLDPETQEQLDVIRDSAAQANQIVTDLLKFARQSMLEMSQQDLASTV
                     ESALRLTAYLVQRAGVEVVADLPAQPVMVIYDAQQIEQVLINLIQNAIQAMPDGGTLR
                     VSLRQAGEAVAIAVQDTGVGIPPEKLTRVFDPFFTTKPDGEGTGLGLSVSYGIVSHHK
                     GRIDVESTVGQGTTFTILLPTRQPLAAS"
     gene            complement(5349..6689)
                     /locus_tag="AC162G_17635"
     CDS             complement(5349..6689)
                     /locus_tag="AC162G_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5077681.1"
                     /translation="MNDRREIFGWSMYDWANSAFSTTVVTVFLGPYLTGLIESQVGEG
                     GAFSLLGIPIAAESFFAYCVSASVFLQVFILPLLGAIADYSHLKRRMMLLFATIGAVT
                     TFGMFFITGDLYVLGGLMFILANVAFGASIVFYNAFLPQIASPDQRDRVSSFGWAMGY
                     LGGGLLLALNLAFLLFVAPALNVSRGDAARISIASAGVWWLVFGYFAIGRLRERGAQR
                     VLPPGENYLSVGLKQLGHTIGELRKHPTALKYLLAYLLYNDGIQTVIAISAVFGDQEL
                     GMELSELTLLILMVQFVAFIGALLFGRIAGRLGAKRSIILSLLIWSSISIWAQVSLQS
                     KIEFWVLGGVVGIVLGGSQALSRSLFSQMIPKEREAEFFSFYEISERGTSWTGPLLFG
                     LVTQATGSMRTAILALIVLFASGLLILASVDVKRAVRESGNEETPEVGLAPATP"
BASE COUNT         1296 a         2043 c         2096 g         1310 t
ORIGIN      
        1 ttcgggttct cgtcggtgtt caccttcgcc aggacgaacg cgccatcgct gtcggcagcg
       61 acgcgttcaa gagtggggct taagacgcgg cacggcccgc accacggcgc ccagaaatcc
      121 accaggacag ggattttctt cgagcgctcg accacttcgg cctgaaaggt tgtttcgttc
      181 acatcgatga tatgcttgtt gtcactcatt cttgtgccct tcgatacgat tcgccgggta
      241 ttttaccaga aatggggacg agttggcgag tcagcgagtc agcgaatcag cgggcgtatt
      301 cgttgattcg ttgcccattc gatgcaccag atagacaacg gataaacgga tgcgccgatc
      361 ggcatccggt tatccgtttt gcatccgttg cctctgtgcc ctccctcgaa ggcattgaac
      421 gcgggtgaac accggttgtg gcagatgtca acccatggtc agggtgtgct ggtcggcgtg
      481 ggtgtatcgg tcggcgtggg catatccgtc ggcgtgggtg tgtcggatgg cgtgggcgtg
      541 tcggtcggcg tgggtgtatc ggtcggcctg gttgtatcag tcggcgtcgg tgtgctggtc
      601 ggtgtcgggg caggtgtttg cgttggtgtg ggcgttcccg ggcagagaac cgtaccaaac
      661 gacgcgacgt cggtgttgcc gtcgagatgc cagaccaggg ctataccgct ggagcttaca
      721 ctgaaaaccc tttgataggc acccggcgcg aacgtcgtcg gctgaccacg atcaccgggg
      781 ctggggacaa agtagttatt cgacccaatg ggaatcgtga ccgcgaatgt atttggattg
      841 tcatagccga aaaaggcgac gaaagtgcca tcgccgttgt cgacgatgca atccactctt
      901 ggggaaactt gcattggcag gagcgtaggc gtgctgctgg gcgtcggtgt atgtgacggc
      961 gttgaggtgg ccgtcgggat gaatgccggt gtgctggaag ggacaggcgt ttgaatcggc
     1021 ccaggggtat tgctgggagc cgaagtgggc ggcggtgtat cggttggtat ggggctgggc
     1081 gtgagtgtga cggttggctt ccacgttgca gtcggtgcgc cggcggttgc cgtaagcgat
     1141 gatctcgacg taggcatggg cgaagctgtc gaagcctggc cgggtgtaag tgtgagagtc
     1201 ggtgtcgcag ccggcggagc ggttggcgta gccgccggct cgtccacagg cgggggcgat
     1261 gccggccaga tcgaatcggg cacgaggatc ggatcgacac cctcatcgcg acggatcgcg
     1321 tcgacgatgt cgttcctgac ggcgccgatg acggtgaacg gccacgggct gtaatcggcc
     1381 cgcagcttgg aatgggtgtc ggcagcggac gggcggctcg ctcccagcaa ggccagttga
     1441 ccacaaccga acagaaggag cagcgccacc ggcgcgagca gcagccagtg cgctaaatcg
     1501 cgtagctgat tctgtaagtc cctgtctttg aacaatgggt cacttcgctg tgctcagttc
     1561 aatcgcatct gtgcgacacg cggcgctggc ggactcagaa ctcccattgt cctctccggc
     1621 tcgcggcaga cagaaggcga tggtgctgcc aagggaaccc tggccgcgca cccagacttg
     1681 cccgcctgcc ttgtcgacga tcgctctgat caaggccagt tccaggtcgg tgccgccgct
     1741 gaccgcattc ggttccacga acgacttctc gaaaatgcga ggcacgtcgc tctcctgaac
     1801 cggcggtccc tgatcgccga cttcgatcca gacgtgtctg ctgcgcgcgc tcgcagacac
     1861 acggagctcg ccaccctgct ggcttctcaa agcggcccgg ctgatcaacc catgcaggag
     1921 cttgcgcagc aggcctgcgt cggtgtagac cgcaggcaga tcgggagaca ggctcagatt
     1981 tactgtgagt tccttcgcac ggaggggtgg cccttctaat agatatctta aggtttcgtt
     2041 caagacggct gaaaggtcaa cctctgtcgt cttgatgccg aaactcgact cataccgcac
     2101 cagcgccagc atgtcgcctg tccaatcctg gatgcgccgc cactcattga ccagacgatg
     2161 caccacctcg gccgcgcgcc ggccgttgtc cgctgctgtg ccgtccagct gggtcagggt
     2221 ggccatggcc tgcgccaagg gaagctgaat cttgccgatc atctcggtca agatgcgcat
     2281 cttcaattcg tccaactgtt tcaggtgcgt gatgtcgcgc acggccatca cccaggcgtg
     2341 ggatcgactg cctttcgcgc ccatcggcgt caggctaacc atctgtgtcg aaccggccgg
     2401 ccaatcgact tcgacgacgg ccggtcgttg gtccccaacc gcctgccagt tggcaacgac
     2461 ggtggccaga ggttgcggaa gagcggtcaa cgcatcgctg cccaccaggt cgtcccggcc
     2521 cagcaggcgg atcgcggccg gattgacgac ggtcagggcg ccgcttgcat ctgcggacag
     2581 aatcgcatcg ccgatgttgg cgatcaggag ctgcagccgc tcggcctcgg cgctcagttg
     2641 agcctgcaac gcctctcgct ccagttgcag gttccgatag gtaatggctc gcgcgaccgc
     2701 catcgacagc tcgccgatcg tttcaagcgg tttggtcagg tagtcgaagg ccccatcctc
     2761 gcgcatggcg gcaatcgccg atttcaccgt cgccacgccg gtgatgacga tcacttccag
     2821 ccgtggatcc aaccggcggg cctcgcgcag caattgaaaa ccgttcatgt taggtatcat
     2881 gagatcggtc accaggacgg tgaaaggccc atcggtttgc agaacctgga gggcgtcgag
     2941 gccatctcgc gcgctctcga cggtgtaacc gcgtcgccgc aggtgggtca ccatcaagta
     3001 acgcaggctc tcatcatcat cgaccaccaa tattttttcc tgctccacgg ctgatctcct
     3061 taagaggcgg caagaggttg ccgggttggc aacaggatgg taaaggtcgt accttgcccg
     3121 accgtgctct cgacatcgat gcgtcccttg tgatgcgaga cgatgccgta gctcaccgac
     3181 agacccagcc cggtcccttc cccgtccggt ttcgtcgtaa agaagggatc gaaaacccgg
     3241 gtcaatttct ccggcgggat cccaacgccg gtgtcctgaa cggctatcgc caccgcttca
     3301 ccagcctgac gcaaactcac acgcagggtg ccgccgtccg gcatcgcctg gatcgcattc
     3361 tgaatcagat taatcagcac ctgctcgatc tgctgagcat cgtagatgac catcacgggt
     3421 tgtgccggca ggtctgcgac cacttcgacg ccggctctct ggaccaggta tgccgtcaat
     3481 cgcagcgccg actcgaccgt ggatgccaga tcctgctgac tcatctcgag catggactgc
     3541 cgcgcgaatt tcagtagatc ggtgacgatc tgattcgcct gcgctgcact atcgcgaatc
     3601 acgtcgagtt gttcctgcgt ctcgggatca aggtccgtat cctcctgcaa tgtctccgcc
     3661 agccctttga tcaccatcag agggttcttg acatcgtgga cgatacccgc cgtcaattgc
     3721 ccgatggcgg ccagtttctc ggattgaatc aggtgctgtt gagtctcctg cagccgcgcg
     3781 ttgatatcgg ccagctcccg gttgcgctgt accgattcgg catacaggcg ggcggcttcc
     3841 tcagtgcgct cacggagacg cagcgtcatg ctgtcgaagg ccgtcgccaa cgcaccgatt
     3901 tcatccgacc ggttgagtcc ggtgcgctga ttgagatcgc cggccgctac ggcctgcaat
     3961 atggcttgca gccgcaagat aggccgggcg atgctctgcg acagcccgta gccgagcagg
     4021 acgacgacca gggtcaccag tgagaagagc aggctgaacg tatcccggct cgtcgcctcg
     4081 gtagtgacga ggtaactgct gggcaaaact acgcccagaa ggcccagcgt ctgctctcgc
     4141 acttccagcg gggcgtagac catctgaaag tttcgaccgt agagatcaac gtcgcgcgtt
     4201 tgcgaaggat cgacgtcggc gaccgcctgc ggggtcatct ccagggcgct gtacccttcg
     4261 tccggttgga ccagggtggt ggccatcaga tttccgtgcc ggtcgagcac gacgatgtcg
     4321 gcgagggctt gtgacttcaa ctcgaccaga agcgtctgga gacgtgtgcc gaccatcagt
     4381 gcaccgacaa gcgtgtcggt cgagtcccgg acgggggcac tggtaaagag gtataggccg
     4441 cgctcggtct ccaacagttc gacgaatttg tcaccggctg aatcaacctg gccgtcaagg
     4501 acgttgcgga ccaggccaac ctccgagaag tctgctcctg tagagaacag gtattggcct
     4561 gacaccggat cgcgcgccag ggtgatgatc tcatgtccat tcagatcaat cgctgtcacg
     4621 gcttccacgt tgttgttcag caccagcggc cataggtgat cctgcaagcc ggctgcatcc
     4681 cgatccgaca tcgcctggtg aacgccctcg gtgtaagcca tcaggcgcag gtcggccaga
     4741 ttcgtccgtt cccggcggac gatcccatcc gcggccaccc ggctggtttc gtagagctgg
     4801 ttgacgaagc gctcccggat cgaggaggtc accagtcgcg tgacgacgta ggtgccgacc
     4861 atggcgatca gcagagtcag cacgacatag gggacgatca gtttcgcccg cagactcgag
     4921 agtttcggca gccgcacgga tcggatcgac cggcgatgca ggccagtcac tggcgcttca
     4981 tccaacacgc gccccaatcc tttctgactc aatgccattt caatcccctg acgacccgaa
     5041 accggcgctc taacgcagaa tcggccggct tttaggacgc gtgcgtcacc cgctgcgcta
     5101 tgccctttgc agccagctcc ctgagaccat tgtagcggaa tgccatcaaa tcaacgttgg
     5161 cggtgggatg aggtttggtg tggtctgggt tgggattgca tttggttgaa gtgacatgat
     5221 ttagggtgcg gaggcaactg gcggagtggc gggggtgtca acgaatgagc aatgaatcaa
     5281 cgaataaacg gatgcgccga tcgccatccg tttatccgtt ttgaatccgt tgccgcgtcc
     5341 caaacttctt acggcgtcgc aggcgcaagc cctacctccg gcgtctcctc gttgccgctc
     5401 tcgcgcaccg cgcgcttcac gtcgaccgac gccaggatca acaggcccga ggcaaacaag
     5461 acgattaacg ccaggatcgc cgtgcgcatg ctgccggtcg cctgcgtcac cagcccgaac
     5521 agcagcggcc ctgtccacga cgttccgcgc tcgctgattt cgtagaacga gaagaattcg
     5581 gcctcgcgct ctttcgggat catttgcgag aacagcgagc ggctgagcgc ctggctgccg
     5641 ccgagcacga tgccgacgac gccacccagc acccagaact cgatcttgct ctgcaggctc
     5701 acctgcgccc aaatgctgat gctcgaccag atcaggaggc tgagaatgat cgagcgcttc
     5761 gcgcccaggc ggccggcgat gcggccgaac agcagtgcgc cgatgaaggc gacgaactgc
     5821 accatcagga tcaacagcgt cagctcgctc aattccatgc ccagctcttg atcgccgaac
     5881 acggcggaaa tcgcgatgac cgtctgaatg ccgtcgttgt agagcaggta ggccagcagg
     5941 tatttcagcg cggtcggatg cttgcgcagc tcgccgatgg tatgccccaa ctgcttgagg
     6001 ccgacgctca ggtagttctc gcccggcggc agcacgcgct gcgcgccgcg ctcgcgcagc
     6061 cggccgattg cgaaatagcc gaagacgagc caccacacac cggccgaggc gatgctaatg
     6121 cgcgcggcgt cgccacggct cacgttgagc gcgggcgcga cgaacagcag gaatgccagg
     6181 ttcagtgcca acaggagtcc gccgcccagg taacccatcg cccacccgaa ggacgacacg
     6241 cggtcgcgct gatccgggct ggcgatctgc ggcaggaacg cgttatagaa tacgatcgac
     6301 gcgccaaaag ccacgttggc gaggatgaac atcaggccgc ccagcacgta caggtcgccg
     6361 gtgatgaaga acatgccgaa cgtcgtgacg gcgccgatcg tcgcgaacaa cagcatcatg
     6421 cgtcgcttca ggtgtgaata gtcggcgata gcgccgagca gcggcagtat gaagacctgc
     6481 aggaacactg aggcagacac gcagtaagcg aaaaacgact cggccgcgat cggaattccc
     6541 agcagcgaaa acgcgccgcc ttcgcctacc tgcgactcga tcaggccggt gagatacggc
     6601 cccagaaaca ccgtcaccac cgtcgtcgag aaggccgagt tggcccagtc gtacatcgac
     6661 cagccgaata tctctcgcct gtcgttcatg gtttccccct ggtagacaat gccgagatgg
     6721 tcgtgggatg ctgccaagtg tattc
//
LOCUS       DBNHXB010000448         9211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2827089, whole genome shotgun sequence.
ACCESSION   DBNHXB010000448 DBNHXB010000000
VERSION     DBNHXB010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9211
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2827089"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1508)
                     /locus_tag="AC162G_17640"
     CDS             complement(<1..1508)
                     /locus_tag="AC162G_17640"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077682.1"
                     /translation="MTDSSRPHNRYSHSLAAARSLVASLAIALSALIVLGGLLTAAQP
                     VGALAAPLSQVPARTGSAASLTGVGRGEGRVSAPPFSPPAAPLLQASTSLAIAIHSTP
                     FATIDSNDPISSPVVWAVEAIITNTGPTTATAPFVDLDMNPTASWFLLPGESRLRNLP
                     PLASSSSQAVYWFARRSNSPDDPHTYTITAWASNANTVTQQLNAYDPFSPTVQTRAAL
                     NTGNNGIVSVGANVQVGAAFTMSVDYDMGTNARGVTVQPVGNSSFDPASYRLLGNEVQ
                     FSDGTNPVGPIYRDRLYFPQGSLPAGARRVRTTYTFVALRPANVTLCPYTDVRYNTQN
                     KFDNGFCAGNAAVPITGTLSFSFTKSVNAATVRQGELLTYTLRYTNTGDLPISNVYVW
                     DNIPTQTITSSVSPAADGFSTPDRVVWNIGTIGQSGTPTSIGEVMLTVLVNGRGSELL
                     DGTSLVNNGHMGILFNPSAVNPAVTSTVTTIVEAPTVAISKSDDQDTVIQGQV"
     gene            2064..2603
                     /locus_tag="AC162G_17645"
     CDS             2064..2603
                     /locus_tag="AC162G_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077683.1"
                     /translation="MSQQSHDIVEPSARSDRRNLMVAGAALIAIGLLAFAAQFIEFVD
                     VGLLFLPTLGVIFLVWGIAIRRVGPMIPGGILTGIGVGVILMEQTAALPGEASDAGIF
                     LITFGAGWALITLLSALFTPKTQWWPLIPGAIIAAVGAALFAGDAGLRVLEFAGDFWP
                     LVLIAAGLIIVLRAVRSAR"
     gene            complement(2726..3319)
                     /locus_tag="AC162G_17650"
     CDS             complement(2726..3319)
                     /locus_tag="AC162G_17650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077684.1"
                     /translation="MKLYSFAIVVVAALAVGGLLGFSLGNGSEATGVNVAQPDAVAPS
                     SAVEAPVQVPVEAPVGAVGVLQAVSAEGLSLETREGLQFMTITGPAAGIEQLKVGDKV
                     AVWAQKVNGQLAVKQIAVVPETPQRMHYLGLVANSSADRIELVGQQGETTSFRIDAAT
                     QRMPDASRAPQVGDTVTVVAKPDPLGDGWLAVAIVTR"
     gene            complement(3580..4170)
                     /locus_tag="AC162G_17655"
     CDS             complement(3580..4170)
                     /locus_tag="AC162G_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077685.1"
                     /translation="MRTLLIVVLVILVVYLLYSNGTLSRLIPGLPDPKVTPQILVTLV
                     PPVRIDPLAGVTTPSPAEPTETPISSPTSTPVIVDVATPLPAQPTLPPPTIIVPEIPP
                     TLAVSTTPTPASIFPLTIDTPRDGETVSASPWLVVGQTQPGALVSVNDAVGFANDEGR
                     FSLPAALLPGVNVLEVIASNTAGEEVFVILTVVYQP"
     gene            4296..5414
                     /locus_tag="AC162G_17660"
     CDS             4296..5414
                     /locus_tag="AC162G_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077686.1"
                     /translation="MHIVHIDAADRRRVKQFLDLPFRLYRSTPEWVPPLSSDARRVFD
                     RKRHAFYQHSEAAFFLAMNGERAAGRIVAIDNRLYNEFNKERTAFFYLFECEEDRAAS
                     QSLFDAAFDWARSRGLDKVIGPKGFTALDGMGLLVKGFEHRPAFGIPYNLPYYPALVE
                     AAGFEPGGDVVSGYLSTKMPFPEKIHQVAEIVQKRRGLRVARFKSRRDLLALLPRLQA
                     MYNDSLGGTTGNTPLTDADMRALVNQLYWFADPKLIKIVMKGEEPVGFSLAYPDISAA
                     MQRTQGRLLPFGWIDMLLELRRTKWVNLNGAGIVEKYRGLGGTALLFSEMHKSVVEGG
                     FEHADIVQIGVDNDKMQRELRDLGIGFYKTHRLYRRAI"
     gene            complement(5469..6326)
                     /locus_tag="AC162G_17665"
     CDS             complement(5469..6326)
                     /locus_tag="AC162G_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077687.1"
                     /translation="MAGASKPTPKDTVRALNRLSAEQFARVCSEIGYLEVTLPGDTQT
                     AKASALVGKLRGEADFAVLVRAMNRVNPGAWRAVPVRGSLSSAVYGVLAFAVVFGIGG
                     LVMALILSGIEPGAGPIPTATETLAPTRTPIPTFTYTPTPTSLPTETATPTPTATSPR
                     RAVSTVPVQAPTETSTAPPVSIVYREVEPLKPPAGSRYYSGETVEFRWVLRDASIQAD
                     ERYLMRLYLNGAIVDSYLTPDPWRYYPVPDGAKGEFAWTVTVVKVDAAGNVIGPLSPE
                     SDPVMVVWQ"
     gene            complement(6467..7315)
                     /locus_tag="AC162G_17670"
     CDS             complement(6467..7315)
                     /locus_tag="AC162G_17670"
                     /inference="COORDINATES: protein motif:HMM:NF014679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR5077688.1"
                     /translation="MPSRIRVVTDSTAVLPPEFVAAHSIEVIPQIVRFGEAAFLEDVE
                     LSYGDFIRRLKSSGQLPKTSAPPPGAFVDAYARLLTEAQTILSIHPSLDVSATVQSAL
                     TAKGDSFPDADIRVIDTRTVAGNLATLVMLAVEWAESGVEVDEIVSRLQAMIPRGRVY
                     FLVATLEYLQKGGRIGGASALIGSTLQIKPILEIKNGHVEAKEKVRTHHRAFERLKDL
                     AIEQCPRSSGAHLCVMHADDLDEAQRLAGDLKPALGIERIPIYGVGASITTHAGPGTL
                     AVGFFV"
     gene            complement(7343..7615)
                     /locus_tag="AC162G_17675"
     CDS             complement(7343..7615)
                     /locus_tag="AC162G_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HIR5077689.1"
                     /translation="MDEPSAAPEEKGQYRKEEFKVTGEQLLSEIKRLVSEGNIRRIVI
                     RQGDKTLIEIPLTLGVIGIALAPVWAAIGAIAALVAECTIVVEKVE"
     gene            complement(7649..8446)
                     /locus_tag="AC162G_17680"
     CDS             complement(7649..8446)
                     /locus_tag="AC162G_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077690.1"
                     /translation="MRWRSGAVFWAIILIVLGAIFLLQNFGILSVGWDVIFPLLLIGL
                     GGWLLLASLVRRGASAAVSNSVPLEGARQARIEVNHGAGELRIGPSDDPAVLVSSTSD
                     GGVISDVRRVDDRLDVRLREDRDWWFWMWPGHWGAPYRWSMAIYRDVPVALDVHTGAS
                     KAGIDLRDVKVTALSVEVGASSVDLTLPASGQVSARIKAGAADVKVRVPDGMALRVQS
                     NVGAATLNVRGQRFQWGSNTYQSPDYGTNPNRTDLGIEGGAATITVM"
     gene            complement(8798..>9211)
                     /locus_tag="AC162G_17685"
     CDS             complement(8798..>9211)
                     /locus_tag="AC162G_17685"
                     /inference="COORDINATES: protein motif:HMM:NF024773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR5077691.1"
                     /translation="LEGDTCYASLRDLPRTPDGVIVSLPPNKVTPVLRDAADLGIRNV
                     WLQQGAESPEALALGKELGLSVAHGACVLMYMQPVRSFHGWHRAAARLFGRVEPTDKV
                     TPQMLAERRPKTADPRAMDLLDDEAAELLRNTQVK"
BASE COUNT         1651 a         2916 c         2948 g         1696 t
ORIGIN      
        1 acctggccct gaatgacagt gtcctgatcg tcactcttgc tgatggcaac ggtcggcgcc
       61 tccacaatcg tggtgacggt cgacgtgacc gccgggttca cggcactcgg gttgaacaag
      121 atgcccatgt ggccgttgtt caccagggac gtgccgtcca gaagttcgga gccacggcca
      181 ttgaccagta cggtcagcat gacctctccg atcgaggtcg gcgtgccgct ctgcccgatc
      241 gtgccgatgt tccacacgac gcgatccggc gtgctgaagc catcggccgc gggcgacacg
      301 gacgacgtga tcgtttgggt cgggatgttg tcccaaacgt acacattcga aataggcagg
      361 tcgccggtgt tggtgtagcg cagcgtgtaa gtgagcagct caccctgccg caccgtcgcc
      421 gcgttcaccg acttggtgaa gctgaaagac agtgtcccgg taatcggcac cgccgcatta
      481 cccgcgcaga agccattgtc aaacttgttc tgggtgttgt agcgcacgtc ggtgtacgga
      541 catagcgtga cattggcagg gcgcagcgcg acgaaagtat atgtagtcct gacgcgccga
      601 gcgccggccg gcaaactgcc ttgcgggaag tacagtcgat cgcgatagat ggggcctacg
      661 gggttcgttc catcgctgaa ctgtacttca ttgccgagca gccgatagga agccggatcg
      721 aagctggaat tgcccacggg ctgaacggtc acgcctcgcg cattcgtgcc catgtcgtag
      781 tcgaccgaca tcgtgaaggc cgcgccgact tgcacgttgg cgccgacgct gacgatgccg
      841 ttgttgcccg tgttgagtgc cgcacgggtt tggaccgttg gactaaacgg atcgtaggca
      901 ttcaactgct gggtcaccgt gttcgcgttg ctggcccagg cggtgatggt ataggtgtgc
      961 ggatcgtcgg ggctgttcga tcggcgagcg aaccagtaga cggcctggct gctgctggac
     1021 gccagcggcg gcagattgcg caggcgactt tcgcccggga gcaggaacca actcgcagtc
     1081 gggttcatgt cgaggtcgac gaagggcgct gtggccgtcg tcggcccggt gttcgtgatg
     1141 atcgcctcga ccgcccaaac gaccgggcta ctgataggat cgttgctatc gatggtggcg
     1201 aacggcgtgc tgtggatggc gatcgcgagg gaggtgcttg cctgcagcag cggcgccgca
     1261 ggcgggctga acggcggtgc ggagaccctc ccctcgcccc tcccgacacc cgtgagagaa
     1321 gccgcgctgc ccgttcgggc aggcacctga gagagaggcg ctgccagtgc accgaccggt
     1381 tgagccgccg tcagcaagcc tcccaggaca atcagcgccg acagcgcgat cgctagggaa
     1441 gcgaccaggg atcgcgccgc tgccagcgag tggctatacc gattgtgggg gcgtgacgag
     1501 tctgtcatgc aagacctccg tatctgtcgg ggaaaggcat ctgcttcagt caagaagagc
     1561 catcgtgtgt ggtgggggta gtgtgtgcta agttcagcgc cgtttatgga cggagcacga
     1621 gacatagaat accgcataaa cttgctgtca tgcgggcagg agcatattaa ggagtcatct
     1681 ctggaaaggc gaaccaagcg gtcttgccca ggggaccacc ggcccatgaa cgcacaccag
     1741 cttagcacag ttgagatggt ttgtaaatgt gactctttga caagtctata acagtttcgt
     1801 tgcaagttct ttgggcgcga ttccccggct cactcgggac ggctcaagtc gcaaaccggt
     1861 ctcaaaccat tagagcgagg cgcgtcgtct catccattac agcatgggtg gctgtgcctt
     1921 atgtcttggc caggatgcgg ataggcccac ggcaatcaga gtgtgacaaa atgtgacgag
     1981 gaacaggtca cccggcacct cacttttccc gctttgggcg ctacattgtt tgcatcttcg
     2041 agagacacac gggggatatt ccaatgtcac agcaatcgca cgacatcgtc gagccatcgg
     2101 cgcggtcgga ccggcgcaac ttgatggtcg ccggcgcggc gctgatcgcc atcgggctgc
     2161 tcgcgttcgc cgctcagttc atagagttcg tcgacgtcgg gctgctcttc ctgccgacgc
     2221 tgggcgtcat tttcctggtc tggggtatcg ccatccgccg cgtcgggccg atgatcccgg
     2281 gcggcatcct gaccgggatc ggcgtaggcg tcatcctgat ggagcagacc gcggccttgc
     2341 ccggcgaagc gtcagatgca ggcatcttcc tcatcacgtt cggcgcgggc tgggcgctca
     2401 ttacgctgct ctcggcgctg ttcacgccca agacacagtg gtggccgctg attcccggcg
     2461 cgatcatcgc ggcggttggc gccgcgctgt tcgcgggcga tgccggtctg cgcgtgctgg
     2521 aattcgccgg agacttctgg ccgctggtgc tcatcgccgc agggctgatc atcgtcctgc
     2581 gtgccgtgcg ctcggcgcga tagccacagt cacgaggcag ccaaagcttc ggaagtctct
     2641 acctcagaga gactcccgaa gtctgtatag gcagtttggt gtcttgcgtc ttcgcgctat
     2701 actggccgca attgaaaact tcggtttagc gggtcacgat agccaccgcc agccagccgt
     2761 cgcccagcgg atcgggtttg gcgacgacgg tgactgtgtc gccgacctgc ggagcgcgag
     2821 acgcgtcggg catgcgttgg gtcgccgcat cgatccggaa cgacgtcgtc tcaccctgct
     2881 ggccgacgag ctcgatccgg tcggcgctgg agttcgccac caggccgaga tagtgcatcc
     2941 gctgcggcgt ttccggcacg acggcgatct gcttcacggc gagctggccg ttcactttct
     3001 gcgcccacac cgcgaccttg tcgccgacct tgagctgttc aatgccggcg gccgggccgg
     3061 tgatcgtcat gaactgaagg ccctcgcgcg tttccaggct aagcccttcg gcgctgacgg
     3121 cctgcagcac cccgaccgct ccgacaggcg cttccaccgg cacctgcacc ggcgcttcga
     3181 cggcgctcga cggcgcgacg gcgtcaggct gggccacatt cactcctgtc gcctcgctcc
     3241 cattgcccag actgaagccg agcaatcctc ccactgccaa cgccgcaacc acgacaatcg
     3301 caaaactgta gagcttcatg gtgtccctcc taagatgatg ggttaggttg tagtacatct
     3361 tacactaaca gcggcgccat gtcacgcctc ccctgttccc aggcaaaata aagccccggc
     3421 gtgtctgccg gggccagatg tgggggcgat cagacctgcc aggtttgcga aacctggcgg
     3481 gtctcactca gagactatcc taaaatccgt ggttgacgca tccccatgcg tcaacagcgg
     3541 gcatagggat gcccgctcta cgggttctaa gacgcgctct cagggttgat acacgacggt
     3601 gaggatgacg aagacctctt cgccggcggt gttgctggcg atgacttcga gaacgtttac
     3661 ccctggcagc agcgcagccg gcaggctgaa tcggccttcg tcgttggcga agccgacggc
     3721 gtcgttgacg ctgacgagcg cccctggctg cgtctggccg acgaccagcc acggcgaggc
     3781 gctcacggtc tctccatcgc gcggcgtgtc gatggtcagc ggaaagatcg acgccggcgt
     3841 cggcgtggtc gagacggcga gcgtcggcgg aatctccggc acgatgatcg tcggcggcgg
     3901 cagtgtgggc tgcgcgggca gcggcgtggc cacgtcaacg atcaccggag tggatgtggg
     3961 gctggagatc ggagtctcag tcggctcggc gggcgacggc gttgttaccc ccgccagcgg
     4021 gtcgatacgc accggcggca cgagcgttac gagaatctgg ggcgtaacct ttggatcggg
     4081 caggcccgga atcaggcgcg acagcgtgcc gttcgagtag agcaggtaga caaccaaaat
     4141 gaccaacacg acgatcagca atgttctcat tgacacctcc ggcgccgcga gcgcggctgc
     4201 accgtgcgct tcggcgcgga gcgtattata tcgcgccgcg ccgtgttgac ccccggcgcg
     4261 attggcatac aatgggcgcc gccggaggga gccccatgca catcgtccat atcgacgccg
     4321 ccgatcgccg ccgcgtgaag cagtttctcg acttgccgtt tcgcctgtat cgcagtacgc
     4381 ctgagtgggt gccgccgctg tcgtccgatg cgcgccgcgt tttcgatcga aaacgccatg
     4441 cgttctacca gcactcggaa gcggcgttct tcctggcgat gaatggcgag cgcgccgccg
     4501 ggcgcatcgt cgccatcgac aatcggctgt acaacgagtt caacaaagag cgcacggcgt
     4561 tcttctacct cttcgagtgc gaggaggacc gggcggcgtc acagtcgctg ttcgacgcgg
     4621 cgttcgactg ggcgcgatcg cgcggcctgg acaaagtgat cggccccaag ggattcaccg
     4681 cgctcgacgg catggggctg ctggtcaagg gcttcgagca ccggccggcg tttggcatcc
     4741 cgtataacct gccctactac ccggcgctgg tcgaggcggc cgggttcgag cctgggggcg
     4801 acgtggtgtc gggttatctc agcacgaaga tgccgtttcc ggagaagatt catcaagtcg
     4861 ccgagatcgt gcagaaacgg cgcgggctgc gcgtggcgcg cttcaagtcg cggcgcgacc
     4921 tgctcgcgct gctgcccaga ctgcaggcga tgtacaacga ctcgttgggc ggcacgaccg
     4981 gcaacacgcc gctcaccgac gccgacatgc gagcgctggt caatcagctc tattggtttg
     5041 ccgatccgaa actgatcaag atcgtgatga agggcgagga accggtcggg ttctcgctgg
     5101 cctatcccga catctcggcg gcgatgcagc gcacccaggg acgattgctc cccttcggct
     5161 ggatcgacat gttgctcgag ctcagacgga cgaagtgggt gaatctcaac ggggccggga
     5221 tcgtcgagaa gtatcggggg ctgggcggca cggcgctgct gttcagcgag atgcacaaga
     5281 gcgtcgtcga gggcggcttc gagcatgccg atatcgtgca gatcggtgta gacaacgaca
     5341 agatgcagcg cgagctgcgc gacctgggga tcggtttcta caaaacgcac cgattgtatc
     5401 ggagggcgat atgatcttat ctcaatatct atagcgcgca acgcgccggg tggggaagag
     5461 ggagtctgct actgccacac gaccatcacc ggatcgcttt cggggctgag cgggccgatg
     5521 acattgccgg ccgcgtccac cttcacgacc gtcacggtcc acgcaaactc gcccttcgcg
     5581 ccgtcgggca cggggtagta acgccaggga tcgggcgtga ggtagctatc cacgatggcg
     5641 ccattcaggt acaggcgcat caaatagcgc tcgtcggcct gaatagacgc gtcgcgcagc
     5701 acccagcgga actcaaccgt ttcaccggaa tagtagcgag accctgccgg cggcttcagc
     5761 ggttcaacct cacgatagac gattgacaca ggcggcgccg tgctcgtctc cgttggcgct
     5821 tgcacgggaa ccgtcgacac agcccggcga ggggacgtcg ccgtcggcgt cggggtggcg
     5881 gtctccgtag ggagggacgt aggggtaggt gtataggtga acgtcgggat cggcgtgcgc
     5941 gtcggcgcaa gcgtctccgt cgctgtaggg atcggcccgg ctcccggctc gatgccgctc
     6001 aatatcagcg ccatcaccaa cccgccgatg ccgaacacga ctgcgaacgc gagcacgccg
     6061 tagacggcag aggacaacga gccgcgcacg ggcaccgccc gccaggcgcc cgggttgaca
     6121 cgattcatag cacgcacgag cacggcaaaa tcggcctcgc cccgcagctt gccgacgagc
     6181 gcggaggctt tagccgtctg cgtgtcgccg ggcagcgtca cctccagata tccgatttcg
     6241 ctgcagactc gagcgaactg ctcggctgac agccgattca gcgcgcgcac cgtgtctttg
     6301 ggcgtcggct ttgaggcgcc tgccatgaac gatccccccg ggaacttccc cgcgcgctca
     6361 ttttacctgt cttgtccctc agcgcaaccc gaaatgaaag acttcgggag ccacccatga
     6421 cttccgaagt ctgtgttcca gacggtgtga tgacagaatc gccgcgtcag acgaagaagc
     6481 ccacggcgag cgtgcccggc ccggcgtgcg tcgtgatcga cgcgcccacg ccgtagatcg
     6541 ggatgcgctc gatgcccagc gccggcttga gatcgccggc tagccgctgc gcctcgtcga
     6601 gatcgtcagc atgcatgacg cacaggtgcg caccggacga gcgagggcac tgctcgatcg
     6661 ccagatcctt cagtcgctcg aaggcgcggt gatgcgttcg cactttctcc tttgcctcga
     6721 cgtggccgtt cttgatctcg aggatcggtt tgatctgcag ggtgctgccg atcagggccg
     6781 atgccccgcc gatgcgcccg cccttctgca gatactccag cgtggcgacg aggaagtaaa
     6841 cgcgtccgcg cgggatcatg gcctgcaggc gactcacgat ctcgtcgacc tcaacgccgc
     6901 tctcggccca ctcgaccgcc agcatgacca gcgtcgccag gttgcccgcg accgtgcgcg
     6961 tgtcgatgac gcggatgtcg gcgtcgggaa acgaatcgcc tttcgccgtc agcgccgatt
     7021 gcaccgtggc actcacgtcg agagaaggat gaatggaaag gatcgtctgc gcttcagtca
     7081 gcagacgcgc gtacgcgtcg acgaaagcgc ccggcggcgg cgcggaggtt ttcggcaact
     7141 ggccggatga cttcagacgc cggatgaagt cgccgtaact cagctcgacg tcttctagaa
     7201 aggctgcctc tccgaaacgc acgatctgcg gaatgacctc gatcgaatgc gcggcgacga
     7261 actccggggg caggaccgcc gtgctatcag tgacgacgcg aattctggaa ggcacttccg
     7321 gcatgtccgt gatcgactga gactattcga ctttctcgac gacgatggtg cattcggcga
     7381 ccagcgcggc gatcgcgccg atggccgccc acaccggcgc cagcgcgatg ccgatcacgc
     7441 ccagcgtcag agggatttcg atcagcgtct tgtcgccctg ccggatgacg atgcggcgga
     7501 tgttgccctc gctgaccagt cgcttgatct cgctcagcaa ctgctccccc gtaaccttga
     7561 actcttcttt gcggtactgg cccttctctt caggcgctgc agatggctcg tccacgtcgt
     7621 ttacctccgt ttctttttgc ggcctcgcct acatgaccgt gatcgtcgcc gcgccaccct
     7681 cgatgcccag atcggtgcgg ttggggttgg tgccgtaatc gggcgactgg taggtattgc
     7741 tcccccactg gaagcgctgc ccgcgcacat tcagcgtcgc ggcgcccaca ttggactgca
     7801 cgcgcagcgc catgccgtca ggcacgcgca ctttcacgtc tgcggcgccg gccttgatcc
     7861 gcgccgacac ctgcccgctc gcagggagtg tcaagtcgac cgagctggcc cccacctcga
     7921 cgctgagagc ggtcaccttc acatcgcgca ggtcgatgcc ggccttgctc gcgccggtgt
     7981 gcacgtccag cgccaccggc acgtcgcgat aaatcgccat tgaccagcgg tatggggcgc
     8041 cccagtggcc gggccacatc cagaaccacc agtcgcgatc ttctcgtaag cgcacgtcga
     8101 ggcgatcgtc cacgcggcgc acgtctgaga tcacgccgcc gtccgacgtg ctgctgacca
     8161 acacggccgg atcatccgac gggccgatgc gcagctcgcc cgcgccgtga ttcacctcaa
     8221 tgcgcgcctg gcgcgcgccc tccaggggga ccgagttcga caccgcagcg gaggccccgc
     8281 gacgcacgag gctggcgagc agtagccagc cgcccagtcc aatgagcaac aagggaaaga
     8341 tcacgtccca gccgacgctc aggataccga aattttgcag cagaaagatc gcgcccagca
     8401 cgatgaggat tatcgcccaa aaaactgctc cacttctcca gcgcataaaa tgcctcctta
     8461 ccgttggaaa cccgattcgg cgcattcgag tttacaccta acgcgagcgc gtgtgaaccg
     8521 tcctccgacc gagttcgcta cgtcgcaggt ctgaactccg tcgtcatcca gcgcctggca
     8581 acccgtcgga gcgagcgggc caggctccca cgcccggcaa tttgccttga aacgcgctga
     8641 atcggcggcg tgcccgcttc gcgccgctgc gctacggggt gctttgcaag gggccgcctc
     8701 tgctgggttc tcgggcatac agttagagaa cacaaacgcg cggcgtactg ccgtaaagta
     8761 cgtcgcatag gccaggacat caagtagttt atcgcagcta tttgacttgt gtgttgcgca
     8821 gcagttcggc cgcctcgtcg tcgagcaggt ccatggccct gggatcggcc gtcttcggtc
     8881 gcctttccgc gagcatctgt ggcgtaacct tgtcggtcgg ctccacccgg ccgaagagcc
     8941 tggcggcggc gcgatgccag ccgtggaacg atcgcaccgg ctgcatgtac atcaggacgc
     9001 acgcaccgtg agccacgctt aatcctagct ctttgcccag ggcaagcgct tcaggcgact
     9061 cggcaccctg ctgcagccag acattgcgaa tgcccaaatc ggcggcgtcg cgcagcaccg
     9121 gcgtcacctt gttcgggggc aggctgacga tcaccccgtc cggcgtgcgc ggcagatcgc
     9181 gcagcgaagc gtagcacgtg tcaccttcga g
//
LOCUS       DBNHXB010000449         3463 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1651877, whole genome shotgun sequence.
ACCESSION   DBNHXB010000449 DBNHXB010000000
VERSION     DBNHXB010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1651877"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..640
                     /locus_tag="AC162G_17690"
     CDS             131..640
                     /locus_tag="AC162G_17690"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HIR5077692.1"
                     /translation="MTYRKIAMEHDKKIALVAHDNKKRDLVEWAQYNRDLLAHHQVYA
                     TGTTGEILEQELGFTITRLQSGPLGGDQQIGAKIVNGEIDFLIFFWDPLEPLPHDPDV
                     KALLRMAVVWNIPIACNRASADFMISSPLMDGEYDRLVPDYEGYRTRKIDWGEGARHG
                     AGDAAEGGA"
     gene            complement(683..1195)
                     /locus_tag="AC162G_17695"
     CDS             complement(683..1195)
                     /locus_tag="AC162G_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR5077693.1"
                     /translation="MLCLKLLVPSIVLAATLMWVLPALAEDPTPCLTSADKTCAATNN
                     QNLGAGKTRNGTLEAPWLVVSSDQMAARLGDVRNGLAAGNYAAGSYFFAEISCPSSTT
                     TYYASCSTTWFEMDNKGNIIKLSSQPGVPPKTGVDVPMPYLLAGGALLAVALITAGLV
                     LRRRAQRVVA"
     gene            1294..2001
                     /locus_tag="AC162G_17700"
     CDS             1294..2001
                     /locus_tag="AC162G_17700"
                     /inference="COORDINATES: protein motif:HMM:NF016119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HIR5077694.1"
                     /translation="MAPNDSAGGQKRLLAIGSWLIYIGIGLLVCFGGVFAYGYFETWA
                     ALQPQALVSNQENGPKSGPLTALPGISPARPARIEIPRIAVDRAVVTMGLVNRGEEPE
                     WDTDSLFATSGRPDLVGHLEGTANPGHPGNIVLAGHNYNRGVFNWSGVFYSLGTLQPG
                     DLVYLVDENDVRFTYRVDQVDQLPWPAPSADVALAHIKRLAPSPDEILTLVTCSGGTI
                     APFPARVYVTARRALDP"
     gene            complement(2047..2667)
                     /locus_tag="AC162G_17705"
     CDS             complement(2047..2667)
                     /locus_tag="AC162G_17705"
                     /inference="COORDINATES: protein motif:HMM:NF014845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V4R domain-containing protein"
                     /protein_id="HIR5077695.1"
                     /translation="MEDRPMPNANLRLLLVALQDVMGENGLKAVLKQGGLERYVDNFP
                     PDDTNRDAKFSEYGQIQQAVESFYGGPRGARAMMRRAGHATFQHTLKSRPAILGMVGV
                     ALKALPEGTRMKFILDRIARASTDEVNLPARVEEHDDHFMFVAEACTCVFRQRDPKLG
                     PCCFVTAGVLEAAIEWATGKRHTVKEIACLQNGDDACRYRVEKAAD"
     gene            complement(2684..>3463)
                     /locus_tag="AC162G_17710"
     CDS             complement(2684..>3463)
                     /locus_tag="AC162G_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077696.1"
                     /translation="IETLTLEGASRCVSDVAHARILHYNQSCDINHPAYRSLRAAAKA
                     HWMSTTPPGTDTTFYYPNKMGRIILIAIEEVMGRNGVNAVLNLAGLKHLINNYPPNNF
                     DRAFSFEEVSALLKALEDLYGPRGGRALAVRAGRAVFKFGLKDFGPILGIADLAFRIM
                     PLNMKMKVGFEVFAELFNKFTDQIVRLGEDEKAYYWIIERCPMCWGRKSEGPVGSLAT
                     GILQEGLYWLTGGKNFHVEETECVACNAHQGLFVIDKRPLD"
BASE COUNT          656 a         1072 c         1051 g          684 t
ORIGIN      
        1 tccgcgttcg gctcgtctat taggagagac ggcgaagacg gcgaaggagt tcccccggct
       61 gtgacgatcc gggatgcgac ggacatcggc gtcaaggcga tcgctgtgga gaaaggacgt
      121 aagaagacgt atgacttata gaaaaatcgc tatggaacat gataagaaga tcgctcttgt
      181 cgcacacgac aataagaaac gcgacctggt ggaatgggcg caatataacc gcgatctcct
      241 ggcgcaccat caagtgtatg caaccggcac caccggcgag atcctggaac aggaactggg
      301 cttcaccatc acaagactcc aaagcggccc tttgggcggg gatcagcaaa tcggcgccaa
      361 gatcgtcaat ggcgaaatcg attttctaat ctttttctgg gatccgctcg aacccctgcc
      421 gcatgatccc gacgtcaagg ccctcctgcg catggccgtg gtatggaata ttccgattgc
      481 ctgcaaccgg gcttcggctg atttcatgat ctcctcgccc ctcatggatg gcgagtatga
      541 tcgcctggtg cccgattatg agggatatcg cactcggaag atagactggg gcgaaggcgc
      601 acgccacggc gcaggggacg ccgccgaagg cggggcataa taaggatatg acgccagatt
      661 acatcccgga gtttttcgct cgttaagcga caacccgttg cgcccgccgg cgcagaacaa
      721 gcccggccgt gatgagcgcc acggccagca gagcgccgcc cgccaggagg taaggcatag
      781 gcacgtctac gcccgtcttt ggtgggacgc ccggctggct cgacagcttg ataatattgc
      841 ccttattgtc catctcaaac caggtcgttg agcagcttgc ataataggtg gttgtcgagc
      901 tgggacaact gatctcggcg aagaaatagc ttcccgccgc gtagttgccg gccgccagcc
      961 cgttccgcac gtcacccagg cgggccgcca tctgatcgct ggacaccacc agccacggcg
     1021 cttcgagcgt gccgttacga gtcttgcctg cgccgaggtt ctggttgttt gtggcggcgc
     1081 acgttttgtc ggcggacgtc aggcacggcg tagggtcctc ggccagggcg ggcaagaccc
     1141 acatcaacgt tgcagccagg actatagacg ggacgagcag cttgaggcac aacatctgct
     1201 ttctcctatg ctcgcttcat ttgacccggt ctggcatcga gcttattatc tcacaaaacg
     1261 ctacacccga ttctatcgcc gcacggacct gatatggctc cgaacgattc tgcaggcggc
     1321 cagaagcggc tcctggcaat cggctcctgg ctgatttaca tcggcattgg cctgctcgtc
     1381 tgctttggag gcgtcttcgc ctacggctat tttgagacgt gggcggcgtt gcagccgcaa
     1441 gccctggtct cgaatcaaga gaatgggccg aaatccggtc cactgacggc gctgcctggc
     1501 atctcgccgg cgcggcccgc gcgcatcgag attcccagaa tagccgtcga ccgcgccgtc
     1561 gtgacgatgg ggttagtgaa ccggggtgaa gagcccgagt gggatacgga cagcctgttc
     1621 gccacgtcgg gtcgacctga cctggttggg catctcgaag gaacggccaa cccgggccat
     1681 cccggtaaca ttgtgttagc cgggcacaac tacaaccgag gcgttttcaa ctggagcggt
     1741 gtgttctatt cgcttggaac tctgcagcca ggggatctgg tatatctcgt cgacgagaac
     1801 gacgtgcgat tcacttatcg ggtcgatcaa gtcgatcagc ttccgtggcc tgcgccatcc
     1861 gccgacgtcg ctctggcgca catcaaacgc ctggcgcctt cgcctgacga gatactcacg
     1921 ctcgtcacgt gcagcggagg cacgatcgcg ccgtttcccg cgagggtgta cgtcacggcg
     1981 cgcagggcgc tcgaccctta gaaatcatct caaaaaaagg tagtaacgac ttctgtcgtc
     2041 cggccatcag tctgccgcct tctcgacgcg gtaacggcac gcatcgtcgc cgttctgcag
     2101 gcacgcgatc tctttgacgg tgtggcgctt gccggtcgcc cactcgatcg ccgcctccag
     2161 cacgccggcg gtgacgaagc agcacggccc gagcttcggg tcgcgctgcc ggaacacgca
     2221 cgtacaggcc tcggcgacga acatgaaatg atcgtcgtgt tcctcgacgc gggcgggcag
     2281 gttgacttca tcggtcgagg ctctggcgat gcgatcaagg atgaatttca tgcgcgtgcc
     2341 ctcgggcagc gccttgagcg cgacgccgac catgcccagg atggccggcc gcgacttgag
     2401 cgtgtgctga aacgtggcgt ggccggcgcg gcgcatcatc gcccgcgcgc cgcgcgggcc
     2461 gccgtagaag ctctccacag cctgctggat ctgcccgtat tcgctgaact tcgcgtcgcg
     2521 gttcgtgtcg tccggtggga agttgtcgac gtagcgctcc agcccccctt gcttgagcac
     2581 cgctttcaag ccgttctcgc ccatcacgtc ctgaagcgcg accagcagca ggcgcaggtt
     2641 ggcgttgggc atgggtcgat cttccatggc ttcactcctc ttctcagtcc agcggtcgct
     2701 tgtcgatgac gaacagtccc tggtgcgcgt tgcaggcgac gcactcggtc tcctcgacgt
     2761 ggaaattctt gccgcccgtc agccagtaca aaccttcctg caggatgccc gtcgccagcg
     2821 acccgaccgg cccttccgac ttgcggcccc agcacatcgg gcagcgctcg atgatccagt
     2881 aataggcttt ttcgtcctcg cccaggcgca cgatctgatc ggtgaacttg ttgaacagct
     2941 cggcgaacac ctcgaatccg accttcatct tcatattcag cggcatgatg cggaacgcca
     3001 ggtcggcgat gccgaggatc gggccgaaat ccttgagacc gaacttgaac acggcgcgcc
     3061 cggcgcgcac ggccagcgcg cgcccgccgc gcggcccgta caggtcctcc aacgccttca
     3121 gcagcgccga gacttcctcg aacgagaaag cgcgatcgaa gttgttcggg ggatagttgt
     3181 tgatcaggtg cttgaggcca gccaggttga gcaccgcgtt cactccgttg cgccccatga
     3241 cctcttcgat cgcgatcagg atgatgcgcc ccattttgtt ggggtagtag aacgtggtat
     3301 ccgtcccggg cggggtagtc gacatccagt gcgccttcgc ggccgctcgc agcgagcggt
     3361 atgccgggtg atttatgtcg cagctctgat tatagtgcag gatgcgcgcg tgcgccacgt
     3421 ccgaaacaca tcgagacgcc ccctcaaggg tgagcgtctc gat
//
LOCUS       DBNHXB010000450        10219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2827132, whole genome shotgun sequence.
ACCESSION   DBNHXB010000450 DBNHXB010000000
VERSION     DBNHXB010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10219
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2827132"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="AC162G_17715"
     CDS             <1..497
                     /locus_tag="AC162G_17715"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5077697.1"
                     /translation="TRQAVVIDPGWNASSILAAVDSFQAVVKLILLTHAHFDHIGALA
                     DVVDATKAPVAMHPLDLPLLREDGGARAWGLPMRPAPDPDRSLEHGQVLEVGTLRFET
                     RFTPGHTPGHVVFYVESEKAVFDGDVLFQGAIGRTDFPGGDFDTLIASIRDQLLTLPD
                     DVVV"
     gene            complement(622..1788)
                     /locus_tag="AC162G_17720"
     CDS             complement(622..1788)
                     /locus_tag="AC162G_17720"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR5077698.1"
                     /translation="MSTYSTQQEAVGSLETLAELRTTLNRVMALESHTSLDNGGYEFR
                     GRLTVDSGEAFDHIYDRFTALGYTPVLFRHDGHEVVVAQRGVVKPKAANPRTNVILLI
                     LTIISTLFVGAVLYAPSPDLQQQLLVDPLSIFTGNASLLLNGLPYALTLLGILGIHEM
                     GHYFFARKHKAAVTLPYFIPMPLGLGTMGAVILLRSPIKNRRQLFDIGVAGPLAGLAV
                     AIPLLLVGLATSPVEFVSQPVPGYQEGNSILYLLLKYLVHGQILPSNGYDVILNAIAF
                     PAWFGLFITSLNLLPIGSLDGGHVMYAMYGRRQWRIARAAWMIMLIAGGAMLLLRTEL
                     GLNVWLLWAILVQMFGVMHPPPLDDITPLDARRRALGIFTMILFLLLIVPIPFS"
     gene            complement(1874..3763)
                     /locus_tag="AC162G_17725"
     CDS             complement(1874..3763)
                     /locus_tag="AC162G_17725"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019009890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077699.1"
                     /translation="MTIDTNHSLPAYPQQSLRQFRRLLAFVRPYRARLAIALGAIVVS
                     SLLGLAGPYMIQYLVNAIIPGGDPSVLNAVALILIGIFALQSVFYFVRGYLLAYVGER
                     VMVDMRLALYEHLQSLSQSFFTERRIGELVSRLTNDVATVRAVVTSDIATALSQTLTF
                     VGALALIIVISWQLTLFMLALIPPVVLLAILFGRRLRKLSSAVQDELASATTVLEESI
                     GGVRVVQSFTREQYEIGRFRKSIEATFALAMKRMRLSSTFGPLITFLAFSAIVAILWF
                     GGRQVLAGDLTTGQLVTFLILTMTLAGSIGQFSGLWAGVQEALGATQRLFEILDIRSD
                     VVELPDAATLMRAEGRITFQDVSFAYRPARADDTAPAVLDGVSIDVRPGETLALVGPS
                     GAGKSTLANLIPRFYDPTRGRILIDGVDIRTLTLKSLRGQIGVVPQETLLFGGAVREN
                     ILYGRLDASEAEMIAAAQAANAHDFITHLPQGYDAIVGERGVKLSGGQRQRIAIARAI
                     LKDPRILLLDEATSSLDSESEGLVQEALERLMKGRTSIVIAHRLSTVQNAHRIAVLDA
                     GRLVELGTHDELLARNGLYARLYRLQFRQDDSRPTPVSEPRPEPALARRRARLGILGQ
                     LAGQS"
     gene            complement(3885..6398)
                     /gene="lon"
                     /locus_tag="AC162G_17730"
     CDS             complement(3885..6398)
                     /gene="lon"
                     /locus_tag="AC162G_17730"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR5077700.1"
                     /translation="MSRQYSDSNQASFGFGDLYPPALSDLIQSPQAHARHGLIELPLL
                     PIRDTVLYPRLVSPLFVGRDRSVKAIEAAQVEGKNLIVAAQRDPEVEEPRPEELYAIG
                     CSALIIRTLRMPDGTTSVLTQGQYRAQIVEFVQTEPYFRVKARLIEESVEKSASTEAL
                     MRAVLALLEKVVNLNRSLPEDAYVYAMNIEEPGWLADLVASTIDIDLAERQQILEIFS
                     PAARLQALSIRLAKKLDVLELEDQIHTRVQREVDRSQREAYLREQMRAIQSELGEGDL
                     QAQEIAELRDKVSAKNMPDEVRAKASKELDRLQQMPPMAPEVGIIRTYLDWIIDLPWS
                     ETTTDDLDVAHAGEVLDQRHYGLPKAKERILEYIAVRRLAADKQRVRSPILCFVGPPG
                     TGKTSLGRSIAEALGRKFVRVSLGGIHDEAEIRGHRRTYIGALPGRIIQTMRRAETIN
                     PLFMLDEIDKLGADYRGDPSAALLEALDPEQNHAFSDHYLDVPYDLSKVMFVTTANIL
                     DTVPPALRDRMEVIEFPGYIEDEKLMIARKFIIPKQLEEHSLEPEQLHISDDAIRCIV
                     REYTYEAGVRNLEREIANICRKTARKMAESKPHRRRVASESVYRYLGPPRYVFGLAEE
                     KDQIGVATGVAWTESGGDVMPVEVTLMQGKGALLLTGQLGEIMQESAQAAVSYTRSRA
                     REFGIDRAAFEKTDIHIHVPEGAIHKDGPSAGITMATALISAFTRRPVRREVGMTGEI
                     TLRGRVLSIGGLREKTMAAHRAGLKTMIVPKKNKKDLVEIPKKVQRDLTFHFVESMDE
                     VLSVALRKSAKRKAPDAPLRRSRSTARPRASSKRPSAHR"
     gene            complement(6463..7923)
                     /locus_tag="AC162G_17735"
     CDS             complement(6463..7923)
                     /locus_tag="AC162G_17735"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR5077701.1"
                     /translation="MRTYEQALTYLNSFVNYERQRTAPYSAETLNLDRVRALLERLGN
                     PQHAYPTIHIAGTKGKGSTAAMIDSILHAAGYRTGLYTSPHLHTFRERMRVSGEPISR
                     ESLAALVDEVEPHVAGVEGVTWFEIVTALGFLHFARSAIDAGVIEVGLGGRFDATNVI
                     TPLVAVITSLSMDHMAWLGDTLEQIACEKAGIIKPGVPVVSAPQKPEALAVLERIAAE
                     RRAPLIVVGRDVRVERGAWGLDGQEFTVEASSDLMRGLSGPPFLAKTAFHIPLPGAHQ
                     VVNAAVAVTAVAVVSLSRSEFGSRAGSHARESAAWEPPPLAIWDRPSPEVSPIDTAAV
                     QTGLRNMHWPGRFEIARREPPLIFDGAHNADSAQKLASTLEECFPGQRWTLIFGSSSD
                     KDIAGMLDALLPLAERTIVTRARTSRAADVESIAQMVADRRHPVEIASSVHDAVQGAI
                     TLGEPAVVTGSLYIVAEAREAWLAHARLPMPDRDAG"
     gene            8126..9388
                     /locus_tag="AC162G_17740"
     CDS             8126..9388
                     /locus_tag="AC162G_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077702.1"
                     /translation="MSLRILAGHAVALVVLALLPACGPRELLSNVSFSPETISPNADG
                     DSDVTHISYDLGRNATVSIWLESSGGDRYTFRDARPRSAGEYDVQFSGVIDGRLLPDG
                     EYRWIVEASDEAGQRQAVDGRLAISGGESTAPQISGFSVDPPAFTPNRDGISDRTTIN
                     VSLNKPASLYVTLQNALCAGSTFPPEGQSDLDCTPYPMPENPSTGEARKPGEAGLHKF
                     DYDAGVDLGADPPPDGDYVVTARAEDTVGQVAIVTGTLTIVDGGVPRAEIVDGEVNFS
                     ARSLLIGETLYFTLTVENYGTVPIRTSGPPPGYVYDLDQNFNVPGFAEESGAWRVSID
                     FDTSLRDYPFRWAVGRPEDLAVEEIDGQKYYYLPSGARATITGGIRITDQPERNPLNF
                     WAGLIHEDVEITQINDRVDPHNITIDEP"
     gene            9591..>10219
                     /gene="phnD"
                     /locus_tag="AC162G_17745"
     CDS             9591..>10219
                     /gene="phnD"
                     /locus_tag="AC162G_17745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR5077703.1"
                     /translation="MPTRRSIYTLFSLMAVVSIILAACGGPALGSSDNPIVMSFVPSG
                     DTPEILASGEEIAKMISDRTGLTVKANVATSYAAVVEAMGAGQAHVGWLAPFSYVVAN
                     TKGYADVALSTVRFGSDHYGFQIIARADSGIASVADLSGKKLCWTDQLSTSGYVTPLG
                     YLADQGVDQATLDGGEFLGGHTAVVRGVYQGQCDAGATFVDARGSVEAD"
BASE COUNT         1785 a         3330 c         3236 g         1868 t
ORIGIN      
        1 ccacgcgcca ggccgtcgtc atcgatccgg gctggaacgc ttcctcgatt ctcgccgccg
       61 tcgattcctt ccaggctgtg gtcaagttga tcctgctcac gcacgcgcac ttcgatcaca
      121 tcggcgcgct cgccgacgtc gtcgatgcga cgaaagcgcc agtcgcgatg cacccgctcg
      181 acctgccgct gttgagagag gacggcggcg cgcgcgcgtg gggcctcccc atgcgccccg
      241 cccccgatcc cgaccgatct ctcgagcacg gccaggtgct cgaagtcggc acgctgcgct
      301 tcgagacgcg cttcacgccc ggccatacgc ccgggcacgt cgtgttctac gtcgagagtg
      361 aaaaagccgt cttcgatggg gacgtgctgt ttcaaggtgc catcggccgg acagatttcc
      421 cgggcggcga cttcgacacg ctcatcgcca gcattcggga ccagttgctc acgcttcctg
      481 acgacgtcgt ggtctagtcc ggccacggcc cgccgacgac gatcggcgct gagaggcgat
      541 tcaacccgtt tctatgacca acgacgaagg acgagcgacc gaagcgctcg tccctcgtcc
      601 ctggtcattc gtcctccgtc attaactgaa cggaatcggc acgatcagca acaagaacag
      661 aatcatcgtg aagatgccga gcgcgcggcg gcgcgcatcc agcggtgtga tgtcgtcgag
      721 cggcggcggg tgcatcacgc cgaacatctg caccaggatg gcccacagca gccatacgtt
      781 aagccccagc tcggtgcgca gcagcagcat cgcgccgccg gcgatcagca tgatcatcca
      841 cgccgcgcgg gcgatccgcc actgccggcg gccgtacatc gcgtacatca cgtgccctcc
      901 gtcgagcgac ccgatcggca acaggttgag cgacgtgatg aacagcccga accaggcggg
      961 gaaggcgatc gcgttcaata tgacatcgta gccgttgctc ggcaggatct ggccgtgcac
     1021 gaggtacttg aggagaaggt atagaatcga attgccttcc tggtagcccg gtaccggttg
     1081 gctgacgaac tcgaccggcg aggttgcaag tcccacgagc agaaggggaa tggccacggc
     1141 caggccggcg agtggcccgg ctacgccgat gtcgaacaac tggcggcgat tcttgatcgg
     1201 cgagcgcagc aggatcaccg cgcccattgt gccgagtccc agcggcatcg ggatgaagta
     1261 gggcagcgtg acggccgctt tatgcttgcg ggcaaagaag tagtgaccca tctcgtgaat
     1321 gcccagaatg cccaggagcg tcagagcata ggggagacca ttgaggagca gggaggcgtt
     1381 tccagtgaaa atcgagagcg ggtccacaag taactgctgc tgcaggtccg gcgacggcgc
     1441 gtagaggacg gcgccgacga acagtgtgct gatgatggtc agaatcagca gaatgacgtt
     1501 tgtgcgtggg ttcgccgcct tcggcttgac aacgccgcgc tgcgccacga cgacttcgtg
     1561 cccgtcgtgc cgaaagagca ccggcgtgta gcccagcgcc gtaaaccgat cgtagatgtg
     1621 atcgaacgcc tcgcctgagt ccaccgtcaa ccgcccccga aactcgtacc cgccgttgtc
     1681 caggctggtg tggctctcca gcgccatcac cctgttcagc gtagttctca gctcggccag
     1741 cgtttccagg ctgccaaccg cttcctgttg tgtcgaatag gtgctcaagt tcgcctcgta
     1801 accgacatga aattcgtctc aacctactat gaaactggat ctatcttagc actgtgcgcg
     1861 tcttcgcgtt gatctacgac tggcccgcga gctgccccag aattcccagc cgagcgcgcc
     1921 gacgggcgag cgccggctcc ggcctcggct cggacactgg ggtggggcgc gagtcgtcct
     1981 gcctgaactg cagccggtac agccgcgcgt acaggccatt gcgcgccagc agctcgtcgt
     2041 gcgtgcccag ttcgacgagc cggcccgcgt cgagcacggc gatgcggtgc gcgttctgca
     2101 ccgtgctcag ccggtgcgcg atgacgatgc tcgtccgtcc cttcatcagc cgttccagcg
     2161 cttcctgcac caatccctca ctctcgctgt ccagcgaaga ggtcgcttcg tcgagcagca
     2221 ggatgcgcgg atctttgagg atcgcgcgcg cgatggcgat gcgctgccgc tggccgccgc
     2281 tcaatttcac gccgcgctcg ccgacgatgg cgtcgtagcc ctggggcagg tgcgtgatga
     2341 aatcgtgcgc attggccgcc tgcgcggcgg cgatcatctc cgcctcgctc gcatcgagcc
     2401 ttccgtacag gatgttctct cgcaccgcac cgccgaacaa cagcgtctct tgcggcacca
     2461 cgccgatctg cccccgaagg ctcttgagcg tcagcgtgcg gatgtcaacg ccgtcgatca
     2521 ggatgcggcc ccgcgtcgga tcgtagaagc gcgggatcag gttggcaagc gtcgatttgc
     2581 ccgcgccgct tggcccgacg agcgccaggg tttcacctgg ccgcacgtcg atcgacacgc
     2641 cgtcgaggac ggctggcgcc gtgtcgtcgg cgcgcgctgg ccggtaggcg aatgagacgt
     2701 cctggaacgt gatgcgacct tcggcgcgca tcaatgtggc tgcatctggc agctcgacga
     2761 cgtcggaccg gatgtcgaga atctcgaaca gccgctgcgt cgcgccgagc gcctcctgta
     2821 cacccgccca aagcccgctg aactgcccga tcgagccggc gagcgtcatc gtcaggatca
     2881 ggaacgtgac gagctgcccg gtcgtcaggt cgccggccaa cacctgccgg ccgccgaacc
     2941 acaggatggc gacgattgcg gagaatgcca gaaaggtgat cagcgggccg aaagtcgacg
     3001 agaggcgcat gcgcttcatc gccagcgcga aagtcgcttc gatactcttt cggaagcggc
     3061 cgatctcgta ctgctcgcgc gtgaacgatt ggacgacgcg cacgccgccg atcgattcct
     3121 cgagcactgt cgtcgcgctg gccagctcgt cctgtacggc ggacgagagc ttgcgcagcc
     3181 gccgcccgaa caggatcgcc agcagaacga ccggcgggat cagcgccagc atgaatagcg
     3241 tcagttgcca actgatcacg atgatcagcg ccagcgcgcc gacgaacgtc agcgtctgcg
     3301 ataaggcagt ggcgatgtcg ctcgtgacca cggcgcgcac ggtggcgacg tcgttggtca
     3361 atcgcgacac cagctcgcca atgcgccgtt cggtgaagaa gctctgtgac aggctctgca
     3421 aatgctcgta caacgccaac cgcatgtcga ccatcacccg ctcgccgacg taggccagca
     3481 gataaccgcg cacgaagtag aacacactct gcagggcgaa gatcccgatc agaataagcg
     3541 cgaccgcatt cagaacgctc ggatcgccgc cgggaatgat cgcgttgacg aggtactgga
     3601 tcatgtacgg cccggccagc ccgagcaggc tgctcacgac gatcgcgccc aacgcgatcg
     3661 ccaggcgcgc gcggtagggc cgcacgaaag cgagcaggcg gcgaaattgc cgcagagatt
     3721 gttgcggata ggcgggcaga ctgtggtttg tatcgatcgt cacgtgcaca gtatatcgtt
     3781 gccaacgccc accgtcaagg cgcgaagccc cctggcctct ccgagaaacc agggggctgc
     3841 ccaatttgcg cacgatgccg cttctcggtg aagcggggcg ggagttaccg atgcgcggac
     3901 ggtcgcttcg aagacgcgcg cggtcgcgcc gtggagcggc tgcggcgaag cggcgcgtcg
     3961 ggcgctttcc gtttcgccga tttccgcagc gccacggaca acacttcgtc catcgattcg
     4021 acgaagtgaa aggtcaggtc gcgctggacc ttcttgggga tttcgacgag gtccttcttg
     4081 ttcttcttcg gaacgatcat cgtcttcagg ccggcgcggt gcgcggccat cgtcttctcg
     4141 cgcagcccgc cgatggatag gacgcggccg cgcagcgtga tctcgccggt catgcccacc
     4201 tcgcgccgca ccgggcggcg cgtgaaggcg gagatcagcg cggtggccat cgtaatgcct
     4261 gccgaaggcc catctttgtg gatcgcgcct tccggcacgt ggatgtgaat gtcggtcttc
     4321 tcgaacgccg ctcgatcgat gccgaactcc ctggcgcgcg agcgggtgta cgacacggcg
     4381 gcctgcgccg attcctgcat gatctcgccg agctggccgg tgagcagcag cgcgcccttg
     4441 ccctgcatca gcgtcacttc gaccggcatc acgtcgccgc cggactccgt ccacgccacg
     4501 cccgtcgcca caccgatctg gtctttttcc tcggccaggc cgaagacgta gcgcggcggc
     4561 cccaggtaac ggtacacgga ttcggacgcc acgcggcggc ggtgcggttt cgactcggcc
     4621 atcttccgcg ccgtcttgcg gcaaatgttg gcgatctcgc gctccaggtt tcgcaccccg
     4681 gcttcgtagg tgtattcgcg cacgatacag cgaatcgcgt cgtcggagat gtggagctgc
     4741 tccggctcca ggctgtgctc ttccaactgc ttcggaatga tgaatttccg ggcaatcatc
     4801 agcttctcgt cctcgatgta gcccgggaac tcgatgacct ccatccggtc gcgtagcgca
     4861 ggcgggacgg tgtccaggat gttggctgtc gtgacgaaca tcaccttcga cagatcgtag
     4921 ggcacgtcga gatagtgatc gctgaaagcg tggttctgct ccgggtcgag cgcttccagc
     4981 agcgccgcgg acgggtcgcc gcgatagtcg gcgccgagct tgtcgatctc gtcgagcatg
     5041 aacaagggat tgatcgtttc ggcccggcgc atcgtctgga tgatgcgccc cggcagcgcg
     5101 ccgatgtacg tgcggcggtg gcctcgtatc tccgcttcgt cgtggatgcc gcccaggctc
     5161 acgcgcacga acttgcggcc gagcgcctcg gcgatcgatc tgccgagcga ggtctttccg
     5221 gtgccgggtg gtccaacgaa acacagaatc gggctgcgca cacgctgctt gtcggcggcc
     5281 aggcggcgca cggcgatgta ctccaggatg cgttctttcg ctttcggcag gccatagtgg
     5341 cgctggtcga gcacttcacc cgcgtgcgcc acgtccaggt cgtccgtcgt cgtctccgac
     5401 cacggcaggt cgatgatcca gtcgaggtag gtgcggatga tgccgacctc gggcgccatc
     5461 ggcggcattt gctgaaggcg atccagctcc ttgctcgctt tggcgcgcac ctcgtcgggc
     5521 atgttcttcg cgctgacttt gtcgcgcagc tccgcgatct cctgagcctg caggtcgcct
     5581 tcgcctagct ccgactgaat cgctcgcatt tgttcacgca ggtatgcctc gcgctgcgat
     5641 cgatccacct ctcgctgcac gcgcgtgtgg atctgatcct ctagctccag cacgtcgagc
     5701 ttcttggcga ggcggatcga cagggcctgc aggcgcgcgg ccgggctgaa gatctccagg
     5761 atttgctggc gctcggcgag gtcgatgtcg atcgtcgacg cgaccaggtc ggccagccag
     5821 cccggctctt cgatgttcat cgcgtacacg taggcgtctt caggcaggct gcggttgagg
     5881 ttgacgacct tctcgagcaa ggccagcacg gcgcgcatga gcgcctcggt cgaggccgac
     5941 ttttcaacgg attcttcgat cagccgcgcc ttcacgcgga aatacggctc cgtctgcacg
     6001 aactcgacaa tctgcgcgcg atactgccct tgcgtcaata cgctggtcgt gccatcgggc
     6061 atgcgcagcg tgcggatgat cagagcgctg cagccgatcg catatagctc ctccgggcgc
     6121 ggctcctcta cttccgggtc ccgctgcgcg gcgacgatca agttcttgcc ttccacctgc
     6181 gcggcctcga tcgccttcac cgatcgatcg cgcccgacga acagcggcga caccaggcgc
     6241 ggataaagca ccgtgtcgcg aatgggcagc aaaggcagct cgatcagccc gtgacgagcg
     6301 tgcgcctggg gcgactgaat cagatcgctc aaggcaggcg ggtacaaatc gccgaatccg
     6361 aatgaagcct ggttgctatc gctgtactgt ctcgacacaa acactctccg cgttgctatg
     6421 gagcctatgc gattctaact caaaagcttc agggatgcca actcacccgg cgtcccgatc
     6481 gggcatcggc agccgggcgt gcgcgagcca tgcttcacgc gcctcggcga cgatgtacaa
     6541 tgagccggtg acgacagccg gctctccgag cgtgatggct ccctgcacgg cgtcgtgcac
     6601 gctcgacgcg atctcgactg ggtgccggcg gtcggcgacc atttgcgcga tcgactcgac
     6661 atcggcggcg cgcgacgttc tcgcgcgtgt cacgatggtc cgttcggcca gcggcagcag
     6721 tgcatcgagc atgcctgcga tgtccttgtc ggacgacgag ccgaagatca gcgtccatcg
     6781 ctggcctgga aaacactctt ctagcgtcga ggccagcttt tgcgcagagt cggcgttgtg
     6841 cgcgccgtcg aagatcaggg gcggctcgcg ccgcgcgatc tcgaaacgtc ccggccaatg
     6901 catgttcctc agccctgtct ggacagccgc cgtgtcaatc ggtgacacct ccggactcgg
     6961 tcgatcccat atggctagcg gaggcggctc ccatgccgcc gattcgcggg catgggagcc
     7021 cgctctgctt ccgaattcgg atctactgag actcaccacc gcgacggcgg taaccgccac
     7081 tgccgcgttg acgacctggt gcgcgcccgg caatggaatg tgaaacgcag tcttcgccag
     7141 aaacggcggg ccggaaaggc cacgcatcaa gtcgcttgac gcttccacgg tgaactcttg
     7201 accgtcaagt ccccacgctc cgcgctctac cctgacatcc ctcccgacaa cgatcagcgg
     7261 cgcgcgtctc tccgccgcga ttcgttccag cacggccagt gcctccggct tctgcggcgc
     7321 gctgacgacc ggcacgcccg gcttgatgat gccagccttc tcgcatgcga tctgctcgag
     7381 cgtgtcgccc agccaagcca tgtggtccat gcttagagac gtgatgactg ctacgagcgg
     7441 agtgatgacg ttcgtcgcgt cgaagcggcc gcccaacccg acttcgatca cgccggcgtc
     7501 gatcgccgac cgggcgaagt gcaggaagcc gagcgcggtg acgatctcga accaggtgac
     7561 gccctcgacg ccagcgacgt gcggctcgac ctcgtccacc agtgcggcga gcgactcgcg
     7621 cgaaatcggc tcaccgctca cgcgcatccg ctcgcggaac gtgtgcaagt gcggcgatgt
     7681 gtacagcccc gttcggtatc ccgccgcgtg caggatcgaa tctatcatcg cggcggtaga
     7741 gcctttgcct ttcgtgccgg cgatgtggat cgtcgggtag gcatgctgcg ggttacccag
     7801 ccgctcgagc agcgcgcgca cgcggtcgag gttgagcgtc tcggcggagt acggcgctgt
     7861 gcgctgccgt tcgtagttga cgaatgagtt caaataggtg agcgcctgct cgtacgtgcg
     7921 cacgtgtctc tcgcgggttg catggtgccc gcattgtatg cgatttgcgt gcaactgcaa
     7981 tcccccggca gacctggcag gtctcgaaga cctgccaggt ctacatgact tcgcacccag
     8041 gacgcgcgtg cttgacactc tcccttacgt cgattagact gcgcgccggg aagccccaca
     8101 ttcgtcaggc cgagaggatc cattgatgtc cttgcgaatt ctggcgggcc acgccgtcgc
     8161 tctggtcgtg ctggcactgc tgccggcgtg cggcccgcgc gagctgcttt cgaacgtgtc
     8221 cttctcgccg gagaccatct cgccgaacgc cgacggcgat tcggacgtga cccatatcag
     8281 ctacgatctc ggtcgcaatg ccaccgtatc gatctggctc gagtcgagcg gcggcgaccg
     8341 ctacactttc cgggatgccc ggccccgttc ggcgggcgaa tatgatgtgc agttcagcgg
     8401 cgtgatcgac ggccgcttgc tgccggatgg cgaatatcgc tggatcgtcg aagcgtccga
     8461 cgaggcagga cagaggcaag ccgtcgacgg ccggttggcg atctcgggcg gcgaatcgac
     8521 tgcgccgcag atcagtggct ttagcgttga cccgcctgca ttcacaccga atcgcgacgg
     8581 catttcggat cggacgacga tcaacgtcag cctcaacaag cccgcatcgt tatacgtcac
     8641 gctgcagaac gctctttgcg ccggctcgac gttcccgccc gagggtcaat ccgacctgga
     8701 ctgcacaccg tacccgatgc ccgagaaccc ctcgacaggc gaagcgcgca agcccggcga
     8761 agccggcctt cacaagttcg actacgacgc cggcgtcgat ctgggcgccg atcccccgcc
     8821 ggatggcgac tacgtcgtca cggcgcgcgc cgaggatacc gtcgggcagg tggcgatcgt
     8881 gaccggcacg ctgaccatcg tcgacggggg cgtgccgcgc gccgagatcg tcgacggcga
     8941 ggtgaatttc tcggcgcgct cgctcctgat cggagaaacc ctctacttca cgctgacggt
     9001 cgagaactac ggcacagtcc ccattcgcac cagcggcccg ccgccgggct acgtctacga
     9061 tctcgatcag aacttcaacg tgccgggatt cgccgaggag tcgggcgcgt ggcgtgtgag
     9121 catcgacttc gataccagcc tgcgcgacta ccccttccgc tgggccgtcg gccggccgga
     9181 agacctcgcc gtcgaggaga tcgacggcca gaagtattac tatcttccgt ccggcgcccg
     9241 tgccacgatc acgggcggca ttcgcatcac cgaccaaccg gagcgcaatc cgctgaactt
     9301 ctgggcaggg ttgatccacg aagacgtgga gatcacccag atcaatgaca gggtcgatcc
     9361 gcacaacatc acgatcgatg agccgtaaca ttgggtcatc ttatcctcgt ccggcttgtc
     9421 atccggcaag acttgttgta ccatgtgtct tgctctccaa ctgcgaaccc attctttgcg
     9481 ccgtgatagg atgagaggag gtgatgcttc tcgacgcagg ttgttacccc gcaacgggta
     9541 gttacgtctc atctttggca cgattccatt caaaaggaga agagaagaag atgcctaccc
     9601 gacgttcgat ctacacgttg ttcagtttga tggccgtcgt ttcgatcata ctggcggcat
     9661 gtggcggacc ggcgctggga tcctccgaca acccgatcgt catgtccttc gtgccgtcgg
     9721 gcgatacgcc ggagatcctc gccagcggcg aagagatcgc caagatgatc agtgacagga
     9781 ccggcctcac cgtcaaggcc aacgtcgcca cgtcgtacgc ggcggtggtg gaggcgatgg
     9841 gcgcaggcca ggcgcacgtg ggctggctgg cgccattctc gtacgtcgtg gcgaatacga
     9901 agggctacgc cgacgtggcg ttgtcgacgg tgcgcttcgg cagcgaccac tacggtttcc
     9961 agatcatcgc ccgcgctgac tccggcattg cgtcggtcgc ggacttgagc ggcaagaagc
    10021 tgtgctggac cgatcagttg tccacctccg gctacgtgac gccgctgggt tacttggcag
    10081 accagggcgt cgatcaggcg acgctggatg gcggtgaatt cctgggtggc cacaccgccg
    10141 tcgtgcgcgg cgtgtatcag gggcagtgtg atgcaggcgc gacgttcgtc gatgcgcgcg
    10201 gcagcgtgga ggcagactt
//
LOCUS       DBNHXB010000451         4844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4169741, whole genome shotgun sequence.
ACCESSION   DBNHXB010000451 DBNHXB010000000
VERSION     DBNHXB010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4169741"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..4303)
                     /gene="rpoC"
                     /locus_tag="AC162G_17750"
     CDS             complement(50..4303)
                     /gene="rpoC"
                     /locus_tag="AC162G_17750"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR5077704.1"
                     /translation="MEAKGFRALRVSLASPDRILSWSYGEVTKPETINYRRLRPEKDG
                     LFCEAIFGPTKDWQCYCGKYKNVRYRGITCDKCGVEVTRAAVRRERMGHIELAAPVAH
                     VWYTRRVPSYLGVLLDVSRRNLDRVLYFAQYVVTHVDEEARQKALRRLDEETDRERKR
                     VEATARESIRAIKTRVEAEVDKRKEQIEQRAGRLDEKLNEATEAVMKEAQTIQARIEE
                     RMGKDIRTPIVFAPTKTLIADRGEAIGREHLTKLNKAVKAKLAELEARNSAEREKALS
                     KIEADIDYWKAAAQEEQAEIETQLAGDLAKLDDRGGGEREELQSLGQGQFLTEARYRD
                     LKSKWGQVFKADMGAEAFLDILKRMDLDQMSKELWREVKTSKSKQKRKKAIKRLRVVE
                     SLRKSANRPEWMILTYLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLL
                     ELGAPDVIVRNEKRMLQEAVDSLIDNSQRGKALSRRGRRELKSLSDMLKGKKGRFRRN
                     LLGKRVDYSGRSVIVIGPKLKLHQCGLPKTMALELYRPFVISKLVEYNFASNVKGAKR
                     IIERERPEVWEVLEEVIKQRPVLLNRAPTLHRLGIQAFEPVLVEGKAIQIHPLVCPAF
                     NADFDGDQMAVHVPLSDMAVTEARDLMLSTKNLLKPASGEPIVGPAKDMVLGVYYLTM
                     ARTGRQGEGRVFASLEEVELAHDLGLVDLHAQIKLQTETVYTSGSERHADGQAHTQLI
                     DTTVGRALFNMILPSPLRYVNEVLDKGKLNDLVARCYLWLGLNATTNLVDAIKNIGFE
                     YATHSGTTIAVSDFVVPDEKQHILGEIEAQVNEVDRQYRRGLLTEKEQYTRTIELWQQ
                     ATGRVADAVKRALDPEANLSVMALSGATKGGFGPISQLAGMRGLMADPAGNIIALPIK
                     SNFREGLTALEYFISTHGARKGLADTALRTADAGYLTRRLVDVAQDQIVNAHDCGTKS
                     GIWIRRSDNVGGQSIHVRIIGRILAGPVTDPQTGELIGSAGDMIDESMAHRIDNSGVP
                     EVYARSPMTCELRHGLCQLCYGRDLGRGTLVEIGAAVGIVAAQSIGEPGTQLTLRTFH
                     TGGVAAEGDITHGLPRVEELFEARKKPKGEAVMSDIDGDVQIVRGEDSVRKIKIVFTQ
                     NARDEYDVPGNWSLKVEDGQTISDGDLIASRGDDKIAAKRAGKVQVRKNKPLAVVYEV
                     RDEREYEVPPTARLLVEDGQAVVAGQQLSEGALNPHRILRILGRDATQLYILSDIQQV
                     YRSQGQNINDKHFEVIVRKMLSRVQIVSSGDSDMLPGELVDRLQLQDINESLLAASKR
                     PATAAPVLLGVTKAALTTDSFLSASSFQHTIKVLAGAAIEGKTDELFGLKENVIIGKL
                     IPAGTGFKQPAMIAEPPDVSEDVTSGSEPLEATLPAAEA"
     gene            complement(4636..>4844)
                     /gene="prfB"
                     /locus_tag="AC162G_17755"
     CDS             complement(4636..>4844)
                     /gene="prfB"
                     /locus_tag="AC162G_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_102136549.1"
                     /note="recognizes the termination signals UGA and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF2; in some organisms
                     control of PrfB protein levels is maintained through a +1
                     ribosomal frameshifting mechanism; this protein is similar
                     to release factor 1; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR5077705.1"
                     /translation="NQRKALAKLKGEHVKAEWGSQIRSYVLHPYQMVKDHRTGHEVGN
                     AQVVLDGRLDEFMGSYLHLAVAES"
BASE COUNT          792 a         1640 c         1445 g          967 t
ORIGIN      
        1 gcggcagcga tcaagctgtg atcgctgccg ccggttcgct acggtctgac tacgcctccg
       61 ctgccggaag cgtcgcctca aggggctccg atcctgatgt gacgtcttca gacacgtcgg
      121 gcggctcggc tatcatcgct ggctgcttga agccggtgcc ggctgggatc agcttgccaa
      181 tgatgacgtt ctctttcagg ccgaagagtt cgtccgtctt gccctcgatg gccgcgcccg
      241 ccagcacttt gatggtgtgc tggaacgatg atgcgctcag gaacgagtcc gtcgtgaggg
      301 ctgccttcgt cacacccagc agcaccggcg ccgccgtcgc aggtcgcttg ctcgccgcca
      361 gcagcgactc gttgatatcc tgcaactgga gccgatccac aagctcaccc ggcagcatgt
      421 cggagtcccc gctcgacacg atctgcacgc ggctgagcat cttgcggacg atcacctcga
      481 agtgcttatc gttgatgttc tggccctggg agcggtagac ctgctggatg tcgctcagaa
      541 tgtacaactg ggtcgcgtcg cgtcccagga tgcgcagaat ccggtgcggg ttcagggcgc
      601 cttcactcaa ctgctggccg gccacgacgg cctgcccatc ctcaacgagc aggcgtgccg
      661 tcggcggcac ctcgtactcc cgctcgtcgc gcacctcgta gaccacggcg agcggcttgt
      721 ttttgcgcac ctgcaccttg ccggcgcgct tggcggcgat cttgtcgtcg ccgcgactgg
      781 caatcaggtc gccatcgctg atcgtctggc cgtcctcgac tttcagcgac cagttaccgg
      841 gcacatcgta ctcgtcgcgc gcgttctgag tgaagacgat cttgatcttg cgcacactgt
      901 cttcgccgcg cacgatttgg acgtcgccgt cgatgtccga catgacggcc tcgcccttcg
      961 gcttcttgcg cgcctcgaac agctcttcga cgcgcggcag gccatgcgtg atgtcaccct
     1021 cggccgcgac accgcccgtg tgaaaggtgc gcagcgtcaa ctgtgtgccc ggctcgccga
     1081 tcgactgagc ggcgacgatc ccgaccgccg cgccgatctc gaccagtgtg ccgcgaccca
     1141 aatcgcgccc gtagcacaac tggcacaggc cgtggcgcag ctcgcacgtc atcggagagc
     1201 gcgcgtagac ctcgggcacg ccggaattgt cgatacggtg tgccatgctc tcgtcgatca
     1261 tgtctccggc gctgccgatc aactcgcccg tctggggatc cgtcaccggg ccagccagga
     1321 tacggccgat aatccggaca tgtatgctct ggccgcccac gttgtcgctg cggcgaatcc
     1381 agatgccgct cttcgtgccg cagtcgtgcg cgttaacgat ctgatcctgc gcgacgtcga
     1441 ccagacgccg cgtgaggtag cctgcgtcgg cggtgcgcag cgcggtatcg gccagaccct
     1501 tgcgcgcgcc gtgcgtcgag atgaagtact cgagagcggt caacccttcg cggaagttgc
     1561 tcttgatcgg gagcgcgatg atgttgccgg ccgggtccgc catcagaccg cgcataccgg
     1621 ccaactgcga gatagggccg aagccgcctt tcgtcgcgcc tgacaaggcc atcacgctca
     1681 ggttcgcttc gggatcgagc gcgcgcttga cggcatcggc cacgcggccg gtcgcctgct
     1741 gccacagctc gatcgtgcgc gtatattgct ccttctccgt cagcagaccg cgacgatact
     1801 gccgatccac ttcgttgacc tgggcctcga tttcgccgag aatgtgctgc ttctcgtccg
     1861 gcacgacgaa gtccgagacg gcgatcgtcg ttccggagtg cgtggcatac tcgaaaccga
     1921 tgttcttgat cgcatcgacc aggttcgtcg tcgcattcag ccccagccac aggtaacagc
     1981 gtgcgacgag atcgttgagc ttacctttgt ccaggacctc gttcacgtag cgcagcggcg
     2041 acggaaggat catgttgaac agggcgcggc cgacggtcgt gtcgatcagt tgcgtgtgcg
     2101 cctggccatc cgcatgccgc tcactgccgg aggtgtaaac cgtctcggtt tggagcttga
     2161 tctgggcatg caggtcaacc aggcccaggt cgtgggccag ctcgacctct tcgaggctgg
     2221 cgaaaacgcg cccctcgccc tggcggcccg ttctggccat ggtcagatag taaacgccca
     2281 gcaccatgtc cttcgccggc ccgacgatcg gctcgccgga cgccggcttc aggaggttct
     2341 ttgtcgagag catcaagtcg cgcgcttccg tcaccgccat gtcggagagc ggcacgtgca
     2401 cggccatctg gtcgccgtcg aagtcggcgt tgaaggccgg gcacaccagc gggtgaatct
     2461 gaatcgcctt gccctcgacg agcaccggct cgaaggcctg gatgcccagg cggtgaagcg
     2521 tcggcgcgcg attgagcaac accggccgct gcttgatcac ctcttcgagc acttcccaca
     2581 cttcaggacg ttctcgctcg atgatgcgct tggcgccctt gacgttgctg gcgaagttat
     2641 actcgaccag cttgctgatg acgaaggggc ggtacagctc cagcgccatc gtcttcggca
     2701 ggccgcactg gtgcagcttg agcttcggac cgatgacgat caccgagcgc ccggagtaat
     2761 ccacgcgctt gccgagcagg ttgcggcgga agcggccctt cttgcccttg agcatgtcgc
     2821 tcaaggactt caactcgcgc cgcccgcgcc gcgagagcgc cttgccgcgc tggctattgt
     2881 cgatgagcga gtcgacggct tcctgcagca tgcgcttctc gttgcgcacg atcacgtcgg
     2941 gcgcgcccag ctccagcagt cgcttcagcc ggttattgcg gttgatgacg cgccggtaca
     3001 aatcgttcag atcggacgtg gcgaaacggc cgccgtcgag ctgcaccata ggccgcaagt
     3061 cgggcgggat gaccggcagg taggtcagga tcatccactc cggccggttg gcgctcttgc
     3121 gcagactctc cacgacgcgc agccgcttga tcgccttctt gcgcttctgc ttgctcttgc
     3181 tggtcttgac ctcgcgccaa agctccttgg acatctggtc gaggtccatg cgcttcagga
     3241 tgtcgaggaa agcctccgcg cccatgtcgg ccttgaagac ctgtccccac ttgctcttga
     3301 gatcccggta gcgcgcttcg gtcagaaact ggccctggcc cagagactgg agctcctcgc
     3361 gttcgccgcc cccgcggtcg tcgagcttcg ccaggtcccc ggccagttgg gtctcgatct
     3421 cggcctgctc ctcctgagcc gctgccttcc agtaatcgat gtcggcttcg atcttggaga
     3481 gcgccttctc gcgctcagcg ctgttcctgg cttccagctc ggcgagcttg gccttgacgg
     3541 ccttgttgag cttcgtcaaa tgctcgcgcc cgatcgcctc gccccggtcg gcgatcagcg
     3601 tcttcgtcgg ggcgaagacg atgggcgtgc ggatatcttt gcccatgcgc tcctcgatgc
     3661 gcgcctggat cgtctgggcc tccttcatga cggcctcggt cgcctcgttc agcttctcgt
     3721 cgagccggcc ggcgcgctgc tcgatctgct ccttgcgctt gtcgacctcg gcttccacac
     3781 gggtcttgat cgcgcgaatg ctttcacgcg ccgtcgcctc gacccgcttg cgctcgcgat
     3841 ccgtctcttc gtccaggcgc ctgagcgcct tctggcgcgc ttcctcgtcg acgtgcgtca
     3901 cgacgtactg cgcaaagtac agcacgcgat ccagattgcg ccggctgacg tcgagaagca
     3961 cgccgaggta ggacgggacg cggcgcgtgt accacacgtg cgcgacgggc gcggccagct
     4021 cgatgtgccc catgcgctcg cgccgcacgg ccgcacgcgt cacctcgacg ccgcacttgt
     4081 cgcacgtgat accgcgatac cggacgttct tgtacttgcc gcaatagcac tgccagtctt
     4141 tggtcgggcc aaagatcgcc tcacagaaca agccgtcctt ttcaggccgc aggcggcgat
     4201 agttgatcgt ctccggcttg gtcacctcgc cgtacgacca cgacaagatg cgatccggcg
     4261 aagccaaact gactcgcagc gcacgaaaac ccttcgcttc cactcacgcc tcctctcgag
     4321 aatgcgacct atccaaccat acctttacca acgaccaccc cgagccactc actcatcacg
     4381 taatggcaaa tccactccca ggcaagccaa aagaagcgct gaatttacgc cgttcagcgc
     4441 tcctggcaga tggagtgata aacctttaag cgagaatacg cacccgctcc cgcccgctgc
     4501 acgtgacctt ccggcagcca cagggacggc ggatgaaaat acccactgct ccgccacaca
     4561 gcctgtcgac aggctgagca atgtaattat atctgcatcc tgggaaatgt caagcaaaaa
     4621 atggccgctc ggccgctagc tttccgccac cgccaggtgc agatacgacc ccatgaactc
     4681 gtcgaggcgg ccgtcgagga ccacctgcgc gttgcccact tcgtggccgg tgcgatgatc
     4741 cttgaccatc tgatagggat gtagaacata tgagcgaatc tggctgcccc actctgcctt
     4801 gacgtgctcg cccttcagct tggccagcgc cttgcgctga ttct
//
LOCUS       DBNHXB010000452        10566 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2996220, whole genome shotgun sequence.
ACCESSION   DBNHXB010000452 DBNHXB010000000
VERSION     DBNHXB010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10566
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2996220"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="AC162G_17760"
     CDS             complement(<1..290)
                     /locus_tag="AC162G_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077706.1"
                     /translation="MKRLKSVLTPPRIVPAPAPLAPRWPLYAGVALAIGLASLLYLNT
                     LSLPFFQDDVIHIRWLSTHSLVDPFLTAENLPTYRPLGESLMKLWWSVLGRH"
     gene            complement(331..3252)
                     /locus_tag="AC162G_17765"
     CDS             complement(331..3252)
                     /locus_tag="AC162G_17765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HIR5077707.1"
                     /translation="MPRIAVSLDLETTGLDPNRDAILEIGVVKFRGDEVLDEWSTLVD
                     PGRPIPHKITELTGITQAEVAAKGRPLFEALREAEHRIRDLPIVGHNVAFDLGFLNKQ
                     RVFTRNEFLDTFELASILVPHAGRYGLGALARELGIDLPAAHRALNDAKATHRLYVRL
                     AERARDVSAKTLQEILRLSASIDWPARAFFEDAARAAARGAFSGGSIGAQIAAKQPPR
                     RRRARPGGAVPELIAQSPMFARDAQIKPLRPNPVVEPIDTEMIAAALEQGGAFEQAID
                     HFEHRPQQVHMLRAVCDAFNRGEHLIVEAGTGTGKSLAYLLPAIHWAQRNGERVVVST
                     NTINLQEQLATKDAPDLQKALPLDFRTAVLKGRSHYLCPARLAAMLQSGPANADEMRV
                     LAKVLLWLPSTVSGDGDELFMPNPAERGAWARLSADNPTCTSERCSQWGDAGCFFHHA
                     RQEAEGAHVIIVNHALLLADVAVENRALPEYKYLIVDEAHHLEAATTDQLSFSINQYD
                     LRRTLEELSPLQRSRGPNLLDDAVGRVRSACPAEIASAVTVYGEQVSDGVAEADRMAE
                     EFFALVGDFVERHTEAKGDYTLRVRLTPAMRTQPAWSNVEIAWDNLNAQLNTVLDGLD
                     RLNRVLSDLADFGIPDYEEMTARLLGARRGLDEMRGSTNATVSKPSPGGIYWVESQPP
                     ASGRAGARPTISLHAAPLHVGPLVEEHLFKKKNSVVLTSATLRAGKSFDFIQGRLNAQ
                     DVDTLAVGSPFDYKSSTLLYLVTDVPEPNVNGYQRAIELGLIDLCTAMGGRTLALFTS
                     YSQLRATARVLAPELARRDIMVFEQSDGSSRRQLLDRFKASERGVLLGTRSFWEGVDV
                     PGEALSCLAITRLPFAVPNDPIFAARSETFDEPFMQYAVPDAVLRFRQGFGRLIRSRT
                     DRGVVVVFDRRIVSKQYGKVFLDSLPECTVRRGPIAELARAVAAWMKNHGGTDAQRTV
                     R"
     gene            complement(3322..3783)
                     /locus_tag="AC162G_17770"
     CDS             complement(3322..3783)
                     /locus_tag="AC162G_17770"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR5077708.1"
                     /translation="MADYYLDASALVKRYATEPGSAHILAITDVAAGNAVILAEITLV
                     EVASALAGKQRASRDFSLEDRDRALSRFLQECAEQFLLFRVGRDVIDQAVELTQKHRL
                     RAYDAVQLASALLANRILIEQNHPSLTFVVADADLLAAAQAEGLPIENPTQ"
     gene            complement(3776..4099)
                     /locus_tag="AC162G_17775"
     CDS             complement(3776..4099)
                     /locus_tag="AC162G_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077709.1"
                     /translation="MNTYTLTLDLPAKTYDSLQRWAKRKGVTPETLLNDIIERALQQT
                     SSASPSATRNILEAAGVLHSLSPALQKRIIPGVSLEEVHRSLDRAGGPSLSEMIRAQR
                     ERAVG"
     gene            complement(4149..4292)
                     /locus_tag="AC162G_17780"
     CDS             complement(4149..4292)
                     /locus_tag="AC162G_17780"
                     /inference="COORDINATES: protein motif:HMM:NF013122.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIR5077710.1"
                     /translation="MPRIAVSLDLETTGLDPNRDAILEIGVVKFRCEEVMGALVDRLI
                     PVC"
     gene            complement(4491..5510)
                     /locus_tag="AC162G_17785"
     CDS             complement(4491..5510)
                     /locus_tag="AC162G_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HIR5077711.1"
                     /translation="MPTHSLDDKRPHAFWDNSYPPRVKIKPGDTVIFETLEASANQIT
                     PKSTHDALKTLSFDPIHPLTGPVYVEGAEPGDALEVSIVSLKHKGWGWNAVIPGFGLL
                     ADDFPNPYLHHYKLGRTHCEFRPDIRIPYEPFCGVMGVAPREPGRFDTIPPRENAGNI
                     DIRHLTVGAKVLFPVLIPGALFSCGDCHSAQGDGEVNGTGIETPMTVRLRFDLKKGAN
                     IPELRFVTPPGKKLTIADEAGYFVTTAHGPDLFKDAQQAMRYMIDYLASEHDMTREQA
                     YCLCGAAVDLKISEIVDAPNWIVSAYLPLRIFVKPKEAPKRAAAKANARSKPKAKAAQ
                     KKARR"
     gene            complement(5593..6531)
                     /locus_tag="AC162G_17790"
     CDS             complement(5593..6531)
                     /locus_tag="AC162G_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077712.1"
                     /translation="MWIYIVRRVLQAVPVLLGISIITFVLIYYLPADPARMYAGPSAT
                     VETVERIRHRLGLDQPLWVQYTRYMERVLQGDLGFSYRKQIAVTELILSRIPYTLALT
                     FGGVLVELIIGLPVGIASAVARGRWMDRLGMFIALLGISAPPFWLGLLLLYWFGFRVP
                     IFPLGGAGSLAHLVLPALTAGLGGAAWYARMMRSSTLDILSAEYVRTARAKGLSTRGT
                     VIRHVLPNALNPIITMAGMDIPWFIGGVVLVERVFDWPGLGRLAVEAIETVDVPLILG
                     TVIVTSFIVVLSGILIDVVQGLADPRIRHSRLTASG"
     gene            complement(6534..7505)
                     /locus_tag="AC162G_17795"
     CDS             complement(6534..7505)
                     /locus_tag="AC162G_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008228605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077713.1"
                     /translation="MTVAQLDPSRGELSETDAANLAVPPVERALGEAGYFARAWRRLR
                     RDRSAMFGAMLVALIVAAALLADILAPYDPAEQFRDGLTPDGQPVPSTIVDGRARFLL
                     GTDANGRDLLSRILHGARVSLLVGVLANALAVTMGTLIGSVAGYFGGRLETLLMRFTE
                     IMMAFPTLLLAMTLVAILKPSVWIIIVVIGAVYWTWIARVVYGQVVALRDRDFVTAAR
                     ALGAGRRFILFRHILPQLVPTIIVWGTLGIATNVMIEASLSYLGIGVQPPTPSWGGMI
                     QQGQSFYRTAPWLVIFPGLSIMTTVFAFNLLGDGLRDALDPVQSGKL"
     gene            complement(7570..9204)
                     /locus_tag="AC162G_17800"
     CDS             complement(7570..9204)
                     /locus_tag="AC162G_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5077714.1"
                     /translation="MDKRAVFSTLVVLATVLAACGGAAPTAAPTDAPGAMTPQKGGEI
                     IVAYKDDLATLDPAIGYDWTNWPTEKMVFDGLLDYDSSTTITPRLADSLPEVSADATV
                     YTFKLRQGVKFHNGREVVAEDAAYSITRVLDPATGSPGAGFYLGILGAQEFVDGNATS
                     VEGIKALDPYTVQFTLSAPDVTFLNKMALNFAFIVPKEEVEKAGENFGHAPVGTGPYT
                     LKEWVSGQYLVFERNPNYFYEGLPYLDKITIQVGVAPDVALLRLEKGEIHLMGDPPPG
                     ADWARIAADPAWTDRLEIQPQVSTIYIAINTTAAPFDNPKVRQALNHAIDKQRIVQLL
                     NGRATVADQILPPLMPGYDEAYTGYEYNLDKAKALLAEAGYSSGFETSIECIAVDPQP
                     KLCESFQQDLANIGIKLTINTLAAPNVIEDAGNGKNPLTWSGGLAWIQDYPDPDDFYA
                     PILGCGSNVPGGWNWSRYCNEELHARSQELLGMTDRQTRLDAYKPFFKALMDDAVWVP
                     VVNGQYTIAHSDKLHGQPTLTHPEHLFVYETMWLSP"
     gene            9592..10530
                     /locus_tag="AC162G_17805"
     CDS             9592..10530
                     /locus_tag="AC162G_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5077715.1"
                     /translation="MTIAETAARIRANVQKVIVGKGEVIDLALVAVLCEGHILIEDVP
                     GIGKTTLAKALAASLGLSFRRIQFTPDLLPSDVTGVNVFNQKTQDFEFRPGPVFANVV
                     LADEINRATPRTQSAMLEAMQERQVTADGETRPLPRPFLVVATQNPIELEGTFPLPEA
                     QIDRFLVKLPLGYPSVEEEESILLRFQDDNPLDTLQAVASMDELAALPHEAHRVRVEA
                     SVRRYIVDLVRATRDREEVELGASPRGTLALYNAAQAYAAIQDRSFVLPDDVKRLVPY
                     VLTHRIILSPQMRLRGRRPEDVVAGVVENVPVPVEE"
BASE COUNT         1966 a         3314 c         3387 g         1899 t
ORIGIN      
        1 tcgtgcctcc ccagcacgct ccaccacagc ttcatcagcg actcgccgag cggccgatac
       61 gtaggcaggt tctcggcggt caggaaggga tcgacgaggc tgtgcgtgga cagccagcga
      121 atgtggatca cgtcatcttg gaagaacggc aggctgagcg tattcagata gagcagcgac
      181 gccaacccga tggcgagcgc aacgccggcg tacagcggcc agcgcggcgc gagtggggcg
      241 ggtgcgggga ctatccgagg cggggtgagg acgctcttca atcgcttcat gcacggtaag
      301 ccgccgagac accgaaagca cagagagctt tcaacgcaca gtgcgctgcg cgtccgtgcc
      361 tccgtggttt ttcatccacg ccgccaccgc gcgcgccagt tcagcgatcg ggccgcgccg
      421 cacggtgcat tcgggcagcg agtccaggaa caccttccca tattgcttgc tgacgatgcg
      481 gcgatcgaag accacgacga ccccgcgatc ggtgcgtgac cggatcaggc ggccgaaccc
      541 ttgccggaag cgcagcacgg catccggcac agcgtactgc atgaacggct cgtcgaacgt
      601 ctcgctgcgc gcggcgaaga tcggatcgtt cggcacggcg aacggcaggc gcgtgatcgc
      661 caggcacgac aacgcctcgc cgggcacgtc gacaccctcc cagaacgagc gcgtgccgag
      721 cagcacgccg cgctcggagg ctttgaatcg atcgaggagt tggcggcgcg acgagccgtc
      781 gctctgctcg aacaccatga tgtcacggcg ggccagctca ggcgccagca cgcgcgccgt
      841 cgcgcgcaac tgcgagtacg acgtgaacaa cgccagcgtg cgaccaccca tcgccgtgca
      901 caggtcaatc aggccaagct caattgcgcg ttgatagccg ttgacgttcg gctcgggcac
      961 atccgtcacc aggtacaaca gcgtggagga cttgtagtcg aacggcgagc caaccgccag
     1021 cgtgtccaca tcctgcgcgt tcaagcgacc ctggataaaa tcgaacgatt tgccggcgcg
     1081 cagcgtggcc gacgtgagca cgacgctgtt cttctttttg aacagatgct cctcgacgag
     1141 cgggccaacg tgaagcggcg cggcatgcag cgagatggtc ggacgcgcgc ccgcgcgccc
     1201 tgacgcaggc ggctgcgatt ccacccagta gatgccgccc ggcgacggtt ttgagacggt
     1261 cgcgttcgtg ctgccgcgca tctcgtcaag gccgcgacgc gcgccaagca ggcgcgcagt
     1321 catctcttcg tagtccggga tgccgaagtc ggcgagatcg ctcaataccc ggttgagccg
     1381 atcgaggccg tcgagcaccg tgttcaattg ggcgttcaag ttgtcccagg cgatctcgac
     1441 gttgctccag gcgggctgcg tgcgcatggc gggggtcagg cgcacgcgca gcgtgtaatc
     1501 gcccttcgcc tcggtatgac gctcgacgaa atcgccgacg agcgcgaaga actcttcggc
     1561 catgcgatcc gcttcggcga cgccgtcgct cacttgctcc ccatagacgg tcaccgccga
     1621 ggcgatctcg gcggggcatg cgctgcgcac gcggcctacc gcgtcgtcga gcaggttggg
     1681 gccgcggctg cgctgcagcg ggcttagctc ctcaagcgtg cggcgcaggt cgtactggtt
     1741 gatggagaag ctgagctggt cggtcgtggc ggcttcgagg tggtgcgcct cgtcgacgat
     1801 caggtacttg tattcgggca gcgcgcggtt ctcgacggcg acgtcggcaa gcagcaaggc
     1861 gtgattgaca atgatcacgt gcgcgccctc ggcttcctgc cgcgcatggt ggaagaagca
     1921 gccggcgtcg ccccactgcg agcagcgctc ggacgtgcag gtcgggttat cggcgctcag
     1981 gcgcgcccat gcgccgcgct cggccgggtt gggcatgaac agctcgtcgc cgtcaccact
     2041 caccgtcgac gggagccaca ggagcacctt ggccagcacg cgcatctcgt cggcgttggc
     2101 cgggccggac tgcagcatcg cagccaggcg cgcggggcat aggtaatgcg agcggccttt
     2161 cagcacggcg gtgcggaaat cgagcgggag cgctttttgc agatcgggcg cgtccttcgt
     2221 cgcaagctgc tcctggaggt tgatggtgtt cgtcgagaca accacgcgct cgccgttgcg
     2281 ctgggcccag tgaatcgcgg gcagcagata agccaatgac ttgccggttc ctgtgccggc
     2341 ctcgacgatc agatgctcgc cgcgattgaa cgcgtcgcat acggcgcgca gcatgtgcac
     2401 ctgttggggc cggtgctcga agtggtcgat tgcttgctcg aacgccccac cctgctccag
     2461 cgccgccgcg atcatctcgg tgtcgatcgg ctcgacaacg gggttgggtc gcaagggctt
     2521 gatctgcgcg tcccgcgcga acatcggcga ctgcgcgatc agctccggca cggcgccgcc
     2581 tggcctggcc cgtcgccgcc tggggggctg cttggcagca atctgcgcgc cgatgctccc
     2641 accgctgaac gcgccgcgcg cggccgcgcg cgcggcatcc tcgaagaaag cgcgcgccgg
     2701 ccagtcgatc gaggcggaga gccgaaggat ctcttgcagc gtcttggcgg acacatcgcg
     2761 ggcgcgctcg gcgagcctga cgtacagccg gtgtgtcgcc ttcgcatcgt tcagggcgcg
     2821 gtgtgcggcg ggcagatcga tgcccagctc gcgcgccagt gcccccagcc cgtaccgccc
     2881 ggcgtgcgga accaggatgc tcgccagctc gaacgtgtcg aggaactcgt tacgggtgaa
     2941 gacgcgctgc ttgttgagga atccgaggtc gaatgcgacg ttgtggccga cgataggcag
     3001 atcgcggatg cgatgctccg cctcgcgcag cgcttcaaac agcggcctgc ccttcgcggc
     3061 gacttccgcc tgcgtgatgc cggtgagttc ggtgatcttg tgcgggatgg gccggcccgg
     3121 atcgacgagc gttgaccact cgtccagcac ttcgtcgcct cgaaacttga ccacgccgat
     3181 ctcgaggatc gcgtcccggt tcggatcgag gccggtcgtc tccaggtcca ggctgacagc
     3241 gatgcgtggc acggagggga gttcccgttc taggcgctgt gttggttcgg cgcaaagggg
     3301 attataccac gcggatcacg ttcactgcgt gggattctct atcggcaatc cctcagcttg
     3361 agcagcggcg agaagatcgg catctgcgac gacaaatgtg agagaggggt ggttctgttc
     3421 tatcagaatg cggttggcga gaagcgcact cgctagctgg acagcatcgt atgcacgcag
     3481 gcgatgcttt tgggtcagtt caacggcctg atcgattacg tcgcgtccaa cacggaagag
     3541 gagaaattgt tcggcgcact cctggagaaa gcgactgaga gcccgatctc gatcttccag
     3601 gctgaagtcg cgcgatgcgc gttgcttgcc ggccagtgcc gaggcaactt cgaccagcgt
     3661 gatctcggcc aagatgaccg cgttgccggc agcgacatcg gtaatcgcca ggatatgtgc
     3721 gctaccaggc tcagtcgcgt agcgcttcac caacgcgcta gcatcaagat aataatcagc
     3781 caacggcacg ctcgcgctgg gcgcggatca tttcactcaa agacggtccg ccggcccgat
     3841 cgagagacct gtggacttct tccaacgaga cgccgggaat gatccgtttc tgcaaagccg
     3901 gactgaggga gtgcagcacg cccgcggctt ctaatatatt gcgcgtagct gaaggagacg
     3961 cagaggacgt ctgttgaagc gctcgctcga taatatcgtt caggagggtt tcaggtgtta
     4021 caccctttct tttagcccag cgttgcaagg aatcataggt cttagctggc aagtctagcg
     4081 taagggtata cgtattcatc gccacatctc cggtgtcagc aacgaagtcc tgattatcca
     4141 acagtattct agcacaccgg tattagccgg tcaaccaagg cccccatcac ttcctcgcag
     4201 cgaaacttga ccacgccaat ctcgaggatc gcgtcccggt tcggatcgag gccggtcgtc
     4261 tccaggtcca ggctgacggc gatgcgtggc acggagggga atccgttctg ggcgcccggc
     4321 tgcgcggcgc gagcggatta taccatcgaa acctgatgtg tttgctcgtc aaactcagtc
     4381 gcgctatgac tgtccccaaa ctcagctata ttcctacggt tactggagtc ccctggctca
     4441 tccacgtgtt ctaacaaccc gggccactcg atcgagtggc ccagggttct ttatcgtcgt
     4501 gctttcttct gtgccgcctt cgcctttggc ttggacctgg cgttcgcctt agccgcagcg
     4561 cgcttgggcg cctctttcgg cttgacaaaa atgcgcaggg gcaggtaggc cgacacgatc
     4621 cagttcggcg cgtcgacgat ctcgctgatc ttcaagtcga ccgccgcgcc gcacaggcag
     4681 tacgcctgct cgcgggtcat gtcgtgctcc gaggccagat aatcgatcat atatcgcatg
     4741 gcctgctgcg cgtccttgaa caggtcgggg ccatgcgcgg tggtgacgaa gtagccagcc
     4801 tcgtcggcga tggtaagttt cttgccgggc ggcgtgacga agcgcagttc ggggatgttc
     4861 gcgcctttct tcaggtcgaa gcgcagcctc accgtcatcg gcgtttcgat gccggtgccg
     4921 ttcacttcgc cgtcgccctg cgccgagtga cagtcgccgc atgagaacag cgcgccagga
     4981 atcagaaccg ggaagagcac tttcgcaccg acggttagat ggcggatgtc gatgttgccg
     5041 gcgttttcgc gcggcgggat ggtgtcgaac cgccccggct cgcgcggcgc gacgcccatc
     5101 acgccgcaga atggctcata ggggatgcgt atgtccggcc tgaactcgca gtgcgtccgc
     5161 ccgagcttgt aatggtgcag gtagggattg gggaagtcat cggccagcag gccgaaaccg
     5221 gggatgacgg cgttccaacc ccagcctttg tgcttaaggc tgacgatgct gacctccagc
     5281 gcatcgccgg gttccgcgcc ctcgacgtac accgggccgg tgagcggatg gatcggatcg
     5341 aagctcagcg tcttcaacgc atcgtgcgtt gacttcggcg tgatctgatt ggccgaggct
     5401 tccagagtct cgaagatcac ggtgtcgcct ggcttgatct tgacacgcgg cggatacgag
     5461 ttatcccaaa aggcgtgcgg tcgtttgtcg tcgagagagt gcgtgggcat gtgtgttacc
     5521 tcctcacaga gaatggattg ggggctgaga atccggatcg ggcttcaagc gaatcaatca
     5581 tccattgccg cttcagccgc tggccgtcag acggctgtgg cggatacgtg gatcggccag
     5641 gccctgcacg acatcgatca gaatgccact gaggacgacg atgaaagagg taacgatcac
     5701 cgtccccaga atgagcggga catccaccgt ttcgatggcc tcgacagcca ggcgtcccag
     5761 tcccggccag tcaaacacac gctcgaccag cacgactccg ccgatgaacc agggaatgtc
     5821 catgcccgcc attgtaatga tcgggttcag ggcattgggc aggacatgcc ggatgacggt
     5881 tccacgggtg gagaggccct tggcgcgcgc cgtgcggaca tactcggccg agagaatatc
     5941 gagcgtgctg gagcgcatca tgcgtgcgta ccaggctgct ccacccagcc cggccgtgag
     6001 cgccggcaga acaaggtggg ctaggctgcc tgcgccaccg agtgggaaga tcggaactcg
     6061 gaaaccaaac cagtacagca gcagcaggcc cagccagaac ggcggggccg agatgccgag
     6121 caacgcaatg aacatcccca accgatccat ccaccgacca cgcgccaccg ctgacgcaat
     6181 ccccaccggc agcccgatga tcaactcgac caggacgccg ccgaaggtga gcgccagcgt
     6241 atacggaatg cggctgagga tcagttcggt gaccgcgatc tgcttgcgat acgaaaagcc
     6301 cagatcgcct tgcaggacgc gctccatgta gcgcgtgtac tgcacccaca acggctgatc
     6361 caggccaagt ctgtgccgaa tacgctccac cgtttcgacg gtcgcgctcg gcccggcgta
     6421 catacgagcc ggatcggccg gcaagtaata gatcagcacg aacgtgatga tcgagatgcc
     6481 gagcagcacg gggacagcct ggagcacgcg gcgaacgata tagatccaca tcgtcatagc
     6541 ttcccgctct ggaccggatc gagcgcatcg cgcagcccgt cgcccagcag attgaaggca
     6601 aacacagtcg tcatgatcga tagaccggga aagatcacca accagggcgc ggtgcggtag
     6661 aacgactggc cctgctggat catcccgccc cagcttggcg taggcggctg cacaccgatg
     6721 ccgagatagc tcaggctggc ttcgatcatc acattcgtgg cgatgccgag tgtgccccag
     6781 acgatgatcg tgggaaccag ttgaggcagg atgtgccgga acaggatgaa ccggcgaccg
     6841 gcgcccaggg cgcgcgccgc cgtcacgaag tcacggtcac gcagcgccac cacctgcccg
     6901 tacacgacgc gcgcaatcca cgtccagtac accgcgccga tcacgacgat gatgatccac
     6961 acgctgggct tgaggatcgc gacgagcgtc atcgccagca gcagcgtggg gaacgccatc
     7021 atgatctcgg tgaagcgcat gagcagcgtc tccagccgcc caccgaagta gccggcgacc
     7081 gaaccgatca atgtgcccat cgtcacggcc agcgcattcg ccagcacacc aaccagcagg
     7141 ctgacgcgtg ccccatgcag gatgcggctg agcagatcac gcccgttcgc atcggtgccg
     7201 agcaggaagc gcgcacggcc gtcgacgatc gtactcggca cgggctgccc gtccggcgtc
     7261 agcccatctc gaaattgctc ggccggatca tacggcgcga ggatgtctgc caggagcgcc
     7321 gccgccacaa tcagcgcgac gagcatcgca ccaaacatgg ccgagcgatc gcgccgcagg
     7381 cgacgccagg cccgcgcgaa ataccctgcc tcgcccagcg cccgctcgac aggcggcacg
     7441 gccaagttcg ccgcgtcggt ctcagagagc tcccctcggc tgggatcaag ttgggcaact
     7501 gtcataggct ggagtcgggt cgggggcagg tgcgaagcac cacgccggcg cgtcgcacct
     7561 gcagattgtc tatggcgaca gccacatcgt ttcgtagacg aagagatgct ccggatgagt
     7621 gagcgtcggc tggccgtgca gcttgtcgga atgggcgatg gtgtactggc cattaaccac
     7681 cggcacccac accgcatcgt ccatcagtgc cttgaagaag ggcttgtagg catccaggcg
     7741 cgtctgacga tcggtcatgc cgagcaactc ctggctcctg gcatggagtt cctcgttaca
     7801 atagcgcgac cagttccagc cacccggcac attcgagcca cagccgagga ttggcgcata
     7861 gaagtcatcg gggtcaggat agtcctgaat ccaggccagc ccacccgacc aggtcagcgg
     7921 gttcttgcca ttgccggcgt cttcgatcac gttcggcgcg gccagcgtgt tgatggtgag
     7981 cttgatcccg atattggcta ggtcttgctg gaaggactca cacagcttgg gctgcggatc
     8041 gaccgcaatg cactcgatgc ttgtctcgaa gccgctggag taaccggctt ccgccagcag
     8101 tgccttggcc ttgtcgaggt tgtactcata gccggtatag gcttcgtcat agccgggcat
     8161 gaggggcggg aggatctgat ccgccactgt ggcgcggcca ttgagcagct gcacgatgcg
     8221 ctgcttgtcg atcgcgtggt tgagcgcctg gcgcaccttc gggttgtcaa acggcgcagc
     8281 ggttgtgttg atggcgatat agatcgtgct gacctggggc tgaatctcca ggcgatctgt
     8341 ccacgctgga tccgctgcga tgcgtgccca gtcagcgccg ggcggtgggt cgcccatcag
     8401 gtggatctcg cctttctcca gccgcaatag cgccacatcg ggcgcgacgc cgacttgaat
     8461 cgtgatcttg tcgaggtagg gcagaccttc gtagaagtag ttcgggttac gctcgaacac
     8521 caggtactgg cccgacaccc attctttgag agtatatggg ccggtgccga ctggagcgtg
     8581 gccgaagttt tcgccggctt tttcgacctc ttccttaggc acgatgaagg cgaaattgag
     8641 ggccatcttg ttcaggaaag tcacatccgg cgcgctcagt gtgaactgaa cggtatacgg
     8701 gtcgagggcc ttaatccctt ccaccgaagt ggcattgccg tcaacaaact cctgcgcgcc
     8761 caggatgccc aggtagaatc cggcaccggg gctgccggtc gccggatcga gcacgcgtgt
     8821 gatactgtag gcagcgtcct cggcgacgac ttcgcggccg ttgtggaact tgacaccctg
     8881 acgcagtttg aaggtgtaca ccgtcgcgtc ggcactgacc tcgggcaaac tgtcggccag
     8941 acgcggcgtg atcgtcgtgc tgctgtcgta gtcgagcagg ccgtcgaaga ccattttttc
     9001 ggtcggccag ttcgtccagt cgtaaccgat cgccgggtcg agcgtggcca gatcgtcctt
     9061 ataggccacg atgatctcgc ctcccttctg cggtgtcatc gccccgggcg catcggtcgg
     9121 cgcagcagtc ggcgctgcgc ctccgcaggc tgcgagcaca gtagccaaaa caacgagtgt
     9181 gctgaaaacg gcacgcttgt ccatcgacat tctcctccct cgattgggtt catagcggga
     9241 cgccgtgcgc tggcgcgccc cttcagccag gcaccacaac cagtcgaaga tgctcgggtg
     9301 cggcacctcc tttcgtgctc tctgatgtcg gaatgcagga acggccagat tatatcacat
     9361 ctcgcgtcgg atcaaggcat ggccgtttgg tgattgcacg catctcgctc cgcgctgtcc
     9421 cttcgacagg ctcagggcag gcctcggcgc gggcggtagc caccggggac ggcggcttga
     9481 gcacttgtgg ataatcgcca gatgaccgtc ccttccatgc tatacttgct ttggcctgaa
     9541 ccttttcatc catcctatcg tagatcagtg agcccaattt ccgaggtgtg catgaccatc
     9601 gccgaaaccg ccgcccgcat ccgcgccaac gtccaaaaag tgatcgtcgg caaaggcgag
     9661 gtcatcgatc tcgcgctcgt cgccgtcctg tgcgaaggac acatcctgat cgaggatgtg
     9721 cctggcatcg gcaagacgac gctggccaag gcgctggctg cctcgctggg gttgagcttt
     9781 cggcgcatcc agttcacgcc cgacctgctg ccgagcgacg tgacgggcgt caacgtcttc
     9841 aaccagaaaa cgcaggactt cgagtttcgt cccggcccgg tcttcgccaa cgtcgtcctg
     9901 gccgacgaga tcaaccgcgc cacgccgcgc acgcagtcag cgatgctcga agcgatgcag
     9961 gagcgacagg tcaccgccga cggcgaaacg cgtcccctgc cccgcccgtt cctggtcgtc
    10021 gccacacaga acccgatcga gctggagggc acgttcccgc tgcccgaggc gcaaatcgac
    10081 cgcttcctgg tcaagctgcc attgggatat cccagcgtcg aggaagagga atcaatcctc
    10141 ttgcgctttc aggacgacaa cccgctggac acgctgcagg cggtcgcgtc gatggacgag
    10201 ctggccgcgc tgccccacga agcgcacagg gtgcgcgtcg aagcgtcggt gcgccgctac
    10261 atcgtcgacc tggtgcgcgc gacgcgagac cgcgaggagg tggagctggg cgccagcccg
    10321 cgcggcacgc tcgcgctgta caacgccgcc caggcatacg cggcgattca ggatagatcg
    10381 ttcgtgctac cggacgacgt caagcgcctg gtcccgtacg tgctgacgca tcggatcatc
    10441 ctcagcccgc agatgcgcct gcgcgggcgg cggcccgagg acgtcgtcgc gggcgtcgtc
    10501 gagaacgtgc cggttcctgt ggaggagtag agagtagtaa ttggagattg gtaattggag
    10561 attagc
//
LOCUS       DBNHXB010000453         2911 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1149683, whole genome shotgun sequence.
ACCESSION   DBNHXB010000453 DBNHXB010000000
VERSION     DBNHXB010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1149683"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="AC162G_17810"
     CDS             complement(<1..242)
                     /locus_tag="AC162G_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5077716.1"
                     /translation="MSERILVVDDDREIVRLVRAYLEQAGYYTLTAYNGETALHIIRR
                     DRPDLIVLDLMLPDRDGWSITRIVRGDESLARTPII"
     gene            515..>2911
                     /locus_tag="AC162G_17815"
     CDS             515..>2911
                     /locus_tag="AC162G_17815"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5077717.1"
                     /translation="MTDDPLQTARLPRVDVRAFLPAPLAERWGRDADAGARRAVLDSL
                     DHLLGALTTYLPRHLLHELMLRPEPGRTHGAFLSSTVLFADVSGFTAMSERLAALGRE
                     GAETLTGVINDYFTTMSDIAAQLGGDLMLFGGDAMLLLFDGADHALRACHAAWRMQRA
                     MRERFAEVETALGAFPLHMAVGLGSGPVFAAALGSADSMHYAVMGPALTAMGHAEALA
                     GAGRIIMDASTRLLAGDLAVDAEGDSGFFGLTAEPTAPYTRPDPPVLDLPPVAEDLRL
                     DWLAQRLAAIAPFLPPGLLPRLLPDPAALLEGEHRPVTVLFIDFRDADALIDILGADD
                     PTALTPRLNAAFNTMRSIVARYEGVINKIGGGPAGPHVIALFGAPVSHEDDAERAVHA
                     ALEIGAALSGADLPGVHPARIGINTGFVYAANVGSHVRREYTVMGDQVNLASRLMTAA
                     QAAQIVVSASTAGHVEHKFDLEERDPVTVKGKSQSQRNFLVRGARRREVKTAAFGELL
                     GRSDALDTVRARIAAAAQGRGQIVAFTGEAGVGKSRLVETLAEQAAAEGFDVVAGACL
                     SYGRDTPYSVWAEPLRAIIGVQPSAAEAERRARLAASLQAIDRVDDLPIVAEIVGVPA
                     VDTGWTASLDARARQRRLFDAVAGLIRGRARRAPLMLVIDDAQWIDSASLDLLNTLAA
                     RINDAAIIVIVASRAERRWTGWDGLPNAVEIVLPPLERAAIEAMIADLLDIGDRRLPL
                     AVSTLLERMAAGVLSTAPVDASTFERYWGNPFFAQERVRALIDSGVLVRESASVSSAA
                     L"
BASE COUNT          426 a         1051 c          929 g          505 t
ORIGIN      
        1 ataatgatcg gtgtgcgagc cagcgactcg tccccacgca cgatgcgcgt gatgctccag
       61 ccatcgcggt cgggcagcat caggtcgagc acgatcaggt caggccggtc gcggcgaatg
      121 atgtgcaacg ccgtctcacc gttgtaagct gtgagggtgt aatagccggc ctgttcgaga
      181 tacgcccgca ccaaccgcac gatctcccta tcgtcgtcta cgaccaagat acgctcactc
      241 accgtcaccc ttctcagaaa tcaggtttct caaagaaacc tggtttcttt agaaggatac
      301 cacagacttg tggagaaatc atggagaccc ggcgtatcag agcggcggat tgtcatccga
      361 cttgagcggc ctgacagttt tgcggcatac ccggagtatc ggaagtattc caagagtacc
      421 ggtctggtta cagcgtccgt cgccgggttg accgttttgc aacctaagtg tattctggtt
      481 ctggcgctgc cggccacagc gtaaccgtgc cgctatgact gacgatcctc tccaaaccgc
      541 acgcctcccg cgcgtcgacg tccgcgcatt tctgcccgcg ccgctggccg agcgctgggg
      601 acgcgacgca gatgccggcg cgcgccgcgc cgttctcgat tcgctcgatc acctgctcgg
      661 cgcgctgacg acctaccttc cccggcatct gcttcacgag ctgatgctgc gccctgagcc
      721 tggccgcact cacggggcct tcctgtcgag caccgtcctg ttcgccgacg tctccggctt
      781 caccgctatg tccgagcgat tggccgcgct cggccgtgag ggcgccgaga cgctcaccgg
      841 cgtcatcaac gactacttca cgacgatgag cgatatcgcc gcgcagctcg gcggcgatct
      901 gatgctgttc ggcggcgacg ccatgctctt gctcttcgac ggcgccgacc acgcgctgcg
      961 cgcctgtcac gccgcctggc gcatgcagcg cgccatgcgc gagcggttcg ctgaagtcga
     1021 gacggcgctc ggcgccttcc cgctgcacat ggccgtcggc ctgggcagcg gccccgtctt
     1081 tgccgccgca ctcggatcgg ccgatagcat gcattacgcc gtcatggggc cggcgctgac
     1141 cgcgatgggg cacgccgagg cgctcgccgg cgcgggacgc atcatcatgg acgcttcgac
     1201 gcgcctgctc gccggcgacc tggccgtcga cgcagaaggc gactcgggtt tcttcggcct
     1261 gacggcagag ccaaccgcgc cctacacccg gcccgatccg cccgtgctcg atctaccgcc
     1321 cgtcgccgaa gacttgcggc tcgactggct ggcgcagcgg ctggccgcca tcgcgccctt
     1381 cctgcccccc ggcctgctcc cgcgcctcct gcccgatccg gcggccctgc tcgaaggtga
     1441 gcaccgcccg gtcactgtgc tgttcatcga cttccgcgac gccgacgcgc tgatcgacat
     1501 cctcggcgcg gacgatccca ccgcgctcac gccgcgcctg aacgcggcgt tcaataccat
     1561 gcgctcgatc gtcgcccgtt acgagggtgt catcaacaag atcggcggtg ggccggccgg
     1621 cccgcacgtc atcgcgctct tcggcgcgcc ggtcagccac gaggacgatg ccgagcgcgc
     1681 cgtgcacgct gcgctggaaa tcggcgcggc gctgtccggc gcagacctgc ccggcgtgca
     1741 tcccgctcgc atcgggatca acaccggctt cgtgtatgcc gccaacgttg gatcgcacgt
     1801 gcgccgcgag tacacggtca tgggcgacca ggtcaacctg gcatcgcgcc tgatgacagc
     1861 cgctcaggcg gctcagatcg tcgtctcggc gtcgactgcc ggacatgtcg agcacaagtt
     1921 cgatctcgaa gaacgcgatc ctgtgacagt gaaaggcaag tcgcagtcgc aacgcaactt
     1981 cctggtgcgg ggcgcgcgcc gccgcgaggt gaagacggcg gccttcggcg agttgctcgg
     2041 ccgctcggat gcgctcgata ccgtccgcgc gcgcatcgcc gccgccgcgc agggccgcgg
     2101 ccagatcgtc gcgttcaccg gcgaagccgg cgtcggcaag tcgcgcctgg tcgagacgct
     2161 cgccgaacag gcggccgccg agggattcga cgtcgtcgcc ggcgcgtgcc tctcctacgg
     2221 ccgcgacacg ccgtactccg tgtgggccga gccgctgcgc gcgattatcg gcgtgcagcc
     2281 ttcggccgcc gaggccgagc ggcgcgccag gctcgcggcc agcctgcaag ccatcgaccg
     2341 cgtcgacgac ctgcccatcg tcgccgaaat cgtcggcgtc ccggccgtcg acactggctg
     2401 gaccgcgtcg cttgacgcgc gggcccgcca gcgccgcctg ttcgatgccg tcgccggtct
     2461 catccgtggc cgcgcgcgcc gcgcgccgct tatgctcgtc atcgatgacg cccagtggat
     2521 cgattccgcg tcgctcgatc tgctgaacac cctggccgcg cgcatcaatg acgccgccat
     2581 cattgtgatc gtcgcctcgc gcgccgagcg ccgatggact gggtgggacg gattgccgaa
     2641 tgccgtcgag atcgtcctgc cgccgctcga gcgcgccgcg atcgaggcga tgatcgccga
     2701 tttgctcgac attggcgacc ggcgcctgcc gctcgccgtc agcacgctcc tagagcgtat
     2761 ggcggccggc gtgctcagca ctgcgccggt ggatgcgtcc accttcgagc gctactgggg
     2821 caacccgttc ttcgcgcagg agcgcgtgcg cgcgctgatc gattccggcg tgctggtgcg
     2881 tgaatcggca tctgtctcct ccgctgcgct t
//
LOCUS       DBNHXB010000454         3069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3836067, whole genome shotgun sequence.
ACCESSION   DBNHXB010000454 DBNHXB010000000
VERSION     DBNHXB010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3836067"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="AC162G_17820"
     CDS             complement(<1..950)
                     /locus_tag="AC162G_17820"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR5077718.1"
                     /translation="MASETIFLINYRNHRDIYPPFGIMYVADALMQAGFNVKLFHDTG
                     EFVEPFVQKVAAEKPLFVGFSTITGPQLRPTIEASKKVHALGVPVVWGGVHATIMPLD
                     VLKEDYVDFVVVNEGEETVQEFAQMLAGDTPPLWQGVAGLAYKDAGGTPMFHHERPFI
                     DGVDRFRPRWELIDVERYLIPSGPYSRAIPVYISRGCPFRCGFCYNEVVMKRTWRQHS
                     DAFILDQIQWLKDNYRIEAVDYADDYLFGRIKPMQRLVEKVGMPWSGQVRVQLLSPEF
                     VKWMRDTGCQWVNIGAESGSQAVLDNMHKDQKAWQIEWGMR"
     gene            complement(1070..2584)
                     /locus_tag="AC162G_17825"
     CDS             complement(1070..2584)
                     /locus_tag="AC162G_17825"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR5077719.1"
                     /translation="MRFLLINPPIGVFYEAVVPPIGLAYLAAALRQAGHQAEILDINL
                     WRWPPDEVRRRVAASDADAFGITGLISQYTYNKWLADTIKDAHPDRPVIQGGMMVSAM
                     PQIMLEESPSTDFVVVGEGEQTLVELADALEGHRPLQSVKGLWYHDPAKSGKLIVTDY
                     RPDMPDIDPLPYPAWDLVDIPAYWQSRARAREMHLIYKNKPFIPLLATRGCPYRCNYC
                     HRFADDRLRQRSTESVLAEIDHWRTIYNVEYVDFQDELWGLSKPRAKEFCQALKATGW
                     NITWGCSIRLNVIDEEMIRMMVESGCVFVSFGVESGSEEILINMNRRMKREVIVQGWN
                     IVKKYQLTHLPSFMIGYTGETPETIRETVDLVCQMDVGSFAGWFRYVTPLPGTQLYEY
                     AKVKGLIRDEKAYIEEAGKTFAGLTTFDKLLVNLTEMSDEELRDWREWGRREILRHHL
                     RRHPIRYPLQLVHDGFNYVRRHGVRQTWRHIPEIIRQHLPEASPYHLPVGAAPD"
     gene            complement(2684..>3069)
                     /locus_tag="AC162G_17830"
     CDS             complement(2684..>3069)
                     /locus_tag="AC162G_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077720.1"
                     /translation="GHTHHLSAAQRAIGDATIALGPQPARKWAGLSPIGTALSIVLAS
                     RGRRDLAAGAAILGMFGSAFGLAGFRRPERTLEITGREALRSFGIGAAIGLVLLTLRN
                     AGRTVTTQPTQLSGVEALAGRVRQT"
BASE COUNT          551 a          945 c          935 g          638 t
ORIGIN      
        1 cgcatgcccc actcgatctg ccacgccttt tgatctttgt gcatgttgtc gagcacggcc
       61 tgcgatcccg attccgcgcc gatattcacc cactggcagc cggtgtcgcg catccacttg
      121 acgaactcgg gcgagagcaa ctgcacgcgc acctggccgc tccacggcat gcccaccttc
      181 tcgacgagcc gctgcatggg cttgatgcga ccgaagaggt aatcgtcggc atagtctacc
      241 gcctcgatgc ggtagttgtc cttcagccat tgaatctggt cgaggatgaa cgcgtcggag
      301 tgctgccgcc atgtgcgctt catcacgact tcgttgtaac agaagccaca gcggaacggg
      361 cagccgcgcg agatgtagac cgggatcgcc cggctgtacg gcccactggg aatgagatac
      421 cgctcgacgt cgatcaattc ccagcgcggc cggaagcggt cgacaccgtc gatgaagggc
      481 cgctcgtgat gaaacatcgg cgtgccgccg gcgtccttgt aggccagccc cgccacgccc
      541 tgccagagcg gtggcgtgtc gcccgccagc atctgtgcga attcctggac ggtctcctcg
      601 ccctcgttga cgacgacgaa atcgacgtag tcttccttca gcacgtcgag cggcatgatt
      661 gtggcgtgca cgccgcccca cacgaccgga acgccgagcg cgtgaacttt cttcgaggcc
      721 tcgatcgtcg gcctcaattg cgggccggtg atcgtgctga acccgacgaa caggggcttc
      781 tcggcggcga ctttctggac gaacggctcg acgaattctc ccgtgtcgtg aaatagcttg
      841 acgttgaacc cggcctgcat cagcgcgtcg gcgacgtaca tgatgccgaa cggcggatag
      901 atgtcgcggt ggttgcggta gttgatcaag aagatcgttt cgcttgccat gctctccccc
      961 ggttggccgg taatcagtta tcagtaatca gttgtcagta atcagttgtc agtgaacagt
     1021 gatcactgat tactggtcac tgatcactga tcactacagg tggtagccgt tagtcaggcg
     1081 ccgcgccgac cggcaggtgg tatgggcttg cctctggcaa atgctgccgg ataatctcgg
     1141 ggatgtgccg ccacgtctgg cggacaccgt ggcgtcggac gtaattgaac ccatcgtgca
     1201 ccagttgcag cgggtatctg attgggtggc ggcgcaggtg atggcgcagg atttcgcgcc
     1261 ggccccattc gcgccagtcg cgcagctctt cgtcagacat ctcggtcagg ttgaccagaa
     1321 gcttgtcgaa ggtggtcagc ccggcgaagg tcttgcccgc ttcttcaatg tacgctttct
     1381 catcccgaat cagccccttc accttggcgt actcgtacag ttgcgtgccg ggcagcggcg
     1441 tgacgtagcg gaaccagccg gcgaaagagc cgacgtccat ctggcacacc agatcgaccg
     1501 tttcacggat ggtttccggc gtctcgccgg tataaccgat catgaacgaa ggcagatggg
     1561 tcaactggta tttcttgacg atgttccagc cttgaacgat gacttcccgt ttcatgcgcc
     1621 ggttcatgtt gatcaggatc tcttcgctgc cggattccac gccgaaggag acgaagacgc
     1681 aaccggactc gaccatcatg cggatcatct cttcgtcgat cacgttcagt cgaatcgagc
     1741 agccccaggt gatgttccaa ccggtggctt tgagcgcctg gcagaattct ttggcccgcg
     1801 gtttggacaa gccccatagc tcatcctgga agtcgacgta ctcgacgttg tagatggtgc
     1861 gccagtgatc gatctcagcc agcaccgatt cggtgctgcg ctggcgcagg cggtcgtcgg
     1921 caaagcgatg acagtagttg cagcggtacg ggcagccgcg cgtcgccagc agcgggatga
     1981 atggcttgtt cttgtagatc aggtgcatct cgcgggcgcg cgcccggctt tgccagtaag
     2041 ccgggatatc caccaggtcc caggccggat acggcagcgg gtcgatgtcc ggcatgtcgg
     2101 gtcgatagtc ggtgacgatc aacttgccgg acttcgccgg gtcatggtac cacaacccct
     2161 tgacactctg gagtggtcga tgtccttcca gtgcgtcggc cagttcgacg agcgtctgct
     2221 cgccctcgcc gacgacgacg aagtcggtgc tcgggctttc ctccagcatg atctgtggca
     2281 tggcgctgac catcatgcca ccctggatca ccggccggtc gggatgcgcg tccttgatag
     2341 tgtcggccag ccatttatta tacgtgtact gcgagatcag gccggtgatg ccaaaggcgt
     2401 cggcgtcgct ggccgcgaca cggcggcgca cttcatccgg tggccagcgc cataggttga
     2461 tgtccaggat ttccgcctgg tgccccgcct gtcgcagcgc ggctgccaga taggcgaggc
     2521 cgatcggcgg cacaaccgcc tcgtagaaca cgccgatcgg tggattgatc aacaggaatc
     2581 tcatgtcagc ctccccccac tgatattggt tccacctacc cgattttcaa agtgcggcgt
     2641 aactgctcag cccttcgtgt tggaatagag gctttagccg gtttcatgtc tggcgcactc
     2701 ttccggctaa agcctcgact ccggacagct gggtgggttg agtagttaca gtgcggccag
     2761 cattgcgcag agtgagtaag acgagtccta tggctgcccc gattccgaag ctcctcagcg
     2821 cttccctccc cgtgatttca agcgtgcgtt ccggcctgcg gaagccggcc aacccaaagg
     2881 cactcccgaa catgccgagg atggctgctc ctgcggccag gtcacgacgg ccccggctcg
     2941 ctagaacgat gctcaacgcg gttccgatgg gcgacaggcc cgcccacttg cgcgcgggct
     3001 gcgggccgag cgcgatcgtc gcatcgccga tcgcgcgctg cgctgccgac agatgatgcg
     3061 tgtgcccct
//
LOCUS       DBNHXB010000455         4866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_314369, whole genome shotgun sequence.
ACCESSION   DBNHXB010000455 DBNHXB010000000
VERSION     DBNHXB010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_314369"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="AC162G_17835"
     CDS             complement(<1..554)
                     /locus_tag="AC162G_17835"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5077721.1"
                     /translation="MNLAGRDLGQYHVVELIGQGGMAAVYKAYQPALERHVAIKVLPE
                     QLALVPDFSDRFVREAKAIAQLNHPNILPVMDFGQEDGLSYIVMKYVRGGTLADRLRR
                     PIDLLTTTRLVEQIAAALDHAHSRGIIHRDVKPSNVLLDEGEWVQLADFGLAKIVARD
                     EDLTGTGTGMGTPTYLSPEQGKGLP"
     gene            complement(615..2360)
                     /gene="polX"
                     /locus_tag="AC162G_17840"
     CDS             complement(615..2360)
                     /gene="polX"
                     /locus_tag="AC162G_17840"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HIR5077722.1"
                     /translation="MNNQDVARTFETLADMLDIEGQENKFKIIAYRRVAEQIANLDRD
                     VNVLRRAGELRSIPGVGQAIEEKIDSLLRTGTFSLYERLQKEIPAGVVAMLAIPDLGP
                     KRAKLLWKEAGITSVEELGKKARAGELAGLPGLGRKFEEKIVANIEAMRRRGASGRSP
                     LGIAYPLARDMLRRLMAVAGVQRASVAGSLRRMRETIGDIDLLVSAEPADAEHIMDAF
                     NALPNVAEVVARGPTKSSVRLHTGQQVDLRVIQPRHWGAALQYFTGSKDHNVRLREIA
                     LKKGYSLNEYSLTRVKDGKDFFCREEAEVYAKLGVPFMPPELREDRGEFEDELPNLIE
                     LSDLRGDLQMHTNWSDGQRSVEEMARAAKACGYEYILITDHSQSLAVTNGLSPERLKR
                     QRAEIDRVNALNLGIRVLQGSEVEIKADGTLDYPDEILASLDIVLASLHTSLRQERDK
                     VTRRALAAIRSPHVDIFAHPTGRMLYEGGTLEKGREGADLDMEAVLQAAAETGTIVEI
                     NADPQRLDLRDTHARRANELGCLFSINTDAHHPESLAQIHYGVAVARRAWIMAERVVN
                     TWPLKRLRDYLAEGK"
     gene            complement(2517..2675)
                     /locus_tag="AC162G_17845"
     CDS             complement(2517..2675)
                     /locus_tag="AC162G_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HIR5077723.1"
                     /translation="MSTVMLWLPRQEGQGLVEYALILVLVALIVIVILAVLGPAIGNT
                     FSQIVSAI"
     gene            complement(2796..3854)
                     /gene="ygfZ"
                     /locus_tag="AC162G_17850"
     CDS             complement(2796..3854)
                     /gene="ygfZ"
                     /locus_tag="AC162G_17850"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR5077724.1"
                     /translation="MRSTLFDAHVRDGARMAEIDGWEAPDVFTSIEAEYAAAWDAAVV
                     YDSSPVGRLQLTGKTRLDFLDRMSTNDMNALRPGQGAATILTTPIARIVDRVVVYVRE
                     DDAIVLTSRGAQDAVANWLRRYIFFNDDVQVRDAPAELGMLSVYGAKAGEVAARVAGQ
                     DVSDLALHFWRSARDGLIIARADPIASDGFHVLAPDATALTSAWQAAIDAGAAPIGER
                     ALEVLRIESGQPGFGREIGEEYIPLEVGLWPDVSFTKGCYIGQEIIARMESRHRLARQ
                     LVGLRAKAPIQPGTELTANGAAVGRVSSAAVRPGGDSIGLGFVKPAFASPGTRMSIGA
                     DGLTEVEVSRLPLPRAQF"
     gene            complement(3862..4365)
                     /locus_tag="AC162G_17855"
     CDS             complement(3862..4365)
                     /locus_tag="AC162G_17855"
                     /inference="COORDINATES: protein motif:HMM:NF019632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HIR5077725.1"
                     /translation="MTKAELLGEIEREWEALQAAIDGLTDAQMTRMPVAGEWTVKDLL
                     AHIAVCESLLVTDLYKVERGVPPSGVDLTPAQVDRQNAQFYQEQKGRLLERVLEDLYD
                     VHLALVNRLDGFSDVLLSDPKKFKWMKGKPLSAFVAEDSIEHYREHTADILAWRAKLP
                     NRQDARE"
     gene            complement(4362..>4866)
                     /locus_tag="AC162G_17860"
     CDS             complement(4362..>4866)
                     /locus_tag="AC162G_17860"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5077726.1"
                     /translation="PATQQSRDPVLGNLLLAVAAVSWALYTVLARRATQRFSVLAVTM
                     GVTLGGVLIVLPVAAVELQSQTIGAITPAVAAGVLYVGFGSTAVAFYLWNKSFELLDA
                     ATASLFFFAQPVVGVLLSAIFLRERLGLSFFAGAALIALGVLLAMQERQPIVRREANP
                     RSSAEQT"
BASE COUNT          833 a         1671 c         1501 g          861 t
ORIGIN      
        1 ggcaggccct taccctgctc cggcgacagg taagtcgggg tgcccatgcc ggtgccggtt
       61 ccggtcaagt cctcgtcgcg ggcgacgatc ttcgccagcc cgaagtcggc caactgcacc
      121 cactcgcctt cgtccagcag cacgttggac ggcttcacgt cgcgatggat gatgccccgg
      181 ctgtgcgcgt gatccagcgc ggcggcgatc tgctcgacca ggcgcgtcgt ggtgagcaga
      241 tcgatcgggc ggcgcaggcg gtccgccagc gtgccgccgc gcacgtactt catgacgatg
      301 tagctcaggc cgtcttcctg gccgaagtcc atgacgggga gaatgttggg gtggttgagc
      361 tgcgcgatgg ctttcgcttc gcggacgaag cgatcggaga agtcggggac gagcgcgagc
      421 tgctcgggca gcaccttaat ggcgacgtgg cgctcgagcg ccggctgata ggctttgtag
      481 acggcggcca tgccgccctg cccgatcagc tcgacgacgt gatattgccc caggtccctg
      541 ccggcgaggt tcatgatgtc ggggagattc tacatcactt ccgccggcat acaaagttgg
      601 gctctgtgga tcaactattt gccctctgcc agataatccc tcagcctctt cagcggccat
      661 gtattgacga ctcgctccgc catgatccac gcccgccgcg cgaccgccac gccgtagtga
      721 atctgcgcaa gtgattcggg atgatgcgcg tcggtattga tgctgaacag gcagcctagc
      781 tcgttcgcgc gccgggcatg cgtatcgcgc aggtcgagcc gctgcgggtc ggcgttgatc
      841 tcgacgatcg tgccggtctc ggcggcggcc tgcaggaccg cctccatgtc gagatcggcg
      901 ccctcgcggc ctttctccag cgtgccgccc tcgtacaaca tgcggcccgt cgggtgcgcg
      961 aatatatcga cgtgcgggct gcggatcgcg gccagcgcgc ggcgcgtcac tttgtcacgc
     1021 tcctgccgca gcgacgtgtg cagcgaggcc agcacgatgt cgagcgaggc caggatctcg
     1081 tcgggatagt cgagcgtgcc gtcggccttg atctccacct cggacccctg cagcacgcgg
     1141 atcccaagat tcaaggcgtt caccctgtcg atctcggcgc gctgccgctt gagccgctcg
     1201 ggcgacaggc cattcgtcac ggcgagcgac tgcgaatggt cggtgatcag gatgtactcg
     1261 tagccgcacg ctttcgccgc gcgcgccatc tcctcgacgc tgcgctggcc gtcggaccaa
     1321 ttggtgtgca tctgcaagtc accgcgcaaa tcgctcaatt cgatcagatt tggcagctcg
     1381 tcctcaaatt cgccgcgatc ctcccgcagc tccggcggca tgaacggcac gccgagcttc
     1441 gcgtacactt cggcttcttc tctgcagaag aaatccttgc catctttcac acgcgtcagc
     1501 gagtactcgt tgagcgagta acccttcttc agcgcgatct cgcgcaggcg cacgttgtga
     1561 tctttcgagc cggtgaaata ttgcagcgcc gcgccccaat gcctgggctg gatgacacgc
     1621 aaatcgacct gctgcccggt gtgcagccgc acgctggact tcgtcggccc gcgcgcgacg
     1681 acctcggcga cgttgggcag cgcgttgaag gcgtccatga tgtgctccgc gtccgcaggc
     1741 tcggccgaca ccagcaggtc gatgtcgccg atcgtctcgc gcatgcgccg cagcgatccg
     1801 gccaccgacg cgcgctgcac gcctgcgacg gccatcaagc gccgcagcat gtcgcgcgcc
     1861 agcggataag cgatgcccag cggcgagcgg cccgacgcgc cgcgccgccg catcgcctcg
     1921 atgttggcga cgatcttctc ttcgaacttg cggcccagcc cgggcagccc ggcaagctcg
     1981 cccgcgcgcg ccttcttccc cagctcttcg acgctcgtga tgcccgcttc tttccacagc
     2041 agcttggcgc gcttcgggcc caggtccgga atggcgagca tggcgacgac gccagccggg
     2101 atttctttct gcaaccgctc gtacaggctg aacgtccccg tgcggagcag cgagtcgatt
     2161 ttctcctcga tcgcctggcc gacgccgggg atggagcgca gctctccggc gcgccgcagc
     2221 acgttgacgt cccggtcgag attcgcgatc tgctcggcca cgcgccggta ggcgatgatc
     2281 ttgaacttgt tttcctggcc ctcgatgtcg agcatatcgg cgagggtttc gaaggttcga
     2341 gcgacatctt gattgttcat gcggggtcac ctctggatgc ggcaatgggc atacatatta
     2401 tactgctcat acccggaaat ggtgacctgg agacctggca ggtctggtca aagacctgcc
     2461 aggtctgcac aacaaaacag cccccgcgct tgcgagggct gtcccggcag aggacttcag
     2521 atggcgctga caatctggct gaaggtatta ccaatggccg ggccaagtac cgccagaatg
     2581 acgatgacga tcagcgcgac taaaacgagt atcaacgcgt actcgaccag accctgcccc
     2641 tcttgacgcg gaagccacaa catgacggtg ctcacctcct cctcccccag attgtgacca
     2701 gagtcccgtc catcctgtcg ctattctacg agagtaagcg cgtccggtct atgcatcgct
     2761 agtactaaaa tcccagtcgc gcagtaacag cctggttaga attgcgcgcg cgggagcggc
     2821 agccgactga cttcaacctc cgtcaaacca tctgcgccga tggacatacg cgtgccgggg
     2881 ctggcgaacg ccggcttgac gaatcccaaa ccaatcgaat cgccgcccgg ccgcaccgca
     2941 gcgctcgata cgcgacccac cgccgcgccg ttcgccgtta gctccgtacc cggttggatc
     3001 ggcgctttcg cacgaagtcc cacgagctgc ctggcgagcc ggtgccgtga ctccatgcgc
     3061 gcgatgatct cctgcccgat gtagcaaccc ttggtgaagc tgacgtccgg ccacaggcca
     3121 acttccagcg gtatgtattc ttcgccgatc tcgcggccga aacccggctg gccggactcg
     3181 atgcgcagca cttcgagtgc gcgctcgccg atcggcgcag cgccggcgtc gatcgccgcc
     3241 tgccacgccg aggtcaaagc cgtcgcatcg ggcgcgagca cgtggaagcc gtcgctcgcg
     3301 atcgggtccg cgcgggcgat gatgagacca tcgcgcgccg atcgccagaa gtgcagcgcc
     3361 agatcggaca catcctgtcc ggcgactctc gccgccactt cgccggcttt cgcgccgtac
     3421 acggacagca tccccagctc cgccggcgca tcgcgcacct gcacgtcgtc gttgaagaag
     3481 atgtaccggc gcagccagtt ggcgacggcg tcctgcgcgc cgcgtgaggt gagcacgatc
     3541 gcgtcatcct cgcgcacgta cacgacgacg cgatcgacga tgcgggcgat aggcgtcgtc
     3601 aggatcgtcg ccgcgccctg gcccgggcgc agcgcgttca tgtcgtttgt cgacatgcga
     3661 tccagaaagt cgaggcgcgt cttgcccgtc aactgcagcc gccccaccgg cgacgaatcg
     3721 tacacgacgg cggcgtccca ggccgccgcg tattccgcct cgatcgacgt gaagacgtcc
     3781 ggcgcttccc agccatcgat ctccgccatg cgcgcgccgt cccgaacgtg cgcgtcaaac
     3841 aacgtgcttc tcatcatcgt atcattctct cgcgtcttgc cggttcggca gcttcgcgcg
     3901 ccaggccagg atgtcggccg tgtgctcgcg ataatgctcg atagaatcct cggcgacgaa
     3961 cgcgctcagc ggcttgccct tcatccattt gaacttcttc ggatcggaaa gcagcacatc
     4021 agaaaatcca tccaatcggt tcaccaacgc caggtgaaca tcgtacaagt cctcgaggac
     4081 gcgctccagc agtcgccctt tctgctcctg gtagaactgc gcgttctgcc tgtccacctg
     4141 cgccggcgtc aggtcgacgc cggagggtgg gacgccgcgc tcgaccttgt acaggtcggt
     4201 gaccagcagc gactcgcaca cggcgatgtg cgccagcagg tctttgaccg tccactcgcc
     4261 ggcgaccggc atccgggtca tctgcgcgtc ggtcaggccg tcgatggccg cctgcagcgc
     4321 ctcccactcg cgctcgattt cgccaagcag ctcggctttg gtcatgtctg ctccgcggac
     4381 gaccggggat ttgcctctcg ccggacgatc ggctgcctct cctgcatcgc cagcagcaca
     4441 cccagcgcga tcaatgccgc gccagcgaaa aacgacagcc ccaaccgctc gcgcaggaag
     4501 atggcgctga gcaagacgcc gacgacgggc tgcgcgaaga agaacagcga cgccgtcgcc
     4561 gcatccagca gctcgaagct cttgttccac aggtagaacg cgacggcagt cgagccgaag
     4621 ccgacgtaca ggacgccggc ggcaacggcg ggcgtgatcg cgccgatcgt ctgcgattga
     4681 agctcgacgg cggcgaccgg caggacgatg agcacacctc ccagagtgac gcccatcgtc
     4741 acggccagca ccgagaaacg ctgcgtcgcg cgccgggcga gcaccgtgta cagcgcccac
     4801 gacacggccg cgacggccag caggagattg cccagcaccg gatcgcggga ctgctgcgtc
     4861 gccggc
//
LOCUS       DBNHXB010000456        12118 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1489467, whole genome shotgun sequence.
ACCESSION   DBNHXB010000456 DBNHXB010000000
VERSION     DBNHXB010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 12118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12118
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1489467"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="AC162G_17865"
     CDS             complement(<1..397)
                     /locus_tag="AC162G_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077727.1"
                     /translation="MQLRFKVDGTNNGAIYAVVASGSPYRRFGVYAKEGSLVAQYNDG
                     FGWREPADLLSSVQANTWYTLTLLLDDVRGFTAEVAQTTNPAVRGSYNHPMPAGLSWL
                     FQHWVWSGYAYLDDYREFTGNGLAREPDER"
     gene            complement(431..622)
                     /locus_tag="AC162G_17870"
     CDS             complement(431..622)
                     /locus_tag="AC162G_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077728.1"
                     /translation="MTRLADVPRTLSGTLTITLSDAFDAFNAGTWSRGSSANTVIVAD
                     GGNNVLRNAGVNNWGLSTL"
     gene            complement(1051..1848)
                     /locus_tag="AC162G_17875"
     CDS             complement(1051..1848)
                     /locus_tag="AC162G_17875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5077729.1"
                     /translation="MITEVKLWWEKAAENYQQQCRIPIDILYGPGAPNEDELQLIGPV
                     AGKHVLEIGCGGAQAAIAFAKRGAVVTGIDIAEAQLRFARELAAKEGVEITLLQGDMA
                     DLGPIATASQDVVFSACAFGYVDDLFACFAEVHRVLKPTGIFVWSVGHPFNQVLDDHH
                     PEALIVINSYFDTGIHIEGATDTPGEAFASFNRTISDYFDLLIQTGFVVERLIEPDSR
                     KRYACDPWYGLWETTPERCAKLPQTIILKCRKDRVVTLQFITGDAPL"
     gene            complement(2029..5181)
                     /locus_tag="AC162G_17880"
     CDS             complement(2029..5181)
                     /locus_tag="AC162G_17880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077730.1"
                     /translation="MSEKPNTGAGLRCTVAWTMHQHQLEYLIANSRYAGLLNLEARGE
                     QRAHITLTADARRLDLDDATLFGTRASFIETAEQVVWLQHEAQVFCQIGCDAVPGPRF
                     ERPLVPTAEPHVEMDAVPGADRRRFYAVSLTLVDPHTGDRYRHSLMLDPSVAAGDLGV
                     RLLELLLGDAECHATSGYPLAAIAEVVARIGCPIASTIEIVDANNCVRSELGSFQIHE
                     LVLESVSDEVFGIPADYRDVREACRVASADDTARPAFTRLDPVWIDTGWRPSDVDGGL
                     KGTTLRMLSGGYPDREIDVETEQPERPVTTTQAVYGLRIEQRLLDDTRTLVNRALGPF
                     AGATFPLSSTTGLVVDWLPRLRAGVLASRDSNGVPARGLGTFLFSLLHDVEPPALPRT
                     SLLPSVNVARWGTIRGRGLLDRMAKKRAQELVQSSALTPAMLARLPASVVTRLTAAGT
                     NWGGLTPTDRLQIMLAVLLEEFGAPRVSLPGKIEFNWEDLVVGEFTNYTGTLTFPPAA
                     LDAPGICPLITSLRCRYGRIEGSLMLGGITLTGELNRRPGTTYLLMIGASALATLIFP
                     ALWWVVPLLGAIGVFVALDTATLALSSTDVTANLTGRFVRMPNGVFQLRFDVNVTGGM
                     STSLTSNVPTGIHQVVDAVVTAVANSGRILLDRFQAELQSELTKLVRGFLGDGFPASL
                     LRLGVPITGGSIGGRDDQYTYIEAMIAAPANLHIPMIRVPADTEARLSNDLQRLSGRT
                     DARHYLSLVSSQNTINLILAALWRAGAFNARIINSGVRNALKALARPPYPSGANVLRA
                     AVFVVGLPQLSLMQATPIGEHGIVELKALLRLEPHVDDLYEWTVTWRARAQTVIGSVL
                     ASRTPLVQITTALDQPLDLLANLAAATAQVEGLKRIYLIRRTVTEEFTDEFGKPQTIT
                     YQETEENETPFALSAQDAAQHVPLLLEAMRHALAVRDVHRSPRRDGVRANGAFSGTAD
                     SITLQSYILDGTDPDDALPPVASQAPAAYIRGDLGFDSGLLFQHLLLSGKVLSIFDPA
                     SEKDLTRDFAGVILDLLPQE"
     gene            complement(5224..7623)
                     /locus_tag="AC162G_17885"
     CDS             complement(5224..7623)
                     /locus_tag="AC162G_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077731.1"
                     /translation="MAHDTQLLDLYGKLRVELLTRHFAYGRYFRANLISESGTKLGNL
                     PGVANCDTCIAPAEIDDPWKPPKKLRMDNYTTLLALPEPVQMRERWRIGRHDRDNPLL
                     WSGILFTCLAIEHHLASSTARNIIWEGLKSIGSLYKFTEGHFKGYILRWDPVTSDKWI
                     TRVDNDKVVPLQCCEFLTDAARQYLYCTPLDHPAYIPPVDKPPDGLPDDHPQWQAYRA
                     RTAFMPRYRFWEPSMEELAGLILAYDVIHRFLYTDYEIKAEVERQVNMLGDYLAEHGY
                     ILVRPCGGFTARGASGVLPALEFPFTQVFQRITGKTYESRASFDGAMEKAGVWESLKG
                     PLRCGAAIGSTLGVAAALGLVSASLAIGEVLGGITGVLSSIIGVLAGIGVVRLGYAAG
                     RAVALLLNQGIFDVSSDQGEFALADLWKVIPSRKLRFLTWILSTATGLGGHARMFPAY
                     IGLTGLGDPDTTVKDSYITWFSGWPFKYGESITPTHTGLKRGVAAVLGEGPNSERLLR
                     EFLDQFYSVMTTTGAVPISVDPKLNPSERPGRQGHLTVENSPEDENKCREQVLRERPI
                     EGIDFMVGLALAWLHAKRSGDVPNGIPNPPSQKDLDSMPRLEVPFQAYSVLGPIGGAI
                     FPDVPMESEPPPATVFGNNTNASKPPDPPPPTLPPPVRTESTVVDVPESAPGDVFTGI
                     YVDTYDTISIFASGSIESGVWLTGPNGPAGWNNLDHDPKFPINRLSNGVKIRHPKARP
                     YCLLYRIGVGPYQYAGTNANFEVPEGVAKLPLYLRINDDRPGNGKGAFRAVITVIHRA
                     P"
     gene            8143..11238
                     /locus_tag="AC162G_17890"
     CDS             8143..11238
                     /locus_tag="AC162G_17890"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR5077732.1"
                     /translation="MPTLSLTLLGAFQAALDGRPITGFESNKVRALLAYLVVEADRPH
                     SRDELIGLLWPEQPDATARANLRQALANLRQVIGDRTAQSPFLLVTSDSIQFQHPENS
                     GIDVTAFTTLLAECQAHAHRRLVTCRSCARRLQRAVELYRGDFLAQFVQSDSEAFEEW
                     ALVKRERWHHAALEALYALAEYHDRRSEYDREQHYARRQLELDPWREEAHRQVMRALA
                     LSGQRSAALNQYERCRRILASELGVEPARETTRLYEHIRDGSNEDELSASTAVVRLPA
                     SPTPLVGRECEIAEMVELIENPAHRLVTITGAGGIGKTRLAVAAASELAHAFTHGAAF
                     VSLAALSSVEFLAPSILAALDVSLDGQVDAEAQLHAYLRDRELLLIMDNWEHLLPEGG
                     GVELLAEMLQRAPGLTVLATSRERLRIQAEWLFDLQGLDYPKGESATELESYDAIRLF
                     VQRARQAQRRPALDQADLVAIARICQLVEGLPLAINLAAASADVRSCAEIAGEIEASL
                     LGLKSRFRDAPERHRSVWAAFEHSWNLLSERERRVLRQVSVFRGGWQVESAAQVAGAS
                     LETLTALVDKSLLRHDVSGRFDMHELVRQYAGDKLKQAGETEPTRHQHLEFFRELAEA
                     AEMKLEGSEQVQWIDRLKREYGNLEAALQWALDQSVAEVALQMSSALGQFWEIYGNVA
                     EGRQWLRRALALSAPTSDTVRAKALKAAGRLAFEQRDMPAAQALFEAGLELYYGVEAH
                     ADVAALLNNLGNVAWFQGDYPQAHIRYEHALALRRELGDQRGIAGVLNGLGLVAFSQN
                     DLEHAAQYLEQSLSMMEALGDRKGRADAFFNLGLVLLRLESGMGRAIECFEASVALCR
                     ELGYLRTAAYALNNLAMLILHQGNAPRAAALASDSLALCRELDDGMGAFYALINLGHS
                     ALDQGDLGQAVRAFQECRELLGPMDDLSRRECTAWLAEGLAHVDVVRAHDTLAVHLAA
                     AAAMARESNHFVLPPTTRTYLELRLARVRTRLGESAFASAWVEGRAMTPEQAIRIALE
                     N"
     gene            complement(11307..>12118)
                     /gene="yqeB"
                     /locus_tag="AC162G_17895"
     CDS             complement(11307..>12118)
                     /gene="yqeB"
                     /locus_tag="AC162G_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HIR5077733.1"
                     /translation="ALRLHRAGFRITVLEIPQPTVIRRAVAFASAVYDGSTVVEGVTA
                     RLMRLDDDLEPMWRSGEAPVVVDPDASILARLRPDALVDAIMAKRDPSAALRAGLGTH
                     IDGAPMVVALGPGFTAGVDCHAVVETNRGHSLGRVYYAGTAEANTGVPGAVGGQDALR
                     VVRAPVDGVFQGLRKIGDRVSRGDSIGRVGDPSTALRAGFAVASQIDGVLRGILHDGL
                     IVHAGMKVADVDPRGVVEYCFTVSDKAWAVGGGVLEAILHQRSVFTLRKGA"
BASE COUNT         2378 a         3609 c         3715 g         2416 t
ORIGIN      
        1 gccgctcgtc cggctcgcgc gccagcccat tgcccgtgaa ctcgcggtag tcgtccaggt
       61 aggcataccc gctccacacc cagtgctgga acagccagct caagccggcc ggcatcgggt
      121 ggttgtagct accacgcacc gccgggttgg tggtctgcgc cacttcggcg gtgaagcccc
      181 gcacgtcgtc cagcagcagc gtgagcgtgt accaggtgtt cgcctgcacg ctgctcagca
      241 ggtcggccgg ctcgcgccag ccgaagccgt cgttgtattg cgccaccaag cttccttctt
      301 tcgcatacac accgaagcgc cgatacgggc tgccgctcgc caccacggcg tagatcgcgc
      361 cgttgttcgt gccgtccacc ttgaagcgca actgcacgcc ggtgttgtgg ctcaggctgt
      421 actgattgcg tcacagagta gacagccccc agttgttcac gcccgcgttg cgcaggacgt
      481 tgttgccgcc atctgccacg atgacggtat tggcgctcga cccgcgcgac cacgtcccgg
      541 cgttgaacgc gtcaaacgcg tccgacagcg tgatagtcag cgtcccgctg agcgtgcgcg
      601 gcacatccgc caggcgcgtc acgttgccga ctgcgtcgta gccgatcgtc gtcgtgaagc
      661 cgcgcgacag acgttggaag aaatttgcgc cgggtttagc gaagggtttg cgaccgccga
      721 tttgaaagag gccagcgcct tgcttgataa tgagacgact gcccgatcat tagcggaata
      781 gcgccagagc aaattgcatc actaaattaa gaatccaacc cgagtcctca tggatggtcg
      841 ataaagccca ccatgaggtc tccggttcgc gttatgcaaa gagtggttcg ggtccgtgag
      901 gtcccgggtt caagtcccgg caccccgact gggacgacga ttcatgggca aacagcccac
      961 ctgatcgagg cgggctgttt cgtttagcgt ggcgatgcca gacgcacgct ctgggtatcc
     1021 agcattcgtg cagccggatg cggacgcgaa ttacaaggga gcgtcgcccg tgatgaactg
     1081 gagtgtgacg acccgatcct tgcgacactt gaggatgatg gtctgaggta gtttcgcgca
     1141 ccgctccggg gtagtttccc acagaccata ccagggatca cacgcatagc gtttgcggct
     1201 atctggctct atcagccgtt cgaccacgaa tccggtctgg atgagcagat cgaagtaatc
     1261 tgatatggtc cgattgaatg aggcaaaagc ctcgcccggc gtgtctgttg ctccttcgat
     1321 atgaattccg gtatcgaagt aagaattaat cacgatcaga gcctcaggat gatggtcatc
     1381 caagacttga ttaaaagggt gccccacgct ccacacgaaa ataccagtcg gttttagtac
     1441 gcggtgaacc tcggcgaagc aagcgaaaag atcgtcaaca tagccaaagg cgcacgcaga
     1501 gaagaccaca tcctgactgg ccgtggcaat tggcccaagg tcggccatgt cgccctgtaa
     1561 aagcgtgatc tccaccccct ccttcgctgc cagctctctc gcaaagcgga gttgcgcctc
     1621 cgcgatgtcg attcctgtca ccacggcgcc gcgtttggcg aaggcgatgg ccgcctgcgc
     1681 gccgccacat ccgatctcca gaacgtgttt cccggcgacc ggcccaatga gttgaagttc
     1741 gtcttcgttg ggcgcgcccg ggccgtagag gatatcaatg gggatgcgac actgttgctg
     1801 gtaattctcg gctgcctttt cccaccatag tttgacttcg gtgatcatgt tttctccctt
     1861 gtgcaacacc gcgcatcagc cgctggcgtg ctgcgcccgg cccgctgaca agatctaccg
     1921 tgccgccgat tctggcatca aggccgacaa atctgctggc tgctgcaaac cccttatctt
     1981 tgtgggcagt aacgatctca tccaatcgca agttgctggc gataagtgtt actcttgagg
     2041 aagaaggtct aggatgacgc ccgcaaagtc gcgcgtgaga tctttttcgg acgcggggtc
     2101 aaatatggaa agcaccttcc cggagagcag gagatgctga aaaagcagtc cgctgtcaaa
     2161 accgagatcg cctctgatat atgccgccgg tgcctgtgac gcgacaggcg gcagcgcatc
     2221 gtcgggatcg gtgccgtcga ggatgtatga ctgcaaggtg atggagtcgg cggtcccgct
     2281 gaatgcgccg ttggcgcgga caccgtctcg ccgtggcgaa cggtggacat cacgcactgc
     2341 gagggcatgc cgcatcgcct ccagaagcaa cgggacatgt tgagcggcgt cctgcgcaga
     2401 tagtgcgaac ggcgtttcgt tttcttcggt ttcctggtat gttatcgttt gtggcttccc
     2461 aaactcgtct gtaaactcct ctgtaaccgt acgacggatc aagtagatgc gcttcagtcc
     2521 ttccacctgg gcagtcgcgg ccgcgagatt ggccagcaga tcaagcggct ggtcaagtgc
     2581 ggtggttatc tgcacgagcg gcgtgcggga tgcgagcact gagccgatga ccgtctgtgc
     2641 tctggcgcgc catgtcacag tccattcata aagatcatcg acgtggggct cgagtcgtaa
     2701 taaggccttg agctccacga tgccgtgctc gccgatcgga gtggcttgca tgagcgagag
     2761 ctgtggtagg cccacgacga agactgcagc cctgagcacg ttcgcgccgg aagggtatgg
     2821 cggccttgcg agggccttga gcgcattgcg cacacccgag ttgataatcc gcgcgttgaa
     2881 agcgccggcg cgccaaagag cggcgagaat caggttgatc gtgttctgtg agctcaccaa
     2941 tgaaagataa tggcgcgcat cggttcttcc gctgaggcgt tgcaagtcgt tggacagccg
     3001 cgcctcggta tcagccggaa ccctgatcat cgggatgtgc aggtttgccg gggcagcaat
     3061 catcgcttcg atgtaggtat attggtcgtc acgtccgccg atgctgccgc cggttatcgg
     3121 gacgccgaga cgcaacagcg aggcggggaa cccgtcgccc agaaagcctc tgaccagctt
     3181 cgttagctcg ctttggagct ccgcctggaa gcggtctagc aagatccttc ctgaattggc
     3241 gacggccgtg accactgcgt caaccacctg atgaatgccc gtcgggacgt ttgaggtaag
     3301 gctcgttgac atcccacctg taacgttaac gtcgaagcgc agctgaaata caccgttcgg
     3361 catgcggaca aagcggccgg tgaggtttgc ggtgacatct gtgctcgaga gggcaagggt
     3421 tgctgtgtcg agggcgacga acaccccgat cgcgccgagt agcggaacca cccaccacag
     3481 cgcagggaaa atgagcgttg ccagagcgct ggcaccgatc ataagaagat aggtagtgcc
     3541 cggacgccga ttgagttcgc cagttagagt aatcccgcca agcatgagac tcccttcgat
     3601 cctgccatag cggcagcgaa ggctcgtgat aagcggacaa atgccgggcg catccagcgc
     3661 ggccggggga aacgtgagtg tgcccgtgta gtttgtgaac tcgccgacca ccaaatcttc
     3721 ccagttgaac tcgatcttac ctggcagcga cacccgaggc gcaccaaact cctctaacag
     3781 cacggcaagc atgatctgga gtcgatcggt aggagttagg ccgccccagt tcgttccggc
     3841 tgcggtaagc cttgtcacaa cactcgctgg taggcgagcg agcattgccg gtgtcagcgc
     3901 gctgctctga acgagctctt gagcgcgctt cttggccatt cggtcaagca gcccacggcc
     3961 gcgaatggtt ccccagcgcg ctacgttcac cgacggcagc agggaagtgc gcggcagcgc
     4021 tggcggctcg acatcgtgca gcaggctgaa caagaaagtt ccaaggcccc gcgctgggac
     4081 gccattgctg tcgcgtgagg cgagcacacc tgcccgtagc ctggggagcc agtccaccac
     4141 aagcccagtc gttgagctga gcggaaaggt agcgccggcg aatggaccaa gcgctcggtt
     4201 gaccagggtg cgcgtgtcgt cgagcaaacg ctgctcgatc ctcagaccgt acacggcctg
     4261 cgttgtggtc acgggtcgct caggctgctc ggtctccaca tcaatctcac ggtccgggta
     4321 cccgccagaa agcatgcgca gggtcgtacc tttgagaccg ccatctacat cgctcgggcg
     4381 ccagccagtg tcgatccaca ccgggtcgag gcgcgtgaag gccggtctcg cagtgtcatc
     4441 agccgaggcg acgcggcagg cctcacgcac gtcgcggtag tctgcaggga tgccaaagac
     4501 ctcgtccgac acgctctcca gaacgagttc gtggatttgg aagctaccca gttcgctgcg
     4561 tacacaattg ttagcgtcga cgatctcgat ggtgctcgcg atcgggcacc cgattcgtgc
     4621 aaccacctct gcgattgcag caagcggata tccgctcgtc gcgtggcact cggcgtcacc
     4681 gagtagcaat tcgagtaatc gcacaccaag atccccggcc gcgacgcttg gatcgagcat
     4741 caagctatgg cggtatcggt cgcccgtgtg agggtcaacg agggtgagac tgacggcgta
     4801 gaatcttcgc ctgtcggcac cgggcacggc gtccatttcg acatgcggtt ccgcggttgg
     4861 aacgagcggt cgttcgaaac gtggtcctgg tacggcgtcg cacccgattt gacaaaatac
     4921 ctgcgcctcg tgctgcagcc aaacgacctg ctcggcagtc tcgatgaaac tcgcccgcgt
     4981 gccaaagagc gttgcatcgt cgaggtcgag gcgacgcgca tctgcggtga gcgtgatgtg
     5041 ggcacgctgc tcaccacggg cctccagatt tagcaggcca gcatatcggc tgttggcaat
     5101 aagatactcg agttgatgct ggtgcatcgt ccaggccacc gtgcaccgga ggcccgcacc
     5161 tgtgtttggc ttctcactca taggtcctcc taaactctgc gctcgccgcg ctgcagtgcg
     5221 cggctacggt gcgcggtgaa taaccgtgat cactgctctg aacgcgccct ttccgttgcc
     5281 tggcctgtca tcgttgatac gtagatagag cggtaatttg gccacccctt cgggtacttc
     5341 aaaatttgcg ttcgtaccag catattgata aggtcctacc ccgatgcgat agagcagaca
     5401 atacggccgc gccttggggt gtctgatctt gacgccgttt gagagccggt taatagggaa
     5461 cttcggatca tggtcgaggt tgttccagcc tgccggaccg ttcggccctg tgagccacac
     5521 acctgactcg atgcttccgg aggcgaatat cgagatggtg tcgtaggtgt ccacatagat
     5581 gccagtgaac acgtcgccgg gcgcgctttc gggcacatcc actactgtcg attccgttct
     5641 gaccggcggc ggaagggttg gcgggggtgg gtcaggcggc ttcgaggcat tcgtgttatt
     5701 accaaacacc gtcgctggcg ggggctcact ttccatcggc acgtcaggga atatcgcccc
     5761 gcctatcggc ccgagcacgc tgtacgcctg aaatggcact tcgagcctcg gcatgctatc
     5821 gagatccttc tgcgacgggg gattcgggat gccgttcggc acatccccgc tgcgtttggc
     5881 atgcagccag gcgagggcaa ggccaaccat gaagtcgatc ccctctatcg gcctttcccg
     5941 aagcacctgc tcacggcatt tgttttcgtc ttcggggctg ttttcaactg tcaaatgacc
     6001 ctggcggcct ggacgctccg atgggttcaa cttgggatcc accgatatgg gtaccgctcc
     6061 cgtcgtcgtc atcacggaat agaactgatc gaggaactcc cgcaggaggc gttccgagtt
     6121 cggcccttcg cccagcactg cggcgacacc gcgctttagg cccgtgtggg ttggggtgat
     6181 actctcaccg tacttgaatg gccacccaga gaaccaggta atgtagctat ctttcaccgt
     6241 cgtgtcagga tcgcccagcc ccgtcagacc aatgtatgcc gggaacattc tggcgtgacc
     6301 gccaagtcca gtggccgtgg atagaatcca agtcaagaaa cgtagtttgc gggacgggat
     6361 cactttccac aagtcggcaa gtgcaaattc accctggtcg gacgatacat cgaatatgcc
     6421 ctgattaagc agcaaggcga cggcccggcc ggccgcatag cccaggcgta ccactccaat
     6481 gcctgcgagc actcctatga tgctgctaag cactccagtg ataccgccaa gcacttctcc
     6541 aatggccagc gatgcggaca cgagccccaa ggcagccgcc acgccgaggg tcgagccgat
     6601 cgcggctccg cacctgagcg gacctttcaa tgactcccaa acccccgctt tctccatcgc
     6661 gccatcgaaa cttgcccgcg attcgtaggt tttgcctgtg atgcgctgga atacctgcgt
     6721 gaaagggaat tccaaagccg gcaatacgcc ggatgcgccg cgcgctgtga agcctccgca
     6781 gggtcgcacg aggatgtagc cgtgctcggc gaggtagtct cctaacatgt tgacctgccg
     6841 ctcgacctcg gccttgatct catagtcggt atacagaaac cggtggatga catcgtacgc
     6901 caagatcaat ccggcaagct cctccatcga cggctcccag aagcgatagc gcggcatgaa
     6961 cgccgtcctt gcgcggtatg cctgccactg cgggtggtcg tcgggcagtc cgtccggagg
     7021 cttgtccacg ggcgggatgt aagcaggatg atcgaggggc gtgcagtaaa gatactgccg
     7081 cgctgcatcg gtcaagaact cgcaacattg gagtggaacc accttatcgt tgtcgacccg
     7141 cgtgatccat ttatccgatg taaccggatc ccagcgcagg atataccctt tgaagtgtcc
     7201 ttcggtgaat ttgtataacg aaccgatgga ttttagacct tcccagatga tgttgcgcgc
     7261 ggtcgatgat gccagatgat gttcaatggc caggcatgta aacagtatcc ctgaccatag
     7321 caatgggttg tcgcgatcat gacgtccgat gcgccaccgt tcacgcatct ggaccggctc
     7381 ggggagcgcc aggagcgtag tgtaattgtc catgcggagt ttttttgggg gcttccaggg
     7441 atcatcaatc tcagccggag cgatgcaggt gtcgcagttg gcaacgcccg gaaggttgcc
     7501 gagcttcgtt ccggactcgg agatgaggtt ggcgcgaaaa tatcggccat acgcgaagtg
     7561 tcgggtgagc aactcgaccc gcagtttgcc gtatagatca agaagttgag tgtcgtgtgc
     7621 catcacaaac tccttccaac cggcctgcac aagccgagca accctgtccg cttcatggca
     7681 aatctcctct cttttcgatc ggcgtagaac gtgccttgcg tgatgcgtcc tctagctgat
     7741 gcttgagaaa ggcgcacagg aactgcgcat ccgcaaaccc tcgccgctca cccgtgtgcg
     7801 catcctccag tgatgcgcgc catacaacac tatcttcttc gatgacacac cacagacgga
     7861 gcaggaacgc catatagcat ggaggctgat cgttcatgag ctagttcatt gtggcattaa
     7921 acataatccc ctggctgatg caacaggcgg ctggctctat ctacatcagc atagcacacg
     7981 agcgtaagga aaacgcaagg tagacagcgc gcgccgacag gtgtatcatg gaattcgacg
     8041 atttacgttt tgctcccctg ctaaaacgcg ctggataagc gtccgcacag tacattgaac
     8101 acagctcaga tcgggcggca ggtcgccaga ggaggtaaca cgatgcccac tctgtctctg
     8161 actctacttg gcgcttttca agccgcgctc gacggccgcc ccatcaccgg cttcgagtcc
     8221 aacaaggtgc gcgccttgct ggcttatctt gtggttgagg ccgatcgacc tcattcgcgc
     8281 gacgagctaa tcggcctgct gtggcccgag cagcctgacg ccaccgcccg cgccaatttg
     8341 cgtcaggccc tggcaaatct gcgacaagtc atcggcgatc gcacggccca gtcgcccttt
     8401 ctcctcgtca cgtcagacag cattcaattc caacatcctg aaaacagtgg catagatgtc
     8461 acggccttca caacgctcct ggctgaatgt caggcgcacg cgcatcgccg cctcgtcacc
     8521 tgccgatcgt gtgcgcgacg attgcagcgg gctgtcgagt tgtatcgcgg cgatttcctg
     8581 gcgcagttcg ttcagagtga cagtgaggcg tttgaagaat gggcgttagt gaagcgcgag
     8641 cgctggcatc atgccgcgtt ggaagcgctg tatgctttgg cagagtatca cgatcgacgg
     8701 agcgaatacg atcgagagca gcactatgcc cggcgtcaac tggaacttga tccctggcgt
     8761 gaagaagcgc atcgacaggt gatgcgcgcg ctggccttaa gcgggcaacg cagcgcggcg
     8821 ctcaatcagt atgagcggtg tcgtcgcatc ctcgccagtg agctgggcgt cgaaccggcg
     8881 cgtgaaacca cccggctgta cgagcacatt cgggatggct caaacgaaga cgaactttct
     8941 gcatcaacgg cagtggttcg tttgcccgca tcacccacgc cgttggtggg acgcgagtgc
     9001 gagatcgccg agatggtcga actcatcgag aatccggcgc atcgcctggt gacgatcacc
     9061 ggcgcgggcg ggatcggcaa aacgcgattg gccgtggccg ccgcaagcga actggcccat
     9121 gcctttacgc atggcgcagc gtttgtctcg ctggcggcgt tgagctcggt cgagtttctc
     9181 gccccgtcga tcctcgccgc gctggacgtt tcgcttgacg gccaggtgga tgccgaggct
     9241 caactgcatg cctatctgcg tgatcgggaa ctgttgttga tcatggacaa ctgggagcat
     9301 ctgcttcccg aaggcggcgg agtcgaactg ctggccgaga tgctccagcg cgcgcccggc
     9361 ttgacggtgt tggcaacctc gcgtgagcga ctgcgaatac aggccgaatg gttgtttgat
     9421 ctgcaaggtc tggattatcc gaagggcgaa tcggccacag aactcgaatc ctatgacgcc
     9481 atccggttgt ttgtccagcg cgcccgccaa gcccaacggc gcccggcgct cgatcaggct
     9541 gaccttgtgg ccatcgcgcg catctgccag ttagtggaag gcttgccgct cgcgattaat
     9601 ctggccgccg cttcggcgga tgtgcgttcg tgcgccgaga tcgccggcga gatcgaggcc
     9661 agcctcctcg ggttgaagag tcgctttcgg gatgcgccgg agcgccatcg cagcgtgtgg
     9721 gctgcgttcg agcattcgtg gaacctgttg tccgaacgag agcgccgcgt gctccggcaa
     9781 gtctcggtgt ttcgaggcgg atggcaggtc gagtcggccg cgcaggtggc cggagcctca
     9841 ctcgaaacgc tgactgcgct cgtggataaa tcgctgttgc ggcacgatgt gtcggggcgc
     9901 tttgacatgc acgaactcgt gcgacagtat gcgggcgaca agttgaagca ggctggcgag
     9961 acagagccga cacggcatca gcacctggaa ttctttcgtg agttggctga ggcggctgag
    10021 atgaaacttg agggatcgga gcaggtccaa tggatagatc ggctgaagcg cgaatatggc
    10081 aatctggagg cggcgctaca gtgggcgctg gatcagtccg tggccgaagt ggctttgcag
    10141 atgagcagtg cgctcggtca gttctgggag atatacggta acgtagccga agggcgccag
    10201 tggctgcggc gagctctggc tctcagtgcg ccgacctctg atacggtacg ggcgaaagct
    10261 ttgaaggcag ccgggcgact ggcgtttgag cagcgtgaca tgccagcggc ccaggcactt
    10321 ttcgaggctg gcttggagct gtattatgga gtggaggccc acgcggacgt cgcggcacta
    10381 cttaacaacc tgggaaacgt cgcatggttt cagggagatt acccgcaagc gcacatccgc
    10441 tacgagcatg cgctggcgct gcgccgagaa ctgggtgacc agcgcggcat cgcgggtgtg
    10501 ctcaacggcc tgggcctggt ggcgttcagt cagaacgatc tcgagcacgc ggcgcaatat
    10561 ctagagcaaa gcctgtcgat gatggaggcg ctgggtgacc gaaagggtag ggccgatgca
    10621 ttcttcaatc tcgggttggt cttgctgcgc cttgagagtg gcatggggcg agccatcgag
    10681 tgttttgaag ccagcgtggc gctttgtcgt gaactaggct atctgcggac ggccgcctat
    10741 gcgctcaaca acctcgcgat gctgattctg caccagggta acgcaccgcg agccgcggca
    10801 ctggcttcgg acagcctggc gctgtgccga gaattggatg atggcatggg tgctttctac
    10861 gcgctgatca acctgggaca tagtgcgctc gatcagggcg atctcggaca ggcagtccgc
    10921 gcatttcagg aatgccgcga attgctcggc cctatggacg atctttcacg tcgcgagtgc
    10981 acggcttggc tggcagaggg attggctcat gtcgatgtcg tgcgggcgca cgatactcta
    11041 gctgtacatc ttgcggcggc tgctgctatg gctcgcgagt cgaatcattt cgttctaccc
    11101 cctacgaccc ggacgtatct ggagctgagg ctggctcgag tgcgaactcg gcttggcgag
    11161 tccgccttcg cgtccgcctg ggttgagggc cgcgcgatga caccggagca ggctatccgc
    11221 attgcgctgg agaactaggc tttgacgtca cttgctcaca ccgttctgtt cgaaccgagg
    11281 caaacgacgc ctcggctggt aaaggcttac gctcccttcc gtaaggtaaa gaccgagcgc
    11341 tgatgcagga tcgcctccag cacccctccc ccaactgccc acgccttgtc cgaaacggtg
    11401 aagcagtact ccaccacccc gcgcggatcg acatccgcga ctttcatgcc cgcatgaacg
    11461 atcagcccgt cgtgtaggat gccgcgcagc acgccgtcga tctgcgacgc gaccgcgaag
    11521 cctgccctga gcgcagtcga agggtcccct accctgccga tcgagtcacc cctggatacg
    11581 cggtcgccga tttttctaag cccctgaaac acgccgtcca ctggcgcgcg cacgacgcgc
    11641 agcgcatcct gcccgcccac ggcgccgggc acaccggtgt tcgcctctgc cgtcccggca
    11701 tagtagacgc gcccgagaga gtgccctcga ttcgtctcga cgacggcgtg acaatcgacg
    11761 ccggcggtga agcccggccc gagcgcgacg accatcggcg cgccgtcgat gtgcgtgccg
    11821 aggcctgccc tgagcgcagc cgaagggtca cgcttcgcca taatcgcatc gacgagcgcg
    11881 tcaggccgaa gccgcgccag gatcgacgca tcgggatcga cgacgaccgg cgcttcgccg
    11941 gagcgccaca tcggttcgag atcatcgtcc agtcgcatga gccgggcggt gactccttcg
    12001 acgaccgtcg atccatcgta caccgccgat gcgaaggcga cggctctgcg gatcacggtc
    12061 ggctgaggaa tttcgagcac ggtgattcgg aagccggcgc gatgcaggcg cagcgcga
//
LOCUS       DBNHXB010000457        11427 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2327880, whole genome shotgun sequence.
ACCESSION   DBNHXB010000457 DBNHXB010000000
VERSION     DBNHXB010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11427)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2327880"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..676
                     /locus_tag="AC162G_17900"
     CDS             254..676
                     /locus_tag="AC162G_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077734.1"
                     /translation="MTEERQTQAVFEGLVFDEAGHPLEVVLLGGEPHYVIDDGGFKRH
                     VEAAYIDRQVLGAMQAQIDENKDAVEESMLRLIGQDDLFTKAAVDSSLSHVDWLMQRG
                     IPLEARQWLGMMGFRIVVDVHGDVVRIEGGGIVDEGDQ"
     gene            851..1207
                     /locus_tag="AC162G_17905"
     CDS             851..1207
                     /locus_tag="AC162G_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR5077735.1"
                     /translation="MVEPFGFIGIFIAVAFILASVPVLLNWLVAPKKPNPIKDETYEC
                     GIETVGDTWVQFKAQYYIYALIFVVFDIEAAFLLPWAVAYKQVPLVGVIAMLLFIVLL
                     ADGLVYAWRKGDLKWR"
     gene            1286..2503
                     /gene="nuoH"
                     /locus_tag="AC162G_17910"
     CDS             1286..2503
                     /gene="nuoH"
                     /locus_tag="AC162G_17910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HIR5077736.1"
                     /translation="MDPFSSIAVALENLLASLGFADWLIDLIMAVLRSVILGTVALLA
                     FMFLTWLERKLIGRIQDRLGPTYAGPIGILQPIADGIKTLTKEIVVPKGADRWIYFSG
                     PILAAMAALAVFAVIPFGPDTGNGPLVGTALNVGLVYVLAVGAAGIVATIMAGWGSNN
                     KFAVLGTFRAVAQLISYEVPQLLSLVAVAMVAGTMDMVKIVEGQTVPFILPLFVTALL
                     FLISGVAESGRSPFDLLEADSEIVAGFHIEYSAMMFALVQMAEYIHAFAISCIVVTVF
                     LNGWAWPFLPGLAPIWFLVKCFAVFFILIWFRGTLPRFRIDQMMAFNWQFMVPLALVN
                     VMVVAVVDRTLSDAAGWTSASQPWGWGLVMFAANVVMLLAALGLMSLKGREARMRDAE
                     KARSRSAVAATGR"
     gene            2567..3085
                     /locus_tag="AC162G_17915"
     CDS             2567..3085
                     /locus_tag="AC162G_17915"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J family
                     protein"
                     /protein_id="HIR5077737.1"
                     /translation="MTAQQGFFVFFSLVTLIAGLRVVTARNLFHAAIWLVLALAGVAA
                     LFAVLDAGLLAAVQVLIYIGAIAILIIFAIMLTRGMMAPERRRLNSWSATGAVVSLVL
                     LIVLAQAIIAFPWVTPGAGVPEPPADSIETLGEALVDPNQFVLPFELASVLLLVALVG
                     GIFVARERRGNE"
     gene            3166..3474
                     /gene="nuoK"
                     /locus_tag="AC162G_17920"
     CDS             3166..3474
                     /gene="nuoK"
                     /locus_tag="AC162G_17920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR5077738.1"
                     /translation="MVPLQGYLIVGAALFCIGVFGVLARRNAIAILMGIELMLNAVNI
                     NLVAFWRYVEPTAATGQVFALFVLTVAAAEAAVGLALIIAIYRHRASVNVEEINLLKW
                     "
     gene            3607..5841
                     /locus_tag="AC162G_17925"
     CDS             3607..5841
                     /locus_tag="AC162G_17925"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit 5 family
                     protein"
                     /protein_id="HIR5077739.1"
                     /translation="MSPETLALITLLIPIPPLLAFFAIMLFTRKYHAASHTIAIGAAL
                     VSFALALIILVNVVPGGSAELADEPIHLSFPWLPTGATTLDVGVMVDPLTAVMLAMVP
                     LVCLLIIFYSVGYSNFNKKPDEHDHPGQPPHHGREPLYSRFFAFLSLFTAAMLTLVVA
                     DDLLLLFIGWEVMGLCSYLLIGFWYARNYDDPNQITPRQAAVKAFMTTRIADVIMLIG
                     IVALYAATGTLSFREIFTEETTHALATTLFLGIPLAQLTFLLLFIGTIGKSAQFPLHV
                     WLPDAMEGPTPVSAMIHAAAMVSAGVYMIIRMFPLLAGTAEHGHVPAFDAMTWIGAFT
                     ALMAATMALGQNDVKRVLAYSTISQLGYMVAALGIGAYVAAAFHLIMHAFFKALLFLG
                     SGSIIHGVEHGMHVAHETADVTHHADQFLGSGGAHGGPEKIDPQNMMFMGGLRKKMPV
                     TFWTFLIGGLSLAGFPFVTSGFWSKDEILGDALLHAPPVFWVLATAAFLTAFYTMRQI
                     SLTFLGKPRTAAAEHAHESHWTMILPLVILAVLSLAAGFVGVKGDMPFLGPIFRSGEP
                     GTAVEEWLAPTIENVIALEAPEFSWTPVAASIVIALGGLAAGYWVYARRPLAAGQPDP
                     LERLGGVYTFLKHKWYFDELYGRVFVRPSIALAAAVYKWIDRWLIDGVLHAIARGMWR
                     LAGSFRAFDRLVINGGVDWSVDQLKVFGRWFRQTQTGWVQQYLYVVVIGVLVLAALYL
                     ALSL"
     gene            5914..7467
                     /locus_tag="AC162G_17930"
     CDS             5914..7467
                     /locus_tag="AC162G_17930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 4 family protein"
                     /protein_id="HIR5077740.1"
                     /translation="MDLINANILPLVTFTPLVCAVIVMLIPRAVGDRTVKWIVFGLSL
                     VPAALAIYLWLVYPSAPADASGFKFVVDLPWFPQINSRFILGVDGISVSMILLTGILT
                     PLAILISFSIHERVKQYMALFLMLEMGLLGVFVALDMIIFFIFWEIGLVPMYFLINQS
                     GSGNRDYASFKFFIYTMAGSIGMLLSFQVIWMALGAQGGGTYSIVEIAQRWPTFDGTL
                     FGLPASTVKSITFWLFTIAFAIKVPVWPFHTWLPDAHTEAPTAGSMLLAAVLLKLGAF
                     GFLRIVLPFFPAESAQFAPALAILALLGIVLAAFSAFGQDDFKRLVALSSVNHMGFVV
                     LGIAVAAYALGSSDPAVKENAVIATNGAVLQMWAHGLSSAAMFALVGVVYDRAHTRDL
                     KQLGGLWAVMPIYGGVLVFSAMANLGLPGLAGFPAEFSVVRGAWPVFSLITALSTIGL
                     LFTGAFILKAIGKVLHGPESEAAHHVSEMNLTERIALAPLLVLMLATGLFPAWVLNVV
                     NETVARLIG"
     gene            7547..9064
                     /locus_tag="AC162G_17935"
     CDS             7547..9064
                     /locus_tag="AC162G_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 4 family protein"
                     /protein_id="HIR5077741.1"
                     /translation="MTFPFLTVITFTPIVAAVILLLLPPDKKTLIKTIALIATAISLA
                     LSIFVFATYDQQAGATDPARQFQFQEQVQWVPSLGISYHVGADGINLPMILLTGIVAF
                     GGVMISWGIEDRTREFFTFFMLLVAGVFGVFVSLDLFLLLFFYELSIFPMYILIATWG
                     STRKEYAAMKLTLYLLIGSIVALVGVLAIYFSATTPGGQRSFDLLVLRESSIPIEVAR
                     FWFLPIFLGFGVLAGIWPFHSWSPDGHVAAPTAVSMIHAGVLMKLGAYSALRVGVEVL
                     PQAAREMLPIIILLVTINVVYGASIAMVQRDFKYVIGFSSVSHMGLVTMGFATLNQTG
                     LNGAVMQMFSHGVMTALFFSVVGLVYDRAHTRDIPSLGGMVRVLPIAAVAFIIGGLVS
                     MGMPGLSGFVAELQVFMGVWEGNVVAVWYPWVAIIGALGIILTAAYVLRVVGLVFFGK
                     YDEHKWHDMRPANVLDKTALVMFCATMIVVGVLPTLMMNMIQAGTTPFIARLLGG"
     gene            9068..10555
                     /locus_tag="AC162G_17940"
     CDS             9068..10555
                     /locus_tag="AC162G_17940"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR5077742.1"
                     /translation="MDLSQLNTGLLAVLPEILLLALGFVVIGADLFVGAHRKRALGGF
                     TTLGMLVVLIVIGVQSAPLVAGAQAQPTLGNMVVGDLTTQLFRGMFVIAGALTCLISM
                     DFKSLKQAGEYYGLVTFAVLGMCLMAAASDIIMLYVALETTSISLYLLAGYLRGVPLS
                     SEAGMKYFLFGAFTSTILLYGLSLLYGFTGQTGYGALVDALSSLGPESVLPTVVVVIL
                     ILVGFGFKVAAVPFHMWTPDVYEGAPTPITAFISVASKAAGFAVLLRVFLTVFTPLQD
                     QWVTLISILAVLTMTLGNLLAIPQRNIKRLLAYSSIAQAGYVLIGVASARSGANAVFG
                     PASALFYLGVYVLTNIAAFVVVEVVTNETGTENLYEMAGLSKRSFWLALGLMMAMLSL
                     GGVPPLAGFFAKFYVFQAAVGSGLVWLAIVGVLNAIVGLYYYLIVIRVIFVDPGQGEL
                     TAFRIPLTLRTGLFVTTAGILILGVLVTPWYNLVTQAATTLVSGS"
     gene            10653..10946
                     /locus_tag="AC162G_17945"
     CDS             10653..10946
                     /locus_tag="AC162G_17945"
                     /inference="COORDINATES: protein motif:HMM:NF021073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR5077743.1"
                     /translation="MNEREPEPAPLATISKGLVQGSAIGVQAGCLGAGLVIGALVLGL
                     WLDGQLGTRPWLTLGLLFLSMPISVYAIYRVALRAARSMQRRERTSKEDGPLP"
     gene            10943..>11427
                     /locus_tag="AC162G_17950"
     CDS             10943..>11427
                     /locus_tag="AC162G_17950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR5077744.1"
                     /translation="MTRFRKLITRVVIIAVVMFLCGCTALFNLPGLPQLFPVVLPTIS
                     VEPEPVACIGGGLPHCEGGFPITNSLVATLIVDVLLIAVFFSAARRPKLVPSGVQNFL
                     ESLIEILYNQAEQVAGRNARMIFPVGATIFLFVFFANYMELLPGVDSVGYVHRVEEEG
                     V"
BASE COUNT         1763 a         3571 c         3632 g         2461 t
ORIGIN      
        1 ctggctccca atcctggaag acatagcttt gactcatccg cgccccaaca tctatcgtgc
       61 ccctggccgt ctaccatgtc caagctgcca gagtatagtg gatgggtcgc gggcggaggt
      121 ccgtttgaaa gtttacatcg gaatcgaccg gagtcaagcc caacaggacg cattggttcc
      181 tgccggttga tcaaatccgc gtttcgggga ataatccacg gtgccgatgc atcgcacatc
      241 gcgcggggga atcgtgacag aagaacgcca aacgcaagcc gtatttgaag ggctggtatt
      301 cgacgaagcc ggccatccgc ttgaggtggt attgcttggc ggcgagccgc attacgtgat
      361 cgacgatggc ggtttcaagc gccacgtcga ggccgcctac atcgatcgcc aggtgttggg
      421 cgccatgcag gcgcagatcg acgagaacaa agacgcagtc gaggagagca tgctgcgcct
      481 gatcgggcag gacgatcttt tcaccaaggc agccgtcgac tcatcgctga gtcacgtcga
      541 ttggctcatg cagcgcggca taccgctcga agcccggcag tggctgggca tgatgggctt
      601 ccgcatcgtc gtcgacgttc acggcgacgt ggtgaggatt gagggcggcg gcatcgtcga
      661 cgagggcgat cagtaatatt tttcgttttt accgttgccg gttgacttct tgtgtgttat
      721 aatctcggac gctttgtgac ctttgcaaca aagacacccc cgcccgacga cttcaagctg
      781 atcacagccg cgtgccggtt cggcggccgt cgaagcgacc gcgtcgcggc aagctaaagg
      841 gaggaattcc gtggtcgagc ccttcggttt catcggcata ttcatcgccg tcgccttcat
      901 cctcgcgtct gtcccggttc tcctcaactg gctggtcgcg cccaaaaagc cgaatccgat
      961 caaagacgag acgtacgagt gcggcatcga aaccgtcggg gatacctggg tgcagttcaa
     1021 ggctcagtac tacatctacg cgctgatctt cgtcgtcttc gacatcgagg cggcgttcct
     1081 gctgccttgg gcagtcgcct ataagcaggt gccgctcgtc ggcgtcatcg ccatgttgct
     1141 cttcatcgtt ctcctggcgg acgggctagt gtatgcctgg cgcaaaggag acctgaagtg
     1201 gaggtaagct cccgggcgta agcccaatgc cccgcacccg gagtcgggcg acggggcgtt
     1261 tgttatcgct tgacggagaa acgatatgga tcccttcagc agcatcgccg tcgctctgga
     1321 gaacttgctc gccagcctgg ggtttgccga ctggctgatc gacttaatca tggccgtcct
     1381 gcgctcggtg atattgggga cggtcgcctt gctggcgttc atgtttctga cctggctcga
     1441 gcgcaagctg atcgggcgca tccaggatcg cctcggcccg acctacgccg gcccgatcgg
     1501 catcctgcag ccgattgccg acggtatcaa gacgctgacg aaagagatcg tggtccccaa
     1561 gggagcagac cgctggatct acttctcagg cccgatcctg gcggcgatgg cggcgctggc
     1621 cgtcttcgcc gtgattccgt tcggccccga taccggtaac ggccccctcg tcggcaccgc
     1681 gctcaacgtg ggcctggtct acgtcctggc cgtcggcgcg gccggcatcg tcgccacgat
     1741 tatggccggc tggggaagca acaacaaatt cgccgtgctc ggcacgttcc gcgccgtcgc
     1801 gcagttgatc tcctacgaag tgccccaact gttgagcctg gtggccgtgg ccatggtggc
     1861 cggcacgatg gacatggtga agatcgtcga ggggcagacg gtgccgttca tcctcccctt
     1921 attcgtcacc gcgctattgt ttctgatcag cggcgtcgcc gaatccggcc gctcgccgtt
     1981 cgatctactc gaggccgact cggagatcgt ggccggcttc cacatcgaat actcggcgat
     2041 gatgttcgcg ctggtgcaga tggccgagta catccacgcc ttcgcgatca gttgcatcgt
     2101 cgtcaccgtg ttcctcaacg gctgggcgtg gccgttcctg cccggcctgg cgccgatctg
     2161 gttcctggtc aaatgcttcg ccgtgttctt cattctgatc tggttccgtg gcacactgcc
     2221 gcgcttccgc atcgaccaga tgatggcctt caactggcag ttcatggtgc cgctggcgct
     2281 ggtcaacgtg atggtcgtcg ccgtcgtgga tcggacgctg agcgacgcag ccggctggac
     2341 gagcgcgagc cagccgtggg gctgggggct ggtgatgttc gcggccaacg tcgtgatgct
     2401 gctggcggcg ctgggcttga tgagtctcaa aggccgcgag gcgcgcatgc gtgacgcgga
     2461 gaaggcgcgc agccggagcg cagtggcggc gaccggacgc tgaactgata cgtgatgcgt
     2521 gagaggcagg cgcttcacgt gccacgcagc acttgctgga gtgggtatga ctgctcaaca
     2581 aggtttcttc gtttttttct cactcgtcac actgatcgcc ggcctgcgcg tcgtgacggc
     2641 gcgtaacctg ttccacgccg cgatctggct ggttctggcg ctggccggcg tggccgcgct
     2701 gttcgccgtg ctggacgccg ggctgctggc cgccgttcag gtgctcatct acatcggcgc
     2761 gatcgcgatc ctgattatct ttgccatcat gctcacgcgc ggtatgatgg cccccgagcg
     2821 gcgccgcctc aattcctgga gcgcgacggg cgcggtcgtg tcgctcgtgc tgctgattgt
     2881 cctggcacag gcgatcatcg ccttcccgtg ggtcacgccg ggcgcgggtg tgccggagcc
     2941 gcctgccgac agcatcgaga ccctcggcga ggcgctggtc gatccgaacc agttcgtgct
     3001 gccgttcgag ctcgcctcgg tgctgctgct cgtcgcgctc gtcggcggga ttttcgtcgc
     3061 acgcgagcgg cgcggcaacg aataggagaa acctggtttc gcgcaggctc ccgaagcgca
     3121 gcgagggggt aagcaaccag gtttctgact gagtggagga cgtgaatggt tcccttgcag
     3181 gggtatctca tcgtcggcgc ggccctgttt tgcattgggg tgttcggcgt gctggcgcgc
     3241 cgcaacgcga tcgccatcct gatgggcatc gagctgatgc tcaacgcggt caacatcaac
     3301 ctggtcgcgt tctggcgcta cgtcgagcca accgccgcga ccggtcaggt cttcgcgctg
     3361 ttcgtcctga ccgtcgccgc cgccgaggcc gccgtcggcc tcgcgctcat catcgccatc
     3421 taccggcatc gcgcctcggt gaacgtggaa gaaatcaacc tgttgaagtg gtagtcgggc
     3481 agtcagatgg tcgaatagtc acgtagtcgg atggtcaggt cctcgaccga ttatgtgatt
     3541 aggtgatcac gtgaccacgt gactacacga ctatgcgatt acgcgactaa cggactaagg
     3601 atgactatgt cgccagagac tctcgcactc atcaccctgc tcatccccat cccgccgctg
     3661 ctggcgttct tcgccatcat gttattcacg cgcaagtacc atgcggcgag ccatacaatc
     3721 gccattggcg ccgcgctcgt ctcctttgcc ctggcgctga tcatcctggt gaacgtcgtg
     3781 ccgggtggca gtgcagagct ggcagacgag ccgatccatc tgtcgttccc ctggctgcca
     3841 accggcgcca cgacgctcga cgtcggcgtg atggtcgatc cgctgacggc ggtgatgctc
     3901 gccatggtgc cgctcgtgtg tctgttgatc atcttctact cggtcggcta ctcgaacttc
     3961 aacaagaagc ccgacgagca cgatcatccc ggccagccgc cgcaccacgg gcgcgagccg
     4021 ctgtacagcc gcttcttcgc tttcctgtcg ctgttcacgg cggcgatgct cacgctggtc
     4081 gtggccgacg acctgctgct gctgttcatc ggctgggaag tgatggggct gtgctcgtac
     4141 ctgttgatcg ggttctggta cgcgcgcaat tacgacgacc cgaaccagat cacgccgcgc
     4201 caggccgccg tcaaagcctt catgaccacg cgcattgccg acgtcatcat gctgatcggc
     4261 atcgtcgcgc tgtacgcggc gacgggcacg ctgagcttcc gcgagatctt caccgaggag
     4321 acgacgcacg cgctggcgac cacgctcttc ctgggcatcc cgctcgcgca gctcaccttc
     4381 ctcctgttgt tcatcggcac aatcggcaag agcgcgcagt tcccgctgca tgtgtggctg
     4441 ccggacgcga tggaaggccc cacgcctgtc tcggcgatga ttcacgccgc cgccatggtc
     4501 tccgccggcg tgtacatgat catccgcatg ttcccactgc tagccggaac agccgagcac
     4561 ggccacgtgc cggcgttcga cgcgatgacg tggatcggcg cgttcacggc gctgatggcc
     4621 gcgacgatgg cgctaggcca gaacgacgtg aaaagggtgc tggcctattc gaccatttcg
     4681 cagcttggct acatggtcgc cgcgctgggc atcggcgctt acgtcgccgc cgcgttccac
     4741 ctgatcatgc atgccttctt caaggcgctg ctcttcctcg gctccggctc gattattcac
     4801 ggcgtagagc acggcatgca cgtggcccac gagacggccg acgtcaccca ccacgcggac
     4861 caattcctcg gctccggcgg cgcgcacggc gggccggaga agatcgatcc gcagaacatg
     4921 atgttcatgg gcggcctgcg caaaaagatg ccggtcacct tctggacgtt ccttatcggc
     4981 ggcctgtcgc tggcgggctt ccccttcgtc acctccggct tttggtcgaa ggatgaaatc
     5041 ctgggcgacg ccctgctgca cgccccgccg gtcttctggg tattggcgac tgccgcgttc
     5101 ctcaccgcgt tctacacgat gcgccagatc agcctgactt ttctcggcaa gccgcgcacg
     5161 gcggccgccg agcacgcgca cgaaagccac tggacgatga tcctgccact cgtcatcctg
     5221 gcagtcttgt ccctcgcggc cggcttcgtc ggcgtgaagg gcgatatgcc gttccttggg
     5281 ccgatcttcc gcagcggcga accgggcacg gcagtcgagg aatggcttgc gccgacgatc
     5341 gaaaatgtga tcgcgcttga agcgccggag ttcagttgga cgccggtcgc cgcgtcgatc
     5401 gtcatcgcgc tgggcggcct ggccgcgggc tactgggtct acgctcgcag gcccctggcg
     5461 gccggccagc ccgacccact ggagaggctc ggcggcgtgt acactttctt gaagcataaa
     5521 tggtacttcg acgagctgta cggccgcgtg ttcgtccgcc cgtcgatcgc gctcgcggca
     5581 gccgtctaca agtggatcga ccgctggctc atcgacggcg tcctgcacgc catcgcgcgc
     5641 ggcatgtggc gcctggccgg gtctttccgc gccttcgacc gtctcgtgat caacggcggc
     5701 gtcgactggt ccgtcgacca gctcaaggtc ttcgggcgtt ggttccgcca gacgcagacc
     5761 ggctgggtgc agcagtacct gtacgtcgtc gtaatcggcg tgctggtgct ggcggcgttg
     5821 tatctggcat tgtcattgta agtcgtaatt ggagattgga ggttagagat tggctcaatc
     5881 tccaactacc aatctccaat tggagagtga ctgatggacc tcattaacgc gaatatcctt
     5941 cccctagtca ccttcacacc gctggtgtgc gcggtgatcg tcatgctcat cccacgcgcg
     6001 gtgggtgaca ggaccgtcaa gtggatcgtc ttcgggctga gcctcgtgcc ggcggccctg
     6061 gcgatctact tgtggctcgt ctacccttcg gccccggccg acgccagcgg attcaagttc
     6121 gtcgtcgact taccgtggtt cccgcagatc aacagccggt tcatcttggg tgtggatggc
     6181 atcagcgtgt cgatgatcct gctgaccggc atcctcaccc cgctggcgat cctgatctcg
     6241 ttcagcatcc acgagcgggt caagcagtac atggcgctgt tcctgatgct ggagatgggc
     6301 ctgctaggcg tgttcgtcgc cctggacatg atcatcttct tcatcttttg ggagatcggc
     6361 ctggtgccga tgtacttcct gatcaaccag tcgggcagcg gcaaccgcga ctacgcctcg
     6421 ttcaagttct tcatctacac gatggccgga tcgatcggca tgctgctgtc tttccaggtg
     6481 atctggatgg cgctcggcgc gcagggcggc ggcacctaca gcatcgtcga gatcgcccag
     6541 cgctggccga cgttcgacgg cacgctgttc ggcctgcccg cgtcgaccgt caagtcgatc
     6601 acgttctggc tgttcacgat cgctttcgcc atcaaggtgc ctgtgtggcc gttccacacc
     6661 tggctgcccg acgcccatac cgaagcgcct accgccggct cgatgctgct cgccgccgtc
     6721 ctgctgaagc tcggcgcgtt cgggttcctg cgcatcgtcc tgccgttctt cccggccgaa
     6781 tcggcgcagt ttgcgcccgc gttggccatc ctggcgctgc tcggcatcgt gctggccgcc
     6841 ttttcggcat tcgggcagga tgacttcaaa cggctagtcg cgctgtcgtc tgtgaaccac
     6901 atgggcttcg tcgtgctggg catcgccgtc gccgcgtacg cgctgggcag cagcgatccg
     6961 gcggtgaaag agaatgccgt catcgcgacc aacggcgcgg tgctgcagat gtgggcgcac
     7021 ggcctgtcgt cggcggcgat gttcgcgctg gtcggcgtcg tgtacgatcg cgcgcacacg
     7081 cgcgacctca agcagctcgg cggattgtgg gccgtcatgc cgatctacgg cggcgtgctc
     7141 gttttcagcg cgatggctaa cctgggcttg cccgggttgg cgggtttccc tgccgagttc
     7201 tccgtcgtgc gcggcgcgtg gccggtcttc tcgctgatca cggcgctgtc gacgattggc
     7261 ctgctgttca ccggcgcctt cattctgaaa gccatcggca aagtgttgca cgggccggag
     7321 agcgaagcgg cgcatcacgt ctcggagatg aacttgaccg agcggatcgc gctggctccg
     7381 ctgctcgtcc tgatgctggc gaccggtctc ttcccggcgt gggtgctgaa cgtcgtcaat
     7441 gagactgtcg cgcggttgat cggttgatca gtaatcagtg atcagtgatc agtaatcagt
     7501 tatcgaaaat tggtgattgc tggtcacgaa caccgaggat tcgactatga cctttccctt
     7561 cctgaccgtc atcactttca cgccgatcgt cgcggccgtc atcttgctcc tgttgccgcc
     7621 cgacaagaag acactgatca agaccatcgc gctcatcgcg acggcgatca gcctggccct
     7681 gtcgatcttc gtcttcgcca cgtacgatca gcaagccggc gcaaccgatc cggcgcggca
     7741 gttccagttc caggaacagg tgcagtgggt gccgtcgctg ggcatctcgt accatgtcgg
     7801 cgcggacggc atcaacctgc cgatgatcct gctgaccggc atcgtcgcct ttggcggagt
     7861 gatgatctcg tggggcatcg aggatcgcac acgcgagttc ttcaccttct tcatgctgct
     7921 ggtggcgggc gtgttcggcg tgttcgtctc gctcgacctg tttctgctgc tgtttttcta
     7981 cgagctgtcg atcttcccga tgtacatctt gatcgccacg tggggcagca cgcgcaagga
     8041 atacgccgcg atgaagctga cgctgtatct gctgatcgga tcgatcgtcg cgctcgtcgg
     8101 cgtgctggcg atttacttct cggccaccac gcctggcggg cagcgctcct tcgacctgct
     8161 ggtgctgcgg gagtcgagca ttccgatcga ggtggcgcgc ttctggttcc tgccgatctt
     8221 cctcggcttc ggcgtgctgg ccggcatttg gccgttccac tcgtggtcgc cggacggcca
     8281 cgtcgccgca ccaacggccg tatcgatgat ccacgccggc gtgctcatga agctgggcgc
     8341 gtactccgcg ctgcgcgtcg gcgtggaggt gctgccccag gcggcgcgcg agatgctgcc
     8401 catcatcatc ttgctggtga cgatcaacgt cgtctacggc gcgtcgattg ccatggtgca
     8461 gcgtgacttc aagtacgtca tcggcttctc gtcggtcagt cacatgggcc tggtgacgat
     8521 gggcttcgcc acgctcaacc agaccgggct gaacggcgcg gtcatgcaga tgttcagcca
     8581 cggcgtgatg acggcgctgt tcttctccgt cgtcggcctg gtgtacgacc gcgcccatac
     8641 gcgcgacatt ccgagcctgg gcggtatggt ccgagtgctg cccatcgcgg ccgtcgcctt
     8701 cattatcggc ggtctggtgt cgatgggcat gcccggcctg agcggcttcg tcgccgagct
     8761 gcaggtgttc atgggcgttt gggaagggaa cgtggtcgcg gtctggtatc cgtgggtggc
     8821 gatcatcggc gcgctgggca tcatcctgac tgccgcctac gtgctgcgcg tcgtgggcct
     8881 ggtgttcttc ggcaagtacg acgagcacaa atggcacgac atgcggccgg ccaacgtgct
     8941 cgacaagacg gcgctggtga tgttttgcgc cacgatgatc gtggtgggcg tgctccccac
     9001 gctcatgatg aacatgatcc aggccggcac gacgccgttc atcgcccggc tgttgggagg
     9061 gtaaagcgtg gacctcagcc aactgaacac cggcctcctg gccgttcttc ccgagattct
     9121 gctgctggcg ctgggcttcg tcgtgatcgg cgccgacctg ttcgtcggcg cgcaccgcaa
     9181 gcgcgcgctg ggcggcttca cgacgctcgg catgctcgtc gtgctgatcg tcatcggcgt
     9241 tcagagcgca ccgctcgtcg cgggcgcgca ggcgcagccc acgctcggca acatggtcgt
     9301 cggcgatctg actacgcagt tgtttcgcgg catgttcgtg atcgcaggcg cgctcacctg
     9361 cctgatctcg atggatttca aatcgctcaa gcaggccggc gagtactacg ggttggtgac
     9421 tttcgcggtg ctgggcatgt gcctgatggc ggcggccagc gacatcatca tgctgtacgt
     9481 ggcgttggag acgacgtcca tctccctgta tctgctggcg ggctacctgc gcggcgtgcc
     9541 gctctcgtcg gaagccggca tgaagtattt cttgttcggc gcgttcacct ccacgatcct
     9601 gctgtacggc ctgagcctgc tgtacggatt caccggccag acgggttacg gggcgctcgt
     9661 cgacgccctg tcctcgctcg ggcctgaaag cgtcctgccc accgtcgtcg tggtgatcct
     9721 gattctggtc ggattcggat tcaaagtggc ggccgtgccg ttccacatgt ggacgcccga
     9781 cgtgtacgaa ggggcgccga cgccgatcac cgcgttcatc tcggtcgcct cgaaggcggc
     9841 ggggttcgcc gtgctgctgc gcgtgttcct gaccgtgttc acgccgctgc aggaccagtg
     9901 ggtcacgctc atctctatcc tggccgtgct gacgatgacg ctcggcaacc tgctcgcgat
     9961 tccgcagaga aacatcaagc gcctgctggc ctactcgtct atcgcgcagg ccggctacgt
    10021 gctgatcggc gtcgcctccg ccaggagcgg ggcgaacgcc gtgttcggcc cggcttcggc
    10081 gttgttttac ctgggcgtgt acgtgctgac gaacatcgcc gcgttcgtcg tcgtcgaagt
    10141 cgtcaccaac gagaccggca ccgagaatct gtacgagatg gccgggttga gcaagcgctc
    10201 gttctggctg gcgctgggac tgatgatggc tatgctgtcg ctgggcggcg tgcctccgct
    10261 cgcgggcttc ttcgccaagt tctacgtttt ccaggcggcc gtcggctcgg ggctggtgtg
    10321 gttggccatc gtcggcgtgc tgaacgccat cgtgggcctc tactattatt tgattgtgat
    10381 ccgggtcatt ttcgtcgatc ccggccaggg tgagctgacg gcgttcagga taccgctcac
    10441 gctgcgcacg gggctgttcg tgacgacagc gggtattttg atcctgggcg tgttggtcac
    10501 gccgtggtac aacctggtga ctcaggccgc gacgacgctg gtcagcggct cgtagtattc
    10561 gttcaatttg gcacattgac gaatgggatt gagtgtgtta acatttttat cgaagcgcgc
    10621 cccctcaata tgagggcgcg tcgtgttgta caatgaatga acgtgagccg gaaccggccc
    10681 cactcgccac cattagtaag ggactcgtcc agggcagcgc gatcggtgtg caggcgggct
    10741 gtctgggcgc agggctggtc atcggcgcgc tagtcctggg gctgtggctg gatggtcagt
    10801 tgggcacacg cccctggctc acgcttggtc tgctgtttct gagcatgccg ataagcgtgt
    10861 acgccatcta tcgagtggcg ctgcgcgcgg cgcgctcgat gcagagacgt gagcgtacat
    10921 cgaaagagga tgggcctcta ccatgacgag attccgcaag ctgatcacgc gagtcgtcat
    10981 cattgccgtg gtgatgttcc tgtgcggctg cacggctctg ttcaatctcc cggggctgcc
    11041 ccagttgttc ccggtcgtgc tgccgacgat cagcgtcgaa ccggagccgg tggcgtgcat
    11101 cggcggtggg ctgccccact gtgaaggcgg tttcccgatc acgaactcgc ttgtcgccac
    11161 gctgatcgtc gacgtgctgc tcatcgcggt gttcttcagc gcggctcgcc ggccgaagct
    11221 ggtgccgtcc ggcgtgcaga atttcctcga atcgctgatc gagattctgt acaaccaggc
    11281 cgagcaggtg gccggcagga acgctcgcat gattttcccc gtcggcgcga cgatcttcct
    11341 cttcgtcttt ttcgccaact acatggaact gctgcccggc gtcgacagcg tcggctacgt
    11401 gcatcgcgtc gaggaagagg gcgtgca
//
LOCUS       DBNHXB010000458         2931 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1321250, whole genome shotgun sequence.
ACCESSION   DBNHXB010000458 DBNHXB010000000
VERSION     DBNHXB010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1321250"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2080
                     /locus_tag="AC162G_17955"
     CDS             <1..2080
                     /locus_tag="AC162G_17955"
                     /inference="COORDINATES: protein motif:HMM:NF017539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5077745.1"
                     /translation="ALLKYHLNDKATAVINVRGPRGVGKTELAAWLAARAQEQDHRVR
                     WINCRQKDVSLQSLLVSIAVEIPNAQQADYICNPNETTQDRIAAAVAYLDTKPTLLVL
                     DDYDAIAQPKPIEALLTDIVRHARRIRVLLTAQARPECLNNPLWRPQAVAEIPVEGLP
                     RSSAGDFLGQKTFTQLSPEQLDAIWQKASGIPYLVRVLTTIVLAAGDMPDAPLLLRDE
                     HLSEWFGSFLDTLSGDARRLAYSLSVILGPLDLRIIRFLARPNEKRAAALRRELVDRH
                     VLRQIPGSGTTITYDLVRDYLYARAPTDLKRSAHASAGTYFAGVADRAQQESERLDYL
                     QQALHHFRLAPKPKDFLAKSDEVYRLLSARGDWTAAQSITEAALEFARQERKSEQIVS
                     WLLQIGTLEIDREQFEVARKHLEEALRTLPKSGHRASTQAHQQHDPLRMQLLLQRGRL
                     AYRTQDYTSAEGYFNEAIDLARDLGDQAAVADCLIRLGQIARRRSQYEAAREHLSTAH
                     TIAENASQRELAFEAVTYLGVVARRQNDVAEASRLYQAAYQMAREIGDRLKEEICLSY
                     LGRLAAHPRVGQLTTAAKFLLGALAIARDIHNTRGIRIELTRLIDVMIALQQYDRAER
                     LLAESERLNREANDEIGLAWNLKHRGQLEQRADAERGNALIREGIRTLQSLGIADEEW
                     RPEFEAALKR"
     gene            2105..2503
                     /locus_tag="AC162G_17960"
     CDS             2105..2503
                     /locus_tag="AC162G_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077746.1"
                     /translation="MHAFCTYCSADKSDEPGEIPAIRRYQSPRLSRVYEAAQQLGVGF
                     YILSGEFGLIPPECPIPWYDHLLVTTEVPALVDKLVQQIGQYGITRLVYFTQPLTQDL
                     AIAPYQDAIMTACNRLALPFLVIELGHLVK"
     gene            complement(2641..>2931)
                     /locus_tag="AC162G_17965"
     CDS             complement(2641..>2931)
                     /locus_tag="AC162G_17965"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5077747.1"
                     /translation="LKGGVTWARLTARAEEGVEFLEITYAPGATSGASMSHHEGREFG
                     LILEGALIVELGFERYTLHAGDSIVFDSTTPHRLTNAGDRPVQAIWVVLDRK"
BASE COUNT          599 a          891 c          879 g          562 t
ORIGIN      
        1 cgccctgctg aaataccacc tgaacgacaa agcgaccgcc gtcatcaacg tgcgagggcc
       61 gcgtggggtc ggcaagacag aactggctgc gtggctggcc gcgcgagcgc aagagcaaga
      121 tcaccgggtg cgatggatca actgccggca gaaggacgtt tccctccaat cattgctggt
      181 ctccattgct gttgagattc cgaatgcgca gcaggccgac tatatctgca acccgaacga
      241 gacaactcag gaccgaatcg ccgccgcggt ggcttatctg gacaccaagc cgacgctgct
      301 cgtgcttgac gattacgacg ccatcgccca gcccaagccg atcgaggcct tgttgacaga
      361 catcgtgcgc catgccaggc gcatacgggt tcttctcaca gcgcaggcgc gccccgaatg
      421 cctgaacaat cccctctggc ggccgcaggc cgtcgccgag attccggtcg aaggactgcc
      481 ccgcagcagc gccggcgatt tcctggggca aaagactttc acgcagttgt ccccggagca
      541 actggacgcc atctggcaaa aggcgtcagg cataccttac ttggtgcgcg ttctgacaac
      601 gatcgtgctt gctgccgggg acatgccgga tgcgcctctg ctgttgcgcg atgagcacct
      661 gagcgaatgg tttggctcgt ttctggatac actgagcggc gacgcgcgac ggttggcgta
      721 tagtctgtcg gtgatcctcg gccctctcga cttgagaatc atccggttcc tggcgcggcc
      781 taatgagaag cgggctgcgg cgctccgacg agagctggtc gatcggcacg ttctgcgcca
      841 gatacccgga tccggtacta cgataacgta cgatctggtt cgcgattatc tgtacgccag
      901 ggccccgacc gacctgaaac gcagcgctca cgccagtgca ggcacctact tcgccggcgt
      961 ggctgaccgg gcgcagcagg aatccgagcg gctggactac ctgcagcagg cgcttcatca
     1021 tttccgactg gcgcccaagc cgaaggactt tctggctaaa tccgatgagg tttacaggtt
     1081 gttgtctgcg cgtggtgact ggacagcagc ccaatcgatc accgaggcgg ctctggaatt
     1141 tgcgcgccag gaacgcaagt ccgagcaaat tgtgtcttgg ctgctccaga tcggaacact
     1201 tgagatcgac cgcgagcagt tcgaggtcgc ccggaaacac ctggaagagg ccttgcgcac
     1261 attaccaaaa agtggacaca gggcatccac acaggcccac cagcaacacg atccactccg
     1321 gatgcagctg ctacttcagc gtggccgact ggcctatcgc acacaggatt acacatccgc
     1381 cgaagggtac ttcaacgaag cgatcgacct tgcacgcgac ctgggcgatc aagcggctgt
     1441 cgccgactgc ttgattcggc tggggcagat cgccaggcgc cgtagtcagt atgaagccgc
     1501 gcgcgagcat ctttcgacag cgcacaccat cgcggaaaac gccagccagc gcgagttggc
     1561 gttcgaggcg gtgacttatc tgggcgtcgt cgcccgcagg caaaacgatg tcgcggaagc
     1621 ctcccgcctc tatcaggcgg cctatcaaat ggcccgcgaa atcggcgatc gattaaagga
     1681 agaaatctgc ttgagttatc tggggcggtt ggcggcgcat cctcgagtcg gtcagctgac
     1741 gacggcagcg aagttcctac tgggtgcgct ggccatcgcg cgcgacatac acaatacacg
     1801 tggtattcgc atcgagctga ctcgcctgat cgacgtaatg atcgcgctcc aacagtatga
     1861 ccgggccgag cgcctgctgg cggaatcgga gcggctcaac cgggaagcga acgacgagat
     1921 cggtcttgcc tggaatctca aacatcgagg ccagcttgag cagcgggctg acgctgaacg
     1981 tggcaacgcg ttgattcgag agggcattcg cacgcttcag tcactgggaa tcgcggatga
     2041 ggagtggcgg ccggagttcg aggcagcact caagcgttag tgacgccacg aggtgccaga
     2101 ttcgatgcat gcattctgca cctattgttc tgcggacaag agcgacgaac cgggcgagat
     2161 tcccgctatt cgtcgttatc agagccctcg tctcagccgg gtgtatgagg ctgctcagca
     2221 actgggcgtc gggttttata tcctctccgg cgagttcggc ttgatccctc ccgaatgccc
     2281 gattccatgg tacgaccatc ttctggttac caccgaagtg ccagcgctgg tggacaagct
     2341 ggtccagcag ataggccagt acggtattac gcgcttggtg tactttactc agccgctgac
     2401 gcaggatctg gctatagctc cgtaccagga cgcgattatg acggcttgca accgactcgc
     2461 gctgccgttt ctcgtgatcg aacttggtca cctggtcaag taaacaggtt aaaataagcg
     2521 gacatccgga tgtcggtggc cgagcgatca gatcgcgctc acctgcatcg ccaccacatt
     2581 gatcgccccg tggtctttct gcatgatgcc atcgacaatc acaaacgtcg agtgaatcgc
     2641 ctatttgcga tccagcacta cccagatcgc ctgcaccggt cggtcgccgg cgttggtcag
     2701 gcggtgcggg gtcgtggagt cgaagacgat gctatcgccg gcgtgaagtg tataacgctc
     2761 gaagcccagc tccacgatga gcgcgccttc caggatcaat ccgaactcgc gcccctcgtg
     2821 gtgtgacatg ctcgcgccgg aagtcgcccc tggcgcgtag gtaatctcca ggaactcaac
     2881 cccttcttcg gcgcgggccg tcagccgcgc ccaggtgacg ccgcctttca a
//
LOCUS       DBNHXB010000459         2252 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1152246, whole genome shotgun sequence.
ACCESSION   DBNHXB010000459 DBNHXB010000000
VERSION     DBNHXB010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1152246"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /gene="rsmG"
                     /locus_tag="AC162G_17970"
     CDS             <1..823
                     /gene="rsmG"
                     /locus_tag="AC162G_17970"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR5077748.1"
                     /translation="DADVDFASTDRSRYVAHADALRRVLALASYFDCIMVHSALLEGA
                     GALGLPLSPHQLAQFERYAADLVEWNRRANLTTITDPLDIVRKHFLDSLSVLSVCDVE
                     SGARIIDVGSGAGFPGLPLRIARPDLRVTLLEATRKKCDFLRHVVHVLALDNVHVVNA
                     RAEDAARDAAHRERYDVSVARAVAEAPTLLEILLPFVKVGGYALAMKSGRVESEVERA
                     GAAIAALGGRVQRFAPVSVPGIGEPRYVVVVDKVTLTPGKYPRRAGMPHKRPIGN"
     gene            complement(878..1384)
                     /locus_tag="AC162G_17975"
     CDS             complement(878..1384)
                     /locus_tag="AC162G_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR5077749.1"
                     /translation="MKSVDRVRAALVASGIGDVIIDLPDSARTAQMAAAALSKHLNQD
                     VPVGAIVKSLVFMANERPVLVLVAGDRRADADKLKAATGASTVKMADAETVRASTSYT
                     IGGVPPVGHPGSLPVIVDRSLERFETVYAAAGHPHAVFPILFTRLVEVTRGRVADVTR
                     DENPATKS"
     gene            complement(1415..1906)
                     /locus_tag="AC162G_17980"
     CDS             complement(1415..1906)
                     /locus_tag="AC162G_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077750.1"
                     /translation="MSISISESLEHEAREAAQRKGISTTEFIEAAVQSALAEFREQQI
                     AAESKAWYALPAEERRKYDGMYVAFYQGRVVDTDSDQRALHRRIRQQYGRVPVMITQG
                     GTHPVPEYIVRYLIDVHLEQMVIPAVDVVSDPNGREIVLGRNVLEKFRLLLDGPARLT
                     EIL"
BASE COUNT          408 a          696 c          689 g          459 t
ORIGIN      
        1 ggacgccgac gtcgacttcg cgtcgaccga ccggtcccgg tacgtcgccc acgccgacgc
       61 cctgcgacgg gtgctagcac tggcctccta cttcgactgc atcatggtgc actccgccct
      121 tctcgaaggc gccggcgcac tgggcctgcc gctttcgccg catcaactgg ctcaattcga
      181 gcgttacgcc gccgacctcg tcgagtggaa ccggcgcgcc aacctgacga ccattaccga
      241 tccactcgac atcgttcgca agcatttcct ggattcgctc tcagtgcttt cggtctgcga
      301 tgtggagtcg ggcgcccgaa tcatcgacgt gggcagcggc gcgggattcc ccggcctgcc
      361 gctgcgcatc gcgcgccccg atttgcgagt gaccctgctc gaggcgactc gtaagaagtg
      421 cgactttctc cggcatgtcg tccacgtgct ggcgctggac aacgtgcatg tcgtcaacgc
      481 gcgcgccgag gacgcggcgc gggatgcggc gcaccgcgaa cgctacgatg tctccgtggc
      541 gcgagccgta gccgaagcgc cgacgctttt ggagatcctg ctgccctttg tgaaggtcgg
      601 gggttacgcg ctcgcgatga agtcgggtag ggtggaatca gaagtcgagc gggctggcgc
      661 agccatcgcg gcgctggggg gtcgagtcca acgtttcgcg ccggtaagcg tgccgggtat
      721 cggcgagccg cgttacgtcg tcgtagtgga caaagtcact ctgacgcccg gcaagtatcc
      781 gcgccgcgcc ggcatgccgc acaaacgacc gatagggaac tgaccgccgc cctggggtgc
      841 cggtcgctct tcagaacggc ggagcgcgag gcgtcgttta actcttcgtc gccgggttct
      901 cgtcgcgagt cacatcggcc acgcgaccac gggtgacttc gaccagccgg gtgaacagaa
      961 tggggaagac cgcgtgcgga tgtcctgccg ccgcgtacac cgtctcaaat cgctccagcg
     1021 accgatccac gatgaccggc aggctgccag gatgtccgac gggtgggacg ccgccgatcg
     1081 tgtagctggt cgacgcgcgc acagtctcgg cgtcggccat tttgacggtc gatgcgcctg
     1141 tggccgcttt gagcttgtcg gcgtcggcgc gccggtcgcc cgctacgagg accaggacag
     1201 gccgctcatt tgccatgaac acgagcgatt tcacgatggc gccgaccggc acatcttgat
     1261 tgagatgctt gctcaatgcg gcggcggcca tctgcgccgt gcgcgcgctg tcgggcagat
     1321 cgatgatgac atcgccgatg ccggatgcaa ccagagccgc tctgactcga tcgacggatt
     1381 tcatggcgcc acggccttgt agatcatcac cgcgctaaag tatctcggtg agtcttgccg
     1441 gaccatccag cagcaggcga aacttctcca gaacgttacg gcccaggacg atctcgcggc
     1501 cattcggatc gctcacgaca tccacggcag ggataaccat ttgttcgaga tgtacgtcga
     1561 tcagataacg aacgatatac tccggcacag gatgagtgcc gccttgtgtg atcatgacag
     1621 gcacgcggcc atattgctgt ctgatgcgac gatggagtgc acgctgatcg gaatcggtat
     1681 ccactacgcg accctgatag aatgccacgt acataccgtc gtacttccgt cgttcttcgg
     1741 caggcagcgc ataccacgct tttgactcgg ctgcgatttg ctgctcgcga aactcggcca
     1801 gcgcggattg cacagccgct tcgatgaact cagttgtgct gataccctta cgctgagctg
     1861 cttcacgcgc ttcgtgctcg agcgattcgg aaatactgat actcatcgtc ttgctttcct
     1921 taagctatgt gtcttcattg atgatgatcg ttggataggg cgaacgaagc gcgtgatacc
     1981 acacattctc gcgcttctcg tctagacaaa cctctgttgc ggctgccgct ggtgtgaaga
     2041 tatggcgagt gcggaaaggt tggacgaaca tagcctcctg cactggcatc gagagcgctg
     2101 tgtacgcttc tcgcatcatg gcttcgctct caagattctt gaatagaact ctcgatggtc
     2161 tctggcgtgt tgctgcccaa ttattcgatc acatgcacgg cctgcggacc aagcaggtga
     2221 tgcaggcgag cgatcaactc atcgcagatc ag
//
LOCUS       DBNHXB010000460         6077 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1323435, whole genome shotgun sequence.
ACCESSION   DBNHXB010000460 DBNHXB010000000
VERSION     DBNHXB010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1323435"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="AC162G_17985"
     CDS             complement(<1..1388)
                     /locus_tag="AC162G_17985"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR5077751.1"
                     /translation="MSKANRSQSLRRVAITMLLPLVAVALTIAATASAAPASIGGTVT
                     LPGGGLPVPSGTHVHLLNPNLSIHGDAPVNPATGAYSFGAVPPGVYVLRAIPPQASDY
                     TPSNFQPINILYDPLTVDLQLTNPSITGTVYAPDGATPVDAWVSVFAAGRQVESRLAV
                     SGSLKVGGLFPGTYELQARRATSDPYWRSARISVTVGAGVAVQNLTLTPANVYGFVTD
                     SIGNPVPGSTVHVVRHPHRVERDVSDGNGYYAIGGLETGTYWLAVAPPWDEPGLISPL
                     PITFTVPPAQQQIDLQFLSPRKIVGGVVKTNTEHPVYHALVVARRVDKNGEARALTNH
                     SGEYLLELSEGLWAISVHRQDDSDPSRWIYPYPPKLVFFQHDQSNEHKTVNFTVLTAD
                     STVIGEVKLPDGGTPPFTVTVALHRDDGVGVETNIDASGAFTLHVPHGRYKVGVRPHD
                     PNWAGPAIEPIFA"
     gene            complement(1724..2647)
                     /locus_tag="AC162G_17990"
     CDS             complement(1724..2647)
                     /locus_tag="AC162G_17990"
                     /inference="COORDINATES: protein motif:HMM:NF016955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HIR5077752.1"
                     /translation="MPDTTVITALAKVMIAAAWADGEIDHEEVNSIKDLLFYMPNMTA
                     ADYTQVEIYLATPIGEAERARLVAELSGVVRTDADKALVMNSLTALAQSDGEVGESEA
                     AVLAEVQRAFDEGRSASGQLGRLLRGAAGRRSQTLRDAPNRENYLDDFVRNRVYYLVR
                     ERMGREAIQLDLADAELRRLSLAGALAAQVAHVGDGIDEKEKDAIARTIGTYWSLRPE
                     HAMLVAEVAASEASRGLDNFRVARELFEVTTEEERLRLIDGLFGVARSHSKVSYEQIE
                     NIRNVAALLKLSHDQFIDAKLKVPREERDGL"
     gene            complement(2817..3692)
                     /locus_tag="AC162G_17995"
     CDS             complement(2817..3692)
                     /locus_tag="AC162G_17995"
                     /inference="COORDINATES: protein motif:HMM:NF039822.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HIR5077753.1"
                     /translation="MKHKTLLVGLLVGLLALGGIGLAHAEGAKPFGHDGRRGAIIAGE
                     VTAIDGTALTLDTPHRGSIVVQTGAQTKYRAKDNPQSSLADIEVGDTIAARGHFTGEG
                     AFAARIVILVPGELSDSVRGKVIAVDGGLITVEDRDRNAVDIATSADTRFRVKGKPEA
                     SIDDVQAGMLLGAAGQFDASGALVAKQVVAVRPRGPKGGPVEVGRAAEVNGGEFALSF
                     PDGSTLTVTTDASTLVIRRGEDGASIGTPDDVKESARVVVMGIPSNGGSGIAARVIVV
                     WGKPRTQDPQAGSLP"
     gene            complement(3880..4698)
                     /locus_tag="AC162G_18000"
     CDS             complement(3880..4698)
                     /locus_tag="AC162G_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077754.1"
                     /translation="MRAVFLISVAVGIGLGLTYAWLIDPVEFTTADPYHVDARYQEAW
                     LVMAAEAYITGDDWDRTQARLDGLKDPNLAQTAAALFERYSANGPNPQARALAHLVER
                     LDARAVTAGMLVYLTTPVTPTPRATSFPASIPPTGRPAQPTPTSIDAFPTLTPSATPT
                     PEFVVVSSDGVCESGAPQIRVAVQDAEGNGLPGVDVWITWDGGADRFVTGLKPELGPG
                     FGDFDMQPDVSYRVGAGSQSTLALVGNLSADPCTTDVGAEGRLSWNVVLQPLTP"
     gene            complement(4702..5073)
                     /locus_tag="AC162G_18005"
     CDS             complement(4702..5073)
                     /locus_tag="AC162G_18005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077755.1"
                     /translation="MIRRLLLALIGLALGIGIGLFVGWFAWPATFTGAPPSVLAPGWK
                     SEAIWVAAQAYAYDGDLEAASGRLAPVFGSVDLGPIVLARAEKAIDEGFPPVEIAHLA
                     RLAAAYGARSLRTDPFLNLAP"
     gene            complement(5120..5203)
                     /locus_tag="AC162G_18010"
     tRNA            complement(5120..5203)
                     /locus_tag="AC162G_18010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5167..5169),aa:Leu,seq:caa)
     gene            5284..5961
                     /locus_tag="AC162G_18015"
     CDS             5284..5961
                     /locus_tag="AC162G_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5077756.1"
                     /translation="MSDTVLVIEDEARIADFLRRGLTFEGYKVLVAADGPSGLAVARE
                     QPPDVVILDLMLPGMDGLEVCRRLRAAESTPILMLTAKDSVTDRVTGLDAGADDYLIK
                     PFAFEELLARLRALLRRSKPAEATETLKFADLTLDPVAREAKRGERRLILTAKEFNLL
                     ELFMAHPRQVLTRETIYERIWGYDFGGESNIIEVYIRYLRQKTEAGGEPRLLQTVRGV
                     GYVLREE"
BASE COUNT         1048 a         1941 c         2004 g         1084 t
ORIGIN      
        1 gcgaagatcg gctcgatcgc cggaccggcc cagttcgggt cgtgcgggcg gacgccgacc
       61 ttgtagcggc cgtgcggtac gtgcagcgtg aacgcgccgc tcgcgtcgat gttcgtttcg
      121 acgccgacgc cgtcatcgcg atgcagcgcg acggtcacgg tgaacggcgg cgtcccgccg
      181 tcgggaagct tcacttcgcc gatcacggtt gaatcggcgg tgaggacggt gaaattgacc
      241 gtcttgtgct cattgctctg atcgtgctgg aaaaacacga gcttcggcgg atacggatag
      301 atccagcgtg agggatccga gtcatcctga cggtgtacgc tgatcgccca caggccctcg
      361 ctcaactcga ggagatattc ccccgaatgg ttcgtcagcg cgcgcgcctc gccgttcttg
      421 tcgacgcgcc gcgcgacgac gagcgcgtgg tataccgggt gctccgtgtt ggtcttgacg
      481 acaccgccga cgatcttgcg cggcgagagg aactgcagat cgatctgctg ctgcgctggc
      541 ggcacggtga aggtgatggg cagcggcgag atcagccccg gctcgtccca cggcggcgcg
      601 accgccagcc agtacgtgcc ggtttcgagg ccgccgatcg cgtagtagcc gttcccgtcg
      661 gacacgtcgc gctcgacgcg atgcggatgg cggacgacgt gcaccgtcga gccgggcacg
      721 ggattgccga tcgagtccgt gacgaatccg tacacgtttg cgggagtgag cgtgaggttc
      781 tgcaccgcca cgccggcccc gacggtgacg ctgattctgg ccgagcgcca gtacggatcg
      841 ctcgtcgcgc gccgcgcttg aagctcgtac gtgcccggga acaggccgcc taccttgagc
      901 gacccgctca ccgccaggcg cgattcgacc tgcctgcctg ccgcgaacac gctcacccag
      961 gcgtcgaccg gtgtcgcgcc gtccggcgcg tagactgtgc cggtgatgct ggggttcgtc
     1021 agttggagat cgaccgtgag tggatcgtat aggatgttga tcggctggaa gttgctcggc
     1081 gtgtagtcgg atgcctgagg cgggatggcg cggaggacat acacgccggg gggcaccgcg
     1141 ccgaaggaat acgcgccggt cgccggattc accggcgcgt cgccgtggat gctcagattc
     1201 gggttgagca ggtgaacatg cgtgccgctc ggaaccggca gcccgcctcc cggcagcgtg
     1261 actgtgccgc caatcgacgc cggcgcggcg ctcgccgtcg ccgcgatggt gagcgcgacg
     1321 gcgacgagcg gcagcagcat cgtgatcgca acccgccgca acgattgaga ccgattcgct
     1381 ttgctcatgt cttcctcctt acgatgtgca cccggcgaat cgaggcgcgc cgggccgatc
     1441 gcctgccatg gcaaatctat cttgcgttag ggaagttgcg gacgttttgc ggccatgtaa
     1501 acacgaggta aaacgaggaa gaggacaagt agtacgttgg tactccgcgc agggcaggag
     1561 aaaacgacag agacacagac ctgaggagca cgagcatttc cgcccggcgg cgtctgcggt
     1621 ccccgacctg ccgccgccgg gtgacacacc cggcgggagc agtggagaca ctctgtgccc
     1681 tctgtgtctc gatggttatt tctgagagtg agaggagacc cgattacagg ccgtcccgct
     1741 cttcgcgcgg gaccttgagc ttggcgtcga tgaactggtc gtggctgagc ttgagcagag
     1801 cggcgacgtt gcggatgttt tcgatctgct cgtacgagac cttgctgtga gagcgcgcga
     1861 cgccgaatag gccgtcgatg agccggagac gttcttcctc ggtcgtcacc tcgaacagct
     1921 cgcgcgcaac acgaaagttg tccagccccc ggctcgcctc cgatgcggcg acctcggcca
     1981 cgagcatggc atgctccggc cggagggacc agtacgttcc gatcgtgcgg gcgatggcgt
     2041 ccttttcctt ctcgtcgatc ccgtcgccca cgtgcgcgac ctgcgccgcg agagcgccgg
     2101 ccagcgacaa ccggcgcagc tccgcgtcgg ccagatcgag ctggatcgct tcgcggccca
     2161 tgcgctcgcg caccaggtaa tagacgcggt tgcgcacgaa gtcgtccagg tagttctcgc
     2221 gattcggcgc gtcgcgcagc gtctggctgc gccgcccggc cgcgccgcgc agcagccggc
     2281 cgagctggcc ggaggcggac cttccctcgt cgaacgcacg ctgaacctcg gccagcacgg
     2341 cggcttccga ttcgccgact tcgccatcgc tctgggcgag cgcggtcaac gagttcatca
     2401 cgagcgcctt gtcggcatcg gtgcgcacga caccggacag ctcggccacg agccgggcgc
     2461 gctcggcctc tccgatcggc gtcgccagat aaatctccac ctgcgtgtag tccgcagcgg
     2521 tcatattcgg catgtagaaa agcaggtctt tgatgctatt cacctcctcg tggtcaatct
     2581 cgccgtcggc ccacgcggcg gcgatcatga cctttgcgag agcggtgatg acggtcgtat
     2641 caggcatggt ctcctcatga atgcatatcc aaatatacat tataggtgga ttcaggcatt
     2701 cgcacaaagg catcttgacc ccggcgcgcc gcgctcgccg gtgggaggag agattcctca
     2761 ctgcgttcgg aatgacacgt ataggcctcg gaatgacacg gtgggtggtg tcacggctac
     2821 ggcagactgc ccgcctgcgg atcttgagtg cgcggcttgc cccacacaac gatcactctc
     2881 gccgcaatgc cgctgccgcc gttcgacggg atgcccatca cgacgacgcg cgcgctttcc
     2941 ttcacgtcgt ccggcgtgcc aatcgaggct ccatcttcgc cgcgcctgat gacgagcgtc
     3001 gaagcgtcgg tcgtcacagt cagtgtcgag ccgtctggga agctcaatgc gaactcgccg
     3061 ccgttcactt cggcggctct gcccacctcg accgggccgc ccttgggacc gcgcggccgg
     3121 accgcgacaa cttgctttgc gaccagcgcg ccgctcgcgt cgaactggcc ggccgcgccg
     3181 aggagcatgc cggcttgaac gtcatcgatc gatgcttccg gtttgccttt cacgcggaac
     3241 ctcgtatcgg ccgacgtggc gatatcaacc gcgttcctgt ctctgtcttc gaccgtgatc
     3301 aggccgccgt cgacggcgat cactttgccg cgcacgctgt cggataactc gcctgggacg
     3361 aggatcacga ttcgcgcggc gaacgcgccc tcgccggtga agtgtcctct ggcggcgatc
     3421 gtgtcaccga cctcgatgtc ggcgagcgag gactgcggat tgtccttggc gcggtatttc
     3481 gtctgcgcgc cggtctgcac gacgatcgat ccgcgatgcg gcgtgtcgag cgtgagcgcg
     3541 gtgccgtcga tcgcggtcac ttcgcctgcg atgatcgcgc cgcgccggcc atcatgtccg
     3601 aacggctttg ctccttcggc gtgcgccagg ccgataccgc ctaaggccag cagcccaacc
     3661 aacaacccta ccagcaaagt cttgtgtttc atctcagcct ccgtgggtct tccctgctta
     3721 ccgaggctga ttatcgcggc tgaatgttgt gagggaatgt ggaggttgta aaccgctggt
     3781 aaaatcgatc tgagaaccga tccgaaaact ggagtgcagg cttgagcctg ccatctgaag
     3841 cggactaaag cccgcactcc ggcggttgtc ggacagtcac taaggggtta gcggctgaag
     3901 gaccacgttc cacgatagtc ggccctcggc gccgacatcg gtcgtgcaag gatcggcgct
     3961 cagattgccg acgagcgcga gcgtggactg cgagccggcg ccgacacggt agctcacgtc
     4021 cggctgcatg tcgaagtcgc cgaagccagg ccccagctcc ggcttcagcc cggtcacgaa
     4081 gcgatcggcg cctccgtccc acgtgatcca cacgtcgacg ccaggcagcc cgttgccttc
     4141 ggcatcctgc acggcgacgc gaatctgcgg cgcgccggat tcgcacacgc cgtcggaact
     4201 gacgacgacg aattccggcg tcggcgtcgc gctgggcgtc agggtgggaa atgcgtcgat
     4261 cgacgtcgga gttggttggg caggtctgcc agtaggcggt atcgatgcag gaaagctcgt
     4321 cgcgcgcggc gtcggggtga cgggcgtcgt caggtagacg agcatgcccg cagtcactgc
     4381 cctcgcgtcc aggcgctcga cgaggtgagc cagcgcgcgc gcctgtggat tcggaccgtt
     4441 cgcgctgtag cgctcgaaga gagcggcggc agtctgagcc aggttggggt ccttcaaccc
     4501 gtcgagccta gcttgtgtcc gatcccagtc gtcgcctgtg atgtaggcct cggccgccat
     4561 caccagccac gcttcctggt agcgcgcatc gacgtgatac ggatcggcgg tggtgaattc
     4621 gaccggatcg atcagccagg cataggtcag acccagcccg attccaacgg caacgctgat
     4681 caggaatacg gcacgcatag atcagggcgc caggttgaga aaagggtcgg tgcgcagtga
     4741 gcgcgcgccg tacgcggcgg ccagccgcgc cagatgggcg atctcaacgg gcgggaatcc
     4801 ttcgtcgatc gctttttcgg cgcgcgccag gacgatcgga cccagatcga cgctgccgaa
     4861 gacgggcgcc aggcggccgc tggccgcttc gaggtcgccg tcgtacgcgt aagcctgagc
     4921 ggccacccag atcgcttcac ttttccagcc gggcgcgagc acggacggcg gcgcgcctgt
     4981 gaacgtggcc ggccaggcga accagccgac gaacaggccg ataccgatgc cgagggccag
     5041 tccgatgagg gcgaggagga gtcgtcggat cattatacaa acgggccggt ctaacccatc
     5101 ggcccgcgac gtttgccagt gccgcgggag ggactcgaac cctcacaggc tcgcgcccag
     5161 tggtttttga gaccaccgcg tataccggtt ccgccaccgc ggcatccgct ggcagtataa
     5221 ccaagaccgg gagactcgtc aagccatatt ccgcgtttcg gcggcccggt atataattca
     5281 ggcatgtcgg ataccgtcct tgtgatcgaa gacgaagcgc gtattgccga tttcctgcgc
     5341 cgaggcctga cgttcgaagg ctataaagtg ctggtcgccg cggacggccc gagcggcctg
     5401 gctgtggccc gcgagcagcc gccagacgtc gtcattctcg atttgatgct gccgggtatg
     5461 gacggcctgg aagtgtgccg ccggctgcgc gcggccgagt ccacgccgat cctgatgctc
     5521 acagccaagg acagcgtcac cgatcgcgtc accggcctgg atgccggcgc cgacgattac
     5581 ctgatcaagc cattcgcctt cgaggagctg ctggcgcgcc tgcgcgcact gctgcgccgc
     5641 agcaagccgg ccgaggcgac ggagacgctc aagtttgctg acctcacgct cgatccggtc
     5701 gcgcgcgagg cgaagcgcgg cgagcgccgc ctcatcctga ccgccaaaga gttcaatttg
     5761 ctggagctgt tcatggcgca tccacgccag gtgctgacac gtgagacgat ctacgagcgc
     5821 atctggggct acgactttgg cggggaatcg aacatcatcg aagtgtacat acgctacctg
     5881 cggcagaaga ccgaggcggg cggtgagccg cgcctgctcc agacggtgcg cggcgtgggc
     5941 tacgtgctga gagaagaatg acggatgtag cgtgtcgaat gaagaatgca gaatgaagaa
     6001 aaaagcggcg tctatcatcc cgacttaatt ctgccctcat ccgtctctca tttgcgcggc
     6061 ctagacttat caatagg
//
LOCUS       DBNHXB010000461         3370 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1659113, whole genome shotgun sequence.
ACCESSION   DBNHXB010000461 DBNHXB010000000
VERSION     DBNHXB010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1659113"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1099
                     /locus_tag="AC162G_18020"
     CDS             218..1099
                     /locus_tag="AC162G_18020"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5077757.1"
                     /translation="MSDLGFVGLGVMGSRMVKRLLDAGHAVTGYNRTKSKAQWLLDQG
                     MRWADTPRAVAEAADVTFSMVTDTTALKAIAEGSGGILAGLGPGKVYVDMSTVSPAAS
                     RDLAARVAAQGAHMLDAPVSGSVITLEEGKLSIMVGGDTGTFDKVRPVLESIGPKVTH
                     VGGNGLAVAMKIATNLSLAVQMLAFSEGVLLAEKSGIRRETAVEVLLNSVVASPMVKY
                     RGPFVLSMPDQAWFDVNMMQKDMNLALEMGRQLDVPLPTTAITNELLTAARGMGLGEQ
                     DFAVVFEVLARMAGIAK"
     gene            1147..2124
                     /locus_tag="AC162G_18025"
     CDS             1147..2124
                     /locus_tag="AC162G_18025"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR5077758.1"
                     /translation="MPGPAEPDAEQWLHMYEQMAKIREFEEKVNELYTQAIMPGLAHL
                     YIGQEAVAVGVCEALRRDDTITSTHRGHGHCLAKGASMERMFAELLGKEAGYCRGKGG
                     SMHIADQDTGNLGANAIVGGSAGIATGAAFSAKRLGTGQVAVCFFGEGALGQGLLYEV
                     MNLAQLWKLPVIYVCEDNLYNEYTHYTETTAGDLLARAKAFGITAEEVDGQDVQAVYT
                     TAQRLAERARRGEGPAFLLCHTYRYRGHHVGDVNRTYYRSKEEEQEWMALRDPLKKLA
                     DFLIGQKLTDQNVLDRIQADVKAQVEAAAQFAIGAPYPDVSQVDQHVYA"
     gene            2117..3112
                     /locus_tag="AC162G_18030"
     CDS             2117..3112
                     /locus_tag="AC162G_18030"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR5077759.1"
                     /translation="MPDANARELTLGEAIREALAEELRRDPRVFIVGEDVAEAGTPFK
                     VLSGLVEEFGPERVIDSPISEAGITGLGVGAAMTGLRPVVDIMFGDFITLAMDQMVNQ
                     AAKVHYMSGGKLKVPMVLRTTLGATRRSAAQHSQSLHAWFSHIPGLKVVVPSTPYDAK
                     GLLKTAIRDDNPVAFFEDKMMYKLKGSVPVEEYTIPFGVADVKRVGTDVTIVATSSMV
                     HVALGAAKLLEEIGISAEVVDPRTTFPLDKPALIESAKKTSRAIVVDEGYERYGVTAE
                     IASVIADGAFYYLDAPVKRLGAMDVPIPFSPPLEDATVPNEQSVFEMAKTLCGRA"
     gene            3212..>3370
                     /locus_tag="AC162G_18035"
     CDS             3212..>3370
                     /locus_tag="AC162G_18035"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HIR5077760.1"
                     /translation="MPALELAQETGKLIRWLKREGEAVAKGDPIMEIETDKVTVEIEA
                     MASGTLAGI"
BASE COUNT          660 a          992 c         1150 g          568 t
ORIGIN      
        1 gggcgcgatc ccgacgcggc ccgcgcagcg atgcgtgcgc atctgcgcca ggttcgtgaa
       61 gacagcgagg cctcggtccg cctgagcggc tgaggcttcg cgtttgggta actactcagc
      121 cgtcctgagc gcgcagggct gtctgtgcag gggattgctt cgcaaacagc gctcgcaatg
      181 acgcgggctg agcagttacg cgtttgggag gagcgagatg tccgatctcg gctttgtcgg
      241 cctgggcgtc atgggcagcc gcatggtcaa gcgactgctg gacgccgggc acgccgtcac
      301 cggttacaac cgcacgaaat cgaaagcgca gtggctgctc gaccagggaa tgcgctgggc
      361 cgatacgccg cgcgcagtgg ctgaagcggc tgacgtcacc ttcagcatgg tgacggacac
      421 gacggccctg aaagccatcg ccgaaggctc cggcggcata ctcgccggat tggggccggg
      481 taaggtctac gtggacatga gcaccgtcag cccggcggcc agtcgggacc tggccgcccg
      541 tgtcgcggct caaggcgcgc acatgctcga cgcgcccgta tcaggaagcg tcatcacact
      601 cgaagagggc aaactgtcga tcatggtcgg cggtgacacc ggcacgttcg ataaggtcag
      661 gccggttctg gaaagcatcg gtccgaaggt gacgcatgtc ggcggcaacg gactggccgt
      721 agcgatgaag atcgcgacca atctgagcct ggcggtgcaa atgctggcct tcagcgaagg
      781 tgtgctgttg gccgagaaga gtggcatccg gcgcgaaacc gccgtcgagg tgctgctcaa
      841 cagcgtagtc gcgtcgccca tggtgaaata tcgcggcccg ttcgtgctga gcatgccgga
      901 ccaggcgtgg ttcgacgtga acatgatgca gaaagacatg aacctggcgc tggagatggg
      961 gcgacaactg gatgtgccgc tgccgacgac cgccatcacg aacgaactcc tcacggcggc
     1021 gcgcggcatg ggactgggcg aacaggactt cgcggtggtc tttgaagtcc tggcgcgcat
     1081 ggcggggatt gcgaaatgag ccggacgacg ctctccattc acgatagcag cagacccggc
     1141 atccccatgc cgggtccggc cgagccggat gccgagcaat ggctccacat gtacgagcag
     1201 atggcgaaga tccgcgagtt cgaggagaaa gtgaacgagc tgtacaccca ggccatcatg
     1261 cccggactgg cgcatctgta catcggccag gaagcggtcg ccgtgggcgt gtgcgaagca
     1321 ttgcggcgcg acgacaccat caccagcacg catcgcgggc acggccactg tctggctaag
     1381 ggcgcttcga tggagcgcat gttcgccgag ctgctcggta aggaggccgg ttactgccga
     1441 ggtaaaggcg gttcgatgca catcgccgat caggacaccg gtaacctggg tgcgaatgcg
     1501 attgtcggcg ggagcgcggg tatcgccacc ggcgcggcgt tctcggccaa acggctcgga
     1561 accgggcagg tcgcggtgtg cttcttcggc gaaggcgcct taggacaggg tttgctgtac
     1621 gaggtgatga acctggccca gctctggaag cttcctgtga tctacgtttg cgaagacaac
     1681 ctgtacaacg agtacacaca ctacaccgaa acgacagccg gtgatctact ggcgcgggcg
     1741 aaagctttcg gcatcaccgc cgaggaagtg gatggccagg acgtacaggc cgtctacacg
     1801 actgctcagc gcctggccga gcgtgcgcgc cggggcgagg gtccggcttt cctgctgtgc
     1861 catacgtacc gttatcgggg tcaccacgtc ggcgacgtca accgcaccta ctaccgctcg
     1921 aaagaggaag agcaggagtg gatggcgctg cgcgacccgc tgaagaaact cgccgacttc
     1981 ttgatcggcc agaaactcac cgatcaaaac gtgttggatc gaattcaggc agacgtgaaa
     2041 gcgcaggtcg aggcggcggc gcagttcgcc atcggcgccc cgtatccgga tgtgagccag
     2101 gtggaccaac atgtctatgc ctgacgcgaa cgcccgcgag ctgacactgg gtgaagccat
     2161 tcgcgaggcg ctcgccgagg aactgcgccg cgatcctcgc gtattcatcg tcggcgagga
     2221 tgtcgcggag gccggcacgc ctttcaaagt cctgtcggga ctggtcgagg agttcgggcc
     2281 ggagcgcgtg atcgattcgc ccatttccga ggcgggcatc accggcctcg gcgtcggcgc
     2341 agcgatgacc ggcctgcgcc cggtggtgga catcatgttc ggcgacttca tcacgctggc
     2401 gatggaccag atggtgaatc aggcggccaa ggtgcactac atgtcgggtg gaaagctcaa
     2461 ggtgccgatg gtgctgcgca cgacgttagg cgcgacccgc cgctccgccg cgcagcactc
     2521 gcagtccctg cacgcgtggt tcagtcacat tcccggactc aaagtggttg tcccctctac
     2581 accctacgac gccaagggcc tcctgaagac ggctatccgt gacgataacc ctgtggcgtt
     2641 cttcgaagac aagatgatgt acaagctgaa aggctcggtt ccggttgagg agtacaccat
     2701 cccgttcggc gtcgccgacg tcaagcgtgt cgggacagac gtgacgattg tcgccaccag
     2761 cagcatggtg catgtggctc ttggcgcggc gaagctgctt gaagagattg ggatcagcgc
     2821 cgaggtcgtc gatccacgca ccactttccc actcgacaag ccggctctga tcgaatccgc
     2881 caagaagacg tcgcgagcga tcgtcgtgga cgagggctac gagcgctacg gcgtgacggc
     2941 ggagatcgcg tcggtcattg ccgacggcgc gttctattac ctcgacgcgc cggtcaagcg
     3001 gttgggcgcg atggatgtgc cgatcccctt ctccccgccg ctggaagatg cgacggtgcc
     3061 gaacgagcag agcgtcttcg agatggcgaa gacgttgtgt ggaagggcgt gagtcgcgtg
     3121 cgcgcaatgg tgaaggcggc gacggagatg gaatcgcagg cacggcatcc ccatgccgtg
     3181 caggagaggg accctggcta ctagcgttgt catgcctgcg ttggagttag cgcaggaaac
     3241 gggcaaactg atccgctggc tcaagcgtga aggcgaggcc gtcgcgaagg gtgacccaat
     3301 catggagatc gagaccgaca aggtcacggt cgagatcgaa gcgatggcct ccggcacgct
     3361 cgccggcata
//
LOCUS       DBNHXB010000462         3122 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1827190, whole genome shotgun sequence.
ACCESSION   DBNHXB010000462 DBNHXB010000000
VERSION     DBNHXB010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1827190"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="AC162G_18040"
     CDS             <1..797
                     /locus_tag="AC162G_18040"
                     /inference="COORDINATES: protein motif:HMM:NF021926.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FIST signal transduction protein"
                     /protein_id="HIR5077761.1"
                     /translation="AAIRQAGAPARALLTLAFMGPEEKLLDGATQVAPGVPVVGGSAS
                     DHTPEGKWQQYGSGHAHTGAFTIAAIGGEVGYAFSHGYHPTGKKAVITKASGRKLIEI
                     DGRPAMDVYAEWTKMPKADLGGSAILMYSVLHPLLLRKNGLTLAVHPVAGYADGSIDT
                     GVALAVGRMLELGESTVEGLIAEVEQVIKAAAQGVKKPAAVILSHCGGRAIGLGDRIG
                     EVTAQVRRAAGDIPWIGYLAFGEQGCLVPGEASHANLSLSALVLGE"
     gene            981..2654
                     /gene="ilvD"
                     /locus_tag="AC162G_18045"
     CDS             981..2654
                     /gene="ilvD"
                     /locus_tag="AC162G_18045"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR5077762.1"
                     /translation="MRSDLIKRGFDRSPHRALLRSTGAIHSDADFDKPFVAVVNSYTD
                     IVPGHVHLNHLGEVVRQAVRGAGGVPFMFHTIGVDDGIAMGHLGMKFSLPSRELIADS
                     VETMIAAHWFDAMICITNCDKIVPGMLMAAVRLNIPAIFVSGGPMKAGVTPSGTVVDV
                     SSVFEAIGAYTAGTIDEAQLKEIEDAACPTCGSCAGLFTANSMNCLCEALGMALPGNG
                     TLLAVDPRRAELARQAGRQIMKLVELDLKPRDIITADAIDNAFALDMALGGSTNTVLH
                     MLALAHEASVDYPIPRLNEIAGRVPNICRVSPSSHYRMEDVDRAGGVSAVLWELFKRP
                     GILHPDARTVTGQTLVENVRGADSKDTDCIRPLEKPYSDRGGLAILFGNLAPEGAVVK
                     TAGVVPEMMRHRGPVVVFDSHDEAHAGILGGKVKAGDVVVIRYEGPKGGPGMQEMLAA
                     TSALMGMGLGASVALVTDGRFSGATRGASIGHVSPEAAAGGPLALLRDGDVIAIDIPA
                     RQLSVELSDEELNQRRAGWQPPRKELTGWLRRYAAMVTSGSRGAVLETR"
     gene            complement(2688..2918)
                     /locus_tag="AC162G_18050"
                     /pseudo
     CDS             complement(2688..2918)
                     /locus_tag="AC162G_18050"
                     /inference="COORDINATES: protein motif:HMM:NF015714.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2943..>3122)
                     /locus_tag="AC162G_18055"
     CDS             complement(2943..>3122)
                     /locus_tag="AC162G_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077763.1"
                     /translation="RITRTGKYSGTVKAQNELAIAQVDSRILANFGGKKRPQGNRGVK
                     LILEVCSDGFSRAIR"
BASE COUNT          576 a          987 c         1073 g          486 t
ORIGIN      
        1 cggcggccat caggcaggcc ggtgctccgg cgcgggcgct gctgacgctg gccttcatgg
       61 ggccggaaga gaagctgctg gatggcgcga cgcaggtcgc gccgggcgtg ccggtcgtcg
      121 gcggcagcgc ctccgaccat acgcccgaag gcaagtggca gcagtacggc agcgggcacg
      181 cccacacggg cgcgttcacc atcgccgcga tcggcggcga ggtcggctac gccttctcgc
      241 acggctacca tccgaccggc aagaaagccg tgatcacgaa agccagcggg cgcaagctga
      301 tcgagataga tggccggccg gcgatggacg tatacgccga gtggaccaag atgccgaaag
      361 ccgatttggg tggatcggcg atcctgatgt acagcgtgct gcacccgctg ctcctgcgca
      421 agaacggtct cacgctggcc gtgcatccgg tcgccggcta cgcggacgga tcgatcgaca
      481 caggtgtggc gctcgcggtg ggccgaatgc tggaactcgg cgagagcacg gtcgaagggt
      541 taatcgccga agtcgagcag gtgatcaagg cggcggcgca gggcgtcaag aagccggccg
      601 ccgtcatcct gtcgcactgc ggcgggcgcg cgatcgggct gggtgatcgc atcggcgaag
      661 tcaccgcgca ggtacgcagg gccgcgggcg acatcccgtg gatcggctac ctggctttcg
      721 gcgagcaggg ctgtctggtt ccgggtgaag cctcgcacgc aaacctgagc ctgtcggcgc
      781 tggtcttggg cgagtagcat ccacaccgtg cgacgcacat cacacgttgg tcgtgccggc
      841 gatccgcgcc ggcacgattg tattctacgc cgctcgcagc ccgcgccccc gcgggcgtca
      901 gattccgaga agtggggcac gcctacgtgc caaaacgggc cgggcatggc ggtgctcact
      961 cctcgacaac gggaggtcac atgcgctcag acttaatcaa gcgaggcttc gatcgctcac
     1021 cgcaccgggc gctgttgaga tcgaccggcg ccatccacag cgacgccgac tttgacaagc
     1081 cgttcgtcgc cgtcgtcaac tcgtataccg acatcgtccc cggccacgtt catctcaacc
     1141 atctaggcga agtcgtgcgc caggcggtgc gcggcgcggg cggcgtcccg ttcatgttcc
     1201 acaccatcgg cgtcgacgac ggcatcgcga tgggccacct gggcatgaag ttcagcctgc
     1261 ccagccgcga gttgatcgcc gacagcgtcg agacgatgat cgccgcacac tggttcgacg
     1321 cgatgatctg catcacgaac tgcgacaaga tcgtgccggg gatgctgatg gccgccgtgc
     1381 ggctgaacat cccggcgatc ttcgtcagcg gcgggccaat gaaggccggc gtcacgccgt
     1441 ccggcacagt cgtcgacgtg agcagcgtgt tcgaggcgat cggcgcgtac acggcgggca
     1501 cgatcgacga agcacagttg aaggaaattg aagacgccgc ctgtccgaca tgcggctcgt
     1561 gcgcgggact gttcacggcc aactcgatga actgcctgtg tgaggcgctg ggcatggcgc
     1621 tcccgggcaa cggcaccctg ctcgccgtcg acccacgccg cgcagaactg gcgcggcagg
     1681 ccgggcggca gatcatgaaa ctggtcgagc tggatctgaa gccgcgcgac atcatcaccg
     1741 ccgacgcgat cgacaacgcc ttcgcgctcg atatggcgct gggcggatca accaacaccg
     1801 tgctgcacat gctggcgctg gcgcacgagg ccagcgtgga ttatccgatc ccgcgcctga
     1861 atgaaatcgc cgggcgcgtg ccgaacatct gcagggtgtc gccgtcgtca cactatcgaa
     1921 tggaagacgt cgatcgagcc ggcggggtgt ccgccgtgct gtgggaactg ttcaagcgtc
     1981 cgggtattct gcatccagac gcgcgcacgg tgaccggcca gacgctggtc gagaacgtca
     2041 ggggcgcgga cagcaaagac acggattgca tccggccgct ggagaagccg tacagcgacc
     2101 gcggcgggct ggcgatcctg ttcggcaatc tcgcgccgga gggcgcggtc gtcaagacgg
     2161 cgggcgttgt gccggagatg atgcggcacc gcgggccagt ggtcgtgttc gactcgcacg
     2221 acgaagcgca cgcgggcatc cttggcggca aagtgaaagc cggcgatgtg gtcgtcattc
     2281 gctacgaggg accgaagggc gggccgggca tgcaggagat gctggcagcc acgtcggcgc
     2341 tgatgggcat ggggctgggc gcatcggtcg cgctggtgac cgatggccgc ttcagcggcg
     2401 cgacacgcgg cgcgtcgatc ggccacgtct cgcccgaggc ggcagcgggc ggcccgctgg
     2461 cgctgctccg cgacggcgat gtgatcgcga tcgacatccc ggcgaggcag ttgagcgtcg
     2521 agctctcgga cgaagaactc aatcagcgcc gcgccgggtg gcagccgccg cgcaaagaac
     2581 tgaccggctg gctcaggcgc tacgccgcta tggttacatc cggcagccgg ggcgcggtgc
     2641 tggagacaag ataggcacct caccagcgcg cccatgatcg ccgcgcgcac gacaggcgcg
     2701 ctggcgccga cgaaaacggt gtacgcgacg atcagcagca gcaggatggg gaacgcgcgc
     2761 tggcgtccga acaaccgcac cgccggcacg gacgccggcg cgcgcaataa agaccacaaa
     2821 ctggttcttg ggaaccgaga aggagacatt ttgaggttgt ggagccgcgc tccacgaacg
     2881 gtaatctcag acataagcgg ttggtctccg ttgaacatat gcgaaagctc agtattcgcg
     2941 cttcagcgga tcgcccggct aaagccgtca ctacaaacct caagaatcaa cttgacaccc
     3001 cgatttccct ggggacgttt tttgcctccg aaattggcaa gtatcctact atcgacctgg
     3061 gcgattgcga gttcattttg cgccttgacc gtgccggaat acttgccagt ccgtgtaatc
     3121 cg
//
LOCUS       DBNHXB010000463         2572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_149859, whole genome shotgun sequence.
ACCESSION   DBNHXB010000463 DBNHXB010000000
VERSION     DBNHXB010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_149859"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="AC162G_18060"
     CDS             complement(<1..207)
                     /locus_tag="AC162G_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077764.1"
                     /translation="MRGVVALVGSGEYLDVMNDIDRRLLAESGRLNPRVVCLPTAAGL
                     EGSERVDRWSRMGVEHFHRLGAHVE"
     gene            complement(296..646)
                     /locus_tag="AC162G_18065"
     CDS             complement(296..646)
                     /locus_tag="AC162G_18065"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR5077765.1"
                     /translation="MESLAGMKCVACRKGEPTVTDAEIAEYHPQVPDWQIFEREGIKQ
                     LECVFKLPNFAEALAFTNRVGALAEEEGHHPALLTEWGQVTVTWWTHKIKGLHRNDFI
                     MAAKTDRLYEARPS"
     gene            973..1260
                     /locus_tag="AC162G_18070"
     CDS             973..1260
                     /locus_tag="AC162G_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077766.1"
                     /translation="MPPPERQARDKIDQWLTDAGWRIVPYARWQAGDRTSADAIEEYP
                     TDSGPGDYLLMLDGRTVGERTRSNSRSWRGRFAGSCNNGFSAVPATCQAQA"
     gene            1217..1669
                     /locus_tag="AC162G_18075"
     CDS             1217..1669
                     /locus_tag="AC162G_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077767.1"
                     /translation="MASQPCLPPAKRRLEDRLLLKYAQTVNGVIYAEVIVGRGGLYDW
                     PKGSKARALDGVHVLSAGRARKPADLIRYHRYNKRDIQNAIHGAVVEVIEVKAALNRV
                     VIGQVIVGADLLQMAYDPKQISSVIVCEEGDPLLELVCAKRGIRVVQV"
     gene            1779..>2572
                     /locus_tag="AC162G_18080"
     CDS             1779..>2572
                     /locus_tag="AC162G_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR5077768.1"
                     /translation="MAATAAVSAEREKRRAAQSSVAAAVFLTSIKIIVGVTTGSLGIL
                     AEAAHSALDLIAALATFLAVRLSDRPADEQHRYGHGKIENFSALVETVLLLITAAWII
                     YEAVQRLFFESAQVDASVWAFVVMGLSIAVDTAVSRALSRAAKAHHSQALEADALHFS
                     TDIWSSSVVIVGLIGVKLGEWFPALAFLGRADAIAAIVVAIIVIFVSLQMGRRTVLAL
                     LDTAPEGLPDKIKTAVEAIPGVVDCHRVRVRLSGPHLFVDAHVALAG"
BASE COUNT          471 a          803 c          795 g          503 t
ORIGIN      
        1 ttccacatgg gcgcccagcc tatggaagtg ctccacaccc atccgcgacc agcgatcgac
       61 gcgttccgat ccctccaggc cggccgcggt cggcaggcat accacgcgcg gattcagcct
      121 gcctgactcg gccagcagcc ggcgatcgat gtcgttcatc acgtccaggt attcgcccga
      181 tccaaccaac gccacgacgc ctcgcatgag ttctcctgtc gtcccggctc gcgcaagttc
      241 cttgagctcc ttgagttcct gacatgggga actgagggaa ctgaaggaac tcggctcacg
      301 atgggcgagc ttcgtacaat cggtcggttt tggcggccat gatgaaatcg ttgcggtgca
      361 ggcccttgat cttgtgggtc caccacgtaa ccgtgacctg cccccactct gtgaggagcg
      421 ccgggtggtg tccctcttcc tcggccagcg cgccgacgcg gttggtgaac gccagcgcct
      481 cggcgaagtt cgggagcttg aagacacact ccaactgctt gatgccttcg cgctcgaaga
      541 tctgccagtc gggcacctgg ggatgatact cggcgatctc cgcatcggtg accgtcggct
      601 cgcccttgcg acaggcgacg cacttcattc ctgctaacga ttccattgtg acctccctcg
      661 gctgaggcgc gaacatcttg cagggacgta ttgtatccaa aaggggataa aacgtaaaca
      721 cgcaggtgac ttctggtggg gcacattgaa ttctggggcg agtcggcggg ctggccgctt
      781 gacctcaccc ccggcccctc tcctgacgac gaaacggctg tcgtcaggag agggggaagg
      841 ggggtgaggc aaaatccgcc accaacctga aatgcacgcc tgtccgtccg ccgattgacc
      901 tatgtgccaa actcgttata gaatactgtc ataataatat gcgagcctga gcgcgacgca
      961 agaatctgaa ctatgcctcc ccccgaacga caagcgcgcg ataagatcga tcaatggctg
     1021 acagacgccg gctggcgaat cgtgccgtat gcccgctggc aggccggcga tcgcaccagt
     1081 gcggatgcga tcgaggaata cccgaccgac agcgggccgg gcgattatct gttgatgctc
     1141 gacggcagaa ctgtcggcga gcggacgagg tcgaacagtc gatcctggcg cgggcgtttc
     1201 gcggggagtt gtaacaatgg cttctcagcc gtgcctgcca cctgccaagc gcaggcttga
     1261 agacagactg ttgttgaagt acgcgcagac cgtgaacggc gttatctatg cggaagtcat
     1321 cgtcggcagg ggcggcctgt atgattggcc caaaggctcg aaggccaggg cgctcgacgg
     1381 cgtgcacgtc ctctctgccg gccgcgcccg caaaccggca gacctgatca ggtatcacag
     1441 gtacaataag cgtgacattc agaacgccat ccacggtgcg gtcgtcgagg tcatcgaagt
     1501 caaggcggca ctcaaccgcg tcgtgatcgg ccaggtcatc gtgggggccg acttgttgca
     1561 gatggcctac gaccccaagc agatcagttc ggtcatcgtc tgtgaagagg gcgatccatt
     1621 gctcgagctt gtctgcgcca agcgtggcat cagagtcgtg caagtgtgaa tgtgaagtgg
     1681 gcggaactcg gcgtgcggtt gccgggtaat gtaccttgcc ccattcgcca acctcggata
     1741 gattattctg aatcgcctgg agtctgcctg aggtctttat ggctgccacc gctgccgttt
     1801 ccgccgaacg cgaaaaacgc cgggctgcac agagctctgt cgccgcagcc gtcttcctca
     1861 cttcgatcaa gatcatcgtc ggcgtcacga ccggcagtct gggtattctg gccgaagccg
     1921 cgcactccgc gctggatctc atcgccgcgc tggcgacgtt cctcgccgtg cgcctgtccg
     1981 acaggcccgc cgacgagcag catcgctacg gccacggcaa gatcgaaaac ttctcggcgc
     2041 tcgtcgaaac cgtcttgctg ctcatcaccg ccgcctggat catctacgag gcggttcagc
     2101 gcctgttctt tgaaagcgcg caggtcgatg cctcagtctg ggcctttgtg gtgatgggcc
     2161 tctccatcgc agttgacact gccgtgtcgc gcgcgctgtc ccgcgcggcg aaggcgcatc
     2221 acagccaggc gctcgaggcc gacgcgctgc acttcagcac cgacatctgg agctcgtcgg
     2281 tggtgatcgt cggattgatc ggcgtgaagc tgggtgaatg gttccccgcg ctggccttcc
     2341 tgggcagggc cgacgccatc gccgcgatcg tcgtggcgat catcgtgatc tttgtcagcc
     2401 tgcagatggg cagacgcacc gtgctggcgc tgctggatac tgcgccggaa gggctgccgg
     2461 acaaaatcaa aactgccgtc gaggccatcc ccggcgtggt cgactgccat cgcgtgcgtg
     2521 tgcgcctgtc cggcccgcat ctgttcgtcg atgcgcacgt ggccctggcc gg
//
LOCUS       DBNHXB010000464        10422 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_485796, whole genome shotgun sequence.
ACCESSION   DBNHXB010000464 DBNHXB010000000
VERSION     DBNHXB010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10422
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_485796"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="AC162G_18085"
     CDS             complement(<1..338)
                     /locus_tag="AC162G_18085"
                     /inference="COORDINATES: protein motif:HMM:NF024668.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HIR5077769.1"
                     /translation="MSDATPRRIILTPDQRLRVFVSSTLQELAEERRAAREAIEHLRL
                     APVMFELGARPHPPRELYRAYLDQSHIFVGIYWQKYGWVAPGETLSGLEDEYRLSGDR
                     PKLIYIKSPAP"
     gene            339..932
                     /locus_tag="AC162G_18090"
     CDS             339..932
                     /locus_tag="AC162G_18090"
                     /inference="COORDINATES: protein motif:HMM:NF022700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HIR5077770.1"
                     /translation="MILLIRTICGIRGERFPQRLAASDDLNYTPGWHIARFEIRPARL
                     HSSHRRPFINSPSWTTGRSPARLILIAGDILVFVVFAVQGRATHGIPLGSSPVIATLT
                     IAAPFAVPWFAVAALMGVFRASTLAHPKQMLSRTGLAWLLAGCIGLLARAALLQRAIV
                     LPFAVTALAIIGAPLITWHGLLSVVYSRWINQQAHDG"
     gene            922..1659
                     /locus_tag="AC162G_18095"
     CDS             922..1659
                     /locus_tag="AC162G_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077771.1"
                     /translation="MTGKTLARVMIAVLAGMALGGTVVLAIGPRIFVPSQPPLPPPPT
                     ITVPRGELPAGIVAFEERVGGGLAGSGFLLELPNGDVVGVTTAHSLGEGHFAPLSFVV
                     AGRGDVAASFRALYAPLGRPHTGTHMTSDYVLMTPDAPPDSSLVLRPDPRGGPQPGER
                     VSLYSGLGDGQGGQRILHGTVERVDAGGAWVRMDDVFNPGLMSGSPMISQHTGRVVGM
                     VILMNWVPGTLRIGINPTGEIVRAAQR"
     gene            2449..2907
                     /gene="arfB"
                     /locus_tag="AC162G_18100"
     CDS             2449..2907
                     /gene="arfB"
                     /locus_tag="AC162G_18100"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HIR5077772.1"
                     /translation="MSSHLYDPWTHVIIDASLAIPLSELKFRFTLSSGPGGQHVNKAA
                     TQVELLFDVARSPSLNDAQRQRILRALERFIDSSGVLHLTSQSTRSQLRNRQDVSERF
                     QALLHQALKPRKKRRATKPTAASREQRLEKKKRRGATKRSRRTPTAEDVK"
     gene            complement(3058..4308)
                     /locus_tag="AC162G_18105"
     CDS             complement(3058..4308)
                     /locus_tag="AC162G_18105"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077773.1"
                     /translation="MIVYENIRNALSALSSNKVRSALTMLGIVIGTAAVITLLSVGQG
                     VEAFINQQFSALGTNLLFVWPRQGGSQRTQFTAFAFNQTTLTERDASALDDPLRVPDA
                     ASVAPVLRLDALAHFGRKDLITTVRGVDSDYAEVRDIQVVDGRFIGEEDVLTHARVAV
                     IGQTVRDELFSSGAEPLNQTIRINDVPFRVVGVLEARGGNQFANDDNQIIVPISAARG
                     RLTDLRSTSGLPGVSVILLQAAEAGQLDPVKTDSTATLRDTHDISYRDDDDFTILSQN
                     DLLATFGQISAAITLFLGAIAGISLLVGGIGIMNIMLVTVTERTREIGLRKAIGAKRR
                     DILIQFLVEAMTLAWLGGLMGVTLGVLGALLIAGISQEFAPRISPITLLLSTGMAVFV
                     GLVAGLYPALRAARLHPIDALRYE"
     gene            complement(4305..5546)
                     /locus_tag="AC162G_18110"
     CDS             complement(4305..5546)
                     /locus_tag="AC162G_18110"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077774.1"
                     /translation="MALRALRTNKLRSALTMLGIIIGVSAVITLLSVGRGVEAYIADA
                     FGSIGTNLLFILPGSVEEIAGSGPTQVVQDNTVLGPALTERDVEALRDRQRAPDVALV
                     SPEKQSVAILSAGGNSRRTVVSGVTPEYSAVRSWPVVAGRFVSEGDVSAQARVVVLGQ
                     TVADALFPDGRDPLQQTIRVNRVPYRVIGILERKGGSLFGDVDDQVVIPLSSAQADLA
                     SFRTRAGLPTVTFIYVQIEDDSRREAATRQIVDVMRHQHNIDYRDEDDFSVYSQAEFL
                     AAFGAITDVVTLFLSAIAGISLLVGGIGIMNIMLVTVTERTREIGLRKAIGARRRDIL
                     VQFLVEAVTLSLLGGVIGIAVGVAGALAVSRLLENFTASVTAGSILPAVGFSALVGLF
                     FGLYPALRASRLSPIEALRYE"
     gene            complement(5702..6358)
                     /locus_tag="AC162G_18115"
     CDS             complement(5702..6358)
                     /locus_tag="AC162G_18115"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077775.1"
                     /translation="MGEVAVHALRGVSLAVRGGELVSIMGPSGSGKSTLMNLIGCLDQ
                     PTSGVYRLDGVDVNKLNDNALADIRNRKIGFVFQQFNLLPRMTALENVELPLIYAHSL
                     ERRSRAQAALEAVGLGDRVKHRPNELSGGEQQRVAIARALVNDPSIILADEPTGNLDS
                     KSGGEIMAIIERLNAEQGITVVFVTHDPNVAGHTRRIIRLLDGQMVGDESVPAPAAAG
                     "
     gene            complement(6625..7986)
                     /locus_tag="AC162G_18120"
     CDS             complement(6625..7986)
                     /locus_tag="AC162G_18120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5077776.1"
                     /translation="MRRWIWIVVGLVAILAVAFFAVWRSGTPSTAAPEEADTVRVERK
                     PLVASISASGSVSPKAQVALNFGAPGTVSQVPVLEGQAVREGDVLARLDATELDLAVR
                     QAEQALVMQQIAADQVKSPRKEEVDAARAALASAQANLRALLQPDALQVDIARRQADV
                     AHESRYQVQLQWDQVKDAPVGGLRRDLLQSQYAQAVLQSEIADLQYELVARGGTEEQI
                     AAARAQVAQSQSALDRLLNDDRALRLAEAQLRQAEINLELARARLANATLTAPFDGVV
                     SEVNVVPGQAATGGLQPAIVLADLSDFHIDVGVDEIDVGRLAEGQNVTISVDALPGAS
                     ITGMVERIAPTATESAGVVSYVVTVAIDPTAAPIRAGMSAIVDIITDTRDGVLVIPNR
                     FIRIDRSTGRSYVNVKRGDQIEEIEIVTGLRNDNESEVLQGLSVGDVLIIVPITLRLP
                     FGG"
     gene            8426..9043
                     /locus_tag="AC162G_18125"
     CDS             8426..9043
                     /locus_tag="AC162G_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR5077777.1"
                     /translation="MASGEVPDIVVGRLPIYLRALSHMAAAGQEITSSHELGKNLGIS
                     SAQIRKDLSHFGEFGKQGTGYRISHLQEQIKRILKVEREWDVALVGVGDLGHAVARYA
                     GFKNRGFHITLLFDNDPAKIGARVGDLEIIDAALLKETLMAKEVKVVMIAVPASAAQQ
                     VADTCAEAGVKAILNYAPISLKVPDGVKVQHLDPVAYLQRMTYYL"
     gene            complement(9051..10070)
                     /gene="mreB"
                     /locus_tag="AC162G_18130"
     CDS             complement(9051..10070)
                     /gene="mreB"
                     /locus_tag="AC162G_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR5077778.1"
                     /translation="MFRKEIGVDLGTVNVLVHVRSRGIVLQEPSVVAMSVDQEKILAV
                     GEEARAMLGRTPDLIDVVRPMRDGVIADYRVTEAMLRYFIDKATRGGFLKSIFKPRLM
                     ISVPYGVTSVESRAVHEAGVAAGGAPRDVHLIPEPIAAALGAGLPVETPTGNMIVDFG
                     GGTTEAAVVSMNGIVAANSVRIGGVRMDEAIISYVRRKYNLVIGEPTAEEIKIRIGAA
                     LALEEELSMEVQGRDMVAGLPRTIAITSSEVTEAVQEPLAAIIAVIKSVLERTPPELS
                     SDIIDRGMALVGGGALLRKIEALVTKETSVPSYVADAPMACVALGAAKALEDYSRLRR
                     TIPGL"
     gene            complement(10094..>10422)
                     /locus_tag="AC162G_18135"
     CDS             complement(10094..>10422)
                     /locus_tag="AC162G_18135"
                     /inference="COORDINATES: protein motif:HMM:NF012619.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP synthase delta/epsilon chain alpha-helix
                     domain-containing protein"
                     /protein_id="HIR5077779.1"
                     /translation="LKEGVLRVRRGGQEEAFAIGGGIMEVLPNKVIVMADAAEHSDEI
                     DVARAEQSRARAEALIKERPAGVDLDAALAALRRSQVRLRVAQHRRRPGVPLQTRPPA
                     EGPGGA"
BASE COUNT         1951 a         3420 c         3140 g         1911 t
ORIGIN      
        1 ggcgccggcg acttgatata gatcagcttg ggtcgatcgc ctgacaggcg gtactcatct
       61 tccaagcccg agagtgtctc gccgggcgcc acccagccat acttctgcca gtaaatgccg
      121 acgaagatgt ggctctggtc gagatacgcg cgatacagct cacgcggtgg atgggggcgc
      181 gcgcccagct cgaacatcac cggtgccagg cgcaaatgct caatcgcttc gcgcgccgcg
      241 cgccgctctt ccgccagttc ctgcagcgtc gagctgacaa acacgcgcag gcgctgatca
      301 ggcgtcagga tgattcttcg gggcgtggcg tcgctcatgt gattcttcta atacggacta
      361 tctgtggaat tcgtggtgag agattcccgc agcggctggc tgcctctgac gatctgaatt
      421 atactcccgg ctggcacatc gcgcgcttcg agattcgacc agccagatta cactcaagtc
      481 ataggaggcc gtttatcaat tctccctcct ggaccaccgg gcggtcacct gcacgcctga
      541 tcctgatcgc cggcgatatt ctggttttcg tcgtcttcgc ggttcaaggt cgcgccacgc
      601 acgggatacc gctcggtagt tcgccggtga tcgccactct gactatcgcc gcgccgttcg
      661 ccgtgccatg gtttgcggtc gcggcgttga tgggtgtgtt tcgagccagc acgctcgcgc
      721 accccaagca aatgctgtcg aggacagggc tggcatggct cctggcaggc tgcatcggcc
      781 ttctcgcgcg agcggcgctc ctccagcgcg ccatcgtctt gcccttcgcc gtcactgccc
      841 tggcgatcat cggtgcaccg ttaatcacct ggcacggcct cttgagcgtc gtctattccc
      901 gatggatcaa tcaacaggcg catgacgggt aagacgctcg cgcgggtcat gatcgccgtg
      961 ctggccggca tggcgctcgg cggcacagtc gtcttggcaa tcgggccgcg aatcttcgtg
     1021 ccgtcgcagc cgccactgcc cccgccgcca accatcactg tgccgcgcgg cgagctgccg
     1081 gcagggatcg tcgccttcga ggagcgcgtg ggcgggggcc tggccggcag cggctttttg
     1141 cttgagctgc cgaacgggga cgtcgtcggt gtgacgaccg cgcacagttt gggcgaaggc
     1201 catttcgcgc cgctgtcgtt cgtcgtcgcc ggtcggggcg atgtcgctgc atccttccgg
     1261 gcactctacg caccgctagg tcgtccgcac acgggcactc atatgaccag cgactacgtc
     1321 ttgatgacgc ccgatgcgcc gcccgattca tctctcgttc ttcggcccga tccacgcggc
     1381 gggccgcagc cgggcgagcg cgtatcgctg tacagcgggc tgggcgatgg gcagggtggc
     1441 cagcgcatcc tgcacggcac ggtggagcgg gtggatgccg gtggcgcgtg ggtgcgcatg
     1501 gacgatgtgt tcaatccggg actgatgagc ggctctccca tgatcagcca gcacacgggc
     1561 cgggtcgtcg gcatggtgat actcatgaac tgggtgccgg gcacattgcg aatcggcatc
     1621 aatcccaccg gcgagattgt gcgggccgct cagcgttaag ccgtcctgct atgtcgggcg
     1681 catttcaatt tccgattttc gtagggacga ccctggcggt cgcccagggc aggtgcccag
     1741 ggcaggcgcg aggcctgctc ctacatcaaa acaggcagac tacctggacc gagttcaggt
     1801 agtctgccga aactgcaatg ttcagttaga caggaatcgc gtcaaggaga tcgggtgccc
     1861 gcagcgtcct gatcgtccgg cccccaccgg actacacgcc tgcaattagg ctattccagt
     1921 cacgcttacg agcacggcga cgtcacagct tgttgagtgc ttctttggga agttacccgt
     1981 cattcgatga cgcgcgagtt cgatgtcccc tcgccaattc gtgccaggac aattctgccc
     2041 gctcgaatga tggattagct actcgcagcc gtgcccctgt cgcctgattc ccgctggaaa
     2101 cgagctgcct cccccaaagc cctcctttcc atagccgaac gaatatcgga atgatatgcc
     2161 tcgagatgag gcgctttcag agtatcacaa ttctggatca aaatcaatag gcaaaatcaa
     2221 cagcttggct tatgagtttt gtaagaaacg tccgcctgcg tgcccggcac gcagctttga
     2281 caaaccaaga ccatataggc gtgcccgcgc gagtcgctca ataggggcaa gaaacaagga
     2341 tttctcatat gtcaatcagc atattacgta ggggatgtgg ggccctgctc gaactgttct
     2401 acataatgat tgatagggaa atcacctatc agagatataa tcacagctat gtcaagccac
     2461 ttgtacgatc cctggacaca tgtcatcatc gacgccagcc tggcgattcc tctctccgag
     2521 ctgaagttcc gattcacact gagtagtggg ccgggcgggc agcatgtcaa caaggctgcg
     2581 actcaggtcg agctcctgtt cgacgtcgcc cgctcgccca gcctgaacga cgctcagcga
     2641 cagcgcattc tgcgagcgct cgagcgtttc atcgacagca gcggcgtctt gcacctcacg
     2701 tcacaatcga cgcgcagtca gctgcgcaac cggcaggacg tgagcgagcg atttcaggcg
     2761 ctccttcacc aggcattgaa gccacgcaag aagcgccgcg ccacgaagcc cactgcggct
     2821 tcgcgggagc agcgcctcga aaagaaaaag cgccgtggtg caacgaaacg ctcgcggcgt
     2881 actcctaccg ccgaggacgt taagtgaaag gcttcggaag tctttaaggc actgcgcccg
     2941 tcagatgcct tgccaagact tccgaagtct aatgctagtg aaagacttcg gaagtcttta
     3001 aggcgctgcg cccgtcagat gccttgccaa gacttccgaa gtctaatatc tgcccgctta
     3061 ctcgtacctc aacgcatcta tgggatgtag tctcgcggcg cgcagcgccg ggtacagccc
     3121 ggcgaccagc cctacgaaca ccgccatgcc ggtcgagagc aagagggtga tcggggagat
     3181 tctgggcgcg aactcctggc tgataccagc aatcaagagc gcgcccaaca cacccagcgt
     3241 cacgcccatc agaccgccca accacgccag cgtcatggct tcgaccagga actgaatgag
     3301 gatgtcgcgc cgtttcgcgc cgatcgcctt gcgcaagcca atttcccgcg tgcgctcggt
     3361 gaccgtcact aacatgatgt tcatgatgcc gatgccgccc acgagcagcg agatgcctgc
     3421 gatcgcgccg aggaacagag tgatcgcggc gctaatctgg ccgaacgtcg ccaggaggtc
     3481 gttctgcgac aggatcgtaa agtcgtcgtc gtcgcgatac gaaatgtcgt gcgtgtcacg
     3541 caaagtcgcc gtcgaatcgg tcttgacagg atccaattgg cccgcctcgg ccgcttgcag
     3601 cagaatcacg ctgacgcccg gcaatcccga ggtggagcgc agatcggtca agcgcccgcg
     3661 cgcggcgctg atggggacga tgatctggtt gtcgtcgttc gcaaactggt tgccgccccg
     3721 cgcttccagc acgccgacga cgcggaacgg aacgtcgttg atgcgaatcg tctggttcaa
     3781 cggttcggcg ccggacgaga acagctcgtc gcgcaccgtc tgtccgatca cagccacacg
     3841 cgcgtgggtg agcacgtcct cttcgccgat gaagcgcccg tcgacgacct gtatgtcgcg
     3901 cacttcagcg tagtccgagt cgacgccgcg caccgtcgtg atcaggtctt tccggccgaa
     3961 atgcgccagc gcatcgagac gcaacaccgg cgcgacgctc gcggcgtcgg gcacgcgcag
     4021 cgggtcgtcc agcgcggagg cgtcacgctc ggtgagcgtc gtctggttga aggcaaaggc
     4081 cgtgaactgc gtgcgctgcg agcctccctg gcgcggccac acgaacagca ggttcgtgcc
     4141 gagcgcgctg aactgctggt tgatgaacgc ctccacgccc tgccccaccg acagcagcgt
     4201 aatcaccgcc gccgtgccga tcacgatgcc cagcatcgtc agcgccgagc gcaccttgtt
     4261 gctggacagc gcagacagcg cgttgcggat gttttcgtag acgatcattc gtacctcaac
     4321 gcttcgatcg ggctgagacg cgaggcgcgc agagcgggat acaggccgaa gaacaacccg
     4381 accagcgccg agaaaccgac ggcaggcagg atcgaaccgg cggtgacgct cgccgtgaaa
     4441 ttctccagga gacggctgac ggccaaagcg ccagccacgc caaccgcgat cccgatcacg
     4501 ccgccgagca aagacagcgt cacggcttcg accaggaact gaacgaggat gtctcgccgc
     4561 ctcgcgccga tcgctttgcg caggccgatc tcgcgcgtgc gctcggtgac cgtcaccagc
     4621 atgatgttca tgatgccgat gccgcccacc agcagcgaga tgcctgcaat cgccgagagg
     4681 aacagcgtga ccacgtctgt gatcgcaccg aacgccgcca ggaactccgc ctggctgtac
     4741 accgagaagt cgtcttcatc ccggtagtcg atgttgtgtt ggtggcgcat gacgtcgacg
     4801 atctggcgcg tggccgcttc gcgccgagag tcgtcctcga tctgcacgta gatgaacgtg
     4861 acggtcggca gtccggcgcg ggtgcgaaac gaagccagat cggcctgcgc cgacgacagg
     4921 ggaatcacga cctggtcgtc gacgtcgccg aagagcgagc cgcctttgcg ctccagaatg
     4981 ccgatcaccc gatacgggac ccggttgacg cgaatcgttt gctgaagcgg atcccggccg
     5041 tccgggaaca acgcatcggc gaccgtctgg ccgagcacca cgacgcgcgc ctgcgcagac
     5101 acgtcgccct cggacacaaa gcgcccggcg acgaccggcc acgagcgcac cgcgctgtac
     5161 tccggcgtca cgccgctcac aaccgtgcgt ctcgagttcc cgccggccga caagatggcg
     5221 acggactgct tttcgggcga aaccagagcc acatcggggg cgcgctggcg atcgcgcagc
     5281 gcctcgacgt cgcgttccgt cagggccggc cccagcaccg tgttgtcctg gacgacctgg
     5341 gtcggcccac tgccggcgat ctcttccacg gacccgggca aaatgaatag caggttggtg
     5401 ccaatgctgc caaaagcatc ggcgatgtag gcttcgacgc cgcgcccgac cgacaacagc
     5461 gtgatcactg ccgaaacgcc gatgatgatg cccagcatcg tcagcgccga gcgcagcttg
     5521 ttcgtgcgca gggcgcggag cgccatcgtg aagtcttcac ttaacacagg gccgcgcaac
     5581 acgatgatct atcactccca ctacttcagt gatcagttgt cggtgatcag tcgtcagtat
     5641 cagttgccag ttatcggtgt tcggtgagcg cgccacgcat cacggccgtc cgacgctttc
     5701 gctacccggc tgcggccggc gccggaaccg attcgtcgcc gaccatctgg ccgtccaaca
     5761 ggcgtatgat acgccgcgtg tgcccggcga cgtttggatc gtgcgtgacg aacacgacgg
     5821 tgataccctg ctctgcgttc agccgctcga taatggccat gatttcaccg cctgatttcg
     5881 agtcaagatt tcccgtcggc tcgtccgcca ggatgatcga cggatcgtta accagcgccc
     5941 gcgcgatggc gacgcgctgc tgctcaccgc ccgacagctc gttcggccgg tgtttgaccc
     6001 ggtcgcccag tcccaccgcc tcgagcgcgg cctgtgcgcg gctgcgccgt tccaggctgt
     6061 gcgcgtaaat cagcggcagt tccacgtttt ccagcgcagt catgcgcggc agcaaattga
     6121 actgctgaaa cacgaaaccg atcttgcgat tgcggatgtc ggccagcgcg ttgtcgttca
     6181 gcttgttcac atccacgccg tcgagccgat atacgcccga cgtcggctga tccaggcagc
     6241 cgatcaggtt catcagcgtc gatttgccgg agccggacgg ccccataatc gacaccagct
     6301 cccctccgcg cacggccagc gaaacgccgc gcagcgcatg caccgcgacc tcgcccatgc
     6361 ggtacgtctt cgtcaggttc tcgatttcga tgacgggctg gtcagatgac cgaactccca
     6421 atttcaaact ccaaacttca aaagtgtcat tccgaacgga gtgaggaatc cctcgtccga
     6481 ctcaaactcc caacttcaaa ccccaaacga caaaagtgtc attccgaacg gagtgaggaa
     6541 tctctcctcc gagcggcaag cgtagcgcac tcgggtagag atgcctcgtc gttcctcctc
     6601 ggcatgacac gacgggcgcc atctctatcc gccaaacggg aggcgcaggg tgatcggcac
     6661 aatgatcaac acgtcgccca cactcaatcc ttgcagcact tcgctttcgt tgtcattccg
     6721 taggccggtg acgatctcga tttcctcaat ctggtcgcct cgcttcacgt tcacgtaaga
     6781 gcggcccgtg ctgcgatcga tgcggatgaa gcggttgggg atgaccagca cgccatcgcg
     6841 tgtgtcggtg ataatgtcga cgatggcgct catgcccgcg cgaatcggcg cggcggtcgg
     6901 atcgatggca actgtcacga cgtacgacac cacgccggcg ctctcggtcg ccgtaggcgc
     6961 gatacgctcg accatacccg taatcgacgc acccggcagc gcgtcgaccg agatagtcac
     7021 gttctgccct tcggccaggc gccccacatc gatctcgtcc acacccacgt cgatgtgaaa
     7081 atccgacagg tcggcaagca cgatcgccgg ctgcaagccg cccgtcgctg cctgaccggg
     7141 cacgacgttc acctcagaga caacgccgtc gaagggagca gtcagagtcg cgtttgccag
     7201 tctggctctg gcaagctcca ggttgatctc ggcctgccgc aactgagctt cggccagccg
     7261 cagcgcgcga tcgtcgttca ggagccgatc gagcgccgac tgcgactgcg ccacctgggc
     7321 gcgggctgcg gcgatctgct cttcggtccc gccgcgtgca accaactcgt attgcagatc
     7381 ggcgatctcc gactggagca cggcctgggc gtactgcgac tgcagcaggt cgcgtctcag
     7441 cccacccacc ggcgcatcct tgacctggtc ccactgcaac tgcacctgat atcgggactc
     7501 atgcgcgaca tccgcctggc gccgggcgat gtcgacctgg agcgcatcgg gttgaagcaa
     7561 cgcgcgcaga ttggcttgcg cgctggccag cgccgcgcgc gcggcgtcca cctcctcttt
     7621 gcgcggggac ttcacttgat cggcggcaat ctgctgcatg accagcgcct gctcggcctg
     7681 gcgcacggcg agatcgagtt ccgtcgcatc gagacgggcc agcacgtcgc cctcgcgcac
     7741 cgcctgccct tccaagacgg gaacctggct caccgtgccc ggcgcgccga aattcagggc
     7801 gacctgcgct ttcgggctta cgctgccgct ggcgctgatc gacgcgacga gcggcttcct
     7861 ctccacgcgc accgtgtcag cttcttccgg cgcggcagta cttggcgtgc ccgaacgcca
     7921 gacagcaaaa aaggccaccg ccagaattgc gacgaggccg acgacgatcc atatccaacg
     7981 acgcatcccc tactccttgc tatggcctgg agtttggcac gtgccatatc ttgggccgga
     8041 atcgaggcat cagccggtgc tgaccgccag acatgtccga caaaagcctc gactccggcg
     8101 atttgccaaa gtccagtatg gtccgaggca ggggcgtgtt cccatcccca aggatcgcac
     8161 agtagggcga gccaacgctc gccctacccc gcaccaggac ctgcattttt cacgccgcaa
     8221 actgcgccgg gcgctcattc gcgagagatt tcgcaaacat tcggattata ccgcccacca
     8281 tgggcagcgt caatcacagg cggccgggtg atcgcttaca aagtgcgtgg ctccgtttgc
     8341 ggcgcatggt gcttcgtggt atattattga ccaattcttc acaaaattgt gattgtaccg
     8401 aacttaagcc aaggagtggg cgcctatggc atccggtgaa gtgcccgaca tcgtggttgg
     8461 aaggctgccg atttatttgc gcgctttgag tcacatggca gccgccggac aggaaatcac
     8521 ctcgtctcac gaactcggca agaatctggg catatcgtcg gcgcagatcc gcaaggattt
     8581 gtcacacttc ggcgagttcg gcaagcaggg taccggctac cgcatcagtc acctgcaaga
     8641 gcagatcaag cgcattctca aagtcgagcg cgagtgggac gtcgcgctcg tgggagttgg
     8701 cgatctgggg cacgccgtcg cgcgctatgc cggattcaaa aatcgcggct ttcacatcac
     8761 gctactgttc gacaacgatc cggcaaagat cggcgcgcgc gtcggcgatc tggagatcat
     8821 cgatgccgcg ctgctcaaag aaacgctgat ggcgaaggag gtcaaagtgg tgatgatcgc
     8881 cgtgccggcg agcgccgccc agcaggtcgc agacacgtgt gccgaagccg gcgtgaaagc
     8941 catcctcaat tacgccccca tctcgctgaa ggtgccggac ggcgtgaaag tgcaacacct
     9001 cgatccggtc gcctatttgc agcgcatgac gtactacctg taactgagac ttacagtccg
     9061 gggatggtgc gccggaggcg gctgtagtct tccagggctt tggctgcgcc gagtgcgacg
     9121 cacgccatcg gcgcgtcggc gacgtagctg ggcacgctag tctccttggt gaccagggct
     9181 tcgattttcc gcagcagcgc cccgcccccg accagcgcca tgccgcgatc gataatgtcg
     9241 ctggacagct cgggcggcgt cctctccagc acgctcttga tgacggcgat gatggctgcc
     9301 aaaggctcct gcacggcctc ggtcacttcc gacgacgtga tggcaatcgt gcgcggcagg
     9361 cccgcgacca tatcgcgccc ctgcacttcc atcgacagtt cctcttccag cgccagcgcc
     9421 gctccgatgc gaatcttgat ctcctcggcc gtcggctcgc cgatgacgag attatatttc
     9481 ctacgaacgt aggagatgat ggcctcgtcc attctcactc ctccgatccg cacggagttg
     9541 gcggcgacga tgccattcat cgacacgacg gcggcctccg tcgtgccgcc gccgaagtcg
     9601 acgatcatat tgccggtggg cgtctcgacc ggcaggccgg cgcccagggc ggctgcaatc
     9661 ggctcgggga tcaggtgcac atcgcgaggc gcacccccgg ccgccacgcc ggcctcgtga
     9721 acggcgcggc tttccacgct cgtcacgccg taggggacgc tgatcatcag gcgaggcttg
     9781 aagatggact tgaggaagcc accgcgcgtg gccttgtcga tgaaataccg cagcatggcc
     9841 tcggtcacgc gatagtcggc gatgacgcca tcacgcatgg ggcgcacgac gtcgatcaga
     9901 tccggggtac ggcccagcat cgccctggcc tcttcgccga cggcgaggat cttctcctgg
     9961 tccaccgaca tggcgacgac cgacggttct tgaagcacga tgccgcggct gcggacatgc
    10021 accagcacgt tgaccgtgcc caggtcaacg ccaatctctt tccgaaacat tcagtctctc
    10081 cggccggagc gtctcacgcc ccgccaggcc cctcggcggg cgggcgcgtc tgcaatggca
    10141 cgccggggcg ccggcggtgc tgggcaaccc tgaggcgcac ttgcgagcgg cgcagcgcgg
    10201 cgagcgcggc gtccagatcg acgccggccg ggcgctcttt gatcaacgct tcggccctcg
    10261 cgcgcgattg ttcggcgcgg gccacgtcga tctcgtcgga atgctcggcc gcatcggcca
    10321 tcacaatgac cttgttggga agcacctcca tgatcccgcc gccaatcgcg aaagcctcct
    10381 cctggccgcc tcgcctgacg cgcaaaaccc cttccttcag gt
//
LOCUS       DBNHXB010000465         5431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3841061, whole genome shotgun sequence.
ACCESSION   DBNHXB010000465 DBNHXB010000000
VERSION     DBNHXB010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3841061"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="AC162G_18140"
     CDS             complement(<1..412)
                     /locus_tag="AC162G_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5077780.1"
                     /translation="MTTQMNAIEVQNLHRSRGEVKAVQGVTFSVSQGEIFSLLGPNGA
                     GKTTTILVLSCLLRPDEGDALVMGHSVRRDPMGVKSVLGVVPQDLALYEDLSARENLT
                     FWGKMYGLRGAALKRRVDEVLDIIGLSDRAKGRVG"
     gene            complement(557..1840)
                     /locus_tag="AC162G_18145"
     CDS             complement(557..1840)
                     /locus_tag="AC162G_18145"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR5077781.1"
                     /translation="MDTTLPLRENAEEALRLQNARLAALHQVALGLTSTLDLREVLQR
                     VVVMAQTLSASAHAHIFLYDSERDELNLAASHWSADQRSVPLQPRRTGVTHSVARSGT
                     PEFIENLSNHPAYAHAPPDARPGALACLPLVKADHILGTLNLGYWEPHRFDADTRGFL
                     DLMARQAAIAIETARLHADAIEKARLERELQVARGLQTSLIPRETPRVAGWDFAALWQ
                     PAHVVSGDFYDFVPVHDVPGLGIIIADVSDKGMPAALYMALARSTIRASITSACCPAD
                     CVTHANRVLCADTENGMFVTMCYAQLNPATGELVYVNAGHNPPLLYRQDRRQLTELAR
                     TDMVLGVDDTHCYEQRTLQLDPGDFVVFYTDGVTEAANVTEQEFGKEHLRQVVLSARG
                     ASAVDIIAALRRAVDEFIGSMPPFDDVTAVVVKRL"
     gene            complement(1870..2658)
                     /locus_tag="AC162G_18150"
     CDS             complement(1870..2658)
                     /locus_tag="AC162G_18150"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077782.1"
                     /translation="MAATSALQPVREKGWRSGFANLLSKENGAWWRTRRWLVQTIIWV
                     AILNGILATILFAEGVADPPSEGASLFMILSLAVLPMGVIIIAQEAIIDEKRSGTAAW
                     VLSKPVSRAAFILSKLIAHGLGLLVTGVVIPGTIAFILLSAAGGSIAPGDFVAALGIA
                     FLNVLFYLTLTLMLGTLFDGRGAVIGIPMAFLFSYQIFVGLAPWLADITPWGFLVGAG
                     SPVPPIAQVALGQGALPLAPILATALWCVLFMSVALWRFAREEF"
     gene            complement(2645..3613)
                     /locus_tag="AC162G_18155"
     CDS             complement(2645..3613)
                     /locus_tag="AC162G_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077783.1"
                     /translation="MNGSTPVVIDTRGLSKTYNGTQALQSLDLKVHKNSIFGFLGPNG
                     AGKTTSIKLLLGLARPTSGGGTVFGHDIARESGSIRQRVGYLAQDPRFYEHMTARETL
                     RFTARFFYRGPKAAIEARIQNTLELVGLEDKADRPIKGFSGGERQRLGIAQAQVNYPD
                     LLILDEPAASLDPQGRRDVLEVMERLRKYTTIFYSTHILADVQRVSDTVAILNRGRLV
                     AQAPIEQLLAGSEGTAYTVVVKGDGERAREHVASQTWVKSVSVAPGNDLTAWNVSVND
                     EQVAERQLLRLVLSDESLVVTEFGRKKYELEEIFLNIVEGSNHGRH"
     gene            complement(3810..4031)
                     /locus_tag="AC162G_18160"
     CDS             complement(3810..4031)
                     /locus_tag="AC162G_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077784.1"
                     /translation="MNPVYEIRVKGHLDPRWSEWFQGLTVTRTASGETILSGSIVDQA
                     ALHGLLAKIRDLNLTLISVTRAEAEQANE"
     gene            complement(4028..4468)
                     /locus_tag="AC162G_18165"
     CDS             complement(4028..4468)
                     /locus_tag="AC162G_18165"
                     /inference="COORDINATES: protein motif:HMM:NF016001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HIR5077785.1"
                     /translation="MPGAIGNFFMKAIISSPLHPLLGANFAVVTVEGRKTGRLYSTPV
                     NAVKDGDIFTVVSLRSRSWWRNLRGGRPARLRASGKQYSVRGEVLESHGEVVDGLAHF
                     LQRHPGYAKYYGVRLASDGRPMQDDLERVADERVLIRLSSAVPQ"
     gene            complement(4484..5335)
                     /locus_tag="AC162G_18170"
     CDS             complement(4484..5335)
                     /locus_tag="AC162G_18170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR5077786.1"
                     /translation="MIAQTRKQTTVSTWIKQHPVSAYFILAIAITWSIMLPLVASAQG
                     WLQAPIPFALHYLAAFGPMLAALIMTWATGGVAGLQELWGRVTKWRVGLTGAAFAILS
                     PVVLFVLAAIVVRALGGEWPDLQRLGEVNYLPNLGIGALVLWLATYGFGEEIGWRGFA
                     LPRLQKSRSALSATLILGVMWIVWHMPAFFYLDTYVALGLAAFPLFALGVMTGAIVLT
                     WLYNTTGGSVLMVAVWHGVFDFLSASKANDGTIAALMSAAIMVWAVVIVIVFKPANLA
                     RHEKHTL"
BASE COUNT         1015 a         1732 c         1712 g          972 t
ORIGIN      
        1 ttccgacgcg ccctttggcg cgatcgctca ggccgatgat atccagcacc tcgtccacgc
       61 gccgcttgag ggccgcgccg cgcaggccgt acatcttgcc ccagaacgtg aggttctcgc
      121 gcgccgagag gtcttcgtat agcgcgaggt cctgcggcac aacgcccagc acagacttga
      181 cgcccatcgg atcgcggcgg acggaatggc ccattaccag cgcatcgccc tcgtcagggc
      241 gaagcagaca ggaaagcacc aggatggttg tcgtctttcc ggcgccgttg gggccgagca
      301 ggctgaagat ctcgccctga ctgacgctga aagtgacgcc ctgcacggcc ttgacctcgc
      361 cgcgggacct gtgcaggttt tggacttcaa tcgcgttcat ttgagttgtc atctttctct
      421 cctggtcatg actgaggctc gacccgtgcc accgatgcct atgcctgcta aaggaatttg
      481 agcaggccgt gcaggatggt cgggccgcac gctgcccgtc ttgtctcgaa tgacaccggc
      541 gataaggccg acgccattac aggcgtttga ccacgacggc ggtcacgtcg tcgaagggcg
      601 gcatggaacc gatgaattcg tcgacggctc gtcgcagtgc ggcgatgatg tccaccgccg
      661 atgcgccgcg tgcggagaga acgacttgcc gcaggtgctc cttgccaaat tcctgctccg
      721 tgacattggc agcctcggtg acgccgtccg tgtagaacac gacgaaatcg cccggatcga
      781 gctgcagggt gcgttgctca taacagtgcg tgtcgtcgac gcccagcacc atgtctgtgc
      841 gcgcgagctc ggttagttgc cggcggtctt gacgatacag caagggcgga ttgtgtcccg
      901 cgttgacgta cacgagttcg cctgtcgccg gattgagttg cgcgtaacac atcgtgacaa
      961 acatgccgtt ctccgtgtcc gcgcacagca cgcgattggc gtgagtgaca caatcggcag
     1021 gacagcacgc gctggtgatg ctggcgcgga tcgtgctgcg cgccaacgcc atgtacagag
     1081 cggcgggcat gcccttgtct gagacgtcgg caatgatgat gcccagcccc ggcacgtcat
     1141 gcaccggcac gaagtcgtag aagtcgccac tcaccacgtg ggccggctgc cacaaggcgg
     1201 cgaaatccca gccggcgacg cgcggtgttt cgcgcgggat cagactcgtc tgcagcccac
     1261 gcgccacttg cagttcgcgt tccaaacgcg ctttttcgat cgcgtcggca tgcagccgcg
     1321 cggtctcgat ggcgatggct gcctgccgcg ccatgagatc gagaaaaccg cgcgtgtcgg
     1381 cgtcgaagcg atggggctcc caatagccga ggttgagcgt gccgaggatg tgatcggctt
     1441 tcaccagcgg cagacaggcg agtgcgccgg gtcgcgcatc gggcggcgcg tgcgcgtagg
     1501 ccggatgatt cgacaggttt tcgatgaatt ccggcgtgcc gctgcgcgcg accgaatggg
     1561 tgacgcccgt gcggcgcggc tgcagcggca cactgcgctg atcagccgac cagtggctcg
     1621 cagccaagtt cagctcatcg cgctcggaat cgtacaggaa gatgtgcgcg tgtgcggagg
     1681 ccgacaacgt ctgcgccatt acgaccacac gctgcaagac ttcgcgcaag tccagtgtcg
     1741 acgtaagccc taacgccacc tggtgcagcg cggccagccg ggcattctgc aggcgcagcg
     1801 cttcttcggc gttctcgcga agcggaagcg tcgtgtccat tccagccggc acctgctcga
     1861 cgaagcgcgt cagaactctt cgcgcgcaaa gcgccacagt gcgacggaca tgaacagcac
     1921 gcaccacagc gcggtggcga ggatcggcgc caggggcaag gctccttggc cgagcgccac
     1981 ctgtgcgatc ggtggaacgg ggcttcccgc cccgacgagg aagccccacg gcgtgatgtc
     2041 ggccagccac ggcgcaaggc cgacgaagat ctggtagctg aacaggaaag ccatcggtat
     2101 tccgatgacc gctccgcgcc cgtcgaagag cgtgccgagc atgagtgtga gcgtgaggta
     2161 aaacagcacg ttcaggaacg cgatgcccag cgcggcgacg aaatcgccgg gggcgatcga
     2221 gccacccgcc gccgagagca ggatgaaggc aatcgtgccc ggaatgacca cgccggtgac
     2281 gagcaggccc agcccgtgcg caatcagctt cgacaggatg aaggctgcgc gcgacacggg
     2341 cttggagagc acccacgccg ccgtgcccga ccgcttctcg tcgatgatgg cctcctgcgc
     2401 gatgatgatc acgcccatgg gcagcacggc caggctcaag atcatgaata acgacgcgcc
     2461 ctcggaagga ggatcggcca ccccctccgc gaacaggatc gtggcgagga tgccattcaa
     2521 gatggccacc cagatgatcg tctgaaccag ccagcggcgc gtgcgccacc acgcgccgtt
     2581 ttctttgctc aacaggttgg cgaagccgct tcgccagcct ttctcgcgga cgggttgcag
     2641 tgcgctagtg gcggccatgg ttgctccctt cgacgatgtt gaggaagatc tcttccagtt
     2701 cgtacttctt gcgcccgaac tcggtcacga cgaggctctc gtcggacaga accagtcgca
     2761 gcagttgtct ctcggcgacc tgctcatcat tcacactgac gttccacgcc gtcaggtcgt
     2821 tgcctggcgc gacgctgacc gacttgaccc acgtttggct ggccacgtgc tcgcgcgccc
     2881 gctcgccatc tcccttgacg acgacggtgt acgccgtgcc ttcgctgccg gccaggagtt
     2941 gctcgatggg cgcctgcgcc accagccgcc ctctgttcaa aatggcgacc gtgtcgctca
     3001 cacgctgcac gtcggcgagg atatgcgtcg agtagaagat ggtggtgtat ttgcgcaggc
     3061 gctccatcac ttcgagcacg tcgcgccggc cctgcggatc gagcgaggcg gccggctcgt
     3121 cgaggatcaa caggtcggga tagttgactt gcgcctgggc gattcccagc cgctggcgct
     3181 cgccgccgga gaagcccttg atcgggcggt cggctttgtc ctccagcccg acgagttcca
     3241 gggtgttctg gatgcgagct tcgatggcgg ccttcggccc gcggtagaag aagcgcgccg
     3301 tgaagcgcag cgtctcgcgg gcagtcatgt gctcgtagaa gcgcgggtct tgcgccaggt
     3361 agccgacgcg ctggcggatc gacccgctct cgcgagcgat gtcgtgaccg aagaccgtgc
     3421 cgccgccgct cgtcggacgc gccagtccga gcagcagctt gatcgacgtg gtcttgcccg
     3481 cgccgttcgg cccgaggaag ccgaagatcg agttcttgtg gaccttcaaa tcgagcgact
     3541 gcagcgcctg tgtcccgtta taggtcttgc tcaagccgcg cgtgtcgata acgacgggtg
     3601 tggatccgtt catcttgaat gtctcctttg ggattcatca atgggcaggc attcctgccg
     3661 agctggcttg tgctagaaac agtatagatg gacacaccgc gcttgccatc tgacgaaagt
     3721 cgcatcccat gtacgacttt cgacactgga tatttctcac cgtgatgagc gtaatcctct
     3781 gaaggggaca ggcggacgcg atggacaacc tattcattcg cctgttcggc ttcagcgcga
     3841 gtcactgaga tcaaagtcag attcagatcg cggatcttcg caagcaagcc gtgcagcgcc
     3901 gcctggtcga cgatcgagcc ggacaggatc gtttcgccgg acgccgtgcg ggtcaccgtc
     3961 aagccctgga accactccga ccaacgaggg tcaaggtggc ctttgacgcg gatctcataa
     4021 accggattca ctgcggcacc gccgagctga gccgaatgag gacgcgttcg tccgccactc
     4081 gctcgagatc atcctgcatc ggccggccat cggaggccag gcgcacgccg tagtactttg
     4141 catagccagg atgccgctga aggaagtgcg ccaggccgtc gacgacttcg ccgtgactct
     4201 cgagcacttc gccgcgaaca gaatactgct tgcccgacgc ccgcagtctg gctggccgcc
     4261 cacctctgag gttgcgccac cagctcctgc tcctcagact cacaacggtg aagatgtcgc
     4321 cgtctttcac ggcattgacg ggtgtggaat acagcctacc cgtcttgcgc ccctcgacgg
     4381 tgacgacggc gaagttcgcc cccagcaggg gatgcaaagg cgaactgatg atcgccttca
     4441 tgaagaagtt gccgatcgcg cctggcatgt tgacctcctg gcctcacagc gtatgcttct
     4501 catgccgggc gagattggcc ggcttgaaca cgatgacaat cacgaccgcc cagaccatga
     4561 tcgccgcact catgagcgcg gcgatggtgc cgtcgttggc cttcgatgcg gagaggaaat
     4621 cgaacacgcc atgccacacg gcgaccatga ggacgcttcc cccggtcgtg ttgtacagcc
     4681 aggtcaacac gatcgcgccc gtcatgactc ccaacgcgaa cagggggaag gcggcgaggc
     4741 ccagcgccac gtaagtatcc aggtagaaga aggccggcat gtgccacacg atccacatca
     4801 cgccgaggat cagcgtggcc gacagcgcgc tgcggctctt ctgcagcctg ggcaaggcaa
     4861 aaccgcgcca gccgatctct tcgccgaagc cgtaggtcgc cagccacaaa accagggcgc
     4921 cgattcccag attcgggagg tagttgactt ctccaaggcg ctggaggtcg ggccattcac
     4981 cgcccaacgc ccgaactacg atggctgcca gcacaaacag caccacgggc gacaagatcg
     5041 cgaaggccgc gccggtcagc cccacgcgcc atttcgtgac ccgcccccat agctcttgca
     5101 ggccggcaac cccgccggtg gcccaggtca tgataagcgc ggccagcatc ggcccgaagg
     5161 cggccaggta gtgaagggcg aacgggatag gcgcttgcag ccagccctgc gccgaggcca
     5221 ccagcgggag catgatgctc cacgtaatgg caatagcgag gatgaagtag gcggaaaccg
     5281 gatgttgctt gatccaggtg gaaacggtcg tctgcttgcg tgtctgtgcg atcatgttgt
     5341 cctcccgctt ggttttgctg tgctcgcccg actttctatt cgggtgatga gactttgccg
     5401 gtgattcatt tgcacccaca gcctaaccct a
//
LOCUS       DBNHXB010000466         3967 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2838237, whole genome shotgun sequence.
ACCESSION   DBNHXB010000466 DBNHXB010000000
VERSION     DBNHXB010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2838237"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2130)
                     /locus_tag="AC162G_18175"
     CDS             complement(<1..2130)
                     /locus_tag="AC162G_18175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077787.1"
                     /translation="MSTRIRRTVFICVIAVSALWLSFELALSRPQKPGFSEGAGFSAA
                     PLVSQQQTCQELFKPTDIPAPGLINFDDLANAAIIGDHYRAAYGVTFESNSTTQAIVY
                     GNEPAQAQSPPNVASNNAVFPNTSSGVSMRIFFDRPKTHVGFWMGNGDTLGPIGLMTA
                     YDAFNAVICQVSNAPVPEPHTEFIGIYDPEGRIASVSLDYGVTALSESIDDLYFAPYA
                     PTLTPPVRAAGYTAYALAGGDRLHYPYVARNAGNGWTSMLAARNMLSVPVPVTLTIHG
                     GALQLTATAMLAGNGSHIFDLAFMAQLPDNFIGSATLDAPPGGGSLVGAALVRSATQP
                     GDAFFAYDAIPAGTAFSRLWAPIDSDGEGSPDHRHTIIAVQNADNVASASVIVRYFDE
                     TGSYLPAATSLYLIPPNALLTIDSALNPNLVNFSGVALIESTGAGLVGLTINTNDFGD
                     TYATEAWPGLFEGSEFLLLPLFNQPFGVKSDLWIRSVVPVAQNLTLSYYSPSGTLNSE
                     FRAALPPTGTLRHLVVDDTAVWDDPFIGTISFSPWGFGHTVNQRGNAQVGVNWFDRPS
                     LAFGDVHVPLVVNDASIETELVIGASAGQGGPVEVAFFDDDGALVYRHIISNLNLLGT
                     ARIRTADPPHDAALHNFRGSAVVRSLVNDALAVQVTLLRLPSGAGFDNFEPNDRPEDA
                     YPIRARVVYTSFLSHPRDFDWYAIYLTD"
     gene            complement(2209..>3967)
                     /locus_tag="AC162G_18180"
     CDS             complement(2209..>3967)
                     /locus_tag="AC162G_18180"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5077788.1"
                     /translation="SIEMPDTIHGVIMGRIDRLPEPARRALQVAAVVGRVVDLDLLHA
                     VYDVPGENLESLRRHLSDLADLGLLPVESGSSQARYAFRSVTTQEVAYDSLRYERRRE
                     LHRRIGEVLEKTSALGDDRSGLLTHHFFEGQMWDKALGYALEAGWCAQHQYANTAAAA
                     AYRRALLAAERLSPPRRLEQLAAHEALGDLLSITGEYDEALRSYERARALAANDARHL
                     ADLCRKTAETHEKRSEYEEAFTWLNQGLEHAAADSLEAARIYLMGAGVFHRQGRHDQA
                     AEWSQKSLATSRILPDDAARRTEAHAQYLLAGIHYKRGDLEQAIESAQRAATVYLLAG
                     DMLGQSQALNTLASAYQAVGQLIQATDYFQQGLQIKEQIGDVAGQAMITLNLGEIYRA
                     KGEWAAARQMQTQSLRIWRDLRNSYAIALLHNNLAATAIAERKWEEAAERLAESEKLF
                     AEIGSEDFKAELLRHRAELSLGQGRVVEALNRAQQAVAVAEAGDEKLEIGLSRRVLGQ
                     AFLAQDDLAGAERELQASLRVLESVGGSLEAANTRLALAHVRWRQGQPEAARRLVAAA
                     IETYESVGAEALIARAQGS"
BASE COUNT          724 a         1218 c         1276 g          749 t
ORIGIN      
        1 gtcggtcagg tagatggcat accaatcgaa atcgcgtggg tggctgagga atgaggtgta
       61 gaccacgcgg gcgcgaatcg ggtaggcgtc ttcgggccta tcgtttggct caaagttgtc
      121 gaacccggcg ccggatggaa gtctgagcag tgtgacctgt accgcgagcg catcgttgac
      181 caggctgcgc acgacggcgc tgcctctgaa attgtgcagc gccgcatcat gcggcgggtc
      241 cgctgtacgg atgcgggccg ttccgagcag gttcaggttg gagatgatgt gcctgtagac
      301 cagggccccg tcgtcgtcga agaacgcgac ctcgaccggg ccgccctggc cggcgctggc
      361 gccgatgacc agttccgttt caatggatgc gtcgttgacg accagcggca cgtgcacgtc
      421 gccgaaagcc agacttggcc tgtcgaacca gttcacgccg acctgggcgt ttccgcgctg
      481 gttgacggtg tgtccaaacc cccacgggct gaagctgatc gtcccgataa agggatcatc
      541 ccacacggcc gtgtcgtcga cgacgagatg gcgcagtgtg cccgtcggcg gaagcgcagc
      601 ccggaactcg ctgttgagcg tgccgctggg cgagtaatag ctaagtgtga ggttttgagc
      661 gaccggcaca acgctgcgga tccacagatc ggatttgacg ccaaatggtt ggttgaacag
      721 cggcagcaaa aggaattccg atccttcaaa gaggcccggc caggcctcgg tggcgtacgt
      781 gtcaccgaag tcgttggtgt tgatcgtcaa cccgacaagg ccggcccccg tgctttcgat
      841 cagggctaca ccggagaaat tgacgagatt gggattgagc gccgaatcga tcgtgagaag
      901 ggcgttgggt gggatcaggt aaagactcgt ggcggctggc aaatagctcc ccgtctcgtc
      961 gaaataacga acgatgactg atgcgctggc gacattatcc gcgttctgga cggcgatgat
     1021 cgtgtggcga tgatccggtg acccctcgcc atcactgtcg atcggcgccc acagccggct
     1081 gaaagctgtc ccggccggaa tggcatcata ggcgaagaat gcatcgccgg gctgcgtcgc
     1141 cgagcggacc agcgcggcac cgaccaggct gccgccgccc ggaggtgcgt caagcgtggc
     1201 cgaaccgatg aagttgtcag gcaactgcgc cataaatgcc agatcgaaga tgtgtgagcc
     1261 attgccggcc agcatcgccg tcgcggtcaa ctgcaacgcg ccaccgtgaa tggtcagagt
     1321 gaccggcacc ggcaccgaca gcatgttgcg cgcagccagc atcgacgtcc aaccgtttcc
     1381 agcattgcgg gcaacgtagg ggtagtggag ccggtcgccg cccgccagcg cgtaagccgt
     1441 gtagccggcg gcccgcacgg gaggagtcag tgtgggcgca tatggggcga agtaaaggtc
     1501 atcgatggat tccgacagtg ccgtgactcc gtaatcgagc gagacgctcg caatacgccc
     1561 ctctgggtcg tagataccga tgaattccgt gtgcggctcc ggcaccgggg cattgctcac
     1621 ctggcagatg accgcattga atgcgtcgta ggcggtcatc agcccgatgg gacccagggt
     1681 gtccccgttg cccatccaga aacccacgtg agtcttgggc cggtcgaaga agatgcgcat
     1741 cgacacgccg ctgctggtgt tggggaagac cgcgttgttg gaggcaacgt ttggcggcga
     1801 ttgcgcctgc gcaggttcat tgccatagac gatggcctgc gttgtgctgt tgctctcgaa
     1861 ggtgacaccg taggcggccc ggtaatggtc gccgatgatc gccgcattgg ccagatcgtc
     1921 gaagttgatg aggcccggcg cgggaatatc cgtgggtttg aacagctcct ggcaagtctg
     1981 ctgctggctc acgagcgggg cagccgagaa acccgctccc tcggagaaac caggtttctg
     2041 gggacgactc aaggccagct cgaaggaaag ccataatgcg ctcacggcga tgacgcagat
     2101 aaagacggtg cggcggatgc gagtggacat ggtggattcc tcccagaaat ctacccttcg
     2161 gctggcctca ttccgacacg cagagaggac gtctcctccg atcacttttc aggagccttg
     2221 ggcacgtgcg atcagggctt ccgcgccgac cgactcgtaa gtctcgatgg ctgccgcgac
     2281 cagccggcgc gcggcttccg gctgcccctg acgccagcgc acatgcgcca acgccaggcg
     2341 cgtgttggct gcctctagtg aaccgccgac gctttccagt acacgcagac tggcctgcag
     2401 ctcgcgttca gcgccggcca ggtcgtcttg agccaggaat gcctgaccca gcacgcgtcg
     2461 cgataggcca atctccagct tctcatcccc agcctcggcc acggccacgg cttgctgtgc
     2521 ccggttcagc gcctcgacta cccggccctg gcccaggctg agctcggctc gatgacgaag
     2581 gagttcggct ttgaaatcct ctgaaccgat ctcggcaaac aacttctcgc tctcagccaa
     2641 gcgttcggct gcttcctccc atttgcgctc tgcgatggcg gtagccgcca agttattgtg
     2701 cagcagagct atggcatacg agttgcgtaa atcgcgccag atgcgaaggc tttgagtctg
     2761 catttgccga gcggctgccc attcaccctt ggcccggtag atctcaccaa ggttaagggt
     2821 gatcattgcc tggccagcca catcaccgat ctgttccttg atctgaagac cttgctggaa
     2881 gtagtccgtt gcttgtatca actgacctac agcctgataa gcgcttgcca gcgtgttcaa
     2941 cgcctgagat tggcccaaca tatctcctgc taataggtag acggtcgcgg ctcgctgcgc
     3001 tgactcaatc gcctgctcca gatcaccgcg tttgtagtgg atgcctgcca gcagatactg
     3061 tgcgtgcgcc tcggtgcgcc gggcggcatc gtctggcagg atgcgcgatg tcgccaggct
     3121 cttctgactc cactcggcgg cctgatcgtg ccggccctgc cgatggaata cgcccgcacc
     3181 catcaggtaa atgcgcgccg cttcgagcga gtctgccgcc gcatgctcga gtccctggtt
     3241 cagccaggtg aacgcctctt cgtactcgct gcgcttctcg tgcgtctccg ccgtcttgcg
     3301 gcataggtcg gccaagtggc gggcgtcgtt ggccgccagc gccctggctc gttcgtagct
     3361 gcgcagcgcc tcgtcgtact cgccggtgat cgagagcagg tcgcccagcg cctcgtgcgc
     3421 ggcgagctgt tccagcctgc gcggcggcga cagcctctcg gcggccagga gagcgcggcg
     3481 ataagcagca gcggcggcgg tgttggcata ctgatgctgc gcgcaccatc cggcctcaag
     3541 cgcgtagccg agcgccttgt cccacatttg accctcaaag aagtggtgcg tcaataggcc
     3601 ggatcggtca tcgccgagcg cggacgtctt ttcgagcact tcgccgatgc ggcgatgcag
     3661 ttcgcgacgg cgctcgtagc gcaggctgtc gtacgcgact tcttgagtcg tcacgctgcg
     3721 aaacgcgtag cgcgcctggc tcgagccgga ttcgaccgga agcaggccaa gatcggccag
     3781 gtcactcaaa tgccgccgaa gcgattcgag gttctcgccg ggcacgtcgt acacggcgtg
     3841 cagcagatcg agatcgacta cccgaccgac gacggcggcg acttgcagcg cgcggcgtgc
     3901 cggctccggc aggcggtcga tgcggcccat aatcacgccg tggatcgtat ccggcatttc
     3961 gatcgac
//
LOCUS       DBNHXB010000467         4313 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_824907, whole genome shotgun sequence.
ACCESSION   DBNHXB010000467 DBNHXB010000000
VERSION     DBNHXB010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_824907"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="AC162G_18185"
     CDS             <1..400
                     /locus_tag="AC162G_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077789.1"
                     /translation="VSVAQDLTGGSEPHAVFLAYLWNDLINYFGDRTTFFYRRIPANE
                     SEFEATLTRVVTDLLRSGVPVFYVEDRQPPYQNSLRILQREFKVEMWKSAPLPVYRIE
                     SSPGSGDGIGHGQRSRTVDECRRQFAGLPF"
     gene            363..1328
                     /locus_tag="AC162G_18190"
     CDS             363..1328
                     /locus_tag="AC162G_18190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5077790.1"
                     /translation="MNADDNLLDYPFDQYQRYQDVRLVVDTIQSRHQAELLTILDVGG
                     SPLTKRFLSSYAVVSVNLEAGSGVQAQCDGTRLPFADGRFDIVVTVDTLEHVPEALRA
                     VFIRELLRVSSDYAIITGPFANDYNEAAEAALNEFLVNVIGMQHRFLTEHLQNGLPDL
                     DTCLRTVTEICKGHVTIPSGYIHHWLPLMIIKHGLMRIAGGQDIVDELDRFYNYACYW
                     SDHRLPCYRWVVVASRSGDAATLTSVQQAFDPPEQSPTPDLNGVVAMWQVLRWQQVLK
                     ERNEQIDRLQTENRRLNELVSAYASGRFMRFMAALKLLIGLARRK"
     gene            1441..2583
                     /locus_tag="AC162G_18195"
     CDS             1441..2583
                     /locus_tag="AC162G_18195"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025877.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077791.1"
                     /translation="MNRRIRAKSEEFWALKDISFEIEPGETVGLIGPNGTGKSTVLKL
                     INRIIEPTSGRVSTHGRVAGLLELGTGFHPDLSGRENILLNASVLGIPRQVVKHQIDD
                     IIELADIGSFIDVPVRNYSSGMAMRLGFAITTTLEPEILLIDEILAVGDHSFQRKCLD
                     RLDALRHQGVTILFVSHSLDQVQRLCRRAIWMARGEIRADGDAESIIGMYLDAENPSE
                     VRRFLPQVSVNSDGSARWGTYQAEITAIEFLDRDGQPRSSFTSGDYFCVRMHYQTQKP
                     VSQPAFGLAIYRSDGLHLNGPNSVMGGLDVPAIDGCGYVDYAIDSLPLAPGRYELSVA
                     IYNSDLTVAHDHHHRLYSFEVEDRRLHREEGVVHIPAAWRHFADGR"
     gene            2629..3951
                     /locus_tag="AC162G_18200"
     CDS             2629..3951
                     /locus_tag="AC162G_18200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5077792.1"
                     /translation="MNAASIVIPAWNGVTWIADCLRSVGLQCRPGDEIIVVDNGSTDG
                     TPDFVARDFPQARLIRLERNLGFAGGINRGLEAARGDVLVLINQDVTLREGCLDALRR
                     RLDESGPGIVGCKLLYPDGRTIQHAGGIIRYPRAEPDHRGYRQIDDGRWDSVAQVDYA
                     SGAVFAFSRAVLRAIGPFDEGFYPAYFEEVDYCFRARAAGFAVIYEPAAVAVHHESQS
                     LDVGSLARQQAVQRNRLRFVLKSYPAEKIQTAFFPAEREHLITLPPSFAREALAPAYF
                     DALSSLPDLDGAAFLSEVNRVALSDIAAGLAELYVRARHSPAALEGAMPDHPIQLPPL
                     REHDFHSNVPMVGGLIGLARRMLYALTAKWGVWSVILQQNQINQAIVQYLIEQRSHVK
                     ELETRLIDQDRDLAQLCRTVAELEIRCRQLAKQVAHTDVPHAPEAGDN"
     gene            3960..>4313
                     /locus_tag="AC162G_18205"
     CDS             3960..>4313
                     /locus_tag="AC162G_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077793.1"
                     /translation="MRLAVVTPLDSCETGVADYSLDLLPHLAQVAGSEIAVFSEDRQA
                     IGLNGGQGWAWRSIVDLPPAADEFDLIIYQMGNSPAHDFMAPHLFRHPGLIVLHDVSL
                     HDFFARQAARYNRPSV"
BASE COUNT          838 a         1219 c         1330 g          926 t
ORIGIN      
        1 cgtatcggtg gcgcaggatt tgacaggggg gagtgaacct catgcggtct ttctcgccta
       61 tctctggaac gacctgatca actatttcgg cgatcgcacc acgttcttct accggcgaat
      121 tcctgcgaat gaatccgaat ttgaggctac cttgacgcga gttgtgactg acctgctgcg
      181 cagcggcgtg ccggtttttt acgtggaaga tagacagccc ccgtaccaaa acagcttgcg
      241 catcttgcag cgcgaattca aggttgaaat gtggaaaagt gcgccgttac ccgtgtatcg
      301 aattgaatcg tcccccggat caggcgatgg tataggacat ggacagaggt cacgcacagt
      361 tgatgaatgc agacgacaat ttgctggatt acccttttga tcaataccag cgttatcaag
      421 atgtccggct ggtcgtggat accatccagt ctcgccatca ggccgagttg ttgaccattc
      481 tggatgtggg cggctcgccg ctcacgaaga ggtttctatc cagctacgca gtcgtgtcgg
      541 taaatctcga agcgggcagc ggcgttcaag cccagtgcga tggaacgcgg ctgccatttg
      601 cggatggtcg tttcgatatt gtcgtgaccg tcgacacgct ggaacatgta ccggaagcgc
      661 tgcgtgcggt attcatccgc gaactgttac gagtgtcgag cgattatgcg atcatcaccg
      721 gcccgtttgc caacgactac aacgaagcgg ctgaggcggc tttgaacgag tttctggtga
      781 acgtcatcgg catgcagcat cggtttttga ccgagcatct tcagaacggt ctgccagacc
      841 tggacacctg tcttcgaaca gtcactgaaa tctgcaaggg tcatgtcact attccaagcg
      901 ggtacattca ccactggctg ccgctcatga tcatcaaaca cggcctgatg cgtatcgctg
      961 gcgggcagga cattgtggac gagctggatc gcttctacaa ttatgcgtgt tattggagcg
     1021 atcatcgcct gccatgttac cgctgggtcg tcgtcgcttc caggagtggt gatgcagcca
     1081 cattgaccag cgttcagcaa gcgttcgatc cgccagagca atcgcccact ccggatctga
     1141 atggagttgt ggccatgtgg caggtgttac gctggcagca agtgttgaag gaacggaacg
     1201 aacagatcga tcggctgcaa acagagaacc gccgcctgaa cgaattggtg tctgcctatg
     1261 cttcgggacg gtttatgcga ttcatggctg cactgaagct tctgatcggc ctggcccgtc
     1321 gaaagtaagc gggccgtatt gtagagtatc tattccttga ccactgccat acagtttcat
     1381 caggtctcaa agaaattcaa gttgagtccc gcccgtccgc gctcgttcat ggagatggtc
     1441 gtgaaccggc gcatccgcgc caagtccgag gagttttggg cgctgaagga catcagtttc
     1501 gagatcgagc cgggcgagac agtcgggttg atcggcccca atggcacggg caagagcacc
     1561 gtcctcaagt tgatcaaccg catcatcgaa ccgaccagcg gcagggtcag cacgcatggg
     1621 cgcgtcgccg gcctcctcga gctaggcacc gggtttcatc ccgacctgtc cggccgcgag
     1681 aatatcctgt tgaacgcttc cgtgctcggc attccgcgcc aggtggtcaa gcaccagatc
     1741 gacgacatca tcgagcttgc cgacattgga tcgttcatcg acgtgccggt gcgcaactac
     1801 tcgtcgggca tggcgatgcg gctcggcttc gcgatcacga cgacgctgga gccggaaatc
     1861 ctgttgattg acgaaatcct ggcggtgggc gatcacagtt ttcagcgcaa gtgcctggat
     1921 cgattggatg cgctgcgcca tcagggagtg acgattctat ttgtctcgca cagcctcgac
     1981 caggtgcagc gcttgtgccg gcgcgcgatc tggatggcgc gtggcgaaat ccgcgcggac
     2041 ggggatgccg agagcatcat cggtatgtac ctggatgcgg agaatccctc tgaagtgcgc
     2101 cggttcttgc ctcaagtgag cgtgaatagc gacgggagcg ctcgctgggg aacatatcaa
     2161 gccgagatca cggccattga gtttctggat cgagatggtc agcctcgctc atcatttacg
     2221 agcggagatt acttttgtgt gcgcatgcac tatcagacac aaaagccggt tagccagccg
     2281 gcctttgggt tggcgattta tcgcagcgac gggctgcatc tcaacggccc caattcggtg
     2341 atggggggac ttgacgtccc agccatcgac ggatgtggtt acgtggacta tgcgatcgac
     2401 tcgctgccgc tggcgccggg ccgctatgag ctgagcgtcg cgatctataa tagtgatttg
     2461 accgtcgcgc atgatcatca tcaccgcctg tattcgttcg aggtggagga tcgccgcctg
     2521 catcgcgaag agggcgtcgt ccacattcca gccgcctggc ggcatttcgc cgatgggcgt
     2581 tagtcatctt tccgtctggc gtggcgcgaa cgggcgaggg agatctcctt gaacgcggcc
     2641 tcgatcgtca ttccggcctg gaacggtgtg acgtggatcg ccgattgtct tcgatcggtt
     2701 gggctacagt gccggccagg cgacgagatc attgtcgtcg acaacggatc caccgatggc
     2761 acgcctgatt tcgtcgcacg ggatttcccg caggcgcgcc tcattcgcct cgagcgcaac
     2821 ctgggattcg ccggcggcat caatcgcggc ctggaagcgg cgcgcggcga tgtgctggtt
     2881 ctgattaacc aggatgtcac gctgcgcgaa ggctgcctgg atgcgctgcg ccggcggctg
     2941 gacgaaagcg ggcctggcat cgtcggatgc aagctgctgt atcccgacgg ccggacgatt
     3001 caacatgcgg gtggtatcat tcgctatcca cgcgccgaac ccgaccatcg cggctaccgg
     3061 caaatcgacg acggtcgctg ggacagcgtg gcacaggttg attacgcctc gggcgccgtg
     3121 ttcgctttca gccgcgcggt gctgcgcgca attggcccgt tcgacgaggg attctatccg
     3181 gcctatttcg aagaggttga ctactgcttt cgcgcgcggg cggctggttt cgccgtaatc
     3241 tacgaaccgg cggccgtcgc cgtgcaccac gagtcgcagt cgctggacgt gggaagcctg
     3301 gcgcgccagc aagcggtgca gcgcaaccgg ctgcgcttcg ttctgaagag ttatcccgcc
     3361 gagaagatcc aaacagcgtt ttttccagcc gagcgggagc acttgataac tctaccgccg
     3421 tcgtttgcgc gcgaagcgct ggcgccggcc tacttcgatg cgctgtcgag cttgccggac
     3481 ctcgatggcg cagcgtttct gtcagaggtc aaccgggtgg cgctaagtga cattgcggca
     3541 ggcctggccg agctgtatgt cagggccaga cattctcctg ccgcactgga gggggctatg
     3601 ccggatcatc cgatacaatt gccgccattg cgcgaacacg atttccactc gaatgtcccg
     3661 atggtgggcg gcctgatcgg tctggcgcgg cgtatgctgt atgcgctgac cgcgaagtgg
     3721 ggtgtgtggt cggtgatcct ccagcagaat cagatcaatc aggctatcgt gcagtatttg
     3781 atcgaacagc gcagccatgt gaaagaactg gaaacccgcc tgatcgatca ggatcgcgat
     3841 ctggcgcaat tgtgccgtac tgtggccgag ctcgagattc gctgccggca actggcgaag
     3901 caggtagcgc acaccgacgt cccgcacgcg cccgaagcag gggacaattg aggcgaatca
     3961 tgcgcttggc cgttgtcacc ccgttggact cgtgtgagac aggcgtcgca gactacagcc
     4021 ttgacttgtt gccacactta gcgcaggtgg cgggcagcga gatcgcggtt ttcagtgaag
     4081 acaggcaagc gatcggcctg aacggcggcc agggctgggc atggcgatcg attgtcgacc
     4141 tgccgcccgc ggccgacgag ttcgatctca tcatctatca aatgggcaac agtccagcgc
     4201 acgacttcat ggcgccacat ctgttccgcc atcccggcct gattgtcctc cacgatgtgt
     4261 cgctgcacga tttcttcgcg cgccaggcgg cccgatataa tcggcccagc gtg
//
LOCUS       DBNHXB010000468         4661 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_153142, whole genome shotgun sequence.
ACCESSION   DBNHXB010000468 DBNHXB010000000
VERSION     DBNHXB010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_153142"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /locus_tag="AC162G_18210"
     CDS             <1..1602
                     /locus_tag="AC162G_18210"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5077794.1"
                     /translation="RACFSSFGAGYVSVAAPGQLVYSTTPLDANGQDTYGTFSGTSMA
                     TPHAAGLAGLLFSQDLNRTNAQVRSLMESTAEDLGPTGVDAYFGHGRINAYRAVQGDT
                     SPTTPPAGVFSDDLTASGYAHARKLARDAGGTLHLAWHRKDGDQYQVLYAASSDDGAS
                     WSTPQVVFASSAETFHPALAVDDEYIYVVFPSKDGTTVYRTFFTRRLLTGGEWSSPAP
                     LTPSSYNAVRPDIYRDPSNGRLHVVASSFDDAPYVYYTTSDDMGASWNPLRQVNVASA
                     GGQRTRYASIHANGPNVFITGRTLEFIFLILPRFRVFAIRSTNDGETWSNLTGLAVHD
                     GLLSGEYGASLAGVGDRLYLAYEHDGGVYFRHSEGGATWSDAESLGTGAWPSITQRDD
                     GQAWLMWESDGGLFLRHYTGSTWDPTETILAATGLNKGYYPNLKLGASSDRVEWAATH
                     CSGAPFRLIVDGRAAGGTPPPTPTDTPTATNTPTPTDTSTPTPTPTNTPTPTPTATFT
                     LTPTSTIRPTATPTGYRTFLPLITK"
     gene            complement(1660..1794)
                     /locus_tag="AC162G_18215"
     CDS             complement(1660..1794)
                     /locus_tag="AC162G_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077795.1"
                     /translation="MNWLAVAYYAFRAGWSLGAVLLSAYLVWGALLGVAFVAGWLSFD
                     "
     gene            2270..3730
                     /locus_tag="AC162G_18220"
     CDS             2270..3730
                     /locus_tag="AC162G_18220"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR5077796.1"
                     /translation="MPESSPQVLAACDTDLQAQAMRAGHVPNWNALGLEACYEMTLEL
                     KPDGQSYAGTARVTFANLTGGDLPDIVFRIYPNSRIIYGGALDITSAQVDGRDAALEN
                     FLPDKTAVRLPLETALPSGAITMIDLQFEGRMPIDFASSGVYGIFNYSTEGPIATMAN
                     WYPILAVWDVERGAWSAERVLGEGDAVVSETALYRVHVSAPAGWSVVTTGSLLESKSE
                     GNSTTHDFVSGPTRDFMIVASPAFESREAEAPSGAKGQANGVRVVHWGLPKDDPSRDT
                     VLEVASGSIGAYDARIGAYPFAELDVVAVPLQNASGVEYPGLILIADDLYTSGSDRWL
                     QVTTAHEVGHQWWYSVVGSDVLESPWQDEGLTSFTSALYFEDNFPDFYRVLIDFYELG
                     FDDYKERERDEPIAQPLSAFRGRGDAYGAIVYAKGALFFADLREQMGDDAFFEALQRY
                     YAENQYAIASPETLLAAFEASCGCELDTLYAEWGVE"
     gene            complement(3771..>4661)
                     /locus_tag="AC162G_18225"
     CDS             complement(3771..>4661)
                     /locus_tag="AC162G_18225"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5077797.1"
                     /translation="TRPDVVVVSTPNHQHLPMTLAALEAGAHVICEKPLALDAAEAAA
                     MLDRAEARRRRHMTFFTYRGMAGPRYVKQLVSSRYVGRLLHAQVCYVQGSWLNPDRPA
                     SWKAIRAEAGTGVLGDLGVHAIDMLHWWFGPIARVAGSLATFIGERPGAGGGRVRVET
                     DDVASFVGEFAGGGQVSVQVSRLAAQRQNFQRFELYGSDGMLVYEYDQPLAHVGRVLG
                     ARAGEGDPKPISIPESLSEGLVGQDTFPRLYRILTDPFFASLQESQPSPSPSFADGLA
                     AQKVVDAVARSARSGKWEKV"
BASE COUNT          877 a         1555 c         1418 g          811 t
ORIGIN      
        1 cgcgcctgct tctccagttt cggcgccggc tacgtctcgg tggccgcgcc cggccagctc
       61 gtctactcga cgacgccgct ggacgccaac ggccaggata cgtacggcac cttctccggg
      121 acctcgatgg cgaccccgca cgcggccggg ctggcgggat tgttgttctc tcaggatctg
      181 aatcggacga atgcccaggt gcgcagcctg atggaatcga ccgccgagga cctggggccg
      241 accggcgtcg acgcctattt cggacacggg cggatcaatg cctatcgcgc cgtgcagggc
      301 gacacgtcgc cgacgacccc acccgccggc gtgttctccg acgatttgac ggcatccggc
      361 tacgcccacg cccgcaagct ggcccgcgac gccggcggca cgctgcatct ggcctggcac
      421 cgcaaggacg gggaccaata tcaagtacta tacgctgctt cgagcgacga tggcgcttcg
      481 tggagcacgc cccaggtagt cttcgccagc agtgcggaga ccttccaccc ggcgctggcc
      541 gtcgacgacg agtacattta tgttgtcttc cctagcaagg atggcaccac cgtctaccgc
      601 acattcttca cgcgccggct cttgaccggc ggtgaatggt cttcgcctgc tccacttacc
      661 ccaagctcat acaacgccgt gcgtccggac atctaccgcg atccctcgaa cggccggctg
      721 cacgtcgtcg cctccagctt cgacgatgcg ccctacgtct attacacgac ctcggacgac
      781 atgggcgcgt cgtggaaccc cctgcgccag gtcaacgtcg cttcggctgg tggccagcga
      841 acacgatacg ccagcattca cgccaacggc ccgaacgttt tcatcaccgg acgcacgctc
      901 gaattcatat tcctgattct tccgcgcttt cgcgtgttcg cgattcggtc gacaaacgac
      961 ggcgagacgt ggagcaatct gacgggactg gcggtccacg acggacttct cagcggagag
     1021 tacggcgcat cgctggcagg tgtcggcgat cggctttacc tggcttacga gcacgacggg
     1081 ggagtctact tccgccacag cgagggcggt gccacctgga gcgacgcaga gagccttggg
     1141 acaggggcct ggccctcgat cacccagcgc gacgacggcc aggcgtggct gatgtgggag
     1201 agcgacggcg gcctcttcct ccgtcattac accgggtcta cgtgggaccc gaccgagacg
     1261 atactggctg ccactggcct gaacaaaggc tattatccta acctgaagct gggtgcgagc
     1321 agtgaccggg tagagtgggc tgcgacccac tgcagtggag cgccattccg cctgatagtc
     1381 gacgggcgcg ctgcaggagg gaccccgcct cccacgccga ccgatacacc tacggcgact
     1441 aatacaccga cgccaactga tacgtctacg cccacgccca caccgactaa tacaccgacg
     1501 ccgaccccaa cggcaacctt tacacttact cccacatcga ccattaggcc cacggcaacg
     1561 ccgacgggct atcgaacgtt cctgccgctc atcactaaat agccgtctat attgagaagt
     1621 gttcgtccgg gtctgcaccg tccacctggg agacaggcct caatcgaacg atagccagcc
     1681 ggctacgaac gccactccca gcagcgcgcc ccacaccagg tacgccgaca acagcacggc
     1741 cccaagcgac caccctgcgc gaaacgcgta gtaggcaacc gccagccagt tcatatcgtc
     1801 tccttgattc agtcaatctg gctgaaagac ttcgcaagtc cccaaggcgc accctgctct
     1861 caaagtcgcc gaggtttttg aggcgcttcg ccggccggat gccctgcgca gacttccgaa
     1921 gtctggggtc gggcacgccc ggtagcgaac tttccagttc acgtgatagt atatactgcg
     1981 cgcgggcgcc ggccgcgttt tgtgaatcca cgaagggaca cgaagaaaca cgaatcttcg
     2041 tgagacttcg tgtgacttcg tggaagaaaa acatggaggc cacgtgtcac tccatcgatc
     2101 aacgcccaga ttgtttgccg tgctctctct ctggattctg ctcaccatgc tgtcgtcagg
     2161 cggctgctcc gtactgccga ctccccttgc agcgacacag gttgctgaac cgacgctacc
     2221 gccggtgctc ccggcttcgc ctccggccgc gacggcgaaa tcctcagcaa tgcctgagtc
     2281 cagcccccag gtgctcgccg cgtgtgatac cgatctgcag gcgcaggcca tgcgcgcagg
     2341 tcacgtcccg aattggaacg cgctcggcct ggaggcatgc tacgagatga cgctcgaact
     2401 gaaaccggat ggccaatcgt acgccggcac agcgcgcgtg accttcgcca atctcaccgg
     2461 cggcgatctg cctgacatcg tctttcgtat ctaccccaac tcgcgcatca tctacggcgg
     2521 cgcgctcgac attacctcgg cgcaggtaga cggccgagat gcggcgctcg aaaacttctt
     2581 gccggacaag accgccgtgc gcctgccgct cgaaacggcg ctgccgagcg gcgcgatcac
     2641 catgatcgat cttcaattcg agggccgaat gcctatagac ttcgcgtcga gcggcgtgta
     2701 cggcatcttc aactactcga cggaaggccc gatcgccacg atggcgaact ggtatcccat
     2761 cctggcagtg tgggatgtgg agcgtggggc atggagtgcg gagcgtgtgc tgggcgaagg
     2821 cgacgccgtc gtcagcgaga cggcgctgta tcgcgtgcac gtcagcgcgc ctgccggctg
     2881 gagcgtcgtg accaccgggt cactgctcga atccaagagc gaagggaatt cgacgacgca
     2941 cgacttcgtc agcggtccca cgcgcgactt catgatcgtg gccagcccgg cgttcgaatc
     3001 gcgcgaagcc gaagccccga gcggagcgaa gggacaggcg aatggcgtgc gcgtcgtgca
     3061 ttggggactg cccaaggacg atccgagccg ggacaccgtg ctggaagtag cgagcggatc
     3121 gatcggcgcg tacgacgcgc gcatcggcgc ctatccgttc gccgagctgg atgtcgtcgc
     3181 cgtccctctg caaaatgcgt cgggcgtcga atacccgggc ctgatcctga ttgccgatga
     3241 cctgtacacg agtggttcgg atcgctggct gcaagtcacc acggcgcacg aagtcggcca
     3301 ccagtggtgg tacagcgtcg tcggcagcga cgtgctggag tcgccctggc aggatgaggg
     3361 cctgacctct ttcacctcgg cgctgtactt cgaagacaat tttcccgact tctacagggt
     3421 gctgattgac ttctacgagc tgggcttcga cgactacaag gagcgtgagc gcgacgagcc
     3481 gatcgcgcag ccgctgtccg cctttcgcgg gcggggcgac gcctacggcg ccatcgtata
     3541 cgccaaagga gcgctgtttt ttgccgacct gcgggagcag atgggtgacg acgctttctt
     3601 cgaggcgctg cagcgttatt acgccgaaaa ccaatatgcg atcgccagcc cggagacact
     3661 gctcgcagcc ttcgaggcga gttgtggctg cgaactcgac acactgtatg ccgagtgggg
     3721 tgtagagtaa accctggcga aggttcgtaa ccgtcgccag ggtgcgtacg tcaaaccttc
     3781 tcccacttcc cgctcctggc ggagcgggcg accgcgtcga cgaccttctg agccgccagt
     3841 ccgtccgcaa agctgggcga gggagacggc tgcgactcct gcagcgacgc gaagaacgga
     3901 tcggtgagga tgcggtacag cctggggaat gtgtcctgtc ccacgagtcc ttcgctcagc
     3961 gactcaggga tagatatcgg cttcggatcg ccctcacctg cgcgcgcacc caaaacccgg
     4021 cccacgtgcg ccaggggttg atcgtactcg tacacgagca tcccatccga gccatacagc
     4081 tcaaagcgtt ggaagttttg ccgctgggcc gcgagtcgcg agacctgaac cgacacctgg
     4141 ccgccaccgg cgaactcgcc gacaaacgat gcgacatcgt ctgtctcgac gcggacacgg
     4201 ccgccgccgg cgccaggacg ctcgccgatg aacgtggcga ggctgcccgc cacacgcgcg
     4261 atcggcccga accaccaatg cagcatgtcg atggcatgca cgcccagatc gcccaggacg
     4321 ccggtacccg cctcggctcg gattgccttc cagctcgccg ggcgatcagg attcagccac
     4381 gagccttgca cgtaacacac ctgagcatga agcagccggc cgacatagcg cgaactcacg
     4441 agctgcttga cgtagcgcgg cccggccatg ccgcgatagg tgaaaaaggt catgtgccgc
     4501 cgcctccgcg cctcggcgcg atcgagcatg gccgccgcct ccgctgcgtc gagcgcgagc
     4561 ggtttctcgc agatgacgtg tgcgccggct tccagtgcgg ccagcgtcat cggcaagtgt
     4621 tgatgattgg gcgtgctgac gacgacgaca tccggccttg t
//
LOCUS       DBNHXB010000469         2809 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4682841, whole genome shotgun sequence.
ACCESSION   DBNHXB010000469 DBNHXB010000000
VERSION     DBNHXB010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4682841"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="AC162G_18230"
     CDS             complement(<1..620)
                     /locus_tag="AC162G_18230"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010592710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR5077798.1"
                     /translation="MPDTFDGRIARRVDEYIERLFVPRDSALEQGLIDAEQAGLPAIS
                     VSPSEGKLLYLIAKIAGAKRVLEIGTLGGYSTTWLARALPSGGRVVTLELEQRHADVA
                     RRNVDRTGVGERVEIRVGSAVDTLREMIERGEAPFDLIFIDADKKAYVEYLNLSMRLA
                     HPGTVILADNVIRRVMSPAPGDEDSRGIHAYNEAIAAHPRLESLILP"
     gene            766..1032
                     /locus_tag="AC162G_18235"
     CDS             766..1032
                     /locus_tag="AC162G_18235"
                     /inference="COORDINATES: protein motif:HMM:NF053451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBU_0592 family membrane protein"
                     /protein_id="HIR5077799.1"
                     /translation="MLNVKVLVDVAGWIGVVTLLAAYALVSTRRLQGDSVAYQSLNVA
                     GSALLILNSFYYGALPSVSVNLAWIGIGLYALARKRLSRPKELV"
     gene            1230..1568
                     /locus_tag="AC162G_18240"
     CDS             1230..1568
                     /locus_tag="AC162G_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR5077800.1"
                     /translation="MESKLYVGNLSYTTTEDDLRDLFAQAGTVKSVTLIKDRDTGRSR
                     GFAFVEMETQAEAQKAITQFHGAQLHERALTVNLARPREERPRGGFDDRRSDRSGGRS
                     RKGGTRRRSW"
     gene            complement(1633..2694)
                     /locus_tag="AC162G_18245"
     CDS             complement(1633..2694)
                     /locus_tag="AC162G_18245"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR5077801.1"
                     /translation="MKRLHTPSILRSAAALALGALLALGLAGGIASAREGADSPPARD
                     GLFAGNGSGDSGLSLTYAYVVTGNVPVYAHPAHAAGGQPPVRSLGAGYLWVSLANPRP
                     VSHDGQAWYLINANEYVRADNLAIYSASAFQGVALPAHPDRPFAWMVYSAQPSAKPGE
                     APAKDASLLSRYTLVTIHEEQQVGEWAWYRVGENQWIEQRSVGMVRPSARPEGVGPGE
                     KWIEVNLYEQTLAAYEGDRMVYATLVSSGLPWWKTQTGLFRMWSKVRMSKMSGRDGYA
                     DYYYLEDVPWTMYFYKTFALHGAYWHNRFGFPHSHGCVNLPPKDAQWLFDWTTPAAGA
                     ANWTMSAAENEGTWVWVHD"
BASE COUNT          509 a          905 c          873 g          522 t
ORIGIN      
        1 ggcaatatca gagactccag gcgcgggtgg gcggcgatcg cctcgttgta cgcatgaatg
       61 ccgcgcgaat cctcgtcgcc gggagcgggc gacatcacgc gccggatcac gttgtcggcc
      121 aggatgactg tgccaggatg cgcgagccgc atcgagagat tcaggtactc gacgtacgcc
      181 ttcttgtcgg cgtcgatgaa gatcaagtcg aaaggcgcct caccgcgctc gatcatctcg
      241 cgcagcgtat ccaccgccga cccgacgcgg atttcgacgc gctcgccgac gcccgtccga
      301 tcgacgttcc tacgcgcgac gtctgcgtgc ctttgctcca gctccagcgt gacgacgcgc
      361 ccgccggagg gcaacgcacg agccagccac gtcgtgctgt agccgccgag cgtgccgatc
      421 tcgagcacgc gcttcgcgcc ggcgattttc gcaatcaggt acagcagctt gccttcgctc
      481 ggggagacgc tgatcgccgg gaggccggct tgctcggcgt cgatcaggcc ctgttcgagc
      541 gcgctgtcgc gcgggacgaa caggcgctcg atgtactcgt ccactcgtct tgctatccgg
      601 ccgtcgaacg tgtcaggcat tgcgctcctc gaaggtcaca cgaggctcaa aaaggctgct
      661 atggtgacat tgtatccgag ttttgcgaat agtccaacct ctctgagcga aacacttgac
      721 ttggcggttg atctgtcata gactcttacc gggggtcatc tgagaatgct aaacgtcaag
      781 gtgctcgtcg acgtcgccgg ttggatcggc gtcgtcaccc tcctcgccgc atacgcgttg
      841 gtatccacgc ggaggctcca gggagattcg gtcgcctatc aatcgctgaa tgtggcggga
      901 agcgcgctgc tcatcctgaa ttcgttctac tatggagcgc ttccttccgt cagcgtgaac
      961 ctcgcctgga tcggcatcgg tctctatgcg ctggccagaa agaggctgag ccgtccgaag
     1021 gaactcgttt gacattctgc tgtaaaatag aggtcgcccc tgcgttcacg gataggctct
     1081 tgaggagcct tgcgcaggga cgcgcgcgaa gggagagccg ctatctcccc tctcgagcgc
     1141 gacacacgaa aggagagtga gtctgcgaag ccggtatcaa ctccgccgcc cattgagccg
     1201 agaagtgcat cgagtcagac aggagcaaaa tggaatccaa actgtatgta ggcaatctgt
     1261 cgtatacgac gacggaggat gatttgcgcg acctgttcgc gcaagccggc acggtcaagt
     1321 ccgttacctt gatcaaagac cgggatacgg gtcgctcccg gggattcgcc ttcgtggaaa
     1381 tggagacgca agccgaggcg caaaaggcga tcacccagtt tcacggtgct caactgcacg
     1441 agcgcgcgct caccgtgaac ctggctcgcc cacgcgaaga gcggccgcgg ggtgggtttg
     1501 acgaccgcag aagcgatcgt tcaggcggac gctctcgtaa aggaggcact cgccgccgct
     1561 cgtggtgacg atccggcaga ttcgccgacc gccccgaccg accagggtat ggtaggccgg
     1621 ggcggtcgct cgttaatcgt gcacccagac ccaggtgccc tcgttctccg ctgcggacat
     1681 ggtccagttt gccgctccgg ccgccggcgt cgtccagtcg aagagccact gcgcgtcctt
     1741 cggcggcagg ttcacgcacc cgtgcgaatg ggggaatccg aagcggttgt gccagtacgc
     1801 cccgtgcagc gcaaacgtct tgtagaagta cattgtccac ggcacatctt cgaggtagta
     1861 gtagtccgcg tagccgtcgc gcccgctcat cttcgacatc ctgaccttgc tccacatgcg
     1921 gaacaggccc gtctgtgtct tccaccacgg caggccggac gagacgagcg tggcgtacac
     1981 catgcgatcg ccttcgtagg ccgccagtgt ctgctcgtat agattgacct cgatccactt
     2041 ctcgcccggg ccgacgccct ccgggcgcgc agagggcctc accatgccga cgctgcgctg
     2101 ctcgatccac tgattctcgc ccacgcgata ccacgcccac tcgccgacct gctgttcctc
     2161 gtggatcgtc accagagtgt aacgcgagag gagcgacgcg tctttcgcgg gcgcttcgcc
     2221 cggcttggcc gatggctggg cgctgtacac catccaggcg aagggccggt cgggatgcgc
     2281 cggcagcgcg acgccctgga aggccgacgc cgagtaaatc gccaggttat ccgcgcgcac
     2341 gtattcattc gcgttgatca ggtaccaggc ctggccgtcg tggctgacag gtcgggggtt
     2401 agccaggctg acccagaggt agccggcgcc cagcgatcgc accgggggct ggccgccggc
     2461 agcgtgcgcc ggatgcgcgt agaccggcac gttgcccgtg accacgtagg cataagtcag
     2521 actcaggcca gaatcgccgc tcccattccc cgcgaacaga ccatcgcgcg cgggcggcga
     2581 gtcagcgcct tctcgcgctg aggcaattcc gccggccagg cccaatgcca gcagcgcgcc
     2641 gagcgccagc gcggccgcgc ttctgagaat cgatggtgtg tgaagtcgct tcatgacggc
     2701 cccctgttgc ctgcctcgct tgcgattata tccgaatatg agacgcctac aatcacccat
     2761 atgtttcaac ttcgcctact ccgaaagtcg gatgggaggc ttccacgtc
//
LOCUS       DBNHXB010000470         4006 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1831205, whole genome shotgun sequence.
ACCESSION   DBNHXB010000470 DBNHXB010000000
VERSION     DBNHXB010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1831205"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..774)
                     /locus_tag="AC162G_18250"
     CDS             complement(109..774)
                     /locus_tag="AC162G_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077802.1"
                     /translation="MNKPFCALSIFLLLLSIGTGCGSQGVSRFIITPQPTATSQPTAT
                     LIPGWEKFEGGGAELWLPESYEGGDLSSDLDFIVENLRNLGGPEFEQIAQLIEENPSA
                     FVLYVFDSKIGDSGFLTGVNVTKERVLSSVTLDTYIDMAVKQFPSEFHFVERAVVSLD
                     RYEARRLVVELTLAGTRVKEVLYVVKEDNTIWSVTFATGADEFDQRLPTFEQSVRTFA
                     VQP"
     gene            complement(796..2001)
                     /gene="xseA"
                     /locus_tag="AC162G_18255"
     CDS             complement(796..2001)
                     /gene="xseA"
                     /locus_tag="AC162G_18255"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR5077803.1"
                     /translation="MDLFGQPEAYTVSDLTRYIREMLETDYRLQDVWVQGEVSNLSRP
                     GSGHVYFSLKDAGAQIRCVIWRSAAQQMTVALREGDAIVAHGRVSVYEQQGNYQLYVD
                     AVQSAGGIGDLYRQFELLKAKLQAEGLFDAERKRPLPELPRRIGIVTSPTGAALRDML
                     NVISRRWPLLEVILAPTPVQGDDAPPQIVAALRRLYARQDLDAIIVARGGGSIEDLWA
                     FNDERVARTIVAAPVPVVCGVGHETDFTIADFVADVRAPTPSAAAELVTPNRDDYLLT
                     VDAYAARLKELLQDRLDAVRAALAMHMRALQHLSPQARVATIQERLSDLSGSLATALL
                     HTVALWRERVDGQTARLSALGPLAVLERGYAIVRHEKTGKLVRSVKQVKVGDRLKVRV
                     SDGEFGVKS"
     gene            2129..3478
                     /locus_tag="AC162G_18260"
     CDS             2129..3478
                     /locus_tag="AC162G_18260"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5077804.1"
                     /translation="MLSRSRRFWDFLTPPMVTRRCARIALLVVAATLAVACDETQLAS
                     PTLQATVPPGITPTPPIEPTPTEAVVALTVWLPESLAPGNDEEGGRLLAARLDEFDAE
                     QDEVTLNDFAKKDMGRGGLLDLLRAASPVAPAVLPDVILLSDADLAVAAREGLIQPLD
                     EMLDAESESRLFAFARTAARIDGKRMGLPLVADLHHLVYVTDSVEAPSISWTDLISDS
                     IAYPFAFADGAHVGDVVLADYTLLGGTIIDAEGQPALTLDALTRLLTLYRDARIAGVV
                     AASNLDWASDDAAWDAFQSSGAPFTAARASRYLHERALAESLRHARTPLIEGRHAPPV
                     GRSWNLAVVARDPRRQALAVDLIEHLSEREFVAAWTQGEHVLPADAGALSLWNQGDEY
                     VAFARGELSRALPPPSPAALEAVSPAFLGAIRDVLLSRASPQTAAAAAVDAVANGLR"
     gene            3527..3718
                     /locus_tag="AC162G_18265"
     CDS             3527..3718
                     /locus_tag="AC162G_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077805.1"
                     /translation="MRYRSRLSSDSDGFAGLTLALEQPEPNDAWCAGLNAAISRLDYA
                     RDTASVDALAALKDSQGCP"
     gene            complement(3723..>4006)
                     /locus_tag="AC162G_18270"
     CDS             complement(3723..>4006)
                     /locus_tag="AC162G_18270"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HIR5077806.1"
                     /translation="GFWMNGSDFAVPTSTLTGTHNVFSNPLFVDPAAGDYHLQVGSPM
                     IDRGTSAGAPSTDYDGQARPAGEGVDIGADEFVPTYATYLPVVLKAFGP"
BASE COUNT          694 a         1301 c         1244 g          767 t
ORIGIN      
        1 tcgcacgtgt gcctggccgg gatgggcgac ccttcgatga aggcggaact ggcgagccgg
       61 atggtcatat gatcccttcc ttaaggtgtg ctgaaactcg tgctgaatct acggctggac
      121 ggcgaaagtg cgaacacttt gctcaaaggt tggcaggcgc tggtcaaatt catccgcgcc
      181 cgtagcaaac gttacactcc agatggtgtt atcttcctta accacgtaaa gtacttcctt
      241 gacgcgcgtg cccgcgagtg tcaattcgac caccagccgc cttgcctcat agcggtccag
      301 cgacacgacc gcacgttcga cgaagtgaaa ctcgctgggg aattgcttaa cagccatatc
      361 gatataggtg tcgagtgtga cggaagataa tactcgctcc ttggtgacat tgacaccggt
      421 caagaatcct gagtctccga tcttcgagtc aaatacatac aggacaaatg ccgatgggtt
      481 ctcctcgatt agctgtgcga tttgctcaaa ctcagggcct cccagattgc gaagattctc
      541 cacaatgaaa tccagatcgc tgctgaggtc tccgccctcg taactttccg gcagccacag
      601 ttctgcgccg ccgccctcga acttttccca gcctgggatg agtgtggctg ttggctgcga
      661 tgttgctgtc ggctggggcg tgattatgaa tcgagaaact ccttgcgatc cgcatcctgt
      721 tcctatgctt aacagcagca ggaagatgga taacgcacag aatggcttgt tcatccttaa
      781 ctctaactcc caactctaac tcttcactcc aaactcccca tcgctcactc tcaccttcaa
      841 cctatctccc accttgacct gcttcacact ccgtacgagc tttcccgttt tctcatgtcg
      901 cacgatcgcg tacccgcgtt cgagcacggc cagcgggccg agcgcgctca gtcgcgccgt
      961 ctggccgtcg acccgctcgc gccatagcgc gacggtgtgc agcagcgccg tggcgagcga
     1021 tccgctcagg tcggagagtc gttcttgaat cgtcgctact cgcgcctgcg gcgagaggtg
     1081 ctgcagtgcg cgcatgtgca tcgccaacgc cgcgcgcacc gcgtccaggc ggtcttgcag
     1141 cagctccttc agccgcgcgg cgtaagcgtc gacggtcagc agatagtcgt cgcggttggg
     1201 cgtgaccagc tcggccgcag ccgagggcgt cggcgcgcgc acgtcggcga cgaagtcggc
     1261 gatcgtgaaa tcggtctcgt gacccacgcc gcagacgacc ggcacaggcg cggcgacgat
     1321 agtccgcgcg acgcgctcgt cgttgaaggc ccacaggtcc tcgatcgagc cgccgccgcg
     1381 cgcgacgatg atggcatcca ggtcttgcct ggcgtacagc cgcctgagcg cggcgacgat
     1441 ctgcggcggc gcgtcgtcgc cctgcaccgg cgtcggggcg aggatgactt ccagcaaggg
     1501 ccagcgccgt gagatgacgt tgagcatgtc gcggagcgcc gcgccggtcg gcgaggtgac
     1561 gatgccgatg cgcctgggca gctccggcag aggccgcttg cgctcggcgt cgaagaggcc
     1621 ctcggcttgc agcttcgctt tcagcagttc aaactggcgg tacaggtcgc ctatcccgcc
     1681 cgccgattgg actgcgtcga cgtacagttg gtaattgccc tgctgctcgt agacgctcac
     1741 ccggccgtgc gcgacgatcg catcgccctc gcggagggcg acggtcatct gctgcgccgc
     1801 gctgcgccat atcacgcatc gaatctgcgc cccggcgtcc ttcagcgaga agtacacgtg
     1861 cccgcttccc ggccggctca agttgctcac ctcgccctgc acccacacat cttgcaggcg
     1921 atagtccgtc tcgagcatct cgcgtatgta gcgcgtgaga tcggatacgg tgtaggcttc
     1981 tggctggccg aacagatcca tctgcatcgt cgggaatggt agtgcaaact ggatacagtg
     2041 tcaaatgatg actaactgat tgacaactgg taattggtaa ctgggggggc tgcaaacctg
     2101 gtgtgctata attttgagtg aggccgggtt gctatcccgg tcgcgccgtt tctgggactt
     2161 cctcacaccg ccgatggtca ctcgccgctg tgctcgcata gccctgctgg tcgtcgccgc
     2221 cacacttgcg gtggcgtgcg atgaaacgca gctcgcctct ccgacgctgc aggccaccgt
     2281 tccgccgggc atcaccccga cgccgccgat cgagcctact ccgactgagg ccgtcgtggc
     2341 cctcacagtg tggctgcctg aatcgctcgc gccagggaac gacgaagagg gtggtcgcct
     2401 gctcgccgcc cgactggacg aattcgacgc ggagcaggac gaagtgacgc tgaatgactt
     2461 cgccaagaag gatatgggcc gaggcggatt gctcgacctc ttgcgcgcag ccagcccggt
     2521 cgcgccggcc gtgctccccg acgtgatcct gctgtccgat gcagacctgg ccgtcgctgc
     2581 gcgcgaaggg ctcatacagc cgctcgacga gatgctcgac gccgaaagcg aatcccggtt
     2641 gttcgccttc gcccgcaccg ccgctcgcat cgacggcaag cgcatgggcc tgccgctcgt
     2701 cgccgacctg catcatctcg tctatgtaac cgattcggtc gaagcgccct ctatctcgtg
     2761 gacggacttg atatccgatt ccatcgcgta tcctttcgcc ttcgccgacg gcgcgcacgt
     2821 cggcgacgtc gtgctggccg actacacatt gctggggggg acgatcatcg acgcggaagg
     2881 acaacccgcg ctcacgctcg acgcgctgac ccggctgctc acactttacc gcgacgcccg
     2941 aatcgcgggc gtcgtcgctg cgtcgaatct cgactgggcg agcgacgatg cggcgtggga
     3001 cgcttttcaa tcgtccggcg cgccattcac agcggcgcgc gcgtctcgct atctacacga
     3061 gcgcgcgctc gcggaaagtc tccggcacgc gcgcacgcct ctgatcgagg gcaggcacgc
     3121 gccgccggtc ggccgctcgt ggaacctggc cgtcgtcgcg cgcgacccgc gcaggcaggc
     3181 actggccgtc gatctgatcg agcatctgag cgagcgagaa ttcgtcgccg cgtggacgca
     3241 gggcgagcac gtcctgcctg cggacgccgg ggcgctctcc ttgtggaatc aaggcgatga
     3301 gtatgtggct ttcgcgcgag gcgagttgag ccgcgctttg ccgccgccat cgccggctgc
     3361 gctcgaggcc gtcagcccgg catttctcgg cgcaatacgc gacgtgctgc tcagccgcgc
     3421 ttcgccgcag acggcggcag cggctgctgt ggatgccgtt gcgaatggcc tgcggtgatc
     3481 atcggtcagg tcaggaggag aatgggacaa aattcagcag caggtcatgc ggtatcgcag
     3541 ccgcttgagc agtgattcgg acggctttgc aggcctgaca ctcgcgctcg aacagcccga
     3601 gccgaacgac gcgtggtgcg ccgggctgaa tgccgcgatc agccggctcg actacgccag
     3661 ggacacggcg agcgtagatg ccctggcggc gttaaaagat tcgcagggtt gtccataggg
     3721 ccttacggcc cgaacgcctt cagcaccacc ggcagatatg tcgcgtatgt cggcacgaac
     3781 tcgtccgcgc cgatgtcaac gccttctccc gccgggcggg cctggccgtc gtagtcggtc
     3841 gatggcgcgc ctgcgctcgt gcctcgatcg atcatcggtg agccgacctg gagatgatag
     3901 tcgcccgccg ccggatcgac gaatagagga ttgctgaaca cgttgtgcgt gccggtcagc
     3961 gtgctcgtcg gcacagcgaa gtccgagccg ttcatccaga agccgt
//
LOCUS       DBNHXB010000471         3310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4180570, whole genome shotgun sequence.
ACCESSION   DBNHXB010000471 DBNHXB010000000
VERSION     DBNHXB010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4180570"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="AC162G_18275"
     CDS             complement(<1..533)
                     /locus_tag="AC162G_18275"
                     /inference="COORDINATES: protein motif:HMM:NF048886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkZ-related protein"
                     /protein_id="HIR5077807.1"
                     /translation="MPQKPFTLSAAKLKRLRDERYRRKPSLRLKSPDDAVCFVNELGF
                     CFFWPIQGIEMPSLWAAVAGDRPVADEHDDPGHITWNWKDQSLDKRWWYYGKLLRGKA
                     TLVSLDTLPYFYALSENFGGVDDYLEEYRAGRLSAEAKTIYEVIRDGGAMDTVRLRRE
                     ARMAAESSKARFEKALT"
     gene            complement(612..1904)
                     /locus_tag="AC162G_18280"
     CDS             complement(612..1904)
                     /locus_tag="AC162G_18280"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5077808.1"
                     /translation="MDVEQKFALLADNATFEPSEEVPSVPSVPYSIESTVNSALNLPS
                     CHTANPRPTTPLVFGDPRNVEHKDRAGAAARPHSRPTGQDPKDISNSVHMAAMPGGKR
                     LPMLKTMLTSACERDCYYCPFRAGRDFRRATFKPDEMASAVDQIVRKGIAQGLFLSSG
                     IAGGGPRTQDKLIAAVEILRQKHEFAGYVHLKLMPGAEEDQIRRAMQIANRVSINLEA
                     PNTERLARIAPHKLMIDELVRPLRIVEKIRRENGGRGPSQVTQFVVGGADESDAEILK
                     TVAYLHREVRLARAYFSAFRPLRDTPLENHRPTDPLREHRLYQADFLLRQYSFTFDDF
                     TFDDAGNLPLQADPKAAWAQAHLAHAPVDLNRATREELLRVPGLGPKSVETILAARRQ
                     GRLRDLGHLRQLNILAERAAPYILLDGKRPAHQLTLFG"
     gene            complement(2202..3185)
                     /locus_tag="AC162G_18285"
     CDS             complement(2202..3185)
                     /locus_tag="AC162G_18285"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HIR5077809.1"
                     /translation="MTDLSRHPRVSVAHLPTPVHPLPRLSAHLGGPQLWTKRDDQTGL
                     AFGGNKVRKLEFLLGEAIAHQADTLITTGAAQSNHARQTAAAAARAGLRCTLVLRGEP
                     PAEATGNVLLDHLLGADVVWAGARPTPEAVDEVAAELRARGQRPFVIPLGGSTATGAL
                     GYVAAMHEWVEQCRSGGLQFDAIVFPTSSGGTQAGMVVGARTAGYDGRIVGISVDHRA
                     GEFKQALAPLATATAQRLDMPMSFDPDEFDVRDDYLGGGYAVMGDAEREAIRLAARLE
                     GLLVDPVYTGRALAGLIDLIRTGEFATGQNVLFWHTGGTPALFAYAEQLVR"
BASE COUNT          563 a         1015 c         1098 g          634 t
ORIGIN      
        1 tccgtcaggg ccttctcgaa tcgcgctttg ctcgactcgg ccgccatgcg cgcttcgcgt
       61 cgaagtcgca cggtgtccat cgcgccgccg tcgcggatca cttcgtagat cgtcttcgct
      121 tccgccgata aacggccggc gcggtactcc tcgagatagt catccacgcc gccgaagttc
      181 tcgctgagcg cgtagaagta cggcagggta tccagtgaga cgagggtggc tttgccgcgc
      241 agcagcttgc cgtaatacca ccatcgctta tcaagtgatt gatctttcca attccaggta
      301 atgtgacccg gatcgtcgtg ctcgtcggcg acgggtcgat cgccggcgac ggcggcccac
      361 aggctcggca tttcgatgcc ctggatcggc caaaagaaac agaagcccag ctcgttcacg
      421 aagcacaccg cgtcgtccgg cgacttgagc cgcagggagg gcttgcgccg gtagcgctcg
      481 tctcgcaggc gtttgagttt cgcggctgaa agcgtgaatg gcttctgagg catccgagag
      541 cttaaggatg aaggatgagg gatgaaggat gagatgtgcc gctggacgac agaaagcttg
      601 tcctctgccg gtcatccgaa tagggtcagt tgatgcgcgg gtcgcttccc gtcgagcaag
      661 atgtaaggcg cggcccgctc ggcgagaatg tttagttggc gcaagtggcc gagatcgcgt
      721 agacggccct gccggcgcgc ggcgaggatc gtctcgacgc ttttcggccc gaggcccggg
      781 acgcgcagca gctcttcgcg cgtggcacga ttcaaatcga ccggcgcgtg cgccaggtgc
      841 gcttgcgccc acgcggcctt gggatcggct tgaagcggca ggttgcccgc atcgtcgaat
      901 gtgaagtcgt cgaaggtgaa gctgtactgg cgcagcaaaa agtcggcctg atacaggcga
      961 tgctcgcgca gcggatcggt cggcctgtgg ttttccagcg gcgtgtcacg cagcggacgg
     1021 aaggcggaga agtaggcgcg cgccaggcgc acttcgcgat gcagataagc gaccgtcttc
     1081 aggatctcgg cgtcactttc gtcggcgccg ccaacgacga actgcgtgac ctggctcggg
     1141 ccgcgcccgc cgttctcgcg ccgaatcttc tcgacgatgc gcagcggccg caccagctcg
     1201 tcaatcatca gcttgtgcgg cgcgatgcgc gccagccgct cggtgttggg cgcttcgagg
     1261 ttgatcgaca cgcggttggc gatctgcatc gcccggcgga tctgatcttc ttccgcgccc
     1321 ggcatgagct tcagatgaac gtatccggcg aattcgtgct tctggcgcag aatctcgacg
     1381 gcggcgatga gcttgtcttg cgtgcgcggc ccaccgccgg cgattccgct gctcaggaac
     1441 aatccctgtg cgatgccttt gcgcacgatc tggtcgacgg cactcgccat ctcgtcgggc
     1501 ttgaacgtgg cgcggcgaaa gtcacgtccg gcgcgaaacg ggcagtagta gcaatcgcgc
     1561 tcgcacgccg aggtgagcat cgtcttgagc atcggcaggc gcttgccgcc cggcatggcc
     1621 gccatgtgaa cgctgttcga gatgtcttta ggatcctgcc cagtcgggcg ggagtgcggc
     1681 cgcgcggcgg cgccggcgcg gtccttgtgc tcgacattgc gcggatcgcc gaacacgagc
     1741 ggggtggtgg ggcgtgggtt ggcggtgtgg catgatggca ggttgagcgc agagttaacc
     1801 gtactctcga tcgagtaggg aactgatgga actgaaggaa cttcttcgct cggctcgaat
     1861 gtggcgttgt cggccagcag tgcgaacttc tgctcgacgt ccatgcctat agtatacgaa
     1921 cttttgttct attcgtcaag tgtgtcaacg gattgcttcg cacaacggat aagcggatgg
     1981 gcgagccggg tgtggtagcc gcatctcaaa atgctgattg acgctttgcc tgtttttcgc
     2041 acctgctccc gccggatgat tcatccggcg gccgaaggcg gcggattgat cgtccgccgc
     2101 gagcgccgcc aaaagtgagc gcaaggtgcg aaatctgggc tttgtcgcaa tgatggctcg
     2161 accaaccgac tatctgacca aacgactgac tgactatccg actaccggac taactgctct
     2221 gcatacgcaa agagggctgg cgtgccgccg gtgtgccaga agagcacatt ctggccggta
     2281 gcgaactcgc ctgtgcggat cagatcgatc aggcctgcca gcgcgcggcc ggtgtacacc
     2341 ggatcgacca gcagtccttc gaggcgcgcc gcaagtcgaa tcgcttcgcg ctctgcatcg
     2401 cccatcactg cgtagccgcc tccgaggtag tcgtcgcgta cgtcgaattc gtccggatcg
     2461 aaagacatgg gcatgtccag ccgctgcgcc gttgcggtgg cgagcggcgc gagcgcctgc
     2521 ttgaactcgc cggcacgatg atcgacgctg atcccgacga tgcggccgtc gtagccggcg
     2581 gtgcgcgcgc cgacgaccat gccggcctgt gtgccgcctg agctggtcgg aaacacgatc
     2641 gcgtcgaatt ggaggccgcc gctccggcat tgctcgaccc attcgtgcat cgcggcgacg
     2701 tagcccagcg cgccggttgc cgtcgatccg ccgagcggta tgacgaacgg cctttgcccg
     2761 cgcgctcgaa gctcagcggc gacttcgtcc acggcttcgg gtgtgggccg cgcccctgcc
     2821 cacacgacgt ctgcgcccag gaggtgatcg agcaagacgt tgcccgttgc ttctgcgggc
     2881 ggctcgccgc gcaggacgag cgtgcatcgc aggcccgcgc gcgcggcggc ggcggccgtc
     2941 tgccgcgcgt ggttcgactg agcagcgccg gtcgtgatca atgtgtcggc ttgatgcgcg
     3001 atcgcctcgc cgagtaggaa ctcgagcttg cggactttgt tgccgccgaa cgccaacccg
     3061 gtctggtcgt ctcgcttcgt ccagagttga ggcccgccga ggtgcgccga caggcggggc
     3121 agggggtgta ccggcgtcgg gaggtgcgct acagacacac gtgggtgtcg agatagatcc
     3181 gtcatgatat tccctcagag cgcgttttca aacccgtaga gcgggcatcc ctatgcccgc
     3241 caagtactta aagctctcga aatcgaaggt ctactcgctc atcgcccgca agaaaatccc
     3301 gcacctcagg
//
LOCUS       DBNHXB010000472         2612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4181335, whole genome shotgun sequence.
ACCESSION   DBNHXB010000472 DBNHXB010000000
VERSION     DBNHXB010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4181335"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="AC162G_18290"
     CDS             <1..542
                     /locus_tag="AC162G_18290"
                     /inference="COORDINATES: protein motif:HMM:NF012933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR5077810.1"
                     /translation="QVRDAPVREIEKAIRVCGLSQQKAPRIKKALQHVTRERGALNLD
                     FLRDMPVDEARQWLMSIDGVGPKTAAIVLLFSLSKPAFPVDTHVHRVTGRLGLIPPRA
                     SAEKAHVILEGLVPAGLYLPFHLNVIMHGREVCHARKPQCAICVLRRHCRYYAEVVKP
                     RRFLDADEQCSGLRREAAL"
     gene            539..1141
                     /locus_tag="AC162G_18295"
     CDS             539..1141
                     /locus_tag="AC162G_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077811.1"
                     /translation="MKRKGKKHRLLIYGRVHRQRRWSMLITGLLFVGLYALPNFVDAE
                     TQRKLWPAQFDGVLLLLGIVALLIFLYKLVAPRLAYVRCTERNIRVQTPLYALTLSYR
                     RVAQTRPNQWGQLYPPEMTKRGQRRLLDGIRGESVLILDLNSWPVSRRFLRWWIPDVM
                     FSPHGDGLVLWVPDWMTLNREISDFVDRRRDAQMSRRPGV"
     gene            1188..2588
                     /locus_tag="AC162G_18300"
     CDS             1188..2588
                     /locus_tag="AC162G_18300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR5077812.1"
                     /translation="MPGSDYHTAVRRVLLQVLAINLAVAITKLVVGMLSGSIAVLADA
                     FNSLVDSSSNVIGLLGIRAAAAPPDADHPYGHRRYETLATLGIGALLLLAGWEVVENV
                     FNRLLQGGAPQVEPLGVVLLVLTMPVNLFIVWYEGRRGRVLRSDVLLADATQTRVGLW
                     TTLAALAGLLGGQIGMPWLDIVVALGITVFIVREAWRILRTTSSELSDGAAIDPKQVE
                     RVARKVPGVRYATRVRSRGREDDVHLDLHVKVDAAMSTAQAHAIASEVERRLKLDLPG
                     VVDAVVHIEPGQMPAPTRGEAIAVEVRAIADGLGLGVHNLHLHSVEQGYTLDADVEVD
                     AALSLEASHVQVTEFENRVRAALEDIVEVVTHIEPGQAAGAEEAAEDEEAERVRAQIV
                     AVGDRVCGRGAAHHITLRRLDGRYDVSLHVIAPGESSVVEAHLLAEEVERQLREAIAG
                     LERVTVHVEPPEGRDD"
BASE COUNT          433 a          809 c          920 g          450 t
ORIGIN      
        1 agcaggtgcg cgacgcgccc gtgcgcgaga tcgagaaggc gattcgcgtg tgcgggcttt
       61 cgcagcagaa agcaccgcgc atcaagaaag cgctgcagca cgtcacgcgc gagcgcggcg
      121 cgctgaacct cgacttcttg cgcgacatgc cggtcgacga ggcgcggcag tggctgatgt
      181 cgatcgacgg cgtcggcccg aagaccgcgg cgatcgtcct gctgttctca ttgagcaagc
      241 cggccttccc ggtcgacacg cacgtgcatc gcgtgacggg acggctgggt ctgatcccgc
      301 ccagggccag cgccgaaaag gcgcacgtga ttctcgaggg actcgtgcca gccgggctgt
      361 acttgccgtt tcacctgaac gtgatcatgc acggacggga agtgtgccac gcgcgcaagc
      421 cgcaatgtgc aatctgcgtg ttgagacgcc actgtcgcta ctatgcggag gtcgtcaaac
      481 cgcgaagatt tttggacgcg gatgaacaat gctctgggtt gcggcgggag gccgcgttat
      541 gaagcgcaag ggcaagaagc accgcctgct catctacggg cgcgtgcacc gccagcgacg
      601 ctggtcgatg ctcatcaccg ggttattgtt cgttggcctg tacgcactcc ccaacttcgt
      661 cgacgccgag acacagcgca agttgtggcc tgcgcagttc gatggcgtgc tcctgttgct
      721 cggcatcgtg gctctgctga ttttccttta caagctcgtc gcgcctcgac tcgcttacgt
      781 gcggtgcacc gagcgcaaca ttcgagtgca aacgccgctg tacgcactca ccctctcgta
      841 caggcgcgtc gcgcagacgc ggcccaacca gtgggggcag ttatatccgc cggaaatgac
      901 caaacgcggg cagcgccgcc tgctcgatgg catccggggg gagagcgtgc tgatcctcga
      961 tctcaacagc tggcctgtct cgcgcaggtt cctgagatgg tggatacctg acgtgatgtt
     1021 ctcgccccat ggcgatggct tggtgctgtg ggtaccggac tggatgacgc tcaatcgcga
     1081 aatcagcgac ttcgtcgatc gccgccgcga tgcgcagatg agccggcggc caggggtata
     1141 ggacgcagat agacgcggat gaacgcagat aggtctggat gatcagcatg cctggctcag
     1201 actaccacac cgccgtgcgc cgcgtgctgc tccaggtgtt ggcgatcaac ctggccgtgg
     1261 cgatcacgaa gctggtcgtc ggcatgctca gcggatcgat cgcggtgctg gccgacgcct
     1321 tcaactcgct ggtcgattcg tcgtcgaacg tgatcggtct gctcggcatt cgcgccgcgg
     1381 ccgcgccgcc cgacgccgat cacccgtacg gccaccgccg ctacgagacg ctggccacgc
     1441 tcggcatcgg cgcgctgctg ctcctggccg gctgggaggt ggtggagaac gtgttcaacc
     1501 gcctcctgca aggcggcgcg cctcaggtcg agccgctcgg cgtggtgctg ctcgtcctga
     1561 cgatgccggt gaacctattc atcgtgtggt acgagggccg gcgcgggcgc gtgctgcgca
     1621 gcgacgtgct gctggctgat gcgacccaga cgcgcgtcgg gttgtggacg acgctggccg
     1681 cgctggccgg cttgctcggc gggcagatcg gcatgccctg gctcgacatc gtcgtggcgc
     1741 tggggatcac ggtgttcatc gtgcgggagg catggcgcat cctgcgcacc acgtcgtcgg
     1801 agctgagcga cggggcggcg atcgatccga agcaggtcga gcgcgtggcg cggaaggtgc
     1861 cgggcgtgcg ttacgccacg cgggttcggt cgcgcggacg cgaagacgac gttcacctcg
     1921 acctgcacgt gaaggtcgac gcggcgatgt cgacggcgca ggcgcacgcc atcgcgagcg
     1981 aagtcgagcg ccgcctcaag ctcgacctgc ccggcgtcgt cgacgccgtc gttcacatcg
     2041 agccggggca aatgcccgcg ccgacgcgcg gcgaggcgat tgccgtggag gtgcgtgcga
     2101 tcgccgacgg gttggggttg ggcgtgcaca atctgcacct gcactccgtg gagcagggat
     2161 acacgctgga cgcggacgtc gaagtcgacg cggcactgtc cctggaagcg tcgcacgttc
     2221 aggtgaccga attcgagaat cgcgtgcgcg ctgcgctgga ggatatcgtc gaagtcgtca
     2281 cgcacatcga gccgggacaa gcggccggcg ccgaagaggc cgccgaggac gaggaggccg
     2341 agcgggtacg ggcgcagatc gtcgccgttg gcgatcgggt ctgcgggcgc ggcgcggcgc
     2401 atcacatcac gctgcgtcgc ctggacggcc gttacgacgt ttcactgcac gtcatcgcgc
     2461 ccggcgagtc gtccgtcgtc gaggcgcatt tactcgccga agaagtcgag cggcagttgc
     2521 gcgaagcgat cgccggcctg gagcgggtca cggtacacgt cgagccgccg gaaggtcggg
     2581 acgattgaga tatgagagtt agagttagag tt
//
LOCUS       DBNHXB010000473        13598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4684419, whole genome shotgun sequence.
ACCESSION   DBNHXB010000473 DBNHXB010000000
VERSION     DBNHXB010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 13598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13598
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4684419"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="AC162G_18305"
     CDS             complement(<1..1316)
                     /locus_tag="AC162G_18305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR5077813.1"
                     /translation="MKHLARRMVIGFLCAAVSVMIAYLALSADLPSPDALLTRASSGT
                     TKILDRNGKLLYELIDPRSGTRTRLALDDIPLYCRQATIATEDAGFYTNPGVDVFAVL
                     RAALQNAQAGEVVSGGSTITQQVARLLLLSPEERAQRSLIRKAREGILAVRLAQAYGK
                     DEILALYLNEVYYGNLAYGLEAAAQSYFGVSARDLDLAQCALLAGLPQMPARLNPLID
                     LEAARARQKVVLDLMAEQGYITHQQADLAAVEPLAFRSNDPLLAPHFVAYVRNQLEAE
                     VGADAVLTGEWVITTTLDLDLQREAEAAVTRHLADLREHDVTNAAVIVLDPDTGEILT
                     MVGSADYSDTSIDGAFNAALALRQPGSAMKPILYALAFERGASPGDVVYDVPTAFVDE
                     SGETYIPANYDNAFHGPVSLRRALANSFNLPAVILQKRVGTADFLER"
     gene            complement(1512..6320)
                     /locus_tag="AC162G_18310"
     CDS             complement(1512..6320)
                     /locus_tag="AC162G_18310"
                     /inference="COORDINATES: protein motif:HMM:NF019298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HIR5077814.1"
                     /translation="MRRPLLFRLALLALLVSAAVLLISTDYYTNLPDRLSQHETIVLG
                     QNRFVPGSQAVLRVVVRDSRNAAPLSNAGIKVALQRSDGGATIPLFTGTTDRQGTADV
                     TFQVPADVEGGHALVVETASSLGIDRIEQSVSLKRDYRILLSTDKPLYQPGQVIHVRA
                     LALSPFDLKPAAGRELNITIVDGKGNQVFRKTLTTSEYGVASTDFRLASEVNTGAYQI
                     SAELGETSSEKTVTVERYTLPKFEIELKTERAFYQPGERVRGMLHAGYFFGKPVADSA
                     VHLEGYIFDVERTVTFNLKGTTDAEGNFAFEFDLPAYLAASDLDGGLGRYYVQTTITD
                     QAQQTEVVNLSFPISDSPLVIEAVPESGQFRPGVENILYILTSTPDGQPVETSLTLTL
                     HETGQTLAAQTDAYGLAEVRLTPSSTQQFITIRANAANGTSVSRDFQFEAGPSGETVL
                     LRPDHPAYRVGETMHLTILTSQSTGNAYLDIVRDGQTISTRSVKVLDGRAEVAVDLGP
                     DLFGTLELHAYNILRSGSIVRDTRLVLVDDASDLSLTLAPDRNVYRPGETAGLDIQVK
                     GTDGNGAQSAIGLAIVDEAVFAFVEQDPGFARLYFLLEQELLQSRYDLHGFSTARLVD
                     DELASDPALRQAQENAARAALADVARHTGDFSLHANSHEQTIQRASEIQAAYFTDLSK
                     GLYLLALILPLPIGVLSIQSLERQKHLGRSFGLTLLFGVLLCPLIALSGLRGGSDLPA
                     LLGLFGVLGGLALFVKAWQRNDPSLKWMLALLLVYVVALIGLGEATSHTRFFPGYDVN
                     VLAVLGLIGVVLAFLMQLAGLMFGADAANAINTLLVLLLVGAIVASGCAPAALPTAAP
                     QLAAATEAPATVETDSATKSTAEPPRLRQYFPETMLWLPDAVTDENGQLHLDVPIADS
                     ITTWRMTALASTRDGRLGSATGGLRVFQDFFIDLNLPSALTVGDEVSLPVGVFNYLPE
                     PQSVRLVVEPAGWFELLDDAEKTIVIQANDVSVTYFRIRAAAFGTHAFKVTAWGSQMS
                     DAIQKNVDVFPDGKQVFFTQSNRLAAGDHITRSIVIPPEAIPGTQSITVKIYPGVLSQ
                     VVEGLDSILRMPNGCFEQTSSTTYPNVLVLDYLRAADQIAPETQLKAEEYINLGYQRL
                     TTFEVDGGGFSLFGEHPADRMLTAYGLQEFGDMGRVHSVDPALVARAAGWLFSQQQSD
                     GSWANDRGLVHEDTWVKLGDDRLPVTAYVVWSMIDAGFGDDPRTQRGLAYLREFENQV
                     EDAYVMALVANALVAADVQLSDSLDAATEAVLARLAEQAVREGDHVSWSSGIATFMGS
                     RGQTGSIETTALATLAFLRAGTYPDLANAALATLIQQKDSFGTWYSTQATVLALKALI
                     QSTRASGENENATVRVTLDGQTRLIQITPENAGVVQLLTFNDVGIGGASTLEISATGQ
                     GHLMYQVSGSYYVPWKDAPRFDEPGEDGEGVSIDVAYDRTELSVDDTVTVNVTVGLNQ
                     PGRAESVLIDLGLPPGFSVEAEDLAARVARDNDVPADYNGSTIERYELTGRQLLVYIR
                     NLSQGSPLSFSYRLRARFPLAAQTPASLAYDYYNPDISGEVTPQRLVVAP"
     gene            6586..7908
                     /locus_tag="AC162G_18315"
     CDS             6586..7908
                     /locus_tag="AC162G_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015171555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HIR5077815.1"
                     /translation="MSTTWTPDTILALAPEAKSAKSGRDLATLRKWVTLGCHEQAVWG
                     ECQGSAESPYQTQIDLAEPAFHCSCPSRKFPCKHALGLFLLFESQPAAFKSSAPPEWV
                     AQWLEGRAKRAKQRTGKTAQSADEVADPKAQTKRAAERQSKVAAGLQELDTWLQDVIR
                     GGIALVQNRPASFWKTPAARMVDAQAPGLARLVREMSGIPASGEGWQERLLNRVGSLH
                     LIIEGYKRIETLPAGTQADIRALIGWAQDQDEVLAGETVRDRWLVLGRRVDEEERLRV
                     QRTWLWGCESRRPALILHFAHNSQPLDMSVVPGTGLEASIVFFPGAYPLRVLIKDRLT
                     APTPLESLPGYRTIADAVAAYANALSLNPWLERFPITLESIVPVHRDERWWMRDPQGH
                     VLPLASRFMKGWHLAALSGGHPMTIFGEWDGDDLLPLSAWSDNRFVQL"
     gene            7921..9417
                     /locus_tag="AC162G_18320"
     CDS             7921..9417
                     /locus_tag="AC162G_18320"
                     /inference="COORDINATES: protein motif:HMM:NF039502.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HIR5077816.1"
                     /translation="MTAWQDLIETALLGTERRPFKVTPQGSPLDDILSRLDSSNDERA
                     PRSVVAAMSIYQRVGRLPVTDSQPVPAPCALDDAPRCSPRAGQHLALLLDGQHAQVLS
                     EWLAALGLTQKRVPEESLPALLDLGKAQPDVREAILPVLGERGLWLAAQSPDWDYALG
                     GDDAAAWQTGSLPIRLMLLQKLRRSDTTRARELMASTWPTDAPDERTAFLAACAINLG
                     TDDEPFLEAALDDRRKEVRRMAADLLAQLPESRLCGRMLDRLRPLLTLTRGLKPNIEV
                     TLPAECDKGMLRDGVEPKPPSGMGEKAWWLQQILGAVPPSIWSQTWRTSPKDLVQAAG
                     RGEWRMMLLQGWAVAAMRQRDTEWIEALLADFLAHNELAQAQPAFEGLDDSRKEAFVL
                     SLLHSNPSLQPMQLAYHFLLRCRHPWSVALSRAVLDSLQRHTSNGDIGPQFGWSPFLL
                     EVARYMNPELIAEAESRLHEAAVSGIDAIDNFLNLLRFRYDMLKEIQP"
     gene            9414..10496
                     /locus_tag="AC162G_18325"
     CDS             9414..10496
                     /locus_tag="AC162G_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015122270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5077817.1"
                     /translation="MTNLLREHAEQQFAEELHELAKVDTRPRPPNWKLSPWAVAMYVM
                     GGKLESGYVVSPKYIGSRRLIEIAIATLATDRALLLLGVPGTAKSWVSEHLAVAISGH
                     STLMVQGTAGASEEAIRYGWNYARLLADGPSMQALVPSPIMRAMQQGQLARVEELTRI
                     PSDVQDSLITVLSEKTLPIPELNTEVQAIKGFNVIATANNRDRGVNELSSALKRRFNT
                     VVLPVPDSVEQEVDIVQRRVEALGRALELPAETPALEEIRRIVTVFRELRDGKSIDGK
                     TKLKSPSGTLSTAEAISVVNSGLALAAHFGDGRMRANDVASGLVGAVVKDPVQDRVVW
                     LEYLETIVKEREGWKDLYRACRGVTE"
     gene            10557..12830
                     /locus_tag="AC162G_18330"
     CDS             10557..12830
                     /locus_tag="AC162G_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HIR5077818.1"
                     /translation="MYRSTTVHIFGIRHHGPGSARSLRRALEDLKPDAILLEGPPDAE
                     AVLPLLLQRGMQPPVALFIYVPGQPQRAAFYPFAVFSPEWQALHYGLSHNIAVRFMDL
                     PQAHQLALKEEASQTPTPEPQSPVRNPQSEMSLDPLRWLAEAAGHNDGEEWWERMVEQ
                     RRDGTDLFAAILEAMTALRESVPEPPDPIGPQREAYMRQTIRAAQRQGFERIAVVCGA
                     WHVPALTPEAIAQAKDDKALLSGLPKVKVEATWTPWTYSRLLYFSGYGAGVVSPGWYH
                     HLWTTPDQVTVRWMARVARLLREADLDVSPAHLIEAVRLAESLAALRNRALPGLSELN
                     EATQTVVCFGNDTPMRLIHDRLIVGERLGTVPSDTPMPPLQRDLAHAQKRQRLPVDAA
                     QRALDLDLRKPTDLDRSLLLHRLNLLGVRWGVVQRASTGKGTFHEVWQLQWKPELAVN
                     VIEASVWGNTVVDAATTYARHLADQTAEAPEPLPALTHLVDQVLLADLPDALPHVMGR
                     LQAESAVASDIAHLMNALPPLANVMRYRDVRQTDMGMVSQVVDGLVARMCVGLPGACA
                     SLNDEAAAAMFDRLIGVDSAMALLRDDGYRAMWQAVVAHLADQHGVHGLVAGRCCRLL
                     LDQGRFVGSDVARRMSLALSPANEAEQAAAWLEGFLKGSGLLLLHDEVLWQVLDDWVK
                     ALPGETFTPLLPLLRRTFSTFTAVERRRIGERASRGSVSRLEGGGRSEEASFDSARAA
                     KVLPLLAQLLGVNTDEH"
     gene            12820..>13598
                     /locus_tag="AC162G_18335"
     CDS             12820..>13598
                     /locus_tag="AC162G_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR5077819.1"
                     /translation="MNTETERLRRWRLILGTEAAEAIPLALSGDDIVIDRALGALYGA
                     GGLSRGDGEDRRGGLEGSAPRVARWLGDIRTYFPSSVVRVMQQDALERLNLRQMLLQP
                     EMMEAVQPDVHLVATLLSLSSVMSNKAKDTARLVVRRVVEEVERRLANALRQAVTGSL
                     DRAARNRRPRYSEIDWHRTIRANLKHYQPDYHSIIPETRMGYGRKRAALRDIILCIDE
                     SGSMATSVVYAGIFGAVLASLKAIQTRLVAFDTSVVDLSQEL"
BASE COUNT         2514 a         4092 c         4446 g         2546 t
ORIGIN      
        1 cgttcgagga agtcggccgt gccgacgcgc ttctgaagga tgacggcggg caggttgaac
       61 gagttggcga gcgcgcgccg caggctgacc gggccatgaa aggcgttgtc gtagttggcc
      121 gggatgtacg tctcgcctga ctcgtcgacg aaagccgtcg gcacgtcgta caccacgtcg
      181 cccggcgatg cgcctcgctc gaaggcgagc gcgtacagga tcggtttcat cgccgagccg
      241 ggttggcgca gcgccagtgc ggcgttgaat gcgccgtcga tcgacgtgtc actgtaatcg
      301 gcgctgccga ccatcgtcag gatttcgccc gtatccggat cgagcacgat gacggcggca
      361 ttcgtcacat cgtgctcgcg cagatcggcc aggtggcgtg tgacggcggc ttctgcttca
      421 cgctgaaggt cgagatcgag cgtcgtagtg atgacccatt caccagtgag cacggcgtcc
      481 gctccgactt cggcttcgag ctgattgcgg acgtaggcga cgaagtgcgg ggcaagcagt
      541 gggtcgtttg atctgaaggc cagcggctcg acggcagcaa ggtcggcttg ctggtgcgtg
      601 atgtagccct gctcggccat caggtcgagc accaccttct ggcgggcacg ggcagcctcc
      661 agatcgatca gcgggttgag gcgggcgggc atttgcggca ggccagccag cagcgcgcac
      721 tgcgctaaat cgaggtcgcg cgccgagacg ccgaagtaag actgagccgc cgcttcgagg
      781 ccgtaagcca ggttgccgta gtagacctcg ttcaagtaga gcgcgaggat ctcgtctttg
      841 ccgtaggcct gcgcgaggcg gacggcgagg ataccttcgc gcgctttgcg gatcaggctg
      901 cgctgagcgc gttcttcggg cgaaaggagc aagaggcggg cgacctgttg ggtgatggtg
      961 ctgccgcccg agacgacttc gccggcctgc gcgttctgca gagcggcgcg cagcacggcg
     1021 aacacatcga cgccgggatt ggtgtagaag ccggcatctt cggtggcgat ggtggcctgt
     1081 cggcagtaga gcgggatgtc gtcgagcgcc agccgggtgc gcgtgccgga gcgcggatcg
     1141 ataagctcat acagcagttt gccattgcga tctaagatct tggtcgtgcc ggaagaggcg
     1201 cgcgtaagga gtgcgtcggg cgagggcaaa tcggcggata aggcgaggta agcgatcatt
     1261 acgctcacgg ccgcacacaa aaacccgatc accattcgtc gtgcgaggtg tttcatatcg
     1321 cgctcacatc tgcgtcactg tattggatca tacgcgcgtg tgttgctcct cactcgacgc
     1381 cagttctacg gcaatcgggt tgccgcccta cgatggatcg gaaaaggaga caccctcgtc
     1441 aacatatgaa gcgtccccga ccgcctggac gatcggggac gtggccttcg tccacaagtc
     1501 tggtagcagc ttcacggcgc caccaccagc ctctgcggcg tgacctcacc ggaaatgtcc
     1561 gggttgtagt aatcgtaggc gaggctggcc ggcgtctgcg ccgccagcgg gaacctggcc
     1621 cttagccgat aactgaagct caagggacta ccctggctga gattgcgaat gtagacgagc
     1681 agttgacggc cggtcagttc gtatcgctcg attgtcgacc cgttgtagtc ggcgggcaca
     1741 tcgttgtcgc gcgccacgcg cgcagccagg tcttcggcct ccacactgaa gcctggcggc
     1801 agacccaggt cgatcagcac ggactccgcc cgaccgggct ggttgaggcc gactgtcacg
     1861 ttgaccgtca ccgtatcgtc cacgctcagc tccgttcggt cgtaggccac gtcgatggat
     1921 acgccttcac cgtcttcacc aggctcgtcg aatcgaggcg catccttcca gggcacatag
     1981 tagctgcccg acacctgata catcaagtgg ccctggccag tcgcgctgat ttcgagtgtg
     2041 ctggccccgc caatcccaac gtcgttgaac gtcagcaact gtacgacacc ggcgttctca
     2101 ggtgtgatct ggatgagtcg cgtctgtccg tcgagcgtca cgcggacagt agcattctcg
     2161 ttctcgccac tggcgcgcgt gctctggatg agtgctttga gcgccagcac ggtggcctgc
     2221 gtgctgtacc acgtgccgaa gctatccttt tgttgaatca gcgtcgccag cgcagcgttt
     2281 gccagatcgg gatacgtgcc ggcgcgcagg aaggcgagcg ttgccagcgc ggtcgtttcg
     2341 atgctgccgg tttgaccccg gctgcccatg aacgtggcga tgccgctcga ccagctcacg
     2401 tgatcgccct cgcgcacagc ctgctcggct agccgggcga gcacggcttc ggtcgccgcg
     2461 tcgagcgagt cactcaactg gacatcggcg gcgaccagcg cgttcgccac aagcgccatc
     2521 acgtaagcat cttcgacctg gttctcaaac tcgcgaaggt acgctagccc tcgctgcgtg
     2581 cgcgggtcgt cgccaaaccc ggcgtcgatc atgctccaca cgacgtaggc ggtgacgggc
     2641 aggcgatcgt cgcccagctt gacccaggtg tcctcatgca ccagtcctct gtcgttggcc
     2701 cacgagccgt cgctttgttg ctgcgagaac agccacccgg ccgcgcgagc cacgagagcg
     2761 ggatcgacac tatgcacgcg gcccatgtcg ccgaactcct gcaggccata cgctgtgagc
     2821 atccggtcgg cagggtgctc gccgaacagc gagaaaccgc cgccgtccac ttcgaacgtc
     2881 gtcaggcgct ggtagcccag gttgatgtac tcctcggctt tgagttgcgt ttcgggcgcg
     2941 atctggtcgg cggcgcgtag ataatcgagc accagcacat tcggataggt ggtcgacgag
     3001 gtctgctcga agcagccatt cggcatgcgc aggatgctgt ccagcccttc gacgacctgg
     3061 ctgagcacgc cagggtagat cttcacagtg attgactgcg tgccggggat agcctcgggc
     3121 gggatgacga tggaccgggt gatgtggtca ccggcggcca gccgattaga ctgggtgaag
     3181 aagacttgtt tgccgtccgg gaagacgtcc acgtttttct ggatcgcatc ggacatctgg
     3241 ctgccccagg ccgtgacttt gaaggcgtgc gtgccgaagg ccgccgcccg gatgcggaag
     3301 taagtgacgc ttacgtcgtt cgcctggata acgatcgttt tctccgcatc gtcgagcagc
     3361 tcgaaccagc cagccggctc gacgacgaga cgcacgcttt gcggttcggg caggtagttg
     3421 aacacaccga ccggcagcga tacttcgtca ccaacggtca gcgccgacgg caggttcaag
     3481 tcgatgaaga aatcctgaaa gacgcgcagc ccgccggttg cgctacccag ccggccgtcg
     3541 cgcgtggagg cgagcgccgt catgcgccag gtggtgatgc tgtcggcgat gggaacgtcc
     3601 aggtgcaact ggccgttctc atcggtcacc gcatcgggca gccacagcat cgtttcggga
     3661 aagtattggc gtaagcgtgg cggctcggcg gtgcttttcg tcgcgctgtc tgtctcaacg
     3721 gtggcgggcg cttcggttgc agccgcgagc tgtggcgcgg ctgtgggcag ggcagcgggc
     3781 gcgcagccgc ttgccactat cgcgccgacc agcaggagca cgagtaacgt gttgatcgca
     3841 ttcgcagcgt ctgcgccaaa catcaggccg gccagttgca tgaggaaggc cagcacgacc
     3901 ccgatcaagc ccagcacggc gagaacgttg acgtcgtagc cggggaagaa gcgcgtatgc
     3961 gacgtggctt cgcccaggcc gatcaacgcg acaacgtaca ctaacagcag ggcgagcatc
     4021 cacttgagcg acgggtcgtt gcgttgccag gctttgacga acagggcaag cccaccgagc
     4081 actccaaaca gcccgagcag cgctggcagg tccgatccgc ctcgcaaccc cgacagcgcg
     4141 atcagagggc agagcagcac gccgaacaac agcgtcaacc caaagctgcg tcccaggtgc
     4201 ttttgccgct ccagcgattg gatgctcaac acaccgatcg gcagcggcag gatcagcgcg
     4261 agcagataca gacccttgct cagatcggtg aagtaggcgg cctggatctc gctggcgcgc
     4321 tggatggtct gctcatgcga gttggcgtga agactgaaat cgccggtgtg ccgtgccaca
     4381 tcggccaatg cagcacgtgc cgcgttctcc tgcgcctggc ggagtgccgg gtcgcttgcc
     4441 agctcgtcgt cgacgaggcg ggctgtgctg aaaccgtgca ggtcgtatct ggactggagc
     4501 agctcttgtt cgagcaagaa gtacagcctg gcgaagccag ggtcttgttc gacgaacgca
     4561 aacaccgctt cgtcgacgat cgccaggccg atggccgact gtgcaccatt cccgtctgtg
     4621 cccttcacct ggatgtccaa cccggccgtt tcgcctgggc ggtacacatt gcggtcgggt
     4681 gcgagcgtga ggcttaaatc gcttgcgtcg tcgaccagca ccaggcgcgt gtctcgaaca
     4741 atgctgcctg aacgcaggat attgtaggcg tgcaattcga gtgttccaaa tagatcgggc
     4801 ccgaggtcga ccgccacttc ggcacggcca tctaatacct tcaccgagcg cgtgctgatc
     4861 gtctgtccat cgcgcacaat gtcgaggtat gcgttgccgg tcgactgaga tgtcaggatg
     4921 gtcaaatgca tcgtctcgcc cacgcgataa gcaggatggt cgggccggag caggacggtt
     4981 tcgcccgaag gcccggcctc aaactggaag tcgcgcgaga cgcttgtgcc gtttgcggca
     5041 ttggcccgga tggtgatgaa ctgctgggtg ctggaaggcg tcaggcgcac ttcggccaga
     5101 ccatacgcat cggtctgtgc agccagtgtt tgacccgttt cgtgaagggt cagggtcagg
     5161 ctcgtttcga caggctggcc gtcgggtgtg ctggtcagga tgtagaggat attctcgaca
     5221 ccgggccgga attggccgct ctccggcaca gcttcgatca ccagtggact gtcggatatc
     5281 gggaaggaga ggttgacgac ttctgtctgt tgtgcctgat cggtgatcgt cgtctgcacg
     5341 taataccggc ccaggccgcc gtccaggtcg ctggctgcga ggtacgccgg caaatcgaac
     5401 tcgaaagcga agttgccttc ggcgtcggtc gtacctttca agttgaaagt gaccgtccgc
     5461 tcgacgtcga agatgtagcc ttccaagtgc accgcgctat cggcgaccgg cttgccgaag
     5521 aagtaaccgg catgcaacat cccgcgcacg cgctcgcctg gctggtagaa ggcgcgctcg
     5581 gtcttcagct cgatctcgaa cttgggcagc gtgtaacgct cgacggtaac ggtcttctcg
     5641 gacgaagttt cacccaactc ggcgctgatc tggtacgcgc cggtattgac ttcgctcgcc
     5701 aaccgaaagt cggtcgaagc gacgccgtac tcggacgtgg tgagcgtttt gcggaagact
     5761 tggttgccct tgccatcgac gatcgtgatg ttcaattccc gcccggcggc cggcttcagg
     5821 tcgaatgggc tgagcgcgag cgcgcgcacg tgaatcacct ggccgggctg atacagcggc
     5881 ttgtcggtcg acaataggat gcggtagtcg cgcttgaggc tgacggattg ctcgatgcga
     5941 tcgattccca gcgatgaagc cgtttccacg accagtgcgt ggccgccttc gacgtcggcg
     6001 ggcacctgaa aagtgacgtc ggcagtgccc tggcggtcgg tcgtgccagt aaacaacggg
     6061 attgtcgctc caccgtcact gcgttgcagc gcgactttga tgccggcgtt cgacagcggc
     6121 gcggcattgc ggctatcacg caccacgacg cgtagtacgg cttggctgcc gggcacgaag
     6181 cgattttggc ctaacacgat ggtttcgtgc tggctgaggc gatccggcag attggtgtag
     6241 tagtcggtac tgatcaacag cactgcggcc gagacgagca aggcgagcag agcgagacgg
     6301 aagagcaacg ggcggcgcat ggaaaacctc ctcgtgaagc gggcgtggat attcgatttg
     6361 cacttaagac gcgatggaag tgaaccgtgt tccaggagga ggaagtacga agcgctccat
     6421 cgttgatacc gtttcatacg ctttcaatct actcgctcat gggatctgtg atcgtagctg
     6481 tcacatgccc aagatataag agacgtagtt caattgaaca tcgcatcgtt gccgtattga
     6541 acgccatcgc tgacactcaa cttgcgctgg caacgagata tggacatgtc caccacctgg
     6601 acccctgaca caatcctggc gctcgcgccc gaagccaaat cggcgaagtc aggcagagac
     6661 ctggcaacgc tccgcaagtg ggtgacactg ggctgccatg aacaggccgt gtggggtgag
     6721 tgccagggca gcgccgaatc gccttaccaa acccagatcg accttgccga gccggcgttc
     6781 cattgctcgt gccccagccg taagttcccg tgtaagcatg cgctggggct ttttcttttg
     6841 tttgagagcc agcccgccgc gttcaagtcg agtgcgccgc cagagtgggt ggcacagtgg
     6901 ctggaaggtc gggccaaacg cgcgaagcag cgtacgggga agactgctca gtccgccgat
     6961 gaggtggccg atccgaaggc acagacgaaa cgcgcggcgg agcgccaatc gaaggtcgcc
     7021 gccgggttac aagaactaga cacctggctg caagacgtca ttcgtggcgg aatcgccttg
     7081 gtgcagaaca ggccggccag tttttggaag accccggccg cccgaatggt cgatgcgcag
     7141 gcgcccggtc tggctcggct cgtgcgcgag atgtcgggga ttccggcttc tggcgaaggc
     7201 tggcaggaac gtctgctcaa tcgagtgggt agccttcacc tgatcatcga aggctataag
     7261 cgaattgaaa cactgcctgc cggcacacaa gccgacatcc gtgctcttat cggctgggcg
     7321 caggatcagg acgaagtact cgccggcgaa accgtccgcg atcggtggct ggtgctgggg
     7381 cggcgcgtcg atgaagaaga gcgccttcgg gtccagcgga cgtggttatg gggttgtgag
     7441 tcgcgccgcc cggcgctaat cctgcacttt gcacataaca gccagccgct cgacatgagt
     7501 gttgtgccgg gcaccggctt ggaggccagc atcgtgttct tccccggcgc atatccatta
     7561 cgtgtgctca tcaaagatcg cctcacagca ccgacacctc ttgagtcact gcccggctac
     7621 cgcacgatcg ccgacgcggt tgcggcttac gccaatgcgc tttcgctaaa cccatggctc
     7681 gagcggtttc cgattacgct tgaatcgatc gtcccggtgc accgagacga gcgctggtgg
     7741 atgcgtgatc cgcagggaca tgttcttccg ctggcttctc gcttcatgaa aggctggcat
     7801 ctcgctgcgc tgagcggtgg acaccccatg acgatattcg gcgagtggga tggcgatgac
     7861 cttctgccgt tgagcgcctg gtcagacaat cggttcgtgc aactctagag cgtggccggt
     7921 atgaccgcgt ggcaagacct cattgagact gctttgctcg gcacggaacg gcggcccttt
     7981 aaggtgacgc cgcagggtag cccgctcgac gatatactgt cacggctcga ctccagtaac
     8041 gacgaacgtg ctccgcggag tgttgtggct gcgatgtcga tctatcaacg cgtcggtcgt
     8101 ttgccggtta ccgattctca gccggttccg gcgccgtgtg ccttggatga tgcacctcgc
     8161 tgttcgcccc gcgcggggca gcacctggca ttgttgttag acggccagca tgcccaggtc
     8221 ttgtctgagt ggctcgccgc gctcggtttg acgcaaaagc gcgtgcccga agaaagcttg
     8281 ccggcgctac tcgatctggg gaaggcgcaa cctgacgtgc gcgaagccat ccttccagtg
     8341 ttgggcgagc gtggcctatg gctggcggcg cagagccccg attgggatta cgcgctcggc
     8401 ggcgacgacg cagcggcctg gcagaccggc agcctgccga tacggcttat gctgctgcag
     8461 aaattgcgac gaagcgatac gacacgcgcg cgtgagttga tggcgtcgac gtggccgacc
     8521 gatgcgccgg acgaacgaac ggcttttctg gccgcatgcg cgattaacct gggtacggac
     8581 gacgagcctt tccttgaggc cgcgcttgac gatcggcgca aagaggtgag gcgaatggca
     8641 gccgacctgc tcgcacaatt gccggaatcg cgtctatgtg ggcgcatgct ggatcggctg
     8701 cgcccattgc tgactttgac tcgaggtctg aagcctaaca tcgaagtgac gttgcctgcc
     8761 gagtgcgaca agggcatgct gcgcgacggc gtcgagccga agccgccatc gggtatgggc
     8821 gagaaagcct ggtggctgca gcaaatactc ggagcagtgc cgccatcgat ctggtcgcaa
     8881 acgtggcgca catcgccaaa ggatctggtg caggcggctg ggcgcggtga gtggcggatg
     8941 atgctgttac agggctgggc agtcgccgcg atgcgacaac gggacaccga atggatcgaa
     9001 gcgctgctgg ccgacttcct ggcacataac gagctggccc aggcacagcc ggcttttgaa
     9061 ggtctggatg attctcgcaa agaagcattc gtattgagcc tgctgcactc caacccttcc
     9121 ctgcaaccga tgcaactcgc atatcacttc ctgcttcgct gccgccatcc ctggagtgtc
     9181 gcattgagcc gcgcagtact cgacagcctt cagcgtcata cgtcgaatgg cgacatcggc
     9241 ccacagtttg gctggtcgcc attcctgctg gaggttgcgc gatacatgaa tcctgaactg
     9301 atcgccgaag ccgagtcgcg actgcacgag gccgcagtgt cagggatcga cgcgatcgac
     9361 aatttcctga atctgctgcg ctttcgatac gatatgctga aggagatcca gccatgacca
     9421 acctcttgcg cgagcacgcc gaacagcaat tcgccgaaga gctgcacgag ctggcgaagg
     9481 tcgatacccg cccgcgtccg cctaactgga aactctcgcc gtgggcggtg gcgatgtacg
     9541 tgatgggcgg taagctggag agcggctacg tcgtctcgcc gaaatacatc ggcagccgtc
     9601 gcctgatcga gatcgccatc gccacgctgg cgaccgaccg tgcgttgctg ctgctcggcg
     9661 tgccaggcac ggccaagtca tgggtgtcgg agcatttagc ggtggcgatt tcgggccatt
     9721 cgacgttgat ggtgcagggc acagccgggg cgagcgaaga ggcgatccgc tacggctgga
     9781 attacgctcg actgctggcc gacggcccgt cgatgcaggc actggtgccc agcccgatca
     9841 tgcgtgcgat gcagcaaggc cagttggcgc gcgtcgaaga attgacgcgc atcccgtccg
     9901 atgtgcagga ttccctgatc acggtcttgt cggagaagac actgccaatc cccgaactca
     9961 acaccgaagt gcaggcgatc aaaggcttca acgtcatcgc cacggccaat aaccgtgacc
    10021 ggggcgtgaa cgaactgtcc agcgccctca agcggcgttt caacacagtc gtcctgccgg
    10081 tgccggacag tgtcgagcaa gaagttgaca tcgtccagcg ccgcgtcgag gcgctaggtc
    10141 gcgcgctcga actgccggcc gagacgcccg cgctggagga gatccgccgc atcgtcaccg
    10201 tgtttcgcga gctacgcgat ggcaaatcaa tcgatggcaa gaccaagctc aagtcgccga
    10261 gcgggacgct cagcacggcc gaggcgatct cggtcgtcaa cagcgggctg gcgctggccg
    10321 cgcatttcgg cgatgggcga atgcgcgcca acgatgtcgc ctccggcctg gtcggcgcgg
    10381 tcgtcaaaga tccggtgcag gatcgcgtcg tctggcttga gtacctggag acgatcgtga
    10441 aagagcgaga agggtggaaa gacttgtatc gggcgtgccg aggagtgacg gagtagtgcg
    10501 tacggcgtgt tccgtttctc ggccacgcaa tacgcaacac ggaaagagaa gcctctgtgt
    10561 atcgatcaac gacggtgcat atcttcggca ttcgccatca cgggccgggt tccgcgcgaa
    10621 gcctgcgccg tgcgctcgaa gatctgaagc ccgacgcgat cctcctcgaa ggcccgccgg
    10681 atgccgaagc cgtgctgcca ttgctgctcc aacgcggcat gcagccgccc gtggcgttat
    10741 tcatctacgt gccaggccag cctcagcgcg ccgcgttcta ccccttcgcc gtcttttcgc
    10801 ccgagtggca ggcgttgcac tacggcctgt cgcacaacat cgccgtgcgg ttcatggatc
    10861 taccccaggc gcaccaactg gcgctgaagg aagaagcgtc gcagactccc actcctgagc
    10921 ctcagtctcc tgttcgcaat ccccagtccg aaatgagcct cgatccgctg cgctggctgg
    10981 ccgaggccgc tggtcacaac gatggcgagg agtggtggga gcgtatggtc gagcaacgcc
    11041 gcgacggcac cgatctgttc gccgcgattc tcgaagcaat gacggccctg cgcgagtctg
    11101 tgccggagcc gccggatcct attggaccgc aacgtgaagc ctacatgcga cagacgatcc
    11161 gtgcagcgca gcgccaaggc ttcgagcgca tcgcggtcgt gtgcggcgcg tggcatgtgc
    11221 cggctttaac acccgaagct atcgcccagg cgaaagatga caaggccttg ttgtccgggc
    11281 tgcccaaagt gaaagtcgag gcgacatgga cgccgtggac gtacagccgg ctgttgtact
    11341 tcagcggcta tggcgcgggc gttgtctcgc cgggctggta tcaccatcta tggacgacgc
    11401 ctgaccaggt gactgtgcgt tggatggcgc gcgtcgcccg cctgctgcgt gaagccgatc
    11461 tggacgtttc gccagcgcac ctcatcgagg cagtgcgatt ggccgaatca ctcgccgcgc
    11521 tgcggaatcg cgcgctgcca gggctatccg agttgaatga ggcgacgcaa acggtggtgt
    11581 gttttggcaa cgacacaccc atgcggctga tccatgaccg actcatcgtc ggcgagcggt
    11641 tgggcacagt gccgtcggac acgccgatgc cgccgcttca acgtgacttg gcgcatgcgc
    11701 agaagcgtca gcgcctgccg gtcgatgcgg cccagcgcgc actcgatctc gacctgcgca
    11761 agccaaccga tctggatcgt agcctattgc tgcaccgact caacctgctt ggcgttcgct
    11821 ggggcgtggt ccagcgagct tccactggga aaggcacctt ccacgaagtg tggcagttgc
    11881 agtggaagcc cgaactggct gtgaatgtga tcgaggcgag cgtctgggga aacacagtcg
    11941 tcgatgcggc gacaacgtat gcgcgccatc tagctgacca gacggcggaa gcacccgagc
    12001 cactgcctgc gctgacgcac ctggtcgatc aggtgctgct ggccgatctg cccgatgcgc
    12061 ttccgcacgt gatgggccgc ctgcaagctg agtccgccgt cgccagcgac atcgcccacc
    12121 tgatgaacgc cctcccgccg ctggcgaacg tgatgcgtta tcgagatgtg cgccagaccg
    12181 acatggggat ggtcagtcag gtggtggacg gactggtcgc ccgcatgtgt gtcgggttgc
    12241 cgggcgcgtg cgcgtcgctg aacgacgagg ccgccgccgc gatgttcgat cgattgatag
    12301 gtgtagacag cgcgatggca ttgctgcggg acgacgggta tcgcgcaatg tggcaggcag
    12361 tcgtcgcgca cctggccgat caacacggtg ttcacgggct agtcgccggg cgctgctgcc
    12421 ggttgctgct cgatcaaggt aggttcgttg gcagcgatgt cgcgcgtcga atgagcctgg
    12481 ccctctcccc ggcgaatgaa gcggaacagg cggctgcctg gctagaaggc tttttgaagg
    12541 gaagcgggct gctcctgttg cacgatgagg tgctgtggca ggtgctcgac gactgggtga
    12601 aggcactgcc cggcgaaacg ttcacgcctc tgctgccgct actgcgccgc actttctcga
    12661 ctttcacggc cgtcgaacgc aggcggatag gcgagcgcgc cagccgggga tctgtgtcgc
    12721 gactggaagg gggagggcgg agcgaagagg cttcgttcga ttcggcccgc gccgcgaagg
    12781 ttctgccatt gctggcccaa ttgttggggg tgaataccga tgaacactga aactgagcgc
    12841 ctgcgtcgct ggcgactgat cctcggcacg gaagcggccg aagcgatccc tctggcgctg
    12901 agcggcgacg acatcgtcat cgaccgggcg ctcggcgcgt tgtacggcgc cggtggactg
    12961 agtcggggcg atggagaaga tcgacgcggc gggttagagg gctcggcgcc tcgcgttgca
    13021 cgctggctgg gcgacatccg cacctacttt ccctcatcgg tcgtgcgagt aatgcagcaa
    13081 gacgcgctcg aacgccttaa cctgcgccag atgttgctcc agcccgagat gatggaggcc
    13141 gtgcagcccg acgtgcatct ggtcgccacg ctgctgtcgt tgagcagcgt gatgtccaat
    13201 aaggcgaagg acacggctcg cctggtggtg cgccgcgtcg tcgaagaagt ggagcgccgg
    13261 ctggcgaatg cgctgcgaca ggccgtcacc ggcagcctcg accgcgcggc gcgcaaccgc
    13321 cgcccgcgtt acagcgaaat cgactggcac cgaaccatcc gggcgaacct caagcattat
    13381 cagcccgact atcacagcat tatccccgaa acgcgcatgg gctacggccg caaacgtgcc
    13441 gcactgcgtg acatcatcct gtgcatcgac gaaagcggat cgatggcgac atcggtcgta
    13501 tacgctggca tcttcggcgc agtgcttgca tcgctgaaag cgatccagac gcggctggtc
    13561 gcgttcgata catccgtcgt cgacttgagc caggaact
//
LOCUS       DBNHXB010000474         4219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_994914, whole genome shotgun sequence.
ACCESSION   DBNHXB010000474 DBNHXB010000000
VERSION     DBNHXB010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_994914"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..279)
                     /locus_tag="AC162G_18340"
     CDS             complement(37..279)
                     /locus_tag="AC162G_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HIR5077820.1"
                     /translation="MVNLFDAASGARIGRITEAQLQALVSWMEEESTEDRDYYVSTDT
                     LDMMEQSGIDPTLVAVLRQALGDRDDMDIRYEVEQP"
     gene            complement(346..1641)
                     /locus_tag="AC162G_18345"
     CDS             complement(346..1641)
                     /locus_tag="AC162G_18345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5077821.1"
                     /translation="MSDKSTSPLRPPRIKQPELGRWLESRIGAVCDMWIARVHAYPPT
                     SSGRPLKPPSDAQLRRIFTALCDSLKSGRFTAFDDELAQLTRDGFQMSYRLSDLLYMI
                     LALQDQTWQAITAEFLPERAVVYMQGLSNVLSTALTRIARVFHDEIQAGVAEELERAQ
                     WRLAQLDRAKSNFISIAAHELRTPITLIQGYSDILIQDFIDPADARATAAANGLMTGA
                     RRLLQIVNDMIAVSMIDNESLALNFQPVSLAHVFQIVVGDLRNEIAERSIDVHVAPLP
                     PEVAGRTFYADSQRLYDAFMHLVSNGIKYTPDGGHVRIETRLLSPGRGEEPVVQITVA
                     DDGIGIAPENRERIFEKFHGTTDALRHSSGRTKFKGGGPGLGLAVARGVVEAHGGKIW
                     VESPGYDEVKRPGTTVYVLLPMRAEPPRPRQRLKLDPEG"
     gene            complement(1638..2609)
                     /locus_tag="AC162G_18350"
     CDS             complement(1638..2609)
                     /locus_tag="AC162G_18350"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR5077822.1"
                     /translation="MRNRSTILLFALLTCIILSACGGQGAPVTSTSQIPPTDPVSPPS
                     PLATPLESPIPTLVALEPFDGSKAMEFALDQMSFGPRPTGSEAGRLTGDYIARRLTEF
                     GWLVEAQPFEYLGTSARNIIAARGEGPLILLGAHYDTRRRADEEPTDPTQPVPGANDG
                     ASGVAVLLELARVLNVEATGHRIQLAFFDAEDNGRLDGWDWIAGSRYMADHLEENPSM
                     MVLVDMIGDADQQIYWESNSDPALRERLWAIAAELGYDDHFVPQVKHTILDDHVPFLE
                     RGIPAVDIIDFDYPYWHTAEDTIDKISAASLERVGRTLQVWLERGAP"
     gene            complement(2672..4018)
                     /gene="trmFO"
                     /locus_tag="AC162G_18355"
     CDS             complement(2672..4018)
                     /gene="trmFO"
                     /locus_tag="AC162G_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIR5077823.1"
                     /translation="MNAHSLVVVGGGLAGVEAAWQAAERGIDVALFEMRPVRNTPAHV
                     TDRLAELICSNSLGSDKPASAPGLLKAELRRLGSLVMSCADATAVPAGGALGVDREQF
                     AALVTQRIENHPRIHVWREEVTRVPDEPAIIASGPLTSDALAADMARLSGEEYLYFYD
                     AVAPIVAADSIDMSIAFRASRYGRHSEDASDGDYINCPMTREGYDAFVDALVSAERIE
                     LRSFEQEDPKFFEGCLPIEVMAQRGREALAYGPLRPVGLHDPHTGARPYAVVQLRQDN
                     LAGTLYNMVGFQTNLKWPEQTRVLRMIPGLARAEVMRYGMMHRNTFVNSPAMLEPTLQ
                     YRARPDLFFAGQIVGVEGYVGNAATGWVAGANAARLLKGEGLLVLPRETMIGALCHYI
                     THADPKDFQPMKANFGILPDLDPPVRSKRERYEQYVRRATGVLEAFQHAGAAPGFA"
BASE COUNT          733 a         1301 c         1376 g          809 t
ORIGIN      
        1 atcagtgagc agtcagcagt aattggtaat tggttatcag ggctgctcga cttcgtagcg
       61 gatgtccatg tcgtcgcggt cgccgagggc ttgcctgagc acggcgacaa gcgtcgggtc
      121 gatcccgctc tgttccatca tgtcgagcgt gtcggtgctg acatagtagt cgcgatcctc
      181 cgtcgattcc tcttccatcc agctcacgag cgcctggagc tgcgcctcgg tgatgcgtcc
      241 aatcctggcg ccggaagctg cgtcgaataa gtttaccatg ttcatcctcc gtcagtattc
      301 gccgcgtgtt tctgccgaag ttcaactgcg gcaggagcgg gttcatcatc cctccgggtc
      361 taacttcagg cgctggcgcg gtctgggcgg ctcggcgcgc atcggcagca gaacgtatac
      421 cgtcgtgccg ggtcgcttga cttcgtcgta gccggggctt tccacccata tcttgccgcc
      481 gtgcgcctcg accacgcctc tggcgaccgc caggccgagg cccgggccgc cgcccttgaa
      541 cttggtgcgg ccgctggaat ggcgcagcgc atcggtcgtg ccgtggaact tctcaaaaat
      601 gcgctcgcga ttttccggcg cgatgccgat gccgtcgtcg gcgacggtga tctggacgac
      661 tggctcctcg cctcggccag ggctgagaag ccgcgtttcg atgcgcacgt gcccgccgtc
      721 gggcgtgtat ttgatgccgt tgctaacgag gtgcatgaac gcgtcgtaca gccgctgcga
      781 gtctgcgtag aacgtccggc ccgcgacttc gggcgggagc ggcgctacgt gcacgtcgat
      841 gctgcgctcg gctatctcgt tcctgagatc gccgacgacg atctggaaaa cgtgggcaag
      901 cgacacgggc tggaagttga gcgccagcga ttcattgtcg atcatagaga cggcgatcat
      961 gtcgttcacg atctgaagca ggcgcctggc gccggtcatc aggccgttgg cggcggcggt
     1021 ggcccgcgcg tccgccggat cgatgaaatc ctggatcaga atgtcggaat atccctggat
     1081 gagcgtgatc ggcgtgcgca gctcgtgagc ggcgatgctg atgaaattgc tcttcgcccg
     1141 atcgagctgc gccagccgcc actgcgccct ttccagctct tccgcgactc cggcctggat
     1201 ctcgtcgtgg aagacgcgcg cgatgcgggt caaggccgtg ctcaggacat tgctgagtcc
     1261 ctgcatgtag accactgccc gttccggcag gaactcggcc gtgatggctt gccaggtctg
     1321 atcctggagg gcgaggatca tgtacagcag gtcgctcagg cgatagctca tttggaagcc
     1381 gtcgcgcgtc agttgagcca gctcgtcgtc gaatgctgtg aaacgccccg atttgaggct
     1441 atcgcacagg gccgtaaata tccggcgcag ttgcgcatca gacggcggtt tgagcggtcg
     1501 ccctgacgaa gtgggcggat aggcgtgcac gcgcgcgatc cacatgtcgc agactgcgcc
     1561 gatgcgcgac tcgagccagc ggccaagctc aggctgcttg atgcgagggg gacgcagtgg
     1621 actggtagac ttgtcgctca cggcgctcct cgctccaacc agacctgcag cgtgcgccct
     1681 accctctcca ggctggcggc gctgatcttg tcgatcgtgt cctcagccgt gtgccaatac
     1741 ggatagtcga agtcgatgat gtccacggcc gggatgccgc gctcgaggaa tggcacgtgg
     1801 tcgtcgagta tcgtgtgctt gacctgcggc acgaagtggt catcgtaacc caactcggct
     1861 gcgatcgccc acaggcgctc ccggagggcc gggtcggagt tcgattccca atagatttgc
     1921 tggtcggcgt cgccgatcat gtcgacgagc accatcatcg aagggttttc ttccaggtga
     1981 tcggccatgt atcgcgatcc ggcgatccag tcccagccat ccaggcgccc gttgtcttcg
     2041 gcgtcgaaga acgcaagctg gatacgatgc ccggtcgctt cgacgttcaa aacgcgcgca
     2101 agctcgagga ggacggcgac gccgctcgcg ccatcgttcg cgccgggtac cggttgcgtc
     2161 gggtcggttg gttcctcatc ggcgcgccgg cgcgtgtcgt aatgcgcgcc gaggaggata
     2221 agtggccctt cgccgcgcgc ggcgatgatg ttgcgtgcgg atgtcccaag gtattcgaac
     2281 ggctgcgcct cgactagcca cccgaattcc gtgaggcgcc gggcgatgta gtcgccggtg
     2341 agacgtccgg cttcactgcc cgtcgggcgt ggaccgaagc tcatctggtc gagcgcaaac
     2401 tccatggcct tgctgccatc gaacggctcc aatgcgacca aagtcggtat cggcgactcg
     2461 agcggggttg ccagcggcga gggcggcgag acgggatcag ttggcggaat ctgcgaagtc
     2521 gaggtgacag gtgccccttg cccaccacat gccgaaagga tgatgcatgt cagaagggca
     2581 aacagcagga tggtcgagcg atttcgcata gtgtattgtc ttattcctca gtgttaaata
     2641 ggatataccg gttattgcat agccttgatg actacgcgaa gccgggtgct gctccggcgt
     2701 gctgaaaagc ctcgagcaca cccgtggcgc gtctcacgta ctgctcgtag cgctcacgct
     2761 tgctgcgcac cggcggatcg agatcgggaa ggattccaaa attcgccttc atcggctgaa
     2821 agtccttcgg gtcggcgtgt gtgatgtagt ggcacagtgc gccgatcatc gtctcgcgcg
     2881 gcagcacgag caatccttca cccttcagca gtcgagcggc gttggcgccg gcgacccatc
     2941 cggttgccgc attgcccacg tagccttcga cgccgacgat ctgcccggcg aagaacaaat
     3001 ccggccgggc gcggtattgc agcgtcggct cgagcatagc cggcgagttg acgaaggtgt
     3061 tgcggtgcat catgccgtag cgcatgacct cggcgcgcgc caggccggga atcatccgca
     3121 gcacgcgtgt ctgctcgggc cacttgaggt tggtctggaa gcctaccatg ttgtagagcg
     3181 tgccggccag gttgtcttgc cggagctgca cgacggcata cgggcgcgcg cctgtgtgcg
     3241 gatcgtgcag cccgaccggg cggagcgggc cgtaagccag tgcctcccgc ccgcgctgcg
     3301 ccatcacttc gatcggcagg cagccctcga agaacttcgg atcttcttgc tcgaagctgc
     3361 gcagctcgat ccgttcggcg ctgacaagcg cgtcgacgaa cgcgtcgtac ccttcgcggg
     3421 tcatgggaca gttgatgtaa tcgccgtcgc tggcgtcttc cgagtgcctg ccatagcgcg
     3481 aagcgcggaa agcgatcgac atgtcgatcg aatcggcggc gacgatgggc gcgacggcgt
     3541 cgtagaaata caaatactcc tcgccgctca agcgcgccat gtcggccgcc agtgcgtcgg
     3601 acgtcagcgg gccggaggcg ataatcgccg gttcgtcggg cacgcgggtg acttcctcgc
     3661 gccatacgtg aatacgggga tgattctcga tgcgctgtgt gacgagcgcg gcgaactgct
     3721 cgcgatcgac gccgagcgcg cctcccgccg gcacggcggt cgcgtcggcg caagacataa
     3781 cgagcgagcc gaggcggcgc agctcggctt tcaggagtcc gggcgcagac gcaggcttgt
     3841 cgctgccgag cgagttcgag cagatcagct cggccaggcg atcggtgacg tgcgcgggcg
     3901 tgttgcgcac aggccgcatc tcgaacagag cgacgtcgat gccgcgctcg gcggcttgcc
     3961 aggccgcctc gacacccgcc aggcccccgc cgacgacgac gagcgaatga gcattcatta
     4021 cgcggctatt ctaccactat tcggcgctcc aataacaaaa agccctacca atctaccatt
     4081 attcggcgct ccaataacaa aaggccctac caaccacatg gatggcagga ccttttgcag
     4141 gaggcaacct gcgggagcga cgggattcga acccgcggtc tcggccttga cagggccgca
     4201 tgttaaccgc tacaccacg
//
LOCUS       DBNHXB010000475         4763 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4351225, whole genome shotgun sequence.
ACCESSION   DBNHXB010000475 DBNHXB010000000
VERSION     DBNHXB010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4351225"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="AC162G_18360"
     CDS             complement(<1..1130)
                     /locus_tag="AC162G_18360"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005453445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase family protein"
                     /protein_id="HIR5077824.1"
                     /translation="MTNQTETQTAALTGADRSDTLIRPFRIDIPQADLDDLQARLTMT
                     RFTDEIPGSGFDYGVSVEWVKRMVDYWRAGYDWRAWEAKLNTCPQFTTEIDGQNIHFI
                     HVRSAKEDALPLILTHGWPGTVVEYLDVIEDLSQDFHLVIPSVPGFGFSGPTREKGWN
                     RYRIARAWAELMKRLGYDRYGAVGNDGGSFVSPEVGRINPKHVVGVHVTQIFSFPSGD
                     LAEFEGLTEEEQAAVRHLQWFWENMGAFNQLQSQSPQTLAHAIADSPAGLLGWMGQLL
                     QGLDDDFVLTNITIHWLCRTAGSSIRFYYEDAKAEHPTGPTTVPIGLAGFANDFQSIR
                     RFAARDHKNIVQWHTYDVGGHYAAHQVPKVLAKDVREFFSGLR"
     gene            1226..1750
                     /locus_tag="AC162G_18365"
     CDS             1226..1750
                     /locus_tag="AC162G_18365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR5077825.1"
                     /translation="MSNSILQRQHAVKSGAPDTLDASADIPPRTPAGDAFSAFIVQVF
                     QLNGLLLAAGDALARPAGQTSARWRVLAAVEDAPSTVAQIARAWRLARQSVQRVADVL
                     VREGLAVYEKNPSHRRAKLLRLTPRGRSALRTIQTAQRVWADALGAEIGEADLRQASA
                     VLARASRVVMSRHR"
     gene            complement(1774..2034)
                     /locus_tag="AC162G_18370"
     CDS             complement(1774..2034)
                     /locus_tag="AC162G_18370"
                     /inference="COORDINATES: protein motif:HMM:NF026093.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077826.1"
                     /translation="MDAEFKVVDVLEAEGNFAGAAIYVCVTPEGRQFTVNPDGNLETR
                     REIFRNRRRYLGKRVTVRFQNYSDDRLPRFPTRATVRERVQG"
     gene            complement(2053..2184)
                     /locus_tag="AC162G_18375"
                     /pseudo
     CDS             complement(2053..2184)
                     /locus_tag="AC162G_18375"
                     /inference="COORDINATES: protein motif:HMM:NF013252.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2448..2876
                     /locus_tag="AC162G_18380"
     CDS             2448..2876
                     /locus_tag="AC162G_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR5077827.1"
                     /translation="MSHPLDDYIGYRILQIHRAHRSRAEAALNQLGLHTGQEMLLFHL
                     WNEEGLTQSQLVDQLCVEPPTVTKTLQRLEQAGLVERRQDAEDARVSRVYLTPKGRAL
                     KAQVKKIWENLEALTVQGLSEVEKALMRRLLDQINNNLAR"
     gene            2971..3870
                     /locus_tag="AC162G_18385"
     CDS             2971..3870
                     /locus_tag="AC162G_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015195671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HIR5077828.1"
                     /translation="MVTKSEAIILVSGVTGNQGGATARHLLANGWPVRGLTRDARKPA
                     ALAFAEQGVEIIEGHFDDRASLDRSLEGVYGVFSVQSGFEAGLEAEERWGKAFADAAK
                     AANVQHFVYSSVGGAEKNTGIPHFESKRRIEEHLQALGLPATILRPVFFMENFNFMSR
                     QSVFEQGKLSFALRPAKKLQMIAVDDIGAMAALVFENPEIYIGQAIEIAGDELTMPET
                     AELFSRVTGRPVTFEEQPLEPLKEYSYDLYAMQKWFNEAGYQADIARLRRLHPGMQTL
                     EQWLQSNGWANDQSAGRQHEQLA"
     gene            3830..>4763
                     /locus_tag="AC162G_18390"
     CDS             3830..>4763
                     /locus_tag="AC162G_18390"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5077829.1"
                     /translation="MTSLLGGSMNNWLEAFRQSFSPLRIRNFRIYLGGQAISLVGTWL
                     QMTAQGIVVYELSQGAATPLGIVGMLSTLPILILSPWTGVWADRLDRRRLLIGTQVGA
                     MLLAFSLAVLTQTGLIQLWHVYVLASLLGIFSAIDFPAQQAFIGDLSGMAEVRKAVNL
                     NGITLQASRMLGPALAGYMIGALGAASAFWFNGLSFMVVIISLMIVHAHQVRSTEDSR
                     HSSGFGQALDFIRSQPHLQDLFVFVFLIAFFGLAVMNIVPAIARGQPEATGLLLAAAG
                     LGALVSAVFVVPLTLAVRRAGLVVSNAVLWMGVWF"
BASE COUNT          911 a         1443 c         1501 g          908 t
ORIGIN      
        1 cgaaggccgc tgaagaactc acggacgtcc ttcgccagta ccttgggcac ctggtgcgcg
       61 gcgtagtggc cgccgacgtc gtaggtgtgc cactgcacga tgttcttgtg gtcgcgcgcg
      121 gcgaagcggc ggatcgactg aaagtcgttg gcgaagcccg cgaggccgat cgggacggta
      181 gtcggcccag tcgggtgctc ggccttggcg tcctcgtagt agaagcggat tgaggagccc
      241 gccgtacggc acagccagtg gatcgtgatg ttcgtcagca cgaagtcgtc gtcgagacct
      301 tgcaggagct ggcccatcca gccgagcagc ccggcgggtg agtcggcgat cgcgtgggcg
      361 agcgtctgcg gcgattggga ctggagctgg ttgaaggcgc ccatgttctc ccagaaccac
      421 tgcagatgcc gaaccgcagc ctgctcctcc tccgtcaggc cctcgaactc ggccaggtca
      481 ccggagggga aggagaagat ctgcgtgacg tgcacgccga cgacgtgctt ggggttgatc
      541 ctgccgacct cgggggagac aaacgagcct ccgtcgttgc caaccgcgcc gtagcggtcg
      601 tagccgaggc gcttcatcag ctcggcccac gccctggcga tccggtagcg gttccagccc
      661 ttctccctgg tagggccgga gaagccgaag ccgggcacag acgggatgac caggtgaaag
      721 tcctgggaca ggtcctcgat cacgtcgagg tactccacga ccgtcccggg ccagccgtgg
      781 gtgaggatca gcgggagcgc gtcctccttg gcggaacgga cgtggatgaa gtggatgttc
      841 tgcccgtcga tctcggtggt gaactgcggg cacgtattga gcttggcctc ccacgcgcgc
      901 cagtcgtaac cggcgcgcca gtagtcgacc atccgcttga cccactcgac gctgacgccg
      961 tagtcgaatc cgctgcccgg gatctcgtcg gtgaagcggg tcatcgtcag ccgcgcctgc
     1021 aggtcgtcca gatcggcctg tgggatgtcg atgcggaaag gtcggatcaa ggtgtcggag
     1081 cggtcggcgc cggtcagcgc ggcggtttgc gtctcagtct ggttggtcat gttgggcatc
     1141 gaagtctcct ttcttcatgg ttcgcaggaa tcgcaaccac ggatcgaggc ttgcgcggga
     1201 gcgtgagata tggcagaata ctgtcatgtc gaacagtata ctacaacgac agcatgctgt
     1261 caagtccggc gcgccagata ccctcgatgc gtccgccgac atcccacccc gcacgccagc
     1321 aggcgacgcc ttcagcgcat tcatcgtgca ggtgttccag ctcaacgggc tccttctcgc
     1381 cgccggggac gcgttggcca ggcccgcggg ccagacgagc gcacgctgga gggtcctcgc
     1441 ggcggtcgag gacgcaccct cgacggtggc gcagatcgcg cgcgcttgga ggctcgcccg
     1501 acagagcgtg caacgcgtgg cggacgtgct cgttcgggaa ggattggccg tgtacgagaa
     1561 gaaccccagc caccggcgcg ccaagctctt gcggctcacg ccacgcggcc gctccgcgct
     1621 gcgcacgatc cagaccgcgc agcgggtgtg ggctgatgcg ctgggcgcgg agatcgggga
     1681 ggccgacctc cgccaggcaa gcgctgtcct cgcccgtgcg tcacgagttg ttatgagccg
     1741 gcaccggtaa ggataccaag tagaggaagc ccctcacccc tgcacccgct cgcgcaccgt
     1801 cgcccgcgtc gggaagcgag gcaggcggtc gtcggaatag ttctggaagc gcactgtcac
     1861 ccgcttgcca aggtatcgcc gccggttgcg gaagatttct cggcgagtct ccaggttgcc
     1921 atcggggttg acggtgaact gccgcccctc gggggtcacg cacacataga tggccgcccc
     1981 ggcgaagtta ccctccgcct ccaacacatc cacgaccttg aattcggcgt ccatgaaggc
     2041 cttgtacttt tgcagtccga ccacccggtc gccgggcttg tacactgcgc tcgggtcgcg
     2101 caggatcagc ccttcgtacc cctggccggc gaagtagtca tgccagcgcg gcaactcgtc
     2161 ggcggaatgg atggggacgg tctcgacttg tacaaaatat cgcgcggttt atcaaatacc
     2221 cgccgactac ataggggcaa ctaacatcat atgtattcta cattcatgtg tctggaggcc
     2281 attcgagatg cttcggcgtt gcgcgccaca gcatgacagg cggctggcct aagtgacaaa
     2341 gtagtactaa gagacagggt cgatactgtc caatgggtca taccagaagg gttgacaact
     2401 aacttcttcg gtgctactat ttagccagct aaataaacga gcgggtgatg agccaccctc
     2461 tagacgacta tatcggttac cgcatccttc aaattcacag agcacatcgt agccgcgccg
     2521 aagccgctct caatcagttg gggctgcata ccgggcaaga aatgctcttg ttccatctct
     2581 ggaacgaaga gggtctcaca caatcgcagt tggtcgatca actgtgcgtc gagccgccca
     2641 ctgtcaccaa gacgctccag cgcctggaac aagccgggct ggtggaacgc agacaagacg
     2701 ccgaggacgc acgtgtttcc cgtgtgtacc tcaccccgaa ggggcgcgca ttgaaagcgc
     2761 aggtgaaaaa aatctgggag aacctcgagg cacttactgt gcaaggctta tctgaggtcg
     2821 aaaaagcgct gatgcgccgc ctgcttgacc agattaacaa caatctcgcc agataggtcg
     2881 tttactgttc ctcgctttgc gaggcaaatt ttggccataa tactttgccg gctaaccaat
     2941 caacaatctg atcatctcaa ggagagacac atggttacca aaagcgaagc catcatcctc
     3001 gtcagcggtg tgacgggtaa tcaaggcggc gcgacggcgc ggcacctgtt ggcgaacggc
     3061 tggccggtgc gcggattgac gcgcgatgcc cgcaagcccg ccgccctagc ctttgccgag
     3121 caaggggtcg aaatcatcga aggccatttc gacgaccgcg cctcactgga cagatcatta
     3181 gaaggcgtct acggcgtgtt cagtgtgcaa agcggctttg aggcaggcct tgaagccgaa
     3241 gagcgctggg gcaaggcgtt tgccgacgcg gcaaaggcgg cgaacgtaca gcatttcgtc
     3301 tattcctcgg tcggcggcgc agaaaagaac actggcatcc cgcatttcga gagcaagcgg
     3361 cgtatcgagg aacatcttca ggcgctcggc ctgcccgcga ccatcctccg tccagtgttc
     3421 ttcatggaaa acttcaactt catgtcccgc cagtccgtct tcgagcaggg aaaactcagc
     3481 ttcgccctgc gcccggccaa gaagctgcag atgatcgccg tggatgacat cggcgcgatg
     3541 gcggcactgg tcttcgagaa tcccgaaata tacatcggcc aggccataga gatcgccggt
     3601 gatgaattga ccatgccgga gacagccgaa ctgttctcgc gcgtcacggg tcgtccggtc
     3661 acattcgagg agcagccgct tgaaccgctt aaggagtaca gctacgacct gtacgcgatg
     3721 cagaagtggt tcaacgaggc gggttatcag gcagacattg cccgtctgcg ccgtttgcat
     3781 ccgggtatgc agacgttaga gcaatggcta cagagcaacg gttgggcaaa tgaccagtct
     3841 gctgggcggc agcatgaaca actggcttga ggcattccgg cagagtttct cgcccttgcg
     3901 catccgcaac ttccgcatct atttgggcgg gcaggctatc tcgctcgtcg gcacctggtt
     3961 gcagatgacg gcacagggca tcgtggtata cgaattgagc cagggcgcag ccacgccgct
     4021 gggcatcgtg ggcatgttga gcacgttacc cattctgatc ctcagcccgt ggacgggggt
     4081 gtgggcggat cggttggacc gccgccggct tttgatcggg acgcaggtgg gcgcaatgct
     4141 gctggccttc agcctggcag tgctcactca aaccggcctg atccaactct ggcacgtcta
     4201 cgttctggcg agcctgctcg gcatcttcag cgccattgac ttcccggcac agcaagcctt
     4261 catcggcgac ctttcgggca tggccgaagt gcgcaaagcc gtcaacctga acggcatcac
     4321 gttacaagcg agccgcatgc tcggcccggc actggccggt tacatgatcg gggcgctcgg
     4381 tgcagcctcc gcattctggt tcaatggcct aagcttcatg gtcgtcatta tcagcttgat
     4441 gatcgtgcac gcccatcagg tgcgctcgac tgaggacagc cggcacagca gcggcttcgg
     4501 ccaagcgctt gatttcatcc gcagccagcc acacctccaa gacctgtttg tctttgtgtt
     4561 cctcatcgcc ttcttcggcc tggccgttat gaacatcgtt ccggccatcg ccaggggcca
     4621 acccgaggcc accggtctgt tactggcggc cgcgggactg ggtgcgctgg tgagcgccgt
     4681 gttcgtcgtc cctctcaccc tggctgtgcg gcgcgccggc ctggtggtga gcaatgcggt
     4741 gctgtggatg ggagtgtggt ttg
//
LOCUS       DBNHXB010000476         8691 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4685423, whole genome shotgun sequence.
ACCESSION   DBNHXB010000476 DBNHXB010000000
VERSION     DBNHXB010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8691)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4685423"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="AC162G_18395"
     CDS             <1..250
                     /locus_tag="AC162G_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077830.1"
                     /translation="AIAQARAKAVAAAPAVRQLVDFRVGDALYPAQFPGPFGAVVDNT
                     YWAGSGPDTGGAYYPVVEFKAQGRKARALHGRHRQSAA"
     gene            213..452
                     /locus_tag="AC162G_18400"
     CDS             213..452
                     /locus_tag="AC162G_18400"
                     /inference="COORDINATES: protein motif:HMM:NF023580.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HIR5077831.1"
                     /translation="MRFTDGIGNLPPEYEVGAQVDVLYNPKDVDEARISSWVRLWLAP
                     TLLTFVGKLPILIGIGVAWAVRRRFKSHSFLSRAA"
     gene            486..1358
                     /locus_tag="AC162G_18405"
     CDS             486..1358
                     /locus_tag="AC162G_18405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR5077832.1"
                     /translation="MNITTASVEPGAAQRPARSAQRGMVIYLVVAFGFTWAILGLGIL
                     AARGVITLPLSTTGLVTLATLGPMIAALSATAFEAGRAGVRTLLAQVVRWRVRPIWYG
                     LALGVPAMIMLSAFVLWRVLGGPALPAPPIQSWLSLPVLVMALVIPALFEEIGWRGYA
                     LPRLQSRTNALAASLMLGLIHACWHVPLWFIPGLGFDSLPFPLYALLVIGLSILFTWL
                     YNSTGGSLLIVGLFHAAINAYPAPWGAALQTLPESARGTNIQIAVAVTAAVFAICVVL
                     LTHPRTLTRQRETG"
     gene            1497..1958
                     /locus_tag="AC162G_18410"
     CDS             1497..1958
                     /locus_tag="AC162G_18410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077833.1"
                     /translation="MPVAAPLLTTKLYIPRVRSKLVPRLRLTARLNEATEGSFTLISA
                     PPGFGKTTLLAEWIPSGERCVIWDSLDAGDNEPARFWSYVIAALQMLNVRLGENVQAL
                     LQSPQMPSTEFLLKLLLNDIAAFPQAFALVLDDYHVIASGVSCHTRTSSAR"
     gene            1928..4417
                     /locus_tag="AC162G_18415"
     CDS             1928..4417
                     /locus_tag="AC162G_18415"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5077834.1"
                     /translation="MSYTNVVSPLTSFIGRRQEIVAVKQLLAQARLLTLTGAGGCGKT
                     RLAVQTALELAEADVFVDGVWWIELASLSDATFVPQSVARALGVSGAPDQPLIETLAS
                     FLKTRHLLLVIDNCEHLLKACAQLVETMLLAAPAVRILATSREPLAVAGETIWLTPSL
                     STPDPEHLSRPPADLLSDLAEYDAIHLFVERAASVRPTFALTRHNAAAVTRTCHRLDG
                     IPLAIELAAARVNVLTVDQIAARLDNCMTLLASGSRTALLPRHRTLRATIDWSHDLLP
                     DEERTLFRRLSALAGGFTLEAAEAICAEGGLAHEEILDVLSHLVNKSLVVAETEERSE
                     ARYRLLEPIRQYAWEQLVESGEEALVRKRHLNWFLALAEQAEAEWRGPSQKALLDQLE
                     TEHDNLRAALEWSNTDEDKTEARLRLAGALSWFWNVRGYLSEGRRWLEDALTRTEESA
                     GRTLARAKALYGAGVLAFTQGDYVVARPRLEESVTIWQERGDQRGLAHSLVHVGLVAL
                     RQGDYASAHSRLEESRALFEEVEDQWGVALALNHLGNLASRQRDYAAAHSLLTGALAL
                     CRAVGDKRLTATSLNNLGEVARCEDDDERASVLYEEALALRRELNDKSGIATTLHNLG
                     HVLQHQRDHKKAAALLEESLAVYQEVGDKRGLGLCLAGLAGIAMQTSLGSSSRYDEDL
                     RRAVQLFGAADGLLNGISASLAPADRAEYDRNVATLRFQLGEAAYAAAWAEGRAMTLE
                     QAIAEARMPAPVDLPPPSTLRHQPLLEPLTDRELEALRLVAEGLSDRQIAERMTVVVG
                     TVKRHLNNVYGKLGVHRRTEAVARARELGLL"
     gene            4567..4833
                     /locus_tag="AC162G_18420"
     CDS             4567..4833
                     /locus_tag="AC162G_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015234719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HIR5077835.1"
                     /translation="MAIYMADRSLPGITLEQIAAAQKAALETSERFTAEGKPVRYIRS
                     VFVPEEAHLMCLFEAADARLVRDVNEAAGLPFTRIIEALDLSPA"
     gene            5063..5521
                     /locus_tag="AC162G_18425"
     CDS             5063..5521
                     /locus_tag="AC162G_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin family protein"
                     /protein_id="HIR5077836.1"
                     /translation="MNDTQKLLVRASFEKVLPIANAAAALFYRRLFELDPSLKPLFKG
                     DMREQGRKLMSAIQLVVAGLDRLDKIVPAVQHLGRRHAGYGVKPEHYRTVGAALLWTL
                     EQGLGEAFTPETKDAWIAAYTLLSDTMIAAAEDEVVSIAAPATVAMAPTA"
     gene            5838..>8691
                     /locus_tag="AC162G_18430"
     CDS             5838..>8691
                     /locus_tag="AC162G_18430"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7933 domain-containing protein"
                     /protein_id="HIR5077837.1"
                     /translation="MTSDNMPEPLIGLSLDRYQILALLGQDALGFSYKAHDVEFQREV
                     VVKVIDPRIAEQPDFEAQFSLAARTAIGLRHPGLINVFDFGQARSLYYIVTEYIPGGN
                     LGTALQDLRAAGKWIALSEAVQLVRHVCLALDYARRNGVPHRSVEPSHILLKLQPGDD
                     LGYQPVLDDLGLAELRPGEARPLEDFSPSELTYLSPEQARGEAPDARSSVYSAGVLLY
                     QLAVARPPFRIRTAVEAARYHAEETPPAPRSIRADLPESIERIIVSALEKDPARRFAT
                     AGEMAQALAQTLTAAVEVDARPETQAWAVSLLTQLQPAGEELPESPASATIEPELAFK
                     APPVKGGFTRADGSAIDPGQIRFSAGERVGLFVEPALLTVVPGNSVTLSLIVFNRSAT
                     DDRFTVSLTGAPANWISEPLPEVELAPGAQAEVKQSIRLPRSPQSRAGRRALTVRVVS
                     QDEPDEVVEERVTLNIVVYSEFSSDLRPQRVRVGETARVVVHNHGNTPENFRLACFDP
                     AGKLAFEPAELSLTVPEGQGITAEFIAGLRRRRLVGGTRADGFLARIVAQSGQAQTHT
                     GEVISAGLIPAWIVSLVLLIMFAGLVVGAAALLLTPPTPSTAAAPVIQKAFSNVNGLN
                     STVTFTLTNPNATLPLTGVAFTDTFPTMPGAMVVANPPNAIVSTGCGAPSFVADAGSG
                     SITFVNGTITAGGTCTLAVDATVPAAGTYDNTTGPVTSIEGGTGDSASASLMVSALAP
                     PSIAKSFSLTSIPAGAVVKLTFTIVNPNPGITLTGVTFTDVFPATPGAMVVANPPNPS
                     ISAGCGLPAFTPEAGSGSITFSNGTIAGGSACVVTIDVTAPVGGTYTNVSGAVVSANG
                     GTGNTATAVLTVGAAAPPGIAKSFAPSSIVAGMKSTLTFTVTNPNAGVTLSGVAFGDT
                     FPAGMVVANPPNPSVSAGCGLPAFTPAAGSGSVAFSN"
BASE COUNT         1546 a         2677 c         2830 g         1638 t
ORIGIN      
        1 cgcaatcgcg caagcccgcg ccaaggcggt tgccgctgcg ccggctgtcc gacagttggt
       61 agacttccgc gtcggcgacg cactctatcc cgcccaattc ccgggaccgt tcggggcggt
      121 ggtcgacaac acctattggg ccggcagcgg cccggatacg ggcggagcat attaccccgt
      181 cgtcgagttc aaagcccaag gacggaaagc tcgtgcgctt cacggacggc atcggcaatc
      241 tgccgcctga atatgaagtg ggtgctcagg tggacgtgct atacaatcca aaggatgtcg
      301 acgaggcgcg aatcagctcg tgggtgcgcc tgtggctcgc gcctacgctc ctgactttcg
      361 tcggtaagct gcccatcctg atcggcatcg gcgtggcgtg ggccgtgcga cgccgcttca
      421 aatcacactc gtttctatca cgagctgcct gaactcggca ctggacgaat gataaggaga
      481 ttaccatgaa tatcaccaca gcgtccgtcg aaccaggtgc agcacagcga cctgcacgat
      541 cggcacagcg cgggatggtc atttacctgg tcgttgcctt cggttttacc tgggctattc
      601 taggattggg cattctcgcc gcgcgcggcg tcataacgct gccgctttcc actaccggcc
      661 tggtgacgct tgcaacgctg gggccgatga ttgcggcgct gagtgccacg gcatttgaag
      721 ccgggcgcgc gggcgtgcgc acgctgttgg cgcaagtcgt gcgttggcgc gttcgtccca
      781 tctggtatgg cctggcgctg ggtgtgccgg cgatgatcat gttgagcgct tttgtgttgt
      841 ggcgcgtatt gggtggaccg gcgctgcctg cgccgccgat ccaaagctgg ctatcgctcc
      901 ccgtcctcgt catggcgtta gtcatccccg cgctgtttga ggaaatcggg tggcgcggct
      961 atgcgctgcc gcgtctacaa tcccgcacca acgcgctggc cgcgagcctc atgctgggac
     1021 tcattcatgc ctgctggcat gtgccattat ggtttatccc cggcctgggc tttgacagct
     1081 tgcctttccc cttgtacgcc ttgctggtga ttgggttgtc gatcttgttc acgtggctgt
     1141 acaatagcac gggtggtagt ctgctcatcg tcggattgtt tcacgccgcc atcaatgcat
     1201 atcctgcacc gtggggcgcc gcactgcaaa ctctgcctga aagcgcccgg ggcacgaata
     1261 tccaaatcgc tgtcgccgtg acagccgccg tctttgccat ctgcgtggtg ctgctgaccc
     1321 atccacgcac gctcacacgc cagcgtgaaa cgggctaatt aagatcaact ttcgtagtcc
     1381 atatggcggt gctgctggcg ggcgccatct agcagcaggc agaataggct gagacggttg
     1441 gcacatcaat cctccctcgg ctataatcat gatagtcctg ccgagggaga aagctggtgc
     1501 ctgtcgccgc gccgcttctc acgaccaaac tgtatattcc ccgcgtacga tcgaagctcg
     1561 taccgcgcct gcgtctgacc gcgcgactga acgaagcgac ggaaggttcg ttcacgctga
     1621 tctccgctcc ccccggcttc ggcaagacga cgctgctggc cgaatggatt ccatctggcg
     1681 agcggtgcgt catctgggat tcgctggatg ctggcgacaa cgaaccggcc cgcttctggt
     1741 cgtatgtcat cgccgcgcta cagatgttga atgtccgcct cggtgaaaac gtgcaggcgc
     1801 tgcttcagtc gccccagatg ccgtccaccg aattccttct gaagctgctg ctgaacgaca
     1861 tcgccgcgtt cccgcaggcc ttcgcgctcg tcctggacga ttaccatgtc atcgcttccg
     1921 gggtgtcatg tcatacacga acgtcgtcag cccgttgacc agtttcatcg ggcgcaggca
     1981 agagatcgtc gccgtgaagc aattgcttgc gcaggcgcga ttgctgacgc tgacgggtgc
     2041 cggcggctgc ggcaagacgc gtctcgccgt gcaaaccgca ctcgagttgg cggaggcgga
     2101 tgtgttcgtg gatggcgtct ggtggatcga attggcttcg ttgagcgatg caacgttcgt
     2161 gccgcagtcg gtggcgagag cactgggtgt ctccggcgcg ccggatcaac cgctgatcga
     2221 aacactcgcg agcttcctta aaaccaggca tttgctcctc gttatcgaca actgtgagca
     2281 tctcctcaaa gcgtgcgcgc agctcgtgga gacgatgctc cttgctgcgc cggctgtgcg
     2341 gattctggcg acgagccgcg agcctctcgc tgtcgccggc gagacgattt ggctgacgcc
     2401 ttccttgtcc acgcctgatc ccgagcatct gtcacgcccg ccggcagacc tcttgtccga
     2461 tctggcggag tacgatgcga ttcacctctt tgtcgaacga gccgcttccg tgcgtccgac
     2521 cttcgcgctg acccgccaca acgcggcggc agtgacccga acatgccatc gcttggacgg
     2581 tattcctctc gcgatcgaac tggccgcggc gcgggtcaac gtattgacgg tcgatcagat
     2641 tgccgcgcgg ctggacaact gtatgacttt gctcgcctcc ggcagccgaa ccgcacttct
     2701 gccccggcat cggactctgc gggcgacaat tgactggagc cacgatctat tgcccgatga
     2761 ggagcggacc ttgttccgtc gtttgtcggc gcttgcgggc gggtttacat tggaagccgc
     2821 ggaagcgatt tgcgccgagg gcggacttgc ccacgaggaa atcctggatg tgctatcgca
     2881 cctggtgaac aaatccctgg tggtggcgga gacagaggag cgaagcgagg cgcgctatcg
     2941 cttgctggag ccgatccggc aatatgcgtg ggagcaattg gtcgaatcgg gcgaagaggc
     3001 gctggtgcgc aagcgtcatc tcaactggtt tctcgcgctg gcggagcagg cggaggccga
     3061 gtggcgcggg ccaagtcaga aggcgttgct cgatcaactg gagacggaac acgacaacct
     3121 gcgggcggcc ctggagtgga gcaacaccga cgaggataaa acagaagcga gattgcgatt
     3181 ggccggggcc ttgtcgtggt tctggaacgt gcgtggctat ttgagcgagg gacgccgatg
     3241 gctggaggac gccctgacgc ggacagagga atcggctggg cgcacgctcg cgcgagcgaa
     3301 ggcgctgtac ggcgcgggcg tgctcgcctt cacccagggc gactatgtgg tcgcgcgccc
     3361 tcgcctcgag gagagcgtga ccatttggca agagcgagga gatcagcgag gccttgccca
     3421 ctcgctcgtc catgtgggac tagtcgcact acgtcaaggc gactacgcct cggcgcactc
     3481 ccggcttgag gaaagccggg cgctcttcga ggaagtcgag gatcagtggg gcgtcgccct
     3541 ggcactcaat catttaggga acttggcctc tcgacagcgc gactacgccg cggcgcactc
     3601 ccttcttacg ggagccttag ccctgtgtcg agccgtaggg gacaagcggc tcaccgcgac
     3661 atcgctgaac aacttggggg aagtcgcacg atgcgaggat gacgatgagc gggcgtcggt
     3721 gctctacgag gaggctctgg ccttacggcg agaactgaat gacaagtcgg gcattgccac
     3781 cacgctccat aatttgggac atgtattgca gcaccaacgt gatcataaaa aggccgccgc
     3841 gcttcttgag gagagcttgg ccgtgtacca ggaagtggga gataagcggg gcctaggctt
     3901 atgcctggca ggattggccg ggatcgccat gcaaacgtcg ttagggtctt cttcgcgata
     3961 tgacgaagac ctgcggcgcg ccgtgcagtt gtttggggca gcggatggcc tcctgaacgg
     4021 tatcagtgct tccttagcgc ccgccgaccg cgctgaatac gaccgaaatg tggctacctt
     4081 gcgcttccag ctcggcgaag ccgcgtacgc cgccgcctgg gcggagggac gggcgatgac
     4141 gctggagcag gcgatcgcgg aggcccggat gccggcgccc gtcgaccttc cgccgccgtc
     4201 gaccctccgt catcagcctt tgcttgagcc gctgaccgac cgcgaactgg aagcgctgcg
     4261 cctggtcgcg gagggcctgt ccgaccggca gattgcggaa cggatgaccg tcgtcgtggg
     4321 cacggtgaag cggcatctga acaacgtcta cggcaaactc ggtgtgcacc gccgcactga
     4381 agcggtggcg cgcgcccgcg aattgggcct cttgtagcgc cccctcacat cccccaaatc
     4441 aaccgtccaa cacatccttc gatacatgac gaccggcctg gttgaatggc atactgtggc
     4501 tgttgctgga tagtggtcag caagagcacg caactccgca cctgatcgag gaaggaggtg
     4561 aaaaccatgg ccatctatat ggctgatcgc agtctgcccg ggatcacgct ggagcagatc
     4621 gccgccgcgc agaaagcggc gctcgagacg agcgagcggt tcacggcgga gggcaagccg
     4681 gtgcgttaca ttcgcagcgt gttcgtgccg gaagaggcgc acctcatgtg cctgttcgaa
     4741 gcagcggatg ccaggctggt gcgcgatgtg aacgaggccg ccggcctgcc attcacccgg
     4801 atcatcgaag cgctcgacct atcaccggcg taaccgcggt ggcgcgtggg gcagcgggcg
     4861 tcccaaaagc gcccgctgct tcaccggctc acagccgact ccacggcgtc aggccgtgtg
     4921 gctagttgat agtcttgttc ccgcagcgcc cctcctcgcc ctttcccaaa agatcaaccc
     4981 ctcaatgtgt ctttcgatat gtgacaacat gcctggttga cgcctatact cggcgcataa
     5041 cgagatcaga agggagacaa tgatgaacga cacacaaaaa ttgctggtgc gcgcctcgtt
     5101 cgagaaggtg ctgcccatcg ccaatgcggc cgccgcgctg ttctatcgcc ggctgttcga
     5161 gctcgacccc tcgctcaagc ccctgttcaa gggcgacatg cgggagcagg ggcgcaaact
     5221 gatgagcgcg atccagttgg tcgtcgccgg cctggatcgg ctggacaaga tcgtcccggc
     5281 cgtgcagcac ctgggtcggc ggcacgcagg ctacggcgtc aagccggagc actaccgcac
     5341 ggtcggggcc gcgctgctgt ggacgctgga gcagggcctg ggggaagcct tcaccccgga
     5401 gacgaaggac gcctggatcg cagcttatac gctgttgtcc gacacgatga tcgctgccgc
     5461 tgaggacgag gtcgtgtcga tagccgcgcc ggctactgta gcgatggcgc ccaccgcgta
     5521 agcgtgtatg aagaggcttg actataacca caaattcatt gaagatgtgg catttcgagc
     5581 cacggccagc gggttggtct ccgagcaggc cgcttcaacc ggcctagaga acgccgcgtt
     5641 tgagcaagcg gacggtgaaa agtagctttc cttctcgacg ccaggcttac atctccggtc
     5701 gaccttgacc atgacgcggg agccgtgctc cggacggctc ccgcggctgc gttgtgaaca
     5761 tcgacagcag aactgtgcca ggatttggga tcgtggtcgt catgcgttaa aataggcgaa
     5821 agcggataac accagacatg acgagcgaca acatgccgga gcctctcatc ggcctctcct
     5881 tggaccggta tcagatcctg gctctgctgg gccaggatgc actcgggttc tcctacaagg
     5941 cccacgacgt cgagtttcag cgcgaggtcg tggtcaaggt catcgatccg cgcattgcgg
     6001 agcagccgga cttcgaggcg caattctcgc tggccgcccg cacggcaatc ggcctgcgtc
     6061 acccagggct tattaacgtt ttcgacttcg gccaggctcg atcgctgtac tacatcgtga
     6121 cggaatacat cccaggtggt aacctgggca cggcgctcca ggacctgcgc gcagccggta
     6181 agtggatagc gctatcggag gctgtccagc tcgtccgtca tgtctgcctc gctctcgatt
     6241 acgcgcgccg gaacggcgtc ccgcatcgca gcgtcgagcc gagccacatc ctgttgaaac
     6301 tccagccggg tgacgatctg ggctaccaac cggtgcttga cgacctgggg ctggcggagc
     6361 tgcgcccagg cgaggcccgg ccgcttgagg acttctctcc gagtgagctg acatatctct
     6421 cgccggagca ggcgaggggc gaggcgcctg atgcgcgcag cagcgtgtac tccgccggag
     6481 tccttctcta ccaactggca gtcgcccggc cgcctttccg gatcaggact gctgtcgaag
     6541 ccgctcgtta tcacgcagaa gagactccgc ccgcgccgcg atcgatccgc gcagacttgc
     6601 cggaatctat cgagcggatc atcgtcagcg cgctcgaaaa ggatccggcc cgtcgcttcg
     6661 ccaccgccgg ggagatggct caggcgctgg cccagaccct gacggccgca gtggaggtcg
     6721 acgccagacc tgagacacag gcgtgggccg tcagcctgct gacacagttg cagccggcgg
     6781 gggaggaatt gccggaatct ccggcgtccg cgaccatcga gcctgagctc gctttcaaag
     6841 cgccgcctgt caagggtggc tttacccgcg ccgatgggtc agccatcgat ccgggccaga
     6901 tacgattcag cgcaggcgaa cgcgtcggcc ttttcgtcga acctgctctg ctcaccgtcg
     6961 tgccgggcaa cagtgtcaca ctgtcgttga tcgtattcaa ccggagcgcg accgacgatc
     7021 gcttcacggt gtcgctgacc ggcgcgccgg ccaattggat ctccgagccg ctgccggagg
     7081 tcgagcttgc gcccggcgcg caggcagaag tgaaacagag catccggcta cctcgctcgc
     7141 cgcaaagccg cgccggacgg cgcgccctca ccgttcgagt ggtcagccag gacgagcctg
     7201 acgaggtcgt cgaagagaga gtgacgctga acatcgtcgt gtattcggag ttcagcagcg
     7261 atctcaggcc gcagcgggtg cgggtggggg agacggcgcg agtcgtcgtg cacaatcatg
     7321 gcaacacgcc ggagaatttc cgcctggcct gcttcgatcc ggcgggcaag ctcgccttcg
     7381 agcctgccga gttgtcgctg acggtgccgg aagggcaggg gattacggcg gaattcatag
     7441 cgggcctgcg ccggcggcgc ctggtcgggg gtacccgggc cgacggcttc ctggcgcgaa
     7501 tagtcgccca gagcggccag gctcagacgc acaccggcga agtcatcagc gccggtctga
     7561 taccggcgtg gatcgtctcg ctcgttttgc tcatcatgtt cgcgggcctg gtagtgggtg
     7621 cggcggcgct ccttctgacg cctcccactc catcgacggc ggcagcgccc gtcatccaaa
     7681 aggccttttc gaatgtgaat ggcctgaact cgaccgtgac gttcacgctc accaacccga
     7741 atgcgacgct cccgctgacg ggggtcgcgt ttacggatac atttccgacg atgcccggag
     7801 cgatggtggt ggctaatccg ccgaacgcga tcgtttcgac gggctgcgga gcgccatcct
     7861 tcgtggcaga tgcaggcagc gggtccatca cttttgtgaa tggcacgatc acggccggtg
     7921 ggacgtgcac gctcgccgtg gacgcgacgg tacctgccgc aggaacttac gacaacacca
     7981 ccgggcctgt gacctcaatc gaaggcggca cgggcgactc tgcctcggcc agcctcatgg
     8041 taagcgctct cgcgccgccg agcatcgcca agtcgttttc gctgacttcg atccctgcgg
     8101 gcgcggtggt gaagctcacc ttcacgattg tgaacccgaa cccggggatc acactgactg
     8161 gcgtgacgtt caccgacgta ttcccggcga cgccaggggc aatggtggtt gccaatccgc
     8221 ccaacccgag catctcggcg gggtgtgggc tgccggcatt tacgccggag gctggcagcg
     8281 gatcgatcac gttctcgaat ggaacgattg caggcggcag tgcgtgtgtc gtaaccatag
     8341 acgtgacagc gccggtggga gggacgtaca cgaacgtgag tggcgcggtc gtgtcggcga
     8401 acggggggac ggggaacacg gcgacggcgg tgttgacggt gggcgcggcg gcgccgccgg
     8461 gcatcgcgaa gtcgttcgcg cccagctcga tcgtggcggg gatgaagtcg acgctgacgt
     8521 tcacggtgac gaacccgaac gcgggggtga cgctgagcgg ggtggcgttc ggcgacacgt
     8581 tcccggcggg catggtcgtg gccaacccgc cgaatccgag cgtgtcggca gggtgcgggc
     8641 tgcctgcctt cacaccggca gcgggcagcg ggtcggtcgc gttctcgaac g
//
LOCUS       DBNHXB010000477         8628 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2841621, whole genome shotgun sequence.
ACCESSION   DBNHXB010000477 DBNHXB010000000
VERSION     DBNHXB010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8628)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8628)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8628
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2841621"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3378
                     /locus_tag="AC162G_18435"
     CDS             <1..3378
                     /locus_tag="AC162G_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077838.1"
                     /translation="ILGQKLPIGLGPEGEIIYNQVPATGTLTFNVNAWWQGYGTLNSS
                     GQPQLYTRTIEYRKGTNKFHDPATGAILNVNPLTVTLPALPPNTQLNVPIVYTVEGAV
                     PKSGYLVPGYGTFVSFADFAGNPAVTFTNLSPLKVSFKHDPLKFGSLVSVTFSLDGQN
                     KGTFQTIGDPDCAGATNYYFNLTNAHQLSAGWHIAHIQAHAAGGSVTNRFFGLYFAKP
                     PDWFYNASYKNRKVSLWSAGFVDMSGEQLPTGSPGSSSTLGADVPRVGYLDNAAGADS
                     RIWQRIKATGDIQTFHYGGVHSKALNTSAPDQKTNQPAAGNNPITFSSGGPITILDTG
                     WMPLFRDVWGIWPIASATIGADMAFNATLTYNGTLTLQPPASTLFVDPEATVAVDAWF
                     DLSALFGVVSANAHAIPAITVGMPVTFVNGVNDDSDICFRYKLDITWSAKVGVCPLCD
                     KESGTKNIFNDYTPKSALCAPGAQSAARAVSTPPSDASSSLATDGFGHSLSVWSDDAG
                     SLRYSTYNGAAWQVAQTLVADGASLKPKVAYFAPNQAVAVWTHNNLTLQQAQTANITD
                     TVKAQHLRYAEWNGSSWSTPQNLTLPATGEGNVALAGCLSTTPGCPSGGAVTAAWVRD
                     VAGALSQQQFRLYYATYQNGAWSAVQAVDAASAATDGEPALVYENGAPLVVWVRDADR
                     NPGTLTDRRLAYRRLDGASPVVVPGDLPAGIVEPSAAVDPSGNLKLAFTLAEDPNAFT
                     GNQRTLHSAAQACTPTCAWSVQKLQDNHGRALRGESPILTLDKNGKGTITFRGLGFGP
                     LPNGQYQAFPEDAIGMVTLTGEAAQVDVDFATTVHSPHYLSNDGAVNWHPSAVYDPAL
                     NQIHTIAVKGAVPVLPLALKAQHSAAVNLRVVHTTQVADEVAFAAVPLQPDFVVTALT
                     PSKRYPELIDPISVTVQLRNDGVAWQASRTEQLDLAALWDGDPGVGTLAASAQITELL
                     GGQIITATLNLTPPVDLDVTHSLLVVVNPLQAVAEQSAENNRQTTLIGGMPVVTDVVA
                     LAQTGNSLVYLQWTPLTDRRVAGYRVYRAEDGGPFVPVGSTFVEGFVDLTASLDHTYR
                     YAVTTFTIDGIESELSSALEVATSSYHVYLPVVLKGH"
     gene            3564..6158
                     /locus_tag="AC162G_18440"
     CDS             3564..6158
                     /locus_tag="AC162G_18440"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5077839.1"
                     /translation="MELLERESHLDALSTALHEARAGNGCIALVSGEAGIGKTVLVEH
                     FTRAQQNTSRVLWGACDALFTPRPLGPLHDMAAQAQSDLLARLTSESNRSVLFSTFLS
                     ELQNQATIVVFEDVHWADEATLDLLKYLGRRVARTSTLVVLTYRDDEVGLRHPLRALL
                     GDLSTSNAVVRIPLSPLSKDAVRALVGERAMDAAALHRQTGGNPFFLTEVLSSAGHGI
                     PATVRDAVLARAARLSLSGQAVLQAAAVIGPRIEPAVLAEVTGAEASAVEECLDLGML
                     VTQAATLAFRHELARQTILETISPLQKLALHRMTLDALRALPTARHDLTRLTHHAEAA
                     GDREAILEFAPAAARQAVAALAHRAAAALFELAFRHADDLPPAERAQLLDEFSVECDI
                     TDRRPEAIEARRQAVALWHAAGQPLQRGRGLSRLALLLQITGQKAEAEEANRAALEIL
                     EPLAPNLELVAAYNMEAWLSLANGDSARGVAMAEKGIAMAQHFEEEKELPPLLEIAGL
                     CWLYLDHARGTEYLERSLTLAHKFGHATRAGNIYANLSSIYVDFHEFTRAEAIFAQGL
                     PFARERDLDAVRAYMEGWLAIHKMHRGDWAAAEEIGREATQRSNASPGHGPALIALGR
                     LRTRRGEPEAMAALDEALDLLLKQGFRQREGMIRAARAEAAWQAGDRGRALEEARAAL
                     DLAVSHHQEWYVGELAFWIWRAGEAVDLPEWAAKPYALHIAGDWRAAAEEWERLGCPY
                     EQARALADGDTDAQIAALELFERLGARPAAEDLRQRMRAAGVSHIPRRPRAATRENPF
                     GLTARQLEILLLLTEGLSNAHIAARLHLSPKTVDHHVSAVLAKLDVHSREEAAALARQ
                     HALFKPIA"
     gene            6160..>8628
                     /locus_tag="AC162G_18445"
     CDS             6160..>8628
                     /locus_tag="AC162G_18445"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5077840.1"
                     /translation="MELLERDAPLRELEAAWKQAAAGEGRVALVSGEAGIGKTALVER
                     FTRDHRDTARVLWSACDALFTPRPLGPLHDIAAQIQGDLLARLNSDASRAMIFSTVLV
                     ELQRTATIVVIEDIHWADEATLDLIKFLGRRLQRTRALLIATFRDDELGLQHPLRFVL
                     GDLATSTATRRFSLAPLSLEAVRALVGRRAFDPEALHRRTGGNPFFIGEVIASGASGI
                     PPTVRDAVLARAARLSASAGAVLEAAAVIGLRIEPWLLADVTGAEAQAADECLAAGML
                     LAQGELVAFRHELARQTILDTIAPHRRMVLNQLVLHALRSSPATRSDLARLAHHAQAS
                     GDREAVLELAPAAARQAAAGSAHREAAALYALALRFANDLPPADHAQLLEAYAGECVS
                     IDQREEGLEAQKKALELWRQLGVPLKQGQSLANIAWSLNGLGKTAAALEASRQAIEIL
                     EAQPPSRELARAYRMQAGLENLCQNYRAGIEWAQKAIALAELFEDQSTVLSAHIAVGA
                     AWLVLDYEDGRQYLERNLRAARHAGQEHLAALIYTNLSCVSSEVYQFQYAERYLMEGM
                     AHAAEHGLEIFHLYMLAWQALTHLRLGRWNEAAGAAGAVLGRPGVSVTSRITALAALG
                     YLRTRRGDPNAHVVLDEALDLSKEIESLHRIGLVRAARAETAWQAGDRDQTLQEARAV
                     YDVAVSKEHPWFTGELAFWRWRAGDEVRLPEWTARPFALHIAGDWRAAAEEWERLGCP
                     YEQARALADGDAAAQTAALKIFERLGARPAADALRQKMRTAGVRAPRKPRASTRENPL
                     GLTHRQVEILRLLTEDLSNAEIAAR"
BASE COUNT         1565 a         2844 c         2785 g         1434 t
ORIGIN      
        1 atattgggac agaagctccc gatcgggctt ggccctgaag gcgagatcat ctacaaccag
       61 gttccggcca cgggcacgct caccttcaac gtcaacgcct ggtggcaggg ctacggcacg
      121 ctgaacagca gcgggcagcc gcagttgtac acgcgcacga tcgagtaccg caagggcacc
      181 aacaagttcc acgatccggc gaccggcgcc atcctcaacg tgaatccgct caccgtcacg
      241 ctgccggccc tgccgccgaa tacacaactg aacgtgccga ttgtgtacac ggtcgagggc
      301 gcggttccca agagcggcta tctcgtgcca ggttatggga cgttcgtatc gttcgccgat
      361 ttcgccggca atcccgccgt gacgttcacc aacctttccc cgctcaaggt gagcttcaag
      421 cacgatccgc tgaaattcgg ctcgctcgtc agtgtaactt tttcgctcga cgggcagaac
      481 aagggaactt ttcagaccat cggcgatccg gattgcgcag gcgcaacgaa ttactacttc
      541 aacttaacca acgcccacca gctatcggca ggatggcaca tcgcgcacat tcaggcgcac
      601 gcggcgggtg gctcggtgac gaatcgcttc tttgggctgt atttcgccaa accgcctgac
      661 tggttctaca acgccagtta caagaatcgc aaagtgtcgt tgtggagtgc aggatttgtc
      721 gatatgagcg gcgagcagct ccccaccggc agcccaggca gcagcagcac gctaggggcc
      781 gacgtgcccc gggtcggata cctcgacaat gccgcgggcg cggatagccg aatctggcag
      841 cgcatcaagg ccacgggcga cattcagaca tttcattacg gcggcgtgca cagcaaggcg
      901 ctgaacacga gcgcgccgga tcagaaaacc aatcagcccg ctgccggcaa caatcccatc
      961 accttcagca gcggcggccc gatcacgatt ctggatacgg gctggatgcc gctcttccgc
     1021 gacgtgtggg gcatctggcc gatcgccagc gcgacgatcg gcgcggacat ggccttcaac
     1081 gcgacgctga cttacaacgg cacgctcacg ctgcagccgc cggccagcac gctgttcgtc
     1141 gatcccgaag cgacggtcgc cgtcgatgcc tggttcgacc tcagcgcgct cttcggcgtg
     1201 gttagcgcga atgcgcatgc gataccggcg atcaccgtcg gcatgccggt gaccttcgtc
     1261 aacggcgtca acgacgacag cgacatctgc ttccgctaca agctggatat cacatggagc
     1321 gccaaggtgg gcgtgtgccc gttgtgcgac aaggagagtg gcaccaagaa catcttcaat
     1381 gactacacgc ccaagagcgc attgtgcgcg ccaggcgcgc agagcgccgc gcgggccgtc
     1441 tcgacaccac catctgacgc gtcctcgtcg ctggcgacgg atggcttcgg ccattctctt
     1501 tccgtgtgga gtgacgacgc tggcagcctt cgatacagca cttacaacgg cgcagcgtgg
     1561 caggtagcgc agacgctcgt cgccgacggc gcgagcctca aacccaaggt cgcttacttc
     1621 gcgccgaatc aggccgtcgc ggtgtggacg cacaacaacc tgacgctgca acaggcgcag
     1681 acggcgaaca tcaccgatac cgtcaaggcc cagcacctcc ggtatgctga gtggaacgga
     1741 tcgtcgtggt ccacgccgca gaatctcacg ctgccggcga cgggcgaagg taacgtcgcc
     1801 ctcgccggct gcctgagcac cacgccgggc tgcccgagcg gcggcgcagt cacggctgcc
     1861 tgggtgcgcg acgtggccgg cgcgctgtca caacagcagt tccgcctgta ctatgccacg
     1921 tatcaaaacg gcgcgtggag cgcggtgcag gccgtggacg ctgccagtgc tgcgaccgac
     1981 ggcgagccgg cgctggtata cgagaacggc gcgccgctcg tcgtgtgggt gcgggatgcc
     2041 gatcgcaacc ccggcacact caccgatcgg cggctggctt atcgccggct cgatggtgcc
     2101 agtcccgtcg tcgtgccggg cgatctgccg gccggtatcg tcgaaccgtc ggctgcagtc
     2161 gatccatctg gcaacctgaa gctcgcgttc acgctggccg aagacccgaa tgccttcacg
     2221 ggtaatcagc gcacgctgca cagcgcggcg caagcgtgca cgccgacgtg cgcgtggagt
     2281 gtacagaaat tgcaggacaa tcatgggcgg gcactgcgcg gcgaatcgcc gatcctgacc
     2341 ctcgacaaga atggcaaggg caccatcacc ttccgtggac tcggctttgg gccgttgccc
     2401 aacgggcagt atcaagcctt ccccgaagac gcgatcggca tggtcacgct cacgggtgaa
     2461 gcggcgcagg tcgatgtgga cttcgccacc accgttcaca gtccgcatta tttgtccaac
     2521 gacggcgcgg tgaactggca tccgtcggct gtgtacgatc cggcgttgaa tcagattcac
     2581 actatcgcgg tgaaaggtgc cgtgcccgtc ctgccactgg cgctgaaggc gcagcatagc
     2641 gcggcggtca acctgcgcgt cgtgcacacg acgcaggtag cagacgaagt agcgtttgcg
     2701 gccgtgccgc tgcaacccga tttcgtcgtg acggccctca cgccgtccaa gcgctatccc
     2761 gaactgatcg accccatcag cgtcacggtg caactgcgca acgacggtgt ggcatggcag
     2821 gccagccgca ccgagcaatt ggatctcgcg gcgctgtggg acggcgatcc gggcgtgggg
     2881 acgctcgccg cgtcggcgca aataacggag ttgctggggg gacagatcat cacggccacg
     2941 ttgaatctga cgccgccggt cgatctcgac gtgacgcact cgctgctggt ggtggtgaac
     3001 ccgttgcagg cggtcgccga gcaaagcgcc gagaacaacc ggcagacgac actcatcggc
     3061 ggcatgccgg tggtgaccga tgtcgtggcg ctggcgcaga ccggcaactc gctggtgtac
     3121 ctgcagtgga cgccgctgac cgatcggcgc gtcgccggct atcgcgtcta tcgcgcggag
     3181 gatggcggcc cctttgtgcc ggtcggcagc accttcgtgg aaggattcgt cgatttgacc
     3241 gcctcgctcg atcacaccta tcgttacgca gtcaccacgt tcacgatcga cggcatcgag
     3301 tctgaactca gcagcgcgct ggaagtggcg acgagcagtt accacgtgta cctgccagta
     3361 gtgctgaagg gacattaacg ggtgattgca cacaagtcag tttaaccact gagacacgga
     3421 gttcgcggag aaatcagaag gccttcctcc gtgccctccg tgtctccgtg gtgaaatggg
     3481 gataatcaca ggacgctaaa ccttgccggg gaagaaatgc ggccggattg acttccacat
     3541 gaggaacctc tataatcgac cggatggagc tgttggagcg tgagtcgcac ctcgacgctt
     3601 tgagtaccgc gcttcacgag gcgagagcgg gcaatggctg catcgcgttg gtcagcggcg
     3661 aagccgggat tggcaagacg gtacttgtcg agcacttcac gcgagcacaa cagaacacga
     3721 gccgtgtgtt atggggcgcg tgcgacgcgc tgttcacccc ccgcccgctc ggcccgctgc
     3781 acgatatggc ggcgcaagcc cagagcgacc tgctcgcccg actcacctca gagtcgaacc
     3841 gctccgtcct cttctcaacc ttcctgagtg aactccaaaa ccaagcgacc atcgtcgtct
     3901 ttgaagacgt gcactgggcc gacgaggcga cgctcgacct gctgaaatat cttggtcgcc
     3961 gcgtcgcccg cacgtcgacg ctcgtcgtgc tgacgtatcg tgacgacgaa gtcggtctgc
     4021 gccacccgtt gcgcgccctg ctcggcgacc tgtccacctc aaacgccgtg gtccgcatcc
     4081 cgctatcgcc cctctccaaa gacgctgtgc gtgcgctcgt cggtgagcgg gcgatggatg
     4141 ccgccgcgct ccatcgccaa accgggggca atcccttctt ccttaccgag gtgctttcca
     4201 gcgcgggcca cggcatcccg gccaccgtgc gcgatgcggt gctggcccgc gcggcgcggc
     4261 tctctctctc cgggcaggca gtgttgcagg ccgccgccgt catcggcccg cgcatcgagc
     4321 cggcggtgct ggctgaggtc accggcgcgg aggcctcggc cgtcgaagag tgtttggacc
     4381 tcggcatgct cgttacacaa gccgcaacgc tggctttccg ccacgagctg gcgcggcaaa
     4441 ctatcctcga gacgatttca ccgctgcaaa agttggcgtt gcatcgtatg acgctcgatg
     4501 cgttgagagc cctacccacc gcccgccacg acttgacgcg gctcacccac catgccgagg
     4561 ccgccggtga ccgcgaagca attttggagt tcgccccggc agcggcccgg caggcagtcg
     4621 cggcgctcgc ccaccgcgct gccgcagcgc tcttcgaact ggcgtttcgc catgccgatg
     4681 atctgccgcc ggcggagcgc gcgcaattac tggatgaatt ttcggtcgaa tgcgacatca
     4741 ccgatcgccg gcccgaggcc atcgaggcgc gccggcaagc cgtggcgctg tggcacgcag
     4801 ccgggcaacc tctgcagcgc ggccgaggtc tcagcaggtt agccctcctg ctgcaaatca
     4861 ccgggcaaaa agccgaagca gaagaagcca atcgcgcggc gctcgagatt ctcgaaccgt
     4921 tggcgcccaa tctcgagctg gtcgcggcgt acaacatgga ggcctggttg agcctggcca
     4981 acggcgacag cgcgcgcggc gtggcgatgg cggagaaggg catcgccatg gcccagcact
     5041 tcgaagaaga aaaggagctg ccgccgctgc tcgaaatcgc aggcctgtgc tggctgtatc
     5101 tcgatcatgc gcgtggcacg gagtatttgg aacgcagcct caccctcgct cacaaattcg
     5161 gtcacgccac gcgggccggc aacatctacg ccaacctcag ctcgatctac gtggacttcc
     5221 acgagttcac gcgggccgaa gcaatctttg cgcaagggct gccgtttgcg cgtgaacgcg
     5281 acctcgacgc ggtgcgggcc tacatggaag gctggctggc gattcacaag atgcatcgcg
     5341 gtgattgggc agcggccgaa gagataggcc gggaggccac gcagcgaagc aacgcctcgc
     5401 ccggacacgg gccggcattg attgcgcttg gccgtctgcg cacgcgacgc ggcgagccgg
     5461 aggccatggc tgccctcgac gaggcgctcg atctgttgct caagcaaggc tttcgccaac
     5521 gcgaggggat gattcgcgcc gctcgcgccg aagcggcgtg gcaggcgggt gatcgcggac
     5581 gtgcattgga agaggcacgc gccgcgttgg atctcgcggt cagccatcac caggaatggt
     5641 atgtgggcga actggctttt tggatttggc gcgcgggcga agcggtggat ttgcccgaat
     5701 gggcggcgaa gccttacgcg ttgcacatcg ctggcgattg gcgcgcggcc gcagaggagt
     5761 gggaacggct gggctgtccc tacgagcagg cccgcgccct cgccgatggc gacaccgacg
     5821 cgcagatcgc cgcgctcgaa ctcttcgaac ggctcggcgc gcggccggca gccgaagact
     5881 tgcggcagag gatgcgcgcc gcgggcgtgt cgcatatccc acgcagaccg agagccgcca
     5941 cccgtgaaaa tcccttcggc ctcacggctc gccaattgga aatcctgctc ctcctcaccg
     6001 aaggcttgag caacgctcat atcgccgcgc gcctgcatct ctcgcccaag acggttgacc
     6061 atcacgtgtc ggcggtttta gctaaactgg acgttcactc tagagaagaa gccgccgcgc
     6121 tcgcccggca acacgccctc ttcaagccca tcgcgtagca tggaactgct cgaacgcgac
     6181 gcccccctgc gcgaattaga ggcggcgtgg aaacaggccg ccgccggcga gggccgggtg
     6241 gcgctggtgt ccggcgaagc cggcatcggc aagacggcgc tcgtcgagcg gttcacacgc
     6301 gatcatcggg acaccgcccg tgtgctgtgg agtgcctgcg acgccttgtt cacccctcgc
     6361 cctctcggcc cgctgcacga catcgccgcc cagattcagg gcgacctgct ggcgcggctc
     6421 aactcagacg ccagtcgcgc gatgatcttc tcaacggtgc tggtcgaact tcaacgcaca
     6481 gccaccatcg tcgtcatcga agacatccat tgggcagacg aggcgacgct cgacctcatc
     6541 aagttcctcg gccgccgcct tcaacgcacc cgcgcgttgc tgatcgccac cttccgcgat
     6601 gacgagcttg gcctgcaaca cccgctgcga ttcgtgctgg gtgatctggc gacctctacg
     6661 gcaacccgcc gcttctcgct tgcgcccctc tcgctggagg cggtgcgcgc gctcgtcggc
     6721 cggcgggcgt tcgatccgga agcgcttcac cggcgcaccg gcggcaatcc cttcttcatc
     6781 ggcgaagtca tcgccagcgg ggcaagcggc atcccgccca cggtgcgcga tgccgtgttg
     6841 gcgcgagcgg cgcgactgtc cgcttcggcc ggggcggttc tggaagctgc cgccgtcatc
     6901 ggcctgcgca tcgagccgtg gctgctagcg gatgtgacgg gcgcggaggc ccaggccgcc
     6961 gacgagtgcc tggccgcagg catgcttctg gcgcaagggg agttggtcgc ctttcgccac
     7021 gagctggcgc gacagacaat cctcgacacc atcgcgccgc atcgtcgaat ggtgttgaat
     7081 cagttggtgc tccacgcgct gcgctcatca ccggccacgc gaagcgacct ggcccggctg
     7141 gctcaccatg cgcaggccag cggggatcgc gaggccgtgc tggaacttgc ccccgccgca
     7201 gcgcggcagg ccgccgccgg gagcgcgcac cgcgaagcgg ccgccctgta cgcccttgcc
     7261 ctgcgcttcg ccaatgatct gccgcctgcc gaccacgcgc aattgctgga agcgtacgcc
     7321 ggggagtgcg tctcgattga tcaacgagag gaaggcctcg aggcgcagaa gaaggctttg
     7381 gaactctggc gccagcttgg cgttccgctg aagcagggcc agagtctggc gaacatcgcc
     7441 tggtcgctta acggtctcgg aaaaactgcc gcggcgctgg aggccagccg gcaggccatc
     7501 gaaatcctcg aagcgcagcc gcccagccgg gaactggcgc gcgcctaccg gatgcaggcc
     7561 ggcctggaaa atctctgcca gaattatcgc gccgggattg aatgggcgca gaaggcaatc
     7621 gccctggccg aattgttcga ggaccagtcc accgtgttgt cggcccacat cgccgttggg
     7681 gcggcctggc tggtgttgga ttacgaggac gggcgccaat acctggaacg gaatctgcgg
     7741 gcggcccgcc acgccgggca ggagcacctg gcggcgctca tctacacaaa cctgagctgc
     7801 gtatccagcg aggtctatca gtttcaatat gccgagcgtt acctgatgga gggcatggcc
     7861 cacgccgccg agcacggttt agagatcttt catttataca tgctcgcctg gcaggcgctc
     7921 acgcatctgc gcctgggccg ctggaacgag gccgccggcg cagccggcgc cgtgttgggg
     7981 cgccccggcg tgtcggttac cagccgcatc acggcgctgg cagccctggg ctatctacgc
     8041 acgcggcgcg gtgatccgaa tgcacacgtg gttctcgacg aggcgctcga cctgtcgaaa
     8101 gagatcgaaa gccttcatcg catcgggttg gtgcgtgcgg cgcgggccga gacggcgtgg
     8161 caggccggcg accgcgatca aacgctgcaa gaagcccgcg ccgtatacga cgtggccgtg
     8221 agcaaggagc atccctggtt cacaggcgag ctggccttct ggcgctggcg ggcgggagat
     8281 gaagtgaggc taccggagtg gacggccagg cccttcgcgc tgcacatcgc cggcgattgg
     8341 cgtgcggcgg cagaggaatg ggaaaggctg ggctgtccct acgaacaggc ccgcgcgctg
     8401 gccgatggcg acgccgccgc gcagactgcc gcgcttaaga ttttcgagcg gctcggcgca
     8461 cggcctgccg ccgatgcgct gaggcaaaag atgcggacgg cgggcgtgcg cgccccgcgt
     8521 aagccgagag cctccactcg cgagaaccct ttgggtctca cccaccggca ggtagaaatc
     8581 ctgcgcctgc tcaccgaaga cttgagcaac gccgaaatcg ccgcgcgg
//
LOCUS       DBNHXB010000478         8917 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1499506, whole genome shotgun sequence.
ACCESSION   DBNHXB010000478 DBNHXB010000000
VERSION     DBNHXB010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8917)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1499506"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            449..2197
                     /locus_tag="AC162G_18450"
     CDS             449..2197
                     /locus_tag="AC162G_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HIR5077841.1"
                     /translation="MSTITITQLISPYGGRLLDLTVPPETLDEVKAYANRLPSIQLTE
                     RAVCDLELLAIGAFSPLDRFMGQADYQSVLDNMRLDSGHIFPVPVTLSVEPDPAIQLG
                     RDVALRNSKNELLAVMTIEEIYSWDLREAAQKVFGTQDLRHPLVAEMHGWGRLHISGR
                     LQVIQLPRRYDFRDLRLTPAETRARLEAFGYPNVVAFQTRNPLHRVHEELTKRAAQEV
                     DGVLLLHPVVGLTKPGDVDHYTRVRTYKALAQRYYDPGRILLALLPLAMRMAGPREAL
                     WHAVIRRNYGANHLIVGRDHASPGVDSTGKPIYGPYDAQELVARFSDELGVAMVPFRE
                     LVYLPEENRYEEAHRVDQHVQTASISGTQVREDYLNNGRKLPDWFTRPEVADILAETY
                     PPRHRQGVCIWFTGLSGAGKSTTAEVLTVLLLEHGRQVTMLDGDVVRTHLSKGLGFSK
                     DDRDVNIRRIGFVASEIARHGGVVMCAAVSPYRATRNDVRNMVGKDRFVEVFVDTPID
                     VCEERDAKGMYAKARRGEIKDFTGIDDPYEPPLHPELRLETISSTPEQNAHLILDYLV
                     RQGFVRIAAPMMEHTN"
     gene            complement(2418..3644)
                     /gene="coaBC"
                     /locus_tag="AC162G_18455"
     CDS             complement(2418..3644)
                     /gene="coaBC"
                     /locus_tag="AC162G_18455"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR5077842.1"
                     /translation="MDPIPLLKDKRILLGVSGGIACYKTVDLASQLTQAGALVDCVLT
                     ESATKFVSPLTFAAVTGRPALTDADLWRSDLHVPHVGMGHAAHLIVVAPATANTIARL
                     AHGLSDNLLTVSVLAAKCPMIVAPAMDAGMWEHPATHANVEILRSRGAHIAGPAEGRM
                     ASGLIGLGRMVEPEELLGWIRVVLGRQGALSGRPVVVSAGGTREAIDPVRFVGNHSSG
                     RQGFALAQAAIDRGAGVTLVAAAAALATPVGAKRIDVESAQEMHDAVLEACRRADALI
                     MAAAVADFRPERTVDQKIKTKDTPDLDLRLVRTTDILQAVKRQRDATKLPKVVIGFAA
                     ETRDLIENARAKLDSKGLDLIVANDVSAKDAGFAVDTNRVTLLDAGGGVESLPVMSKA
                     QVAERVMDRLVALLRG"
     gene            complement(3816..4685)
                     /locus_tag="AC162G_18460"
     CDS             complement(3816..4685)
                     /locus_tag="AC162G_18460"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5077843.1"
                     /translation="MSEYTWWSGGQVEYLDTRDGRVAYRFWPESRDAKKTLLGIHGLG
                     GNSDNYAALGEALKPDVAVYAIDLAGNGESGTRGDVESLEVHMRNLDTLANLIQARHP
                     DAEHFVAGYSLGAAYAPAWVARNGQAVSGMILFAPPFRTVFHIPRVLDIAFRTLSVLM
                     PRYRVPTGIRAEDGLDPRYEFEMESDKFIRARTLRSLKVSADMVPLAERALPGITIPT
                     LIVHGDADSVAFPESAELAHARLGAVDKTLVWIPRAKHDLHDVLSGVKCSEVSDDQRA
                     PVIAAVREWLHRH"
     gene            complement(4802..5590)
                     /locus_tag="AC162G_18465"
     CDS             complement(4802..5590)
                     /locus_tag="AC162G_18465"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR5077844.1"
                     /translation="MLLALDVSNTTIKAGVFRGEKLVADWRIATERHKLADEYAMLML
                     SLFQASGMPVSEITGVAVSCVVPPLRSVFDELARRYLHVEPLIVGPGIKTGVRLAVDN
                     PREVGADRVVNVLATHRLYGGPAITIAFGTATVFDCVSAEGDYLGGAIAPGIVVALES
                     LAQSAAQLYQVELVRPPRAIGKNTVHTMQSGLVLGYAGLVEGLVKRLRREMAGTNADA
                     KVTVIATGGLAEVIAPETDAIDITDLQLTLKGLRLIYELNKPGD"
     gene            complement(5793..6644)
                     /gene="panC"
                     /locus_tag="AC162G_18470"
     CDS             complement(5793..6644)
                     /gene="panC"
                     /locus_tag="AC162G_18470"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR5077845.1"
                     /translation="MQLVTTIEDFRKAKSALHGTWGFVPTMGYLHEGHLSLVRRAKAE
                     NDRVAVSIFVNPTQFGPSEDLAAYPRDLQRDLRLLEPLGVDLVFHPAPEALYPPDFQT
                     YVTVEEVTKRLEGAARPGHFRGVATVVTKLFNVVQADRAYFGQKDAQQVVVIRRMVVD
                     LDMPIRIVVCPTVREPDGLAMSSRNAYLDPAQRNAAAVLFRALSAAKARCDAGERDAE
                     AIRGAMRDTLEKEPLAKTEYVSAAAPLTLRELDRVEDDVLLSMAIRIGKTRLIDNFLK
                     EGGAWFS"
     gene            complement(6718..8490)
                     /locus_tag="AC162G_18475"
     CDS             complement(6718..8490)
                     /locus_tag="AC162G_18475"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR5077846.1"
                     /translation="MRFSHHFGSTLREAPAGVEAVSHQLLLRAGFIRQLGQGLFSYLP
                     LAHRSLTKIENIMREEMNAIGGQELTMPVAHPAEVWQATGRWYSVGPEMARFKDRKDR
                     DMVLAMTHEEVVADLCKTEIKSYRQLPQLIYHIQTKFRDDPRPRAGLIRVREFTMKDS
                     YSLDLDEVGLDRQYRAHYQAYFNIFNRCGVAAIAVGADVGMMGGSQAHEFMYLTPIGE
                     DTLVLCRQCGYAANRQIARFAKPAAAPEDLRPIEKVPTPDTRTIEALATLLGVPKSKT
                     AKAVFMIAGHEEDALRETREEFVFAVVRGDMEVNETKLGNAVKADWLRPASDDEIRAA
                     GAAPGYASPIGLKAVTVVVDDAVPASPNLVAGANEEGYHLLNTNTPRDYAASIVADIA
                     AAGDGDACIQCGQPLVTARGVEIGNIFKLGTRYSDVIGATYLAADGAQKPIVMGSYGI
                     GSGRLLACVAEEYHDDKGLIWPVTVAPYHVHLVSLAGAEARVLETAERVYRALLDANI
                     EVLYDDRDESPGVKFADADLLGVPIRLTVSARSLGKGGVEMKRRDRKELEIVAESDLI
                     ARAQAEIESMLEEIQAKVVEMPFK"
     gene            complement(8643..>8917)
                     /locus_tag="AC162G_18480"
     CDS             complement(8643..>8917)
                     /locus_tag="AC162G_18480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR5077847.1"
                     /translation="PYADPFERARQVAQATATNRSSMLQDVLKGRPTEIDAINGKIVE
                     RGRAVDIPTPINATLAALVRALERTPTAESAESAKRAMSQVLNGRT"
BASE COUNT         1544 a         2942 c         2780 g         1651 t
ORIGIN      
        1 ggagcagcgt cgaaaagcgc aacgagaatt tctccatcag ctcgttcggc gaggacgcgg
       61 acggcgagct gctcgtgctc gactattccg gcggccgcgt gctcaggctc actgtgaaca
      121 cctatcgtat ccatctaccg atgatcaaca agagcaactg attctaccct ccgctgcgca
      181 cggcagcgcc atagttgacc tcgtctcctc cgtttgttca caccaacctc tgcgcagctt
      241 accttattct aacgagtctc cccccttttt ttaaccgcca tatgctaccc ttgctccgta
      301 tggagcccgg aggggtatgt tgcttttcac gtgacccgtt cacgtagctc agatttggcg
      361 acgcgaccct tcggcttcgc tcagggcagg cccttcggca acatacggtc aagagttgac
      421 gaaaatcact agatcgaggt catagaaatt gagcaccatc actatcacgc aactgatttc
      481 tccgtacggc ggcaggctgc tggacctgac cgtcccaccc gagacgctgg acgaagtgaa
      541 ggcgtatgcc aaccggctgc cttcgattca actcaccgag cgggcggtat gcgatctcga
      601 actgctcgcc atcggcgcgt tctcgccgct ggatcggttc atgggccagg ccgattatca
      661 gagtgtgctg gataacatgc gcctggatag cggccacatt ttccccgtgc ccgtgacact
      721 gtcggtcgaa cccgatccgg cgattcaact gggacgagac gtggcgctgc ggaactccaa
      781 gaacgaactg ctggcggtca tgacgatcga ggagatatac agttgggatc tgcgcgaggc
      841 ggcgcagaag gtgttcggca cgcaggactt gcggcacccg ctggtggccg aaatgcatgg
      901 ctggggcagg ctgcacatct ccggtcgctt gcaggtcatt caattgcccc ggcgctacga
      961 cttccgggac ttgcgcctca caccggccga gacgcgcgcc cggcttgagg cgtttggcta
     1021 tccgaacgtc gtcgcgttcc agacgcggaa tcccttgcat cgcgtgcacg aagagctgac
     1081 gaagcgagcc gcgcaggaag tcgacggcgt gctcctgctg catccggtcg tcggcctgac
     1141 caagcccggc gatgtcgatc attacacccg cgtgcgcacg tacaaagcgc tggcccagcg
     1201 ctactacgac ccggggcgaa tactgctggc gctgctgccg ctggcgatgc gcatggccgg
     1261 gccgcgcgag gcgttgtggc acgccgtcat ccggcgcaac tacggcgcga accatctgat
     1321 cgtcgggcgc gatcacgcca gcccgggcgt ggactcgacc ggcaagccga tctacgggcc
     1381 ctacgatgcg caggagctgg tcgcacgctt cagcgacgag cttggcgtcg ccatggttcc
     1441 cttccgcgag ctggtgtacc tgcccgaaga gaatcgctac gaggaggcgc atcgcgtcga
     1501 ccagcatgtg cagacggcct ctatctcggg cacgcaggtg cgcgaagatt acctgaacaa
     1561 cgggcggaaa ctgcccgact ggttcacccg gcctgaagtc gccgacatcc tggccgaaac
     1621 gtatccgccg cgccaccggc agggggtgtg catctggttc accggcctga gcggcgctgg
     1681 caagtccacg accgccgagg tactgaccgt gctgctcctg gagcatggcc ggcaggtgac
     1741 gatgttggac ggcgacgtcg tgcgcacgca tctgtccaaa ggactgggct tcagcaagga
     1801 cgaccgggac gtgaacatcc ggcgcatcgg cttcgtggcc tcggaaatcg cgcggcacgg
     1861 cggggtggtc atgtgcgcgg cggtcagccc ctaccgggcg acgcgcaacg atgtgcgcaa
     1921 catggtcggc aaggatcgct tcgtcgaggt gttcgtagac acgcccatcg acgtttgcga
     1981 ggagcgcgac gccaaaggca tgtacgccaa ggcccggcgc ggtgagatca aagatttcac
     2041 cggcatcgac gatccctacg aaccgccgct gcaccccgag ctcagactgg agacgatctc
     2101 gtccacaccc gaacagaacg cgcatttgat cctggactat ctggtgcgac agggttttgt
     2161 gcggatcgcc gcaccgatga tggagcatac gaactgagat cggcacggct gtggggcttt
     2221 gttggcccgt gcggcgagaa ggtgggggaa ccggcgcatg gtgctcaaac cgccggattt
     2281 tcacccgccg cgcgcgggcc aatggagaaa ccaggtttct caaagaaacc tggtttttgt
     2341 caccctcatc cgaacaatcc cggacgcggc gctcgactgg cagccgggcg gcacagactg
     2401 gtcactcagg cagattatca gccccgcagc agcgccacca ggcgatccat cacccgctcc
     2461 gcgacctgcg ccttgctcat cactggcagc gattccacgc cgccccccgc gtcgagcagc
     2521 gtgacgcggt tcgtgtccac ggcgaacccg gcgtccttcg ccgacacgtc gttcgcgacg
     2581 atcagatcga ggcccttgct gtcgagtttc gcccgcgcgt tctcgatcag gtctcgcgtc
     2641 tccgccgcaa agccgattac gaccttgggc agcttcgtcg cgtcgcgctg ccgcttcacc
     2701 gcttgcagga tgtcggtcgt gcgcaccagg cgcaagtcga gatcgggtgt atccttcgtc
     2761 ttgatcttct gatcgacggt gcgctcaggc cggaagtcgg cgacggcggc agccatgatc
     2821 agcgcgtcgg cgcggcggca cgcttcgagc acggcgtcgt gcatctcctg cgccgactcg
     2881 acgtcgatgc gcttcgcgcc gaccggcgtg gccagcgcgg ccgcagccgc gaccagcgtc
     2941 acacccgcgc cgcgatcgat cgccgcctgc gccagcgcga acccttgccg gccgctcgag
     3001 tggttgccca cgaagcgcac cggatcgatc gcctcgcgcg tgccgcccgc cgatacgacg
     3061 accgggcgcc cgctcagcgc cccctgccgg ccgagcacga cgcggatcca gcccaatagc
     3121 tcctccggct cgaccatgcg ccccagcccg atcaggccgg aagccatgcg cccctccgcc
     3181 gggccggcga tgtgcgcgcc gcgcgagcgt agaatctcga cgttcgcatg agtggccggg
     3241 tgctcccaca tgcctgcgtc catcgccggc gcgacgatca tggggcactt cgcggcaagc
     3301 acgctgacgg tgagcaggtt gtcgctcagg ccgtgcgcca gcctggcgat cgtgttggcc
     3361 gtcgccggag cgacgacgat cagatgggct gcatgcccca tcccgacgtg agggacgtgc
     3421 aagtcggatc gccacaggtc ggcgtcggtc agcgcggggc ggcccgtgac ggcggcgaac
     3481 gtcagcggcg agacgaactt cgtcgccgat tcggtgagca cgcaatcgac gagcgcgccc
     3541 gcctgcgtca actggctcgc cagatcgacg gtcttgtagc aggcgatacc gccgctcacg
     3601 ccaaggagaa tgcgcttgtc tttgagaaga ggtatggggt ccatcgataa gtgttcagtg
     3661 gtcagtgttc agtaatcagt tgaagccgcc gacaccttgc cggtccacgt acgccgtcac
     3721 ggcggcgaca ctcatcttcg gcgacgggtt gagcatcgct cactgattac tgattactga
     3781 ttactgatta ctaataactg attactgatc agcaatcagt gcctatgcag ccactcgcgc
     3841 acggctgcga tcaccggcgc gcgctggtcg tcgctcacct cgctgcattt gacgccgctc
     3901 agcacgtcgt gcagatcgtg cttcgcgcgg ggtatccaga cgagcgtctt atccaccgcg
     3961 cccaatcgcg cgtgggccag ctcggcgctt tcaggaaagg ccacgctgtc ggcgtcgccg
     4021 tgcacgatca gcgtgggaat cgtgattccc ggcagggcgc gctcggccag cggcaccatg
     4081 tctgccgaga ccttcagcga gcgcagcgtc cgcgcgcgga tgaacttatc gctctccatc
     4141 tcgaactcgt accgcggatc cagcccgtcc tccgcccgaa tcccggtcgg gacgcggtat
     4201 ctcggcatca agaccgacag cgtgcggaac gcgatatcca ggactcgagg gatgtggaac
     4261 accgtgcgaa agggtggggc gaacaggatc atgccgctga cggcctgccc gttgcgcgcg
     4321 acccacgccg gcgcgtatgc cgcgcccagg ctgtatccgg ccacgaagtg ctcggcgtcg
     4381 ggatggcgcg cctggatgag gttcgccagt gtgtcgaggt tgcgcatgtg cacctcgagg
     4441 ctctccacgt cgcctcgcgt ccccgactcg ccgttgccgg ccagatcgat cgcgtatacc
     4501 gccacgtccg gcttgagcgc ttccccgagc gcggcgtagt tatcgctgtt cccgccgaga
     4561 ccgtgaatgc cgagcagcgt cttcttcgcg tcgcgcgatt cgggccagaa acgatacgcg
     4621 acgcggccat cgcgcgtgtc gagatactcg acttgccctc cgctccacca ggtatattca
     4681 gacatgctcc cccatcagcg ctagagcgga ttccgattgc gtgtacgccc atccggagtc
     4741 gagcctttag gcggttccgg ctgaagcctc gactccggcg taaatcaaac tgggatctgc
     4801 tctagtcgcc cggtttgtta agctcgtata tcagccgcag ccctttcagc gtgagctgca
     4861 ggtcggtgat atcgatcgca tccgtctccg gcgcgatgac ttcggccaat ccccccgtgg
     4921 caatcaccgt tacttttgcg tccgcgttcg tcccggccat ttcgcgtctc aggcgcttga
     4981 ccaggccctc gaccagcccg gcgtatccga gcactaatcc cgactgcatg gtgtggacgg
     5041 tgttcttgcc gatcgctcgc ggcggcctca ccagctcgac ctgatacaat tgcgcggccg
     5101 actgggcgag cgattcgagc gcgacgacga tgccgggagc gatcgcgccg ccgaggtagt
     5161 cgccctcggc cgatacacag tcgaagacgg tcgccgtgcc gaaggcaatc gtgatcgccg
     5221 gtccgccgta caggcgatgc gtcgccagca cgttgacgac gcgatcggcg ccgacctcgc
     5281 gcgggttgtc gacggccagt ctcacgcccg tcttgatgcc ggggccgacg atgagcggct
     5341 cgacgtgcag gtagcgccgc gcgagctcgt cgaagaccga gcgcagcggc gggacgacac
     5401 acgagacggc cacgccggtg atctcgctga ccggcatgcc gctggcctga aacaggctga
     5461 gcatgagcat ggcgtattcg tcggcgagct tgtgccgctc ggtcgcaatg cgccaatcgg
     5521 cgaccagctt ttcgccgcga aacacaccag cttttatcgt cgtattgctg acgtcgagag
     5581 cgaggagcac tttagtccgt tggtcgggtg gtcatgtagt cgagtggtcg ggtagtctga
     5641 tggtcgtgca gtcggatggc cgggtggtca gtcaatcggg tcgtcacgta gtcggatcga
     5701 tgttctaccg gcggccagga ccatcaaacc acgtgaccac gcgaccaacc gaccatgcga
     5761 ccacctgact atctgactac gtgaccacgt gactaagaga accatgcgcc gccttctttc
     5821 aggaaattgt cgatcaggcg cgtcttgccg atgcggatgg ccatggacaa cagcacgtcg
     5881 tcttcgacgc ggtcaagctc tcgcagcgtg agtggagcgg ctgccgatac gtattcggtt
     5941 tttgccagcg gctctttttc cagcgtgtcc cgcatcgcgc cacggatcgc ttccgcgtcg
     6001 cgctccccgg cgtcgcaacg cgccttcgcg gcggacaacg cgcggaagag aacggctgcg
     6061 gcgttccgct gcgccgggtc gaggtacgcg ttgcggctgg acatcgccag cccgtccggc
     6121 tcgcgcaccg tcgggcagac cacgatccgg atcggcatgt ccagatcgac gaccatgcgc
     6181 ctgatcacga cgacctgctg cgcatccttc tgcccgaagt acgcccgatc ggcctgaacg
     6241 acgttgaaca gcttcgtgac gaccgtcgcc acgccgcgaa agtgaccagg ccgcgccgcg
     6301 ccttcgagcc tcttcgtcac ttcttcaacc gtaacgtagg tctggaagtc gggcggatac
     6361 agtgcctcag gcgccgggtg aaacacgagg tcgacgccga gcggctccag gagtctgaga
     6421 tcgcgctgca gatcgcgcgg ataggcggcc aggtcttcac tcgggccgaa ctgtgtcgga
     6481 ttgacgaaga tgctgaccgc gacgcggtcg ttctcggctt tcgcgcgccg cacgagcgag
     6541 agatggcctt cgtgcagata ccccatcgtc ggcacgaaac cccacgtccc gtgcagagcc
     6601 gatttggctt ttcggaagtc ttctatggtc gtgacgagct gcattgcacc tgtgtgcatc
     6661 ttgacctgct tctggagcat ttcgcgtatc gcttatcgcg tatcgcaaac gcatcgctca
     6721 cttgaatggc atttcgacga ccttggcctg aatctcctcc agcatcgact cgatctctgc
     6781 ttgcgcgcgt gcaatcaaat cggattcagc gacgatctcg agctcctttc ggtcgcgccg
     6841 tttcatctcg acgccgccct tgccgagcga cctggcgctg acggtcaggc ggatgggaac
     6901 gccgagcagg tcggcgtcgg cgaacttgac gccgggactt tcgtcgcggt cgtcatacaa
     6961 gacctcgata ttcgcgtcga gcagcgcgcg atacacgcgc tcggcggttt cgagcacgcg
     7021 cgcctcggcg cctgccagcg acacgagatg aacgtgatac ggcgcgacgg tgaccggcca
     7081 gatcaggccc ttgtcgtcgt ggtactcctc ggcgacacag gccagcagcc gcccggaacc
     7141 gatgccgtac gaccccatga cgatcggctt ctgcgcgccg tcggcagcca gatacgtcgc
     7201 gccgatcacg tcggaatagc gcgtacccag cttgaaaatg ttgccgatct cgacgccgcg
     7261 cgccgtaacc agcggctgcc cgcactgaat gcacgcgtct ccatcgccgg ccgccgcgat
     7321 gtcggcgacg atcgacgcag cgtagtcgcg cggcgtgttg gtgttgagca ggtggtagcc
     7381 ttcttcgttt gcgccggcga cgaggttcgg cgaggcgggg acggcgtcgt cgacgacaac
     7441 cgtcaccgcc ttcaacccga tcggcgaggc gtagcccggc gctgcgcccg cggcgcggat
     7501 ctcgtcatcg ctcgccgggc gcagccaatc ggctttcacg gcgttgccga gcttcgtctc
     7561 gttcacttcc atatcgcccc gcaccacggc gaacacgaat tcctcgcgcg tctcgcgcag
     7621 cgcgtcctcc tcgtgaccgg caatcatgaa cacggccttg gccgtcttgc tcttcggcac
     7681 gccgagcagc gtcgcgagcg cttcgatcgt cctcgtgtcc ggcgtgggaa ctttctcgat
     7741 cggcctgagg tcttccggcg cagcggcggg cttggcaaag cgggcgatct gccgattggc
     7801 tgcgtagccg cactggcggc acagcacgag cgtatcctcg ccgatcggcg tgagatacat
     7861 gaactcgtgc gcctggctgc cgcccatcat gccgacgtcc gcgccgaccg cgatcgccgc
     7921 cacgccgcag cggttgaaga tgttgaagta agcctggtag tgcgcgcggt actgccgatc
     7981 cagcccgact tcgtcgagat cgaggctgta tgagtccttc atcgtgaact cgcgcacgcg
     8041 gatcagcccg gcgcgcggac gcggatcgtc gcggaacttg gtctggatgt gatagatcaa
     8101 ctgcggcagt tgccggtagg acttgatctc ggtcttgcac agatcggcga cgacctcttc
     8161 gtgcgtcatc gccagcacca tgtcgcgatc cttgcgatct ttgaagcggg ccatctccgg
     8221 cccgaccgag taccagcgcc ccgtcgcctg ccacacctcg gcgggatgag cgaccggcat
     8281 cgtcagctcc tgcccgccga tcgcattcat ctcttcgcgc atgatgtttt cgatcttggt
     8341 gagcgagcgg tgcgccagcg gcagatacga gaacaggccc tgtcctagtt ggcggatgaa
     8401 ccctgcgcgc aatagaagtt gatggctaac cgcttcgacg ccggcagggg cttcgcgtag
     8461 ggtagaaccg aaatggtgag agaacctcaa agcatatctc ccgggtaccc aaagtaaaac
     8521 gtgaagcgtg aagcgtgaac atcgattcac acttcacgct tgcgtcacgt gcagcctgtc
     8581 aatggtcaga catgctacgc gacattgtag cacaggtcgc agagccacgg cagcttctcg
     8641 gctcaggtcc tcccattcag aacttgactc atcgccctct tggcgctttc cgcgctctcg
     8701 gcggttggag tcctctccaa tgcgcgaacc agcgcggcca gcgtcgcatt gatcggcgtc
     8761 gggatatcga cggcgcgtcc gcgctcgaca attttaccat tgatcgcgtc gatctctgtc
     8821 ggccggccct tgagcacgtc ctgcagcatc gacgagcggt tcgtcgccgt cgcctgcgcc
     8881 acctgccggg cgcgctcgaa cggatcggcg tagggca
//
LOCUS       DBNHXB010000479         7009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1667144, whole genome shotgun sequence.
ACCESSION   DBNHXB010000479 DBNHXB010000000
VERSION     DBNHXB010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7009
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1667144"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="AC162G_18485"
     CDS             <1..423
                     /locus_tag="AC162G_18485"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR5077848.1"
                     /translation="GPAVYDNTLYLGDLSSNLYALDLDGREIWTLPSGTELTPNNPEN
                     KNRLEGMIRAQPLVTEDRIYLATGAGKLYALDRATHSHVWTFTSLQGGEQFLTTPVLV
                     GETLLVAPVPSGASPVYLYAINAVSGNAQWLFPPRQEQ"
     gene            464..1207
                     /locus_tag="AC162G_18490"
     CDS             464..1207
                     /locus_tag="AC162G_18490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIR5077849.1"
                     /translation="MDFLISPLVNLLLIFYSVLGASFPLAIVVMTILVRLVTLPLTLP
                     QQRSMRKMNELQPQLKELQKKYAGNRQKLSEEQMKLYREYGVNPLGGCLPLILQLFIM
                     IAFYQAITSALSVTPLQMLGLSQRVGPNIASLLPLNSQFLWLNLSQPDPFFILPILVV
                     ATTYLQQKMMSPSGADPSQASMTRQMNIMMPLMFGLFALQFASGLSIYFIVSNLVGIA
                     QYLLIGRRQATAAADSKPKPAKAKAAVKK"
     gene            1242..1961
                     /gene="jag"
                     /locus_tag="AC162G_18495"
     CDS             1242..1961
                     /gene="jag"
                     /locus_tag="AC162G_18495"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HIR5077850.1"
                     /translation="METPQSIEAVGASVDEAIERALDALNVTREQVSVDVIEPGGGKT
                     MARVRVTLMTPISAPPAPPAIAPETAGAPAAPEEIRMARETLSALLEHMHIRADVEAY
                     QAPAQDDQDLHAEGGLPLVLNIKGDDLGILIGRRGETLRDLQYLLRLIVGRQAGRNVN
                     LVVDVEGYKTRREQTLRQLALRMAERAVATRKTVMLEPMPANERRIIHLTLRNHPSVS
                     TQSVGQGDGRKVTIVPKSRTP"
     gene            2052..2876
                     /locus_tag="AC162G_18500"
     CDS             2052..2876
                     /locus_tag="AC162G_18500"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR5077851.1"
                     /translation="MLYLAIGHVCQDVVPGGYALGGSVTYATLTARTCGWSVAAITRA
                     SSHLDLSPLGEMEWVRLPSGATTTFENIYTPSGRIQILHATAGPIGPGDVPRHLRRAD
                     VVHLAPIADEVDAALVDVFDGAWIGVTPQGWMRQWDASGRVSRRMWTKAESILARADA
                     TVLGLDDVGGDWDEIERWAAVAHLLVVTQGPAGCTVFERGASAQVPGYPTTEVDPTGA
                     GDIFATAFFTQLRDTGDALLAARFANCVAAHSVTRRGLAGVPTAAEVQQCLAVLNN"
     gene            2877..3665
                     /locus_tag="AC162G_18505"
     CDS             2877..3665
                     /locus_tag="AC162G_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR5077852.1"
                     /translation="MAHIYAFANQKGGVGKTTTAVNLGAYLAASGKRVLLIDLDPQAN
                     ATSSLGIAKSQVEKSIYDSLIGDTPLTDVVQLTKRVHLDLAPSATALSGAEVELVTMP
                     SREKRLRRVVEGVSASYDYLLVDCPPSLGLLTVNALVAAHGVIVPVQCEYLALEGLTR
                     LMQTIGIVRKSLNPRLTVRGMLMTMYDGRTRLSQQVVDEVRKHFPDRVFDTVVPRSIR
                     LGEAPSYGEPILSYAPSSSGGQAYAQLAAELLAADNGQLVPQVP"
     gene            3709..4569
                     /locus_tag="AC162G_18510"
     CDS             3709..4569
                     /locus_tag="AC162G_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR5077853.1"
                     /translation="MSARKKTGLGKGLGALLPETDVTAGLSEAPVGAISPNPNQPRAS
                     IDENDLRELAHSIREHGVIQPLVVARSGENAYTLIAGERRWRAAQLIGLDSVPVVVKD
                     VVPQQMLELALVENVQRADLNPLEEASAYRQLVEEFGLTQEQVAQRVGKSRVAVANTL
                     RLLKLPDAIQKRLADASISEGHARALLMLNDTAHMQRLASRIVSDGLSVRQVEEQVRR
                     LNAGRPQTRKRKASGTVSANTRALEDRMRRALGTKVGLYRSSRGGKIVIQFYSEEELD
                     AIYRRIVQED"
     gene            4716..5909
                     /locus_tag="AC162G_18515"
     CDS             4716..5909
                     /locus_tag="AC162G_18515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR5077854.1"
                     /translation="MSTHPIVHEKVDQAIGVLQEKNIDVWLTFVRETTLMRDPALNLI
                     VSPALELTWHSAFLLTQRGDRIAIVGRFDKENVAGLGDWREVIPFDQGIGKPLLDTLR
                     RLNPQSIAINTSESDPAADGLSHGMWTTLLDILKGTPYTRMISAEDIINALRGRKTAT
                     EISCVKDAIVDTEAIFDEITATLRPGLSEIEIAEFTHDRMQARGLTTAWDYDYCPVVD
                     AGPESPVGHAAPTQLQTQRGRLLHIDFGVTKHGYVSDMQRTWYLLQEGETKPPAEAQR
                     GFDAVRSAILAGADALRPGAKGWEVDRASRSTLINAGYPEYLHATGHHIGQTVHDGAT
                     VLGPRWERYGESINGLVEPGNIFTLELGVHVPDLGFVGLEEDVLVTDGGIEWLSKPQT
                     ELICV"
     gene            complement(6438..6896)
                     /locus_tag="AC162G_18520"
     CDS             complement(6438..6896)
                     /locus_tag="AC162G_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077855.1"
                     /translation="MFRKISAVSFALALVLAFAAPAAADPGQPNFGPAVYGDGQVWGT
                     KGAAGLPAPGESNPQSFDKLFVITNSNAAGQLPVAEAAPGNPNYDGGRWFTHTVTWTQ
                     AGFDAHGTVPVLMSYADILFHYSLGHLTIAAGSPAGGPPPYFECPLLPVK"
BASE COUNT         1328 a         2270 c         2236 g         1175 t
ORIGIN      
        1 gggccggccg tgtacgacaa tacgctatac ctgggcgatc tgagcagcaa cctgtacgca
       61 ctcgatctgg acggccgcga aatctggaca ctgccgagcg gcaccgagtt gacgcctaac
      121 aaccccgaaa acaagaaccg gttagaaggc atgattcgcg cgcagccgct cgtcaccgaa
      181 gatcgcattt acctggcgac cggcgccggc aagctgtacg cgctggatcg ggctacgcac
      241 agccacgtct ggactttcac ctcgctgcaa ggcggcgagc agtttctgac gacgcctgtg
      301 ctcgtcggcg aaacgctgct cgtcgcgccg gtaccctcgg gcgcatcgcc cgtctacctg
      361 tacgcgataa acgcggtgtc gggtaacgcc cagtggctat tccctccgcg tcaagaacag
      421 tgaggcgccg aagacgacgc tgaggggaca caagaggata actatggact ttctcatctc
      481 gccgctggtc aacttgctct tgattttcta cagtgtgctc ggcgccagct tcccgctggc
      541 gatcgtcgtc atgacgatcc tggtgcgtct ggtcaccctg ccgctgacgc tgcctcaaca
      601 gcgatcgatg cgcaagatga acgagctgca gccgcagctc aaggagctgc agaaaaagta
      661 tgcgggaaac cggcagaaac tgtccgaaga gcagatgaag ctgtaccgcg agtacggcgt
      721 caatccgctg ggcggctgcc tgcctctgat cctgcagctt tttatcatga tcgcgttcta
      781 tcaggcgatt accagcgcgc tgtcggtgac tccgctgcag atgctggggc tgtcgcagcg
      841 agtgggaccg aacatcgcgt cgctgctccc gctcaattcg cagttcctct ggctcaatct
      901 cagccagccc gatccgttct tcatcttgcc tatcctggtc gtggcgacga cctacctgca
      961 gcagaagatg atgtcgccct ccggcgcgga tccgagccag gcctccatga cgcgccagat
     1021 gaacatcatg atgcctttga tgttcggcct gttcgcgctt cagtttgcat cgggactgag
     1081 catctacttt atcgtctcga atctcgtggg catcgcccag tacctcctga tcgggcgccg
     1141 gcaggcgacg gccgccgcgg acagcaagcc caagccggcc aaggccaagg cggccgtcaa
     1201 aaagtaggcg gtgactttcc cgcgccgcat tggagatgca cgtggaaaca ccccaatcga
     1261 tcgaagccgt tggcgcctcg gttgacgagg ccatcgagcg cgcgctggac gcgctgaacg
     1321 tcacgcgtga gcaggtcagc gtcgacgtca tcgagccggg cggaggcaag acgatggcac
     1381 gcgtccgcgt cacgctcatg accccgatct cggcgccacc tgccccgccg gctatcgcgc
     1441 ccgagaccgc cggcgcgccg gccgcgccgg aagagattcg gatggctcgc gagacgctgt
     1501 cagccctgct ggagcacatg catattcgcg ccgacgtcga ggcgtatcag gctccggctc
     1561 aagacgatca ggacctccac gcagagggcg gactgcccct ggttttgaac atcaaaggcg
     1621 acgacctggg catcttgatc gggcggcgcg gcgagacgct gcgcgatctg caatacctct
     1681 tgcgcctgat cgtcggccgg caggccggac gaaacgtcaa cctcgtcgtc gacgtggaag
     1741 gctacaaaac ccggcgcgag caaacactga ggcaactggc gctgcgcatg gcggagcgcg
     1801 ccgtcgccac gcgcaagacg gtcatgctcg agccgatgcc ggccaacgag cgccgcatca
     1861 tccatctgac actgcgcaat cacccctcgg tctccacgca aagcgtgggc cagggcgacg
     1921 gccgcaaggt cacgatcgtt ccgaagtcgc gcacgccgtg agaccaggta tgcaggcccg
     1981 gtttcgcgcg cgaaaccggg cctgtctttc tccttgaaca aatcgcggtt cttcgttaca
     2041 atcacagcgt catgctctac ctggcgatcg gtcacgtgtg ccaggacgtc gtgcccggtg
     2101 gatacgccct cggcggcagc gtcacatacg cgacgctgac ggcgcgcacg tgtggctggt
     2161 cggtcgccgc gatcacacgc gccagttcgc atctcgacct gtcgcccttg ggtgaaatgg
     2221 aatgggtgcg cctgcccagc ggcgccacga cgaccttcga gaatatctac acgccatccg
     2281 gccggataca gattttgcac gccaccgccg gaccgatcgg ccccggcgac gttccgcgcc
     2341 atctccgccg cgccgacgtc gtccacctcg ccccaattgc agacgaggtg gatgcagcgc
     2401 tcgtcgatgt gttcgacgga gcatggatcg gcgtcacgcc ccaaggctgg atgaggcaat
     2461 gggacgcgtc aggccgtgtc tcgaggcgca tgtggacgaa ggccgagtcg atactggccc
     2521 gcgccgacgc gacggtgctg ggcctcgacg acgtgggcgg cgactgggat gagatcgagc
     2581 gctgggctgc cgtcgcccac ttgctcgtcg tcacgcaagg ccctgcaggc tgcacggtgt
     2641 tcgagcgagg cgcatcggcc caggttcccg gttacccgac gaccgaagtc gatccgacgg
     2701 gcgcgggcga catcttcgcg acggcgttct tcacgcagtt gcgcgacacc ggcgacgcct
     2761 tgctcgccgc acgctttgcc aactgtgtgg ccgcgcattc cgtcacgcgg cgcggtctgg
     2821 ccggcgtgcc gacggcggcc gaggtgcagc agtgtctggc tgtgctcaat aactgaatgg
     2881 cacacatcta cgccttcgcc aatcaaaaag gcggcgtcgg aaagacgacc acggcggtga
     2941 acctgggcgc ttatctggcc gcgagcggca aacgcgtcct gttgatcgat ctggatccac
     3001 aggcgaacgc cacgagctcg ctgggtatcg ccaagagtca agtcgagaag tcgatctacg
     3061 actccctcat cggcgacacg cccttgacgg acgtcgtcca gttgacgaaa cgcgtgcatc
     3121 tcgacctggc gccgtcggcg acggcgctgt caggcgctga agtggagctg gtgacgatgc
     3181 cgagccgcga gaagcggctc agacgcgtcg tggaaggcgt gtcggccagc tacgactact
     3241 tgttggtcga ctgcccgccg tcgctgggac tgctcactgt caacgcgctg gtcgccgcgc
     3301 acggcgtgat cgtgccggtg cagtgcgaat accttgccct ggagggcctg acccgtctga
     3361 tgcagacgat tggcatcgtg cgcaagtcgc tcaatccgag gctgaccgtg cgcggaatgc
     3421 tgatgacgat gtacgacggg cgcacccgac tctcgcagca agtcgtggac gaagtgcgca
     3481 aacattttcc ggaccgcgtg ttcgacaccg tcgtcccgcg cagcatccgg ctgggtgaag
     3541 cgccgtcgta cggcgagccg atcctgtcgt atgcgccgtc gtcgtccgga ggccaggcct
     3601 acgcgcaact ggccgccgaa ttgctggcag cggacaacgg ccagttagtt cctcaagttc
     3661 cttgagtccc atgagcagga acttaaggaa ctcaggcgaa acgacacgat gagcgccagg
     3721 aagaaaaccg gactcggcaa gggactcggc gcgctgctgc ctgaaaccga cgtcacggcc
     3781 ggcctgagcg aagcaccggt cggcgcgatc tcgccgaacc cgaaccagcc gcgcgcatcc
     3841 atcgacgaga acgatctacg cgagctggcc cactcgatcc gcgagcatgg cgtgattcag
     3901 ccgctggtcg tcgcgcgctc cggtgaaaac gcctatacgc tgatcgccgg cgagcggcgc
     3961 tggcgagcgg cccaactgat cgggctggac agcgtgccgg tggtggtgaa ggatgtcgtg
     4021 ccccagcaga tgctcgagct cgcgctcgtc gaaaacgtcc agcgcgccga cctcaatccg
     4081 ctcgaagagg cgtcggcgta ccggcaactg gtcgaggagt tcgggctgac ccaggaacag
     4141 gtggcgcagc gcgtcggcaa aagccgcgtc gccgtggcaa ataccctgcg cctgctcaag
     4201 ctgcctgatg cgatccagaa gcgtctcgcc gacgcgtcga tcagcgaagg ccacgcgcgc
     4261 gcgctgctca tgctcaacga tacggcgcac atgcagcgcc tggcgagccg gatcgtgagc
     4321 gacggtttga gcgtgcgcca ggtcgaagag caggtgcgcc gcctgaacgc gggcaggccg
     4381 cagacccgga agcgcaaagc gtccggcacc gtctccgcaa acacgcgagc gctggaagat
     4441 cggatgcgcc gcgcgctcgg caccaaagtc ggtctttacc gctccagtcg cggtggcaag
     4501 atcgtcatcc agttctattc ggaagaggag ctagacgcaa tctaccgccg tatcgttcag
     4561 gaagattagt gccttgccaa tcaacttcat cttttccagc aaagatttgc cacggagaca
     4621 cggagacact gagttctctc tgtgtctctg tgcctcagtg gcttttatcc acctctcggg
     4681 ttccggcttg tccgggttag ggagaaaggc ggcgaatgtc cacccatccg atcgttcatg
     4741 agaaagtcga ccaggccatc ggcgtgctgc aggagaagaa catcgacgtg tggctgacct
     4801 tcgtgcgcga gaccacgctg atgcgcgatc cggcgctgaa tttgatcgtc agcccggcgc
     4861 tggagctgac ctggcattcg gctttcctac tcacccagcg aggcgaccgc atcgccatcg
     4921 tcggccgctt cgacaaagag aacgtcgccg gcctgggcga ttggcgcgag gtgatcccct
     4981 tcgaccaggg catcggcaag ccgctgctcg acacgctgcg ccgcctgaac ccgcagagca
     5041 tcgccatcaa cacgagcgag agcgacccgg ccgccgatgg cctctcgcac ggcatgtgga
     5101 cgacgctgct cgacatcctg aaagggacgc cgtacacgcg aatgatctcg gccgaagaca
     5161 ttatcaacgc gctgcgtggg cgcaagaccg ccaccgagat ctcctgcgtc aaggatgcta
     5221 tcgtggatac cgaggcgatc ttcgacgaga ttacggccac cctgcggccc ggcctcagcg
     5281 aaatcgagat cgccgaattc actcacgatc gcatgcaggc acgcgggctg acgacggcct
     5341 gggactacga ttactgcccg gtcgtggacg ccggtcccga atcgccggtc ggtcacgccg
     5401 cgccgacgca gttgcagacg cagcgcgggc gcctgctgca catcgacttc ggcgtgacga
     5461 agcacggcta cgtgtccgac atgcagcgca cctggtatct gctgcaagag ggcgagacga
     5521 agccgcccgc cgaagcgcag cgcggattcg acgcggtgcg cagcgcgatc ctggccggcg
     5581 cggacgcgct ccgtcccggc gcgaagggct gggaagtcga tcgggcgtcg cgctccacct
     5641 tgatcaacgc cggctacccg gagtaccttc acgcgaccgg ccaccacatc ggccagacag
     5701 tgcacgacgg cgcgacagtg ctcggcccgc gttgggagcg ctacggcgag tcgatcaacg
     5761 gcctggtgga gccgggcaat atcttcacgc tggagcttgg cgtgcacgtg ccggacctgg
     5821 gtttcgtcgg gctggaagaa gacgtgctgg tgacggacgg cgggatcgag tggctgagca
     5881 agccgcagac cgagctgatc tgcgtgtaac tctggacgca cctgtcacac ggccgcccga
     5941 cccgggaggg ctgaattgga aatctcaacc gcttcccgct gttcatgcgc cgatttccac
     6001 ccaggcccga acccaccgcc ggcgagccac cggctgcggc gaccatcgcg caacccagct
     6061 ccgaggcgcc tgccccagcc gaggcaagcg cactcgtcga gccagccgcc tcggcgatca
     6121 tcgagcagcc acgcccgtac acactccggc cggacgtcac gccaaaacag gcgctcctgg
     6181 cggcagcagc cgtggccaga tctttccgat cggcctggcg ttgggctggc tatattggcg
     6241 ctacggcgca gaaagcagca tgctggcgca cgagcttttc aacgtgctag ggctgctcct
     6301 cacccctacg ctgatgacgt gacccagacc gaatagacct ggcaggtctt cgagacctgc
     6361 caggtctgtg gctcaggatt tctagatgca gccgccccag acggccggcg gcaatgtgta
     6421 caaagaacgg cgcgctacta cttcacgggc aaaagagggc attcgaagta aggcggtggc
     6481 cccccggcag gcgagccggc ggcgatggtg aggtgcccga ggctgtagtg gaacaggatg
     6541 tcggcgtaag acatcagcac cggaacggtt ccgtgagcgt cgaagccggc ctgcgtccag
     6601 gtcaccgtgt gtgtgaacca gcggccgccg tcatagttcg ggttgccggg ggcggcctca
     6661 gccacgggta attgtcccgc cgcgttgctg ttggtaatga cgaagagctt gtcgaacgac
     6721 tggggattgc tttcgcccgg cgccgggaga ccagccgctc ccttggtccc ccagacctga
     6781 ccgtcaccgt acacggccgg gccgaaattg ggctggcccg gatcggcggc ggcaggcgcg
     6841 gcgaatgcca gcacgagagc gagcgcgaaa gagacggctg agatcttgcg aaacatggct
     6901 ttcctcctga aagagattgg atggtcggga agtgaacctc ccgtatagca tcagcgtaca
     6961 acttcgatgt tacaaggcac ttccgaggag attacgcatt tattacaac
//
LOCUS       DBNHXB010000480         5035 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1500501, whole genome shotgun sequence.
ACCESSION   DBNHXB010000480 DBNHXB010000000
VERSION     DBNHXB010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1500501"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3645)
                     /locus_tag="AC162G_18525"
     CDS             complement(<1..3645)
                     /locus_tag="AC162G_18525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HIR5077856.1"
                     /translation="MTENRTTWLDQPVSSVLRLDWEKTLYIALFVLAVLTRFWDLGAR
                     AISHDESLHAYYSWELYRGQGFSHTPLMHGPLRFHLNALFFALFGADDFTVRIPTAIS
                     GVMLVMAPILLRRWLGRRGALVTSFFLLISPMLLYHARYIRDEPFMALYAILMAWVSL
                     SYLHDRRSKWLIVLAMLVALMYTTMEASFIYIAVFGLFVVFIAVFEVAREAGWGRHGV
                     AGSLLGIGAALVLFGIGLLIGARLIGAIRFEPAVDGGPSLSSVLLSLLVIGVFGVLIA
                     AGVYSVLRALMPAAARRSAGLDLAIILGGLSLLMLSSSPLSVLNYGALYERVGDNFRV
                     MADAGTTTVWGLLSGEGTTVFVDPYFFAGGNFPTDPANAMNVARLAFLFVCFTALAMG
                     IGLWWSARIWTVALGIFGGIGIVLFTTVFTNGIGLGTGFVGSLGYWLAQQAVSRGTQP
                     TGYHFFIGIMYEYLPMLLSVVALLYYGSRYVRGRSVMVVGTTKPDLSDHVAVPLFMTL
                     VVLLWAGFTVAGEKMPWHMTHLAMPMILLSGRLVGDWLDRIDWRGVAERRTWLALVLF
                     PIAFLAIGAVLASLGAARAAQPGVAAPSLTQLNAWSGLLSGLLIGGGALAALVMLTRR
                     SGMRPIVSMIGLAALVLLTLMTVHTSWSYTYINYDYPTEFGVYAHGSPGLKIALDQIR
                     KLSERVTGTPTEIELLYDSESTWPWLWYLRDFPNKRLIASAPSRGDMTLPVMILYSDN
                     WAAADQAAGNSYNSYEFQRIWWPMEDYKRIPDIVCPREVTRKDGSVERYAAYDENGDS
                     QIDASEEAAGTARCLARMPTLLSALWDIFFRRDYTQYADLTGQNMTLQDWPLRAEFRL
                     YVRRDLAAKVWDQAIGDLAASSSVAPNPVASDPYPSRWQELAASQVIGSAGSDEGQFV
                     SPHGVAIAPNGSIYVADSNNHRVVKFDGDGRFVKAFGTWSGEPPNGDFFNPDWNPPPG
                     TFYEPWDVAAAPDGSVYVADLWNSRIQKFDEDGNFVTMWGGFGDSGQAARGTEGRFYG
                     PRGVAVGEDGLVYVSDTGNKRVQVFDGDGKFVAQFGGGGLLDGNLDEPVGIAVTGDGE
                     IVVADTWNGRVQVFSADGQPLRKWDIAGWLDYTQADFGRSKVGKPYLTVGPDGRIYVS
                     DQVGNRILVFDASGNYLASFGRFGADDVGFTAPSGLAIDADGNILVVDTGNGRVMVFP
                     PLDTGEEDDEL"
     gene            complement(3857..>5035)
                     /locus_tag="AC162G_18530"
     CDS             complement(3857..>5035)
                     /locus_tag="AC162G_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077857.1"
                     /translation="PQRMTILLVFAVAYVLSATGGLTRPSGLAAAGDLVAAWFGAFAA
                     SGGMPVAGILQILIVYEPLAMLIGVAGLGRVLVRRGQREAPVIDPETGQRTEQPPFDE
                     LALEEPGARFGVWLGFTVIAALAIVLIQAGRQPIDLLLPVTLLALLGAYAIQAWAEMM
                     IAEASPGVESVLLAVGAAAAAFLMLTLTTFVRRQVMPTLFAGAGSLAQFGLIGLFLLV
                     LGAGAGLMTLLYGGRAVLRAGSTLALGLLALASLSAAWGATQVRAGDAREIIWGPEVT
                     TPGVFLLLDTADQLAIRSQGHLESLPIRVETDDPVVLWYLRDARVLPDQAPAGMVTLY
                     GEQPQSVTGSYIGARFNIRESWDVTGLNPDAWLKWALYRESDDNRPVPTQSLTLWQRQ
                     "
BASE COUNT         1053 a         1726 c         1475 g          781 t
ORIGIN      
        1 cagttcgtcg tcttcctccc cggtatcgag tggaggaaag accatgacgc ggccgttgcc
       61 cgtgtctaca accaggatgt taccatccgc atcgatcgcc aggccgctcg gcgcagtgaa
      121 acccacatcg tccgcgccaa accggccaaa cgacgccaga taatttccgg acgcgtcgaa
      181 gacgaggatg cgatttccaa cctgatccga gacgtaaatg cgaccgtccg ggcctacggt
      241 caaatacggc ttgccgactt tggagcgacc gaaatccgcc tgggtgtaat caagccaacc
      301 ggcgatatcc cacttgcgca gcggctgccc gtcggcgctg aagacctgaa cccggccatt
      361 ccacgtatcg gcgacgacga tctcgccatc gccggtcaca gcgatgccga ccggctcgtc
      421 gagattcccg tcgagaagcc cgccgccgcc gaactgggcg acgaacttgc cgtcaccgtc
      481 gaacacttgc acacgcttgt tgcccgtgtc ggacacgtag acgaggccat cctcgccgac
      541 ggcgacgccg cgcgggccgt agaagcggcc ctcggttccg cgcgccgcct ggcccgaatc
      601 gccgaatccg ccccacatcg tgacgaagtt gccgtcttca tcgaatttct gtatacggct
      661 attccacagg tcggccacgt acaccgagcc gtccggcgcg gcggccacat cccacggctc
      721 gtagaaggtg ccgggcggcg gattccagtc cggattgaag aaatcgccgt tgggcggctc
      781 gccgctccac gtgccgaatg ccttcacgaa gcgcccgtcg ccgtcgaact tgacgacgcg
      841 atggttgttc gagtcggcga cgtaaataga gccgttcggc gcaatggcca cgccgtgcgg
      901 cgagacaaat tgcccctcgt cgctgcctgc cgatccaatc acctggctgg cagccaactc
      961 ctgccagcgc gacgggtaag gatcgctggc caccgggttc ggtgcgacgc tgctgctcgc
     1021 ggccagatcg ccgatcgcct ggtcccagac cttggccgca aggtccctgc gcacgtacag
     1081 gcggaactcc gcccgcagcg gccagtcctg aagcgtcata ttctggccgg tcaaatcggc
     1141 gtactgggtg taatcgcgac ggaagaatat atcccacaag gcagacaaca gcgtcggcat
     1201 acgggcgaga cagcgtgccg tgccggcagc ctcctcactc gcgtcgatct ggctgtcgcc
     1261 gttctcgtcg tacgcggcgt agcgctcgac gctgccatcc ttccgggtca cctcgcgcgg
     1321 gcagacgatg tcgggaatgc gcttgtaatc ctccatgggc caccagatac gctggaactc
     1381 gtacgagttg taactgttgc cggccgcctg atcggcggcg gcccagttgt cgctatacag
     1441 gatcatcacc ggaagcgtca tgtccccgcg cgacggcgcg ctggcaatga gacgcttgtt
     1501 gggaaaatcg cgcaggtacc acagccacgg ccaggtggac tcagaatcgt aaagaagctc
     1561 gatctcggtc ggcgtgccgg tcacgcgctc ggacagcttg cgtatctggt cgagcgcgat
     1621 cttcagcccc ggcgagccgt gggcgtacac gccgaactcg gtcgggtagt cgtagttgat
     1681 gtacgtgtag ctccacgacg tgtgcacggt catcagtgtc aagagcacca gcgccgcgag
     1741 gccaatcatg ctcacgatcg ggcgcatgcc ggatcgccgc gttagcatga caagcgcggc
     1801 gagcgcgccc ccgccgatca gcaggcccga cagcaggccg ctccacgcgt tgagctgcgt
     1861 gaggctgggc gcggccacgc ccggctgggc ggcgcgcgcc gcccccagcg aggccagcac
     1921 ggccccgatg gccaggaacg caatcgggaa caagacgagc gcgagccacg tgcgtcgctc
     1981 ggcgacgccg cgccagtcga tgcgatccag ccagtcgcca accagtcgtc cactcagcag
     2041 gatcatcggc atcgccaaat gcgtcatgtg ccacggcatc ttctcgccgg ccacggtgaa
     2101 tccggcccac agcagcacga ccagcgtcat gaacagcggt acggccacgt gatcgctcag
     2161 atcgggcttc gtggtaccga ccaccatcac cgaacgcccc cgcacgtagc gcgagccgta
     2221 atagagcagc gcgacaaccg acaacaacat aggcaggtac tcgtacatga tcccgatgaa
     2281 gaagtgataa cccgtcggtt gagtgccacg actcacggct tgctgagcca gccagtaacc
     2341 cagcgagccg acgaagcccg tgcccaagcc gatgccgttg gtgaacacgg tcgtgaataa
     2401 gacgatgccg atgccgccga agatgcccag cgcgaccgtc cagatgcgcg cactccacca
     2461 caatccgatt cccatcgcca gcgccgtaaa gcacacgaac aggaacgcta gacgcgcgac
     2521 attcatggcg ttggcgggat cggtcgggaa gttgccgccg gcaaagaagt aggggtcgac
     2581 gaagacggtg gtgccctcgc cgctgagcag accccacacc gtcgtggtgc cggcatcggc
     2641 catcacgcgg aagttatcgc ccacgcgctc gtacagcgcg ccgtagttca atacggatag
     2701 cggcgacgac gacagcatga gcagcgacag gccgcccaga ataatcgcca aatccaaccc
     2761 agccgatcga cgcgcggcgg ccggcatgag cgcgcgcagc acggagtaca ccccggcagc
     2821 gatcaaaacg ccgaacaccc cgatcacgag cagactgagc agcaccgatg acagcgaagg
     2881 cccgccgtcc accgctggct caaatcggat ggcgccgatc agacgcgcgc cgatcagcaa
     2941 gccgatgccg aaaagcacca gtgccgcgcc gatacccagc agcgatccag ccacgccgtg
     3001 acgcccccag cctgcctcgc gcgccacctc gaacacggca atgaacacga cgaagaggcc
     3061 gaacacggca atgtagatga atgacgcctc catcgtcgtg tacatgagtg ctaccaacat
     3121 cgccagcacg atcaaccatt tggaccgtcg atcatgcaag taggacaaac tcacccatgc
     3181 catcagaatc gcgtacagcg ccatgaatgg ctcatcgcgg atgtagcgag cgtgatacag
     3241 cagcatcggc gagatcagca ggaagaacga cgtcaccagt gcaccccgcc ggcccaacca
     3301 gcgcctcagc aagatcggag ccatcaccag catcacaccc gaaatcgccg tcggaatgcg
     3361 cacggtaaaa tcgtccgcgc caaacagtgc aaagaacaac gcgttcagat ggaaacgcag
     3421 cgggccgtgc atgagcggcg tatggctgaa cccctgcccg cgatacagtt cccacgaata
     3481 gtaggcgtgc aggctctcgt cgtggctgat ggcgcgcgcg cctaagtccc agaagcgcgt
     3541 tagcacggcc agcacgaaga gcgcaatgta cagcgtcttc tcccaatcga gacgcaggac
     3601 gctcgacacc ggctggtcca gccacgtcgt tcgattttcc gtcatcactt tattcacact
     3661 gacctcgctt gtcgcaaagc gcgtatcgcg tatcgcatat cgcgtatcgc ttattgccga
     3721 tcgcatatcg caaaggatat atggcatcgc gtttcacgcg ctcgctccac gtttcaggct
     3781 ccacgtttta cgctccacgt tttacgtttc acgttttacg cttcaccttc cacgcttcac
     3841 cttccgcgct ccacattcat tgtctctgcc acagtgtgag cgactgtgtc ggaacgggtc
     3901 tgttgtcgtc gctctcgcga tacagcgccc acttgagcca ggcatccgga ttcagccctg
     3961 tcacatccca cgattcacga atgttgaatc gagcgccgat gtagctgccg gtcactgatt
     4021 ggggctgctc accgtagagc gtgaccatgc cggccggcgc ctgatccggc agcacgcgtg
     4081 catcgcgcag gtaccagagc acaacaggat catccgtctc gacccggatg ggcagggatt
     4141 ccagatggcc ttgcgatcga atcgccagtt gatcggcggt gtccagaagc aagaaaacgc
     4201 ccggcgtcgt cacctctggc ccccagatga tctcccgcgc atcgccggcg cgcacctgcg
     4261 tcgcgcccca tgccgcgctc agcgatgcga gcgcaagcag gcccaacgcc agcgtcgagc
     4321 cggcgcgcaa aacggcgcgc ccgccataga gcagcgtcat caacccggcc cccgcaccga
     4381 ggacgagcag gaacaggccg ataaggccga actgggcaag cgagccggcg cccgcaaaga
     4441 gggtgggcat gacctggcgc cgcacgaacg tggtgagcgt caacatcaag aacgcggccg
     4501 cagccgctcc cactgccaga agcacactct ctacgccggg cgaagcctcc gcgatcatca
     4561 tctcggccca tgcctgtatc gcgtaggcgc ccagcagtgc tagcagcgtg accggcagca
     4621 ataaatcaat aggttggcgg cccgcttgaa tcaagacgat cgccaatgcg gcgatcaccg
     4681 tgaagcccag ccacacgcca aaccgtgcgc caggctcttc cagcgccaac tcgtcgaagg
     4741 gcggctgctc cgttctctgt ccggtctccg gatcgatgac cggcgcctcg cgctgcccgc
     4801 gcctgaccag cacacgcccc aggccggcga cgccgatcag catggccagc ggctcataca
     4861 cgatcagaat ctgcagaatg cccgcgacag gcataccgcc gcttgccgca aacgcgccaa
     4921 accacgcggc caccaggtcg ccggccgcag ccagtcctga cgggcgcgtc aatccgcctg
     4981 tcgccgagag aacgtacgcc acggcaaaca cgagcagtat cgtcatccgc tgggg
//
LOCUS       DBNHXB010000481        11521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2673923, whole genome shotgun sequence.
ACCESSION   DBNHXB010000481 DBNHXB010000000
VERSION     DBNHXB010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11521
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2673923"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="AC162G_18535"
     CDS             complement(<1..200)
                     /locus_tag="AC162G_18535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077858.1"
                     /translation="MTEPTADRLALLYRITQTFNSSLDLNEVLNCVIDEVIAATRAGR
                     GFVMLLGDDGSMDFRAARGIDHR"
     gene            633..3734
                     /locus_tag="AC162G_18540"
     CDS             633..3734
                     /locus_tag="AC162G_18540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR5077859.1"
                     /translation="MADSSHDDETRRLDADEGETRRIDSDETRQIDPKGNPTVHSKRA
                     ETRRMSGWASDPTGSDATRTGHTPSPPPPPPASGGRGERIEPAPQGGWRASPDAAPPP
                     PPAYPRSGRRAGGKAPAPAPAARVAARGGQVRQPAFWPSFARTLFIMAILAGVAFTTV
                     LAVGAGGYVYIASQLPSPEDLSQRATELFTSSQIYDRDGNLLFELLDPQGGRRTVVEL
                     DHMSKWVRQATIDTEDPRFYRHPGFDPIGIVRAVIQNLREGETVSGASTIAQQLVRNL
                     MLPPEERAERTANRKIKEAVLAAEVTRRYPRDKILELYLNSVYFGNLAYGVEAAAQTY
                     FQKDAEDLTLAEGSFIAGLVQAPSVYDPYTPDGREAALARHRTVLRLMVEAGSITREQ
                     AGAAAEEMAAYEFKPPRVAVEIPSPHFVQHVRQIVETHFGPEALYRGGLKIYTTLDPE
                     LQSIAEDVVQEQIAGLADRNVYGGALVAMEPNTGRVLAMVGSPDYRDEQHAGQINMAV
                     APRQTGSAIKPLNYLAAFEKGWTPSTAIWDTPVVYTDTAGNVYRPVNYDGRFHGPQPV
                     RSALANSYNVPAVKTLEFVTVPEFLKVAQRFGITTFTRPDYGLSLTLGGGEAPLIEMA
                     GAFQIFANGGVRRPPVVIERITNVRGDVQCQFALPGQDAGGAPPCESAPGAGEQVISP
                     QHAYLITHILSDNLARCPAFGCPNILEIGRPAAVKTGTTNDFRDNWTIGYTPELVTGV
                     WIGNPDRSEMKNISGVTGAGPIWHNFMQRALEGQPVLEFPHPPGIIEMEVCADSGTQP
                     SPYCPARRTEIFAADRPPLGPEHDWYQLVRLDAASGLRWVEGCTSGVVEKVMAVVPPE
                     GFEWAQQHGLEVAPIETCAAGPTASIVAIGSPPDGGFVDGVVPVIGTATIPDFESYDL
                     QFASAVSPDTWRWISGPHLAQVVDGELTQWDTNGLEDGEYILRLVVHALSGGSTEARV
                     RVFINRGAIPTPTDTPGPTATPTPAPTETPVVVPTLPPSTETPMSEPTLPPADTVEPT
                     PTP"
     gene            complement(3925..4809)
                     /locus_tag="AC162G_18545"
     CDS             complement(3925..4809)
                     /locus_tag="AC162G_18545"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5077860.1"
                     /translation="MQRLFARRPPAPAPLDVRPATKADRGALARLTEQSRRTHFHLDW
                     WSIDDWLAEPSSNAWVAVAGNPIAGAVIAPANELPVAWVRLIAIADGYDARAVLEALF
                     AAALPPLRAAGTGSLACLAYPDWLTDYLPDLGFTPFTDVTHFRKDDNSIPEQGSPAPT
                     LRQASPGDLPAVLANDRVAFDPIWWHTLDSLARIQGDAAHFIVAELDGRIVGHAFSDV
                     YVGRGHLIRLAVHPGYQRRGIGTRLLAEELTYLLAAGAYPVTLNTQANNFTSQTLYRR
                     FGFAPTGESTTVMLRQID"
     gene            5043..6092
                     /gene="recA"
                     /locus_tag="AC162G_18550"
     CDS             5043..6092
                     /gene="recA"
                     /locus_tag="AC162G_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR5077861.1"
                     /translation="MPSDGKAKALDTALATISKKFGDGAIMRLGEASHLEIAAIPTGS
                     IALDLALGVGGVPRGRITEIYGPEASGKTTICQHVVAEAQRMGGTCAYIDMEHALDPS
                     YAARCGVDVDNLYVSQPDTGEQALQIADALIHSNAIDVVVIDSVAALVPRAEIEGEMG
                     DSHMGLQARLMSQALRKLSGAIKASNTAMLFTNQLRMKIGVMFGNPETTSGGMALKFY
                     ASVRLDVRRIQTIKAEGEAAGSRVRVRVTKNKVAPPFREAEFDIMYNEGISKTGDLLD
                     VGTTLDIVTKRGAFFTYGDTKLGQGREKAKEFLKQNPEITTQIDRDIRTHAAEAAQLT
                     RLGSSAGGGEDESEE"
     gene            6542..7207
                     /locus_tag="AC162G_18555"
     CDS             6542..7207
                     /locus_tag="AC162G_18555"
                     /inference="COORDINATES: protein motif:HMM:NF046475.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HIR5077862.1"
                     /translation="MGRAITALVAQKKNKDRVNVYLDGKFAFGLAAIEAVKLRRGQVL
                     SDEDIARLRALDDAEKAYDRALHFLSFRPRSEAEVRRNLDEAGFAPETAVAAIGRLKR
                     AGLLDDRAFAQYWLENRGQFSPRSSRALKAELRRKGIDLSTIDGALQAAAHDDDEAAY
                     RAALPRARRLAHLDARAFAQKLGAYLARRGFGYDVALEAVRRAWEEVRPNAPDSSFET
                     SED"
     gene            7211..8740
                     /gene="rny"
                     /locus_tag="AC162G_18560"
     CDS             7211..8740
                     /gene="rny"
                     /locus_tag="AC162G_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR5077863.1"
                     /translation="MELGSIVLGVIAGLIAGAALGYTLYRYRQQQQGLHARAEAERLL
                     DEAQTKAKELELKGHDEAIRIRDEAEKEAARRRADLQREEERLARKREQLDEKLEKTE
                     QRERRLNQRQSQLDKQANDLAKVEQQRTAEMARIAQMTVEDARRELLVAVEKTARDDM
                     ARVIRQVEAEIKEEADRRAREIVTLAIQRIAAEAGGDVAVSVVPLPNDEMKGRIIGRS
                     GRNIRAIEKATGVDIVVDDTPEAVTLSSFDPVRREVARVTLTNLIQDGRIHPARIEKL
                     VEDARAEVDRVIKEEGERATIEAGVPGVHPEVQKMLGRLKFRTSYGQNQLYHAIETAR
                     LAGIMAAELGADVKVCKAGGLLHDLGKAMDHEVEGSHALIGADFARRHGVDLRVVNCM
                     ASHHHEVEAESLEAVIVEAADAISGSRPGARRESIELYLKRVKDLEDIASSFEGVAQS
                     YAIQAGREIRIIVRPEDIDDLASLQLSKNIARKIEETMQYPGQIKVTVIRETRAVDYA
                     K"
     gene            8888..10390
                     /locus_tag="AC162G_18565"
     CDS             8888..10390
                     /locus_tag="AC162G_18565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HIR5077864.1"
                     /translation="MRAITVLCLFLILTACTGQGQVTGEPGAPTEMPARLPVQPTATS
                     EPATETPAKPAAQPAAIPSFESSVLLAQWNANERRHEILPVDPATGQAVPGQAPLIIG
                     KISQFAGVQTLSSDGRWLAAIESHGQVSEPNGGGTSHYSSADVLHLVDVTAWRAVTTT
                     LPGRGWVWPITFSPDTARLALGYHNRTSSTLMLFDTGTGQLIAQQALAFRPSLMKYTQ
                     EGTALAIYGQPLSAVPGIAKPDPPRVLLVDAATLDILWDQPLASVISGSWCLENCDAP
                     HGEQMFAGWWPAVVPSHDGHTLYIVHADEEKLTAVDFDARTVHSVEIREARSWFEELL
                     ALTAGVAEAKGGENGAFKEAVLSPDGTRLYVVGRTVNSTRDARGEWQVTEAFLGLQVI
                     DVESGRKVTGLDGQAKAIGTTPDGAYLLLDDWDGRVWWTEVLDAESLQRVARLERWAV
                     VPARRIDGQPILLASDPYRGSQFAVLDPRSFDIVDTWSVSEHAEWVTTSP"
     gene            complement(10527..>11521)
                     /locus_tag="AC162G_18570"
     CDS             complement(10527..>11521)
                     /locus_tag="AC162G_18570"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR5077865.1"
                     /translation="GARLGRYAVHITMLMLAVIAVGMSAAPRVDSVAAGILSASIAQP
                     LPQPSFELFATRGFFASNGNSVSRLADLHTSIPYRTRRDIVVYVVQPGDTVQGIAAAY
                     GLNPETIMWANPAVEDLPDLLRIGQEIVVLPIDGVYHEVKEGDSLQSIAAKYKVDVDA
                     ITGAANEWNQLPAPDYTITPGMKLIVAGGSKPYVPKLVTAYNGPIPTGARGTGRFQWP
                     VVGRITQDYWYGHRAVDIAAPTGTPVYAADGGFVSFVGWTDIGYGYLIRIDHGNGFAS
                     WYAHLSGFNVVLGQAIRRGDLIGTVGSTGHSSGPHLHFEIRTVEGLINPRFYLP"
BASE COUNT         2194 a         3665 c         3844 g         1818 t
ORIGIN      
        1 cggtggtcga tgccgcgcgc cgcgcgaaag tccatagacc cgtcgtcgcc gagcagcatg
       61 acgaagccgc gcccggcgcg cgtcgccgcg atcacctcgt cgatgacaca attcaaaacc
      121 tcgttcaggt cgagcgacga gttgaaagtc tgcgtgatgc gatacagcag cgccaatcga
      181 tcggcagtcg gctccgtcac ggtctgtccc acgtctatag agtcaaggtt tgcttttgaa
      241 attcgcccaa cgtgcgacgc aatttggaag tgcgtcgcac gtcactgtca ggcgaggctc
      301 gttggctgcg ccgattatac ttcacaatgg gccgcaacgt tcaatgccgc gactttcagt
      361 cgcgcatgtt cgccaccaga ctgagatgca cgcggagtgg ccagcgtcgg gcgcccggcg
      421 cgcacccgta ggctgcgcgc agttgacact ccggctctcc tcattataat caggcgtgac
      481 gcccggggga cgccgaacgc gaccgggcgt cttcgatcgg gagctggtga ttacccacaa
      541 gtgctcgggc cgcagtcccc ggcggcccag gccgcgccga ggacggcgcg aggagcgaga
      601 tgtgtacaat caccagatcg ggagagcttg acttggccga ttcttcccac gacgacgaaa
      661 cgcgacgcct cgacgccgac gagggcgaga cgaggcgcat cgattccgac gagacgcggc
      721 aaatcgatcc gaagggcaac ccaaccgttc actccaagcg cgccgaaacg cggcgcatga
      781 gcggctgggc atccgacccc accggctcgg acgcgacgcg aacggggcac actccatcgc
      841 caccaccccc gccgccagcg tctggcggac gaggggaacg gatcgagccg gcccctcagg
      901 gtggctggcg tgcatcgccg gacgccgcgc cgcctcctcc accggcttac ccgcgttccg
      961 gccggcgcgc cggtggcaaa gctccagcgc cggcgcccgc tgcacgcgtt gcggctcgag
     1021 gcgggcaggt gcggcaaccg gcgttttggc cctcgttcgc gaggaccctg tttatcatgg
     1081 cgatcctggc cggcgtagcg ttcacgaccg tgctcgcagt cggcgccggc ggctacgtct
     1141 acatcgcctc gcagttgccg tcgcccgaag acctctcgca gcgcgcgacc gagctgttca
     1201 ccagcagcca gatttacgac cgggacggca acctgctgtt cgagctgctc gatccgcagg
     1261 gcgggcggcg cacggtcgtc gagctggacc acatgtcgaa atgggtgcgc caagcgacga
     1321 tcgacaccga agacccgcgc ttctaccggc atcctggctt cgatcccatc ggcatcgtgc
     1381 gcgcggtgat ccagaacctg cgcgaaggcg agaccgtatc gggcgcgagc acgatcgccc
     1441 agcagctcgt gcgcaacctg atgctcccgc cggaagagcg cgccgagcgc acggccaacc
     1501 gcaagatcaa agaggccgtg ctcgccgctg aagttacccg ccgttatccg cgcgacaaga
     1561 ttctcgagct gtatctcaac tcggtgtact tcggcaacct ggcctacggc gtcgaagcgg
     1621 cggcgcagac ctacttccag aaggacgcgg aggacctgac gctggccgaa ggctcgttca
     1681 ttgccggcct ggtgcaggcg ccctccgtgt acgatcccta cacgcccgac gggcgcgagg
     1741 ccgcgctcgc ccgccatcgc accgtgctgc gcctgatggt cgaagcaggc agcatcacgc
     1801 gcgagcaggc tggagctgct gccgaggaga tggcagccta cgagttcaag cctccgcgcg
     1861 tcgccgtcga aatcccatcc ccgcacttcg tgcagcacgt gcgccagatc gtcgagactc
     1921 acttcgggcc agaagcgttg tatcggggcg gcctgaagat ctacaccacg ctcgatccag
     1981 agctgcaatc aatcgccgaa gacgtcgttc aggaacagat cgcgggcctg gccgatcgta
     2041 acgtctatgg tggcgcgctg gtggcgatgg agccgaacac cgggcgcgtg ctggcgatgg
     2101 tcggcagccc ggactaccgc gacgagcagc acgccgggca gatcaatatg gccgttgcgc
     2161 cgaggcaaac cgggtcggcg atcaagccgc tcaattacct ggccgccttc gaaaagggat
     2221 ggacgccgtc gacggcaatc tgggacacgc cggtcgtgta caccgacacg gctggcaacg
     2281 tatacaggcc cgtcaactac gacggcaggt tccacggtcc gcagccggtt cgttcggcgc
     2341 tcgcgaattc gtacaacgtt ccggcggtga agaccctcga gtttgtgacc gtgccagagt
     2401 tcctcaaagt ggcgcagcgc ttcggcatca cgacgttcac gcgccccgac tacggcctgt
     2461 cgctcacgct gggcggcggc gaagcgccgt tgatcgaaat ggccggcgcg tttcagatct
     2521 tcgcgaacgg cggcgtgcgc cgcccgccgg tcgtgatcga gcgcatcacc aacgtgcgag
     2581 gcgatgtgca atgccagttc gcgttgcctg ggcaggatgc cggaggcgcg cctccttgcg
     2641 aatcggcgcc cggcgcgggc gagcaggtca tcagcccgca gcatgcgtac ttgatcacgc
     2701 acatcctgtc ggacaatctg gcgcgctgcc ccgctttcgg ctgcccgaac attttggaga
     2761 tcgggcgacc ggcggcggtc aagaccggaa cgaccaacga ctttcgcgat aactggacga
     2821 tcggctacac gccggagctg gttacgggtg tttggatcgg caatccggat cggtccgaga
     2881 tgaagaacat cagcggcgtg accggcgcag gcccgatctg gcacaacttc atgcagcgcg
     2941 cgctagaagg ccaacctgtc ctcgaattcc cgcacccgcc cggtatcatc gagatggaag
     3001 tgtgcgccga ttccggcacg cagccttcgc catactgccc cgccaggcgc accgagatct
     3061 tcgccgccga ccggccgccg ctcggcccgg agcacgactg gtatcagctc gtccggctcg
     3121 acgcggcttc gggcctgcgg tgggttgagg gctgcacgag cggcgtcgtc gagaaggtga
     3181 tggccgtcgt gccgcccgaa ggattcgagt gggcgcagca gcacggactc gaagtcgcgc
     3241 cgatcgaaac gtgcgccgcc ggcccgaccg cgtcgatcgt cgcgatcggc agcccgcccg
     3301 acggcgggtt cgtcgacggc gtcgtgcccg tcatcggcac ggccacgata cccgacttcg
     3361 aaagctacga tctgcaattc gcctcggctg tgtcgcccga cacgtggcgg tggatcagcg
     3421 gcccgcacct ggcgcaggtc gtcgacggcg agctgacgca gtgggatacg aacgggctgg
     3481 aagatggcga atacatcctg cggctggtcg tgcacgcgtt gagcggtgga tcgaccgaag
     3541 cgcgcgtccg tgtgttcatc aatcgcggcg cgatccccac gccgacggat acgcccggcc
     3601 cgacggcgac gccgacgccc gcgccgaccg agacgccggt cgtcgtgccg acgctgcctc
     3661 ccagcaccga gacgcccatg tcggagccga cgctcccgcc ggccgatacg gtcgaaccga
     3721 cgccgacacc gtaaggggag agagtgatca gtcgtcagtg atcagttgtc agtgatcagt
     3781 gtcagtaatc ggttattggt aagtggtgaa tagtaggcgc cgcccatcac gtatcactca
     3841 atacgcctca cgtctcacgc tttacgcttc acgtttcacg cttcacgcac gactctccac
     3901 cactcctctc gtctccgcgc aacttcagtc aatctgccgc agcatcaccg tcgtcgattc
     3961 ccccgtcggc gcaaaaccga aacggcgata cagcgtctgc gaggtgaagt tgttcgcctg
     4021 cgtgttgagc gtgaccgggt acgcaccggc cgcgagcaga tacgtcagct cctcggccaa
     4081 caggcgcgtg ccgatgccgc gcctctgata accggggtgc acggccaggc gaatgagatg
     4141 cccgcgcccg acgtacacgt cgctgaaggc atgaccgacg atgcggccat ccagctcggc
     4201 gacgatgaaa tgcgccgcgt ccccctggat gcgcgcgagc gagtcgagcg tgtgccacca
     4261 gatcgggtcg aaggcgacgc ggtcgttggc gaggacggcg gggaggtcgc caggcgacgc
     4321 ctggcgcagc gtcggggcgg gcgagccttg ctccggaatc gagttgtcgt ccttgcggaa
     4381 gtgcgtcaca tcggtgaagg gcgtgaaacc cagatcgggc aagtaatcgg tcagccagtc
     4441 cgggtaggcc aggcaggcga gtgagcctgt cccggccgcg cgcagcggcg ggagcgcggc
     4501 ggcaaacagc gcctcgagca cggcccgcgc gtcgtatccg tcggcgatag cgatgagccg
     4561 aacccaggca accggcagct cgttcgccgg cgcgatgacc gcgccggcaa tagggttacc
     4621 ggcgacggcc acccacgcgt tgctcgacgg ttcggcgagc cagtcgtcga tcgaccacca
     4681 gtcgaggtgg aaatgcgtgc gccgggactg ctcggtcagc cgagccagcg cgccgcgatc
     4741 ggcctttgtg gccgggcgca cgtcgagtgg ggcgggggcc ggtgggcggc gcgcgaagag
     4801 tcgctgcatg gcgcgactat aacaccccgc catcgtttgc gcaatgccaa accgacttgc
     4861 atcttgcctg cgagtaatgt aaacttacaa tcagtccgga cttcggcaaa ttgccggcgt
     4921 gcagccttta ggctgctata tctgtcgatg ccgcaggctg aagcctgcac tccaatcgcc
     4981 agatatggtc gttgccaaag ttcagaagca tattggaaca gtctgaattc aggagatacg
     5041 ggatgccatc cgacggcaag gctaaggcgc ttgacacggc gctggcgacg atcagcaaga
     5101 aattcggcga cggcgcgatc atgcggctgg gcgaagcgtc ccacctggaa atcgccgcaa
     5161 tcccgaccgg atcgatcgcg ctcgacctgg cgctgggcgt gggcggcgtc ccgcgcgggc
     5221 gcatcaccga gatctacggc cccgaggcct ccggcaagac gacgatttgc cagcacgtcg
     5281 tggccgaggc gcagaggatg ggcgggacgt gcgcgtacat cgacatggag cacgcgctcg
     5341 atccgagcta cgcggcgcgc tgcggcgtgg acgtcgacaa cctgtacgtc tctcagccgg
     5401 acaccggcga gcaggcgctg cagatcgccg acgcgctgat ccattccaat gcgatcgacg
     5461 tagtcgtgat cgactcggtg gccgccctgg tcccgcgcgc cgagatcgaa ggcgagatgg
     5521 gcgacagcca catgggcctg caggcgcgcc tgatgtcgca ggcgctgcgc aagctgagcg
     5581 gcgcgatcaa agccagcaac acggcgatgc tcttcaccaa ccagttgcgc atgaagatcg
     5641 gcgtgatgtt cggcaacccc gaaaccacgt ccggcggcat ggcgctcaag ttctacgcca
     5701 gcgtgcggct ggacgtgcgc cgcatccaga ccatcaaggc cgagggggag gccgccggca
     5761 gccgcgtgcg cgtgcgcgtc accaagaaca aggtcgcgcc gccgtttcgc gaagccgagt
     5821 tcgacatcat gtacaacgaa ggcatcagca aaaccggcga cctgctcgac gtcgggacga
     5881 cgctggacat cgtcaccaag cgcggcgcgt tcttcacgta cggcgatacc aagctgggcc
     5941 aggggcgcga aaaggccaag gagttcctga agcaaaatcc ggagatcacc acgcaaatcg
     6001 acagggacat tcgcacgcac gccgccgagg ccgcgcagtt gacgcggctg gggagcagcg
     6061 ccggcggcgg cgaggacgaa tcggaagaat agattcgggt ttattattcg gcgctttatt
     6121 cttcgccgaa gaaactggat cgccacagct tacttttatt catcgcaggc gagacggcga
     6181 tccgtcgatg atggcagaga tacggccggc ccccggatgg ccgagggcgt gaaggccgga
     6241 tgctacagtc cccgcctcgg gcggggatga cagtcaggcg attcgcagac gcgaattgcc
     6301 ggctcactct ggacagggct gcctgggctt gcctgttgca aggtgaatgg atcggcggac
     6361 ttccggatcg ccgacgctct cgtgcatctc taaccatctc cattgacggg tcgtgtatct
     6421 cctcgcgagc tgtggcgtgt ctgctacggc tcgcgtgttt ttttcagtca gttctctgag
     6481 ttccttgagt tccctgatca gaactgacga ggaacggaag gaactaaagg aacttcagga
     6541 cgtgggcaga gccattaccg cgctcgtcgc gcaaaagaag aacaaggatc gcgtgaacgt
     6601 ctacctggac ggcaagttcg cctttgggct ggcggccatc gaagcggtca agctcaggcg
     6661 cggccaggtc ctgagcgacg aagacatcgc gcggctgcgc gcgctggacg acgccgagaa
     6721 ggcttacgac cgggcgctgc acttcctgag cttccggccg cgcagcgaag ccgaagtgcg
     6781 gcgcaacctc gacgaggccg ggttcgcgcc ggagacggcc gttgcggcga tcggccgctt
     6841 gaaacgcgcc gggctgctcg acgatcgcgc cttcgcgcaa tactggctgg agaaccgggg
     6901 ccagttcagc ccgcgctcgt cgcgcgcgct gaaggccgag ctgcggcgca agggcatcga
     6961 tctgtcgacg atcgacggcg cgctgcaggc ggcagcgcac gacgacgacg aggcggcgta
     7021 ccgggcggcg ctgccacgcg cgcggcggct ggctcacctg gacgcgcggg cattcgcgca
     7081 aaagctcggc gcctacctgg cgcggcgagg cttcggttac gacgtcgcct tggaggcggt
     7141 gaggcgggct tgggaagagg tccgtccgaa cgcgcccgat tcatcattcg aaaccagtga
     7201 ggattaagac atggaactgg gaagcatcgt gctgggagtg attgccggcc tgatcgccgg
     7261 cgctgctctc ggctacactc tctaccgtta tcgacagcag cagcaggggc tgcacgcacg
     7321 cgccgaggcg gagcggctgc tggacgaagc gcaaacgaag gccaaggagc tggagctgaa
     7381 aggccacgac gaagccatcc gcatccgcga tgaggcggag aaggaagcgg cgcggcgccg
     7441 ggccgatctg cagcgcgaag aagagcggct cgcccgcaag cgggaacagc tcgacgaaaa
     7501 gctggaaaaa accgagcagc gtgagcgccg gctcaatcag cgccagagcc agttggacaa
     7561 gcaggcgaac gatctggcca aggtggagca gcagcgcacg gccgagatgg cgcgcatcgc
     7621 gcagatgacc gtcgaggacg cgcgccggga gctgctggtc gccgtcgaga agacggcgcg
     7681 cgacgacatg gcgcgggtga tccgccaggt ggaggcagag atcaaggagg aagccgacag
     7741 gcgcgcccgc gagatcgtca cgctggccat ccagcgcatc gccgccgagg ccggcggcga
     7801 cgtcgccgtc agcgtcgtgc ccctgcccaa cgacgagatg aagggacgca tcatcggccg
     7861 cagcgggcgc aatatccgcg ccatcgagaa ggcgaccggc gtggacatcg tcgtcgacga
     7921 tacgccggag gcggtgacgc tgtcctcatt cgacccggtg cggcgcgagg tggcgcgcgt
     7981 cacgctgaca aacttgatcc aggatgggcg cattcacccc gcgcgcatcg agaagctcgt
     8041 ggaagacgcg cgcgccgaag tggaccgcgt catcaaggag gaaggcgagc gcgccactat
     8101 cgaggccggc gtcccgggcg tgcatcccga agttcagaaa atgctcggcc gcctgaaatt
     8161 ccgcacgtcg tacggccaga accagttgta ccacgcgatc gagacggcgc ggctggccgg
     8221 gatcatggcc gccgagctag gcgcagacgt caaggtctgc aaagcaggcg gcctgctgca
     8281 cgacctgggc aaggcgatgg atcacgaagt ggaaggctct cacgccctga tcggcgcgga
     8341 cttcgccagg cggcacgggg tcgacctgcg cgtcgtaaac tgtatggcca gccaccatca
     8401 cgaagtggag gcagaatcgc tcgaagccgt catcgtcgag gctgccgatg ccatctccgg
     8461 ctcgcgcccg ggggcgcggc gcgaatcgat cgagctctac ctcaagcgcg tgaaagacct
     8521 ggaagatatc gccagctcct tcgagggtgt cgcgcaatcg tatgccatcc aggccgggcg
     8581 cgagatccgg atcatcgtgc ggcccgagga catcgacgat ctggcgtcgc ttcagctcag
     8641 caagaacatc gcccggaaga ttgaagagac catgcaatac cccggccaga tcaaagtgac
     8701 cgtgatccgc gagacacggg cggtggacta cgctaagtga acattggcag tagaaaccgg
     8761 tcacatcccc agcccgccca acgaaaggcg ggcgttttca tcccacggtg ggaacctctg
     8821 tgcgtcgagc ggtgtatcca tagaggatag catccaaaat gccacattgc acagaggagg
     8881 gatcggaatg agagccatta ccgttctatg cctgttcctt atcttaacgg catgcaccgg
     8941 gcaggggcag gtgacagggg agcctggcgc gccaaccgag atgccggcaa ggcttccagt
     9001 gcagccgacg gctacgtcgg agccggccac ggagacgccc gcaaagcctg cggcgcaacc
     9061 agcggcgatc ccctctttcg aatcgagtgt gctcctggct cagtggaacg cgaacgagcg
     9121 ccgccacgaa atccttccgg ttgatccggc cacaggccag gcagtgcccg gccaggcgcc
     9181 gctcatcatt ggcaagattt cccagtttgc cggagtgcaa accctctcgt cggacggcag
     9241 atggctggca gcgatcgagt ctcatggcca ggtctctgaa cccaatggtg gaggaaccag
     9301 tcactattcc agcgctgatg tgctgcatct ggttgacgtg acagcctggc gggcggtgac
     9361 gaccacgctc ccaggaaggg gttgggtttg gccaatcact ttcagccctg ataccgcacg
     9421 cctggcactg ggctatcaca accgaacctc cagcacactc atgctcttcg acacgggcac
     9481 agggcagctc atcgctcaac aagcactggc attccgtccc tcgttgatga agtacacgca
     9541 agagggaacg gcgctggcaa tctatggcca gccgctctct gccgtgccag ggatagcgaa
     9601 acccgacccg ccgcgcgtgc tgctggtcga tgccgcgacc ctcgacatcc tgtgggatca
     9661 accgctggca agcgtcatca gcggctcgtg gtgcctggaa aactgcgacg cgccgcatgg
     9721 ggagcagatg ttcgccggct ggtggccggc ggtcgtcccc tcccacgatg ggcacacgct
     9781 ctacatcgtt cacgccgacg aggagaagct gacggcggtg gacttcgacg ctcgcacggt
     9841 gcacagtgtt gagatccggg aagcgcggtc gtggtttgaa gagctgttgg ccctcacggc
     9901 cggcgtcgcc gaggcgaagg gtggcgagaa tggggccttc aaagaggcag tgttgtcacc
     9961 cgatggaacg cggctctacg tggtcggccg cacggtgaat tctacacgcg atgcgcgcgg
    10021 cgagtggcaa gtcacggagg ccttcctcgg ccttcaggtc attgatgtcg agagcggtcg
    10081 caaggtgacc ggcctcgacg gccaggcgaa ggccatcggg acaacgccgg atggggccta
    10141 cctgttgctg gatgactggg acgggcgcgt ctggtggacg gaggtgctcg atgccgagag
    10201 cctgcagcgc gtggcgcgcc tggagagatg ggcggttgta ccggctcggc gaattgacgg
    10261 ccagcccatc ctcttggcga gcgaccccta ccgggggtca cagtttgcgg tgctcgaccc
    10321 tcgatcgttc gacattgtcg acacctggtc ggtgagcgaa cacgcagagt gggtaacaac
    10381 gagtccttga acgaggtcgc agcccttttt caggtcacgt acctgcgagc caaccattac
    10441 accagccaac tacgctacgg caggtagaac cgggggtttc gccagaaacc tggtttctcg
    10501 aagaaaccag gtttctccat gagcgcctac ggcaagtaga atctcgggtt gatgagaccc
    10561 tcgacggtgc gaatctcgaa gtgcaaatgc ggcccactgg agtggcccgt cgacccgacg
    10621 gtaccgatca ggtcgccgcg ccggatggcc tgcccgagca cgacgttgaa gccgctgagg
    10681 tgcgcgtacc acgaggcgaa gccgttgccg tgatcgatgc gaatcagata gccatagccg
    10741 atatcggtcc agccgacgaa cgacacgaag ccgccgtctg ctgcgtagac cggcgtgccc
    10801 gtcggcgcgg cgatgtcaac cgctcgatgg ccgtaccagt aatcctgcgt gattcggccg
    10861 acgaccggcc actgaaagcg cccggtgccg cgcgcgccgg taggaatcgg gccgttgtag
    10921 gcagtgacga gcttggggac gtacggcttg ctgccgccgg cgacgatcag cttcatgccg
    10981 ggcgtaatcg tgtaatcggg cgccggcaac tgattccact cgttagccgc gccggtgatc
    11041 gcgtcgacgt cgaccttgta cttggcggcg atcgattgga gcgagtcgcc ctctttcacc
    11101 tcgtgataca cgccgtcgat aggcaggacg acgatttcct ggccgatgcg cagcagatcg
    11161 ggcaagtcct cgaccgccgg attggcccac atgatggttt cgggattgag accataggct
    11221 gcggcgatcc cctgcacggt gtcacctggc tgcacgacgt agacgacgat gtcgcgacgt
    11281 gtccggtaag ggatactggt atgcaaatcg gcgaggcgcg acacggaatt gccattgctg
    11341 gcgaagaatc cgcgcgtggc gaacagctcg aaggacggct gaggcaaggg ttgggcgatc
    11401 gacgcggaca ggatgccggc ggctactgag tcgacgcggg gtgcggcgct catgccgacg
    11461 gcgatcacgg ccagcatcag catcgtgata tgcaccgcgt atcggcccag gcgcgccccg
    11521 g
//
LOCUS       DBNHXB010000482         6680 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3514170, whole genome shotgun sequence.
ACCESSION   DBNHXB010000482 DBNHXB010000000
VERSION     DBNHXB010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3514170"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..2839
                     /locus_tag="AC162G_18575"
     CDS             191..2839
                     /locus_tag="AC162G_18575"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5077866.1"
                     /translation="MPGLLTPDVRICLLGRIEVARGDRVLRANDWSRRKAQTLLARLA
                     IEPSRRLLKDQALDLLWPDQTVAAASNNLYRTLHSLRQTLDTHLGPGSANSMITYEDG
                     VFTLGDSVQIDAAEFERLCSTPATSESRIPNLQSALSLYRGDLLPDDLYSDWTLSPRE
                     SLRRLHREASITLAHHRIQSRDYPAAIVLLLPLIDRDRADEPVHRSLMRAYALAGRRH
                     DALRQYQACVDALAADLDLPPEPETTALYDQILSGELSPAPEPVTATRNALPAIVLKI
                     AHSDHLIGRDTEFETLIGWLRAAGQGQGSTILVGGDSGVGKTLLAAETLCASAGSGMT
                     PLFGAAYEQEGQLAYQPFVEAFDRYLADSGGLPDENPITHFKRTGSDPQQEQWALFNA
                     VAMFLTDLAKGSPVVLLIDDLHAADEASLRLFHYLARQTRKTPVALLATYRADLAVAA
                     VPFGTLLNALYRERLSETLALDRLPKEDVARLLAHTLGGDATPALVDAVYEIGEGNPF
                     FIQELSRALIDEEQIERRDSQWHLIEGKELRVPTGLSGLLRERVHRLGPSVGAALTTA
                     AVIGREFGFDVLRATAELPDSVLLDALDTALAGQLLDETESGYRFRHILIRRVLYDSL
                     SRARRAHLHGHAAEAIESVYARRPGRLAPHIEDLAHHYDRSDRRERALNYLIEAGRKA
                     ARVFAFEVAADYYERALALLDALGLPEAAQRFHLLEQLGKYYKVLADTPKAVAAFERA
                     LDVSGEGWKPRACDRARTRRLAAMGLLTAGRLDEAETQLQRALAELSGEGEEDEIELA
                     NVLYNVAQLHWHRNEYRAAFDVAQRSLAVAERVNDREAIARAFEMLALACHSLGEWQT
                     GMDYEQKRAALVGPGLDVGDAFDVHL"
     gene            2882..3673
                     /locus_tag="AC162G_18580"
     CDS             2882..3673
                     /locus_tag="AC162G_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5077867.1"
                     /translation="MKQTVAATQEQARRMNAPRAIALCQCFNGTLEFQAGHWAEADVA
                     LRESIKLHRELGAASGEALAWQRLGVLQTARRQLDEAMASFDEGVVVAERAVMRAHCL
                     ARLYASMTRNRLIAGDVQAADQYLALGLAMGQRHGNCATCDSLLFPAAVGVRAAQGDF
                     AAAEAFCGKMDEAAERYASRVWIAMARQSRGELTAAEGKIDEALAHYAEAHAAYSAAG
                     NEYEAARCLESTAHLHQKRRAAGDAQMAQSAQAEARQIFERLGAM"
     gene            3820..4059
                     /locus_tag="AC162G_18585"
     CDS             3820..4059
                     /locus_tag="AC162G_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077868.1"
                     /translation="MGTRCTFYICTICFEVSGTPSEHHSRQMIRCDAGQPGDEARRPL
                     VDAEGHLKTHAPRWFLQAIGSLPARPYAQSLISNL"
     gene            4083..4865
                     /locus_tag="AC162G_18590"
     CDS             4083..4865
                     /locus_tag="AC162G_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_391899447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR5077869.1"
                     /translation="MSNAIIEGLNLNIDELRASIREEYTAVASDPGRGFHFHTGRRLA
                     KTLGYKDEWIDALPHGAVESMAGTGNPFSLGELQSGEYVVDAGSGAGADSLIAARMVG
                     PAGRVIGVDMTPEMLAKARRNAEEAGLTNIEFREGYLESLPVPDGWADVVISNGVLNL
                     VPGKAQALAEMYRVLKPGGRLQIADIALQKPVPGDAVSDVSLWTGCIAGGLVKAILEK
                     KVKDAGFKDVEVIWGADVFSGAPQHSDAAEFGTLGVHIHARR"
     gene            5231..6217
                     /locus_tag="AC162G_18595"
     CDS             5231..6217
                     /locus_tag="AC162G_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077870.1"
                     /translation="MDFRHRTFQWGRAARRVIPAVFAFALGLSSGYALWGRATLASGV
                     AVPSTPKAAVVDATPAPAPANRIDLAALSQQVNPPGGYTLPAKFGDIGPKLLAAGGID
                     FDRFVQLYEQGGQPLTDEQIAILKESSAASIVIDRENASFLLNVFWAVGLTNRNRILT
                     EGPMMQGGKDQVGNFASTAGWTMGARPPAELYASASLITLTSEQQLRLEEVAQNVYRP
                     CCNNPTHFPDCNHGMAMLGLLELMASQGASSDEMFEAAKFINAFWYPQQTLEQAIYFE
                     AAEGLDFSQVEPRTIVGVNYSSASGYRAVRQWLASSGLLKQSSGGGSCSVSP"
     gene            complement(6341..>6680)
                     /locus_tag="AC162G_18600"
     CDS             complement(6341..>6680)
                     /locus_tag="AC162G_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077871.1"
                     /translation="GLILNSAVGGVGLGLSTGQLVVGSVAHSEAKDTATAAGLFGLSR
                     QLGGVVGASLTPMFLSFAAPLMAVSSTLFQRGHGDYALTFVFLALIQGLGLAVYVFAH
                     IGAISRRSDF"
BASE COUNT         1245 a         2065 c         2130 g         1240 t
ORIGIN      
        1 ccgtcttttc ggcattggct ctgatcggtt cttccaggtg cggatggaca ccacgtgagt
       61 ggtcgggata cagccctgct tcaccgtttc tgctacaatg tatcggccag gagtgggtag
      121 ccgcagcttg tttgtctccg gctcttggct gaagttcacg tgttagtcct tgcagactgc
      181 gagggtaagt atgccgggct tactgacgcc cgatgttcgc atttgcttgc tcgggcgaat
      241 tgaagtggcg cggggcgatc gagtcctacg cgcgaacgat tggtctcgtc gcaaagcgca
      301 gacgctcttg gcgcgtttgg cgatcgaacc ctcgcgtcgc ctgctcaaag atcaggctct
      361 cgatctgctg tggcctgacc agaccgtcgc cgctgccagc aacaacctct accgcacgct
      421 ccattcattg cgccagacgc tcgatactca cctcggcccc ggttccgcca attcgatgat
      481 cacctacgaa gacggtgtat tcacgttggg cgactcggtg cagatagacg ccgccgagtt
      541 cgagcgattg tgttcgaccc ccgcgacctc tgaatctcga attcctaatc tccaatctgc
      601 cctatccctc tatcggggtg atcttctgcc cgacgatctc tactccgatt ggacactctc
      661 cccgcgcgaa tcgctgcgcc ggctgcatcg cgaagcgagc atcacgctgg cgcatcaccg
      721 tattcaatcg cgcgactacc ccgccgcgat cgtgctgctc ctgccgctca tcgatcgcga
      781 ccgcgccgac gagccggtgc atcgatcgct gatgcgcgcc tacgcgctgg cgggtcgccg
      841 tcacgatgcg cttcgacagt atcaggcctg cgtcgatgcg ctggccgccg atctcgacct
      901 tccgcctgag ccggagacga cggcactgta cgatcagatc ctgagcggcg aactgtcccc
      961 agcgcccgaa ccggtcacgg cgacccgaaa cgcgcttccg gccattgtgt tgaaaatcgc
     1021 gcacagcgat catcttattg gccgcgacac cgaattcgag acactgatcg gctggttgcg
     1081 cgcggcagga caagggcagg gcagtacgat actggtcggg ggcgattcgg gcgtgggcaa
     1141 gacattgctc gccgccgaga cgctgtgcgc ctcagccgga tcgggcatga cgccactgtt
     1201 cggcgcggct tacgagcagg agggccagct cgcctaccag cctttcgtgg aggcgttcga
     1261 tcgctatctg gccgacagcg gcggcctgcc cgacgagaac cccatcacgc acttcaagag
     1321 aaccgggtct gatccgcagc aggaacagtg ggcgttgttc aacgccgtcg ccatgttcct
     1381 gaccgatctc gctaaaggat cgccggtcgt tctgctcatc gacgatctgc atgccgccga
     1441 tgaagccagc ctgcgcctgt ttcactacct ggcgcggcaa acgcgcaaga cgccggtggc
     1501 actgctggcg acctatcgcg ccgacctcgc cgtggctgcc gtgcccttcg gcacgctgct
     1561 caatgcactg tatcgcgaac gattgagtga gacgcttgcc ctggatcgat tgcccaaaga
     1621 agacgtggcc cgcctgctgg cacacacgct gggcggtgac gcgaccccgg cgctcgtcga
     1681 tgccgtttac gagatcggcg aaggaaaccc cttcttcatc caggagctct ctcgcgcgtt
     1741 aatcgacgag gagcagatcg agcgacgaga tagccagtgg catctgattg aaggaaaaga
     1801 gctgcgggtt cccactggat tgagcggcct gctgcgcgag cgcgtccacc ggctcggccc
     1861 gagcgttggg gcggcattga cgacggctgc cgtgattggc cgcgagttcg gattcgacgt
     1921 gttgcgcgcg acagccgagc tgcccgacag tgtattgctc gatgcgctcg acacggcgct
     1981 cgccggtcaa ctgctcgacg aaaccgagag cggctatcgc tttcggcaca tcctcatccg
     2041 gcgcgtgctg tacgactcac tcagccgggc acggcgggcg catctgcacg gccatgcggc
     2101 cgaagcgatc gagtcggtgt acgcgcgacg ccctggccgg ctcgctccgc acatcgaaga
     2161 cctggcgcat cactacgatc ggagcgatcg gcgtgagcgc gcgctgaact atctcatcga
     2221 agccggtcgc aaggcggcgc gcgtgttcgc gttcgaggtc gccgccgact actacgagcg
     2281 cgcgctggca ctgctcgacg cgctgggcct gcccgaagcg gcgcaacgct tccacctgct
     2341 tgaacaactg ggcaagtatt acaaggtgct ggccgacacg ccgaaggccg ttgccgcgtt
     2401 tgagcgcgcg ttggacgtgt cgggcgaggg ctggaagccg cgcgcgtgcg atcgggcgcg
     2461 cacccgccgt ctggccgcaa tgggcttgct cactgccgga cgcctcgatg aggcagagac
     2521 tcagttgcag agagcattgg ccgagctcag cggcgagggc gaagaagatg aaatcgagtt
     2581 ggcgaacgtg ttgtacaacg tcgcgcagtt acactggcac cgcaacgagt accgggccgc
     2641 cttcgacgtc gctcaacgca gtctggccgt cgccgagcgc gtaaacgatc gcgaagcaat
     2701 cgcccgcgcc ttcgagatgc tggcactggc gtgccattcg ctcggcgagt ggcagaccgg
     2761 catggattac gagcagaagc gcgcggcgct ggtcggccct ggcctcgacg tgggcgacgc
     2821 gttcgacgtg catctttgac tgtgggagta tcacctgtat ggtgataaag gctacgatga
     2881 agtgaagcag actgtcgccg cgacgcagga gcaggcgcga cggatgaatg cgccgcgcgc
     2941 aatcgcatta tgccagtgct tcaacggtac gctcgaattt caggccggtc attgggccga
     3001 ggccgatgtc gcgttgcgcg agtcgatcaa gttgcaccgc gaactcggcg cggcatctgg
     3061 cgaggcgctg gcgtggcagc gattgggcgt cttgcagacg gcgcggcgcc agctcgacga
     3121 ggcgatggcg tcgttcgacg agggtgtcgt cgtcgcggaa cgtgccgtca tgcgcgcgca
     3181 ctgcctggcg cggctgtacg catcgatgac gcgcaatcga ttgatagcgg gcgatgttca
     3241 agcagccgat caatatctcg cgctcgggct ggcgatgggt cagcgtcacg gcaactgcgc
     3301 gacgtgtgac tcgttgctat tcccagccgc agtcggcgtg cgcgcggcgc agggcgactt
     3361 tgcggctgcc gaggctttct gtggcaagat ggacgaagcg gcggagagat atgccagccg
     3421 ggtgtggatc gcgatggcgc gccagtcgcg cggcgagctt acggcggcag aaggcaagat
     3481 cgatgaagcg ctggcgcact acgccgaagc gcacgccgca tattccgcgg ctgggaacga
     3541 atacgaagcg gcacgctgcc ttgaatcgac ggctcaccta catcagaagc gtcgcgctgc
     3601 gggtgatgcc caaatggcgc aatccgcaca ggccgaggcc cggcagattt tcgagcggct
     3661 gggcgcgatg tgaggatttt attcgacgcc aagacgcacc gacgcgaaaa aagcgcctcc
     3721 cgaaggcatc catcttcggg aggttttgtt tcgtcaagag gtaagaaatt tgggaggtcg
     3781 gtcgtatatt ctgggcgcag tttcaaggga gatgagccga tgggcacccg atgcacgttc
     3841 tatatctgca cgatttgttt cgaggtcagc ggcacgccaa gcgaacatca tagccgccag
     3901 atgatccggt gtgacgccgg gcagcccggc gacgaggcgc gcaggccgct cgtcgacgct
     3961 gagggccatc tgaagactca cgcgccgcgc tggttcctcc aggccatcgg ctcgcttccg
     4021 gctcgcccgt atgcccaatc tctgatctcg aatctctgat cttcaaggag gagacatcca
     4081 ccatgagcaa cgcaataatt gaaggcctga atttgaacat cgacgagctg cgcgcgtcca
     4141 ttcgcgagga atacacggcg gtggcgagcg acccggggcg cgggtttcac ttccataccg
     4201 gccgcaggct ggcaaagacc ctcggctaca aagatgaatg gatcgacgcg ttgccgcacg
     4261 gcgcggtcga atcgatggct ggaaccggca acccgttcag tctcggagaa ctccagtcgg
     4321 gcgagtacgt cgttgatgcc ggatcgggcg cgggtgcgga cagcctgatc gccgcgcgga
     4381 tggttgggcc ggccgggcgc gtgattggcg tggacatgac gcctgagatg ctagccaagg
     4441 ctcgacgcaa cgcggaagaa gccgggctga cgaatattga gttccgcgag ggttatcttg
     4501 agtcgctgcc ggtgccagac ggatgggctg atgttgtgat ctccaatggc gtgctcaacc
     4561 tcgtgccggg taaggctcag gcgctggcgg agatgtatcg cgtgcttaag cctggcggtc
     4621 gattgcagat cgccgacatc gcgttgcaga agccggtgcc cggcgatgcg gtcagcgatg
     4681 tgagtttgtg gacggggtgc atcgcgggcg ggttggtcaa agcgatcctg gaaaagaagg
     4741 tgaaggacgc cgggttcaag gatgtcgagg tgatctgggg tgcggacgtt ttcagcggag
     4801 cgccgcagca ctccgatgcg gccgagtttg gtacgttggg tgtccacatc cacgcgcgca
     4861 ggtgaacagg cgccagttct cccacccaac ccacacccgc tgtctaccta atcttcacct
     4921 tcacctgata cactcacgcg gcaggaggca ccatagccga gacatcctac cgcgtcgcca
     4981 tacccgacct gaccttctac cagcagacag tcgcctgcca gtttgcctgt ccggtgcgta
     5041 ctgatgggcg ggcttacggc gcggaccgac gactttcccc cggcctggta tcgcagcacc
     5101 atttccttac ggtcgcctgg ccgaaggcat gcccacacgc accgcacgac tgcatccccg
     5161 ccatttggcc tactcgaaaa ctggcgaaat ggcgctggtg aggccactag ttgccgcgta
     5221 ccatgagggc atggacttcc gtcaccgaac ctttcagtgg gggcgagcgg cccgacgggt
     5281 cattccggcg gtcttcgctt ttgccctcgg gttgagcagt ggctacgcct tgtggggacg
     5341 cgccacactc gcgtccggag tggcagtgcc gtcgacgccg aaggcagccg tagtggatgc
     5401 cacgcccgcg ccggcgcccg ccaaccgtat cgacctcgcg gcgctgagcc agcaagtcaa
     5461 tccaccagga ggctacacct tgcccgccaa attcggcgac atcgggccga agttgctggc
     5521 cgccggcggg atcgacttcg accgcttcgt gcaactctac gagcaaggcg ggcagcccct
     5581 gaccgacgag caaatcgcca ttctgaaaga gagcagcgct gcgtcgatcg tgatcgatcg
     5641 cgagaacgct tccttcctgc tcaatgtctt ctgggctgtg gggctgacga accgcaaccg
     5701 catcctgacc gaagggccga tgatgcaggg tggcaaagat caggtcggca atttcgcttc
     5761 gaccgcgggc tggacgatgg gagccaggcc gccggccgaa ttgtacgcga gcgcatcgct
     5821 catcacgctg acgtccgagc agcagttgcg cctcgaagaa gtcgcccaga acgtgtaccg
     5881 gccctgctgc aacaacccga cacacttccc tgattgcaat cacggcatgg cgatgctggg
     5941 actcctggaa ctgatggcgt cgcagggcgc gtcgagtgac gagatgttcg aggcggccaa
     6001 gttcatcaat gcgttctggt atccgcagca gacactggag caggcgattt acttcgaggc
     6061 cgccgagggg ctggacttct cacaggtcga gccgcgcacg atcgtcggcg tcaattactc
     6121 gtccgcgtcc ggctaccgtg cggtgcgcca gtggctggcg agcagcggct tgctgaagca
     6181 gagttccggc ggcggtagtt gcagcgtctc tccataagcg ccagcccttt gtatttattg
     6241 gtttatgctc aaacacgcca gttggcgctt ctgggaactt ctttcctcgc actgttgtag
     6301 gtggatgtcc atcacaacct ccctgtttgg ggatgccgga ctagaaatca ctgcgccgac
     6361 tgattgcccc gatgtgagca aagacataca ccgccaagcc aagcccctga atcagggcta
     6421 agaagacgaa ggtcagagca taatctccat gcccacgctg gaagagcgtg gagcttacag
     6481 ccatgagagg cgctgcgaac gagaggaaca tcggcgtcag tgacgccccc actacaccgc
     6541 ctaactgcct cgacaaacca aacagccctg cggctgtggc ggtatctttg gcctcactgt
     6601 gggcaacact ccccactacc agttgacctg tggacaatcc cagacccact cctcccacag
     6661 cggagttgag gatgagacct
//
LOCUS       DBNHXB010000483         3799 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4016861, whole genome shotgun sequence.
ACCESSION   DBNHXB010000483 DBNHXB010000000
VERSION     DBNHXB010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4016861"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..929)
                     /locus_tag="AC162G_18605"
     CDS             complement(6..929)
                     /locus_tag="AC162G_18605"
                     /inference="COORDINATES: protein motif:HMM:NF015509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR5077872.1"
                     /translation="MNELLGILAHDILPIFVVMGLGFAFDRRFKPDVHSLTRITFYIF
                     SPSLVFSSLVKSELASAEMLQIAAFTVGVSLIMGLLGLAASRLLQLDARATAGLVLVC
                     MFVNAGNYGLGVNLRAFNEEALARAVVYFTTSTLLVYTLGVWVAAGSDGSGWRTTLRR
                     TLAVPPMYAAVAAILVRALAIDMTQPALEPLLAGIDLAGRAAIPLMLAILGMQLSRTS
                     VTAHLRPSAAATGLRLVVAPVIAWGAAAVIGLNGVARQASILEASMPAAVINIILATE
                     YHAAPALVTNAVIVSTLCSPITLTLLIALLK"
     gene            complement(926..1891)
                     /locus_tag="AC162G_18610"
     CDS             complement(926..1891)
                     /locus_tag="AC162G_18610"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR5077873.1"
                     /translation="MDALDSLLKNMTNEEEMLSVAKLYALSGLDRDELERVRRVWPST
                     AVDRRQAIMRHLVEISEANFEVDFGSIFRIGLADGDPEVRATAIDGLWEEEDVKLVPT
                     LLDLLRNDSSETVRAAAATSLGHFVLSGELDEIPTAKLEQVVDSLRDVFEDDSETLDV
                     RRRAVEAIGYSSADGVADLIAEAYADDEEPMRISAMFAMGRSADLRWADPVLAEVDSP
                     NPEMRYEAARACGELQNLDAIPTLTTLLDDPDDHVREATVWALGQIGGNEARRLLTGI
                     LEDEESDLHEVAEAALDELEFMSGSDLDFPMFDFNPDDDETDRDA"
     gene            complement(2048..3367)
                     /locus_tag="AC162G_18615"
     CDS             complement(2048..3367)
                     /locus_tag="AC162G_18615"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HIR5077874.1"
                     /translation="MSDLATQVIAFLIVLGPLVMLHELGHFVFAKRAGIRVQEFGLGF
                     PPRARKLWRGMGRLRIGAESVHSPRNFKFPSGLEEGAVVEARADEVKGRLMLRSIKVV
                     DAKQEGAVTTPLKVADADGVRLRGELAYFDPGTDYTLNWLPIGGFVRMLGEEDPSKPD
                     SFAAALKRWRAAVLLAGPGMNLLAALVIFTMAYLLGQPVVDRIEVVIGAVAAGAPAEQ
                     AGLVPGMAILAVDGIAVDAAETLTAYTHAHLGETIELTVRDVDGTQRVIDVHARPQPP
                     QGEGPMGITIGQNPLSYKIVSNSLPEAASRALESTGDVLNGMITLPRLLLAGEIDPGL
                     ARPVGPAGIAQISGYALAESIERQVWFPILNMAAAISVALAFTNLLPLPALDGGRLVF
                     VLIEAVRGKRIAPEKEAVVHFAGMMFLLALAVLITIQDVSNPIPNPF"
     gene            complement(3476..>3799)
                     /locus_tag="AC162G_18620"
     CDS             complement(3476..>3799)
                     /locus_tag="AC162G_18620"
                     /inference="COORDINATES: protein motif:HMM:NF037336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HIR5077875.1"
                     /translation="ILSRDFASLAPVVEADALAMHAVMMTSNPPLLYWQPPTVAILHA
                     VRAWRADGLPVCFTIDAGANVHCLCQSSAADEVARRLRSIAGVQNVLAVSPGGPAHLV
                     ESHLF"
BASE COUNT          692 a         1274 c         1229 g          604 t
ORIGIN      
        1 cgtcgtcatt tgagcagcgc gatgagcagt gtgagcgtga tcgggctgca cagcgtcgag
       61 acgatcacgg cattcgtgac aagagcggga gcggcgtgat attccgtcgc caggatgatg
      121 ttgatcacgg cggccggcat gctggcttcg agaatggacg cctggcgcgc cacgccgttc
      181 agtccgatca cggccgccgc gccccaggcg atgaccggcg cgacgaccag gcgcagcccg
      241 gtggcggcgg ccgacgggcg caagtgcgcc gtcaccgacg ttcgagacaa ctgcatgccg
      301 aggatcgcca gcatgagcgg gatggcggcg cgcccggcga ggtcgatccc ggcgagcagc
      361 ggctcgagcg ccggctgcgt catgtcgatg gccagcgcgc gaacgagaat tgcggccacc
      421 gccgcataca tcggcggcac ggccagcgtg cgtctcagcg tggtgcgcca gccggagccg
      481 tcgctgccgg cggcgaccca cacgccgagc gtgtacacca ggagcgtgct ggtcgtgaaa
      541 tacacgacgg cgcgcgccag ggcctcctcg ttgaaagcgc gcaggttcac gcccagaccg
      601 tagttgccgg cgttcacgaa catgcacacc agcacaagcc cggccgtggc gcgcgcgtcg
      661 agctgcagca ggcgactggc cgccagaccg agcagcccca tgatcagcga gacgccgacg
      721 gtgaacgcgg cgatctgtaa catctctgcg ctggccagct cggacttcac gagcgagctg
      781 aataccaggc tggggctgaa gatatagaag gtgatgcgcg tcaggctatg gacgtcgggc
      841 ttgaagcgcc ggtcgaatgc gaatcccagc cccatgacga cgaagatagg cagaatgtcg
      901 tgggccagga tgccgagcag ctcattcaag cgtcacgatc cgtttcgtcg tcgtccgggt
      961 tgaaatcgaa catcgggaag tccaggtcag agccgctcat gaattcaagc tcgtccagcg
     1021 ccgcttcggc gacttcgtgc aggtcggact cctcgtcttc caggatgccg gtcagcaagc
     1081 gccgcgcctc gttgccgccg atctggccga gcgcccagac ggtcgcctcg cgaacatggt
     1141 cgtcgggatc gtcaagcaaa gtcgtgagcg tcgggatcgc gtcgagattc tgcaattcac
     1201 cgcacgcgcg cgccgcctcg tagcgcatct ccggattggg cgagtccacc tcggccagca
     1261 ccgggtcggc ccagcgcaag tcggccgagc ggcccatggc gaacatggca ctgatgcgca
     1321 tcggctcttc gtcgtccgcg tacgcttcgg cgatgaggtc ggccacgccg tccgcgctcg
     1381 agtagccgat cgcctcgacc gcgcgccggc gcacgtcgag cgtctcgctg tcgtcctcga
     1441 acacgtcgcg cagcgaatcg accacctgct ccagcttcgc cgtcggaatc tcgtccagct
     1501 ctccggagag cacgaaatgc ccgagtgacg tcgccgccgc tgcgcgcacc gtctcagacg
     1561 aatcgttgcg cagcaggtcg agaagcgtcg ggaccagctt gacatcctct tcttcccaca
     1621 agccgtcgat ggcggtggcg cgcacttccg gatcgccgtc ggccaatcct atgcggaaaa
     1681 tagacccgaa gtcgacctcg aaattggctt cggatatctc gaccaggtgg cgcatgatcg
     1741 cctggcggcg atcgacggcg gtcgacggcc acacgcggcg cacgcgctca agctcatccc
     1801 gatccaggcc cgacagggcg tacagcttcg caaccgacag catctcctct tcgttggtca
     1861 tattcttcaa gagggaatcg agtgcatcca tgtatctatt tccttgagag tattgtacct
     1921 gaacttcgtc actacgcaca acaggcggga catccgtccc gcccgtgtaa tcgatcgctg
     1981 ccgtcaacgt gcgccgcact tccaaccctc tcccttcgct gcgagggcga gagtgcgtcg
     2041 tacgtcacta gaacggattc gggatagggt tgctcacatc ctgaatggtg atcagcacgg
     2101 cgagcgccag caggaacatc atgcccgcga agtggacgac ggcttccttc tccggcgcga
     2161 tgcgcttgcc gcgcacggct tcgatcagca cgaacaccag ccggccgccg tcgagcgcgg
     2221 gcagcggcag aaggttcgtg aacgccagcg cgacgctgat cgcggccgcc atgttcaaga
     2281 tcgggaacca cacctggcgc tcgatgctct ctgcgagcgc atacccgctg atctgcgcga
     2341 tacccgccgg cccgaccggg cgggccagcc cgggatcgat ttcgccggcc agcagcagcc
     2401 tcggcagcgt gatcatgcca ttgaggacgt cgcccgtcga ctccagcgcc ctggacgccg
     2461 cttccgggag cgagttggac acgatcttgt acgacagcgg gttctggccg atggtgatgc
     2521 ccatcggacc ttcaccctgc ggcggctgtg gacgggcgtg cacgtcgatc acgcgctgtg
     2581 tgccgtccac atcgcgcacc gtcagctcga tcgtctcgcc gagatgagcg tgcgtgtaag
     2641 ccgtcagcgt ctccgccgcg tcgaccgcga tcccatctac ggcgaggatg gccatgccgg
     2701 gcacgagacc ggcctgctcg gcgggcgcgc cagccgccac agcgccgatg acgacctcga
     2761 tccgatccac aaccggctgc cccagcaggt aagccatcgt gaagatgacc agcgcggcaa
     2821 gcaagttcat tcccggcccg gccagcagca cagccgcgcg ccagcgcttg agggcagcgg
     2881 cgaagctgtc gggcttcgac ggatcttctt cgcccagcat gcgcacgaaa ccgccgatcg
     2941 gcagccagtt cagcgtgtag tcggtgcctg ggtcgaaata ggccagctcg ccgcgcaggc
     3001 ggacgccgtc ggcgtcggcg accttcagcg gcgtcgtcac cgcgccctcc tgcttggcgt
     3061 cgacgacctt gatcgagcgc agcatcaggc gacccttgac ttcgtcggcg cgcgcctcga
     3121 cgaccgcgcc ctcttccagg ccgctcggaa acttgaagtt gcgcggcgag tggaccgact
     3181 ccgcaccgat gcgcagccgg cccatgccgc gccacagctt gcgcgcgcgc ggcgggaagc
     3241 ccagcccgaa ctcctgcacg cgaatgccgg cgcgcttggc gaaaacgaaa tgcccaagct
     3301 cgtgcagcat gaccaatggc ccgagcacga tcaaaaatgc tatcacctgc gtcgctaagt
     3361 cgctcatgtg ccctcgcttc gtatgccccg ataccgtgaa gattataccc cccgcatatt
     3421 agaatgacaa tagtacggcg taggggcgac aacagtaggg ttttgcagat ggaatctaga
     3481 acaggtgact ttcgacgagg tgcgccggac cgccggggga gacagccaag acgttctgga
     3541 cgccggcgat ggatcgaagc cggcgagcca cctcgtccgc tgccgaggat tggcacaagc
     3601 agtgcacgtt cgccccggcg tcgatcgtga agcacaccgg cagcccgtcc gcgcgccagg
     3661 cgcgcacggc gtggaggatc gccacggtgg gcggctgcca gtagagcagg gggggattcg
     3721 aggtcatcat caccgcgtgc atggcgagcg cgtccgcttc gacgacgggc gccagcgatg
     3781 cgaagtcgcg cgacaggat
//
LOCUS       DBNHXB010000484        15544 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3011765, whole genome shotgun sequence.
ACCESSION   DBNHXB010000484 DBNHXB010000000
VERSION     DBNHXB010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 15544)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15544)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15544
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3011765"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..845
                     /locus_tag="AC162G_18625"
     CDS             177..845
                     /locus_tag="AC162G_18625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5077876.1"
                     /translation="MHTHTHTPESPAQTEGRLIRWASFYDVTVNLMTLGQASRLRRMT
                     VDQALLQPGETLLDVGCGTGGVTIPAKIRVGEGGGAAGIDPAPEMITVARRKAKRAGV
                     EIDFRVGVIESLPFPDETFDVVTSSLMMHHLPEHLRVKGLAEILRVLKPGGRILIADM
                     MRPSGSVFKQFFISLVLHHGHGAQFGIQDLPKLMTETGFEDVEQLDARFLTIGFVRAT
                     KPAA"
     gene            complement(861..1598)
                     /locus_tag="AC162G_18630"
     CDS             complement(861..1598)
                     /locus_tag="AC162G_18630"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR5077877.1"
                     /translation="MVMVTIGSRKNKGESEHSPMLTPVVVDFPLRGEWVAPNTPGKRI
                     PSHGSDMLGERYAYDFVRTGPGSRGMRFYRPSPLHYLVLGVRLQDCFGWGQPIFSATA
                     GIVVQAEDGWPERNPVHPARDLAIALKHALTFDVKRTADLRPLAGNYIIVETGDGYAV
                     YAHAQTGSIKVSPGDTVTAGQPLARVGHSGNSTAPHLHFHLMDHRDPLKAQGIPCCFR
                     EYEVLHEGVWHPVQNGIPKDTDRIRRL"
     gene            complement(1653..2474)
                     /locus_tag="AC162G_18635"
     CDS             complement(1653..2474)
                     /locus_tag="AC162G_18635"
                     /inference="COORDINATES: protein motif:HMM:NF017946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077878.1"
                     /translation="MTALAFNWRLYRRLVGAQIRSQMQYRASFLFDFVAQFVGNVVDF
                     AVVLVLFTQFPAIGGWSLGEVGLLYGLSSVSFALADMLASGFDYMYFGAAMVRQGGFD
                     QVLVRPVNAFVQVLASQFVLRRIGRIAQGALILAAALALLERAWTPDKIAFMIVTIAG
                     GIFFFAGLFIFGSGVSFWTIESLEVMNIATYGGQAMTSYPMNIYQDWLRSLFTFVIPT
                     AFVNYYPTLWLLDKPDPLGLPAWLAFLSPVVCLLVFAVGVRMWWFGVKHYQSTGS"
     gene            2613..2930
                     /locus_tag="AC162G_18640"
     CDS             2613..2930
                     /locus_tag="AC162G_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077879.1"
                     /translation="MVAPAIAATTAKIAQSLGMDESELSRQALATFLLEQKRQVLQTR
                     LEILARYGADSVSSLEARVKQGQVAEHPAWEDLIVAENLGARLEELDAYLADVQGAAS
                     ESA"
     gene            2884..3093
                     /locus_tag="AC162G_18645"
     CDS             2884..3093
                     /locus_tag="AC162G_18645"
                     /inference="COORDINATES: protein motif:HMM:NF039803.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system TumE family protein"
                     /protein_id="HIR5077880.1"
                     /translation="MPISPTYRALRQNRHDNAPHNRWRAVSSFPKHFHSGTEDNVIES
                     ALSDDPPTALREFLAFVRQKLLSVP"
     gene            complement(3101..3892)
                     /locus_tag="AC162G_18650"
     CDS             complement(3101..3892)
                     /locus_tag="AC162G_18650"
                     /inference="COORDINATES: protein motif:HMM:NF017946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077881.1"
                     /translation="MRLYLELARKSFQRQVAYRGATLAGFVTNLFFGLLRAAILVAVY
                     GARTEVAGYSIDDAITYTGLTQGFIGLTALWGWYDMIRSIRSGEVASDLSKPFDYYGF
                     WLAQDVGRGAYQWLARGVTMMIVYALLFDITAPAPAGQWLGFAISVSLAWLISFGWRF
                     LVSAAAFWATDALGFARMAYFFVLFPSGFLIPLAFMPAWLRTLCYLTPFPSMIDTPVR
                     IYLNQAQGGEVVGLIAVQGAWALALIVAGRLAAEAGRRKLTIQGG"
     gene            complement(4139..5056)
                     /locus_tag="AC162G_18655"
     CDS             complement(4139..5056)
                     /locus_tag="AC162G_18655"
                     /inference="COORDINATES: protein motif:HMM:NF048382.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HIR5077882.1"
                     /translation="MRRQLYSRLLTLFFLIAGLALLLTWNTTIQAVSSDFPATHDTYI
                     DKSSVNLNSGTAVSLDIRGHSSNTERALLAFDLSSIPSGSTVVSASLNLYFFAATSSS
                     AISINAHRVLADWDELQATWRQRLTGTNWTSAGGDYDPAVLASQSVTNTFGWVTWDVT
                     SVVQAWVSGAATNQGLILEAPSGAASEVKQFHSAECACVFEPSLQVNFEPPTPTPTDT
                     GTPTNTPTPTDTPTPTTTATPTPTASDTPTPTVTDTPTSTPTDTPTPTVTDTPTNTPT
                     ATPTLEFRTFLPLILREYSGAGTARIRFY"
     gene            complement(5181..5636)
                     /locus_tag="AC162G_18660"
     CDS             complement(5181..5636)
                     /locus_tag="AC162G_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077883.1"
                     /translation="MNGNDFFGALPASTRAHLPRHLREFKVNRRSWLVQLYYAHPLLH
                     YEVWNLGERRGELEIGLHFESRDRAENERLLAGFQSRLFEIKAELGDSIEAEQWDKGW
                     TKVYEVIPRETFTADYLEHVASRLAQWIVVAQPIFEYVNRRNQVIKRGR"
     gene            complement(5668..6204)
                     /locus_tag="AC162G_18665"
     CDS             complement(5668..6204)
                     /locus_tag="AC162G_18665"
                     /inference="COORDINATES: protein motif:HMM:NF012708.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR5077884.1"
                     /translation="MLKCPACGQESLEGELVCSNCGTFLHSGGALHTDPLEEEYQDEK
                     TKVDSSLAPPSYDTVPPGATLVLKGINDSRELVIPRKMSSVLFGRNDRLAGLVVDVDL
                     TSEDGQTRGVSRRHARIRFVNDRYLIEDLESLNGTFLNRRRLVPFVPEVLRGGDELRF
                     GSLTFTVFLRRAVESDGN"
     gene            complement(6298..7335)
                     /locus_tag="AC162G_18670"
     CDS             complement(6298..7335)
                     /locus_tag="AC162G_18670"
                     /inference="COORDINATES: protein motif:HMM:NF049034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysS/YqeB C-terminal domain-containing protein"
                     /protein_id="HIR5077885.1"
                     /translation="MSGKGLRGGDVVLFGSGETSASGRKVHEYAMSRLPAPVQVAILE
                     TPAGFQPNSALVAGKIGEFLQHRLQNYKPVVSVIPARRKDSPFSPDDPELLAPMLGAH
                     YIFLGPGSPTYAVRHLRDTLAHRYLVRRHLQGAVVTLASAAAFAAGSQVLPVYEMYKV
                     GEDPHWVAGLDLFGLYGLDLAIVPHWNNTEGGDELDTSHGFVGLERFKQLRSMLPSTT
                     HILGIDEHTAVVMDFAAGRGRVLGKGAATLCHADEDIVIPHGDSFDLAQLGPLRDAAP
                     PADFEREEPVASVELPAGAAELIEQRRAARERKDWSRSDELRDQLAALGVKVEDTRDG
                     QRWVLVPPSNG"
     gene            complement(7335..8354)
                     /gene="dusB"
                     /locus_tag="AC162G_18675"
     CDS             complement(7335..8354)
                     /gene="dusB"
                     /locus_tag="AC162G_18675"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR5077886.1"
                     /translation="MMDALTDTTLPVLETGMPIRHTRRPAFYVRDVPVHGDLILSPMD
                     GFSDRPFRLICREFGSAMSYTEFVAAEAVLHGNARTLHMLSFDPGERPIVFQFFGGDE
                     DRLEAAARRLEAMGPDIIDLNMGCSVNNVAGRGAGAGLLCDPAKVGRIFQRLTKAVSV
                     PVTAKIRLGWDDRSRNYREIARVLEDSGASLVAVHGRTKMQAYSGVADWDAIAEVKQA
                     VKIPVVANGDVRTAADVERVKRHTGCDAVMIGRAAIGNPWIFQRRDRDQVDTADQTAL
                     VRRHLALMLDFYGRERGLILFRKHVVKYVKGVRGAADLHQRMMTCEEADDFVELIAEW
                     EARGT"
     gene            complement(8351..9883)
                     /locus_tag="AC162G_18680"
     CDS             complement(8351..9883)
                     /locus_tag="AC162G_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIR5077887.1"
                     /translation="MLTSQEMPVAAPVTPEWVKDAIFYQIFPDRFARSGRVGDRLHLE
                     AWDSPPTPFGFKGGDLYGVAERLDYLQDLGVTAIYLNPIFASAANHRYHPYDYLNVDP
                     ILGGNAALRELIDAAHARNIRIVLDGVFNHASRGFWPFHHTLENGAASPYVDWFHFDP
                     DRLHGHRHFAAYPDPEAAAALRSGTGSLKAIGYQAWWDLPALPKFNTSTPAVREFLWN
                     VATHWIEFGIDGWRLDVPAEIDDDDFWREFRRRVKSANPEAYIVGEIWHEAQRWLQGD
                     QFDAVMNYPVTMASLGFYGGERLNLAETRRPTGYHDLRPLDATEFADRIDWILSLYDP
                     VITQVQLNLLDSHDTPRFITSVNGYRSALRLALLFLFTYPGAPCVYYGDEIGLDGQHD
                     PDNRKTFPWDESDWDHDLRAFVKRCVALRKAHPALRRGSVHRLHAQDGVYAFGRQLEG
                     ETLLIALNTASAMRTLSLPVGSLGFVDASLADAWTGSRYIIADGTLRDLKLAPRSGVV
                     LK"
     gene            10149..10487
                     /locus_tag="AC162G_18685"
     CDS             10149..10487
                     /locus_tag="AC162G_18685"
                     /inference="COORDINATES: protein motif:HMM:NF027875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HIR5077888.1"
                     /translation="MLTKRKLKNKVRVTFTLPAREGEESICLVGDFNNWEECVTTMAR
                     NKDGDWQVKLDLEPNREYQYRYLVNERTWRNDPAADDYVRNPFGSDNSLVSTVVTRLP
                     KRSGSKKSRG"
     gene            10550..12283
                     /locus_tag="AC162G_18690"
     CDS             10550..12283
                     /locus_tag="AC162G_18690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HIR5077889.1"
                     /translation="MSLYHRVLAFDFDGTLAKKGVVPPALRAALERLYADGYILFLVT
                     ARLFGSVDLRPLKHLMSGIVWENGAVLYHSAADEVYLPFGHIARRLVDVLEAAGVPLE
                     RGKAIVATQMPHNEAVWHAVSQWGGDAVIAYNKGAVLISPPGVSKGAGLERLLELCGF
                     SPRNLVSFGDGESDLSLLHLGELGVTVADAVPPMKEAADLVTTQPGPAGVLEALETYW
                     LNGSARTRLRPLRHERPIPLGEDEAGAVVSVSAAELAGGNLGVFGDSGSGKSWVTGLL
                     AEGMRRAGYQILLIDPEGDFKGMRALPGMVALEGDTRTLPPPAVVVALLEKMSASIVL
                     DLCSYPIDQRENYVAELLQGLRTLKACRFRPHWIVLEEAQYFLPPAGNSVSAALCPML
                     ADGGWTFVSFRPDQVAGPVLAALDRCLLTRLREPAAVQAVRRWFYGVEESLVTIPRGY
                     ARLSGERLARLRSNIRRVPHVRHLHKYLDAPLPPHKRFSFRDEQTFLGVEAASLPEFL
                     ECLPNLPMESLVYHQGRGDFTAWAEDALGDGILAAQLRKLAHRALEGEALREALLQCV
                     EAHCAELQVER"
     gene            complement(12405..12593)
                     /locus_tag="AC162G_18695"
     CDS             complement(12405..12593)
                     /locus_tag="AC162G_18695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077890.1"
                     /translation="MIRLDIQTPVGREFGALHDFQYTPTFILFDGTGVEIGRWRGRPP
                     LLRELKELEELKEPEEID"
     gene            complement(12657..12917)
                     /locus_tag="AC162G_18700"
     CDS             complement(12657..12917)
                     /locus_tag="AC162G_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077891.1"
                     /translation="MTVAQFFNQHSFLLVASVLLGGGLVVLIARRARRALWFAWFAAA
                     VIAIGVFVALRTAAPRAFETVADVQRAVSSGTPTLVEFYSDF"
     gene            complement(12914..13228)
                     /locus_tag="AC162G_18705"
     CDS             complement(12914..13228)
                     /locus_tag="AC162G_18705"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR5077892.1"
                     /translation="MKAIVYSTSWCAACRQMADFLRQQYADMETQFVPVDKMPEETQE
                     KIVAALRKLTWSDQLPVTVIGDSVVIGTDYAALIALLGPGGRPPAGGSAQHRHGIAEA
                     GA"
     gene            complement(13438..13995)
                     /gene="sigZ"
                     /locus_tag="AC162G_18710"
     CDS             complement(13438..13995)
                     /gene="sigZ"
                     /locus_tag="AC162G_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigZ"
                     /protein_id="HIR5077893.1"
                     /translation="MNRTTEDIWNEFGERLKAFILSRVSDPDDAEDILQDTFVKLHTR
                     IDTLRDGERLAPWLYQIARNTVVDYYRMRRPSVPLPETLTVEDEPSESDAESRIAASL
                     TGMVESLPDKYRQAIALTEFEGLTQQEMAARLGLSLSGAKSRVQRGRDMLRDSLLDCC
                     HFEFDRLGNLIDYTPRPDCCRHCAM"
     gene            complement(14043..14780)
                     /gene="arsM"
                     /locus_tag="AC162G_18715"
     CDS             complement(14043..14780)
                     /gene="arsM"
                     /locus_tag="AC162G_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HIR5077894.1"
                     /translation="MDNIKVEVQKRYAAIATQAGGCGCGPGDCCSSDDVTLVDYAGLD
                     ADVVEGANLGLGCGIPTRYAGIRPGDTVLDLGSGAGVDVFLAAKAVGPDGRVVGVDVT
                     PEMIARAWNNAVKGGYRNVEFRLGDIEALPVDDSSVDVVISNCVINLAPDKRRVFAEM
                     YRVMKPGGRFSISDVVTYGSVPESVRKDVELWAGCIAGATDRDEYLQIIREAGFADVR
                     IASPVEYDYPKGDGYGVMSVTVEGVKQ"
     gene            complement(15092..>15544)
                     /gene="arsN2"
                     /locus_tag="AC162G_18720"
     CDS             complement(15092..>15544)
                     /gene="arsN2"
                     /locus_tag="AC162G_18720"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HIR5077895.1"
                     /translation="MNTDRMAVAIGLARNEDISAVLTLLEQSDLPKDGLADHWATTHV
                     ARDDGRIVGSAALEMYGASALLRSVAVDPARRGQGLGQRLTRAALDLARQGGVTEVYL
                     LTETAGEFFPRFGFRAVDRAQVPEAMRQSVEFTSVCPESALTMKVRLT"
BASE COUNT         2944 a         4877 c         4912 g         2811 t
ORIGIN      
        1 cggcggctgg agcgtagcct agcgccttgt gcgtgtctgg cgtcgagggt gagatttctg
       61 aacaattcgc tcagactgtt cacaaatgaa cacatcccgc gccttgatta ttgacttcat
      121 ccgcgaacgt ggtagtctgt gcataggacg tctctaatta tactgggagt cgacaaatgc
      181 acacacatac tcatacaccc gaatcgcctg ctcaaacaga aggacgcctg atccgctggg
      241 cttcgtttta tgacgtgacg gtgaatctga tgacgcttgg tcaggcaagc cgtttacgcc
      301 gaatgaccgt ggatcaagcc ctgcttcagc ctggggaaac cctgctcgat gtaggctgcg
      361 gcacgggcgg cgtgaccatt cccgccaaaa tacgggttgg agaaggcgga ggcgcagcag
      421 gcattgaccc cgcaccggag atgatcaccg tcgcccgccg aaaggcgaag cgggcagggg
      481 ttgagattga ctttcgagtc ggcgtgatcg agtccctgcc ctttcctgat gagacgtttg
      541 acgtggtgac ttccagcctg atgatgcacc acctgcctga acacttgcgg gtcaaggggc
      601 tggctgaaat tctgcgtgtg ctcaaaccgg gtgggcggat tttgattgcg gacatgatgc
      661 gcccaagcgg atccgttttc aaacagttct tcatttccct ggtcttgcat cacgggcatg
      721 gggctcaatt tggcatccag gatctgccaa aactcatgac ggagacgggg tttgaagatg
      781 tggagcaact ggacgcccgc tttctgacca ttgggtttgt gcgagcaacg aaacctgctg
      841 cctaattcac ctagtaggca tcacagtctt cggatcctgt ccgtgtcctt ggggatacca
      901 ttctggactg ggtgccagac cccttcatga aggacctcgt actcgcgaaa gcaacaggga
      961 atcccttgtg ccttgagagg atcccggtgg tccatgagat gaaagtgtag gtgcggagcc
     1021 gtcgaattgc cggaatgccc gacacgggcc agcggttggc ccgcggtgac cgtgtcgccc
     1081 ggagagacct tgatcgagcc ggtttgagca tgggcataga ctgcgtaccc gtcgccggtc
     1141 tcgacgataa tgtagttgcc ggccagcggc cggaggtccg cagtccgttt gacgtcgaag
     1201 gtcagggcat gcttgagagc tatggcgagg tctctggcgg ggtggacggg attccgctcg
     1261 ggccagccgt cctcggcctg gacgacgatc ccggccgtag cagagaagat gggctggccc
     1321 cagccaaagc aatcctgcaa tcggacgccg aggacgaggt aatggagcgg gctgggccga
     1381 tagaaacgca tccctctgga tcccgggcct gtgcgcacga agtcgtaggc gtatctctcg
     1441 ccgagcatgt cgcttccgtg gctcgggatt ctcttgccgg gggtgttggg ggccacccat
     1501 tcgcccctga gggggaagtc tacgacgact ggagtaagca taggggaatg ctcgctctct
     1561 cccttgtttt tccggctgcc gatcgtgacc atgaccatta tgtgccaact cctgagcgcg
     1621 gcaagcaatt agccggcgag gcgccacacc cgtcacgatc ccgtgctctg gtaatgcttc
     1681 acgccgaacc accacatccg caccccgacg gcgaacacca gcaggcacac gaccggcgac
     1741 aggaacgcca gccacgccgg caggccgagc gggtcgggct tgtccaacag ccacaacgtg
     1801 ggatagtagt tcacgaacgc cgtcgggatg acgaaggtga agagactgcg cagccagtcc
     1861 tggtagatgt tcatcgggta cgaggtcatc gcctgcccgc cgtaggtggc gatgttcatc
     1921 acttcgagcg actcgatcgt ccagaacgac acgcccgagc cgaagatgaa cagccccgcg
     1981 aagaagaaga tgccgccggc gatcgtcacg atcatgaacg cgatcttgtc cggcgtccag
     2041 gcgcgctcga gcagcgcgag ggcggcggcc aggatcagcg cgccctgcgc gatgcgcccg
     2101 atgcggcgca ggacgaactg cgaggccagc acctgcacga aggcattgac cggccgcacc
     2161 agcacctggt cgaatccgcc ctgccgcacc atcgccgcgc cgaagtacat gtagtcgaat
     2221 ccggaggcga gcatgtcggc cagggcgaac gagacggacg agaggccata caacaggccg
     2281 acctcgccca gcgaccagcc cccgatcgcc gggaactgcg tgaacagcac gagcacgacg
     2341 gcgaagtcga cgacgttgcc cacgaactgc gcgacgaaat cgaacaggaa cgatgcgcga
     2401 tactgcatct gcgatcgaat ctgcgcgccg accaggcggc gatagaggcg ccagttgaat
     2461 gccagtgctg tcatgggggt taagacctgg aagtaattgg ttattggtaa ctgacgtggg
     2521 ctcgcaatga cgcaggctga gtagttactc gcaacgcacg gttattgacc gctcctacgt
     2581 gcgatgatac aatgtgccca gaggagattc acatggtagc tccagctatt gccgcgacga
     2641 cggccaagat tgcgcaatca ctcggcatgg atgagagcga actgtctcgc caggctctgg
     2701 ccaccttcct gctggagcag aagcgccagg tgttgcagac acgcctcgaa atcctggcac
     2761 gctacggcgc ggattcggta agcagcctcg aggcaagagt caagcaggga caggtagccg
     2821 aacacccggc ctgggaagac ctgatcgtgg cggagaattt gggcgcgcgc ctggaggaac
     2881 tggatgccta tctcgccgac gtacagggcg ctgcgtcaga atcggcatga caatgctcca
     2941 cacaatcgat ggcgcgcagt ctcctcattc ccgaagcatt tccattccgg tactgaagac
     3001 aacgttatcg aaagcgcgct gagcgacgat ccgccgactg ccctgcgtga gtttctggca
     3061 ttcgttcggc agaagctgct aagcgtgcct tgacgagagg ctatccgccc tgtatcgtca
     3121 gcttcctgcg gcccgcttcc gccgcgagcc ggccggccac gatcagcgcc agcgcccacg
     3181 cgccctgcac ggcaatcagc ccgacgactt ctccaccctg cgcctggttg agatagatgc
     3241 gcacgggtgt gtcgatcatg ctggggaacg gcgtcaggta acacaacgtg cggagccacg
     3301 ccggcatgaa cgcgagcgga atgagaaagc cggacgggaa gagcacgaag aagtaagcca
     3361 tgcgcgcgaa gcccagggcg tccgtcgccc agaaggctgc tgcgctgact aagaagcgcc
     3421 acccgaaact gatgagccag gccaggctga cgctgatcgc aaaccccagc cattgacctg
     3481 ccggcgccgg cgccgtgatg tcgaaaagca gcgcgtagac gatcatcatc gtcacgccgc
     3541 gcgccagcca ttgatacgcg ccgcgcccca cgtcctgcgc cagccagaag ccgtaataat
     3601 cgaacggctt cgacaggtcg gacgcgacct cgccgctgcg aatgctgcgg atcatgtcgt
     3661 accaacccca cagcgccgtc aggccgatga acccttgcgt caggcccgtg tacgtgatcg
     3721 cgtcgtcgat cgagtaaccg gccacttcag tccgggcgcc gtacacggcc accaggatcg
     3781 ccgcccgcag caatccgaag aacaggttgg tcacgaagcc ggcgagcgtc gcgccgcgat
     3841 aggccacctg gcgctggaag gacttcctgg cgagttcgag gtatagacgc atgggttgat
     3901 cagtgatcag tggccagtaa tcggtgatca gtgacacatg atgcgtggct agcgatcagt
     3961 gacgaccgcc ggcggcttga gcctaaatgt tacaccgtcg aactaaaacc gcaaaagatt
     4021 catcccaccg gatcggcagc gccggaaaca ggcagccggc tgaggccgaa tgaaatgaaa
     4081 caggcgtggg atgtaatacc acgcctgccg aagactgtca acgtctcaag atagtcgtct
     4141 agtagaaacg gattcgagca gtccccgcgc ctgaatattc gcgcagaatg aggggcagga
     4201 acgttcgaaa ctctagtgtg ggcgtagccg tcggcgtgtt ggtcggtgta tcggtcaccg
     4261 tcggcgtggg cgtgtcggtc ggcgttgatg tcggtgtgtc ggtcaccgtc ggcgtgggcg
     4321 tgtcgctcgc cgtcggcgtg ggcgtggcgg tcgtcgtcgg cgtaggtgta tccgtcggtg
     4381 tcggtgtgtt ggtcggcgtt ccagtgtccg tcggagtcgg cgtgggtggc tcaaagttca
     4441 cctgcagaga gggttcgaag acgcaggcgc actccgcact atgaaattgc ttgacctcgc
     4501 tcgcggcccc tgacggcgct tcgagaatga ggccctgatt ggtcgccgcg ccgctgaccc
     4561 aggcctggac aacgctggtc acatcccacg tcacccaacc aaaagtgttg gtcacactct
     4621 ggctggcaag gaccgccggg tcgtagtcgc cgcccgcgct ggtccagttc gtgcctgtca
     4681 ggcgctgcct ccacgtcgcc tgcagctcat cccagtcggc gaggaccctg tgagcgttga
     4741 tgcttatcgc gctgctgctc gtcgcggcga agaagtagag attgagcgaa gccgagacga
     4801 ccgtcgagcc gctcgggata ctggagagat caaaggcgag caaggcgcgc tcggtgttac
     4861 tgctgtgtcc tcggatatcg agcgacacag cagtgccgga attcagattc acgctgctct
     4921 tatcaatata ggtatcatgc gtcgcgggga agtcggacga tacggcctgg atggtcgtat
     4981 tccacgtcag gagaagagcc aggcccgcga tgaggaaaaa aagagtgaga agtctgctat
     5041 atagctgccg tctcatgaga tggcccccct aagcgataca gcatattcta gtgtcgcatt
     5101 atacaacagg caggcttcgt gggtgtttgt aatcgcaggt tatttagtta ggtttcggtc
     5161 aaaatatctt gacaaaccgg ttagcgcccg cgctttatca cctgatttcg cctgttgaca
     5221 tattcaaaga tcggctgcgc gacgacgatc cactgcgcca gccgagacgc cacatgctcg
     5281 agataatccg ctgtgaacgt ctcgcgcgga atcacttcgt agactttcgt ccagcctttg
     5341 tcccactgct cggcttcgat cgagtcgccc agctccgcct tgatctcgaa caggcgcgat
     5401 tggaagccgg cgagcaggcg ctcgttctcg gcgcgatcgc ggctctcgaa atgcagcccg
     5461 atctccagct cgccgcgccg ctcgcccagg ttccacacct cgtaatgcag caagggatgg
     5521 gcatagtaca actgcaccag ccagcttcta cggttgacct tgaattcgcg caggtgtcgc
     5581 gggaggtgcg cgcgcgtcga agccggcagc gcgccgaaga agtcgttgcc attcaagtcg
     5641 cacgctccag ccgaaggcaa acgccgctca gttgccgtcg gactcgacag cgcgtctgag
     5701 gaacacagtg aaggtcagcg agccgaaccg aagctcgtcg ccgccgcgca gcacttcggg
     5761 gacaaagggg accaggcgcc gccgattgag aaacgtgccg ttcaggcttt ccaggtcttc
     5821 gatcagatac cgatcgttca cgaagcgaat ccgcgcgtgg cgtcgcgaca cgccgcgcgt
     5881 ctgcccgtct tcgctcgtca gatcgacgtc gaccaccaac ccggccaggc gatcgtttcg
     5941 cccaaacagc acgctcgaca tcttgcgcgg gatgaccagc tcgcgactgt cgttgatccc
     6001 tttcaggacc agcgtcgcgc cggggggaac cgtgtcgtag ctcggcgggg cgagtgacga
     6061 atcgactttc gtcttttcgt cttgatactc ctcttccagt ggatcggtgt ggagcgcgcc
     6121 gccgctgtgc aggaacgtgc cgcagttgct gcacaccagc tcgccttcca acgattcctg
     6181 gccgcaagca gggcacttga gcataggcaa ctcgctgctg gcgagattca atatcattgt
     6241 aatacgattt cacgcgggcg caaatccaca ccgtgccagt gtgcacgcaa aggttgatca
     6301 accgttcgag ggcggcacga ggacccagcg ctgaccgtct cgcgtgtcct cgaccttcac
     6361 gccgagcgcc gccaattgat cgcgcagttc atccgagcgg gaccagtcct tcctttcgcg
     6421 cgccgcgcgc cgctgctcga tcagctcggc cgcgcccgcc ggcagctcga ccgaagcaac
     6481 cggctcttcc cgttcgaaat cggcaggcgg cgctgcgtcg cgcagcgggc cgagctgcgc
     6541 caggtcgaat gaatcgccgt gggggatcac gatatcttca tcggcgtggc acaacgtcgc
     6601 cgcgcctttg cccagcaccc ggccgcggcc ggccgcgaaa tccatcacga ccgcggtgtg
     6661 ctcgtcgatg ccgaggatgt gggtggtcga aggcagcatg ctgcgcaact gcttgaagcg
     6721 ctccaggccg acgaagccgt gggacgtatc gagctcgtcc ccgccttccg tgttgttcca
     6781 gtgcgggacg atcgccagat caagcccgta cagtccgaac agatccagcc ccgccaccca
     6841 gtgcgggtct tcgccgacct tgtacatttc gtacaccgga agcacctgcg aaccggcggc
     6901 gaacgcggcg gcgctggcca acgtgaccac cgctccctgc aaatgccgcc gcaccaggta
     6961 gcgatgggcc agcgtgtccc gcaggtggcg caccgcgtac gtcgggctgc ccgggcctag
     7021 gaagatgtaa tgcgcgccga gcatcggggc gagcagctcg ggatcgtcgg ggctgaaggg
     7081 gctgtctttt cggcgcgcgg gaatgaccga aacgacgggc ttgtagttct gcaggcggtg
     7141 ttgaaggaac tcgccgattt tgccggcgac gagagccgaa ttcggctgga agccggccgg
     7201 cgtctcgagg atggcgacct gcaccggcgc aggcagccgg ctcatggcgt attcgtgcac
     7261 cttgcgcccg ctcgccgacg tctcgcctga gccgaacaac accacgtcgc cgccgcgcaa
     7321 tccctttcct gacatcacgt gccgcgcgcc tcccactcgg caatcagctc gacgaaatcg
     7381 tcggcctctt cgcacgtcat catgcgctgg tggaggtctg ccgcgccgcg cacgcccttg
     7441 acgtacttga cgacgtgctt gcggaacagg atcaggccgc gctcgcggcc gtagaagtcg
     7501 agcatcagcg ccaggtggcg gcgcaccagc gccgtctggt cagccgtgtc gacctgatcg
     7561 cgatcgcgtc tctggaagat ccacgggttg ccgatcgccg cgcggccgat catcactgca
     7621 tcacatccgg tgtgccgctt gacgcgctcg acgtcggccg ccgtccgcac gtcgccattg
     7681 gcgacgacgg ggatcttcac cgcctgcttc acttcggcga tggcgtccca gtcggcgacg
     7741 ccggagtagg cctgcatttt cgtccgcccg tgcacggcga cgagcgaagc gccgctatcc
     7801 tccagcacgc gggcgatctc gcgatagttg cgcgagcggt cgtcccagcc cagacggatc
     7861 ttggccgtga ctgggaccga gacggccttc gtcaggcgct ggaagatgcg cccgaccttg
     7921 gccgggtcac acagcagccc ggcgcccgcg ccccggccgg cgacgttatt caccgagcag
     7981 cccatattga gatcgatgat gtcaggcccc atcgcttcca gccggcgcgc cgcggcttcg
     8041 agcctgtctt cgtcgccgcc gaagaactgg aacacgatcg ggcgctcgcc cggatcgaac
     8101 gagagcatgt gcagcgtcct cgcgttgccg tgcaagacgg cctccgccgc gacgaactcg
     8161 gtgtacgaca tggccgagcc gaactcgcgg cagatcaggc gaaagggcct gtctgagaag
     8221 ccgtccatcg gcgacaggat cagatcgccg tgcacaggca catcccggac atagaaagcc
     8281 gggcggcggg tgtggcgaat gggcataccc gtttccagca cgggcagtgt ggtatcggtc
     8341 agcgcatcca tcatttcaag acgacgccgc tgcgcggagc gagtttcagg tcgcgcagcg
     8401 ttccatccgc aataatatat cgcgatcccg tccaggcgtc agccagcgat gcgtcgacga
     8461 agcccaggct cccgacaggc aggctcagcg tgcgcatggc cgacgcggta ttcaacgcga
     8521 tcaggagcgt ctcgccctcg agctggcggc caaacgcata cacgccgtcc tgcgcatgca
     8581 ggcgatgcac cgatccgcgc cgcagcgccg ggtgcgcttt gcgcagcgcc acgcatcgtt
     8641 taacgaacgc ccgcaggtcg tgatcccagt ccgactcatc ccacgggaag gtcttgcggt
     8701 tgtcgggatc gtgctggccg tccaatccga tttcatctcc atagtaaacg cacggcgcgc
     8761 cgggataggt gaacaggaag agcagcgcca gccgcagcgc cgagcgatag ccgttcacac
     8821 tggtgatgaa gcgcggcgtg tcgtggctgt ccagcaggtt caactgaacc tgcgtgatca
     8881 ccggatcgta cagactcaga atccagtcga tgcgatcggc gaactcggtc gcgtcgagcg
     8941 gccgcaggtc gtggtatccg gtggggcggc gcgtctccgc caggttcaga cgctcgccgc
     9001 cgtagaagcc caggctggcc atggtgaccg ggtaattcat caccgcgtcg aactgatcgc
     9061 cctgcagcca gcgctgcgcc tcgtgccaga tctcgccgac gatgtaggct tcgggattgg
     9121 ccgatttgac gcggcggcgg aactcgcgcc agaagtcatc gtcgtcgatc tcagccggca
     9181 catccagccg ccaaccgtcg atgccgaatt cgatccaatg cgtggcgacg ttccagagga
     9241 actcgcgcac ggctggtgtg ctcgtgttga acttgggcag cgcgggcaga tcccaccacg
     9301 cctggtagcc gatggctttg agactgcccg ttccactgcg cagcgccgct gccgcttccg
     9361 gatcgggata cgcagcaaag tgtctgtggc cgtgcagccg atccggatcg aaatggaacc
     9421 aatcgacgta gggtgacgca gcgccgttct ccagcgtgtg gtggaacggc cagaagcccc
     9481 ggcttgcgtg gttgaacacg ccgtcgagca cgatgcgaat attccgcgcg tgcgccgcgt
     9541 cgatcaactc acgcagcgcg gcattgcccc ccaggatcgg atcgacgttg aggtagtcgt
     9601 aggggtgata acgatgattg gccgccgaag cgaagatcgg gttcaagtag atcgcggtga
     9661 cgcccaggtc ttgcaagtaa tccaggcgct cggcgacgcc gtacagatcg ccgcctttga
     9721 agccgaacgg cgtcggcggg ctgtcccatg cttccaggtg aaggcgatcc cccacgcgcc
     9781 cgctgcgcgc gaagcggtcg gggaagatct gatagaaaat ggcgtcttta acccattcag
     9841 gtgtgacggg cgcagcaacc ggcatctcct gcgaggtcaa catgaaggct ccctgaagcg
     9901 gtaatttgct tgaggggtga ttatatcatg gtgaagcgcg ctgcaaagag gaagcgtgct
     9961 ttgcgtcaca aacgcaaagg tattgactgc gccttccgat ccgtgctaca ctgtgaatgc
    10021 ccgtcaaata taccagaacg accgccgcac cggaccgaag cgcgagagtt atgcgccttc
    10081 agtaggtgct tattttgttt tagaggcaag tttgcaattg gtttgagctt tatatggagg
    10141 atgacgacat gctcacgaaa cgcaagctca agaacaaagt gcgtgtaacg ttcaccctgc
    10201 cggcccgcga gggagaagag tcgatctgcc tggtcggcga cttcaacaat tgggaagagt
    10261 gcgtcacaac gatggcccgg aacaaagatg gcgattggca ggtgaaactc gatctcgagc
    10321 cgaaccgcga gtaccagtat cgctacctgg tgaacgagcg cacctggcgt aacgacccgg
    10381 ctgccgacga ctacgtgcga aatccgttcg ggtcagacaa ctcactggta tccaccgtcg
    10441 tcacgcgcct gccgaagcga agcggctcta agaaatcccg tggttgacgc atccccatgc
    10501 gtcaacagcg ggcatgggga tgcccgttct acgggtttta acgcgaagca tgagcctata
    10561 tcaccgcgtg ctggcttttg acttcgacgg caccctggcc aagaaggggg ttgtgccgcc
    10621 ggcgctgcgt gctgcgttgg agcggttgta cgcggacggg tacatcctct tcctggtcac
    10681 cgcacgcctg ttcggcagcg ttgacctgag accgctgaag cacctgatgt caggcatcgt
    10741 gtgggaaaac ggcgcggtgc tctaccacag cgccgccgac gaggtttacc ttccctttgg
    10801 ccacatcgcc cggcgcctgg tggacgtatt ggaagccgct ggagttcctt tggagcgcgg
    10861 gaaagccatc gttgccacgc agatgcctca taatgaggca gtctggcatg ccgtcagcca
    10921 gtggggtgga gacgcggtga tcgcgtacaa caagggcgcg gtgctgatct cgccgccggg
    10981 cgtgtccaaa ggcgccgggc ttgagcggct cctggagctg tgcggttttt cgccgcgcaa
    11041 cctggttagc ttcggcgacg gggagagcga tttgtcactg ctgcacttgg gagaactcgg
    11101 cgtgactgtc gcggacgccg taccacccat gaaagaggca gccgacctgg tgacgaccca
    11161 gcccggtccg gcaggcgttt tagaagccct ggaaacctac tggttgaatg gatccgcccg
    11221 cacaaggctc cgccccttga gacatgagcg ccccattccg ctgggcgaag acgaggccgg
    11281 cgctgtggtg tccgtgtcgg ccgccgaact ggccggcggg aacctgggcg tgttcggcga
    11341 ttcgggcagc ggcaaatcgt gggtgaccgg attgttggcc gaaggcatgc gacgcgccgg
    11401 ctaccagatc ctgctgatcg atcctgaggg cgacttcaag gggatgcgtg cgctgcctgg
    11461 catggtggct ctcgagggag acacgagaac acttccgccc ccggcagtgg tcgtcgcgct
    11521 gctggagaag atgagcgcat cgatcgtgct cgacctgtgc tcgtacccga tcgaccagcg
    11581 cgagaattac gtcgccgagc tgctgcaagg tctgcgcact ttgaaggcct gccggttccg
    11641 gccccactgg atcgtgctgg aggaagcgca gtactttctg ccaccggccg gtaactcggt
    11701 ttccgccgcg ctgtgcccga tgctcgccga cggcggatgg accttcgtct cattccgccc
    11761 cgatcaggtg gccggcccgg tgctggctgc gctcgaccgt tgtctgctga ctcgcctgcg
    11821 cgaaccggcg gccgtgcagg ccgtgcgccg atggttttat ggcgtcgaag agtctctcgt
    11881 gactattccc cgaggatacg cccggttgag cggcgagcgc ctggcgcgcc tgcgctccaa
    11941 tatccgccgc gtgccacacg tccggcatct gcacaaatac ctcgacgctc ccctcccccc
    12001 acacaagcgc ttcagctttc gcgacgagca aacctttctc ggcgtggagg ctgccagcct
    12061 gcccgagttt ctagagtgcc tgccaaattt accgatggag agtctggttt atcaccaggg
    12121 gcgcggcgac ttcacggcgt gggcggagga cgcactcggc gatggcatcc tggcggccca
    12181 actgcgcaaa ctcgctcacc gcgcactcga gggcgaggcc ctgcgcgagg cgctgctgca
    12241 gtgcgtggag gcccactgcg ccgagttaca ggtagagcgc tgacaggtgc cattgaaaac
    12301 cactcggtac catccgcaaa ccctcggagt gcggggcttt agcccgcttt ggtcgcgcag
    12361 actaaagtct gcactccact ttcaagacaa ggcggggcag attatcaatc gatttcctcc
    12421 ggctccttca gttcctccag ttccttcagt tccctcagga gcggcggcct cccgcgccat
    12481 cgcccgatct ccacgccggt tccatcgaac agaatgaacg tcggcgtgta ctggaaatcg
    12541 tgcagcgcgc cgaattcgcg cccgaccggc gtctggatgt cgaggcgaat cacctgcccg
    12601 gctctccaac tgcgctccag ctcgtcgacg acgggcttgg ccgagaggca ggccagtcag
    12661 aaatccgaat agaactcgac gagggtgggc gttccagagg ataccgcacg ctgcacgtcg
    12721 gcgaccgtct cgaatgcgcg cggcgcggcg gtgcgcagtg cgacgaagac gccgatcgca
    12781 atcacggcgg cggcgaacca ggcgaaccac agagcgcggc gcgcccggcg cgcgatcaag
    12841 acgaccagcc ctccgccgag cagcacgctc gccaccagga ggaacgagtg ctggttgaag
    12901 aactgggcga ccgtcacgcg cccgcctcgg cgattccgtg ccggtgctgc gcgctgcctc
    12961 cggcgggcgg cctgccgccg ggtcccagca gggcgatcaa ggcggcgtag tccgtgccga
    13021 tgacgacaga atcgccgatc acggtgacgg gcaattgatc cgaccaggtg agcttgcgca
    13081 gcgccgcgac gatcttctct tgcgtttcct ccggcatctt atcgacgggg acgaattgcg
    13141 tctccatatc ggcgtactgc tgccggagga agtcggccat ctggcggcag gcagcgcacc
    13201 acgaagtgct gtagacaatc gctttcatgt gaacgccgga aggacgcgcg ctactccccc
    13261 ctcggccctg ctcagggcat ggctcagggt gcggctgaca ggttttgctt gcacacaggc
    13321 tggccgaatt gtatcacaat cgcggttgac agccccaggg accacggagg ctctccttca
    13381 atgctccgcg ttctcagtgt ctgcgtggtt ttctgttcgc gatggagagg ccgagcgcta
    13441 catagcgcag tggcggcagc agtcggggcg gggtgtgtag tcgatgaggt tgcccaggcg
    13501 gtcgaattcg aagtggcagc agtcgagcag cgaatcgcgc agcatatccc gtccgcgctg
    13561 cacgcgtgac ttcgcgccgc tgagcgacag ccccagccgc gcagccatct cctgctgcgt
    13621 gagtccttcg aactcggtga gggcgatggc ctggcggtat ttgtccggca gggactcgac
    13681 catgccggtc agcgaggcgg cgatgcgcga ttctgcgtcg ctttcggatg gctcgtcttc
    13741 gacggtcagc gtctccggca ggggaacgga cgggcgccgc atgcggtagt aatccacgac
    13801 cgtgttgcgc gcgatctggt acagccacgg cgcgagccgc tcgccgtcgc gcagcgtatc
    13861 gatgcgcgtg tgcagcttga cgaaggtgtc ctgcaggatg tcctcggcgt cgtcgggatc
    13921 ggaaacacgg ctgaggataa acgccttcag ccgttcgccg aattcgttcc agatgtcttc
    13981 ggtcgtgcgg ttcatattgc caagacttcg gaagtctcaa aaagacttcc gaagtctgat
    14041 catcactgct tgacgccctc gacggtgacg ctcatcacgc cgtaaccgtc gcctttcgga
    14101 taatcgtact cgaccgggct ggcgatcctc acgtccgcga aaccggcctc gcgaatgatc
    14161 tgcaggtact cgtcgcggtc cgtcgcgccg gcgatgcagc ccgcccaaag ctcgacgtct
    14221 ttacggaccg actccggcac gctgccgtaa gtcacgacgt cggagatcga aaaccggccg
    14281 cccggcttca tcacgcggta catctcggcg aacacgcggc gtttgtccgg cgccaggttg
    14341 atcacgcagt tggagatcac gacgtcgacg ctgctgtcgt cgaccggcag ggcttcgatg
    14401 tcgcccagcc ggaactcgac gttgcgatag ccgcctttga cggcgttgtt ccaggcgcgg
    14461 gcgatcatct ccggcgtcac gtcgacgccg accacgcgcc cgtccgggcc gacggccttc
    14521 gcggccagga aaacgtccac gcctgcgccg ctgcccaggt cgagcacggt atcgcccggc
    14581 cgaataccgg cgtaccgtgt cgggatgccg cagccgaggc cgagatttgc gccctcgacc
    14641 acgtctgcgt cgaggccggc gtaatcgacg agcgtcacat cgtcacttga gcagcaatcg
    14701 ccgggaccgc acccgcagcc cccggcctgc gtcgcgatgg ccgcgtacct cttttgcact
    14761 tcgactttga tgttatccat actggactcg ctttcagacc tgacaggtca cttcgctgcc
    14821 tcagcagcaa tcacagccgc agccacaatc acaatcggag cagcagtcgc agccgcagtc
    14881 gtcgggctgc gtggggcctg gtgtgtaccg tttcggaaac atggcctttc ctcccttgaa
    14941 tggtctcaac cttatagacg gaggcgacgc gaaaaggacg caaaatgccg ccgggcggct
    15001 aagagcctat ctgaaaaccc tagcggaggc ggctcccatg ccgccggatg gctgggcgtg
    15061 ggagcccagc ccgctagctt ccagataagc actaggtcag ccgcaccttc atcgtgagcg
    15121 cgctctcggg acagactgac gtgaactcga ccgactgccg catggcttcc ggcacctgcg
    15181 cgcgatcgac cgcgcggaag ccgaagcgcg ggaagaactc gcccgccgtc tcggtcagca
    15241 ggtagacctc cgtcacgcct ccctggcgag ccagatcgag cgccgcccgc gtgagccgct
    15301 gacccagacc ctgcccgcgc cgcgccggat cgaccgccac cgagcgcagc agggccgacg
    15361 cgccgtacat ctccaatgcc gcactgccga cgatccggcc atcgtcgcgc gcgacgtgcg
    15421 tcgtcgccca gtgatcggcc aggccatcct tcggcaggtc gctttgttcg agcagcgtca
    15481 ggacggcaga tatgtcctca ttacgggcga gtccgattgc gactgccata cgatccgtgt
    15541 tcat
//
LOCUS       DBNHXB010000485         5429 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_3179090, whole genome shotgun sequence.
ACCESSION   DBNHXB010000485 DBNHXB010000000
VERSION     DBNHXB010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_3179090"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1028)
                     /locus_tag="AC162G_18725"
     CDS             complement(42..1028)
                     /locus_tag="AC162G_18725"
                     /inference="COORDINATES: protein motif:HMM:NF016983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HIR5077896.1"
                     /translation="MATTPSSKKQAEEIAARLAPDARQLDAAGANRALRFFLHRIAEV
                     DRAEVAARVNELRQCYPNAAPDELAEKLVKAKCQKTATVGAVSSGGALLPGIGSLVAG
                     AAGLVADLRTTITLQAELVLEIAGVYGVPIDDADRQQVVMLLTGIGYGTSHLLTTAGS
                     QLTGKLAARVTRNWLARALPIIGVAASASTNALSTYIVGQRARAYFSHGPEAIGDWAD
                     TLRLITGVDERKVAGWLAERSVEARDTVGGAAVSASAALETVKALMQRSATEAGDRLA
                     RGVRSVSRTIEQAGARAGKEARHIGDAIGTASESVKSTARSAARRRRRDRKL"
     gene            complement(1040..3124)
                     /locus_tag="AC162G_18730"
     CDS             complement(1040..3124)
                     /locus_tag="AC162G_18730"
                     /inference="COORDINATES: protein motif:HMM:NF014516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5077897.1"
                     /translation="MIPLRLHLRNFMPYADAAIDFRGIRVGCIAGDNGVGKSALLDAI
                     TWSLWGRARARRDDELVRQGAPEMAVTFAFGLGGNTYQVIRTRKTGKRGAGALDVQVV
                     HDGEWKTIAEPTISQTQAKINTLLRLDYDTFINSSFLLQGRADEFTVKTPADRKKVLG
                     DILGLGAWEACEERAKERIRAIESSMQAVDIRLREIDDEIARRPQYEAEVAGAQKAVI
                     ELGGALRQVEIRYQKVQQARQELRHVEAQTAELSNRARHADRELNAVLAELETARLKA
                     DTTAIQGELDTVRADLVRIAEVESLRDASRARRNALAEDSAALKGQNDRLGPETEPLK
                     QRVELLAAATEPTCPTCGQPLSPDHREHVIAGLESEIEHRRELYRANVQRLNAIKAEL
                     ASLDRELAALDGDLRRQPPLQRREAELEAALAAAAEARKSIATLEARCEQWQKLYDAD
                     SMKQAEAAAKAAALRAELGDASAIEAEYERTRAEEARARQLLGAAQQKLAACDALVNA
                     REGKVAERNRLATEKGIYEELRLAFGKKGVPAMIIEAAIPEIEDEANALLSRMTGGRM
                     RIRFDTQKETLKGDTVETLDIQIADELGTRPYENFSGGEQYRINFAVRIALSKLLARR
                     AGAQLQTLVVDEGFGALDASGRERLVEAINAVQSDFERILVITHLDELKDTFPTRIDV
                     TKTENGSQIAIM"
     gene            complement(3132..3425)
                     /locus_tag="AC162G_18735"
     CDS             complement(3132..3425)
                     /locus_tag="AC162G_18735"
                     /inference="COORDINATES: protein motif:HMM:NF039814.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5647 family protein"
                     /protein_id="HIR5077898.1"
                     /translation="MNSFYQAKFDELFTEFTRYLTEHPEFAERIPENAEVVLLDKNDP
                     QYSQYAIDLSKRSRLTDDEPDRPKVYIEVTEMAPVRSRVRKLLVHERPPAYTT"
     gene            complement(3422..3742)
                     /locus_tag="AC162G_18740"
     CDS             complement(3422..3742)
                     /locus_tag="AC162G_18740"
                     /inference="COORDINATES: protein motif:HMM:NF049837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7718 family protein"
                     /protein_id="HIR5077899.1"
                     /translation="MSERAFITYLDETRENRYRHWHSWDRGKIIEFRIQYEALISGHW
                     HSVVRYDSAHGQPHRDRLHPDGTQTKDWFPSYSNAEVLTIGQRDIRENWPKYRAQYEK
                     EMQG"
     gene            complement(3775..5424)
                     /locus_tag="AC162G_18745"
     CDS             complement(3775..5424)
                     /locus_tag="AC162G_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA domain-containing protein"
                     /protein_id="HIR5077900.1"
                     /translation="MEDRPAASRLGVVVGGSLSKGLEVKLDRTADIESTAVGRYVVVE
                     GRSRRFFSMITDVSLDATDPRIAKLPPDLSDDFTREVMIGTNVFGAIHVTPMLMLDAQ
                     AGATEPKPVKTVPGHFAPVVEASAEDVNLIFGSSKDEGYFHVGEPLDMEDIPIYISLR
                     RFVERSSGVFGKSGTGKTFLTRMLLAGIVKENVAVNLVFDMHNEYGWKSQSESRAEVK
                     GLRQLFPSSRVAVATLDDESSRRRGSKVDSVVQIGYDQLEPEDVEMLAGLMGLSDVQV
                     GALHAIYRRLGRNWVARLLGDERPEEMQAMIDAGLIHEGTLGAIQRKLSRFQDFGFLK
                     PSVTANAVEELLGYIEQGKHVVLEFGRYGSSLEAYILVANYLTRRIHQVYVRRKEAAL
                     GSQADEPRPLVITIEEAHKFLDPAIAGQTIFGTIARELRKYNVTLLIVDQRPSGIDSE
                     VMSQIGTRVTCLLDDESDIRAVFSGVSGAGELRNVLAKLDTRQQALILGHAVPMPVVV
                     RTRDTMTPDYYASLGYVEGEDLQKKLARNKRALGRDGGGLD"
BASE COUNT          827 a         1838 c         1665 g         1099 t
ORIGIN      
        1 ataacatata ggtgtgtcat ctagcgacac atctcccgcg ctcagagctt cctgtcccga
       61 cgtcgtctgc gagcggcgct ccgggcggtc gacttcacac tctcggacgc ggttccaatc
      121 gcatctccaa tgtggcgcgc ctccttgcca gcgcgtgcgc ccgcctgctc gattgtccgg
      181 gacacgcttc gcacgccacg ggccaggcgg tcgccggctt ctgtagcgga tcgctgcata
      241 agcgccttca cggtctccag cgcggcgctg gccgacacgg cagcgccgcc aaccgtgtcc
      301 cgcgcttcca cgctccgctc ggccagccag ccggcgacct tgcgctcgtc gacgccggta
      361 atcaggcgaa gcgtgtcggc ccagtcgccg atcgcctctg gcccatggct gaagtaggcc
      421 ctggcgcgct ggccgacaat ataggtggac agcgcgttgg tgcttgccga ggcggccacg
      481 ccgatgatcg gcaaggcgcg ggccagccaa ttgcgggtga ctcgcgcggc caacttgccc
      541 gtcaattggg agccggccgt ggtgagcaag tgactggttc catacccgat gcccgtcagc
      601 agcatcacca cctgctggcg gtctgcgtcg tcgatgggca ctccgtagac cccggcgatc
      661 tcaagcacca gctcagcctg cagggtgatc gtcgttctca agtcggcgac cagaccggct
      721 gcccctgcta cgagcgagcc gatgcccggc agtaatgcgc cgccggacga caccgcgccg
      781 accgtcgccg tcttctggca cttggctttg accagcttct ctgccagctc atccggcgcg
      841 gcgttggggt aacattgtct taactcgttc acgcgcgccg cgacttccgc ccggtcgact
      901 tcggcgatac ggtgaagaaa gaatctcagc gcgcgattcg cgccggcggc gtcgagctgc
      961 ctggcgtctg gcgcgagccg ggcggcgatc tcttcggcct gtttcttgct ggagggtgtc
     1021 gtggccatgc tgagaccaat cacatgatcg cgatttgaga cccgttttct gttttggtca
     1081 cgtcgatgcg cgtcgggaaa gtgtccttca gctcgtccag gtgcgtgatc accagaatgc
     1141 gctcgaagtc tgattgcacg gcgttgatcg cctcgaccag ccgctcgcgg cctgacgcgt
     1201 cgagcgcgcc gaagccctcg tcgacgacga gcgtctgaag ctgtgcgccg gctcgccgcg
     1261 cgagcagctt gctcagcgct atgcgcaccg cgaagttgat ccggtattgc tcgccgccgc
     1321 taaaattttc atagggccgc gtacccagct cgtctgcgat ctggatgtcg agcgtctcga
     1381 cggtgtcgcc tttcagcgtc tccttctggg tgtcgaagcg aatgcgcatt cgcccgcccg
     1441 tcatcctgct cagcagcgcg ttggcttcgt cttcgatctc ggggatcgcc gcctcgatga
     1501 tcatcgccgg cacacccttc ttgccgaagg ccaggcgcag ctcttcgtag atgcctttct
     1561 ccgtggccag gcggttgcgc tcggcgacct tcccttcacg cgcgttgacg agcgcgtcgc
     1621 atgcggcaag cttctgctgg gccgcgccga gcaactgccg cgcgcgcgct tcctcggcgc
     1681 gtgtccgctc gtactccgct tcgatcgcgc tggcatcccc cagctcggcg cgcagcgccg
     1741 ccgccttggc cgctgcctca gcctgcttca tcgaatcggc gtcgtacagc ttctgccact
     1801 gctcgcaccg cgcttcaagc gtagcgatcg atttgcgcgc ttcggcggcc gcggccagtg
     1861 ccgcttccaa ttccgcctcg cggcgctgaa gtggcggctg ccggcgcagg tcgccgtcga
     1921 gcgcggcaag ctcgcgatcg agcgacgcca gttcggcctt gatggcgttg agccgctgca
     1981 cattggcgcg gtaaagttcc cggcgatgct cgatctccga ctccaggccg gcgatcacgt
     2041 gttcccgatg gtcgggggag agcggctggc cgcaggtcgg gcaggtcggc tcagtcgccg
     2101 ccgcgagcag ctcgacacgc tgtttgagcg gctccgtctc cggcccgagg cgatcgtttt
     2161 gacccttgag cgcggcgctg tcctccgcca gcgcatttcg ccgcgcgcga gatgcatcgc
     2221 gtagagactc gacttcagct atccggacca gatcggcgcg caccgtatca agttcgccct
     2281 ggatcgccgt cgtgtcggcc ttgaggcgcg cagtctcaag ctcggccagc acggcattaa
     2341 gctcgcggtc ggcgtggcgc gcccggttcg atagctccgc cgtttgcgct tcgacatggc
     2401 gcaattcttg ccgggcctgc tgcaccttct gataacggat ctcgacctgc cgcagcgcgc
     2461 cccccagctc gatcacggcc ttttgcgccc ctgcaacctc ggcctcgtat tgcggccggc
     2521 gcgcgatctc gtcgtcgatc tcgcgcaggc ggatgtcgac tgcctgcatg ctcgactcga
     2581 tcgcgcggat gcgctctttg gcacgctcct cgcacgcttc ccacgcgccc aggccgagga
     2641 tgtcgcccag caccttcttg cgatcggccg gcgtcttgac cgtgaactcg tcggcgcggc
     2701 cctgcagcag gaacgacgag ttgatgaacg tgtcgtagtc gagccggagc agggtgttga
     2761 tcttcgcctg cgtctggctg atcgtcggct cggcgatcgt cttccactcg ccgtcgtgaa
     2821 ccacctgcac gtccagcgcg cctgcccccc gcttgcccgt cttgcgcgtg cgaatcacct
     2881 ggtatgtatt gccgcccagc ccgaatgcga acgtgacggc catctccggc gcgccctggc
     2941 gcaccagctc gtcgtcgcgc ctggcccgcg cgcggcccca caacgaccat gttatggcgt
     3001 cgagcagcgc gctcttgccc acgccgttgt cgcccgcgat gcagccgacg cgaatgcccc
     3061 ggaagtcgat cgccgcgtcg gcgtagggca tgaagttgcg caggtggagt ctgaggggga
     3121 tcattgagcc atcaggttgt gtaggcagga ggccgttcgt ggaccaggag cttccttaca
     3181 cgcgagcgca ctggggccat ttccgttact tcgatataga ccttcggccg gtcaggctcg
     3241 tcgtcggtta acctcgagcg tttggataga tcgatcgcgt actggctgta ttgaggatcg
     3301 ttcttgtcga gtaacacaac ttcggcgttc tccggaatac gctcggcgaa ttcgggatgt
     3361 tcggtaaggt agcgagtgaa ctcggtgaac agttcgtcga acttggcttg gtagaacgaa
     3421 ttcatccctg catctccttc tcgtactgcg ctcgatactt cggccaattc tcccgaatgt
     3481 ctctttgtcc aattgtcagc acttcggcgt tactgtagct gggaaaccaa tccttcgtct
     3541 gagtgccatc cgggtgaagc ctatctcgat gaggctgacc gtgagcgctg tcgtatctta
     3601 ccactgaatg ccagtgtcct gaaatgagcg cctcgtactg aattcgaaac tcgatgatct
     3661 tgccgcgatc ccagctatgc cagtgacggt agcggttctc gcgcgtttca tcgagatagg
     3721 tgatgaatgc ccgttcgctc acctgtcacc ttgcccgatt gagactcgcg tcgatcaatc
     3781 cagtccgccg ccatcacgcc cgagcgccct cttgttgcgt gcgagtttct tctgcaagtc
     3841 ctcgccttcg acgtagccga gcgatgcgta gtagtccggc gtcatcgtgt cgcgcgtgcg
     3901 gacgacgacc ggcatcggca cggcgtggcc gaggatgagc gcctgctgcc gcgtatccag
     3961 cttcgccagc acgttacgca gctcgcccgc gcccgacacg ccggagaaca cggcccggat
     4021 gtccgactcg tcgtcgagca ggcaggtcac gcgcgtgccg atctgtgaca tgacttcgga
     4081 atcgatgccg ctcggcctct gatccacgat caagagcgtg acgttgtact tgcgcagctc
     4141 gcgcgcgatc gtaccgaaga tcgtctgtcc ggcgatagcc ggatcgagga acttgtgcgc
     4201 ttcctcgatg gtgatgacga gcgggcgcgg ctcatcggcc tggctgccca gcgccgcttc
     4261 cttgcgccgg acgtatacct ggtgaatgcg gcgcgtcaga tagttcgcca cgaggatgta
     4321 cgcttcgagc gagctgccgt accggccgaa ctccagcacg acatgcttgc cctgctcgat
     4381 gtacccgagc agttcttcga ccgcattcgc cgtcacgctc ggcttcagga atccgaagtc
     4441 ttgaaagcga ctgagcttac gctgaattgc tcccagcgtg ccttcgtgga tcaggcccgc
     4501 atcgatcatg gcctgcattt cttcgggccg ctcgtcgcca agtagacgcg ccacccagtt
     4561 gcgcccgagc cgccggtaga tcgcgtgcag cgcgccgacc tgcacgtcgc tcagacccat
     4621 cagcccggcg agcatctcga cgtcttccgg ctccaactgg tcgtagccga tttgcacgac
     4681 agagtcgacc ttacttccgc gccgccggct ggactcgtcg tcgagcgtgg cgacggccac
     4741 ccggctcgaa ggaaacaact gccgcaggcc cttcacctcg gctctcgatt cgctctgcga
     4801 cttccagccg tactcgttgt gcatgtcgaa caccaggttg acggcgacgt tctctttgac
     4861 gatgccggcc agcagcatgc gcgtgaggaa ggtcttgccc gtcccgctct tgccgaacac
     4921 gccgctcgag cgctcgacga agcgcctgag actaatgtag atcgggatgt cttccatgtc
     4981 gagcggctcg ccgacgtgga agtagccttc gtccttgctg ctgccgaaaa tcaggttcac
     5041 gtcttccgcg ctcgcttcga cgacgggcgc gaaatggccc ggcaccgtct tgaccggctt
     5101 cggctcagtc gcgcccgctt gcgcgtcgag catcagcatg ggcgtgacgt ggatcgcgcc
     5161 gaagacgttg gtgccgatca tcacctcgcg cgtgaagtcg tcgctcaggt cgggcggcag
     5221 cttggcaatg cgcggatcgg tcgcgtccag cgacacgtcg gtgatcatcg agaaaaagcg
     5281 gcgcgaccgg ccttcgacga cgacgtagcg cccgaccgcc gtcgattcga tgtccgccgt
     5341 ccggtcgagc ttcacctcca gacccttgct caacgatccg ccgacgacca cgccgagccg
     5401 ggacgcggcg ggccgatctt cgatctcca
//
LOCUS       DBNHXB010000486         7927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4019603, whole genome shotgun sequence.
ACCESSION   DBNHXB010000486 DBNHXB010000000
VERSION     DBNHXB010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7927
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4019603"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="AC162G_18750"
     CDS             complement(<1..147)
                     /locus_tag="AC162G_18750"
                     /inference="COORDINATES: protein motif:HMM:NF015289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HIR5077901.1"
                     /translation="MHLARVKGTVVATRKYTGLEGVKFLIVQPLDKRQQPDGDPLVAA
                     DATAQ"
     gene            complement(154..453)
                     /locus_tag="AC162G_18755"
     CDS             complement(154..453)
                     /locus_tag="AC162G_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
                     /protein_id="HIR5077902.1"
                     /translation="MSLYRVTFDKALKRQVAGLPGKLRQEAKRRIADLARDPRPPDAV
                     ELKGYAGIYRLWLQDARYRLIWEVFEDETRVEVYYVGIKPDYDGLLKQKTASKPG"
     gene            complement(450..716)
                     /locus_tag="AC162G_18760"
     CDS             complement(450..716)
                     /locus_tag="AC162G_18760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077903.1"
                     /translation="MGKILREKFPIVYEAGKPQAVIVDVETFDRILQALEHLRQLAED
                     PEETRWILEVIEQARAYREAHPEEVWTYDSPEAVMAALDEADSE"
     gene            complement(797..1438)
                     /locus_tag="AC162G_18765"
     CDS             complement(797..1438)
                     /locus_tag="AC162G_18765"
                     /inference="COORDINATES: protein motif:HMM:NF013129.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HIR5077904.1"
                     /translation="MEIAVETVALALVEFGSIAAGMHAGDAMAKKAPIARLVAGTVHP
                     GKYLVLISGAVADVEESLKAGRDAGASHVVDFVFLPQVHPSVVTAIAASAAPGGEGER
                     APRGPSLGIIETSTVAAAIHAADAGVKGAEVSLLELRLADGLHGKGLVLYTGEVPDVE
                     AAVEIGARAIDSSLIVNQIVIPQLHGEMGANIVARTRFGPNVESGWSGGQMVG"
     gene            complement(1464..2552)
                     /locus_tag="AC162G_18770"
     CDS             complement(1464..2552)
                     /locus_tag="AC162G_18770"
                     /inference="COORDINATES: protein motif:HMM:NF018290.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE/TadZ family protein"
                     /protein_id="HIR5077905.1"
                     /translation="MAHILVIDDDFDLLNMLQLMLERGGHTVTTTADGADGLAKAREL
                     QPDLAIVDVMMPGMHGYQVCRALRDDPATASIVVLILTARAQPVDRETALAARADDYM
                     AKPVSPVELIRKVDELLENRKKTGLSPSRILTLMSLRGGVGVSSIAVNVALREQRAGA
                     SACLVDLSPASGHAALQLRLNPKISWGEWLHAPADLNRQAAGKYLLAHESGLHVLAAP
                     FLPPIDEPSPVEPFAEMLRLLRERFQRVVVDAPPVLNGAARAALQLADEVWLVLAPEV
                     ASLQSTVAALRALKTLGVGDDKVALVVNQVMPRASLSRQAIEKALGRPVAAALPYDEA
                     QPAAFGHGAPLLISQPDSLLAAAIREMA"
     gene            complement(2552..3103)
                     /locus_tag="AC162G_18775"
     CDS             complement(2552..3103)
                     /locus_tag="AC162G_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077906.1"
                     /translation="MSPAQVGSRRLAVDFFTHSHRISTQFLVRGRPLIATLNDHTLSY
                     IELDVAYISRIDKPGDLVADYAISILRKENVSFCVVAAQVELALKAVPQAQYLSRRSR
                     PVFCTVPSFEIEGQIDIPGALDLHQMMAIGTERFMPIHNGTATISLLQSVSFTGELIL
                     VNKERIQVFCVSDAPAAAPRGSR"
     gene            complement(3113..4573)
                     /locus_tag="AC162G_18780"
     CDS             complement(3113..4573)
                     /locus_tag="AC162G_18780"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003250405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5077907.1"
                     /translation="MAAEPAIIDETRIQQIVAEAIAELRGEALSKTGLTSPPHSGEGQ
                     GAGGVFPDVDSAVAAASAAFREFRRMPLELRAKLIAAMRRGAEEAAATLAHEAWQETG
                     LGRYEDKIEKNLLNARKTPGTEDLPTAAWSGDHGLTIVEWAPFGVVASITPSTNPTAT
                     VIGNSIGNIAAGNVVVFNAHPSAKRCSASTVRMFNRAIQAAGGPANLITCVAEPTIET
                     AQALMRHRGVRVVLVTGGMAVVREALQTGKRAICAGPGNPPVVVDETADLDVAGKGIV
                     KGASFDNNVICTDEKEVIAVDAIADDLKRSLSAHGAVEISSWQLGRLCKAIFEEDRGP
                     GKPGVMNKKFVGKDASVLLAEIGVSAGKEARLIFADVPREHPLVWSEQLMPIIPLVRV
                     RDAGEAIDLAVQAEHGFRHTAVMYSRHIDHLDRMAREIDCSIFVKNGPSLAGLGYNGE
                     GYCSFSIAGTTGEGMTRPSTFCRPRRCTLVDSFRIV"
     gene            complement(4654..5715)
                     /locus_tag="AC162G_18785"
     CDS             complement(4654..5715)
                     /locus_tag="AC162G_18785"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5077908.1"
                     /translation="MTAKILAAEDAPDEARVLIDFLGAQGYTVIQAADGEEAVIKARV
                     ERPDLLLLDVRMPGLDGFQVCERLKSDPATADIPIFMLTGAGAIDDRVRGLGLGAEDY
                     LTKPYDFEELAARIAARMRNKRSTDALREAQQRILDTFQRYVAPQVVEKLLADPHAAQ
                     LGGVRQTVTILHADFRGYAMLAETIPPERLVDVLNNHLGVAARSILRYQGTLDKFMGS
                     AVMAVFNAPLPQADHALRAVHAAADLQDALLKLQTPNSKLQTPGVRLPCSVGICTGDA
                     VVGNIGAAEIVNYTAIGDVVNLAQRVEADAEDGQIVFNDTTYRRVSDFVECEPLGPRT
                     VRGRSGIVDLFRFAGFKQM"
     gene            complement(5721..>7927)
                     /locus_tag="AC162G_18790"
     CDS             complement(5721..>7927)
                     /locus_tag="AC162G_18790"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5077909.1"
                     /translation="RREAQLRRVREASLAISASLDLERVLAEIVDRCARMLEGASVLI
                     YLMNSSIDLLERVVGSHVLASPLSLPPSERGEPEGGGAAEILTFGEGPAAQAADSGQT
                     IRIDAVAEGSAPLDRFGPRRSVNSLVAVPLAYGGDVLGVLEVGFPGSPVAQPDVELLE
                     LMAPHAAMAIAHARLFERSYEMLNMLQGVNDRTAAVSSVAQAILDARFDLHRMLADAL
                     QRAMSLLGLKAGSILLASPAGSHLHVVVHYRFPQDDAVLAPQQSIRPGQPGLAARAAL
                     ERRTVVVPDVERDLLTKPALEQFRRFGIASVAALPMIAGDSVVGVLQVATGSDKALDA
                     GTLDTLHLIATELAQGATAARQFSRVRSDQEQFTAVISSSGEVVLSLDRQGRLTLANE
                     AAQRAFGFDAQEAVGHPLAKATTNVALNRAVEQAVRTGMRERIGFEVPLADESILFCN
                     LSPIADENAQVMGWVAVMQDVTRFKETERMKSDMILTASHDLRNPVNLTLGALDMLRQ
                     NTANWTDTQREVLDLALIGVRRMEALISDLLDLERVERRVGLNLRECDVVAIAGAVVT
                     ETRMLAQSKQQTLEAHVPERLPPVWGDSQRLYQVVANLVSNAIKYTPAGGQIGLALRR
                     EDDHILLEVSDTGRGIPQEAQARIFERFFRVAGSSSIDPTGTGLGLALVKSIVDQHGG
                     RVWVTSQVGRGSTFRVSLPIWRSPAGADPAGRGASSEQAGMVGAGLAPAPEK"
BASE COUNT         1344 a         2671 c         2552 g         1360 t
ORIGIN      
        1 ctgcgccgtc gcatcggcgg cgacgagtgg atcgccgtcc ggctgctggc gcttgtcgag
       61 cggctggacg atcaggaact tgacgccttc caggccggtg tatttgcgcg tcgcgacgac
      121 ggtgcccttc actctggcga gatgcattcc agttcaaccc ggtttcgatg cagtcttctg
      181 tttcaatagc ccatcatagt ctggcttgat gccgacgtag tacacctcga cgcgtgtttc
      241 gtcctcaaac acttcccaaa tgaggcggta acgagcatcc tgcagccaca gccggtaaat
      301 gcccgcgtag cctttcaact cgaccgcgtc tggcgggcgc ggatcacgcg cgagatccgc
      361 aatacgacgc ttggcttcct gacgcaactt tccaggcagc cctgcaactt gccgcttaag
      421 ggccttgtca aaggtgacac ggtagagact cactcggagt ccgcctcgtc caacgctgcc
      481 atcacggctt cgggagaatc ataggtccac acttcctcag ggtgcgcttc ccgatatgcc
      541 cgcgcctgtt caatgacctc tagtatccag cgtgtctcct caggatcctc cgccagttgc
      601 cggagatgtt ccagagcctg caagatgcga tcaaacgtct caacatcgac aatgacagcc
      661 tgcggcttgc cagcttcgta aactattgga aacttctcgc gcagtatctt tcccattgaa
      721 acacctccat aatgccaagc ggggggatac caactgacaa tctttaccac catcgaccgt
      781 cggagtctga gcctgactac ccgaccatct gaccacctga ccaccccgac tcaacattcg
      841 ggccaaagcg cgtgcgggcg acgatgttcg cgcccatctc gccgtgcagt tgcgggatga
      901 cgatctgatt gacgatcagg ctggaatcga tcgcccgtgc gccaatctcg accgccgcct
      961 ctacatcggg cacttcgccg gtgtacagca cgagtccctt accgtgcaag ccgtcggcga
     1021 gccgcagctc gagcagcgaa acctccgcgc ccttgacgcc cgcatcggcg gcgtgaatcg
     1081 ccgccgccac cgtgctcgtc tcgatgatcc cgagcgacgg gccgcgcggc gcgcgctcgc
     1141 cctcgcctcc cggcgcggcg ctcgccgcaa tggccgtcac gaccgaggga tgcacctgcg
     1201 gcagaaacac gaagtcgacc acgtggctcg cgccggcgtc gcgtcctgcc ttcagcgatt
     1261 cctcgacgtc ggcgaccgcg ccgctgatca agacgaggta ttttcccggg tgcaccgtgc
     1321 cggccaccag gcgcgcgatc ggcgctttct tggccatcgc gtcgccggca tgcatgcctg
     1381 ccgcaatgct gccgaactcg accagtgcga gtgcaaccgt ctcaaccgca atctccaatc
     1441 cctaatctcc aatgaccaaa aggctacgcc atctcgcgta tggcagccgc caggagcgaa
     1501 tcgggctggc tgatcaacag cggggcgccg tgcccgaagg cagccggctg ggcttcgtcg
     1561 tagggcagcg cggccgccac gggccggccc agcgccttct caatcgcctg tcgcgacagg
     1621 ctggcgcgcg gcatcacctg gttgaccacc agggcgactt tgtcgtcgcc gacgccgagg
     1681 gtcttgagcg cgcgcagcgc cgccaccgtc gattgcagcg aggcgacctc tggcgccagc
     1741 accagccaga cctcgtcggc gagctgcagc gccgcgcgcg ccgcgccgtt gagaaccgga
     1801 ggcgcgtcga cgaccacgcg ctggaatctt tcccgcagca gccgcagcat ctcggcaaac
     1861 ggctcgacgg gggatggctc gtcaatcggc ggcaggaacg gcgcagccag cacgtgcagg
     1921 ccggactcgt gcgccagcaa gtacttgccg gccgcttgcc gattcagatc ggccggcgcg
     1981 tgcagccact cgccccacga gatcttgggg ttgagccgaa gctgcagcgc ggcgtgtccc
     2041 gaagccggcg acagatcgac caggcaggcg ctggcgcctg cccgctgctc gcggagcgcc
     2101 acattgacgg cgatggagct taccccgacg ccgccgcgca gcgacatcaa cgtgaggatg
     2161 cgagacggcg acagacccgt ctttttcctg ttctccagca gctcgtcgac tttccggatc
     2221 agctcgaccg gcgagaccgg cttcgccatg tagtcgtcgg ccctggccgc cagcgccgtt
     2281 tcgcggtcga ccggctgggc gcgcgcggtt agaatcagga ccacgatgct ggccgtggcc
     2341 gggtcgtcgc gcagcgcgcg gcagacctga tagccgtgca tgccgggcat catcacgtcg
     2401 acgatagcca ggtcgggctg cagctcgcgc gctttggcca acccgtcggc gccgtcggcc
     2461 gtcgtcgtca ccgtgtggcc gccgcgctcg agcatcaatt gcagcatgtt gagaaggtcg
     2521 aagtcgtcgt cgatgacgag aatgtgggcc attaccggct ccctcgaggg gcagccgccg
     2581 gcgcgtcgct cacgcagaag acttgaatgc gctccttatt caccaggatc agctcgccgg
     2641 tgaagctcac cgactgcagc agcgagatcg tcgccgtgcc gttgtggatc ggcatgaagc
     2701 gttcggtccc gatcgccatc atctggtgca ggtcaagcgc gcccgggatg tcgatctggc
     2761 cttcgatctc gaaggacgga acggtgcaga agacaggccg gctgcgccgc gacagatact
     2821 gcgcctgcgg caccgctttg agcgccagct cgacctgcgc ggcgacgacg caaaacgaga
     2881 cgttctcttt gcgcaggatc gagatggcat agtcggccac cagatcgccc ggcttgtcga
     2941 tgcgagatat ataggcgaca tccagctcga tatacgacaa ggtgtggtcg ttcagcgtgg
     3001 cgatcagcgg ccggccacgc accaggaact gcgtcgagat gcggtggctg tgggtgaaga
     3061 aatctacggc cagccggcgc gagccgactt gtgcaggcga catcggtttt cctcacacga
     3121 tcctgaacga atcgaccagc gtgcagcggc gcgggcggca gaacgtgctg gggcgggtca
     3181 tgccctcgcc cgtcgtgccg gcgatagaga acgagcagta gccttccccg ttgtatccca
     3241 ggccggccag gctcggcccg ttcttgacga agatcgagca gtcgatctcg cgcgccatcc
     3301 gatccagatg atcgatgtgc ctggaataca tcacggccgt gtgccggaat ccgtgctcgg
     3361 cctgcaccgc cagatcgatc gcctcaccgg cgtcccgcac gcgcaccaac gggatgatgg
     3421 gcatcaactg ttcgctccac accaacggat gctcacgcgg gacgtcggcg aagatcaggc
     3481 gagcttcctt cccggcgctg accccgatct cggcgagcag cacgctggcg tctttgccga
     3541 cgaacttctt gttcatcacg cccggtttgc ccgggccgcg gtcttcctcg aagatcgcct
     3601 tgcacaggcg tccgagctgc caggaagaga tctcgaccgc gccgtgcgcc gacaggctgc
     3661 gcttgaggtc gtcggcgatg gcgtccacgg cgatgacctc tttctcgtcg gtgcagatca
     3721 cgttgttgtc gaacgacgcg cccttgacga tgcctttgcc ggcgacgtcg agatcggccg
     3781 tctcgtcgac gacgactggc ggatttcccg gcccggcgca gatggcgcgc ttgccagtct
     3841 gcagcgcttc acgcacgacg gccatgcctc ccgtaaccag cacgacgcgc acgcctctgt
     3901 gacgcatcag cgcctgcgcc gtttcgatcg tcggttcggc gacgcacgtg atcagattgg
     3961 ccggcccgcc ggcggcctgg atcgcgcgat tgaacatgcg aaccgtgctg gccgagcagc
     4021 gcttggccga cgggtgcgcg ttgaagacga cgacgttccc ggcggcgata ttcccgatcg
     4081 agttgccgat cacggtcgcg gtgggattgg tgctgggtgt gatggaagca accaccccga
     4141 agggcgccca ttcgacgatg gtcaggccgt gatcgccgct ccacgcggcc gtgggcagat
     4201 cttccgtgcc cggcgtcttg cgcgcgttaa gcaggttttt ttcgatctta tcttcatacc
     4261 ggcccaggcc ggtttcctgc cacgcctcat gtgccagggt agcggcagcc tcttcggcgc
     4321 cgcgccgcat cgcggcgatc agcttcgcgc gtaattcgag cggcatgcgg cgaaactccc
     4381 ggaaggcggc gctcgcggcg gcgacggcgc tatccacatc gggaaacacg ccccccgccc
     4441 cttgcccctc ccccgaatgc gggggagagg taagccccgt cttggagagg gcctctccgc
     4501 gcagctcggc gatggcctcc gccacgattt gttggatacg cgtctcgtcg atgatggctg
     4561 gctcggcggc cattcctcac ccccttcccc ctcctggtgg gagaggcggg tcaacgtaaa
     4621 gcgtaaaacg tgaagagacc caccccatca gggtcacatc tgcttgaacc cggcgaagcg
     4681 gaacaggtcg acgatgccgg atcggccacg cacggtgcgc gggccgagcg gctcgcactc
     4741 gacaaaatcc gaaacgcggc ggtacgtcgt gtcgttgaac acgatctggc cgtcttccgc
     4801 gtccgcttcg acgcgctgcg ccaggttcac gacgtcgccg atagccgtgt aattgacgat
     4861 ctcggctgcg ccgatgttgc ctactaccgc gtcgccggtg cagattccca cagaacaagg
     4921 cagccgcacc cccggagttt ggagtttgga gtttggagtt tggagtttga gcagcgcgtc
     4981 ctgcagatcg gcggcggcat gcacggcgcg cagcgcatga tccgcctgcg gcagcggcgc
     5041 gttgaagacg gccataaccg cactccccat gaatttgtcc aacgtgccct ggtatcgaag
     5101 aatcgatcgg gccgccacgc caagatgatt gttgagcacg tcgacgaggc gctcgggcgg
     5161 aatcgtctca gcgagcatgg catatccccg aaaatcggcg tgcagaatgg tgaccgtctg
     5221 gcgcaccccg cccaattggg ccgcgtgcgg atcggccaga agtttttcca caacctgcgg
     5281 cgcgacgtag cgctggaacg tgtcgaggat gcgctgctgc gcctcgcgca gggcatcggt
     5341 cgagcgcttg ttgcgcatgc gcgcagcaat gcgcgccgcc agctcttcaa aatcgtaggg
     5401 cttcgtcagg taatcttccg cgcccaggcc cagcccgcgc acgcgatcgt cgatagcgcc
     5461 ggcgccggtc agcatgaaga tcggaatgtc ggcggtagcc ggatcggact tgagccgctc
     5521 gcacacctgg aagccgtcga ggccgggcat gcgcacgtcc aggagcagga gatcgggtcg
     5581 ctcgacgcgc gctttgatga cggcttcctc gccatcggcg gcctggatca cggtatagcc
     5641 ctgcgctccc agaaagtcga tcagcacgcg cgcttcgtcg ggcgcatctt cggcggcaag
     5701 aatcttggcc gtcatcacga tcatttctct ggggcagggg caagccccgc ccctaccatg
     5761 ccggcctgct cgctgcttgc gccccgccca gcggggtcgg cgccggccgg cgagcgccat
     5821 atcggcaggc tgacgcgaaa ggtggagcca cggccgactt gactggtcac ccatacgcgg
     5881 ccgccgtgct ggtcgacgat ggatttgacg agcgccagac ccaaccccgt gccggtcggg
     5941 tcgatcgagg acgatccggc gacgcggaag aatcgctcga agatgcgggc ctgcgcttcc
     6001 tgcgggattc cccgtcccgt gtcactcact tccagcaaga tatgatcgtc ttcccgtcgc
     6061 agcgccagcc caatctggcc gccggcaggc gtgtatttga tggcattact gaccaggttg
     6121 gcgacgacct ggtacagtcg ctgtgaatcg ccccacaccg gcggcaggcg ctccggcacg
     6181 tgagcttcga gcgtctgctg tttggattgc gccagcatgc gcgtctcagt aacgacagcc
     6241 ccggcgatcg cgaccacgtc gcactcgcgc aagttcagcc cgacgcgcct ctcgacgcgc
     6301 tccaggtcga gcaaatccga aatcagcgcc tccatacggc gcacgccgat gagggcgaga
     6361 tcgagcactt cgcgctgggt gtccgtccaa ttggccgtgt tctggcgcaa catgtccagc
     6421 gcgccgagcg tcaaattaac cgggttgcgc aggtcgtgcg aggcggtgag gatcatgtcg
     6481 cttttcatgc gctcggtttc cttgaagcgc gtgacgtctt gcatcaccgc gacccagccc
     6541 atgacctgcg cgttttcgtc ggcaatcggc gagaggttgc agaacaggat ggactcgtcg
     6601 gccagcggca cctcgaatcc gatgcgctcg cgcatcccgg tgcgcacggc ctgctcgacg
     6661 gcgcgattca gcgcgacgtt cgtcgtggct ttggccaaag gatggccgac ggcttcctgg
     6721 gcgtcaaagc cgaaggcgcg ctgcgccgcc tcgttcgcca gcgtgaggcg cccctggcga
     6781 tccaggctga gcacgacctc gccgctgctg ctgatgacgg ctgtgaattg ctcctgatcg
     6841 gagcgcacac ggctgaactg gcgcgcggcg gtagcgccct gggccagctc ggtggcgatg
     6901 agatgaagcg tatcgagcgt gccggcgtcg agcgccttat cgctgccggt ggcgacctgc
     6961 agcaccccga cgacgctatc gccggcgatc atcggcagcg ccgccacgga cgcgataccg
     7021 aaccggcgga attgctcgag cgccggcttc gtgagcaggt cgcgctcgac atcgggcacg
     7081 acgaccgtcc ggcgttccag cgcggcgcgc gcggccaggc cgggctggcc ggggcggatc
     7141 gactgttgcg gagccaggac ggcgtcgtcc tgcgggaacc gatagtgaac gacgacgtgc
     7201 aggtggctgc ccgccgggct ggcgagcaga atcgagccgg ctttaagccc gaggagggac
     7261 atcgcgcgct gaagcgcgtc ggccagcatg cggtgcagat cgaagcgggc gtccaggata
     7321 gcctgggcga cgctgctgac cgcggcggtg cgatcgttga cgccctgcag catgttgagc
     7381 atctcgtagc tgcgctcgaa gagccgggcg tgtgcgatag ccatggcggc gtgcggggcc
     7441 atcagctcca gcagctcgac gtccggctgc gcgaccgggc tgccggggaa gccgacttcg
     7501 agcacgccaa gcacgtcgcc gccataagcc aggggcacgg cgaccaggct gttgacgctg
     7561 cgcctcggcc caaaccggtc gagcggcgcg ctcccctccg cgacggcgtc gatccggatc
     7621 gtttggccgg agtcggcagc ctgcgcggcc ggcccctcgc caaaggtgag gatttcggcc
     7681 gcgcctcccc cctccggctc cccccgttct gacgggggga gggagagggg ggaagccagc
     7741 acatggctgc cgacgacccg ctccagcagg tcgatgctgc tgttcatcag gtagatcagg
     7801 acagacgcgc cttccagcat ccgcgcgcag cgatccacaa tctcggccag aacgcgctcg
     7861 aggtcgagcg aagccgagat cgccagcgac gcctcgcgca cccggcgcaa ctgcgcctcg
     7921 cgccgct
//
LOCUS       DBNHXB010000487        10229 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_833400, whole genome shotgun sequence.
ACCESSION   DBNHXB010000487 DBNHXB010000000
VERSION     DBNHXB010000487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10229)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10229)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10229
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_833400"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="AC162G_18795"
     CDS             <1..469
                     /locus_tag="AC162G_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077910.1"
                     /translation="DYLERDEDIVVPVKKMWNEWHAGHPAPPLEAFTAIVRADDRIEE
                     MGEVDHTEDMDWMSPEELDEYVRDMEADGYYSGPRVKLKSRELTLEHIARMIKRHNDR
                     MEEALRQAHAALPDDVSEEEEGQLIDVMALAEDLRRRLREAGLEADDERGAAP"
     gene            complement(486..1034)
                     /locus_tag="AC162G_18800"
     CDS             complement(486..1034)
                     /locus_tag="AC162G_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077911.1"
                     /translation="MPETDRPKRARRLYDWEIEEAKLVFADRLNTDRVRVHECAEWPD
                     TIHHLGKRLRGLPPPAKGEHNAMTLGNNCYFPLPMPERPTSPDDPFGMGWLIHELTHA
                     WQFQTIGWLYLIRALLAQLTSRPYEFGGEAGLRAMREKNGTLLSFNPEQQGAITSTYY
                     MRLKREHDVSAWEPYIQDIRAA"
     gene            complement(1056..1886)
                     /locus_tag="AC162G_18805"
     CDS             complement(1056..1886)
                     /locus_tag="AC162G_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5077912.1"
                     /translation="MKLAVLADIHANLPALLAVSAHIDAWQPHRVIVAGDVVNRGPRP
                     AECLRFVQDRQRERDWQVVIGNHEEYVIHQAHPDAPRSGPQFDIHIGSYWTYRKLGED
                     VSALEAMPFQLSLPGPDGSDVRVTHASMAGTRVGIFPRTTDSELGMLIGSPAPALFCI
                     GHTHIPFVRSLDGVLVVNVGAVGLPFDGDTRAGYAQIVWSHDRWQAGIVRLDYDRAQA
                     ERDFYDTGFLDEAASFARLVHVELRTARSLIYDWTRRYEARVLEGELTVEEAVEDYLN
                     "
     gene            2038..4563
                     /gene="pbpC"
                     /locus_tag="AC162G_18810"
     CDS             2038..4563
                     /gene="pbpC"
                     /locus_tag="AC162G_18810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HIR5077913.1"
                     /translation="MVRRSSLVIGRWKLILLVCAALSLVCAGGLYAWLFVDLPSLDTL
                     YERAAAPSTKILDRRGRLLVEIIDPHRGKHTPVPLDAIPLDCRQATIATEDANFYTNP
                     GVDLAGILRAMWINLQGGETLSGGSTITQQLARNLLLSPEERAQRTLTRKLRESILAW
                     RMAQRFSKDEILALYLNEVYYGNLAYGIEAAAQAYFGAPVGQLDLAQCALLAGLPQSP
                     ANYDPLVNLPAAKERQSVVLDLMVKAGTVDAEQAGLAKAENLRFAATPFPIEAPHFVA
                     YVTAWLEDRFGLEAIHTQGLIVTTTLDLDWQRAAEAIARRRLKELQDGTALSIPEGQR
                     GSALRVNNAALVALDPHTGEILTMLGSPGYFEAQIDGAVNAAIALRQPGSSIKPITYA
                     AAFDPAAPDPMTAATLILDVRSSFPTREGDPYVPRNYDLRFHGPVSAREALASSYNIP
                     AVKVLQRIGLDRMIALARNLSIGTFGDSSRYGLSLTLGGGEVRLIDMAAAYGAFANGG
                     RRVIPASVLHVTAATGDVLYKVQPQLGEQVLDPRVAYLVSDILSDNNARAPAFGEFSV
                     LRLTRPAAVKTGTTTDWRDNWTVGYTPDLVVGVWVGNADNQPMERVSGVAGAGPIWHD
                     FIETALKNAPASDFERPEGLVDLDVCATSGLLPTPYCPFTKREVFIAGTEPAQPDNLY
                     RPFEIDVATGQLATPDTPPERVATRVYLVLPAEAQEWARENGIPQFLQFPQFQAGKGT
                     QGTEGTEMVRITRPDDGTIYRITPQTPIGSQRIPVQAIAADGVQIAQLTLFVDGEPIG
                     TFNHTPARTWWTLSPGRHVFTVEAIDGQGNVLAGAPVTIIVTQ"
     gene            4681..5439
                     /locus_tag="AC162G_18815"
     CDS             4681..5439
                     /locus_tag="AC162G_18815"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5077914.1"
                     /translation="MRLKGILFDLGSTLLEFRGEWNEVMARGAQEQVDFFLARGLQLD
                     REAFLKRHRELIMMFIEKGQQDWIEYSSDKALTMALAEFGYPDLPQSLIDESLKMLYA
                     YGETLWRPFPDTYATLDALRDRGYKMAIISNARDASNVHRLIDTHHLRPWFDPILVSS
                     EFGVRKPNPRIFHAVLDEWGIRPGEAVMVGDMLGADVLGAKNAGMRSVWATMQAARSA
                     NETHLDMIVPDATIASLSELLPLLESWEAGRLVP"
     gene            5573..7045
                     /locus_tag="AC162G_18820"
     CDS             5573..7045
                     /locus_tag="AC162G_18820"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5077915.1"
                     /translation="MSLRSRLLLSYVLVVLAFLTVIVLGATILLRDNPVQTQVLTREL
                     AGAARLVARSSRLAVQSGASPEQIIRRLSNAAPGDIRLMFVDGASGTVLADSGGALSG
                     VNLYELSGGRPAGLTLEGEFESGGKRWLYAAQPVEIGRRSGTLVVAAAPAPSTPLLRD
                     PTFLGLLRPLLIAAAIALVIASILAVIVARSIAWPIQHVASAANAIASGDLAQKAPVE
                     GPHEVRELATSFNAMADRVRASQQSQRDFLANVSHELKTPLTSIRGFAQAIADGAAGD
                     GESLRKSASIIREESERMARMVGDLLDLARIETGQLAMLRLPLQVGDVLRSCIERQSL
                     RAQSASVSLALDTPGDLPVIHGDGDRLAQVFNNLIDNALKHTPPGGKVAVSARAMTGS
                     SVARRGKPWPGAVEVSVADSGPGIPAEDLSRIFERFYQVEKSRARSASGGSLGLGLAI
                     AREIVTAHGGSIHAESVTGLGSKFVVRLPTEPAMPLPSTP"
     gene            7099..7482
                     /locus_tag="AC162G_18825"
     CDS             7099..7482
                     /locus_tag="AC162G_18825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077916.1"
                     /translation="MRVGAEILFPPYAIPYLLDLRGPKWRQLIEHVLRLKEDHPEALA
                     FSWMMMRLDGCRTCEVDSFKAMQGCVACAVQTVRRFKGSDAELLKLYDEALREVQAYL
                     SERRKAERRAKPIARVAAGVPLVRG"
     regulatory      7534..7691
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            7837..>10229
                     /locus_tag="AC162G_18830"
     CDS             7837..>10229
                     /locus_tag="AC162G_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR5077917.1"
                     /translation="MHPHTQRRYLDALADHVVVFDGAMGTSIQACRLGPADFGGESLN
                     GCNDYLVISRPEVIEGIHASFLAVGCEAIETDTFRANRITMREYGLQDRIVEINRAAA
                     VLARHVADRFEAETGVPRYVAGSIGPSGMLPSSGDPALGRITFQELVDVFREQSLGLI
                     QGGCDLLLIETQQDILETKAAVHGAWRAFEETGVRIPLQVQITLDTTGRMLLGTDIAA
                     ALTTLECLPVVDVIGLNCSTGPDYMREPVRYLAAHARKPISVIPNAGLPLNVDGDAVY
                     PMKPVPLAETLAEFVTELGVNAVGGCCGTTPEHLKEVIDRVRGLKPKKTLTAESAMDA
                     ASFQSISAPSAVSGAPMISSAMRAIPLRQDPPPMIVGERVNSQGSRKVKRLLLADDYD
                     GILEVAREQVEGGAHALDVCVALTERADEADQMRRVVKKLQMSVEAPLMIDSTEADVV
                     QVALETNPGRSIINSINLENGRKRCDAVLPLAVAHGAAVIALTIDERGMAKTASAKLD
                     VAKRIYDIACDEYGLPADALIFDTLTFTLATGDPEMAGSAVETIEGIRMIKAELPGVL
                     CSLGVSNVSFGLAPAARAALNSVFLYHCVRAGLDMALVHPKDITPYIEIDADVRALCD
                     DLIFNRTLDALAKLIHHFESAGVAEAGKAVEDPTEGMTPEQKIHWMILHRKRDGIEPL
                     IDASIVAHGLAESKTPNDGAIWTLNNVLLPAMKEVGDRFGAGELILPFVLQSAEVMKK
                     AVAHLEQYLDRKEGATKGKVVLATVYGDVHDIGKSLVNTILTNNGFTVFDLGKQVPA"
BASE COUNT         1841 a         3228 c         3356 g         1804 t
ORIGIN      
        1 ggactatctc gaacgcgatg aagacatcgt ggtgccggtc aagaagatgt ggaacgagtg
       61 gcacgccggg catccggcgc cgccgctgga ggccttcacg gccatcgtgc gggccgacga
      121 ccgcatcgaa gagatgggcg aggtcgatca caccgaagat atggactgga tgagtccgga
      181 agagctggac gagtacgtgc gggacatgga agcggacggc tattattccg gcccacgcgt
      241 gaagctcaaa tcgcgcgagc tgacgctgga gcacatcgcc cgcatgatca agcggcacaa
      301 cgaccggatg gaagaggcgc tgcgtcaggc ccacgcggcg ctgcccgacg acgtgagcga
      361 ggaggaagaa ggccagctca tcgacgtgat ggctttggcg gaagacctcc gccgccgttt
      421 gcgtgaagcc ggcctggagg ctgacgacga gcggggcgct gctccctgac ggtgcgctcc
      481 ggaggtcacg cggctcgaat gtcttgaatg taaggctccc aggcgctgac gtcgtgctct
      541 ctcttcaggc gcatgtagta cgtgctggtg atcgcccctt gttgctccgg attgaagctg
      601 agcagcgttc cgttcttttc gcgcatggcg cgcagtcccg cttcgccgcc gaattcataa
      661 ggccgcgacg tcagttgagc cagtaaggcc cggatcaagt acagccaacc gatcgtctga
      721 aactgccagg cgtgcgtcag ctcgtgaatc agccagccca tgccgaaggg atcgtcgggg
      781 gacgtgggcc gttcgggcat cggcagggga aagtaacagt tgttgcccag ggtcatggcg
      841 ttgtgttcgc ctttggcggg aggtggcagg ccgcgcagac gcttgcccag gtggtgaatc
      901 gtgtcgggcc attcggcgca ctcgtgcacg cgcacgcgat cggtgttcag gcggtcggcg
      961 aaaacgagct tcgcttcctc gatttcccaa tcatacagcc gccgggcacg ttttgggcgg
     1021 tccgtctcag gcatcggtgt tctcccttct caccatcagt tgagataatc ctcgacggct
     1081 tcctcaacgg tcagctcgcc ctccagcacg cgagcctcgt agcggcgcgt ccagtcgtag
     1141 atcaacgagc gcgccgtgcg cagctcgacg tgcaccagtc gcgcgaacga cgccgcttcg
     1201 tccagaaagc ctgtatcgta gaaatcgcgc tcggcctgcg cccggtcgta gtcgaggcgg
     1261 acgatgccgg cctgccatcg atcgtgtgac cagacgatct gcgcatagcc ggcgcgcgtg
     1321 tcgccgtcga agggcagtcc caccgcgccg acgttgacga cgagcacgcc gtcgagcgag
     1381 cgcacgaatg ggatatgtgt gtgcccgata caaaataacg ccggagccgg gctcccgatc
     1441 agcatgccca gctcgctatc cgtcgtgcgc gggaaaattc ctacgcgcgt gcctgccatc
     1501 gacgcgtgcg tcacgcgaac gtcgctcccg tccgggccgg gaaggctgag ctggaaaggc
     1561 atcgcctcca gcgccgacac atcttcaccg agtttccggt acgtccagta cgagccgatg
     1621 tgaatatcga actgagggcc gctgcgcggc gcgtcggggt gggcctggtg gatgacgtat
     1681 tcctcgtggt tgccgatcac gacctgccag tctcgctcgc gctgcctgtc ttggacgaag
     1741 cgaaggcact cggccgggcg cggcccgcga ttgacgacgt cgccggcgac gatcacgcga
     1801 tgcggctgcc aggcgtcgat gtgcgcggac acggcgagca gcgcaggcag attagcgtga
     1861 atgtcggcga ggacggcgag tttcatagtg ttgggtaatt agtaatcagt aatcagtgag
     1921 cagtgacctc tgaccactgg caaccggcca ctattataac gcacccttga ggcttgcccg
     1981 ccgctttctc tgcgctacaa tgtgcaacaa tccgaagcgc atgggcgtct gataagcatg
     2041 gttcgccgtt cttcactcgt cattggtcgt tggaagctta ttctactggt ctgcgccgca
     2101 ctctcgctcg tgtgcgcggg tggcctctat gcctggctgt tcgtcgatct gccgtcgctc
     2161 gatactctgt acgagcgagc tgccgcgcca tcgacgaaga ttctcgaccg gcgcgggcgg
     2221 ctgctggtcg agatcatcga tccgcatcgc ggcaagcaca cgccggtgcc gctcgacgcg
     2281 atcccgctcg attgccgcca ggcgacgatt gcgaccgagg atgccaactt ttacactaac
     2341 ccgggcgtcg acctggccgg catcctgcgc gcgatgtgga tcaatttgca ggggggcgag
     2401 acgctgtcgg gcggcagcac gatcacgcag cagctcgcgc gcaacctgct cctctcgcca
     2461 gaagagcgcg ctcagcgcac gctaacgcgc aaacttcgcg aatcgattct cgcctggcgc
     2521 atggcgcagc ggttcagcaa ggacgagatc ctcgcgctgt atctcaacga ggtgtattat
     2581 ggcaatctgg cctacggcat cgaagcggcg gcgcaggcat actttggggc gccggtcggc
     2641 cagctcgatc tggcgcagtg cgcgctgctc gccggactgc cgcagtcgcc tgcaaattac
     2701 gatccgctcg tcaatcttcc tgccgcgaag gagcggcagt cggtggtgct cgacctgatg
     2761 gtgaaagcgg gcaccgtcga tgccgagcag gccgggctgg cgaaggcgga gaacctgcgc
     2821 ttcgccgcga cgccgtttcc gatcgaagcg ccgcacttcg tcgcctacgt gaccgcgtgg
     2881 ctcgaagacc gcttcggcct cgaggcgatt cacacgcagg ggttgatcgt cacgacgacg
     2941 ctcgacctcg actggcagcg cgccgccgaa gcgatcgcgc ggcggcgctt gaaggagctg
     3001 caggatggca cggcactgag catccccgaa ggccagcggg gcagcgcttt gcgcgtcaat
     3061 aatgccgcgc tcgtcgcgct cgatccgcat acgggcgaga tcctgacgat gctcggcagc
     3121 ccgggctatt tcgaggcaca gatcgacggc gcagtcaatg cggcgatcgc gctccggcag
     3181 cccggatcgt cgatcaagcc cattacctac gcggcggctt tcgatccggc tgcgcccgat
     3241 ccgatgacgg cggccaccct gatcctcgac gtgcgatcgt ccttccccac gcgcgaaggc
     3301 gatccgtacg tcccgcgcaa ttacgatttg cgattccacg ggcctgtttc ggcgcgcgag
     3361 gcgctggcgt cgagctacaa catcccggcg gtgaaagtgc tccagcgcat cggcctggat
     3421 cggatgatcg cgctcgcgcg caatttgagc attggcacgt tcggcgattc gagccgctac
     3481 gggctgtcgc tcacgctcgg cggtggtgaa gtccgcctga tcgacatggc cgccgcgtac
     3541 ggcgcattcg ccaacggcgg gcggcgcgtg attccggcat ccgtgctgca cgtgacggct
     3601 gcaacgggcg atgtccttta caaggtgcag ccgcagctcg gcgagcaggt actcgacccg
     3661 cgcgtggcat acctcgtcag cgacatcctg agcgacaaca atgcgcgcgc gcccgcgttc
     3721 ggcgagttca gcgtgctgcg cttgacgcgt cccgccgccg tcaagactgg cacgacgacc
     3781 gattggcgcg acaactggac ggtgggctac acgcccgacc tggtcgtcgg cgtgtgggtt
     3841 ggcaacgccg acaaccagcc gatggagcgc gtgagcggcg tcgcgggcgc cggcccgatc
     3901 tggcacgact tcatagagac ggcgctcaag aatgcgccgg cgagcgattt cgagcgaccc
     3961 gagggactgg tcgatctcga cgtgtgcgcg acctccggcc tgctgccgac gccatattgc
     4021 cccttcacga agcgcgaagt gttcatcgcc ggaaccgagc cggcgcagcc cgacaacctg
     4081 tatcggccgt ttgaaatcga cgtggcgacg ggccagcttg ccacgccaga tacgccgccc
     4141 gagcgcgtcg cgacgcgcgt gtatctcgtc cttcccgccg aggcgcagga gtgggcacgt
     4201 gagaacggga tcccgcagtt ccttcagttc cctcagttcc aagctggtaa gggaactcaa
     4261 ggaactgaag gaactgagat ggttcgtatc acgcggcccg acgacggaac aatctataga
     4321 atcacgcctc agacgccgat cggctcgcag cgcatcccgg tgcaggctat cgccgccgac
     4381 ggcgtgcaaa ttgcgcagtt gacgctgttt gtggatggag agccgatcgg gacgttcaat
     4441 cacacgccgg cgaggacgtg gtggacgttg tcgcccggca ggcacgtgtt cacggtcgag
     4501 gcgatcgacg ggcaggggaa cgtgctggcc ggcgcgccgg tgacgattat agtgacgcaa
     4561 tgatctgagc gcttacccga ccacgatgat gcggccgttt gacgagattt cacatcgcgt
     4621 ttacagcctg tttacaacaa tgcttcagac agatggaacg tctcgtgtat aatcttcctc
     4681 atgaggctca aaggcatcct cttcgacctc ggcagcacgc tgctcgaatt ccgcggcgag
     4741 tggaacgagg tgatggcgcg cggcgcacag gagcaggtcg atttctttct ggcgcgcggg
     4801 ctgcagctcg atcgggaggc cttcctcaag cggcatcgcg agctgatcat gatgttcatc
     4861 gagaagggcc agcaggactg gatcgaatac agctccgaca aggcgctgac gatggcgtta
     4921 gccgagttcg gctatcccga tctcccgcaa tcgctgatcg acgaatctct caaaatgctc
     4981 tacgcctacg gcgagacgct gtggcggccc tttcccgaca cgtacgccac gctcgacgcg
     5041 ctgcgcgaca gaggctacaa gatggcgatc atttccaatg cgcgcgacgc aagcaatgtc
     5101 catcggctca tcgacactca ccatctacgc ccatggttcg acccgatcct cgtttcttcc
     5161 gagtttggcg tgcgcaagcc gaatccgcgc atcttccacg cagtcctgga cgaatggggc
     5221 ataaggcccg gcgaggccgt gatggtcggc gacatgctcg gcgcggacgt gctcggcgcg
     5281 aagaacgccg gcatgcgcag cgtgtgggcg acgatgcaag ctgcgcgcag tgcgaatgaa
     5341 acacacctcg acatgatcgt gccggacgca acaatcgctt ccctatcgga actgctgccg
     5401 ctgctagaaa gttgggaggc cggccggttg gttccctgac tggagtttgg tacctcgctg
     5461 gagttgatcc tttaggcgga catacgtggc gctcaagacc ggctaaagcc tcgactccgg
     5521 tcctggtctg gcactcgcca aacgtaatca cgcaatcacg catcactctc ccatgtctct
     5581 ccgctcccgc ctgcttctct cctacgttct cgtcgtcctt gcgttcctca cggtgatcgt
     5641 tttaggggcg acgatcctat tgcgagacaa cccggttcag acgcaagtcc tcacgcgaga
     5701 gctggctggc gcggcccggc tcgtcgcgcg ctccagccgg ctggctgtcc aatccggcgc
     5761 gtcgcccgag cagatcatcc ggcggctgag caacgccgcg cctggcgaca ttcgcctgat
     5821 gtttgtcgac ggcgcatcgg gaaccgtcct agccgattct gggggcgcgc tctcgggcgt
     5881 aaacctgtac gagctgagcg gcgggcggcc ggccggcctg acgctggagg gcgagttcga
     5941 gtcgggcgga aagcgctggc tgtacgccgc ccagccggtc gagatcgggc ggcgcagcgg
     6001 gacgctcgtc gtcgccgccg cgcccgcgcc ttcgacgccg ctccttcgtg accccacctt
     6061 tctgggcctg ctcaggccgc tgctcatcgc cgctgcaatc gcgctcgtca tcgcctcgat
     6121 cctggccgtg atcgtcgccc gctcgatcgc ctggcccatc cagcacgtcg cttcggcggc
     6181 gaacgcgatc gcgtccggcg acctggcgca aaaggcgccg gtcgaaggcc cgcacgaggt
     6241 gcgggagctg gcgacgagtt ttaacgcgat ggccgaccgc gtgcgcgcct cgcagcaaag
     6301 ccagcgcgac ttcctggcga acgtatcgca cgagctgaag acgccgctga cgagcattcg
     6361 tggcttcgcg caggcgatcg ccgacggcgc agcgggcgat ggcgaatcgc tccgcaagag
     6421 cgcgtcgatc atccgcgagg aatcggagcg catggcgcgc atggtcggcg atctgctcga
     6481 cctggcgcgc atcgagaccg gccagttggc catgctgcgc ctgccgcttc aggtgggcga
     6541 cgtgctgcgc agttgcatcg agaggcagtc gctccgggcg cagagcgcga gcgtcagcct
     6601 ggcgctcgac acgccgggcg acctgccggt cattcacggc gacggcgacc gcctggccca
     6661 ggtgttcaac aacctgatcg acaacgcctt gaagcataca ccgccgggag ggaaggtcgc
     6721 cgtatcggcg cgggcgatga ccgggtcgtc ggtggcacgc agaggcaagc cgtggccggg
     6781 cgcggtcgag gtgagcgtgg cggacagcgg gccgggcatc ccggccgagg acttgtcgcg
     6841 catcttcgag cgtttctacc aggttgagaa atcccgcgcc cgatctgcgt cgggtggcag
     6901 cctgggattg ggattggcta tcgccaggga gatcgtgacc gcgcacggcg gatcgatcca
     6961 cgcggagagc gtcaccggcc tgggcagcaa gttcgtcgtg cggctgccca ccgagccggc
     7021 catgcccctt ccgagtaccc cttgacgcgc cttttgcacg cgatataatt ctgtcagtcc
     7081 ggacgaaagc gagtcaaaat gagagtcggc gccgagattc tctttccgcc ttatgcaata
     7141 ccgtatcttt tagacctgcg cggcccgaag tggcggcagc tcatcgagca cgtcctacgg
     7201 ctgaaggaag accatcccga ggcgcttgcc ttttcgtgga tgatgatgcg gctggacggc
     7261 tgccgcacct gtgaggtcga ttcgttcaag gcgatgcaag gctgcgttgc gtgcgccgtg
     7321 cagaccgttc gccgtttcaa gggaagtgac gccgagctgc tcaagttgta cgacgaagcg
     7381 ttgagggagg tgcaggccta cctgtccgag cggcggaagg cggaacgacg cgccaagccg
     7441 atcgccaggg tggccgccgg cgtgcccctc gtgcgaggtt gacacctgtc caggttgtgg
     7501 taaactgcgg gcctcaaact gaatagcaca ctgcgctcat ccagagaggc ggagggaccg
     7561 gccctgtgaa gcctcggcaa ccagtctggg gttagagtgc ggagttagag tctggacgct
     7621 aactctcaac tctaactcta actccaagca aggtgccaat tccggcagaa gcaaattctg
     7681 ggagatgaga gcaagcgctc gcatgccgac ttgcctcttc aacccgaagg ggctttttgt
     7741 tgccgtttag agattggaga ttagaggtta gaggttggaa gttggaggca ttagtctcac
     7801 agcctcatac gtcgaaagcg gagagcaacc ttgaaagtgc accctcacac tcagcggcgc
     7861 tacctcgatg cgcttgccga tcacgtcgtc gtgttcgacg gcgcgatggg cacgagcatc
     7921 caggcgtgcc gtctcggccc ggccgacttt ggcggcgagt cgctcaacgg ctgcaacgat
     7981 tacctggtga tcagcagacc tgaagtgatc gaaggcatcc acgcgtcttt cctggccgtc
     8041 ggctgcgagg cgatcgagac tgacaccttc cgcgcgaacc ggatcacgat gcgcgagtac
     8101 ggtctgcagg atcgcatcgt cgagatcaac cgggcggcgg cagtgctggc gcgccatgtc
     8161 gccgatcgct tcgaggcgga aacgggcgtg ccgcgttacg ttgccggctc gattggcccg
     8221 tcgggcatgc tgccctcgtc gggcgatccg gcgctgggcc gcatcacgtt ccaggagctg
     8281 gtcgacgtgt tccgcgagca gtcgctcggg ctgattcagg gcggctgcga cttgctgctg
     8341 atcgagacgc agcaggacat ccttgaaacg aaggccgccg tgcacggcgc gtggcgcgcg
     8401 ttcgaggaaa ccggcgtccg catccccttg caggtgcaga tcacgctcga cacgacaggc
     8461 cgcatgctgc tcggcaccga catcgccgcc gcgttgacga cgctcgaatg cctgcccgtc
     8521 gtggatgtga tcggcctaaa ctgctcgacc ggcccggact acatgcgcga gccggtgcgc
     8581 tacctggcgg cgcacgcgcg caagccgatc tcggtcatcc ccaacgccgg cttgccgctc
     8641 aacgtcgacg gggacgccgt gtatcccatg aaacccgtgc cgctggccga gacgctggcc
     8701 gagttcgtga cggaattggg cgtgaacgcg gttggaggct gctgcggcac gacacccgag
     8761 cacctcaagg aagtcatcga tcgcgtgcgc ggcctcaagc cgaagaagac attaaccgcg
     8821 gagagcgcca tggacgcagc gagcttccaa agcatctctg cgccctctgc ggtgagcggt
     8881 gcgccgatga tctcgtcggc gatgcgcgcg attccgctgc gccaggaccc gccgccgatg
     8941 atcgtcggcg agcgcgtcaa ctcacagggc agtcgcaaag tcaagcgcct gcttctagcc
     9001 gacgactacg acggtatcct cgaggttgcg cgcgagcagg tcgagggcgg cgcgcacgcg
     9061 ctcgacgtgt gtgtggcctt gaccgagcgc gcggacgaag ccgaccagat gcgccgcgtc
     9121 gtcaagaagc tgcaaatgag cgtcgaggcg cccttgatga tcgactcgac cgaggccgac
     9181 gtcgtccagg tcgcgctgga gacgaatccg ggacgttcca tcatcaactc gatcaacctc
     9241 gaaaacggcc gcaagcggtg tgacgctgtc ctgccgctcg ccgtcgcgca cggcgcggct
     9301 gtcatcgcgc tgacgatcga cgagcgcggc atggccaaga ccgccagcgc caagctcgac
     9361 gtggcgaagc gcatctacga catcgcctgc gacgagtacg gcctgcccgc cgacgcgctg
     9421 atcttcgata cactgacgtt cacgctggcg accggcgatc cagagatggc cggatcggcg
     9481 gtcgagacga tcgagggcat ccgcatgatc aaggccgagc tgcctggcgt gctatgctcg
     9541 ctcggcgtga gcaatgtgtc gttcgggctt gcgcctgccg cgcgcgccgc gctgaacagc
     9601 gtgttcctgt accattgcgt gcgggccggt ctcgacatgg cgctcgtgca cccgaaggat
     9661 atcacgccct acatcgagat cgatgctgac gtgcgggcgt tgtgcgacga tctgatcttc
     9721 aaccgcacac tcgacgcgct ggcgaagctg attcatcact ttgagagcgc cggcgtggcc
     9781 gaggcgggca aggctgtcga agatccgacc gaaggcatga cgccggagca gaagatccac
     9841 tggatgatcc tgcatcgcaa gagggacggt atcgagccgc tcatcgacgc gtcgattgtg
     9901 gcgcacggcc ttgccgaaag caagacaccc aacgacggcg cgatctggac gctgaacaat
     9961 gttctactgc cggcgatgaa agaggtcggc gaccgtttcg gcgccggcga gctgatcctg
    10021 ccgttcgtcc tgcaatcggc ggaggtgatg aagaaggcgg tcgcccatct cgagcagtat
    10081 ctcgacagga aagagggcgc gacgaagggc aaggtcgtgc tggccacggt gtacggcgat
    10141 gtgcacgaca tcggcaagag cctcgtcaac acgatcctga ctaacaacgg cttcaccgtg
    10201 ttcgacctgg gcaagcaggt gccggccaa
//
LOCUS       DBNHXB010000488        15809 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_2512225, whole genome shotgun sequence.
ACCESSION   DBNHXB010000488 DBNHXB010000000
VERSION     DBNHXB010000488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 15809)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15809)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15809
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_2512225"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1327)
                     /locus_tag="AC162G_18835"
     CDS             complement(101..1327)
                     /locus_tag="AC162G_18835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5077918.1"
                     /translation="MSEPIRLLHFADIHVGIENYGHIDSATGVNSRVLDFLRRVDEVI
                     DYGLARDVDVVIFAGDAFKTRDPSPTLQREFARRVKRVVDAGVPMILLVGNHDLPAME
                     KKASSIDIYRTLAVPNVVVGWEEALHRVETKRGPIQIATAPYPMRNRLMSLDEHRGKP
                     VDELDRALQEIVGDNVRALAEQLDPALPAVLTGHFTVSGATYGSERSVMLGRDVAVLK
                     SVLADARWDYVALGHIHKHQSLQPKGDYPAIVYSGSLERIDFGEEKEAKGFCWVEVDK
                     GETTWQFVEVKARPFKTIVADVREADDVTMAAQAELAQHDVTNAIVRVIVRMRPEQEA
                     SLRERDVRAVLKAASYVAAIQKDVERVERARLGGHSPEGMTPMELLERYLRSKDMDEE
                     RIRVVLEHARRIIEHE"
     gene            complement(1337..2530)
                     /locus_tag="AC162G_18840"
     CDS             complement(1337..2530)
                     /locus_tag="AC162G_18840"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purple acid phosphatase family protein"
                     /protein_id="HIR5077919.1"
                     /translation="MTGVRKIVSLATRRSWIALLVIAALLAPAVAAQGPLLTRGPYLQ
                     STTRDSTIVVWQTGTAGDSVVEYGEMGYTRVVSSTAPATTHVVSLTGLSAGTTYQYRI
                     GTGNTLLYTSTLATAPDPGGAFDFVMVGDSGAGTNGIPNQELLDVAAQMDALDPDFIL
                     HLGDVIYPKGEASGYQPYFFDPYQNLLDSAPIFPSLGNHDYGTPDAQPYLDVFYLPAN
                     NPLGTERYYSFDWGDAHFVALDTTPPRYEDSAMLQWLQSDLAASTAKWKFVYFHHAMY
                     TSGPHRLDPTLPDMQNTLEPIFSQRDVDIVFNGHDHDYERSRPINGVIYIVSGGGGAN
                     IYPLLPPLQPFSAYAESVHHTVQVQIDGCILSLWAINKDGVVIDRIALAKCPNNVYLP
                     LILKQ"
     gene            complement(2577..3359)
                     /locus_tag="AC162G_18845"
     CDS             complement(2577..3359)
                     /locus_tag="AC162G_18845"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5077920.1"
                     /translation="MPSIDAFRLDGRVAIVTGASSGFGAAIGRLFAQAGARLVLAARR
                     EHHLKEVASSLDAPSGCVLTVQTDVTEEAQVRRVVAMTLECFGRIDVLVNSAGIIART
                     PVPEIEEADWDRMMAVNLKGPFLCCKHVLPHMIAQRDGRIVTIASYLGLFAGSGSTPA
                     YNASKGGVVMLTRSIAVKHGPEGVRANAICPGFVKTELNRDIIDHAPDPEAKEREIAS
                     VYPLRRLGTPDDVACAALYLASDASNWVTGACLMLDGGLTVG"
     gene            complement(3365..3718)
                     /locus_tag="AC162G_18850"
     CDS             complement(3365..3718)
                     /locus_tag="AC162G_18850"
                     /inference="COORDINATES: protein motif:HMM:NF018508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIR5077921.1"
                     /translation="MRNDRRSPSFWLALSRQMRLAWRLFWDRRVPFTNKLLPLATVAY
                     ILSPVDFLPDVILGVGQLDDLGLFLLGVQLFVMICPRGIVEAILEEMEGSVIDGKWRR
                     TDRNPPNPPQLPKSD"
     gene            complement(3718..4071)
                     /locus_tag="AC162G_18855"
     CDS             complement(3718..4071)
                     /locus_tag="AC162G_18855"
                     /inference="COORDINATES: protein motif:HMM:NF018508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIR5077922.1"
                     /translation="MSKRSTPRTSIWVAAKRQARLIFELMRDSRVPIWIRLIPLVGIV
                     YLLSPIELMPEIALLPFGLIDDLVVIIICLTIFLALAPKDVVDDHRGWLDAADITIDD
                     LQETRRLPPNGEEKD"
     gene            4131..5786
                     /locus_tag="AC162G_18860"
     CDS             4131..5786
                     /locus_tag="AC162G_18860"
                     /inference="COORDINATES: protein motif:HMM:NF025011.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HIR5077923.1"
                     /translation="MHLIVYNPGMRSLRQFLIDTEPALLRVIASRWDINLPAGSKTRD
                     VAAVVASWLGDPSHTVAIVERLTPSEREGLRVLIAGGGSLGASAFAQRFGIIRPVGPA
                     RLERDQPWRAPISPAEGLWYLGLIFRAFEQRSGAMQDVMVAPAELHALAALLAAPALP
                     ADTLPPVTAPASVSSSAAQLADDLCTLLAHLLNPHERSWTRQLRDPDPERLAFLVRLA
                     QRARLVRPDRRRLDPAPALAWLGAPALDQLRGLFIAWSGDDDWNDLEHIPALKIEAGA
                     AWRDDPLAARRAILAHLRAALPEAWHTLDSLAAHIKRHQPDFARTEFDTGYIRDALSG
                     DYLRGFEAWDRVEGELIRHVVTRPLFWMGIVDLGDGLFKITPVGATVLGLVEAPPPSL
                     AEPASHYVVRADATVSVSASRRLDRFQLARVADFVSAGDVYTYRLTPASLARARARKI
                     DTSRIVESLQRASHAEVPPSVIRAVLRWSSKGKEATIERAIVLQVKSPAILKTIQASA
                     KTRGLVGESLGPTSVKVPEKNWQKLRNVLAELGLLVDIEDESG"
     gene            5773..7002
                     /gene="galK"
                     /locus_tag="AC162G_18865"
     CDS             5773..7002
                     /gene="galK"
                     /locus_tag="AC162G_18865"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIR5077924.1"
                     /translation="MNQVDLHALRSAFRESYHSDPDIVVRAPGRVNLLGEHTDYNDGF
                     VLPIAIDRAVTLAASRCSSHLASIVSIEYDESAAFRITEVKPGSEDGWIASPTPRRAG
                     RGSPNATPGWAAYPAGVAWSLREAGCALCGIDAVMTSDVPIGAGLSSSAAVEVAFATA
                     WEHLSGFTLRKPLLARLCQRAENEFVGVMCGIMDQTICAMGRTGHALLLDCRDLSIQH
                     VPLPPGYAVIVADTRVERSLAASAYNERRAQCEEALRLLRRRDPSIAALRDVTPAFLK
                     QHAQDMPDEIGKRAQHVVSENARVLESVEALKRGDVEQFGRAMIASHHSLRDDYEVSC
                     TQLDVMVEAALRIEGVAGARMTGAGFGGCTVSLVREDAVERFLDEVPERYKSATSIDP
                     VVYVCKAAEGASLMTDQ"
     gene            complement(7024..7242)
                     /locus_tag="AC162G_18870"
                     /pseudo
     CDS             complement(7024..7242)
                     /locus_tag="AC162G_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344202.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
     gene            complement(7302..8087)
                     /locus_tag="AC162G_18875"
     CDS             complement(7302..8087)
                     /locus_tag="AC162G_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017251574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR5077925.1"
                     /translation="MAYTTILYEVADGVATIRLNRPDVRNAFNDQMAEELQAALKSAE
                     RDTAVRCVVLTGAGGAFCAGQDLAVMRERGEDVSFREHLGRTFNPIVTKIASLEKPII
                     AAVNGAAAGAGFGIALACDFRYASEKAKFLMAFIGIGLAPDSGTSFFLPRVVGLGRAL
                     EMAYTNEVVDAPTALSLGLVNKVLPADQLMPATMDLAKKLAHGPTKGYGLTKRAMYRA
                     LDIALPEALDYEAHLQEVAGRSADLKEGVSAFLEKRPPQFTGQ"
     gene            complement(8179..8580)
                     /locus_tag="AC162G_18880"
     CDS             complement(8179..8580)
                     /locus_tag="AC162G_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077926.1"
                     /translation="MNLFEPELITIVEGPPPDFQMVGDAWPFSLWEGRTPQAVALTQM
                     RTFDGAAMMDRCLRAWSAARPVMLDFPQMDGLRRKAEVLAARTTSVDEGDVLSLWVVL
                     PVDEVTGGLALDSGDVDPDDLDDRDDPAVDF"
     gene            complement(8645..9736)
                     /locus_tag="AC162G_18885"
     CDS             complement(8645..9736)
                     /locus_tag="AC162G_18885"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR5077927.1"
                     /translation="MRRRIAVLTGGGDAPGLNAVVRAVVKTAIGEYGWEVLGVIDGYD
                     GFIREGGLRTLTTADVRGILPEGGTILGTANRGNPFLRKQAGPDDEPVYVDILDEMVA
                     RIKELDVDGIIVIGGDGTLGASMRLIEHGCLIIGVPKTIDNDLGGTDQTFGFDTALHT
                     ATEAVDKLHTTAASHRRVIVVEVMGRNAGWIAMGAGLAGGADVILIPEIPFRIDDVCK
                     RIRNRTSLGSKFSIVVVAEGALPEGGQQVFRSAGDEMYTARLGGIGAYVSEQIEKIVG
                     QESRVVVLGHLQRGGHPSPYDRLLGTRYGAAAVRLIAEGKIGHMVALRGTEITSVPIA
                     EAVAQPRRVDPKGELVNTAKGLGISMGDD"
     gene            complement(10148..10230)
                     /locus_tag="AC162G_18890"
     tRNA            complement(10148..10230)
                     /locus_tag="AC162G_18890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10195..10197),aa:Leu,seq:taa)
     gene            10649..13405
                     /locus_tag="AC162G_18895"
     CDS             10649..13405
                     /locus_tag="AC162G_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016871929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR5077928.1"
                     /translation="MVSNWYQLSAHDVARKLEADPARGLTDGEARRRLEKFGPNELIE
                     RGGRTSWQILVEQVTNILTLILIGAAVVSIFLGDVLDAVVIMAIVVLNALLGFSQEYR
                     AEQAMAALKRMAVPIVRVRREGTIREISARDLVPGDVVLLETGNVVPADGRVLESVNL
                     RVQESALTGESEAVDKEAGLVFDAEKPLGDRRNMVYSGTIVNYGHGQMIVTETGMQTE
                     LGHIADMIQSVEQEKTPLQRRLDRLGKVLATAALVLVAVIFILGVLRGEPVEEMLLTA
                     VSLAVAAIPEAMTAVVTIALSLGAQRMLKRRALIRKLPAVETLGSVSVICSDKTGTLT
                     QNRMTVTVLDVANHRVDLKQRTDDARFGLTPADGKPISPGATPTIDLLLVSGALCNDA
                     VLVKEENGRRRFHAVGDPTESALVLAAAECGILKDDLDATFPRVAELPFDSIRKRMTT
                     VHRTPQSGEEIPPSLKPVWERRPNEIAPPHYLAFTKGAIDGLLAMTRHVWVDGKLESF
                     DEHWRQRVTAAHDGLAQNGMRVLCVGLRLLDGPPAAQEIEGLENDLILVGLFGMIDPP
                     RPEVMDAVQTCKSAGIRPVMITGDHPLTARHIARQIGITDSGDFVTGQELDDLSETKL
                     EERARDISVFARVSPEHKLKLIRAYQNQGHIVAMTGDGVNDAPALKKADIGVAMGITG
                     TDVSKEAAEMVLLDDNFATIVAAVEEGRIIYDNIRKFVKYLLSCNTSEIAVMLFGPFL
                     GVPLPLLPLQILWMNLVTDGLPALALGVEPAEENVMKRPPYSSEESIFGRGAAQFIVV
                     MGIVMSIVALGVGVWAYQSGDPAWRSLLFTTLIFCQLALALGIRGEETLLFKQGLFSN
                     RSMVWAVLSSIGLQLAVVYVPFLQRVFSTAALSGRDLLIASVAGLVVLLVVEAWKWVR
                     RRRT"
     gene            complement(13640..13798)
                     /locus_tag="AC162G_18900"
     CDS             complement(13640..13798)
                     /locus_tag="AC162G_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077929.1"
                     /translation="MSYIGRFKDTRFRRKAHAVEVANLAFGPLAKGHVRFCDGGAAGN
                     HDLPALAD"
     gene            13925..14326
                     /locus_tag="AC162G_18905"
     CDS             13925..14326
                     /locus_tag="AC162G_18905"
                     /inference="COORDINATES: protein motif:HMM:NF044913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR5077930.1"
                     /translation="MKEAPFIAHDQLGQVPATGGVYAAWLDDEPQCFYVGRAGNLHRR
                     LESHFSGSRASDRFCLYVYDVYIHDERCRISAQMTTTAVNSMTRDWIRRRVRFQWVEL
                     DEGEASNAERELRLKWLPKLNPLEDRPRKRR"
     gene            14478..14723
                     /locus_tag="AC162G_18910"
     CDS             14478..14723
                     /locus_tag="AC162G_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006100124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HIR5077931.1"
                     /translation="MDQRTAWQDFSKLPPEAQRQVLDFIAFLQARYTSARPRRTGKPT
                     KLANEAFIGMWRNREDLNDSTAWVRNARQREWVRRRA"
     gene            14716..15126
                     /locus_tag="AC162G_18915"
     CDS             14716..15126
                     /locus_tag="AC162G_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016516679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR5077932.1"
                     /translation="MPDPVLVDTDVLIDAGRGVRDAVTCLDQIEQRASLAIGAISQME
                     LLIGCRSRAELRSLERFLLRFQILKLDERVSDVAVDLLRRYRLSHGLLIADALIAATA
                     VALDLPFVTKNQRDYQFITGLRLLAYPRPFAAVL"
     gene            complement(15435..>15809)
                     /locus_tag="AC162G_18920"
     CDS             complement(15435..>15809)
                     /locus_tag="AC162G_18920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5077933.1"
                     /translation="ADRTGRRRIAVLACLPIALFIASLVAGLPRLTLYVVGFAAGGGI
                     TAFGASWYALLTGFAPGARRGRIFGIANALSQLGVVFGAMLASTIWQAADLGAAMLSA
                     TIAVLLAGSALLALPGEEEAGA"
BASE COUNT         2935 a         5091 c         4907 g         2876 t
ORIGIN      
        1 gattccatgt gtttcacctc ctgacaacga ttatacccga aaccatagag ccagatgagc
       61 gcgaggccta tccccacatg ttcttgggcg taagccccag ttactcatgc tcaatgattc
      121 tgcgcgcgtg ctcgagtacc actcgaatgc gctcctcgtc catgtccttc gagcgcaagt
      181 agcgctccag cagctccatc ggcgtcatgc cttcggggct gtggccgcct agccgcgcgc
      241 gctccacgcg ctccacgtct ttctgaatcg ccgcaacgta actggctgcc ttgagcacgg
      301 cgcgcacgtc gcgctctcgg agcgaagcct cttgctcggg tcgcatgcgc acgatcacgc
      361 gcacgatcgc gtttgtcacg tcgtgctgcg ccagctcggc ctgcgccgcc atcgtcacgt
      421 cgtcggcttc gcgcacgtcg gcgacgatcg tcttgaacgg gcgcgccttc acttcgacga
      481 actgccaggt cgtctccccc ttgtcgacct cgacccagca gaaccccttg gcttctttct
      541 cctcgccgaa gtcgatgcgc tccaggctgc cggagtagac gatggcaggg tagtcgccct
      601 tcggctggag gctctggtgc ttgtgaatgt gcccgagcgc cacgtagtcc cagcgcgcat
      661 cggccagcac gctcttcagc acggccacgt cgcggccgag catgacgctg cgctcggagc
      721 cgtacgtcgc cccgctgacg gtgaagtggc cggtgagcac ggccggcagc gccggatcga
      781 gctgctcggc cagcgcgcgg acgttgtccc cgacgatttc ctgcagcgcc cgatctagct
      841 cgtcgaccgg cttgccccgg tgctcgtcga gcgacatcag ccggttgcgc atggggtacg
      901 gcgcggtggc aatctggatc gggccgcgct tcgtctcgac gcgatgcagc gcctcttccc
      961 agccgacgac gacgttcggc acggcgagcg tgcgataaat gtcgatgctg ctggccttct
     1021 tctccatggc cggcagatcg tggttgccga cgagcaggat cataggcacg ccggcgtcca
     1081 cgacacgctt gacgcggcgg gcgaactcgc gctgcagcgt cgggctggga tcacgcgtct
     1141 tgaacgcgtc gcctgcgaaa atgaccacgt cgacgtcgcg cgccaggccg tagtcgatca
     1201 cctcgtcgac gcggcgcagg aagtcgagca cgcggctgtt gacgcccgtg gccgaatcga
     1261 tgtgaccgta attctcaatg ccgacgtgga tgtcggcaaa gtgcagcagg cgaatcggct
     1321 cactcatcgg accgcatcac tgcttgagaa tgaggggcag gtacacgttg ttcggacact
     1381 tggcgagcgc aatccgatcg atcacgacgc catccttatt gatcgcccac aaagaaagga
     1441 tgcagccatc gatttgcacc tgcacggtat gatgtacgct ctcagcgtaa gccgagaacg
     1501 gctgcagcgg tggcaagagc gggtaaatat tcgcgccacc gccgccggac acaatgtaaa
     1561 tcacgccatt gataggccgg ctacgctcgt agtcgtgatc gtgcccatta aatacgatgt
     1621 ccacatcacg ctgcgagaat atcggctcaa gtgtattttg catgtccggc aaagtcggat
     1681 cgagccggtg tgggcccgac gtgtacatgg catgatgaaa gtatacgaac ttccatttgg
     1741 ccgtcgaagc tgcaagatcg gattgaagcc actgcaacat ggcactgtct tcgtagcgcg
     1801 gcggcgtcgt gtctagcgcg acaaagtgcg cgtcgcccca atcgaacgag tagtaacgct
     1861 cggttccgag tggattgttc gccggcaggt agaacacgtc cagatatggc tgggcatcag
     1921 gcgtgccata gtcgtgattg ccgagcgagg ggaagatggg cgcgctgtca agcaagttct
     1981 gataggggtc gaagaagtag ggctgatagc cgctcgcctc ccccttgggg tagatcacat
     2041 cacccaggtg gaggatgaaa tccggatcga gcgcatccat ctgggcagcc acgtcgagca
     2101 gctcttgatt cgggatacca ttcgtgcctg ccccgctgtc gccgaccatc acgaagtcga
     2161 acgcgccgcc gggatcgggc gcggtggcaa gcgtcgatgt gtacaacagc gtgttaccgg
     2221 ttccgatccg gtactgatac gtcgtgccgg cgctcaagcc cgtgagcgag acgacatgcg
     2281 tcgtcgccgg agccgtgctg ctgacgaccc gtgtgtagcc catctcgccg tattccacga
     2341 cgctgtcgcc tgccgtcccc gtttgccaca cgacgatcgt gctgtcgcgc gtcgtcgact
     2401 gcaggtacgg tccacgcgtg agcagcggcc cttgcgcggc aacggctggc gccagcaatg
     2461 cagctatgac gagcaatgcg atccacgatc gcctcgttgc caggctgacg atcttccgaa
     2521 ctccagtcat caccactccg tgccctccaa cctccaatct ccaacctcca gaatactcat
     2581 cccaccgtca acccaccatc cagcatgaga cacgcgccgg tcacccagtt cgacgcatcc
     2641 gacgcgagat acaacgccgc acaggctacg tcgtcgggtg tgccgagccg ccgcaggggg
     2701 tagacggacg caatctcgcg ctctttcgcc tccgggtccg gcgcatggtc gataatgtcg
     2761 cgattgagct cggtcttgac gaacccgggg caaatggcgt tggcgcgcac gccttccggc
     2821 ccatgcttca ccgcgatcga ccgcgtgagc atcaccacgc cacccttcga ggcattgtaa
     2881 gcaggcgtgc tgcccgaccc cgcaaacagg ccgagatacg atgcgatggt cacgatccgc
     2941 ccatcgcgct gcgcgatcat gtgcggcagc acgtgcttgc agcacaggaa cggccctttg
     3001 agattgacgg ccatcatccg atcccagtcc gcttcttcga tttccggaac cggagtgcgt
     3061 gcgatgatgc ccgcgctatt gaccaacacg tcgatgcgcc cgaagcattc gagcgtcatc
     3121 gccaccacgc gccgcacctg cgcctcttcg gtcacgtccg tctgcacggt caacacgcac
     3181 ccactcggcg catccagcga cgaggcgact tccttaagat ggtgctcgcg gcgggcggcc
     3241 agcaccaggc gtgcgccggc ctgggcgaac agccgcccga tcgccgcgcc gaatccgctc
     3301 gacgcgccgg tgacaatcgc gacgcgaccg tccaggcgaa atgcgtcgat cgagggcatg
     3361 ccgctcaatc agacttgggg agctgcggag ggttcggcgg gtttcgatcc gtgcggcgcc
     3421 acttgccgtc gatcacgctg ccttccattt cttcgagaat cgcctcgacg atgccacgcg
     3481 ggcagatcat cacaaatagc tggacgccca gcaagaacaa cccgagatcg tcgagctgcc
     3541 ccacgcctag gatgacgtcg ggcagaaaat caaccggcga caagatgtaa gccaccgtcg
     3601 ccaggggcag cagcttgtta gtaaacggga cgcggcgatc ccaaaacaat cgccacgcca
     3661 ggcgcatctg ccggctcagg gccagccaga acgagggcga gcgtcgatcg ttacgcatca
     3721 gtctttctcc tcgccgttgg gcggcaggcg gcgcgtctcc tgcaaatcgt cgatcgtgat
     3781 gtcggccgcg tcgagccagc cccgatgatc gtcgacgacg tccttcggcg ccagcgccag
     3841 gaagatcgtc aagcagatga tgatcacgac cagatcgtcg atgaggccga agggcagcag
     3901 cgcgatttcc ggcatcagct cgatcggcga cagcaggtac acgatgccca ccaacgggat
     3961 caggcgtatc caaatgggca cccggctgtc gcgcatcagc tcgaaaatca ggcgagcctg
     4021 gcgcttggcc gcaacccaaa tgctcgtgcg cggcgtggat cgcttgctca tcacttcctc
     4081 cggcccgaat tataccccat ccgtctgcgc gcggcatcgt tcaagcccgg atgcacctga
     4141 tcgtatataa tcccggcatg cgctctttgc gccagttcct gatcgatacc gagccggcct
     4201 tgctgcgggt catcgcgtcg cgctgggaca tcaacctccc tgctggaagc aagacgcgcg
     4261 acgttgccgc tgtcgtggcg tcctggctgg gcgatccctc gcataccgtg gccatcgtcg
     4321 agcggctgac cccctccgag cgcgaggggc tgcgtgtgct catcgccggc ggcggctcgc
     4381 tcggcgcgtc ggctttcgcc cagcgcttcg gaatcattcg accggtaggc ccggcgcgcc
     4441 tggagcggga tcagccgtgg cgcgcgccga tcagcccggc ggaggggctt tggtaccttg
     4501 ggctgatctt ccgcgcgttc gagcagcgga gcggggccat gcaggacgtg atggtcgcgc
     4561 cggccgagct tcatgcgctc gcggcactgc ttgccgcccc ggcgctgccg gccgatacat
     4621 tgccgccggt cacagcgccg gcatccgtgt cttcttccgc cgcccagctt gccgacgact
     4681 tgtgcacgct tctggcgcat ctgctcaacc cgcacgaacg gtcgtggacg cgccagttgc
     4741 gcgatcccga tcccgagcgg ctggcgtttc tcgtgcgcct cgcgcagcgc gcgcgcctgg
     4801 tgagacccga tcggcgcagg ctcgatcctg cgccggcgct cgcctggctt ggcgcgccgg
     4861 cgctcgacca actgcgcggc ctgttcatcg cctggtcggg cgacgatgac tggaacgatc
     4921 tggagcatat cccggcgctg aagatcgaag ccggcgccgc ctggagggac gatcctctcg
     4981 ccgcgcgccg tgccatcctg gctcacctgc gtgcagctct gcccgaagcc tggcacacgc
     5041 tcgattcgct cgccgcacac atcaagcggc accagcccga cttcgcccgc accgaattcg
     5101 acaccggata catccgtgat gcgctcagcg gcgactatct gcggggattc gaggcatggg
     5161 atcgagtcga aggcgagctg atccggcacg tcgtgacgcg ccccctattc tggatgggaa
     5221 ttgtcgatct gggagacggc cttttcaaga tcactcctgt cggagcgact gttcttgggc
     5281 tggtcgaggc gccgccgccg agcctcgccg agccggcatc acactacgtc gtgcgcgccg
     5341 acgcgacagt gagtgtatcg gcctcgcggc gcctggatcg cttccagctc gcgcgcgtcg
     5401 cggatttcgt ctcggccggc gatgtgtaca cgtatcgcct gacgcccgcc tcgctggccc
     5461 gcgcccgcgc gcgaaaaatc gacacctcgc ggatcgtcga atcgcttcag cgcgccagcc
     5521 atgccgaagt gcctccctcg gtgattcggg ctgtgcttcg ctggagcagc aaaggaaaag
     5581 aggcgacgat cgagcgagcg atcgtcttgc aggtaaagag cccggcgatt ctgaaaacga
     5641 tacaggcttc cgcaaagaca cgagggcttg tcggcgagtc acttggacct acttccgtga
     5701 aggtcccgga gaagaactgg cagaaactca gaaatgtctt ggccgaattg ggactgctgg
     5761 tagatattga agatgaatca ggttgatctg catgcactgc gatcggcttt tcgggaatcg
     5821 tatcactcag atcccgacat cgtcgtccgt gcgccagggc gcgtcaacct gctcggcgag
     5881 cacaccgact acaacgacgg tttcgtcttg cccatcgcca tcgatcgagc ggttacgctc
     5941 gcggcctcac gatgcagcag ccatctggcg tcgatcgtat ctatcgagta cgatgagtcg
     6001 gcggccttcc ggatcacgga ggtgaaaccc gggagtgagg acggctggat tgcctctccc
     6061 acgccgcgaa gagccgggcg cggatcgcca aatgccaccc cgggctgggc agcctatccc
     6121 gccggcgtag cctggtcgct acgcgaagcc ggatgcgccc tctgtggcat cgacgccgtg
     6181 atgacctctg acgtgccgat cggcgcgggg ctaagctcct cggcagcggt cgaagtcgca
     6241 tttgcgaccg cctgggaaca tctcagcggt ttcacgctgc gcaagccgct tctggcccgc
     6301 ctgtgccagc gcgccgagaa cgagttcgtc ggcgtgatgt gcggcatcat ggatcagacc
     6361 atctgcgcga tgggccgtac cgggcacgcg ctcctgctcg actgccgcga cctttcgatc
     6421 caacacgtcc ccctcccgcc aggatatgcc gtgatcgtgg ccgacacgcg cgtggaacga
     6481 tcgctcgcgg cgtcggccta taacgagcgg cgcgcccaat gcgaggaagc ccttcgcctc
     6541 ttgcggaggc gcgatccatc gatcgccgcc ctccgcgacg taacgccggc gtttctgaag
     6601 caacacgcac aagacatgcc cgacgagatc ggaaagcgcg cgcagcacgt cgtgagtgaa
     6661 aatgcgcgcg tgctggaatc ggttgaggca ttgaagcggg gagacgtgga gcagttcgga
     6721 cgggcgatga tcgcgtcaca tcacagcctg cgcgacgact acgaagtgag ctgcacccag
     6781 cttgacgtca tggtggaagc tgcactgcga atcgaagggg tggccggggc gcggatgacg
     6841 ggagcagggt tcggaggctg cacggtgagc ctggtgcgcg aagatgctgt cgaacgtttc
     6901 ctcgacgagg tgccggagcg ctacaagagc gcaaccagca ttgatccagt ggtgtacgtg
     6961 tgcaaggccg cggagggcgc ctcactgatg actgaccagt gatcagtgat cagtcatcag
     7021 tcatcagtca tcagtgtatg tgtagaatcc cttgcctgat ttcctgccca gccaccctgt
     7081 ccgtacgtac tgcctgagga tcggcgccgg tcgatatcgg tcgtcaccgg tctccttctg
     7141 cagaccctcc agcacggcca gcacgacgtc caggccgatc agatcggccc actccagcgg
     7201 gccgcgcggg tagttcgtgt cgagccgcat cgccgtgtcg atggctgcga gcagtaaaag
     7261 tgagtctccc gcagggtcct cctgcgggag atgatttcgt gctactgtcc ggtgaattgc
     7321 ggcgggcgtt tttcgagaaa tgcgctcacg ccctctttga gatcggcgct gcggccggcg
     7381 acctcttgaa ggtgggcttc gtagtcgagc gcctccggca gcgcgatgtc aagcgcgcga
     7441 tacatggccc gcttggtcaa gccgtagcct ttggtcggcc cgtgcgcaag cttcttcgcc
     7501 agatccatgg tggccggcat gagttggtcg gcaggcaaga ccttgttcac caggcccaac
     7561 gaaagcgccg tgggcgcgtc gaccacctcg ttcgtgtagg ccatctccag cgcgcgcccc
     7621 aggccgacga cgcgaggcag aaagaaagac gtgcccgagt caggcgccag cccgatgccg
     7681 atgaaggcca tcaggaactt ggccttctcc gacgcgtatc ggaagtcaca ggccagcgcg
     7741 atcccgaacc cggcgccggc cgccgcaccg ttcacggcgg caataatcgg cttctccagc
     7801 gaggcgatct tcgtgacgat cggattgaag gtgcgcccca ggtgctcgcg gaacgaaacg
     7861 tcctcgcctc gctcgcgcat gacggccagg tcctggccgg cgcagaaggc gccgcccgcg
     7921 cccgtcagca cgacgcagcg caccgccgtg tcgcgctcgg ccgatttcag cgccgcctgc
     7981 aattcctccg ccatctgatc gttgaaggcg ttgcgcacgt cgggccgatt gaggcggatc
     8041 gtcgccactc catcggccac ttcataaaga attgtagtgt aggccatacg gatctctttc
     8101 taggctcacg ctctgcgtac acacgagaac ttcttacaga ctgttccacg cctctaaaga
     8161 aatctcacgt tccacactct aaaaatcgac tgccgggtcg tccctgtcat ccaaatcgtc
     8221 gggatcgacg tcgccggagt ccagcgccag gccgccggtg acttcgtcga caggcagcac
     8281 aacccacaag ctgagaacat cgccttcatc gacgctcgtc gtgcgcgcgg ccagcacctc
     8341 cgctttgcgg cgtaggccgt ccatctgggg aaagtcgagc atcacgggcc gcgctgcgga
     8401 ccaggcgcgc aaacagcgat ccatcatcgc cgcgccgtcg aacgtgcgca tctgggtgag
     8461 tgcgaccgcc tgcggcgtgc gaccctccca cagggagaac ggccaggcgt cgccgaccat
     8521 ctgaaaatcg ggcggcggcc cctcgacaat cgtgatcagc tcgggttcga aaagattcat
     8581 gatgcaagtc cttcgatggg tttgcccctt agagccaatg aagattatac cactctcgcc
     8641 ggcatcaatc gtcgcccatg gagatgccga gaccctttgc ggtgtttacc agctcgcctt
     8701 tcggatcgac gcgcctgggc tgggccacgg cctcggcgat gggcacggac gtgatttcgg
     8761 ttccgcgcag cgccaccatg tgaccgatct tgccttcggc gatgaggcgc actgcggccg
     8821 cgccgtagcg cgtgcccagc agccgatcgt agggcgacgg atggccgccg cgctgcaggt
     8881 gccccagcac cacgacgcgc gattcctggc cgacgatctt ctcgatctgc tcgctgacgt
     8941 aggcgccgat gccgccgagc cgggccgtgt acatctcgtc gccggcgctg cggaacactt
     9001 gctgcccgcc ctcaggcagc gcgccctcgg ccaccacgac gatgctgaac ttgctgccga
     9061 ggctggtgcg atttctgatc cgcttgcaga cgtcgtcgat gcggaatggg atctccggga
     9121 tgaggatcac gtccgcgccg ccggccagac cggcgcccat cgcgatccag cctgcattgc
     9181 gtcccatcac ttcgacgacg atcacccggc gatgcgaggc cgccgtcgtg tgcagcttat
     9241 ccacagcctc ggtcgcggtg tgcagcgcgg tatcgaagcc gaaggtttga tccgtcccgc
     9301 ccaggtcgtt gtcgatcgtc ttgggcacgc cgatgatcag gcagccgtgc tcgatcaggc
     9361 gcatcgacgc gccgagcgtg ccatcgccgc cgatgacgat gatcccgtcg acgtcaagct
     9421 ccttgatgcg cgcgaccatc tcgtccagaa tgtcgacgta gaccggttcg tcgtctggtc
     9481 cggcctgctt gcgcaagaag ggattgcccc ggttcgccgt gccgaggatc gttccgcctt
     9541 cgggcaggat gccgcgcacg tcggccgttg tcagcgtcct caagccgcct tcgcggatga
     9601 agccgtcgta accgtcgatg acgcccagca cctcccagcc gtactcgccg atcgccgtct
     9661 tgacgacggc gcgcacgacg gcattgaggc cgggcgcatc tccaccgccg gtcagcactg
     9721 caatgcgtct tctcatgttt caagtacctc ttgtcactgc tcaccctccc gccggttcct
     9781 ggcatctcgc taaatcaagc acgacaacta ccgcaagcaa actcagtaga tccgatcttc
     9841 agacttcgga agtctcaagg cgtattctcg caggcgatgg tggccggaac tccgagcaat
     9901 ttcgttggca agatgccact tgaagacttc cgaagtctcg accgtggctc aaagttggat
     9961 ttactgagac agcaagagct ctccaatggt aacctgcggg agggttgaac gcgctctcta
    10021 agagtgatta tacctgcctt cgggatgggg cgcacaatac cgtgccagct tccgctcgca
    10081 tcgagccatg tgtcgtgcca tacaaagaaa tctcccgaag gccctcagcg cttcgggagt
    10141 tcaggtgtag gccggagagg attcgaacct ccatgccctt gcgggcgagg gattttaagt
    10201 cccctgcgtc ttcccttccg ccaccggccc gctgcatttc agtgtaacat gcgcccggcg
    10261 actgcgcaag cctcgcctca ccccccaacc ccctctcccg aagcggaacc cccgaaactg
    10321 cgcgagggca ggcagcacac ttcgggagag ggggagtggc gctctcctct ctcctgacga
    10381 cagcacattt agcggacgct tagcgcagcc gaagcgggag gcgaagggtc atcgtcagga
    10441 aaggcccagt cgatcatgag agtgacacgt agtagcgaat tcagtcgcta tccggcgcaa
    10501 acgagtgaaa tcgttactac aaaggtgtat aataataatc aaatccacag aatcacgacg
    10561 aacagcatta cgtgtgacgc actctcgccc tcgcagcgcg gcggagaggg tgggaagtat
    10621 gttgcacatc ccctgacgaa cgggtggcat ggtcagcaac tggtatcaac tcagcgccca
    10681 tgacgtcgcg cgaaagctcg aggccgaccc ggcccggggt ctcacggacg gtgaggccag
    10741 gcgccgcctg gagaagttcg gcccgaacga gctgatcgag cgcggcgggc gcacgtcgtg
    10801 gcaaatcctg gtcgagcaag tcacgaacat cctgacgctc atcctgattg gcgcagccgt
    10861 cgtctcgatc ttcctgggcg acgtcctcga tgccgtcgtg atcatggcca tcgttgtgct
    10921 caacgccttg ctcggcttca gccaggagta tagggctgag caggcgatgg ccgcattgaa
    10981 gcgcatggcc gtgccgatcg tgcgcgtgcg gagggaaggc acgatacgag agatctcggc
    11041 gcgcgacctg gttcccggcg acgtcgtatt gctggagaca ggcaacgtcg tgccggcgga
    11101 tggccgcgtg ctggagagcg tcaacctgcg cgtgcaggaa tccgcactga cgggcgagtc
    11161 cgaggcggtc gacaaggaag ccggcctcgt cttcgacgcg gagaagccgc tcggcgaccg
    11221 gcgcaacatg gtctattccg gcacgatcgt caactacggc cacgggcaga tgatcgtgac
    11281 cgagacgggc atgcagaccg agctgggtca catcgccgat atgatccaat cggtcgagca
    11341 ggaaaagacg cccctgcagc ggcgtctgga caggctgggc aaggtactgg ccacggcggc
    11401 gctggtgctc gtggccgtca tcttcatcct cggcgtgctc agaggcgagc cggtcgagga
    11461 gatgctgctc accgccgtga gcctggccgt cgccgccatc ccggaagcga tgacggccgt
    11521 ggtgacgatc gcgctctcgc tcggcgcgca gcgcatgctc aagcggcggg cgctgatccg
    11581 caagctgccc gccgtcgaga cgctcggctc ggtgagcgtc atttgctccg acaagaccgg
    11641 cacgctgacc caaaaccgga tgacggtgac cgtgctggac gtcgccaacc atcgcgtcga
    11701 cctcaagcag cgcacggacg acgcacgctt cggcctcacg cccgccgacg ggaaaccgat
    11761 cagcccgggc gcgacgccga cgatcgacct gctattagtc agcggcgcgc tgtgcaacga
    11821 cgccgtcctg gtcaaagaag agaatggccg gaggcgcttt cacgccgtcg gcgatccgac
    11881 ggagagcgca ctggtgctgg cggccgccga gtgcggcatc ttgaaagacg atctggacgc
    11941 cacattcccg cgtgtggccg agctgccgtt cgactccatc cgcaagcgca tgacaaccgt
    12001 tcaccgcacg ccgcagtcag gtgaagagat tccacccagc ctgaagcctg tctgggagag
    12061 acggccgaac gagatcgcgc cgcctcacta cctggccttc accaagggtg cgatcgacgg
    12121 cctgctggcg atgacgcggc acgtatgggt cgacggaaag ctcgagtcgt ttgacgagca
    12181 ctggcgccag cgtgtcacgg ctgcccacga cgggctggcg cagaacggca tgcgcgtcct
    12241 gtgcgtcggc ctgcgtctgc tggacggccc acccgccgcg caggaaatcg agggccttga
    12301 aaatgacctg atactcgtgg gcctgtttgg catgatcgac ccgcctcgcc cggaagtcat
    12361 ggatgcggtc cagacgtgca agagcgcggg catccgcccg gtgatgatca ccggcgatca
    12421 tccgctcacc gcgcgtcaca tcgcccggca gatcggcatc accgacagcg gcgacttcgt
    12481 gacgggtcag gaactggatg acctttcaga gacaaagcta gaagagaggg ccagagacat
    12541 atcggtcttc gcccgcgtgt cacctgagca caagctcaag cttatccggg cataccagaa
    12601 ccagggccac atcgtcgcca tgaccggcga cggcgtcaac gacgcccccg cgctgaagaa
    12661 agccgacatc ggcgtggcga tgggcatcac cggcaccgac gtatcgaagg aagccgccga
    12721 gatggtcttg ctcgacgaca actttgcgac gatcgtcgcg gccgtcgaag aaggccggat
    12781 catctacgat aacatccgca agttcgtcaa gtaccttctg agctgcaaca ccagcgagat
    12841 cgccgtgatg ctgttcggcc cgttcctggg cgtgccgctg cctttgctgc cgctgcaaat
    12901 cctgtggatg aacctggtga ccgacggcct gcctgcactg gcgctgggcg tcgagccggc
    12961 ggaagagaac gtgatgaagc gcccgccgta ttcgtcggag gagagcatct tcgggcgcgg
    13021 cgcggcgcag ttcatcgtcg tcatgggcat cgtcatgagc atcgtcgcgc tgggcgtcgg
    13081 cgtgtgggcg tatcagagcg gcgatccggc gtggcgctcg ctgctcttca cgacgctgat
    13141 cttctgccag ttagcactgg cgctcggaat tcgcggcgaa gagacgttgc tgttcaagca
    13201 gggtctcttc tcgaaccgat cgatggtgtg ggccgtgtta tcgtccatcg gtttgcaatt
    13261 ggccgtcgtc tacgtgccat tcctgcagcg cgtgttctcg acggcggcgc tctcgggtcg
    13321 ggacctactc atcgcctctg tcgccggcct ggtcgtgctc ctcgtcgtcg aagcgtggaa
    13381 gtgggtgcgg cggcggcgca cgtgatcaga gacctcacac acggatttcc cacggattgc
    13441 acggattggc aggttcccgg cacagtcatc gtgcaagatg aactcgcgat cggtgtgagt
    13501 cgcaggttcg ctccgcgtcg cgaagaccgg ccaggtctac cacgaccggc ggctaaacga
    13561 ctgacgcgtg cttcacacgg cgcttcacta gatgcttgac gccacggttg atacggaatt
    13621 ccgagagtat ggctaaaatt tagtcggcga gcgcaggtaa gtcgtggttg cccgccgcac
    13681 cgccgtcaca aaaccgaacg tgtcccttcg ccaagggacc gaaggcgagg ttggcaactt
    13741 cgacggcgtg agccttcctg cggaagcgag tatccttgaa gcgcccgatg taggacatgc
    13801 ggctgcacca tgggatcggt acggggcccg ttaggcaact gactgttcta tcgcgatacc
    13861 tgcctgccgc acaccacaga gagtcaggag atccgcattg gactttgaag aactggaacg
    13921 cagggtgaaa gaggcaccct ttatcgcgca cgatcaactc ggccaagttc ctgcaaccgg
    13981 tggtgtttac gctgcttggc ttgacgatga acctcagtgc ttctatgttg gtagagctgg
    14041 caacctacat cggcgtctcg agtcgcattt ctcaggcagt cgcgccagtg accgattctg
    14101 cttgtacgtc tatgacgttt acatacatga cgaacgctgc cggattagcg cacagatgac
    14161 aaccacagca gtcaacagta tgacacgcga ttggattcgc aggcgagtca ggttccaatg
    14221 ggttgaactc gacgagggag aagccagtaa cgccgaacga gagttgcgac tcaagtggct
    14281 gccgaaactg aatcccttgg aagacagacc taggaaacgc cgttgagagg tggctgtagt
    14341 gcgacattat tccacgtgtc attccaacag gcaggctaag ctgcggtcgg ccgggcgcgt
    14401 gaactgaacc gctcgcgccc cgcgccatcg agttgacaat ctagccatta tcgtcgataa
    14461 atagggcgag gtgactcatg gatcagagaa ccgcttggca ggacttctcc aaactacccc
    14521 cagaagcaca gcgtcaggtg ctggatttca tcgcgttctt gcaggcacgc tacacttctg
    14581 cacgcccacg cagaacgggc aaacccacca aactggcgaa cgaagccttc atcggcatgt
    14641 ggcgcaaccg cgaggacttg aatgatagta cagcctgggt gcgtaatgct cggcagcgcg
    14701 aatgggtaag acgccgtgcc tgatccggtc ctggtcgaca ctgacgtgct gatagacgct
    14761 ggccgtggtg tccgtgatgc tgtaacgtgt cttgaccaga tcgaacaacg ggcctctttg
    14821 gcgatcggcg cgatctcgca gatggaactc cttattggtt gtcgcagtcg ggccgaactt
    14881 cgctcactgg aacgcttctt gcttcgcttt cagatcctca agttggatga acgggtctcg
    14941 gatgttgcgg ttgacctctt gcgccggtat cgcctcagtc atggcctgct gattgctgac
    15001 gcgttgattg ctgccactgc tgtggcgctc gacctgccct ttgtgaccaa gaatcagcgc
    15061 gactatcagt tcatcacagg attgcggctg ctggcctatc caaggccatt cgctgccgtg
    15121 ctatgatcat aggactcctc agccaccgtg cgcgcctggc tctggtcagg atggcaggcg
    15181 cctgcctctc gccaccacag aaccaaacgt gcctcttgct aggggactga ggacgaggtt
    15241 ggcaacttcg acgacgtgag ccttcttgcg gaagcgagta tccttgaaga acctggtgcg
    15301 ggaccggcgg cagttgggca actggctgtt ctaccgcgat gcaggtggta cgccgggcga
    15361 acacggcgcc gccctccggt cacggcggag tgagggcggg cttcaagagt ggacgaggag
    15421 ttccatctgc ccgttcatgc cccggcttct tcctcaccgg gcagagccag gagcgcgctc
    15481 ccggcaagca aaacggcaat cgtcgccgac agcatcgccg cgcccaagtc cgcggcctgc
    15541 cagatggtcg aggcgagcat cgctccgaac accaccccca actgcgacag cgcgttggcg
    15601 attccgaaga ttcggccgcg ccgcgcgccc ggcgcgaagc cggtcagcag cgcgtaccag
    15661 gacgcgccga acgccgtgat gccgcctccg gcggcaaatc ccacgacgta cagcgtgagg
    15721 cggggcagac cggcgacgag gctggcaatg aacagcgcaa tcggcagaca cgccagcacc
    15781 gcgatacggc gccgccccgt gcgatcggc
//
LOCUS       DBNHXB010000489         4063 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4189010, whole genome shotgun sequence.
ACCESSION   DBNHXB010000489 DBNHXB010000000
VERSION     DBNHXB010000489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4189010"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="AC162G_18925"
     CDS             complement(<1..491)
                     /locus_tag="AC162G_18925"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR5077934.1"
                     /translation="MTKHTSQSVLVIGSAGLDIKGLASGPLLPGTSNAGRVRRSLGGV
                     ARNVAENLARLDVNVTLLTAVGDDNAGRQILQQASECGIDTSPSIVVPGGRTGSYLAI
                     VDENGSLAVAIGDMGIVESITPRYLNDHRRLFAEASMLAVDANLTPDALSTIFKLAAK
                     HDLP"
     gene            complement(484..1521)
                     /locus_tag="AC162G_18930"
     CDS             complement(484..1521)
                     /locus_tag="AC162G_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077935.1"
                     /translation="MPLKPLEPIDLLSRLEFKKPSQGLDSVRARSIELLKQGKLLEAA
                     DHAREALRDGTERHDRNMQVYAFAYLAAVYAIRKDFREATAHADDCYGLSKQMRNQHN
                     TAIAKALLAMVYQKHLEELSGEFIEPIREVQAEFDSLTSQALAHGQVAEAQKNQASLI
                     EFGEQARRVRWIPALPYTLPLVWLRALDPIKTDPPHQGYLEPVMFVLRMQQEVEREES
                     GNVEPGEIVDALYTARPVPNPNSPDAHPLRPPRLDPHTVYIAVKVDPDMAREPGYQVD
                     DYLLLRGDDPNQLIRQVEEAKGDLTGMEFRRTDDGRFQFRFAPPKDTGGREVSMLMLT
                     VDAILRRIPHD"
     gene            complement(1536..3428)
                     /locus_tag="AC162G_18935"
     CDS             complement(1536..3428)
                     /locus_tag="AC162G_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR5077936.1"
                     /translation="MKAQQRPSLGQVLQAFAADMREWWTRETTHALRRRLYVLVMFVA
                     LGWLGPNFIALSIFPDPPTGISGESDGILCAYDAAPANPPDRLRGPAPIAVVSGITTT
                     LPFDIRNRGSCPWDGATTLRREDSSLIVASQTITPAGTVAPNGVFRADITFAVPPVND
                     ALLNLQYRMYAPDGRSFGAPIKISLLTYPLGGEPKFLQQRNVGNDIVNLIWMALPGVL
                     GFGLAMHRAGRFTWDFYSLKSVQYGVEFVLRRLFNAGPNVTATVKDGQIDWDSRGGAN
                     KERENDVLEKIGGPGTLSVHGGMEVLLERGARYSRIVGPGVTPLGAFERVRALVDARP
                     LNRSKDESAYTKDGIEVKCKTTITFRLMKQKDGEHIPQPQPRISSRQQLSLWLGGGLQ
                     LRLLESWWRWLKSLWHWPMSWWREITLWLGFRPVTTRPPAPPEGLPASPQAVRAIAYE
                     LPAGVGWEGTVSTGIADEIPRRMFDELWAPDSDRKPRHEMIEKLFKENRESLRKRGVD
                     LLDMTIGALEATDKAVDEQRRKYWEAFWASRSHITEAEGEAEALRYMQTVRAEAQADM
                     IQAIAQSFRMLALSGARLPSREVALKMLEVVGRTMKAALEESKGGEPADKAARLLERL
                     QRTVNP"
     gene            complement(3406..>4063)
                     /locus_tag="AC162G_18940"
     CDS             complement(3406..>4063)
                     /locus_tag="AC162G_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR5077937.1"
                     /translation="QVRWPAPYEWSPRQVVLALGQSRVGVEQGESLRWDDIVINEAVK
                     RVHTLIAEYTFDRLIEPHDPRVNPREDIRKRLEDDVKKALAGRGVAVLGIGLTQFVPR
                     DKRVDVQRVEAWKAEWLSRLHVVEAEGLAERYRLIELARAQGQMELITRIAQALEASQ
                     KVGVDDAEEVALSLLEVVERLAAEPGIDERLGSESRAALSGAYKMLGKGLGSESAAAP
                     "
BASE COUNT          710 a         1314 c         1233 g          806 t
ORIGIN      
        1 ggcaggtcgt gcttcgccgc cagcttgaag atcgtgctga gcgcgtcggg ggtgaggttg
       61 gcgtcgacgg ccagcatcga cgcttcggcg aacaggcggc ggtggtcgtt gagatagcgc
      121 ggcgtgatcg attcgacgat ccccatatcg ccgatggcga cggccaggct gccgttctcg
      181 tcgacgatcg ccaggtacga gccggttcgc ccgccgggca cgacgatcga cggcgaagta
      241 tcgatgccgc actcgctcgc ctgttggagg atctgccgtc cggcgttgtc gtcgccgacg
      301 gcggtgagca aggtcacgtt cacgtcgagg cgggccagat tttcggcgac gttgcgcgcc
      361 acgccgccca ggctgcgccg cacacgcccg gcgttagacg tgccgggcag caaaggcccc
      421 gacgccaggc ccttgatgtc gaggccggcc gagccgatga caaggacgga ttgggaggtg
      481 tgtttagtca tgggggatgc gcctcaagat ggcatccacc gtgagcatga gcatcgaaac
      541 ctctctgccg ccggtgtctt ttgggggtgc gaaccggaac tggaaacggc cgtcgtcagt
      601 acgtctgaat tccatgccag taagatcacc tttagcctct tcaacctgtc ttatcaattg
      661 attcggatcg tcaccgcgca gcagcaaata atcatctact tgatagccgg gttctcgtgc
      721 catatcaggg tcaaccttga ccgcgatgta gactgtgtgc gggtccagac gtggtggacg
      781 taaggggtgt gcgtcaggac tattgggatt ggggactggg cgagccgtgt acaaagcgtc
      841 gacgatttca cccggttcga cattgccaga ctcttcgcgc tcaacttcct gctgcattcg
      901 cagcacaaac atcaccggct ccaggtagcc ctgatgaggg ggatcggtct tgatcggatc
      961 cagggcgcgc agccatacaa gcggcagtgt atatggaagg gccgggatcc agcggacgcg
     1021 ccgcgcctgc tcgccgaact cgatcagaga ggcctggttc ttctgtgctt ctgccacctg
     1081 cccgtgggcc agggcctggc tggtcaagct gtcaaactca gcctgcacct ctcggattgg
     1141 ctcgatgaac tcgccgctaa gttcctccaa gtgcttctgg tagaccatcg ccagcagtgc
     1201 cttagcaatg gcggtgttgt gctggttccg catctgctta gataagccat aacagtcatc
     1261 tgcgtgcgca gtggcttccc gaaaatcctt ccgtatcgca tatacagcgg ccagataagc
     1321 gaaagcgtac acttgcatat tgcgatcgtg ccgctcggtg ccgtcgcgca gcgcttcgcg
     1381 ggcgtggtcg gctgcttcga gaagcttccc ttgcttgagc aactcgatcg atcgggcgcg
     1441 caccgaatcc agaccttggc tcggcttctt gaactccaaa cgggagagta gatcgattgg
     1501 ctcaagcggt ttcagtggca tacggcccaa ccctgtcacg ggttgacagt gcgctgcagg
     1561 cgttcgagaa ggcgtgccgc cttgtcggcc ggttccccgc ctttcgattc ttcaagcgct
     1621 gccttcatcg tgcggccaac cacctcgagc atcttcaggg ccacctcgcg cgacggcagg
     1681 cgcgcaccgc tgagcgccag catgcggaaa ctctgcgcta tggcctggat catatcggcc
     1741 tgcgcctcgg cgcgcacagt ctgcatgtag cgcagtgcct ccgcttcgcc ttcggcctcg
     1801 gtgatgtggg atctcgacgc ccagaatgcc tcccaatatt tacggcgctg ctcgtcgacg
     1861 gctttatcgg tcgcttccag cgcgccgatc gtcatgtcga ggaggtcgac gccacgcttg
     1921 cgcagcgatt ctcggttctc tttgaaaagc ttctctatca tctcgtgacg cggcttacgg
     1981 tcagaatcag gcgcccacaa ctcgtcaaac atccgcctgg gtatctcgtc ggcgattccg
     2041 gtggatacgg tgccttccca gcccaccccc gccggcaatt cgtatgcgat ggcgcgcaca
     2101 gcctgcggcg aggcgggcag tccctcaggc ggcgcgggcg gtctcgtcgt gaccggcctg
     2161 aatccaagcc acaatgtgat ttcgcgccac cacgacatag gccaatgcca cagcgacttg
     2221 agccaccgcc accacgactc aagcaagcgc aactgcaggc cccctccgag ccacaaagag
     2281 agttgctgcc gtgacgatat gcgcggctga ggctgcggga tgtgctcgcc gtctttctgc
     2341 ttcatcagcc tgaacgtgat cgtggtcttg catttgacct cgatgccatc tttggtgtag
     2401 gcggactcat ctttcgagcg gttgagcggt cgggcgtcga cgagggcgcg cacgcgctcg
     2461 aacgcgccga gcggcgtcac gcccggcccc acgatgcgcg agtagcgcgc gccgcgctcg
     2521 agcagaactt ccatgcctcc gtgaacggac agcgttcccg gcccgccgat cttctcgagc
     2581 acgtcgttct cgcgctcctt attcgcaccc cctcttgagt cccaatcgat ctgcccgtct
     2641 ttcaccgtgg ccgtcacgtt cgggccggcg ttgaacaaac gcctgagaac gaactcgaca
     2701 ccgtattgga cgctcttgag gctgtagaaa tcccacgtga atcggccggc ccggtgcatg
     2761 gccagcccga aacccaggac gccgggtaat gccatccaga tcagattgac gatgtcattg
     2821 cctacattcc gctgctgaag aaacttcggc tcgccaccca gcggataggt gagcagcgaa
     2881 atcttgatgg gcgcgccgaa gctccggcca tccggcgcat acatgcgata ttggagattc
     2941 aagagggcgt cgttgaccgg cggtacggca aatgtgatgt ctgccctaaa gaccccgttg
     3001 ggcgcgacag tcccggccgg agtgatcgtt tgtgacgcaa cgatcaggct gctgtcctcg
     3061 cggcgcagcg tcgtcgcacc gtcccacgga cacgagccgc ggttgcggat gtcgaacggc
     3121 agcgtcgtgg tgatgcccga aacgactgcg atcggcgccg gtcctcgcag cctgtccggc
     3181 gggtttgccg gagccgcatc gtaggcacag agtatcccat cgctttcgcc ggaaatgccg
     3241 gtcggagggt cggggaagat cgagagcgca atgaagttcg gccccagcca acccagcgcg
     3301 acgaacatga cgaggacgta gagacggcgg cgcagagcat gcgtcgtctc gcgcgtccac
     3361 cactcgcgca tatcggccgc gaatgcctgt agtacctgcc ccaggctagg gcgctgctgc
     3421 gctttcactc cctaaacctt tccccagcat cttatacgca ccgctcagcg ccgcgcgaga
     3481 ttcgctgccc agccgctcgt cgatccctgg ctcggcggcc aggcgctcaa ccacttccag
     3541 caggctcagc gcgacctctt cggcgtcgtc tacgccgacc ttctggctgg cctcgagcgc
     3601 ctgggcgatg cgcgtgatca gctccatttg cccctgcgcg cgagccagct cgatcaaccg
     3661 gtaacgctct gccagccctt cagcctcgac gacgtgtaag cggctgagcc attcggcttt
     3721 ccacgcctcg accctctgca cgtctacccg cttgtcgcgc gggacgaatt gggtgagccc
     3781 gatgcccaac accgcgacgc ccctgcctgc cagcgccttc ttgacgtcgt cttcaagcct
     3841 tttgcgaata tcttccctcg gattgacgcg cgggtcgtgc ggctcgatca gcctgtcaaa
     3901 ggtgtactcg gcgatgaggg tatgcacgcg tttgaccgcc tcgtttatga cgatgtcgtc
     3961 ccagcgcagc gactctccct gctcgactcc cacgcgcgac tgtcctagcg cgaggacgac
     4021 ctggcgcggc gaccattcgt acggcgcagg ccagcgcact tgg
//
LOCUS       DBNHXB010000490        10772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_4189392, whole genome shotgun sequence.
ACCESSION   DBNHXB010000490 DBNHXB010000000
VERSION     DBNHXB010000490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10772
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_4189392"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..2827)
                     /locus_tag="AC162G_18945"
     CDS             complement(281..2827)
                     /locus_tag="AC162G_18945"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014041909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 94 protein"
                     /protein_id="HIR5077938.1"
                     /translation="MRYGYFDDVRHEYVITRPDTPLPWINYLGCEDYFGIISNTAGGY
                     SFYRDARLRRLTRYRYNNAPLDVGGRYLYLRDDAGRDDSASRPYWSPTWQPTRHDLLD
                     YACRHGLGYTMIGSTCQGIEARITYFVPLGETLEVWNLELTNHRDAPASLSIFSAIEF
                     SLWDAHDDATNFQRNFSVGEVETPPLNPPHGNGGDVGGKPRRDGGDTGGVIYHKTEYR
                     ERRNHFAYFACSEPLAGFDTQREAFLGPYRGWDNPLAVERGASAKSIAHGWQTIGSHH
                     VKVNLRPGETRRVIFVLGYHENPPDQKFDPPGSQTINKRTILPTIRKYLDAPAVDAAF
                     DALRVYWNDVLGVLHVDTPDVHTNRMVNIWNAYQCMVTFNISRSASLFESGIGRGMGF
                     RDSNQDLLGFVHMIPSRARQRILDLAATQMENGGAYHQYQPLTKRGNHDIGGGFNDDP
                     LWLILSVSAYLKETGDRSILDECMPFDNLPGTEVTLYDHLRRSIGYTMDRLGPHGLPL
                     IGRADWNDCLNLNTFSEEPGESFQTAAHREGQVAESVFIAGLFVLAAKEMARLSETAN
                     QCEGGCADSPEDYRRLAEQMTAAIREHGWDGAWFLRAYDAFGDKVGSRLCEEGKIFIE
                     PQGMCVMAGIGLDNGRAESALSAVSEHLATPHGIVLQQPAYSRYHVHLGEISSYPPGY
                     KENAGVFCHTNPWIIIAEAIVGHGGRAHDYYTRINPSAREALSELHRCEPYAYAQMIA
                     GRDAPTHGEAKNSWLTGTAAWNYVAITQWILGIRPTLDGLQIAPVIPAAWPGFTAMRV
                     FRGVTYRIEVERVGPGNAVSLTVDGRPIEGNVVPLPSNSEKEVTVKVALR"
     gene            complement(2866..3813)
                     /locus_tag="AC162G_18950"
     CDS             complement(2866..3813)
                     /locus_tag="AC162G_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077939.1"
                     /translation="MLKLDGISKVYRVGTFGGRKLHAVSDVSFDVKDGEVVSLIGESG
                     SGKSTIGKMILRLISTNSGTISFNGVDISTLKGDALKEYYRKVQGVFQDPYSSYNPIF
                     KADRAFRIIKDEFFPEIEDPEWNDRVNRSLEAVGLDPDQVLGKFPHQLSGGQVQRLLI
                     ARALLLDIQFLVADEIISMLDASTRIDVLNLLADLKSRGLSILFITHDLSLAHYISER
                     AVILYRGCIAEMGATEKIFDHPRHPYTKMLMTSVPRLDKKWEEVEVELRANHSELTSG
                     CVYYGRCPVRFDKCLEKPPPIEIEKNHFAACWKEFERNA"
     gene            complement(3818..4768)
                     /locus_tag="AC162G_18955"
     CDS             complement(3818..4768)
                     /locus_tag="AC162G_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5077940.1"
                     /translation="MGLQVENLRVYYQTLKGDVKALDGVTFTVAGGEIMGLAGESGCG
                     KTTLGNSLIHLNSRMRYVEGRVELDGIELPIWDTERMNAFRFKKVSIIPQYAMSALNP
                     TRKIGKITSELLESRGVSFKTTLPELKRRLDLVKLPHDVLSMYPIELSGGMKQRMVMV
                     LSTLLNPSLLVADEITSALDVASQKAVAEMLVEFRNQECCHSAIVITHDISILYQIAD
                     SILIMYAGKLAEKASTDVIINSPRHPYTKLLISSLPEIGVRYSEKKLTGIPGTPPLLL
                     NPPTGCRFRERCPVAFEKCHEEPPFIEVEKGHHVACWKEL"
     gene            complement(4773..5630)
                     /locus_tag="AC162G_18960"
     CDS             complement(4773..5630)
                     /locus_tag="AC162G_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077941.1"
                     /translation="MTTHKKKNEILYFALRNNKLKVGLAVLLFFVVLTFIGPQLTHNK
                     PYDYVGPAAQPPSAEYWFGTTTFGQDVFSQFVFGLQATFLVGIVGGGLATFIGILIGF
                     TAGYRGGIVDEILNMLTNIVLVIPTLAVLLIIVAYLEVRGVFIESVFIGCTAWPWTAR
                     AIRAQTFSLRTRDFVDLARLSGMKSLKIIFTEIAPNMMSYLLLVFILQFGGAVLIAAT
                     LDFIGLGPTNAISLGLMMNSAVLWGALILGIWWWFIPPGIAITAIVGALYVTNVGLDE
                     VFNPKLREM"
     gene            complement(5627..6619)
                     /locus_tag="AC162G_18965"
     CDS             complement(5627..6619)
                     /locus_tag="AC162G_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5077942.1"
                     /translation="MRRYFGRKLLIYLLTFFVAVTIDWLIPRLMPGNPIDVLMARAGS
                     FAEATELMHDYYTKVFGLDVPLGQQYLNFWNALLHGDLGISVYVFPQPVAKVIMNAVP
                     FDLLLLLPAILLSWLAGNWFGAFAARSKRLDNTVLPLGYILTATPYMWLAMLLAWALG
                     IVLKIFPISGGYSYAMSPSFSWAFIWSFLQHWFLPFASLFLVGFGGWAIGMRNMIIYE
                     LEADYSHYLEALGAPKRLVRKYAFNNAVLPQLTGLALQLGVIVAGALVTEVVFSYPGI
                     GYLILQAINTRDYFLLQGCFLFIIIGVLLANFVIDIVYVLVDPRTRTGMQGEVA"
     gene            complement(6691..8484)
                     /locus_tag="AC162G_18970"
     CDS             complement(6691..8484)
                     /locus_tag="AC162G_18970"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5077943.1"
                     /translation="MRKTALSTVSALVIASVLLAACVTPTPATVIQTVEVPKEVVVTQ
                     EVPVEVTPVPAVEAFPRNETLYTGGTQWGPPTGFNPWNLGNYAMGTFGLCYEPLFIYD
                     PLADEFTPWLAESGEWTSDNVYQVKVREGVTWSDGQPFTAADVKYTFDAAQDAPVSIS
                     PVWKFLGSVDQVDDTTVNFTFDQALYQGWSYYLYSTPIVAKHVWETKSVEEITSGLNP
                     EPECTGPYVAESLDQQKVVWAKRDGWWATEALGLDPKPKRIVDIVNSGNNNVALGMVL
                     QGQFDLNNNFLPGISRLVEGGYGVQTYYTGAPYHLSANTAWLLLNLTKKPMDDPAFRK
                     AVAYAIDVSQIVNVVYAGMVLPANPTGLLPNWDKFIDQAVVDELGFSYDPAQAQQILA
                     DAGYTDVDGDGFVEAPDGSKIELKINNPAGWTDWMESNRVIVNGLQAVGINAESNFPD
                     FPEYVSQRNEGTFDMMIANDAQIANTPWRYYLWMARNPIEDIATMQDGNYGRYDNQEV
                     FDLVDQLDQVPTDDTEGMKAIISELQRIFLTDMPMIPLWYNGMWSQVSNAYWTNWPSS
                     TEGAPHWTPVSWRGYWNMTAIRMLTDLEPVQ"
     gene            complement(8762..9775)
                     /locus_tag="AC162G_18975"
     CDS             complement(8762..9775)
                     /locus_tag="AC162G_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HIR5077944.1"
                     /translation="MGTSALRAETKSNLVSGRALPNLPWEDRPDGSREVVWRYSRNPI
                     IPRDAIPTSNSIFNSAVVPFNDGFAGVFRCDDKRRVMNIHRGSSRDAIHWEIEPEPMA
                     FVGGDPEVARFEYRYDPRVTWIEDRYYVSWCNGYHGPTIGLGYTYDFETFHQLENAFL
                     PYNRNGVLFPRRINGKYAMFSRPSDTGHTPFGDIFYSESPDLIHWGKHRFAMGPAGGW
                     QATKIGAGPTPIETTEGWLLFYHGVLTSCNGFVYSFGAALLDLDQPWKVIYRTAPYLM
                     SPQTLYECVGDVPNVVFPCAALVDAPTGRVAIYYGGADTVTGLAFAYVDEIVEFMKHN
                     NLG"
     gene            complement(9792..>10772)
                     /locus_tag="AC162G_18980"
     CDS             complement(9792..>10772)
                     /locus_tag="AC162G_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR5077945.1"
                     /translation="KLSGVSRSTVSRVINSAPNVRDDTRARVWEVVRRLNYHPNAAAR
                     GLAAGRTRVLGLVIPTGVAALFTDPYFPILIQGVTSACNAHDHSVMLWLAEPEYERRA
                     IRQIMHNGLIDGVIVAAMLVDDPLVEALTNSEMPFILVGRHPTNPKVNYIDVDNRTGT
                     VAMVEHLLQAGRRRIATITGPQNVIAGSHRREGYLSALSAHNVKPEDDLIVEGDFSEM
                     GGYSAMRQLLPRHPDAVFAASDIMAVGALRAIREAGLRVPEDIAVTGFDDMPFAARTD
                     PPLTTVRQPIQRTGALAAETLIDLIEHPDSQPRRVVLPTELVVRVSCGAA"
BASE COUNT         2189 a         3154 c         3187 g         2242 t
ORIGIN      
        1 atgcggtaat cgccgtaggg tgtcgcccac ttcaggtcgg gcaactcggc cagcaggttc
       61 tcgtccgagc cgttcacgaa cagccgcacg atgcgcgggc cggcctcggc cagaaactcc
      121 aggcgaatgt gcgcgttgct gatgaaacgc gtcgggagtc cgtgaaagcc agagatcatg
      181 ttcgtggtga ttcgtgaaga ttcgtggatc gattcaagcc catctctgcg atcacgcgat
      241 gcgtccgccc gtcgtcgaag atcagtcccg tgtccgtttc ttatctcagt gctactttga
      301 ctgtgacttc cttctcactg ttcgacggga gcggcacgac gttgccttcg atcgggcgac
      361 cgtcgactgt cagcgagacg gcgttccctg gcccgacccg ctccacctca atccgatacg
      421 tcacgccgcg aaagacgcgc atcgccgtga agccgggcca cgcggcgggg atgaccggcg
      481 cgatctgcag cccatccagc gtgggacgga tgccgagaat ccattgcgtg atcgccacgt
      541 agttccacgc cgcggtgccg gtcagccacg agtttttcgc ctcgccgtgc gtcggcgcgt
      601 cgcgtccggc gatcatctgg gcgtaggcat acggctcgca gcgatgcaac tcgctcagtg
      661 cttcgcgcgc cgaagggttg atgcgcgtgt agtaatcgtg cgcgcggccg ccgtgcccga
      721 cgatcgcttc ggcgatgatg atccatgggt tggtgtggca gaagaccccc gcgttctcct
      781 tgtagccggg cggataggac gagatttcgc cgagatgcac gtggtagcgc gagtaggccg
      841 gttgttgaag gacgatgccg tgcggcgtgg cgaggtgttc gctcacagcc gacagcgccg
      901 attcagcccg gccattgtcc agcccgatcc cggccatcac gcacatcccc tgcggctcga
      961 tgaaaatttt tccttcttcg cagagacgcg atccgacctt gtcgccgaag gcatcgtagg
     1021 cgcgcagaaa ccacgcgccg tcccagccgt gctcccgaat ggccgccgtc atctgctcgg
     1081 ccagtcggcg atagtcctcc ggcgaatcgg cgcatccgcc ttcgcactga ttcgccgtct
     1141 cactgaggcg cgccatctct ttcgccgcca gcacgaacag tccggcgatg aaaaccgact
     1201 cggccacctg accctcgcga tgcgccgccg tctggaacga ttcgcccggc tcttcggaga
     1261 aggtattgag attcaagcag tcattccaat cggcgcgacc gatcagcggc agaccgtgcg
     1321 ggccgagtcg atccatggtg tagccgatgc tgcgccgcaa gtgatcgtac agcgtcactt
     1381 ccgtgccggg caggttgtcg aacggcatgc actcgtcgag aatgctccta tcgcctgtct
     1441 ctttcagata agccgacaca ctcaagatca gccacagcgg atcgtcgttg aagccgccgc
     1501 cgatgtcgtg gttgccccgt ttggtcagcg gctgatattg atgatacgcg ccgccgtttt
     1561 ccatctgcgt tgcggcgagg tcgaggatgc gctgccgcgc ccgcgaaggg atcatatgga
     1621 cgaagccgag caggtcctgg ttcgagtcgc ggaagcccat gccgcgcccg atgcccgact
     1681 cgaacaacga cgccgagcgc gagatgttga acgtcaccat gcactggtaa gcgttccaaa
     1741 tgttgaccat gcgattggtg tgaacgtcgg gcgtgtccac gtgcaacacg ccgagcacat
     1801 cattccagta cacgcgcagc gcatcgaacg cagcatcgac ggcgggcgca tcgaggtact
     1861 ttctgatagt tggcaggatg gttcgcttgt tgatcgtctg cgaaccgggc ggatcgaatt
     1921 tctgatcggg cgggttctcg tgatagccca gcacgaagat cacgcggcgc gtctcgcccg
     1981 gccggaggtt cactttgacg tgatgcgatc cgatcgtctg ccagccgtgc gcgatcgact
     2041 tggccgacgc gccgcgctcg accgcgagcg gattgtccca cccgcgatac gggccgagga
     2101 atgcctcgcg ctgcgtgtcg aatccggcca gcggctcgga gcaggcgaag taggcgaagt
     2161 ggttgcgccg ctcacggtac tcggtcttgt ggtagatcac cccccctgtg tcccccccgt
     2221 cgcgacgggg cttcccccct acgtcccccc cgttaccatg ggggggattg agggggggcg
     2281 tttccacttc gccgacgctg aagttgcgct gaaagttcgt cgcatcgtcg tgcgcgtccc
     2341 atagggagaa ctcgatcgcg gagaagatcg acagcgacgc gggcgcgtcg cggtgattgg
     2401 ttaattcgag attccagact tcgagggttt cgccgagcgg gacgaagtac gtgatgcgcg
     2461 cttcaatgcc ctgacaagtc gatccgatca tcgtgtagcc gaggccgtgc cggcaggcgt
     2521 agtcgagcag atcgtgccgg gtcggctgcc acgtcggcga ccagtagggg cgactcgccg
     2581 agtcgtcgcg accggcgtca tcgcgcagat agaggtatcg cccgccgaca tcgagcggcg
     2641 cattgttgta gcgatagcgc gtcagtcggc gcaggcgcgc gtcgcggtag aatgagtaac
     2701 cgcccgccgt gttgctgata atgccaaagt agtcctcgca gccgaggtag ttgatccagg
     2761 gcaggggagt atcgggccgc gtgatgacgt attcgtggcg cacgtcgtcg aagtatccgt
     2821 agcgcataaa aagtcctgtg atgtcgtttt gatatttcgt cgagctcaag cattcctttc
     2881 gaattccttc caacacgccg cgaagtggtt cttctcgatt tcaatgggcg ggggcttttc
     2941 caggcactta tcaaacctga cgggacatct tccgtaatag acacagccgc tggtgagctc
     3001 cgaatgattt gctctcagct ccacctcgac ctcttcccat ttcttatcca ggcgtggaac
     3061 ggaggtcatg agcatcttgg tgtagggatg ccgtggatgg tcaaatatct tctcggtcgc
     3121 gcccatctcg gcgatacagc cgcgatagag aatgaccgct ctttcactga tgtagtgagc
     3181 cagcgagaga tcatgcgtaa tgaaaaggat agacaggccg cgcgatttga ggtcggccag
     3241 caggttgaga acgtcgatgc gcgtggaggc atcgagcatg ctgatgatct catctgccac
     3301 gagaaactga atgtcgagca acagcgcgcg ggcgatcagc agacgttgaa cctgcccacc
     3361 actgagctgg tggggaaatt tgcccaggac ttgatcgggg tccagcccga cggcctctaa
     3421 cgaacggttc accctgtcgt tccattccgg gtcttctatc tccggaaaaa attcgtcttt
     3481 gatgatcctg aaagcccgat ccgctttgaa gattgggtta taggagctat acgggtcttg
     3541 aaaaacaccc tgtactttcc gatagtactc tttcagtgcg tcgcctttga gggtggagat
     3601 gtccaccccg ttgaatgaaa tcgtccccga gtttgtcgag atgagacgca ggatcatctt
     3661 cccgattgtg cttttcccgc tgccgctctc accgatcaac gagacgacct ctccgtcttt
     3721 gacatcgaag ctgacgtcgc tcaccgcgtg gagcttcctc ccgccgaagg ttccaacccg
     3781 atagaccttc gatattccgt caagcttgag cattctctca cagctccttc caacaggcga
     3841 cgtggtggcc tttttcgact tcgatgaagg gcggctcctc gtggcatttc tcaaaagcga
     3901 cgggacatcg ttctcggaag cgacacccgg ttggaggatt gagcagcagc gggggggttc
     3961 cgggaatgcc cgtcagtttt ttctcggaat acctcactcc gatttctggt aaggatgaaa
     4021 tcaacagttt tgtatacgga tgccgggggg aattgatgat cacatcggtc gaggcttttt
     4081 cagccagttt gcctgcgtac atgatcagaa tgctgtctgc gatctggtac aggatcgaaa
     4141 tgtcatgggt tattacgatc gcgctatgac agcattcctg gttccgaaat tcgaccagca
     4201 tctcggcgac agccttttgg gaggccacgt cgagcgccga agtgatctcg tccgcgacga
     4261 gcagtgaagg attcagcaaa gtagacagaa ccatcaccat tctctgcttc ataccgcctg
     4321 acagctcgat cggatacata ctcagcacat catgcggcag tttaaccaga tcgagtctcc
     4381 gctttaactc cggcagcgtg gtcttgaagc tgacccctct cgattcgaga agttcggaag
     4441 tgatctttcc gatctttcgc gtcggattca gggcgctcat ggcatactgc gggatgatcg
     4501 aaaccttctt gaatctgaag gcattcatcc tttccgtgtc ccagatgggt agctctatgc
     4561 cgtcgagttc cactcggcct tcgacatatc tcatgcggga gttcaagtga atcaggctgt
     4621 tgcccaacgt ggtcttgccg cagcccgatt cgccggccag ccccatgatc tccccacccg
     4681 cgacggtgaa ggttacgcca tccagcgcct tgacgtcgcc ttttagggtt tgatagtaga
     4741 ctctgaggtt ttctacttgt agacccacgc cgtcacatct ccctgagttt ggggttgaag
     4801 acttcgtcca gtcccacgtt cgtgacgtac agcgcgccga cgatagcggt gatcgcaatg
     4861 cccggcggaa tgaaccacca ccatatcccc agaatcagag cgccccacag caccgcgctg
     4921 ttcatcatga gaccgagcga aatcgcattc gtcggaccga gaccgatgaa atccaacgtg
     4981 gccgcaatga gcaccgctcc tccaaactgg aggatgaaga ccagtaatag ataggacatc
     5041 atgttcggcg cgatctcggt gaagatgatc ttgagcgact tcattccact cagccgcgcc
     5101 agatcgacaa aatccctggt gcgcagtgaa aacgtctggg cgcggatggc tctggctgtc
     5161 cagggccagg cggtgcatcc gatgaagaca ctctctatga agacgccccg gacttcaaga
     5221 taggcgacga tgatgagaag gacggccagc gtcggaataa ccaggacgat gttggtgagc
     5281 atgttgagaa tctcgtccac gatgccgccg cgataaccgg ccgtgaagcc aatcagaatt
     5341 ccgatgaagg tggcgagtcc gccgccgacg atgcccacca ggaacgtggc ttgaaggccg
     5401 aatacaaact gtgagaaaac atcctgtccg aaggtcgtgg tcccgaacca gtactcggct
     5461 gagggcggct gcgccgccgg acccacgtaa tcatacggtt tgttatgcgt gagctgtggg
     5521 ccaatgaatg tcaggacaac gaagaagagc aggacggcca ggccaacctt gagtttgtta
     5581 ttcctcagcg caaagtagag gatttcattt ttcttcttgt gtgtcgtcat gcgacttcac
     5641 cctgcatccc ggttcgggtt ctcggatcga caaggacata aacgatatcg atgacgaaat
     5701 tggcgagcaa cacgccgatg atgatgaaca ggaagcatcc ctggagcagg aaataatccc
     5761 tggtattaat agcctgaaga atcaggtacc cgatcccggg gtaggagaac acgacttctg
     5821 ttaccagggc gcccgcaacg atgacaccta actgaagcgc caggccggtg agttggggaa
     5881 ggaccgcgtt attgaaggcg tattttctga ccagcctttt gggtgcccca agcgcttcga
     5941 gatagtgaga ataatccgct tcgagctcat aaatgatcat gttcctcatg ccaatcgccc
     6001 agccaccaaa acccaccagg aaaagagagg cgaaaggtag gaaccagtgc tgcaagaagc
     6061 tccagatgaa agcccatgag aacgagggcg acatggcgta gctgtacccg cccgagatcg
     6121 gaaagatttt caacacgatt ccaagtgccc aggccagcaa catcgccagc cacatgtagg
     6181 gagtcgccgt caggatgtag ccgagcggca atacggtgtt gtccagccgc ttggagcgcg
     6241 ctgcgaaagc gccgaaccag tttcccgcca accagctcaa caggatcgcc ggaagcagaa
     6301 gcaggaggtc aaagggcacg gcgttcatga tgacttttgc taccggctgc ggaaatacat
     6361 acacgcttat gccgaggtcg ccgtggagaa gggcattcca gaaattaagg tactgttgcc
     6421 cgagaggtac gtctaatcca aagactttcg tgtaataatc atgcatcaac tcagtcgcct
     6481 cagcaaacga gccggccctt gccatcaata cgtcaatcgg attgccgggc atgaggcgcg
     6541 ggattaacca atcgatggtc accgcaacga aaaacgtgag gagatatatg aggagtttcc
     6601 ttccgaaata gcgtctcacc ctgcctccta tgcctgtaga agaaccgccc ctcccgcaag
     6661 cgcagaaggg aggggcggtg attcaacaaa ctactgcacc ggctccaggt cggtgagcat
     6721 gcggatggcg gtcatgttcc agtagccgcg ccagctgacc ggcgtccagt gaggggcgcc
     6781 ctctgtggac gacggccagt tggtccagta cgcattgctg acctgggacc acataccgtt
     6841 gtaccacagc gggatcatcg gcatgtcggt gaggaagatc cgctggagct cggatatgat
     6901 cgccttcatg ccttcggtgt catcggtggg cacctggtct agctggtcca ccagatcgaa
     6961 gacttcctgg ttgtcatagc ggccgtagtt gccgtcctgc atcgtcgcaa tatcctcgat
     7021 gggattccgg gccatccaga gatagtaccg ccacggggtg ttggcgatct gcgcgtcgtt
     7081 ggcaatcatc atgtcaaacg taccctcgtt gcgctgagac acatactccg ggaagtccgg
     7141 gaaattggat tcggcgttga tgcctacggc ctgcaggcca ttgacaatga ccctgttcga
     7201 ctccatccag tccgtccagc cagccggatt gttgatcttc aactcgatct tggagccgtc
     7261 cggcgcttcg acgaagccgt cgccatcaac atccgtgtag ccggcatcgg ccaggatttg
     7321 ctgggcttgg gcgggatcgt agctgaagcc cagttcatcc accaccgcct ggtcgatgaa
     7381 cttgtcccag ttcggcagca gaccggtcgg attggccggc aggaccatgc cggcatagac
     7441 cacattgacg atttggctta cgtcaatcgc ataggctacc gccttgcgga aagccgggtc
     7501 gtccatcggc ttcttcgtga gattcagcag cagccaggcc gtgttggcgg agagatggta
     7561 cggggcaccc gtatagtacg tctgaacgcc gtagccgcct tcgacgagtc tggagatgcc
     7621 cggcaggaag ttgttgttca gatcgaactg gccctgcagc accatgccga gcgcgacgtt
     7681 gttgttgccg ctgttgacga tgtcgacgat ccgcttgggt ttgggatcca gaccgagcgc
     7741 ctccgtggcc caccagccgt ctcgcttggc ccagaccact ttctgctgat cgagggactc
     7801 agccacgtaa ggccccgtgc attcaggctc cgggttgagt ccggaggtga tctcctccac
     7861 cgatttggtc tcccagacat gcttggcgac gatcggggtc gagtacaggt agtacgacca
     7921 gccctgatag agagcctggt cgaaggtgaa gttcaccgtg gtgtcatcca cctgatcgac
     7981 actgcccagg aacttccata ccgggctgat gctgaccggc gcgtcttggg ccgcgtcaaa
     8041 ggtgtatttc acgtcggccg ccgtgaacgg ttggccgtca gaccacgtga cgccctcgcg
     8101 caccttcacc tggtagacat tgtcgctcgt ccactcaccg ctttctgcca gccagggtgt
     8161 gaattcatcg gccagcgggt catagatgaa cagcggttca tagcagagcc caaaggttcc
     8221 catggcatag ttcccaagat tccagggatt aaagcctgtg ggtgggcccc actgtgttcc
     8281 gccggtgtag agggtttcgt tccgtgggaa agcttccacg gcaggtaccg gagtgacctc
     8341 cacgggcact tcttgggtca ccacgacttc cttgggaact tccaccgtct ggatgacagt
     8401 ggctggcgtg ggcgtgacgc aggcggcgag caatacgctg gcgattacca gcgcgctcac
     8461 ggtagataga gctgtcttac gcatttcagt gctccttttc tcctcactgg atcatacagg
     8521 tcgagttgtg ttatagtctc tgcccagaga ccccatcggt gtgcgcgcaa gaagggggtt
     8581 ctccggacct ctggcatctc tggtacaggt gccagaggtt tctcgtctaa acggcttctc
     8641 gcatttctca gtccggctcg attcacctcc ttctcgtcag ccacaactta cccaatcggc
     8701 gggtagacga actgcacgcg gcacggctat tcagcgtggc cgtgaagcgc gtctgcccgc
     8761 tttatcccag gttgttgtgc ttcatgaact ccacgatctc atcgacatac gcaaacgcca
     8821 aaccggtgac ggtatccgcg ccgccgtaat aaatcgccac gcgtccggtt ggcgcatcga
     8881 ccagtgccgc gcacgggaac accacattgg gcacatcgcc cacgcattca tacagagtct
     8941 gcggtgacat caaatagggc gcagtgcgat atatcacctt ccagggttga tcgagatcga
     9001 gcagggcagc gccgaagctg taaacgaagc cattgcagga ggtcaacacg ccgtggtaga
     9061 acagcaacca gccttcggtc gtctcgatcg gcgtcggccc cgccccgatc ttggtcgcct
     9121 gccaaccgcc ggccggcccc atggcaaacc gatgtttgcc ccagtgaatc aggtcggggc
     9181 tttcgctgta gaagatgtcg ccaaacggcg tatggcccgt gtcgctgggc cggctgaaca
     9241 tcgcgtactt gccgttgatc cggcggggga acagcacccc gttgcggttg taaggcaaga
     9301 aggcgttttc aagttgatgg aacgtctcga agtcgtaagt gtaccccagg ccaatcgtcg
     9361 gcccgtggta accgttgcac cacgacacat agtagcgatc ctcgatccag gtcacgcgcg
     9421 gatcgtagcg atactcgaag cgcgctactt cgggatcgcc gcccacgaac gccatcggtt
     9481 ccggttcgat ctcccagtga atcgcgtcac ggctcgagcc gcgatggatg ttcatcacgc
     9541 gccgcttgtc atcgcagcgg aacacgccgg cgaaaccgtc gttaaagggc actacggcgc
     9601 tgttgaaaat gctgttggag gtcgggatcg cgtcgcgagg aatgatcggg tttcggctgt
     9661 agcgccagac cacctcacga ctgccatctg gccgatcttc ccaggggagg ttaggcaacg
     9721 ctcgaccact aacgaggttt gacttagttt cggcgcgtaa ggcagaagta cccatgttcg
     9781 atccccggcg gtcaagccgc gccgcacgat acccgcacaa ccaactcagt gggcaaaacc
     9841 acgcggcggg gctgtgaatc gggatgttcg atcagatcga tgagcgtctc agccgccaac
     9901 gcaccggtgc gctggatagg ctggcgcacg gtggtcaggg gcggatcggt gcgtgcggcg
     9961 aaaggcatat cgtcaaagcc ggtcaccgcg atatcttccg gcacgcgcag cccggcttca
    10021 cgaatcgcgc gcagcgcgcc cacagccatg atgtcgctcg ccgcgaatac cgcatcgggg
    10081 tggcgcggca gcaactgccg catggcgcta tagccgccca tctcgctgaa gtcgccttcg
    10141 acgatcaggt catcttcggg cttcacgttg tgcgcactta acgccgacag atagccttca
    10201 cgccgatggg atccggcaat cacgttttgt gggcctgtaa tggtcgcaat ccggcgacgg
    10261 ccagcttgga gtagatgttc caccatcgct accgtaccgg tcctgttgtc gacgtcgatg
    10321 tagttcactt tgggattagt gggatggcgt ccgaccagga tgaacggcat ctcgctgttc
    10381 gtcagcgctt cgaccagtgg atcgtccacg agcattgccg cgacgatcac gccatcgatc
    10441 agaccgttgt gcatgatctg acgaatagcg cgccgttcgt attccggttc agccagccat
    10501 agcatgaccg aatggtcgtg ggcgttgcat gctgaagtca cgccctggat caggatgggg
    10561 aagtaggggt cggtgaataa cgccgccact ccggttggaa tgacgagccc gagcactctg
    10621 gtgcgtccgg cggccagacc tcgagctgcc gcgttggggt gatagttcag cctgcgcaca
    10681 acctcccaga cgcgcgcacg cgtgtcgtcg cgaacattgg gggcgctgtt gatgactcga
    10741 gagacagtcg agcgggacac cccgctgagt tt
//
LOCUS       DBNHXB010000491         5648 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_666573, whole genome shotgun sequence.
ACCESSION   DBNHXB010000491 DBNHXB010000000
VERSION     DBNHXB010000491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_666573"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            470..1555
                     /locus_tag="AC162G_18985"
     CDS             470..1555
                     /locus_tag="AC162G_18985"
                     /inference="COORDINATES: protein motif:HMM:NF028488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5077946.1"
                     /translation="MGFRVISKGAMSRFVASLQTDYRVVGPRPVDGQYIFDDVRSTDE
                     LHLDYTQTVLPPKKYLLPQREELLRFKTDGSQIEPTFDGRQTAVLGVHTCDLHAIQLL
                     DKVFNTGTVDQHYRKRRENTVLISVECLKPCSKHSFCKSMGTLSATDGFDLHLTDLSV
                     PDDAAGTVEYAIDVGSDRGRALLDGHAETRPATEADYQRMNDVLAEKWPRFWYRLDFD
                     VSELPSLLRVSYKSPLWEELGRRCLACGSCTNVCPTCYCFNVQDEVDFALSLGKRTRV
                     WDSCQLDEFATVAGGHNFRPARAARQRHRFLRKGQYQTDAYGLLGCVGCGRCAQACLA
                     KINPVDTFNALYHERVDARHDARNSLT"
     gene            1892..3211
                     /locus_tag="AC162G_18990"
     CDS             1892..3211
                     /locus_tag="AC162G_18990"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HIR5077947.1"
                     /translation="MTGSSRLPGFYNLPLTRRLEVVAQEVGLTPEEAAVMSGMSGLSS
                     DAADHMVENAVGVFGLPLGIATNFLVNGREVLVPMAIEEPSVIAGASFAARLIREGGG
                     FTATSDEPVMIAQVQILDLVNPHAARLALLANKQRLLDFANTTDPVITGLGGGARDIE
                     ARVVEQAPVGPMLVMHLLYDCRDAMGANTVNTAAEALRPMIEEITGGRVGLRILSNLA
                     DRRLARAMAKVPPAALAFDEYDGEVVRDRIVEAWAFAAADPYRAATHNKGIMNGVDAV
                     VIATGNDWRAIEAGAHAYAARSGRYTSLTHWGRDAEGNLVGTLEMPMAVGIVGGATKV
                     HPAARGALKIVGIRTARELAEIIVSVGLAQNLAAIRALATEGIQRGHMTLHARQLASA
                     AGATGDLIERIAAQMVAEKSIRLDRAEALVREIAETVERAKSVISDQ"
     gene            3354..4409
                     /locus_tag="AC162G_18995"
     CDS             3354..4409
                     /locus_tag="AC162G_18995"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HIR5077948.1"
                     /translation="MGLMKPNRPVGIAGYGAYVPRYRLPASEVSRVWTDGTEGTPIAE
                     KAVPGLDEDVATMSIEAARNALARAAIDAEQIGAVWVGSESHPYAVKPTSTIVAEAIG
                     AIPNTQAADWQFACKAGTEALQAAIGFVASGMARYALSIGMDTAQGRPGDALEYTAGA
                     GGAAVIVGPAEEGLAEIEASLSFVTDTPDFWRRPYAHYPEHASRFTGEPAYFKHITSA
                     GEQLMQELATRPEDYTYAVFHQPNVKFPQRAAQMLGFKPEQFEAGLLVNVIGNTYAGS
                     CMIGLTAILDQAKPGDRIFMVSFGSGAGSDAFSITVTDKIAGRQERATATRDYIARRV
                     LIDYATYARYRKKLLMQ"
     gene            4522..>5648
                     /locus_tag="AC162G_19000"
     CDS             4522..>5648
                     /locus_tag="AC162G_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014123186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR5077949.1"
                     /translation="MRDVSIIGIGQTRVGEHWDTSLRHLALEAIKGAALEANVDRVDG
                     LFVGNMLAGELSGQGNLGALIADFVGLRGVEAVTVEAASASGAAAVRQAYLAVAGGLM
                     DFALVVGVEKLSDVVGSPAVTAQATATDSDYEAAHGATPLAIAAMMMRRYMHEYDASL
                     ADFAAFSVNAHLNASTNPYAMFHNKLSVQSFVKAPMVADPINLFDAAAPADGAAALIL
                     VRSEWARGRGNGAARVRIAASAAATDALAIADRSDPLWLAAAEASAKRAYHQAGVTAR
                     DIQFAELHDAATVLAALSLEACGFAGRGGGVNFAKGGGIARGGQTPTGTLGGLKGRGD
                     PGGATGVYQIVEAVQQLRGQAGPNQIADARLGLAQSLGANGA"
BASE COUNT         1063 a         1750 c         1897 g          938 t
ORIGIN      
        1 caacacggcg aagcacctca ggctggcagg cgcacctgca tcgaacagcg ctctgaattc
       61 tggtgttatt tgctgaggac tgatttggat catgtgtgta agggcgccgc aatgacagtg
      121 tacatcggaa tcgaacggag tcaagctaaa caccacggaa ctacttagcc gtcattgcga
      181 ccccgctgag attgcttcgc aaaaacacgc tcgcaatgac gtgcggggca ggtgagcgcc
      241 gtgtgcgacg aagcaatctc agccgccctt agcgaatacg gctgtccgtg ccggagattg
      301 cttcgcaaaa gacgctcgca atgacgccgg cgggatgctg ccagttgacc tatgtccctt
      361 gtgatttatg tcactagtgg taagagccgg tgagtgtatc atatatgatg gagattggtg
      421 tgcggaactg aaacttgggg ttggcgcttc aggcgaatga ggtgagccta tgggtttccg
      481 agtcatctca aaaggggcga tgagccgctt cgtcgcctcg ctgcaaaccg attatcgggt
      541 cgtggggccg agacccgtcg atgggcaata catcttcgac gatgtccggt cgacggacga
      601 gctgcatctg gattacacgc agaccgtgct cccgcccaag aagtaccttt tgccacagcg
      661 cgaggaactg ctgcgtttca agacggacgg cagccagatt gagccgacct tcgacggacg
      721 gcaaacggcg gtgctgggcg tgcacacctg cgatctgcat gccatccagt tgctggacaa
      781 ggtgttcaac accggcaccg tcgaccagca ctaccggaaa cggcgagaga acaccgtgct
      841 catcagcgtc gagtgtctga agccgtgcag caagcactcg ttctgcaaga gcatgggcac
      901 gttgtccgcc acagatggat tcgacctaca cctgaccgat ctgagcgtgc ccgacgatgc
      961 cgccggaaca gtcgagtacg cgattgacgt cggaagcgat cgaggccggg cgctgctgga
     1021 tgggcacgcc gaaacccgcc cggcgaccga agcggactac cagcgcatga acgacgtcct
     1081 ggccgagaag tggccgcgct tctggtaccg cctggacttc gacgtcagcg agctgccgtc
     1141 gctgctgcgc gtgagctaca agagtccgct gtgggaggag ctgggccgtc gctgcctggc
     1201 gtgcggatcg tgcaccaacg tctgcccgac gtgttattgc ttcaacgtgc aggacgaagt
     1261 ggacttcgct ctgtcgctcg gcaagcgcac gcgcgtctgg gattcgtgcc agctcgacga
     1321 gtttgccacg gtcgcgggcg ggcacaattt ccgtccggcg cgcgccgcgc ggcagcggca
     1381 ccgcttcctg cgcaagggcc aatatcagac ggatgcctac ggcttgttgg ggtgtgtggg
     1441 ctgcgggcgc tgtgcgcaag cttgcctggc gaagatcaac ccggtcgaca ccttcaacgc
     1501 gctgtatcac gagcgcgtgg atgcccgcca cgacgcccgc aacagcctga cctgacacac
     1561 ggatttccca cggattacac ggactggcaa gtattccggc acggtcaagg cgcaaaatga
     1621 actcgtaatg gctcaggtcg atagcgggat acttgccaag ttcggagcca aaaaacgtcc
     1681 ccagggaaat cggtgtgcca ggtctgttcg aagaatccag gtgcgtttgt actgaactct
     1741 tgcgcccctt cggtgcgctt gtgcagtaat cagattgtct caaatcccct caagaatcag
     1801 gcctgatagt ttttgaaggc ggactaatgc tatattgtgc accagatggc tcagcccgca
     1861 cgccataagg cccgggcttt ttgttatgaa catgacaggt tcgtcgcgtt tgccaggttt
     1921 ctataatctg ccactcacca gacggctcga agtcgtggca caggaggtcg gcctgacccc
     1981 ggaggaagcc gctgttatgt ccggtatgag cggcttgtcc tctgacgcgg cagatcacat
     2041 ggtcgagaac gccgtcggcg tgttcggcct gcccctgggc attgccacga actttctggt
     2101 caacgggcgc gaggtgctcg tgccgatggc gatcgaggag ccatccgtca tcgccggtgc
     2161 gtcgtttgcc gcgcgcctga ttcgcgaagg cggcgggttc acggccacga gcgacgagcc
     2221 ggtcatgatc gcgcaggtgc agattctcga cctggtgaat ccgcacgcgg ctcgactcgc
     2281 cctgctggcg aacaagcagc gcctgctcga cttcgccaac acgaccgacc cggtgattac
     2341 cggcctgggc ggcggcgcgc gcgacatcga ggcgcgcgtc gtcgagcagg cgccggtcgg
     2401 tccgatgctg gtgatgcacc tgctgtacga ctgccgcgac gcgatgggcg cgaacacagt
     2461 gaacaccgcc gccgaagcgc tcaggccgat gatcgaagag atcaccggcg ggcgcgtcgg
     2521 cctgcgcatc ttaagcaact tggccgatcg ccgcctggcg cgcgccatgg cgaaggttcc
     2581 gcctgcagcc ctggcttttg acgagtacga cggcgaagtc gtgcgtgacc gcatcgtcga
     2641 ggcgtgggcg ttcgccgccg ccgatccgta tcgggcggcg acgcacaaca agggcatcat
     2701 gaacggcgtc gacgcggtcg tcatcgccac cggcaacgac tggcgcgcga tcgaggccgg
     2761 cgcgcatgct tacgcggccc gatccggccg gtacacctcc ttgacgcact gggggcgcga
     2821 cgccgaaggc aacctggtcg gcacgctcga aatgccgatg gccgtcggca tcgtcggcgg
     2881 cgcgacgaaa gtgcatccgg cggcgcgcgg cgcgctgaag atcgtgggca tccggacggc
     2941 gcgcgagttg gccgagatca tcgtgtcggt gggactggcg cagaacctgg cggcgatccg
     3001 ggcgctggcg acggaaggca tccagcgcgg ccacatgacg ctgcacgcgc ggcagctcgc
     3061 ctcggcggcc ggcgcgaccg gcgacctcat cgagcgcatc gcggcgcaga tggtcgccga
     3121 gaagtcgatc cgcctggatc gtgcagaggc gctggtcaga gagattgcag agacagtgga
     3181 gcgtgcgaag tcggtaatca gtgaccagta atcagtgatc agtaatcagt gatcagtaat
     3241 cagtggtcag tgatcagttc cccgtgatca ctgttcactg ataaccggta accgatgact
     3301 gataactgat gactgatgac tgataactga caactgatga gggattagag acgatgggat
     3361 tgatgaaacc gaatcgaccc gtgggcatcg ccggctacgg cgcttacgtg ccgcgctatc
     3421 ggctgccggc gagcgaggtc tctcgggtgt ggacggacgg cacggaaggc acgccgatcg
     3481 cggagaaagc cgtgccgggt ctcgacgaag acgtggcgac gatgagcatc gaagcagcgc
     3541 gcaacgcgct ggcgcgcgct gccatcgacg ccgagcagat cggcgccgta tgggtcggca
     3601 gcgaatcgca tccgtatgcc gtcaagccga cgtcgacgat cgtcgccgaa gcgatcggcg
     3661 cgattcccaa cacgcaagcc gccgactggc agttcgcctg caaggccggc accgaggcat
     3721 tgcaggcggc gatcggattc gtcgcgtccg gcatggcgcg gtacgcgctg agcatcggca
     3781 tggacacggc gcagggccgc cccggcgacg cgctcgaata cacggcgggc gcgggcggcg
     3841 cagcggtcat cgtcggtccg gcggaagagg gcctggccga aatcgaggcg tcgctctcgt
     3901 tcgtcaccga tacgcccgac ttctggcggc ggccatacgc gcactatccc gagcacgcca
     3961 gccgcttcac cggcgagccg gcgtacttca agcacatcac ctcggcgggc gagcagttga
     4021 tgcaggagct tgccacacgc ccggaagact acacctacgc cgtgttccac cagccaaacg
     4081 tgaaattccc gcagcgcgcc gcgcagatgc taggcttcaa gcccgagcag ttcgaggcgg
     4141 gcctgctggt caacgtgatc ggcaacacgt acgccgggtc gtgcatgatc ggcttgacgg
     4201 cgatcctgga ccaggcgaag ccgggcgatc gcatcttcat ggtgtcgttc ggctcgggcg
     4261 caggcagcga cgcgttcagc atcaccgtca cggacaagat cgccggccgg caagagcgcg
     4321 cgacggcgac acgcgactac atcgcgcgcc gcgtgctgat cgactacgcg acgtatgcac
     4381 gctaccgaaa gaaactgctg atgcagtgat cagtcaccag taatcagtga ccagtaatca
     4441 gtgaccggta acgactgacg actgataact gacgactgat aactggcaac tgatgactgg
     4501 caactgacaa ctggattcac tatgcgagac gtaagcatta tcggaatcgg acagacgcgg
     4561 gtgggcgagc actgggacac ttcgctgcgc cacctggcgc tcgaggcaat caagggcgcg
     4621 gcgctcgagg cgaacgtcga tcgcgtcgac ggcctgttcg tcggcaatat gctggccggc
     4681 gagctgagcg gccagggcaa cctcggcgcg ctgatcgccg acttcgtcgg gctgcgcggc
     4741 gtcgaggccg tcacggtcga agcggcatcg gcctccggcg cggccgccgt gcggcaggcg
     4801 tacctggccg tcgccggcgg gctgatggac ttcgcgctcg tcgtcggcgt ggagaagctg
     4861 agcgacgtgg tcggctcgcc ggcggtcacg gctcaggcga cggccaccga cagtgactac
     4921 gaggcggcgc acggcgcgac gccgctggcg atcgcggcga tgatgatgcg ccgctacatg
     4981 cacgagtacg atgcgtcgct ggccgacttc gccgcgttca gcgtcaacgc gcacctcaac
     5041 gcgagcacca acccgtacgc catgttccac aacaagctgt cggtgcagtc gttcgtcaaa
     5101 gcgccgatgg tcgccgaccc gatcaacctg ttcgacgcgg cggccccggc cgacggcgcg
     5161 gcggcgctca ttctggtgcg ctccgaatgg gccaggggac gcggcaacgg cgctgcgcgc
     5221 gtccgcattg cagcctcggc ggctgcgaca gacgcgctcg ccatcgccga cagatcggat
     5281 ccgctgtggc tggcggctgc ggaagcatcg gcgaagcgcg cctaccacca ggcgggcgtc
     5341 acggctcgcg acatccagtt cgcagagctg cacgacgcgg cgacggtgct ggccgccctg
     5401 tcgctcgaag cgtgtggctt cgccgggcgc ggcggcggcg tgaatttcgc caagggaggc
     5461 ggcatcgccc gcggcgggca gacgcccacc ggcacgctgg gcgggctgaa gggccgaggc
     5521 gatcccggcg gcgcgacggg tgtgtaccag atcgtcgagg ccgtgcagca gttgcgcggc
     5581 caggccggcc ccaaccagat cgccgacgcg cgcctcgggc tggcgcagtc gctcggcgcg
     5641 aacggcgc
//
LOCUS       DBNHXB010000492         4754 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_666653, whole genome shotgun sequence.
ACCESSION   DBNHXB010000492 DBNHXB010000000
VERSION     DBNHXB010000492.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_666653"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="AC162G_19005"
     CDS             <1..371
                     /locus_tag="AC162G_19005"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5077950.1"
                     /translation="CGYHCPVGIQVRDYARQGKTVDDEGCVKCGLCIQKCPRGTLRWD
                     SQPEAPSPHFRADKCDLCRGYGESACVKECPTGAMVRLPADKRLKALNEDLYQEVIGR
                     YFGQAGDGPAGRVPNVTLFE"
     gene            648..1985
                     /locus_tag="AC162G_19010"
     CDS             648..1985
                     /locus_tag="AC162G_19010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5077951.1"
                     /translation="MRRYVIIGNGAAGINAAEELRKRDARASITILTDERYPMYSRPG
                     LAYVLIDEVSDQQAIARKPEWYAAQRIDLVFSRAERIDLDRRCVHAGNGQAFPYDALL
                     IAAGARAVSAPFPGGNLDGVVYLDNMDNALDIIRRARSAQAAVVVGGGITALELAEGL
                     AHHKVETHYFVRKNNLWSALLNDHESRLVERQIVHHSVHIHYGTETAEIVGRDGKVAG
                     VRTTKGEVVECQIVGVAIGVKPNLTLAKGTPLALDRGILTDEYLQTNLPGVYAAGDCA
                     QIYDAWSGEHRCDSLWPTAIASARAAAINMTGARQPYRKGVPFNAALLFGLHLTAIGQ
                     VAAAGRGGEDDAEELSFISRGSSEVWTAKPGGGYTSAWAEDGTSSKRLVIRENTIVGA
                     LLLGNQRLADPLRDLIDRRIDVSSFKPRLMAGGDILAEAVMEAWQGRAYATQA"
     gene            1969..3636
                     /locus_tag="AC162G_19015"
     CDS             1969..3636
                     /locus_tag="AC162G_19015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077952.1"
                     /translation="MRRRLSFISIALAFLVLSVGGFVLVSSAQASLPGDGLYGFKLWL
                     EQARLNAAAGDVMRARLSLEFLETRLDELDQVTGTPNAALALEPLRASLAEAVRAVAS
                     LDADSAGDMRAQLARLALRAADVLDRWQAAGMVDPVLDRLRQLARAIGEMAADPGASA
                     AALLLLLDRLPAADADTAPWPIAARGVELPADLVAAHQPPFSGKHAALTCNTCHDGGK
                     VEGLSAACTACHEDPHQGEFERVGHACTACHTDVAFMPSTFDHARFPDCQSCHLVESP
                     ANHYPGQCSNCHAETAWKPADFNHTGFTDCVSCHANVAPPNHYSGQCSTCHTTTAWKP
                     ANFSHTGFTDCVACQANVAPPNHFPGQCSNCHMTSAWQPTSFNHAGFTDCVACHANRA
                     PANHFPGQCSNCHSTSAWQPASFNHTGFTDCVACHANRAPANHFPGQCSNCHTTSAWQ
                     PANFNHAGFTGCAGCHAPPSNHFPGQCSDCHNTDSWEGATFNHTFPIDHKGANGECTQ
                     CHPGGNTSTWTCAGCHDPDKMNEKHKEIQDYSQNCIACHADGRKPED"
     gene            complement(4002..4616)
                     /locus_tag="AC162G_19020"
     CDS             complement(4002..4616)
                     /locus_tag="AC162G_19020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077953.1"
                     /translation="MWPFLGRLIGFEVHSILKSHRWYLPACGILAVLYLVLPYADARQ
                     TLLPIALIIPLPLIADLGCRESRHRVTGFVFSARWIFELYPLWKWGVGFTISLLATNL
                     MFVRLWFASPETGFAALVSLCLVSALALTVGMITDSGRLFALLYVVFWYLGNFEKDVP
                     AWLDYMSLHHTPTPGVWLGQLSLAVGLIALGAVWMRWKHARVGS"
BASE COUNT          979 a         1530 c         1448 g          797 t
ORIGIN      
        1 tgtgcggcta ccactgcccg gttggcattc aggtgcgcga ctacgcccgc cagggcaaga
       61 cggtagacga cgaaggctgc gtcaagtgcg ggttatgcat ccagaagtgt ccgcgcggca
      121 cgctgcgctg ggactctcaa cccgaagcgc cgtcgccgca cttccgcgcc gacaagtgcg
      181 acctgtgtcg cggctacggc gagagcgcct gcgtgaaaga gtgtcccacc ggcgcgatgg
      241 tgcgcctgcc cgccgacaag cggctcaagg cgctgaacga agacctgtat caagaagtga
      301 tcgggcgcta tttcgggcag gccggcgacg ggcctgccgg gcgcgttccg aacgtgactt
      361 tattcgagtg agaagttgat ccatcagatt tcagacctcc gaagtctcag cagatccaat
      421 tctcagactt cggaagtctc aaggcgtatt ctcgcagtcg atggtggccg gaactctgag
      481 caatttcgtt agcaagatgc cacttgaaga cttccgaagt cttggccgtg gctcaaattt
      541 ggatctaccg agtccccgta aacacccgaa gctgaccgga ggggcagaga cttcagagat
      601 ctggagcgac attcgtgtag attcgtggat caatttctgg ggacactatg cggcgatacg
      661 tcatcatcgg caacggcgcg gcgggcatta acgccgccga agaacttcgc aagcgagacg
      721 cgcgggcgag catcaccatc ctcaccgacg aacgctaccc catgtacagc cggcccggac
      781 tggcttacgt gctcattgac gaggtctccg atcagcaggc gatcgcgcgc aagccggagt
      841 ggtacgcagc acagcgcatt gacctggtct tcagccgcgc cgagcgcata gacctcgaca
      901 ggcgctgcgt ccacgccggg aacgggcaag cgtttccgta cgacgcgctg ctgatcgccg
      961 ctggcgcacg ggccgtgtcc gcgcctttcc ccggcggcaa cctcgacggc gtggtgtacc
     1021 tggacaacat ggataacgcg ctcgacatca ttcgccgcgc ccgttcggcg caggcggcgg
     1081 tcgtcgtcgg gggcggcatc accgcgctcg agctggccga aggactggcg catcacaaag
     1141 tcgagacgca ttacttcgtc cgcaaaaaca acctgtggtc ggcgctgctc aacgatcacg
     1201 agtcgcgcct ggttgagcgg cagatcgtgc atcacagcgt gcacatccac tacggcacgg
     1261 agactgccga gattgtcggc cgggacggga aagtggccgg cgtgcgaacg acaaaaggcg
     1321 aagtcgtcga atgccagatc gtgggcgtgg cgatcggcgt gaagcccaac ctgacgctgg
     1381 cgaaaggcac gccgctcgcg ctcgatcgcg gtatcctcac cgacgagtat ttgcagacga
     1441 acctgcccgg cgtctacgcg gcgggcgact gcgcgcaaat ctacgatgcc tggagcggcg
     1501 agcatcgctg cgacagcctg tggcccacgg cgatcgcctc cgcgcgcgcc gcggcgatca
     1561 acatgaccgg cgcacgccag ccgtatcgca agggcgtgcc gtttaacgcg gcgctgctgt
     1621 tcggcctgca cctgacggcg atcggccagg tcgcggccgc cgggcgcggc ggcgaggatg
     1681 acgccgaaga actctcgttc atctcacgcg gatcgagcga agtatggacc gccaagccgg
     1741 gcggcggcta caccagcgcc tgggccgaag acggcacgtc gtccaagcga ctggtgatcc
     1801 gcgagaatac gatcgtaggc gcgctgctgc tgggcaacca acggctggcc gatccgctgc
     1861 gcgatctgat cgaccggcgc atcgatgttt cgtcgttcaa gccgcgcctg atggccggag
     1921 gcgatattct ggccgaggcg gtcatggaag cctggcaagg gagagcctat gcgacgcagg
     1981 cttagtttca tcagcatcgc gctcgcgttt ctcgtgttga gtgttggagg cttcgtgctg
     2041 gtgtcgtccg cccaggccag cctgcccggc gacggcctgt atggctttaa gctctggctg
     2101 gagcaagcgc gcttgaatgc ggcagccggc gatgtgatgc gtgcccggct cagtctcgag
     2161 ttcctcgaaa cgcgcttgga tgagctggat caagtgacgg gcacgccgaa cgccgcgctc
     2221 gcgctcgaac ctctccgggc ttcgctcgcc gaagcggtgc gcgccgtcgc ctcgctcgac
     2281 gcagactcgg cgggcgatat gcgcgcgcag ctcgcccggc tcgcgctgcg cgccgccgac
     2341 gtgctcgacc ggtggcaggc ggccggcatg gttgatcctg tgctcgatcg tttgcggcag
     2401 ttggcgcgcg cgatcggcga aatggccgcc gaccccggtg catcggcagc ggcgctgctc
     2461 ctgttgctcg accggctgcc cgcagcagat gccgacaccg cgccgtggcc catcgccgcg
     2521 cgcggtgtgg agctgccggc tgatctcgtg gcggcgcacc agccgccgtt cagcggcaaa
     2581 cacgccgcgc tgacgtgcaa cacctgccat gacggcggca aagtggaagg cctgtcggcg
     2641 gcctgcacgg cctgtcacga agacccgcac cagggagagt tcgagcgtgt cgggcacgcc
     2701 tgcaccgcct gccacaccga cgtcgcattt atgccatcga cgttcgatca cgcgcgtttt
     2761 cccgattgcc aatcctgcca cctcgtcgaa tccccggcga atcactatcc gggccagtgc
     2821 agcaactgcc acgccgagac ggcgtggaag cctgcggact tcaatcacac gggcttcacc
     2881 gactgcgtgt cttgccatgc caatgtcgcg ccgcccaatc actactcggg ccagtgcagc
     2941 acctgtcata cgaccacggc ctggaaaccg gccaacttca gtcatacagg attcaccgac
     3001 tgcgtcgcct gccaagcgaa tgttgcgccg cccaatcact tccccggcca atgcagcaac
     3061 tgccacatga ccagcgcctg gcagccgacc agcttcaacc acgccggatt caccgactgc
     3121 gtcgcctgtc atgccaatcg cgcgccagcc aaccacttcc ccggccagtg cagcaattgc
     3181 cattcgacca gcgcctggca gccggccagc ttcaatcaca cgggcttcac cgattgcgtc
     3241 gcctgtcatg ccaatcgtgc gccggccaac cacttcccgg gtcagtgtag caactgtcac
     3301 accacgtccg cctggcagcc ggccaacttc aaccatgccg gattcaccgg ctgcgccggc
     3361 tgccacgcgc cgccgtcgaa tcacttcccc ggccagtgca gcgactgcca caacaccgac
     3421 tcgtgggaag gcgcgacgtt caaccacacg ttcccgatcg accataaggg cgcgaacggc
     3481 gagtgcaccc agtgccatcc cggcgggaac accagcacgt ggacgtgcgc cggttgccat
     3541 gatccggaca agatgaacga gaagcacaaa gagattcaag actacagcca gaactgcatc
     3601 gcctgccacg ccgatggaag gaaaccggag gattagagcg gattccactt tgatttacgc
     3661 cggagtcgag gctttagccg gaaaccgcct aaaggctcga ctccggatgg gcgtacacgc
     3721 aatcggaatc cgctctaaga cttccgacta ttaccaactg cgcgggaaat tgaaagagag
     3781 gcttcagcgg caatgggcgg gagaggtgtg gttaagattc actgcgcgcg gcaggagcga
     3841 attgcctctt gacaacgcta ttcatcagta gccggcctga tggaagtata cgatcaggtc
     3901 tccaatggct caatgccgac cacgttctta ctatgcggtg catagtatag tcgatacgga
     3961 actccggtcg ctaaggcatt gtaatcctgg cgcgaaaccc gctaactgcc taccctggca
     4021 tgtttccaac gcatccatac cgcccccagc gcgatcaagc cgacagctaa agagagctgc
     4081 cccaaccaca cgccgggtgt aggtgtatga tggaggctca tgtaatccag ccaggccggc
     4141 acgtcttttt caaagttgcc caaataccag aacaccacat agagaagcgc aaagagtcgc
     4201 ccgctatcgg tgatcatccc tacggtcagc gccagcgcag agacgaggca caagctgaca
     4261 agtgcggcga agcctgtctc cggtgaggcg aaccagagtc tcacaaacat gaggttcgtc
     4321 gccaataacg agatcgtgaa gcccactccc catttccaga gtgggtacaa ttcaaaaatc
     4381 cagcgggcag aaaacacgaa cccggtgacc cggtgccggc tctcccgaca acccaaatcg
     4441 gcaatgagcg gcaacggtat gatcaaggcg atgggcagga gtgtttgacg cgcatccgcg
     4501 tagggtaaca ccaggtagag aacagccagt atgccgcagg ccggtagata ccatctgtgt
     4561 gatttcaaga tgctgtgaac ctcgaagccg atcaatctgc cgagaaaagg ccacatatac
     4621 gccctagcca ttctatcgcg aaccacgcgt gcccgtcgga tcaaagcggt caaaaagaat
     4681 cactgccaag cccaacgcaa gaaagcccag ggtgaaggtg ggggcgcgcg atgccaacgt
     4741 ctggccgctc aaag
//
LOCUS       DBNHXB010000493         8823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_163748, whole genome shotgun sequence.
ACCESSION   DBNHXB010000493 DBNHXB010000000
VERSION     DBNHXB010000493.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8823
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_163748"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..658
                     /locus_tag="AC162G_19025"
     CDS             188..658
                     /locus_tag="AC162G_19025"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5077954.1"
                     /translation="MKIADCMKRDVVSITASASIGQAAARFATRHIGMLPVVEAAGHL
                     VGVLQLRDLLTLVMPDFVQLVEDFDFVHDFGAVESRQPSPETLARPVTEVMQPPISVE
                     ATCGLLRAFAVLREHDLHDLPVVDASGRLVGIASRVDIGAALLAGWRSAEVDAT"
     gene            655..1944
                     /locus_tag="AC162G_19030"
     CDS             655..1944
                     /locus_tag="AC162G_19030"
                     /inference="COORDINATES: protein motif:HMM:NF014135.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HIR5077955.1"
                     /translation="MIPGIVSVLIFAVSLYLIFSEKLNRTITGIAGAVVMVGAGRMLG
                     FYAEEQAATAVDFNTLGLLLGMMILVALLEPTGFFQYLAVWAGRLSRGRPVRLLVLLG
                     TVTTVLSMFLDNVTTVVLIAPVTILISEILGISAVPLLMAEALLADTGGVATLIGDPP
                     NVLIGSAAGFSFNDFLTHALPIVAVAWLTALILLRYLFRSDLDRQPPNADAVLQLNPA
                     EALDEPKTARKVLLVLAAAVLLFFVHHLLHVSPSFIALGAAAAALVWVRPDIHKTLQR
                     VEWSVLIFFAALFVMVGGLEAAGVLGGLVGLLAHAQGVTPMLFGVIMLWAVAVLSAVV
                     DNVPITIALIPVIEGLGAAGMNIRPLWWALAFGAGFGGNGTIIGSTANIIVASVSERT
                     RTPITSALWIKRGLPVMLATCAVASVLYVLAFPLLSR"
     gene            1947..3692
                     /locus_tag="AC162G_19035"
     CDS             1947..3692
                     /locus_tag="AC162G_19035"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HIR5077956.1"
                     /translation="MAALSTALDHLIALYGDVAGRAAFERLHTILDRYRSLPSPSARG
                     VMGEGDAILITYGDQFRAPGEAPLRTLADFARRHLGGLISTIHILPFYPYSSDDGFSV
                     VDYRAVDPALGSWDDVRRIAQDFRLMFDAVINHVSAKSEWFRRFLQDDPAYRDWFITV
                     DPGTDLSMVVRPRALPLLTPFETPSGIRRVWTTFSADQIDLNYANPGVLLEIVDLLLF
                     YVSQSAQLIRLDAIAYLWKEIGTPCIHLPQTHRVIRLFRAVLDEIAPHVSLVTETNVP
                     HADNVSYFGDGYNEAQMVYNFALPPLVLHTFRTGDASALSRWAASLTTPDGFQKPVGS
                     RTTFFNFLASHDGIGLNPARGTPGVTGMDIDALVEQAQAHGGLVSMKNNRDGTRSPYE
                     LNINYFDALSDPGAAEPIERQVDRFMAAQAIMLSLAGVPGVYVHSLLGSRGWPEGVKQ
                     TGRYRTINRQKFDLARLERELADPDSLRSRVFRRYAELLRARAAHPAFDPYGVQRIVE
                     INPAVFAVLRQGPPDVLCLHNVSAQSQACTIRLDSPVNTATDLLTGRTFESGSGHSFA
                     VNLESYEVLWLSLTR"
     gene            3764..5053
                     /locus_tag="AC162G_19040"
     CDS             3764..5053
                     /locus_tag="AC162G_19040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5077957.1"
                     /translation="MKRIAILHFAGPPTVGGVEWTMLQHARLMADAGYAVRIMAGTGK
                     PFDDRVEVVVVPELGSRHPDVLAVKKELDAGQVTPQFDALRGRMTARLRELLADVDIL
                     IAHNVFTLHKNLALTAALSQLINDRSSGRYLGWHHDFAWTNPLYRDQLHDGEPWDLLR
                     RPWPNVRHVTVSESRRRELAELYGVDESAIDVVPPGIDPAGFFGWQPLTRAIVNRLRL
                     LDADIILLLPARITRRKNIELAIRALAALRQQTRLDARLIVSGPPGAHNPANDTYLQS
                     LLALRHELGIEQSTHFLYELDAATLISDQSPVAGHQSSVISYHLPIPDSVIGDLYTLA
                     DALFFPSLQEGFGIPVLEAGLARLPAFCADIPALRETGDELAHYFDPRAEPAAVAALI
                     ASTLEVDQPGRLRRRVRRDYTWPRIFRERIGPLLEAV"
     gene            5050..6867
                     /locus_tag="AC162G_19045"
     CDS             5050..6867
                     /locus_tag="AC162G_19045"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HIR5077958.1"
                     /translation="MTNRPANTYRIVVPIHDNTASPVLLPLAQALLAERAGEIVLVGA
                     VTVPEGRSLSEGATEAQARRQTLANIKTRHADLPIRVKPRVRVSHAPWREIVGSARRE
                     NADMLLLAWDGDPSARLMGASLEVVLAETPCDIALARIPYGQPARRILLPLRGGPYSD
                     LAVTVALALAEANDGAITVLHAAPAGRVDSPAQAFLPIIRHLPRSRQMKVQGDVAGSI
                     LREAEAHDVIVMGASSRVGEIGPKPLGPLAAQIARSTSGGLIMVKSPTAHAPVEDEIE
                     YRDRALSILTDKWFAENSFDADEFADLSQLVAMKEERGLTISLGLPALNEAATVGKVI
                     RTLQRALMHKVPLLDEIVLIDSCSEDSTRDIARRLGVPVYVHQEILPQYGTYAGKGEA
                     LWKSLYVLRGDLIAWIDTDIVNVHPRFVYGVLGPLLRHDTIQYVKGFYRRPIRVGDKL
                     QAGGGGRVTELVTRPLFNLFFPELSGLIQPLSGEYAGRRSALERLPFFSGYGVETGLL
                     IDILNEFGLPAIAQVDLQERIHRNQSLPALSRMSFAILQVIFRRLEDRHKLTLLEEVN
                     RSMKLIRHAPGHYFLDIEEIGDVERPPMITLPEYVAARK"
     gene            complement(6878..8530)
                     /locus_tag="AC162G_19050"
     CDS             complement(6878..8530)
                     /locus_tag="AC162G_19050"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR5077959.1"
                     /translation="MNARSLVIIAVLVALLASACAAPAATPPAGLPNYNPPPAERQEP
                     PAQPAYPAPAPTQPPALAAPQPPADLERSAGSQAGDILPPWPPYPTYPPYPTPWPERP
                     AYDIGVNPFMDAREDHLSTFAMDVDTASYTKMRDYINSGQLPPANLVRVEEFVNYFNM
                     EYPDPTSGAFSINLEGGPSPFGAEGTWLLRVGIQGRHIAGWERKDALLTYVIDVSGSM
                     QEENRIDMVKTGLSRLVDRLGPNDRVAIVAFSETAWVVLEPTSVENRYRILDAINSLY
                     PMNSTNTEAGLRLGYELAHRNYRDGAINRVILASDGVANVGNTDPNALAQYAQDYYGR
                     NIFLSTVGVGHGNYNDVLLEQLADKGNGVYSFLDSAQAAERIFSQDLEGTLQTIAKDA
                     KIQVDFNPNVVHSYRLLGYENRAVADQDFRNDTVDAGEVGAGHNVTALYEVRLSLEVG
                     GEIAIVRVRYEDPDTHAVAEQSEAIRTDELQRDFSRTSPRFQLAAAVAQYAETLRTSP
                     WTSREQLRRVVDLAWSVQRQLPFDADVAEFAQLVERAASLTD"
BASE COUNT         1417 a         2841 c         2848 g         1717 t
ORIGIN      
        1 atgtacctcg gcgcgatggt cacgctgtac attctgggtt aggtagtttc tgtcggaaaa
       61 ttcagcggag gcggctccca cgccgccgaa ttgccgggcg tgggagcccg gccaacagtt
      121 ttccgacaag aacgaggtac cgggggcgtt cgatcgctca gcacgtcgag gggcgaggag
      181 tacacacatg aagatagctg actgcatgaa acgcgacgtc gtttcgataa ccgcctcggc
      241 cagcatcggc caggcagcgg cgcgcttcgc cacgcgccac atcggcatgc tgcccgtcgt
      301 cgaggccgcc ggccatctgg tgggcgtgct gcaactgcgc gatctgctga ccctggtgat
      361 gcccgatttc gtgcaactgg ttgaggactt cgacttcgtg cacgacttcg gcgcggtaga
      421 gagccggcag ccctcacccg agacgctggc ccgcccggtg acagaagtta tgcagccgcc
      481 gatctccgtc gaggccactt gcggcctgct gcgcgcgttt gccgtgctgc gcgagcatga
      541 tctgcacgat ctgccggtgg tggatgcttc gggccgactc gtgggtattg cgtcgcgtgt
      601 ggacattggc gcggcgctgc tggccggctg gcgctccgcg gaggtcgatg cgacatgatc
      661 cccggcatcg tctccgtgct gatcttcgcc gtcagcctgt acctgatctt ctccgagaag
      721 ctcaatcgga cgattacagg tatcgcgggc gctgtggtga tggtcggcgc gggccggatg
      781 ttgggcttct atgccgagga gcaagccgcc acggccgttg acttcaacac actcggctta
      841 ttgttgggca tgatgatcct cgtagcgctg ttagagccga ccggtttctt tcagtatctg
      901 gccgtgtggg cggggcgcct gtcgcgcggc cgcccggtac gcctgttggt gctgcttggc
      961 acggtaacca cggtgctgtc gatgttcttg gacaacgtca cgacggtggt gttgatcgcg
     1021 ccggtgacaa tcctgatcag cgaaatcctg ggcataagcg ccgtgcccct cctgatggcc
     1081 gaggcgctgc tcgcggacac tggcggcgtg gcgaccctga tcggcgatcc gccaaatgtg
     1141 ctgatcggtt cggcggccgg cttctcgttc aacgactttc tcacccacgc cctgccgatc
     1201 gtcgccgtag cgtggctgac cgcgctgata ttgctgcgct acctcttccg ctccgatctc
     1261 gatcggcagc cgccgaacgc ggatgccgta ctgcaactga acccagccga ggcgctcgac
     1321 gagcccaaga cggcgcgcaa ggtgctcctc gtcctggcgg cggccgtcct cttgttcttc
     1381 gtccatcacc tcctgcacgt cagcccctcg ttcatcgcgc tgggcgcggc ggcagcagca
     1441 ctcgtgtggg tgcgcccaga cattcacaaa acgctccaac gcgtcgaatg gagtgtgctc
     1501 atcttcttcg cggcgctgtt cgtgatggtc ggcggactgg aagccgcggg cgtgctaggc
     1561 ggcctggtcg ggcttctggc ccacgcccag ggcgtgacgc cgatgctgtt cggcgttatt
     1621 atgttgtggg ccgtcgcggt gctgtcggcc gtcgtggaca acgtgccgat caccatcgcg
     1681 ctgattcccg tgatcgaggg attgggcgcg gccggcatga acatccggcc gttgtggtgg
     1741 gcgctggcct tcggcgcggg ctttggcggc aacggcacga tcatcggctc gacggctaac
     1801 atcatcgtcg cttcggtctc cgagcgcacg cgcacgccga tcacctcggc gctgtggatt
     1861 aagcgcggcc tgccggtgat gctggcgacc tgcgccgtgg cgagcgtctt gtacgtatta
     1921 gcattcccat tgctgagtcg ctgaagatgg ccgctctttc gaccgctctc gatcatctca
     1981 tcgcgcttta cggcgatgtg gccggccgcg ccgcattcga gcggttgcac accatcctcg
     2041 atcgataccg ctcgcttccg tctccctccg cgagaggggt gatgggtgag ggcgacgcga
     2101 tcctgatcac ctacggcgat cagttccgcg caccgggcga agcgccgctg cgcacgctcg
     2161 ccgatttcgc gcggcgtcac ctcggcggcc tgatcagcac gatccatatt cttccgttct
     2221 acccttactc gtccgacgat ggcttctcgg tcgtcgacta ccgcgccgtc gatccggcgc
     2281 tcgggtcgtg ggatgacgtg cgccgcatcg cgcaggattt ccgcctgatg ttcgacgccg
     2341 tgatcaacca cgtctctgcg aagagcgagt ggttccggcg gtttctgcaa gacgatccgg
     2401 cgtaccgcga ttggttcatc accgtcgatc ccggaaccga tctgtccatg gtcgtccgcc
     2461 cgcgcgccct gccgctgctc acgcccttcg agacgccttc cggtatccgg cgcgtgtgga
     2521 cgacattcag cgccgatcag atcgatctga attacgccaa ccctggcgtg ctgctggaga
     2581 tcgtcgacct gctcttgttt tacgtgtcgc agagcgcgca actgatccgg ctggacgcca
     2641 tcgcctactt gtggaaggaa atcggcacgc cctgcatcca cctgccgcag acgcatcgcg
     2701 tgattcgact cttccgtgcc gtgctggacg agatcgcgcc gcacgtctcg ctcgtcacgg
     2761 agaccaacgt cccgcacgcc gacaatgtat cgtacttcgg cgatggatac aacgaagcgc
     2821 agatggtgta caacttcgcg ctgccgccac tcgtcctgca cacctttcgt acgggagatg
     2881 cctcggcctt gtcgcgctgg gcggcgagcc ttacaacacc cgacgggttt caaaaacccg
     2941 tcgggtctcg aacgacgttc ttcaacttcc tcgcctcgca cgacggcatc ggcctgaacc
     3001 cggcgcgcgg cacgccgggc gtgaccggca tggacatcga cgctctggtg gagcaggcgc
     3061 aagcccacgg cggcctggtg tcgatgaaga acaatcgcga cggcacgcgc agtccgtacg
     3121 agctgaatat caactacttc gacgcgctct ccgatccggg cgccgcagag ccgattgaga
     3181 ggcaggtgga tcgcttcatg gccgcgcagg cgatcatgct ctcgctcgcc ggcgtgccgg
     3241 gtgtctacgt tcacagcctg ctcggctcgc gcggctggcc ggagggcgtg aaacagacgg
     3301 ggcgctatcg cacgatcaac cggcagaagt tcgatctcgc ccggctggag cgcgagctgg
     3361 ccgatcccga ttcgctgcgc tcgcgcgtct ttcgccgcta tgcggaattg ctgcgcgccc
     3421 gcgccgcgca cccggctttc gatccgtacg gtgtgcaacg gatcgtcgag atcaatcctg
     3481 ccgtgttcgc cgtgctgcgc caaggcccgc cggatgtgct gtgcctgcac aacgtctccg
     3541 cgcagtcgca agcctgcacc attcgactgg actcgcctgt gaacaccgca accgatctgc
     3601 tgaccggtcg aacattcgaa tcgggttcag gccactcgtt cgctgtaaac cttgagtcct
     3661 atgaagttct ttggctgtcg ctgacacgct gattcgttgg tatggggccg gcatctggag
     3721 atgcctccta catccgttga ttcgttgcct atccgttgac accatgaaac gtatcgctat
     3781 cctgcacttc gccgggccgc cgacggtggg cggcgtcgaa tggacgatgc tccagcacgc
     3841 gcggctgatg gctgatgccg gctacgccgt gcgcatcatg gcgggcacgg gcaagccatt
     3901 tgacgatcgc gtcgaggtcg tcgtcgtgcc ggagctaggc tcgcgccatc ccgacgtgct
     3961 cgccgtgaag aaggagctcg acgcgggaca ggtcactcca cagtttgatg ccttgcgcgg
     4021 ccgaatgacg gctcgattgc gcgaattgct cgcggacgtg gacattctga tcgcgcacaa
     4081 cgtcttcacg ctgcacaaaa acctcgccct cactgctgcg ctcagccaac tgatcaacga
     4141 tcgcagcagc gggcgttacc ttggctggca ccacgatttc gcctggacca atccactgta
     4201 tcgagatcaa ctgcacgacg gcgagccgtg ggacttgctg cgccggccgt ggccgaacgt
     4261 caggcacgtg accgtctccg aatcgcggcg gcgcgagctg gcggagctgt acggcgtcga
     4321 cgaatcggcg atcgacgtcg tgccgcccgg cattgatccg gccgggttct tcggttggca
     4381 gccgctcacg cgcgccatcg tcaatcggct caggttgctc gacgccgaca ttatcttgct
     4441 gctgccggcg cgcatcacgc gtcgcaagaa catcgagctg gcgatacggg cgctggccgc
     4501 gctcaggcag caaacccgac tcgacgcgcg gctgatcgtc agcggaccgc ctggcgcgca
     4561 caacccggca aacgatacct acctgcaaag cttgctcgcg ctgcggcacg aactcggcat
     4621 tgagcaatcc acccactttc tctacgagct tgacgccgcc acgctgatca gtgatcagtc
     4681 accagtggcc ggtcatcagt catcagtcat cagttaccac ttaccaatcc ctgactctgt
     4741 tattggcgat ctgtacacgt tggccgacgc actcttcttc cccagcctcc aggaaggctt
     4801 cggcatcccg gtgctcgagg cggggctggc gcggctgcct gctttctgcg ccgacatccc
     4861 ggcgttgcgc gagacgggcg acgagctggc gcactatttc gatccgaggg ccgaacctgc
     4921 ggcggtggcc gcattgatcg caagcacgct cgaagtggat cagcccggcc ggctgcgccg
     4981 tcgcgtccgg cgtgactaca cttggccgcg catctttcgg gaacgcatcg gccccctgct
     5041 ggaggccgta tgaccaatcg acccgcgaac acgtatcgta tcgtggtgcc tatccacgac
     5101 aatacggcct cacctgtgct gctgccgctg gcgcaggcgc tgctggccga gcgagccggc
     5161 gagatcgtcc tggttggggc ggtcacggtg cccgaagggc gcagcctgag cgagggcgcg
     5221 accgaggcgc aggcgcgccg ccagacgctg gcgaacatca agacgcgcca cgccgatctg
     5281 ccgatccgcg tgaagccgcg cgttcgcgtg agccacgcgc cgtggcgcga aatcgtcggc
     5341 agcgcgcgac gcgaaaatgc cgacatgctg ctcctcgcgt gggacggcga tccgtcggcg
     5401 cgcttgatgg gcgcctcgct cgaggtcgtg ctggccgaga cgccgtgcga catcgcgctg
     5461 gcgcgcatac cgtatgggca gcccgcacgg cgtatcctgc tgcccctgcg tggcggtccg
     5521 tactccgatc tggctgtgac tgtagcgttg gcgctcgccg aagcgaatga cggcgcgatc
     5581 accgtgttgc atgctgcacc ggccggtcgc gtcgatagcc cagcccaggc cttcctgcct
     5641 atcatccgtc acctgccgcg cagccgccag atgaaggtgc agggcgacgt cgccggcagc
     5701 attctgcgcg aggccgaagc gcacgacgtg attgtgatgg gcgcatcgtc gcgcgtgggt
     5761 gagattgggc caaagccgct cggcccgctg gccgcacaga tcgcgcgcag cacgtcgggc
     5821 ggcctgatca tggtcaagtc gccgacggcg catgcgcccg tcgaagacga aatcgaatac
     5881 cgcgaccgcg cgctgtcgat ccttaccgac aagtggttcg ccgagaactc gttcgacgcc
     5941 gacgagttcg ccgacctgtc gcagctcgtg gcgatgaagg aggagcgcgg cctgacgatc
     6001 agtctggggc tgccggcctt gaacgaagca gccacggtcg gcaaggtgat ccgcacgctc
     6061 cagcgggcct tgatgcataa ggttccgctg ctggacgaga tcgttttgat cgactcgtgt
     6121 tcggaagata gcacgcgcga cattgcgcgc cggctgggcg tgccggttta cgtccatcag
     6181 gagatcctgc cgcagtatgg gacgtacgcc ggcaagggcg aggcgttatg gaagagcctg
     6241 tatgtgctgc gtggggacct gatcgcctgg atcgacacgg acatcgtcaa cgtccacccg
     6301 cgcttcgtgt acggtgtcct ggggccgctc ctgcgtcacg acacgatcca atacgtcaag
     6361 ggattctatc gcaggccgat ccgtgtcggc gacaagctgc aagccggggg cggcggccgc
     6421 gtgacggagc tggtgacgcg tcccctgttc aacctgttct tccccgagtt gtccggcctg
     6481 attcagccct tgtccggcga atacgccggg cggcgttcgg cgctggagcg cctgccgttc
     6541 ttcagcgggt acggtgtgga gacggggctg ctgatcgaca tcctgaacga gttcgggctg
     6601 cccgccatcg cgcaggtcga tctgcaggag cgcatccatc gcaaccaatc gctgcccgcc
     6661 ctgagccgga tgagctttgc gatcctgcaa gtgatcttcc ggcggctgga agaccggcac
     6721 aagctgacgc tgttagaaga agtgaaccgc agcatgaagc tcatccgcca cgcgcctggt
     6781 cactactttc tcgacattga agagatcggc gacgtcgagc gcccgccgat gatcacgctg
     6841 ccggaatacg tcgccgcgcg caagtgacga tccattctca atcggtcagg cttgccgcgc
     6901 gttcgaccaa ttgagcaaac tcggcgacgt ccgcatcgaa cggcagttgg cgctgcacag
     6961 accaggccag atcgacgacg cggcgcaact gctcacgcga tgtccacggg ctggtgcgga
     7021 gcgtctcggc gtactgcgcg acggccgcgg cgagctggaa gcgcggcgac gtgcggctga
     7081 agtcgcgctg cagctcgtct gtgcggatcg cctcgctctg ctcggcgacg gcgtgcgtgt
     7141 ccgggtcttc gtagcgcacg cgcacgatag cgatctcgcc gccgacctcc agggacaggc
     7201 gcacttcgta cagcgcggtc acgttgtgcc cggcgccgac ttcgccggcg tcgacggtgt
     7261 cgttgcgaaa gtcctggtcg gcgacggcgc ggttctcgta gcccagcagg cggtagctgt
     7321 ggacgacgtt cgggttgaag tcgacctgaa tcttggcatc cttggcgatc gtttgcagcg
     7381 tgccttcgag gtcctggctg aagatgcgct cggcggcctg cgcgctgtcg aggaaggaat
     7441 acacgccgtt gcccttgtcg gcgagctgtt cgagcagcac gtcgttgtag ttgccgtgcc
     7501 cgacgccgac cgtcgacagg aagatgttgc ggccgtagta gtcctgcgcg tactgcgcca
     7561 gcgcgttcgg gtcggtgttt ccgacgttcg ccacgccgtc cgaggccagg atcacgcggt
     7621 tgatcgcgcc gtcgcgatag ttgcggtgcg ccagctcgta gcccagccgc aggccggctt
     7681 cggtattcgt ggaattcatt gggtacaggc tgttgatcgc atcgaggatg cggtagcgat
     7741 tctcgaccga ggtcggctcg agcacgaccc aggccgtctc gctgaaggcg acaatcgcca
     7801 cgcggtcatt cgggccgagc cgatcgacca accggctcag gccggttttc accatgtcga
     7861 tgcggttttc ctcctgcata gaccccgaca cgtcgatcac gtaggtgagc agcgcgtcct
     7921 tgcgctccca accggcgatg tgcctgccct ggatgcccac gcgcagcagc catgtgcctt
     7981 ccgctccgaa cggcgacggc ccgccctcga gattgatgct gaaagcgccg gaagtcggat
     8041 cagggtactc catgttgaag tagttgacga actcctcgac gcgcaccaga ttggcgggcg
     8101 gcagttggcc gctgttgatg tagtcacgca ttttggtgta tgatgcggtg tccacatcca
     8161 tcgcgaaggt cgacagatga tcctcccgtg cgtccatgaa ggggtttaca ccgatgtcgt
     8221 aggccgggcg ctcgggccag ggcgtggggt agggcgggta ggtcgggtag ggcggccagg
     8281 gcggaagtat gtcccctgcc tgggaaccgg cgctgcgttc caggtcggcg gggggctgag
     8341 gggcggccaa cgcggggggc tgggttggcg ctggcgccgg gtaggctggc tgtgccggcg
     8401 gctcctgcct ttccgcaggc ggcggattgt aattgggtag gcccgccggc ggcgtggccg
     8461 ctggcgcagc gcatgcggag gcgagcagcg cgacgagcac agcgatgatg acgagacttc
     8521 gggcgttcat tttctctatc tcctcttgac gcaagtgacg cctggcgcaa ctgccggtgg
     8581 ctttgtaaaa ttgccaggcg ctcaccgttt ctgttttgta gatgccggag cgcttgcgaa
     8641 agttcccttt ttggcatcta accttgcgaa ggttcgcgaa ccctggcaag gttggcgacc
     8701 ggctctaatc tcgttctagc acatctgggc tataattcac gccatcgcat aagcccaatc
     8761 caatctccaa tctccaatct ctaatctcta atctccaatc cctaatctcc aacttccaac
     8821 cgg
//
LOCUS       DBNHXB010000494         4074 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_163845, whole genome shotgun sequence.
ACCESSION   DBNHXB010000494 DBNHXB010000000
VERSION     DBNHXB010000494.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_163845"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            536..772
                     /locus_tag="AC162G_19055"
     CDS             536..772
                     /locus_tag="AC162G_19055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077960.1"
                     /translation="MFNHNDLLIEKLARAHHAERLKEAEISRLPVAQAAKTDLSKHKL
                     ALVLMGALLAALLMAQLAAAAAGAGGGAGRCLLM"
     gene            893..1510
                     /locus_tag="AC162G_19060"
     CDS             893..1510
                     /locus_tag="AC162G_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077961.1"
                     /translation="MHEKPLHGRLLGWQLIMNGWLGGTPQPAAGQGAVAGHGQAAQPI
                     APEANMNARNPLAKTSAARQNRAEPMRYSNNRIEVGNITNATGVAIGPGALASVTQVR
                     NGTSDEVASAFAALRQKVNALPGGPVQVVAKSAVDVLEAEAAKGERAKESNVSRWLDF
                     LAQAAPDVWEVAVDTFLNPIKGLGTAFKKIAERAKAEKNGRRDYS"
     gene            1488..3464
                     /locus_tag="AC162G_19065"
     CDS             1488..3464
                     /locus_tag="AC162G_19065"
                     /inference="COORDINATES: protein motif:HMM:NF044287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5077962.1"
                     /translation="MDGVIIADKNLLDVAAGGTITAGNIDRSVVAIGAGAQVIYTRIE
                     RALTEVEIVEQAEKREHERLAEAVTEYVAGLQSQAEQAQRRGGSGNPFKALLEYDFGD
                     AQLFFGRDAAIGALLERLERGPLAVLHAESGMGKTSLLRAGLAPLLLARGHVPLYVRP
                     YDMPIPLAIKRELIAQLEQVPNLAAASLRDFLRRATGLLGGQRLVILLDQFEDVFTVQ
                     SANARADFVAELAECLNDVLLPVRWVLSLRGEWFGHLSTFQPQVPQPFANQFLLGGLL
                     RDEAVGTITRTMERSTLTIEPEVVEQIVDDLGPDAIAPPQLQVVCHALTESLPPGARR
                     LSMDAYVKIGRSEGILRRYLDDSLSRSLQPADRTPAWQALAAVAEQPASGAAESELVA
                     HLKSYGVGEADARRVLRLLETNRLLRVSGERYRLASAGLLPRIRQWAAERAAAELARA
                     EVARQMRRIRDSALRGLLGGAIGFSLAYLVTYIPQIGHKSFLAYVTFFRILPGALAGC
                     LVILSVDVALASYRGPRRWMCWLVGGLAGAGSFAPAVLLHALQQSLFVFDPLTLLPAA
                     AEGAVWGVVAGVGTVWVMSHKLPLWQTLPGAVLASGLALWLSDQFGHAFGNPPSSGLV
                     LLAGAIMPFFVMAAALMTQVSHAGKSGEVSSGYP"
     gene            3535..>4074
                     /locus_tag="AC162G_19070"
     CDS             3535..>4074
                     /locus_tag="AC162G_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR5077963.1"
                     /translation="MSLVISEVLHGKSAETLESLPDTRDLYLGDVLRVRNGGEGLVDF
                     GDSMRLRLFNDTKLGVTKWDIAPDASWQAGMFLTDGGLVGQLVESGNEVAFDTPGGAQ
                     ITVLGTEFFVVYDPVSQETLVGNFHGTVDVSSGGVEITLAADMMVRVRAGQPPDPQQP
                     IGMTREEFEQQARALQSAVE"
BASE COUNT          759 a         1206 c         1342 g          767 t
ORIGIN      
        1 gctggtcgct tcagcccgca tcgcttattc ggaactgtag tttgtcgcct tctgtcactg
       61 ccccgacagc ccctggtttc cagccggggg cttgtttttt taccatgatt ggaatgcata
      121 gcgcagccgc ccgccgcagc caaaggtgtt cggacgcaga tgcgaaacgt gcagatttcg
      181 ctgattttga tcttgtaaac gttcatctgc gaaatatgcg tccgaaaaat cttcgcgatc
      241 gacgtttatg ttgtagagga atactataaa aaccggctga tcgccagagg ctgttcgcat
      301 ctcaatgcgt gaaaaaccgc tccacggtcg tttattaggg tagcagcggc tggagcgatc
      361 caccggatca ggcgcgtgaa ggatcgaggg ctgtgagttg agcctggcgg cgtgctgaag
      421 attgcgggga cgagattgaa ggcgatattg gctgctacgt atcggtggtg agacttgacc
      481 tggaagctct ctccttgaag agagcctatg ggaagatcac cgaagggaag tcaagatgtt
      541 taatcataat gacctactca tcgagaaact tgccagggcg catcatgctg aacgattgaa
      601 agaggcagag atcagtcggc tgcctgtcgc ccaagccgcc aagacggacc tgtcgaagca
      661 caagctggcc ctggtactga tgggcgccct gctggccgcg ctgctgatgg cccaactggc
      721 cgccgccgct gccggcgctg gtgggggcgc tggccgctgc ctgctaatgt aaatacaaca
      781 gacctggcag ccattccgta aatgccctcg gcctccaaga ccgagggcat tccattttgt
      841 ccagcatttt cctagatttt ggtgcctctc gccagaaact gcgcgcatct caatgcatga
      901 aaaaccgctc cacggtcgtt tattaggttg gcaattgatt atgaacggat ggttgggtgg
      961 gacgccccaa ccggcagcag ggcaaggcgc cgtcgccggc catgggcagg ccgctcagcc
     1021 aatcgcaccg gaggcgaaca tgaatgcacg taacccgttg gcaaagacgt cagccgcccg
     1081 acagaacagg gcagaaccca tgcgctattc gaacaaccgc atcgaagtgg gaaatatcac
     1141 gaatgccacc ggcgtcgcca tcggccctgg cgctctagcg agcgtcacac aagtgcgtaa
     1201 tggaacgagc gatgaagtcg ccagcgcctt tgccgcgctc aggcagaagg tcaatgccct
     1261 gccaggtgga ccggttcagg ttgtggcgaa gagcgcggtc gatgtcctgg aggccgaggc
     1321 cgccaagggt gaacgggcca aggagagcaa cgtgagccgg tggctcgact tcctggccca
     1381 ggccgcgccg gacgtatggg aggtggcggt cgacaccttc ctcaacccga tcaaaggcct
     1441 aggcacagcc ttcaagaaga tcgcagagcg ggcgaaagcg gagaaaaatg gacggcgtga
     1501 ttatagctga caagaatctg cttgatgtcg ccgccggggg cacgatcact gccgggaata
     1561 tagaccgcag cgtcgtggcg attggagccg gcgcgcaagt catctacacc cgtatcgaac
     1621 gggcgctcac cgaagtagag atcgtcgagc aggccgagaa gcgtgagcac gagcgcctgg
     1681 ccgaggccgt gaccgaatac gtcgcgggcc tgcagagcca ggccgagcag gcgcagcgca
     1741 gaggcggctc aggcaatccc ttcaaggcac tgctcgaata cgacttcggc gacgcgcagc
     1801 tcttcttcgg gcgcgatgcg gcgatcggcg cgcttctaga acgtttggag cgcgggcctc
     1861 tcgccgtctt gcacgccgag agcggaatgg gcaagaccag cctgctgcgg gccgggctgg
     1921 cgccgctgct gctggctcgc ggacacgtcc cgctatacgt tcggccctac gacatgccga
     1981 tcccgctcgc catcaagcgc gagctgatcg cccagttgga acaagtgccg aacctggccg
     2041 ccgcctcact gcgcgacttt ctgcgtcgcg ccacgggcct gcttggaggg cagcgcctgg
     2101 tcatcctgct cgaccagttc gaagacgtgt tcaccgttca aagcgccaac gcgcgcgccg
     2161 atttcgtcgc ggaactggcg gaatgcctga acgacgttct gctgcccgtg cgctgggtct
     2221 tgtcgctgcg aggagaatgg ttcgggcacc tgagcacctt tcagccgcag gtgccgcagc
     2281 cgttcgccaa tcaattcctg ctgggcgggc ttttgcgaga cgaggctgtg ggcacgatca
     2341 cacgcacgat ggagcgctcg acgctaacga ttgagcctga agtggtcgag cagatcgtcg
     2401 acgacctcgg cccggacgcg atcgcgccgc cgcaattaca ggtggtctgt cacgcgctga
     2461 cggaaagttt gccgcccggc gcccggcgct tgagcatgga cgcctacgtg aagatcgggc
     2521 gcagcgaggg catcttgcgg cgctacctgg acgattcgct gtcacgcagc ctgcagcccg
     2581 ccgatcgcac gccggcatgg caggcgctgg ccgcggtcgc cgagcagccc gcgagcggcg
     2641 ccgcagaatc ggagctggtg gcgcatctga agagctacgg cgtgggcgag gctgacgcac
     2701 gccgcgtgct ccgcctgctc gaaacgaatc gcctgctgcg cgtgagcggc gagcgatatc
     2761 ggctggcgag cgccggccta ctgccgcgca tccggcagtg ggccgccgag cgcgccgcgg
     2821 ccgagctggc gcgcgccgaa gtcgcgcgtc agatgaggcg cattcgcgac tcggcgctgc
     2881 gtggactctt gggcggagcc atcggattca gcctcgccta cctggtcacg tacatcccgc
     2941 aaatcggcca caagtcattt ctcgcctacg tcacgttttt ccgcatattg ccgggagccc
     3001 tcgccgggtg cctggtcatc ctgtccgttg acgtagcgct ggccagctat cgggggccgc
     3061 gccgctggat gtgctggttg gtgggcggcc tagccggcgc aggctccttc gctccggcag
     3121 tgctgctcca tgccctgcag cagtcccttt tcgttttcga tccgctgacg ttacttccgg
     3181 cggctgcgga aggcgcggtg tggggtgttg tggctggcgt aggtaccgtg tgggtcatga
     3241 gtcacaagct accgctgtgg caaacgctgc cgggggccgt tttggccagc gggctggcgt
     3301 tgtggctgtc cgaccagttc gggcatgctt ttggcaaccc gccatcttcg gggctggttt
     3361 tgctggcggg cgcgatcatg ccgttctttg tcatggcggc ggctctgatg acacaggtgt
     3421 cgcacgccgg aaagtcaggt gaggtcagca gtggatatcc gtaatgcttt acttatcgct
     3481 ttgattctag tgctgccagc atgcaacagc gccggcggca caccgattgg cacggtgagt
     3541 ctcgtcatat ccgaagtgct gcacggcaaa agcgccgaaa cgctggaatc gctaccggat
     3601 acccgtgacc tgtatctggg cgacgtcttg cgggtgcgca acggtggcga gggcctggtg
     3661 gactttggcg acagcatgcg gctacgccta ttcaatgaca caaagcttgg agtgaccaag
     3721 tgggatatcg cgcctgatgc ctcatggcaa gcgggcatgt ttcttacaga cggcggcctc
     3781 gttgggcaac tcgttgagtc aggcaacgag gtggctttcg acacgccggg cggcgctcag
     3841 atcacggtgc tcggcaccga attcttcgtg gtgtacgacc cggtcagcca ggagacgctg
     3901 gtgggcaact tccatggcac ggtggacgtg tcgagcggag gcgttgagat cacgctggcg
     3961 gcggatatga tggtgcgcgt gcgggcaggc cagccgcccg acccgcaaca gccgatcggg
     4021 atgacgcgcg aggagttcga gcaacaggcg cgcgctctcc aatcggctgt ggaa
//
LOCUS       DBNHXB010000495        10676 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_500218, whole genome shotgun sequence.
ACCESSION   DBNHXB010000495 DBNHXB010000000
VERSION     DBNHXB010000495.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10676
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_500218"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="AC162G_19075"
     CDS             complement(<1..1292)
                     /locus_tag="AC162G_19075"
                     /inference="COORDINATES: protein motif:HMM:NF013179.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR5077964.1"
                     /translation="MQSSNAHIDLARLKGVWIGFTVDGRLGPDVDPLVRSSWQRCAPR
                     LNPTTFPQWAYLSSDVLRLTLSQHASLTAMARPIMEDVHQFVEGTGVILVLVDSTACV
                     LEMVGDKSMLQIAAALGLRRAAFLDESRIGTNAFASALIEGCPAQTVGPEHYLRTLHE
                     IGSAAAPIYAVDGRLVGSIGLMAQVEQYSYHLLGIAVAASRAIENQLQADMFVHEANA
                     HASELNATVEAISDGVLAWSADGRIMHMNQRATQMLGLSQTEAVGRPLAEYVTLPEGL
                     AKAVARGEELSDVETHFSVGGTQRDCLVSLRPVRDLEGEPTAYIATLRRLDQVRQLVT
                     RQVGAQARLTLDDIPGASTAMQRIRRQAQAAAAAKACVLVLGESGTGKNVLARAIHNC
                     SRRADGPFLAINCRAIPRPLALGEFLGYEAGAYGTASGG"
     gene            1602..2636
                     /locus_tag="AC162G_19080"
     CDS             1602..2636
                     /locus_tag="AC162G_19080"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5077965.1"
                     /translation="MPKLLIADDLIDIRRLLRAWAEVEGYQPLEAGNGMQAVELALSE
                     KPEVIIMDIMMPGKDGLTAIKEIREVDPTVGIVVLTAYGTEQRAVQAMRVGADDYMHK
                     PFDASELRVKLASVLQKQKLRIENAQLQTRLKSILDHYLPRSVVEYMIGSPDLPHLGG
                     ERQTISILFADIRGFSIYAEQTDPEVLIATVNRYLGVATDAVLAQGGTLDKYLGDGLL
                     AMFNSPVKYEDHAWRAARAAYALDRNVDALNTGPDGQGLRFGVGVHTGEAIVGNIGTP
                     ELMSFTAVGESVNLAKQIEDVTGPGQVTLSRATVDAIGAHRLELKKRAPMTVRGRTEP
                     AEVYELLGFK"
     gene            complement(2639..3655)
                     /locus_tag="AC162G_19085"
     CDS             complement(2639..3655)
                     /locus_tag="AC162G_19085"
                     /inference="COORDINATES: protein motif:HMM:NF012945.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR5077966.1"
                     /translation="MQFDLCLLGFGNVGRELVKLIERKREELKARYDLELRIVGIATG
                     RHGIVMNRDGIDARAALDDWDRLLSASPIVSDALKLIRECGAQATIEMTPIDRDTGRP
                     AIDHIEAALSAGQHAISANKGPLAHAYRRLRDLAARKRRAFLFEATVMDGFPIFNLYR
                     ECLPATNVLGFRGVLNSTTNLILTKMEEGMSFEDAVRHAQDIGIAETDPSNDIDGWDS
                     AVKVSVLCNVMMNADVRPQQVERAGIRNVTIERIRDAHSRGRKIRLMCSAERREGRLV
                     TQVAPEEVGPDDIAYRLHGTTSTLSIRTDTLNQLTLLETAPTPTQTAFGVLADIIAAA
                     RGHF"
     gene            complement(3735..4916)
                     /locus_tag="AC162G_19090"
     CDS             complement(3735..4916)
                     /locus_tag="AC162G_19090"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HIR5077967.1"
                     /translation="MRLTDSQKDQVIALTRELVRAKSLPGQERDAAVICERWMKELGY
                     HEVWVDPYGSVVGRRQGAGAGSTIHFDGHTDVVPVTSPEKWRRDPFGSELSDGRIWGR
                     GSSDMKGPLAAMICAGALVPREQFDGTIAVSASVGEETLEGPALAAILRENPADVVII
                     GESSELKIGIAQKGRAGIQIETRGVPAHSSRPEEGINAVYRMMEVVERVRAMPVPTDP
                     VLTPGISELVEIVSSPYPGTSIVPDGCRIRIDRRLVRGETRESVLSTFRDALRGIENV
                     EVRYLRVTLPCYTGEVLEMEDFHPAWVTPRDSGLVKCAEAALSRAGLPIGYFASRYCA
                     NGSASACEMGIPTLIFGPSTPAVAHTIDEYIEVEELLHGTEAYAALAIEVLRHEGDNF
                     A"
     gene            complement(4943..6154)
                     /locus_tag="AC162G_19095"
     CDS             complement(4943..6154)
                     /locus_tag="AC162G_19095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002882.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR5077968.1"
                     /translation="MEQSPLPQRSILHIDLDAFFVSVELLDRPDLRGKPVAVGGRPEE
                     RGVVSSASYEARAFGVRSAMPMRTALQKCPDLIILPARHRVYDEWSRKVMALFYEVTP
                     QVEPVSIDEAYLDVTGAELLWGSPEQIAQTLRARIREQFNLPCSIGVAANKLVAKTAT
                     ERAKPDGLCVVPPGEEAAFLAPMPVERLIGVGPKSAAALRAMGIETIGQLAGAPRERL
                     ARRFGANHADDMSRRAQGISDSPVAVERESKQISQEITFTRDVSDKERLRRVLLELSE
                     GVGARLRAEAVDARTVAIKLRYADFTTLTRQTTLPAPTHLNETIYATAWSLFTKTWTR
                     RPVRLLGVAARNLGTSARQLSLFEPAEDRRERLTQAMDAIRARHGDDALKRASLVKTS
                     KGKRKTSNSKM"
     gene            complement(6211..6642)
                     /locus_tag="AC162G_19100"
     CDS             complement(6211..6642)
                     /locus_tag="AC162G_19100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077969.1"
                     /translation="MSRITHVDGTPASQRNRLRRIIAEILRRLSAKKTIDAETKDMVA
                     FIAIALRLIDDLNEKSCAAWEKRDYFLKADQFRREWAWLAPTADRIEDLVLSEEWSLL
                     PTELAGLSVRFSDVKVNQMTKPASLWQGAHQKLISGKNGRR"
     gene            6813..7316
                     /locus_tag="AC162G_19105"
     CDS             6813..7316
                     /locus_tag="AC162G_19105"
                     /inference="COORDINATES: protein motif:HMM:NF024784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR5077970.1"
                     /translation="MAKRIPLSQGYRRKKRNEGLFAIVDDEDYDWLNQWRWCAIHTGR
                     RSGGYAMRMARDESTGKQKSILMHRLILNAPEDMLVDHVNGNSLDNRRSNLRLATFKQ
                     DRANRRLFKNSRSGFKGVHFDKDLGRWKLTVSAHFDTAEEAAHMYDRIVRMLHGEFAK
                     TNFNVES"
     gene            7389..8444
                     /locus_tag="AC162G_19110"
     CDS             7389..8444
                     /locus_tag="AC162G_19110"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR5077971.1"
                     /translation="MIHAAQSDSRTPSAPPGQPPRHSASEIAFADAFIATLASSPNTV
                     TSYRYGLGRLAEFISHTKYIDPPSPYQVRHFKDDLLLKFHEWMARRGYSAQTRTNYLA
                     VTKRFLIWLDANDRLPAGFQVGKAVNRLKAARGARRSAKAGREPDPGIPRIVTFYDSI
                     PLPFSDSPRDRLERLKLLRARAIVHTLYASAGRVSEVASLTREAVLDGRLSEVRITGK
                     GGKDRVLLLTKDAQQAIAAYCRERHDDFPGLFISHGRGAGRPLGRGTLWSVVKHAAKA
                     LGLYKGTSPHAFRHYRAQQLLHEGMDLDVLQAYLGHADISTTRRIYAPYTALEKVRDQ
                     LETFGRSAKEAADEETA"
     gene            complement(8462..9034)
                     /locus_tag="AC162G_19115"
     CDS             complement(8462..9034)
                     /locus_tag="AC162G_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HIR5077972.1"
                     /translation="MSIDLFAWVILPLFIFFARIVDVSLGTLRIVFVARGRRLQSALL
                     GFVEILIWIVVVGQVIQNLNTPIAYVAYAGGFAAGTYIGLYIEDKLAIGMLAVRIIVA
                     TDAQELLDRLSGANFGVTSVDARGATGEVKIVYTIIMRKDLPAVTRMVDEVSPKAFVS
                     VEEVRSAREGVFPPAAGFRGLRLFARRKGK"
     gene            complement(9063..10238)
                     /locus_tag="AC162G_19120"
     CDS             complement(9063..10238)
                     /locus_tag="AC162G_19120"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR5077973.1"
                     /translation="MQYDFDTPPDRRHTDSLKWSRYDADVLPMWIADMDFPSPASIVQ
                     ALRERIDHGVFGYPAGIMGEPNELPEFRRLIVDRLVDRYGWQVQPEDLVFIPGVVVAS
                     NLACHAVATHNGGVLVQTPVYPPILDAPQKANMRRHDAMLARNSDGAYEIDWDVFGSA
                     ITSETRLFILCNPHNPVGRVFRPDELERMAEICLKRGVVICSDEIHCDLLYTGQRHIP
                     IAALDPEIARNTITLIAPTKTFNLPGLQCSIAIVQNPELRQKYQEARKGLVPWVNVMG
                     LLAAQVAYREGQDWLDQLLAYLEGNRDRLYGYVQSELPGITMARPEATYLAWLDCRQA
                     GIGGNPYEFFLQKARVALADGAMFGPGGEGFLRLNFGCSRALLAEALERMKRALASS"
BASE COUNT         1996 a         3353 c         3327 g         2000 t
ORIGIN      
        1 ccgcccgagg ccgtgccgta ggcgccggct tcgtaaccca ggaactcgcc cagcgccagt
       61 gggcggggaa tcgcgcgaca gttgatcgcc aggaacgggc catcggcgcg gcggctgcag
      121 ttgtgaatgg cgcgcgcgag aacgttcttg ccggtcccgc tttcgcccag caccaggaca
      181 cacgctttcg cggcagcggc tgcctgcgcc tggcgccgga tgcgctgcat cgcagtgctg
      241 gcgccaggaa tgtcgtcgag cgtgagtcgc gcttgcgctc caacctgccg ggtgacgagc
      301 tgacgcacct ggtccagccg gcgcagcgtc gcgatatatg ccgtcggctc tccttccagg
      361 tcccgcaccg ggcgcaggct gaccaggcag tcgcgctggg ttccgccgac gctgaaatgg
      421 gtctcgacgt cgctcagctc ctcgccgcgc gcgacggctt tggccaatcc ttcgggcagc
      481 gtcacatact ccgccagtgg gcggccgacg gcttcggtct gtgacaggcc gagcatttgt
      541 gtggcccgtt ggttcatgtg catgatcctg ccgtccgcgc tccaggcgag cacgccgtcg
      601 gaaatggctt ccaccgtggc gttcagctcg ctggcgtgcg cgttggcctc gtgaacgaac
      661 atgtccgctt gcaactggtt ctcgatcgcg cgggatgcgg ccacggcgat cccgagcaaa
      721 tggtagctgt attgctcgac ctgcgccatc agcccgatcg acccgaccaa ccggccgtcg
      781 acggcgtaaa tgggcgctgc ggcgctgccg atctcgtgca gagtcctgag atagtgctcc
      841 gggccgaccg tttgcgcagg acacccttcg atcagggcgg aggcgaatgc attcgtgccg
      901 atccggcttt cgtccaggaa ggcagctcgc cgcagtccca gcgctgcggc aatctgcagc
      961 atgctcttgt cccccaccat ttccagcacg cacgccgtgc tgtcgacgag caccaagatc
     1021 acccccgttc cttcgacgaa ctggtgaacg tcctccatga tcggccgcgc catggccgtg
     1081 agcgaggcat gctggctcag cgtgaggcgg agcacgtcgc tgctaagata ggcccactgc
     1141 ggaaaggtag tcggattcag gcgcggcgcg cagcgctgcc aggaggagcg tacaagcgga
     1201 tcgacatcgg gtcccaggcg gccatccact gtaaagccaa tccacacccc cttcagtcga
     1261 gccaaatcga tgtgagcgtt cgacgattgc atgggtgtcg tctccgattc tcgtaggggc
     1321 gactctggcg gtcgccctgc acaggcgcga ggcctgcccc tacgttagtt cgagatactt
     1381 cgcttggctc agcatgacta tcgaaattcg ataggcggct tgtatagaat agtcgcatcg
     1441 gcggcgaaag tcaaacctga gacagcttgg cctggcggtg acatcgggat ataatgcacc
     1501 tgtcgccacg cgcgaagtcg tgctgcactg gtcatgtgca ggtgggggcg caaccctttc
     1561 cagcgagagt ggattcgatg atcgagcctc aaaagccatc cgtgccaaaa ttgttgatcg
     1621 ccgacgacct gatcgacatt cgccgcttgc tgcgcgcctg ggcggaagtc gaaggatacc
     1681 agccgcttga ggcgggcaac ggcatgcagg cggtggagct ggcgctgagc gagaagcccg
     1741 aagtcatcat catggacatc atgatgccgg ggaaagacgg actgacggcc atcaaggaga
     1801 tccgcgaggt cgaccccacg gtcggtatcg tcgtgctgac tgcctacggc acggagcagc
     1861 gcgcggtgca ggccatgcgc gtcggcgcgg acgactatat gcacaaaccc ttcgacgcgt
     1921 cggagctgcg cgtgaagctg gcgtcggtgc tgcaaaagca gaaactccgc atcgagaacg
     1981 cgcagcttca gacacgcctg aagagcatac tcgaccacta cctgcccagg tcggtcgtcg
     2041 agtacatgat cggttcgccg gacctgccgc acctgggcgg cgaacggcag actatctcga
     2101 tcctgttcgc cgacatccgg ggcttcagca tctacgccga gcaaacggac cccgaagtgc
     2161 tgatcgccac cgtcaatcgc tacctgggcg tggcgaccga cgccgtcctg gcgcagggtg
     2221 gcacgctcga caagtatctc ggcgacgggt tgctggcgat gttcaactcg ccggtcaagt
     2281 acgaggatca cgcctggcgc gccgcgcgtg cggcatatgc cttggaccgg aacgtcgacg
     2341 cgctcaacac cggcccggac gggcagggac ttcgcttcgg cgtcggcgtc catacgggcg
     2401 aggcgatcgt gggcaacatc ggcacgccgg agttgatgag cttcacggca gtcggcgaaa
     2461 gcgtcaatct cgccaagcaa attgaggacg tgaccggccc cggccaggtg acgctcagcc
     2521 gcgcgacagt ggacgccatc ggcgcgcacc gtctggagtt gaagaaacgc gcgcccatga
     2581 ccgtgagagg tcgcactgag cctgccgagg tgtacgagtt actgggcttc aagtgaagtt
     2641 agaagtgtcc ccgagcggcg gcgatgatgt cggccagcac gccgaaggct gtctgcgtag
     2701 gagtaggcgc ggtttcgaga agggtgagct gattgagtgt gtctgtccgg atgctgagcg
     2761 tgctcgtagt gccgtgcaag cggtaggcga tgtcatccgg gccaacttcc tctggcgcga
     2821 cctgggtgac gagtcggccc tcgcgccgtt cggcggaaca catcaaacga atcttgcgcc
     2881 cccggctgtg cgcatcgcgg atcctctcga tcgtcacgtt gcggatgccg gcgcgctcca
     2941 cctgctgcgg gcggacgtcg gcgttcatca tcacgttgca taggacggat actttcacgg
     3001 ccgaatccca cccgtcaatg tcattgcttg ggtccgtctc ggcaatgccg atgtcctgag
     3061 cgtggcgcac ggcatcctcg aatgacattc cctcttccat tttcgtcaag atcaggttcg
     3121 tcgtgctgtt gagcacacct cggaacccca gcacgttcgt cgcgggaaga cactcgcggt
     3181 acaagttgaa tatcgggaag ccgtccatca ccgttgcttc gaataggaat gcgcgccgct
     3241 tgcgcgcggc caggtcgcgc aggcgacgat aggcgtgcgc gagcggtccc ttgttcgcag
     3301 agatggcgtg ctggccggcg ctcaacgccg cctcaatgtg gtcgatcgcc gggcggcccg
     3361 tgtcgcggtc gatcggcgtc atctcaatgg tcgcttgggc gccgcattcc cggatgagct
     3421 taagcgcgtc ggacacaata ggtgatgcgc tcaggagtcg atcccaatcg tcgagcgccg
     3481 cgcgggcgtc gattccgtct cggttcatga cgatgccgtg ccggccggtg gcaatgccga
     3541 cgatgcgcag ctcgaggtca tagcgcgctt tcagctcttc gcgtttgcgc tcgatgagct
     3601 tgaccagctc gcggccgacg ttgccgaagc cgagcaaaca caagtcgaat tgcatgttcc
     3661 ctactcctct tcgcgtagtc acattggtta tctaaacact atcattacga cctattgctt
     3721 caagagcaaa gacactacgc gaaattatcg ccctcgtgac ggagcacctc tatggcaagc
     3781 gcagcatagg cttccgtccc gtgcaaaagt tcctccacct cgatatactc atctatcgta
     3841 tgcgccactg ccggcgtcga cgggccgaaa atcagcgtcg gaatgcccat ctcgcacgcg
     3901 ctcgccgatc catttgcgca gtaccgcgag gcaaagtagc cgatcggcag gccggcccgc
     3961 gagagcgcgg cctcagcaca ctttaccagg ccgctgtcac gtggcgtcac ccaggcagga
     4021 tggaaatcct ccatctcaag cacctcgccg gtatagcagg ggagggtgac gcgcagatag
     4081 cgcacctcga cgttttcgat gccgcgcagc gcgtcgcgaa acgtcgacaa cacgctctcg
     4141 cgcgtctcgc cgcgcaccaa tcgccgatcg atacggatgc ggcaaccgtc cggcacgatc
     4201 gatgtgccag ggtaggggga cgagacgatc tcgaccagct ccgagatacc cggcgtcagg
     4261 acaggatcgg tcggcacagg catggcgcgc acgcgctcga caacctccat catcctgtac
     4321 acggcgttga tgccttcctc cgggcgggac gagtgcgccg gcacgccgcg cgtctcgatc
     4381 tggatgccgg cgcggccttt ttgcgcgatg ccaatcttga gttctgacga ttcgccgatg
     4441 atcaccacgt cggccggatt ctcacgcaat atcgcggcta acgcaggtcc ctccagcgtc
     4501 tcctcgccga cgcttgccga gactgcgatc gtgccatcga actgctcacg tggcacaagc
     4561 gctccagcgc agatcatcgc cgcgagcggg cctttcatgt ccgacgatcc gcgcccccaa
     4621 atcctgccgt ccgatagctc gctaccgaac ggatcgcgac gccacttctc ggggctcgtc
     4681 actgggacga cgtcggtgtg accgtcgaag tgaatggtgc tgccggcgcc ggcgccctgc
     4741 ctgcgcccga cgacgctgcc gtaagggtcg acccacactt cgtggtagcc cagctctttc
     4801 atccagcgct cgcagatgac ggcggcatcg cgttcctgac cgggcaggct ctttgcgcgc
     4861 acgagctcgc gcgtcagcgc gatgacctga tccttctggc tgtctgtcag tcgcattttt
     4921 ctctccgcat cattcgacaa tgtcacattt tggagtttga agtcttgcgt ttccctttgc
     4981 tcgtctttac gagagacgcc cgcttgagcg catcgtcgcc gtgcctggct ctgatagcat
     5041 ccatggcttg ggtcagccgc tcgcgccgat cttcggccgg ctcgaacagg ctcaactggc
     5101 gcgccgatgt tcccaggttc cgcgcggcga cgcccagcag tcgcaccggc cggcgcgtcc
     5161 acgtcttcgt aaagagcgac cacgccgttg cgtagatcgt ctcgttgaga tgcgtcgggg
     5221 cgggcagggt cgtctgccgg gtcagcgtcg tgaaatcggc atagcgcagc ttgattgcga
     5281 cggttcgcgc gtccacagcc tctgcacgca ggcgcgcgcc cacgccctcg ctcagctcga
     5341 gcaggacccg gcgcagccgc tccttatccg acacgtctcg ggtgaaggtg atttcctggc
     5401 tgatctgctt gctctcgcgc tcgacggcga ccggggaatc gctaataccc tgcgcgcggc
     5461 gactcatgtc gtccgcgtgg ttcgcgccga atcggcgcgc gagcctctcg cgcggtgcac
     5521 cggcgagctg gccgatcgtc tcgatgccca tcgcgcggag cgcggcagcg cttttgggac
     5581 cgactccgat caatcgctcg acgggcatgg gcgcgagaaa ggccgcttct tcgcccggag
     5641 gcacgacgca caggccatcc ggtttggcgc gctcggtcgc cgtcttggcg acgagcttgt
     5701 tcgccgccac gccgatcgag caagggagat tgaactgctc gcgtatgcgc gctctcagcg
     5761 tttgcgcgat ctgttccggc gacccccaca gcagttcggc gccggtcacg tcgaggtacg
     5821 cttcgtcgat cgacaccggc tcgacctgag gcgtgacttc gtagaacagc gccatgacct
     5881 tgcgcgacca ctcgtcatac acgcgatggc gcgcgggcag gatgatcagg tcgggacact
     5941 tctgcagcgc agtccgcatc ggcatggcgg agcgcacgcc gaacgcgcgg gcttcgtacg
     6001 aagcgctcga cacgacgccg cgctcttcag ggcgcccacc gaccgccacc ggcttgccgc
     6061 gaagatcggg ccgatccagc agctcgacgg aaacgaaaaa ggcgtccagg tcgatgtgga
     6121 ggatcgagcg ctggggtagg ggggactgtt ccacttgtga cgccgtggat cagacccgtg
     6181 aggtcttggg agacctcacg ggtctcgtga tcagcgccgg ccgttcttgc cggagatgag
     6241 cttctggtga gcgccttgcc agagcgaggc gggcttcgtc atctggttca ccttaacgtc
     6301 cgagaagcgc accgataagc cggccagctc ggtcgggagc agtgaccact cctcgctcaa
     6361 aaccaggtct tcgatgcgat ctgccgtggg ggccagccac gcccactcgc ggcgaaactg
     6421 atcggccttc aggaaatagt ctcgcttctc ccacgctgcg cacgacttct cgttcaggtc
     6481 gtcgatcagc cgcagcgcga tggcgatgaa cgcgaccatg tctttcgtct ccgcgtcgat
     6541 cgtcttcttc gcgctcaggc ggcgcagaat ctcggcgatg atgcgccgca ggcggttgcg
     6601 ctgcgatgca ggcgttccgt cgacgtgagt gattcggctc atatgaaatc ctgtatcctt
     6661 aatgaggtgc tcgtgaaaca taaccatgat agcacggaag ttctaaccag acaaattcgt
     6721 tgaggcgatc gggcacaagg cgcgctctct ttgacattat agcccatttg ttctataatg
     6781 tttccatctg taatgtcctt ttgaggtgat acatggcaaa gcgcatacca ttgtcccaag
     6841 gatatcgtag gaagaaacgg aatgagggac tcttcgccat tgtcgatgat gaggactatg
     6901 attggcttaa ccagtggcgc tggtgtgcga ttcatacggg gcgcagaagc ggcgggtatg
     6961 cgatgaggat ggcgcgagat gagtctactg gcaagcagaa atcaattctc atgcatagac
     7021 tgatattgaa tgcgccagag gatatgttag ttgaccatgt gaacgggaat agtcttgata
     7081 acagaagatc caatctgcga ctggcaacct tcaaacaaga tcgcgcgaat cggagattgt
     7141 tcaaaaacag tagatcaggt ttcaagggcg tgcacttcga taaagacttg ggaagatgga
     7201 aactcacagt gagcgcgcac tttgacactg ccgaagaagc cgcacacatg tatgatcgga
     7261 ttgtgcgcat gttacatggc gaattcgcca aaaccaactt taacgtcgag tcgtgagtat
     7321 gtttttctgg cgtaccactt ttgaacaggt tgcacgttac gttagatact gatataattg
     7381 ctgtagacat gatccatgca gcacagagcg attccagaac tccctctgcc ccaccggggc
     7441 agccaccgag acattcggcc tcagaaatcg cgttcgccga tgcgttcatc gccacactgg
     7501 catccagccc aaatacggta acatcctatc gctatggact gggacggttg gctgagttca
     7561 tttcacatac gaagtacatt gaccctcctt caccgtatca ggtcagacac ttcaaggacg
     7621 acctgttact caagtttcac gaatggatgg cgcgtcgcgg ctactcagcg cagacacgca
     7681 ctaactatct ggctgtgacc aagcgcttcc tcatctggct cgacgcgaac gatcgcctgc
     7741 ccgccggctt ccaggtcgga aaagccgtca accggttgaa agccgcacgc ggcgcgcgcc
     7801 gctcggcgaa ggccgggcgc gagcctgacc caggcatccc acgcattgtc acgttctacg
     7861 actcgatccc cctacccttt tcggattcgc cgcgcgatcg gctcgaacgc ctgaagcttc
     7921 tccgcgcgcg ggcgatcgtc catacgctgt acgcgtcggc ggggcgtgtg agcgaggtcg
     7981 cgtcgctgac gcgcgaggcg gtgcttgacg gtcgtctgag cgaagtgcgt atcacgggca
     8041 aaggcggcaa ggatcgcgtg ctgctgttga cgaaagacgc gcagcaggcg attgcggcat
     8101 actgccgcga gcgccacgac gatttccccg gcctgttcat ctcacacggg cgcggcgcgg
     8161 gaaggccgct gggacgcgga acgttgtggt cggtcgtcaa gcatgcggcg aaggcgctgg
     8221 gactgtacaa aggcacgtcg ccgcacgcgt tccgccatta ccgcgcgcag cagttgctgc
     8281 acgagggcat ggacctcgac gtgctgcaag cctacctcgg ccacgccgac atcagcacga
     8341 cgcggcgcat ctacgcgccg tacaccgcgt tggagaaagt gcgcgaccaa ctggagacgt
     8401 tcggccgcag cgcgaaggag gcggcagacg aggagaccgc gtaagacctg ccaggtcttg
     8461 cctacttgcc ctttcggcgt gcgaagaggc gcaggccgcg aaacccggca gcgggcggaa
     8521 agacgccttc gcgcgccgag cgcacttcct cgaccgacac gaacgccttg gggctgacct
     8581 cgtcgaccat tcgcgtcacg gcgggcaggt ccttgcgcat gatgatcgtg tacacgatct
     8641 tcacctcgcc cgtcgcgcct ctcgcgtcga cgctcgtcac gccgaagttc gcgccggaca
     8701 aacgatcgag cagctcctgc gcgtccgtcg ccacgatgat gcgcacggcc agcatgccga
     8761 tcgcgagctt gtcttcgatg tatagcccga tgtaggtgcc ggcggcaaac ccgccagcgt
     8821 aggccacgta ggcgatcggg gtgttgaggt tctgaatgac ctggccgacg acgacgatcc
     8881 agatcagaat ctcgacgaag cccagcagcg ccgactgaag cctcctgccg cgcgcgacga
     8941 agacgatgcg cagcgtgccc aggctcacgt cgacgatgcg cgcgaagaag atgaatagcg
     9001 gcaggatgac ccacgcgaag agatctatgc tcacgatttg actctttaag acctggcagg
     9061 tcttatgagg aagcgagcgc gcgcttcatg cgctccagcg cctcggccag cagcgcccgc
     9121 gagcatccga agttcagccg caggaatccc tcgccgcccg ggccgaacat cgcgccatcg
     9181 gccagcgcaa cgcgcgcttt ctggaggaag aactcgtagg gattgccgcc aatgccggcc
     9241 tgccggcaat ccagccaggc cagatacgtc gcttccggcc tggccatcgt gattccgggc
     9301 agctcgctct gcacgtagcc gtacagacgg tctcgattcc cctccaggta ggcgagcagt
     9361 tgatccaacc agtcctgtcc ctcgcggtag gcgacctgcg cggcgagcaa gcccatcacg
     9421 ttgacccagg ggaccagccc tttacgcgcc tcctggtact tctgccgcag ctccgggttc
     9481 tgaacgattg cgatcgagca ttgcagccct gggagattga acgtcttggt cggcgcgatc
     9541 agcgtgatcg tgtttcgggc gatctccgga tcgagcgcgg cgatcgggat atgccgctga
     9601 ccggtgtaaa gcaggtcgca gtgaatctcg tccgagcaga tgacgacgcc gcgcttcagg
     9661 cagatctcgg ccatgcgctc cagctcgtcg gggcggaaga cgcggccgac cgggttgtgc
     9721 gggttgcaca gaatgaacag ccgcgtctcg gacgtgatgg ccgacccgaa gacgtcccag
     9781 tcgatctcgt aggccccatc ggaattgcgg gccagcatcg cgtcgtggcg acgcatattc
     9841 gccttctgcg gcgcgtccag gatcggggga tagacaggcg tttggacgag cacgccaccg
     9901 ttatgcgtgg caaccgcgtg gcacgccaga ttggaagcga cgaccacacc cgggataaag
     9961 acgaggtcct ccggctgaac ttgccagcca tatcgatcca cgagccggtc gacgatcagg
    10021 cggcggaatt cgggcagctc gttgggttca cccatgattc cagccgggta gccgaagacg
    10081 ccatgatcga tccgctcgcg cagcgcctga acgatggacg cgggcgacgg gaaatccatg
    10141 tcggcgatcc acatcggcag cacgtctgcg tcataccggc tccacttgag gctgtcggtg
    10201 tggcggcgat cgggaggggt gtcgaagtcg tattgcatgt gcaagtccct atttgtttga
    10261 agcagggcgg gctcccacgc ccgcggatca gcgagcaggt gtcaggcttc cgatgtattt
    10321 tacgagcatt caccccgccg cgcaaaaaga gattgctcac tgcgttcgga ctgacacgct
    10381 tcctcatctg gccaggcgaa cctagaccct tgacgtggca cagtgggtgt tttatagttg
    10441 acccaacgcc atcccctccc ctccccgctc cccgaccgtg ctcgtcgggg gagggcgccg
    10501 aaggcaaggt tttggacttt ggaatttgaa cttaggagtt tcgtatgaac ctcagtcatc
    10561 tggcgcagtc aatcgccacg tcgccgacga tggcgatgaa cgagcaggca cgcctcctgc
    10621 gcgagcgcgg cgaggcggtc atccacctgg gcatcggcga gcctaagaac aaggcg
//
LOCUS       DBNHXB010000496         3077 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_1004510, whole genome shotgun sequence.
ACCESSION   DBNHXB010000496 DBNHXB010000000
VERSION     DBNHXB010000496.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_1004510"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="AC162G_19125"
     CDS             <1..240
                     /locus_tag="AC162G_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HIR5077974.1"
                     /translation="QGRRAPIVGRVCMDQTMIDVTHVPAVAQGDEVVLIGAQGEDRIR
                     AEDVARNLGTNNYEVVSMLMSRVPRLYLRQGSAST"
     gene            385..1068
                     /locus_tag="AC162G_19130"
     CDS             385..1068
                     /locus_tag="AC162G_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR5077975.1"
                     /translation="MGDLEQLIQTVGYLGLFAIVFAESGLFFGFFLPGDSLLLTAGLL
                     ASRTDPSSGQPILNIWVLMPLLAVAAIIGDNVGYWFGAKAGPHIFNRENSRFFKRKNL
                     IAAHNFYEKHGGKTIVLARFMPFIRTFAPIVAGAAYMSYRRFFFFNVLGGLLWAMGVT
                     LAGYGIGKAAANVAPEQVDKYFLILVAVVIFVSALPAMIHVWRENKHEILRRLRRQPA
                     PAPVEAIEE"
     gene            1448..1834
                     /gene="gcvH"
                     /locus_tag="AC162G_19135"
     CDS             1448..1834
                     /gene="gcvH"
                     /locus_tag="AC162G_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR5077976.1"
                     /translation="MNIPGDLKYTRDDEWIRVTGGEGEVGITDYAQDHLSDIVYIELP
                     DAGKVVNSHDSIGTIESVKAAADIYSPVSGTITAVNESLPDSPELVNQDPYGRAWMVR
                     IELSNPSELDALMDAAAYQKYCEERG"
     gene            1886..>3077
                     /gene="gcvPA"
                     /locus_tag="AC162G_19140"
     CDS             1886..>3077
                     /gene="gcvPA"
                     /locus_tag="AC162G_19140"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HIR5077977.1"
                     /translation="MISRVTLTRKESAMTYIPHTDADRTAMLEAIGVTKLEDLFGDVP
                     ERARFPKLDLPPASSEMEAWWEMQGLSEANADVNHYACFLGAGVYNHYSPHTVDYVLR
                     RGELYTAYTPYQPEISQGTLQAMFEYQSMICQLTGMEVSNASHYDGATSLAEGVLLAL
                     NAVKGDRRKVIVSPALNPQYRQVVRTYLQGTGVSVAGDENLMAEMSDLRALLDDQTAA
                     LIIQNPNFFGQFEDVAGLADAVHAAGALFIVVPDPISLGLFQPPGAYGADVVAAEGQC
                     LGIPLSFGGPYLGIFATKNAYVRKMAGRLVGETRDVDGNRGYVLTLSTREQHIRREKA
                     TSNICTNVGLIALAAAVYLATMGRCGLEQVAKLCYHKAHYAAGQIDKLRGYRVNPQAS
                     KPFFK"
BASE COUNT          582 a          956 c          972 g          567 t
ORIGIN      
        1 cagggccggc gcgcgccgat cgtcgggcgc gtgtgcatgg atcagaccat gatcgacgtg
       61 acacacgttc cggcagtggc gcagggcgac gaggtcgtgc tcatcggcgc gcagggtgag
      121 gatcggattc gagcggagga tgtggcacgc aacctcggca cgaacaacta cgaggtcgtg
      181 tccatgctca tgtcgcgcgt gccgcgcctt tatcttcggc aaggctctgc gtcgacatag
      241 gcgccggctt ccccgctgta tccttaccga tttctccttg tccggccagg caaaccgtgg
      301 tattctctaa attggccgcg cataccggac cccgctcgcg tgagcggggt ttcatttggg
      361 gagtccgcgc gcagaggatt cgtgatgggt gatctcgagc aactcattca gaccgtcggt
      421 tacctcggcc tgtttgccat cgtcttcgcc gaatcgggct tgttcttcgg ctttttcttg
      481 ccgggcgaca gcctgctgct caccgccggc ctgctggcgt cgcggaccga tccgagcagc
      541 ggccagccga ttctgaacat ctgggtcctg atgccgcttc tcgccgtggc ggccatcatc
      601 ggcgataacg tgggctactg gttcggcgcc aaggccggcc cgcacatctt caatcgcgaa
      661 aactcgcgct tcttcaagag gaagaatctg attgccgccc acaacttcta cgaaaagcac
      721 ggcggcaaga cgatcgtgct ggcgcgcttc atgcctttca tccgcacctt tgcgccgatc
      781 gtcgcgggcg cggcgtacat gagctaccgc cgcttctttt tcttcaacgt cctcggcggc
      841 ctgctgtggg cgatgggcgt gacgctggcc gggtacggca tcggcaaagc cgccgccaat
      901 gtcgctccgg agcaggtcga caaatacttc ctgattctgg ttgcggtggt gatcttcgtc
      961 tcggcgctcc cggcgatgat ccacgtctgg cgcgagaaca agcacgagat tctgcgccgc
     1021 ctacgccgcc agccggcccc ggcgcctgtc gaggccatcg aagaataagc cgctctccga
     1081 caaaggacag gcgcgaatcg tgacatcttg cggttgacac gcctgaggca cgcggctaca
     1141 atctagccgg ggtaaagcct gctcatcctc tggaaagtcg ccgagccgct tcgccgtgtg
     1201 gccggcgaca ccgaagggcc aactcctcca tgcggcatgg ggacgactct ctcaggtcac
     1261 aggcggggga cgcacagcac agtcgtgctt tgcgtccctt tgttatgggc aggcgccatc
     1321 cagtctcggt cgatccgaaa acgctctgag gggattgccc aatccccggt ttgggcctga
     1381 atttggcaat ccaggccgag caaaatcgga tctgctgagt cttattccaa tcgtcaagga
     1441 gaaaaacatg aacattcccg gcgatctcaa gtacaccagg gacgacgagt ggatccgcgt
     1501 cacaggcggc gagggggagg tcggcatcac cgactacgcg caggatcacc tgagtgacat
     1561 cgtgtacatc gagctgcccg acgcgggtaa agtggtgaac agtcacgaca gcatcggcac
     1621 gatcgaatcg gtcaaggctg ccgccgacat ctattcgccg gtgagcggca cgatcacggc
     1681 cgtgaacgag tcgcttcccg attcgccgga gctggtcaac caggatccgt acggccgggc
     1741 ctggatggtg cgcatcgagt tgtccaatcc gtcggaactg gatgctctga tggatgcggc
     1801 agcgtatcaa aagtattgtg aggagagagg ctaaaacgcc cgcgcctgca gcgcgagcgc
     1861 aggcgtgatg cgtgaagcgt gacgagtgat ttcacgcgtc acgctcactc gaaaggaatc
     1921 ggcgatgacc tatattcccc ataccgacgc cgaccgcaca gccatgctcg aggcgatcgg
     1981 cgtaacgaag ctcgaagacc tgtttggcga cgtgcccgag cgcgcgcgct ttcccaagct
     2041 ggacctgccg ccggcttcct ccgagatgga agcctggtgg gagatgcagg ggctgtcgga
     2101 agcgaacgcc gacgtgaacc actatgcctg ttttctgggc gcgggcgtat acaaccacta
     2161 cagcccgcac accgtcgact acgtgctgcg gcgcggcgaa ttgtacacgg cgtacacgcc
     2221 ttaccagcct gagatcagcc agggcacgct gcaggccatg ttcgaatacc agagcatgat
     2281 ctgtcagctc actggcatgg aggtgagcaa cgccagccac tacgacgggg ccacttcact
     2341 ggccgagggc gtgctgctcg cactcaacgc ggttaagggc gaccgcagga aggtgatcgt
     2401 ctccccggcg ctaaatccac aatacaggca ggtcgtgcgg acgtatctgc aaggaaccgg
     2461 cgtgtcggtc gccggcgacg aaaacctgat ggccgagatg tccgatctga gggccttgct
     2521 cgacgatcag acggccgcgc tgatcattca gaatcccaac ttcttcggac agtttgagga
     2581 cgtcgccggc ctggcggatg ccgtgcatgc tgcgggcgcg ctcttcatcg tcgtgcccga
     2641 tccgatctcg ctcggcctgt tccagcctcc cggcgcgtac ggcgcggacg tcgtcgcggc
     2701 agagggccag tgtctcggca ttccgctctc gttcggaggg ccgtacctcg gcatattcgc
     2761 gacgaagaac gcctacgtgc gcaaaatggc gggccgcctg gtcggcgaga cgcgcgacgt
     2821 ggacggcaat cgcggctacg tgctcacgct cagcacgcgg gagcagcaca tccggcgcga
     2881 gaaggcgacg agcaacatct gcacgaacgt cggcctgatc gcgctggctg cggccgtgta
     2941 cctggcgacg atgggcaggt gcggcctcga gcaggtggcg aaactgtgct atcacaaagc
     3001 ccactacgcc gccgggcaga tcgacaagct cagaggctac cgggtgaatc cgcaggccag
     3061 caagccgttc ttcaagg
//
LOCUS       DBNHXB010000497         8623 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate MAG04708
            SRR25158203_k127_838648, whole genome shotgun sequence.
ACCESSION   DBNHXB010000497 DBNHXB010000000
VERSION     DBNHXB010000497.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414115
            Sequence Read Archive: SRR25158203
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:59:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,837
            CDSs (total)                      :: 3,793
            Genes (coding)                    :: 3,760
            CDSs (with protein)               :: 3,760
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 3 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 5 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8623
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158203_k127_838648"
                     /isolate="MAG04708"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.50166667 N 112.8005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="AC162G_19145"
     CDS             complement(<1..218)
                     /locus_tag="AC162G_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR5077978.1"
                     /translation="MTQPDPQPFMGITTFARQPHTRDARGADVAIVGVPFDSGTSFRS
                     GARFGPRKIREMSLLLWGYNNLLSVAPF"
     gene            349..1182
                     /locus_tag="AC162G_19150"
     CDS             349..1182
                     /locus_tag="AC162G_19150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HIR5077979.1"
                     /translation="MLPFDPDFDDARWTLEWAKVPILQVPIGSHPDGYVVIERHLTQA
                     QIFDRIYRASEPFPVRALLNADGKLWMSDTPQERMMMYNNAMASQGHILIGGMGLAMY
                     PQYAARRATRFTIVEQSGAVVEIVGPTLERALKDLTGLQDPSGLPFDIIVGDVEAFLS
                     AEPAMRFDTIFLDTWDTLAATNLPRINGWRDQAIRHLAPNGRVLLWGYRWMVRLFEDA
                     CRQLLSVPPDQREAWLAQFKQPEAAALLRPVMAKFAGQVVADIGVALRWCREHATLQG
                     T"
     gene            complement(1265..1582)
                     /locus_tag="AC162G_19155"
     CDS             complement(1265..1582)
                     /locus_tag="AC162G_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002793499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HIR5077980.1"
                     /translation="MAIALYMDHHVPRAITIGLRLRGVNVITAYEDGAHRLDDPQLLD
                     RASELGRVLFTQDDDFLTEATRRPREGVPFHGLIYAHQLHVSIGTCVRDLEIIAKAGE
                     PGT"
     gene            complement(1582..1896)
                     /locus_tag="AC162G_19160"
     CDS             complement(1582..1896)
                     /locus_tag="AC162G_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015216420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR5077981.1"
                     /translation="MSMVETRYEHIALNEDNVPFIAGTTMKVIELVLERMAYGWSPEE
                     LHFQHPYLTLGQIHSALAYYWDHQEELDRDIERRLQHVQEVQRATPPSPLDARLKATG
                     LT"
     gene            complement(1973..5506)
                     /gene="mfd"
                     /locus_tag="AC162G_19165"
     CDS             complement(1973..5506)
                     /gene="mfd"
                     /locus_tag="AC162G_19165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR5077982.1"
                     /translation="MRPLAVLRPARPFLIAALTRDLDRPMLYVTARLDRAQIVHEALR
                     AYVGDDAALLRFPEPAALFYERAPWASEVVAERLNVLSTLAADHGVSPTGSAQPSAVG
                     RRPSSLVVVACVRALMFRTLPPRNMALGTRTLKQGQALDLEKVLELWTGLGYAAMTTV
                     IAPGEFSRRGGILDIWPLASPAPVRIELFGDQIESLRRFDPATQRSHQTVEVANITPA
                     SEALARYGPAAADRMRAWSLEHLPDDVEAQLARDRSALATGAPFRGIEFYLPYFYTQS
                     ASLADYLPPDTLIAIDDAGEMEDAWSELEEQAVDLRRSAGEAGTLPPDFPLPYLTWEE
                     WRESLSTRSVISLAEVPPADEGPQPPDHTTIRPPDDAATRLPFVPGPRFGGQLKSFLD
                     HVEQIRRIGDAIVVVSRQAQRLAELWAERDHMRHPISHLQSPPGPRSLEFVHGTLDDG
                     WTLHGVRGRPALSGAEGAASELFSLHVLSDGEIFGWARPQPRKRIRPRAIAPEQFFAD
                     IQPGDYVVHVEHGIGVFQGLVKLTIEGIDGEFLQVAYAGSDKLYVPIHQADRLSKYIG
                     ATDRPPDIHRLGGADWTQVREHAQQAAIEVAKELLELYAARELAPGFAFSADTPWQAE
                     LEAAFPYVETEDQLRAIKEIKADMEKPRPMDRLICGDVGYGKTEVALRAAFKAVQDGK
                     QVAVLVPTTVLAQQHYNTFSSRLAPFPTIVEMLSRFKSPKEQDDILNRLHEGQVDIVI
                     GTHRLLQNDVVFKNLGLLVVDEEQRFGVTHKEMLKRMRAEVDVLTMTATPIPRTLYMS
                     LTGVRDISTIDTPPEERLPVATYVGEYDDHIVRQAILRELDRGGQVYFVHNRVLGIEH
                     IEKRLAALVPEARIGVGHGQMDEHDLEKVMIEFVSREIDVLLCTSIIESGLDIPNANT
                     LIVDRSDFFGLAQLYQLRGRVGRGAQRAYAYFLTDPHSPLTEDARRRLDTIREASELG
                     MGYSIAMRDLEIRGAGDLLGVRQSGQISAVGFDLYTRLLRRAVEEMKAAREGSEPPPA
                     PITGVTLDLPLQAYLPEDYVPNDTLRLQLYRRMGNLASFKEIEELEKELTDRFGALPK
                     PVLNLTFQLRLKVLAAQARVKSVVRDRERNMLVIHCDALEHRDRHSLQRRIGTFAQVA
                     RRQINLPLRDAPRIVGWREALTKVLEEMAES"
     gene            complement(5559..6281)
                     /locus_tag="AC162G_19170"
     CDS             complement(5559..6281)
                     /locus_tag="AC162G_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077983.1"
                     /translation="MKDETLIREFTRIFRCTPEMAPTYLKRVHERMGYEVPLESIVAA
                     LHQISPKRLTMDALIERLRKMERTPRRARKPAPSQGSGQALSLEGSPVAERGDPERSR
                     RTVGAPAGVDPQPALRSPRPRAKRGGAGSRSPGPERSEGEGTAKGSESSKPSAMTQIS
                     IVLTRNWERGRRQGLKPSVMTTDRFVKTAQSIAGVPTPSVARVLKSVEKLDRLDILLT
                     PNLVADDINGSDEPLRSPPSAQ"
     gene            complement(6278..6964)
                     /gene="pth"
                     /locus_tag="AC162G_19175"
     CDS             complement(6278..6964)
                     /gene="pth"
                     /locus_tag="AC162G_19175"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017627072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR5077984.1"
                     /translation="MHLLERLRGKRTPEPALRSPRPKGAGGEGEGSSAPTALIIGLGN
                     PGREYAGNRHNVGWHVADHFVSSRGWRFSKRRNDALIAMGRIGEVRVVVAKPQTFMNL
                     SGRAVQPLARFYKTPPDRMLVIYDDLDLPFGAIRLREKGGAGGHNGMKSIIERMGSGD
                     FPRLRIGVGRPPGRMDAADYVLHDFDASERAELPDVLDRAARAIETFVAHGIGVAMNA
                     HNRTVENASP"
     gene            complement(6979..7698)
                     /locus_tag="AC162G_19180"
     CDS             complement(6979..7698)
                     /locus_tag="AC162G_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077985.1"
                     /translation="MQRYFGGALAALLVAATCVMPVAGAPQAQGDQAVIISPADGQQV
                     SGLVPVFGTATASDFARYELAFGPDPNPSDTWTTFATPDVVLVNAQIGLWDANLPPGL
                     YQLRLRVFRSDGSIAAEDFANGLIVGTPPTETPAPTPTSVPPPPTFEPETQGTIQSTV
                     VIEQPPTPTPAPTLAGGGSAGDASSSRGGGSSIDLSRFGSAFTDGVMCAAGAFLLLGA
                     AWLARWAFRLIMKQMRERSNQ"
     gene            complement(7793..>8623)
                     /locus_tag="AC162G_19185"
     CDS             complement(7793..>8623)
                     /locus_tag="AC162G_19185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5077986.1"
                     /translation="AVFFGVNLNRMNEGVARSPTPPRFNGWALEPAAKVGQAVRDLTE
                     ANRLPRRIVADGQSEILSGMSGVRVKTLSGLDFPNFVVLPGSEPLLYVLVNTSVEPGI
                     LGPHQESFVEYDLQFADGAHTSFVRVQPYDRSAALALPGVKVDWPSESGLSLLGYTLE
                     DNAQPGDGIDVTTYWRVDALQPGREEWFISAFYHLLNAQGQKLAEENGHGQWSRRWEP
                     GDIYVERVSIPVPKDVTLGSYTLEVGLFDSVHARGYSLQSPDGPVYSYSIPIAAGQTD
                     "
BASE COUNT         1501 a         2704 c         2805 g         1613 t
ORIGIN      
        1 ttgaacggcg cgacgctcag caggttgttg tagccccaca gcaggagcga catctcgcgg
       61 atcttgcgcg ggccgaagcg cgcgccgctg cgaaacgacg tgccgctgtc gaagggcacg
      121 ccgacgatgg cgacgtctgc gccgcgcgcg tcgcgcgtgt ggggctgccg ggcgaaggtg
      181 gtgatgccca tgaagggttg agggtcgggt tgggtcatac gacagtgtat cctctggtgc
      241 gtaaaacgta aagcgtaaaa cgtaaagtgt gaaacgtgat ttgtgtggcc tgcaaaggcg
      301 cattatactc cacagcaacc cttacgtttt acgtttcacg tttttgccat gttgccgttc
      361 gatcccgact tcgacgacgc gcgctggacg ctcgaatggg cgaaggttcc cattctgcag
      421 gtgccgatcg gcagccaccc tgatgggtac gttgtcatcg agcgtcacct gactcaggcg
      481 cagatcttcg atcgcatcta tcgagccagc gagccgtttc cggtgcgggc gttactgaat
      541 gccgacggca agctgtggat gtcggacaca ccgcaggaac ggatgatgat gtacaacaac
      601 gcgatggcgt cgcaggggca catcctgatc ggtgggatgg ggctggcgat gtatccgcag
      661 tacgcggccc gccgcgccac gcggttcacg atcgtcgagc agagcggggc cgtcgtcgag
      721 attgtcggac cgacgctgga acgcgccttg aaagacctga cgggtctcca agacccgtca
      781 ggtctgccct tcgatattat cgttggcgat gtcgaagcat tcctctcggc agagccggcg
      841 atgcgcttcg atacgatctt cctcgataca tgggatacgc tggctgcaac gaatttgcca
      901 cggataaacg gatggcgcga tcaggcgatc cgccatctgg ccccgaacgg gcgcgtgcta
      961 ctgtggggtt atcgctggat ggtgcgactg ttcgaggacg cctgccgcca attgctgagc
     1021 gtgccgcccg atcagcgcga agcgtggttg gcgcagttca agcagccgga agcggcagcg
     1081 ttgctgcgtc cagtcatggc gaagttcgct ggacaggtcg tggccgacat aggcgtggca
     1141 ttacggtggt gccgtgaaca cgctaccctg cagggcacct gactgagggt cgccggttaa
     1201 agtgctgatc tgccgcgcgc gacctgggtg attcgctgca gcggcaggaa ccgaacgtga
     1261 cccatcaagt ccctggctcc ccggctttgg caatgatctc aagatcgcgc acgcaagttc
     1321 cgatggaaac gtgaagttga tgcgcgtaga tcaggccgtg aaagggaacc ccctcccttg
     1381 gacgtcgggt ggcctcggtg aggaagtcgt catcttgtgt aaacaagacg cgccctaatt
     1441 cgctggcgcg atccagcagt tgcggatcat ccaggcggtg ggcgccgtcc tcataggcgg
     1501 tgattacatt gacaccacgc aggcgaagtc cgattgtaat agcgcgcggc acatggtgat
     1561 ccatgtagag cgcgatcgcc atcaggtcag ccccgtggcc ttcagccgcg cgtcgagggg
     1621 cgacgggggc gtggcgcgtt gaacttcctg aacatgttga aggcgtcgct cgatgtcccg
     1681 atcaagttcc tcttgatgat cccagtagta ggccaaagcc gagtggattt ggcctagcgt
     1741 cagatacgga tgctgaaaat gcagttcttc tggactccag ccgtaagcca ttctctccag
     1801 gaccagttcg attaccttca tagtggtccc ggcaatgaat ggcacattgt cttcgttcag
     1861 tgcgatgtgt tcatagcgtg tttcgaccat cgacatgtgt gcacctcatt atgcgccctt
     1921 agactatcgt gacctgattc tatctcaaaa ccataccgta tacaatctcc ggtcaactct
     1981 cggccatctc ctccagtacc ttcgtgagag cctcgcgcca gccgacgatg cgcggagcgt
     2041 cccgcagcgg caagttgatc tgccgccgcg ccacttgagc gaaggtgccg atgcgccgct
     2101 gcaagctgtg tcgatcccta tgctccagcg cgtcgcagtg gatgacgagc atgttccgct
     2161 cgcggtcgcg cacgaccgac ttcacccgcg cctgcgccgc cagcaccttg agacgcaact
     2221 ggaacgtcag gttcaacacc ggcttgggca acgccccgaa ccgatcggtg agttcctttt
     2281 cgagttcctc gatttcctta aaagatgcca gattgcccat gcgccggtac aactgcaagc
     2341 gcagcgtgtc gttcggcacg tagtcttctg ggaggtaggc ctgcagcggc aggtcgaggg
     2401 tgacgcccgt gatcggcgca ggcggcggct cgctcccctc tcgcgcggcc ttcatctcct
     2461 cgaccgcgcg ccgcagcagc cgggtgtaca ggtcgaagcc gactgcgctg atctgcccgc
     2521 tctgccgcac gccgagcagg tcgcccgcgc cgcgaatctc caggtcgcgc atggcaatcg
     2581 aatagcccat gcccagctcg ctcgcctcgc gaatcgtgtc gagccggcgg cgcgcatctt
     2641 cggtcagggg cgagtgcgga tcggtgagga agtaggcgta cgcgcgctgc gccccgcgcc
     2701 cgacgcgccc gcgcagttgg tacaactgcg ccagcccgaa aaagtcgctg cgatcgacga
     2761 tcagcgtgtt ggcattgggt atgtccagcc cgctctcgat gatcgacgtg cacagcagca
     2821 cgtcgatctc gcggctgacg aactcgatca tcaccttttc cagatcgtgc tcgtccattt
     2881 gcccgtgacc gacgccgatg cgcgcttccg gcacgagcgc ggccagccgc ttctcgatgt
     2941 gctcgatgcc cagcacgcgg ttgtggacga agtacacctg cccgccgcga tccagctcgc
     3001 gcaggatcgc ctgccgcacg atatgatcgt cgtattcccc gacgtaagtc gcaacgggca
     3061 gccgctcctc cggcggagtg tcgatcgtgc tgatgtcgcg cacgccggtc aggctcatgt
     3121 acagcgtgcg cgggatgggc gtggccgtca tcgtcagcac gtcgacctct gcccgcatgc
     3181 gcttcagcat ctctttgtgc gtcacgccga agcgctgctc ctcgtcgacg acgagcaggc
     3241 cgaggttttt gaagacgacg tcgttttgca acaggcggtg cgtgccgatg acgatgtcga
     3301 cctgtccttc gtgcagccgg ttgaggatgt cgtcctgctc cttcggcgac ttgaagcgcg
     3361 acagcatctc cacgatcgtg gggaaggggg ccagccgcga ggagaaggtg ttgtagtgct
     3421 gctgcgccag caccgtcgtc ggaacgagca cggccacctg cttgccgtcc tgcacggctt
     3481 tgaacgcggc gcgcagcgcg acctcggtct tgccgtagcc cacgtcgccg caaatcaggc
     3541 gatccatcgg gcgcggcttc tccatgtcgg ctttgatttc cttgatcgca cgcagttggt
     3601 cttccgtttc gacgtagggg aaggctgctt ccagctcggc ctgccacggg gtatcggcgc
     3661 tgaaggcgaa acccggcgcc agctcgcgcg ccgcgtagag ttccaagagt tccttggcga
     3721 cctcaatggc cgcttgttgc gcgtgttcgc gcacctgggt ccagtccgcg ccgcccaggc
     3781 ggtggatgtc tggcggacgg tccgtcgcgc cgatgtactt gctcaaccgg tccgcctggt
     3841 ggatcggcac gtacagcttg tcgctgccgg cgtaggcgac ctgcaggaac tcaccgtcga
     3901 tgccttcgat cgtcagcttg accaggccct gaaagacgcc gataccgtgc tcgacgtgga
     3961 cgacgtagtc cccgggttgg atgtcggcga agaactgctc aggcgcaatc gcgcgcgggc
     4021 ggatgcgttt gcgcggctgc gggcgcgccc aaccgaatat ctcgccgtcg ctgagaacgt
     4081 gaagggagaa taactcggag gcggcccctt cggctccgct cagggcaggc ctgccgcgga
     4141 cgccgtgcag cgtccagccg tcgtccagcg ttccgtgcac gaactccagc gagcgtggcc
     4201 cgggcggaga ttggagatgt gagatggggt ggcgcatgtg atcgcgctcg gcccacagct
     4261 cggccaggcg ctgcgcctgc cggctgacga ccacgatcgc gtcgccgatc cgccggatct
     4321 gctcgacgtg atccaggaac gacttgagct ggccgccgaa gcgcgggcct ggaacgaagg
     4381 gtagtcgagt tgccgcgtcg tcgggtggtc ggatggtcgt gtggtcggga ggctgcgggc
     4441 cttcgtcggc aggagggact tcggccagcg agatgacgct tcgcgtcgaa aggctctcgc
     4501 gccactcttc ccaggtgaga taaggcagcg ggaagtcggg cggcagagtg cccgcctctc
     4561 ccgcgctgcg ccgcagatcg acggcttgtt cctcaagctc gctccacgcg tcttccatct
     4621 cgccggcgtc gtcgatcgcg atcaacgtat ccggcggcag atagtcggcg agcgaggcag
     4681 actgggtgta aaaatagggc agatagaact cgatgccccg aaacggcgcg ccggtcgcca
     4741 gcgccgaccg atcccgggcg agctgcgctt cgacgtcgtc gggcaggtgt tcaagactcc
     4801 aggcgcgcat ccggtcggct gccgccgggc cgtagcgcgc gagcgcttcg ctggcgggcg
     4861 tgatattcgc gacttcaacc gtttggtgcg aacgctgggt cgccgggtcg aaacggcgca
     4921 gcgactcgat ctggtcgccg aacaactcga tgcgcaccgg cgcgggggac gccaggggcc
     4981 agatgtcgag gatgccgccg cgccggctga actcacccgg ggcaatcacc gtcgtcatgg
     5041 ccgcgtatcc cagtcctgtc caaagctcca gcaccttttc caggtcgagc gcctggccct
     5101 gtttgagcgt gcgcgtgccg agcgccatgt tgcgcggggg cagcgtgcgg aacatgagcg
     5161 cgcgaacgca agcgacgacg acgagggacg agggccgacg gccgacggct gatggctgcg
     5221 cagagccggt gggtgacacg ccgtgatcgg cagccagcgt ggagaggacg ttcaggcgct
     5281 cggccacgac ctcactcgcc cacggcgccc gctcgtagaa cagcgcagcg ggttcgggga
     5341 agcgcagaag cgccgcgtca tcacccacgt aggcgcgcag cgcctcgtgg actatctgcg
     5401 cgcggtcgag gcgcgctgtg acatacagca tgggccgatc gagatcgcgg gtgagtgcgg
     5461 caatcaggaa tggccgggcc gggcgcagca cggccagcgg gcgcatgcca gcccaaggca
     5521 ctcgcagttt ttcagagaag tgccgacagg cgggtaattc actgagcaga gggaggagac
     5581 cggagaggtt catccgaccc attaatgtcg tccgcgacca ggttcggcgt gaggagaatg
     5641 tccagccggt cgagtttctc caccgatttg agcacgcgcg ctacggaagg cgtggggacg
     5701 cccgcgatcg attgcgctgt cttcacgaat cgatcggtcg tcatgaccga cggtttcaga
     5761 ccctgcctgc ggccacgctc ccagtttctg gtcaagacga tcgatatctg cgtcatggcg
     5821 ctgggtttcg acgattcgga ccctttggct gtcccttccc cttcacttcg ttcagggcca
     5881 gggcttcggc ttcccgcccc ccctcgcttt gctcggggtc gggggcttcg cagggcaggc
     5941 tgggggtcta ctcctgccgg agcgcctacc gtccttcgac tccgctcagg atcgccgcgt
     6001 tccgcgaccg gcgacccttc gaggctcaga gcttgccctg agccctgcga aggggcaggc
     6061 tttcgagcgc gtcgcggcgt gcgttccatc ttcctcaggc gctcgatcag cgcgtccatt
     6121 gtgagccgct tgggcgagat ttggtgcagc gccgcgacga tgctctccag tggcacctcg
     6181 tatcccatgc gctcgtgaac gcgcttgagg taagtgggcg ccatttccgg cgtgcagcgg
     6241 aaaatacgcg tgaactcgcg gatcagagtt tcatccttca tggtgatgcg ttctccacgg
     6301 tgcggttgtg tgcgttcatg gcgacgccga ttccgtgggc gacgaaggtc tcgatcgccc
     6361 gcgccgcgcg atccagtacg tcaggcagct cagcccgctc gctcgcatcg aaatcgtgca
     6421 gcacgtagtc ggctgcatcc atacgaccgg gcggccgccc gacgccaatg cgcaggcgcg
     6481 ggaaatcgcc gctgcccatg cgctcgatga tcgatttcat gccgttgtgc ccacccgcgc
     6541 cgcctttttc gcgcagccgg atcgcgccga agggtagatc gagatcgtcg taaatcacga
     6601 gcatgcgatc cggcggtgtc ttgtagaaac gcgccagggg ttggacggcg cgcccgctca
     6661 gattcatgaa cgtctgcggt ttggcgacga cgacgcgcac ttcgccgatg cgccccatgg
     6721 cgatgagagc gtcgttgcgt cgcttgctga agcgccaacc cctggagctg acgaagtgat
     6781 ccgcgacgtg ccatccgaca ttgtggcggt ttccagcata ttcccggccc ggatttccca
     6841 ggccgatgat gagcgcggtc ggcgcgctcg acccttcgcc ttcgcccccc gctcccttcg
     6901 gtcggggact tcgcagggca ggctctggag tccgcttgcc gcgtagtcgt tcaagaagat
     6961 gcatatgcgc tcgaaactct actgattcga ccgttctcgc atttgcttca tgatcagccg
     7021 gaacgcccag cgcgccagcc atgccgcgcc gagcaagagg aacgcgccgg cggcgcacat
     7081 cacgccgtcg gtaaatgccg aaccgaagcg gctgagatcg atgctcgacc cgccgcccct
     7141 cgacgaagac gcatcgccgg cgctgccgcc gcctgccagc gtgggcgctg gtgtcggcgt
     7201 cggcggctgc tcgatgacga ccgtggactg gatggttccc tgagtctccg gctcgaaggt
     7261 cggtggggga ggcacgctcg tcggcgtcgg cgcgggcgtt tcggtgggcg gcgtgccgac
     7321 gatcagcccg ttggcgaagt cctcggcggc gatgctgccg tcggaacgga agacgcgcag
     7381 ccgaagctga taaagaccgg gcggcaggtt ggcgtcccaa agcccgattt gggcgttgac
     7441 caggactacg tcgggtgtgg cgaaagtcgt ccaggtatcc gatggattcg gatcaggccc
     7501 gaaggccagc tcgtagcgag cgaagtcgct cgccgtggcc gtgccgaaga ccgggacgag
     7561 gcccgacacc tgctggccgt ctgccggaga aatgatgacg gcctgatcgc cttgcgcctg
     7621 cggcgcgccg gcaacaggca taacacacgt cgcggccacg agcagcgccg cgagcgcgcc
     7681 tccgaaatat cgctgcatga ttccctcgat gcgagaagta caatagaaaa gtgtaccata
     7741 ccgggtaggg atgagcaact ggatgggcac accccatctg ctgctcacgt cgtcaatccg
     7801 tctgccccgc cgcgatcggg atgctgtagg aatacactgg accatcgggc gattgcagag
     7861 aataacctcg cgcgtgaacg ctgtcgaaca agccaacctc caacgtgtac gagccgaggg
     7921 tcacgtcttt cggaacaggg atactcacgc gctccacgta gatgtcgcct ggctcccagc
     7981 ggcggctcca ttgaccgtgg ccgttctctt cggcgagctt ctgcccttgt gcatttaaga
     8041 gatgatagaa agcgctgatg aaccattcct cacgtccagg ctgcaacgca tcgacgcgcc
     8101 agtaggttgt gacatcgatc ccgtctcccg gctgcgcatt gtcttccagg gtatagccga
     8161 gcagcgacag cccactttcg ctcggccagt caaccttcac gcctggcagc gcgagggctg
     8221 ccgatcgatc atagggttgt acacgcacga aggaggtatg cgcaccgtcg gcaaattgaa
     8281 gatcgtactc cacgaaggat tcttggtgag gccccaggat acccggctct actgaggtgt
     8341 taaccaagac gtacaagagt ggttcgctgc ccggcagtac cacgaagttc gggaagtcga
     8401 gaccgctcag cgttttcaca cgcacgccac tcatgccgct gaggatctct gactggccgt
     8461 cggcgacgat gcgacgcggg agacggttgg cttcggtcag gtcgcgaacg gcctgcccca
     8521 ccttggccgc cggctccagt gcccagccgt tgaacctggg cggcgtcggt gatcgcgcga
     8581 cgccctcatt catacgattc aagttgacgc caaaaaagac cgc
//
